LOCUS       DAGSWI010000001        74734 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.001, whole genome shotgun sequence.
ACCESSION   DAGSWI010000001 DAGSWI010000000
VERSION     DAGSWI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 74734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.001"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            220..1146
                     /locus_tag="MJF80_000001"
     CDS             220..1146
                     /locus_tag="MJF80_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBZ0278690.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(1266..2264)
                     /locus_tag="MJF80_000002"
     CDS             complement(1266..2264)
                     /locus_tag="MJF80_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBZ0278691.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            2368..2805
                     /locus_tag="MJF80_000003"
     CDS             2368..2805
                     /locus_tag="MJF80_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBZ0278692.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            2891..3478
                     /locus_tag="MJF80_000004"
     CDS             2891..3478
                     /locus_tag="MJF80_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBZ0278693.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(3778..4398)
                     /locus_tag="MJF80_000005"
     CDS             complement(3778..4398)
                     /locus_tag="MJF80_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBZ0278694.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(4406..5173)
                     /locus_tag="MJF80_000006"
     CDS             complement(4406..5173)
                     /locus_tag="MJF80_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBZ0278695.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(5214..6035)
                     /locus_tag="MJF80_000007"
     CDS             complement(5214..6035)
                     /locus_tag="MJF80_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278696.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(6394..8304)
                     /gene="phnW"
                     /locus_tag="MJF80_000008"
     CDS             complement(6394..8304)
                     /gene="phnW"
                     /locus_tag="MJF80_000008"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBZ0278697.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYVQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(8583..9368)
                     /locus_tag="MJF80_000009"
     CDS             complement(8583..9368)
                     /locus_tag="MJF80_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBZ0278698.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPISIDAMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKAYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(9585..9815)
                     /gene="dltC"
                     /locus_tag="MJF80_000010"
     CDS             complement(9585..9815)
                     /gene="dltC"
                     /locus_tag="MJF80_000010"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBZ0278699.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(9834..10982)
                     /gene="dltB"
                     /locus_tag="MJF80_000011"
     CDS             complement(9834..10982)
                     /gene="dltB"
                     /locus_tag="MJF80_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBZ0278700.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(10982..12496)
                     /locus_tag="MJF80_000012"
     CDS             complement(10982..12496)
                     /locus_tag="MJF80_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBZ0278701.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(12617..13807)
                     /gene="dltD"
                     /locus_tag="MJF80_000013"
     CDS             complement(12617..13807)
                     /gene="dltD"
                     /locus_tag="MJF80_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBZ0278702.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(14095..15114)
                     /locus_tag="MJF80_000014"
     CDS             complement(14095..15114)
                     /locus_tag="MJF80_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBZ0278703.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(15290..15829)
                     /locus_tag="MJF80_000015"
     CDS             complement(15290..15829)
                     /locus_tag="MJF80_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBZ0278704.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(15930..16610)
                     /locus_tag="MJF80_000016"
     CDS             complement(15930..16610)
                     /locus_tag="MJF80_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBZ0278705.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTEIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILETLLEVREYLKSL
                     NSKECHNL"
     gene            complement(16639..17928)
                     /locus_tag="MJF80_000017"
     CDS             complement(16639..17928)
                     /locus_tag="MJF80_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBZ0278706.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            complement(18377..19978)
                     /locus_tag="MJF80_000018"
     CDS             complement(18377..19978)
                     /locus_tag="MJF80_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBZ0278707.1"
                     /translation="MIYDLILKNGFIIGGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGINGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(20089..20766)
                     /locus_tag="MJF80_000019"
     CDS             complement(20089..20766)
                     /locus_tag="MJF80_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278708.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(20779..21474)
                     /locus_tag="MJF80_000020"
     CDS             complement(20779..21474)
                     /locus_tag="MJF80_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBZ0278709.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(21526..22725)
                     /locus_tag="MJF80_000021"
     CDS             complement(21526..22725)
                     /locus_tag="MJF80_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBZ0278710.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(22986..25031)
                     /locus_tag="MJF80_000022"
     CDS             complement(22986..25031)
                     /locus_tag="MJF80_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0278711.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(25442..26251)
                     /locus_tag="MJF80_000023"
     CDS             complement(25442..26251)
                     /locus_tag="MJF80_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBZ0278712.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(26335..26868)
                     /locus_tag="MJF80_000024"
     CDS             complement(26335..26868)
                     /locus_tag="MJF80_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBZ0278713.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(27018..28367)
                     /locus_tag="MJF80_000025"
     CDS             complement(27018..28367)
                     /locus_tag="MJF80_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBZ0278714.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMFIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(28379..29761)
                     /locus_tag="MJF80_000026"
     CDS             complement(28379..29761)
                     /locus_tag="MJF80_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBZ0278715.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            30005..31219
                     /locus_tag="MJF80_000027"
     CDS             30005..31219
                     /locus_tag="MJF80_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBZ0278716.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            31396..33309
                     /locus_tag="MJF80_000028"
     CDS             31396..33309
                     /locus_tag="MJF80_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0278717.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFNDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            33580..34554
                     /locus_tag="MJF80_000029"
     CDS             33580..34554
                     /locus_tag="MJF80_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBZ0278718.1"
                     /translation="MHILKSVKKIPGGLMVCPLLFGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            34575..34850
                     /locus_tag="MJF80_000030"
     CDS             34575..34850
                     /locus_tag="MJF80_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBZ0278719.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            34863..36026
                     /locus_tag="MJF80_000031"
     CDS             34863..36026
                     /locus_tag="MJF80_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBZ0278720.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYIVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKTGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            36363..38642
                     /locus_tag="MJF80_000032"
     CDS             36363..38642
                     /locus_tag="MJF80_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBZ0278721.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQGISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(38737..40077)
                     /locus_tag="MJF80_000033"
     CDS             complement(38737..40077)
                     /locus_tag="MJF80_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBZ0278722.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(40089..40871)
                     /locus_tag="MJF80_000034"
     CDS             complement(40089..40871)
                     /locus_tag="MJF80_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0278723.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(40886..41908)
                     /locus_tag="MJF80_000035"
     CDS             complement(40886..41908)
                     /locus_tag="MJF80_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBZ0278724.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGERNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(41914..42927)
                     /locus_tag="MJF80_000036"
     CDS             complement(41914..42927)
                     /locus_tag="MJF80_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBZ0278725.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(43055..43993)
                     /locus_tag="MJF80_000037"
     CDS             complement(43055..43993)
                     /locus_tag="MJF80_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBZ0278726.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            44263..44727
                     /locus_tag="MJF80_000038"
     CDS             44263..44727
                     /locus_tag="MJF80_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278727.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(44839..45156)
                     /locus_tag="MJF80_000039"
     CDS             complement(44839..45156)
                     /locus_tag="MJF80_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBZ0278728.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(45169..45900)
                     /gene="chbG"
                     /locus_tag="MJF80_000040"
     CDS             complement(45169..45900)
                     /gene="chbG"
                     /locus_tag="MJF80_000040"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBZ0278729.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKSVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(45926..47236)
                     /locus_tag="MJF80_000041"
     CDS             complement(45926..47236)
                     /locus_tag="MJF80_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBZ0278730.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(47313..48626)
                     /locus_tag="MJF80_000042"
     CDS             complement(47313..48626)
                     /locus_tag="MJF80_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBZ0278731.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(48648..48959)
                     /locus_tag="MJF80_000043"
     CDS             complement(48648..48959)
                     /locus_tag="MJF80_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0278732.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(49159..49863)
                     /locus_tag="MJF80_000044"
     CDS             complement(49159..49863)
                     /locus_tag="MJF80_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0278733.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(49954..51315)
                     /locus_tag="MJF80_000045"
     CDS             complement(49954..51315)
                     /locus_tag="MJF80_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBZ0278734.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAIYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            51814..52968
                     /locus_tag="MJF80_000046"
     CDS             51814..52968
                     /locus_tag="MJF80_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBZ0278735.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRSAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(53050..54768)
                     /locus_tag="MJF80_000047"
     CDS             complement(53050..54768)
                     /locus_tag="MJF80_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBZ0278736.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(54773..55762)
                     /locus_tag="MJF80_000048"
     CDS             complement(54773..55762)
                     /locus_tag="MJF80_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0278737.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(55858..56907)
                     /locus_tag="MJF80_000049"
     CDS             complement(55858..56907)
                     /locus_tag="MJF80_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBZ0278738.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(57185..57853)
                     /locus_tag="MJF80_000050"
     CDS             complement(57185..57853)
                     /locus_tag="MJF80_000050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBZ0278739.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(57859..59232)
                     /locus_tag="MJF80_000051"
     CDS             complement(57859..59232)
                     /locus_tag="MJF80_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBZ0278740.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(59229..61007)
                     /locus_tag="MJF80_000052"
     CDS             complement(59229..61007)
                     /locus_tag="MJF80_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBZ0278741.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEIHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(61124..61447)
                     /locus_tag="MJF80_000053"
     CDS             complement(61124..61447)
                     /locus_tag="MJF80_000053"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBZ0278742.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(61434..62411)
                     /locus_tag="MJF80_000054"
     CDS             complement(61434..62411)
                     /locus_tag="MJF80_000054"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBZ0278743.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(62426..62989)
                     /locus_tag="MJF80_000055"
     CDS             complement(62426..62989)
                     /locus_tag="MJF80_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBZ0278744.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(63041..63532)
                     /locus_tag="MJF80_000056"
     CDS             complement(63041..63532)
                     /locus_tag="MJF80_000056"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBZ0278745.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(63535..65460)
                     /locus_tag="MJF80_000057"
     CDS             complement(63535..65460)
                     /locus_tag="MJF80_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBZ0278746.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(65462..65773)
                     /locus_tag="MJF80_000058"
     CDS             complement(65462..65773)
                     /locus_tag="MJF80_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278747.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(66403..66678)
                     /locus_tag="MJF80_000059"
     CDS             complement(66403..66678)
                     /locus_tag="MJF80_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HBZ0278748.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(66793..68202)
                     /locus_tag="MJF80_000060"
     CDS             complement(66793..68202)
                     /locus_tag="MJF80_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBZ0278749.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(68573..69235)
                     /locus_tag="MJF80_000061"
     CDS             complement(68573..69235)
                     /locus_tag="MJF80_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278750.1"
                     /translation="MIDVSIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(69489..71708)
                     /locus_tag="MJF80_000062"
     CDS             complement(69489..71708)
                     /locus_tag="MJF80_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBZ0278751.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLNEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFVAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LSEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIQMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRKHGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(71984..74638)
                     /gene="adhE"
                     /locus_tag="MJF80_000063"
                     /gene_synonym="adhC"
     CDS             complement(71984..74638)
                     /gene="adhE"
                     /locus_tag="MJF80_000063"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBZ0278752.1"
                     /translation="MAHKEQVIIENKKQNEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
BASE COUNT        24797 a        13062 c         8665 g        28210 t
ORIGIN      
        1 ttaataggat ttatactaat tgaaaaaata ttataagttg ttaaatttaa atatctaaac
       61 tcaattatat ctgtacaatt gtaatttttt aaatcatgat gtattatttt aaatatacag
      121 ttacattatt attcacaata taagtatctt agttctcaaa taacatctca tgtaatctat
      181 tcaatgcaaa aaatttaaat attttttagg aggtttttta tgagcaagga aaataaaaat
      241 gtaagaattg ctgttgtcca ggcgtcacca gtcataatgg atttagaaaa aactgttgaa
      301 aaagcattag gattaattaa agaagctggt agaaaagggg caaatatcgt agttttccct
      361 gaagcattca tacctgcata tccaagaggt ctttcatttg gatttgttgt tggaagtaga
      421 acaatggaag gtagagaaga ttggaaaaga tactatgata attctgtacc agttcctagt
      481 gctacaacag atttattagg aaaagcagca caagaagcag gagtatatct atcaatgggt
      541 ataacagaaa gagatggtaa tgatataaat tgtacccttt attgcacaaa cttatttttc
      601 tctccagaag gtaagctaat tggtaaacac agaaaactta aacctacagg aacagaaaga
      661 tgtatttggg gagaaggaga tggaagtact cttacagtag ttgatactcc atatggaaaa
      721 atgggttcgt tgatttgttg ggaaaattat atgccattag ctagaacagc tctttatgct
      781 aagggtgtaa aactttacat agcccctact gcagattcaa gagaagagtg gcaagcaact
      841 atgaaacata ttgctttaga aggaagatgt tttgtaattg gatgcaatca atatgttgaa
      901 aaaaatatgt atccaactga ccttaactac tataaagagc tagatgcaga accagaaatt
      961 atgtgtccag gaggaagttg tatagttgac ccttttggaa aatatgtagc aggtcctatt
     1021 ttcaataaag aagaaatgct aatagctgat ttagacctag aaaaaatagt acttagtaga
     1081 ctagattttg attcagaagg tcactattca agaccagatg tatttgaatt aattgttcat
     1141 gaataatctt tttacacaaa catctacata aatcttttta cataaaaaaa tgagatactc
     1201 ataagtaaat ttcaagtatc tcatttttgc ttaaaaccta taatcctaat tataaattta
     1261 ttcacctata ttttttctaa atcaactact gttcttccaa catgttttcc tgccattatt
     1321 gaatctatac tatcatttaa ctcttccaat gatactacat ttaccatatc atctaaatca
     1381 tctaatttcc attcatttga taataaatcc catattttta atcttaagtt cattgggcaa
     1441 tccacagaat ctataccata taaagtaatt ccctttaata taaatggata tactgaactt
     1501 gtaaaatcaa ctcctcctgc attaccacaa gttgttactg ctccacaata attagttgtc
     1561 tttattgctg ttgctagagt atttcctcct actgtatcta ttactccagc ccatcttggt
     1621 ctaagcagag gtcttcctga gttatcatcc acttctgacc tatgtataac atctttaaca
     1681 cctaagttta gtaatacttc tttttgttct tctaattttc ctgttgaaac tacaactgaa
     1741 taacctaatt taatcaatat tttagttgca attacagcaa ctcctccagt accaccagtg
     1801 actaatacat ctcccatatt cttgtctaca gaatctataa atttatacac agataaagct
     1861 gctgtaaacc ctgctgtacc ataatccatt gcttctttta gtgaaagatt tttaggaagt
     1921 ttaactaccc aactagaagg aactcttatg tattctgaaa aaccaccaga agtattcatt
     1981 cccaagtcat aacctgttac taaaacttct tctccaattt taaaatcatc acaactacta
     2041 gattctacta taccagatgc atcaattcct ggtgtatgag gatactttct cgtaacaccc
     2101 ttattaccat ttgcagaaag tgcatccttg taatttaaag aagaatattt tacttttatt
     2161 aaaacttcgc cttctggcaa tgtatccact tctctttcaa ctatatttct tctaaaattt
     2221 ccttcttctt ctgtaatata tagtgattta aattttttat tcataaattt cctcccaata
     2281 catttagttt tatgttaaac taattatagt ttataaatag ttttatgtca aactaattta
     2341 aatagattaa agattggaga ataatttatg gaagataaat atattgtaca tttcataagt
     2401 aaaacaaagg ctagtatgat taaattcatt gaaaataaac tcagtaaaaa tggtttaggt
     2461 gagttaatac ctactcatgg aaatatttta actgcacttt atgaaaataa tggaatactt
     2521 acaatgaaag aaattgcagc taaaatagga aaagataaat ccactgttac agtgttggtt
     2581 aataaactta taaacctagg atatttagaa agacaaaaat gtacaaatga caaacgtatc
     2641 acgtatataa agcttacaaa aaaggcactg cttatagaag atacttttaa tagcatctca
     2701 acacaagtta aagagacggc ataccataat attacagaaa atgaaaaaca agaatttctt
     2761 agaattttaa aaaaaattaa tgataatttt aaaaatgctg actaaattga gcataattat
     2821 atctcctatt ggaataataa ataaaaagaa tcaaatattg taaaaattta ttatgataga
     2881 gaggtagaat atgaatttaa aagatagtaa aactaaagaa aatttattaa gagcttttgc
     2941 aggtgagagt caagctagaa atagatacaa tatttctgca tctgtagcaa aaaaagaagg
     3001 attatatata atagagtctt tatttaatta cactgctgac caagaaaaat cacatgcaaa
     3061 acaattcttt tctaaattaa aagaatttgc tggtgaaaat ataagtatat caggagcata
     3121 ccctgtagat ttatatgaca agacagcaca acaccttaaa gctgcacaac ataatgagct
     3181 tcaagagtgg gatgatgtat ataaaaactt tggaaaaaca gcacgtgaag aaggatttga
     3241 atctatagct aagttatttg aaaatatagc tgaaatagaa aaagttcatg gtgatagatt
     3301 tggtagatat gctactgatt tagaagaagg tactttattc agaagaaatg aagatataca
     3361 atggatatgt acacattgtg gccatataca tataggtaaa gaagctccaa aagcttgtcc
     3421 tgtatgtctt catccacaag gatatttctt agattttaaa aactcattat ttgaataaat
     3481 taaggcaata attctattta aacaatttac tatatttata tcaagtattt ttataaatac
     3541 tgtattatct taataacaat aaaatgaacc attgatatta ctataagtag aaaatgtagt
     3601 ttaaatacaa tttatagtaa ttcatcatgg ttcattttat tatttactaa tccctataac
     3661 ctacttaaat ctatctttaa cttttacaca ctcacataaa atctcatctt taaactaaat
     3721 atttaaataa agtgtatttt aagtatatta ttataataca ttatataaga taatacacta
     3781 tgataacttt atacttatat catttatttc ttttgtcaag tcaacctcaa taatcttaac
     3841 tacatctttt tcaaacttta cttttttata atccaaacat cctactacga atggaactcc
     3901 aaactttcta aacaaataaa tctttccact ttcattgtat attgatgtaa aattttcttc
     3961 tattatattc actatcaatt ttaactcttc aaaagttatt atagatttaa ctaaaaactt
     4021 ctctattatg ctcaatataa gcagttcttt tttacctatg ctttgctctt ttttatataa
     4081 attttgaaat atttctttgg tataattaca tataacttct ttactgagaa taaaatcaat
     4141 gtcaaattct ttattaatat cctttttcat aaacatattt gcaatatcat caagagaagt
     4201 ctcttctttc atagacaata ttaactctat tctttctagt attttatctc ttggaaagaa
     4261 agtctcttga cctgtaaaag ctgatttttt tataaaccat tcctctggta ttatgttttt
     4321 tcttttccat ctatataatt gaccataaga tatattagtt ttactcaata aatctttctt
     4381 agatattaat tcttccataa cttttctagc ccacctttat actgtttttt acagtagaat
     4441 tatctgaaac tgataaatta tcactgtatt caatatttcc aacgtcacaa ttatctacta
     4501 gagatatgct tttacctcgt atatctttta ttgatgcatc ttctaattca atagtatctc
     4561 cctctatcaa attacattta aactttcctg aaaaaatatt aatgatttta attaatgaaa
     4621 aagcatcttt gcttcttcct atttttatat tagttgcccc tatctcttta caataagaac
     4681 ctcctcctga ataaacatat atattttctc catttaaaag accatcacat tttattactc
     4741 catatacctt tatatcttca cattctatat ttgatttatt tctcatttcg ccatagattt
     4801 ttatatcttg tgcatacata ttttcattta cacttaattg tcctcttatt gtaacattat
     4861 tacatttaag tttgccatcc actttctgtt gagcagttat aactaagtca tctatttctg
     4921 catctccctc tatattaagt tgaccattca ctcttacacg ctctgcttta agattgcctc
     4981 ttataatagt ttttccgtta acaacaaaat tttttatatt gatgtcacct tcaaattttg
     5041 catctccaag gacttttact tcgttacttt taacatttcc ttttgattta gaacttccca
     5101 cgatttttat attttcatac tcaccactac ttataaagcc ttctccaagt atttttatag
     5161 accctttttc catattatta cctcatttta aattttattt ttaaacctat ttattactta
     5221 tccttcttta attttatttt ttcacttata ttctttattt caccaactat tgaatcttca
     5281 tcaatctcta tactttcact gtactccaaa ctttctacat aacatccctt gcctatttta
     5341 atattttttc ctcttacaac tttgacttca ctattttcta agcttatttc atctgcttct
     5401 ataatatttg caaacaattt actttctgta tactttctaa ttgataatag acctaaactt
     5461 attaaatttg aagacacttt ttcaaagttg atatgctttt tttctagcat acgaaatttt
     5521 ttgcaataat ctccatgccc cttttttata tttatactac ttgctccaac ttcattaaaa
     5581 ttagaagttc catttaaact aatattaact tcttcacaat tcaaaaatcc attacactcc
     5641 aagattccac ttaaattgat attttcacaa tctatattct catttgcttt taaatatcct
     5701 tctccatgaa taatatccga ttttatactt ccttctacat taataattcc actagtatca
     5761 atttcaacag aattaatatt tcctgtacat ctaagaagtc ccgatatctc aacttttgat
     5821 gccagtatgt ttccttctac tttacttaaa ccagatgtaa aaaattcatt acattctgtt
     5881 gtcccaaaaa cttttgctga accagaagaa tcaaatttac tacattttac acttccttct
     5941 attttgcctg ttcctgatat attaacatta tcaaaaattc cacctttaca ttttccaaca
     6001 ccatcaaatc ttagtgaact taattcttca ttcataatga tatcctttct ttaccgtatt
     6061 ttatctttaa acctaatata acaaaacatt gttacgttgt caagtatgat aataatactt
     6121 agtaaagcaa ctatcattta ttttctataa ataggcataa aaataatagg gaaattcttt
     6181 atttaaacta taaatctaat ttcttagatt gtggtttaaa taaaatatat ccctattact
     6241 caatctttat atattcattg tataaacttc taaaatataa gtctttaaat tctactgtca
     6301 atacttttta tacactgttt gtgctatcat ctcacacacc taaaggagtg ggctttccgc
     6361 actattctgt aacctacaag acactatctt cttctatcta ttgataaatt tttctattac
     6421 atcagctagt ctctccatat cagttggata aacttcacct atgctaccta ttctaaatgt
     6481 atctatatca gtcaatttac ctggatagat aacaaatcca ttatctttta aataagtata
     6541 aaattccata aattcaaact ttgcattttt aggatataag aaagttgtta ttacaggtga
     6601 ttgagcatta tcattcacca aagtatcaaa tcctaaagat tttaatcttg aagctattgt
     6661 aaactgattt tctttatatc tagcatatct tttttcaaca gaaccttctt cttccaattc
     6721 taataaagct tgataaaaag ctcttacaac atgtgtaggt gaggtaaatc tccatttacc
     6781 attatttttc tccatagtct cccattgcac atacacatca agagataatg atttagctat
     6841 acctttacat ttagataatt tctctttatt agctattata aaaccaaatc caggtactcc
     6901 ttgtatacat ttgttagaac tactaactaa aaaatctata ttaaaatctt caacatcaat
     6961 ctcaattccc ccaaaactac tcatagcatc aactatataa attttgttat attcttttgc
     7021 aagtttccct acctcttgta taggatttaa tctacctgta gtagtttcac agtgaaccat
     7081 tgatatatgt gttatatcct tgttttcttt taataaattt tctactacat ttaaatccac
     7141 tgcttctata tctttaaatg tgcaatcaac aaattctatg ttaagataat tacatatatc
     7201 tttcattctc tttccatatg ctccatttgc aattacaaga agtttaccat cttttgaaat
     7261 agtacttcct attatagctt ctactgaaaa agtcccactt ccttgcatta atacacttgt
     7321 gtatttatct gtattttggg ttgctagaga tactaatctt cttctaacat cttgtactag
     7381 gttattatat tcaacatccc aagtacacca atcttttaac atagatgctc ttactgattt
     7441 agtagtagat agtggtcctg gtgtaagtaa tatgtaatca ttttctggta caaaatctga
     7501 tttaatagtt tcattctcta tttttattag tatatcttct aactcagcca tagtttctat
     7561 tacaaaatgt gctcctgcct ctttaaattt ctttgaaact atactcatct tagcttttaa
     7621 ttcttcctta tccatgttct ctacttcttc ttgtgtaagc cctaactcac ttgaaccttt
     7681 tattacagca acactccaca ttcctgcatt tacgccttct tttacatcac ttattgtatc
     7741 tcctactttt accatagaac tcataggaga cacccctaaa gcttcagcat ttttatagca
     7801 catccaagga tatggtcttc cttgagatac ttctgatggt gttacaagaa aatcgggtga
     7861 gtatcctttc tttgctgcat ttggttcaac tatgttcatc atctctcttg tataacccgt
     7921 tgtagaacct atttttaatc cattttttct caacttttca atagtttcaa caacatgagg
     7981 aattggagtc gtatactctt ctaatgtctc aaataacatt ggctcaaatt cagcataaag
     8041 ttcatttaca tcatcttcag ttggcacttt accaaactta tctgaccaaa tattctttat
     8101 tctatccatt tcacacattt ctcttatatg gtctattttt aattttccca taggttttct
     8161 tgcttcttcc attgttacat ctatacctct tcttttaaat atctctataa atacattaag
     8221 aggtgcaaaa catccataat ctactgttgt tccagcccaa tcgaatacta ctgcttttat
     8281 tttttctcca tatatctttt tcatatttat agcctccata gattcttttt agaatttttg
     8341 tgtttttaat tgtttttact tttaaagtat aactttcata tgttaattat acatcatatt
     8401 taggttaaat atatattaaa tttctgtttt tagttgtatt tagttgtttt tattttgtat
     8461 catattccca taaaatccta agttacaatc taaaatttac tttctaatct taatctatga
     8521 actgactata ttttatgaat ttagttgtat tgagcatagt tgtattatat aaaactaagg
     8581 gtttattttg cttctatcca tgttatattt atctcattta acttatcctt ccattcattc
     8641 ggaggttcta catcacaaat aacaaaatct aattcctcta aatctgctat cttatacata
     8701 ctagcaattc ctattttaga actatcacat aatacaaaac tttcctttga attttttata
     8761 tatgcttttg acaacaatgc cttattttgg tctatactac taattccaaa actatcaagt
     8821 ataccttcac tagatataaa agctttatct acgtataagt ttttcatagc atctattgat
     8881 attggacatg ctgtcctttg gtgttttaaa tttacttttc caccaataaa tataacttct
     8941 ccatcaaaca aaccttgatt ctcgtaagaa atcaattttg acagaactgg ataagagttt
     9001 gtaataattt ttaaattgtt tacagatatt atataatcta caatctgcaa attggtagta
     9061 ccttcatcta tgataattga ctcattgtca tttacaaact tagctgcata tttagcaata
     9121 ctttgctttt tatctaggtt agtattgttt ctctttatat gttctggttc aatcactcca
     9181 taatttattt ttatagcacc accatatacc ttttttaatt tattttcatt ttctaattct
     9241 tccaaatatc ttcttattgt ttctgatgat acttctaatc tttttactaa ttctttagtg
     9301 tttaccttcc cttttgaatc tagtaattta agtatttctt tttttctatc tatcgaattt
     9361 aaagacattc attttcctcc taaatataat atatttttct cctataatat tacaatgata
     9421 cctaagattt atgaaatcat atttatgtat taaatttttt acttaattaa agttattttc
     9481 aaaagacttt gtattatcat taaaaaagtc catcaaatat tagtatcaga tggactttgt
     9541 aaaattttat tttataaatt aatatttaaa gtctaactac taatttattt tcttgtctcc
     9601 aagaatttta ctaaattatt tacagtagac atatcttttc tctctaaatc agttggttga
     9661 agctctatcc ctaatctatt ttcaatttca agtaaaactt caattatagc taatgagtct
     9721 aaaagttccg tctcaaataa attaaggtct aaatcttctc ttatctcatc agtccctaat
     9781 acatcttcaa atatttctat aactgtttct tgcataaatt ttcctcctta aaattaagct
     9841 aaataacctg agaatatcag caatccaaaa cacacaatat ggaatgtaat gaaaatctgc
     9901 actctctcga accatttatc ttttttatgt tttttataaa atttagattt tcgctgatat
     9961 atatcagtta acacaagtgc caatccttgg tatacaccat aagcaacata ataccaagtt
    10021 agaccatgcc aaaatcccat tgttatcatg gttatcatct gagccacatg agcagcagta
    10081 gtccttttct taaatctttt ctttctcatt gatgacataa caaatctaga aaaaatataa
    10141 tctccaaacc atcttgaaag acttatatgc catcttgtcc agaactcttt catatctttg
    10201 ctgataaatg gcttgtcaaa gtttataggt acttgtattc caaatatgta ccctgtacct
    10261 atagcaaata aactatatcc tgcaaaatca aagaacaggt ataaactata tgcgtacata
    10321 taacttaaaa tatttataaa actcatatct ttaggtattc ttgataccca atatgtattt
    10381 atcaaaaatg ctataacaaa cttataacct actcccatga caatattttt tattcctggc
    10441 agtaagtatt cttctatata ctcttttcta gagatttgtt tttccaagtc ctgttcaaat
    10501 cttcttgacc tatcaatagg tccagaactt aaagttggaa aaaataacat aaaataaagc
    10561 atctttgaaa ttttaacttc ttttatagct ccatcataaa tttcaataac catttgtata
    10621 gttctgaaat ttaagtatga tattccaata aatccaatta ttccaaaaga agtaactgga
    10681 gatattttat ttattattat tgggagcatt gatgcaaaca agaatcccca atatatgtat
    10741 ttatttttag tttttcttct tacatattca tatcctttaa ctattattac ttcaagaata
    10801 ataaatatta ccaagtactt tagttggaca tcaattccta ctattaagaa tatcataaac
    10861 aaacttgcca acataccata atactttata ttcttgccca ttagacctaa aattactgct
    10921 ggtattgatg ttaataataa tatataaaga taaaaataat ctccatattg tgaaaaaatc
    10981 attaaatttc ctccataagt ttctttctgt ctattttacc attgatattt gttggaaact
    11041 cagagattat tgaaatattt cttggaatca tatatgaagg tatatacttt ccaagttcct
    11101 tttttattat catcccattt ttaatattac ttaaatcatt cttttcattt aactcaacaa
    11161 taccttttaa gtaagcaatt ttttcgtcct tatatactgg taaaactact gcatttttta
    11221 cattatgtac ttttctcaaa ttattttcta tgtcttctat ttcaattcta aatccattta
    11281 atttaatctg aaaatctttt cttccacaat aatatatatt tccatctaaa agatatccca
    11341 tatctccagt tttataagct ctccacttta caccatctat ttcatcatag aaaaatactt
    11401 catcagtctt ttctttatta ttgaagtaac ctttagaaac tgatggtcca attattataa
    11461 tttcaccctt ttcattttct ttaagctcat ttccatcttc atcaagtatt tttattttac
    11521 aatttgacat agggtatcca actggaagac ttttttcatc atctatagcc ttttgagtca
    11581 tatcatttac acttacacca acagtagcct cggttggtcc atatccattg ataattcttg
    11641 tgtttggaaa tctactcata agttctttag taagattttt tgaaagtgct tctcctatga
    11701 atatcattga ctcaaggtta ggaagcattt tactattaaa ctctttttca gttatacata
    11761 ctcctgcaaa agaaggagtt gacacccata cagctatatc tgagatacta aattgtctaa
    11821 atagttcttt atagtctgct aatacatctt ttgatattga aaataatgta gctccatgga
    11881 taagaccagg gtatattgtt gtaactgaaa ggtcaaaaga atatgctggt tggttcatta
    11941 ttactttttc acttccatct atgtttagat atgggcttat ccaatctgaa aagctatcta
    12001 aattgtttga acttatttga accccttttg gctttcctgt acttcctgaa gtaaatagta
    12061 tatatgcatt ttcatcatct tttacccagt tttctttatc taatgatttt ccctgatatt
    12121 catttattat ataatttaat ttatccatat caattacatt gatatctcca aaatttcttt
    12181 catcactaaa gttaaataat acctttggtt ttatctcttt agtgacttca aaaactctat
    12241 caattggaaa actaatatca agaggcacat acgctctacc tgattttaaa gctcctatca
    12301 tacatgccat tatcatgttt tctttatttc cataaattac tataggagta tcttcttctt
    12361 tgtatacatc ctttagaaat actgatatag catctgaata ttcatttaaa tctttataag
    12421 aaagtttatc tccattacac attagtgctg ttctatctgt atttgaatat ttttttattc
    12481 cttcaataat tttcatataa gaatgctcct ttaaaatccg ttataaataa atggcaattc
    12541 tttaaatgga gtaaatgtta gaactcctat cgccaatatt ataaccatag ttaaacaaat
    12601 actagttaac aaatctttat tgttctttaa atattttaaa taatctctca cagacatcta
    12661 cccatccttt cgtaccaaga tgcattacat cccttaaata atacttatct gaccctttat
    12721 ctttaaggtt tagaacttca aagcctttgc tttctgctat tttttgtgct ttatcgtagt
    12781 actgatttct ttctttttca gaaataccag tcaaattata gaatttatcc atacttggga
    12841 ttaatacaac aactggctta atacctaagt cagtacatac atttagcatc agtttataat
    12901 tttcaaactc cttagatgtt aataaattta catctttgtc tcttcctttg tattgctcta
    12961 taccatcttt aaaatgctct ttataatagt acttatcaat atttagtgga tttttaccaa
    13021 ctcttttttt agcatcttca atggctttct ttctctcata tgaccaattt attggtcttt
    13081 ttctttttgt tgctctcttt ttatctagct taattaatcg tttataaaga ataccctttt
    13141 ctttaagagc tatacaatac tttcttcctt gaaaataagg ctctaaaagc accttttcag
    13201 ctttttcagg aaatgtctta ggctcatata gcttagcata taatgcttct gctttatact
    13261 cgtcacttcc ccataataac ttacttgatt tttttgcata ttcaatctta ttttgtttag
    13321 atatttttgg attatctaaa aatcgataaa attgtattgg tgaaaatcta gactgataat
    13381 ggtgacttgt gactccgtct ttttccataa accattgcat agaaattaac aagacaactt
    13441 tcttattatc aatattagga ttcatacttc ctaatattgc agcatcttgt aatgtctgag
    13501 tataggctct cccaattgca aacaccttat ttttactcct atttgtatta aaatagtact
    13561 ttggatgttg ttttgttgaa ttactaagtt ctgatgaccc caaaatcata atatcctttt
    13621 ctcttaaaaa atgattattt gctgtaaccc ctttatcttt tatatcactt aaagtatcat
    13681 ccataattgg aagtagattt ttctccctca aaagttcatc agttttagaa tcaagaaatt
    13741 tgtctaaccc aaataaaaat actacgccta ttataaaagg tgtaataaag tatatcaatt
    13801 ttctcatatt ttctctcctc taaaaatatt caaatttatg ttattattaa tcatattagt
    13861 tttgatatgt atattcaact acaaaattgt aataaaatcg tcaaaacttt ttttgatact
    13921 gttacaaaat tgtaaaaaaa aagaaaaaaa cgccaaaaat aaatttcttt ttagcacttt
    13981 ttcatactta taatttgaca ttttaacact tggttaagta tacaccttac cttaacttaa
    14041 aattaaattt atttaagaat atatattata aattcttata ttttctaata taaactatat
    14101 actatcagct ggataaacaa tactactttg tcttcttatc tcatccataa tttccattac
    14161 tattattgaa ttttcatgag aatttatatt tgattctatt ttattgttat ttataagact
    14221 tataaactct acaagttcat aatacattcc ttctatttct ctaggctttt cctctgcttt
    14281 ttcttttgga atagatatat cttcttctct tccatctctg tatttaataa tagctttttc
    14341 aaatagatta agcttttcaa ttattatact tccctcttct ccctgtattt cagatggtat
    14401 ataagaatca gctactttag aatactgaat tatcgcatcc atatcatcat attgaaatac
    14461 agctgaacct tctccatcca ctccagtttg aagtatatga gaaactgcct ttacagtttt
    14521 tggagcacca aataaattca ccataggatg tatacaatac actcctatat ccattaaagc
    14581 tccatttgac aactcttttt taaaggcatt ttctataatt ccatttttat atttatcata
    14641 tcttgaagaa tactgacaat agcttccaaa aaatcttctt attttcccta ttttatacaa
    14701 gttttcttta actgctttaa agtttggaac acatgtaatt ctcatagctt ccataagcaa
    14761 tacattatta tctttggctg tttttatcat tgatttgact tcttttaaat tagatgctaa
    14821 cgacttttca caaagcacat gttttttgtg ttttaaacaa gtaatggttt gctttgaatg
    14881 taatgcattt ggagaagcta tgtatacagc atctatatcc ttcgatttag ccatttgttc
    14941 taaatcatca aaaaaaatac ttgctccata tagagttcca aattctctag ctttttctaa
    15001 atttctagaa tatacagcaa ccaattcaaa gtctttaacc ctagatgcag ctcttagaaa
    15061 tatatttgct atattgcttg tcccaacaat accaaatctt attttaccca tcataaatca
    15121 ctccccattt tctacacttt attttaattt taataaaaaa ctttacaact tatagatata
    15181 ttataaaata attttatcaa ataaaaaaga aagctaattt taaatgattt caccatttta
    15241 acatagcttt cttttaacta caatctaaat tcaacttata tttttaaagt tatctttcta
    15301 ctatatcttt atttcccctc aaaaatttta tagtaagaat ttcatccaca aacaatgtta
    15361 ctaaaagtat agttacaaca attggccaat aaggtattat aagactatat aaactatcaa
    15421 ctatatacac ttgaaaatat ctaactccaa taaatgtcaa tatagtccaa tatacagaaa
    15481 actttagaca tactattcct ctactattgt acttataatc tgaataatcc caatagacct
    15541 tattaaaata tactttcata agtattcctg taatatattc tactactgtc ggcactacta
    15601 gacataataa aagcaatgca aatatattat tggggtcaat aaatcttgag acctctatta
    15661 taatcgacat tgcaattgca tacattggtt taaatggacc atttaaaaat ccatcttctt
    15721 gaaaatgccc ttgtttataa taactatata agttttcaat tatccaacct ataaaaccat
    15781 acaagaaaaa attaaataaa aaatataata gagaacctac attatccatg aatattcctc
    15841 ctagctgttt atacttattt tttctcatat tcatggattt attctaaatc aaagaaatta
    15901 tatttaattt atttaattaa tactttatat tatagattgt gacattcttt agagttcaaa
    15961 ctttttaggt attctctaac ttccagtaaa gtttcaagta tcttatgtga ttgtgattta
    16021 atttgctcat ctggctcttt taaatctggt acatttatac atcttatacc accattgtat
    16081 gcagcttcga ctcccatagg cgaatcctct atgacaatac aatttttggg attaacattt
    16141 atctttttag ctgcttttag gaatatctca gggtttggtt tagagtttac aacatcatct
    16201 ccacaaacta tagcatcaaa gtaatctttt aaatttgcct tagctaatct ctttactgct
    16261 ctttctctct ttgttgatgt tgcaacagcc attttgtatc cattttcttt taaaaaagag
    16321 attagttcat tgactcctaa tttgatagga gctcccttcc tctccataaa ttcaatcatg
    16381 tttttagttt tttcgtcata taaatctatt attggtacag aactatcata tatctctgta
    16441 agaccttcta ttattccttt acgatttctt cccataactg atgtataaat ttccttagtc
    16501 atagtatatc catacttttc aaacgtctcc atccaaaatt caagagaaat tctctctgag
    16561 tcaaacaaaa ctccatccat gtcaaaaata attccttcta ctttttgcat acaattcctc
    16621 caatattttt tgatataatt atattacact ttttatgatt agttttacaa ccctcaattt
    16681 atccaattct aatattgtaa aattgtaatt ttcaaaagtt acactttggt ttactttaat
    16741 atttgaacca agcatatagg atatccaccc acctatactg tcaaatccat catgctctat
    16801 atcaagtcca aaatacttat taatatagtt gatagattca gttccatcaa ctaagtaaga
    16861 acctgctcct attttcttaa tatgagtctc ttctttgtca aattcatctt gaatttctcc
    16921 aactatctct tctagtatat cttctattgt taccacacca gatgtaccac catattcatc
    16981 cacaactata gcaagttgaa gtttttcctt tcttattctc tcaagagctt tatctatagt
    17041 taaattttca gatatatata taatatctct caatatttct tctagttcaa ttttattttc
    17101 atttatcttt tgattgtata aatctcttat atgtacaaaa cctaatatat catctttatt
    17161 ttttctgcat actggatatc ttgtaacacc ttcctctttt aaaatagcta atatcttttc
    17221 ttcagaatca ctttcatata tacataccat atcagttcta ggaaccatta tctctctaat
    17281 tttcttttct tcaaattcaa aaatattatc aactaatctt tgctctgatt catctataag
    17341 tccactttta taactttctt ctactaatag tcttatttcg tcacctgtat ggggttcatc
    17401 aacctcatca gcttgtgaat atccaaatgg tttaagaaat aaatcagtac ttaagttgaa
    17461 aaaatatatt attggatatg ttaacttata aaatcctact aataaaaaag atgtactcaa
    17521 cattattttt tctgtattat aaagtgctaa agctttagga accaactctc ctattacaac
    17581 ctccatcatt gttataatta aaaaagaaat ggaaattgat atagtatata taatactttc
    17641 actaagatta aatacactta ctattggtaa aattaactct ttaactgtag attctcccat
    17701 ccaacctaat gcaagtgaac aaagtgttat accaagttga catgcagaaa gataggaatt
    17761 taagttgtct tttattattt tacatctttt tgcattatta ttaccttcag ttatcatggt
    17821 atcaatccta gaactacgaa gttttaccat agcaaattca caagctacaa aaaaagcatt
    17881 tatcaaaaca agcattacta taagtgttac ttttaataac tcccgcataa agtataggtt
    17941 cttataatgt aagaaatcta taagaactca aagttccccc cttatattaa taaatttaat
    18001 ttataatata tattctatca tatttattaa tatatcctac tttattacaa ataattttta
    18061 taaactaaat aacccctttt aatatttatg actggctatt tgataaatat taaaggggtt
    18121 atttagttat cattttttat ttctaaaaat cttttctcaa atgatttagt tatctattta
    18181 tccaattctt tacataattt aatgattaaa aatattaatt ttttcactat aaagtataaa
    18241 tattatctga ttattaaaat aaatattaat actataaata aaaacgaagc atgattttta
    18301 tttaaataaa tctcaaactt cgtctttact tacttagata tttatttact ttctaaatcc
    18361 tattattttt aaaaccttat ctttttaata cttcaccaca tttattctca gtaatctctc
    18421 cattttccaa cgcaattttt ccatttacaa ttacatattc aacaccattg gctagtaatt
    18481 gtggctttga aaaatcaata tcttctgacc tagaataact atagttatcc atgtcaataa
    18541 ctgctatatc agctatcaac ccttctttaa gaatacctct attttttatt ttaaattgcc
    18601 tagctggtaa tgaagtaact cttcttacaa tctcttccaa tctaacacca gactttctgt
    18661 acatattgaa gaaaattgga aaagctcctc ttctttgtag ttcaaaccat gacatatccc
    18721 cacccacact aaacaaacaa tctgaaccaa ccataacaaa tggattatca attattttat
    18781 catcatgtag tttatctggg aaatcagctc tatacattcc tcctaaacaa aagataagtt
    18841 cttcgtctcc atctaaaagt aaatcgagta ataacttgtc ttcatctatt cctttttgtg
    18901 tagctatttc acgtattgat ttcttttcca tattaatatc tttagtgttt agaagtatta
    18961 tactccccct atctccaagt ataaatgctt cttttaatat aagtgttctt ccctcttcat
    19021 ttctaagagc atccattacc ccatttatac ctaattcaaa tagactagat gatattgcca
    19081 taataaattg aattactctt tttttcttca tacaatgccc agaacttctt ggtactgtgt
    19141 caaccattat ttcaacacca ttatacctag cctttaatat ctcatcaaat gcttctattt
    19201 caactggact taagtgagat acttgaaccc tagcaccact tttttgccca acctctatgg
    19261 cttcttttac tgcattatat cttcctatgt aatctctaat atgagatgta tatataccat
    19321 catgctcttt aatctgcctt gcaatatcaa caagttcatc aatatcagca tacttacttg
    19381 gtatatatgc caatccagta gatattccaa aagcaccact tttcattcca tcatttatta
    19441 tatctgtaat ttcatttttt tccttttctg taggaggtct atccttagaa cctcccataa
    19501 cactccatct tatagtccca tgccccaaca agaatcccat attgacagaa ataccacttt
    19561 tagatataag gtcacaatat tctgtaaaat tattccaaca tttattttta tctataagat
    19621 actttttttc ttcttcaagt aatagaccat ttttatacat atattcatat acattttcag
    19681 aagaatatgg tgttatagaa tgtccacaat taccatttat agttgttgta actccttgtt
    19741 ttagaaactt ttcaaattta ccatctagta ttgctactat ttcttcatga acatgagggt
    19801 caataaatcc aggtgttata acttttccac aacaatcaat ctctttattt gtattgctat
    19861 ttatcttaga atctattttt gctattaagt tgtctttaat tgctatatct ccataaaaac
    19921 caggattacc tgtaccacct attataaatc cattttttaa gattaaatca tatatcaaaa
    19981 tattccctcc ttaaaaacca atttttaaat aatcttaatg taatttttat atttgaaaat
    20041 caaattatta tatttcatat ttgaaaattc actattattt cattgtaatt agtcactaag
    20101 cttatcttta tacatagctc ttgcaattaa tatagtacca aatacacata taagaactac
    20161 tagagactgt ggtattatac cttgctttga taatataata gtcaatacag atattacaat
    20221 tgctactagg cctaattttt tatcttgtaa aaatacttgt ccaaaaactg acccaaatag
    20281 agctggtaaa attaagttta atttttctac tacttcagct ggaattttag atagtacgct
    20341 tcctcctaaa attaccccta tagttagtat acttatattc actaaaatag atacagctat
    20401 tcctattgta gatattatcg agccttcttc tgtacctgat tcaacttctg ctgccttttg
    20461 agctgctatt gaacatggta gcctcatatt agatatatta ccagaaagaa aactcatata
    20521 agttcctggg atacctaaga ttggtgaata agaaactggt tctattatgt aatttggagc
    20581 actcatactg acaattgcca ttgtacttgc taataatgct gatattggag gcataatacc
    20641 aaatccaaag ctcaaaatta ttccaggaac taatgctgct ataatcccca aagatagtgt
    20701 tagtcttcca taccttatca taaaaggtaa atattcttca ttgtatatag attttacttt
    20761 actcataaaa ctcccccctt atattactga agctattatc attccgctaa acattgatat
    20821 tgtaagcccc cactctctta accattttat atttttcttc ttttcaagca tacacaatat
    20881 taccattata gccattccac ttatacatgc aattgttcca ttatcaaaac gcaacaccct
    20941 atcagcattt agatatgcaa atgaacccaa cattgctcct agagatatta ttggtataaa
    21001 tgatttcttg ccactagata taacattggt tatcttatct agtttatgtc caaatagtaa
    21061 tgtgaatatt atccatccta aagaacctaa tatcatagtc caaactgcat ttgtaaatgc
    21121 aagttttgtc atttcaggag aacctaaagt tatacctaat gcatctgttc caaatccggc
    21181 tgacattgat tcaaaaatta cagaacctat aaatgataat ctcatccatg ctataggccc
    21241 acccatagtt actataagcg ataccattcc agcaaatata gtcattgaag gcccaatagc
    21301 tgaaatagca ctactcttaa cagctgactt tatttgttta tctgttattc ccattgtttt
    21361 accaactttg caagatttta tagcaaatat aagggattgt accactacta ttgatactgc
    21421 tactgtactc gctatccaca aaagtggatg attagctaat tttaaataat ccatttataa
    21481 aacctcccta taaatcctca aattttatta atatttctaa tatatctatt tccaaacctg
    21541 ttttagtact ctaagtatat ttccacctag taccttctct atttcctcat cactatatcc
    21601 atgctttact aaatatctaa caatgttttt tgaagtttct gttgggttct caagaccttt
    21661 tacatattct actttgggat gttcttttgt tcctgttatt ttttttatat caaatttagc
    21721 agctagtact gagtgtaatt ttacatggtc gccatatact gtatctggtc caaaagctac
    21781 atggtctatt cctactaaat ttttaatata ctcaaaatgt tccataaatg actctattga
    21841 atgttcttta ttcttctctg ttattgtagt atgaggtgct gcttctacac ctataacccc
    21901 tcctttttta gcacatgcta ttaatacttc atctggagtt agtctctttg tgttccataa
    21961 acttcttgcc ccaacatggc ttaatattat tgggtccttg ctatattcta ttacatcaag
    22021 tgtagtttga actccaacgt gagagcaatc tattgccatt ccaattttat tcattctttc
    22081 aacagctttt ttcccaaaat ttgtaagacc tccatctttc tcttctttta gtcctgaacc
    22141 taatgcattg gattcgctgt atgttatacc cattaacctt ataccaaatc catacagtat
    22201 atctattcta tcaagttcat tttctattgg tgctgcacct tctaatgttg gtatcaatgc
    22261 tactttacct tcacttttag ctctgtatat atcttctact ttttcacact ttatgacaaa
    22321 atcttgatga gctaaatcac ataatctcat gcctaaatct attaatatat catcccattt
    22381 ccaccctgaa tttgatgtta tcgtacatac accatccatt aaattatcaa atatagcatc
    22441 ataatatcct cttgataatg cttcataagc acatctttgt ttaccttccc tattatattc
    22501 aaatatatct ctttctaggt gttctggaaa taatactgga tgttcatgta atgatataaa
    22561 tattttttca tccactatac ttttaacctt agcttcttga atatcatcta attcaataag
    22621 gtattcttta actcttttat ctcctttaca catctcaata gatgaaatat cttttccttt
    22681 ttctaaataa ctataggctt catatccttt gtaagctgac ttcataacaa catcctccaa
    22741 gttctacaat tttttatatt tttcctatgt agcaattgat gtgccaaaat ataaatttta
    22801 attgtataat aaatcttgca aaaaataaaa acagtagtaa ggttgatatt tactactgtt
    22861 ttattaaaaa taatcttctt agattattta ttcaatattt ttgaattatc ttaatcttaa
    22921 agataatttt atttatttct atccattata gggatttttt tagggtgttt tgccttttta
    22981 gtcccctata tcactccctt aatttttaca tatgatataa agttctttcc ttcatcagtt
    23041 ataaaactac cttgtttttt tactccagat aaaagtaaac catggctctt taacttatct
    23101 attctttttc taatttggtc tacacttaaa actatatctt tttcttttaa aatctcttgc
    23161 agtttatttc ttcctaataa tatattttta ttattccaag tttctatact ggtcaatata
    23221 catatacttt cctcaaagaa attagattgt ttgaaatcta agataattga atcgaaattt
    23281 tcattaacta aagtattgtt tttttcaaat ctaaattgtt ctggcaaatg ctcgtaatct
    23341 actctatctt cctcaactat agtatcaata taataaatta aatttttaag ctctcttaca
    23401 tttcctggcc attcatacaa tgttaaagct tgcattgatt cctctgtaaa acatttgttg
    23461 ctatttattt catctaaaaa atacttactt ataagaggta tatcttcctt acgttctcta
    23521 agtctaggta tctctatatg taatacatta atcctataat acaaatcttc tctaaaagaa
    23581 ccttctttaa tttttttctt taaatcttta tttgttgcag ctataattct aacatctatt
    23641 ggtataattt tacttccgcc cattctcata acttcttttt cctgtaatac tcttagcaat
    23701 ctctgctgta catctaaaga tatatcacct atctcatcta aaaaaattgt tcctgtatgt
    23761 gctctttcaa atatacccat ttttccacct tttattgctc ctgtaaaact tccttcatca
    23821 tagccaaaaa gttcactttc aataagtgta tctgacaaag aagatagatt aactgcaaca
    23881 aatggcttgt tttttcttag agattcatta tgtattgcct gggcaaatat ttcttttcct
    23941 gttccatttt ctccaagtat caacacagaa aagtctgtag ttgctatttt tcttgcaata
    24001 ttaatttttt cttttattat tttactctca cctacaacac tctcaaatgt atactttgat
    24061 acaaaacctt ttgactgaag tttattttga atcttgtcct caagcacttg aattgcactt
    24121 atatttttaa tgcttataat actctttatt cgttcattat tttcataaac atttacttta
    24181 ttaattatca gttttttgtt atttaaattt actacttcat cattaacttc atcttcttga
    24241 aaaaatattt ttttgacaac cttatcacta aataagtcca taaagttatt agatataatt
    24301 tcattttggt ctattccaac tagattagaa aatactttgt tgcaatatat aaattttcct
    24361 aatttatcaa cagaagcaat accttcatct ataatctcca aaaaactctt catggtttta
    24421 ttcatagttg tataatacct atatccactt actgtatctt cattgtattt ttccttaata
    24481 atatgtagtt tatctataga tatatttagt tttgtaaaga tttctataac tgtatttata
    24541 tcaattacct tatcacctat gtcaattatt tgctttatgt tttgtggtat gctgtttctg
    24601 cttcccgtaa taattccaat ctcacattta gacttatcac aacctggata atatggtatt
    24661 agattaatat gaattaatcc tagttttcta attatcttgg cagtttcatc tgctgactct
    24721 ttataagcat ctataaccat aacatcagaa tcattttcta tacttattat ctgatttata
    24781 ttttcaatat ttatagtacg atgtactatt acaataggta tatttttatc aatattatta
    24841 tgtatatatt cctttatatc attgtctgaa catactacaa gctcatagct tgacagttca
    24901 atatttatcc tatttacata taccaagtta tctatattac aaaattctcc aaatatattt
    24961 tccaattgtt ttgatatatc tatatttatt ttgtcatcag ttgatacaaa taaaatatta
    25021 tgttttttca tagtcccacc tcctgtttaa cgagtataga aagtaataat ttggtctatt
    25081 gtagcacttt ttaaaaaagt tttaaatctt aaaaatcaaa ttttgtacca tttactatta
    25141 ttttccataa taaaataatg taagctatct tttttcacat ataatttaat ttctaaaacg
    25201 gttagtttga cattattgga atataaacaa aaatttaata taaattcgtt aatatattaa
    25261 tacattttta agaatgacaa ggaataaatt ataacaaatt ttaggaatat ataataatag
    25321 ggtctcagaa cttagttaca agttctaaaa ccctatttgt ttagtgtaaa aaacatcata
    25381 aaatttagct taaatctttt cacataatat tactcttttt ctaagtgata ataaaatata
    25441 tctattcctc taaaaactgt aaaaattcct gattaaatag tttaagctca tcatagaata
    25501 tagcatgacc actatactca aaagtatata aaattgaatc ttctattttt tcattcataa
    25561 aaactgcaaa ttcatacgga catatttcat ctaatttccc atgaaatata ccagtaggca
    25621 ctcttacaca ttttaaatca ttaaataatt cttcatctct taaagataca gcagttccaa
    25681 tagtgccaat acctgatgca ctaaaaccaa tatcattaaa ccatcttcta aagctttctg
    25741 gtggatttga tgcaaatact ttttcgccaa aatcggttac catttctggt ctatcagtac
    25801 atgcttgtgc tatcaattta tttacatctt cacgtgtcat accatatgga aattctggag
    25861 gtctttgtac aaatgatggt gcagcagctg cagctaatac caattttgac actccatatc
    25921 cattaaataa actcatatac ctaagtacga ttgctcctcc cattgaaaaa ccaacaaggg
    25981 taaagtcttt taatccaata gcatgtacca ccttataaat atcgtctgct aattggctat
    26041 aagtatatcc tcctgatgtt gcatcagatt taccaaaacc tcttaaatca attgaaactg
    26101 ttctatatcc gagttttggc aatatatttg tttgatattc aaatatctta tgtcctaagg
    26161 gccaaccatg tataaaaagt acagtttttt tagcactcgg attcaaatca tatactgcta
    26221 tgtttatatc attcacatat acataataca tatcaacagc aactcccttc aagaaaatgt
    26281 atgatggtta ttttatatat atgaataaaa tgatgaataa ctaaattttc aaatttattt
    26341 acaatacttc tctattgcct tttcaaataa acttacatga agtttttcat ctaggattat
    26401 tctatttaat atagctttta tataagggtc ttcaataaga cttatatctt tataataatt
    26461 attaatagcc acatgctcca aatgcaaatc tagttttaat ctgttacaaa tagaatttcc
    26521 ataacataca aaaccaccat tccaatatgc accacaagaa cttgtagaac ctctatatac
    26581 aggatttcca cccaataatc ttatagtctt tgctaatatc tccatatgta acatttctgt
    26641 tatagatata tttatccaca tttcacttaa ctctctatgc gtttcatcta aatcaaaatt
    26701 atggtataga tattgattta cactcgtaaa ttcacttgat actccggcat agtcatccat
    26761 caataattct gcatagtact tatttggacc taaaactttt atctctggat atggctcatc
    26821 acttgaatat ccatttcgct tgtgcatgtc ataatgtgca tcactcataa tatctcctcc
    26881 tttaaattta atgtatgata ttaattgtat gcatcacaac tttttcatat aacttagata
    26941 ataaaatcta cttaaataat aaaaagccat attaattaaa attaacatga ccttttatcc
    27001 acaaaattac tttttaatta ttcatctttt ccagctactg atagctcttt aataacaact
    27061 gaaggtgaac ctatactact cattggaaat tctaaatctt cccctatagc ttcaatatct
    27121 ttcaacaggt caaaatagtt tcctgctact gtaatttgct ctactggaaa tacttttttt
    27181 ccttctttga tataaaagcc tttggcagct aaagaaaagt ctcctgttac tgaatttgca
    27241 cctgaatgaa gtcctgcaaa gctagtaacc atcaatcctt catctatatc ctttataatt
    27301 tcatctaaag atttatctcc tttctctata taaaaattag ttggatatat gcttataggt
    27361 gatgaatatg aagattttat tccatttcct gtagtcttta cacctgcctt attagctgtt
    27421 tttaagttat gaagtaaagt tataagcttt ccatttaaaa caacttcttt tttaaatgta
    27481 gctactcctt catcatcaaa aggagtagat gccataccat tttctaaaag tgggtcatca
    27541 actattgtaa ctataggaga tgctatcatg tctccttctc tatttttcaa taaagataat
    27601 cctttttgag ctgaatctgc attaaatatc ccagaaaatg tgctaagcaa agataccata
    27661 gcttcattaa gcaatatagt tttatattta cctgatggaa tactcttacc acctacttta
    27721 gataaagcat tatcaacacc aagttttgct atctcacaag gttttatttc ttcaatagaa
    27781 tttgctatat tatatcctat tccatcatat ttttgtccat tgtcttctat gataggaact
    27841 acaaacccta taaacatatt agttttattg cttaaattaa gacctttagt attagatata
    27901 ccattacttg atacagaata agatatagta catttagata aatttaccac cttatctgaa
    27961 taagctttag tctctctttc catttctaaa gctaaatcaa tcaacttatc tgcctctaaa
    28021 ttttctagct ctttagaata agtctttaca tcattatagt gcttatctcc ttcatatata
    28081 aattgtacat cttcattttc aatagtgttt actccatctt ttacattttt tatcaacata
    28141 tcaacagctt tatcatctaa aatttctgta taagaataac ctatctttcc atttacctta
    28201 cctctaaaag ataaaccaaa agatttatca agattgtatt tctcaacttc tccttcatat
    28261 atatttatac ttaaattttc tcctgtataa taatatattt cacactcttc aaaaccttca
    28321 cttagagcct ttttaaataa tatatcttta aaatttttaa gttccacaat tatccctcct
    28381 atcttccacc tacagtaatt tcttttaccc ttatcatagg ttggcctaca ttagttggta
    28441 tacttcctga tgatgagcca cacatcccct gagcttgttt tacgttattc ccaaccatat
    28501 ctatattcat aagtatatcg ctaccttttc caattaaact agcacctcta actggctctt
    28561 gtatttctcc attttttatt aagtaacctt ctgaaactgc aaaattaaac tctcctgtta
    28621 caggatttac agaaccacca cccatttttt tagcataaag tccatctggt attgacttta
    28681 taatatcttc tggtttatcc tctccagcag caatataagt gttagtcatc cttgaagttg
    28741 gtgcaaattt gtagttctgt ctacgagaac ttcctgtagg attcattccc attcgtctac
    28801 cattaagttt atctatcata taagacttca aaattccatt ttcaatcagt atattctttt
    28861 gagttggatt tccttcatca tctatattca tagaacccca gtagtttggt attgtaccat
    28921 catctatagc tgtaatcttt gtggaagcaa tctgttgacc taatttatct gcaaatacag
    28981 agttcccctt tgcaactgat gtagcttcca aagaatgtcc acaagcttca tgaaatatta
    29041 ctccaccaaa tccattatct attgctacca tcatattacc tgcaggacaa tttttagcat
    29101 gcaacatagt atgcgctact ctagctgatt cttttgcata atattctggg tctatttctt
    29161 caaatatttc aaacccttta caacctccag gaccttcaaa tccagtttga ttttcattgc
    29221 ccttagatgc aattgaactt atccccagtc tggttcttat tcttctatcc tcaacatata
    29281 taccttcagt gtttgcaatt aatatttttt ggtctttatc caaatagcta acacttactt
    29341 gagatatatc attactaaat tcttttgcac tcttatatgc aaccttcata acacttactt
    29401 tatctctatt tcctattgaa tttggataaa tttttatatt atgtatgtta tttattttaa
    29461 tactgtcatt taatattaca ctttttccat cttccaattt tcctaaagca acagcagctt
    29521 tgtatgctac atccaacaga ctatttaatg aattgtcact tgtataagca tatatacttt
    29581 tcaatccttt aaatattctt attcctatgc cataatttct tccacctatt gcattttcta
    29641 ctttattatc tattaaacta atagaattat ttatagtgtc ttcttcaaaa atctcagcaa
    29701 aatctcctcc agtaattaga cacttttcta ataccttaga agctatattt tttgaaagca
    29761 taaatttacc tcctacttta aatttttaat taacttttat tcaatctttt tacttgttaa
    29821 caaacattat atactatatt acttaaaata aggtaattat gaatatatta tacatatgtt
    29881 ttatactaaa atgttatcac attttatcta tacataccca tataactaga taattaaaat
    29941 gtataatatt atttgctata gaaaataata cactcataca taataaattt taggagtgaa
    30001 gtatatgaat caagaatatg taaaaggaaa tcctattttt actaatatta ataaagttcc
    30061 aagacaattt ccatacctta ctgatgatat tgatactgat gttataattg ttggtggtgg
    30121 cgtaactgga tgtatctgtg cttactatct agctaaaaac aatataaagt ctgtcattct
    30181 tgaaaaagga aggattgcac atggaagcac tagcgtgaca acttctctat tacagtatga
    30241 attagatgac aatttgatag atttaacaga ggttatgact ctaaaagatg ctctaaaagc
    30301 atataacctt tgtacttcag cacttgaaga attagataca tttatagagc tatatggaaa
    30361 taagtgtgat tatgccaaaa gagatactct actttatact gccaacaaac ttgaagttaa
    30421 ggctatcaaa gaagaatata acttaagaaa agaaaatgga tttgatgttg aatatattga
    30481 tgaatccaca aaccctttta gctttgattt aaaatcagga ttgatagcaa agaatggagg
    30541 acgagaatta gacccatata aatatagtca ccatttgatt gatgtaagtt taaaaaatgg
    30601 tcttcaagta tatgaaaata cagaagtaaa aaaagtagat ttatcaaatg ataaggttac
    30661 tgctgaagta tcgtatggat acaaagttca tggtaaaaaa ttaattgtag ctacagggta
    30721 caatacaagt ttatttacaa aaagaaattt tgcaactaag tcaaatactt ttaatatagc
    30781 aacgaaacca cttaaaaaca tagcttcttg gaaaaacaac attttaatta gagataactg
    30841 tgacccatat aattatctaa gaactactaa agacaatcga ttaattatag gtggtgaaga
    30901 tgtaagtttt gatgatatag aaaatgaaac tttagccaat gaaaagtacg atattttaga
    30961 acaaagattg aaaagtatgt ttaaggatat aaaagacatt gaagttgaat ataaatattg
    31021 tggttgtttt gcttcaactt tagataattt aggttttatt ggtccagata ataaaaataa
    31081 caatctatgg tattgcttag gttatggagc aaatggtata ctctttgcaa tattaggagg
    31141 tataatgtta agtgaattat atcttggcaa acaaaataaa aatatgaaat tatttaaagt
    31201 agatagattt gataaataga tattatatat taaaagactg taaaaagatg aaactttata
    31261 attctaaaag ttaactttga tacagtcttt tataatgttc cttttaaaaa tactttattt
    31321 ataattaaat ataaagtaaa atgttaaaat ataactgata aaatttagtc ataagataat
    31381 atttaagttt atttgatgaa atatggagga attattatga aggaaatact cgttattgct
    31441 cccactaaag gaacctatga aaaatcaatt cacatagtta aaaaaaacaa atatacaaat
    31501 attgatgttg tatttggcaa tctaaaagaa ggtatacctc ttgctgaaaa aagtattaat
    31561 catggtacta gaattatcat aagccgaggt ggaacttaca atatgttaaa agctacttac
    31621 aatataccta tagttgaaat taaagtagat gcttatgata ttataaaaag ttacaaagag
    31681 gtaaaaaatt ccaacgaacc atttggaata attggattta ataatgtcat ctacggtttc
    31741 gatattatag aagaaatatt gaataagaaa ataactatga ttgaaattga aaaagaagaa
    31801 gaaatttatg atgctataga aaagtacaga aagaaaggta taaacacata tataggagat
    31861 accactgttg cacatattgt taagagactt aattgtaagg gaatattaat cgaatctaga
    31921 gaagaaaaca tacttagagc cattcaacaa gcagaacaaa tactcgaagc aaccaaggat
    31981 gaacaaaaaa gaagattaca gatagaagct atgacagatt ttgtacatga tggaataata
    32041 acagttgata aaaattttaa aataacatta tttaataaga gtgctgaaaa aatatttggt
    32101 ataaaaaaag aaaatgcact ttatcataat gttgtagatg ttatcccaaa cacagttctt
    32161 ccacaagtag ttgagactat ggaacctcaa attggtgaaa tacaaaatat agggagtaca
    32221 aaaattatta caaatagagt tccaatcatt gttgatggtc agattcaagg agctgtttca
    32281 acatttcaag atagcaagga gataagttct tttgaacata caataagaag gagcttatta
    32341 agcaaaggtc tatctgctaa atacactttt aatgatataa ttcatgctag tgaaaagaca
    32401 aaagattgta tagaacttgc aaaaaaatat gcttgttatg atactcctat gattattact
    32461 ggagaatctg gagttggtaa agaactattt tgtcaaagta tacataacta tagtaagcgt
    32521 aaaaatgcac cttttgttgc tgtaaattgt gctgccatac ctccatctct aatagagagt
    32581 gagttttttg gttatgagga aggttcattt acaggtgcta gaaaaaaagg taaagcaggt
    32641 gtattcgaac tagcacatga aggcacaatc ttcctagatg aaataagtga aatccctctt
    32701 gaacttcaag gtcgattatt gagggtattg caggaaaaac aagttatgag aattggtggt
    32761 gataaagtta ttcctataga tgtaaaaatt atctgtgctt caaataaaga cttaaaaaat
    32821 atgatgcgca atggtttatt tagacaagac ctttatttta gaataaatat actaacatta
    32881 tatcttcctt cattaaaaga aagaaaagaa gatattataa aattatcaga attttttata
    32941 cataaatatt caagcaaata caataaaacg cctttaatta tcactccaaa tattaaaaag
    33001 gcacttcttg aacgagaata tgaaggtaat ataagggaat tggaaggtat gatggaaagg
    33061 gctgttattg tggaatcatt tgactcgatt ttattagaaa agcttagcaa tgaatataca
    33121 aataatatag ttgataaaga taatagtgat tttgaatgca gtaatttaga tttaaaaact
    33181 ttagaaaaaa aatatatcta taaaatttat aacgaaaata atagaaatac tacaaaaacc
    33241 tgtgaaattc taaagataac tcgctccact ctatggcgta agttaaaaga aatagaagat
    33301 gatgtttaaa aataatacat atctatatat atcaaaacgt tttatgttta attttaatac
    33361 ataagacgtt tatttgtttt caataaaaaa tacaattata tgataaaaag aatctttgta
    33421 agtgaaattc tcgtcaatca tacttttaat tataagtaat ctacagcatt tcaatcgatt
    33481 attaaaaata atcataataa ctaaagatac aaactctata tattggcata gtaattgctt
    33541 ttataatacc tatataaaaa tgaaagggag tgatactaga tgcacatctt aaaatcagtg
    33601 aaaaagatac caggaggact tatggtctgt cccttgttat ttggagcact atttaataca
    33661 gtatgtccac aagctcttga aattggagga ttcacaacat ccttatttaa aactggtgca
    33721 atgagtatac ttgctctatt ttgtctatgc aatggagctc aaatcaatat cagacaagct
    33781 ggtaaaccat tgactaaagg tatagtactt acagcaacaa aatttataat tggtgcagta
    33841 ttaggtatta ttgttagtaa atttattgga cctaaaggta taattggtat tacacctctt
    33901 gccatagttg ctaccatgac taattcaaat ggaggtctat ttgctgctct tgcaggtgaa
    33961 tatggagact ccacagatgt tggagcaata tctatcttat ctctaaatga tggtcctttt
    34021 tttacaatgc ttgcttttgg agtaactgga cttgcaaatg tacctatagt agcactattt
    34081 gcagcactta taccaatact acttggattt atacttggta accttgatga agatatacga
    34141 gaatttcttg cacctggtac aacattatta attcctttct tcgcttttcc tttaggagca
    34201 gcattaaact ttaatcaaat tataactgct ggactacctg gagttgtact tggtttagga
    34261 gttactataa taacaggtat gggtgggtac tttgtaatga aacttatacg tgcagaacat
    34321 ccagcagtag gtgcagctac aggtacaaca gccggaaatg cagctggtac tcctgaagct
    34381 ttagcagcta tagaccctac tctagctaca atagcagctg tttcaacagt acaggttgct
    34441 gctgctatta ttgttacagc aatatgttgt ccaatgttag tttcctatct agataaaaga
    34501 gaaaaaaaga aaaaatctat ttttaaaaca aataaaaata ttgaagtaaa ttaatactta
    34561 aggaggtaaa attaatgaat gcaattattc atgcaaatga aaaagataat ctagttacat
    34621 gtgtcagacc tttagttaaa ggtgaaaaag tttcaattga tggaaagtct tacatagtaa
    34681 atgataatat acctgtcttt cataagatgg ctacagaaga tgtaaaaaaa ggtgatgttg
    34741 tatacaaata cggtgaaatt ataggaattg caacagttga cataaaaact ggtgactatg
    34801 ttcatgtaca taacattgaa agtacacgtg gtcgtggaga taaaaaatag gaggaagtta
    34861 aaatgaaatt aatgggttat ttaagagaaa atggtcagtt tggtattcgt aatcatgttt
    34921 taattattcc aacatctgta tgttcaagtg agacagctac aagaatagca tcacttgtgc
    34981 ctggagcaat agcaatccct catcaacatg gatgttgtca aattggttct gatatagagc
    35041 ttacagcaaa gacattaata ggatttggaa aaaatcctaa tgttgccgca gttttagtag
    35101 ttggtcttgg atgtgatggt attcaagcaa aagaattagc atctgaaata gctacaacag
    35161 gaaaaaaggt agattacatt gttatacaag aatgtggcgg aacattaaaa acagtttcta
    35221 agggagctga aatagtaagt aaaatggctc gtgaagtatc aaaagaagta agagttgaat
    35281 ttggtatgag tgaaattaca ttagcacttg aatgtggtgg ttctgaccca acaagtggaa
    35341 tagcttcaaa cccttcaata ggcgttgcat caaaccttct tgtagatgaa ggtggaagta
    35401 gcatactaag tgaaactaca gaagtaattg gtgcagaaca tttgttggct acccgttttg
    35461 aagatgaaga aatgaaagat aagtttctta aatttgtaag tgatgtagaa aaaagagcaa
    35521 ttgctatggg agaagattta cgtagtggtc aacctactcc aggcaataaa actggtggac
    35581 tttcaacaat tgaagaaaag tcacttgggt gtatgtataa ggcaggcaat aagccattca
    35641 aaggtgctct tgaatatgct gatattgtac ctcctgataa aaaaggatta tattttatgg
    35701 atactccagg tcaagatata gattctatca ctggtatggt agcaggtggt gcacaaattg
    35761 taatcttttc aactggtaga ggtactccaa caggaagccc tatttcacca gttattaaaa
    35821 tcactgggaa tagtgataca tacaacaaaa tgccagacaa tatcgatata aatgcaggta
    35881 gaattataac agatggtgct aaaatagcag atataggtca agaaatattt aatgaaataa
    35941 tagaagtctg taatggtaaa cataccaaag ctgaaagtct tggtcataga gaatttggca
    36001 tttacagaat ttcatctaca ttttagattg taacaaaata tcttttataa atataatact
    36061 tatgtagata cataagctaa aagttctttc taatactata aaaattttct atactgaaaa
    36121 ggaactgtat caaaattatt ttgaaaacag ttcttttttt aacctctttt actcaaaata
    36181 ttataaaata agatttaaac aataaacgta tcttgattaa tctctaagaa gacataaata
    36241 aagctaaatg taaagtggga taaataaaat ttaatatctt tgtatgaaaa gatatttggt
    36301 ataatatgtc tgagagaatt cttatttgta aacaaataaa ggatggagga tgaaacaaaa
    36361 gaatgaactt tgaaaacaca aaaaataaaa aaataatagc tactttacta ttgacacttc
    36421 ttttaatcgt gtcattaaca tgtgtcactt ttattatgaa tctaaataaa gtaacacata
    36481 atgaaacaga acgatatctt gtagaagttt cacaaggtat ttccactaca attaatgtga
    36541 aatgtaaaag tatatttagg acactatatt ctatgggaga tacttatttt gagattaatg
    36601 atagtgaaaa agaagtcaat agatacttat caaaaaaatt aaaattattt gaatttgatt
    36661 ggttaggtat tatagattct aatggaaatt ttgtaagtgc ccttaaagat acaggtaatg
    36721 ttaaaggaca agacttttat agaaaagccc ttcaaggcaa agaaacaatt actcctgtta
    36781 aatcacaatc tggtacatat ggaattttat attctgtacc actatacaat ggcgataaaa
    36841 ttgttggagc tgttgctgct tggaatacta ttgatactat gagaaattta ttaagtgtag
    36901 aaagctttca tggtgaagga ttttcccaaa ttatcgacca agatggaaat tttattgtaa
    36961 gttcagcaaa caaaaatgct ccaaaaaatg tggtaaattt ttttgacatg attaaacacc
    37021 aaggaaaaca ggttgataaa aataaacttg aagaaatgaa aataaactta aaaaagaaca
    37081 aaactggttc tatgtattat acattaaatg atggtatttc taaggcaatg cattatcttc
    37141 cattacaaga agaaaactta tacctccttt caattgttcc aacaaaaaca gcaagtgcac
    37201 agtttgattt acttctaaaa aaagcaatct tcatcaatat tttaattgtt atactatttt
    37261 tattgcttat tatattgatt atgtttataa atcaaaagaa cagggcacaa cttataaaaa
    37321 tcgcctatgt agactctgta actaaaggtt acagtaaagc acgttttgat attgaagcta
    37381 aaaaaattat acagtcatct tcatcagaaa gctattcatt ggtatctata aatattcaga
    37441 aattcaaact tattaatgat tcttttggta gtgaagcagg agataaaaca ttaaagtaca
    37501 tacatgatgt cattagtaat tatctagaac caggagaatt gctttgtcga gtttctaatg
    37561 accagtttaa tgtacttatt aaaactattc ctcaaaaaga aattcttaag tacatagaaa
    37621 atatcaccaa tgatattaat agtttcaata aaaatgaaga atgtaaatat tttatagctc
    37681 tatctatagg tgtatataaa attgatgacc caaatctacc tttagtttca attcaagacc
    37741 gtgcaaatgt tgcacgaaaa aataataaag gaaacattgg aaatcatcca ctttatacat
    37801 gtgtttttta tagtgattta gagcgtttaa aaatgattaa agaaaaagaa atggaaaata
    37861 gaatggagga agcattaaat aataacgaat ttatagtata tcttcagcct aaaataagcc
    37921 tcaaagataa tactattgta ggtgcagaag cattagtaag atggcaagac ccaaataaag
    37981 gtcttatacc accagacgag tttattcctt tctttgaaaa aaatggtttt attacaaaac
    38041 tcgaccacta tgtttttgaa aaagtatgca gtatgataag aaaatggata gataatggag
    38101 aaactccaat acctgtttca gttaattttt cacgtgctaa tttaaataat atagattttt
    38161 tagaaaaata cagaatgctt cgtaataaat acaacattcc tgctaagctc ttagaaattg
    38221 aattaactga aacacttgta tttgaaaatc tccaaaaatt aattaatata atagaccaaa
    38281 tccatgaaga aggttttcaa tgttcactag atgactttgg aagtggatac tcttcactca
    38341 atatgttaaa agaaataaaa gttgacacat taaaacttga ccgtgctttt tttagctcac
    38401 ctaatgcaga taatattgct gaaaatcatg taatagaatc tatagttaat ttggcaaaaa
    38461 aattaaatat gaatagtata tcagaaggtg ttgaaacaat cttgcaaatg gattatctta
    38521 aaaaaattaa ttgtgatatg atacaaggat atgttttttc aaaacctctg cctcaagaaa
    38581 tatttgagaa aatgactttt ggaaaaaaac tagaagaaac cagtatggat agttccgttt
    38641 aaataaaata aaaaacttgt ctctataaaa taattttaat cattttataa agacaagttt
    38701 ttttattttc caatttaatt ttattttcta aatttcttat actcggcttg ttttaaattc
    38761 cctttttaag aataataata cgattatcat agcaataaaa tctgcaactg gctgtgccaa
    38821 ccaaacacca tctaaattaa attgaattgg aagtacaatg attaatggta taaataaaat
    38881 aaattgtctt aaaagagtta aaaacattga atgtttaact ttacttatac actgaaaata
    38941 cacaggtcct acaatagaaa tccccattat aggcaatgta attagattta ggcttatacc
    39001 tcttactgct atatccatca aatctgaatc tttattaaat atacctacaa atacttctgg
    39061 gaaaacttgt actactaaaa atccaaatga caatatgaca actgacacta tgatagataa
    39121 tattaaagtt ttctttgcac gctcatattg ttttgcacca taattgtatg atattatagt
    39181 ttgcatacct tgattcaatc caaacacagg catcataaac atcaataaaa tagatgtaag
    39241 tgctgtcata gctccaatag ctaaatctcc accataaacc tttagagaat tatttgtaaa
    39301 catgtgagtt atacttatag ctacttccat agcaaaaggt gtcattccta ttgcaaatac
    39361 cttctttact atatctttat tcaattttaa gttacatttt tttagtttta aattggattt
    39421 ccctcttata aaataatgta ttacccaaaa gaatactact atttgacaga gtactgttgc
    39481 tatagctgct ccttttatac ctaaattaaa tacaaatata aatatagggt ccaatatcag
    39541 gtttaataca catcctacaa tcattgtcct tgctgctaat tttggattac cttctgccct
    39601 aattgcatta ttaagagaaa atgcaactaa attaaatata cttccaacca atatgacact
    39661 catatagtct tttgcataaa atattgaatc tttacttgca cccaaaataa aaagtatatc
    39721 ttctaagaaa actaatccta atatagttat aattaaagag ataattatag ataatgtcaa
    39781 agcattacct aatatcttct ctgcttcttc tctatttctt tcacctaact ttatagacaa
    39841 tcttgtgcta gcacctactg ataccaacat tccaaaagct attattagag taaatactgg
    39901 catagttaca ccaagacctg caatagctat agagccaata ccttccatag aaccaataaa
    39961 agctctatct actacattat aaagtgaagt taccatcatt gctattattg caggtatcga
    40021 atacctaaat aataaaatcc atattttttc atgtcttaat gcttcttgat tctccatgtt
    40081 gtcaccattt acaccatgtc ataagttata caaattggtt tatttgttct tgggtctgtt
    40141 gctatttctg catcaatatt aaataattct tttagattat ccttcgtcat aacatcatat
    40201 gcatcacctt cacaaactat tttacctttc ttaataccta tcatgtggtc agcaaatctt
    40261 gctgcattat taagttcatg tattaccatg acaatagttc ttccttgttt tttatttagt
    40321 tcatctaata attttagtat ctccaattga tgtgccaaat ctaaatatgt tgtaggctca
    40381 tccagtaata atatatcagt ttcttgtgcc aaagccatag ctatccatgc cctttgtctt
    40441 tgtcctcctg ataaatcttc catatttcta tccttaaact ctgcaattcc agtaacttct
    40501 aaactccaat ttacaatctc tctgtcttct ttactagaat taccaaatcc acttttatga
    40561 ggaaatcttc cataagaaat taattctgaa acggtaagac cactaggagc ttgagggctt
    40621 tgtggaagta cagccatatc ttttgctatt tctttagact tctttttgta gatgtcctct
    40681 ccatttatat atattttccc actattaggg ctaataattc tacctatagt tttaagtatt
    40741 gtcgattttc cacaaccatt agcccctatg atggtagtta tttttccttt tggtatattt
    40801 aaatttaaat tttttataat atctgtgttt ccatatgata catttagatt cttagtattt
    40861 atacagttca taacaacacc ctttcctaat catttaccaa cattaaataa ataaagtacg
    40921 gcactcctat tatagaaact actataccta ctggtatttc tataggtgct agaagatttc
    40981 ttgatatagt atctgctatt aaaagtaata atgttcctat caatgctgat actggtatta
    41041 atcttttatg ctttggtcct attatttttc tacctatatg tggagctatt aaacctaaaa
    41101 atgaaatact acctgctact gctgtcgata caccagataa aattactgca tatattataa
    41161 gtcttcttct ttgtttttct acatctacac caagacttat agatacttca tcacccaaat
    41221 ttaaaacatc tatatattta gatttataaa tagttatacc cataaaaatc actataaatg
    41281 gaagcacagc tattacatat ttccaactgg ttccccaaat gcttcctgat gtccatgcca
    41341 taaccctatt aaattcttgt gttgtaaatt tcaattggaa tatcgtcaaa attgaagtaa
    41401 aagctatgtt tacacctata ccaataagta gtaatcttga cgaatttata ccacctttcc
    41461 atgcaagaga atatataaga aatgctccaa ataaagctcc tataagtgca acaataggca
    41521 ttgtaaatac agtcaagtta ctcatacctt catatacatt accattcata aggtatatat
    41581 aaactactac aaacaatgca gcacctgaat ttattcccaa aattccagaa tcagctaaat
    41641 cattctttgt aactccttga agtattacac ctgctgttga taatgcagaa ccaaccaaaa
    41701 gtgctattac tattcttggt aatcttaatt taaatattgc aatttcatga ttacgctcac
    41761 cctgccctaa aaaagtcttt ataacatcca ttggttctat agaaaatgag cccatattta
    41821 aactcaataa aaaagtgata aaaataagca ccattaaaat tgatataaca agtaatgttt
    41881 tagaatttat ttttataata tttttcatag tacctatttc tccccctttc taacaaggta
    41941 tataaatatt ggcactccta taagtgctgt tatagaacct actggtgtct catatggagg
    42001 atttatcatc ctacttaata tatctgtata tacaagcaat acagctccta atactaaaga
    42061 gctcggtatt atatacttat aatctgaacc tactattcct ttagctattt gaggaactat
    42121 aagacctata aaaataatat taccagacac tgacacagat gctccagtca ttaatattac
    42181 aagtactata catataaatc taacaaagtt agttttttgt cctaatccta ttgccacttc
    42241 ttctccaaga cttaatatag ttattcttgg agccataata atagctaaaa taaatccaat
    42301 accaccagct acaaatagca attttatacc ttcccacttc actcctacaa gccctcctga
    42361 tatccagaaa cttaattgtt gagataggtt aaaatacata gatattgctg atgccaaaga
    42421 aattagcaat gtaccaagag cgactcctgc aagagctaac tttacattat taactcttcc
    42481 tcttgattta gaacttataa aatatataaa taatccactt atacttgctc caataaatgc
    42541 aaacatcatt acactaaaac ttccatgtat aagttgagga agtttttttt gaagtaccag
    42601 tgatatcgaa attgcgaatg tagctccttg tgtaattcct ataacagatg gttcagctat
    42661 aggatttctc gttattcctt gcattattgc cccagaaact gctaaaaatc ctccaacaag
    42721 tgcagctgct attgcccttg gaagtctaac atcctttact attctcatat ttattccatc
    42781 ttccattttt atcaaactat ctataacagt tgatagattc atatcttttg ccccaatagt
    42841 tatggataat acaattccaa atacaagtac aactatactt actaatatca acaaataagt
    42901 tatatttttt ttactcagct tagtcaatta tcctacctcc ttttcccttt taatcgtcag
    42961 tataatatca tctattaagt attttaattt taagatttat taaaaatagg tgtgaaaaat
    43021 gaatatcatt atctcacacc tactaagtta agaattactt tgttaattca tttttaactg
    43081 attctaataa tagctctttt ccaattggat tgtatgattg actgaaaaat ggagcagcat
    43141 caagtattgt tacattacca tcttttactg ctcttatttg agcccagata gcactatttt
    43201 ctaaatcttt tttatctgct tctgtagcaa ttacaactat atggtctgca tctatatctg
    43261 ttaaaccttc catacttacc attggtagtg ttattccatc ctgttttggc atattcttag
    43321 gtctagctaa tttcatatca tcattgatta gagtacctat tccaccatca ctaaatacca
    43381 taaattgacc tgaacttgcc aacactggta aataagtttt ttctccattc ttttctataa
    43441 cttccttacc taactttgta gctttttcat catacttctg tagccaactt ttagcttctt
    43501 cttctttgtc aaataacttt gaaacatcag ttaacttgct tctccagtca tttgcataat
    43561 ctttcatcat aactacagga gctatttctt ttaattggtc atatattttt tcttgtcttt
    43621 gactcattat tattaaatca ggatttactt ctaatatagc ttccatatcc atagtatcca
    43681 tcattgagtg cccaactatc tttacacctt taagctcttc tcttatgtaa cttggtaatt
    43741 tatcagtttc atatgagtct acatttgctg ttgcaacagg cttataacct gctaataata
    43801 gttcttcact acttcctgat atatcaacta tttttttagg atttgatggt atcttaacct
    43861 ctcctttaac agattgtaca actcttgtct ctgatttatc tttatcctct gtattcttat
    43921 ctgaacatgc tgtaacaagt actaacgtag ttataataga tataaatatt gcaagtgatt
    43981 ttattttttt catgattact tctcctactt gtttattata cttacatgat attgattatc
    44041 ataagcacat atttattata gcatttttct atttttagtc aatattattt tgtaaaagga
    44101 taaaaattcc taattaatct taattatata tagcttagat aaggttttct tatattaaac
    44161 agtatttttt ttaagaattg gacacaaata caatgacttt tcagttaaac aatgataaaa
    44221 tgcaatttat agataatgaa tttagtaagg aggattcaaa acatggtaca tttagtatat
    44281 tgtgataatg caggaaaaaa aggagaaaag gtcttagata aaatcttggc tggaacaaaa
    44341 acaatggtta ttagaggtgc tgctggtaga aaaataccac atagtagagt atttgaagat
    44401 gagttacttt attttatgga aaaaggaact gcaaaaatat ctgcaaaagc cactgttaaa
    44461 aaggtagaaa actatgttaa attaacagaa gatgaaattt ctaaaatttt aaatgaaaat
    44521 caaccaaaat taaatttatc tgaaaagcaa aaagttcgtt ggcataaaaa atgtttatgc
    44581 ttagttgaat ttgaaaatgt tgaagaaata acacctcttg attttgacca tcaaggaaat
    44641 atggatgatt ggcttattgt tgaaaaaatt gaagatgtag ttgttggtac tagtatacct
    44701 tacaattatg agaagtctaa attttaaagt atcaataaca taatactata ttgctaaact
    44761 ctacttaaat aaaaaaagag tgcatttaat aattataaaa attattttta tacactcttt
    44821 tttaataata aatttaaatt acttactttc taatctttca tatacatcta taaattcttc
    44881 tgctaattgt tgaaaattca tagctgtcat taaatggtct tgtgcatgaa taagtataac
    44941 acttatttca gttttattac cattagcttc attttgtatc aattctgttt ggaatttatg
    45001 agctttatga attgattctt tcgacttttc aatacattct ctagctttat ctatattccc
    45061 gctctttgct tcttttattg cttcaaatgc tagccctttc gcatcacctg caaatccaat
    45121 tattccaaaa gatatctcta tcatcttctc atccatttat tcgacctcct aaatatctct
    45181 atagcttgat atgactaatc cattcttttc taaaaattct ttgacttttt tagatgtaag
    45241 tatcttgtgc tctttcgttc tatcaatagc ataagatgta gaatttaaaa tataatcatc
    45301 taaaaaagct gggtgagaca taatatccac tacatcatac ttcattattt cgtctatatt
    45361 ttttataaaa tattcttcac ttacattttc cttataaaaa ctatctatta aaggaactac
    45421 tttagaatac tctaaattat attcaaatcc acctcttatt ggaagattat acttggttac
    45481 aatcttctct attactgact ttaaacttac taaagtatgg atatgatggt gactatctaa
    45541 atgagatata tctattccaa gcccttttac cctatctatt tgagcacaaa gttctctata
    45601 aatctcatca caatccattt tttcaataat ttcgtctgta atcctcctaa taaaaaaacc
    45661 gtctttatca acaatagttt ttaagttaga taataatggt ctaccacaac ttaaagtcat
    45721 atgaactcca caatttaaag ttttattttc tttaagtaag cctactccat gttctacccc
    45781 aggcatattt gccatcatag aagtagacct aactatacca ttattatgag ctgaaattat
    45841 tccatagttc acagcttcac aatatccaaa atcatctgca tttattataa ttttagtcat
    45901 accataacct actttctata gacaactatc tattaaatct aggaagataa tctttatgag
    45961 cttccaacat ttcgtctaat atagtttttg ctaagtcatc agatggaatc aatgggttta
    46021 tacaaagtgc caaaagagca gattcataat cgccatatac agctgcttta gctgccaata
    46081 cttcaaatga tttgatttga cttactaatc catgcacaga atctggtaaa tatcctatac
    46141 ttattggttt tgggccattt tttgttatta cactacttac ttctaccgct tgttcatctt
    46201 taaaatctct tatagaaccc ttatttaatg tattaactac ttggatatct tttttgtcgt
    46261 tgtatataga acttattaaa ttacaagcag catcactata gtaagcccct cctctttttt
    46321 caagttgagg tggttttaca tctaattttt catccttata taattcaaat aattgttctt
    46381 ccaattcttt aactacttga cctctagctt ttccctcttt aaactctctc aactcatcct
    46441 caagcatttc tttagtttta tagtagtatc tatggtatgg acaaggtatt gcacccaaag
    46501 attttacaaa ttcagcatta aaatcaattg cctttatatt ttgcatactt atatctgctt
    46561 tgacaaattt atcaattgct tcttttgtaa catcttctcc atctaaagat acatttagac
    46621 cataaaccat atgattaaga cctgcaaaat ccatacttat tctgtcactt tctacattaa
    46681 atagtttggc aacatcattt tttaaatgaa ttggcacgtt gcatagacct atatatcttc
    46741 taaaattagt atatttgaat acagcctctg ttattattcc tgttggattt gtaaaattca
    46801 ccatccatgc atttggacat aattcttcca catcttttat tatatcaaat ataactggta
    46861 ttgtgcgaag ggctttaaat aaacctccag caccattagt ttcctgcccc ataactccat
    46921 gagataaagg aatagtttca tcttttattc tcgcatctaa aagacctact ctaagttgtg
    46981 ttgtcacaaa gtctgcatct cttagagctt ctcttctatc taaagtaaga tttattttca
    47041 tatctactcc agcttttttt accatcctct tagctagatt tccaactatc tctaattttt
    47101 cttttccagc ttctatatct acaagccata attcctttac aggtaactct tcatatctat
    47161 ttataaaacc ctctacaagc tcaggagtat aacttgaacc tcctccaatt gtaactattt
    47221 ttagtccctt tttcatctca ttataccatc cttacctata atttaatata ctctacaaaa
    47281 ccctatacat ttatttaccg aaataaaact acctagttgt taattttcaa tttattagct
    47341 tccatttttt cagacgctat gacaaatgga atatatatta atattgataa tattaaatta
    47401 aatagtgcta aagctccgcc agtccagtca cctgtagcca ttgctccacc taatatagga
    47461 ggtgttaccc aaggtatttt aggtaaaatt actggtgcta ctaatccaaa atctattgct
    47521 aagtatgata cagcactaca tattactggt gcaagtacaa atggaatcat aaatatagga
    47581 tttaaaacta ttggaagtcc aaatatcaat ggttctccta tgttaaatat tgcaggtgga
    47641 ccacctaata ctatcatctc tttacgtctt cttccagcta ttaataaagc tactattaat
    47701 cccaatacca ttccagaacc acctaaatat acaaatgcat caaagaatgc acctgcaagt
    47761 acatggtatc cttccgttgc tcctgcttga gccaaggcag cattttctcc acctaatttc
    47821 ataagtatag tttctgtaaa aggaagtgct atatttgcac catgaagtcc aacagtccaa
    47881 agtatatgaa ttataaatgc aataagcatt gtagaaccta gactatcagc tacatttgat
    47941 agcggtgctc ctaaataagt atttaaaata tctgttaaga aacttccatt agaaagcatt
    48001 tttataaata ttcctattac agtaaatatc attatagtta acattcctgg tattaacttt
    48061 gcaaatgatc ttgaaactgc tggtggaact ccatctggca tttttatagt taatttcttt
    48121 accttagata atcttacaaa tatctcagtt gataataatc ctattaatat agaagagaat
    48181 aaagctgctg tacctaagta ggtttgttgt atcattcccc atccttcaac tactgtagtt
    48241 cctccctcag gaactatttt accaatttgt ggagccatta taaaaaagat agataaagct
    48301 ataagcccag attgtaaccc atcactctca taagattttg ctagaaaata agcaacacct
    48361 attactaaaa atactgccat tgtacctatt gctccccacc aaaggtcgcc tccaaatgtg
    48421 gtccagtttt ctccaaatat acttgccatc aaatttttgt atgcttccaa tggtaagtta
    48481 tttatcaatg ttcccaatgc acctaccata gttataggta tcattactat aaatgcatct
    48541 cttactgcta ctaagtgcct ttgagctcct attttagcag ctacaggcac tatatattta
    48601 tccataaatg acatgaattt ttccatttta catcctccag tacaacttta ttttgaacta
    48661 tttaactcta cagctctatc taacacagct tttccattac atcttccata atccatcata
    48721 tttataactt ctactggaat attatatggt tttgccattt cagttgcttt tcctaataca
    48781 tatctaactt gaggccctaa aagtaagaca tcacaatttt ctatttcatt ttttaagttc
    48841 acttctgata tagcccatat ttttgcttct ataccgtttt cctttgctgc atcctccatt
    48901 tttgtaacta ataaactagt tgacattcct gctgaacaag ctaacattat ttttatcatt
    48961 ttttcccctc caaattagat tcattaaatg ttttttaagt cataagtttt ttaaactata
    49021 catttaaaat atgcttttct attaattttt tcttaatgta tatatcttat ttttaaatgt
    49081 atatacattt aattcttgat attattatat accaaatgta tatacatttc aagaaaactt
    49141 tttcatttta tttaaatttt attcgacaac agcattcaaa gttaatgtat atccatagtg
    49201 atatgtgtgt gagtactcaa aaatctcact attttgtaac ttacaaattt ggcttatata
    49261 taaaagtggc tctggttctt ctaattttag attatccata cactcttcat tcgatattac
    49321 agcttgtact tctctctctg acatggctat cttgtatcca cacacttctt caacaaaatt
    49381 ataaagagaa ttttcacaat gagtcttatt taaatttgga aataacttaa ctggcatata
    49441 agtaatcata tatgaaactc ttgcatcatt ggctaatctt actcttttta tttcccaaac
    49501 attttcttct aaacttatat tcaacttact tgataaattt tcatctgcct gaactatttc
    49561 aagagatacc aatatattag atactttata gcctttactt tccatctctt tagtaaatcc
    49621 acttacagac gatatattat catatatatt atttgcaata actatactac ctacacctct
    49681 ttgtctagtt atatagcctt ctttaactag attattaagt gcagacctta cagtcattct
    49741 agttacatta aattcttcac tcagctgttt ttcagaaggg attaaatccc cttctttcaa
    49801 actgtctgaa tttattaact ctctaacatg attttctata actttataaa ttggctcttt
    49861 catttaattc actccttatt aaatctttta tataataaga taattttata tacaaacaaa
    49921 gaaaatgtct atacatttaa gataatttaa attttattca gaaatcttgt acaactttcc
    49981 gagttttaat aaagatataa atagacttat cattaatata ccttttaata tacttgataa
    50041 ggttatactc caccatacac catttagacc caacatttct ggtctagata taaagtaagc
    50101 caatggtact cttgcaccag tcagtatagt aattataata gaaggtatat aggttctccc
    50161 taatcctttg aaagctcctg ttgttattat ttctaaacac ataaaaaatt gagaatagcc
    50221 taatatttta agataaattg ctcctttttc tattgcctca ctttcatcta taaaaataga
    50281 aaatatactc tctcctgcaa atactaatac taaagtagtt attataccaa gtacagaaga
    50341 tacagttata gctattttgc aacctttatt tattctagaa tacttcttgg caccataatt
    50401 ttgtccaaca aaactgccta atgcaactga tagcccttca gccgtcatcc aggaaattgc
    50461 ttctatttgt gagcctactt tttgtactgc aacagccaca ggcccccatg atgctactat
    50521 tactcctaat agcattgaga atactgtaaa cataccactt tgtattgcta taggtaatcc
    50581 caatttatac agaactttat aatatttagt ctctatttct ttaaatagtc ttaatttaaa
    50641 atattcttct ttacttttaa ctattatata tataaaacat gtagtaacta ctatctgagc
    50701 aaatacagtt gcaatagcag ctcctgcaac accaagtttt ggagcaaatc cccatccaaa
    50761 aattaatata gggtcaagta taatattagt tattaatcct actgtattaa tacgaaaagg
    50821 agttttacta tttccaagac cattgaaaat tgcagtaaat actggattaa taaaataaaa
    50881 taccatacct agagctacta ttatcaaata ctgtctagac atggttataa cttctaagtc
    50941 tcctaatcta aaaaaaccta tcaattgttt attaagtact attaaagaaa ttgtatatat
    51001 tattgctagt ataatattta tttctattgc tgacttcata tatgatttag tagtgcttat
    51061 attatttgca ccaatacttt gtgctacttt tatctctcca cctacttttg aaatcataat
    51121 aaatgccatt gcaagccatg gaaaaaaacc agctgttcca actgcagcta ctgctttact
    51181 tccggcttta ccaacccata tcatatctat catgttatac cccatctgta taaatgaagt
    51241 ccccattatt ggaagtgata gcttaagtaa tttttctgta attcttcctt ctgttaaatt
    51301 aagtctgtct ttcattttcc acctctttta tattaaattt atttgcttat atagctagat
    51361 taatcatttt atttgattga gttcactatc cttcaattta tattatcata tctcaggatt
    51421 gcatctcaat catacttatg tttaagatac agtattttac ctatctatga taaaattaat
    51481 acttatgaat ttaaatctag ccatttcatc aatacatatt ctaaccctta acttaacttc
    51541 aattaagtat acatatcgta accaattaga atattttata cggtatcttc cattaataaa
    51601 caaccaatat caatgattta tatataattt tatttataca taaaatagta tactataata
    51661 ataaaaaaac tatatttgaa tgctttaagc atgtttacta attgtaattt ccacacaaat
    51721 ttgataatat atgaatatat tatggtgaaa tttatagata tatattaatt atggggagat
    51781 tgtgggaggt tcttttcaca agacatttaa gctatgaaaa taaataaaaa attcaataga
    51841 gttgatatgc aggtttcaat actcactgca attattgtaa taatatcttc ctcatgtgta
    51901 tattttttta attatacact aacttataag agcatgatat actctttaag tgagaggtca
    51961 aaacatatct atgaatatgt agaaagaaat attgataaaa atacctttac tgaaattaat
    52021 tctaaagaag accaatcaaa aaagtcttat aaaagttcta aaatcctttt agaatctgta
    52081 aaaaatacta ctggagtcat gtatctatat actgctaaaa aaacaaaaga tggatcacta
    52141 gtatatgttg ttgatggttt aaattcatct aggagtgatt ttagaagtgc tggtgatttg
    52201 atagaaaaag agatttgggg agaccttaac aaagcattag ataatcatat attgttacct
    52261 aaaaagatta aatcaacatc ttggggatac atatttatat cgtattttcc tattcacaat
    52321 gatgttggta aggttgttgg agtaatagga attgaatttg aagcaaaaca tcaatacaat
    52381 acatttaaat ttataagtat agttacacca ataatagcat tattaacttg tctaatatca
    52441 gcattgattg cagtgattct atttaaaaga atatctaatc ctacatatac tgattttgct
    52501 aatacagatt atttaacagg cctaaaaaat agaaatgctt ttgaaataga tatgaataac
    52561 ttaaacaatt caaatctaaa gaatctaata tctatcatat caatagattt agacggttta
    52621 aaaaagatta atgataaatt tggccatcag acaggagact tatatattaa gcaagcaagt
    52681 aaaattatac aatatgtaat aaataaaaaa tatatagttt atcgtattgg aggtgatgaa
    52741 tacataatta ttctaaaaaa tttatctcac aaagaaataa ataatattat aaataatatt
    52801 aatttaaaaa tagttgaaga aaactcaact aatgaattaa aattatcaat gtctattgga
    52861 tatgctattt ttgatgaaaa agtagatgtt tctttatttg atacatatcg tcgtgcagat
    52921 tctcaaatgt atgataacaa aaggaaaatt aataaacaat atcaataata aatttaaaaa
    52981 gtctattctt taaataataa atcacaagaa tagacttttt taaatttgtg tttttataag
    53041 ataaattatt taattaaagt ttgttctttt ttctaaacga ttctttataa attcatatat
    53101 tactttcact aatatatttg tgactaatat taacacagct agtgctgctg cctgtgcagt
    53161 actcccctta tcatctagtt gtacaattga tattgcagct acttttgaac ttgcagtata
    53221 tataaaaact aatgctgata ttgtcaccat ggagtttagg aaaaagtaca taaagttctc
    53281 taaaactgct ggcaaacaaa gtggtatagt tacgttctta agaattgatg cactgtttat
    53341 tcccattgat ttagctacca tatccaattc tttatctact ttcttaatag cagtagtagc
    53401 tgttataaat gctactgaaa agaaatgaac tatattacat ataaccatta taagtaatgt
    53461 tccatataat ccattaaaca aattcttaat gtatattgta tccataaaat taaactctaa
    53521 tttattaaaa aataatacat aagctatacc taaaactaat cctggcaatg ccataggcaa
    53581 tgtacttaaa aaatacccaa atgattttaa atatggagct ttttctgttt tttcattcaa
    53641 atatgcaaat aagaaaacaa agctcgtccc tagaatagct gtcaatagtg acatttttat
    53701 tgaatttata aatggtttaa tcccatcaat actgtttttc atagaaaaat gttccagtgt
    53761 cagtcttaaa tcatatggcc aaactgatat taatgaagat aagattacag ttaacattac
    53821 agctattata ccaaatgcaa taattcctac aacccctcct aaaatactat ctcttaactt
    53881 attagtttta accttatatg gaattgactt tgaagttata tatgaatttt gatttttttc
    53941 aattttttta gttactataa acgatattat tgcaggtatt aaaagtaata ttgatacagt
    54001 tgctcccatt ggcatattct gttgccctat aatttgctta tatacatcta ttgataacac
    54061 attaaactct cctcctatca cctttggagc tccaaaatct gtaaaacata atgtaaacga
    54121 tacgaatgca gaacttatta aagagtattt tatacttggg attgttatgc ttataaattc
    54181 tcttatcttg cttatcccca tagtattact agcttcatat agtctataat cggttgaact
    54241 tagagatata taaagtattt gaaatgattg aggaaataca tatataattt gtgccaatat
    54301 tattcccact gctccatata ttggaacatg cactccaagt aaattagtta ccaaaccttg
    54361 atttccaaat aaatatacta gacctattgc atgcatcatt gttggagcaa acataggaaa
    54421 catagctaat gatttaaaaa aacctttaaa tttaatcttt gttctagtta atgcatatgc
    54481 atatactaat gcaagtgtta gtgatattac agttgatata actgatacaa atactgtatt
    54541 ttttagagaa tttaatagag attcagaagc caagtattct tgaaagtttt gtaatcctac
    54601 aaagactcca tctttatcta aaaaagcctg tacaaataga tatattatag gtgctaataa
    54661 aaatattact aaagacataa ctaatactgt aagcatacat ttttttatat ctaaattcat
    54721 ttttgattta agacttatac ttttagaact tatagcatta ttattcatac gcttatgctc
    54781 ctttaacttt tggatacttt aaatagtatt tttcgtctat acttattttt acaaggtcac
    54841 cttctactaa atttaaatta acccactctt ttatagaaac atcacttatt atgaaatttt
    54901 catttaattt atcttttatt tcaactctca ataaattccc tctaaactct atatttgata
    54961 ttatcccttg gtagttttct tttgtcgatt tctctatttg aacatattct ggtcttacag
    55021 ttaatataga atctccatta tcaaaacagt ttgtatctcc tataaattga gctgtaaata
    55081 ggttttgagg attttggtat atttcttctg gagtccctat ttgcattata tctcctccat
    55141 ttagaatagc tattttatct gccatagtta tagcttcttc ttggtcatgt gttaccatta
    55201 ttgtcgttat attaagttct ttttgtaatc ttttaatgtc cattctaagc ttgtgtctaa
    55261 cctttgcatc taatgcggac attggttcat ctaaaagtaa aaactttggt tcaagagcta
    55321 atgctcttgc tattgcaatc ctctgttgtt gtccacctga taatgcttta gggtatttat
    55381 gagcttcatt agtcaatcca actgtttcaa gtacttcctt aactttatta tctatttttt
    55441 cttttgaaac ttttctctct tttagtggaa aagctatatt gttataagca gtcatattcg
    55501 gaaataatgc atatgattga aatactatac caaaacctct ctttgatggc tctaaatttg
    55561 taatatcttt atcctggaga attatagttc cactatttac atcttcaaga cctgctatta
    55621 ttctaagtaa tgtagtttta ccacatccac taggtccgag taaacataga aattcccctt
    55681 cttcaatatc caaactaata ttatttaaaa cccttttttg gtcataactt ttaaacacat
    55741 tatttatttt taagtaactc atatcttatc tctccttata tagttatttt tttattttaa
    55801 attttatatc ctactttttc ttctaaaatt ctttcatcta aattattaat ttaaacctta
    55861 cttactttca gattttgaac cgtattttga ttcccaagtt ttaagtactg tacttctgtt
    55921 tttggcagca gagtttaaat caacttcttt tataagctgt tcaaatggtt gtttagaata
    55981 accttttgga actggattat ctgtctttat ggaagtaact gctacttcat gtgcatatct
    56041 atccatagct gaatcactta ttgcccaatc taaaaatagc tttgcttctt tttttatatt
    56101 atctttttta actaaggcat ttgcttctaa gtcccaacct gaaccttcct ttggaaatac
    56161 aacctctata ggttctccac tagataattg ttgtgtagct ctatatccaa gtgtaatacc
    56221 tattggatac tctccagagc cagctaactt cgcaggtttt gaacccgagt gtgtatatgt
    56281 ggctatattt ttatgtaatt tatccatgta attgtaagca tctttttcac ccatcatttg
    56341 aataagccct gatactgtaa gataccccgt tccagatgat gctggatttg gcattgttat
    56401 aagtccttta tattctggtt ttattaaatc ttcataactt tttggaatat ctatacctag
    56461 tttcttaagt tccttataat taactactat tgctgtctct gggacactca ttcctaccca
    56521 agatacatca tcttcagtat ctttaaattt tttatcaact ttatcataat ttttaggctt
    56581 atatgcttca agtgcaccat ctttttttag ctctatcatg ttcattgctg ataatcccca
    56641 tataatatct gcttgtgggt tatctttttc tgctaatagt tttgaagcta ttacaccatg
    56701 agaatccata actatctcta catctatatt gggatactct tctctaaaac cttccatata
    56761 ataagctatc tgctcttcct ctagtgctgc atatattgta agtttattat ttccttttcc
    56821 ttgagtagtt tgtggtgttg ttgatatgca acttgtcata aatgtaatca ctacaatgca
    56881 cagacaagta aatataaatt ctttcatatc ttcccccctt tgtgtttttt ccttttttta
    56941 tttgtttttt attgtttttg cttatgttta tataatacac ctaatttgtt aatataaagt
    57001 taagtgtaaa ttaacagttc gttaattttt agaaattttt gttaaatata ttatttttag
    57061 ttaaatacag tctgttttta aacaatatat taatttttat agtatctaca ttttaattta
    57121 aacataaaaa ataagtctaa cctctataaa gtagaatgtt agacttattt ttataagtat
    57181 ttaattatac aaccaaatca ttttttttct tttcttcaag tgctttttta agcaccatat
    57241 ccttaacctt cataagtcta gtagtacttg ctctctcatt ttcttcaagc ttcatagcta
    57301 tatacttaat agtctcaatg taatttggta tcattacatt ttcaagagca ttaacacgtc
    57361 tacgtgtctt ttctatctct tgtgcaagaa gctgtgcact tttttcactt tctgcaagtc
    57421 ttaaaagtgg ttgcatagca tctgagaaag cttccatagc agaatctaat tcacctgatg
    57481 taaaagcaag accataagga taaatatcac tttgattgtt ttcagtttta aagtcaaaca
    57541 caggaacatc aacactcatt atatttctcg tagaaacatc tactgaaact gattgctttg
    57601 gcatcataag tgctgaaccc agatactcct gactcataac tgctcttgcg attataaagt
    57661 ttttataagc agtatcaaga gcattttctg cttcttcacg caatcgctta ttttctctta
    57721 caatctcaag aaattgtttc ataagttcat caagtttatc tttaagaagc ttatgacctc
    57781 ttgtagcagt tttaaggagt ttcttaagtc tagtcatttc catacgtgtt ggatttatat
    57841 ttaatctagc cacagatatc actccttagg catatatttt tcaaaatatt catcacggat
    57901 acgtttaagt tcactttttg gtaacatact aagaagcttc catccgattt ctaatgtctg
    57961 ctcaatagtt ctatcagttc taaatccttg tgaaacatat tctttctcaa attcatctgc
    58021 aaacttagca tacattaaat caatctcaga aagtgcagat tcaccaagta tagccataag
    58081 ttcttttgca tccttacctc ttgaataagc tgcaaaaagc tggttcattg tatctgcatg
    58141 gtcttcacga gtcttacctt taccaatacc tttatctttt agacgagaaa gtgatggtaa
    58201 aacatctata ggaggctgta tatctttttt ataaagttca cggctaagta taatttgccc
    58261 ttctgtaatg tatcctgtaa ggtcaggaat tggatgtgtc ttatcatcct caggcattgt
    58321 aagtattgga atcatagtta tagacccttc atgccctttc atttttcctg ctctttcata
    58381 catagtagca aggtcagtat aaagatatcc tggatatcca cgacgtcctg gaacttcttt
    58441 tttagctgct gaaacttcac gcaatgcctc tgcatagttt gtaatatctg ttattataac
    58501 aagtacatgc atatcttgct cgaaagcaag gtattctgct gctgtaagtg ccattctagg
    58561 tgtagataca cgctctacag ctgggtcatt tgcaaggttt acaaacacta cagaacggtc
    58621 gattgcacca gttgctttaa aatcgcttat aaaaaattca gcatcttcaa aagttatacc
    58681 aactgcagca aatacaacgg cgaacttaga atcagttcca cgtacttttg cctgtcttgc
    58741 aatctgaact gcaagattgg catgtggcaa accagaccct gagaatatag gaagtttttg
    58801 ccctctaacc aatgtattaa gtccatctat agcagagact ccagtttgta taaactcggc
    58861 cggataatca cgtgctgctg gatttattgg agctccattt atatcaagtc ttttatctgg
    58921 tataatttct ggacctccat caataggtct acccattcca ctaaaaacac gacctaatat
    58981 atctggtgaa actccaaagt caagactctt tcctaaaaaa cgaactttac tttcagcaag
    59041 gtttatacct gttgagcttt cgaaaagctg aacaagtgca gtatctccat ttacctctag
    59101 aactttgcaa cggcgaattt ctccatttga aagttcaatt tctccaagtt catcaaattt
    59161 tacaccttct acgccgttta ttaacataag aggaccggct acctcttgta ttgacttata
    59221 ttcctttatc atagttcttc agcccccctc tttttaataa ggtctgcaag ctcgttgtct
    59281 atttcatctt caattgaatc gtaagctgca tctatatcgc cttcttctac atacttagca
    59341 cgaccaatct tttcaagaac tgaaagcttg ataatatcgt ttatagtaac attttgcttg
    59401 atagcatctt gtgctgactt atagaatttt aatataagtc ccatcattct atattgttta
    59461 tgaagtgatg tataagtatc aacttcatgg aaagagtttt gatgaaggaa gtcttctctt
    59521 atagaacgag taacctctag tatcaatcta tctccatctg aaagtgaatc aacaccaacc
    59581 aattgaacta tttcttgaag ttcagattca acttgtaaaa gtttcatagc ttgagctctt
    59641 tgagctgaga aattatcatt tacattttta tctgcccata catcaacttt ttcctgatag
    59701 agtgaataac tagtcaacca gttaattgct gggaaatgac gtttatatgc taaagatgca
    59761 tcaagacccc agaatacttt tactatacga agcgttgctt ggctgacagg ttctgatgta
    59821 tcaccaccag gaggagaaac tgcaccaatt gctgtaagtg ttccttctct ttcatccata
    59881 cctagacaat ttacctttcc tgctctctca tagaattgag caagacgtga acctaagtat
    59941 gcaggataac cttcttcacc aggcatctcc tctaaacgac cactcatctc tctaagagcc
    60001 tcagcccatc ttgatgtaga gtctgccata agtgcaacac tatatcccat atctctaaaa
    60061 tattcagcta ttgtaatacc agtgtatata gaagcttcac gtgcagcaac cggcatatct
    60121 gatgtatttg ctataagcac agttctttgc ataagtgact cgcctgttct tgggtctttt
    60181 aattcaggaa attcaagaag aacatctgtc atttcatttc cacgctcgcc acagcctata
    60241 tatacaacta tatctgcatc tgcccattta gcaagctgat gctgtgtaac agtttttcca
    60301 cttccaaaag gtcccggtat agctgctacc ccaccttttg tgattgggaa aaatgtatct
    60361 ataactcttt gacctgtaac aagaagtgaa tctggtgttt tcttttctct atatggtctt
    60421 tcttttctaa ctggccattt ttgcatcata gttacaggta tatcattacc tttttcatct
    60481 gtaataacac aaactgtatc ttcaacagta aattcaccag aatatatttc ttttatagta
    60541 cccttaactc cataaggaac cataatttta cattctacta ctgctgtttc ttgaacgcca
    60601 ccaattatat ctcctgctac aaccttatca ccaacagatt ttttaggttt aaattcccac
    60661 ttagttgttc tatcaagtga agatacttca acacctttag ttatattagt acctgaaata
    60721 tcatacattt tttcaagtgg acgttgaata ccatcataaa tagttgaaat aagacctggt
    60781 ccaagttcaa cactcatagg cattccaaga gaaactactt cttcccctgg tcctagaccg
    60841 gaagtttcct cataaacctg tatagaagct ttatctccat gtatttctat aatctcgcct
    60901 ataagatgtt tatctgaaac tcttacgacg tcaaacatat tcgcatctct catgccctcc
    60961 gctacaacaa gcggtcctga cacctttaca attgtgcctg ttttcatatt ctctcacctg
    61021 caatcatata ctttatcaaa atttaaatat tccctcctat attttattag tacatatatt
    61081 tagcaccaat aaaatttgag attgtctttt attcaacttt catctattct ttaaataata
    61141 tatctgcacc tatagcacgt ttactagatt ctcttacttc attcatacct aatcccatac
    61201 ttcctccaat acctggaata actataatgg caggaagttg gtaatctttg tattttgcta
    61261 ttgattcttt tgcaagcaat gatgcctgtt ctgtaatata aattatcgca tattcatctt
    61321 caaccaactt atgtatactt tttttaattt cgtcactatc ataggctggg aagatatcta
    61381 ttccaagagc taaaaatccc atgatgctat ctttatctcc aactactcct accttataca
    61441 taagcatccc ttagcctttc ttttatagta tctgcgtcta tattattaag cttactagtc
    61501 aaaataattc taattgtttt tatctctgat tctttagcat ataaatatgc aattagtggt
    61561 tctaatgtaa gaggcttaaa ttttgctcct ctaacatatt ccataagata gtcatcacaa
    61621 agtttttcaa agacagtaaa accactatcc atgccatttt tacagacatc agagtaagaa
    61681 gtagatttaa aacatgatgc tggagtatct gaggaaaaag cttctaagaa tttttcctta
    61741 tcaagtgagc cacctgaaac aaacacattc ataaacatat caaaatcttt cttcatattt
    61801 ttgactctta taaaactctt tagatttgtt aaatcacaaa ctttcataac ataatctatt
    61861 acaaatttgt tttggctttc tttagcagtt tctttcatac tggtaaaggt tgctttatct
    61921 agaacaatat caaccatctg gccattttgt gtcttattat aaacatcaaa tgcctctaat
    61981 atagctgatg acattatctt aggtaaatca ctaaaactac gatttgctaa agattctcta
    62041 agcttaataa gaggaattgt acctccatca attaaatatt tctcaccatc tacaccagaa
    62101 atttcttctt taatcagcac ttttaaattg tgatagtcat ttttataaag aaatacatca
    62161 acaaattttt cttcaggagc tagactttta agaatttcat atgtctttga taattcttga
    62221 gtcaaaatat tctcaaaatt atgtatatta ttatttgatg tatcagcata acctgcctca
    62281 gctataattc ttaaagagtc ttcagggctt ttggcttctg ccatttgaat aaacatttgt
    62341 ctgttaagaa gctgtttttc aagcactctt attcgtgcaa cggcataggg ataagttttt
    62401 tcttctgcca ttttttaacc cctccttaat taaacaaaat ttctgctgca atttgttcaa
    62461 tatattcatg ctctactgca attatggctt caaatgaata attgtactca atgtcacctt
    62521 ttttaactat gaaacctcca gtaatatctc tagtttcatc agacacttta atgccttttt
    62581 ttgatagcac atcttttaat gtttttttat cttttttaga taatactatc tctgaatcat
    62641 ctgtacaatt tgatttttca atcatgttta gtataatctc ttcatattca ttatctttaa
    62701 gatttaaaag tttgttttta gcttctaaaa ttacctcttc taaaatctct tgtttttttg
    62761 ataatatttg ctttttagcc tccatataag ctcctgatat ttccttagaa gccgcttttt
    62821 ctgcttctgc ctctgcaagc tttgtatagg aagccatttc cttttcagct ttttcattag
    62881 ctgaatttac ttttaaatct gcttcacttt tagctttatc caatatttct tgtgcttctt
    62941 gtttggcatc agctataatt ctgtcaatca ttttttgttc attacccata ggagtgcttc
    63001 ctccttttca ttttttttac ttatgaatta taatttataa ttaaccaatt gcaattccat
    63061 tgtaagctaa gaaagaaaca agtagtccta aaagagcata tgtttcaact attgctgcaa
    63121 atgttatagc tttaccaagt tcttcaggtc tttttgcaac tattccaact cctgctgctg
    63181 ctgctttacc ttgtgctata cctgatacta aaccaactaa accaattgga agtcctgcca
    63241 taagaagttg taaaccttga gctgatgttg gtattgcatc tccaccaata atccctgctt
    63301 ttgataaaat aaggaaagct atgataaatc catacagacc ttgtgtacca ggaagaagtt
    63361 gtaaaactag aagttgaccg aatttactag ggtcttctgt tacaacacct gctgcggctt
    63421 gacctgctat tgaagtacct ttagcagaac ccatacctgc aaataatgct gctatagcag
    63481 ctcctgatat tgccaaaaat tgaccatttc ctaacgcgtt taaaaattcc atatttacat
    63541 ctccttttta ttaatgcttg tatattttgt tttatactta aacggaacaa aaggaactcc
    63601 tccgccttca taaaacttat tgaaaaattc tacatactgt aacctacttg tatgaacata
    63661 tgcacctagt gcatttataa gaaggtttat tgtgtgtcct acaacaccaa ttacaactgc
    63721 aagtatcgga ccagctatag ctcctaaaag gttgataact tgtgctataa ctcctgttgt
    63781 taaacaaagc gccataattc ttgtataaga taatatatca gcaaaataac ttgttatacc
    63841 atagagactt gaaaatcctt taaaaagctt aacaggaata cctttaaagc ttcttccttg
    63901 agtaagtaca agacctatag cacctataat tgctaaatat tttcctactt ctgaaaatac
    63961 acctatgtcc cctgcaacaa aaggtattat aagtagacat acacctgtta tacacaaata
    64021 ccaaaaacct atgtcaaaga ttgcatctat tacctttcca cttcttatta atgtatatgc
    64081 ttttattccc aaacctacat atatatggat aataccgaac agtaacgaca ttcccataag
    64141 tgtcataaca tcctctaaag gattaattaa tgcttttata ggaattaaat cccccaaaat
    64201 acccccgaat ataagacccc atacagtagt tgaaactcca cagataccaa taagtttaat
    64261 taaatttcct tcaccttttt gtatctttaa tttatataca acaaatccac aaactactgc
    64321 aattattaac ccatatgcgg cgtcacttag catcattcca aagaatacaa caaagaagaa
    64381 agttaaaatt gtatttgggt caatatcttt tgtacttgga tagctgtaca tgttagttac
    64441 actttcaaaa ggtgttacta atttattgtt ttcaagcaga attgggaagc cttcttcttt
    64501 acttccttct tcagtctgaa cacaacaatc aaacttttct gtaatttctt taattaattc
    64561 atctgccctt ttgcttggta accaaccatt aagacagaat gttgtttttg ttttgacaag
    64621 tctctctata atcttttttt ggtctctttc tatagtgtaa aaatcatata ggttttcaag
    64681 catgtgtata ctatctgcat gtcttttaat ttcctctgtt ttgtccttag aaacgttttc
    64741 ctgtctaatt atcatttcat tgtatttctt gatagcttgt aacggtgtac cttcttcatt
    64801 tggtaatgaa accactgaaa aattaaattc ttttaaaact tctaaaacat catcaaaagt
    64861 ttcttttaaa gtaattaaat aaacatagtt cattaacttg tcagaattga caatcccaat
    64921 aacactttct ggtatttcat cttctaattt tactcttaaa gcatcaatgt ttgcgcttat
    64981 gggcacagtt ccaagtatag tttttgtata cttagtttcc atattttcaa taggaatatc
    65041 aaagttcacc catggttcta acatactttt attgttgtta agtaagttta tgttagacct
    65101 tattgtacct atctccttgt gcaaattgtt taactccaga gcttcagcat aaacttgccc
    65161 ttctttttca ctatcaattt tgttaaactc tctttttggt gcaagtaggg attttttttg
    65221 tttcactagt gaagaaacaa tattaattgc ttggtttaca tgagacattt tctgctctag
    65281 cattataact tcagattctt cactatcttt tacaagataa ttttttaatt cctcctcctc
    65341 agtaagaaag gcactatcat caatctgaac aaacccacgg ttcataagta gctttaaaat
    65401 ctggcttttt tgagcttcta agccaacaat agatattttg ttcatattaa ctattgccat
    65461 attatctcac tacctttcca ataaccgctt caatagcctt actttttctt tcttcagcag
    65521 ttttttcaat tttcagacat tcacttttag cattagctaa gattttatca acctctgctc
    65581 tactgtcggt gactgccttc tcaataagag tttcttccat aagctttgct tgttttttta
    65641 catcagcaaa tatgctttca catttgttta cagcatcagc tctgatagac tctgcatcag
    65701 taacagcttt tactttgata ctgtctgctg tctgctctgc ctcaacaatg ctttttatta
    65761 tttcaaaagc catccaaatc acctctctta cctttttact atgtatataa ccaattagat
    65821 ttttttattc taattagatt actacctcat tgttaaagta taaacagcat ttttatataa
    65881 taattaatat ttttcaacgt ttatttctta ataaaacata ttttagaata gtacttgcct
    65941 agatttacat ccttttttga cttactttta gactatgaca tgagtatggt ttttaaatac
    66001 cattattttc aataattata atttaaagcg atatagtttg tcaatagcgt tattttgttt
    66061 taaaattaac gttatgtcga cattttaaat ttttatttta ttttttttat aattttaatt
    66121 taatttatta catataaaag ttttttatat tctttttcaa cctttaacca ttgaagttta
    66181 ttgttttttt tgaattttta gaaatatctc gcactatttt atctaacttt tataaatcat
    66241 caagctatat cgatttttgt caccttattt ttaatctaat ttttttatat ctagttagaa
    66301 ataaatcaat atttttaaag attgataaaa cttacaccta gttctaatag ttttatttta
    66361 aggcaatgta ttaatcttaa gtagataata tcattagtta ctctatatat tcgacaacat
    66421 tataaaaaaa tcctcgtacc aatcactcca atcttcaata ggatatgctg ctcctccatt
    66481 tacacttttc agtagattgg atgtatattc ttctcccata gaaaaaagtt cccctactgt
    66541 catatcataa cctctagatg taacaacctt acaaaatgaa tccataacat cacgttgttc
    66601 tcttgttttt ataagttcct ttctaaattc atcatcattc ttagcttctt ctaaaatttt
    66661 agttaatttt tcattcatta tgtatttctc cttttaagta gtctttctgt aaacttcaat
    66721 aagctttata taaaaaggaa gccatcctaa ataaactaag atgacttcgc ttaactgtta
    66781 gcaacacatt tactagtata aaataattgg tgtacctggt tctatatttt catatatttt
    66841 ctttgcctta gaaaaaggtg tatttacaca tccatgtgaa cctgaagact tatatatact
    66901 tccaccaaaa gaatttctcc aagtagcatc atgaatgcct atgttcccat taaatggaag
    66961 ccaataactt acaggtgaac tgtaaccttg accagttaat gaaacatttc tagccttata
    67021 attaagagga tatatacctg atggtgtagc aaatcctcta gctaaatcac ctgttacaac
    67081 gtctgttgat acaagcattt ctccattttt aaagaaccac atatgttggc cagaaagact
    67141 aatctctaca tatgtatttc caatatcatc tttattacga gatacagctt tttgtatgta
    67201 aataggttct cttgtaacat ttttaccagc ttttacaaca tcaactaatg cttcagcttc
    67261 tttcttttta tctatcaacc aaccataaat tccacctgaa attgtaatat ctttacctgt
    67321 ggcagtttta aatggtcttg agtcaccaaa agtactgtat tttttactta aacctcttgt
    67381 atattctacc atagcatcat tatcaaaagt tatgtcataa ttcttttctg agtctacttt
    67441 taaccattta ttaatttcag aaccatctaa agtttcctta cggtcactaa aatcatatgt
    67501 aacgactaca ccaacatact tatctaatgt cttaatagct tgtttcaact tttctgaatc
    67561 ctttgtgttg tttggtgctt cataaacttt actttcatcc agagaaatag aagtattccc
    67621 agaattaatt gagttttgta ctaacttata aaaatcatct tttttcactt tgtttccaag
    67681 tacctctggt actataacat aagcgttttt agtgctatca tacttaggat aagcactctt
    67741 aggttctaca acatttttac tatcaaaaca agataaagca ttaaaccttt tttctagtaa
    67801 atctttatta tatgaaagag ttatcatatt attatactct ttagatttaa atattccacc
    67861 aatccatcca atagcgctct gactatcttt tatcttttga actttatcat ctggtgtaaa
    67921 ttttaaatca atatcttttg cttttatttg ttctttctta ttgtttcttt cctctaagtt
    67981 taaagtatag gaattagtac tagatgctaa caaatcattt acttgctcaa ctgtttttcc
    68041 tgaagccttt acgccattaa tagtcgttcc aaagtaaaaa tgatttctaa aaaagattgc
    68101 cattcctata taagctacaa acaaaacacc tactactata gctatcttaa attttagatt
    68161 tttttgtcca ctttcttgtt ttacctcttt tccatcaatc ataattaaat ccttccttac
    68221 attgtaatta cctatgtacc ttattggttt ttaaacaaaa ccatgtatgc cttattttat
    68281 atacccaaat tactgtatca ttatttatat ttttaaatat atagttattt ctataaaacc
    68341 attttatctt ttagataaaa tgattattat aaatcccccc tttcaaaatc ccttaaacac
    68401 aatctctgct atattaatat attaccatac ttttaataat cgtgggacta tctatctatt
    68461 tttgtaacac taaactacta ttttaaaaaa gaaactaatt ttgtgttagt ttctttatct
    68521 taacttattt ttatattgaa ttttagctta acataacgtt gttatataga ttctatttaa
    68581 ttattttagt ttctatatct tttccatctg catattcttt aagtaattcc ttagcatatc
    68641 caaaatattc ttcaatatca gattcttgac taaaagaaaa tatacaaatt aatatttcat
    68701 ttgcatcttc tgttatcatt gctctttcag aatcactagt tggactccca aatttaccca
    68761 ctgaatcaga caatatagga agattttcta tatttattaa ttctttccca atgcccttat
    68821 actgttctcc ttcatctcct acagtaaaac atactggtga atgtaatttg cttacattat
    68881 atgtacctac tggatataat gattttattg atattaaatt gtttatttct actatattat
    68941 ttattgtata aagacccttc ccttgaataa ttcttctaac caatgcttcc gaagatgttc
    69001 tatatctact tggatttttt ccaagctctt tataagcatt tcttgcatct ttaattttat
    69061 ctagtttcgt aatgtcttca acttttagac tttgatttat agtatcacaa aactcattta
    69121 tttcattaat taatttttct gagcttttat ttactgctac ttttgcttga atgcaaccca
    69181 gcttagcatt aggccatctt tccttaagtt tgtcatcaat acttacatct atcatactta
    69241 aacaactcct atacactaaa tttttattaa agtttttatc atctatacat tatagtacta
    69301 ttcttttgtt aataacatgt ttattagcaa aataaattta tttttaatat aatttttata
    69361 aaatacaatt attttttgct ttatattaga caatacattg ataaaataaa aaaacactaa
    69421 ttatttagca atgatttatt aaaatcaagc attcataata gtgctttata taaaaatcaa
    69481 attttgtttt aaagtaaatc taaaaaattc ttagctggca ttggtcgtgc aaaataaaat
    69541 ccttgaacta aatcacatcc aaattgtttc attaactcag cttgttcttt actctcaaca
    69601 ccttctataa ttacaactaa atctagtgat tttgctagag acactattga ttttgctata
    69661 ttgcgacttc tctcatcatg ttcaatgcca ccaagaaaag acttatctag tttcaatata
    69721 tcaattggca tatctcttaa acaactaata gaagaatatc ctgtgccaaa gtcatccata
    69781 gaaatagaaa atccatgctt cctcaattca tttaaaacat tctttaaaat atttaactca
    69841 ttaatagcca cagtttctgt tatctctatt tcaatgaaat ctgaacttag attatacatt
    69901 tgtattgttt tattgataaa ttttacaata tctggttgat acaactcgac tctagataaa
    69961 tttactgaaa ctggaactat ctttttacct tgctttttcc attcagataa atcattgcaa
    70021 accctttcaa atacaaatct cccaattttt aaaatcaact tacttttctc tgctattgga
    70081 ataaatactg cgggacttat aaaaccatgt tctggatgat tccatcttat tagtgcttca
    70141 ctccctatca tttctccagt tacaagtgaa aacttaggtt gataatacac ttcaaattga
    70201 ttttttacca atgctatttt tatatcatct aaaatcattg attcttcact aagcttattc
    70261 ctaacatctt cattataaat agcataattt atatttttac ctttagcaac agacctagcc
    70321 atatttgctt tatttacagc cttttgaata tctacttcac cttcttcaac aaaataaata
    70381 cccacacaca gagagatttc aaagactata ctcaaattac tttcaatatt acttcgtata
    70441 ttatcaagtt ttcttataat tttatttctt ccatttttat aatcacagaa aattgcaaaa
    70501 taatcacttg aaagtcgtgc atatacctca ccttctgtta attcttcttt taaagctttt
    70561 gctattgtaa ttaataaatt gtcaccaaat tcatatccaa aagtatcatt aatcatctta
    70621 aagttattaa tatcaaaata tactataata taatttaaag atggtttatc taaaagatac
    70681 ttactacact ctctataaaa tttattttga tttcctatat atgtaacttt atcttcataa
    70741 gccaaactta atatttcttt ttcattagat ttttttatat aaattatata aaaaataatt
    70801 gcaacaaata ttaatacaat agataacact gcatatacag ctctgttgat tatttgtttt
    70861 gaattactaa atacaacagt ttctggaatt gaaaaaatta aattccagtc ttttatataa
    70921 ttattttcac tattcccatt atccatagtg gcatacccta aatatcttct ttcttccaac
    70981 atatttaata cagttgcacc tgttttattt tcttgcaatt ctttttttaa tctatttact
    71041 tcttgaatat cattatcctg ttctaacaat ttataaatat tttttgatag gctatcccct
    71101 tgtggatgaa ggataattgt accatcattt tttacaacat aagatatact attattttca
    71161 taaaaagata tattaataag tttcatcaga gtattgatat tgtaagtaca atataaaact
    71221 ccaactactt tatcattatt taataatggt actgaaatag caatcttctt tatagactta
    71281 tttacttcat caacatatgg acttgaaaca tacttcttac ctttaattga gtttttgaaa
    71341 aattctctat ctttcactga aaacttatca cctttggtat tttcagcatt accatttata
    71401 tcaactattg ccatagtgta aaatttttga ttttccattt catttaaaag atttttcatt
    71461 ttttcctcat taggtatatc tggatttgaa gcataatagt tagaaaaaat atttaaaaca
    71521 tctaaatctt tttcaatttg tctttgtgct agttgagagc cttgtactgc aacctctttt
    71581 atatgtattt ttgcctctga cataagaaga tgttttgtat cttctatata gatatacccc
    71641 attgtcaaaa ataatgatgt taaaaaaaga attgtaatta atgtaaaact tttatttctc
    71701 cctgtcattc gactcctccc ctaattagtc aatacttcat atagataata tatccttttt
    71761 ataccaattc atcatttata ttgtatataa ctattcttaa atgttacaca taaaatcaac
    71821 ataataacca taaaaacatt cttttattat tataataaca ctatttaatt gaattttaaa
    71881 ttaaagaata aaaactatcc attttctcaa acaaaaaaac tcttatacaa taaaactttg
    71941 ttttatgtat aagagttttt attttatcta ttttatagca aacttatctt ccataatatg
    72001 aatttaaata catatctttt atttcattca taagtggata tcttggattt gctcctgtac
    72061 attggtcatc aaatgcttga attaccattt catctattga atccataaac ttagattctt
    72121 caactccaaa ctcttttata gatttaggaa taccaacttt aagttttaaa tcttcaatag
    72181 ctaccaataa actttggaat ttctcttcat cagaatttcc ttttaatcct aaaaatgaag
    72241 ctatttcagc atatctcttt aatgcttctg gatatttata ttgagagaat gctcccattt
    72301 tctttggtgc atctgaagaa ttaaatttca taacttctgt tatcaaaaga gcatttgcaa
    72361 caccatgtgg tacatggtgg aaagcaccta atttatgagc catagagtga caaacaccca
    72421 aaaatgcatt tgcaaaagcc atacctgcca ttgtagaagc atttgccatt ttttctctag
    72481 cttctgggtc ttttgcacca ttatcatatg cacgtggaag atattcaaat atactcttaa
    72541 tagcttgtaa agctaatcca tttgtataat cggttgcaag catagataca tatgcttcta
    72601 atgcatgagt taaagcatct ataccagaag cagatgttaa gctttttggc atattcatca
    72661 tcatatcaga atcaactata gccatatttg gcatcagttc ataatctgct aatggatatt
    72721 ttactcctgt atcttggtca gttataacag caaatggtgt tacttctgaa ccagtacctg
    72781 ctgatgttgg tatagctaca aaattagctt tttcacccat tttagggaat gtatataccc
    72841 tttttcttat atccataaat ctcatagcta ggtcttggaa atctacatct ggatgctcat
    72901 acaatgtcca cattatcttt cctgcatcca ttgcacttcc tccacctata gatattatta
    72961 catctggatt aaattctctc atagctttag cgcctatctt agcacattcc aaagttgggt
    73021 ctggttctac ttcaaagaat gtagtgtgct taatattcat ttcatctaat aaatcagtaa
    73081 ctgctttagt atatccatta ttgtaaagga atgtatctgt tacaataaaa gctctcttct
    73141 tattcattac atctttaagt tcttttaatg ctactcctaa acaacctttt ttgaaataaa
    73201 ctttttctgg tgctctaaac caaagcatat tttctctcct ctcagctact gttttaatat
    73261 taagtaaatg cttaactcct acattttcag atactgaatt tccaccccaa gacccacatc
    73321 ctaaagtaag agaaggagct agtttaaaat tatataagtc ccctatacca ccttgagaag
    73381 aaggagtatt aattaaaatt ctacaagttt tcatggctga agtaaactta tcaagttttt
    73441 ctctttgatt tacatcatca atatataatg aagatgtatg cccaaatcca ccatcttcta
    73501 ctaatttttc tgcttttcta actgcatcat caaaatcact tgccttgtac attgcaagta
    73561 ctggtgaaag tttctcatga gcaaaagctt cttctatttc aactgattct acttcaccta
    73621 ttaaaacctt agtatctact gggacttcaa agcctgcaag ttttgctata gtacaagctg
    73681 tttgcccaac tattttagaa tttaatcctc catttactaa aattatattt cttatttttt
    73741 ctgtttcatc tttatctaat aaataagcac cacgctcttt aaattctttt ttgacttcat
    73801 tatatatatt ttctaatact ataaccgatt gttcagaagc acatatcata ccattgtcaa
    73861 aagtcttaga tacaagaatt gagtttacag cagtttttat atctgcacta tcatcaatta
    73921 ttgctggagt attacctgca ccaactccaa tagctggttt tccagatgaa tatgctgatt
    73981 taaccattcc aggacctcct gtagcaaggg tcaaatccac ttctttcata acagttgtag
    74041 ttaattctaa agatggctca tctatccatc ctattatacc ttcaggagct ccagctaaaa
    74101 cagcagcctc aagaacaact tttgcagcct ctatagtaga attttttgct cttggatgtg
    74161 gagagaaaat tattccattt ctagttttta aagctaaaag tgctttaaag atagcagtag
    74221 aagttggatt agttgtaggt acaactgctg caataacacc tattggctca gcaattttag
    74281 tcataccaaa tgcttcgtct ttctctataa caccacaagt tttagtttct ttatatgcat
    74341 tatatatata ctctgaggca taatgattct ttataacctt atcttcagca attcccatac
    74401 cagtttcttt ttgtgccatt atagctagag gaactctttg cttattagct gccaaagatg
    74461 ctgctaaaaa aattttatct acctgctctt gactatatgt agcaaatatt ttttgagctt
    74521 ctctgatttt tgctagtttt ttaactaagc tttcaacgtt atttacagta tctaaaccaa
    74581 ctgacataac ttcattttgt tttttatttt ctattattac ttgttcttta tgagccattt
    74641 taacaacctc ctgagatagt tttttattta ttatgttaat ttattaacat tatagcattt
    74701 actttttcta ttttcaagtt atttttttaa catt
//
LOCUS       DAGSWI010000002        68147 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.002, whole genome shotgun sequence.
ACCESSION   DAGSWI010000002 DAGSWI010000000
VERSION     DAGSWI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 68147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.002"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            38..676
                     /locus_tag="MJF80_000064"
     CDS             38..676
                     /locus_tag="MJF80_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278753.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQNYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(1170..2930)
                     /locus_tag="MJF80_000065"
     CDS             complement(1170..2930)
                     /locus_tag="MJF80_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBZ0278754.1"
                     /translation="MQEVEVIKAKNVLSRQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSQYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKPQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(2993..3136)
                     /locus_tag="MJF80_000066"
     CDS             complement(2993..3136)
                     /locus_tag="MJF80_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBZ0278755.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            complement(3455..3694)
                     /locus_tag="MJF80_000067"
     CDS             complement(3455..3694)
                     /locus_tag="MJF80_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0278756.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            complement(3699..4115)
                     /locus_tag="MJF80_000068"
     CDS             complement(3699..4115)
                     /locus_tag="MJF80_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBZ0278757.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGVMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            complement(5462..5629)
                     /locus_tag="MJF80_000069"
     CDS             complement(5462..5629)
                     /locus_tag="MJF80_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278758.1"
                     /translation="MNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKKDTKESYK"
     gene            complement(5642..8407)
                     /gene="mgtA"
                     /locus_tag="MJF80_000070"
     CDS             complement(5642..8407)
                     /gene="mgtA"
                     /locus_tag="MJF80_000070"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBZ0278759.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            complement(8573..8884)
                     /locus_tag="MJF80_000071"
     CDS             complement(8573..8884)
                     /locus_tag="MJF80_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBZ0278760.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            complement(9131..9808)
                     /locus_tag="MJF80_000072"
     CDS             complement(9131..9808)
                     /locus_tag="MJF80_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBZ0278761.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            complement(9946..10449)
                     /locus_tag="MJF80_000073"
     CDS             complement(9946..10449)
                     /locus_tag="MJF80_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBZ0278762.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            complement(10465..13128)
                     /gene="mgtA"
                     /locus_tag="MJF80_000074"
     CDS             complement(10465..13128)
                     /gene="mgtA"
                     /locus_tag="MJF80_000074"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBZ0278763.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARHSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGISTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            complement(13221..13430)
                     /locus_tag="MJF80_000075"
     CDS             complement(13221..13430)
                     /locus_tag="MJF80_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278764.1"
                     /translation="MVKVKTKKKFVSTHMCQGEMPIYVRERYEKMLAVSIRAPNLEGM
                     VENFDKDLSHPNETDLIELKNAISK"
     gene            13380..13716
                     /locus_tag="MJF80_000076"
                     /pseudo
     CDS             13380..13716
                     /locus_tag="MJF80_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13915..14817)
                     /locus_tag="MJF80_000077"
     CDS             complement(13915..14817)
                     /locus_tag="MJF80_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBZ0278765.1"
                     /translation="MFKKNKKSTENVKEKKVRTVKVGTHKKTVIALWVVLIESVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIEARTQAING
                     YLTKELQDLNVDTIRTDIPTSSTITDVIVWSIEQSGTDTFSATYEVDQQIEEGEQTTA
                     VKATYTVKVHVDDDGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKDSNWKIIG"
     gene            complement(14833..15840)
                     /locus_tag="MJF80_000078"
     CDS             complement(14833..15840)
                     /locus_tag="MJF80_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBZ0278766.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFLGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVHVLLAIIQVESGGTAEDVMQSSESLGLLPNS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSVGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVAHFDNATAQAIM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNKMYHAGDPIGYADLSSSYWQQHLIGAGRVK
                     E"
     gene            complement(15843..18047)
                     /locus_tag="MJF80_000079"
     CDS             complement(15843..18047)
                     /locus_tag="MJF80_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0278767.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIITVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRSERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARHRKTDGSATTGATRPHERPA
                     TASTVPKTSVEKMQEIKRPATAPTSKASEPVKTNVIKERPLSSGASDMKATQSAQPVH
                     RQNVEKVVSQETRQNYTKDRRTKVQQTQTVQKNQQTTEKNRNIVTKKGQKKK"
     gene            complement(18047..20497)
                     /locus_tag="MJF80_000080"
     CDS             complement(18047..20497)
                     /locus_tag="MJF80_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0278768.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(20475..20873)
                     /locus_tag="MJF80_000081"
     CDS             complement(20475..20873)
                     /locus_tag="MJF80_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBZ0278769.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            complement(20991..21494)
                     /locus_tag="MJF80_000082"
     CDS             complement(20991..21494)
                     /locus_tag="MJF80_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBZ0278770.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            complement(21512..22015)
                     /locus_tag="MJF80_000083"
     CDS             complement(21512..22015)
                     /locus_tag="MJF80_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBZ0278771.1"
                     /translation="MQETRVLVETRGTTGEETISYWFDLPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSSDMQEEYVSLLTVYENLEALYICR
                     NVITVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(21934..22230)
                     /locus_tag="MJF80_000084"
     CDS             complement(21934..22230)
                     /locus_tag="MJF80_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBZ0278772.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     EDFSKYVFGMSYQTIKDVRKQQKGREEQCRKQGCWWKREEQLAKKQYHTGLTCR"
     gene            complement(22313..22954)
                     /locus_tag="MJF80_000085"
     CDS             complement(22313..22954)
                     /locus_tag="MJF80_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
                     /protein_id="HBZ0278773.1"
                     /translation="MEYKYTKIDLEKWNRGKLFQSYIDDMRIVMSLTVDIDVTNLIEF
                     SKSHDLKFYPAMIWVVSKVVNEHDEFKYSWDNKGNLIKWDYISPSYAEFHKEDENFTK
                     MVTDFSDSIFEFHERFMLDREKHKMERAFVANQPLNFFDVSCLPWVKYNHFDVHVFDE
                     GKFLAPVITWGKYELERERYIMPLTMNIHHAVADGFHLSRFFNEVQELINSIS"
     gene            complement(23162..23383)
                     /locus_tag="MJF80_000086"
     CDS             complement(23162..23383)
                     /locus_tag="MJF80_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278774.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(23384..23518)
                     /locus_tag="MJF80_000087"
     CDS             complement(23384..23518)
                     /locus_tag="MJF80_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBZ0278775.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSIGKEADYEMKQLEESEDN
                     "
     gene            complement(23531..24727)
                     /locus_tag="MJF80_000088"
     CDS             complement(23531..24727)
                     /locus_tag="MJF80_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBZ0278776.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LVDGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLNEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQATLSEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(24911..26305)
                     /locus_tag="MJF80_000089"
     CDS             complement(24911..26305)
                     /locus_tag="MJF80_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0278777.1"
                     /translation="MRKIWNKGHRIRASDKHLVYHFSIGTLLFVFVAVLLLLNIKQLM
                     RTDWEHFSLLEKGLTLSPYNLITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNSFYEGMVQRSEEMKQHSNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLKTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            complement(26372..26986)
                     /locus_tag="MJF80_000090"
     CDS             complement(26372..26986)
                     /locus_tag="MJF80_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278778.1"
                     /translation="MSEKLLDLLKIFLEKYLVPTVIAIVFSFIAYYVTPTDNKLLVKF
                     TIWGYAVFLFCVWFLIIELVIWLVQKIQYHNYSKGVEQRNKQRQAEEWQQSLEFLWTD
                     VDNLSSSDYKLLLRFIKNENQPYCSSGTPMGHCLLNSDWVHKTVSKPAKRVLIKSNQR
                     TSSKAIPIPVYETISETYQYILREDIYQMLKYSYEKYGKISHFK"
     gene            complement(27077..27460)
                     /locus_tag="MJF80_000091"
     CDS             complement(27077..27460)
                     /locus_tag="MJF80_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBZ0278779.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNAGNPQNHTPQGQTKK"
     gene            complement(27476..27802)
                     /locus_tag="MJF80_000092"
     CDS             complement(27476..27802)
                     /locus_tag="MJF80_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBZ0278780.1"
                     /translation="MEMKYVVPDMTQSFGTLEFAGESEPVFERDKNNRRVLARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDMGHTDYVLVADDI
                     VAVEEK"
     gene            complement(28041..31085)
                     /locus_tag="MJF80_000093"
     CDS             complement(28041..31085)
                     /locus_tag="MJF80_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBZ0278781.1"
                     /translation="MKKMLKRLCTGFLALATIVTALPTTPVHAESKQYWTESKERVGI
                     VEKVMNDGSIGSTFNEGMMKVEGETAYCIDINTDFKNGYKTRTDASSRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSISGATYGVFSDKDCTKQLATLTTDENGNTNVVEVKAGTVYIKELSAPAGYKVDKT
                     VYPLTIKAGETATLKVSDTPKVTDTLIELFKIDMETQKDNPQGNASLAGAKFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDETTHYITKLANAYKVSGDSFYMQDGKAVLPL
                     GTLTVEETKAPKGYLLDGAYMQAGDKSEQIKGVYVTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNAVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGNFRIVESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSADWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEEMCCDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFVADEEEMKVEISYTFNASALGGKNPVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LKGLEVGTKYKLVGWQMLKAENAELLINGKRVESDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDKPKKVTEHKDIEDEGQTITFKEKPEEPEKPETPQTPEKPN
                     RPSDSPKTGDSTNVMAFIVMLFASAGGLAGTYLYKRRKMKKS"
     gene            complement(31330..32694)
                     /gene="rlmD"
                     /locus_tag="MJF80_000094"
     CDS             complement(31330..32694)
                     /gene="rlmD"
                     /locus_tag="MJF80_000094"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBZ0278782.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIQDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSENLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETCVLLQ
                     RRTM"
     gene            complement(32869..34629)
                     /gene="pyk"
                     /locus_tag="MJF80_000095"
     CDS             complement(32869..34629)
                     /gene="pyk"
                     /locus_tag="MJF80_000095"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBZ0278783.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(34681..35640)
                     /gene="pfkA"
                     /locus_tag="MJF80_000096"
     CDS             complement(34681..35640)
                     /gene="pfkA"
                     /locus_tag="MJF80_000096"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBZ0278784.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(35849..39418)
                     /locus_tag="MJF80_000097"
     CDS             complement(35849..39418)
                     /locus_tag="MJF80_000097"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBZ0278785.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMNSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKVLEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(39575..40522)
                     /gene="whiA"
                     /locus_tag="MJF80_000098"
     CDS             complement(39575..40522)
                     /gene="whiA"
                     /locus_tag="MJF80_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBZ0278786.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(40539..40964)
                     /locus_tag="MJF80_000099"
     CDS             complement(40539..40964)
                     /locus_tag="MJF80_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBZ0278787.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(41033..42139)
                     /locus_tag="MJF80_000100"
     CDS             complement(41033..42139)
                     /locus_tag="MJF80_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBZ0278788.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLKNGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVEHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(42151..43008)
                     /gene="rapZ"
                     /locus_tag="MJF80_000101"
     CDS             complement(42151..43008)
                     /gene="rapZ"
                     /locus_tag="MJF80_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBZ0278789.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(43039..43752)
                     /locus_tag="MJF80_000102"
     CDS             complement(43039..43752)
                     /locus_tag="MJF80_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBZ0278790.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(43756..44670)
                     /gene="murB"
                     /locus_tag="MJF80_000103"
     CDS             complement(43756..44670)
                     /gene="murB"
                     /locus_tag="MJF80_000103"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBZ0278791.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(44943..45713)
                     /locus_tag="MJF80_000104"
     CDS             complement(44943..45713)
                     /locus_tag="MJF80_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBZ0278792.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            46031..47512
                     /gene="cls"
                     /locus_tag="MJF80_000105"
     CDS             46031..47512
                     /gene="cls"
                     /locus_tag="MJF80_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBZ0278793.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            47830..48327
                     /locus_tag="MJF80_000106"
     CDS             47830..48327
                     /locus_tag="MJF80_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBZ0278794.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            48344..50230
                     /locus_tag="MJF80_000107"
     CDS             48344..50230
                     /locus_tag="MJF80_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBZ0278795.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            50253..52034
                     /locus_tag="MJF80_000108"
     CDS             50253..52034
                     /locus_tag="MJF80_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBZ0278796.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(52174..53970)
                     /locus_tag="MJF80_000109"
     CDS             complement(52174..53970)
                     /locus_tag="MJF80_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBZ0278797.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(54184..55113)
                     /locus_tag="MJF80_000110"
     CDS             complement(54184..55113)
                     /locus_tag="MJF80_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBZ0278798.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(55113..56930)
                     /gene="uvrC"
                     /locus_tag="MJF80_000111"
     CDS             complement(55113..56930)
                     /gene="uvrC"
                     /locus_tag="MJF80_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBZ0278799.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(57189..60014)
                     /gene="uvrA"
                     /locus_tag="MJF80_000112"
     CDS             complement(57189..60014)
                     /gene="uvrA"
                     /locus_tag="MJF80_000112"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBZ0278800.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(60033..62003)
                     /gene="uvrB"
                     /locus_tag="MJF80_000113"
     CDS             complement(60033..62003)
                     /gene="uvrB"
                     /locus_tag="MJF80_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBZ0278801.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(62190..63452)
                     /locus_tag="MJF80_000114"
     CDS             complement(62190..63452)
                     /locus_tag="MJF80_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBZ0278802.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            complement(63515..64819)
                     /locus_tag="MJF80_000115"
     CDS             complement(63515..64819)
                     /locus_tag="MJF80_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBZ0278803.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(64850..65710)
                     /locus_tag="MJF80_000116"
     CDS             complement(64850..65710)
                     /locus_tag="MJF80_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBZ0278804.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(65754..66623)
                     /locus_tag="MJF80_000117"
     CDS             complement(65754..66623)
                     /locus_tag="MJF80_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBZ0278805.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(66607..67677)
                     /locus_tag="MJF80_000118"
     CDS             complement(66607..67677)
                     /locus_tag="MJF80_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0278806.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
BASE COUNT        20339 a        13643 c        10020 g        24145 t
ORIGIN      
        1 tagaaaaaat caaaagaaat attagaagaa atggatattg agtaatcaaa gaaaagattt
       61 tgatattaca aatgatatgt ttcaagaaag cattcctatc acaacaaaaa ttattcttga
      121 agacatgcct tcaaatgatg aattaaatca tgttttttca aaaggttgtg aacgaaaaat
      181 gaaaaagaga aaaattgtat taatcacact actattaata ggtgttttgt tattaggaag
      241 tatcctatat aatctttttt taggaaaaac agctaatatt tcaatgctaa aggaatcttg
      301 gaattttgat ataccaatac ctaacaaaga aattgaagta tttgatactc aagatagtat
      361 taatggagat ggacaaaatt attttattca aggattttct gaaaaaaatt tcaaaaaagt
      421 ttttaattta aaaggtggta tagttgtaag caaagataat attaatgaaa tagaaaaata
      481 tattgataaa tttaaaagag attctgttaa cattaataaa agtaataaaa ataaaattga
      541 agaagatttt aaaaaatata agttagaagt taaaaaagat gataagtata tatataagag
      601 aaattatgag aactacgtag ttttaattat aaaaaaagat gaacaaaaat tatacagttt
      661 aatatggaat cagtaggtac atatgtatta ttaagaatta taaataataa aaataaattt
      721 tagtcaatat acaaaaacct tttaaattgt ctggaaatca cctcttcatt tgttataaaa
      781 acatacaaca aatgaagagg tgattttatg gttttaaaaa aaataattat aagtggatat
      841 gtgctttcac atgtttatca acatttgtaa tgcctatatt tgctaatgaa ttataatata
      901 aaaatattaa ataatattca actatagatt atgtaaatta ttctatgaaa aaaatgagtt
      961 gtataaaatc tggctactgt agattgacga tttcttttaa aattacaatt ataataagtt
     1021 ttatagaatt aaaaataaaa ctttagagat ttttgataac attgtagact aaatttgcca
     1081 ccgaaatcgc tttgcagtgg caaatcggtg gcaaatacaa tatattatta gtatataatc
     1141 tatataaaca ttactattgc ttgattatct tagctacagt ttccacatgt gtcgttacta
     1201 ctatcggaac acattttatc aacctcgttg ctgtcatgtg aacacaattc attagtccta
     1261 tgccgtcctc ttgcattttt cctttgtggt ttaaatttag caccatctac gctattcttc
     1321 aagcctgttt tataaacaaa ggtcaactgt gcagggtcaa catttccgtt ttcatcaagt
     1381 ccacctacaa ttactttttc aacaatgctt tcaaacacat agcggtcaaa tttatcaaga
     1441 acctcattct gttccagagt tttcttaaac tctttcaaac gtttttttat gtctttctca
     1501 ttatcagaag tttcttgtaa cttttgtctt tcttcaacaa gctgttcttg tttacttact
     1561 aaatcagcat atttactttc gtaggtttcc ttatcaataa tttcttccag acgcatatcc
     1621 acaagtttac tttttttctt ctctaatgcg tctatctcct tttccgcttt tgcaagttgt
     1681 tttgacacca cactactact aagagtatcg tccattctct gcaaaaattc ttccagaaca
     1741 tctttattat catcacataa taaacgataa ctttctacaa atgcttcttc aatagctgtt
     1801 tctggaatac ctttgctatg tttacaaaat ttctttcctt tcttcgtagc cgttacacat
     1861 tgccatataa ccttactgta ttgtgagcca ctgtgccaat tacgtcttgt taaagtacca
     1921 ccacaaaagc cacattctag catacagcta aatgcgtatt tcctgctata cttctcacgc
     1981 ttaccgctgc ctaccgttgt tcggttctta tttctccgat taagaatttc ctgtgctttt
     2041 tcaaaaactt cttcactaat aatcggctca tggtgatctt tcatataaaa tttatcttct
     2101 tcacccatgt tttcaaggcg acgtttggaa atcggatcaa cagtaaaagt tttgcctaag
     2161 agtatatcac ccttgtattt ctcattctta ataattccaa taaccgtaga tggtgcccat
     2221 gtggaactcc catacttcgt tttataacct agactttcaa gctcctgtcc aataacagaa
     2281 ccaccagcac catcaacata acggttgaaa atatatctga caatctctgc ttcttcttga
     2341 tttacagtta ttgtcttatc ctctgggtga taatcatatc caatacaacc ttgaaaacct
     2401 accagctcgc cgcgcttcat tttcattttc aacccttttt ttacattggc tgaaatgttc
     2461 tcgacttcct gctgtgcaac agaactaaga acaacaagca ataactctcc atccattgta
     2521 agtgtattga ttttttcatc ttcaaaataa acggcgatat ttctctcttt tagcatacga
     2581 acatatttca atgtgtctaa agtatttctg gcaaatcttg atatggattt tgtaaatacc
     2641 atgtcaattt caccattcat acagtcattt atcatacgct gaaaatcttc tcgttttgtt
     2701 acttgtgtcc ccgtaattgc ttcatcagca taaatgtcag ctaacaccca ttccttgttt
     2761 ttcttaatca tgtcagtata atactgaact tgtgatttat aactgtttaa ctgatcttcg
     2821 ctatctgtac ttacacggca atatgcagct acacgcaaac gctcaatggc ttttccacgg
     2881 gcttgtcttg acaaaacatt ttttgctttt attacttcaa cttcctgcat agtacacctc
     2941 cttattttgt gttcctttca tattgttatt gtactacttt aaaaaacaca attcaagcag
     3001 ttatgtcaga aagcacctta tagtctttca tcagcctttg ttttacaaga gtgtattctt
     3061 tttcagaaat cagtttacgg gcaagcagtt gttttaacat agcaatttgc atactatacc
     3121 taattatatt attcattaaa accctccttg cttttttatt tatggaagta atcagacggc
     3181 ttcggcagct ccactggact ttcaccattc ccattctgat gggtgaaccg tgtcatacgg
     3241 gtgtatcatt attcagatat acgagtcatg gcagcaacct ttccaaagat cgcttgcggt
     3301 tcactggcat aatcgctcgc tgctttcccc ctttcagtgg tcgtggcgtt ccagtccgct
     3361 ggctcgtcgt atatcaaacg tatctgatta ctgtattcac ttgtcaaaga acggaaaaga
     3421 gttaaaatga ggtggtagat actctctgtt atatttactt cactttgact tcaaacccta
     3481 agattacttt aatcaacgct gcccgtatcc tgcctttcag ttccatatca agcacgatat
     3541 acatattccc gtgttcgtca tagaacgggc gtaggctggc ttttgatatg taagcgtcat
     3601 aatggttcaa aatcttttca attgccacct cgttaccgtc agctgcagag ctgatggtcg
     3661 agaatgatgg acactttttt gtagacttca tcatgttttt attcctcctc gtacatatcc
     3721 ttaataagct ttaatgtgtg cagcctgttt ctacatacgg aaaatcgttc catttccata
     3781 acctctgcaa tttcagcgtc ggtcatttca agaaagtagg acataagcag aatgttacgt
     3841 cggctttctg ttaattcttt gatagcttca cataagcgtt catcatagat atggacttct
     3901 gaaccaaata catcaaatga cgtaaactca acgcaatact cgtccatgac accgatctgg
     3961 tttaactcca tttcagataa atcacagaac ggcgtttcac gtcttgcacg tctgccaagc
     4021 tctcggtcat agttctttac agttcgtcct acaaccttgc gagccagaca atcaaattga
     4081 agtctgatag cgccctcgaa agaagatggt ttcataatct cacccccttt ccagcgaaag
     4141 ttgctaaagc gaaaagactt ctacctcttt tcgcactaac actcaccgtg aaaggtggga
     4201 tctgataccc aaccacaaaa attttttgaa aaagttttat gcagtaaaaa agcacaaggg
     4261 caatacagac gtactgatct tgtgctaaac aaattattcc tgttatatgc tgtaaaaaac
     4321 cacatatagg agcattatgt tctgtagaat ttaaatgttt ttttaatgaa ctaccgatgg
     4381 tcgaaagttg ttgaatatgt aagtgtttca tacggctacc cctagagccg ataaaacaag
     4441 accatccagt aatttgtcca acgttaggca aaaataaaac ggcggctata taaccgtcgt
     4501 cttattcaaa agttcaaaaa gaactaaact aaaccctcta aagcagtatg tatttataat
     4561 cagtcttgca aaataacaaa gtagaattga aatattaagg tagcaatcct tgtgcatcta
     4621 ataaacttat aggggttaca gcagaccacg gaataatatt ttgtatactt tcagatcaat
     4681 atagtccaac aaaaccaatt cccgatattt agtaactaaa aagtaaacta gagtagaaaa
     4741 catttctttg caaaacacaa ataataagta atggactcat tgacaataga ttgagaaatc
     4801 cagtttttta gacttagata gattatctca cttgtaaatg atttaagttc tttgacttca
     4861 tatatagcgg tataatctgt caacgtgatc attacaaacc ataatgccta tataacatac
     4921 tttccaagca aaaactttat tctattagtt ggggaataca aaatatttat cattgtgcgt
     4981 tcaacatatt ctttttgtat aaaaatgtaa ttacaaaaca ccactcaata taagatagat
     5041 aataatttga ataggaatta tcaatctacg taaccattca ttacctgtaa ttgttgttac
     5101 aatagatttg ggattctgtt tccattacat aattttttta tccttctttc agagatctcc
     5161 tcattttatc gaagcttctt ctacagacat acattccata ataaaatcta cctttgaata
     5221 ttatttcacg atttcgttat agacgcgtaa acaaacaata aaaaacgaaa tttcttttaa
     5281 gtaccataat ttttatatcg aaattacaat tatccactta tatacagcat ttataggtaa
     5341 ttcaatgata aaaaacacat actttactac taaattgcca caattgaatg cgccttgata
     5401 tgatagggag attccacaat aaaaaactgt gcatctcccc tgattcattc atactgatat
     5461 cttatttata agactctttt gtgtcttttt ttggtttcat ggaccaaaac actacattca
     5521 ttacaataac aatcagcaac acaactgcca tagccaccca aaagccgata ttaagaccaa
     5581 gccattcggt agttccaaaa agggtcatcc aaagttcttt ccagttcatc attccacctc
     5641 cttaaagcag ttctccgaaa tgacggacat aggctttttt taaacttgtt gccaaagtca
     5701 tatacagcag aatacatgga atcagataag caaagtatgc tgcaggcaat gcaacaaatc
     5761 caagcatagt tccaaacaca gtaaacggaa taatggtcag aactataatt ccagtcattg
     5821 ttagcaaagt caccggagct gatgcacgac tctgaacaaa aggcagcttt ggagtacgaa
     5881 tcatgtgaat gactaatgtc tggctccaca tagattccac gaaccaccct gcttggaaca
     5941 tcccgatata ctgtgcctga agcattgcca gttctgcacc gctgaaatga gcaggcaaat
     6001 cattaaataa tacaccattc gatacgaaca atggacaaaa tacaaagtac ataaaaatat
     6061 aggttgtaaa atcaaagata gaactggttg gtccaatcca gatcataaaa cttccaacac
     6121 tagaagcgtc ccatttacga ggtttggtaa taaactcctc atccacatta tcccaaggaa
     6181 ttgcagtaca ggataaatca taaattaagt ttagaaatat aagatgaaca ctcatcattg
     6241 gtaagaaagg taacaaagca gatgcagcaa gcactgagaa catattgccg aagttggatg
     6301 aagcagtcat tttaatgtac ttgatcatgt tggcatatgt ctttcgtccc tcaataatac
     6361 cctgttccaa aaccatcaga tctttttcca atagaataat gtcagcagat tcttttgcca
     6421 cgtccacggc ggtatccacc gaaataccaa tatcggcggc tttcatagct gcagcatcat
     6481 taataccgtc acccatgaaa ccgaccgtat gaccattttc acggagaaca gaaaccacac
     6541 gggctttctg atcaggggtt agctttgcaa atacatctgt agactctgcc actcttgcaa
     6601 gctctgcatc gcccatttgt tccaaatcag aaccgagaag catattgcga acctctaatc
     6661 ccacctgctt gcagatagtt cgagtcactt tgtcgttgtc acctgtcaaa attttggtta
     6721 taacaccatg gtctttcaga gctttaatag catccgcagt agattctttt ggaggatcaa
     6781 ggaatgccag atatccaatc agtaccatat cacactcatc tttaacacca aatgcgccga
     6841 ttggggaagg attactcttc tgtgcaatag ctagtacgcg gaatccctta tcgttaagat
     6901 tgtccactgt ctccagaatt ctgtaacgaa cgtcatcagt cagaggctgc actcctccat
     6961 tacactctgc aaacgcacaa atagagagca tttcttccac agcacctttt gttaccatct
     7021 gggtcttacc cttattatcc tgtaccactg tagtcagacg acgacgtgtg aagtcaaaag
     7081 gaatctcatc tactttcaca tagttttcag acaggtcaat gagacgcgaa tctgcggctt
     7141 cctcttcctc agtcttatgg ataattgcca aatccatcag gtttttatag ccagtctgga
     7201 agtaactatt gaggtaagcg tgacgcagta ctcgcgtatc gtcctcgcca tttacattca
     7261 gatggtattc cagcacaacc ttatcctggg tcagcgtgcc agtcttgtca gtacagagaa
     7321 tatcaatagc accgaagttc tgaatggaat tgaggttttt aacaattgtc tgcttcttgc
     7381 tcatggaaac agcgcctttc gcaagacagg ttgttacaat catcggcaac atttctggtg
     7441 tcaaaccaac agcaatagaa acgccgaata agaaggccgc caaccaatcg cccttggtga
     7501 taccattgac aaagaatacc agcggaacca taatgagcat aaaacgaatc aatacccaag
     7561 aaacggcatt gacacccttg gtaaagcttg tttcaaccgc ttctccagca actgcggacg
     7621 ccatagagcc aaacagagta tgatcgccaa cacaaacaac aacagctgtt gcgctaccag
     7681 aaattacatt actccccata aaagcaatgt ttgtgtaatc agtcacgctt tctttctgag
     7741 cacttacatt gggtgttttt tcaataggtt cactctcacc agtaagactt gcctgactga
     7801 caaacaaatc cttcgcatcc aaaatacgaa catccgccgg aatcatatct ccagcagaaa
     7861 gatgcacaat atctcccacc accaaatcat ccataggaag ttcttttttt tcatagtttc
     7921 tacgagtaac tgtacaagtg gtcgtaatca tggctaacaa tttctctgct gcatttccgc
     7981 ttctggattc ctgtacaaaa cgaagcgtac cggaaatgaa taccattgtt aaaatgatta
     8041 caacagtcag ggaatcaaag tcctctggca cactgccaaa cagagaaaaa tacggaaaaa
     8101 tcatatctgt aattgtggat actaaagcca gacaaaataa aatggcggta aatggattga
     8161 taaatgcgcc aaccagtcgt tttgccagag atttcttttt ttcatgagtg actttattgg
     8221 taccatattt agcacgactt acggatactt catctacttc taagccacga agtgtggtat
     8281 gaagtttttt taaaacttct ttaataggat tggttgccgc aaattgaatg cggcggttct
     8341 gttcgtcacg gatgactgct ttttctacag tctgacgaat agccatacga ttttcttttt
     8401 tgttcattgg tcttacctcc ttgaattttc ataggggcaa ggaatattat atgagcgaca
     8461 cggaatctcc aaattctcat ataatattct ctgccttcgt ggactgacat cagagtccat
     8521 gtctttcacc tcacaatctt aaaaaaatat ctatgtgaaa cctcaatgtg gattattggt
     8581 tgcgatagcg gacacttttt tcgatatacc agacatcaaa gccggcaaca atcagccatg
     8641 cgattattgc aaatacaaac cagtggtttg aaaatcccgg caaaaatcct ttcattaaga
     8701 atataatccc atttaacgca atcatttctc caacattacg acaaagcggg acaatattaa
     8761 ttttatcttt ctcctcctgc ggcatatttt tccacaggga aagatgacga tatccttttc
     8821 caaaggccaa ccaaaagcct gcaagagtaa ataaaattcc aaaaaagata catgtaaaat
     8881 ccatcgtttg ttcctccttt caatgcttaa acctgtttct tagttttgtc agcttcagaa
     8941 gtttaatgaa gagcacaata atagtaacag caagaataaa ccagatcaca atactcacct
     9001 gataccatat cttaaagatt accattcttt gttcaaagaa attccaaccc aaatagctac
     9061 ataagattgt aaatacaggg tagaaagcaa aaagaattgc tcgtttcaaa tcttcgattt
     9121 ctctcctcac tcacaacacc tcccaaccag cactaatcac ttccggaatt accaatacct
     9181 gcccaacgat tccttctaaa atattgtttt tggattttcc gacagaacag tattctgcga
     9241 caatctcaac cttgtcacca accacatcac cgctttccag attgttcaaa aagagcgtct
     9301 tacaggaatt gctgttaatc agcaataagc gtatatcctg ctctgccttt tcctgacatg
     9361 taaccgaaat acgatactgc ttttcagatt catcactgga agtaattggg tgaaactttc
     9421 ttgcaaaggg acgtagaatc aggttggaac caattaaaat agtagcggcg gtgatcgcca
     9481 ttgcatacat tcccgtactt gcaagaattc caatcgctgc agtacaccat aaggtggcag
     9541 cggtattcat tccacgtact gtccctctgt ccttgaaaat aacaccactg cataaaaaac
     9601 caacaccgga tataatactg cttccgacac ggaatacctg atccgaaccg tagagcatag
     9661 gaaacaaggt aaagaaactt gtacccatgc agattaacac attgatacga atacctgcag
     9721 gatgaccagt caattgccgt tctaatccaa taagaaatcc aagtgttaag gagagggcaa
     9781 ttctcaaaat aaaatctgtg taaaacatat gaaacacctc cctttaacta ctaacagtat
     9841 ataaaaaatc caaatacaat cctatggcta aagtcttgcg attttcttgc gatttggtaa
     9901 gaaaatagac ggactgaaac cgaaaatggt cttagtccgt ctttgtcata tctgatcatt
     9961 aaatttgtat ccaatacccc agatggtcaa aatatatgtg ggacaatccg gagcaggttc
    10021 aatctttttt cgcaacttcc ggataaaagc cataatgttg ctgttatcca gaatataatc
    10081 ttcctcccat acggcacgat aaatctgttc ctttgtaaaa acttcacctt tgttctggac
    10141 aagaaagtac aaaatatcaa attcctttgg agtcaggttg acttcagaac cttttatcag
    10201 tacttttctg cctcttggat aaatttccag atcccctgcc aggattctat ctgacagagt
    10261 acccactggc caatcgcttt cttctgatgc tcctaacaat aacgaagata cctctccacg
    10321 aatcatctcc tgaataccag aaagtaaatc cttcgcacaa tcactgtccg gtggatctct
    10381 tagtagtttt tctaatagcc agatttcaag agccgaatcc atcggaatat aactaatatt
    10441 ctttttcatc atgaatgcct cctcttaaat caactcttga tattttttct tataaaaata
    10501 tttggctact gaattcagca gcatatagag agataccacc agcagtaaaa atacgaaata
    10561 ccaaagaggt aatttcgtca gtccaaacaa cgacgcaccg ccgacaaatg taagggaagt
    10621 aaaagcaata atccccacta gagttataga aatgaccggt gcagatgccc tgctctggaa
    10681 aaaaggaatc ttggaagttc gcagaaaatg taaaatcaaa acctgtgtcc acatagattc
    10741 caaaaaccat cccgtctgaa acagtgctac atactgaagt tgcagggaag gatctgtaag
    10801 ctgcagatag gttgcccctc cacatagcat cggacatagg aaataataca aaaataggaa
    10861 ggtgacaata tcaaacagag aactgatcgg tccgaaagac agcataaact gtctcagtgt
    10921 tttgccggac cagtctcttg gggacagcat ctcttcctca tctacattgt cccaaggaag
    10981 cacaatacac aacgtatcat acagcagatt caaaagcaga atctgaattg aagtcatagg
    11041 caggaacggt aaaaaggcac tggcacaaat gatggaaaaa atatttccaa agttagagct
    11101 agcggtgatt ttaatatatt tcagcatatt ggtaaaagtt tttcgcccct ccattacacc
    11161 ttgctccaaa acattcaagt ccttttgcag caaaaccaca tcggcagcat cttttgctgc
    11221 gtccactgcc gtatctacag agattcccac atttgcttca ttcagtgccg gaatgtcgtt
    11281 gactccatca ccaagaaatc ccacggaatg accattatcc tgcatagcag agacaagacg
    11341 caccttatgt cccggagtaa gctctgcaaa aacatgagta tgttccacag cctttttaag
    11401 ttcacagtct gtcatttcat ccagttgagt accagttaag atacgctctg tagaaatacc
    11461 cacccggcgg caaacagaca cagcaatcgc tgcctggtcc cctgtcagaa tcttagggat
    11521 cacttttaat cttttgagtg cagttacaga ctcctttgct gtctgctttg gtgcatcaaa
    11581 gaaggataga tagccaacta gagtcatgcc ccattcgtca tcaggagtta ttttcttttg
    11641 cgctcccaca ttttttcgtg ccaccgcaat gaccttcatt ccgtcctgta acatttcacc
    11701 tactacagaa gatacactat gtctcgcatc ctgccccatg gggagaagtt ttccacgata
    11761 ttctacatgg ttgcagcgtg agaatatatg ttcaatatca cctttcataa taagctgact
    11821 attcccattg ctatcctgta caagtgtact gacaaactta cgagcatagt caaagggaat
    11881 ttcatcagct ttttggaatt ttgtcagaag atttgcgtaa tgggcttccc gccctggcat
    11941 ggtattgcag gcaagaatag cattatcaat ggtattgcag acaccagaat gatagcagct
    12001 attcagataa gcaagatcaa gaacctctgc attttcattt ccaaaaatat ccatataata
    12061 ttccagcagg atgctttcat tggtaagtgt tcctgtttta tccatacaaa gcacatccat
    12121 gctaccaaag ctctgcatgg cattaatatc cttgataatg gtctgctttt tgctcatagc
    12181 aagacttcct ttggcaaggc aggctgtaat aaccatagga agcatttccg gcatcagacc
    12241 tacagcaact gacagggcga aagcaaagga ttccatccat tttccatcag taatccccag
    12301 taatataaat acaatgggaa ctaatatcgc cataaaacgt atcatcaccc aggcgatgga
    12361 atcggctcct tttttaaagg aatttttatc atcagaatcc ggtttggtaa atcctccata
    12421 cagggtatct ttgcctactg ccagtacaat gccttcccct tttccactga tgactgtggt
    12481 tgccataaaa gcgaggtttt caagctgagt cagtgtatcc tgctctccgt aactgagttt
    12541 tcggcagctc ttttcaatga tggcgctctc accagtaatc gcagcctgag agataaagag
    12601 atccgtaatc ttcgtcaatc tgatatccgc cggaacacga tcacctgcag agaatagtac
    12661 aatatcccct accactaatt cttcgccagg aatctctatt aactttccat cacgcctcac
    12721 tgtgacgctt tcatgaacta ggcgatcaag ctgagcagca gcgctctttg ctcgcagctc
    12781 ctgaatcaga cgaattacgc cactaattac gatcatggaa aagatgataa tggcggtagt
    12841 agcatttctc gcaaaattgg atgctacgaa aacatccgta acaagagaaa caatgcccag
    12901 aacaaaaaga atcacatgaa acgggttaat aaatgcccgt ctcaaccgtt gcatcgtcgt
    12961 atcatttttc cgcccattga agctgttcgc tccgtatttc tcccgcattg aaccgatttg
    13021 ctcaatggat aaaccatccg gagatgagcc aatgtctcga taaatttctg atgttctaca
    13081 gtatgcgtat tttttgatac gactatcaaa tagtgttgct tttgacacga aacatcccct
    13141 cctttataag tataagtaat aacattttaa cacagtgctc tgaaaattct atatttatat
    13201 tcttgctttt ttcttgcaat ttactttgat atggcatttt tcagctcgat caaatccgtc
    13261 tcgttagggt gagataaatc cttatcaaag ttttcaacca ttccttctaa atttggagca
    13321 cggatggata cggcaagcat tttctcatag cgctctcgca cataaatagg catttcccct
    13381 tgacacatat gagtgctgac aaacttcttc ttggttttca ccttgaccat gtatcaaatt
    13441 tccaaataac tacttagaaa agttgctcat aataatattt gtattatgag tgatagaatg
    13501 acaaaagtgg cagtatggaa aacgagtgta aaaacaatac aattagtttg tatggaatat
    13561 ctgaactaca taattgacca ctctgtaatt tatgaaaaaa tacaatgcgc aaattggaac
    13621 gggacagaag aaaccaatag tatttttaat aattcccctt caccattgat tactcttatc
    13681 tattcttgcg atttacaaca aaataaaaca acataaattt taaatacgtt gactggcata
    13741 attcatatgt atagtaagtc tacaattaag atgcgctttt tctaatccaa atatattaag
    13801 aaaaaaattt tgcgaagcca ttaatacttt gtttaagatg tctttcataa atataaagaa
    13861 taatgctctt aacaaaagcg tgcaaattcc taaagaaatg cacgctttca ataattatcc
    13921 tataatcttc cagttgctat ccttatgtag cactaattca aactgcgata cctgtgttgc
    13981 ctttgtctga ttatcaatga atttcacggc aaccttgact ttcacattgt ccccatcaac
    14041 cgtaaagata gggtttacca attcagaata gaggtagtcc ctgccgatag actcaagcac
    14101 atttccagac acatagtaag caagctcctt ttctgtggct gttgggtata gcttaaagaa
    14161 agtttccaga aatgcagtag cgtcatttat ggtatcagcg tctacacttg cgtctgcttc
    14221 tggtgtcttt ggttcatagt ctgatttctc gattgctggt gcaagggtag ggttctgaat
    14281 aattaccata ttcccgtcat cgtccacatg aacttttacg gtataggttg ccttaaccgc
    14341 tgttgtctgt tccccctctt ctatctgctg atctacttcg taggtagcag aaaaagtgtc
    14401 cgttccagac tgttcaatgc tccatacaat cacatctgta attgtggagc tggtcggaat
    14461 atcggttcta atggtatcca cattcaagtc ctgcaattcc tttgtcagat aaccgttgat
    14521 tgcctgcgtc cttgcttcga tagcttcttt gctgttactc catgtataat aagactttgc
    14581 aaagttcttc acgaaatttt ctatcccgtt ggtgtcctgc aagcgaagct ctatcacttc
    14641 tttttcatgg gtcgtatgct ggtcgatagc cgtaaaattc ttatacaccc caaagctcac
    14701 gctttcgata agcactaccc acaacgcaat cacggttttc ttatgtgtgc ctaccttgac
    14761 agtacgcacc tttttttctt tgacattttc tgtcgatttc ttattcttct taaacatatt
    14821 ttcaagtcct ttctattctt ttactcgtcc tgcaccgatt aagtgctgtt gccagtaact
    14881 actacttagg tctgcatatc ctatcgggtc gcctgcatgg tacattttgt tatttcctac
    14941 ataaatgccg acgtgtgtta cataacttcc agcgttatag gtggaatgga aaaacaccaa
    15001 gtccccagcc tttgcctgcg aaagaggaag atgttgggtt gcgtcatact gggcttgtgc
    15061 cgttctcggt aaggagatac cagcttttcc atagcaccat tgcacaagtc cgctacaatc
    15121 aaaggaagtg ttcgggttac taccaccata cacatacttc cagccttgat atttcaacgc
    15181 ttcattcata atcgcttgtg ccgtcgcatt atcaaagtgt gccacggtca gatattgatt
    15241 gactaattcc acatagaaca tatttccata gccataccgc cagcccccgt tcttcgcaac
    15301 agctatcggg ttggtgtagg ttactttctt tccaaccgat ttctcacgtg cgaagctctc
    15361 tgcaaggtta aaggtgtgtt tctttccttt gcctgccaca tatcctacat agccaccgcc
    15421 atagttatag gattgtatcg ctacattcaa atcgtcgata ccttgatttt tgcaggaaga
    15481 aagcagggac gcaaaatact tacacccctg cttgattgag ctttccgtat ctaaagagtt
    15541 gggcagtagt cccagacttt ccgaactctg cataacatct tctgccgtac cgccactttc
    15601 tacttgaatg atagccagca acacatggac gtactcggaa atgccatttt ctctggcgta
    15661 cttttctacc ataggctgat gtttcaagac ttctgcggat aagttcatac ccgtaatgcc
    15721 agaagaaaaa ttgctgttct cgtcgtcgct gtccgcacta atcaagacac ccaaaaaaag
    15781 caccagagaa aagaggatag gaaacagact gccaatgata gcgatatgtt tcagtttcag
    15841 tttcattttt tcttctgtcc tttcttcgtt acaatgttac ggtttttctc tgtggtctgc
    15901 tggttctttt ggacggtttg ggtctgctga acctttgtcc tgcggtcttt ggtgtaattc
    15961 tggcgtgttt cctgcgatac cactttttct acattctgcc tatgtactgg ctgtgccgac
    16021 tgggtcgctt tcatatcaga agcaccagaa gataacggac gctctttgat aacatttgtc
    16081 ttgactggct cacttgcttt agaagtcgga gctgtggcag ggcgtttgat ttcctgcatt
    16141 ttttcaacac tcgtctttgg aacggttgaa gctgtggctg gtcgctcatg gggacgggta
    16201 gctcccgttg tcgctgatcc gtccgttttt ctatgccttg cttcttgtgc cttttgaagc
    16261 tccatacgct tgacagcgat acttttctta tgctgttcct gcttttccaa gcgtcccgtc
    16321 tgtctggact gctgttcctg caccatacca cgcttgaaat cagacacgct ggactttgcc
    16381 ttttcctttg cggaatacac cgcataagcg gtctgtgtgg gcatatcctt gatattctct
    16441 ttgacagcgt ttgccttgtc tttcacttta tttttcgtat ctaagacagc acccaccttt
    16501 gagcctgcac gctgtcccat actggaagtg gtattgcccc gattttcttt agaagccgta
    16561 ttttttcttt cggatctctt gccagcaacg gcacttccag ctaccgcacc agccacaccg
    16621 cccgaaatac cgccagcact tacagccctt gcaatacggt gttccatacg cctagcccga
    16681 tgtcgcatga acagatacgg tctgcggaat atcctgcgtc ccatgctttg actgtcgcca
    16741 gcgttgagag aaaacatact cattaagtcg cccagcttca tatagatacc agcgaaacat
    16801 actatctgca agaacgccac catgaaaaac ggatagtccg tggatatgtt ataaaacata
    16861 ctggaaatgc taaacgcaac cgttataatg agcgttattc ctgcccgtgt cattatggta
    16921 ttaaataccc tcacgattgc ctgctttgcc atgctttcat agctcggtat catggaaagt
    16981 agaaagctga taggtaaaaa catagcgaag ataataaaaa gtatctggct gaacaacatc
    17041 atacccgtaa gcaagaatac aaatatcgta ataccaagat taaagaacaa gaggaagaac
    17101 accataccta aacggtttac cacctgcggt atcgtcagat tgttgttgtc gttgtcctcg
    17161 atttctgttt tcacgacttc ttctctggtc tttccgtcct cgtcctctgg acttgccgat
    17221 acgagagctt cgacacggtc tgtcccgatt tcctctgcgt tgctgtttcc aaactgtaag
    17281 agtagccacg gctgttgtac ttgaatagaa aataggctgt cccgtatcaa gtctacgctg
    17341 tccttgccct cgctgtcaga gttggggagc atgatttttg ttcccaaatc aagcgaagcg
    17401 gtactgatgt ctgatgaaaa ttcatttatc ttcttgatgt agtcgggagc gtaggcaata
    17461 aacgaagcgg acaacacgaa caccaccaca aagttgataa cagcgtgaag tgccttgctg
    17521 gtttcccgtt ttatcagtcc cgtataggca acatacattc ccaccactaa gataataaga
    17581 agcaggaaac caacatagaa gcccgtagaa gaaaagccgt tctcggtaac gcctgcaagg
    17641 gtctgtatgc tttttccgat actgtctgcc atatcgttaa taaaatcaag tttataggct
    17701 tcctgcacca catagccagt tgcattgctt aaataaaggc ttatcgtcca gacaaagttg
    17761 gtaatgcagt aaagcccgta ctgtaccgat tttccgatac cgtccagcca gttccacgga
    17821 agccacgacc agctattatc gacgaaaaaa tcaagctggt agttggaaag cggatatttt
    17881 gaatatagat tttctgcgtt tatggtatcg tccacaagcc ccgtcgcatg agccaccgtc
    17941 cccagaagtg aaagcaggat aagggaaagt gccaccacga acagagccat tttgagaaag
    18001 tgaagtatct tcctttttgt gaacgcactc tgtatccttt ctttcatcac tccacctcat
    18061 ttctctgtac gggcggtcta gtatcaaagg cgtgtagtaa ttcttcaaag actgggtgta
    18121 tctgcaccac accgacacgc ccgtataagt cctgcaataa acattgtccg ttctccaaat
    18181 cacgcaagcg tttctggttg ttttcgtcgt ccttgtcgat accgaaaaat tctaaggtct
    18241 gctttatctc gttgatgtca gtagaacgaa atgcaaactt caaaccgata ttgtttttca
    18301 gactttcctt tgacacgtca taggcagact gggtaacgaa ataaacgcct gcctgcatag
    18361 ctcgtccagc acgaaccagc ttatttgata gggtttcgcc ttgtgccaca tttaagaacg
    18421 cccacgcttc gtccaaatct acaatcttaa aaatgcttct gtccgaatgg ataaaatcaa
    18481 gggcaaaggt actaatcaca atcagcatag acaccgacaa taattcaatg gtcgtgtatt
    18541 cctcaaaagt ggtatcttta tctggcagta caaggtctgc tacttgaatg atattgagct
    18601 ggttatcaag gcttatagca ttttctaccg tcccgtctga aaacagcagg tgtgcaaagt
    18661 cgtagtccgt gaagctgtcg atatggtctg cgatattgcg tgagatagcg gtatcttctt
    18721 ttctcaattc ctcaataacg tgtaacagtc ctctattttc actctgggta acagaacgca
    18781 ccgcttttcg taacacgggg aatttttcgc cgtccctaga ggaaataccc gtaaggaatg
    18841 ttaagatgtc gattgccaga ctttcagcgt ctttcacatt cttcataatc acaaatgggt
    18901 caagaagtcc tgcattgtcc ttgtcgctgg taaggtttac gatattgatt tcatgggcta
    18961 tctctgggag cgtttctttc cagttgccac gctcactttt agggtctaac agaagtgctt
    19021 gaccgccaaa cagcaccgca taatatacaa gtagattgtt gcagaatgac tttccaccgc
    19081 caagacttcc cacaaaagca gaagcaaggg cattagtaac tgtgcctttt actccctgcg
    19141 aagctaaaga cggttgcagg tacacatttc ttcccgtatc aacggaatag cccatataga
    19201 tacccgtgtt ttcccctaac tgctgggtcg caccaaaacc aagcccagcc aagaagtctg
    19261 actttacata ctgcacatag tcattgatat atcgcttgct ggcaggtaaa aattcagaat
    19321 gaagtcccaa catatcgcca gcaggacgca ccagttttac attgaggtcg tcgtaaaagt
    19381 ccttgacttc atcacaacgg cgtttcagct cgtcaagatc gggtgcagac acccgtatga
    19441 cgtagcttaa cttatacata ctttctttgc tctggtctaa atcggtttcc agctcgtcca
    19501 cgctgtctaa agcgtccacc acatttgagc tggtttcact tcctgcttga taggcgtgat
    19561 tatccaaatc tttcaattcc tttttcttgt tgcggactgt cgttaatgcc ttacggttct
    19621 ccacgatttc tacattcata gaagtatcaa cggggaatgt gaattgctgt tgctggaaat
    19681 agaagatttc agaggacgga aaatccaact ccccgacaat tacattgacg gtaaaatagg
    19741 acacatagct ttccttgtcc tcatgttcca atcgtaaata ccgctggctt tcctcaatca
    19801 cacaccttgt cggacggata aggtcgtagt attttatcag cgtttctttc ttcaatttct
    19861 tctttggtag ctggtactca taatcttcat aggcgatacc gtccctgccg taaagatgtt
    19921 ccatgagata cccaaaatca tggatttcca agcgacgcac cttaaagcga cgggagattt
    19981 tattttccag taacttttcc attttcatgt aacggttgat ttcatcattc ggcatggaaa
    20041 caaaatcatt catcagcgtg tggttcactt catgaagaaa ttccttgaac gtcagccatg
    20101 ccgatttttt gatgttcttc agattgagct gttcttccgt aaccatgagc ttaaagccaa
    20161 gaaaaaagcg gtagtccact tgattgtccc caatcattga tactaacgct tcggtctgtt
    20221 cgtctatttt ttggactgcc acctcacgaa gtttgcctgt taccagtttc tttgactgct
    20281 cctgcatact tctaatggag ctttctgtgg caatctgcaa cgcatgaatt tttccctcac
    20341 gggactgtgc gattagctgt cgaaaactgt catgcacgat aaatttctgc tctgcggata
    20401 gaaaagaata gttgtacggt atcagctcat agtaggcgaa cacctcattg tccttgttcc
    20461 agacaaggtt attgtcaata tattttatcg ggaacatagt tcacactcct tactgccgtg
    20521 attgtttcat tcaatatctg cttatgcagt tttacggctt ttcctgcata agtgatttta
    20581 ggtcgcaagg cataggttat ctgtgatttc aaaaagctgt acggcttctt tccgtcaaag
    20641 gttttctgcg acataaacca agtgagagca acgggaatac cgaagtattt gagaaatgct
    20701 ccctcaatca tggaaagtgg gggcatatcc ccaaacagaa tgatgataaa ttccgtaatc
    20761 acaaaccatg taatctgtgt aaaggtaacg ggaaagggta agttaaagtc attgattgca
    20821 tataagactt tttccacgtt ccagataccc gtgtagcttt taatcttttt caagttcttt
    20881 cagccccttt cgtaagttct tcaaagagaa aaggacagcc attttcagac tgtcccttaa
    20941 agagaaatac gctgtcagta gcaacaatgc ttgttgctga tcttccagcg ttaatatggt
    21001 atctcacaga tacctcggct tgtttcaatg aatcgaccac ccatatccaa atctcgcccg
    21061 taggcttcat agtcaatata gttctgtaag ttagctggaa tgtccccaag tgcgttcaat
    21121 tcgtctatgt agtagtaggc aacatctgtc atggtttcac aatcgggata aaaataaata
    21181 tcgtccttat gttccacgac ttcttccagc gtcccatagt ggctgataaa ttcgtccaga
    21241 cactctacga tatagtcggg aagttcctct atcatttcat acatttcatt gagttcttca
    21301 atggaaacat attcccctat gtcgatagga aagttgtcgg tatcatggac agcgtattct
    21361 tcgtactgtt cattcaagcc gattttttct tttacatctt cctcgtcaat agggaaagtg
    21421 aaccacgcac cgactaaata tccctcattg tatctgccaa gattagcaat ataaaccgcc
    21481 atatcatcaa tcataagcac cacctcattt cctactctgg cagttcaaag ataccgtgtt
    21541 ccgttactaa aaattttccg tgttcctgca agtgagaagc ataggcttca aagtcaaaat
    21601 agtattcttg acagtcctcg gataaatgtt tgaatgtcgg gtcgttcatc agcttttgcc
    21661 ttgcaacatc aatcatgctt ttgcagtcgg gataaacggt aatcacgttc ctgcaaatat
    21721 aaagtgcttc taaattctca tacacggtaa gtagtgatac atattcttcc tgcatatcac
    21781 ttgaaagctg gcggtacata aaatctaatt cgttgagctg atacacgctg gtgtgttcgt
    21841 ggacttcatc agcataaggc agtacctttt cgataatgcg gtaatcccca ctttctgcac
    21901 cgacacctaa tttttcttca aactcggcaa catctatcgg caggtcaaac cagtatgata
    21961 ttgtttcttc gccagttgtt cctctcgttt ccaccagcac ccttgtttcc tgcattgttc
    22021 ctcacgtcct ttctgttgtt tcctcacatc ttttatggtc tggtacgaca taccaaacac
    22081 atactttgaa aaatcttcta actgtttttc ttcataatcg gcagggttca gtcgtttcat
    22141 ttcatgccac actttacgct tgtaagaggg caggtcggaa atgtattcac agccgacttt
    22201 tgcctgcata tccttaaaaa tatcgttcat ttcatcactc caatctgaaa ttttggcaag
    22261 aaaaaagcag tcaatcctaa gactaactgc tttgtgtata tctggtatta gattatgaaa
    22321 ttgagtttat caattcttgt acttcattaa aaaatctgga taaatgaaaa ccatctgcaa
    22381 cggcatgatg tatattcatt gtaagaggca ttatatatct ttctcgctct aattcgtatt
    22441 taccccaagt aataactggt gcaagaaatt tcccttcgtc aaaaacatga acatcaaaat
    22501 gattgtattt tacccatggc aaacaagata catcaaaaaa gttcaaaggt tgatttgcta
    22561 cgaatgcacg ttccatttta tgtttctctc tatctaacat aaatcgttca tgaaattcaa
    22621 atatgctatc agaaaaatct gtaaccattt ttgtgaaatt ctcatcttct ttatgaaatt
    22681 cagcatagga cggggaaata tagtcccatt taattaaatt acctttattg tcccaactat
    22741 atttaaactc atcatgttca ttaacgacct ttgaaacaac ccatatcata gctggataaa
    22801 atttcaaatc atggcttttt gaaaattcaa ttagatttgt tacatcaata tctacggtta
    22861 agctcataac gatacgcata tcatcaatgt aactttgaaa caatttacct ctattccatt
    22921 tttctaaatc aatttttgta tatttatatt ccatgtccat tctcctttac attttgaaat
    22981 tcagtaaaat gcaaggctct ctggataaca tttcactcca tgcaagtcta ttccattaag
    23041 ccttgtatgg agataataca atacacaatt tcataatatc cctccttatc caataaacct
    23101 tagtttattg ttttcatact cagattatac cattcatata taggctctac aataaaaatc
    23161 attatgcacc aataatctta ttgaacagct ctaacagtac atctttcaca ccgccagcgt
    23221 tgaatactaa gccgacagca ataagggcaa ctaccaagaa gccaataagt tttgaaaact
    23281 cacgcttgaa ccctaagtag ataccgataa ccacaatcgc cataagcact aagctctgtg
    23341 catttgataa aaaccattga tacaaatttt gtccgaaatt catttaattg tcctcgcttt
    23401 cttctaattg tttcatttca tagtcagctt ctttgccgat actcaaaata cacatcaaga
    23461 ctacgccgat acccattccc atagatacca gcaggaagtc ttttactaat tcccacattt
    23521 tttatcactc ctaactttcc accaaatctt ttgcaggggt cgtctgctgt ttgattatca
    23581 tttcatgctt ttcactcaaa gttgcctgct tttcaatcgt ttccatatag tcagtcccgt
    23641 ttcccttatc aatctttttc agcattttca aggtaggtgc tacctgcctt tgtacccacc
    23701 gcaatgtacg gtctaaggtg taaggctctg gctttgtcgt cagcttcaag ctctgtctgt
    23761 tatcgccgat aaaccaagcc cagcggtcat tgagtttcca gtcatttttt cgcttgtctg
    23821 gttcttcatc aacaaaccgc acatactggt tgatgataga aaaggcggtc tgttctgcgt
    23881 cataataggt cagcaaatct cgtactgcat aataggcacg ctcatttcta agccgtatct
    23941 caaaacggtt gataatgtcg gcttcattaa gcggtgtacc caacttgacg tactgctcat
    24001 agtccttttc atagatacag aaatatacat ctgatttcag cgaaccaaga taaagagtgc
    24061 gtcccatata ttctctgtcg tcctctctgt gcttgataag ctcgcccgac tggtaaaact
    24121 tataacttct ggactttccg atatattccc gtttcctgca tttttccgca agctctggaa
    24181 tatccaaaat gcccgtatgg tcgttgatag caaggtcgat acgcttcatc acgccaccgt
    24241 ctacgagtgc gtccatgagg aagtcatacc agcttctttg ctgtgccaac aggtaacttt
    24301 caaactgcct gcaaccacgc ccctttaact ctaaaaggac acctttttct tcgtcagccg
    24361 acgtatagat aaagatgtcc cctaaagagt aatgctccgt atagctgtaa tgtccgtaat
    24421 cttcatggag catatagttg atattcagtt ttaatatatc tttgatgatg tgctgtatat
    24481 ccagcgtggg aaaacgaatt tttacataat caaacaacat ggtaagcggt gcttctggat
    24541 tgaaccttgc cagttcctta tgcagagttt ccagaagttc ctttgacggc ttcatttttc
    24601 cgctttctat cttattgaga tattcccttg ttataccaga agccaccgcc agcctgcctt
    24661 gtgagatacc gtaagcaatc cgtttctccc gtaattcttt tatccattgt tcttcattca
    24721 gaaccatacc cccaatctgt aaagtgtgaa gtttttaggg gcgacttcac aaccgatttt
    24781 tgctaggaaa ccatgccaga agccttatgt ttcctatgtt ttttgtctat tgtctatttg
    24841 caaacgtacc cctctgttag ataccgaggg gttttacctg ctggcgtggc ttacgccaca
    24901 ccagcaacgg ctagtccgtg ccgtcgcctt tcgcttcgca cgtcgccgtc ccgtcctgcc
    24961 ttgcttgtgc aagagaaccg atagtcttca aaaaatcatg tcctttcggg actaagggcg
    25021 tgtaaaattc gcttatcacg cttgttccta catcacaata gccacgaccc ttgatacgct
    25081 tctggaaaaa ctgctttttc acatcagaac cgaacagcat accatatcct aactcgctga
    25141 tacgccccaa tcctacacgg aaattgaagt tatctctgat accgtccgag aaatactttg
    25201 cgtctggacg ctggcaggca acgataagga aataacctgc ttgtcgcccc agcataacga
    25261 ttttctttaa ctggctaagt aggctcacgc tttctttcgt tcccagcatt tcaaaaaaag
    25321 ccacatattc atcaaagata agaaagcagg gtggcagtcc cagataggcg tagttttcgc
    25381 ctgtcttgta gttcgagtgt tgcttcattt cctcacttcg ttgtaccatg ccctcataaa
    25441 aggaattgac gcaatctatc atttcttctt tggtgtggta cacgtttccc atgactgttc
    25501 ccaaatctgc aaggtcagcg ttctttgggt ctaagatgta aaggacagcg ttggtatgca
    25561 gtaaggcttc aatgagcgtc agcagaaagt aagtcttacc gccaccagtc ccaccagcaa
    25621 tcagggcgtg agggagtgcg tcatattccc agacaagatt tttcatcagt ctaagacagc
    25681 cgttttctgc ccgtacttca tcaatggtaa tgcggtttgc tatcatatca taaagcaggg
    25741 tatattcaat ataaccgtca tgcagtgttt tgtcggttag ctcacaatac aagccacttt
    25801 ccaatttatc ctctaaccgt aaaagctggt cttgatattt ccccagcgta atttcacagc
    25861 ggatatgaag cagtcccttt tccatttgat aataaatctt tggaaaccag acgatttttt
    25921 cccttgatct gctttgcagg tcagtaaaaa aaccgctgtc ttgtacggta tcggcttcat
    25981 accacttatt ttccagtatc attcttgcca gcttttgacg gtgcaggagc ttcttgaaac
    26041 tgtcgtaaca gaagcgataa tacagaaaag cgaccaatgc acaaacacca gtcgctatca
    26101 gtatggttat caagttgtaa ggggataacg tcaagccttt ttccaacaag ctgaaatgtt
    26161 cccaatcggt acgcatgagc tgtttgatat tcagtagcag gagaaccgcc acgaatacaa
    26221 acagaagcgt ccctatggaa aagtgataga caaggtgctt gtcgctggct ctgatacggt
    26281 gtcctttgtt ccaaatctta cgcataaatc aatcactcct ttctgggtac gaaaaaagca
    26341 gtcaatccta agactaactg ctagttacga tttatttgaa atgtgaaatc tttccgtatt
    26401 tttcgtatga atatttaagc atttgataaa tatcttctct aagaatatac tggtatgttt
    26461 ctgagattgt ttcatacacc ggaataggaa ttgcttttga tgaagtcctt tggttcgatt
    26521 ttatcaacac tctttttgct ggcttagata ctgttttatg aacccaatcc gaattcaata
    26581 aacagtgtcc cataggtgta ccagaactgc aatatggttg attttcattc ttaataaatc
    26641 taagcaataa tttataatca cttgaagata agttatccac atctgtccaa agaaattcta
    26701 aactctgttg ccattcttct gcttgtctct gtttgttacg ttgttccaca ccttttgaat
    26761 aattatgata ctgtattttc tgtaccaacc atattactaa ttcgatgatt agaaaccata
    26821 cgcagaatag gaaaacagca tacccccaaa tagtgaattt taccaataac ttattatccg
    26881 taggtgtaac gtaataagct ataaaagaaa aaactattgc aataactgtc ggtactaaat
    26941 acttttcaag aaatatcttt aataaatcta ataacttttc actcatgcat ctatgcccct
    27001 taattaaaaa atcacattaa aaatattata cgatttctta attgcagagg caatcaaaat
    27061 atatttgatg aactaattat tttttcgttt gtccctgcgg tgtatggttc tggggattgc
    27121 ctgcgttttg attgccttta ttcttcaaca caatatcctc tgcctttaca taccagtcca
    27181 catctgcacc cgtataggtc tttcttgata ctgtatcggc tacgggattg acaagctcca
    27241 caactgcatt gtacggaaat tcccttaacg gtacttctgg cggtacggac acggggataa
    27301 ttccaccatg cagactgcac ttcaaatcat agatacgctt tttaagctga gtactcggtg
    27361 tcccgtcctc attctgcaag aatacatcac gcaccgccgt aaattttaat tctccaaatg
    27421 ttttctcttt gtcaataaca aacccgtttg ataatctcat aaattcttca ctcctttact
    27481 tttcttcaac tgctacaata tcatcagcaa ctaacacata atcggtatgt cccatatccc
    27541 cgattgcgta acctctgccg tataacttcg gattgacaag ttttactttc tgctcatact
    27601 tgaaatgctt ttcaccagcc tgcacgggaa tttctaccac aacattttca cctttctgta
    27661 cgtcagaata aaggttatag cttcgtctgg ctaaaaccct gcggttattt ttatctcttt
    27721 caaagactgg ctcactttcg cctgcaaatt caagagttcc aaaagactgt gtcatatctg
    27781 gcacgacata tttcatttcc atagtgtttt acctcgtttc tttctatttt tgataagttg
    27841 attgattgtg atttcttatt ctggcggttg ggtaagtacc agcaatcccc cagatagtaa
    27901 agggtaaaat caatgcttac gcacccttga ctatccgtgt tcttgcaggt tgtagacaga
    27961 caagccagaa taagcggaag tctaccgttt gaccgcttcg gcggagaacg tgatttttct
    28021 ttcatcatac cgttaccgcc ttatgatttc ttcattttac ggcgtttgta aagatatgtt
    28081 ccagccagtc caccagcaga cgcaaacaac atcacgataa atgccattac atttgtactg
    28141 tcgcccgttt tgggactgtc gctaggtctg tttggcttct ctggtgtctg tggtgtttcg
    28201 ggtttctctg gttcttctgg cttttcctta aaggtaatcg tctgtccctc gtcctcaatg
    28261 tctttatgct cggtaacttt cttcggcttg tctggattgc ttaaatcgta taattcctca
    28321 aaagttacaa gctgtttgcc gtcaagggaa gtagcgtcaa aggtaaaggc aacttccact
    28381 ttcatagttt cgctgtcagc ggtaaaggta taatcacttt ccacacgttt tccattgata
    28441 agaagctctg cattttctgc tttcaacatc tgccagccta caagtttata ctttgtaccg
    28501 acttctaagc cctttaaggt tacggtatca atgattgtaa cgtcttttcc tgcttcaagc
    28561 tctttgttgc cgtccttgtc cgtagcagtt gtatgaattt tgatgatacg ctctgtgatt
    28621 aaaacagttt gcccgtcgtc ctcaatgtcc ttatgttccg caactttcac gggttcgtct
    28681 ggattgctca aatcatacaa ttcttcaaag gtaacagggt tcttgccacc taaagcagac
    28741 gcattgaatg tataggaaat ttccactttc atttcttcct catcagcgac aaaggtataa
    28801 tcattttcta cacgtttccc gtcaatgata agctcggcgt tttcttcctt taacatctgc
    28861 cagcctttga gctgatattt tgtgcctttt gtaagtccgt ctaatttgac agtatcaaca
    28921 atcgtaacct cttttcctgc aaggatagtc ttttcgccgt ccttgctggt tgctgtggta
    28981 tggatagaga tttctttttc gtattcatca gtcaaagtcc ctaaatcaat cacaagatta
    29041 tttcttgata ccacgatttc aaaaggtggg ataagctcaa agcctttgtt actatcacaa
    29101 cacatttctt caattatgta ggtatcataa ggtaacgcac ccttgctgtc gtctggttca
    29161 gaagtcccaa accatacacc gtcctcgctg gtctttcctg cgttggtatt gtgcttgtga
    29221 gaagcccagt cagcagaagt ggagaattgc ccgttatcgt cagttactac gacatgactt
    29281 tctcccgtcg tcttgcttgt gatccgaaac ggaacatcag caagacgctt gtgtgtgcct
    29341 gcaccgattt ttacgccctc aatatctcca cgcttaatct gattgtagat agaatgggct
    29401 tcgtcggtta agtccacgat ttttccattt tctgtgattg caaaatcaat cggttttgca
    29461 ccgtctgtca aatatccgtc gggtgcttca ctctcaacga tacggaagtt tccataaggt
    29521 aaaaggtcag cagaagtaga agcgacaccc tcaatatctg tacgaatagt ttttaccact
    29581 tcatttttct tgtataactt gccttcaacc aatactgcat tatcatttaa ggaaatgatg
    29641 tcaaaggcag tatctttcaa ggtagcactt ccttgtggtt tcgtatcgcc tgtttctaaa
    29701 tctcgtttct ggattttcac accgccacgg ataaccttgt ctgatacgga aaactggtta
    29761 cttccagtca atacggcaag gtcgccgtcc tcagtaatct gtgttacata tacgcccttt
    29821 atctgttcgg atttatcgcc agcctgcata tatgcaccgt caagtaagta gccttttgga
    29881 gcttttgttt cctcaacggt tagtgttcct aaaggaagaa ccgccttgcc gtcctgcata
    29941 tagaaactgt cgccagatac cttgtatgcg ttcgctaatt ttgtgatgta gtgggttgtc
    30001 tcgtcgctgt cagtttcagc gattgtcttt gtaacccatg tacgagtagc ttcggcaggg
    30061 agattgtctt tgttatagaa gcctgcataa tacttccatg taaatttcgc acctgctaga
    30121 gaagcgttcc cttgcggatt gtctttctgt gtttccatat caatcttgaa aagctcaatc
    30181 aaagtatccg ttacctttgg tgtatcagat acttttaagg ttgctgtttc gccagctttg
    30241 attgtcaatg gatagactgt tttatccact ttatatcctg ctggtgcaga aagctctttg
    30301 atatatactg tccctgcttt gacttctaca acattcgtat ttccgttttc atcagtcgta
    30361 agggtggcaa gctgttttgt gcagtcctta tcagaaaaga caccataagt tgcaccagag
    30421 atagagtaat ttccgttacc gtctgtaatg ctggtattac tggaagtctt ttgcagcttc
    30481 gcatttccga cattcagctt cgcccagaat tgtcccaatt cctgcccctc gccagagtag
    30541 atataaccgc cacattcata gcgtccttta ttttctttga caaaggcttt tgcaccagcg
    30601 aaaacttcgt cctgcgtagc ttttggtatt tcatcataag aagctcgcac gttgtcgcat
    30661 tgccagccaa gatgtacgct taatctctgc cagacaacac attgttccaa cagataaact
    30721 tgtttgtaat tcaattcctt gtgagcttcg ccatactgtt tgacatactc taaggataac
    30781 gccacatctg aaatctggtc ggcactcatg cgtgagcttg cgtcagttct ggtcttgtaa
    30841 ccattcttga aatctgtatt gatgtcaata caataggcag tttcgccctc aactttcatc
    30901 atgccctcat tgaatgtaga accgatagaa ccgtcattca ttactttttc aacgataccg
    30961 acacgctctt ttgactccgt ccagtattgc ttgctttctg catgaacggg tgtagtcggt
    31021 aaagcagtaa cgatagttgc aagagctaag aagcccgtac acaatcgttt taacatcttt
    31081 ttcataaatc taatgctcct ttcattttgg gtataaaaat agacgctcat ttctgaacgt
    31141 cttacattta aaaagggata ctattttaac aagtacccct aaagtttagt atttaattgt
    31201 ttgtgttccc acactctata tcaactacta ccgaaacatt gatatagttt tttatctctt
    31261 tatttctgat tttatcgccc tcgctcgtaa agacgatatg cttgaaaaag tgcctaaaat
    31321 taaggatttc tacatagttc gtctttgtag caacacacaa gtctccacat gtgtagaatg
    31381 agggaacata tctacaggtt gtgcctcaac acatttaaat ccgtgaacat ttagatagtc
    31441 taaatccctt gctaaagtag aaggattaca agaaacatag acaattcttt ccggattcat
    31501 tgaaacaata gtctctaata ccttttcgtc acatcccttt ctaggtgggt ctacaactac
    31561 tacattggca gtcttaccct ctctatacat cttaggtaca atttcctctg cctttccaac
    31621 ataaaattca gcattatgta tattgtttaa tttagcattt attttagcat cttttatggc
    31681 atcatctata atttctattc cataaacctt tttagccttt tgtgccaaaa ataaagatat
    31741 tgtacctatc ccacagtaaa tatcaaaaac agtatcagta tcatttaaat ttgcatattc
    31801 aagagcctta tcatacaata ctttagtttg gacagggttt acttggaaaa atgataatgg
    31861 tgaaatctca aacactaagt cacctatata atcgtttatt tttccatctc catatataac
    31921 tatattttct cttcctagaa tagcattcgt atctttttga tttacattta caaccaaagt
    31981 cttaaatcca ggtatatttt ccttaagcac tgaagctaat tctttaagat atggaagttt
    32041 gtttccattt gctactaata caaccattac ttctttagtt gtaaaaccta tttttgttac
    32101 taaatgccta atcaaccccg tgtgagtctt ttcatcatat atacttacct tataggccct
    32161 tatatatgtt tttattattt taacaatttt atcatttatc tcatgttgta ttacgcattt
    32221 ttctgtcgat attacatcat gagtcttctt tttatagaag ccaataattg gaataccatt
    32281 attcttttgt attggaaatt gtgctttatt tctatatcta tatggattat ccattcctat
    32341 agtgtcatga acttctacat cctctaactt acctattctc gaaagtactt gtttaacttc
    32401 atttgttttc atttcaagtt gtttattata atctaagtct tgtatctgac accctccaca
    32461 atccctaaga ttctcactac acactctttt caccctgaat ggagactttt ctattatctc
    32521 aacaatatct cctacagcat aatttttctt tactttatta atcctaactt taactttatc
    32581 ctgtattaat ccgccctcta caaaaactgt aataccattg tactttccta ctcctactcc
    32641 gccttgacca atgtctacta tatctacaat atacatctcg tttttcgaaa gcataaatca
    32701 ccctccaatc aatctattat atttaaatct ttacaaactt ttttctttaa atttatatta
    32761 aaatattgct attaattaaa ctatattttt tactaagcta caatatttta ttttaccaaa
    32821 aaaaaaggag cttataaagc cccttagtaa ataaatttat tatttttctt ataaaactct
    32881 agttgaacct ctatatacca cacctcttga tgcatccaca gttacaactt ctccatcttt
    32941 tactagattt gttatatcag ttgcagacac aataactggt atatctaaat ttatacctac
    33001 aatagctgta tgtgatgtca ttccaccatt ctcagttact acagctcctg ctttttctat
    33061 ataattgttc atttcagcat cagtagcagt agaaacaagt atgtcgcctt cattaaagtc
    33121 tacacaagca tttccattct ttattatacg aactttacct tctaccgttt gagtacctac
    33181 acctatacct tgcacgattt cttcgcttat tacatgaacc tttattaaat tagtagttcc
    33241 acttactcct actggtactc cagcagttat aactactaat tccccattat ctatataatc
    33301 agcttgtcta gctgcctcaa ttgccttttc tataacttca tctgtatttc ctgcaacaga
    33361 agacttaact ggatatactc cccatgtaag agctaattta ttcatagtct tttcattatt
    33421 tgtagtagct attattggag attttggtct aaatttagat accattctag cagtatatcc
    33481 tgaagaagta gatgttatta tagctgatgc atttaaatct actgctgttg tacaagttgc
    33541 atggcttatt gcatctgtta cagtaacatt gttagtacca ttttccttta ataatctatc
    33601 ataatctaaa gtctcttctg ttctttttgc tattgtagcc atcattttta ctgcctctac
    33661 aggatatttt ccagcagcag tttctccaga taacattatc gcatctgttc catcatatat
    33721 agcattggca acgtctgtaa cttctgctct tgttggtctt ggatttctta tcatagaatc
    33781 aagcatttga gtggcagtaa caactggctt tgctaattca ttacattttt ttatcatcat
    33841 tttttgaact attggcatct cttcagttgg tatctcaaca cctaagtcac ctctagccac
    33901 cattatacca tcagaaactt ttaatatttc atctaggttc tctacacctt cttggttttc
    33961 tattttagat attatttgta tatcagttgc attattgttc tctaatactt ctcttattgc
    34021 tagtacatca gatgcctttc ttacaaaaga agcagctata taatctattc cttgacttat
    34081 tccaaactct atatcactta tatctttagg agttattgct ggtaaattta tcttaacacc
    34141 aggtacattt actcctttat gatttttaac tattcctgaa ttttcaacaa cacaaactat
    34201 atcttctcca tttatttctt ttactcttaa gccaactaaa ccatcatcta ttagtataga
    34261 atctcctgat ttaacgtctt cagcaagacc tttatagctt actgtacaca tttctttagt
    34321 ccccataata tctttcatag ttatagtgaa cttttgacct tcttctaaaa atacctctgg
    34381 gtcttcaaag ttacctgttc taatttctgg acctttagta tctaataata tagcaactgg
    34441 cttatttaat ttttctctaa ctttctttgc catatcaatt ctttctttat gttcttcatg
    34501 tgaaccgtga gaaaagttaa atctacacac atttagacca ttttgcataa gttgagttaa
    34561 aacttcctca ctttgtgatg ctggacctag tgtacaaaca atttttgtct tctttacatt
    34621 atttaacata tttgctcctc ctgagatatt attccacaga tttattttta atactaaaac
    34681 ttatatagat aatatttttg ccatctcata agcttcttta tcaaatactt tcttttgagc
    34741 taaagcttca cttatttcta aatcaattat tttgttttct tttattccta caactctagc
    34801 tgattttcca tctaataata attctactgc tcttactcca agtctacttg ctagtattct
    34861 atcagataca gttgggcttc ctcctctttg gacatgcccc aatacagtga ctcttaaatc
    34921 tgctcctgac tcttttttaa gttccttttc taaatcacta gcacttccga ctccttctgc
    34981 taaaacgatt atactatgtc tcttacctct tttttgagta gtctttaatc ttagagcaac
    35041 ttcatccact gtaatttcta cttctggtac tattatagtt tccgctcctc ctgcaagacc
    35101 tgcatataag gctaaatctc cacagtgtct tcccataact tctactatgt taactctttc
    35161 atgagatgat gatgtatctc ttatcttacc tatagcatct ataatagtat tcattgcagt
    35221 atcaaatcct atagtataat ctgtatatgc taaatcgtta tctatagttc ctggtatgcc
    35281 tatagctggg aatcctaagt cactaagttt ttgagctcct gcaaatgaac catctccacc
    35341 aattacaact aagcaatcaa ttttatactt tttaagaatt tttactgctt ttaatctccc
    35401 ttcttctgtt ttaaattctt cacatctgga agattttaat atagttcctc ccctgtgtat
    35461 aatatctcca acagaagata agttcatttc agttaaatct tcttctagta atcccttata
    35521 tcctctattg attccatata ctttgcatcc ataatatatt gctgatctta caaccgctct
    35581 aatcgcagca ttcattcctg gagcatctcc tccgcttgtt agtaatccta tagttttcat
    35641 tttttacctc cttggatttt atgtcaattt tttattataa tcattgttat tgtattctaa
    35701 tggacttttt ttgtccctat atcacttttt aatcccatca agactaaaaa aattaaatgt
    35761 ccttaaatgt ccattttgtg atattatatc acaaaacgga caatattttt catattatta
    35821 taatatattt agatttttat ataaaaaatc aagcttttac ttttatgtta tctttaggaa
    35881 gtaactcttc taagttattt ataaattcat cacatataca agtcaatgcc ccggtcattt
    35941 tatatgcttt acgtttacca tttatatttt catccatagg gtataagtat atgctgtcat
    36001 tacctggata ttgtttggct atttctacaa tattattagt tgtatcggta tcagacatac
    36061 tatctatttt caaatacaat tttttaccac tttctggctt aatattttct acatttctag
    36121 ttcttcctga attgtagttt ctatctgcaa agtcagtatc atcatttata tcttttatct
    36181 ctctagctat aagttttgca ttttcgcctt cttttatact taatgttcct tttacatata
    36241 ttatattatc ttcattcaaa atgacattat acttttgtaa taattgagga aaaactatta
    36301 tttctatagc accatacaaa tcttctaatt caataaatgc cataatttca tttcgttttg
    36361 ttgttttaat agttttagtt acaatcattc cacccattat aacatctgtt tcattcattt
    36421 gtaggtatga ctcttcttcc tcttttaatg aatttaattt tccattatcc atagatgttt
    36481 tttcttgtag ttctttttca aattgtgata aaggatgtcc acttacatac attcccaaga
    36541 cttctttttc aaggttaagt ctctctcttt cttcgtactc ttgcctttta ggtaaactgt
    36601 atatttgttg aaaatcacta tcttcctcac tatctccaaa agcatcaaat aaagaaattt
    36661 gaccttgaac atttttcttt ctatccatag atacactttc taataaactt tcataaccag
    36721 ccatcaaact agctctattt tcacttatat tatcaaaagc tccacatttt attagacttt
    36781 ctattactct cttatttgta tctttttggt ctaatctttt gactaagtct actaaatcta
    36841 caaataatcc attagattct ctttcagcga ttatattgtt tattatattt acaccaacat
    36901 tttttacagc tgctagacca aatcttatat tgtcgccttc aactgaaaac tttgaaaaac
    36961 ttttgtttat atctggaggt aatacatcta tttctagtac tttacattct cttatatact
    37021 caactacctt atcagtattt cccattacac tggttatcaa tgctgccata aattcaactg
    37081 gataatatgt ttttagataa gctgtctcat atgctaaaac cccatatgca gctgcatgtg
    37141 atttattaaa tgcatatcta gcgaagtcta tcatgtcatc aaatatttta ttagctattt
    37201 cctcttctac accatttctg atacaccctg caatttctat attaccatct tcatcaaatt
    37261 taccatgaat aaaatattgt ctttcttctt ccatcacgtc catctttttc ttactcatgg
    37321 ctcttctaac taggtcactt cgtccataac tataaccacc taaatctcta acgacctgca
    37381 taacctgctc ttgatatacc aaacaaccat atgtaacttc cattataggc tttaattttt
    37441 catgtagata tgttacatct tttgggttgc ttttattttt tatatatgca ggtatagaat
    37501 ccattggacc tggtctaaat agagatattc ccgctacaat atcttcaaaa ttgtctggtt
    37561 ttagctgttt cataaaactt ctcatgccag cactctccaa ttgaaacacc cctagtgtat
    37621 ttcctgatga aagcatttca tatactttcg ggtcgtcata atccatcttt gaaaagtcta
    37681 ttttttctgt gtagtttttt ataactctat ttttttctat taaatcaaga gtatctctta
    37741 taactgtaag agttcttagc ccaagaaaat ccatctttaa aagcccaagt tcttcaagtg
    37801 ttgtcatagt aaactgtgtt gtgattgcat cttgatgttt gtacaaaggt acatattcat
    37861 ctacgggatt ttttgatata acaactcctg ctgcatgagt agaagcatgt cttagcattc
    37921 cttctatctg ttttgatata tttattattt cttttgtttc cgggtcttgg tcatacaatt
    37981 ctttaagatt aggatttgta tctagggcct tatctattgt cattcctaaa gcaaatggta
    38041 tttccttggc taccttgtca actttattgt agcctatatc caataccctt ccaacatctc
    38101 ttattgcagc ttttgcaccc attgtaccaa aagttattat ctgggctaca tggtcatcac
    38161 catattttct ctttacataa tctataactt cttctcttct ctcataacaa aaatctatat
    38221 ctatatctgg catggatata cgttctggat ttaaaaaacg ctcaaataat agagagtact
    38281 ttattgggtc tatgtcagtt attttaagtg tatatgctac tatagaacca gccgcactac
    38341 ctctacctgg tcctactata atattatttt ctttagaaaa atttataaaa tcccaaacaa
    38401 ttaaaaaata ctctacataa cccatttttt caattacatc taattcataa tttaatctct
    38461 ccagtatctc attgctagga ttatcatatc tctcttttaa tccattaaaa cacaactccc
    38521 ttaaataagt ttctggtgta tatccttctg gaacatcata cttaggaaga tgtatagtat
    38581 taaaatcaaa ctccacatta cacatatcag ctattttcac agtattgtca atagcctcta
    38641 atgcatatgg aaataattct tccatttctt ctcttgattt taaataaaac tcatcactac
    38701 caaatctcat tctatttggg tcatttaatg tttttcccat ttgaatacac ataagaacat
    38761 catgtatttt cgaatcttct ttatttacat agtgaacatc attagtagca actagtggta
    38821 taccagtctc ttttgataat tttactaaac cagagtttac ttctttttgc tcaggtaaat
    38881 tgtgatcttg aatctccaaa aagaaatttt cttctccaaa tatgtctctg tagtcaagag
    38941 caaattgttt tgctttttca taatttctat tcattaaagc acgtgctaca tctcctgcaa
    39001 ggcatgcaga aagtgctata atccctttgt tatgtttttt aagttcttcc atgtcaactc
    39061 taggcttata gtaaaaacct tctacgtatg aagttgaaac tatttttatt aaatttttat
    39121 atccttccat attcttagct aacagaacta aatgtccttg atatttgtca tagtttgggt
    39181 ctttatctct caatccacgt gcagctgtat agacttcgca ccctataatt ggctttattc
    39241 cttcttttat agctgttttg tagaaatcta taacaccaaa catacaacca tggtcagtta
    39301 tagctattga gttcatgttt agttccttag ccctttttat aagttttttc accctcgaaa
    39361 acccatcaag taaactatac tcagtatgta catgaagatg agcaaaatct ttgttcatta
    39421 aatcacctcc tgctttataa ttttaatttt accacaaata tatgtactaa aacaaaaaca
    39481 gagactttta tctcaaaata ggaattaact tcaacgtata ccattatatg ttataaatta
    39541 atgcttattc tgagattgtc ccctcaattt attgctatga tttttttttc aaatctgttg
    39601 caatttcttc tatttttcta agtctatgat tgactccaga ttttcctatt ggtggttcta
    39661 acatctctcc aagttctttg agagtcatat cctcatactc gattctaatc ttagctatct
    39721 cttgtaaatt ttctggcaaa ctgctaattc ctatagtctc ttgtataagc ttaatatttt
    39781 ctacttgtct tactgctgca tttactgttt tagaaaggtt agctgtctca caatttacta
    39841 tcctgtttac attatttctc atttctttta ctattttagt attttgaaga cttaaaagag
    39901 cgtggtgtcc tcctataata ctcaacaaat ctgatatttg ttcactctct ttgatataaa
    39961 caacgtgatt attttttcta gccacaattt tactattaaa acctaaagag tttataagtt
    40021 cttttagaga atctgcaaac tcctcattat tagttacaaa ttccatatga tagttctttt
    40081 ctgggtcgct tatagaacct ccaccaagaa aagctcctct tatgaaagct ctcttacatc
    40141 cttcatgctt aattaagttc tctgaaattt tgtttatagt aaaaaatcct tctttatctt
    40201 caagtatacc taactttttt aataacattt ctgaacccat ttcgcttgtt accattaata
    40261 cataactgtt atttctcttt agcatttgat tcttatttac tgagattgta gtattgatat
    40321 taaaattctt ttttaataat ttaaatactt tacgggctat tgaatttaac tctgttgtta
    40381 tctttagatt tagcttctta tatccaacaa tttgtatact tccccctgat ttaactagag
    40441 ctgacaattc agctatatta caacattcat tcccagatat aactcttgct agttcatttt
    40501 tggtttcagt tgaaaaagac atatctatca cctattaact aatttttctt taaccttttt
    40561 tttatttctt gtaaggcaac ctttattatc tccttttcat catcatacct agctaagtct
    40621 actactctat ctaggtggat aaatttagca tcaataaagc cttcctcttt ttgtggaata
    40681 gtatccatat tctttgcttg catcaaatac caaaaaactg ttttatgaac tgctctattt
    40741 tcatcccagt tttctttgaa tgtatagtgt atttcaccta aatatttcaa tatatctgct
    40801 tttactccag tttcttcact tacttcacgt aatgctgcct cttggttatt ttccccttct
    40861 tctacttttc ccttgggtaa cacccaatct ccattgaact tccttagtaa aagaatcgta
    40921 ttgccaaaca ggactacacc accagcacta acctcttctc tcatcgatgt tcactcctta
    40981 aaactaacat aatgataagg atttctacac aattttttaa aatccttttt tactaacttt
    41041 tgttgtaatt atgatttagg gctaactcaa taactatgtt agatatatac tttgcatcat
    41101 gtcttatata attatttttt atttcaatta aattcttctc tacagttttt atacccatct
    41161 cttttaattt atctttttgc tttttatcta acaagacttg ctctgctcca tcttgtttat
    41221 ataagtcaag catttcttct ggcaatatct cattatttgc gattacataa tctattaaat
    41281 tctcatcagt gtgttctact atagcattaa catgctctaa tacattgtac ccttctgtct
    41341 cacctggttg agtcattata tttgctatat atacttttgg agctacagac gatttaatgg
    41401 cgtctacaat cccctctaca agtaaatttg gtattatgct tgtatataag cttccaggac
    41461 ccataattat aatgtcagca tcataaatag atgcaataac atcctttaat ggttttacat
    41521 cttttggatt taaaaaaatt ttatcaatag agcttttttg aatttttgac tcttcaggta
    41581 tactggattc tccatttatt atattgccat tttttaattt ggcaactaaa tttacatctt
    41641 ccaaagttac tggtaaaact cttcctgtta tagcaaaaat ttcacttaat ttatatacag
    41701 ctttttcaaa gtttccatac aacccattca tagcagccaa aaacaaattt ccaaaactct
    41761 gaccttttaa aagcccttct gtaaacctat attgcataac ttcattcatt gttggctcta
    41821 tatttgcaag tgctaataaa cagtttctta tgtctcccgg tgggagcatt cctaagtctt
    41881 ctctcaatac acctgaacct ccaccatcat cagcaacagt tacaattgca gttatatttt
    41941 gcgtagtgtg tttaagacct cttaaaaata ccgattgacc agtacctcca cctatcacaa
    42001 ccacatttgg ttctctattt tcaacaatag acctttgaaa tagttttact ttttcatttt
    42061 ttgctttttt atatataaat aacgatacaa ttaatgcaat ccaacctaaa attcctacaa
    42121 taataagtaa tttagccatg tttgcctcca ttatcgcaac atagagtctc tatgtttctt
    42181 tacaactcta tatcccttat tagataaatc ctccgctata agatttgtta tagtaactga
    42241 cctatgtctt ccacctgtgc atccaacacc aattactaga tgttgttttc cttcctctat
    42301 atattgaggt actaaaaaat gaatcatatc taaaagtttc acataaaatt cttcactgat
    42361 tttagaattc ataacataat ctctaacttc tttatcatca ccagttttag ctcttaactc
    42421 ttcaacataa tagggatttg gaagaaatct tacatcaaat actaaatctg catcagcaag
    42481 tataccatgt ttaaatccaa aagatactac agaaatagtt aggtttggat tatcttcacc
    42541 tgaagaatat attttcttta tttcttcctt aaggtcttta ggtttcatgt tagttgtatc
    42601 aattatacat gtagataact cctttaaagg ttccattatc tttctttcca tttttatacc
    42661 ttctggaatc tgcatatctt ttgccaatgg atgatttctt cttgtcattt tatatctctt
    42721 taacaataca tcatcattac aatccaaata taccatttca tattcataat tttctttttc
    42781 taaataattt aagctttcat gaagcgcttc aaaaaacttt ctccctctta tatctatccc
    42841 tagagcaact ttatcaatac ttgaatttgg ttgataacat aattctgcaa atttagttat
    42901 caaagcagga ggtagattat ctacacaata aaatcccata tcctctaaag ctctcattgt
    42961 ctcacttttt ccagatcctg aaagacctgt aactataaca aatttcattg ctaactcctc
    43021 ccttttatct tatgtttatt atattataat gttttctact aacgatatat ccagattaga
    43081 gtcagatata gtcttcatag ctagttcaaa atcaccaact ctttctggac aatgtgcatc
    43141 tgagcctatt ataaatttat tttctagccc ttttatttta tttaattgat ttacattcaa
    43201 aaaagaatgg ctactattta tctcaagtct agtatttgtc ttttttgctg tctttgctac
    43261 ttcttctata tacacatcgc ctttatctcc tggatgagta attataaaaa tatcattatt
    43321 acttatcgca tttattacag cttttgtatt atattctttt gctttttcag ttttaaaaat
    43381 atagttatta caatgattta ataagcttct taatgatgtt ggagtagaac caaagtgata
    43441 tccagccata atataatcaa aatctctaat aaatttatca tccatatcta tatttccttt
    43501 atcatctaat atgttacact ccatacctaa aagtatattt atatcagtgt acttatcatt
    43561 taaataatct acttcttctc tcattttgag aatatctttt ttctttacac catatgcaat
    43621 atgtttataa ctatggtcag atattcctat tgtttttatt cctttagaaa tggctacctt
    43681 tacattatcc tcaatagtcc ctttgccatg actatataat gtatgtgtat gataatcagc
    43741 cagtattttc atttgttaat cctctccaag tattttgact tcttcttcca acataacccc
    43801 aaacttggca tatacagcac tttttataac atatattaaa tccaaaatat ctttagcact
    43861 tgcttctcct tgattgacta caaatccaca atgcttctca gatacttgtg ctcctcttaa
    43921 agtaagacct ctcaatccag tttcttctat taatttacct gcaaaatatc ctgttggtct
    43981 cttaaatgta cttcctgcac tagcaaaatt taatggctgt ttagttattc ttctcgtcgc
    44041 taaatcatcc attaaacttt ttatttcatc ataatttcct tcttgtaatt ccaattctgc
    44101 tgataaagct atatatccgt ttttcgatat tatacttttt ctatacccaa attccatctg
    44161 ttcatttgat aattcaaata tattgccttc catatccatc agtcttactg acttcactat
    44221 gtccttcatt tcaccaccat atgctccagc attcatggct aatgctcctc ccaatgtgcc
    44281 aggtattcct gatgcaaact caaacccttt taattcttgc ttttggagag ctttccctaa
    44341 aactgataat aaagctcctg actgagcagt catcctagta tcatttatct cataagaatt
    44401 gaaattatca gctagttcta ttaccactcc tcttattcca ccatctttaa ttaaaatgtt
    44461 tgaaccattt ccaattatta aatacggaat agattcttct tttaccaact taagtacatt
    44521 ttttaattgt tcttcagttc taggtctaac taatatatct gctggtcctc ctaccctaaa
    44581 tgaaatatgt tttttcatag gttcatcgac ttttatatcc tctttactta atatatttaa
    44641 taaatttata tatatatctt gattattcat tgtatttcct cccaaattat actgaacttt
    44701 agctgtcatt attatttaaa ttacattagt atatagtatt aactatattc aataatatat
    44761 gattttgctt atcatatgga ttttatattt taagcttctt aattacaaac tatattcaat
    44821 aaatatataa taaagtaaaa aaaaaaaaat aacaacatat aaaagaatat aaataattta
    44881 aaaaaagcat caatatcact gatatcaatg ctttaaaagg caatttaatt cgtattattt
    44941 atctaactat ttttgacata gcaaagataa taaatccctc caactactat ggaacctcca
    45001 acgatatttc caagtgttac aaataccaaa ttagttataa attgaaccat agttatattt
    45061 gctcctaatg ccattcccat tggtataaaa aacatgtttg caacactatg ttggaatcca
    45121 cataatacaa atagcattat tactgtccaa catgaagcca attttgacat tatatcttga
    45181 gatgctgtag ccatccatac agctaaaacg actagaatat tacaaagtat tgcttttaaa
    45241 aacattgatg ctatagttga attaacctta gcttctgcca cagctatagc ttttgtagct
    45301 gcatctcctg tcaatgtaga agaataaaac acaagtgcaa ctagcaaaat tgaacctact
    45361 aaatttgcaa aataaaccag tccccagttt cttaacattt ttcctaatgt tatttttttg
    45421 tccataagtg caatagccat taaggtgttt ccagtgaata attctgctcc tgccactact
    45481 acaagcataa gacctactgg aaagaccata gcacttaaaa agctagctac ccctgggtct
    45541 atcttgccaa aagtttgact aactatcata ctccccaatg ccccaaaacc tataaatgct
    45601 cctccaaata aacctaatag aattgcattt tgtgtcgaaa tatttgcttt tttaatgcct
    45661 gcacttatag ttgcttgtgc agtttcccca ggtgtcaaga atcctttact cataaatcct
    45721 tcccccttaa aaattatact ttcccttgtc tttttatggc aataattata taataacaat
    45781 atgatatata ataaatttta ttaacataaa aaatatacta tatacttata tatacttgtc
    45841 tcatataatt atactattta ttatataaca actttgttaa tttcgaaata atttatattg
    45901 aatttgaaaa atttaccttt tgattattta ttttactttt taattattga ggtattatat
    45961 ataatagata tatgtttgtg ttattatata taattactta ttttaataaa tatttgaagg
    46021 agggaatttt atgtttgata tatcattttt cgaaatgtca atatatgaaa tagcagttac
    46081 tacaatatat ataattaatt tcatggttat actaaacctt atctttagag aaaaaagaaa
    46141 cataaataca actttaacat ggcttcttat attagtattg attcctgctc ttggattcat
    46201 actttatata gcttttggca gaaatatttc aaaaaacaat atgtttagat tgaaagaaaa
    46261 ggatgataag ataatcaaaa gtaatatact agatactcag gttaaactcc aatctacatc
    46321 tgaaattgat tcagatatac atcaacataa ggatatgatt tatgcattgg ctaattcaaa
    46381 taatgctcat tataccaata ataacgatgt gtggatttat gctgaatcta gtcaattttt
    46441 taacagtctt cttgaagaac ttaaaaaagc taaaaaatat ataaacatac aattttacat
    46501 tttcaaagat gacaaaatcg gaactgaaat aatagacatt cttatagaca aagctaaaga
    46561 aggtgttgaa gtaagacttt tatttgatgc agttggtggt agaacactaa aaaatagtac
    46621 actttccaga cttaaagaaa gtggtgttaa agttggaagc ttcttcccat cattcttgaa
    46681 aattgtaaac tttaacttaa actatagaaa tcaccgaaaa attgttgtca ttgatggtaa
    46741 ggtaggattt gtaggtggat ataatgttgg tgatgaatat ttaggtagaa atccaaaatt
    46801 tggtctatgg agagatacac atactaagct tactggtgat tgcgtaattg acctaaatat
    46861 gagattcatc cttgattgga gatatacaac taaagaagat ttagatttag aaaagtactt
    46921 tatagaagaa gatacaaatt ctaatcatcc ttctgaaaat attgggattc agatagtatc
    46981 aagtggtcca gatatatcag aacttgatga gattaaatat ggttacctta aaatgattca
    47041 gaaagctaga aagtatatat acatacagag tccttattta atacttgact caacttttat
    47101 agatacttta aaaatagctt gtttatctgg tgttgatgtg agagtgatga ttccttctaa
    47161 acctgaccat ccctttgtat actgggcatc atattcatat gctggagaac ttttaaagtt
    47221 tggagctaaa atctatactt atggtcaaaa tgcattttta catgctaaga caatagttat
    47281 agatgactct atatgctcta taggaacagc taatatggat atcagaagtt ttgagcttaa
    47341 tttcgaagtt aatgcattta tgtactcttc aaaaaaagca ttggaacaaa gaattatatt
    47401 tgaaaatgat attttaaatt ctaaggaaat aacattagat atatataatt ctaggtctac
    47461 ttatataaaa atcaaggaat caatttcaag actattatca gcagttttat aaatctttta
    47521 ttttataaaa attttacatt ttggtttatt ttttaaagtt ttttatatgt attatttttt
    47581 ttttattgaa tttagctcat tttttttatt ttttttaact attctgaatt attttatatt
    47641 ataatagact agtatagttt ttatttttaa taggtaactt ttttgcattg tttttataaa
    47701 taaagaaatt gttgtatatt attctttctt tattgttttg gttttgttca cccacataat
    47761 gttggggtca actcatggtt aattatttta tgaaaaataa taactttaaa aagaaaaggg
    47821 ggatttgtta tgtgcgattt tatttcacac aacaagcaat tatttgacga actagatata
    47881 tttattgact ctcttgcaac aaaggaaggt gcgttaatac aagttctaca tgaagcccaa
    47941 ggtatatttg gatatcttcc aaaagaagtt caacttcatg ttgctagaaa acttggagta
    48001 gctcctgcaa aggtatatgg ggttgttaca ttctattctt attttactac agaacctgta
    48061 ggtaagtata aaatcagtgt ctgtctagga actgtatgtt ttgtaaaagg tgctgacaaa
    48121 atcttaagtg cttttgaaaa acagcttggc ataaaagttg gagaaactac tagtgatttt
    48181 aaattttctt tagaaggttt aagatgtttg ggggcatgtg gtttagctcc agttgttact
    48241 gtaaatggta aagtttatgg taaagtgaaa cctgaccaag taagcgaaat attagatact
    48301 tatagagaat tagaattgaa ttgttaagag gggtgaataa ataatgcgta aagtaaattc
    48361 atttgatgaa ttaaaagttt tagctgatga gctgaaacct aatcttagct taagaaaaaa
    48421 ttattctaag gaaaatgtat taagaagaga attacttgtt tgttgtgata ctggatgtac
    48481 atcttcaaac agtctagaaa tagttagtga attagaaaat gaaataaaga aatcaggaat
    48541 ccaagataaa gttagtgttc gtttgacagg ttgttttggt ttctgtgctc aaggtcctat
    48601 tgtaaaagtt tatcctgata acgttttcta cgtaaaagtt gagccatctg atgctgaaaa
    48661 gatagttcaa agccacttaa ttagaaatac agttgtcgag gaattattat atgaggaaga
    48721 atctttaagt aaaaaagttg aagaacaaga agaaatgtct ttctacaaaa aacagttacg
    48781 tatagcttta aaaaattgtg gtcttataga cccagatagt atagaagaat atattgccaa
    48841 tgatggttat ttagctctag gcaaatgttt aacttctctt actcctcaag aagttattgc
    48901 agaggtaaaa acttctggcc ttcgtggtag aggtggtgca ggtttcccta ctggctctaa
    48961 atgggaagct gcttcaaaaa atccagctgg tccaacagat aaaaagtttg ttgtatgtaa
    49021 tgctgatgaa ggtgacccag gtgcatttat ggatagatct gtacttgaag gtgaccctca
    49081 tagtgtacta gaagctatgg caatatgtgg atacgctata ggttctgata caggatacat
    49141 atacataaga gcagaatatc caaaatctat agaaagatta aaagtagcca tagctcaagc
    49201 tgaaaaatac ggtttacttg gcgaaaatat cttaggcact ggatttaact ttaaattaga
    49261 attaaaatat ggtgcaggtg ctttcgtttg tggtgaaggt acagcgctaa tgcattcaat
    49321 tgaaggtaga cgtggtgaac ctagaatgaa aacttatagt tcttctaaga gtggtctatg
    49381 gaaatctcct acttgcttaa ataacgttga gacttttgct aacatacctg caattatact
    49441 taagggtgga gattggtttg ccaacttagg tactgaagat tctagtggta ctaaagtctt
    49501 tgctcttggt ggaaaggtag aaaatgtagg acttgttgaa gttccaatgg gtactacttt
    49561 aagagatatt gtatttgaaa ttggtggggg aatccctgaa ggtaagaaat ttaaggctgt
    49621 tcaaacaggt ggaccatctg gtggatgtat accaactgaa catttagaca ctcctataga
    49681 ctttggttca ttaagttcta tcggttcaat gatgggctct ggtggtatgc ttgttcttga
    49741 tgaatctgac tgtatggtag atatagccaa gttcttcctt gagtttactg ttgaagaatc
    49801 ttgtggtaaa tgtacacctt gtcgtatagg tactacaaga ttattagaaa tattaactaa
    49861 aattacagaa ggtaatggta ctctacaaga tttagatgac ctagaaaacc ttgctgaaac
    49921 tatacaaact gcttcattat gtggtcttgg taaagctgct ccaaaccctg ttttaagtac
    49981 tcttcaatat ttcaaagatg agtatatagc tcatgttgta gataagaaat gtcctgctgg
    50041 taaatgtcag aacttacttt cttactttat aacagatgac tgtaagggtt gtacaaaatg
    50101 ttctagagtg tgtcctgctg gttgtataac aggttctgtt aaggaacaac atacaataga
    50161 tacttctaag tgtctaaaat gtggtgcttg tatagataat tgtacattta acgctataat
    50221 caagaaataa cttatttgga ggaggaataa caatgagttt agttaattta actattaatg
    50281 gcaaacacgt ttcagcacct agtggaactt ctatcttaga tgctgcgaaa ttaataaata
    50341 taaaaataca caacctttgt catttacata tgaatgaaat agataagctt gacacttgtg
    50401 cttcttgtcg tgtttgtatg gttgagacag agagaggatt agttccagct tgtggaacag
    50461 ttataaagga aggtatgaaa gttcagacta atagtgctaa agcattaaat gctcgtagaa
    50521 ctattgttga attattgtta tcagaccatc cacaagattg ttttgtctgt gaaaaaaatg
    50581 gagattgtga attacagact atagctgctg atttaggagt aagaaaaata agatatcaag
    50641 gttctaaatc ttttgcaggt aaagatactt ctactaaatc tttagttaaa gaccattcta
    50701 agtgtatatt atgtagaaga tgcgagactg tatgtaatga catacaaaca gtaggagcat
    50761 tatctggagt taatcgtgga tttaatactt tagttagtac attctttaac tctgatatgg
    50821 ttgaaacaga gtgtacattc tgtggtcaat gtatatctgt ctgtccaact ggagcattaa
    50881 cagaagtaga taatgtacct aaactttggg atgttttaaa taaaaaagaa aagactatag
    50941 tagtgcaagt tgctcctgct gtaagagttg ctattggtga agagtttgga ttagaaccag
    51001 ggtcaatctc tacaggaaaa atggtagctg ctttaaaagc acttggattt gaacatgtat
    51061 ttgatacaaa ctttggtgca gattttacta taatggaaga agctactgaa tttatagaaa
    51121 gaatccaaaa aggtgaaaat cttcctatat taacaagttg ttgtcctgca tgggtaaact
    51181 tcttagagca caattatcca gacaagttaa acttagcatc tagctgtaag tctcctcaag
    51241 gtatgtttgg ttctatagct aaaaactact atgcacctaa aatattagga atcaatcctg
    51301 aagaacttta tgttgtatct gtaatgcctt gtgtagctaa aaaatatgaa gcttcaagag
    51361 aagaattaag tgaatctgga atactagatg ttgacctttc tataactact agagagttag
    51421 ctaaaatgat aaaagaagct gctatcgatt tacctaatct tcaagaccaa gattttgata
    51481 atcctctagg aaaatctaca ggtgctgcat ctatatttgg agcaagtggt ggagttttag
    51541 aagctgccct acgtacttca tatgagaaga taactaataa gactttagat aatgtaaact
    51601 tcactaatgt tagaggtctt aaaggaatta gggaagcaag tatagatgtt gatggaacta
    51661 cagttaatgt gtgtattgta aatacactta aaaacgctag aaagattatg gataaggtca
    51721 gaagtggaga atgtaaatac catatcatag aagttatggc atgtcctggt ggatgtgttg
    51781 gaggtgctgg tcaaccttat caccatggta atactgaaat agttgataga cgtgcaaatg
    51841 cgttatatga aatagataga aataaggcta ttcgtaaatc tcatgaaaat cctgatttac
    51901 aagctattta taaagacttc tttggagaac caaatagtga tgtagctcat aaataccttc
    51961 atactcatta ctttgataag agttgtgtct atggagagtg tccacaagaa tgtgcttgtg
    52021 aagaagctaa gtagtcataa caaagtataa aatttattct taaaaaagga ttgtcttaaa
    52081 atacttttac gtaatttaag acaatcctta ttaatttcac aaattaaatt atatttctaa
    52141 atagacttat tctttaatta acctatctat taattaaaga atacctgcca ttctcataag
    52201 ttgttttgca tttgtagttt tacatcttcc atcttttatc ttatagtcaa aatgaatttt
    52261 atcgtctaca tagtactctg agaaatgata gtttacaact tcaacatctc cactacctga
    52321 aaggtcacat agctcaaagt catgtgttgt caccatactt ataacccagg gttttgataa
    52381 tttctttacc gcctcacttg ctccaataat tctatcagca gagttagttc ccttaaaaat
    52441 ttcatctact agaaccaaca ttggcagttc ttttaaacta tattgaatca ttgattttat
    52501 tctaagaatc tcagcataga aagttgatat tccttgactt acatcatctt gtattctcat
    52561 agaagtaaat atagccatac aggtcgcatc aaaactctta gcgcatactg gagcacctgc
    52621 atatgccagc actaagttta caccgatact tcttaaaaat gttgtctttc cagacatatt
    52681 tgaacctgtg ataatacaag tctgagaatt taattgtatc gaatttgcta tgacactttt
    52741 ttctgctatt aatgggtggt atacctcttc aactttcaat cttggagtat cttcatattt
    52801 tattactgga aaacatgtat tttcctttac atgagaaaca actgttaaac ttattagagc
    52861 ttcaaattct ccaatagatt caaaccaatc tctaatttga tttccatata tacctttcca
    52921 tcgctccaaa gcttctgcac aatgatagtt ccacatcaaa acaccacagg caattatata
    52981 tagtatctga ttatacctta ggtttacagc atttcctatt gaatttaatt gtttaacacc
    53041 tcttgaaact ccacttccct tagaaagttt atcttgtaat tctttcaaat atgcactctc
    53101 aaacgtctct ttttctaaaa cctcaaacat tttttcatat acttgtatac ttctactaaa
    53161 tgaaaataat ggagcaagag tttgcataag tcttggatat ccaaaaccac taaaccctaa
    53221 ctggattata aaaagtaaaa aaacaggtaa aacaggtaaa aatcctaaca tacaaaatat
    53281 aaaagataaa attgttgcag ttggtagacc ccaagtaaaa atatgcatcc attttggtat
    53341 atacacattc ttattttctc catactctat aaacgattct atactactat ctgaattatt
    53401 tttttgctct ttaccaatta tattactgag tgtttgaata tgaatagaaa aatcatcttt
    53461 tgataaaagt tcttttatag ctctctgtct ttctaagatt acattctcat ctggattttc
    53521 attccacaaa tatttggcta aaaaattttt cccataagat gtattagcta cacagatata
    53581 ttgatacaat gatgctcttc caaataagtc taaatctttt gcttgcgagt tttcatcttt
    53641 taagtattct ttaccatcta ttttaaattc cttccaacca tctccaaatc tcttaatata
    53701 cttatttaat acttcgctct tacttttaaa atatgtttct tcttctttta ctttactatg
    53761 aataactaag agtgctataa atattattat tgataaaact ccaacaaaaa tacctactat
    53821 atttttattg taaaatccaa tagcaaaccc tgctatcact atcaaaaaag atattaatct
    53881 tgctgtactt atgttatttg actttttact taagagcaat tctttttcag tactactttc
    53941 taaaagttta ctaaaatatt ctattcccat aaaaaattcc aactttcttg ttatataatg
    54001 aaactcaaat ataaaattaa tctagtatta ttataatcta acactgtact ttaaattatc
    54061 cttatataat atatcttatt aataaacatt atatatatac aacttgattt taatttataa
    54121 ggtgtaggac ataatcctaa tcaatattta attaaatgat gtgtagttca ctattattca
    54181 gtactactta gtatcgatta attttgataa ttttttagta aattcttgag ctgcatcgta
    54241 tcccattcct ctttgtctaa aattcattgc tgctacttct aatatcatag cagtatttct
    54301 tccaggttta acaggtacaa ctaatttctc tacattaaca ccaagtattt cttcatattc
    54361 tttatccagt cccagtctat cataatactt agtttcatcc caagcttcta aatgaactac
    54421 taaatctatc attttagaat ttttgattgc ccctactcca tataaacttc taacatctat
    54481 tattcctatt cctcttatct caagaaaatg tcttattagt tcaggtgact gacccacaag
    54541 catactttca tctaattttc ttatttctac agcatcatct gcaactaatc tattacccct
    54601 ttgaatcagt tctaatgctg tttccgactt tcctatacta ctttcacctt ttatcagtac
    54661 acctatacca taaatatcta ctagcacccc gtgaattgtt gtgtgtggtg ctaacttatt
    54721 atccaaatag tttgacagtc tatttataag tctagtggta tttctatttg tgctcaaaag
    54781 tattctttta tattttttag ccttttcaat tacatctggc ttgatttcca agtctctaga
    54841 aattattaac gctggtattt catatgagaa aaatctatct agtacttctt ctcgaatatg
    54901 actatccatt aatccaaaaa aattatattc tgtctttcca actatttgta ttctttcata
    54961 tgcaaaataa tcaaaatatc ctgcaaactg taatcctggt ctatttatat cttgagagta
    55021 gacataataa tctacatcat caggcatgta tactactttt aacttcaact cttttattat
    55081 ctctcttatt gatactttat tgctagtttc cattaatttt cttcctttct aaaaaaatta
    55141 taaacattat ctgcaatgct tttattcatt ccatctactt ctaaaagttc ttccatagta
    55201 gcttttttta tagcatctac atctttaaag tgatttaata atgatttctt tcttttttca
    55261 cctattcctt gtatatcatc taagatagat tttgtcaaag ctttatttct tagacttcta
    55321 tgatatgtta ttgcataatt atgaacttct tcttgtatac tagcaataaa tctatataaa
    55381 ttagttgttt tatctagttc tatttctttt tctttgcata tcaatccttt agttctatgc
    55441 ttatcatctt tgtacattcc ccaaagaggt atatctacat cattgagttc tagtactttt
    55501 tttactgcac taacttgtcc ttttccacca tccaacaata tcaagtcagg taaatttcca
    55561 tgttttaatc ttctatctac aatttcagcc atagaatcat aatcattagg tccaataact
    55621 gtctttattt tatatctcct atattccttc ttatcttttt tagcattcgt atacactacc
    55681 atagaaccaa tcgaatctac accttgtata tttgatatat cataagcctc tattcttatt
    55741 ggcaactttt ctagattcaa tatttgcttt aattcttcca atgctccaat acttttttca
    55801 tattttcttt tattcatatc agaaaatttt tcaagatatt ctacagcatt tttcctaacc
    55861 atttcaacta agcttttctt ctctcccttt tgaggaactc ttattgttac tttttgcccc
    55921 tttttagatg acagccattc ttctaatata gaactatctt ctatctcatc ttctataatt
    55981 aattctttag gtatatactc ttgttcatta taaaattgtt ttacaaatga acttaatata
    56041 gaagctcttg gactatccat tacaccttct aatataaaat gttccctacc tacaatctta
    56101 ccatttctta taaaaaatac ctggacacaa gcttcattat gagctcttgc cattgcaacc
    56161 acatcttggt ttaagtcact tacagtagca tctatttttt gtttctgtat catctcttca
    56221 aggctcttta tcttatccct atatacagca gcctcttcaa atctaaagtt catagaactc
    56281 tcattcattt tttcctttaa aagttccatc aatttttctt ctttaccaga taaacacatt
    56341 ataatctctt ctatcatttt tccatactct tctttagaaa catttcctgt acaaggtcct
    56401 aaacatttat ttatgtgtag atttaaacaa ggtcttgtct tattctttat tgctttatct
    56461 atatctattt tacagcttct tattggatac gtactactta taagctccaa ggtatcattc
    56521 acagcagtta tattggtata gggcccaaaa tactttgcct tatctttaag aactcttcta
    56581 acttttaaga ttcttggata atcttcattc gtagttactt ttatatatgg ataagtttta
    56641 tcatctctaa gcactacatt atactttggt ctgtatcttt ttattaaatt acattcaagt
    56701 attaatgcct ctaactctga atccgttata atatactcaa acttatatat attcttaacc
    56761 atagatttta ctttagatgt atggtttttt gaagattgaa aatactgcct taccctattt
    56821 tttagagata tggcttttcc cacatatatt atatggtcgt atttgtcttt cattaaatat
    56881 actccgggct cagaaggtaa tttttttaaa tgctcttgta tatcaaacat ctcgccctcc
    56941 aaaacattaa attttatact aaaattatac cctaaaatat gaattttaaa aagttataca
    57001 atttccagtg ctttagacaa gttaaagccc atattccctt ctataattga tttgaaaaca
    57061 attttaacca tgaagagtaa tatggacttt actttgtatt actatttaca acttatattt
    57121 gactaattta cttataacaa aacagtttat cgtatatctg ctctaaaatt tttcacaatt
    57181 tatataactt aagaaaaata tttctttaag aattgacctg tataagaacc atcaacttta
    57241 gatacttctt ttggtgttcc agttgcaact atagttccac ccttatctcc gccttctggt
    57301 cctaagtcta ttacataatc acaagtcttt ataacatcta aattatgttc tatgactata
    57361 attgtatttc ctgtatctgc tagtctttgt aatacttgga ttagcttgtc tacatctgcc
    57421 atatgaagtc cagtagtagg ttcatctaat atatacaaag tctttcctgt tggtctttta
    57481 ctaagttcag cagctaattt tatacgttgt gcttctcctc ctgataattg agttgaaggc
    57541 tgtcctaatt taatatatga aagtcctaca tccattaaag tttctaattt tctttttata
    57601 tttggtatat tttcgaaaaa ctcaacagct tcttccacat tcatatctaa tatatcagat
    57661 atagttttat ctttgtattt tacttgcaat gtttctctat tgtatctttc acctttacaa
    57721 atttcacaag gaacatatac atctggtaag aaatgcattt ctatttttat tattccatca
    57781 cccttacaag cttcacatct accacctttt acattaaaac taaatctacc ttttttataa
    57841 cctcttgctt tagcttcatt agtagatgca aaaatatctc ttataaaatc aaatacacct
    57901 gtataagtag caggatttga acgaggtgtt cttccaatag gactttggtc tatatttata
    57961 actttatcta tactttcaat tccttttatc tccttatgct taccagctct ttgcttaagt
    58021 ctattcactt tacttgcaac acctttgtaa agtatatcgt ttattaatgt actttttcca
    58081 gaacctgaaa cacctgttat gcatgtaaac ttacctaatg ggaactttac atcaatattt
    58141 ttcagattat tttctgatgc tttaactatt tctataaaac ttccatttcc ttctctagta
    58201 gtctcaggaa gcaatatttg ttttttacca cttaaatatt gacccgttat tgagttctca
    58261 ttttcaatta tttcatctaa agtaccctgt gcaactattt caccaccatg tatacctgca
    58321 cctggaccaa tgtctataac ataatcagct tctctcattg tgtcttcatc atgctctaca
    58381 acgattaagg tatttcctaa atctgttaga tgtctaagag tagttataag cctatcatta
    58441 tctctttggt gaagccctat acttggctca tcaagtacat ataaaactcc aaccaatgca
    58501 gaacctattt gagtagctaa tcttatacgt tgcgcttctc ctcctgataa agttccagct
    58561 tttcttgata aatttaaata atcaagacct acatctctta agaacatagc tcttcctttt
    58621 atctctttta taatctcatg agctataaat ctctgttttt ctgtaagatt tatattttcc
    58681 ataaagtcaa tcaattcatt tacagataag tttgtaactt ccattatatt ttttccatca
    58741 actaaaacag ataaaacttc ctttttaagt ctcatccctt tacactttgg acatggtctc
    58801 tctgccatgt attcttcaat cttatctctc atgtaatctg aattagtttc tctatatctt
    58861 ctctctaagt ttactattac accttcaaag tatgatttat aatttctagt tcctccaaat
    58921 tttgactcat aagtaaactc tacaattata ttatctttac cataaagtaa ttcctccaca
    58981 aaatcatctg gtaaatcttt aaacggtgtt gtcaaagata cattaaaatg ctctgctaaa
    59041 ctttttatca ttttactgta ataagtgtca tcattaacac ctgttgaacc ccatgctgcc
    59101 actgcacctt gttttattga taaatctttg tttggtacta ctaattctgg gtcaacttct
    59161 ttactttccc caagaccatt acaaacatca caagcaccaa agggagcatt aaatgaaaac
    59221 attcttggag atagttcttc tatgccaatt ccatgttctg ggcatgcaaa tttagttgaa
    59281 aaaagaattt cttctccatc tattatttgg gctattacaa gaccatcaga taacttaact
    59341 gcagtttcaa ttgaatctgc tagtcttgat tctattccat ctttaattac tattctgtcc
    59401 actacaactt ctatattatg tttttgattt ttatttaaga ttatatcatc actaacttca
    59461 tagttttcac cattaactct aactcttaca aatccttctt ttttgatatt atcaaagact
    59521 tttttatgtg tacctttttg tcctctaaca attggtgaaa gaatttgtaa tttcgttcta
    59581 tctggaaaat ctagcatttt atcaactatt tcttgaatag tcatctgact tattttttca
    59641 ccacatgtag ggcaatacac atctcctact cttgcaaata atagtctcaa ataatcatat
    59701 atttctgtta ctgttccaac cgtagaacgt gggttttttg aggtagtctt ctggtcaatt
    59761 gagatagctg gtgataaacc ctctatatat tctacatttg gcttttccat ctgacctaaa
    59821 aattgtcttg cataagcaga tagactttct acatatcttc tctgcccttc tgcatatatt
    59881 gtatcaaatg ccaaagaaga cttcccagac cctgataaac ctgtaaaaac aataaattta
    59941 tctcttggta attctaaatc tatatttttt aaattatgtt cttttgctcc tcttattata
    60001 attttatctt ccataatgtt gtttttacct ttctattttt ccaatttttc ttctaattgt
    60061 ttcactttat ctctaagttc tgctgctctt tcaaattgta agttttgagc agcttccatc
    60121 atctctgatt ttaatttatc tatattagct cttatttcgt ctttatcatc tgtatcagat
    60181 attccatata caacttcttc ctctgcaacc ttagtagctt ctatactatc tcttatattt
    60241 ttctgtatag ttttaggaat gatattgtgc tcttcattat ataaattttg tatatctctt
    60301 cttctctttg tttcatcaat agcattttgc attgagtcag ttatcctgtc tgcatacata
    60361 attactcttc cattttcatt tctagctgca cgtccaatag tctgtattaa agctgtttcg
    60421 gaacgtaaga aaccttcttt gtctgcatct aaaattgcta taagtgaaac ttctggtata
    60481 tccaatcctt ctctaagtaa gtttattcct acaaggacat caaacttccc taatcttaaa
    60541 tctcttatta tttctgttct ttctaatgta actatatctg agtgtaaata tttaactttt
    60601 atccctattt ctttaagata gttagtcaag tcctcactca tttttttagt taaagtggtt
    60661 ataagcactc tttcattttt ttctatagtt ttagttattt ctcctaccaa atcatctatt
    60721 tgattattta taggtcttac ttctacaatt gggtctaaaa ggccagttgg tcttattatc
    60781 tgttctgcta ttgtctctga gtgttgaatt tcataaggcc ctggtgtagc acttacaaac
    60841 aacatttgat ttatatttcc ttcaaattct tgaaaattta atggtctatt gtcaagtgca
    60901 gaaggtaatc taaaaccatt ctctataagt gatgtttttc tagacctgtc tcctgcatac
    60961 atacctctta cttgtggtat agtgacatgt gcctcatcta caattatcaa ataatcatct
    61021 gggaagaaat ccattaaagt atatggtctt tctccttctg accttcctgt aatatgtcta
    61081 gaatagttct ctatcccctg acaaaatcct atttccttta acatttctat atcatactta
    61141 gttctttgtt caattctttg tgcttctaat agcctatcat tttccttgaa aaatttaatt
    61201 tgttcttgaa gctcgttttc tatttctaca attgcctttt caattctttc tggcgttgtt
    61261 acatagtgag atgctgggaa tataacaaca tgatttctag taccaacaat cttccctgtt
    61321 acataatcaa tttctgttat tctatctacc tcatcaccaa aaaattctat tcttattgct
    61381 ttttcatcat tactagctgg gaatatttct agtatatctc ctctaactct aaaggtacct
    61441 cgtgtaaagt ttatgtcatt tctttcgtat tgtatctcta ttaacctttt tattacatca
    61501 tctctgtctc tttgcatacc tggtcttata gataacataa gttctttata gtcttttggg
    61561 tcaccaagtc catatataca agatacagat gatattatta tagtatctct tctctctaaa
    61621 atagatgctg tagcagaatg tcgaagttta tctatttcat catttatgct tgcatctttt
    61681 tctatataag tgtcactgtg agctacatat gcttctggtt gataataatc atagtaactt
    61741 acaaagtatt caactgcatt gtctgggaaa aattctttaa actcactgta aagttgtgct
    61801 gctagagttt tattatgagc cattattagg gtaggtttct taacttgttg aatgatattt
    61861 gccattgtga atgtttttcc tgaacccgtt acacctaata atgtcgaaaa tttctcattt
    61921 ctatttatag aatccactat tgatttaata gcttctggtt ggtcacctgt aggtttgaag
    61981 tctgatttta ttttaaaatc catagtatca ctcctttttt cgaacattag ttcctgacat
    62041 gtattattat atcatacctt tataaaattg tttaagttat tttaaatatt ttacccattt
    62101 aaattttact taaatctaaa acataaaaaa agacttctat ctttgcaata gaagtctata
    62161 attcatggaa aataaatact ttattcaact tacataataa aatcaaatgc ttctttttca
    62221 actccatcta attttaaatt aaacattttt aatatagttg ctgcatgtgc tctcaggtct
    62281 ccttcttcta taaccacacc acctttaata tttggtccaa aagctataaa tggaggtcta
    62341 ttacctttag taggtaaatg accatgtgtc gcaacagcaa acttgtaatc ttttatatct
    62401 gaatctttta taatacttcc ttcgaaatca tttccaaatg aaattccttc aagaccttca
    62461 attaccattt caaaatcacc ttcaagacca aggttagatg cttcctcttt ggtaaaaata
    62521 ttctctatga ataattcttc tttaaattct tcaagcagtt ctcttaattc tgttaaccta
    62581 tctaagttat ttactattat ttgagctgat attccagaag aatttacata ggcatcaaaa
    62641 tccttaactt ctccattttc aacttttata aatccatttg atttaagcaa gacatttgga
    62701 tttataactt tatcaactct aagatgacca tggtccccaa gtattataaa atttgtatct
    62761 ttgtatgtac ctgcatcctt tgtagcctga ataatatctc ctaaccattt atcattcatt
    62821 tttaatgctt tatctacttc gtcattaaat aagccataat tgtgtcgtgt atggtccaat
    62881 gtagctaaat gtattagcat taaatctggt ttatattcct ttattatatc tactgaacag
    62941 tttactccaa ataaatccat attgggttct aatttccaat cgatataatg acaatgtcta
    63001 ttgtaaatac cgtccattat aaccttactg gaacttcgtt caaaagtttc tcttgggtca
    63061 tcttctctac taggagccca tatttctgca atattataat ctatatttgg atttgctccc
    63121 attacaggcc ataatacact agaggtagtt agattatttt cttttgctac atctataata
    63181 gtttttgctt ttatgtcctt tgagtaccag taccagtctt tattttcttt atttgggtca
    63241 aatttttcat tatgatatat accatgtttt ttaggatata ctcctgtaac catactagta
    63301 tgacacggat atgtcagagt gggataaaca caatttatat tttttactat ggagcaattt
    63361 tctagtatag atttaaagtt tggtagattt tttacaaatt ccaagtcttt ttcaaataaa
    63421 gaatctactg ataatactat aacctttgtc atttacatta cctcttcatt tatatattat
    63481 tgaatacttt taatcaactt acaagtctat ataattattt atttgtttta tctattactg
    63541 cttgcattgc agaagttcct ttgcttacta catcttttgg atttgcattt aaatcagtaa
    63601 gccccttttt aatctcatcc attagcttat cagttgcttc cttgtatcca tttaacattg
    63661 gaagtgcaac agcatattgt aattggtcag ttgcaacctt atattgaggt ttttctttat
    63721 ataatgtttg taacttttct gagtttgttg catcttcagt tgttggtaag tatcctgttg
    63781 atgaactaaa ctgaacgatt ttatctgttt gtgtcatcca ttctataaat tctgcacttg
    63841 cttctttttc tttatctgtt ccaccttcaa gcataactag attagctcca cctgtaggag
    63901 ttgcgtattt ttgatttttt ggtaagaatg ctgttgatat ttcaaactta ccttctgttt
    63961 gttgcataag tcctgctaaa tctgctgttg atgtaaatat catacctact ttaccattta
    64021 caaaatcttg ttttgctacg tcccaggcat tataatcttc tccaggaggc attttcatta
    64081 ttccagcttt catcatatct tgccagaact taactggtgc ttgtcctgcc tcattattaa
    64141 atgctacttt tttaccatta gttatttctc ctccactttg cattaccata gcttcataga
    64201 accaaatatc tattggcgtt tcaaaaccat aagtatcttc tgatttattt gtcattttac
    64261 tagcataatc cttaagctct tcccatgatt ttggacctga agggtcaaga cctgctttag
    64321 ttgccatatc tttatttatg tataatattg gtgtacttct attaaatggc ataccaacaa
    64381 acttatcctc ataatatgaa tttgttaaaa gaccagaaaa gaatctttct tgatatgttt
    64441 tatcaaaata tttactcata tctactaaag cacctgctga tgcgaatgta cctgtttgag
    64501 ttatttctaa taatgttaca tctggttggt ctttagatgt taaggctgcc tgtagttttg
    64561 ttgcattttc atagtaatct ttctgtttta acatatttac ttgtacatcc ttatgttcac
    64621 tattaaattg gtcaaccatt ttttgagcag tttctccatt agttccacct aaaggagccc
    64681 agaaatctat cttcgtaata ttgctatcag tcttttctcc tttagatgca tttgcatcat
    64741 tagaatttga acacccaact aaagaaccta ataataatgt tattgctaat cctgttgcta
    64801 attttctttt aagcttcatt ttcatcctcc aaatttagtc tttaattttt tattttattc
    64861 ctgaatacat aaaagcttct tttacttttt tattcgcaaa tatataaaca agaattattg
    64921 gtaaaattag aattatattt cctgccatta ttacattcca tgcagtaatt ccttctgaag
    64981 atttaaggag cgcaactcct attggtaaag ttcttattgc atctgtattt gtcattacta
    65041 agggccagaa ataattattc caatggtata taaaattaaa taatataaat gttattactg
    65101 ctggttttgc cataggaacc attattctaa acattatttt taagtcagaa gcattatcta
    65161 gtttagctgc ttcaataatt tcatctgaaa tctgcataaa attctgtcta agcagaaata
    65221 tcccaaatga agatgtcata aatggaatta taagtggaac ataagtattt ataaatccaa
    65281 acttactcat catcatatag actggtagaa aagttacttg tggtggaatc ataagtccta
    65341 caagcaaaag cccaaatagc attttttcac ctttaaattt aatcttagaa aatgcatatg
    65401 ctgctggaac cataactaaa aattgtacaa ttaaaacact tcctgttaca aatacactgt
    65461 ttagcaaata tctaaaaaat ggacctgaat gccatgctgt actataattt tctaacacta
    65521 tatcatttgg tataatagtg ggcggtaatt gcatcatttc ttgtggattt ttaaaggatg
    65581 tagatagcat ccatataaaa gggaatataa atattgcaga taaaagtaat agaattaata
    65641 tttctattga ttttgatgct atttttttta tatcaacttt gtcttttttg ataggttgta
    65701 taatatccat tgtctcctcc ttttggataa accttataat atctactttt tctttattga
    65761 taatgaactt tctttgataa gagtctaaag ttagcatatg tagcaatcat aactatagct
    65821 aacaaaaaca tactcgctgc actcgcttgt cctatgtcat agtatataaa tccagactca
    65881 tatatataat aagataacat gttagttgaa cccactggcc caccttgtgt cattatactt
    65941 atctcatcaa atacttgaaa agatgcagtt gttgttgtta tcataagaaa aaatattgtt
    66001 ggtgataata gcggcaatgt cactttaaag aaattcgtta ttttgtttgc attatctaat
    66061 ttagatgctt catatatata gtttggtatg gatttaagac ctgataacac tattaagata
    66121 ttgtatccaa gagtcttcca tattcctatt agaattagtg acattaaagc tgaactttca
    66181 cttgaaagcc actttgatgt tggtaaatgt aatgtcgata gtatccagtt taaaaagcca
    66241 tattgtggct ccataatcca aagccacaat acagctacag atactagtga aattacatgt
    66301 ggtgtaaaga ttatactttg cgttaaattg tgtattatag aatttttatt tagccaaaca
    66361 gcaagtacta tggctagtga tatagatata gtcaccataa aaaaagtata aattgcagtg
    66421 ttcgttatag tttgtataaa ctctggattc tttaaaagtt ctgtataatt gtttaagcct
    66481 acattttcaa aggttgaact aagaagacta ccagatttga agcttagaga tatcgtctta
    66541 attattggat agactataaa caataaagtt cctataatag caggtgttat atataaatat
    66601 gctttattaa attgcttttt cataaatatc tcctttaatt ttagaataca tacttttaca
    66661 tacttttaca tcttctattc tttctccatt tttatcaaaa taatataaat ctttttctaa
    66721 tacacttatt cttaaagttc ttccaatatc aaatatatca ttttccgttt tgactcttat
    66781 ctctctatta agaacagata aacaatatat agtttcattt cccaacactt ctttagtcac
    66841 aacttttcca aataggctaa atacatcatc atttttccca tctatgttta tactctctag
    66901 tgttgctttt tctggcctga atccaaattt aatatcattt tttatatcta gtatgttcat
    66961 acaaggactc cctataaatt gagctacaaa taaattttta ggattttgat atatcttctt
    67021 tggggaacct atttgcatta tctttccttt gttcattata actatcttat ctgccattga
    67081 cattgcctca acctggtcat gagttacata aacaaatgta gactttagtt tttgatgtaa
    67141 ttgtattaat tctgttctca tttgatttct taatttagca tctaaatttg acaatggttc
    67201 atccattaaa aatacctttg gatttttaac catagctcta gcaagtgcca ctctttgtct
    67261 ttgacctcca gataagttgc ctggtttcac atttaaatat tctctcaatc ctactacatc
    67321 aataacttct gttataagtt tttctctttt ttctttttct acttttttgt ttttaagacc
    67381 aaattctata ttttctctaa cagtcatatg tggatataat gcataatttt gaaataccat
    67441 agctatatcc cttttactag catccgtttt tgtaacatca ttttcttcaa tcttcaacct
    67501 acctgaagtt atatcttcaa gacctgcaat cattcttaac gtagtagatt taccacatcc
    67561 acttgggcct accaatactg taaaagaacc atcttctata gtaagattta aattttctat
    67621 aactgtattt tttttattat aagattttgt tacattttct agtcttatat ctgccatttt
    67681 tatcctccca aaatatttaa tttaattcta cgatttactt atagattaat aatactctta
    67741 atttgtaaag gatatattaa gacaaagtga atatatggta aacttattaa aataaaggtt
    67801 aaattataaa cattttttgt taaatatact gaggttaaga tgatatacag cttatgttag
    67861 ctataatttt ctatattttt catattcatg gtttgctatc acaatgtaat tttaatttat
    67921 gaagagtcat ataaaactat ttatttttta tataatttga tttgaaagta aaaaagacct
    67981 ctatggggga tagaggtctt taagtggggg agattgagta atttattttt gctatattaa
    68041 gttttacaat tatattatgt cccaaattta caaggtttaa acataaaaaa taaaactttt
    68101 ttaattttta taaatttacc caaaaaaaat tacgtggcta cgtccta
//
LOCUS       DAGSWI010000003        65432 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.003, whole genome shotgun sequence.
ACCESSION   DAGSWI010000003 DAGSWI010000000
VERSION     DAGSWI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 65432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.003"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(59..382)
                     /locus_tag="MJF80_000119"
     CDS             complement(59..382)
                     /locus_tag="MJF80_000119"
                     /inference="COORDINATES: protein motif:HMM:NF020435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBZ0278807.1"
                     /translation="MLLVESKNDRSILAKESLEKLGAKNVKIYNMDTGSNVIYSVENG
                     KEHLTISNEKRFPNWNEIKYVRYKLMKPNKTIVQILPPKEEYINLHENCFHLWEIEDN
                     AVPTK"
     gene            complement(400..573)
                     /locus_tag="MJF80_000120"
     CDS             complement(400..573)
                     /locus_tag="MJF80_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278808.1"
                     /translation="MTNKEMCKSKNLDEREVYKEFGKEICASCINNRRDCESKDCDIA
                     YENWLEKETKNYL"
     gene            complement(578..817)
                     /locus_tag="MJF80_000121"
     CDS             complement(578..817)
                     /locus_tag="MJF80_000121"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278809.1"
                     /translation="MKKYKIKFEEKVTLEHEVIVEIPNEININDICNCIENKCQRVYD
                     IADYIREFNGRQIDFTEDTCGETEMVVESFRKCKE"
     gene            complement(913..1188)
                     /locus_tag="MJF80_000122"
     CDS             complement(913..1188)
                     /locus_tag="MJF80_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278810.1"
                     /translation="MEYKEYEDLKNRVESYEDLQGSAEFAERVIENLEDVDCPIRIGF
                     KFPNKEDYQKIDLDIAAKDSNSTFIRTELAKAFKEILSKYEMDMENM"
     gene            complement(1240..1398)
                     /locus_tag="MJF80_000123"
     CDS             complement(1240..1398)
                     /locus_tag="MJF80_000123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278811.1"
                     /translation="MDDRLEICKKMFPIVTKNTRLLCHYCDGRNICSYDEEKAHELLR
                     NEEGTKNN"
     gene            complement(1501..1950)
                     /locus_tag="MJF80_000124"
     CDS             complement(1501..1950)
                     /locus_tag="MJF80_000124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278812.1"
                     /translation="MSLIKYRGYDFENEKWIYSETIKWSDAVDCLFMLKENCEWQKVS
                     NVGVFSKCWSGNNEEIYEGDILKEPYNTKNKYEYGIVRQENYALELYVEWHYLKQFEG
                     KWEEITSKATIMNSKKYVVVGNECENLEEIKKEFLERKEMLENEHLS"
     gene            complement(1937..2086)
                     /locus_tag="MJF80_000125"
     CDS             complement(1937..2086)
                     /locus_tag="MJF80_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278813.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIAIKILSKYECEGMI
                     RNESN"
     gene            complement(2055..2435)
                     /locus_tag="MJF80_000126"
     CDS             complement(2055..2435)
                     /locus_tag="MJF80_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278814.1"
                     /translation="MAKIWLDAGTFLEKTIDIEDMFELNLRKVRKANESKKIKLKLNE
                     SKFKKIRKKPTNDTKSKPIKVFNIETGEVRIFKSAKAASKYLKISGDYASCLARENRV
                     TREGWKAEYIQEVSDGISKCGASN"
     gene            complement(2453..2674)
                     /locus_tag="MJF80_000127"
     CDS             complement(2453..2674)
                     /locus_tag="MJF80_000127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278815.1"
                     /translation="MRFEIGKTYKFDKEKFMEINGVEQHKKYKELWIDDIEGVEFTVE
                     KTFDDGYVCYPNEFWFNFGVVSEWCVEVK"
     gene            complement(2679..2840)
                     /locus_tag="MJF80_000128"
     CDS             complement(2679..2840)
                     /locus_tag="MJF80_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278816.1"
                     /translation="MSRTAICSVCGEFLVESYDDQFTNTKVMDLHGREFVIVICKSCL
                     EELLRKCED"
     gene            complement(2910..3317)
                     /locus_tag="MJF80_000129"
     CDS             complement(2910..3317)
                     /locus_tag="MJF80_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBZ0278817.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWKSITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
     gene            complement(3333..3506)
                     /locus_tag="MJF80_000130"
     CDS             complement(3333..3506)
                     /locus_tag="MJF80_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HBZ0278818.1"
                     /translation="MARIYAQRSGSLNEQDRLELLRLLGKAGYTVKVAREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            complement(3567..4448)
                     /locus_tag="MJF80_000131"
     CDS             complement(3567..4448)
                     /locus_tag="MJF80_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBZ0278819.1"
                     /translation="MGIIRVSKDKDNPYVVLNKTCLEDVKLSWQAKGLHSYLISKPDH
                     WKIYVNDLYKRSKNGRDATANILRELIENGYITRTPCRDSNTNKMLGGYDYQVYEIPL
                     ENPQKLKSRKTDFPETGFPGNRVSRKPENTEVVSNDFKVNNDITTIVINEQSNKDKTT
                     YIKKYFEKYIGVITPNNFIELMSYLDDGMEADVIIRAIDEAIANGVKNYKYVKTILNN
                     WIEAGVKTNLELTEYQNEFERKKKNKQDKKQSNSKAVNTPNVSKNKFHNFNETFTQYS
                     PDELDDIIKKSQKVKFK"
     gene            complement(4463..5047)
                     /locus_tag="MJF80_000132"
     CDS             complement(4463..5047)
                     /locus_tag="MJF80_000132"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBZ0278820.1"
                     /translation="MEINNIYIKLMDVRVKFSKLNLKKSGENKFANFKYFELADFLPQ
                     ATGLLEEAKLCPIVTFTNEYATLTLINGENPSEQIVFTSPMRDLQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITESDTFDATSGKNEAKSNSNNRILTDKQLSRLYAIASNANVDK
                     ESLKEKVFKRFGKEIKDLTKQEYDTICNAYENKQ"
     gene            complement(5057..5566)
                     /locus_tag="MJF80_000133"
     CDS             complement(5057..5566)
                     /locus_tag="MJF80_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBZ0278821.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKKVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            complement(5644..5961)
                     /locus_tag="MJF80_000134"
     CDS             complement(5644..5961)
                     /locus_tag="MJF80_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278822.1"
                     /translation="MKSLTIVRNAVEQQLNRANLEINKNEELYTKLRKKEKRDVLDEI
                     ELSNALREKSVNERLKIFAESLLKIIDTQIEIKEYEESEDYKIFELISEEIERDRPID
                     IQI"
     gene            complement(5964..6110)
                     /locus_tag="MJF80_000135"
     CDS             complement(5964..6110)
                     /locus_tag="MJF80_000135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278823.1"
                     /translation="MNVRVLIAYVQFCNDKQIKASFEGLRKYNRDIELVNLYLPSKLK
                     IKGD"
     gene            complement(6111..6554)
                     /locus_tag="MJF80_000136"
     CDS             complement(6111..6554)
                     /locus_tag="MJF80_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0278824.1"
                     /translation="MSKLGNISNFNLDKQEDKSFNDLDNISICFSEGIRKVVEMNLNN
                     YKNKISKYLNETSKIELLEPKELTIVISKGYPDYLMSVEEASKRLKIDKVFGYELIKN
                     GLLKSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKEIREGVI"
     gene            complement(6584..6781)
                     /locus_tag="MJF80_000137"
     CDS             complement(6584..6781)
                     /locus_tag="MJF80_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0278825.1"
                     /translation="MAINDNINKILKDRDLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKIADYLDITLDELVGR"
     gene            6834..7034
                     /locus_tag="MJF80_000138"
     CDS             6834..7034
                     /locus_tag="MJF80_000138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278826.1"
                     /translation="MKVGDIMQFSKDILHTLTLEILKEKYDFKSSSEEELLKHYHEIF
                     LKLSEVNNSFSKGDGLSVFKQM"
     gene            complement(7021..7218)
                     /locus_tag="MJF80_000139"
     CDS             complement(7021..7218)
                     /locus_tag="MJF80_000139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278827.1"
                     /translation="MKISFESVVNNVNNETELILSKEELQIAKRILNTLNENEQSILS
                     SKDILDFCKEALKYNLVPTFV"
     gene            complement(7278..8069)
                     /locus_tag="MJF80_000140"
     CDS             complement(7278..8069)
                     /locus_tag="MJF80_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBZ0278828.1"
                     /translation="MNNLQIFEKLEFGQIRMVEVDKKPYFVATDIAKCLGYANTSKAI
                     NDHCRWVTKSYIPHPQNENKILEVNTIPESDMYRLIVNSKLPNAEKFESWVFDEVLPT
                     IRKTGGYIHTTEDMTDDEIMARALQVAQKTIEKKSREIEEKDKVIQLQQPKVLFADAV
                     ASSDDSILVGELAKLLKQNGIDTGEKRLFAWLRDNGYLIKRKGEDYNTPTQKSVDLKI
                     METKKRAIYNPDGSTKITKTPKVTGKGQIYFINKLKLVNQLSMLS"
     gene            complement(8072..8242)
                     /locus_tag="MJF80_000141"
     CDS             complement(8072..8242)
                     /locus_tag="MJF80_000141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278829.1"
                     /translation="MEKVRMTVRLVPKLNQYVQNIAKECGKSKNSIIVDACWEFIEKI
                     KKENKFNVESEE"
     gene            8363..8674
                     /locus_tag="MJF80_000142"
     CDS             8363..8674
                     /locus_tag="MJF80_000142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278830.1"
                     /translation="MTTITVRIYTPLNENLEKISYQTGILKSSLILYAINDIIRNSKV
                     NELQSISYKSDDTVRSTLRIPGVLKELLEKTAKENNLSVNSLINNAVHSFCISHWLIY
                     L"
     gene            complement(8660..8797)
                     /locus_tag="MJF80_000143"
     CDS             complement(8660..8797)
                     /locus_tag="MJF80_000143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HBZ0278831.1"
                     /translation="MKHEQTTLRIPEDLYKALIDLSKEIGMPIASIVIIACWLYISKI
                     N"
     gene            complement(8852..9640)
                     /locus_tag="MJF80_000144"
     CDS             complement(8852..9640)
                     /locus_tag="MJF80_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBZ0278832.1"
                     /translation="MKNLTIIKQNNQFLVESREVAELIEKKHDNLLRDIRGYKKILED
                     SSNLRSQDFFIESTYINTQNKIQPCYLLTKKGCDMVANKMTGEKGIIFTAIYVTKFEE
                     MEQELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADSVA
                     SSDNSILVGELAKLLRQNGIDTGQNRLFDWLRNNGYLIKRKGEDYNTPTQKSVDLGVI
                     ETKEGTRVHPDGHTSITKTPKITGKGQIYFINKFKSSKQLSMLS"
     gene            complement(9609..9872)
                     /locus_tag="MJF80_000145"
     CDS             complement(9609..9872)
                     /locus_tag="MJF80_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBZ0278833.1"
                     /translation="MYLNRLEGLMKENRHTQKNVADILGLSSYGFRLKLKGKNEFKAS
                     EIKKISKLYNVSADYFFSDEVAKIAIKEERGNNNEESNHNQAK"
     gene            10051..10446
                     /locus_tag="MJF80_000146"
     CDS             10051..10446
                     /locus_tag="MJF80_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0278834.1"
                     /translation="MNRIKELREEKGISLDKLSEDLHINKSTLSRIENGLREPKKSTI
                     EEYANYFDVSTDYLLGRTDVRNSLFINKNEKDYDAENFKTEKELIENMYLDEDMKEVF
                     NIFSELSPDAREKALKVAELFLLDEKNKK"
     gene            complement(10483..10620)
                     /locus_tag="MJF80_000147"
     CDS             complement(10483..10620)
                     /locus_tag="MJF80_000147"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278835.1"
                     /translation="MNKTKYYDILKLNLLMKKLKELDKNKINEYKIKVKEIHKINKKE
                     E"
     gene            10904..11125
                     /locus_tag="MJF80_000148"
     CDS             10904..11125
                     /locus_tag="MJF80_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0278836.1"
                     /translation="MLKELRKKKKLTQIELAKRVGCHRSQISRLENNEDKDLTISILI
                     ELEIALGLEEKYLVNYFADEYIKKRKLHK"
     gene            11184..11390
                     /locus_tag="MJF80_000149"
     CDS             11184..11390
                     /locus_tag="MJF80_000149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278837.1"
                     /translation="MNLSFNKKKKFELSKEELELIENWLGNKYAGDMTIPAIVDFSLE
                     TDIEYEKIVVYLAEKVLESRDKKH"
     gene            11527..12693
                     /locus_tag="MJF80_000150"
     CDS             11527..12693
                     /locus_tag="MJF80_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBZ0278838.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVDGKRKKVERVGGNTKKEAEKSLR
                     DAINEYENAGIVFDETNMSLSDYLNFWYKEYVLLNCKYNTQESYRNLIENHIEPRLGK
                     CKLKSINPAIIQEFLNNKSKETYTQNGEEKHYTKGVLKAIYVVLNAALKSAVYPYKLI
                     KENPVQYASIPKNVLKVKNESDNKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMRK
                     GEILGLCWDNVDLDNNIIKVRKNLIKRKVSEFELASPKTKTSIRDIKIGDTLSRILKE
                     EKLNQKKQKIKIGKWYKETEYDWVCRKKDGSFVNHNNIDACVRTINKKLNLDFNFHCL
                     RHTHATLLLENGANIKYIQQRLGHSQLSTTMDTYSHVTSKMESETVDILEGILQ"
     gene            complement(12603..12987)
                     /locus_tag="MJF80_000151"
                     /pseudo
     CDS             complement(12603..12987)
                     /locus_tag="MJF80_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(13227..14621)
                     /locus_tag="MJF80_000152"
     CDS             complement(13227..14621)
                     /locus_tag="MJF80_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBZ0278839.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(14641..14850)
                     /locus_tag="MJF80_000153"
     CDS             complement(14641..14850)
                     /locus_tag="MJF80_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBZ0278840.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(14879..16324)
                     /locus_tag="MJF80_000154"
     CDS             complement(14879..16324)
                     /locus_tag="MJF80_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBZ0278841.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            16434..17228
                     /locus_tag="MJF80_000155"
     CDS             16434..17228
                     /locus_tag="MJF80_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBZ0278842.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            17279..18601
                     /locus_tag="MJF80_000156"
     CDS             17279..18601
                     /locus_tag="MJF80_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBZ0278843.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(18701..19918)
                     /gene="cdeC"
                     /locus_tag="MJF80_000157"
     CDS             complement(18701..19918)
                     /gene="cdeC"
                     /locus_tag="MJF80_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBZ0278844.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(20101..20281)
                     /gene="ssrS"
                     /locus_tag="MJF80_000158"
     ncRNA           complement(20101..20281)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="MJF80_000158"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(20396..21301)
                     /locus_tag="MJF80_000159"
     CDS             complement(20396..21301)
                     /locus_tag="MJF80_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278845.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            21513..21911
                     /locus_tag="MJF80_000160"
     CDS             21513..21911
                     /locus_tag="MJF80_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278846.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            complement(22034..23050)
                     /gene="ccpA"
                     /locus_tag="MJF80_000161"
     CDS             complement(22034..23050)
                     /gene="ccpA"
                     /locus_tag="MJF80_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBZ0278847.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(23303..23503)
                     /locus_tag="MJF80_000162"
     CDS             complement(23303..23503)
                     /locus_tag="MJF80_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278848.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(23537..23716)
                     /locus_tag="MJF80_000163"
     CDS             complement(23537..23716)
                     /locus_tag="MJF80_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278849.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(23898..24104)
                     /locus_tag="MJF80_000164"
     CDS             complement(23898..24104)
                     /locus_tag="MJF80_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278850.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(24152..24577)
                     /locus_tag="MJF80_000165"
     CDS             complement(24152..24577)
                     /locus_tag="MJF80_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278851.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            24749..24976
                     /gene="acpP"
                     /locus_tag="MJF80_000166"
     CDS             24749..24976
                     /gene="acpP"
                     /locus_tag="MJF80_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBZ0278852.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(25120..26124)
                     /locus_tag="MJF80_000167"
     CDS             complement(25120..26124)
                     /locus_tag="MJF80_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBZ0278853.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(26138..27040)
                     /locus_tag="MJF80_000168"
     CDS             complement(26138..27040)
                     /locus_tag="MJF80_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBZ0278854.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(27235..28410)
                     /locus_tag="MJF80_000169"
     CDS             complement(27235..28410)
                     /locus_tag="MJF80_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBZ0278855.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(28497..29342)
                     /locus_tag="MJF80_000170"
     CDS             complement(28497..29342)
                     /locus_tag="MJF80_000170"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBZ0278856.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(29440..30237)
                     /locus_tag="MJF80_000171"
     CDS             complement(29440..30237)
                     /locus_tag="MJF80_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBZ0278857.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(30256..31266)
                     /locus_tag="MJF80_000172"
     CDS             complement(30256..31266)
                     /locus_tag="MJF80_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBZ0278858.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(31285..32067)
                     /locus_tag="MJF80_000173"
     CDS             complement(31285..32067)
                     /locus_tag="MJF80_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBZ0278859.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(32126..33262)
                     /locus_tag="MJF80_000174"
     CDS             complement(32126..33262)
                     /locus_tag="MJF80_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBZ0278860.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(33971..34438)
                     /locus_tag="MJF80_000175"
     CDS             complement(33971..34438)
                     /locus_tag="MJF80_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBZ0278861.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(34517..35059)
                     /locus_tag="MJF80_000176"
     CDS             complement(34517..35059)
                     /locus_tag="MJF80_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278862.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(35111..35797)
                     /locus_tag="MJF80_000177"
     CDS             complement(35111..35797)
                     /locus_tag="MJF80_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBZ0278863.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(35797..36657)
                     /locus_tag="MJF80_000178"
     CDS             complement(35797..36657)
                     /locus_tag="MJF80_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0278864.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            36792..37592
                     /locus_tag="MJF80_000179"
     CDS             36792..37592
                     /locus_tag="MJF80_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBZ0278865.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            37617..38186
                     /gene="yfcE"
                     /locus_tag="MJF80_000180"
     CDS             37617..38186
                     /gene="yfcE"
                     /locus_tag="MJF80_000180"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBZ0278866.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(38522..39574)
                     /locus_tag="MJF80_000181"
     CDS             complement(38522..39574)
                     /locus_tag="MJF80_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBZ0278867.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVIEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(39781..41007)
                     /gene="pepT"
                     /locus_tag="MJF80_000182"
     CDS             complement(39781..41007)
                     /gene="pepT"
                     /locus_tag="MJF80_000182"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBZ0278868.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            complement(41098..42150)
                     /gene="ytvI"
                     /locus_tag="MJF80_000183"
     CDS             complement(41098..42150)
                     /gene="ytvI"
                     /locus_tag="MJF80_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBZ0278869.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            complement(42299..43552)
                     /locus_tag="MJF80_000184"
     CDS             complement(42299..43552)
                     /locus_tag="MJF80_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBZ0278870.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            complement(43683..47210)
                     /locus_tag="MJF80_000185"
     CDS             complement(43683..47210)
                     /locus_tag="MJF80_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBZ0278871.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYKKIN
                     KIKNEEIPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            complement(47197..48408)
                     /locus_tag="MJF80_000186"
     CDS             complement(47197..48408)
                     /locus_tag="MJF80_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBZ0278872.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            complement(48454..52281)
                     /gene="addA"
                     /locus_tag="MJF80_000187"
     CDS             complement(48454..52281)
                     /gene="addA"
                     /locus_tag="MJF80_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBZ0278873.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            complement(52281..55748)
                     /gene="addB"
                     /locus_tag="MJF80_000188"
     CDS             complement(52281..55748)
                     /gene="addB"
                     /locus_tag="MJF80_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBZ0278874.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIINFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            complement(55738..56568)
                     /locus_tag="MJF80_000189"
     CDS             complement(55738..56568)
                     /locus_tag="MJF80_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBZ0278875.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            complement(56677..58896)
                     /locus_tag="MJF80_000190"
     CDS             complement(56677..58896)
                     /locus_tag="MJF80_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBZ0278876.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            complement(58889..60802)
                     /locus_tag="MJF80_000191"
     CDS             complement(58889..60802)
                     /locus_tag="MJF80_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278877.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRVEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEIEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQDSILYSI
                     LLKISHKGDKNYD"
     gene            complement(61030..63042)
                     /locus_tag="MJF80_000192"
     CDS             complement(61030..63042)
                     /locus_tag="MJF80_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBZ0278878.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKIYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDNELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            complement(63547..65430)
                     /locus_tag="MJF80_000193"
     CDS             complement(63547..65430)
                     /locus_tag="MJF80_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBZ0278879.1"
                     /translation="MVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTE
                     SYEGTINYNKDPEYLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTV
                     NASQTRLKGSNRYETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKNSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGAD
                     RHETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KDNLEAKSANKVYKIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     DTGTTGKPLGGIKEKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSL
                     RPDLFTSIHYNASDTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKT
                     RTLSTDPKKDYLYVLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIED
                     SLK"
BASE COUNT        20309 a        11478 c         6846 g        26799 t
ORIGIN      
        1 gtaagtctat aaccttctag tttagttcac aaacttacct tgactttatt tttataaatt
       61 acttagttgg aactgcatta tcttctatct cccaaaggtg aaaacaattt tcatgtaaat
      121 ttatatattc ttcttttggt ggtagtattt gtactatggt cttatttggt ttcatgagtt
      181 tgtatctaac atacttaatt tcattccaat taggaaatct tttttcattt gatatagtta
      241 aatgttcttt gccattttct actgaatata taacattact cccagtatcc atgttgtata
      301 tttttacatt tttagcccct aatttttcaa gtgattcttt tgcaagaata gacctatcat
      361 ttttactctc aactaataac attttattcc ctccaaattt tataaataat tttttgtctc
      421 cttctctagc caattttcat atgctatatc acaatcttta ctttcacaat ctctcctatt
      481 atttatacaa ctagcacaaa tctctttccc aaattcctta tacacttctc tttcatcaag
      541 attctttgac ttgcacattt ctttattagt catatgctca ctccttgcat tttctaaatg
      601 attcaaccac catttcagtc tctccacaag tatcttctgt aaaatctatt tgtctcccat
      661 taaattctct tatataatct gctatatcat atactctttg acatttattc tctatacagt
      721 tacaaatatc atttatattt atttcgtttg gaatttcaac tataacttca tgttctaaag
      781 tcactttttc ctcaaactta attttatatt ttttcatttt aaatactcct ttatttcatt
      841 tttgagagtc acaaaacact tcaacaataa tttatactaa aagacatttt gcaactctct
      901 aaactgtttt aattacatat tttccatatc catttcatat ttagataaaa tctctttaaa
      961 tgcttttgct aactctgttc ttataaaagt tgaatttgaa tctttagcag ctatatcaag
     1021 gtctatcttt tgataatcct ctttgttagg aaacttaaat cctattctta taggacaatc
     1081 tacatcctca agattttcta taactctctc tgcaaactct gcactacctt gtaaatcctc
     1141 ataactttct actctatttt ttaaatcttc atattcttta tattccataa tctattcctc
     1201 ctctaaactg ttttaattag atattttatc atttcaaagt tagttatttt tagtaccttc
     1261 ttcatttctc aataattcat gagctttttc ttcatcataa ctgcaaatat ttctaccatc
     1321 acaataatga caaagtaatc tagtattttt agtaacaata ggaaacattt ttttgcaaat
     1381 ctccaatcta tcatccattc ttatacacct cttttgttat cacaattttc acactctttt
     1441 agattcaatc tatactcata aaccctacca gctataaaac ttcctactat aagtattaaa
     1501 ctagctaaga tgttcatttt ctaacatctc cttacgttct aagaactctt ttttaatttc
     1561 ttccaaattc tcgcattcat taccaactac tacatatttt ttgctattca ttatagttgc
     1621 tttacttgta atttcctccc atttaccttc aaattgcttt aaataatgcc attcaacata
     1681 taattcaaga gcataatttt cttgtcttac aattccatat tcatatttat ttttagtatt
     1741 atagggttct ttcaatatat ctccctcata aatttcttca ttatttccag accaacattt
     1801 cgagaatact ccaacattag atactttttg ccattcacaa ttctctttta gcataaataa
     1861 acaatctact gcatcactcc actttattgt ttcagaataa atccacttct cattctcaaa
     1921 atcataacct ctgtacttaa ttagactcat ttctaatcat cccctcgcac tcatatttgc
     1981 ttaatatttt tatagctata tcaatagctt tattaacaga acactttttc ttatttaata
     2041 tcttttcagc taacttaatt acttgctcca catttgctaa taccatctga cacctcttga
     2101 atatattctg ccttccaacc ttctctagtt actctatttt ctctagctaa acaactagca
     2161 taatctccac taatctttaa gtatttactt gcagcctttg cacttttaaa tattctaact
     2221 tctccagttt caatattaaa aacttttata ggtttacttt ttgtatcatt agttggtttt
     2281 ttccttattt ttttgaattt cgattcatta agtttcagct ttattttctt actttcattt
     2341 gcttttctta cttttcttaa attaagctca aacatatctt ctatatcaat agttttttct
     2401 aaaaacgttc ctgcatctaa ccaaatttta gccatatcta attcctccta ccttatttaa
     2461 cttcaacaca ccattccgaa actacaccaa aattaaacca aaattcattt ggatagcaaa
     2521 catagccatc atcaaaagtt ttttcaacag taaattcaac accttcaata tcatcaatcc
     2581 aaagttcttt atatttttta tgttgttcta caccattaat ttccataaat ttctctttat
     2641 caaacttata agttttacct atctcaaatc tcatattgtt agtcctcaca ttttcttaat
     2701 aattcttcta agcaactctt acatataaca attacaaatt ctctaccatg taaatccata
     2761 acctttgtat ttgtaaactg gtcatcataa ctctcaacta gaaactcccc acaaacacta
     2821 catatagctg ttctactcat ttttatccct ccatttttaa cttttaggaa gtaatattgt
     2881 ataattactc cctagactat ttaacttaat taaaaaggta tatcgtcatc atctattgct
     2941 tgaaaacctt gtgggtctaa tcctggtggt acatattctt gtttagcatt attatcattt
     3001 ttactagaaa gtagttctaa agcatttaca ttaaccttag taatagattt ccagcaacca
     3061 ttttcatctt tgtaattata tatatttaac tctccaacag catatatagg cttaccttta
     3121 acaagatatt gcactaaatt ctctacatgt tttcctaatt gctcgcattg aataaaatca
     3181 gttattttat ttccattttt atctttaaac cttctatcta ctgccattga aaaggttatt
     3241 tttggagtac ctgaatttgg aaggtacttc aattctgcat ctgcaactaa tcttccaact
     3301 aaagttattg tattcattta actagccccc ttctattttt cttcctgttc ttctgtatac
     3361 tcaacaaagt aagtataagt tgtcttgcta ttttgcttct ctctagcaac ctttactgta
     3421 tatccagctt tcccaagtaa tcttaataac tccaatctat cttgttcatt taaagaacca
     3481 cttctttgtg catatattct cgccatttta tacctcccct tttctaggaa gcaatatatt
     3541 gatatttact tcctagaagt ttaattttat ttaaatttaa ccttttggct tttcttaatt
     3601 atgtcatcta gttcgtcagg tgaatattga gtaaatgttt cattgaagtt atgaaactta
     3661 tttttactca cattaggagt attcacagct ttactattag actgcttctt atcctgttta
     3721 ttctttttct tcctctcaaa ctcattttga tactctgtaa gttctaaatt agtttttaca
     3781 cctgcttcta tccaattatt taatattgtc tttacatact tataattctt aactccattt
     3841 gctattgctt catcaatagc tcttataatt acatcagctt ccattccatc atctaagtaa
     3901 ctcattaact ctataaagtt attaggagta atcacaccta tatatttttc aaagtatttt
     3961 tttatgtagg tggttttgtc tttattggat tgttcattaa taacaatagt agtaatatca
     4021 ttatttactt taaagtcatt acttactact tccgtgtttt ccggtttccg agaaacccgg
     4081 tttccgggaa atccggtttc cgggaaatca gtttttcggg attttagctt ctgaggattt
     4141 tcaagtggta tctcatatac ttgataatca tatcctccaa gcatcttatt agtattagaa
     4201 tctcgacaag gtgttcttgt tatatatcca ttttctatga gctcccttaa aatatttgct
     4261 gtagcatccc tcccattttt acttctttta tataaatcat taacatagat tttccagtgg
     4321 tcgggcttac taatcagata tgaatgtaaa ccttttgctt gccagcttaa tttcacatct
     4381 tccaaacaag ttttatttaa aactacatat ggattatctt tgtctttgct tactcttata
     4441 atccccacaa tatcacctgc ccttattgct tattttcata agcattacaa atagtgtcat
     4501 actcttgttt tgttaaatct tttatctctt ttccaaatct tttaaatact ttttctttca
     4561 aactttcttt atcaacattt gcattacttg ctattgcata taacctactt aattgtttat
     4621 ctgttaaaat tctattgtta gaattacttt tagcttcatt tttgccacta gttgcgtcaa
     4681 aagtgtcact ttcagttatg ttgagtaact gaatgtacaa atatctagtt tgataggttt
     4741 ctatacctcc tagtgcttgt aattcattag aacctttaag ttgtaaatct ctcatgggag
     4801 aagtaaatac aatctgttct gatgggtttt ccccattaat taatgttaga gttgcatatt
     4861 catttgtaaa ggtcactata gggcatagct tagcttcttc aagtaatcca gttgcttgtg
     4921 gtagaaagtc tgctaactca aaatacttga agttagcgaa cttattttct ccacttttct
     4981 ttagattcaa cttactaaat ttaactctta catccatcaa tttaatgtaa atattattaa
     5041 tttccatggt cctcacctac tcttttttag cttttggaat tgttagtgta gttccatatt
     5101 caatcctgca accttcaacc tcatgacctt ttttaataaa atctttaata gtgttcttat
     5161 ctacttttac aacttgctct actgttttat atatagcagg tatcttttct tcatcttcta
     5221 tgactaagct acctgctgac tttcttatac ttatatttcc taaaactgtt tctacttttt
     5281 tagtcccaag taattccata cagtctttta tattgctttt taatctatca agagtattct
     5341 tcttaacctt ctttaactct tgtaacctct taatctctga atctatagag tttatatcac
     5401 tgtcaatgtt taatattact gacactattc tagtgttttt attttgtatc tcttgtttta
     5461 ttatttcttt tatttcctct agtttttcag cttcatttcc tgttgtttct gttaaacctt
     5521 cttctatttc taataaatct gtagttaatt catataaagt actcataatt tccctccgtt
     5581 tgtgctataa ttagcttata ttttatatta ttttggaatc gagccactcc taatggctct
     5641 tttctatatc tgaatatcta taggtctatc tctttcaatt tcttctgaaa ttaattcaaa
     5701 tatcttgtaa tcctcgcttt cttcatattc ttttatttca atttgtgtat ctataatttt
     5761 tagtaatgac tcagcaaata tttttaatct ttcatttaca cttttttctc ttaaagcatt
     5821 acttaactct atttcatcta atacatctct tttttctttt tttctaagtt ttgtataaag
     5881 ttcctcattt ttatttattt ctaaattagc tctatttagt tgttgctcta ctgcatttct
     5941 tactatagtt aaacttttca tgattaatcc cccttaattt ttaatttact tggtaaatac
     6001 aagttaacta actctatatc tctgttgtat tttctaagac cttcaaaact tgcttttatt
     6061 tgcttatcat tgcaaaactg tacataagct attagtactc ttacattcaa ctaaatcacc
     6121 ccctctctaa tttctttcat ttctctaagc atttctttga tgttttttcc ttgatttcta
     6181 gttataaaat catctaattc ataactagaa actttagttg cccctatatc aactgacttc
     6241 aaaagcccat tttttattaa ctcatatcca aatactttat ctattttcaa tcttttactt
     6301 gcttcttcaa cagacataag ataatcggga taacctttac ttataacaat tgttagttct
     6361 tttggttcta ataattctat tttcgaagtt tcatttaagt actttgagat tttattttta
     6421 tagttgttta aattcatttc tacaacttta cggatacctt ctgaaaaaca aattgatata
     6481 ttatctaggt cattaaaact tttatcttct tgtttatcta aattgaaatt agatatattg
     6541 cccaatttac tcactccttt tcaaaatatt ctgtatttaa tttttatctt ccaactagtt
     6601 catctaatgt aatgtctaaa tagtcggcta tttttattaa tgtatctata gttggatttt
     6661 tattttctcc tcttaaaatt gcatataaat tccctgaatc tacacctatt tcttttgcta
     6721 atttccatgc ttttaaatct ctatctttta aaattttatt tatgttgtca ttaattgcca
     6781 ttattttcct cctttgatat actatatttg taggatatat cctatatctt tttatgaaag
     6841 ttggtgatat tatgcagttc agtaaagata tattacatac tcttacttta gaaattctta
     6901 aggaaaaata tgattttaaa agttcttctg aagaggaact tttaaaacat taccatgaaa
     6961 tctttttgaa actttcagaa gtcaataata gtttttctaa aggcgatggc ctcagtgtct
     7021 ttaaacaaat gtaggtacta aattatattt aagagcttct ttgcaaaaat ctaaaatatc
     7081 ttttgaggaa aggatacttt gttcattttc attcaatgta ttaagtatcc ttttagctat
     7141 ttgtaactct tcttttgata atattaattc cgtttcatta tttacattgt tcactacact
     7201 ttcaaatgaa attttcattt aattacacac tcctttttaa aatattctgt atttagtttt
     7261 caaagtgctg ttatatttta acttaacatt gataattgat ttactaattt caatttgttg
     7321 ataaagtata tttgaccttt gcctgttact tttggtgttt tagtaatctt tgtacttccg
     7381 tcagggttat atattgctct ttttttagtt tccattattt ttaagtcaac actcttttga
     7441 gttggtgtat tgtaatcttc acccttacgt tttattaagt aaccattatc tctaagccat
     7501 gcaaataatc ttttctcgcc tgtatcaatg ccattttgct taagtagttt tgcaagttct
     7561 ccaactaata tagaatcatc cgaggatgct actgcatcag caaacaatac ttttggttgc
     7621 tgtaactgaa ttaccttatc tttttcttct atctctctgc ttttcttttc tattgtcttt
     7681 tgagctactt gtaatgctct tgccattatt tcgtcgtctg tcatatcttc tgttgtatgt
     7741 atatatcctc cagttttacg tattgttggt aaaacttcat caaataccca actttcaaat
     7801 ttttctgcat ttggtagttt actatttaca atcaatctat acatatcact ttcaggtatt
     7861 gtatttactt ctaatatctt attttcattt tgcggatgag gtatgtaact ttttgttacc
     7921 cacctacaat ggtcatttat tgctttactt gtgtttgcat atcctaaaca tttagctata
     7981 tctgttgcaa caaaatatgg tttcttatca acctcaacca ttcttatttg accaaattcc
     8041 aatttttcaa atatctgtag attattcata tttattcctc gctttctaca ttgaatttat
     8101 tttctttttt tattttctca ataaactccc aacatgcatc cacaataata gaatttttac
     8161 tttttccaca ttctttggct atattctgta catattgatt taattttggg acgagcctta
     8221 ctgtcattct tactttttcc attcttattc ctccctcctt atgacactta ttcactgtct
     8281 ataatcatta tattatgaca ctattttagt gtcaagactt ttttaaatat tttttgtata
     8341 atgtcattag ggaggtgtca aaatgactac tattactgta agaatatata caccattaaa
     8401 tgaaaattta gaaaaaattt cttatcaaac aggcattctc aaatcttcgc ttattctata
     8461 cgctattaat gatattatta gaaattcaaa agttaatgaa cttcaatcaa tctcgtataa
     8521 aagtgatgat actgttcgtt ctactcttag gattcctggt gtcctaaaag agttgctaga
     8581 gaaaacagct aaagaaaata atttatcagt caattctcta ataaataatg ctgtgcattc
     8641 attttgcata tctcattggt taatttatct ttgatatata caaccaacac gcaattatta
     8701 caatagatgc aataggcatt ccgatttcct tacttaaatc tatcaacgct ttgtacaagt
     8761 cttctggtat acgaagcgtt gtttgctcgt gtttcaattc aatcacctct tttgaatatt
     8821 ctgtatttag ttttcaaggt actgttatgt tttaacttaa cattgataat tgtttgctac
     8881 ttttaaattt attaatgaag tatatttgtc ccttaccagt aatcttaggt gttttagtaa
     8941 tacttgtatg accatctgga tgtactcttg taccttcttt tgtttctata actcctaaat
     9001 ctacactttt ttgagttggt gtattgtaat cctcaccttt acgttttatt aagtaaccat
     9061 tatttcttaa ccagtcaaat aatctatttt gtcctgtatc aattccattt tgtctaagca
     9121 attttgctaa ttctccaact aggattgaat tgtcagaaga cgctaccgaa tcagcaaaca
     9181 gtacttttgg ttgttgtaac tggattacct tatccttttc ttgattttct aattgtagtt
     9241 gttctttttc ttcaacttcg attaataact gttgcagtgc ttctttatat gtagttggta
     9301 atttaggttg ttgttctttt aactcttgct ccatttcttc aaacttagtt acataaattg
     9361 ctgtaaatat aatccctttt tctcctgtca ttttattagc taccatatcg caaccttttt
     9421 tagttaataa gtagcaaggt tggattttat tttgagtatt tatataagta ctttctataa
     9481 agaaatcttg actcctcaaa tttgatgagt cctctaaaat cttcttgtat cctcttatat
     9541 ctcttaataa attatcgtgc ttcttttcta ttaattctgc tacttctcta ctttcaacta
     9601 aaaattgatt attttgcttg attatggtta gattcttcat tattattgcc cctttcttct
     9661 tttattgcta ttttagcaac ttcatctgaa aaaaaataat ccgcagatac attatataat
     9721 ttagatatct tttttatttc acttgcttta aattcgtttt ttccttttaa ttttaatcta
     9781 aaaccatatg agctaagacc taatatatct gccacatttt tttgggtgtg tctattttcc
     9841 ttcatcaatc cttctaatct atttaagtac attaaatcac ttcctttttg ctattttggc
     9901 aacttcatat ttatataata tcaagttttg tgattatagt caatactttt gttgcttttt
     9961 tagcaaaaaa aattgtaata attaattttg ttgctataat ataaataaaa gttgctattt
    10021 tggaattaat ataaaaaggg gttgtgcaaa ttgaatagga taaaagaatt gagagaagaa
    10081 aaaggcatct cgctagacaa attaagcgag gatttacata taaacaaatc tacactatca
    10141 aggatagaaa atggtttaag agaacctaag aaaagtacaa tagaagaata tgcaaactat
    10201 tttgatgtgt ctacagatta tttattagga agaactgatg ttagaaatag cttatttata
    10261 aataaaaacg aaaaagatta tgatgctgaa aattttaaaa cagaaaagga gcttattgag
    10321 aatatgtatc ttgacgaaga tatgaaagaa gtttttaata tatttagcga gctaagccca
    10381 gacgcaagag aaaaagcatt aaaagttgca gaattatttt tactagacga aaaaaataaa
    10441 aaatagttta tttctgaaaa tgaatataat aaaaaagagg aatcattcct cttttttatt
    10501 gattttatgt atttctttca cttttatttt atattcattg attttatttt tatccaattc
    10561 tttcaacttt ttcattaata agtttaactt taaaatatca taatatttcg tcttattcaa
    10621 atatatcatc ccctataaaa tattttattt attaattcca cgaaacatac gttcttaaaa
    10681 atagttacaa ccacctcttt ttcaaagctt aaaactataa aataactgta aaatattatt
    10741 atattttata gcttctatta tttttgcttt atctagataa agttatttct taactacatt
    10801 ctagcacaaa tttccaacaa aaagtgtgcg aatattgcac atttattaca agaaattaca
    10861 caaactaaca tatataaaat tatctaaaag gtaggttaaa tatatgttaa aagagttacg
    10921 aaaaaagaag aaattaacac aaatagagtt agcaaaaaga gttggttgcc acagaagtca
    10981 aatttctagg ttggaaaata atgaggataa agatttaact atctctattc ttattgaatt
    11041 agaaatagct ttaggattgg aagaaaaata tttagtaaat tattttgctg atgaatatat
    11101 taaaaaaaga aaattacata aataattcga atgtttctat caaatactat ttttaatata
    11161 taatattatt tgaggtgaat aaattgaact taagttttaa taaaaagaaa aaatttgaat
    11221 taagcaaaga agaattggaa ttaattgaaa attggttagg taataaatat gcaggagata
    11281 tgacgatacc tgctattgtt gacttttctt tagaaactga tattgagtat gaaaaaatag
    11341 ttgtttattt ggcagaaaaa gtgctggaat cacgtgataa aaaacattaa atatgtatct
    11401 aaataaaaaa gactattaat tataaacata aaactttgtt tcttatagtc ttttttattt
    11461 attttattta aattgatata tgttattata taattacata aaaaagcttt tgagaggaga
    11521 aattttatga aaggcggcgt aagaaaacgt ggaaagaagt ggtattacta ctttgatgca
    11581 ggtatagtag atggcaagag aaaaaaggta gaaagagttg gtggaaacac taagaaagaa
    11641 gctgaaaaat cgctccgtga tgcaataaat gaatatgaaa acgctggtat agtgtttgat
    11701 gaaacaaata tgagtttatc agactatctt aacttttggt acaaagagta tgtacttctt
    11761 aattgcaaat acaatactca ggaaagttat agaaatttaa ttgaaaatca tatagaacct
    11821 agacttggta aatgtaagct aaaatctata aatccagcta ttattcaaga atttttaaat
    11881 aataaatcaa aagagacata cacacaaaac ggagaagaaa aacactacac aaaaggagtt
    11941 ttaaaagcga tttatgttgt attaaatgct gctttaaaat ctgctgttta cccttacaaa
    12001 ctcattaagg aaaatcctgt tcaatatgcc agtataccaa aaaatgtttt aaaggtaaaa
    12061 aatgagtcag ataacaagac tataacacta gatgagttca ataaaatact agaaatatat
    12121 cctaaaaata caaatatcta tattcctcta cttatagggt ttcatacagg catgagaaaa
    12181 ggagaaatat taggtctttg ttgggataat gttgatttag ataataatat aatcaaagtt
    12241 agaaaaaatt taataaagag aaaagtttca gaatttgaat tagcatcacc taagacaaaa
    12301 acatcaataa gagatattaa aataggtgat actttgtcta gaatattaaa agaggaaaaa
    12361 ttgaatcaaa aaaaacaaaa aattaaaatt ggaaaatggt ataaagaaac tgagtatgat
    12421 tgggtttgta gaaaaaaaga tggctcattt gtaaatcata acaacattga tgcttgtgtg
    12481 agaacaatta acaaaaaatt aaaccttgac ttcaacttcc attgtttaag acatacacat
    12541 gctacattat tattagaaaa tggagctaat ataaaatata tacaacaaag attaggacat
    12601 agtcaattat caactactat ggatacatat tcacatgtta caagtaaaat ggaaagtgaa
    12661 acagtagata ttttggaggg tattttacaa taatttgcca ccgaaaaaaa attatggtgg
    12721 caaacaggtg gcaaaacgat aaaaaacatt cttttttttg ctcaaacact gttattttgc
    12781 tattttatat aaaaaagtcg tatttctcac agttatacga cttatattaa attctttaca
    12841 taagtctcct tcagatggaa gtttatctcc tggtttatat atgccagatg ctatttgttt
    12901 ttttatgttg tcatatagtt gttgatataa aggaacaaag gaatttactt ctaaatccat
    12961 ttcttttttt tgtctctttt catccatttt gctcacctcc ctatgtaaca tttttataac
    13021 attatataac aagttcttta ttacatatta taacaagatg aaatcgttgt caattttttt
    13081 taaaataatt tttaatttga cagtattttc ttttattttc tctagtgtga caaattgcta
    13141 tttttacata aaaataagac tgttgtacaa cagattaaat tgtttataca acagccttta
    13201 ttcacttatt acatttatat ttttacttat aaatttatat taaaaaatgc cttatatccc
    13261 ttatacattt catatatgtc tactttagag acaatctcat gtggtgcatg catatttaaa
    13321 actccaacac cacagtctat aacctctgca ccttgatttg ccaatatatg ggctatagtt
    13381 ccgcctcctc cttggtctac ttttccaagt tcagcagttt gccatactac atttccttta
    13441 ttaaaaattc tctttacttc tgacataaat tcagcattag catcattaca tccactcttt
    13501 cctcttgaac ctgtatactt agttaaaact actccatttc ccatatatgc cgaatttctc
    13561 ttttcatatg cttctccaaa atttgggtca tatccagcag aaacatcagc tgataatacc
    13621 ttactatttg ctaaagctct tcttacttta atatcacaat aatctccttc taaagcaatc
    13681 agttcagcca cagtattttc aaaaaacttt gagtgcatac ctgtattccc tttagaacca
    13741 atttcttctt tatctacaca taatgcaact gaagtaaatt ctggttgttc agtttctagt
    13801 atagctttta cagcagcata tgaacatacc ctatcatcat gcccatatcc caacaccata
    13861 gacctatcaa gccctaaatc tctagcctta ccagctggaa ctacttcaat ttcagcactt
    13921 aataaatctt cttcaactat attgtatttc tcatttaaaa tctttaatat atttgctgtt
    13981 ataggttctt tttcttcatc ttctaaaggc atattcccaa ttaatatatt taaagcttct
    14041 ccactaattc cttctgatag tttcttttgc atttggtctc cagccaaatg aatcaataaa
    14101 tcagttacac aaaatactgg gtcactatct tcttccccaa tagatatgtc cacttttgtt
    14161 ccatcattta atataactat accatgtaaa gcaagtggaa tagccgtcca ttggtatttt
    14221 tttattccac cataataatg agtcttaaaa aaacctagat tactctcttg atacagtgga
    14281 tttggtttta aatctaatct tggcgaatct atatggcttc ctataatctt catacctttt
    14341 tctatataat ttttacctat tacaaaaagt gctactgcct tctctttatt tataacataa
    14401 attttatcac cacaacttat gcttcctttt tccatagctt catctattga aatatagtta
    14461 ttttctttag ccaattttat aatttctttt actgaaagtc tttcagtctt tgacttactt
    14521 aaaaactcca tataattgct agaatattgc ataatttgtt caatagtatt ttcttttctt
    14581 tcaaaatccc aagcattctt aaattcgtac tttaagttca tattttcctc ctactaagtt
    14641 ctaattttta ttgtttttca tgtattgctc atattcttct ggtgaaacta ctttgacact
    14701 atctagtctt gatctaaagt gggctctaaa gttttctaaa tattctttcc ttaaaatttc
    14761 tctttctttg atttcatcag cagaaaggat tccctcttta tttttctttg ctaattcatt
    14821 aatcctatct aatttcttct tgtcaaacat tttttcctcc taaaaaacct ctatttattt
    14881 aataaaataa tttattgctt tttatatcta acttgttttt atattcacaa aaaaactcat
    14941 ctatattatc acttattata gatattccct tttctagttc ttccatgctt ggacgagcta
    15001 tatttatcct aaagaatcta tcatccacta gattgtcaaa aaaataagtc cctggaagca
    15061 ttgacactcc tttgtttaac ataaaatttg taaatgcttg agatgtatat cctcttggta
    15121 attctaaaaa aaagtttatt cctccacaag attttctaac ttttaattta ccactcaatt
    15181 tgtcattaat caagtttttt gccaatttat acttatcagt atatatcttc tctatatgac
    15241 tcaaataatt cttccaatca aaattattca tatagtaata cattgatttt tgaattaaac
    15301 ctggtgttga tatatctgat gaatatttag cccataaaac tttttttaac aactcatttg
    15361 gcatttctac aattcctatc ctaagacctg gcataagtat ttttgaaaag ctttttatat
    15421 atactactct atttttatca tcataacttc taattgttct attgtccttt gattcaaaaa
    15481 caaaatcact tataaaatca tcttctatta tataaaaatc atgttcttct gccaattcta
    15541 tcaatttctt ttttttatat gttgagtaag atattccagt tggattttga aaattaggca
    15601 taatgtatat aagtcttggt tttatttttt ccaactttaa ttttaaaata cctatatcaa
    15661 ttccatcatc taacataggt atagatatta tttttgcacc tctactttta aacacttcaa
    15721 ttgctccatt gtaagaaggt tcttccataa aaacaacatc tgaataactt ataagtcctt
    15781 tacaaactat atctatacct tgttgagcac ctgatattat ttgaatattt tccttagtag
    15841 tttttatatt tccatcactt agatactcta ccattttttc tcttaattct tcagagccaa
    15901 gaccttcatc atattcaaat attgaactac catctttaga taatgccata tttacagcat
    15961 ttttaaatga atcgatagga aacatatcat tagatggatt cccattgtca aaatgaatta
    16021 aatcttctct ttgcacaata atcttatttt tcctgttatc tataccgctt ttataagaat
    16081 ttaaacttga tacaaaagta ccactcccta taattttata tacatatcct tcattttcta
    16141 aaagctcgta taccttaact atagtagtgt tatttacttg taaaaaattt gatagttttc
    16201 ttattggagg caatttttca tgttctacta actctttatt tattatcaat tcttttatat
    16261 gattaaatat ctgtatatat ttagttgatt ttttctcatt taaatctatt ctgtattttt
    16321 ccaaatttat caccgctcat tgtatttata ttttattgat gcttttatgt atatttgata
    16381 ttatttagta tgtatatata ataccaaaat ttttataaaa aggggaaata tttatgtttt
    16441 acgactatca catgcactca agtttctcta cagacggaaa atctacaatg gaagaaatgg
    16501 taaaaaaatc tattgaactc ggtttggagg aaatttgttt cacagaccat gttgactatg
    16561 atgtatatgc tgatgattct ttttcaattg tctacgaaga ttattttaag agtttagaga
    16621 ctcttcaaag caaatataaa gataaaatat caataaaaaa aggaattgaa tttggagttc
    16681 aaactcaatt aatggataca tacaaaaagg aagcacatca atatccattg gactttataa
    16741 tatgttctat ccatgctata gatacaatgg acttatacct tggtaattat tttaaagata
    16801 aaactcaaca tgaagtttat gaaaattact atctttattt atataatatt gttaagaatt
    16861 ataaagatta ctctgtctta ggtcatttag atttgattaa aaggtatgca ccttatgaca
    16921 ctattttaga tgatagacta ttttctgata taattgaaga aactcttaaa caagctattt
    16981 atgatggcaa aggtatcgag ataaatacct cttgttatag atataatctt ccagacctaa
    17041 ccccatctaa gtatatactt caaatgtaca aagacttggg aggggaaatc ataaccacag
    17101 gttcagattc acatcatata agtcaagttg catgtgagtt tgactacata tattcattac
    17161 taaaaaatat gggatttaaa tatgtatcta aatttaataa attgaaacca gaatttataa
    17221 aattataaat ttttacacct ccttgtgaat atatttaaaa gataaggagg tgattttttt
    17281 gaaagtacct tatttagtac tatcaacagt tattttattt atatccaatt ttattgtaag
    17341 aatatttggt tttttatata aaatattctt atcaagagca ctaggtgaaa caggtttagg
    17401 tatctatcat atgattttta actttttgat gatttgctta gctgttacaa caacaggtat
    17461 acctactgca cttagttgtc tagttgcaaa gagaaaagct ttaaacgata gacacaatac
    17521 taatgcttta tttatatcaa ctttatatat atcatttttc gtagcactaa ttatatctat
    17581 agttgcatct ttcaatagtt cttttttatc acttaaattt ttaaaagatg ctaaattaaa
    17641 tctatttata ctagctgttt gtcctgctat agttataata actctttcaa acgtactaag
    17701 aggatattac tatggcataa agaatgtaaa aatccctgct attggacaaa tcattgaaca
    17761 aattggtaaa atattatttg tttttttact tgttatgtac ataaataata aatctatgaa
    17821 ttgttatata gctctattag gtatatcgat tggtgagttg agtaatatta tatttatgct
    17881 tatttgctta tggcgagatt catcatttga caataggtat attataagca taaaagattt
    17941 ttataattct tctatggaaa cattaaaaat gtctattccc ataacttgta atagaatgtc
    18001 aaacatactt ttgcaatcta taagttctat gatgatacca tcaagattag cattatcagg
    18061 aatgacttat caacaatctt taagtatgta tggtattgtc agtgggatgg taatgccttt
    18121 tatattttta ccttttactg ttggttcagc gcttattgta aatttaatac caactatttc
    18181 acaagaaatg gctctaagaa agcgtaagag tgtaattaaa aaaatcaaat actccatact
    18241 tcttacttta tttgtaggca tactatcatc aatatttttt tatttctttg gaaaagattt
    18301 atgtatatta gtatttaaaa atacacttgc aggagaatat ctaaaagcta tgtttttagt
    18361 tccactattt atgtccctaa atcaaactct atctggcatt ttacattcaa taagaaaaga
    18421 attagcttca agtataaata ctattactgg catgttaatt caactaatag ctttatatgt
    18481 atttcttcca attccaggac ttaatattta tgcatacata tacacaatga cgatagtatc
    18541 aatctttact tgtctattac atactatagt gctttttaaa tcactaaaat ctatacgcta
    18601 gttataaaaa cgtgaaggct accaatgtaa attatacact ttggtagcct tccttagatg
    18661 atttttattt ataatttaaa ctgtctatat atctacctat ctatctgtgg caacttggct
    18721 ttccacttcc atgattaggt ccacattgcg aaaaatcaca tactttagag aaagtatcaa
    18781 caagtcttgg ttggattgga tttggtgatg ctaaaactac tatttgttca cttattaatg
    18841 tgctaactgt ttcttgtaag caaagtacta aatctatatc atttggtata attaattcat
    18901 agaaaggttc acaacattcg ttgcattcta attttaatat agattttcct atacaatcta
    18961 cagttaagtt agtttgatat tcttgtctta aagtaacagc tcttccgtca gctggcaagc
    19021 atattggtgc tgaaagtgtg ttaaaagtaa attctacata atcaactctt ctcttacatc
    19081 cacctcctgc tctaactgct gggaaagcta atgcaacgaa ctctgtacat ccacatctac
    19141 ctcttagctc tagtactaaa ttacgaactg ctacagttaa tcctctttgt ttataagcta
    19201 ctgatttgcc ttttccttgt ctacagcact cagagtttct ttctccacaa actgcaaatt
    19261 caaaagttgt ttcacagttt cttcctacta tatctcctaa tgggtctaaa tcgaaatctt
    19321 ctaaagttgt cattcctgtg tctataacga ttccatcatt acttaagcat attttttcta
    19381 tagttacact tgcttgacct gcacttggaa ctgaaccaaa tacttctact acttctgttt
    19441 caaaagttaa tggctctcca tttggtcctg ttgcatctgt aaatgtttgg aattgcatag
    19501 tatcaaatat tctattagcc tctattgcaa atggcactgc atttgataca gagaagtttc
    19561 ttggagatat aggattacag cattctggtc cacatttgtt ttcaaaacat tcatctgaat
    19621 ccatttcaca tggttcacaa tcacatttac aattgtcatg gcatccacaa ttgtcatcac
    19681 atggattagg tttgcatgga ttgcatgggt taggtttaca tggattacat gggttgcatg
    19741 gattgcaatc atcatggcac tcacacttat tatcagtatt acatccatta tgatggtgtg
    19801 attttttaaa acctctcatg tcttctgaat ttatttcatc tgtatacact tcttcttcat
    19861 tcattgtaga cattggctga ttcatcattc ttcttttatt ttttttataa tcttgcattt
    19921 ttcattgccc tccctgtaag cgattcttat taagtctttg gctttttgaa gcctaattaa
    19981 tactatgctt ttataatctt aattgttact tgctttttga atttatcaat aattaggcgt
    20041 atttagtaga aaaatgcata ttaataaaaa aagtactact atttgaaata gtagcacttt
    20101 ttttgtcccg aaacaccggt ggctaagtaa attcacaccc taggcactcc caggtgggtg
    20161 acttgccagt aacctttaac gtccaaacta actaggcatg ttataattta ccagacacgg
    20221 tctcccgtat ttcatttgta ggttgaattt aaaaaagcct tatcgtatgt ttcaggattt
    20281 catattatta ttatataata aattttgaat tttttaaagt ggttttatat agtaattgag
    20341 ataatcttat tctatcatta ctttttcttc cttttttctt tatgtattta gttaattatc
    20401 gttttttctt agctctagtt ttagtatttt tttcattata attgtattcg gattcttgct
    20461 cattaatgtt gtatagttcc tcatcgtata ctatatcttc atcattttct atattttcat
    20521 agcctgtgtt cttatcttct actaattcat cttcattatc ctccatttca ccttcatctg
    20581 ttaactcatc atcatccaat tctttgtctt cacaatctaa ttcttcgtct tcataattta
    20641 actcatcatt attgtcttca tcatcattat tactatcatc acctcttaaa agacttccta
    20701 acaatttgag attattcata ctaccaagat tgtttatatt tgccaaagag ctcaaactaa
    20761 atccaccttc accaaagtca tctggtaaaa ataattctgc aaataaatct atcttctttt
    20821 tcattcctat agctttttca actgaatttt ttcttttttc tgggaataca tctataattt
    20881 cctttatcat catattcttt ttatcatcac tatccatttt tctgaaaaaa tccgtttcat
    20941 cttctgtatc taccatatct atattattta atttttttat tcctctaact aaatctaaaa
    21001 cactttctat ttttattagt atttttcttt catctcttga catgtatttt ttagttcttg
    21061 ttattatttc cattcccatt tctaaatctt cttcacttaa atccatgagc cttaaacctc
    21121 tttttatatc atttgcatca aaccttaaat tgccatgtgt agactgtgaa agtttatcat
    21181 ttgcattttt tttgtttttt ctaactctat tatttccttt tgatttattt ttatcatcaa
    21241 atatagagat attaccatta ctcaatgcaa tcactccaat caaaaatagt aagttattca
    21301 agacaattcc cccttatatt atataattgc cctatattaa agtatgtttt tcatatttta
    21361 atgttccatt tttaaagctc agtgcatata tattatctat aagaattttt atttatatta
    21421 tatatcactc acaattcacc acttcaatat tatttctata tataaatagt atgaatataa
    21481 ttcaatatat atttatagaa aggggttttt ttatgaaaaa attagataca gagtcattag
    21541 aaaaaatggc taaacaaaaa aatattgaca aggataaaat agaaaagatg gctgatagtt
    21601 acaaaggaaa atcagaaaat gaacttatgg aggaacttat aaaaataggt aagaatctag
    21661 atggcagaga tgaagtagta tcaaaattca aagcattttt agatgaaaat caacgcaaaa
    21721 aactggataa tataatggaa aaaatctcag atgcagaaaa ccaaagagat actaaaccag
    21781 ctaaaactaa gaaggctaaa cctactaaag gtaagtcaaa ttcacctgca ccaaaaaata
    21841 caccaggtag tcaacaaaaa aaatctaaaa gtttatttaa gaaaactaaa aagtctaatc
    21901 ctcatgaata gataaaaata aactatttaa gcaattagat aattattttt agttaatcta
    21961 ttattaaatt tgatattatt gacataaaaa atcaggtagt gcctaaggca ctacctgatt
    22021 gattttaata gttttatttt tttctttctg taacactttc tctatcaaca attctataag
    22081 gtaactctat tttcttattt tcaattaatt ccttatttat taattttata accattctta
    22141 ttgctactgc tcccatatcg tatagaggtt gatatactgt agtaagttta ggtctataca
    22201 ttctagctat cttaacatca ttaaatcctg caactgatat gtcttctgga accttatatc
    22261 cagcatcaca tatagcattt atagcaccta tggcagcctc atcacctgtt acaaaagccg
    22321 catgaggtat tattccatca tctaatagtt ctttcatact attgtatcca ctctcataat
    22381 cagttccacc atacttaatc aaactcttgt ctaattctat attgttattt gaaagtgctt
    22441 tctcataacc agcaagtctt tccatttcta aaacagtatc atctttacta gtcattataa
    22501 aagctatctt ttcatgacct ttctttataa gatgctctgt catatcaaaa gtagcttctt
    22561 cgttgctagt acttactgta tatatatcat aatttctagc agttttactt atatatgtcg
    22621 ctggtattcc acaattttgt atatagttga tatgttcttg ttcaactatc catgaaatca
    22681 taactatacc ttccacttgt tttgctctca atagatttat actcttaagt tcttgttcct
    22741 tatcagagta tgtattcgct aaaagaatat catagtcata cattttagca acctcttcta
    22801 tcccatttaa cacctcatta acaaaagaat ctgaaacttc tggaactatt acccctatta
    22861 attgactctt ctttgttact aagcttctag caagtggatt tggtatatat ccagtctctt
    22921 ttataacctc taaaactttt tgtttgactt catcagttac aggttttgaa tcatttataa
    22981 ctctagatac agtagatatt gacactcctg cttgtttagc aacatctttt atcgttatat
    23041 tgcctttcat cttcatcctc cttattttac taaatttatc taaaactgaa ccaaattttt
    23101 gaaggttcct attttaacta catttatata agtttttatt ttaccataaa agcaagtaca
    23161 tttaaataca ttatatttta tattataccc tatattcaac aaatttaata atattcattc
    23221 ttaatttaaa tcatattaaa aaataaaata aaaaaatgct ggtataaaat accagcattt
    23281 ttttattcta atgatttaag tattagcagc cgcaacctac ttcaccatca aagaatccac
    23341 cacatgaaca agttattagc aataatatta agaaaggtat ccatgacatt atgtcaatgt
    23401 ctctccagca atcttcaaaa gctaagaata ggaagaatag tactattatc caccatcctc
    23461 ctccaccaaa gcatccttct tctccaaaaa atctatctaa cataacaaag ccctcctttt
    23521 tttaataact gtgttcttat tctaagcatc caccacagca gcaaagtatt aataaaacta
    23581 ttaggaatgg tatccatgcc attatgtcaa attctgccca gctatctcca aaagctaaga
    23641 ataggaagaa tagtactatt atccaccatc ctcctccacc aaaacatcct tcttctccaa
    23701 agaatctatc taacattata cgttacctcc tgttgatatt ttgttgagct tttcgctcct
    23761 aacatatact atgcatttgg tttccatttt gctaatgtat ttttactatt tttttaaatt
    23821 ttttaatttg aactaaaaaa ggtaaaacca taacttgtat aagttactgg ttttacctat
    23881 ttaataatca atatttttta gcaacaacaa ccatcatcga agttaaataa taacaatact
    23941 attaatataa cccatatcca tatattgtca tcgcataata agtcttctaa aatgtctgtg
    24001 cataatagta agaatactat aactattatt atcaacattt tattcccaaa aaatccttcg
    24061 catcctccaa agaaacgttc catattgtct tctaattttg tcataactaa tcctccttga
    24121 atcattaaga gactaagttt tgtctcttta attaaatata tgtagtaata gacagataag
    24181 ttacttctaa aaagttttta ttgattagtt tatttatttc tttttcacca agtttattgt
    24241 atctatcaac ttcatccaga taatcttttt tctcctctga tatagtatag attaaacttt
    24301 ctattgattt tattttgtac tccttatatc tttctatcaa gaaggatgtt tttgaatttt
    24361 ctattccttt ctttacatgc attaaccttg atatgtaatc ttctttttca ttagatacat
    24421 aactctcctt ttcagctaca tcttcaagga ttaaattggt ctccattaca taatcctcta
    24481 tacgttgcat atctaaagtt attgaattat aataaaaatt tactgctata agtaaaaaca
    24541 ttcctactga agttagtaat agtcctacat atttcatagc atttcctcct aagttatatt
    24601 tgttatcaat aaagtttatt catcaaataa tactttatta ctacatttat aaaattttat
    24661 tattttttat agattccata ttgaaaaatt cttgatatta tattaaacta acattaaaat
    24721 taattaaaac tgctttagga ggcttaaaat gtttgagaaa ataaaagaaa taatagcaga
    24781 acaattaggg gtagacaatt tagatgagat aactatggaa gcatctttaa tggacgactt
    24841 agaagctgat tcattagatg ctgttgaaat tataatggcc cttgaagatg aatttggaat
    24901 agaaatacca gacgaggaag ctgaaaactt taaatgtatt ggtgatatat gtaaatatat
    24961 cgaagaaaac aaatagtatc gttttgtcta caaatataaa tagtctgtgt aaacttctac
    25021 ataataaatt atattagagt tcgcacagac tagattacat ttatttacaa tatataaaat
    25081 acacgctctt atatttaatt tatatactaa gtatataaat tacttttctt cataaaacgt
    25141 aacattcttt attattatat ctatagttcc atcttgattt tgttttacag tatacttcat
    25201 tgggtcttca aaatcagtta gattcccttt tatgtcaaaa ccattatccg tttttatact
    25261 tcttttttta agctttttct caacccattt tttatctata ctaaaacttt cgtcaagacc
    25321 tttttcttcc atatgctctt taaagctatc ctttaactca tcatctttaa ttgaattatc
    25381 aacaaagtca tttatatcaa cttcatgttt ttccttcagt gtataattta atatacttct
    25441 tacatcttca gcttgcttta tatcattact aagagcattg gttatccaat tttcagctgt
    25501 atttttaaat accttggtct tgtacttatc atcatctatt tttttagcat ttaaaaattc
    25561 tgttacaaac ttagattctg acccttcttt ttcagcatcc ttatctaaaa gccttagatg
    25621 aaactcatca tttataccac ttagactaat caaagctcct tgcttttgtc ttaaagtctc
    25681 tgggatacca atttcatttg aaaccatttg aatattaaat ttatcatcca caaattcaat
    25741 tgaatgagtg tacaactttt tataatctaa tttcagtatt gctacactct tttcatcttt
    25801 aatagtgtat aaacaaattg ccaaatcaca agattctagt gcagcattaa ttttcataac
    25861 gtcaaacaag taagatgcaa tttctttaga atttttcaaa aaagtacttt catcatatat
    25921 tatttgttca caacagtttt taattaaatt gtcattataa tctttaaaaa caccttttct
    25981 taaatcctca tcttttgcaa ttctttttat agctttctgg aaaaaaccat ccacttcttg
    26041 attaactttg ccttcaaagt catttaatat tggaacatca ctatttttgt ccaaaacatg
    26101 tattataaat ttatgtatta tcatatatat caccccatta tcttaatttc gatattaact
    26161 ctttcatatc ctctggtata tctgctttaa atttcaattt ttcttttgtt cttggttgtt
    26221 caaactctag cttatatgca tgaagtgctt gcctatttat tagctcttca tctacataac
    26281 catacaactc atcacctatt attccatgac ctatatgagc catatgtact cttatttgat
    26341 gtgttctacc agtttctaag cttacttcta atacagttgc attttctagt ctttctatta
    26401 ctttatagtg agtaacagat gactgtcctc tttcatctat tatcctcttt attgaatctt
    26461 ctgtaggtct ataaattggc tcatctatag ttccctcatt ttcttttata attccatcaa
    26521 ctactgttat atactttttt tcaactttat tttcactcat tgctgtagac aaagtatgat
    26581 gtgcataagc attttttgct actataacta accctgaagt attcatatct aacctattta
    26641 caaatcttat ttttacgttt tctttttgat tatctatata gtaactaata ccatttgcta
    26701 ttgttttatc atagtggctt tttgttgggt gaactaccat aaacggaggt ttattcacca
    26761 taattatatc aaaatcatca taaattatct gtaaatttaa atcttgaggt tcaaaatttg
    26821 ctttttcctc gtctattttt acttctatta aatcaccact atacaccttt aaacttggtt
    26881 ttttatatac tccatttact aaaacactct tttccctctt catttttgat aatgacctaa
    26941 ctgagaaatt tagcttgtcc aataaaactt cttttaaagt catttcttct tcatttgtat
    27001 acgatataag attatatctt tgattctctt ttttaaacaa acttttacct actttctttt
    27061 atatcttcct actttttata tcttcaaaat catgatatta tatattttac actatttttg
    27121 tttaatagat aataattatt tttaactata aaaacctgta agaataacaa atagttattc
    27181 ttacaggtta aattcataat taaatattta atttgtaaat attataatct aaaactatct
    27241 cttaactatt aaagtagttc ccattcctcc acctatacaa agtgtagcaa gaccagtttt
    27301 agcatctctt ctcttcattt catataaaag tgtagtaagt attcttgctc ctgagcatcc
    27361 tattggatgt cctatagcta ttgctccacc attaacatta actttattca tatctatatt
    27421 taagtctctt attacagcta cagattgggc agcaaatgcc tcattagctt caactaaatc
    27481 tatatcttca atagtcatat tagcagcttc taaagctttt ttagttgctg gaactggtcc
    27541 atatcccatt attttagggt caacaccagc tgttccataa gaaactatag ttgcaagagg
    27601 ctctattcct agttcttcag ctttttcttt agccattact actagcatag cagcaccatc
    27661 atttattcct gatgcattac cagcagtaac tgttccatct tttttaaatg caggtcttaa
    27721 cttagcaagt ttctccattg tagtgccagg cttaatatat tcatctttat ctactacagt
    27781 gtcacctttt cttcctttta taacaacagg aactatttct tcatcaaatt ttccttcagc
    27841 ttgagctttt tcagctttat tttgacttgc aagagctaat tcatcttgtt cttctctagt
    27901 tatattccat tgctctgcta tgttttcagc agtaataccc atgtgatagt tattaaatat
    27961 gtctgataat ccatctttta tcattgaatc aacaaaagca gcatcaccca ttcttgcacc
    28021 atatctcgca cttggtacta aataaggaga catactcatg ttttcagctc caccaactaa
    28081 cattatatca gcatcaccta atgctataag ttgagatgcc attgaaacag atcttaatcc
    28141 agaaccacaa actatattta tagtcatagc tggtttttct actggtattc ctgctcctaa
    28201 tgctatttgt cttgctatat tttgtccaag acctgctgta agtactcccc ctaaaagaga
    28261 ttcatctatc atatctggag ttatgttagc tctttttata gcttctttag ctgctgttac
    28321 ccctaactct accgctgaaa ctgatttaaa tgctcctcca aaacttccta ctgctgttct
    28381 agctgcactg gcaattacta cttctctcat attcattacc cccattaaat tagatttttc
    28441 atatatccga aattgttttt taaacaattt tatttaaact tttaattccc aaattcttat
    28501 ttattataat catagaatcc tatcttagtt tttcttccta attgattagc tctaaccatt
    28561 ttagctaaaa gtggatgagg tctatattta gtatctccaa actcagtata taaaacgttc
    28621 attatagcta aaacaacatc taatccaatt aaatcaccta atgctagtgg tcccattgga
    28681 tggtttgctc ctaatttcat agcttcatct atttcttctt tacttgcaac accatctgca
    28741 tatataccaa cagcttcatt tatcataggt ataagtattc tatttactac aaatccagga
    28801 gattcagata catctactgg tactttattg atactcttag ataattcaaa tactgtatca
    28861 aaagtaactt ttgatgttaa ctgaccactt ataacttcaa ctaatttcat cataggaact
    28921 ggattaaaga aatgcattcc tataacttta tctggtctct tagtagaaga agctatttct
    28981 gttatagata atgatgaagt atttgttgcc aagatagtat cttctttaca taattcatct
    29041 agtaacttga aaacatcttt ctttatattc atgtcttcta cagatgcttc tattattaaa
    29101 tccatatctt ttaaatcttc atagttagta gttgaactaa catgacttaa tatttctgct
    29161 tttgtagctt catccatttt tcccttagta actaacttag ttaaattttt atctaataaa
    29221 gctaaacatt tatctatagc accttgagtt ctactcttta aacatacatc atgtccacaa
    29281 cttgcaaaag tttgtacaat accacttccc atagttccac tacctattac agctaatttc
    29341 atagttataa cctcctgtta ttttagtatt tatattctgt taactagatt aaaatactat
    29401 ttaatctagt taacagcaaa tatattttta attagatttt taccctttta taaagttagc
    29461 ttctctcttt tcaacgaaag ctgacattcc ttctttttgg tctttagttg aaaaacaaag
    29521 accaaataaa ttagattcta tatctattcc agtatttata tcagtttgag caccaagttg
    29581 tattgcttct ttagagtatc taactgcaag ctgagcattt ttagctatta tcttagctaa
    29641 tttctcaact tcttctatta aaatgtctgg ctcaacgact ctatttacta gccctatttt
    29701 ttcagcttca tcagctttta taacttgacc tgtaaagatt aattcttttg cttttgccat
    29761 tccaaccaat cttgtaagcc tttgagttcc tccatatcct ggagttattc caagagttac
    29821 ttctggctga ccaaatttag ctttagcaga tgcaattctt atatcacatg ccattgcaag
    29881 ttcacatcct ccacctaaag caaatccgtt tacagcagct atcactactt ttttactatt
    29941 ttctatttct ccaaaagctt ttgctcctaa gatactaaaa tctttagcag ctacagcatc
    30001 taaatctttc atgtatgcaa tatctgctcc agctacaaat gcctttcctt cccctgtcaa
    30061 tattacaaca tcaatagttt catcattatt aatatctaca aatacttcat aaagttcttc
    30121 taaagtcttt gaatttattg cattaagggc tttaggtcta ttcattttca ctgtacatat
    30181 atttccatct acttcaacag ctacattctc ataaacttta acatcacttg tactcataat
    30241 accctcccca agaaattagt tagctaaaac ttcacctttt tcttttgcaa cacttaactg
    30301 actgataagt tctggaagca ctttatgaac atctccaact ataccaacat cagcatattt
    30361 aaatattgga gcttctggat ttttatttat agcaactata aactcagcat cttccatacc
    30421 agctatatgt tgtattgctc cagatatacc acatgctata taaaggtctg gtcttacagt
    30481 tttaccagtt tgaccaactt gtcttgcttt atctaaccaa cctgcatcta tagtggcacg
    30541 agaaccagaa acttctccac ctataatttc agctaattca taaagtatgt ctaagttttc
    30601 ttttccaccc attccacgtc cagcagaaac taatatctta gcatcttcta ttttaacttg
    30661 ttttttagct tcttttatta cttgtacaac ttgaactaat ttatcagcat cattaaattc
    30721 taccttgaaa cggttaatta cagcttcttt agtttcatca ggttcatttt tcttcataac
    30781 ccctggtcta actgtagaca tttgaggtct gaaatcttta caaactattg ttgccattat
    30841 atttccacca aaggcaggtc ttgtcattaa taataatttt gtatcttcag ctactgcaag
    30901 acctgtacag tcagcagtaa gacctgtatg tattctagca gaaactctag gagctaaatc
    30961 tctacctatt gaagttgcac caaataatac aactataggg tcagctgctt ttattgcttc
    31021 ataagctgct tttgtatatg gttcagttgt atacactgct aaagcttcat catctactac
    31081 tattacctca tctgcaccat agtgtgctaa tgtatctatt aaaccttcta ccttactacc
    31141 taaaagtaat gcagaaactt ttgtatcata atcttttgct atttctgtag cctttcctag
    31201 taattctaaa gaaacagttt gaattacatt ttctctttgt tctattacta ctaaaacgtt
    31261 acccattctt tatcccccct taaattatat gatatacttc tcttttaatt tatctatgat
    31321 aattccagct gatgttttcg catcttcatt gtatattgta ccagctggtt taactgattt
    31381 tgtaaatgat ttaaatacac tagttggaga acctttaaga cctaaattag aagggtcaac
    31441 ttctatatct tttacagtcc atgtttctac tacatcattt tcgaaagcat catatattct
    31501 tccaactttc atgtatcttg gtgtgttcat atctttaaga gttgttataa ggcatggcat
    31561 tttaactttt aagtcatggc aacaatcttc aaattgtctt tttactaata catattcacc
    31621 ttcagttttt atttcttcag catatgttat tgatggaaga tttaaatgtt cagctatttg
    31681 aggtccaact tgtgcagtat ctccatctat cgcctgtctt ccagctatta taatatcaaa
    31741 atctatattt tttaatgctc ctgctaatgc tgatgaagtt gcccaagtat cagcacctgc
    31801 aaatgctcta tctgttaata atatacctct atctgcaccc attgctaaag cttcttttaa
    31861 agccatatct gcttgaggag gtcccattgt tataacagtt acatgagcac ccatttcttc
    31921 ttttaatttt atagcttctt ctaaacctgc tttatcatca gggtttatta tacttggtac
    31981 tccatctcta attaaagtac ctgtattagg atctagttta acttctgttg tatctggaac
    32041 ttgttttata caaacgacta tattcataaa tccttcctcc atatctattt taatatcgac
    32101 ctattgtgta tattttaata tttatctatt ttaataattt tcctgaaata accattcttt
    32161 gaacttcact agttccttca tatatttcag ttatcttagc atctctcatc attctttcta
    32221 ctggatagtc acgagtgtat ccatatcctc catgaagttg tacagctttt gtagtaactt
    32281 ccatagctgt ttcagctgca aataattttg ccattgctgc ttctactcca taaggttttc
    32341 ctaagtcttt atttatagct gcttgatata caaggtgtct agccgcttgt accttaactt
    32401 ccatatcagc taattggaat tgtgtatttt ggaattttga taatggtcta ccaaattgta
    32461 ctctttcttt tacatattta acagtttcat caagagcacc ttgtgctaaa cctaaagctt
    32521 gtgcagctat accaattcta ccaccatcaa gagtagacat tgctatctta aatccttgac
    32581 cttcttttcc aagtaaattt tctttaggta ttctgcaatc ctcaaatatt aattcactcg
    32641 tagctgaacc tcttataccc attttctttt ctttaactcc aaagctaaac ccaggagttc
    32701 ctttttcaac tataaatgct gatattcctt tgttcccctt agatttatca gtcattgcca
    32761 ttactacata tatgtcacca gctattgcgt ttgttataaa tatttttgag ccattaagta
    32821 tgtattcatc cccgtctaaa acagcagttg tttgttggcc agacgcatct gtaccagcat
    32881 taggctcagt aagaccaaat gctcctaatt tttctccact tgctagtggt cttaagaatt
    32941 tttgtttttg ttcttcatta ccatattgat atataggcca tgagccaaga gatgtatgag
    33001 ctgataatat aactcctgta gtaccacaaa ctctagacaa ttcttcaact gccattatat
    33061 atcctacagt gtctccacct tctccaccat attcttttgg atatggtata cccatcattc
    33121 ctgcttttgc cattttttcc actgtttcat aaggaaatct ttcttcttca tcaagttctg
    33181 ttgctaaagg tttaacttca ttttcagcga agcttacata tagctcttta agcatctgat
    33241 attttttaga atttaaatcc ataatagcct cctcagtatt ttataaatat tttattttca
    33301 tttattaatt aaaatctcgg caacatctat aataaggtat ttgaatatac gtcgagaaat
    33361 ttataacctt aatcaaacta cgctctatat ttatatgtga ttatactaac caattatgac
    33421 attaatttaa tcataaattg ttaatttaaa atcaactata cttaaaaaaa atctcgcttt
    33481 ttggaatttt gtgaaatttc aagttagaat aagatttctt tcacactggt tagagataaa
    33541 ataatttgtt tacgatattt atattagggt atattaaaca ttaaatattg tctgtacatc
    33601 tgttttttag ccatgtacag aagattttta tcgtataaaa attattttaa tagagctaaa
    33661 taatcatatt tttatctgac tattttttat ttttaaatat cttacatcta taatagtatc
    33721 actcaaagga taatttttca ataatttcta attatttttt taacaatatt tattacattt
    33781 tttccacttt tttgtcattt ttttattgtt tcgaaaaaaa atcagaattt tttaacttta
    33841 ttttggatta ttagtttaaa ttagattttc ctcttagatt tataaatatt tacacaaaaa
    33901 aactacctct gtatagattg tatacagagg tagtttttta ctaattccta ctatagttta
    33961 ctatattttt ttaccaattc ttacaaacat ctacccattt ttcacactta gaaaacttct
    34021 ccatttcttc acatgtaagt tctattgcag agttactact gccacatgca ggaaatactg
    34081 tatcaaatct tttcattgaa tcatctaaat agacttttat agaatttttt aaaccaaatg
    34141 gacagactcc accaatagca tgtccagtaa actccaagac ttcttctggt gtcaacattt
    34201 tagccttaca attaaattca gccttatact tcttattatc aatcttagca tcaccagctg
    34261 tcacaattaa aattgcatca tctcctattt taaatgagag tgtcttagct attcttgctg
    34321 gaataacccc agctgcttca gctgcaagtt ccacagtagc actagattgt tcaaattcta
    34381 atatactgtc ttccttgcca aattgtttaa aatactctct tacattactt attgacatta
    34441 aatacccctc ctagtctgaa cttttataaa tttcatatta ttcttaatta tatctataat
    34501 tgatttattt ttcaaattat tccataatat tagcattcca tgtatcatta ttttttccaa
    34561 ctactagata aagtcctttt gccattggaa tattgacaat atattctcct ttatactcta
    34621 cactcatacc agattcatta aagtacctat aatcaaaatt tagtcctttt ggaattacaa
    34681 aatcaacata accttgatta tttggtttta ttccatattt cagagttctt tcagttatag
    34741 aatttttttc attaaataaa tctacattta catatgcaga acacttattc attccctttt
    34801 tattttccac acaattttca aacttcattt tagcactgtt taatttataa ctatatttac
    34861 tattattgct tattttaaat cttacaatag taccaacatc tctacttctc ctgctatcaa
    34921 aatcaaaaac aatttcctct aaatgattta tttcataatc tttgacactt accatatctc
    34981 tatatactaa tttatttata gctatgaata gtgtaaaaat ccccaaaata aaaaatgaaa
    35041 cttttataaa cttcttcatt tttagtctcc ttatattttg tgctattgtg ctataatatt
    35101 aatcaaaatt ttaataatta aacttattct tttttgaaat cttaatagaa aaatacattg
    35161 atattataaa aattattatt gttaaaaatg tcatactata taaatttata tacttagtat
    35221 tagctatatc agttacataa tttgaaagat atactcctcc aactttacta taattaaaat
    35281 gctttacttg atatacaatc atactaatca tcttcaaaag acttccatat acaaagtaaa
    35341 ttaaaatact tactataaaa ccacaaactt tcatttttcc atatccaaac ttaaaaatta
    35401 aaggcagtaa aatcgtcaaa attgctaaaa atatatttac agaaaaaaat acatcattta
    35461 aaaccatatt tctaaagaac actagtgatg tcaatttact tagtaaggac acaattattg
    35521 ttacagaaat tattaatcct acacctaata aatattttga gtaaactata tcttctatac
    35581 taactggcat actccttata aaaatccttg aatcattttc ataatcacac tcaaatgtat
    35641 ttataaatat caaataacta acaacaatac ttactgtata atatgaaatt gaattaagga
    35701 aaaaataaaa cacaaaaaat agcaatatgt actttaaatt acggatacca tctgaactaa
    35761 tccctattat taaatctttt cttattaaat tgaacatcat ctcacctcat ttacataata
    35821 atacattagt tcttctatag taggattttt gaccttatgc ttatcaattt ctatcaatag
    35881 gctttttact ttcaagctat ctccaacttt aacaagtgcc tcattataat atttatactc
    35941 tttcacacca actatgcttt ctttaattgc ttctaactct gctttatttg ccctaattat
    36001 cttatattct tgttctatta attctttctt aacagtaaaa atctgatttc ctctatttat
    36061 aaaaacaaga taatcagcaa ttttaattaa attatcagta ttatgagtag aaattattac
    36121 actactactt ccatttttca caaaatcttg taagtactgc attatttcag ttcttacaat
    36181 tgggtctata ccagcagttg gttcatccaa aatcaataac ttagcattat gactaagtgc
    36241 gcttgataac ataagcttct cagattgacc tttagataaa ttttcaatat gcatattttc
    36301 ttctatatca aacttattaa ggtattcatc aaacctttct ctatcaaaat tcttataaaa
    36361 taaagaaact atttttctaa attccttaac ctttaaatgc ggataaaatt gtaaagaatc
    36421 atatacaaaa ccaatattgt ttttaaagct tattggagaa ttttcaatag tttcattaca
    36481 aagttcaatt tcaccttcat ctgcatcaat aatccccatt ataattttta ttaaagtcgt
    36541 ttttcccgaa ccatttggtc ctataatccc aacaatatcc ccttccttta actctaagtt
    36601 tatattattt aacttgaatt ttcctaattt ttttgtgata ttccttatgc taaacacatc
    36661 ttcacctctt atgcattttt ttatacccac ctatacatat tttaacacat tcactacaaa
    36721 ggtcatttat aaatttataa attaagaatt ataaaactat gttataatac aaagtatgaa
    36781 gaggtgaata tatgaaaaga attataacta taaatacaaa ccaattaaat aagtcccttg
    36841 agactaaaga actcttaact agaaaactta tcaatgctgg ctttgaagtg tattcagata
    36901 tttatccaga tacagagctt attatatcaa ttggtggtga tggctctttt ttaagaacag
    36961 ttagagattt cgattttcct gaaattccca taatgggaat aaatacaggt catctggggt
    37021 tttttcctga tattttacca gataagattg atagctttat tgaagcttat acaaaaaaag
    37081 attatattat tcaagaaatg tcacttctta atgcggaagt atatacaact actagtggtt
    37141 ctaatatgct tgctgtaaat gaggttgtta taagaggaga caagtctagg actatacacc
    37201 taaatttaag ccttgataat aaacatatac aaaattttag tggagatggt atgattattt
    37261 ctacttccac aggttcaaca gcctataact attctgctgg aggtagtatt gttgatataa
    37321 atcttgaact tatgcagatt acacctttac atccgataaa taccaatgca tataggtgct
    37381 ttacatcaag tataatatgt tcaaatgaat ctgttataaa gattgcacca gaatatagat
    37441 ttgaagattc tgtattgatt gtcgttgatg gagttgagca cagatttaga caaatcgaaa
    37501 atataaaagt tagtatatct gatgcaaaaa taaaattact tagaatgtca aactatgagt
    37561 tttggcatag agtatctgaa aaatttttat agactgtact taggaggatt tttaatatga
    37621 aaataggaat aatgagtgat actcatggta gcttacttta ctttgaaaaa gcgttaaatg
    37681 tattatctga ttgtgatgtt ttattacatg gtggagatgt cttatatcat ggacctagaa
    37741 atgatattcc agaaggatat aatcctaaaa aatttattga gacattaaat aaattagaaa
    37801 acattgtgat tgtaaaaggg aactgtgatg cagatgttga ccagatggtt atagaacatc
    37861 ctattcagag tccttatgtc atgagccaat ttggtgaaat tagaattata ttgaatcatg
    37921 gttatataga atcagaagaa gaaattattg acaaagcaaa gaaaatgggt ggtgacatat
    37981 tagttttagg acatactcat gtaaaaaaac tatacatgga tgataattta atagtaataa
    38041 atccaggaag tacttctatc ccaaaagatg gaagccattc tgtcgcaatt atagatatca
    38101 taaaaactga tgatgaggat gagcttgaac ttgatataaa cttaattgac atcaatactg
    38161 gaaatataat aaatatcaat gattagtaaa tttttaataa aaataaaata agctttaatt
    38221 tattaaattc ataataaaaa caagtaaacc attttatttc tatctcaatt ataagtttaa
    38281 gtacaatacc ttaaacttct aattaaatag aatctggttt acttgtttta ttatatgcta
    38341 tattgaaata taacttagtt tacttgtttc tattatatat tatactgaaa tataacttag
    38401 tttacttatt tcattatata ttatactgaa atataactta gtttacttat tttattatat
    38461 atattgaagt gcaacttggt ttacttgcta ttattatata ttaattttga tttacacaat
    38521 attactgtaa ggctaatgct tctgcatatg gtttactatt tttacctcca ccaacttgta
    38581 caaatttatc agcctttact ttttttataa aacttttctg tgcactactt aaacttccac
    38641 ttgcaagtac tacaggtgac tttgtttttg atgctaatac tccagcagca agcccatcaa
    38701 ctaaatcagc ttctctacca cttccatctt ttgcaaaata catattatta accttacttg
    38761 attttccata aaacttttct aaaactttcg cattagtatc ttttctatct gtccctgcta
    38821 gtctttctga gtttactaac tgtccttcaa gttttttact tatactgtac tccccaccta
    38881 ctatgtatgt cttagttgag ttttgtgtaa taaaattctt tgcctcactt cttatgctat
    38941 ccttacctgt atatattata gctgtattat cacttgcagc tgggcttgat attgaagttg
    39001 catctgccag tccattatac ccattaacca ctgccacttt tgagacacct aaatcaccag
    39061 tatcatctag ctgtctagta agaagtaaag atgtttcttc tctatctgct ccaccgattc
    39121 tactgactaa tacttccata tcttcaagag attcttttac acttgatgaa atagctcctt
    39181 cacttccaat taaaataact tctgctggat taagcctttt tatttcctct attacattat
    39241 atggtatact atttttattt gtaagcagta ttggtgcatc caatttactt gccaaaggag
    39301 ttgaagccaa tgcatctggc aatgaatttg agttaactag aaacaccctt tctgaaccat
    39361 tattccaacc actttgagat atttttatcg ctgtatcaaa tctatcttca ccaataagtt
    39421 tttcctcctt tggagatttt ctaccttgaa gataattgtt catatctacc tgtgcatttt
    39481 gatttttact aatagtgtct gcatatacac aacttggcaa cataatccca agtgaaagtg
    39541 caaatgctat tattttttta gatttaattt tcaatacaat tccccctttt aaatgttaat
    39601 acctattgat attcatactt tttattgata ccctaaacta aaaataatta tcttttataa
    39661 aaataattac aaaaatagct ataccatttt aaatacaaca caagcttata atcagcttga
    39721 cttataaaga atgatatagc tattataact ttaaatgctt aatttttaaa taccttttaa
    39781 ctaaactctt tcagcatatt tttcagctat tcttacaatt agtttagtag ctttctccat
    39841 agaactaact ggtatacact catttttccc atggaagttt aatccaccag taaatatatt
    39901 tggacaaggt aatccattaa aagataatct tgctccatca gtaccacctc ttacaggaac
    39961 taaacgtggt tttataccta attcctccat agcttcttta gctatatcaa ctataaacat
    40021 aactggctca atcttttctt tcatattgta ataagagtct tttaaatcca attccactct
    40081 attatcgtat ttcttattta ttttttctat attctctatc ataatagatt ttctattttc
    40141 aaatttttct ttacaatggt ctctaataat ataagccata cttacattct cgacatttcc
    40201 acctatagaa tttaaatgcc aaaatccttc atatccttct gttgtttctg gtctttcatc
    40261 tgctgggaac atttctgata tttcagcagc tatatgtata gcatttacca ttttattttt
    40321 agctgaacct ggatgaacat ttctaccatg tatagtaata gttgcatttg cagcattaaa
    40381 attttcacat tcaagttctc caactattcc tccatctaaa gtatatgcat attttgctcc
    40441 aaatttttca acatcaaata aatcagcacc tctaccaatt tcttcatctg gtgtaaatcc
    40501 tatttttata tctccatgct taatttctgg atgatttatt agatactcta ttgcagtcac
    40561 tatctcagct ataccagctt tatcatcaga gccaagtaga gttgttccat cagtgactat
    40621 taaatcgtca cctttaaatt cttccaactc tggagaatcc tttacatacg taataacatt
    40681 taacgcttca tttaaaacta tgtcttttcc gtcataattt tctattattc ttggtttaat
    40741 atcttttcca ctaacatctt caattgtatc taaatgtgat atgaatccta tagtatccac
    40801 accatcagta tttcctttta aagtagccat tatataacta ttttcatcta aactcacatc
    40861 atcaacaccc attgttttta gctcttcaac tatatactta gctaaaactc tttgaccttc
    40921 tgaagatgga cagttctcac actgagaatt agatgttgta tcaaatgata catattttaa
    40981 aaatctttcc actacttttt gtttcacgta tataccctct ttcttaataa gattaatatt
    41041 tttttattta cctgtgaata aaattttatt tttaaaaaaa tctatcaatt attataatta
    41101 aaaaatatcc accttcttaa gtatatcatg catacatacc aaacccaaac aaaaaattac
    41161 tccaattatt ccttttactt ggattccaat aaaaatcgct gctagggtag ctaatggatt
    41221 tagtccaaca tttactgata ctagtttagg ttcaagtatt tctctaatca aagacaaaac
    41281 aaaaaatatc acagtcatac ttatagctat aaaataattt tttataagaa aataataaac
    41341 aattactgga agatatattg ttattatacc taggaatgga attaaatcca ttaaagctcc
    41401 tacaaaccct actattagac cgtatttcaa tccaaataaa gcaaaacttc cccagattgc
    41461 aaaaaatgtt atagccataa gtattgtata ggcccttata tatccaacaa gtgaaagccc
    41521 tgcctctttc tttacgtttt ttaccttctt tctaacatca acagtaaaca tattataaaa
    41581 actattttca atcttatcaa ggtcttttgc tataaagtac gtagcaataa acaaggtaat
    41641 tataaatatt ataatatatg gaatagaagt agccaatcca agtatactag taagaagatt
    41701 ttttgtaatt tgcattactt caccactgaa tttgtttact aattgttcta catcaaaatt
    41761 ggctatttct tgaaatgatt tcataaagtc atttatttta tttaaaaaat acataatagt
    41821 attacttata tcctcaggat tagcagatat attatttaaa aatcccatta attctttcat
    41881 tgaatttatt actattattg tagtaactgt actgactata gccactgcaa gaaaacttag
    41941 aacaagagta gagatacctt tatttatacg aaacttattt tctaactttt gtgatatagg
    42001 attaatcata agtgctatta tagcacctag aaaaaatgga gctatgtatg gaaacgcttt
    42061 gtaaataaaa ataaataaca atgtatatat acaaaaaaag atagtgttgt tttttaattt
    42121 gtataaaaaa ttattttcta gtatgctcat cttaaaaatc tcctttcttc taatttatat
    42181 ttttattttg tccatttcat actagtttta tttgaataca aaataaaaag aagtgtataa
    42241 atataaaaga tatcttttat atttatacac ttctaataaa tttaagaaca ttattacatt
    42301 atgtatatat tcttattttt ttaattctat ttttttctat actttctata caaaatctta
    42361 tattatcaac ttcaattact tcattttctt ctggcaatct gcttaggtgt ccaattataa
    42421 aacctcctat ggaatcaaac tcctcagatt ccaagtttac accaataagt tcattaacat
    42481 caccaatttt agtacttcca tctacaatat actcatcttc ttttataact tgtatttcat
    42541 cctcttcttc atcatattca tcctctatat ctccaacaat gacttctact aaatcttcta
    42601 tagttaaaag tccagatgtt ccaccatact catctacaac tatagccatc tgactttttt
    42661 caagtttcat ctcttcaaga agttgtgtta tctttttgaa ctcatacgtg aaaaaaggtt
    42721 ctctcatata atttttaata tcaaatgact ctatttcttc atcagataaa aatataatat
    42781 cttttatatt aagtattcct acaatatcat ctatagtttc ttcatacaca ggcattctac
    42841 ttaatttttc agttttaaat acctgtatta tctcatcata actatcttcc atatctatag
    42901 ctaccatatc tattctttgt accattgcat ttttagcttg catatctcca aattcaaaaa
    42961 cattatttat gatttctctc tcttccattt caaggacgcc ttcttcatga cttacattta
    43021 ccatagtttt tagctcttct tcagttatga atgatttagc tcctttattt gttataccaa
    43081 ataatttaaa tataatattt gtaattatat taaatatcca tactattggt cttaatataa
    43141 atataattgc ttttataggt ttagatacta atagagaaac cttttctgaa ttatttgctg
    43201 ctattgtttt tggagtaatc tcaccaaata ttaatactaa tactgtcata acagctgtag
    43261 caagtgccac tcctttttct cccattaatc ctataaacaa agatgttgat atggatgttg
    43321 ctgctatatt tacgacgtta tttccaacca atatagaact taaaagctta tttggatttt
    43381 ctattaatga acttactaac ttagctcctt taactccttc atcttgcata tatctgattc
    43441 tgattttact caacgacatt aaggccgttt cagatgctga gaaaaatgca gaccctataa
    43501 gtaatactat taaaaaaata atctgaatca aattattggg cgattccaac acttaccaca
    43561 ctcctactta catgttttta tattaatatt aattataaat aactttaata ttagttttta
    43621 ttatatcaca attacatatt atttggaaat attagataat atattcaatt aacatatatt
    43681 ttctaactat attcaatttt tacttttgaa cctattccag cctcactaga tgaaactaat
    43741 agcttaacag gaactctatt tttaagttcc tcgacatgac ttattatacc cacacttaga
    43801 ttattactat gcaatctttc caatgattct ataacaattt ctaaaagttc actatctaat
    43861 gaaccaaaac cttcatctaa aaagaaaaat tctaatggtg cacttccctt taattgtatt
    43921 tgagatgaaa gagcaagtgc taatgataga gatgtcaaga atgtctctcc accagataga
    43981 gtatcaatgc ttcttctttc tcctccatta aagttatctc tcattacaaa gtttagtgtt
    44041 gagtcaattt caagtgcata tcttcctttt gttattccat ccaatcgttt tgatgcttcc
    44101 aacgctatat attttaattg gtttgtagaa acatattcta caaatttatt tccttgtaca
    44161 catttatcta attcttccaa taagtccaca ttgtgttcaa ctaccttaag ttgtttatta
    44221 aaatcattta tcttatctaa agagtctttt aatgttatta actgattttg attagctcca
    44281 atatctttgg atatatttcc tatttctact ttcaaattat aaatttcatc ttttaactgt
    44341 tcaaagtact ccttctttat gcttcttcca tttaattttt cttttgatga ttttatttta
    44401 aatgataata acttttgttc ttcttcatgc tctagtattt cttcaatcaa tttttttact
    44461 gcatcagtat ctaataaagc tctttttact gcatatatac tttcaaattt attttctgct
    44521 aatagtttat ttagtgttga tttttggtct ttatattgtt ccttagcagt tttaagtcta
    44581 ccatctatat tgtgcttttc agcaatgttt ttttcatatt ctaatctttg ctcttccaat
    44641 tttttcttac ttgattcttc ttgttctaat atcttacata tattttcttc aacattatgt
    44701 aaaagatttt tagctaaatc acctttagtt atgctaataa cttctttata cttattatcc
    44761 ctactcaatt tcttttctac atataattct ctagccttta ttaattctat ctctatttca
    44821 tgtagtttac tctcattttc tcttatttta atatctagtg agtctctatt ttttaataaa
    44881 tttgaatgcc cagtacttag taattctaat tctttttcat tctctcttat ttcttttact
    44941 tttgacaata gattagagac tttagtaatc gtctttaatc ctaggtattc ctgtttaagt
    45001 tttttacatt tattttctaa accttctatt tctatgttta aatctcttgt taatttctta
    45061 taattattca gactctctct tatttttaat tcttcttttt caattttatt tttttcttct
    45121 ttcgctaatg ttactttatt ttcagtactc tctttttctt tctcccactc ctgaatatta
    45181 gattttaaca atgcaagttt tctttgttct tcatcaagtt tttttaataa ttgactagat
    45241 ggaatccccc ctagttttcc ttttacatct tcaagttctt tagtcttcat tttttctata
    45301 gataaatacc cacttacttt agcatttaat tcctctatat tgtttcttat acttattttt
    45361 tctttttcta atttctccag tttttctttg acaaaagata tattttcatc atatttagtt
    45421 atattatgat tttcatgatg ttttgaccca caaactggac aaggcatatt ttcttttaat
    45481 tcacgtctaa gttcagaggc taaatttaaa tactttagtt cttctaattc tttttctagg
    45541 tcatctctgt tctttctatt attttcaagt ttttcattta gtaaatttac ttccctttca
    45601 gtattaaact tactttcaag tattatcttt aattcatctt gtatactgct tttcttaatt
    45661 tcattttctt tagtattatt tgctttattt cttaattcag taacatattc agattttaaa
    45721 agtaaatcag cactcttacc aggacatttt ttaagaagaa catccaagtg aagtgataaa
    45781 ttttctaaat tacgatttac atcattctta tctttatcaa tatatctaac ttttaaattt
    45841 atattttctg tctctttgag tatttcttct agtttattta gcttttgatt tttttcttcc
    45901 aatacgttgt tgtaatcttt ctcgtattca tatgctagaa atattttttg ttttaattct
    45961 gcacttatat ttactttatc aatctttcct tcaacttctt ttatagattt agtaactgca
    46021 tcttttctgg actctgacac ctgctttact ttttctaatt caactttagt cttatttaaa
    46081 ttaattccac tttcttttag ctcttttaac tctctatcta ataaaactaa ctcttcttct
    46141 aattttattg cttgttgcaa tcttattttt tcttcactca atttagggat ttcttcattc
    46201 ttaattttgt ttattttttt atatctattt tttgtaactt caagctcctg atttaatatt
    46261 gctaatttct tttctaaatt acttactgcg aagctatctt catctatctt tttttctaga
    46321 ttttgaactg aacttatgta tgggtctacc tttttagcgt tactatcatt ttctacaaga
    46381 gcaatctttt cttttatctc actatttttt aaatcaagct cattttttct aagttcattt
    46441 ttttctaact ttaattgttc ttcatatact gttttacttt cttcataaga tttttgagct
    46501 aggtctaaag cattattctt atctttttcc aaatttttta actctattaa ttcttggttt
    46561 atattgttat atacttcctc tgtaattcca tcatattgac ttaattttga tttcaaatct
    46621 tttaaatttt gtaactgtat attttttctc tttttcactt tttcgccaag acttcttcca
    46681 tatttctcaa gattaaaaat tctctctaac atgtctcttc tatctgcacg tgataatctt
    46741 agaaactcat taaatttgtc ttgtggtaaa actactgacc tagtaaaatc atttgccgtt
    46801 aatcctacga cctgcgctac tttttcattt acttccacaa ctttatcagc taaaacattt
    46861 tgggtattgt cattcaatac ttctattaat ctagcattag aggtctttgt tcctgttttg
    46921 cttctagcaa tggttctatc tacaatgtat cttcttctag tgtttttgct tcctatctca
    46981 aactcgtatg atataattgc cttatcgcac acactgttta tatattcttt tgtatttctg
    47041 gatatatttc catacatagc aatagttatt gcatctaaga ttgtagattt tccacttcca
    47101 gtagttccaa atatcccaaa caaacctctt tcaattaatt tttcaaagtc tataacttgt
    47161 ttatcaatat agctattaag ccctgtaagt tctaatctaa ttggtctcat ctgctgattc
    47221 accttcttca cttattatat ctagaaataa atccataagt tctccttttg gctctacacc
    47281 tttactaaaa gaatagaact ctctaaacaa ctctgccata gacttttctt ttatatctac
    47341 actttcttgt tcataacaag atgttattat tggttttatt tctattatgt cttttaatag
    47401 ttccttcatt tttttaattt cattctgtga tattatctca tcagtattta tttcaaaata
    47461 tgaccaaatc tctctatctt gattttgctc acatatatct agtgcttctt ctattccatt
    47521 acatttaaat acttctatag gtttgtaatt attaaaatat acatcttcaa ttattggttt
    47581 ttcaccagct tttatatcca ctatgtatgc ccctttggta tacgctcttt catccttgct
    47641 atattgaagt ggagaaccag aatagtatgc atttaaacgt tcggaagctt tttgttgttt
    47701 atgtaaatga cctagtgcag tatattgagc cttttctggc aaatcttttc tttcaactaa
    47761 aaaacttcca cctaattgta taggtctttc tgattcagta ctctctccac ctattacaaa
    47821 tatatgtgat acggctatat ttatagtatc acttctaaaa ttttcttcta aacttctaaa
    47881 tatatctcct accttttcag aataattttt ttgtttctca aactcatctt cacttgaaaa
    47941 aacttcattt aatctttttt cacttggata aggtaatgta gcaaccacaa tattttctcc
    48001 atttatattc agttttgtac atccctgagt tgcatatgtt atttcaaacc ctttatactt
    48061 agcttctatc gtagcactta atggataacc atatataagt attccctgtt catgtgctag
    48121 aggtgttatt gcagagagtc tctctggatt atcatgattt cctgaaatta ttaatacaca
    48181 cctctgacca ttattggcaa gtcttgatac agtctgataa aatagttttt ctgcttgtgc
    48241 tggtggatta gaagtatcat atacatctcc agcaattata accatatcta tttcatttga
    48301 ttctactatt tttataaatt cctcacaaaa ctttgcttgt tcctcaattc tagaatggcc
    48361 ttctagggac ttccctagat gccaatctga tgtatgaata aatctcattt tttccccttc
    48421 ctctatcttt taggtttaat ttatatataa aaactaataa caaacagctt catcaactcc
    48481 aaataaatat atacatttct cttttactga cttaccagtt aaagtttcaa taatatctgc
    48541 atataaatcc aactgttttt tatatttctc tattatttga tttatatttt cctcatttac
    48601 aaaatctgtt ttataatcca ccaaaactat ttgattatct tcttcaaaat aagcatcaac
    48661 tatacctctt aacatgacac tttcattatc ataaagtttt ttatcatcat tgtttataag
    48721 cttttcataa atatatatgt ctttcatatt tacttgtgca taaattgatt tttctctatt
    48781 tataatttca gcattcaaca ttctttttcc tatgttagag gcaaaaaatt tatatatttt
    48841 atatggattt actatactag cttgtttttc tgttattata ccttttgaaa caaaacctct
    48901 tatttgtgat tttatatcat ttacactact tacatttttt aagtctaaaa cctccataac
    48961 taaatggact attgtacctc tttctgttcc acttattttt tctctttctt cttcattttg
    49021 aataaataga ggctttttta atattacttt ttgttcaaat atagtattta ttaactcttc
    49081 ttcataatta ttttgtatct tttttatttc tgtaacagat atagtagcag gttttcttgt
    49141 actaaattca tatggatata tatagtttaa tttttcttct atttcactgt aataatcact
    49201 gtcaggatta tctacatcta ttttttcaag aatttcttca atactttctt tttcttcatc
    49261 actcttcttt tccactaaaa tatcattctt attccacaac tttccatacc attttgaatt
    49321 atgttcaaca ttaaaaactg cgtctaatcc tacttcttct agtagattag ataaatccct
    49381 atgtcttaat acacaaggca ttatccaatc taaaaaattt ttacctttta atatctcgta
    49441 ttgtgatatt gtatctaaac tttcaattcc atttgaccat ctctttatac tatcttgtat
    49501 atttctagta gaaccagtta taatcaattt ttctttagct cttgtaaatg caacatataa
    49561 aactctcatc tcttctgata aattttctat atttattttg ctttttaatg cttctttggc
    49621 tatacttgga aaagaaatcc ttctctcata gtctacaaat tgaggtccat accctaaatt
    49681 atgatgatat aaaatactct ttttaaaatc ttgagtattg aaatttttac ccattgcaga
    49741 gcatatgact actggaaatt ctaaaccttt acttttatga atactcatta ttctaacaac
    49801 atttgcattc tcaccaagtg tttttgcact tcccatatca gaactagatt tttttaattt
    49861 ctctataaaa tttacaaagt tgaatattcc tttaagactg gtttcttcaa attgttttgc
    49921 tctctcaaac aatactttta aattagcttg tctttgactt ccacctggca atgctccaac
    49981 ataggcatag tatccagtcc ttgtgtacaa gtaccatata aattcatctg tactcatgta
    50041 cattgacttt tctttaaatt cctttagctt aattaaaaaa tctttacttt tgtttatgca
    50101 ctcttcactt ggtataaact cagactcatt ttcattttga gaatctgtaa atccatcata
    50161 ctctgctgta ctttttaaaa cctcataaaa aattttatct ttactctgaa ctctaatatc
    50221 aatcaaatcc tctggtgtaa atccaaatat aggtgatttt aatacagata taagaggtat
    50281 atcttgcatt ggattatcta ttatttgaag taatgacaat attgttttta tttcaatcgt
    50341 atcaaaatac cccactccaa catcagcata tgttggtata tccatattca tcaattcatc
    50401 tgcaaacact ggagcccatg cagatgtagc tcttaaaaga ataactatat ctctaaattc
    50461 cacaggtcta tatccatcta ttcccttatc atagaccttt tgaatttttc catcttcatt
    50521 gactttcatt aaatctttga taatgtttcc aaccattcta gcctcaagtt gtatattatc
    50581 taatttttct tcttcctcta tttcattttc tttgttatta attctatcgt ctttatcatt
    50641 tataatatca tcatcaagtt tattatcttt ttgtattagg tgtatctccg ttgctccacc
    50701 aataatagat ttctcgtctg tatccacatt aaaatttgca cctaaattta gtctttcttt
    50761 ttctgtatat tcaatttctc ctatattctc attcattata ttttcaaata tataatttac
    50821 agcatctaca acttcttctc tacttctaaa atttttgtaa agcattattt ttctatggga
    50881 acttcccttt ttatcattat agttattata tttttgcaaa aataattctg gctttgcttg
    50941 tctaaatcta tatatactct gttttacatc accaaccata aatctatttg gagtttctgt
    51001 atttgcaaca gctttaagca gtacttcctg cactagatta ctatcctgat attcatctat
    51061 aaatatttca taaaacttgt ttctatatcc aactgctata tcagaaggta caatattacc
    51121 tttttcatct acatctgtaa gtatatttaa tgcaaaatgc tctatatcgt taaaatctat
    51181 tattcctttt tctcttttct tattgctata ttcctcttca aatctcagta caacactaga
    51241 tattggttta actatgttat aaagatattt tatttcttct ctaatagaat cattatcttt
    51301 attgaatgta gcacttacta tactttctaa agatttctta gtcttatctc ttatagtttt
    51361 tgccttttct tttgattctt ttatatacga aggagcatct tttgaaattc tttttactcc
    51421 ttttacataa ttttcaaagg acatacttgc catcttttta tatgcctcat cccaagattt
    51481 attacatgct tgtgaaatgt cagcaatttt tttatattca acggataact tttctgcaaa
    51541 agtttctaat tcttctattg attccacttc ttttaatgcc ctttccatat ttagacaaag
    51601 accattaatc tctattttta cagtatctaa tatagctcta gcccaaatag actgtgaaaa
    51661 atcaaaattt tcatcaatat taaatcgttc tgctgagtca atcaaccatt ttttaggttc
    51721 aggagaagcc attgcaaatg aatatattcc caagataata tcttgtactt ctttatctcc
    51781 tcctctttct gcataacttt caactaaatt taaaaacccc tcatctcttt cttcatataa
    51841 atcttcaaat acttcctcta ttgcttcttg tttcaaaata gcacattcag tttggtctcc
    51901 tattctgaaa ttagggtcta agtttattct atgaaagttt gattttataa catccaaaca
    51961 gaatgagtgt attgttgtaa tactagattt atttaataaa acaagttggt tttgtaaatg
    52021 tttattttct gggttttcat ctaaggcttt tcctatagca tcccctatcc tctctctcat
    52081 ttctgacgca gcagcattag taaatgtaac aactaataac ttatctatat caattggatt
    52141 ttctctactt gttatcattt gaattatacg ctcaactagc acggctgttt ttcctgaacc
    52201 agcagcagca gcaaccaata aattgcattc tctactttct ataacttcta attgctcttt
    52261 tgtccattta ggagaactca ctaattaaca tctcctttca tcatttttat tatctcttca
    52321 ttactcttat tatttaaatt tttatacttg ttatctttta tagttgaatc aaactgacat
    52381 atagcagagt aatcacaaaa gtcacatgat gttccatctt tatgtttata tggagctata
    52441 cgtatttctc cacttaacat ttcttcacat aaatccttta tagcatgcct tacatatttt
    52501 ctaagcagtt tgaaatcctc atagcttatt gctgaagtac ttttacctac atttccatcc
    52561 ttagtaagac ctactggtat tactaaagag tttttccttt ctccatctat taatgattta
    52621 tccatctctt taactatgtg agagtctttt actacaagcc ccttcattct aagttctttt
    52681 aatattgctt cttgtatttc ttcatcatct ttatcttcat taaattttgc tattggatta
    52741 tttattctac aatatagtat tgcagctgga tttatattta tatcttcatc ctttgcactt
    52801 tcaaggattg catctaaata cacaagtaat tgtaattgta gaccataata tatttctgtt
    52861 agacttatgg acttattgcc tgacttataa tctattattc ttatatattt attttctcct
    52921 tcttcaaatt catcaactct atctatttgt ccaattaaac ttacttcttg tccattttct
    52981 aacaccattt ttataggtgg atattttcca ttgttgccaa atttaacttc ataatctatt
    53041 ggttcaaatg aaccttgttt tatttgctga ctaattatag ttatagcagt tgtcaacata
    53101 ttctttaatc tatatgcaag gtatttatac ctctcagaac tatttaatat atatcctggt
    53161 atctttaaaa ttatttcatc aactattata cctatcttta attctatata tttttcatca
    53221 atatctctcc aagttaaatt atcttgtaaa agttcttttg aaaacctatc taaaatatta
    53281 tgaataaaag ttcctaaatc tggtggtgta aatgaatact ctttacgctt ttttgctttt
    53341 agaccatatt gtataaaata agcaaatgga cattcagcat atttttcaag cctagatatg
    53401 ctaagagaat tacttttata caatgacctt atttttttct cttcaatctt atgaacttga
    53461 ttagtataac taagaccttt tataactttt tttgttatag aactatagat ttcatctttt
    53521 aaatagtacc tatatatatc taaccaaata ctattaatct cttctgtatt gtaaccatca
    53581 ctgtcataat tttttattac gtttataagt tcattaaatg taggggattt aaccgttatt
    53641 tttttaagta tatctttatc agtgctttta ttttcttcca ctaaataact tttattttct
    53701 atatttggaa atatcttctt aagtcttgat ataattattg atggtctaag cgttttacct
    53761 tcatggtctg atattggata tgttatggtt aaattcttag atgtcgaagt aagagcttta
    53821 tatacaagaa actgttcttc aaaactccta gtcttactat caatatcaac ttcgattcct
    53881 ttgtttccaa gactttccct atcattatca ctcaatattc cactatcctt tgtaatcagc
    53941 ggaaacacac catcagtagt tccaactagg tataaatatt tcgtgtctgg atttttcatt
    54001 ctatccacac tacttacaag tacctggtct atactaggag gaaccagtcc taattcatat
    54061 tcatcaaaac ctaaggttat taattttata aatttttcta aagaaattat ttcatcaccc
    54121 ataagttcca ccatttggtc taatatgtcc actactatgt cccaaacttg tgagtattga
    54181 tttgcaacat caagttcccc tttatcctta aaatttatta ttaaactttc tatagtttct
    54241 ggcatattta tatctaataa aaattcatat atatatctac agatttcttt tactctattt
    54301 tttcctctta atttttcttg taaaattact ataggctcta atactctatt ttttatttca
    54361 tttattttct cttttaattc cagttcaaat tcactttctt gacctgaaag actttgggtt
    54421 attctataat cccatttttc atcaaaccat tttttacctt taatcccatt agccaacaca
    54481 taattttcta gtaaatttat atcatcatta tcaattccta taagtccact ttttaaatac
    54541 ctaaacatag tttcataccc atatcgtcta ttcttcattt ctaaagctga aataattaaa
    54601 ataactattg gattactttt tgcctctctc ttcttatcta taaagtttgg tatattatat
    54661 tcattaaata tagagtgtac taaaaaatca tatctattta aatctcttgt agcaatggtt
    54721 atatctctat atcttacatt cttatctcta actaaatgta caatttctct ggctatctct
    54781 tccacctctg aatataaatt gttaaactct tttatcttta tattttcagt tggatttgaa
    54841 tatattttat atggataagc attataaaat ctttctaagt gctgtaattc cttaactttt
    54901 tcaaatcttt ttataacacc tgtgtttaaa tttacttgtg gcaatatttt tatgccttct
    54961 tcgttgcaca attgagtcaa ttttgaataa gtaaatttcg ttctagaaaa catatcagat
    55021 ttactgtacc caatatagtt gatatcatct acagtcaaag agatattaac acttttagac
    55081 ttatttaata tactctttat gacattgtac tgatttggtg taaaacctgt aaattcatct
    55141 atataaacac aagctccatc taaataactg cttaattcaa ttttacttgc caatgaagtt
    55201 aacatatctt gagcatctac ataattttca tgcaatttcc cttcaaaaga attatatatt
    55261 ttacttatat cttttaattt taaacttaaa gtttcattat ccaattctcc agatatattt
    55321 tctaacattt cagggcttat attgtattgt ttcatttctg atatcatatc tgtaattgag
    55381 cttacaaaac cagactgcga ttttgatttt gaaaacacat ttaattcttc acttacatct
    55441 tcaatagctc tatataccat cattgctttt ccactagaat ttatatttac atctgtcaat
    55501 cctcctactt gagaaaaaac tatattactt agtgtcttaa agctaagtac cctaactctt
    55561 agatacttat ctttttcttt tcctaagaat agcttactta ctctattttc agcctcaaat
    55621 gtgtactgtt ctggaactaa aagtataatt gatgtcgttt catttttttg agcttctttt
    55681 ttaatctcat ctagtatata ggtggatttt ccacttccac ttctacctaa tacaaaccta
    55741 agtcccataa atttccgcct ccaaaatact ataatctatt tctttgccat tacataactt
    55801 attaaattca caatacttgc aatgcttttt atttctatag cacatacgtt cttctttatt
    55861 atataccttt tctatatcat tgtatagatt gtcttcgtat gtatgttctt cataattagt
    55921 gttttttatc atacttatat aattttgaat tttatttcta tttgcttcat gtttttcttc
    55981 atcatataaa atggtaattg gtaagtcatc aaattcaggt tgataatatc tcatatttat
    56041 atctttaaca tttatgtcca tttttaaaac tttaggtatt acttcttttg ctaaaaacat
    56101 ataaacaata gtttgtattc tattttctac gtgtttatag tcaatctttt tagactcggt
    56161 tttccaatcc catatttcta tattgttatt tccaattaca actaaatcat actttgcctg
    56221 tattatatcc ccatttaatg tcatccttac ttcatattct ggcaaatacg tttttccttt
    56281 ttcaatagga acaacttttt ttatattttc catccacttt aaaaactttt tactattttt
    56341 atcatcctca ttaaaataga ttccaagagg tatattgcta aaatatcttt cacaaatcaa
    56401 atgaaaatct cttcctgttt ttaaagtttc ataatactct ctactaccaa catcatcatt
    56461 tttccaattt attctatcaa tatatttata tttaaatttc aatggacatg atttatatgt
    56521 gttaattgaa ttttgactat aagaaaaata ttttaattta tcgttcactc atatcacttc
    56581 tctcttaact ttatttttta gtagttactt actgtacata ttgttttatc cccttttaga
    56641 tatatttcaa gcaatctttt aataacttat tatatattaa tttgaccttt tttcaataat
    56701 atgttgatta agtctgctta aatatataca tggtttttgc tcttttctct tattccctat
    56761 atcactttca tctctataag cacttcctga aagtactaac atttcctttg ctctagtaat
    56821 ccctacatat aaaagtctaa tcttttcatt tatagaatca atcttcgttt tatgagcata
    56881 atctgttgaa atacttcctt ttaatatagc ttcaacttct gatttaataa ttgccatagg
    56941 gtttttatat ttttctttta gataccattt atcactctga aacttttgat taatattgtc
    57001 agggaaatta tattcaacaa gacctagtaa aaacacacaa tcccattcca tcccctttga
    57061 tttatgatag ttacatagtg ttatactacc gggctctggt tcatatccat ttatctcata
    57121 taccacatct attatatgat taaacaccct gttctttaca tcaaacaata aattatatac
    57181 atcttctaag tccatattta tattttctga tactagatac tttatataaa aagatatata
    57241 atccagtaca gctttatctt ctttttctaa attaagtttt ttcccaataa acaaaactaa
    57301 gaggtctaac cttattattg gatattctaa tatctgtttt ataatatcta ttcctctttt
    57361 aaaacttaaa tatatgtcac tttcttcttt aaattctatt aaatcattat ctaacttact
    57421 aaattcaaaa ttataaataa tttcttcagt agaatagttt tttaattctt ctaaaaaata
    57481 ctttatttct tcttcattat cagtatctat aaaaacttta ttaagtacaa ctattaactt
    57541 ttctacatca tcacaatgta acataaaatc aataatatat gctatgttgt ttataacctt
    57601 ccttttattc aaagaatttg gtcctaattc ttcaaaagtt agattattct ctctaagttc
    57661 tttagcaact tgtgtcactt gttcattaaa tggaaccaat atacctatac ttttatctgg
    57721 atatttattt tttatacctt taacatatct agcagtttgc tctatttcaa ttttccaact
    57781 ctcataccac tttacattta tattataatg ttctggatta ggattttctt tatatcccat
    57841 tccatttggc acagttttta tttgcatatc ttcaagagca tgtctacatt ctttctgtct
    57901 aaattccttt gtaacatact tgacaagagt gtttgctaaa tcaagtatat cttttgaact
    57961 tcgatttgac atatccatcc tataacagaa atctgcacta tttataaatt ctttaaaaaa
    58021 tttagggtct gatgatgaaa atgttcctgt tatactctga tttatatctc caactctaac
    58081 taggttttta ttttcgctag atataatttt tattatatta ccttgaattt catttgagtc
    58141 ctgacactca tcttcaaata tatatttgta tctagtttga aacttctttc taagcccttc
    58201 gtctaagctt aaagtttttt ctgctaaaat taatatatca tcataatcta gcaatccatt
    58261 ttgctttaat tttctatcat acaatttata tattggtaaa ataattttaa gcattccttt
    58321 gtgtcctctt gacaatattt cttcaagttt atctggagaa attcccttat acttaagttc
    58381 acttattgca tttcctgcaa attcaaaaaa acctctttcc caggcttcaa gagttatttc
    58441 tttccagcgt tcatcttttt gctcctttaa aaaccatcta aaagctcttt ctccaccttc
    58501 aaatctaaat ctacttatac attcatttaa tataatactt ttttgcaaat catcagctat
    58561 attaaaatcc tcacttaaca ttacaacttc tggcttttcc tttattattt ttacagccaa
    58621 actgtgtata gtcattactt catacgaatt agtatcatct attttattct cttcaagaat
    58681 ctttcttatt ctacctttaa aattatttac agcactattc atatatgtaa gaattaatat
    58741 tttaccaccc ttatgtttct gttcaagtaa tagtttagca actaaatttg ttattataaa
    58801 tgtttttcca gcacctggta ctgctggtac tgccatagta ccattatcgt aatttattat
    58861 tggtatctgg tcttctctat aatttatatt aatcataatt tttatctcct ttatgtgaga
    58921 tttttaaaag tatactatat aatatactat cttggatata accattaatc gcatattcac
    58981 ttttaaatgc atagattttc tttgcattta ataacaaatt atatactgta ttgtatagat
    59041 agtatttttt ataatattct tctatttcat ttgtataaat ctttttttct tcaaaagatt
    59101 ttctaagtac aattaaatta cttatatctt tttctatttt catattccaa gcattacttc
    59161 ctatatctac ccatatttgt atacttctat ctatatttga tgatatatat gaataaggag
    59221 tagttattac tattttgtct ttggatttca actcttctat atcgacaaca gagtaataat
    59281 cgtttattgt actttttaaa gcttctacaa atatcttttc ttttttcatc ccaagtagac
    59341 ttaaactttc actaaaagac tcactttcag ctattatctt tttacataac ccaacattct
    59401 ttttaccttc ttttaaattt aacattttat ctatgtaaaa ttgagttaaa aattcatgta
    59461 tttttatatc actcttcttt ttttcttcaa tatactgtaa aatatcacga tactgactat
    59521 gttcatctat attatcaata cttaaatgtc ttgtttcatt aaaaatctta aatgctttta
    59581 tcctatttat ttctaaaaga gtttctaaaa agcttaagta ttcatcatct tttataaaat
    59641 ctaaatatcc atagaatata catgtagcca ctaccaatgc attcccatat ggataatcca
    59701 cagctttttt atccttctta gtactaaaaa catctatatt tttttctatc aaagaatctc
    59761 taatttgatg ctccaatata ctattgttta taggagatat aattgctata tctcttggag
    59821 atacatcttc ttctataaga gatataactt tttcacttat taaatctaac atttcattat
    59881 ataattgact tgattgattt aattctatat ttgcaggtaa taagtataaa tcttctactc
    59941 taatctcctt ctttttaata ttttcttctt taggcttaat ttttgatatt attttctcat
    60001 ttatatagtc tatatccaca ttattaaata cagaataatc cctactataa tcaaaatata
    60061 tataactttg taatgtatta tccaatgctt ctgttataaa atcaacttct gcattacttg
    60121 agctttcaag gctatccaca attatatatc tcacttctgt aaataagttt tttaaataaa
    60181 aatcatcatt aagtaggtat tgattgtata aatatatgct tagtgcatta tctaaaattg
    60241 aatttgatag taaggaattt atataatatg atataatttc atccatttga gtgtatgaaa
    60301 atctatatat actatcttta tttttctttg atgaatatat tttttctcca ataaaatcaa
    60361 aatcaattaa attaaatgca gacttattta tgttagtcat tatactattt gctatggatt
    60421 tattggttcc tgtaatatct tcaaaatatc cttcttgtat ccttttttcc ttaacttttt
    60481 gcactattaa atattcactt aaactatttg atataaatgt tggagataca acctttgtac
    60541 ttatttcatc acatttttct attattagtg gccagtattt aactaattct ttcttcacaa
    60601 aacttatata tgttgttatt ttcagttctt cactataatt taatcttaga gccctttcat
    60661 atcgtatctt tgtgttattg tttggtacta acaaaagaat acaccttgaa ttattaccag
    60721 ttttatctaa taattctcta tatttatcta aagcttcatt tccatattct tttaaactac
    60781 ttttttcaaa taaatattcc atataatcac cttcctcaat taaattaagt gtatattatc
    60841 taattatatc atattgtgta ttttacaaaa cacaatcaaa ttattgcatt taactacaaa
    60901 aaaagactgt atatatactt gcattcacat gtgcaaatgt acatacagtc ttactattga
    60961 tacacttata tttcattttt tgactttagt ataaatttac attaagtttt taaatatttt
    61021 tatttccatt tattttaatg aatcttctat tcctttacct atttgtgtac ccatttcttt
    61081 tacttttgca gatgagttta ttaaactcat atcattttca ttatccaaaa atgcacattc
    61141 tacaagtaca gctggcatat cattacttct taaaacgtat aaataatctt ttgtagaatc
    61201 actaggaagt actcttgttt ttatacctct atttgtaagt ttaaatttct ctaaaattct
    61261 atttaatata ttggtagcta cagttttagt agtccctcca tttttatctt taagcttata
    61321 aaatacttct acaccatgac cttgtttatt agttgagcca ttataatgta tacttgtaaa
    61381 taaatctggt ttcaatgaat tagatagagc agttctattt ccaagagata aagtcttatc
    61441 tgtgtctctt gtcattatta cattgaatcc ctttgaacgt aaatattcag ttgttgcaag
    61501 tgaagtattt aaattgtaat ctttttcaat cattcctcca gatgaatttc caggtgctga
    61561 accaccatgc cctgggtcaa tcataactgt cttgccccca ctatttcctg gttctgttgg
    61621 agtcgtattg tgtttagata atgatgatgc tatagagctc attaccttag aagtcaatcc
    61681 tcctcctatt ttgtatacct tattagctga tttagcttct aaattatctt tatggtatgc
    61741 agatacatac gtctttggtg ttataagtat tggagatttt aagttcgcag ccaatggacc
    61801 tgctgctaaa gcatcaacaa gtacatctga tttagctact aaaacagcct ctaactcatt
    61861 atctgtatag aattttttta ttacgtttgc atttgtttcg tgtctatctg ctccgtatac
    61921 tctattatta gtaacactat ctttagttat tccatttacc ttatttataa catttgttga
    61981 tatcatttga ggaccaccta taaaataagc attttgtaaa tcctcactct ttaaccattt
    62041 atatgtattg tctgtaatac tatctttatc agttaatata attggttgct tgtcttgacc
    62101 tgcttttgct gctatagtaa gtgcatccac ttctccgcca ttagcatttg ttatgtatat
    62161 tttttccaca tcatgatttt tatctatttc ctttgctatc aataaagatg tctcatatct
    62221 attagaacca tttaaacgtg tttgactagc atttacagtt gatttaattt gattagcagt
    62281 agttttagaa atagcattct catctccaat tataattata tctttagggt ttaggcgctt
    62341 taattctgat tttacactat taggtacatt gtttttttca accaaaagta ttggtgcatt
    62401 atatgtggtt gccagtggag ttgatataat gccatctata cttacatccc catttattat
    62461 aactacctta tctgaaccat ttttccagcc ctctttactt attttaactg ctgtttcata
    62521 tctatcactt cctgtaagtt ctgtaatagg tgaagaagtt tttctcaaat atttatttac
    62581 atcttttcta gaagttatat cttcaattat acattcctgt gttacattat agtcttttaa
    62641 atttaatccc agcttttcta ggtcttgtct gtttaaagtt ttaggattat ttttgctgtt
    62701 tattgttatc ccttgtagtt cccaaacatt tagatattct gggtcttggt tataattgat
    62761 agttccttca tatgccccag ttgattgatt gtaatttaga gttgtattca atggcatgtc
    62821 atagcattta tactttaatg taatgctatc tgcatctggt ttttctttaa atttgacact
    62881 aacttttgat ttgctaccta aaacgatact ttttttctct aagtttatat ttaaatcagc
    62941 gttattttcc atagcattag attcagtagc aaatgccatt gtaggtgcga cgattgccat
    63001 tgaaagaatc cctgttgtta ataattttgt atttcttctc atattaatct ccttacttaa
    63061 cataaatttc tactttattt ttaatctttt aacttttgaa aatatgtcaa agtgtatcta
    63121 acccaaaata taaataatat ataagtaata tataaaaata aacttataaa actcaaaaaa
    63181 taaaaaaatt aaatatattt aatacattta attatctata ctatatcatt tattgacata
    63241 aaaatccaca aaaaacacac aaacacacaa taaaatatag taattaagct tatattgtgt
    63301 attacaaatg aatactccaa ctcttaacaa ttaactgtag ccttaagata tgtaaattct
    63361 tcatataata taaatccttt tacaattaat cataaaaaga ctgtatatac ctcatttcgc
    63421 acttatatgc acaatgtata tacagtcttt attactatta taattacatt cttatcttat
    63481 agctttagtt taaatttaaa attgcagatt aatttatttt aaatttttct atgctctcat
    63541 tgtagactat tttaatgaat cttctatgcc tttacctatt tgtgtaccca tttcttttac
    63601 tttatttgat gtattaagta aactcatatc tttctcatta tccaaaaatg cacattctac
    63661 aagtacagct ggcatatcat tatttcttaa aacatataaa taatcttttt tagggtcagt
    63721 tgatagtgtt cttgtttttg cacctctatt ttttagatta aatttctcta aaattctatt
    63781 taatatattt gtagctacag tcttagtagt tcctccatct ttatctttaa gcttataaaa
    63841 tacttctaca ccattcccag ttgtatcaga tgcattatag tgtatactcg taaataaatc
    63901 tggtctcaaa gagttagata aagcagttct atttccaaga gataaagtct tatctgtatc
    63961 tcttgtcatt attacattga atccctttga acgtaaatat tcagttgtcg caagtgaagt
    64021 atttaatgta tagtcttttt ctttaatacc ccctaatggt ttacctgttg ttccagtgtc
    64081 cgaaccacca tgtcctgggt caatcataac tgtcttaccc ccactatttc ctggttctgt
    64141 tggagttgta ttgtgtttag ataatgatga tgctatagag ttcattacct tagaagtcaa
    64201 tcctcctcct attttatata ctttattagc tgatttagct tctaaattat ctttatggta
    64261 tgcagataca tacgtctttg gtgttataag tattggagat tttaagttgg cagccaatgg
    64321 acctgctgct aaagcatcaa caagtacatc tgatttagct actaaaacag cctctaactc
    64381 atcatctgta tagaattttt ttattacgtt tgcatttgtt tcgtgtctat ctgctccata
    64441 tactctatta ttagtaacat tatctttagt tatatcattt accttattta taacatttgt
    64501 tgatatcatt tgagggccac ctataaaata agcattttgt aaatcctcac tctttaacca
    64561 tttatatgta ttgtctgtaa tactattttt atcagttaat ataattggtt gcttgtcttg
    64621 acctgctttt gctgctatag taagtgcatc cacttctccg ccattagcat ttgttatgta
    64681 tactttttcc acatcatgat ttttatctat ttcctttgct atcaataaag atgtctcata
    64741 tctattagag ccctttagac gtgtttgact agcatttaca gtcgatttaa tttgattagc
    64801 agtagtttta gaaatagcat tctcatctcc aattataatt acatccctag gatttaaacg
    64861 ctttaattct gattttacac tattaggtac attgtttttt tcaaccaaaa gtattggcgc
    64921 attatatgta gttgctagtg gagttgatat aatgccatct atacttacat ccccatttat
    64981 tataactacc ttatctgaac catttttcca gccttcttta cttattttaa ctgctgtttc
    65041 gtatctatca cttcctgtaa gttctgtaat aggtgcagaa gtttttctta aatatttatt
    65101 tacatctttt ctagaagtta tatcttcaat tatacattcc tgcgttacat tatagtcttt
    65161 taaatttaat cccatctttt ctagttcttg tttgtttaaa gttttaggat tatttttgct
    65221 gtttattgtt atcccttgta gttcccaaac atttaaatat tctgggtctt tattataatt
    65281 gatagttcct tcatatgact cagttgattg attataattt agagttgtat ccaatggcat
    65341 atcatagcat ttatacttta atgtaatgct atctgcatct ggtttttctt taaacttaac
    65401 actgactttt gatttgctac ctaaaacgat ac
//
LOCUS       DAGSWI010000004        57875 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.004, whole genome shotgun sequence.
ACCESSION   DAGSWI010000004 DAGSWI010000000
VERSION     DAGSWI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57875
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.004"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     regulatory      594..679
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            841..1362
                     /locus_tag="MJF80_000194"
     CDS             841..1362
                     /locus_tag="MJF80_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBZ0278880.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTTTDKAGLTILETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTDTEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGDELTDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            1514..3190
                     /locus_tag="MJF80_000195"
     CDS             1514..3190
                     /locus_tag="MJF80_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBZ0278881.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            3202..4410
                     /locus_tag="MJF80_000196"
     CDS             3202..4410
                     /locus_tag="MJF80_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBZ0278882.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            4425..6137
                     /locus_tag="MJF80_000197"
     CDS             4425..6137
                     /locus_tag="MJF80_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBZ0278883.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            6130..7038
                     /gene="pilO"
                     /locus_tag="MJF80_000198"
     CDS             6130..7038
                     /gene="pilO"
                     /locus_tag="MJF80_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBZ0278884.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            7065..7631
                     /locus_tag="MJF80_000199"
     CDS             7065..7631
                     /locus_tag="MJF80_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBZ0278885.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            7668..8195
                     /locus_tag="MJF80_000200"
     CDS             7668..8195
                     /locus_tag="MJF80_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBZ0278886.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            8216..9754
                     /locus_tag="MJF80_000201"
     CDS             8216..9754
                     /locus_tag="MJF80_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278887.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRDISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLEKLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            9854..10936
                     /locus_tag="MJF80_000202"
     CDS             9854..10936
                     /locus_tag="MJF80_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBZ0278888.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            10917..11699
                     /locus_tag="MJF80_000203"
     CDS             10917..11699
                     /locus_tag="MJF80_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBZ0278889.1"
                     /translation="MDIIIVNLYVFMVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            11801..12451
                     /locus_tag="MJF80_000204"
     CDS             11801..12451
                     /locus_tag="MJF80_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBZ0278890.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            12465..13025
                     /locus_tag="MJF80_000205"
     CDS             12465..13025
                     /locus_tag="MJF80_000205"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBZ0278891.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            13042..16428
                     /gene="mfd"
                     /locus_tag="MJF80_000206"
     CDS             13042..16428
                     /gene="mfd"
                     /locus_tag="MJF80_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBZ0278892.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            16459..17454
                     /locus_tag="MJF80_000207"
     CDS             16459..17454
                     /locus_tag="MJF80_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBZ0278893.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFDDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            17578..18117
                     /gene="spoVT"
                     /locus_tag="MJF80_000208"
     CDS             17578..18117
                     /gene="spoVT"
                     /locus_tag="MJF80_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBZ0278894.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            18237..19850
                     /locus_tag="MJF80_000209"
     CDS             18237..19850
                     /locus_tag="MJF80_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBZ0278895.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            19957..21405
                     /gene="mazG"
                     /locus_tag="MJF80_000210"
     CDS             19957..21405
                     /gene="mazG"
                     /locus_tag="MJF80_000210"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBZ0278896.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            21494..21772
                     /locus_tag="MJF80_000211"
     CDS             21494..21772
                     /locus_tag="MJF80_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBZ0278897.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            21844..22086
                     /locus_tag="MJF80_000212"
     CDS             21844..22086
                     /locus_tag="MJF80_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBZ0278898.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            22148..22402
                     /locus_tag="MJF80_000213"
     CDS             22148..22402
                     /locus_tag="MJF80_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBZ0278899.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            22403..22936
                     /locus_tag="MJF80_000214"
     CDS             22403..22936
                     /locus_tag="MJF80_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278900.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            22942..23226
                     /locus_tag="MJF80_000215"
     CDS             22942..23226
                     /locus_tag="MJF80_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBZ0278901.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            23339..24259
                     /locus_tag="MJF80_000216"
     CDS             23339..24259
                     /locus_tag="MJF80_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBZ0278902.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            24349..26721
                     /gene="spoIIE"
                     /locus_tag="MJF80_000217"
     CDS             24349..26721
                     /gene="spoIIE"
                     /locus_tag="MJF80_000217"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBZ0278903.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGSLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            27072..28964
                     /gene="pepF"
                     /locus_tag="MJF80_000218"
     CDS             27072..28964
                     /gene="pepF"
                     /locus_tag="MJF80_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBZ0278904.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            29078..30043
                     /gene="galU"
                     /locus_tag="MJF80_000219"
     CDS             29078..30043
                     /gene="galU"
                     /locus_tag="MJF80_000219"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBZ0278905.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            30543..32147
                     /gene="rho"
                     /locus_tag="MJF80_000220"
     CDS             30543..32147
                     /gene="rho"
                     /locus_tag="MJF80_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBZ0278906.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            32300..32500
                     /gene="rpmE"
                     /locus_tag="MJF80_000221"
     CDS             32300..32500
                     /gene="rpmE"
                     /locus_tag="MJF80_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBZ0278907.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            32645..33538
                     /locus_tag="MJF80_000222"
     CDS             32645..33538
                     /locus_tag="MJF80_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBZ0278908.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            33556..34404
                     /gene="prmC"
                     /locus_tag="MJF80_000223"
     CDS             33556..34404
                     /gene="prmC"
                     /locus_tag="MJF80_000223"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBZ0278909.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            34448..35512
                     /gene="prfA"
                     /locus_tag="MJF80_000224"
     CDS             34448..35512
                     /gene="prfA"
                     /locus_tag="MJF80_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBZ0278910.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            35641..36321
                     /locus_tag="MJF80_000225"
     CDS             35641..36321
                     /locus_tag="MJF80_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBZ0278911.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            36350..37390
                     /locus_tag="MJF80_000226"
     CDS             36350..37390
                     /locus_tag="MJF80_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBZ0278912.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            37407..37856
                     /locus_tag="MJF80_000227"
     CDS             37407..37856
                     /locus_tag="MJF80_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBZ0278913.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            37873..38325
                     /gene="rpiB"
                     /locus_tag="MJF80_000228"
     CDS             37873..38325
                     /gene="rpiB"
                     /locus_tag="MJF80_000228"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBZ0278914.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            38361..38990
                     /gene="upp"
                     /locus_tag="MJF80_000229"
     CDS             38361..38990
                     /gene="upp"
                     /locus_tag="MJF80_000229"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBZ0278915.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            39285..40100
                     /locus_tag="MJF80_000230"
     CDS             39285..40100
                     /locus_tag="MJF80_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBZ0278916.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            40326..40763
                     /locus_tag="MJF80_000231"
     CDS             40326..40763
                     /locus_tag="MJF80_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBZ0278917.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            40948..41175
                     /locus_tag="MJF80_000232"
     CDS             40948..41175
                     /locus_tag="MJF80_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBZ0278918.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            41180..41566
                     /locus_tag="MJF80_000233"
     CDS             41180..41566
                     /locus_tag="MJF80_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBZ0278919.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            41569..42273
                     /gene="atpB"
                     /locus_tag="MJF80_000234"
     CDS             41569..42273
                     /gene="atpB"
                     /locus_tag="MJF80_000234"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBZ0278920.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            42342..42602
                     /gene="atpE"
                     /locus_tag="MJF80_000235"
     CDS             42342..42602
                     /gene="atpE"
                     /locus_tag="MJF80_000235"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBZ0278921.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            42725..43222
                     /gene="atpF"
                     /locus_tag="MJF80_000236"
     CDS             42725..43222
                     /gene="atpF"
                     /locus_tag="MJF80_000236"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBZ0278922.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            43219..43764
                     /locus_tag="MJF80_000237"
     CDS             43219..43764
                     /locus_tag="MJF80_000237"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBZ0278923.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            43781..45283
                     /locus_tag="MJF80_000238"
     CDS             43781..45283
                     /locus_tag="MJF80_000238"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBZ0278924.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            45311..46189
                     /gene="atpG"
                     /locus_tag="MJF80_000239"
     CDS             45311..46189
                     /gene="atpG"
                     /locus_tag="MJF80_000239"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBZ0278925.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            46202..47596
                     /gene="atpD"
                     /locus_tag="MJF80_000240"
     CDS             46202..47596
                     /gene="atpD"
                     /locus_tag="MJF80_000240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBZ0278926.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            47599..47859
                     /gene="atpC"
                     /locus_tag="MJF80_000241"
     CDS             47599..47859
                     /gene="atpC"
                     /locus_tag="MJF80_000241"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBZ0278927.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            48321..48701
                     /locus_tag="MJF80_000242"
     CDS             48321..48701
                     /locus_tag="MJF80_000242"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBZ0278928.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRDEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            48716..49177
                     /locus_tag="MJF80_000243"
     CDS             48716..49177
                     /locus_tag="MJF80_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBZ0278929.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            49198..49788
                     /locus_tag="MJF80_000244"
     CDS             49198..49788
                     /locus_tag="MJF80_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278930.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            49803..50960
                     /gene="alr"
                     /locus_tag="MJF80_000245"
     CDS             49803..50960
                     /gene="alr"
                     /locus_tag="MJF80_000245"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBZ0278931.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            50993..51280
                     /locus_tag="MJF80_000246"
     CDS             50993..51280
                     /locus_tag="MJF80_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBZ0278932.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            51249..51632
                     /locus_tag="MJF80_000247"
     CDS             51249..51632
                     /locus_tag="MJF80_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBZ0278933.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            51803..52630
                     /locus_tag="MJF80_000248"
     CDS             51803..52630
                     /locus_tag="MJF80_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBZ0278934.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            52623..53564
                     /locus_tag="MJF80_000249"
     CDS             52623..53564
                     /locus_tag="MJF80_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBZ0278935.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            53987..55081
                     /locus_tag="MJF80_000250"
     CDS             53987..55081
                     /locus_tag="MJF80_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278936.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            55307..55723
                     /locus_tag="MJF80_000251"
     CDS             55307..55723
                     /locus_tag="MJF80_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278937.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(55905..56474)
                     /locus_tag="MJF80_000252"
     CDS             complement(55905..56474)
                     /locus_tag="MJF80_000252"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBZ0278938.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLNKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            56628..57785
                     /locus_tag="MJF80_000253"
     CDS             56628..57785
                     /locus_tag="MJF80_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBZ0278939.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
BASE COUNT        23246 a         6146 c        10805 g        17678 t
ORIGIN      
        1 tatgtaaata taaataaaga aagaggtgtt tccaatggga aggttagaag atgaacccct
       61 aataattaaa tgattcagtc acaatctaaa gtgaatataa aaatctattg aaagtttatt
      121 tataagaatt gaaaatagta tctattctat ctttagtgtt tgtgagaggg ttctggatta
      181 tataagatta ctgtaaagag taattttgca caataagcat atctgtaaat gaagagtttc
      241 gtttatataa tgattgtggc tgaatcataa ctataataga tagaatgttt ataatagatg
      301 attttataaa atactatatt gtaatagctt ttagctttca aataaatatt agcaaaggat
      361 ggaaatgaca atgataaaac taaatgataa tttataaata tctccttacg atatataata
      421 attgtaggga gatgtttctt tttttttgta tagcataatt tttctagaaa ttctgtttca
      481 atatgtaaaa agttggtata tataaatagt ataaacagtg ttgctaaaat atggtagata
      541 ttatataata taagtggata tgtatataca ttatgagtta tataatttga atttaataaa
      601 atagaaacgt tgatttatgt tctgtaatgt gggcaccttg gagcatattg agttagtggt
      661 gcaaccggct atgaaattgt atttatttat aaatactatt attttcatag ctttttttat
      721 ttaaaaaatg gttatatgtt agaaataaat ttggttagta cgtttaaata attttactta
      781 gatttttaac cagatatatt gtaagtaagt attaattaaa aaatttaagg gggaataaaa
      841 atgaagttaa aaaagaataa aaaaggtttc actttagtgg aattattggt agtaattgca
      901 attataggta tattagcagt agtggcagtt ccagctttat ttagtaatat aaacaaggct
      961 aaggtagcaa gtgttgagtc tgattatagc tcagttaaga gtgcagcatt atcttattat
     1021 tcagatacta ataagatacc agttacaaca actgataaag ctggtttaac tattttagag
     1081 acttatatgg agtctttgcc tgataaagct gatataggtg gaaaatataa attgattaaa
     1141 gttggtaata aattggtatt acagataggt acagatactg aaggagttac attaacagaa
     1201 gcacaatcag caaaattatt gagtgatata ggtaaagata aaatatatac aggtgttaca
     1261 ggagataatt ttggagacga attaacagat actacaaaaa tagataataa agctctatat
     1321 atagtactta tagataatac tgtgatggac tcaacaaaat aattaaaact atatttttat
     1381 aaagaatatt gtaaatttaa tgaaaaatat aatataaaag acaggaagaa aatcttcctg
     1441 tttttttata ctattttctt taatgttcaa ttatgaatga tgatataatt gagatagtaa
     1501 aggagtgaaa cctgtggcta aaaaagttag aataggagac aaactggtcg aaaaaggata
     1561 tataacagaa gaacagctta aatgggcact atcagaacaa aaaaacagtg gaaaaagact
     1621 aggagaattt ctagtacaag aagggcttat agatagtaac ttactaataa gtgtattaaa
     1681 agaactcttg gatatagaaa gtatattttt agaagggaca gaaatagaca ctttagctac
     1741 aaaaatggtt ccagaaaata tatgcaaaag atatactgta tttccattta aaattgatgg
     1801 aaataaaata tgtctagcca tgtcagaccc acaagataga gaagcagttc aagatgtaag
     1861 gcgtatgtct ggtaaagacg tagagatatt tatatcaagt acagaagata taaacaaagc
     1921 aataggtcat gcttatgcac attcagaaat aaataaggca atgactgagt acaacaaaaa
     1981 tagaactggt ggagtaagag aaacagtaat acttgaagaa gatgtaaatg ctgctccaat
     2041 agtacgatta gtaaataaca tactagaaaa tgctgttaga atggaagcaa gtgatataca
     2101 tatagaacaa agtgaaaatt acatgcgtgt taggtttagg atagatggaa tgttaagaga
     2161 atatatgagg atgaactcag caccatataa ggcagtaatc agccgtatca agataatgtc
     2221 agatataaat atatctgaaa agagaatacc acaggatggt agaatctatt taaaagtaga
     2281 taataaaccc atagatttta gggtttctac aatgccaact aacagggatg aaaagatagc
     2341 tatgagggtt cttgataaga gtaattttat ggttagtaaa gaagtactag gaatagacga
     2401 acatggctct aagatatatg atgaattgat aaacactcct tatggtctta tattggttgt
     2461 tggccctact ggaagtggga aaaccactac attgtattca atgttaaatc aactaaatac
     2521 agaaaataga aatctattaa caatagaaga ccctatagag tatgaattac ctggtgtaaa
     2581 tcaatctcaa ataaatgaaa aggcagggct tactttcgcc agcggactta gagcttttat
     2641 gcgtcaagac ccagatataa taatggttgg agagataaga gatactgaga cagcagaaat
     2701 agcaattaga gcttctctta caggacattt agttttaagt acattacatg caaatacagc
     2761 agttggagca atttcacgtc ttttagatat ggatgtagaa agttttttaa taacttcatc
     2821 tgttttaggt gtaatatctc aaagacttac acgaaaaata tgtgagcatt gtaaggtttc
     2881 atatgaagca gatattggag aaaaaaaggc tcttggtatt gatgtaaatg aatcagtaac
     2941 tatttataga ggaaaaggtt gtgaaaggtg taataatact ggatataaag gacgtctagg
     3001 tatatttgaa atgcttgaaa ttactccaga aataaaagag ttaatagatt catctgcaaa
     3061 tcaaagagaa attttaaaga tggctagaaa acaaggtatg gttagcttaa aagaggatat
     3121 agttaaaaaa gttttaaatg gaaagacaac agtagaagaa atgattagaa taattttgat
     3181 gactgattaa aggatgatat tatgaaaagt tttaaataca cagtaataaa agaggacgga
     3241 aaaaagcaaa ctgatgtgat tgaagccaac gattttaatg aagctagaaa tcttcttaga
     3301 aagagaaatc ttagagttat tgaaatcaaa gaaagcaaaa ataaaaatat aagattatct
     3361 agtagaaaga gaaaaaaaga cttgggagct gaccagataa gccacttttg cagacagttt
     3421 gctattatag tatcttctgg tataaatagt atttctggat tagaaacatt agctagaagg
     3481 tcaaccaaca ttacattaag agaagaaatt aatcgtatag tggcagaaat aaaagtaggt
     3541 tctactattg cagattcaat gctttcatca aaatcaaagt ttccaaagtt acttggtgct
     3601 atggtagcta caggtgaagc tacaggtaaa ttagatgaag tattaaaaag tatggcttca
     3661 ttttatgcaa gtgaacacag ggttaaacaa aagcttagaa atgctgcaac atatccatta
     3721 atagttttga tttcttcatt tataatgatt tttatattta caacatttat ggtgcctaag
     3781 atgattaatt caattacaac agtaggagca actcttccac ttataacaag aattgttatg
     3841 gggtttggta tgtttatgaa gaagttttgg ataatagtac ttttagttat tatattattt
     3901 atctatcagt ttaaaaaata tctaaaaacg tcagttggaa gggctcataa agatagagtt
     3961 ataaataaga tacctgtttt aggaaaagga ataaattgta tggtagctac acgattttca
     4021 agagcattgt atctatttgt atcaacagga tacccattag ttcaagggct tgattatatt
     4081 atagatagtg taaataacac tatggcagag aagattttag catcagctaa agatggaata
     4141 acaagaggag aaggcttagc agaaaattta gagagatata cgtattttga ctctgtgttg
     4201 gtacagatga tagctatagg ggagcagaca ggggaattag agaatataag tagacagatg
     4261 gcagaatttt atgagtatga atccgagatt tatttaaata ggatggcctc aatgatagag
     4321 cctgttttaa taattgcagt tggtattata gttgcgatac tggttgtatc aatattcatg
     4381 ccaatgctta gtatatatga tgccatgtag gagggaaagg acaaatgaac aaggtttatg
     4441 tatcatatta tggcgattct atttccatag ttgagggcag atataaacac gataaggata
     4501 agttttatgt aaaaaatttt aatgtgtttt caatagagga tattgcacaa gatttttcta
     4561 aggaaaagta tgctctattg agatacgcat tagaaaatgt taagttaaaa tcaagacatg
     4621 tagttttttg tttaaataca agagatgtaa ttttaaaacc ccaaaaactt cctaatatgg
     4681 acaaaaagga tttagacgga tttatgaagc tagagatgga cgaaatgatg gctttggagt
     4741 cagatgaata tgtattttcg tatgaagtat ctagtgaaac acctgataat tcagatggcg
     4801 atgaatcaag tttaaatctt attattggag ctatacaaaa ggaagaggta aatgcaattg
     4861 tagagataat gcatgaattt gatttagtat tagatagaat agatacatta tcaacagctt
     4921 acttaagaat gcttaaaaat ttagactaca atgatataat ggtaaccaat ataagtgaca
     4981 atagcacaat tataaatata tataagaaag atactttatt tattagtgat aatataccta
     5041 taaagataac tgctaaagat atggacatgc aattattaaa tgtagtagaa gaatcaaaag
     5101 gtcttatgaa ttactattcg tcaagaaact ttggtaaggt tacggatact atattaatat
     5161 taggaaaaag atattccaat aaattaattt atgaaacttt tagctctgca ttttcaagct
     5221 atgtttctca aggccttact gagataatgg atatatctga tatgataact ggagatatag
     5281 atgaagatga tataaattca attgtagagc ttttaggaag tatgatagaa cctaaaggga
     5341 aaagagattt tgaaaatata aactttttac caatggatat acttagaaaa cagataaaag
     5401 ctaaacaaat gaaaaacata ctgaaaattg ctccaatagt tattttgatt cttgctatac
     5461 cttatttatg tttaattttt tcaaataatt tagcaaataa ggaacttgaa aatgttaatg
     5521 gtgaaataag aaatattgaa tctgcatatt atcagattgg aaatatagaa aaaatgatac
     5581 agagtaaaag cgaagaaata aaggtatatg atatgttaat tgggaaaaag gtaaagtggg
     5641 aaaatttatt ggatgatata gataaaaata ctccaagtaa agttgaacta acaagtttat
     5701 caacaactta tcaaaatctt ggaactaata ataaagctga taattcaaaa agtaataaga
     5761 ctacaacaga taaatcaagt aacaatgaaa caaaatcaag tacgaaaagt gatagtaatg
     5821 gagataaaac agataatgat ttaaatgaag ataagactac aaatgtgtca gaagcattaa
     5881 ctggaaatag tgagactaat agtaataaca cagataaaga tgataaaact ccattgtatg
     5941 ataagatacc aaatgttata aatatagaag gaaatgctaa agattcctct tatgtaggtc
     6001 aatttttata taaattaaag ggattatact atttcaagga tgttaaatta catagcataa
     6061 aaagtaatgc agaagattct aactatacat ttagtatgac attatacctg aaagaaggtg
     6121 tattaactaa tgaataaaga tatatttaaa aaagatataa aagatatatt taaaatggaa
     6181 gttagtctaa aagggattct tataaaggtt ataattttat tagctatttt atcatatggt
     6241 tattttggac tttaccctca atatcagaaa tatattgcta caaaaaattc tgtaaaacag
     6301 gctactgaaa aattaaatat atataaagag aaaactgcac ttatgccaaa atatgaaaaa
     6361 caacttgaag aagttgaaag tgaattaaaa gaaaaaagca aaaatctttt atatgatatg
     6421 caagatggtc tttttttaat aggtttagat aaaaaaatga aggcttataa tattgactta
     6481 accagttatg acgttggaaa tgttaaagaa tacgaacatt tttatggtat atcaacaaca
     6541 ataagtgtta gaggtgatta tagaaatgtc aaaaaattaa tggcttattt agaagaacaa
     6601 aaaaatgtta ctcaaatact agattataca atgacttcat atatagaacc agaaaaagaa
     6661 gaggaagaaa caacaaacaa taaggattta attaaaaatg tgaacataaa taaagataca
     6721 aaaatatact gggtggaagg cgaaaataag tggcatttat ataaggattg tccattgata
     6781 aaagagggag agattctaaa aaatgacaaa cttttagata ggtctactaa ttatctatgt
     6841 aatacttgtg tacttagagc tgcaacagaa aaagaagcaa aaaaagaagt cgtagctcca
     6901 aaggcagaag gtgtagtaga tgctacattt aaatttataa tgtacactaa agagaatact
     6961 acaatgatac ttgatacaga taatccatct tcatggagac caggaaaata taatccattt
     7021 aagtctagtg cgaaataaag ctaagtaagc tttaaagata ggtgatgagt ttgtacaaaa
     7081 ataatgaaaa aggtcttact ttattagaag taataatagc tgtttttata ctgacaatag
     7141 ttttaagtat ttcttataaa gtgttcaatg gtataacatc agcagtaaaa aagcaacaga
     7201 ttattacaga tgctcaagta aatattaatc taattaataa gtatctaaat agagatttgg
     7261 aaaactgtaa agaactaact aaaactggtt caggtaataa ttatgaatac aatatagaga
     7321 tgccagataa tgttgtaaaa tatgaagttt ctatagaaac taaaaaaaat acagaggtat
     7381 attctgtaac gagaatccaa aacaatacaa ttgatacaga aaatgaagtt agagaagaga
     7441 taatctataa tcaaccacta gttcaaaaca ataaagaaat gaaggaaaca ccatttaaaa
     7501 tagaaaagca aactggtaaa tctatttata ctgtaagtat atactataat gaatcagtgc
     7561 aagaaagtca taaaaatagc aatttaaata acaaaactta tacatttgat gttatgtcaa
     7621 gaataggata ataagaattt attaaaaaat aagtaggttg ataaaatatg agtaaaaaga
     7681 atagtgaaag aggattttcc ttaattgaag tgttagtagc tatggctatt atgggaattg
     7741 tactatttgc attttttaat attatcaata ctaataacaa agcaaacact aaaaatgaca
     7801 cagatataac ttccttaaac tatgtccaaa gtgaaataga aaatctaaga gaaaagataa
     7861 aaagtggaga atttgatttt gatagtttag ataaactaga agatggaact gtcgtatatg
     7921 aaaaattaat agataaatca aaaaaagttg tatatgataa agttcttagt gaaggtgatg
     7981 tgagcttata tgatactcca tatgaaaaga ttacaacaat aaaagatgaa gatggtaatt
     8041 taatcgacaa aggaaatatt acaaataaga taaagactat tgtagaagat aaaagtggtc
     8101 agatatataa aatagctgta actgggaaaa gcatgaatga ctattcaagt aaaaaagagg
     8161 ttaaaattgt aactgaaata tttaaagata aataatttta cttgggggtg agattttgag
     8221 gaagtggaat aaatttaaaa gtgaaagagg ggcagcttta gttttagttt tggttgtagt
     8281 agcattgctt tctattgttg gattgatatt ttcaaatcag atagctaata gaataaaatc
     8341 tacaaagact actaatgaag gtatacaagc aaagtattta gcagagacat gtgtggaaaa
     8401 tagtattgat aaagcatatg aaaaattgta tgatgagctt gagaaaatgg ataatgagtt
     8461 taaaagtgaa aatcaagaaa aatccatatc taggtcaaaa ttaagagata tatcagatga
     8521 agattttaat aatcaagatg aggaaaatat tgaagcagaa agacttggat atatgaataa
     8581 tataaatttc tatttaaata aagcttcttc agacttggaa aaagctagta tggaattgaa
     8641 aaagctatat gatttagata tgttggacta tagagacatt gaatatgtag atgcaaatat
     8701 tatttcacat agagattcta tcttagaaat ttgcaagaat tatacttctg gagatattag
     8761 taaaataaat gaatatattc tgaaagaaga tatagactca actacattga ttgaggcaaa
     8821 gttagtgaat aatgatattc ttttaaaaat gtttttagaa gaaaataaaa ttgaaaatga
     8881 acatttaaat tcagcctttt cacatactta taaggcatta gataacattt ctttggcgat
     8941 gcaaaatatg attgagtata gacatacgtt tcatatagat gagccaaaag tagaagtatc
     9001 taatgggatt ccagattctc aacaatatta tgaactaata caaaatccta taataaatag
     9061 tatggagtat atctggaata gtaaatggga tacattagaa aaactattgg aaattttacc
     9121 aaatcaaact caaggattta attctttaag agttcatttg agaaataatg taagaaaatt
     9181 tgagaagtta agcgataata tatctagtgg taaaaaaaat acagcaaaaa atttcttaaa
     9241 gtataaagag ttattgtatg aaatatcaga ccaatgtaat caactaaaaa gtatgtctta
     9301 tgagaaaata cctgttaaat atgataacat ggctttgata acaacttttg actatataca
     9361 aaatgagctt ttagcagaga taaaatgcag attaaaagaa ttaaaaccac aagaaattga
     9421 taaaacagaa ggtattacta taaaaatacc tttttataag gctgactacg acatgacaaa
     9481 agaaggatgg cctaaattaa aagaaaatgg ttcaggggcg gagttaagct taatggtaac
     9541 tggtgataaa gatggaataa aagaagtaga agttacagat ggtaaaaaaa atattattgg
     9601 tttaggtgtt gaagaaaatt caaattcaaa gtataaggtt gacgccattg taaactttaa
     9661 tctaaatata gatacaaatg tagttggaaa ttatgatata aaagataaaa ttttgattaa
     9721 tcatgatata agttcataca aaaaagtaaa ctaaatataa gaagcaactt agtataaata
     9781 aataataaaa taattttatt atttactact aaaaaaataa gtaaactaat agaagatatg
     9841 gagagtaaat attatgtata tttatgattt attagagcaa ggtataagac taaacgcatc
     9901 agatatacac ataacagttg gaacaaatcc tgtagctaga gtaaaaggtg gatttgtaaa
     9961 actaagtgaa caaatattga catcagaagt aacaatgcaa atggcaaaag acatagcagg
    10021 ggaatctatg tttaaagtaa tagaagaaca tggagaagct gatttttcag cttctttaaa
    10081 aacaggagaa agatttaggg taaatgcata taggcaaaaa ggaaattacg caatagcaat
    10141 aaggactata acagcagaaa ttccaacatt tgaaaaatta ggccttccag agagtataaa
    10201 atcttttact gaaaagcata aaggattagt tttagttaca ggtccaacag gaagtggtaa
    10261 aagtacaaca cttgcaagta tgataaatat aataaatgaa aaacaacaaa aacatataat
    10321 tacacttgaa gacccgatag aatatgtaca tcatcataaa caaagtttag taaatcaaag
    10381 agaagtagga actgatacag aaagttttca ttcagccctt agagctattt tgcgtcagga
    10441 cccagatgta atactaatag gagagatgag agaccctgaa actgtttcaa tagcattaac
    10501 agcagcagaa acaggtcatt tagtattttc aacactacat acagtggggg cagctaagac
    10561 aattgataga atagtagaca tgtttcaacc atcccaacag caacaaataa aaacacagtt
    10621 atctacagtt tgtgaaggtg tggtatcaca gcagttactt cctacagcag atgggaaagg
    10681 cagaatagca gcaattgaat tgatgtttgc tactccagca ataaaaaacc tgataaggga
    10741 aggcaaaaca tatcaaattc caaacatgat acaaactgga gttaaagtag gtatgaaaac
    10801 tatggaccaa gatttaatgg aactttataa aaatggaaag attacaaaag atatggcact
    10861 tagtaggtgt actgaccaag agtttatgac aagaatgata ggtggagtaa actacaatgg
    10921 atattataat cgttaatcta tatgtattta tggtgggtat agtgtttgga agtttcttta
    10981 acgtctgtat ttttaggatt ccagaaggta tatctatttc tagacctcct tctcattgta
    11041 tgagttgtaa tactagacta aagccaatag acttaatacc agtgttaagc tatttaatga
    11101 gtggcagaaa gtgtagatat tgttatgaga aaatttctag tagatatgct atagtggaat
    11161 taattacagg acttttattt ttaacagtat ttatgttata tggaatatct acatcaacta
    11221 tttattattt ggttcttgtg tctttactga ttataattac atttatagat attgaccact
    11281 ttataatacc agataaaata ttggtttttg gagctatatt ttctatgatt tttaaccttt
    11341 tatttaaaga aattccagtt aaggattcaa ttctaggtgt ttttatttgt ggaggaagtg
    11401 tctggattat agttcttttg attgaatttg taataaaaaa agagtgtatg ggtggaggag
    11461 atatcaaact atttgcaatg ctaggttttt acatgggtgt taaaaatggt cttttgacag
    11521 ccttactaag cgtttatgtg ggagcagtct atggaatctg tgtaataatt ataagtagaa
    11581 taaaaggaaa ggaatataat tctgttatac catatggacc ttttatatct attggagcat
    11641 taataacaat cttatgtgga aagcaattac ttgaacttta tttttctatg gttatataga
    11701 tgatagtcaa gttaaataaa gttttaattt aaaaaaatga attaaagtat ttaataagga
    11761 caatattatt attaacccta ttactaaagt gctacaaact atggagtata taataataat
    11821 ttccttattt tttgcacatt tgataaataa atctctatgt gttttttgtg atagagaaaa
    11881 gaaaaattta tacaatacgt tttttattta cataataact gtagttttat ctgtattaat
    11941 atatctaaaa tttgggatta gtgtagaatg tgtacaatat atggttttaa ttccattttt
    12001 aataattata tccattgtag attactacac aatatatgta tatgatataa caatagttag
    12061 tggtataata gttcaaggta taattttttt ggtctttttc aatatagttc aatttgatta
    12121 tataaatcat atatttggac tattgatagg ctttattata ccttatatta ttgctaaact
    12181 aacaaaagga ttaggctatg gagatgtagg tctctatagc ttatgttgct ttgcattggg
    12241 aaataattat gggttttatg tgatagtttt atcatttatt ttagcgtttt tgttttttat
    12301 tattttatac ataactaaaa tatttaaaga tgtagatata agaaagcaaa ttccttttac
    12361 accttttatt tccttgtcaa caattttaat aatatttaca gagtataata tatttaattt
    12421 ttattataat ttgttataca aatttatata acaggaggta agaaatgtac gtagtagtag
    12481 ggttaggtaa tccaggaaag aagtatgaga agactagaca taacgttgga tttgatgtta
    12541 tagatattct tgctaaagaa tacaatatta gtgttactaa aataaagcat aaagctttga
    12601 ttggtgaagg gagagtaggt actgaaaaag tattattagt taaaccacaa acttatatga
    12661 atttaagtgg agaaacatta atagatatat ataaatatta taaggttgat ttaagtaata
    12721 tagtagtagt atatgatgat atagatttag aagttggaaa gatacgtata agaaaaaaag
    12781 gaagtggagg aactcataat ggcatgaaat ctataactaa gtgcctaggt tcaaatgact
    12841 ttccaagagt gagagtaggt gtatctaaac cagaagctgg tcaagactta gcagactttg
    12901 tactttctag atttagaaaa gaagaatctg ataacataaa tgaagcatta gaaaaagcgg
    12961 cagatgctat tgactcaata ataagagaaa atatagatat gtctatgaac aaatataatg
    13021 gatagtgaga gggagattta aatgaatgat gtttttttat atcctttgca aaattcaaaa
    13081 gagtataaag atataataaa ctgtataaaa aatacaaaag gctcattatt ggttaatgga
    13141 cttttaccag tacaaaaacc tcatatatcg tattctatat ttaatgattt atcacgacaa
    13201 atgattttta taacaagtag tgatttagaa gcaaagaaag tatacgaaga tttaagtttt
    13261 tatatggaag ataaagttga atatttaggt tttcaagata tttattttta tcacttggat
    13321 gcaaaggaca gaaacgaaga agcgaagaaa ttaaaagtat tgttaaaact agcaaataaa
    13381 gaaaaaatca ttttagtaac ttctattgag gcagttttaa gaaaatacat accaaaacaa
    13441 gttttgctcg atagtgtatc tcattataag gtgggagata gcttagactt agaaaagtta
    13501 actgagaaat tagtttcatt gggatatgaa agagtttcaa aaatagaggg gtttggtcaa
    13561 tttagtataa gaggtggaat tattgatgta ttttcacttg aatatacaaa tccaattcgt
    13621 atggaattat ttgatgatga gatagattcc atacgaactt ttgacgtata ttcacaaaaa
    13681 tcaattgata aattgcaaca attctctata actccatcta gggaatttat ttatcccgaa
    13741 aaaactacag atgcattagt taaattaaaa aaagaaacaa ctaaaaatac tgatgaaggt
    13801 acatttcaaa atatagatta tatatcaagt aagacttatt ttgaaggtgt agagaactat
    13861 atagattata tttatccaga agaaaacaaa agtatattta cttatttagc agatgatgca
    13921 atagtattta ttaatgatat aacgagactt aaagaaagat gcgaaaatta tataaatgag
    13981 tttagagaaa attataaatt aaatcttgaa agaggtctgg ctattaaaaa ccaaggtaaa
    14041 ttgttatatc actatacaga tttagagtac ttagtaaaag acaagtctgt ggtattaaac
    14101 tcgttattgc caaagtctat aaataacttt tctataaaat ctataataaa ttttgaaagt
    14161 agagaagttc ctacatttaa tggaaaggta gatttgcttg tagaagaatt aaatcggctg
    14221 aaatataatg gatacaaaat aattttagca acaaatacct tagaaagagc aaataaatta
    14281 ggaaaagatt tattggacaa aggcttagaa acgactattt ctaaagatag agatattgaa
    14341 attaaatctt cacaagttat aattgtacct gcacacataa acagtggttt tcagtataaa
    14401 tctataaagt ttgttgttat aacagataac gaaatgatag gtgtttataa aagagcgtct
    14461 aagactagca ataaaaaagt taaaaaagga aagaaaatag aatccttttt agatttaagt
    14521 gttggagact atgtagttca tgaaaatagt ggtgtaggta gatacacagg gattgaacaa
    14581 attacagtca atgctattaa aaaagattat atgaaaatag tatatcaagg tggagataat
    14641 ctatatgtgc caatagacca gatggacaaa gtacaaaaat atattggtgc agaagtagaa
    14701 aaagttaagt taaataagct tggaacaaat gagtggacga aagcaaaagc aaaagttaaa
    14761 aaagaaattg aagatatgac aaaagacttg attgagcttt atgcaaaacg tgaaaaaata
    14821 caagggtata agttttcaaa agatacacct tggcaggcag agtttgaatc gttgtttcca
    14881 tatcaagaaa cagaagacca attaaaggct atagaagaaa ctaaaaaaga tatggaatca
    14941 agtaaggtaa tggataggct agtatgtgga gatgttggtt atggaaaaac agaggtagct
    15001 attagaagta tttttaaagc ttgtatggac caaaaacaag ttgctgtatt ggttccaaca
    15061 acaatattgg cccaacaaca ttataataca ttcaaagaga ggtttgaaaa ttatcctttg
    15121 agagtagaag ttttaagtag gtttaaaaca ccaaaacaac aaaaacagat tattgaagat
    15181 gctaaaaaag gattagtaga tattttaatt ggaacacata gaataatatc caaagatata
    15241 aatttaccaa acttaggtct tgtagtcatt gatgaagaac aaagatttgg agtaaaacat
    15301 aaagaagctt taaagaaaat taaatctaca gtagatgtac ttacactttc agcaactcct
    15361 ataccaagaa cattacatat gtctttaagt ggaataagag acatgagtgt tatagaagaa
    15421 cctccacaag aaagacatcc tgtaataaca tatgttacag agagtaagga aagcgtaatt
    15481 caagatgaga tagagagaga attaagtaga ggtggacagg tattttttgt atacaataga
    15541 gttgaacaca ttgaagaaat ggcaagtatg attcaaaaac tcgtaccaga tgctagagtg
    15601 gctgttgcac atggaagaat gacttctaaa tcattagaaa atataatcct aggattttta
    15661 aataaagatt atgatgtact agtctgtact acaataattg aaactggtat ggacatatca
    15721 aatgcaaata caatgattat atatgatgca gataaaatgg gattggctca actttaccaa
    15781 ttgagaggta gggttggaag aagttcaaga caaggatatg catatctttt gtatgaaaaa
    15841 gataaaactt taagtgagat agcggagaag agattaaaag ctatcagaga atttacagag
    15901 tttggttctg gttttaaaat agcaatgaga gaccttgaaa taagaggagc aggaaatatt
    15961 ttaggttctc aacaacatgg tcatatggca gttataggtt atgatttgta cgttaaaatg
    16021 cttaatgatg caattaaaaa agtcaaaggt gaacctattg tggaggaaat tgatgttgaa
    16081 atagatttaa gtgtaaatgc atacattcca gataattata tcaaagatga gttgattaaa
    16141 attgaaatgt ataagaaaat agcttctatt gaaaacaaag aagacatgtt ggatatacaa
    16201 gaagaacttg aggatagatt ctctgatata cctaaaccag tccagacact tcttacaata
    16261 gcttatataa aatcgttatg taagatatta aaaatagaaa agataagaca attaaaagat
    16321 gaaatattat tagttccaat aacaaagtat agaactaaac agaaaatagg ttataatata
    16381 gttacagaat tagaagaatt attagaaaag atgtgcaagg taaaataact tgtaaattta
    16441 agaagggatg ttttaaattt gaaaaaagta ataacattgg taatagctat gattttggta
    16501 gtatctgtga ctgcttgcag ttctagtaaa ggagaaactg tggcaacagt tgaaggtaca
    16561 aagatatcat cagatgaatt taaaaaaact atagctcttt ataaagattc tatggaacaa
    16621 acttatggaa aagatatatg ggataaggaa gttgaaaagg gtgtaaaata caaggacaag
    16681 tttaaagatt taattcttga ccaacttata actacagaag taatatattc tcaagctaaa
    16741 aaagataact tacttcctaa aaaagaggat gttgaaaaaa gttttaaaga attaaaagac
    16801 gctatgggta aagatgaaaa atataaagaa caacttaaga aattaggtat agatgatgag
    16861 tttttaagag accaacaaga gaaagattta gcaatgcaaa actatcaaag taactttgct
    16921 aaaaagacta aaatatcaga tgaagaaatg aaaaaatact atgatacaca taaagatgaa
    16981 tttaaaaaag atgaggtaga agcttctcat attttattga aaacagtaga tgataataac
    17041 aagccacttt cagataagga aaaagctgaa gctaagaaaa aggctgaaga ggctcttaaa
    17101 gaagttaaat caggtgagga ttttgcaaaa gtagcaaaaa aatattcaca agatacttca
    17161 gcaagtgatg gtggtaaatt aggattcttt tcaaggggtc aaatggttgc tgaatttgaa
    17221 gatgctgctt tttctatgaa aaagggtgaa gtatctgatt tagttgaaac tcaatatgga
    17281 taccacataa ttaaagtgac agacagaata aatgagcaaa catcatttga cgatgctaaa
    17341 gaaactataa aagaccaact gttgaaaaat aaatatcaag aacaaataga aaaattaact
    17401 aaagaagcta aggtagaaaa agatgaaaaa gtaataaata aaatcacaat ctaagactta
    17461 aaaaaagagt aatatgctta aaaaagctat atctggataa gatatagctt tttttgtata
    17521 tttttcttaa caatggtaaa aataaactta aagtaattta tggagggatt aaaaaatatg
    17581 aaagcaacag gtatagttag aagaatagat gatcttggaa gggtagttat cccaaaagag
    17641 ataagaaaga cattaagaat tagagaagga gaccctttag agatattcac agcaaaagat
    17701 ggagaagtca tacttaaaaa atactctcca ataggtgaat taaatgaatt ttcgcaagaa
    17761 tatgctgaaa cattagctga agtactagga tatggagtgt tgataacaga cttagattct
    17821 ataatagctg tatctaagct tcctaaaaaa gattacaaag aaaaaagtat aagtgatgag
    17881 ttagaagcaa taataagtga aagaacagta aaatattcaa aagataaaaa cattttacca
    17941 ttattcaagg aagaccaaaa agaatatact agtcaagtta tcatgcctat aattagtgca
    18001 tctggtgact gtataggttc aattgtagtt gtttcaaagg ataaagatac aattgcagaa
    18061 agtgaagaaa aatctttaaa aattgctgct aactttttag gaaaacaagt tcaataattt
    18121 tatcataaag accttttaca tgaaggtctt ttgaagtttt tgtgtaaaat attgaatata
    18181 gcattaaaaa tatggtatat taaagtttgt tatataaaca gtatggaggt tatgttatga
    18241 acaataacaa aaataaagac tcctttctaa aaggagccct tatacttggg ctagcaggtg
    18301 taatcgtaaa aataatgggt ggatttttta ggattcccct tggaaacatg attggttcag
    18361 aaggaatggg atactatcag gctgcttatc ctgtgtatac tctattttta acattagcta
    18421 ctgctggatt tccaacagct gtggcaaaat tggtttcgga aaaagttgca attggaaatt
    18481 ttaaaggtgc aaataagata tttaaggtat cacatacagt tttattcatt accggtataa
    18541 tatcattttg tatattattt tttggagccg actacatagt aacaaacgta atgaaaaatc
    18601 caggtgcgtt atactctatg aaagccattg ctccagcatt actatttgtt cctgcaatgt
    18661 ctgcatatag aggatatttt caagggagac aagatatgac taagattgct gtgtctcaag
    18721 ttgcagagca attttttaga gttgtattag gtcttacttt ggcctacttg cttatgaaga
    18781 gtctaggtca gacttttggt gcagcaggtg ctatatcagg ggctacaata ggttcaatag
    18841 catctatgct ttatttggtg tttgcttata tgctagggag aaaagagcgt agagcagaga
    18901 tagatgcaag ccaaagattt aaagatgaaa gagtgtctta tatttttaaa aagcttttaa
    18961 ctgttgccat tcctataaca ataggagcat cagtaatgcc acttgtaaat atgatagata
    19021 atgttattgt aataagaaga cttatggaag caggatttac atacaaagtg gcaaattcaa
    19081 tgtttggtca attaacaggt atggcaatgg ccacaataaa tttaccagca gtcataacaa
    19141 ctgcaatgag tatgagctta gttccagcaa tttctaaagc atatgcatta ggaaataagt
    19201 caaaagctag aaaagatact aagagtgcag ttaaggtaac tttacttata gtattgcctt
    19261 gtgcgtttgg tatggcttct ttagcaatac caataatggg tcttttattt ccacatgaac
    19321 catcttctgt aggaactatt ttgtttactc ttactccatg tgtattattt ttaggattaa
    19381 tacagacatt gactggtata attcagggaa tgggaaaacc aatagtacct gtaattgctt
    19441 tatgtgttgg tatgctatgt aaaattgtaa taagttatac tttaacaggt ataccagata
    19501 ttaatgtact gggttcagca tttggtacag ttacagcata ctttgtggca gccatgatta
    19561 acttactata tgttaaaaaa cacatgaatg taaacttttc taaaaaagaa tttataataa
    19621 agccatttat aacagttatg acaatgttta taatggttaa gttgagttat ggtgctttag
    19681 ttggattctt aggaaacagc atatctacaa taattgcaat atgtgttggt ggaattgtat
    19741 atgtagtagt aatacttgga ataggtggaa ttaaaaaaga agagattcta actatgccaa
    19801 aaggtgataa attatataaa ttgttaaaaa aagttaaact tataagataa tataggaata
    19861 tatatgcaaa taaaggaaat actatacaaa tagtaaatct ccttatttgt ataataatat
    19921 aaaaataaag tgaaatttgc taataggagt gatgttatgg gtaagataag tattgtaggt
    19981 cttggaccag gagactattc tcttataagc caaggtgtgt tagaatcttt aataaatagc
    20041 cctaaagttt tttttagaac agagaaacat ccaataattg ataaacttaa agaaactata
    20101 caatacactt ctttagacta cttttatgaa aaagaggaaa attttgataa tgtttatttg
    20161 aaaatatctg aatttatcat agaagaatca gcaaagggtg acctagtgta tgctgttcct
    20221 ggtcatccaa gagttgcaga gaagactgta agtataatag agagattagc caatgaaaag
    20281 cacatagcag tagaaatatt gccttctatg agctttgtag acgctatgtt taactatttg
    20341 gctatagacc catctgaggg atttaaactg gtagatgctt ttgaaataga aaattcatat
    20401 atagacttgg atacaagcat gataataact caggtatatg ataaatttat agcatctaat
    20461 gtaaagttaa aacttatgga acattatgac tataatcaag aagtatgtat agtaaatgga
    20521 gcaggtgtca aagatttaga aagtaaaaag tttataaagc tttgtgaatt agacagaaat
    20581 gaaaactttt ttgactattt aactagctta tatataccca aaagttctaa aaagatgtat
    20641 aatacagtac atgacttaga aataatagtt aatacactta gaagtccatc tggatgtgaa
    20701 tgggataaaa aacaaacaca tcaatcatta aaaaattctg ttatagagga agcatatgaa
    20761 ctttgtaatg caattgataa taatgatata gatgaaatgg tagaagagtt aggtgatgtt
    20821 cttttacagg ttatattcca ttgtcagata ggaaatgagg agggctactt tgatttaaaa
    20881 gaagtagtaa atggcatatg taaaaaatta attcatagac atcctcatat atttagtggt
    20941 gcaaaattag atatgtctaa gtttgagaaa acttgggaag aattaaagaa agaagagaaa
    21001 ggcgaaagta ctataacaga tggccttaga aggataccaa aacatttacc agctcttatg
    21061 aaggctagta aagttcaaca taaagcagca cttgtcggct ttgattggga caatgtagaa
    21121 gatgtattta aaaaaattga agaagaatat caggaattac ttgacgaaca cagggtcggg
    21181 aatataaaat acataaagga agagctaggc gatttattat tttcgatagt aaatctagct
    21241 agatttttag acatagactc aggagaagcc ttgaactgta ctacagaaaa attcataaat
    21301 agatttgact ttatagaaaa aagtgcaatg aacttgaata aaaaattaga agacatgaca
    21361 cttgaagaga tggatgagtt ttggaatcag gctaaaaaac aataaaattg tggaaggaaa
    21421 tttgtcaaaa atctagaact tttatcacaa agatagaagt ctatattata aacattttta
    21481 ggaggtaaat atcgtgaata aagctgaatt agtatcaaag atggcagaaa aaagtggatt
    21541 aacaaagaag gaagcagaag ctgcgttaaa cgcatttatg agttctgttc aagatgcact
    21601 agtaaataat gaaaaagttc aattagttgg atttggaaca tttgagacaa gagaaagagc
    21661 tgctagacaa ggaagaaatc caagagatcc agagcaagtt atagatatac cagcttctaa
    21721 agcaccagtt ttcaaagctg gaaaaggatt aaaggatata ataaatggat aattagaaat
    21781 cttgtgaaaa agtgtggata aatttgccac actttttcta tagaaccaag gaaggtgata
    21841 attatgagat tagataaatt tttaaaagta tctaggatta taaaaagaag aactgttgct
    21901 aaagatgcat gtgataaagg gattgtcact ataaatggaa aagttgctaa atcttcttca
    21961 gaggtgaata ttggagatac attagaaata caatttggag agaagaagat gaagtttaag
    22021 ataaatgaag taaaagagca tgtattaaaa aatgatgcaa aagaaatgta cgaaatttta
    22081 gaataaaatt aatagtcctc tagtatatta atagtagtat tattgaaata tttactgggg
    22141 ggatattatg gaacagaaca taactttgaa agatagatca aagctggtta tttcgggagt
    22201 cgagcacata tactctttta atgacaaaag agtagagctc aaaacttctg taggcgagat
    22261 ggtaattgaa ggcgaaaacc tagatatgag caagctaagt atagacgaaa atataattag
    22321 catagatgga actataaatt ctatggtata tgcaaagcct aaaaagccac aagagagttt
    22381 cttgaaaaag gtatttagat agatgatacc ttttactcaa gatgtaagca ttttttatgc
    22441 tactatttat ggtggaatac tcataggggt gttgtttgat ttttatagag ggctaagagg
    22501 gaattttaaa ttcatcaatt attttgctat aatctttgat gtattgtttt ggtttctagc
    22561 tacggttata atattcgtaa caataaacct tacagaattt tttgatttaa gatattatca
    22621 ttttgtggct ttgtttattg gttttatttt atactataac accataagta aaattgtttt
    22681 atcaattatc aataaaataa ttcgttttgt gagaaattct tttaaaaaag ttacacatta
    22741 catagtcagt tttttaaata atttgtatta cgttataatc tattcactac acctactatt
    22801 tgatattata ttttatatac caaacatatt tatagcaaca agaaaaagta taaaaagaaa
    22861 atcaaataaa aaattaaaga acaagaaaaa atcaaaacct aagaagaaaa aaaataaaac
    22921 taaaaaaagg gtgtaggaaa aatgaactta agaaaaaaat tttcaggaca gattattgtt
    22981 atcagtttat ttttaggaat atcaattttt agtatgatga caggatttgt atttgagtat
    23041 acaaaagcta aggaatataa aaaagaaata gcaagtctaa ataaacaatt aaaaaagact
    23101 gaaatacaaa taaacagctt aaagaaagat gaaaaatcat atgaaggaga cttagaggat
    23161 atagcaagaa aaagattgaa tatggtaaaa ccaaatgaaa cagtttatgt agacataaat
    23221 cgttgattaa aacttgctaa taagtttaca gatgtattga ttttaaaatt taagatttaa
    23281 taaaaaagtc attcagttaa ggatggcttt ttgtttttat tgaggaggga atttcataat
    23341 gaaagtagga gctatagata ttgggacaaa ctcaatgaga ttattgatag cagattatag
    23401 agatgggaat tttataaaca gaaaaaaatt tgttaataca actagaattg gttatggtgt
    23461 ggatagtaaa ggatatataa aagaagatgc tatcaaaaga aatataaaag cattaaaaga
    23521 attttctgat aagtgtaatg atgaaaagtg tgaaaaaatt tattgtatgg gaacatctgc
    23581 tcttagagac agtaaaaatg gagattactt tgttaaatta gctaaagaag aaactggaat
    23641 agatgttgat atagttacgg gaatagaaga atcaaatttg ggctttaagg gtgttttaga
    23701 aggaatacaa ggtgatgata ctattattgt tatagatatt ggaggaggtt caacagaatt
    23761 tattgtaggt gattcagaag gaattatatt taataaaagt gagaatgtag gtgctcttag
    23821 gatgacagag aggtttttga agcacgatat tattgaagat gaagagtttg aagctatgag
    23881 ttcatttata tatggtgaga ttgacaaaac gttaaatcat ataaaagaca gaggattaaa
    23941 aactcttata ggtataggag gtacgattac atcattatca gcaatgaatc aagaattaga
    24001 gatttactct atggaaaaaa tacataatag taaaatacac aaaaaagaaa tagaagaaat
    24061 tctacaaaaa ttaataaaga tgacattaag tgataaaaag aacttaaaag ggctacaaga
    24121 aaaacgtgcg gatattataa ctgctggtgt aaaaatattg aatataataa tggaaaaatt
    24181 agaaatagaa gaaataaccg taagtgaata cgataatttg gaaggcttaa tatgtcaaaa
    24241 gtcaaaaaaa atgtattaat attttttgat ttttaaaacc gattatgaca aaaaaaatta
    24301 ctcctctttg ttacaattct taatataaac aaaagaggag gtttttttat gcaaagaagt
    24361 gtagcagttg tgaagaataa gactgtaaaa ccaaatgtca taaaattaat aagcggatat
    24421 attgatttta ctacggcggc attcgctgtg atgggatttt tacttagtag gtctattata
    24481 gtagactctg tagctccttt aggaattgcc ttttttatat gtgcatcaaa gattgataag
    24541 tacaagatac ctgtattcat atcaacatta ttaggaatat tgttatcatc taatgatttt
    24601 ggaaatgtag caaaatatgc tgtgtgcctt acaatattta tggctatgaa taaaaaatta
    24661 aaattatcag attcaattgc taaggttgca tttataggtg caataatact acttccaatc
    24721 tccatgggac aagctttctt ttcaaatagg tatgtatatg gtcttcttat agcaggagta
    24781 gaagcaataa taatgtttat atctatatat gtgttttctt atgggatagg tttaatggcc
    24841 aatgtcaaca ataggatgtc tgtacggaca gaagaagcca tatcaataag tttactgcta
    24901 acctttagca taatgggaat aggagatata tctctgtttg gaatatcaat aaggaccatt
    24961 ttatcgaccg tattgatatt gctgacctca atagtaggtg gagctaccat gggagcatct
    25021 agtggtgtaa ttattggtgt agcatccatt ttaaataata tgacatcagc tgtttatatg
    25081 gggatatatt ctttttcagg attaataagt ggagcattta ataaaataaa taaatatttc
    25141 tgtatactag gatatattct aagttggaca ataatatacc tatatacttc tggtattact
    25201 tctaatatga tgcaattaag agatatacta ttaggtagtt tgatagtact agtattgcca
    25261 gagcgattat ttaataagat tgaaaaatta ataaaaagta atgtggcttc aaatgaaatc
    25321 gtatacgatt atattatgag aagcaaaaac ctaactaata gcagattgaa cagtatttat
    25381 aaaacttatg atgaccttgc agatacattt gataagataa gagaaaaaga caaagtgcta
    25441 gaccaaagag acatagctaa tgtaatagat atgattcata atgatgaatg taaaagttgt
    25501 agtatgagaa gaatgtgctg ggagtcaaga tttaatcata catacactat gatatataac
    25561 attcttgaaa aaattgagga gaaaggggag ttaagtctaa acgatatacc taaaaatttt
    25621 agaaaggaat gtatgaaacc agagtcaata gtgaaaatat caaatcacta ttacaaaatg
    25681 tttgtattag attatgattg gagtgtgaag ttttcagaaa gtagaaagtt aatagcaaat
    25741 cagataagaa gtatttcaaa atccattaaa tccttatctc aagatttaga aggcgacata
    25801 atgctagata tagaaaagga aaaaaatata tatgaacagt tagaaagata tgatataaca
    25861 gttgataaag taagttactt aacaaaaagt aacagtgagt ttgaaatatc aatagaaaag
    25921 aaaacttgcc atgatggatg tatgtgtgaa gataaaattg taaatataat ttcagaccta
    25981 gtaggggaaa atatgtctgt tagaaaaata ggatgtcact gtttaggagg taaatgcaag
    26041 gcaacattcg ttaaatctca aaaatataag gctgttacag aagtttcagc aatgtcaagg
    26101 gatgggcata tcctttgtgg agacaattac acatacatgg agataaatga tggtaaatac
    26161 atgatggcta taagtgatgg tatgggaaaa ggtaaaaaag catatgaaga atcttctgcc
    26221 actattgata tactggaaaa aatgatagat gcaaaaatta aagatgagat agtcattgat
    26281 actataaata atatgttgtt gttaaagtct tcagaagaga tgttttcaac tctggatttg
    26341 ggaattttag atttaaaacg aggttgttta gaaactataa agatgggagc gtgttctact
    26401 tatataaaaa gagaggatgg agaagttgac ttaatatcgt catcatcatt acctgtgggt
    26461 atattatcag atgtaaaaat tgatagaaaa aatgtaaaag ttaaggaagg agactatgtt
    26521 ataatggttt ctgatggaat tgtagatgca ggaagaaata acaatttagg agacaattgg
    26581 ttaatatact tcttaaagaa tatagagact acaaatccaa aggaaatttc aaatttaata
    26641 ttggatagag cattagagtt acaagcgtta caaattgaag atgacatgac tgttttagtt
    26701 acgaaaatat gtacaaaata aggtttacaa gattatgtgt ataagatgtg gataacttgt
    26761 gtataatatg ttaatagact tttgaaaaaa gtatagttat taacaaaaat aacatataaa
    26821 tcaacaaaat ttgttgataa gtatggcaga aatgttaata aaatgtaaaa aaagaaaaaa
    26881 caaaagctat tatcaaaaga gattcatgtt gaaataacta aatttctaag taaagaaaac
    26941 ctgtgaatat gttgattatt atgtggataa ctattaattc gatatttatt tatatagtat
    27001 gaataattga ataatatatc taaaaaaagc catcctatta ttaagtaaac ttattgaaag
    27061 gatggctttt tatgaataga aaattgtgca aagcaataat acctttattt atttctattt
    27121 tttgcatgtt aataataagt ataaaagctg accaaggata tcaaaagaat aatgacagag
    27181 caattactgt catgaataat aatgatactg aagatgatgc tgaaaaaaaa caagaaatag
    27241 actatgaaaa aacatgttgg aatttgcaat ctttgtttaa aagtgatgac caatggaaaa
    27301 aagaacttaa gagttttgat aaagatacta aagagttgaa aaattacata ggtaaagtaa
    27361 caaaatcgcc aacacatttg tcttttgcct taaacattaa agaaaaactt gatataagac
    27421 taaataaact ttctgcctat gtaaaattaa aacaagatac taataaaaat tcgtataagt
    27481 acttagatat gaatgatagt atgagtaaat cctatgggaa ttatatgggg atttgttcag
    27541 atttagagtt agaaatacta aaattatcaa acaaagatta caagaaaatt atttcaaata
    27601 aaaatataaa taggaaatat ggtgcatatt taagagatat aagaaggaat aaagtacatt
    27661 atttggatga taaatcagaa gatattctaa gcaaagtatc ttcaataagt agtcttccaa
    27721 gccaagtgta tgaattattt agaaatatgg atagaaaaac taatttgaca ccagctcaat
    27781 atgcgagtga attagagtca tcagatagag aaaatagaaa aaaagcttat caagatgaac
    27841 ttataactta caatgataat ataaatacaa ttgcaggttt aattactggt caagttaaaa
    27901 agaatatttt ttattctcag gaaagaggtt atgagtcttc tttagaaatg tacctggaat
    27961 ctgataatgt agatgaaaaa gtatacaata atttgataag gactgtaaat agtaatatgg
    28021 acagtctaca caaatatata gatttaagaa aaaaagtatt aaaattagat aaggttcatt
    28081 catatgatat gtttgtgcct atggttaagg ctgttgataa taatattaat tatgaaaagg
    28141 cacaaagtat agtatattca gcgttgtcac ctttaggaaa agaatacaat gatgtagtat
    28201 ataaagcttt caatgaaaaa tggatagatg tttattcaca tgaaaataaa gtaagtggtg
    28261 gatactgctt atctgtttat gaaaatcatc catatatact tttaaattat aataattcat
    28321 taggttcagt ttcgacttta tcacatgagc ttggacatgc tgtatatgaa tatttaagtt
    28381 ctaagaatca gaattatttt aattcaaatc cgtctatatt tactcacgaa gttgcatcta
    28441 caacgaatga agcattactt tacgagatgc ttataaaaga agctaaaggc aatgatgaaa
    28501 aagcttatta tataacacag tatttagact tgataaaaga tactctttat acacaaacta
    28561 tgtatgctga atttgaaaaa actatacacg agttagtaga aaaaggaaaa agtgtaaacg
    28621 cattagtatt aaatgatgtt tggggacaac ttcttaaaaa atattatgga caaagctatg
    28681 aattagacca attatctaag gtcggatggg ctagaatccc acatttttat aatagtttct
    28741 atgtctataa atatgctaca ggttgtagtg caggagtaag ttttgcccaa gacatcctaa
    28801 agaatggttc agataactat atgagctttc ttaaaaaggg aagttcagat tatccaataa
    28861 acttgttaaa atcaagtggc ataaatttaa ctaatacaaa acccatagaa aatacaatca
    28921 aaaaatttga tagtttagta gcagaacttg aaaagctatt ataaacatta ttgtaatcca
    28981 catggtaaat atatcgaatt aatgataaaa tatactaaaa gtttaaagtc ctatcaagat
    29041 aggacttttt atttattaat tagtgggaga tgattctatg caagttaaag ttaaaaaggc
    29101 tgtaatacca gcagcaggtt taggaactag atttttgcca gctacaaagg cacaaccaaa
    29161 agagatgtta ccaatagttg ataagccaac tctacaatac ataatagaag aagctgtggc
    29221 ctcaggaata gaggaaatac taataataac aggtagaaat aaaaaatcta ttgaagacca
    29281 ctttgataaa tctgtagaat tggaacttga tttagagaaa aaaggtaaga aagaattgtt
    29341 ggaggtagta caaaacatat ctaacatgat taacatacat tatataagac aaaaagagcc
    29401 aaaagggttg ggagatgcaa tttattgtgc gaggcatttt ataggtgatg agccgtttgc
    29461 agtaatgtta ggggatgata ttgtagataa tgatgtacct tgtctaaaac aactaacaga
    29521 tgcatatgag gaatatagaa ctacaatatt gggtgttcaa aaggtaaatc aagaagatac
    29581 taataaatat gggataatag aagcaaaaaa tatagaaggt agagtttaca aggtaaaaga
    29641 catggtagaa aagccagagt caggaaaagc tccttcaaat attgctattt taggtagata
    29701 tataataact cctgaaatct ttgatattct taaggattta ccaccaggta aaggtggaga
    29761 ggtacaactt acagatgctt taaaaatatt atctaagaaa gaagctatgt atgcatacaa
    29821 ttttgaaggc aagagatatg atgttgggga caagctagga tttttagaag ctactgttga
    29881 ctttgctcta aagaaagaag accttaagga agattttata aaatatttga aacatgtatg
    29941 tagtgaattt gataaaaata acaatgtgtt atacaataat acagatgaaa aaatagattt
    30001 aattgaagtc aatgaagcta gagaacttga aattcaaaaa taattaatga aaggtgatgt
    30061 atggtggcaa aatcaagtca gaaaaaaatt aatagaataa tagctgttgg gattgtggga
    30121 atcctcgtaa taacgagtat aacagttgtt ttgagtatga ttgcaactat gtaataagat
    30181 aaatgacagc agttgatata cataaatata aaaaccatat ataacttgat gtagtcaaaa
    30241 acttacaata ttttagttat atatggtttt tagaatttat aaatgtaaaa aaaaattttt
    30301 ataatagttg catattttaa aggaaaatat tattataaga tataaatata gtgataaatt
    30361 tataaaaacg tgctaagatt ggacttgaaa tacagtaata tagtaggtaa taatgtttta
    30421 aattttattt attgaccata atatataaat aatgatatta ttagacttaa ctgtttaaat
    30481 aaaacttttc tttttcatgc cttcaatttc caaaataagt atgtatataa atgaatatat
    30541 agatgaggag gatttttttg attttagatg ataaagtatt agaaggatta ggtagtaaga
    30601 ccttagttga gctacgagag atagctaaag aattaaaaat aaaatcaatt actacatata
    30661 agaaaaatga gttaattgag attataaatt ccaaaagtaa aaatgaggaa aaagtaaacg
    30721 aagagactaa agaaaaaaat gaagatatta aagaagacat aaagaataga aaagaaaata
    30781 tagagcgtga aagtatagaa aaagaagttg aatataagag ccctaccaaa tcatacaaca
    30841 agaaagaaat agatacccaa aatatttccc aaaaccaaga acatagaaat caaagaaatg
    30901 actataataa gacaacgagt gtaaatgata gcaataagtc atcaaatata tctaatagaa
    30961 tggtcagaaa taataataaa aactattata tgcctaaaca agttgatgaa tctaagatag
    31021 tagatgaatt taacacatca aaagaggatg aagtagtagg tgtacttgaa attctgccag
    31081 atggatttgg ttttttaaga ggttcaaatt atttgtctac tgaaggagat gtttacgttt
    31141 caccatctca aataagaaga tttaatatga aaacaggaga caagattaag ggaataacta
    31201 gacatccaaa aagtggagag aaatttagag cacttttata tgttcaaaaa ataaatgatg
    31261 aaaaccctga cacagctata caaagaaatg cgtttgagac attaacacca atatttccag
    31321 aagaacgact tacactggaa actaatagaa atgaaatagc tacaagaatt atagatttaa
    31381 tttcacctat aggaaaagga caaagaggat tgatagtagc acctccaaaa gctgggaaaa
    31441 cagttctttt aaaaagcgtt gcaaatagca tagctaaaaa tcatccaaat gttgaactta
    31501 tagtactcct tatagatgaa agaccagagg aagttactga tatgaaagaa tctattgaag
    31561 gagacgttat atattctact tttgaccaag tatcaagtca tcatgtcaag gttgcagaga
    31621 tggtgttaaa tagagctcaa agattagttg aacatggtaa agatgtagta attttactag
    31681 atagtataac tagattggca agggcatata accttacaat atctcctaca ggtagaactt
    31741 tatctggagg tattgaccct ggtgcacttc atggacctaa aaagttcttt ggtgcagcaa
    31801 ggaatattag acagggtggt tctttgacaa tattaggaac agcattggtt gaaactggtt
    31861 ctaggatgga tgatgtaata tttgaggaat ttaaaggtac aggaaatatg gagctacatt
    31921 tagatagaaa attagcagaa aaaagaatat tccctgcaat agatatttac aagtctggta
    31981 caagaagaga tgatttattg cttgatgatg aagaaaagac tgctctttgg agattaagaa
    32041 gagaaatgag taataattca gtaatggaaa ttacagataa agtcattgaa cttataaaga
    32101 gaactaagga caataaagag tttgtaaaat caataaagaa tttgtaaaat caataataca
    32161 aatgcaaata tggattatat aaactaatag aaaaatcact tgaatggcat aataagttgt
    32221 gctataataa tgtagttgat aatgtaaaaa gaaagttaat ataattaatt tttttaaata
    32281 aagagaagag gtgactataa tgcaaaagga aatacaacca aaatacaatc cagttgaagt
    32341 gcgttgtgct tgtgggaaca catttgttgc tggttcaact aaggacgaaa taaaagttga
    32401 aatatgttca gagtgccatc cattctatac aggaaaacaa aagaacatag aaaaaggtgg
    32461 aagaatcgac aaattcaaga aaagattcaa aatggactaa tttttataaa agtggagttg
    32521 gaggaagcca actcctattt tattacaaaa aaacattcat attctacttt aaattgtctt
    32581 tttgtattat aataataaat atgtaaaatt gagtattcta aatacaataa aatgaggtga
    32641 gtggatggca aaacaagctg taggtgggca agctgttatt gaaggtgtaa tgatgcagtc
    32701 aaaagacaaa agagcggtag ctgttagaaa aagtgacgga gaaatagcac taaaagaaga
    32761 tagaataaaa agttgggtaa gagataagaa tatagataag ataccttttg ttagaggttc
    32821 ttttgttatg atagatacaa tgattcaagg cataaaaagc ttaaactttt cttcagagtt
    32881 ttttatggaa gaagctgaag aggataaatt tgatttattt ataaagaaaa tttttaaaga
    32941 taaggcaaat gatataatta taatattttc attggtcata gcaatgttac tttctgctgg
    33001 attattcatt tttataccta cattggtagg aggagctttt tctaaagtaa tgcctaatga
    33061 ttttatgctt aatttaatag aaggaatcat aaggatagct atattatttg catatatagt
    33121 attgatttca cgaagtaagg atatagaaag agtctttcaa tatcatggag cagaacataa
    33181 atctatatat tgctatgaaa atgatttaga acttacagta gagaatgcta gaaaatttaa
    33241 aagattacat cctagatgtg gtacgaattt cctatttata gttatggctg tatcaataat
    33301 attgtttgca ttttttggtt ggccaaatcc aattcttaga atatttatga gaattatatg
    33361 tgtgcctata gtagcaggtt tatcttatga ggtaattaga gttcttggga aatatgataa
    33421 tggatttact aaaattattg catatccagg aatgatgttg caatatttta caacaaaaga
    33481 gcctgatgat gagcaattag aggttgcttt ggaagcattg aaagcagtag tagattagag
    33541 aaaaggatat ttgttatgac gataaaagat ataattatta aatattcaga taaactaaaa
    33601 gatataagtg atactccaag attagataca gagttattgt tacaaaaaac attgggtgtt
    33661 gatagattat atattcactt aaatctgaat aaagagctaa ctgaagaaca aaaaacaaaa
    33721 tttatgggtt ttgcagaaga aagattaaat ggaagaccaa tagcatatat agttgaaaat
    33781 agggagttta tgggattaga tttttttgtt aaggaaggcg tattgattcc aagaccagat
    33841 acagaaactt tagtagaaga aataattgaa atatgtagag aaaaaaaaga tgtaagtata
    33901 ttagatatag gaactggttc tggagctatt acaataagtt tagcaaagta cattgaaaat
    33961 tctaaaatca tgtcttttga tatatcagag acagcattag aaatagctaa aaaaaatgct
    34021 attataaatg aagttggtga aaaaataaag tatattaatt ctgatttatt tacagcaata
    34081 agtgatagta acataaaatt cgatatcata gtttcaaatc caccatatat taaaaaacaa
    34141 gatatagaaa ctctgcacaa acaagttaaa gattatgaac catataatgc tcttgaaggt
    34201 ggagaagatg gattagactt ttatagaaga ataacagaac aaggtaagaa atatttaaat
    34261 aagtgtggaa tattagcata tgaagttggt cataatcaag ctgaagatgt aataaatatt
    34321 atgaaaagta atggatataa aaaaatatat acaaagaagg atattcaagg cattgataga
    34381 gttgttatag gttacaatat atgatataat caacaattga tatgttacat ttaatcgagg
    34441 tgaaagtatg ttaaaaaaat tagaggtatt ggaagataca tataaagact taagtgaaaa
    34501 aataggtgat ccggatgtaa taaatgatca aaaagtttgg cagaaatata taaaagaaca
    34561 tgctgacttg gaacctattg ttatgaaata cagagagtat aaaagcgttt tagatagtat
    34621 aaaagaatca aaagaaattt tacaagaaga gtcagatgaa gaattaagag aattagctaa
    34681 aatggagtta gctgagatgg aagaaaaagt agcacctctg gaggaagaaa taaaaatact
    34741 attattgcct aaagacccta atgatgataa gaacgttata gttgaaatta gaggtggagc
    34801 tggaggagat gaagcagctc tatttgcggg tgatttattt agaatgtact caaggtatgc
    34861 tgaaagaaga agatggaaaa tagaattatt gagtgcaagt gatactggag ttggtggata
    34921 taaagaagta tcttttatga taaaaggtaa aggtgcttat tcaagattaa agtatgaatc
    34981 tggagttcat agagttcaaa gaataccatc aactgaatct ggtggaagaa tacacacatc
    35041 aacttctact gttgcagtat taccagaagt tgaagatgta gaggttgaaa taaatccaaa
    35101 tgatttaaga atagatgttt tccgttcttc gggtaatgga ggacaaagtg tcaatactac
    35161 tgactctgct gttagggtaa cacatatacc tacaggtgaa gtagtatctt gtcaagatgg
    35221 aaaatcacaa ttaaaaaata aagagcaagc tttaaaaata ttaaaagcta gactttacga
    35281 taaagcattg gcagaacaac acaaagatat agcagcagaa agaaaaagcc aagtcggaac
    35341 aggagacagg tcagaaagaa taagaacata taatttccct caaggtagaa taagtgacca
    35401 tagaataaat ttaactttat ataagctaga tgcattctta gatggagata tagatgaaat
    35461 gatagatgct ttaattactg ttgaccaaac agaaaaaatg actgcaatat aagtaatgtt
    35521 ttaaagaagc tgtttcttcc tagaaattat tttctatttt gaaacagcta ttttgcattt
    35581 taggtcatgt tatcatattc tattgtatat aattgttata tacaagtata ggagggttat
    35641 atgatactaa aagtaacaat aattgggctt ttggcaggtg ttatagggac aggattagga
    35701 ggagtaatat cagccatttt taaaagagag gtcgataaat atctaagttt ttttatggga
    35761 ttatctggtg gaataatgtt ggctgtagtg gtgtttgatt taatgaaaga atccatggac
    35821 aaaatgggga taatcaatac agttatattt acgtttgttg gagctctcat aactatgtac
    35881 ataaaaacaa aattagatgt gtctggaaat atggcatctg gatatcttat attcataagt
    35941 atattactac ataatttacc agaaggacta gccattggtt cttcttttat gtcaactgaa
    36001 agtttaggta ttacacttgc tatagttata ggtctacata atataccaga agggttagct
    36061 atggcattag gcttagtttg caataaaatg aaactaagta aagttatttt gtttactgta
    36121 atagctggat tacctatggg tctaggaagt tttttaggtg tttattttgg aggagtattt
    36181 acttctctta taggagtatt tcttgctaca gcaggaggta ctatgatgta tgttgtattg
    36241 gaagaaattt ttccacattc aaaaagtgtg tattgtataa ttggtttctt gttaggtaca
    36301 atgattgtaa attatatata aaattaaaat gaaagcaggc atacaaaaga tgaaaactat
    36361 tataagtaat attgatataa ataatattga ttatgaggaa ataaaaatac aagcaaaact
    36421 tcttagagaa ggtaaaacag ttatttttcc aactgagaca gtatatgggt tgggtgcaaa
    36481 tgcattagat gaaaatgcag taaagaagat ttatgaagct aaaggaagac ctagtgacaa
    36541 tccattgata gtccatatat atgaaaaaga agaagtatat gacttagcca aagatataag
    36601 tgataaagct aaacttgtta tagaaaagct ttggccagga cctataacaa ttatattaaa
    36661 taaaaaagat ataattccat acaaaactag tggagggctt gatacagttg caataaggat
    36721 gccttctaat gttattgcca gggcaataat aaaagaagct ggtataccta ttgcagctcc
    36781 ttctgcaaat atctcaggtc gcccatctcc aacaaaagca aaacatgtat atgaagagat
    36841 gaatggaaga gtggatggaa tagttttagg aggagacagt aattttggat tggagtcaac
    36901 tgtattggat ttaacagaag agactcctat gatactgaga ccaggaagta taactaaaga
    36961 agttttagag gaacttcttg gagaggtaaa gcttgaccca tcattaagta aaaaagaaga
    37021 taatcaaaaa gctaaagctc ctggaatgaa atataaacat tattctccaa atgcagatgt
    37081 atatataata tctggaaaaa gagaaaatgt agcaacaaag attaatgata tgataaaatc
    37141 taatagagac aaaggtctaa agacaggtgt aatgtgtatt agtagaaacc gagagtttta
    37201 tgatggagaa gtaatcgatt taggaaactc tcttgaagaa gttgcaagta atctgtttga
    37261 tgctcttata gaaatggaca aaaagggtgt tgatgtcata tattcagaag agttccctaa
    37321 gactggagtt ggacaagcaa ttatgaatag actactgaag tcagcaggat acaaagtgat
    37381 aaaggcttaa gagggagttg taaaatatga atatacttat tgtgtgtaca ggtaatactt
    37441 gtagaagtcc tatggctgag gctatactaa ggaaagcaat taaagaatct ggaagaagta
    37501 ttgaagaata ttctatatca tcagcaggta tatctacagc aaatggaatg ggtgcttctg
    37561 aaaattctat agaagtacta aaagaaatcg gtatagactt atcaaatcat agaagtaaag
    37621 taattactaa aaaattaatt gatgaatcag atataatact aacaatgact aaatctcata
    37681 aagaaatttt agtacaagca gttccaaaat gcaaagaaaa agtgtatact tttaaagggt
    37741 ttgcaaataa aaatgaggaa gatatatctg acccatttgg tggaaatttg gatatttata
    37801 gaagcactat gagagaaata atgtattctg tcaacgaaat agtaaaaaaa atatgaaacg
    37861 gaggataaac aaatgaagat aggattaggc tgtgaccatg gaggatataa tttaaaaaaa
    37921 gaaataataa gctatttaga aagtaaaggt atagaatgtg tagattatgg aactaataat
    37981 gctacagatt ctgttgatta tcctgtttat ggagaaatag ttgcaaattc agttataaat
    38041 aaagaagtag attatggaat attatgttgt ggtacaggga taggaatatc tttagctgct
    38101 aataaggttc ctgggataag atgtgcagtt gtatcagatg tattttctgc aaaaatgtca
    38161 aaagcacata atgatgcaaa tatgttatca ttaggcgaaa gagttttagg aaaaggtcta
    38221 gcacttgaga tagttgaagc atggattaat acggattttg aaggtgatag acatgctaga
    38281 agagtaaaca tgattaaatc aatagaagaa aaacataata aataataaaa attacagttt
    38341 tatcaggagg aattatcaaa atgagcaaag tggtagagac aaaccatcca ttaatacaac
    38401 ataaattaac tttaatgaga gataaaaata ctggttctaa agactttaga gagcttttaa
    38461 ctgaaattgc aatgttaatg gggtatgaaa taacaaaaga tataccatta aaagatgtag
    38521 aaatagaaac tcctattcaa aaaacatcat ctaaagtagt tgcaggtaaa aaattagcaa
    38581 taattccaat tttaagagca ggtcttggaa tggtagatgg attagtgagc ttaatgccag
    38641 cagcaaaagt tggacatgta ggactatata gagacccaga aacattaaaa ccagttgaat
    38701 attattgtaa gcttcctcaa gacataggag aaagagatat aatagtagtt gaccctatgc
    38761 ttgcaacagg gggttctgct gtagcagcta tagatttact taagtctaaa ggagctaaaa
    38821 gtataaaatt agctaattta gtagctgcac cagaaggtat agcagaagtg caaaaatatc
    38881 atgatgatgt tgatatatat gttgcatcag ttgacgaaag attaaatgaa catggatata
    38941 taattcctgg tcttggtgat gctggagata gattgtttgg aactaaataa aaataaataa
    39001 ctataaatat ttattaggga ctttcttata tagtgtatat aattttatgt aagaaggttc
    39061 ttttttatat attttttctc aaaataggaa ttattgaatt gataaatttc tcgaattgag
    39121 aataaaaagt ttttaccaat taaaaaagat tgcttatttt acaaggtttt atagaatagt
    39181 agtctatttt aatgtggcat gttagttgct tacgattata ggtattaagt gtattagtta
    39241 aattgagaat tgaatattaa taatattatt gggagtggta atacatgaag aattgtatag
    39301 tagtaagagg ggctggagat ttagcaacag cagttattca taaattacac aacagtggtt
    39361 ttaaagtagt tgcgttagaa atagaagagc cattagctat aagaaggcaa atttcttttt
    39421 gtgaggctgt ttatgaaaaa aaagtaactg ttgaaggtgt agaatgtata ttttgcaata
    39481 ctgaaaaaga aatagaggaa gttttatctc aacataaagt agccttgatt atagacccaa
    39541 aaggagaaaa attatctaaa ttaaatccaa tcgttttggt tgatggaata ctcgctaaga
    39601 aaaacttagg tacaactaaa gatatggcac cacttacaat agctttaggt cctggatttt
    39661 gtgcaggaga agatgtagat gttgttatag agacaatgag aggtcataat ctaggaagaa
    39721 taataactaa tggtagagca gaaaaaaata caggagtacc aggagcaata aaaggttttt
    39781 ctaaagagag agttatgcat tcacagtatg atggcataat ggaaaatgta tgtaagatag
    39841 gtgatatagt tgaaaaggga gacctacttt gctatattaa taattatgaa aaaaaacatg
    39901 aagttagagc aacaatatct ggagtcctta gaggtttatt aaaggatgga gcaaaggtaa
    39961 acaaaaattt aaaaatacta gacatagacc ctagaaaaga agaagttaag aatgctttta
    40021 caatatcaga taaagctaga tgtatagctg gaggggtttt agaagccata ctaaacaatg
    40081 ggatttatcc ccaaaattaa tttatattag ataaagctat taatatttta ttaagatgat
    40141 ataataaatt taatagatta aagagagcat atgacgggta tttaaaattt aaatggtaaa
    40201 aatgctcttt ttttattgga cattcaatta acatgtgtat ataatataaa aataagtatt
    40261 tatttaggtt gaaatctaat aaatatataa taccagtaca taagcaaaga ggagaaaagt
    40321 aaattatgag accatcatgg gatgaatact tcatggaaat tgcagaagta gtgaaaaagc
    40381 gttcaacttg tattagaaga caagtaggag ctgtaattgt aagagacaaa caaatattaa
    40441 caactggata taatggttct ccgagaaatc ttgaacactg tgaaaatatt ggatgtaaga
    40501 ggcaggaatt aaatattccc tcaggagaaa ggcatgaatt atgcagagca ttacatgctg
    40561 aacaaaatgc gataatacaa gctgctcata atggtattag tgtagatggg gcaactttgt
    40621 atgttactac aagaccttgt gttttgtgtg caaaaatgtg cataaatgca ggtatattga
    40681 aaattgttta tgaaggtgat tatccagatg atatgtctac agaattatta aaagaagcag
    40741 gaatagaact cataaaattt tagtggataa aatttataag acaataaatg tcaaattatg
    40801 acactgaaat acaatggtat caaatgatta aaagaaagta tttgaaaaat catcagatta
    40861 gtagtaagat atattttata attaaataaa tttatgagat agattaatta catgttattt
    40921 attttataat aatgagggat actatttatg agtaaaagaa atacacatac agaaattgca
    40981 aagttgttta gtttaataag tcaaatagga ttgatgattg taatatcaat attaggatgt
    41041 actttcttag gaaaattttt agatagtaaa ttgaatacta ctccagttct gacaataata
    41101 tttttattgt tgggtgttgg aggtgctttt agtggtgttt ataaaactct aatagtatat
    41161 acacgaagga agtgatatat tggatataaa attacttaac caggtaaggt cagtaacaaa
    41221 aggtatagta atatttgatg tagttttaat tgtgctaatg ttgataacat caacattaag
    41281 taaaccagta ttattaggac ttatatttgg ctctataata gctatgttaa attttagatt
    41341 attggcgata tcactggaaa aatcagtgac atttcctgct gggaaggcac aagcttatgc
    41401 atcagcacaa tatgctgtta gattatttat aatggcagtt gttctaattg catcagctac
    41461 agtaccacat ttaaatataa tcggaacagc attgggatta ttgagcacaa agtttgtcat
    41521 attatcaaaa aactttataa ataaattaaa gaggaaggag gcataagtat gaatcaagcg
    41581 cattattatc tcataggaaa ctttaagatt agtgaaacaa tactagtaag ttggggaata
    41641 atggcctttt tagcgatagc atcttatttt ttgactagaa acttaaaaaa agttccaact
    41701 agtaaagttc aaatattctt agaatttgca gtaggtggtc ttgctaaaat ggttacagat
    41761 gttatgggta aaaatacagt aaaaagaatg ccatatatga taccatacat aggaagcttg
    41821 tttttatttt tcgcatgttc aaatttaagc ccattacttg gattaaggtc accaacaacc
    41881 gacttagaca caactgctgc atgggcaatg attacatttt ttatgattta ttttgcagga
    41941 gttaaattta aggggttatc atactttaaa gaattggctc aaccacttgc cataatgtta
    42001 ccaatcaata ttataggtga gttagctaga ccaatatcat taagtttccg tccatttggg
    42061 aatattttag gtggaacaat aataatgggt ctgttttatc aacttttagg ttttatatct
    42121 agtttaatac caggattatc aataccaatt ggagaaatat taataccagt cccattacac
    42181 ttgtactttg acttatttgc aggattgtta caagcatata tatttataat gttaacaatg
    42241 gtatttatat caagtgcagg agagttagat taagaaatca acttatatta agataaaaat
    42301 aaaataattt aaaaaattaa agttttaagg aggaaataat tatggaaaca gcaatagtag
    42361 cggcagcatc agcaatagga gcagggatag cagttgcaac aggtataggt gcaggtatag
    42421 gtcaaggtat agcagcagct aaagctgcag aagcagtagg aaaccagcca gaagctaaag
    42481 gtgatataac atcaacttta ttactaggag ttgctatagc agagtcttca gcaatatatg
    42541 gtttggttat atcaataata cttctattcg taaatccatt ctttaaatac ttaggaatgt
    42601 agatttagtc cttaaatatt ttgagatagg tttataggta aatcatctca aacttataat
    42661 catgagatga actttatcat ctcgtgatta taagtagtta tgatttatgg aaaggaggat
    42721 aattatggaa aaagcactag taggtataac gtgggaattt gtgttccaaa ttgttaatac
    42781 ttttataata tttttacttc tgagaaagct tttattcaag ccagtattaa atataattga
    42841 atcaagagaa aatgatatta aaagtgattt ggcagaaggc gaaaaagcaa aaaatgaagg
    42901 tttagctctg aaaaaagaat atgagtcaaa aatcaatttt gccaaagatg aaggtcaaga
    42961 aatcataaaa caagcaacta taagagcaga gcagaaatca gatgatatag taaatactgc
    43021 aaagaaagat gctcttgata taaaagaaaa agcaaacaaa gatatagaac aagaaagaca
    43081 aaaggttata aatgaaatca aaaatgatat atctaacata gcactacttg cagcatctaa
    43141 agttattgaa aaagatttag ataaatctaa acatgaagaa ttgatagaaa actttataaa
    43201 agaggtaggg gaagctaaat gataaatgta atagcaaata gatatgccga agccctattt
    43261 caattaggag aagaagaaaa ttcaactgat gtcttattca aagaactaga aaaagtagta
    43321 gatatgatga ctaaagttag taaagatttc tacaaagttt taaaatcacc attagtttca
    43381 aaatcagaaa agaaaaactt ggtagaaatt atttttagta aggaagtaag ttctaacata
    43441 aaaaactttt taaaagtttt agtagataaa gatagaatat catatttaga agatatcgaa
    43501 ttagcttata aagaattatt aaataagaaa aacaatgtaa ttgatggagt agcaataagt
    43561 gctattccaa tgagtgaaac agatattaaa gaacttgaag tcaaactttc aaataaatac
    43621 aacaaaaatg ttactataga aaatgttgtt gacaagacta tactaggggg agttctagtt
    43681 agaattggaa atgaacaaat agatggcact gtgaaaactc gtcttgataa gatgaaagaa
    43741 aagctatctg aagtaatatc ttaagaatgg cggtgaaccc atgaacttaa aacctgaaga
    43801 aataagttct ataattaaac agcaaataaa aaattatgag aataaagttg agttgacaga
    43861 tacaggtagt gttttaacag ttggggatgg tatagcgagt gtatatggat tagaaaaagc
    43921 tatgtctggt gagttgctag agttcccagg tgaaatatat ggtatggcac ttaacttgga
    43981 agaagaagta gttggagctg ttatccttgg agatgacagt gaaataaaag aaggcgatat
    44041 agttaagaga actggaagaa tagttgaagt tccagttgga gaagctttaa taggtagagt
    44101 tgtaaactca ctaggtcagc caatagatgg aaaaggacct atagcgtaca caaaaactag
    44161 accagttgaa tctgaggcac ctggtataat agatagaagg tctgtttatg aaccattaca
    44221 aacaggaatc aaatcaatag attcaatgat accaattggt agaggtcaaa gagagttaat
    44281 aataggtgac agacaaactg gtaaaacatc tatagttatt gacactatac taaatcaaaa
    44341 aggaaaagat gttatctgta tatatgtagc aattggacaa aaacgttcta ctatagctca
    44401 attagttagc tctttagaaa aaggtggagc tttggattat acaatagtag tttcagcaac
    44461 agcatctgaa tcagcaccac ttcaatatat agcaccatat gctggagcag ctatgggtga
    44521 agaatttatg tacaatggga aacatgtact tatagtgtat gatgatttaa gtaaacaagc
    44581 tgttgcatat agagaaatgt cattgttact tcgaagacca ccaggacgtg aggcatatcc
    44641 tggagatgta ttctatttac attcaagatt acttgaaaga gcagctaagt tatctgatga
    44701 attgggtgga ggttcaatga ctgccttgcc tataatagaa actcaagctg gtgacgtttc
    44761 tgcatatata ccaacaaatg ttatatctat aacagatggt caaatatact tacaaccaga
    44821 gttattctat tcaggtgtaa gaccagcagt tgaccctggt atatcagtat caagggttgg
    44881 tggttctgcg caaattaaag caatgaaaaa agttgcagga acattaaaac ttgcatattc
    44941 acaatataga gaacttgcag cattctcaca gtttggttct gacttagatg aagatactaa
    45001 aaagagactt gctcaaggtg aaagaatcgt tgagatatta aaacaaggtg agcatcaacc
    45061 aataaaagtt gaaaatcaag ttatgataat atatgctgtt ataaataatc atttagagga
    45121 tattccgata gataatatag ctagatttga atcagaattg tatgcatttg tagataataa
    45181 ttatccggaa atatcaagaa aaatattagg tggagaagac ttcactcatg atttaacaga
    45241 tgcaataaat gaatttaaag aaaagtttgt tgttgaagta taatcctttt taaaaaagga
    45301 ggtaaattaa ttggcaggag ctggaatcaa agcaatcaaa acacgtatgg gaactgttga
    45361 aagtacaaag caaataacta aagcaatgga attagttgca tcttctaagc ttagaaaagc
    45421 aaaagaaaag gctgaagatt caagagaata ttttaatact ttatacaata cagtaaaaga
    45481 tattgcccaa acaacacgtg gaattagaaa tgaattttta aaagaaagag aagtaaaaaa
    45541 taaatgctat gtagtagtag ctggagacag aggacttgca ggtggataca attctaatgt
    45601 attaaaggaa tctgtctctc atatgaaagg taaaaaggaa acagtgatta ctgttggaaa
    45661 gaaagcacat gaattttttt ctaaaagaaa ctacaatata gcaaaagata taaatagtgt
    45721 tgaagcttgt ggatatgaag atgcgacaga aatttcacaa actattattg acctgtataa
    45781 aaaaggtgaa gtagatgaag tttatattgc ttatacaaaa tttaaatctg ctttatcaca
    45841 agaagttgaa attttgaaat tgcttccatt agtctttgaa aaagatacag acgaacaagg
    45901 aatggcagta aaagaagaaa aacaaaatag atcaaaagta caatatatac catcagcaga
    45961 agcagtttta agttatattg tacctcagtt tatatcaggt acagtatatg gtggaatttt
    46021 ggaatctttt gcatcagagc aaggtgctag aagaactgca atggaatcag ccacagacaa
    46081 tgcgaatgaa atgctatcta atttagagtt aagttataac agagcaagac agtcagctgt
    46141 aactcaagaa ataacagaga tagttggtgg agttgaagct ctaaaatagg gaggttagga
    46201 aatggcaaat gtaggtaaag tagtacagat tgtaggagct gtactagatg ttaagtttga
    46261 tagtgaacaa agcctaccta acttattaaa tgcgttagta ataaagttag gagataaaga
    46321 aatagttgca gaagttgctc aacatatagg tgatgatact gtaagatgta tagctatgag
    46381 tgcaacagac ggacttgtaa gaggtatgga agttgtagat acaggagggc caataagtgt
    46441 tcctgttgga gatgaaactt taggtagaat attcaatgtt cttggaaaac cagttgatgg
    46501 gaaaccagct ccaaaatctg cacctaaatt acctatacat agaccagcac cggcgtatga
    46561 tgaactagaa actacagcag agatactaga aactggtatt aaggtagttg acttactagc
    46621 tccatatcta aaaggtggaa aaataggtct atttggagga gcaggagtag gaaagacagt
    46681 tctaatacaa gagcttataa acaatattgc taagcaacat ggtggtattt ctgtattttc
    46741 aggagtagga gaaagaacaa gagaaggtaa cgacctttat ggcgaaatga gtgagtctgg
    46801 agttataaat aaaacagctc tagtatttgg tcaaatgaat gaaccacctg gagcgagaat
    46861 gagagttgct ttaactggac ttacaatggc agaacatttt agagatgagc aaggacaaga
    46921 cgttttactt ttcgttgata atatattccg tttcacacaa gctggttcag aagtttcagc
    46981 acttctagga cgtatgccat cagctgttgg ttatcagcca acattagcta ctgaaatggg
    47041 tgcacttcaa gagagaataa catcaactaa gaaaggttca ataacatctg ttcaagcagt
    47101 atatgtacca gcagatgact taactgaccc agcaccagct acaacattct ctcacttaga
    47161 tgcaaaaaca gttttatcaa gacaaatatc atcactgggt atatatccag cagttgaccc
    47221 tcttgaatct acatcaagga ttcttgaccc tagtatagta ggaaaagaac attatgaagt
    47281 agctagaggt gtacaatcaa tacttcaaag atataaagaa cttcaagata taattgctat
    47341 acttggtatg gatgaattat ctgatgaaga taagttaata gttgcacgtg ctagaaagat
    47401 acaaagattc ttatctcaat cattcacagt tgctgagcaa tttacaggaa atccaggtca
    47461 atatgtacct gttaaagaga ctgtaagagg atttaaagaa attcttgaag ggaaacatga
    47521 tgacttacca gagtcagcat tcttatttgt aggaacaata gaagacgctg ttagaaaagc
    47581 gaagggaagt atgtagtcat ggccagtgaa ttttcattga aagttgtaac tccaagtaat
    47641 actttttatg atggagatgt tgaaatgata gtagttagaa ctacaaaagg agacaaaggg
    47701 gtgttgaaag gtcatatacc atttgtttca ggtatacctg taggaacttt aaagataaaa
    47761 aaagagggaa gttttaaaga ggcgaaaata gcagaaggat tcattaatgt aactcaagat
    47821 aaaactgtca tattgacaga atctgctgag tggatttaat aaattaattt agagtataaa
    47881 aagtcatttt gaccaaaaaa acttttgggt aaaatggctt ttttgcgttt tggtgttaat
    47941 aaaataacga taaaattgta agtttttagt gacaataaat tcacaaaatg tttaaattta
    48001 ctgccaaatt atgttgtaat taataagata aagaagattt agatagcttt tatgtaagtg
    48061 aaaaagtctt catataagaa atacatataa aatttttata aaatttaaaa aatttgctta
    48121 gttgtgtagt ataataaata atataataga tttgtatata atattcatta caaataattt
    48181 taaaagttac agttttatta taatgattat taaattaaga taagtaacat aaactaaata
    48241 ataatgtgta ttatcgatag taaatttata aattcattat taattattga aagaagtatt
    48301 ataaattttc tggaggaaca atgaatatat ttgatattgg cgtagatata attgaaattg
    48361 atagaataag aaaggctgta gacaaaaata ataggttttt ggagaagata tttacagata
    48421 gagaaataga gtattttaat tctaagaatt ttaaagcaga aagtatagca ggtaatttcg
    48481 cagccaagga agctataagt aaatctatag gtacaggaat tagagtgttt aattttaaag
    48541 atatagaagt tctcagagat gaaatgggaa aaccaatagt aaaaacttat aataatctag
    48601 caaaaatgtg catagattat aatgttttag aaataaaagt ttcaatatca cattcaaaag
    48661 attatgctat agcaaatgcg ataaccataa ttaaggacta aggagtgatt acattatgat
    48721 ggataaaaca gcaaaagaaa taatgacaac agatgtaata gtagcaaaac aagatgatag
    48781 tatagcagat gtagctaata tgctaattgc agaaaaaata ggaggtctgc cagttgtaga
    48841 ttcagagaat agagttgttg gaataatttc tgaaacagat attcttaaaa aagaaaaata
    48901 tatagaagca cctctctata taaatctatt acaaggattg attttcttag atgacttaaa
    48961 aaaagttgaa aaagatataa aacaagtagc tgcttataag gttggagaac ttatgtctaa
    49021 agatataata aaggttcatg aagatgataa gtttgatgat gtagctaatg taatgattaa
    49081 aaaatcaata aacagagttc ctgtagtaga tgatgataac aaacttaaag gtataatttg
    49141 tagatatgat ataataaaag ctttatacaa tgaataaact gaagtaggag gtgtgatgtg
    49201 agaaaaaagt ggaccatagt atgtattatg ttcttggcac tggttataat tgtaatagga
    49261 tgccaaaaac gacagtccac aaaagaagaa gtatacaaag attttcaaaa gcagatttct
    49321 gatatgaatt attacagttg taaagctgaa gtagaagtag ttggaaataa aagtccacac
    49381 aattatgttt taattcatac ttataaaaaa accgataact ataaattaga agtaatttcc
    49441 ccaaaacatt taaaagggaa aagtatagaa tatcaaggtg ataaaatatt agttaaaaat
    49501 ccaaagatta gtgatgtagt ggagttacct aatacaggaa aaaataatca atatcttttt
    49561 gtaggagact ttataaaaaa ttatcttcaa aatgaggaga tgaaagtaaa gttatccaaa
    49621 ggacatttgg ttttagagac ttttattcct ggggataata aatattttaa caagcaagta
    49681 ttatatgtaa atgctgacac aaaaaatcct gaaaaaatgg aagtgttaga taaagaggga
    49741 gtgccaagat ttacagtaaa atacaaagat tttgaataca gaaactaagg gggactaaag
    49801 acatgcaaaa aataacagtg cctacatggg cagagataaa tctagataac ttaagattta
    49861 acttaaataa tattaaaaat ttattagaag aagatattaa gatttgtgga gtaataaaag
    49921 ctgatgcata tggacatggt gcagtagaag ttgcaaaatt gctagaaaaa gaaaaagtag
    49981 attacttagc agtagcaaga actgctgaag gaattgaact tagacaaaat ggcataacac
    50041 ttcctatttt gaacttggga tatactccag acgaagcttt tgaagattct ataaaaaata
    50101 aaataactat gacagtttat tctttagaaa cagcacaaaa gataaatgaa attgcaaaat
    50161 ctttaggaga aaaagcctgt gttcatgtta aaatagactc agggatgact agaataggtt
    50221 tccaacctaa tgaggagtca gtacaggaaa taatagaatt aaataaatta gaatatatag
    50281 atttagaagg tatgtttact cattttgcta cagctgatga agtaagtaaa gagtacactt
    50341 ataaacaagc taataattat aaatttatgt ctgataaatt agatgaggct ggtgtaaaaa
    50401 tagctataaa acatgtatca aacagtgcag ctattatgga ttgccctgat ttaagattaa
    50461 atatggtaag agcaggaata atattatatg gtcattatcc atctgatgat gtatttaaag
    50521 atagattaga attaagacca gccatgaaat taaaatcaaa aatcggacat ataaaacaag
    50581 ttgaaccagg tgtaggaata agttatggac taaaatacac aactacaggc aaagaaacaa
    50641 tagctacagt tccaatagga tacgcagatg gatttactag aatccaaaaa aatccaaagg
    50701 ttcttattaa gggagaagtg tttgatgtag ttggtagaat atgtatggat caaataatgg
    50761 ttagaattga caaagatata gacataaaag ttggagatga ggttatacta tttggagaag
    50821 gcgaagttac agctgagcgt atagctaaag acttaggaac tataaactat gaagtgttat
    50881 gtatgatatc aagaagagtt gaccgtgttt atatggaaaa taatgagctt gtacaaataa
    50941 acagttattt gctaaaataa aaccatgata ttataatctg ggaggcgatt ttgtgtacga
    51001 taagagtaaa gaaaaaatag aagttacttt acctgactct ctgatttctg aagttgatag
    51061 tatagttcag atggagaaca gtaacagaga agattttgca aaagctgctt ttcagttcta
    51121 tataactcag aaaaagaaaa ttcatattaa agaatctatg aaaacgggtt atagagaaat
    51181 gggtcaaatt aatctgtcac tagctgaatt aggtatgaca gttgaagaag tatcttctga
    51241 tgataaaaat gaagggaaga ttgcccaagg tgagtactaa ttgtgaaata aagcgtggtg
    51301 atttatatta cgctgattta agtccagttg ttggttcaga gcagggaggc gttagacctg
    51361 ttctgataat acaaaatgat gtaggtaata aatatagtcc tacagttata gtatcagcta
    51421 taacatctca gattaataaa gccaaattac ctactcatgt agagataagt tccaatgaat
    51481 atgggcttaa taaagactca gtaattcttt tagagcaaat tagaactatc gataaaaaga
    51541 gactgaggga aaaaattggt tgtctagatg aaaatatgat gataaaagta gatgatggac
    51601 ttcaaataag tttaggattg tttacttttt aaagagggat ttgtctttta ttagacaatc
    51661 ccttttttct tttaaatatc tcttaaggtt taattttaat gtactatgtt aataaaatta
    51721 aaaataacgc aaaaaaatag cataaaagtg atataataat atggaattga tataaaatta
    51781 cgaactcaag ggaggtaatt gcatgagaga ccataaagga ttaaatgaga ttgcgcgtat
    51841 cataagaaga gatatagttt caatgataca tagagccaaa tctggacatc caggagggtc
    51901 attatcagta gttgaaatac ttactgcgtt atactttgat gaaatgaatg tggattcttc
    51961 aaatcctaaa atggaggata gagatagatt tgtattatca aaaggacatg ctgcaccagc
    52021 attatatgct acattggctg aaaaaggtta ttttgataag gaagaactga atggattaag
    52081 gaagatagga aggatgttac aaggtcatcc agacatgaaa ggcacgccag gtgtggaaat
    52141 atctactggt tctttaggtc aaggtttttc agttgcatgt ggtatggcta tggcatcaaa
    52201 gttggataat gctccgtgga atgtgtatac tttgcttggt gatggtgagg ttcaagaagg
    52261 aattgtttgg gaagctgcta tgagtgctgc acattataaa cttgataatt taattgcttt
    52321 tttagataat aatggacttc aaatagatgg agatatagaa agcgtaatga gtctaggttc
    52381 aatagttgat aaatttaaag cttttggttg gaatgtaatt gagatagatg gacatgattt
    52441 tgaccaaata tttgcggctc ttgatatagc taaatcaaca gttaaaaaac ctacaatgat
    52501 agttgcaaaa actataaaag gcaaaggaat atcttttatg gaaaatcaag caggatggca
    52561 tgggacagct ccaagtgatg aggagctaga aaaagccttg cttgaattag gaggtgctga
    52621 caatgagtaa aatggcgacg agagaagcat atggtaaagc attagtaaaa ttaggtcaaa
    52681 taaatgacaa tgttgtagta ttagatgcag atttatcaaa gtctacaaaa acacatgatt
    52741 tttacaagtc atttccagat agatttttta atatgggtat agctgaacaa aatttaatag
    52801 gcgctgcttg tggattatca actgctggta aaataccttt tgcaagtact tttgctatgt
    52861 ttgctacggg aagggctttt gagattataa gaaattcagt ttgttatcca aagctaaatg
    52921 taaaaatatg tgctactcat gcaggtctta ctgttggaga ggatggagca tctcatgaaa
    52981 gtgtggaaga tattgctata atgagagcta taccaaatat gacagttctt gttccagctg
    53041 atggagttga aacagaaaaa attatttttg aaatagctaa gtataatgga cctgtttatg
    53101 ttagattagg gagaagttca gtaccagttt tatttgatga agattataaa tttgaaatag
    53161 gaaaaggtac agtcttaaga gaaggaaagg atgtatcaat tatagcttgt ggaattatgg
    53221 taaatgaagc tctattagca caagaaaagt tgcaagaaga aggtataagt gctagagtta
    53281 taaacatgtc ctcgataaaa cctatagaca aagatttaat attagaatca gcaaaagaaa
    53341 cgaatgctat tgtaactgta gaagaacata gcataattgg gggattaggt tctgctgtaa
    53401 gtgaggtagt aggagagtct tgtccaacca ttgtaaagaa agttggaatt aaagatacat
    53461 ttggagaatc tggtactcca aatgaattgt taaaaaaata tgaattaact tgtgatgaca
    53521 taataaagac tgttaaagaa gctattattg caaaaagaat gtaagagata taaattattc
    53581 atgtttgtta ataatataat atatttaaaa gagaggtata aagtgttata ttaatttata
    53641 tctctctttt ttttatatga tatcaatata tcaaaaatgt aataaaatat tcaaattttt
    53701 acaaaaatag agaaaatatt caaatatata ttgactattc agaaaaatat gactaatata
    53761 taatttatag agaaactaat tttatatata atgactactt agataaaaga atctaaaaaa
    53821 attatgagag gaggagtagg gtataaaatc agattataag ttttaagtta ttatactaaa
    53881 aagctataaa acagttatca tgtattttat gttaaaggaa attgtaggta gtttatcgaa
    53941 ttgtttaatc agaagaaaaa atttcacatg gggaggtaac tgagatatgt cagaaaaagt
    54001 aaatgttaaa acagtcatca gtttcgcagg tgcgtatgta gcaacagtaa tcggttctgg
    54061 atttgcaaca ggacaggaaa taatgcaatt cttctctttt tatggttttg caggtattat
    54121 tggtgggata atttcaatgg tattattctc atggatgggt gcttcagtta tgtcaaaagg
    54181 taaagagctt caacttaaag aacctatcaa aatttatcgt gtttattgtg gtaagtattt
    54241 aggaatattc tttgaatggt ttggaccttt attcttattt ggtgttttcg tagtaatgat
    54301 ttcaggagca ggagcaactt taactgaata ctatggatta aatccatttg taggtagagt
    54361 aggtatggca gtagtagcat tattaactgt ttcactaggt cttgataaat tatcaaagat
    54421 actaggtgga atagggccta taataataat ctttacactt ttagttggtg gtataagttt
    54481 agctaaaaac ataggtaata taggagaagc tgcacaagtt cttagcactg taaagatagc
    54541 taaaccagtt ccaaatgcct atctatctgg tgtaatatat actacttata acacaattgt
    54601 tgttatggca ttcttaactg gattaggagc aagtgctgct aacaaaaaag aagctatttg
    54661 tggtggtata ttaggtggag tagcacttat ggctgcagct ataatgatgc acttagctat
    54721 tctttctgat ataggaaact tatattcaaa agcaatacca tcattattct tagcagataa
    54781 gatttcacca gtaattggag ttttattctc aataattctt atattaggta tatatacaac
    54841 agctgtacct ttattatggt cagtaactaa tagatttgta gatgataaac atcctaagtt
    54901 taaattaata actttaatag cagctatatt aggtcttata ggtggcttcc taccatttga
    54961 taagctggtt ggtattttat atccatatac tggatatatg ggcgtcataa tactggtttg
    55021 tgttctttat agacagatta caaaaacatc tggatatcaa gaaggaatca gtgacaaata
    55081 agtatttttt aatattgtaa actcaagagc tatatgaaaa caatgaaaat gttttcatat
    55141 agctcttttt attttttagc tcgtttatta tgaaatagaa aaaacatgtt ttttagttac
    55201 attacatata tttataatta tgtataaata tatactaatc aataatttag agtctaagat
    55261 aagcttataa atcatatatt tagaatatat gggaggagtg ataagattga atgtaaagtt
    55321 taatattaaa ggtataattt atggagttat agctcttgta ttcgttatag ggggatttat
    55381 attcatacca agtatgtttg tagagaaatc taaacccata gattatacag tattacaaag
    55441 aaattcaata ccagaaaaga tattagatgt gatggataag tatacaaatg aagaaagagc
    55501 attagcggct aaaatcgata acaaaatata cattatagtg actagaggaa ataataaata
    55561 tggtatagaa atggacaaaa tagaaagtgt ggttgaggaa ggaaaggatg ttttgagagt
    55621 caagataaag tataaagata aggaagattc ttttccatac atagttgttg aaacaaatat
    55681 gagtgaactt ccagatagaa tagaattaaa tgcgacgaaa taagaaggta ttttataatt
    55741 aaataactgc atattgtaca ataaagtcat ggtttttatt aatgtaagta aattgtatat
    55801 ggttcttatt gttgtaaaag ccatgactta aaatgcttat aaagtaagat attaacagta
    55861 gtatttatta ctacattaaa tggggtctgg aaatacttaa tattctaatt atttggagtg
    55921 ataattctag tttcagtaac tatgtagtct aattgtgcat catgagtttc ttttggaata
    55981 gaatcgaata tctggaagtc aaatgctatg ccgatagtta tagcatcttt tcttaagttt
    56041 tctaaaaatc tatcataaaa tcctccacca tatcctaatc tatagcaatg tatatcatat
    56101 gctacagctg gtacaattac aacatctaag gccttaatat caattgtagg tgatttttca
    56161 ttaggttctc ttattccata tgctccaatt ctcaatccct cttttaaatc tgttatttga
    56221 gtagggatta atgtatgatt ttcttttatt gtgatgggac ttgatactat tttatttaga
    56281 gacaaaagtt ttttgattaa attatctgtt tgaacttcat tgttaaaatt taggtaaagc
    56341 attatattag ttgcatttct tatatcttcc atttgaagta atttttcagt tattaaattt
    56401 gaattactaa gtacaaagtt tgcactctta ttttttcttg attcaataac tttttttcta
    56461 aattcttttt tcataaaata attcctccct taatatgtta taatgtgtac agcaattgta
    56521 tttaatataa attttagtat aatttattgt aataaagcaa tatatttggg taaatgtaat
    56581 aaaaataaca gctagtcata taataataat agtttatgta aaaggagatg tatcttgtga
    56641 tttcaaagaa gaaagctatt tttttagggg tgattttagt aattataaca gctatggtta
    56701 catcagcatt tcagttgaca ttggggaata aagttgtaat atcgaaagaa ctctatgaag
    56761 actataaaaa atatgataag ttgttaggac tagaatctat tataaaacaa gatttttata
    56821 aaaaagtatc agatactgat ttggtaaatg gagcagcaaa aggtctattt ctaggaacaa
    56881 atgataagta ttctggttat tatactaaag atgaaatgga gaatctaatt aatgatagtg
    56941 aaggttctta tgttggtgta ggtatgtata taggggcatc aaaagatggt ggcttagtag
    57001 tagttcccat gaaagattct ccagcagaga aagcaggtgt aaaatcaggg gataaattag
    57061 taaaagtcaa tggaaaatct gtctcatata aaaattcaga tgaagctgtt cgtatgatga
    57121 agggtaaaaa aggtaaaaca gttgaactga ctatattaag agaagataaa caattgaact
    57181 ttaaagtaaa aacagaccag ataattgaga agagtataga aagtaaagta attgataatg
    57241 atttaggata tatagaaatt acccaattta tatcaagtac atatactgac tttgataaag
    57301 ctttaaaaga attaaaagca aaaaatatca agggattagt aatagacctt agaaataatc
    57361 ctggtggaat gctagatata tgtaaagaag ttgctgatga gttaattggt gaaggaacca
    57421 tagtatatac aaaagataat aagggaaata ctgagtactt gaaatctgat aaagaaaaat
    57481 tgggacttcc aatagtagtg ttaactaatg gagaaagtgc ttctgctgct gaaatattaa
    57541 cagctgcaat cgttgataat aaagaaggta tttctgtagg gactacaact tttggtaaag
    57601 ggttggtaca atcagttgta agattgaagg atggaacagg atacaagtta actactgctc
    57661 aatattttac acctaatgga gattatatca atgaaaaagg tataaagcct actattgaag
    57721 aaaaagatga aaataaacag ttagatgtag caactaagtg gcttagagaa cagattgata
    57781 aataggattt aatttttata aacttatact ataaaataaa gaacattata agagagatgt
    57841 ctcaaaatga actttttagt tcatgaggga gtctg
//
LOCUS       DAGSWI010000005        57429 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.005, whole genome shotgun sequence.
ACCESSION   DAGSWI010000005 DAGSWI010000000
VERSION     DAGSWI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.005"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            54..1292
                     /locus_tag="MJF80_000254"
     CDS             54..1292
                     /locus_tag="MJF80_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBZ0278940.1"
                     /translation="MTLKELAGDRITAAQISHIERDKSHTSYELLEYLGDKLDVSVEY
                     LLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVLDICKRYDLMENYGRCNFL
                     MANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLNIGKIYMQEDFYKGAISHF
                     NFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLKYIEKINEIDNRNDRKEDV
                     DILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLANVYLTICDVYRKIGETDR
                     VLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLARKYCKIALASSIKNKNKF
                     NEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNRILANLYIDLGQLYSNISK
                     EKELEYYQKGVFMYKNLEIM"
     gene            1397..1999
                     /gene="yedF"
                     /locus_tag="MJF80_000255"
     CDS             1397..1999
                     /gene="yedF"
                     /locus_tag="MJF80_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBZ0278941.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            2072..2335
                     /locus_tag="MJF80_000256"
     CDS             2072..2335
                     /locus_tag="MJF80_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBZ0278942.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            2350..2535
                     /locus_tag="MJF80_000257"
     CDS             2350..2535
                     /locus_tag="MJF80_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBZ0278943.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            2638..3834
                     /locus_tag="MJF80_000258"
     CDS             2638..3834
                     /locus_tag="MJF80_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBZ0278944.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            4048..4326
                     /locus_tag="MJF80_000259"
     CDS             4048..4326
                     /locus_tag="MJF80_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBZ0278945.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            4363..4797
                     /gene="ssb"
                     /locus_tag="MJF80_000260"
     CDS             4363..4797
                     /gene="ssb"
                     /locus_tag="MJF80_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBZ0278946.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            4816..5043
                     /locus_tag="MJF80_000261"
     CDS             4816..5043
                     /locus_tag="MJF80_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBZ0278947.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            5186..5506
                     /locus_tag="MJF80_000262"
     CDS             5186..5506
                     /locus_tag="MJF80_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBZ0278948.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            5514..6470
                     /locus_tag="MJF80_000263"
     CDS             5514..6470
                     /locus_tag="MJF80_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBZ0278949.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            6481..8475
                     /locus_tag="MJF80_000264"
     CDS             6481..8475
                     /locus_tag="MJF80_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBZ0278950.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            8476..8925
                     /locus_tag="MJF80_000265"
     CDS             8476..8925
                     /locus_tag="MJF80_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBZ0278951.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            8944..10272
                     /gene="dnaB"
                     /locus_tag="MJF80_000266"
     CDS             8944..10272
                     /gene="dnaB"
                     /locus_tag="MJF80_000266"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBZ0278952.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            10477..10785
                     /locus_tag="MJF80_000267"
     CDS             10477..10785
                     /locus_tag="MJF80_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278953.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            10867..12156
                     /locus_tag="MJF80_000268"
     CDS             10867..12156
                     /locus_tag="MJF80_000268"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBZ0278954.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            12482..12557
                     /locus_tag="MJF80_000269"
     tRNA            12482..12557
                     /locus_tag="MJF80_000269"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12515..12517,aa:Lys,seq:ttt)
     gene            12564..12638
                     /locus_tag="MJF80_000270"
     tRNA            12564..12638
                     /locus_tag="MJF80_000270"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12597..12599,aa:Glu,seq:ttc)
     gene            12643..12718
                     /locus_tag="MJF80_000271"
     tRNA            12643..12718
                     /locus_tag="MJF80_000271"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12676..12678,aa:Val,seq:tac)
     gene            12752..12826
                     /locus_tag="MJF80_000272"
     tRNA            12752..12826
                     /locus_tag="MJF80_000272"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12784..12786,aa:Thr,seq:tgt)
     gene            complement(13209..14201)
                     /locus_tag="MJF80_000273"
     CDS             complement(13209..14201)
                     /locus_tag="MJF80_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0278955.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(14210..15268)
                     /locus_tag="MJF80_000274"
     CDS             complement(14210..15268)
                     /locus_tag="MJF80_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBZ0278956.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            15393..16856
                     /locus_tag="MJF80_000275"
     CDS             15393..16856
                     /locus_tag="MJF80_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBZ0278957.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            16958..17755
                     /locus_tag="MJF80_000276"
     CDS             16958..17755
                     /locus_tag="MJF80_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBZ0278958.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            17768..19870
                     /locus_tag="MJF80_000277"
     CDS             17768..19870
                     /locus_tag="MJF80_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBZ0278959.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            19938..20417
                     /gene="rlmH"
                     /locus_tag="MJF80_000278"
     CDS             19938..20417
                     /gene="rlmH"
                     /locus_tag="MJF80_000278"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBZ0278960.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            20566..20877
                     /locus_tag="MJF80_000279"
     CDS             20566..20877
                     /locus_tag="MJF80_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0278961.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            20925..21242
                     /locus_tag="MJF80_000280"
     CDS             20925..21242
                     /locus_tag="MJF80_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBZ0278962.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            21455..22156
                     /locus_tag="MJF80_000281"
     CDS             21455..22156
                     /locus_tag="MJF80_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0278963.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            22380..23681
                     /locus_tag="MJF80_000282"
     CDS             22380..23681
                     /locus_tag="MJF80_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBZ0278964.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            23818..24912
                     /locus_tag="MJF80_000283"
     CDS             23818..24912
                     /locus_tag="MJF80_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBZ0278965.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKLILEGE"
     gene            24914..26047
                     /locus_tag="MJF80_000284"
     CDS             24914..26047
                     /locus_tag="MJF80_000284"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBZ0278966.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELRRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            26065..27174
                     /locus_tag="MJF80_000285"
     CDS             26065..27174
                     /locus_tag="MJF80_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBZ0278967.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            27239..27979
                     /locus_tag="MJF80_000286"
     CDS             27239..27979
                     /locus_tag="MJF80_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBZ0278968.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGILSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            27984..28976
                     /locus_tag="MJF80_000287"
     CDS             27984..28976
                     /locus_tag="MJF80_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBZ0278969.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYITTGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(29164..30045)
                     /locus_tag="MJF80_000288"
     CDS             complement(29164..30045)
                     /locus_tag="MJF80_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBZ0278970.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            30318..31706
                     /locus_tag="MJF80_000289"
     CDS             30318..31706
                     /locus_tag="MJF80_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278971.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYANGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            31734..32687
                     /locus_tag="MJF80_000290"
     CDS             31734..32687
                     /locus_tag="MJF80_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBZ0278972.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            32747..32971
                     /locus_tag="MJF80_000291"
     CDS             32747..32971
                     /locus_tag="MJF80_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278973.1"
                     /translation="MNEKSSFKQIEIILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            32964..33476
                     /locus_tag="MJF80_000292"
     CDS             32964..33476
                     /locus_tag="MJF80_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBZ0278974.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            33508..34362
                     /locus_tag="MJF80_000293"
     CDS             33508..34362
                     /locus_tag="MJF80_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBZ0278975.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            34896..35690
                     /locus_tag="MJF80_000294"
     CDS             34896..35690
                     /locus_tag="MJF80_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0278976.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            35794..36600
                     /locus_tag="MJF80_000295"
     CDS             35794..36600
                     /locus_tag="MJF80_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBZ0278977.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            36662..37183
                     /locus_tag="MJF80_000296"
     CDS             36662..37183
                     /locus_tag="MJF80_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBZ0278978.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            37598..39685
                     /locus_tag="MJF80_000297"
     CDS             37598..39685
                     /locus_tag="MJF80_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBZ0278979.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMDDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            39700..39987
                     /locus_tag="MJF80_000298"
     CDS             39700..39987
                     /locus_tag="MJF80_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0278980.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            40014..41285
                     /locus_tag="MJF80_000299"
     CDS             40014..41285
                     /locus_tag="MJF80_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBZ0278981.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            41343..41942
                     /locus_tag="MJF80_000300"
     CDS             41343..41942
                     /locus_tag="MJF80_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBZ0278982.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQEYLKWHPAGSLGKSIREGI"
     gene            41947..42852
                     /gene="dapA"
                     /locus_tag="MJF80_000301"
     CDS             41947..42852
                     /gene="dapA"
                     /locus_tag="MJF80_000301"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBZ0278983.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKSQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            42958..43590
                     /locus_tag="MJF80_000302"
     CDS             42958..43590
                     /locus_tag="MJF80_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBZ0278984.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNVRKI"
     gene            44067..46574
                     /locus_tag="MJF80_000303"
     CDS             44067..46574
                     /locus_tag="MJF80_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBZ0278985.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            46916..49078
                     /locus_tag="MJF80_000304"
     CDS             46916..49078
                     /locus_tag="MJF80_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBZ0278986.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            49083..49700
                     /locus_tag="MJF80_000305"
     CDS             49083..49700
                     /locus_tag="MJF80_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0278987.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLDLSEQADRVIKL"
     gene            49889..50233
                     /locus_tag="MJF80_000306"
     CDS             49889..50233
                     /locus_tag="MJF80_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBZ0278988.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            50803..51669
                     /locus_tag="MJF80_000307"
     CDS             50803..51669
                     /locus_tag="MJF80_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBZ0278989.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPINK
                     ESGKPCINLQPDFKCSVHNSLIKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESMMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            51782..52186
                     /locus_tag="MJF80_000308"
     CDS             51782..52186
                     /locus_tag="MJF80_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBZ0278990.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            52350..52799
                     /locus_tag="MJF80_000309"
     CDS             52350..52799
                     /locus_tag="MJF80_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0278991.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(52955..53332)
                     /locus_tag="MJF80_000310"
     CDS             complement(52955..53332)
                     /locus_tag="MJF80_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBZ0278992.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            53594..54511
                     /locus_tag="MJF80_000311"
     CDS             53594..54511
                     /locus_tag="MJF80_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBZ0278993.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            54995..56413
                     /locus_tag="MJF80_000312"
     CDS             54995..56413
                     /locus_tag="MJF80_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBZ0278994.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            56489..57301
                     /locus_tag="MJF80_000313"
     CDS             56489..57301
                     /locus_tag="MJF80_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0278995.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
BASE COUNT        22473 a         6172 c        10113 g        18671 t
ORIGIN      
        1 tggaaatttt gagcttagga gaaaagatta aaaaattaag aaaagaaaaa aacatgacat
       61 taaaagaatt agctggagat aggataacag cagcacaaat aagccatata gagagggata
      121 aatctcatac tagttatgaa ctgttggaat atctaggcga taagctagat gtaagtgtag
      181 aatacttatt agagaccaag gaaatgcaat caaaaaagat aactgataat ttaatactac
      241 agagcgaaat ttatataaaa gaaaatgaat taaacaaggc tgaggaccaa ataaaagaag
      301 ttttagatat atgtaagaga tacgatttaa tggagaacta tggcagatgt aattttttaa
      361 tggctaatat taatttgaaa aaatctaatt ataatgatgc tatagaaaac tttgaaaagg
      421 cattgtactt ctttataaaa aatagtgata gtgaaaatat acttaagtgt tatttaaaca
      481 tagggaaaat atatatgcag gaagactttt ataaaggtgc tataagccac tttaattttg
      541 cagaggaaat tttgtcagag aataaatttg aggatgttaa catacacaag gaactatata
      601 gtaagatggc atattgttac ataaaactag ataatccaaa tatgtcattg aagtacatag
      661 aaaagataaa tgaaatagat aatagaaatg atagaaaaga agatgtcgat attttggtgt
      721 taaaagcaaa taatatgctt gagattggaa aatatgaaga atcaagagat tactttaaaa
      781 aagcactaaa aatacttgag agtgaggata ataaaactgg attagcaaat gtgtatttaa
      841 caatttgtga tgtttacaga aaaataggtg aaacagatag agtacttgaa tattcacaaa
      901 aggtatatga cataaagaaa aatgatgaag atgaatacat gatgtcagga ttatttaaga
      961 taatagaagc atatatagat aaagaagatt atgatttagc tagaaaatat tgtaagatag
     1021 ccttagcttc atcaataaaa aataaaaata aattcaatga atataaagca ttgagattct
     1081 atgctaatat gtataaaaat cagaatgaag taactcttgc aattgaatat ctgattaaat
     1141 gcataaaaat agtatcagat ttaggcaaca atagaatatt ggccaattta tatatagact
     1201 taggtcaatt atattcaaat atatcaaaag aaaaagagtt agaatattat caaaaaggtg
     1261 tatttatgta caaaaattta gaaataatgt agggtaaata aacctataag ggtatagaaa
     1321 caatctataa caagcaagat aatatctatg ataaaatata aattgtatga atatttttaa
     1381 ggagatgaat cagacaatga gtataaaaat agatgcgaga gggcttgctt gtcctaaacc
     1441 agttataaat acaaaaagag aattggataa cctagaagaa ggtgttgtag ttacaatagt
     1501 cgataatgaa acagctaagg agaatatatt aaaacttgct aaatctttaa gttgtgaagc
     1561 aaatattgtt gacgaaaaag agaattttat atctatagaa attagaaaag gtaaaaatat
     1621 actagataaa caaaatgttg tagataataa agaaagtgaa ttagacaata catgtatatt
     1681 tatatcttca gataagatgg gacttggaaa tgacgaatta ggaaaagtgt taataaaagg
     1741 atttatatat acattaacag aatctaaacc ttatcctaag tatgtacttt tagttaatgg
     1801 aggagtaaaa ttaagtgctg aaaatgaaga aaccatagaa aatctaaaaa tacttgagaa
     1861 tatgggtgta gaagttttat cttgtggtac ttgtttagat tattataatt taaaagacaa
     1921 attacaagta ggtacagtta caaatatgta tactatagtt gagacattaa agaatgcatc
     1981 aaataccatt tctatataat gtatgtttgt ttaataatat aaaattaata ataaagatta
     2041 actcaaatag atagtcagaa aggattaagt aatgaatcaa atgtatatag tgtcatttaa
     2101 ttcgacacat catgcgataa gatcagaaaa gttatttggt gaaaatagtc taaaagtaat
     2161 ggctcttcca acaccaagag aaataacagc aagttgtgga atatcaatta aattttcttt
     2221 tgaagatatg gaaaagataa aaactatttt agttgaaaat aatgtagata taaaaggaat
     2281 ttattgtata agtaaattag aaaatggctc aaaagaagta gagaagttag attaggaggc
     2341 taattattta tgccaatgga tttaaaaata ggtgatattg ttgaattaaa aaagcaacat
     2401 gcatgtgggt gtaaagagtt tgaaatcata agaacgggaa tggatataaa gataaaatgt
     2461 actaaatgtt caaggctaat aatgcttgat agagagacct tagaaaagag agtaaaaaag
     2521 ctaataaaaa agtgataaaa atttaacact taaaaaatgt caaataccgt atattaatat
     2581 ccaaaatttt ggtataataa gaattgaaga aaacattttc atgggggtgc tttaattatg
     2641 gcagttaaat atgcaaaaag aatgcaagga ttacaaggat cagaaatacg tgagctttta
     2701 aaacttactc aacaaccaca aataatatct tttgctggtg gaatgccggc tccagagtta
     2761 tttccagttg aggaaatgaa aaaagtatca gttgcagtgc ttgaagaaaa tggaagatca
     2821 gctatgcaat atactacaac agaaggatat gaaccattaa gagaaaaaat agcagctaga
     2881 atgaatgata aaaacaaaac taatgtaaac aaagatgata tattagttac aagtggttct
     2941 caacaaggtc tagattttgc aggaaaagta tttattgatg agggtgatgt aattttatgt
     3001 gaaagtccat cttacatagg agctataaac gcatttaaat cttatcaacc taaattcata
     3061 gatgttccaa cagattctga tggaatgata atggaagaat tagaaaagat actagaaaca
     3121 actgatagaa taaaaatgat ttatgtaata cctgacttcc aaaatccaac aggaagaact
     3181 tggccacttg aaagacgtaa aaaattcatg gaaatagtaa ataaatttga aataccagta
     3241 atagaagata atccatatgg agatttaaga tttgaaggag agactttacc atctttaaaa
     3301 tcaatggata caaaaggatt agtaatattc ttaggaactt tctctaaaat attctgccca
     3361 ggatacagat taggatggac ttgtgcttct ccagaaattt tatctaagtt taactttgca
     3421 aaacaaggtg cagacttaca agcatcaact atatctcaaa tggaagtaag caaatttatg
     3481 gatatgtatg acctagatgc acatgttgac aaaataaaag cagtttatgt taaacgtaga
     3541 gatgtaatgc ttaagactat ggaagaagag ttcccagaag gattagtatt tactcatcca
     3601 gaaggtggat tatttacatg ggtagagctt ccaagtaatt taaatgctaa agaattaatg
     3661 ccaaaatgtt tagataagaa tgttgcttat gttgctggag gaggattctt ccctaatggt
     3721 ggtagagaaa atactttcag acttaactat tcaaatatgc cagaagaaaa aataatagaa
     3781 ggtataaaaa atatagcagc tgtattaaaa gaagctatgg gtgtagaagc ttaattaaaa
     3841 ataataactt atatataata caagggatgg ttgttgccat cccttgtttg tttgaaaagt
     3901 atataattca ccttatacta aactttgtcc aaaatttaaa ttgacagaaa taagaataaa
     3961 tgataaaatg tgtaggtatg agattttagt ctcatttctc tgctctaaga gtgtagagcc
     4021 gttaagtcca aagggaggtg aattatagtg agaaattatg aattagttta tgtggtaaag
     4081 ccaaactctg atgaagaagt aagagaggct atactaaaca aagttaagga agttgttgca
     4141 actgacggag aaatagttaa agttgatact tggggaacta aaaaattagc ttatccaata
     4201 gctaagttta cagaaggttt ctatgtatta gttaacttca aatcagcagt agacgttcct
     4261 aaagagatag acagaaactt aaagataaat gaaaacgtaa taagacacat gatagttgtt
     4321 gcttaataac tggttttatt ataaaaaata ggtggtgttt ttatgaatca agttgtatta
     4381 gtgggaaggt tgactagaga ccctgaatta agatacatcc caggtacagg aacaccagtg
     4441 gcatccttta caatagccat tgatagagac tatgtaaaaa aagatggatc taaagaaact
     4501 gattttatac ctgttgaagt aatgggtaaa tctgctgaat tttgtgctaa ctacataact
     4561 aaaggtagat tagtagcact tcaaggatct ataagagtag acaattatca gacacaatca
     4621 ggtgaaaaga gaacttttac aaaagtaagt actagatcag tacaagcttt ggatagtaag
     4681 aataaatcgg aaaattcata tagagagagt gctccagctt ttgagccaag ctttgaacct
     4741 caaggtttag acccacaagg tttccaagct atagatgatg acgatatacc attttaaaaa
     4801 ggagggaaaa aagtcatgat gaataaaaaa agacgtaaga aaaaaagagt ttgtcaattt
     4861 tgtgctgaca aaaacgctaa aatagattac aaaagtactc aaagattaca aaagtatata
     4921 actgaaagag gaaagatatt accaagaaga atatctggaa cttgtgctaa gcatcaaaga
     4981 gaattaacag ttgctataaa aagagcgaga aatatagcac ttttaccata tacattagac
     5041 taaaaaaatg agccttgtgc tcatttttta ttatatgtat ttatatatct aaatgaggcg
     5101 agctataaag ctcgtttttt tgtttatata cttaaatata ttattattta tgatataata
     5161 ttatatatta aggagggttg taaacgtgaa aatagataaa ggttctgaga taacgagaaa
     5221 cataaagata atagaatgga tgaaaacaga aatattaatg agtgtaagtg atttgtttaa
     5281 tttattgttt aaaggggtaa aaccattaga tgaagcaatt caagatactt tagcaaatat
     5341 aataatgata acatatctct tagcaaagag attaggaatt agttttagag atgtggatta
     5401 taaagtcaag gaaaaaataa aaattggtat agatgaggac catagcgttg aaagatggta
     5461 tggagattta tctaatttaa aaaaacatat ggataatagg gagtgatatt aaattgaata
     5521 ataaaattag actatctaag gcaatgtcta taatagtttt agctataata atcgctttag
     5581 taatagccta tattccaatg ctaggaatat ttagtatgtt agcagctgtt ccatatgtaa
     5641 ttataggtgc tattactgat agaaagtatt ctcttatatc tattttggtt acattttgtg
     5701 tactgattct atttgcagac atttcttatg cattaaatat atgtataatg tattctttac
     5761 caggtatagt aataggaaag atgctaaaga gaagctttga acaagaggac agtaataaat
     5821 tcgaaccgat atatggtgga accataatat ttgtactttc aatgcttgta tatttttttg
     5881 tattaaagac atttttaaat gtgaatctat tagatgaagt ttctaagatg atatcagaaa
     5941 ttgtaaatat tcaaaaaaat agtttttctg caactgaact taaagtgttt gataaaatga
     6001 agccagatga gattgtaagt tattttacaa atatgctacc aatgatgtta tttttacaag
     6061 ggttgttatc agcgtttgtc acatattatt tgagtgtatt ttttataaaa aggattacaa
     6121 agatgaatat aagatttcct aagtttgctg atttttattt accgggaaat gctatactca
     6181 caactttctt actttattta ttagtcttat ttattcaaat tattggttca aaattatata
     6241 cagaattaat aatgactaat ttacagctag tttttaattt aatgtttgta attcaaggta
     6301 tagcagtttg catttatttc ttaaagaaat ggataagaca gggaccaaat aaaattatgt
     6361 ttttttcagg gataatttta tgtttatttg gatttatggg aatatctttt gttggtatgg
     6421 tagatagtat aattgacttt agaaaggtaa gaagctataa atccacttag gaggatagaa
     6481 atgagtaata aacaaacctt taaattaaat atgccagaaa taaatttata tataattgtt
     6541 attggtattt ctagtataat tttgctttat tacaacttgt atgtagggtg tttatttttc
     6601 tgtatttttg tatatatggt ttttcacaat tggagaacta ctaatattag acgtcatgaa
     6661 tggacagagt atattcaaaa tttatcttta gatatagatg aaacaactaa aaaagccata
     6721 ataaatcttc caataccatt atgtatttta gaatttgatg gaaatatatc ctggtataat
     6781 ggaaagtttt atgatatgat tggtcaaaaa gatttacttg ataaaaatat agaggatata
     6841 gttaaaaatc ttaatttaag aaaagtatta aatgaaaata aagagatgta cacagaaata
     6901 aactataaag aaaaagaata tacaataatt tataatgtta taaaaaatga tcaagaaaaa
     6961 aatcctaaat atttgatgat attatactgg atagacaaga ctgaatattt aaaagtaaaa
     7021 caaaattatg atgatgaaaa aaatgcaatg atgctaatac aagtagatgg atatgatgaa
     7081 gtattaaaaa gtgcagcaga agataagaga gctttaatta atgttgaagt agagaaaata
     7141 ctttctgctt tagaattaaa ttcaaatggt gcattgagaa gaacttcaaa agacaaattc
     7201 ttcttggtaa tgcataaaaa agagttaaaa aaattggaag ctgagaagtt ttctatttta
     7261 gatacaataa gacatattga ttatggaaat aatcttcctg ttactataag tataggaatt
     7321 ggtatagatg gagatacatt aaatgagaac ctaaaacttg ctacgggtgc tctcgattta
     7381 gcacttggta gaggtggtga ccaggctgtt gtaaaaacaa aagataaatt tgtgttttat
     7441 ggaggaaaat ctaaagctgt agaaaagaaa accaaagtaa aatccagatt aataggtcat
     7501 gctttaagag aggttataca acaaagtgac caagtttata taatgggaca taaatatcct
     7561 gatatggatg ctatgggagc agcagttggt gtttatgata tatgtaaatc ctgtaacaaa
     7621 actgcaaaca tagttttaca atctgtaaat gaatctatag aaatatttat aaataaaata
     7681 aatgaaaata attactataa aaagttgttt attggaaaag aggaagctat tgacaactgc
     7741 actaaaaata cattagttgt agtagttgat actcatagac caaattatac tgaatgtgaa
     7801 gaactgttaa agctatcaga gaaggtagta gttatagacc atcatagaag aggtgtagag
     7861 tttataaatg atgcagtgct tttattccat gagatatatg tatcctcaac gtgtgaaatg
     7921 gttacagagt tagttcagta tatggatgaa gatgtaacaa taaataaact tacagctgaa
     7981 gggcttttag caggtataag tctagatact aaaaactttg cattcaaaac aggtgttaga
     8041 acttttgaag ctgcttctta ccttagaaaa gttggagcag atactataga ggtaaagaaa
     8101 ttcttcaatt ctgatgtaaa agatttcata ataaaagctg aaataattca aagtactaaa
     8161 ataattaata atagaatatg tttagcatat tcaagtactg agatagatag tataaatgtt
     8221 ataatagctc aaactgctga tgagttatta aatatcaaag aagtagaagc ttcctttgta
     8281 ttaggtgaaa aagatgatac tatatttata agtgcgaggt ctttaggaca aataaatgta
     8341 catgtactta tggaaaaatt aggaggcggg ggacacatag atatagctgg agcacagtta
     8401 aaaaatgttt ctctaaaaga agcttataag atggtaaata aaataataga ggagtatttg
     8461 gaggaggaag aatagatgaa agttatatta ttaaaagacg taaaaggaac tggtaaaaag
     8521 ggagaaatga aagaagtaag tgatggatat gcaagaaatt tcttatttcc taaaaagatg
     8581 gctgtgcaag cagatagtgt agcaataaaa gaattaaatg aaaagaataa atctaaagaa
     8641 ataaaagcac aaaaagagta tgaagaagca gttttattag gtaagcaaat ggaagaaatt
     8701 aatatagaaa tatattcaaa agcaggagaa ggtggaagat tatttggttc aataacttct
     8761 aaagaaattg ctgagcaatt aaagaaacaa aaagacatag atgtagataa aagaaaaata
     8821 ttattagatg aaccaataag atctttaggt tcaactttcg tagaaataaa aatacatcaa
     8881 aaagtaacta ctaaaataag agtggatgta aaagaaaaac agtaataact agaggtgatt
     8941 aagatggaag atatgacgag aattcctcct catagtgtag aatcagaaca atcaatattg
     9001 ggttctatac ttctagataa agatgctata ataacggtta cagaaactat aaagcctgat
     9061 gatttctata aagaggctca caaaataata tatgaatgta tgataacttt aagtaataaa
     9121 ggtgagccta tagacttgat aacattaaca gaagaactaa gaaaacaagg gcatcttaat
     9181 gatattggag gaatcagtta tattacaagt ctttctacta tagtaccgac gacttcaaat
     9241 gtaaaatact atgctgatat agtaaaagaa aagtctgtac ttagaaagct tataaaagct
     9301 tctaatgaaa taataaatct aggatatagt ggagcaacta agattgaaga tgttttagaa
     9361 caagctgaaa agagtatatt tgatatatca caggagaaaa ctagtgatga ttttaaatct
     9421 ataaacttag ttttgatgga tgcctatgat atgatagaaa aattatacac taacaagagt
     9481 gatgtcactg gtataactac tggttttaaa gatttaaaca aaaaaataaa tggtcttcaa
     9541 aggactgatt taatattaat tgcagctaga ccagcaatgg gtaaaactgc cttttcttta
     9601 aacttggttc aaaatgcagc cttaaaaggt gatgcctctg tagctgtatt tagtttagag
     9661 atgtctaagg aacaattagt acaacgtatg ttatcttcac aatctagtgt agaattaaaa
     9721 aaaataaaga ctggaacact taatgataat gattggccta gaattataga tgctatggca
     9781 gtattatcag atgccaaaat acacatagat gatactcctg gtataaaaat atcagaacta
     9841 aggtcaaagt gtagaaagtt aaaaatagaa aaaggactag atttagtgct tattgattat
     9901 cttcaactta tggaaggtga aggtaataat gaaagcagac aacaagagat atctaagata
     9961 tcaaggtcac taaaaatatt agcaaaagaa ttaaactgtc ctgtagtagc actatctcag
    10021 ttatcacgtg caccagaaca aagagcagac catagaccta tgttatctga tttaagagag
    10081 tctggagcaa tagaacagga tgcagatata gttatgttct tatacaggga cgaatattat
    10141 catgctgatt ctgagagtaa aaatatagga gaagtaataa tagcaaaaaa tagacacggt
    10201 gaaactggtt ctgtggaact tgtttggctt ggtgaagttc aaagatttgg ggataagttg
    10261 agagacctat aatctaacga atatccacag aacgtccttt ctttctacag gaaaaatgtg
    10321 gatacatgtc actaaatatg tccaaacatt gaaatttagc taaaaaacta tccacactat
    10381 ccacagatgg cctgttgata agtgtgaata agtagataaa gcaagctttt ggcttgcttt
    10441 atctgtgtat aaatattaat tattaggagg aataatatgg aaaaaataag cttaaaatat
    10501 atatatccca atataattaa agtacttgat gaaataaact tatttagagt aattgacaat
    10561 aatttaagag aatctattgt tgtttatgct aataatgtgg ataatcagta tcatataaac
    10621 atgactaata caaactttgg aaatataatt aacatatgta aattagaaaa acttttagat
    10681 gtggataaat ttatggaaaa agttataaaa tatgaaaaag aaataataga gaaagaagag
    10741 ttttcaaaaa tagaagaata tatgttaaat attggagaat actaaaaaag ttgaaaaaaa
    10801 tacgaatgtt tgatagaata agtaagtaaa gtgttcgaga gtatataaaa tatagaggtg
    10861 aattacatga aaacagttgc aattgttggt tctcaatggg gagatgaagg taaaggtaaa
    10921 gttatagatt atcttgctac tcaagcagat gtagtagtaa gagggcaagg tggaaataat
    10981 gcaggacata cattagttgt agaaggtaaa aaatacgcat tacatctaat tccatctgga
    11041 gttttaaatc caaatacagt gaatataatc ggaaatggaa tagtgtttga ccctaaagga
    11101 tttttagaag aattggaaat gtttaaaacg gataatataa gtacagaaaa tataaaaata
    11161 agtgatagag cacatgtcat attcccatat cataaagaac tagatgcatt gtcagaagaa
    11221 gccagaggcg atttaaagat aggtacaaca aaaaaaggta ttggaccttg ttatatggat
    11281 aaaacagaga gatctggtat tagaatatgt gacttaatgg ataaagataa atttgctata
    11341 aaattaaaag ctcaaataga tgctaaaaat gaaatagtta aaaatatata tggaaaagaa
    11401 gaattatttg attttgaaac tatatataac gaatatctag gatatgcaga gcaaataaga
    11461 aaatatgtgg cagatacgtc tgttattgta tatgatgcag taagagcagg taaaaaagta
    11521 ttgtttgaag gagcacaagg gactttatta gatttagatt taggaactta tccatttgtt
    11581 acatcttctc atccaacatc tggtggattt gcaattggtg caggtatagg gccaaatatg
    11641 ataaaagacg ttgttggtat agtaaaagca tatactacaa gagttggaga aggaccgttt
    11701 gttacagagc aaataaatga aactggagat aagataagag aacaaggaca tgaatttggg
    11761 gtaactactg gtagaccaag aagatgtggg tggtttgatg cagttatagt taagtatgca
    11821 gctagagtta atggattgac tagtatttca tttatgttac ttgatgtatt aactggattt
    11881 gataagataa aagtttgtac gtcttataaa atgggcgata agataataac ggatttccca
    11941 gcttcattag atgatttagc aaaatgtgaa cctgtatatg aagaattaga tggatggaat
    12001 gaagatataa ctcaaataga taactttgat gaccttccag aaaatgcaaa gaaatatgta
    12061 gcaaaaatag aagaactagt tggagtaagt gtagatatgg tttctgttgg tccaaataga
    12121 gctcaaacta taataagaag aaatatattt gcttaatatt ttatcaatta aataatttaa
    12181 aatagttgaa gaagagtatc tcaagataat gtttatagtt attagagatg ctcttttata
    12241 tttgacttac attgattttt tattaagtta aagctactta tacatattct aactgaaatt
    12301 aaagttgaat ttatgtagat acatataggt tttttaaaaa gaattttgtt gaaaataaat
    12361 ggctaaagat gattttaatt atattctaaa aaaagtaaaa aaaactattg actaaaaatt
    12421 atatagacgc tataatataa cttgtgagtg aaagaaacga cgaaaaaagt cgaaaaaatg
    12481 tggtctatta gctcagtcgg tagagcacct gacttttaat cagggtgtcc cgcgttcgag
    12541 tcgcggatag atcaccaaaa tatggctcgt tggtcaagag gttaagacac cgccctttca
    12601 cggcggtaac aggggttcaa ttcccctacg agtcaccagt atgggactat agctcagctg
    12661 ggagagcacc tgccttacaa gcagggggtc acaggttcga gccctgttag tcccaccaaa
    12721 ttttgaatta gagataaata ctagattata tgctggtgtg gctcaacggt agagcagctg
    12781 acttgtaatc agcaggttgt aggttcgatt cctatcacca gctccacaaa gtacataaat
    12841 tcaaattaat ataattttct atgttacagg atacttttaa gtatcttttt ttaatttcat
    12901 ttttattgta ttaaaaatga aattaaacat acaacaaaat tttgaattac ttgcaatgtg
    12961 atagttttaa atataaaatt tagaatcatt ctagcataat attatgtaat atagcaaatt
    13021 taatttatag tatcaataaa tatagactac taagaatgta aatgatttgt tagattaagg
    13081 tacattagat atgtaaatag aaaaataaaa agtatgtttt aatttatgtg atataagttt
    13141 taaacagaga agcaagtttg ctcttacctc tctatttaaa aataaaaaaa tgttttttaa
    13201 gggctatttt atttaaatct ttcccatctc aaatcatctc cagtaaattc taaaatccta
    13261 aacttatcta atattctaga gagagttctt tgagtgtaag tatttcctat ttgagatggt
    13321 gttaagttgg ttgaaataat gattttttta ccagcaacaa gtcttgtatt tacaatattg
    13381 aagatttctc cactagtaaa agaattatta agttcagttc caaggtcatc tattattaat
    13441 aaatcacaat caaacaagtt tttataatta tcttcactta tttgattatt tgtgtctctt
    13501 ctaaatttat aatcttcaag aatatctaaa attctaaaag aagtttgata gattacaaca
    13561 ttgcctttat ctaacaattc ctttgcaata caattgcaca tataggtctt tcctagtcca
    13621 gtagaaccat aaaacagtag attttcacta ttatccttct taaaatcatg aacaaaattt
    13681 tcgcagatac ttaaattatt tagcatattt tctctaggag atatttcacc atcagagcct
    13741 ttcttaggag aaaatatatt caagttaaaa ttcgaaaaat tttcctgact taaaatccta
    13801 tctaaattag acattttata agcttcattt acaatttctt gtttgaggca agaacatttt
    13861 tctccattag gtaaaaatcc tttatcttta caagaaatac attgaaactt tatttccaag
    13921 taatcaagag gtatgttact ttcagctaaa agtctttctt tctttacttt taaagattct
    13981 atattctctt tagtcttctt tacaatttca tctttagact taggatttaa aagtactatc
    14041 ttagctaaac tgagacctat tttagaaatt tcatcatcta tagattttat ctcagggatt
    14101 ctattatata cctcattttt tctatgttct aataagagtt cattatcatc tcttctctta
    14161 gcatatttag caagtatttt tcttatttta tcctcattca tttaatcacc tacttaaact
    14221 tttctttttg acttttttta ataatctcat cgagctcatc agaggtatac tgagtgaatg
    14281 tttcgttgaa attatgaaac ttagtttttt tgtaagtatt gttgttttga gtagtattaa
    14341 tctgcttctt attaatattc tccttaacta tacgttcttc ctctttttgc tttaaatcat
    14401 ttaaattttt tatatttttt tcattccaat ttttaattat accatttata taagatatac
    14461 ttgggtttga aatattctta gacttagaac atgcatttat tatcaaatct atatccatat
    14521 taaatttatc aaaccaggtg tccataagtt ctttttctga ttttgatggt tgtcttgaaa
    14581 atcccaattc attgaaaatc gttttatata aaatatatcg ttcacttctt accaaaaagc
    14641 tttcttcaac atcttttggc gtatataaat ttgaatcata ccaatttcta ataatgcctt
    14701 caatgtattt aactggcttt gatgtaccag ttttatcttt gacgtattca tatgcgcaaa
    14761 gtatcatatc ggggctcata ttgtatttat tcattatatc cataatactc atcttttcac
    14821 ttgggtcgag atatcgccca actattttat ttatagaatt aaacattttt ctaatacttg
    14881 gattttcaga agtagataca actttatctg tattagattt tatagatgaa ttattgctta
    14941 atatattctc aatatagaaa ttttttaaat ctaaaaattc tattgaataa ttaaaattat
    15001 catattcttc attgtcatgt atttttataa tcttcttttc ttcccagaat ttccaagcag
    15061 ataacacatc agacaatggt atatttaggt ttttggctat tgaattattg tcgaacttag
    15121 gattagaagt aatatcacat gcttgtctat atcctaaaag ataaactttt acgtataacc
    15181 cgtccgccat tggcataaat atatctataa agatattggg aatagtgatt tcacctaaat
    15241 ctatttcatt gctttcttta aaaaacattt tatcacctca gattaaatta tatcattttt
    15301 tatattatat ttgtctgtaa acaaataaaa ggtcacaaga ttagcatgta aatattttct
    15361 aaaagtattc attttgaaaa ggaggagata ttttggttat aaaattcaac caaaaagtaa
    15421 gaagaacatt atcagtagcg attctattta tggtattaat aataggttgc tttatagaaa
    15481 aggatgacag tttagaaaca tacaaagaaa aaactaattc aaaaataaat cagtataata
    15541 tggatgttat atttgatgat gagactaaga ggttaatgtg caatcagaat gttgactaca
    15601 taaataatac aaaatcaaac atagataaaa tatatttcca tatatatccg aatgcttttt
    15661 ccaagaaaga ttttgctcct tttgaaaaaa gtgaaatgac aagggcttat cctaatggat
    15721 ttaacgaagg atatatagat ataaagaata tattaaataa taatagtaaa atggagtata
    15781 aaattaaagg agacaaaaat gacatattag aaatagaatt agggaaagag ttaaaaccaa
    15841 atgaaagaat gtcattagat ataaaatata acgtaaaaat accaaattca attggaagat
    15901 ttggatatgg agaaaatact ataaatgtta ctaattggtt tcctatagct tgtgtaaatg
    15961 atgaaagggg atggaattta aagagttatg aaactattgg cgacccattt tatagtgaga
    16021 ctagcgattt tcatgtgaaa ctattgattc caaataaata taaaattgct catacaggaa
    16081 aacttattga tgataaacat gataataaga aaatgctata tgaaatagaa gctgaaaacg
    16141 ttagagattt tgcatttata ctaagtagta aatttgatgt aaacaaagta gattcaaagg
    16201 gagtagctat caatacatat aatttaaata aaaagctttc taaaaaagca actgaagtgg
    16261 caaaagattc aataaatata tttagtgaat tatttggaaa gtatccttac aaagaatttt
    16321 ctgttgtagc aagtgacttt tttatagggg ggatggaata ccctatgctt gttatgatag
    16381 accaaagttt atataatgaa aaaaatgaat ttttactaga atatgttatt gcccatgaga
    16441 cagcacatca atggtggtat tctgctgttg gaaatgatga aataagtgaa ccctggttag
    16501 atgaagcact tactgaatat tctacaatag tttattttga acaaaagtat ggtaaagata
    16561 tggcaaacaa gcttataaaa actatggaga tacagacaaa aagttatttg agtgaaaata
    16621 tatttaagcc agcaaatgag tataaaaatt ctactgaata cagcttgaat gtttatacaa
    16681 aaggcgcaat agcatttaat gaaataagaa aagaagttgg agataaagta ttctttgaga
    16741 ctttaaatga gtactataat aaatataagc ataaaaatgc taatggaaga gcttttgtgg
    16801 aactatggaa tagtaaaggt gtagatataa ataagattat aagtgaatgt aagtaaatag
    16861 tgaatgtaag taaattttgt gcagagtatt aaattttatt taatactctg tttttttggt
    16921 ataatataat ttataaggca gaaaaagagg tgaaggaatg ctaaagtatt gttcagtagg
    16981 gagtggtagt agtggtaatt gccactatat agggtataaa aatactaata tactagtaga
    17041 tgcaggatta agtggcaaga gaataactac aggtcttaaa gacattggtg tagatgcaga
    17101 taaattaaaa ggaatattta taacacatga gcatgttgac catataaaag gagcaggtat
    17161 attatcaaga aagtttgata taccagtatt tgctaatata aaaacatggt gttctatgaa
    17221 ggacaaatta ggtgacatca aagataaaaa tatgaaagta tttgaaaatg ataaaaccta
    17281 ctcattaggt gatatcatag taagaccttt ttcaatagaa catgatgcgt ctgacccagt
    17341 tggatataat ttttatacag agagtgatga aaaaatgtct atagctactg atataggatg
    17401 tataactcaa aatataaaaa aacatctata taaatctaaa ttagtagtat tggaatcaaa
    17461 ttatgaccct aatatgctta tgatgggttc atatacatat gctttaaaga aaagagtaat
    17521 gtcaagtact ggtcatttat ccaatgaaga tgcagctaat ttctgcgttg aacttattaa
    17581 tgaaggtaca gaatctatat tattagcaca tttaagcaaa gaaaataatt ttcctgagtt
    17641 agcgtatgag acttcaaaag gagtattagc atcaaatgat atagtagttg gacaagatgt
    17701 aaaactagat gtactatcac gaaatgatgt ttctaatgtt tatgagatga agtagaggtg
    17761 aataactttg ctaaagagaa attttgaaaa tgttgaggat aataaaattt tatactctat
    17821 aaagcagggt atgattttag cgatacctgc tattatgact ggttcaactg ctcttgtaat
    17881 tttgaatctt ccaataaagg cttatcaaga atatttatca ggcttgttta atggagaggt
    17941 tacaaatatt cttaacttta taaatgattc tactttagga attatttcat taataatatt
    18001 attaacaata agctatagtt atggaaaaat atatgggagc aagtatacag tacttgttcc
    18061 tatagttgca atgtgctctt ttttagtatt ttcacatggt aatgagctta attcttatat
    18121 agaaatattt aaaacaaagt ggttatttac atctataatt gtttctatga catcttctgt
    18181 attgtttgta aaacttacag aatcttttgt tacaagtgta aagtttcata cagaaggggc
    18241 tgatgcagac tttaatatgg tagtttcggc tatagttcct ttcataatag tagtattttt
    18301 gttttctatt tttagagtgg taatgataag tctaattggc agttcaaact ttcaagatat
    18361 attttataat ttattttcaa atgtttttaa taaaatggga acaaacctta tgagtgctct
    18421 gttgtttatt tttttaatgc actttatgtg gttttttggg attcatggta gtaatgtatt
    18481 agataccgtc gcaaaaaatt tatttgaaaa tagtatggca attaacataa atttagttaa
    18541 taataatcaa ttaccaacag aaatttttac taagaccttt tttgatacaa tggttttatt
    18601 gggaggatgt ggctctttac tatgtttagt aattgcaatt tttcttagtg aaaaacgtat
    18661 aaatgtaaga aagttagcta aaattgcttc aataccagct ctttttaata tcaatgagat
    18721 gctagttttc gggataccaa ttgtttttaa ctatattatg tttgtacctt ttgtaattac
    18781 tcctataatc ttaaccgtta caagttatgt ggctatgtct acaggtattg taccatgtac
    18841 agtttcagct gtagaatgga cctcacctat atttttaagt ggatatatgt ctacaggttc
    18901 aataagaggt agtttgttgc aagtatttaa tttatgtgtt ggtgttatga tttatattcc
    18961 gtttataaaa atgtcgcaaa atagatatac atatatgttt aaaaataata tagaaaattt
    19021 aacaaactta ttaaaaaggt atgaattaac aggtgaacag cctaatcttc taaaccataa
    19081 tggtaaaata ggttcaattt caaaaatgtt ggcatcagac ttgaagtttg ctatgaaaag
    19141 aggagaaata gagctttttt atcaaccaca agtcaattat aatggaaatg ttgtgggagc
    19201 agagggatta cttagatgga aacattcaat aggagggttc atttatccac cactagttat
    19261 aattttggct aaagaagaag ggttcttaga tgagcttgga gagtatatta ttaataaagc
    19321 atgtattgat ttaaagaaga gtgaaaagct attgagaaat cctgttaagt tctctgtaaa
    19381 tatatctcca gaccaacttg atgaccctaa gttaccagag caaataaaaa atatcattag
    19441 tagtaatgat ataaatccta atatgttagg tatagaaata acagagcaaa ttgcattatc
    19501 aggttcacaa ataattattg aacgtatcaa tgcgatacat aacttgggaa taaagttaat
    19561 tatggatgat tttgggatgg ggcatagctc tcttatatat cttcaaaata ataactttga
    19621 tatagttaaa ttggatggtt cattagttaa agagatactt acaaataaaa gaagtagtga
    19681 aattataatg actattgtaa atctttcaaa aaatttagat tttgatatag ttgttgagta
    19741 tgttgaaaat atagaccaaa gggataagtt atatggttta ggatgtgaaa tatatcaagg
    19801 atattattat agcaaagcaa taccttttga agagtttgta gaatatgcaa atcaagaaag
    19861 agagatataa ttttataaga gcttgtaaat tctagtaatg catagatttg taataaaatt
    19921 aagtttagga gaaatatatg aatataagta tagtagtagt aggtaaaatt aaagaaaaat
    19981 atttaaaatt aggtattgat gagtttaaga aaaggctttc taagtattgt aagttagaaa
    20041 ttatagagtt ggatgatgaa aaagctccag agaatttgag tgttaaggaa atggaaatta
    20101 taaaagacaa agaaggaaag aaaattttag ggaagataaa gcataatagt tatgttatag
    20161 ccttggctat tgatggtaag ggtttatcct ctgaagagct tgctaagact atgagtgatt
    20221 tagcagttag aggtaatagt agtctctgtt ttataatagg aggttcttta gggctttctg
    20281 atgaggtttt aggaagggct gattataggt tatccttttc taagatgact tttcctcatc
    20341 agatgatgag attgatattg ttagaacaga tttatagggc ttataggata aataatggag
    20401 aaccgtatca taaataaatg atacggtttt tgttaatttg acacttaata atattaaaat
    20461 aacaaaaaag tattgaaagt atattgcatt taaggtagaa ttaaataaaa tattataaca
    20521 ttataatgtt tatatggttg ttaataaaaa tttggaggaa aatatatgat aaaaataatg
    20581 ttggcctgtt cagcaggaat gtctacaagt ttattagtta ctaaaatgga aagtgcagct
    20641 aaagaaaatg gtatagagtc gcaaatttgg gcaataccag aatcaactat acaaaatgaa
    20701 atagaaaaat gtgatgtatt attactagga ccacaagtta gatatgtatt acctaaggct
    20761 caagaaattg ctaagccata taatatacca gtagaagtta taaatatgat gcactatgga
    20821 acagttaatg gagaagctgt tttaaataga gcgatagaac ttaacaacac taagtaatat
    20881 aaattataat gtatttttaa tatataaatt gggagaatca aaggatggat gaaaagacta
    20941 tagaaatatc ttttaatata ataggttatg caggtgaagg taaaggatta gcttttgaag
    21001 caataaaaga ggctaagaat ggaaatatag aaaaagcaaa agaattatta aaagagtcta
    21061 gggaagttat aaataaagca catagatatc aaacagaatt aatacaaaac gaagctagtg
    21121 gaaacaggac tgaaataagt gtcatactta tacatgcaca ggaccactta atgaatggca
    21181 tgaattttca acagttagct gaggaaatcg ttgatttaca tttgaaatta caagataaat
    21241 aaaaattaaa gataataaat taagagagtt cttaaaagat acattatgtg tagcacacta
    21301 attgttagat tattgattca atatttagtg agtaatacat taggattatt ttgaggactc
    21361 tttttatatt tagtttaaag ttaataaata tttattatta ataatataag attatatgtt
    21421 ataatattat aacatataat ttaagaggtg aatcatgtta agaaatcaac cattatatat
    21481 acaagttgta gatatattaa agcaaaaaat attaaataac gaatataaaa ttgatgaaaa
    21541 tattccaaca gaaagggagt tagagcaaga gtttggagtc agtaaaatta ctattagaaa
    21601 agctattgag atattagaat cagaaggata tgtagagaaa aaaagtggaa aagggacaaa
    21661 agtaataagt aatagccttt ttaataagct atcaaaagca gagtcttttt caagtatact
    21721 tttatctaaa ggtcataagt tatctaaaga gactatttca attgaaaaaa taataaatga
    21781 tacaaattca caagtattta gtatatttgg agaaaaatgt agcaagatta ctagaatgta
    21841 ttatttagat agtaagccat atatattttt tactcattat attccttttg atgaaaatct
    21901 aactgttgaa agttttaata atgaaagttc tttatatatg tacttatata ggagaaatat
    21961 aaatataact agatttgaag atgaattttt tatagaagaa tcagaactag aggtatcaaa
    22021 ggctttaaat ctagctaaag acatattatt aggaagaaaa agaaagacct atgatgagaa
    22081 tgataatata gttgagctat catttgcaag atataataca gaagtacaaa actatgtaat
    22141 taaatacgaa atataataag aatcctgagt ttattgaaat gataatatgc tcaggatttt
    22201 tgtttagttg taaaaataaa aaatattata acattataat gtttacaacc aaacattata
    22261 atgttataat atttttaata aacaagtgat attatcaatt acataaaaat tagtttatgt
    22321 tatgaaatcg ttattgcatt ataaataaag gataatatca ataatacagg gggataacta
    22381 tggaaaaatt cgcaagtttt ttagagcgca agattatgcc tgtagcagcg aagataagta
    22441 atcagagaca tatgagagct gtaagagcag gtataatagc aacattacca ctaacaatag
    22501 tggggtcatt tttcacaata ttattaaaca tacctattga tgcttatatg gattttatag
    22561 ctccatttaa agcaatatta gatataccat ttagatatac tgtaggattt ttatctctat
    22621 atgctacttt tgggatagca tcatcacttg ctaaaagcta cgatttagat acaacaggtg
    22681 ttggtatgct tgcagttatg gcatttttag tttctacaat agttccaact caagtagttg
    22741 aaccagtagg tggagttata gaagctggta gatggatgga tatggcaaaa cttaattcgc
    22801 catcattatt tggagctatc gtaacatcat taattacagt tgaaatatat cgttttatga
    22861 aggaaaagaa tattacaatt aagatgccag caggagttcc agtagaggtt tcaaattcat
    22921 ttactgcatt attaccgaca gcagtaattt taatgttatt ctgggtaata agacatattc
    22981 taggatttga tataagtgtt gcactaagcg ctatgttatc acctttaaaa ggattcttat
    23041 caggaaatag tttaggtggt gggttactga cagtattcct aataacttta ttctgggtac
    23101 ttggaataca tggtccagct ataatgggac cagtaataag acctatatgg gacatgtcta
    23161 tagcagaaaa tataactgca ttcacagaag gagtatcagc tcatgcaatg ccaaacatat
    23221 ttacagaaca atttttacag tggtatgtat ggataggggg agcaggggct actcttgcat
    23281 tagttgtaat gtttatgttt tctaagtcac aatatctaaa aagtttaggt agattaggat
    23341 ttttaccagg gttattcaac ataaatgaac ctataatatt tggagcacca atagttatga
    23401 atccaatact agggatacca tttataattg gacctataat aatgacaatt ctatcatatg
    23461 ttcttactat tacaggggtt atacctatga tggtagcaag attaccattt gcaatgccag
    23521 caccaatagc agcagtaatg agtactaact ggagtatact agcaggtatc ttagtattaa
    23581 tcaactttgt gatatcattt gctgtttatt atccattctt caaagttttt gaaaaacaac
    23641 aattacaaag agaacaagaa gaaatgagtg aaaatttata aaaatacata gatatagagg
    23701 ttttgtacta tataaaatat agaaaaagct tataagttat taagggtgaa ggtaggagga
    23761 atgacctttg cctttaattt tatatatttt aaataatgta atttgaggag gagaaagatg
    23821 gaaagaagat taggaatatc agtatatcca gagcatagta acatggaaga tgataaggca
    23881 tatataaaaa tggcttcaaa atacggattt aaaagaatat ttatgtgcat gctttcaatt
    23941 acaagaccta aggaagaagt taaaaaacat tttaaagaga ttataaccta tgcaagagaa
    24001 ttaggatttg aagtaatcct tgatatagct ccaaacatat ttgatatatt acaaatatct
    24061 tatgatgatt taacattttt tagtgagtta aatgctagtg gaataaggct tgactatgga
    24121 tatgatggaa gtaaagaagc tatgatttct ttcaatccat atgatttaaa agtggaacta
    24181 aatatgagta atgatgtagc atacttggat aatataatta cttacaagcc aaatgtaaat
    24241 aatatatatg gttgtcacaa tttttatcct caaaggggta caggcttgcc atatgatttt
    24301 tttgtaagat gttcaaatag atttaaaaag tatggaataa gaactgctgc ttttgttaat
    24361 tcaaaatacg caacaatagg gccttggaat ataaatgatg ggctgtgtac gcttgaaatg
    24421 catagaaact tagatattgc tacagcaacc aagcacttat tcgcaacagg attgattgat
    24481 gatgtaattg tagggaatgc atatgcaagt gaagatgaat taaaaagaat gtctgaagta
    24541 aataaatatc aagttgtatt taatgtagta actgaagaaa aaacttctga agtagaaaag
    24601 acaataatgg caaatgagca acactttaga agaggggata taaccaacca agtagcaagg
    24661 tctacagaag taagaaaaaa atatgctaaa gaaaatttcc ctaagcatga taatacgacc
    24721 atctttaaaa gaggagatgt agtgattggt aatgatgact ttggaaagta taaaggggaa
    24781 ttacagttga tacttcaaga ttgtgcagat gaaagaaaaa ataaggtggg tagaataaag
    24841 gaagacgaaa taatactttt agactttatt gaaccttgga gtaaatttaa acttatttta
    24901 gagggagagt aaagtgaaga tagacatatt aataaaatgt ggaaaaacag tagataaaag
    24961 tataattgat atagctatat tagacgataa aataatagaa gtaaaaaatc agatagatga
    25021 ttctaagtat caggcaaatc aaattataaa cctagatgga gaaaaattta ttagtgctgg
    25081 ttggatagat atacatacgc actgctacga aagactagac ctttataagg attatccaga
    25141 tgaggttgga ataaaaagcg gtgttactac tgtagtagat gcaggaacaa caggagcttt
    25201 agatataggt aaattttatg aagatactaa aaaatataaa acaaatgtat atgctttaat
    25261 aaatattgct aagcagggaa taacatctca agatgaactt tcaagtatga tgaatattga
    25321 tgaatatgaa cttagaagag cagttaaaaa atacaaagat tttgttgttg gaatcaaagc
    25381 tagaatgagt aaaagtgttg taatttcaaa tgatgttgaa cctttaaaag tagctaaaag
    25441 aataaaaaat gaacttaatc tacctatgat ggttcacttt gggagttctc ctccaacgat
    25501 agaggatata tttgactata tggaaaaagg agatatatta acccatatat ataatggaaa
    25561 gccaaatgga atacttagag gcaatgaggt taaaaaagaa atcatagaag ctagagaaag
    25621 aggtataatc cttgatgtag gtcatggaac cgaaagtttt agtatggata ttgcaatgaa
    25681 atctaaggat gcaggaattt ttccagacac tataagtaca gatatatata ttaaaaatag
    25741 aataaatggt ccagtgtata atctatctac aacaatggaa aagtttatat atatgggcta
    25801 ttcacttgaa gatataatag ataaagttac taaaaatgca gcagatgcaa taagtttaaa
    25861 aaataaaggt ttaataaaag aaggatatga tgctgattta actatatttg atgtggttaa
    25921 tgaagaaaaa gaacttcaag attctttgaa taagtcagta atcacaagta cgtctataaa
    25981 gccaagagca gtagtagtca atggagaata tttaaatata ggtaagtcta taggagagaa
    26041 tgagtagaag ggagaaacta ttgtatgagt agtatatttg aaaaatactc tgtaagacgt
    26101 gttataaatg cttcaggcaa aatgactata ctaggagtat caacagtaga taaggaagtt
    26161 attgaagcaa taggtgaagg gtgtaaaaat ttctttataa tagaagaact tataaataaa
    26221 acaggtgaat atatagcaaa tttattaggg tgtgaaagtg cactaatagt ttctagtgca
    26281 tctgctggga ttgcacagag tgtgggagca atgatagcaa aagatgatat gagtctagtt
    26341 tacaatgtaa ataatcctaa gaaacaagtt aaaagggaaa taataattcc aaagggacat
    26401 aatgttgatt atggagttcc agtagaagta atgataagac ttggaggagg agaagttgtt
    26461 gaggccggtt atgctaatat gtgtaacaag gaacatataa taagagagat aaatgaaaat
    26521 acagcagcaa ttatgtatat taaatctcat cattgtgttc aaaagagtat gctaagtgta
    26581 ttagatgcag tacaagttgc taaagaatat aatttacctt tgatagtaga tgcagcagca
    26641 gaagaagatt taaatgtata ttatgaattg ggagcagacc tagtgatata cagtggtgct
    26701 aaggctatag agggtcctag ctcagggtta gtaataggta agaaaaaata catagataat
    26761 gtaaagcttc aatcaaaggg tataggaaga gctatgaaaa taggaaaaga aaatattgta
    26821 ggtctaacat gtgcaattga gagatatata agtaatgata aagtaacttt aaaagaaatg
    26881 gaggataagc taaatccatt tatagacaag ataaatacta taaagggagt gagtgctagt
    26941 ataactaggg attcagcagg tagagaaata cttcgtggag aaattgactt taatgaagat
    27001 ataataaata agtcaacaca acaaattata aatgaactta gaagtggaga gatagctata
    27061 tacactagag actataaggc taatgaaggc aagattgaga ttgatataag aagtgtaact
    27121 ggaagtgatt tagatactat ttataatagg ataaaaaata tagtagaaag ttagagtgta
    27181 gagtaagtga cattataatg atttaataaa ttaaatttta ccaatggagg ggataaatat
    27241 gaattgtata ccacaatatt ataaaaatag agtttgttta aatgttcttg caggaagcat
    27301 aaaaaatgct aaagaagtat atgatgcttg tgacgggcat gtattgatag gaatactatc
    27361 aaagaattat agcactgtag aagcagctat tgaagatatg aaaaaatact caaaggaaat
    27421 agataatgca atatctgtgg ggcttggagc tggagaccca aatcagtcaa atatggtatc
    27481 tcagatttct aaagaacttc agccacaaca tataaatcaa gtgtttacag gtgtggctac
    27541 gtctagagca ttgcttggtc aaaatgatag tataataaat ggattagttt ctccaactgg
    27601 taaaccagga tttgtaaaaa tatccacagg tccattaagt tcaaaagaaa aagatggtat
    27661 agttcctata gaaacagcaa tagctatgat gaaagatatg ggaggaagct caattaagtt
    27721 ttttccaatg ggagggctta aaacaaagga tgaatatata gaagttgcaa aagcttgtgc
    27781 aaagcataat ttttatttag aaccaacagg gggtatagac ttagataact tcaaagagat
    27841 agttcaaatt gcacttgatg ctggtgtaga aaaagttata cctcatgttt atacatcaat
    27901 aattgataaa gaaacaggtg aaacgaaagt ggaagatata ggagtacttt ataaaataat
    27961 gaaagaatta ttgggataga tttatgaata tattagcttt tggtgaaata atgatgagac
    28021 tttcagtacc tgactacaag ttcttaacac aaaccaatga actaaattat ataactacag
    28081 gcacgggatt aaatatatta tctggactta aaaactttgg atacaatacc tatatgttga
    28141 caaagttgcc taataataat gtaggtaaag catcaagtgc aaatataaga aagcttggag
    28201 taaaagatga ctttatcacg tatggaggga atcatatagg agtgtatttt cttgaaaatg
    28261 ggtatggaga aagaccttct gaagtgactt atttgaatag gttaaatagt tcattttgtg
    28321 agagtaaatt acaagattat gatattgata aatgtttaga agaaatggat atagtccata
    28381 tttgtggtat tgctatgcaa cttacagatt cagttaaaga gattgcttta gaactagcta
    28441 gaaaatctta tgaaaagggg ataaaagtat tctttgattt taattttaga caatcattaa
    28501 atccagaaag gacttatgat gatttagtaa atgattataa gaaaatacta ccatattgtc
    28561 atgggctgtt tgctagttat agagatataa ataagatact taatatagat agtctaaata
    28621 taaaatctag ttcagaattg attaaaaaag agtatgatat agatattgta gctggaacta
    28681 tgagaagatt tgatgatatg aataatagat ttataaaggg atatatatca aatgatgatg
    28741 gttattttga aagtagggag tataagctag aaatatatga cagggttggt gccggagatg
    28801 gatatgtagc aggaattata tattcatatt tggaaaatat gaagagtgaa gatatagttg
    28861 agtttggggt tgctagttcg gtgttagctc atacaactta tggggataat tctttagtaa
    28921 gcacaggtca tgtacttaga ctgatgaata atgaaaaaat agatatattg agataataca
    28981 tttgtatata ataaaatttt tatctttttt agacacattt ttgacttgct tatatttgtt
    29041 aaacagagtt atttttaatg attataaata ttaaattaaa agttaaataa taaaatagta
    29101 gaaaatagca ttaactatag aaaaaatggt taatgctatt tttatcgtta atattgatta
    29161 attttatatt atagaaatga acttttctaa tgctggattt gtattttttt tattccatac
    29221 aactacaact tcaattctat tttctacttc ttttataggt actattgcaa tatcacgttc
    29281 tatcattgta tctgtaacac aaaatgaagg taaaatggat attcccatgt tagaagatac
    29341 caatgattca atagtattaa gattatttga acgttgtacc acattaggta caaaccctgc
    29401 ttcacgatat ctattctgta tcattgactt ttgtccatac tcatcccctg tttcctttac
    29461 aaaaataaat ttatcatgtt gtaattcaat taaatcgata gaatcctcat tgtaaagtgg
    29521 atgaccagga ggtaaaagag ctaccaaaga atatctttct acagttttat attcaaaatc
    29581 ttttaatggt tgattttctg gattaataac aaaagcgagg tccatagaat tattttttac
    29641 taagtttaaa agttcggtta tttcattagt acaaatatcc atagatatat tagggtatgt
    29701 attagaaaat tcttttaaat attgttgaag tttgtttttt tcatatccta ttaaggttcc
    29761 aatgctcaaa gttccaaaaa aaccatgtgc aaattgttgt gtttttgcaa tagcatcatt
    29821 gatatttttt attatagtac gtgcctcaga taaaaaaact tttccagctg gtgttagttc
    29881 aacatgacgt ttacttcttg taaatagagt tacatttatt tgttcttcta aagcttttat
    29941 ttgctgtgta acagcagttt gagaaatata aaatttttct gctgttttgg taaaacttag
    30001 attttctgct acagatataa aatattctaa ttgtttagta ttcaaataat cacccctatt
    30061 taaacttgat tgtttgtaaa ccttatttat ctataattaa agcttattat agtataagaa
    30121 aatttacttt taaagaatat tagaaaatat ttgtataaaa atatatgttt taagtatatc
    30181 tttacattag attttataaa tttattaata agaataactt atatattcca atgaaaacga
    30241 tattggattt aagtaagttt gtctcataaa atgaaagtat atcaattgat aatactttaa
    30301 ataagaaggg agaaaaaatg gaagataaat tttatgcaaa aggcaacgga aataacggat
    30361 atattaaaaa tcttgaagtt tgttccttta ataacttaga tggaacttgt ggaatgtttc
    30421 aaatggctct gtacaaaaga gatgaaaaat actatttata tggatgctgt tttggaggaa
    30481 ataaaaaaaa tggagtaatg attagcgata ttacagaccc ttataatcca caatttataa
    30541 aacattttca aatgttagac cctaaagagt atcctacaac aacaactccc aaaattcaaa
    30601 tagcagatga tttaatgata gtagcaatga gttgtggaag tggaccagga gcacttgttg
    30661 accaagctaa attagcaaat attaagtgtg aagcaggaat tagaatatat agtttaaaag
    30721 aagacccttt aaatcctaag tttttaggat attgggattg tggcttaaag catgtaatgg
    30781 gtgttcatag attcatgtac aatggtggaa gatatgtaca tttatcaagt gattgtgttg
    30841 gctttgaagg tctgatttat agggtcatag atataataaa tcctactaat ccagtggaaa
    30901 taggtaaatg gtggagacca gaccaatatg caaatggata tccaaataga acttttgatg
    30961 caggagcacc tcattgccca gaatttatgg ataaaggatg gcttcatgga cctccatttg
    31021 taagagacgg aaaagcatat tgtggttatg gaggagctgg tttagttgta ttagatgttg
    31081 aagatttaac aagaccaaga tgcttaggtg aattgccatt tatgcctgca ttttctagta
    31141 gacttgcagg tgcaagaact catacagcat taccattgcc aggaagagat ttagtcgttg
    31201 ttcaaaatga gggagaaaga ttccagttct ttaaaccaga taacattaca gatgttcaag
    31261 ctatgaataa tatacatatg gttgatgtta gtgacccaac aaaaccaaca ttaattgctc
    31321 aatttccata tcctgaagtt ccaaaagatt tcccttatcc taactttaat gttgcgggat
    31381 taggaaaacc agggccattt ggcccacata atcttcatga accaatggat aataagccat
    31441 ggttagagca aagaggagat agagtatatt gctgttattt ccatgcaggg ctaagggttt
    31501 atgatgtatc agacccatat tatatcaaag agctagcata ttttatacca ccaaatccaa
    31561 ataaaacacc agaagaatct tatttcccag gattcccagg accacgcttg gcagtaacag
    31621 aagatcttat cgttgatgat agaggctaca tcatcataga tgctttagat gatggattct
    31681 atatattaaa aatgaaagaa gattaaataa atactaaaga gggagagttg aaaatgaaaa
    31741 tactaggtat ttcttttgga acaaaaaatg gaaataatga cacaatgtgt aaagttgcac
    31801 ttaaaggtgc caaagaagca ggggctgaag tagaatttat tcaaatgtca tctattaaca
    31861 ttaaacattg tactggatgt tgtgcgtgtg ttaaaacttt attatctggg aaaggtagta
    31921 tgtgtgtatt aaaagatgat tttgaatggt tgcttgacaa aatgaaagat gcagatggta
    31981 tagtagtttc agaccctatt tttgaagaag gggcatcagg attattccat actataatgg
    32041 accgttttgg acccagagca gatagaggaa ataacattat aggaacaaaa gttgcagaag
    32101 ctataggagg aaaaattcca gattcaagaa tgcttaatga taaagtaata tcatttatgg
    32161 gaataggagg ttctgactgg ggtacaagag ttcaatgtga acatgctatg ctagctctca
    32221 taccaatgtg gaaagttata gataatgctt ggttcccatg ggcaaaggaa ttagtaatgg
    32281 atgatacaag gcttgttcaa gttcatcaaa ttggtttaaa tatagttgaa gcagcaaaag
    32341 attttgaaaa agcttcatat caaggagaag aaggtgtatg tccacattgc cacaatagat
    32401 tattctacct tgagccaggt actaaaaagg caatttgtgc tctatgtggt atagttggag
    32461 agttagatac tataagtgga aaaactatat ttagttttcc agaagagcaa ttaggtcatg
    32521 ctcatgatac tttaccaggt aagtttatac atggtgatga tattaagaaa atggaaggtc
    32581 attatgcaga agttcgtaaa acacaagaat ttaaagaaag aaaaacatct tataatttta
    32641 tagagccatt aactaaagaa aagcaagctg gagtttcatt aggataataa ttaaaatact
    32701 aagactcttt tttaaggtat gttagatgtt ataggaggct tattgtatga atgaaaagtc
    32761 aagttttaaa caaattgaaa ttattttagc agttattttt ttggtactta catattacaa
    32821 ggttagatta actccagcat ttattgttat atttatacca ctatatttaa gttattttgc
    32881 agtatctatg attgtaaatc aaatagcaat taagctattg gagataaggc aagctaggga
    32941 agctactaat tacaatatag gaaatggata gaaagaaaaa agaaatatta acactgtcta
    33001 tggggattgc tttactacct cctttatggg cagtattagc accttatata ggaataaaaa
    33061 ctggagctgt agcactaatc tgtgcaggat tatatgtaac taatggtaat aaacaaaaag
    33121 atggattaaa aataatgttt ggtttttggt gtggagattt atgggcagtg ttagccattt
    33181 taattatggg atatatgaat ttcaatcaaa acctagaatt atttttaaca cttagtatat
    33241 taggtttttt tgcagttgta attgcatcct tatttgaaaa aataattttc ctaccatcat
    33301 ggctttgtgg ttgggctata ggtctaacta ttatgacagg tgaaaatatt attaatttac
    33361 aagatatttg tattcaaatt gctgtagcta tggcagtagg agtatggtac gtgggtgcag
    33421 gtgtagattt gtttacaata tatattttga gtaaagatag gaaaaaggga aattgaaagc
    33481 atgtgaatat actttgatta taggagaatg gctatgggtt ttgaatttaa aataatgaga
    33541 agtttaatat atgtaggact tgccaaggaa gaatatagac ctaagctaat ggactggtta
    33601 tatcgtcacc atattccaga tagtattagc acttttggac catattgtac taaatatgcc
    33661 ttttatcaag catatcctac accaaatgaa ggtgagcgtt ttggtgcacg taagatgcaa
    33721 ctaacagaac attattggct tgtagatgaa catatgcctg agatggcaaa tagaattatg
    33781 acagaatata tgcctatgga tgttctacgt tggcaagggt gtataccaga tgtagaaaat
    33841 aaaagggttc atgaaaatgc agaaagtgga gatgcaggac gtgcagtagg tggagataat
    33901 ggatgtccac catttatatt tgcctttgtt ccaataaact gggaagaaga ctttagagga
    33961 aaaggacgta ctgtacaaga tggaccaaac tatcgttggc aatttatgat taagtatcca
    34021 gatggtatct ctaaagaaga aggagaaaaa tggttctatg atgaggtagt gccatacttt
    34081 acaaactgtt gctatgttaa tcgttttgtc agtagtaaaa taatgattaa ttatggagca
    34141 actgcttttg accgtgtatc agaactatgg tttgaagggg aagaagaatg gtataaagct
    34201 gtggttgaag agacaaagtc gtttattaaa aaaccagaat gggcacaaga agaggagttc
    34261 ccatatttaa aaccacaatt caatatcgca tcagtattct taggtgatat agcaactatg
    34321 gatgcatact cacagtatcg tggatatata ccaatgagat aaaaattata gtttgtgata
    34381 agtagaggag gcatttactc ttactggtag aagaagactt attgtatcct taaaagtagg
    34441 tgaatatata gttttatatt taaatttata taaaactaag actaataaat atgaaacaat
    34501 agaattagaa taatttgata atatttgtta aaagagcatt tagaatgtgt ttaaagatta
    34561 agatattcct tttatggtag cagatagttg aaataataaa actgttatca tgaaaggaat
    34621 atttttatgt ataggaaatt tttaaaaata aaagttaaat atgtttaaga acatcttaaa
    34681 gtgaaagaat cattgttaga aatatgtaga caatatagtc tttataacgt atcatattaa
    34741 caagtttaca cttggattat gaaataaaaa gagtaattcc aaaggttgta aatactatgc
    34801 tgactttgat acttcaaagt ttaggtgatt ttgtaattgt taatagaaat acgtttaatg
    34861 agcataaaga atttatctaa taataggaga atataatgta tacaattgga caagtatcta
    34921 aatttttagg aatatctaga gatacactaa aattttatga ggacaaagga ttagtaaatc
    34981 ctaagaaaaa tgatgaaaat ggatatagaa tatataatca ggttgatatc tatgatattg
    35041 caacaataaa cttttataga gaaattgata tagaaattaa aaagattcaa gaaataagaa
    35101 aaagtaagag tataaacaat ttagaactct tactagaaga gaaggaacaa atcatactaa
    35161 aagagataga atataaaaag cttctcttaa aaaaggttca gttagtcaaa gaagattgtg
    35221 acaaaataaa aacatatttt ggaaaataca caataaaaga aatgaagcct ttggaagtaa
    35281 aaggtaaaat aaaacatgtt actaactatg aggaatatga agccttaaaa gagaatacaa
    35341 acaacttaaa aaaagcagtt acattaactg atttaagaag agtaataaat tttaatgaag
    35401 aaggtataat agaagaaaaa tttataattg taagggaagt agatgactac aacaaaatca
    35461 tagaaggtga agttctatct catcccaatt gtatttatac tattattgaa gatggcagat
    35521 ggtcaactgg aggagaaaat attgataata aagtggaaga cagattaaga gaaatagcaa
    35581 tagaaaatga atgtgaatta cttggaatag tctatgttaa tatattgctt actacttatg
    35641 aagaagggat tgagcgtata tttttagaga tttatgcacc tataaaataa tataaaagca
    35701 ttgacctggg acttactcat aggtttaaac tcatgatata agcagttata acttatatag
    35761 caattaaata ttaatttgaa atgaggatta cttatgagaa cagagaaatc attaaaaagg
    35821 cctatagcca gttttattat tctaacaaat ataatttttt taccactttt tttattggta
    35881 gtagtgacta taatgttaaa acttccaaca gtgatatctg acattgcgtt gtgtatatca
    35941 gcgtggtcat ccacttttgc atttatgata ttatttaaaa aaatttatcc aggtaagaaa
    36001 tttttagtac acgtaaaaga tagatttaga aataaactta aattttcaac agtatctata
    36061 ataattagta tacaagtcct aatagctgta gtagttatat ttatattagc atctaaaaat
    36121 catggaataa tgcctaactt tacagtttca tcatggggag catttatata tttattttta
    36181 aaaaatttat ttgcaggatc attaggtgag gaattaggtt ggagaggatt tgttcaaaat
    36241 catcttcaga aaagacattc tcctttaaaa gcatccataa tagtaggttt ttggtggggg
    36301 atgtggcatt taccaatatg gtttacaaca ggattcaaag gacttgattt aataaaatat
    36361 attatatttt ttatgattgc aattatatca atcagtatta taatgacaac tttttataat
    36421 ctaaataaaa ctttgataat acctattgta atacatcaac tatttaattt tttaataggg
    36481 ataattaatg gagatttaat agagcttata aattactatg caatgttata tttaatattt
    36541 gcaattgcat taatagttat aaatccagga aatgtattat ataaaaatat tagagagtaa
    36601 tatatatctt tatggtaaga tataaagata ttattagaag aacatccaag gaggaataaa
    36661 catggtttta ctgattgttg atgcacaaaa gttaataaca aacgagaggt tatataaatt
    36721 taatgaattt gtagtaaatg tagaaaattt aattgataca gctagaaaaa ataatataga
    36781 agtaatatat gtacgtcatg atgatggagt tgaaaatgaa ttgactaaag gtaaaaatgg
    36841 ctttgaaata tacgaaaaat ttaaaccatg taatggagaa aaaatatttg ataaaaaagt
    36901 aaatagtgct tttaaagaaa ctggtctatt agaataccta acaaataatg gtgaaaaaga
    36961 tattattatt gctggtctcc aaacagatta ttgtattgat gccactataa aatgtggatt
    37021 tgaacatgga tttaatataa tagttccagc ttattcaaat acaactgttg acaatttttt
    37081 tatgtcagca gaacaatcat ataaatacta caatgaattt atatggaatg aaagatatgc
    37141 gaagtgtatt tcattagagg aaacacttag aaaaatgaaa taaaaataga gtatgaattg
    37201 tatatgtaca taagttgatt tcataaaaaa ggatgttaaa ttccaagtta aaaattagat
    37261 taaatatatt atattataga taaggcacat caattttatt ttaaattaat gtgcttttta
    37321 attgtagtaa tttatttata atcaatatga tttttattat ttaaatcaat ttttcttgag
    37381 tttctttgtc taaagaaact ttttttattt ataaaatcta cagttatatt atagtatcta
    37441 ttttagagaa gtttaatttt tgtatcaatc taaaacaaaa gttatactta aatgaaattg
    37501 aaaacgataa aattctcaaa taaaataagt attgtggagg tgatgaaaga atccaatgat
    37561 aatatgaaga ctaatcttaa taagaaagta ggggttaatg gtagtggaca aagaaattgt
    37621 atcaattctc cagattttat taaatagcac attgattaca actaatggat tacaagagga
    37681 gtctaattca tcaaagagac aaattactta tagaataaat aaaatcaatg acatgcttaa
    37741 agtaaaaaat gtaccactta tatgtttaag agctgataaa gatattatcg ttagaaaaga
    37801 aacaaaaaaa gcaattaaag aaattttaga aaaaaactat tctaagaata cttattattt
    37861 tagtaagaca gaaagacttt tatatatgta tttaatgctt tttattaaca tggatgatat
    37921 ttcaattaat cattttataa attctataaa agtaagtagg tctactgtaa atcttgattt
    37981 caaggatttg ataccagagc ttgaaaagaa gaacattaaa gttaagaaca atcgtataaa
    38041 tggatattac atagttggca atgagatgga aattaggaga gtgcttatta aaaatattat
    38101 agaaactttg tctaatgaga aaagttcaag agtatttgat atttttatta aagaatataa
    38161 tttagatagt tttgaagagg ctaaaaaaat tatattaaag ctaataaaaa aatatgaaat
    38221 tacttttgtt gaagatagat tagtagaatt tatatatata tttatctttc taaaggcaag
    38281 gatgtattca actagaatag ttactcagga aaatgtagat attcctaata tttcagttat
    38341 gagttcaatg aaggagtata aatttgctaa ggaattatta gaagtatatg ggagtgaaga
    38401 taagataaag cctcacaata taatgtatat tagtgcttgg attttaggta tatcagtagg
    38461 aaattatgaa gaagatacag aagataagga tgtaatttca aaaattgtaa ataaaatgat
    38521 gacaaagttt ggatatttaa gcggagttta ctatataagc caagaaaaaa tatttaaaca
    38581 attatattct cattttagac ctgcctacta tagactttta tttaaattac ctatttataa
    38641 tccgatttgt gaaaaagtaa aagaagaata taaattagtt tatagaattg taagtaatgc
    38701 aatgaaagag ttttgcggat tatttggaga agaaattcag gaagaagagt tagcatatct
    38761 tacaatgcac tttgcaacac ttttttcaaa taaaaaagaa tttgatggtt ttagaaaaag
    38821 aatagcatta atagtttgtt caaatggcgt tggtagttca gctattttac atgcagaact
    38881 tacaaactta tttcctgact tacactttat accaatgtcg gaatcagttg gattagaaaa
    38941 tataagtgaa cctgtagata ttgtatttac tactaattat aacgcagagg gtttaaaaac
    39001 agatgtacca gtaattaaag taagccctgt tatgaccaca aaagaaaaat ataagattac
    39061 acgtgaagtg tatattcaat taggtgatat ttttttaaga caacctaaaa tagatgaggt
    39121 tatggatata ataaaaaaat atgcaaatgt aacctctgaa agtattcttt caagtgagct
    39181 attagcttat tttacacaga tagagaattt tacatctaag gaaggagagg ggcctatgct
    39241 aagtgagatt actaatgaaa cactagtaaa aataaaggta aaagctaaga attgggagga
    39301 agctattaga cagtcagctt ctgtattagt tgaaaataac aaagtaactg aagggtatgt
    39361 agatgctatg attaactcag caaaagcatc tggaccatac atagtaatta caaaacatgt
    39421 tgcacttcca catgctagac cagaagctgg gtcaaaagag attgcaatag gcattgctac
    39481 tttagatagt cctattgagt ttgggaataa cgaaaacgac cctgtaaaat atgtattttg
    39541 tctaagtgca atagacaata atagccattt acgtgctatg tctgagctag ttgaactatt
    39601 ggaagaagat gagtttttta aggttttaga tagtgctaaa gaagctaaag aaattattga
    39661 ttttataaaa acacacgaat tataaaggag gatttaatta tgtataaaat tttaatcgca
    39721 tgtagagcag gagttggctc aagtttaatg ttaaaaataa agactcaaca aattattaag
    39781 gaaaataact ttccaattga agtagaacat ggttcattgg attcactaaa tggattcaat
    39841 ggagatgctg tagaaacatt aattgatgtt gctgaagaat taaaaaataa aaatttgaag
    39901 tttgatataa ttgggattca caatattgtt gaccgtaatg aaataaagac agcactagaa
    39961 aaattccttg cttcaaaaga gggataaatt taagatgata aggaggaata tttatggagt
    40021 ttattattag tttgctgagt acacctgctg ttttgcttgg cgtagttgca tgtataggat
    40081 tgattcttca aaagaaatca gctgttgaag tatttacagg ctcttcaaaa acacttatcg
    40141 gttttttgat atttggtatt ggtgcctctg ctatgacagg agcacttcaa aatttcaata
    40201 aactgttcca acatggcttt ggaattactg gtgttatagc ttcaccagaa gcagctacag
    40261 cacttgcaca aggtacatat ggttttgcag tttcatgtac attaattctt ggattcatat
    40321 taaatttagt atttgcacgt attacaaagt ttaagaatat tttctttaca acaggtcata
    40381 gtttgttctt ttcttgtgta ttagttttaa ttatgaaagc acatggattt gataatacaa
    40441 tcactatttt aataggtgga actatcttag ggtttatgtc tgctgcacta cctcaattct
    40501 gtcaaccatt tatgcgtgaa ttaacaggtg gcgacgaaca agctattggt cactttaaca
    40561 tgattggtta tggtttatca ggttatattg ggcgtttatt ttcaaaacat aaagatgata
    40621 caacagagac tatagaattt cctaagtggt tatcaatatt cagagacttc ttaatgggtc
    40681 tatccatagt tatgttaatc ttattttata ttgcaacatt aaaagctggt caagcattta
    40741 ctcaagaaat tgctggttca acacattggt tggtttttcc gcttatagag gcatttacat
    40801 ttgtggcagg tatgtcaata ttgatgtcag gagttcgtat gttcttagct gaaataactg
    40861 cagcatttgt aatcatttca gaaaagtata ttccaggttc acgtcctgcg ctagatgttc
    40921 caaccgtatt tccatatgca ccaaatgcag ttattattgg gtttatatca gcatatgctg
    40981 ctggtctatt agcaattgct attatggcat catttcctag tatatttcct gtagtaatta
    41041 ttccagctgc tcacatatgt ttcttctcag gaggtacagc tgcaatattt ggcaatactt
    41101 caggaggatg gagaggggct attgctgggt catttgttgt ggggttatta cttgcatttt
    41161 taccagttat actatatcca gtttatggga ctcttggtat agaaggagca actttcccta
    41221 atattgatta caacgtagtt ggttcgatat tacataatat tttacaattt ataaaaccta
    41281 tttagactaa attatataaa agtatagtca tagttactat gatttcaagg aggatacatc
    41341 ttatggaaaa tacattatct gaatttttag ataatatatc taatgaattg tcagaattta
    41401 ttgcctcaat agatgagaca tctataaata aagcatgcga attaattctt gaagctgaaa
    41461 aaaatcacgg acgtgtacat gtgactggta ttggcaaacc aggacatgta tcgggatata
    41521 tttcatcatt gctttcatct actggaacgt cagcatatat attgcatggt acagaagcag
    41581 tacatgggtc aagtggtcaa gtagttgaag gagatgttgt tattgcaata tcaaatagtg
    41641 gtgaaactca agagttaaaa gcaactctta agactctaaa agtaaatgga gcaaagataa
    41701 ttggtgtaag tggtaatgag agttcatttt taaaaaatat ttctgatata ttcctgtttg
    41761 caggtgttaa gcaggaagga gattgtctca ataaagctcc tcgtgcttca attttagctg
    41821 aaacaataat attacaatca ttaagtgttg tattacaata tgctaaaggt cttaatacac
    41881 aggagtattt gaagtggcat ccagcaggaa gtcttggaaa gtctattaga gagggaatat
    41941 aattttatga aatataaagg gattatatgt gcaatgataa caccatttga tgaaaatcaa
    42001 aatattaatt cacaagcaac atgtcaatta attgattatt tgattgaaaa gggaatttat
    42061 ggattattta ttttgggcac taatggtgaa tgtcatgtat taacagatga tgaaaaagtt
    42121 gaatttgcaa agattgttat taatcataca aataatcgtg tacctgtttt tgttggaaca
    42181 gggggtaatt caactcgtga agttgtagat ttatctaaga gaatggaagc gattggagca
    42241 agtgcattat ctattattac accatacttt gttacaccaa cacaacagga attaatctta
    42301 cattacaaaa ctattgctaa ttcagttaat ttacctatta taatgtataa tatgccaggt
    42361 aagactggaa ttaatattga accagagtca gtacgtgaat tatcaaagat aaagaatatt
    42421 gttggaatta aggatagtag tggtaaactt gacaacatga aagcttatat tgaagcaacg
    42481 aagaatgaga attttagtgt gttttcaggc tcagattcac ttattctgga tagtctaaaa
    42541 gctggtagcc aaggagctgt tgcagcaaca acaaacttcc tcacagagat tggtatctct
    42601 atctatgaaa actttatgaa aggggatttg gaagaagctc aaaaatctca aagtagtata
    42661 gaagaacttc gacgtatact gaaacttgga acaattccat ctgttatgaa gaaaactatt
    42721 gtcttgaatg gaattaatgt tggaactgca cgacttcctg taactgaacc aactggggaa
    42781 gtacttgaag aaattaaacg agtagttgaa aattatagga caatacttaa taaaagtagt
    42841 gagaatagat aatataaata gggaaattac actggtatat aattaatata tatattataa
    42901 aacatgacta aaattaatga ataagtaaag tggtttgatg agtatagagg tgataatatg
    42961 aaaaagatga atataaccaa aagaatgtca gaatgtgggg ctcttgcaat tgtaagagaa
    43021 gaaaatctta accgtgcatg tgaaattgct gaaggatgta taaaaggtgg tataacagtt
    43081 atagaaatga gttataccct aaataatgct ggagaaataa ttcaagggct taataaaaag
    43141 tatggtgaaa tactttgtgt gggagcagga acagttttgg atagtgaaac agctcgtcat
    43201 gctatattac atggagccca atttattatt gcaccaaatt ataatgaagg tgtagcgaag
    43261 atttgcaata gatatcagat tccttatgct ccaggatgta catcattgac tgaggcagta
    43321 gacgcactaa gtttaggagc aacttttata aaagctttcc caatatcaga tttctatgga
    43381 cctaaactag taaaggtatt taagacacca atacctgata tgccagtttt agcaagtgga
    43441 gggcttaata ttgaaaacct acatatatgg ttagaaaatg gagttgatgt atgtggattt
    43501 ggtggtttat taactaaagg ttctgttgaa gatattgcta aaaatgctaa aaagattcgt
    43561 gaaatcatta aaaatgtaag aaaaatttaa catagggctg tatcatagta tcttataatt
    43621 actatgatac agccttttta atattattca aatttaaaac agtttacatt tctattatat
    43681 tacatataat ttcagtaatt ttatcttaat ttttctatct ggaaaaaaat gatttaaaac
    43741 ttaagttatg atataatttc attaaaagaa ggcgattttt agtaaatatg agaaaataat
    43801 gtgtttttag gggaaaagtt aaatacagat ttaagtgaac aagaatcgat aattaagata
    43861 ttaaatggtg aatagggcat caaaaaaatt actaaattaa aagttttaga gggagaatct
    43921 tagcaagtgt gtgtgaaaat tcaatttcaa acaaaaataa ttgtaaatga aatcaattta
    43981 tacacgttga agattcactt ttatcattat attaaaaagc agagtgaatt aaaagtgagt
    44041 ctaaagatag gaggataatt aaaacaatga aagaagacat taggttacat cgaatgtgta
    44101 aaaaagaaca agcaacaaag aatgaagtta tatcagagga tattctaaaa tctatgagta
    44161 ttggtgtcag taaacatttt ttagatgatt catttacaat aatatgggga aatacatcat
    44221 tctatcaatt attaggatat acagaagaag aatttttagt taaattttct agtttaaaag
    44281 attattatga acagaatctt tatcattttg aatctatgaa aaatcatttt ttagaggctt
    44341 atcatcatgg cgaaaagagt atagaatatc aaatttgcat ttcagcaaat aatggagacc
    44401 ctgtttgggt aataatgact ggtatattaa ttgaatcaca agataatgag aaatcaatgg
    44461 tctatatatt gtattcaaaa attaatgagt taaaaataaa gcaagaagaa ctatctgata
    44521 tggaaaaaga gagtacagag aattttaaat ggatgatgtc agagtatgct gggaatgttt
    44581 atattagcga tatggataca tatgaattac tatacttaaa taagcactca tgcaatactt
    44641 tacaggcatc agcaaatgaa cttattggaa gaaaatgtta tgaggtaatt caaggtagaa
    44701 cttcgccatg tccgttttgt acaaattcgt atttaaaaaa agatgacata tatgaatggg
    44761 aattttataa taaaaaactg aaacgaacat ttataattaa agaccgtatg ttgaattggc
    44821 aagggcgaag agttcgtatt gaattatctt atgatatgta tagtgctgaa tataaattag
    44881 caaaaaaaga tcaggaaaga gaatcaattt tgaaaactat tccagcagga atggttagat
    44941 tagatgcaag agattatctt actatacttt ggtgtaatga tatatttttg aatatgattg
    45001 aatatacaaa ggaacagttt gcagaggaac ttaataatca atgtggatat ttattttctg
    45061 aagattataa acgtagtaag aaactggcac aagatttgaa ggagtctggg gataatgttg
    45121 tatttgaagc aaaaatatat acacgttcaa gggaagaacg tatatggact gttacattgt
    45181 gttatatcag tggggaagac agttgggatg gaataccatc tttttatagt ataggtattg
    45241 acataacaaa ggaaagaaaa cagattgaga aattacagca tatatcagag aaagatgcat
    45301 taacaggaat ttataatcgt gctgagactg aaaggcagat taagaaatat tttgaaaaaa
    45361 atttaaatgt tatgggtgca ctgtttatga ttgatacaga taattttaaa cagattaatg
    45421 atacagaagg gcatatgatt ggagatatag tacttactga gatggcatca ggtatgaaaa
    45481 aaataatgcg tgacagtgat gttgttggaa gaataggtgg agatgagttt acaattttta
    45541 tgaagaatat ttcctcagtt aaagatgcag aaaagaaagc agaagaacta ttgtatatgt
    45601 tccgtcatct atttcagaaa gaaaaaagtt ttctaaaagt aacttgtagc ataggaatag
    45661 ctatatatcc aaaggatggt actacattca aggaaatata tgcaagagct gataaagccc
    45721 tgtatcaagc aaaaaatatg ggaaaaaata attatgtaat ctacaaccaa gattatttta
    45781 aagaattaga agaatttgat tgttcttctc ttggtacagt tattgattca gaaaaacaat
    45841 acgctgaata tcctgataat cttacacgtt atatattccg tatgctttat cagactgatg
    45901 atatagacca ggcgattaat atgatacttg aggttgttgg gaaacagttt gatgtaagtc
    45961 gtgtttatat ttttgaaaat acagaagatg gtagatatac ttctaatact tatgaatggt
    46021 gtaatgaagg aatctcttct caaatggctt atttgcaaaa cagcaattat caagactatg
    46081 aggattatga aaagcttttt gaggatgaat tagtttttta ttgtcgagat atccatacat
    46141 taagtcctaa actagaagaa ttgttttcta gtcaaggtat ccactctaca cttcaatgtg
    46201 catataaaga agatagagtt ttcagtggat ttgttggatt tgatgaatgc acaggactac
    46261 gtctatggtc acaagaggaa ataagtacat tatcattgat ttctcaaatt atatcaatgt
    46321 ttttacaacg taaaaaaaat aataaactta atcaacagat tcaacaatac cagaatattt
    46381 tagataatat tgaccagtgt atttgtgtga ttaataaaga aaataatttt ttattatatg
    46441 taaataaaaa atttaaaaat acatattcta aattccaaat tggacaacct tattatcttg
    46501 attctataga tattcataag atatccatta tatggaataa aaaagaagca tacctatgta
    46561 ctagcacgat ttaatggtaa agtaaagttt atattggact taaattaaat ttcgttttaa
    46621 ataagatatt attttagttg cttactgaaa agactagaat ctatattaat gaattatact
    46681 aattttatac cactattaaa atattgtttg ctaaaaataa aaaatagcct taaaacagtt
    46741 gattataaaa aattaactgc tttaaggcta tttttatctt tgtgacgaaa tagacacttg
    46801 ccatttagaa tgtgacaata tagtagttgt ttagaaaagg cacaaagcat tatactttaa
    46861 ctaaagtaaa atgtattttg cttttagaat aaaaacaatt aggagggagc tataattgaa
    46921 aaagataata tatgtattat ttacaattga actattaata gtatctttat taggtctaaa
    46981 tatagttaaa gataatgaaa ttaataatat tttatataac gatacaacaa gtatatctgt
    47041 aatgtttaaa gattataaaa agttaaataa aaattatagt aagtggatta aagatattgc
    47101 agatgaaaat aatgtggcta tatcaaagta tgtttttaat aataatgaaa aattatctat
    47161 atatacgact gatacaagtt tgaataacaa tataaagtta aaatcaggaa aatttcctaa
    47221 tactaattca aatgaattta ttagtaatat aaacactaat aatagtaatc aagtaggtaa
    47281 attctcaact atgagtaaag acatatcaat tttaattagg gattttggta aaataagtga
    47341 agttggagaa tcaggaatct tatatatttc aactcaaaat gaagatacaa taaataaaat
    47401 attaagagaa ctaaatgtag acagaagtat ttttgtagca agattacatg atagatatgt
    47461 taactcaaat ttatacttaa atccttcaat aattagagat ttaatactaa ttatcctttg
    47521 tttcttggct acaataatac attattcaat tagtgtgagt agagaggctt ctattttaag
    47581 attaaatgga tactctaagc tagatataat tgctagagtt ataaaaaata tacttagaat
    47641 tatgatttta tcatctgtca cagcattatt aatatatata atctatattt taaaattaaa
    47701 tataggtgtt agagcatgct tatactttgc tatgttttcg attatatgta ttttgataaa
    47761 tattttgata tctatattaa tagttggttt taatagtaga agttctaaat atgtgctatc
    47821 tctaaatgga aaaaaatctt atggttttgt aaatataatg catttcacat tgaaaattgt
    47881 atttgtatca tttcttatat tatccataaa taactgtatt ttaaattata atatgctaca
    47941 aactcagtta ggaaatctat ctgagtggga taaagcaaaa aatgtttata atcttactct
    48001 taaagataca ggcgaacaat ctttaggaaa ggaagaagta attaaaaatg ccaagattaa
    48061 aggtttctat aaaaatttgg tagaagaaaa agatgctttc ttaatagatt caactaattt
    48121 tgaaaaactt gaagatggaa gttacatgta tgaaataaat tcaaagggta aaaacccaga
    48181 agtaagtcaa tatggtaaaa atataaaaat aaataaaaac tatcttaaag ttaaccctat
    48241 ttactcaaat ggcaaagaaa tatttaatct aatcgattat gacgataaga ctataaattt
    48301 attagttcct aaaaagttac aagtacatga aaatgagatt aaaaaagagt ttagagaatt
    48361 attttatttt gaaaaaatag aagtagaaaa tatgtacaat aaagagttaa atagagagtt
    48421 aaataaaaca aagaagtccg atttaaatgt taatataata tatgtagata ataatcaaag
    48481 ctattttact tataactccc ttgttatgga tgacaatagg aatctcattg atgacccaat
    48541 agcaatagtt gacatagata atattgatga ttctttttat ctttcttata tatctagatg
    48601 tgtatatttt aattctaaga aattagatgc ccttgcagat ataagtagca ttattgaagc
    48661 tcaaggtgta gaagcgcata tccaaagttt acatgctgtt tataatgagt atggtttaga
    48721 aataaataaa ctagaaaaat atttgaatag tgaaattttt actataataa taatagcaat
    48781 atcaaactta atgattacct acaatatagt agctagctac tatgaaagaa ataaatataa
    48841 gttatatatt aaaaaaatat ttggttactc aactacagct agaagtatgt tgttaatagt
    48901 ctcattaata ttaacaaaca ttattccaat aggcattata tcaacaagag tagatttaag
    48961 taataatatt atcttatttg gacttctaat attagttata gaagtaattg tatctattgt
    49021 attagacaag attatctcaa atagttcatt taataaaatt ataaaaggag aacattaaaa
    49081 agatgatttc attaataaat atatctaaaa aatttaaaga taaatctata atagacaaat
    49141 ttaacctaga aatttctaag ggcgagtttg tagcgataac aggaaatagt ggttcaggta
    49201 agacaacttt acttaatata atgggattat tagaaaaacc taattctggt gatattataa
    49261 taaataatat aaaaaatcca aatagtaaac agataagaat tttacaaagg gatttttatg
    49321 gctatctatt tcaaaattat gctttaattg agaatgaaaa tgttgaaaat aacttgaaaa
    49381 ttgctttaaa gtatcaaaaa aatatcaatc ataatgaaaa aataaattac gcacttgagt
    49441 ctgtaggtct tagaaaatac aataaaaaaa agatatacga attgagtgga ggagaacaac
    49501 aaagggtagc attagcaaga gtaatattaa aagaatgtga agtaatattt gcagatgaac
    49561 ctacaggtaa tttagataaa aaaaatagag acatagtatt tgatatactt aaaagtctaa
    49621 ataagaatgg taaaactatc gtatttgtaa ctcatgattt agatttatcg gagcaggcag
    49681 atagagttat aaaattgtaa taatataaac tataataaat ataagaaaga gattacagct
    49741 aaattaagtt taataataat acgaatagaa tttaaacatt aagtaaatta gtagtttgta
    49801 aatataagta taatatgata tcataaaggc tcaaaagaaa ttttatataa taagaaatct
    49861 aaaaataaca aatggaggac atataaagat ggaaaattta ccaaattgcc cgaaatgtaa
    49921 ttcagaatat acttatgaag atggaactct tttagtttgt ccagaatgct ctcatgagtg
    49981 ggcattagga ttgggagctg aagaagattc aaatattatc aaagatgtaa atggaaatgt
    50041 attaaatgat ggagattctg ttacagtaat tagagacctt aaagtaaaag gaagttcatc
    50101 atctataaag attggaacaa aagtgaaaaa tatccgttta atccatgact catcagatgg
    50161 acatgatata gaatgtaaga tagatggttt tggagctatg aagctaaagt cttctgttgt
    50221 taaaaaagcg taatattata aataagctgt agtgtttatt gtttttcagt acagtataaa
    50281 accataagaa tctattaagt aataaaagtt tgtttattta tctttatatt ttcttatggt
    50341 tttattaaat ataaatttta ttgaacacca aaatattctc tcctctacaa ataagccaat
    50401 cttatttaaa ctaatatact ttgtactatt tatactgaaa ttgtaaattt aaatgctatg
    50461 ttatttaggt ttgtatagtt aatttatata tttctgattt tataaattgt atttattatt
    50521 atatcttgat tctaaaatca ttaaaaattt caacttctat aaagatttac aatttatcaa
    50581 atacattatg agtatataaa caatttatta ttacagtgct acatttaaaa taagtcttag
    50641 tataaaggta ttgttacaag tataaagaaa cttatgtatg ctattggaat aaataatacc
    50701 ttaaattata attaagaata agaactgtta aataagggac ttgattatca tttagttata
    50761 gataatataa tattaaaaat aaagtttaag aggagaaaaa atatgataga tatacataaa
    50821 aatatatatg ataataagtt atttgaggag ctaaagattg actgtaagaa gtgctttgga
    50881 ctttgctgtg tagctcttta tttttctgct tcagatggat ttcctatcaa taaggaatca
    50941 ggaaaaccct gtattaattt acaaccagat tttaaatgtt ctgtacataa tagtcttatt
    51001 aagcgaggtt ttaaagggtg tactgcatat gattgttttg gttcaggtca aaaagtagca
    51061 caagtaacat ataaggggat agactggatg caatcaccag aactaacaaa tcaaatgtct
    51121 gaagtgtttt taattatgag acaattacat gaaatgttat ggtatcttaa agaagcatct
    51181 gtattaaata tatcagatac aatcaaatca aaaattgatt taataattga agaaactgaa
    51241 aagataacta atatgggccc agaacagata attaatttgg atatcatatc acatagaaca
    51301 aaggttaatt tattattatc acaagcgagt gagtctatga tgggaaaggt taaatcattt
    51361 atcaagactt ctactcttaa aaacatgaaa aaattaagta aaaatataga tttaatagga
    51421 gcagacctta gaaaaataaa tctaatagga gcagatttaa gaggtagatt tctaatagca
    51481 gcaaatttaa gaaatacaga tttgagtggg gcaaatttaa taggagcaga cttaagagat
    51541 tgtgatatta gaggtgctaa tcttgaaaat agtatatttt taacacaatt acaagtaaat
    51601 actgcaaaag gagattctag tacgaagtta ccagcttcac taatacgccc taaatactgg
    51661 gaaaaataat taaattaata cataatttta caaaatatga acttttttga ctttaattca
    51721 tattatacat tagagattct ttacaagaat ttcttaatat aatataggga gagatataaa
    51781 aatgagttat caaaattata aatgtgaaag agattatgtg tatgatgatt ataaatacaa
    51841 taagtgcaac aagtgctata atgattatga ggaaatgaca catgttcatg aatattcaga
    51901 aagtgttaag ttagcagaag aatgtgagga tagacataac caccgtgcag caggggttac
    51961 tggtgaagtt ataccaataa atggaggtac aaatcatgtt cataaaataa atgataatgt
    52021 agatttcctt gaccatttcc ataagatatg tgttacaaca gggccagcaa ttagaatacc
    52081 tggaactgat aaacacattc acttaatatg tggagaaact actgttaatg atggtcattg
    52141 tcataaattc ctttttacta ctcaaataga agcaccttta gtataagctg atgtgaatta
    52201 ttgtagtgaa tttgctagat tatagtataa tatataatag ataatattta aacttaatga
    52261 ggactatgat atttaacctt cgtagtcctt tctaagtttt gtataaaaaa gtgttatgca
    52321 attaaaagtg atatagggag gaaattttta tgaaatctgg aataattctt gaaatgaaac
    52381 aaataaattc aacagaatat gacgaaatgt tgcaagtctg ggaatcatca gtaagagcaa
    52441 cgcatgattt tttaacagaa aaagatattg aatctttgat accattagtt gagattggac
    52501 ttaaagaagt agaaaatata gtttgtataa aagataatga tataataaag ggctttatag
    52561 gaatagataa agataaaatt gagatgctct ttatagaaga taaatataga ggaaatggta
    52621 ttggtaaaaa attaataaaa tatgctatag ataaatacaa tgtaaaatat gttgatgtaa
    52681 atgagcagaa taaaaaagct gtaggatttt atattcactt aggatttaaa gtgtttgata
    52741 ggtctgaaat agatggacaa gggaacccat ttccaatact acatatgaaa ttaatttaaa
    52801 gttatacttc ccacttattc gcaaaaatat ttacttaaat ttttatacaa ataaaagtcg
    52861 tgacagtctt aggacacgac ttttttacta cactattaaa ttgaaattta tctgttctat
    52921 attatgatta ttagatgtag tttttttatt ttaactatgt acttaaatac caagtatacc
    52981 catacctatc aagtacttca gcagcgccat cattttgatg tggtggtggg tgtggtaaaa
    53041 attcttgtac aagtgtacca ccttcggcaa gttttacaga tatttcttct atcgcttctc
    53101 gagatggtag aacaacacaa atcgccatgc cagaaattac aacaggcttg tctgtatccg
    53161 cacccattat acaaccttcg tattttgtga aagtaacact tgcgtgcata ataggattgt
    53221 ttggattgct gtctaatcgc gcaaatatag tttttcctcc tccgaaagcc tcagcataaa
    53281 aattaaatgc tttttcacaa gtaccattaa atattaaata tggagttagc atgaccaata
    53341 tatcctcctt gatatagaat aatttatagt tgcacctgat aatctaaatt atgatttagt
    53401 aaattgtatc atagggaaac cttgaagtat ataatttttt attattatat tatacaaatt
    53461 tattaattta aaaaaatgtt atatgtacgg aaggtttaca ggataaaata taataaaaag
    53521 attaaggtga cattataaaa aatgtatatt gacattgacg ctacgtaatt gtatatactt
    53581 tgtcttaaag gagatggtaa atatgaatat tatagaaatt cgctcagata aaatatacaa
    53641 gaagataatg gatgcaccaa taaacaaaaa agaagatata tacagatatg aattaatgaa
    53701 gccttttgaa tttaagtgga agtgtatgaa tgttccaata gttgctagac agaaaggtgg
    53761 atatgatgta attatagcaa gtgaaatgtt aggggtttta tcgcctaagg atattgatga
    53821 aaagcaaaaa aagaatataa atgtgttatc tgctgataaa atttggggca cttgcaaaga
    53881 aaccatagaa aactctataa atgcttttat aaaagaaggg tatgatttaa acattaagga
    53941 ctataaatat tcaatattat tggcgaatcc aaatagtcct tatacaatat taagtgatgg
    54001 atactggggt gatggtggga ttcctggata tatatttcta tcattggttc ctaatgaata
    54061 tactatcaat agattaccag tattaatagc acatgaatgt aatcacaata ttagatttca
    54121 gtttatagag tggaataata atataacatt agaagaaatg atgataaatg aaggtcttgc
    54181 agaaaatttt gcaacatgga tgtttggaga ggaaatgtta ggaccttggg tcagtagaac
    54241 agatatcgaa acattaaata cttatataaa gccaataata aaaagtgctt taaaagaaac
    54301 tggatttcaa aatataacat cttatcttta tggtgatgat atagctaaaa tgcaaggata
    54361 ttttccagta gggttgcctt attgtgcagg atatgcttgt ggatattata tgattaagta
    54421 ttatttagaa aagacaaata aatcaataat cgaagcgact ttattgcctt atagtgagat
    54481 aatcgaagca gtaaaagagt tttgggaata aattttataa attgcataat ttgaaataaa
    54541 ttatgcaatt attttatttt taagaaaaaa taaattgaca ataatttata agaaatatac
    54601 aataatatat ataaagaaat atttcaatat ttaaaagttt taagatttca tatctagagt
    54661 agctaacatt acatgttagt ttcttgttac ttatctaatt actttatatt tcgtagttat
    54721 atataaaatt actataaaaa atgaaatata ttaaacaatc aactgaaaaa tgaaatgtta
    54781 ataaaaaaaa attcaaatta taaatattaa atgaatataa ttaaaacaaa tatataaatt
    54841 taagtataac actcacattt taatgtattg tgaaattttt gttgaacttt atgtaccata
    54901 acttataaaa tttatgtcat gttatatttg aataagtaat aaaattgtag atgtaaatta
    54961 atctacaaaa tttatatgaa atggagttga tgctatgaca gaattaatga ttataagtgg
    55021 gttagtacta ttgatttgta taacttctag caaggtattg tataaatttg gagtaccaat
    55081 attattgatt tttatcttac tagggatgtt atttgggtct gatgggatgg taggaatcta
    55141 ttttgataac tatgaactga caaaacaatt atgttctatt ggactaatat ttataatgtt
    55201 ttttggaggg tttggaacaa actggaatat ggctaaacct gttgctatac cttctatatt
    55261 aatgtcttca ctgggtgtaa taattacggc tggggttaca ggtatgtttt gccacatggt
    55321 aataggaact agtttacttg aaggtttatt aattggggca attgcagctt caacagatgc
    55381 tgcatctgtg tttgctgtgt tgcgttctca aaaactaaac ttaaagggtt cacttgcatc
    55441 attattagaa gttgaaagtg gaagtaatga cccaatagcg tatatgctga cactaattct
    55501 tttgacattg atgaataatt ctggggtaga acttattgca ccaatggtaa taaaacaaat
    55561 agcttttggg ttagctatag gatttatact agcaaaatta tctgtttata ttttaagaca
    55621 ttcaaacttc gagatagaag gattttatac tatatttata acagctattg caatactttc
    55681 ttatgcattt agcgagtatc ttggaggaaa tggatattta agtgtttaca tttcaggtat
    55741 tattatagga aatagtagga tacctcttaa aaaaagtttg gttcatttct ttgatggaat
    55801 ttcatggatt atgcaaataa tgttattctt tatgttagga ttgttatctt tcccttctaa
    55861 gttaccaaat gtatttggta tagcaatggc catatctata ttcatgatag ttattgcaag
    55921 accattagca acatttatag ttctagcaaa atttaaattc tcaaataaag aaaaattatt
    55981 catatcgtgg gtaggtctta gaggtgcggc ttcagtagtt tttgcaattt atgctgttac
    56041 tcaaggagta gccatagaga atgacatatt ccatataata ttttttatag cgttgctttc
    56101 agtaggaata caaggttcat tgataccaac tgtagccaga aagttggatt tagttgatga
    56161 taatacacct gtattaaaaa catttaatga ttatgatgga gaaattagct ctaaacttat
    56221 cgaagtaaat gttgatgagg gtaataaatg ggctaataag agtataatag attctaatat
    56281 accagacgat atattaatag ttatgataaa aagaaaggaa caggttttaa tacctaaagg
    56341 ttcaaccatt ataaaaacag gggatatttt ggtattgagt ggagatagaa ttgaagaatt
    56401 aatacaatta taatttattt tgtattgact ttaacctaag gggaaagttt aaacttaaat
    56461 taatcaaatc taatgcgcag gtgatgagat gttttctata ggaatgtttt ctaaaataaa
    56521 taaaataact acaaagacat taaggtatta tgaagatata gggttgttaa aaccagaata
    56581 tgtagatgaa tttacaaaat ataggtatta tacaacagag caattaccta aattacatca
    56641 gataataact ttaaaacaaa tgggtttatc attagctgag ataaaaaaag ctgtagaaaa
    56701 tcctgaagaa gttgaaaata ttttaatgaa taaagaaaaa gaaatgttgg atgtaattaa
    56761 aagtgaagaa tataaaataa ttaaactacg taattacctt atgaatttga aaggagaagt
    56821 caatatgaat gatgtaatta ttaagtcttt accaaaagtt aaagttgctt ctatgagaaa
    56881 agtgttaaag gggtatgatg aactttttaa tctatttcca aatgtaatgg ctgaagaaat
    56941 gatgaaaaca gggtgtacat gtgcagttcc tgattattgt tttaatatat atcatgatgg
    57001 tgaatataga gagactgata tggatgttga aatgtgtcaa gctataaatg atttaaagga
    57061 agatacggat atattaaaat ttaaagaatt agatgcagtt gaaaatgcag tttgtatatt
    57121 acacaatggt ccatattcaa cattaggaaa aacttacgca gatgcattta aatggataga
    57181 ggataataaa tataaagtgg ttggaaatcc tagagaatct tatatagatg gtatatggaa
    57241 taaagagagt gaagaagact ggttgacaga agttcagata ccagtgaaac gcatgttata
    57301 atatgtctac aaagtataaa taagaataaa aaaggtggtg tctcaaagta aacattttag
    57361 tttatgagac actctttttc atatgaaatc aagtatattt ttataattac gattatgaaa
    57421 aaaggctta
//
LOCUS       DAGSWI010000006        54381 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.006, whole genome shotgun sequence.
ACCESSION   DAGSWI010000006 DAGSWI010000000
VERSION     DAGSWI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.006"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            335..1174
                     /locus_tag="MJF80_000314"
     CDS             335..1174
                     /locus_tag="MJF80_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBZ0278996.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1171..2352
                     /locus_tag="MJF80_000315"
     CDS             1171..2352
                     /locus_tag="MJF80_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0278997.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2656..3558
                     /gene="ptb"
                     /locus_tag="MJF80_000316"
     CDS             2656..3558
                     /gene="ptb"
                     /locus_tag="MJF80_000316"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBZ0278998.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3580..4659
                     /gene="buk"
                     /locus_tag="MJF80_000317"
     CDS             3580..4659
                     /gene="buk"
                     /locus_tag="MJF80_000317"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBZ0278999.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4649..5326
                     /locus_tag="MJF80_000318"
     CDS             4649..5326
                     /locus_tag="MJF80_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0279000.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            5369..5584
                     /locus_tag="MJF80_000319"
     CDS             5369..5584
                     /locus_tag="MJF80_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBZ0279001.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5613..6692
                     /locus_tag="MJF80_000320"
     CDS             5613..6692
                     /locus_tag="MJF80_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBZ0279002.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6692..7444
                     /locus_tag="MJF80_000321"
     CDS             6692..7444
                     /locus_tag="MJF80_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBZ0279003.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7445..8002
                     /locus_tag="MJF80_000322"
     CDS             7445..8002
                     /locus_tag="MJF80_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBZ0279004.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8172..9518
                     /locus_tag="MJF80_000323"
     CDS             8172..9518
                     /locus_tag="MJF80_000323"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBZ0279005.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSLPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATDLANLIQEKLS"
     gene            9785..11617
                     /gene="glmS"
                     /locus_tag="MJF80_000324"
     CDS             9785..11617
                     /gene="glmS"
                     /locus_tag="MJF80_000324"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBZ0279006.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            12069..13364
                     /locus_tag="MJF80_000325"
     CDS             12069..13364
                     /locus_tag="MJF80_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBZ0279007.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYLDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            13855..14592
                     /locus_tag="MJF80_000326"
     CDS             13855..14592
                     /locus_tag="MJF80_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBZ0279008.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            14613..15866
                     /gene="murA"
                     /locus_tag="MJF80_000327"
     CDS             14613..15866
                     /gene="murA"
                     /locus_tag="MJF80_000327"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBZ0279009.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            15953..17017
                     /gene="spoIID"
                     /locus_tag="MJF80_000328"
     CDS             15953..17017
                     /gene="spoIID"
                     /locus_tag="MJF80_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBZ0279010.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            17083..17751
                     /locus_tag="MJF80_000329"
     CDS             17083..17751
                     /locus_tag="MJF80_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBZ0279011.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            17849..18109
                     /gene="spoIIID"
                     /locus_tag="MJF80_000330"
     CDS             17849..18109
                     /gene="spoIIID"
                     /locus_tag="MJF80_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBZ0279012.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            18306..19325
                     /locus_tag="MJF80_000331"
     CDS             18306..19325
                     /locus_tag="MJF80_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBZ0279013.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            19341..19769
                     /gene="fabZ"
                     /locus_tag="MJF80_000332"
     CDS             19341..19769
                     /gene="fabZ"
                     /locus_tag="MJF80_000332"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBZ0279014.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(19880..20389)
                     /gene="yyaC"
                     /locus_tag="MJF80_000333"
     CDS             complement(19880..20389)
                     /gene="yyaC"
                     /locus_tag="MJF80_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBZ0279015.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      20578..20681
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            20818..22011
                     /locus_tag="MJF80_000334"
     CDS             20818..22011
                     /locus_tag="MJF80_000334"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBZ0279016.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            22327..23424
                     /locus_tag="MJF80_000335"
     CDS             22327..23424
                     /locus_tag="MJF80_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279017.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPVIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            23688..25907
                     /locus_tag="MJF80_000336"
     CDS             23688..25907
                     /locus_tag="MJF80_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBZ0279018.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEILKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            25910..26692
                     /locus_tag="MJF80_000337"
     CDS             25910..26692
                     /locus_tag="MJF80_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBZ0279019.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            27024..28937
                     /locus_tag="MJF80_000338"
     CDS             27024..28937
                     /locus_tag="MJF80_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBZ0279020.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIQGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            29078..29386
                     /locus_tag="MJF80_000339"
     CDS             29078..29386
                     /locus_tag="MJF80_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBZ0279021.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            29410..29736
                     /locus_tag="MJF80_000340"
     CDS             29410..29736
                     /locus_tag="MJF80_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0279022.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            29775..31109
                     /gene="celB"
                     /locus_tag="MJF80_000341"
     CDS             29775..31109
                     /gene="celB"
                     /locus_tag="MJF80_000341"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBZ0279023.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            31120..31638
                     /locus_tag="MJF80_000342"
     CDS             31120..31638
                     /locus_tag="MJF80_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBZ0279024.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLTDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            31734..32693
                     /locus_tag="MJF80_000343"
     CDS             31734..32693
                     /locus_tag="MJF80_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBZ0279025.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCVATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            32742..33794
                     /locus_tag="MJF80_000344"
     CDS             32742..33794
                     /locus_tag="MJF80_000344"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBZ0279026.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            33797..34471
                     /locus_tag="MJF80_000345"
     CDS             33797..34471
                     /locus_tag="MJF80_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBZ0279027.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVHNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            35024..35575
                     /gene="raiA"
                     /locus_tag="MJF80_000346"
     CDS             35024..35575
                     /gene="raiA"
                     /locus_tag="MJF80_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBZ0279028.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            35707..38382
                     /gene="secA"
                     /locus_tag="MJF80_000347"
     CDS             35707..38382
                     /gene="secA"
                     /locus_tag="MJF80_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBZ0279029.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            38397..39504
                     /gene="prfB"
                     /locus_tag="MJF80_000348"
     CDS             join(38397..38471,38473..39504)
                     /gene="prfB"
                     /locus_tag="MJF80_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBZ0279030.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            39648..41789
                     /locus_tag="MJF80_000349"
     CDS             39648..41789
                     /locus_tag="MJF80_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBZ0279031.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            41937..43103
                     /locus_tag="MJF80_000350"
     CDS             41937..43103
                     /locus_tag="MJF80_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0279032.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEEFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      43163..43280
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            43405..44010
                     /locus_tag="MJF80_000351"
     CDS             43405..44010
                     /locus_tag="MJF80_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBZ0279033.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     gene            44339..45379
                     /locus_tag="MJF80_000352"
     CDS             44339..45379
                     /locus_tag="MJF80_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBZ0279034.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            45518..45970
                     /gene="tsaE"
                     /locus_tag="MJF80_000353"
     CDS             45518..45970
                     /gene="tsaE"
                     /locus_tag="MJF80_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBZ0279035.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            45970..46686
                     /gene="tsaB"
                     /locus_tag="MJF80_000354"
     CDS             45970..46686
                     /gene="tsaB"
                     /locus_tag="MJF80_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBZ0279036.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            46676..47152
                     /gene="rimI"
                     /locus_tag="MJF80_000355"
     CDS             46676..47152
                     /gene="rimI"
                     /locus_tag="MJF80_000355"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBZ0279037.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            47158..48174
                     /gene="tsaD"
                     /locus_tag="MJF80_000356"
     CDS             47158..48174
                     /gene="tsaD"
                     /locus_tag="MJF80_000356"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBZ0279038.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREILSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            48471..51179
                     /gene="hpdB"
                     /locus_tag="MJF80_000357"
     CDS             48471..51179
                     /gene="hpdB"
                     /locus_tag="MJF80_000357"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBZ0279039.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            51183..51440
                     /gene="hpdC"
                     /locus_tag="MJF80_000358"
     CDS             51183..51440
                     /gene="hpdC"
                     /locus_tag="MJF80_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBZ0279040.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            51433..52383
                     /gene="hpdA"
                     /locus_tag="MJF80_000359"
     CDS             51433..52383
                     /gene="hpdA"
                     /locus_tag="MJF80_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBZ0279041.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            52699..53637
                     /locus_tag="MJF80_000360"
     CDS             52699..53637
                     /locus_tag="MJF80_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBZ0279042.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFTWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSNK"
     gene            complement(53759..54381)
                     /locus_tag="MJF80_000361"
                     /pseudo
     CDS             complement(53759..54381)
                     /locus_tag="MJF80_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
BASE COUNT        21917 a         5779 c        10302 g        16383 t
ORIGIN      
        1 agttaataag tggattatag aatttataat aattaatata tattaaacaa agatatatta
       61 agatatttta gaacacaaaa ctgcattaag gcgcgagtac tattttgtat tcgcgccttg
      121 atggtttgag ttatttgagt aaaaactaac tttaataaga aatctgtatt tattaagttg
      181 atatttttaa tgtgtttaga gattatttaa caatctccaa atataaaact agaattattt
      241 acataaaagt taaatagttt gtatagagtt agatttatat agtataatac ttagtgtaaa
      301 taattaagaa gttatcaaac aggaggataa aactgtgtta gatataaatt cttttttaaa
      361 tggatttttt aatataatat taagaatgag tatatatgat ttaattgaca tatctatagt
      421 agcgtatatt ttttacaaaa tatttatgtt tataaaagat acaagagcag aacaagtgtt
      481 taaaggtata atatttcttt tattagcaac tcaacttagt aacaccttta aattgcatac
      541 cgtttattgg atatctctta aagctttaga ttatggagtt atagctgctc ttataatttt
      601 tcaaccagaa tttagagcag gacttgaaca tataggaaga gcaaagttta atttatttgg
      661 aaagaatgta aatacttctg aagaaactct caatagaaat atagaagaaa ttgtagaagc
      721 tttatattca ctttctagac aaaaaatagg agctcttatc ataatggaga gagaaactag
      781 aataagtgat ataataaata caggaactat tattgatgct gaaatttcta ggcaactatt
      841 aataaatata tttataccaa atacaccatt acacgatgga gctgttgtaa ttagagattc
      901 taaggtaaaa gcagctgcat gttttttgcc attgacagaa agtaaggatt taagtaaaga
      961 tttgggtact agacatagag caggaatagg ggtaagtgag gtgtctgact gtataacttt
     1021 gattgtatca gaggaaacag gtggcgtatc aattgctaaa gcaggtaagt tatatagaga
     1081 tatttctaga gaaagaatga tgaatatatt acgtagtaat ttaaaaacaa atacagaaac
     1141 aagaagtttt ttcaaaggtg gtatatttaa atgataaata gactaaaaaa taataccaaa
     1201 ataaaaataa tatcattttt aagtgcaatt gcattatgga tgtatgttat ggctgtagtt
     1261 gaccctgaag aaactaaagg gtttgaagat atacctgtat ctataagtaa tatgagtgat
     1321 ttaaaggata aaaatcttgt tatatatcca gacgaaaaat taacagcaga tgtatatata
     1381 aaaggtagtt tatcaaaggt acaaagtgtt aaaaaagaag atatacatgt atatggaacc
     1441 attgaagacc caatacaagg acagaaggaa gtatatttaa aagcaaatat tcctcaaggt
     1501 gttactctag aatttaagca tgatgtttta gtagtaaatc ttgaaaaaaa cataaaagaa
     1561 aaaagagata taaaggtaga agtaagtgga aattcaaaaa gaaatataaa taaaattgaa
     1621 cttagtaagg aatttgtcga tgtatcaggt ccaagaactc tagttaaaga agtcaaaagt
     1681 gtaagagcta atctagatgt tggtaatgag acagatgatt tttctaaaaa attgcaatta
     1741 ataccattaa atagtgatgg aactaaggtt gaaggagtaa cattaagttc atcctttgtg
     1801 actgcaaaga tagaactatt gaaggaaaaa attgtcccaa taagagtaag tctaaatgat
     1861 aataaagata atgatgtact gaaaaactat aaattaagta aggaacaagt tacaataaaa
     1921 ggaaagaaag aagatataga caaaataagt tatattaaga caaaacctat tgacttagct
     1981 gatttggcta atggtgaaga aaaagaagtt agcttagaaa ttccagaggg tataactctt
     2041 gaggatagct ctataacaat taaattaaat gatacaaaac aattgagtgc agaattttta
     2101 tataatgcgg gagatattga attaagaaat atcccaacaa atatggataa tccaaatgta
     2161 atttctgatg tagatataaa agttgttgta gagtcaacgg aggatttgag taagttaaat
     2221 aaaaaagata tttcattata tattgattta tcacaaggcc ctgaggaaga tagtaaatat
     2281 aaaataaaat atgaaacaac atatcaattt aaaaaaataa atatagaacc taatattgtt
     2341 gaagtggaat gaacaaatat ttcgaatatt tagttgttac aagatgcgac aaaaaaacct
     2401 ataaaattaa gtttgttaat tttatgtttt tgttgcatct tgtttatttt agttatatta
     2461 taaaataagg acaatgagat atttgttaaa tctaaaaagc gctagatata tgaaaaaaga
     2521 ctgataaata gatttaaata aatattttga attaaatatt tcaattatct ctaggaaaac
     2581 gttgctgaac gagttataat ttaattgaga tataaaaagt tctatttcaa aaaattcaag
     2641 atgaggtgga aatgcatgag aagttttgaa gaagtaatta agtttgcaaa agaaagagga
     2701 cctaaaacta tatcagtagc atgttgccaa gataaagaag ttttaatggc agttgaaatg
     2761 gctagaaaag aaaaaatagc aaatgccatt ttagtaggag atatagaaaa gactaaagaa
     2821 attgcaaaaa gcatagacat ggatatcgaa aattatgaac tgatagatat aaaagattta
     2881 gcagaagcat ctctaaaatc tgttgaatta gtttcacaag gaaaagccga catggtaatg
     2941 aaaggcttag tagacacatc aataatacta aaagcagttt taaataaaga agtaggtctt
     3001 agaactggaa atgtattaag tcacgtagca gtatttgatg tagagggata tgatagatta
     3061 tttttcgtaa ctgacgcagc tatgaactta gctcctgata caaatactaa aaagcaaatc
     3121 atagaaaatg cttgcacagt agcacattca ttagatataa gtgaaccaaa agttgctgca
     3181 atatgcgcaa aagaaaaagt aaatccaaaa atgaaagata cagttgaagc taaagaacta
     3241 gaagaaatgt atgaaagagg agaaatcaaa ggttgtatgg ttggtggacc ttttgcaatt
     3301 gataatgcag tatctttaga agcagctaaa cataaaggta taaatcatcc tgtagcagga
     3361 agagctgata tattattagc cccagatatt gaaggtggta acatattata taaagctttg
     3421 gtattcttct caaaatcaaa aaatgcagga gttatagttg gggctaaagc accaataata
     3481 ttaacttcta gagcagacag tgaagaaact aaactaaact caatagcttt aggtgtttta
     3541 atggcagcaa aggcataatt ttaaggggga aataggcaaa tgagcaaaat atttaaaatc
     3601 ttaacaataa atcctggttc gacatcaact aaaatagctg tatttgataa tgaggattta
     3661 gtatttgaaa aaactttaag acattcttca gaagaaatag gaaaatatga gaaggtgtct
     3721 gaccaatttg aatttcgtaa acaagtaata gaagaagctc taaaagaagg tggagtaaaa
     3781 acatctgaat tagatgctgt agtaggtaga ggaggacttc ttaaacctat aaaaggtggt
     3841 acttattcag taagtgctgc tatgattgaa gatttaaaag tgggagtttt aggagaacac
     3901 gcttcaaacc taggtggaat aatagcaaaa caaataggtg aagaagtaaa tgttccttca
     3961 tacatagtag accctgttgt tgtagatgaa ttagaagatg ttgctagaat ttctggtatg
     4021 cctgaaataa gtagagcaag tgtagtacat gctttaaatc aaaaggcaat agcaagaaga
     4081 tatgctagag aaataaacaa gaaatatgaa gatataaatc ttatagttgc acacatgggt
     4141 ggaggagttt ctgttggagc tcataaaaat ggtaaaatag tagatgttgc aaacgcatta
     4201 gatggagaag gacctttctc tccagaaaga agtggtggac taccagtagg tgcattagta
     4261 aaaatgtgct ttagtggaaa atatactcaa gatgaaatta aaaagaaaat aaaaggtaat
     4321 ggcggattag ttgcatactt aaacactaat gatgctagag aagttgaaga aagaattgaa
     4381 gctggtgatg aaaaagctaa attagtatat gaagctatgg catatcaaat ctctaaagaa
     4441 ataggagcta gtgctgcagt tcttaaggga gatgtaaaag caatattatt aactggtgga
     4501 atcgcatatt caaaaatgtt tacagaaatg attgcagata gagttaaatt tatagcagat
     4561 gtaaaagttt atccaggtga agatgaaatg attgcattag ctcaaggtgg acttagagtt
     4621 ttaactggtg aagaagaggc tcaagtttat gataactaat gataaaagaa taagaatcat
     4681 aactggtcac tatggaagtg gtaaaagtga atttgcgatg aactatgttg ttaagttaag
     4741 agatatggtt tctggcaaag ttgctatagc cgatttagat gtggtaaatg tatactttag
     4801 aactagagaa aagaaagagt taatgaaatc cttaggtata caaccaatag atagctcaat
     4861 taatgctcca acattagatt taccagcagt atcagcagag gtaatgtctc ctatggtaga
     4921 ccattcatac aattctgtta tagatttagg tggagataat gtaggtgcta gggttatagg
     4981 aagatttagt catttgttaa aagaaggcga ttatgatatg ctttttgtaa taaatgcaaa
     5041 tagggagaaa actcaaactt ctgaagaagt tatccaatat ataaaagaaa tagaaaaatc
     5101 ttctaaatta aaggtaacag gtctaattaa taatactcat cttattaggt ttactactat
     5161 tgatgatgtt ttaagaggtc agaaagttgc aaaagaagtt tctgaaaaat gtaatatacc
     5221 gataagatat gttgcatgtt tagaaaatct tgttgaacag ttaccaaaag atttagaagg
     5281 agaaattttt cctataaaat tatatatgag agaagactgg atgtagtttg agctcaaata
     5341 tatagaaagt ttaaggaggc ttaggtaaat ggctaaagga aaagtatctt ttaatcaaga
     5401 acgttgtaaa ggttgtggat tgtgtgttga agcatgtcca gtaaaaatca tacaattaga
     5461 ctcaaatgta ataaacaaaa aagggtataa cccagcaact gtttttgaaa tggaaaaatg
     5521 cataggatgt gcaagttgtg caactatgtg tccagatgta gttataacag tagaaagaga
     5581 ttaatttaat taatatttaa gggggaattt taatggctaa gatacttatg aaaggtaacg
     5641 aagcatttgg aaaagcagct atagaagctg gttgcaaata tttcttcggt tacccaataa
     5701 ctccacaaag tgaattacca gaatatttat caagagagtt acctaaaata ggtggagcgt
     5761 tcgttcaagc tgaatcagaa gtttctgcga taaacatggt ttatggtgga gcaggtgcag
     5821 gagcaagagt tatgacttct tcatcttcac caggagttgc tttaaagcaa gaaggtataa
     5881 catatgcagt aggagcagaa gttccttgtg ttgtacttaa tgtaatgaga ggtggtccag
     5941 gacttggaag tatacaacct tcacaagctg actactttat gtctacaaga ggtggaggaa
     6001 atggagacta cagaactcca gtattcgcac cagctacagt tcaagaagct gttgatatga
     6061 taatggaagc ttttgacgtt gcagattatt acagatcacc agttatggta gttgcagatg
     6121 gtatgatagg tcaaatgatg gagccagttg aatttagagc tcctgagaag aaaagagaat
     6181 tgcctcctaa agattgggca actgttggaa ctaaaggaaa aagaaaacct aatgtaataa
     6241 actctctata cttagagcca gaagtactag aagaccattg ttggcattta caagaaaaat
     6301 ttgatgcaat ggaaaagaat gaagtacaat acgaaatgta caaaacagaa gatgctgaat
     6361 ttgtatttgc agcttatgga actacttcaa gagtagtaaa aagcgcaatt gatatattaa
     6421 gagaagaagg aataaaagca ggtcttataa gacctaaagt attatggcca ttcccattcg
     6481 aagcattcaa ccaaattcct aatgctagaa acatattgac tgttgaaatg agtatgggac
     6541 aaatggtgga agatgttaaa atggcagtag aaggtaaatt accagtttac ttccatggaa
     6601 gaccaggagg aatgactcca acaccagcag aaatagttga aaaagctaaa aaaattattg
     6661 ccggagaatt ggttgcagga ggtgctagat aatggctgtt gtatttaaga aaactgaagg
     6721 attacaagat actcaaactc actactgtcc aggatgtaca catggtataa tccatagatt
     6781 agttggtgaa gtattagaag aactaggagt attaggtgat gctgtaggtg ttgttccagt
     6841 tggatgttca gttttaggat ataaatattt taactgtgac actcaagaag ctgctcatgg
     6901 tagagcgcca gctgctgcta caggaataaa aagagttcat cctgaaaata cagtattcac
     6961 ttatcaaggt gatggagact tagcttcaat tggtactgct gaaatagttc atgcagctgc
     7021 tagaggagaa aaaataacta ctatatttgt aaacaataca acttatggta tgactggtgg
     7081 acaaatggct ccaactacat tagttggaca aagagctact actgctcaat caggaagaaa
     7141 tgctgaaact caaggttatc caataagagt aagtgaaatg ttagctacat taacaggagc
     7201 tgtatttgta gaaagagtag cagttgatac tccagctcat gttagacaag ctaaaaaagc
     7261 tataaagaaa gcattccaag ttcaacaagc aggattagga tttggaatag tagaagtatt
     7321 atctacttgt cctacaaact ggggactagc tccaaatgat gctttacaat ggttaagaga
     7381 caacatgata ccatactatc cattaggaaa tttcaaaaat gttgaagttg aggaggtgaa
     7441 gtaaatgtct acagctagag taatatgtgc aggcttcgga ggccaaggtg ttatgtctat
     7501 gggacaatta cttacttatg caggaatgct agaaggaaaa gaagtttctt ggttaccatc
     7561 ttatggacca gaaatgcgtg gaggtacagc aaactgtgca gtaactgtat ctaatgagcc
     7621 agttggttct ccacttataa cagatgatgc aactgcagct atattattaa atataccagc
     7681 atttgaaaaa ttcaaagatg atgttattcc tggtggaaaa ataatagtaa atagttcttt
     7741 aataaaagaa aaagttgata gaacagatgt agatgtttat tatataccag caaatgaatt
     7801 agcagctgaa ttaggaaatg ataaagttgc taacatgata atgttaggcg cttacttaaa
     7861 agtatctgat actgtagata tagaatctgt actagaagca ttcaaaaaag ttttcggacc
     7921 tagaaaagaa aaattcgttc cattaaacag agaagcttta caaaaaggta tggatgctac
     7981 atgttctaaa gcaactaact aataaagaaa aagatgtcaa aaaaaagaca atagaattat
     8041 ttctattgtc ttttttgctg ataagtatta aaatattttt atgactgaaa ttaagcctat
     8101 aatgatattt tatgaactat aaaaatataa tacaaattac aagcttttca attatgaaag
     8161 gagaatcttt agtgagaaag tattttggaa cagatggagt tagaggagta gcaaatacag
     8221 aactaacatg tgatttagca tataaacttg gtagagctgg tggatttgtc ttagctcaag
     8281 gagaccacag agtaaaagta gtagtaggta aggatactag aatatcaggt gatatgttgg
     8341 aagcatcttt aatagcagga cttatgtctg ttggatgtga tgttattact gttggaataa
     8401 taccaacacc tgcagtggca tatttaacta gaaaatatgg tgctgattgt ggtgtggtta
     8461 tatctgcatc tcacaatcct gttgaatata atggaatcaa attctttaat aagaatggat
     8521 ataaattaga tgatgaaatt gaattgaaaa tagaagaata tatagacgat atagacaaaa
     8581 ttgattgtct tccaattggt gaaaatgtag gaagaaaatt acatgaacat tgtgcacaaa
     8641 gagactatgt agactaccta aaatcaataa taagcactga ctttaaggga ttaaaagtag
     8701 ttttggactg tgctaatggt gcgtcatata aagtggcacc aatagtgttt gatgaactag
     8761 gagcaagtgt aatttcaata aatagtttac cagatggaaa taatataaac tataaatgtg
     8821 gctctacaca tccagaacaa cttcaaaggg ctgttttgga acataatgct gacttaggtc
     8881 ttgcatatga tggagatgct gatagattaa ttgcagtaaa tgaaaagggt caaattgttg
     8941 atggagacca tataatgata ttgagtgcct taaatttaaa gaaaaataat aaattggcac
     9001 aagatacttt ggtagttaca gttatgagta atatagggtt gacaatagct gctaaggaaa
     9061 atggaataaa tttatctact acagcagttg gagacagata tgttttagaa gatatggtta
     9121 aaaatggata taatcttggt ggagagcaat ctggacatat gatattttta gattacaaca
     9181 ctactggaga tggagtgctt agttctctaa tacttgcaaa tataattttg caagaaaaga
     9241 aacctttatc agaaattgca tcaataatga gccaataccc acaagtatta gtaaatgcaa
     9301 caataaaaaa tgaaaataag aacaaatata tggaatatcc agaaataaaa actgagatag
     9361 aaagaataga atctatttta gacggaaatg gtagagtttt gataagacca tcaggaactg
     9421 agccattagt tagagttatg ttagagggta aagaggaagg tcaaataaaa gaattagcaa
     9481 cagacttagc taatcttata caagaaaaat tatcgtaatt taaagggtat tctcagatac
     9541 aaatactgag gacacctttc taagcgccag aacttagtta tagacataat tattgagtaa
     9601 tataggaact aagttgacga ggtcagagtt aatcgaattg tcggcggatg ctctgcggtg
     9661 tgtttaccat cgaaagactt ctttaaataa taggagcaat cctatggaca aagggaagcc
     9721 aaagtaaact tatatgacct aactcttaaa atatataaat aatattttta ggaggacaca
     9781 aataatgtgt ggaatagttg gatatttagg aagtagaaag gcagcagaag ttatagtaga
     9841 agggctttct aagctagagt acagaggtta tgactcagca ggagtagcag ttaatagttc
     9901 taatgaaaaa gaacttaata ttagaaagtt taaaggtaga ctttctgtac ttgcagaaga
     9961 cttagaaaag aatcctattg atggaaattt aggtataggg catacgagat gggcaactca
    10021 tggagaacca tctgatgtaa actcacatcc tcattttaat caagccaaaa caatagcagt
    10081 tgttcataat gggataatag aaaactatat ggaaataaaa gaagaattaa tttctgaagg
    10141 tgttaagttt gaatctcaaa cagatacaga ggtaatagca catttagtag ataaatacta
    10201 tgagggtaat ttattagatg ctgtatacaa aactatatca aaattaagag gagcttatgc
    10261 attaggtgtt atatgtaaag aacatggaaa tgaattagtt gcagtaagaa aagatagtcc
    10321 tcttgtagtt ggtgttggtg aaggtgaaaa ctttatagca tcagatatac ctgcattatt
    10381 aaaatatact agagatgttt atttcttaga aaatggagaa gtagttcatc taaaagacga
    10441 aaatgtaacc gtttatgact ctaatagaaa tcttgttgaa aaagaagtat ttcatgtaac
    10501 ttgggatgta gaagctgcgt ctaaaggtgg atatgactac tttatgtcaa aagaaattca
    10561 tgagcaacca acaggagtaa gagaaacttt agaaagaaga cttgatgata atggaaatat
    10621 aattctagac agtataaata tttctaaaga agacttagaa aaaataaata aagtttatat
    10681 agtggcttgt ggaacagcat ataatgctgg attacttggt aagtatgcca tagaaaaatt
    10741 tgtaaatata ccagtaataa ctgatatagc atcagaattt agatatagcg acccatttgt
    10801 agatgaaaat tcattagtaa tattagttag tcaatctggt gagacagcag atactctagc
    10861 agtattaaga gattcaaagg ctaaaggagc aagaatactt tcaataacta acgttgtagg
    10921 ttcatctata gcaagagaat cagatgatgt attctatact tgggcaggac ctgaagtagc
    10981 agttgcatca actaaggcat atactactca aataacttct ttatacatga tagctttaga
    11041 ctttgctatc aaaaaaggaa caataactag agaattttat gatagtatga tttctaagat
    11101 gaaagaaata ccatcaaaaa tacaagaaat actagacaat gaagaatata taaaagaagt
    11161 tgctaaaaca gttgttagtt ctgaacatgc attctattta ggaagaggta tagactatag
    11221 cttagcaatg gaaggttcat tgaaattaaa agagatttca tatatacatg cagaagcttt
    11281 tgcagcagga gagctaaagc atggaacaat agctttaata gaaaaaggaa ctcctgtaat
    11341 tgccatagct actcaagaaa agttatttga aaaaatggtt tctaatatgg aagaagtaag
    11401 agcaagagga gcttatgtag tagcaatagc tcaaagccat aataaggatg ttgagaaagc
    11461 tgctgataaa ataatctata taccaaattc agatgatatt ttatcaccaa tattagcagt
    11521 tgtaccaatg caattacttg cttatcatgt ctctgtacta agaggatgcg atgtagataa
    11581 gccaagaaat ctagctaaat cagtaacagt tgaataaaaa cagattaaaa attaaataga
    11641 taatatttat tgaaaagaac tcatcctgtt atttgtattt tataggatga gttttttaga
    11701 taaatattaa atttgttttg tttttgctat aataaatttt ataaaaagat tgatattaaa
    11761 tcatctgtaa aatgataaat atatagacca taccaggtat agggaacgat aaatatagtt
    11821 catataaaat atagtgagta cttaataaac atatagtcaa tatcagataa aaaaaataat
    11881 agcttatgag aaaaattaaa atacaaggta ttagtaacct tgtgataaga taaagtgagt
    11941 agtttaaaat aagaatataa ggaactattt ataaaagaat ataatgaagc taatagagta
    12001 taagaaaatt actttatttt agtaaaataa tttcaaataa aaattttttt aaattgggag
    12061 tataaattat gacaaatgat tttaaaaaag aaaagaaaga agattatttt gttaatctta
    12121 aggcaatatc tactgaagtt ttacaagaaa aagtgcaaga taacgcttgg gttatagttg
    12181 atactcgctt aaatgatgct tataatggct ggaagttaga tggagtaaaa agaggtggac
    12241 atataaaagg agcagtagat ttttctgcta attggttaag tgtatactta gatagaaaag
    12301 atgaagtgtt agaacaagca ttgaaaacta aaagaattga tttagataaa aatatagtat
    12361 tgtatgatgc aaatggaaaa gatgctttgg ttgtagcaga ttatttatct aaaaaaggat
    12421 ataaatatct atataaatat gatattaaac aatgggcaga cgacgaaaat cttccaatgg
    12481 aaagatataa aaattatcaa atgatagttc ctgcttttat aataaaagat atattagatg
    12541 gaaaaattcc tgagacattt gaagattcaa agaatataaa aatgattgaa gcgagttggg
    12601 gagaagaatc gtatacaaaa gggcatatac ctacaagtgt tcatgtaaat actgatatta
    12661 tagaacctcc tccaacatgg atgcttgata atgatgataa cttaactaag tttgctttag
    12721 actatggatt aacaaaggat gatactgtaa tagtttcaag tagtacacct atggcatctt
    12781 atagactagc tgttatattg agatatatag gagtaaaaga tgtaagagtt ttgaatggtg
    12841 gaacaaactc atggttatca gcaggatatg aattagaatt tattagtaat ccaaaacatt
    12901 catgtactaa ttttggagca gatataccag ttaactcaca attagttgtt actacatctg
    12961 aattaagaca aaaactaaaa gaaaaaaata agtttatact agtagataac cgtacttggg
    13021 atgaacatat aggaaaagta tctggatata catattatga taaaaaaggg cgtataccag
    13081 gagctttata tggacattca ggtagtgata gtgtttcact agaagaatat cgtaatattg
    13141 ataatacaat gagaaataaa tatgagattt tagaaatgtg ggacaaggaa aatatagatg
    13201 tgaataaaca gcttatattt atgtgtggaa gtggatggcg tgctgctgaa gtattaacat
    13261 atgcaaatgt cataggagtg gaaaatacct ccctttatag tgatggatgg atgggctgga
    13321 gtcttgataa ttcaaattta attgaagtgg gagaacacaa ataaagtttt tgaaagtaca
    13381 atagagttgt ttaaaacaaa ggtgtttttg atttattagg aagagtgtgt aaattgtgta
    13441 ttgggaagtg gacttaggta tgttcatttg gagctatgga aactgataaa aaagtattat
    13501 ctgtttattt tgctggtaaa atatgaatag attatagatt atagattata gattatagat
    13561 tatagattat agattataga ttatagatta tagattatag atttaagtat agactaagta
    13621 atttatatta cagaagagaa tatataagaa ttaatgcaag atgtatttga tacatataag
    13681 aaattggcaa gtccaggaga aggatagaga aaattgagat tgaagagttt aaaagagatt
    13741 attagatgaa aaatacaaaa atgtataaaa atacatattg agaaagtata tctctcttaa
    13801 atttgttaat aattctatta tacagaaaaa catatttaag ggggattttt tactatgaaa
    13861 aaagtaaaat tagctataag cttttttata ttaatgatta taggaatgac aatatcttat
    13921 gcagatacaa gtataaatac tagatacaat agattaagta aagctcttga aatatcagaa
    13981 gcaaatttta aattttataa tgttaaaatt aattctgtta taaatcataa tttatcaaat
    14041 aaagaatttc ataatatttt tatagaaata attaacaatc taaagttgga taaaaaagga
    14101 ataaattgga atcaaaaagg gtctggcgat aaaatacaat tatgtgctac tataaaaggt
    14161 agcgataaag aaatttctat aaaaggaata aataaaaata acaaagaaag ttacatagta
    14221 gttgacatat tggataacaa agtatataaa cataaagagg ttatttatag taacgtagaa
    14281 aatgtgttaa ataaatattc atctcaagta gatgtgtctg cttgtattgt tggagaatac
    14341 acaaaaaggt tacaaaacaa caaatatgat gatattttac aaaaaatatt atataatatg
    14401 agtgctaagc aaatagatga agttaaagac gtaaactttt tatctataac agcatatagt
    14461 aaagtattaa atgattatga attagaatat ttaggggaaa aaattaattt aaatatagga
    14521 attaaatata atgaggatga cggaaaaaca ctcatatata ttgccacacc aattataaaa
    14581 ttagattatt aattatgagg agatcaaaaa atatggctaa gataatagta aaaaagagta
    14641 atccacttaa aggtagtgtt aaaatagatg gtgcaaaaaa tgcagtttta ccaataatag
    14701 cagcgacttt attagcaaac ggaaaatcaa cattaaatgg agtaccaaat ttaagagatg
    14761 ttcatgttat atcagattta ttaagacatg taggagcaga agttgaatat aaagaaaata
    14821 cacttactgt tgatgcaagc aatataaaaa cttgtgaagc tccatatgag cttgtaagaa
    14881 agatgagagc ttcttttttg gttatgggac cattgcttgc tagatttaat agtacgaaaa
    14941 tttctatgcc aggaggatgt gctataggaa caagacctat agatttacat ttaaaaggat
    15001 ttaaagcctt aggtgcaaag atagaaatgg accatggttt tgttgaagca gcaacagaaa
    15061 agttagtagg aaataaatta tatttagatt tcccatctgt tggagcaact gaaaacataa
    15121 tgatggcagc ttctttagca gaagggacta caataataga gaatgcagca gaagagccag
    15181 aaatagtaga tttagcaaac ttcttgaacg aaatgggagc agatgttaaa ggtgcaggta
    15241 ctaatactat aaaaataaaa ggtgttaaag agttaaaagg agcagaacat aatgtaattc
    15301 cagacagaat agaagcagca acttatatgg ttgcagcagc tatgacaaaa ggtgatataa
    15361 ctgttgaaaa tgttttaatg gagcatttaa aaccagttgt agctaaatta agagaagctg
    15421 gttgtgaaat aacagaaatg gacaattctg taagagttgt aggtccaaaa gtattaaaac
    15481 caatagatat aaaaacttta ccacatccag gatttcctac agatgttcaa gcacaattta
    15541 tggctatgct tactgtagcc aatggaactg gtgtagttat agaaactgtt tttgaaaaca
    15601 gatttatgca tgttgctgaa tttaatagaa tgggtgctaa tataaaaata gatggaagaa
    15661 gtgcggttgt taatggtgta gatgagctac acggtgcagc agtaaacgct actgatttaa
    15721 gagcaggggc agctcttatt ttatgcggtc ttatagcaga aggagaaact caaataggag
    15781 aaatatacca tatccaaaga ggttatgttg atatagataa gaagataaca gcactaggtg
    15841 gtcaaatcga aatagtagaa gattaataat tggtttatag aagaaagtta taaattaata
    15901 gggttgttca tatacttaaa tacaaggttt taaaagctaa ggaggagagt atatgaagaa
    15961 cccattggtt gttttgttag gatttgtgac ttgctctgtg ttagttccat cacttataac
    16021 tttagtttct tataaaaatg tagaattaac tgaaaaacca gagagcccag tatctataaa
    16081 caaaactatt aaaaaaagtg atattgaaga caaaggtaat aaagaagaaa aaaatacagt
    16141 gaactatgaa actgtaaata aaaaagcacc aattatcaat gtatataatc atataacagg
    16201 gaaaaccgaa aagatggaca tggagaacta tttatgtgga gtactagctg gtgagatgtc
    16261 ttcagagttt gatatagaag ctttaaaagc tcaatctgta gcagctagaa catatgtagt
    16321 atataaacag gaacatggta aatctagtaa acataaaaat gcagtagtat gtactgatta
    16381 taaacattgt caagaatata agagttatga tactcttaaa aaattaaatg gtgaagaatg
    16441 gattaagaat aaatattcca aaatccaaga agcagttaga ggaactaaag gtcaaataat
    16501 aacttataat gataaggcga tacttcctct ttatttttct acatcttcag gaaaaacaga
    16561 aaatagtgaa gaagtatttt ctgcaaaata tccttattta aagtctgtgg aaagtcctta
    16621 tgataagtat tcccctaaat ttgcgtcaac acttaaaata agtaacacag attttgttaa
    16681 aagtcttaga agggcatata gcactattgt gatagatgtg aataatttaa gtaaacaagt
    16741 ttcaataact aaaagaagtg atgccggaac tgtagaaaaa ataaaattag gaaataaaga
    16801 attgactgga aaagacatta gaactgtttt taaattgaac tctgcaaact ttgacataaa
    16861 atttggagaa ggatacatag atttcgtggt aaaaggatat ggtcatggtg ttggtatgag
    16921 ccagtgggga gcagaaggaa tggcagaaga gggctataaa tattatgata tattatcaca
    16981 ttactacaca gatacaaaaa taaaagatat atactaataa ttgtataaaa tgccaaaaac
    17041 acgtcaataa tccaaatgta gaaatatatt tggaggtgca aaatgaagaa aaagctgtta
    17101 gaaaaagatg gtttttattt atctttgttt gtatgtgttt gtttattagc agtaggtgga
    17161 gtttggttta caaataataa tgtagataaa ttggcttcta ataaaggaat tatggaaaat
    17221 gctaataaag atagtgaaga ggaaatacat ttaattgaaa aagacaagaa agatgctatc
    17281 cctactgcaa cggattcaaa acaaaactta gaaaaagcta agtcaaaaga ggaaaataaa
    17341 tcgagtacaa caaaattaaa ttacattgga gataaagtaa taagaggata ttcagaaaaa
    17401 gagcctagtt attctaaaac acttgatgtt tgggaaactc ataaaggtgt agatattagt
    17461 tgtactaaag gcaaagaagt aaaatctcta ttaaacggaa tagtagtaga tgtatttgat
    17521 gatgaagaat atggacaatc agtcaaaata aaaagtgaca ataacattgt agtagtatat
    17581 tcaaatctag ataaaaatgt tagtgttaag aaagaacaga aggtaacaga aggacaatct
    17641 ctaggtactg taggtagtac ttctcaaata gaaagtgaag aaggtataca tgtgcattta
    17701 gaggcgtata gtggagaaaa atctatagac ccaatgagtc taattaagta atagtataac
    17761 aagtttaatc atatttggtt aagcttgtta tattttttgg tgtttttaca tatatatcat
    17821 taaagctatt atgtgagggg ggtaatcttt gagatctcat atagaggaaa gggccatagt
    17881 ggtagcaaaa tatatacttg aaaaaaacac tacagtaaga caaaccgcca agacatttgg
    17941 agtaagtaaa agtacaattc ataaggatgt tacagaaaga ttgaaggaaa taaatccatc
    18001 ccttgccaaa gaagttaaaa atgtgctgga caaaaataaa tcagagagac atattagagg
    18061 gggattagca acgaaattaa aatatgagaa ggaacataaa aaaatgtagg ttaaaaacct
    18121 acttttttta ttgaattaaa ttataaaaag aaatatatta agtaattata aatagcaaaa
    18181 taataatact ctcaaaagat ttattttctc ttgattaaag cgagattttt gtatatgctt
    18241 tattatagga gtattaaaat aggcttataa tataatctaa tattataaat aaaggagttg
    18301 aaaaaatggc tggagctgat ataggcatag atttaggtac tgcaaatgtg cttgtttatg
    18361 ttagtggtaa gggaatcgtt ttagaagaac catctgttgt ggcgatagac aaaagaacag
    18421 attctgtttt agctgtggga gaagaagcta aaaagatgat agggagaaca ccaggtaata
    18481 tagtcgctat aagaccttta agagatggtg taatttcaga ttatgatgta actgagaaga
    18541 tgttaaaatc ttttatagat aagatagttg ataaaaaggg atttggaaga ttttttatgc
    18601 caagaattat ggtatgtgta ccaacaggtg tcacagaagt ggaaaaaaga gcagtagaag
    18661 atgctacgag acaagctgga gctagagaag tatatatcat agaagaacca atagcagctg
    18721 ccataggagc tggtgtagac atatcacagc ctaatggtaa tatgattata gatattggtg
    18781 gaggtactgt agatatagct gtaatatctc taggaggagc tgttgtaagt gaatctataa
    18841 aggttggtgg agatagattt gatgatgcta tagttaaata tatgaaaaaa caacacaatc
    18901 ttcttatagg agaaagaaca gcagagaaaa tcaagtttga aataggttct gcatttaaga
    18961 gagaagaaga aaaatatatg aagataacag gaagaaattt aattacagga cttcctagtt
    19021 ctataactat aaattcaaca gaaatgttgg aagcacttag agaatgtgtt gagcaaatag
    19081 tagttgctac tcatgctgtt ttggaaaaaa cgcctcctga gttagcagca gatataggag
    19141 atgtcggtat aataatgact ggaggaggtt cattattata tggattagat aaaataatag
    19201 aacagaggac aggaataact gttacaatag cagatgaacc attatcttgt gttgcaaaag
    19261 gtacaggaat agctttaggt tcaatagact tacttgaaac tggtgggtct ttcaaaagaa
    19321 aataattagg agagtttaga atgttaaata tagatgaaat aaaaaaatta ataccacata
    19381 gatatccatt tttattagta gacaagatta cagagctaga agttgggaaa agagctgttg
    19441 gtataaagaa tgtaacagtt aatgagccat ttttccaagg tcactttcca gaatatcctt
    19501 tgatgccagg agtactaata gtagaagctc tagcacaagt atgtggagtg gcaatgatgt
    19561 ctgtagagga aaacaaagga aaactaggtg tatttgcagg tatagataaa gttagaatca
    19621 agagagaagt aagaccagga gatacattaa ctatggaagt tgaaatgaca actcttagaa
    19681 aaaatatagc taaggcagat gctaaggcat atgtaggtga agaacttgtc tgtaaaggtg
    19741 aattaatgtt tgctttagta gaaaaataaa aacttgaaat ataaaagtaa aaaggtagat
    19801 aatttggagt atttaatcag atactctaaa atatctacct ttttatataa agaattttat
    19861 catttagttt atatttagat taggtggata ctaataaagc caaggctatt gtttcagcta
    19921 aatctaatat aaagctaagg cgtatattat taaatgaaag tttattattt tcatcaattt
    19981 tatctacaat tcctactata gaataatttc ctatttgagg tagtttttta ccaacacctt
    20041 ttccgggcag gataggtcct tctctaatct gtatattacc tatgttactc tgcgacccca
    20101 agcaagcatc tatagccaag aagttacctt gtatatgtgt attttttata gtatctaaag
    20161 actcatatat atttaaggcg tgaatagggt tatctagtgt accgtaaacg gggtatttaa
    20221 agttactgtt cttaagtatt gttccaacta aagggcctaa agtatcacct atagccctgt
    20281 ctgttcctat gcaaacaaca atcgtatttt cattaattat agtttttaga actgaactta
    20341 gcatctgtat aacatcatca tcgctgtagt ttactttagc taaatacaat aggaacaccc
    20401 ccttcaaaaa aatatatgaa taaaaagcat taaaaagacc taactttaaa tttcagatag
    20461 atagaaaatt ttatttgtct tacaaaaaaa ggaatagaaa aaatatgact aaaaatattg
    20521 acaaaatttt aaaaaaatag taagctagta aagtaaaaca aaacttaaat cttgattacc
    20581 ttatcaagag aggctgaggg ataggccctg tgaagcccag caaccaccca aatgggaaaa
    20641 ggtgctaatt cctacaagac aaaagtcttg aaagatgagg tcagttaatt agtatgtaat
    20701 tacgattatt aacctcttct tataaaggag aggttttttt attttgagta aatttctaaa
    20761 attaaaaata aggtaaaacc tataataaaa attaagaata aataaggggg aataaaaatg
    20821 gcaagacatt tatttacgtc agaatcagtt acagaaggac atcctgataa gatatgtgac
    20881 caaatatcag attcaatatt agatgcatta ttagaaaaag acccacaatc tagagtagct
    20941 tgtgaaacta cagttactac tggtttagtt ttagtagcag gagaaataag tacatctgct
    21001 tatgtagaca ttcctaaatt agtaagagaa acagtaagag aaattggata tacaagagca
    21061 aaatatggat ttgattgtga tacttgcgca gttataactt ctattgatga acaatctgga
    21121 gatatagcta tgggagttga tgaaggtcta gaaagtaaga ctggagaaga gatagaagaa
    21181 gaaatagaaa aagttggagc tggagaccaa ggtataatgt ttggatttgc atgtaatgag
    21241 actccagaat taatgccact tccgatatct ttggcacata aattatcaag aagacttaca
    21301 gaagttagaa aaacaggttt agttgattat ttaagaccag atggaaaaac tcaagttact
    21361 gttgaatatg aaggaagtaa agctgtaaga gtacatacag ttcttatatc agctcaacat
    21421 tgtgaaacag tatcaaatga taaaataaga gaagatttaa ttaatcatgt tattaaggaa
    21481 gttataccag cagaattact tgatgaagag actaaaattt atataaatcc aacaggaaga
    21541 ttcgttatag gaggaccaca aggagataca ggtcttacag gaagaaaaat aataatagat
    21601 acttatggtg gatattctag acatggtgga ggagctttct caggaaaaga ccctacaaaa
    21661 gttgatagat ctgctgctta tgcagctaga tatgttgcaa aaaatattgt tgcggctggt
    21721 cttgcagaca aatgtgagat agaacttgca tatgctatag gtatagctag accattatct
    21781 atatttatag atacttttgg gacaggcaag gtttcagaag aaaaacttgt tgaattagta
    21841 aataaacact ttgatttaag accgggagcg ataataagag acttagattt aagaaagcct
    21901 ctttataaaa aggttgctgc ttatggtcac tttggtagaa ctgatataga tttaccttgg
    21961 gaaagaactg acaaggttga gcagttaaga aaagatgctt taggtgaata atttttaact
    22021 aaaaagctgt tagaatgtaa ttttatgatt actttctaac agctttttta ttatgagtta
    22081 aatattttta ataaatgtta atttatcatc atataatgaa taaacagtat ttaatctggt
    22141 caatacattt tatataatac tctactaaat ttatacatat tatgggatat atgttaaaga
    22201 atggttaaga atgtgttaac taatgttaaa taattaaaaa tatattgaaa tatcacaata
    22261 atgagtctaa tataatctta ataaacttac actttaaaaa taattttaat tgggggggag
    22321 tagtgtatgt caggcaaagt aaatattaaa acggttataa gttttgctgg agcttatgtt
    22381 gctactgtaa taggttctgg atttgcaact ggtcaagaaa tactgcaatt ttttacgttt
    22441 tatggatatg cgggtattat aggtggaata atctctatgg tactattttc atggtttgga
    22501 gcagaagtta tagataaggg aagagaattg aaacttaagg agccaataaa aatttatcaa
    22561 gtatattgtg gaaaatatct tggaacattt tttgaatggt ttggtccatt gtttttattt
    22621 ggaacattag taataatgat tgcaggagca ggagctacat tatcagagta ctatggatta
    22681 aatccatatg taggtaggat tggtatggca atcgtttccc taataactgt ttcattaggt
    22741 ttgacaaggc tttctaaaat actaggtaat ataggaccta taataataat atttacactt
    22801 ttagtaggag caattagttt atttagcaat attgatgctt tatcaaatgc tggaaatatg
    22861 gttgatagtt taaatattaa aactgctaca tcaaatggat atttctcggg agtactttat
    22921 acttgttaca atgtaataat agtaataaca ttcttgacag gaatgggagc ttctgcagta
    22981 aataagaaag atgctgtatg gggtggtata gtaggtggag tagcacttat ggcagcagct
    23041 ataatgatga atttggcatt actttcagac ataggaaata tatacacaaa agagatacca
    23101 gcactttact tagctgataa gatatctcca gtaataggta ttttattctc agtagtactt
    23161 ttactaggaa tatacactac agctgtacca ttgttatggt cagtaacaaa ccgatttgta
    23221 gaagatgacc accctaagtt taaaattata acaatagtgg taagtatatt agcttgtata
    23281 ggaggtttac tgccatttga taagttagta ggtacactat atccatatac aggttacatg
    23341 ggaattttaa ttttactatg tatgctttat agaagaatta ctaaaactga ggggtacaag
    23401 gagaataaat ctgaaataag ttagtactgt aatatactta tgaaatttaa tttatactct
    23461 aaatatagag taattaaaat aaaatagcta tttaaatatg gtacctgtat ttcatggtac
    23521 catattttat ggatgcagat tttttttgtt tctgataaaa tgtataatag ttggaaatta
    23581 cgttaagtat aaaaatataa aacttttcta cgattatcat ggttttaata tataaaatta
    23641 tactaacatt taaatggagc gattattagt atttttagga gataaaaatg gaaaaattag
    23701 aaggaatgat aagtgaaata gtctttaaaa acgaagacaa tggttataca atagctcacc
    23761 tggtaaatga aaatgatgaa attgtggttg taggatgtat gcctactttg gcaattggag
    23821 aaagcataga agttgaagga aagtgggtaa atcataagat ttatgggact caatttgaag
    23881 tgaatagttt tatgccagta acaccatcat ccttagaagg aatttatgtt tacttatctt
    23941 ctggaatgat acatggtatt ggtgaaaaaa tggcaaaaag aataattgat aagtttggtg
    24001 tagatacgtt agaggtgata caaaattcac cagaaaaact acaagaagta gaaggtatag
    24061 ggagtaaaaa agtaaaacaa atagtaaaaa gttatgaaga agacagagaa ttgagaaata
    24121 taataataca gctatctcct tttggaataa caccaaatta ctgcctaaaa atatataaaa
    24181 aatataaaag cagtgcaata gaagttataa ataaaaaccc ataccaactt gctgaagata
    24241 taagaggaat tggatttaaa gtagctgata gtatagcaag taaaattggt atagataaaa
    24301 actctaaaga tagaatttgt cagggaatct tatacacttt aaataaatct ttaagtaatg
    24361 ggcatacata tttaccagag catgttctaa tacaagattc ggaaaaattg ttggagctaa
    24421 atggagaaat actaaaagag tgtgtaatga tgctagttta taatcaaaaa atacatatag
    24481 agaaagtaaa caatgaaaat cttatatacc ttatgccata ctatttagct gaaaatggag
    24541 tatgtagtca aatagtaaaa ttatcacagt atgaatttga agacttaaaa atagatatag
    24601 ataacgagat taatgtttta gaagaagata agaaaattaa gttagcagaa aaacaaattt
    24661 tagcagtcaa agaatctgtt aatagtggag tattaattat aactggtgga ccaggaactg
    24721 gtaaaacaac taccattaat gctatcatag atatatttga aaataatggt aaaagtgtga
    24781 ctttggcagc tccaacggga agagcagcaa agagaatgag tgaaacttct aataaagaag
    24841 caaaaactat acataggttg ctagaaatgg gattttcaac agatgatgat ttaacttttt
    24901 ttaaggatga agaagaccca ataaattcag atgtaataat tgttgatgag gtatcaatgg
    24961 tagacattat cttaatgtac aatttactta gagctataaa gttaggaaca agagtaattt
    25021 tagttggaga tagtgaccaa ttaccttctg taggtgctgg aaatgtactt aaggatatga
    25081 ttaattctaa tattataaat gtagttaaat taaatgaaat atttagacaa gcgcaagaaa
    25141 gtatgataat tgtaaatgcc cataagataa acaatggaga accactttat ctaaatacaa
    25201 aaggtaaaga tttctttttt ataagaaaat caactaatga agaaatttta aatgaaataa
    25261 taggattggt taatgaaaga ttacctaaat tttataaagt tgataaatta aaagatatac
    25321 aagttttatc ttcaatgaga aaaggtgaat tgggagttac taatctcaat atagagttac
    25381 agaagtatct taacaaaaaa gaaaaattta aagttgaaga aagtttttct aaaagattat
    25441 ttagagttgg cgataaggta atgcaagtaa aaaataatta tacaaaaaaa tgggaaacag
    25501 aagaccaaaa agaaagtgga gaaggtatct ataatggaga tataggatat gtttatcaca
    25561 tagataaaga taagaaaact atttatatat tatttgacca gactaaaatt gtatcatacc
    25621 tatatgatga attagatgaa attgaccata gtttttgtac aacaatacat aaaagccagg
    25681 gaagtgaatt tccagttgta gtattgccaa tagcgtgggc tcctcctatg ttacttagcc
    25741 gtaatttatt atatacagca gtaacaagag caaaaaaatt agttgtatta gttggagatg
    25801 tgaaatatct tgaatatatg ataaaaaata atagagttaa tcaaaggtat tcaaatttag
    25861 gatataagct aaataaattt aaacaagaag ggttgttgat agaataaata tgaaaatatg
    25921 caatgataat tttattaaga attttaaaaa tatgattaat aaatgtttgg attttatata
    25981 tcctgagaat ataagttgca taatttgtga taaatccata aagaaaacaa atacttattc
    26041 aatatgtaaa agttgtttta aagaaatgaa ttttatacaa gatggttgca tgaagtgtgg
    26101 aaaaccaata atacgtcatt caattgaaaa agaatttatt gaagagtgta gttattgttt
    26161 taataaagac ttttattttg ataaatcaat atcttgtatt gaatacaatg atgtgacaaa
    26221 aaagatggtg ttagggctga agtataacca aaaaaccttt atggctaagt atatagctca
    26281 aataatgaaa gaaaaattat atttagaaaa cataaaattt gattatatat tatttgttcc
    26341 acttcataaa aaaaggctaa ataaaagagg ttttaatcag gcacaaaaaa tagcatttaa
    26401 tttaagtaaa atagtaaata ttccattatt agattgcata agtagaaaaa aatatacaag
    26461 aatgctatat aaattgaata agaaagaacg aaaagaagaa ttaaaaaatg tatttgttgt
    26521 taaagaaaat gttaaattaa taaataataa aaatatactt ttaatagatg atatatttac
    26581 tactggtcta acaacaaacg aaatttctaa acttttaaag ttatcaggtg ctaataaggt
    26641 ttttgtattg actctactta caaaagcaaa tgataattat attatggaat aatcttatat
    26701 attgacaatt ttagtttaaa atattaaaat gaataagtga tatttaggtc tgtggttgaa
    26761 agtccaagcc agtcgcaggc aaagggatcc acgtaagtta acactaaaaa ttgggttaat
    26821 gatcatggtg cggcttagaa gtaagaccta ccgataataa aatcgagaga gagtagtagt
    26881 gcggcgaacc caagcgaacc ctccagtagg cgagtgtggg gtcaaaaacc aggtcagcta
    26941 aatatcatat taaaagagag ctagcatacc tttatgtata gctctttttt atatataata
    27001 aatttaggtt gagtcataaa gaggtgatat gtttgaataa aagacaagaa aatttattaa
    27061 caacattgct attaaaagaa gagtttataa atgttaataa gattgccaat gattttgagt
    27121 gttcagaaag gacaataaga aatgattgca aatatattga tgaatggtta tctacttttt
    27181 gtaatgctta tatcaaaaga aaacctaata ttgggattaa atttagtgga gatggaacag
    27241 acaaaatact ggtaaatcag aagttgcgag gtgaagttaa aagagtaaat agtgagcttc
    27301 ataagcaagt tgagatatta aatttactac tttgtaataa taaaaaagtt actttaaatg
    27361 atttatctaa taagttgtac ataaataaaa atattgtcag agaagaaata agtaaattat
    27421 cagtagcatt aaataattat aatttaaata tctcgactaa aaaaggcagt ggtatatata
    27481 ttcaagggga tgagaaagac ataagaataa tgttagtatc attcttattt aaatatatag
    27541 ataaatacag attaaacata gatgaaatag agtacttcca tatagtagat ataataattg
    27601 taaaaaacat aatgcttact atagaggagg acatggatat aagacttaca gatatatcat
    27661 tcaaacaatt gatgctattt ttgcttataa atataatacg aataaggctg ggaaattctt
    27721 taaaagtatc aaatactaag aaagagctta agctaaaaat gcttatattg gatatagaag
    27781 atgaattgaa atctaattta tccatagtat taaattatga agaaaaattg tttatagaat
    27841 ctttaatatt gggtatgaac aaacaaataa atagtaataa tattaagaaa tctttatatc
    27901 taaatgaaga attagcaagt tatacaaaaa aaattataaa tttagtatca gaagaatcag
    27961 gaataaattt taataatgat atattacttt atgaacaatt aatatgtcat ttgaatgtta
    28021 caatgcatca aatgaaaagc aatgtttact tagaaaatcc acttttagat aacataaaaa
    28081 ctaaattttg ttttttattt acagtaatat caagttcaat agaaattgaa ttaaaaggaa
    28141 aacaaataac ggaagatgaa attggttatc ttacacttca ctttcaaacc tctctagaaa
    28201 gaaaatacag caaaagggaa tcaaataaaa aggcaagtat tgtatgtcca tttagttttg
    28261 gagtaagtat gctattaaaa gtaaagatag aaaagagatt tgataatata aaaattattg
    28321 aaacattaag agaggaagat ttaaaaagag ataacttcaa taagactata gattttataa
    28381 ttgcatttca agagtatgaa aatatccaaa aaccaatttt tataacaaca ccattattta
    28441 ctgatgagga tgagaataaa cttaaagagt tcacaaacaa aataaaagaa aaagacactt
    28501 catataaact tatgaacaga ttaatgatgt ctgattattt aataagagag ttagaacatg
    28561 atgatattta tgaagctata acatatttaa ctaatttatt aattgaaaaa cattatgcag
    28621 aaaaagagta tttacaatct attataacaa gagaaaagtc atatccaacc aatgttggaa
    28681 aaggaatatt attccctcat ggagacatga aatatataaa acaatctgtt ttatgttttg
    28741 caaggttaaa aacaccaata aaaattagaa atggaaagga tataaagatt atacttttgt
    28801 tagcatataa aaaagatgat gatagaaaag tatttagaga attatttaaa gaaatatcta
    28861 atttaacaga ggatgaaaat atgctagata ttctaagtaa atgtgacatt gaaaaggtaa
    28921 aagatattct tatataaaat tcatagtgaa ctatggataa gcacaaaatt ttccatgtga
    28981 tggaaaaagt tgtgcatgtt tttttgtttt tataagatat tatatgtttg taaggtataa
    29041 ataatctaaa agtagagaag caacttagga ggaaaaaatg gaagaaatag ttttaaagat
    29101 tataattcat gctggaaatg caaaatctat gctttatgaa gcacttgact atgcaaagga
    29161 aaacgatttt aaaaaagcag atgaattaat tgaaaatgca aatgaagaaa ttttaaaagc
    29221 acataaagta caaactgaac ttatacaaaa ggaagctggg ggagataaat cagatatatc
    29281 tattttattg attcactctc aagaccattt aatgacttgt atgagtgaga gaaaccttat
    29341 aaaagaaatg attatgttaa gaaaagaaat tcaaaaatta aaataaaaaa attaaggggg
    29401 atttaaaaaa tgaaaagaaa agtatattta ttttgtagct ttggtatgtc aacaagttta
    29461 cttgcagata aaatgcagaa ggtagcagat gaacacaatt taccaataga agtagaggca
    29521 tttccgatag cagaaattga caaaatagtt gaagaaaaaa atccagattg tatattatta
    29581 ggaccacaag ttaaatacat gctaaaggag ttaaaaccaa agtttgaagc tcaaggaaaa
    29641 cttatagata taatcaatga agttgattat ggaactatga atggggaaaa agtattaaag
    29701 cttgctataa agctaataaa atctaaacaa gcgtagaatt atagtaaaaa ataataggaa
    29761 atgagggtgg atagatgaat agattggaaa gaatacttat gccattggct gaaaaaatag
    29821 gtaaaaataa attattaatt gcaattagag atggtttttt agtatcatca ccattattaa
    29881 taataggttc attattttta ttagtagcaa attttcctat aaaaggatgg aatgaatttg
    29941 taggtcaaat ttttggtcca gattgggctg taaaattgca acaaccaact gtagcaacat
    30001 ttgaagtaat gactctactt gcagttttag ggattggata ttcttatgca aagcaaatag
    30061 atgtagaccc aatagcatca gcagcagttg caatagttgc cttttttata ataacaccat
    30121 ttgtaatacc atatacacca gaaggaacgg aatcagttta catggtaaca ggtataccac
    30181 taggttggat gggttctaag ggaatgtttg taggtatgat aacagcaata acctctgtaa
    30241 aattatttgc tgcagttgta aaaaaaggat ggacattgaa aatgccagat ggagttcctc
    30301 caactgtatc aaaatcattt gcagcattaa taccaagtgc aatagttatg gtagttttct
    30361 ttttagttaa gatagctttt gaagctacac catatggaag tatacatgaa tttatattta
    30421 agtttttaca aatgccatta ttaaaattag gaaatagttt aggtgctatg gcaatcgctt
    30481 atatattctt acatttattc tggttctttg gaataaatgg aagtagtgtt gttggggctg
    30541 tgtataatcc aatattaaaa attctatcag ctgaaaactt agctgctttt caagctggtt
    30601 caaaaatacc aaacataata acaggtcaat tccaagatat gtttgcgaca tttggtggag
    30661 caggtagtac actatcatta gtcatagcta tgttattaat atgtaaaagt aaacgtataa
    30721 agtcaatggg aaaactatca attttaccag gaatatttgg tataaatgag ccattgatat
    30781 ttggtctacc aataatgctt aacccaatac tattgatacc atttgcaata gttccaacaa
    30841 taaatataat aatagcttac ttctgtatgt cagcaggatt agtgcctctt acaaatggag
    30901 ttcagttacc gtggacaact ccaattatat tctctggttt tttaacaaca ggatggcaag
    30961 gtgcagtctt acaattagta cttcttatac taggaatatt tatgtatata ccatttataa
    31021 aaatgctaga taagcaatac ttgagagaag aaaatgaaac tgttgaagag gaagatgatg
    31081 atatatcttt tgatgattta gtattatagg aggaagatta tgaagaaaat agttatgata
    31141 ttggaccaaa tacaagctgg agctggagga aaagagaaaa gtaatatacc tcctgctgga
    31201 aaatcaagcc cattaggacc aggtgtaatg atggagcaat ttttaaatga atctaaggta
    31261 atagctacct tattttgtgg ggatgaattt tttgtaaata atcaagaaga agtaacgagt
    31321 aagatgatag ctatggtaaa aaaattaaac cctgatgtag taatctgtgg accaagcttt
    31381 aattacgaaa acttttctaa gatgtcagca atactgtcta aaaatataaa tgataaaaca
    31441 gatataccag cttttgcagc tatgtcagaa gaaaatatag atgtgataaa cgaatataaa
    31501 aatgacatat gtatagttaa gactccaaaa aaaggtggaa ttggtcttac tgattcgtta
    31561 aataacatat gtaaattggc aaaagcaata gcaaataaag aagatataac tttgatgaaa
    31621 gaagaatttt gttattaata agagtattaa ttattagcaa gtttaaatat tatactattt
    31681 ataataagca tacattagta aaatctatat aatttaagga ggggaatttt aacatgtggg
    31741 gaatgatagc gacatggaga atggccctag aaggaataac tgaggcatca gaacatttag
    31801 aaaaaggcat ggaagcaggt gatgctgtag agcttgctgt aagaagggtt gaagactatc
    31861 cattctataa atcggttgga tatggtggac ttccaaatga aaattgtgag gtagaacttg
    31921 acgctgcatt tatggatggt aacacattag atattggcgc agttgctggt ataagagatt
    31981 attctaatcc tgtcagcata gctaggaagt taagttatga gaaggtaaat aattttttag
    32041 ttggaatagg tgctgaagat tatgctcata aaaatggatt tgaaagaaaa aatatgttga
    32101 ctgatagagc gaagttacat tataaaaaga gaaaaaaaga gactttagac aaaggattaa
    32161 gcccatatgc tggtcatgat actgttggta tgatatcttt agacaaaact ggaaaaatgt
    32221 gtgtagctac atcaacaagt ggattgttta tgaagaaaag aggtagaata ggagattctg
    32281 cactatctgg ttcaggattc tatgttgata gtagtatagg aggagctaca gctactggtt
    32341 tgggagaaga tttaatgaag ggatgtatat cttatgagat agttagactt atgaaagaag
    32401 ggaagcatcc acaagaagca tgtgatattg cagtaaatga acttgataaa cagcttatca
    32461 aaagaagggg aaaagctggt gatttatcat tagttgccat gaataataaa ggtgaatggg
    32521 gagtagctac aaatatagct gattttactt tttcagtagt aactaaagaa gaatctccta
    32581 ctgtatatat ttcaaataga gtagatggaa agactataca taaggttata tcaaatgagt
    32641 ggatggaagc atatgaggct agaataaaag caccaataga agtaagtgag taaagtagtg
    32701 gttacataat taaaatagag ttttataata aggagatttt tatgaatatc ataagtaata
    32761 aaatagatga gttaaaagaa gatttattaa gtgatattat agatattgta aaaataccta
    32821 gtgtcaaagg ggagtcagaa aatggcttcc cttttggcga aaaagtaggg gaggcactta
    32881 ataaagcatt agaaatatca gaaaaattgg gatttaaggt tagaaacttg gataattata
    32941 ttggttatgc agagcatgga gatagtgatg attatgtttg tgtaattgga catgtagatg
    33001 ttgtacatga aggagatggt tggaagcatc agccttataa gggagaagaa actaatggaa
    33061 gaatttatgg acgaggagta ctagacaata aaggacctat aatgtctgcc ttatatggat
    33121 tatatgctat taaagaatta aacttaaaat tagataagag agttagaata atatttggta
    33181 caaatgaaga aagtggattt gaagatatac catattattt agaaaaagaa aaagcaccta
    33241 ttatgggatt tacaccagat tgtaaatatc ctgttgtata tggtgaaaaa ggcatggcaa
    33301 agattagaat aaaaagtaaa atcaattatg aagaagatgt atacctaggt tttatagaaa
    33361 atatgtctga aaatgtgctt gttacatata aagaactgaa tatagaaaat agtgatacta
    33421 tattagatat aaaggtaaaa tacgatttta gttataaatt gaaagatgta ttagatgaaa
    33481 taaaagctag ttttccaaat agtatcgata tagaggtaat ttctaatttt aatcctgtat
    33541 attttgataa agagagtaat cttgttaaaa agcttcagct agcttatgaa agggttacat
    33601 cattagatgg aactccagta acaactaatg gaggtacgta tgcaaaagta atgccaaata
    33661 tagttccgtt tggaccttca tttccaggac aaaaaggaat agctcataat cctgatgaat
    33721 acatggatat agaagatata atattaaatg caaagatatt tgcaaatgca atatatgaat
    33781 tagcaaagga gtagttatgt tagaaataat aggaatgtca gtagaagacg ctaaaattat
    33841 agaagattgt ggtgcagata gaatagagtt agttagtgct ttaactgaag gtggattgac
    33901 accaagtttt ggtcttatag aaagtgttgt taacagtgta aagatacctg taaacgtaat
    33961 gataagacat catgcgaaaa gttttgtata cagtaaagaa gatataagta taatgcaaaa
    34021 agatataagt gtggttaaag agattggtgc taatggagtt gtatttggtg tattggataa
    34081 aaacaacaat atagatgaga aaaatctaaa tgttttattg aaatgttgtg ataatcttga
    34141 tgttacattt catagagcca tagatgaatc aaatacaata gatagtgtta aaattttgaa
    34201 ggattatgat aaaattacga atatattaac atcaggtggg aaaggaagta tagttcataa
    34261 catacaaatg ataaaaaata tgatgcttag ttctaatcac ataaaaattc ttttaggtgg
    34321 aggtcttaat tttaataata tagaaaagat aaaggaatta actaaggcaa gtaattttca
    34381 ttttggtaca gcgattagaa taaataacag tccttttgaa gatatagata gacaaaagct
    34441 taaacaactc gtaaatataa tttcaagata gtatatgaat ataaaatact tagtttaaat
    34501 aaagagagta agttaattat aatttgggta tatgaatgtt aataggtatt aaatatgaaa
    34561 ttattgttga gtatgcttta taacttttaa gtagttttaa atataaataa tgaaagaaaa
    34621 gatagattat ggaggtttta gagggtttat atttattaaa aaattataaa ggtatattgg
    34681 catataatac acataatttc ctaaaattac catcttatga catgaattca catattaata
    34741 aacaattaat ggagattttt gtggatagaa ataggagtaa aaatagcttt aaataaacaa
    34801 aaacaagagt tattaacagt tttatccaca ttatccacag gtgtttgtaa taatattaat
    34861 ccacataagt atcaatgtaa ctaaaaatga taaataattc tatcaattga atagaaaaaa
    34921 tcataaaagt aaataataaa aagaggatta tgagagttcg tgtagaatat attattaaag
    34981 tataaatttt cttatactgg cataattgaa gggagatgta tttatgaaca taattatatc
    35041 tggaaaacaa atcgaattaa ctgatgcaat aaaaaataaa atcgaaacta aactaagtaa
    35101 gttagataat tacattcatc cagacactga tgtaaaagtt acagtaagtg cgagaaaagc
    35161 aagacaaaaa atagaagtaa caatagctac tgtaaatggt cctataatta gagcagaaga
    35221 tatgcaggaa aatttataca cagctataga tgttgtatat gataaattaa gtaaacaatt
    35281 aaaaaaatat aaaaagagat tacaagataa acataaagac aacaagagta taagattcca
    35341 agatgcagag ataaatctag ctgatgaaca agaggattat ggggatgaaa atcaagaatt
    35401 agtaattcaa agacgtaaga aatttagtgt taagccaatg agtgaagaag aagctgtatt
    35461 acagatggaa ttaataggac atgattttta tatgttcaag aacatagatt cagatgaaat
    35521 agcagtagtt tataagcgta ataatggtgg atatggtata atagaacatg aataaagcaa
    35581 tttagattta atcatattag ctatcgataa ataaattatc gatagctttt taaattaata
    35641 ttaaaagtat agtttttatg gtaaaatgaa taatagtaca ataaagaacg aggagagatg
    35701 taaaatatga gctttatgga taatttattc aacatggcag ataaaaaaga attaaaaaag
    35761 ttcaataaaa ctgttgatat aatagattca ttagaaccta aatttgaatc tatggcagac
    35821 tcggaactta aaaatatgac aaatatattt aaagaaagat tagcaaatgg tgaaagtata
    35881 gatgatatac ttccagaagc ttttgcagta gttagagaag tttctaagag agtgttagga
    35941 cttagacact acagagttca aatgattgga ggtatagtac tacatcaagg aagaatagca
    36001 gaaatgaaaa caggtgaagg taaaactttg gttgcaactg ctcctgttta tttgaatgcg
    36061 cttactggaa aaggtgtaca tgttgtaaca gtaaatgatt accttgcaaa acgtgataga
    36121 gaccaaatgg caaaaatcta tgagttttta ggaatgagtg taggcgttat tatacatggt
    36181 caaaatccaa aagttagaaa agaacaatat gattgtgata taacttatgg tacaaataat
    36241 gaatatggat ttgactatct aaaggacaat atggtaatac acaaagagca aagagttcaa
    36301 agaggattaa attatgctat agtggatgag gttgactcta tactaataga tgaggctaga
    36361 acaccactta tcatatctgg acctggagac aaatcaactc acttatattc agatgcaaac
    36421 acatttgttt taaccttaaa accagatgat tatgaattag aagaaaaaga taaggctgta
    36481 tctctaacag catcgggtat acaaaaagca gaagtgtatt ttaatgttga taatataaca
    36541 gatatatctc atacagaatt ataccatcat ataaatcaag ctttaagagc acatgtaata
    36601 atgaagaaag atgttgatta tgttgctaaa gatggagaaa tagtaatagt agatgagttc
    36661 acaggtagac ttatgtttgg tagaagatat tcagaaggtt tacatcaagc aatagaggct
    36721 aaagaaggtc ttaaaataca aagagaatct aaaacactag ctactgtaac attccaaaat
    36781 tactttagaa tgtacaaaaa actttctggt atgactggta cagcaaaaac agaagaagaa
    36841 gagtttaaag caatctacaa aatggatgtt ttccaagtac ctactaataa gcttatgata
    36901 agggaagact taccagattg tgtatacaaa agtgaaatag gtaaatttaa tgctgtagca
    36961 caagaaataa ttgaaaggca caaagtcaat caaccaatac ttgtaggtac agtatcaata
    37021 gaaaaatcag aattattatc acaaatacta aagaaaaagg gcataaagca cgaggtatta
    37081 aatgctaagc atcatgataa agaggcagaa ataatagcac aagctggtag acttggagct
    37141 gttacaatag ctactaatat ggctggtcgt ggtacagata tagttctagg tggaaatcca
    37201 gactttttaa ctaagagaga aatgagaaga aatggattta aagaagaaat agtaaataga
    37261 gtagatactc caatagaagg tatacctgta aaaggtaatg aaattctatt tgaagctaga
    37321 gaagagtatg aaaaactatt tgaaaagttt aagcaacaaa cacaagaaga acaaaaacaa
    37381 gttgtagaag caggtggact tgctataatc ggtacagaaa gacacgaatc tcgaagaata
    37441 gataaccagt tgagaggtcg tgctggacgt caaggtgacc cagggagttc aagattctat
    37501 ataggtcttg atgatgacct tatgagattg tttggtagtg atagaatatc tggtattgta
    37561 gataaaatag gtcttgaaga agacatgccg atagaacata gaatattatc taagtctatt
    37621 gaaggagcac aaaagaaagt cgaaggtaaa aactttggaa taagaaagca tgtacttcaa
    37681 tatgatgatg ttatgaataa acaaagagaa ataatatatg cagagagaaa acgtgtatta
    37741 gaaggtgaag atttacaaga acaaatacaa tcaatgactc attctataat agaagaagca
    37801 gttactcttt atacacaaga taaaggattt gatgaagaag gattcaaaga acatatgtat
    37861 aatctgttct tgccaaaagg tagcatagaa ataccagaaa tagaaaaact taatccagtt
    37921 gaaataacag aaaaagttta tgaaatagct atgaagatat atacttcaaa agaagaacaa
    37981 gttggctatg agagaatgag agaagttgag agagtaatct tacttcaagc tgttgataac
    38041 cattggatag accatataga tgctatggac caattacgcc aaggtattgg tcttcgtgct
    38101 gtaggtcagc aagacccagt tattgcttat aaaatggaag gttttgatat gtttgatgaa
    38161 atgaataaac atatcaaaga agatactgta agatatttat ttaatataac aatagaaact
    38221 ccagtggaaa gaaaagcagt tgtagatgtt gaaaatctat cttctccatc agatggaaca
    38281 cttccaacaa gtaaaactgt taaaaaagat gaaaaggttg gaagaaatga cttatgccct
    38341 tgtggaagtg gtaaaaaata taaaaactgt tgtggaagat aatctcggag gattaaatgt
    38401 taaagttaca agagtataga catagtatgg agcagatggc ttcaaattta gaagaattga
    38461 gggtttctct ttgacatagc ttcattgatg aaaaatatac aaattaatga agaaaagatg
    38521 aatcaacaag acttttggaa tgataatgaa gtcgctcaaa gagtacttca agaaaataaa
    38581 tctttaaaag agacattaga agaatatgaa agcttaaaaa gcttattaga agatatagaa
    38641 gtattaattg aaataggttt agaagaagat gatgattctg tagaaagaga tatagaaaag
    38701 tccatagaat caatggaaga aaaattatct gaaatgaaaa taaagactct tctaaatgga
    38761 gagtatgata aaaataatgc tatattatct ataaatgctg gtactggtgg cttagatgct
    38821 caagattggg ctcaaatgct tttgagaatg tatataagat ggtcagaaag taagggttat
    38881 aaagttaagc ttttagatat aatatcagac cctgaagcag ggataaagac agctacaatt
    38941 cttgtagaag gaacaaatgc ttatgggtat ttaaagagtg aaaaaggagt tcatagatta
    39001 gtgagaatat ctccatttga cccatcagga aaaagacata cttcatttgc atctatagat
    39061 gtaacaccag agttagatga aaatatagaa gtagaaatta atccatcaga tttaaagata
    39121 gacacatata gagcttctgg agctggaggg caacatgtaa atacgactga ttcagcagtt
    39181 agaataacac atatccctac tggtgtagta gttcagtgcc aaaatgaaag gtctcagcat
    39241 ctaaataagg acagagctat gagactttta atggcgaaat tgatagaatt aaaagagtta
    39301 gagcaaaagg aaaaaataga agatatacaa ggcaaatact cacaaataac ttggggtagt
    39361 caaattaggt catatgtttt tcaaccatat aaattggtta aagaccatag aacaaatgct
    39421 gagtttggta atgtagatag tgtaatgaat ggaaatatag acttgtttat taatgaatac
    39481 ttaaaaatga ataaaatagt ttaataaata tgcactgtaa ttaagatata tgtataagaa
    39541 ggttagtgct taatatgcac ttacagcact taggctgagg acgataaccc tcagccttcg
    39601 ttctgttttg ttatacttat gaaaaactaa cattaggagt gaataaattg gatataaatc
    39661 aaatcttaaa aaaagagttt aatcttagag atgaacaaat aaataataca ttaaaactta
    39721 ttgatgaagg aaatacaata ccatttatag ctagatatag aaaagaaatg actggcgaaa
    39781 tgagtgatgt tacactaaga gaattttatg aaaaattaat gtatttgaga aatcttcaaa
    39841 gtagaaaaga tgatgtagtt agactaatag atgaacaagg gaaattaaca gatgaaataa
    39901 cacaaaatat agaaaaagca aaaacattac aagaagtaga agatatatat gctccatata
    39961 aacaaaagaa aagaactaga gcaacaatag caaaagaaaa aggattagaa aatttagcat
    40021 tgtcaatact agagaataat ttagataata tagaaataga ggcaaagaat tatcttgatg
    40081 aagagaaaga agttctttct attgaagatg ctctaaaagg tgctagagat ataattgcag
    40141 aactagtctc agatgatgct aagattagaa agtatataag agaattagct ttaagagaag
    40201 gtatgatagt tagcaaaagt gctactgatg agaaatcggt ttatgatatg tattatgatt
    40261 atagcgaagc agtaaaaagt atggctccac atagagtttt ggcaataaac agaggagaaa
    40321 aagaaagctt cctaaaggtt aagttggaaa taaataatga taaagtttta aattacataa
    40381 taaacgaata tgtaaatgat aaaaatttca aaaataaaga agaaatcgta agttcaatag
    40441 aagattctta taaaagatta attttcccat caatagaaag agaaataaga aatcatttaa
    40501 cagaaatagc tcaagaaaga gctataagtg tatttggaaa gaatgttaaa agtttattgc
    40561 ttcaaccacc agttaaagac aaagtagtta tgggatttga ccctgcattt agaactggat
    40621 gtaaaatagc tgtagtagat aaaaatggta aactactaga ttatacaaca gtatatccaa
    40681 cagaccctca aaatgatgta gagggagcaa agaaagtttt aaaaggattg attgaaaaat
    40741 acgatataga tataatttca attggaaatg gtactgcatc tagagaatca gaaacttttg
    40801 tatcagaaat gataaaagaa atagatagcg aagtgcagta tgtgatagtt agtgaagcag
    40861 gagcatctgt ttattcagca tctgaacttg caaatgagga acatccagat ataaatgtat
    40921 caataagagg agctatatct atagctagaa gattacaaga cccattagca gagcttgtaa
    40981 aaatagaccc taaaagtatt ggtgttggtc aatatcagca tgatttaaac aaaaagagat
    41041 tagaagaagt actagatgga gttgttgaag attctgtaaa tagtgttgga gtagatttaa
    41101 atactgcatc atactcccta ctagaacatg ttgcaggaat aagtaaagct atagcaaaaa
    41161 atataatagc atatagagaa gaaaatggag attttacgtc aagagcacaa ttgaaaaaag
    41221 taaaaagact aggacctcaa gcatttactc aatgtgctgg atttatgaga atcttagagg
    41281 gtaaaaatcc actagataat acaggtgtgc atcctgaaag ttatgatata tgtaaaaaaa
    41341 tgattgagat aattggatat tctttagatg atgtaaagaa taagaatata ggtgaaatag
    41401 atgaaaaaat aaaagaaatt ggcttgaggg aattaagtga aaaactggaa gtaggacaag
    41461 ttacattaaa agatattata gctgaaatca aaaaaccagg aagagaccca agagaagaag
    41521 gtataaaacc tatacttaga actgatgtct taaaaattga agatatacaa gagggtatga
    41581 ctttaaaagg tacaataaga aatgttgtag actttggtgc atttgtagat attggaataa
    41641 aaaatgacgg attagttcat aaatcagaaa tgagtaatag ttttgtgaaa gaccctatga
    41701 gtattgttac agtaggtgat atagtagatg taaaagtcat tggaattgac ttaaacaaaa
    41761 aaagagttgc cttatcaatg aaaaaataga taagtttatg tatggttata gtacttagat
    41821 tctatatttg taaatgagat tactcgagtg tataaatgtt aaaaatttgt aaaagtatat
    41881 agtaagaaaa tatattaaaa taagaacgat agtcttgaaa atcaggagga aaatgaatga
    41941 tttttataaa aaatggtaaa ataaatacca taacaaatgg gataattcat ggagatatac
    42001 ttatagatga aggaaaaata atagaaattg gagaagattt gatagctcct ttagatgtag
    42061 aggtaataga tgctagtaat aaacttgtgt ttcctggatt tatagatgct catactcact
    42121 taggcttatg ggaagatggt atagggtttg aaggagcaga tggaaatgaa gagactgacc
    42181 caataacacc acaattaaat cctatagatg gaattaatcc aatggataga acttttaaag
    42241 aagcttttga aggtggaata acttctgttt gtacaacccc tggaagtgca aatgttatgg
    42301 gtggtcaatg tatagctata aagacatgtg gaaaaagaat tgataaaatg gtaataaaaa
    42361 atccagttgc atctaaaatt gcttttggag aaaatccaaa gagctgttat ggtcaggatg
    42421 ataaatctcc tcaaactaga atggctatag ctgctttact tagagaaaac ctaaaaaaag
    42481 cagaagaata tttagaggat attgatatgt atgagagtca tgatgatgaa gactgtgaaa
    42541 aacctgaata tgatataaaa atggaaagtt taataccagt tttaagaaga gaaattcctt
    42601 ttaaggcaca tgctcataga gcagatgata tgtttacagc aataagaatt gcaaaagaat
    42661 ttaatttaaa gcttacatta gaccattgta cagaaggtca tcttattgta gatgaattag
    42721 tggaagaaga gtttcctgta atagtagggc catctttgtc agaaaggtct aaatttgagc
    42781 ttagaaattt gacatttaat acagcaggaa tactttcaaa tgcaggtctt gatgtatgta
    42841 taatgacaga ccatccagta ataccagttc aatatcttcc aatatgtgca ggtattgcag
    42901 taaaacatgg tatgaaagaa gaaaaagcaa tagaatcaat tacgataaat ccagctaagg
    42961 ctttaggaat agaagataga gtaggctcta tagaagttgg taaagatgca gacctagtaa
    43021 tttgggataa ttctccacta gagattcaaa gtaatgtact ttacactata ataaatggca
    43081 aagtagtgta tgagaaaaaa taacttgaca attattaaaa aattaactaa tataagagtg
    43141 taaggaaaat taaaaaatca aagaagatct tcggggcagg gtgaaattcc ctaccggcgg
    43201 tatagcccgc gaactactta ggtggttgat ttggtgagat tccaaagccg acagtaaagt
    43261 ctggatgaga gaagatattt atggaagata aataagttta ttttagtttg tttaaactat
    43321 atccataaaa actcgaagat agggtcttcg agttttttgt ttttcctaaa aaatataaac
    43381 atattgatta gggggaaaat aattatgcaa aacacagcaa aaagaggcaa tatgatttca
    43441 acaagaactt tagtagtaat gtcaatttta tctgcaatat cttatttatt aatgtttatt
    43501 caagcaccaa taccaggtat atttccagat ttcttaaaaa ttgacttatc agatattcca
    43561 gcaatattcg gaggaatgtc tctaggacca ttagttggat ttggaatagt tgtagtaaaa
    43621 aatgtgttac aagcaataac agcaacaaca actggaggaa taggagaatt tgcaaatata
    43681 ttaattggtg gttcttatgt aatgatactt tgttattcat ataagagaag tggagattta
    43741 aaaggtgtac tgattggttt tgtattagga acaatatcta tgactataat tggttcatta
    43801 gtaaattatt ttgtaatgtt gccactttat ggtcaactta tgggactaga tgctataata
    43861 ggtttaggtt cagctataaa tcctcaagtt catgatttat tcacttttgt aatttggatg
    43921 atagctccat ttaatgtgct gaaggctgtt ataatatctt tagtaacatt gccactttat
    43981 aaaaaaatgg gaaatataat aaagaaataa ttagacaaga aaatataaaa ttaaatgtat
    44041 tgaatagcca taatttattt ataacttcag tcctatattg tgattgatat attaaatata
    44101 ttatggcttt attaatttat caaaaaatgt atataaaata agaaatattt agacaaattt
    44161 aaattttaaa ataaaggaag aattaaaacg attaactatt aaaatatgaa aaaggtatct
    44221 acaatataca gagcgaattt ataataaaca aaaaataaag ttattattaa aaaactataa
    44281 agattaataa tcaaactttt aataataact aatttaataa tgataggggt gtatattcat
    44341 gcatttatct aaaaaaataa aaaaattatt tttagatgat aaagggtatt attttaatag
    44401 taattggaaa gatattcata ttggtattta tgatacaaaa ttaggagatt ctttaaagta
    44461 tatcactaat gaatatattg aaaatatgga aagggtaaaa tacgattatc gtgaattaaa
    44521 aaatatagaa aatcaaactg atggtatgtg tattttagca ataaggcaga attacaaggc
    44581 attagagtat gtaaaaaatc aaaccgaaga attatgttta gaagcaataa agtataatta
    44641 taaagcactt gaatatgtaa aagaacaaac agaatacttg tgtcttgaag ctataaaaaa
    44701 agattgtaat gcacttaagt atgtacgaaa taaaacagaa gggcttatta taaaagcaat
    44761 aagtcattct tccaatatag atgttgtaag tatactaaag gctttagaga ctcaaacaag
    44821 aaggatatgt ttagaagcaa ttaaaaaaga tggcaggtgt cttgcatacg ttagagaaca
    44881 gagtgaagaa ttatgtatag aggccattaa gcaaaattat aaagcattaa aatatgtaaa
    44941 aaatcaaacg gaaaaaatgt gtatagaatc cgtaagacaa aatggtatgg cattacaata
    45001 tgtaaataaa caaactgata aaatatgtat agaagctgtc aaacaagatg gaagaagtat
    45061 acaatttgtc aacaataaga cagaagaaat atgtataaat gctataagat atttaaataa
    45121 aaaatataat attaaagatg tactaagtta catagataag tatacagaag atatatgtat
    45181 agagattgta agacaaaatg gaaaaatgct tatgtatata aaaaatcaaa cagaaaaaat
    45241 gtgtattgaa gcagtaaaag agaactataa gtctcttaag tatgtaaaag agcaatctga
    45301 gagaatatgt aaagaagcat tgaagcaaaa ccataaagca aaagagtatg taaaaattgc
    45361 tattgatgat tgtatataag gtgtagatac agccatcaat agtgaaagat aaaaaaagat
    45421 aaagacaagt actttataat aatctaggct tatactataa ttaattaagc agtgatataa
    45481 attcacatga cgattatatc aaaatggaga tgaattgatg gcaaaaatat atttagaaaa
    45541 tgaaaataaa actagagaaa taggatataa attaggtaaa cttttaaaag aaggcagtgt
    45601 tatctgttta gtaggagatt taggagctgg taaaactaca atgacacaaa gtttggcaga
    45661 ttcattaggg atagaagatt atataacaag tcctactttt actataataa atgaatatga
    45721 aggaaaaata cctttgtatc attttgatgt ttatagaata ggtagtagtg atgaaatgta
    45781 tgatattggt tatgatgaat atgtaaattc aaatggaatc tgtataatag aatgggctaa
    45841 tctaatagaa gatatacttc caaaagaata tttgaatata gaattgagat ataaagatga
    45901 aggaagagaa atgatattaa ctccaaaagg agagttttat aaagagatag ttgaggagtt
    45961 gattaaataa tgaaaatact aggtatggat acgtcttcta tggcagcaag tgtagcagtt
    46021 gttgaagatg ataacttaat atgtgagttt acagtaaaca acaaaaaaac tcattcacaa
    46081 aagcttatgc caatgataga aaatatgttg agtatgagtg atttaagtat aaaagatatg
    46141 gatttattag ctgtatgtat aggtccaggt tcgtttacag gacttagaat aggtatggct
    46201 acagtaaaag ctatggccca tgtaaataat ataccaataa tagcagtaaa ttctttggaa
    46261 tctctagcaa ataacataaa tttctgtgat agaaagatat gttgtatatt agatgcacag
    46321 aaaaatcaag tttattcagc taaatacaag tttgaaaatg gagagatgat agaattagat
    46381 ggtgttgatg ttgtagagtt tgatgcttta gtagatgaaa ttgtatcaac taatgaagaa
    46441 tttataatag ttggagaagc tgtatacaaa tataaagaca agttagagaa tataaaaaat
    46501 ataaaaatac cttcaccagc taataatgta tctaacgcag gaagcttatg ctctttagca
    46561 ttaaataaat ataataaaaa tatagatgta catacttgtt atacgataaa tcctatgtat
    46621 atcagaaaat ctcaagctga agtacaatat gatgaaaaaa tgaagaggct aaataatgga
    46681 agataataca aaactaatag tagatgatat aaaaatagaa gaaatgacta cagaagatat
    46741 agatgaagtt tttgaagtag aaaaaaattg ttttgaagat tattggtcaa aagagtcttt
    46801 tagaaaagag ttgtctaatg aagtagcaag gtatatagta gctaagttaa atggaaaaat
    46861 tgtcggatat gtgggaattt ggcttatact tgatgaaggg catataaata atgtggctgt
    46921 tcatagtgac tatagaggga aaaaaatagg ggacaagttg ataaaaggca tagttgattt
    46981 atgtaaagat aataatattg catctatgac attagaagtt agagcatcaa ataaaatagc
    47041 acaaaattta tatagaaaat atggatttaa aatgggtgga ataagaaaag aatactacag
    47101 tgataataaa gaagatgcaa ttataatgtg gaaccaatta aaagaggtgt agagtttatg
    47161 agtgatataa taacattagc tatagagagt agttgtgacg aaacagctgc atcagtttta
    47221 aaaaatggta gagaaatact ttcaaacata atctctactc aaatcgagac acataaaaaa
    47281 tttggtggtg tagttccaga agtggcctca agaaaacatg tggaaaatat tgatatagtt
    47341 gttcaagaag ctttagacaa agcaaatata ggttttaatg atatagacca tatcgcagtt
    47401 acatatggac ctgggctagt aggtgcttta ttggttggtc tatcttatgc taaagcactt
    47461 gcatatactt tgaatattcc acttgtagga gtaaatcata tagaagggca tctaagtgca
    47521 aactatatag agcataagga tttaaaacct ccttttataa cattaatagt atctggaggt
    47581 catactcact tggttgaagt aaaagattat ggcaagtatg aaattttagg aaaaacaaga
    47641 gatgatgcat ctggagaagc atttgataag atttcaagag ctatgaattt aggctatcca
    47701 ggaggtccta ttattgataa tctggccaag aatggaaata aacatgctat agaatttcca
    47761 agagcctatt tagaagaaga tagctatgat tttagtttta gtggattaaa atcttctgta
    47821 ttaaattatc taaatggaaa aagaatgaaa aatgaagaaa tagtagttga agatgtagca
    47881 gcatctttcc aagaggctgt agtagaggtt ttatctacta aagcactaaa agcagtaaaa
    47941 gacaaaggat acaatatcat aactctttct ggtggggttg cttctaattc ggggcttaga
    48001 gctaaaataa cagagcttgc taaagacaat ggtattactg ttaagtatcc tcctctaatt
    48061 ttatgcacag ataatgcagc tatgataggg tgtgcaggat attataattt tataaatggg
    48121 aaaacgcatg atatgagttt aaatgcagtt ccaaatttaa agataaatca gtagagttag
    48181 atgaatttgt aaatataaaa gtattgtttc aaaaaagtaa gtagaagtga aattaaatat
    48241 aataaaaata aatatagtat atattaaaac ttgaagtaaa actatttata atctttatat
    48301 tccaatgaat tgcaacaaat tttaggatat acagattgtc tttggcttag atttgcttca
    48361 agtttatttt tttctataga aagattattt taaaaagtgg gtaatatacc cttttttttc
    48421 attcttaaaa agttttatac taattataga aagattaagg aggggaagaa atgagtcaaa
    48481 gtaaagaaga caaaataaga agtattttgg aagcaaaaaa tattaaatca aattttcaaa
    48541 ataaagaaaa tttaagtgaa tttaatgaaa aaaaagcaag taaaagagca gaagacttat
    48601 tagatgtata ttataataca ttatctactg ctgatatgga atttccatat tggtataata
    48661 gagaatatag aaaatcagat ggtgatatac cagtagttag aagagctaaa gctcttaaag
    48721 ctgctttttc acatatgaca ccaaatataa tacctggtga gaaaatagta atgcaaaaga
    48781 cacgccatta tagaggttct tttccaatgc catgggtaag tgaaagcttc tttgttgctc
    48841 aaggagaaca aatgagagaa gaggcaaaga aattagccag taacacagct gatgaactta
    48901 caaaatttgg gtctggtgga ggaaatgtta ctgaaagttt tggaaatgtc gtttcaatag
    48961 ctggtaaatt cggaatgaga aaagaagaag tacctgtact tgtaaaaatg gcaaaggaat
    49021 gggttggaaa gtctgttgaa gatttaggtt ttcactatga aaaaatgatg ccagattatg
    49081 atttaaaaga aaacctaatg agtacactta tatgcatgtt tgattcaggg tatacacttc
    49141 ctcaaggtag agaagttata aattattttt atcctttaaa ttatggatta gatggaatta
    49201 tagaaatggc aaaagaatgt aaaaaggcag ttgcaggaaa tgcatctgga gatgggctta
    49261 ttggtatgga ccgtctatat ttttatgagg ctgttattca agttattgaa ggattacaaa
    49321 cttggatttt aaattatgca aaacatgcaa aatatttaga aagtatagag acagatttag
    49381 aagctaaaaa agaatactca gatttagtgg agatattaga acatattgca cataaacaac
    49441 ctcgtacatt tagggaagct cttcagttga cttatacaat tcatatagca tcagtaaatg
    49501 aggatgctat atctggtatg tctataggac gttttggtca aattttatat ccttggtatg
    49561 aacaagatat agaaaaagga ctaattacaa aagaagaggt tatcgaatta ttagagcttt
    49621 atagaataaa aatcacttgt attgactgtt ttgcttcagc gggcgtaaat ggaggagtac
    49681 tatcaggcaa tacatttaat actttatcta taggtggatt aaaagaagat ggttctacag
    49741 gagctaatga actagaagag ttattattag aagcaagtat gagatgtaga actcctcaac
    49801 ctagtttaac tatgctatat gatgaaaagc ttccagaaga tttcttaatg aaagctgctg
    49861 aatgtacaaa gcttggttct ggttatccag catgggtaaa caactcaaat ggaacaacat
    49921 ttatgatgaa acaatttgct gatgaaggta tgactgttga agaagcaaga gcatttgcat
    49981 taggtggatg tttggaaacg tctccagggt gttggaaaca gttaacttta aacggaaaaa
    50041 catactctat tgcaggtgga gcaggtcaat ctgctggctc tggagttcac tttatagcta
    50101 atccaaaaat tttagaactt gttcttatga atggtaaaga ctatcgtatg aatatacaag
    50161 tatttgagcc tcataataag ccattagata cttatgaaga agtcatagaa gtatttaaag
    50221 attattataa acaagcaata aatgttttag aaagagcaaa taacattgag ttagatatat
    50281 ggagaaaatt tgatacttct ataataaatt cactactaaa gccagactgt ttagacaaag
    50341 gtcaacatat aggaaatatg ggatatcgtt ataatgcaac attaaacgta gaaacttgtg
    50401 gtactgttac tatggtcaat tcatttgcag cacttaagaa acttgtatat gatgacaaag
    50461 catttacaat agaggaaatg aaggatgcca tattaaataa ttttggattt aaagatgcat
    50521 tagaagtagg aaattattct atggcagacc aagtaaaagt agataagact ggtaaatatg
    50581 atgctattta taaggcctgt cttgatgcac ctaaatatgg aaataatgat ttgtatgcag
    50641 ataatattct taaaaactat gaggtatggt tatcaaaagt atgtgaagaa gcacaatctt
    50701 tatatgctaa gaagatgtac ccttgccaaa tatctgtttc tacacatggg ccacagggag
    50761 ctgctacctt ggctactcca gatggtagac taagtggaac aacatattca gatggttcag
    50821 tctctgcata tgcaggaaca gataaaaatg gagtatatgc attatttgaa tctgcaacta
    50881 tttgggacca agcagtagtt caaaattctc agatgaattt aaaacttcat ccaactacta
    50941 taaaaggaca acaaggaact aaaaaattat tagatttaac aagaagttat ttaagaaaag
    51001 gtgggttcca tattcaatat aatgttgtag attcagaaac attaaaagat gcacaaaaaa
    51061 atcctgacaa ttatcgtcaa ttaatggtac gtgttgctgg gtttactcaa tattggtgtg
    51121 aattaggtaa accaatacaa gatgaagtaa ttgctagaac agagtatgaa ggggtgtaaa
    51181 atatgagaaa gcatagtgat tgtatgaatt tttgtgcagt agatgcaacc aaaggaattt
    51241 gtagattatc aaaacaaatg attaatttag atgatgcagc atgtccagag ataaaagtaa
    51301 tgccaaaatg taaaaattgt aaaaattttg ttgaagctaa tgatgaaggt ataggcaaat
    51361 gtgttggcct agagaaagaa gactgggtat attcaacatt gaatgcaatt acttgtgaag
    51421 gacatgtgtt taatgagtag tcaaaagcaa ttagaaggca tgatttttga tgtacaaagc
    51481 ttttcagttc atgatggtcc aggttgtaga acaactgtat ttttaaatgg atgtccactg
    51541 agttgtaaat ggtgtgcaaa tccagaaagt tggactgtaa gaccacatat gatgtttagt
    51601 gaattatctt gtcaatatga aaatggatgt actgtatgtc atggtaagtg taaaaatggt
    51661 gcattgagct ttaatcttga taataagcca gttatagatt ggaacatctg taaggactgt
    51721 gaaagttttg aatgcgtcaa ctcatgttat tataatgcgt ttaaattatg tgcaaaacct
    51781 tacactgtag atgaacttgt gcaggtaatt aaacgtgatt ctaacaattg gagaagtaat
    51841 ggaggagtaa catttagtgg aggtgagcca ctacttcagc atgaattttt acatgaagta
    51901 ttattaaagt gtcatgaggt aaatatacac actgccattg aaacgagtgc atgtgtatca
    51961 aatgaagttt ttaataaaat atttaacgat attgattttg catttattga tataaagcat
    52021 atggatagag aaaaacacaa agaacaaaca ggtgtgtata atgatttaat attagaaaat
    52081 atatcaaacc ttgcaaattc tgattggaat ggaagattag tacttagagt accagttatc
    52141 tctgggttta atgatagtgt tgaaaatata agtgatatta tatcttttat gcacaaaaat
    52201 aatttggtag aaattaatct attaccattt cacagattag gagaatcaaa atggattcaa
    52261 ttaggcaagg aatatgagta ttctgataag ggtgatgttg atgaaggtca tcttgaagaa
    52321 ttacaagata tatttttgga taatggtata gcttgttatg ttggtcatga gacagctttc
    52381 taaaattaaa tacaagtttt aattaaaaaa aggatgttac actaaaagct agagttacat
    52441 ccttttttat gtggttttta tgtagaaata attaagtaaa gttttatatg ttttatatat
    52501 tgtttatatt gctttatgtc cataaatatt gctttaaata tactttatat attttttata
    52561 gtttaaatta cagttagaga atatattacg cttacttcat tgaagcagaa gttttgtggt
    52621 aaaatactac tatgaaaaaa tttaatagaa tatattttag aaacaactaa aaataatagt
    52681 taggaggaag acatctatat gaacaaatca tggagcaact tgattattaa gaataaagac
    52741 aaaaaagtac gttcagggtg gattattata gctgttatgg ctacctttta catacttcag
    52801 tattatttaa gcatgatact tgtggaaata ataagaaaaa tcctggttgc tacaggtgac
    52861 attaatttaa aaacaaacta tttttcagaa tatgctaatt ggttaaatga tgttgggtta
    52921 cagattgtat ttcaaattct aacagattca cttatgatta ttattcctat aattgtatgg
    52981 agatttatta tgaaaaatcc tattagtgaa atgggattac attcttatag aattaagaaa
    53041 aaagaaggat atgtaggaat gttattagga atatttaatt gtacagtaat ctttattata
    53101 acaataacaa ttggtggtgg tcatgttact tcatgggtac caaaaatttc acctttaaca
    53161 gtttcttgga tacttgtctt tattttagtt gcttttgcag aagaaatttt aaatcgtggt
    53221 ttttttatgg cagttttacg aagatgtaaa aatatttatt ttataatgat tgttccatct
    53281 gttatttttg ggttgattca tatatggaat cctgatgtta cttttttatc tgtaattaat
    53341 attataatta ttgggattct tttttcatat atgtttataa aatcgtcgaa tatatggatg
    53401 tgtataggtt atcattttac atggaatgta tttcagggga ttatatatgg tatgcctgta
    53461 agtggacttc aggttccagg gattataact acacaaatca ctcatggtaa tctattaaat
    53521 ggaggaatgt ttggtattga aggtggaatt ttgactactt ttgttaatct acttagcttt
    53581 atatttgtat ggtattacta tagagattct aagtatgatt ttgttagtaa taagtaagtg
    53641 tgaataatgt taagaactaa ttaattataa taaattttaa taatatattc taggcttaaa
    53701 gtattctgta ggattgttgg aaatttacaa aaatataaaa ataacttgga cattctattt
    53761 actccaagtt atttttatat tcttttgtaa atagaaatgg aacataaaaa attaaaacaa
    53821 ccatagatac tattagtata tcatggtaag tgatagttga ttctttagaa tttgtgataa
    53881 gaaaagcact attatttaaa acatgaatta tagcacaacc agatattctt agtttttata
    53941 taaagatatc ctaaaaatat tgaaattcca actatatgta ttgaacgtaa tattattcct
    54001 aatattggcg cttcaggtgt atataatgta aatattagtg gtaagtgcca caagctccaa
    54061 cacataccta atattataac tcctaacttt ttaccaaatt tatcaaaaaa aatattctgt
    54121 aaaaaacctc tccaggcata ttcttcacta aaaaaaatat aaatagtagt taatgatgtt
    54181 attggagtaa atataaattt tatgatacca ctataattag gttgagcccc ttgtagtatt
    54241 agtataaatg atgttggaac aattacagaa atcataaagt aaataattaa taaagaacct
    54301 agtttaatat tttttacagg atataaattt tttttatttg atgaatatat tataattgat
    54361 aagctactta taaaagttaa t
//
LOCUS       DAGSWI010000007        53467 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.007, whole genome shotgun sequence.
ACCESSION   DAGSWI010000007 DAGSWI010000000
VERSION     DAGSWI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.007"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(16..1713)
                     /locus_tag="MJF80_000362"
     CDS             complement(16..1713)
                     /locus_tag="MJF80_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBZ0279043.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            complement(1779..2369)
                     /locus_tag="MJF80_000363"
     CDS             complement(1779..2369)
                     /locus_tag="MJF80_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBZ0279044.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEILNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            complement(2479..3276)
                     /locus_tag="MJF80_000364"
     CDS             complement(2479..3276)
                     /locus_tag="MJF80_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBZ0279045.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            3632..4153
                     /locus_tag="MJF80_000365"
     CDS             3632..4153
                     /locus_tag="MJF80_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0279046.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHEQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            complement(4573..5787)
                     /locus_tag="MJF80_000366"
     CDS             complement(4573..5787)
                     /locus_tag="MJF80_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0279047.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLNQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHENLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(6252..7187)
                     /locus_tag="MJF80_000367"
     CDS             complement(6252..7187)
                     /locus_tag="MJF80_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBZ0279048.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            7475..8041
                     /locus_tag="MJF80_000368"
     CDS             7475..8041
                     /locus_tag="MJF80_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBZ0279049.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            complement(8232..10268)
                     /locus_tag="MJF80_000369"
     CDS             complement(8232..10268)
                     /locus_tag="MJF80_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBZ0279050.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(10437..11114)
                     /locus_tag="MJF80_000370"
     CDS             complement(10437..11114)
                     /locus_tag="MJF80_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBZ0279051.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            11839..12024
                     /locus_tag="MJF80_000371"
     CDS             11839..12024
                     /locus_tag="MJF80_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBZ0279052.1"
                     /translation="MILLEQRKLRVAFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(12070..12876)
                     /locus_tag="MJF80_000372"
     CDS             complement(12070..12876)
                     /locus_tag="MJF80_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBZ0279053.1"
                     /translation="MKVVIVPGHTLTGKGTGAVGYIDEGKENRILTDLIVKWLKQGGA
                     IVYTGKVDKSNNYLAEQCEIANRQNVDLAVQIHFNANKTTLNPMGTETIYKTNNGKVY
                     AERVNKKLSTIFKNRGAKSDVRDLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKTIDNKENGEGKIMYKHTIVYDGAVDKIPATVVGWGYNDGKILICDIK
                     DYIPGQTENLYVVGGGACEKIGSMTKENYTMIKGNDRFDTLYKALDFINK"
     gene            complement(12876..13142)
                     /locus_tag="MJF80_000373"
     CDS             complement(12876..13142)
                     /locus_tag="MJF80_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279054.1"
                     /translation="MEQLLTELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVEMGAMKTDVEEIKEDVTDIKVMLQKEGDK"
     gene            complement(13189..13359)
                     /locus_tag="MJF80_000374"
     CDS             complement(13189..13359)
                     /locus_tag="MJF80_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279055.1"
                     /translation="MYNILKRMIEQKNYETKEELQTKLDVFYAMNRIKESEYTELTNL
                     LNKEDTPIEPII"
     gene            complement(13385..13702)
                     /locus_tag="MJF80_000375"
     CDS             complement(13385..13702)
                     /locus_tag="MJF80_000375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279056.1"
                     /translation="MNKIKFRIIREHNFSKEEIDNIGIDNFFIADNGQLWTPLISKGE
                     IIKTGEQSYNEWLELQINPVKPEQSLDRGTILEENQKEQDNLLIDNAYRIAILELNTN
                     NVL"
     gene            complement(13705..16035)
                     /locus_tag="MJF80_000376"
     CDS             complement(13705..16035)
                     /locus_tag="MJF80_000376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279057.1"
                     /translation="MLNEDVVKNINKLNNEQDNINLELSKKANKEDLEKLIQGGPNVS
                     VSKDISINNWILEENVYTAIVEHNLVTRKVIVSLIDKSTNNNVFCSYQILDDNSIKVF
                     NELNNELECIVVNGNSSINMISATIDDNRSTETTTYSSVKIEALLKVLENKIDDIENL
                     KEIDNIKCSTDTGEYIIENSKKGYLTNFNIEGKTLIDLWGKTSSDFSLWKATFVDGKI
                     NILTENDIRYSNFFTINYTSYKPDTIYTIIVDVDKNTLPSTSGIYIHSLGEENSVFIP
                     NLTNIAIPGGVIGKFKYTFTTISDLSDCNAVLRSVLDNDTLTSGYEVSLKITILEGDY
                     TDINIDYSNELLSVGQRDKIEFLSYQYSGINIFNKNADFKDNYILQYLNGEELISEAS
                     NHKYTLDYIEIEPDTEYTFYNCSRNICWYDINKSFIPAPLNERIIGDKDIFYVARSPK
                     NAKYLRVTIIKDLHDNGNKTIITKGNKYDKKTIPHTLRGLANGIRDEIVYKNGSYKLI
                     KRCEEIVFNGNENWIEVHGMYEFRLNLDISVLRLDMSKFITNLYKQSHVDYIFNVQTE
                     SALSISTENGIGVIDIVDTSISPFSIANFKEKLKKNPLKIIYELEIPREVELGLLNLE
                     QYDNQTGFICSSGITIPNVSFESTRNLGSHIEVIRENLKDITTLTESNVDFKFINGWD
                     KVWQSATITKVGKYLFVNMIVTKSTSSTVLEEAIIKLPLGISRQNYSCPVMNSSCEQV
                     DTVGIACVNNTILMLTPGHDITDKGILISVIVNLEV"
     gene            complement(16070..17860)
                     /locus_tag="MJF80_000377"
     CDS             complement(16070..17860)
                     /locus_tag="MJF80_000377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBZ0279058.1"
                     /translation="MSWAEVYKINSDIQGEPLNFLNYLQDLKLNGSESYLLYEGNHRI
                     WEEMYLNSLYLFSDRGIREVVYTAFSETDIDNLFDKGTKLGEQLNAFYRTDIFSLGNA
                     DNVVKGMTVEHYNSLEEKFKAGYDRYVTREQEKSTIGAWFNSTFSLDNTDLENLTTIE
                     EILANVEVTNAILNNSNAIVALTMCKTSMDAVVASSNAMDLLGQYILKVTAEPAVIKA
                     ILENNVIREAIINSTEAMTHISTNHESVRMIFENLEATQLLLANQDTLDLVLANTNVI
                     RLVFTKILKTHNSIFKLDMNPLITIITNMVNSTNIEYQDKIFPIVENMNTNFISIKDL
                     LSNNNLDKNNIIAIFQCIENNLSVITALSSDEVAFNSIKEIDLAIAKTAAKLAGIDNI
                     NINTSDALARSDKYMNAVASSQIAVNTIINNSGFLNIVISSSTAMSAIASSSTAMLSI
                     SKSPLALNKILKTEIGRTSLANSSYLQTNYSNISTSTQNTTYFAKGSNAVIDSGSRNS
                     NDTGGMVSSTTPNQCIFICLTIGSWSNGTNTTGTISHLQTGSQVATRTTTTGPIHQGT
                     GATAQYICVGGCKFTESSDSGAWGCMAYAK"
     gene            complement(17860..20079)
                     /locus_tag="MJF80_000378"
     CDS             complement(17860..20079)
                     /locus_tag="MJF80_000378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279059.1"
                     /translation="MRDRIYIVDINTKMYQIARYKQNDNNVSYNMRIVQDSIDVDLTG
                     YTALAFFNLPSGRVTQKNCTIEDSTVYTELSHIELSEEGDVISEITLYKNDKVVTTFS
                     TIIKVEKSINRNAIEDEPSWDIIKDILNVLSYEEERQENENVRKSNEEIRISSENVRI
                     GNENVRIESENQRKDSEVERCESEEVRKTQEVTREANEETRKTSETTREANEEIRKTS
                     EITREANEEIRKTNEVDRESREETRNTNEETREENETVRESNEEIRKTSEETRESNEN
                     TRKASEEIRDGSEETRVVNEVARQESETTREENEDLRKSNEETREENEEIRKSNENTR
                     KASEEVRNTIEETRNTNEEARKASEEIRKTSETTRNTNEEARKVSETTRETNEETRKT
                     NEIIRQNSETKREENEELRESNEGTRELNESTRQTSIINMQKKVDDKVDEVDNKIVEV
                     NTAKTDMTTTVSNKITEFEKRFNELENLDTAGEILQSKETTDGQIKDTLKERLTYDFN
                     KLDLKIEQLIAGGINVAFNKSYDTSEWIEVDGGFELSVEHNLVTEKILVSLIDKLNNK
                     SLITSYKIIDDNNIVLFNEVAIDIEVTVVNGGSAIELIKFTINDDIESLESAYSSIET
                     KRLLKDGLDKKANLEHTHDIYVEKVEGKELSTEDYTTEEKEKLSQLDTYKNKETKFNT
                     NGSIIEILDNNIKYITKFNTNGSISKEKYINDILINTTITNFKNGMIEETVTNEGVI"
     gene            complement(20086..22854)
                     /locus_tag="MJF80_000379"
     CDS             complement(20086..22854)
                     /locus_tag="MJF80_000379"
                     /inference="COORDINATES: protein motif:HMM:TIGR01665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBZ0279060.1"
                     /translation="MLRLYKANETNFKNNGLGILKDAFETKVTEELNGLFELEFTYYV
                     GSFLFDEIDYNKIVMADASPRLKNQLFRIYYISKELDGKILVKAEHISYDLLNNFIEN
                     LELKNVTCEEALNQIFRSCTEENRFVGYSDITGNKDFSISCVSPHNAISDIQELFNNK
                     SKLKRDNFNISLLNNIGESNNVLLAYRKNIIGLTATYDTQEVITKIYPYATKKSGKNK
                     KITLPEKYIESKYINNYPTQRIVAVDFSDDDVKNEESLRNKCKDYFIENNVDLPKVTY
                     KVEFVDLSTTEDYKSYKMLETVNMDDEIIVRDYNLGINATARVVKTEYNPVLKKYNSV
                     EIGDLVNHFKDERIDDLEEKIDKVQNNVDNIVIESDNFPDTLPEPSNVTALGLWSMIQ
                     LDWTFDNKLYYNYEVYASQIKGFEPDTIGYTNRIYVGQASSLLHEVKPMQTWYYRVRA
                     GNTHDNYNEFSNEVSATTRKLSDAAEYFEEAAIGHAVIRDLDADKINVGKVKGQYIEA
                     KNLVVVDGNSQTTLNIDSFGNVHIGATTFTLKGKSLESIIGGEIDDITQLEIFNKLTN
                     NGLAKGLYMVGNELYLNASYIKTGTLEGQFINGRNLTVRDNDGYTTLQVDSNGKVNIR
                     ANELSIGDKNNYESVLTSDQKAVFDALTGNRNCGIYLSGSRLYINADYIDTGTILCDR
                     IGASSSNPFILLFEGNGAKCALDATAQFGVGIGKAMRMKYNDYSYIYVSDDVISGYLD
                     GEEVFEFGYQDEKSYINTGLNTQHINPQIDGWYNCGSNRKAWDYLVCNNLNQLARTAA
                     TSTRMMKSTFNEEISNNCIDFVKSSLVQSDVFTPYNLKSMKNNNIEHRLQVDIDSSLD
                     NPISKYIFKDVSDEAGEGVYAQDITSHLAVLQLSLQKTISNFENYKDITSKKIEELTK
                     RIEVLESR"
     gene            complement(22856..23611)
                     /locus_tag="MJF80_000380"
     CDS             complement(22856..23611)
                     /locus_tag="MJF80_000380"
                     /inference="COORDINATES: protein motif:HMM:NF017520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HBZ0279061.1"
                     /translation="MALQYCVSENELVSFQYGEINSRDFGIVITDINELASPERRFER
                     IEIPGRNGSLILDEGCYSNFNLEIECYIDVDGKDINILASEIKMWLQTDFSYKNLIIS
                     TDPNYYREAVCINKLDLEEIIKDLGYFKITFECKPLKKELVNNLITINESNSKLINTG
                     MASYPLIKVFGNGDITLEINNEVIELTEVQGHIYIDCELMNAYKIDIHTNGVVNENSR
                     MFGDFPTFKHGENIITYSGDVEKIEIEPRWVVL"
     gene            complement(23772..23921)
                     /locus_tag="MJF80_000381"
     CDS             complement(23772..23921)
                     /locus_tag="MJF80_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279062.1"
                     /translation="MNNFLLNVIAGVIASLIFCLISKIFAKVKSHSVRKSGWEFDLKI
                     KFHKK"
     gene            complement(24049..29598)
                     /locus_tag="MJF80_000382"
     CDS             complement(24049..29598)
                     /locus_tag="MJF80_000382"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBZ0279063.1"
                     /translation="MSLELGTAVGYLDLDGGKFFKALADAAKQLSQFNSSTKTTGSAM
                     TAMGKQISDTANSLTQIGRQSALAASELNKIASTSSGPFSSLKNSIKETENELRTARS
                     TIDTYGQGISRLTSEIDKSKQKYTEIGNKIERYEQQLERCNSMYGESSEQSQRYREAI
                     ERLKNSQTQLGNEIENGEDSLTDMRTAMNNAEAEANRLSDSLRSMPFDAIGGKMRDIG
                     QTLTSTVTTGLIAMGTAAVTAATNTEQAMSVVNSILQLNTEKVKGGKSEWDAYSSTLK
                     AGANEIGMAYDEYANAAYNAISASVKQADVTEFLAQADKLATAGLTDLAKATDVLTTV
                     QNAYGMSQKDMAHVSDVLIQTQNKGKITVDELASSMGKIIPTAKSLNVSVEQLGAGYA
                     ILTAKGIAGAEATTYMNAMFNELGKSGSKADKILKKLTDKGFADLQKEGKSTADVLLI
                     LNDYAKKNSLSLSDLFGSAEGAKAANVLLGDAVNETTGEIIEGTKSADSFNQMLGNMK
                     NSAGLCDKAFEQLDNTTKTKLEDALNSAKNMLSDLGEAMKPIISKVAEAITLFMNWAS
                     SMIQANPEIARIIVSIGAVVAAIGPLLVVLSALIINIPKIIALFGLAKGAIVSFGAAI
                     LASPAGIAVLVGVIAGLITMIGDNANALEFLQDKFGVFGTVLGTVCEFVAGITQMTFG
                     NLLILLKGVGEIIVALLSGKVWEIDDIWGKTWANIENNTAKAMSNIGAETSSALEKIK
                     NSTASQLSSVTSTFDLIMKEWPKLTRDNVGQVASTFAENLQGLDNQSIAILRGTSDSM
                     AVLFEGIYQGMDSTAATEKFASNLESMVISGKYSTETLQQDIDNAMRLINENVKDSSN
                     VLKQNATDVFNQFKTIGQAGFEGMAENVAGVMQGMDEQTISALTSMGGTWSDILKGVA
                     TDGSMSAEQMKNAIISNFNSMGIDGATLIDNLRKESSTYWKAMEEEANKAGSEVAKSF
                     DQVPKEVVTTLKNNGTESEEEILKLYNLYQQLPPEVQTYIKTNNMEALTGAQNVNDIL
                     AKIPPEIITNILSNVQGKEDVESTQMLLNALPKEERIKIETNVSGKENVTSVKQEIEA
                     LPKNANVNTNVNTGDSKEKLEEVSNKTKELNNVTGTLKVDANVSSAASKVSGLKTSVT
                     KDLNTTQANANKSTATMASNATNNFSKMASGAKSGTSKVATTTDADFKKANQSIQQSS
                     TAMYNGAKQSFTKLAQVAKQAGSDAYNGVNTSFSRLAQVVAISMSNAVTAANNSKSKF
                     KSAGSGAGQAWIDGFNSKKSAMRSAVSSVPKSVSFSFRSDEFIESNIKMKDSLAAGIN
                     KMSTFNNTSMRGSIFDEIMSTMNGTAKVFAGSVSQFKKAGENSAKAYLDGLSNIEEGL
                     TNTLRTVQAIIGNGNIKEAQEIKKFNKEVKKLQEEKAEEIAELDKEHKKKQKERNEKE
                     NEEIKEAEAKKYKNKKEKLAALKKIKDKYNKKSVKDQEEYREKLEKINKKYDKKEKED
                     TEKHQEELQKLREEKIKAERDFNKKYNDIKEDYAEKVANLDKKYIEDQKKLNEEYQKI
                     YDSRVKSLMDYTDLFSSVTFEEIDNDELMENLEEQVDVLRKWDTDMADLSTKVGKDLY
                     EELLAKGPQAHNEIEAINKMSKEQLEEYERLYQEKKKIAERRAKEDTEDEKYRIEKEI
                     EELKKTYDAEYRKLGQEMQRSIEEQIESFKDKFGVVPAMFMETGKDSMQGMIDGIKSM
                     QGALGKVLSDIVNSINKQLSNIGFKDVNIPTGGRKEAARFRNEIENLQSIAYSDTLAR
                     GIASNNLLKDATVNINNNSKVDSEKSSDKKVELTLHIEKFINNTKQDIEQLGSEIAFI
                     TNRKLKF"
     gene            complement(29684..29998)
                     /locus_tag="MJF80_000383"
     CDS             complement(29684..29998)
                     /locus_tag="MJF80_000383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279064.1"
                     /translation="MALFGDKVDKEDKKKLKEQEEIQKFMNKYQLQDLNEKDLTILRR
                     IADDLFATGLIKTGLALSFTKIEEQTKINYLSALVEQNWMIIRQLSQLNSNIEKLVED
                     KK"
     repeat_region   30175..30529
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=30307..30334
                     /rpt_unit_seq="tttacataccacttagttaatataatac"
     gene            complement(30787..31329)
                     /locus_tag="MJF80_000384"
     CDS             complement(30787..31329)
                     /locus_tag="MJF80_000384"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279065.1"
                     /translation="MKINKKILSLVLSLVIGIYAVGCSSPSSSDDSSKKDAKTEQKKD
                     EKKEYKFGDTIEMKDYDEGTYKLTFEGVRSTDERNEFAEIEAKKVVFVDFNYENVDCK
                     EAVFVTEGIEYQVADGDGNVLQTYPVDDSTRMQQETTAGTKSKGTTAFAITSDSNAVK
                     IIVKDKVNNKVMGEINATLE"
     gene            complement(31417..31626)
                     /locus_tag="MJF80_000385"
     CDS             complement(31417..31626)
                     /locus_tag="MJF80_000385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279066.1"
                     /translation="MYNKMPSEILRIKDEYTSFCFDEACMFLVDAIKNDKKLKFKENS
                     NTSKENIKRTTFVDMALKKKEKDRK"
     gene            complement(31607..32083)
                     /locus_tag="MJF80_000386"
     CDS             complement(31607..32083)
                     /locus_tag="MJF80_000386"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279067.1"
                     /translation="MNELKVTSLEELKKIKLTEVIEVGRFLDGTMLVAEVKQPDLMAL
                     AMAGKIPNSLMTASMSLVEEKESKDSTGEKVLKKMNDESNFSKEMFEMMDIVAKEVLV
                     NPTYAQIKKIGLELSIEQRLTLFNRIQGGTKSLENFHQESTDIEDSKSSDNVQQDA"
     gene            complement(32143..32949)
                     /locus_tag="MJF80_000387"
     CDS             complement(32143..32949)
                     /locus_tag="MJF80_000387"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279068.1"
                     /translation="MAVQILNQYPLTDVVLVQIETVEQNPVTYTFDTSDEIGTEEIIS
                     EGEELTLKIKKKIIANRAAEDTSLGYDLTLKDNVFCPEILQIMQGGTIEKEEDGSFKR
                     YLAPEVGKTFSKKSFKTIIYSSVVGPGGDTGQFAKTTFPNCKGKSVPLNFKDGEYYSN
                     EYVINSRPNTGQSPYEVEIVEELPNGYEATKVFLDSASVSGATAGDKKITGLTAGKIY
                     KVTVNSNIKYTLADGTLTDTESDKAALTGTEIIGLTNGETYKVEEVSVSI"
     gene            complement(32955..33299)
                     /locus_tag="MJF80_000388"
     CDS             complement(32955..33299)
                     /locus_tag="MJF80_000388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279069.1"
                     /translation="MFKKIYKHLKNKGFNVYSIGQHQGLCIEPFLVIFEKGPLQTTEK
                     NIIKDLFEIYVFYPIGQYSKVSEYKISVESVMDEIVGIKQAYEALPILIDDEKQAYFT
                     RLSYYENKQIRR"
     gene            complement(33307..33741)
                     /locus_tag="MJF80_000389"
     CDS             complement(33307..33741)
                     /locus_tag="MJF80_000389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279070.1"
                     /translation="MSNGFKFDTSKLLNALVSREMKTKAALGAYADTSSQLLESTAKN
                     DRPWKDHTHDAKNRLHGSWEWQGDTIRIALSHGVDYGLYLEKGTGPHVIEARPGSYLF
                     WDGASHPVKKVNHPGSRPYPIIMPTIEKCAPSIIRGLDVILK"
     gene            complement(33743..34105)
                     /locus_tag="MJF80_000390"
     CDS             complement(33743..34105)
                     /locus_tag="MJF80_000390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279071.1"
                     /translation="MIAQKVKPKIIKAINKMPTEATVKRVGVNEFGEPSDEENIVCNM
                     IGLYHEGSSSISQITKDKGVVIKDKEQYLMVVCDEDTVKIKQGDFLYLDDNKFIIQDL
                     GNQNRMNIYFDLKLGKVR"
     gene            complement(34102..34356)
                     /locus_tag="MJF80_000391"
     CDS             complement(34102..34356)
                     /locus_tag="MJF80_000391"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279072.1"
                     /translation="MDLEILKNIKTELREEQSPFFSDDEITYYYNKNNQDFNKTMYEL
                     CILKAENDSITLPGGLSMPENKLYWLTLAKKFKTNGSRCL"
     gene            complement(34382..35350)
                     /locus_tag="MJF80_000392"
     CDS             complement(34382..35350)
                     /locus_tag="MJF80_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HBZ0279073.1"
                     /translation="MAVTKLSDVIVPELFNPYVINKSMELSSLYQSGIITNDASLNAL
                     ASQAAPVVNMPFFEDLSGESEQIIEDADLTPNKITSSQDAAAILRRAKMWAATDLSAA
                     MAGKDPMAAIASLVGSFWARDMQKELIAVLNGVFLAANMTTNKLDISAGTGNAAKWSP
                     SAFIDAQQLLGDAQGQLKAIAMHSATKSALKKQNLIETIRPDVGPDFEVYQDKLVIVD
                     DGCPVSKEGVYTSYLFGSGAIALGNGNPVGFVATEIDRDKKKGSGVDYLINRKTYILH
                     PRGIKFTNASVAKTEGPSRLELAKGENWTRVYEPKQIRIVEFKHKL"
     gene            complement(35368..36003)
                     /locus_tag="MJF80_000393"
     CDS             complement(35368..36003)
                     /locus_tag="MJF80_000393"
                     /inference="COORDINATES: protein motif:HMM:NF018515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBZ0279074.1"
                     /translation="MEELLKKLGYQDTDIKNIIEGMKKEKIYTSKEENIDERYNKLKE
                     QKLALEEQIKGANDTISDLKKNSKNSEDIEAKVKEWENKYNELDSTSRAKIKNMTIDY
                     AINSKLSGVNEKYRKLLCKEFDTSKMEVKDTGEIIGLDEQFKDISETYKEWFESSTPS
                     NTGSPGNFPRKSNVVNNPFIKETFNLTEQGRLLKENPDKAKEFAAQAGINL"
     gene            complement(36139..36315)
                     /locus_tag="MJF80_000394"
     CDS             complement(36139..36315)
                     /locus_tag="MJF80_000394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279075.1"
                     /translation="MCNKFEDDKYLEPFSHPRWKDIPWGETIREVERTEEEQAQIRTK
                     FRKKLKEIGVIKED"
     gene            complement(36345..36560)
                     /locus_tag="MJF80_000395"
     CDS             complement(36345..36560)
                     /locus_tag="MJF80_000395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279076.1"
                     /translation="MLTIEEFIKLPDEEKGDAYKELSPHDMFLWRTQYSPIGFEVIGH
                     EEISEEDRIKNKKKFREHLKKIGVMEE"
     gene            complement(36561..36800)
                     /locus_tag="MJF80_000396"
     CDS             complement(36561..36800)
                     /locus_tag="MJF80_000396"
                     /inference="COORDINATES: protein motif:HMM:NF015461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279077.1"
                     /translation="MYKLGEIVTNYDYTSTTKGSTYNSEGQVQMYILNSQKGKDVSKY
                     NPKEQEVLYKRGSMFKVIEIERIKGTIHILLEEVY"
     gene            complement(36832..37008)
                     /locus_tag="MJF80_000397"
     CDS             complement(36832..37008)
                     /locus_tag="MJF80_000397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279078.1"
                     /translation="MDNKFEDDKYSEPFSHPRWKDVPWGETIGYRDIPEEERKKYKKI
                     LREHLKDIGVLKED"
     gene            complement(37001..38419)
                     /locus_tag="MJF80_000398"
     CDS             complement(37001..38419)
                     /locus_tag="MJF80_000398"
                     /inference="COORDINATES: protein motif:HMM:NF015461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279079.1"
                     /translation="MANKFNKAMKNAERARDISSNRTTKKIRKLYKDIANEYAKKLNR
                     VNSNTLTEQYLRESIIYLNKEYDRLGKKLKKDIESEISKVIKTTTDEQLSFFNNICDN
                     YSVNLKPQFTDMFSKVHEDVLRQVISGSMYKDKLKLSDRIWSNIDKTKKDLDYIVSRG
                     LAEKRGSYDIAKDLEKYVNPKVKKDYDWSKVYHKSNKKIDFNAYRLASTYITHAYQKT
                     AKESCKKNPFVKGIKWMSSHHPRMCKVCADRNGKTYIPEELPLEHPLGKCTFEYDIPM
                     SMEDIGKELRSWIDGEENSKLDEWFDEYGLDFAGIEGKANKNKEPTEDEMLALYKYMG
                     GDAYKINEKLRRNIKLTEEDEWFINNLDRVLDKMPNYEGDVTRSLYFYNKESLEAFLI
                     EHEIGKTIQYSEFISTTKGKTYNPEGQVEIYIFNSKNGKNISMYNEKEEEVLYRRNSK
                     FEIIELEEMNGKYYILMEERNG"
     gene            complement(38419..39903)
                     /locus_tag="MJF80_000399"
     CDS             complement(38419..39903)
                     /locus_tag="MJF80_000399"
                     /inference="COORDINATES: protein motif:HMM:NF016988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBZ0279080.1"
                     /translation="MLDLIDIIQMELTGLYGQEVIREMGEIIRLYDKYEGTGQHWIEE
                     EQDYKQTRKKTNYIKKLIKEEARFLFGKTPIFTIKPENDLDKDKAEEINQCINKILKK
                     NLFSDKLIKAARDCFIGKRIAIKLHADKESKSLKIMFVPSLEFIYEPFDNQFDELKKI
                     VFFHQTNQEVEKDKQRIWKQKYEMVNDKCILSEGIYNGYGLCTEEIVSDTDLKLSGIP
                     CYVILNDGLLGDLKGESDIEEIFDNQMAYNKLASEDIDTLKKGMNRIIYGVDVEEESS
                     KHFKIKPGAYWDVETSQTADQKQAQINTIPTDFGYDTRIENSLNRIKSDMHEVLNIPL
                     INNQDLKGMMTSGKSMKALYWQLITRCEEKMKSWGPALEWLIQAMIEMIEVYNIVKIP
                     ILDKDSYEVIVENQYPLQEDEDSEKLLDIQQVNAQAMSRKTFIKKWNNSNDDIADEEL
                     QQISIERQILEESFNLEEETEAIDTEESDEVTNEKESKEVVDDE"
     gene            complement(39914..41179)
                     /locus_tag="MJF80_000400"
     CDS             complement(39914..41179)
                     /locus_tag="MJF80_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBZ0279081.1"
                     /translation="MMDRKISEIINKNFYEFWKVSNSNKYLYHVLKGGRASAKSTHIA
                     FWLTMAMVKYPVNTVCFRKVGNTIMDSVYEQLKETIEIFGLTHLFQFKKSPMEIIFIP
                     RGNKFIFRGLDDPQKIKSIKSAKYPIAFAWFEEVAEIKTEDELSMVINSVLRGELPDK
                     LNYKIFLSYNPPKRKQSWVNKKFEIHVLPKNTYVHHSIYLDNPHISKAFIEEANEIKI
                     RNEFKYRWEYLGEPIGSGVVPFSNLEFKTITNEEIFQFDNIRQGNDFGYATDPMAFVR
                     LHYDKKKRVIYFIDEIFGVKMSIRELASKIKSKGYDDFSVICDSAEPRSIAELREYGI
                     KALKAKKGPGSIEFGENWLDDLQAIVIDPNRTPNIAREFENIDYQTDKDGNVRAKLED
                     KDNHSIDATRYALELDMKTHGRERKYNSR"
     gene            complement(41169..41609)
                     /locus_tag="MJF80_000401"
     CDS             complement(41169..41609)
                     /locus_tag="MJF80_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBZ0279082.1"
                     /translation="MADLTEKQKRFCDYYIETGNATEAYKKAYKNNNQRTSESNGSRL
                     LSNDKVKNYIDERLKQIESKRIADAKEVMEYLTKILRNQEQEEVVIVSENGPEIIKKD
                     VSIKDRNKAAELLGKRYALWTEKVDLDGNVGVTIIDDVGNLNDG"
     gene            complement(42035..42211)
                     /locus_tag="MJF80_000402"
     CDS             complement(42035..42211)
                     /locus_tag="MJF80_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBZ0279083.1"
                     /translation="MEKRILKVLLSKSGSGSLSPKISLPATWIKEMNITQEEREVEVY
                     FENNEIRIKKKDLD"
     gene            complement(42621..43121)
                     /locus_tag="MJF80_000403"
     CDS             complement(42621..43121)
                     /locus_tag="MJF80_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279084.1"
                     /translation="MASKVKKEFFMATKKHLENYKQLHINIESLKLQIKNLKEFHLGD
                     FMQGLSYDSIPISKTNSISNQVENELINLEEKIIEKQIELYEMEALKYTIDVSISNLK
                     PIHKQIIRYRYIEGLEWSLIVDKVYLEERQLRERANQAISSISIALFGKKALIEQEPL
                     FKMLDL"
     gene            complement(43140..43556)
                     /locus_tag="MJF80_000404"
     CDS             complement(43140..43556)
                     /locus_tag="MJF80_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBZ0279085.1"
                     /translation="MSKYNNKKIVIDGIKFDSKDESEYYLYLKEKKENGEIKDFGLQQ
                     KFELQPKFKKDGKSYRAITYTVDFAIYKWNGEVVYIDVKGYSTQQGELRKKLFDYKYQ
                     DKKLIWIAKSKKYGVDGWIEYSELKKKRKENKKKVA"
     gene            complement(43583..43780)
                     /locus_tag="MJF80_000405"
     CDS             complement(43583..43780)
                     /locus_tag="MJF80_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279086.1"
                     /translation="MNKFQKAVSQMVKQEEKENLLQGYENCIVGRSISSSIRRYVKGF
                     EKFGYSVHEVYEFINDINKYE"
     gene            complement(44193..44555)
                     /locus_tag="MJF80_000406"
     CDS             complement(44193..44555)
                     /locus_tag="MJF80_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279087.1"
                     /translation="MFKVERYFSGSVVDNLIEDDLTCRNYLALYCCLLGITKNGKKIY
                     PKPEKMLAEFGVKKDRKIKKELPVRIRNVNTGEVKEFESIDGAACFLRLKYQAVYQAI
                     KKKTKTRSGWKVEYIKEE"
     gene            complement(44585..45004)
                     /locus_tag="MJF80_000407"
     CDS             complement(44585..45004)
                     /locus_tag="MJF80_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBZ0279088.1"
                     /translation="MNQVVLVGRLTRDPELKYIPGTGTAVASFTIAVDRNYINKEGKR
                     DTDFIPIEVIGKSAEYCANYITKGKLVALEGNIRVDNYQTQSGEKRPFTKVSTKSVQS
                     LESKSKSSNSYKESVQDGTIGLDPQGFEIIDDDELPF"
     gene            complement(45024..45914)
                     /locus_tag="MJF80_000408"
     CDS             complement(45024..45914)
                     /locus_tag="MJF80_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBZ0279089.1"
                     /translation="MDKVIIEKGNILSDGYGLMPRLVARDRWLTVGARMLYSYLSSFA
                     GINGTCFPTRDLICYELNISKDTFTKYKNELELSGYIKIHKNKSKQGKMQNNIYEIVF
                     DRTYIDECISRRGVKVNKKKKKPCPKNIDMEPCPTFPDTTQPDTENVDTNINSININS
                     FNSMYIDKPVDNYLKEFKKLYEENIGVVYPVTAEWLLEVSKEVDIRVFKRAIEICAER
                     MNMNLSYLKGILKKWKDANITTYEQLESYKLQHENKKSKKPNSVVSKNKFANFEQTFT
                     QYSNKELDEIIKKSQKAKFK"
     gene            complement(45933..46571)
                     /locus_tag="MJF80_000409"
     CDS             complement(45933..46571)
                     /locus_tag="MJF80_000409"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HBZ0279090.1"
                     /translation="MEVNNIYIKLMDVRIKFNKLDIKKSGQNKFANFKYFELADFLPQ
                     ATELLQEAKLCPIVTFTNDYATLTLINGEKPTEEIIFTSPMRELQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITENDTFDATSGKEDYKRNDLTNSSIKASENGQIKISQNQIKRL
                     FSIGNTIGKDSDRVKSEVYYKFNKEVKDLSKQEYDQICVGYEKLQREKGIIK"
     gene            complement(46558..46839)
                     /locus_tag="MJF80_000410"
     CDS             complement(46558..46839)
                     /locus_tag="MJF80_000410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279091.1"
                     /translation="MKTKNEIIKDLEDRLFLLRFTTVDEVDWDVKFGQISALEFCIDK
                     HRKGCTLQQFKENLEEYKLQGNYGDYIDGFVSVLERNIREMEGEIDGSE"
     gene            complement(46918..47160)
                     /locus_tag="MJF80_000411"
     CDS             complement(46918..47160)
                     /locus_tag="MJF80_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279092.1"
                     /translation="MEAARLIAIGQIKQAEKEICKLQGTKNNSSLMWWEAVKFASQNI
                     LEGLEHDIELEASIEFREAMMYQEELEKDRPIDVQI"
     gene            complement(47327..47470)
                     /locus_tag="MJF80_000412"
     CDS             complement(47327..47470)
                     /locus_tag="MJF80_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279093.1"
                     /translation="MLSLNTNKNNIVTLKKDGKAIADIVFKDIKTGKKISVGVLNKKV
                     LVK"
     gene            complement(47457..47849)
                     /locus_tag="MJF80_000413"
     CDS             complement(47457..47849)
                     /locus_tag="MJF80_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0279094.1"
                     /translation="MDISESIAKQFSDSLKSLIEIEINKQETDRVKSQTVEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTVFMRRLVSTGLIKSLARGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIVKP"
     gene            48019..48255
                     /locus_tag="MJF80_000414"
     CDS             48019..48255
                     /locus_tag="MJF80_000414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279095.1"
                     /translation="MNKDELNKICDEVYNRILKSEYSDETLLRNKISEFISLKEKSAS
                     EISNTELCMFFYFENIAFTKNYVKEVLSEILLDK"
     gene            complement(48213..48395)
                     /locus_tag="MJF80_000415"
     CDS             complement(48213..48395)
                     /locus_tag="MJF80_000415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279096.1"
                     /translation="MNEKDTKITEKDLHYITDYLLKESDIGKRLALLEEKTKPIKIDE
                     ISDYLSKRISDKTSLT"
     gene            complement(48443..49258)
                     /locus_tag="MJF80_000416"
     CDS             complement(48443..49258)
                     /locus_tag="MJF80_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBZ0279097.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKRKKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFDEMEKALKNEQTKLPTTYKEALQHLIEQVEANEKLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQYQGKGYTHS
                     ETIDITRSDGMTDVKMTTKWTQKGRLFLYDLLKVNNILPDIEKEYSYQTSMLG"
     gene            complement(49289..49504)
                     /locus_tag="MJF80_000417"
     CDS             complement(49289..49504)
                     /locus_tag="MJF80_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0279098.1"
                     /translation="MYVNRLKGLMKENQHTQKFVADLLGLSLFGFRLKLNGKNEFKAN
                     EIKKLSELYGVSTDYFFSELVAKMAIK"
     gene            49685..50230
                     /locus_tag="MJF80_000418"
     CDS             49685..50230
                     /locus_tag="MJF80_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBZ0279099.1"
                     /translation="MFRLKELREEKGISLDRLSMDLNVNKSTLSRIENGLREPKQSFI
                     EDCSNYFDVSTDYLLGKTDIKNPNQVVKSLNPIFSKRLRELIKEKGVTLNILGKKLEI
                     STDLLSKYENNIISPKSDDIIKLAHYFDVTTDYLLGTTLVRNHIDTVAAHKANPHEDL
                     PEEAQEQLNDYIEFLMNKYKK"
     gene            50239..50655
                     /locus_tag="MJF80_000419"
     CDS             50239..50655
                     /locus_tag="MJF80_000419"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HBZ0279100.1"
                     /translation="MSEEQYLENRVNKQINWYSSKSSNIKKKFFTIQVIEIIIAGIIT
                     ALSKFSFENEHIADIITILSIVVIIFSSLRNLFKWQDLWIKYRYTAELLKREKFLFLT
                     RTNIYINENAFNLFTERIEDILNRENENWTELNLNS"
     gene            complement(50668..50889)
                     /locus_tag="MJF80_000420"
     CDS             complement(50668..50889)
                     /locus_tag="MJF80_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279101.1"
                     /translation="MQVKKFRKKPVVIEAYQTDIEFIIQTLEGPLKASIGDWIITGVR
                     GEQYPCKPDIFEKTYEAVDDEVPLTSFIV"
     gene            50953..51375
                     /locus_tag="MJF80_000421"
     CDS             50953..51375
                     /locus_tag="MJF80_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HBZ0279102.1"
                     /translation="MPKLYDYRVFISHAWKYGNDYDRLINLLNNANNFSYYNYSAPKE
                     KPLFPDGTPFSNSDIAKKITDKISPSQITIVISGMYVQYKDWIKYEIDESIRIGKPIL
                     GIRPYGNSNIPTYVSDNANKIVNWNTDSIVSAIRELVK"
     gene            51479..51985
                     /locus_tag="MJF80_000422"
     CDS             51479..51985
                     /locus_tag="MJF80_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBZ0279103.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            52225..53355
                     /locus_tag="MJF80_000423"
     CDS             52225..53355
                     /locus_tag="MJF80_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBZ0279104.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
BASE COUNT        16358 a         9407 c         5704 g        21998 t
ORIGIN      
        1 taaaataata atattttatt ttaatttttc gaccatgtca tctatatact ttttcaaacc
       61 ttgaatcttt ttatctgctt caatatcact agctcctttt acagctatgt aaaatttaag
      121 ttttggttct gttccagatg gtcttatagc tacccaagat tcatcttcca aaaagtattt
      181 taaaacatca gatttaggaa gattatctat accattttta taatcttttt tatcaactac
      241 tttaatacta tttatataat caatttgatt ttctctaaaa taagatatta tttcttttat
      301 cttttctaca ccttctatac cttttaaagt caaagatata gtctgttctt tgaaaaatcc
      361 atacttttta taaagttcta ttaaaccatc atataaactc ataccttttg aatagtaaaa
      421 tgctgccatt tcagatatta acaatgaaga aactacacca tctttatctc tagcatgagt
      481 cccaactaag taaccataac tttcttcata tccaaataca taacttctat ttttattttg
      541 ttcaaataat tttatttttt caccaataaa tttaaaacct gtaagaacat ttaatacatc
      601 aacattattt gctttagcta tatctgctcc aaattctgat gtaactatgg tttttattat
      661 tgtaggattt tcttttaatt tattattttc ttttaatcct tctattatat aatgtgtcag
      721 cataccacca atttgatttc ctgtaagtaa agcatactca cctgtagtag tttttactgc
      781 tacacctact ctatcacaat ctgggtcagt tgctattaca aggtctgctc ctttttcttt
      841 tgcgagtaat atacctctat ttaaagcttt tttctcttct ggatttggat aatcaagccc
      901 tgcaaaattt gggtctggat tttcttcttc tggaactaca attatatttt caaaaccaac
      961 ttctttcaat gctcttctta ttggaacatt ccctgttcca caaagaggtg tataaactat
     1021 cttaaagctt ttacctacat ttttaactaa atcttttctt ataatttggc tttttactgc
     1081 ttctataaat ttagtatctt gtgattcgtc caacaaaact accaaatttt tattagcttc
     1141 cgttaaggtt ggtattgtac tataatcatg tatactattt actttctctg ttattgcatt
     1201 tgcaatctct ggcataactt gtgctccatc ttcccagtat accttatatc cattatattc
     1261 tggaggatta tggcttgctg ttatgactat accagcgatt gcatttaagc ttctaacagc
     1321 aaaagataat tctggagtag ttctcaaact atcaaatata tatgctttta taccacatgc
     1381 tgccaaagta tttgcagatt ctatacagaa ttgtctagac ttgtgtctat tatcatgagc
     1441 tattactact cctctacttg tttcttcttt tgttgtgttt tctaatatat agtttgccaa
     1501 tccaaaagta gctcttctaa ctgtgtactt atttattcta ttcgttcctg cttcaattat
     1561 tcctctaaga cccgcagtcc caaattctaa atccttatag aatctatcct ctatttcttt
     1621 ttcattattt tttatattct ccaactctag ctttgtatct tcatcaaaat atgaaccctt
     1681 tatccactct tcataagttt tagtataatc cataataaac ccctctctac tgtatgtatt
     1741 tattgcaact gatgcataaa caagcatttt aatgtaatct ataactcttc tatatactta
     1801 ttaattgaat taataaaaat gtctgccatt attctatgac cctctttggt tggatgtaac
     1861 ccatcaaaat ataatttttt atatccttct agttccatat ttttattata ttctgaataa
     1921 aaatctacga aattagtatt aaatgtcata caaaattttt ttatccaata actatatgat
     1981 tctaagtttt gagatactat attaaaatca gtaaaacaac tccaatcttc cctaacattt
     2041 ggaatatctg gttttaatgg tatacctatt ataggtataa tattttttga atatgactga
     2101 tgcaccatag ccattatatt agaattaata atttcatttc ccgcaccagc aataaaatca
     2161 ttggtaccac ccattataaa aactatatca ggagagtttt ttacaacatc ttcattaaac
     2221 ctagctaaca taccacttgt tgtatcacca tttataccct tatttaatat ttctaatcga
     2281 gtctctttat ttacaatgtt tgtccatgaa ttacttcgtg atactccaaa tccatacgtt
     2341 aaactgtctc ctaagcagac tattttcatc ttatcccccc ttagatatat ttttatgttt
     2401 taagaataat ctaacagttt agttaattca tattttatac ttttttgtcc ttcaagtaaa
     2461 gttattctat tcactaattt aatgtagata tatatctata cttttccttc cccaatttga
     2521 agcttttcct tcactgttca taaaaatgtc tattctatta cctttaattg cacctccaca
     2581 atcttctgct acaaaagtca tacccagttt aggtatatat acttttgttc catatggtat
     2641 tacacgtggg tcaactgcaa taacacccca tcttggagtt gtaccagtag atgttattgt
     2701 atctcctgca tatgcagtgg caaccacttt catatgtttg cctcctctta tatttgctcc
     2761 tcttgatgct attaatactt tttctttagt tccatatttt ataatttttg tagttggctc
     2821 cttagatatt acttcttttg ataagacttt tttgactaac tttccatcat ggtaagttaa
     2881 attatagact agtttttttt ctccttcttg accttcttga taaactttag attttccttt
     2941 taacaaatta ctatcattta catgctttgt atcaaatgga attttttcaa tctcttcttt
     3001 tttactttta gttaccttaa caacttttat tttcatatca tcttttagtt ctgtatctaa
     3061 acttggatta atcttatctt cagaattata ctttacaccc tgttcatcca aaagttcctc
     3121 aactgttttc ttaaaggaag atactttttg ctcttgacct ttaacggtta aacttatgtc
     3181 ttctttattt aatatagaat atatacttgc caaacccatt aaaattgata cacttaataa
     3241 agatgatatt attatcctct tttctctttt ctccataata atctccttcc atgttcaaat
     3301 tttaaattaa tattattcat tacagtttta accttatatt atatatgtat attatagctt
     3361 tccaaattta tgtaactttt ttgtaacttc taaaattatt aagattactt ttagacctag
     3421 aaaagtccat aaatatactg tttttagatt atcaaagctt aatataatag tttttagtta
     3481 tgtcttaagc aattatctct tagactatga gatataagca taaataaaag aaagaaatga
     3541 cactcaactt aaaacacatt gtatgcaaat actgactatg gtaaaatgaa ctcataaata
     3601 aattggaatt ttcgtgatta ggaggagtaa agtgaaattt gaacggttaa gaaccagtga
     3661 aaataaatta tattacaagg ctatggaatt atataaaatt agtttcccat ttcatgaaca
     3721 aagaaaatct tctttgcaag cagaaatttt gaaaaataaa gaatatcaat tcaatttaat
     3781 ttatgataaa aaccagtttg tcggcataat tttatgttgg gaaacagatg actttatcta
     3841 tgtagaacat ttctgcattt ttccagaaat gcgcaataat agatatgggc aaagagcgtt
     3901 agagctttta aataagaaag gaaaaactat tattttagaa attgaccttc caattgatga
     3961 gatttcaatt tatcgcaagg cgttctatga aagagtaaaa tataagacaa ataattatga
     4021 acacattcat ccaccatacc atgaaaaatt taatgggcat aacctgattg taatgtctta
     4081 cccagaaaag ttatctaaaa tggaatatga taaatttaat cactatctaa gaaataatgt
     4141 catgagtttc taaatataat tcctatttat tgaactgtat aaaacttcat aattaaataa
     4201 aagagatgtc tcaaaataga aataaggctc ttttccattg tttaaataat aaagatatat
     4261 tggatttcat acaaagaagg atgcatcatg aactaaaaaa ttcatgatac attctctttt
     4321 tcacgcttaa agttaagaaa acaagtgaga ttttatagac aaaattacat tttatataac
     4381 aaactttatt ctatttttat aaatttaggt ttctattttt gtgtaataat acagataatt
     4441 cagatttcta ttttgaaacc tctatttttc taaataactg tttaagaccg acaaaaaagg
     4501 tgattcaaat ttttattttg aaacactttc ttttaatttg attaatcttt aagtcctata
     4561 ttaaattttt acttattctt tatttttcaa cattttttcc atatctaaaa tcttttcaat
     4621 tacatcctta aaaattggtt cttctctaag ctcattataa ctatcatcat taagtacaat
     4681 taacaagttt gtatatatat taaaagtata taaattttta gactctacct tatcaaaaaa
     4741 tctcatttga cttacatcca aattttcatg tataggatat ttttctaata tttcttttag
     4801 actatataag gctttttttg cttcctcttt catgttaaaa aataaaaaaa ttagagataa
     4861 ttccatataa tccatataca tactaaaaaa tgcaccttta tcttcactga gcattttttt
     4921 tatttctaaa gaaagatttg cataccccag caacttattt tttatatact ccatttctct
     4981 ctcaattgca tttttattaa aatttctatt ctccaatttt ttctttaatt ccttgtattg
     5041 atttttgtat acattaatta ttcccttaca atccatgctt atattaaata cattactaaa
     5101 caatttattt tgaagcaata tttctgcttc ttcaagtcta ttttgattta aatatatcat
     5161 agctaaatta acacttgtat cccctattga cttatgtatc atatttaaat acaactctgc
     5221 cttttcataa tcctccaaca tacaatatgc gtttgataat ataataagag ctgtttcttt
     5281 tatttggata tcatctgtat ttctcactat atcttcaaga atattcgttg tttcttttac
     5341 tctgtcatta agttcactct taccaaattt atatgaatac atctgataca gcgaacatat
     5401 ccttagcttc aataaatagc ttttaggata cttctctata tacatattgg aaagcttttt
     5461 tacagattct aagtcttgtc taaattcttc ttttggttct tctgttgata aatcaatttc
     5521 aattctagca aagcctgatt ctaattcttt ataaattttc attacttctt cttcacttaa
     5581 atcaatcttg taatttaaaa gcttatcaat agacacatta aaaaagtctg ctaataaagg
     5641 caataattct atatctgggt aagaatttcc actctcccac ttagatactg ctggtgttga
     5701 cacacctata aactttgcta attgttcttg agttaaattt cgttcttttc taagacactg
     5761 tatgacttta cctattttaa tttgcatata tacctcccta gtacatctgt tgtaatttaa
     5821 gtatataaaa aaaatattta ctagtaaatc gagggaaagt taacaaaatg tctgtaattt
     5881 aaccattggt tatattttaa tctaaaaccc tatctcattg tcttgaaata taaaatctat
     5941 taattaagtt taagtataac ctaattaata gattcacatt aaatagcatt taaatattta
     6001 tattttctct ttactacaac ttactatatt taaactttca aggaatttta aataaaataa
     6061 attccatata caatgataat aatactgagt gttaataatt tatttataat aacacccacc
     6121 tattcaataa atggcattat agcaaaaaat cacttctggg ctctctcttt tctactttgc
     6181 atatacataa tccacttttc aaatattata tataaataaa tttaaagtaa atatatcatt
     6241 atattttttt attacaatat ttctatttta ttatttaacc aacaaattat atcctcaaaa
     6301 acttcttctc tatttatttc attcaacaat tcatgccttc catctttgta aagtttgcaa
     6361 gttacatctt ttactcctaa ttttatatat ctatccctta gtcttaagac tcctttacca
     6421 ttttttccta tggggtcttt atctccagat attatgtaaa taggtatatc aagtggcact
     6481 ttttttaagt tttctttatc ctcaatttct tttaaccctt gaactaaatc ataaaaaaag
     6541 ccacaactac acactactcc acaaaatggg tcatttatat atttttcaac ttgttcttta
     6601 tctctactta accagtcaaa cttagtcttt tcatttcttc ttattatttc gccgccaaat
     6661 atcaaattat ttattttatt acttttagaa cgtcttccat actttttaat ctcacgattg
     6721 attatcagat gagcaagatt tagaatgatt ccttgctttc catttgaacc acatagtata
     6781 agaccagata aattattact gtaatccatt atatatcttt gacttgcaaa tgaacccatg
     6841 ctgtgtccaa atagataaat tggcaaatct tcattttctt tctttataat atttgttaaa
     6901 gtatacatgt cctctacaag acatctaaat ccttcttttt cagctaaatg tccaacattt
     6961 tctattatct ttgcagtttt tccatgacct ctatggtcat tgatatatac tatataacca
     7021 tttttagtaa gtaccttggc aaaagtctca tacctttgtg cagtttcagc cattccatgt
     7081 gcaatttgga taactgcctt tggttttttt atattttcat cttcccattt atatgtatat
     7141 atatctaaac cttcctctcc tttgaaagta aagtttgtac acttcataat tatccctcca
     7201 gattgatttt attcagtata ttaatttata tattatatta caagtatttt caagtcaaaa
     7261 tctttctaaa aaatttaaga attattaatt cctcatagaa gttatcattg gtacatagaa
     7321 gtttttacat aaaattatac aataatttaa ttaattaata gttacataaa aaagtaatac
     7381 tttaatatat tttgacacca tattgacatt agaattaatt catgatattc tgttttcgac
     7441 aaaacaacat ctaaaaataa aggagatata tttcatggat tattcaaatg aactaaaaga
     7501 gctcttttta atgaatcaaa catatgcaac tctttttacc cttacaaaca aaatacaaat
     7561 agaaggagat aaatattttg gtatacttac ttcaagacag tatatgacta ttttatcaat
     7621 tcttcattta ccagaggaag aaacaacatt aaataatatt gcaagaaaaa tgggtacaag
     7681 taaacaaaat ataaatagac tagttgctaa tcttgaaaaa aatggatatg ttgatgtaat
     7741 tccaagtcct catgataaga gagcaattaa tgttaaagtt acagacttag gaaaaaaggt
     7801 tatggtgaca tgtagtagga ctggaataaa ctttatggca gatgtatttc acgaatttac
     7861 caaagatgaa ttagagacct tatggagttt actaaaaaaa atgtatcgtt ttaatggtga
     7921 agaacaagat ggctttgaag aagatgctaa ttttatggaa tatgaagaaa tagataaaat
     7981 taaatctgaa gcacttgaag aatttgcaaa aagaagaaat agagtaaata aaaaagatta
     8041 atactatcgc atctataata ccactttttt atagtacaat attttatata actataatgt
     8101 catttttatt ttttaacaaa acaatcatta catagattag gtgttcttag gaactctttt
     8161 tctgtttttt atacaaaaaa aggagatgat tagactttta ttttaaagca tctccttttt
     8221 cgctattatg attattctac tcctaaaatc atatctttta atttttgtaa atcattaatt
     8281 ggttgactac aactattact ttgacacacg taataactag tcttatcatc cttcaatata
     8341 tagttattta aaaaacctat aatagtgttt tcttcattgt atttctttgc taaaaaagtt
     8401 gcatttggta taaaattttc acttatcaat tctttaaatg caattaaatc agaatcttcc
     8461 ttaaaaatac atataatttc cttagtagaa taaagttcaa acatcaatga aagcatataa
     8521 aagctgtagc cagttggaga actctttaca ttatccacat acagttttaa ttgtttatat
     8581 gacatttctt caagtctatt atctccagta attttcgcaa gtcttataag attgtataat
     8641 tgaacagaat ttcctgatgg aatagctcca tcatacaaat cttttggtct tgctattaaa
     8701 ttttcactat ctttaccata tatataaaat ccactttttt cataatccca aaaaaggttg
     8761 atacagcttt cattcaaatt taaagccttt tctaaatatt tcatattaaa tgttgactca
     8821 taaagctcta tatacgccca gattaaaaaa gcataatcat ctaaatatgc taaatagtcg
     8881 gaacttccat ctctatatct agctaataat ctacctgact catttacaag attattattt
     8941 ataaagttaa gacacttatt tgaatactct aaatatatgt cattttttaa agtactatat
     9001 gcctttgtta aagcaactat catcaatgca ttccatgatg taagtatctt gtcatcttta
     9061 tgcaaagatg ttctttcttt tctatattca aatacctttt tactaaggtc agctatcttt
     9121 tcattatgtc tctcatattc tttatttttt ataagattag gaatactttt gccttcaaaa
     9181 tttccactac tagttatatc aaaataatta ttaaagaaag ttccatcttc ttcacccaaa
     9241 acttcaatta tctcaagagg attaaatgta taaaatttac cttcttcacc ttcactatca
     9301 gcatcctgtg ctgaataaaa cccaccttct ttatccttca tctctctaac tacataatct
     9361 atagttttta tagcaatttc tttatacaac tctttctttg ttattttata cgcatctaaa
     9421 aaagctatag taagcatagc attatcatat agcatttttt caaaatgagg agccaaccac
     9481 tttttatcag tagagtatct tgaaaaccca aatccaatat gatcaaacaa gcctcctcta
     9541 tacattccat ctaaagtttt ttccaccatt tttaacacat ctttgtcttt ttctatacta
     9601 taatatttca ttaaaaacat aagattctga ggacttggaa atttaggagc attaccaaaa
     9661 ccaccatact tttcatcata aatagcctta aatactctaa ctgatgaact caacatctct
     9721 tttgataaat caccctcagt attttttaca ccaaaatcat ccttaagagc ttctattatt
     9781 tcatctccag attttattaa tatatctctg cttgtattcc acttttcaga tacattctct
     9841 aataaatcta taactccagg tctattatac cttgaatatt ttgggaaata tgttcccgca
     9901 aaaaaaggtt ttttatctgg tgtcattatt atggtcattg gccatccacc actacctgtc
     9961 atagcttggc aaactgtcat atacacacta tctacatcag gtctttcctc tttatctact
    10021 tttattgcca caaagtttct attcattatt tcagcaactt cttcatcttc aaaagattct
    10081 ttttccataa catggcacca atgacatgtt gaatatccta cacttaaaaa tattggttta
    10141 tcctcttctt ttgctttttt aaaagcctca tcattccaac tataccagtt tatgggatta
    10201 taagcatgct gtaaaagata tggagatttt tcatttatta gattattagg ttttctatta
    10261 ctgtccaaaa taccacctca tttgttattt tattatatgt tttgcaataa acaaatagat
    10321 atacacttat aataagtgta tacccatttt taactataca tatttaattt ttcatataat
    10381 taattcttct agtaaataat ttttataact actaaaacaa aataaaaaat atcaaattat
    10441 ttttttaaag gtttttcaaa agaaggagga ttaatatcta tcttagtatc gtgatttggc
    10501 cctggagtag acaaatcgaa atataattta gaatcttctg taactccaaa atctctattt
    10561 gaacctgtat ctttaacttt atttaaagca tctctaaata gagcattatg tgcttcttct
    10621 ctattaagta agaaatctat tgtttctttt acatatttat catctatttg tctatacaag
    10681 tactcataaa ctaccttagc tctttgttca gaagctatat ttgaaagtaa gtcagctact
    10741 aaatcccctg taactgtaac gtaattagca gtccaaggag ctcctgatga gtttattaag
    10801 actggtgata gaccagttaa tacatgtgtt tctatttcgc ctgtattaac tgcattataa
    10861 tcaacatcat gtccatttaa taaatttata gtttgtgcta ccatttccat atggctaagc
    10921 tcttctgctg ctatatcaag aaataaatct tttatttgag ggtcttttat tctaaaactt
    10981 tgggacaagt attgcatagc tgcttttaac tctccatttg caccacccaa ctgttcttgc
    11041 attaaaacag catattgagg atttggtctt tctactttta cttcttttaa taatgcttta
    11101 tcgtgtttaa acatatgaat acctccgtaa attattattc aaaattatta tttacttaag
    11161 ttgtaatatt attctaggtt atttgtttat tgttgttatt ttttacaagt gttggtgctt
    11221 caatgtttta ttaaggtcag ttttgttgtt tatgttttat tatgtttaat ttaaatttat
    11281 tctattattt attttcggtg gcaaaatggt ggcaaatttg gttttgtcac catttatttt
    11341 tatttgtaaa aaatatagtt tatcaatata agtttattga aaaatatgga atatttattg
    11401 actttcacgt cacgtgatga tataatataa atatagaaag gaggtaagaa aataaaaaac
    11461 ttagaaggga ggacaaagaa agtggggaga ttagaaagaa gaaaaaataa aagagaaaac
    11521 aaatttaata aaattaaaaa tgctttctct ttcacattag ttttaattaa tctagtactc
    11581 gcaatactaa gattgattaa agaattataa gtttcaatcc tagaaggaaa ataccttcct
    11641 tctaggtagc ttctttctaa tattataaca cattttctaa tttaatatga ataataaatt
    11701 ttatagaatt tgtatgatta ttcttggtat cactatagtt ttaaatatta ttaatataat
    11761 aataagtttt acttttaaag ctcttattgc tttggtattt actttattat tgttggcatt
    11821 tattaatagt agaaaggaat gatattattg gaacaaagaa aacttagagt tgcattcaat
    11881 aaaagtggca aaggttcttt tacacctaga ttgatacttc caatgtcatg gattaaagag
    11941 atgaatattt ctcctgatga aagggatgtt cttgttactt ttgaggatgg aaaaataatt
    12001 attgaaaaaa ctgaaaatga ataaaaaaaa gaggtagtaa ctatttccag ttactacctc
    12061 ctaatcgatt tacttgttta taaaatctaa tgccttataa agagtgtcaa atctatcatt
    12121 accttttatc atggtataat tttctttagt catggaacct atcttctcac atgctccacc
    12181 accaacaaca tataaatttt ctgtctgacc tggtatgtaa tcttttatat cacatattag
    12241 tattttacca tcattatatc cccagccaac tacagttgca ggtattttgt caactgctcc
    12301 atcataaacg attgtatgtt tgtacatgat ttttccctca ccattctctt tattatctat
    12361 tgtcttattt aaaatacctt ctgctattaa tttagcaact atgtctttat gtctaatata
    12421 ataatctgta tctgctttac tatctacgaa gcacacttct attaatattg caggagcttt
    12481 tgtatgacta agccagtaaa gatctcttac atccgatttt gcacccctat ttttaaatat
    12541 tgttgatagt tttttgttaa ctctttcagc atatacctta ccattgttag ttttgtatat
    12601 tgtctctgta cccataggat ttagagttgt tttatttgcg ttgaaatgga tttgtactgc
    12661 taagtctaca ttttgcctat tggcaatttc acattgttct gctaaatagt tattagattt
    12721 atctactttt ccagtataca caatagctcc accttgtttc aaccatttaa caattaaatc
    12781 agttaaaatt ctattttctt ttccttcatc tatataacca actgcccctg ttccttttcc
    12841 tgttagtgta tgtcctggta ctattactac tttcatcatt tatcaccttc tttctgtagc
    12901 atcaccttta tatctgttac atcttccttt atttcctcca catcagtttt cattgctccc
    12961 atttcaacaa gtatattttt attgatttct tgttgttgtg ttgatagttc aataaagttt
    13021 tctactgtct ttttatacat gtctctatct tcttttttct cttgcatagt atttttgaat
    13081 aataaagcac ataagattcc tattgctcct aaactactta attctgttaa taattgttcc
    13141 atgatgacct cctactaaaa ttgatataaa aaaagaacct tttatacttt atataatagg
    13201 ttctattggt gtatcttctt tgtttaataa atttgttaac tctgtatact cactttcttt
    13261 tattctgttc atagcgtaaa atacatctaa cttagtctgc aactcttctt tagtttcata
    13321 atttttttgt tcaatcattc tttttaaaat attatacatg ttgttccctc cgttttttat
    13381 agttttataa cacgttattt gtgttaagtt ctaatatagc aattctataa gcattatcta
    13441 ttaatagatt gtcttgctcc ttttgatttt cttctaaaat tgtacctcta tcaagtgatt
    13501 gttctggttt gactggattg atttgtaatt ctaaccactc attataagac tgttcacctg
    13561 ttttaataat ttctcctttg cttattaaag gtgtccacaa ttgtccatta tctgcaataa
    13621 aaaaattatc aattcctata ttatcaattt cttcttttga aaaattatgt tctcttatta
    13681 ttctaaactt tattttattc atttttaaac ctccaaattt acaataacac taatcaaaat
    13741 tcccttgtct gtaatatcat gacctggagt taacatcagt atagtattat ttacacaagc
    13801 tatacctaca gtatcaactt gttcacaaga agaattcata actggacaag aatagttttg
    13861 tcttgatatt cctaaaggta atttaattat tgcttcttct aaaacagtag aagatgtact
    13921 ctttgtaaca atcatgttta caaataagta ttttcctacc tttgtaatag tagctgattg
    13981 ccatacttta tcccatccat ttatgaattt aaagtcaaca ttactttcag ttaatgttgt
    14041 aatgtctttt aaattttctc taataacttc tatatggcta cctaaatttc gtgtactttc
    14101 aaaactaaca tttggtatcg taatacctga gctacaaata aatcccgttt gattatcata
    14161 ttgctctaaa tttagtagac caagttcaac ttctcttggt atttctaatt cataaattat
    14221 ttttaaagga tttttcttta atttttcttt aaaattagca atgctaaaag gagatattga
    14281 ggtatctact atatctatta cacctattcc attttctgtt gatatagaca atgctgattc
    14341 ggtctgaaca ttaaatatat aatctacatg agattgcttg tataagttag ttataaactt
    14401 agacatatct aatcttaata cagatatatc taaatttaat ctaaactcat acattccatg
    14461 cacttctatc cagttttcat taccattaaa aactatttct tcacatcttt ttattagttt
    14521 ataggaacca tttttgtaaa ctatttcatc tcttattcca tttgctaaac ctcttaatgt
    14581 atgtggaatt gtctttttat catatttatt tcctttagtt ataattgttt tattcccatt
    14641 atcatgtaaa tcttttatta tagtaactct tagatacttt gcatttttag gacttctagc
    14701 aacataaaaa atatctttat ctcctattat tctttcattt aatggtgctg gtatgaaact
    14761 tttatttata tcataccaac aaatatttct actacaatta taaaaagtat actctgtatc
    14821 tggttctatt tctatatagt ctagggtata tttatgatta ctagcttcag aaataagttc
    14881 ctctccatta agatattgta aaatataatt atctttaaaa tcggcattct tattaaagat
    14941 attaattcca ctatattgat aacttaaaaa ttctatttta tctctttgcc caacacttaa
    15001 tagttcatta gagtaatcta tattaatatc tgtataatct ccttctaata tggtaatttt
    15061 caaacttact tcataaccag atgtaagtgt atcgttatct aaaacactcc ttaaaacagc
    15121 attacaatca cttaagtctg atattgttgt aaatgtatat ttaaatttac caattactcc
    15181 tcctggtata gctatatttg tcagattagg aataaatact gaattttctt cgcctaaact
    15241 atgaatatat attccacttg tactcggtaa agtgttttta tcaacatcaa ctatgattgt
    15301 atatatggta tctggtttat atgaagtgta attgattgta aaaaaattag aatatcttat
    15361 atcattttct gttagtatat ttatttttcc atctacaaaa gttgctttcc ataaagaaaa
    15421 atctgaacta gtttttcccc ataaatcaat taatgtcttt ccttctatat taaaattagt
    15481 taaatagccc tttttgctat tttctattat atattctcct gtatcagtac tacattttat
    15541 attgtcaatt tctttgagat tttctatatc atcaatttta ttttctagaa cttttaataa
    15601 agcttctatc tttacagatg aataagtagt tgtttcagta cttctattat catcaatagt
    15661 tgctgaaatc atgttaatag atgagtttcc atttacaact atacattcca attcattgtt
    15721 taattcatta aaaactttta tactattatc atctaaaatt tggtatgagc aaaatacatt
    15781 gttattagta gatttatcta ttaaacttac aatcactttt ctagttacaa ggttatgctc
    15841 aactatagct gtgtatacat tttcttctaa tatccagtta tttattgata tatctttaga
    15901 tacagataca ttaggacctc cctgaattaa tttttctaaa tcttctttat ttgctttctt
    15961 actaagttct aggttaatat tatcctgctc attatttaat ttattaatat tttttactac
    16021 atcttcattt aacaattaat caccttcttc taataaaagt tattttgtac tatttagcat
    16081 aagccatgca tccccacgct ccagaatctg aactctctgt aaacttacat cctcctacac
    16141 atatatattg tgcagttgct cctgttcctt gatgaatagg tcctgttgtt gttgttcttg
    16201 ttgcaacttg tgaaccagtt tgtaaatgac ttattgttcc tgttgtatta gtgccattac
    16261 tccatgaacc tattgttaaa catataaata tacattgatt tggtgttgta ctacttacca
    16321 tgccccctgt atcatttgaa tttctactac cactatctat tacagcatta gagccttttg
    16381 caaaatatgt agtattttga gtagaagtgc taatattact ataattagtt tgtaagtagc
    16441 ttgaatttgc tagactagtt cttccaattt cagttttaag tattttatta agcgcaagcg
    16501 ggcttttaga aatagataac atggctgtcg agctacttgc gatggcagac atggctgtcg
    16561 agctagagat tactatattt aagaatcctg aattatttat tattgtgttt actgctattt
    16621 gtgagcttgc cacagcattc atgtatttat cacttctagc taacgcatcc gaagtgttga
    16681 tatttatatt gtctatcccc gctagcttcg ctgcggtttt tgctatagct aaatcaattt
    16741 ccttgataga attgaaagct acttcgtcac ttgataaagc tgttataaca cttaaattat
    16801 tttctatgca ttgaaatata gcaattatat tatttttgtc taagttatta tttgataata
    16861 aatcttttat acttataaaa ttagtattca tattttcaac tataggaaaa atcttgtctt
    16921 gatattctat gttagttgaa ttaaccatat tagttataat tgttattaaa ggattcatat
    16981 ctaacttaaa tatactatta tgagttttta aaatctttgt aaatacaagt ctaataacat
    17041 ttgtatttgc taaaactaaa tctaatgtat cttgattagc taatagtagc tgtgtagctt
    17101 ctaaattctc aaatatcatt ctaactgatt catgatttgt tgatatatga gtcattgctt
    17161 cagtactatt tattatagct tctctaatta cattattttc taaaatcgct tttataacag
    17221 ctggttctgc tgtaactttt aaaatgtatt gtcctagcaa atccattgcg tttgaacttg
    17281 ctacaactgc gtccatactt gttttacaca tagttaaagc tactattgca ttactattat
    17341 taagtattgc attagtaact tccacatttg ctaatatttc ttctatagtt gtaagatttt
    17401 ctaaatcagt attatctaag ctaaaagtag aattaaacca tgctcctata gttgactttt
    17461 cttgttccct tgtaacatat ctatcatacc ctgctttaaa tttttcctct aatgaattat
    17521 aatgctctac agtcattcct tttactacat tatcagcatt tcccaaacta aatatatctg
    17581 ttctgtaaaa ggcatttaat tgttctccca gcttagtacc tttatcaaac agattatcaa
    17641 tgtctgtttc tgaaaaagct gtatacacaa cttctctaat tcctctatca gaaaataaat
    17701 ataaactatt taagtacatc tcttcccaaa tcctatgatt accttcatat aaaagataac
    17761 tttcagaacc atttaacttc aaatcttgta aataatttaa aaaatttaaa ggttctcctt
    17821 gaatatcact atttatttta taaacctctg cccaactcac taaatcaccc cttcatttgt
    17881 aacagtttct tctatcattc catttttaaa gttagttatt gttgtattta ttaatatatc
    17941 atttatatac ttttcttttg atatagaacc atttgtatta aactttgtaa tatacttgat
    18001 attattatct aatatttcta ttatagaacc atttgtatta aattttgttt ctttattttt
    18061 ataagtatct aattgagaaa gtttttcttt ttcttcagtt gtataatctt ctgtagagag
    18121 ttctttgcct tcaactttct caacatatat atcatgagta tgttctaaat tggctttttt
    18181 atctaatcca tcttttaata atctttttgt ctctatacta gaataagcac tttcaagaga
    18241 ctctatatca tcattaatag taaattttat aagttcaata gcagaaccac cattaactac
    18301 tgtaacttca atgtctatag caacttcatt aaatagtact atattattat catctataat
    18361 tttgtaagat gtaatcaaac ttttgttatt aagtttatct attaaagaga ctaatatttt
    18421 ttcagtaaca aggttatgtt ctactgaaag ttcaaatcct ccatcaactt caatccattc
    18481 agatgtatca tatgatttat taaatgctac atttattcca ccagcaataa gttgctctat
    18541 ttttaaatct agtttattaa aatcataagt taatctctct tttaaagtgt cttttatctg
    18601 accatcagtt gtttcttttg attgtaatat ttctcctgct gtatctaaat tttctaattc
    18661 attgaacctt ttttcaaact cagttatttt attgctaaca gttgttgtca tatctgtctt
    18721 agcagtattt acttcaacta ttttgttatc aacctcatct actttatcat ctactttttt
    18781 ctgcatatta attatagatg tctgcctagt tgattcattt agttctctag ttccctcatt
    18841 actttctcta agctcttcat tctcttctcg tttagtctct gaattttgtc ttattatttc
    18901 atttgttttt cttgtttctt catttgtttc ccttgttgtt tcactaactt ttctagcttc
    18961 ttcattggta tttctggttg tttcactagt ttttcttatt tcttcacttg cttttctagc
    19021 ttcttcatta gtatttcttg tttcttcaat agtgtttctt acttcttcac ttgcttttct
    19081 agtattttca ttactttttc ttatttcttc attttcttct cttgtttctt cattactttt
    19141 tctaaggtct tcgttttctt ctcttgtagt ttctgattct tgtctagcta cttcattaac
    19201 aactcttgtt tcttcgctac catctcttat ctcttcactt gcttttctag tattttcatt
    19261 tgattctctt gtttcttcac tagtttttct tatttcctca tttgattctc taacagtctc
    19321 attttcttct cttgtttctt cattagtatt tctagtttct tctcttgact ctctgtctac
    19381 ctcatttgtt tttcttattt cttcattagc ttctcttgtt atttcacttg tctttcttat
    19441 ttcctcatta gcttctcttg ttgtttcact tgtttttctt gtttcttcat tagcttctct
    19501 agtaacttcc tgtgtttttc ttacttcttc actctcacat ctttcaactt cactatcttt
    19561 tctctgattt tcgctttcaa ttcttacatt ctcattgcca attctaacat tttcagaact
    19621 tattctaatt tcttcattag attttcttac attttcattt tcttgtcttt cttcttcata
    19681 acttaataca tttaatatgt cctttattat atcccaacta ggctcatcct ctattgcatt
    19741 tctatttata cttttttcaa cttttattat tgtagaaaac gtagtaacta ccttatcgtt
    19801 tttatataaa gttatctcag aaataacatc tccttcttct gaaagctcta tatgacttaa
    19861 ttcagtataa acagttgaat cttctatagt acaatttttc tgagtaactc ttccacttgg
    19921 taaattaaaa aatgctaatg ctgtatatcc agttaaatca acatctatag agtcttgtac
    19981 tattctcatg ttataagata cattattatc attttgctta tatcttgcta tttgatacat
    20041 ctttgtgttg atatctacaa tataaattct atctctcaag ttatatcacc tgctttctaa
    20101 tacttctatt cgttttgtaa gttcttctat ttttttactt gttatatctt tataattttc
    20161 aaaatttgat atagtttttt gtaaagataa ttgtaatact gctaaatgag atgttatatc
    20221 ttgtgcatag acaccttcac cagcttcatc tgatacatct ttgaaaatgt acttagaaat
    20281 aggattatct aaagaggagt ctatatcaac ctgtagtcta tgttcaatat tgttgttttt
    20341 catagatttt aaattatatg gtgtgaatac atcagattgt actaagctac ttttaacaaa
    20401 atctatacag ttatttgaaa tttcttcatt aaatgtactc ttcatcattc gagtagatgt
    20461 agctgctgtt cttgctagtt gatttaaatt attacatact aaataatccc aagctttcct
    20521 atttgaacca caattatacc aaccatctat ttgaggattt atatgttgtg tattcaaacc
    20581 tgtattaatg taactttttt catcttgata accaaattca aatacttcct ctccatcaag
    20641 ataaccacta attacatcat cagaaacata tatatatgaa taatcattat atttcatacg
    20701 cattgctttt cctataccta ctccaaactg agcagtagca tctaaagcac attttgcacc
    20761 attcccctca aatagtaata tgaatgggtt tgatgaacta gcacctattc tgtcacataa
    20821 aatagtacct gtgtctatat aatcagcatt tatatataat ctacttccac ttaaatatat
    20881 tccacaattt cgattccctg ttaatgcatc aaatactgct ttttggtcac tagttaaaac
    20941 actttcatag ttatttttat cacctataga taactcatta gctcttatat tcacttttcc
    21001 attactatct acttgtaacg ttgtatatcc atcattatct ctgacagtta aatttctacc
    21061 atttataaat tgaccttcta aagtaccagt ttttatataa ctagcattta gatataattc
    21121 attcccaacc atatagagtc cctttgcaag accattgtta gttagtttat taaatatctc
    21181 aagttgagtt atatcgtcta tttctccccc aataatagat tccaacgact ttccttttaa
    21241 tgtaaaggta gttgctccta tatgaacatt tccaaaacta tctatattta aagttgtttg
    21301 gctatttcca tcaactacaa ctaaattctt tgcctcaata tactgccctt ttacttttcc
    21361 tacatttatc ttatctgcat ctaaatctct tatcactgca tgacctatag ctgcttcttc
    21421 aaaatattca gctgcatcac ttaactttct agttgttgca ctaacttcat ttgaaaattc
    21481 attatagtta tcatgtgtat ttcctgcccg tactctataa taccaagttt gcataggctt
    21541 aacttcatgg agtaatgaac ttgcttgacc tacatatatc ctatttgtat atcctatagt
    21601 atctggttca aatcctttta tctgagatgc atatacttca tagttataat ataacttatt
    21661 atcaaatgtc caatctaact gaatcatact ccataaccct aatgctgtga catttgaagg
    21721 ctctggaagt gtatccggaa aattatcact ttcaattaca atattatcta cattattttg
    21781 aaccttatct attttttctt ctaaatcatc tattctttcg tctttaaaat gatttactaa
    21841 gtcaccaatt tcaaccgaat tgtatttctt taatactgga ttatattcag tttttactac
    21901 ccttgcagta gcatttatac ctaagttata gtctctaact attatctcat catccatatt
    21961 tacagtttca agcatcttat agcttttata atcttctgta gttgataaat ctacaaattc
    22021 tactttgtaa gtaactttag gtaaatcaac attattttca ataaaatagt ctttacattt
    22081 attccttaag ctttcttcat tcttaacatc atcatctgaa aaatcaactg caacaattct
    22141 ttgagttgga taattgttta tatacttgct ttctatatat ttttctggta gtgtaatttt
    22201 tttattttta ccactttttt tagttgcata tggatatatc ttagttataa cttcttgtgt
    22261 atcataagta gctgttagac ctattatatt cttcctatat gctagtaata cattgttact
    22321 ttctccaata ttatttaata aacttatatt gaaattatct ctttttagtt tagacttatt
    22381 attaaataat tcttgtatgt cagatatagc attatgtggg cttacacaag agatggaaaa
    22441 gtctttattt cctgttatat ctgaatatcc tacaaatcta ttttcttcag tacatgacct
    22501 aaatatttga ttaagagctt cttcacaggt aacattttta agttctaaat tttctataaa
    22561 attatttaat aaatcataac taatgtgttc tgctttaact aatatttttc catcaagttc
    22621 cttagaaata tagtaaatcc taaataattg atttttaagc cttggagaag catcagccat
    22681 aactatttta ttataatcaa tttcatcaaa taaaaaagag cccacataat atgtaaactc
    22741 taactcaaat agtccattta attcttctgt aacttttgtt tcaaatgcat cttttagaat
    22801 ccccaaacca ttatttttaa aatttgtttc atttgcttta tataatctaa gcaatctata
    22861 acaccaccca tctaggttct atctctattt tttctacatc tccagagtat gttattatat
    22921 tttcaccatg tttaaatgtt ggaaaatctc caaacatcct actattttca ttaactacac
    22981 catttgtatg aatatctatt ttatatgcat tcataagctc acaatcaata tatatatgcc
    23041 cttgaacttc tgtaagttct atcacctcat tatttatttc taatgttata tctccatttc
    23101 caaatacttt tataagagga tacgaagcca ttccagtgtt tattagctta gaatttgatt
    23161 catttattgt aattaaatta ttaactaatt ctttcttaag tggtttacat tcaaatgtaa
    23221 tcttaaaata tcctaaatct tttataattt cttctaaatc tagtttgtta atacaaactg
    23281 cttctctata atagtttggg tcagtactta taataagatt tttatatgaa aaatcagttt
    23341 gtaaccacat ctttatttca gatgctagta tattaatatc tttaccatct acatctatat
    23401 agcattctat ttcaagatta aaattactat aacaaccttc atctaaaata aggctcccat
    23461 tccttcctgg tatctctatt ctttcaaatc tacgttctgg cgatgcaagt tcattaatat
    23521 ctgtaattac aataccaaaa tctctactat ttatttcgcc atattgaaaa gaaactaatt
    23581 cattttcact gacacagtat tgtaaagcca aattatcacc tcctacacat aaaaaaagag
    23641 aggactcaaa tttttctttt gatacatctc cttctattta ctttgcatat ttttgctaag
    23701 atgtgatata ataaaagcaa gaagaactac aatctattgt cagtagagcg gagttcataa
    23761 ttaaaagcta attatttttt atggaatttg atttttaaat caaactccca gccactcttt
    23821 cgcacagagt ggctttttac ttttgcaaat atcttactta ttaagcaaaa tattaagcta
    23881 gcaataacac cagctattac attaagtaaa aagttgttca tacttaccac ctcctttcat
    23941 tagaaagtag gttttatctc agtatgaact ccactcttag attataggtt acatcttctt
    24001 gctaaaagta ttataccaca atttggatat taaaaagata ttatcacatt aaaactttag
    24061 ttttctattt gtaataaatg ctatttcaga acctaattgc tctatatctt gtttagtatt
    24121 atttataaat ttttctatat gtaaagttaa ctcaactttc ttatcactac ttttttcact
    24181 atcaacttta ctattattat ttatattaac agttgcatcc ttcagtaaat tatttgatgc
    24241 tattcctctt gccagagtat ctgaatatgc tatactttgt aaattttcta tttcatttct
    24301 aaaccttgct gcttcttttc ttcctcctgt aggtatattt acatctttaa atcctatatt
    24361 agaaagttgt ttatttatag aattaactat gtcacttaaa accttaccca atgctccttg
    24421 catagattta ataccatcta tcataccttg catagaatct tttcctgttt ccataaacat
    24481 agctggtact actccaaatt tatccttaaa actttcaatt tgttcctcta tacttctttg
    24541 catttcttga cctagttttc tatactctgc atcataagtt tttttcaatt cttcaatttc
    24601 tttttctatt ctatattttt catcttctgt atcttctttt gctcttcttt cagctatttt
    24661 tttcttttct tgatataatc tttcgtattc ttctagttgc tcttttgaca tcttgttaat
    24721 agcttctatt tcattatggg cttgtggtcc tttagctaat aattcctcat ataaatcttt
    24781 acccactttt gtagataaat ctgccatatc agtgtcccat tttctaagta catcaacttg
    24841 ctcttctaag ttttccatta actcatcatt gtcaatttcc tcaaatgtta cagaagaaaa
    24901 taaatctgta taatccatta aagattttac tcttgagtca tatatctttt gatattcttc
    24961 attgagtttc ttttggtcct caatatattt cttatctaag tttgctactt tttcagcata
    25021 atcttcttta atatcattat attttttatt gaaatctctt tcagctttta ttttctcttc
    25081 tctaagtttt tgaagttctt cttgatgctt ttcagtatct tctttttctt ttttatcata
    25141 ctttttatta attttttcca acttttctct gtattcttct tggtctttta cagatttctt
    25201 attatattta tctttaatct tctttagtgc tgctagtttt tcttttttat ttttatattt
    25261 ctttgcttct gcttccttta tttcttcatt ttctttttca tttctttctt tttgtttctt
    25321 cttatgttct ttatctaatt ctgctatttc ttcagctttt tcttcctgta gctttttaac
    25381 ttccttatta aactttttaa tttcttgtgc ttcttttata tttccattac ctattattgc
    25441 ttgaactgtt ctaagtgtat ttgttaatcc ttcttctatg tttgacaatc catctaaata
    25501 tgcttttgct gagttttctc ctgctttttt aaattgtgag acagagccag caaatacttt
    25561 agctgtgcca ttcatagtac tcataatttc atcaaaaata ctacctctca tgcttgtatt
    25621 attaaatgtt gacattttat ttataccagc tgcaagagaa tctttcattt taatattaga
    25681 ttcaataaac tcatcacttc taaatgaaaa agatactgac tttggcactg aagaaactgc
    25741 acttctcatt gcactttttt tactattaaa tccatctatc catgcttgac cagccccaga
    25801 accagcactt ttaaatttac ttttagaatt atttgcagct gttacagcat tactcataga
    25861 aatagccacc acttgtgcta atctagagaa tgatgtattt acaccattgt atgcatcaga
    25921 cccagcctgt ttagctactt gtgcaagttt tgtaaatgat tgtttagcac cattgtacat
    25981 cgctgtgctt gattgttgta tagattgatt agctttttta aaatctgcat ctgtagttgt
    26041 tgcaacctta cttgtaccac ttttagctcc acttgccatt ttgctaaagt tattagttgc
    26101 attagaagcc attgtagcag ttgatttatt tgcattagct tgtgttgtat ttaaatcttt
    26161 agtaacagaa gtctttaatc cactcacctt tgaagcggca gaacttacat tagcatctac
    26221 tttcaaagtt ccagtcacat tatttaactc ttttgtttta ttagacactt cctctagttt
    26281 ttctttacta tctcctgtat ttacatttgt gttcacgtta gcatttttag gcaatgcttc
    26341 tatttcttgt ttaacacttg ttacattttc cttaccactt acattagttt caatttttat
    26401 tcgttcttct tttggaagtg cattaagcaa catttgagtt gattcaacat cttctttccc
    26461 ctgtacatta cttaaaatat tagttataat ttctggtggt attttagcta aaatatcatt
    26521 tacattttga gcaccagtta gagcttccat attattagtt tttatataag tttgtacctc
    26581 tggtggcaac tgttgatata gattatataa ttttaatatt tcttcttcgc tttcagtacc
    26641 attatttttc agcgttgtaa caacttcttt aggtacttgg tcaaaagatt tagcaacttc
    26701 tgaaccagct ttatttgctt cttcttccat tgccttccaa tacgtactag attcttttct
    26761 cagattatct attaaagtag ctccatcaat acccattgaa ttgaagttgg atataattgc
    26821 atttttcatt tgttctgcac tcatagagcc atctgtcgca actcccttta atatatcact
    26881 ccaagtacca cccatagatg taagagctga tatagtctgt tcatccatac cttgcatgac
    26941 tccagcaaca ttttctgcca ttccttcaaa tccagcttga cctattgttt taaattgatt
    27001 aaatacatct gttgcatttt gttttaaaac attactacta tcttttacat tttcatttat
    27061 caatctcata gcattatcta tatcttgttg taatgtttct gtactgtatt taccacttat
    27121 aaccatgctt tctaaatttg acgcaaattt ttctgtagct gctgtagaat ccatgccttg
    27181 atatatccct tcaaataaca ctgccatact atctgatgtg cctctgagta tagcaattga
    27241 ttgattatca agcccttgca aattttcagc aaatgtactt gcaacttgac ctacattgtc
    27301 ccgtgtaagt ttaggccatt ccttcattat taagtcaaag gttgatgtaa ctgaacttaa
    27361 ttgacttgca gttgaatttt ttattttttc taaagcactt gaagtttcag ctcctatatt
    27421 cgacatagct tttgctgtat tattttctat attagcccat gtttttcccc aaatatcatc
    27481 aatttcccag accttcccac ttaagagagc tactataatt tctcctactc cttttagtag
    27541 aattaataaa ttaccaaagg tcatttgagt tattccagct acaaactcac atactgttcc
    27601 tagcacagtt ccaaacactc caaatttatc ttgtaaaaat tctaatgcat ttgcattatc
    27661 tcctatcata gttataagac ctgctattac tcctacaaga actgctatac ctgcaggact
    27721 tgctaaaatt gcggctccaa aacttacaat cgcacccttt gctaatccaa acaaagcaat
    27781 tatttttggt atatttatga ttaaagcact taaaacaacc aataagggac ctattgctgc
    27841 gactacagct cctatagaca caataattct cgctatttct ggattggctt gtatcatact
    27901 acttgcccag ttcataaata aagttattgc ttctgccact tttgatataa ttggtttcat
    27961 agcctctcct aaatcagata gcatattctt tgctgaattc agtgcatctt caagttttgt
    28021 ttttgtagta ttatccagtt gttcaaaagc cttatcacaa agaccagcag aatttttcat
    28081 atttcccagc atttgattaa atgaatcagc actctttgta ccttcgatta tttctcctgt
    28141 agtttcatta actgcatcac ctaacagaac attagccgcc tttgcacctt ctgctgaacc
    28201 aaataggtct gatagagata aactattttt ctttgcataa tcatttaata ttaataatac
    28261 atctgctgtt gattttcctt ctttttgcaa atcagcaaat cctttatctg ttaatttttt
    28321 aagtatttta tctgctttac tcccgctttt cccaagctca ttgaacattg cattcatgta
    28381 agttgttgct tctgcgccag caattccttt tgcagttaat atggcatatc cagctcctag
    28441 ttgctcaact gatacattca aactttttgc tgttggtatt attttaccca tagaacttgc
    28501 taactcatcc accgtaattt tgcctttatt ttgggtctgt ataagaacat cacttacatg
    28561 agccatatcc ttctgagaca ttccataggc attttgaaca gttgtcaaca catctgtagc
    28621 ttttgctaaa tcagttaatc ctgctgttgc tagtttatct gcttgtgcta agaattcggt
    28681 tacatctgct tgttttacac ttgcagatat agcattatat gcagcattag catattcatc
    28741 ataagccatc ccgatttcat tagcaccagc tttcagagta cttgaatagg catcccactc
    28801 actttttcca cccttaactt tttctgtatt tagttgtagt atagagttaa caactgacat
    28861 tgcttgttct gtgtttgtag ctgctgttac tgctgctgtt cccattgcaa ttagtccagt
    28921 tgtaactgta gaagttagtg tttgaccaat atctctcatt ttgccaccaa tagcatcaaa
    28981 tggcatactt cttaatgaat cactaagtct attagcttct gcctctgcat tattcatagc
    29041 tgttctcata tcagttaaag aatcttctcc attttcgatt tcattaccta attgagtttg
    29101 agaattttta agtctttcaa ttgcttctct atatctttgt gactgctctg aactttctcc
    29161 atacatactg ttacaacgtt ctaattgttg ttcatatctt tctattttgt ttcctatttc
    29221 tgtatacttt tgcttcgact tatctatttc ggatgtaagc cttgatattc cttgtccata
    29281 agtgtctatt gtacttcttg ctgttcttag ttcattttca gtttccttta tagaattttt
    29341 caaagatgaa aatggcccac ttgaagttga tgctatttta tttaattctg atgctgctaa
    29401 cgctgattgc ctacctattt gtgtcaatga gtttgctgta tcagaaattt gtttacccat
    29461 tgccgtcatt gcactacctg tggtttttgt acttgaatta aattgactaa gttgtttagc
    29521 tgcatctgca agagctttaa aaaacttccc tccatctaaa tctagataac ctactgctgt
    29581 tcctaattct aaggacatat ttttccctcc ctccctcaaa atttcatata aaaaaacacc
    29641 tactatttaa gtaagtgttt tctgtattat tattttcagt ccattatttt ttatcttcta
    29701 caagtttttc tatattgcta ttcaattgag ataattgtct aattatcatc cagttttgct
    29761 caactaaagc actaagataa tttattttag tttgttcttc tatttttgta aaactcaatg
    29821 ctaatcctgt ttttataagt ccagttgcaa ataaatcatc cgctattctt cttaaaatag
    29881 ttaaatcttt ttcatttaaa tcttgtaatt gatacttatt catgaatttt tgtatttctt
    29941 cttgctcttt taatttcttt ttatcttctt tatcaacttt atccccaaat aatgccataa
    30001 cattctcctt cactataaat taaatgtatt ttctatttca attatataat atattaataa
    30061 ttttttcatt aattatttgt aatattattc cataataatt tcacataact gattaaattt
    30121 aagcacaaca aaaacactta ctatttaaat aagtgttttc tgtatttgtt caaaatttaa
    30181 gtccacatag ttaatataat acctgctaat gctccaaatg ctaaagttaa tgctcccatc
    30241 tttacatact gcttagttaa tataatacat ggtgtaaaga catagattct aaattttcaa
    30301 cttggcttta cataccactt agttaatata atactttttt taggcaagtt atgggaaagt
    30361 attaaagaaa ctttacatac cacttagtta atataatacc tgttgtttct tgttgcattc
    30421 tagaatagtc actaacattt acataccact tagttaatat aatacattaa atttagaaca
    30481 ataaaattat atttctgcaa atttacatac cacatagtta atataatacc ccaaaataaa
    30541 ttgagcactt ccagttcttg cacatataaa accatattaa aattgcagtg agtaaccagt
    30601 agtgtttttc acacaccaca aaacatgctt atattgtagt aatttcaatg attacatcca
    30661 atttatataa aaaatcgtac actgcaaaat atctatatta tcattatatc ataaattaac
    30721 attaatttat tctattcatt tagaatattt tacctgttaa cttttttctt ttctctaata
    30781 ctatttttat tctaaagtag catttatttc tcccatgacc ttattattta ctttatcttt
    30841 tacaattatt tttacagcat ttgagtctga tgttatagca aacgctgttg ttccctttga
    30901 tttagtacct gctgttgttt cttgttgcat tctagttgaa tcgtctactg gataagtttg
    30961 tagcacattt ccatcaccat ctgccacttg atattcaatt ccctcagtta caaatacagc
    31021 ttctttacaa tctacatttt catagttaaa atcaacaaaa actacttttt tagcttcaat
    31081 ttcagcgaac tcattacgtt catctgtgct tctcacacct tcaaatgtta gcttgtaagt
    31141 tccttcatca tagtctttca tttctatagt atctccaaat ttatactctt ttttttcatc
    31201 tttcttttgc tctgtctttg catctttttt agatgaatca tctgacgaac ttggagaact
    31261 gcatccaact gcataaatcc caattactaa cgatagaact aatgataata tttttttgtt
    31321 tattttcata atataatccc ccagtgaagt tattttctca ttataaattt tacactaaat
    31381 cacagagtta gtctattata atagaataaa ttaatactat tttctatctt tttctttctt
    31441 ttttaatgcc atatcaacaa aagtagttct ttttatattt tctttagaag tattactatt
    31501 ttctttaaat ttaagctttt tatcattttt tatagcatct actaaaaaca tacaagcttc
    31561 atcgaaacaa aaagaagtat attcatcttt aattcttaat atctcactag gcatcttgtt
    31621 gtacattatc acttgattta gaatcctcaa tatctgtgga ctcttgatga aagttttcta
    31681 gtgactttgt acctccttga atacgattaa ataatgttaa tctttgttca atagaaagtt
    31741 ccaatcctat ttttttaatt tgagcatatg tagggttaac tagcacttct ttagccacta
    31801 tatccatcat ttcaaacatt tcttttgaga aattagactc atcattcatc ttctttagta
    31861 ctttttctcc tgtactatct ttactttcct tctcttcaac taaactcatt gatgctgtca
    31921 ttaggctgtt tggtatctta ccagccattg caagagccat taagtcaggt tgttttactt
    31981 ctgcaactaa catagttcca tctaaaaatc ttccaacctc tattacctca gtcaatttta
    32041 tttttttcaa ttcttctaaa cttgttactt taagttcatt catatttatg tcccctttct
    32101 aaaaaaagct ctagctaatt actagagctt taatttatct atttatatac ttacactaac
    32161 ttcttcaacc ttatatgttt ctccatttgt taatcctatt atctctgttc ctgttaatgc
    32221 tgctttatca gactcagtat ctgttaatgt tccatctgct aaagtatatt ttatattgct
    32281 attaactgta accttatata ttttgcctgc tgttaatcct gttatctttt tatctcctgc
    32341 tgttgctcct gatacagatg cactgtctaa aaatactttt gtagcttcat aaccattagg
    32401 taattcctct actatttcaa cctcataagg agattgccct gtgttaggtc tactatttat
    32461 aacatattca tttgaataat attctccatc tttaaagttt agtggaacag attttccttt
    32521 acaatttggg aaagtcgtct tagcaaattg cccagtatct cctcctggtc caacaactga
    32581 actataaatt attgttttaa aagatttctt agaaaaagtc ttccctactt ctggagctaa
    32641 atatctctta aagctaccat cttcctcttt ttctattgtt ccaccttgca ttatttgaag
    32701 tatttcagga caaaatacat tatcttttaa tgtcaaatca tatcctaaac ttgtatcttc
    32761 tgctgctcta tttgctatta tttttttctt tatttttaaa gttaattctt caccttcaga
    32821 aatgatttcc tctgtcccta tctcatcaga tgtatcaaat gtatatgtaa ccggattttg
    32881 ttcaactgtt tcaatttgaa ctaatacaac atctgtaagt gggtattgat ttaatatttg
    32941 tactgccata tttattacct cctaatttgt ttgttttcat aataacttaa tctcgtgaaa
    33001 taagcttgtt tttcatcatc tataagaata ggtaaagctt cataagcttg ttttattcct
    33061 actatttcat ccatgacact ttcaacactt atcttgtatt cacttacttt agaatactgt
    33121 ccaattggat aaaatacata tatttcaaat aaatctttta ttatattttt ttctgttgtt
    33181 tgtagaggac ctttttcaaa tataactaaa aaaggttcta tgcataatcc ttgatgttgt
    33241 ccaatagaat acacattaaa acccttattt tttaaatgtt tatatatttt tttaaacata
    33301 cttcacctac tttaaaatca catcaagacc tcttataata cttggtgcac atttttctat
    33361 agttggcatt ataattggat atggtctact tcctggatga ttaacttttt taacaggatg
    33421 tgatgctcca tcccaaaata aataacttcc aggtcttgct tctataacat gtggacctgt
    33481 acctttttct aagtataatc catagtcaac tccatgacta agtgctatcc ttatagtgtc
    33541 tccttgccat tcccaactac catgtagcct atttttagca tcatgagtat ggtctttcca
    33601 tggtctatcg tttttggcag tgctttctaa tagttgagat gaagtgtctg cataagctcc
    33661 tagtgcagcc ttagttttca tttctctact tactaaagca ttaagcagct tgctagtatc
    33721 aaatttaaat ccattactca tactatctca cctttcctaa ctttaaatca aaataaatat
    33781 tcattctatt ttgatttcca aggtcttgta taataaactt attatcatct aaatacaaaa
    33841 aatcaccttg ctttatcttt actgtatctt catcacaaac aaccattaaa tactgctctt
    33901 tatcttttat aacaacacct ttatcctttg ttatttgact tatagagcta cttccttcat
    33961 gatataaacc tatcatatta caaactatat tttcttcatc tgaaggctct ccaaactcat
    34021 ttactccaac tctttttact gtagcttctg ttggcatctt attgatagct ttgattatct
    34081 ttggttttac cttttgagct atcatagaca tctacttcca tttgttttaa acttttttgc
    34141 cagagttaac caatatagtt tattttctgg catgcttaag cctccaggta aagtgatact
    34201 atcattttca gcttttaata tacatagttc atacatagtt ttattaaaat cttgattgtt
    34261 tttattgtag taataagtaa tttcatcatc agaaaaaaaa ggtgattgtt cttctctaag
    34321 ttctgttttt atatttttta agatttctaa atccatacta tcacctacta aaaactatat
    34381 tttataactt atgcttaaat tcaactattc ttatttgctt aggctcataa actcttgtcc
    34441 agttttcacc ttttgctaac tctaatcttg aaggaccttc tgttttagct acacttgcat
    34501 tagtaaattt tatacctctt ggatgtagta tatatgtttt tctattgatt aaataatcta
    34561 ctccagaacc ttttttctta tctctatcta tttctgtagc aacaaaccct actggattac
    34621 catttcctaa agctattgcc ccactaccaa atagataaga agtataaact ccttctttag
    34681 atacaggaca accatcatca actataacta atttatcttg ataaacttca aaatctggac
    34741 ctacatctgg tctaatagtt tctattaaat tttgtttttt aagtgctgat tttgtagctg
    34801 aatgcattgc tatagccttt aactgacctt gtgcatctcc taataactgt tgtgcatcta
    34861 taaaagctga tggtgaccat tttgctgcat ttcctgttcc tgctgatata tctaacttat
    34921 ttgttgtcat atttgcagct aaaaatacac catttaaaac tgctataagt tctttttgca
    34981 tatctctagc ccagaagctt ccaactaaac tagctattgc tgccattggg tcttttcctg
    35041 ccatagctgc tgataaatca gtagctgccc acatttttgc tcttcttaat atagctgctg
    35101 catcttgact tgaagtaatt ttatttggtg ttaaatcagc atcttctatt atttgctctg
    35161 attctccact taaatcctca aagaaaggca tatttacaac tggtgcagct tgagaagcta
    35221 aagcatttaa gcttgcatca tttgtaatta ttccactttg atatagagat gataactcca
    35281 tgcttttgtt tattacatat gggttaaaca attctggtac tattacatca cttaattttg
    35341 ttactgccat ttaaatcact ctcttcctta taaatttatt cctgcttgag ctgcaaattc
    35401 tttagcttta tcagggtttt cttttaataa tcttccttgt tctgttaaat tgaatgtttc
    35461 ttttataaaa ggattattga ctacatttga ttttcttggg aaattccctg gagaacctgt
    35521 attacttgga gtagaacttt caaaccattc tttatatgtt tctgatatgt ctttaaattg
    35581 ttcatctaat cctataattt ctccagtatc ttttacttcc attttactgg tatcaaattc
    35641 tttacatagg agcttcctat atttttcatt tactccagat agtttagaat ttatagcata
    35701 gtctatagtc atattcttta ttttagctct actcgtacta tcaagttcat tatatttatt
    35761 ttcccattct ttcacttttg cctctatatc ttcactgttt ttactatttt tcttcaaatc
    35821 tgatatagtg tcatttgcac cttttatttg ctcttctaag gctaattttt gttctttcaa
    35881 cttattgtat ctttcatcta tattttcttc cttagaagtg tagattttct cttttttcat
    35941 accctcaatt atatttttta tgtctgtgtc ttggtacccc aattttttta ataattcttc
    36001 cataattccc tcctacacta cgcttttata cgagttttgc ctctctagta tagtttgcta
    36061 tttatttctt ttacgcctat atatagctaa aaaaggcaat aaaaaaacac ctactaattt
    36121 ttaatttcag taagtgcctt aatcttcttt tattactcca atttctttta atttctttct
    36181 aaattttgtt cttatttgtg cttgttcctc ttcagttcgc tctacttccc taatagtctc
    36241 cccccacgga atgtctttcc atcttggatg gctgaaaggt tctaagtact tatcatcttc
    36301 aaatttatta cacattttat agctcctctg actattataa atatttactc ttccattaca
    36361 cctattttct tgagatgttc tctaaacttc tttttgttct taatcctatc ttcttctgag
    36421 atttcttcat gacctatcac ttcaaatcca attggtgaat attgtgttct ccataaaaac
    36481 atatcatgag gagataattc tttataagca tctccttttt cttcatcagg tagctttata
    36541 aattcttcta ttgttagcat ttaatacact tcctctaata aaatatgaat tgttccctta
    36601 attctttcaa tttcaataac tttaaacatt gaacctcgtt tatataatac ttcttgttct
    36661 ttagggttat atttacttac atcttttcct ttttgtgaat ttaatatata catctgcact
    36721 tgaccctcag aattataagt acttcctttt gttgttgaag tatagtcata attagtaact
    36781 atctccccca acttgtacat acttacgaat ctttaattta agtaagtgtc tttaatcttc
    36841 ttttaatact ccaatatctt ttagatgttc tctaagtatt tttttatatt tttttctttc
    36901 ttcttctgga atgtctctat atcctatagt ttcaccccat ggtacatctt tccatcttgg
    36961 atgactaaat ggttctgaat acttatcatc ttcaaattta ttatccattg cgttcctcca
    37021 ttaatatata atatttgcca ttcatttctt ctaactcaat tatttcaaat ttagaatttc
    37081 ttctatacaa aacttcttct tctttttcat tgtacatact tatattttta ccattcttag
    37141 aattgaatat atatatttct acttgaccct ctggattgta ggtttttcct tttgtagtcg
    37201 agataaattc tgagtattgt atagtttttc caatttcatg ttcaattaaa aaagcttcta
    37261 aactttcttt attataaaaa tataatgacc ttgtaacatc cccttcataa tttggcattt
    37321 tatctaaaac tctatctaag ttatttataa accattcatc ttcttctgtc aatttaatat
    37381 ttcttcttaa tttctcatta attttatagg catctccacc catatattta tataaagcta
    37441 acatttcatc ttcagttggt tctttattct tattagcttt tccttctatt cctgcaaagt
    37501 ctaatccata ttcatcaaac cattcatcaa gtttagagtt ttcttctcca tctatccagc
    37561 ttcttaactc tttacctatg tcctccatac tcattggaat atcatattca aaagtacact
    37621 ttcctaaagg atgctctaat ggtaactctt ctggaatata tgtttttcca tttctatctg
    37681 cacaaacctt acacatccta ggatgatgtg atgacatcca tttaatccct ttaacaaatg
    37741 gattcttctt acaactttct ttagctgtct tttgatatgc atgagttata tatgtagatg
    37801 ccagtctata ggcattaaaa tctatttttt tgttactttt atggtatacc tttgaccaat
    37861 cataatcttt cttaaccttt ggattaacat acttttctaa atcttttgct atatcataac
    37921 tacctctttt ttctgctaat cctctactta caatgtagtc taaatccttc ttagttttat
    37981 ctatattact ccaaattcta tcacttaatt taagtttgtc tttgtacatg ctaccagata
    38041 taacttgcct tagtacatct tcatgaactt tactaaacat atcagtgaat tgtggcttta
    38101 aattaactga atagttatca catatgttat tgaaaaaact taattgttca tctgtagtag
    38161 tttttattac ttttgatatt tcactttcaa tatctttttt tagcttcttt cctaacctat
    38221 catactcttt atttaaataa attatacttt ctcttaaata ttgttcagtt aatgtgttag
    38281 agttaactct atttaatttt tttgcatatt cattagctat atctttatat aactttctta
    38341 tctttttagt agttctattt gatgatatgt ctctagctct ttcagcattc ttcatagcct
    38401 tattaaactt attagccatt attcatcatc aactacttct tttgattctt tttcattagt
    38461 tacttcgtct gattcttctg tatctatagc ttctgtttcc tcttccaaat taaaactttc
    38521 ttctaatatt tgtctttcaa ttgatatttg ttgcagttct tcatctgcta tatcatcatt
    38581 actattattc cacttcttaa tgaaagtctt cctagacatt gcttgagcat ttacttgttg
    38641 aatatcaagt aatttttctg aatcctcatc ttcttgtaat ggatattgat tttcaacaat
    38701 tacttcataa gaatctttgt ctaatatagg tatttttact atattatata cttctatcat
    38761 ctctatcatg gcttgtatca accattcaag agctggaccc catgatttca ttttttcctc
    38821 acatctagtt attaattgcc aatacaaagc tttcattgat ttacctgatg tcatcatacc
    38881 tttaaggtcc tgattattta taagaggtat atttagtact tcatgcatat ctgatttaat
    38941 tctattaaga gagttttcta ttctagtatc atatccaaag tcagtaggaa ttgtattaat
    39001 ctgtgcttgt ttttggtcag ccgtttgaga agtttctaca tcccaataag ctcctggttt
    39061 tattttaaaa tgcttacttg actcttcttc aacatcaaca ccatatatga tacgattcat
    39121 tcctttttta agagtatcta tatcctcaga agctagctta ttataagcca tctgattatc
    39181 aaatatctct tctatgtcac tctcaccctt taaatcacca agcaaaccat cattaagaat
    39241 tacataacaa ggtattccac taagttttaa atctgtatca ctcactattt cttctgtaca
    39301 taacccataa ccattataaa taccttcact taatatacat ttatcattaa ccatttcata
    39361 tttttgtttc caaattctct gtttatcttt ttcaacttct tgatttgttt gatggaaaaa
    39421 tacgattttc ttgagctcat caaattggtt atcaaatggt tcatatataa actctaaact
    39481 tggtacaaac attatcttta aactcttact ttctttatct gcatgtagct ttatagctat
    39541 tcttttacca ataaaacaat ctctagccgc tttaattagt ttatctgaaa acaaattttt
    39601 ctttagaatc ttattaatac actggtttat ttcttctgcc ttatctttat ctaaatcatt
    39661 ctctggtttt attgtaaata ttggtgtctt accaaataaa aatctagctt cttctttaat
    39721 cagcttttta atataatttg ttttcttcct tgtttgttta taatcttgtt cctcttctat
    39781 ccaatgttgt cctgtacctt catacttatc atatagcctt ataatttcac ccatttctct
    39841 tattacttct tgaccataca atcctgtaag ttccatctga attatatcta tcaaatctaa
    39901 catgttacac cccctatcta ctgttatatt ttctttctct tccatgtgtt ttcatatcta
    39961 actctaatgc atatcttgtt gcatcaattg aatgattgtc tttatcttct aacttagctc
    40021 ttacatttcc atccttatct gtttgataat ctatattttc aaattctcta gctatatttg
    40081 gagttctgtt tgggtctatt actattgctt gtaaatcatc cagccagttc tctccaaatt
    40141 cgattgaacc aggtcctttt ttagccttca atgcttttat tccatattct ctaagctctg
    40201 caatacttct tggttctgca ctgtcacaaa taacactaaa gtcatcatat ccttttgatt
    40261 taatcttaga agctaattct cttatagaca ttttcacccc aaatatttca tctataaaat
    40321 atataactct tttctttttg tcataatgca gtcttacaaa tgccataggg tctgttgcat
    40381 atccaaagtc attaccttgc cttatgttgt caaactggaa tatttcttca tttgtgattg
    40441 ttttaaactc taaattagaa aaagggacta ctccagaacc aattggctct cctaagtact
    40501 cccatctata tttaaactca tttcttattt taatttcatt agcttcttca atgaaagcct
    40561 ttgatatatg aggattatct aaataaatac tatgatgaac atatgtattt ttaggtaata
    40621 catgtatttc aaatttctta ttaacccatg attgttttct ttttggagga ttgtatgata
    40681 ggaatatttt ataattcaat ttgtctggta attctccacg taacactgaa ttaattacca
    40741 tagataactc atcctctgtt tttatttctg caacctcttc aaaccatgca aaagctattg
    40801 gatacttagc tgattttata gattttatct tttgtgggtc atcaagccct ctaaatataa
    40861 acttatttcc tcttggaata aaaataattt ccattgggga ttttttaaac tgaaataaat
    40921 gtgttaaacc aaatatttct atagtttctt tcaattgctc atatacacta tccattattg
    40981 tattaccaac tttcctaaaa caaacagtat ttactggata tttaaccata gccatagtta
    41041 accaaaaggc tatatgtgtt gacttagctg aagctcttcc accttttaat acatgatata
    41101 ggtatttgtt actattactt actttccaaa attcataaaa atttttattt attatttcag
    41161 atatttttct atccatcatt taaattacct acatcatcaa ttatagttac accaacattt
    41221 ccatctaaat caactttttc agtccataaa gcataccttt tacccaataa ttcagctgcc
    41281 ttatttctat cttttatact tacatctttt tttattattt caggaccatt ttctgatact
    41341 attacaactt cttcttgttc ttgatttctt agtatctttg ttaaatactc cataacttct
    41401 tttgcatctg ctatcctctt tgattcaatt tgctttaatc tttcatcaat gtaattttta
    41461 accttatcat tactcagcag tctacttcca ttactttctg atgttctttg attgttattt
    41521 ttgtatgctt tcttgtatgc ttctgttgca ttaccagttt caatataata atcacagaac
    41581 cttttttgtt tttcagtcaa atctgccata ccaccacctc tttatttgct ttataaataa
    41641 aaaaataaga ctttaaattt aatctaaagc cttattctta ggggatacat attatattta
    41701 agggagcaag ttttaggaat cgaacctaag attacacacc agtccttgca aattgagtga
    41761 ggttaccaag ccccactctt ttagacattt gaattaaatt ccgttttaat ccagcatatc
    41821 tacatatagt gtattaataa gtttgaacat agtaagaatt gaacttacag catcctcatg
    41881 ccctgcctag tctgttcata taagctaggt gaatcccttt acctagccca catatattta
    41941 gttttgagag agaaatattc atttccacaa tactattatc tcacacttta aattgtaaaa
    42001 tcggcagaaa aacggcaata aaaagaccta gaatttaatc taggtctttc tttttaatcc
    42061 taatttcatt attttcaaaa taaacttcaa cttctctctc ttcttgtgtt atattcattt
    42121 ctttaatcca tgttgcaggt agacttattt taggacttag tgaaccagag ccactttttg
    42181 ataagagtac tttcaatatt cttttttcca tttctttctc tccctctagg aaaataaata
    42241 ttttatagca aatattaata atgctattgc accaattaat ttaactatac caaaacttaa
    42301 tttccctaat tctgtaatta attccttata ttttcccatt gtattttgag tggtcatgta
    42361 ttattattta attagaaggg aaggtgctga ctccccttct aaagtcttat aggctatcta
    42421 ataccatttt tataacggct attagtgtac ctatttcaag agcaagttcg gtaagggctt
    42481 tgacaacttt gccgaactct tttatttttt tgaccacttc tttcacctcc tttctatatt
    42541 tatattatat catgtcgtac cgatataatc aatacctttt tccctttttt tagtaaaaaa
    42601 aatagacagc tattcactgc ctataaatct aacatcttaa ataatggttc ttgctctatt
    42661 agtgctttct ttccaaacaa ggctattgat attgaactaa tggcttgatt agctctttct
    42721 cttaattgtc tttcttctaa gtatacttta tcaactatta aactccattc taagccttca
    42781 atatacctat accttataat ttgtttatgt ataggtttta aattgcttat ggatacatct
    42841 attgtatatt ttagtgcttc catttcatat aattctatct gcttttctat tatcttttct
    42901 tcaagattaa ttaactcatt ttcaacttga ttacttattg aattagtctt acttatggga
    42961 atgctgtcat agcttaaacc ttgcataaaa tcacctaaat ggaactcttt aagatttttt
    43021 atttgaagtt ttagactttc aatattaata tgtagttgtt tgtagttctc aaggtgtttt
    43081 ttagttgcca taaaaaactc ctttttaact ttacttgcca taacatcact cctatttatt
    43141 taagctacct ttttcttatt ttcttttctc tttttcttaa gttcactata ttctatccaa
    43201 ccgtctaccc catatttttt gcttttagca atccatatca attttttgtc ctgatattta
    43261 tagtcaaaaa gcttttttct aagttcaccc tgctgtgtac tatacccttt cacatctata
    43321 taaacgactt caccattcca tttgtatatg gcaaaatcaa ctgtatatgt aatagctctg
    43381 tagcttttcc catctttttt aaatttaggt tgtagttcaa acttttgttg aagtccaaag
    43441 tcttttattt ctccattttc ctttttttct tttaaatata aataatactc tgactcatct
    43501 ttactatcaa atttaattcc atctattaca attttcttat tattgtattt actcaatcaa
    43561 atacttcctt tatcacttaa agttactcat acttattaat atcgtttata aattcataaa
    43621 cctcatgtac actatatcca aacttttcaa acccttttac atatcttctt atggaacttg
    43681 atatacttct acctactata caattctcat atccttgcaa caagttttct ttttcctctt
    43741 gcttcaccat ttgagaaact gctttttgaa acttattcat tgctattcct ccatagtttt
    43801 attgtttaaa attaattttg ctgttctcta ttgcttttat tagcctttct ctcacatctt
    43861 ttacaacagt aaattttttt agactgtttt gggatgtaaa ataatttacc acaccaattg
    43921 caaattattc ttttattcat aaaaccactt ctttctcatt tttcaggata tttgttatta
    43981 caattttcac actcttttag attcaatcta tactcataaa ctctaccagc tataaaactt
    44041 cctattacta atatcacact agctaagata ttcactttta atcatctcct catattcttc
    44101 tctagcctta tttatagcaa taaatatatc ctctccattg tcatataact cttttgccct
    44161 tttaattgta tattctgtcc ttgaaacttc cattattcct ccttaatata ttcaactttc
    44221 cagccacttc ttgttttagt tttcttttta atagcttgat aaactgcttg atattttaac
    44281 cttaaaaaac aggctgcacc atctatagac tcaaattctt ttacttcccc tgtattaaca
    44341 tttctaattc ttactggtaa ctcttttttt atttttctgt ccttctttac tccaaactca
    44401 gctaacattt tttctggctt aggatatatc ttttttccat tttttgtaat tcccaacaaa
    44461 caacaatata atgctaagta gtttctacat gtaaggtcat cttcaataag gttgtccact
    44521 actgagccac taaaatacct ttctacttta aacatttatt tcacctcata tttaattttt
    44581 cagattaaaa tggtaactca tcatcatcta taatttcaaa tccttgaggg tctagtccta
    44641 tggttccatc ttgtacactc tctttatatg aattactcga tttactcttg ctttctaatg
    44701 attgtactga ttttgtactg acttttgtaa atggtctttt ttcacctgat tgagtttgat
    44761 aattgtcaac tcttatattc ccttctaatg ctactagctt cccttttgtt atgtaatttg
    44821 cacagtattc agctgattta cctattactt ctattggtat aaaatcagta tccctttttc
    44881 cttctttatt tatataattt ctgtctacag ctattgtaaa tgatgctact gctgtacctg
    44941 ttcctggtat gtatttgagt tctgggtctc tagttaatct tccaactaat acaacttgat
    45001 tcattttaat acctccatca atattattta aatttagcct tttggctttt ctttataatt
    45061 tcatctaatt ctttgttact gtattgagta aatgtttgtt caaaattagc aaacttattt
    45121 ttgcttacta cactattagg tttttttgac tttttatttt catgttgtaa tttatatgac
    45181 tctaattgtt catatgtagt tatatttgca tccttccact ttttaaggat tccttttaag
    45241 tatgataaat tcatattcat tctttcagca catatctcta tagctctttt aaatactctt
    45301 atatctactt ccttagatac ttctaataac cattcagctg tgactggata tactactcct
    45361 atattttctt catatagctt cttaaattct tttaaatagt tatccacagg cttatctata
    45421 tacatactat taaaactatt aatattaata ctattaatat tagtgtccac attttccgtg
    45481 tccggctgag tcgtgtccgg aaaagtagga catggttcca tgtctatatt tttaggacat
    45541 ggttttttct tctttttatt tacttttaca cctcttctag atatacattc atctatataa
    45601 gttctatcaa atactatttc atatatatta ttttgcattt taccttgttt agatttattt
    45661 ttatgaatct ttatatatcc actaagttcc aattcatttt tgtattttgt aaatgtatct
    45721 tttgatatat tcaattcata acaaattaaa tctctagttg gaaagcaagt tccatttatc
    45781 cctgcaaaac tggataaata agaatatagc attctagcac caacagttaa ccatctatct
    45841 cttgctacta accttggcat aagaccatac ccatcactta atatatttcc tttttctata
    45901 ataactttgt ccatattatt caagagattc acctacttaa ttatcccttt ttctctttgc
    45961 aatttctcat atccaacaca tatctggtca tattcctgtt tacttaaatc tttaacttct
    46021 ttattaaact tataatacac ttcacttttt actctatctg aatctttgcc tattgtattt
    46081 cctattgaaa acaatctttt tatttgattt tgacttattt ttatttgacc attttcactt
    46141 gctttaatag aggagtttgt taagtcattt cttttataat cttcttttcc actagtagca
    46201 tcaaaagtat cattttctgt aatgtttaat aactgaatgt acaaatacct tgtctgatat
    46261 gtttcaattc ctcctaatgc ttgtaactca ttagagcctt ttaattgtaa ttctctcatt
    46321 ggagaagtaa atattatctc ttctgttggt ttctctccat taatcaatgt tagagttgca
    46381 taatcatttg taaaggtcac tatagggcat aatttagctt cttgtaacag ctctgttgct
    46441 tgaggtaaga agtctgctaa ctcaaaatat ttaaagttag caaacttgtt ttgaccactc
    46501 ttttttatat ctaatttgtt aaacttaatt cttacatcca tcaattttat gtaaatatta
    46561 ttcacttcca tcaatttctc cctccatttc tctaatattt ctttctaaaa ctgacacaaa
    46621 accatctata taatcaccat agttcccttg taatttgtat tcttctaaat tttctttgaa
    46681 ttgttgtaaa gtgcatcctt ttctatgttt atctatacaa aattctaatg ctgatatttg
    46741 tccaaatttt acatcccaat ctacttcatc tactgttgta aatcttaata aaaataatct
    46801 atcttctaaa tccttaatta tttcattttt tgttttcatt tattccccct tatgttataa
    46861 tatacctata atttttgtaa cttattttca cttagagcct gtgcgaaggc tcttttttta
    46921 tatctggaca tctattggtc tatctttttc aagttcttct tgatacatca tagcttctct
    46981 aaactcaata gaagcttcta actcaatgtc atgttcaaga ccctctaata tattttgact
    47041 agcaaatttt acggcttccc accacattaa actactatta ttttttgtac cttgtaattt
    47101 acatatttct ttttctgctt gtttaatttg acctattgct attagtctag ctgcttccat
    47161 ttaaatccct cccaatttat tactatctat ataagctaca ttctaaattg ctacaattat
    47221 tacacattaa accttttaga atgttatatt acctattcta aatccattct ttttgtgcta
    47281 taatttgttt gtgttatatt ttatttttta tttttttgtg tgttggttat ttgaccagca
    47341 cttttttatt taatactcca accgatattt tcttaccagt tttaatatct ttaaatacta
    47401 tgtctgctat agcttttcca tcttttttaa gagttactat attattctta ttagtattaa
    47461 ggcttaacaa tttcatcccc cctctctact gcttttaaaa gttcatccaa atttttacct
    47521 tgatttcttt caataaaatc atcaacttca tatcttgaaa tttttctacc atcacctcta
    47581 gccagtgatt ttatcaaacc tgtgctcact aacctacgca taaaaactgt atctaatttt
    47641 aaaattcccc ttgcttcttc tactgttatc aaataatttg gatagcctct ttttattaaa
    47701 acaactatat ctttaggttc tagtactttt accttttgtt caacagtttg actcttaact
    47761 ctatctgttt cttgtttatt tatctctatt tctattaaac tttttaaact atcactaaat
    47821 tgttttgcta tactttcaga aatatccatg tgaaaattct ctcccctctt tttaatgaaa
    47881 tatataaaat taattttata tctacgcatt attaccaaaa tcctatatat attattttca
    47941 gctccatacc tcatgttata attgactcaa gaagtattcc cacatacttc caatacataa
    48001 gcaaaggatg gtgaaaatat gaataaggac gagttaaata aaatttgcga cgaagtgtac
    48061 aacagaatac taaaaagtga atattcagat gaaacacttc ttaggaataa aattagtgaa
    48121 tttatttccc taaaagaaaa atctgctagt gaaataagta atacagagct ttgtatgttt
    48181 ttttattttg agaatattgc atttactaaa aactatgtta aagaagtctt atctgaaatt
    48241 cttttagata aataatcact tatttcatct atttttattg gctttgtttt ttcttcaagc
    48301 aaagccaatc ttttccctat atcagactct ttcagaagat aatcagtaat atagtgtaaa
    48361 tctttttctg taatctttgt atccttttca ttcattaaaa tacacctctc ttttatatat
    48421 tttgaatcta attttctaag tactaaccca acattgaagt ttgataacta tattcttttt
    48481 ctatatctgg taatatgtta tttactttca ataagtcata taaaaacaat cttccctttt
    48541 gagtccactt agttgtcatt tttacatcag tcattccatc acttctagtt atgtctattg
    48601 tttctgaatg agtgtatccc ttgccttgat actgtttata taaaagccat tgtccacttt
    48661 gtttgtattg aatccctctt tcatgaagta ttttattcat ttcttttcca ctcattccat
    48721 agtcttttgc tatttgagtt atagtgacaa gacctttatt ttttaatatc atatctgtat
    48781 aatctgcctt tggctttagt tcttttatta cttggtcttt catttttcct tctagttgta
    48841 atttctcatt tgcttctact tgctctataa gatgttgtaa cgcttcttta tatgtagttg
    48901 gtaatttagt ttgttcattt tttaaagctt tttccatttc atcaaatttt tttgtatata
    48961 ttcctgtaaa tgctgtacct tttatgcctg tcattttatt tgctataaag tcacaaccta
    49021 tctttgttat tgcgtaacat ggtttctttc ttttataatc atctaaataa gtattttcaa
    49081 taaaataatc agatgggtac aaatctgtac tcatgttttt atttgcttct tccatttggt
    49141 ttatataatt tcttatatcc cttagtaaaa tcttgtgttc tttttcaacc attagagcta
    49201 tatccctact atcagttgtt agtttattat taacttttac tatttttaaa tctttcatat
    49261 ttacaaactc ctctctataa aatgtccatc attttattgc cattttagca actaattctg
    49321 aaaaaaaata gtctgttgat actccatata attcagacaa cttttttatt tcatttgctt
    49381 tgaactcatt tttaccatta agtttaagcc taaaaccaaa taaactaaga cctaacaaat
    49441 ctgcaacaaa tttttgcgta tgttggtttt ctttcattaa ccctttcaat ctattgacat
    49501 acattttttc accctctaac tttttgctat ataagcaagt tcttgtttat ataatagcaa
    49561 caaaaatgtt aaaagtcaat atttttgttg cttttttggc aattttaatt gtaataacaa
    49621 aaaaagttgc tataatgtaa ataaaagttg ctataatgaa aactacaata aaggatgatt
    49681 ttatatgttt agattaaaag aactgagaga agaaaaagga atatctttag atagattaag
    49741 tatggattta aatgtaaata aatccactct atctaggatt gaaaatgggt taagagagcc
    49801 aaaacaatct tttattgaag actgttcaaa ttattttgat gtatcaacgg attacttact
    49861 tggtaaaaca gacataaaaa atcccaatca agtagtaaaa tctttaaatc ctattttctc
    49921 aaaaagatta agagaattaa taaaggaaaa aggtgtcaca ttaaatatat taggcaaaaa
    49981 attagaaata agtactgatt tattatctaa atatgaaaat aatataattt cacctaaaag
    50041 tgatgatatt ataaagcttg ctcattattt tgatgttaca acagattatc ttttaggtac
    50101 aacacttgtt agaaatcata tagacactgt agccgcacac aaagcaaatc cacatgagga
    50161 tttacctgaa gaagctcaag aacagcttaa tgattatatt gaatttttaa tgaataaata
    50221 taagaaatag gaggaactat gtcagaagaa caatatttag aaaatagagt gaataaacaa
    50281 ataaattggt acagctctaa aagtagtaat attaaaaaaa aattttttac tattcaagtt
    50341 atagaaataa ttatagctgg aataataact gctttatcga aattttcatt tgaaaatgaa
    50401 catatagcag atataataac tattttaagt atagtagtaa ttattttttc atctttaaga
    50461 aatttattta aatggcaaga cttgtggatt aaatatagat atactgcaga attattaaaa
    50521 agagaaaagt ttttatttct aactaggact aatatttaca taaatgaaaa tgccttcaat
    50581 ttatttacag aaagaattga agacattcta aatagagaaa atgaaaattg gacagaactc
    50641 aatttgaact cttaatgata taaaaaacta tacaataaat gatgttaaag gaacttcatc
    50701 atctacagct tcatatgttt tttcaaaaat atctggctta caaggatatt gttctcctct
    50761 tacaccagtt ataatccagt caccaataga tgcttttaaa ggaccttcta atgtttgtat
    50821 aataaattct atatctgtct gataagcttc tataacaact ggcttttttc taaacttttt
    50881 tacttgcata aaagcacctc cataaatatt attttgtaaa atattatatc acaaaagaaa
    50941 ggataatata ctatgcctaa attatatgat tatagagttt tcataagtca tgcttggaaa
    51001 tatggtaatg actatgatag attgataaac ttactaaata atgctaataa ctttagttat
    51061 tataattatt ctgcaccaaa agaaaaacct ttatttccag atggaacacc ttttagcaat
    51121 tcagatatag ctaaaaaaat aacagataaa atatctcctt cacaaataac aattgttata
    51181 tctggaatgt atgttcaata taaagattgg ataaaatacg aaattgatga atcaattaga
    51241 attggaaagc caatattagg aataagacct tatggaaatt ctaatattcc tacttatgtt
    51301 tctgataatg ctaataaaat tgttaactgg aatacagatt ctatagtatc tgcaattaga
    51361 gaacttgtaa aataaatatt cttatggaga gtgcatttat taaattaact agtaaccctt
    51421 tactgggtta ctttaaagct ctacatcaaa catactttct ttttttaggg ggatttcaat
    51481 gaataaacta gacgcacttt tagacttagc aaataatgaa gagatagaaa tttactacac
    51541 tgacaaaata gcagatgaca taaaaggatt gtatataaac agacaaggac taaagattat
    51601 atcattactt aattcattaa aacaaaacaa tgctaaacta atagaaatct tagcagaaga
    51661 attaggacat cattttacca gtgttgggaa ctatgtatct tcaaaaaaca gttacaaaaa
    51721 taaaatcttg atagacaaaa ctgaaaacaa agcattaaaa tgggcatgtg aatttcttat
    51781 aacagaagaa gaaataatac atgttattaa ttcacacgct acaagtgtat acgaaatagc
    51841 tgaagaatta caagttagca tcaacttctt actaaaaaga ttagaatttc tatcaaaaaa
    51901 gaaaagcatg ttggacttag gaaataatag atttttagta ttaactaatt tgccaaattt
    51961 ctacatatat gaggatattt tttaaactca tttattctac ttttatagat tttttactta
    52021 ataaatatat atttcaatat tattataata aactacacat aaaagctaaa aaattgtaag
    52081 aatattaaga aaataattaa gtaaaaacca gataaacaaa attaagataa tattgtacat
    52141 aacaaaagta tataaagagc agttaatctg ctcttttata taaacaccaa acaaacatac
    52201 attctaaaag ggagggatac tattatgaaa ggtggagtaa gaaaaagaag taacaaatgg
    52261 tattactact ttgacctagg catagtagaa ggaaaaagaa aaaaagtaga aagagttgga
    52321 ggcaatacta aaaaagaagc cgaaaaagcc ttaagagaag cactaaatga atatgaaaac
    52381 tctggcatag tatttgaaga aagcaatatc agtttatcag actacttaga cttttggtac
    52441 aaagaatatg tcttacttaa ctgtaaatac aacactcaag aaagctaccg aataaacata
    52501 gaaaaacata taaagccaaa actaggagct tacaaagtaa aagctttaac tcctgcaata
    52561 ctacaaaact tcataaataa aaagtacaaa gaggattact ctcaaaatac attacaagta
    52621 ttaaaagcca tattacatag gtcattaaaa tcagcagtcc atccttacaa acacatacga
    52681 gaaaacccta tgcaatatgt aagcatacca aaaactaaat ctaaaacaga aactaataaa
    52741 gttaaaacta ttacattaga agaatttaat caaatactaa atatatttcc tcaagattca
    52801 tttcaacgta tagttttact aattggattt catactggta tgcgaagagg tgaaattatt
    52861 gcactaaaat gggataatat agaccttgat aataaaacta tcacagtaaa gcatactttg
    52921 attaaaaaac caaatggaat gtttgaatta gggcaaccaa aaacagaaag ctcttgcaga
    52981 actatattta caggtgacac tttaataaag gcattaaaag aacataaatt atatcaaaag
    53041 aaaatgaaat taaaatatgg agaattttac tttgatagtg attgggtatg taccaaagaa
    53101 aatggtcaac aagtgaatac tcacacttta gacactatag taagacaaat tcgagtagct
    53161 ttaaacaatg acttccattt tcattcttta agacatgcac atgctactct attattagaa
    53221 aatggtgcta acattaaaga catacaaaac cgtttaggtc atagccaact atcaactaca
    53281 atggatacct attcacatgt aactgataaa atgaaaaatg aaactgtaga tatatttgaa
    53341 aaaattacaa attagagttt gccacccaaa aatataatac ggtggcaaat gggtggcaaa
    53401 atctaattta tctattttaa aagctaaaat tatcaaattt atatagtcag ctatacgctt
    53461 gtaattt
//
LOCUS       DAGSWI010000008        53255 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.008, whole genome shotgun sequence.
ACCESSION   DAGSWI010000008 DAGSWI010000000
VERSION     DAGSWI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.008"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..230)
                     /locus_tag="MJF80_000424"
     CDS             complement(<1..230)
                     /locus_tag="MJF80_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBZ0279105.1"
                     /translation="MRQYIHDKLREITEEEKNILEGNYIIDKSIYTDNSQFIIDSNKL
                     LNIDELIHIRKHTRFAQFPKHKHNYIEFNYVY"
     gene            complement(354..635)
                     /locus_tag="MJF80_000425"
     CDS             complement(354..635)
                     /locus_tag="MJF80_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279106.1"
                     /translation="MKDETLEDILKQKTQAERYILEYIVTKNVKKLDNKKIVSILHTI
                     LCWEEINGTIVLTDKNILSLDEDIMISKHRRFIDIPKHKHNFIEFHLMS"
     gene            821..1141
                     /gene="rhaM"
                     /locus_tag="MJF80_000426"
                     /pseudo
     CDS             821..1141
                     /gene="rhaM"
                     /locus_tag="MJF80_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
     gene            complement(1810..1950)
                     /locus_tag="MJF80_000427"
     CDS             complement(1810..1950)
                     /locus_tag="MJF80_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279107.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(2210..3370)
                     /locus_tag="MJF80_000428"
     CDS             complement(2210..3370)
                     /locus_tag="MJF80_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HBZ0279108.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            complement(3541..3744)
                     /locus_tag="MJF80_000429"
     CDS             complement(3541..3744)
                     /locus_tag="MJF80_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HBZ0279109.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            complement(3831..4865)
                     /locus_tag="MJF80_000430"
     CDS             complement(3831..4865)
                     /locus_tag="MJF80_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HBZ0279110.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEALKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            complement(5387..6748)
                     /gene="rlmD"
                     /locus_tag="MJF80_000431"
     CDS             complement(5387..6748)
                     /gene="rlmD"
                     /locus_tag="MJF80_000431"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBZ0279111.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVKA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            complement(6775..7497)
                     /locus_tag="MJF80_000432"
     CDS             complement(6775..7497)
                     /locus_tag="MJF80_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBZ0279112.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNTGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(7497..7826)
                     /locus_tag="MJF80_000433"
     CDS             complement(7497..7826)
                     /locus_tag="MJF80_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBZ0279113.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            8156..9082
                     /gene="fba"
                     /locus_tag="MJF80_000434"
     CDS             8156..9082
                     /gene="fba"
                     /locus_tag="MJF80_000434"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBZ0279114.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(9217..10848)
                     /locus_tag="MJF80_000435"
     CDS             complement(9217..10848)
                     /locus_tag="MJF80_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0279115.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(11331..12368)
                     /locus_tag="MJF80_000436"
     CDS             complement(11331..12368)
                     /locus_tag="MJF80_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBZ0279116.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(12387..13172)
                     /locus_tag="MJF80_000437"
     CDS             complement(12387..13172)
                     /locus_tag="MJF80_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBZ0279117.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(13205..14338)
                     /locus_tag="MJF80_000438"
     CDS             complement(13205..14338)
                     /locus_tag="MJF80_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBZ0279118.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(14386..15513)
                     /gene="hadC"
                     /locus_tag="MJF80_000439"
     CDS             complement(14386..15513)
                     /gene="hadC"
                     /locus_tag="MJF80_000439"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBZ0279119.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(15513..16739)
                     /gene="hadB"
                     /locus_tag="MJF80_000440"
     CDS             complement(15513..16739)
                     /gene="hadB"
                     /locus_tag="MJF80_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBZ0279120.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(16754..17554)
                     /gene="hadI"
                     /locus_tag="MJF80_000441"
     CDS             complement(16754..17554)
                     /gene="hadI"
                     /locus_tag="MJF80_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBZ0279121.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(17593..18792)
                     /gene="hadA"
                     /locus_tag="MJF80_000442"
     CDS             complement(17593..18792)
                     /gene="hadA"
                     /locus_tag="MJF80_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBZ0279122.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            19469..20467
                     /locus_tag="MJF80_000443"
     CDS             19469..20467
                     /locus_tag="MJF80_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBZ0279123.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            20829..21356
                     /locus_tag="MJF80_000444"
     CDS             20829..21356
                     /locus_tag="MJF80_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBZ0279124.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            21383..21979
                     /locus_tag="MJF80_000445"
     CDS             21383..21979
                     /locus_tag="MJF80_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBZ0279125.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFAKDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(22058..23641)
                     /gene="asnB"
                     /locus_tag="MJF80_000446"
     CDS             complement(22058..23641)
                     /gene="asnB"
                     /locus_tag="MJF80_000446"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBZ0279126.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
     gene            complement(24243..25079)
                     /locus_tag="MJF80_000447"
     CDS             complement(24243..25079)
                     /locus_tag="MJF80_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBZ0279127.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(25103..26569)
                     /locus_tag="MJF80_000448"
     CDS             complement(25103..26569)
                     /locus_tag="MJF80_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBZ0279128.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(26572..28434)
                     /locus_tag="MJF80_000449"
     CDS             complement(26572..28434)
                     /locus_tag="MJF80_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBZ0279129.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGKRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPIMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            complement(28681..29544)
                     /locus_tag="MJF80_000450"
     CDS             complement(28681..29544)
                     /locus_tag="MJF80_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBZ0279130.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(29599..30531)
                     /locus_tag="MJF80_000451"
     CDS             complement(29599..30531)
                     /locus_tag="MJF80_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBZ0279131.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            complement(30617..31168)
                     /locus_tag="MJF80_000452"
     CDS             complement(30617..31168)
                     /locus_tag="MJF80_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0279132.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            complement(31397..31888)
                     /locus_tag="MJF80_000453"
     CDS             complement(31397..31888)
                     /locus_tag="MJF80_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279133.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(31916..32476)
                     /locus_tag="MJF80_000454"
     CDS             complement(31916..32476)
                     /locus_tag="MJF80_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBZ0279134.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            complement(32628..33785)
                     /locus_tag="MJF80_000455"
     CDS             complement(32628..33785)
                     /locus_tag="MJF80_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBZ0279135.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(33822..34763)
                     /locus_tag="MJF80_000456"
     CDS             complement(33822..34763)
                     /locus_tag="MJF80_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279136.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            complement(34850..35542)
                     /locus_tag="MJF80_000457"
     CDS             complement(34850..35542)
                     /locus_tag="MJF80_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBZ0279137.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(35651..36463)
                     /locus_tag="MJF80_000458"
     CDS             complement(35651..36463)
                     /locus_tag="MJF80_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBZ0279138.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(36514..37425)
                     /locus_tag="MJF80_000459"
     CDS             complement(36514..37425)
                     /locus_tag="MJF80_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBZ0279139.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(37484..38215)
                     /locus_tag="MJF80_000460"
     CDS             complement(37484..38215)
                     /locus_tag="MJF80_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBZ0279140.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            38304..38555
                     /locus_tag="MJF80_000461"
     CDS             38304..38555
                     /locus_tag="MJF80_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279141.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            38788..39207
                     /locus_tag="MJF80_000462"
     CDS             38788..39207
                     /locus_tag="MJF80_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279142.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVPDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            39232..44289
                     /locus_tag="MJF80_000463"
     CDS             39232..44289
                     /locus_tag="MJF80_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBZ0279143.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIKGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(44474..45211)
                     /locus_tag="MJF80_000464"
     CDS             complement(44474..45211)
                     /locus_tag="MJF80_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0279144.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(45230..46165)
                     /locus_tag="MJF80_000465"
     CDS             complement(45230..46165)
                     /locus_tag="MJF80_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBZ0279145.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            46373..48949
                     /locus_tag="MJF80_000466"
                     /pseudo
     CDS             46373..48949
                     /locus_tag="MJF80_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            48961..49632
                     /locus_tag="MJF80_000467"
     CDS             48961..49632
                     /locus_tag="MJF80_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279146.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            49659..50000
                     /locus_tag="MJF80_000468"
     CDS             49659..50000
                     /locus_tag="MJF80_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279147.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(50360..53002)
                     /gene="adhE"
                     /locus_tag="MJF80_000469"
                     /gene_synonym="adhC"
     CDS             complement(50360..53002)
                     /gene="adhE"
                     /locus_tag="MJF80_000469"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBZ0279148.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT        17759 a         8929 c         6350 g        20217 t
ORIGIN      
        1 ctataaacat aattaaattc tatataatta tgcttatgtt ttggaaattg tgcaaatctt
       61 gtatgttttc ttatatgtat caactcatct atattcaata atttgttgct atctattata
      121 aattgagaat tatcagtata aatactttta tctattatat aatttccttc taatatattt
      181 ttttcttctt ctgttatttc cctcaactta tcatgaatat attgtctcat gagctacctc
      241 cttagttagt tcaactacta gttgtttcaa tttaaaatta tttttagcat taaatatata
      301 ataattatca catctttttc ctttgtgtat agttcctgat aaaaaattat atattaactc
      361 attagatgaa actctataaa gttatgttta tgtttgggta tatctataaa tctacgatgt
      421 ttagatatca taatgtcttc atctaaagaa agtatatttt tatctgttag tactatagtt
      481 ccatttattt cttcccaaca taaaatagta tgcaatatag acactatttt tttattatct
      541 agttttttta cattcttagt aactatgtat tctaagatgt acctttctgc ttgtgttttt
      601 tgtttaagta tatcctctag cgtttcatct ttcatttata tccccccaga tttagctata
      661 ctttattaca gttttaaatt atattacaaa tattctttat ctaattgtat catattagtt
      721 tttgacagta ataatgttaa attatgacat ttaaaaatac ctttttttgt tttaaaatac
      781 atttataggt aaatacttaa aatttaggag gtattttttt atgaatacag tacgcaaatg
      841 tttcaagatg aagcttttta aaggatttga aaatgaatac aagaaacgtc atgatgaatt
      901 atgatctgag atgatagata tgattcatga atatggtggt agtaactatt ctatttttct
      961 agatgaagaa actaattatt tatatggata tatagaactt aaagatgcca aactatggga
     1021 taaaagttca gagacagcaa tctgcaaaaa atggtggaat tatatgtctg atataatgga
     1081 aactaatgag gataatagcc ctgtatctat agaacttaaa tctatgtttt atttaaaata
     1141 aggacaaaaa ttattataaa tcacaaaatg gggatatatc aaaataaaaa tttgaattat
     1201 ctcctttttg tataaaaaag ataagtgatt cctaaaaacc tctagtctat gtgataattg
     1261 ttctgtgaaa aactaaaaac atatttcgat tatagtagct aaagatgtta taaacaagtg
     1321 gtatttctat tagacattga aacaattatt cccaaagatg attcagtaag actacttaac
     1381 ttaactttag aataattgat atatcttgtg ttgtttatat taaattttta atttaagtaa
     1441 tagtatatgt aatctttagt catcttctta ccagtttttt atccactttt ctaatgaagt
     1501 tattttttca tatgataatt cctcttgaaa taatgatact aaaagacttt ttataaaatt
     1561 ataacttaaa agtttatgta tttttttgat atgaatttat agtacttatc ttctttaatt
     1621 gagattgtgt atatttaact aaacattaca acaataattc tatgaaatca aatcaattat
     1681 ttacatattt ttttagaaca ataataagta tatattcaca aaattttgtg ttaatatttt
     1741 tagtttgtga tataataaaa gcaaggaaac atattttact taactttgag tgatgtttcc
     1801 ttcaacaaat tattttgaag atttactttt ctggaacttt atattaagtt cccagccact
     1861 cttagttggt cgctcagagt ggctctttaa tttatcgtaa atgtaactgg ctataacacc
     1921 agttataata ctcatcatta gattttccat agttttcacc tcctctcata agaaagtgaa
     1981 gaaacttcca tggaaacacc actcatagct ttttcaaata tttattcctt gccatattaa
     2041 ttatatcata aatattggaa acataaaatt ttaatactta tcgtctttat tattattttt
     2101 tataattaat aattttcttt ttaataatac ataatataaa tttatatttc ttcaataaaa
     2161 aagacaatct taattaattg tcttttttaa tatatatttg gctaatacat tattttacac
     2221 aaacattaca agggtctatt ttaccttgtg acttagcttg ttttaatgtt actttattta
     2281 ttgttttact tctttttagt gtagaacaat tttttgttag atgatatgat tttccattag
     2341 gagttacata tactgtactt ttatcagtta atccaggatt attagagcta ggctcattac
     2401 ttggtttgtt cgtattattt gaattagtat cttttacaat acctgtattt gaatccccaa
     2461 cattattagt tatatttaaa cattgtttta taacaacatc atcaatattt ccaatagcag
     2521 ttattttact tgcatttttt aatatactct tattacttcc atttccaact aacactatag
     2581 gtgttttttg tgcaattgta gaaccaacta aagaataaac taaatcagtt ccacttgcta
     2641 tgtaaaattc ttttgagcca ttataaaatt tatttattat ttgtttattt gtatcgtatc
     2701 tatctactcc acctattctt gttgctttag tttcatttat taacttatca ctcatactga
     2761 cttttccacc aattgcataa gtttctaatc catctgtagc aaaacttgta gtatttccat
     2821 cagttaatac tattggagat ttatctctaa ctgctataga agcaacactc atagcatctg
     2881 gttcgccttt aaaagcatta gttaatatta cttttttaac cgtacttact cttcctattt
     2941 cttttgcaac tttatgactt gtatctattc tatcagttcc acttattcta tttatgctta
     3001 tttttttatc acttaatatt ttctgaactg atgctcctat cgaatttttt cctccaatta
     3061 gatatacttc ttttaccttt tctaatcttt ttaatgtttc tgaagggatt gtatctttct
     3121 tagttaacaa tattggggca tttttagctc ctgacaatcc acttgctgac aatccatcag
     3181 ataatccatt ttcagaatta ataagtattg caatatcata attttgttta tctgctatta
     3241 aacctgctgt ttcatactta tttgtacctt ttattgaatt aatagaacta agagcgtttg
     3301 atgttggaca actagcaatc aataatgaaa cagacatccc tattgccaat agtattttgc
     3361 ttaatttcat aatgaaatcc tcctataaat tttataatat tacttaaata ttataattct
     3421 acataaaaga atattttcct ttatgaaatt agtttatatt tcaaaatttt gtcacttttt
     3481 aaatctatat actaaaaaat agactatttg taacagtcta tttcttagta tatcaaatat
     3541 ttatttaggt ttacgtttct ttgtactagt ttttgtatat gatattccag ttccaggtat
     3601 actcaatgta gctgattttc ttccttttga attaactgac actctagcac ctttaacacc
     3661 agcacttata cctacactct tcttacctaa atttaatttg agtcctcctc ctagatttat
     3721 gcttttccta aagttaaatc ccatagatat aaccttccct tctatatttt aaatatttat
     3781 acttaaaatt atatttgtta tacacttgtc atcattggta tatctagcat ctatatcttt
     3841 ttacgttttt tcttcttata tactactcca gcaactacag ctgctgttcc agctccaacc
     3901 ccataagtag catttctact tttctcttct tctctttttt ctaaaccttc atcatataca
     3961 ccttttaact tagtttcaat agacttcatt tcatctggta tactaaaatc ttcttcattg
     4021 taaccttgat tatatacact ttcttttgct ttaggtaact taaccttacc attatcatat
     4081 ccttctttaa accaattagc atatttaaca ttacctatag ttgatgtgtc aaatctttct
     4141 cctttgatag cactctcaaa tccttgagta gtgtaatcat tttttaattg ttcttgtatt
     4201 tcgtcataag cattattata agcatctata aattcagaat ctatgttttc ttcaaatgtc
     4261 attttttctt tatctttatt gctatcctct atccctttag ctttatattc ttctatcttt
     4321 ttagtcttat attcattgta tccagttgaa tatccggtat cataagcatt ttttaaagct
     4381 tctttttcat atgtattatt agatttagca cctgtaagcc ctaaattata tccagtctct
     4441 ttagccacct tttcttcctc ttctaattta gccttacctt tttcatatcc ttctgagtac
     4501 tttgattcat atgtatcact ataatctcca ctataatttg atgaactata atttccttta
     4561 tacccatctt catacccttg attatatcct ttttctccaa cagacctctt ttgcttttct
     4621 tcattattaa ccgaagttgt tgttccagaa ctgcttcctc ctccagtata aggacaacca
     4681 ccattattat gtaaatgagc tggataacca ccacaatgat agtgataact tcctaatcca
     4741 gatttatttt tattatcacg atgacctcct gatgaatcag ttcttcctga atgagcatac
     4801 gaaaagctcc ctacactaat catagttata ctcattgcaa cagctattaa ctttttctta
     4861 aacataatct atttccccct aaaatattct atgattttat tatatcagtc aatatattat
     4921 gtttaaaccg ttaaaattcg acaatgtaaa tatttgatga attgtatttg taaataaatc
     4981 ttttagcata tcttgaatat ctgtagttgt tttgacatca tattcttcaa taagtttttt
     5041 aatatttcgt ttttgttaaa cataaaaagc gtgcactttc ctttgtatag tgatatttga
     5101 tattttaatt atcactactt tagaagaata cacactttat tttaaaaacc ctgatactat
     5161 gttattagaa aatggtacta atataaaaga tatccagcat atactcggac atgctaaaac
     5221 ttcaacaact atggatacat atttacatgt aactaataac atgagaaacc agacggtaaa
     5281 tatttttgaa atcattgtag aataaatttg ccacccaaaa ataattatgg tggcaaatag
     5341 gtggcaaatc tacttttaag acacactata aaagtctgaa accctgttat tctctaataa
     5401 gtttaactac agtctcacaa tgtggcgtat ttgggaacat atccatacac ttaaccatct
     5461 taatctcata accatacccc ttaaaatcaa ccaaatcaac aaccaaagac ttaggattac
     5521 aagaaatata aacaatctcc ttagcaccaa atccacaaat atccctaata gcatccttat
     5581 gaatgcctgg tctaggaggg tcaacaataa tcaaatcagg cttgtccttt aaatccttaa
     5641 caaccttagc cacatcccca gcaataaact cacaattagt aagtccattc aacttagcat
     5701 tctcattagc agcaacaaca gcttcctcaa ctatctcaat accatacact ttcttagcag
     5761 caccagccat aacctgtcca atagacccag taccactata caagtcaaat acaactttat
     5821 cattgtaatc tccaataaaa tccctagcaa tactataaag aacctcagct cccttagtat
     5881 tggtttggaa aaacgaaaat ggtgaaatct taaacttaag acctaaaatt tcttcttgta
     5941 tataatctct tccatacaat actcttaact catcacattg gactgcatct gctaaaccat
     6001 cattaatagt atgaagtaca cctactaact cagcttttaa atcaatccct aaaagcatat
     6061 ctttaaactc atacatatca aaatcttctt gagaagaagt tactatatta accataatct
     6121 cattagtatg aatccctttt ctaactacaa gatgtctcaa atatcctttg tgattcattg
     6181 ttctataata aggaagcttt ttttcattga aaaactctac actagaaact aatatctttg
     6241 agaagtcttc atcaaccaac atacactcct caacagtttg tatatctata tgctttcctt
     6301 ttttatgaag acctaatgtc aaaggtccat tttttacttc atctccaaaa gtatattcca
     6361 tcttatttct ataatatttg ttttctggac ttccttctat ccctaaaaac tgaaatccaa
     6421 ataaatcttg ctttaaaaat aaatccataa cttgtttttc ttttatttct aattgttttt
     6481 catatggcac agacaatagt gtacatcctc cacactctct aaagtgcttg caagtttctt
     6541 cagtttcaag tggtgagttc tctaataatt ctatcatctt aacttcagct ttcttacttc
     6601 taactttttt aactccagct ttcacctttt gaccacttat tccacccttc atatggataa
     6661 ctctatctcc aacttggctt aaagatgtgc caccaaattc cattttatct acttcaaatt
     6721 ctataacatc ttttttcttc acagacattc ctccaattta ctatagtatc tttattatct
     6781 ttcttcaatc aattttactt cttcatctgt taactctcta tactcaccta actctaaact
     6841 ttcatccaat tttaaattgc ccatagacaa tctttttaag tatactactt ttttgcctac
     6901 gctttcaaac attcttttta cttggtgaaa cttaccttca tgtattgtaa gctctatttc
     6961 tgattcatca tcacttttta atatatttaa ctgtgatggc atagttttat atccatcatc
     7021 aagcactaca ccttctaaaa aagcttctac atcttcttca gtaactactc catcaatttt
     7081 tgcataataa gtcttaggca catgcttctt aggagataaa actctatgag aaagctttcc
     7141 atcattagtt aaaactaata atccctctgt gtctttatct aatcttccaa caggaaatgg
     7201 ttcaaaagct aagtatgata aatcaattaa atctaatact gttgggtcat atttatctgt
     7261 agttgctgat atatatccat caggtttatt catcatcaaa tatatatatt ctctatatat
     7321 aacttcttct ccattaaata atatctcatc attttcagtg tctacttgag ttcctgtatt
     7381 tgaaacttca cttccattta ctactacaga cccttgttta caatacttct ttatttcact
     7441 tctacttcca taacctaaat ttgaaagaat tttatctata cgttgctttt tagccattaa
     7501 aaatgctctc cattctcata atatttttct ttttcaaata ctgtaaatgc taaatccata
     7561 ctttctacat ctaaattatt tcttatacta tcagttatta ttcttgctac tatatcttgt
     7621 acttcttgtc ctctatcaaa ccaagctact tcaacaaaag gatatacatc tgctatttta
     7681 ccatctctta ttgcaactga ctttatacat tctatttcaa agtagtctct tggacatttt
     7741 acagcttcaa ctaaatcatt tatcattttt tcacttattt tacatatatc attttcatta
     7801 attcctctta tttttatctg tggcatagtt tatctccttt attttaaatt tttagtgaat
     7861 tatcatccac ttaattccct tcgataacgt ttgaagtggt aacttcttgg tcaagacaac
     7921 tattgttatc agtatctcca aactaataag gtagttccta cccttatagt tagcattata
     7981 tagctaattt tcctaaattg tttatattct ttattatttt atttaatcct attgtcttct
     8041 atttaacatt atacttgtaa tttatagtta ttacaattta ctatatactt tttttgattt
     8101 aacgttataa tataagtgat gggtaaattt taaaagttaa taggaggagt ttaaaatggc
     8161 attaattact acaaaagaaa tgtttaaaaa agcttacgaa ggtggatttg ctataggtgc
     8221 atttaacata agtgacttag aacaattaca aggtgtttta aaagctgcta aagctaaaaa
     8281 ttcttatgtt atgatacaag cttctatgtc agctgttaaa tatgctggac ctcatacatt
     8341 agttgaaatg gttaaggctg cttctgatga aataggtgta gatgtagctc ttcacttaga
     8401 ccatggtcca aatatggatg ctattaaaac ttgtattgat gctggtttct cttcagttat
     8461 gatagatggt tctcattttg actttgaaga aaatgttaga ataactaaag aagctgttga
     8521 atatgcacac tctaaaggtg ttgttgttga agcagaactt ggtgttctag ctggaacaga
     8581 agatgatgtt acttctgatg ttcacaagta tactcaacca gctgaggcag ttgagttcgt
     8641 tgaaagaaca ggtgttgatt cattagcaat agctattgga acttctcatg gtgctttcaa
     8701 attcaaagga gaagctaaat taagattcga tatactagaa gaaatccaaa gcaaattacc
     8761 aggtttccca atagttcttc atggtgcatc tgctgttgac caaaacgctg ttgctacatg
     8821 taatgaattt ggtggaaata tagctggtgc taaaggtgta cctgtagata tgttaagaaa
     8881 agcatcttct atggctgttt gtaaaataaa catggatact gacttaagac tagctatgac
     8941 tgctgctatc agaaaattct tagctgaaaa tccaaaagaa tttgacccaa gaaaatacat
     9001 tggtgctggt agagatgcta tacaagctgt agttgaaagc aagatagatg atgttttagg
     9061 ttctgctaac tctataaact aatataactt ttaaatattt ataaaagaaa agagatgact
     9121 taaagtaatt aaatttattt ttaagccatc tcttttaagt tgtatcaatt atgtatctta
     9181 taatatttta attatattgt ctttttaaac tgacagctaa acgtgaatat gtccactctt
     9241 tatctttcta tggatggtag aaggatatat acctataact tcagcagcct ttgttattga
     9301 accatactta tttatagcct tacttatcat tatttggtcc actattttat atgcatcatt
     9361 aagattcata attccattta ccactattgg tgataaatca tctgtttcat tatcaatcat
     9421 atctaaattt atcagcatgt taaattcatc ttctccaatt acatttttag ctgataatac
     9481 tacaaatctt tcaatgatgt tttttaattc tcttatgttt cctggccaag aatattcttc
     9541 taataactca ataactttag gcgaaacttc tacttctcta ttgtactttt cattgtaaag
     9601 ttttaagaaa taatttacta atggtactat atcctctttt ctttctctaa gaggaggtat
     9661 ttttactgga ataacactta atctatagta caaatcttgt ctaaatttaa ggttatttct
     9721 aaggtcttca tgagaaatat tagtagcact tatatatcta acatctattt ttataggctt
     9781 actaccacct attctagtaa tagtattttc ttgtataacc ctaagtaatt ttttttgcat
     9841 ctgcattggt aactcaccta tctcatccaa gaaaatagtt ccaccattcg cctcttcaaa
     9901 aagtcccttt ttaccttttt tcttagctcc agtaaaagaa ccttcttcat atccaaaaaa
     9961 ctctgactct aataattcat ttggtatagc tccacagttt atagctataa aaggtttgtc
    10021 tctccttgaa ctatacttat atattgttct tgctattatt tcttttccta cacctgattc
    10081 tccatttata aatatagaag aatctgaggc tgcagctctc ttaacaactt tttttatatt
    10141 gtccataatc ttactttgac ttatatactc tgtgtcttct gatatatcac tatattttat
    10201 ctgagattca agcttaataa cccttgaaat atctctccct gttagtactc ctcctgtaag
    10261 ctttccctta gaatcccatc ttggaactgc cgtatatgca ataagcttcc catctgggta
    10321 aacgatattt ctataaatct ttttcttcac tttaagcatg tttaatatta ttggttcttc
    10381 aactaatcct tgtctcacaa ggtctgttat atttctacca agtacctcag ttcttttaaa
    10441 aggtaatatc tcatcactga cagtgttcat cttttcaatt accccatctt tgttaaacac
    10501 aacaacaact tcatctatat gctctattat atcttcaatt actttatcag atattagaga
    10561 cttctcaaat aaataaacta tattattttc attattatta tctttcatat tgtatatatg
    10621 tgcagtcatc cttaactctc tgaaagctat tttcttttta ctatctgtca gtatttctgt
    10681 tttgtcaaaa tttgaatcta attcatttat atttctaggc ttttttatat ctagaaaact
    10741 aaaaatacta tctattagat gattacagta tattatttcg cctgattctt tacataaaat
    10801 ggctggattt tctacatgtt ctataagccc tattaaatta ttatccatta atattcgcac
    10861 ctttcattaa gtaatttatt gaactacata caaaatttat tgtttgctta caataaatac
    10921 catcagcaca tataatcaac tccttataat attcgtcttt tcaagtagtt tatttcatta
    10981 aaacgtaact aagatataat tttaatgatt atagtttgtt aaaaatttca ttttgctttt
    11041 aattgtattt tatcacatat ttagtaatta tttgtctatt taatacaatt taattaaatt
    11101 taagatttat ttaaaaaaac aaagataatc cttacagaat ataactcaca atttgtaagc
    11161 aatatacata taaggattat ccagattatc ttgtattata aaattttatt caaacagact
    11221 ttttaataac tgacaaatac acactagcaa taacttatca aagggttaat aattatgtta
    11281 ctaactaata agacttattc aatcagtaat atttatatag tttgtatcta ttaagctttt
    11341 atagcttcta ataaatcatc aacgctatct aatttttcta taagcattgg aactatttca
    11401 tgtaagtctc caactactcc atagtcagcg atttctaaaa ttggagctgc tgggtttttg
    11461 tttatagcaa ttatgaaatc tgatgattgc atacctgcta aatgttgtat tgctcctgat
    11521 ataccacaag ctatatatag gtttggttta acagtagttc ctgtttgacc tacttggtga
    11581 gaatggtcta tccatccagc atcaacagcc gcacgagaag aaccaactac tccacctaat
    11641 ttgtcagcta atttcttaag catttcaaat ccttctggtc cacctaatcc tagaccacct
    11701 gatactataa catttgcatc tgtaagagat actaaatctt tcttagtttt aactgtttct
    11761 aaaacagtag ttcttatatc atcttgagtt attttgtagt ctaatgctat aacttcacca
    11821 gttcttgttt cgtctttttc agctttatcc ataacaccag gtctaactgt agacatttga
    11881 ggtctatggt ttggacaaat gattgtagcc attatgtttc caccaaatgc tggacgagtt
    11941 tgttttattt tcttatcttc tgggtctatt tctaacttag tacagtcagc tgttaatcca
    12001 gttccaactc ttgaagctat tctaggtgct aagtctctac ctatatgagt tgctccgaaa
    12061 agcattattt ctggttttat ttcatctatt gcatctttta ttacttttgt atatgcatca
    12121 gttgtatatt tttctaaaag agcatcatct gcaacataaa ctttgtcagc tccaaaagtg
    12181 attaattctt tagctaatcc atcaacatct tttcctaata atattgcaca aagttctgca
    12241 tctacttctt tagctaattt tcttccttct cctaataatt ctataactac tggagctatt
    12301 tttccttctc tttgttctgc aaatatccat acgtttttat aagaacttaa atcttttata
    12361 tcattcatag tttgtactcc tccaacttat ataatgtgct tttgttttag accgattatt
    12421 attttactag ctatttcatc agctttacct tctaagattt ctccttttcc tcttggttca
    12481 ggagtaaatg atctaaatac tttagttggt gatgctttta aaccaacttc ttctttattt
    12541 acatctaaat catcaattgt ccaaactttt acatcatgtt tatatgcttt tactatttta
    12601 tcaacagaca tgtatctagg tgtatttaac tcttttactg ctgttaaaag tacaggtgta
    12661 cttactttta ttaattcgta tccatcttct aattgtcttt gaactattat atcttttcct
    12721 tctattttga aatcttgtac atatgtaact tgaggaatgt ctaatttctc tgcaatttgt
    12781 ggtccaactt gtgcagtatc tccatctatt gcttgtcttc ctgcaaatat tatgtcatag
    12841 tcacctactt tagatattcc cgctgcaata gtatttgatg tagcccaagt atcagctcct
    12901 ccaaatgctc tatcacttaa taatattgca tcatcagcac ccatagctaa acactctctt
    12961 agcataaagt ctgcttgagg aggtcccatt gttataactg ttatagttac atcatcatgc
    13021 tcatctttaa tttttaaagc ttcctctaat gcatttgcat catctgggtt taatatactt
    13081 ggaactccat ctcttataag agtacctgtt tctttattta ttcttacttc gttagtatct
    13141 ggaacttgtt ttacgcaaac taatattttc aaagtctata cctccaattt cattagatta
    13201 tttattattt taatacgttt gatgaaatta ccattttttg aacttctgat gttccttcgt
    13261 atattgatac gattctagca tctctgtaca ttctttcgat ttcataatct tttataaatc
    13321 catatccacc gtgaagttgt aatgctttat aagcaacttc atttgcaatt tctgatgcat
    13381 agtatttagc catagcagat tcttgagcaa ctctcattcc tgcatctctc ttttgtgctg
    13441 catcataaac taatcctcta gctgcacaaa cttttgtttc catgtcagct atagtaaatt
    13501 gagtattttg gaatttagct ataggcttac caaattgaac tctttgtttt gtatatttaa
    13561 cagcttcatc taaagcacct tgagctattc ctagagctaa ggcagcaaca cctattctac
    13621 caacttctag agtacccatt gctatcttaa atccttgacc ttcttttcca agtaagtttt
    13681 cttttggaac tttaacgttt tcaaatatta agtcagaagt ttcagttcct cttataccca
    13741 tcttatcttc atgagctcct gttgagaaac catcccattt actttctact ataaacgctg
    13801 atgttcctct aagtccttta cttctatctg taactgctat tactatagct acatcacaga
    13861 atggtccatt agttatgaaa gtttttctac catttaatat atagtggtct ccaactaatt
    13921 ctgctgttgt ttgttgtcca cctgcatcag aaccagctcc tggctcagtt aaaccaaatg
    13981 ctcctattaa ttcacctgtt gctatacctt ttaagtattt ttgtttttgc tcttcagtac
    14041 cattgtaaag tattggtaat gaagcaagag aagaaccagc tgtaacaaaa gtagctgttg
    14101 aagcacatct tctagctatt tcttccatta ctatagcgta agaaacatat cctgctcccc
    14161 caccaccata ttcttctggg atattactgc ttataaattt tgcatcagct aattttttga
    14221 ttaattcctt aggcattaca cctgttttat ccatttctgc tggtaaagta tctaattctt
    14281 tactaacaaa atctcttact gcttttctta aaagttcttg ttctttgtta tataacatag
    14341 cttcctccaa ttttcatatt ttatagtaaa tttattttaa tgaacttacg ctaaactcat
    14401 catctcagca aaagtttgaa tacgagttct ggcttgttca ttattctgag tttgttggtc
    14461 gatttcaaca tataaagtag gtattccact atcttctata tcttttctaa ctaaaggata
    14521 atcgtattct tctgggtcac agaatttcat catacagaag ataacaccat ctatatcttt
    14581 ctttttaact tcatctacta taagtgaccc acgttttttc ttagggtcat aagctaatga
    14641 acatccttct atgtttgacc attgtcttgc taatctctct aacgcatcat cacctgctgg
    14701 tacatctgtt ctgaattgtc ttgtttcttg tgctaagtcg tcagctacaa ctgatatatt
    14761 gttgtcttct aaaatgtcta atatatcttt agaatcagct aatatacctg ttaataaaac
    14821 tttctttcca gaacagactt cttctggcat agcatttaat tttgctatta aatctttcac
    14881 tagctcagta tgttcttctt ttctcataaa gaacccactc ttaattacta agcttcttat
    14941 tgatggtttt atagtattag aatgtttata agctacttca acaaagtctc tcatagtttt
    15001 tctatgttca ttgtaaactt ctatactttc atgaattttt gcttcttcta tttcatatcc
    15061 acaaatttct tcaagttctc tttttacgcc tttgtactca ctgattaagt attttacacc
    15121 agcttcaagt tttctatttt gtgggtgtac taatgatata tattttatat gaggtactgc
    15181 tgatttccag ttttgtccta aacaaattag tgtatcacac atacctggta taataactcc
    15241 agataattca tcataagcac cttttaatcc atattctaaa catgattgca ttattgaaca
    15301 tgcaaatgca gggaaatatt gtttagctaa atctaattct gtttgtcctc cccatatacc
    15361 aactggaagc attccagctg catgtataat ttcttctggg caataaactg ggaaacaacc
    15421 tatagctttt ttaccagttt cacttttata tttttttaca gccgcatttg gattttcaac
    15481 tacttctttc attttagata aaatagcttc cattatatct cacctctatt aagttttttt
    15541 ctttcttcca ttacttcaac taaaccttga attcttgttt caaattgagc attagtgaaa
    15601 gctctagggt ctgcttggtc accatcaaat ccagcatatg gtaatccagt ttcttcagct
    15661 gctcttcttt gcatttcata ttgtattaaa ctcataagtt tacagcttct gttcatatga
    15721 tagaatgctc catcacattt accctctact aaagaatcaa ctctatattt tgtcatacga
    15781 tctaggttta cattgttaaa catagtacta tatgctactg ccattccatc taaatcatta
    15841 acttcatatt gtaatgccca agcatgtggg taaacactac ctgtcatgtt aactccaaat
    15901 ttagctaatg ttttcatctt gtatcctata tatggccaac aaggtatacc ttccatcatt
    15961 attctgtatt tttcttcccc tctgaaagaa gatttaccag ttttcatgtt gtcctctaat
    16021 tcttctataa gtaacttaaa tgcttctgta gtttcttttt tacctctagc acaaactatt
    16081 acagccatgt aagtaaataa gtcaaatcca ttcattggag aaggagaaga atctgctggt
    16141 aaactcatag aatacttcca tagtcttccg ttttcagctg atattttcat tacttcttca
    16201 aacttcttag ggtcaaattt ctttcctgat ataatttcta gttgttttat agcttcttca
    16261 aattgagctt taatataatc tattcttgat tgagtaactt cgtcttcatt attgaaagtt
    16321 gtatcaatca ttattaaagg tatatctaat tctcttgaaa tattttcata ccattttata
    16381 acttggttac atatattgtt acagcaaagt aggaaatctg gagctggcat atccaaagct
    16441 tcacatccac cattttctaa aagaccaaaa tttgttcttg catatgcaca taggtcaata
    16501 gaatatcctt tagattcagc tatttcacat aattctaaag aacctttttt agctgctact
    16561 ccagctgctt ggttttctgg atataatacg tttaagtcaa atacttctgc taactcttga
    16621 ggaaatactg atgttgacca acctacaggt cttccttctt ctttagcttt aaatgcattt
    16681 gcatattgtt cagctaataa atcattaatt actactctag cttctttttt ttcagacatt
    16741 ttcatccctc tttttatata tttttcactt ctttttgtga ttcttttgct tcatcaaaag
    16801 catataacgc tgctcctaaa gcaccagtta attgaggtat atcaggtaca ataatttctg
    16861 tgttgatttc tcttgccata gctcttacaa taccactatt tctagcaact ccaccaacca
    16921 taactacatt tctttgtact cctattcttt ttactaggct agaaactctc tttgctactg
    16981 aagtatgaat acctgccact atatcttcaa tttttgcatt ttcagataaa tgtgatataa
    17041 cctcagactc tgcaaatact gtacatgtac tgcttattga tacttcattt tgagaattca
    17101 tagatatact tccgagttca gatacatcaa cctctattat ttttgccatt acatctaaaa
    17161 atcttcctgt acctgcagca catttgtcat tcataagaaa gtttaatagt cttccattat
    17221 tatctaattt taataccttt gcatcttgac cacctatgtc aataatggtt cttgtctctg
    17281 gaattatgaa atttacccct ctagcatgac agcttaattc acttatttgc ttatcagcat
    17341 ctgaatagtt cattcttcca tatcctgtaa ctacaacttt tttaatatct tctcttgcaa
    17401 gacctgtctt gccatataat ttttctaaaa ctcttgatgg tccagtagtc ccagtaccag
    17461 aggatattgt ttcagaagct acaatatctt ccccattctt taagattact ccctttgatg
    17521 cagttgaacc tatatctaat cccattgtgt acatctttag cattctcctt ttaaaaaatc
    17581 tagaattact aattaatatc ttacaacttt actatcttta aagttattta ttttctcttc
    17641 atcgtagcct aaagatttta atacttctac agtatgttga cctacttttg gtgctggtgt
    17701 atattcttta attccttcat ttctaaacat aactggagta ttaactaaga cacctgtatt
    17761 tccgctatcg tatgttttct taaataagaa gtcatttgcc caagcttgtt cgtcatctaa
    17821 taaatcttca cagctttgaa ttttttcaaa tggtaagtct gcttcttcta ataaagctga
    17881 ccactcgtct aatgtttttt ctaacatagc ttctccaact atcttaacta aatcttcaac
    17941 atgattaacc attgaatcta tgttattata tctatcatct tctaatatat attctctatt
    18001 tataacctta cagaatttgc ctaaccactt gttgtattgt attaaagcta attgaatcca
    18061 tcttccatct ttacatttat atgtagtcat taatgggctg tttggatttt ctcttgataa
    18121 aggcatttca tttccgtatt gtgctgttgt tatcattgtt cccattccat atatagctgt
    18181 atggaaaaga cttactgtta ctctctcacc tttaccagtt tgagcttttt tatgtaatgc
    18241 tgctaaactt cctgctgcta gtgctagacc tgcatagtgg tcaccaaatc ctgctgctgt
    18301 atttgctgga gatgttcctt tttccataac agattggcta actcctcctc ttgcgaagta
    18361 tgcagtatag tcaaatcctg gtttatcttt taaaggtcct ttttcaccat atcctaatat
    18421 ttgagagaat attaatcctg gatacttatc ttttatttgg tcataagcta tacccatttt
    18481 ttctaatgct tgaactctaa cattagttac aaatatgtct gcttctgata ataatttatg
    18541 taatatttct actccttctt ttgattttac attaatactt acaccctttt tatttccatt
    18601 ttctaattca aacatagggt tttcatcatc tgatgctgga gatttaaatg ttccacccat
    18661 aactcttatt ccatcacctt ctataggttc aatcttaata acctctgcac cccagtcacc
    18721 taacattttt gcacaacatg gtgctgcgat gaaacttgaa agttctacta ctttaactcc
    18781 ttctaaaagc ataacgtttt cctccttaat agtatcaatt ataaaatttc gattaaatca
    18841 ctcataaaat acttttttct gatattaact gtctgatgtc aaagttccta atcttatcta
    18901 aaataactta aaaattatta aaattatttt ttattgtttt attgtcttac acctatattg
    18961 ttaagcattt atcgtgccaa ctttatattt aatcaaaata tcgcttgatt tgaatgtttt
    19021 ttaacaatat gcaattgaat aatctttttc atatcgtggc atcttgccac tatccattgc
    19081 attttgctat tctttctatg cttataaaag ttttttttat atattttttt atgaaaaagc
    19141 ttgacatttc aaaaaaagtt ttcattgcta aactcaaaaa cacatgttat cattgtaatt
    19201 aatcagcaag ttagttaact aatttcaaac ttagataatt ttttaataat atttaatcta
    19261 aaaaataaca atttctttga taaattttag agtaattatt acattaaata aaaaattact
    19321 aaatatcaaa ataacacagg gaaaacaatt tcaaaactaa atttttaacc ttaaaaattt
    19381 tgtataaaaa atataacctt atatgctaca tattatttct aaatgaaaaa ttaccataat
    19441 aatttaataa ttacaggagg gtattgatat gaaaatacta gtatttggag cacgcgatta
    19501 tgaagaacca gtaataaaaa aatggtctga agaacataag gatgttcaag tggatattta
    19561 tcctgaaaac atgactgaag aaaatgtagt taaagctaaa gggtatgatg gtatatctat
    19621 acaacaaact aactatatag ataatcctta tatttatgaa actttaaaag atgctggggt
    19681 taaagttata gcttcaagaa ctgcaggggt tgacatgata cattttgatt tagttaatga
    19741 aaatggactt atcgttacaa acgttccttc ttattcacct aatgcaatag ctgaattagc
    19801 tgttactcaa gctatgaacc ttttaagaaa gactcctcta gtaaagaaaa aagtctgtga
    19861 aggtgattac cgttggatag ctgaacttct tggaacagaa gttagatcta ttacagttgg
    19921 tgttataggt acaggaaaaa taggtgctac ttctgcaaaa ttattcaaag gcctaggagc
    19981 taatgtaatt gcatttgacc aatatccaaa tagtgattta aatgatatat taacttacaa
    20041 agattcttta gaagaccttc taaaagaagc tgaccttata acattacata ctcctttact
    20101 tgaaggaaca aaacatatga taaataaaga tactctagct ataatgaagg atggagctta
    20161 catagtaaat actggccgtg gtggtttaat taatacaggg gatttaatag aagcactaga
    20221 gtcaggaaaa attagagctg ctgcccttga tacatttgaa actgaaggat tgttcttaaa
    20281 caaaaaaatg aatcctggag aattaactga cccagaaata aataaacttc tttctatgga
    20341 acaagttata ttcactcatc accttggttt cttcactagt acagcgattg aaaatatagt
    20401 ttattctagt ttaagcagtg ctgtagaagt tataaaaaca ggaactgcta ctaatagagt
    20461 aaattagtat tttataaata tattttttca ttaattagct attatctaac aaaatagcct
    20521 ttaatttccc aaatataaga gagtcagctc agtatatatt gagtagactc tcttatatta
    20581 ttttttatat taaacttgta ctcttcttat tcataccaaa ttaaatttat attaacttat
    20641 tctaactact cctactctat acattttacc tatttttact tttctaatta tatacatttg
    20701 aatgggtaca atctgtattt tacaaaaaaa acacctaaca aactatgaaa attgtatgat
    20761 taatattaat atatttataa gaaaacataa caattatgtt agctctggat ttttagggag
    20821 ggagtatatt ggaaaaaatg aataaagtta cttctaaaaa aactacctat attgttactt
    20881 cagcattatt tgcatccata atatgcctga caatagcata tatattacat attccagttg
    20941 gtggaaacaa tggatatgtt catataggag atgcttttat ttatctagct gcaactattt
    21001 tgccaacaaa ctatgctata gctgcttctg caattggtgc tggtctagct gatttatcta
    21061 caggtgctgc tatctgggta ataccaacta taatcataaa accaatactt gtattatttt
    21121 tcacttctaa aagtgacaag ataataaata agagaaatat agttgcttcg gttgtagcag
    21181 gtatagtagg attagtttta tacatgtttg ctgaaggtat aattattgga agttttacaa
    21241 gtgcatttgt aatgagctta cttggcttat tacagccaat aggaagtttt attgttttta
    21301 taatcttagg tatggcactt gataaacttg attttaaaaa acgttatttt aattaagaat
    21361 ttaaatttat ggagggaaaa ttatgaatat cttatataca attgaaaata gtatctatgt
    21421 aaatataact aatacatgtc catgtagttg tgttttttgt ataagaaacg aaaaagatga
    21481 agttgcaaat agtggtagtt tatggctgga acatgagcca agtgtagatg aagtaaaaga
    21541 agccttcaat aaatacaatt tagacgacta tgatgaaatc gtattttgtg gatatggcga
    21601 accattaatg agaataaatg aattgataga ggtagcaaag tttataaaag aaaaaagtag
    21661 cataaagata agaataaata caaatggtct aagtgattta atacataaca aaaaaacagc
    21721 tattctgtta aaaaatgtta tagatgcagt atcaataagt cttaatgccc caaataaaga
    21781 agcttataat agagttactc aaccaaagtt tggagaaaaa tcatttgatt atatgttaga
    21841 ttttgcaaaa gattgcaaaa aatatataaa agaagtagct ttttcagtag tagatgaaat
    21901 atctccagaa gaaatagaag aatctaaaca actggcaaaa aaacttgata taccattaag
    21961 ggttagacat aagaattagc aagtaaaatt ataaaacatt aattataaaa cattaagaat
    22021 cacctgaatg gtctttcagg tgattcttcc tcttgtatta acattttaca aaatagattt
    22081 cataccacac taggaatgtg taaatcatcc aaatctttct ccaattatca gagtttccac
    22141 taatgtagtc attaaaaatc tcattaatct catctatgtt aaagaattta tctgctatct
    22201 cactattgaa tttatctcgg atgtccttat tatattggtc atcagccatc catatacgaa
    22261 ttggcacaat aaatcctaat ttatttctaa aagcaatctc ttctggtaat gtttttgatg
    22321 cagctgtacg aaaggcaatc ttattctctt ttcctttcac tttatattct gatggtattc
    22381 tggatgcaat atcaaatatt cttgtatctg taagaggcat tctgacttcc aatccaactg
    22441 cagtactcat tttatctata ttaaaataaa tatctccttc caaccatatt tgaatatcta
    22501 catttgacat gttagtaagt gtatctagtt cttctgtttc ttcataaatt ccctttacca
    22561 cttcaatagg aagcacatca ggattgtatt tcttaagaat ttttcttttc tcaccctcat
    22621 tcataatgtt tgtatttcca atataaaatg tcttcaaatt ctctccataa cactctgcct
    22681 tatggtatat attatatcca ccaaaaaact catctgctcc ttctccagag taacagatat
    22741 ttgtgttttc agctgctgcc ttgcatccaa tagcaaaaac gatagcagat gcatctccag
    22801 ttggttgctc catatggtac attacataag gtactgacgc aaaatactct tcaggtgtaa
    22861 ttatatatct cgaattttta cacccaagaa tattagctgt ctgttgtgct aattttgatt
    22921 catcaaaacg ctcttcctca taaccacatg aacctgtttt ttctatatct gacattgcca
    22981 atatataaga tgaatcaaca cctcctgata ggaataactc tgcagtttca ttctctgatt
    23041 ttacctctgg cataattttc tgaagtgttg tatgaatttc atccgtccat tcctcaatag
    23101 acttgctgtg gtcaggatga aattcaggtt tccaataacg accaatacat actgttttat
    23161 tctgatagac aaggtaatgt cctggcataa gcttctttac tccacggaag aatgtatttt
    23221 cacctgctac atatgtgaaa gacatataaa tctgtaacat gtcttcattt aactctttca
    23281 caaatcctgg ttgctccata atcttacgaa tagatgaacc atagagaagc ttcccatttt
    23341 cagtctcata ataatagaaa ggttttactc caaactggtc tcttaggcag aagagtttct
    23401 tttccacagt atcccataat gcaaaagcaa acattccatg catgtgattt gccatatcat
    23461 atccccatgt cttatatgct tccactaaaa ttgctcgttc acgctcatct ctacataatg
    23521 aagaatcgat tccaagttct tcacagagaa ctttccagtt tcgaatatgt ccattgtatt
    23581 ctagaatttt tatcatatat gtgtcttttc gtgttaaata cttttcttct aaatttatca
    23641 tatgtgtatc ctttcgtatt aaaacattta acttctaaat ttattaaatg tttatatata
    23701 tttattcact tataatcact gaaaaatcga cttttttcaa agtaaattct ttttgaatta
    23761 gtactaatct gctctttatt aatggcatca atatgattat caagtattat cagtgttata
    23821 acactgaatt ttttattctt cataaatatt gctcccttta tattaccaac taaatataat
    23881 atatcttatg ttacttacat attctttaag aaattctgaa gattttataa agttttttgt
    23941 attataatca attaaaaata aaattctagt tataaagcat attttccatt aaacaaaaaa
    24001 atagttaaaa taaattcata actacataaa cttattttaa ctatttgtat attttaatta
    24061 taaatattaa cttaaatttt taacaggata cactcataaa ataaacataa tgaacaacaa
    24121 aataatatgt ctcagtttct ttatacactt taccttcttt caattttcta ccatgtagac
    24181 gtacaagata atattagtaa ttcactctat tgctgtaatt tactctattg ctgtaattca
    24241 ctctattgct taactcgtgc gatatggaca accaagaaag ctatttcctc atttgttaac
    24301 ttttttccat atttcttttc taaaaaatct ctaactttta atgcaattaa catttctttt
    24361 ggatttgacc ttttcatttg attaaataat aaatcatcac cactgcttaa catactatct
    24421 gaaaaaaatc tttccacaaa aaattttata tgtgttataa atctcatata atgcatactc
    24481 tttttatcta aggttctatt caaagaaaaa acaaaaatat taattatttc tccaattact
    24541 ttggcatact taatcgtatc atatgaattt ccatctgaag ccatagcatt tgcaaaatgg
    24601 aaagcaatgt tagctgcttc ttcctctggt aattgaatgc ccaatctctc ctctaccata
    24661 taaattgcaa ccattccaac cttaaactca tttggataat aattttttat ttcccaaaat
    24721 actcgatttg taatttttat tcctttacgc atcctttcaa ttgcaaagtt aaaatggtct
    24781 gctaatatta agtataagtt tttattaaaa gatgagttca ataacttttc tgcctctact
    24841 attatttcct gtgttatctt taaaatgtcg acaggaatat catccattaa ttctaaaaat
    24901 tgttttgatt ttgtattttc aacagggaca aacatttgat tatcattgga accacaaata
    24961 tattctcctg tttttttacc atatccaatt cctttcccta gcaaaataaa ttcattatcc
    25021 tttgtgtcat gaactaaaac aacgctcgag ttcaaaactt tctttattat gtagttcatt
    25081 ttatttgctc accttctatt ggtcatttaa aatctatact attattttca attacttttt
    25141 tataccaata aaaactatct ttcttatatc gattatatgt cccctttcca taatcatcag
    25201 catccacata aatatatcca taacgttttg acatttgttt cgttgattca ctgaccaaat
    25261 caatacaacc ccatgaagtg tatccccaaa gttcaacacc atcttcaata atcgcatcta
    25321 acatacattt aaagtgttct tttaaataat taattctata ttggtcatga acttttttat
    25381 cttcagttaa aatatcttta gctcccaatc cattttcaac tataaataaa ggctttctgt
    25441 atctatcata taattcaatc aaagatatac gtagacctat tgaatcaatt tgccaacccc
    25501 attcactcat ttctaaataa ggatttttta cagctaataa cgtattccca ggtgtaatat
    25561 ctaatccagt tgtatcagct gcctcacaag aagacatata gtaagagaat gaaataaaat
    25621 ctactggata ctttttcata atttcatcat catgttcttc ttttactatt tgaatgttat
    25681 tatttttaaa atacgacagt aaatatactg gatactcacc aaaaacctgc gtatcactat
    25741 atgcataaat acttctcatt ttttgttgag ttgccaaaac atcttctggt ctacatgtgt
    25801 atggataata cgttaatttt gttaacatac accctacttt tgaatttgga ataatttcat
    25861 gacaaatttt tgttgcaagg gcactagcta caaattgatg atgcatagct tgaaaaataa
    25921 cttcctcaaa gtttttatcc ttaaaacggt ctcgaactaa accaccagta gtgtacggat
    25981 gacgaatcat agaatcaact tcattaaatg taagccaata tttcactttg tttttatatc
    26041 tttcacagat agtttctaca tacctaacaa acatattgat aacttgtcgt gaataccatc
    26101 catcatagtt taaaacgata tttaatggtg gttcataatg tgacatagta acaagtggtt
    26161 caattccata acttatcaac tcatcaaata catcatcata aaactgtaaa ccttcttcat
    26221 ttggctcttt atcatctcca tttggaaaaa tacgagacca agcaatactc attcgatatg
    26281 tcttaaatcc catttctgcc attagagcaa tatcttcttt atagtgatga taaaaatcac
    26341 taccatgacg ttttggataa tttacttcat cttcaggatg tgctaatcct tcttctatat
    26401 cttttgttgt gatttcatta ttagatttat aatctttcac atctgcatca aagtgtgcct
    26461 ttgctacatc tgatacagac caccctttcc cacctaaatt ccaaccacct tcaaattgat
    26521 tagcagctgt agctcctccc caaagaaatc cttctggaaa ctttctcata tttatattcc
    26581 tccctttaca tcaaacaaga tacttccttt ttttacattg ccataagact ggtttaacac
    26641 tttataagca taatctgctc cattattgtt tacaatcata acagttgaat ctaaattagc
    26701 atgtgagatt ttgtctaaat caaattgaat taataaatct ccttctttta cattatcgcc
    26761 tgtcttaaca aatgttttaa atccatctcc attcatactt acagtattta ttccaatatg
    26821 aatcaaaatc tcagccccat tttttgttct taaaccaatt gcatgttttg tttcaaataa
    26881 catcacaact tctgaatcac aaggtgcgta taaatttcca ttggttggta taattgcaac
    26941 tccatctccc aaagtcttac ttgcaaatag ttcatcatta accttactta aatctattac
    27001 ttttccttct actggagtta ctaagatatt tacttctttt tcttcttcta gctcttcttc
    27061 aacaacatct ccaattgatg actctactgt atttgtatct tccttccaaa taatcagtgt
    27121 aataataaac gagcctaata ttgcaataac aaaaccaata cttgcaaaaa tcaaattgtt
    27181 tccattgttt gggtcaataa acatactcat actggcaagc cctggtccaa caattacaaa
    27241 tccttttact gcaaataatc ctaaaaatgt accagaaatt gcacttgaga atacaacacc
    27301 taacattgct cttttatgtt gtaaagtaat accatataaa gctggctctg taattcccat
    27361 caaggcagaa attcctgcag ataatgctgt ttgttttaat gtttcatctt ttgttcgcaa
    27421 ggctactgca aaacaagcac cactttcaga aatattgtgg gctaatgaag atgtcatata
    27481 taaagcttca taacccaact ttccaattgt attaattgaa taaggtatta gtgctttgtg
    27541 cattcctgtt gcaaccataa atggaagaaa agcagcaagt aatcctacaa ctaagaatcc
    27601 cattttgtta tataagaata aaataatggt tgtaaaaatt gtaccaacgt tatatcctaa
    27661 aggacctaaa atagttagag taattggaac agtaattgcc aaagacatca taggaacaaa
    27721 gaaagttcga attggttttg gtgaatactt gttaaaaatt ttctccattg ttgccaagaa
    27781 agacacacat aagatagctg ggaaaacctg tgcattatag gcaatgtttg gaattgtaaa
    27841 tccaaatagt tcgacacctt cacttaaagc agttgttgtt gctggaagca ataagtatcc
    27901 tacagcggca atagctacta aacgattaca tttcaataca ttggcagtag ttacagctac
    27961 cataataggt aggaaataga atgttgcatc tgaaatagca actaataatg tatatatagt
    28021 agaatctgat tttaaaatat taaacattgc taataacatc aacagtgatt ttaaaatacc
    28081 agctcctgca atagcaaaaa tcagtggttg aaaaatatta ataataaatt ctataaatct
    28141 ttttcctaag tttatttttt cttcagtttt tacagtacta tcatttgtat ctaaagggca
    28201 aacttttaaa atttcatcat acacatcaac aacactattc ccaatgacaa cttgaaattg
    28261 tgccccatta actatactca taacaccttg tacttttttt aaagcatcct gattgacttt
    28321 acttctatca atcaacttaa agcgtagtct agttgaacag tgttctaaag ctaaaatatt
    28381 ttttgaacca ccaacttgtt caataatgtc aatggcagtt tttttataat tcatcttgta
    28441 atctccttct ccataatttt ttatgaatgg ccattcaaga tgcaaaaaag gcagaaccta
    28501 tataaacacc ttgaaaaatc aaagcaatat attcgatcct gcctgcatta ccagtaacat
    28561 gtcttctatt tatactattt tgtattatta tattaacatt tgcctaaaag aattgtcaat
    28621 acttttttaa attttctgat actaactaat agcaccttta taattcattt tttatgtcaa
    28681 ttaaagtaac agtagccacc caccaattag tatcaaaatt gaacctggta ttttttcttt
    28741 tatattttct ttgaatataa aatgagcaag tattaaagtt attatcatag atacttgaga
    28801 aaatggctct gctatactaa gttctagatt atttaaagca attaataatg ctaaataaga
    28861 atatccatta atagcaccac ttaaaattga aagccccttt ttattagtaa aaatctcaca
    28921 aattattttt tgtttctttt taaatgctaa aaagataaat aaatttaaac ctacgaaaaa
    28981 atacagtata gttgaatata atataggaga tacttgtaca aaaaaagcct tatccaatac
    29041 tcttcctact gactgcaaaa atatgtataa aaacattaat ctacatggta aattgttaaa
    29101 aattacagaa acattcttta aaaaagagct tacttttttt aatataaaca ctccaccaat
    29161 cataattatg attcctgcaa cttttgctaa acttaaacct tcacctaaaa atataactga
    29221 taaaagcagt aaaaacaatg aatttaaact atttattgga gtaacaagtg aaacttcccc
    29281 agttgctaaa gacgttacat acgctactga accaattgaa tagactaaac tacttatata
    29341 acatggtatt aaaaacgacc aatcactaac tctatccatt atgctaaatg gaagtaaaca
    29401 tactccccct ataaagaaaa atagaaatga tgaagctata tttttataaa tatctccttc
    29461 ttcatcacct aataatctta caactactct ttcaaaagct aatagcacta ctctaattaa
    29521 taatgcaaaa taagataata tcatttttga cctcgcttaa aaattaatcc gacttattgt
    29581 tatattaact gtataagact accatatagg taagtctttt ggatttttta atattttatg
    29641 tttaaataca ggtacattaa gtttttcacc tagtttacgg cattgctctt tataggtacc
    29701 ttttaattta tcatgaaaaa ctatacaatc aattcctcct tcaatactat ttatacactt
    29761 ttctagtttc tcaaatccag attcatcatc atacattcca cttacaatcc attgatttgg
    29821 caatacatgt ccaattgata tgttatattc tttatttttt ataatatctg agaaagattt
    29881 ctcaaaatgt acccctccaa cacataacaa agtcttcaaa ggttcatcac aatcaaatac
    29941 tctaattaat gattttgcca atacctgtat tgctatacta ttattaaaac tatcactact
    30001 acttcctatc tcaatatcca acataggtac agaatattct gtaattaatt tagattcttc
    30061 accatatgtt gttccagacc agtgagttgc ttctgttaat gtagtaaatt catcaagtag
    30121 attttcattt tttatatcct caatagcatt gattagattt ttaaagtatc ttggatttga
    30181 atttccaaat tctcctgttg gaacatctcc aattgtatga gctgttagga tatgttctgg
    30241 agcattttct ccctcatgcc aatttacaac tcctgcaaaa tcataatctc taaaatgtct
    30301 atttaaaatt ggaagataat atttataatt atgacttatt acatcttttg tttttacaaa
    30361 atgaaataag ttattgtttt tatccttaaa ctctaataca aaattatcat caataacaat
    30421 atcagtttga tttaaatcca ttatttcttc acaacatttt atcacattac tggctactgg
    30481 gtctttcttt aaatcactgc aaaagaaaaa aactgctttt ttattattca tccataaatc
    30541 acactcctat aacttaaata taatatatct tcatacctaa aatcatctaa atatcactct
    30601 aattccatta aattatttat atataatata ctaccatact cattttagct atacctttgc
    30661 ctgagtttct atatatatgt gatacatcag ctttaaatct tatagaattt ccactagata
    30721 caatgtaatc ttctccattt attttaatgg ttacttcgcc actaaaaaca gttataaact
    30781 cttgtgttcc tggaagttca gcatttgaat ctaaatttcc accttcttct atttcaatca
    30841 taaatatctc aaatcttctt ttggagtcaa atggaaatat tggataactt atatactttc
    30901 cattatcttc aataaaatgg tctatatcac ttttttgaat caattctatg tcactttcct
    30961 gcctctccat caatgatgta aatgagattt ttagcccatt agttattttc ttcaaagtag
    31021 atatagtagg atttacctca cacttttcaa tttgagatag catactctta cttacaccac
    31081 taagttgtgc aatcttgtct aaactaagat gtttttcttt ccttatcttt tttaaatttt
    31141 cagcaattat ttcatttaaa ctttgcataa atacccccta atacatacta atataagata
    31201 aattattttg attattaaat aaacttagta atttaataga aactttttat ccattataat
    31261 gtacattatg tttaatatac attacttttt ttagaatgta aatagttttc tgaaattttg
    31321 tgaaaatgaa taaataaaaa actagtaacc tatattttta caaggttact agcttttatt
    31381 ataataatct ttcattttaa ctataaaact tagatgcacc tataagctgt ctcttagaaa
    31441 caatcttagc taggtttgct aatgaagaaa tttttatatc tttaaaagaa actaaaaata
    31501 caaacttaaa atctgtaaat tcttgattta tattgtatat atcaaaataa catctcaaat
    31561 tatcaaataa gtaattatcc tgtaagtctg cttctatctc atcagtagca tcttttaaat
    31621 acgctcttac atcttcaaat aaaacactga aataagactc atctatttct tcacctaact
    31681 cttccaatat ttcttctata tctaaatcat ccatatcaaa agaatttcct gtcaactggc
    31741 aatcaaatac caatatattt tcttgattta tagtatttga tatatcttgt acatattcga
    31801 agtctggacg taattctaaa atgtctttta tttcactgct ctttacatgg atttttcctt
    31861 ttctattttc aatttttaaa tctatcatat gctccccctt tcaatctaca aatctttaaa
    31921 taccttgttt tataatcttt tcaatatcta tccaattact aactctaact atattttgat
    31981 gtttaacatc tttattatag ttagtatcca gaagtattac tttaacacca tcatcagcta
    32041 gttgcactga gtttaaagga ttatcttcta taaatatgtc acaactcagt tctcttgcct
    32101 tttcaacttt ataatcactt cctaataagt aaacatctat atctgaaaat ccttgtctcg
    32161 atagccaatt tttagttaca tcagttaaac actcatgtct agccgttaca aaagataaat
    32221 tatgtccttt agataatgtg ctaattacct ctgtagcacc ctctacaatc tttgcttcat
    32281 catatgagtg tatatactta ttattaaact ccgcatacac atctcttatt cccgaaccat
    32341 acaattcttc ccaattatgt gttatacact ctgcttcagt tatattttta ttaaagatat
    32401 catttaggta tggaagaaaa tgataagggc ttgttattgt tccatctata tcaatacata
    32461 tattcaattt gttcattttt acctccatgt aacatttatt gatttgatta atcaaatatt
    32521 atagcacagc tttaattata aatcaaaact ctggatatga ctatacctat ataataccaa
    32581 aaagcttgca tatttaagca agcttttcgt aaattttaca tttttaacta atatataagt
    32641 ttttctaagg ttggttccac tccaaattgt gatttcagtt ctttatattc ttttaatgct
    32701 ttgtcattat tcgttttagt ttttatcgct cttatcatta aattttttgg tgtatcaagt
    32761 ggagatatgt attctactac agaagtatca tatccattgc cctccaataa caatgttctt
    32821 aaaccatcag ttattaaatc tgcaaatcta gctttaaata caccatgttt taatattggt
    32881 tccatagctt catatctata ttgccctaat aactccttat gacaacatgg tactactact
    32941 atagcttcag attttgctct aattcctaga cctattgcca tatctgtagc tgtatcacaa
    33001 gcatgtagac ttattacaag gtcaacatcc ctattaggtg tataattagt caaatcctct
    33061 tttataaatg acatattttt ataacccaag tttttagcca tattttttga tgcttctata
    33121 actacatctg aataatctat tcctataaaa taacagtttt tcttcaatac ttcttttata
    33181 taaaaattta agacaaagct taagtaagat tttccacaag cacaatccaa gatagttata
    33241 cagtctttat cctttatttc ttttaaaata ctatctatca attcaacaaa atggtctatc
    33301 tgattgtatt ttcttatttt atcatttttt atcttaccat cttttgtaag tactccaatt
    33361 tcttttaaaa gtgcatttgc ttgtcctact tttacataat aatctctgtt ttttatttga
    33421 gatgccgtaa attcatctat tttaggtact tcttccttat tatctaaata ttttacattt
    33481 accttcttat tgtctccttc aattacaaca atagttcctc gctcaacata tttaaatact
    33541 aatccatcat aattttctgc ctctttagaa atattcttaa gcaactcatt aaacgtatat
    33601 aaatctgatt ttcctttaaa attaaattta tatttattat cttcctctat tgcaattcct
    33661 ttaaactctt ttcttccaga tttaaaaatc acctctaact caataaaaaa atccctatta
    33721 tcaaggtttc tttgagtaat ccccataaaa aacatattta gttttcctat actctgttta
    33781 ttcatcataa tctccctttt aacttatatc cttaaatctt attaattaat attatttgtt
    33841 tctgaattat ttatttcttc ttttaaatcg ttcaatctat ttttaaaact ttctatctgc
    33901 ttatctgata aatcaagata ttctgttatc ttccctatat atttagggct tttagtattt
    33961 attaatttca aagcactttc catagatttt tctctaagct catatgcttt ctttacgtca
    34021 tctctagcat tactcaatgt ttctgtatac ttctccttag aaagttctgg aatctgcata
    34081 ttttcatatt cttttacaac ttctttacac ttgtttctgg catttgtaac atcttttttt
    34141 atactagata atttagttat attttttgct atatccttag tgtaaggtat gcattctctc
    34201 attggaacta aagctgctct ttctatatta taaatttctt tcttaaaaat tcctatactt
    34261 tccatatcta ttttttttat atcttcacta tttttttctt tacttgtata tttattattt
    34321 tcttttgtat tatcttcttg tttttctgat ttattattca ctgaaacatt actatcagaa
    34381 ttattctttt cttttatatc attacttcct tcttcattac tttctttatt ttgtatattt
    34441 ctatcttgtt tgttgtttaa atttttatta tttgatattt cttcattctt tttattatcc
    34501 tgttttaaat cagtactttc tttttcctta aaatttgata catacttttc atcatatgtc
    34561 tcatcagaaa tataattgtg ataatacata gactctttta ttttattaag tccactcata
    34621 taaatattac ttatcattat tgttgtagct aataccacat aaatactact gtagatacaa
    34681 gacctcttaa aattcttgta atttctgaat atcactacta aaggaataag atataaaaat
    34741 ataaaaacta atatccacct cataagtcgt gtctcctttc tcaaaaccga aatccctcaa
    34801 atatttattt ttaatcatgt atcttatagt atttaccaat ttatgttttt taatcgtgaa
    34861 cttttattaa attaaattct gtatagtcac aagatgctaa tgtatattct actccatttc
    34921 gaatcccctt caatcccatt ttaaaagatt ctttaccatg caagtgacca taaatcacct
    34981 tttcaacatt atattcttca aataactttg taaataaaga ttcttccaat ttatcattcg
    35041 taggaggata atgagtaatc acaattattt ttgagtgacc acttttacga gcagattcta
    35101 atgatagtct aagtctatgt tcttctcttt tataaacttt ttcatcagtc tcatcaaatt
    35161 tgacatcatt aggacatatc cagcccctac ctccacaaat tgcataatct ttatattcat
    35221 aaaaatttgt ctgaataaaa cgcatgtctt catatagctt gtttaaactt gtaacggttg
    35281 tccaccagta atcatgattt ccttttataa gtattttctg acctggaagt ttgcttatta
    35341 tatctaaatc ttttttagct tcggataaat taattcccca agatgtatct cctagtacta
    35401 agaccatatc ttcttcctta acaacttctt tccaattatc tataattttc ttttcatgac
    35461 catcccaatt acttccaaag atattcattg gcttattgac tgatgttgaa aaatgtaaat
    35521 cacctatagc atacaaactc ataatatcct tcctttctta attacactaa ataggctgtc
    35581 tctttttaga aattattttc cattaagagg cagccccatt aatatttagc aaattttcta
    35641 caagtagttt ttactgaact actttatcat ctaccttagt atccttatat tgctctaata
    35701 atatatctat ttcttgtttt aattccaaaa tttcagttat atttttatct gtttcagttc
    35761 tatcaagttt tctttggtac tgcatagctt tatgtgtatc acccaagtca tataactctt
    35821 gtattctatt tagagtatct ctaatagcta tagacttcat ttggtacata gcctgagctt
    35881 tcattatgtc ctctcttatt tcactcattt cttggtctaa aagaaaggca gcatcagatg
    35941 ctcttctaag cctttcagca agagtctcag gtatgtaatg gtcatattta tattttaatg
    36001 aatgttctat tgtagcccag aaattcattg caagagttct tatttgtatt tcacatataa
    36061 tttcctttga acccgcaatg gagtttatag ggtattttat tataacatga tagcttctat
    36121 aaccactatc tttgtaattt gtaatataat ctttttctcc tattatagtc atatcatttc
    36181 ttacttttat caaatcaact atagcatata tatcctcaac aaactggcac attattctta
    36241 taccagcaat atcatcaatt tcagtttcta tatcttttgc atttatcctt tttaacttag
    36301 atattatgga agatattttt tttgttctac ctgtgacaaa ctctatggga gaatattcac
    36361 cttttgctaa atattctttt cttatatttt taaattttat tttcaattcc tctactgcat
    36421 gttcataagg tgctagtatc tcattccatt tttcgtattc catattgctc accctttatt
    36481 tgttttttat gaccttcttt attttatcat aatttatttt gtttttgtat agtaaagcaa
    36541 ttctccattt tccaatatat accctatttc ttctaaatca actaagtaac ttatgacaga
    36601 tactaacgaa gatttaaaaa aatgatactc tttataatta ttgcttaaat tattgttatt
    36661 tataatgttt tttaataaaa tttctaaaga tttcggttct acaagcaact ctcttatttg
    36721 atataaatat ttttttaaag cattcaaatg tttttctatt aatttataag aatcttcttt
    36781 cgatataact tgcttgccat gccctaaaac taagtactca aaatctatat tttttaattt
    36841 ttctaatgaa tttatttgtt cttgtatatc atatagaaac aagaaatcgt attttgataa
    36901 aatttcatct cctatcaata aatcgccaac aaaaatcaca ttatccttcg tcaaaacagc
    36961 tatactacca ggtgtatgac cctgaaattc aattatatca aacatttcat tgtttaattc
    37021 aatttttcca gcttctatag atttatctat ttttatatca tccaacattt tattttttaa
    37081 gatattatcc ataaatacat tgctctttcc cccaacaata tactttgaaa acaattctgg
    37141 attttctata tataatttat cgtattcaga tgacattata cacacatctt tataatattc
    37201 tttaaattga ttacaagctc catagtggtc atcatgacca tgagtattta taatatatct
    37261 aacctgtata ttattttcat ctaatacttt atttactctc ctaggtcttg aaccacataa
    37321 ccctgggtct atcataagtg ctgtgttatc ttcaaataca tatattcctg tattcgtgcc
    37381 accttttata aaaaaggtat ttccttttac ttttgtaaga ttcaccttct tcacctcatt
    37441 ttgacaatat ttatgataca atttattact tactaatata aaactataca agaacaatcc
    37501 tttttattct atctatagtt ctttttctta ttttgtattc tttagatatc cttattttca
    37561 cattatatgc taaatttcta taaagtaatg actctataaa atattcaact atacctctat
    37621 ccctaaagtc tatatctata tttccacttg aataaatatt tgagtctaat atcataagct
    37681 catcaaaaaa gcttaatctc ttagatttac ttaaataatc tttatttgga ataaatctat
    37741 cacaataatc tatcctgtaa tttactactt catcatttat ttcaaatcca tattgctttt
    37801 tcattaactc tctatagtta ttatttttct caataacatt atctaaattt ttattataat
    37861 cctctttttt ccaatgtcca aacatcctac aatttaatgg tctaacttca tatattaaac
    37921 atctattatt agtatcttta aatggacatg ggttctttct tgtatactcc tcaaaataat
    37981 aatctaatat tttacttata cagctttttt ttaaatcaac tctatcttca agataacaaa
    38041 atatattcaa aaactcaatt aaatttatac ctactgactc catacaacaa tttccacatc
    38101 cagaacaatt tcctactggc aaggacttat atacatcatt tagtttgtca aagagcttat
    38161 ttttaatgga ataatctata caatttaaaa tatctttatt ttttatacta gtcattatta
    38221 ttactccttg ttataattat taagttaata caaacaatta ttataacata ttttttaaat
    38281 ttaatcgcat atggaggtat tttatgaata gatatacaaa aataattaat atgatgggaa
    38341 gttattacac aaaagacttt gaaaaagaaa aaaagaatgt aataaaagtt agagaagtta
    38401 aagaagatac agttagaaaa tttttcttac aaggtgactg tgaagttctt gtagtatttg
    38461 aagacactgg gaaagaaata cttattgatg acttttctcc tgaagaagat atcaaaaaat
    38521 atttaggagc aaaatttata aataaaaaaa gatagttatc aaaaataatt ctaaaattaa
    38581 aatttaataa atgtacagtt ataattctat ataattatag ttaaaagtta tcttttattg
    38641 cttttttaga ctttaagata gctttttttc tattttttgt aaaataattc cataattcac
    38701 attaataatt cacaaaatga tataatataa caaatagata aattttaaat ttctaaatac
    38761 ataattaata ttttgcgagg tgattttatg caaatttatt ttagagttaa ggctgttggc
    38821 aaacgcaaac ctatgctaga acttacccct tttgatgtac ctgacaacat caattgccta
    38881 aaagaagcta tctctgaaat tgtatctaaa aatgttcttg aatataatga gaaaatccca
    38941 gaaaaatcaa taataaactt cttaactaat gaagaaattg aatctcaagc tgatattgga
    39001 aaagttggat ttggctctat ttacaatgag aataaacaat ctgtagaaaa ggcaatagaa
    39061 gtagcgttac aggcatttga agatggaatc tacaaagtgc ttataaatga agatgttatt
    39121 gaaaaactcg attcctctat aaatctgaaa agtggagata tatttacttt tataagactt
    39181 accttgcttg ctggaagaat gttttaaaat aaataatttg gaggtacata aatgttagaa
    39241 cgtactagag tagaaaaaaa tcctaaaata attgaaaatt ttaaaaataa ttttactaaa
    39301 tcttcttcaa aacagtacta cgacttagca aagtctcttt gggactgcat agaaaatagt
    39361 tcatactatt atagagaacc tgaagacaaa ttcttaaata tgataaagaa atactacaat
    39421 tgccactact acgaaattaa aaatatggac gagctccttt ctggaccttt aaaaaatgca
    39481 ttagaattaa tatttacttc tgaacaagta gaagaacttt gccaattaat taaaagaaag
    39541 atggattata catattcagg aggatattat cgtaaagggt atcgctcaaa taaacttgtg
    39601 gattatttta gaggtattat aaggtttatt tcaaactatg ttgatttaat ttattatgat
    39661 ttttcagtta tagattatct tactcttcca aataaggtct tgtactatga ctctataatg
    39721 agtgataaaa tagcactaga aatagacaaa ggaaatcaaa aagtaataag tgctttagaa
    39781 gaagtaatat ttggagaaaa tcaaactgct ctacttacaa gacaaatgat aattggtatc
    39841 atgaagagtc atagtgataa atgctatgaa atgctttcta aacttctttt ggcagccaaa
    39901 caacaagaag gacttagaca gcaaattgta gagagtttag atgagggtac aatttctgca
    39961 tcaatataca tgttaaaaac tattttggac aacaatttag agcgatttag ttctgttata
    40021 agagccttgg atacatggac aggacttggt ttttcagacc aaaaacctgg aataatacgt
    40081 aaatgtttag aactttcata taaatcattg actgatgaaa agttccgtat agaatcaatt
    40141 aatagcaatg ataatcttga aatttatttt gccttgtggg ctactgcaac ttacaacata
    40201 tatgactctt actctcttat tgaaaactta ctttcttgtc aggaaaaata taaaaaactt
    40261 gtagctcttt actttttatc gcatacagaa agtacatatt ttaaacattc tatagcatct
    40321 aaaaacatta agggtgatga tttagagata ctagcatggg taattacaaa tttctatgta
    40381 gaaggttctg ttggctacta ctatagttgg caaaacagag aaactaattt tgacttgtca
    40441 aacactccta ttgacaaaga ctttgatgct agaaaagaac aatttgaaag tttaaaacat
    40501 actttagaaa ttttaaattc aaagacaaaa tcattttcag gaagtgtatt tccatggact
    40561 tggattgaaa tttcttctca tgaaatactt aaatgtatga tgactttaac agcatacgat
    40621 atgaatgctc aatttataga tgtgcttatg gaaaattgtc atcttatgag ttcagattta
    40681 agagcttcac ttatagtcaa ttttgtaaat gaaccaaaga cagatagaca acgtgaattt
    40741 ttattggaat gtttaagtga taagagtgtt tccaatagag aaacggtatt gagtctactt
    40801 agaaagcttg agctaacaga tgtagaatta gataagataa ccaatctatt gtctttaaag
    40861 acaagctcta ttagacaaac agtaataaaa attttacttg aacaagatga ttcaaactta
    40921 gaaaatattg cttcttcact gataaaatct tcaaattctc aaaaatgcct tggaggtctt
    40981 gaaattgtaa acttattaaa agacaatact gctagacaag aactatataa taagcttgtt
    41041 ttacttatag aagatattga taaatctaaa aagaatacat ctcaagttca aatactaatt
    41101 gataatttgt taggtaaagc aaaaaatgat tactgcaaag aaaatggttt tggaatatat
    41161 aaccctgaaa acaaagttca aattccagaa caatttgatt ttaaagatga ttctaatagc
    41221 tcatcaaagc ttaataaaat tatctcaaat ataagtttat ctactcgtga ggattctgtt
    41281 tctgtagcaa caaaaaaaga agcaaaaaaa attctcgata cggatataaa taaaataata
    41341 aaagtttatg aaaaatttga tgagcttatt actgaaaatg cagattatga atatgaagtt
    41401 gaaagatggt caggtaatgt agacaaagta cttttaggtt attctactta taacttgact
    41461 cctattaaat ataaagtttc taatgatact cttgataatt atccattagc agagctttgg
    41521 agagaaacta gtaaaaaact tgacttagat tacaaattaa tacttgatat cttattttat
    41581 agtataagta catgtggata taatgaaaca gaaaaaagtt ggttttcatc tatatattca
    41641 aatgtccttg actctaaaaa acaaaataaa atatatacaa aacttgaaaa acttaaattt
    41701 aatggactta caagaaaact tttaagagtt ttactaaatg agtttgacca taaagaaata
    41761 tttaactatt gtttagaaat atactataaa cttagaaact tattgactat agaacaatgt
    41821 aaaaaacaaa gagttgaatt tactaaaaac tattacagac aaaatgaata ctgccctgct
    41881 ttaaatgccc ctattttaaa ctattggctt gataaagcac attcgtttat ttatgatgat
    41941 atttctttta ctaagttttt ctacactacg ttttcaaatt atcagttgct agaatataag
    42001 tttcctttct atactagcat aaatgtgttt gctcgtgcac atgaattagg attaatgact
    42061 gaagagcaag ttaatagaga aatgataagt ggatacaacg catcaaatat aataagaaca
    42121 ttaacaaatc ctagatgggc tggcgacaca ttagaaaagt acccttggct taaagataat
    42181 ttaaaaactg ttacaaaccg aattatagaa attgaatcac gtagaggtga tatgccaaca
    42241 gaagtgtcta atctagctaa taatatacaa tactttgaag gtataaatta ttttgtaaat
    42301 attttggtcg ctcttggtaa agaaactttc cagagaggat attcatatgg tagcaattat
    42361 tcaaaaaaag atgttttaag ttctctactt aaaaattgct atcctactaa agaagatgac
    42421 tcaaaaaaat taaaaagttt acttaaaaat accgacatta ctgagttaag attgatagaa
    42481 tctgctatgt atgcccctca atgggtaaac ataattgaaa aatatttaaa ctggaagggt
    42541 ctgaaaagta caatttggta cttccatgca catataaatg aaggcttctc tgcacaaaaa
    42601 gaatcagagg ttgctctatt ctcccctatt tcaccacgta gtttcattga tggtgcattt
    42661 gatgtagatt ggtttttaga ttcatataaa actattgggg aagaaagatt caatatactt
    42721 tacaagtcag ctaaatatat aactagtggt ggtaactctc atagacgtgc acaactctat
    42781 gctgatgcta cacttggtaa gcttgataaa gacatgttga tgaaagaaat tgaaacaaat
    42841 agaaatcaag ataaattgag aagtttctct ttacttccta tgaaaaaagg agacctaaaa
    42901 gaagcaactt tccgttatga atttattcaa aagtttgcta aagaaagcaa gcaatttggt
    42961 gcacaacgtc gtgaaagtga atcaaaagct tgtactatag cgctagaaaa tctagctaga
    43021 agtgctggat ttggagatgt aaatcgtatg atgtgggctc ttgagactga aaaactaaat
    43081 gaagttgctc atctatttaa accaaaagat attgatggta cttcaatttg gatagatgtt
    43141 gatgaaaacg gaaaagcatc tactaaagtt caaaaaggca acaagtcttt aaagagtata
    43201 ccaaaagcta ttgctaaaca tccatatgta cttgaactta aatctgtcca aaaagattta
    43261 aagagtcagt attctcgtgc aaaacagagt ttagaacaag ctatgaaaaa tgaaatagta
    43321 tttacagtta aagaaattgc caatatctat aaaaatccag ttatctgccc tttgattaaa
    43381 aatctagtat gggtatgtga tgataatatt ggatatgttt ataatgataa gtctaagttc
    43441 tttatttgca atataaaagg tgaaacttat aagttgacgg caaaaaataa attaaaactt
    43501 gcacatccag ttcatttgtt tgaatctggt aagtggagtg agttccaaca tgatttattt
    43561 agtaagcaaa ttgttcaacc atttaagcaa gtattccgtg agtattatag aataaatagt
    43621 gatgaattat ctgaaaaaac tatatctcgc agatatgctg gtcaccaaat tcagccaaag
    43681 aaaactgtag cacttcttag aaatcagctt tggactgtcg attatgaaga gggattacag
    43741 aaagtatttt ataaagaaaa tatagtggca aagatgtttg cacttgcaga ttggttttca
    43801 ccagcagata tagagcctcc tactattgaa acagtagaat tttttgatag aaaaacttat
    43861 aaaagtttag aattagcttc tatacctcca attctattct cagaagttat gcgtgatata
    43921 gaccttgttg taagtgtcgc tcatgctggt ggtgttgacc ctgaaactag ccattctact
    43981 gtagagatgc gtatagcaat tgctaaagaa cttttattat tattaaaaat taacaatgtc
    44041 aattttgaag gctctcatgc tcttatagca ggtaatttag gtgagtattc tgtgcatatg
    44101 ggttctggta ttgtacataa aaaagggatg ggtgctttga atatacttcc tatacattct
    44161 caaagtagag gtaagatatt ccttccattc gctgatgaag accctaaaac tgctgagatt
    44221 atgtctaaga tactattact tgctgaagat agtaagatta aagaccctag tatcttaagt
    44281 aatttataag tataaaaact atctctaaaa tatattggac tttcttaaaa aaattagaaa
    44341 gtccaatata tactatactg taatttatat agattaaatc ataattacaa ataattttta
    44401 tataaacttc tcttatacat tatctttacg gtacttatta tatcacacaa aaagtatttt
    44461 tcttctatct tcgctagaat cttttaaccc tataaaatct atataaaaaa tctttttcta
    44521 gagatttgtt taatatggca ttttcttgaa aatttataaa ccttatctct aatgaagatt
    44581 tatttatagg tataacctta tgtataaatc ttagatttac tatgtattgt ctatgggtca
    44641 taaagaaatt aggaggtagg cgttttttta aatcatcaaa gttctcataa aactttatta
    44701 tatcttcatc tttagtgtgt agattaattg ctcttgaatt tttctcaaac ataactatat
    44761 catcaaaatt aatcatatgt atctgcttcc ttattttata tacaaaaagg gtgtcttcta
    44821 taaaattatt attagcaatc ttatgggagt taatatggtc tattgctatg ttaattgaat
    44881 caagtatttt ttctttttta aatggtttaa ctacaaaatc tataggatgc actttaaagc
    44941 tttcaagact ataatcagcg aaacccgtca caaatactat atgtatatcc ttatctatag
    45001 aaagaatttc ttcagcagaa tcaatcccat ttttatatgg catatcaatg tcaataatca
    45061 ataaatctat tttctcattt tttataaatt caattgcctc tataccatcc ttaacttttt
    45121 taacataatc tacatatcca gactctttag ctatatttgc tatatcatct cttacaatct
    45181 tatcatcatc acaaatcaaa acttttatca tagacttttc tcctcctaac taataatatt
    45241 ttgggattaa gattacaaat tcagttaaaa aatcatcact atttacaagt atactataat
    45301 catacttatc taatatattt ttaactaaat gtagcccata tcccctatct tgtccttctt
    45361 tagtactaaa tccattccaa aatatacttt caacattatg tattttagga ccattatttt
    45421 ttatagctat aatatactga aattcatctt catatgcatg taatcttata tacttctttt
    45481 ctttttttat agtagacaaa gactctattg cattatctat tatgtttaat agtactgaac
    45541 tcatatcatt aggttcaatc tctaaattat ccataaaatc ttgaatgtag acatcaaaag
    45601 atatatcctt ttcaagacat tcctcgtatt ttaatgaagc tatagcatct aaataagata
    45661 cttttccata ccttgaattt tttgatttaa agctatttga tatattcatt atatattctt
    45721 tggcatcttc atctagccca gtatttaata gagaatacac tacttgcaaa tgtttcaaat
    45781 agtcatgttt ttgacttctt aatttgataa ttatattatc cttatgattt attttactat
    45841 ctagtttttg taatttatat tcctgttcat tattattatt tatcaatctt ataactttaa
    45901 agcctgtata aaaacttaaa ataattaaaa ccaaaaaaag tataatattt aatactcctt
    45961 caagtaaaaa actatatcct aaccttaatt gtatatattt agtgccctcc ataaataaaa
    46021 tcaatgcaaa acacgatata aaaaatgtta atatgcctct ttttaatatg tctaattctt
    46081 tgtaaaattt atacttattc attataagat tgctaaatat aaacaaagtt agtattacta
    46141 taggaaatac aaaattgtta tccatgattc ccctcccctc tacaatttat atagggatta
    46201 tatcatttta aaaaaattat gtaaaaagtt aaaatttaca tatatattaa ttaaacttgt
    46261 aaaaatacag atgaatttgt acaattaact acttaaaact tgaaaatact tactttgcta
    46321 tatattttag ataaatatat aattgattta gttaagaaag gaagtggtaa atatgaaaga
    46381 taatttgaaa atagtactaa ggtatataaa aagttataaa gctaggtctt tagcaattgt
    46441 tgtgagtata attcttgcta catcactcgt tgttggtatt ggtaccctct caagaagtgc
    46501 tcagcaagca gatgtagata gattgaaaag agagcttgga actgaccatg tatactttaa
    46561 aaatataaag aaaaatcaat taaagtccat aaaatctagc aaagatatta aaaatctggg
    46621 tataacatct tactatgcgt ctacagatgt taatgaaaaa ttacctataa acatactata
    46681 tgcaaataaa aattatttga ctagtcattc taaacttata aaaggtcgat ttccaaaagc
    46741 tagcaatgag gtggtagtcg agaaatggat tttaaatagt atgggcttaa aaccagagat
    46801 aaatcaaaac cttactttta aattatatca aaaagaaaat ccagaaactt ttaaagttgt
    46861 tggaatcctt gaagatagat ttatagagaa aaaaaaggga caatgtgaaa ttttttctaa
    46921 tctcaatgaa tcagaactga atgattttac aacttatatt gaatttaatg aaggcagtga
    46981 tataaataca aacatagaca atataataaa aaatgctatg ttagatgaga aaagtatagg
    47041 aagaaactca atgcttatag aatctactat gaaaaacggt actttagata attcaagtaa
    47101 atacgttgca attgctatga gtctatttgc tggtattgta atatatagta tctatgttat
    47161 atcaatatac caaagagtat aggaatatgg cgtcttaaga gctattggtt ccaccaactt
    47221 taggattttc aaacttatgc tttatgaact tttcatactt actctaatag caatgcctat
    47281 aggtatatgt ataggtatgg gaggtgcaca aatatttaat aggtctgtag gaaatattca
    47341 atttgaagga aatataaatg ttacaccttt tgttattcca gataaaataa ttctcttatc
    47401 aattgcatgt acaatactaa taatactaat tataagtttc tttacatact taaaaataag
    47461 aagaatttct cctattgatg ctatcagaaa aacttttgga gctgataaaa atgtaaccaa
    47521 agttaattat ttaatatcaa aattaacttt aaatatgtca gtaacaaaat ctatatctgc
    47581 gaaaaacatc tttagaaata aaaaggggtt cattataatt gtattatcta tgagtatagg
    47641 aggtataatc gtcataaaag acaattataa atattctttt ttagatgtgc aaaacaaatc
    47701 tactcaagaa caaacatata tgaatgcaga ttttattcta tcaaatgttt atctcaatga
    47761 aattggtaac aatacttctt ttaaagatat aaaagggttt aatgataatc agatagataa
    47821 aataaaaaat atagatggaa ttgacaaagt caaaactgct agtatcttaa acacaagaat
    47881 agaggtaaat aaaataaata gattagatta ctatgaagct ataaattcaa ctccttatta
    47941 taagctaaat cctctgttca taaaaaataa gactactggc aaatatacac taaagcaaaa
    48001 gctaaaaggt tataatgatg agatgataaa gtcattagaa aaatacttag taagtggaaa
    48061 tataaacttg aaaaaaatga aaaaagaaaa tttagctatt gtatatgtac cagaaatttc
    48121 taaaacacag aaatacacaa agacttttac tcctggtgaa ggaacaccag ctgtagatat
    48181 aaaagtaggt gatactataa aagtcaaata tccaaagggt gggatggata caggtcttta
    48241 tgaaagtcta aaagataatt atgaatactt agaatatgaa tttaaagttg gagctattat
    48301 aagttatcct tttgcagata atggtatgta ctctggagat gatggtgtag atgtaataac
    48361 gagtagcgaa tacctaaaaa aattaactgg tgtagataag tataacttag tttatgcaga
    48421 ccttaatgaa aatgcaaaca agaaaaaaat aaataacctt ttaggtaaaa ttggaagtga
    48481 agttcctgga actacaacag tagatatgat ggtagaaaaa gaaaattctg ataagatgac
    48541 atctagaact atgatttata gttatggtat agtagcaata atgttcataa tcagtgtact
    48601 taacataata aacaacataa gttataactt aacttcaaga actagtgaat ttggaatgtt
    48661 aagagctatt ggtatctctg agagaggctt taaaaatatg atactttatg aaggaatatt
    48721 atatggtgta ctctcaagta taattactat cgtgtcagga attataatac aatttaaaat
    48781 gtattatatg cagggctttg tatcatatgg attgggattt tcaatagatt acaaaatata
    48841 tatactagtt gttgtagcaa atataattgt aggtattcta gctacatata ttccatcaag
    48901 aaagataaat aaaattagta tagttgaagc tataaacatt acagaatagg aggaataatt
    48961 atgcctatat tggaaactat taacttaggt aagatttatg gaaaaaagga aacatctgta
    49021 catgcactaa agaatgcaaa tttaaaaata aataaaggcg aatttgttgc tataattgga
    49081 ccaagtggaa gtgggaaaag tacatttcta catttagtag gtggtcttga aagaccaagt
    49141 aatggtacta taaaggtaga tggtaaagat atatgttgtc tttcagataa agaattagca
    49201 agatatagaa gacaaaaggt aggatttgtc tttcaacagt ataacttaat tcctgtatta
    49261 aatgtaaagg aaaatataga attaccacta aagttagaca ataaaaaaat agataaagaa
    49321 tatattgatg accttataaa tttattgggt cttaatgaaa ggaaaaatca tcttcccaac
    49381 caattatctg gtggtcaaca gcaaagagtt gctattgcac gtgctttatc tgcaaaacca
    49441 agtataatcc tagcagatga accaacagga aatttggaca gtaaaactac agaagaagtc
    49501 atggatttac taaaaagttc tataaaaaaa tataaccaaa cccttataat aataacacat
    49561 aatgaaaata ttgctagaaa agcagataga attatatcta taatagatgg agagcttaaa
    49621 ttaacactct aaaactattt atagaaaaga ggatttaaat gttattgatt ttaggtaatt
    49681 taaatttaaa cttggatttt gactacagaa taataagaga agaaaatgat gatgtagata
    49741 tatttataga tattaattat cgtagcttag atatagatac tgatggttct aacttattta
    49801 attctagaat acaatttcca tttgtaagag cattgattct aaggctaaat aagaacaatc
    49861 aatgtatgac aattcatttg ttaagagata tagatttatt ctctgctttt gccaactttg
    49921 aagtggatta tactgactct ataataaata taaaaaatca aaatgagaaa gtaattttaa
    49981 ataaatctat caaaaaatag taagggggcc ttaataggcc ccaatttttt tcttttctaa
    50041 acttattata tcatgataaa taaattagtc aatagaattt tctgaatatt tttataattt
    50101 tttcataaag ttttttatat attatgcttt aaaattatat gtaataaaaa aattttccga
    50161 ctaattttaa agttaattga ttaaatacac atattcgaaa ttaccattaa attactgact
    50221 ttttatcatt tccatttaga atttaaatta taatctatta tttatagtta cttctacgtg
    50281 tctctaataa aactctattt atattcaaat ctaataattt atatacaaac ccaatagaac
    50341 ctatgttgca caatagatat tatttaccat aataaacttt tagatatatc tcttttaatt
    50401 cactcatcaa tgggtatcta ggattagcac ctgtacattg gtcatcaaaa gcttgttcaa
    50461 ccatagcatc caatctttct aagaatttag attcttctac tccagcctct tttatagtct
    50521 taggaagacc tacttttgcc tttagttcat ctattgcttt tataagatta tctacttttt
    50581 cttggtctgt acttccttta atacctgcaa aagaagcaaa ttcagcatat ctttgtatgc
    50641 aatcaggata tctgtattgt gagaatgcac ccattttatt tggagcttct gcacaattga
    50701 acttgattac ttcatttatt aataatgcat ttgcaacacc gtgttgaaca tggtggaatg
    50761 cacctaattt atgtgctaaa gagtgacata ttcctaagaa ggcatttgca aaagacattc
    50821 ctgccatagt agatgccatt gccatttttt ctcttgcttc tttatcattc tttccatttt
    50881 tatatgcacg aggtaaatat tcaaatatta ttttacctgc ttgtagtgct agaccgtcag
    50941 ctggttcagt tcttaacata gatacatatg cttctaatgc atgtgttaaa gcatcaacac
    51001 ctgatgctgc tgttaatctt ggtggcatct ccatcatcat atcagcatca attatagcca
    51061 tgtttggcat taattcataa tctgctaatg ggtactttac acctgaatct tggtcagtta
    51121 taactgcaaa tggagttact tctgaaccag ttcctgctga agttgggata gcagcaaaat
    51181 acgctttttc acccatctta ggaaatacat atactctttt tcttatatcc ataaatctca
    51241 tagctaagtc ttggaaatct acttctggat gctcatacat tacccacatt atcttaccag
    51301 catccattgc acttcctcca cctacagcta ttattaaatc tggttggaaa tcagccatag
    51361 ctttagctcc ttctcttgca caagctaatg ttgggtctgg agctacatca aagaatgtag
    51421 tatgcttaat tcccatctca tctaataaat cagttaattt cttagtatat ccattattat
    51481 ataggaaaga atctgttact ataaatgctt tctttttatc cataacatcc ttaaattctc
    51541 ttgctgctac acctaaacat ccttttttga aataaacctt ttctggtgct ctgaaccaaa
    51601 gcatattctc tctcctttca gctactgtct tgatattaag caaatgtttt actccaacgt
    51661 tttcagatac tgagtttcca ccccaagaac cacaaccaag agtaagagac ggtgccaatt
    51721 taaagttata taaatcacct ataccacctt gtgatgatgg agtattaact aatactctac
    51781 aagtcttcat agcatttaca aacttttcta ctctctctgg atgatttata gagtctgcat
    51841 ataatgaaga tgtatgacca tagcctccat ctgcaactaa tttttctgct ttagcaactg
    51901 catcttcaaa acttttagac ttgtacattg caagtacagg agaaagtttt tcatgtgcaa
    51961 aaggttcatt taattcaaca gattctactt caccaattat tattctagct gtttttggaa
    52021 cttctacatc cgccattttg gctattacat gtgctggttg tccaactatt ctggcattta
    52081 atgaaccatc aattattact acttttccta ctttttcagt ttcttcttta tttaggaaat
    52141 gtgctccacg atatttaaat tcttttttaa cttcttcata tattttttct ggtactatta
    52201 cagcttgctc ggaagcacat atcataccat tatcgaatga ttttgatact aagattgaac
    52261 ttacagccat ttttatatct gcactctcat ctattattgc aggtacattt ccaggcccaa
    52321 ctccgacagc tggtttacct gaagagtatg cagatttaac cattccaggt ccaccagtag
    52381 ctaatataag gtctgcctct gccatgacaa cattagttaa ttcaatcgaa ggaacatcta
    52441 cccaacctat aagtcctttt ggtgctcctg ccttaactgc tgcatcatgt acaactctag
    52501 ctgcttctat agtacagttt ttagctcttg gatgaggaga gaaaattata ccatttctag
    52561 tttttagtgc tattagggat ttgaatattg cagttgatgt aggattagtt gttggtataa
    52621 ctgctgataa aacacctact ggctcagcaa ttcttgtaaa accaaatgct tcatcttttt
    52681 ctatgacacc acaagtttta gtatctttat aagcattata gatatattct gaggcaaaat
    52741 ggttttttat aaccttatct tcaacaatac ccataccagt ctcttccact gccatttttg
    52801 ctagaggtat tctttgttga ttagctgcag ttgaagcagc taagaaaatc ttatctacct
    52861 gttcttgcgt aaatgttgca aattcttttt gtgccaatct aatttcttct aatctcattc
    52921 ttaatgtttc aacactgttg acaatttcag gactacatac ttcaacattt gatttttcta
    52981 ctaccttttc ttttttctcc atactaaaaa cctccctaaa ataattatgc atctctaatt
    53041 tttataaatc ttaaatgcat catctatatt tttaacttat acttaaaata attttataga
    53101 tttcatctct ataccttaga ttttatttat tttactctct taggtcaaga agtttttttc
    53161 tgaatatttt gtcaatttat tttttaacaa atatattatt ttttttactt ttactaaaat
    53221 tttttatcta aatgagatat atttcatttg aataa
//
LOCUS       DAGSWI010000009        48834 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.009, whole genome shotgun sequence.
ACCESSION   DAGSWI010000009 DAGSWI010000000
VERSION     DAGSWI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.009"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..1180
                     /locus_tag="MJF80_000470"
     CDS             <1..1180
                     /locus_tag="MJF80_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBZ0279149.1"
                     /translation="NCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHEKAGRDD
                     KYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFATSVVITP
                     EVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSSYLYGLP
                     HELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAAVDGGKC
                     VDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVYGMTGIS
                     SDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVGENGKAI
                     RADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARDTLRLVK
                     "
     gene            1273..1797
                     /locus_tag="MJF80_000471"
     CDS             1273..1797
                     /locus_tag="MJF80_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBZ0279150.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1822..1998
                     /gene="rpmF"
                     /locus_tag="MJF80_000472"
     CDS             1822..1998
                     /gene="rpmF"
                     /locus_tag="MJF80_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBZ0279151.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2205..2762
                     /gene="fapR"
                     /locus_tag="MJF80_000473"
     CDS             2205..2762
                     /gene="fapR"
                     /locus_tag="MJF80_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBZ0279152.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2773..3795
                     /gene="plsX"
                     /locus_tag="MJF80_000474"
     CDS             2773..3795
                     /gene="plsX"
                     /locus_tag="MJF80_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBZ0279153.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3821..4807
                     /locus_tag="MJF80_000475"
     CDS             3821..4807
                     /locus_tag="MJF80_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBZ0279154.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            4916..5845
                     /gene="fabK"
                     /locus_tag="MJF80_000476"
     CDS             4916..5845
                     /gene="fabK"
                     /locus_tag="MJF80_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBZ0279155.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5861..6811
                     /gene="fabD"
                     /locus_tag="MJF80_000477"
     CDS             5861..6811
                     /gene="fabD"
                     /locus_tag="MJF80_000477"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBZ0279156.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDVLNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAILGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6811..7560
                     /gene="fabG"
                     /locus_tag="MJF80_000478"
     CDS             6811..7560
                     /gene="fabG"
                     /locus_tag="MJF80_000478"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBZ0279157.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILINNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7614..7838
                     /gene="acpP"
                     /locus_tag="MJF80_000479"
     CDS             7614..7838
                     /gene="acpP"
                     /locus_tag="MJF80_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBZ0279158.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7867..9105
                     /gene="fabF"
                     /locus_tag="MJF80_000480"
     CDS             7867..9105
                     /gene="fabF"
                     /locus_tag="MJF80_000480"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBZ0279159.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9331..11049
                     /locus_tag="MJF80_000481"
     CDS             9331..11049
                     /locus_tag="MJF80_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBZ0279160.1"
                     /translation="MNFRLPKKRLPKKIIFICIVLISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11080..13080
                     /locus_tag="MJF80_000482"
     CDS             11080..13080
                     /locus_tag="MJF80_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0279161.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13431..13676
                     /locus_tag="MJF80_000483"
     CDS             13431..13676
                     /locus_tag="MJF80_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBZ0279162.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13711..14436
                     /locus_tag="MJF80_000484"
     CDS             13711..14436
                     /locus_tag="MJF80_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBZ0279163.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14458..15771
                     /locus_tag="MJF80_000485"
     CDS             14458..15771
                     /locus_tag="MJF80_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBZ0279164.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            15977..16516
                     /locus_tag="MJF80_000486"
     CDS             15977..16516
                     /locus_tag="MJF80_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0279165.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16605..18590
                     /locus_tag="MJF80_000487"
     CDS             16605..18590
                     /locus_tag="MJF80_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBZ0279166.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18814..19782
                     /gene="spoIIIAA"
                     /locus_tag="MJF80_000488"
     CDS             18814..19782
                     /gene="spoIIIAA"
                     /locus_tag="MJF80_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBZ0279167.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19772..20293
                     /locus_tag="MJF80_000489"
     CDS             19772..20293
                     /locus_tag="MJF80_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBZ0279168.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20310..20507
                     /gene="spoIIIAC"
                     /locus_tag="MJF80_000490"
     CDS             20310..20507
                     /gene="spoIIIAC"
                     /locus_tag="MJF80_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBZ0279169.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20523..20909
                     /gene="spoIIIAD"
                     /locus_tag="MJF80_000491"
     CDS             20523..20909
                     /gene="spoIIIAD"
                     /locus_tag="MJF80_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBZ0279170.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            20994..22199
                     /gene="spoIIIAE"
                     /locus_tag="MJF80_000492"
     CDS             20994..22199
                     /gene="spoIIIAE"
                     /locus_tag="MJF80_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBZ0279171.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22259..22876
                     /locus_tag="MJF80_000493"
     CDS             22259..22876
                     /locus_tag="MJF80_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBZ0279172.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22888..23442
                     /locus_tag="MJF80_000494"
     CDS             22888..23442
                     /locus_tag="MJF80_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBZ0279173.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23469..24158
                     /locus_tag="MJF80_000495"
     CDS             23469..24158
                     /locus_tag="MJF80_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBZ0279174.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNNDESDSSKTEDSSNIDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24268..24630
                     /locus_tag="MJF80_000496"
     CDS             24268..24630
                     /locus_tag="MJF80_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBZ0279175.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24715..25221
                     /gene="nusB"
                     /locus_tag="MJF80_000497"
     CDS             24715..25221
                     /gene="nusB"
                     /locus_tag="MJF80_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBZ0279176.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25205..26275
                     /locus_tag="MJF80_000498"
     CDS             25205..26275
                     /locus_tag="MJF80_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBZ0279177.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGYNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSVKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26272..27477
                     /locus_tag="MJF80_000499"
     CDS             26272..27477
                     /locus_tag="MJF80_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBZ0279178.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27479..27694
                     /gene="xseB"
                     /locus_tag="MJF80_000500"
     CDS             27479..27694
                     /gene="xseB"
                     /locus_tag="MJF80_000500"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBZ0279179.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27697..28584
                     /locus_tag="MJF80_000501"
     CDS             27697..28584
                     /locus_tag="MJF80_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBZ0279180.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28614..29051
                     /locus_tag="MJF80_000502"
     CDS             28614..29051
                     /locus_tag="MJF80_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBZ0279181.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29085..30950
                     /locus_tag="MJF80_000503"
     CDS             29085..30950
                     /locus_tag="MJF80_000503"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBZ0279182.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDEGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31083..31895
                     /locus_tag="MJF80_000504"
     CDS             31083..31895
                     /locus_tag="MJF80_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HBZ0279183.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            31915..33594
                     /gene="recN"
                     /locus_tag="MJF80_000505"
     CDS             31915..33594
                     /gene="recN"
                     /locus_tag="MJF80_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBZ0279184.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33808..34674
                     /locus_tag="MJF80_000506"
     CDS             33808..34674
                     /locus_tag="MJF80_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279185.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVTVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDGIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34767..35258)
                     /locus_tag="MJF80_000507"
     CDS             complement(34767..35258)
                     /locus_tag="MJF80_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0279186.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35319..35759)
                     /locus_tag="MJF80_000508"
     CDS             complement(35319..35759)
                     /locus_tag="MJF80_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0279187.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            35920..36978
                     /locus_tag="MJF80_000509"
     CDS             35920..36978
                     /locus_tag="MJF80_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBZ0279188.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37120..37944
                     /gene="spo0A"
                     /locus_tag="MJF80_000510"
     CDS             37120..37944
                     /gene="spo0A"
                     /locus_tag="MJF80_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBZ0279189.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38357..39478
                     /locus_tag="MJF80_000511"
     CDS             38357..39478
                     /locus_tag="MJF80_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279190.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39496..40371
                     /locus_tag="MJF80_000512"
     CDS             39496..40371
                     /locus_tag="MJF80_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBZ0279191.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40395..41087
                     /locus_tag="MJF80_000513"
     CDS             40395..41087
                     /locus_tag="MJF80_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBZ0279192.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41095..41919
                     /locus_tag="MJF80_000514"
     CDS             41095..41919
                     /locus_tag="MJF80_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBZ0279193.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            41924..42991
                     /locus_tag="MJF80_000515"
     CDS             41924..42991
                     /locus_tag="MJF80_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBZ0279194.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            42993..43529
                     /locus_tag="MJF80_000516"
     CDS             42993..43529
                     /locus_tag="MJF80_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBZ0279195.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43597..44202
                     /locus_tag="MJF80_000517"
     CDS             43597..44202
                     /locus_tag="MJF80_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBZ0279196.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44214..45128
                     /locus_tag="MJF80_000518"
     CDS             44214..45128
                     /locus_tag="MJF80_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBZ0279197.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45170..46342
                     /locus_tag="MJF80_000519"
     CDS             45170..46342
                     /locus_tag="MJF80_000519"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBZ0279198.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46389..47201
                     /locus_tag="MJF80_000520"
     CDS             46389..47201
                     /locus_tag="MJF80_000520"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBZ0279199.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47405..48730
                     /locus_tag="MJF80_000521"
     CDS             47405..48730
                     /locus_tag="MJF80_000521"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBZ0279200.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT        19746 a         4928 c         9178 g        14982 t
ORIGIN      
        1 aaactgtggg agttcttcat taaaatatca attaatagat atgaataatg aagaagtttt
       61 atgtatagga ttggttgaaa gaataggaat agaaggttct atattaaaac atgaaaaagc
      121 aggtagagat gataaatacg ttgttgaaca accaatgaag gaccacaaag atgcaatagc
      181 attagtttta gaagctgttg ctcatccaga atttggtgca gttaaagaaa tgaaagaaat
      241 agatgcagta ggacatagag ttgtacatgc tggagaaaaa tttgcaactt cagtagtaat
      301 aactccagaa gtagaagaag ctttaaaaga atgtatagat ttagctccac ttcataatcc
      361 agcaaacata atgggaatag atgcttgtaa agctatactt ccagatgtac caatggtagg
      421 tgtatttgat actgctttcc atcaaacaat gcctaaatct tcatacttat atggtttacc
      481 tcatgagcta tatactaaat atggggtaag aagatatgga ttccacggaa cttctcataa
      541 ttatgtatct cagagagcag cagagatact aggaaaagat ataaaagatt taaaaatagt
      601 aacttgtcac ttaggaaatg gtgcttctat agctgctgtt gatggtggaa aatgtgtaga
      661 tacatctatg ggattcactc cattagaagg attaataatg ggaactagat gtggagatat
      721 agacccagct atattaccat tcttaatgag aaaagaaggt ttagatgctg atggattgga
      781 taaattaatg aataaagaat ctggagtata tggaatgact ggaatttcta gtgacttcag
      841 agatatagaa gatgcagcta aaaatggaga cgaaagagct caagctacat tagaagcgta
      901 tgttaaaaaa gtacaaaaat atataggcgc ttatgctgct gaaatgaatg gattggatgt
      961 tgtagtattt actgcaggcg ttggtgaaaa tggaaaggct ataagagcag atattgcttc
     1021 taacatggag tttttaggaa tgaaattaga taaagaagct aatgatgtta gaggaaaaga
     1081 aacagtaata tctactgctg attctaaagt aaaaatgctt ttaataccaa ctaatgaaga
     1141 attaatgata gctagagata cattaagatt ggtaaaataa ttaaattaaa cctatgccta
     1201 tcaagtaaaa atacttgaca aacaaatgct agatttgtat aataaatttg gtgatatgtt
     1261 gaggtgaact taatgaaagt tagcatagaa aaaataaata gaaaagaaac tgacaaaata
     1321 gacttgaatt tttgtgaaaa aattgatact attagttatt gtgatgagat ttacaaatta
     1381 gtatcacctg taaatttaaa agggaaagtg tcaaagacta ataaaggttt gtatctagat
     1441 atagatgtca attttacaat tgttgacaat tgttcaagat gtcttaaaga agtagaaata
     1501 ccactagagt attctataca aggtttttta gtaaaagaag aagattatga tgaagatgag
     1561 tttgaggaat ttgatccttt tatattcgat ggtgaagaaa tcgaccttat tgatataata
     1621 gaacagacat tagattttaa tgtacctcat aaagttcttt gtagtgaaaa ctgtaaaggt
     1681 ctttgtcaag tatgcggggc aaacttaaat gaagaagagt gttcttgcag tgaaattaca
     1741 aacgatgagg aatacataga tcctcgtttt gctaaattaa aagatttatt taattaaatt
     1801 ttgactaagg aggtggcgta aatggcagta ccaaagcgta aaacgtctaa atcaaacaca
     1861 aaaatgagaa gagcagctaa ctcaaagatg gaagcaacag gatttgtaag ttgcccacaa
     1921 tgtcatgagc caaaattacc acatagagtg tgtccagact gtggatatta taaaggtaaa
     1981 gaagttgtgt ctaagtaata gcttctaaaa agcatgtaga gtatactcta catgtttttt
     2041 ttgtacatat aaaaaaataa gatgatttac tagagaaaat aagtggattt tatgcatatt
     2101 aaaaaataga aaaataataa tataagtatt gaaaattttt gtgtgttact atatactact
     2161 attagtagct ggtactaatt acagctataa aatttggagg gaacatgaaa aagaaaagta
     2221 aagcccaaag acaaaaagag cttatagata tgctaaagac agatcctttt tatactgatg
     2281 aagaattatc aagtctgttt gatgtaagta tacagaccat aagattagat agaatgagtc
     2341 taaacatacc agagctaaga gaaagagtca aatcaatagc agaaactcag agttcaaaag
     2401 taaagacttt aggagtaaag gaaattacag gagaaatcat tgatttatct gtagggcgat
     2461 taggtatatc tatgttagaa gttacacagg acatgattta ctctaagact aatactttaa
     2521 aagatacgta tattttttct ctagcagatt cattggcaat ggctataata gatgcaccta
     2581 aggttattat gagagttgca aatgtaaaaa gttttaagtt gatagaacaa caagataggc
     2641 ttatagcaaa agcagaagtg tatagaaata tagataaaaa gcattatgta aaagttgtta
     2701 tcaacaataa agcccaagag caaatattta gaggaaagtt tatctttgaa gaattagatt
     2761 aggagggaaa ttatgaaaat agtaattgat ggaatgggtg gagataatgc acctaaatct
     2821 aatgtagaag gcgcagtaaa tgctataaaa gaatatcagg ttgatttaat aatcacagga
     2881 gataaagact tacttgaaaa ggaattttca aattatgaat ttgatagaaa taaattagag
     2941 atagttcata ctactgagat tatagaaaac gaagataaac cagtaaaggc tataagaagt
     3001 aaaaaagatt catctatggt agtagcatta aatcttgtaa aagaaggaaa ggcagatgct
     3061 ataatatcag ctggaaatac aggagcctta cttgctggtg gattatttgt tgttggtaga
     3121 ataaaaggaa tcgatagacc ttgcttatgt tcagcaatac caaatgtaaa gcgaggaatg
     3181 acacttatag cagactgtgg cgcaaatgca gattgtaaac ctaagaatct agttgaattt
     3241 gcagctatga gtaatattta ttcaagaaaa gttttaggtc ttgaaaatcc taaggtagca
     3301 ttagcaaatg ttggactaga agaaggcaaa ggcaatgatt tagtcaaaag gtcatatgaa
     3361 gaaattaaaa aattagattt aaattttata gggaatgtag aagctagaga agttataaat
     3421 gcatacacag atataatcat atgtgatgga tttacaggta atatattact taaatctgct
     3481 gaaggtgtag cactttcagt tatgagtctt ataaaggaaa cttttatggc cagtacaaaa
     3541 agtaaaatag gagcattact tataaaggat gatttaagaa aattaaaaag ttttatagat
     3601 tattctgagt atgggggagc tccactttta ggtctaaatg gaggagttat aaaagctcat
     3661 ggtagttctg atgctaaagc tataaaaaat gctataaatc aaggaataaa attttcaaag
     3721 ggaaaagtcg ttgaagatat taaccaattt ataagcaaat acaatgaaga aaataaaaat
     3781 aacgaagatg aataaactcg ttttggaggt ttaatatata atgaatacaa aggctggaat
     3841 attaggagta ggaagttatt tacctgagca atcatatgat aattttcact tcgaaaaaat
     3901 aatggataca tcagatgagt ggataagtac tagaactggt attaaggaaa gaagatttgc
     3961 caaagagtct gaagcaactt ctgacttagc atctaaagct gccttaaaag ctatagaatg
     4021 tgcaaagtta aacgtagagg atatagagct gattatatta gctacaatta ctcctgatat
     4081 gtctttgcct tcaactgcat gtatagtaca agatgcaata ggagcagtga atgctactgc
     4141 atttgatata tcagcagctt gttctggatt tgtatatgga gtaactattg caaagcaatt
     4201 tgtggaaaca ggttgctata aaaatgtgct tgtaatagga gcagaaactt gttctaagtt
     4261 tttaaattat gatgatagaa caacagcagt attgtttgga gatggagctg gagcagctgt
     4321 tattggccct gtaaatgagg gtggaatact gtctacacat atgggctctg atggtaaagg
     4381 aaaagattgt ttaaaagtac ctgctggagg ttcaagactt aaggcaagta aagagactgt
     4441 agaagctaat ctacatacaa tagaaatggc aggtagtgat gtgtttaaat ttgcagttag
     4501 aaagatggca gaaacttctt taagagctct tgaaaaagca aatctaaata caactgatat
     4561 agactactta gtgccacatc aagctaatat aagaatcata caagcttctt ctaagagatt
     4621 agagcttgat atgaaaaaag tttatgtaaa tatagacaaa tatggaaata tgtctgctgc
     4681 atctatacca gtagcattag atgaagctta tagagaaggt aaaattaaaa agggtgataa
     4741 tgtagtttta gttggctttg gtggaggtct aacttggggg gcttcagttg tcaagtggac
     4801 tttatagtat aaaatatcta aaatgattta attaacaaaa ttaaacttat acacaaaata
     4861 aagttgatta ataataaaaa gtttactttt atgatttcaa gtgaaggaga attgtatgaa
     4921 taaaatttgc aaaatattaa atataaaata cccagttatc caagggggaa tggcatgggt
     4981 agctactgca tcattagcaa gtgctgtatc taatgcagga ggacttggca taatagcagc
     5041 aggaaacgca ccaaaagaag ctataaagaa agaaattgtt gagtgtaaaa aattaacaga
     5101 taaacctttt ggagtaaatg taatgcttat gtcgccattt gttgatgata taattgattt
     5161 gattatagaa gaaaaagttc aagttattac tactggtgct ggaaatcctg caaagtatat
     5221 ggatagatta aaggaagctg gaacaaaggt tattcctgta gtacctacaa tagctttggc
     5281 acaaagaatg gaaaagctag gagctacagc agtaatagca gaaggtactg aaggtggagg
     5341 acatatagga gaacttacta ctatggtctt agttccacaa gttgctgatg ctgtaaacat
     5401 acctgtaata gctgctggag gaattgtaga tggtagagga attgcagcat catttgcatt
     5461 aggtgccagt gcagttcaag taggaactag atttatttgc agtgaagagt gttctgtcca
     5521 ttcaaactat aaaaacttag tactaaaagc aaaagataga gatgcaattg taacaggaag
     5581 aagtactggt catccagtaa gaacattaaa aaataaacta tcaaaagaat ttttaaagat
     5641 ggaacaaaat ggagctactc ctgaagaact ggataaaaaa ggtacaggag ctttaagatt
     5701 tgcaacagta gatggagaca tagaaaaagg ttcatttatg gcaggtcaaa gtgctgctat
     5761 ggtaaaagaa ataacacctt gtaaggaaat tatagaggct atggtaaatc aagcaagaga
     5821 gattatgcca gcaatagaac tgtaaatcag gaggagcaag atgggaaaag ttgcactagt
     5881 attccctggt caaggagctc aatatgtagg tatggccaag gatttatatg aaaataatac
     5941 tgttgctaaa agtgtaatag atgaggctag tgatgtctta aatatggatt taaaaaacct
     6001 tatgtttaat ggaaatgaag aagaactttc taaaactgaa aacactcaac ctgctatggt
     6061 tacacatagt gtagcagtat taaaagcggt acaagctcaa atagatttaa aatataatgc
     6121 atgcttagga ttatccttag gagagtatag tgcgttggtt gcagcagatg caattgactt
     6181 taaagatgct gtatgtttag ttaaaaaaag aggtaagttt atgcaggaaa cagttccagc
     6241 aggtgttgga gctatggcag caattttagg tttagataga aatgtacttg aaaatgtggt
     6301 taaagatata caaggaggta ttgtagaagt agcaaattac aattctcctg gacaaatagt
     6361 tatatctggt gaaaatgaaa agatagaaga agcaatggta aaatgcaaag aagcaggagc
     6421 aaaaagggca gttaaattaa atgtaagtgg accttttcac tcatcaatgt taaaagaagc
     6481 tggtgtaaaa ttggccagtg aattagaaaa agttaacata actaaaccta aagttgatgt
     6541 ggtggcaaat gtaaatgcag attactataa aaacgaaggt aaagacctgc ttataaaaca
     6601 agtaagttca tctgttcttt gggaagattc tatagaaaga cttttaaatg atggatatga
     6661 tacttttata gaaatgggac ctggaaaaac tttaaaagca tttattaaga agatagcttc
     6721 caataaaaaa gcaaatgtca atatttataa tatagatggt atagattctc ttaatgaatt
     6781 tgtacaaaat tatagaaatg gggagatata atgataaatc ttacaggaca agtagcagtt
     6841 gttactggtg gctctagggg tataggaaaa gaaatagcaa aaaaactagc atcttttgga
     6901 gctgatgtag taatcaatta tacttctaaa gaagatgaag cactaaaaac taaaaatgaa
     6961 atagaaagta tgggggtaaa gtgtacctct ataaaatgtg atgtgtctaa atttgatgaa
     7021 gtgaatcaaa tgatagattc tgttgtaagc gaatttggaa aaattgatat attgattaat
     7081 aatgcaggca taactaaaga tggtctgctt atgagaatga aagaagaaga ttttgataga
     7141 gttatagata taaacttaaa aggtgtcttt aattgtacaa aagcagttac taaacctatg
     7201 atgaaaaaga agtatggaag aataataaat atgacttcag tagttggaat tatgggtaat
     7261 gcagggcaaa ctaattattg tgcatcaaaa gcaggtgtaa ttggatttac aaaagcttct
     7321 gcaagagagt tagcatcaag aaacataaat ataaatgcag tagcacctgg atttatagaa
     7381 acagatatga ctaaagtact aagtgatgat gtaaaagaat caacactagc aaacatacca
     7441 aagaaatctt atggtaaacc agaagatgta gccaatgccg tagcattttt agttagtgac
     7501 atgtcaagtt atataacagg acaagtaata aatgtagatg gtggaatggt aatgcaataa
     7561 taatttaaaa agtagagaaa tctattaaaa taaaaaattt taggaggtac acaatgttta
     7621 ataaagttgt agaaataata atagagcaat taggagtaga agataaagaa ataactatgg
     7681 agacatcatt aatgaaggat ttagaagcag attcattaga tgcagtagaa ataataatgg
     7741 cacttgaaga tgaatttggt atagaaatac cagatacaga agcagaaaac ttcaaatcta
     7801 taggagatat agttaagtat atagaagcta ataaatagtc gagctttaaa aaagtggagg
     7861 tatccgatga ataaaagagt agtaataaca ggacttggat gtgttacacc gttgggaaca
     7921 ggaaaagaag agttctggag taatataaaa tctggagtat ctggtataga taaaataact
     7981 aattttgatg ctagcacata tcaaactcag atagcaggag aagttaagaa ttttcatcca
     8041 gaagaatata taagtaaaaa agaattgaag agattagata aatttgctca atttgcaata
     8101 gtatctgcta aattagcagt tgaagatgca aatcttgatt tagataaagt tgatagagaa
     8161 cgttttggtg ttataatagg ttctggaata ggtggagttg aagccataga aactcaacat
     8221 aaaattcttt tggaaaaagg aaataaaaga gtaagttcac tttttgtacc tatgatgata
     8281 ggaaatatgg cagctggtca agtttcaata tttttaggag caaaagggcc taacactaat
     8341 gtatgtacag cttgtgcatc aggaactcat tcaataggtg atgcttttaa ggtaattcaa
     8401 agaggtgatg cagacattat ggtagcaggg ggttctgaag ctgctgtaac aggtcttgct
     8461 tttgctggat tctgtaacat gaaggctatg tcaactagaa atgatgaccc taaaactgca
     8521 tcaagaccat ttgataaaga cagagatggt tttgtaatgg gagaaggagc aggtattgtt
     8581 atcttagaag acttagaaca tgctttagca agaggcgcta aaatatatgc agaagtagtt
     8641 ggatatggtt taactgctga tgcataccat atgacaactc cagctgaaaa tggagaggga
     8701 gcagctaggt ctatgaatat ggctttgaaa gatggaaatg ttccattaga agaagtagat
     8761 tatataaatg ctcatggaac ttcaacttat tacaatgatt tatatgaaac tatggctata
     8821 aaaactgtat ttggagaaaa ggcatatgac ttatgtgtgt catcaacaaa atctatgact
     8881 ggtcacttat taggagcatc aggtgctata gaggctgttg tttgtgctat gagtatagaa
     8941 gatagttttg tgcctccaac tataaacata caagaagttg gtgaagattt agatttagat
     9001 tatgttccaa atcaaggtaa agaaaaaaat attagatatg cactatcaaa ttctttagga
     9061 tttggtgggc ataatgctac aatagtacta aaaaaatatg tataaataaa tattaaatat
     9121 aaaactaaaa aacaagcact tgttttaact gtataaattt atattagctt aaatttaaag
     9181 tctcctaaat tattttagga gactttttta ttaagattta agtctagcat atgttgtaaa
     9241 tcattttgat atattgtatt attaattatt aagaggttat ttatatatgc aagttttcat
     9301 tctacaatat acaaaaggag gactaaaatt ttgaatttta gactaccaaa gaaaaggtta
     9361 cccaaaaaga taatttttat atgtatagta cttattagta ttattttgtt tataaagtat
     9421 gaccaaaatg gattgaaaga aagagaaatt ataaataagg gaaaatatat gtctgaaagc
     9481 cttgtggcag ataaggagta tgatgaagca aaaaaagtgg ttaagacttc ttttgaatct
     9541 attccagaaa aaaaatacaa taaagtatat aatgaagttt gggatatgtt aaagactatt
     9601 tcatatgttc cagatggaac gaagatagtc attgattctt tagaaaagat gagagaatca
     9661 gatgtgaaat tatctgatga atgtagattt aatatagaaa aaagattagc atcagtgtat
     9721 ataatggata ataattattc taaggcagta gatttaactg taaagtctat aaagttggct
     9781 gagaaattgg ggaataatta tgaaaaagca agactagatg ttgatttggc gagcatattt
     9841 ttaaagatag gtggctatga aacaggagag aaattaataa aggattcttt taaaatagat
     9901 attgatgaag gtgaaaaaaa tggaatggtt agattatatg ctattataaa tctagcagaa
     9961 atatatgtag aagagggcga atatgataaa gctattgaaa taagtagcag gataaaagat
    10021 tataaaaagt ttgtaaatac ggataattac aatgattttg atataatggc ctctataatg
    10081 cagtcaaatg cgtatgtggg caaaaataat atacaaaagg caaagacttt tttagaaaaa
    10141 gctgattatc ttataaaatc agatagaaca gtttatatat tagataaaga tatgtactat
    10201 tatatggcaa tgggaaactt agaatataaa agcgaaaatt ataattctgc aatagataat
    10261 tataaaaaaa gtttagagat atctactaaa agaaagttaa cccaggagaa aataaaaaat
    10321 ttaaataaga tattaaatat atatgaaaaa caaggaaata tagaatcttt aaatgagtat
    10381 caaaagatgt taataaatga atataaagag aacaaaagta taagagattc agagactagt
    10441 ttctatataa ttgataaagt atcaaacgaa agtaagctat ttgaaaaaac taaaaaagaa
    10501 ataaaatatt ataaaatatt atttacagta attttaattg ctatattagg gtctactttt
    10561 ttatacaacc gattgaagta ttttaaagca caaaatttac atgatggtct tacaaatgtt
    10621 tataatagaa aaagttttga tattatgtat gaaaagtaca gagagaaaga tgataatttt
    10681 gcacttgtga tgatagatat tgataatttc aagctcataa atgataatta tggacataaa
    10741 tttggtgaca ctgttataaa gggaataaca aataccattt gtgttatgtt agaacaagat
    10801 gataaagtct ttaggtacgg aggagaagaa ttttctgtat taataagaaa taaatctggt
    10861 gatgaagtag cagatataat tgacagtata agggttgcaa ttgcaaataa aaagtggaat
    10921 gatgatgtga ctgtgactat aagtgcaggt gtagctcatg tttcagatag caaagatgta
    10981 ctagaagaag ctgataaaaa tttatataaa gcaaaacaac ttggtagaaa taaagtagta
    11041 tataagtaat tatatactat ataaataaga ggtataaata tgaaacaatc aatagctata
    11101 gtaactgata aaataactaa actatcatct ttcttagaag aaaatatacg tttggtttta
    11161 ggtaattata ttgatataaa ccactattat ctggaagttt taaaagagaa tgataaaata
    11221 caaggtgatt ttgtactagt aatgaatgat gatagattaa acagtatgag aaagcattta
    11281 gtacaagata gtaagattat agtgataagg agaacactta aagaaaatga aatatatgga
    11341 ttattttcaa taccaaatgg aactgaagta ttggttgtaa atgatacaaa agaaactacc
    11401 ttagaaacga taagcttatt ttacaaaata ggagtaaaaa atttaaagtt tactccatat
    11461 attgaaggaa aaatttataa aaatatagac atagctataa cacctggagt aaagggaaaa
    11521 gtaccaagtt atataaataa agtaatcgac cttggaaata gatatattga tatatctacc
    11581 tttatagaaa taataaatgg tcttagaata gatgttaaag aaatacgtaa aaatttaatg
    11641 gcatattctg agagtttaat aagtttagat aatggagtta aagataatta taggcaactg
    11701 tttttaaaaa tagaagaaca agatgttata ttgaatctat caaaagatgg aataatattt
    11761 acttcagcag atggtataat aaacacattt aataaagaag ctttaaaaat tttaggtata
    11821 aatgaaaatc cacaaggcaa aaacatagaa gatatcattt ctgaagattt aaatatttta
    11881 cttgaagaca aagaaataat tgatgaagtg gtatttacaa ataaaaaata tataaatgta
    11941 aataaaaaaa gtattttttc tatggggaat aaagctggga tatattataa cttacaagaa
    12001 ataacgtata taaaaaaatt agaacaaaat cttactaata aattaagaga acaaggtcag
    12061 atagcaagat atacatttaa agatataaaa actaaaaata aaaatatggt aaagtgtgta
    12121 gaattggcta aaaaaatatc taaatcagac ctttctgtat taataatagg agaaagtggt
    12181 actggtaaag aattattatc acaatcaatt cataatgctt ccaatagaag taatcaaccg
    12241 tttatagcag ttaattgtgc agcagttcca gatagtctat tagaaagtca actgtttgga
    12301 tatgaaaaag gctcttttac aggagcttta agagaaggaa aaaagggtct atttgaactt
    12361 gctaataatg gaactatatt tctggatgaa attggagata tgcctccatt gctacagaca
    12421 aagttactaa gggtattaca agaaaagcag gttatgccta taggttccca taatgttata
    12481 aacatagatg ttagggtcat agcagcaaca aacaaaaact tactaaagat gattagagaa
    12541 ggaaaattta gagaggacct gtattataga ctaaatgtat tgccaataga tgtaccatca
    12601 ttgaggaata gaaaagaaga tataataaca cttatgaatt tctttttgaa aaacaatata
    12661 agactttcgc cagaagttat aactatttta gaaaaatata attggccagg aaatataaga
    12721 gagttacaaa atgttgcttc ttatgtaagt ctaatgtgtg atagtatggt ttttagagat
    12781 gacttaccac catatataag ggtagaatgt gctaacaatt atgaagatgg atttggttat
    12841 acaagaaaag aatttgagga aatactgagg attctatatg aaagaagaga gtcatcctct
    12901 ggaattggaa gagggtttat aatcgaaaag cttatacagg aaggtattga gatttctgaa
    12961 agcaaagtta gaaaggtgct atcttattta agtgaatgtg gatacatatc atctaaatct
    13021 ggtagaggag gaagtcaaat aacctcagag gggataaaac tttataataa tataaattaa
    13081 gctaagtatt tgatattgaa atcacatatt ttgaatttag atatttaaat aataataaac
    13141 ttttatattt atagagatta atttatatta tgtcagaaag aatattgtaa tttataatta
    13201 aatattaata ggtttttaaa ataggattag ataggtaata ggttaagtta gaataaccca
    13261 ttacctattt ttatttgtaa gaaaagtgta catagaatta gaagtaaaaa atacaactaa
    13321 taaaactatt gaaaatacta gataaataat aaataaaaat aattatagat tttggcatac
    13381 atattgctaa ttatagagat aagacgacaa aataattggg aggtttaatg atgaaataca
    13441 aaacaacagg gacttgtgct acagaaattg aatttgaagt aaaagaaaat aaagttacaa
    13501 atgtaaactt tataggtgga tgtgatggaa atttaaaagg gctaaaagta ttagttgaag
    13561 gcatgaatat agaagatgta atacaaaagt taaaaggaat agagtgtaaa acaaagccaa
    13621 catcttgccc agaccaactg tctctagcat tggaaaatta tatgaatata aagtaataca
    13681 taaaacaatc gattagttag gagtttaatt atgaaaccaa taatagggat tttaggaaat
    13741 ttaataataa tggaaaatgg tatgtttcca ggtcttgaaa ggtcatatgt aaataatgat
    13801 tatataaatg cagtgctaaa aggtggcggt agtccagtaa taattcctgt aaatacagat
    13861 aaagaagtta taaaaaaaca gattgaaatg gttgatggag tcttaatatc tgggggatgg
    13921 gatatcaatc ctcaactgta tggagaagaa acaagagaag aaacgacatt tatatatcca
    13981 gaagtggatg aatttgattt aattgcaata agtatagcat tagaacttaa aaagcctata
    14041 ttaggtgtat gtaggggact acaaatttta aatgtaagtt taggaggaac tttatatcaa
    14101 gataataatc ttatagaagg aagttatata aaacatacac aatcttctaa aagacatgta
    14161 gctactcata aagttgatgt taaagaagga agtattttag agggaatttt aggaaaacaa
    14221 ttacttacaa atagttatca tcatcaaagt gtcaatcagc ttggaaaagg attaaaagcc
    14281 atagcatact caaaagatgg tataatagaa gctattgaga aagaggatga aaattttgta
    14341 gtaggagtac agtggcatcc agaaatgatg gtagattact gtgataaaat ggaaaaacta
    14401 tttaaatatt ttataaatat ttgttcgaat ttataatata ccttgggggg attgaacatg
    14461 aacgaaaaga acaaaatggg attaataagt attatacttt taggtattaa tgcagtagta
    14521 ggagctggag tttttctatt accaggagat gcaatgaaat catttggtgt agcaagtata
    14581 tttgtatata tatttgatat gttgttagtt ttatctatgg cattttgttt tgctgaggtt
    14641 gcaggtaaat tcaataaaaa tggtgctgcc tatgtatata caaaagaagc ttttggagat
    14701 ttttgtggtt ttgaagtagg tcttatgaaa tgggtaatag gatgtatttc ttggggagct
    14761 ttaatagtag gttttcctac atcattatca gcagtatggg ctccagcagg tgaaccacat
    14821 atacaaaaaa ttataattgt agctatgata gttgggctta caattataaa cttattaggt
    14881 gtatctctat caaaaattgt acaaaatgtt ataactgtag gtaaactaat tccattaata
    14941 ctatttatag gaataggtat attttttata aaaggagtta attttacttc ttcaacaatg
    15001 gtacctccag gagctggagc aacagagttt ggagcagcag cacttttaat gttttattca
    15061 tttacaggat ttgaatctat agcagttgca gcagaagata tggaaaatcc acaaaaaaat
    15121 ataccaatag caattataag tgtaatagta attgcatcaa taatatatat attaaatcaa
    15181 gttgtatgtg ttggtatact aggagattcg ttatctagta catctacacc agtggcagat
    15241 gctgcaagaa tatgctttgg aaatatggga gctggattgg ttacctttgg aacattggtt
    15301 tctgttggtg gaatttgtat gtgtggtgct tttgtaaatc caagaagttg tgtagcttta
    15361 gctgatgata aaatgttacc gagaatattt gcaagaaaag ataaaaaagg tacaccatat
    15421 gtagcgataa tagctactat gttaatcact ataccaatag ctctatctgg aagttttgct
    15481 gaattagcag caataagtgc agttgctaga ttcatacaat acataccaac atctttatcc
    15541 gtattagtgt ttagaaagaa aagaccagaa ttagtaggta cttttaaaac tccttttgga
    15601 gcagtgattc ctctaattgc tgtatgtgtt ggaacatggt tgttattcca agcatctatg
    15661 catcaattaa ttatgggatt aggagctttg gcaattggag taccattata ctttataatg
    15721 aagtcttata atagaaaagt ctatggtgaa aatttaaaga aaatagttta attatgttat
    15781 aacaatttat tgagttaaaa ttagacattg gcagaaaatt aaatacaaat aagtatctac
    15841 aaggctatat taaaatatta ttaatttttt attttaatat agccttatat tgttatcaat
    15901 aaatgaaaaa tattgtaaca acagaagaac aatagtatta tgtttataga caaaaaactt
    15961 attaggaggt aaatttatgc caacaataac attaaaaaat ggtgtagacg tacttattag
    16021 agaaggtgta agagaagatg ctcaaagcat tatagatttt tataatgaag tgggtggaga
    16081 aactcatttt ctgtcatttg gaaaggatga atataaaata tctttggagg aacaagaaaa
    16141 tgctatagaa tcagcaaaag catctgataa ttctgttaaa ctgattgcat ttatagatgg
    16201 tgaaatagtt gggattgcaa cgattgattc taatcaaaaa gcaaaaggaa agcatgttgg
    16261 agtattagga attgttgtaa aagaaaaata ttggggaatt ggattgggta aaaggcttat
    16321 gttagacctt atagaatggt gtaaatcaaa tggaataacc aagaagataa ctcttgtcac
    16381 taatgaagaa aattataatg ctataggatt atacaaaaaa gttggatttg aagtagaaag
    16441 catactaaag aaagaatgtt attataatgg tgtttatact gatttaatag gaatgtcact
    16501 tttattggga atataattag tgtacatttt atctcgaaaa aagtatatta agtatggtat
    16561 aattaaaaaa gaatataact taatataatt ttggaggacg aattatgaaa aatctgtgta
    16621 aaatatcaga tgattattta aacagcaagc ttaagtattt aaagttatta tcaaaacaat
    16681 atccaagtat atcaaaagca agtacagaaa taataaattt agaggctata ttaaatcttc
    16741 caaagggaac agagcatttt ataacagatg tacatggtga atatgaacca tttgtacatg
    16801 tattaaaaaa tggttctggt gtaataaaga gaaaaataga agaattgttt tcaaatacaa
    16861 taagagacag cgaaaaaaaa atgttagcaa ctcttgtata ttatcctgaa caaaagttag
    16921 acctaataat taagcaagag gaaaatatag atgattttta tagaataaat atttatagac
    16981 ttatagaact ttgtaaatat gcttctagca aatatactag gtctaaagtt agaaaactgt
    17041 taccagaaaa ttttaaatac ataatagaag aacttttgca tgagcatgtt aaaagtgaac
    17101 ataaagaaga gtattataaa agtatagttg aaacaatagt tgacatagga atagctaagg
    17161 agtttataat tgctatttct acagttatac aaaaattggt tgtagataga cttcatgtaa
    17221 taggagatat ttatgataga ggtccaagac cagatataat tgtcgataag cttattgaac
    17281 atcattgtgt agatattcaa tggggaaatc atgatatatt gtggatgggt gcagcctcag
    17341 gtgaaaaaac ttgtatagca aatgcactga ggatatcagc tagatatgcg aatttagaca
    17401 ttgtagaaga catatatgga ataaatctat tgcctttagc cacttttgct atagaaatgt
    17461 ataaagatga cccttgtaaa gaatttattc caaaggttaa tgaccaaagt gtaacaacaa
    17521 cagaaaagtc tttaatggca aaaatgcaca aagcaataag tataattcaa tttaagcttg
    17581 aaggtgaggt tatcagacga agacctgaat ttgaaatgga acatagactc ttgcttaata
    17641 tgataaacta tgatgaaggt acgattaatt taaaaggtaa aacatataaa ttaaaagata
    17701 cttatttacc tacaatagac aaaaaagacc catataaatt aactatggaa gaaagaaatg
    17761 ttattgataa attagtatct tcatttagag gtagtgaaaa actacaaaaa catgtttcat
    17821 ttttgttttc taaaggaagt atatatttaa aggctaactc aaatttactg attcatggat
    17881 gtgtaccgtt aaatgaagat ggaagcttta tgtctatgaa tataatggga aaagagtata
    17941 aaggtaaggc tcttatggat aagatggagt ctctagcaag agaaggattt ttctttaaag
    18001 ataaggcaga agaaaaactg tatggcatgg atattatgtg gtatttatgg actgggaaat
    18061 gttcatcatt atttggtaaa gatgatatga ctacatttga aagatacttt atagctgaaa
    18121 aagagactca taaagaaaat aagaacccat attttaaact tagagaaaat gagatggctt
    18181 gtaaaagatt atttgaagaa tttgatttag aacttgatga atctcatata ataaatggtc
    18241 atgttcctgt ggagagtaaa aatggagaga gcccaataaa agctaatggt aaaattctcg
    18301 ttatagatgg tggtttttct agggcttacc aaaaaactac tggtatagct ggatacactt
    18361 tgatatacaa ttctagaact ttgcaattag tatcgcatga accatttaat tctgctgaag
    18421 aagccattgc aaatgaaagt gatatcttat cgacaactgt tgtagtagag cataaggcaa
    18481 agagaaaaat ggtgagagat actgatgaag gaataaagat acaagaggaa atagaagatt
    18541 taaaactact tttaatggct tataagaaag ggctaataaa agaaatgtag atgttataga
    18601 ggcacttcat gattgaagtg tctttttgtt aatacttatt aaatatatta taaagattaa
    18661 taatagtagt ttatattata cttactaagt gaatccaaga taaatgggtt cacttttttt
    18721 atttataaac attattaaaa aaatatagat gttttggtta tattccttgt cctttataaa
    18781 tagaattata taaacaaagg aggaggacaa gagatgaata aactttctga tgaaataata
    18841 aactctcttt ctacaactat aagagaaaag gtagaaaaag tctcaaacaa taatctgaac
    18901 atagaagaaa ttaggttacg ctcacaaaaa ccattgatac taaatgcaaa ctctaaagat
    18961 tatttttaca atcaaaaaac tatgacactt gatttaaatc aacaaaactc atatgtagta
    19021 acaagagaag atgtagagca aacttttcaa ataatatgta aatattcaat acattcattt
    19081 atggatgaca taaaaaaagg attcataact ttgagaggag gtcatagagt agggttagtt
    19141 ggaaaagcaa tagtagaaga tggacaagtc aaaaatataa agcatatatc atctttaaat
    19201 ataagggtat caagggaaat tattggatgc tcggataaga ttttaagtca tatcataaag
    19261 ggaaaaaatc agattaataa tactttgata atatcaccac ctcaatgtgg aaaaacaact
    19321 ttaataagag atatagtaag aaatttaagt aatggtaatg aagattatgg atttaaaggt
    19381 ttaaaagtgg ctttagtaga tgagcgtaat gagatagcag gagcttatct aggtgttcca
    19441 caaatggatg tgggaataag gactgatata atagaaactt gcccaaaaga cttgggtatt
    19501 acaatgcttt taaggtctat gtcaccaaat gttattgtga cagatgaaat tggaagtgaa
    19561 aaagaaataa aggcattata cacagcatta aatggaggta taggattaat aactactgtg
    19621 catggagatt caatagagga tatacaaaat agaaaagaat taaatagact tctggataaa
    19681 gagttattta aaaaggttat aattctttct gcaaaaagag gagcaggaac aattgaaaaa
    19741 atttatgatt tagaagaaaa gagatggtat tttgcaaatt aaaataatta ttatagcttt
    19801 cttgatagga agtagttatt tgataggtga acaaatatac aaaacttata ctcgtagaca
    19861 taaacaatta aacgatttaa taagagtttt agaaattttg aggatggatt tatcttttgg
    19921 actttataca ttagaagaaa tctttaatag gataggtgga aataaagagt tttgtttttg
    19981 gaaatttttc tatcaaatat cagaaggact tcacaatgaa caaagtaaaa ctttggaaat
    20041 aataatatca gaaaatattg atgtcttaag taaagaaact tatctaggca ataaagaaat
    20101 tgaggaactg aaaaatctaa tacttacttt agggaaaagc gatatagagt cacaacaaag
    20161 aatgatagat ttatctatag aaaatttgaa aaaacaaacc tatgagacaa aggaagatat
    20221 aaacaaaaaa ggcgtattgt ataaaaaatt agtgacattc ataggtatag gtatatgtat
    20281 tatattgatt taatatgggg aggtttatta tggaaatatc attgatatta aaggttgcag
    20341 gtgtcggtat tctaatatca gtattgaata tgatattgga aaaaacagat agaaaagatt
    20401 gggcaggtct tactacatta gcaggtgtaa taatcgtatt gggtatggta ataacagaaa
    20461 taagtgactt gtttaatact gtaagaacaa tgtttcaact ttactaaaga ggagggagta
    20521 atttggaaat aatgcaatta ataggaattg caatcatttc aactacactc tgtttagtaa
    20581 ttaaaaaaga tagaccagag atagcaaatt tcatagctat aataactgga gtaataatac
    20641 tattgtcagt tatgtttaag ctaaatttta tagtagatag tatacaagat ttggctaaca
    20701 aggctaacat cccgactatg tatatatcat taataataaa attaattggg atagcatact
    20761 taatggaatt tgcgatacaa ctttgtaaag attgtgggga aggaaacatt gcttcaaaac
    20821 tagaatttgg aggtaaaata atagttatgt caatgtcttt ccctatactt ttgtccattg
    20881 tagaaatggt ggtgaatata attccataga tgtgtatctt ggaatgaata taagtatatt
    20941 agagagcttt cgttgtattg aggtttatag agtgaagaga ggcatggttg cttatgaaaa
    21001 aaagattttt accaatgatt ataggttttt tatttacttt cttttttgta aatatctttg
    21061 ctattatgat atttgctaat gaagtaccaa gtagtgaaga taaagaatat ggtgaaacaa
    21121 aaaatagcat agataagtat atagatggtc aattagacaa gctagatata aatgagattc
    21181 aagactatat aaataaagaa atagttatta atgatgttaa tttaaaatca tttgtaaaag
    21241 atttaattag tggagaaaaa aatatcttag atttattcaa taaagatgga ttgaagatat
    21301 tgatgtttga tgaatttaaa gcaagtttaa aggtagtagc agtaattttg gtactggctc
    21361 tgttatcatc tattttaaag agtctggaaa actctttttc atcaggtgca gtaagtcaaa
    21421 tagcaacata tataatattt atcacaatgg tatcacttac acttattggt tttaaagatg
    21481 tattacagat ttgctatgat gcaatagacc atactgtagg tcttatgcaa gttataatgc
    21541 caatattaat aacattttta cttttaattg gattcccaat aacgtcgact actttgaatc
    21601 ctatcttcat aggaggagta acatttataa atgtattttt taaaaatttt ctatttgtat
    21661 ctattactgt tgcatttgga attttaataa taaacaattt atcaaaaaat ataaggctaa
    21721 agagattttt ctcctttgta aaacaaataa attatgtttc tattggagct atgtttactg
    21781 tttacttagg tcttgtatcc atacaaggac tgtatgtaac cagttttgat aaattcagtg
    21841 taaagactgc taaatttgct ataggtaatt ttattcctgt tgtaggtgga tttgtatctg
    21901 actctgtgga tattttatta tcttcatctc agcttataaa aaatatattt ggaggaatag
    21961 ggcttatctt acttgttgga atttgcttac ttccagtaat aaaaatttta tcagtaatag
    22021 tagtgtacaa attagcagca ataattgtag agcctgttgg agaggatgga atatctaatt
    22081 ttttaaatga agtggcaaat ttaatgataa taatgctagc atcagtaatt gctataacag
    22141 taatgttctt tgtaactgta gctatcttga cctcgataag tgtcgtaagt caagggtaat
    22201 ttatatatca gtgaaaaata attacattta atctaatgtt tttagagagg aggatgctat
    22261 gttagagggc ataaaggcat ggatagttag tgttttgata ggagcattta tagtaaatat
    22321 agtagatatg attttaccaa gttctaagat aaaaccttat gtcaacctag ttttaaactt
    22381 tatgtttgta tttattgtaa taactccagt agtaggattt ttttcaaaag atatgagttt
    22441 agaagataga atattaaaat ctatgggaaa ctacaacaag cagtatgtag atagtacaaa
    22501 tgctttggca aaagagactg gaaataatag tctatccaag ggatatgagg atgggttaaa
    22561 agaagtactt aaattaaaac ttgatgaata tggttatgat ttagaagata tagagcttaa
    22621 tggggcaaat ataaataata taaaaataaa agaaaagaat aatagtgcaa aaagtaataa
    22681 cagttccaat gaagaaaata aaaataatag tactaaaaga gaggaagaaa acataaactc
    22741 taatgacaaa gaaaattcta aacaagtctt caaaaaaggt acagagtatg ggttgaacct
    22801 aaatgaagaa aaattaaaaa acgacttaat aaaggtgctg gatgtttcta tagaagatat
    22861 acaaattgat aaataggagt tgggagaatg ttgaaaaact taaatgacaa agataagaga
    22921 aaggtatatt cactaataac tatagcagga atatgtgtgg tttcattagt attattatct
    22981 tgttttccta gtagcaaaag cgataaacaa gttggaaaaa cagagactaa taaaaatgct
    23041 gaaaagcaag tcactaaaga acaagaaaaa gatgatttag agtcaaaatt aacagcaata
    23101 ctatctaaaa ttgatggtgc aggagacgtt gatgtaatgg ttacatttga atcaagtgaa
    23161 gaaatacaac ctgcttttaa ttcaaataat acaacagaaa caacagagga aaaagatgca
    23221 caaggtggag aaagaacagt tacgacttct agtgaaaata aaacaatgat aacttcaaat
    23281 tcaagtgacc ctgtagttat aaaaactaca gagccaaaga taaagggagt gatagtggta
    23341 gcaagtggag caagtgaccc aagtgttaaa gagacacttt atagtgcagt acaaacatca
    23401 ttacaagtag ctggtcatca agtagaaata tattctaaat aaatacatat aaccgggggg
    23461 ataaatgaat gaagtttaat tataagggaa gaggatttgt aataataact ttaactgcaa
    23521 tgttagtggt agtaggaaca gtgaattatc aactaagtaa gaaatcttta ttagaaacat
    23581 caaaagagtt taaggcatat gaagaagcac agttgcaaaa aaataatgat gagagtgata
    23641 gtagtaagac agaggattct agtaatatag ataaacaaga tggtaaggaa agtgctgata
    23701 ttgatatagt tgatagcaaa gctagtaaag taaaagaaaa ggcaactgag acaagtaagg
    23761 aaataaaagc tcaattatca tcagagaaaa atatgaaaaa ggcatcttat attttagata
    23821 tgaaaatgaa tagagaaaaa caaagaaatg agttagttca agatttaaat gaaatgataa
    23881 ataatccatc tacaacagaa gaatctagaa aagaagcttc taatatgaaa ttaaatatag
    23941 ttaaaattca agaaaaagaa cttcaaatag aaaacctttt gagtacaaaa gggtatgaag
    24001 aagcgttagt ttatataagc gataacaaag ttaatgtagt tgtaaatgaa gctaaattag
    24061 aaaagaaaga tgcagcaaaa atatttgatt tagttgcaga acaagccaat gtgaagtatg
    24121 aaaatattaa acttacaaat aataatagta ataagtaaag tataaacctc agaattaact
    24181 gaggttattt tcttgcaaag tagacttgta tttggtatta ttatacttat aaaaatagtt
    24241 ccttaaaatc aggaggtaag ttttaatatg gaagataata aatttggaca agtaaaaata
    24301 tctaatgatg taatagctac aatagctggt ttagcagcat tagaagttga aggcatagaa
    24361 actacagcaa cattaacaga taagttgtta aaaaataatg gagtaaaaat acaaatcgaa
    24421 gaagaggatg ttaatttaga tgtaatggtt acgataaagt atggaatgtc aataccagat
    24481 acagctttta aagttcaaga aaatgtaaaa aatactgttg agacaatgac aggactgaag
    24541 gtttctcaag taaatataca cattcaagga atcagcttta agaaggataa agttgataaa
    24601 gaagaagcaa aagcaactaa gaaaaattaa tattagccct ctgaaaagtc agagggtttt
    24661 ttgctggtaa ttgattttat atggataatt aaacttaaag gagaattttg attgatgaaa
    24721 aaagataggg cacaaaagag tacaacaaga gaatatataa tgaaacttat ataccaaata
    24781 aatattaata aagaagattt tgaaacttta gaagataaag tagataattt tttaaaagat
    24841 aattcagaac atattataaa tagatataaa gaacttgcac ttcaatactc aaaaaataca
    24901 aatttaaagt tagaagatac tgaaattgaa gatgttatag acaaaaaata tataaataca
    24961 gtatgcaaag cattgaaaga aaatcatgac aaaatagatg agttaataaa taagcatgct
    25021 aaaaactgga cagtagatag aatgccaaag gtagatgttt ctatacttag attgtctgtg
    25081 tgtgaaatac tttatttaga cactcctaat aaagtatcaa taaatgaagc agtagaactt
    25141 gcaaaaatat attgtgatga taagtctcct aaatttataa atggtatatt gggaagtgtt
    25201 gttgatgaaa ttgggaaata atataataat tggaattgat actagctgct atactacttc
    25261 tatagcagct atctctttag ataagaaagt tatattcaat gaaaaaataa tgttagaagt
    25321 aagggataat tcaaaaggtt taaggcaaag tgaagctgta tttcaacata tcaataattt
    25381 agggattctt agtgatagga taaaatcttt taaagataag tttaatgtag aaggagtttg
    25441 ctcatcaaaa aaaccgagac ctgttgaaaa ctcatatatg cctgtattca atgtaggtca
    25501 taattttgga aaattattgt ctagtatata tggttgtagg ttttatgaga ctactcatca
    25561 agagaatcat attgaagcaa gtttattaaa tagtaaatta aaaaataata ataaatttat
    25621 ttctgttcat atgtctggtg gtactacaga gatactttta acaagcaaac aagatagtca
    25681 tcacaatgta tgtgatacta atttaggtaa aattgccaaa ataagtatta aaaaagatga
    25741 taaaagtaag ttatacaata attttggata taatatagat ataattggag gaagtaaaga
    25801 tattagcttt ggtcaactta tagatagagt aggaattaag cttggatata aatttccttc
    25861 aggaaagtat ttggatgaaa atgctttaaa ttgtaatctt aaaatagaaa gtggtctgaa
    25921 aacttctgta agagatgggt atatgaattt atcaggatta gaaaatcagg taaataagat
    25981 tataaatgat aatggatata atactaatca aaaagaatat atatctaaat tggtattaga
    26041 ttcagtagtt agaaatatgt ttaaatcttt agtgtattta tgtgaaactt ataatgtaaa
    26101 tgaagtagta tttgctggtg gagtttctgc aagtaagtat attcttagag aattaagcgt
    26161 gaagttaaga aaaaagcata tagaagctta ttttacagaa ccacaatatt caactgataa
    26221 tgctgtcggg tgtgctataa taggattgaa taacttttta ggagaaagag tatgaaacta
    26281 agagctttag atataagtga agcaaactca tatattaaaa gaattttaat aaacgaccca
    26341 atactttcta atcttaaagt taaaggagaa atttctaatt ttaaggttca tagtagtggg
    26401 aatgtatatt tatcacttaa agatgaaaca tcaaagttaa actgtgtaat ttttaaaagt
    26461 aattttaatc gcaatttaaa attagataat ggtgtaaaaa ttatagctaa tggatatata
    26521 tcagtctatg aaagagatgg tgcgtatcaa ttatacatta atgaaattga aatagaggga
    26581 ataggaaatt tacacataga gtttaataga ttaaaggaaa aacttaataa agaaggatta
    26641 tttgacccta aatacaagat acctatacct aaaatgccaa actcaattgg cgttataaca
    26701 tctccaacag gtgctgttat aagagatata ataaatgtaa taaaaagaag atatccgaaa
    26761 gttaatataa agctttatcc agtaacggtt caaggagata aatcagctga agaaatctgt
    26821 gaagcaatta gatttttcaa tcatatgaag aatgttgata cacttatagt tggtagaggt
    26881 gggggttcga tagaagaact ttggtctttt aatgatgaaa tggtagcaag agaggttttt
    26941 aattctcaaa ttccgataat atcagcagtt gggcatgaaa cggattttac tatttgtgac
    27001 tttgtgtctg atatgagagc cccaacgcca tcagcagcag ctgagattgc tactccatca
    27061 ttggatgata taaactataa acttggaaac ataaaaagta ggatgagtaa gtctcttaca
    27121 aatcaaatag agctagacca atataggtta gaaacagtat ttaataagat aaataactat
    27181 ttagattcgt acacaataaa agataaagtt atacaattag acaaaatata tgataaaata
    27241 atttttggaa tagaaaataa tttaaaatta gaagatgaaa aattagtaaa aattggagca
    27301 ttactccata atttaagtcc attagctact atggatagag gttatagtat aactcaaaaa
    27361 aatggaaaag taataaatag tataaaagga ttaaaaataa aggatagtat agatatagta
    27421 ttaaaagatg gaaatttaga atgtatgata gataaaattg aaaataaaga gggatagtat
    27481 gaatttaacc tacgaagaag cttataaaag attagaaagt attttaagtg aattagaatc
    27541 taaaaatgct agcttggatg aatctttaag cctatatgaa gaaggtataa gcctttataa
    27601 acattgtaat aagcttttag atgatgctaa attgaaaata agtaaattta atcaattggg
    27661 aatagaagaa gactttaaaa tagaggagga ataaaattgg aatttaaaca atgtctaaaa
    27721 gaaaaagcaa gttttgtaga aaaagtactt aaagaatata tgcctaaaga agaagggtat
    27781 caaaaaactg ttattgaagc tatgaactat agcttaagtg ctggaggtaa aagattaaga
    27841 ccaatactta cattggaggc ttgtaaaata gttggaggta atgaagatga agctattcct
    27901 tttgctattg caattgaaat gatacataca tattcactaa tacatgatga tttgccagct
    27961 cttgacaatg atgatttaag aagaggaaga ccaacaaatc ataaagtata tggagaagct
    28021 atggggattt tagcaggaga tgctttgtta aactatgctt ttgaagtaat gcttgcaggc
    28081 tcaatcaata aagaaaatcc agaaaagtat ctaaaagcta taaatgaaat tgccaaaggt
    28141 gctggtatat atggaatgat aggtggtcaa gtagtagatg tagaaagcga aaataaacaa
    28201 attgaaaaag aaaaattaga ttatatacat atgaataaaa ctgctgcaat gatggtaggc
    28261 tgtatgagag caggagctac tataggtgga gcaaattctg aacaaatgga agaaataact
    28321 aaatatgcaa aaaatatcgg attatcattc cagatagtag atgatatact agacatagta
    28381 ggagatgaag ctaagttagg taaaaaggtt ggaagtgata tagaaaatca taagtcaaca
    28441 tacccatctc ttttaggttt agataaatct aaagaaattg cacataattt gattgatgaa
    28501 gcaaagaaaa gtatagaaaa attatctgat gatgtagatt ttttaaaggg attggctgaa
    28561 tatataatag atagagaata ctaaaatata cagctaaaat cgaggaggga aaaatggatt
    28621 ttttttcgga gatttttaac aatggggctt tgggaataag tctgattgct tgtttcttag
    28681 cacaatttat aaaaatattt actggtaagg aaaaaagaat tgagttatca agaatactta
    28741 tttcaggagg tatgcctagt tcccatagtt cttttgttac aagtttagct acagtggtag
    28801 gtattgaaaa agggtttaat tctactgatt ttgctataat taccgtgctt gccttaatta
    28861 taatgtatga tgcagcaggt gttagacggg ctgttggtaa acaagctact atattaaatc
    28921 aaatggtggc agatatacag catggaaagc atatagagca gaagaaatta aaagaattaa
    28981 taggtcatac accactagaa gtttggtttg gagctttact tggaatagta acagctttaa
    29041 tattgatgta aattgaaaat ttacagaata aaggtgagga taatatgtat aaatatttag
    29101 ataaagtaaa ttctccaaag gatataaaaa atatgagtat agaagaaatg gatttacttg
    29161 caaaggatat aagaaagttc ttagtaaaat ctgtttcaaa gacaggagga cacttagctt
    29221 ctaatctagg agtggttgag ttaacattag cacttcataa agtatttgat agccctaaag
    29281 ataagattgt atgggatgta gggcatcaat cttatgttca taaaatagtt actggaagaa
    29341 aagattgttt tgtatctttg agacagttta atggactaag tggttttcca aaggagaatg
    29401 aaagtcctca tgatattttt gatacaggac atagcagtac atctatttct atagctacag
    29461 gtattgcctg tgctagggat ataaaaaaag aaaactatag tgtgatatct gtgataggag
    29521 atggttctat aacaggaggt atggcacttg aagctttaaa tcaattagga tatatagata
    29581 ccaatatgat agtaatcctt aatgataatg agatgtccat agataaaaat gttggaggaa
    29641 tgtctaaata tctgtcaagt attataagaa actcaacagt tgagaaaatg acagatgaag
    29701 ttgataaaat tttaaatgtt acacaaacag gtgaaatttt atctaagact gcacatagat
    29761 ttaaagataa actaatgtat agcttttctc ctcaggactg ttcatttttt gattcattag
    29821 gtataagata ttatggacct atagatggcc ataatactaa agaactaata gatatattga
    29881 gaaaagctaa acacaaaaaa gggcctgttc ttttacatgt aattacgaaa aaaggaaaag
    29941 ggtacagatt tgcagaagaa caacctgata aatatcatgg agtatcaaag tttgatataa
    30001 agacaggtgt tacatctgca aaagttaagt ctatgtcaat tagtgttgga gaaaaacttg
    30061 ttgatatggc taatagtaat gaaaatatag tagcaataac agctgcaatg ccatctggta
    30121 cagggctaaa cctgtttgaa agtgcatatc caaaaagata ttatgatgtt ggtatagctg
    30181 aacaacatgc aacaggattt gcagcaggtc ttgcaaaaaa tggtatgaaa ccttactttg
    30241 ctgtgtactc atcatttttg caaagggcat atgaccaagt tattcatgat gtatgcataa
    30301 caaaaaagcc agttactttt cttatagata gagctggatt agttggaaat gatggagaaa
    30361 ctcatcatgg tatgtttgat ttaagttact taaattctat tccaaatatt gtagtaatgg
    30421 ctccaaaaga cacaagagag atggagctta tgatggattt gtcattaaaa ttagattgtc
    30481 cactagcaat tagataccca agaggaagta gttattacct agataaggga gagtatggag
    30541 agattgtact gggtaaatat gaggtattag atgagggtca agatacagtc atactttgta
    30601 ttggaagtat ggtaaaacat gctttagagg ctaaagaaat attatcaaga gaaggaataa
    30661 atccgacaat agttaatgct agattcttaa agccaattga tgaaggtatg ttaaaagcat
    30721 tattaaaaaa tcataaaaat gtagttacta tagaggataa tattgtaact ggaggatttg
    30781 gaagtagaat aaataagttt attatagata atgaatataa cgtaaatata cttaatatag
    30841 caataccaga agaatttgta aaacatggca atatagatga attatatgac tttgtaggat
    30901 tatcgccaaa aagtattgct gataagataa ggaaattagt tattgagtaa aaatctaatg
    30961 tagatattgt aaattaaaat ttatataaaa tatagaattt atcaattaga ataaatatca
    31021 agtgtgttac ttcaagctac tttagttatc attaaagtag tttttaacag aaaggtaatt
    31081 gtatgaaaaa aagaatagat ttattattag tagaacaagg gtactttgaa agtagagaaa
    31141 gagccaaaaa agcaataatg gctggattgg tttttgttga taatcaaagg tgtgataagg
    31201 ctggtacaga agtaaaagaa gattgtagta tagaagtaaa aggaaatcca ataccatatg
    31261 tcagtagagg tggtttaaaa cttgaaaaag caatgaaaaa ctttgatttg actattgatg
    31321 gaaaggtatg tatggatata ggggcatcca caggtggttt tacagattgt atgctcaaaa
    31381 atggagcaat aaaagtattt tctatagatg ttggttatgg tcaacttgca tggaaattga
    31441 gacaagatga tagggttgtg tgtatggaaa gaaccaatat aagaaatgtg acaatagaag
    31501 atacaaagca gtttgctgat tttgcgtcaa ttgatgtgtc ttttatatcg ttaaagttag
    31561 tacttccaaa ggcaaaggag ttagtacgtc atgatgggga agttgtcgct ttaattaaac
    31621 ctcaatttga agcaggaaga gaaaaagtag gtaaaaaagg agtagttaga gaaaaatcta
    31681 cacatataga agttataaaa atgatttctg atttctcagt cgaaaatgga tttgagattt
    31741 tagggttaga tttttctcct attaaaggtc ctgaaggtaa tatagaatat ctaattcatt
    31801 tgagaaatgg aaatgaagga tatgaatttg atggtgagac atacaataac aaaatagtag
    31861 aagttgttga agcttctcat aatttagaca aataggttaa aagggggctt agatttgatc
    31921 ctcgaattat atatgaaaaa ttgtgcctta gttgaagaac taaggttgaa tattgataaa
    31981 aacctaaata tacttacagg tgaaactggt tcaggaaaat ctataataat agatgcactt
    32041 ggactttgtt taggtgataa atatgatagg tcttttttaa gaaaaggaac agataaggga
    32101 cttgttgagg ctgtattttt ttcagacaat agatatctta aaaaaatatt agaagaaaat
    32161 gatataagta tggaagatga taatttattg gtaataacta ggttgatata ctcagatggt
    32221 aagagtactg ctagagtaaa tggaaggact gttaaagtgt ctcttcttaa agaaattgca
    32281 tctacactta tagatataca tggtcaacat caaaatcaag ctttgtttaa taaagatact
    32341 catttaaaat ttctagacct ttttggagaa aatgaattgg aagagtttaa aattgcttat
    32401 aaaaatgtgt accataagta tagtgaagta aaaaaagctt taaattgctt gacagaaaat
    32461 aaagatgaga tgcaaattca acgagaaata gatttattaa gatttcaaat aaatgaaatt
    32521 gaagctgcaa atctaaataa gaatgaatat gaagatttat taaaacaaag agaagtttat
    32581 agaaatagtg aaaagatata taataatcta aattcgagtt atagcaagtt acataatggt
    32641 gagtataatg ttattgacct aataggtctt gcatctaagg aacttaatga catatctaaa
    32701 tatgacagtg ttttaagtga atatagtgat actgtagaaa gaatcatgta tgagttacaa
    32761 gacatatctg gtgagataag aaattacaaa gataatatag actttgaacc ttatgagcta
    32821 gagcagattg agcttagaat cgacgagatt aataacttaa gaagaaaata tggagacagt
    32881 atagatgata tatttgagta ttatggaaaa attaaagata gattagatga aatattaaac
    32941 agagatgaaa gagttgaaca gttaagaagc aaactgatga atatagaaga agatttaaaa
    33001 ataaaagcca gtaaattgac taaagcaaga atagaagtag caactacttt agaaaaaata
    33061 cttcttgatg aattaaaaag cttaaatatg aaaaatgtaa tgttcaaggt taattttgaa
    33121 aaaggtccat ttactttaga tggagtagat gatattgaat ttatgatatc ttttaactta
    33181 ggagaagata ttaagcctat atataaagtt gcatcaggtg gtgagatgtc cagatttatg
    33241 ttagcgttta aaaccatatt ggctgatata gatgatattg atacattagt gtttgacgag
    33301 atagatactg gaataagtgg gatagcagca cagatagttg gtgagaaatt gagtgatata
    33361 gcaaagaaaa aacagataat atgtataact catttacctc aaattgctgc aaatgcagac
    33421 actcattatt gtatagagaa agatacttca aataatagga cgtttaccaa tgttagtaag
    33481 ttaaatgaat ctcaacgaaa aaatgaaatt gccagactta tagcaggtaa caatataaca
    33541 gaaaaaacta ttgaacatgc aagtgaaatt attgagttgg ctaaaaaatg ttagtgaaaa
    33601 acaagcattt taataggtgt aaagaatttt tatagaaatg ctttacatct attttttatg
    33661 atgaaattat tttaaaatat ctagtgccta tattgaagaa aagtagattt aaaaatagaa
    33721 gaaaatttgt ataaatttgt aactttgaaa tttctaaatg cttaagtata atattattta
    33781 ttaaggtagt agtgaggggg acataaaatg aataaaaaag atactatgca cttaattttt
    33841 acaattgtaa tttttatttt attatggtat attgtggtaa cggtaagtga gattaataaa
    33901 aatattgaaa gttgtagtaa taaattagat tatttgtcac aagagattat gagtactaaa
    33961 tcggatatta gtaatactat aaatgaagaa atgtcaaaaa gttatataac aaagagtatt
    34021 gacttaaaag taaaaaaaat cgagaaaaaa gagtgtgtta ttgatgtaga tgttcaatta
    34081 agtaaacttg gaaaaaatgg aaaagtattt tttatgtata aagaagatag tgataaatgg
    34141 aatgaaactg agatgacaaa acatggagaa ttatcatatg catgtgaaat aaagataaat
    34201 acaggaagtg aatatgacta taaagtagtt acaagtggag ctatatctga aagttctgat
    34261 gtgcaaaaat tgactaaatc tgattatatg cctgaacaac ccatatttaa ttctgggata
    34321 agagataata gagaatactt tattgaaata gtagaaaata gtaatctttt caatcatgag
    34381 agtgaaaagt caaaaaataa agtttatgat aatattagat tagaaaaagt agatattatt
    34441 gtaaatgaag gaaaaaaaga cactatatac aaagccaaat tagctgaagg cttagatgat
    34501 ggcaaaacta ataattcaaa tagaaatcaa gtgaattatg aagtaagttt tcctaaaagg
    34561 gatattgatg gcataattta tattaaagct aaacttacat acaataatgg agtagaatat
    34621 acaaaggata taactgaaga tataactatg ggattacaag atttagaaaa ataaatacat
    34681 atttaaataa atattcaaat agatttgttc aataaaatag agtataaata aaactgtagt
    34741 agttttactt atactctatt aaaaaattat aattttaatt ccatatatct agccccttca
    34801 attggattat aagtataagg ttctatatca taaaaaccaa acttttcata taatccttgt
    34861 gcacttttca tgcttggaag agtatctaat ctcatgtgtg tatatccaaa ttttttagct
    34921 tcttcaatga tttcttccaa tagtattttt cctattttta atcctctaaa ttgatttctt
    34981 acatataatc tttttaattc acatatttta ccttcaagtt tttttaaagc aacacaacct
    35041 gccaaatttt catcaacaaa tgctaatatc aatgagcctg aaggtttttt atattttcct
    35101 ggaagtgttt ttaattcatt attaaagtct tgaaaacata agtctatatt tagagaattt
    35161 gaatattcag taaatagaag ttttacgttt tcaaggtttt ctaaatcttt ttcttcaaca
    35221 aattgtaaag ttatattttg agccatttta tccgccatta tataaacatg tttatataat
    35281 gacttcacct ccttgatata tgtatatttt tcttaatatc aattagattt taataccatt
    35341 actttttcac caccatagtc aagctctcca ttaaactcaa atccaaaatc tttataaagc
    35401 ttaatagcca ttttattgtc ttcgaatata ctaagatata gttcatcaca gttatattca
    35461 tttttaagat gatttacaag aaaatctaat gatgcttttc catatccttt accttgatat
    35521 tttgaatcaa tcataaatct atcaagccaa actctatcat cttctaaacg accatacata
    35581 gcaaatccta ttaaagaatt ttcatcatat attccaacag gtacccaagc tgttataaat
    35641 ttagattcag caatagaaag tgcattgctt tctatataat ctaactgact tttatctaca
    35701 gacaaactaa ctaaatcatg ccaattattt atgtctgcaa ttcttacatt taatttcaag
    35761 atattaccgc ccttcaaact ttttgttaca atctaatact atcatattat gtattaaaaa
    35821 tcacttatta agattttaaa taaacttttt aaatgaataa attctcagga aaaaggaatt
    35881 ataaatacat aataatacag cataggagta gatggatata tgaacaaaat aaataaaaat
    35941 ttcaaaaata tttggataat attaggagct gtatcgattt ttaccatggt tataattttt
    36001 aataaaaatt gtaatgttaa taaagagact caaatagaag cttttacaat taagaatcat
    36061 gataaaaaat ttgtatatcc tatgggaaat ataattggag taaaagcaaa tacagatggt
    36121 gtattggtct taggatatga ggaagaggat gtagactata ttggaggcat acaaatcggt
    36181 gataatatag taaaaataga taataaaagg ataaaaaata gtcaagatgt atcagagatt
    36241 ttgaataaga ttaaaaaaag taaagttgaa gtgacttttg agagaaaaaa taagtataaa
    36301 acagaagtaa tagagactaa aaaagaaaat ggcaagtata aacttggatt atgggtaaga
    36361 gataaaatat ctggaattgg aactatgact ttttacgacc ctagtatgga aaagttcaaa
    36421 gcaatcggtc atgccataaa agattcggat actaatgaac tattaaagat aaagcaagga
    36481 tatatatata agccagaaca attaaaaata gtaaaagcta gtaatgaaaa agtaggtaaa
    36541 ataaaaggag attttaatga cagcaattta atgggtaatt tctcaaataa ttcagagtta
    36601 ggcataagtg gtaatattac agaaaaccat aataaagagt ttaatgtagc aaataaagaa
    36661 aagcaattaa tagaagttgg aagaccgcaa gatgtaaaaa taggagatgc agtaattctt
    36721 tttgaggata aaaacaaaaa tataacaagc tatgatataa aaatagaaag tatagtatat
    36781 gataaaggaa attatagaga tatggtaata aaagtagtag atgacaagtt attggaatac
    36841 acaggaggta tcgtacaggg gatgagtgga gctccaataa tacaaaataa taaaattatt
    36901 ggtgcaataa ctcatgtttt tagagataat ccgaaaaaag gatatggtat ttttatagat
    36961 gaaatgataa aattgtaggt gaggcattaa aaaattttat tattttatca attatctagg
    37021 aggaatataa ttttggagtg tcgaatatgc tttagagtag ataattagga agcaattgtg
    37081 taaaaagttt agttttctgt aataagaaga tgttttttaa tggggggatt tttagtggaa
    37141 aaaatcaaaa tagttttagc agatgacaat aaggattttt gtcaggtatt aaaagagtat
    37201 ttgtctaatg aagatgatat cgatatatta ggcatagcta aggatggaat tgaagcatta
    37261 gacttagtaa aaaagacaca accggactta ttaatactag atgtaataat gccacatcta
    37321 gatggattag gtgtaattga aaaattgaat actatggata tacctaaaat gccaaaaata
    37381 atagtactat cagcagtagg tcaagataag ataactcaaa gcgcaataaa tctaggagca
    37441 gattactaca tagtaaagcc atttgatttt gttgtgttta taaacagaat tagagagcta
    37501 gtttctaata gagttacaca agttgagcca aaacctagac cagttcaaga gactcaaatg
    37561 actagaagtg attttgttaa gaatgtagga aatatagaga cagaaatcac aaatataata
    37621 catgaaatag gagtaccagc tcatataaaa ggatatttat atttaagaga agcaataaaa
    37681 atggttattg ataatgttga gcttttaggt gcagtaacaa aagagttata tccaagtata
    37741 gctaaaaaat tcaatacaac accaagtaga gttgaaagag caataagaca tgcaatagag
    37801 gttgcatgga gtagaggaaa agttgacaca ataaatcaat tatttggata tacggtacac
    37861 aatactaaag gaaaaccaac taattcagaa tttatagcaa tgattgctga taaattaaga
    37921 ctagaacata gtatggttaa ataaacaaga cataaaaagt aaggcttttt aattaaggca
    37981 ttggctataa atgcgtatta caagcagcga aacggtataa ccactagggt tataccgttt
    38041 cgctatttta ataaatataa aaattttctt tattatttgc ttactatatc aatataataa
    38101 ctttattata ctatggatat agtatgtgtc tttacaagtt gtaaactgac agtggtttat
    38161 tttttaatat aaatattgac tttgatgcag gtaaactttg tatttttaag cgtattatgg
    38221 aatatgttaa ataaaaaaat gatgaaatat agtattgtaa atgccaaaga tgcaaaacaa
    38281 acttaaaaca tttattttat tgttaagtaa tgctataata taatgtgatt ttaataatga
    38341 tagtggaggt ttaaatatga gagtcgaggc ccctataaaa gtagatcgaa aaaccaaaaa
    38401 acttgctaaa agagttgaaa gtggggaaat agcagttata aatcatatag acatagatga
    38461 agttgctgca aactctttag tagaagctaa aataaaactt gtcataaatg cggctccttc
    38521 tataagtggt aggtatccca ataaaggtcc agggatatta actgaaaata atatactaat
    38581 aattgataat gttggagaag agttatttga agaattaaaa gaaggagaaa ctatagaagt
    38641 tgtagatgga aagatatata gaaaaggaaa atttttaggt gcaggagaag ttttagacaa
    38701 gtatgaggtt tcacaccaaa taaaggctgc ctatgagaat ttagctgtag aattggatag
    38761 atttatagat aatactatag attatgctaa aaaagaaaaa ggatttatac ttggagaagt
    38821 agaaattcca aaagtgaaaa ctaattatgc taataaacat gtattgatag ttgtaagagg
    38881 tcaggactac aaagaagatt taagtactat gctttcttat atagaagagg taaagccagt
    38941 attggttggt gttgatggag gagcagacgc tttaattgag tttggatata ctccagatgt
    39001 gattgtaggt gatatggata gtgtaagtga tgaagcttta aaaaaggcta gtgaaatagt
    39061 agtacatgct tatacagatg gaagagcacc aggtcttaaa agggttgagg aattaggctt
    39121 agatgcagtt gtatttcctg caccaggaac tagtgaagat atagctatgc ttatcgctta
    39181 tgagtataag gctgagttaa tagtagcagt tggtacacat tcaaatatga ttgattttct
    39241 tgaaaaagga agaaaaggta tggcaagtac ttttttagtt agactaaaaa taggttcaaa
    39301 gcttatagat gctaaaggcg ttaatttgtt atacagaagt aaattaaaaa taaagtacat
    39361 atgggcatta atagcaacag cattatttcc tgtgttggta gttgcatcat tgtcaccagg
    39421 agttcaacag ttcatacaac taatgcaatt aaagtttaga gtattattac agatgtaaat
    39481 tttaggagga taaatatgca tattaatatg aaatattata tagtcactat aggggctata
    39541 tttatagctt tgggtatagg catgttagta ggatttaatt taaataataa tcaacaatta
    39601 agtgaacaac aagcaaacat aataaatgat ttggatgata agtttgatat cttaaaagaa
    39661 aaaaatgata aacttgacag tgacttagca agtgtaaata gagattatga agaggcagtt
    39721 aattttataa ataaaaatgt agataagatt ttagctggtt cgctgaatgg taaaagtata
    39781 ggaataatat ctgctaatga aaatgacgat tatacaaaaa atatagaaga cataataaat
    39841 aaatctaatg gaagcattgc atttaatata atattaaaag aaaacataac aaatccagaa
    39901 aagttaaagg aaatgtcaac taaactaggt acagatgtaa aaaatgcaaa tgatgcagtt
    39961 aattatataa ttgacactct taaaaaggaa gatgcaagtg acattttaac atatcttcaa
    40021 gaactagatg taataaagtt taattttatt ggagatacat atctaaaata tgattccgtt
    40081 gtaatagctg gtggaaatga tgcaaaagac agtacaaaac agtttgaaaa aatagaaaaa
    40141 tttgtagttt caaaacttaa atcagagaat aaatatttag tagaagttca aaatacagga
    40201 gttaaaactt cttatgtaga actatattct aaaaacaaag ttgcaactat agacaatatt
    40261 gatgaaggta taggaagtat atctctagct attcttctac aacaaggtaa tatagttggt
    40321 aattttggta gattggatac tgcaacttca ttgttgccat ctattaaata gaaataattt
    40381 taaggagaga aaaaatggat tcttatataa gtataataat accagcttat aatgaagaaa
    40441 gtagaataaa atatacatta gaaagcatat tggacattaa gcaaataaat gaaataatag
    40501 ttgtggatga tggttcaatt gacaatactg ctaaggttgt atctgagcta gaaaatgaaa
    40561 agattaaatt ttttaagtta gataaaaata gaggtaaagg atatgcttta aattatggta
    40621 tcaaattagc aatgaaaaat gcagatatta taggttttct ggatggagat ttaggaagtt
    40681 ctgctaaaga agttgaaaaa ttaatagtac ctatattaaa tggtgaggcg gatgttacaa
    40741 tagccaagtt tcctcctgca aagagaaaag gaggtttggg atttgtaaaa ggtttagcta
    40801 aaaattctgt gtttgagatg actggtgttg agttagatgc aactttatca ggtcaaagaa
    40861 tatttaaaag agaagtttta gaaaagtttg atgaaatacc ttttggatat ggagtagaag
    40921 ttggaatgac cattgatata ttaaagtatg gatttaaaat taaagaagta ttagtaaata
    40981 tgactcataa tgaaacagga agaaatttaa aaggttttat tcatagaggg aaacagtatt
    41041 atcacataaa aaaggtttta ggacaaaaaa agaaagaatg gaggtaatat cttagtgaaa
    41101 gattatatac tttataccat tttattatta actggattat ttggaaccta tgctgtaatt
    41161 ccactattta aaaatttatt gataaatgga aatgtactta gaccaaacta taaaaaagat
    41221 atgataccag taagtatggg aattgtattt ttgcctatga ttgtaatcaa tggaataata
    41281 cttgctttct ttactacaga atttaaggat ttagcgtata tatttatgtt tatgtttggt
    41341 atgatatcca tgttttttgc agggatacta gatgatgtca ttggaaatag agatgtaagt
    41401 ggtttaaagg ggcattttaa aagtttattt aaaggaagtc ttacaacagg aggttttaaa
    41461 gctttatttg gtggatttgt agggcttata gtttctgttg ctatatcaaa agacataatt
    41521 gatataatag taaatacact aataatagct ctatcaacta atttaatgaa tcttttggat
    41581 ttaagaccag gaagggcaat aaaagcatat ttattcataa tggtaattat atttttaaca
    41641 ttgacaggat ttgttcaagt tcttccatta cttatagtac caaatgtact tgcatacttt
    41701 aattatgatt taaaagcaag ggctatgatg ggagatactg gttcaaacgt ccttggaata
    41761 tctattggaa tgctcatttc ttttgggtat ccatttaaca ttagattagg atggttaata
    41821 tttgtagtta ttatgcatat attcactgag aaatactcat taactaagat aatagaaaaa
    41881 aataaactat taaattttat agataaattg ggaaggtagt attatgataa gcaaaagagt
    41941 ggggattgtt gaatcaatag taagtcaaac tgaaacacta gatgatataa gagtaaatat
    42001 aaatggagaa atacaaaggg catataatta tcccaaaata tctggaacta taaatatagg
    42061 tgacgaagtg gttttaaata caacagcagt agaactgagc ttaggtacag gtggatacca
    42121 ttttgtaata actaatttaa ataatataga atctacttta actgaaggtg gacatataat
    42181 gaaacttaga tatacgcctc ttcaaataaa agtggattct gttgaggaac aagaaagtat
    42241 ttatcatgat aaatttgcga attttaaagg attagaaggt ctttctgttg tagtagggac
    42301 tcttcatagt atgttaactc catttgcagc ttcatttaaa agaaataatc caaataaaaa
    42361 attagtgtat ataatgactg atggagcatc tcttccaatt tatttaagta aaaatgtgga
    42421 tactttaaaa gaaaaaaaat taatagatag tacaataact ataggaaatg cgtttggtgg
    42481 agattatgaa tgtataaata tatatactgc attaattaca gcaaaagaaa tactaaaagc
    42541 ggatgcagta tttgtcagta tgggacctgg aattgctggg acaggaacaa aatatggatt
    42601 tacagggatt gaacaaggct ctattcttga tgctgtaaaa aaacttgaag gaagagcaat
    42661 tgcaattcca agaattagtt ttgctgacaa acgagaaaga catcaaggta tttcacatca
    42721 tagtatgact gtatttaaag aaattgtaaa tgtaaatgta gacataccaa taactatata
    42781 tgatgatgaa aaattgaact ttataaaaga gcaaatcaat ttaaatggtt tagacaaaaa
    42841 gcataatata atttatatag ataataataa aacgaaagag gatttagact attttaatct
    42901 aaaagtaaag agtatgggaa gaaattatga acaagatgag gcatttttca aagctgcaag
    42961 cacagcagca tattacttaa tggaggttta agatggtatt agaagaaaaa actattagta
    43021 gtgatagggt ttatacaggt aaagtaatta ctttaaaggt tgatactgtt gaaattccag
    43081 ggcaaggata tcaaaaaagg gagttagtag aagttggtgg agcagtagga atagttgcaa
    43141 tcacagatga taataaagtt gtgttagtta agcaatttag aaaacctata gaaaagccaa
    43201 tctttgaaat tccagctggt aaactagaaa aaaatgaaag tccaaaagaa tgtgctgaaa
    43261 gagaattaaa agaagaaaca gggtatagtg caaaaaatat taagctaata cataaatttt
    43321 tcacttcagc aggtttttct aatgagataa tgtttgtata tttagcaaca ggtcttactc
    43381 caggagagaa taatttagat gcagatgagt ttttagatgt ctatgaaatt gagcttgaag
    43441 aagcatataa catggtatta aaaaatgacg ttgaagatgc aaaaacatct attggattat
    43501 tattagtaaa agatatgttt aaaaattaat atttataaaa tgaataaccc tttgtcctaa
    43561 agcatattat tttataaagg acaaggggga attaaattga gaagaacata tagaaaaaat
    43621 gattttagag aattaaatag acaaataatt attataggat tgttgtttat gatgtctatt
    43681 gttgttgggt cttacataaa taaaatattg ccaggttcta gtaacaatat attaaacaat
    43741 ataaatcctg ctgtagagta ttataatcta aatataagca taaaagatac agtgatacaa
    43801 aatttaaaat cagatgcaat ctttatgggc tcaatagctc tattaagtct atttgtagta
    43861 acaattccag cagtgttggt agcttttgta ttaaaaggga tgtcaattgg ttatacaata
    43921 aatagttgta tattggcatt aaaatttaaa agtataaaaa tgattttaat aatattattt
    43981 aaaaatttga ttattattcc atgtgcaatc atattagcat taattagttt aagttatttt
    44041 aaagaaatgg tttatgaatt taaaaagaaa aatagaaaaa atatgcaatt tttaattaag
    44101 aggtatatac taaatattat aattataata gcactatcat tggggttgca attaatatta
    44161 aatactgcaa gtattggtat tattaagttc ctagctaaat aaaggagttt ataatggata
    44221 ttatagaggg atatatagat tacataaaag ataaaaaaaa attatcagag aatacagtgg
    44281 catcatactt tatggatata aaaaaatata tggaatatat aaatcaaaaa agcattaaac
    44341 tagtagatat agtagaaaat gatattataa gttacttaat aaagttggaa aaagataatg
    44401 tatcaatagc tacaatagcg aggatgatat cctctataaa atcttttcat gattatttat
    44461 ttttaaatca tatatgtaca aataatcctg caaaggatat aaaaaaacct aaaataaaaa
    44521 aagaaaatat aaatatactt acagaagaag aaattgaaag actattaaat tttccaaagt
    44581 taacaacacc taaattgatt agggacaagg ctatttttga agtattatat ggtactggta
    44641 taaaagtatc tgaattagta gagatgaata tagaggatat tgacctagat attgattata
    44701 tatattgtaa ttcatgttgt aaaaatcaaa gagtgatacc actttgtgat attacaaaac
    44761 tttacttaga aaagtatcta aaagaagcaa gacctaaaat ggctgtagaa ggtgaaaaat
    44821 cattattcgt aagttcatta ggtcaaaggt ttacacgaca aggtctttgg aaagtcataa
    44881 aaaaatattc taatctagcc aatatagata aaaatataaa tcctacaatg cttagacatt
    44941 catttgctat tcatttgtta aatgaaggtg ctaacattgc tgttgtaagt aaaattttag
    45001 gaaatgttaa tttatctagt ttgcaagtat atttaaatca tatagataaa aacgtgagaa
    45061 gagaaataaa ggagaaacat cctagaaatg atgttgatgt agaattacaa aaagcagaag
    45121 ctaaatagac tatatagcaa attttataat ttagaaggag catatttata tgagcagagt
    45181 aatttggatt gtaattgata gtgttgggat aggagcattg cctgatgctg aaaaatttgg
    45241 tgatagtaag gatgtaagta cccttggaaa tatatttaag gaatatccag atattcagat
    45301 tcctaatatg agaaatttag gtataggaaa catagatggt atagattttt ttgaaagtat
    45361 aaaagaacca ataggttgtt ttggaaagtg taaggaaatg tctcagggaa aagatactac
    45421 tacaggtcat tgggaaatga ctggtataat agttgacaaa ccatttaaga catttgaaca
    45481 tggattctct aaggagataa tagaagagtt tgagaaaaaa actggaagaa aagtagtagg
    45541 aaataaacca gcttcaggaa ctgtaataat agatgagtat ggagaacatc aaataaaaac
    45601 tggagatgta atagtatata catcagctga tagtgtattt cagattgctg ctaatgaaga
    45661 agtaattccc ctagaagaac tctataatat gtgcaaaatc gctagggaaa taatgatggg
    45721 ggataatgca gtagctagag taattgctag accattcata ggtaagaaaa aaggtgagtt
    45781 tgtgaggact tcaaatagaa gagattattc actagaccca tttgaaccaa cagttcttga
    45841 taatataaaa gaatcaggac ttgatgtttt agcagttgga aaaatagaag atatttttaa
    45901 tggaaaagga attacagatg ctatccacac taaaagcaat atggatggag tggatgaaac
    45961 tttaaattat atgaaacaag ataataaagg tctaatatat tcaaatctcg ttgattttga
    46021 ttctaaatat ggtcatagga gagacccaga aggatataag aaagctcttg aagagtttga
    46081 tagtagactt cctgaaataa tggctaatat gagagaagat gatattttaa taatcaatgc
    46141 agaccatgga aatgacccaa catataaagg tacagaccat acaagagaat atatacctgt
    46201 tatgatttat ggcaataaga ttaaaaaagg ttttaattta ggagtaaaag atacttttgc
    46261 tgatattgga gcaactgttg cagatatact aaatgtaaag ttgcctaaac atgggtcaag
    46321 ctttaaagaa gatttatttt aaattataaa atgataaaaa cttagcttaa taggatggag
    46381 agattggaat gtttgaaaag atagcacaaa gtagtaaatt tataaattct aaaagtaata
    46441 taaaacctaa gattggtttg atattaggtt ctggactagg tgatttagca aatgatatag
    46501 aagaccctgt cacaattaaa tatagtgaaa ttccaaactt ccctgtatca acagtagcag
    46561 gacatgctgg acaacttgta attggaaaac ttgaaggaaa agaagttatt gctatgcaag
    46621 gtagatttca ttactatgaa ggatattctc agaaagaagc tacttttcca gtaagggtta
    46681 tgaaagaatt aggagtcgag attcttatag taactaacgc tgctggaggc gtaaataaag
    46741 aatttaaagc aggagatttg atgcttataa gagaccatat aaactttagt ggaagtaatc
    46801 cattagtagg aaaaaatgat gacagatttg gtgctagatt tccagatatg tcagatgcat
    46861 attcttctaa atatgtagat gtagttaaaa actgcgctaa agaatgtaat atagatgtca
    46921 aagaaggcgt ttatatgttt tttagtggtc caaactatga aacaccagca gaagtgagaa
    46981 tggcacaaat tttgggagct gatgctgtag gtatgtctac tgttccagaa gtcatagtag
    47041 cttctcactc taatatagga gttattggta tatcttgtat aacaaatatg gctgctggca
    47101 tcttaaatca accactaagt catgaggaag taatagaaac tactcaaaaa gttaaatcag
    47161 aatttatgaa tttagttaaa acaacggtta aaaatttata agttaaagct tattatagat
    47221 tttttgagga agtttataat aaaatagtta atgtttaatt tgaaaagaat taataaaaag
    47281 ctgtcttgat atagaaagtt atttctaata aaggacagtt tttttataat actacaacat
    47341 ttgaaagtgt tttaaagttt aatatttatt taaattatta taggttagaa tggagatttc
    47401 aattatgaga atgtatgata ttataaaaaa aaagagagat aacctagagc ttagtaaaaa
    47461 agagataaac tactttatag aaaactattc aaagggagat atacctgact atcaagcttc
    47521 tgcactatta atgtcgattt atttaaataa gatgaataaa caggaaactg tacatcttac
    47581 agaagcaatg atgaactctg gagacatgat aaacctatct gagatagatg gaataaaggt
    47641 agataagcat agtactggtg gtgttggaga taaaactacc atagctctta tacctttggt
    47701 tgcttcatgt ggagcaccag ttgcaaagat gtcaggtaga ggtcttggtc atactggtgg
    47761 tactattgat aaactggagt ctataccagg tttttcgaca gaaatggaga taagtaaatt
    47821 tattgattct gtaaataagt ctaaaatagc agtttgtggt caaagtgcaa aggtagcaat
    47881 tgcagataag aaaatatatg ctcttagaga tgttacagca actgttgata atatatcatt
    47941 gatatcatca agtgtaatgt ctaaaaagtt agcttcagga gctgatgcaa ttgtgctaga
    48001 tgtaaaaact ggtgatggag catttatgaa gacattagaa gagtcttttg agttagctaa
    48061 atctatggtt gatataggta ctggtatgaa tagagataca atagggattg taacagatat
    48121 ggatgaacct ttaggttttg cagtgggaaa ctctttagaa gttatagagg caattgagac
    48181 attaaaagga aatggaccta aagaccttgt tgagctatgc gaagtattag gagcatatat
    48241 gttggtttta tcaaaggtag cacatgattt tgaagatggt gttgaaaaaa taagagaatc
    48301 aataaaatct ggtactgcta tacaaaaact caaagagttt atagagaatc aaggtggtaa
    48361 taaagatgta gtagatgatt attctttatt caaaaaatca aagtataaat atccagttaa
    48421 atcaactaaa tcaggttata taagcaaaat taaagctgaa gatgttggtg tatcggctat
    48481 gattcttgga gcaggtagag aaactaaaga tgatatttta gatttatcag cagggataat
    48541 tttagaaaag aaagtaggag attatgtaaa tgaaggtgaa gttttagctt acatgtatta
    48601 taatgatgaa caaaagcttt cattggcaaa gggaagattt attgattcct actctattgt
    48661 agattcaaaa atagaaaaaa ataaattaat atatggtatt gtaactaaag aagagattaa
    48721 aaagttttag aaaattttag aaaagtgatt ataaaaggcc taacatgtca aaaataatgt
    48781 taggtttttt attttttgat agactataaa ttataccaaa aatatatatt ttaa
//
LOCUS       DAGSWI010000010        47338 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.010, whole genome shotgun sequence.
ACCESSION   DAGSWI010000010 DAGSWI010000000
VERSION     DAGSWI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.010"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(139..226)
                     /locus_tag="MJF80_000522"
     tRNA            complement(139..226)
                     /locus_tag="MJF80_000522"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(190..192),aa:Leu,seq:caa)
     gene            378..3026
                     /gene="polA"
                     /locus_tag="MJF80_000523"
     CDS             378..3026
                     /gene="polA"
                     /locus_tag="MJF80_000523"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBZ0279201.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            3035..3637
                     /locus_tag="MJF80_000524"
     CDS             3035..3637
                     /locus_tag="MJF80_000524"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBZ0279202.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            3630..4184
                     /locus_tag="MJF80_000525"
     CDS             3630..4184
                     /locus_tag="MJF80_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBZ0279203.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            4184..5899
                     /locus_tag="MJF80_000526"
     CDS             4184..5899
                     /locus_tag="MJF80_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBZ0279204.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            5972..6184
                     /locus_tag="MJF80_000527"
     CDS             5972..6184
                     /locus_tag="MJF80_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBZ0279205.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            6385..6984
                     /locus_tag="MJF80_000528"
     CDS             6385..6984
                     /locus_tag="MJF80_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279206.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKQLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            7212..7583
                     /locus_tag="MJF80_000529"
     CDS             7212..7583
                     /locus_tag="MJF80_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBZ0279207.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            7715..8989
                     /locus_tag="MJF80_000530"
     CDS             7715..8989
                     /locus_tag="MJF80_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBZ0279208.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEGTKEKPNSSSN
                     QNSQPNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            9100..10044
                     /locus_tag="MJF80_000531"
     CDS             9100..10044
                     /locus_tag="MJF80_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279209.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            10260..11597
                     /gene="rsxC"
                     /locus_tag="MJF80_000532"
     CDS             10260..11597
                     /gene="rsxC"
                     /locus_tag="MJF80_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBZ0279210.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            11620..12597
                     /locus_tag="MJF80_000533"
     CDS             11620..12597
                     /locus_tag="MJF80_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBZ0279211.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            12597..13166
                     /locus_tag="MJF80_000534"
     CDS             12597..13166
                     /locus_tag="MJF80_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBZ0279212.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDDLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            13179..13772
                     /locus_tag="MJF80_000535"
     CDS             13179..13772
                     /locus_tag="MJF80_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBZ0279213.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            13787..14362
                     /gene="rsxA"
                     /locus_tag="MJF80_000536"
     CDS             13787..14362
                     /gene="rsxA"
                     /locus_tag="MJF80_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBZ0279214.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            14378..15349
                     /locus_tag="MJF80_000537"
     CDS             14378..15349
                     /locus_tag="MJF80_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBZ0279215.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            15569..16153
                     /gene="maf"
                     /locus_tag="MJF80_000538"
     CDS             15569..16153
                     /gene="maf"
                     /locus_tag="MJF80_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBZ0279216.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            16198..16857
                     /gene="radC"
                     /locus_tag="MJF80_000539"
     CDS             16198..16857
                     /gene="radC"
                     /locus_tag="MJF80_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBZ0279217.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            16978..17988
                     /locus_tag="MJF80_000540"
     CDS             16978..17988
                     /locus_tag="MJF80_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBZ0279218.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            17998..18891
                     /gene="mreC"
                     /locus_tag="MJF80_000541"
     CDS             17998..18891
                     /gene="mreC"
                     /locus_tag="MJF80_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBZ0279219.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            18906..19394
                     /gene="mreD"
                     /locus_tag="MJF80_000542"
     CDS             18906..19394
                     /gene="mreD"
                     /locus_tag="MJF80_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBZ0279220.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            19406..22384
                     /locus_tag="MJF80_000543"
     CDS             19406..22384
                     /locus_tag="MJF80_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBZ0279221.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            22482..23165
                     /locus_tag="MJF80_000544"
     CDS             22482..23165
                     /locus_tag="MJF80_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBZ0279222.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            23189..23986
                     /gene="minD"
                     /locus_tag="MJF80_000545"
     CDS             23189..23986
                     /gene="minD"
                     /locus_tag="MJF80_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBZ0279223.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            24004..24288
                     /gene="minE"
                     /locus_tag="MJF80_000546"
     CDS             24004..24288
                     /gene="minE"
                     /locus_tag="MJF80_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBZ0279224.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            24373..25503
                     /gene="rodA"
                     /locus_tag="MJF80_000547"
     CDS             24373..25503
                     /gene="rodA"
                     /locus_tag="MJF80_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBZ0279225.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            25544..25957
                     /locus_tag="MJF80_000548"
     CDS             25544..25957
                     /locus_tag="MJF80_000548"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBZ0279226.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            26442..26771
                     /locus_tag="MJF80_000549"
     CDS             26442..26771
                     /locus_tag="MJF80_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0279227.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            26775..27566
                     /locus_tag="MJF80_000550"
     CDS             26775..27566
                     /locus_tag="MJF80_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279228.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            28130..29212
                     /locus_tag="MJF80_000551"
     CDS             28130..29212
                     /locus_tag="MJF80_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBZ0279229.1"
                     /translation="MKKIRKLATKLLIATMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(29424..30617)
                     /locus_tag="MJF80_000552"
     CDS             complement(29424..30617)
                     /locus_tag="MJF80_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBZ0279230.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            30936..32780
                     /locus_tag="MJF80_000553"
     CDS             30936..32780
                     /locus_tag="MJF80_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBZ0279231.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            32782..33483
                     /locus_tag="MJF80_000554"
     CDS             32782..33483
                     /locus_tag="MJF80_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBZ0279232.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            33501..34922
                     /locus_tag="MJF80_000555"
     CDS             33501..34922
                     /locus_tag="MJF80_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBZ0279233.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            35023..35334
                     /gene="rplU"
                     /locus_tag="MJF80_000556"
     CDS             35023..35334
                     /gene="rplU"
                     /locus_tag="MJF80_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBZ0279234.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            35349..35666
                     /locus_tag="MJF80_000557"
     CDS             35349..35666
                     /locus_tag="MJF80_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBZ0279235.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            35680..35970
                     /gene="rpmA"
                     /locus_tag="MJF80_000558"
     CDS             35680..35970
                     /gene="rpmA"
                     /locus_tag="MJF80_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBZ0279236.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            36081..37358
                     /gene="obgE"
                     /locus_tag="MJF80_000559"
     CDS             36081..37358
                     /gene="obgE"
                     /locus_tag="MJF80_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBZ0279237.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            37410..37778
                     /locus_tag="MJF80_000560"
     CDS             37410..37778
                     /locus_tag="MJF80_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBZ0279238.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     regulatory      37870..37976
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            38079..39284
                     /locus_tag="MJF80_000561"
     CDS             38079..39284
                     /locus_tag="MJF80_000561"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBZ0279239.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SVKKLLKID"
     gene            complement(39485..40051)
                     /locus_tag="MJF80_000562"
     CDS             complement(39485..40051)
                     /locus_tag="MJF80_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBZ0279240.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(40331..41590)
                     /locus_tag="MJF80_000563"
     CDS             complement(40331..41590)
                     /locus_tag="MJF80_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBZ0279241.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            41715..42428
                     /locus_tag="MJF80_000564"
     CDS             41715..42428
                     /locus_tag="MJF80_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBZ0279242.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            42546..43820
                     /gene="larA"
                     /locus_tag="MJF80_000565"
     CDS             42546..43820
                     /gene="larA"
                     /locus_tag="MJF80_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBZ0279243.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            43837..44625
                     /locus_tag="MJF80_000566"
     CDS             43837..44625
                     /locus_tag="MJF80_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBZ0279244.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKNENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            44639..45859
                     /locus_tag="MJF80_000567"
     CDS             44639..45859
                     /locus_tag="MJF80_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBZ0279245.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            45870..47261
                     /locus_tag="MJF80_000568"
     CDS             45870..47261
                     /locus_tag="MJF80_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBZ0279246.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
BASE COUNT        18693 a         5216 c         8920 g        14509 t
ORIGIN      
        1 aaattaatgc tagaagataa atttatataa ttatataaat tttaaaaaga cacagtaatt
       61 tcaaaaaata acttaaaatt taaatcaaaa ataaaaaaac tcctaatcta ttgaataaat
      121 agctttagga gtttttaatg gtgcccgagg ccggactcga accggcacgg agtattagtc
      181 cggtggattt tgagtccact gcgtctacca attccaccac tcgggcatgt catgtatatt
      241 attatagcaa aaaaatatat tgtttgcaac atatttaatt ttaatatact attattatta
      301 tgggaatatt aaataacagt atattggtat ataatatata tgttacaaaa atagataatt
      361 tttaggagtg atataatttg gaaaaaaaat taattataat agatggaaat tcaataataa
      421 atagagcttt ttatgctctt ccagaaatga ataataaaga agggctaaag accaatgcaa
      481 tatatggatt tacaacaatg ctatttaaaa tgatagatat atataaacca acacatataa
      541 gtgtagcatt tgacagaaaa gcacctacat ttagacattt agaatataaa gagtataagg
      601 ctggaagaaa gggaatgcca gatgaactgg cagaacaatt acagccatta aaagaccttt
      661 tagataaatt caaaataaat agattggaaa tagatggcta tgaggcagac gacattatag
      721 gaactgtttc taaaaaagca gaagaaaatg gatataaggt atacatagta actggagata
      781 aagatgctat tcaacttgct tcagataata caactaccct tataacgaaa aaaggggttg
      841 gagaagtaga agaatacaac tttaattctg taattgaaaa atatgagatg actccaacac
      901 aatttatcga tttaaagggt cttatgggag acaagtctga taatatacct ggagtacctg
      961 gaataggtga aaaaacaggt ataaagctta taaaagagtt ttctagtata gagaatctaa
     1021 tagagaatac agataagctt aaaggtagtg taaaaaagaa aatagaggaa aacaaagaaa
     1081 tagctattca aagtaaaaga cttgcaacaa ttataagaga tgtgccaata gaagtggatt
     1141 tagatagtat gatttttggc gattatgata aagatgaact tttagataaa tttagatact
     1201 ttggatttac aagtctctta tcaagattag ttgatttggt cgattcagat gacacagaac
     1261 acgtagaaga aaaaatagaa atattaaaat taaaagatat agaaaaattt atagatgaag
     1321 taaacaaaaa aggacaagtt attttaaaaa ctgttagagg acaaggtaat atacttgaaa
     1381 aaaatataat atatattttt ataagtgtag atggagaaaa aatatattat gtagattcaa
     1441 atgaagtgtc aaagttagat agtatatttt caaataatga aattaaaaag tatggatata
     1501 atttaaaaga tgattatata tctttaaaac catataatat aaacttagaa aatatgtatt
     1561 ttgatataac aatagctgag tacttaatag attcaacttc atctacctca tatgattgta
     1621 gtgcaatagc tatgaagtat ttatcaaaaa aggttaaatc aaaagaagaa ttattaggaa
     1681 agggtgttaa atctaaaaac tatgaggatt tagaatttga ggatttagca gagtatatgg
     1741 gtcaaataat atgcacagtt aaagaaacag ttcctatgat ggaaagaagt ctaatggata
     1801 tgagtatgga tggtcttttt tatcatgtag agatgccttt agtagaagtc ttaggtcata
     1861 tggagtatga aggtataaaa gtagacaaaa gtatattgga tgaattgagt gtagagttta
     1921 aagaaataat agctacatta gaaaaggaaa tttatgaatt atcaggagag ccattcaata
     1981 taaattcacc aaaacaattg ggtgttatat tatttgaaaa attagagctt ccaataatta
     2041 aaaaaactaa aactggatat tcaactaatg cagatgtttt agaaaaatta agagataagc
     2101 atccaataat agataagata actgagtata gacaaatagt aaagttaaat tctacttacg
     2161 ttgaaggact tttaaatata ataaatccag aaagtaatag aatacattca tcatttaatc
     2221 aaactataac gactacagga agaatttcat ctacagaacc aaatcttcaa aatataccta
     2281 taaaaatgga aatgggtaga aaaataagaa aagtgtttat tgcagatgat aactgtaagt
     2341 tggttgatgc cgattactca caagtagagc taagagttct tgcacatatg agtcaggatg
     2401 aaaatatgat agaagcattt aaacatcatg aagatattca taccaaaaca gcttcacaag
     2461 tttttaatgt tcctatggaa gatgttacta gtgaattgag aggtgcagct aaagctgtaa
     2521 actttggtat tatatatggt aaaagtgact ttggtctagc tgatgattta aatatacctg
     2581 ttccaaaggc gaaagaatat atcgaaagtt attttgctaa ataccataaa ataaaagagt
     2641 ttatggacac aacagtagaa aaggctagtg aggatggata tgctgtaaca attttaaaca
     2701 gaagaagata tataccagaa ataaagtcaa gcaattttat ggaaagaaat agaggaaaga
     2761 gatttgcaat gaatgcacca atacaaggaa gtgcagcaga tataataaaa attgctatga
     2821 taaatgttca taaaaaattg gaagagaaca atttgaaatc aaaacttata ctacaagttc
     2881 acgacgagtt gatagttgaa gcaattgatg atgaaataga tatagttaag aaaatagtaa
     2941 aagaagaaat ggaaaatgct gtaaatatgg atgttcactt agatgtagat ttaaatgtag
     3001 gtagttcttg gtatgagaca aagtaggtga ttttatgttg atattaggac ttacaggagg
     3061 aataggctgt ggaaaaagca gcctatcaaa tatatttaga aatcttaaca ttcctatagt
     3121 ggatgctgat attatatcca ggaagatatt tgaggataag ttgttattgg aaaaagtatt
     3181 tgtccatttt gggcaaagta taaaaaatga tgatggaact ctaaatagaa aagctcttgg
     3241 gaaaatagtt tttagtgatg aagaaaaact taaggaatta aataacttga ctcatccacg
     3301 aataagagaa aaaataataa gtgaaataga aaaattgaga aaaaaaggcg aaaatatagt
     3361 agtgttagat gcggcaatat tggtagaaag tggtttttta gatatggttg acaagctatt
     3421 agtagttact tgtaaacaag aagtacaaat tagtagaata caaaagagag ataattgcag
     3481 tgagcaggaa gcacttagta ggataaattc acagatgagt caggaagaaa aatccaaata
     3541 tggtgattat ataatagata attcgggaac aataactgaa ttagaaagta aagcacataa
     3601 atttattgag tacatgaagg agaattggcg tgaatagtaa gaaagtattg attctatcta
     3661 tttttataat cttatttggg gcactattaa tggaaagcaa agtaatacat aaatttttat
     3721 atcctaaaaa atattcagag tatgtagaaa agtattcgaa agaatttaat ttagatgaaa
     3781 atatagttta cagtgttatt aaagccgaaa gtaagtttaa tagttctgct gtttcaaaaa
     3841 aggaagcaaa aggattaatg caaatattag acataactag agattgggga gcagaggaac
     3901 taaatttaaa aaatgtggat attttcgacc cagagactaa tataagactt ggctgttggt
     3961 atttaagtaa gttatacaaa gaatttggta aattagattt agtgatagct gcatataatg
     4021 gtggttcagg taatgtgaaa aaatggttag aaaataatga atatagtaaa gatggcgaaa
     4081 atctacatga tatacctttt aagcaaactt caaaatatgt agaaaaagta aaaaataatt
     4141 acgaacatta taataagata tatggcaaga aaggaaaaaa ctaatgaaga gaataaaagt
     4201 cttaacagtc gttttagcta taactcttat ggtagctggt tgtagtaata ctaaaacaaa
     4261 acaaagtagt agtgattcaa gtttacaaag tcaaagttct atagatgcta agtatataaa
     4321 cttaacaatg gtaactccaa aaactataaa tcctataact aatactgata agtctgttgg
     4381 ttatataatg aatttagttt atgatagttt atttactata gatgagaact acaatgtaat
     4441 tcctcaatta gttaaggagt ataatatagc tcaggatgga atgagtatag atataaagct
     4501 aaaagatgca aagtggcatg atggaaaaaa tgttacttca aatgatgtaa actatactat
     4561 tggtttaatt caaaaaagtg cagatagtcc atataatgaa tttactaaaa atatagcttc
     4621 tgtgaatatt aaatcagata aagattttac aataaaattt aaagctagat atgctttttc
     4681 aattgatagt ttaatttttc caattgtatc tcaaaatcat ttagattcaa aagatgtaaa
     4741 tgacaagaat aataatatga taggaaatgg gaaatataaa atagaatcat atacagaaag
     4801 agaaggaatg gttttatctg taaacaaaga ttactatgaa gaagttccaa aaaccatgaa
     4861 gaacataaaa gtaggtatgg tgcctaatga agatgcaagg acatctatgg ttatggctct
     4921 tgatagtgat ataaccaatg ttacattaaa tgatttaagt aagtttcaag aaaaagagtt
     4981 taatataaca aaatatcaag gaagagatta tgagtgtgta ttatttaatt ataataatcc
     5041 ttttttcaaa gatgttaatt ttagaaaagc aattgcacac tctatagata aagatagaat
     5101 tattagtgaa ggttatatgg atgatgctac tccagttaat tttccactta attcaaaatc
     5161 aaaatactat aatagtgaaa tgaaggattt agagtttaat aaagataagg cgatagagtg
     5221 tcttgctaag gtagaatatg caaatgtaaa ctcagtaaat caaaatgaca ataaagtaaa
     5281 aaatcagaaa aaatcagcta aaaagaactc aaagaagctt acgccagaag aagaagcaaa
     5341 agctaaaaaa gcagaagaag atagaatcaa aaaagaagct gaagagaaaa aaaatagaga
     5401 aaaagaggaa gtaaagaaaa gtctttctga aatgaactta aagataatag taaacagaga
     5461 taatagtgag agaataaaaa ctgcaaacat aataaatgaa aacttaaaag ctataggaat
     5521 aaatagtaca gtaaatcagc tgtcagataa ggatatggaa aatgctctta attctaaaaa
     5581 ttatgattta gcattagttg gatggaaact atcaattata ccagatgcaa gtagtattat
     5641 agcgagtagt ggatatactg atgataagtt aaatggatat atgtctgcac ttacaagttc
     5701 tacgtctgaa attgaaacaa aaaaagcata caaagatgta cagacatata taaaagataa
     5761 tgcaacattt ataagtttag ctgtgagaaa caactatatt gtaagtaata gtagattaaa
     5821 aggtaaaatt actccaaatg attttgatgt atatgaggga atatctaatt tagatataaa
     5881 atcgaatgaa tcaaattaaa tgattaattt ctatttaaaa agtataaact atatgataga
     5941 atacccttat agggtataag gaggtaatta aatgaagaag aaactattag tagaaggtat
     6001 gagttgtggt cattgtgtaa accatcttaa aactgcatta acagaagata tagatggtat
     6061 tgaagtttta gatgtagatt tagaaaataa gtgtgcatct gtagatatga aagatgatgt
     6121 atctattgaa aaattaaaag aagtgatagc agaattagga tttgaactaa agggaattga
     6181 atagaaattg ataaactaaa aaaagacctc tattggggga gtaatagagg tctaaaaatg
     6241 ggggagattg agtatgtttg attttacatt actattatct ccccttttaa attaatatat
     6301 acataaaaag taaaaaaatt ttgtaatgac tcacatttct tgagcttttt gaatatacta
     6361 taaatagcta agggagggag tatcatggaa tctaggggat ataaagagga tttaaaaaaa
     6421 atgatacttg atatggccca caaagaactt gagagttatg tatcacaaaa aggagcaaaa
     6481 aaatactttg aaggtaactt ggataagatt attaataata atataaaaaa attcaatcaa
     6541 agtattggtc ttaatagagt atatttaaaa atgttaaaaa agtgtgcata tcaagaaagt
     6601 gtaacttcaa tatcaagaac tataaagaaa gaagcattat ttaaatcaaa aaaagagcta
     6661 tttgcttttt cagagtactt aaaacttgat attaacaagc aactttcata taatcaaatt
     6721 ttaagaaaaa tatcaagata tatatactac aatagaagtt catatgctca aaaatacgtt
     6781 gttttcaaaa gaggagatga agaatatctt ttagaaccag agaaaataaa agatgaatta
     6841 atatcaagct ataggtcaaa gactagagaa gatatgaggt caatagcaaa acttttggat
     6901 atagaagtag aagatgatta taatgctgaa gatattagaa aaaaagtaat aaattatatt
     6961 attaaagaaa aacttaaaag ttagagtttt aaagaataag ttctgccaat ttgcatgatt
     7021 aagtataact tgggtataaa ttttttatgc atttgaaaga gcttattctt tttttgacat
     7081 attcttttaa ataatttacc atgatttggt aattggtcat catcattcaa atttttaact
     7141 taatatgatt taaataaatt tataataatg atataatata aaaaaaataa aaattggaga
     7201 gtgatttcaa aatgaagttt aatttttgtc ataacaattt caatgttact aatttagaaa
     7261 aaagtttaga tttttataaa aaagctttag ggcttaaaga ggtaaagcgc aaggaagcag
     7321 aagatggaag ttttatatta gtttatttag gagatggcat tactagtcat acattagaat
     7381 taacttggct tagagattgg gatagaccat ataatttagg agacaatgaa tttcatctgg
     7441 ctttagaagt tgaggagttt gatgaggcaa aaaaacttca taaagattta gattgcatat
     7501 gttttgaaaa tgaaagtatg ggaatttatt ttatagcgga cccagataat tattggattg
     7561 aaatacttcc taaaaatcac taattttaag gatatgttta taatttcttt acaattaata
     7621 agaaaataag ttatcattaa attgtaggga ctaaaaatgg ataataatgt tatgaattta
     7681 tgactataaa aatttgttgg ggggagtgat agtagtgaaa aaagctatag cagctttagg
     7741 aattggggca gtagcagtat ctgttagctc cataaatgca agtgctcttg aaaagggaac
     7801 agtaacagca agtgccttga atataagaag tggaccaagt tctgattgcg ataaggtagc
     7861 aaaattatac aagggaaaaa ctgtagagat tttagaaaag tctaatggat ggtataaagt
     7921 aagggtatcg agttctgttg ttggatgggg aagtgcaaaa tacatatcaa caagtggttc
     7981 gtctgaaggt acatcaagtc aaaataatcc aacttcaagt gggacaacta ttagtggaaa
     8041 tggtaaagta aatgtgagtt ctagactgaa tgtaagaagt ggtgcaggta ctaactattc
     8101 attagttggt aaagcaaata atggagatgt agtaaagtta ctagaacaaa gcaatggatg
     8161 gtataaaata aaactatcaa atggagttac tggatgggct agtagccaat atatttctaa
     8221 aacatcagaa gatgttggaa cgaacaattc tagtaattca aatagtacta ataattctga
     8281 taaaaaacca tctagcgaag aatcaattga aggcaagaat ggtaaggtaa catctgctgt
     8341 gagtttaaat gtaagaagtg gtcctgggac aagctattct ataataggaa aattaaatgg
     8401 tggagatgtt gttgaattaa aagctaaaag taatggttgg tataaggtaa agttatcgag
     8461 tggaacaatt ggatgggtga gtgcaagtta tatttctgag actaatgaag gtacaaaaga
     8521 aaaaccaaat tcatcatcta accaaaattc tcaacctaat agtaactcaa agccatcatt
     8581 tactggaaat tcagataaat ctacagcaaa aggttcaaca atcgtagatt ttgcatatac
     8641 tcttattgga ataccatatc aatggggagc ttcagggcct gataaatttg actgttctgg
     8701 atttacacag tatgttttta aacattctgt aggcgtttct attcctagag tttctagaga
     8761 acaggctaat tttggaagtg caatttctat ggggaattat gcaccaggag atttagttta
     8821 ttttgacact gatggtgatg gaacaactaa tcatgtaggt atatatgtag gaaatagtaa
     8881 atttatccat tgtagtggta ctcagactaa tcctaataag gttaaagtgg ataatttaac
     8941 tagttcatat tggtctaaag tattacttgg tgcaagaaga tttgtttagt aagtacaatt
     9001 tcttattaaa aaattagaag aaataattat ttatattcat aaattgtgac tcttaaaaat
     9061 atactttatt aataatataa ttttgggagg gacaaactaa tgaatagaag aaaaacgata
     9121 tacagaggat ttaataataa tagacataag attgatttac gtaaatatgt tataacgatt
     9181 gcttgcttgt gtctaattgg ctattattct tatacaaaaa taaaagatag caagatattg
     9241 gagtatgtat cggctaaaat accatttcta aataattcat cagatataac ttataaagat
     9301 atatctgatg aattaaattc aataaaaaaa gggaaaaaat ctaaatcaca aaccaattca
     9361 gatgataaac aagaaactaa tccagaaaaa gcggtaaata acactaaaga gccagaagaa
     9421 gtcaaattag ctactataga aggctgggat atgtatacta ttcaagttgc tgcaatagat
     9481 aataatgatg atttgaaaaa aatacagaca tcattagtta ataatgacat acctttttca
     9541 gttatggaga aggatggagt aaaaaaaata cagacctatt cctcttttga tgaaaatgat
     9601 gttagaaagc agataagcag tgtaagaaag gtgtttccag atgcattttt atcacactta
     9661 gatgcaccta tgttgtcttt agagtataca agtaactacg cttatataga gagtatatct
     9721 aaagaattaa ataaattaat aacaaacttt aaagaagaat caagtttttg gtcgaatgct
     9781 gaaaataatg tagatatgga aaaatataat actattttga caaatagaaa ggcaatatca
     9841 caaaatatat caaaagaagc tgaaaagatt gattattctg aaatgcgttt atttaaagat
     9901 aatttaatag agtatgtaaa gaatgtgaat gaaaaaattg atacagcttc aaaagcagca
     9961 aatgaagaaa aatatagtgt aagtaaaagt ctgcttttat catccatgca ggagtactct
    10021 atgtttataa attctataaa ataaaataca gaaaatttgg actttagaaa gctatatgaa
    10081 aaggattgtt taattaagca atccttttcc tcaatttgac ttaatataga ggtgttgatt
    10141 tataaaataa attataaaaa tacggatatt tttttaacaa ttgttaagat tttttaatat
    10201 atatagtata cttatagaag gcagaaaaaa tgttttttta tagataaggg ggtaaaacaa
    10261 tgaaactctt aacttttaag ggagggatac atcctccata tagaaaagag tactctaaca
    10321 caaaggcact tgaaaaagct caagctccaa agatagttta cattcctctt cagcaacata
    10381 taggggcacc agctaaacct attgttgaag ttggtgatga agtaaagttt gggcaaaaaa
    10441 taggtgaaca acaaggtttt gtttcatgta acgttcattc ttcagtatca ggtaaagtta
    10501 ttgctattga acaacatgaa gtaccaggag gttcagctca atgtgtagtt atcgaaaatg
    10561 actttaagga agaattacat gagagtgttc aaccaaaagg tcagttagag gatttgagca
    10621 aagaagatat agtaggtatc ataaaagagg caggaatagt aggtatgggt ggagctacat
    10681 tccctaacca tgtaaaagta tctcctccac cagatagcaa ggcagaagta gttatattaa
    10741 atggtgcaga atgtgaacca tacttaactg cagaccacag attaatggtt gaaaatccag
    10801 aagatgttgt gtttggttta agagcattaa tgaaggtatt agatgtcaaa aaaggattta
    10861 taggaattga aacgaataaa ccagatgcta tagaagcaat tcaaaatgta gctaaagact
    10921 atagcgaaat cgaagttgtt ggccttcaag tgaaatatcc acaaggagca gagaaacaac
    10981 ttatttatgc gtgtacaggc aaagaagttc catctggtgg attaccaata gcagcaggtg
    11041 cagttgttga caacgttgcc acagcagcac agatagctaa gtctattaaa acaggtatgc
    11101 ctttggttga aagaatcact acaataacag gtagttgcat caaagagcca aagaacctaa
    11161 taacaaaagt tggaacatta gtttcagaaa taatagaaca atgtggtgga tttaaagaag
    11221 ataagaagat tggtaaggtt ataatgggtg gacctatgat gggaatagct caatatacta
    11281 ctgaaatagc gactaacaaa ggttcttcag gaattttatg tttggatgag gaagaatcac
    11341 gtactccaga tattcaaaac tgtttaagat gtggaagatg tacagatgta tgtccatcat
    11401 tcttacaacc acttttcata agtgcatatt ctttaaaaga tgactatgac acagctgaat
    11461 atcatagagc aatggattgt atagaatgtg ggtcttgctc atttatttgt ccagcaagaa
    11521 gaccattact tcaatctata agatcagcaa agagagaaat aggagcaaag agaagaaagc
    11581 aagctgctca gaaataaaga aagttaaggg ggaaaaacga tggaaaataa gttgatagta
    11641 tcatcttctc ctcatgtgag aagtaatgaa gatacttcat atataatgaa acaagttatt
    11701 atagcactcc ttccagcagc agtagcagga gtatacttct ttagacttaa tgctttgagt
    11761 gctatgtttt tttgtatact tggtacagta ggtactgaat ttttatatca aaaatttacg
    11821 aagcaaaaaa gtactatagg agatttttca gctgttgtaa caggattatt attagcattt
    11881 aacgtaccag catcacttcc ttggtggatg tgtctagtag gaggaatatt tgcaatatta
    11941 gtagttaaaa tggtatttgg tggaattgga tgtaactttg tcaatccagc acttgctgca
    12001 agagcatttt tattagcatc attcccagta gcaatgactg cttggactca accaggtgtt
    12061 aactggatag gtaaaaattt agatgcagtt actacagcaa ctccattaag ctttttgaaa
    12121 aatggagcag caggactggc agacctttct agcaatggaa ttagtcttgc tgacatgatg
    12181 atagggaata ttggtggatg tataggtgag acatcagcaa tattattatt attaggtgga
    12241 gtatacctaa tgtataaagg cataataaat tatgttatac cagtatttta catagcaact
    12301 gtatttatat taacattcct tttaggtgga tttaatataa ctttcgcaat atatcaactg
    12361 tttgctggag gacttatgtt aggtggattc ttcatgctta cggattatac aacttcgcct
    12421 atgactaaaa aaggtcaaat aatatacgct gtattagctg gtcttataac aactgttata
    12481 agaatgtatg gtggatatcc agaaggtgta tcttactcaa tactattagt gaactgtctt
    12541 gctccactta tagataagtt tgttagaaat agagtatttg gggaggtggc taaataatga
    12601 acagtatggt gagattaggt ggtactttac ttgctataag tgcaatagct gcattagctc
    12661 ttggagctac taatcaggtt acagctccag taatagaaca aagaaatata caagcaaata
    12721 atgaacttag aaaagcagtc cttccagaag caaaagaatt taaagaaatg aaggaagata
    12781 cttacaaagg ccttggagat gatcttatag ctgaagtgta tgaaggttta gatggttctg
    12841 aagtggttgg atatacttta aaagctaaac caagtggata tggtggagaa attgaagtta
    12901 tggtaggtat atcatctgaa ggtcaagtta caggtgttga cattggtaat atgtcagaaa
    12961 cagcaggtct tggagcaaaa gctaaggatg atgcatttaa gggtcaatac aaaggaaaaa
    13021 ctgctgagcc tcttgaagta gcaaaaggaa gtacaacagc agataatcaa atattagcaa
    13081 tatcaggtgc aactattaca tctacagcag ttactacagg agtaaatgct gctatagatg
    13141 ttttcaatag tgctcttaat aaataaggag ggttaattat gaatttagct aaagtattta
    13201 aaaatgggct aatagatgag aacccaacat ttgttcaagt tataggtatg tgtccaacac
    13261 tagccgttac aacttcagca ataaatggaa ttggtatggg tctttcaaca gcagcagttt
    13321 taatatgtgc aaatttagtt atatctttaa taagaaaaat tacaccagat aagataagaa
    13381 tacctatatt tatagtagtt atagctacat ttgttacaat agtaggtatg cttttaaaag
    13441 catatgttcc tgctcttgat aaggctcttg gtatatatat accattaata gttgttaact
    13501 gtttaatatt agctcgtgca gaaagttttg ctttcaaaac aggagctatg ccttcaatag
    13561 ttgatggtgt aggtcaaggt cttggattta ctgttgcact tacaataata ggtgcagtaa
    13621 gagaattact tggaaatggt agtttatttg gaatgacatt atttggagca tctttccaac
    13681 cagtattaat atttatatta ccaccaggtg cattcttaac attaggattc ttatttgcag
    13741 gatttaataa attaagaagt aaaaaagcat agtggaggtg taataaatga atttaatact
    13801 tttattttta agtatcgttc ttgttaataa cgttataaca tctcaattct tgggtatatg
    13861 tcctttcttg ggagtttcta agaaggttga tacagcagta gggatgggag ttgcagttac
    13921 gttcgtttta actctagctt caattataac ttattttata cagattttat tagtaaaaac
    13981 tggaactgga ttcttacaaa caatagcttt tatattggtt atagcttcta tagttcagtt
    14041 cgttgaaatg gtaattcaaa agatgagtcc atctttatat caagcattag gagtttattt
    14101 acctcttata actacaaact gtgcagttct tggtatagct ctagttaatg ttcaaaaaag
    14161 ttataattta gtagaaacaa taataaacgg atttggagcc ggagctggat ttactttagc
    14221 aatagtaata ttcgcaggta taagagaaag actagaatta gctgatatac cagaagcatt
    14281 taaaggtttc cctataacgc ttatatcagc aggtttaatg tcaatagctt tcttaggatt
    14341 tacaggactt ataaagctat aaaaaagagg ggtgaaaatg gtgatactta cagctgtatt
    14401 agttttagga attatgggct taatcttcgg gatagtacta gattttgcat ctaaaaagtt
    14461 tgctgtagaa gtagacgaga gagtggaagc tatactagga gtacttccag gagccaactg
    14521 tggaggttgt ggatttccag gttgtggagg tcttgcaaat gcgatagttg aaggcaatgc
    14581 accagttaat ggttgccctg taggtggagc agatgttggt gcaaaagttg gagaaataat
    14641 gggaataagt gctgaagcag gtgagaaaca agtagcaaaa gttatttgta aaggaacttg
    14701 ttcaagtgca aaagataaat atgagtatga aggaatatca gattgtagag cagcaaatgt
    14761 tttaaactca ggtgctaaga tgtgtaaatt tggatgttta ggtcttggaa catgtaaaga
    14821 tgcttgtaaa tttgatgcta tttctatagt tgatggaata gcagtaatag atgaagaaaa
    14881 gtgtgtaaat tgtggaaaat gtaaagaagt ttgtcctaag ggtataataa tcacaaaacc
    14941 agaaagtcaa gaagtagttg tagagtgtaa tagcaaagag tttggtaaag ctgttaagga
    15001 aaaatgtact gctggatgta ttggctgtgg aatgtgtgta aaagcttgta aatttgatgc
    15061 aataatattt gaagacaaaa tagctaagat agaccctaat aagtgtgtag ggtgtatgca
    15121 atgtgtagca aaatgcccta caaaagtaat atcaggtgac ataactaaga aaaagaaagt
    15181 tactattgac caagaacttt gtgttggatg tacagtgtgc aaaaaacaat gtaagtttga
    15241 tgcaatagag ggagaattaa aagaaaaaca taaggtagat gcagataaat gtgtaggatg
    15301 ccatttatgt atggaaaaat gtccaaagaa agctattaaa attttgtaat atggacaaaa
    15361 taaggatatg gaataatttc catatctcta atgagtacat aaaacagagg tatggaataa
    15421 tttccatacc tctattttat gtatatttat tctaatggta ataaatataa caatagtgta
    15481 acaaaaaagg ttcgacattt ctgttgaatt ttttacaata tagtgataga ctaaataggt
    15541 acaaattacg tacaaatgag gtgaataaat gaatataatc ttggcatcag cctctccaag
    15601 aaggaaagaa attctagaga atacgaatgt aagatttgat ataattaaaa atgaaattga
    15661 tgagattata ttggaaggtg aaacaccaaa acatttagtt atgagattag cttttgaaaa
    15721 aagtatgtct gtagcatctg aacataatga agatgttgtt attggagctg atacagttgt
    15781 tgttttagac aatgctattt taggaaaacc aaaagatgag tcttgtgcaa gagatatgtt
    15841 aaaaagacta tctggaagag agcatcaagt cataacaggt attagcttaa taaacttgtg
    15901 cgaagataaa aaggtaattg actatgtaat cagcaatgtg aaatttaaaa cgttatcaga
    15961 gcaggatatt gaggattatc tgaaaactaa tgagtcattt gacaaagcag gagcctatgg
    16021 tatacaaggt tatggagctt tgttagttga agaaattaga ggagattatt ttaatatagt
    16081 tggtcttcca atttcaaggt taggcgacct attaaaaaaa tattttagta taaatctttt
    16141 ttatggagtg tgatttatct gaaaaagtca tttaattcaa ccattaaggt aaaagaaatg
    16201 gcaccagagg aaagacccag agaaaagatg cttgccaaag gcgtaaaaag cttatcaaat
    16261 gctgagttgt tggctatact tttaagaact ggcaacaaga ataaaaatgc tattgaatta
    16321 gccaactata taataaatag agatattcag ggtattagac atttagaaga tatgacaata
    16381 gaggagctgt gtaatataga tggaattggg ttatcaaaat caacccaaat taaagcagct
    16441 ttagaactag gctctagagt agctagtttt aaacctataa agtataaaat aaggaatcct
    16501 tgggatatac agaggtatta tatggatagc ctaaggtatt taaaaaaaga ggtttttaaa
    16561 gcggttcttt taaacacaaa aaatgagata atatctgatg tagatgtatc tatagggact
    16621 ttaagctcgt cattagtcca tccaagagag gtttttaaag aagctataag aagaagtgca
    16681 agtaagataa tagtgatgca taatcaccct tcaggaagtg tagaaccatc aagagaagat
    16741 aaaaatatca catcaagact tattaaatgt ggagaaataa ttggaataga aataatagac
    16801 catataatta ttggagatgg attatacttt agctttaaag aaaatatgat aatttgactt
    16861 tatatgtgat aaaataatag gagaatgttt taaacatgat tcttaatagt aggaaggagt
    16921 aaaatcatgg ctaaggaaaa aaagaaagaa aaaaaaggtt tttttagttt taataagatg
    16981 acaaaggata tgggaataga tttagggact gcaaatacat tagtttacat aaaaggacaa
    17041 ggtattgttg ttagagaacc atcagttgtc gcaataagag atgatagtaa agaggtatta
    17101 gcagtaggtg aagaagccaa aaaaatgata ggtagaacac caggaaatat agtagctata
    17161 agacctatga aagatggagt aatagctgat tttgacataa ctcaatcaat gattagctat
    17221 tttatacaaa aagcggctga taaaaaaggt gttgtaagtc caagaatagc tatatgtgtt
    17281 ccttttggag ttacagaagt agaaaagaga gctatagaag aggctgcaag acaagcagga
    17341 gctaaagatg cattccttat agaagagcca atggcagcgg ctataggggc tggattaaaa
    17401 gttgaagaac cagaaggtaa tatggtagtt gatattggtg gaggtacttc agaaatagct
    17461 gtaatatctt taggaggaat cgttactgct aaatctataa gaataggtgg agacgaattt
    17521 gacgagtcta tagtggctta tgtaaaaaaa gaatataatc ttatgatagg tgaaagaact
    17581 gctgaaaatg taaaaattaa cataggttct acatttaaag atgatgaaga aataaatatg
    17641 caaataagag gtagagattt aatctcagga ttacctaaaa caatagaaat atgctctaca
    17701 gaagttagag aagcattaaa agaacctgta agttcaatag tagatgcaat aaaatctaca
    17761 ctagaaagaa ctccaccaga gttagcctca gatataatgg aaaacggaat aatgctaaca
    17821 ggaggaggcg ctttgcttag aggtcttgat aagcttatta cacaggaaac tggtatgtct
    17881 gtacaaatag cagaaacacc tcttgattgt gttgcacttg gaacaggtaa atctgttgaa
    17941 gaccaagaaa tctttgaaaa agtattaatg atgaatacta agaattagaa gttggtgatg
    18001 gccttgagat ttgacaagaa caaaaatgag aaaaaaagga ttaatgttaa agtgatagca
    18061 acgggcgtag ttgctatcac tttaattgga attgtgggta tatcaattgg taaattttct
    18121 agtggtagtc cagtaaattt aggagtagct tcagatgcta ttacaagtgt aggtaaggga
    18181 ataaatgatg gattttcttt tatcaaaaat ggttttaaag atgtagcaaa ttttaaagat
    18241 aattctaaaa aggtaaaaaa attagaagaa gaaaatgaaa aacttaaaaa aaatatgata
    18301 gctttaaatg ccaaactaga taaaacagaa tcgctagaag agttaaaaaa gacactgaac
    18361 tttgtacaag aagagtataa ggcgacaagt atttcagcaa gtgttgttgg taaaaatgat
    18421 ggaaactggt atgagagttt tgtcataggt gcaggaaaaa attcaggtgt aaaaaaagaa
    18481 agtatagtta tgaatggtag tggattagtt ggcatagttt atgaagtttc caacaattat
    18541 agtaaggcaa tttcactatt ggattctaag gcatctgtaa gctttaaact agcaaaggat
    18601 gccaatgcta aaggtacaat tactcaaaat actactttag ataataaaga tagttataat
    18661 agtaaaggat atttgcaagg atatatgttt gactcatcat ataatgtaat tcaaggagat
    18721 ataataacaa catcaggtct tggattcttc ccagatggta taccaattgg cgaagttgaa
    18781 aaagtggttg atgataaaga taagtcatta aaatatgtag ttgtaaaacc ttatgttgat
    18841 tttaagaata taaatgacgt tgtagtcata gaaccaagaa atataggata aaggggataa
    18901 aaacaatgaa aaaagtttta ctttgtctgt tggggattgt gcttatgact atagaaaata
    18961 gtataacaaa ttatatagat atttttggca taagctttaa tttagttctt atatatgtaa
    19021 ccataatctc tctttattta gatgaattag aaggtgggat tatagcagca ataatagggc
    19081 ttattaaaga cataaccgtt ggtggaattt ttggtgtaaa tgcattaatt ttgtttatta
    19141 ttgcatatgc aattggatac atgagagatc agctatataa aggtagttat attattactt
    19201 tcgttttaat ttttataggc agtttaattg attcaattgt caatataggt acatctagta
    19261 taatttatca aagctacaac atatcaacac tttttgtaaa aggtctacta gtagcaccaa
    19321 tagtgaatag tttaattggt ttagtaatat atcgcttatc taaaagagca gttttaaaac
    19381 ttaaagaaga ttagttggtg atttcatgga agaaaataaa caagttgata gactaaaaat
    19441 aattaagata gtcatgggcg tgatattttt agctatttta gtaaaaataa tctatatgac
    19501 tacatttaaa tatgaatatt acaatgagtt agctgagaat aaaacatata aaaagttggc
    19561 aatagaagct ccaagaggag aaataaaaga tagatatgga agactgttgg caggaaataa
    19621 aaacttattt actgttcaag tttcaggtaa cgatattaat aagaaagatg caaataagca
    19681 ttcaagagca aatgaaatat cattaaaact tataaatttg ctggagagaa atggtgaaga
    19741 atatgtagat gagtttccta tatatgtaga aaatggaaag tactattata catatgacag
    19801 agatattagg gaatataaat cagaaaatgg tattccaaat gattataatg ctaaggaaag
    19861 tttttattac ttggtagaca aacttatatc agctggaata ttatctcaag aagataaaag
    19921 attggatgcg acaagattac aggcaaaact aaatgaaaat ggatattatc cacctatttt
    19981 agttagtaaa tggatgttca cagcagaaag agataagaga gattggttgg ctagttataa
    20041 gataaaagaa actaaactga gtgccaaaga agcgtttgaa aaagtaagaa atagtgatgc
    20101 attagaaatt gataaaagtt tgagtgatga agatgcaaga aaaataatgg tcgtaaggga
    20161 tttgataaaa tcaaaaggat attctcaata taatccagta actattgcta aggatgtggg
    20221 agaaactacg attgctcaaa ttgaagaaag tgctatggac cttgttggtg tttcaatagc
    20281 agtagaacca gtaagatatt atcctaatgg gtcattagct tctcatatgt taggatatgt
    20341 aggaaaaatg ccatccactc aaatagaatc ataccttcaa aaaggttatg aaactggaga
    20401 tatggtaggt cttgcaggag tagaaaagag taatgaaagt agattaagag gaactgatgg
    20461 ttataaaatg gttaaagttg atgcattggg tagaatcagt aaagaaattg aatctaaaaa
    20521 acctaaatca ggagatactg tgtatcttac actagataaa gatttacaag aagtttcaga
    20581 taatgcatta aaacagataa tcgaagttgc ttctaaagga ggtactttta aaagtaaatt
    20641 tggggacaaa ccaattagtg catatgcagg aaaagcacag tcagcagctc ttatagctat
    20701 tgatgttaaa aatggagaag tattagcttc atcaagttat cctaattatg acccaaataa
    20761 atttgcaaaa ggtatttcta cagaggatta taaagcactt caaccaaaaa atccaaatga
    20821 cttactagca ggaagtccac ttttaaactt ggttacacaa ggggaatttc agccaggttc
    20881 ttcatttaag atggtaactt caatggcagc gttagaaaat ggtctggatc caaactttac
    20941 tataaatgac cctggggtta taatgttagg taaaaaatct tttggtgact atgtatggaa
    21001 ccatggtaga ggaaaccatg gtatgacaaa cttatataaa gcaatacaag agtcttgtaa
    21061 tatatatatg gcaacaattg gtacaggtaa aacatggcca gatggaaaaa gcattggtat
    21121 agatatgaat gcaaataaaa tacttgagta tgcaaaatta tttgggcttg accaaaatac
    21181 agggcttcaa gatgaagtag aagaaagagc aggtaaagtt cctagcacag aagataaatt
    21241 gaaatcaaca caagctcttc taaagtcaaa tttagaaaga gaaatggcaa atgattttgt
    21301 tgatataact agagaaaaaa atcctaaaga atatgagaaa agaatcaatg agattgtaag
    21361 ctgggcagca gaaaagaaaa ctcctggtag agttgaaaca atgaataggc taaaaaaatt
    21421 ggatgttaaa gaagatagaa ttgaagaagt agcagattta gcagtattca gttattttaa
    21481 ctttgcaaaa tggagtactg cagatacatt caacttagct ataggacaag gtgagaatgc
    21541 ctatacacca gctcagatat caagatatgt ggctgctata gcaaatggag gtaatttagt
    21601 agaattatct gtagttgata gagctgtttc aagtgattat tcatcagtta aaattaatga
    21661 tcagaagaaa gtcgaaaaaa taccttttaa aaatccagat aacttaaaag aattaacaaa
    21721 aggtatgaaa ttggttgcaa gacaaggtac agcgaagagt gcttttgctg attttccaat
    21781 agatgtagca gcaaaaactg gtacagctga gaaaagtggg aaaataccta cagataacga
    21841 gtatgaatat ttaaaaagtc atatgtcatc ttataatgtc aacctaaatg atgcgattaa
    21901 actagctgat aagatgaaag cagaaaaaga aaaagagctt tcattagcta aggaaaagga
    21961 aataaaaaag aaattagaaa ataaagattt gaaagacgaa gaaagaaaga aattagagga
    22021 agaattagaa gatggagtaa aagttagatt agaggacact gataaggtga attcttcata
    22081 tctaagaaaa gctataaaag aattaaatcc aaaaataaca gatgaccaaa tagatagatt
    22141 taaacaagat tatggttctt ttacgtggac tgttgctttt gctccagcag atgacccaga
    22201 aatagctgtt gtatgtgtaa ttcctcaagg tgattcaagt gtattctcac ttttaccaac
    22261 tagagaagtt attggaactt atatgggatt agagccaact aatagtaaaa atgacaacaa
    22321 gacgaatgat gttaacaatt cttctgacga aaatataaat ttcgagtcac aaattaatag
    22381 atagctcaat ctatgtataa actttagctt tatatagaaa aaataaaagg gtttaaagcg
    22441 tatttgaaga atataaatga tatatcactc tggaggttgt tatgtcttta agagaaattt
    22501 gtagtcaaga actagtagag ttcaagggta ataaaagagg gataatagta aatataaaaa
    22561 gagaagctcc atttgaggaa atacaggaaa aaataataaa caaattggaa gcatatgttg
    22621 gcttttttaa tggggctaaa attagtaaaa ttaatagtga ttgtttaact gacatggaga
    22681 ttctagaact taaagaagga attacttcta gatttgatgt ggaatttgtt gaagatcaaa
    22741 aaattgaaga aaatagtaat tttccaacaa aatatgtcaa taccctaaga tctggtgaaa
    22801 atattgaatt tgaaggtgat gtagttatat tgaatgatat gaaacctggt tcaaaagtcc
    22861 tttcaaaatc aaatacagtt gttatgggag atataaatgc tggtgctaag gttgtagcag
    22921 gaggtaatgt ttttgtaatg ggtaaaatag aaggttttgt acatgcaggt gcagaaggca
    22981 atgaatttgc atatgttgtt gctggaaatc ttaatcctaa aatattacaa atagcagata
    23041 atattgcaga agctccagat gatgaagaaa attatgaaag tgaatcagaa attagcccag
    23101 aaatagcctt tgtaagtaat ggaagaattg taatcgaaag ctatttgtca aagttagaca
    23161 aatagaaaga aaaatagggg gtagtattat gagcgaagtt atagttataa catctggtaa
    23221 aggtggggtt ggaaaaacta ctaccgcagc aaacttagga actgcgctaa gcctagaaaa
    23281 taaaaaaaca gtagttgtgg atgcagatat tggacttaga aacttagatg tagtaatggg
    23341 tcttgaaaat cgaatagttt atgacatagt agatgtagtt gaaggaactt gtaggcttaa
    23401 gcaagctcta ataaaagaca aaagatttga taatctatat ttattaccag cagcacaaac
    23461 tagagataaa aatgctgtct cagttgagca gatgattgac ctgtgtgaga aactaaaaga
    23521 atctttcgag tatataataa tagactgtcc agcaggtatt gagcaaggct ttaaaaatgc
    23581 agtagctgga gcagatagag ctattgtagt tactaatcca gaaatatcag cagtaagaga
    23641 tgctgataga ataataggtc tattagaagc aaatgaaata aaagagataa gattagttat
    23701 aaatagaatt agaaatgata tggttaagcg tggagacatg atggataaac aagatataat
    23761 agaaatatta gcaatagatt tgttaggtct tgttcctgat gatgaaagca taattatatc
    23821 aacaaataaa ggagagccag ctatacttga ttctaagtca cttgctggtc aagcatacaa
    23881 aaatatcgca aaaaggatac taaatgaaga agttcctcta cttgatcttg aagttgaaga
    23941 tggattcttt ggtagactta aaaaaatgtt tagcatggct aagtagcaaa ggaggataca
    24001 tctgtgttag atttatttag agttttttct aacgaggcta aaactagtaa atctgttgct
    24061 aaagagaggt taaagctagt tttagttcat gatagagtag attgttcacc acaactttta
    24121 gagatgataa aaacagacat tttaaaagta atagctaatt atgcagaaat agaagatgat
    24181 ggccttgaaa taaaaatgtc taaatgcaga ggtgagcatg atgacaaacc tgtatctgca
    24241 ttagttgcta atataccact aaagaatata aaagatagat gtatgtaatt agtgcttaaa
    24301 agggggatat ccttattggt atatgcccct tttatttaat tatatgttta aagatagaaa
    24361 gacggtgatt ctttgaaaaa aatcaatatt aattataagt cgacaattaa gttgataaaa
    24421 cagttagact ggaaacttat agttacagta ttagctatct ttatttttgg gcttgttata
    24481 cttagtagtg caacacatgc taattcaaca ggttcatata atcaattgat taaacaagga
    24541 ttagcttttg tacttggtat aggaatgatt atagtcatat tattttttga ctataacctt
    24601 ctaggcaggt attataaagc gctttatata ataagtctta ttttgttagc catagtttta
    24661 ttacctggaa ttgggacagt aaaaggtgga gctaggtcgt ggattaactt aggaccactt
    24721 gacttgcaga catctgaaat agtcaaatta acatttgtac taagttatgc aaagatactt
    24781 gaatccaaga aggataaatt aaatacgtta aaggaagtta tgcctgttgt agtttattct
    24841 cttccattta tcggattatt gattgcacaa ccagacttgg gaacaggaat tgtattttgt
    24901 tgtatgatat ttgcaatgct gttcacagca ggtcttagct caaagttgat aaaaagagga
    24961 ataatcatac tattagtgtc aatgccactg atgtatttaa tgatggcaga ccatcaaaag
    25021 gtaagaatag aagcttttct aaatccagaa gatgtaactc taaaaggaaa ctatcaagtt
    25081 atgcaatctc tgattgcaat tggttctgga ggcgtaactg gtaaaggact ttacaatgga
    25141 agtcaaaatc aagaagattt tttacctgta caagatagtg attttatatt tgctgttgta
    25201 ggtgaagaac ttggtgtaat aggaatggca gtgttgataa tcttatttat gatattccta
    25261 ttaaggcttt tagcaattgc aagagatgca aaagattttt atggaaccct catagtggtt
    25321 ggtgttatgg gaatgtttgg ttaccagata atccaaaata taggtatgac ggttgcgtta
    25381 ataccagtaa ctggagtaac attacctttt gtaagttatg gaggaagttc gttattgaca
    25441 tctttagcaa acttaggatt agtactaaat gtgtgtatga gaagaaagaa aataaatttc
    25501 taaatttaaa tacatctaaa cataaattga ggagattaga tatatgaata tagcattagt
    25561 agcacatgac caaatgaaaa acactatggt cggattttgt ataggttatg aatcaatttt
    25621 aaaaaagtat ggactatatg caactggaac tacaggaaaa agaataatgg atgaaactga
    25681 attaaatata aatagattag catctggtcc tttaggtgga gaccaacaaa taggttcttt
    25741 aatagtaaca caagaaatag atttagttat ttttttaaga gacccattga catctcaggc
    25801 tcatgaaaca gatatacaag cgttaataag actttgtgat gtttatcatg ttccaatagc
    25861 tacaaatcta gcttcagcag aaatatttat aaaagctctt gatagaggag aactatcatg
    25921 gagagaagtt agaaaaagca aaagtcaacg tatttaaaaa atattaatat tacagtctaa
    25981 agaaacagat atataagtgt ttaatgccct agtttttctg ttggaataaa tctagtacct
    26041 gtagaaatct tatgaaagat ttaaatgatg aagagctaaa tttgagatta aataattagg
    26101 aaatagatta tagatagagt agtagaattt aaactattac tctattttaa taatatgaaa
    26161 tagaatcaag tctattatta tatatattta aacttatagg taatactatt tcaaccaagt
    26221 aaatgttgtt atgacaacat taataaaaca aaaatttatt tttaaaattt gataaatagc
    26281 tgtatattag tatttataga ttaaatatta atatacagct atttattatt tttaagtcta
    26341 ttgtagaata taaaccttgt attatagagt actatgtatt attatagtaa atataagtac
    26401 tatacattat agagtagtag gtgaaaaggc ggtggtacat aatgcagtta aataaagaag
    26461 tgttaaaagg ttatatcgat atattaattg tttctatatt agaaaagaaa gattgttatg
    26521 gatatgaaat tgcaaagcaa gtaagagaaa gaagtgagtt tgaattaaaa gaaggaacaa
    26581 tgtatcttgc acttaaaaga atggaatcta aaaatttaat taaatcttat tatagcaatg
    26641 aacaaagttc aggtggaaga agaaaatatt ataatttaac taatgaaggt aaagattttt
    26701 tagaaataaa gaaacaagaa tggagattta ttaaaaaggt tatgaatcaa tttttgggag
    26761 aggtggatta atttatgaaa ctaatagatg aatatttaga taaactatat aaaaaatgtg
    26821 acaataaaag tactatagaa ttaaaacagg aaatgagatg ccatcttata gaatctgcaa
    26881 atgaatttaa attagaagga ttagatgaag aagaagcatg taaaaaagca attgaaagat
    26941 ttgatgatgg tgatgaaatg cagtatgaat tatgtaatat aataaaagaa ctatctttat
    27001 cattggatag gcataaatca attgtaatgg ggtttaaaaa agtattaggt tacataagta
    27061 taattgcatt tttaatttct ggatttatgt ggtattacaa taatagttta caacataata
    27121 tgtataattt aggaaaagaa cttgatggag aaataaagca attggcagaa agacatgata
    27181 tgacaaagat tggtgaatat aagttagaac ttgaaaaaat acttgataaa gataagtata
    27241 gtaaagtcaa agctcttaga ctatatgtaa tagatatgaa agatggaaac actaatttaa
    27301 gttcttctgg attgaatgct aatatggtat atgagaggga agcggattat aataacataa
    27361 gtaattttat acagcattta ggatataatg gaaaagattt tttagacaag aatgggaata
    27421 tagtaaatcc agatattttc ttagaatatt tcttttattt tgaatcggag atgttaattc
    27481 cagtagcatt tgcatttgga ttattatgta taatagcgta ttttatatta agatttaaaa
    27541 tttctttaat aaaaaataat aattaaataa ttcaccaaat aatcttaatt tgtagagtaa
    27601 ataagatgtt tcaaaataaa atttgaatca tcttattttt tattgtttgt tatactcttt
    27661 tcttttatat ttctccatag tggattgtat ctatacataa tcacatattt tgatataatt
    27721 atactttcca aaaaatgtat tgaatataca aattataatc tgtataatgt tgttagggga
    27781 gaatgtatga agtgtataca attttaattt taaatgtatt attgacaatt ctcctcactc
    27841 tcgccattac caataattgg aaaattgaca tacctgttac agaccaacat ttttgaatat
    27901 attaattatt atcattttat tgtgatgata tcaaaaatgc aggtttgtga gttggattgt
    27961 atacgaacta tataggaaat atagaacaga ttttccaaga aatctaaata ttctatgacc
    28021 acgtgaattg ctatgcatac atgttgataa tccatatttt aaatctaaaa tgaacttaat
    28081 taatggtagg gaattgaaag taaaatatat tataaaggag aatgctaata tgaaaaaaat
    28141 caggaaatta gctactaaac tattgattgc aacgatggtt ttaacctcat gtatgagtgt
    28201 aacagtttat ggtatgacag ctaaagatgt tactgcaaag acaccgaaaa gttatgtagc
    28261 tggaaccaac agtatatatg gtccaaagtt atcacaagct caacttaatt cggtagcaca
    28321 agcaacagca gactttatga acaagaaaat tacaaagaac atgaccacag atgctaagat
    28381 tagggttgct tataatcaca ttaaaaacaa cacaacatat gttaactgga acgcagcaga
    28441 gggagccaat actgcttata cattggttac taaaaaaggt gcttgttctg gaatgtgtag
    28501 gagcttgaaa gctttatgtg atgctatggg aattgaatgt tattatgtac atagtacaac
    28561 aaatgaccat cagtggaatc ttattcgttt tgaggatgga cgtttgtatc atgtagatat
    28621 tgatgccaac aaatcagcag gagcagacat catatacaag agtcttactc acccattacc
    28681 atttgacaag aaagcatatc cagctgttgg cgaagtagta aagacacaga atccaacgcc
    28741 aactgttaag aaacctacgc cagctaagcc ttctgcgcca gttggacctg ggaatcacac
    28801 tatgatagca gatcaacaag gggcaatact tgatgaagtt tatggaaata atctacgtcc
    28861 aaaaattgaa atgtatgagg gagttccgtg tagtttaatc aactcatcaa taaatacaat
    28921 taagtttaca agcaaaaatt caaatttaga cccatcaaag catacagtta ctgtaatagg
    28981 gtttatgaat acaccatatg gtatggttgc attagctcca gatggtgtac ctgatgagta
    29041 tccttttgaa tatgataagc cattttcttt agattttact ggtaagaacc tttctgcagc
    29101 agaattatca aaaaagtgta aagaaaataa tgtaattatt aaagtggaaa ttggtgaaac
    29161 ggcatctaat tctggaagag ggatggtagc atacgctaga tatgtgaatt aatacaaata
    29221 tactagtgta caaatagaac atattataaa taatgcaccc tcgcacaaag ggtgcattta
    29281 tcattatatc agtcatttta gaaaataaaa aaggagttga acttaaaatt agattagaaa
    29341 aattaatttt gagacaactc cattttaatt tatttttaaa tataaaatca tttattttaa
    29401 ataatattat gtgtgattaa tggctattta actaattttg caaattctgc accaaatgcc
    29461 atacactctt ttaatgtctc ttcactaggg aagaattttt tcttaagaga ttctacaggc
    29521 attttgaatg acatagattt aagtaaagtc tcagccatag ttataccttc accactccaa
    29581 ccaaatgaac caaatactcc tgctatcttg ccttggttag ccattgggtc tataacagag
    29641 aataaatccc acataggttt aaccatagtt ttgtttatag ttggagagcc tagtaatatt
    29701 acttttgaca ctactaatgt atcgtgcatt tctgttaatg tcatatcttc taaatcatat
    29761 aattcagcct ttgcaccttc tttatcaagt ccttctttta tctttttagc catctctaaa
    29821 gtattactat aagctgataa gtagaatata gaaacttgat tttgattagt tgtattaaca
    29881 gcttctgtag accactcaac atatctttta acagcagcca ttgggtcttt agtaagcatt
    29941 ggaccatgag aagtcaatat agtatcaaat tctatgttta gtcctaccac cttatctaca
    30001 gcacttaaaa catgtttagc aaatggctta actatacaat catagtagtg tttagctgat
    30061 tttagataat cttcactatt cactacctct gcatcaacac ttgcaaagtg acaaccaaat
    30121 gcatcacaag ttaaaagagt cttgtcctcc tcaatgtaag taaacattgt atcaacccaa
    30181 tgtaagaatg gagcagttat aaatctaaga tttcttttac cgatatttaa aatttcacca
    30241 tcttttatta catgacagtt aaaaggtcta tttatttgac catctaagta tagtttagca
    30301 gctttagtac aataaacttc tatattagga tttatatcta ataagtattt taaactacct
    30361 gcatggtctg gttctgtgtg atgaattaca acatagtcaa ttttagctat atcagtaacc
    30421 tctgataaat tacttaaaaa ctcatcttta aaatttgcct ttacagtgtc aaataaaacc
    30481 gttttttcgt ccttgattaa ataagagttg taagttgtcc caaactcagt ttccattatt
    30541 atgtcgaata cttttaaacc ttcatctact acgccagtaa agtagatatc tttttttact
    30601 tcaaaaactt tactcatttt taaccctcct aaaatttatc attatataag taacataccc
    30661 cattttgagg tgaatgaaac aaaagatgtc atgataagtc atgtaaaaca atagttttat
    30721 gtttataata tatcatactt tgtgaaaaaa ttgtatttca ataaaaattt aatagaaaat
    30781 attcttaaat atttttagct attctatttt gtgaataata tgtattttaa attcaatatt
    30841 ttagttatat ttttgataga aaatatttta tcgtgtataa tattacttat aaataagaat
    30901 aaaaataaag agcttgtttg gaggaatttt aataaatgaa taaagtagac ttgaaaaaaa
    30961 tcttaaaaaa agtagaaaag cctgctagat atcttggaaa tgaaataaat tcaattcata
    31021 aagatactag taatagtgaa ttgataagat atgcacattg ctttccagat ttatatgaag
    31081 ttggtatgag tcacttaggg agtcatatat tatatgatgt aataaataag gatgaagatg
    31141 tattttgtga gagagtgtat tctcctgcag ttgatatgga aaatataatg agagaaaaaa
    31201 gtataccatt atttgcatta gagtcaagag agcctataac aaactttgat tttgtcactt
    31261 ttacacttca atatgaactt tcatacacta atatcttaaa tatattagat ttagccaata
    31321 taccaatatt aaaagaagaa agaactttgg aagacccatt tataatggtt ggtggtcctt
    31381 gtgcttataa ttccgaacca ttagcagatt ttgtagatgt agtaatatta ggtgaaggtg
    31441 aagaggttaa tttagaagta gtaaatgagt ataaagaatg gaagaaaaat aaaacgacta
    31501 gagaagattt tttatataaa atatctagca tagaaggagt atacatacca agtttttatg
    31561 atgtcaagta caatgaagat ggtacagttc aaagtgtaac tcctaataga gaagggatta
    31621 ctaaaaatcc tactaaaagg ataataaaag atgttgagac tgtagattat ccagaaaaac
    31681 taatagtacc ttatatagat actgttcatg atagaatagt actagaatta tttagaggat
    31741 gtactagagg atgtagattc tgtcaagcag gtatgatata caggccaata agagaaaaga
    31801 gtgttaaaag acttaaagaa atattagata aattagttaa gaatactgga tatgatgaaa
    31861 tatcattatc atcacttagt acgagtgatt atagtaagtt atcagagttg actgattatt
    31921 tggtagatga atatgcatct aataatattg ggatttcgct tccatccctt agattagata
    31981 atttttcaat ggaaattgct gataaaatac aacaagtaag aaaatctgga cttacttttg
    32041 ctccagaagc aggaactcaa agacttagag atgttataaa taaaggtgtt acagaagaag
    32101 atttggaaaa tgcaacagaa agagcttttg agatggggtg gaatagtgtt aagttgtatt
    32161 ttatgattgg tttaccaact gagacttatg aagatttaga tggtatagct aaattagctt
    32221 ataaagttat tgatatttat agaaaagtaa atggaggaaa gctaaagaga agttttagtg
    32281 ttacagtgag tacttctaca tttgtaccaa agccatttac acctttccaa tggcatggtc
    32341 aagatactac agaagaagtt attaataagc agagacattt agtaaataaa ttaagaaata
    32401 acaatataaa atacaattat catgattcaa agactagttt gatggaagca gtagtagcta
    32461 gaggcgatag aagaatagga aaagttatat atgatgcatt tagactaggt gctaaatttg
    32521 atggatgggc agaacatttt aatttagata tatggaaaga agctatggaa aagaataatc
    32581 tttctataga tttttatgca catagaaata gaaactatga agaggtattt ccttgggacc
    32641 atatagatgt aggtattagt aaaaaattct taattagaga agacgaaaat gctaaaaaag
    32701 aaaaaatcac ttctgattgt agacataact gcaatggttg tggaataaat atacatgata
    32761 taggaagggg gctttgctaa tatgagtaag ataataagag taaaatttaa aaaggaaggc
    32821 gacatgatat atatatcaca tcttgacctt cagagattgt tacaaagagc gtttagaaga
    32881 gctgaaataa acttatcaca ttctcaagga tttaatcctc accctaaaat gagttatggc
    32941 aatgctttgg ctttagggac agaaagtcaa ggtgagtatg tagatattga aatagaagaa
    33001 gatgatttat cagtagagga atttttaaat aaagtatcaa ttcaacttcc agatgggata
    33061 gattttatca aagcaaaaga aattgataga caaacacctt ctctttcttc tgtgatagac
    33121 tatggagaat atttgtttaa tatagatttg aaaagaccta tgacgaaaga gtttgtaaaa
    33181 agaaaagtaa ttgactttat gaataataaa gaaataatca taacaaaaaa gaataaaaaa
    33241 ggtaagatgg ttgaagtaga tataagacct atgataagaa cctttgatgt tttaaattta
    33301 gaagatgaac atattactct tactgctact atagctacag gctcaaaaac taatttaaat
    33361 actaatatac ttataccaaa gatattagag atgtttgaac ttgatattga cccattagat
    33421 gttgatatat tgagaagaga tttatatgta ttagaagatg gggaattagt gactcctatg
    33481 taagctcgag aggtgaagtt gtgaaaaaga tagttattga gtccctaata gggtcccaga
    33541 aaacagcagt acttgaagat gaaagattaa cagaactttt tgttgaagac aatttaaata
    33601 agaagactgt atccaatata tatcgtggaa tcgtaaaaaa agttatacca ggaatagaag
    33661 cttgttttgt agatattgga tttaagaaac ttgcttattt acaattaaaa aagggaagta
    33721 ctataaaatc tggtcaggat attttggttc agataaataa agaagaaata ggaactaaag
    33781 gcgcaaaatt aaacactgag attagtattt ctggaaggta tattgtctat ataccttcca
    33841 atgatagaac tacaatatca aataaaatta ctgatgaaaa agaacgattt agattaaaaa
    33901 aaattactaa ggcagtaaat aaggaaaatc taggattaat aataaggact gaggctcaag
    33961 gttgcactca tgatgaaata aagaaagata tagaagagtt gaaattaaag tatgaaaaca
    34021 ttttaaaaga atataaactt ggtataggtc cgaaattatt atataagtct ttagattttg
    34081 caactaaata tgtaaaagat aatgtgaatg atgatattga atctataatt accaatagtt
    34141 atgataagta ctctgaactt aagtctattt taagaggtat agataagact tatgttgata
    34201 aactgtgtct tgaggaaaat agagatgtat ttgatttata cagaatagaa tctaaaatag
    34261 aaaaattatt aaataaaaaa gtatggttaa agagtggagg ttatttaata attgaaaaaa
    34321 cagaagctct tacggttata gatgtaaata cgggtaagtt tattggaact ggtaaacttg
    34381 atgaaaccgt ttataaaact aatcttgaag ctgcaaaaga aattgtaaga cagcttagaa
    34441 ttagagacat agctgggata ataataatcg attttattga tatgcataaa aagaagcatc
    34501 aaaaggagat tttacacatc ctagaagaag aatttaataa agataaaaga aaagctgaag
    34561 tattaggtat gacaaaactt ggtttagtag aagttgctag gcgtagagaa aaagaatcca
    34621 ttgataagta ttatttaatg tcttgtccat gttgtgatgg tgaacaaaca ataaaatctg
    34681 tacattatat actagatagt attgaaaaag aaattatgag aataagtgaa catactgttt
    34741 acaaaaacat aatggtagaa tttaatgatt ttatttttga acaaataaaa gaatattata
    34801 tggatataat tgataaaatt ggtgaaaaat ataatataaa gatttcttta aacgcaaata
    34861 gtacattaaa gcacaataaa accaatgtta ttttcgataa aatagttgac aataaaatgt
    34921 aaatatttta taattaataa ttgtaatgcc gcacagtaaa ggtttaaatc ttcaaatgaa
    34981 gtaccttgta ctggcgagtt tgaggccgag gaggtgtatt taatgtacgc tatagtaaag
    35041 actggtggaa agcaatataa agtttctgaa ggtgatgtat tattcgttga aaaattagaa
    35101 gctaacgcag gtgatgttgt tactttaaac gaagtattag cttgctctaa agatggagaa
    35161 ttaaaattag gttctccagt agtagaagga gcatctgttc aagctaaagt tgttgaacaa
    35221 ggtaaagcta agaaagttat agttttcaag tataaagcaa aaaaagatta cagaagaaaa
    35281 caaggacatc gtcaatcata cactaagata gttgtagaaa aaataaacgc ttagttattg
    35341 gttaagttat gataaaagtt aaatattatt ataatgatga tttcttgttg caaggttttt
    35401 gtttaaaggg acatgctgat tttgctgaaa taggctatga tatagtatgt gctgcagtta
    35461 catctaatgc tattgcagtt ataaattctt tagacaaatt acaaaagatt gaatttgaaa
    35521 aagtagtagg agaagaaggt catatagagt gtatagtcaa agatacacat gtaaaagacg
    35581 cacaattatt actaaatcac tttcaattag cggttaaaga aattaaacgg gaatacccaa
    35641 aaaatataaa aattttaaaa aagtaggggg tgactccata tgttaaacat gaatttacaa
    35701 ttattagcat ctaaaaaagg ggtaggttct tctaaaaatg gtagagattc tatatctaaa
    35761 agattaggtg ttaagagatt cgatggtcaa ttagtaactg ctggaagtat aatagttaga
    35821 caaagaggaa ctaaaataca tccaggaact aatgttggaa aaggtagtga tgatacttta
    35881 tttgcattag ttgatggtac tgttaaattc gaaagaaaag acaagaagag aaaaaaagtt
    35941 agtatatatc cagtagcaat agctgagtaa tatacataaa aggagtgttg aggcactcct
    36001 ttttcttata atactgatga atttataatg gtatttttgg taatgctaat taaaagtttt
    36061 taattaagta ggtgaagaat ttgtttatag ataaggcaag aatatttgtt aaggctggaa
    36121 atggtggaaa tggttcggta gcatttagaa gagaaaaata cgttccagct ggtggtccag
    36181 acggtggaga tggtggtcgt ggagcaagca tcatatttga agttgatttg gggcttagaa
    36241 ctctaatgga ctttaagtac cagaaaaaat atcaagctca aaatggtggc gacggaagca
    36301 aaggaaaaag agctggtaaa aatggagaaa atttagtatt aaaagtacca gcaggtacag
    36361 taataagaga tgaggctaca ggtcttgtat tagcagattt aaaaaaagaa ggagatacag
    36421 ctatagttgc aaaaggtggt ataggtggta aaggtaacca acactttgca aatgcagtaa
    36481 gacaagctcc agcttttgca aaatctggta cagatggaga agaaagatgg ataactcttg
    36541 aacttaaaat gatagcagat gttggtcttt taggatttcc aaatgtaggt aaatcaacat
    36601 ttctatcagt tgtaactaaa gctaaaccca aaattgcaaa ctatcatttt acaacactta
    36661 caccaaatct aggtgttgtt caaactaagt ttggagatag ttttgtactt gcagatatac
    36721 ctggaattat agagggtgct tctgaaggca taggattagg tcatgagttc ttaagacatg
    36781 ttgagagaac aaaggtattg atacatattg tagatatatc tggattagag ggaagagacc
    36841 ctattgaaga ttttgacaag ataaatgatg agcttaaatt gtacaatgaa aagctttcaa
    36901 agagaccaca agttgtagtt gcaaataaat ttgatatatt agaagatgag agtaagtttg
    36961 agaagtttaa gagtgaatta gaaggcagag gatatacagt atttaaaatg tcagcagcaa
    37021 ctcgtcaagg catagatgaa gtaatagcct atgtttctaa aatgctaaaa gaagtagaag
    37081 atgtggagct agtttcagaa gaagaaatgt atagaccaga acttgatatt ggtactgaag
    37141 aagagttaag tatagatatt gaagatggtg tgtatgtagt tacaggtaaa gcacttagaa
    37201 gaataatgta ttctgtaaat tttgatgata tggaatccct tcaatatttc caaaaagcta
    37261 tggagagtca aggtgttttt gataggctta gagaaatggg aatagaagat ggagatgtgg
    37321 ttaaaattta tgaattagaa tttgagtttt acaattaatt taaatataga ttatagataa
    37381 tttaagtata gaattataga ggtgagaata tgttaaaagg aaaacaaagg gcgtatttaa
    37441 gatctttggc aaatacttta aagcctacta cacaaattgg taaagaaggt gttacagaga
    37501 gtttcttaga gcaattagat ggtatgttaa gaactagaga attagtaaaa gttactatat
    37561 tagaaaatgc aggtcttgat actaaagaaa ctgctaatgc agtatgtgaa gctttaagag
    37621 ctgaatttgt gcaggctatt ggttttaaat ttactattta caagaggaat attgaagagc
    37681 ctaaaatact ttttccagga catgaacagg cgaaagcaaa gactaaaaat aataatgtta
    37741 caaaaaaggg taaaccaact aaaagagctg ttagataata gtttatatcc ttttagggaa
    37801 ataagttaaa aaaatagtga caattggaag aaattgtatt atactattaa acataaattt
    37861 aaattagcaa tcttatcaag agtggcagag ggactggccc tgtgaagccc ggcaaccagt
    37921 atatttaatt atacattggt gctaaatcct gcagcattta tgctgaaaga tgagtataaa
    37981 cccgtcatat ggcttgagtt tatactcaag ctttttttat tggtatattt atatatataa
    38041 ataatttaat ataatcaaaa tgatgggggt atgttacagt gaagatagca atattagggt
    38101 atggaacagt aggaagtgga ttaattgatt taatagaaaa taatacttca aaaagagata
    38161 tagaagttgt tggaatttta gttaaaaata aagaaaaaca caagtataag aagtattttg
    38221 ataagataac tactgatata gaagatatat ttgacaagga tattgatata ctagttgaag
    38281 taattggagg attaaatcca gcttttgatt atgtaacaag agcgttaaat aaaaaaatac
    38341 atgtagtgac agctaataaa gatttactgg ctgaaaaagg tagtgacctt atagaattag
    38401 ctaacttaaa taatgttagc atcaagtttg aagcatctgt ggcaggtggt attccagttt
    38461 taaaaccatt gatagaatca ctggaaggca acaatataaa gagcataaac gctattttaa
    38521 atgggacttg taattttata ttatcaaaga tgtatgatga aaatttaccg tatgatgtcg
    38581 ctttaaaaca ggcacaagag ttgggatttg cagaagttaa tccagatgca gatgttttag
    38641 gatatgatag tgctagaaag ttatctatat tgtctacttt atcttatgga aaaatagtat
    38701 attggaaaga tttattatta gaaggaataa cttcaataga tgaaaaagat atagaatatg
    38761 caaaaaaatt aaattgtaag ataaaattgg ttgctagaag taagtatgaa tcaggagaag
    38821 taagtggatt tgttagacca gcattagtgg acaataataa tatgctttca aagatagata
    38881 atgagttcaa cgtagttatt ttagtaggtg attcagtagg tgaactttct tttgtaggta
    38941 agggagctgg aagaggtgca acaggaagtg ctgtttatgc agatattata gatataatag
    39001 ataatagaag ttcaaatata aagtcttttt caaaagggaa attggattta agtggactta
    39061 tagaagatga gtgtagtgca gtagttagat ttggtgggta taataataaa gatggaattt
    39121 tagaatgttt agataaatat gtagatagtt atgatattat agatgatgaa gaattggcga
    39181 tttttgtaag tgcaaagtca gaacatgaga ttgatagagt tttggaaatt gtaaaaagaa
    39241 ataattacag tgaatcagtt aaaaaattat tgaagataga ttaatatgtt tatgcaagaa
    39301 ttatgaaaat ttaatagggt gtctcaaaat agagagataa gccttttttc cagtgttgaa
    39361 ataataaaaa tatatttgat tttataaaaa aagagtgtct catgaactaa aaagttcatt
    39421 ttgagacact cttttttaat atctggtgaa ttaataagat aatagaaaaa tgtatacgtt
    39481 ataattaacc aataaagaaa ttttcactag cttcattaat agcttgctga gttaccctta
    39541 tatatcgctg tgtagtggct acagagctgt gatttaaaag ttctttaacc aactctaaat
    39601 tattgttact tttttcaaat tgagtagtag catagagctt tctaaagcta tgtgtactta
    39661 tattatacag atttaagtgt ttacaaatta tcctcaactg tttttgaata gcctttgtct
    39721 ttatatttac taagtagtca tttgatttaa gattatactt tcttgcatgt tcatttatgg
    39781 actcaattat atttttatta actggtctat actgtaattt gcccgtcttt ttttcattta
    39841 tttccaacat attaccttta aaattaccta tctttaatct taaaatatct gatattctta
    39901 gacctaaatt agcttctaac attagtgcca atgcaacttt aggatttttt ctaaatcttt
    39961 tttcaacgcc attttcacta taagtaaagc cagtatgtaa tagttccatt atctttttat
    40021 attcgtctat ttctataggt cttgttgcca ttttcaacca ctcctcttag tttgatttgg
    40081 taagtttagg tcggtattta ttatataata taaatgaaat attatataat ggaaaaattt
    40141 cccagtaaac ttatacaagt gtgatatatg tgattaagga gaattttttg aaaaatattt
    40201 ctaataaaat tattaaattg tgatgaaata actatgctgt taaactaatt aaaaaggtta
    40261 ttaaaattat gacaaatata ataaaaaaat ggaagtattt agttaagttt tattagtttt
    40321 atttttagtt ttattttttt cgtgaaaatt tcttagtgaa aaaagtattt ttatatcgca
    40381 ttagtttccc atttgtagat aaagttttca tatgtttttt tgcaataaat acggctgaga
    40441 ttagataaat tattactaca atttgaagaa ttgtatcata atagctaaga taataaaacc
    40501 agattaaatt attatatata acgttgtatg tatcagcaaa gctagaaacc aacacagcag
    40561 agtttgcaat tggataaagt ataaatataa agatagcagc taaaactcca tgcgcaaatt
    40621 tacttagtat gtataatttt aaatctatgt ccgtttttga tagaaatgta caacattgag
    40681 ctaggatgga taatccacta aatcctatca aaaaactagc taaagatgct cttaaaattt
    40741 ctggagaact tgttatacta gatactttat tgcatcctat agtaatttca aatagaccac
    40801 ttacaaatgc ttgacagagt tctttactaa cacctaaaaa tgaaagaggc aaatatataa
    40861 ctgatgctat taatgatatt acattaaaaa gagatagtat tttaaatact actgaaaaaa
    40921 caattacaaa tccaccaact gtaagtaatg tatttactcc attaaataca gcatttccaa
    40981 ataaaacaaa aaaaccattc ccacttgaat ttctaatgtt gattacattt tttatatttg
    41041 tttttaaatc caattttgtc tttggaaggt tttcattacc ataatttcta aagaaaagac
    41101 caaccaatat agttcctaga taatggcaga gaatcattaa ataacctaag tttgaatttt
    41161 ggaacatacc tgtaccaacc gaacctatta tgaataaagg tcctgatgta gaacaaaaag
    41221 acacaagtct ttgtgcttca tacttagaaa tttgtccatt actcctaaat tcagaagcta
    41281 aacttactcc tactggataa ccagatactg tagatatagc aaaagctaaa gcgctctttc
    41341 ctgaaacatt aaatacaaat ctagttatgg gattgatgat aaaccctata atatccacta
    41401 tttttaaatc aacaattaag tttgctccaa ttataaatgg tagtagtgat ggaactaaaa
    41461 cattagacca tatagaaaag ccttctcttg cagattttat agcttcattt gggtatagga
    41521 taatccctaa aactagaact ccaactatga tagatggagc aaaaaaagca ataattttat
    41581 tttttttcat aatatcacct gtaaatttga gttataagat attatattaa aagtaataaa
    41641 aatatatgaa tgtaatggct taagatatat aatagatata tattacaaca acgattatca
    41701 tattggagga aaaaatgttt aatagtatag ctagtaaaaa agtttatgaa caagtaattg
    41761 agcaaataca atataaaata ctaaatggag agttaaaaaa gggggataaa ttactatcag
    41821 aaagagaact gtctgaacaa atgaatgtga gtagaacttc cataagggaa gctattaggg
    41881 tattagagac tatgggagtg atagaaagca gacagggtga aggaaatttt atatgtacaa
    41941 atatagaaaa aactttaata gaacctttat ctatgatttt taaacttaat aatggtacac
    42001 ttgaagatat attagagctt agaataatcc tagaaattga gattgcaaaa cttgcttcaa
    42061 agagaataac ttcttctgaa gttatagaat tgaaacatat aatagatgaa atgagggtag
    42121 aaactaataa aaaagataat aatcgagtac tggtattatt agaccaaaaa tttcacagta
    42181 aactagcaac attaagtaaa aattatttaa ttcaaagtct ttttatgaca gcatcaaaat
    42241 tatttgatgg atttatagaa gatgcaagag aaaaaataat cgcagaacct tttaatgaaa
    42301 atatattgct taaacagcat gaagctatat acaatgcagt tgtagaaaat gatgtagaac
    42361 ttgcatgtga gaaagcaaag gaacatatgg attttataag taaaaattat agaaaaaatg
    42421 aaaattaaaa gtttgttata aaataaatta ttcttgcaaa tggtagataa tggtactatt
    42481 atataaatgt aaaactggta tgaccacata ccaattttat agtttattat aatgggagat
    42541 taaaaatggt aaagttaaaa gtaccgtatt caaaacaagg aatgatttta gaaatacctg
    42601 atgaacgttt tctcggtgtt ttggagtcaa aatcggataa ttatatagaa cataagactc
    42661 aagaagagat agttgaagaa tctatgaata atcctatagg ctctaattct ctggaagaac
    42721 tggttaaggg gaaaaagaat gtagtcataa ttacaagtga ccatacaaga cctgtaccaa
    42781 gtagaataac tatgccaatt atattaagaa ggattaggaa tgttaatcca gaaattgatg
    42841 tgaagattat agtggcaact ggatttcata ggccaagtac aagggaagag ctaatttata
    42901 aaatgggaga agaaatagta gataatgaag atatagttat gcatatatct actgatgata
    42961 aatctatgtg taaagtaggg atattgcctt caggtggaga tttatatata aacaaactag
    43021 catatgaggc agaacttttg atagcagaag gttttataga acctcatttc tttgcaggtt
    43081 tttcaggtgg cagaaaaagc gtacttccag gaatcgcatc tgcaaagact ataatgtaca
    43141 atcactgtag tgactttata gatagtgata attcaagaac tggaaaattg aataataatc
    43201 ctattcatga ggatatggta tatgcagcta aagttgcaaa attagcattc atattgaatg
    43261 ttgtaataga taaagataaa aaaataattg cttcctttgc tggtgatgtc gaaaaagcac
    43321 atactaaagg atgtagattt gtaacagagt tatcaaaagt aaattctata aaatctgata
    43381 ttgttgtaac tacaaatggt ggatatccac tagaccaaaa tatttatcaa tctgttaaag
    43441 gaatgacagc agctgaagct acttgtaaag acggtggagt aataataatg atttcagcat
    43501 gtaatgatgg tcatggaggt caatcttttt atgaaaatgt agcaaatgca gattctccaa
    43561 aacaactttt agataaaatt agaagtgtcg atagagtaga tacgattcca gaccaatggg
    43621 aatttcagat actagcgaga atattatcta aatatactgt gataatggta acagataagt
    43681 gtaatcctga aatgataaaa aaaatgcata tgaaacatgc atttaacttt aaagaagctt
    43741 tagaaatggc gaatagtata gttggtaagg agagcaaagt agtagttata cctgatggag
    43801 tatcagttat agttaaataa aaaaggagtg tactttatgg aaattttgac atgtattaaa
    43861 caagttcctg gaacgacttc agtagaagtt gatgaaacta caggagtact aaaaagagat
    43921 ggagtagatt caaaaatgaa tccctatgac ctatatgctt tagaaacagc tcttagaata
    43981 aaggaagata agaaagcgaa tctaaaagta cttagcatgg gtcctcctca ggcaaagaag
    44041 gtaattgaag aaagttttat gatgggagct gatgaaggag cattaataag tgataggaga
    44101 tttggaggag cagatgtatt ggctacttcc tataccatat cacaaggtat taagaagatg
    44161 ggaaaggtag acctaataat ttgtggaaaa caaactactg atggtgatac agcacaggtg
    44221 ggacctgaag tagctgaatt tttagatata cctcatgtta ctaacgttac aaaattaatt
    44281 gaagttaaag atgaaagtat agttgttgaa atagatatgc caaatgattt acaagtatgt
    44341 gagattgaat atccatgttt aataactgta gaaaaagaca tttttcaacc aagactacct
    44401 tcatttaaat taaaacttaa tactaaagat agagaaatac ctgtatatag cttagatgac
    44461 tttgaagata aaaatgaaaa taactatgga cttaatggtt ctcctactca agttgtaaga
    44521 atatttcctc caaagccaaa tacagataaa aatattgtaa gaggaaatgc agatgaacta
    44581 agttttgcac ttgtaaataa attagaggaa ttaaagttgg tgtaaggggg agtaataaat
    44641 gtcaaagata gttgtaaatc aagataaaat aactgactta aagagaattt tagaaatatg
    44701 tccttttggg gctatagaag aaaagtcagg tatagtagaa ataagtgctg gctgtaagat
    44761 gtgcaagttg tgtgtcaaaa gtggaccaaa aggcgcattt gaatttatag agagtagtaa
    44821 agtacaaatt aataaagatg aatggagggg aattgcagta tatgttgaac atcataatgg
    44881 aaatatacat cctgtaacgt acgagcttat aggtaaagct cgtgagatgg catcaaaaat
    44941 aaaacaacct gtgtattgtg tatttgtagg aaaagacata aaagataagt gcagtaattt
    45001 gatttcttat ggggtggatg aagtatttgt atatgatgaa gatgaattta aagattttag
    45061 gatagaacca tattcaaaag caatagaaaa ctttatagat aaaataaaac caacgatagt
    45121 attagttggt ggaactacat taggtaggtc attagctcca agacttgccg ctagatttag
    45181 aacaggtctt acagcagatt gtacaatact tgatattcaa tcaaatactg atttagacca
    45241 aataagacca gcatttggtg gaaatataat ggctcatata aacacaccta ataatagacc
    45301 acaatttgcc actgtaagat ataaaatatt ttcagcacca gaaaaaatag agaatactac
    45361 aggaaagata actttatgta aagtagaaaa aaaagaacta aaatctaaaa ttaaggtact
    45421 gagtgtaaaa gaaaaaaata aagaagttgg tctggaagaa gctgaagtga tagttgtggc
    45481 aagtagagca ataaaaaaac aagaagatat ggagatgata tataaattag ctgataagtt
    45541 aaacgctcaa gtggcaggaa caagacctgt aatagaagca ggttggatag atgctaaaaa
    45601 acaaataggt cttagtggta gaactgtaaa acctaaattg ataataactt gtggtgtatc
    45661 tggagctgtt caatttgttg cagggatgca gggtgctgac tatatagtag caataaataa
    45721 agatgataaa gcgcctatac ttgatgtagc acatttagct ttaataggtg atatttatga
    45781 tattattcct aaattgattg aaaaaataga aaataacaaa gataataatc aaaaatatat
    45841 ggcatcagca gctaaatagg gggatagaaa tgtataaatt aatcgataaa aaagatatag
    45901 attttttaat agatacttgt ggagaagaaa atgtattagt tggaagtgat ataaatgaag
    45961 atttttcaca tgatgaactt ggagggattg agaagtaccc tgaagtttta gtaaatgtac
    46021 ttgaaactga acaagtatct aaaataatga aatacgcata taaaaataat ataccagtca
    46081 caccaagagg tcaaggtact ggtcttgtag gtgctgctgt tgccataaat ggtggaataa
    46141 tgattaatct atgtaaaatg aataaaatac ttgaagttga ttatgaaaat ttaacattga
    46201 cagtagaacc aggtgtttta ttgatgacaa taggtcaata tgttcaagat agagatttat
    46261 tttacccacc tgacccaggt gaaaaaagtg ctacaattgc aggcaatata aacactaatg
    46321 caggtggtat gagagcagta aaatatggcg ttacaaggga ttatgtaaga ggtcttgaag
    46381 ttgtactacc aaatggagaa attataaatg taggtggaaa ggtagttaaa aatagttctg
    46441 gatatagtat aaaggattta ttagtgggct cagaaggtac tttaggaata gttactaaag
    46501 ctatattaaa attgcttcct ttaccaaaga aaagtataag tttgcttata ccatttccag
    46561 atttatcaat ggcaatagaa actgttccta aaataataaa atcaaaatca ataccaacgg
    46621 ctatagaatt tatggaaaga gatgtaatat tggcagcaga agaattttta ggtaaaaagt
    46681 ttccagataa tacatcagat gcttatcttt tacttacatt tgatggaaat agcactgaag
    46741 atatagaaaa agaatatgaa aaagtagcta atttatgttt agaaaatggt gcattagatg
    46801 tatttatatc agatacacaa gaaagaaacg attctatatg gtcagccaga ggtgcatttt
    46861 tagaagctat aaaggcatct acgacacaaa tggatgaatg tgatgtcgtt gttccaagag
    46921 ataagatagc agagtttata agatacactc acgagcttca agacaaatta aaaataagaa
    46981 taaaaagttt tggtcatgca ggagatggaa atctacacat atatatttta aaagatggta
    47041 tggatgataa tacatggaaa ataagattaa aagaaacttt tgactatatg tataaaaaat
    47101 ctagagaact tagtggtcaa gtttctggtg aacatggcat aggatatgct aaaaaagagt
    47161 acttacatga atcaaattca gatgcttata tgatgttaat taaaaatata aagttagctt
    47221 ttgaccctaa aaatatttta aatccaggga aaatatatta gaaatattaa tttaataata
    47281 tttacaaaac aaaaaatatt aaattagatt taaatataat attttaaact ttactttt
//
LOCUS       DAGSWI010000011        46987 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.011, whole genome shotgun sequence.
ACCESSION   DAGSWI010000011 DAGSWI010000000
VERSION     DAGSWI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.011"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(410..784)
                     /locus_tag="MJF80_000569"
     CDS             complement(410..784)
                     /locus_tag="MJF80_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0279247.1"
                     /translation="MEKQNLEDNERRQRLKDIRLSKKLKQKDFGKKLGLSLSQISSYE
                     TGHRKIPQRTINDICREFNVNEQWLLHGEGEMFVDITEDLETSEEIKTMIKKIFALED
                     EDREAIEKIIDNAYLKIKNEEL"
     gene            938..1114
                     /locus_tag="MJF80_000570"
     CDS             938..1114
                     /locus_tag="MJF80_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279248.1"
                     /translation="MNQEKEKANDLLLKLELLKKINPQEFGYISGRIDVNIEKLKQET
                     ERNKSKKIRYRKGA"
     gene            1118..1324
                     /locus_tag="MJF80_000571"
     CDS             1118..1324
                     /locus_tag="MJF80_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279249.1"
                     /translation="MTFNKNQIGTAVQLQDIRFDTKVDCIITKVEKNEILVMYYEKET
                     EEIAYKTLTKEDLILDDYKLKLLY"
     gene            1353..2075
                     /locus_tag="MJF80_000572"
     CDS             1353..2075
                     /locus_tag="MJF80_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0279250.1"
                     /translation="MDLLKDARKKEWFWLENDLVDREDLSVYEKMAYMVMARYSDDES
                     SCFPSLSLISKKMGCGITTVKKTINSLEEKNLIIKVHRKVKNKKENDTNVYYIMSLKG
                     KSSGDIGHEATDGRSPYDQQVGREATSKKTYNKKTYIKSNTTPQNEPKTDYLDLSFLD
                     LDIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIINGKGSRYKSHYRALLTWGNGD
                     ASKGIVPEVTKTKNPLSGFKEL"
     gene            2103..2288
                     /locus_tag="MJF80_000573"
     CDS             2103..2288
                     /locus_tag="MJF80_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279251.1"
                     /translation="MNEKEETVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFDIKSDI"
     gene            2314..2532
                     /locus_tag="MJF80_000574"
     CDS             2314..2532
                     /locus_tag="MJF80_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279252.1"
                     /translation="MKDEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(2551..2763)
                     /locus_tag="MJF80_000575"
     CDS             complement(2551..2763)
                     /locus_tag="MJF80_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279253.1"
                     /translation="MNMCKIKTLREKCSLIDDKVEFSGEYKTFPGFTGSKLLECECNK
                     NYICKIQIMTANSNCPLFKKARQLQY"
     gene            2926..3186
                     /locus_tag="MJF80_000576"
     CDS             2926..3186
                     /locus_tag="MJF80_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279254.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNREAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            complement(3179..3367)
                     /locus_tag="MJF80_000577"
     CDS             complement(3179..3367)
                     /locus_tag="MJF80_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279255.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            3505..3822
                     /locus_tag="MJF80_000578"
     CDS             3505..3822
                     /locus_tag="MJF80_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279256.1"
                     /translation="MERKKHELTDQDIQRLIEGLRTDPKIREMIFESKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            3844..5175
                     /locus_tag="MJF80_000579"
     CDS             3844..5175
                     /locus_tag="MJF80_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBZ0279257.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDVGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIVDINDLKALVTTSSSINGNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAEAIANKEKAFLYSG
                     ELPSFMAMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYAVELISDWIEDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRLFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVIDEEEEADDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQAEIPEDVF"
     gene            5549..5935
                     /locus_tag="MJF80_000580"
     CDS             5549..5935
                     /locus_tag="MJF80_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279258.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSASVIDFRNASSK"
     gene            5987..6691
                     /locus_tag="MJF80_000581"
     CDS             5987..6691
                     /locus_tag="MJF80_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279259.1"
                     /translation="MIRNAIEYLVKLGKRETVCVDGFKYTTDALEIIEEPKANELEIT
                     TLSGLVDYIKSGIDHKEDELLLIQVLTPRKVLLKSPLRKNRDRETYIECVALLPEKRF
                     DRPLDLDSFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEVEQPESEFIFRMSSGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            6749..6931
                     /locus_tag="MJF80_000582"
     CDS             6749..6931
                     /locus_tag="MJF80_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279260.1"
                     /translation="MKSISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            6928..7305
                     /gene="ssb"
                     /locus_tag="MJF80_000583"
     CDS             6928..7305
                     /gene="ssb"
                     /locus_tag="MJF80_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBZ0279261.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYAKKDGTR
                     EVDFIPIEVMGGSAEFCANYLTKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DHKPKDEEKEIGFQVLDDEDIPF"
     gene            7283..7867
                     /locus_tag="MJF80_000584"
     CDS             7283..7867
                     /locus_tag="MJF80_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBZ0279262.1"
                     /translation="MMKIFHFKLDGDYMRSLEEKNKLFEDNINLVHFIINRYFKSFLR
                     RYPYLKEDLFQEGYIGLYKTTCCFDESKGKFSTIAFSYISGHLKRFTTGYVKKHYRND
                     IDSFEKCIYRDNYGEEIRIEDRLAGNENVDIENVRVIRSCIKRSEIKDIQKIVCLREK
                     GYTQQEISKVLGTSQTSIYRRLKKLKTEINILGK"
     gene            7896..8054
                     /locus_tag="MJF80_000585"
     CDS             7896..8054
                     /locus_tag="MJF80_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279263.1"
                     /translation="MKTKIIGGRENECPICNGNIFKIETLIGIVWQCKDCGCMYQDMS
                     SKESKRGY"
     gene            8057..8383
                     /locus_tag="MJF80_000586"
     CDS             8057..8383
                     /locus_tag="MJF80_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279264.1"
                     /translation="MSCVIKCDFCGETIAEILNIKVFRIDDEGNIMSIGKDVCDKCYD
                     RLFRSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQECLGGCLEGRKKWL
                     ESEAMK"
     gene            8383..8919
                     /locus_tag="MJF80_000587"
     CDS             8383..8919
                     /locus_tag="MJF80_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HBZ0279265.1"
                     /translation="MKKTLIIVGVVISILIMFACLFLVTKNKAIDLEEQVQSATASVE
                     VQEKRRIDLIKNIVDCVKDYAKYENRTLKEVTDMRVSANDESIKEAKLNIQAVAEKYP
                     DLKANENYKQLMSELSITENMIAQHRENYNMQVRNYNKYIRKFPANLILNSMGYEKIE
                     SKYTEYKAKEDAPQNLFD"
     gene            8931..9737
                     /locus_tag="MJF80_000588"
     CDS             8931..9737
                     /locus_tag="MJF80_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279266.1"
                     /translation="MKFKTFEITKREFLVSIIIACMMIVLGFFIDDKVQSKFLEDNER
                     YYKATKINNDKDLFDYAMETSAGDSLISGRFKSVDSVSIEELKGDYWFIERTEEKYTM
                     HTRVVTTKDSKGHSHKYTETYWTWDYHDSIEKKCNKFNFMGKEFDAKVITNLSKERLA
                     LKKDIFKNNNLKIKGNYAYINNKRRYYYEVIPKEFNGTMYANLKNNTIANPADKNIKV
                     FKGESIQNVLENNNFNTGFFKVVFWIIWILITTIIIYVFYYLKNDWLEDK"
     gene            9762..10022
                     /locus_tag="MJF80_000589"
     CDS             9762..10022
                     /locus_tag="MJF80_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279267.1"
                     /translation="MKCKVYHAIKNIKDANELIDRFETENIFFELNNIDNENVLNGEM
                     DLTDIEDIASLQFILDGDLFIFSSCDEMIFMNKYTQRELGLQ"
     gene            10023..10664
                     /locus_tag="MJF80_000590"
     CDS             10023..10664
                     /locus_tag="MJF80_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBZ0279268.1"
                     /translation="MNKRIVCQDTNEIVYNYKEYQKTKHWESLKNEFYFSNECKCVLC
                     DSVKKVSLHHKTYRRIGAEELDDLIPLCNSCHYKVHRNNKQYLFNKATEKQIRCINDL
                     LEVYVDIVEELFMNEYIYLYVAMGIEFYNENLMLFEANHIINDLKFVSAMLYEFYATK
                     LEIKLPGYFNGFDYEEIKDSLYKKMQRLSKYIYVPKKQEIELLFKKRKKEHYE"
     gene            10719..11204
                     /locus_tag="MJF80_000591"
     CDS             10719..11204
                     /locus_tag="MJF80_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279269.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKNAIDTGITKDIIAEG
                     LDVCQTILTMFQVLEIENYITEGVETHNKKLNGRGWDLKEIGDDILNLIKNSMINELI
                     NMNKKLKCLDEKEFNAKIQFLDVDKLWNLCMPLVNLSTIKGVNESIDEMKMGEFLKNI
                     N"
     gene            11224..11481
                     /locus_tag="MJF80_000592"
     CDS             11224..11481
                     /locus_tag="MJF80_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279270.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPRE"
     gene            11590..12033
                     /locus_tag="MJF80_000593"
     CDS             11590..12033
                     /locus_tag="MJF80_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HBZ0279271.1"
                     /translation="MQSSKKDNLFSSAEGKLYNYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTSKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            12176..12721
                     /locus_tag="MJF80_000594"
     CDS             12176..12721
                     /locus_tag="MJF80_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279272.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDIIEEISSAAYVPDNLKYYDNVM
                     RETPGMYGRECSLDFIKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILDESIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFANVVE
                     DLKILVQDRQRKNYHGNKECF"
     gene            12725..13369
                     /locus_tag="MJF80_000595"
     CDS             12725..13369
                     /locus_tag="MJF80_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBZ0279273.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RIGDILALKVADVTDIKLDKKGKKIRVSKDWIRVIEEKRDYDREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAKKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            13584..14042
                     /locus_tag="MJF80_000596"
     CDS             13584..14042
                     /locus_tag="MJF80_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0279274.1"
                     /translation="MDVNEVEIIDSSYEKGKELTEDQCNMVTLLITGTTVTETARIIG
                     VNRKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVM
                     LAANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            14597..16336
                     /locus_tag="MJF80_000597"
     CDS             14597..16336
                     /locus_tag="MJF80_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279275.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLSKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            16337..16540
                     /locus_tag="MJF80_000598"
     CDS             16337..16540
                     /locus_tag="MJF80_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279276.1"
                     /translation="MGGNLLANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRA
                     RSKSYISKQDIPIKVLKFIESTG"
     gene            16553..17830
                     /locus_tag="MJF80_000599"
     CDS             16553..17830
                     /locus_tag="MJF80_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBZ0279277.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            17808..18569
                     /locus_tag="MJF80_000600"
     CDS             17808..18569
                     /locus_tag="MJF80_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBZ0279278.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            18628..18954
                     /locus_tag="MJF80_000601"
     CDS             18628..18954
                     /locus_tag="MJF80_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279279.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            18980..19582
                     /locus_tag="MJF80_000602"
     CDS             18980..19582
                     /locus_tag="MJF80_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBZ0279280.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            19611..20534
                     /locus_tag="MJF80_000603"
     CDS             19611..20534
                     /locus_tag="MJF80_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279281.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            20548..20820
                     /locus_tag="MJF80_000604"
     CDS             20548..20820
                     /locus_tag="MJF80_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279282.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            20804..21184
                     /locus_tag="MJF80_000605"
     CDS             20804..21184
                     /locus_tag="MJF80_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBZ0279283.1"
                     /translation="MSTMLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELS
                     IALEGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENS
                     ISSSSFLTDTDKKFLSPFCIVSMY"
     gene            21196..21543
                     /locus_tag="MJF80_000606"
     CDS             21196..21543
                     /locus_tag="MJF80_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279284.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITELERI"
     gene            21543..21899
                     /locus_tag="MJF80_000607"
     CDS             21543..21899
                     /locus_tag="MJF80_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBZ0279285.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVKTEKLKNGWEEKQPEYDTYIVKNEVDYATLVNNGHSTKNGGIVPGSFMVEKGIE
                     RTKENMTSAKANLFDW"
     gene            21908..22357
                     /locus_tag="MJF80_000608"
     CDS             21908..22357
                     /locus_tag="MJF80_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279286.1"
                     /translation="MLTNRDIIIAVNKRIENEFRYIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKVLDTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLIIE
                     NISPSIAKDPVGYKLTFLMTVSYFEEVYFENETFEIMESVDLEIGSD"
     gene            22358..23413
                     /locus_tag="MJF80_000609"
     CDS             22358..23413
                     /locus_tag="MJF80_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBZ0279287.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEIE
                     DIPENYSAANLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMLD
                     ETEQDLPKIKTWIKKMREDNKIKVKAIIASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDEGSTVEINLDAQRAWLKKQNIDISNMEEQQIKEYNTDTLVFLKA
                     RIKLIDAMEDVYLDISM"
     gene            23429..23869
                     /locus_tag="MJF80_000610"
     CDS             23429..23869
                     /locus_tag="MJF80_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBZ0279288.1"
                     /translation="MKAKIDAKRIISGTFGECWLDNKQLTSTKGMEAKVDIDKEEIKL
                     CGQIMTTYKMIGATGKGSITLYKISTLFGEYVEKMLNEGKEFKVTIISKLQDPDSYGS
                     ERIALYGCMFDDLTLADWEAAKVGEIEVPFTFEGFEYLDKIKEK"
     gene            23884..24300
                     /locus_tag="MJF80_000611"
     CDS             23884..24300
                     /locus_tag="MJF80_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBZ0279289.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSAFELYCKKLDDTLEIECKAIDPER
                     FDEIRMNSMDVNSGDVENINIYELKVNTILESCSIFSNMELVKKFSAITPKELIKKLL
                     LSGEIDKLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            24327..24476
                     /locus_tag="MJF80_000612"
     CDS             24327..24476
                     /locus_tag="MJF80_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279290.1"
                     /translation="MYLLFKYKGIMPSVSYRILRGEKEIVSVFMDQELDEREEEIKSG
                     AGRLF"
     gene            24480..27869
                     /locus_tag="MJF80_000613"
     CDS             24480..27869
                     /locus_tag="MJF80_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBZ0279291.1"
                     /translation="MSSNTKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRTLN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKDMTDKQKNYYLAAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGIFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPIGWTITAVIALSVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFNLIIGIVTGDSKKMMNG
                     VKQIFDGGVKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWKDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTIANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKECFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSFFKDKVKGVKEAWNKLKDFFKHPIKGTIEI
                     FKKGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKVGGVL"
     gene            27916..28392
                     /locus_tag="MJF80_000614"
     CDS             27916..28392
                     /locus_tag="MJF80_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBZ0279292.1"
                     /translation="MNKKIPALAILIVLSLSLVGCSTNDSKNGDSKPKQNTEEIKNHK
                     IGEVVDLDGAKVSVKNVRKLESDSNVYAVEIEITNTGDTDLESDFLQNYILKTDDNTV
                     GNAINNQELNGELLFDTIHPGDTLKGEIAFELTKDTKPKTIEIAYENVGKYTVFDL"
     gene            28450..29085
                     /locus_tag="MJF80_000615"
     CDS             28450..29085
                     /locus_tag="MJF80_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBZ0279293.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDVYFTLSLKEYRRIQISKVNINNDEKLSSEKDVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYGNGDLWEKIYKANEDKIKDPSVIKNGWILIIP"
     gene            29325..30842
                     /locus_tag="MJF80_000616"
     CDS             29325..30842
                     /locus_tag="MJF80_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBZ0279294.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFVDNKFPLGNLPKTNVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESMENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYSAEFTAYCPR
                     KSEGGDKDCRGKKLDPSKKTCAAPMVGTYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNVTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            30856..31188
                     /locus_tag="MJF80_000617"
     CDS             30856..31188
                     /locus_tag="MJF80_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBZ0279295.1"
                     /translation="MADPINEFIGIMRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGDTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            31181..31615
                     /locus_tag="MJF80_000618"
     CDS             31181..31615
                     /locus_tag="MJF80_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBZ0279296.1"
                     /translation="MNNTIFPFMGVPEDYISPTNEELPIFKEFAWDFNKDEKIIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVHLIYDWDYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     ETLLTNPYILEVNVTNTKFKDDNLSISLNVKTIYGEEEITFA"
     gene            31608..32660
                     /locus_tag="MJF80_000619"
     CDS             31608..32660
                     /locus_tag="MJF80_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBZ0279297.1"
                     /translation="MHNGQSFSVLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVIFEGKIGTKIPNGTII
                     SYSDLLFVVIKDITIDEDSKLNVSPIQALEVGIKYNLSAKTEFKLIEEINGVTKIYNE
                     LDIKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCTEHIEEEKPIGPTITIVTPSTFDITISATLTLENGY
                     DIESIKVMFLDIINSYLIENSKEIIYIKIMSLLASIEGVHDIKNLLVNDDVKNIIVDE
                     EKVPAVSSAIFDIEVS"
     gene            32662..33279
                     /locus_tag="MJF80_000620"
     CDS             32662..33279
                     /locus_tag="MJF80_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBZ0279298.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLDQFFID
                     TATWGLDDWESLLDIKINYKLDFDTRRSNIKAKLRGKGTTTIEVIKAISEAYTKTNVD
                     VKVFSNLFSFTLSFLTNDCSYNTILELDKKIEEIKPCHLEHKFEMILFNKNNMFTGST
                     MNTGETVTIYPYSTTDITSFGELTITTGNDRSMEKVTLYPRQEVV"
     gene            33281..34105
                     /locus_tag="MJF80_000621"
     CDS             33281..34105
                     /locus_tag="MJF80_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBZ0279299.1"
                     /translation="MAEQKYYTLLTTIGKASIANATALGNKVDLVKLQLGDGAGAEYN
                     PTEEQTSLKNVVWEGSINNVKIDEENPNWIVIETVIPGSVGGFMIREVGIFDSKEQLI
                     AVSKYPETYKPTADSGSVKDLVIKIILVVSNTSSVNLKVDPTVILATLKDIQDLDSKI
                     DTTKTELTSNIETAKTELNTKIGDTTQLTTTDKTNIVNALNEVKTSVDSIETTAEKTS
                     IKDIDNLFESDNVEGALNQLAINYNILLEKQDNLETEVNGQRIKGISIANSLIDMI"
     gene            34119..34715
                     /locus_tag="MJF80_000622"
     CDS             34119..34715
                     /locus_tag="MJF80_000622"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279300.1"
                     /translation="MTEKLTDNASLRELMIAFESAKNDLQIDKNNIASTLGSPFLGTD
                     KLDTTKTKTQTLKNTLASNITNKGIVTNSTESLQDMISKVSSIKDLRQASGTASAFKG
                     RFYDDYYYLWSVNLPQLNFVPLVVFVYADYRRLSIWVKSPFSKYIFYGKKDNVKTELK
                     DFYIKELDDGISTETVIHLYGASGDTYEVQWLAIGQEA"
     gene            34718..35011
                     /locus_tag="MJF80_000623"
     CDS             34718..35011
                     /locus_tag="MJF80_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279301.1"
                     /translation="MEVGKLIIYDNKGKILFQGGEGKGDLIPYDKDMKVDWVEIKYNS
                     IDYSKYRIIGINVETKEPVLEEIKVELTEEEKRIQELENQILIAENEKVGGIL"
     gene            35011..35193
                     /locus_tag="MJF80_000624"
     CDS             35011..35193
                     /locus_tag="MJF80_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279302.1"
                     /translation="MNINNVVVRILAERILSGGLNPLKNREFELDDVTNAEYRKAVED
                     YIIRESGVIEGTELTV"
     gene            35233..35463
                     /locus_tag="MJF80_000625"
     CDS             35233..35463
                     /locus_tag="MJF80_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBZ0279303.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQRGIF"
     gene            35479..35736
                     /locus_tag="MJF80_000626"
     CDS             35479..35736
                     /locus_tag="MJF80_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBZ0279304.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVVLLAL
                     GIGFSIWMLGLNPVAVLQGVICWGISIGINQTYKQLKEENK"
     gene            35736..36539
                     /locus_tag="MJF80_000627"
     CDS             35736..36539
                     /locus_tag="MJF80_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBZ0279305.1"
                     /translation="MKIAIVPGHTLSGKGTGTTGYIDEGKENRILTDLIVKWLKQGGT
                     TVYTGKVDKSNNYLAEQCQIANKQDIDLAVQIHFNANKTTLNAMGTETIYKTNNGKVY
                     AERVNKKLSTIFKNRGAKSDARGLYWLKHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKNINNKENGEGKIMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIK
                     DYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFIK"
     gene            36598..37367
                     /locus_tag="MJF80_000628"
                     /pseudo
     CDS             36598..37367
                     /locus_tag="MJF80_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894430.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(37796..38311)
                     /locus_tag="MJF80_000629"
     CDS             complement(37796..38311)
                     /locus_tag="MJF80_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279306.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRVGNRFLEDNKSYDDKYHIDSINSKIDDDIKYLSNEIDNIKSVIEWFKTK
                     DDKCHIDSIKENDISIDLPDQSIKRTTIRVNNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(38394..39299)
                     /locus_tag="MJF80_000630"
     CDS             complement(38394..39299)
                     /locus_tag="MJF80_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBZ0279307.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEVIDLALGLPVKQFGGLRKDLIEK
                     LQGKEYHVEFAKGNETTKRDITIRSVQVFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSVNVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYIDK
                     GFYLNGEKQDIKKNIDASVGLFKQIYNELKLNYPISTSAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            complement(39318..39521)
                     /locus_tag="MJF80_000631"
     CDS             complement(39318..39521)
                     /locus_tag="MJF80_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279308.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMNEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
     gene            complement(39771..40229)
                     /locus_tag="MJF80_000632"
     CDS             complement(39771..40229)
                     /locus_tag="MJF80_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279309.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSVVVPGGTANLTAMSGGQLHWRVTSTLGNILGFEGNIDVYKAGKIVSSQFVS
                     FRLAGTTKTGVVSFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            complement(40264..40566)
                     /locus_tag="MJF80_000633"
     CDS             complement(40264..40566)
                     /locus_tag="MJF80_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279310.1"
                     /translation="MEVNLHTGKDEFFKNHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            complement(40954..41106)
                     /locus_tag="MJF80_000634"
     CDS             complement(40954..41106)
                     /locus_tag="MJF80_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279311.1"
                     /translation="MDNFLFNVLASLTASVVVYLISELFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            complement(41226..41960)
                     /locus_tag="MJF80_000635"
     CDS             complement(41226..41960)
                     /locus_tag="MJF80_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBZ0279312.1"
                     /translation="MYYVYKYINQDTEECLYVGKTENICDRHASHLSNKKENWCNKNL
                     RLEYMELDNKYTMDFYEIYLINKLEPKFNITGKGEMDIAKTSFSYNGQWVIYLERDLM
                     SSLASKRYGINYEVNAKMLGIMRKLKKISSNEEIFIGNENIRIKYYFETVLEGINLEP
                     CILSVAYKTLKESTVGTVISVKREYLSENVCLIIDSIFLNEIMKDPLMSKSDIKDLNS
                     QVNDILKIIGVDCEWNKLSDYCSANS"
     gene            complement(41980..42780)
                     /locus_tag="MJF80_000636"
     CDS             complement(41980..42780)
                     /locus_tag="MJF80_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBZ0279313.1"
                     /translation="MYYVYKYIDPATEECLYVGKTEDIYARHSSHLSNKEEDWCTKEL
                     ILKYIELPRRYNMDFFEVYLINELNPIFNKLSKGDMDTEHIFFDYKEEDWSTYSEEDF
                     KEKTKGKRRFRERKIDMKYEVSKKSINFLKRIEKLDAKVEMLYENNVLSVLYNFENMD
                     IEFCENELDINLISYDFETLERFGSCSLISVYPRWKEYTATNKKILINITLEIRIGSL
                     ANLMELMPTFEYKITEIFQQIEDIFHILEVGYTWQELFEIYKGCKYDN"
     gene            complement(42800..43987)
                     /locus_tag="MJF80_000637"
     CDS             complement(42800..43987)
                     /locus_tag="MJF80_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBZ0279314.1"
                     /translation="MEKKEILMQPNNLIKSKYDFTNVENKLFYKILFNAQKQQNSSYV
                     TTISKEELKVFMKNNNDYEHKNIKEILNMFQQSVLEFDYIEETTGKLKTFGSGLINTY
                     ELDHTDQIYTIMMHEVLYNHITDFVKMQKKKNGYTAINLSVLFNFRGAYTQRLYTLFR
                     LWSRENKEVEIKYKLDELRFYLKLKDNVYPEYKYFKQNVLKRAMNEINKKGNMVVSIK
                     EEKRKNRKIDEIIFSVIDYEPRKYFDKDILVEDYIPKKKQDNEVSFSFYIPNENIFTT
                     GTIKLFKEDFKEFDFKKDVYLNAFNKSIAIAFERDNTDIINTKNYDFFKSTLSNKISE
                     VIIHQKDDLKFKEELDKYWDDEDVKKEEREVDYSKDPVRLETVRRNLFDQGLVNEEGY
                     YYK"
     gene            44407..45096
                     /locus_tag="MJF80_000638"
     CDS             44407..45096
                     /locus_tag="MJF80_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBZ0279315.1"
                     /translation="MLENRKQIQKEIYLPIATYCLKDKQYYIKEYGALLIKSAENSDI
                     FNTHKNRILYKNKLDIQEIANELNVNKATIQRNIKKLEKLDFKILKIENTLNGIVYCL
                     PSENNIDLNKFALINYEMLKKMVNKFKSNTIKVYFLLKTITTETNFKPATNSFIAENI
                     GLSSKSKNNLDIITSSVKTLEENKYIETKKVNVYEYDKEKLREVPKIRKVYRICNFDE
                     WKKEIDKNKVY"
     gene            complement(45245..45628)
                     /locus_tag="MJF80_000639"
     CDS             complement(45245..45628)
                     /locus_tag="MJF80_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0279316.1"
                     /translation="MKIKKIPESELLVMMYIWDSEDEEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHKNSFESFISAL
                     HDDEEVSENKLKSLEKFIEDWEEDK"
     gene            46214..46369
                     /locus_tag="MJF80_000640"
     CDS             46214..46369
                     /locus_tag="MJF80_000640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279317.1"
                     /translation="MGELKLESKMNCTKNKKKVAISIHQMKKISKKDYVFLTDCIETF
                     YMKIKEV"
     gene            complement(46395..46763)
                     /locus_tag="MJF80_000641"
     CDS             complement(46395..46763)
                     /locus_tag="MJF80_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0279318.1"
                     /translation="MAEVSKKLKAKEIGKRLNELRTLERLSQRAFGERIFLSQDQISL
                     LEKGKRVLTERSINDICREFEVNEEWLRSGKGEIYKDCLADLEIDDDVKEITNKLYEL
                     EQEDRDAILKMIELLQKKNK"
BASE COUNT        18851 a         5495 c         7664 g        14977 t
ORIGIN      
        1 aaaaaaaaat gaaccaactg aatggagaac tatagaaacc ataatctcca gtacatacgc
       61 attaagtaat ttcaaaaaag aaaataaaga cattatgaat cagaaataac aacagtaaat
      121 acttaagggg agtgataagc ttgaaaattt cagattttaa taaagatcat ataggtaaag
      181 cgattcaact acaagagaca agatttaatg tcataacgga ttgcatcatc acagaagtag
      241 aagtaggcga aattgttgta atgttttaca tgcaagaaaa tgatgacaca ggatacaagg
      301 ctataacatg ggaagattta aaaaattata tcataaatgg caaagggaag aggcattata
      361 ggatttttgt ggcttggggg attgttataa aaagaggtat aacctctttt tataactctt
      421 catttttgat ttttagatat gcattgtcaa ttattttttc tatcgcttct ctatcttcat
      481 cttccaatgc aaatatcttt tttatcatag tttttatttc ttcactagtt tctaaatctt
      541 ctgtaatgtc aacgaacatt tcaccttctc catgcaataa ccattgttca tttacattaa
      601 attctctaca aatatcattt atggttcttt gtggtatctt tctatgccct gtttcatatg
      661 aagagatttg cgaaagagac aaacctaatt ttttgccaaa atctttttgt tttaatttct
      721 ttgaaagcct tatatcttta agtcgttgtc ttctttcatt atcttctaag ttttgttttt
      781 ccataacgtc accccttatg agtataatta taacatttaa aaatacgctt tgcaattaca
      841 aattttaaaa aaatacgcaa aataaaataa aaactcaaag cgtattgaca tagtatgcaa
      901 agcgtaatat aattgaatca caggaaggag tgttagaatg aatcaagaaa aagaaaaagc
      961 gaatgatttg ttattaaaac ttgaacttct taaaaagata aacccacaag agtttggata
     1021 cataagcgga agaatagacg taaatattga gaagttaaaa caagaaaccg aaagaaataa
     1081 aagcaaaaaa ataagatata ggaagggggc ttaaaaattg acttttaata aaaatcaaat
     1141 aggaacagca gttcaattac aagatataag gtttgataca aaagttgatt gcatcattac
     1201 aaaagtagaa aagaatgaaa tactagtcat gtattatgaa aaagaaacag aggaaatagc
     1261 atataaaact ttgactaaag aggatctaat acttgatgat tacaaactta agttattata
     1321 ttaaaaagta atttcggaag ggaggtgatt aaatggatct tttgaaagat gcaagaaaaa
     1381 aagaatggtt ttggctggaa aatgatttgg tggatagaga ggatctaagt gtttacgaaa
     1441 aaatggctta tatggtaatg gcaagatatt cagatgatga aagttcttgt ttcccaagtt
     1501 tatcattaat atccaagaaa atgggatgcg gaataacgac tgtcaaaaaa acaataaata
     1561 gtttagaaga aaaaaattta ataataaaag tacatcgcaa ggttaaaaac aaaaaggaaa
     1621 atgatacaaa tgtttattac attatgagtt tgaaaggtaa gtcgtctggc gacataggtc
     1681 atgaggcgac agacggtagg tcgccatacg accaacaggt aggtcgcgag gcgactagta
     1741 aaaagactta taataaaaag acttatataa aaagtaatac cactccacaa aatgaaccta
     1801 aaactgatta tttagaccta tcatttttag atttagatat agaaaaagta aaactaacta
     1861 aagatgaata tgacaaactt ataagcaagt ttgggaagaa atacatacat gataaaattg
     1921 ttagtttaga aaactatatc ataaatggta aagggagtag gtataagagc cactataggg
     1981 ctttgctgac ttgggggaat ggagatgcta gtaaaggtat agtaccagaa gttacaaaga
     2041 ctaagaatcc tcttagtgga tttaaagaac tttaaaaact aaatagtaag gaggtagata
     2101 ggatgaatga aaaagaagag actgtttatt tgaatagttc taatgtgaag ttattcttag
     2161 gcgttaggga tattaaggaa tttgaggaat tgatagaaaa tgtcaatgag cgaatttgtc
     2221 aactccaaga atcgattaat aaacttgccc aatttaatat tgagttcgat attaagtcag
     2281 atatttagtt aagtataaat taggaggttg cctatgaaag atgaaaagtt agaacttact
     2341 gaaaaagatt tacactgtat tgcaagacat ttacaaaatg aagttatgga gatggtgttt
     2401 agagggaata tagaagcccc tacatcatgc gaagtttgcg attacttgca agaatgcaag
     2461 gactatttca cccatataga cacttttata aaattaagtg aaatgacagg tgtagatatt
     2521 tttactaaat aaatttagta aattaaaatt ttagtattgt aattgacgtg cttttttaaa
     2581 aagaggacag ttgctattag cagtcattat ttgaattttg caaatgtaat ttttattaca
     2641 ttcgcattct aaaagttttg atccagtgaa tccaggaaaa gttttatatt caccagaaaa
     2701 ttcaacttta tcgtcaataa gagagcattt ttctcttaag gttttaattt tgcacatatt
     2761 catcaccacc ctataattga tttgaacata ctgtcaatat gctccaatta attataacat
     2821 gcaaattatc atggtgtaga attaaagcat aggtcaatta aatttagtta taaaatcaat
     2881 gaaacagata aagaattaaa tgttaaataa attagggggt tgttcatgaa agaggaaaag
     2941 ttagaactta ctgaaaaaga attgcattgt attgcaagac atttgcaaaa tgaagttatg
     3001 gagatggtgt ttagagggaa tagagaagcc cccacatcat gtgaagtttg cgattacctg
     3061 caagaatgtg agggagattt tacctgtatg tatcattctt ttactaaatt aagcaaaata
     3121 acaggtgtaa aagtgactgc atttaagtat tgttttccag atgaaaatcc caaagctact
     3181 atctaagaag tttttttaga ataggacatt cttcattaga agaagagtcg caaatatgac
     3241 ttccatcaca tctagttgac attaacttag atccaaaagt acctggagga gctgaatatt
     3301 cgcaagtaag agaaatggtt tcgtttatta caggacattc tttgcttata actcgatatt
     3361 ggctcatact ctcaccacct ttctaattaa tttaaacata ttggaagtat gtttaaatta
     3421 attatagcat gtaaattatc atggtgtaga atagtttcaa atcaaaagaa gcaactaaat
     3481 agttaaagac aggaggtatg ctaagtggaa agaaaaaaac atgaacttac agatcaagat
     3541 atacaaagac ttattgaagg attaagaact gatccaaaaa taagagaaat gatatttgaa
     3601 agtaaaggtt gtgaaactag aacaagtaca agaagtaaat gcgaacaatt tgttgaagaa
     3661 aaacttgctt atagaagtta tggatttgaa actccaaaat actatgcaaa acttattgcc
     3721 gacataattg atattttagt ttacaaaaat gttccaagag atcgtatttg tggggtattg
     3781 tatgacgtcg aaagggtgat tcctttagtt gtgacatttt aataataagc tgggggtgat
     3841 taaatgaatt actctttaga agcagagcaa aacattctag gaagttttct tataaatgac
     3901 tctataggtt acaaaataag ggaattaaaa gaaaatgatt tctactttga gtcacataaa
     3961 attattttat cttgcatgaa gaaaataatt gactcaaata aaccactaga tcttttgctt
     4021 ttaaaaaatg aattagaaaa aatagataga ttagtagacg ttggaggagt tagttacatt
     4081 accagtttaa caactatagt cataactact tcaaacatag atcactatat aaaaatcata
     4141 aaagagaagt tacttaaaag acaaatttca gagctagcaa atgaattaat taataattca
     4201 aattcagata caagtataga tgaattgatt gtagatataa acgatttaaa agcacttgtg
     4261 acgacgagca gtagtataaa tggtaattat attgacgcat caaaaataaa acgtgaaaaa
     4321 ggcgtacaca agtccataga cacgggtttt aataaactaa acaatatgct agatggattt
     4381 agatatggaa ctcttacaat tttgactggt aaacctgcat ctggtaaatc aactatagtt
     4441 aaccagttta tagcagaagc aattgcaaat aaagaaaaag catttttgta ttctggagaa
     4501 ttaccctctt tcatggctat ggattggttt agaaaaactg tagctaatga ttatcacata
     4561 aaagaatata aaagtgtcta tggaggaaca tacacagata ttccagatta cgccgttgag
     4621 cttatatcag actggataga agataaattt tttctctatg atgaagatgc aataagtgat
     4681 gaagtaaatc tactaaatac catagaacat ttatatttaa aaaaaggggt taggctcttt
     4741 gttcttgaca atctaatgac aataaaaact ggcaataagg cagataaata tgagagacaa
     4801 gagcagatag taagcaactt aaaaaatctc gctaaaaaat ataacttagt aatagtttta
     4861 gttgcacatc ctagaaaaaa tatgggtgac atgaaaccaa ctatgtatga tgtgtctgga
     4921 gcaagtgaga ttgttaatta tgcagattat attttatcaa cttacagagt tattgatgaa
     4981 gaagaagagg cagacgacac ttatttatta attttaaaaa atagaataac aggaaaacag
     5041 aatataagtt tcaaaatgaa tttttcagaa agaagaaaaa gactttacac aactagtgaa
     5101 gagttaaata gagattacaa atatgatgta aataaacaat atgtgcaagc agaaattcca
     5161 gaagatgtat tttaaaaaag gataaaaaat aggaaatttt attaagtaat gattttacaa
     5221 taaaaatcaa tattgaataa tttaaaaaat caatattgaa aaaataccgt ttttcaatat
     5281 tgaaattaaa atttcataag gtattgaaaa tcaatattga aaaaaattaa taaatatagt
     5341 aattgcaatg attgcagaat acgattacat tacacaaaaa acacaggttt aaagaatttg
     5401 tcaaaaacta ttaaaagatg gtaatgatag agagaaaaaa ataaaatcaa aaattttgat
     5461 aaaaatgtta ttaaattata gtcaaagttt attaattaat ttcctcgtag aaaaatatta
     5521 ttttaaataa ataaaggagt gattttacat gatagcatta gaaaaatttg caggtggagt
     5581 tctaaaagaa aagtttaata cagagttaca aaaagtttta gataatattg cagatccaaa
     5641 tacagatttt aaaaaaacaa gaaaaattag tttagagata gtgtttaaag caaatgaaga
     5701 tagagatctt gctgaagtag atataaaatc taaagcaact atagtagaag caaaggctac
     5761 aacaactaaa gttctgatag gaaaagattt agagacagga agagttgaag cgtctgaatt
     5821 taaaaatcaa gtagcagggc agttggtcat ggatattcca gatagtgaag aaactaatga
     5881 aacagaagaa aatagtgcta gtgtaattga tttcagaaat gcaagttcaa agtagtttaa
     5941 gaaagtaagt aaaatataaa tttaaataaa aaaggagaat aaaaaaatga taagaaatgc
     6001 aatagaatat ttagttaagt taggaaaaag agaaacagta tgtgtagatg ggtttaagta
     6061 cacaacagat gcattagaaa taatagaaga accaaaagct aatgaattag aaataactac
     6121 attgagtggt ttagtagatt atataaaaag tggcatagat cataaagaag atgaattgtt
     6181 attaatacaa gttctaactc caagaaaagt gcttttaaaa tctcctttga gaaaaaatag
     6241 agatagagag acatatattg aatgtgttgc actactacca gagaaaagat ttgacagacc
     6301 tttagactta gatagtttta acataatgct acaatcagca tttataaaga ataatgatag
     6361 agatacactt ttaaaaattg caggtaatat ccaagaatct acagtaaaaa acgtgggaga
     6421 tgatggagtt tctcaatctg taacaataaa aacaggtgtc gctagtgtat cagaagcaat
     6481 agttccaaat agagtaaaat tgattcctta ccgtactttc caagaagtag agcaaccaga
     6541 aagtgaattt attttcagaa tgagttctgg accagcagca ggattatatg aagcagatgg
     6601 tggagcatgg aaaaatcaag caatgttaaa tattaaagcg tacttgcaag aagaattaaa
     6661 agattttaaa aatgtaaata taattgctta attgaaaatc taagaggact tattataagt
     6721 cctcttagta aaacaaggag ggagttaaat gaaaagtatt tctcatatga acattttaga
     6781 aagagcagaa tttacagaca aaattgctaa tgcaattatg tctgcaaata aagaaattga
     6841 aagaggttgt gcctttaatg aagcagttga gattgtaaaa caaatggaat atagagaaaa
     6901 taaatacaat gaaggagata ttgcaatatg aataatgtag ttttagttgg aagattagca
     6961 agagatccag aattaagata tataccagag tatggtactc cagttgctac ttttgcattg
     7021 gcagttgata ggggttatgc aaaaaaggat ggaactagag aagttgattt tataccaatt
     7081 gaagttatgg gaggatcagc agaattttgt gctaattatc tcacaaaagg aagaatggtt
     7141 tcaattcaag gccaaataag gatagaaaaa tatgaaaaag atggagagaa aaaaactttc
     7201 acaaaagtaa gaacaaaaat tgttaatgcg cttgatcaca agccaaagga tgaagaaaaa
     7261 gaaataggct ttcaagtttt agatgatgaa gatattccat tttaaattgg atggtgatta
     7321 tatgagaagt ttagaagaga aaaataaatt atttgaagat aatataaatc ttgtacattt
     7381 tattataaat agatatttca aatccttttt aagaaggtat ccatatttaa aagaagattt
     7441 atttcaagaa ggatatatag gattatataa aacaacatgt tgctttgatg aaagtaaagg
     7501 aaaattttct acaatagcat tttcgtatat atcaggacat ttgaaaagat ttacgactgg
     7561 atatgttaag aaacattata gaaatgatat agatagtttt gaaaagtgta tttatagaga
     7621 taattatgga gaagagataa gaatagaaga tagattggct ggcaatgaaa atgtagatat
     7681 agaaaatgtt cgtgttatta gatcttgtat aaaaagaagt gagataaaag atatacagaa
     7741 aatagtttgt ctaagagaaa aaggttatac tcaacaagaa ataagtaaag tgcttggaac
     7801 tagccaaacg tcaatatata ggagacttaa aaaattaaaa actgaaatta atatactagg
     7861 taaataataa atgatttaag tttattggag gtaaaatgaa aactaaaata attggtggta
     7921 gggaaaatga gtgtccaatt tgtaatggta atatatttaa gattgaaaca ttaattggaa
     7981 tagtgtggca atgtaaggat tgtggatgta tgtatcaaga tatgagttct aaagaaagta
     8041 aaagggggta ttaaagatga gttgtgttat aaagtgtgat ttttgtggtg aaacaatagc
     8101 agaaattcta aacataaagg tattcagaat tgatgatgaa ggtaatataa tgagtatcgg
     8161 aaaagatgta tgtgacaaat gttatgacag attatttaga agtaacttga ataagaagat
     8221 gactaattat gaaaagataa aaaatatgag caaaatagaa atggctgaat ttcttgcaaa
     8281 tggcggaagt ggatgtacta gttgtgctta tgattttcaa gaatgcttag gcggatgttt
     8341 agaaggtaga aaaaaatggc ttgaaagtga ggcgatgaaa taatgaaaaa gacgttaata
     8401 atcgtaggag tagttatttc aatattaatt atgtttgctt gcttgttctt agtaactaaa
     8461 aataaagcaa ttgatttaga agagcaggtc caatcagcaa ctgcatctgt agaggtgcaa
     8521 gaaaaaagga gaattgattt aattaagaac atagtagact gcgtaaaaga ctatgctaaa
     8581 tatgaaaata gaacattaaa agaagtaact gacatgagag taagtgcaaa tgatgaatct
     8641 atcaaggaag caaaattaaa tatacaagca gtagcagaga agtacccaga tttaaaagct
     8701 aatgaaaatt ataagcaact aatgagtgag ttgagtataa cagaaaatat gattgcacaa
     8761 catagagaaa attataatat gcaagttcgc aattacaata aatatataag aaaatttcca
     8821 gcaaatttaa ttctaaatag tatgggatat gaaaaaatag aatctaaata tactgaatat
     8881 aaagcaaaag aggatgcacc tcaaaattta tttgattaag aggtgcacat atgaaattta
     8941 aaacttttga aataactaag agagaatttt tagtgagcat aataattgct tgcatgatga
     9001 ttgtgcttgg atttttcatt gatgataaag ttcaatcaaa atttttagaa gataatgaaa
     9061 gatattacaa agcaactaaa ataaataatg acaaagatct ctttgattat gctatggaaa
     9121 caagtgcggg agattctttg ataagtggaa gatttaaaag tgtagattct gtatctatag
     9181 aagaattaaa aggggactat tggtttatag aaagaacgga agaaaaatac actatgcata
     9241 ctagagtggt taccactaaa gacagtaaag gccattcaca taaatatact gaaacatatt
     9301 ggacttggga ttaccatgat tcaatagaga aaaaatgtaa taaatttaat ttcatgggta
     9361 aagagtttga tgcgaaagta attactaatt tatctaaaga gagattggca ttaaagaaag
     9421 atatatttaa aaataataat cttaaaataa aaggtaatta tgcttacatt aataataaga
     9481 gaaggtacta ttatgaagtt ataccgaaag aatttaatgg tactatgtat gctaatttaa
     9541 aaaataatac tatagcgaat ccagctgata aaaacataaa agtttttaag ggtgagtcta
     9601 tacagaatgt tttagaaaat aataatttta atacagggtt ttttaaagta gtgttttgga
     9661 tcatctggat attaataact acaatcatta tatatgtatt ttattattta aaaaatgatt
     9721 ggcttgaaga taaataaaga tgtaagagga gaagatcagg tatgaaatgt aaggtgtacc
     9781 atgctattaa aaatataaaa gatgcaaatg agttaattga tagatttgaa acggaaaata
     9841 tattctttga attaaataac atagataatg aaaatgtctt aaatggagaa atggatttaa
     9901 ctgatataga agatattgca tcattgcaat ttatattaga tggagattta tttatattta
     9961 gtagttgtga tgaaatgata tttatgaata aatacactca aagggagttg ggacttcaat
    10021 aaatgaataa gagaatagta tgtcaagata caaacgagat tgtctataat tataaagaat
    10081 atcaaaaaac taaacactgg gaatcattaa aaaatgaatt ctatttttct aatgaatgta
    10141 aatgtgtttt gtgtgattca gtaaagaaag tgtctttgca tcataaaaca tatcgaagaa
    10201 taggagcgga agaattggat gatttaattc cattatgcaa tagttgtcac tataaagttc
    10261 atcgaaataa taaacaatat ctatttaata aggcaacgga aaaacagata agatgcataa
    10321 atgatttatt ggaagtttat gtagatattg tggaagaatt atttatgaat gaatatattt
    10381 atctttatgt tgccatggga atagaatttt acaatgaaaa tctaatgtta tttgaagcaa
    10441 atcatattat caatgatctt aagtttgttt ctgcaatgct gtatgagttt tatgccacaa
    10501 agttggaaat aaaacttcca ggatatttca atggatttga ctacgaagaa ataaaagatt
    10561 ctttatataa aaagatgcag cgattaagta aatatattta tgttcccaaa aaacaagaga
    10621 ttgagttatt atttaaaaaa agaaaaaagg agcattatga ataaatatac tcaaaaagag
    10681 ttgggacttc aataaatgaa tgaggggaga tactaaagat ggatttaaag ttaattagca
    10741 gcatacctaa ttggaaggta gaagatcagt tggcaaaaat tgatgaagag gtaactgagt
    10801 ttaaaaatgc tatagataca ggaataacta aagatataat tgcagaagga ttagatgttt
    10861 gtcaaactat tttaacaatg tttcaagttt tagaaataga aaactatatt actgaaggag
    10921 tagagacaca taataagaaa ctaaatggaa gaggatggga tctcaaagaa ataggagacg
    10981 atatacttaa tttaattaaa aattcaatga ttaatgaatt gattaatatg aacaagaaat
    11041 taaaatgcct tgatgaaaaa gaatttaatg caaaaataca atttttagat gtagataaac
    11101 tatggaattt gtgcatgcct ttagttaact taagtactat aaaaggagtt aatgaaagca
    11161 ttgatgaaat gaaaatggga gagtttctaa aaaatattaa ttaaagtaag gagataatat
    11221 aatatgttga tagaagtaac tgaaaataat agagaattaa aagtaggaga tattgttgaa
    11281 attaatggtg gtaataagtt atatatgatt ctttctttaa aggaaacaat aggtgggtat
    11341 gttattataa atatgcagaa tggatatggt tcatttggtg cttataaaag tttaagtcaa
    11401 ctagaattag acttgaaggt aagaggttat aaattatata gttctgatga ttacaagttg
    11461 caattagtgc ctagagaata agatatattt caaaaaagaa aaaaaggagc attacttcac
    11521 actcctgctt gtcaaaattc taaaaccgtt atcacaacat cattataaca taattaggag
    11581 tgtgaggtaa tgcaatctag caaaaaagac aatttgttta gtagtgcaga aggtaagtta
    11641 tataattata aaaaaataaa agcggaaata gaaaaaatag acattgatat gcagatgata
    11701 aaaaacgact acagaggatg tagtggagtt gaaattaaag aaaagacaag caagacatat
    11761 gatattaaaa gtgtaattga aatagagact gaagaaaaag aaaagaaaat tgccttaaaa
    11821 gaaaaagaga agagacataa agagttgata atagaaaaaa tagataatgc aatgaaaata
    11881 ttaagtgaag aagaaaaaaa gatagtacag tataaatact tttcaagtag caggacatct
    11941 tgggaatatg ttggcagaat gattggtttt tcagcgagta agtgtaagca aatgaggttt
    12001 gatattatag ataagataaa aggattgcta taaaaatgtc cgatttgtga cgattttaaa
    12061 ccgatttatg tctgttttgt gtctaaaaaa tgtccgattt gtgaccaatt tatgtctgtt
    12121 ttgtgtcttt taatcatgat aagattgtat tgtgagaaaa atatatttaa taaaaatggc
    12181 ttggaattta cgttctaagt cttttttatt gtctagagag gtgttgaaga ttaaacgtga
    12241 tataatagaa gaaatttcaa gtgctgcata tgtaccagac aatctaaagt attacgacaa
    12301 tgtaatgaga gaaactcctg gtatgtatgg aagagaatgt tctttagatt ttataaagaa
    12361 aaagcaagaa aaactcttga aactaaaaaa gaaggttaag aaaaattatg attctaatat
    12421 taataagata gactcatatc taaaaatttt agatgaaagt ataattgatg agtctgatca
    12481 cgttgagttg gttgtattta aattatattt gcaattagaa aatgttgtaa aagtaactga
    12541 aactgtaaat gacttaggat ttagaataaa gaccaatact tatgcaagag aaagaaaata
    12601 tagtacaaat gatataacct ctataatcac agatcctttt gctaatgtag ttgaagactt
    12661 aaaaatattg gtgcaagata ggcaaagaaa aaattatcat ggtaataagg agtgttttta
    12721 atgaatggaa gcacatagtg gacaaaaagt taatccaata aaagatatag aggatgtatt
    12781 tagactcttg aattttctag aggaatggaa cgaaagaaat tacttattag ccctctttgg
    12841 aatgtgtact ggacttagaa taggagatat attagcactt aaagttgcag atgtaacaga
    12901 tataaagtta gataaaaaag gaaaaaagat aagagtgtct aaggactgga ttagagttat
    12961 agaagaaaaa agagactatg atagagaagt tttcttatct gatgtaataa agagtgctat
    13021 agaaaactat actcaagata aaccaggaga agaattttta tttaaaagtg gcaagaggaa
    13081 gaaatttaat agacctatcc aaacaagaca agcaggaaga ataataaaag gagcagcaaa
    13141 aaaggtagga ataaaagaaa atgttgcaac acactcttta agaaaaacat ttgcaaggca
    13201 tatttatgat gaagaagaga ataagactta tgcattagaa cttataagga agattctagg
    13261 acataagaca atagaaatga ctagaagata tataggaata gataaggatg aagaagtaaa
    13321 agcaataact aaatttacta ataagttaaa gaaaagaaaa agaaattaaa atatcttgta
    13381 ttttatctaa aatttaatat gttacgtttt tcaacaacag gacattatta gaatgcaaag
    13441 agccttaaca atagagttta caaggaatag aatagattta ttaaaatgtc tgaaaatata
    13501 tattaaaggt catttattca agggatttat agggatgtag atatttaaat gtttatttag
    13561 aagctgagta aagaggtgat taattggatg tgaatgaagt tgaaataatc gacagtagct
    13621 atgaaaaagg taaagagttg acagaagatc agtgcaatat ggtaactcta ttgattacag
    13681 gtactacagt aacagaaact gccagaatta ttggagtaaa tagaaaaaca atctataatt
    13741 ggatgaataa agagcatgtt aggaaagaaa tggacagacg gaaacaggag ctaacaaacc
    13801 aagggaactt aatgatatta aaggatttag atagttatat caacaatatc aaggaattag
    13861 ctagtgacaa gagcgataag agggttatgt tggcagccaa tcagtatctt ataaatcgta
    13921 tatatggaac tcctacaagt acagttatac aagcagagga taatagtgtt ggcatgggta
    13981 tggatgcaac agagatagaa gcagctatag ctaagataag aataagaaca agcaagaagt
    14041 aattagatat gaaataaatc ctgcaagaat atgttttaat tatgtttgaa attaattaaa
    14101 gcaaaacata tattatatat atatttactt taataattaa atcatatatt gtatttaact
    14161 ttagttatag ctaatttaat aaaatttagt gagacttaat aattaaagtt tcatacagtg
    14221 aagtgatgtg gattattata ggatatgtga acctattgga aatgctaggt tctttttaac
    14281 tatgccgaaa atacatattt aaggaatagt tgaaagataa ttaataattc taatcaaaaa
    14341 aatatttctt gcaagaaaat aattcaaaat actttaatat atttattttt tttattaata
    14401 gggggtgtcc ttctattttg aaaaaattat tttttatgga aaatgagttc tagaaatttt
    14461 ctagcaaaat ttaaaaacca gagatgtatt tcaaagaggg tgattttatt agagaaaaat
    14521 ttacattgtt tattttatta attgcattac tcactgagcc taaaaaaata gaagaagtaa
    14581 gaggaagtaa atacgagtgg ataatctaga aagaaatata caattaatat ttagttatct
    14641 agcaaagact tttataagaa atggagcatc tataaaagat gctgaaaaag aagcaaatga
    14701 gattataaaa gccaattcaa acaatctttg gggagttaat ggtctcgctt atcaacttgg
    14761 aaaaataaac ctagaatttt tctgtatgta ttttatgcaa gacacatatt taccaaaaga
    14821 agataatgct gctgctccta tagctaaagt tcatcatgag ctatgggaag atatacaaga
    14881 atctataatt ggagatggtt cacagcaatt aggaagaatt tatccaagag ggacaggtaa
    14941 aagtgcattt ggagatttag cgacaactgt atggtcgcat tgctataaac ataagacata
    15001 tactttgatt tgctctgaca taggatctac agcagagaaa tttgtaaaag atataaaaaa
    15061 tgcattactt gaaaatgagt atattaaaaa agcatttggt gtccttttaa atgataatga
    15121 tagaaaatac atttgtaata gtactcaact agagctaaca aataaaactt ttattgaagc
    15181 tatatcatct tcatcaccca tgagaggaag aaaatacaat aataaccgtc cagatcttat
    15241 catacttgat gattatcagt cagaagaaaa tgttagaaca gaagatgcta gagagaaaaa
    15301 atttaaaaga ttttctgatg atgtaaaata tgctgctcaa aaaccagtca taagaaatgg
    15361 taaaactata aaaagaggaa caacttttat agcattagga accttacaac acaaagaatg
    15421 tttttatagt agactaaaaa atttaccaac ttggaagttt aaatgcgaaa aaggagtttt
    15481 agttgataat gtagatgaat tatttaattc tggtttatgg cttaagttca aagagatctt
    15541 atttgatttt aaaaatacaa atcatcttga agatgctaaa gaattttact gggaaaatga
    15601 aaaagaaatg aaattcccta tattatggcc aagtttttgg gattgtttag atatggctct
    15661 aagttattat gaaaatccaa catcttttaa gcaggaagtg caaaatgatg tcaattctat
    15721 aggagaaaaa tggtttaaaa ctgttagaac agaaactaga gaagaaattg aatcgcatac
    15781 ttttaaaaag acaatgctgt tatgtgatcc agcatctgcg ggtgggtcga aacatgacta
    15841 tagcgctttt cttgttggaa gtgaatcaga aaatggttta ctatatggaa gattggcaga
    15901 actagctaaa ataaatgcta gaactgattt tgataaatat atagaccata tgatttattt
    15961 attaaaagtg tattcagata taacacatat ttatatagaa aaaaatacat ttaatggcgc
    16021 agatgccaac caattagagt ttaaaataaa aaatgataat gttcttagtt atagagatat
    16081 agaaatcatt aatgagcaac aaaagaaaaa taaagatgat aaaatctcta ctttaatacc
    16141 tgttttaagc aaaggccaaa tgatttttgc agaagaagat aaggaattta tacagcaaat
    16201 acttgatttt acaggacaaa aatactctct acatgatgat gctccggata tatcatcgga
    16261 atttattaat cgaattaaca acataaaagt ttctgagaca gtaaatatct ttgatagaaa
    16321 attattaggt ttttagttgg gaggtaattt attggctaat agatcaaagt ttgaagaagt
    16381 tcaaaaaact ttaactaaaa aaggaaagaa gttcaatgtt ggaataggaa aagatgaaaa
    16441 aggatatttt gcatatactc atagagcaag gagtaaaagt tatatttcaa agcaagatat
    16501 tccaataaaa gttttaaaat ttatagagtc tacaggatag gatgtgttgt taatggaatt
    16561 agatttagac ttaataaaag acatatatga cgattggaac tctaataaat ccgaatatga
    16621 tactatgtat aagtattata aaggtgaaac ggatgctatt agcaactata aaatggttac
    16681 caagagatct aataataaga taaacactaa tttcttaaaa aagtttatta atgaagaagt
    16741 cgcttattct cttgcaaata agattaccta tacaagtaga tctggtgatg agaagattat
    16801 aaatgattta gaatactata cttgccattg gagtaaaaaa catgactcag atttattgag
    16861 gtatgcacta ttatttgggc tttgctatga actttactat gtaaaagatg atgaaataca
    16921 agtaaagata atcaagccaa cagatggctg tcattatgaa aatgaaaatg gagaaatagt
    16981 atgctttatt agagagttta ccaaaggttt cgaagatgac acgtatgtag atgtatatga
    17041 caaagaatat atatatcatt ttgattcaaa tttcaaagaa gttgaaagtc ctacagtaaa
    17101 caatatattt gatggaaatg ttccaatatc aatttgcaaa agaagtgaag aactaggaaa
    17161 aaatacaata tttaatgata taaaagggtt acaagatgct tatgaaacta atttaagtga
    17221 tataagtaat gaaattagtg attttagaaa tgcttattta accttttctg gatgtaatat
    17281 taaagaagat gatttgccaa ggatgaaaga acttggaata cttcaagtta atggagatgg
    17341 aaaaatagag tggcttatta aagatatgaa tgatacattt gttcaaaata cactatcttc
    17401 aataaaagaa aatatgtatg aaattacatc tcatataaat cacaatgaaa aaatgcaaag
    17461 taatacatca agtctagcag ttatagcaag attaatcagt acagaatgga tttgtagcca
    17521 aaataatgat agtattgcag atactttatt taataggtac aaattattgt gcatttggct
    17581 taataaaaaa tatggttttg actatgatta taaagatatt aaggctaaat ttacccctaa
    17641 gataccacgt gatgatttag ttgtagcaaa tatattaagc caacttggag ataaattatc
    17701 tactgaaact ggcttatctc aactaagttt cattgataat cctcatgctg aaatagaaaa
    17761 agctaaaaaa gaacaggaaa tagtttcaga gggagagatt ttattagatg aatcaaaaga
    17821 ttataactaa attgactgaa gatatatata aatctgctga aaataggaca aaaccatttt
    17881 ataagtatca aagagaaaat agggataaac ttttattaga aatagcaaag atattgctaa
    17941 cacacgatat tattaatgaa tacttaaata ttaataataa agataagaaa aatctaaggt
    18001 caaaacttaa taaaattata aatgagtatt ctaatagtta tgatgaagaa gtcaaggata
    18061 tagaaactat tttatctgat tcatctaaga ataaatattc aaatttgata attttattag
    18121 gagtaatact gaagataaaa gatgttagta atataaacaa taaagttata aataagatag
    18181 tgaataataa ggttgatgga aaacattgga gcgatagact ctggaagaat aaaaaaggta
    18241 tagaagagtc cttaaaaaaa gaaataaaga gttttctaaa tggaaaaact tctgtaaata
    18301 agatagaaaa agcaataaga gataaatatt caacaggtgc aggacaaacc agcagactaa
    18361 tagaaaatga agttgccaga tgtcaaagtg aagtaaatga gtattttttc aaggttcaag
    18421 ggattataaa agtaatgtat tgtgcagatt tagattctcg cacatgtagt gattgttcta
    18481 tgcataatgg agtgatattt tatgtcaatg aagataggcc ctctttgcca agacactgtc
    18541 actgtagatg tcaatatata cctgtttaac atgtattaat taatttaata ggtgttttta
    18601 ttatgtctaa aaaatatgag gtgaaatatg aaagttgaaa tattaggaac agaatatcta
    18661 gtgatagaga aacttgaaag cgaagatgtg ctattaaagg aaagagctgg atattgtgat
    18721 cactctgtta aggaaattgt tatagagaaa ataaattctg aagaaggttc attgaaagat
    18781 ttaagtgtat ataaaaatga agttgctagg catgaaataa ttcatgcatt tcttagtgaa
    18841 agtggattga agagttgtag ttcttgggct acaaacgaag aaatgattga cttctttgca
    18901 attcaatttc ctaaaatagt aaaggttatg gataaaatag aatgtttgaa ttaaattttc
    18961 aaaaaggaga actttataca tgttaaaaaa agaagtgttg gaattactaa aagaaataga
    19021 agatgatgca gaaatagatt cattgctgca atcaacagat ttattcaaat ctgcatctga
    19081 taaaaagctt acaatagaag agtttagaga attaattaat aatgatgcaa actttaaagc
    19141 tattatagat aatgaaaaaa ataaatatca cagcgaagca cttgaaaact ttaagaaaaa
    19201 agatatgcag acacttataa gtgctgaagt tctaaaaaga actggagcaa atgaaacaga
    19261 agagcaaaaa gcaataaggg aattaagaga aagcctagac aaactaaaaa aagaaaagca
    19321 gtacgcagaa aaagtttcta aatataaaga tattttagtt gaaaagaaaa tacctacaaa
    19381 cttaattgaa tatctattat ctgatgatga tgataaaaca aatgcaaata tagagatatt
    19441 tgaaaattca atgaagcaat atgtgcagtc aaaggtagat gaaagaatta aagatggttc
    19501 atacgttcca cctggcaaag atagtgcagg ttcgctatca gaaattagaa aacaaataaa
    19561 gcaagggcta aatagtcttt agtataaaaa aagaaaggat gattttataa atggctaatg
    19621 tattacaata tgtaacactt tttcaacaag aactagatat gcaggtaaca gttggctcaa
    19681 ctactggatg gatggagaaa aatgctggtc aagttatata taatggagga agagaggtaa
    19741 aagtaccaaa agtaacaatg gatggcttag gtgattatga cagatctaaa ggatatacgc
    19801 aaggcgcagt tacacttgag tacgaaacaa tgaaaatgga gatggataga ggtaggactt
    19861 ttatgttaga ttcaatggat gtaaatgaaa gtaacttcgt tataaatgca tcttctgtaa
    19921 tgggattatt tcaaaagcaa catgttatac cagaaataga tgcatataga tactcaaaga
    19981 tattttctga aacagctaaa gtagatgagc gtgtaaaata ttcatacgaa gctacagtaa
    20041 ctacaatatt aagtgaattg aaaaatcaac ttgcatcaat acaagattta tctggagata
    20101 tacctcttat tattagtatg tctagcataa ttgcaagcat attagaacaa tcatctcaac
    20161 ttgatgatat tgaatttggc gatggagata taaaaagaat tgtaaagtct atagacaatg
    20221 tacctataat aaaagttcca tcaaaaagaa tgaaaacaga atacgtattt agagatggta
    20281 aaacatctgg acaaacagag ggtgggttta ctgaaggagc aaaggcttta gatataaact
    20341 ggttaataac tccagcatct gctccaattg cagtatctaa gactgataag ccaagaatat
    20401 ttactccaga tcaaaaccaa acagcagatg cttatcaaat agactataga aaatatcacg
    20461 atttgtggat attggatgaa tatttaaaac tttgtagagc atctgtgaga caagctaaag
    20521 ctgcacaaga ataaggagtt gttttaaatg tttgcattga taaaaaataa cataattaaa
    20581 gttgttgaag atgaaaattc gagagataaa ttgatttcaa aaggatttaa aatatattca
    20641 gaagaagtga ctaaagagaa tattgagaaa aaagaagaaa aagttaaatc aacctctaat
    20701 gatttattta atctatctaa accagagctt atggaaattg ctaaaggcaa gaatataaag
    20761 ggatattcaa ctaaaactaa agaggaatta attcaactac tagatgagta caatgcttga
    20821 taatgttaaa ttagttttag gaatagaaaa tgatagatat gacaaattaa taatccttta
    20881 tataaataaa ataagtgaga tggttttaga atattgttct aggcaggaac taagtatagc
    20941 actagaaggg tttgtagaag aaaaagtaat atcaatatta tcttcaagat ttgaagaact
    21001 taagaaaaat agtggacttg ataatctaga aaaaagctta aaagctataa ctagaggtga
    21061 cactaagata gaatataatg ctttaacatt acaaactgct gaaaatagca tcagttcatc
    21121 atctttttta actgatactg ataaaaaatt tctaagccca ttttgtattg ttagtatgta
    21181 ctaaggggtg attaaatgac tgaagcagat atattagaaa tgacatacct tgataaaatg
    21241 actataatta gaaaattaaa aggctggaat gaagaaacat gttctaatga ttttagtaat
    21301 gttgttatag ctgaaaatat cccatgtgca atttctagaa aagaagaagc cattgtaaat
    21361 ggtgatattg gatctatagt tgtaaataga aagttatttt gtagaccaga agtagatata
    21421 aaaataggtg atactataaa tacaacctat aatcatggag aagaagctgt atttaaggtt
    21481 tctaaggcaa atatatatcc aagccatatg gaaataccta ttacagaatt ggagaggata
    21541 taatgggaat agataattta ggagtctttg aagcattagc cttagattta ggtctaaatg
    21601 catcaaactt taatagaaaa atcaagaagt ttacaaaaga aactgggcaa gaactagcag
    21661 aaaatataag agatgaaact cctgtaaaaa ctgaaaagtt aaaaaatgga tgggaagaaa
    21721 agcaaccaga atatgatact tatattgtta aaaatgaagt tgattatgct actttagtaa
    21781 ataatggcca ttcaactaaa aatggtggca ttgtccctgg ttcttttatg gttgaaaagg
    21841 gaattgaaag aacaaaagaa aatatgacaa gtgcaaaagc aaatttattt gattggtagg
    21901 tgattagttg cttacaaaca gagacattat aatagcagtt aataaaagaa tcgaaaatga
    21961 gtttagatat atagatattt ttattaatga ggataatata caaggctttg aggaggcttg
    22021 tttttttgtg caacttttac ctgtttctaa taatattgca aataaagttt tagatactaa
    22081 aaagatattc atagatattg aatattacca aaaggctaga aaaaataaat taaatctata
    22141 cgacatacaa agtagattag aaaaaatatt taacagaaac attagagtta aagatagaag
    22201 tttaatcatt gagaatatat ctccatcaat cgcaaaagat cctgtaggat ataaactaac
    22261 ttttctaatg actgtatctt attttgaaga agtctatttt gaaaatgaaa catttgaaat
    22321 tatggaaagt gtagatttag agataggaag tgattaaatg ggtttaccag aaattaatat
    22381 ttcttttatc caagcaggaa aaacttttgt taaacgctct ggtggtattg tagcgcttat
    22441 tttaaaagat acaaaaaata tagggttagt tgagataaaa gaaatagagg atattccaga
    22501 aaattattct gctgctaatt tagattatat aaaaatggct atgaagggaa atgtatatcc
    22561 tccaaataaa gtattagttt atacattaga tacagatgaa tcaatagatg atgcactaga
    22621 ttttcttgaa acttgtgagt ttaattatct ttgtatgcta gatgagacag aacaagattt
    22681 gcctaaaatc aaaacgtgga ttaagaaaat gagagaagat aataaaataa aggttaaagc
    22741 tattattgca agtaattcag cggattatga aggtataata aactttacag caactgatat
    22801 tgaagttgaa ggtaaaaaat acacttcaaa tgaatttctg ccaagaatag caggttttat
    22861 tgcaggaact ccatcaactc aatctgtaac atatgcagaa ataccagaag ttactaatat
    22921 acctaagcta acaagagctg aagcaaatac aaggataaat aatggagagc taattttaat
    22981 aaaagaaagt ggagctatag taatcgctag aggagttaca tcctttacaa caattactga
    23041 ttcaaaaggt gatatattca aaaagattaa gttggttgat acactagatc aaattcataa
    23101 cgatattaaa aaaataattg taaaaaatta tataggaaaa acccaaaaca cttatgataa
    23161 taagtgttta cttattgttg caatacaatt atatcttcaa gaattagaaa aagatggttt
    23221 aattgatgaa ggttcaactg tagaaattaa tttagatgct caaagagcat ggctaaaaaa
    23281 acaaaatata gatatatcta atatggaaga acaacagata aaagaataca atactgacac
    23341 tcttgtattt ttaaaagcaa gaattaaatt aatagatgca atggaagatg tatatttaga
    23401 catctctatg tagtttggag gttaaaattt gaaagctaaa atagatgcaa aaagaataat
    23461 atctggtact tttggagagt gttggctaga taataagcaa cttacatcaa caaaagggat
    23521 ggaagccaag gttgatatag ataaagaaga aataaaatta tgtggtcaaa taatgaccac
    23581 atataaaatg attggtgcaa ctggaaaagg atctataact ctttataaga taagtacctt
    23641 atttggcgag tatgtcgaaa aaatgttaaa tgaaggtaaa gaatttaaag taactataat
    23701 aagtaaattg caagacccag attcatatgg ttctgagaga attgcacttt atggatgtat
    23761 gtttgacgac ttaactttgg ctgattggga agctgcgaaa gttggagaaa tagaagttcc
    23821 attcacattt gaaggctttg aatacttaga taaaataaaa gaaaaatagg aggattttat
    23881 tatatgaaca cagtagaaaa attattaaat atggatgcag gtaaattaaa aatgccatct
    23941 agcgcatttg aattatattg taaaaaacta gatgatactt tagaaataga gtgcaaggct
    24001 attgatccag aacgttttga cgaaataaga atgaactcta tggacgttaa tagtggagat
    24061 gtagaaaaca ttaacatata tgaacttaaa gtaaatacaa tattagaatc atgttctata
    24121 tttagcaata tggaattagt taaaaaattt agtgcaatta cacctaaaga attaattaaa
    24181 aagctattac ttagtggtga aatagataaa ttatacgagg aggttaataa agtaaatgga
    24241 atagatgcag agggcgataa aaaaagacaa aaagaaagag ctaaagatat aaaaaactaa
    24301 ttaaaacgga tggagaagtt aacttgatgt acttattatt taagtataaa ggtataatgc
    24361 catccgtttc ttatcgtata ctaagaggcg aaaaagaaat tgttagtgtt tttatggatc
    24421 aagaattaga tgaaagagaa gaagaaataa aatctggtgc tgggaggttg ttttagtata
    24481 tgagttctaa tacaaaagtc ctacaagcaa taataaaaat gagagatgaa gcaagttcaa
    24541 cattaaaaag agtagcagca aatactagag ctctacatga ttcatcttct caagcatcaa
    24601 gaacattaaa taatgttcaa agtaatttaa aaagcgttgg aactagtgct ttaaaagcag
    24661 gaggtcttat cactgctgga gttggtggcg catttgcagc tgcaactaaa gttggaacag
    24721 aatttgaaag cgctatgtcg caagttaagg ctacatttgg aaattcctta aaaccaggag
    24781 attttgaagc tctgtctaag gctgctagag atgccggaaa agctacaact aaaactgctg
    24841 ctgattctgc taatgcactt caatacatgg ggcttgctgg atgggatgtt caaaagtcaa
    24901 tgcatgcctt aatgccagtg cttaatttat cagaagcagc ctctatggat cttggtctag
    24961 catcagattt agtaactgat agcatgagtt ctatgtgctt agaagttgat aaggtcagta
    25021 aaagtggaaa agtattagcc ggtgcaggtt taagtgagta tttagataaa gttgcaaatg
    25081 catctactaa atcaaataca agtatacaac agttgatgga agcgtttaat gttgcaggtg
    25141 gtacatttag caatttaaaa gttccgcttt cagaagcaac tgctatcatg ggaatacttg
    25201 caaacagagg agttaagggt acagatgcag gccatgcttt aaactctatc atggtaaatc
    25261 ttacaacagg tgcaggccaa gcaggtaagg caatgaaaaa gctaggcgta agtgcatttg
    25321 ataatcaagg taaatttaaa ggtatgtcaa atgtacttaa agaagttgca aataaaacaa
    25381 aagatatgac tgataaacag aaaaattatt acttagcagc tataggagga aaaactcaat
    25441 taaaaacgtt acaaaagtta cttgatggtg tgggaaatga atatgatagt ttaaagaaaa
    25501 aaatagataa ttcaaaaggt gctttaaaaa caatgtctga tgtaatgaag gataatacta
    25561 agggtggact tgcaacctta aaatcagccc ttgaagaaac tggaattact atttactatc
    25621 aactaaagcc agcaatagca tctgtagttg ataatttaac taaaatgact gtttggttta
    25681 ataatctaag tgaaccaata aaaagagtca taactaatgt aatgctttca gttggagcat
    25741 ttggagtact tttattaact ataggggctg tgtctttaat tgtttcaaaa gctataggta
    25801 tatttggctt ttttgttcca ataatattaa gagcaaaagt tgctttcaga gcttttgtaa
    25861 ttggtatgaa accattaagt gcagtatttt tagctgtaat gggtcctata ggatggacta
    25921 taacagctgt aattgcattg tcagtagcat ttaccattgc ttatgctaaa tctgaagtat
    25981 ttagaaataa ggttaattca ttagtaaaat catttacaga atttgcaaaa cctatagtaa
    26041 atttttgtat gcctgcactt aaacaattag gggaaaaatt taaagagttg ttatcagcat
    26101 ttggtccact atttgcgtct attttaaact taggaagtgt aataatgaag gtactaggac
    26161 ctgttttaat tggcgtaata ggttttcata tagcaaaatt agttttgatt ttatctactc
    26221 tggcaactgc ctttaaagct gtagttgata ctataactaa tgtattaaat ggtctagcat
    26281 ctattttaaa tggagtattt aatttaatta taggtattgt cacaggcgat agtaaaaaga
    26341 tgatgaatgg agttaaacaa atatttgatg gtggagtaaa aattataaaa tcaatttgga
    26401 aaggtttcat agattttgta actgctccta tacaagctat agctgatata gctgacagct
    26461 tatttaaaga taaagctaaa tggcttacag ataagtggaa agatttaaga acattcttag
    26521 aaaatccaat tgaagcaact gccaaaatat ctactaaaac aaatgtaaac attccaggaa
    26581 gtaaagcagc tgatgcagct gggaaatcta caccaaatac aggtggatta aaacaaacaa
    26641 tagcaaatct aaaaggttca tttagtgatt ttaaaacttt tattaaacct atgacagatt
    26701 ctataagtca aggagttgaa tctggaaaaa ctaaatttac agaactgcaa aacaaaatat
    26761 taaatgatgt taaacctgca ttaaaggtcc tagaggattc tggagtaaat tttaaggata
    26821 agtttaagga gtcaataact tcaataaagg ataaatttaa tgaattaaag aacatattag
    26881 ctggaaatat taaaacaaat atagataatt tagtaaaagc atttgaacct ttaaaacctc
    26941 atttagataa tttgaaagag tgttttgata aggttaaaaa agcgattact gattttttta
    27001 ctccagccaa acaggttgga gatacagtaa acaaagtaag ccaaccaatg gatagtgtta
    27061 aaaagtctac tgatggaatt aaactttctt tccaaagttt aactcaagca cttgctcctg
    27121 ttaaagcttg gtttcaagga tttatgcctt tcctatcaca aataggacaa caacttttac
    27181 catctatagg aattgcaata gctggtgttg ttggcggaat aattttagca tttacatggg
    27241 ttttaaatac attaaccgca ataataacaa cagtatctgg aatcataaat ggaattatgt
    27301 cttttatatc tggaattatt aatgttatta tggggcttat aacaggaaat gctgatcaaa
    27361 ctatggatgg acttaaacaa atatttgata gtggagtagg tgtaataaaa tcaatttgga
    27421 atggccttgt tgatctagtt agtggtgtac tggaacctgt aatagatctt gctgactcat
    27481 tttttaaaga taaagttaaa ggggtaaaag aagcttggaa taaacttaaa gatttcttta
    27541 aacatccaat taagggaaca atagaaatat ttaaaaaagg tcctagttta caaattggaa
    27601 aaggtgcaga tgctgctggc aaaagtccaa gaaaagcatt tggtataaat agagttccta
    27661 gaaatgatta tttaattagg gcacatgaag gagagaaact tttaaccaaa caagaagcca
    27721 atcaatacaa aagaaatagt tcaaatggaa gtttatttct tgataaattg gctgatacaa
    27781 taattgtaag agaagaggct gacatagata aaattgtaag aaaaataaac aaaaaaatat
    27841 ctcttgcaaa agttggaggc gttttgtaaa ttatcgtgct ataattgtag tatataattt
    27901 acagggggtt atgttatgaa taaaaaaata ccagcattag caatattaat tgtattatct
    27961 ttaagtttag ttggatgcag cacaaatgat tcaaaaaatg gagatagtaa acctaagcaa
    28021 aatacagagg aaattaaaaa tcataaaata ggcgaagttg ttgatttgga cggagcaaag
    28081 gtatctgtta aaaatgttag aaaactagaa tcagatagta atgtatatgc agttgagatt
    28141 gaaatcacca atacaggaga tacagatctt gaaagtgatt tccttcaaaa ctacatacta
    28201 aaaacggatg ataatacagt tggaaatgca ataaataatc aagaattaaa tggagaatta
    28261 ctatttgaca caatacatcc aggagataca ttaaaaggtg agatagcatt tgaacttaca
    28321 aaagatacaa agccaaaaac aattgaaatt gcttatgaaa atgtaggaaa atatacagta
    28381 tttgatttat aataattatc ttaaaagaca cttattgaaa taagtgtctt ttattttagg
    28441 aggagtttaa tggaaatatg gttaagacaa gcaaataata catttaggtt tcctgttatt
    28501 ccaccctctt ttgagataaa tggaagtgca acaataaata catctaatat tttaagtgtt
    28561 ggagatattg cagtatttgg agggttaggg cttaaaacta tagaactatc ctcttttttc
    28621 cctaatcaag aatatagttt ctgtaattat aatggatttc caaaaccata tgattgtgta
    28681 aatttaatag aaagttggat gaaagaagga tatatattaa gatttatcat tacagaaact
    28741 aatataaact ttgaatgtat aattgcagat tttaattata gagaacaaga ttattcaaga
    28801 gatgtttatt ttaccttaag tctaaaagaa tataggagga tacaaatatc caaagtaaac
    28861 attaataatg atgaaaaatt atcttctgaa aaggatgttc cactaacaaa aggatttgat
    28921 actaagcaaa aaacacataa ggttgttgaa ggagataccc tttttaagat agcaaaaaaa
    28981 tattatggca atggagattt gtgggagaag atttataaag caaatgaaga taagattaaa
    29041 gatccatctg taataaaaaa tggttggata ttaataattc cttaaaatgg tggtgattta
    29101 taattaataa aatcaaaata caagttcata taaaaaacgg aaatatatat aatgtaactg
    29161 atttagtgga taagtgtact tggtctggtg attatagatc accatcaaga actttagaat
    29221 tttctataat tcaatctgca agtgatatta attttaggca gatagatatt ccagtagcaa
    29281 gtacagtttg tttctatgtt gatgataaag aactttttag aggaatgata atagatagat
    29341 ctaaagactc tagcaataat aatatagatt ttacagctaa agatatgggc tttttacttt
    29401 tacaaagcga agtgtcatac aattttaaag ataaattagt tgaagatatt gcaaagcagg
    29461 tttttgtaga taataaattc ccactaggta atttacctaa aactaacgtt aaatatacaa
    29521 aaatgtttat tggtgtaact ggctatgata ctataatgag cgtttataca gaagcaagca
    29581 agacaactaa aaaaaagtat atgatagagt ctaatcttga taaatttaat gttattgaga
    29641 agggtgtagt tactttaaat ataacatttg aagaaggctt taatcttatc aatacaaatt
    29701 tttctgaaag catggagaat gtaaaaaata aagtactagt tgttgatcag tatggtaata
    29761 aaattagtga gaaaataaat gactctatat ttaaagacgt tggggtaatt atgcaaaagg
    29821 taatacaaca acaagaaaac agtactatag acatagatgg agagtttaaa ggaatcgaaa
    29881 aaacttgctc tttaaaagga tatggtgata taacttgtgt aactggtaga ggtgtaaaag
    29941 taaaagactc atatacaaaa ttaataggtc ttttttatat agatacagat aagcatacat
    30001 ggcaaaatgg agattatcaa attgaactag agcttaattt tgagaatata atggatgaaa
    30061 aatctgctgg tcaagatgaa caaaaagaag atggttcaga gtatactgga ggaacagaat
    30121 actctgctga atttactgct tattgtccta gaaaatctga gggtggagat aaagattgta
    30181 gaggtaaaaa actagatcct tctaaaaaaa catgtgctgc tcctatggtt ggaacatatg
    30241 aaaaatctta ctatacaaaa gatttcttaa acaaacatcc tcttatgaga tatggagatg
    30301 aaatacaatt ggttacaggt gtttcaagtc gagatggaac gtataaggta aatgataatg
    30361 ggcctgcaat aattatagaa aaggatggaa catatcatat agatatttta tttggaaatg
    30421 ttgaagaagc caataacttt ggaagaagaa aagggaagat tataattggt ggttattctg
    30481 gaaacgtgac agaaaaagca aaaacagtaa tttcagaagc aagaaaacat cttggaaagc
    30541 catacaaatg gggtgggaat ggaccaagta gctttgattg ttctggacta atggtatatt
    30601 gctttaagaa ggttaatgtt aatttaccaa gaacctcaag tcagcaatct aaggtaggta
    30661 agaaagtaga gaaaaataat ttacaggcag gagatttggt atttttccat aatccagtca
    30721 gccatgttgg actatatgta ggtaatggag aatttttaca tgcgccacaa acaggagatg
    30781 tagtcaaaat aagtaaatta agtaatagaa aagattttaa tacagctaga agagttttgt
    30841 aaaagaggtg atatcgtggc agatccaatc aatgaattta taggaataat gagacaagaa
    30901 ggtaaatttc ataatgaacc ttcttttttt attggaaaaa ttaaaagtaa attaccagat
    30961 ttaaaaatag agataaataa catcatatta gaaaaagaag atatcttgat agacagttgg
    31021 atgcttgata gacagataga gttatttgat acagaaacaa gtcaagaaca taaacatgaa
    31081 ataaaaaatc cctttataga tacttttgaa tctggagaca cagtaataat gtttaaaata
    31141 ggtgaaaaat ttgctgttgt aagtaaattg gtgagtctag atgaataata ctatattccc
    31201 tttcatgggt gttccagaag attatatttc tccaaccaat gaagaattac ctatttttaa
    31261 agaatttgct tgggatttta ataaagatga aaagataatt gaaaatggag actttaaaat
    31321 agttgaaaga aatgaagcaa ttaaagtatg gatttacaaa acaataaaaa cagataagta
    31381 tgttcattta atatatgatt gggattatgg aacagatata aaaaatctaa tagggcaaaa
    31441 gtatactaaa ggtcttactg aaagtgaggc aaaaaggtat atacaagaaa ccctattaac
    31501 taatccatat atattagaag tcaatgtaac aaatacaaaa tttaaagatg ataatttatc
    31561 tataagttta aatgtaaaaa caatttatgg agaggaggag attacttttg cataatggtc
    31621 aatcatttag tgtcttaaat aatagaactt taaataacat aaatctacct ctttataaag
    31681 gtcaaggatc ttctctttat aatatagtct ctccaataaa ttcagaactt gcacagttat
    31741 atatagaact ttcttatata cataagagag tttttattca agataatttt gatgattttc
    31801 ttgatagaag agttaatgaa tttggagtat acagaaagtt aggcacagag gcaataggtg
    31861 aggtaatatt tgagggaaaa ataggaacta aaataccaaa tggaacaata atatcttata
    31921 gtgatttatt atttgtagtt attaaagata ttacgataga tgaagatagt aagttaaatg
    31981 taagtcctat acaggcttta gaggttggta ttaagtataa tttatcagct aagacagaat
    32041 ttaaacttat agaagaaata aatggagtaa ctaaaattta taatgaacta gacataaagg
    32101 gtggaacaga aatagaaact gatgaagaat taaaagagag gttctataaa atacaaaaaa
    32161 atcaagcaac aagcgggaat aaagcacatt atcagtcttg ggccttagag gttgaaggtg
    32221 tttataatgc aaaagtaatt cctcgctggg atggaccagg tacaattaaa atcctaattt
    32281 atggtcaaaa caatcaatca gttgacaatg aagtattaca aagatgcact gaacatatag
    32341 aagaggaaaa acctattggt cctactatta caattgttac accaagtact tttgatatta
    32401 ccattagtgc aactttaaca cttgaaaatg gatatgatat tgaatctata aaggttatgt
    32461 ttttggatat tataaattct tatttaatag aaaattcaaa agagattatt tatataaaaa
    32521 taatgagttt gttagcaagc atagaaggtg ttcacgatat aaaaaatctc cttgtaaatg
    32581 atgatgttaa aaacataata gttgatgaag aaaaagttcc tgctgtttca agtgctatat
    32641 ttgatatcga ggtgagttaa attgaagtta attgattacc tgccaagttt tgcaaataat
    32701 gaaattgaca tacaaatcca ggaagcactt gaaaatgaac ttctaacatt aattgacgaa
    32761 aaagatgatt tattagatca gttttttata gatacagcaa cttgggggct tgatgattgg
    32821 gaaagtctct tagatataaa aataaattat aagttggatt ttgatactag aagaagtaat
    32881 ataaaagcta aattaagagg caagggcaca actacaatag aagttataaa agctatatca
    32941 gaagcttata caaagactaa tgttgatgta aaagtgttca gtaatctatt tagttttaca
    33001 cttagttttt taacaaatga ttgtagttat aacactattt tagagttaga taagaaaata
    33061 gaggaaataa aaccttgtca tttagagcat aaatttgaaa tgattttatt caataaaaat
    33121 aacatgttta caggttctac aatgaataca ggtgaaacag ttactatata tccttacagt
    33181 acaactgata taacttcttt tggagaattg actataacta caggtaatga cagaagtatg
    33241 gaaaaagtaa cattatatcc tagacaggag gtggtatagg ttggcagaac aaaaatatta
    33301 tacacttttg acaacgatag gaaaagcatc tattgcaaat gcaacagctt taggcaataa
    33361 agtagattta gttaaattac agcttggcga cggagcaggg gcagaatata atccaacaga
    33421 agaacaaact tctctaaaaa acgtagtttg ggaaggttct ataaataatg taaagataga
    33481 cgaagaaaat ccaaactgga tagtaataga aactgtaatc ccgggtagtg tgggtggctt
    33541 tatgataaga gaagtaggga tatttgattc aaaagaacaa ctaattgcag tatctaaata
    33601 tccagaaacg tacaaaccaa cagctgacag cggaagtgta aaagacttag ttataaaaat
    33661 tattttagta gtctctaata cttcaagtgt aaatttgaaa gtagacccaa ctgttatttt
    33721 agcaacactt aaagacatac aagatttaga ctctaaaatt gatacaacta aaacagaatt
    33781 aacaagcaac atagaaactg ctaaaacaga gttgaataca aaaataggag atacaacaca
    33841 acttactaca acagataaaa caaatatagt taatgcatta aatgaggtaa aaactagtgt
    33901 agatagcata gaaacaacag cagagaaaac aagtataaaa gatatagata acttatttga
    33961 aagcgacaat gtagagggag cattaaatca attagcaata aattataata tattattgga
    34021 aaaacaggat aatttagaaa cagaagtgaa tggacagaga ataaaaggaa tttcaatagc
    34081 aaatagtttg atagacatga tataagagag gtgagaatat gacagaaaag ttgactgata
    34141 atgctagttt aagagaactt atgatagcat ttgaaagtgc aaaaaatgat ttgcagatag
    34201 ataaaaataa tatagccagt acattgggaa gtccattttt aggaacagat aaattagata
    34261 caactaaaac aaaaacacaa acattaaaaa atacacttgc aagtaatata actaataaag
    34321 gtattgtaac aaacagtaca gaaagtttgc aagatatgat aagtaaagtt agcagtataa
    34381 aagatttaag acaagcaagt ggtacagcta gtgcatttaa aggcagattt tatgatgatt
    34441 attactacct atggagtgta aatttaccac aattaaattt tgtgccactt gttgtttttg
    34501 tctatgctga ctatagacgg ctttcgattt gggttaaaag tcctttttct aaatatattt
    34561 tttatggtaa aaaagataat gtaaaaacag agttaaaaga tttttatata aaagagttag
    34621 acgatggaat ttctacagag acagttatac atctctatgg ggcttcagga gacacttatg
    34681 aagtacaatg gttagcaata ggtcaggagg cttaaatatg gaagtaggaa aattaataat
    34741 ttatgataat aaagggaaaa ttttatttca aggtggagaa ggaaaaggag atttaattcc
    34801 atatgacaaa gacatgaaag ttgattgggt agagataaaa tacaattcta tagattattc
    34861 taaatacagg attattggaa taaatgtaga aacaaaagaa cctgttttag aagaaataaa
    34921 agtagaatta acagaagaag aaaagagaat acaagaatta gaaaatcaaa tattgattgc
    34981 agaaaacgaa aaagtaggag gaattttata atgaatataa ataatgttgt cgtaagaata
    35041 ttggcagaga gaattttaag tggaggttta aaccctctaa aaaatcgaga atttgaactg
    35101 gatgatgtaa ctaatgcaga gtacagaaaa gcagtagagg attatataat tagagaaagt
    35161 ggagtaatag aaggaacaga actaacagta taggaatgtt ccttttttat ttcaattaat
    35221 taggaggctt acatgaatga agaacttttc gaagcagatt taaaaagaca tgaaacaaga
    35281 ataaataagc atggagaaga aatagacgaa ttaaaaatag caaatataga gtctaaagca
    35341 gagttgaaag cattgtgtga gaatctaaac tcacttacaa gtatgctcaa atggctaatt
    35401 ggtacaatga ttacaacatt agtaggattc tttatatttg cagttcaaag aggaatattt
    35461 taattaggag gataaaagat ggataattta ataagtttta taccagagca gttgctaatt
    35521 ttagtagctg ctctttatgt tataggagca ggttgcaaga agtataaaca actagataat
    35581 aagtatattc cagtagtatt actagcactt ggaataggtt tctcaatatg gatgttagga
    35641 ttaaatcctg ttgcagtctt acaaggcgtg atttgttggg gtatatcaat aggtataaat
    35701 caaacttaca aacaactaaa ggaggaaaat aaataatgaa aatagcaata gtaccagggc
    35761 atacattaag tggaaaagga acaggaacaa ccgggtatat agatgaagga aaagaaaaca
    35821 gaatactaac tgatttaata gtaaaatggt tgaaacaagg tggaactact gtgtatactg
    35881 gaaaagtaga taaatctaat aactatttag cagagcaatg tcaaatagcc aataaacaag
    35941 atatagactt agcagtacaa atccatttca acgcaaataa aacaacacta aacgcaatgg
    36001 gtacagagac aatatacaaa actaacaatg gtaaggtata tgctgaaaga gttaacaaaa
    36061 aactatcaac aatatttaaa aataggggtg caaaatcgga tgcaagaggt ctttactggc
    36121 ttaagcatac aaaagctcca gcgatattga ttgaggtgtg cttcgtagat agtaaagcag
    36181 atacagacta ttatatcaga cataaagaca tagttgctaa gttaatagca gagggtatat
    36241 taaataaaaa tataaataac aaagagaatg gtgagggaaa aatcatgtac aaacatacaa
    36301 tcgtttatga tggagaagtt gacaaaatac ctgcaactgt agttggctgg ggctataatg
    36361 atgggaaaat actgatatgt gatataaaag attatgtacc aggtcagacg caaaatcttt
    36421 atgtcattgg tggtgcagca tgtgagaaga ttggttctat gactaaagaa aaatttacta
    36481 tgataaaggg taatgataga tttgatacac tttacaaagc attagatttt atcaaataat
    36541 aacttgttgt aatatccaat caatataaaa tttattcaac ttaggaggac ttgcaacatg
    36601 cttaacatag aaaaatataa actatacaat ggaaactgtt tagaaataat ggatctaata
    36661 gaagataaat caatagattt aattctatgt gatcttcctt atggcacaac taattgtaaa
    36721 tgggacacaa taataccttt caaatcaatc tggaatcaat ataatagaat tataaaagat
    36781 aatggagcta tagtactatt ttcagcacaa ccatttacta ctagtttgat aaattcaaat
    36841 attaaaaact ataaatatag ttggtactgg attaaaaata aatctacagg attcgcattt
    36901 tctaaatacc agccacttcg aaaagtagaa gatattaatg tattctataa aaagctcctt
    36961 tatacaatcc tcaaaactta gaaaaattag ataaacctat tacatgtaaa aagaaaaata
    37021 aaaataaaga tggaatatat agacatcata ctttatcaaa agaatatgtt caagaatata
    37081 caaattatcc taataataca ttgtatttta ataaagaaac aaattgcatc catccagctc
    37141 aaaagccagt agatctttta gaatatctta taaaaactta tacaaacgaa aatgaacttg
    37201 ttttggataa ttgttttggt tctggcagtg taggaatagc ttgtgcaaat ataaatagaa
    37261 agtttgttgg aatagaatta gatagcgatt attttcttca aggtaaaaat agaattgaaa
    37321 gagcatacag aaacaatgca attgacaaaa acatagtaaa atcctaatat tagagaaagc
    37381 aaaggataat agataaaaaa actatcctcc tttgctttct ttatttttct atttctttta
    37441 ataatcttat acgttgcaat tacgcatctg ttaaatctaa gattaaataa ataaaaagac
    37501 tagaaaattt tattatcttc taattctttt tatttaactt atcacaataa aaataaatca
    37561 ttttttaatg acatttatat gatatttatc atatttagtt ttgaaacatt ctagcgttgc
    37621 tttttttctg gaataactta cctgtactat cttaaataaa aaaatgctac tatattataa
    37681 accaaatata aaataaatga tttagagcca gattatttag atagatattt tatacgatta
    37741 atgtccactg tgcataaatt taaaaatcta gatgaagaag attaaatatc tcccttcatc
    37801 tgctcttcac atattttaat agactttcaa gcaataattg acttagcaaa tcatgtttat
    37861 cataatgttt attctcatct gcaaatttat taaacatttc ccacactgta ttatttaccc
    37921 taattgtagt tctttttata gactgatctg gaagatctat agatatatca ttttctttaa
    37981 tgctatctat atgacattta tcatctttag ttttaaacca ttctatcaca ctttttatat
    38041 tatcaatttc attagataaa tatttgatat catcatcaat cttactgtta atgctatcta
    38101 tatgatattt atcatcataa cttttattat cttctaaaaa cctgtttcca actcttttat
    38161 atcctttctt attaagccac ttccttatag tactttcatt tgcccctaat tcatctccaa
    38221 cttttttaag actttcaaat tcacttaatt tattattaat acaatcaaca acttcattaa
    38281 tatttttttc tttcaaatca ctccaagtca tataaaacct cctcaaaaaa ataatagatt
    38341 acaaatatca tatatttgta atctattata catactataa actaaatcct tttttatttc
    38401 atcatttttt tgtatccttt tgcatttgca aaaacatgtt tatctacatc actcatgact
    38461 attatgccag gtatattctt tttaaatgct tcacctaaca atttagcacc tccacccata
    38521 acgacaacag cagaagttga tattgggtaa tttaatttca attcattata tatttgttta
    38581 aataatccaa cagacgcatc tatatttttc tttatatctt gtttttcacc atttaggtaa
    38641 aaccctttgt ctatgtagtc ttgtattttt tctcttttta tctcaacttt acccagaaat
    38701 ctttcatcca tttcaagaga tttttttatt gcatcatata tatttatcgt tccaacattt
    38761 acagaatatg gatcagtcgc tttattccct tgtacaagcg caatatctgt tgtctttcca
    38821 ccaatatcaa ctaaaacaac atctcttcct gcaatttggt caactatgtc ttttgcataa
    38881 tataagtatc ctgtaactcc ctctggaaac acttgaacag atcttattgt tatatctctt
    38941 ttagtagttt catttccttt tgcaaattca acatgatatt cttttccttg taatttttca
    39001 atcaaatctt ttcttaatcc tccaaattgc ttcactggta gacctaatgc tagatcaata
    39061 acttcatcat ctgtatttct gcatatagcc ccaagtaaaa gtggtaagaa gttttctttc
    39121 tcatatttaa ctatatttat ttcctcgtca ccttcgccta aataatatgt tttattacct
    39181 attttaatgc tatctgaatc tgaaccaaag ttttttacct ctgtaacttt actttcaaaa
    39241 atatcatctg tacttgtttt aactgcataa tttccaatat ctatacctaa tttactcatt
    39301 tatgttattt cctcctttta ttctaaactt ccaaaatcaa aattgttagc tggatcttct
    39361 atttcttttt tctgttcaac tacagcatta tccttttttt cattcatttt ttcccaaacg
    39421 agttctttta aataagctgt agctgataat ttctcatcta aaaacttttc aattgctttt
    39481 tctttatcat tgtttttaaa tgttagaact atcctactca ttcaaccact tccttttttg
    39541 taaaatatta taaaatttat taatatttac atatatttta ccaaatttca catagtaatt
    39601 caatagattc ataagatatt ttttataata ttttataatt ttttacgtaa ttttataatt
    39661 ttttataaaa tttatcattt tgtatataat taagaacaca ctttttataa ttttgcataa
    39721 aaaaaagagt gtttcatata tttctaaaca ctcttttcta ctttttataa ttatctaatt
    39781 gtaaaagaag cttcagctgg actagagaat aatataatac ctcctgtaca aacacctttt
    39841 cctttaaatt tagcaatata tgaacccttt tttagccctt tgaaacttac tacaccagtt
    39901 ttagtagttc cagctaatct aaaacttaca aattgagatg atacaatttt gccagcctta
    39961 tatacatcta tatttccttc aaatcctaaa atatttccaa gcgtagatgt tactctccaa
    40021 tgtaattgtc ctccactcat tgctgttaaa ttagcagtac cacctggaac aacaacagat
    40081 ttagtcgaag gcgaatctga gtttggttgg ccaattaatt caactgtttg tgaatttcca
    40141 gtaccttcat aatcatttcc atcagcaaaa gctgtaaatg gaatcattac tgcacaaagc
    40201 gttagtaata aagcaattgt ttttttcatt tgttttcccc ctattttttt atattattct
    40261 caattacagt ttcctttctt tacgtgcctt aataatcata ttgtaattat gctcagcagt
    40321 ttcaaatgct cctaccaaag gagttataat tacactttct attaagcaca attcatcata
    40381 gtttaaacta gcttctccag catagaaatt atttaactta tctaaatcta ttctagttaa
    40441 aacggaaact gattccattg agaatttgta aacatcaata agtttatata atccacctat
    40501 atagtttttt gctacttcat ggttcttaaa aaattcatct tttcctgtat gtaggtttac
    40561 ttccataatt ccctccattg ttcttatatt tttaaccttc aaataatatt tacttcttct
    40621 tcaaaacaga atattctatc acggtagaat attctgtttt tttcattcta tcacgataga
    40681 ataatatttt caatcatttt ttctattcta ttttatttaa atagaataga aaaaaagaac
    40741 acttaatatt tttcaagcta atatattaag tgttcttttt atatttttac aagattatat
    40801 aaaattattc tttaatcaat taaagtgttt ttctatttct taataataag aaatgatata
    40861 aaattgtatc attttaaaga ttttgtgata taataaaagc aagaagaact acaatctatt
    40921 taggcgtaga gtggagttca taataaacgt agtttatttt ttgaatttaa atataaattt
    40981 aaattcaacc tgtaagtcac tctttgcacg agagtggctt tttgcttttt tgaatagttc
    41041 actgattaag taaactacca cactagctgt taaactagct aaaacattaa acaaaaagtt
    41101 atccatgtaa ctcacctccc ctcgaatcgt tgggaggata atcttttgta catgaactcc
    41161 actctataga ttgtatatta cgctcttctt gctaaattta attataccat attttgtaat
    41221 atcagttaag agtttgcact acaataatca gaaagcttgt tccactcaca atcaacccct
    41281 attattttaa gaatatcatt gacttggcta tttaaatctt taatatctga tttagacatt
    41341 aaaggatctt tcattatttc atttagaaaa atactatcaa tgattaaaca tacattttct
    41401 gataaatatt cacgttttac tgagattact gttccaactg tactttcttt tagtgtctta
    41461 tatgctacac tcaatatgca tggttctaaa ttaattcctt ctagtactgt ttcaaagtaa
    41521 tattttattc ttatattttc atttccaata aatatttctt cattgctgct tatcttcttt
    41581 aattttctca ttatgcctaa catcttagca ttcacttcat aatttattcc atatctcttt
    41641 gatgccaaac tcgacattaa gtctctttct aaataaatta cccactgacc attataggaa
    41701 aatgatgttt tagcgatatc catttctcct tttccagtaa tattaaattt aggctctagt
    41761 ttatttatta agtaaatctc ataaaaatcc atagtatatt tattatctaa ttccatatac
    41821 tcaagtctta aattcttatt acaccaattt tcttttttat tacttaaatg actagcgtgt
    41881 ctatcacaga tattctctgt tttcccaacg tataaacatt cttccgtatc ttgattaata
    41941 tatttataaa cataatacat tttttatttt ctccaattct taattgtcat atttacagcc
    42001 cttatatatt tcaaataatt cttgccatgt atatccaact tctagtatgt gaaaaatatc
    42061 ctctatttgt tggaaaatct cagtaatttt atattcaaat gttggcatta attccattaa
    42121 attggccaaa gatccaatcc taatctctaa tgttatattt ataagtattt ttttatttgt
    42181 tgctgtatat tctttccatc tgggataaac tgaaatcaaa gaacaacttc caaatctttc
    42241 taatgtttca aaatcatatg atatcaaatt aatatctaat tcattctcac aaaattctat
    42301 atccatattt tcaaagttat atagcacact taaaacatta ttttcatata acatttcaac
    42361 cttggcatct aacttttcaa ttctctttaa aaaattaata gattttttac ttacttcata
    42421 tttcatatct atctttcttt ctctgaatct tctttttccc tttgtctttt ccttaaaatc
    42481 ttcttctgaa tatgtactcc aatcttcttc cttgtaatca aaaaagatgt gttcagtatc
    42541 catatcacct ttacttagtt tattaaatat tggatttagt tcatttatta aataaacttc
    42601 aaaaaaatcc atattatatc tacgtggaag ttcaatatat tttaatatta attctttagt
    42661 acaccaatct tcttctttat tactcaaatg agatgaatgc cttgcatata tatcttctgt
    42721 ttttccaacg tataaacatt cttctgtagc tggatcaata tatttataaa cataatacat
    42781 tttttatttt ctccaatttt tatttatagt aatatccttc ttcatttact agaccttgat
    42841 caaataaatt tctcctaaca gtttcaagtc tgacaggatc tttgctataa tcaacttctc
    42901 tttcttcttt tttaacatct tcatcatccc aatatttatc taattcttct ttaaacttta
    42961 aatcatcttt ttgatgaatt ataacttcac ttattttatt tgaaagtgtt gatttaaaaa
    43021 aatcataatt ctttgtattt ataatatcag tattatctct ttcaaatgct atagcaatag
    43081 acttattaaa agcattcaga taaacatctt tcttaaaatc aaactcttta aaatcttctt
    43141 taaatagttt aatcgtccct gttgtaaaaa tattttcatt tggtatataa aaactaaaag
    43201 acacttcatt gtcctgcttc tttttaggta tataatcttc aaccaatata tctttatcaa
    43261 aatattttct aggttcataa tctatcacag aaaaaattat ttcatctatt tttctatttt
    43321 ttcttttttc ttcttttatt gaaacaacca tgttgccttt tttatttatt tcattcattg
    43381 ctctttttag aacattttgt ttaaaatatt tatactctgg atatacatta tcttttaatt
    43441 tcaaataaaa tcttaattca tctaacttat attttatctc cacttcctta ttttctctgc
    43501 tccatagtct aaataaagtg tataacctct gagtatatgc acctctaaaa ttaaataaca
    43561 ctgataaatt aatagctgta tatccatttt tctttttctg catttttaca aaatcagtta
    43621 tatgattgta tagaacttca tgcatcataa ttgtataaat ttggtctgta tgatccaatt
    43681 catatgtatt aattaaacca cttccaaaag tttttaactt gccagttgtc tcttctatat
    43741 agtcaaattc caaaacactt tgttgaaaca tgttcaaaat ttcttttata tttttatgct
    43801 cataatcatt attgtttttc ataaatactt ttagttcttc ttttgatata gttgtaacat
    43861 aagatgaatt ttgctgtttc tgagcattaa aaagtatttt gtaaaataat ttattttcca
    43921 catttgtaaa atcatattta ctttttatta aattatttgg ttgcattaaa atttcttttt
    43981 tttccaaaat ctatcacctc atataaatta taatacctaa tttataaaaa ggtcaactat
    44041 ttttgaaaga cgctcacctt tgacccttaa aatgatgacc tttgaccctt aaaatgatga
    44101 cctttgaccc ttaaaatgat gacctttcga cccttaaaat gatgaccttt gacccttaaa
    44161 atgatgacct ttgaccctta aaatgatgac ctttcatctc tgaaacctag atataccaat
    44221 tattatagct tgtctaaaaa ctattaaaga ctttaaaaac tgttttaaaa actattaaag
    44281 actagaaaag atgttctgaa ctattctttt tttatattaa aaaactttag tatttatttt
    44341 tctagtatac tttaaacact tactaaaaat aatttcttat aaaagattgg agatgaagaa
    44401 ttttttatgc ttgaaaacag aaaacaaatt cagaaagaaa tttacttacc aatagcaaca
    44461 tattgtttaa aagataagca atattatata aaagaatatg gagcactatt aataaagagt
    44521 gctgaaaata gtgacatttt taatacccat aaaaatagaa ttttgtataa gaataaactt
    44581 gatattcaag aaatagcaaa tgaactgaat gtaaataaag caacaatcca aagaaatata
    44641 aaaaaactag agaagttaga ttttaaaatt ctaaaaatag aaaataccct aaatggaatt
    44701 gtatattgtt tgccgtcaga aaataatatt gatttaaaca aatttgcatt gataaattat
    44761 gaaatgctta aaaaaatggt caataaattt aaatcaaata caataaaagt atatttttta
    44821 cttaaaacaa tcactacaga aactaatttt aaacctgcaa ctaatagttt tatagcagaa
    44881 aatataggac taagttctaa aagtaaaaat aatttagata tcataacttc ttctgttaaa
    44941 actttagaag aaaataaata cattgaaaca aaaaaagtaa atgtatatga atatgacaaa
    45001 gaaaaactta gagaagttcc aaaaataaga aaagtttatc ggatttgtaa ttttgatgag
    45061 tggaaaaagg aaattgataa aaataaagtt tattaaatat agcttaaaag catttaaagg
    45121 cttgtctgac gtgttttaat tacaaacttg atgttattta cctaaaaaga tacaaatttg
    45181 cttaaaaatg gaaatagaag gtcaaatata tagatagaga accaaataaa attatattgg
    45241 ctctctattt atcttcttcc caatcctcaa taaacttttc taaacttttt aatttatttt
    45301 cagacacttc ttcatcatca tgcagagcac ttataaaact ttcaaaagaa tttttgtgaa
    45361 agaaactaaa gaaactttta gtctcaactt ttaaatattc ttctttttct attaatgctt
    45421 tataatatgt aaatctatct ttcttaacaa cttctaaaaa tcctctacca actaaccttc
    45481 ttaaaaaagt taacatagtt gattttttcc attcatattt ttcacctaat gtttttaaaa
    45541 tttcagtaga tgcaacttct tcatcttcac tatcccaaat gtacatcatt accaaaagtt
    45601 ctgattctgg aatctttttt atcttcataa agcacaccac cttttcaaaa gtataactaa
    45661 aatactataa caatagagtt aactatacaa tactaaatgt ctaaaaatgt tattccaatt
    45721 tttaccaatt aatttttctt taaaaaaaat ttaagcagga gtaaaaaaaa taaaaaatta
    45781 tcaaaattta tcaatataaa aagaaatata agtataaatt tttctctttg tatattgatt
    45841 gtatacaatt ctatttttgt aaaattaaat tttacaaaat aatcaaaaat ggtaaatttt
    45901 gacatttgcc taatgtcaaa ttaggtgtta caattgtaac ataaagaaga tatatatcga
    45961 aataatacat ataaaataca tataaaacta cgtacgtaaa atatttttaa aaataatcaa
    46021 aaatcttaat tttcaatatt gaatacaatt aaattttcaa actcatctag ggcatgacat
    46081 acaaaagcaa aaaacattaa tttagtatct gataaaaatt taaaaattat aaaaggggga
    46141 agtctgttat ttttcagaag caatgtaact gtaaaaatat aaatcagaat attttgaaaa
    46201 tatgggggat tctatgggag aattaaaact tgaatcaaaa atgaattgca ctaaaaataa
    46261 gaaaaaagtt gctatatcta tacatcaaat gaaaaaaatt tctaaaaaag attatgtttt
    46321 tttaacagat tgcatagaaa cattttatat gaaaataaaa gaggtttgat aaattttcaa
    46381 acctcttttt attgctattt gtttttcttt tgcaataatt ctatcatttt caagatagca
    46441 tctctatctt cttgttctaa ttcataaagt ttgttagtta tttcttttac atcatcatca
    46501 atttctaagt cagctaaaca atctttataa atttcacctt ttccacttct tagccattcc
    46561 tcatttactt caaattctct gcatatgtcg ttaatacttc tttctgtcaa aactcttttt
    46621 cctttttcca ataaagaaat ttgatcttgt gataagaaaa ttctttcccc aaacgctctc
    46681 tgcgaaagtc tttcaagagt tcttaactca tttaatcttt ttccaatttc tttagctttt
    46741 aatttttttg aaacttctgc catttttaca atcctcctct gtcattacta tactataaaa
    46801 acacgacttt gtcattaaaa aaataattat aacgacaaaa tcatgataaa aataaaaaag
    46861 atattgattt ttaacgacaa agtagttata cttaaaataa gttgaacgac aaagtaagtg
    46921 taggaggata aaaaatgatt tgttcaaaca aagagaaagt agaaacagct ttgatgatag
    46981 attattt
//
LOCUS       DAGSWI010000012        46754 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.012, whole genome shotgun sequence.
ACCESSION   DAGSWI010000012 DAGSWI010000000
VERSION     DAGSWI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.012"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            70..795
                     /locus_tag="MJF80_000642"
     CDS             70..795
                     /locus_tag="MJF80_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBZ0279319.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            1260..4304
                     /locus_tag="MJF80_000643"
     CDS             1260..4304
                     /locus_tag="MJF80_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBZ0279320.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPTTLVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTASHNEISNNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYKCGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDNSGNTEVAEVKATTVYIKELTAPMGFKVDKT
                     IYSLKVETGKTATLKVSDTPKVTDTLIELFKIDMETQKANPQGNASLEGAEFTWKYYA
                     GFYTKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLTCEETKSPTGYLLDGAYMQANGSEEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETGDTKPQGSATLKDTAFDIISLNDNSVLVEGKLYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPNGYLTDGAKPIDFAITENGKIVDLTDEAHSV
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSSDWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEITIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAEIIIDGKRVESDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTKYKLVGWQMLKEENAELLINGKRVESDYTFIADSETMKVEVTFTFDATSL
                     DDKQLVTFEELYDLSNPNEPKKVTEHKDIEDEGQTITFKEKPEVPEKPETPTTPEKPS
                     RPTDSPKTGDNTNVMAFLVMLLTSAGGLAGTYLYKRRKMKKS"
     gene            4540..4866
                     /locus_tag="MJF80_000644"
     CDS             4540..4866
                     /locus_tag="MJF80_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBZ0279321.1"
                     /translation="MELKYVVPNMAQTFGNLEFAGENKIEQQRINGRFTPVSRSYNLY
                     SDVQRADDIIVTIPAKAGEKHFKPEQRVKLINPKITADGKAVKGRGFTNYILTADDML
                     PAEESK"
     gene            4882..5265
                     /locus_tag="MJF80_000645"
     CDS             4882..5265
                     /locus_tag="MJF80_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBZ0279322.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRAENDDGSTSEEITRRT
                     YDLKCSVQERMIQVSIPADIPVKDYPYNAEVELVNPVADTVANASYRGVNVDWYIKAD
                     DIILKNKGGQVGNPQNHAPQGQPKK"
     gene            5363..5848
                     /locus_tag="MJF80_000646"
     CDS             5363..5848
                     /locus_tag="MJF80_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279323.1"
                     /translation="MAKEKGSFKEFLSAIAPEHQVFVEKLNTKLIEQGCDLVIKEAKS
                     GYTASYQLEKKTVMNWVFRKTGVFARIYGDNAGKYEDIIASLPADMQKKMTTSRDCKR
                     LIDPNACSDTCVKGFVYALNGDTHKKCRNDGMLFVLTNETAEHIAGLVCAEVNVRKSA
                     L"
     gene            5965..7359
                     /locus_tag="MJF80_000647"
     CDS             5965..7359
                     /locus_tag="MJF80_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0279324.1"
                     /translation="MKMILNKGHRIRASDKNLVYHFSIGTLLFVFIAVILLLNMKQLM
                     RTDWEHFSLLDNGLTLSTYNFITLLIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIIWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTMLYDMIANRITIDEVKAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSKEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            7561..8760
                     /locus_tag="MJF80_000648"
     CDS             7561..8760
                     /locus_tag="MJF80_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBZ0279325.1"
                     /translation="MNETDFLTALKEKRCAYGVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPDNPMFLLFDYVRIRFPTMDIKHIIKDILKLNIDYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLVAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIQKCEHEECISKFRSFKSYGSGELVKHNETDKYD
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAELTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTKPESY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHYQIIKQQTATVEE
                     MVANVKE"
     gene            8768..8902
                     /locus_tag="MJF80_000649"
     CDS             8768..8902
                     /locus_tag="MJF80_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBZ0279326.1"
                     /translation="MWELLKDLLLVSFGIGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            8903..9124
                     /locus_tag="MJF80_000650"
     CDS             8903..9124
                     /locus_tag="MJF80_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279327.1"
                     /translation="MNFGQNLYTWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            9208..9738
                     /locus_tag="MJF80_000651"
     CDS             9208..9738
                     /locus_tag="MJF80_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0279328.1"
                     /translation="MCKILLVRPSKQYADQVMSYKEEMSQNGDSFDGCAGLENVQSFE
                     EWIDFEARLKAKYKDGYVPSEVFLAIRKKDNYVIGMIDFRHPLSDFLFNFGGNIGYSV
                     RPSERQKGYASEMLRLILPICHEFGESKVLLTCDKKNEASQKTIVRNGGVLEKEIPDT
                     VGLSESGIIQRYWISI"
     gene            9813..10355
                     /locus_tag="MJF80_000652"
     CDS             9813..10355
                     /locus_tag="MJF80_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBZ0279329.1"
                     /translation="MKGFERKNQLFSLCGLNCGLCPMLLGNHCGGCGNGNQSCKIARC
                     SLEHGMIEYCYECKQYPCEKYQHIDDYDIFITSRRQKADLERAKIIGIEQYNLEQQEK
                     TQILSYLLSNYNDGRRKTFFCVAVNLLELSELQEAMKQIQSNDELTLLPFKAQCLYVV
                     EMFQKIADRRNIKLKLIKKK"
     gene            10459..10755
                     /locus_tag="MJF80_000653"
     CDS             10459..10755
                     /locus_tag="MJF80_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBZ0279330.1"
                     /translation="MNDIFKDMQAKVGCENISDLPSYKRKVWHEMKQLNFADYEEKQL
                     EDFSNYVFGMSCQTLKDVMKQQKGREEQCRKQGCWWKRAEQLAKKQHHTGSTCR"
     gene            10674..11177
                     /locus_tag="MJF80_000654"
     CDS             10674..11177
                     /locus_tag="MJF80_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBZ0279331.1"
                     /translation="MQETRVLVETSGTTGEETTSYWFDLPIDVAEFEEKLGVGAESGD
                     YRIIEKVLPFADEVHEHTSVYQLNELDFMYRQLSSDMQEEYTALLTVYENLEALYICR
                     NVIAVYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFEAYASHLLENRRYLVTEH
                     AVFELPE"
     gene            11195..11698
                     /locus_tag="MJF80_000655"
     CDS             11195..11698
                     /locus_tag="MJF80_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBZ0279332.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQC
                     EEYAIHDTDNFPIEIGEYVSIEELNEMYEMIEELPDYIVECLDEFIGHYGTLEEVMEH
                     KDNIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            11808..12197
                     /locus_tag="MJF80_000656"
     CDS             11808..12197
                     /locus_tag="MJF80_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBZ0279333.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFFI
                     ILFGDMPPLSMVEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLDKQILNETITAVRSVNYVPD"
     gene            12184..14634
                     /locus_tag="MJF80_000657"
     CDS             12184..14634
                     /locus_tag="MJF80_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0279334.1"
                     /translation="MYPIKYIDNNLVWNKDNEVYAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLREVAVRKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIDDFGYLMEHLYGRDGIAYEDYEYQLPKKKYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKVVRSVTQSETRGLLHVIDELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFNEYTTIELLSVAMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPIQRNEVE"
     gene            14634..16820
                     /locus_tag="MJF80_000658"
     CDS             14634..16820
                     /locus_tag="MJF80_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBZ0279335.1"
                     /translation="MKERIQSAFTKKKIFHFLKMALFVVALSLILLSLLGTMAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTQNGFSSTGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNENNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYMAHRARRMERRLAGAVTAGGVAGAVAGSTISNGKPTRKTSTKDSRGNTSSSSMGQ
                     RAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVHSAKEKAKTSVSDFKRGVVQ
                     EQQSRQTGRSEKLEQHKKNIADKRMELQKAQEARQARKMDGSATTGATRSHERPATAL
                     KPSVEKTQEVKRPATTSTSKVSEPVKTNGVKERPLSSGASDRKTTQSAQPVYRQNIEK
                     VVSQETRQNYKTERTTKTQTFEQSKHTTEHTEKNRNLVTKKGQKKK"
     gene            16817..17821
                     /locus_tag="MJF80_000659"
     CDS             16817..17821
                     /locus_tag="MJF80_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBZ0279336.1"
                     /translation="MKLKHIAIIGSLFPILFSIVLFFGVLISADTDDDNSNFSSGITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGIDDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ENFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQISGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLVQWCYGKAGISLPRTAQTQYDATQHIPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYADLSSSYWQQHLIGAGRVK
                     "
     gene            17841..18743
                     /locus_tag="MJF80_000660"
     CDS             17841..18743
                     /locus_tag="MJF80_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBZ0279337.1"
                     /translation="MFKKNKKQTENIKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDMHTIHETETIQLRLTDTNGIENFVKNFAKSYYSWNNSKEAIDARTQAISG
                     YLTKELQDLNVDTIRTDIPTSSAVTDVIVWNIEQFGTDIFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDADGDMVIVQNPTLAPAIQKSDYEPKTPEADGKLDADTINDATAFLE
                     TFFKLYPTATEKELAYYVAGNVLEPIGRDYLYSELVNPILTKDGDNVKVKVAVKFLDK
                     QTKATQVSQYELVLHKDSNWKIVG"
     gene            18846..19661
                     /locus_tag="MJF80_000661"
     CDS             18846..19661
                     /locus_tag="MJF80_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBZ0279338.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALACSNDTIIEQLDKKSIEIMDNIQKEQEKLKKIE
                     IAKKELLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCTIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            19742..20218
                     /locus_tag="MJF80_000662"
     CDS             19742..20218
                     /locus_tag="MJF80_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBZ0279339.1"
                     /translation="MTTYKVNSIGTIYNNEKGAFIQLEAPYIPALKELDGFSHINVLW
                     WFSDFDNADFRSVLETDQPYKKAPEKMGIFATRSPVRPNPIALSAVEIINIDYMSGLI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPQVPDWCSHWPHSLEESASFAWENEFNF"
     gene            20232..20783
                     /locus_tag="MJF80_000663"
     CDS             20232..20783
                     /locus_tag="MJF80_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0279340.1"
                     /translation="MSLQDYIFEKPVIETEHLILRTMNKNDIDDLKEWISDISLYEYW
                     GKRPSKNEKNPELLFNKPEKATKSFHWGIVHKQDSKVIGELWIYLIENDRMAKVAFRL
                     SKQYQGNGLMSEVLKGVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRE
                     GKMVNTYCDYYLYGILKSDYIIS"
     gene            21050..21666
                     /locus_tag="MJF80_000664"
                     /pseudo
     CDS             21050..21666
                     /locus_tag="MJF80_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            21720..22574
                     /locus_tag="MJF80_000665"
     CDS             21720..22574
                     /locus_tag="MJF80_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBZ0279341.1"
                     /translation="MRTVKVGTHKKSVIALWVVLIASVSFGVYKNFTAIDMHTVHETE
                     TIQLRLNDTNGIENFVKNFCKSYYTWDNSKEAIEARAQAISNYLTKELQDLNLDTIRT
                     DIPTSSIVTNVLIWDIEQSGTDDFIATYEVDQQIKEGEQTTNVKATYTVKVYVDKDGD
                     MVIVQNPTLAPAMEKSDYEPKAQEADNSVDADTTADATAFLETFFKLYPTATETEIAY
                     YVVGNALEPINGDYLYSELVNPVFTADEDNIKVKVAVKFIDNQTKATQISQYELTLHK
                     DGNWKIIG"
     gene            22707..22946
                     /locus_tag="MJF80_000666"
     CDS             22707..22946
                     /locus_tag="MJF80_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBZ0279342.1"
                     /translation="MPMKKGQHIFGTVKVGEKGQIVIPKEARQIFDIKAGDTLLVLGD
                     EEQGIAIVKADVMKELAVKILKGLGKLSNGIDDEK"
     gene            22936..23553
                     /locus_tag="MJF80_000667"
     CDS             22936..23553
                     /locus_tag="MJF80_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBZ0279343.1"
                     /translation="MKNNIMLILPIAFILDLLIPFILAPTYRGYSHLTQVMSVLGNSK
                     APLHNVYNTWLVIFGMTLAVINFKVYAVVSKGSRLIAIILFIVLLIYAIGGCILSGLF
                     SVGEVKSLNTLSEKIHGYGSVVGFMCLSFAPLLLGIYAYKINDIKFSFFCIGCFILAI
                     LCFSCFVMGDKPNFRNTFLAFEGLWQRLSLLFMYFPLGCLILFET"
     gene            23667..23864
                     /locus_tag="MJF80_000668"
     CDS             23667..23864
                     /locus_tag="MJF80_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279344.1"
                     /translation="MLKQGAKLSGVPLSEIQENIEATIDEAMNSTDPEVQANFKKYFG
                     NKRPTPEEYIYTMTKKSRFKV"
     gene            23880..24349
                     /locus_tag="MJF80_000669"
                     /pseudo
     CDS             23880..24349
                     /locus_tag="MJF80_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892232.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            24562..25419
                     /locus_tag="MJF80_000670"
     CDS             24562..25419
                     /locus_tag="MJF80_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBZ0279345.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGVHYLSHGKSPKIKKNYIMEDMLHNVYDATTYGIETFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPAWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVASLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            25497..26078
                     /locus_tag="MJF80_000671"
     CDS             25497..26078
                     /locus_tag="MJF80_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0279346.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVIFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            26182..27027
                     /locus_tag="MJF80_000672"
     CDS             26182..27027
                     /locus_tag="MJF80_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279347.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            27024..27707
                     /locus_tag="MJF80_000673"
     CDS             27024..27707
                     /locus_tag="MJF80_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0279348.1"
                     /translation="MRLGRLIRGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSGSNHLLGITVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIVCFA
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVKHM
                     WKSLGGVKL"
     gene            27704..28402
                     /locus_tag="MJF80_000674"
     CDS             27704..28402
                     /locus_tag="MJF80_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0279349.1"
                     /translation="MKRILSSTIQVFRQIKSDPMMFAACFTPFVMGALIKLGIPFLER
                     ITDFSLRAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            28414..29325
                     /locus_tag="MJF80_000675"
     CDS             28414..29325
                     /locus_tag="MJF80_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBZ0279350.1"
                     /translation="MEKGRLLQNGKVNRAIQVSKITLFINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVLSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVPSGIALTAAVLSIVIKEWMYWFTRNAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALDIFKESINKMVDHS
                     CDNATETKIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            29377..29517
                     /locus_tag="MJF80_000676"
     CDS             29377..29517
                     /locus_tag="MJF80_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279351.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNRSKDSEDDK
                     MY"
     gene            29809..30435
                     /locus_tag="MJF80_000677"
     CDS             29809..30435
                     /locus_tag="MJF80_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0279352.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNVLYQKCLVNGPISYIEDIFNEMIKKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKSQLTTHVNQFVKKYITIEKVK"
     gene            30432..30521
                     /locus_tag="MJF80_000678"
     CDS             30432..30521
                     /locus_tag="MJF80_000678"
                     /inference="COORDINATES: protein motif:HMM:NF025568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279353.1"
                     /translation="MIIFCPECKHNFLISAEELKMNIVISHKY"
     gene            30555..31271
                     /locus_tag="MJF80_000679"
     CDS             30555..31271
                     /locus_tag="MJF80_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBZ0279354.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            31435..31626
                     /locus_tag="MJF80_000680"
     CDS             31435..31626
                     /locus_tag="MJF80_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBZ0279355.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            31650..31880
                     /locus_tag="MJF80_000681"
     CDS             31650..31880
                     /locus_tag="MJF80_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279356.1"
                     /translation="MIGLKRRNMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            32147..32566
                     /locus_tag="MJF80_000682"
     CDS             32147..32566
                     /locus_tag="MJF80_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBZ0279357.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            32569..32823
                     /locus_tag="MJF80_000683"
     CDS             32569..32823
                     /locus_tag="MJF80_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0279358.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLVFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            33196..33399
                     /locus_tag="MJF80_000684"
     CDS             33196..33399
                     /locus_tag="MJF80_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBZ0279359.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRRLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            33477..34667
                     /locus_tag="MJF80_000685"
     CDS             33477..34667
                     /locus_tag="MJF80_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBZ0279360.1"
                     /translation="MSKVKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPAGKRDDISLREKEKQIKKDLDDGIDTVGGKMTVCQLYEKKNNQRKNIKRN
                     TKIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFNFKLSDVLEDDTEAKVILTPEQEEKLLAFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            34818..36728
                     /gene="mngA"
                     /locus_tag="MJF80_000686"
     CDS             34818..36728
                     /gene="mngA"
                     /locus_tag="MJF80_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBZ0279361.1"
                     /translation="MIRNLVSFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEYLEAVM
                     YRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVELIFLIAV
                     PESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKEEIRIQ
                     NESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHTPDIIK
                     RADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKGDYIEK
                     SSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQELYNQEG
                     SWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGFLGGML
                     GGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAALNNGL
                     INWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAIFASVK
                     MVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVIPSLVA
                     GSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIISAILLI
                     ITRKQKNQRKIH"
     gene            36872..39487
                     /gene="mngB"
                     /locus_tag="MJF80_000687"
     CDS             36872..39487
                     /gene="mngB"
                     /locus_tag="MJF80_000687"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBZ0279362.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPDRKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLIAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            39766..40920
                     /locus_tag="MJF80_000688"
     CDS             39766..40920
                     /locus_tag="MJF80_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBZ0279363.1"
                     /translation="MKMLISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(41043..42956)
                     /locus_tag="MJF80_000689"
     CDS             complement(41043..42956)
                     /locus_tag="MJF80_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBZ0279364.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGAFILSSKKGYKVNLNTNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            43198..43515
                     /locus_tag="MJF80_000690"
     CDS             43198..43515
                     /locus_tag="MJF80_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0279365.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            43523..44770
                     /locus_tag="MJF80_000691"
     CDS             43523..44770
                     /locus_tag="MJF80_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBZ0279366.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMETMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            44799..45152
                     /locus_tag="MJF80_000692"
     CDS             44799..45152
                     /locus_tag="MJF80_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBZ0279367.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            45174..46619
                     /locus_tag="MJF80_000693"
     CDS             45174..46619
                     /locus_tag="MJF80_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBZ0279368.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
BASE COUNT        16318 a         7255 c         9670 g        13511 t
ORIGIN      
        1 attaacgaaa attttagcta atatttttga attttactta tgaataataa aatagatgga
       61 ggagaaacta tggaatttta tgaaagagca aaaaaatatg agtataaatt tacagataca
      121 gaagacatga taattgaata tatgagaaaa aacaaagaag aaatagtaaa attatcaata
      181 caatctttag cagataaatt ttttactgtc ccaaatacca tagttaggct atgtaaaaaa
      241 atggagtatg atggtttctc tcaactaaaa aataatttaa aagaagagtt aaaaaatgag
      301 gataaagagc caaaaaatgg ttttgaatca gatataaaaa agacattaga gctaatagat
      361 tatgaaaaaa taaataaagt agcagatata attcaaaaat ctaggagtat tatagtttat
      421 ggaataggtc aaaacattcc tttatgtgag atattagtaa aagaaattag aggtgggcat
      481 gataatataa cttactatag tcaaagacat gaagtacttc atgatatagg aaaattaggt
      541 gaacaagata tgttttttgc acttagttta agtggagaaa cgaaagaagt tatagaagca
      601 gcaaagacag cagttaataa gggttgtaag gtagtagctt taacacatat atgtgaaaat
      661 caattgcaac aaattgcaga tataagttta tattgctatt caacaattaa acaagtaaat
      721 gggtacaata tatcagataa ggttccaatt atgatgatta tgagagcaat aatagagcaa
      781 tattggttaa gatagagatt tgtgtataaa tacacaaatc tttatttttt aagaagaaat
      841 tgtgtgtata atttcaaaaa aacattttca taatttattt tatgctatat tcatagcatt
      901 aaatgattta aggagggcgt aattttggaa tttaagaaaa tcacaaatga aaaccttgtt
      961 tttttcctca aaaaaaacat actaaaaagt tgattatccg ctaaaacaca gtatctatca
     1021 gcattttcaa gaaaacaaac acatcaattt actactaatt tactaccaat gaatgagcct
     1081 tgatacgcta gttttcctag atatgcaaag atatggtacg gcacatcagt attgaagcaa
     1141 gaaaaaaatt gaatatttca tagactatgg aacgcctaaa atgacgttcc ttttctattt
     1201 ccagccgttc ttattgaacg gctattttat tacccaaaat gaaaggagca tttagaatta
     1261 tgaaaaagat attgaaacga ttgtgtacgg gcgtacttgc ctttgcgact gtcgttactg
     1321 ctttaccgac tacacttgtt catgcagaaa gtaagcaata ctggacggaa agtgaagaac
     1381 gtgttggtat cgttgaaaag gtaatgaatg acggttcgat tggttctacc tttaacgagg
     1441 ggcatttgac cgttgaaggc gaggacgctt actgtattga cattaacaca gattttaaga
     1501 acggctataa gaccagagct gacgcaagct cccgtatgag tgccgaccag ataagtgatg
     1561 ttgctttatc tatcgaatat gtgaagcaat acaccgccag ccacaatgag ataagcaaca
     1621 atcatgccta ccttttaaga cagcttgttg tctggcaaag attgagtgtt cacttaggct
     1681 ggcaatgtga taacgtgcga gcttcttatg atgaaattcc aaaggctacg caagatgaag
     1741 tgtttgctgg tgcaaaagcc tttgtcaaag aaaacaaagg acgctataaa tgtggcggtt
     1801 atatctattc tggcgagggg caggaattag ggcaattctg ggcgaagtta aatgttggaa
     1861 atgccaaact tcaaaaaact tccaataata ccagcattac agacggtaac gggaattact
     1921 ctatcgctgg tgcgacttac ggtgtctttt ctgataagga ctgcacaaaa cagcttgcta
     1981 cccttacgac cgacaacagc ggaaatacag aggttgcaga agtcaaagct actaccgtat
     2041 atatcaaaga attgactgca ccaatgggat ttaaagtgga taaaaccata tattccttaa
     2101 aagttgaaac tggaaagaca gcgactttga aagtatctga tacgccaaaa gttaccgata
     2161 ctttgattga gcttttcaag attgacatgg aaacacagaa agccaaccca caaggtaacg
     2221 cttctttaga gggtgcagag tttacatgga aatattatgc aggcttctac actaaagaca
     2281 atcttcctgc cgaagctact cgtacatggg ttacaaagac aatcgctgaa acagacagcg
     2341 acgggacaac ccactacatc acaaaattag cagacgcata caaagtatct ggcgacagtt
     2401 tctatatgca ggacggaaaa gcggttttac ctcttggtac actaacttgc gaagaaacaa
     2461 aatcaccaac gggctactta ttagacggtg catatatgca ggcaaacggt agtgaagaac
     2521 agataaaagg cttatacctt acacagatta ctgaggacgg cgaccttgcc gtattaactg
     2581 gaagtaacca gttttctgta tcagacaaag tcattcgtgg cggtgtcaaa attcagaaac
     2641 gagatttaga aacgggcgat accaaaccac aaggaagtgc aaccttgaaa gatactgcct
     2701 ttgacatcat ttccttaaat gataattctg ttttggttga gggcaagcta tacaagaaaa
     2761 atgaagtcgt aaaaacaatc catacggaca ttgagggtat cgcttctact tccgctgacc
     2821 ttttgcctta cggaaatttc cgtatcgttg agaaacaagc tccaaacggt tacttgacag
     2881 acggtgcgaa gccgattgat tttgcaatca cagaaaacgg aaaaatcgtg gacttaacag
     2941 acgaagccca ctctgtctac aatcagatta aacgtggaga tattgagggc gtgaaaatcg
     3001 gtgcaggcac acacaagcgt cttgctgatg ttccctttag gatcacaagc aagacaacgg
     3061 gcgaaagtca tgttgtggta actgatgata acgggcaatt ctccacttct tctgactggg
     3121 cttcacataa gcacaatacc aacgcaggaa agaccagcga ggacggtgtg tggtttggaa
     3181 cttctgaacc agacgacagc aaaggtgcat tgccttacga tacttatatt attgaagaat
     3241 tacgctctga cagcaacaaa ggttttgagc ttattccacc atttgaaatc gtggtatcaa
     3301 gaaataatct tgtgattgat ttaggaactt tgactgatga atacgaaaaa gaaatcacta
     3361 tccataccac agcaaccagc aaagacggcg aaaagacaat ccttgcagga aaagaggtaa
     3421 caatcgttga tactgtcaaa ttagacggac ttacaaaagg cactaagtat cagttaaaag
     3481 gctggcagat gttaaaggaa gaaaacgccg agattatcat tgacgggaaa cgtgtagaaa
     3541 gcgattatac ctttgttgct gatgatgaag aaatgaaagt ggaaatttct tatacattca
     3601 atgcgtccga gcttggtggt aaaaatctgg taacattcga ggaattatac gacttaagca
     3661 atccagacga acccgtgaaa gttgcggagc ataaagatat tgaggacgac gggcagacgg
     3721 tacttattac agaacgtatc atcaaaatac atacaactgc taccgataag gacggtaaca
     3781 aagagcttga agcaggaaaa gacgtaacaa tcattgatac tgtaacctta gagggcttgg
     3841 aagttggtac aaagtataaa cttgtgggct ggcagatgtt gaaagaagaa aatgcagagc
     3901 ttcttatcaa tggaaaacgt gtggaaagtg attatacgtt tatcgctgac agcgaaacta
     3961 tgaaagtgga agttaccttt acctttgacg ctacttctct tgacgacaaa cagcttgtaa
     4021 cttttgaaga attgtatgat ttaagcaatc caaacgagcc gaaaaaagtt actgagcata
     4081 aggacattga ggacgaggga cagacgatta cttttaagga aaaaccagaa gtcccagaga
     4141 aacccgaaac gccaacaaca ccagaaaaac cgagcagacc aacagacagt cctaaaacgg
     4201 gcgacaacac aaatgtcatg gcatttctgg taatgttgct tacatctgct ggtggactgg
     4261 ctggaacata tctttacaaa cgccgtaaaa tgaagaaatc ataaggcggt aacggtatga
     4321 gaaaagaaaa atcacgctct ccgccgaagc tgtcaaacgg cagacgttta cttattctgg
     4381 cttatctgcc aacaacctgc aagcacacgg atagtcaagg gtgcgaagca ttgatttacc
     4441 ctttactatc tgggggattg ctggtatttc cctaattgcc agaataagaa agcacaatca
     4501 acaaacttaa aaaagaagaa agaaacgagg taaaacacta tggaattaaa atatgtagtg
     4561 ccgaacatgg cgcagacttt tggaaacttg gaatttgctg gcgaaaacaa gattgaacag
     4621 caacgcatta acggacgctt tacgcccgta tcaagaagct acaacttgta ctctgacgta
     4681 caaagggctg atgatattat cgtaaccatt ccagcgaaag caggcgaaaa gcatttcaaa
     4741 ccagaacagc gagtaaaact tatcaatcca aagattaccg cagacggtaa agcggtcaaa
     4801 ggacgagggt tcacaaacta tatcttaacg gctgatgata tgttgccagc cgaggaaagc
     4861 aagtaagggg gggaaatgat tatgagatta gcaaacggta ttgtattaga caaggaaaaa
     4921 actttcggtg tattgaagtt ctctgccctc agacatgagg tgcgagcaga aaatgacgac
     4981 ggttcaacaa gtgaagaaat cacacgaaga acctatgatt tgaaatgtag tgtgcaggaa
     5041 cgtatgatac aagtatcaat tcctgctgat attcccgtca aggactatcc gtataatgca
     5101 gaggtagagc ttgtcaatcc cgtagccgat acggtagcaa acgccagcta tcgaggtgtc
     5161 aatgtggact ggtatatcaa ggctgacgat attatcttga agaataaagg cggtcaagta
     5221 ggcaatccac agaaccatgc accgcagggg caaccaaaga aataagctgg caaactagat
     5281 tttcattgta gcggatataa gcaactgtta taatatgtgt atgaagaaac ggataaattg
     5341 aaatttgtgg aggaaaaaga taatggcaaa agaaaaaggt tcattcaaag aatttctatc
     5401 ggcaatcgcg ccagaacacc aagtgtttgt cgagaaactt aataccaaac ttattgagca
     5461 gggctgtgac cttgtaataa aggaagcaaa aagcggttac accgcttcat accaactaga
     5521 gaagaaaaca gtaatgaatt gggtgtttcg caagacaggt gtttttgcac ggatttacgg
     5581 agacaacgct ggaaagtatg aggatataat cgcttcgctt cctgctgata tgcaaaagaa
     5641 aatgactact tctcgtgact gcaagagact gattgaccct aatgcctgta gtgatacctg
     5701 tgtgaaaggt tttgtttatg ccttgaatgg cgacacacat aaaaaatgcc gtaatgacgg
     5761 aatgctcttt gtactgacaa atgaaacagc ggagcatata gctgggctgg tctgtgcaga
     5821 ggttaatgtg cgtaagtcag ctttataacg acagaaaagt taagaacaat aaatcccagt
     5881 ttgacaactt catatccata cacacaaagc agttagtctt aggattgact gcttttttcg
     5941 tacccagaaa ggagtgattg atttatgaaa atgattttaa acaaaggaca ccgtatcaga
     6001 gccagcgaca agaaccttgt ctaccacttt tccataggga cgcttctgtt tgtattcatt
     6061 gcggttatcc tgctactgaa tatgaaacag ctcatgcgta ccgattggga gcatttcagc
     6121 ttattagaca acggtttgac gctttccact tacaacttca taaccctact gatagcgact
     6181 ggtgtttgtg cattggtcgc ttttctgtat taccgcttct gttatgacag tttcaagaag
     6241 ctcctgcacc gtcaaaagct ggcacgaatg atactggaaa acaagtggta tgaagctgat
     6301 accgtacaag acagcggttt ttttactgac ctgcaaagca gatcaaggga aaaaatcatc
     6361 tggtttccaa agatttatta tcaaatggaa aaaggactgc ttcatatccg ctgtgaaatt
     6421 acattaggaa aatatcaaga ccagctctta cggttagagg ataaattgga aagtggcttg
     6481 tattgtgagc tgaccgacaa gacgttacat gacggttata tcgaatacac catgctttat
     6541 gacatgatag cgaaccgcat taccattgat gaagtgaaag cagaaaacgg ctgtcttagg
     6601 ctgatgaaaa atcttgtatg ggaatatgac gcactccctc acgctctgat tgctggtggg
     6661 actggtggcg gtaaaacgta ttttctgctg acgcttattg aagctctgtt acatacgaac
     6721 gctgttcttt acatattaga cccgaagaac gctgaccttg cagatttagg tacggtcatg
     6781 ggaaatgtgt atcacaccaa agaagaaatg attgattgtg tcaatgcctt ttatgagggc
     6841 atggtacaaa gaagcaaaga aatgaagcga cacccagact ataaaacggg cgaaaactat
     6901 gcctatcttg gtttaccgcc ctgctttctt atctttgatg aatatgtggc gttttttgaa
     6961 atgctgggga caaaagaaag tgtgagccta cttagccagc taaagaaaat cgtcatgtta
     7021 ggacgacaag caggatattt ccttattgtt gcctgccagc gtccagacgc aaaatatttc
     7081 tcggacggta tcagagataa ttttaacttc cgtgttggct tggggcgtat cagcgaattg
     7141 ggttacggta tgctgttcgg ctctgatgtg aaaaagcagt ttttccagaa gcgtatcaag
     7201 ggacgtggct attgtgatgt gggaacaagt gtcataagtg aattttacac gcctttagtc
     7261 ccgaaaggac atgatttttt acagactatc ggctctcttg catacgcaag gcaggacggg
     7321 acggcgacgt gcgaagcgaa aggcgacggc acggactagc cgttgctggt gtggcgttag
     7381 ccacgccagc agatacaagc ccctcattat ctaacagagg ggcacaatta cggcggaaac
     7441 ttccacaaaa acctttgcaa gcccgcataa aataagggtt tttcgtacat ttcgcctttt
     7501 gtgataaagc cttgaaatta tcacaatatc acaaaattat cacgattggg ggtatgacaa
     7561 ctgaatgaaa ctgatttttt aaccgccttg aaagaaaaac gctgtgcata cggcgtatcg
     7621 cagacaaggc ttgcaatcat ggcaaatatc agccgtgaac atttgagccg tatcgaagca
     7681 ggaaaggtaa cgctgaccga ggacatgaaa catacgcttt tagaagcggt ggaaaaattc
     7741 aatcccgaca atcccatgtt cctgctgttt gattatgtgc gtatccgttt tcccacaatg
     7801 gatataaaac atatcatcaa ggatatatta aagctcaata tcgactatat gcttcacgaa
     7861 gattacggac attacaaata caccgagcat taccatatcg gagatgtgtt tgtgtatgta
     7921 tcgcaggacg aggaaaaagg tgtcctgctg gaactgaaag gcaaaggctg tcgccagttt
     7981 gaaagctacc ttgtggcaca agagcgaagc tggtatgact tcttcacgga cgcattggtt
     8041 gagggcggtg tgatgaagcg tcttgatttg gcaatcaatg acagaacggg attgctggat
     8101 ataccagagc tgatacaaaa gtgtgaacat gaggaatgta tttctaaatt ccgttctttc
     8161 aaaagctatg gttctgggga actggtaaag cataatgaaa cggataaata cgatatggga
     8221 cacacgctgt atatcggttc attttcaagt gaagtgtact tctgttgtta tgaaaagaat
     8281 tacgaacagt acgccaaact tggtataccg attgaggaag tccccataaa aaatagattt
     8341 gagatacgtt tgaaaaatga acgtgcctat tatgctgttg tggaactgct gacacattat
     8401 gacgcagaac taacagcatt ttctattatc aatcaatata tccgcttcgc tgataaagaa
     8461 ccagacaagc gaaaaagcga ctggaaaacc aatgtacgct gggcttggtt cattggcgag
     8521 gggcgaccgc ccgtaaagct gacgacaaaa ccagaatctt acacattgga gcgtacactc
     8581 aactggctac aaaggcaggt tgcaccaacc ttgaagatgt tgaaacagat agacaaggac
     8641 aatcagacgg agtatctgga aacgattgaa aagcaggcaa aactgaccga acgacactac
     8701 cagataatca agcaacagac agccacggtt gaagaaatgg ttgccaacgt aaaggagtga
     8761 tgaaaaaatg tgggaattat tgaaagactt attgctggta tcatttggaa taggtatcgg
     8821 cgtagtctta atgtgcattt taactgtcgg caaagaagct gacagatgta tggaagaaat
     8881 caaagaaaga gaggacaatt aaatgaattt cggacaaaat ttatacacat ggtttttaag
     8941 taacgcccag agcttagtgc ttatggcgat tgtggttatt ggtatctact tagggttcaa
     9001 acgtgagttt tcaaaactta tcggcttctt ggtagttgcc ttgattgctg tcggtttggt
     9061 attcaatgct ggcggtgtga aagacgtatt gttagagctg ttcaataaga ttatcggtgc
     9121 gtaaaatttt attgtggtgc tgatatgaga atgctataat gtggatatga agaaagcgac
     9181 aaatgggaat tttgggggag aagtaagatg tgtaaaatat tgttagtcag accgtctaaa
     9241 caatatgcag accaagttat gtcatataaa gaagaaatgt cacaaaatgg agatagtttt
     9301 gacggttgtg ctggtcttga aaatgtacaa tcatttgagg aatggattga ttttgaagca
     9361 agattaaaag caaaatataa ggacggatat gttccatcag aagtgttttt ggctatacga
     9421 aaaaaagata actatgttat tggcatgatt gattttcgac acccattatc cgacttcctc
     9481 tttaattttg gtggtaatat cggatatagt gttcgtccat cggaacggca gaaaggttat
     9541 gcgtccgaaa tgttaagatt gattttacct atctgtcatg agtttgggga aagcaaagtt
     9601 ttactaacat gtgataaaaa gaatgaagca tcacaaaaaa cgattgtaag aaatggtggc
     9661 gtgttggaaa aagaaatccc tgatacagta ggacttagtg aaagtggaat tatccaacga
     9721 tactggattt caatatagac tctctttcat caaatccagt ttgtaaaagt caataaatac
     9781 aaagataaat ttgaagttag gagactaata ctatgaaagg atttgaacgc aaaaaccaat
     9841 tattctctct ttgtggattg aattgtgggt tgtgtcctat gcttttagga aatcattgtg
     9901 gcggttgtgg caatggtaat caatcatgta aaattgcaag atgtagtctt gaacatggaa
     9961 tgattgaata ttgctatgag tgcaaacaat atccgtgtga aaaatatcaa cacattgatg
    10021 attatgatat ttttattacg agcagacgac aaaaagcaga tttagaaaga gcgaaaatca
    10081 taggcattga gcaatataat cttgaacagc aggaaaaaac acaaattctt tcttacttac
    10141 tttctaacta taatgacggg cgtagaaaga ctttcttttg tgtagcagtc aatttattag
    10201 aactttcaga attgcaagaa gcaatgaaac aaatacaatc aaatgacgaa ttgaccttac
    10261 taccatttaa ggcacaatgc ttgtatgtgg tagagatgtt tcagaagatt gccgacagaa
    10321 gaaatataaa actgaaactt atcaagaaaa agtgattttt aaatttcagt ttgacaactt
    10381 catatccata cacacaaagc agttagtctt aagatcgact gcttttttct tactttaatt
    10441 tcagattgga gtgatgaaat gaacgatatt tttaaggata tgcaggcaaa ggttggctgt
    10501 gaaaacattt cagacctgcc ctcttacaaa cgtaaagtgt ggcatgaaat gaagcaactg
    10561 aactttgctg actatgaaga aaaacagtta gaagattttt ctaactatgt gtttggtatg
    10621 tcatgccaga ccttaaaaga tgtgatgaaa caacagaaag gacgtgagga acaatgcagg
    10681 aaacaagggt gctggtggaa acgagcggaa caactggcga agaaacaaca tcatactggt
    10741 tcgacctgcc gatagatgtt gccgagtttg aagaaaagtt aggtgtcggt gcagaaagtg
    10801 gggattaccg cattattgaa aaggtactgc cctttgctga tgaagtccac gaacatacaa
    10861 gcgtgtacca gctcaacgaa ttagatttta tgtaccgcca gctttcaagc gatatgcagg
    10921 aagaatatac agcacttctt accgtatatg agaacttaga agcactttat atttgcagga
    10981 acgtgattgc ggtttatccc gactgcaaaa gcatgataga tgttgcaaga caaaagctga
    11041 tgaacgaccc gacgttcaaa catttatccg aggactgtca agaatactac tttgactttg
    11101 aagcctatgc ttctcactta ctggaaaaca gacgctatct tgtaacggaa cacgctgtct
    11161 ttgaactgcc agagtaggaa atgaggtggt acttatgatt gatgatatgg cagtttatat
    11221 cgctaatctt ggcaagtaca atgagggcta tttagtcggt gcttggttca ctttccctat
    11281 tgacgaggaa gatgtgaaag aaaaaattgg tttgaatgaa caatgcgaag aatacgcaat
    11341 ccatgatacc gacaacttcc ctattgagat tggggagtac gtttccattg aagaactcaa
    11401 tgaaatgtat gaaatgatag aggaacttcc cgactacatc gtagagtgtt tggacgaatt
    11461 tatcggtcat tacgggacgc tggaagaagt catggaacac aaggacaata tttattatta
    11521 tcccgactgt gaaaccatga cagacgttgc ctactactac atagacgaat tgcaggcact
    11581 tggagacatt ccacccagct tgcagaacta tatcgactat gaagcctatg ggcgagattt
    11641 agatatgggc ggttgcttca ttgcaacaag ccgaggtatg tgcgagatac catattaacg
    11701 ctggaagatc agcgacaagt attgttgcta ctgacagcgt atttctcttt taagggacag
    11761 tctgaaacat ggctgtcctt ttccattttg aaaggagttg aaagaacttg aaaaagatta
    11821 aaagctacac gggtatctgg aatgtagaaa aagtcttata tgcaatcaat gactttaacc
    11881 taccttttcc agttaccttt acacagatta catggtttgt gattacagag tttttcatca
    11941 ttctgtttgg ggatatgcca ccactttcca tggttgaggg agcattttta aaatacttcg
    12001 gtattcccgt tgctcttact tggtttatgt cccagaaaac gtttgacgga aagaagccgt
    12061 acagtttttt gaaatcacag ataacctatg ccctgcgacc caaaatcact tatgcaggaa
    12121 aagccgtaaa actggataag cagatattaa acgaaacgat tacggcagta aggagtgtga
    12181 actatgtacc cgattaaata tatcgacaac aatcttgtct ggaacaagga caatgaagtg
    12241 tacgcctact atgagctgat accttacaat tacagctttc tatctgcgga acagaaattt
    12301 attgtgcatg acagctttcg ccagctaatt gcacagtccc gtgagggtaa aatccatgcc
    12361 ttgcagattg ctacagaaag ctcaattaga agtatgcagg aacagtccaa acgattggtt
    12421 acggggaaac ttcgtgaggt ggcggtaaga aaaattgatg aacagaccga agcgttagta
    12481 tctatgattg gggacaatca agtggactac cgcttttttc ttggcttcaa gctcatggta
    12541 acggaagaac agctcaatct gaagaacatc aaaaaatcgg cttggctgac attcaaagag
    12601 tttctccatg aagtgaacca cacgctgatg aatgactttg tttccatgcc gaatgatgaa
    12661 atcaaccgct acatgaaaat ggagaagctg ttggaaaata aaatctcaag gcgtttcaaa
    12721 gtacgccgat tagaaatcga tgattttggc tatctcatgg aacatctcta cggcagggac
    12781 ggtattgcct atgaagatta tgagtatcag ctaccaaaga agaaatacaa aaaagaaacg
    12841 ctgataaaat actatgacct tatccgtccg accagatgtg tgattgagga aagccagcga
    12901 tacttacgac tggaacatga ggacaaggaa agctatgtgt cctactttac cgtcaatgcg
    12961 attgtggggg aacttgattt tccgtcctct gaaatctttt atttccagca acagcaattc
    13021 acattccccg ttgatacgag catgaatgta gaaatcgtgg aaaatagaaa agctcttacc
    13081 acagtacgca acaagaaaaa ggaattgaaa gaccttgaca atcacgccta tcaagcagga
    13141 agtgaaacaa gctcaaatgt agtggacgca ttagacagcg tggacgagct ggaaactgat
    13201 ttagaccaga ccaaagaaag tatgtataag ctaagctatg ttatacgggt gtctgcaccc
    13261 gatcttgacg agctgaaacg ccgttgtgat gaagtcaagg acttttacga cgacctcaat
    13321 gtaaagctgg tgcgtcctgc tggggatatg ctggggcttc attctgaatt tttacctgcc
    13381 agcaagcgat atatcaatga ctatgtgcag tatgtaaaat cagattttct agctgggctt
    13441 ggttttggtg cgacacagca gttaggggaa actacgggta tctatatggg ctattctgtt
    13501 gatacgggaa gaaatgtgta cctgcaaccg tcccttgcca gtcaaggcgt aaaaggaacg
    13561 gttacaaatg cccttgcttc tgcctttgtg ggttcgcttg gcggtggaaa gtcgttctgt
    13621 aacaacctgc ttgtgtatta ctctgttctc tttgggggac aagcggttat cctagacccg
    13681 aaatcagagc gtggcaactg gaaagaaaca cttccagaga ttgcacatga aatcaatatc
    13741 gtaaacctta ccagcgataa ggacaacgca ggacttcttg acccgttcgt gattatgaag
    13801 aatgtaaaag acgctgaaag tcttgcgatt gatatactga cattccttac gggtatttct
    13861 tctagggacg gcgaaaaatt ccccgtactt cgtaaggtgg tgcgttctgt tacccagagt
    13921 gaaacaaggg gcttgcttca tgtgatagac gaacttagaa aggaagatac cgcaatctca
    13981 cgaaatatcg cagaccatat cgacagtttt acagactatg actttgcaca tttattattt
    14041 tcggacggaa cggtggaaaa tgctatcagc cttgacaacc agctcaatat cattcaagta
    14101 gcagatttgg tattgccaga taaagatact acttttaacg aatacacgac cattgaatta
    14161 ttatcggtgg caatgttgat tgttattagt acctttgccc ttgattttat ccattcggac
    14221 agaagcattt ttaagattgt tgatttggac gaagcgtggg cgttcttgaa cgtggcacaa
    14281 ggcgaaacct tatctaacaa gctggtacgt gctgggcgag ctatgcaggc aggcgtttat
    14341 tttgttaccc agtctgccta tgatgtttca aaggaaagtt tgaaaaataa tatcggctta
    14401 aaattcgctt tccgttctac tgacatcaac gagataaagc agaccttaga atttttcggt
    14461 atcgacaagg acgacgaaaa caaccagaaa cgcttacgag atttggagaa cggacaatgt
    14521 ttattgcagg acttatacgg gcgtgtcggt gtggtgcaga tacacccagt ctttgaagaa
    14581 ctcttacatg cctttgatac cagaccgccc atacagagaa atgaggtgga gtgatgaaag
    14641 aaaggataca gagtgcgttc acaaaaaaga agattttcca ctttctcaaa atggctctat
    14701 ttgtcgtggc actttccctt atcctgcttt cacttcttgg gacgatggct catgcaacgg
    14761 gacttgtgga cgataccata aatgcggaaa atctttactc aaaatatcct ctttccaact
    14821 accagcttga tttttatgtg gataatagct ggtcgtggtt gccgtggaac tggctggacg
    14881 gtatcggaaa atcggtacag tacgggcttt actgtatcac taattttgtc tggacgataa
    14941 gcctttactt atctaatgcc acgggctatg tggtgcagga agcctataaa cttgatttta
    15001 ttaacgatat ggcagacagt atcggaaaaa gcatacagac acttgcaggc gtaacacaga
    15061 acggtttttc ttctacgggc ttctatgtgg gcttcctgct actcattatc ttagtggtgg
    15121 gactatatgt tgcttacacg gggctgataa aaagagaaac cagcaaagca cttcacgctg
    15181 ttatcaactt tgttgtggtg tttgtattgt cggcttcatt tattgcctat gctcccgatt
    15241 acatcaaaaa gataaatgaa ttttcatcag acatcagcac cgcttctttg gacttgggaa
    15301 caaaaatcat gctccccaat tccgacagcg agggcaagga cagcgtggac ttgataaggg
    15361 acagcctatt ctctattcaa gtggaacagc cgtggctact cttacagttt ggaaacagca
    15421 atgcagaaga aatcgggaca gaccgtgtag aagctcttgt atcggcaagc ccagaggacg
    15481 aggacgggaa aaccagagag gaagtcgtaa aaacagaaat cgaggataac gagaacaaca
    15541 atctgaccat accgcaggtg gtaaatcgtt taggtatggt gttcttcctc ttgtttttca
    15601 acttgggtat cacgatattt gtattcttgc ttacgggcat gatgttattt agccagatac
    15661 tttttattat tttcgctatg ttcctgccta ttagtttcct gctctctatg ataccgagct
    15721 atgaaagcat ggcaaagcag gcaatcgtga gagtatttaa taccataatg acaagggctg
    15781 gaattacgct tattgtaacg gtggcgttca gtatttccag tatgttttat aacatatcca
    15841 cggactatcc gttctttatg gtggcgttct tacagatagt gtgtttcgct ggtatctaca
    15901 tgaaactggg cgatttaatg agtatgttct ccttgaacgc taacgacagt caaagcatgg
    15961 gacgaagaat attccgcaga ccatatttat atatggcaca tagggctagg cgtatggaaa
    16021 gacggcttgc tggtgctgtt accgctggcg gtgtggctgg tgcggtggca ggtagtacaa
    16081 tctcaaatgg aaagccgaca aggaaaactt ccacaaaaga cagtcgggga aatacatctt
    16141 cttccagtat gggacagcgt gcaggttcaa aggtgggtgc tgtcttagat acgaaaaata
    16201 aagtcaagga caaggcaaac gctgtcaaag aaaatattaa ggatatgccg acacagaccg
    16261 cttatgccgt ccattctgca aaggaaaaag caaaaacaag cgtgtcggac ttcaagcgtg
    16321 gcgtggtgca ggaacaacag tccagacaga cgggacgctc tgaaaaactg gaacagcata
    16381 agaagaatat cgctgacaag cgtatggaac ttcaaaaagc acaagaagca aggcaggcac
    16441 gaaagatgga cggatcagcg acaacgggag ctacccgttc ccatgagcga ccagctacag
    16501 ctttaaagcc gagtgttgag aaaacacagg aagtcaaacg acctgctaca acttcaactt
    16561 ccaaagtaag tgagccagtc aaaaccaatg gtgtgaaaga acgtcctcta tcttctggtg
    16621 cttctgatag aaaaacgacc cagtcggcac agccagtata taggcagaat atagaaaaag
    16681 tggtatcgca ggaaacacgc cagaattaca aaacagagcg taccacaaag acacagacct
    16741 ttgaacaatc aaaacataca acagaacata ctgaaaagaa ccgtaacctt gtaacgaaga
    16801 aaggacagaa gaaaaaatga aattgaaaca tatcgctatt attggcagtc tgtttcctat
    16861 tctcttttcc attgtccttt tctttggggt gctgattagt gctgacaccg atgacgataa
    16921 cagcaacttt tcttcgggca ttacgggtat gaacttatcc gcagaagttt tgaaacatca
    16981 gcctatggta gaaaagtacg ccagagaaaa cggtatctcc gagtatgtca atgtgttact
    17041 ggctatcatt caagtagaaa gtggcggtac ggcagaagat gttatgcaga gttcagaaag
    17101 tcttggttta ccgcctaatt ccttagatac ggaaagctca atcaagcagg ggtgtaaata
    17161 ttttgcgtcc ctgctttctt cctgcaaaaa tcaaggtatc gacgacttga atgtagcgat
    17221 acagtcctat aactatggag gtggctatgt gggatatgta gcaggaaaag gaaagaaaca
    17281 tacttttaac cttgcagaga acttcgcccg tgaaaaatcg ggcggtaaga aagtaaccta
    17341 caccaacccg atagccgttg cgaaaaatgg tggctggcgg tatggctatg gaaatatgtt
    17401 ttatgtggaa ttagtcaatc agtatttaac tgtaccgcag atatcgggag aactggcaca
    17461 aaaggtaatg aatgaagcgt tgaaatatca aggctggaaa tatgtgtatg gcggtagcaa
    17521 tcccaacact tcctttgact gttcaggact tgtgcaatgg tgctatggaa aagccggtat
    17581 ttccttaccg agaacggctc aaacgcagta tgacgctacc cagcatatcc ccttatcgca
    17641 ggcaaaggct ggggacttgg tatttttcca ttcgacctat aacgctggtt cgtatgttac
    17701 ccatgtggga atatatgtgg gaaataatca gatgtatcat gcaggcgacc cgataggata
    17761 tgcagaccta agtagtagct actggcaaca acacttaatt ggtgcaggac gagtaaaata
    17821 aaagaaagga cttagaaaat atgtttaaga agaataagaa acagacagaa aatatcaaag
    17881 aaaaaaaggt gcgtactgtc aaggtaggca cacataagaa aaccgtgatt gcgttgtggg
    17941 tggtgcttat cgcaagcgtg agctttggcg tatataaaaa ttttaccgct attgatatgc
    18001 acaccataca tgaaacagaa accatacaat taagacttac tgataccaac gggattgaaa
    18061 atttcgtgaa gaactttgcg aagtcctatt attcatggaa taacagcaaa gaagccattg
    18121 acgcaaggac gcaggcaatc agcggttatc tgacaaagga attgcaggac ttgaatgttg
    18181 atacaatcag aacggatata ccgaccagct ctgccgttac agatgtgatt gtgtggaata
    18241 ttgaacagtt tggaacggac attttttctg ctacctacga agtagatcag cagataaaag
    18301 agggagaaca gacaacagca gtcaaggcaa cttataccgt aaaagtccat gtagacgctg
    18361 acggggatat ggtaatcgtg cagaacccta cccttgcacc agcaatccag aaatcagact
    18421 atgaaccaaa gacaccagaa gcagacggta aactggacgc tgataccata aatgacgcaa
    18481 ccgcatttct ggaaacattc tttaagctgt acccgacagc aacagaaaaa gaacttgctt
    18541 actatgtagc tggaaatgtg cttgagccta tcggcaggga ctatctttat tctgaattgg
    18601 taaaccctat cttaactaaa gacggggaca atgtgaaagt caaggtggct gtgaaattcc
    18661 ttgataagca gacaaaggca acgcaggttt cgcagtatga gcttgtgcta cataaggata
    18721 gcaactggaa aattgtagga taacatggaa atgggcttgt atcataatta tggtatgcaa
    18781 gcccttttta tattgaagta taaattaaca ataattataa ttaggtacat gaatggaggg
    18841 ataaaatgtt caaaattggt gagttttcaa aattgacaca agtttctact cgtatgttgc
    18901 gttattatga tgaaacaggt ttattaaaac ctgctaaaat tgattcatta actggatatc
    18961 gtatgtattc agcagaacaa atctcaactt taaatagaat tatatattta cgtgatagtg
    19021 gtttcaatgt cgctgaaatt gcattagccc tcgcttgcag taatgatacc attatagaac
    19081 aacttgataa aaagtctatt gaaattatgg ataatataca aaaagaacaa gaaaaactaa
    19141 agaaaattga gattgctaaa aaagaattgt tgcatggcaa aagtgaattg tattttaaca
    19201 taaccataaa agagataccg agttattgcg ttttatcttt aagaaaagaa ataccaaatt
    19261 attattatga gggcgaactc tggaaagaac tctctgcttt tgctaaagaa aataaaataa
    19321 atatttctga tgaaacattc tcaatatatc acaatgaaga atataaagaa aaagatgttg
    19381 atgtagaatt atgtaccatt gtaaataaaa taggaaaagc tacaggagat tttaagtttc
    19441 gttttaccga accagtttct atgatggctt gtactatggt atatggagat ttttctaata
    19501 tttcttatgc ttatcgttca tttgcaaaat ggcttcaaga aaacagtcaa tataagatga
    19561 aaggttcaag ccgtcaaata gttcatcgtg gaccgtggaa cgagaaaaat ccagagaact
    19621 atctaacaga aatccaaatt cctttagaaa atctcttgta aaaaatgata atctgccttg
    19681 actttaacat ggtgttaagg tttatcgttt atttatcaag aagaaaacgg aggaaaatat
    19741 aatgacaact tataaagtaa attcaatagg tactatctac aacaatgaaa aaggtgcttt
    19801 cattcaacta gaagcaccat atattccagc attaaaggaa ttagacggat tcagtcatat
    19861 caatgtttta tggtggttca gcgattttga caatgcggat tttagaagcg tattggaaac
    19921 agaccaacct tataaaaaag ctccagagaa aatggggatt tttgcaacaa ggtcgccagt
    19981 acgcccaaat ccgattgctt tatcagcagt agaaattatc aatattgatt atatgagtgg
    20041 gctgatacaa attgcttata ttgacgctaa tgacaaaacg cctatcttgg atattaaacc
    20101 atacacgccg agtttggata gagtggaaaa tccgcaagtt ccagattggt gtagtcattg
    20161 gcctcacagc ttagaagaat ctgcttcttt tgcatgggaa aacgaattta atttttaagg
    20221 agtgatgaac catgagtttg caggattaca tttttgaaaa accagtaatt gaaacagaac
    20281 atcttatttt aagaacaatg aataaaaatg acatagatga tttgaaagaa tggattagtg
    20341 atatttcttt atacgagtat tgggggaaac gtccttcaaa aaacgaaaaa aatccagaat
    20401 tactttttaa taagccagag aaagctacta aaagttttca ttggggaatt gtccataaac
    20461 aagatagcaa agtgattggc gaactgtgga tataccttat tgaaaatgat agaatggcaa
    20521 aagtggcttt cagactttct aagcaatatc aaggtaatgg actaatgagt gaagtgttga
    20581 aaggcgtaat tgaattttgt ttttcaaaaa cagaattaca aaaaatatgg acagatgtgc
    20641 atattttaaa tcatgcttct tataaaacat tagaaaaagt gggttttcaa agggaaggac
    20701 atattcgtga ggggaaaatg gtaaatacct attgcgacta ttatctatat gggattttga
    20761 aatctgacta tatcattagt tgatgttatg gctaatattg aagcaacgat tgacgaagca
    20821 atggacaata cagttccaaa ggtctatgat acgaaaagaa ttaaaagata tgtgatgaat
    20881 ttattagata tggctcatta aaaagtacca aaggaataaa agttaccata tagacaaaat
    20941 tggtttttta tatcttttga tttcttataa gataatggca ttgtgcgaca agctatacaa
    21001 aaaattttca tgtatacttt gattataaat tatacaagga ggtactgata tgtacgcata
    21061 tgaaaattac aattatggcg ggggttatgt gggctatgtg gcaggaaaag gaaagaaaca
    21121 tacttttaac cttgcagaaa acttcgcaag ggataaatcg agcggtcaga aagtaaccta
    21181 tacgaaccct atcgctgtgg caaaaaacgg gggggctggc gttatggcta tggaaatatg
    21241 ttctatgtgg agcttgtgaa ccagtacctt gtcgtcaatc aagtatcggg cgaactggca
    21301 caaaagataa tgaatgaagc gttgaagtat caaggttgga aatacgtgta tgggggcagt
    21361 aacccgaaca cttcttttga ttgtagcgga ctgacccagt ggtgttatgg caaggctggt
    21421 atctctttac cgagaaccgc acaaatgcag tatgacgcaa cacagcatat cccactatcg
    21481 caggcaaagg ctggcgattt ggtctttttc cacagtacct acaatgcagg aagctatgta
    21541 acccatgtcg gtatctatgt aggaaataat cagatgtatc atgcaggcga ccctatcgga
    21601 tataccgacc ttaataattc gtattggcaa cagcacttga ttggtgctgg aagaataaaa
    21661 cagtagaaag gattggaaac catgtttaaa aagaaagaaa aaccagtaaa agaaaaaaag
    21721 tgcgtactgt caaggtaggc acacataaga aatctgtgat tgcattatgg gtagtgctta
    21781 tcgcaagtgt gagctttgga gtttataaga actttaccgc tattgatatg cacaccgtac
    21841 atgaaacaga aacgatacag cttagattaa atgacaccaa cgggattgaa aatttcgtga
    21901 agaacttctg taagtcctat tacacatggg acaacagcaa ggaagctatc gaagcaaggg
    21961 cgcaggcaat cagtaactat ctgacaaagg aattgcagga cttgaacctt gataccataa
    22021 gaacagatat accgaccagc tccattgtta caaatgtctt gatttgggat atagaacagt
    22081 cgggaacgga cgattttatc gctacctacg aagtagatca gcagataaaa gagggagaac
    22141 agacaacaaa tgtcaaagca acctataccg tcaaagtata tgtagataaa gacggcgata
    22201 tggtaatcgt acagaacccg acccttgctc ccgctatgga aaaatcggac tatgagccaa
    22261 aggcacagga agcggacaac agcgtggacg ctgataccac ggcagacgct acggcgtttt
    22321 tggaaacatt cttcaagctg taccctacgg ctacggaaac ggagattgcc tactatgtgg
    22381 tgggaaatgc ccttgaaccg ataaacgggg actatctcta ttccgagctt gtcaatcctg
    22441 tatttacggc tgacgaggat aacattaagg taaaggttgc tgtgaagttt atcgacaatc
    22501 agacaaaggc aacgcagatt tcacaatatg agctgacgct tcataaggac gggaactgga
    22561 aaatcatagg ttaatcaata aggcgtgtat atctgcaaag gggtacacgc ttccttaaat
    22621 ttgacaaact acactcctta tgttatactt gttttgtaag tatgaaaagt ataacaaaaa
    22681 taaaaagtta gaattgaggt gtaattatgc caatgaaaaa gggacaacac atatttggaa
    22741 cggttaaagt aggagaaaaa ggacaaatcg ttattccaaa agaagcacga caaatttttg
    22801 atattaaagc aggagataca ttattagtgc tgggcgacga agaacaggga atagctattg
    22861 tgaaagcaga tgttatgaaa gaattagctg tgaaaatatt gaaagggctg ggtaaattaa
    22921 gcaacggtat agatgatgaa aaataacatt atgcttatac ttccgatagc tttcatttta
    22981 gatttactta ttccttttat cttagctccc acatatagag gatatagtca tttaacacag
    23041 gttatgagtg ttcttggaaa tagtaaagca ccgttgcata atgtatataa tacatggctg
    23101 gtaatctttg gaatgacttt agcagtaatc aattttaagg tatatgcggt tgtttcaaag
    23161 gggtcaaggc taattgcaat aatccttttt atagtattgc tgatttatgc aatcggcggc
    23221 tgtattcttt ctggcttatt ctctgttgga gaagtaaaaa gtcttaatac tctttcagaa
    23281 aaaatacatg gttacggctc tgttgttgga tttatgtgtt tgtcatttgc accgcttctg
    23341 ttaggaattt atgcgtataa aataaatgac ataaaatttt catttttctg cattgggtgt
    23401 tttatattag caatattgtg tttttcatgt tttgttatgg gagataaacc taattttagg
    23461 aatacgtttt tagcatttga gggtttatgg caaagattat ctttattatt tatgtatttc
    23521 cctttagggt gtctgatatt gtttgaaaca tgacagtttt caatgtatac catatcaaaa
    23581 gataaataaa ttcagttatt tagccgtagc taaaaaatag gattggagtg ataccatgat
    23641 aagactaaaa agcgggatat taaaaaatgc tgaaacaagg ggcaaaattg agtggtgttc
    23701 cactatcgga aattcaagaa aacatagaag ccaccattga tgaagctatg aatagcaccg
    23761 acccagaagt acaggcaaat ttcaaaaaat attttggcaa caaaagaccc acgccagagg
    23821 aatatattta tacaatgaca aagaaatcaa gatttaaagt atgagagcct ttatttacta
    23881 cctacgaagt agatcagcag ataaaagagg gcgaacagac aacgaatgtc aaagcaacct
    23941 ataccgttaa aatccatgta gataaagagg cgatatggtt atcgttcaga accctaccct
    24001 tgcaccagcg attgaaaaat cagactatga gccaaaggca caagaagccg acgcaagcgt
    24061 ggacgcagat accgtcaatg acgctaccgc atttttggaa actttcttta atctgtaccc
    24121 gacagcaaca gaaaaagagc ttgcttatta tgtaacggga aatgtgcttg aacctatcgg
    24181 cagggactac ctttattctg aactggtaaa cccagtattt acaaaggacg gggacaatgt
    24241 gaaagtcaaa gtagccgtga aattccttga taatcagaca aaggcaactt tagtttcgca
    24301 gtatgagctt gtactatata aggatagtaa ctggaagatt attatgtaag acattttgtt
    24361 ggcaggctct attttcggat atagtctgcc ttttctgtag caaatgtact gcactgacaa
    24421 gctatttcat tgtattttaa gcaatggaat ctttcttgac aattttacct taaatcatat
    24481 atttatagct attgactttc tgcttgcaat aagctataat atgactataa aattaaatca
    24541 gtcatatagg aggcatggtg tatgtataaa gaagttggca tttcttcaaa taatgttccg
    24601 ataacacttt ctatttggta tacagaaaga agtagtccta ccattgtttt tttgccaggt
    24661 actatgtcac accctctcat gtatgagaat tttctatgtg gattgagcga aagagggttt
    24721 aatataatag gtgttcacta tctttcacac ggaaaaagcc ctaaaatcaa aaaaaattat
    24781 ataatggaag atatgttgca taatgtatat gacgcaacaa cttacggaat agaaactttc
    24841 ggtgaaaaca tagctgtcat gggttccagt caaggcggga ttttagctgc aatggcggca
    24901 ggtagggaca caaggttaaa agccgttttt ccacataata tcatgctgac tacattaaaa
    24961 gaaactatga gcttaacaaa atatcccgca tggtcgcacc gttttatggg gctgatacag
    25021 tgggggtttc gtgtaggtgg aaaattgctg cctaactaca aggttccagg agacgcttat
    25081 ttagactatg acagagtatt ttatagtgaa caggcaaaac aagattgctt aaatgaccct
    25141 atgcttttac cctattatcc tttaagattt gtcgccagct tatttaatgc tgacttatcg
    25201 tgcctggaga atggggctat acaatgtcct gttattttaa ttactgccaa aggcgacccg
    25261 ctttttagct ttgattatac caacaaagta tttgatttaa ttcatgtacc gcaaaaggaa
    25321 ttacttgctt tagagcttaa tcgtcacatg atttttaatg aggacacgga cactacaata
    25381 aacgcattgg aagctacctt gcataaatat cttggatagc atattgatta tataatcagt
    25441 aaatgttata cttttcatga cgatataaat attttagtca taaggaggtt atgagtttgc
    25501 cacgatttac ggaacaagaa aaagaaatca taaacagtaa acttttgata gagggagaaa
    25561 agctgtttgc attacacggc ttaaaaaaag taacagttga tgatttagta gctgctgtaa
    25621 acatatcaaa aggttctttc tatgcttttt atcccagtaa agaacatcta tatgttgaaa
    25681 tcaattttcg cttacaaaaa gagttattta caagtataga aacaaccatt aaaaagaaaa
    25741 agtataagaa ccatagagat ttagcaaagg acgttattat gctaagttta acgggggtga
    25801 ttacttcgcc tattctttcg cagattgatt tatctttaat ggactatctg caaagaaaaa
    25861 tatcgtcaga tatttttgaa aatcatatgt acagtgatat tcgtatattg gagatactgg
    25921 aagatttagg ggttatattt ttggttccgc atacagttat cataaaatcc ctgtattctg
    25981 ttttatcctg tttggaacaa ttcaaagagg acgaagaact gaatatgata caaaatcttt
    26041 tagtgaatgg tattattcag caagtagtag cagagtaaca attttgtttg gttatttgac
    26101 aggcaggtag ccaaacaaat tttttacagt ttatatgact atatatctat aaacgtcata
    26161 aagttaaagg aggaatgaat aatgattgat gtaaaaaaac tgtattttag ctatactgac
    26221 aaaccgtttg tggagaatgt aagtttccat gttgggagag gagaaatttt tggtttttta
    26281 ggaccctctg gtgctggaaa atcaactatt caaaaagttt taactgggct taatacaaga
    26341 tataaaggca gcgtaaaagt tgcaggtaca gaaataaggg aacatacaaa tcagttttat
    26401 gaaaatattg gggtagactt tgaattttca acctgctacg agaaattcac agcacgacaa
    26461 aatctcgcct attttgcttc tctatatgaa aagcaacctc ggtctattga tgaattattg
    26521 catatggtag gcttggaaaa tgacggggac aaaaaagttg ctgacttttc caaaggtatg
    26581 cgttctcgct tgaatttcat taaagcgtta gtccatgacc ctgatatatt attccttgat
    26641 gaacctacaa gcggtcttga ccctacaaat agtcgtttaa tgaaagatat tattcttgcg
    26701 gaaaagaaac gcggaaaaac gattatcatt actactcaca atatgtttga cgcaacagag
    26761 ctatgtgacc aagtagcctt tatcgttgcc ggaaaagtca gtgcattgga tagcccgcat
    26821 aatttgatta tgtcaagagg agctgccaaa atacaatata cctactatga taaaggcgag
    26881 aaaaccggag aatgtctgct tgatagaacc acagaagata agctgttaaa aagtttaatt
    26941 tccgaaaacc gtttgctttc tattcatagt agtgagccta cattaaatga tatttttgtt
    27001 gatattacag ggaggacact tcaatgagat tgggacgttt aattcgtggt gatatacatt
    27061 tccaatggaa atatggtttc tattttatct actttatatt aacagtttta tatgtgtgcg
    27121 ggattgccgc tttgccggaa cattggaaaa cggatattgc ttctattatg atctactctg
    27181 acccggcagc aatgggattg tttttcatgg gagcgattgt tcttttggaa aaaagtcaaa
    27241 aggtattaaa tgctatggtg gtatcccctg taaaaatatc ggaatatata ctttcaaaaa
    27301 cagttgcgtt gattgctatt tctacggtaa tcgcattgat attaggggta gtttccggca
    27361 gcaatcattt acttggtatc acagttggta cggctttgac ctcagcaatc tttactatgc
    27421 ttggaattat tgcagctaca aaaatttcaa acttgaacca atttctcatt gtgattatgc
    27481 cgatagaaat tgtttgcttt gcaccaccta ttgttggtct gtttgtgaag ttaccatatc
    27541 tgttccgctt ttttccattc acagcttgca tgaatcttat aactggaaaa agcgttttac
    27601 tatcgtttga tatggtactt gtaattgcta cgttgattat cctctacata gttgcgcgac
    27661 atacagttaa gcatatgtgg aaaagtttag gaggtgtaaa gctatgaaaa ggatattatc
    27721 cagtaccata caggtattta gacaaatcaa gagcgaccct atgatgtttg cagcttgttt
    27781 tacgcctttt gtcatgggag ctttgattaa attaggtatt ccctttcttg aaagaataac
    27841 agatttttct ttgcgagcat actatccgat ttttgattta ctactttcta tcatggctcc
    27901 tgtattactc tgctttgcat ttgcaatgat tacattagag gaaatcgacg ataaagtgtc
    27961 acggtacttt tcgattaccc ctcttggtaa gtcggggtat ctctttacaa ggttgggagt
    28021 accctcaatt atttcggcgg tcatcgcttt tattgtactg ttactttttt cattagaaaa
    28081 gttgtccatt ggaatgacaa tatgtttggc gcttctcggc tcagtacagg caattattgt
    28141 ttcacttatg attattacat tatcaagcaa taaattagag ggtatggcag ttacaaaact
    28201 agctgcactt acattgctgg gaataccagc cccctttttt attgatagtt actaccagtt
    28261 cgcggttggc ttccttccat cattttgggt agcgaaagcc atgcagaatg aggcggttct
    28321 ttatttctct ataggattgg tggtagcttt agtctggtac tacttcctta caaaacgtct
    28381 gtttcggaag ctggcaggat aaggaggcat attatggaaa agggtagact tctgcaaaac
    28441 ggaaaagtaa atagagctat tcaggtttcc aaaatcacac tatttatcaa cctaagttta
    28501 tcgttactta aattcgctgc cggatatata ggaaagtcaa gtgccatgct gtcggacgct
    28561 gtacactcgg cttctgatgt attaagcaca atcgttgtga tggttggaat taaaatatca
    28621 gaaaaacagc ctgatagaga acatccttat gggcatgaac gtatggaatg tgtggcttcc
    28681 attattctct ctgttgcatt ggctataacg ggtgctggaa taggatattc aggtatcaag
    28741 aaaatctttt caggacagta caatacgctt tctgtcccta gtggaatcgc acttacggcg
    28801 gcagtcttat ccattgttat aaaagagtgg atgtactggt ttacaagaaa tgctgcaaag
    28861 catacaaatt ctgacgcact tatggctgac gcatggcacc accgctctga tgcactctcg
    28921 tcggttggtt ccttaattgg cattttagga gcgcgtttag gctatgctat tctcgaccct
    28981 attgcaagcg ttgtgatttg cggttgtatc ttaaaagctg ctttggacat tttcaaagaa
    29041 agcattaaca aaatggtaga ccatagttgt gacaatgcta ctgaaacaaa gattagggaa
    29101 gttgtgcttc agcagcaagg ggttgatggt attgatgaat taaaaacccg catgtttgga
    29161 gcgaaaatgt atgtagatat tgaaatatta gctgatggta atttagctct ttatgacgct
    29221 catagaattg ctgagggagt ccatcaaacg attgaaaaca actttccaca atgtaagcat
    29281 tgtatggtac atgttaatac gaatgaactt ctctattctg cataggacag ggaagatgat
    29341 ttaaggctat tgaaagggaa aggagggaag taggttatgg aacaatactg gtggattatc
    29401 attgttgtgg ttttgattgt cgttggctta cttaaaattg taattatgaa gaagtataac
    29461 caaaggagaa attctaacaa tcgttcaaaa gatagcgagg acgacaagat gtattaaggt
    29521 ttattgaaat ctgttatgtt gtagaatgat gtttttagat actagaaagt gctttaaggg
    29581 ggggctaacg ttaaggaaag ctttgagtac aattctttac aatcaacagt cttttgcata
    29641 gagaaatgta tgtaggaaat atggtgcaag gcataaagca ccagcgatcg agagatcaac
    29701 ctatgaacca aaggcacact acattttata ctacttgaca agtaaacgag cgtttactat
    29761 aatggtttac agaagtaaac gttcgtttac taaagaagga ggttgtaaat gtctgacaca
    29821 aaagaaaata tattaaaaat tgcactacac ttatttgcag tgaatggtta tgaggctgtt
    29881 tctgttagta tgattgcgga agccttggga atgacaaaag gagcattata taaacattat
    29941 aaaaataagc aagatatttt tgactgtatc gtagaacgta tgtatcaaat tgataataag
    30001 cgagctaagg attatcaagt accagaaagc acaatagagg taacccctaa tgcatacaga
    30061 aatacaacta ttggaagtct aataacattt actcttgcac aatacgattt ttgggtgact
    30121 gatgaatttg cgtctaattt tcgcaaaatg ttaatactag agcaatatag aaatttaaaa
    30181 atgaatgtgc tttatcaaaa atgtcttgta aatggtccta tcagttatat agaagatata
    30241 tttaatgaaa tgattaaaaa aggtgttctt caagagtgtg atacaaaaca gcttgctatt
    30301 gaatattatg cgccattatt tctattgata agtgtagcag acgcagttga aaattgttgg
    30361 gatttaaaat ctcaattaac cacacatgtt aatcaatttg tcaagaaata tattacaatt
    30421 gaaaaagtaa aataatcata ttctgtcctg aatgcaaaca taacttttta atttcagcag
    30481 aggaactcaa aatgaatatt gttataagtc acaaatatta actattaaaa tattacaata
    30541 atggagatga gaaaatggaa tatattcgca gtaaaaaata ttttacaccc gacttacaga
    30601 caaaaattat gggaccgaat cctataaaat tacaagagga actattacaa aatcataaaa
    30661 tcccgtctgg cagctatgtc tgtgaccttg gaagtggaca aggtttaact tctgtatttc
    30721 ttgcaaaaga atatggattt gttgtttttg cagttgactt atggagtgag ccaaaagaaa
    30781 atgaagaatt tttcaaaaaa atgggattat cttctgcaca aattaaatct gtaaaagctg
    30841 acgccaatga acttaaattt gagaaagagt tttttgatgc tgtagtaagt acagattcat
    30901 ataattattt tggacgtgac cctatatatt tagatgaaaa attgcttcca tttgttaaga
    30961 aaggtgggtt tgtttatatc acagttcctg gcatgaagaa agattgccat gagaaacttc
    31021 cagaagagtt attgttatca tggacaccag aagaacttga ttatatacat gatataacat
    31081 attggtctaa cataattagt cattgtaaaa aggcagattt aatttcaata tatgaaatgg
    31141 aaagtaattc ggaagtttgg tcggattgga taaagcaaaa taatgaatat gcaataaatg
    31201 accgtaaggc aatagaagct ggcggtggag attatctgaa ttttattgct ataattctac
    31261 aaaaaaagta aattctttag aaccaaacac tataatttgc gttctaatat gtttaccgat
    31321 tgacatcaat aattttattt ttgagggtaa tgatttgaaa atagaagaat aattccagtt
    31381 tgtaaaagtc aataaatcca aagataaatt tgtatttgaa aggagttttg aaaaatgaaa
    31441 aaatatgagt atgtagaaat taactataat gcgaaaggag ttgtattcct ttgtactgat
    31501 aaacacaaag aaattattga cagttacgca aaaacggggt atcactacgc agggtttgta
    31561 cccacagaaa ttgacgcaaa aggctgtatg cgaaagatag atttaatttt tgaaaaagaa
    31621 gattaactta ttttagggag ttggttctaa tgataggact taaaagacga aatatgaatt
    31681 atattatgaa agttggtgct aaagctcaag gtgtttctgt atctgaattt agggctatgc
    31741 tacaagatac catagacaaa actatggaaa gtacagaccc agacgtgcag gcaaatttta
    31801 aacgatactt tggaaataag cgcccaacac cagaggaata tatttatacg attaccaaaa
    31861 agacaaaggt taaggtttaa atgttgtaaa gggggcttgc cgtatgaaac gctttatgta
    31921 caattcagta tattcaagcc caatttaaca gcatactatt tacaattaaa tacgctatac
    31981 cagtagctcg tacaaaattt atattttgtg cgggcttttt taatacccga aaagtttttt
    32041 taattatttt catttttttg gaagcaaatc acaccttgct gtacagatat ggtgcggaaa
    32101 gagggataaa cacttttcaa ttcatagctg gaaaggaggt gagattatga aaccgtctga
    32161 cttccaaaaa acaattcagt gtcagtttga atgtaagctg aaaaaagttg tcaaaggtat
    32221 tatctggaac taccgtaaag aattaaaacg aagaaaaaac aaggaaatat cttttagcga
    32281 attaccagac attacggtag aaagtctggc aacatgggac gactacgaaa caaactacac
    32341 aatttttaat gtgtgtggca tagatattaa agtccttgat gatgaacttg cagaagctct
    32401 caaacagcta cccgaaaaaa aacgtaacaa tgtattgatg tattacttct tggaaatgag
    32461 tgatacagag attgcagata ttatgaatta taccagaggg gctgtgttca aacacagaca
    32521 ccgtgcccta gaaactatga aagagatact aaaggagaag aaatagaaat gaagaaaaag
    32581 tacaagcacc cgtcttatta tttgatttca tcagctatgg acggggatga aactgcgatt
    32641 gagaaattac tggtattcta tgacccgtac atatcaaagt gttgtttacg tcctttatat
    32701 gatgaatacg gcaatgttta tattgttgtt gatatggaat taaagggacg tattcgagaa
    32761 gccttaatca aaatgatttt agactttgag attgatatag atatagaaac cgaagaagaa
    32821 taagcagata ccagcagggc gtgattggtt aaatccccct ctttcctgcc ctgctcctgc
    32881 ttttatacat ggaagcctac ggctttcgcc gattgttctt tgacaactga ataaagcaga
    32941 cagatacgtt tgtgatacag cgagccacgg ggactgtacg ccatgacctt tccgacaaga
    33001 aagtgagcga cccacgcagt gatccgagag caacttgtgg aatggtcgct ttgccatgac
    33061 ctgctatcac agaataatga tacgtccgca tggtgcggtt ctgcccacaa gaatgggaat
    33121 agttgagata ctaacggagc tttccgaagc cgtctgtctg cttatgataa gacatacaga
    33181 aaggagtgtt gcaacatgaa caatactgat gtgcctatct gggaaaaata tacccttagt
    33241 atagaagaag cgtcccgata ttttcgtatc ggggaaaaga agttgcgtag gcttgccgag
    33301 gaaaatctgg actctggctg ggttatcgtc aatggaaacc gtattcagat aaaacgaaaa
    33361 cagtttgaaa aaattattga tacactggac gaaatctgat gtcttagagc cgtagatatg
    33421 gtataatatt agtagcgtat cacggctcat tccatgacgg aaaggagctt tacaccatgt
    33481 caaaagtgaa aagacaagac agtaaaggac gcaatttgcg tcttggagag agccagagaa
    33541 aagacggaag atacgtttat aaatataccg atatattcgg gaagccacag tttgtatatg
    33601 cttggaaact tgtaccgaca gacaaaacac ctgctggaaa acgtgatgat atatcgttga
    33661 gggaaaaaga aaagcagata aaaaaagacc ttgacgacgg tatcgacaca gtgggtggta
    33721 aaatgacagt ctgccagctt tacgaaaaga agaacaatca aagaaagaac atcaagagaa
    33781 acacaaaaat aggacgacag tatcttatga acgcactgga aaatgaccca ttgggtatga
    33841 gggcgattga tacggtcaag cagtccgacg ctaaagaatg ggctatcaga atgaatgaca
    33901 aaggttattc ctacaaaacg atagataact ataagcgttc cttaaaggct tcattctata
    33961 tggcgataca agacgactgt atcagaaaaa atcctttcaa tttcaagttg agtgatgttc
    34021 tggaagatga tacggaagca aaggttatcc taacaccaga gcaggaagaa aaactccttg
    34081 cctttatgga aacagataat gtctacagca agtatcgtga tgaagttatt cttctgctgg
    34141 aaacgggact tcgtatctct gaattatgcg gactgacaac acacattgat atgcttaacc
    34201 gtgtgattaa catagaccac cagctattgc gtgatacaga ggtgggttac tatatttcca
    34261 cacctaagac aaaaaacggg aagcgtgagc ttccattgac agaaagagca tatcaggcac
    34321 ttgaacgaat tttaaagaac cgaggaaaag cacaaccgct tgttgttgac ggttacagca
    34381 acttcttgtt cttgaaccgt gagggattgc caaaggtggc aggaaattat gaaagtatgg
    34441 tgcgtgggct gattaagaag tacaacaaga cgcataagga caagttgcca aacatcacgc
    34501 cccattcatt cagacatact tactgtacga acatggcaaa taagggaatg aaccctaaca
    34561 ccctgcaata catcatgggg cacgcaaaca taaccatgac attagggtat tatgctcacg
    34621 gtactttcca gtctgcaaag gctgaattag aaaggctggc ttcttaatat cgaaaatgta
    34681 tcttactact tatttactac ttttggttgc attttcatgc tggtaaatgc catcttatgc
    34741 gaggtatctt ccaaaaggaa aatgccaata aagcccgaaa atatgggata tatcggcatt
    34801 taccaactta tgaaaagata atcagaaatt tagtaagttt tttaaaacaa aaaataaaaa
    34861 caaaggaaga cgcaataaag ttcttagcaa gaaagctata tgatgaaggc aagttaacat
    34921 cttatgagga atatttagaa gctgtaatgt acagagaaac tttatctcca acaggttttg
    34981 aaagagggct tgcaattcct catggaaaat ctaagtcagt taaagaagct gcatttgcag
    35041 ctatgacctt agagacacca atagaaaatt gggaaagcgt agacccaaat aataaagtag
    35101 agctaatatt tttaatagca gtaccagaaa gtgaaggtgg aaatacacac atacaaatac
    35161 tttctgagtt agttactaga ctgtcaaatg aagattatat gaatatgttg ttaaattcaa
    35221 aaactaagaa agagttattt aataacttag atataaaaga aaagaaagaa caaataaaag
    35281 aagagattag gattcaaaat gaatcaaaaa aaattatatt agcaataaca gcatgccctg
    35341 ccggtatagc acatacatat atgtcagcag aagcattaat taaagctggt aaagaaatag
    35401 gtgtggatgt gtatgtagaa aagcaaggag ctaatggtat ggtggaccct catacgccag
    35461 atataataaa aagggcagat gcaataatat atgcaacaga tgtagcacct aaaaatacag
    35521 aaagatttga acatttgcca aacataaaaa cgtcagtagc agctccacta aaaagagcaa
    35581 aagaaataat atacgaagct ttagaagtag cacaaaaaca aggtaaaggt gattatatag
    35641 aaaaatcatc tgatatatca tattgtgaaa aatcatcatg gaaaaaagat gtaaaatcag
    35701 caatacttac aggagtatct catataatac cacttattgt ggcaggaggt atgatacttg
    35761 catttgcagt attagcatct caagcatttg gtctacaaga actatacaat caagaaggtt
    35821 cttggttatg gcaattaaga aagctttctg gaggtatgtt aggtacattg atggttccag
    35881 tattatcagc atatatgtct tactcactag ctgaaaaacc aggtcttgga ccaggatttg
    35941 cagcaggtct agcagctgat ttaataggta gtggatttct tggaggtatg ttaggaggtt
    36001 tacttgctgg ttatatagta aaatttttaa agaagaatat accagccaaa ggcacattag
    36061 ctggattcgt aagtttttgg gtatatccag tagttggttc tttggtagta ggtttattga
    36121 tgttatttgt agtaggaaag cctgtagcag cacttaacaa tggtcttata aattggttaa
    36181 atgggatgac tggagtaaat gcaatactac taggtgcaat aataggagct atggtttcat
    36241 ttgacttagg tggaccaata aataaagcag cctatacatt tgcaatagga gcaatagcaa
    36301 gtggaaattt tataccttat gcaatatttg catcagttaa gatggtttca gcttttggag
    36361 ttacaatagc tactgtttta ggcagagatt tattttcaga atcagaaaag gaaataggaa
    36421 agcaaacttg gatattagga ttggctggaa taactgaagg tgcaatacca tttatgatag
    36481 aggacccaat ccgtgttata ccatcattgg tagctggttc agccataact gggtctatag
    36541 tagcattctt caatataggt cttcaagtgc ctggagcggg gatattctca ttggcgcttt
    36601 tagagggaag tatgtcagga tttattatag catcaatatg gttctttgcg gcagtacttg
    36661 gagctataat atcagcaata ttattaataa taactaggaa acaaaaaaat caaagaaaga
    36721 tacattaaaa gatacgttat tagatattta aatcaaaggt ttaattatag tgataaaata
    36781 aatattaaaa aattttgtaa actcagtagt atttttattg gatttttaat tagacagaaa
    36841 atattatcag ataagttttg gaggataagc aatgaaaaaa aatgtacaca tagttcctca
    36901 tatgcattgg gatagagaat ggtacttctc aactgaggaa tctagaatat tacttgtaaa
    36961 taatatggaa gaaataatgg atatgttaga aaataatcat aactatccat attatgtgat
    37021 ggatggtcaa actgctatat tagaagatta tttagcagtt aagcctgaat gtaaggagag
    37081 aataaaaaaa ttagttcaag aaggaaggct aataataggt ccttggtata ctcaaactga
    37141 tgaaatggta gttggtggag aatccatact tagaaattta ctatatggaa taaaagattg
    37201 tgatgaattt ggagaatata tgaaaatagg atatttacca gattcatttg gtcaaagtgc
    37261 tcaaatgcca caaatactaa atgggtttga tataaaatac tcaatgtttt ggagaggatg
    37321 ttctgaaaga aaaggaacta ataaaacaga gtttaattgg aagagtgatg atgggtcttc
    37381 tgttttggtt caaatacttc cattaggtta tgcaatagga aaatatctac caatgaatga
    37441 agatgagcta agaacaagaa tggataaata tttaccggtt cttgataagg gtgcaactac
    37501 tgaccatata atacttccaa atggacatga ccaaatgcca attcaaaaga atatatttga
    37561 agttatatat aaattaaaag aatgttatcc agacagaaaa tttttcttaa gtagatatga
    37621 aaacatattt aaagagctag aaaaaaatga ggatatagat acaataaaag gggaattttt
    37681 agatgggaaa tatatgaggg ttcatagaag tatattctca acaagaatgg atataaaatc
    37741 agcaaatgct agaatagaat caaagataac taacatattg gagccactag cttctattgc
    37801 atattctcta ggatttgaat atcatcatgg attaatagag ttaatttgga aagaaataat
    37861 gaaaaatcat gctcatgaca gcattggatg ttgttgttca gataaagttc accaggaaat
    37921 aatgaataga tttttcttag ctgaggaaaa agtagaccaa ttaataacat tttacaaaag
    37981 aagaatagtt gatgcaatga gttgtgaaat ggctttagat aagttaatag catttaactt
    38041 aatgccttac gataggaatg aaatagtgag cgcccaagtt ataactaaaa tgaaagcatt
    38101 tgaaatattt gataaagata ataataagtt ggattttgaa gtggtacata aagaagtaat
    38161 agatgcagga ttaatagacc gtcaaattgt tcattatgga aactatgatt catttatggt
    38221 atatacaata aactttaaag ataaaatacc tgcaatgggg tataaggcgt atatgataaa
    38281 agaagttgaa catatgttag aaaaagctta tgaagtatgt gacatggtag ataatgactt
    38341 ttatactatt caagttaatg aaaacgggac attaaagata tttgataaaa aattaaataa
    38401 aacttttgaa aatgtattat tgatggaaaa tggaggagat gaaggagatg agtatgattt
    38461 ttcaccactt ccaaatgaga aattaatatt taacgataat atagtagcaa attccactat
    38521 aaagaaaaat agatttaata atgagataaa gataaattat agattaaaag taccaaaaga
    38581 tatacaagct agaaaaaata atagagcaga ttcatttatt gactttgata tagttataaa
    38641 tgttccaaat gataaagcta taatagatgt agtattcaac ataaataacc aagcaagaga
    38701 ttgtagggtt agaacttata taccaacaaa tatagcttca aagttttctg tgtctgataa
    38761 tcagtttgga catataaaga gaagtgtgta tgatgatgct atggaagttt ggcaacagga
    38821 agactggagt gaaagaccag attctatata tccaatgcta acattcgttg gattgtctga
    38881 caaagagcat ggagtagctg ttcttacaaa cagtacaaga gagtttgaaa tagtagggga
    38941 aaaatttgac acaatagcta taacattatt tagaagtgta ggattcctag gtaaagaaga
    39001 aatggcaaga cgcccaggtc gtccatcagg aataaagttg cctactccag attctcaaat
    39061 gataggagat ataaaaatag acttagcaat agttactcat gaaaaatcta cactagaagc
    39121 aaatgttgca aatatggcta aagagtattt aacaaaaata caaacttata ataaaatgcc
    39181 atataatgct atgaaattga atcaatcaga aatagaagta gattattcat atagtttact
    39241 taaggaaact aataaggacc ttgtgttaag tgttgtaaaa aaggctgaaa aagaagaggc
    39301 attagtagtt agattttata attctaaaga agaaacaaaa tcagctaaca tagtatttaa
    39361 caaaaatatt aaaacagcta aatggattaa cctaaatgaa aaagaattag aatctatatc
    39421 aatagataat aactctgtaa atgtagagtg cttaatgaac caagttaaaa ctatactggt
    39481 taaatagtgc taaaaatgat tagatagtgc taaaaaggaa ttatcttaaa atagagataa
    39541 tcctccttat cattgttgaa ataataaaca tatatttgat tttatacaaa agagtgtctc
    39601 atgaactaag aaatttattt tgagacactc cctttttata agataaaaga atattaatgg
    39661 tataagttgt atacaaagag agatgtagag tataaaataa aagataaatt aagcttataa
    39721 aattatatgt ttaaaataag gagtaataaa aaggagtaga taagtatgaa aatgttaatt
    39781 tctatagatt ctttgaaagg aagcttatct tcaatagagg caggaaatgc aataaaaaag
    39841 gggatattga aggttaaaga agatgcgcaa gtaaagatat tgccacttgc tgatggtggt
    39901 gaagggacag tagatgcttt agttcaaggg atgaatggta aaaaagagac tatagaagtt
    39961 actggaccaa tagctaaaaa agtggatgca acatatggat tattaaaaaa tacaagtaca
    40021 gcaataatag aaatagctca agcatcagga ttaacattgg ttccgactga acttagaaat
    40081 cctttataca ctacaactta tggtgttggg gaaataataa aggaagcaat aaataaaggc
    40141 tatagaaatt ttatagttgg gataggtgga agtgctacta atgatgctgg gataggtatg
    40201 cttcaagcct tagggtttga gttttatgat aaaaataaca agttagtagg attaggtgga
    40261 aaagttttaa atgaaataag acatataaaa atagaaaata gattaaagga attagatgaa
    40321 tgtaagttta aaatagcctg tgatgtaaat aatccgctgt ttgggaaaaa tggagctgca
    40381 tacatatacg ggtctcaaaa aggtgcaaca tcagaaataa ttgaggagtt agataatggg
    40441 cttagaaatt tttccaaagt agttaaaaat tacttaagta aagatgtagc taatgtagaa
    40501 ggagcaggag cagcaggagg attaggtttt gcttttttag catttttaga ttcaaaatta
    40561 gaatctggaa taaaaataat attagaagaa attaaattag aggaagagtt aaaagatgca
    40621 gattttgtca taactggtga aggtagattg gacaatcaaa ctgctatggg caaggctcca
    40681 ataggagttg caaaactggc aaaaaaatat ggggtaaaag taataggttt ggctggagcc
    40741 acaacagaag atgcagttaa atgtaatgaa gagggtatag atgcttattt ttctatagta
    40801 aatagagcta tgacaataga agaagcaatg gataaagcaa ctgctagtga gaatatgaca
    40861 gctacaacaa cacaaatatt caatcttata actagtatac aacagtcaaa aaatatgtag
    40921 tattaaaaca aggtatatta ggtaaaaagc tgtcacatat taatttagac tttttagtgt
    40981 gacagcttat ctattttatg ttaataactt aagaaacata tattggaaat aaattaatgg
    41041 atttagatat tttgaataag ataagctatt ctattcataa aactactata gttatctgcc
    41101 ttagtaagtt ctttaacatt attccattca gataatattt caataaagct ttcaaatatg
    41161 taattgaatt tttctttttc tttttcattt ataggtatca tagctataat ttgaactttt
    41221 tcctcacccc atttaactgg tttatcgtta acaattaaac atactccggt ttttaaagca
    41281 tccattttca tgctatgagg aattgctaca ttgttattaa atgcagttga agacattttt
    41341 tctcttataa gaacatcatc tatataattg ggtttgacat atttattttc gtacagaata
    41401 tttcccataa atcttatata atctttagca ctatctaaat atatattttt cataaacaaa
    41461 gattcactaa agtacatttc taaatgattt tgtacagtaa gtatattttt tctatcttta
    41521 atctgaataa atttactttg aatattatca taatcctttc tatttaggta aggatttaca
    41581 taaacaaaag gtatagggca tctatcgtta attggtaaag tagtaataac catgtctacc
    41641 ttattgtcaa aatcaagatt atgagtatct gaaaaaatct gaataatttc tatggattca
    41701 ttaaatctct tctcaaggtc tcttactatt ttgaattgta aatcatagta atttggagta
    41761 attaatgcac atagaacttt atcttctaat aatttttgtc tatcaaagaa tgaacctaca
    41821 tgaagagata tataagatat ttcatcttcg tctatatcta cattttctaa agtttgaatc
    41881 tgattgcaaa tataaacaga tatatcatat atcaaagggt aggaatcttt taacttttga
    41941 ggtatttgat ttcttaaaac ttgattattt ttggctctac tgattaaatt ttttacatga
    42001 agagtaaatt ttacaacaaa ttcctcatca gataaatcta taagatataa gtcatataca
    42061 tttttaatta tttttttagt taatttaaca aagtgttcat ctatataatt tgacaatgat
    42121 ttagtatcca tttgattgta gtttaagact gtagttttgt ttgttaactg aaatactaaa
    42181 tagtataatt ccgcactagt taaagttata ttgtattcag attctaaaaa atttgatatg
    42241 tcttttgcaa tatttatatc tacgttattg tctatgtagt ttactacttc aacagagtct
    42301 atgtaattat tcttttttat tctgtctata gttatcatta aatgaagaag tatgttgttt
    42361 atagcatact catttataaa taaattgtat ttttttaata tatatattat attttctttt
    42421 agtttagtca aattatactc ttgatatatt ttttgtacat caaataagct taaaaaatca
    42481 cttcccgttt ctttaaatat tatagagctc attaacttgc gctttgcaga ctcatttcca
    42541 actagtttca ataaaaattt cgattgcttt atctttaaat tataacttcc tataaattta
    42601 ttagctttga ctatatcccc ttcaatagta gctgggctaa caaataattc ttcacttaaa
    42661 tcaaatatat taattccatt actattaact ataagttctt ttaaaattaa atttaatctg
    42721 ttatcaggtg atatattctc tgaactatca atcttagttt gacaattagt attgagattt
    42781 actttatagc cctttttaga agataatata aatgcaccat tagaattaat ctcgtttaca
    42841 tattttctaa tcgaacgtgt tgatacatta aggtaattag ctaatgtgga tgaatctatc
    42901 caatcatgtt gcgtttttat gaaatttaaa aatagctctt ttttagatgt tttcattaga
    42961 ttccctcgca tagttttaaa ataaaatctg tatgatatga taattatata attgaatgta
    43021 cgtattataa gatacattat actatattaa taggtctaat taagagtaaa tgatttccta
    43081 ttattaggaa atatatttat gtgaaatgaa cttatttggg aagtaaaata taaacataat
    43141 aaaataaaaa gttcattaag aaataaatta atggataaaa atattggagg tattattatg
    43201 aaaaatataa gagtgctttt ggcgtgtggt tcaggagcaa gtacaggttt tatggcaact
    43261 agtatgagaa agagtgcaaa gaaaaaaggt atagaagcag atataaaagc gagaagtgag
    43321 tcagagatag ataagtattt atcagaaata gattgtttat tactaggacc acatttagat
    43381 tatatgaagg aagaaattca acaaaaggct aatgaatata acgttccagt agcatgtata
    43441 tctaaaactg catatggaag actagatggt gcaaaggctt tggaagaagc actagaacta
    43501 atatcaaaca aataaaatat ttatggggag agtgtttatg aataaattta tagatttttt
    43561 aagtgaaaaa tttactcctg ttgtaaataa tatgactaag aatatatggg tacaatcagt
    43621 tcaatcaact ataatgaaag ttttgccaat ggtatttgta ggctcattag taactatagt
    43681 ttctgtcttg aagaattaca taagcttttt acctgattta agtccaataa atcaatatac
    43741 atttggactt ttaggattat ttatagcatt tttattgcct atggaaacaa tgaagaataa
    43801 gaaatttgaa agtatgagtg tagtagctgg tcttgcaggt gctggattaa tgttaatgat
    43861 gattcgtcca gagattacga atgaaggagc tatatttaac ttcaacaggt ttggaggaga
    43921 aggaatgctt gtatcgctag ttgcaggatt attctctgga ttaataatgt ctttatttgg
    43981 ttctttttca tttttcggag aagattcagc acttccggat tttgtaagca agtggtttga
    44041 taatatgtta cctataacag tgataatgtt tgtaggctgg ggattaattt atggactcaa
    44101 ctttgacatg tttgaaataa tagttagagt atttaaacca gtatcagata tagcacagac
    44161 attacctgga tttgtactct taaacttctt agtagtattt atatattctt taggaataag
    44221 tgggtgggta ctatctccta taacttattc aattcaactt ggtgcaattg cagctaatgc
    44281 agcagcagta gcatcaggag gagtagcaac taatatatat gtttacgaag ttattcaaac
    44341 aggctggata actttaggag ggttgggtgc tacattacca ttagttataa tgatgtgttt
    44401 atccagagtt ggaagaataa aagcaatagg aagagcatgt attgtccctt ctataatgaa
    44461 tataaatgaa ccagtaatat ttggagctcc agtggcatgg aacccatttt taatgttacc
    44521 tatgtggata ggaacagtag taatatcaat aactacttat ttatcaatga gtatgggatt
    44581 tgttacaatt ccaagtaagg tgtttaattt atggtatgtt ccttttggta tatctacttt
    44641 tatggtaaat caagatgtta gaggtcttat tgttttagca ataaatatag tattgctatt
    44701 tatgatatgg tatccatttt ttaaagctta tgagaaagaa gagataaaat tagagagtca
    44761 agaggtttaa atttagaaat tgaaaggaaa ttataaatat gacaattgaa gaacatataa
    44821 tagatgaaga attagtagaa gtagcaatgc aaataatact tcgtgcaggt gaggctagaa
    44881 cagaaattaa acatgctctt aatgatttgg aaaagtttga ttatagaaat gctgatttaa
    44941 aactagctaa agcaaaaggg tttatgacag aagcacatag agctcaaacc aagattatac
    45001 aaggggaagc tggtggagag aaaagagcac attcgctgtt atttgcacat gctcaagata
    45061 ctcttatgac catattcagc gagttaaata taactatgtc tttagtagga atagtaaagt
    45121 ctatagaata taaggtaaat aatagtaaat gatttaaata aataaaataa ggagtgatta
    45181 aattgggtaa gtttcctaaa ggttttttat ggggaggagc tgtagcagct aatcaattag
    45241 aaggtgcatg gaatgaaggt ggcaaaggtt ggtgtttagc tgatgtacat atgtatgata
    45301 aagatagaga tatatctaaa ccttatgagt ctgatatgac tatagaaaga ataaagtttg
    45361 ctatgaaaga taataatgga tattatccta agcgtagtgg aatagacttt tatcatacat
    45421 acaaagaaga tttaaagctt ttgaaagagc tagggttaaa gtgttttaga ttttcaataa
    45481 attgggctag aatatttcca aatggagatg atttagagcc taatgaagag ggattaaagt
    45541 tttatgataa tcttataaat gaagtaatag ataatggtat ggaacctatt gtaacactac
    45601 ttcactatga gactcctttg aatttagtat taaaatataa aggatggtct aatagaaaag
    45661 tggtagattt ctttgttaga tatgcagaat taatactaaa tagatataaa aataaagtta
    45721 aatattggat agttataaat caaataaatt taatacatta tgaatcattt aattcagttg
    45781 cattttgtgt agaccaagtt gataatgtag aagagactag atatcaagct attcatcatc
    45841 aattcatagc tacagctttg actaagaagc ttggaaaaga aataaattca gatatgatga
    45901 taggtatgat gatggcagat tgtacatctt atccagaaga ttgtgaccct gaaaatgtag
    45961 tatttgcttt aaaaagaaat aggctacaat attttttcac tgatgtaagt tttaggggag
    46021 agtatccagt ctatattaag agatttttca aagaaaataa cataaatata gttatggagg
    46081 aaggtgatga taaaatctta aaagaaaata ctatggattt tctagcttta tcatactatt
    46141 attctacaac agtatcagca agtaaaaaca ccatgaatcc aactgacaat ataaagaatc
    46201 catatttaaa agcaaatcct tgggggtggg caattgaccc aaaaggatta tacaatactt
    46261 tatctcaata ttatgataga taccaaaagc caataatgat agctgaaaat ggatttggaa
    46321 tgcatgataa atttgaagat ggaaaagttc atgatagtta cagaatagat tatttaggaa
    46381 aacatataga acaaataggt gaagctatag ctgatggaac cgaaatatta gcttattgta
    46441 tgtggtcgcc aatagatata gtaagttgtt catctcaaga aatggaaaaa agatatggat
    46501 ttatatatgt ggatatagat aacaatggaa atggaagtaa agaacgttat aaaaaagata
    46561 gtttttattg gtatcaaaat gtaataagaa ctaatggaga agaattaaga gaaaagtaaa
    46621 tagtttttaa atataaacaa ccccccttat aaatactgta tatattatta atatagtttt
    46681 ataagggggg ttataattgt aaataaaatg atttattaat tatatgattc atagcgaaat
    46741 ttatatttat actt
//
LOCUS       DAGSWI010000013        46355 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.013, whole genome shotgun sequence.
ACCESSION   DAGSWI010000013 DAGSWI010000000
VERSION     DAGSWI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.013"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            389..3937
                     /locus_tag="MJF80_000694"
     CDS             389..3937
                     /locus_tag="MJF80_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBZ0279369.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINNEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLKDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYENGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSADIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADMALY
                     SAKGKGKNCYTLYDKKIDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPITHVGDLCYKAIWNNQDKLCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            4122..6056
                     /locus_tag="MJF80_000695"
     CDS             4122..6056
                     /locus_tag="MJF80_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBZ0279370.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMEDKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGINKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6162..7157
                     /locus_tag="MJF80_000696"
     CDS             6162..7157
                     /locus_tag="MJF80_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBZ0279371.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7129..7914
                     /locus_tag="MJF80_000697"
     CDS             7129..7914
                     /locus_tag="MJF80_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBZ0279372.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7902..8600
                     /locus_tag="MJF80_000698"
     CDS             7902..8600
                     /locus_tag="MJF80_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279373.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLKASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8723..9196
                     /gene="ybaK"
                     /locus_tag="MJF80_000699"
     CDS             8723..9196
                     /gene="ybaK"
                     /locus_tag="MJF80_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBZ0279374.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9393..10232
                     /locus_tag="MJF80_000700"
     CDS             9393..10232
                     /locus_tag="MJF80_000700"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBZ0279375.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10245..10793
                     /locus_tag="MJF80_000701"
     CDS             10245..10793
                     /locus_tag="MJF80_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBZ0279376.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10859..12040
                     /locus_tag="MJF80_000702"
     CDS             10859..12040
                     /locus_tag="MJF80_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBZ0279377.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQREMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12335..12721
                     /locus_tag="MJF80_000703"
     CDS             12335..12721
                     /locus_tag="MJF80_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0279378.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYNMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12871..13134
                     /locus_tag="MJF80_000704"
     CDS             12871..13134
                     /locus_tag="MJF80_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBZ0279379.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13263..14105)
                     /locus_tag="MJF80_000705"
     CDS             complement(13263..14105)
                     /locus_tag="MJF80_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBZ0279380.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14400..15134
                     /locus_tag="MJF80_000706"
     CDS             14400..15134
                     /locus_tag="MJF80_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0279381.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            15144..16277
                     /gene="nagA"
                     /locus_tag="MJF80_000707"
     CDS             15144..16277
                     /gene="nagA"
                     /locus_tag="MJF80_000707"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBZ0279382.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16376..17125
                     /gene="nagB"
                     /locus_tag="MJF80_000708"
     CDS             16376..17125
                     /gene="nagB"
                     /locus_tag="MJF80_000708"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBZ0279383.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            17300..17572
                     /locus_tag="MJF80_000709"
     CDS             17300..17572
                     /locus_tag="MJF80_000709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279384.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            18232..18936
                     /locus_tag="MJF80_000710"
     CDS             18232..18936
                     /locus_tag="MJF80_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0279385.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            18938..20425
                     /locus_tag="MJF80_000711"
     CDS             18938..20425
                     /locus_tag="MJF80_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0279386.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIEFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            20630..22243
                     /locus_tag="MJF80_000712"
     CDS             20630..22243
                     /locus_tag="MJF80_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBZ0279387.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(22605..23435)
                     /locus_tag="MJF80_000713"
     CDS             complement(22605..23435)
                     /locus_tag="MJF80_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0279388.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKTYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            23508..25235
                     /locus_tag="MJF80_000714"
     CDS             23508..25235
                     /locus_tag="MJF80_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279389.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSVSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            25272..27134
                     /locus_tag="MJF80_000715"
     CDS             25272..27134
                     /locus_tag="MJF80_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279390.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            27467..28156
                     /locus_tag="MJF80_000716"
     CDS             27467..28156
                     /locus_tag="MJF80_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBZ0279391.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            28261..28947
                     /locus_tag="MJF80_000717"
     CDS             28261..28947
                     /locus_tag="MJF80_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBZ0279392.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            28964..30871
                     /locus_tag="MJF80_000718"
     CDS             28964..30871
                     /locus_tag="MJF80_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBZ0279393.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            31324..32013
                     /locus_tag="MJF80_000719"
     CDS             31324..32013
                     /locus_tag="MJF80_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279394.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            32449..32988
                     /locus_tag="MJF80_000720"
     CDS             32449..32988
                     /locus_tag="MJF80_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0279395.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            33007..34050
                     /locus_tag="MJF80_000721"
     CDS             33007..34050
                     /locus_tag="MJF80_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279396.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            34052..34888
                     /locus_tag="MJF80_000722"
     CDS             34052..34888
                     /locus_tag="MJF80_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0279397.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            34993..35667
                     /locus_tag="MJF80_000723"
     CDS             34993..35667
                     /locus_tag="MJF80_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0279398.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            35667..36719
                     /locus_tag="MJF80_000724"
     CDS             35667..36719
                     /locus_tag="MJF80_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBZ0279399.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            36949..37797
                     /locus_tag="MJF80_000725"
     CDS             36949..37797
                     /locus_tag="MJF80_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBZ0279400.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            37787..39451
                     /locus_tag="MJF80_000726"
     CDS             37787..39451
                     /locus_tag="MJF80_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBZ0279401.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLNKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKILIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            39500..40756
                     /locus_tag="MJF80_000727"
     CDS             39500..40756
                     /locus_tag="MJF80_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBZ0279402.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            40779..42860
                     /locus_tag="MJF80_000728"
     CDS             40779..42860
                     /locus_tag="MJF80_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBZ0279403.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKPKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            42897..43982
                     /locus_tag="MJF80_000729"
     CDS             42897..43982
                     /locus_tag="MJF80_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBZ0279404.1"
                     /translation="MRKYMNEFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            44036..45160
                     /gene="wecB"
                     /locus_tag="MJF80_000730"
     CDS             44036..45160
                     /gene="wecB"
                     /locus_tag="MJF80_000730"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBZ0279405.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENINSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKHSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            45238..46212
                     /locus_tag="MJF80_000731"
     CDS             45238..46212
                     /locus_tag="MJF80_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBZ0279406.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT        18502 a         4687 c         8021 g        15145 t
ORIGIN      
        1 tttaaatttt atgtatacaa aattaatttg ctaactgaat catataaaaa aaacaaaata
       61 ttttttagag ggaatgatta gatagaattt ttttgttatg aaattaattg aagagtaatt
      121 aattagaaaa ggaatgaata ataatgaata tattacatta ttactttttg ataaatcaaa
      181 aataaagtta taatttttaa tattaattaa gaatgatgaa atatgaataa agattttaca
      241 aataaatctt aagctatctt atttaattct aatacaggaa gaaggtaaat gtatattgaa
      301 aaaatcatag aaatagaagt aaacaattta aataaataca aaggtttgca aaagaagtaa
      361 tataattata tacaatgagg atgtgagtat gatgaacaga ctaaagccaa gaattttagg
      421 tattagtatc ttgtttatta ttataattat tttaacatgt ggagctgttt tcaagtttaa
      481 atttaaatta gataataaat taaaagaaac cacacataca tatttgtctg agtttacaga
      541 acacaatata attaatctca agacaagact tagtggtcaa tttgacatgc ttgaatctat
      601 tgctaacttc ataggaaact tgaataatat aaataatgaa atggtgattg atttaatgga
      661 ttctgatgta aagcgaagtt ctttaattag aatgactatc tctacattag atggggaatg
      721 ttattctaat gatggaatca cccataatgt tcaagataga gattatttta aaaaagctaa
      781 aaatggagaa aagaatattt cagaaccttt aaaatctatt gttaataata aagaaataat
      841 agttttatct accccagttt ataaaagtgg gaaaatagtt ggtgttttaa gtggaattta
      901 tgattcaaaa aagttaaatg agttattagg attatctgca tttgatgaaa aagcagatgt
      961 ttttgttgca aagaaaaatg gagatatagt tgcccaaaaa acaaataaaa atagaaagac
     1021 gctatcatgt atatttcagc tttttgacaa agataattgt gatgaagatg attgtctaaa
     1081 aactataaaa gaaaatatga aaaatgataa gtctggatat tttaattata acttagataa
     1141 tgaaaaattt atgttaaact atcagccact tggagttaat gattggtatg ttttttctgt
     1201 tgtgccaaat aaaatagtat ctattcagtc agagaatata tcttatgatg tatatatttt
     1261 tgcattagaa atactactag ttatattgat ttttataata tatatagcat gtattataaa
     1321 taataacact aaaattatta taaatgaaaa agaaaaattt aaagtactca ctgataatat
     1381 actaggaggg gttaaaacct gtttaaaaga tgaatatatg actatgacat atgtaagtca
     1441 ggggttttta gagcttacag gttactcttg tgaagattta aataaaatat ttaaaaatag
     1501 atttaaagat atgatttata aagatgattt agacaagttt aattgtatta ttctaaatca
     1561 attagaaaaa caagataaaa ttgaagttga atatagaatt gtaagaaaag atggaagtat
     1621 aatttggatt ttggagagaa gtaagtctat aaatgataat aaaggaagtg agtgtattca
     1681 gggagtttta actgatataa cagaacttaa gaaaatacaa gaagaattag attcaaaaag
     1741 atttgaaata caggctatca atgatagtat tgttggtgga atagtgataa ctgagataaa
     1801 tgatgatttt gatagtatat atttaaatga aggttattta aatatgattg gatatacacg
     1861 caaacaactg aaggatgagt taaataataa attaatcaaa cttattttta aagatgatag
     1921 aacagaatta attaatagta taaacaaaca actcaaatta agtgatacat ttgcatctga
     1981 acaaagagtt gaaaaaaggg atggcactat tatatggatt ttattaaaag gaagaaagat
     2041 gatttatgag aatggaaaat ctgtactttg ttgtgttatt gtagatatta cttctgctaa
     2101 attattagaa caagaactta aaaagagtga agaacgtcat gaaattattc taaagcagac
     2161 gcaaaatatt ataatagaat gggatataga aaaagataaa atgaatatat cacctaattg
     2221 gtataataag tttaaatatg accctataac tgaaaacgca acaaaaaata taattaaatc
     2281 agcagatata tatcatgatg atatggataa gtttatttca ttaattaaac ttacttcaca
     2341 agggagtgaa tatgaagagt gtgaaattag acttagaact agagatacta tttacgcttg
     2401 gtttagtata aaggcaacaa ctttatttga taataatggt aaaccttata aagtagtagg
     2461 aagtttgact gatattgatg aatcaaaacg agaagctgaa aagttgaaag aaagggcaga
     2521 aaaagaccct cttacagatt tgtataataa aaaagcatca caatcactta tagaagagta
     2581 cataacattg gataaaaaaa gaaaaggtgc tttaatgatt attgatattg atgactttaa
     2641 aggaataaat gataatttag gacatttata tggggatgct gtattaagtg aaattgcaaa
     2701 tgacctaata aatctattta gagcaaccga tatagtagga agaattggtg gagatgagtt
     2761 tattgtattt ataaaagata tttctgaaac aagagatatt ataaataaag cagaagaatt
     2821 ggtaaaaaca tttgaaaggt cttttttagg taaaggacga aattataaaa tatcattaag
     2881 tgtaggaatt gctaagtttc cacaagatgg tatggactat atgacactat ttagaaatgc
     2941 ggatatggct ctttactcag ccaaagggaa gggtaagaat tgttatacat tgtatgataa
     3001 aaaaattgat aaagtccaat atataactaa tacaagaaat gcaggagata gtgataatgt
     3061 agagatgtct tttaaagaac atatcacaaa ttatatattt gatatactat atgaatcaag
     3121 tgatatagaa gtggcagtaa atttaattct aagtattgta ggtaaacact ttaatgtgag
     3181 tcgcatgtat atatttgaaa attcagatga taatcgagta tgtaaaaata catttgaatg
     3241 gtgtaacgaa ggtgtaacct cggaaattaa taatcttaaa aatatttgtt acaaggattt
     3301 aggcaactat cttttaaatt ttaatgcaga tgggttattt tattgtaatg atattgaaaa
     3361 tttaggtagt gatgtgaaaa aagtacttaa gccacaaggc atacgttcaa tattacaatg
     3421 taaaatttta gagggtaatg aatttagagg gttcatagga tttgatgaat gttcatataa
     3481 tcgtatgtgg actaatgatg aagtgaacac tttgacattt atatctaaaa cgctcagtat
     3541 gtttttatta aaaatgcgta ttcagagtaa gctagaagag tctttaacaa ttcaaaaatc
     3601 tgtgatggat aatatggata tatgggcata tgttgttgac aggaatacct atgaattact
     3661 atttatcaat aaaaaaatgc ttgagtcagc accaatcact catgtaggtg atttatgtta
     3721 taaagcgata tggaataatc aagataaact ttgtaaaaaa tgccctatga tatatttaaa
     3781 agatggtgat gatacttata aatcattcat gcataataaa aagtttgagg tacaggcaaa
     3841 tataactaca actactttga aatggtttga tggtaaagat gcatgtttaa tatttggaag
     3901 tgaaataact agtattgaag ctgaagaaaa tacttaaaat actgtgttat atatatactt
     3961 aaaatcgcaa tttgaattta ttagacttaa aatgtctata taaatcattt aagttgtggt
     4021 tttaagtatt ttttatgata taattaagcg ttaagaacaa aattaaaaga tacttttgta
     4081 taaaaaaata tttgttaaat atatattgag caggtgataa tatggaaaat aagtttttac
     4141 caataagcaa acaagatatg atagatagag gatgggaaga attagatttt gttctagtta
     4201 caggagatgc ttatgtggac catcatagtt ttggaacagc aataatatct agagttttag
     4261 aaagtgctgg gtataaagtt ggaataatag ctcaaccaga ctggagaact acagatgatt
     4321 ttatgaagct aggaaaacct agacttgcat ttctagttaa tgcaggaaat atggattcaa
     4381 tggtcaatca ttattctgta agtaaaaagc atagagaaaa ggatatgtat tctccaggtg
     4441 ggaaaatggg ttatagacca gatagagcta ctatagtata ttgcaataaa ataagagaag
     4501 catacagtga tgttgcaata gtaataggtg gaattgaagc gagcttaaga agatttgcac
     4561 actatgatta ttggagtgat aaggttagaa agtctatgtt aatagatagt ggagcagatt
     4621 tattagttta tggtatgagt gaaaagcaga ttgtagaagt agccaatgca cttaatgatg
     4681 gatatgaccc aaaatacatt agacatattg atgggacatg ctatatttca gatacattgg
     4741 aagaaatata tgataaatat atattaattc catcatataa agaaatttgt gaagataaaa
     4801 tgaagtatgt agaagctttt aaaattcaat atgatgagca agacccattt agaggtaata
     4861 taattgttca acctcatgga agtaagtatc tggttcaaaa taagccagaa aaaccactta
     4921 gtagagaaga actagatgaa gtatatggat taccatatca gaagacatac catcctgtgt
     4981 atgaaaaatt tggaggaata ccagctatag aagaagtaaa atttagtata gtaagttcaa
     5041 gaggctgttt tggtagttgt tcattttgtg caataacatt tcatcaaggt agagcagtgc
     5101 aaagtagaag tgaaaaatca attattgatg aagcaatagg aataacaaat ttagatgatt
     5161 ttaaagggta tatacatgat gttggaggtc caacagctaa ttttagaaga ccagcatgta
     5221 aaaaacaaat tactaaaggt gcttgtaaaa atagacaatg cttatctcca tcaccatgta
     5281 aaaatttaga tgcagaccac agtgaatact tacatttact tagagctgta agaaaattac
     5341 ctaaaataaa aaaggtgttt gtgcgttctg gaattagata cgattatgta atggaagata
     5401 agaacaataa atttcttaga gagttgatag agcatcatgt aagtggtcag ttaaaggtag
     5461 ccccagaaca tatatcagaa gaagttttaa agtatatgca aaaaccagca ggaaaaactt
     5521 atgataaatt tagacagaag ttttttgcta taaatgaaga attaggtaaa aagcaatatt
     5581 tgatacccta tttaatgtct agtcaccctg gttctacctt aaatagcgct atagagttag
     5641 cagagtattt aagagatact cactaccaac cagagcaggt tcaggatttt tatccaactc
     5701 cagggactct atctacaacc atgttttata caggtattga cccgcttaca atgaagccag
     5761 tttatgttcc taaatcaaaa cgtgacaagg caatgcaaag ggctttatta caatatagag
     5821 cacctagaaa ttatgatttg gtatactctg cgttagttga agcaggaaga gaagacttaa
     5881 ttggttttgg acatagatgt cttataaagc caaaaaatga aaagccttat tttaatagaa
     5941 ataattctaa aaaaaatgta tctaaaggaa taaataaaaa caaaaaaact aatacaaata
     6001 acagaagtaa taaaaatcaa caaaaaagtt caactaaaaa aaagaaaaaa cattaaagta
     6061 taaataaaaa tacttaaaaa agaaggatta ttcgataaat taacagaaat tagtataaat
     6121 aaatataatt attttaatga cgttaccttt gggaggaaga gatgaaaaag tatatttcta
     6181 taatattatt agtggtgttg actatggttg ttttggtagg atgttcacca ggaaaagata
     6241 accctaaaaa taaggagcta tcagttgtca aacaagtaaa agtagctgtt ccagatggac
     6301 taccagcagt ggcaatagct aaacttgcaa atgaaaatcc agagattaaa gaaggatatg
     6361 aaacagtgta tagcattgaa aagacacctg aggcgatttc cactagagtg atgaaaaaag
     6421 atgcagatat agctatagtt ccttcaaaca tggctgcaat agcgtataat aaaacttcat
     6481 catataatat tgttggaact gtaggaatgg gttcacttta tttggtgtca acagaaaata
     6541 taaaagatta ctctgattta gttggtaaag aagttggatg tactggaaaa ggacttactc
     6601 cagacataac tataaaaagt cttttgttac aaaaggatat aaattattca gatataaaat
     6661 ttaattatgt aaattcagca agtgaactgg taccattatt ggctactggt aaagttaaaa
     6721 caggtattgt tccagaacct gcacttagtg ctttgatgtc taaaaatcca gacataaaaa
     6781 ttataaaaag tttaaatgac tcatggaagg aagtaagtgg ttcaaaagat ggttatcctc
     6841 aatctacttt aattgtaaag tctgaatttt taagagataa taaagatttt gttgattctt
     6901 ttgtaggtca attatcaaat agtatagatt gggcaaacaa aaatcctgaa gaattaggtg
     6961 catattctga aaaaataaaa atatctactg aatcaaagat aataggaaaa tctcttgaaa
     7021 gagctaattt aaaatatatt ccagttaagg acatgattaa agattataaa aattactatg
     7081 agaaattagc taattttgat gataagacat taggaggcaa agtaccagat gaagcaatat
     7141 actttgttga gaaataaaaa aaataaagaa caatttatag ataaaatgga ggtaacactt
     7201 tcttgtgtta tcctcttatt tttatggcaa attattgctt taaaaattga taatgatatt
     7261 tttttaccaa cagtaggtca agtatttaat agtataaaag agattatgct aaattctagt
     7321 ttctatatag atatacttta ctctatgggg agatgttttt ttagtttttt actggctatg
     7381 atttttgcta ttatactttc aatagtgtct tatttaaata gattttttag aaacttatta
     7441 aagccaatca atgctttgac acgttcaata ccaactatga tattggtagt acttgcactt
     7501 atttggtttg aaaaggatag tacccctttt atagttggat ttactatagt ttttcctata
     7561 ttgtatgata acgttttggg cgctatattg aatatagaca agaacttatt agaaatggct
     7621 aatgtgtata aaataaggtt tatagataag gtgttaaaaa tttatttacc agccataaaa
     7681 tttcaaatta tatctatact agtatcaaca ttctctttaa gtttgaaagt tgctatagca
     7741 ggagaggtat atagtcaacc tacatatggt attggaacta tgatacagac tgaaaaaatt
     7801 aactttaaca catctggaat atttgcatgg ataattattg ttcttttaat atctgctatt
     7861 ttacaaatag ctcaaaagtt tttagcaagg agagcttttt tatggaaaag ataaagttga
     7921 gtattgaaaa tataaataaa agatatgata gtcgtattat atttagagac tttaatattg
     7981 atttttatgt aaatgaggta aattgtatcc ttggaaaatc tggttgtgga aaaactacac
     8041 tcttgaacat aataagtggg attattaaaa atgacaccaa caatctaaat atcaaagaaa
     8101 atttaaatcg agtaggaaat aagcttaaag ctagttacat atttcaagat gatagactta
     8161 tagactggtt aacagtagaa gaaaatataa agatagttgt aaataaatac tataataaaa
     8221 ctcaattaaa taaaatttgt gatgaatact tggaattggt tggaattagt gattataaaa
     8281 aattctatcc tcagatgctt agtggaggta ttagacaaag agtaaatatt gctagagctt
     8341 ttatatatcc atcaaaaaac attataatgg atgaaccttt taaatctatt gatgcaaaga
     8401 atacacagct tataatggat aatttcagaa atatattaag aaaagaaaag agaactgtgt
     8461 tatttgtaac tcataatatt gaggaagcat tgtttttagc agataggata tttattttag
     8521 gagactctcc aattagaata aaaaaaatat taaaaaattc taaggaattg gaaaaaaatg
     8581 aagttttgaa gttgatatag agttataata atactgtcgt tttaaaatat atttggttaa
     8641 aatctataag actatattaa aatagtacag aatattcaaa actgtgtata atgttcgtat
     8701 tttataaaaa ggaagtgaat gtatggttaa gactaatgct atgagaatat tagattcaaa
     8761 taagattgat tataaagtaa tgtcttatga agtgaaaagt gaacatgtgg atggtgttga
     8821 ggtggcacat gatataggaa gagatgtaaa tgaagtgtat aagacattag taactcaagg
     8881 agttagtaag aatatatatg tatatgtaat accagtacat gaaaacttag acttaaaaaa
     8941 ggctgcaaaa gtagctaaag agaaaagtgt agagatgatt catgtaaaag atataaataa
     9001 gcttacaggt tatattagag gcggatgttc tcctataggt atgaaaaagc tttataaaac
     9061 ttttgtaaat gaaagtgcaa agaatttaga tacaattatt gtgagtgcag gaaaaatagg
     9121 atatcaaata gaactatctc catttgattt acaaagactt ataaaagtag aatttgtaga
     9181 tgtaataaaa aaataagaat tacatataaa atatagagct tatattctta ataataggtg
     9241 ttgtagtatt aataaaatga ataataattt taaaataaat attcagttac ttatgtgtaa
     9301 ttggtataaa aatattgaga aattttaaaa acaatggtaa agtgtaagta taatcacgaa
     9361 tattttttat aaagtaatgg agggatgttc gcttgagaaa aataaaatct gaacaaatcg
     9421 tcgagcaggt aaaaaaactt tgtatagaag cgagcctata tttaggagaa gatgttttaa
     9481 gttgtataaa agaaaaagcg aaaagcgaaa agagtgaagt tggaaaaaat atattgaata
     9541 tattagttga aaatgctgaa atagctaaag aaaaaaatat accaatatgc caagatactg
     9601 gtatggctgt attttttgtt gaaataggac aagaagtttt aattgaagga gacacactta
     9661 ctgatgctat aaatgaggga gtaagacaag ggtatgaaga aggctactta agaaaatctg
     9721 ttgtaagtcc aattaacaga gtaaatacaa aagataatac acctgctgtt attcattatg
     9781 atatggtaaa aggtgataaa attaaaatag aatttgcagc taagggattt ggaagcgaaa
     9841 atatgagtaa gatgaaaatg ttaaagccat cagatggatt ggaaggaatt aaaaaattca
     9901 taatagatac agtttcagaa gctggaccta acccatgtcc tcctatggta attggagttg
     9961 gtataggtgg gactgtggac aaatgtgctc aaatagctaa aaaggcactt tttagagaac
    10021 taggtgaatt taataaggac gaaaatatag caaaacttga aagtgaacta ttaacagcta
    10081 ttaataaact tggtatagga cctcaaggct taggtggtac tacaacagct ttgggattaa
    10141 acatagagac ttttcctaca cacatagcag gtctacctgt tgttgtaaat ataaactgcc
    10201 atgcatcaag acataagaag gtagtaattt aggaggaaaa taaaatgata aaaataacta
    10261 cacctgtaaa tgaaattgat atagcaaaat taaattgtgg agatacaatt agtttgagtg
    10321 gtatacttta tacagcaaga gatgctgctc ataaaagatt gatagattgt ataaataaag
    10381 gtgaagaatt accatttgat gtatatggtc aaggaatata ttatgtagga cctactccta
    10441 caaagccagg agaagttata ggtgctgcag gtccaacgac gagttataga atggatgatt
    10501 tgactatacc tcttttagaa agaggtctta gattgatgat aggcaaggga aaaagaagtg
    10561 atgaagttat agaaggtatg caaaaatacg gagcagttta tcttgctgca ataggaggag
    10621 ctggagcgta tatttcaaat tcaataaaat catgtgaaat tattgcatat gaagatttag
    10681 gagctgaagc tataagaaag attgtagtag aagaccttaa acttacagta gcaatagata
    10741 gctatggaaa taatatatat gagcaaggtc gagcaattta tgaatgtaaa taatgtttag
    10801 aaataaatat gaatatcaaa attaaaaata gttgtgtata ttttaaggag gaaaattgat
    10861 gtctaaggat tatgcaaaat tggcattaga aatgcatgaa atcaacaaag gtaaagtaag
    10921 tgtagaaagc aaggtagaaa ttaaaactaa ggatgattta tcaacagcat atacaccagg
    10981 ggttgctgaa ccttgtttaa aaatacatga aaatcaagat gatgtttata gatacacttc
    11041 taaagggaat ctagtagcag tagttagtga tggaagtgct gtacttggat taggtaacat
    11101 tggtgctgaa gcatctatac ctgttatgga aggtaaagct atattattta aacaatttgc
    11161 agatgtagac gcttttccaa tatgtttaaa gactaatgat gtagatgaga tagttaaatc
    11221 tgtagagttg atggagcctg tatttggagg tataaatttg gaagacatat cttctccaag
    11281 atgttttgag atagaagaga gattaaaaaa atctttaagt atacctgttt ttcatgatga
    11341 ccaacatggg acagcaataa tagttgcagc agccataata aactcaataa agttaattga
    11401 aaataaaaaa atagaagatt tagaaatagt tataaacgga gcaggagctg ctggtattgc
    11461 catagcaaag atactactta atatgaatgt taaaaatata attttatgtg atagaagtgg
    11521 agcattggaa gctagtatag aaaatctaaa ctatgttcaa agagaaatgt taaaagtaac
    11581 aaatataaga aatgaaaaag gacctttaaa agatattata aaaggaaaag atgtatttat
    11641 tggcgtttca ggtccaggag ctgttacaaa ggaaatggtt gaaagtatgt ctgagaagcc
    11701 aataatattg gcaatggcaa atcctactcc agagataatg ccagaagaag caaaattagg
    11761 tggagcattt ataatgggaa caggtcgttc agacttccct aatcaagtca ataatgtact
    11821 ggcttttcct ggaatattta gaggtgcact tgatgttaag gctaaagaga taaatgaaga
    11881 aatgaagata gctgctgcat atgcaattgc taatacaata agtgatgaag aaatatgccc
    11941 agagtatata ttaccagatg tatttaataa gaatgtagta aaaaatgttg ctaaggcagt
    12001 taaagaagca gcgataaaaa ccaaagtaaa tagaatataa atttttatat gttcatatat
    12061 ccttatctta tcgggtatat gggcattttt attataggtt tatactcata tatccttact
    12121 tttatataca tatctttatg attaaaggtg ttagaaaatg tagtaaattt tatactatca
    12181 gactctatct ttattacttt agtagataca tggtggtaaa ggtttataga aaaaaatata
    12241 ggaaggcaga tttttattgt tgcgtatata tgagaatgat ataatatgaa tattataaat
    12301 taataaattt aaatttatgg aggtacaaga gatgatggaa tataaagtta atgaccaaga
    12361 acttaatgct tcaatgttta tcccattcgt caacaggata tggccaggag attataatat
    12421 ggataaaaca cagactgcat tatcaaaaac tttgaatata actgcttatg atgataaagt
    12481 gcttgtagga tgtctgcgag ttctttcaga tggttattat tttggaacaa ttacagaatt
    12541 gcttgttctt ccagagtatc agaaacaagg cattggtagt aagcttttcc agcttgctaa
    12601 agagaacacc ccaactatgc tatattttgg tgcacagcca ggagtagaag agttctatga
    12661 aaaaaatggg tgccaaagaa gtctgcaatc ctacataata aaaaagaaag atagttatta
    12721 aagttccagc tttcaataat cttaaaagta caatatttta gtggaatcat ttcatttttt
    12781 attactaata tgacataaac atgtcatatt agcattgata tacttttaat atggttaaga
    12841 gccaaagaaa aatataagga gtgatttaaa atggaagaaa ctaaattttg ccaatgctgt
    12901 gcaatgccaa tgggacaaac tgatgaatta tatgggacaa ataaagatgg aagtaaaagt
    12961 actgattatt gtagttattg ttttaaagat ggtgagttta cagctgatat aagtatggaa
    13021 gagatgatag aagtttgtat ccctcatatg cttcaaagta ataaggatat gactgaagat
    13081 gaagcaagaa caatgatgaa caatttcttt ccaacattaa aacgctggaa ataattttat
    13141 gcaaaattag ttatatgcaa aacatgaaat atggtgttga gaataagggg tggtgtgctt
    13201 tatagcatac cacctgtttt gttacttgga gttcagttat tttcctaaat attttaaata
    13261 tactatttta gataagttcc ctcttcatct tgataaatta atcctttttt cataagcact
    13321 ccaagagctc gtttaaaatt tttcttactt gaattaaata atatagaaat atctttaggg
    13381 tcagatttat cattaaatct catatatcca tcagaacctt ctaaataact taaaatctta
    13441 ttttctaaag tatcaagttc atcctttcta tttcctctag gagaaagacc aagttttcca
    13501 tcttcatata tttttatgac atgtaaattt tcaagaacat caccagtttt tagttcagta
    13561 aagtactcat tatgaagtat tacaccagca tatttattgt ctacacatat cattgcagaa
    13621 ttattagttt ggaatccata tacaactcca gttacaatat ctcctacatt gtaagtatgg
    13681 tctgttaata agtatgaatc tatgtctgta gtagcagcaa ttcttttgct tttgtctaaa
    13741 tatatataaa ataaatattt ttcgtctcta aatagtggat aagtcttagc tttaaaagga
    13801 actaatatgt ctttaggaag tcccatatct ataaaagtac caatgtctgt attatcaact
    13861 acttttaaat gtgctacatc cccaacctta gctaaaggcg gtattaaagt tgcagcagtt
    13921 ctgtcatcag aatctttaaa tatgaaagca tttacttcat cacctatgtt tatttcattt
    13981 tttccgatta atctattatg aagaagtata tcgtcttttg tgttattagt ttgtccatcc
    14041 aaaaaatatc caaattcagt ttttcttttt actgtaagtt tattaaaatc acctatgttt
    14101 atcaaattaa aatcatcctc tcattttata tttataattt agccataata tacttatgta
    14161 gtagtttgtc acagtatcta tattagtatt agtgaaattt acaataaata atatagttat
    14221 ttatgtattt ttcaattctc tagcttctta tatagtataa actccaattt cttttttgtc
    14281 tttaggtttt attaaaattt ttacaaaata agttaaaaag tattgaaata aaatttcttt
    14341 taaagtataa ttggtatata ccaaaattgg tatataccat atcgaaaagt ggtgactata
    14401 tgaaaatagt aaataaatct agtaatatac cattgcatac tcaattaagt tcaataataa
    14461 gagagatgat agaaactggt gagcttaaag agggcgatgc aataatgcca gaaagagaac
    14521 tttgtaacat acaaaatgta agtcgtatga cagtaaataa aaccattgtt ggattagtta
    14581 cagaaggact tctttatagg gtacaaggta aagggacatt cgttgcaaag caaaaaaaga
    14641 agtatcagtt ttcaaatgta aaaggtttta ctgatgttat gaaagaaaag ggagtaaaca
    14701 taaaaacgga tatattatcc tttgagatgg aactaccaga tgatttagtt aaaagaaagc
    14761 ttggaataag tgataatact acaaatatat ataagattgt cagacttaga tatactgatg
    14821 gagagccatt tggattagaa atagtatatc tatcagaaga aatgtgtaag ggtctaacaa
    14881 aagggatatt ggataattct tcattatata gggttttaaa tgaaaaatat ggctataaga
    14941 tacaaaaagc tgaacaggta atggagccag taatactttc agatgaagaa agtaagcttc
    15001 ttgaaacaga tgaaggtgct ttagctctta aacttcatag aaattcctat aatagagaag
    15061 gtagccctat agaatatacc atatcaatat ttagaacaga taaatatcaa tatgaaatag
    15121 tattgagtga gtaggtgaat aatatgaaat gtttaattaa tggaaagata atattaaaaa
    15181 atcaaatctt agaaaataaa gtattagtat ttgatgaaaa aataattgat atagctgata
    15241 gtgtaccaaa agattgtgaa gttatagatg ctgatggaaa atatatatct ccagggctta
    15301 ttgatattca tatacatgga aacatgggaa aagatacaat ggattcgact gatgaatcta
    15361 ttgaaacaat ttcaaaatca attatgagac atggagtaac atcttttctt ccaactacaa
    15421 tgactatgga taaagaacat gtttatgatg cacttgaagt tattaaaaaa gcccaaaata
    15481 gaaagcttga aggggcacaa gtattaggag cacatttaga aggacctttt ataaatgaga
    15541 attataaggg agcacaaaat gaaaaattta taataaattc aaaatacgaa tttataaaag
    15601 aatataaaga tgttataaaa gttataactt atgctccaga aaaagatatt gattttgatt
    15661 ttactagaga aataaaaaga tgtacagata tagttttatc tattggtcat agtaatgcaa
    15721 attatgacca agctaaagaa gctataaatt taggagtaac taatgtaact cacatgttca
    15781 atgcaatgac tggtttaaac catagagacc caggcgttgt tggtgcagca cttacaacta
    15841 atgtgtacag tgagttaata gcagatacaa tccatataaa taaagattta tttcaattta
    15901 tactaaataa taaagggaaa gaaagactga ttttaataac agattctata gaagctggtg
    15961 gtcttgaaga tggcaattat tctcttggag gtcagaaagt catagttaag ggtaatgaag
    16021 ctagacttga aaatggagca ttagctggaa gtgtgttgtc tttaaataaa atggtattta
    16081 acttcttaga taatacaaac cttaaagtga atgaagcaat taatttagct tctttaaatc
    16141 cagcaacatc tttgggaata aatgacaaaa aaggaagctt agaaataggt aaggatgcag
    16201 atatagctgt atttgatgag aatttagatt gtaaaatgac actttgtttg ggggacgtag
    16261 tatataaaaa tatatagtat acaaaacaaa ctttaaagac agtaaaaaca atataaaaaa
    16321 taataaatta aacttacatt agttaaagta gtagttaaaa tttaggagga ataagatgag
    16381 agtattagtg tgtaaagatt atgatggaat gagtaaaaaa gccgctgaaa tgatagcagc
    16441 tcaaatagtt ttaaaaccaa attcaatact gggactagct acaggaagta caccagtagg
    16501 tatgtataga gatttagtaa aaaaatacaa tgataatata gtagattttt cagatgtgat
    16561 gagctttaat ttagatgaat attataagtt accaatatct aatgaccaaa gctatgatta
    16621 ttttatgaaa gagaatctat ttaatcatgt aaacataaag ccagaaaata ctcatctacc
    16681 aaatggaatg gctgatgata ttgaaaaaga gtgtatgaac tatgaagctt ctatagatgc
    16741 agctggtggt atagatgtac aagtacttgg aataggtcgt aatgctcata taggttttaa
    16801 tgaacctgat actaaatttg caaaaagaac tcatgtagtt gaacttacag agtctacaat
    16861 agaagctaat gcaagattct ttaaatcaag agaagatgtt cctaaaaaag ctgtaagtat
    16921 gggaataggt tcaatattga agtctaagaa aatattgctt ttagcttcag gagaagaaaa
    16981 agctgatgca gtatataata ctgtttacgg agatataact ccagaagttc ctggttctat
    17041 acttcaactt cataaagaca ctatagttat agtagatgaa gcagcagcaa gtaaactgaa
    17101 tccaaaagat tataaattag tataagaata gaatgtagtt taagtaaaaa atagctatca
    17161 ttgagtaatc aaatttattt aatgatagct atttttattt aaatttttta cttcttttta
    17221 tttaaattag ttattattgt ttaaattact tgcttattac tttttacgaa aactcgttat
    17281 caagaagtat ccaaataaaa tgttaaaaat tattatgttt agtgggattc tatcaagcat
    17341 aataaaatta cctataattg ggatattacc aactggaaac ataaagaaga atacaggcat
    17401 cagtgatagt aaaaaagatg gaaaacaaag aagaagtaat cttggaatat caatatacca
    17461 actaccatcc tttttgtatt ctctaatcaa taattcagtt cctagtaata aaccaatacc
    17521 accagaggaa attaaattaa taaaaatgta tcttttaaag tcttgcatat aaccagtgtg
    17581 aaattgagta tagaaatcat aatttaattt aacggctaaa aaatacataa gcaatagaat
    17641 gattaaaaat aaaagatatc taagagcttt tttcatttta aattctccct tcagaaatac
    17701 tttattcagt tttattttgt tggaatctga ttttatcatt tagtttaaga aatccataat
    17761 attagtgaag aatgaaataa gtattatata tgaataaata tttttagaat taaggtgaac
    17821 tgtagtgctt aatataacta taatattaat tgtttgaata ttcaataaaa agtttgaaaa
    17881 atatcaattt tgatataaac tgaataaata cagttgaata ttaaattatt attttatcaa
    17941 aaatatgcaa agtactacac cttcaaatat cacctaaagt aattaaaagg caattaaagt
    18001 gaagtagttc aagtatttct aaaataagtt taataaattc tataataatt tattttttgc
    18061 cttaaaattg ccttaaaatc caaacaattt taaaactaat gtactataaa aagctggtat
    18121 aatttaaata tagtaaaaaa taagatttat cttatctatt gggatttaat tatttaatca
    18181 atatatttat accatttaat ttaaataaaa agtctattaa ggagtgagct tatgagtaag
    18241 ttaatttata tagcagatga tgaagataat ataagaaatt tggtaaagac attcttaaaa
    18301 aatgaaggtc atgatgttat ggattttaaa acgggtgatg aacttttaga acaatttaat
    18361 ataaaggagt gtgacttagt aatactagat ataatgatgc ctggctcaag tggttttgaa
    18421 gtatgtacaa aacttcgtga gaaaagtaca gtcccaataa tcatgcttac tgcaagagat
    18481 acagatatag attatattac agggataacc ttaggtagtg atgattattt tacaaaacca
    18541 tttagcccaa tgtctttggt tatgagagta aaatctatat ttagaaggat agagtttgaa
    18601 aagaaacaaa attatgataa atatagtaac tcgatagata tggaattaaa atttggtgat
    18661 gtaataataa ataaaaagaa taaaatcgtt acaagtaaaa atgtaaatat agaccttaca
    18721 ccaaatgaat ataacctatt gacttatttg tttgaaaata tagatagagc agtttccaga
    18781 gatgaattat taaacaaaat atggggatac gatatagaag ttgagacaag ggctgctgat
    18841 gatacagtga agagattacg taaaaaaatt ttaaatacaa atattttaat agaaactgta
    18901 tgggggtttg gatttaggtt gaaggagaaa agttaagatg aagaatggaa atgacaagaa
    18961 tagaattgga aagattaaat ccaaatttaa attaaagttc tcaaagaaaa atataagcat
    19021 aaaaactagg attttgttga gtatactttg tttgataatt atagtattta tgataatatt
    19081 agtatctttt aatgtatttc ttgatacata tataaaaaca actgcgaatg aagaattgac
    19141 aaagtctaca gaaatagtag aacatatgga tagtaatttt agacctatta agccaccaca
    19201 agatgatgga aagcttcctc ccaaagtatt atctaatttt atgagaaatg tacaagataa
    19261 agttaggatg gcagaaacac aatcagatgc tgatgctatg gtagttgatt ctaagtataa
    19321 cctgatattt ccaacaagag aggatgattt cttaaaaaat gttgatgaga tggagctgat
    19381 tagggagtgt attcaaaatg aaaaattaga tttaaattca gatgaaaata cacggatatc
    19441 taccagaaat aaggattatt atatttcaac tgtaaaaatt actagtatat atggtgaaca
    19501 agacaagtat cttatacttt ttatagacat atcaaaaact ctaaatctag cacaaaagat
    19561 aaatattgta ttaatatctg taatgtgttt tgctggaata ttagcaatat ttacagctgt
    19621 gattttatct gagaaaattg ctaaaccgat aaaagagttg tgtgagtttg caaaaaaaat
    19681 gggtcaagga gatttcaaaa gaacctactt tgacttttca gataaagaat tagtagagct
    19741 ctcaactgtt atgaataaga gtgctgagta tctggataag tatgataatg aacaaaaaat
    19801 attttttcaa aatgcatctc atgaattaag gacaccactt atgtctatta aaggttatgc
    19861 agaagctata aaatataatg ttatagatag taaacatgca agtgatataa ttcttgaaga
    19921 aagtgatagg ttaagtgata tggttgaaga tttattatac atttctaaaa tagataatat
    19981 aactaaagat tttgaacttg tagaatgtga tttaagagaa gtactttcaa attgtggagc
    20041 taggcaaaat gtaagagcta taaataaggg gatagaattt atttatgaat ttgatgaaga
    20101 aatggtttta tttgaatgtg atgaaaaaaa catttccaaa gcatttatga atcttataga
    20161 aaatgctttg agatacgcta agagtgaaat aaaaatagtg tgtaagtata atcaaaaaaa
    20221 tatagttgtg ataatagaag atgatggaat tggaatcaag aaagaagatt tacctcatgt
    20281 ttttgaaaga ttctataagg gagtaggagg aaatcatgga attggattat caatagttaa
    20341 atctattgta aataagcatg gtgggcgtat atacgtagaa aatggcaaaa aaggagctaa
    20401 atttactata ttatttaaat tgtaaaaaaa ttagagaaaa tatagagatg tttgaagttg
    20461 ttttttatat atatctgttg tatactgttg aaaaaagaac aatattaagc tgacatatat
    20521 tacgatagtt gacatttaaa gtaaatgctt aaatatagat acgaaaggaa aagtaaaatc
    20581 tgaagtgtat ttaggtttaa gtaagcttct tattttataa aaaatatgta tgaaagaaca
    20641 gctgattttt aatattacaa atttagtaac ggttgtattt gaagcttttg tcatccatat
    20701 gtttttatca gactttttag gccagaaaga agggtatata aaagttgtaa gatatgctaa
    20761 actgggtttt ataatatgtt taggattctg taatttaata actttaaacc ctaaaatcac
    20821 aatgccttta attttcatcc ttattttttc aacatcattt ttatataaag gaaatttaaa
    20881 gacacgattg tttactactg ttttattaag tatattcttt atactgtcag aaattgtagt
    20941 tacaagtatt tttgttttat ttgtaaaaga aggctttgaa ataatgcttg aaaataatag
    21001 tataagagta ttggcgacaa ttttatctaa aattgtcttt ttgttgactt gtaaaattat
    21061 ttgtctattt aaaaaggatg tacatttaga tatgcccata aagtattggc tccctttatt
    21121 tttaatacct atatttagtt tatttctgtc tgtttctatc tttgatgtaa gtaaattttt
    21181 tagtcttgaa agtttaaaat ttctaagctt aatatctagt gttggtattt tatatattaa
    21241 ttttattgtc ttttatttat ttaaatttat tatagataaa actaaactat ctatgaaata
    21301 tgaactactt gaaaaagaaa taattcataa ggaagaatta agacttagta atgagtgtta
    21361 taaaattatt gttgaacaga ctgattctgt cgttttcgaa tggaatatca aagaaaataa
    21421 atcatttgta tcgcaggctt ggactgaaaa atttggatat aacaatgcat gtaagaatat
    21481 ttttaaagaa attaaagata aagacttagt tcattctgaa gataaagcta tatttgaagg
    21541 ttttcttgaa agtattaaaa aaaagaatat gcacaatcaa gcagtatatc gtttaaaaaa
    21601 atctaatggt gaatatattt ggtgtagaac atcaattaca agcatttata atgatgaaaa
    21661 tgaactttta agagttgtcg gagtaattgt agatgtagat agtgatataa aaaaatatga
    21721 agagttaaga actagagcag aaagtgactc attgaccaat atatacaata aaggtacatt
    21781 tgaaaaatta gtggaagaaa ctatagttat gaatacaggt gataaaaaag atgcattatt
    21841 cattattgat ttggatgatt ttaaagaaat aaatgataat tttggtcatc catttggtga
    21901 ttttgtacta aaaacatttg ctgataagat acaaactagt tttggtagta aagacttagt
    21961 cggaagaatt ggtggagatg aatttgttgt atatatgcaa gattatgtta cagaagtaaa
    22021 tttgcataaa aaagctaaag aattaaatag agtactttcg gataattaca ctgatttgag
    22081 ttttagtttt gatgcttctg tcagtatagg tattgcaagg tatcctcaag atggaacttc
    22141 attttttgaa ttgtttaaaa atgcagatag agcactttat agcataaaag catcaggtaa
    22201 aaattcatat tgtttatttg aagaagaact atatgtacag tgaatttgaa tttagatttt
    22261 atgtgagagt aagatttata tatacttgtg gctgtctgat ttataaaata tgttactttg
    22321 tatattttta tatgtttcag taaggttaat taacataagc aaaggtgtaa gtaaatttat
    22381 attagtttaa gtttacttac acctttataa aaatacaaat aatagattca tttacattct
    22441 taaatgatta aagctcttta ccttctaaac atgcataaca tatcgttata attccagtaa
    22501 ataagactaa aatgccattc ctaagattac agtttcatca tctattactc caaatgtttt
    22561 taaagtaact tatttcgctt tgtatcataa taatttatct ccttttacat tttaacttct
    22621 gctgagattt gagttagata ttcgttagaa tctcgcacac ttatatcata tagaagatat
    22681 tcttcataaa caaaatcccc aatacgtaaa ttattctgat taaaaaaatt taataacttt
    22741 tcataagtct tgtaaatagt ttcataactc cctttatgat aagcagttac ataaagtccc
    22801 tttggtttga ttgatacagg atatttcttg tactctttat taacttttgt aaataaatat
    22861 gaatatgatt caaagtcttt attaattata tcttcacctt taatgattga gccaatcgta
    22921 ccataatcac tcaattcata gttgtccata aagttaatac attcatataa aaaattttct
    22981 tcctcatctc ctaaaaattg ttctttatat gtaacaggtg ttttgaccaa gtactcttct
    23041 tcatgttctt ttaaagtaat agtattcgca tctatattac aggcattttt agtgaatgaa
    23101 atagtagatt ccattagagc ttgaatatta tttagttttt caatttcatt tttaatatca
    23161 atggttttct tttctaaaag ctcaattagt aaattaggtg ttcttttgtc tatataagct
    23221 ttaatttctt ttaaaggcat ttttacttcc ctaagtgaag aaattatacc aaaaatgcta
    23281 agttgatggt atgagtagta tctatatcca ttttctttct ttaattctgg tgagaaaaga
    23341 cctatatcat cataatggaa aagtgttttt ttattgattc cacaaatctt tgcaaattct
    23401 ccagttgtaa aataattttt tgaaacttga ctcataatta taattcctcc ttgactatcc
    23461 tgttacagta tagtttatca tagtattttg taaaggagtg tgattatatg aaaaaattaa
    23521 ttcatttttt aaaaccatat agagttttaa ttgttgtagt tttgatattt acgtttttac
    23581 aaacacttgg aactttgtat ataccaacac ttacagcaaa tatagtaaat aatggtgtag
    23641 taaagggaga tatagattat attgtaaaaa caggtttaat gatgatgata gtagctggta
    23701 taacagcttt atcagctgtg ttagtttgta aagtatcagc aaatctttca tctggatttt
    23761 gtagagatat tagagaagca gtatttataa aatcacaaga tttatcaata aatgatttta
    23821 ataatatagg tacagcatca atgattacta ggagtactag tgatattact ttaataggtc
    23881 agtctgtgtt tatgtttatt caattggtat taccagcacc aattataact gtttctggtc
    23941 tattcttagc atattctata gataaagcaa tgacaattat tattgttgta gtaatgtttt
    24001 tattcatgct ttcagcattt ttagttggta aaaagcttat aaagttgttt aaaatgatgc
    24061 aaataaagat ggacaatatg aatagggtgt tacgtgaggt agtaactgga gtaagagtta
    24121 taagagcttt taataggagt cgttttgaaa aaaagagatt tgatagaact gcaatagatt
    24181 attctgaaac agcaatttca ataaataaaa tatttgcagt acttatgcct attgtaatgt
    24241 taataatgaa tttaggtata gtaagtatta tttggtttgg tgggatgagg gtcagtaatg
    24301 gaaatatgga aataggtcat ataatggcac ttgttgaata ttgtatctta attttattct
    24361 atttaatcat gggagtaatg gtatttatgt acatacctag agcaggtgca tgtgcagata
    24421 gagtaaatca gatattagat atagaacctg aaattgtaga tggaaatggt cataaagata
    24481 ctgtctcaga gagaggacat ttagtattta aaaatgtaac cttttcatat gcacaatcag
    24541 aagaacctgt gttaaataat ataacatttg aagcaaaatc tggagaagta actgcaatta
    24601 taggaagcac tggctcaggc aaatctacta ttgcaaatat aataccacgt ttttttgaaa
    24661 ttcaatctgg agaaatatct ataaatggtc aggatattaa gaagattcct caaaaagaac
    24721 ttagagataa gattggattt gtaccacaaa aagctttttt atttagtgga acaatagaag
    24781 aaaatataag gtatggaaaa gaagatgcaa gtattgaaga agtaaagcat gctgcaagta
    24841 tagctcaagc tgatgaattt atttctgata tggaagacaa gtatgactca tttgtagcac
    24901 aaggtggaaa taatttgtca ggtggtcaaa aacagagaat atccattgca agagcgttag
    24961 ttagaaagcc tgaagtatat gtctttgatg atagtgtttc tgcattagat tttaaaacag
    25021 ataaaagact tagaaaagcg ttgaaaaatg aaatcaaaga ttcatcagct ataattatag
    25081 cacaacgtat aagtacaatc atggatgcta atcagattat agttttaaat gatggaaaaa
    25141 ttgtaggtat tggaaaacat aaagatttac ttgaaaattg tgaagtttat aagcaaattg
    25201 cggattcaca acttagtaaa gaagaacttg cataaaatga agaattaatc tgatatttgg
    25261 aggtgtatga tatgagtgaa aaaagaatag atgaacaaga tatagaagta cttagttttg
    25321 aagatggcat gtcagcagaa aaagctaagg atgcaaaaaa aactactaag agattattaa
    25381 aatatatggc aaaacaaaag tttaaaatat taattatttt tatttcagta ttgataagtt
    25441 cagcgcttac agttttagca cctatggtta tgggaaaagc tattgaccaa ctatttaatg
    25501 gaataaaaac tgcagtccaa acaggtacaa aattcagtgt gaattttagt actatgggag
    25561 ggatagtatc aatacttcta gggttgtact taattagtgc agtatttata tatattcaac
    25621 agtatattat gtcaggagtt gcacaaaatc tagttcttag catgagaaaa gatttaagtg
    25681 ataaactcaa taaacttcct cttaagtact atgattctca taaaaagggg gaaacactta
    25741 gtatagtaac aaatgattta gaaaaagtag cagatagttt acaagaagga ttaatgcaac
    25801 ttattacagc agtagttaca gtagttggtt ctattgtaat gatgataagt ataagtgtac
    25861 cacttacaat agtatcagct ataacattac ttgtttcatt gggtattact gttgtaattg
    25921 caagaaaatc acaagaaaga ttctctgaaa atcaaaaggc actaggagag ttaaatagca
    25981 atatagaaga gatatttact ggtcaaatag tggttaaagc atttagtaaa gaaaaggata
    26041 ctataagaaa ctttaaaata ttaaatcaaa atctttataa tgcaagtaga aaagcacagt
    26101 tttcgtcata tgcaatatct ccaattatta gatttataaa cctaattggc tatgttatta
    26161 tagcagttgt aggtgggata tttgcatcaa ctggagcaat gactcttggt agtatacaag
    26221 cgtttattca atatgtaaat caagcatcag agcctactac agaaatatct tatattgtaa
    26281 atatgcttca agcagcaata gcatctgcag agagagtttt tacagttatg gacgaggtgg
    26341 aagagataaa ggatgaagaa tcttcaaagg ttattagcat gcctaaaggt aaagtacaat
    26401 ttgagcatgt taaatttgga tatagtgatg attttattct tatgaaagat attaatatca
    26461 atctaaatgc aggagataag attgcaattg ttggacctac aggagctgga aagacaactc
    26521 ttgtaaattt acttatgaga ttttatgaga tacaaggtgg aagaattact attgatggtg
    26581 ttaatataaa agatttaaag cgtggagaac ttagaactat gtttggtatg gttcttcaag
    26641 atacatggct gtttaatgga agtataaaag aaaatattgc atatagtaag tcagatgcca
    26701 caatggatga aattgtaaga gcagcaaaat cagcaagagt agaccatttt ataaggactt
    26761 taccacaagg gtatgacact atattgactg aggatgcttc taatctatct caaggtcaaa
    26821 agcaacttct aacaattgcc agggcaatac tttcagaccc atcagtattg attcttgatg
    26881 aagcaacatc tagtgtagat actagaacag aggtagaaat tcaaaaggca atgaataact
    26941 tgatgaaggg aagaactagt tttgttattg cacatagatt atcaactatt cgagatgctg
    27001 atttaatatt agtaatgaaa gaaggaacta ttattgaaca aggaagccat aaggaactta
    27061 ttgaaaagaa aggattctat gaagaacttt ataacagtca atttacaagt gaatatgatg
    27121 aagatgttgt atgattatca agttttaggc aactaaatga ttgataaata ttaagttaaa
    27181 tatagtaaca tgtaaggtta tatggaataa gagtatttct ctatttcata taaccttttt
    27241 tatttatcaa aataagataa aatttccttt ctaaattaga ttgaagaatg tatttatcaa
    27301 tataaaagta aaataatcat tattattagt gaagtaaata ttgtttgcca catatatgcc
    27361 ataaagatgc acttatttta cggagattta acaaagtagt gtaaagtata ctctaaatat
    27421 aaaataaatc aaatagagtt aaataaattg agaggtgata taaatagtga ataaaaaatt
    27481 gaactataga tttgaaatga agcacaaaat aactgaagca gatgtgttag ctttaaaatc
    27541 aagactctat ccaataatga agaaagatga aaatgcatct aaagatggaa aatatcttat
    27601 taggagttta tattttgata ctcctgaaga taaagcactt ttggataaat taaatggcgt
    27661 tgcaataaga gaaaagttta ggataagatt ttataataat gactgctctt atataaggtt
    27721 agagaaaaaa atcaaacatt acaacatgac atcaaagcta tcaactaatt taaccaaaaa
    27781 agaagtaata aatatactaa ataatgatat tgaatttcta aaagagtcga caaattgttt
    27841 attaagagaa ttttatttga aattaaaatg tgaaagatta gaggcaaaaa gcatagttga
    27901 ttacaataga gaagctttta tttatccagt tggaaatgta agagttacaa tcgatagtga
    27961 tattaaaact tctgtaaatt cggtagattt atttaataaa gatttgccaa ctatttcggt
    28021 aattgatgag aatatgactg tattggaggt taaatatgat gaatttatac cagattttat
    28081 aaaggattta attcaaatta ataagactac ctctacagca gtttcaaagt atgcttcaag
    28141 tagattatat atttaagaat aaaggctatg atttatataa agatatacat aaataaggtt
    28201 aaaaaacaaa taagttaaaa ataaataaaa taaaaaataa ataagttaaa aataggaagg
    28261 atgattttaa ttatgacatt taatgatatt tttaaatcaa gttttataga aaacgtatca
    28321 ggtttttcat ttgtggacag tgctttagca ctaggttcag cttttttagt aggtttgttt
    28381 atatatatgg tctataaaaa gacttatatg ggaattatgt attctcgacc attcaatgtg
    28441 tcgcttgtag cacttacaat gcttacaact tttgtaatat tggcggttac ttcaaatgta
    28501 gttttatcct taggtatggt tggtgcttta tctatagtaa gatttagaac agcaattaaa
    28561 gacccaatgg atttagtatt tctattttgg tcattaggtt caggtatagt tttaggagca
    28621 ggtttgattc ccttagctgt catgggttca ataattatgg gattgattct aatattcttt
    28681 tcaaataaaa caatatctga aactccatat atattgatgg taaattgtaa taatgaagat
    28741 tcagaagata tagcaactga taaaattaaa aaagtattta ctaaatatca aataaaatca
    28801 aaaagtgtta caccagaaaa aggaatagaa ttagtgtttg aagtaagaat gaaagatgga
    28861 gaaacaagtc ttataaatga cttgagtcaa gtagatggag ttactaatgc agttcttgta
    28921 agttataatg gtgactatgt agcatagaga ggagtaatct tttatgaaag ataaaaaatt
    28981 tacccttctt atctcgatta tgattatatt tttatgtgct gtagttggag tttatagtac
    29041 atctagcaac aaaagtgctg atttatatag tgatgtatat attgaaaaat attttaacag
    29101 agacaaggtt atggaagtta atatagagat agatgaaagt gacttgaagg atatgaatga
    29161 aaatgctata aaagaagaat ttaaggttgc aaaagtaact gtagatggag atacatatgg
    29221 aaacgtaggt ataagaacta aaggaaattc aagtcttata tctgtagcaa atagtgatag
    29281 tgatagatac agctataaga ttaattttga taagtataat actagtcaaa gtatggaagg
    29341 gcttactcaa ttaaatctta ataactgtta ctctgaccca tcttatatga gagagttttt
    29401 aacatatagt atttgcgagg aaatgggatt agcgactcca gaatttgcat atgctaaagt
    29461 ctctataaat ggcgaatatc atggtttgta tttggcagta gaaggattaa aagagtctta
    29521 tcttgaaaat aattttggta atgtaactgg agacttatat aagtcagatg aaggaagctc
    29581 gttgcaatat aaaggagatg acccagaaag ttactcaaac ttaatcgttg aaagtgataa
    29641 aaagacagct gattggtcta aaatcacaaa actattaaaa tctttggata caggtgaaga
    29701 tattgaaaaa tatcttgatg tagattctgt ccttaaaaat atagcaataa atacagcttt
    29761 attaaacctt gatagctatc aagggagttt tgcccataac tattatttat atgagcaaga
    29821 tggagtattt tctatgttac catgggattt taatatgtca tttggtggat ttagtggttt
    29881 tggtggaggt agtcaatcta tagcaattga tgaacctacg acaggtaatt tagaagacag
    29941 acctctcata tcctcgttat taaaaaatga gacatacaaa acaaaatacc ataaatatct
    30001 ggaagagata gtaacaaaat acctagattc agactattta gagaatatga caacaaaatt
    30061 gcatgacatg atagcatcat atgtaaaaga agacccaaca gcattttata cttatgaaga
    30121 atttgaaaaa aatataacat cttcaattga agattctagt gataataagg gatttggtaa
    30181 taaagggttt gacaacaata actctaataa cagtgattct aataataatt ctaatagtga
    30241 aaataagcgc tctggaaatc aaagtgatga aaaagaagtt aatgctgaat taacatcaag
    30301 cgtagtcaaa gctaatacag ataatgaaac taaaaataaa actacaaatg atagtgaaag
    30361 taagaataat acagataaag ataaaagtgg aaatgataat aatcaaaagc tagaaggtcc
    30421 tatgggtaaa ggaggtaagt caataccagg ggttttggaa gttgcagaag atatgagtaa
    30481 aactataaaa tctcaattaa gtggagaaac ttcttcgaca aagcaaaact ctggtgatga
    30541 aagttcaagt ggaattaaag gtagtgaaaa gtttgatgag gatatgagtg gtatgccaga
    30601 accacctgag ggaatggatg gtaaaatgcc accaggaatg ggtaatatgg ataagggaga
    30661 tatgaatggt aaaaatggca atatgaatat ggatagaaat caagataatc caagagaagc
    30721 tggaggtttt ggcaatagag gaggaggctc tgtgagtaaa acaacaacat acttcaaatt
    30781 aattttaggt ggagcttcaa tgataataat gtcgattatg ttagttggtg tatcaagggt
    30841 aaagagaaga agatttataa agtcaaaata agattaaaat tgaatataaa taattttaat
    30901 actgttaagt ctaaatattt acttagcagt atttttttag aaaataatgt atttggttaa
    30961 taatataagg taataaattg ataaaaatta aatacagcat attaataata cataataaag
    31021 taataaaaaa actgtttaag tataataact tatatttaga cagttttttt catttataaa
    31081 taatataaat gaaattgaaa acatttgaag gatagaaatt ggttggataa atgtataaat
    31141 tacttcacca aaaaataatg acaaaaattg taactaagtt gtaataaaat ggtagttatt
    31201 tagcataaaa taatgtatca tagaagtata ttataaaatt aataaaataa gatttttgta
    31261 agaaaactgc tggcttttga tatttagagt gagtagttaa attatgttta ggaggggtag
    31321 aatttgttgc aaaaagtatt atggaaattt ttaaagctag tagtcggttt atttatatgt
    31381 tcagttggta tagtattgac gataaattgt aaccttggat tatctccctg ggatgtattt
    31441 catcaaggac tttcaaatca tataggaata actataggaa ctgcttctat aattgtaggc
    31501 tctatagtag ttattgctga tgtcgttctt ggagaaaatg taggatgggg aactgttttt
    31561 aacatgctac taattggatt ctttatggat ttacttttat atagtaattt aattccagag
    31621 gctgatagtt tatttgtagg aataattatg ttgatattag gattggttct tttgtctgtt
    31681 ggaatggtat tttatatggg aagtggttta ggaagtggac ctagagatgg tttgatggtt
    31741 gcaattcaaa agaaaacagg aaaatcactt aaattaataa gaggtactat agaagttgga
    31801 gcattaatag taggattttt attaggtgga aaagttggta taggaactat aataagtgca
    31861 tttggattgg ggtatttcac acaaatggta tttggcttat ttaaattaga ttgtagtaag
    31921 attaaacata gatttatagt agatgatata aaatttatca aagtgtatat gagtggtgag
    31981 aaaaaagctg ctgttcaaaa tatagttaag taatttaaga ataaattcaa cgaaatgtta
    32041 agtattttaa attaacattt tgtttagata gataaattga atattaaaag aatattaaaa
    32101 tatgaggatt gctagaacac tataaaatat agatttctag caatttttta tttgttcaat
    32161 atttttttag tactagaagt cttattctct atttaatgaa tgaattaatt tataattaga
    32221 gatactaaac tttatagata ttaatataaa cacctttaaa ctgtgtattt tccaaagcct
    32281 ttaaaatgct tgtatttatt ctaaataaaa aaataaaaaa atgtcaaaaa aactcttgta
    32341 ttatatccaa attttatata taattaccta tgttgtttag aaattttaaa ctcaatgttt
    32401 agtaaacaaa atgtttagta aaataaaaca gaaactagga ggtgcaacat ggatataggt
    32461 gaaaagataa aaagattaag aacagaaaaa caactgaccc aggaagagtt agctaataga
    32521 tgtgaattgt caaagggatt tatttctcaa ttagaaaata acttaacatc accatccata
    32581 gctaccctta tagatatact tgaaatacta ggtacaaatt taagggaatt ttttaatgaa
    32641 atagatgatg aaagaatcag ttttacaaaa gaagatatgt ttgaaacaga agatgaagat
    32701 ttaaaatata aattaaagtg gttaattcca aattcacaaa aaaatgaaat ggagccaata
    32761 ataataacat tgtatccagg aggacagtat aaagaagaaa agccacatga aggtgaagaa
    32821 tttggatatg tattagcagg ttcaatatat gtccatatag gcgaaaagaa aaataaagta
    32881 aaaaaaggag agagttttta ctttagaccg aaagcaaatc actatatatc aaacgagggc
    32941 aaaactacag caaaagtgat ttgggtaagt acgccaccat cattttagaa tagaggtgtt
    33001 taataattgg tagaaaatat aatagaatta agggatatat ctaaacatta tgaagaactt
    33061 actgttttag ataatttcaa tttagatata aagaaaaatg agtttctaac attacttgga
    33121 ccaagtggat gtggaaaaac tacaacatta aaaataatag caggatttga gtatgctgat
    33181 gatggtaaag ttttatttga aggtaaggag ataaataatt tgccaccata tgaaagacaa
    33241 gtaaacacag tatttcaaaa atatgcttta tttccacaca tggatgttta tgaaaatgta
    33301 gcttttggat taaaaattaa aaagactcca aaagatgtaa tagatgctaa agtaaaagag
    33361 gttttgaaac ttgttgcact agaaggtttt gaaagacgac aagttgaatc attgagtgga
    33421 ggacaacaac aaagaattgc aatagcaaga gctctagtta atgaaccaaa ggtattatta
    33481 ctagatgaac cacttggagc acttgattta aagttaagac aagagatgca aatagaatta
    33541 aagagaatcc aaaaaaaatt aggaataaca tttgtttttg taactcatga ccaagaagag
    33601 gctcttacta tgtcagatac tattgttgta atgtataagg gcaaaattca acaaatggga
    33661 acaccacaag atatatacaa tgaacctaaa aattcatttg tagctaagtt tataggtgaa
    33721 agtaacatat ttgatggaat tatgatagaa gattataaag ttaatttctg taatagagat
    33781 tttgagtgtg ttgataaagg attcgaaaaa aacgaaaata tagaagtagt aataagacca
    33841 gaagatataa aaatggtaaa gccagaagaa ggaatgttaa aggggaaagt tacatctaca
    33901 gtgtttaaag gagtgcatta cgagatagaa ttgaatgaaa atggaagatt gtggatactt
    33961 cataatacta aaaatgctga ggttgggact gaactaggca tggatatata tccagaagat
    34021 atccatataa tgagaaaaga gaataactaa gatgaaaaga aaatcttttt tagcatatcc
    34081 atatgtagtt tggagtgcga tatttgtaat aattccttta atattagtag tattttttag
    34141 ttttacaaaa gaatcaggtg gaggttatgc ctttacactt gagaattata aagaagtaat
    34201 tgaccctata tatatgaaag tttttgggcg ttctattctt ttagcaggag gagctacatt
    34261 aatatgcctt atagttggtt atccagtggc atatataatt tcaaaggctc gtgtaagtag
    34321 aagaggttca ttaatattat tgtttattct tcctatgtgg atgaactttc ttttaagaac
    34381 atatgcatgg gttgctatat taggaaaaaa tggactttta aatacatttt taggttggtt
    34441 tggaatacaa ccattagcaa ttttgtatac taattttgcc atacttttag gtatggtgta
    34501 taacttttta ccatttatgg ttcttccaat atatacagca ctatcaaaga tggataatga
    34561 tttgataaac gctgctcatg acttgggtgc aaataatatg acagttttta gaaaaattat
    34621 atttcctttg agtttgccag gtgtaatgtc tggaataact atggtattta tgccagcagt
    34681 cactactttt gcaatatcaa gactgttagg tggaggtaaa ataatgctgg ttggagattt
    34741 gattgaacaa cagtttacag ttgttggaga ttggaacttt ggttcagcta tatcgatatt
    34801 tatgatgata gtaatattaa tatctatgtc tataatgtca aaatttggag atgaatctga
    34861 caaagaaggc ggtgggttac tattttaaaa ttaaagaaaa taacttctaa tatatattta
    34921 gcattagtat tcttattttt gtatgcaccc atatttgctt tggtactatt ttctttcaat
    34981 gattcaaaat caatggcaag atggaatgga tttacatgga agtggtatgg tcaattactt
    35041 caaaatgaga gtataatgag tgctttatat tatactattg taatagctat attagcgtct
    35101 gtaatatcta caatagtagg aactataagt gctataggaa tacataagat gagaggtaag
    35161 agtaagaaat taatactaaa tgtaaactat cttcctattt taaatacaga gatagttaca
    35221 gcagtagctc ttatgagtct gtttgtattt gtaaaaatgg aatttggatt tacaacaatg
    35281 ctattggcac atataatgtt ttgtttgcca tacgttatac tttcagtttt gccaaaaatg
    35341 aagcaattac ctgataatat agaagatgca gctatggact tgggagcaac acctatatac
    35401 gctcttagaa aagtaatttt accacaaata aaaccaggta tagtttctgg atttttaatt
    35461 gcatttacta tgtcaataga tgactttata ataagtttct ttaatgctgg aaatggtgta
    35521 agcaatcttt ctatagaaat ctatggaatg gctagaagag gtataaaacc tgaaataaat
    35581 gcattatcaa caataatgtt tgcagtggtt ctaggtttac ttttactagc taataaaaaa
    35641 gaatctatag taagggggat taaataatga ttaagtttaa aaaagcaata tcccttatag
    35701 ctgtaggtat gatgacagtt actttattta caggatgtaa taaagcaact gattctacaa
    35761 aagttttaaa tgtatataat gttggagatt atatagatga gtctcttata gataagtttg
    35821 aaaaagaaac aggaatagat gttcaatatt caacttatga tactaatgaa atgatgtatc
    35881 aaaaagtaaa gagtggtagt acacactatg atttagtatt tccttcagac tatatggtag
    35941 agaaaatgaa aaatgaaggt cttttggaaa aattagattt taagaatata cctaatatga
    36001 aatatttaga taagagtttt ttaaatcctg tatacgacaa aactaatgaa tatagtgttg
    36061 catatatgtg gggaactctt ggaatactgt acaataagaa agaagttaaa gaccctatgg
    36121 atagctggaa tatactttgg aatccaaagt ataagggaaa tataatgatg tttgactctg
    36181 ttagagatac tatagggata actcttaaaa aattaggata tagcatgaat agtgtaaatc
    36241 caaaagaaat aaatgaagct aaaaatcttt taatgaaaca aaaggattta gtattggctt
    36301 atgtaaatga tgaaggtaag gatagattgc ttggtggaga agtggcaatg ggtatgctgt
    36361 attctggaga tgcagttaca ctaatggagc aaaatcctga ccttgattat gctataccta
    36421 aagagggaac taataaatgg gtagatgcta tgtgtgtacc tacaacagca caaaataaga
    36481 aagaagctga gctgtttata aactttttat tagacccaga gaatgcaaaa gtaaatgctg
    36541 agtacattgg gtattcaaca cctaatacag gagcactaaa attattagac ccagaaataa
    36601 ctcagaatcc tgttgcatat ccatctaaag aagtgcttga taaatgtgaa acttttgaag
    36661 atattggtga aaatataaaa ttatatgaca aggcttggat agagttaaaa tcaaaatagg
    36721 gtcttgttgg tttaagtaaa tttacttaat aaatacttaa taaaactgtt cttttttaga
    36781 aaatttattt tctaattatt gaacagtttt attttttgtt taagttatat tcatatgcaa
    36841 tttaaatagt ttattaataa atgaaaactt atttgtataa tgactttaat aataattaga
    36901 aaaaatgggc aacttaatag aataattgga ggtgtaaatt tataaaaaat gaataggata
    36961 aataaaaaaa tagatttatt aatattggct ttgctcgttt ttatattttt aattgttgga
    37021 atagtaatac tatcgattag gacagaaaat aaattgaatg tgttcataat gttagcaata
    37081 atttttttta taataatgtt aacttattta tcaaattcag tagtggggtt gataacaagt
    37141 tcaataatta tatttatgta tacatcctat attttatata acaatattac tcacaatatg
    37201 gatgttgaat ttatatcata tatgtggata atagcaacac cagtatcttc aattataatg
    37261 ggtaatctga acaaaagcat aaatgaattg caaaatacaa ataaaaaact atctgaacag
    37321 tataaggaat tagtgactat tgatagtgaa actggactta gaaatttaaa gattttttat
    37381 aatgatgtaa atatggagat aagtaaatct attagacata atactgattt ttctcttatg
    37441 atagtaaaat taccatacta cggaaaccta cagactatat ttggtgaaaa caaaactaat
    37501 aaaatagtaa aatatattgg ttcaaatatt atagaatgta caagaaatga agatataatt
    37561 tatagtttac aaaaagatat gatagggata ctaatgccaa atacatcttt ggaaggttca
    37621 aaagttgtta aagacagaat aaagaaaagg ataaaagaat taaatttaga tttaaacaat
    37681 aggggaaaat atgtaaatat agatgtcaaa attgcatttt tacaatataa gagtagtttt
    37741 ggagatagta taaattttaa gaatatagtt gaagaggaac ttcaatatga tgtttaaaat
    37801 atttaataaa agtctattaa aattagtagt tgtattatca atagtactga caatatcaaa
    37861 ttttagctta tcttcatatg catcagaaat tacttccagc acagataagg tattaatcat
    37921 atatgattct aagaaagaaa cagcttataa tagagatatt ttaaatatta tgaggactct
    37981 attgggaaga tttagttcag atatagagct attaaaattg agtaactatg atggtgagat
    38041 aaataaaaat tattacagtc atatatttat tttaggaatc aatgaaaact catataataa
    38101 tgataaaaac actaaaaact tgattagttc cttaaattta tataaaggaa ctatatgttg
    38161 gttaggatat ggtatagaaa atcttttgga acataaaaaa tacaatttag actatgtagg
    38221 aaaaacaaac aatatagtta gtgtaaatta tagaggtaaa tcatataact tggatgaaca
    38281 ttatgttttt aacattgtag aatcaaaaga tacaagtaat aaagtaatag gaagtattaa
    38341 tgatacctta aataagtatc catatataat aaatgataaa aatctttttt atgtatcaaa
    38401 attagattta gatggagttt tattttatat attttgcgat tcacttaatg atatttttaa
    38461 tattaagacg tttgataaag gacgtatatt tgtaagaata gaagatgtac atgcttttag
    38521 agaaccaaaa aatcttgtag agatagcgga ttatttaagt agtaagaata taccatttac
    38581 gattgcatta ataccagcat atgtaaatcc taaaaatcat aaggtaataa ctttatcaga
    38641 aagccctgaa attgtcaaag caataaaata tatgcaagat aaaggtggaa ctgtgatatt
    38701 gcatggatat actcatcaat acaagaaaga agaagtttct ggagaagggt atgaattttg
    38761 ggatggtaaa aaagatgaac ccttaaaaga aaatatgaag atatttgtca aagatagagt
    38821 tttgaatggt cttagagtat gtatagagaa tggaatttat cctcttgcat ttgaagcgcc
    38881 acattatgca atggaatcag agggatataa agaattaaaa aaatattttt ctacttatat
    38941 agggcaacat caaaataatg ataaaaaatt tagcacaaat acatatcctt atattattag
    39001 ggatacagag gagtttaata tatttatacc cgaaaattta ggatatatag acccagaaga
    39061 taagtttact tttcaaaata taaaagaaaa tttaaataaa ttatccattg taagaggttt
    39121 tagtggaggg ttcttctttc attcatattt aaacatagag tatctaaaaa acacaataga
    39181 atatttagaa aaacagaata tagaatttat gaatttaaga gattttaata attgggtaaa
    39241 agttgatgaa atacaaataa gaaataatgg agatgaaatt atagtcaact atgataagga
    39301 cttagatgaa ctaactaaaa gtgatactag atttaaatcc atatccaata ttagtaaaat
    39361 tttaatattc atagtatcta taagtgtttt aatatttgtg attatattta tatattttaa
    39421 gagaatagat aaaaagaaat ttttaaaata aatgatttta tattagaatc aatattttaa
    39481 aatattttag gagaaattta tgagagattt aaatgtatca gagtatttgt tcgtatttag
    39541 tctgtttagt atatggtctt tattgcttat aaatattatt ttagctatgg gtggatatat
    39601 attttatttt aaaaactttg ataaagaaat aaaagaaatt gatgaatatc ctatgatatc
    39661 aatacttgtt cctgctcata atgaagctaa agtgataggt agaactgtgg agtctttgct
    39721 attactcaat tatcctaaga gtaagatgga acttattgtt ataaatgaca attcttctga
    39781 taatagtaaa gaaattttag aaaatataaa ggataggtac aacaattata atttcactat
    39841 aataaataca gatagtttaa caggtggaaa aggaaagtct aatgcattaa atattggata
    39901 tacgatttca aaaggagatt ttatagctgt ttatgatgca gataacacac cagataaaaa
    39961 tgcacttaga tatctagtac aaactatagt aatgaatgat gaattaggtg ctgttattgg
    40021 gaaatttagg actcgaaata aaaataaaaa cctattaacc aaatttataa atatagaaac
    40081 attaagcttt caatggatgt cacaagctgg tagatggcaa ctttttaatc tatgtacaat
    40141 accaggaact aactttatat tgaggagaag tatcatagaa gaaattggag gatgggatag
    40201 taaagcaatt gcagaagata cagaaataag ttttaggata tataaattgg gatacaagat
    40261 aaagcttgta ccacaatcta taacatggga acaagagcca gaaactgtta aggtgtggat
    40321 aaagcaacgt actagatggg caaaagggaa tatatatgta ttaatgaaat atataaaaaa
    40381 tatatttaaa cagggaagaa ataagattgt gtttgatata gcttactttt tttctgtata
    40441 ttttctattt ttaacatctg taattatatc cgatatttta tttgttttaa gtatatctaa
    40501 actagtagaa atttctattc caattaactt ttttctaata tggatactat cttatctttt
    40561 atttattata gaagtgagta taagccttac tatagaaaaa ggtgaagcaa caattgaaaa
    40621 tatattcata gtggcaataa tgtattttac atactctcaa ctatggctgt ttgtagctat
    40681 aaaaggaatg atagagtatc taaaagatat aatttttaaa agagaggtta aatggtataa
    40741 aacagaaaga ttttgattca tgtacaggag gaattaacat gaaaaaattt attatatcaa
    40801 ttatatcttt agtattgttt ttttcaaata tatcacttat atacaaagta aatgctgatg
    40861 agactaaagt taaaaattat aagtttgaaa gagatataac tattgatggt gttataggaa
    40921 gtaatagtac tttttttgaa gtaaataaaa attgggatat tgaagaagtt ttattgcatc
    40981 ttaattttag taagagccaa atattaaatg gagatgtttc aagtttaact gtattgataa
    41041 ataatgtacc aataaaatct attaaattaa atgctaagac aaattataaa aatacattag
    41101 aagtattagt tccaaaagat tatataattc aaggatataa tgaaataaaa ataaaaacat
    41161 acaaaacaat atctgataaa atttgccaag atgattccaa tactgggaac tggatggtta
    41221 ttcacaaaga atcatatata tcaattagat ataagcagaa aaaggtggaa aactcaataa
    41281 atgaatatcc gtatccatat gcagaaattg aaaataatca taaattagat acaactatag
    41341 tagtaccaga taatatgact agaggagaga ctacagcagt gtttaatttg gcatcagcat
    41401 ttggaaaaat aacaaaaaat gatgatttaa agttagatgt taaattatat tcagaaatga
    41461 aaaattggtc agatgataat attatatata ttggtaaacc tgaaaataca gcagaagaaa
    41521 ttttggatat tttaagtata aaggaacaaa ccctattatc ttcaaactgt ataatcaagc
    41581 aagtagattc accatacaat aagaataaga agatgatggt ggtaattggt agtaatgaag
    41641 atgatttaat aaaggcatca aatttattaa tagaaaatag actttctaac caagttttat
    41701 catcatcagt acttgtaaac aaagagacta atataaaaat taatagggaa caaaaattaa
    41761 atttaggtca tttaacatta aaagatttag gatattctga ctttttgttg gaaggagcat
    41821 ttaatcaaca agctttgttt gatgtcaaaa taccaacggg taaggtatta gatgatggtt
    41881 caaagattat cttaaattta agatattctg acaatttaga ttttgaaaaa tctttagtta
    41941 cagtgtctat aaatgatgtt attgtgggaa gtaaaaaatt ggataggtca cattctaata
    42001 atgataagtt agaattaaaa atacctaaag atatagataa taaaaactat tatcaagtta
    42061 aattgacatt taatttgagt ataaaaaatt cgaattgtgt tactagagag agtaataatc
    42121 cttgggcata tgtatcaaat aattcttatt tggcgttatc tacgaaagaa aatgaaacct
    42181 tatcttttga aaattatccg tatccatttg ttagagatga tgaatttaat gatttaactg
    42241 tgataatgcc agattattca gggtcacaag ctatgacctg gatgtttaga ttaggtgtta
    42301 ctttaggtgc gaatataaat tcacataatg gaaatataaa tgtaattaga ggaaaagaat
    42361 ttagtgacaa gtataaggat actaatattg tagtttttgg agtgccacat aataattctg
    42421 ttataaagat gttaaataat aatttaaata taaagttcga taaaaattac tccaatttta
    42481 tatcaaatga taagataagc tttattgatg attatggaaa aaatatttct accattcaat
    42541 taataaaatc accatataat aatcaaaaaa atatcatggt aattagttca atgaatgaaa
    42601 aaaacttata tttaggaatg gattatttgt tgaataaaag taaagttaat gatttaaaag
    42661 gagatacatt gataattgat gaatacggag aggtagaaga tttagcttat aatttaaaac
    42721 ctaagaaaga agtaaaagat tcaagctgga atatgagtat aaataagaca actaaagtat
    42781 ttttaatgat ttcttttatt actataattg tagtcatgat attatcaatg ctatatataa
    42841 aaaaatataa aagaagataa tatgataaat ttattatttt aaaagaggta tataatttga
    42901 ggaaatatat gaatgaattt aaattaaaaa gaagtatttt aatctcatta ataatcttaa
    42961 tttttatatt tatagtttac tttgtaagta ttgagttaaa agataggagt ctagtaaata
    43021 atcaatataa aggtaagatt aaatcagcca atttatctgt agattatgaa ataaatcaag
    43081 ttatgaagga tatagataaa ttgggattga atacagtaaa tgtacctata attataaatg
    43141 tagagtccat taattctgat attatgtcaa tagataataa tagtaaagaa aaggcaatta
    43201 agttaataaa aaaattaaat aaaaaaggta tttcaacaat attagaggca tatccatgga
    43261 ttgaaaatgg aaaattgtat gagactgact ggaaccctat aaataaaaaa agattttttt
    43321 atacttggca aaatgccata ttaaatgaac ttataattga tgtagcaaat ccactggatg
    43381 taaatgtttt aaatattggt tctaattttg tacatttgga agaatatcaa caaaattggg
    43441 gtgaaataat agattttgta caatccaaat ttgatggtct tgtaacgtac agaactaatt
    43501 ggtggtatac aaaaaaagat gatttaagta gtaagttgtt ttatgaacaa aagttgaaca
    43561 ataatttttt tgataagtta gattttatat ctatagcagc atattttgag ttaagtaata
    43621 aacctgttaa tacagtggat gaattaattt ctgccttaca ctcatctact gtaaacaata
    43681 gaggacaaaa tataaaacaa gaaatatata acctttataa agctcataga aaacctatat
    43741 tttttggaga attaggtttt tcaaatagag aaagtgcaag ttctcagccg tggaatcata
    43801 ctccttcaaa agctgtaaat ggcgaagaac aagcaaggtg ttttgaggca tataagaaag
    43861 tttttgaaaa tgaagactgg ataaatggtt tttctgtgtt ttgtgtaggt aaaatagatg
    43921 acgaaaaaaa tttttaccca tcaaaagaga gtataaaagt tattaagtct tggtatgaat
    43981 aattttgaaa gtgtagaatt tatatagatt aaatagatga ttaggagaga tataaatgaa
    44041 tgacatcaag gttatgactg tatttggaac tagacctgaa gctatcaaag tagcaccttt
    44101 aataaaggaa cttgaaaaaa gagagaatat aaatagtatt gtttgcgtta cagctcaaca
    44161 tagagaaatg cttgaccaag ttatagaaac ctttaatata aatgtagact atgatttgga
    44221 tataatggaa aaaggacaaa gcctgaatga tataacatgt aagattttaa ataaacttcc
    44281 cttgatttta aacaaggaaa atccaaatat aatattggta catggagata caaccacaac
    44341 tctagctaca agtttgactg cattttataa taaaactctt gttggtcata tagaagctgg
    44401 gcttagaacc tatgataaac attctccttt tccagaggag ttaaatagac aacttacagg
    44461 tattattgct gatatgcact ttgctcctac taatttggct agaaaaaact tgatttctga
    44521 aggaaaacct aataataata tatttgtaac tggtaatact gctattgatg ctttaaagat
    44581 gacaataaaa gaaaactata atcatcctat aattgatgag attggtaacg ataggatgat
    44641 tttgttaacc tctcacagaa gagaaaattt gggtaaacct atgaaaaata tattcagagc
    44701 tataaagcga atagtagatg actttgaaga tgtacagata gtatatccca tacatttaaa
    44761 tcctaaaata cgaactatag ctgatgagat atttggaaaa tttcctgaaa aaattcatat
    44821 tatagagcct ttagatgtag ctgattttca taacttttta aataaatctt atatgataat
    44881 gactgatagt ggaggaatac aggaagaagc tccatcatta ggtaagccag ttcttgtgtt
    44941 gcgtgataaa acagaaagaa cagaaggaat tgaagcaaag acacttaaat tagtaggtac
    45001 taatgaagat agaatatata attcagtgag cgatttatta ataaataaag ataactatgt
    45061 tcagatgagt aaagcatcta atccatatgg agatggaaat gcaagcaaat atatagtgga
    45121 tataataatt aaaaaattta attgcaaata tttaaattag ataaggccta aaacattaac
    45181 taattgatat aagatttatg ataatattaa ggaatacaat ttttaagggg gagtaatatg
    45241 gctagaaaat taataaaaaa tttgggtaaa agtaaaagtg taaagagagt gaaactttta
    45301 tttaagaaga tttttattac tgtattcata gtagcaagta tagttgctat ttttaatata
    45361 acgaaatatt ttgaggaatt atacaaagta agggatttaa aaagtactaa aattgaatac
    45421 tatatggatg tggctgatga agcaggagat ggaaaggtcc aattaagttg gaaggcctta
    45481 cttgctatag acatggtaat acatgatgaa gatttaagta atataaaaaa gaaagacaca
    45541 ttggatatag gggaaaagtt tatagtagaa gataaaaatg ataaaggcga aaaggtgtat
    45601 aaggtaaaaa agtttaataa ggtattaagc gaattgaaat ttgactcttc tcaaaaaagt
    45661 agagcaagaa aatacatgaa agatttggaa tacacatacc ttggaaataa acaattagat
    45721 agtagtgatg aaaaaattaa atttataaag aagttagaag actcagctat aagagaatat
    45781 attgattatg gaatattgcc ctctataaca attggacaag ctatattaga atctggttgg
    45841 ggaaattcta aacttacaaa acagagtaat aatttatttg gtataaaagc agataaagca
    45901 tggaaaggaa aaagtgtaga aatttcaact tcagagcatt ataatgaaaa aattgtagct
    45961 agttttaggt cgtataattc attacaagat tctgtcaaag accacagttt atttttaatt
    46021 aataataaaa gatatagaaa acatggattg tttgaggcaa aagattatat tagtcaagct
    46081 caatcattag aaaatgcagg atatagtaca gcagaagata aaaaaggaaa tcgtatatat
    46141 gcagagctat taatagatgt tattaggagt tataatttgc aattgataga taataaagtt
    46201 gagacaaaat aattgttaac tttatatgag gagataacaa gataagattc ttttctacaa
    46261 aaagaataga aaaattgtaa aaaaaactaa aaaaatgtat tgacttgtta tttaaaaggt
    46321 gatattatta ttactgtact cgaaagagaa caaaa
//
LOCUS       DAGSWI010000014        45771 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.014, whole genome shotgun sequence.
ACCESSION   DAGSWI010000014 DAGSWI010000000
VERSION     DAGSWI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.014"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            202..1419
                     /locus_tag="MJF80_000732"
     CDS             202..1419
                     /locus_tag="MJF80_000732"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBZ0279407.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1514..1894
                     /locus_tag="MJF80_000733"
     CDS             1514..1894
                     /locus_tag="MJF80_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBZ0279408.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2178..2660
                     /locus_tag="MJF80_000734"
     CDS             2178..2660
                     /locus_tag="MJF80_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBZ0279409.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSDVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2681..3496
                     /locus_tag="MJF80_000735"
     CDS             2681..3496
                     /locus_tag="MJF80_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBZ0279410.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3549..5099
                     /locus_tag="MJF80_000736"
     CDS             3549..5099
                     /locus_tag="MJF80_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBZ0279411.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5196..6548
                     /locus_tag="MJF80_000737"
     CDS             5196..6548
                     /locus_tag="MJF80_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBZ0279412.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGLHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6679..7437
                     /locus_tag="MJF80_000738"
     CDS             6679..7437
                     /locus_tag="MJF80_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0279413.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDNN
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7484..8203
                     /locus_tag="MJF80_000739"
     CDS             7484..8203
                     /locus_tag="MJF80_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBZ0279414.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8327..9727)
                     /locus_tag="MJF80_000740"
     CDS             complement(8327..9727)
                     /locus_tag="MJF80_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBZ0279415.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9731..10639)
                     /locus_tag="MJF80_000741"
     CDS             complement(9731..10639)
                     /locus_tag="MJF80_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0279416.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10900..11547
                     /locus_tag="MJF80_000742"
     CDS             10900..11547
                     /locus_tag="MJF80_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBZ0279417.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11872..14511
                     /locus_tag="MJF80_000743"
     CDS             11872..14511
                     /locus_tag="MJF80_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBZ0279418.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14569..15672)
                     /locus_tag="MJF80_000744"
     CDS             complement(14569..15672)
                     /locus_tag="MJF80_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBZ0279419.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16033..16854
                     /locus_tag="MJF80_000745"
     CDS             16033..16854
                     /locus_tag="MJF80_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBZ0279420.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16933..17144
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17231..18547
                     /gene="argH"
                     /locus_tag="MJF80_000746"
     CDS             17231..18547
                     /gene="argH"
                     /locus_tag="MJF80_000746"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBZ0279421.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18796..19134
                     /locus_tag="MJF80_000747"
     CDS             18796..19134
                     /locus_tag="MJF80_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBZ0279422.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGKP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19261..20550)
                     /locus_tag="MJF80_000748"
     CDS             complement(19261..20550)
                     /locus_tag="MJF80_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBZ0279423.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20630..21334
                     /locus_tag="MJF80_000749"
     CDS             20630..21334
                     /locus_tag="MJF80_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBZ0279424.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21426..22472
                     /gene="selD"
                     /locus_tag="MJF80_000750"
     CDS             21426..22472
                     /gene="selD"
                     /locus_tag="MJF80_000750"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21474..21476,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBZ0279425.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22490..23902
                     /locus_tag="MJF80_000751"
     CDS             22490..23902
                     /locus_tag="MJF80_000751"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBZ0279426.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            24016..25899
                     /gene="selB"
                     /locus_tag="MJF80_000752"
     CDS             24016..25899
                     /gene="selB"
                     /locus_tag="MJF80_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBZ0279427.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            26004..28742
                     /locus_tag="MJF80_000753"
     CDS             26004..28742
                     /locus_tag="MJF80_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBZ0279428.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28861..29805)
                     /gene="manA"
                     /locus_tag="MJF80_000754"
     CDS             complement(28861..29805)
                     /gene="manA"
                     /locus_tag="MJF80_000754"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBZ0279429.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            29978..31339
                     /locus_tag="MJF80_000755"
     CDS             29978..31339
                     /locus_tag="MJF80_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBZ0279430.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31427..33316
                     /locus_tag="MJF80_000756"
     CDS             31427..33316
                     /locus_tag="MJF80_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBZ0279431.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33331..33786
                     /locus_tag="MJF80_000757"
     CDS             33331..33786
                     /locus_tag="MJF80_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0279432.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            33845..34162
                     /locus_tag="MJF80_000758"
     CDS             33845..34162
                     /locus_tag="MJF80_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBZ0279433.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34187..35449
                     /locus_tag="MJF80_000759"
     CDS             34187..35449
                     /locus_tag="MJF80_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBZ0279434.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35554..36618
                     /locus_tag="MJF80_000760"
     CDS             35554..36618
                     /locus_tag="MJF80_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBZ0279435.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARNIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36672..37850
                     /locus_tag="MJF80_000761"
     CDS             36672..37850
                     /locus_tag="MJF80_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBZ0279436.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(38041..39099)
                     /locus_tag="MJF80_000762"
     CDS             complement(38041..39099)
                     /locus_tag="MJF80_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBZ0279437.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            39166..39537
                     /locus_tag="MJF80_000763"
     CDS             39166..39537
                     /locus_tag="MJF80_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBZ0279438.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            39574..40248
                     /locus_tag="MJF80_000764"
     CDS             39574..40248
                     /locus_tag="MJF80_000764"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBZ0279439.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            40497..41336
                     /locus_tag="MJF80_000765"
     CDS             40497..41336
                     /locus_tag="MJF80_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBZ0279440.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            41580..41676
                     /locus_tag="MJF80_000766"
     tRNA            41580..41676
                     /locus_tag="MJF80_000766"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41614..41616,aa:Sec,seq:tca)
     gene            41827..42534
                     /locus_tag="MJF80_000767"
     CDS             41827..42534
                     /locus_tag="MJF80_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBZ0279441.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(42676..43512)
                     /locus_tag="MJF80_000768"
     CDS             complement(42676..43512)
                     /locus_tag="MJF80_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBZ0279442.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            43700..44605
                     /locus_tag="MJF80_000769"
     CDS             43700..44605
                     /locus_tag="MJF80_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBZ0279443.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            44614..>45771
                     /locus_tag="MJF80_000770"
     CDS             44614..>45771
                     /locus_tag="MJF80_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0279444.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKK"
BASE COUNT        17946 a         4984 c         7903 g        14938 t
ORIGIN      
        1 aaatattttt aaattatcat ttaatgtaat ttaaatgaat tgaagatata tgtttattgt
       61 atgcaattgt tgtattttta ggttttttgt gattaattaa aaaattataa aaatcgttta
      121 catgttgatt tttaaaaaaa aaagatatag aattatatag aaagtaataa acaaaaattg
      181 gatttacggg agtggaatga aatgatgaat gttaccttaa cagatgtaaa agaggctaga
      241 gagaccatta agaacatagt aaaaagaact gacttacttg aaagtgttaa acttagcgaa
      301 aaaacaggag caaatgtgtt ctataaatgt gagaatcttc aaaaaacagg ttcttttaaa
      361 ttaagaggag cttgtaacaa aatagcgagt ttaacagatg aagaaaaggc aaatggagtt
      421 atagcttcaa gtgcagggaa tcatgctcaa ggagttgcat tgggagcaaa aatgactggt
      481 ataaaatcta caatagtaat gccagcaact gcgcctttag caaaagttag tgcaactaaa
      541 ggttatggag cagaagtagt tttaaatggt gcggtttatg atgatgcata tgcaaaagct
      601 gttgaaatac aaaaagaaac aggagcaact ttcttacatc cattcaatga taagtatgtt
      661 atagcaggac aaggaactat atctcttgaa attttcgaac aattagacaa taaagttgat
      721 actatactat gcccagttgg tggaggagga atcatctctg gtgttgctgt tgcagcaaaa
      781 gcattaaatc ctaatgtaaa aatagtcggt gttcaaactg caaatatacc ttcaatgaaa
      841 gagtcaataa aaaatggaaa agtaacaaca gcatttaatg atactacaat agctgatggt
      901 atagctgtta aaactccagg agatttaact tttgaaataa taaatgaatt ggttgatgaa
      961 atagtagtgg ttgaagagac agaaattgca gaatcaatat tatttatgat ggaaagtcaa
     1021 aaaatagttt cagaaggtgc tggtgctgta tgtacagctg ctatattaag tggtaagtat
     1081 gtaccagcaa aagatgaaaa tgtagtttgt ataatatctg gtggtaatat agacataaat
     1141 acgttatata gaataatagg tgttgcttta gctaaagaag gaagaagata ttcatttagt
     1201 acaattatgg aagataagcc aggaaacttt gctgagttaa ctagaataat aagtgagaat
     1261 ggtggaaaca ttttaagtgc taatcaaggt aaattatcag caggggaagc tctaggaaaa
     1321 caaagtgcgg aatttatatt agagacaata gattatgacc atatagctag aataaagaaa
     1381 gctatagaag aaaaaggttt taaaattata gaattataat aaaaagtgta tataatatac
     1441 aagaaaaata gtataatagt gtatgaagat acattttatg tgtattagat taacgaatta
     1501 ggaggatttt aaaatgaaac atgaagtaat acatactaat gatgcaccag cagctttagg
     1561 accatattct caagctataa aagctggaaa cttgttattt gtatctggac aagttcctct
     1621 tgtaccagag actatggaag tagttgaagg agatgtacaa gctcaaactg ctcaatcttt
     1681 aaaaaatctt aaagctatat tagctgaaag tggagctgat ttttcaaatg tagttaagac
     1741 tacagtattt ataaaagata tgaatgaatt tggggctata aacgaagttt atgctgaata
     1801 ttttggagaa aataaaccag caagagcttg tgtagaagtt gctagattac caaaagacgt
     1861 taaagttgaa atagaagtaa tagctgtatt ataaaaatac aatattttta atttgaaaag
     1921 gctgaaaaac tatattctag tgattcagcc tttttataaa aaaataaatc acaataaaga
     1981 acatgataaa tattgtcgaa ttaaaaaata aaattaaaat aaatattgca atatttttga
     2041 gaaggtttta taatgagtat ataatttgaa acgtgtaact attaaattag gcatgagttc
     2101 ttggatattt ttatcctgag gattcatgcc ttttttgtat tgaaattcaa aaatctattt
     2161 aataaggagg aagtactgtg ttaaattttt tcaagaaaaa taaaagctat aagttacatg
     2221 cagtagtgag tggtaacagc attaatattg agaaagtaaa cgattctgtg ttttcaaaaa
     2281 agttaatggg agatggagtt gcaattattc ctaatagtga tgttgttgta gccccttgta
     2341 atggtaaagt aactgtgctt acagaatcaa agcatgcctt tggtatggtc tcagatgagg
     2401 gtgttgaaat attagtccac ataggaattg atacagttag tttacagggt gaggggttta
     2461 aaaatgaagt gtctcaagga gatactgtta aaaaaggtag tccaataata agttttgaaa
     2521 gagaaaaaat aaattctcag ggaattgatt gtacaacaat aataattgta ttaaatcata
     2581 gcgaattttc agaaataaat tgcatggtag aaaatgaagt tgttgcaggg caagatactg
     2641 ttattgaaat tatgaaataa taggaaggga ttaattcaat atgagagtag taaaaatctt
     2701 taataataat gcattatcta cagtaactaa tgataggaaa gaagctattt tactaggtct
     2761 tggtataggt tttaataaac gtccaggaga taaagttaat gaagataaaa tagaaaagat
     2821 ttattatgtt caagaccaca tgcaaacaaa atttttagaa ttattaaaaa atgttagtcc
     2881 agaagtgatg gatgcttcac aacaaattat aagtcttata ccaggaaatg agggaaaatt
     2941 taataataaa gggatattat ctttagtgga acatattagt tttgcaatag atagaatgaa
     3001 aaataatgtt tttttaccta atttaatgct tttggatata aagatgatgt attcaaaaga
     3061 gtttgaatta ggagtaaaag ctttagaaat tatatataga gtatgtcata tccatttgcc
     3121 tgaagatgaa gcaggttaca tagctttaca ttttgtcaat ttacaatcaa atgataattt
     3181 ggcatatgat actttaaaat ttgtaaaagg tagtattgat ttgattaaag aatgttatgg
     3241 attagaatta gacgaatcga gtttaagtac attacgtttt agaactcatc ttaaatttct
     3301 tgctcaaaga atttttaaga atgaaatttg tcaggatgat aaaatgattg aaatgtatga
     3361 ttatttaatt aacaatcatc ctaaaaataa agaatattta gaaaagttaa atgtatatat
     3421 tgaaaaggag tttaggtata aattagataa acctgaaaaa atttatctac tactacacct
     3481 aacaaaaatc ttataagaat gagatgaatt agtaatagaa atatatataa ttttaggggg
     3541 ggataagatt gaaagaaaaa ctacttggag gactggaaac atttgcaaag gcaatggttc
     3601 agccattaat gtatttatcg agtgcaggaa tattaatggt ggtaggtgta ctacttacaa
     3661 atgatatgtt attaggagta cttccatttt ttaaatggga gccaattcaa atatttggaa
     3721 gcctaattta taactgtata atggctatta ttaataatct tgctgttata tttgctatag
     3781 gtattcctgc tgcactagca aaaaaagaga agcacaaagc agcgttaata ggatttatga
     3841 gttatataat ataccttaca gcttccaaca ctatgttaac atcactaggt caactagcaa
     3901 aagaaagcaa gatgcttgga ctgataggga ctggacaagc tactatttta ggtattcaag
     3961 ttgtagatac aagtgtattt ggtggaatta ttttagggtg tatagctgga tatgtattta
     4021 atcgtacaag caacaaaaca tttaaaggag ctttacagat atactcggga gcaaatttta
     4081 gttttatatg catgttcttt gtatccattt taattggagt tgtgagtaac tttatatggc
     4141 cattagctca acaaggaata gcttctcttg caaatgtcat caaagataca ggtacatttg
     4201 gtttattctt atatggtttt ttagagagaa tattagtacc aactggactt catcatttaa
     4261 tttatacacc atttcagttt tctgatttag gaggagtgct taaagttggt gcagatacag
     4321 tttcaggagc ctatgctata gttatgacag aaatgaattt accagttgag cgtttctcag
     4381 atagtatata ctatatggca actggattta caaagatgtt tggttatata ggtataggag
     4441 ctgcctttat ttatactgca tataagcaaa ataagaagaa ggttaaggca acgattattc
     4501 cactaataat tacagcagta ttagcctctg taacagaacc attagacttt atgtttgtat
     4561 tctgtgcacc acttttattc tttgtacatg cagttatagc tggtcttttt atagctttat
     4621 taaaaatatt tgatgttact gcattatgtg gagggaactt attagtatca attataatga
     4681 atactacagt aggcgtagaa aaaacacact ggccagtaat gatgatatta ggacttgcac
     4741 aaattattgt atattttgta gtatttagtt ttttaattaa gaaatttaat tataaaactc
     4801 caggtcgtga agatgagagt acagctacag gcgaagaaca atcaaaacaa cttaatttag
     4861 atgcagggat agaaaatata attgatgggt taggcggaaa agaaaatatt aatacagtag
     4921 aaaattgcat cactagatta cgtgttaatg taaaagatga aagtaagatt aatgaagata
     4981 taattaattt aacgccaaat agcgggattg taaggaaagg aaaagatata caaataattt
     5041 ttggacttca tgtgcatgag gtaagacgtg cagtagaaga ttttttagaa gcttattaaa
     5101 gatatatatt tatatagcaa gggtaaataa cagctttaag tgctgtttac tatttattaa
     5161 agatacatat aatttataga ggaggaataa ttattatgat tattactatt gttggtggag
     5221 gaagcacatt tactccaggt attgttaagt caattgcatt aagagaaaaa gagttagagc
     5281 ttgaggaaat tcgtttattt gatattgata aaaaacgtca agataaagta gctgtagttg
     5341 tcaactggat actaaaagaa gatgttaaaa cagatgttaa actaactgta actaattgtg
     5401 aaaaagaagc gtatacagat gctacattta tatttgcaca aatgcgtgtt ggtaaatatg
     5461 aaatgcgtga gcaggatgaa aaaattccat tgaaatatgg ttgtgtaggt caagaaactt
     5521 gtggttgtgg agggatggca tacggactgc gtacaataca accaatgata aaggttattg
     5581 atgatgtaga gaaatatgct aaaaaagatt attggatatt aaattactct aatcctgctg
     5641 caatcgtttc tgaagcttgt cgtaagttaa gaccaaatgc aaggattata aatatctgtg
     5701 atatgccaat agctattata gatatgatag cggggtcatt aaatatcaat gacgttcata
     5761 atatacgcta tgattatttt ggtctaaatc attttggctg gtttacttca atagattata
     5821 aacatagaga cttaatggaa gagattaagg aatacataaa agaaaataaa atattattac
     5881 caaaatctta tatagaaaac ttaaataaaa gtcgtagtac aggaaatcgc catgctgtaa
     5941 gtagctggat acatgtttgg gaatctgttt atacaatgtt agaaagcttc ccaaaatatc
     6001 tgccaaacac ttatttaaca tactatcttt taagtaaaga aacagtggaa cattcaaaca
     6061 taaatcgtac tcgtgcaaat gaggttatgg aaagccgtga aaaagactta tttgaaggtg
     6121 tgagacatta cttagaaact ggagaaattt ctgaaaaagc attttatgca ggtttacatg
     6181 gagattggat tagtgatttg gcagtttcaa ttaaaaatga tacacgtagt cgttttttag
     6241 ttataactga aaatcgtgga gctataccaa acatgccata tgatgcaatg gtagaaattc
     6301 cagcatatat tggtaaagat ggtccagaag ttattgctcg aggtccaata cctctatttc
     6361 aacaaggatt aatgatgcaa caacttaaca gtgaaaaatt gatagtagaa gcttatatag
     6421 aaaatagtta tgaaaaagct ttacaagcat tcacattaaa taaaactatt ccaagtatga
     6481 atgttgctaa agaaattttg gatgaaatga tagaagttaa taaggaatac tggccagaat
     6541 taagatagta ggataaaaga aagggttttt atttaatcat tagatatgtt ttcagagtct
     6601 tttatcaaaa tgtattcaca aacatatggc aatatattaa aataaagagt gatattttta
     6661 gaagaatggg gtgatgcaat gaacattata aataaaattt tttggcaaga agataggatt
     6721 ggcatgatta caaataattt agaggcagat acacatagtc actgtatgtt acaactgttc
     6781 ttagggatag aagacagcat tgaaataaca gttaatgaaa aattagttaa atgtaattgt
     6841 atcatagtag ataaaaatat ctctcattca ttttcagcaa gaaaaaaagt ttattactct
     6901 gctattattg aacctacatc tatatatgca gaacagctta cctcaaaaat gaatgatttt
     6961 ggctactgga tatgtgacaa taatggatta gagaaattac gtcaacaagg aacttttttg
     7021 atagataact ctagtaaaga acaatattta agatttatgg aaatgcttaa taactatttg
     7081 aatataccta taactctaaa acattatgat gacagaatta ctgaattgct caatttatta
     7141 cgtacttgta attgtgataa tcatactatt tcaagttttg cagataaagt gtctctatca
     7201 gcaagtagat tatcacatct gtttaaagaa caaataggga tacctttaaa aagctatatc
     7261 ttatttcatc aaatggagtg tgcttttaga gaattacttt ctggaaaaaa tgttaccgaa
     7321 gcttctatga ctgctggatt cgatactcca tcacactttg cagggacagt taagcgaatg
     7381 atgggaatgc ctgtttcttt atctttgaaa gatagcgagt ttttgaaagt atattaaata
     7441 ttctcgttat ataatgaagt tgtactaaga aaggggcttt gttatgaata aagaatattt
     7501 agaagaaaca aaaatgttaa attataatga accacaactt aagcttttgg tatcatcaaa
     7561 gaattggttg gaattagatg attttcataa aataaaatct atttatgagt ttgtacagaa
     7621 tgatatttta tttggatata atgcttctga tatgttaagc gcaacacagg tattaaatga
     7681 tggtatggga caatgcaata caaaagctac tcttttaatg gcattattac gtgcagtgaa
     7741 tatcccctgt aggcttcatg cttttgatgt aacaaaagat tttcaacgag gagctacatc
     7801 caaattgatt tcactattag caccaaaata tattcttcat acttgggtag aggtatttta
     7861 tcaagatagg tggattgctc tggagggtgt cataactgat aaaaaatatt tagaagcaat
     7921 tcagaagaaa ttttttaatc atggtggaac gtttaaaaag tatgcaatag ctacaaatga
     7981 tttgaaaaat acatctattg attgggatgg taaagatact tttatccaaa aagaagctat
     8041 cgtgtatgat tatggtattt ttccatcacc agacgtattt ttttctactc attcacaaca
     8101 tatgtcaaaa ttaaaaaatt tcatatatgt acatcttatc agaaaaataa tgacaaaaaa
     8161 tgtatgtaag gctagaaaca attatattga taaaaatgaa tgagtgttta agaggtaact
     8221 atataggaaa tatatagaat aattaaaact gtactggtta aaatgaataa aaaggagtta
     8281 tcacagagca aacttaaatt gttcatagga taactccttt tctgtattaa cttctaataa
     8341 tttgatgtct ttttataaaa catatagata caattaaacc agtaaatgca agagtagctg
     8401 ctattgaaaa tgcaacatct attccatgaa tgttagacag catgtttgaa ctcatatgag
     8461 ctttcttagt agcacttgtc attatagtaa taagtatagc agaacctata gctccagata
     8521 tctgacggag tgtgttatta attgctgtgg catgagatat aagttctcta tctaatgttt
     8581 taattcccca tgtagttaat ggcattaaaa ccattgaaat accaatcata cgaatacaat
     8641 acatcaatga cacatgaatt aaagatgtat ctctgcctaa aaaagcgaag gataaagttc
     8701 ctaaaaacaa acatcctgtt cctaatattg atagagcatg aggtccatat ttatctaaat
     8761 gacgtcctgc aattggattg agtacaacca tcaatatagc accaggtaac attaatgaac
     8821 ctgaagataa tgcagaaaat cctcttacag attgaacgta cagaggaatc attaatgtag
     8881 cagacatcat tgaggcatat acaatcataa ttaatattgt agaaataaga aatggtttat
     8941 ttttaaaaac cctcaattct aaaaaaggtt ctttagattt taattgacgc aatgtaaata
     9001 aaattaatga cataattcct attaatattg gtaaatatgt ggcaatatta gtccacccat
     9061 aattaccttg attagtaaat ccaattaata aaccaccaaa acctagagat gatagtacta
     9121 ttgaaggtat atctaatttt agtttttgag tttccccaac atttcttaat aaaataaatg
     9181 atagtataac atctaatata gcaatagggg aaagaaacag aaataaacta tgccaaccaa
     9241 atgaatctac taaccatcct gataaagttg gacctattgc tggagcaaat cctactgtaa
     9301 taccaactaa actcatagct gcaccacgtt tttcctcagg gcataaatag agtataataa
     9361 cttgtaaaag tggcattaga gaaccagaac ccattgcctg caacacacga gcaatcaaca
     9421 tagttgaaaa attattagaa aaaagtgcaa taatacatcc tatagaaaag atagacattg
     9481 atgtaataaa taactttcta gtactaaatc gatttattaa ataagctgtt gtgggtacaa
     9541 taatacctgc aataagtaga taaattgttg ttaaccactg accaacatca gcagtaattt
     9601 taaaatctga cattatatta ggtaatgctg ttgacaatag tgtttgactg agagaactta
     9661 taaaagcacc agtcattaca attgctatta ttaaatttcc ttttttattg ttactttggg
     9721 tttccataat ttataacgtc tcctttttta attgattttt aatgctgttc aataaaagta
     9781 cttttggtct taaagtgtct tctttttcta aatatgcaat cattgcatcc ataattaaag
     9841 aatcatattg ttgatttttt ccctcgacgt ataatttttt tattgcttct agtaatttag
     9901 gattttcaag gagagaagta tccgttttga ttagttcttc tatatcttgt atcgttttaa
     9961 aaaatagcga tttattatga agggaaaagt aaacttcatc ttcttgttta tgtaaaaaca
    10021 ttgatttttc aattaaagga tttagattat ccatattttc tacaactaaa tctagaattt
    10081 gtatagtaaa tttggcaata aagtatacat ttatttctct tttttgtaca tgaataatgt
    10141 atataaactg atgaattaga gcatccaata agaaaactaa atcaaatata attttttcta
    10201 ttttatcacc atagttatca aataagctta gtttgtattc attcaataag ttagctcttg
    10261 attttgtttg caatttactt gtagccttac gttgctcttc agaaaagttt tctcttatat
    10321 aggtatttat ggtagttcta gaaaaaatat ttttccatac agcaattatc tttctgttta
    10381 acttttctga tgatggaatg tcattatttt catttatgga atgtataatt tctaaaaact
    10441 tttcatcata ataattgatt atttcgcaga taaaactttc cttagtttca aaatgcttgt
    10501 aaaaagtagc tttagacatt ttacactctt ttacaacatc cgatattgaa gtattttgaa
    10561 aacctttttg gtcaaaaatt ttttgtgcaa tagttataat gagcctttgt ttttctgtca
    10621 ctttattatc tcctttcacg caaaaacgtt tagtactata tagtacctac aagttttgat
    10681 aaatatatta tacatcattt ggaaaaaaaa tcaatatata gactgtaata tgaaattttt
    10741 gaaaaatttg ttgttatttt tagtcgtata aaaaagaaac ttaagagttt cttaagaatt
    10801 gtttaaagaa gttgcaataa attagtggta tgattatagt atgaaagaga atgtcttgaa
    10861 atttttctgt atcaaattcc aattatggag gtgtagtata tgaaaaagaa aatttgtgta
    10921 gcaataattt ttgctctatt tatagttttt gtagaaatgt ttattgatag ctctagcgaa
    10981 ggaagtaaaa ttatgacagc tgaattaagt aaatacagga aaggcgtaaa tgtattatca
    11041 ataaacaata ttatagtagc gaataaaaaa tatagtttgc ccaaagatta ttctcctcag
    11101 gaaagcagtg aagcgagaga tgctttttat aaaatgaata aagatgctca aaagtcagga
    11161 ttaaatttaa aagcttttag tacatataga agttatgaat atcaagatag attatttaag
    11221 tcatatgtaa aagaacatgg agaaaaagaa gcaaatagat tttcagctaa acctggagag
    11281 agtgagcatc aaactggact tgcttttgat attggaggag atgaccagtc ttgttgggca
    11341 aataaaaagt tcaacaatac aaaagaagct aagtggttgt atgaaaatgc atataaatat
    11401 ggttttatac ttagatatcc agaaggtaaa gagaatataa caggatatat gtatgaatct
    11461 tggcactata gatatgttgg aactgaacat agcaaaaact ttgcaatgaa taatttgact
    11521 ttagaagaat atttacatat aaattaagag atggtttgtt aaaattaggt taaaataaaa
    11581 aataacctag actattagct gtgtaaagtg atataatgta aatacaattt aagataacta
    11641 ctcgaagggg agtagcttac acgaaagtgg taattaatcg tcaagacagt agaaatactc
    11701 ggttaattag caagggattc cttgatagca agaccttcaa aataagcatt aggtatgttt
    11761 gttttggggt ctttttttat tcaaggaatt tttatttaaa aaaatttaaa tttcaagtat
    11821 acataagagc ccccaaacat aaaataaatt tattgaagag gagggcaaca aatgtcttac
    11881 aaaagtactt atcaagaagt aattaaaaat ttagactcaa gtttatctgg tttgagtaaa
    11941 gaaagagcag aagagttgtt agaaaaaaat ggagctaatg aattaaaaga agcagataag
    12001 gttcctactt ataagctttt cctagaaagt tttaaagacc cattagtaat aattctattg
    12061 atagctgctt tagtacaaat atttttaggt gaaacagtgg agtctataat aatttttgca
    12121 gttattatta taaactctgt tctgggtgtt gtacaaacaa aaaaagcaga aagttcatta
    12181 gaaagtttga aaaatttatc tgcaccaaac gcaaaggtaa taagagacaa taaaaaaatg
    12241 acaattccag caaaaaattt agtagttgga gatattgtat tcttagaagc tggcgattac
    12301 ataccagcag atggaaggct tatagaagca caatctctca aggttgtaga agggatgtta
    12361 actggagaat cagaaccagt tttaaagcat acagaaaaaa taagtgaaga tgtaagttta
    12421 ggtgaccaga aaaatatggt atttagtggt gctgtagttg tttatggtag aggttctttt
    12481 gtagtgactg gaacaggtat ggatactgag atgggtaaaa tagctgggct tttagaaagt
    12541 gctgaaaata aacttacacc attgcaacaa aaaatagagg attttagtaa aaaacttggt
    12601 gtaggtattg taatacttgc tcttttaata tttataattc aagtagcaag aaattacttt
    12661 atggctggaa atggagaaat gacacaaatt attttagatt cattcatgtt ctctgtagca
    12721 gtagcagtag cagctatacc agaagctcta tcatcaatag tcactatagt attggctgtt
    12781 ggaacaaata gtatggcaaa aaaacaagcc ataataagaa aattaccagc agtagaaact
    12841 ttaggttcaa caagtgtaat atgtactgat aaaacaggta ctcttacaca aaataaaatg
    12901 acagtagttg attattatat gtatggaact aataatgatg agttagataa agaaaaagta
    12961 aactattctt atcatgctaa attattgact atggtttcta cattgtgtaa tgactctcat
    13021 ataactagtg atggtaaaga agttggtgac ccaacagaag tggctttaat aaattattca
    13081 agtaaaaatg atttagatta tgataaatta agaagtaagt atatcagaat aaatgaaata
    13141 ccatttgact cagacagaaa gcttatgtct actgtaaata gtatatatgg agattatata
    13201 atgtttacaa aaggtgctcc agatattgtg tttagtagat gtaaatatgc tttgaaaaat
    13261 ggttcaaaag tagatataac agatgagata atagaagaat ataaacataa aaatgaagag
    13321 ttctcaaata gagcacttag agtattagct tttgcaataa aagatgtccc agaagaagat
    13381 tttatacctt caattgatga cgaacatgac atgacattgg ttggaataat ggcaatgata
    13441 gacccaccaa gagaagaagt aatggatgca gtaaaagagg caaagagtgc tggtataaaa
    13501 actgttatga taactggtga ccataaaaca acagcagcag caattgcaaa agagattggt
    13561 ataatggaag aaggagactt ggctcttact ggaaaagagc ttgatgcttt gagtgaagaa
    13621 caactatatg aaaaactaga aaatataagt gtatatgcca gagtctcgcc agaaaataaa
    13681 attcgtatag taaaagcatg gcaacataag aataatataa cagcaatgac tggtgatggt
    13741 gttaatgatg cacctgcatt aaaacaagca gatataggaa tcggtatggg aagtggtact
    13801 gatgtagcaa aagatgcttc tgctatggta ttggttgatg ataactttgc aagtatagta
    13861 aatgctgttg aagttggtag aacagtatac agtaatataa taaaatcaat tacttatcta
    13921 tttgcaggaa acttaggagc tatagtatcc atagtatttg cagtgtttgc aggttggtca
    13981 aatccattta cagctttaca acttttgttt ataaacttag taaatgactc tttaccagca
    14041 attgcattag ggtttgaaaa gtcagaaaaa aatataatga ataaacctcc tagacacccg
    14101 gatgaaagta tactagcagg aggaacattg aaggcagttg ctataagagg ttgtataata
    14161 ggtgtagtta caataattgc acaatatata ggattacaag tatctccaga acttggagta
    14221 gcaatggcat tttcaacatt gatattagct agaataatgc aaacattacc tgcacgttct
    14281 aataatcaaa cagtaataaa attaggtttt ttcagcaata aatacgttat aggagcagtt
    14341 ttagtctgtc ttgggcttta ctctttaaca ctaataccag tgctaagagg aatattctct
    14401 ataccaacaa cgtttggatt gtaccagttg gggatttgcc tagggttagc attaatttca
    14461 tcaatattaa tggaacttaa caaattaata aatagaagtg aagtaaaata atcaaaaaat
    14521 taaagggcat attgatttta acaatatgcc ttttataaat agtctaattt attctatatt
    14581 atcgattatt tggaatgctt ttataacttc aggtaattct ttttcttcag gcattctaaa
    14641 tcttatgaaa tttttatcaa gtccagtaaa atcacttcca gatataactc ttatttttaa
    14701 tttagcaaac tcttgttgta aatctatgtc cttgttttta tgttcaactg tcataattga
    14761 tacagtatca ctagtttcag ctatattaag gtttttccat ggaattaata attgattttt
    14821 tatatctttt gttttttctt ttaattcttc tataaattga acatcatcta ataaatttgc
    14881 tgcaatacta cgagatattt cagacacttc gtaaggagta gaaatctttt taatagggct
    14941 aacaagttgt tctggaagaa ctgcatatcc tgctcttagt cctgctaaac caaatccttt
    15001 tgaaaatgtt tttaaagcaa ttacattatc ataattattt agaagcttga cagctgaatt
    15061 ttcttttggc atgtattcac cataagcttc atctaccata actgctatat catatttagc
    15121 tgcttccttt actatatttt caattgatga taaaggtata atttgtcctg taggattgtt
    15181 tggattgtct atgtaaatta ctttatattc tggatttatt ttttcaataa attctttttc
    15241 attaaacttg aaattatcct ctttctttaa aagaacataa tcataagttg caccatgcat
    15301 ttttatatct gtctcatatt ctgagaactg aggtgaatat cctaaaactt tatctccctt
    15361 ttcaataaat agtctattta aaaggtaaat aacatgtata gaaccatctg ccaaacaaat
    15421 tctgtcagta tctaatgcta taaaatcttt ccaataatca actatattat cttttaaagc
    15481 aattgaatga ggatatcctc ttatcatatc aaattttaaa gcattaaaag cttctacagc
    15541 tttgtttgag taagacacgg tatttatacc ttcaccacag tctatctcga atacatcttc
    15601 tgaatgtcca gattctattg cataactttt tttcaactgc atttcaatag ctttgttaat
    15661 ttttagtttc atactttatc actccttatt tagtcaataa taaatgttat aaaagttgct
    15721 agatttaaaa tattatgaaa atatataata gtaaaaaatg aatataaata ttataatagc
    15781 tcactttatt taatatgatt atatatcata tattgtaaaa tttctagcat tataagacta
    15841 taaattgaaa atattcagaa aaatactaca agaaacgatt tattttgctc ttaaaataag
    15901 ttaaatgaat gttattaatt ttgtaaattg aaatagttta agtataaagt tatgatataa
    15961 tctaataagg gtaattcttt agattatatt aaggaaatat aggtatacaa taaaaagtta
    16021 tggagttgga ttatgggata taaattaatt gtaacagata tggatggaac tttattaggc
    16081 aataatcata aagttacaga tgaaaataaa actgcactac aaaaagttat aaaaagcggt
    16141 attaatgtaa ctttagcaac aggaagagcc ttcgattcag ctaaatgcaa tgtagatttt
    16201 cttaaagagg acatgccaat aatagcttgt aatggttcat taataagaga gcaaaatgga
    16261 aatataatat attcaaataa gattgataca agaacatgtt taaacatttt agatgtatta
    16321 gataaatacg atatttacta ccaatgtaat agtatagatt ctatgctttc gaaaaaaata
    16381 gaaggacgtg aagatagatt aagtgtattt ttaggctctg aaacagaagt aattgtaaaa
    16441 gatgatttga gagaagaaat ctttaaaaaa gatatactaa aatttgtaat catagaagaa
    16501 aaaaatccat ctattttgga tgaaataaga aaagaattga gaaaagttca aggaataaag
    16561 ataacttctt catggccaaa caatattgaa gtcatgaatg aaggtgttga taaaggtaat
    16621 gcagttaaaa ttttagctga aaaaatgaat atagacagag aagatataat tgcatttgga
    16681 gacaattaca atgacataga aatgataaag tttgctggtc ttggggtagc tatgggaaat
    16741 gcagaagaat tgattaaaca agaggctgat tatgttacag atacaaatca agacagtggg
    16801 gttgcaaagg caatttataa attcctagag ttaaaagaat cacttagctc ttaaaacagt
    16861 atataatagg aaaaaatagg ttgactattt tgtagatagt aattataata caaatagata
    16921 aaacataaaa atttagattc agtgaaaaag agagtagata tagagaaaat ctcagcgaat
    16981 tgggggatgg tgtgagccta atgataacac tttatattga aaagaacttt ggagaatact
    17041 acttgaaatt cagtatggta gtaggtaatc tcgccttaag agaatagagt gggtaaacat
    17101 tgtttaccaa ctagagtggt aacacggaat atttcgtctc ttggtctttt tgaccaagag
    17161 attttttagt ttcattgagt tacaagactt ataattaaaa tacaaaaaat taaaatatga
    17221 ggtgaaagtt atgaaattat ggggcggacg ctttagaaaa gcggaaaatc aacttatgga
    17281 agaatttaat aaatcttttg gctatgattg tgtactgtac aaaaaagata tcgaaggaag
    17341 tgtagcacat gttcatatgc aagtaaaatg tggactttta acagaagaag aaggaaagtc
    17401 tataacagaa ggtttaaaag ggatacttga agatgtagaa aatggaaagt tagtattgga
    17461 tggagagtat gaagatatac atagttttac tgagataaat ttaattcaaa gaattggaga
    17521 tgttggcaag aagcttcata cagcacgtag tagaaatgac caagttgcag ttgatatgag
    17581 attgtatgct aaagaaaaag caaatgattt ggtggattta atatctgaat ttaaagctac
    17641 tataaaagat gttgctgaca aaaacccagt gatgatgcca ggctatactc atcttcaaag
    17701 ggcacaagta gtgacattta agcatcactt aatggcatat tatagtatgt ttgatagaga
    17761 tgagaaaaga ataaaaaatg ctatagaaat attagatgaa tcacctttag gctgtggagc
    17821 tttagcagga actacgcatg atatagatag aagtataact tgtgaacaat taggatttaa
    17881 aaaagtagta gataatttta tggatggagt aagtgataga gattatttac tagagctaat
    17941 gagtgatttt tcaattataa tgatgcactt aagtagatta agtgaagagt tgatactttg
    18001 gagttctcaa gaatttggat ttgttgagat agatgattta tatacaactg gaagtagtat
    18061 aatgcctcag aagaaaaatc cagatggtgc agagcttata agaggtaaga ctggtagagt
    18121 atatggaaac ttatttggtc tttttacagt aatgaaagga ataccattag catataataa
    18181 agacatgcaa gaagataaag aaggtttttt cgatagtgta catactcttg aaatgtgtat
    18241 acaaataatg gatagaatga tagccacact aaaggtaaat gaggataaaa tgaaacaagc
    18301 agtaaaaaat ggttttttaa atgcaacaga agttgcggat tatttggtta aaaataatgt
    18361 tgcttttaga gatgcacatg gtatagtagg aagtatcgtt atatattgtg aagataataa
    18421 aaaagcaata gaagatttaa cattagaaga attacataag ttttcagatg cttttaagga
    18481 agatatttat gattttatcg actatgagag tatattaaat aagggaataa aaaagaattt
    18541 aaaataatgt atttattttt aaaagctgta aatttgaatg ttatcattta aatttacagc
    18601 tttttattgt gtaagatttt attatataaa agtaagaaac ttaaaaggag tagtatttaa
    18661 aataaataca aataaaaaat tattattaaa atgataatta atatcaataa gtattgacaa
    18721 agttatttct aaatactatt atatataata taaataataa tgaaaatcaa tatcaaataa
    18781 atgggaggaa atctaatgaa cttattagtg attggtggtc atgaaagaat ggaaaaagat
    18841 tatatggtta tggccaagaa aaaaggttat aagacaaagg tatatactac catgtcatct
    18901 aaattaaata attctatagg aaaaccagat gcagttgtca tacttacatc aactgtttca
    18961 cataagatgt ctagaacggt tgagtctcaa gctaaaaaac aagatatatt aatcgtaagg
    19021 cataaaaata gtagtaaggt agcatttaat gaatgtctag atatggttga tgaatgtctg
    19081 ggaaattgtt caaaatgtaa gcttaaagac aaacaaaaac aaaaaaatat ataggtataa
    19141 aaaatacata aacataaaat taaaagtact gagataatag ttataattat tatctcagta
    19201 cttttagatt tataaggata agtatagtat aaatttttta ttataatttc caactacttg
    19261 ttactttttc atattttttt tacttctagc tttttttctt tttttcttct ttatatttga
    19321 agctattata aataaaacta aaagcactag aatacctgca ataatcttaa caaaagtcat
    19381 taaaacatta ttttcagtca aaaatggaag actgctatca agattatttt ttgcaattaa
    19441 gtctatactg ttttctagtt taccattatt atatacattt aaagtaccaa ccttagctcc
    19501 tttttttata ggcaaatcaa ttttatttaa ctctgttttg gtatcataat tgccttgaga
    19561 ttcatctttt tctaagaata tcctataact attttttggt tcataaatca attcatgttg
    19621 ttttgtaaac aatacttttt tagtatctgt gtatttttct ttatcaacaa tagtagcaca
    19681 cttaaagtta tcaaatccat aatcaatcag aatacgtgag tctaaatata aatcagcatt
    19741 agttgaatta aatacagcaa cgataactct tctgccatct tttacagcac tcgataaaag
    19801 acatttacca gcagcatcag tatatccagt ttttatacca tcaactatat catattttat
    19861 atttataggt tgacctttgt atgtaatctt atcatgtgat gtcaaaaact tattagtgtt
    19921 tacaaagtat ctctcataag gatatgcttt tgtagcttca aatttaagtg acttagtttt
    19981 tacaatttgt ctaaaagtat cattattcat agcttctcta gctatgagag ccatatcatg
    20041 agctgttgtg tagtgatttg ggtctggtaa tccatgagga ttattaaaat gagtattagt
    20101 agctccaatt ttttttgcct cagagttcat gaggtcaata aatttttgta cattgccacc
    20161 accaacatat ctagctaata caaaagcagc atcattagca gaatgaacca aaagagcatc
    20221 tagtaactgc tttacagtaa aagattcccc attttttaga tacatactag agccatctat
    20281 ttgaggtaaa tctttaattt ctattacttg atttaaatct ttaacctcct ttagaactaa
    20341 acaagctgtc catactttag ttgttgaagc tggataaagc ttagaatttc catccttatt
    20401 atatagtatt ttgccagact catagtccat taaaactgca tgtttagcaa ctatagctgg
    20461 ttcattatct gcaaaagaga tattagagct tcctaaaaat gaagtaaata tcaataaaca
    20521 gattaataaa agtgataaat ttcttttcat tgtgagcctc caaattttaa attcatcttt
    20581 tagtatagca tatatttgtc aataattaaa agaaaggagt gattaatata tgaaaaaaaa
    20641 ggtaaaattt atttcgtttt ttgttttatt tattttaata atatcatcaa taatcgttta
    20701 taaatcgctt attttcaaag atgatattta tgttgtaagt aaaaaaaatt tacctaatga
    20761 aaaaacacat gttgtagaga atgaaattaa aggtgaagat aataatcaca gtaaagtaac
    20821 taaagacata gataaaaatt ttcaaaataa taaaataact atatacataa gtggagcagt
    20881 aaatagacct ggaattgtaa ctatagagtc agacaagaga ctttatgatg ctgtagaatt
    20941 actaggagga acgacaaaag aagcagattt aaatggagtc aatctatcag taagattaga
    21001 agatgaacaa cattacataa tacctaaaat aggtgaagca acatctgtta caagtaatga
    21061 tgattccaat aaactaaatc agaagaatga gtcaagaggt gagcctaaaa ataaatccaa
    21121 taaaaatata tctaacgaat caaaagtaaa tataaatgta gcgacgatag aagagttgga
    21181 ttctctacct ggggttggag aagctacagc caataaaatt ttacaacaca gagaagaaaa
    21241 tggacagttc agttcaattg aggaaataaa aaatgtaaat ggtattggag ataaaaagta
    21301 tgaaaatata aaagatttaa tttgtgtaga ctaaaatata atacaaaact tacatataga
    21361 tatgttatta taaatatgta tgtatatgtg tgtacatgtg gaaatttagt aaaggaggca
    21421 cttttatgaa aaaagaacaa aaaaaactta cagagatgac aacagcaggt ggttgagccg
    21481 ctaaaatagg gccagaggtt ctggcatcag ttttatctca attgcctaaa aatgataata
    21541 tagaaaattt attagtagga ctagatacag cagatgatgc tgcagtttat aaattaaatg
    21601 atgatatggc acttatccaa actttagact tctttacacc aatggtagat gacccatacg
    21661 tatttggtca aattgctgca tcaaattcac tatcagatgt atatgctatg ggaggcaaac
    21721 ctttggttgc tatgaatatt gtttgttttc catcatgcca tgatatggat gtacttgcag
    21781 aaatattaaa aggtgggttt gataaagtga aagaaagtgg agcacttttg gttggagggc
    21841 atactgtaga tgacaaagag cctaaatatg gtctttcagt ttctgggatt gtaagtccaa
    21901 ataaagtttt atcaaatgct actgcaaaac ctggtgataa gttaattata acaaaaccaa
    21961 ttggagtggg tgtattaaat acagccatga aagaaggtat ggtagaacaa catatagcag
    22021 ataaagtaat agaaataatg atacatttaa ataaatatgc agcaatgagt tttgagaaat
    22081 ttgatgtaaa ttcggttaca gatataactg gttttggtct acttggtcat actttagaaa
    22141 tggccaaagc ttctgaagta agtattgaaa ttgaatcaaa acatgtacca attattgaag
    22201 gtgcaataga aatggcacaa atgggaatta tacctgctgg tatgtataaa aatatgcatt
    22261 atgtatctaa agatgtagaa gttgtaggaa atatagaagt tgcagttcaa gatattttat
    22321 acgacccaca aacatcagga gggttgttaa tttcagtaaa agaagaactt gcagaggaac
    22381 ttgtaaaaga tatgaaatta aatggagcaa tagaagctaa aattataggt tctgtagttc
    22441 caaaagggga aaaatatatt acagttttat aaaatattat agagaaagag tgattgtttt
    22501 gagcaaaaga gaattgtttg caatgttgcc ttcagttgat gaagtattaa gtgataatag
    22561 aattgtagaa ataataaatg aatatcctag aagtttagtt ttagaatctg tgagagaagt
    22621 aatagattta aaaagacaat ttatattaag attaaaagaa gatgcatcaa actctgtaac
    22681 aatagagttt ggtgaaataa tagaatctgc aatagagaga gttaagttaa attactcatt
    22741 atctttaaaa aaagttataa atgcaactgg tacagttatt catacaaatt taggaaggtc
    22801 tttacttagt gaagatataa aagatgaatt atggtgtgct gcttcaaggt attcaaactt
    22861 agaatacgat ttggataatg gagaaagagg ttcaagatac tctcatttaa ctagtacaat
    22921 aaaaagactt actggagcag aagatgtact agttgtaaat aataatgcag cggcagtact
    22981 tcttgtactt agcacaatgg ctaaaggagg agaagcaatt gtatctcgtg gagaattagt
    23041 tgaagtaggt ggttcattta ggattccaag tataatggct cttagtggtg cagaattagt
    23101 tgaagttggt tcaactaata aaactcattt gaaagattac aaggaggcca ttacagaaga
    23161 tacaaatgta ttgatgaagg ttcatacaag taattataga ataatgggat tcacagagag
    23221 tgtttctata gaagaattgg ttaatttagg taaaaaatat aaactacctg taatagaaga
    23281 cttaggaagt ggagttttta tagatttatc aaaatatggt ttatcttatg aaccaactgt
    23341 attggattct attcgacaag gtgcagatgt agtaactttt agtggagata agatgcttgg
    23401 aggtccgcaa gcaggtatta tagttgggaa aaaagagtat atagaaaaaa tgaagaaaaa
    23461 tcaacttact agagcactta gagttgataa gcttacaata tgtgctcttg aagcaacatt
    23521 gagaatgtat ttagatgaga caaaagctat agaaaatata cctactttaa aaatgttgac
    23581 atataaaatt gaagaattag aagtaaaagc aaataaacta tttgaaaaaa taactgcttt
    23641 aaacttaaat gcaaacataa atatagaaga tggattttca caggtgggag gagggtctat
    23701 gcctcttgaa actataagta caaaggtaat atcaataact cctgaacata tgaatgtttc
    23761 atctttagaa aaaaaactaa gacttagtga agcacatata atagccagag tgtatgataa
    23821 taaatatgta ctagatgtaa gaactatatt tgatgatgaa tttgatgtaa tagttgagga
    23881 attgagaaaa gcatttaatt aaataaattt gagttaataa atattaaatt tagaagtata
    23941 agtatgaagt tttgattaaa aaatagctac acacacttgt gactttagtc atagcaggtt
    24001 tagagggggc aaaaaatgaa aaatgtagta attggaacag caggtcatat tgaccacgga
    24061 aaaactactc ttataaaagc attgactgga agagagactg atacgttaga tgaagaaaaa
    24121 aagagaggta tttcaataaa cttaggattt acttattttg atttaccaaa caatagaaga
    24181 gctggaattg ttgatgtccc aggacacgaa aaatttataa aaaacatgtt agctggagca
    24241 tcaggaattg atatagtttt acttgttgta gcagcagatg aaggggttat gccacagaca
    24301 gttgagcatt tagatattct aagcttttta aatatcaaaa atggaatagt ggttctaact
    24361 aagtctgata ctgtagatga tgaatttaga gagctagtaa aagaagatat ttgtgaaaaa
    24421 ataaaaggaa cttttcttga agatgctgaa ataattgaag ttgattctat atctaaaaga
    24481 ggtatagaca ctcttattga aaaaatagat aaaatgagtg atgatataga agataaaaat
    24541 gaaaactctc cagcaagact taatatagac agagtatttt caataaaagg gtttggtact
    24601 gtaataacag gcactttaat agaaggaaag ataagtatag aagatgattt ggttatatat
    24661 ccacaggtat taaaaacaaa aataagaagt atacaagttc atggagaaaa taaagataca
    24721 gcttatgcag gtcaaagaac agcaatcaat atatcaaatg taaaagtgga tgatataaaa
    24781 agaggagatg ttttagctgc accaaactca ttggaggaat caatgatgtt agatgtaaaa
    24841 ttatcattgg taaatcattt agataaagga ttaaagcact gggatagatt aagattgtat
    24901 catggaacta gagagatact ttgtagagca gttccactag ataaagaatt aattgaaagt
    24961 ggagaaagtg gctatgttca gttgagactt gaagaaagca tagtatctaa gaaaggtgat
    25021 acgtttgtag tgagacgtta ttctccaatg gaaactatag gtggaggtgt aataattgac
    25081 ccatctccta aaaaacataa gaaatttgat gaaaaagtta tagaagcatt aaaaataaaa
    25141 gaaaaaggcg aactaaaaga tataatagaa gagtacttaa aaaggaattt aaaaaattat
    25201 ccaaatataa aagaaataat gagctatagt ggtgctcatg aagaagatgt aaaaagagcg
    25261 cttgaaacac ttatatcaga agataaagta tttattatag gaaatatgta tatgcatata
    25321 aatcaataca ataaattaaa agaaaataca ataaagcttt taagtgagta ccataagaag
    25381 tataggctta gaaaagggat actaaaagaa gaagtaaggt ctaagataga gagtaatttt
    25441 aagactaggg agatggatat tttattagaa aaattgtcta cagaaaatgc tataaaaata
    25501 gaaaataata tagtatctct attagatttt gaggttattt taaatgataa gcaaaaagaa
    25561 atagcaaaaa aaatagaaaa aagattaaaa tcatgtggag tttcttctat actgacaata
    25621 gatgaagttt ctgaaggaaa tcataactat gctgaagtat tggagtcaat gataggtaat
    25681 aaagtagaga aattagatga tttgtatata atggacaaag atatttatga aaatgcaaag
    25741 aacattttga taaattatat aaaagaaaat aaagaaataa cattaggaga atacagagat
    25801 ttgattgatt ctagcagaaa gaattgcatg attatattgg aaaacttcga tagaaacaaa
    25861 ataactaaaa gagtagaaaa caaaagaatt ttgttttagc agaaaatgtc tgtttttgct
    25921 ttaaggtata aagaaggaat tataatgtcg agtatcgtat aattaattag tataaaatat
    25981 taattaagag gaagtgtaaa gtattgaaga atataatacc tgatgtaaaa ccagatataa
    26041 aagaaagaga agacatgagc cttttattta taataatggg ctcagtactt ttattattta
    26101 ttataaaaga tatagagaaa tatgaaactt tattacatgc catatatacg atatttttag
    26161 tagctacagg agtaattata tttaatacta caaaatttaa agtagattca ttttctacat
    26221 ttttaggatt aatttttata gctactggag tattggagtg tatatattta tttaattcct
    26281 tggggattaa aactaagtct ataatggaga ttaacatcac aatatcagca ataacagatt
    26341 tatttcctat actgggtgta tacttaagtt ttaaatttgt taaggataac aaacaaattt
    26401 attcaagtgt aattttattt atagcaactg ctacattaac aataacatct ttattcataa
    26461 tatcaggaat ttgtgactat ctgggaaatg gtaaatcaca atatgcttta ggagttataa
    26521 tatctatatt cataataatt atttctatca ttagtggaat tgaattaaat tcaagttcaa
    26581 ataaaggtaa atgggaatat gatgaaaaaa agatttttaa cagaataata gtaataataa
    26641 tcttatcaag agttccaaat ctattgcata tagttattga taatagaaat gtagagagga
    26701 tattatcaca aataataata aatgtagcct tatattattt atataattac atagtatcta
    26761 agaatattaa gaaaacagca ctggaattgc atgatacgaa tgaagaatta acaaaaaaaa
    26821 cggaaagttt aaaagaaaag aataaaaaat taatctatga gacagaaaag atagaggaac
    26881 ttaagggaat acttatgaaa agagaatcta ggctacaatc tacattagat gtagcagtaa
    26941 atagcatagt agtatttagc aaagatggag aaattacata tgcaaataag agctttagaa
    27001 atacttttgg agactataaa gaacaagata aattgactga taaaataaaa aacttcaatg
    27061 atttgataga aagcattcat tatgtgtttg ttcatgagaa gaatgcagag aaacttatta
    27121 gtacaagcga taataaagtt tatcaagcta tatttgcacc tttaattatt gcttcacaaa
    27181 cagaaggagt attgtgtgta ctagaagata agactaagaa aaaggagtac gaacgcaaac
    27241 ttattgaagc aaataagaga agtaaagatt ttttagaaag tattggagat ggcatagtag
    27301 tgcttgaagg taataacaaa atatatgtta ataatgcatg tagggaaatt tttaaaaata
    27361 atttagagag tattgatttt tcattagtat gtaaaagtga agaaagtata gaaaaaagat
    27421 atgtaataga tggtgaagta aagtatgtag agatgagttt ttctcagtat actaatgctg
    27481 gtactaataa aactattatt gtaatacgtg atacaacctc tcgaaaaata tctcaaataa
    27541 aacttgagga aagtcaatca tcatattcaa gatttataga tatattacct gatggcatat
    27601 gcttattaaa agaagattta agtataaact atgcaaataa gtcgttttta gatatgctag
    27661 cttttacaaa catagatgat ataaaagatt ctaatattaa actattaatg aatgcgaata
    27721 ctgaagaaaa aatgaaattt acggataata tggctaaagt tttaaatgaa aatagaagta
    27781 tacttttact ggaatatgaa ttgataaata gttatgatga tatagtagaa gtagaattaa
    27841 gtgcattacc ttttgctata tacaatacta gatacataat gcttattata aaagatttag
    27901 tacataagaa atattccgag caagcagaaa aagaactgct tgaaagattt aaaacagata
    27961 aaatcaaaac ggagtttttt gcaaatatgt ctcatgaact aaaaactcca ctaaatgtta
    28021 tatctagttc aaatcaactg gtagattctt tctatagaaa tgaaaagata aaagattaca
    28081 ataacaatat taaatctcat gtggatttag ttagacaaag ttcttatagg cttcaaagat
    28141 taataaataa cattatagat ttaacaaaga tggagtctgg gttctataca ctaaaacttg
    28201 cgaaatacaa tattgtatct gtaatagaag acttatttat gaatatagaa gagtatgcac
    28261 ttagaaaaga cataaaaatt ctttttgata cagaccttga agaaattaat gtttatatag
    28321 acaaggttga aattgagaga attatgttga atctattgtc aaactgtatt aagtttactg
    28381 ataatggagg ctggatatat gtaagtattc attataaaat agataaagta atcataagtg
    28441 taaaagacac tggagttgga ataccacaag ataaattaga gttgattttt gaagaatttt
    28501 cacaagttga taagacttta tccagaaata ctgaaggaag tggaataggg cttgctatag
    28561 ttaagaatct agtaagtctt catggtggag atatagaagt agtaagtgaa gtgaataaag
    28621 gcacagagtt cttaatttca cttccaatga agggatttag tagtgaacat tacacagaag
    28681 ataagagaat atataatatt caagaaaaaa taaaaataga attttctgat atatattatt
    28741 aaaaatgatt atgagcaaaa ataaaataaa aatactattt gtttaaatat ttaagttgta
    28801 agaaataggc tatcataagg taaattatta tgatagccta tttttagcta ggttttatat
    28861 ctatatgtga gaacatataa tctctaaatt accttcaaaa ctaaaatctc caaagtcgtt
    28921 tggcagtata aaatgcatac ccttttttaa attatattct acagaattgt gtataagttt
    28981 tccatttcca tccaacacac tgtataaaga aaaaggtgta ttgtgtgtaa aattacattt
    29041 tccaaaaaca tctaatttat aaacactaaa aaattcgttt gacacaaatg ttgtgcattt
    29101 aaagtctgat gttgatacga ttttatagtc agtatcgaaa tttatatgag gaacattcgt
    29161 aacatctatt gatttttgaa catgaagttc acgcttatta cctgaattgt cagttctatc
    29221 ataatcatag actctataag tagtatctga gttttgttga gtttctaaaa tcaatgttcc
    29281 tttgcatata gcatgtatag tgccacttgg aacatagaaa aagtctccct tttttatatt
    29341 aacctttcta agtagtttgt cccattcatt attatttacc atatttacaa attctttatg
    29401 agattttgca ttatgaccta ttataatttc tgcatcatca gaacaatcta ttacatacca
    29461 acactctgtc ttacctaact ctccattttc atttttttta gcatattcat catttggatg
    29521 aacttgaact gataaattat catttgcatc caatattttt gtaagtaaag gaaacttatc
    29581 accaggtgtg tttccaaata attctctatt tttattccac aattcagata gttttttacc
    29641 tttatatttt ccattttcta ttaagcaatc tccattttta tgagagctta tagcccaaca
    29701 ttcaccagtt gttggcgaat ctatttcata attaaattta tctttaagtg cagtaccacc
    29761 ccatattcta tccataaaaa taggttttaa aaataatggt tccataatcc tgttcctcct
    29821 atgatattaa atttatccta caaataatta tatataaata gattataaaa tcaatagtaa
    29881 aaaagttttg tcccttgaaa tgacataaag tataaggagt tattaaataa gataaactat
    29941 gatatataat ataaagttct aaattggagt gataattatg gagttgttta aggcagaaag
    30001 caaaaaatat aaaaaagaat ttttaaaatt attggagcaa tgggttagta taccttcatt
    30061 ttatgataga aaaactgtat ctaaagacat gccttttgga aaaggagtat atgatgcatt
    30121 aaactggttt gaaaatctag gtagagaaaa caatttcaaa gtaaaaaata tagataatca
    30181 tgctgttcaa attgaatatg gaaatggtaa agagtatgta gatatatttg gacattgtga
    30241 tgttgtaaat ccaggtgaag gatgggatag tgaaccattt aaactaaata ttataggtga
    30301 taaattagtt gctagaggtg tatctgataa taaagggcct atgatagtga attttttagc
    30361 actaaaaatg ataaaggatt tagatattaa tttaaaaaga aaggttagat taatagctgg
    30421 gggaaatgaa gagagtggat ttaaatgtat aaagcattat tatagcaaag agccatatgg
    30481 tgtatgtgga tttactccag atgctaaatt tcctgtatta aatggagaaa aaggtggagc
    30541 aataataaag ttaatttcaa atatagacga taaatcttta tatataagtg gtggaataga
    30601 gtttaataca ataccagata aagtttatat aaagaatgta gaaaaattgg gtaaagataa
    30661 tatatgtttt gatattaaca atatatcgat aaattatgat aatggcaatt atattgtgta
    30721 tggaaaaggt ggtcattcat ctaaacccga aaaatcaata aatcctatat tagctacaat
    30781 taaacttttg tcagaaaata ttgatgaaaa atggacaaaa gatttatata agttgataaa
    30841 tcaagataat ataaatggaa acttatttgg tttaaatatt gaggggaaat gtggaatttt
    30901 atcaatggtg ccaacaataa taaatatagt agatggaaaa ttagaagtag ttttaagcgt
    30961 tagatatcca gaaatattaa ccattgaaga tataataaaa aaatttaatt tgtatatgga
    31021 gcaaaataat attaataaat ttgagcttat tggggaaaat ttaaagcaag caaattatat
    31081 agatagaaat tcaaaacttg taagaagttt gcatgatata tatataaagt attctggaga
    31141 tttaaaaaat gatgttagag taactagtgc aggaagttat gcttctgaaa tgaataattc
    31201 agtaatattt ggttgtgaat ttcctgatgg tagctttggc aatgtccata gtgcgaatga
    31261 atttgctagc ttagatagat ttataactgc aataggtata tatgcagagg caataattac
    31321 attatgtaat aaaatataaa tacaaaatgt cccttatata gacatattat atatacaatt
    31381 aaaattttac ttgatatata attgaaatca aagagaaggt gatacaatga taactaagaa
    31441 acatgcaata atagtaaaga gtttaagcaa taaagatgga tatatgacat caaacgaatt
    31501 agctatcaaa ttggatgtgt ctactaaaac tattaaaaga tatatagcag atttaaatag
    31561 tgttttaagc aaatatgatt tggtaatagc ttcttctaga ggtataggat acaagttaag
    31621 cggttctaaa aacaatatag ccagagctgt taaagaagca aataaatata ttgatggttt
    31681 tttagatgac tctgaagagt caagaatgtc aaatataata tgtatgctta ttaatagaaa
    31741 ttatatgagt atagaagcaa tggcagaaga attaaatcta agtatagccg ctataaataa
    31801 attgtctagt aagcttaaaa gaaaacttga aaagtatgac ttagttataa aatctaaacc
    31861 ttactatgga agctatatac atggtgaaga aattaatata aggcaattaa ttactgacta
    31921 tgcaataaaa ttagatgaaa aaagtaaagt gaaggtgttt ttagatgaca ttagtgaaaa
    31981 cgatatacac tgtatagaaa gtattttaga aaagcactta agagaaaaag atacaataat
    32041 ttcagataaa gattttaatc ttttgctttc aaaaataatc gtttctgtat ttaggagtaa
    32101 gagaggtcat agtaacaata taaacttaat ggatacaagt tatagatttc ataactacta
    32161 ctttatagaa aatctaatga aagaaatatc tgataaaatt ggatttaaac ttattgaaga
    32221 tgaagtaatt tatatttcaa attattctgg tgtaatagcc tataaaggta cacaaggaag
    32281 aaaaaacaca agtgaaattg aggaacgaat aagtactgtt ataagtagtg cacttcaaga
    32341 tatattcctt atttcaggta gtgattatac aaaagatgat gagtttatgg ttgcaatctc
    32401 tgaccatata aagagatttt taaatagagc tagagctaat gtaaaatcaa ataatccttt
    32461 acttcatcaa ataaaagaga agtttcctat agcttttaat ttagccgtgt ttttatcaaa
    32521 taaactagaa actgagttta atttaaaact tgatgaagat gagcttggat atatagctat
    32581 ccactttgct gcttcaaatg aaagaatgaa aaaaaatact agtaagaaaa tttgtatagt
    32641 ttgtcattat gggataggaa caggacaact tatttctgaa aagctaaaac agaatataag
    32701 tgatttaagt gttgtgggtg tttatccagt tagatatctc gatatggcta taagtcaaga
    32761 tgttgatttg atagtatcta cagttgaatt aaaaggatat gaaaaacctg tcttatatat
    32821 agaaaatata tttgatgata gcttaattga aaacgtaaac aaagcctttt atgaaaagga
    32881 agaaagaaga aagataataa gtaacatgtt tgatgaaaaa gcttttttca gtataaaggc
    32941 tagtactaaa gaagaggtca taatttcact ttcaaataaa ctaaaagaaa gagattttat
    33001 agaggaaagt tctataaaaa gtataataga tagagaaaat atttcatcaa ctgaaatagg
    33061 aaatctagta gcaatacccc acacaatagt gaagggagat aaaaaatcta taataggagt
    33121 tggaatactt gaaaatccaa taatttggga taagcaagag gttcagctag tatttatggt
    33181 attttttaat actaaagaaa aacataattt ctcgatattt aaataccttt ataattttat
    33241 aaaagatgaa ggaggagtaa gaggaattat taaaatatgt gactttaata aattaatggc
    33301 acttataggt aattaaggag gaaattaata atgggaacaa acatatttaa caaagaatat
    33361 gtatttttaa atgttgatgc taagtcaaag gttgaagttt taaaatttat atcaaaaaaa
    33421 gctaaagact taaacttggc agaagatgag agcttagtat atgaaggttt aatggctaga
    33481 gaagaacaat ttactacaaa tttaggagaa tctatagcaa tacctcacac taaaaatgat
    33541 gcaattgaaa atccagcagt agtagtattg aagtttaatg aagatgttgt ttggaatgaa
    33601 ggtgaggata aggttaaatt agcaataagc ttacttatgc caggaaaaag taaagagaat
    33661 attcatctta aattattatc atctctttca agaaaattaa taaataaaga atttaaagat
    33721 tctcttttaa aaagtgataa tgttgaagaa atatctaact taataaatga agctttaggt
    33781 ttataattaa acactaaggt tataaaataa aaaaataatt aactaaaaat aggaggaatt
    33841 tgaaatgaat aaaaaattag tagcattatg tgcttgtcca atgggactag ctcacacttt
    33901 catggcagca gaagcaatag agcaagcagc aaaggcatta ggttatgaag caaaagttga
    33961 aactcaaggt gctgatggtg tacaaaatga gttaacaaga gatgatatat taggagcaac
    34021 tatgataata catgcagtag caataacacc agaaggtatg gaaagatttg atggttgtga
    34081 agtttatgaa gttgaattac aagaagctat aaaaaatgca gaaggtgtta taaaagaaat
    34141 agaagaagat ttaggaatat aataaattta ttgtggagga aatagaatgg caattaagaa
    34201 aaaagttata aacagttcag gtgcttctac aacaggagga aataatgtag gaaaatctac
    34261 tccatctagt ccaactcaat caaagggaaa tggaaaatgg aaagaaataa gtaaacatat
    34321 aatgacaggt atatcatata tgataccagt tttagtaatg ggtggactta taggggcctt
    34381 atctcaatta ataccatatg ctatattagg acttgaccca tctgtaggta tagttgatgc
    34441 tatgaactca ggagaattta cagggttcaa gcttagtctt ttaaatatag cacaattaat
    34501 gtcaaatttc ggatttactt tgtttggatt tgcgatacca ctgtttgcag cattttgtgc
    34561 aaactctata ggtggaaaaa cagctttaat tgcaggattt ataggtggat atatagctaa
    34621 taaacctgta ggtgtaccac aattcgttga tggacaatgg actgaagttg ttccagttgc
    34681 atcaggattt ttaggtgcaa tactaatagc attcataata ggatattttg taaaatggtt
    34741 aaataaatct ataaaagttt ctcataactg gttagctttt aaaacaacat ttttaatacc
    34801 attaatagcg tcattagctt gtatggtatt gatgatattt ataataactc catttggtgg
    34861 attaattaat gaaagtatga aaaacttctt aacagcagca ggagcagctg gagaatatgt
    34921 atatgcaaca gctttggcag cagctacagc attcgactta ggaggaccaa taaataaagc
    34981 agcaggattc gttgcacttg gtttaactac agagaatgta cttccaataa cagcaagaac
    35041 aatagctata gttgttcctc caattggact aggattaaca acattattag ataaaagatt
    35101 agtaggaaga agagtatatg atagacaatt ctatcaagca ggaaaaactt ctatattctt
    35161 atcatttatg ggtatatctg aaggagcaat accatttgca cttgagagac caggttttgt
    35221 tataccttta aacatagttg gaagtgtaat aggagctatt acaggtataa ttctaggagc
    35281 aattcaatgg ttcccagagt cagctatatg ggcatggcct ctagtagata acttatttgg
    35341 atatataata ggtatagcag ttggtgctat attcatagca gtaggaaata tattctatag
    35401 aaataaatta ataaaagatg gtaaacttgt tgtagattat atcgactagt atttaatttt
    35461 tactatctat aaataacttt ggctggtaat atggttttaa tcaatattac cagccttacc
    35521 tatatgatta attaaaggcg gtggagataa gatatggata agatagaaaa agtaagagag
    35581 tattttagag aatatgatat agatggattt cttataaata gtcctacaaa caagttttat
    35641 gttggaaatt tatttagctc ttcaggttat gtttttataa cgaaagaatc acaatatata
    35701 atagtagatt ttagatattt tgaagaaatt aaaagaaaat ctagtttgtt taatgtagta
    35761 ttaatggaca aaactagaac tcattttgat ataattaatg atatttgtag agagcagaat
    35821 ataaaggaaa ttggatttga gggaaatgaa gtatcttttg atttatatag aagtatgagt
    35881 aataagttaa gtgcaacctt aaaatcagtt gatttatcta cattaaggga aacaaaaaat
    35941 gaagacgaaa taaaatatat aaaaaaagct tgtgaaatag ttgatgctac attttatcat
    36001 atagtagact ttataaaggt tggtatgact gaaaagcaag ttgaaaatga aattgttaga
    36061 attataaaag atttaggtgg tcaaaaagaa tcttttgata caatagttgc atctgggctt
    36121 agaggagcat tacctcatgg aaaagcaagt gaaaaagtta tagagtatgg agattttgtt
    36181 acttttgact ttggtgctaa gtataataat tattgttcag atattaccag aaccatttgt
    36241 atgggtacta taaataaaga gttagaagaa atatataata tagtaagaaa agctaatgaa
    36301 gagtgtataa gggttttaag gccgggaatg actactggag agatagataa agttgcaaga
    36361 aacataattg gttcatatgg atatgccaat aattttggtc ataatctggg tcatggtgta
    36421 ggaattatgg ttcatgaata tcctgcatta gctcctgaat caaatgaagt attaaaagaa
    36481 ggaatgattg taactataga gcctggtata tatgttccaa gtcttggagg cgtaagaata
    36541 gaagatgatg tacttataac acaagatgga tgtatgagat tgactacatc tactaaggat
    36601 ttaatagttg taagctaaaa ataaattaga taaatatttt ataacaataa gaaaagaaga
    36661 ggtaaattta aatgtataat tttgatgata aaccagatag agtaagtgaa aagtgtagaa
    36721 aatgggattt aaatattata agagataaat ttggagatat tagagaagat tttataccaa
    36781 tgtggattgc tgatatggat tttaaaatac caacagaaat agaaaacaaa tttattgaag
    36841 ctgtaagaag aggtgtattt ggatatacat actgttatga tgaattttat gattcagtta
    36901 ttggttggca aaaaaatatg catgaggtaa atgtagaaaa agaatggatt acattaactt
    36961 atggaactgt atcaacactt cattatgtag tacaagcttt ttgtaagaaa ggtgatagta
    37021 taatattaaa tacaccagtt tatgacccct ttgaatcatc agcaaaaaaa caaggtgtaa
    37081 atgtaatttg taatacatta gatgttgtaa atgatagata ttatataaat tttgataaat
    37141 tagaagttca aattaaagaa aataagccta aattaatgat gttttgtaca ccacataatc
    37201 catctggaag gatatggaca attgaagaaa tgacaagagt agcaactata tgtaaagaaa
    37261 ataatgttat tttggttgca gatgaagtac atgcagagca tattcattat ggtaaattta
    37321 attcaatact taagattgga aaagagcttt tagaaaatgt tattctactg acatcaccaa
    37381 ataaaggatt taatcttggt ggattaaaga cttcttattc aatagttata aataaggata
    37441 taagggacaa atttagaaat aaattaaagc aaaattctat aacttctcca aatgtgtttg
    37501 gaataatagg attgataacc gcttataatg aatgtcatga gtggttaagt ggtgtaaatg
    37561 aatatataaa atcaaattat gaacttttag aaacttgggt aaataaatat aataagataa
    37621 aattgatgaa aatggaatca tcttacttag cttggatgga tataagtgga ttaggtatta
    37681 gtgcaagtga gtttacagat aaattggcta tagatacagg agtattatta gaagatggga
    37741 gtcattttgt aaaagatggt gagaagtatg ttagaataaa tttaggaact caaaaggaaa
    37801 atgtaattga agctttaaat agaatggatt tatttttaaa gtctttataa aaatttggtt
    37861 gaattagtat tttatataaa tttaaactat taatatggta tatacataag tagaatttgt
    37921 tttagattac ttaagtatat accatatttt atataaaaat taaatttgca ttagtcatgt
    37981 gataactatt taaattcata tgtattttgt atgtataata gatataatta ttatttaaac
    38041 ttattgttta tttaattttt caatagaaaa agatttatct gtaagtttaa aattatcttt
    38101 tataccctta gttaagtata ctttcttatc cttagttatg aatatagcat ctacattatc
    38161 aattttatta attaaatcca tacctttttc aagaccaagt ccaaatgtag aagtggataa
    38221 ggcatcacaa ttaatagatt tatcagagat tattgtaaca ctacttaaat tgttttcaaa
    38281 aggataccct gtaaatggat ttaacatatg atgatatatt tttccgtttt tttcgataaa
    38341 tctttcataa attccagaag taacaacaga cttatttgta gtttctatgt ttcctatgga
    38401 ttcaccattt ttactagttg ggtcttggat acctatttta aaaggtttat catttttacc
    38461 attttctaaa gtaaatatat ttcctcctaa gtttactaaa ccttttttta cgtcctctga
    38521 ttttaaatag gctacaattt tatcagcagc atatccttta gctatggcac ctaaatcaat
    38581 tttcatattt tctttggtta attttataga tttatttttt tcatccaata tgatgttctt
    38641 ataatcaata agaggcagaa gtttttgtat ctcagaatca cttggaactt tagctttatc
    38701 tgttccaata gcccataaat ttgataatgg accaattgtt atgtcaaaat atccatctga
    38761 taatttggaa tattgtatag cttcttttac gacgaaaaat gtatcatcag aaactctgac
    38821 aaatttttta ccagcattat catttatttt agaaacatca cttcctggta tatgagtgct
    38881 cattttgttg tcaatatatc ttaaaattga atcacattca tttaagattt tatcactttt
    38941 ggattctttt acattaaata cagtaacttc gtttactgta ccaaggtagt agctagtttt
    39001 actatattta gtattctggc ttgaactcct aaaaaatgaa aataaaatta atgctaaaat
    39061 caatatcaca aaaataaatg tgattttttt cttattcatg gtatattcct cctatgaaat
    39121 tgtgttatta ttttattata acataatttc ataggaggac aaaaaatgaa gaaaaaagac
    39181 attcttctta ttttaggcat tttggttgtt attacagcat gttatgggat aataaatgtt
    39241 ataaattcta aaaatgctgg aaatattgag atttatgtag ataataaatt gtataaaact
    39301 gtttcaataa atgccaaaga agaatttaaa atagagaata gaggggggta taacatagta
    39361 aagattcatg ataaaggggt tgagatagta gatgcttcat gccctgataa ggtttgcgta
    39421 catactggat ttataaacaa gccaagtcaa agcatagttt gtatacctaa tagagtgagc
    39481 ataaaaataa atacaaatga aaagaatgat aatcaagaag atataatttc taaataaaat
    39541 aataaaaata attgaaagga attggtgata attatggtta acttaggaaa cgattgggat
    39601 gaattattga aagaagaatt tgaaaaagat tactacttaa acttaagaaa atttcttata
    39661 gaagaatata aaacacgcca aattttccca aatatgcaca atatatatga agctttaaaa
    39721 cacacttcat acaaagacac aaaagtctta atattaggac aagaccctta tcatggagat
    39781 aatcaagctc atggtttagc tttttcagtt caaccacaag tgaaaactcc accatcttta
    39841 ttaaatatgt ataaagaatt aaaagatgat ttaggttgtt ttatacctaa taatggatat
    39901 ttaatgcctt gggcagacca aggagtgtta cttttaaata ctgctcttac tgttagagcg
    39961 catgaagcta attctcataa gaacaaagga tgggaaattt tcactgatag agtaatatct
    40021 attttaagtg agcgtgaaga cccagttatt tttgtactat ggggaagtaa tgcacgtaaa
    40081 aaagtagagt tgattgatac aagtaaacat tatattttgg aagcacctca tccaagccca
    40141 ttgtcagcaa gtaaaggctt ttttggatgt aaacacttct ctaagataaa tgaaattttg
    40201 aaaaagctag gaaaagaacc tattaattgg cagatagaaa atatataaaa gtaaaaggta
    40261 acaaaatgat aacaaaacaa caataaacaa tgacattgtt gtaactaata gaaatatgct
    40321 taaatagttg aaaatgctat gtatgcatat ttctattttt ttgtcaagtt gacaactctt
    40381 gaaaaaatag ttacaattta gttacacaaa aaacacaaat aaaaaaagaa acttacaaag
    40441 cagaatttta tgaagcaaaa tatatagtgt aaaaaatctt agggaggaaa ttttatatga
    40501 attttaaaaa attgagtaat gctaaagtct cggtaatggc agttgtttta tcagttggaa
    40561 tattatcggg atactctgcg cttaataaac aagtaacatt agtagtaagg ggagaagaaa
    40621 gaactatatc aacatttagt tctaatgtag gagacctttt agaatctcaa aacattagct
    40681 atgatgcaaa tgatatagta actttagatg aaaatacaaa gttatctaat ggagataaaa
    40741 tagaagtaat tgatgtaaag gaaaaaacag ttgtagaaac taaagacatt ccatttgaag
    40801 ttacagttgt tgaagatggc tctttattaa agggtgattc taaggttaaa gaagaaggaa
    40861 agtctggtaa aagtgaatta atatataaag aaacttatca caatggaaag aaagtagaaa
    40921 aggaatttgt gaaaaaggtt gttaaagctg agccagtaaa taaggttgta agcaaaggga
    40981 caaaggtaga aatgcaagta gcctcatcaa ggggagaaag ttcaagaaga atggcttcat
    41041 catcatctag tagcagtagt agtaacaatg gtaaaaatat gagagtggta gcaactgcat
    41101 atactggtca cagtataact gcaacaggaa caaaacctaa gtggggaaca atagctgtag
    41161 accctaaggt tataccatat ggaactaaag tttatatacc tcaatttgac atggtgttta
    41221 ctgcagaaga ctgtggtgga gcaataaaag gaaataaaat tgatatctac atgaatgatt
    41281 caaaaacagt ttataactgg ggaagaaaaa ctatagatat acgtatatta aaatagttaa
    41341 ataatttata tttttactgg aaattatatc ttgattatat agagaagaaa ctctttatag
    41401 attctaaaaa tataatttcc agttttttat tttggatatg taataaaaca ggtaaatttt
    41461 aaccatgtat agataaaagc tatttaaatt ataatttact atcatataaa tctataaaaa
    41521 ttgttgcaaa gagtcaaaat atatgatatt atagatttcg ttgagaagga tttcattatg
    41581 ggagtggatg ggtgccggtg tgccctctag tcttcaaaac tagtatgagg ggttaagagc
    41641 ctcttaggtg ggttcgattc ccacgcattc ccgccaatat gtaaaaggta tcttttaatt
    41701 aaaagatacc tttttaaatt tattttaaga taatttattt ttaataatat ataatgtttt
    41761 gtattatata atgtaaattt tctaatacta ttatatattt agataaaaat ctaggagtgt
    41821 ggaaagatgg aaaataattt tacaacgaga actagttttc tagttggaga tgatgggata
    41881 aaaaaactta ataattcaaa tataatagta tttggagttg gaggagttgg tagttttact
    41941 gttgaagctt tagcaagagc tggagtaggc aatattacta ttgtagattt tgatgatgta
    42001 gatataacca atataaatag acaaatacca gccctacatt caactgtagg aagatacaaa
    42061 gttgatgtca tggaagaaag aatattagat ataaatccta atatcaatat aaaaaaaata
    42121 agaagtcttt ataataaaga tactagtgac gaaatactaa ctgaaagata tgattatgtt
    42181 gtggatgcga tagacatggt aagttccaaa atacatctaa ttgaaacttg tgaaaaaaaa
    42241 ggattgaaaa taataagttc tatgggtatg ggaaataaat tagacccaac taaaatagtc
    42301 gttacagata tacacaaaac aagtacttgt cctttagcta aagttatgag gaaagaactt
    42361 agggatagag gtatcaaaaa gttaaaagta gtatattcta ctgaacaacc aatagaactt
    42421 aagaagaaag taatgaatgg tagaaaagta acacctggta gtgtatcatt tgtcccatct
    42481 gtaggaggtt taataatcgc atctgtaata gtaaatgaac tattaggaca ataagtttta
    42541 aatattttaa tttataaatt ttatatacaa atttgaaggc tagaagcatg aatataaagc
    42601 aattttatat tcatactttt agcctaatta ttttatatta ccttatttta tgtagttgta
    42661 gttttatttt ttagattatg tatctatttc ataattatat tttgtaaata taatttctcc
    42721 atcactataa aaaacaatat ctcctattct atctgttcta taaattgata catggttttg
    42781 ttttagatta ttaataacct ccctatgtgg atgaccatat tgatttttat atccacatga
    42841 tattgcagct atatctggac taattttttt tataaattct aaagatgaag aagaattact
    42901 tccatgatga cctattttta taaaattaat attatttaat ttaaaagaat gtaaaatttc
    42961 tttttcattt tcttcttcag catcacccat aaataaaaaa gaattatcat taaaagttaa
    43021 tttgaaaact atagaattta aattagattc ttcttgtata tatgatggac ttaatacata
    43081 tatatttata ttgttatcta tgtttaaaac atcattttta tagagatgct gaatagataa
    43141 gtttttatcc gtacaagaat ttattagatt ttgataagcc tcactatcag ttgattgttc
    43201 aggcatatat atggagttta cattaaattt ttttataata ttatcaagac ttcctatgtg
    43261 gtctgaatct ggatgagttg ctattataat atcaatagtt tttattcttt tttgtctaag
    43321 ataactaata attatatttt cgctatcttc atcacctcca tcaattaata tattcttatt
    43381 tgtgggtgtt tgaactaaaa tactatcacc ttgtccaaca tcaatcatat gtatagatag
    43441 tagagatttt ttgtcacagc cgattaaaag tgatattata ataagtattg ataaaaaaat
    43501 tttatttctc aataaaaaat cactccattc attacatgtt tatataaatt tatgatgggt
    43561 aatataagat atatatgact tattatattt tatattttaa acaatataaa tgaaattaat
    43621 accataataa aattggagtt ttttatatat acaaaataaa atttttaaaa ttaagcaata
    43681 aaaaaggtag gaggatttta tgtttttcaa agatattgaa ctaaattcaa aaaaagagtt
    43741 agacccatat tttgatttag tagattatga agcttgtgag tactgtttta gtactttata
    43801 tatgtggcaa catgtttata agacaggata ttatataggt gaagattttg ccgttttagt
    43861 tggggaatac gaaggagata gcttctcaat attaccactt gctaaaaaag ataaacttcc
    43921 agaagttgta gattttgtcc ttgaatattt ttctaaaaat aataaaaaaa tatacttaag
    43981 aggtattact actgaagtag tagagttttt aaaagaaaaa tatccaggta gatttgagta
    44041 tatagaagaa agagatttat ttgactatat ttatgatgca gaaagtttaa gaactctagc
    44101 tggtaaaaag aatcaaaaga agagaaacca tataaactat ttcttaaaag agtatgcagg
    44161 tagatatgaa gctaaattat tagataaaga aaactttgat gagtgtttag tactaatgaa
    44221 ggaatgggaa agtaataagg aagaaaataa tgaatttgat gaaagtatgg atgatgaatt
    44281 aataggtatc aaaaagatat ttaaccatta tgacatatta aaagataagg taaaagtgtt
    44341 tggtgtttat gtagatggaa aattagaagc ttttagtata ggagaacttt taaatccaaa
    44401 tatggcattg attcatatag aaaaagctaa tcccgatata agaggtctgt atccatttat
    44461 aaatcaacaa tttttggtaa gtgaatttaa ggacgttgag tttgtaaata gagaagaaga
    44521 tttaggtatt gaaggattaa gaaaagctaa actttcttat catccttgca gatttgttga
    44581 aaagtacagt gttagggagg cttaatagac tatatggaga ttaggtatgc taaagaagaa
    44641 gagatagaaa gcataaaaga gatttggagt tattgtttta acgatacgga gagttttatg
    44701 aagtactatt ttaatgataa atataaatct gaaaatactg tagtagcttt agatgaagga
    44761 aaaattattt cttctcttca gctaaatcag tataagttat tactaaatag caaggtatat
    44821 aatacatcat atgtagtagg agtttctaca cttccagagg gaagaggagc tggatatatg
    44881 aacaaagtaa tgaaatttac tttaaacgaa ttgtataaaa aagggcaatt agtttctata
    44941 ttgatgccta ttgactatag actttataga agatttggat atgagcattg ttacgaccag
    45001 attgaatata ctattaacac agatgattta aaaaatttta agtcaagtgg aaaaatgata
    45061 aagtctaatt tatcacaaat tgatgatttg atacaaatag atagaacttt tttaaatgag
    45121 gttaatggta atgtgttaaa agatgaacat tattatgaaa acttatttaa agagattcaa
    45181 agtgaagatg gatttttata cattcatgaa ggaaatgaaa aagatggata tatagtttat
    45241 tttcttcagg aagataaaat gtttgtcaga gagttatttt ataaaaatat agatgccctt
    45301 aaatcaatgc ttaaatttat atataatcac aatactcaat gtaagatagt tactatatct
    45361 actcctacaa tagataagat aaggtttata ttagacaacc ctaaagatag tgatataaaa
    45421 ataaaacctt ttatgatggg aagagttata aatgtaaaga agtttattga agatatagat
    45481 attgagaatg atattaatag ctcatttaat ttattaatag aagacaagtt tatagatgaa
    45541 aataatggtc tatttaaaat ttctatacag aataagaagg tatctgtaga gcagttagat
    45601 aaaaaaggtg cagaaaagcc tcaagaagat tttgatatta aattagatat aaatacctta
    45661 acacagttat cattttctta tatagatgta aatgaagcta ttttcttaaa tgatataaaa
    45721 gatgtttcag aagaaacact agaaacactt aattgtattt tttctaaaaa a
//
LOCUS       DAGSWI010000015        45569 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.015, whole genome shotgun sequence.
ACCESSION   DAGSWI010000015 DAGSWI010000000
VERSION     DAGSWI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.015"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            118..777
                     /gene="cprR"
                     /locus_tag="MJF80_000771"
     CDS             118..777
                     /gene="cprR"
                     /locus_tag="MJF80_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBZ0279445.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            988..2046
                     /locus_tag="MJF80_000772"
     CDS             988..2046
                     /locus_tag="MJF80_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBZ0279446.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            2297..4441
                     /gene="fdhF"
                     /locus_tag="MJF80_000773"
     CDS             2297..4441
                     /gene="fdhF"
                     /locus_tag="MJF80_000773"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2711..2713,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBZ0279447.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            4490..5323
                     /gene="fdhD"
                     /locus_tag="MJF80_000774"
     CDS             4490..5323
                     /gene="fdhD"
                     /locus_tag="MJF80_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBZ0279448.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            5334..5507
                     /locus_tag="MJF80_000775"
     CDS             5334..5507
                     /locus_tag="MJF80_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279449.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDENN"
     gene            5544..6095
                     /locus_tag="MJF80_000776"
     CDS             5544..6095
                     /locus_tag="MJF80_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBZ0279450.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            6120..7496
                     /locus_tag="MJF80_000777"
     CDS             6120..7496
                     /locus_tag="MJF80_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBZ0279451.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            7496..8011
                     /locus_tag="MJF80_000778"
     CDS             7496..8011
                     /locus_tag="MJF80_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBZ0279452.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(8089..9372)
                     /locus_tag="MJF80_000779"
     CDS             complement(8089..9372)
                     /locus_tag="MJF80_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HBZ0279453.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            9560..9982
                     /locus_tag="MJF80_000780"
     CDS             9560..9982
                     /locus_tag="MJF80_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBZ0279454.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            10084..11022
                     /locus_tag="MJF80_000781"
     CDS             10084..11022
                     /locus_tag="MJF80_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBZ0279455.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFAAEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            11208..13232
                     /gene="ligA"
                     /locus_tag="MJF80_000782"
     CDS             11208..13232
                     /gene="ligA"
                     /locus_tag="MJF80_000782"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBZ0279456.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKKIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            13392..14741
                     /gene="rph"
                     /locus_tag="MJF80_000783"
     CDS             13392..14741
                     /gene="rph"
                     /locus_tag="MJF80_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBZ0279457.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            14741..15211
                     /locus_tag="MJF80_000784"
     CDS             14741..15211
                     /locus_tag="MJF80_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBZ0279458.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            15405..16691
                     /locus_tag="MJF80_000785"
     CDS             15405..16691
                     /locus_tag="MJF80_000785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBZ0279459.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            16841..17425
                     /gene="clpP"
                     /locus_tag="MJF80_000786"
     CDS             16841..17425
                     /gene="clpP"
                     /locus_tag="MJF80_000786"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBZ0279460.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            17444..18694
                     /gene="clpX"
                     /locus_tag="MJF80_000787"
     CDS             17444..18694
                     /gene="clpX"
                     /locus_tag="MJF80_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBZ0279461.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(18765..19292)
                     /locus_tag="MJF80_000788"
     CDS             complement(18765..19292)
                     /locus_tag="MJF80_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBZ0279462.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(19280..19792)
                     /locus_tag="MJF80_000789"
     CDS             complement(19280..19792)
                     /locus_tag="MJF80_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBZ0279463.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            19972..22335
                     /gene="lon"
                     /locus_tag="MJF80_000790"
     CDS             19972..22335
                     /gene="lon"
                     /locus_tag="MJF80_000790"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBZ0279464.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            22325..22936
                     /locus_tag="MJF80_000791"
     CDS             22325..22936
                     /locus_tag="MJF80_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBZ0279465.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(23201..24571)
                     /locus_tag="MJF80_000792"
     CDS             complement(23201..24571)
                     /locus_tag="MJF80_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0279466.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(24746..24861)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25149..26228
                     /locus_tag="MJF80_000793"
     CDS             25149..26228
                     /locus_tag="MJF80_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0279467.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            26619..27299
                     /locus_tag="MJF80_000794"
     CDS             26619..27299
                     /locus_tag="MJF80_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBZ0279468.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            27292..28671
                     /locus_tag="MJF80_000795"
     CDS             27292..28671
                     /locus_tag="MJF80_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBZ0279469.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            28842..29927
                     /locus_tag="MJF80_000796"
     CDS             28842..29927
                     /locus_tag="MJF80_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBZ0279470.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            29981..30340
                     /locus_tag="MJF80_000797"
     CDS             29981..30340
                     /locus_tag="MJF80_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBZ0279471.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            30393..31265
                     /locus_tag="MJF80_000798"
     CDS             30393..31265
                     /locus_tag="MJF80_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBZ0279472.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKGHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKDDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            31266..31709
                     /locus_tag="MJF80_000799"
     CDS             31266..31709
                     /locus_tag="MJF80_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279473.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSRHTLPTTDKTREKFSKIKLNEIKKIYETIGINSITKISIDK
                     NKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            31724..32308
                     /locus_tag="MJF80_000800"
     CDS             31724..32308
                     /locus_tag="MJF80_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279474.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            32561..33598
                     /locus_tag="MJF80_000801"
     CDS             32561..33598
                     /locus_tag="MJF80_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBZ0279475.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            33679..34044
                     /locus_tag="MJF80_000802"
     CDS             33679..34044
                     /locus_tag="MJF80_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279476.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            34049..34366
                     /locus_tag="MJF80_000803"
     CDS             34049..34366
                     /locus_tag="MJF80_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279477.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            34414..36018
                     /locus_tag="MJF80_000804"
     CDS             34414..36018
                     /locus_tag="MJF80_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279478.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            36142..37770
                     /locus_tag="MJF80_000805"
     CDS             36142..37770
                     /locus_tag="MJF80_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBZ0279479.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            38035..39384
                     /locus_tag="MJF80_000806"
     CDS             38035..39384
                     /locus_tag="MJF80_000806"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBZ0279480.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            39540..40619
                     /locus_tag="MJF80_000807"
     CDS             39540..40619
                     /locus_tag="MJF80_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBZ0279481.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
     gene            40964..41872
                     /locus_tag="MJF80_000808"
     CDS             40964..41872
                     /locus_tag="MJF80_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBZ0279482.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
     gene            41877..44246
                     /locus_tag="MJF80_000809"
     CDS             41877..44246
                     /locus_tag="MJF80_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBZ0279483.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            44571..45374
                     /gene="proC"
                     /locus_tag="MJF80_000810"
     CDS             44571..45374
                     /gene="proC"
                     /locus_tag="MJF80_000810"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBZ0279484.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEKFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
BASE COUNT        18706 a         4820 c         8252 g        13791 t
ORIGIN      
        1 atagtaatta attattgtaa gctataatat gagagtatta tggttaatgt gaattttaaa
       61 gatattatta aatatataaa ttagaatgct attagatgga taaatggagg agttattatg
      121 tctaagatat tagtagtaga tgatgagtta gatatgttaa agttgattga aaatgtgttg
      181 aaaagagatg gacatgaagt aaaggtaatt agtgatgtag aaaaggctat gaacatggat
      241 tttaattatt ttaatctaat aatattagat gttatgatgc ctaagatgga tggttttgaa
      301 gtatgtaaaa ctataagaaa taaggttgat tgtccaatac tgtttttgac agcaaaaaat
      361 atggaaagtg atattatgta cggtcttggt ataggtgcag atgactatat aaccaaacct
      421 tttggtgtag gagaattaag ggctaggata actgctcatt taaggcgaga aagtagagaa
      481 aaaaagaatt tattaactat ctcaaatgtt caatttaact ttttaggcaa agaagtttca
      541 attgatggta aaaaaattaa ttttactaaa agtgaatatc taatatgtga atttctagca
      601 aaaaataagg gacaagtatt ttcaaaagaa agaatatatg agtcggtata tggttttgat
      661 ggagaaagtg atataagtac tatcacagag catataaaaa atatacgaag caaattaaaa
      721 caatatgata tttatacaat agaaactgta tgggggatag ggtataaatg ggtgtaataa
      781 gtataagtgt tgtttagtga aaatattata tatatctaca aaatgtttgt aaaatattaa
      841 tctatatatt aaaatttaat ttatagatta atattttttg taaatgacaa aattgtaagg
      901 tattttgtta taaaatatta agattcatag tgtatactaa taatatagta tttagtataa
      961 atcttgagca aaggatggtt atttctaatg gatagactaa agaatattag aaagagttta
     1021 ctacagtata catttttatt tttgttaata ggtataacaa tttacctagt gtttaaaaca
     1081 ttggatatta aaatgctatc aaatgttatg gttatggtag ataagaagtt tttattcata
     1141 ggtgctttgg caataatgtg tcacataatg cttgaaggag tagtaatgaa gataattata
     1201 gaaagcactc acaaagttaa tattagattt attggtttta agctagccat tatgggattt
     1261 tattataatt taataactcc ttttgcttca ggaagccaac cagtacagat atatgtactt
     1321 aaaaaatgta aaatgccact tagtaaggca agtgcagttg ttaccaataa gtctattatc
     1381 ttccaaatag tagttacttt ttattgtaca atacttgttt taatgaattt tgctatgtta
     1441 gaaaaacaaa tgaaaatgat tatgcctcta gtgtttctag ggatagccat taattcattt
     1501 gtcttaatta tgataatatt agttatttta aatcctaaaa aaataaaata ttttatgaga
     1561 attactataa agcatttatc aaaaattaag tttttaaaat ttttagataa taagattgaa
     1621 tccatagaaa gttttataga tgattatagt aaagcaataa atttttttgc aaagaataaa
     1681 aaggtattaa tttcaactat aatagtaact tttattcaat taagtgctta ctttagtgtt
     1741 tcattttgga tatataaagc atttaattta caaggttata gctatattta tatactgaca
     1801 ttacaagcat ttttatatat ggcaatatct ccaataccaa cacctgggaa tgtaggagct
     1861 aatgaattag cctttttcac tatattcaaa tcagtattcc ctcaaccttt gatgggatat
     1921 gcagtatttt tatatggtgg atttatgtac tatttgattc tgattggaag tggaatattt
     1981 actgtaatta ctcactatag aatgaaaaat agaattggca gaaatattgt tttaagtgaa
     2041 aactaaataa gtttaaagaa tatatattga gtatattaaa aaatatgtca taagaactag
     2101 aatattttaa ttaaatggtt gaaatataaa gattattatg acatattttt tttgaaattt
     2161 aagaggaatt gtgagaaaat tgttgaattt aatagatagt attgcaaaat atttatgatt
     2221 ttgcatatta taaaaacact cattatcttt aagataaatt ttacttatca ggggataaat
     2281 ctaagggggg aattgtatgg agaaaaaaat tttaacagtt tgtccatact gtggagctgg
     2341 atgccaatta tatctagtag taaaagataa taagatatta agagctgaac cggctaatgg
     2401 tagaacaaat caagaaagtt tgtgtttaaa aggtcgttat ggatgggatt ttctaaatga
     2461 ccctaaaatt ctaacaccta ggctaaaaaa gccaatgata agaaaacaag gtgtactaga
     2521 agaggttgaa tgggatgagg ctataaacta tacagcttca agactaaatg aaattaaaga
     2581 aaaatatgga cctgattcta ttatgggaac aggttctgca agaggacctg gtaatgaagc
     2641 aaactatata atgcaaaaat ttatgagagc tacaatagga actaataatg ttgaccattg
     2701 tgctcgtgtc tgacatgcac catctgtagc cggtctggct tactcattag gtagtggtgc
     2761 aatgtctaat tctataccag agatagaaaa tgcagatgtt ttatttatat ttggatataa
     2821 tggagcagat tcacatccaa tagtggcaaa tagaatagta aaagctaaaa aaaatggtgc
     2881 taaattaatt gttactgacc ctagagtaac tgaatctgca agaattgctg atatacatct
     2941 acctataaaa ggtggaacaa acatggtttt agtaaatgct tttggaaatg tattaataga
     3001 agaaggtctt tacaataaag aatttgttca aaatcataca caaggatttg atgagtataa
     3061 agaaattgtt aaaccatata cagctaaata tgctgaaaag attacaggaa tcccagaaga
     3121 gcttataaga aaagctatga gagaatatgc aaaaggtaaa aaagctatga tactttatgg
     3181 aatgggtgtc tgtcaatttg gtcaagctgt tgatgtagtt aagggacttg catcaattgc
     3241 tctacttaca ggtaattttg gtagagaaag cgttggaata ggacctgtac gtggacaaaa
     3301 taatgttcaa ggagcttgtg atatgggagc tcttcctaat gtatatcctg gctatcaaaa
     3361 tgtaactgat gataagataa gagaaaagtt tgaaaaagct tggggggtaa aattatctcc
     3421 taataatggt tatagtttaa ctcaagttcc agacttagta ctaaaagaga aaaagcttaa
     3481 agcttattat atatttggag aagacccagt gcaaagtgac ccagatgcat cagaagttag
     3541 agaagcatta gatgaattgg aatttgtaat tgttcaagat atatttatga ataagacagc
     3601 acttcacgct gatgttatac ttccagctac ttcttgggga gaacatgagg gagtatatac
     3661 atgtgcagat agaggattcc aattgatgag aaaagctata gagccacaag gagatgtaaa
     3721 accagactgg caaattataa gcgaaatttc tacagcaatg ggatatccta tgaattataa
     3781 aaatactaag gaaatatggg acgaacttag acaattatgt ccaagtttct taggtgctac
     3841 ttatgaaaag atagaaactc aaggatgcgt tcaatggcct tgtaagagtg aaagtatgga
     3901 agataaagga actatgtatc tgtatgaagg tcaaaagttt tctactccta atggtaaagg
     3961 taatttgttc gctgctgaat ggagaccccc tatggaagtt gaagatgatg aatacccatt
     4021 tagtttatgt actgtaagag aagttggaca ctattcagta agaacaatga ctggtaattg
     4081 tagaacactt agttcattag aagatgaacc aggacgtgtt caaataaatt ctaatgatgc
     4141 tgaaaaatta ggaattgaag atgatgaatt agttagaatt agttcaagaa gaggaagtgt
     4201 aataacaaga gcaacagtta ctgatagagt taaggaaggt gcaacatata tgacttatca
     4261 gtggtgggtt ggagcttgca atgaacttac aatagcaaat ttagacccta tatctaaaac
     4321 accagagtat aaatattgtg ctgttaaact tgaaaaactt gaagaccaag aattagcaga
     4381 aaaatgtgta agagaagagt atcaaagttt gaaagacaaa atgacagcta caaatatcta
     4441 atgatttatt agatttaaga tttataaaag gcagaaaggc taatatttta tgaacaatat
     4501 atactcaaaa actaaattac aagaaaatag taaggataaa gtttttgaaa atagttatag
     4561 tctagtggac tacaaatatt taaattcttt taatgtagaa gtaagcaaaa taaatgaaca
     4621 tgaagtatca atagataatg aagaaataat tgcagaatat ccacttagtt ttattttaaa
     4681 tgataaatat ataaatacat ttttatgtac cccatataat ttagaggaac ttatagtagg
     4741 attcttgaaa acaaaaggat acatagaaaa caaaaaaagc ttattaaaat ttgagataaa
     4801 tgagagtgaa cgttttgcaa aagtaactat tcaagagaaa caaaaaaatc aagatgagca
     4861 aattatattt ttaaattcac tagactatat aacatgtgct cctgtaaaga gcaatataaa
     4921 aataaattat gatacgattt acagtataat ggataaaaat ttaaattcat cagaattatt
     4981 taaaaatact ggtggggtac atagcgtatc tgtatttaat tttgataaaa aaatagtcac
     5041 ttgtgaagat gtagcaaggc ataatgcaat ggataaggct attggatttt gtgttttaaa
     5101 tgacataaca ttaaaagata aaattgtggc tgtaagtgga agaatatcat tggaaatgat
     5161 attaaaagca gctatgactc aaattcctat tgtagtatca aaatcagcac cgactaattt
     5221 atcaatagag ctttctaaaa aattaaatat aacgcttatt ggatttgtaa gaggaagacg
     5281 gatgaatatt tatgcaaatc cacagagagt tatcaaaaag tgagaatgaa tatatgtgta
     5341 ctaaaaaaga agagcaaaga tattcaaagt gtgaaatctg tggagtatta attgatgata
     5401 ctgataaggt atataataac acatacaatt tgtgcaaaaa atgtaaacga aatataagta
     5461 ctgagaaaat agtgaaatat ataaatatat atgatgaaaa taactagagg gttggaaata
     5521 tacaaattga aaggtgggat aatatgaaaa ataaaatagg ctcttttgta atagcagacc
     5581 caaacaaatg tacaggttgt agagcttgtg aagtagcttg ttttacaatg cataatcagc
     5641 ataataatgt gggttataca gttggaacag ttgatatacc tgttatacct agactttact
     5701 tagtaaaagg tgacaatttc tgtatgccaa tacagtgtag acattgtgaa gatgctcctt
     5761 gtttaaactc atgtccacaa aaagctatag ttaaagaaaa caatattatg tctgtaaatg
     5821 aagaaaagtg tataggatgt aaaacttgtc tgctagcatg tccttttgga gctatagact
     5881 tacttccaca atatgaagat ggtaaagaag tagaacaagt aattttagat gaaaataaga
     5941 agatagctta taaatgtgac ttatgtaaag acaatgaaaa aatagcttgt ataagtgcat
     6001 gtcctcagga agcattaaaa cttgttactc caatagatga caaaaaagct aagaatagac
     6061 aagctgcact tagtttattg ttaacaaata aataaaggat acaggggtag gtgacgttta
     6121 tgataataaa tatagataag gatttatgta ctggatgtag agaatgttca aaggtctgtc
     6181 cagtaaatgc tattgaaggt gaagagggaa agccacaaga gataaattta gatagatgtg
     6241 ttatgtgcgg tcaatgtgtt caaacttgta aatcttatgc ctcagttata gatgaaggat
     6301 ttgagttttt acaagagaaa aaacaagaaa gagagatacc agagtctata aatgaaccta
     6361 tatttgcagc atacaatgta tgtgatatag ataaggtgaa gaaagcttta agtgacccaa
     6421 atgtattcac tatggtccaa tgtgcaccag cagttagagt tgctttagga gaagatttcg
     6481 gatattattt aggatactta ggtgctggga aaatggcagc agcacttaga gctttaaatt
     6541 ttgatagagt ctacgatact aattttgcag cagatttaac tataatggaa gaaggtagtg
     6601 aattaattaa aagagtgaca gaaggtggaa ctcttccaat gtttacatct tgttgtccag
     6661 cttgggtaaa attcatggaa aaaaattatc ctaaattaac taatcattta tcttcttgta
     6721 aatcacctca gcagatgggt ggtgcaatat ttaaaactta tggtgcagag ataaataatg
     6781 tagatgcatc aaagatattt agtgtttcta taatgccatg tacatgtaaa aaatatgaat
     6841 gtgatagaga agaaatggat tctagtggat atagagatgt agatgtagta ttaactacta
     6901 gagaactagc ttatttaata aaagacatgg gaattgattt taaaaactta agagaagaaa
     6961 aatttgatag tcctttaggt agttatagtg gagctggaac tatctttggt gttactggtg
     7021 gggttatgga agctgctata agaacaggat atgagcttat aactggagaa agtattcctg
     7081 atgtagaagt aaaacaagta agaggagaaa atggttttag aaagtcaact attaaaatag
     7141 gagacttaga cttaagagtt ggagtagtaa gtggattaaa aaatgtaata ccagtgcttg
     7201 aagacttgga aaaaggaaaa ttagatgtag attttattga agttatgact tgtcctgttg
     7261 ggtgtgtaag tggtggaggt caaccaaaaa ttttactaga agaatataag gaattggctt
     7321 atgaaaatag aacaaaagca acatacgtgc atgatgaaca tttagcaatt agaaaatctc
     7381 atgagaatcc tgatattaaa aaactgtatg atgaatatct ggtagaacca ttaggagaaa
     7441 aatctcatca tctattacac actacatatt gtataggaaa ggaagtggct aagtgatgaa
     7501 ctattttgtt atagcagacc ctgataaatg cataggatgt agaacttgta tgataggatg
     7561 tgtagttgca cattcagatg aagatatttt ttaccaaaat ccagatgaaa taaattttaa
     7621 tccaaaactt tctgtcataa agacaaaaga agtaagtgct ccaatacagt gtagacattg
     7681 tgaagatgct ccttgtgcaa aggcatgtcc aaatggtggt atagttaggg ttggtaacac
     7741 aataaaaata aatgaagaaa attgtatagg atgtaagact tgtatgttag catgtcctat
     7801 aggtgcaata gacatagtta cattaaaaga tgtagatgaa ggaaagcttt gctttagaga
     7861 aagaatgaca gcaaataagt gtgatttttg tatagaatcg ccagaagggc ctgcatgtgt
     7921 aaatgtttgt ccaactaagg catttactat agttaaggat gaggatatag atagtaaagt
     7981 taaaaataag agaaaattag ctatattgta atataaaaaa gatggagttt tatttatgat
     8041 taaaagctcc atctttttta ttataatatt tatatatcta aaaatcaatt attgcattag
     8101 tttatctaat ctttctttat tcaatttttt cattcctata gaagttataa taagtaaagt
     8161 ataggcaatt ataactgata aaattataat agaccaagaa aatctgtatc caaaattatc
     8221 aactaacaat ccaaataatg ttgaacctaa tgcaaatccc actgcaaaaa ttaattgtac
     8281 gattccatat atgctaccaa actccttttg accaaagaaa gagccagtta aataagcagg
     8341 accaatcata taagcaaata cagaaagacc tttcaaaatg gcaaatacaa atgcaaaagt
     8401 agaagattta tctaccaata gtaatgataa agttgctata agaaccaata caaaagatat
     8461 aataagtggc tttgtttgac cgaatttatc aaacaataat cctccaaata aattaccaat
     8521 gagagcaaat aaggcaaatg tggagcctac tgttcctatt agaataggat ttagataatt
     8581 tctaagataa gttggatatt gtacagataa tgatgaaaca tatataccaa caaatataaa
     8641 tccaatagtc ataatccaaa aatatttaat ttttttagct tcttttaatg tataacccca
     8701 tactgaagga gaaattgtat cagatgcttt ctctttttta cctttagatt ttaccttttc
     8761 attaatatct ttaggcatcc tgagtatgaa taatattaca ggtataccaa ctattaatgc
     8821 tataattcca aatattagat aagaacgact gtatccttga gttgctaata agcgaactgc
     8881 aagtggttgt agaaatatac taccaataga accaccagca aaagcaaagc ccaaagcttt
     8941 tcctttgctt tcttcatcaa accaagcact tattaaaaca ggtattccta tagcagatat
     9001 aattgtagcg ccaatttgaa caatacatga tacagtgtaa aattgccata gttcagtaca
     9061 tatagaaaaa gtagcaaaag caatactaga aattatacaa cctaataaat atagtatctt
     9121 aatattgact ttagaaaata atacacctat aaaaggtgat gcaattgctg aggcaatagt
     9181 accaatagtg aatattagag aaaaagaagc taaactaaag ccttctcctt ctacaacata
     9241 acttataaat tgaggctgta tacttgctac aatactaaat ggaacagctt gtatcaacat
     9301 acaaccaagt actataaccc aagcatttga aaagttttta atgctaaatt tattgtttag
     9361 taatgtattc atggtatctc tcctttaggt tatgatttgt agatattaat ctaattattc
     9421 taattttata tattagtttt ggatagccca actatttaaa tataatttgg gtattaatat
     9481 aaaaattaac tataaaaatt gattatttta aatgtatggg gtaaattaaa gtatataaat
     9541 aaatgaaagg atggataaaa tgtcatttga aaaaataaat aataataacc tagataaaat
     9601 acacaacagg aagtgtatta tgcttgtaaa ctttaataaa aaagaattat cattaattaa
     9661 aaacgtaagt aactttgctg gaataagaga ccatatcatc ttaagtgata aaaatggaga
     9721 gaatataata agagacattt tagatggaaa tatatcaaat gaatgtgaaa atgggattaa
     9781 gagtaagtct gtaatattta ataacactcc aagcacacaa gtaaatgcat ttttagaagg
     9841 gctaaaaaaa gtgagaataa atagaccttt tacagctatg gtaacagaga catctattga
     9901 ttggacattg aataatttaa tagaaaattt agttcaagaa cgtaatgcta tgtctttagg
     9961 aaaaacaatt gaacataagt aaatttaaga tatcttatag taattttaac taatgtacat
    10021 tgagtatcat attatatttt agaatataaa attttaaatg tgggtagatg gaggagattt
    10081 gagatgaaag ttttatttac aataaattat ggaaaagaaa aatttgataa aataagagaa
    10141 ttaggctatg atgtcataca ttactctgaa gatagtattg aaaataatga ggatgtaaat
    10201 acagcagatg tactagttac atacaatcca tttgagagat tagatatttc taagatgaaa
    10261 aatttacaat atatacagac aactagtgta ggtatagacc aacttccaaa agaagaggta
    10321 ttgaagagaa atattaagat agctaataat agaggcggat atagtgtacc tattggtgaa
    10381 agtattgtat tgtatatttt agaaatatat aaaaatagtg caaaattttt taaacaacaa
    10441 caaaataaag aatggaagat ggatttttct gtatcagaat tgtatggaca aagagttgga
    10501 tttttaggaa caggtactat atcttcagaa gcagcaaaaa gattaaaagc ttttggtgta
    10561 gaaatatggg gtgtcaatac tgatggaagt agtaaaaaat actttgataa gtgttttgct
    10621 gctgaggata tgaatattgt atttgaagaa tgtgatatag tagttgtagc aatgccttct
    10681 acaaaaagta cagatggaat agtaaacaaa gatatgttta aacttatgaa agatggttct
    10741 gtatttataa atgtaggtag aggaaataca gttaatcaga aagatttaga agaatgtgta
    10801 gagaaattta gaggggtagc attagacgta tttgaaagtg aacctttaaa caaggacaat
    10861 aagttgtggg agtgcgaaaa tgtcatagta actcctcata attcttgggt gtcagataaa
    10921 aacaaagaga gaacatttaa tatggtttat aataatttga aacactatat aatggagaat
    10981 aaacctgtag ataatgttgt agacatatca agaggctatt aaaaataaaa aattttgata
    11041 aaaataaaaa ctcttattag tcgtattaaa gatactaata agagtttttt ttgtaataaa
    11101 tttaaagcca aaaaagtgat atactaaaaa atatataaat aggagataat taattatagg
    11161 gaaatttcaa aataatttaa ttttaaaaat atgaagaggt gcaaaaagtg agcgtaaaag
    11221 atgaaattaa taaacttata gagacaataa actaccataa tgaaaggtat tataatcaag
    11281 atagcccaga aatatcagat tatgaatacg ataagttaat gaaggagtta atttctttag
    11341 aagaagaaca tccagaacta aagaaaattg attctccaac aaatagggta ggaggtaagc
    11401 cattagataa gttcaatcaa attgtacata aaactcctat gttgagttta tcaaatgctt
    11461 tttcagagca agatttgaga gattttgata agagagttca agaatatgtg ggagaaaatg
    11521 ttgaatatgt tgttgaattt aaaatagatg gtctttctgt aggtctaact tataacaatg
    11581 gagagtttga aaaaggagca actagaggag atggagttgt aggagaagat atttctcaaa
    11641 acttaatgac agtaaaaagc atacctctta agataaatga taaaaaagaa ctaatagtta
    11701 gaggagaagt atatatatca aaagataatt ttagaaaagt aaatgaacaa caagaagaac
    11761 aagaacagcc attatttgct aatccaagga atcttgcagc tggttcattg agacaattag
    11821 acccaaaact aacagcaaag agaccattag atatatttgt atttaatatg gaaaatatag
    11881 aagaatacga tttagaaagt cacagtgaat cactagagta cttagaaaaa ctaggatttt
    11941 cagtaagtca gagttataaa gtttgtaaaa gtatagatga agttattgag catattgaat
    12001 actggacaga taacaggggt aatttagact acgaaataga tggtatggta ataaaagtaa
    12061 acaatataaa acaaagagac gaaatgggat atacagccaa aagtcctaga tgggcaattg
    12121 catataaatt cccagcagaa aagaagaaaa ctaaaatagt agatataata gtagaagttg
    12181 ggagaactgg tacaataaca ccttcagcaa ttcttgaacc agtcagactt gcaggtacta
    12241 ctgtaagtag agctacactt cacaatgaag attatataag agaaaaagat ataaaaataa
    12301 atgatacagt attagttcaa aaagcagggg acataatacc tcaagtttta gaagttataa
    12361 aagaagaacg tactggagat gaaatagatt ttaaaatgcc agaaaagtgt ccagtttgtt
    12421 cagagccaac aataagatta gaaggagagg ctgctgtaaa atgtatcaat atgtcttgcc
    12481 cagcccaaat aagaagggga attatacact ttgtgtcaag ggatgcaatg aatatagatg
    12541 gattgggaga atctataatt acactgctat taaatgaaaa aattattcaa gatgtatctg
    12601 atttatatta tataaaaaag gaagatgtag tcgacctaga gagaatgggc gaaaaatctg
    12661 ctgacaattt aataaattca attgaaaaat caaaagaaaa tgatttatgg agacttataa
    12721 atggattagg tattaaattt ataggagtta aagcagctaa gatacttgca tataacttta
    12781 aggatttaga tgaagtaatt tctgcaacta gtgagcaact agaagaatta gaggaatttg
    12841 gaagtataat ggcaaacagc gttgtcgagt tttttaagga agacaaaaat atgaacgtta
    12901 taaacaagtt aaaaaatgta ggagtaaata caaaatcatt ggataataca ggtgaaaaag
    12961 tagaaaaaat atttgaaggg atgaaaattg tattgactgg tacacttcct acattaaaaa
    13021 gaaatgatgc aaaagatatg atagaaaaaa gaggtggaaa agctacatct agtgttagta
    13081 aatcgacaac atttgttttg gcaggtgaag aagctggttc taaacttact aaagcaaatg
    13141 atttaggaat taaagttata gatgaagaga gatttttaga attattaaag ttatcatcga
    13201 aagatgaagt aaaagctgta ttagagagtt aaaaagtcgc ttaatttagt agaaaactac
    13261 tataattaat gatagaatag ttcagtaagc taataagatt acgaaaaatg acaataatta
    13321 atatttgaca aaattaaaaa ttataaaaat aaatttataa aaaataaaaa atatgagaaa
    13381 ggatataaaa aatgaccaga agttatgata gaagtaatga acagataaga cctgtaaaga
    13441 taactagaga ttatacaaag tatgcagaag gttctgttct tatagaaatg ggagagacaa
    13501 aggttatatg cactgcatca gtggaagaaa aagttcctcc ttttttaaga aatagtggta
    13561 ctggatggat aaatgctgaa tattctatgc taccaagagc aacacaacaa agaaagataa
    13621 gagatgcatc taggggaaaa attgatggaa gaacacaaga aatacagagg ttaataggaa
    13681 gagcaataag gtctgttgta gacttgaata aattaggaga aagaactatt tgggtagatt
    13741 gtgatgtgat tcaggcagat ggtggaacta gaactgcatc tataacaggg gcatttgtag
    13801 cagttgcaga agcaatatat aaattatata aagcaaagac aataaaagag atgccaataa
    13861 caaattttgt atctgcaatt agtgttggta ttgtaaatgg aatacacatg ctagatttat
    13921 gttatgaaga agattctaaa gcacaggtag atatgaatat aataatgact gataagtgtg
    13981 agtttgtaga agtacagggg acaggagaag agaaaccttt ttcgagagca gatttaaatt
    14041 tattgttaga gttaggtgaa aaagggaata aagaattaat aaggcttcaa agagaagctt
    14101 taggtgaaat atcagatgag atattgggta tggattatgg taatgaagta gtgattgcta
    14161 ctaataatgc tcataagcta gaagaaatag gtgaaatctt aaaagatttt gaatataaag
    14221 tgtactcttt aaaagatgtt gatttagcag gtatagagat agtagaagat ggaaaaacat
    14281 ttgagcataa tgcgttgata aaagctagaa caatagcaaa aaagactaaa cttatagcaa
    14341 tatcagatga ttctggctta gaagtagatg ctttaggcaa aaaacctggt gtttattcag
    14401 ctagatatgc aggagaacat gcaacagatg aagaaaatag aaaaaaactg ttaaaagcta
    14461 tgcaaaatgt accaatgagc aaaagaaatg caagattcgt ctctgcaata gcagtagttt
    14521 ttcctgatgg aaaagaattt gtagtaagag gaatttgtga aggaatgata ggatttgaag
    14581 aaaaaggtaa aaatggtttt ggatatgata gcttatttat agtaaaagga tatgataaga
    14641 cttttggtga aatacctagt gttataaaaa attcaatcag tcatagagca aacgcactaa
    14701 aacttatgaa acaagagttt ataaaaaggg tagtgaagta atgaaaatag gcatagtaag
    14761 cgacacacat atgatgatta aaaatatgga aaaaacaata ccatatttga aagaatgtga
    14821 cctaataatt catgcaggag ataactttac tgattcaaga tatattcata gcatgactaa
    14881 tgtaggtatt attgcagtta aaggtaattg tgactttgat gctgttgagg aagaagttgt
    14941 ctttgaagtg gcaaataaaa ccatattctt atgccatgga gataagtatg gtgtaaaata
    15001 tggaactaat atgttagaaa aaaaggcaac tgaagttgat gctgatatag ttatatttgg
    15061 tcatacccat accccattta gagaaattaa agatggtgta ctttacataa atcctggaag
    15121 cacttcactt ccaagaggag tatcgtataa aagttttgtc attatggata tagaagaaga
    15181 tgacataaaa attgaagaaa tacgcatata aaggtgtatt tatctaaaaa aatggaaaaa
    15241 aacgctcaaa aaattaaatt ttatcaaata agtacaatga tattaatgga acacttgtaa
    15301 ataaaaaaac ttatgtgata taattaagtg gttgaattat aaatagaatg aattataata
    15361 ccgttatcgg gtattacata aatatcacta ggaggattag aaggatgaaa gcagaactaa
    15421 ttaaaaaaga gggtaataaa gttactttaa aaataacagt agataacaat aaatttgaag
    15481 cagcagtaaa taaggcttat aataaaagca gaaataaata caatatacct ggatttagaa
    15541 aaggtaaagc tccaagaatt gttatagaga ctcaatatgg aaaaggtata ttctacaatg
    15601 atgctataga aatattattc ccagaagttt atccagaagc tatcaaggaa ttagacatag
    15661 acccaataga taatccagat ttagatatag aagaaataag taaagacaat gggttggtta
    15721 tggttcttaa tgtagaagtt aaaccagagt ttgaattagg aaactacaag ggtatagaaa
    15781 tagctaaggt tgagaatact gtaagtgacg aaaatgttga tgctaaacta caagaaatgg
    15841 ttgaaaaaaa tgccagatta gtaagtgtag aagacaaagc tttagaagat ggagatacag
    15901 ctataataga cttcgaaggt tttgaaaatg gtgtagcttt tgatggtggt aaaggtgaaa
    15961 attataactt agttataggt tcaaacacat ttataccagg atttgaagaa caattagtag
    16021 gtaaaaaagc tggtgaagaa gtagaagtta atgttacttt cccagaagaa tatcactcac
    16081 aagatttagc tggaaaacca gttgtattta atgtaaaaat aaatgatgta aaagtaaaag
    16141 aattatctgc acttgatgat gaatttgcta aagacactag tgaatttgat tctttagatg
    16201 aattaaaagc agatgtaaga gctaagttag aagaagaagc taaaaataga gcagatgcag
    16261 aaactagaaa ttcagtagta gaaaaagttg ctgaaaatac agaaatagaa ataccagaag
    16321 ttatgataca acatcaaata gataatatgt taaatgaact taactaccaa ttacaatatc
    16381 aaggatttgg attacaacaa ttactagaga tgactggtag aactatggaa gaattaagag
    16441 aagagagaaa agaagatgct aaaaaattag ttaagtcttc tttagtatta gaagcaataa
    16501 ctaaagctga aggaattgaa gcaacagaag aagaatttaa ggctgagtta gaaaaaatgg
    16561 cttctgctta caatatggaa gtagaaaaaa tagaagcttc tttaagagat gccgacaaag
    16621 aagatataaa aggtcaaata aaaataagaa aaacaataga tttattagtt gataatgcaa
    16681 caatagctta ataaaataaa cgctaactaa tatagttgac gttatgaaat gtttagtata
    16741 taatgatgtg caaatataat ttagttaaat gcgtataata atttacagta gcttaaaaaa
    16801 agctactgta aattatatag aataaagggg gtatgaaacc atggcattag tacctgtagt
    16861 tgttgaacaa acaggaagag gagaaagatc ttatgatatt ttttctagac tattaaaaga
    16921 cagaataatc ttcttaggag atcaagttaa cgatgccaca gcagggctta tagtagcaca
    16981 gctattattt ttagaagctg aagacccaga caaagatata catttatata taaattctcc
    17041 aggaggaagt ataacttctg gaatggctat atatgatact atgcaataca tcaaaccaga
    17101 tgtaagtact atatgtatag gtatggctgc ttctatggga gcattcttgt tggcagcagg
    17161 agcaaaagga aaaagactag cactaccaaa tagtgaaata atgatacatc aaccattagg
    17221 tggagctcaa ggtcaagcaa cagatataga aattcatgca aagagaattt taaaaataaa
    17281 agaaacttta aatgagatat tatctgaaag aactggtcag ccattagaaa aaattaaaat
    17341 ggatactgaa cgtgataact tcatgagtgc actagaagcc aaagaatatg gtttaataga
    17401 tgaagtattt acaaagagac cgtagataga gagaggtgtt aatatgtcta aatatgaaga
    17461 aaaacgacaa ttaaagtgtt cattctgcgg taaaaatcaa gatcaagtaa gaagattaat
    17521 tgcaggtcca aatgtatata tttgtgacga gtgtgttgaa ctttgtgacg aaattattca
    17581 agaagagata gaagatacta tagatgagga tactacatct ttaccaaaac caaaggaaat
    17641 gatggagatt ctaaatgatt atgtaatagg tcaagaaaaa gctaaaaaag ctttatcagt
    17701 tgcggtttat aatcattata agagaatata cagtaaaaaa tctagtagta aggatataga
    17761 aattcaaaag agtaatatac ttttattagg acctacaggt tctggaaaga cattacttgc
    17821 tcaaactcta gcaagaactc taaatgtacc ttttgcaatg gcagatgcta catcattgac
    17881 tgaagctgga tatgttggag aagacgttga aaatatactt ttaaaactta ttcaagctgc
    17941 tgattttgat atagaaaaag ctgaaagagg tataatatat atagatgaga tagataagat
    18001 tgcaagaaaa tctgaaaacc catctataac tagagatgtt agtggagaag gtgttcaaca
    18061 agcacttcta aaaatattag aaggtactgt tgcaaatgtt ccaccacaag gaggaagaaa
    18121 acatcctcat caagaatttt taaagataga tactactaat gtattattta tattaggtgg
    18181 agcttttgat ggattagaga agataattca aaaacgtggt ggagataaaa cactaggttt
    18241 tggagctaaa atagaaagta aaaaagaatt agatttaggg aagctatatg aaaaagtact
    18301 tccagaggat ttattaaaat atggtataat accagagttt ataggaagaa taccagtttt
    18361 agctacttta gagttgttgg atgaagatgc attaatgcag atattacagg aacctaaaaa
    18421 tgctctagta aaacagtata aaaagttatt ggagttagat gatgttgagc tagaatttga
    18481 agaaggtgct cttcgtgcaa tcgctaaaaa ggccatagaa agaaatacag gagctagagg
    18541 acttagaagt atagttgaaa gcgtaatgat ggaaactatg tttgaagtac catctagaga
    18601 taatataaag aaggttatag taacagagaa atctgtgaac gaagactcag taaatcctat
    18661 catagtactt aaggatcaag aggaaagtgc ataagatgat gaaaaggagc tttaaaagct
    18721 ccttttttta tgttaatatg ctaaatataa aataggtatc attattataa aaatgtgagt
    18781 aatataccta gaataccaaa gacgaataca attgcaatgg aaccaatttt tcctttatat
    18841 aaaagataaa atgatgctat aaatgcaatt ataccatata agtcccaatt aacttctttg
    18901 gcagatgaaa aagctgctga taatatgaat cctagagtaa ttggtctaag aactgtcaat
    18961 aagttttcta cctttttatt atttttaaat ttagaaaata actttgatat aatagttaga
    19021 cctattaatg caaaaattgt aacaccaata gttgcagcta gagaaccaaa gaagccagca
    19081 gtcttaaatc ctacaaatgt agcactattt attgctacag gacctggtgt agattgtgat
    19141 atagcaagta aatccaaaaa ctcttgatta gttataaagt gatatttaga tataaattct
    19201 tgctggataa atggtagcat ggcataaccg ccaccaaaac taaatgcccc tattttaagg
    19261 aatattaaga agagtagtat tatttctgac atagactata tacacctcct ccaattatta
    19321 aataaatagg gcttatattg aatacaccaa cagataaaac tgctataatt aatagtagta
    19381 tgttagtttt agactttggt agtttcttga acatgctcgt aaaagaatac aatataagag
    19441 ctattacaac aggcgcaact cctttaaaaa atccatctaa aattttagat gatgaatttt
    19501 taaagtatag atacgataag actaagacta tacaaaagga tggtaaagca gagcctaatg
    19561 tacatataag aactcctggt ataccataaa gtttatatcc taataataca gatatattaa
    19621 ctgccagtac tccaggataa ctttgagcta tggataaata atcaataaat tcttcctgct
    19681 ctaaaagttt ttgtttgttt acaagttcat cttgtataag aggaaccata gcgtatccac
    19741 caccaaatgt aaacgcacca atttttaaaa aggttaaaaa caatttcaac ataatagaaa
    19801 cctccaaaag ataaataaca tctaagtata ctaatattaa tattatatca taataaaaag
    19861 ataatatagt atgatagtat tctaagaatt tatcattaat gttgaaaatt aacttgttca
    19921 aatatataat attaaaagca aagcaattag actagaaaag gagtaatgtt gatggaacaa
    19981 aattatacta aaatagatca tgaattacca ttaattcctc taagaggatt agctatattt
    20041 ccatatatga tattaaattt tgatataggt agagagatat ctcttaaagc tttagaccaa
    20101 gctatgatgg atgaagaact tatattttta acttctcaga aagaagcaga ggtagatgag
    20161 ccaggagaag aggattttta tcatgtggga acaatctgta aagttaagca aatgataaaa
    20221 ttaccaggag atacagttcg tgttttggtt gaaggtgtat caaggggtag agtaaagaaa
    20281 atagaacaag aagatgggta tttcagagca gtaatagaag aaatagtatt tgatagtgat
    20341 aatttggata gtgaaacaga agttgaaatt gaagcatttg taagaaatgt atttgatgcc
    20401 tttgaagagt atataaatat tggaaatagg gtatcaccag aaatattaat atctttagct
    20461 gatatagaag atgtagatag atttatagat acgatagctg caaacatata tctaaaatca
    20521 agccaaaaac aagaaatatt agaagaattt gatattagaa aaagacttga acttatttat
    20581 tctatattat tagaggaaat agatatatta aaaatagaaa agaaaataac tttaagagta
    20641 aaaaaacaga tgaataaggt tcaaaaagaa tattatctta gagaacaatt aaaagctata
    20701 cagaaagaat taggtgaaga agaagatata aactctgaag cagatgagta tagagaaaag
    20761 cttaaaaaaa taaaagcacc taagacaact aaagagaaaa tagaaaaaga gatagataaa
    20821 ttttcaaaaa tatcttctat gtctccagat gtatctgtaa gtagaaacta tttagatact
    20881 atattctcat tgccatggaa taaagagaca aaagataaat tagatataac taaggcaaaa
    20941 gatatattag atgaagacca ttatggatta gaaaaagtta aggagagaat actagagtat
    21001 ctagctataa gaactcttgc taagtcactt aaagggccta taatatgtct agttggtcct
    21061 ccaggaacag gaaagacatc tatagtaaaa tcaatcgcca gagcattaaa tagaaaattt
    21121 gtaagaatat ctttgggtgg agttagagat gaagcagaaa taagaggtca tagaagaact
    21181 tatgtaggtt caattccagg aagaataatt aatggggtaa aagaagccca aacaaaaaat
    21241 cctgtattct tatttgatga gatagataag atggcagcag attataaagg tgacccagct
    21301 tcagcaatgc tagaagtatt agacccagag caaaataagg attttgtgga ccattactta
    21361 gaaatacctt ttgatttgtc aaaaatacta tttgttacaa cagcaaatag tctaagtaat
    21421 ataccaagac cattattaga tagaatggaa gtaatagaag tatctggata tatagaagaa
    21481 gaaaaattaa atatagcaaa aaaataccta cttccaaaac aaattaaaga acatgcttta
    21541 aaagaaaact ttataaaaat agatgatgaa actttaagaa gtataatcaa ccattataca
    21601 agagaagctg gtgtaagaac actagaaaga actattggta agatttgtag aaaggttgcc
    21661 aaaaaatacg ttgaagaccc aactcttgaa gaggttgtta taaataagtc tgacctagag
    21721 acatatcttg gaaaagacat gtttaagtat caacttgcag aagttaaccc tcaaatagga
    21781 ttagtaaatg gtcttgcatg gacagaagtt ggtggagtta cacttgaagt agaagtaaac
    21841 gtacttaaag gtaaaggaga aattgtatta actggaaaat taggagatgt tatgaaggaa
    21901 tctgcgaaga caggtatctc ttatataagg tcaatagtgg acaagtttga tatagaccct
    21961 gaattttata agacgaatga tattcatata cacattccag aaggtgctgt tccaaaagat
    22021 ggaccatcag ctggtataac tatggctctt gcagtaatct cagcacttac taaaagacct
    22081 gtgcctggaa atatagcaat gacaggagag ataactttaa gaggtagagt cttagcagta
    22141 ggaggagtta aagaaaagtt attagcagct catagagctg gtataacaaa agtattaatt
    22201 cctaaagaat gtgaagctga tttagatgaa ataccagaaa atgtaaaaga aaaaatggag
    22261 tttgtgcttg tagaacatat ggatgaagta ttagaacaag ctttattaaa gagtggtgaa
    22321 aataatgaaa attagaagtt cagagataac tatgagtgct gtgaataaat cacagtatcc
    22381 agcagaagga atcccagaga tagcattggc tggtaggtca aatgttggaa aatcatcaat
    22441 aataaataca ttgcttaaca gaagaaactt cgctagaacc agtcaaacac caggaaaaac
    22501 tagaactata aatttttatc taataaataa tgaattttat tttgtagacc ttccaggcta
    22561 tggatatgca aagatagcta agtctgaaaa agaaaagtgg ggaggtataa tggaaagata
    22621 tctagaaagt agacaagagt tatgttctat attcctactt gtagatatta gacatgagcc
    22681 tacagcagat gataagctta tgtatgaatg gataaagcac tttggatata actgtgtagt
    22741 tattgcaact aaggcagata aaatatctag aggacaatat caaaaacaca taagtataat
    22801 aagaaaaaaa ttgcaaatgg aaagtagtga aaaagtaatt cctgtttctt cactaaaaaa
    22861 gactggtgtt gaagaattat gggaagagat tgtaaatcaa tacaatcaac atggatatga
    22921 aataacagta gattaaattt aaagtgttaa atattgacat aagatttatt tgaataataa
    22981 atgtaataat agttagtttt aattatatta atattaaagc aaaatatagg atgccttaaa
    23041 atagttttaa tgaacttttt ttggcatcct tattgtttat aaacaataaa tatcagtaat
    23101 tttgctaaat actaataaat tttagtaagg tatgggaaaa ttagtataaa ttagttcaac
    23161 taaaaaatta ttgacttatg tagtcagcaa taaatagcat ttaagaaata gatttatctg
    23221 tttttctcat aaagaaaaca gttagtataa gccctataaa acatactgat gctgcaatcc
    23281 aataagactg attgctagct ataacatagg ctgatgtgtg acttttaaac ttttgattgt
    23341 atgtaagact ttgtgcaaaa aaactactaa aaaatgctgt tccaagagca aatgataaat
    23401 tacgaatagt tgcaagaaaa ctgcctccgt atccctgttt ttgaggagga acatctgcta
    23461 aaatactact attgtttggt gaactaaatg ctcctagccc aagtccaaaa ataactaaac
    23521 atacaattaa aagatacaat ggagaatctg cttttaaaag tgccattcca actagtgaga
    23581 tggtcataaa tgaaaaagat gctgttagaa tgttttttgc accgtgttta tcagaaagtg
    23641 aacctgcaat aggtgctata agtaccataa taagtggatg tacagtcatt acataaccag
    23701 ccatcatagg agataaattt tttaattgtt ctaaataaaa tggaagtaga aaagatactg
    23761 ccatttgagg aaaataacaa gctactccaa tgatattgcc tacagtaaag tttctatttt
    23821 taaatagagg taggtcaata aagctttgtt caaattttac ttctcttaat gccaacaata
    23881 agaagattat aagtgctgtt atacctaaat acaatcttgt attacctttc tcattcattg
    23941 caagtaatat aagcgtagtg caagatgcga aaagtagaag acctggaata tcaaagctct
    24001 tgttttgttc atccaaaaca ggtttaggca gaaatttaat gccaagaaag actgctataa
    24061 taccaatagg tatattgata ataaaaatag aaggccatcc aaaatgttct aaaattatac
    24121 ctccaattac aggaccactc atatttccta taccaactac agctccagtg attcctatag
    24181 cttttccttt ttcatttgct ggaaatgcat tggaaactat accaagccca gtagccatta
    24241 aaatacttgc tccaactgcc tgaaagattc ttgaagaaat taaagttgat aaattgtttg
    24301 acatactaca cagaaaagaa cctattgtaa atataaaaaa tcctaatgtg tatagacgat
    24361 ttgaacctac tttatcgcca agttttccga aaaataacat agtagctgtt acaacaagca
    24421 tataagctgt tacaacccac tgaatttgtg acatgttaat tttaaagtca gcagcaattg
    24481 taggatttgc gatatttagt atacttccat caagagtaga cataaataca ccaatacaga
    24541 tgataatcaa tactatccat ttttgtttca tgataacact ccaatcttaa tagattttat
    24601 aagtacgata taattgtaaa ataaacaaca aaaaaagcac ctgtatatta ggtaccttga
    24661 atgtgtctaa tttcatgaat aaatacggta gttgaaggat ttgtatagat tactcatgta
    24721 aaattacata aaggtactca tatactattt tcttctacca tccagatttt actgtcggct
    24781 ttggaatatg accaaattat gccataaggc tcgcgggctt ttaccgccga tagggaattt
    24841 cacccgttta tgaagaaaaa cagatgtatt ttgtaatatt attttaacat aaaattataa
    24901 tggaaacaat tggatttaag taatatccag ctgtattaca atacaaatat ttcaatatgg
    24961 acacttaaat tgtatttata gatagaataa ggaacagtat gagaatctat aataattaga
    25021 taaaattaac ctgttattag tattagatgg taagttttaa atttatcaaa atcagaaagc
    25081 tgtaataaaa ttcttataat atgaatatct taattaagag atattgtaaa ttatgaggag
    25141 gcgctaatat ggaaggaaag gtaaagaagg cattcccggg tggaaataca tcacaaggct
    25201 catattcttt ttttgactat ataattcctg aaaatgtaaa aagagttttt tgtttaaagg
    25261 gtggacctgg agtaggaaaa tcctcactta tgaaaaaaat ttataaagag tttttgcata
    25321 gaggatatga tattgattta tatcattgtc cttctgaccc aagttcgttg gatggtttag
    25381 ttataaagaa attaggcgtg gtacttatgg atgcaactgc cccacatact atggacccaa
    25441 agctacctgg agctttggat gagataatta atttaggtga ttactggaat acagaagaat
    25501 tagaaaagaa taaacaagaa atatcagttt atgataaaga tattagtaat tcatttaaaa
    25561 gagcatttaa gtttttaaaa tcagcagaac caatctttag agacatagaa gaaaaatatt
    25621 ctgattgtat ggattatgga aaagtaaatt tggtcacaga agaatttata aaaaggttat
    25681 ttgatggagt taaaagcaca ggaaatctaa aaagagaaaa gcatttattt ggttcagcta
    25741 taactcctgt agggtgttta gattatacag aaaacatttt aaaagatgta aagaaagtct
    25801 attatttaga tggagaaata ggaactggga aaactacatt attaaacaaa gtatataaga
    25861 aagctattga aaaaggttta aatgtagaag tttatcatta tccacttatt ccagagaaaa
    25921 ttgaaactgt actattaact gatttagata taggcataac tataagtacg acttttaaag
    25981 aagaagatac aattgattta aatcagtttt taaatagaga aaaactactt aaatatgaag
    26041 aagatattaa atttgatgaa aaagttttag atgatttaat ttgttgggca gtactaaatc
    26101 ttaagagagc caagtctaaa catgatatta tagaaagtta ctatagtcct aatatgagat
    26161 ttgatgaagt agggaaatta agagataagt taattaaaag aattttaaaa tatgaggaaa
    26221 acttataaac gcatatgttt aaaattaacg ttagtaataa tataattttt agtaaattgg
    26281 tgtagctttg aggctatgcc attttacttt ttttgctaaa aaatgatatt aataatctcc
    26341 aaagccatta tttgtttttc ataattatta tttcctttcc taatttatgt ttttttataa
    26401 tatcacaaca atgtttattg aaagtttata taattttaag aacatctaaa aatgcatact
    26461 tgatattggt aaattagtga taggagttca atgtatgata ttatatacta aagatgaatt
    26521 ttaatatata actttgatat aataagcata tgtaaatgtt taatatttaa ttaaagaaaa
    26581 ttatcatata aaaataaatt tgtaaggagc aatacagtat gaaaaaacaa tatgtgttta
    26641 taatactagc aagtactatt ttaggtatac ttgttggaac catatttaga gattatagca
    26701 atgaagaaac agtttataca agcaaagaca agtcaataag aaaagaaata aaaatgacaa
    26761 gaaaatctat aaaagaatta accaaagaga aagacaagtt agataaagaa attaattcaa
    26821 tgaaacaaaa tcaaaaaaat tctgaagaaa tatcagaaat agaaacactt agagatagtc
    26881 tatcatattc agatgtaaag ggtagtggga taatattaag tatagatgct atagatgaaa
    26941 aaacagggaa tatagctaat agtatagact ataataagat tcttgtaaaa gttataaatg
    27001 aattaaaagt aaacggagga aaatacatag aaataaatgg tcaaagaatt aatcaatatt
    27061 cagaagttgt acttgcagga agccatataa atataaatgg agttcctgta gcacaaccat
    27121 atgaaataaa gtgcattgga gataaggaga aactatctaa ttatgtagat aaaggtagta
    27181 aatatttaga tagcatacaa accaattatc caatgaaaat aagcataaaa actgaagaaa
    27241 atatctctat gaaaaaaatg aatataccta ataaattaaa gtatataaag ggtgattaag
    27301 attatattag aggaaagcag tttgccaatg ttaataccag aaaaacttgc aaaaaaatat
    27361 aatgtatttc cagttgagtt tcaagatgat aatttagttg tagaaataga agatgaggat
    27421 atttatgctc tacaggactt gagattagct acaggtaaag aaattatatt gaagaaggag
    27481 aaaaaagaac ttataagtga aaagattgaa aaatattata atagctttga acttgatgag
    27541 gattatgcaa aaaaattatt tgaaaatctt ttagaaaaag ctataaaaga aaacgcaagt
    27601 gatatacata tagaaccatt taaagaacat ttaatagtga gaatgagggt agatggagaa
    27661 ctaaaggaag tatcaaagtt tttagttgat gtctatccat cattgtctac ggttataaaa
    27721 ttaaaggcat caatggatat aacagaaaaa aggcttcctc aggatggtag ggtggatata
    27781 aagttaggtg gaaatttaat tgacattaga gtttcatcaa taccaactgt ctatggtgaa
    27841 aaaatagttt tgaggatact taatagagat acttttttaa aagacaaatt agaacttggt
    27901 ttttcagagg aagctataaa atctataaat cgtataataa ataaaagagc aggaatatta
    27961 ttagtgacag gtcctacagg tagtggaaag actacaacag tatattcctt attaaatgat
    28021 ttaaaaggaa taaataaaaa tattatgact atagaaaatc ctgtagagta taagatggat
    28081 ggcataagtc agattcaagt aaattcaaaa gtagggttga gctttgatgt aggtcttaga
    28141 gcgatattaa gacaagaccc agatataata atggtcggag aaataagaga tgcagaaaca
    28201 gctaagatag cagtaagagc agctataaca ggtcatttag ttataagtac tttacatact
    28261 aatgatgcag tatcttccat agctagatta ctagaaatgc aaataccacc atatctttta
    28321 aatgcctctc ttataggtgt tatatctcaa aagttggtta gaaaagtctg caatcattgt
    28381 agccatgaga ttatgataaa agataatttt agtggagatg taaatactaa ggtagctgta
    28441 gggtgtgaaa aatgtaatga taaaggatac tttggaagaa cagcaattta tgaaatctta
    28501 gaaattaatg atgatataaa gacatgtata agaaatatgg aggattctag tactattaaa
    28561 gatgtggcac aaaataatgg aatgataaca tttgaagata gttgtaaaag actaataaat
    28621 gaaaaaataa cgacattaga agagtgttta gtagtaaatg aaatgattta actgaattgt
    28681 atagttaaac ttcgtttggt cagttgtctt tatttaatct attttaatta agatgtttga
    28741 gtataaatta tatttaagtg aaataaaatt aaatgtatta aattagaaaa tattaagttt
    28801 atttaattgt aattaatata atttggaagg tattatagta catgagatta tctaaaaata
    28861 tattttataa tttagaaaag ttttatttag tattaaatag aatcagtgat aaagaaatta
    28921 gagtaatttg caaagagata ggcatattgc tggaatctgg atgtgaaatt acaaaaatat
    28981 ttgaaatcat tgagagtcag tcaagtaaaa aagtaaagaa cttgcttagt atagtatcaa
    29041 atcatattca aaaaggtaac tcaatagcag aatcatttca aataacaggt atattttcta
    29101 aattctttat aagtatgata aaagcaggag aaactagtgg aaacttagat ataataatga
    29161 gtgatttatc taattattat gataaagaat acaaattaaa aatgaaaata ataactattt
    29221 caatatatcc aataatactt ataatattat ctatcttatc aatgttgttt atattcgtat
    29281 ttgtaatacc taattttcag gtggttttta ccaacaatgg aattgaacca cctttaataa
    29341 ctagagtttt aatgggaata tctacagtgg ttacaaataa cttagcttat ataatattta
    29401 gttttatatt gttttcaata ggaagcattt atttctttat aacaaatgat aatataaaaa
    29461 aattgattaa ttcgttaaaa ttcaaaatac cttttataaa aaaaattaat cagttagtag
    29521 ctacaacaag attttcaagg gcattgtccg ttttaattag cagtggaata caaattgtgg
    29581 aagctataga tatgtcagct agtgttatag acaacgaatt tatttatgaa aggttaaaga
    29641 tttccaataa ttatataaaa aaaggaaata gcataggtaa atctttgaat ttagcaaata
    29701 tttttccaaa attatttatt tcaatggcaa atacaggaga agaaagtggg aaactagata
    29761 aaagtctaaa tgtaataaat aaattttatg aaaatgaatt aaataacaaa atagagcaaa
    29821 taatgaaatt tatagaacct atgctaattg tatttatggg attaattatt ggtactttta
    29881 ttttagcaat ggttgttcct atgtttgatg caattactaa tttttagaga gaaaaataaa
    29941 ttttattaga tgaaactttt gaaaaggaga gaatatttaa ttgataaatt tgataaataa
    30001 aaaacgaaaa ggatttacac ttgttgaaat gattgtagta gtaactattt taggcgttat
    30061 atctagtata gcattagtta agtatagtaa ggttcaagaa agtgccaaat taaatgcaga
    30121 ctatacgaat gctgctaata tagtaaccgc agctagcatg gcaattaatg atgatgaaaa
    30181 gacaatagac tctctaagtg tagagacatt gaaggaaaag ggatacctaa atactgttcc
    30241 agttcctcag agtacatcag gtaaattcga acttgtcata aatgatagcg gaacagatat
    30301 aagcgtaaat ataaattcta agcaatttta tccaaaataa tattttatat tgattgctat
    30361 aagttttaaa attattttta tttaagaagg agatggctat aattaaaaaa attctaaact
    30421 tagaaataaa tgatgattat ataaggcttg tatttttaag aaaaggtcat aaaaaagttt
    30481 ttatagataa atcaattata aaagagatta actttgatat aaaaaatgat agtaattcga
    30541 ttgagaagat taaatatata gaagatgtag ttagatttgt aaaagaggag attggtaaat
    30601 caaaaatatt atataaaaat ttatacttta acattcaaat tcaggatata gtcattagaa
    30661 atgttgaagt tctaaatact aagaaaaaaa gagacatatt aagcatgatt gaatttgaaa
    30721 taacacaata cattccaata aacataaata actattcaat aaaatataaa gttataaatt
    30781 ctaaagatga taaattgaat atacaagtaa ttcttttccc aaagagtatt attcaattaa
    30841 ttaagaacat ttcagaaaat ctaaatatga atccaaagac tataaatata aattttgata
    30901 tattacaaaa gcttataaac ttaaatttaa tagagaattt ttcagaagaa ggggtattta
    30961 tagaatgtaa aaaaagtcag ttaattataa atataacaaa agataaaaaa ataagagaaa
    31021 catacttgct atcaaaagag ggaaaatcat atgagttcat aaaaagatta ttaagggatt
    31081 ttaagtatgt ttattactat ggtatagaaa attgttttat tgaagaatac tttaaatata
    31141 cagataaatc tattgaagta aatccattaa gattaaaaga taaagtaaaa gtagaaaata
    31201 attttgatgc agatttagaa gacaatagga taaattatat aaatagtata ggaatgatta
    31261 tttaagtgaa agtggataaa acaataaatt ttcttaatgg atttaaaaaa actaaaccat
    31321 gtaataaaat ctataaaata ataatagtct taattttaac gtgtatttta tttaaatcat
    31381 ttatgtatgt gaaatctata aatgaacttg aagattatat aagtcaaaat aagctagtgg
    31441 ataaatcaag acacacttta ccaacaacag ataaaactag agagaaattt tctaaaataa
    31501 aacttaatga aataaaaaaa atatatgaaa caattggaat aaatagtatt actaaaatat
    31561 caatagataa aaataagcta gagatggaag gtgtgtgtag tgatttagat gttttaaaca
    31621 atttaaggaa aataaagtac ataaaatatt attacataga cagtattgta agagatggag
    31681 aatattatag atttagaata aaaagttaga tgggtgatta tttttgagat taaataatag
    31741 agaaaaaaat ctacttaaaa tattgttaat attagttatt atattggcaa gtgatatagc
    31801 agtaagttat ttggaagaaa acctaactag tttgaagaat gaaaagcaaa atataataaa
    31861 taaaataaat aatcaagata attatcaaat aaaggataat aagcatatag atgataaata
    31921 tatagaaagt gttgaatcaa ataaaaatga agaattaaaa aataatttta ataactcatt
    31981 tttagataaa aaaacaaata aagatatagt gttattagta gaaaatgaac tgagagatat
    32041 tgtgaatgta gaatatatta atcaagaaag tagtttagat aatcacaata aaaaacaagt
    32101 taatttagaa ataaaagtta atggagaact tgcaaatgtg ttgaaacttg aaggtgtaat
    32161 gaagaagtta agactaaata ataaacttga aaagatagaa attaataaat taaaagtcaa
    32221 taattttgat gttcaaaata ttccaaatga atataaacaa gaaaaagaca atattgttga
    32281 atgtaaaatg acagttagag tcatataaca aaaaagaata gataaagtaa aaaactatta
    32341 tcaattaata aaattttata gaggaattag ttttacgata attcatttct acaagaggga
    32401 ttgttgatga ttttatcgaa atcctaatta aagttaatat attgtaccaa aatataagtg
    32461 aatttaatag aatatttact tatactatag attgattaat aaaaaaatgg tataataaca
    32521 aattgtactt taatttaata ataagggagt ggaagtcata ttgttaggga gaaatgagct
    32581 atgcccttgt ggaagtggca aaaaatacaa gaaatgttgt ttaaataaag atattgtttc
    32641 agaaagagca tgcagaaaaa ctgaactttc acaaaaacaa tatacagatt tatattcaaa
    32701 gttatatgat tattcaagac aggataagtt taaagaagaa tgtgaaaaag ctaaagaaat
    32761 gttttatata atgcaaaatg atagtgtaaa tgaaaaattt gaaagatttt ttaatactta
    32821 tttaatacaa gaccacataa tggaaaataa aaaggttatg acagttggtt ttcttgaaga
    32881 aaatggtgca aacctaagtc aatcagaagt atcaatatta aaaagtatgt ttgaatcata
    32941 cgtaagtatt tatgaggtaa aagaagtttc tgctggaaaa atagtattaa aggactgttt
    33001 aagtggaaat gaactttgta cagaagatgt aaaacttctg agaagtttta aaatcggaag
    33061 ttgtatgata gctagaatag ttgatattga aggaactaac atattgatag atataactat
    33121 aagtatatca aatgaggtta aagatataat tttaaaggat attatgaatc tattcaatca
    33181 atatcaagat gtatacaaag atatgaagtc gttcttaatt tatcatacac atatactata
    33241 caagtatata caacagcttt tagacccaag tatagctgaa tatttaaaga gagaaagaga
    33301 taaaaacgat acaaaagtta attcagaaaa tgaattagct gaagaagaat gtaaagtttt
    33361 agatactctt aaagaaaatg tagaaaaaga aaatatagat gcatgtatag agttttggaa
    33421 tgaatataag aaaaatcatg aaaatataaa aggagctgaa aatggttggg cagctgctgt
    33481 tgaatactat gttaaaaaag aagcaggaca acctataact caagttcaaa tatcaaagaa
    33541 atacgatata agctctagta cactaggaaa gagatacaag gatttaaaaa tatcttaatt
    33601 aattataaaa agctttaagt catagataaa gaatctatag tataatatat gtataatgtt
    33661 tagaaagagg tattaataat gagtcttagt ttaggaaatg caattgctgt atcaacgaac
    33721 tttataaaat ctaacccagc tgtacctgtg tacaacggag aagaaatata tgcagttatt
    33781 tatcatatag aaataggata taataatggt gtatcacctg ataccaaaca atatataata
    33841 atagactata aagagtttga tttatctatg gataaaaaaa atgaactaat aaatattgct
    33901 aaagagtatt gtaaatctaa aggtattgta gaaaacgaaa accttgaaat aaacctttta
    33961 gacaatgaag aagcagagaa cttcttagaa aaggtgttta ataatatgaa ggttataaga
    34021 ggtcttataa ctaaaaaagg ataaataaat ggactatata ggaatagaaa atataacacc
    34081 ttatgaaaat acatatgaat ttagtgtata tgaatatgat gatgaaatca ccttaggtag
    34141 tgaaaagtta tatgtatgtg aattaagggt tgtattgatt aaagttaatt ctctgtatgt
    34201 tgaaagattg cataaatcag ttgaagcaat ggtcttagta aaaaacttga aaaaagattt
    34261 agataaaaca cttgttgtaa acaaaataaa gaattttgtg ctagatgaga tttgggtaga
    34321 aaatctagta aaagagaata tagaagttat atttgtagaa agctaggaat aaccctagct
    34381 tttttatatt acaatctaag agaggaaaac aatatgttaa gagtatctaa tgtaaaactt
    34441 ggaattgata aagatatatc tttattaaaa gatgttatac ttaagaagct aaaaataaaa
    34501 gaaaaaaact tagtcaaata tagtatatat aaagaatcta tagatgctag aaaaaaagga
    34561 aaaatggaat ttgtttattc tgtagatgta gaagttaaag atgaaaataa aatacttaac
    34621 tcaaaaataa aagatgtaac aaaaataaag gaaatcagat acgtaaatgt acctgtgggt
    34681 gataaaaaat taaagaataa tcctttggta attggtagtg gaccagctgg tttgtttgca
    34741 ggtctgcttt tagctcaaat gggttatgaa ccaatcatac ttgaaagagg tcttgatgta
    34801 gacagtagaa ccaaagatat aagtgatttt tggacaacta gaaaattaaa aaataattca
    34861 aacgttcaat ttggagaagg tggagcagga actttttcgg atggtaaact tacaactaga
    34921 ataaaagata tacgatgtag aaaagtatta gaagagttag ttcagtttgg agcaccagat
    34981 gaaatattat actcacataa gccacatgtt ggaacagata tattaaaaaa tgtagttaaa
    35041 aatataagaa atgaaataat aagtttaggt ggtcaagtta gatttaattc aaaagtaaca
    35101 gatatagttg tatcaaatga ttctatacaa tctgtaataa taaatgatac tgaaaaaata
    35161 gatacagata caattatatt agctgttgga catagtgcaa gagatactta tgaaatgttg
    35221 tattcaagag gtataaaaat agttcaaaaa ccttttgcaa taggagctag agttgaacat
    35281 ccacaagatt taataaataa agcacagtac aaggattttt ataatcatcc taggttagga
    35341 gctgcagatt atagacttat agagcatgtt tcaaatggaa gaacagcata tactttttgt
    35401 atgtgtccag gaggtagtgt catagcatct gcatcaaatg attttgaaat agttacaaat
    35461 ggtatgagtg aacatgcaag agataaaata aatgcaaata gtgcattttt agtaaatgta
    35521 acgccagaag attttaagag tgataatcca ctagcaggtg ttcatttcca gcagaagtat
    35581 gaaaaattag cttttgaatt aggtggaaaa aattataatg caccagttca gcttgttgga
    35641 gattttataa acaataagac ttctttgaat ttaggaagtg ttgagccatc atatagacca
    35701 ggatatacac ttacaaattt aagtgaatgt ttgcctagct tcgtaacaga gactatgaga
    35761 gaagcgttta tatctcttga taaaaaattg aatgggtttg caatgtatga tgctgttttg
    35821 acgggagtag aaactaggtc atcatcacca ataagaatag taagagatga aaatacttta
    35881 gaatcagtga gtattaagaa tctatatcca tgtggtgaag gtgctggata tgcaggtggt
    35941 atagttacag cagcagttga tggtataaaa tgtgcagaaa aaataataca aaaatacacc
    36001 aataatacaa aaatttaaca taaacttaat gattttttaa tatttaacat gaatttaaca
    36061 ttatcactcc tataatttga taaaatacaa taggttataa aatttttaga tacagtaatc
    36121 tctttacaag gaggaaataa tttgaacatt ataataagtc taatgggtgg attaggtctg
    36181 ttcttatatg gtatgaacct tatgggagaa ggtcttcaaa aatctgctgg gacaaagcta
    36241 aaaaagataa taaaactact tactagtaac ctatttatgg gagttttggt aggtacagga
    36301 gttacagcag taatacaaag ttcaagtgct actactgtaa tggttgttgg tttcgtaaat
    36361 gcaggaataa tgactttaaa acaggcaata ggagttataa tgggtgctaa tatagggact
    36421 actgttacag cccaattggt atctttcgac ttaactggta tggccccagt agcattaggt
    36481 gtaggtatta tactttattt atttggaaat aagcctagaa taaaaaatat agccgaaata
    36541 cttataggtt ttggtatatt atttacaggt atggatttta tgaagatggc agtggagcca
    36601 ctaagagact atcaaggatt tactgactta cttgttacat ttggtagata tccactactt
    36661 ggattattat tgggatttgg gattacagca ataatacaaa gttcaagtgc atctatgggt
    36721 atgttggttg cactggcagc agaaggtcta gtgccattat cagcagcact gccaattctt
    36781 tatggccaaa atattggtac atgtgtaact tcactgttat caagtattgg tgctaacaaa
    36841 aatgcacgaa gagcagctat gatgcatcta atcttcaatg ttttaggaac agtgatattc
    36901 ttaatattct tgaataaacc agtagtaagc atggttacaa gttgggaccc aagcaatgtt
    36961 gcaagacaaa tagctaatac acatacattg tttaatataa tatctgtatt gatactttta
    37021 ccatttacaa atcttattat aaaactagct ataaagcttg ttcctgatag agcaggtgat
    37081 atagatgaag atgaaactaa aactattaaa tatatagatg atagaatgat agaaactcct
    37141 tctatagctc ttgcaaatac gataaaagaa gcacttcgta tgggtgagaa ggcgaaggaa
    37201 agcctaaatg catctatgga agctttagta gaacattcaa ctgaaaaaat agataagaca
    37261 tataggcgag agagattaat caatgattta cagaaagcta ttttaaatta tttacttaaa
    37321 ctttcaaaag cacctttaga tgatgattca agagaagttg tggatacttt gtttaataca
    37381 gtcaatgata tagaaagaat tggtgaccac gctaaaaata tagctgaatt atcgcaagtt
    37441 gcaatagatt caaatatttc tttctcagaa gaaggacaat ctgaacttga tgttatgtat
    37501 aatagagttg tttcagcata tacttatgca ttagaatcaa tgagaactga taatgttgat
    37561 ttagcttgta aggttataaa aattgaagaa caagttgata taatggagaa atcttgtaga
    37621 gctaatcaca tgtatagatt gaacaataat ctatgtagta tagaaaatgg agttatttat
    37681 ttagacgtaa taagtaattt agagagaata tctgaccatg ctgtcaatat agcacaacaa
    37741 gttatagcaa agagattagg aaatgattaa aaataattaa tttaaaataa tataaaattt
    37801 aaaatagagg ataaataaaa gagaaacttt aaatgatacg tttagagttt ctttttttgt
    37861 aaatttattt gactaaaatt tttaaattta tacatagtta tagcatttag aagaatcgat
    37921 ttcctgataa taaatgatga gatatgtatg attttgtggc aaacttttaa aaaaaacacc
    37981 tatattttgt gttataatct ctttaaaacg aatatgctga aaagagggat ttaaatggga
    38041 aaaataaatt ttgattatag taaagctaca gagttttttt gtcaaaacga aatagatgtc
    38101 atgcaaccat atgtagatgt tgcgcacgac atgttacata ataaaactgg tttaggaaat
    38161 acttttttag gatggataga ccttcctaaa aattatgata aagaagagtt tgacagaata
    38221 aaaaaatctg ctgagaagat aaaatcagac tcagatgttc ttttggtaat aggtatagga
    38281 ggctcttatt taggttcgag agctgctata gatatggtaa gtcattcttt taggaatgga
    38341 cttaaaaaag agcaaagaaa ggctccagaa gtatattttg tagggcataa tataagctca
    38401 acatatatta tggatttatt agatataatt gaaggaaagg atatttctgt aaatgtaata
    38461 tctaaatcag gaactactac agaaccagct ctggctttta gaatatttaa agattactta
    38521 gagaaaaagt atggtaagga agaagcaaga aagagaattt atgcaacaac agatgcaagt
    38581 aaaggtgcac ttagacagtt agctacagaa gaaggatatg agacttttgt aataccagat
    38641 gatgtaggtg gtcgtttttc tgtattgaca gcagttgggt tacttcctat tgctgctgct
    38701 ggacttgata ttgatgctat gatgaaaggc gcaaatgatg ctagagaagc atttcaaaat
    38761 cctgacttaa aaagtaatga tagttataga tatgctgttg ctagaacaat tttacataga
    38821 aaaggaaaag atgtagagtt attagtgaac tatgaacctc aactacatta tgtaagtgag
    38881 tggtggaaac agctttatgg agaaagtgaa ggaaaagaaa ataaaggttt attccctgct
    38941 tctgtagatt tctcaacaga tttacattct atgggtcaat acatacaaga tggaaaaagg
    39001 cttttatttg agacagtact taatgttgaa aactgtaaga gaaatataac tataagttca
    39061 gaggaagtag acttagatgg tttaaattat ttagcaggta agactgtaga ttttgtaaat
    39121 cataaagctt ttgaaggaac tctattggct catacagatg gaaaagtacc taatttagta
    39181 ataaatatac ctcaactaga tgagtataat tttggatatt tagtttattt ctttgagaag
    39241 gcttgtggaa taagtggata tttattggga gttaatccat ttgaccaacc aggagtagaa
    39301 gcttacaaga aaaatatgtt tgctttacta ggcaaaccag gttatgaaaa agaaaaggaa
    39361 gagctagaaa aaagacttaa ataattttaa atagttaagg tttaattaag attaaaataa
    39421 tataatgaaa tcatcaaaaa ggagtttact caaaatagat gatttcattt tttttatgaa
    39481 aaattgggaa aaatatattt tgataattta tattatatta agatagggag tggtatgaga
    39541 tgtcaagaag aaagaaaggg ataagtttag taatattagt tgctataata agttcaatac
    39601 ttagtagttt tctaacaata attttagtta aggacaattt agtaagtaaa tctacaggaa
    39661 gttctacgcc gatagttgta aatgatgatg gtaagagtca aaatatttat caagcagttg
    39721 cagaaaaagc tacgccatct gtcgtaggta ttacaacaac tagtgttgat actagtaata
    39781 tgtttgcaat accaactgaa acacaaggtg taggaactgg aatcatagtt gattcaaatg
    39841 gatatatact tactaattct catgttatat cagatggtca agctacaagt gtaaatgtat
    39901 tattcaatga tggttcaaca acttcaggta aagtagtatg gtttgaccaa caacttgatt
    39961 tagctatagt taaggttgat aaaactggac taacaccagc agaatttgct gatagtgaca
    40021 aagtaaaagt tggagatata tcaatagcaa taggtaatcc tcttggatta gattttcaaa
    40081 aaacagttac tcaagggata ataagtggat tagatagaac tatacaaaca gaaaaaacta
    40141 atatgactgg tttattacaa actgatgcaa gtataaatgc aggaaatagt ggaggaccat
    40201 tgttaaatca aaaaggtcaa gtaattggta taaacacagc aaaagcttct caagcagaag
    40261 gattaggatt tgcaatacca atcaatacag ctaagagtat agttgaagaa gtaatcaaaa
    40321 atggaaaata tgaaaaagta actttaggca taaaaggtac agatgtatca aattatgaag
    40381 cagcaacagg tacaaaatta agtactgata aaggtgttta tgttgcagaa gtaatatctg
    40441 gttcatcagc agaaaaagct ggagtaaagg taggggatat tataactaaa gtaggagata
    40501 cagatataac aggtatgaat gatttaaata aaaagttgta tactttctca aaaggtgcta
    40561 gtacaaaaat aacagtaaat agaggcggaa aagcagtaac aataaatgtg aatttctaaa
    40621 tatgaccaga aagatagaga ctacatggta aagtgtagtc tctattttta tttaattttt
    40681 atagtatata catctaaatt aattaaaatg atgtattgtt tatagtttat tgctatattt
    40741 tcttataaaa ataaatttaa taaatattta aatttaattt acaaaaacaa tataaatatc
    40801 ataataaaat aaaatttttt aatattacta aaaatatata gacaaaataa taacaaatat
    40861 agtattatgt aattgtagaa atgcttttca taggtatatt ctatgaagat gaattgcata
    40921 aaaatgaaaa aatggaatgg atattattta ggggtgaaaa atcatgaatc cattagttat
    40981 aaacttacaa aaatgtagca tacatgatgg accagggata agaagtacag tattttttaa
    41041 agggtgccca cttgaatgtg tttggtgtca taatccagag agtcaaacat atacaaaaca
    41101 agttttgtat aatgaagaaa gatgttcaaa atgtgaagca tgtataaata tatgccctca
    41161 taaggcaatt tacaaaggtg aaacaaaaat atgcttagac caagataagt gtgagttttg
    41221 tgaaacttgc ttagactatt gtgtaaacaa tgcaagagag attgttggtc aagagtattc
    41281 tgtaagagat ttggttaaag agatagagaa ggatagaatt ttctatgaag aatcaggtgg
    41341 tggagtaact ctatcaggtg gagaagtaat ggctcaagat atggacttca tttgtggagt
    41401 aataaacatg tgcaaatcaa agggaatcca tgtagcaatt gatacatgtg gctatgcaaa
    41461 gtcagaaaat tatgaaagag ttgctaaatg tgcagatttg tttttatatg atattaagct
    41521 tattgatgaa gataagcaca taaaatttac aggaaaatca aatgacttaa tactgaaaaa
    41581 tgttaagatt ttaagtgaat taggggctaa cataaatata agaataccat taattgtagg
    41641 agtaaatgta gatgatgaaa atttagaggt taaaaaaatg atagagtttt taaagccttt
    41701 gaatatacaa gcagtaagtc ttcttccata tcataatatt ggtaaacaca agtatgataa
    41761 aatttataaa aagtatgaag gagaagaact acaaagacca tcagaagaaa aattagagga
    41821 aataaaaaga ctgtttgaag caagtaattt caatactaag attggagggt aataaaatgg
    41881 caagaggaac ttttgagaga actaaaaaat taagagaaga aagtattaat gcagaaccac
    41941 acatatctat tgaaagagca gtacttatga cagaagctta taaaaaatat gagggaagtg
    42001 tagagattcc tgtacttcgt gctttatcat ttaagcatta tattgagaat agaactctta
    42061 gcataaatga tggagaatta atagtaggtg aaaaaggtga ttctcctaat ggagcaccaa
    42121 catatccaga aatttgttgt catacaatgg aagatttaga agtaatgcac aatagagata
    42181 taataaattt tagcgtatca gaagaagcta gaaaaataca taaagaagaa ataattcctt
    42241 tttggaaaaa gagacaaaca agagataaga taataaatgc aatgacacct gagtggttag
    42301 cagcatatga agcaggtatg tttacagagt ttatggaaca aagagctcca ggacatacag
    42361 tttgtggaga tacaatatat aaaaaaggat ttttagattt aaagaaagac atagaagcaa
    42421 gattaaaaga gctagatttt cttaatgatt tagatgcata taataaaaaa gcagatttag
    42481 aagctatggc aatagcttgt gatgcaatgg ttatattagg taaaagatat gctgagaaag
    42541 caagacaaat ggcagaagaa gaaacagatg aagctaagaa aaaagattta ttactaattg
    42601 ctgaaacttg tgatgttgta ccagctcaca aaccagaaac ttatcatcaa gctatacaaa
    42661 tgtattggtt tgttcatata ggggttacta ctgaacttaa tatatgggat gcttttactc
    42721 caggaagact tgaccaacat ctaaatcctt tctatgaaag agatgtagaa aatggaatac
    42781 tagatagaga tagagcacaa gaattacttg aatgtttatg ggttaaattt aacaatcagc
    42841 cagcaccacc aaaagttggt ataacattaa aagaaagtag cacatataca gattttgcaa
    42901 atataaacac tggtggaata aatccagatg gtcaagatgg tgttaatgaa gttagttata
    42961 taatacttga tgttatggat gaaatgaaat tgatacaacc tagttcaaat gttcaaataa
    43021 gtaaaaagac tcctcaaaaa ttcttaaaga gagcttgtga aatatcaaga aaaggatggg
    43081 gtcaaccagc attttataat actgaagcaa tagttcaaga gcttatggaa gctggaaaaa
    43141 ctatagaaga tgcaagactt ggtggaacca gtggttgtgt tgaaactgga tgttttggta
    43201 aagaagcata tgttctaact ggatatatga acattccaaa aatacttgaa ttaacattaa
    43261 ataatggata tgacccaatt agtaaaaaac aaataggtat tgaaactgga gaccctagaa
    43321 atttccaatc ttatgaggaa ctatttgaag catttaaaaa gcaattacat tatatgattg
    43381 acataaaaat agaaggaaat gctgtgatag aaaatatatg tgcaaaacac atgccttgtc
    43441 cacttatgtc tacaatagtt gatgattgta tagaaaaagg caaggattac caaagaggtg
    43501 gagcaagata caacactaga tatatacaag gtgttggtat aggaactata acagatagtt
    43561 taacagcaat aaaatataat gtatttgata agaaaaaatt cgatatggat acattgctta
    43621 aagcacttga tgctaatttt gaaggatatg aagctatact taatttagtt tcaaataaga
    43681 ctcctaaata tggaaatgat gatgattatg ctgatgaaat aatgcaagag atattcaatg
    43741 catattacaa tgaggtaact ggtagaccaa ctgtttgtgg tggagaatac agagtagata
    43801 tgttaccaac aacttgtcat atatactttg gagaaattat gggagcaagt ccaaatggta
    43861 gactttgtgc aaaaccagtt tcagagggaa tttcacctga aaaaggtgga gatactaatg
    43921 gacctacagc agttataaaa tcttgtgcta agatggacca cataaaaact ggtggaacat
    43981 tattaaatca aagatttgca ccatcagtag ttcaaggaga aaaagggtta gataatatgg
    44041 ctaatctagt aagagcatac tttaatatgg atggtcatca tatacaattt aatgtatttg
    44101 ataaaaatgt actgttagag gctcaaaaga atcctcagga ttataaagac ttaatagtta
    44161 gagttgcagg atatagtgac catttcaata atttaagtag aacactacaa gatgagataa
    44221 taggaagaac tgagcaaaca ttctaggttt gcttgtaaat aaaacttctt actatataaa
    44281 atcgtaactg atataataaa gtataaatgg tttgattcca tttttaaaat tttagattaa
    44341 ttatgagcta actttctaaa taggaagtta gctcgttaag tttaatgaaa aaatcgttaa
    44401 atttatattt agtaaatcat acacatatta gtaagtaata catacaaaag aataatttat
    44461 tatagttata ataaggttat attttataaa aataagataa ttttaagcag ttaaaataaa
    44521 gtcaatttag ctactttaaa ataaaactaa tagaaaaaag gagatttgat atgaaaactt
    44581 taggatttat tggttcaggg aatatgggaa gtgcaatgat aggtggcata gtacagtcag
    44641 gattagtaaa ggcagaagat ataactgtat cagatttaag ccaagttgcc ttagataaag
    44701 taaaagaaaa atttggagta aatactacaa ctgattcaaa ggtagctgtt acaaattctg
    44761 atgtggtaat agtagcactt aaacctaata tatgcgaaaa ggctttaact ccacttaaag
    44821 aattaataga tgcaaataaa ataatagtat ctatagcagc aggtaaaact atagaaagtc
    44881 tggaaagttt tataggttca gataaaaaaa tagtaagagt aatgcctaat actccagccc
    44941 ttgttggcga aggaatgtca gctctttgta aaaatagtaa tgtaacagat gaagagttaa
    45001 atatgattaa ggctctgttt gaatcttttg gtgaggcaga aatagtatca gaatatttaa
    45061 tggatacagt tacaggtatt agtggttctt ctcctgcata tgtatttatg tttatagaag
    45121 ctatggcaga tgcagcagta ttagctggaa tgccaagaca acaagcttat aaatttgcat
    45181 ctcaagcagt aatggggtca gctaagatgg ttcttgaaac tggaaaacat ccaggtgaat
    45241 taaaagatat ggtttgttct ccagcaggaa ctactataga agctgttaag gtattagaag
    45301 aagaaggctt tagagcagca gttataaaat ctattgttgc ttgtatagat aagtctaaag
    45361 atatgagcaa ataacagatg taattaagtt tactatatgg tttcttgaat tgttttttta
    45421 gtgcttattt atatctatac ataaatgtat ttggtataag taaaataaga gtaatttact
    45481 ataaaaagga atttaatgat tgagtgataa tttattactt aaatattaat aattaaatag
    45541 atatattttt aactatattt gagatatat
//
LOCUS       DAGSWI010000016        43687 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.016, whole genome shotgun sequence.
ACCESSION   DAGSWI010000016 DAGSWI010000000
VERSION     DAGSWI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.016"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            68..481
                     /locus_tag="MJF80_000811"
     CDS             68..481
                     /locus_tag="MJF80_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279485.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKYGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            549..965
                     /locus_tag="MJF80_000812"
     CDS             549..965
                     /locus_tag="MJF80_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279486.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLSTKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            966..1178
                     /locus_tag="MJF80_000813"
     CDS             966..1178
                     /locus_tag="MJF80_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0279487.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAVDIAKVFDTTVENLFEFIEEE"
     gene            1575..1745
                     /locus_tag="MJF80_000814"
     CDS             1575..1745
                     /locus_tag="MJF80_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279488.1"
                     /translation="MSALLYGVCNKPILISIVTNMQEFKIGVSMNYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            2288..3166
                     /locus_tag="MJF80_000815"
     CDS             2288..3166
                     /locus_tag="MJF80_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBZ0279489.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            3202..5097
                     /locus_tag="MJF80_000816"
     CDS             3202..5097
                     /locus_tag="MJF80_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0279490.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVVTALIIGVTKPTIRSENS
                     EVELLGA"
     gene            5365..7272
                     /locus_tag="MJF80_000817"
     CDS             5365..7272
                     /locus_tag="MJF80_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBZ0279491.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGIYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNVNNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            7262..7711
                     /locus_tag="MJF80_000818"
     CDS             7262..7711
                     /locus_tag="MJF80_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0279492.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASVVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            7811..8698
                     /locus_tag="MJF80_000819"
     CDS             7811..8698
                     /locus_tag="MJF80_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBZ0279493.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            9103..10551
                     /locus_tag="MJF80_000820"
     CDS             9103..10551
                     /locus_tag="MJF80_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBZ0279494.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERYNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            10577..12967
                     /locus_tag="MJF80_000821"
     CDS             10577..12967
                     /locus_tag="MJF80_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBZ0279495.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDYFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSCGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPTGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            12969..13640
                     /gene="pgmB"
                     /locus_tag="MJF80_000822"
     CDS             12969..13640
                     /gene="pgmB"
                     /locus_tag="MJF80_000822"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBZ0279496.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNCNTLLQAWSK
                     KINLD"
     gene            13679..15043
                     /locus_tag="MJF80_000823"
     CDS             13679..15043
                     /locus_tag="MJF80_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBZ0279497.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKKNSILN
                     NIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYFMP
                     IILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYVASVIP
                     ALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLASV
                     INLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCANL
                     AQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGAGI
                     AGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFGVE
                     KNVK"
     gene            15390..16232
                     /locus_tag="MJF80_000824"
     CDS             15390..16232
                     /locus_tag="MJF80_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBZ0279498.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            16377..18305
                     /locus_tag="MJF80_000825"
     CDS             16377..18305
                     /locus_tag="MJF80_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBZ0279499.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPDLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            18366..19793
                     /gene="ascB"
                     /locus_tag="MJF80_000826"
     CDS             18366..19793
                     /gene="ascB"
                     /locus_tag="MJF80_000826"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBZ0279500.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            20204..20980
                     /locus_tag="MJF80_000827"
     CDS             20204..20980
                     /locus_tag="MJF80_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBZ0279501.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            20996..21361
                     /locus_tag="MJF80_000828"
     CDS             20996..21361
                     /locus_tag="MJF80_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBZ0279502.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(21527..22276)
                     /locus_tag="MJF80_000829"
     CDS             complement(21527..22276)
                     /locus_tag="MJF80_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBZ0279503.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            22754..23179
                     /locus_tag="MJF80_000830"
     CDS             22754..23179
                     /locus_tag="MJF80_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0279504.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            23211..24398
                     /locus_tag="MJF80_000831"
     CDS             23211..24398
                     /locus_tag="MJF80_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBZ0279505.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            24590..24994
                     /locus_tag="MJF80_000832"
     CDS             24590..24994
                     /locus_tag="MJF80_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBZ0279506.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
     gene            25640..26500
                     /locus_tag="MJF80_000833"
     CDS             25640..26500
                     /locus_tag="MJF80_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBZ0279507.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGLNLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            26490..28361
                     /locus_tag="MJF80_000834"
     CDS             26490..28361
                     /locus_tag="MJF80_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBZ0279508.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASMLALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSITLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            28381..29835
                     /locus_tag="MJF80_000835"
     CDS             28381..29835
                     /locus_tag="MJF80_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBZ0279509.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            30860..32068
                     /locus_tag="MJF80_000836"
     CDS             30860..32068
                     /locus_tag="MJF80_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279510.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLI
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            32099..33478
                     /locus_tag="MJF80_000837"
     CDS             32099..33478
                     /locus_tag="MJF80_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0279511.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            33584..34774
                     /locus_tag="MJF80_000838"
     CDS             33584..34774
                     /locus_tag="MJF80_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0279512.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNIPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(34902..36230)
                     /locus_tag="MJF80_000839"
     CDS             complement(34902..36230)
                     /locus_tag="MJF80_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279513.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(36848..38062)
                     /locus_tag="MJF80_000840"
     CDS             complement(36848..38062)
                     /locus_tag="MJF80_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBZ0279514.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITNEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            38488..39204
                     /locus_tag="MJF80_000841"
     CDS             38488..39204
                     /locus_tag="MJF80_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBZ0279515.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRSDNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            39339..39965
                     /locus_tag="MJF80_000842"
     CDS             39339..39965
                     /locus_tag="MJF80_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBZ0279516.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(40178..41722)
                     /locus_tag="MJF80_000843"
     CDS             complement(40178..41722)
                     /locus_tag="MJF80_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBZ0279517.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(41748..42389)
                     /locus_tag="MJF80_000844"
     CDS             complement(41748..42389)
                     /locus_tag="MJF80_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBZ0279518.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            42659..42850
                     /locus_tag="MJF80_000845"
                     /pseudo
     CDS             42659..42850
                     /locus_tag="MJF80_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            43497..>43687
                     /locus_tag="MJF80_000846"
     CDS             43497..>43687
                     /locus_tag="MJF80_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0279519.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNL"
BASE COUNT        16894 a         4783 c         7422 g        14588 t
ORIGIN      
        1 tttggaaaag ttatatattt ataacataag gtgttataaa tatataactt ttaaggggtg
       61 attttatatg tctaaatcag agcttggtgt taaaacaaat cttgtttcag agttacttat
      121 gatatcctat ttttttatag actcattatt aaaaaagttt atttttaaat atggaaatct
      181 ttgtattact tttataattt tattcgcttt ttgggcatgt gacttcatgt ataataaaaa
      241 atataatgat ttagttgacg aggcgtcaaa acttatgtta ttaaaggttg atcatattgc
      301 atcaaaaata atgttatatt ctattttgct aatagctgtt ttcttttcag caaattatac
      361 aagaggtttg gatatatcaa atttggatat aggtgtaatt ctcttagcta ttttatttat
      421 acaatcttta tctaaattaa ttttgtatat acattttgat agaaagggaa tatgcaacta
      481 ggtgataata ttgataaaaa cgaaaattaa agagtataga gaatataatt tttaaggagt
      541 gattttatat gtctaaatca gaaactggtg taagatcaaa gcttatcctg gagttgcttg
      601 taataactta tttttttata gctgtattat ccactaagta tatttttaaa ggtccaagca
      661 ctgcttctct tccagggttg agttggttaa tagtttttgg atgttattca atttataata
      721 aaaaatataa tgatttagtt gatgaatcat ctgaatttat actatcaaag gttaataaga
      781 ttgcaataaa aataatgata tattctatag taataatatc tattttcttt gcaacacctt
      841 atacaagtaa tataaatata tcaaacctag atacaggtat aatcctcatt gcagttttat
      901 ttatacagtc tttatctaaa ttagtgttgt atatatactt tgataaaagg gggatatgta
      961 actaagtggc aatattgatg aaaacaaaaa ttaaagaata cagagaaaaa ctccttatga
     1021 cgcaaaatga acttgctaaa ttggttgggg taagacgtga gactattgta cacttagaaa
     1081 atgggaaata taatccttct ttgaagttag ctgtggatat cgctaaagta ttcgatacta
     1141 cagtagaaaa tttgtttgaa tttattgaag aagaatagtg gaaaatttat aaacatttat
     1201 aagatggata ataaaatata gttatttaat cattaagttt gttaaaaata tttatataat
     1261 ataacatgtg attaaggtca gattataatt gttttaagat aaaatgtaat aaaatttaat
     1321 ggagaaattt tataggtaga taaattttaa tatttataag attatgttaa ggaaacactt
     1381 aaaaattaga ttaaaattgt gggtatttaa atcaactaaa agatattgac tatttagtca
     1441 atatcttttt ttattaaaaa ggaatataca tataaatagg ttagattaaa aattgggata
     1501 aatatgattt atgtagcatc atttaattaa aaggagctat attagtattc attcatacaa
     1561 ttggataata tacaatgtca gccttgcttt atggtgtttg taataaacct attttaattt
     1621 ctattgtaac taatatgcaa gaatttaaga taggagtatc tatgaattat ggtgtattag
     1681 taattggtat atgctatata gcattaggaa taatttttaa attagataga aaaagattta
     1741 cataagataa caaaggctaa tttataaatt gctgcttaga aaaccaaatc actttattag
     1801 ctagagatga atttggaggg tgtcactttg gtgttttatg taaagtttat ggaagtaaaa
     1861 cattagataa tcgtgagtat gatttctata aatcatatat actattggaa tccaagaaaa
     1921 ggtattggcc aaatagctaa tatcttttta atttttttga ctctaatata gcattaacta
     1981 ttgcatgagt atctttaaat ttatagttaa tgtatcttaa ttgtttatta taaaatctag
     2041 gaaggctaaa gcataaaaca cttaaaaaga gaagatgtat atgagaaata taattaacta
     2101 tgatgcagac ttctaaaaat tatttagtga aaaggaactt agagtgtata ataaaagttc
     2161 cttttcacta aggaaaacga ttgttatttt ctgatcttca ttacactgat attatttaaa
     2221 tataaacaac aacaaaatta acaagaagca aattcaacct aaaatttaaa atatcgggag
     2281 gtacaatatg ttaataaata tgaaggaaat gctaaaagtg gcacaagaaa atcaatttgc
     2341 tgtaccagca tttaatatag gaagtgggca aatacttaaa gcagtagtac agagtgctaa
     2401 cgaaaaaaat gcaccagtaa tacttgcaat acacccaaat gagctaagtt ttctaggaga
     2461 tagctttgta gctagttgca tagaagaagc aaataagagt aaagtaccta tggtaataca
     2521 cttagatcat ggggaaaata aagaacaaat attaagggca ataagatgtg gatttacatc
     2581 agtaatgatt gatggctcac atttaccata tgaagagaat gtagctatat ctagggaaat
     2641 agtagaaata gcaaaaggat taaatgtgtc cgtagaagga gaacttggaa caataggaac
     2701 aacaggaaca tcaagtgaag gtggtactga tgaaataata tatacagatt caaaattagc
     2761 taaggatttt gttgaaaaaa caggagtaga cacactagct attgcaattg gtacagcaca
     2821 tggtatatat ccaaaaggtt ttaaaccaga gcttaaacta gatttattaa aagaaataag
     2881 agaagtagta gatatacctt tagttcttca tggtggttca tcaaatccag atgaagaaat
     2941 agcacaagca gttaaactag gagtttgtaa aataaatatt tcgagtgatg ttaagtcggc
     3001 atattataag aagtgcagag aactattaga acaaaaccca agcttatatg agccagatac
     3061 tatatatcca ccatgtataa aatctgcaag agaagttata gagtttaaaa tgaatctatt
     3121 caatgctata gataagttaa agtgctttta taaaaataaa aattaattat gaggtaacaa
     3181 attaaaagga ggggagaatt tatgataggt aaaatactac gaagcgattg tattgtacta
     3241 gataaacaat tacaaacaaa aaaagatgta attaattata tggcaaatca tttgtaccat
     3301 ggaaattata taaataataa agaaaagttt atagctgata ttaataaacg cgaaaaagaa
     3361 tcatcaacta atatgggtaa tttaatagct atacctcatg ctaagtcaaa tgtagtaaat
     3421 caacctgtaa tgatgtattt aagaacagat agagagatta cagataatga tggtcaaaaa
     3481 cttaggtcat tttttatgat agcagttcca gaatcaggtt cagatgaaca tcttaagatt
     3541 atatctaaac tagctacata tctaatggaa gaaaattttc ttaagcatct actaagtgca
     3601 gaaactccat acgaaatatt aagtatattt aataataaag ctgcagaatc aggagatttt
     3661 agtaataatc aaaataaagg tcttatagtt gggataacag gatgtccaac aggtatagct
     3721 catactttta tggcaaaaaa agcattagag gaagcaggtc aagaacttgg atatgaagtt
     3781 aaaatagaaa ctcatggagc tataggtgta gaaaataagt tgactaaaga agatataaaa
     3841 agagctgaat acgtaataat agcttgtgat aaagaagttc aactgagtag atttatagga
     3901 aagaaagtgc tacaaaagaa agtagtagat gctaagaatg tttcattagc taaaaattta
     3961 attataaacg ctgaaaatgg aatggctaaa gaaattaaac caggtggaaa gactttagaa
     4021 gattcaaaag aaatgcttca agggtcagag aaaaagggtg tttataagca cttaatgagt
     4081 ggtgtatctg aaatgattcc atttgtagtg ataggtggaa ttggtatagc catagctttt
     4141 atgtttggta tatttgcatc agacccaact catgaaacat acaaccctat agctggattc
     4201 tttggacaac ttggaggcga ttcagcattt aagttattta taccgatact tgctggtttt
     4261 atagctaaat caatagcagg aagacaagga tttgctcctg ctatgatagc tggttttatg
     4321 gcagttattg gaggttcagg ttttataggt ggtatggtag ctggttttgc aacaggatat
     4381 atagctttag gagtaatgaa gataacagaa aatttaccag atgtattaaa aggagtaaat
     4441 gctgtattga tatgtccact tttgattgca tttacgagtg gagctataat gttctttgta
     4501 gttaatacgc cagcatcatg gttaaatgaa gctttacaat cttggttaca aagtatgtct
     4561 ggaactaata aagtattatt aggtgcaata ttagcaggga tgatggcatc agatatggga
     4621 ggacctatta ataaaacagc ttcagctttt ggattggcta tgttttcaag taatatattt
     4681 gaaccatcag cagcattaat ggtaggagga atggtacctc cattagcgat atcattggct
     4741 atgttaatat tcaaaaataa gtttactaaa gaagaaagaa agtcatctct accaactgct
     4801 ataatggggg catctttcat aacagaaggc gcaataccgt ttgcagctgc agacccatta
     4861 agaatgattc cagcaaatat aattggttct tgtataggtg gggcaatatg tatgagttta
     4921 gatataactt taatggcacc acatggggga atatttgtaa taccatttgc atgtagtgca
     4981 ccagttgcat atatagcttg tatattgatt ggtacagtag taacagcatt gattataggg
     5041 gtaacaaagc ctactataag gagtgaaaat agtgaagttg aattacttgg tgcttaaatt
     5101 ataaataata gtaaaaataa ttaatggaat aacaacaagt atatagaata ataataagga
     5161 tatttagaat cagttaacta cttctaaata tccttaattt ttattttgaa attatattat
     5221 ctattatatc aatttagtaa ggtgtatcat aaattctttt atgatacaga agtatcttag
     5281 taattttaca tagttagtaa atatgttaaa atagtactta atataaacat agaaatatat
     5341 aaacataggt agggagggct aagtatgctt gtgtcgaggc aaattgaatt tcttaaattg
     5401 atactaaacg aaaataaata tagacctata aaatacttta aagataaatt aaatgtatct
     5461 gataaaactt tacaaaaaga tttgaagatg attgagaaat atttagaaac ttttaatata
     5521 aaaattgata taaaaagagg ctatggaata ttaatagaag atttagcaaa gaagaacata
     5581 gacttaatta atagtttaaa tatacaatcg aaggaaaata aatctttatc agtagagcag
     5641 agaagaatgg agattttaaa gtatttatta ctaaattcaa ataatataac ttcaataaat
     5701 cagttagcag ataaatatta tgtaagtaaa acttctatag taaatgattt taaatatata
     5761 gaggcatgga taaaagaata caatttaaaa cttaataaaa ctttagaagg aactaagatt
     5821 ataggtaaag aagtggatat aaggaaatcg attgccaaaa tgatggatga tttattagat
     5881 gaaaattatg aacaacatga tatatctgag ttaacaagat tagattctgc tacatttagt
     5941 gcattgataa atttatttga tattgattct attatatttg tagaaactat aattacagaa
     6001 ttagaataca atttaggata tactataaat cagccctact atataaattt aattacccat
     6061 atacttatat gtttaaagag aatcgaagaa ggtaatcaaa tagaatctca ggaagaaaga
     6121 gaaatttctg tagataattt ggacgagtta gtatataaaa atgtcactac acttatagat
     6181 aaaatagaaa atagatacag agtaaaaata acgaaagatg aaattatgta catatatatt
     6241 tttttagtat catctggatt tagtagcgaa aataatagta gttacaatga tgaatcagat
     6301 agattaagtg aaagtgaaaa agtttcatct ataatgatag agaatatgtc acgattttta
     6361 aatataaatc tcaaaaatga tgacttacta cagaaaagtc tagcatctca tgtaaggcct
     6421 atgttaaaca gattaagata tgatatccaa ataaaaaacc cgttgttagg agagatacaa
     6481 gagcgatttt cagatgtgct gggattatgt atgatgacta taaatattat gacagagtat
     6541 gataatctga agaatattag tatagatgaa gtctcgtatc ttgccacata ttttcaagcg
     6601 gcaatagaaa gaaatatgag tagtaaaaga gtaatagtag tttgtcatac aggatatgga
     6661 acatctcagc tccttgcagc cagattaaaa agagaatttc ctgaatggac tatagttgat
     6721 atagttccta tgcatcagtt gagtaagcga aatttagatg atgtagactt tgtaatatct
     6781 actgtaaact tagatttgaa agataaatta catatagttg tatcagtact attaatggaa
     6841 aatgatatta ataatataaa aaatgcttta ctttctaaac ctaaaaaaag tatgaactta
     6901 gatacaaact tattaggaat atatttagaa gataatatat attttaattt tgatgtagct
     6961 cagatggaaa gtttaatagg tgtgaattta gggagtaaat atgaatctat ttcattagga
     7021 aaagatttaa atatatatat acataagcaa agtaaattaa ataaagggat aatgaatgtc
     7081 aataatgtta ctagaacgat tgatatttat ttagaaatta gtaatcaaag ttttttaaag
     7141 aatattttaa tggaaatatc aaatttatat aggcaaaaga actatataaa ctatataata
     7201 gattgcaaga gcaaagagga tattaaaata ttatttaaga ataatctatt ggagaaaaca
     7261 aatggaaatt agagaaatat taacagaaga aagcattatt ttagatttga aatcaaccac
     7321 aaaagaaggc ataatacagg aattagtaga taaattatac gatacaggaa gtataacttt
     7381 aaaaagtaag tttataaagg cattaaaaca tagagaaaaa ataggctcta ctagtatagg
     7441 ttatcaaatg gccatgccac atggaaaaac aaaatatgta aagaaggcct ctgtagtaat
     7501 aggtaaatct aaaaaaggtg tagattggcc aagtatagat ggcttacctg taaaggtaat
     7561 aattatgttt gctatgccac aatctggagg caatgaagat atgatattaa tatctaaaat
     7621 agcttacatt ttaggtggta aaaatatagg tgaaagactt ttaaatgcac atacaaaaaa
     7681 agaaatatta gatatatttt atgaagtgta ggtattgtat aaattataaa tttgaggttt
     7741 ttaaaataaa atgtgtattc ttttaaatag tataaattaa tttttagaca ctagagagga
     7801 gaggatatat atggcaatat atttagaaat gcctaaatta atacatgaat ttccttttcg
     7861 tagtctatca aatgaaggag aagtattaac aacaccacac tggcataagg aaatagaaat
     7921 attacttata acagaaggtg tagtaaatct atttataaat gacaagccta tgcaattaaa
     7981 aaaaggcgag atagtgataa taaaaggagg agatattcat tatatactgc catcaccggg
     8041 aagtgaaaga ttagtatttc aatttgatat atcttttttt aatgatttaa ttttattaaa
     8101 tgaggaaaaa gaaagcttaa ttaatttatt cagtagtata aaaaaatgta gtagagattg
     8161 gtcagcaaaa ataagaaagt cagtgttcga tatattgata gatatctaca atgaagacct
     8221 atacaaaata gaaggatata gttatgcaat aaaaggtaaa atgtataatt taattcctat
     8281 attatataga cagattccta gagaaattga agaaaataca gtagaatatg aaataaactc
     8341 taaagagata ttagagagat tgaacaatat attcaaatat gtagaaaaaa attataaaca
     8401 acctataaaa ttagaagatg tgtcttatga agtaggattt agtgtatatt attttactaa
     8461 atttttcaaa aaaaatacag gaaaaacatt tataactttt ttaaatgaat atagattaga
     8521 aaaagcaaag tggatattac ttaataacaa tatatccatt gtagatttgg ttgaagaagt
     8581 tggttttgga agtacaaaaa ctttttacag agtatttaaa gaaaaaattg gagtctctcc
     8641 attagaattt aaaaaaagat ataatagctt ggaaaaaaaa gggagtgtct caaaatgaac
     8701 ttagttcatg aggcactttc ttttatatga aatcaaatat attttcagta tttcaacaat
     8761 gaaaattcca ttcatttata aagtctaaat tttagttctc aatacaacct ctatttcgaa
     8821 ataactcctt ttatatatat aaatatacag caatatatga gaataagata acaataatga
     8881 ggatataaaa atgtatttgc tatatatata attatataca tagatatcta atattgatat
     8941 ttaatatgaa agtattgaaa atagacaata ttaattatag tgagtaaaaa atatcattgg
     9001 atttttaaat tgaattattt aaaaggtata gtatgaaaaa aatcttataa ctaaatatga
     9061 gatagaaatt ataaaacttt atattgtgaa agaggtagat gcatgaaatt agggaaagat
     9121 tttttatggg gaggggcaat ttcagccaat caaagtgaag gattttattt agatggagga
     9181 aaaggtttat ctcagtttga taaactccca ttaaatgata agagattaaa agatgtatgt
     9241 ttagatgagg ataatatttt aaatgaaaat aatcaatact atccaagtca tatagggatt
     9301 aaattttgtg aaaattacaa agaagatata gcaatgttag cagaaatagg ttttaaatgt
     9361 tttagatttt ctatatcttg gtcaagaata ttcccaacag gagaagaaag tgaaccaaat
     9421 gaaaagggat tacaacttta tgataatata ataaaagaat taaaaaaata taatatagag
     9481 ccattaataa cgataagcca ctttgatata ccttattctc tagttgaaaa atattctggg
     9541 tgggctgata gaagagtggt taatttatat gtaaaatatg ctgaaatttt gatgaaaaga
     9601 tataaagatg atgtcaaata ctggattcct tttaacgaaa tgaatatgat aatgcatatt
     9661 ccatatatag gtgctgggtt aacttttata gctgatgaaa atcaacttca gaaaaaatat
     9721 caagcagcac accatcaatt attagctaat gcaaaaacta tagaaatagg aaggtatata
     9781 aataaagaat ttaagtttgg gtgtatgtta gctgctggaa aaacatatcc atacacatgt
     9841 aaaccagaag atgtatttgc aactttagag catgaaaggt ataatttgtt tttttctgat
     9901 gttcaagtta ggggtgtgta tccaagcttt atgaattatt atttaaaatc aaatcaaata
     9961 aaattaataa taaaagagga agatttatca acacttaagg ataatactgt agattttgta
    10021 tcttttagct attattctag tgcttgtagt tcagctagag ctgaaggttt agaaaaatca
    10081 aaggcaaacg gaccagaaac cataaaaaat ccatatcttc caaaatcaaa tagtgtatgg
    10141 caaaacgacc ctgtagggtt aagaataact atgaatcagt tatatgaaag gtatcagtta
    10201 cctttattta tagtagaaaa tggactgggt actcaagata ttgtgaatga agatggaaaa
    10261 ataattgatg attatcgtat agattacatg aaagaacata ttaaaaatat gattcttgga
    10321 gtaaatgaag atggaataga gcttatagga tatcttatgt ggggatgtat agatgtaacc
    10381 agtgtaagtg aaggaaagat gacaaaacga tatggcataa ttcatgttga ttctgatgac
    10441 tatggaagag gctcatttaa aagaactaaa aaacaaagtg ctttttggta taaaaaagta
    10501 atagaaacag atggagaaat tttatttaat gacatggaaa ctcatgcata attcatttta
    10561 agaaaggagt gttattatga gtttaatatt tactactaag gaccaactta tagagcaagg
    10621 gtttagtaag gatacaataa aatccaatga aacgctgttt acactagcta atggacattt
    10681 aggagttaga gggaatattg aagagtctga ttttaataat gagtatgtag ataatatggg
    10741 tacatatgta aatggatttt atgaagaatc tccaattaca tatggagaaa gcgcttatgg
    10801 ctatgcaaaa caaaatcaga ctatctgtaa gttacctaat gctcatatag taaatttctc
    10861 tattgaagga gattattttg atttatcaaa aggtattaca agtgaacatt ctaggatact
    10921 agatttaaat gaagggattc ttaaacgttc atttatatgg gaaaactctt gtggtagaaa
    10981 agttaaagta tctatagaaa gattagtatc acaagatata gaagaattaa tgatgatttc
    11041 atatacaata acaccattga attttaatgg aaaaataaaa tttaaaaata aaatggaaaa
    11101 tagtatggag aagtttaatt gtgaaaaagt agatgaccca agagtggcat ctataaataa
    11161 gaagcaattt aaaattaatg taaaaaaata taacaataag tattttatga atttagttac
    11221 agaaagaagt agattagggt tatggtgtgg gtttgattca tatattactt gtggaaatat
    11281 tacaaatgag acctataatg tttcagatga atgtattgag cgagaaatag aagtctatgc
    11341 caaaaaagat gaaaggttaa atttagaaat tgttgtaggc tatagtagag tatattctga
    11401 tttaaatcta aatgaagaac gtttaaaatc attagaatgt ctggagtcgt taaaatgttt
    11461 agaatcagaa gcacatttaa aatcattaga attggtttta agagaagcat gtaatattgg
    11521 atttgataaa gtcaaaaata ttcagagaaa gaaaatgaat gagttttgga caacctctga
    11581 tgtagagatt gaaggcgata aaactcttca actggggata aaaaccaatt tatttcatat
    11641 ttatcaatca gcaggaagag atggaaagac taatatatca gcaaaaggtc ttacaggtga
    11701 tggctatgag gggcattatt tctgggatac agaaatgtat atattaccat tttttatata
    11761 tactcaacca tccattgcta gagctttatt agattataga tactcaatct tagatgcatc
    11821 aagagaaaga gctagagaac ttggaataaa taaaggttgc ttatattcat ggagaactat
    11881 aaatggtaat gaatgcagtg catattatcc aacaggaaca gcacagtttc atataaatgc
    11941 agatatagct tatggtatta gaacgtattt tgaagcaact aatgatgaag aatttatgat
    12001 gcaaaatggc ttagaaattc ttatagaaac agcaagattt tgggttgaat ttggagatta
    12061 tatagaatca aaaaataata aattctgtat aaatggagta acaggaccag atgaatatac
    12121 agcaatagta aacaataatt actacactaa tttaatggct aagtataatt taatatatgc
    12181 agtacagatg gtagataaat ttaagcataa tttattttca aatgaagtat ttagaaaagt
    12241 taaattacaa gaatatgaag tgaaaaactg gataaatgcc gctcaaaata tgtatttacc
    12301 atatgatgaa gagaagaaaa taagcaaaca agacgattca tttttagaaa aatcagtatg
    12361 ggattttaaa aatacaccaa aagaaaaata tccattactt ctccattatc atccattaac
    12421 aatatataga tatcaagtaa ataaacaagc agatactgta ctttcagcgt tgcttttccc
    12481 tgatgagttt agcttagaac aaaagaagag agattttgaa tactatgaaa aaataactac
    12541 tcatgattca tctttgtcaa ggtcaatatt tggaatgatg gcaagtgaaa ttggaaatca
    12601 tgaaaaagct tataactatt ttatggacac agcattaatg gatattaata acctacaaaa
    12661 aaatacaagt gatggaatac atgctgctaa tatgggtgga acatggatga gtttagtata
    12721 tggatttgca ggaatgaaaa taaaaaatga taaattaagt tttgaaccaa ggatacctaa
    12781 acactggaaa aatattaaat ttaatatatt atttagagat aatttagttt ctgtaaatat
    12841 agaagaatat aaatcttatt ataagttaat caaaggtaga aatataacca tattacatga
    12901 taataaagaa ataaaattat caaagaacat aatagaagtt aataattgtt ttaaagggag
    12961 taattaatat gaaagcagtc ttatttgatc ttgatggagt tataacagat actgctaagt
    13021 atcattttga agcttggcat gagatagcaa aatcgctaga tatagaaata gatgaaactt
    13081 ttaatgaaaa attaaaaggc ataagcaggg aagaatcatt agataaaata ttgttatctg
    13141 ctaataaagt agatagttat actaaagagg aaaagttaaa ggtgtgtgaa gaaaaaaata
    13201 acatttatat aaagttaata aactcattaa catataaaga tatattacca gggataaatg
    13261 agcttataaa agaacttaaa aaaaataatg tgaaattggg aatagcatct gctagtaaga
    13321 atgcacctag gatattagaa tctttgggaa taaaagatga atttgattgt atagtaaatc
    13381 catctttatt aaaaagggga aaaccatatc ctgatatttt tattgaaggg agcaagattc
    13441 taaatttaaa acaatatgat tgtgtaggaa tagaagattc ttattcaggt attaaagcta
    13501 taaatgctgc taatatgaca tcaattggtg tggggaaaaa agaagtacta ggaaaatgtg
    13561 accatattgt agaagatact agcttattaa attgcaatac actattacaa gcttggagta
    13621 aaaagattaa tttggattaa aattatatta aaaattattt taaaagagag gataaggtat
    13681 gaagaataaa tatgaagaag tagctcaatt gataattgat ggccttggag gaaaggataa
    13741 tataattgac ttaactcatt gtatgacacg attaaggttc atcttaaaag atgaaaagaa
    13801 agtcaatgca gataaattaa aatctataga taaggtagtt ggagtaaact caacttcaac
    13861 tcaatatcag gttataatag gtaatgaggt aggtgttgta tttaatgcaa taataaataa
    13921 ggggataaat accacaggag atattaaaag cgatataaaa aataaaaaaa atagtatttt
    13981 aaataatata atagatacaa taactggttg tatgacacct atgataccag cccttacagc
    14041 atctgggatg ataaaagtta tattatctct ttgcaccact tttaattggc tatcatcaaa
    14101 ttcaagtaca tatagagtat tagatataat aggagatgta gcattttact ttatgcctat
    14161 aatacttgca ataaatgcag ctaaaaaatt taatgttaat acatcaatag cagttatagt
    14221 agtaggtgtg tttttacatc caaatttctc tgcatgggta tcaagtgggg accctatatc
    14281 atttatagga gtaccaattc aaggtgttat atatgtagca tctgttatcc ctgcactttt
    14341 aacagtatgg atgatgtcat atatagaaaa attcattgat aaattaacac ctagcatgtt
    14401 aaaaacaatt ttaaatccaa ctttagtatt attaataagt gcgccaatag cattaatagt
    14461 aattggacca ataggaaatt tcttaggaga gggattagct agtgtaataa atttactaca
    14521 aggaaggtta ggatttataa tggtttgttt attagcagct gcaatgccat ttatcgttat
    14581 gacagggatg catcatgcat tgactcctat attcatatca gcatttgcaa ctacaggaca
    14641 agaatcgcta atattaatat ctcaagtttg tgctaattta gcgcaaggtg gcgcgacatt
    14701 agctgtagca ttaagaagca aagatagtaa gatgaaacag ttagcatcag ctgcatggat
    14761 atcagctatc atgggtataa ctgaaccagc tctttatgga gttacattga aacttaaaaa
    14821 acctgctatg ttagcatcta taggagcagg aatcgcaggg ttatttgcag gaattactca
    14881 tgtgacttta tatgttccac aaaatagtat aatggcagta cttggattat caggggaaaa
    14941 ggggatttca aatatagtaa atggacttat agttatattt atagcattgg ttgctccatt
    15001 tattatgaca tatgtttttg gagttgaaaa aaatgttaaa taaagtacag ataatgtagt
    15061 agaggaagta taatattaac tatttacatt aatagaattt ttattattat tgactataac
    15121 ttgttttgta tttatattga tgcaaagaaa taagttaata ttaataagaa taattattag
    15181 aactgacaaa aagcaacata aaagattgat gaagtatatg gaaataaaaa gttgacaaaa
    15241 tatgtctgat gttttatcat attaattgga attaggtagt aattgtttaa gtacaagcta
    15301 agcttattca ttaataatag cagtatccta ggaaggagta tgcccattat tatggataag
    15361 ctttttttat gcttaaaagg aggataatca tgataattaa taagatatta aacaataatg
    15421 ttgtaatcac attagatgat aatgatgaag aagttatcgt tatgggtaaa ggaattggtt
    15481 atcaaaaatc aaaaggcaat ctaatagata aaactaaagt aaataaagta tttagaatat
    15541 caaataaaga aatatcaaac aaactacaag aattactcaa taatattcct atagaacaca
    15601 tgaaattatc aagtgaaatt atagaatacg cacaaataaa gttaaacaag aagctaaatg
    15661 aaagtattta catatcctta agtgaccata catattctgc aattcaaagg atgaaagaag
    15721 gaataaatgt aaaaaatgca atcctttggg aaattaaaag attttataaa gaagagtttg
    15781 aaattggaat gaaagcacta gatattattg aaaacaaaac aggtataaaa ttaccagaag
    15841 atgaggctgg atttatagcc tttcatatag tgaatgctca gttaagtgaa ggtcatactt
    15901 tggcatctga tattacgaag ctaatacaag aagtacttag catagttagg tatcatttta
    15961 ggatagaatt tcatgaagaa tcagtgtttt attatagatt tataatgcat ttaaagtttt
    16021 ttgcgcaaag attgctttta gatagcgcac atgaaggtga gacagacaaa gaattactaa
    16081 gcattattaa atctaaatat aataaagagt ttgaatgtgt agttaaaatt aaaaatttca
    16141 ttaaaaaaca atataactac atattaacag atgatgagct tatatattta acaatacatt
    16201 tagctaaagt agttaaagac tcaaatatgt aagtgaaata atttttcagc agtatattaa
    16261 gaatgattga aaggagtgat actaacaaaa ttatacttat ggttggatgg taacattgag
    16321 ttggccaaac cttcaaattt caaaaaatta atttaaacga aatatggagg agaaaaatgg
    16381 gaaaatacaa acaattagca caagagatag taaacaatgt tggtggaaaa gaaaatatta
    16441 gtggattagt acactgtatt acaagattac gttttacttt aaaagatgaa agtatagcaa
    16501 atgacaatgt attaaaaaac atggaaggta ttgttactgt aatgaaaagt ggaggacaat
    16561 atcaagtagt tataggaaat catgttgaag ctgtctataa agatgtagtt gaaataattg
    16621 gtttagatga ttcaagtaca agttcagaaa ctaaaaaatc aggtaatata ctagataaag
    16681 gtatagatat aatatcagga atttttcagc cagtgttagg gataatggca gcttgtggta
    16741 tgttgaaagg atttaatgca ttatttgtag caatgggatt atatagtgat gtatctggtg
    16801 gttatatggt aataaatgca gcgggagatg cattatttac attccttcct ttgttcttag
    16861 gatatacttc agctaagaag tttggattaa aaccaatgtt gggattagca ctaggagctg
    16921 cagtatgtta tcctgctata caaggtagca gtatatcagc aggagcagat gtgatgtaca
    16981 ctttatttaa aggaacaatg tttgcatcac cagtatatat tgatttcttt ggactaccaa
    17041 ttgtatcaat tgattatact ggaacagtcg taccaattat atttatagtg tattttgctt
    17101 ctaggtgtga aaaattattt aataagtgtg ttccagattt agttaagttt ttctttgtgc
    17161 caatgcttac attattaata acagtgcctg ttgcacttat agttataggt cctatagcaa
    17221 catttggttc tacacttatt tcacaaattg taatttcaat aagagatttt agtccactat
    17281 tggcaggtgc tatagtagga tttacttggc aaattcttgt tatttttggt atgcactggg
    17341 gatttattcc tgtatatata aacaatgtta tgactttagg atatgataat gttatgatgc
    17401 cattctttgc ctgtacattt gcaagttcgg cagttgtgtt ggcaatattt ttcaagacta
    17461 aagataaaaa actaaaagag atggcaatac caaactttat atctggtata tttggagtta
    17521 cagagccagc aatttatgga atattattgc ctttaaagaa accatttata ataagttgta
    17581 ttgctagtgg tattggaggc gctttctatg gattctttaa tcttagaaaa tttataactg
    17641 gaggaatggg aatatttgaa cttccagcaa tgattgaacc agatggggga atgggtaact
    17701 taatagtagc tctttctggt atagctatat caatggtcgt agcttttgta ttaactatga
    17761 ttttatataa agatgagaaa gtagaagagt ctaaaaaagt aagaaataag ggtaaagaag
    17821 aaataaaaga agtcaagtct actaaattag aaagagaaat agtaacaagt cctataaaag
    17881 gtgaagtatt aaaattaagt gatatagagg atgcagcatt tgcatctggt gtactaggtc
    17941 aaggagtggc aataatacct agtgatggaa aagtagttgc tccagtagat ggggtagtta
    18001 caaccttatt tcctagtcta catgctatag ggattctttc tgatacaggt gttgaagtgc
    18061 ttattcatat tggattaaat actattcaat tagagggaag aggttttgag gcatgtataa
    18121 aacaaggtga tagaattaca aaaggtcaaa ctatattaaa ctttgatgtt tatgcaataa
    18181 aagagttggg gtatagtact gtaactccaa ttgttattac aaattcatca caatttttag
    18241 atgtagtaga aacagaaagt aaaaatattg aattagaaga caatctaatt acagtcttat
    18301 tttagtaaag attgaggtat tagcataatc tagtgctttg gttataattt taggaggctt
    18361 tgactatgag ttttaagaat gattttttat ggggaggagc tactgcggcc aatcaatgtg
    18421 aaggtggata caatgaagac gaaagaggat tggcaaatgt agatgtatgt ccaacaggaa
    18481 aagatagaac agcagttatt acagggaaac taaaaatgtt tgattttgat gatgagcatt
    18541 attatccagc taaaactggt attgatatgt atcataatta taaagaagat ataaagttat
    18601 ttgctgaaat gggatttaaa gtatatagaa tgagcattgc atggagtcga atatttccaa
    18661 aaggtgatga agaaacacca aatgaaaagg gacttcaatt ttatgaaaat atatttaagg
    18721 agtgtaaaaa atataaaata gagcctttag tcactattac acactttgat tgtccaatgc
    18781 atttagttaa aaagtatggt gcatggcgta atcgaaaaat gataggtttc tatgagaaat
    18841 tatgcaatgt tatctttaga agatataaag gattagtgaa gtactggcta actttcaatg
    18901 aaataaatat gattttacat gctccattta tgggtgctgg tatttgcttt gaagaaggtg
    18961 aagatgttga aaagataaaa tatcaagctg cccatcatga gcttgtagct agtgcaattg
    19021 caactaaaat agcacatgaa gttgattcaa ataacttaat tggatgtatg tttgcagctg
    19081 gaagtgtata tccttatagt tgtaatccta atgatgtatg ggaagctaca aagttagata
    19141 gagaaaatta cttctttgtg gatgtacaat ctaaaggtaa atatcctaac tatgcgttaa
    19201 agtatatgga gcaaaaaggt attacaccag aaatggaacc tggtgatatt gaattattga
    19261 ataaatatac agtagatttt atttcatttt catattacaa tagccgttgt gttagaaccg
    19321 atgaaaatgc agatgatatg gctgaaggaa acatatttac atctgctaaa aatccatatc
    19381 tttcatacag tcaatgggga tggccaattg acccattagg tttgagaatt acactaaatc
    19441 atgtatatga tagatatgaa aagccactat ttattgtaga aaatggatta ggtgctaaag
    19501 atatagctga tgaaaatggt tatgtagaag atgattacag aattgattat ctaagagaac
    19561 atataaaagc tatgcatgat gcagtttcta tagatggggt cgatttatta ggttatacta
    19621 catgggcacc tattgattta gtaagtgctg gtacaggtga aatggaaaag cgttatggat
    19681 ttatatatgt agatagagac aatagtggta atggaagtct aagaagaatg aaaaagaaaa
    19741 gttttgaatg gtataagaaa gtaattgctt caaatggtga agatttatat tagacaagaa
    19801 atctatgtta aataagaaat ttgtattaaa taagaagagt ataaaaatta ataataagta
    19861 taatttttaa cttttttaac tatagaaaat caatacagaa ttattttaca ttgtatttat
    19921 gcaatagtat gattcacaaa taaaatttta gaaaataaaa ttgttaagaa gtatgggaag
    19981 taagtgcaat ttactttgta ataaaagatg aaaaaggcaa gcttaggttt taagcttgtc
    20041 ttttttatta tgtataaact atgatacttt aaaattctat gaaaaattta tttaatagag
    20101 aaattaaaaa tttaatggta atcaaattct ttaccaatga atataaaaaa tgtctacgtg
    20161 ttataatagt ttaaactaat aaacgaaagg tagatgaatg aatatgaaat accaacatat
    20221 aatagaagtt gacaaagaac tttgcattgg atgtgggctt tgtaaaaatg attgtccagt
    20281 aaacaatata attatagaaa ataaaaagtc agtaattaaa aaacaagatt gtctaatgtg
    20341 tggacattgt gcagcaatat gtcccacaaa agctattacc ttgacaggat ttgatgaacc
    20401 tcctatagaa cttacaaata agccaaagct agattcagat gaattactaa tggcaattaa
    20461 atctagaaga agtatacgta aatttaaaga taaagaagtt tcttcagaaa ttataaagca
    20521 aattattgag gcaggtaggt atacccctag tgctaaaaat tcacaagatg tatcatatat
    20581 tgttttagac aataaaaaat caatttatga aaacgaagca gtgaaatttt ttagaaagat
    20641 aaaacctatt gcaaatatag ctattaaata ttcaaaagaa gttgagattg acgataactt
    20701 tttctttaag catgctccta ttgcaataat gataattaca aaagataaga ttagtggttc
    20761 acttgcagca tcaaatatgg cattaatggc agagtcttat ggcctaggtg ttttatttag
    20821 tggatatttt tcagatgtag caaataattc gccaaagtta aaaaagcttt taggtctaaa
    20881 acgcagtaat catgttctta caacattagt aattggatac ccagatgtta aatatagacg
    20941 tactgcacag aaagaagtag caactgttag atacttataa gagagggtgg attttatgga
    21001 aaaagaatgt caacaaagaa tgcaagaaat aacgtctaaa tttaaagaat gtcgcaaagc
    21061 tatctctgct atgggtgatg agactagaca attgatttta attgctctct tagagagtga
    21121 ttttaatggt atacgagtgg gagaaatcac aaaaaaaaca catctttctc gtcctgctgt
    21181 atctcatcat cttaagatat taaaagaagc agagattgtt aatttacgta gagaaggaac
    21241 gaaaaattat tattatcttg attcaaaaaa atcacaatgg aagtcactaa cagagctaat
    21301 aaatcttata tttgttggaa tacaacatat tagtgaaaat gatataagaa aaggtgatta
    21361 aattatactt gatttaatac aaaaagattg atttaggaac ttaaaagttc actttgagac
    21421 actatctttt gaactatacc catcttagga gacagttaaa atatgtaaaa ctgtataaaa
    21481 caatgggtat agttcatttt ataatggttt aaatattaag aatatattat tctaaatagt
    21541 atctttctct gttttgagtt ttatttttat actcaatagc ttcagtgggg caaccacaaa
    21601 tacatgccat acaatgagta cagttatttt tccaaactgg ttttttgttt tttaaattaa
    21661 tattatttaa aggacatagc tctacacatt ttccacagcc aatgcattta tcagtataat
    21721 gaaatccttt ggcactaata attgtcttat aaaaaactgt atttattata ccacttttaa
    21781 ttgtacctcc taagccacta ggagtaatag tttcaaattc attttcattt cgtatatcag
    21841 aaataatcct atatatttgt ttatctgctt ctttaatcat ttgttttgat gtttctttgt
    21901 ctgttgtaga aaataaagct atataattct ctggcatttt aatttctgcc atacctttta
    21961 attgccattc cttatattta catagttttt gaatatagtt tatggatttt gctgtatctc
    22021 ccccacaagt cattacaaag taaactctgt tgcttcctag aaattttgtt tcttttataa
    22081 aatccgtaac cactattggc aatctccatg cataagtagg acatacaaat atgaatggtt
    22141 tatctgctga tattaattca tctgtttttt cctcttttat aagttgattt agtgatatca
    22201 gttcatcatt taattcttgt gcaattttac gagccacata tcggctatta ccagttccgc
    22261 taaaatataa aatcataata atatctccct aactattttt tattttaact cttaattttt
    22321 tatttattat agtaatttat tgatatattc tattcaaatg tatgtaaaat ttagtagtta
    22381 agaaaattat aacacattag taatttttat acttattggt aaagaatttg attaccatta
    22441 aatttagcaa caagacatca actggacttg tatatgagaa gggataataa tttgcttatg
    22501 aaagattata aatataaagg ttattttaaa ggtgtagaaa tatttgataa tgtaaatgac
    22561 cttaacttaa atactttaac aacagttagt agatttgtgc ttgttttttt gtttacgata
    22621 acttaaggaa atatgttacc tgaataataa atttatataa taaaagtata taaatatata
    22681 tatgaatgtt aagattagtt gatagaattt aaataataaa tccaatatta taaacatagg
    22741 aggtatgaaa aatatgatta atgagaattt aaaaagtcta agaaaaatca accagtatac
    22801 acaagaagaa ttagctgaaa aacttaatgt ttctagacaa tcaatagcaa aatgggaaag
    22861 tggagaatct attcctgata tatgtagttg cattaaactt gcaaaattgt acaatgttaa
    22921 actagatgac ttagttaatc attcggaaga aaaaacaggt attatagtac ctcctaaagg
    22981 aaagtttttc tttggagcag ttgtagttgg tgaacgtggt cagattgtca ttccaaaaga
    23041 agcaagagaa gtatttaata taaatgcagg agataaatta ttggtacttg gagatgagga
    23101 aagaggatta ggaattgttc atcaacgtga tttgataaat tttataggtc atgtgggagt
    23161 agtacgtaaa gaagaatgat ttaaaaaaat tataaaagga ggtatgaaat atgagtaaaa
    23221 tagtattttt tagcattcct gcatatggtc atactaatcc tactattgaa gtagttcgtg
    23281 aattagtgga tagaggaaat gaggtattgt attattcttt taatgaattt aaagataaaa
    23341 ttgaaggtgc tggagcaaaa tttatttgtt gtgataaata ccttccagaa ttactccctg
    23401 gagatgagaa aaaaattggt aaggattttc ctggattaat tgagatgata gtagatacta
    23461 ctatttcttt agatgaaaaa gtttgtagag aacttaaaga ttttaatcca gattgcattg
    23521 tatcagattc attaagtttt tggggaaagt tatttgcaca aaaacttaat attccatatg
    23581 tatgctctac aacaactttt gcatttaata aacatacagc aaagttgatg aaacaaaact
    23641 ttatagaaat tatacgtatg atttttggtg taagacgcat taataaaaaa ataaagcttt
    23701 tacaaagaaa agggtatgaa gtaaaaaatt ttatttctat agtttctaat gataatgata
    23761 cggataccat tgtatataca tcaaaagaat ttcaaccaat ggtagaaacc ttctctagta
    23821 agtattcatt tgtaggtcca tcagtatcaa agttgattat tgaacctaaa gagagaaaaa
    23881 gaaagctaat atatatttcg cttggtacaa ttaataataa aaatgttagc ttttataaaa
    23941 actgtataag tgcatttaaa gattctgatg ttgatgtgat tatgtctgtt ggtagaagta
    24001 ctgatattaa aagtttagga aatataccaa ataattttga ggtcaaaaat agtgttgaac
    24061 agattgctat acttcaaaaa actgatgtgt ttgttactca ttctgggatg aatagtgtta
    24121 atgaaagttt atattatgga gtacctatgg tattatttcc tcaacattcg gagcagagaa
    24181 tggtagctaa gagagttgtt catttgggag caggaattat gttaaaagag gataagtcag
    24241 aaagtattaa aaaagcagta tttcaagtta taaatgacaa tgaatataaa gaaaatgcaa
    24301 ctaaactatc aaaaagcttt tataatgcag gaggctcaaa aaaagcagca gatgtaatat
    24361 tacagattat atgtaatagt agacaagttg acatataata gatttgttaa aaatatatat
    24421 acatacatgt aatttaatca aagtactgac agtggggtct tttaacctac cagttggggc
    24481 atatatattg atgctaaata tgagggatgt tatattttaa taaaaaagat aacatccttt
    24541 ttttagtgca ttatatcgag ttttaaataa taaatgaagg aggatcaata tgaaaaaata
    24601 taaagttatt tttatagtat taattatagg tattataagt gtaacagggt ttgttgtata
    24661 taaaaactgc attaaaccat ggaaagatgc tgaaacatat attgataagt atatggtaaa
    24721 acaagatatt tcaaaagata atataaaaag tattacaaaa gaaaaagcca agaaggcaag
    24781 ttacgaagga atactataca aagtgtatta caaagatgac cctcgatatg agtatcaata
    24841 tttttatagt gataattatt atgctctata tgtaaataaa gtaatgttac aaatttatga
    24901 tatggaaaat aataataaac ttttaagaag taatgaggaa ttaaaaagtg taaagtatcc
    24961 accaatatat cttaaatata agccagataa atgatttatg acaaaataga atgattttta
    25021 ttaaaattta tttaaaaata tcactagagc gtttaaataa aactaaattt aagtatttat
    25081 atattagtat atcttactag tgtttaaata aaactaaatt taagtattta tatattagta
    25141 tatcttacta gcatttaaat gaaactaaat ttgaagcttt acctattaat atatcttaat
    25201 agtgattaaa taaaactaaa cttaagactt tatatgttaa tatatcttaa gaatttattt
    25261 gttaacatac agttcattat atttaaaagc attactatga taattcataa tagtaaaata
    25321 gataatggtt cagacaatat tataaatatt agtgacggta aaatagtatc aatttagtat
    25381 ataaaccttg tcagataatc gcataacaga gcttgtagtt atagaataaa aaaatatcta
    25441 ggaaaccgtt gacagattta tatggatagt ataacatata gaatatgaaa ttaaacaaaa
    25501 aagaatttta attatggcga cgtagtcata attttgagta tgttactgat tagcaggcag
    25561 aactcaagta tatatgtgaa aaatagaggg atactctata catatatgct tgagttcttt
    25621 ttattgaagg gaggaatata tgaaaataat tcagatttta aataataatg tagccttagt
    25681 aaaaagaggt aaaaatgaaa ttattgtatt tgttaaagga attggcttta aaaaaagagt
    25741 gggacaagta ataaatgaaa atgaaataga gagaacatat atactagatt catacgatat
    25801 gctagaacac tttagttatc tactacaaaa ctcagactcc aataatatag ttcttattaa
    25861 tgaaataatt gaatatggtg agaaagcttt aaatataaag tcaagtgatt atctaagctt
    25921 aaccttacta gaccatttag agtttttact taaaagatgt gaaaagaatc aatttataaa
    25981 aagtcctttg gtatggaatg ttaaaagatt ttatccaaag cattttgaaa ttggaatgca
    26041 tgcactaaaa ttaattggtg atagtaaggg acttaatctt cctgatgatg aggcagtatc
    26101 aatagcactt cattttgtaa atatggaggt aaataaagag tctcatgata gtacaattgt
    26161 agagttgaga actttagcag atatagtttc tataataaaa tatcatttta atgtagagct
    26221 agatgaaact tcaacaaatt atatgagatt tactactcat ctacagtact ttatccaaag
    26281 aattatgtcg gatgaagctt caatagatga agataattct atagatttat ataaacaagt
    26341 aagtcaactt tatccaaagt catttggaag tgttcaaaag ataagaaaat atataaaatc
    26401 acaatttaat gtcgatatat ctataagtga agaaacttat ttaatgttgc atataaatag
    26461 ggtgactcaa agactggagg caaaatcaga tgaaatataa agatttaaat gaaaatataa
    26521 taaaacttat agggggaaaa gaaaatataa agtcggtagc gcattgtgtt actcgtttaa
    26581 gatttacttt aaatgatagg agtatcgctc aaacagaaga aattaaaaaa ttaaaaggag
    26641 taattgatgt agtttctaat gatgttgctt atcaaattat aataggtact catgttgtag
    26701 atgtatataa cgagtttatg tctatgatag gtttaagttc aagtgaatca agtataaaaa
    26761 gtgaaagaaa aaagggaatt agaggtatca aaggtatatt taattccatc tttgtggtag
    26821 tctcagagac tatgacttca gtaattgaag tactacttgc agctggtata cttgcaagta
    26881 tgttagcact tttaaatatg tctggcatac ttcagagtga cagtcctaca tatcaaatat
    26941 tagatacatt aagaagtgct acatttcact tcttaccagt attaattgca atagcatcag
    27001 caaaaagact taatgtaaat ccctatatag cagtagtgtt agcagttacg ttattatctt
    27061 catcaataga tggtgtagaa ggattaagtt tatttggtat aaatttacag gctacaactt
    27121 atgcaaacag ttttattcca atacttttag gagtatgggc aatgggaatt ataattgaag
    27181 gtttgaaaaa aatattacct aaagcattac actatttttt agttccagta ttatctttaa
    27241 taataactct atcaattaca ttaactgtat ttggacctat tggtatgtgg attggtgatg
    27301 gattaaacta tgcatgtatg tttttaattg atacattggg taattggagt gttgttgcac
    27361 tgtatgcagc tttacaacca ctcttaatag tctttggtgc agcaaacttt actatgccta
    27421 tatctttaaa ttctgtgaca acattggggt atgacccaat ttttatgggc gctgcaacaa
    27481 tatctgatat tgcagtttgt ggtgcaatgt ttggatattt cttaaaaact aggaataaag
    27541 aacaaaaaca attatttggt acagtaagtt ttagtgcact tatgggagtt acagaacctt
    27601 cagtgtttgg tgtgtttatg aaatatagaa gaccatttgt agcagtaatg ataggtggag
    27661 gtataggtgg tttaatagca ggtttagctc aagttaaaac atatggaatg gtttggggat
    27721 tagctgcatt accaacatat cttgttggag gagaggtatc taactttaca tttatgataa
    27781 taagtgtagt agtatcattt gtagcagcaa cagcagcttc ctatttacta ggaatacctt
    27841 cagatgaaaa agaagaagtt aagggtgata atgaaaaaga attagaacta ataatcaacg
    27901 agaacaattc aagaactatt agtattggaa atgttgcaaa aggtcaggta attgcacttg
    27961 gggaagttaa tgacaaagca ttttcatctg gagcattagg taaaggggtt ggagttatac
    28021 ctagtgaatc aaaatcaact gttatatctc ctgtagatgg aactataact atagtatttc
    28081 caactaaaca tgcatatggt atagtgactg atgaaggatt agaaatatta attcatattg
    28141 ggatagatac tgtaaactta gatggaaaat tctttgaaag tcttgtaaca caaaatcaaa
    28201 gggttaaaaa aggagatgct ttagcagtat ttgaatctga taaaattata gaggaaggct
    28261 ttgattctac tataataact gtagttacaa atactagcga ttatttagat gtaatctcat
    28321 caaatgatga atcagaagaa ttattaactg taatagtata aattttagga gggaaaattc
    28381 atgaatatta aagatacatt tttctgggga ggaagtattg cagcccatca atgtgaaggt
    28441 tcatgggata gtgataacaa aggacctgca attatggatt ttgtaactaa gggctcttat
    28501 gaaacgccaa gagtaattac tgataaaata gaagaaaaat tggattatcc atctcataat
    28561 ggaatagatt tttataatag atataaagaa gatatagctt tatttaaaga aatgggattt
    28621 agtgcattaa gaatatctat agattggtca agaatttttc caaatggaga tgatgaaaat
    28681 cctaatgaat tagggattaa atactatgaa ggtctgatag atacattaat agaaaataat
    28741 atagaaccta ttgtaacact atatcatttt gaattaccta tgaatttagt acataaatat
    28801 ggttcatgga ataacagaaa acttatagat ttgtatttaa agtacagtga gactgtaata
    28861 agaagatttg atgataaggt aaagtactgg gttacattta atgagatgaa ccatattgac
    28921 cctcaaacag agcactcgga tatttttact tatatgatag caggtattaa gtatagtgaa
    28981 atagaaaata aggttcaaac attggcaaat gtagggtata atatgacttt ggcgggtgta
    29041 aaggttgcaa agttggctag agaaatcaat cccaacaata aagttggatg tgtatttgga
    29101 ctaaatccaa tttatccata caattgtaat cctagtaatg tgttaaatgc atttttagat
    29161 aatgaccgtg attattatca aattgatgca atgtgcaatg ggaagtttcc acaatataaa
    29221 ttaaaagaat atgaaaaaaa taatataact attgaagtga ctgatgaaga caaaaaagca
    29281 tttaaagaag gtaagttaga ttttatagga cttaattatt atttttcaag tgtatctact
    29341 ccaaaagaaa atttatcagg tgataaatct ttatttggtg ggattcaaaa tccatatttg
    29401 gaacaaagta agtgggggtg ggcaatagac cctataggtt taagatttac cttgaactat
    29461 atttacagaa agtatcaatt acctatctta attacagaaa atggtttagg ggcatatgat
    29521 aaaatcgaag aagatggaac tataaatgat gattatagaa ttgaatattt aagtaagcat
    29581 ctatctcaaa tgaagtctgc aattgaagag gataatgttg aatgttttgg atatttgatg
    29641 tggggaccaa ttgatttagt cagtgcaact acaggtcaga tgagtaagag atatggattt
    29701 atctatgttg acttagatga tgaaggcaaa ggaagtttta agagatataa gaaaaaatca
    29761 tttgattggt acaaagaagt aataaaatct aatggagaag ttttagattt ttctacggat
    29821 atagtagtag ggtaggaata agataaatag aagttaggaa ctaatgttaa ttagtgtcta
    29881 gtaaagcatt taaaatagag tatagggtct ttaaagtaaa gtgtgtattc ttcttaaaag
    29941 taatggcaac taaaattact ataaaaagga gaatacacat tttttatgtt tatacttaag
    30001 taaatattta gacatattca tagtataatt tactcattat ataaaaatag tatgatttac
    30061 tcactatata actttataaa ttttctggaa aaatgtcaga atatattgac aaagaaaaga
    30121 tttaaaatta taatctatat taagaatagt ttgacaatat aaaaacaagt atttttataa
    30181 atagtgaaat tatttttatt gtaaatttaa gaataattta ggactactat aaagataaag
    30241 gtatagatgt cattatatga taaatcatac ttgtatagaa agaattttaa agtatggctt
    30301 atcattgatt taatataata aataattttt gtttctagta atagttccta ggaataagat
    30361 atctaaattc gtttagaaat agtctaattt acaaaggagg ggatgcacta atagataaaa
    30421 tttctatata aagaagactt aaagtgaagt atattctgat aatattggtt taatgggtat
    30481 attattattt cgttttagat attttaagaa taatttaaga cgtttgcaaa aatgtattct
    30541 atatctaata tttaaataaa tataagtctt ccaacatgta tactaatata gagagtagtt
    30601 gaagtacaat tctaactcgt aaaattatgt agaaagtaaa aatcaaaaag aaatctagta
    30661 agttgataac tatagttaat atttagagag ttaactctag ttatatttat ttagatattt
    30721 taggaataat ttaggacgca tataatatca tcaaaaaata tcaagctata ccatatgtag
    30781 ttaagaataa ctgcataata tacattttat aattattgtt ttattgataa aaaattatta
    30841 tatttaggag gaaacaacaa tggaaaattt ttggaatcca cttacttgct ttactgtatg
    30901 tgtaatgatt tatgctttta gtgagttttt atctaagaaa acaaaaggtg caatttcaac
    30961 attgttattt gtatgtatta tatttttaat aggattttgg acaaatattt taccaaaaga
    31021 cattacagga gcatcaggga tattagctgc aatgagcaac tttggtatag ctttattaat
    31081 aactaactta ggaacactta taaatcttga agacttatgt catgaatgga aaacggttgt
    31141 aattgcatta gtaggtattg taggaatagg ggttggctgt ttaacagtgg gtagtatgtt
    31201 atttggtaga gagtatgctt taatagcagc acctccaata gcgggttcta ctgttgctgg
    31261 tattatcgta acaactgttg cagaagctgc aaatagacct gaacttgctg catttgtaat
    31321 tctagtatta tcatttcaaa agttctttgg cattccgata actacatatt gtataaataa
    31381 agaattaaaa tttaaaagag ataatgggga ttttttgaaa gatgagtcaa aaagtaattt
    31441 taaacttcca agtatgagaa tttttaaaga agatttaggt aaaaaatcat ctactctagt
    31501 gtatcttgca aagctttcat ttgttgctat gattggtaat tttgtaggat tggctacttt
    31561 aataccagga tttacccctg caaactatta tttaaatcct aatatagcat gtttgttagt
    31621 agggttgatt tttacacgaa taggtttttt agaaaaagga attttagata aatctcaatc
    31681 taatggaatt ataatgtttg gttgtatgtt gatgttacct ggaggacttg ctaaggtaac
    31741 tccaacagca ttgtttggga tgattgttcc aactatagga atacttgttg taggctctgt
    31801 atttataata ttatttactg caattgtagg aaaactattg ggatattctc caagaatgtc
    31861 agctgcaatt ggtgtaactt gtatgctagc ttatcctgca acacagataa tttccaacga
    31921 aggtattgat gctatggaag gaactgaaga agaaaaacaa agggcaacag attatttatt
    31981 accaaagatg attataggag gatttgttac tgtaaccatt gcatcagtgg catttgctag
    32041 tataataggt ccaattatat ttggttgata gtaaatatac ttataggagg aataggaaat
    32101 gataggaagt atatctataa atgagataga taaaaaaaga ttcaaacttg atgatttaag
    32161 taagaaaata tgggaaaatc cagaaaaagc atttaaagaa tttaaagcat gtgaaaatac
    32221 agcaaacttt ctaagaagtg aaggatttga tgtggaagta ggtgtaggag gtttagccac
    32281 ggctattaga gctagttttg gttcaggtaa accagttata ggttttatgg gagaatttga
    32341 tgcattacct ggtttaaatc aaaaagtttc aacaaagcaa gaagcatttg aattgggtgc
    32401 atatggtcat ggatgtgggc ataatttatt atgtacagca catgtagggg cagtagtagg
    32461 gcttaaaaga gagatgatag aaaataactt aagtgggact atagtatttt atgcatgtcc
    32521 aggagaagaa caattaacag gtaaaggatt tatggctaga ggtggagcgt ttgaagggtt
    32581 agacttagct ataaattttc atccaaataa aattagtgaa gctacaattg gtacttcaac
    32641 agctgttaac tctgtaaagt ttcactttaa gggaaagact gcgcatgcag gctcagaccc
    32701 tcaaaatgga agaagtgcac tagatgctgt ggaacttact aatgtgggag ctaattattt
    32761 gagagaacat gttacttctg atgtaaggat tcattataca attacagatg gaggagtagc
    32821 acccaacata gttcctgata aagcatcagt atggtattat acaagagctt taagtagaga
    32881 agctgtagaa agtacatatg aaagacttgt aaaggtagca aaaggagctg caatgatgac
    32941 tgaaactgaa gttgaagtag agtttttagg aggttgttat aatactttaa acaatcatgt
    33001 tttagcaaat cttgtatcag aatgtatgaa tgagattaaa caagagcctt ggactcgaga
    33061 ggaattggat tttgcagctg aattagatag acaaagccca caacaatata aggctatgat
    33121 aagtaaatat aatttgccag aaggaactca tctatattat ggtggaggaa atgttacaaa
    33181 ctttaatagt tatggttcta ctgatattgg agatgtaatg catatagttc cgacagcata
    33241 cttttttaca gggtgtacga atctaggagc accaggtcat agctggcagt ttacatcttg
    33301 tgcaggaagt tctataggag aaaaaggtat gatatatgca tcaaagatta tggctatgtt
    33361 tggagctaaa atattaaata atccagagat agctaaaaag gcaaaagaag aatttgataa
    33421 atctatgaat ggtaaaacat ataaatgtcc tattccagat gatattccaa ctccttaaaa
    33481 agacatgaaa tttaatatat aaaattataa tttttatatc agcatcattt cattactaaa
    33541 agaaagtgag attttgctga ctatctgatt aggaggaagg aatatgaaaa atttttttaa
    33601 agaagcgcag tcaatagaag gggaaattat tagatggaga agatatttac atgaaaatcc
    33661 cgaaattggt tgcaatatac cgaaaacagt aacctttgtt accagtactt tagaaaaaat
    33721 gggatattct ccgaaattga cgtctaataa tggaatagta gctgttttag acagtgaaaa
    33781 tggtggaaag accttattat taagggcaga tatggatgca cttcctatta tggaggagac
    33841 ggatttacct tttaaaagta agaataaaga tgcatctcat agttgtggac acgataatca
    33901 cacagcaatg ctcttaggag cagctaaaat acttatggat aataaacact tacttaaagg
    33961 tagagtaaag tttgtatttg aaccagatga agagactacc agaggtgcaa aatcaatgat
    34021 tgatgatggt atattagaaa atcctcaggt cgatgcagct atggcatttc acagtatggt
    34081 gggtaaattt cttaacacag gtcaagtagc ttattcacgt ggtccagcta tggcatctgc
    34141 ggatatattt gaaataacag tagaaggaaa agcaactcat ggagcaagcc ctgaatttgg
    34201 agtggaccca ataaacataa tgtcgcatat acaaatagca ttaaacacta tactttcaag
    34261 agaaaaacct cagaaagaac ctgtagtttt aactattggt atgtttaatg caggaaacgt
    34321 accaaatgct ataccaaata aagcatctat gtcaggaact ttaagaacat ttgaccaatt
    34381 tgttagagaa aaattaaaaa atagaattat tgaaatatct acaggaatag ctaaatcatt
    34441 tggaggaaat gctaaagtgg aatttaaaat ggggactcct gcagttataa atgatttgga
    34501 tgtaggagat gagattgtat cttatgttaa agaagttata ggaaatgaaa atgtaataga
    34561 ctttccatct gcaatgggtt cagaagattt ttcagagatt ttactaaggg ttccaggagt
    34621 atttttatgg attggaatgg gaagtattag tcagggatat aattttggta tgcataatcc
    34681 taaagcaatt tttaatgaag agggattagt ttatggttca gctttatttg ctcattgtgc
    34741 agtaaaatgg ttagaaaata attttagtag ataataacat aaaatattat taaatatcat
    34801 aaaaggtata tcttaaaaag atgcaaagat atacctttta gaaagtcata tataatttta
    34861 cttaatatta gatgataaaa tatttttatg cattttggat tttaatattt attttctgat
    34921 ataaataatt tgtataccct ttcaggtcta ccttttgttg agttttgttt ttcatataat
    34981 atagtagcgt ttccagactt taagagacta cttagaaatc tattagcagt tctaactgtt
    35041 acatttaaac atgaagataa ttccttagga gtaacttcat ctctttgtag aatttcagta
    35101 acagatataa tcttttgaat agtaagagta gacaatttag aacttttggc aattgtattt
    35161 atatgtggag ttactttatt tgaaacagta agtaacttat cacacagtaa aggtcctatt
    35221 gtctcataat tttcgttaac taagaaactg taatttaaat gatttatttt agattcctta
    35281 ttagcactta tggcactaga catagcttgc tttatatcat taccaattcc atatccaaca
    35341 catactttaa tatttgtact attttcaata gattctttta tataacatgc agtaaaattt
    35401 ttagttaatg atttaatcgt ttcacattta gtgaaaattt taaaagaatt actatttttt
    35461 tcaattataa aatcggttaa tttatctttt ttaatactat ttaatatttt ataaacttgg
    35521 tctagagtag agtcatcata atttgtatct ttaactgtta tatatattac acttggttga
    35581 tttcccttca ttctatgtaa atgaatatct tttattaagt tattaaaagt attttttaat
    35641 aaatctatat cagggtatat gaaatgaaag tttactcctg cattttgtaa cataggaaca
    35701 atactactaa aacgagtagt agaaaaatcg attttccctt cattccatag attaatatgt
    35761 ttttttgcaa tctttttttc aagttttgct atgtcagata aactcatttt agatgcattt
    35821 tcatcaatac tatccattaa gtctccaaat gtgccatttg ataaataata tgatatatct
    35881 tttggattat ctgcccagtc tgttaagtca aagtacgctc tagaaaaatc taatgtatta
    35941 tatttaaaga taagctttag aaacatatca tagtaacttt gtaagtcagt accaaaatca
    36001 actagaggtt ttttaatagt tccagccttt tttgtaatag cttttttggg gatagggcca
    36061 ctaactgcaa atccatcaaa cctatcctct atttccaaat ataaatctcc aacatgagaa
    36121 aaatctctat agatatatat ttcaatttct gcattaatat ttaacttttt tatagaatta
    36181 tctacaaact cttttaaaaa ttcggttgtt atgactgcta ttttcatcat agattacttc
    36241 cttttcaatt attataatta ttttatatta aatattacat taatctattg ttctagttaa
    36301 aatcgtattt caattaaatt caattaaaat aaataactct ttataaaaag caattattta
    36361 ggtagaggaa tatttgctaa ctttaatagt ctttgttaac tttaatttta tttaagttat
    36421 tatagtctta ctaattttat aattgtagat ttaactactt tatgttcttt tattgtttat
    36481 gtagttgatt tagaagttat tttaaccatt tattttaatt gaagttgttt gacttttaaa
    36541 tcctttgtgt aatctttgtt tttctagcca tgataaaagt atttctattt gtatcactat
    36601 atataataat atcatgacaa aatatttatt gtctaactaa ttataatcta tccaaaatga
    36661 aaatgggtgt gagatattat tacaacaaat tgtattagta ttaaaaagat taaaaaatta
    36721 aaaaactaaa cagttaaata attaaacgat taagtgactg tcatataaca atttataatt
    36781 ttatttaaca gccacttagg tatttaagag cgatagaatt gagtgtatat caaattataa
    36841 taatttccta gtaaataatt gaaaaataag gtagtccatt tttatatttg atatctggtt
    36901 taccttgaat aagtggatat agataattta gggcttcatt agttatataa ttacattctt
    36961 catttatcca ctctttagga aaatattttg tcttgttagc tattttagaa atatcagtat
    37021 gactaacttc atacttataa ggattattgc atgttctatt aatgatagcc ataactcctg
    37081 tatatccttt tttagatagt tctatcccat atgcaccaac ttcagctgct tgttttaaat
    37141 cagtatctga agctaaatgc atagcacatc tttgtattac accaagttct aaggttttta
    37201 ctcttgttgt aattgatgat gatactataa gattctttag atagttgctt actccaccta
    37261 attgggaatg accaaattta tcatgagcag aagttttttg ttcgcatata aagttgccgt
    37321 atttatccct tataccttct gatgcaacta tataagcctt atttttttct ttaaatattt
    37381 ttctaacatc ttctataaac ttatcttcat agaaatcaat ttctggaagg tatattaaat
    37441 caacaagtgg agtatcatta agtctagcta gtgaagaaga agctgctaac catccagtat
    37501 ctctacccat agtttctact ataaatatac cattattttt gtatatagaa aaatctaagt
    37561 atgtttccat tattagtgta gaaataagct ttgcagcaga accaaatcca ggacagttgt
    37621 cagttcctaa taaatcatta tctattgttt taggaatacc tataaatttt attttacaag
    37681 tagtgttttt gcagtattga gaaaatttca tcactgtgtc catagaatca tttccgccta
    37741 tataaaataa tgtatctatt ttatattcat ctagtatatt tataactcta tcataatcat
    37801 aagaatttat tttataatca gccagtttgt atctacaaga accaagacca gacgaaggtg
    37861 tttgctttaa atttaataca ttatctttgt ttaaggagtc tgaatctatt atatttttgt
    37921 ttaatatacc ttcaatccca ttgataccaa aatatacttt atcaaagtat ttagtttcta
    37981 aatttttttc ataaatacca actgcagaag cattgataac agctgtaggg cctccagatt
    38041 gggcaataat acagtttctc attttacatc tccctttcat taatattgtt atgaatggta
    38101 ctttttatcg agtaaattta tatttatagt ctgattatat attaaatgaa ctagctatat
    38161 caattgagtt agtatagaca ataaatgcca taagaatgtg gtaaaaaact aactttttat
    38221 atacaattaa tgtctataat ttaccacaat ataaaggtac ttttaaggag tagtcaaagt
    38281 gttgtgaaaa aaaatatatc agtgtaatat taatttacaa agcaaaataa gttcacagtt
    38341 aacaaaagca gaataagttc ataaaagaaa tatatacagc aataaaagta atgctaaata
    38401 aaagtaatac tggattaaaa aatattaaaa aaatatcaaa aactaatatt taatactaat
    38461 taaagtaaaa tatggaggta tacaaatatg aaacacataa aaatagcagc aggattagca
    38521 catgtaaatt acggtcatat atcagccgta gttaaagaag caatggatgc aggtgtagac
    38581 tacgtacatt cagatgcagc agatatgcat gacttaaaaa atatgcaact tatgggtggt
    38641 catcaaataa tagaagcaat aagaccagtg acagataaac caatagaatg ccatatatac
    38701 acaagagatt gtgatagatt atttatagaa aaaatagcag cagcaggatg taacttgttg
    38761 ataataccag cagaacattt tttaggtgct cctcttgcat acataataaa ttactgtaga
    38821 gaatttggta tgaaagtagg attaacaatt ggatgttaca caccattatg ttttgttgat
    38881 gaagctatat atgatataga cagattgcaa atagtcgttc atggtgttga tgaaactgat
    38941 ggaaaagata actggggatg gagaaagagt gctgttgatt tagtaaaaag agctagaaaa
    39001 atgatagacg aaaagaatcc taagtgtgaa ttagcaatag atggtggact aagatcagac
    39061 aatatggagc cattaataga atgtaaccca gacgttgtaa tattatcttc agcaatattc
    39121 aaggataaag atggtataac agcaggtgtt aagaattgta gaaaagctat agatgaagca
    39181 gctacaaagt ttggtttaga ataagataat aaactatgat aaaagagatg ctttcttaat
    39241 gttttaagga agcattaatt ttatgaaaaa ctacaggaag ctagactaat ttaattgatt
    39301 ttttatactg aaacaatata atcataggag gttattaaat gaaaaaagaa atgataaaag
    39361 attatttact gataacaata tcagcatttt taatggcatt agcagttaat tttttctttg
    39421 cagaacatac attagctcca ggaggtataa caggattatc agtcgtactt agcacattct
    39481 tatcaatgcc tgttgagaat atatcattag gaatatctat gccactttta gtaatgggat
    39541 tattattttt agggaaaggt tttggaataa aaacattata tataactctt atgggcccat
    39601 tattcttaaa agtaatacca caaactcaca taactgacaa tctattagta gcaggagtga
    39661 taggtgggtt attggtagga actgctatag ggatagcaat aataaggggc tgtagtactg
    39721 gtggaactga tttagttgca atgcttatta ataaggtaat taagtttctg aagttgccag
    39781 ttatattatt tatgctagat ggatttataa tagtagcgtc tggaataata agtaagaatt
    39841 ttatgatatc tatatattca ttgatatctt tattactcat aataaaaaca ataggatttg
    39901 taactacaaa gtttggtaag aatactgaag aaattaataa tataaataat gcttgtgctc
    39961 aataaaaata ataaaattta ctttaaatat aaaagtttaa aaaatataaa agcttaaata
    40021 aaaagcttaa ataaaaagat taaatataaa aaatggaggt gtctcaaaat gaatttttta
    40081 gttcatgagg cactttttct atgaaatcaa atatattttt atcatttaga caatgaaaaa
    40141 taggtttatc tctatgttga gacaagccta tttttgttta tttataagat aatattttat
    40201 ttttttcaga atatagtttt tttataattg tactaagtct agaaatatca ttcttactta
    40261 aaagagcatt tatatttatc agttcagcat tatgttcaaa gtctacagga gcagtggtta
    40321 gtattaagtc cacatcatca aagttcgtaa atgaaataga agatagaggt atagatttaa
    40381 ctatattaat ctgattaaat tcaaaactta acttttggac taataaatta gatacaccat
    40441 cactacagtg acatattaaa atagtattaa ggggagattt gcttctatct attgctgata
    40501 ttaaatataa agcaagatat cctatttcgt catcattgac ttcataattc atcaactgtt
    40561 tgataacagt attagactct ttagcagcct tataagtata aggaaatgtt ctctttatat
    40621 catttaatat gggatttgta atagtaaacc catagtttat tctgtgtaga actgatttaa
    40681 ggtgattagc aagagacttt attaatatta aatcatcctt taagtttaaa tttagtatac
    40741 ttccccaatg aacagctatt gcttcagata gattatactc tgtaatttct tcatttttaa
    40801 gaggttcaag agtagtagtt ttctttattg atttaagtaa tccttctata tgaagaagga
    40861 tataacagac ctcttcatca ataaattcta cgtcaaaagc ttcacttagg ttagcacata
    40921 aaatttttga tttttcaaag tattcatgtt ctaataattc tctaaaaaaa ccttcagata
    40981 agttaatata tcttttaatt gaaattcttt taatacatat agctatatgt attaagaagt
    41041 ttatataata ctcatcacta aggttacttt tttctacttg taaggtgtgt agagtgattt
    41101 gagataattt ctcaaaatca tagttaaata gctccttaaa tatttttgtt gatggggtat
    41161 ctttgcattc attagatttc gagaataata tttctttaag ttcagtataa cttttattaa
    41221 ccgtagcaag ttcaaaaatt gcttttcgaa tatctttctc ttttccctct aaatatactc
    41281 cattattggt ttttctaact agagtcactt taaagttttg caaaaaattt tgaataacta
    41341 ccaagtcttt atgcacagta gctctactaa catgtagttc attgcaaata tcacgaatcc
    41401 taaacttaga atttttaata aataatttac ttagaatata cctttttcta tcttcaggag
    41461 agtatgcata tatacaattt gatttatcat tgatatctct aataacttta agttttatat
    41521 cttcattacc tagaataata acccctgaac cagttttttt atctagggat aaattaaact
    41581 ctaaaagcca ttcatctaaa ataacaaggt cattacgaat agttttatta ctaactttta
    41641 attcttcggc aatagtatct acagtaatat agtctgagct gttgagtaat aattcagcaa
    41701 tatctaataa tcttttgttc aatgcaaacc ctcctaaatc ttagttacta tttattaacc
    41761 ttggaccaat ctctttcaaa agtattggca ccatctattg taagctggtt aaaagaagct
    41821 tttttaaata aatcatatgt tacagctgca taatcacatc cacaaagaga tgcttgagtt
    41881 aagtggaata catttttaat agaagctgca acaactttag tattatatcc atttattgat
    41941 ataaggttac atatttctgt aaggtttttt ataccatcat tcccattttc atcatttcta
    42001 cctataaaag ggaataggta atcagcacct gcatttatag ctaatgatgc ttgatttaca
    42061 ttgaaaatca aggtacatgc agtttttaca cctttactat ttaatatgtt tataagttct
    42121 aagccaactt cagagaaatt tatttttatt atagtgtttt tatttatatt taatatctta
    42181 tcacatgaat taattaattc gtataaatta tttccaatga cttcagcagt taatatttct
    42241 ggatttaaat tagcacatgt ctcaataaat tcatggaagt ttactttctc tttaagatac
    42301 atagatggat tagcggttat tcccttaaat ccatacttta gacacttttg aatttcatca
    42361 agattagcac tgtctatcaa atatttcata atttaattct cctttaagct atttgtcata
    42421 aaaaagtgta tatactctat ttttattatt aagtcaatta tacaataaga ttgattataa
    42481 aattataaca tgggtactta ataaaattta cataatgata tggtaataat aaaacattga
    42541 ttgtctaata attaccataa gctttagtaa ataagagtta acagtgaagg tattgcctta
    42601 aaaatcctat gatatgtata atttgaatat gaaggcaata atttaaggga ggaaaaatat
    42661 gattaacttt tatcaaccat caagaataaa ttttggacaa ggaactttaa gtgaattacc
    42721 tagaataata ggaaaatatg gaaagaaatg tttgttagtt actactccaa atgtagagcc
    42781 attagacaag ttatataaaa gagttacatt gaatttaata gaaaaaggta tagaagttgt
    42841 acactttgat taaaaaatag ctacgcacac tcgtgacttt agtcatgagt taatagctaa
    42901 aatagtcagc atatagggaa acctatatgt agagatagga tagaacctat ccaatactac
    42961 ttgaattgct ggaaacccct aaagctaacc aaactaaaac ataaggatga aataaaccta
    43021 agtgtgaaag ttacgaaagt agaaaaaatt ggttagatgg tataaggtta aatcctaagt
    43081 actttaaaat gggcaatcag caggtaagct ccgaatagga gaaacttcaa cgactattcc
    43141 tcttgaggga agtacactac aagttatcgg tagtggaagt gggtagacct taacggataa
    43201 tgccgaagga taagatatag tctgtgcttg catgaaagtg taagaagttc ataagagaac
    43261 tgcataggta gtagcgaact tatgtgaacg acaacctcaa aaatgataaa agttaagact
    43321 tacggttctt aaatatgtta tttaaaatta aatataaaac cttctaaata attgttttaa
    43381 ataattacat ttgttaatat tatgataggt gattaataaa ttgttcacca cagcattata
    43441 taatatatta aatattaaaa tcattaaaaa gtgtatcaaa aaacaattaa agtgaggtga
    43501 tagcagtgaa aaaagcttat aagtttagaa tgtatccaaa taaaaaacaa caagagttaa
    43561 ttaacaaaac ttttggatgt tgtagatttg tatataataa gtatcttgct aaaagaatag
    43621 aagtttataa aaacgataaa gaaactttta cttataaaca gtgtagttct gatttaacca
    43681 atttaaa
//
LOCUS       DAGSWI010000017        43081 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.017, whole genome shotgun sequence.
ACCESSION   DAGSWI010000017 DAGSWI010000000
VERSION     DAGSWI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43081
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.017"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            170..1363
                     /gene="nifV"
                     /locus_tag="MJF80_000847"
     CDS             170..1363
                     /gene="nifV"
                     /locus_tag="MJF80_000847"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBZ0279520.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1388..3313
                     /locus_tag="MJF80_000848"
     CDS             1388..3313
                     /locus_tag="MJF80_000848"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBZ0279521.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3335..4330
                     /locus_tag="MJF80_000849"
     CDS             3335..4330
                     /locus_tag="MJF80_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBZ0279522.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4656..5129
                     /locus_tag="MJF80_000850"
     CDS             4656..5129
                     /locus_tag="MJF80_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBZ0279523.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5163..5657
                     /locus_tag="MJF80_000851"
     CDS             5163..5657
                     /locus_tag="MJF80_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0279524.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5754..6275
                     /locus_tag="MJF80_000852"
     CDS             5754..6275
                     /locus_tag="MJF80_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBZ0279525.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYSD
                     SDLDYKKVHFNNF"
     gene            6464..6775
                     /locus_tag="MJF80_000853"
     CDS             6464..6775
                     /locus_tag="MJF80_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBZ0279526.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6803..7489
                     /locus_tag="MJF80_000854"
     CDS             6803..7489
                     /locus_tag="MJF80_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBZ0279527.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
     gene            7721..8605
                     /locus_tag="MJF80_000855"
     CDS             7721..8605
                     /locus_tag="MJF80_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBZ0279528.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            8704..9879
                     /locus_tag="MJF80_000856"
     CDS             8704..9879
                     /locus_tag="MJF80_000856"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBZ0279529.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            10007..10861
                     /locus_tag="MJF80_000857"
     CDS             10007..10861
                     /locus_tag="MJF80_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279530.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            10905..12140
                     /locus_tag="MJF80_000858"
     CDS             10905..12140
                     /locus_tag="MJF80_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBZ0279531.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            12241..13182
                     /locus_tag="MJF80_000859"
     CDS             12241..13182
                     /locus_tag="MJF80_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBZ0279532.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(13401..13754)
                     /locus_tag="MJF80_000860"
     CDS             complement(13401..13754)
                     /locus_tag="MJF80_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBZ0279533.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            14050..14961
                     /locus_tag="MJF80_000861"
     CDS             14050..14961
                     /locus_tag="MJF80_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBZ0279534.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            15221..15655
                     /locus_tag="MJF80_000862"
     CDS             15221..15655
                     /locus_tag="MJF80_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279535.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            15678..17213
                     /locus_tag="MJF80_000863"
     CDS             15678..17213
                     /locus_tag="MJF80_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279536.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EVSLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTLKSMVTVSKNKNEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            17297..18664
                     /locus_tag="MJF80_000864"
     CDS             17297..18664
                     /locus_tag="MJF80_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0279537.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFIGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            18699..18923
                     /locus_tag="MJF80_000865"
     CDS             18699..18923
                     /locus_tag="MJF80_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBZ0279538.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(19371..19769)
                     /locus_tag="MJF80_000866"
     CDS             complement(19371..19769)
                     /locus_tag="MJF80_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBZ0279539.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(19772..20173)
                     /locus_tag="MJF80_000867"
     CDS             complement(19772..20173)
                     /locus_tag="MJF80_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBZ0279540.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            20533..21519
                     /locus_tag="MJF80_000868"
     CDS             20533..21519
                     /locus_tag="MJF80_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0279541.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            21503..22414
                     /locus_tag="MJF80_000869"
     CDS             21503..22414
                     /locus_tag="MJF80_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0279542.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            22527..24095
                     /locus_tag="MJF80_000870"
     CDS             22527..24095
                     /locus_tag="MJF80_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBZ0279543.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            24170..25195
                     /locus_tag="MJF80_000871"
     CDS             24170..25195
                     /locus_tag="MJF80_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279544.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKAEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            25188..26132
                     /locus_tag="MJF80_000872"
     CDS             25188..26132
                     /locus_tag="MJF80_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279545.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            26491..29148
                     /locus_tag="MJF80_000873"
     CDS             26491..29148
                     /locus_tag="MJF80_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0279546.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            29363..29992
                     /locus_tag="MJF80_000874"
     CDS             29363..29992
                     /locus_tag="MJF80_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279547.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            30127..30453
                     /locus_tag="MJF80_000875"
     CDS             30127..30453
                     /locus_tag="MJF80_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0279548.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            30469..31758
                     /locus_tag="MJF80_000876"
     CDS             30469..31758
                     /locus_tag="MJF80_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBZ0279549.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            31782..32111
                     /locus_tag="MJF80_000877"
     CDS             31782..32111
                     /locus_tag="MJF80_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBZ0279550.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            32259..33587
                     /locus_tag="MJF80_000878"
     CDS             32259..33587
                     /locus_tag="MJF80_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBZ0279551.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            33904..34764
                     /locus_tag="MJF80_000879"
     CDS             33904..34764
                     /locus_tag="MJF80_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBZ0279552.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            34969..35949
                     /locus_tag="MJF80_000880"
     CDS             34969..35949
                     /locus_tag="MJF80_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBZ0279553.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            36152..36601
                     /locus_tag="MJF80_000881"
     CDS             36152..36601
                     /locus_tag="MJF80_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279554.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPHIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(36762..37691)
                     /locus_tag="MJF80_000882"
     CDS             complement(36762..37691)
                     /locus_tag="MJF80_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0279555.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            37884..38696
                     /gene="modA"
                     /locus_tag="MJF80_000883"
     CDS             37884..38696
                     /gene="modA"
                     /locus_tag="MJF80_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBZ0279556.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDNGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            38845..39528
                     /gene="modB"
                     /locus_tag="MJF80_000884"
     CDS             38845..39528
                     /gene="modB"
                     /locus_tag="MJF80_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBZ0279557.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            39529..40605
                     /locus_tag="MJF80_000885"
     CDS             39529..40605
                     /locus_tag="MJF80_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBZ0279558.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            40611..41168
                     /locus_tag="MJF80_000886"
     CDS             40611..41168
                     /locus_tag="MJF80_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBZ0279559.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(41332..42354)
                     /locus_tag="MJF80_000887"
     CDS             complement(41332..42354)
                     /locus_tag="MJF80_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBZ0279560.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(42485..>43081)
                     /locus_tag="MJF80_000888"
     CDS             complement(42485..>43081)
                     /locus_tag="MJF80_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0279561.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
BASE COUNT        16974 a         4533 c         7707 g        13867 t
ORIGIN      
        1 atgatacaaa gtaatataat agattacaag atatgaaaag ctaaaaaaat aaattgcaaa
       61 tgaaattaaa atgaataaaa aataaaaaaa taatcataag ttagaggtat caatgaaaaa
      121 taagacttaa tgtttttaaa attaaaagta tactaagggg ggcattaata tgtgcgttat
      181 atcaaaagat agagcaaagg aaataaaaat agtagatact actttaagag atggagagca
      241 aacagctggg gttgtatttg caaatagaga aaaaataatg attgctgaaa tgttaagtga
      301 cctaggtgtt gaccaaattg aagttggtat acctactatg ggtggagatg aaaaaaatgt
      361 aataaagcac atatgtagta gaaatttaaa atcagatata atggcttgga atagagcagt
      421 aataaaagat gttgaagaat ctatttcttg tggtgttgat gcagtagcta tatcaatatc
      481 agtatctgac attcatatag aaaataaatt gagaacttct agaggctggg ttttagaaaa
      541 tatggctaaa acagttgaat ttgcaaagaa aaatggatta tatgtttcag taaatggaga
      601 agatgcttca agagcagata tagacttctt aactgaattt ataaatgttg gaaaacaagc
      661 aggggcagat agatttagat actgtgatac agtaggagtt atgaatcctt tttctataaa
      721 gaatgcaata gaaactcttt atgaaagaac taattttgat atagaaatgc atacacataa
      781 tgactttggg atggcaactg caaatgcact tgcaggaata gcagcaggag caaattatgt
      841 tggtgttaca gtaaatggat taggtgaaag agcaggaaat gcagcattag aagaagtttt
      901 aatggcactt aagtgtgttt ataaatgtga tttaaataat atagatacta gaaaatttag
      961 aggaatatgt gagtatgtag cacaggcatc aggaagaata cttcctacat ggaaaccagt
     1021 agttggggat aatatgttta tacatgaatc tggtatacat gcagatggag ctttaaaaga
     1081 cccacataac tatgaaccat ttgacccaag tgaagtaaat cttgaaagaa aaatagtaat
     1141 aggtaaacat tcaggaagag ctgcagttgt aaataagttg agtgaatatg aaatgtatat
     1201 ttctccagaa aacgctacca agctattaaa tgctataaga gctacatcta taagattaaa
     1261 aagaagttta atggataaag aaattcttca attatattgt gatatactag cacatgagaa
     1321 aggcacaaca gaagaagaag cagttagagg ttcatatatt taagatataa ttgtttagga
     1381 ggaaaatatg ggagataata tagtttataa aataattaaa aaacatatag tagatggaga
     1441 agctgttgct ggaagttcaa ttggtataaa aatagaccaa actttaacac aagattctac
     1501 tggaacaatg acatatcttc aactagaagc tatgggaata gataaagtaa agactaaaag
     1561 aagtgtggca tttgtagacc ataatatgtt acaacaagga tttgaaaatg cagacgacca
     1621 taagtatata caaacagtgg cagataaata tggagtttat ttctctaagc caggaaatgg
     1681 tatttgtcat caagtatttt tagaaagatt ttcaacacca ggagatacac ttttaggttc
     1741 tgatagccac acacctactg ctggaggtgt gggcatgatg gctattggag ctggtggatt
     1801 agatgttgct ttagccatgg caggaggagc atactatata aaagcaccaa aagtctgtaa
     1861 ggtgaatcta gttggaaaat taaataatat ggtatcatca aaagatataa tattagaagt
     1921 gttaagaaaa caaactgtaa aaggtggagt aggtaaagtt tatgaatatg gtggagaagg
     1981 tgtcaaaagc ctatctgttc cacaaagagc tacaataaca aatatggggg ctgaacttgg
     2041 agcaactact tcaatcttcc caagtgatga aaaaacacta gaatttttta agagtcaagg
     2101 aagagaagac gcatgggtag agctaaaacc agatgcagat gcagtttatg atgaagagat
     2161 tactataaac ttggatgaat taaaaccatt agctgcaaaa cctcatagcc cagacaatgt
     2221 agatgaagtt gaaaatatag gcaagattaa aatagaccaa gtggcaatag gaagttgtac
     2281 aaattcttct tatgaagact taatgaaagt agcacaaatc ctgaaaggaa ataaagtaca
     2341 taaggatgtg agtttggtaa tagcaccagg ttcaagacaa gttatggaga tgattgctag
     2401 aaatggggct ttggcagata taataagtgc tggagctaga atactagaaa attcttgtgg
     2461 tccatgtatt ggtatgggtc aatctccagg tactgactct gtgtctttaa gaactttcaa
     2521 tagaaatttc tatggtagaa gtgggacttt gtcagcacaa gtatatttgg taagtccaga
     2581 agtggcagca gtaagtgcaa tcaagggagt gcttacagac ccaagagaat ttgatattaa
     2641 atttacaaat ttagatgtaa atgagttttt gatagatgat agcatgataa ttaaacctgc
     2701 tgatgtagga agtgatgtag aagtagttag aggacctaat ataaaacctt tccctcttaa
     2761 tacagagcta agtcagtcta taggtggaaa agttatatta aagacagaag ataatataac
     2821 tactgaccat ataatgccat caaatgctaa gttacttcct tttagaagta atataccgta
     2881 tcttgcaaac tattgtttca atactgtaga tacagaattt cctcagagag caaaagacaa
     2941 taatggtgga tttatagttg gtggagataa ttatggtcaa ggttcaagta gagaacatgc
     3001 agctttagca ccattatatc taggtgttaa aggggtaata gttaagagct ttgcaagaat
     3061 ccacaaggct aacttgataa atagtggtat aattccaatg gagttttgtg atgagaaaga
     3121 ttatgaaaat atatctcttt tagataatct ggagatacca aatattttag acaatctagg
     3181 tagtggaatt ttagaagtta agaatacaac taaaggaact tcttttaagg tcaaagtaga
     3241 attgtcagca aaagaagttg atgtattaaa agctggtgga aaattaaact acactaaaaa
     3301 tcaagctaat taatatgaaa agagggaata ttgaatgtat aaagtgacac ttataccagg
     3361 agatggaata ggaccagaag ttgcgaaagc tatgaaaaag gtagtagaag ctactggagt
     3421 agaaatagaa tgggaagaag ttaatgcagg agaggcagtt attgaagagt atggtactcc
     3481 acttcctgaa tatattatag atagtataaa aaagaacaaa atagctataa aaggcccaat
     3541 tactactcct gtaggtaaag gatttaggag tgtaaatgta actttaagac aggcattaga
     3601 tttgtatgtc aatttaagac ctattaaaag ttttaagggg ataaaatcaa gatatgaaga
     3661 tgttgattta gtggttgtaa gagaaaatac agaagattta tatgctggaa tagaacataa
     3721 aataggtgat tatgcagctg aaagtataaa gataataaca agaagtgcaa gtgaaagaat
     3781 cgttgatttt gcatgtaact atgttaaaga taataagaga aaaaaagtta cagctataca
     3841 taaggcaaat ataatgaaaa tgtctgatgg attgttttta gatgtgttta gagaagttgc
     3901 atcaaaacat ggtgtagaat atgatgattt gattgtagat gcagcagcta tgaacttagt
     3961 tttaaatcca gaaaattatg atgtaatggt aatgccaaat ctttatggag atatactatc
     4021 agatttaggt gctgggcttg ttggaggttt aggtataatt ccaagtgcaa atattggtaa
     4081 agattgtgcg atatttgaag ctgttcatgg aagtgctcca caaatagcag gtcaaaataa
     4141 ggctaatcca acagcactta ttcaatcatc agtaatgatg cttagatact taggtgaata
     4201 tgaaaatgca caaaaaatag aaacagcttt agaaaaagta tttcttgaag gaagtaaatt
     4261 gactgtagat ttgggtggct cagcttctac tacagagttt gcggatgagg tgtgtaagta
     4321 tattgtatag attttattta gggaaaaagt aaaatataga ttgaaggaaa gaggctttct
     4381 caaaatagag ataagccttt tttattggaa tgataaaata tatttgattt tatacaaaag
     4441 agagttcctc ataaacgaaa aagttaattt tgggatatcc attttaattt taaaattaat
     4501 tgattaatat gcaaagaaag attaaataaa tttattattt ttaattgaac tttcaaccaa
     4561 aatatggtaa aatttaaaaa agttgaactt tcaactaaaa atgaatatat tgattatgtg
     4621 aaattctata aaacagaaac tatttgactt tagccatagt agtttcagtg gaggtgtgtt
     4681 tgattaagac tttagacagt aatatattaa gagaagtagg tactttatct agagcagtaa
     4741 attcaataaa tgatattaag tacaaggaat taaagttaca aaaaggacaa tttacatttt
     4801 tgacaagaat ctgtgaaaat cctggtataa atcttgtgga attatcaaat atgttaaagg
     4861 tagacaaggc tacaacaacc aaggctatac aaaaattaat aaaggcagga tatgtagata
     4921 aaaagcagga tgagtttgat aaaagaggat ataatttaac tccaactgat aaatctttgg
     4981 aagtttatga gttgataatt gaagaagaga atagaagtat agagatatgt tttgataatt
     5041 ttacagatga agaaaaacaa gtggtaacta agttattaga aaaaatgagc aaaaacgtag
     5101 aaaatgaatg gtttaaagta aagagatagt aaataaatac attaacttaa ggagatattt
     5161 ttatgattag aaaagcgaat atgaatgatt tagaaagcat tatgaaaatt ataaaatcta
     5221 cagtggaaga aatgaagact tataataata cacaatggga tgaaaactat ccattagaaa
     5281 aagactttgt ttcagatatt aagaaacaag atttatatat ttatgaagtg gatggagaag
     5341 tagcagggtt tatatgtctt aattatgaag agccagaaga atatttaggc ttaaattggt
     5401 catctaataa aaaagcaatg gtaatacata gaatggctgt aaatccaaac tttagaaaga
     5461 caggaatagc atctaagtta gttgattttg ctgaaaaatt agctgtagaa aacaatgtat
     5521 catacttaaa gagcgataca tattcaataa actcaaaaat gaatttatta cttacaaagt
     5581 gtggttttat aaaaatcgga gaaatgagtt ttttaggaaa ggagaaatct ttctactgtt
     5641 atgataaaat attataaaga gcatttacaa tacaaggggt aaataataga ttttcagcat
     5701 tagtaagggc ataatataac ctagtatatg ctattatgaa agggatgatt tatatgattg
     5761 gatttctaaa gaaaagaaga agtattagaa aatacaagga tgttgaagta gaaaaagaaa
     5821 aattagataa aatattaaaa gctgctcttt tagctccatc atcaaagggg cttagaacat
     5881 gggaatttat agtggtagat gataaagaaa agctaattaa tttatctcaa tgtagaacta
     5941 aaggtggtgg atttttctta aaaaatgcac ctttagctat tgtaattatt gcagataaag
     6001 aaaaaaatga tgtttggata gaggatgcaa gtatagctgc ttcatacata caacttcaag
     6061 ctcatgaact aggtttaggt tcttgttgga tacaagtaag aaatagaatg catgatgata
     6121 atatagaagc tgataagtac attagagaag aattaaaagt acctagcaag tacagtgtag
     6181 aatgtataat ttctatagga tatcctgatg aagaaaagaa agcatatagt gactctgatt
     6241 tagactataa aaaggttcat tttaacaact tttagtatat aatatgttgt attaagacat
     6301 tatatttttt tctattgtta atatatactt aaaattgtaa aaatatatct gaattttact
     6361 aacttatcta ggggttatgt acaaggattt taaagataaa tatagaaatt aatgtttata
     6421 atcgatacat aacttgagta acaaaattta aggaggttaa agtatgggta tatttaaaac
     6481 aaaaatggat gaggactgga aagtaaacta cattaaagag tttaatgaaa tgagagattc
     6541 ttatgagagt aaacttcaaa aaaaacagtt tgaggtagat agtttgaaat cagaattgga
     6601 tagactaaga agctataaaa atagccttaa gccaaaagaa aaacaaatta ctgatgatga
     6661 tataaataat ataaagagtt taagaagaga tggcttaagc tataaagaaa tttcaaatca
     6721 aacaagttgg agcaaagcaa ctgtaagtag ggtattaaat ggactttacg attagcatat
     6781 acatggggtg atttaaattg ggatgaagta tagttacaaa aaatgcataa tagattctat
     6841 tttattgatg tttatagttc aaatattaag gatgattttg aattatgtac ttctaagtca
     6901 atttgaattt actcttgaaa attttaatat tataaactta atttctttta cattagttgg
     6961 tttatcgtta attttatttt taaaagataa ttctttgtat aacaaggtta gaaatagaaa
     7021 aatcacagaa gcttttgaag aaaataaaaa caatatactt attgaaaagt gtaagctgat
     7081 actttttgtt gtggttttat ctttagcaat aattgtaact tattgtacta agggatatgt
     7141 actttttaat gtaactatga tgactttgtc tgtattgata gttccaatat ttgaagagtt
     7201 attttttaga gaatatatat ggaactatct aagtaatttt ataaagtcaa agagtaaaat
     7261 tatctgtata acatctatac tatctggaat ttataatata ggatacatag atgttataag
     7321 aaattatgta atattgtata acaattcgag ctacaccttt gaagttatta tttcaaaaat
     7381 tatgataggt actgtttttg gtattgtact tggattagtt aagtataggt ttagagatgt
     7441 agggttttgt atattattga ggagcttatt tgctatattt attaggtaga tagtataatc
     7501 ggtttataag gttgttttaa atttaatttt aaatcaacct tattttattt gtgttttata
     7561 aagcacttat atattaataa ctaataaatt taataaaaat tatttgaata tttttaaaat
     7621 atataaatat gtattgacat ataaatatta tgaattacaa tttatataaa ctaataaaaa
     7681 ttaaataata ttaatcaact tattgaatgg aggaataaaa atgaaatttg tgactttttg
     7741 tagtagcaat gaagaaaaaa taggtgtatt taattcagaa acaaatagta tatatgaaat
     7801 taatagttta ggattaagta aattatatac tgatatgaat gattttattg aaaacgtatc
     7861 tacaggagat ttagaaaaaa taaaaaacaa ttcatttgaa aacgcaaagt gttataagtt
     7921 agaagaagtt aaattatgtt ccccaattgt aagacctaaa aaagatatta tatgtttagg
     7981 tctaaactat aaagaccatg tcaatgaaat cccagatgga gttataaaaa acgtagttat
     8041 gccagattat ccaatttatt tttctaagag agcagacaaa ataattggag tggatgataa
     8101 gataagctta catggagatt tggtagagaa gttggactat gaatcagaac ttgcagtaat
     8161 cataggtaaa gaaggtataa atatatcaaa agaagatgct tatgaatata tatttggata
     8221 tacaatagta aatgatataa gtgaaagagc acttcaagat aaacatgttc aatggtttag
     8281 aggaaaaagc ttagacacac atacatctat gggaccttgt atagtacata aagaagaatt
     8341 tgaacatcct ttaaaattag atataagtag tgtagtaaat ggagaagtaa gacaagattc
     8401 taatacagag tactttatat ttgatatacc tactataata aatgatttat caagaggaat
     8461 gactctaaag ccaggagata taatttcaac tggaactcca gcaggggttg ctatggggat
     8521 gaatcctcaa gtatatttaa agcatggcga tatagttgaa tgtaaagtcg aaggaatagg
     8581 agttttaaaa aatatagttg attaatggat aaatatgcca tataatttta ttagtacgaa
     8641 taatttaatt aatataggat taatcaaact tatgattaat ttattataga gagggatgag
     8701 tatatggcaa gagaactaga attaaagtat ggctgtaatc caaatcaaaa accatcaaag
     8761 atttatatga agaatggaga acttcctata gaggtattaa atggaaaacc tggatacata
     8821 aattttttag atgcatttaa tagttggcaa cttgtgaagg aattaaaaga agctacaggt
     8881 cttccagcag caacttcatt taaacatgta agtccagcag gagcagcagt aggggtacct
     8941 ttatcagata ctttaaagca aatatatttt gttgatgatt tagagctttc tccattagct
     9001 tgtgcttatg caatggcaag aggagcagat agaatgtctt cttatggtga ttttatagca
     9061 ctttcagatg tatgtgataa ggagactgca actattatag caagagaggt ttctgatggt
     9121 ataatagctc caggatatac agatgaagcc cttgaaatat taaaaggaaa gcgtaaaggt
     9181 aactataata ttgtaaaaat agatgaaaat tatactcctg aacctattga aacaaaggat
     9241 gtttatggaa ttacttttga acaaggtaga aatgaaatat taataaatga agacctatta
     9301 aaagatattc caactgataa taaaattttt acagatagtg caaaacgtga tttaattata
     9361 gctcttatta cattaaaata tacacaatca aattctgttt gttatgctaa agatggacaa
     9421 gtaatagggg taggtgcagg tcaacaatca cgtattcatt gtactagact tgcaggtaac
     9481 aaagctgata cttggtatct tcgtcaacat cctaaagtat taaacttaaa atttaaaaag
     9541 gatataggtc gtccagatag agataacact attgatgtat atctttctga tgattatatg
     9601 gatgtgcttg ctgatggcat atggcaaaac ttttttgaag aaaaaccaga gcctctaact
     9661 ggagaaggaa aaagagcatg gcttaaaact ttaacaggag tagcacttgg ttcagatgca
     9721 tttttcccat ttggagataa tatagaacgt gcaaaaagaa gtggggtaag ttttatagca
     9781 caaccaggtg gttctattcg tgatgataat gttatattaa cttgtaataa gtataacata
     9841 gtcatggcat ttactaaaaa tagattattc catcattaaa atactgaaag tataaagata
     9901 atatagatta agtaaaaatc tacaacaagt tttgttgtag atttttttat ttgctgtata
     9961 attttaaata gaactttatt tttagatatt caatttggag gttgatatgg ctttaaatat
    10021 aagaaactat agcctattat gtaatcaagg aacagctaaa tttaatgaag atgtagtcgg
    10081 tataaatcca tttggggcat gggttttaga tggtgctaca ggacttaatg gaaaaaattt
    10141 agtgtcaact gaaagtgacg caaggtggta tgttcagtgg tggaataggt atttatataa
    10201 aaatataaat aaagaagaat cattaaagag tattattaat actggaataa tagaagtaaa
    10261 aaaagagtat gaactaagat taaatgggtt taaggttgaa aaattagatt taccatctag
    10321 ttctatagtt gttgtaaaat ttcatgaaaa taaagtagag tattttttat taggagattg
    10381 tgctttgttt attaaaaatg gagattccaa agttataaaa gataggagta tttgtaaatt
    10441 tgataatact gtatttgatg aaatgagtaa actttctaac ttggaagaga tggcttttga
    10501 tgagattaga actagtgtta tggatacaat agtaagcaat agattgaaga aaaatacaaa
    10561 agagggctat tggatattgg attttgatga ggaagctgtg gaaaattcta tatatggata
    10621 cattgaaata aaaaaagatt ttcaattgat gctaacgagt gatggtttta caagtgcatg
    10681 tgatagatat aatctaataa aagaagaaga attgatacgt atagcagaaa aatttggtat
    10741 aggatatata cataataaag taagggattt tgaagataat gactataaag cggttaaagt
    10801 gccaagattt aaagtaaaag atgattcatc ttgtttatat ttagatatat ataatgatta
    10861 gactgtataa aactatttaa ttttagaaag gaggcaagtg aaaagtgaaa attcttcata
    10921 taataacaca aaaaccaaat agtacaggta gtggtatata tttgtctggt atgataaaag
    10981 ggttcgaaaa aatcggtcat aagcaagctg taattgcagg tatagatgta aatgatgatg
    11041 taaattgttt tccaagtgaa gtaagttttt atccagtaaa gtataattgt ggggagttaa
    11101 attttcctgt tgtaggtatg agtgattcta tgccatatga aagtacaaga tataaagatt
    11161 taaatataga catgataaat agattgaaat atcaatttaa agtaaatata gataaggcta
    11221 tgaatgattt taaaccagaa cttataatat gtcatcattt gtacctctta actgcatttg
    11281 ttagggaaat ggtaaaagat ataaaggtta tgtctatttg tcatggaact tgtttaagac
    11341 agttaaatac cattgattta gagaaagaat acataatagc aaatataagg aaattagatt
    11401 taatatttgc tcttcatgaa aatcagaaat atgatattat aaagactttt ggagtaagtg
    11461 aaagtaaagt agttgtaatt ggtagtggtt ataatgatga tatattttat aataaaaatt
    11521 ataaaataaa ggatgataaa ataaaaatag tatttgcagg taagatatgt aaatcaaagg
    11581 gactgattcc atttataaaa gctatatcta aattaaaata tagtaaagat ttaatagaag
    11641 tcaattttgc aggtactgga agcgatatcg aatcatataa tgagatagtt aaactggcaa
    11701 gtaagtctcc atttaaaatg aatttcctag gaaagttaga acagagagat ttagctgagc
    11761 tattcaacag gtcacaaata tttgtgttac catcatttta tgagggatta ccagttgtag
    11821 ttttagaagc tttatcttgt ggcacagatg taatcactac tgatattctt ggtgttaaag
    11881 agtggattgg tagtgagatt aataattctg gaaagataga atatgtttct ctacctttta
    11941 tggagaaaga aggtattcca aaagatgaag agttatatga ttttgaaaat aatttgtata
    12001 atgcaataga ttctaagata caatctttat tgaataactc aaataagaaa acatctgtag
    12061 atatgtcaaa aaaaacgtgg gatggcttag catatagaat aaatgaagtt attttaatgg
    12121 atgaattgtg tttagtctaa aattatttaa attataaaaa agtgtaacta tttttacact
    12181 atttttaaat gattttatat tatactaata ctaaatatat ttgttttagg gaggtaattt
    12241 atgaggatta gtaaaaaggt attggcgtta ggtatttcag cattactgtt aagtgttagt
    12301 tttccaacat caataaacgc actagataag attgaaaata ttcaaggagt agataaatat
    12361 gaaacagctg ggcttatttc agataagcaa gattttacaa cagcagttct tatcaatgca
    12421 gactctacaa tggtagatgg gattgctgca agtggtcttg caggagtaaa taatgcagct
    12481 attttattaa ctaataagga tgatatacca gaagcaacac ttcaaagact gaatagagtt
    12541 actaagatat atgtaatagg tggagaaaat tctataagta aagatgtaga aaaaatgtta
    12601 cttatgagaa gaatgcaagt tataagaatt gatggtgtag atagagtaga tacaagttat
    12661 aaaattgcag gtgaaataga aaaaattaaa aacagtgata agatgttttt agtaaatgga
    12721 tttaaggatg aagctgatgc agtaagtgtt gcatctgttg cttatagaga tggagctcct
    12781 ataatattga caaaagatat accttcagca gaagaagaca cagacttaga cccatggttt
    12841 ggagtttctc ctgttccagt atatgctatt ggaggagagt ctacattaag tgattacata
    12901 gtgagcagat atagagcaac tagaattggt ggtgttgata gatatcaaac taataaaaat
    12961 gttattgaaa agttctataa tggagcaaaa gagttctata taacaagtgg agatgattta
    13021 gtttatgctt tagttgcatc accacttgca aaaaatgctc ctgttgtttt agtttcaaat
    13081 aaaagtgata agagtatatt aagtggagct agtaaagtaa cagcaatagg tataagtgat
    13141 aagagtatta tagagcaatg tttggatgct gtaaagaagt aatggagtgt tattagtaaa
    13201 atgaatatat acattgaaaa ttagtagatt gttcatttta taatggtata aaatatatgg
    13261 gaagaggcta tcaaatggag ataagcctct ttttcattgt taaaattatg aaaagatact
    13321 ttattttata taaaaaagta tcttatgaac taaatgtcta ttttgagaca ctatctactt
    13381 ttcttatatt atattatttt ttatatggaa taaatttcat aagtattatc ttcctttaat
    13441 ttttctaacc aaaccatctc taaaatatct agttcttcta aatagtcttt ctctgttttg
    13501 ttatcatcat gttttataag ttctaaaact tcaaatgtaa atgctttttc accatatttt
    13561 ttccattcat tatttaattc ttttatcata catgagccag caccaagctg aaatttgaga
    13621 gagttaaatt tgctttttat gtcattactt tttccaaggt aaatcttttt agtaatatca
    13681 ttttttatta tgaatacacc tgtgtcaaat ttcatttctt tgtataattg ttttaattca
    13741 cgttttctat ccatgatttt agatacctct ctttataatt gatatgtaat atgtaagtgt
    13801 aaaaatatat tacatatcaa ttataaagag attaatttta tatgtaaata gtttttataa
    13861 atttaattat gaattagtca tcaataaaca agaaatttag ctgtaaaaat ccattataaa
    13921 ggctattata aattttaata tgctaaaaat ataaaaagaa tagttgacag tgtagaaaat
    13981 atggaataaa ctaaaagtgt aatagtaata tgaagttata tattacattg aaaggtgtga
    14041 atgattatta tgaataaaaa aatagaatta atagggaatt ttgcaagtga ttggattagg
    14101 tatagtgatt atgaatataa aattactgaa gaagatgaat tatatatagt tccaacagaa
    14161 gatgctgtgt ttaccatgta taacccattt gatgtagcag atgatataat agtagatata
    14221 attcgcatag gacaagaggc attacaagat atatcaaaag agacaaaaga aatgaaacta
    14281 aagaaagaag tacttgaatt tgtaaaaaag tatggattat tgggtttaat atattcaagt
    14341 gtatataata gagatatagt tggagaagaa agagtactga tgattgaaaa gaattatatt
    14401 acaaaagaga atatattgag tacagatgaa tatgtaaaca tgtttatacc atttgcaagt
    14461 gatgaagatg tgtattttaa aaagtataga aggggtatag atgtatcaaa gagagaagaa
    14521 tctccaaagt tttttggaaa gagaccattg attttagatt tagtattttc aaagttctac
    14581 actgaacaac tagattggat tgtaagtttt gctaaagata tgtgtaagca ttttaatcaa
    14641 atttgtgtat ataaaaatac atctaatcta ttaactgaaa gtgttactat tatgtctgga
    14701 gggtttaatc ctcaaaagat aggattttct ataagccaat tagataaacc aattattgca
    14761 tgggagtttg attctttgaa aacagctatt gagactatct atgcctttgc aattacaagt
    14821 gaaccttctt ctttaaataa atgcaagtac tgtggtaaaa tttatattcc tacaaattct
    14881 aaatctcaat actgttcacc ttcttgtaga aattgctcaa atgtaaagaa aagtagaagt
    14941 agagccaaaa aagataaata aaattaataa atatatcatt gtttagattt tagtaaataa
    15001 ctactgaatt aaaagttgtt atagaaaatt atatatctaa atgatggtat ttttttgttg
    15061 agaaaattga aaagtttaat tgagatagta attttgagtt gacaaaaacc acttttaaca
    15121 ctactattat aagtggttgg taaaataata tacattaatt taagtatagc ataaaaaaca
    15181 aaaaaagtca atggaaaaaa taaaaaagga gaatttaaaa atgatatcac ctattatttt
    15241 gagttctata aatcagaatt taaaggaaat agaaagaaat gaactgcttg aaactaatat
    15301 agaatctgga gattatggtc tagctctttc tgaatctgat gtaaaagata taataaattc
    15361 gagagataat actttaaagg ggtatggaag aatagaatta gatataaaag ttacaaaaca
    15421 gttaatagaa aatatatata catcacaata tacaaatgtg gacaattatt tagaggctat
    15481 taatgatatg caagaaattt tttattattt aaaaaatgaa actgatgaca agatatgtga
    15541 tgatgagatt atagaaatac tgggtgaatt ttatgaaaaa ttttcaggca atatggacaa
    15601 tgtaagagga gaagcagacg aatttgcaaa gaaatttaaa tttggagaag tttaatatat
    15661 gataggagag attttgtatg ttaaataatt taagtaatat taatataaac gaaaataatt
    15721 taataaagaa ccaatattcc atatctctac ttaaggaatg tctaaattgt aaagtgatag
    15781 atgaaagaga agtatataat atacaacaag aagtatctct aatacttatg gacttgatta
    15841 aaaaatatac aaatggtcaa agcacatcag taaaaacaga agttgccgaa aaactgttga
    15901 tttcaatatg gtatgctatt gatgcatata taaataagct tggagatata gaaaaaagag
    15961 ttgtggagtt gactgctaag aatgttaaaa aagtgtacct tggaggcatt gaaatattag
    16021 aggaagagtt agttaggcta aaagattttt ataaactaat gatgagtaga aaattggata
    16081 ctaattcaat agcatataat gataccttga tggagttatc aagtttcttt aaaagttata
    16141 ttgtaaaatt tgaagctcag gacgttccag taagtataga ttatccattg gcactagatg
    16201 atatggatgt taagggtatt tattatgtga agaattatat tgagagtata gatatagaaa
    16261 atagattttg taacctattt aagaaaaaag atagagaaaa attattttat gactatggga
    16321 tgacttataa aatagattgt gatgttatac ttgtaaattt atttgaactt attatgaata
    16381 attgtatttt ttctactatt ttaggaaatg aagtgactaa tttagaaatt tcaaaatatg
    16441 agtatgaatt tttagaaaaa caatttaaaa atatgtacaa taatattact gaagataaag
    16501 ttatagactc tgaagaaagt aatataattg aattgattga agtagatgat agttttaaca
    16561 ctgaagtaga tagtaatttg gattataatg attttgaaga aattaacaga gaaattagaa
    16621 taaataaaaa aataacttta ctacttatgg aagcaattga agtattaata aaaagattaa
    16681 gcatagaaga tgaagatgta ataagatata ttaaaaaata tgaaaagata tttattgaat
    16741 ctgtgctaag ctcaataaaa agtaacactt taaaaagtat ggtaactgtg agtaaaaata
    16801 aaaatgaaca cttagaagat tatattgttg atgatgaaag taaattggat gatgaatcgt
    16861 ttagagagat attcaatgag atattagata gtacatctat aattgaaaaa ataagaataa
    16921 taaaggaaaa tatcaatgct aaaaaagact ttatcgatat tcttgaatct gaatgtttat
    16981 ttggagaaga gtatttaatg ttgtttgcat cattaagtga attagaattg gcaatattgg
    17041 gtagtgtagt attttatgaa gatattagaa tgagagaaat taatatatta gaatttatgt
    17101 taaatgagaa aactgaaact actttatgga agatagagta cattaaattt atgaagaggc
    17161 aaagtgaaga taagatatac tccatagaag agcatatgaa taaaattaat taacaaaaaa
    17221 ttgtgaataa atagaatgtt agtatataat ataatctgta aagcatatca aaaatacata
    17281 tatttggagg aaaattatga aagagtatat cataaataat gttgaagaaa taagagaaga
    17341 attaattgat ttatctaaga aaatatggga aaatccagaa ttagcctttg aagaaaagta
    17401 tgcaagtagc attcaaaaag aatacctaaa gtcaaaagga tttaaaattg aagaagtaga
    17461 aaatttacct actggattta tagcaagttt tggagagggt aaacctgtga ttggcatact
    17521 tggagaatat gatgctttac cagagctatc acaatgtgta agtgcagaaa gaaagccatt
    17581 ggtagaagga aaagctggac atggatgtgg gcataacctg ttgggtgtag caggtgtagg
    17641 agcagttgtt tctataaaaa aattaataga agaaaataaa tttaatggaa caataaaata
    17701 ttttggttgt cctgctgaag aagaaggtgg aggaaagaca gttatgtgta taaatggctg
    17761 ttttgatgat gtagattgtg catttacatg gcatcctttt gatataaatg caccttggag
    17821 aggtggaagt ctggcaaact tatctgtaaa atttaaattt aaagggataa cagcccatgc
    17881 agctcaagct cctcataatg gtagaagcgc acttgatgct gttgaaatta tgaatgttgg
    17941 agctaattat ttgagagaac atgttattga ctctataaga atgcactatg ttataactaa
    18001 tggtggtgga agacctaatg tagttcctgg atttgctgaa agttggtact ttattagagg
    18061 taaaaaagca aaagatgcag aacacgttct tgatagatta ataaaagtag ctcaaggagc
    18121 agcaatgatg acagaaactg aaatggagta taaggttaca gatggaatat atgactatat
    18181 accaaaccaa tgtttgactg atttagttta tgacaatatg gtttttattg gttgccctaa
    18241 aaatactcct gaagaagaag aatttgcaaa aaagttgtgt gatactctta ctagagaaga
    18301 aagattaggt gtagcaacat cattacaaaa tgataaatct attgtagaaa gctatataca
    18361 caaggaaata gttgacggtg acaaagataa aggactagct ggttctactg atgttggaga
    18421 tgtaagttat gtaataccag tggctcaatt tgcaatggca gcttggcctg ttggaattgc
    18481 aagtcataca tggcagtcat gttcatcagc aggttcaaat ataggatttt ctgctatgat
    18541 aaattcagct aaagtactgg cttgtagtgc ttatgatgta tttatggata ctaaaattat
    18601 tgatgaagca aaaattgaat ttgataaatc attagatgga caaaaattta aaccattggt
    18661 ataattttgg taataaaata tttcaggggg gaatagctat gagagaaaaa gttgaaaaag
    18721 tgcttgagga aaagataaaa ccagtattac aaagagatgg tggagatgta gagcttatag
    18781 atgtaaatga aaatggtgtg gttttagtta gactacaagg ggcatgtagt gggtgtccag
    18841 gagcgactat gacaatcaaa gctattattg aaaatgtttt ggtgagtgaa gttccaggag
    18901 taactcaagt attaggtgtt taatagttag cgtttattaa atgtatgtta tacataagta
    18961 tttaataaag aagtaaaaaa aatagtgaaa caatatatat gaaaaagtat gagaaagata
    19021 ataaaacatt atagaaataa tgaaatattt catatgtata tgaagaaata agaaaaatag
    19081 tgaagcaata tatatatgaa aaagtatgag aaagataata aaacatttat agaaataatg
    19141 gaatatttca tatatacata tgaagaaata gtaaaacaat atatatatga aaaagtgtga
    19201 gaaaataata aaatactata gaaacaatta aatattctat ataggtatta aggaacatga
    19261 aagaaataat ggagtattat aaataaatat gtaaaagtgt gaaagaagga tgtaggtaaa
    19321 tttgtcttat acaaatttac ctacatcctt ctttcacact ttttatttta ctatccagcc
    19381 ataccaataa acatacctat aaaatatgga actaaccata tagtccatac aactatacta
    19441 aacttatgaa agaatttttt cttttcctca tcatctttgt ataggacaaa agtagcccaa
    19501 cttgcatgaa aaagcataag cacaattgca agaagacctg taatactatg aaggttttgt
    19561 gataaagcag attttacttc aaaagtatgg ctgttggcta tgttactcat tgtaaatgtt
    19621 cctaaagtat caaatataaa accaagccaa aatataatta catgtttctt ttttaaaatt
    19681 tttgcctttc tttctccaaa tacacctata gtgtagaaaa ttaaagctga tgttataaat
    19741 actattgcaa gtattaattt ataattcata attatctctc ctttactttt gccaatatat
    19801 ttctaagatt ttttactaag tcactcaatt cgttttcatc acaattttta aaaatatcat
    19861 caaaactttc ttgcatttta attttaaaat ttgtggcaaa ttcaaatcct gttgttgtaa
    19921 acttaacgta tgtatttctt ttatcattag atttcttgaa tttttctata tatccaatct
    19981 gttctaatct gctgattatg cctgaaaccg tacctttagc taaagacatt tcatcacata
    20041 gttgtgatat tgtaagttct tgattatgag ctatcagttt tataactata atctgttggt
    20101 gtgttaatcc attttcttta aaattttctt ctactgtgta catagtttta gaataaagtt
    20161 cttttaagag catggctatt ctaaaacagt cataattact tttcaataaa atcacctctt
    20221 aaatggtttg catacaaact aatataatat acttttctta tatagtcaac ctaaaatttt
    20281 ataattttat agaaaataat gaagaataga atataaaaat tttattttag gtataaataa
    20341 aataatttga tgaaatttta acaattttta aaaagtttgt ttacacagtt aataaatgat
    20401 gctaaaatta actcatagat aaatatagaa aatttaattt tgtttaatga taatggaacc
    20461 acgaaggttt taacgttact ttacgtttgc ttttatgtgg ttttttttat tgcaacaaag
    20521 gggggttggg gtttgttaaa ggatatactg aaaaagttgt tgcagtttat tttagtaatg
    20581 tttttattat cttttattgt tttttacatg gcaagacttg ctccaggaga cccattaatc
    20641 tcttactatg gggatggagt agaaagaatg agtacacaag aaaaagaaaa tgctatgaaa
    20701 aaacttggtt tggatgaacc aatatacagt caatatataa aatggatttc aaatgcatca
    20761 aaaggggaat ttggaatttc atttaaatat aaacaaaatg ttacttctgt tataaatgat
    20821 gtctatataa acactataat attaggtggt tcaggatata tacttacctt tgtgctagcc
    20881 ttattgttag gaatattttg tacattacat gaagataagc tcattgatag aatcatatgc
    20941 aaattaggaa caataaccaa ctgtattcca tcattttggg ttgccttagt actcatactt
    21001 atatttagta taaacttaag tatacttcca agtagtggtg cttattcaat gggagaagag
    21061 tccagtatat taagtaggat atcacatcta atcttacctc ttacagtact tatattaagc
    21121 catctatggt attacacata tatgataaga aataaattac tagaggaaat aagagaagat
    21181 tatgtgcttt tatgtaaggc aaaaggttta aataatagaa ctattgtttt taagcattgc
    21241 ctgagaaata taatgccatc atatattagt ataatggcca tttcgatacc tcatatacta
    21301 ggaggaactt atgtagttga aaaagttttt tcttatcctg ggctaggaac actttgtttt
    21361 gaaagtgcaa agtatcatga ttataatatg ttattagttt tatgcttaat aacaggggct
    21421 ttagtagtat ttgggaatat gttagctcaa attataaaca ataaaataga ccctagaatg
    21481 aaatatgata gaggtgatac taatgaagcc acaatatagt gattttgaaa ttgtaggaga
    21541 aaattatatt ttagtagcag aagatgaaca tatagaaaca aagaaaacct tatctaaaaa
    21601 aattaaagaa ttaccatata tatcaattat tattttatca atcatagtca ttggaagtgt
    21661 attctcatct tttataatga cacatgaacc aacatacatg gatttagcaa gttctaattt
    21721 agctccaaat aaaacttttt tatttggaac agactcaatg ggtagagata tatattctat
    21781 gatatggtat ggaggaaaaa tatctttatt tataggacta ttttccactg taatatcaac
    21841 tgttatagga attgtttatg gtagtataag tggttcagca tctgaactcg tagatgatgc
    21901 aatgatgagg tttacagaaa tcatacttag tataccttca atattaatta taatatttgt
    21961 acaagctata cttggaaatt caaatccagt atctatgtcc atagtaatag ggataacaag
    22021 ttggatgaat atatctaaga tagtgagaac agaagttaga caaataagaa atagcgaata
    22081 catactagca gctaagagca tgggaggagg attcttctat gtattaaagc aacatttact
    22141 tccaaatttt gtggcatcaa taatgttcat ggttgtaaca aacataggcg cagctattgg
    22201 tactgaatcg acacttagct ttttaggaat tggtttacca atagaaattg tttcttgggg
    22261 aagtatgtta tctttgtcag aggaagcatt actttcaaat agatggtgga ttatactgat
    22321 tccgggtata tttttggtta ccacattggt gtgtataaca aatataggta attatataag
    22381 aaagaataat aataaaaaat ctagtaattt ataaatcagc atttgagtat taaataagca
    22441 aatactgagg tatttgaaat atctatttaa ataaatgggg tcaaagcttt gaaaaatatt
    22501 ttataaaaag caaaaggggg agcatcatga aattaaagaa gttaaaagtt ttaagtttag
    22561 taatgatact tagtctaatg gcaggttgtt ctagtggagg agacaaagat aagaaggcag
    22621 atacgcctaa agatggaaag gtacttgttt atggaagtaa tgactataca agtataaatc
    22681 ctgcactata tgaacatgga gaaataaatt cgttaatatt taatggatta acagctcatg
    22741 atgaaaataa taaggtggtt ccttgtctag caaaggactg gaaatttgat gaggcaacaa
    22801 atacatatac ttttaattta agagatgatg ttaagtggca tgatggagaa aaatttacag
    22861 caaatgatgt taagtttaca atagaaacta taatgaatcc agataatgct tctgagatag
    22921 catcaaatta tgaagatata acaaaaattg atgtggttaa tgataatact ataaaaatta
    22981 ctttaaaagc tccaaataca gcaatgcttg attatttaac agtaggagta ttaccaaaac
    23041 atgctttgga aggtaaggat atagctactg atgaatttaa tcaaaaacca ataggtactg
    23101 gaccatttaa acttgaaaaa tgggacaaag gtcaaagtat aacacttgtt aagaatagtg
    23161 attattttgt aaaagaacca ggattagata aagtagtctt taagattgtt ccagatgata
    23221 aggctaaggc aatgcaatta aaatcaggag aattagacct agctcaaata actcctaaag
    23281 atatgtctaa ctttgaaaaa gacgaaaaga attttaaagt aaatataatg aaaacagccg
    23341 actatagagg tatattatac aattttaatt ctaaattctt taaagataaa aaagctaaag
    23401 gattaccaaa tgctttaagc tatgcaatag atagaaaagc aatagttgac agtgtattat
    23461 taggacatgg tgtaccagca tattcacctt tacaaatggg accatataat aatcctgata
    23521 tagaaaagtt tgaatataat ccagaaaaag ctaaacaaga aattgaaaag ctaggttgga
    23581 aattaggttc tgatggaatc tatgaaaaag aaggtactaa gttagctttc gagataacag
    23641 caggtgagag tgaccaagtt agagttgata tggctaaaat atgtgcacaa cagttaaaag
    23701 aaattggtgt tgatgctaaa gctgttgttg taactgaaac agattgggct aatcaagatg
    23761 ctcatttaat aggttgggga agtccatttg acccagatga ccatacatat aaagtatttg
    23821 gaacagataa aggtgcaaat tacagtgctt actctaatcc tactatagat aaaatacttc
    23881 aaaaagcaag agaaacagaa gataaagatg aaaaattaaa gttatataaa caattccaag
    23941 tagaaatgac taaggatatg ccttatacat ttattgctta tatagatgca atatatgttg
    24001 gaaaaccaaa cataaaagga ttaacacctg atacagtttt aggtcatcat ggtgtaggta
    24061 tattctggaa catagctgac tggacaattg aataatactg aaattaatta gaaaccttag
    24121 ggataaaaat atatccctaa ggtttgaata gcataggggt tgatggtttt tggaacattt
    24181 attggaggtt aataatctat ctgtaagttt taaagtagaa gaaggagaag ttcaggctgt
    24241 aagaaatgta tcttttaatc taaaaaaagg tgagacattg gctatagttg gagaatctgg
    24301 ttgtgggaaa tctgtactgt gtaagagttt gatgaggata ctcccataca atggatatat
    24361 taaaaatgga gaggttttac ttaaatcaag tgatttggtt aagaaggctg aaaaagagat
    24421 ggaagatata agaggaaaga atatatcaat gatatttcaa gaccctatga catctctcaa
    24481 tccaactata tcaataggaa agcaaattgc agaagctgta atcattcatc aaggtataag
    24541 taaaagtgaa gcaaaaaaga gagccataga gcttattgaa ttggttggaa ttgataatcc
    24601 tgagaaaaga tttaaacaat ttccacatca tttttcaggt ggaatgagac aacgtatagt
    24661 aatagctata gcacttgcct gtaatccaga tgtattgatt gctgatgaac ctacaactgc
    24721 tttagatgta actatacaag ctcaaataat tgatttaata aaggatttac aacataaaat
    24781 aggtttgtca ataatattta taactcatga tttaggagta gttgcaacta tagctgatag
    24841 aatagcagtt atgtatgcag gaaagattgt agaaatagga actgtagagg atatatttta
    24901 tgaccctaga catccatata cttgggggtt gttgggttca ttacctactt tagattcgca
    24961 ggatgactat ttatataata tacctggaat gcctccaaat ttattgaacc caccaaaagg
    25021 agatgctttt gcaataagga ataaaaatgc acttaagata gattatgaaa aagagccacc
    25081 aatgtttaaa attaatgata cacatagtgc agcaacttgg ttattgcacc cagatgcacc
    25141 tgaagtagat gtaccagtta gagtaaattg tggaagggtg atttctaatg aatagagaaa
    25201 aaatactgga aataaaaaac ttaaagcaat attttcattt ggacaaaagt actactgtaa
    25261 aagcagttga tgatataagt tttgatatat ataagggtga gatatttgga ttggtaggag
    25321 agtcgggttc aggaaaatct acaactggta aaactataat aagactacat gaatctactg
    25381 gtggagaagt aatttacaaa ggaaattgca tatcagataa aaaaacgtac aaatttataa
    25441 aaaaagatgt aaacaaaagt atgcagatta tttttcaaga ttctacttca tcattaaatc
    25501 cacgtatgac aattgcagat ataatttcag agccactaaa aattcaaggt atctgtaaaa
    25561 ataaaacaga tagaatgaat aaagtatatg agatgctaaa gctagtagga cttgatagaa
    25621 gttatgcaaa taagtatcca tctgattttt caggtggaca aagacagcgt attggcatag
    25681 cgcgagctct ttcagtagac ccagaattta taatagctga tgagcctata gcttctttgg
    25741 atgtgtctat acaagcacaa atagtaaatt tatttaaaaa attgcaacaa gaaaagaatt
    25801 taacttgttt atttatagct catgatttat ctatggtgag acatattagt gaccgcatag
    25861 gagttatgta taatggaaaa ttagtagaat tggcagattc aaatgaattg tataataatc
    25921 ctattcatcc atatacaaaa tcactacttt ctgcaattcc tgttcctgac cctagatatg
    25981 ctaagtcaag aaatagaata gagtataatt ctaatggata tgattgttca aatgaaaagt
    26041 cattaagttg gattgaggtt tcagatggac attttgtata ttcttctaaa tcagaaataa
    26101 ataaatatca acaaaattta aaagttgttt agtataaaaa tacttgaaca ggtatttatt
    26161 tgaaatacaa actaaaaccc tatttttgga gaaattttaa aactactcca aaatagggtt
    26221 ttttatttct acttttcaaa tataataaat tcaaatataa taaaattaaa aacaagtaaa
    26281 gtatgttgtt aagaaagtac tataaaatta aaaatagtaa tgtagaaaaa atgagcaata
    26341 atcttatttt ttctacaata attttctata atttaaagta aggaaagttt gataaattat
    26401 aaccttagtt aaagaataag agtttattaa aaaataaagt gtgtataggg gttttagtta
    26461 ataaaatact ataatgtagg ggagcttgta atgatatata aaaaagataa agtaatgaaa
    26521 gttttagaat catcatatag ggaaaatgaa aaagggatta cagctaacca attatcagaa
    26581 aagacaggaa ttgcaagaaa caatataagt acatatttaa accaattatt caatgagggt
    26641 atggttacga agataaaagg aagacctgtg tattatatcc ctgcaaatct taaccttaac
    26701 aagaatgaat tgcttgatac acaaagtgag ttgcttggca taaaaaaagg aacaaaagta
    26761 gaaaacaaaa taaaaaaaga tagttttgaa acactaatag gtaaagaaca tagtttaaga
    26821 cctataatag aaaaatgcaa ggtcgcaatg ctctatccta caaatggact tcatactatt
    26881 atctatggag aaacaggagt aggaaagagt atgattgcta gatatatgta cgattattct
    26941 atagatagtg gaattagaaa aaataaagct ccttttgtaa catttaattg tgctgattat
    27001 gctaataatg cacaattact tatgggacat atttttgggg tagaacaagg agcttataca
    27061 ggtgcagaat cttccagaaa aggtcttttg gaaattgcaa atggagggat actattttta
    27121 gatgaaattc atagactacc agcagaaggt caagaaatgt tgtttacttt tatagataag
    27181 ggaacattta aaagactagg agatgcaacg aaagaaagag agtctaaggt attaataata
    27241 tgtgctacta cagaaaatcc gacatcaaca ctattagata cattcaatag aagaatacca
    27301 atgaaaatag aaataccatc actgagagat aggacaatga ttgaaagaat ggaattagtc
    27361 aaaaattttt tcaaggaaga atcaaaaagt atttctattt caataaaagt acataaagaa
    27421 ataattaaat cactattact ttatgattgt aaaaataatg taggtcaact tcaaaatgat
    27481 ataaaattag ctgttgcaaa tggataccta agatataaaa gagataataa agaaccagtt
    27541 tatatagaga aaaaatattt tgataaatct ataaatgaag tgagtgatga ttatagagag
    27601 aagtctatta aagcagatga agttataaca catggaatag aatattttgt atttacgtca
    27661 ttaggagaag aagaagttat taactacaat gatataaata ttattaatgc agtaaatact
    27721 aatcttaata aatctgtaga aatgaatata gcagatgctt ctctatttaa taatgttaag
    27781 tttaaaagca tgtgtgaatc aataagacaa attattaaag aagatgaaga aatagatata
    27841 agtgaatcaa tatttaagtc aatggcaatt tatataaaaa gcgtattgga taaaagtagc
    27901 tctacaaaca acatagattt gaatcaaatt agaagaaata acagaagtga atttaaaaca
    27961 gcattaaaaa tagttggaat aattgaaaat gaatttgatt tatttttatc tatagaaact
    28021 gtagcatata ttacattatt catagtacaa gctaaacaag atgaacaaaa taaaatagtg
    28081 aataacatta atattatagt tgctatgcat ggagaaacta cagcaagtag tatggtaaaa
    28141 gcagttggtg atattattgg aaagtgcagt gctatttctt tcgatatgaa gttagataaa
    28201 agctatgatg aagttattgt agattttaaa aatttaatta ataatataga caataaagat
    28261 gtattactat ttacagatat gggttcatta aactcttttg atgaaattat aaagaaagaa
    28321 aaaaagtgtg gagtaagagt aattcctatg gtgacgacat taactgtgtt agaagcagtt
    28381 cagaaggcca atatgggatt acctttaaat gacgtataca actctatcac taacacgaga
    28441 aagtactatt ttggaactaa tgaaattcaa aataaagaaa atctgtctaa aacaattatt
    28501 atagcatcac atgtttcaga aggtgtagat aataaaacaa gaaaaatatt agaagaaaag
    28561 atgagtaggt acttagatgg aatagatatt atatctgtcc catataaaac agaaaaagat
    28621 ttaagtttaa atattacgaa acttaaagaa agtagtaata ttgttgcagt aatcaatgaa
    28681 caaagaatta atattagagg aattgactat attagcaaaa aagatataga taaggatgaa
    28741 aatatcaata aattaaaaaa tattataaag ataagtatag gttatgatga tgtagttgaa
    28801 ggtttaaaga catccttaaa aagctctaat tataatagga tatttaaaga tataaaatat
    28861 gtgtctgatg aattgttttt agtatttaat atagaaaaga aatacgataa agtaattgga
    28921 cttatgatgc atttagcttt catggtagat gggttgattg gaaatactag agaaattgaa
    28981 aagcttgata aagaaaaaac acttgattat cacaagagtc taagcaaaat aaaagatata
    29041 gtatctcaat tagataaaaa atataatata gaaattaatg aaaaagagtg ctatcaaata
    29101 ttattaatat tagaatatgc agaaataatc gaaaaagatt accaatgagt ataaaaactt
    29161 atttggtaat tttttttagt ctaaaataat attaatatta gacacatata aatatactta
    29221 gacaaagtat ctaagttttg atgataaaaa tgattgaaat aacagtgttt taaagtgtct
    29281 aaaaaaactg gcataatact tgcttatatt attaaatgag caatataaat aattaaaatt
    29341 taaataaaaa ggagtaaata atatgcaaga taaaatatta agagataaaa aggcaaatga
    29401 cccatgggca agaatgacta caagaaacgg atttagtgca gacgaaataa tatcgtcatt
    29461 tcaaaaagct ataagaagaa atatggtgga agaagcttgt gaatttgctt atgaactata
    29521 tatatcatca ccagaattag aagataagct atggagaagg atacttacaa tatcagttga
    29581 agatatagga atgggggacc catcagctgt aataatcata aataatttaa atcaagtaag
    29641 aaaggaatat tcatatgcag atggagacag acctttattt ttcatacatg caataagata
    29701 tctttgtgca tgtgagaaag atagatcaag tgatttatta aaaaatataa ttataaaagg
    29761 atttgcaatg ggtaaagttc cagaagttat ggacgtggca cttgacaagc atacatatag
    29821 aggattagaa atgggaaggg attcattcca ctttttaaat gaagctagta tagtaatacc
    29881 agaaaaagaa gtagataatg attataaaga aagatactta aaaatattag aaaaatataa
    29941 gcaagaagaa gttatagata cggctttcaa gtttaatcat tggcagtact aagaattaga
    30001 gtaaatatta ataagaatta aaataaaata ataagaatta aagtaaaaac tgataggaat
    30061 tagagtaaga attggctgat aaaaaattag aaaacaaaag aggttagata ttgtaggagg
    30121 gtaattatga atattttatt agtatgtgca aatggagctt caacaggtgt acttgtggaa
    30181 aagatgaaaa gtttttgtag tgagcatgaa aagttaaaaa ctaaaactat aaatatagag
    30241 gcaacatcat ttgaaaattt aaaatcatac attgaagcta atgatacaga tgtagtactt
    30301 gtagcaccac aaattaggtt taaggaagat gatgtagttg aagcttgtaa gaattacaaa
    30361 atagcagttg gtttaataga tactaagcat tatggtcgta tggatgctcc ttctgttatg
    30421 aagtcggcaa tcgaattata taaaaataga taattggggg gaataaaaat ggaagcactt
    30481 caaaataatt taagaagatt tcttcttcca atagcacaaa agattgaaaa acaaaggcat
    30541 cttcaagcta taaaagaagg tatgataagc attaccccaa taataattgt tggttcttta
    30601 agtatgttat ttatggcatt aaacaatatg ttacctgaag gaagtgcaaa aacattatta
    30661 tctgaaaata tggatacatt acttatccct aataaattta ctatgagttt attatctata
    30721 tattcagcat tttttatagc tcaggcacta gctaaaaaat ataatcttaa tcatgtagaa
    30781 ataggtatga cagcagttgt tgcacaactt gttgtatgtg ggcaagtagt agatggtgta
    30841 ttagatacaa gttatttaga tgcacaaggt ttatttgtaa gtatattagt agcactttta
    30901 gtagttgata ttactaagtt tatgaatgat aaaaatctcg taattagatt tccaaaagaa
    30961 gtacctagtg tagtaaataa aagttttaga aacttaactc caatgatagt atgtataatg
    31021 ttatttacag caatagcagc aataactaaa aatgtctcag gtcaaccact accagctatt
    31081 ataatgaatt tcttagcacc agctataagc agtgtagata atgtttttgc agtaactata
    31141 atactattta taactcaatt attatggttc tttgggcttc atggagcagc aataacttca
    31201 agtatatgga tgccaatagc agctacatat atggcagaaa atgcaacact gatagcagca
    31261 ggaggagacc caaaatatgt gtttacaata ggtttttact atggtttctt gcaagtaact
    31321 ggttcaggga taactttggg attagtgtac cttatgtcaa gaagtaagtg taagagtttt
    31381 aatagtatgg gaaaagttgt gattttacca tcactatttg gaataaatga gccagtaata
    31441 tttggaacac caatagttat gaacccatat atgtttgtac catttgtatt tggacctgta
    31501 ttggttggag cacttaattt tatggcatta aaagtaggat tagtaggatt acctattgca
    31561 gaaccaccag gctttttacc tccaggggtt gcagcatttt tgatgacatt agattggaag
    31621 gcaatcgtat tagtatttgc aagtataata cttatgacct tgatatatta tccattcttt
    31681 aagataatgg agaaggaaga attaaataaa aatgcagaat tagctacaac attagatgat
    31741 gatagctttg atttttaaca agtcattaga aggagaagga tatggtacct gaaatggaag
    31801 aaaaagtatt tagtataata tcagaagcag gagatgctaa aagtgatgta atgatgtctc
    31861 ttaaggaaat aaaaaaaggt gactacaata aagcaaaaca ccttttaaat tcagcctcag
    31921 agaaaataca aacagcatca aagcatcatt tagaattact atcaaatgct atgaatagtg
    31981 aagatagtgg gactgatttt ttagttgtgc actctgaaga ccattattca aatgcattat
    32041 ttgctcattc tttagtatct gaacttgtag atatatttga gatgatggac ccaagaatta
    32101 ataagaatta aatttcttgt aaatagtgct tgaagtttgg ctatagttta ttaaatcaat
    32161 agatttaata aatgaactcc cttttacttt aaagtactag tatttataaa tttatttata
    32221 aatacagtaa atttttaaat agaaaaggag atattattat ggaaaaattt tcagtagtaa
    32281 tagctggagg aggaagtaca tataccccag aaatcatatt aatgttgcta gataacttag
    32341 ataggttacc attaagagca atcaaattat atgataatga tgaagaaaga caaaataagg
    32401 tagctaaagc ttgtgaaata ctaataaagg aaaaggaccc taatatagag tatttagcaa
    32461 caacatgccc taaagaggct tatacagatg tagatttttg tttagctcat ataagagttg
    32521 gaaaacttga aatgagagaa ctagatgaaa agatacctct aaaacatggg gtagttggtc
    32581 aagaaacttg tggaccaggt ggaatagctt atggaatgag gtctatagaa ggtgttcttg
    32641 aaaacataga ttatatggaa aagtattctc caaattgttg gatgttaaat tattctaatc
    32701 cagcatcaat aatagcggaa gctgttagaa ggttgagacc taattctagg gtaataaata
    32761 tttgtgatat gccaataggt atggaacata atatagcaag gattgcagga ctcaaatctc
    32821 gtaaacatat ggatataaga tactttggat taaatcactt tggttggtat acttcaatta
    32881 aagataaaaa aggaaatgaa cttttaccaa aattagtaga acatataaaa gaatatggtt
    32941 ttataaatgg tgaagaagga atgaaaaatg acaagaaaga tagttggttt gaaactaatt
    33001 tatttactaa agaaatagta aaaacagatt taactacaat accaagtagt tacttaaagt
    33061 actatctatt tccagattat gttgtaaatc attctgatgt aaattatact cgtgccaatg
    33121 aagttattga tggaagagaa aaagaagttt ttggttcatg tgcattgata gaaaagcaag
    33181 gacattctaa gggcagtggg cttaagatag gaatacatgc agagtttata gtggatttag
    33241 ctacagctct agcttttaat acacaggagc gtatgttatt gatagttcaa aataatggag
    33301 ctatagaaaa tatacaagat gatgctatgg ttgaaattcc atgtatagtt ggaaaagacg
    33361 ggtatgaacc attatcaata gggaaaatac caacatttca aaaaggttta atagaacaac
    33421 aattagcagt tgaaaaatta gtagttgatg cttggattga gaagtcttat aataaattgt
    33481 ggcaagcact tatgctatct aagacagttc ctagtgcaac agttgcaaaa gaattactag
    33541 atgatttaat agaagcaaat aaaaattatt ggccaaaact taattaagaa taatatattt
    33601 ttaaagttcc tacagtttag tacatagcct agttaatgta tttgtaggga ctttatttat
    33661 ttacaaatga gatacaagtg gcaaacatag tgtattttgc agttaggaaa gtaggtttag
    33721 ttacttgtta gttggattaa atttaggtac aataatttac tatcaatcaa tagaaataac
    33781 cagagaagtt gcataataaa aaatttacac ttttaacagg atttatgtag gtgtttatag
    33841 aaataaataa atatgtacat aaatacataa ttatttttaa gtctaaaata caagaatgga
    33901 gagatggcta tgaagtggag agattatgct gatgatgtaa atttatttga ggattttgat
    33961 aaaactatca agcctctaac tgacgaacaa agaagaaaaa atataaacac tttaattgca
    34021 tatttttcaa aagaagttcc ttctaaaata tacaatttat caaatgatga aattaagcct
    34081 agagatatac ttagaggact tttgaatgta tatccaccaa aagaaattgc tccagagata
    34141 cttaatatgt tacataattt attgttgatt gaatgtgaag aaagagagtt agtagatgta
    34201 aatgatatag aagaagtaga ggaagggatt gccatatgga gaggtaatat aacgaattta
    34261 agagcagatg caatagtaaa tgctgcaaat aacaagttat tagggtgttt acaaccatta
    34321 catctatgcg tggataatga gatacattca tgcgcaggtc ctagacttag agaagattgt
    34381 gacaaaataa taaaaaaaca agggcattta gaatatactg gagatgctaa gataactaga
    34441 ggttattgtt tacctgcaaa atttgtagta catactgttg gacctattgt atctggtggt
    34501 cagccaagta aagagcaaga aaagcaactg cttcattgtt ataaatcatg tttaaataca
    34561 ataaaagaaa ttgatgaaat aaaaaatatt gttttttgtg gaatttctac aggtgtattt
    34621 ggttatccaa aaaaagaggc agctaatctt gctgtaagta gagttagact atggctaaaa
    34681 gaaaatcctg aaaaaaattt aaaagttgta tttaatgtgt ttactgaaga agaagaagag
    34741 aaatatagaa gaatatttaa ataatattat gtaacagaat cctaaaaata cttattatat
    34801 aatacaaatt cattgtataa gataataatt tacaagtagt aaaaattaat aaaatataat
    34861 ttgtattttt aataaataag taggtataat agaaattata agaatagtgt aatcaattat
    34921 gctattactt agaaaatata atataggaaa attgtatggt aaataaatat gaaaaatagt
    34981 tttaaaaatt gtattagttt aagaaataaa tttataatta tatcaattgt gtttctatta
    35041 atcctattaa ttgtatctta tatatttggc aaattagtgt ataatagttc agttggttta
    35101 gagcagttaa taaaaaacga agatatagta aaaatatttt ctgagagaaa agataaacca
    35161 ctggagaaat taaaaaacta caagacatct gaattaatga tacaaagttc taataattac
    35221 aagcttgaaa gtctttttat aacttcaaat ataaagacaa gagatactat gatacttgtt
    35281 catggaattg gaagtagtta ctatgaaatg ttaaaggtag catatagata tttagataaa
    35341 ggttataatg tattagttta taatcaaaga aatactggta atagtggtgg agataattac
    35401 acatttggtc tttatgaaag gtatgattta gatagtctag ttaaatttgt aaaaaataaa
    35461 tttcctgaag gaagattagg agtgcatgga ttttccatgg gggcagggac agctgctatg
    35521 cattcagaga taaattcaaa agatgataag gtcgattttt atatactgga ttctccttat
    35581 tctgaaatga aagatgcaat tagaatggga gtactagaaa aacgaatacc agatatatta
    35641 atcaattatg tagtgacttg tggagattta tataataagt ttaaatctgg attttggtac
    35701 agtgatgtaa aaccatatga gtcagttgag aaatctaatg tacccatact gtttatacat
    35761 ggaacaaaag atactgtttg taattatcaa aatagcaaga aaatgtatga tttagttaaa
    35821 catgataaaa aagacctatg gcttatagaa ggtattggtc atgttgatgg atttgaacat
    35881 gatagtacag tatacttcaa taaaatattt aaatttatag atagtaatgt tttaagtgat
    35941 aaaagttaat tgaatattag tagcttattt atttttgtat ttattaaaaa atagataggc
    36001 tatttttatg tttaaatata agttttgttt agtaatgtga attaatttct agtaaaaatg
    36061 aaaataacta ccaattaaat tcatttatac ctcaaataaa agagttagta ttagattaca
    36121 atcaaatcta aagaaaagag aggtagtata tatgaaaaac aaaaataata aagataaaaa
    36181 catgttgaat attaaagtat tgagagttct aggagtgcta ttaatacttt ttgattatac
    36241 gggtgatact ttgaattttt ataatttctt cgcaaagcca ttagtatcgg gtaatttata
    36301 taagacaaca ggtactgact gcattgtatt tttattattt attgtagagt gtgtatacct
    36361 agtgggaaga tatatattaa aagttaagat aaattggagt gagattattg aaaatgagaa
    36421 aggtaatttt ataaataact tgttggttga aaaaatacaa gaggaaagca acattgtata
    36481 taatttatta aactcatatg tgttgccaca tatatataag aaagcaaggg gattgatgtt
    36541 taaaacatat catatgttaa tctacattaa agaaaaatac tattatgtaa atatatatta
    36601 atataatatg attgcacaat taattatatt atattttgta ttggatgata aaatgaaaga
    36661 cataaatttt ttgagacact aaaatcgcaa tttatttcgt gtttaagtta taaattttgt
    36721 gaaaaattat aaatcttata aaatagattg cgattttaat cttaaacttt tgctatagtt
    36781 tttccagtat cagttaaatc atatccgcca agaccttgaa gttcagcttt aaactcagga
    36841 gagtttatta tgttaattat agtttggtaa actggatttt gtaagtcttc ttttttaata
    36901 actaaatcat agcgctcttt gtgaagagga ataaagtcga tattatttac ttgtaaagca
    36961 gctttttcat ttcccaaacc aacatctgct attccacgag aaacagtact tgcaactcca
    37021 agatgtgata attcttcatt atcataacct tttatatgtt taccattaaa gtttagaagt
    37081 ctaagtttac catctagtaa aaccctagtt ccagaacctt tttccctatt tacaatggtt
    37141 atacttggtt ttgttaaatc ctcccaggtt gttatctctt taggatttcc tttagcaaca
    37201 tagaaacctt gcattctata agctagattt ataagtacac atggtattcc tggcaatagc
    37261 tttttaacaa aagtgctatt atattcatcc gtttcgaaat cccatagatg ggatgatgct
    37321 atagaaactc tgccattata taactcatac aaagcattat aactacctat ataagagcga
    37381 aatgtactta ttccatccaa ttttttttca acagacctag ctaaaatgtc taatattaca
    37441 tcttgaccag aaataataat tttgaaatta ttatttatat ctatattttt attgttgaaa
    37501 tgtgtatcct ttactttacc agttttttgg ctgttgatat attcttcaac atctttagta
    37561 tcaactctta tttttttccc tactttataa gaaggaagtt cacctctttt gattaattca
    37621 tatacagtat tttttgttat ctttaataat tcagctacct cgacaggtgt caaagatgat
    37681 aatgagtcca taatccacct ccatataagg ttattttaac attaaaaaaa ggaaaatgta
    37741 gcaaaatatt taaaagtagt gtaaataaat tacttgaaaa tttattatca tgtatatata
    37801 attaaactat atataacaaa acatgataaa acataataaa acatgttaaa atgtgtatca
    37861 aatttacaaa aagggagatt gttatgaaaa aaatattggg aatattagga ttagttgcat
    37921 gtttaacttt ggggacagtt gggtgcaata gtaatgaaga caaaaaagat aatggaaaac
    37981 aagaaaaaac aactaaaagt agtgattcag tagagttaaa tatatcggct gctgcaagtt
    38041 taaaagaagc aatggcaaag attgaagaag aatataaaaa agttgattca aatgtaaaac
    38101 ttacagttaa ctatggagca tcaggctcat tacaacaaca aatagaacaa ggagcacctt
    38161 gtgatttatt tatttcagca ggtcaaaaac aaatgaaagc tttggatgaa gagaagttat
    38221 tagtttctga tactatgaaa gatttagtaa aaaatgattt agttcttatt tcttctgctg
    38281 acagtagtgt atcaggtatg aaagatttga ctacagataa agtgaagaaa attgctgttg
    38341 gtgaagctga aagtgttcca gctggtaagt atgcagatga ggtgttaact aatttaaatt
    38401 taaaagataa gttaaaagat aagctagtat ttgcaaaaga tgtaaaagaa gttttagcat
    38461 gggtgcaatc aggaaatgct gatgttggat tcgtttactt cagtgatact gtcaataatg
    38521 ataagataaa agtagttgaa aaaacagacg aaaaaactca ttcgcctatt acatatccag
    38581 tttcagtaat taaagctagt aaaaacgtag atgctgctaa gaaatttgaa gaatttttat
    38641 taagtgagtc aggacaaaag atttttgaag aatttggata taaaaaagta gaataaactt
    38701 agaaataaat tatgtgtaat taagtataaa ttatatataa ataaatgaaa attgaaaata
    38761 attgtgtatt ataataaaat ggaaatagtg aaattaatag agttattagt ttcactattt
    38821 ccatttaaaa ttaaggaggg aaagatggga acagattggt cacccttatg gatatcactt
    38881 aagacatcta ttttgtcaac aataataact tttgtaatag gaatatttgt gtcatatatt
    38941 atggctaact atagaggtaa gtggaaaggt cttatagatg gactatttac acttccacta
    39001 attttgccac caacagttgt aggtttcttt ttattattac tatgtgggaa gaatggattt
    39061 ataggaaagt ttttattaaa tttcgatagg actttaatat ttagttggac agcaactgta
    39121 atttcagcag tagtggtgtc ttttcctatg atgtatagaa cttcaaggtc agcttttgaa
    39181 cagattgata ataatatgtt gtcagctgct agaacattag gtcttaatga atggaaagta
    39241 ttttttaaga ttgcagttcc attagcatgg cctggaataa ttggtggatt ggtgctgtcg
    39301 tttgccagag ctcttggaga atttggtgct acacttatga tagctggaaa tatacctgga
    39361 aaaactcaaa ctatgccaat agctatattt tttgctgttg aaggcggaga tatgaataaa
    39421 gctatgatgt gggttatgat tattgtagca atatctatag caatgatttt attactaaat
    39481 tattggtcgg aatttcagca gaaaattata ggaaagaggt gtggatagtt gagcttatac
    39541 gtggatattg aaaaagattt atcctctttt aaactaaagg tagaaattaa acaagaaaaa
    39601 ggtactctgg ggtttttggg agaatctggt tctggaaaaa gtatgacatt aaaatgtata
    39661 gcagggcttg agaaaccaac aagaggtaaa attgtattaa atgatagagt tctatttgat
    39721 tctgaaaaga agataaattt atcaacacaa gatagaaagg taggattttt atttcaaaac
    39781 tatgctttgt ttccacatat gactgtaagt caaaatatag aacttgggct tttaaaacta
    39841 agtaagagtg aaaaaaagga aatagtagca aggtacttag atatacttaa attaaatggg
    39901 tttgagggga gatacccttg gcaattatct ggtggtcaac aacaaagggt agcactagct
    39961 agagcattgg caacatctcc agatatatta cttttagatg aacctttctc agcattggac
    40021 catcatttaa gaagtaatat ggaaaaagaa cttatgaata tgttaaaaga ttacaaggga
    40081 gatatactat ttgttactca tgatattgaa gaagcttacc gagtttgtga tgatattata
    40141 gtgtacaaca aaggagaagg tcttccaaag agacctaaaa aagagctctt tgaatctcca
    40201 aagtacctta tagaagctaa gataacaggg tgtaaaaata tatccaaatt aaatagactg
    40261 gataaaaata ctatttatgc tacagactgg ggatgcgagt tgacattaaa tagagaaata
    40321 ggtgataata tagaatatgt gggaatcagg gagcatcata ttaaggtctt ggattctaat
    40381 gaagatttaa atgaaaagtt atgtttcgaa ttgattaaca ttgtagaaaa tccttttaca
    40441 tatactatat atgttagaaa aactgattta tctaacgaat gtgtacctat tcaaatagaa
    40501 ttggaaaaaa gtaaaatgag atttaagaaa ggtgacagaa tatatttaga ttttccacag
    40561 gaatatttat tttgctttag atacaattat aacaaaaagg agtaaactat atgactgaaa
    40621 aagaaaaaat gttgtctggg aaaggatact atgcaaatga tgaattgtta gtaaaagaaa
    40681 gagagtattg taaaaaatta actagattgt ttaataatac tttagaggat gaatatgaaa
    40741 aaagagaaga tattttaaga caactatttg gttcagttgg caagcaaata aatgtagaac
    40801 aaaatattag gtgtgattat ggatataaca tacatgtagg cgaaaatttc tttgcaaatt
    40861 atgattgtat ttttttagat gtatgtaaaa ttgaaatagg tgacaatgta atgttagctc
    40921 caaacgttca aatctataca gcctatcatc caattgatgc acaacttaga aattcaggca
    40981 tagagtatgg ttctcctgta aaaataggtg ataatgtatg gataggtggg ggagtaatca
    41041 taacaccagg aattactata ggtgataatg tagtaatagg tgcaggaagt gtagtcacaa
    41101 aagatatacc accaaatact gtagctgttg gaaatccatg tagagtaata aagaaaatag
    41161 aagaataaat atgattgtat aaagtttata caagtaaaat ttatacaatc aaattaagag
    41221 catttacata taatctttaa atagataata cattaaaata aaaatatatg cctgtttcta
    41281 taaattaatt tatagaaaca ggcatattaa tcgtatatag aaaatatctt tctattcttg
    41341 tttagtcttt acataagaaa tattttcatt agaaggtttt tccataccta aagcttttaa
    41401 tatatcttca ttaacaatga tatctgtatc atctgatgta gccacaggca tatcagatac
    41461 tgattcacca tttaaaattt taactgccat ttcacctgtt ttgtaaccta gtttttcgta
    41521 gttgatacct tgacaagcaa gagcaccttt ttcaactggc ccagattcag ctgctataac
    41581 aggtatttta ttctcagtag ctattttaga gactataggc atagaagaag ccactaagtt
    41641 atctgtagga acatataata cgtctatttt gccaactaaa ctagaaatac cttggttaac
    41701 ttcatttgaa gtagttatac ctttttcaac aactttaaat ccatttttag atgcatattc
    41761 ttttaaagca tcaacttgga ctttggaatt tacttcacta gtattgtaca taactcctat
    41821 agtttttgcc ttaggagcaa atattttaag aagttctaaa cctttgtcta cagaaacaaa
    41881 atctgatgtt ccagatacat ttgtgcctgg cttttcaaga gttttaacta atccagcagc
    41941 aacagggtca ctcacagcag taataagtat aggtatatct ttagtagcat taaatgctgc
    42001 ctgagctgat ggagtagata tagcaaatat taagtccttc ttatcagatg caaatttact
    42061 cgcaatactt tgtgtagtag gcatgtcatt ttgagcattt tggaagtcta tatcaatatt
    42121 ttctccatct ttaaatccgt tcttttctaa tgcttttaca aaacctgtcc tagtggcatc
    42181 taatgctgga tgttcaacca gttgagttat accaattttt ttaacttctt tacctttaga
    42241 attactttct tgttttgaat taccagagtc tcctccttgt gaacaccctg taagcataga
    42301 tatactaaga gcaccagcta aaatcaaact tgccaatctt tttttattaa tcatttttat
    42361 atccccctca aaaatttatt gttatatatg ttcatatatt catatgaatt attacaaagt
    42421 aaccataatt attgtaattt taactttatt ttaaagccga gtttaataca atagatatat
    42481 tattttaaac tgcatattca aatttttcta agatttcttc aactgttaat tgttcttttt
    42541 ctttaccttt tacatctaat acaacttcac ccttatgaag cattatcaat ctatcaccat
    42601 aatgaagagc gtgttttaag ttgtgagtaa ccataagtgt agttatattt ttttccctta
    42661 caatcttatt agtcaattca attacttcat ttgatgtttt tgggtcaagt gctgctgtat
    42721 gttcatcaag taataaaact gatggatttg ttaagcacga cataattagt gataatgatt
    42781 gtctttgacc tcctgataaa aattttactt gaacatcaag gtattttttt aaatcaagag
    42841 ataccccttc caataaattt tctaagaaat ctcttttgta acgaagacat tttcttagat
    42901 ttaatagttt tcctttgtta agagctaaag acaagttttc tctaacagtc atagaagggc
    42961 aagttccaag acttgggtct tgaaaaactc tacttactat ttgagttctt ctgtgttcag
    43021 ctaaattaga caagttatgt ttatttaata atacttgacc agaagattct tttataagcc
    43081 c
//
LOCUS       DAGSWI010000018        42595 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.018, whole genome shotgun sequence.
ACCESSION   DAGSWI010000018 DAGSWI010000000
VERSION     DAGSWI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42595
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.018"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            39..881
                     /locus_tag="MJF80_000889"
     CDS             39..881
                     /locus_tag="MJF80_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBZ0279562.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            896..1360
                     /locus_tag="MJF80_000890"
     CDS             896..1360
                     /locus_tag="MJF80_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279563.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1347..2519
                     /locus_tag="MJF80_000891"
     CDS             1347..2519
                     /locus_tag="MJF80_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBZ0279564.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            2563..3498
                     /locus_tag="MJF80_000892"
     CDS             2563..3498
                     /locus_tag="MJF80_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBZ0279565.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKFPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(3772..6453)
                     /locus_tag="MJF80_000893"
     CDS             complement(3772..6453)
                     /locus_tag="MJF80_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBZ0279566.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGGSTTP
                     PDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            6667..7365
                     /gene="yunB"
                     /locus_tag="MJF80_000894"
     CDS             6667..7365
                     /gene="yunB"
                     /locus_tag="MJF80_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBZ0279567.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(7577..8584)
                     /locus_tag="MJF80_000895"
     CDS             complement(7577..8584)
                     /locus_tag="MJF80_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBZ0279568.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            8812..9696
                     /locus_tag="MJF80_000896"
     CDS             8812..9696
                     /locus_tag="MJF80_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBZ0279569.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            9898..11448
                     /locus_tag="MJF80_000897"
     CDS             9898..11448
                     /locus_tag="MJF80_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBZ0279570.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            11477..12124
                     /locus_tag="MJF80_000898"
     CDS             11477..12124
                     /locus_tag="MJF80_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBZ0279571.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(12266..12526)
                     /locus_tag="MJF80_000899"
     CDS             complement(12266..12526)
                     /locus_tag="MJF80_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBZ0279572.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFRYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            12743..14026
                     /gene="hflX"
                     /locus_tag="MJF80_000900"
     CDS             12743..14026
                     /gene="hflX"
                     /locus_tag="MJF80_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBZ0279573.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYANLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDADLDEEDFNIYREYILEK"
     gene            14034..14711
                     /locus_tag="MJF80_000901"
     CDS             14034..14711
                     /locus_tag="MJF80_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBZ0279574.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            14797..15240
                     /locus_tag="MJF80_000902"
     CDS             14797..15240
                     /locus_tag="MJF80_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBZ0279575.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            15545..16189
                     /locus_tag="MJF80_000903"
     CDS             15545..16189
                     /locus_tag="MJF80_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBZ0279576.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(16275..16652)
                     /locus_tag="MJF80_000904"
     CDS             complement(16275..16652)
                     /locus_tag="MJF80_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBZ0279577.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(16652..17038)
                     /locus_tag="MJF80_000905"
     CDS             complement(16652..17038)
                     /locus_tag="MJF80_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBZ0279578.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            17217..17972
                     /gene="nadE"
                     /locus_tag="MJF80_000906"
     CDS             17217..17972
                     /gene="nadE"
                     /locus_tag="MJF80_000906"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBZ0279579.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            18055..18774
                     /locus_tag="MJF80_000907"
     CDS             18055..18774
                     /locus_tag="MJF80_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBZ0279580.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(18941..19945)
                     /locus_tag="MJF80_000908"
     CDS             complement(18941..19945)
                     /locus_tag="MJF80_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBZ0279581.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            20211..21107
                     /locus_tag="MJF80_000909"
     CDS             20211..21107
                     /locus_tag="MJF80_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HBZ0279582.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            21245..22507
                     /locus_tag="MJF80_000910"
     CDS             21245..22507
                     /locus_tag="MJF80_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279583.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            22583..23776
                     /locus_tag="MJF80_000911"
     CDS             22583..23776
                     /locus_tag="MJF80_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBZ0279584.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            24405..25199
                     /locus_tag="MJF80_000912"
     CDS             24405..25199
                     /locus_tag="MJF80_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBZ0279585.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            25294..26634
                     /locus_tag="MJF80_000913"
     CDS             25294..26634
                     /locus_tag="MJF80_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBZ0279586.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNAKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            26771..28003
                     /locus_tag="MJF80_000914"
     CDS             26771..28003
                     /locus_tag="MJF80_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBZ0279587.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELISILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            28043..29185
                     /locus_tag="MJF80_000915"
     CDS             28043..29185
                     /locus_tag="MJF80_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBZ0279588.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            29196..29990
                     /locus_tag="MJF80_000916"
     CDS             29196..29990
                     /locus_tag="MJF80_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBZ0279589.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            30001..31023
                     /locus_tag="MJF80_000917"
     CDS             30001..31023
                     /locus_tag="MJF80_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBZ0279590.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            31122..32498
                     /locus_tag="MJF80_000918"
     CDS             31122..32498
                     /locus_tag="MJF80_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBZ0279591.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(32830..33174)
                     /locus_tag="MJF80_000919"
     CDS             complement(32830..33174)
                     /locus_tag="MJF80_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279592.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            33662..34672
                     /locus_tag="MJF80_000920"
     CDS             33662..34672
                     /locus_tag="MJF80_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBZ0279593.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAIEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            34808..35296
                     /locus_tag="MJF80_000921"
     CDS             34808..35296
                     /locus_tag="MJF80_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBZ0279594.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            35472..37400
                     /locus_tag="MJF80_000922"
     CDS             35472..37400
                     /locus_tag="MJF80_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBZ0279595.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            37577..37987
                     /locus_tag="MJF80_000923"
     CDS             37577..37987
                     /locus_tag="MJF80_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBZ0279596.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            38009..38311
                     /locus_tag="MJF80_000924"
     CDS             38009..38311
                     /locus_tag="MJF80_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBZ0279597.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(38528..39220)
                     /locus_tag="MJF80_000925"
     CDS             complement(38528..39220)
                     /locus_tag="MJF80_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBZ0279598.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMMAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(39246..40433)
                     /locus_tag="MJF80_000926"
     CDS             complement(39246..40433)
                     /locus_tag="MJF80_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0279599.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQRATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            40769..42568
                     /locus_tag="MJF80_000927"
     CDS             40769..42568
                     /locus_tag="MJF80_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBZ0279600.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGSA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFD"
BASE COUNT        15871 a         5090 c         7749 g        13885 t
ORIGIN      
        1 ttataagaaa attataaaaa ttacaaggag gtatagaaat gaaagaaaaa gcagggatta
       61 gaaatgccat aatagcagcc tttataatca caatattggc agaattaatt ggacctattt
      121 catttaaagt tatgggaatt aatgtaactt tacttagtat actatgggct atattcatag
      181 gaatggctgt ttctcctcat ttattaggga gagttatacc agccttaaaa aagtttatag
      241 gggataatga aataaatgta tctccatatt tattatcttt gacactatat ccattaggga
      301 taatgtttgg aattaatgca gggcctaaag ttggtatatt acttcaagca ggaccagcac
      361 ttttattaca agaatttggt catatgggta caatgttgat agcattacct gttggagtag
      421 cattaggtct tggaagaagt gcattaggtg gtactttttc attatgtcgt gacacagcac
      481 taggaattat tggagataag tatggattaa actcaccaga aggtatagga acactaggaa
      541 cttacataag tggtagtata tttggaacat tattattttc atttttagca ccagtgggtg
      601 tacttttagg atttcatcca tatgctttag caatggcatc aggaatgggt agtggttcaa
      661 tgatgggagc tgcaacagca tcattaatga atacagttcc acaaatgtca gagcagattt
      721 tagcatattc agctactagt ggattgatta cagctgttac tggaatatat atagaattat
      781 tcttatcttt accagtagcc aactactatt atagcaaagt agctccagtt attgaaagat
      841 ttagaggaag aaaaacaaga agaacaagta ataaattgta aaggaggata tatatatgga
      901 aaaaactttt aagactatta tggagcagat aaaaatagta ttgtttatat atattattat
      961 ttgctttgga caaatgatag cacttaagac tcaggctcaa gatttgttga ttggaagtgt
     1021 atctggattt ttgttggtga taattgctat tataataaag aattttgtta aaaaacctaa
     1081 tttaccaggt tttgcatggg ctacattagt tgcatttgtt ttatcgcttc caatatctcc
     1141 agtgggggat atcatagcaa caaatgtagg gaaaatcaat tttatgtcta cagtaacacc
     1201 attattagct tttgcaggaa tctctgtagg taatcaatta gatatattaa agactttgag
     1261 ttggaagtta gtagttatat catttatagt tatgacatct acttactttg gttctgcttt
     1321 tatatctcaa acagttttaa gattaaatgg aatgctttaa ggaggtagta aatatgaatg
     1381 aattaaaagc aaaaattaaa gaggaaataa agttattatc tgaagagaaa aagacatcat
     1441 ttgaaaaagt ttctgactat ttattttcaa atccagaatt agcatttgag gagtataagt
     1501 cacaaaaagc attatgtgat ttgttagaag aaaatgaatt taatgtaact aaaggtgtag
     1561 gaggattaga aacatctttt gaagctgtgt attcaaatgg aactaatggg aaaacagttg
     1621 cttttttagc tgaatatgat gcacttccag gaatgggaca tgcatgtggg cacaatatca
     1681 tagggacaag ttctgttggt gcaggtatca tattaaaaga aatcatgaaa aaacataata
     1741 tagaagggac tgtaaaggtt tttggaacac ctgcagaaga aagagtaggt ggaaaaataa
     1801 ctatgattaa agaaggtgta tttaataatg tagatgctgc attaatttta catccaagtg
     1861 atgcttcaat gcctgatgat atatcatttg ctcaagttaa tttaaaattt gattttactg
     1921 gtaaggcatc acatgctgct gcttttcctt gggaagggaa aagtgcctta agtggagtaa
     1981 tagcactatt taacagtgta aatagcatga gattacatct taaagattat gcaagagtac
     2041 atggaattat aactgatgga ggtagcatac ataatataat tcctgaaaaa tctacagcta
     2101 tatttaatgt aagagcctta agtatagaat atttaaatga gatttgtgaa atgcttaaaa
     2161 attgtgctaa aggagctgca atttctactg gaactaatgt agaaatagtt caattagatg
     2221 aaatatacaa agagattaag aatgattctg aattagtgaa tatagttaga caaaattttg
     2281 aagttttggg tgaagactat gttgaaagag atttaagtca aggtataggt tcaactgata
     2341 caggaaattt aactcatgag atacctgcaa tacaagcata tataaaatta aaagaaaata
     2401 ctgcaactca tacagatgaa tttgctgttg cagctggtgg tgaagaaggc agagtagcac
     2461 ttataaaagc aattaaggtg ttagcaatgt gtggagtaga tattttgtat agtaaataat
     2521 atataatttt aaaatatatt aagaaaggaa atcggattag acatgatatt aaaacaagta
     2581 atagaggttt atgacatttt agataaagca aatgctaatg gggaagaagt aaaaacttac
     2641 ttacaaggtt atggtgaagt agatgtaact gtaaaagaat tgtcttcaag taaaggttca
     2701 acagatttag taaagcttac aattcctgga aaaaatggaa aattaaaagg tggagatgct
     2761 cctactcttg gtattttagg tagactagga gggattggag caagaccaga agttataggt
     2821 tttgtctcag atggtgatgg agcattggta gcaattgcag tggctgctaa acttttggat
     2881 atgcaacgaa aaggggatat attagatggt gatgttgtaa tttcaactca tatttgccca
     2941 gatgctccaa ctaaagaaca ctatcctact ccttttatgg attctccaat tgatatgatg
     3001 actatgaatg aaaatgaagt tgatagtagt tgtgatgcaa tactttctat agatactact
     3061 aaaggaaata gagtaatcaa tactagagga tttgcaatat cgccaactgt taaagaaggc
     3121 tacatattaa aaactagtga taacctttta gatattatgc agactgtaac agggaaattt
     3181 ccatttgtat tccctttgtc tattcaagat ataacaccat atggaaataa tctgtatcac
     3241 ttaaatagta ttttacaacc agcagtagct acagatgcac caatagtagg agtagcaatt
     3301 acaacagaga tgcctgttgc tggatgtgct acaggtgcaa ctaattttag tgatttagat
     3361 tcagctggga gatttgcaat tgaagttgcc aagctatttg gtagaaataa gtgtgatttc
     3421 tatgataaag aagagtggga aatgcttatt aagagatatg gcaaattaaa tagatttcaa
     3481 acatttggta tacagtaatt agaaaaatat taagtttata taatttgtga tataactatt
     3541 taagaatata taaacaatta ggaataaaag taagaataaa gcacttagtt aaaaacttta
     3601 aaaataaaat atttatttgt ttatataaaa aggaggattc tggttattgg gattctcttt
     3661 tttatgaata caaataatat attgtaatta taaaaaaagg agcgtttcaa agtgattttt
     3721 ttaatcacct tgagacaact ccttatattt ttatttaaat tctatttttt attaagttgc
     3781 tggagttcca ccaccatcag gaggagtagt agagccacca ccattatttc caccaccatc
     3841 aggaggagta gtagagccac caccattgtt tccgccatta tcaggaggag tagtagagcc
     3901 accaccatta tcaggaggag tagtagagcc accaccattg tttccgccat tatcaggagg
     3961 tgtagaaccg ccaccattat cacctgtgtt attattgtta ttattatttt cttctggttg
     4021 gttattgtct ggtttttttg tatcttcaga tgatggttga gaacttactt tagtaggagc
     4081 agtaccttca ataaatgctg ctttagagcc agaagattgc tttcctccat cctctaaatt
     4141 aatttttgta aaatatacat ttttaggaac ttcaaattca gtaaccgtta aattagcatg
     4201 tattttttcc ataatttttt cccatagttt tgcagtacta gtacttccat gtggtacttc
     4261 tctacgtttt atagtgttac cagaatcatc ttttctacca gcatcatctc caatataagt
     4321 tgcaccaaca taataaggag tgtaaccgac aaaccaagca tcatatgcac tatttgtagt
     4381 acctgtttta cctgctacag gcattccctt tggaagtgta gcaacaccac cagtaccttc
     4441 agtaattacg ctttctaaca tatctgtaac aacataagct acttcagggt ctacaacttt
     4501 atgttcttca ggagcatttt taactaataa ttgaccatca aaggtagtta tagtagtaaa
     4561 aatagaaggt tctacataaa taccgtgatt tgccaatgta ccataagcag cagccatttg
     4621 aagtggagta ataccattcg ccataccacc tagtgtcaat gatgggaatt ttctatcact
     4681 tgcttcagat gaacctgagt gattattctt taaagttgtt ataccaaagt tctttaagta
     4741 atccatcatt atatctacac attcatcata agagtctcca agcatttcag cagttttgac
     4801 agctatcgtg tttgaagatt tagcaagtgc tttacgtaaa ctcatagaac catgaccagc
     4861 agtagtagtt cttggattcc atttattatt ttcttcaaac ttatatcctc ctcgagaatc
     4921 acttagagca gttgcttgtg ttatttttag agtatcaata gctggcgtat atattgctaa
     4981 aggttttata gaagaacctg gctgatgagg gttagtagca cgatttaaag tttttctacc
     5041 acttatattt cttcctccag ctagtgctct tatttcacca tttttataat ctaagattac
     5101 cattgcagct tgcggttggc taactccatc aggaccgacc atatgtcctg ggaaattgct
     5161 atttctatca aattcttctt ctaaagcatc ttgcatttta gggtctatag ttgtgtttac
     5221 aattagacca ccattgtaga ataagttttg agcttcatca tttgtatgtc cttgttccat
     5281 taaagcattt ataacttcac tttcgataag gtcttctaca gatgaagaaa ctttatctga
     5341 tgattgaggc aattttattt ctattttagc tttgattgct tcatcatatt cactttgttt
     5401 tatatatttc aattctaaca ttttctttaa aacaatttct tgtctttttt tagcttcagg
     5461 attacttaca gcctttctaa caactaatgt acctgctttt aattgtctat aagtatcatt
     5521 agatataagc tcccaagatt ttattttttc aatcatatca aagtccactt gagtagggtc
     5581 atctaaatca tcatcagttg tgttaacaaa aaataataat ttattctcta aatcctcctt
     5641 agtttcattt ccctctaact tagaagtttt ataggctgaa aatcttgatg gatttttagt
     5701 tgaaccagca agaagtgctg actctgctag agtaaggtca gctgcagact tatcaaaata
     5761 accacgtgca ccagcctcag cacctgctag acctctacca acgaagaagt tatttaaata
     5821 agtttctagt attttatctt tacttactgt tttactcatc tcatgtgcat agtaaatatc
     5881 ctttatttta cgaggtatag tttgctgttc agttgttaac agcattttgg atacctgcat
     5941 aggaatagta ctaccacctt gcttagtacc tgtaagggtc tttaatactg acctaaataa
     6001 acctttaata tcgacaccct tatgctcata aaaacgctca tcttctattg caattacagc
     6061 atgttgaaga taaggggaaa tcttatctaa tggaacgggt gtctttttat ttacacttgg
     6121 agctgtagat aatgtttttc cattagcgta tttaatagta gttgtttggt tcgtctgttt
     6181 atctaaaaca gcttctgtaa ctggagttac atctcttagt gacgcaaata ctagaccagc
     6241 agtacctgct gtaccaacaa ctagtaaaac taaaaatact atcccaaata ctcttagttt
     6301 tttaaattta tcgctttttt tagatttttt cttgtttttt gttgaacgag tcttgttagc
     6361 tgaagttcta ttaattggtt tatttgtatt accagatgag ctaacttttt ttctcctgat
     6421 tttatttcca tttttatcat tatcgttatt cataagattt taacctcctt acttaatgca
     6481 atattataac acaaatagga gtagttttgt aattaataga aaaaatgaat ttgctataat
     6541 attctgtaca ttgtatatgt ataaacgtaa tatattaaaa agtctatatt tttattttat
     6601 ttatttttga caaatattat aaaaattatg ccaaagctac atataaataa ataggagggg
     6661 ataattttga atagaatagt agatagaaaa acaaaaaatg aatttagaaa aatcatagct
     6721 atttttttag taattctttt cttgtctgta tttataggca gttttatata tatagataaa
     6781 actttaagac cgacaataac agttttagca gagactaaag ctcttgaact agcaaatagg
     6841 tcgataaata aagctgttgc tgagatggta gaaggaaaaa taaattatga agatttaatg
     6901 gatatacaac ttgataataa tggtaaaata actatgatac aagcaaacac tataatgatg
     6961 aatgaaattg catctgcaat agctttagaa atacaagatg aattaaaaaa ggataagaca
     7021 gcaagttctt acatacctat aggaacagca ttaggaagtc ctatattagc taaatacggt
     7081 cctaaacttg aagtatctat tgaacctata ggaacagtat cagtaaactt taaaacggaa
     7141 tttgagtctt caggtataaa tcaaactaga catagaatat atttagaagc tcaaacacaa
     7201 gttaaagtag taataccact tataacttca acaaaacaaa ttaaagctca aatacctatt
     7261 tgtgaaacta taatagtagg tgatgttcca gaaagttatg taaatattcc agaaaaaaat
     7321 ctaggaaatg ttttacctaa cactggaaag aataccaaca agtaatgtaa agttgtatat
     7381 ttttaaataa attcaaaata atagtttata aataaaattt ttggaaataa tattaatata
     7441 attttaataa aaatttatat tttatactat gtaaaatatc taatatattt ggtctgtagg
     7501 ttaaataaaa gtacctttag accaaatata ttattaataa cttaataaca tcttaaaact
     7561 tatttattta tattatttac aacatccatt taataaatac accataacct acagctgggt
     7621 cgttttcaat tgcatgtgac actgctccga ttattttatt atcttgaact attggagtac
     7681 cactcatacc ttgtacaata ccaccagttt gagctaaaag ctttttatct gtaattttaa
     7741 ttttaaaact tttactttct ggatgttttt ggttttctat atctataatt tcaatattat
     7801 attttttaca cttattaaag ttatcttgta aaattatttg agcttttcct aatttaactt
     7861 catctaaaga tgctacttta tattttttta acttggaaat atcaaagtta ttaatttgac
     7921 ctcttattcc aaaatcagta ttttctgtaa agctaccaat aatattttct cgttttgcat
     7981 ttatacatcc aacactacct ttataagatt tttctatatt aaggtgctta gttgtagaaa
     8041 ttgaacctgt tttaataggt atttttcttg cattaccaac acttattgga tgacctacag
     8101 ctccaaactc tgatgtatct ggattgatat acgttaaagt agcaaatcca gaaagtaaat
     8161 tgttaaaact aattttttct aatatgtgtt tggtagtttt cattgttata agttgtccac
     8221 ctctatttaa taaaatagat acttgttttt cttctggtaa agcatttaag gtattggcaa
     8281 agtcactata tccatcaact tcagcattat ttacctttac tatagcatca cccaatctaa
     8341 atggagatgc ttctccagga tttctaacta tgatattttt taattctgcg tcaatttgaa
     8401 gtacgtttcc aattggtatt aaatattcag gttcattttt ttgtaaattt tgtgcataaa
     8461 ttaaattatt tgagaaaaaa tataataaaa ataaaaatgt taaaacaatt gtaaattttg
     8521 acttagctaa gttggttttt ttacttaaag tattattttt aaaatgaaaa tttttaagtt
     8581 gcataaaaca cctctttctt attaaattat tttaataata gaattgaagt tttcttgctg
     8641 cttctatatt atgttgtgca agaatcatgt tttgatgctt ggaaaactag ctcaataaca
     8701 ccttagtttt cataaaaata aataaaataa atagtgtata tgataaaata aatacatatt
     8761 catataaata aaaataaaca ataactgtct agcataagaa aggagcaaaa aatgaaaaaa
     8821 tatagattgt tagttacttg tataattgcc atgggaatca taattatagg aacatcaaat
     8881 ccagttagca atttttacaa gactacaaat ttgattagtt caggaaatat cacaaatgaa
     8941 aaagcagata aagatgaaaa ttcaaacaaa aataataaag ataaaaatga agacaaatct
     9001 aattctaaaa gtaataaaga taagtcatca gaggacaaat cgtcagataa taaaaatgaa
     9061 attcaaaaaa ataaaaaact tttaatttgt atagacccag ggcatcaagg aaaaggagat
     9121 agcaatttag aaccagtagc tccaggttct tcctctaaaa aagcgagagt atcttcagga
     9181 acagagggta ttgctacaaa aaagccagaa tatgttttaa acttagaagc atctcttgtt
     9241 ttaaagagta tattagaatc taaaggttat aatgtaataa tgactagaga gacacatgat
     9301 gtaaatataa gtaattcaga aagagcaata cttgctaatg acaaaaaagc agatatggtt
     9361 gtaagaatac atgcagatag tttaaataat tcttcaaaaa ctggagcatc tatattgatt
     9421 cctgaaaaag atggaaaata tacagctcct atatatgaag aaagtaataa atgtgctgaa
     9481 tttatcaaac agaatatgga acaatcaggt atacaaatca atggaatcgt tcaaagaggt
     9541 gatttgactg gattcaactg gtcaaaagta ccagctgtat tagtagaaat gggatttatg
     9601 agtaactata atgaagacca aatgatgtca aatccagact accaaagaaa aatgatgcag
     9661 tgtatagctg atggattgga tgcgtatttt aaataagata tgaatagaga aaaagttagg
     9721 ttggtattat ttgcctaatt tttatctcta ttttttattg tacataacac aaagaagaag
     9781 tttattttag gttaaattgt cgtaaatata actaaaaata ttgggaatac ttgatttaag
     9841 tttaaaaaga atatattata gtatagaatg tgtaaaattt ttttgagagg tgttgaaatg
     9901 ctagtagtag aaaatgtgag tcatggattt ggtgcgagaa caatattaga aaatgtatcc
     9961 tttagactta gaaaaggtga acatattgca ctagttggtg ctaatggaga aggaaaatcg
    10021 agttttttaa atataataac aaagaagctt atgccagatg ctggaaatat aaaatggtct
    10081 tcaagagcta cagtaggtta cttagaccaa catacggtat taagcaaggg aaaaactata
    10141 agagaagtgc ttagagaggc ttttaagcat atgtttgact tagagcaaga aatgattgct
    10201 atgtatgaca aaatgggaga agctagtgat gatgaaatga gtaagcttct tgaagaaaca
    10261 gcagagatac aaactatact tgaaaatagt ggtttttata tgatagatgc aaagatacaa
    10321 gaagttgcaa atggtcttgg tcttggagaa attggtcttg ataaagatgt aactgactta
    10381 agtggagggc aaagaacaaa agtacttctt actaaacttc tacttgaaaa tccaactatt
    10441 cttatattgg atgagcctac aaactattta gacgaagagc atataacatg gcttactaag
    10501 taccttcaag agtatgagaa tagttttgtc ttagtttctc atgatattga atttataaat
    10561 aatacatgta atgtaatata tcatatggaa aatggagagt taaatagata taaaggaaat
    10621 tatgatgaat ttgtaagact taatgatata aagaaacgtc aagaagaaca agcttatgat
    10681 aaacaagttg aagaaagaaa aagactagag gattttgtag ctagaaataa agctagagta
    10741 gcaactagag gaatggctaa cagtaggcaa aagcaacttg ataagatgga aatattagaa
    10801 agaccaaaag aaaaaataaa acctactttt gcttttaaag atgctagagc agctagtaaa
    10861 ataatttttg aaacagaaaa tttagtttta gggtatgatg aagcattgac aaaaccactt
    10921 aatttccatt tagaaagagg aaaaaagatt gccttaaaag gaatgaatgg tattggaaaa
    10981 tctaccctat taaaaacttt acttggaata ataaaaccat ttgaaggaaa tgttaaatta
    11041 ggtgactatt tggaagttgg atattttgaa caagagagtt caagagaaaa tagtaataca
    11101 cctatggatg aagtttggag tgaatttcca gggcttacaa actttgaagt tagacaggct
    11161 cttgcaaaat gtggacttac aaatgaacat ataacgagtc aaatgagagt attaagtggt
    11221 ggagaagctg ctaaggttag actttgcaaa gtaatgttaa aaaatataaa tttcttagta
    11281 ttagatgagc ctacaaatca tttagatgtt gaggcaaaag atgaattgaa gaaggctatc
    11341 aaagagttta agggaacagt tcttttagta tgtcatgagc cagaatttta ttcagaaatt
    11401 gttgatgatg tatggaatat agaagatttt acaactaaaa tcgtgtagtg atttaactta
    11461 ttgctggggg gatttcatga aaaagtttct aaatgtactt ttttcaattt gtattagtat
    11521 tgttataata gtaggtgtta ttaattttac tgttggattt aaacaacttt attattttga
    11581 cattgattac ttaaatatat cagagttatc tggtttatct aaagatgata taaaattgaa
    11641 ctacgattat ctaattgatt ataacttaaa taagaatgtg tctgagttca aattaccaac
    11701 attaaaatct tctccacaag ggaagataca ttttgaagaa gtcaggaata tatttcaaaa
    11761 tataaataaa ttagctaaat tattattggt agtttcttta gtaggaatta tattgagtgt
    11821 aaaaaataaa aatataaaaa tcttaaaaac tacatcaata acacttataa ttatgccatt
    11881 attattaaca gttccaattt tattaaattt tgaaaagtca tttattattt ttcataaatt
    11941 attatttaga aatgattatt ggatatttaa tcccgattta gacccagtta taaatatatt
    12001 gccagaggaa tttttttttc actctggaat gatgatttta atattaattt tgttagtaag
    12061 tatagtactg tttgtaatgt ataaattatg taaatcttta tcaataaaaa tatttcaatg
    12121 ttaaatagag ggatattttt atttccaaaa taagataata aaaaaggatt agattaaaag
    12181 ttatacaata aaaatagctg tgcaataata tttagactat gaaagtagtt gaaaaatatt
    12241 tatgcacagc tatttataat agaatttact ttttactagt gattttaact aaatcttgaa
    12301 atttactcat aatgtaattg taattatctt ttttatgagg taatatacaa ctactgcaat
    12361 ctttgatacc tttatctgta tacctaaagt ttccaccaca gttttctcct aaagcataca
    12421 aaggacaata gcaaaataga cagttaaact cttctggttt attagtttta tgacaaggga
    12481 aaaattcgca atctttgtga ttaaaaaact tataattttc gcccatattt tcctcctaat
    12541 taaataaaat ttaaattttt tatactatat aagattttgg tgataatata attatactaa
    12601 ataattttat gaatagtcga taatatagta ttattaatca tagtattaat ggtataatta
    12661 gtcaacagaa aaataatgta aaaataatgt aagtgtattt tgtaattaga atatgtttta
    12721 taacaaaaaa tgaggtgatt aagtgaaaga aattcaagaa aaagcacttc ttgtaggtct
    12781 gaatttgact acaatagtga aaaaaaatga tgatatagac actaatgaat ctatggaaga
    12841 attaaaagaa ctaacaaaag ctgctggggc agaagttgta ggtagcttaa ttcaaaataa
    12901 acactctgta gatgcagctt attatatagg aaaaggtaaa gtagaagaaa taagagccta
    12961 tagtgactct ttagatgcta ctttagtaat ttttaatgat gaattatctg gtgctcaaat
    13021 cagaaatata gagaatgttg ttggaagaaa agtaatagat agaactactt taattttaga
    13081 tatatttgct caaagggcac ttagcaaaga aggtaaatta caagttgaat tagcacaatt
    13141 aaaatataga ttgccacgac tttatggaat gggtggtgag atgagtagaa caggtgcagg
    13201 gattggtact agaggtcctg gagaacaaaa actagaaata gataagagac atatacttaa
    13261 taaagcagca gatataagac gtgaacttaa agaagtaaaa aagaatagag agacacagag
    13321 agttaagcgt ttaaagtcaa atataccgat tgtagcactt gttgggtata caaatgcagg
    13381 aaaatctacc ctgttgaacg aattaataaa gacacacaag gattatgaac aagaaaaaga
    13441 agtttttgta aaagatatgc tttttgcaac tttagatgta actttgagaa aggctctttt
    13501 gccgaataaa aaagaatttt tagtagtaga tactgttgga tttgtaagta aacttcccca
    13561 tgatttagta gaagcgttta aggcaacatt ggaagaagta cagtatgcaa atttaatctt
    13621 acatgtaatt gatgcaacaa atactagtta tgaattgcaa aaaagtacga ctgaaggtgt
    13681 attaaaagaa cttggtgtaa atgataaaaa acatatactt gtatataata aagtggacaa
    13741 gctagaattg gatatatacc ctaaaagtca agaagatatt gtgtatattt ctgcaaaaca
    13801 aggcataaat atggataagt tattaaatat gatagaaatt gctttaatgg aaaatactta
    13861 ttcagtaagt ttgatgttac cttatgagag aggtgatata tttagtagaa taaaagataa
    13921 atataatgtg gagaattttg agtatgggga aagtggtata acgctagatg cagatttaga
    13981 tgaagaagat tttaatatct atagagagta catactagaa aaataggttg aatatggata
    14041 taaactggga aaatatagat aatcttgaag attattttat aacctatctt ctttataaag
    14101 aatctaaaac agtatctcaa attagtaaaa ttagaaatat ttcaagtact gaagcaagtg
    14161 agcaacttat acaagcaaag ttaaaaataa aggaaatgca aaaagatgat tttgaggcat
    14221 ctaaggatat tttggataaa tttctagaac tagataaaat taagagactt gattttatgg
    14281 atagcttaga tgacgaaaaa atggtatact ttaagagaaa ggttttcaaa agaatccttg
    14341 tagaaaaaaa tgctgaagat ttaattgttt taatttgggc gacaggtgag ctaaaagatg
    14401 ataggttttt gaaattgctt catcagctca caaaccacag acactcagat attagaagaa
    14461 taacttattc agcaatcaga aagattgaat caccaagtag tagagaagta ctgcaaaaag
    14521 gtctctacga caaaaatgca cagactaggc agtactgtgc aaaagctctt tcaaaattgg
    14581 gagatgaaaa cagtttaaag attttacagc agttgaaaga caagaataag aattttgaaa
    14641 aagaatatgt gcttagagct tatgacgaag ccattaagtc attggaaaat gcaaaaaatt
    14701 tgaaaaagta attttttatt aaagcaattt gtttacttaa tgatgtattt ctaaagttta
    14761 acaaggtaat aaataaatta atgggagggg tttcaaatga tagtaagacg ttgtgcaggt
    14821 ggagtagtgt tttatgctaa taaagtactt attgtaaaaa atgatagggg tgaatggaca
    14881 cttccaaaag gaaagatact aggaggggga cttccatatg agagtgctgt tcaaagagtt
    14941 aaagtagaaa ctggcataga tgctaaaatg atagatgtgg ctggcgatac tatgtatgaa
    15001 tttttctcaa gaagtagaca acaagaagtt tgtaatgcaa taatgtggta tgttatggaa
    15061 gcttgtaaca ctgattgtgt acttgctcct gaatttcaag aaggtggatt ttataaagta
    15121 aaggatgctt tagaaatgtt atcacatcat aaagagcaag ctttagtaga agtatcttat
    15181 aaaaagttta aggaattaaa gaaattagtt tcagagggcg atgatgttcc agcaaattaa
    15241 ttgtgaattt agaggattct gaggaaggga tagccccttc cttttttaat tgaagcagtt
    15301 ttaagcgtaa attatatagt ctctttaaac tatgatttat caatttagag atttagtagt
    15361 ttatatttaa aatcagtaag atatatttaa aataaaaatt aaaaagtata caattttatt
    15421 atataattgt cttatgatgt tgattaaaga agtattaaaa gtccatttat ttttaaatag
    15481 aatgatttta taaatatata tttgatgtgt atattagaaa atgattaaaa taggaggaaa
    15541 tacaatgagc aattacagga cattacatga atttggaacg gatgaaataa cgattgaaaa
    15601 gtctgttttt attggatatg ctaagcctat aaagagtgaa gaagaagcat tagagtttat
    15661 aaatgaaata aagaaaaagc ataaagatgc aactcataat gtgtgggctt atactgtagg
    15721 taaaaatatg aatatacaaa gatatagtga tgatggagaa ccacaaggaa cagctgggat
    15781 acctacatta gaggttataa aaaaagaaga tttgagagat gtagcggttg ttgttactag
    15841 gtattttgga ggaacaaaat taggtgcagg tggtctagtt agagcttata caaaaggtgc
    15901 taaactggga cttgaagcag gtaagataat ttataaggta atgtatcaag aagttaaggt
    15961 taagatagat tatactcaac ttggaaaagt gcaaaatgag ttaatgaatt tggggtattt
    16021 tataaaagat actgtatatg aggataatgt tgaaatagta gtatattcaa gattagaaga
    16081 tgtagagaaa ttatcagaaa aaatgattga tataacaagt ggaacaggaa aaatagtttt
    16141 aggagaggaa ttttatttgt ctgaacaaaa tggagaaatt ttaatataat ctaaaaggtt
    16201 attagaatag ttaatacctc aaatagccaa ttagtatttt taagtgaaaa gccattaaat
    16261 tgatttaatc taaatcaatt taatggcttt ttatcggatt ttgcacagaa tccttttttt
    16321 acttcaaata aaccgactaa aagtaaaaat atcccaaata ttttttttaa tatatctgaa
    16381 gacagataat tagctaatag tgaaccaaat acagctccta aaaccccaaa cacagctata
    16441 ggtataacca atttaaaaat tacattttta ttttttatat gtatagcaag tgctataagt
    16501 gtcataggga tagatgaaag taaatttacg ctttgtgcta ttttagtgtc tatgccagca
    16561 agtaaaacga gtgcaggtat aaggattgtt cctccaccca ttcccattcc tccaataatt
    16621 cctgcaaaaa atccaattag agcaaataac attaaaacac catccttaat gctgcaatta
    16681 tcataattat tccaaaagaa attctaagaa actttccagt aaccttattt agtaatttag
    16741 ctcctataat accaccaaga atacttccaa ctgcaacttt aattgttaag ttaacatcat
    16801 aagtaccttt agatacatat aaaacagaac ttgctgtcgt taagaatatt attatagaaa
    16861 gagcagttgc atgagattta tgttcttcta ccttaacgat gtcatttaat attggaacta
    16921 aaagagtacc tcctccagaa ccaaaaactc catttataaa tcctgtaaaa actccaatta
    16981 tcgtattttt taaattaaaa tcaaagtatt ttttctttaa atcatcagtt tttttcataa
    17041 aaatcacctt ctgaaatagt tttaccaaaa taggcaaatt atatttatat aaaataagat
    17101 taaatataat ctttcatgtt tgtttaattt tgcataaaaa aaaacatatt aaaacaacat
    17161 attaatatat ggtataatat attaaaacaa agtagcaaat ttgagaggag ataacaatgt
    17221 caaatataaa aatacaaata gacaaaactg tagaatggct tataaataag gttaatgagg
    17281 caaatgcaaa aggattaatt gttggtgtat ctggaggaat tgattcggca gtagttgcta
    17341 atttaattaa aaaggcattt ccagaaaact ctatgggagt tataatgagc ataaaaagta
    17401 atcctcaaga tagagaagat gcacttaagg ttatagaggg atgtgatata gaataccttg
    17461 atttagacct tatagaacca caaagtgcta tattagacat ggtagtagga aatttaaaag
    17521 ataaacattt atatagagaa gaatatttaa agatgacaga tgcaaactta agagcaagag
    17581 taagaatgag tacaatttat acaattgcta acaatctagg atatctggtt gttggtacag
    17641 acaatgctgc agaaatacac acaggatatt ttactaaatt tggtgatggt ggagttgata
    17701 ttttacctat tgcaaattta actaaaggag aggtgtatga atgggcaaaa gagcttggtg
    17761 ttcatgaaga tttaataaac aaagctcctt cagcaggtct ttgggaaggt caaacagatg
    17821 aagatgaaat gggaactact tacaatatga tagatgctgt attagaaggt agattggatg
    17881 aagttcctaa aagagaccaa gagataattg aaagacttca tagattaagt gaacacaaga
    17941 gaaaaacgcc tgcacaacct cctaagttct aatttactat ttataagatt tgtgataaaa
    18001 tttacaagtg atgaaaatta gaataattaa ttgtacgtat taaggagtgg aaatatggga
    18061 cgtataggta atataattaa tagaaaaggt aaacaagatg ctcaaagagc aaagatattt
    18121 actaaacatg ctagagcaat agcagttgca gctaaggaag gtggagcaga cccagagtac
    18181 aatgcagcgt taaaaacagc tatagaaaaa gctaaggcag ataatatgcc aaatgataat
    18241 atagatagag ctatcgctaa aggtgctgga gcaggtgctg gagaagacta tgaaactata
    18301 gtttatgaag gatatggacc tggtggagtt gctgttatag tagaaacttt aactgacaac
    18361 aaaaacagaa ctgctggtaa cgtaagatac tattttgaca aaaacggagg aaacttagga
    18421 actagtggat gtgtgtcttt catgtttgat aaaaaaggac aaatattagt tggtttagga
    18481 gatggagttt cagaagaaga attgatggat gttgcattag aagcaggagc tgaagatttc
    18541 ataacagaag aagatggtta tgaaataata actacaccag aagatttttc tagtgttcgt
    18601 gatgagttaa aagctaaagg atatgagttt atatctgctg atgtgaagat gatacctcaa
    18661 actacaactg tattgactga agaatctcat ttaaaaatga tgaataaatt agttgatatg
    18721 cttgaagaag atgatgatgt tcaagatata tatcataact gggaagtaga ataaatagta
    18781 taagcatatt gaaatttata agtattgaat caattttaac atgtgataaa ttttatagaa
    18841 taagaaacac taaaatagac atacgcacct ctaacctata tttgcataat aatatgtaag
    18901 cataggtaag gggtgttttt atatttatag gttaagaaat ttaaagtaaa ttttgagctg
    18961 gactcgattg tctataaata attgatgggt atataacttg ttgctgaata ccatttatac
    19021 gcccagttac tgtatcaata atcatcctaa gactacgttc tgctgtctct tccattttta
    19081 aatctataac tgtcaaagga ggaatacttc ttgccattag tcgtgaaggt ccattacttg
    19141 ctgcaaatat ttgaatatcc tctccaatta caattcccga ttcatgaaat gcattcatca
    19201 taccaagagc taccacatca tgattacaaa aaattacttt ttttagtgaa ttttttgcaa
    19261 gcattttttg agcaatatca tatccactat ctattttatt agaacaataa tatatagaat
    19321 ctgatagatg tattccatgt tctttacatg tttttaaaaa agatttaccc cttaaagtca
    19381 tacatgtaaa ggaagttggg ttgaatacaa cagcaatatc atttttacca ttttgaatag
    19441 catgtaaagc tgctatacga ccagtttcct cattatcaat agatacactt gaaaaaccag
    19501 gtatttcacg atttacaaca actacaggaa ttttaggttt attttttact agatattcta
    19561 tatcatcagc attgatacct gttaaaacaa ttccatcata ataattattt gcaaaaaagt
    19621 attcatcatc acatagatga ccaactttgt aaggtcgtat gatgatattt atgtttaaat
    19681 gttctaaaga aattatgtgg tttattgctt gaagtatatt ggttgatgcc aactcaaatc
    19741 cctcatcctg ccaataaaaa gctattttaa tattattatg tttaatagga gtagagcgtt
    19801 tatatccaag cttatcagct gaatttaata taagctcttg tgtatgtagc ggaatcctgc
    19861 gttcatttcc tttaccactt aagacgtacg agacagttgc tgaagaaaga ccacagtctt
    19921 ttgcaatatc tttgattgtt gccatgataa aatcctcctt aaatagttgc ttaaatttgc
    19981 ttataccaat aatcttaaca tatattcaac taaagaacaa tattctacaa ggtgcatatt
    20041 ttacaggatt agaataaata aattaaaaaa aacttcttac ttatgttgac ataatttagt
    20101 ttataggatt ataatatgct taaataagct tatgattaac ttgaattaag caaagttaag
    20161 caggaacatg caatgctagc ttgcaatgtg actgcaaagg gggaaaaggt atgagtaagg
    20221 taaatatatt tgaagttggt cctagagatg gatttcaaaa cctgaaagag tttttagaaa
    20281 ctgataaaaa gcttaagatt attgatggtc ttattgcttc tgggattaaa caacttgaaa
    20341 ttagttcatt tgtaagccca aaagcaatac ctcagatgaa agatgcaaaa gaagtagcaa
    20401 cttattgtgt agaaaaatat cctgatgtga aactttatgc attagttcca aatttatatg
    20461 gtgctaaagt tgcatgggag tgtggtataa gagatatatc ctatgttata tctttgagtg
    20521 aaactcataa taaaaaaaat ataaatcgta cccatgaaga gtctattgat gagcttaaaa
    20581 aaataatgaa tacttatcca gacatgaata tatgtattgg aatggctact gtatttggat
    20641 gtccttttga aggtttacca aaaattgaag atgccataag atttgctaaa cgcctttggg
    20701 aaaacggtat cagggagata aatctagcag atacaatagg gattgctgac cctaagcaag
    20761 taagagaaac tattagtgca ttagttgaag ctttaccaga atgcagatgg caagtacata
    20821 ttcatgatac tagaaatatg ggaatggtta atactcttgt ggctattgaa tctggaatta
    20881 ctactgttca gtctacactt ggaggattag gaggatgccc atttgctcct ggagcatctg
    20941 gcaatactgc aactgaagat ttagtatata tgcttgaacg tatggggtat gatacaggag
    21001 ttgattttgt taaacttctt gaattatcaa agtatcagaa gagttttgta aatggagtat
    21061 atagtggtca tcatgtaaat ataaagtctg atagttgtaa ttgttagttc tattgattaa
    21121 tcgattaaaa aaatatgttg attaaaaaaa tatgctgatt ggtaaaatag atttattgat
    21181 aaaaaatata aattataaaa tacattatct aataaaacaa ataaaaaaat tgagggggat
    21241 tagtatggat attatagctt tacttttatt tttaggagtt attattattg catttgtacg
    21301 taagaataat gcaggtattt tggctctagc tattggtgtc atagctgtta gaatttttgg
    21361 aatggatgat aaagaacttt atggagctat aagcaactca atgtttacta cacttgttgg
    21421 tataacacta ctctttgcaa tagtgaactc aacaggagca ttaaagcttg ttgcagaaaa
    21481 aattgtagct gcttctggta aacgtgtttg gcttatacct atagcagttt ttatagcagg
    21541 atttgttgtt gctggtgttg gacctggggc aattccagca cttgcaatta tcccagcaat
    21601 ggctgttcct gtagccataa cggttggtta taatcctgta atgcttgcac ttataggtga
    21661 atgtggatta atggcaggtc gtatgactcc tattactcca gaaggtcaaa ttataacaac
    21721 cgttgcagaa ggggttggaa ttgaaaatgt tatgcctact atcttagcct gtcaaactct
    21781 ttctacaatt gtattttcaa tagtattgtt tataattttc aaaggatata aacttaaaaa
    21841 acctataaat gttatagata gtaagcaaat agaaaaattc aatataaagc agataatttc
    21901 tctaataagc atagtagtta tgattgcttt tatcataatt tttgatatga atgttgggct
    21961 tgcagctttt gcagtttcag ctgtacttat ttttttcaat attgctgatg aaggagaatg
    22021 tatcaaatca attccatgga gcacgatagt aatggttctt ggagtaggtg caatgatgac
    22081 aatcgtagat gcagcaggag gtattgactt gatgagtaat gcattatcat cactgatgaa
    22141 tagtaaaaca gctactccaa ttatgagtat ttcagcagga cttatgtcta tggttagttc
    22201 tgctttagca gtagtttatc caactatgat gcctatgtgt gttgatatag ctaaggcagt
    22261 tggaggagta gatccactag cacttattgc tgcagttggt gttggaggtt ctcttgctgg
    22321 tgtaagccca ctttctactg gtggggcatt gattttagca gctatgggtt caagtaagaa
    22381 agactttaat aaggaagaac aaaataaggt ttttgtacag cttttcatta tgagtgcagt
    22441 tggcttattg gttattgcag tagtatcaat attatcattt aatgcaataa cacatttaat
    22501 gaactaatta gaataactga aaataaatca ttaaatttaa cttttaatat taatcaatat
    22561 aatataattt ggaggggaag ttatgaaaaa aggagcatta agcaatataa aggtacttga
    22621 ccttacaaga gttcttgcag gtccatattg tacaatgatg ttggcggatt ttggagctga
    22681 tgttataaaa atagagatgc caagaaaagg tgatgataca agaggttttg gaccttttgt
    22741 aaatggagag agtttatact atgcaaatat aaatagaaat aaaaaaggta tcactctaaa
    22801 ccttaaatca gaagaaggaa aaaatatatt tttagaaatg gtaaaacatg ctgatgttgt
    22861 agtagaaaat tacagaccag gtgtaatgga aaagttaggt ctgggttatg atgagttggc
    22921 taaagttaac aatcaaataa tttatggagc tgtatcagga tttggatgtt atggaccata
    22981 ttcagaacgt ccaggttatg atattattgc acaggcaatg ggtggtctta tgagtataac
    23041 agggcaagaa ggaggagaac caacgagagt tggtaatgct atgggtgatg tactaggtgg
    23101 aatgaattta actattggta tactcacagc actcaacgct agaaaaataa taggaaaagg
    23161 acaaagagta gatgtttccc tcgttgattc tgttgtatcg tctctcgaaa ctggtataca
    23221 gagatacttt gattcaggac aacctgaact tatgggtaat agatatgctt cagcctatcc
    23281 ttatgactct tttaaggcaa gtgatggaag ttttgtaata ggctgtggta atgacaaact
    23341 ttttgaaaag ttatgtaaaa aagttctttt tagagaagac cttttaatag atgagagatt
    23401 taaaacaaat cttaatcgtt gtgaacatta caaggctctt aaaccagaaa tagaaaaatg
    23461 gtctgttaag catacaataa aagaagcagt tgacttaata tcagaggctg gagttcctgc
    23521 tgctcctatc tataatcttg attgtgttac aacagatgaa catatagcaa aagcaagaga
    23581 aatgattgta gatattagtc atcctattat aggaaatatg aaagtcaatg gaaatccagt
    23641 taagttgatg acaacaaaag ctaaaattga tatgccagca ccatctctag gggaaagtaa
    23701 cgaggagatt tataatggtt ggcttggttt tgatagtgat aaaataaaag atttaaaaga
    23761 aaatggagtt atttgattaa aaaatagcta aacacacttg tgactttagt catgggaggt
    23821 tcagttagaa taatatcaaa aaatattaaa tcaatactgt aaataaaata tagaattgtc
    23881 agaagcattt aagatgggaa aagctactta gacattgagt ctttgtggct tttcctattt
    23941 tcattaagaa ttttatatta tcagaataga tatgtactaa actaaagtat taacaattta
    24001 aattaataga aaataaacta cctaattatg ttgaataaag actactttag tgaaaacaaa
    24061 cttatagaga tgctatttaa attattttaa tgggaatatc aaaaatgaga ctgcattctc
    24121 aaaaatgaga ctgcgttttt taagtatttt ctcgaaaatg agacaaaaat attattttat
    24181 gggttttatt gaagaaaaat tcttctgtga attacaattt aatcaatttt aaagttgaaa
    24241 attgttaaaa atttagtgaa atttatgtat atttcgttta ttttattaaa aataacaatt
    24301 ttttactaaa aataaagaac ttcaatggtg attggcatag tacttgctat tttatattaa
    24361 caagaaatgt agaaaaaata acaatgtata atggggggaa tatgatggaa tctgtaatgg
    24421 aaaatttgaa caatcttaag gtggaggtta aggacaaagt ctgtgttatt actataaata
    24481 gacctaaagc tttaaatgct cttaatagtg acactcttag ggaactatca caagttattg
    24541 atgtagtatc agaaaatgag gcaatattag gagttattat tactggagaa ggtaaagttt
    24601 ttgtagccgg tgctgatata cgccagatgc aaaactataa aagtgaggaa ggcagaaaat
    24661 atgcaggtta tgctcaggga atttttgata aaattgaagc tcttgagaaa acagtaatag
    24721 cagcagttaa tggatatgca ttaggtggtg gatgtgaact tgctatgagt tgtgatatta
    24781 gaattgcatc agaaaaggct atatttggac aacctgaagt taatttagga gttattccat
    24841 gttttggtgg aactcaaaga ttatcaagac ttgttggaac tggaatagct aaagagttaa
    24901 tttttacggg gagacaagta aatgcagaag aagctaaatc tattgggctt attaataaag
    24961 tggttccaag tgatttactt ttagaagagt ctatgaagat gatgaaccaa atagttgaaa
    25021 aagcgccaat agcaataaga tatgcaaaag ttgttattaa caaagggata gatatggatt
    25081 taaagaatgc tcttgaacta gagaaagata ttgcaggttt aacttttgct acaagagata
    25141 agcaagaagg aatgaatgca tttatagaaa agagaaagcc agtttttgaa aataagtagc
    25201 tattataact aaaattggaa caatgtagaa tatattcaaa atctaaattt cgtgaaaaaa
    25261 tacatatagt ttaattttaa aaggagttaa tttatgaata cgggagtaat tataagttta
    25321 gtagggatta tcatagctct atctcttcta gttgtaatgg ttatgaaggg aataaatatt
    25381 tttattatag caattgtatg ttcatcatta gttgctataa caggaagcct aaatgtgtat
    25441 gatgcactta aagtggatta tatgacaggg tttgttggat ttcttcaggc aaatttcttt
    25501 attttcctaa caggtacatt aatgggaaaa ataatggaga ttactggtgg agcaaaatct
    25561 attgctaaga tgattgtaag atggattggg aaggataaag cactactttc tattccaata
    25621 gcttgtggaa tattagccta tggtggagta agtgttttcg ttgtaagttt tgcagtgttt
    25681 ccgatagctt tagaaatttt taaagaagct gatttgccac gaagatttat accagcagct
    25741 ttgacttttg gatgctcaac ttttgctatg gttgcaccag gggcaccaca aattcagaat
    25801 attgttccag catcaacatt agggacagat attatggctg gagcagtaaa tggttttata
    25861 tcatgtgcag taatgtttat agttggaagt acaattttgt acaaaatggt ttcaaaagaa
    25921 aaagccaatg gtggtcattt tatagcacat gaatcagata tatttgatga agcagaagca
    25981 actaattcta agagagaaaa tggtcccaat ggtgtaattg ccttaattcc attgatagtg
    26041 tcaattttaa taattaatgt aaaagttaat ggaaaagcga tagtaccaat tgaggtggga
    26101 gtatttattg gagctttatt agtatattta ttgttaaata aatatcaaga taatgctaaa
    26161 atagttggac atgttggtga tgcatgtaaa actacagtag tagcaatatg taatacttgt
    26221 gctgtagttg catttggttc agtagtaaaa tcagcagttg gttttgactt catagttaat
    26281 gctatgacta atattcctgg tcctcctatt gcaggggctg cagttggaac tacagttatt
    26341 gcaggtattt gtgggtcagc ttcaggtgga ctaggaattg cagctccact tcttggacca
    26401 gtatttttag ccaagggtgt ttccgctgca gcattaacta ggactatggc aatttcatca
    26461 gcagcattag attcacttcc acacaatgga tatattgtta ctgtaacaaa tggtctttgt
    26521 aaagaaacac ataaggatgc ctatatgcca atattctggg taactgtttt aaccccattt
    26581 attggaacca tagtagcagt cctactgttt acaatgttcc cgggattgcc ataagagaaa
    26641 agataaaagg tataaattat attttgatgt tataaaatca attttcaaaa tatatacaat
    26701 aaatttcatg ttttaatact gtaaatatgt atttaaaata acaaattgac catataagga
    26761 ggaattgaaa gtgttaaata aaccattaga gggagttaag gttattgacc taacgtattt
    26821 tgtagcaggt ccagggactt ctaaaatatt agctgactgg ggtgctgacg ttattaaggt
    26881 agagccaagc tttggagacc caggaagaaa gacaggggca acaatgacaa tgccaataga
    26941 tgattataat aatccttttt acagcacata taattcaaat aaaagaggtt tgtctataaa
    27001 tcttaagagt gaaactggaa ttgaaattat ggataagctt ttaagcgaag caaacgtatt
    27061 tgtttcaagt tatagaacag gagcattaaa aagactaggt cttgattatg aatcattaag
    27121 taaaaagcat ccacacctta tctggggtca gataaatggt tttggcgatt ttggacctgc
    27181 taaggataat gcaggatttg atacagttgc cttttgggca agaagtgggg ctatgcttga
    27241 tatagcagaa aaagatacaa gtccaataaa tccacctata gcctttggag atgcaacaac
    27301 ttcttgtagt ttatctggag gaatatgtgc agctttgtac cagcaagcta agactggaaa
    27361 aggacaaaaa gtaatggcat ctttatttgg gcaagcaata tggaatgcat catctttatt
    27421 agcatctaca cagtttagtg atgagtatcc aaaaactaga aaaaatgcta tttcaccagt
    27481 aataaattct tatcgttgta aggatggaaa gtggatattc ttaagtattt tagagcatga
    27541 aagatatttt gaggcacttt gtagattatt tggaagagaa gacttaataa aagaagagaa
    27601 atttgcatct tcagtagaaa gtaagaaaaa tgccacagaa cttattagta ttttggatgg
    27661 tgaatttgca aagtttactc aaaatgagat ggttgaaaaa cttactgctg ctgatatagc
    27721 tcatgaaaaa attcaacatg ttaatgatgt agtgactgat gaacaggcta ttgctaataa
    27781 ctatatatat gaacatacaa gtaaaaatgg aaataagaca atgcttgcat ctactccagt
    27841 taaatttgga aatattgaag ttaacatgac ttgtgatgct ccacttatag gagaacattc
    27901 agatgagata ttgagagaac ttggatacag tgattctgat atagaggctc taattgaatc
    27961 tggaattgta acaatgaata aagaagcatg tgaaatatgt taataaacat gtgaagtatg
    28021 ttaataaata ggagggaatt ttatggattt tagattaaca gaagcacaat taatgcttca
    28081 aagagtagca aaagaatttg cagagaatga aatagcacca atagctgctg aaacagataa
    28141 aacaggtatt tttccaaggg agctttttag caaaatggct aaaataggat ttaatggaat
    28201 tggaacacct gtagagtata gtggttctgg tggggcagat atagaaaagg taattgttgt
    28261 tactgagatt gccaaaaaat gtgcagcatc tgcagcaata ttatcaatac acacaatata
    28321 tgctcaagct attttgaaat ttggtactga ggaacaaaag aaaaaatacc ttccaatgat
    28381 ggctgaaggt gggtgtgtag gtgcttttgc cttaaccgaa cctaatgctg gttcagatgc
    28441 tgcaagagct gcaacaactg caattattga tgaagaaact gatgaatatg ttttaaatgg
    28501 aactaagtgt tttatttctg gtggaggtca agcagaatca ttaataatat ttgcattgac
    28561 agacccatca aagggaataa agggaatgtc tgctatcatt gtcgacaaag gtactccagg
    28621 tttttcaata ggaaaaattg aagaaaaaat gggtattcat ggttcagaaa ctgcagagtt
    28681 aatatttgat aactgtagag ttccaaagtc taacttatta ggaaaagaag gcaaaggatt
    28741 taatatagct atgacttgtt tagatggagc aagaattgga gttggagctc aagctgtagg
    28801 aattgctgaa ggggctttag aagaaagtat caaatattct aaagaaagag tccagtttgg
    28861 caagccaatt tcagctttgc aaggtattca atggtatata gcagatatgg cgacaatggt
    28921 ggaatcagca aaattattag tatattatgc tgcagatttg aaagctagag gagaaaaaca
    28981 cactaaagaa gctgctatgg caaaatacaa tgcgtcacgt actgcaagag aggttacaaa
    29041 tttagccctt caaatccatg gtggatatgg atatatgaag gattatccat tagagagaat
    29101 gtacagagat gccaaaataa cagaaattta cgaaggtact tcagaaatac acaaagttgt
    29161 tatttcaaga gcagtactag gataggagga aaagaatgaa tatagttgtt tgcttaaagc
    29221 aagttccaga tactaatgaa gttaaaataa ataaagagac aggtactcta ataagagatg
    29281 gtgtaccaag tataatcaat ccagatgata gaaatgcttt agaagaagct ctaaaaatga
    29341 aagatgaact aggagcagtg attaaggtta taagcatggg acctcctcaa gctaaaagtg
    29401 ctttaaaaga agctttggca atgggagctg atgaagctta tttaatctca gatagagctt
    29461 ttggtggttc agatacttgg gctacatcta caattattgc tgctgctata gagaaggttg
    29521 gaaagtatga tgtaatattc tgcggaagac aagctataga tggagataca gctcaggttg
    29581 gaccagaggt tgcagaattt ttagggatac cacaagttac ttatgcgaaa gaagtaaagg
    29641 ttcaagatga caaattacta gtaactcgtt atacagagac aggggattat ttaatagaag
    29701 caaagatgcc agttttatta actgctataa aagagttaaa caatccaaga tatccaagtg
    29761 tcaaaggaat cttagaagct tataataatg gagatgcaaa gattactgta ttaacacttg
    29821 cagatttaga tgttgataca actcaaattg gattaaaggg ttcacctaca aacgtttata
    29881 agtcttttgt accagttaaa gacaagcata atgaaataat agaaggtata aataaaaaag
    29941 aaaaagctga gaaactaata gaaatactat tcgatttgaa actagtatag gagtgttaag
    30001 atgatgagag caaaagttaa tcaaggtata aatttaaatg attataatgg tgtttgggta
    30061 ataggagagc aaagggaagg taaaattaat cctgttacga tagaacttat tggtgaagga
    30121 agaaagcttg ctgaccaatt aggaaaagag cttgcagttg tgatagctgg atatgaagta
    30181 gagaaagaag tgaaagaatt acttcactat agtgttgata aagtatatta catcaatgac
    30241 cctcttctta aagatttcac tactgatgga tatgcaattt caattgcaaa tttaatcgaa
    30301 agaaaaaagc cagaggttgt attggttggt gctacttcta taggaagaga tatagcgcct
    30361 agaattgcag gtaaggttgg aacaggtcta actgctgact gtacaaaact tgaaatagat
    30421 tcaacagata ataagttatt acaaacaaga ccagcatttg gtggaaattt aatggccact
    30481 attgtttgtc caaaaaatag accacagatg tcaacagttc gtccaggagt tatggcaaaa
    30541 gctgtaagaa acgaatcaga gactggaatt ttagaagttg ttactccaga acttactgaa
    30601 aaaatgatta gaactagatt agtagagata cttccacaag aaaagaaatc tgtaaatcta
    30661 actgatgcta gaataatagt atcaggggga agaggattaa aaagagcaga aggatttgag
    30721 cttataaaag aattggctga taaattgggg gcagagattg gtgcttcaag agcagcagta
    30781 gattcagggt ggatagaaca ttctcatcaa gtgggacaaa caggaactac tgttagacca
    30841 gaactataca ttgcatgtgg aatatctgga gcaatacaac atttagcagg tatgagtgat
    30901 tctaaatata tagttgctat aaataaagat gcaaaagccc ctatttttag tatatgtgac
    30961 tatggaatag taggagattt atatgaaatt atacctgaaa tgatagaatc attaaatagg
    31021 taacttttag aaaacattga atatgtagaa tgataagtct tataatattg tattgtaaga
    31081 cttattattt ttgctgaagg ttatagtggg gagatgaaac aatgaaagag cagtggtata
    31141 aggatatatt tgcaagagta ttatctatga ctgatgatgg ttttattgtt gtaaatacaa
    31201 gtggagtaat aatagatatt aatgataaat attgtgactt tttaggaaaa gaaagaaaag
    31261 atataatagg acaaaatata caaagtatta taccaaatac aaaaatgcta gatgttatga
    31321 aaaataagta ttgtgaagaa ggagctatac atcattattc aggtggaaat acaaaagaaa
    31381 aaagtgtaat agtaagtagg tcttatgtgg aaaatgacaa tggagaagta gttgcaggag
    31441 tagctcaagt taaatttaga ttgcaatcat ttgatgtagc taaaaagcta atgtcagaat
    31501 atatggagct ccaatattat aaagagcagt ttaaggataa ctgtggtttt gacaagctca
    31561 taggtgaaaa tagagacttc atagaactta agaaaacagg tgtgaaagct tctaaaacaa
    31621 actttccagt acttttaacg ggtgaaacag gtacaggaaa agaagtcttt gctagagcaa
    31681 ttcacaataa tagcagcagg tcagataaac ctatggttag tataaactgc gctgcaatac
    31741 cagaagaatt attagagtca gagctatttg gttatgacga aggagctttt acaggagcaa
    31801 aaaagggtgg taaaaaaggt aagtttttag ttgcaaataa tggtacaatc tttttagacg
    31861 agataggtga catgcctttg accatgcaag caaaactttt aagagttctt caggaaagtg
    31921 agatagaacc agtgggaggt cttaaaacta taaagataga tgtaagagta atctctgcca
    31981 caagaaagaa tttaagtaaa atggttgaag agggattatt tagagaagac ctatactata
    32041 gattaaatgt aataaacatt catatgatgg aattgaagga caggcaagat gatattttat
    32101 tgctagcaaa ttatatatta aacaaattaa atgttgaata caaggaattg aaggttttaa
    32161 gtgataaggt taaaaattgt tttattaact atacttggcc gggaaatata agagaactac
    32221 aaaatgtaat aaaaagtgcc tatgctgtaa gtgatgatat ggtcattatg atgtgtgacc
    32281 ttccatctaa gatggataat atcagcagag tagctcaatg taatgtggat agcaattgtt
    32341 caattcatga aatggttgag aattatgaaa agagcttaat tattgatgtg ttaaggaagt
    32401 ataattggaa atgttctaag gcagcagagg ttatgggaat acacaagagt cttttatata
    32461 agaaaataaa aaagtatgaa attgaattaa ataattaaga taaattgaat taaggcaatg
    32521 ctaaaaaaac gcccttattc aaagcgaact gtataaactg ttttatgcag ttaaaacgtg
    32581 taaaagtaga tgtgaagtac taatgtcaat cattagtact tttttattat atttataatt
    32641 actatctata tttcagtatc ttttgatatc taagattatt atgtttaatt taggatataa
    32701 caaaaccctc ttagttatgg acagtttttt atattttaac tgtctactaa gaggggatag
    32761 tttagtttaa atttagagta aattttatct agcatttata aaaaatattt tgaatagctt
    32821 ttttgttatt taatcataat tttctacttc tttatcaagt tctttgccac aagaatcaca
    32881 cttatatata gcattgtatc cgtaatcata ttcatcaact attttctttt gtctaaatac
    32941 accaccacac tcacatagga aaccaatttt tataagtgta acgataaatt gtgattttat
    33001 aaattttata tcattacatt tatcgttagt gtaaaatgta taaatataag agttatcgca
    33061 ctcatctctt aattcaaatc tttcaatata tgtgttattt tttaattcct caaattcttc
    33121 tttagttaaa tatgattttt gttgcaattc atgtaaacgc ttaatttggt ccattattta
    33181 tatcctccca ataattttag tattttaagc ttagataagg aatctaagtt ttattaaatt
    33241 tatcccaagg aattatttaa atcatattta gttttattaa ttacaacata tataaattac
    33301 acgaaaataa aactaattgt aaaaataata tcctgtgaaa acctaaacta ctaacccctt
    33361 actaacccga aaactaaaaa aagaaaatat atccaaatat aagtgatata taaataaaga
    33421 tgtttcatat aaatttaact ttaaatattc tgacaataat atatataaat atattatagt
    33481 taaaatatac cacatatttt gggtattgta tactggaatt agagtaatta atttattttt
    33541 taacatacaa atgattttat gaaaaaaaac aagactcaaa aatttttata ttctttgata
    33601 gaataaaatt gagagtaaat ttattgaatt ttataaaatt tataaggggt gagtctaaga
    33661 tatgcatgaa gataaaaatg atatatttga atcaatagat actgaggatt ttgataataa
    33721 aacagaagaa gaatgtttag agctagtaag aaaaaatgga ttgaatttaa tatacataaa
    33781 aaatcaaaca gaagagatat gtctagaggc tgttagacaa aattgtaatg caataaaatg
    33841 tgttcagaat cagacagaaa aaatatgtat agaagcagtt aaacaagatt ggagaatgtt
    33901 agaatttgtg gaagagccaa cagaagaaat ttgcatgttg gctattaacc aagatggaac
    33961 agccttaaaa tatatagaaa atcagacaga ggagttatgc ttaagagcca ttgaaaaaaa
    34021 tggtgcggct ttggagtatg taaaagaaca aacagaagaa atatgtatag aagcagttag
    34081 aaacagcgaa tttggattgg caagagtgaa aaatcaaaca gaaaaaatat gtatggaagc
    34141 agttaagcat tgtagctata atctaaaatg ggtagaaaat caaacagaag aaatatgtat
    34201 ggaagcagtg agacaaaatg ggcttgattt gaaatttgta aaaaatcaaa cagaaacaat
    34261 ctgtttaaga gcagtgagac aaaatggaat ggcattagaa tttgtaaagg aacagactgt
    34321 tggtatatgc ttaaaggcag taagacaata tggaatggca ttaaaatttg taaaagaaca
    34381 aacagaagaa atttgtacag aagctataaa acaagataaa agagcactta gttttgtaaa
    34441 aggagataag gaaaaataca aggctctata tgataataat gaaccatttg caaagagata
    34501 tgttagaaat gtaatagaaa aagaaaacag agcattgatt aaaaaaggag aagaaaatgc
    34561 aaaaatcaaa attgcaggaa aactttatga tagaggcatg tcttttgaag atatatcaga
    34621 tatagttgag attgaaataa gcaagttaaa agtgagttta ggtgtttttt aaagtaataa
    34681 atgtaggtgt tttttatggg aaatagttaa taaaaaaata atcgactagc tctttgtagg
    34741 acagcaaaat tagtgatata atttgatgta taataaatta tttttattag ataaggggga
    34801 attaaagttg aagattacag tattatacca aacaagaagt ggaaacacag aaagggtcgc
    34861 taagctgata gaagatggag ctaaaaaggt ggatggagta gatgtaaagc ttatgagact
    34921 tgataatatt gatttagatt atttaaatga gagtaaagca gtaatatttg gaacacctac
    34981 ttatttagca aatacaactt gggaagtaaa aaaatggttt gatgaagatt caaaaaaagt
    35041 taatttagct ggaaaattag gagctgtttt tgctacatgt gattatatat gtggaggtcc
    35101 agatgttgcg atacttacta tagtaggaca tttgatggtt aaaggaatgc ttgtgtattc
    35161 tggaggtggt tctttaggaa agccatttat acatttagga catgtacatt caattgaagg
    35221 tccagagtta caagatgaga aggctataat atttggtgaa agaattgcta ctaaggcaaa
    35281 agaactattt gcttaatttc tatatgcata ataatgatta gagtaaattt agggttttaa
    35341 gggagatttg aagtaaaagt tataaaataa aaaattttcc ataaaattac tcaagatgaa
    35401 ttataatata attataaata aattatatta ttaaaatatt taaaatcaaa cgtcaatagc
    35461 gagggaagtt tatggtatgg catatagaat atgagatttt ttctgcaatt atagttattt
    35521 ttttaatggt atactttttt agaagtaaat ttatacctac tttacaaaac aaaatatatt
    35581 gtgcactttt aatattctca tttttattta taatttcaaa tattttaggt tcattttgtc
    35641 taaataatat agacaaaatt ccaattttta ttactttttt attgaatcag atatacttat
    35701 tgttattgcc aataccagca gcacttgtat cattttatgt tatggctatt atttatcaag
    35761 atataaggta tatgaaaaaa agattattat ttttatctat accacttata atttgtataa
    35821 tcttatctat tacaaatcct tttacgcata ttctatttgg attgtcaaaa gaaacaggtt
    35881 atatacgagg ttgtggatat gttgcaactt ttgcatcttt tttcttttat gcaatatatt
    35941 cagcattttt atcttttaaa tatagaaaag caatgcatga atcaaaaata tgggctatta
    36001 gaggttttct tatagtttca attgtggcaa ttatcttaca ggcaattttt ttacagtatc
    36061 ttcttacagg aactgcatgt gcatgtagtt tacttttgac atatctttct atgcagaatc
    36121 gaggtcttat tattgatgac cttactggtg tactcaatag acaatctttt attcaagagc
    36181 tagatttgaa tatcaatact gaacaatcag gatttattat tacaattgcg cttgatgatt
    36241 ttaagtttat gaatgaaact tttggaacaa aaaatggaga tattgcacta aaagaagttg
    36301 gaaaatattt gattcagata catgatgtaa accatgtata tagaataggg ggagatatat
    36361 tttctattgt tgtcaatagt caattaggta tagaaccaga tgatattatc agaaagatag
    36421 aagaaagatt taaaaagcct tggctagtag aagagataag ttttaattta gcaactagta
    36481 ttgctgttgt ttattaccca gaaaatgcag aaacgacaga agatgtaatt gttgctatag
    36541 atttctctat acatgaagca aaacgttctg agtcaaggcg agttgtttat gcagatgcgt
    36601 ctataagtga aaagataaag aagaaacatg ttattaaaaa ttgtttaaaa gaagcattaa
    36661 aaaatgatgg gtttgaagtg tactaccaac ctatattttc taatagcagg ggcaggttta
    36721 cttcagcaga ggcattactt aggctagaac acaaagagtt aggttttata cctcctgatg
    36781 aatttattcc aattgcagaa caaacaggat tgattaactc aattggatta gtagtctttg
    36841 agaaggtatg tagatttata gctagtgatg agtttgaaaa tttaaaattg gataatatag
    36901 ctgtgaatct ttctgtagta caatgtatgc aaaaaaactt agctgatgat ttactttatc
    36961 ttatgaataa atataatgta tcaccaacta aatttaaact tgaaattact gagactgtgg
    37021 ctatgggctc ttttaatata attaaagaaa caatggaaag attaattgat ttaggtgtta
    37081 agtttgcact tgatgatttt ggaattgctt attctggagt tactaatatg ctttctttac
    37141 cattttcagt aattaagtta gataagagtt tgatttggtc tatgaatgaa gatagtaggc
    37201 ataaacttac agttgagacg attatagctc ttataaataa attgaatatg aaggctgtag
    37261 cagaaggtgt tgaaaccatt aagtgtgctg aagatttaat agctatggag tgtgaatatt
    37321 tgcaagggta ttatttttct aaaccagttc cagaagatgt ttttaaaaat ttgttgaaag
    37381 aaaatacttt tacaagttaa caccctttat ttttttaatt acaactttgt tcattgacac
    37441 tgccagcgat atattataaa attaacacaa taataaatat taaatttatt atacatatta
    37501 ttaatattaa ttaatggagt aaagatttat tatatatttt atttaattat cctaaagtga
    37561 gaaaggaaga tgtgacatga ataaaaagaa aatattagtg ccaattgatg ggacagaaag
    37621 aagtatgcat tctttggaat ttataaaagg aatatttaaa aaagatgaag ttgaagtaga
    37681 aataatgaat gtaaaagaac tagtatttat tgatggcata tccctggctg aagagataaa
    37741 aaattctgaa aatttaggaa gaagaatctt ggataaagca gcagagataa tgggagagta
    37801 tgatgttaaa gtacatttta cttttggata tccaggagat gagataatta gaaaagcaaa
    37861 agaagataat gtagatttta tagttatgac aaaatctact aaaaaaggtc ttacaagaat
    37921 gataggttca gtaacagcaa gtgtagttaa acaagcaaaa tgtatagtga tgattgtacc
    37981 ggagtagaat tagaaaggta tttgaaatat gaataaaaag atagtagcag tttgtgaaag
    38041 tgaaagaagt ttaaattctc ttaagacagc tgcaaatgat ttaggatttg atttagttta
    38101 tgaaattcaa gaaaatgata atataaaaaa tgaactttct atttatgata tagaagatgc
    38161 atccatagtg ttatttgtta caagtgattc tatagaaagc atagagaaga ttgatagatt
    38221 tatagataga gaattttatg aagttgatcc aaagtatata atagaggatg caaaatctat
    38281 agtaagtgaa attttaatag acttaaatta atcgtgggtg tcgcactaag aatagagcga
    38341 cgccttttta tatattttac acaagaaata aagaggttgt atcaaaatat agataagact
    38401 ctttttcatt gttgaaatga tgaaaatata tttgatttca tacaaaaaag agtacctcat
    38461 aactaaaaag ttcactttga gacactccat ttttatatgt tagttgcatt taaggtaaaa
    38521 tataagttta atcatgttga aataactgaa ttacatcttc taaatgattg tacattgatg
    38581 tataacctaa gtaagagtct ttactatcaa atgccatcat tatcttgtta tggtggctaa
    38641 tcatatcctt gagagaattt ctatatttaa aaccgaagta ttggttagaa gaaactgttt
    38701 taggtattaa ctcataatat tttataaaaa ttggattatg agaagctaaa attatctctt
    38761 tgtgaaattg caagtcagca tcataacatt cttctatcaa accatgagaa accttatcca
    38821 attcctctat tgactttagg taagtatatt ttattttttg aagttcttta gatgtagcat
    38881 ttaaaattgc tagtttagta gcttcacttt cgataagttg tcttgctctt aaagattcta
    38941 cataaaattc ttgtatgtta tgatttttat agatttcacc atcatcaaat aggtcaactt
    39001 ttttaaatcc ttcaactata ggacttgagt aaggcacagc tctaagtatt ccttccttac
    39061 agagtatatt tatggcattt cttacgggta ttctacttat gttaaatagg tcagcaatct
    39121 ttctctcact tggtaattta ctattaattt taaaagttcc atttagaatg tttaatttaa
    39181 tataatcaat aattttttca gtggcattta agcttttcat aaaaactcct aaacatataa
    39241 tttttttatg attgtattat atcatagtta agagttaaat ctactttttt ataatatgta
    39301 taagacagga tagaggccaa aatagctata atagccaaga taaagtatcc atattgtatt
    39361 gaagtatatt ccgatatttt accaactatc attggaaata cagacattcc tatagcataa
    39421 attgcttgaa aaaaacccat tgcagtagat ttctgatgtt ttggtacatt tttcatagac
    39481 tcgctagtaa ggtaagaaag gagtatacct gtagatatac ctggtagcac ttgtagtaca
    39541 aatattatat taatattttt tattgttgga actaaaaagc aataggctga aactaaaata
    39601 aaaactaaag gaatccaaaa ttttggccca tacttagtac aaaatttagt agaagcaaag
    39661 ccagaagaag caacagcaca caccatgtaa aatatagaag atagacctat gagggtagaa
    39721 ctagaaccta agtttttaag aatttgagta gtaaatgaca ttgttgtaga catttgaata
    39781 ccctgttgaa ttaatgccat aaaggaaaaa aatacaagtc ttttatcctt gcagatagat
    39841 aaatattttt taattgatat tttttcaggt attttttttg aattatgatt ttttatattt
    39901 aaggctagaa ttgttccaat aactgaagac aatacactca tgatacataa ggctgtcatt
    39961 cctaattttt cataaaataa agtactcata acaaatccaa tcaacattcc tagattgttt
    40021 accaatatta tcgtacttgt agctctttgt tgatgttctg tagaaaaaaa gttggtatag
    40081 tgaaccatgt aagaaatcca agttgatgat gatattccag ataaaatgtt agctataaga
    40141 aaaccattgc ctccaggaga gaagactctt ataagagatg ctaaaccagc aaagaaaatt
    40201 ccagacagta taaatatttt atgtttattt atggaatccg caaatactcc aacaggaagc
    40261 cttaataaac attgtgagat tccatatgca cctattatta taccaatcat atttgtagat
    40321 acattacatg cacttaaata tggattttgg aaaggtatat atacatattg tgaaaaccag
    40381 aaaaatgtaa caatagaaag taaaagtaat ttttgattag atattttttt catattatcc
    40441 tccgtatggt atttttataa tgcaaataat ataccatttg gattttttaa tcaagtactt
    40501 tatctattaa aaaaaattta aatatataat cattatttac aaaaaaatta tactttatag
    40561 ttgaattaag aaaacgctta tgatactata aagttaacaa atttgaatat ataacagtag
    40621 acgtgttact ggtaatgcag gcaagatcga atcaattttt atagattatc tatagagtac
    40681 tatgggtata atctatttat ttggttcgat ttttttgttt aattggccat aaaacaaaaa
    40741 gcctatagtt gaaaatggag gattttttat ggattataaa aatatagctc aagaaattct
    40801 actaaatgta ggaggaaaag agaatgtaaa tgaagtgact cattgtatga caagacttag
    40861 atttaaagtc aaaagcgctt ctaaagtgaa taaggataaa ttatctaaaa ctgaaggagt
    40921 aataactgtt gttgagagta tgggtcaaat acaagttgta attggaaata aagtaaaaaa
    40981 agtatatgat gaggtcataa aaattgtacc tcaatcaaat aacgtaggta aaaagaatga
    41041 aagtcaagaa aagcaaggta taattaattc tatattatca gcagtagctg gtatatttac
    41101 acctacaata cctgctatag caggggttgg tatgattaaa ggtattttat cagttcttgc
    41161 tatgtattat atgaataaaa atggagtaga tataaaagaa acgcaaagct atattatctt
    41221 aaatgctatg gctgattcta tattctattt tatgcctatt atactaggat atactgcggc
    41281 taaagtattt aatgcaaata aaataatatc tatggtatta ggagctacac tttgttatcc
    41341 aacttttact gctttgatgg caggtgaaga atctgttaga tttttaggat tggcagtaac
    41401 aaaagctacg tatacatcat ctgttattcc aataattatt gctatatggg ctctttcata
    41461 cgttgaaaaa gtattagaaa agtatatacc tgaggtaatt aaaataataa tggttccaac
    41521 tttatcattg gttgtaatgt tacctgcaac attattttta tttggaccaa tcggaattta
    41581 tataggaaat gtcataaact tttcttataa gtatatttat gaattaagcc cagcattgtg
    41641 tggagcattt gtaggtgggt tatggtgtgt attggttata tttggtgctc atagagcatt
    41701 attaccaata ggtataagtg atgttgctca aacaggaaga cagaatttat tggcgtttgc
    41761 aggatctgca aacttttcac aagcaggggc tgctctagga gtattcttta aaactaaaaa
    41821 tcaaggacta aaaacaattt ctatgtctgc aactataaca gctttatttg gtataactga
    41881 acctgcaata tacggtgcca atcttagatt aaaaaaacct atggtatgtg ccgtaatatg
    41941 tggtgcattt ggaggtgcga taatgggaat gggtggagct tatggtaatg catttgcaaa
    42001 ccaaggtgtt ttaactatac ctgtatatgc tgaagcaggt gcattaggtt tcttaagtta
    42061 tctaggtggt tgtgctatag cattttttgg ttcagctatt tcaacttatc tagttggatt
    42121 tgaagatttg gaagagtctt ctaataagga aaattcatct ataaaagtag aaacgaagga
    42181 tggtgttatt gatataactt cacctgtaga gggcgaatgt atagaattgt cagaagtaaa
    42241 ggatgatgta tttgcttcaa aagctatggg agaaggtata gctgtgttac ctacaaaagg
    42301 agtaataaca gcccctactg attgtgaagt agcttcttta tttccaacat tacatgcaat
    42361 aggattaaaa ttagataatg gagcagaaat gttaatacat gttggaatca atacagtaga
    42421 gttaaatggt aaatacttta caaaacatgt taatcaagga gatttagtga aaaaaggtga
    42481 taaacttata tcgtttgata tagataaaat aaaaaaggct ggttatgatg taactacgcc
    42541 agttatagta aacaatacat ttgattaaaa aatagctact aactcgtgac tttag
//
LOCUS       DAGSWI010000019        42474 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.019, whole genome shotgun sequence.
ACCESSION   DAGSWI010000019 DAGSWI010000000
VERSION     DAGSWI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.019"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            71..586
                     /locus_tag="MJF80_000928"
     CDS             71..586
                     /locus_tag="MJF80_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBZ0279601.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            635..1138
                     /locus_tag="MJF80_000929"
     CDS             635..1138
                     /locus_tag="MJF80_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBZ0279602.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            1135..1785
                     /locus_tag="MJF80_000930"
     CDS             1135..1785
                     /locus_tag="MJF80_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279603.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(1920..2699)
                     /locus_tag="MJF80_000931"
     CDS             complement(1920..2699)
                     /locus_tag="MJF80_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBZ0279604.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(2811..3062)
                     /locus_tag="MJF80_000932"
     CDS             complement(2811..3062)
                     /locus_tag="MJF80_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBZ0279605.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            complement(3391..4260)
                     /locus_tag="MJF80_000933"
     CDS             complement(3391..4260)
                     /locus_tag="MJF80_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBZ0279606.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            complement(4598..5359)
                     /locus_tag="MJF80_000934"
     CDS             complement(4598..5359)
                     /locus_tag="MJF80_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279607.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            complement(5375..6910)
                     /locus_tag="MJF80_000935"
     CDS             complement(5375..6910)
                     /locus_tag="MJF80_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBZ0279608.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            complement(7324..9564)
                     /locus_tag="MJF80_000936"
     CDS             complement(7324..9564)
                     /locus_tag="MJF80_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBZ0279609.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWNWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEAEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            complement(10091..10891)
                     /locus_tag="MJF80_000937"
     CDS             complement(10091..10891)
                     /locus_tag="MJF80_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBZ0279610.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGIHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            complement(11282..12157)
                     /gene="hslO"
                     /locus_tag="MJF80_000938"
     CDS             complement(11282..12157)
                     /gene="hslO"
                     /locus_tag="MJF80_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBZ0279611.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            complement(12440..13186)
                     /locus_tag="MJF80_000939"
     CDS             complement(12440..13186)
                     /locus_tag="MJF80_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBZ0279612.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLAKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            complement(13173..13418)
                     /locus_tag="MJF80_000940"
     CDS             complement(13173..13418)
                     /locus_tag="MJF80_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBZ0279613.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            complement(13549..14667)
                     /locus_tag="MJF80_000941"
     CDS             complement(13549..14667)
                     /locus_tag="MJF80_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBZ0279614.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYLKLLKYKEAID"
     gene            complement(14873..16066)
                     /locus_tag="MJF80_000942"
     CDS             complement(14873..16066)
                     /locus_tag="MJF80_000942"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBZ0279615.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            16389..17270
                     /locus_tag="MJF80_000943"
     CDS             16389..17270
                     /locus_tag="MJF80_000943"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBZ0279616.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            17695..18696
                     /locus_tag="MJF80_000944"
     CDS             17695..18696
                     /locus_tag="MJF80_000944"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBZ0279617.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            18813..19700
                     /locus_tag="MJF80_000945"
     CDS             18813..19700
                     /locus_tag="MJF80_000945"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBZ0279618.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPSYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            19767..20516
                     /locus_tag="MJF80_000946"
     CDS             19767..20516
                     /locus_tag="MJF80_000946"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBZ0279619.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            20740..21456
                     /gene="dapD"
                     /locus_tag="MJF80_000947"
     CDS             20740..21456
                     /gene="dapD"
                     /locus_tag="MJF80_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBZ0279620.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     NNGSYIFVGDYDDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(21908..22897)
                     /locus_tag="MJF80_000948"
     CDS             complement(21908..22897)
                     /locus_tag="MJF80_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBZ0279621.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLRMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            23337..24092
                     /locus_tag="MJF80_000949"
     CDS             23337..24092
                     /locus_tag="MJF80_000949"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBZ0279622.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            complement(24599..26275)
                     /gene="bclA2"
                     /locus_tag="MJF80_000950"
     CDS             complement(24599..26275)
                     /gene="bclA2"
                     /locus_tag="MJF80_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBZ0279623.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGPTGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGVNGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(26633..27172)
                     /gene="hpt"
                     /locus_tag="MJF80_000951"
     CDS             complement(26633..27172)
                     /gene="hpt"
                     /locus_tag="MJF80_000951"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBZ0279624.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(27500..28807)
                     /locus_tag="MJF80_000952"
     CDS             complement(27500..28807)
                     /locus_tag="MJF80_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBZ0279625.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDVAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(29231..31006)
                     /locus_tag="MJF80_000953"
     CDS             complement(29231..31006)
                     /locus_tag="MJF80_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0279626.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKVDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(31003..31140)
                     /locus_tag="MJF80_000954"
     CDS             complement(31003..31140)
                     /locus_tag="MJF80_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279627.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(31218..31787)
                     /locus_tag="MJF80_000955"
     CDS             complement(31218..31787)
                     /locus_tag="MJF80_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBZ0279628.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(31971..32606)
                     /locus_tag="MJF80_000956"
     CDS             complement(31971..32606)
                     /locus_tag="MJF80_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBZ0279629.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(33274..34155)
                     /locus_tag="MJF80_000957"
     CDS             complement(33274..34155)
                     /locus_tag="MJF80_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBZ0279630.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(34275..35282)
                     /locus_tag="MJF80_000958"
     CDS             complement(34275..35282)
                     /locus_tag="MJF80_000958"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBZ0279631.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(35302..35784)
                     /locus_tag="MJF80_000959"
     CDS             complement(35302..35784)
                     /locus_tag="MJF80_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBZ0279632.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GADGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            complement(35802..36269)
                     /locus_tag="MJF80_000960"
     CDS             complement(35802..36269)
                     /locus_tag="MJF80_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBZ0279633.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(36284..37042)
                     /gene="prdD"
                     /locus_tag="MJF80_000961"
     CDS             complement(36284..37042)
                     /gene="prdD"
                     /locus_tag="MJF80_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBZ0279634.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(37122..37847)
                     /gene="prdB"
                     /locus_tag="MJF80_000962"
     CDS             complement(37122..37847)
                     /gene="prdB"
                     /locus_tag="MJF80_000962"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(37395..37397),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBZ0279635.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(37885..38169)
                     /locus_tag="MJF80_000963"
     CDS             complement(37885..38169)
                     /locus_tag="MJF80_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279636.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(38250..40130)
                     /gene="prdA"
                     /locus_tag="MJF80_000964"
     CDS             complement(38250..40130)
                     /gene="prdA"
                     /locus_tag="MJF80_000964"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBZ0279637.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(40559..42319)
                     /gene="prdR"
                     /locus_tag="MJF80_000965"
     CDS             complement(40559..42319)
                     /gene="prdR"
                     /locus_tag="MJF80_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HBZ0279638.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
BASE COUNT        13538 a         7578 c         5142 g        16216 t
ORIGIN      
        1 cgtaagattt attatctgta ataataaata tatcgaatcg atatatatct aattgatacc
       61 aggaggtaat atgttagaat acattatttt aggattttta atggaaaaag aattaagtgg
      121 atacgactta aaacaaataa tgtcagaaag tacctcgtac ttttttgacg ccagctttgg
      181 aagtatctat cctgcactta aaagattaga aacaaaagga tatatacact atcacgaagt
      241 tatagatggt agcaaattaa aaaaactata ttccataact aatactggta aagaagtatt
      301 tcttgaatgg ttaaagaaac ctataaattt ttctaaaaca aagcaagacc accttgttaa
      361 tatattcttt tacaagtatc tacccaaaga aataattgaa acaaacttaa aaatgttaat
      421 aactgaagta gaattacttc taaataaatt aagtaaacaa aaaaatgatg cagaagaaaa
      481 tcattgtgtt gaccaatata catatatgta tgcaacaatg atttatggag ttgactatta
      541 taattttgtt attaattggt gtaaagattt actgaaaaat ctataagata taatttttag
      601 aagtaaacat acataataaa aggagatgga ctttatgagg cttttaatac atgatttaac
      661 tgacaaagaa ttagaaaagt tattacccaa acttgatgac aatataaaaa tacttccaaa
      721 cacaaaagaa atccacaaat gtattggttg ttttggttgc tggattaaaa ctccttctga
      781 atgtgttgta aaagattatt acagcaacat gggtaaactt ataaatgact ctgatggaat
      841 tattataata agcagatgct gttacggagg gtttagccct tttgtcaaaa acgtacttga
      901 taaaagcatt aattatattc ttccatactt tgtaataaaa gataatatga tgcgtcataa
      961 aagcagatat gataaacaga taaatttaaa agctatattc tatggagatt gtattacaga
     1021 agctgaaaaa gaaactgcta cgaaattagt aaatggaaat gctataaatt tcaatataaa
     1081 agaacactct gtttgtttct atgaaaatat acaagatata agaggtgaat ccatatgaat
     1141 atagtaatga taaatggtag tccaaaagta aataaaaata attcacagta ttttttatct
     1201 gaattaaaac aacttattca aaatgataat aaagttttta catttaaagt agatagcaca
     1261 tctaagtatc ctgacgctct taatgccata cttaattgtg atagattaat aatcgcatct
     1321 cctctttatg tagatggtat tccatctcat ttacttcatt ttttagagaa atgtgaaaaa
     1381 tttttaaaaa cacaaaataa aaaacctatt ccagtgtatg caatagttaa ctgtggattc
     1441 aatgaaggtg aacataatca tttggcaatt caaatgatag agcattggtg taaaaaaatc
     1501 aactttacat gggttcaagg tattggaatt ggagctgggg agatgtttgg ttcactagaa
     1561 tccgtacctt tgggtaaagg acctaaaaaa gatttaggaa ttgcattaaa agaactttca
     1621 aaaaatatac ttagtgataa tatacatata gatgattcca acaaaagtac tatttttatt
     1681 tcacctaact tccctagatt cctatataaa cttatgggga atcatcaatg gaatactcaa
     1741 gctaaatcaa atggattgac taaaagtgat atactaaaga aataaaattt tacttcaaca
     1801 aatactaaaa accgtcaagc acgaataaac tattcatctt gacggttttt tatgttattt
     1861 atattcacta gacatgggat aattcactat aacattgatt atctagtcta atatttatct
     1921 tattcttttt gtaaaatcca agttgctcca acaaggtcct gctgtaaatc agtatattca
     1981 tttttttggc taataacata ttgttcaaac atatcttttt gagaaggata ttttttaatt
     2041 agctcatttg cacgagttat aatccaattc atttcctctt caacatcatt ttcaagttct
     2101 aaacatggta tacattcaat tagcttcaga cctaattctt tgaacaatat acaccactca
     2161 ttgtaagtgg gatacaccct ctcaaaatta agctgttcat ttgtagattt atctactaca
     2221 tatgcatcat caattaaaat gaacccctta ctttttattg taggtaatag cttacttata
     2281 gtttctttat aattacctaa aatatcccct acagctccaa aaatcacaca atcataatca
     2341 tgctcaattt ttacagaact attagcatca ttcatttcaa aatcacacaa agcatcaacc
     2401 ttatattcaa tagcctttct tctagcataa tcaataaatt caggtatcaa atcaatacct
     2461 ttgatatgta tattcaactc ttttgcaagc ttcacagata cagcaccttt tccacaagct
     2521 aaatccaaaa cttttgtatc ctcattaaaa ggtatatgct ttctaaataa ttgtaacatt
     2581 tgttctggtg aacttcctaa ttcccaaaaa tcttgtaata aataaggcaa ataaggtaaa
     2641 atatcagtag tatcagaagt tagtgactct gccaattttt cttttacagt atcattcatt
     2701 ttttcaacct ccaaaattta aattattatt caaaagtaat attattttat atacttcttc
     2761 ataggtacta caaacagtgg aaaatgcttt tgacctttgt attttgcaat tcatttcttt
     2821 tctttatgag ctgtaataat tgtataactg tgagcgttta ctgttataat acaatcatat
     2881 acacttatat accagttttt tccctttcta ttaataatgg catttggaga ttctatttta
     2941 gttttacacc agttcactac atcatttgtc tccaaagaaa gatttttctt aattcttaca
     3001 acgcctaact ctgttgtatg caatctatct aggtttttaa gtaattcgtt ttctacattc
     3061 atttttatta aaacacctta tcctttgcaa aattaaatct attatttagt actattttat
     3121 catagtattg tgtattttat cataatctta tagtaaaact atttctttcc tacagattat
     3181 aaattattta tgaatacaca ccattataga acttttccta tgcaaaatta aatctattat
     3241 ttaacactat tttgtcatag tattgtgtat tttatcataa tcttatagta aaattatttc
     3301 tttcctacaa actataaatt atttataaat acatactgct gtaaaacttt gcctatataa
     3361 atccatctac tttatacata ataccttctt ttaaatgatt ggaacccaaa actgaatttc
     3421 actcaaacca ttctcatcag ttttttctcc atattttaca aagtcatata aattattttc
     3481 attaaactgg caacttgaat tagggaacca ttcttgatag atatattcta caacattttg
     3541 catactatcc tcatttttcc caataaaagt aaaaacaata taattacttt tcggtaatac
     3601 gaatttttcc attccattag gtaatttgcc aaaagtagct acttgagcac ctgcaatgta
     3661 tgtaaatgta ggagtattct caatctcttt aaaattgcta taatcactaa ctccaattaa
     3721 aaaacctaaa tctaccttat tttctatcat ctctttattt ttatgcaaat ccctccagca
     3781 ttttccaata atatgaaatc cttttcttac tttaccagaa tagccaacaa tatttatatc
     3841 tgcacaatta acttttttaa catctaaaac catatctcca cgtaattttt ttctattatg
     3901 aatatcaaat tttaattgta gtggtaaaaa atcttgatgt cttctaaaaa aattaggact
     3961 acgtttaaac atttttttaa accctcttga aaaagagcgt tgtgtatcat accccgagat
     4021 taaagcaatc tcaataattg gtttttctgt aaaaattagc attctcgctg cttcagtaag
     4081 tctacgtctt tgtatataat tatgtacact aaatccgaca actgatgtaa acattctatg
     4141 taaatggtat tttgaatagc ctatctcttt agaaattaca tttaaatcta acatctcatc
     4201 taaaagatgt ttttctatat aatctaaagt tttttcaatg gtaatcaaat tgttatccat
     4261 atagcatccc tcctatttta aagatagcat attattttat tttgtttttc acattctttg
     4321 ctaatatttt gatttatttt atggcacaca aaagacactt ctatttgaag tgcctatcta
     4381 ctctgtatat tgagcttctt atttcaattt attataatct tcaacagata caggttctag
     4441 ccatttattg aaagcacctt cagcagttac ctcgtctaca gccaactgct aaatgccctt
     4501 ctatacctta atctaacttt tccacttcaa gtatacaact tgaagttagc tctaaatcaa
     4561 tagtttttat cacaacaatt tgattttaaa tttaaattta ttcttttaaa ttatcatcat
     4621 ctattataga ttttcttata ttcaataact ggtctacaaa ttggttagca ggagtattta
     4681 aaatattctc aggagtatca tactgttgaa caactccatg atttaatacc aaagtctttg
     4741 ttcccaaaat caaagcttca taaatatcat gcgtaataaa gataatcgta actcctgttt
     4801 ttttatgaat ttcttttatc tctttttgta attgctttcg tgtaatctca tcaactgcac
     4861 caaaaggttc atccattaat aaaatttctg gagaagaagc taaagctctt gcaatcccta
     4921 cacgttgttg ctgaccacct gacaattctg atgggtaacg cactttcata tcatttggca
     4981 atcccactat ttccatccac ttgtttacag cactttttac ttctaattta ttctttttac
     5041 ttaggagatt tggtacatat gaaatatttt cttcaacagt catatgagga aaaagaacac
     5101 ttccctgtac acaatatcct attcttcttc ttaatttaat taagtcctcg tttttgatat
     5161 ctgtttggtt tatcaaaata tttcctccat ctggcaaaat cagaccatta atcattttta
     5221 agagtgttgt cttaccagaa ccagacgaac caattacagt caaaaactct cctttttcta
     5281 tatctaaatt aagattatct attatagttt tatcattata ttgtttttta atatttttaa
     5341 attgtataat aggtgtcatt atatttcctc caatttataa aagacctttc ttattcagaa
     5401 actcacgagc aacttctcta ggttctaaat tgttgttttc tacgtcataa ttcattttag
     5461 ccatttcttt atcagtcaaa atatctccaa ccatcttaaa tacctttgat agttctggat
     5521 tttcctctaa tacttccaaa cgaacaacat ttccacattg ataagatggg tagaaatttt
     5581 tatcatcttt tagaactaca acatctgaaa cactatactg accatcagtt gtaaatatag
     5641 gcattacgtc aatcttacct tcattgatag cttggtattt taatccaata tctaaatcaa
     5701 ctgtatcttt aaagttcagt ccatatgtat tacacaatgg agtatatcca tcctctctct
     5761 cataaaagtc atattcagct ccaaatgtca atttagaaga tacatctttt aagtccgtta
     5821 tattttttaa attgtattta tctgcaactt cttttctaac tgctaatgca tagctattag
     5881 aaaaaccata cataccagtc cattgcattt taagtttagt tttgtattcc gactgtagtt
     5941 tactaaacat actttcagta tataattcat ttttcttaag taccatattc cagcctgtac
     6001 ctgtatactc tggataaatg tcaaattctc cattctccat agctggctgg atattagatg
     6061 ttccccctcc tacaccttgt gtaatttcca cgtttagacc tgtgtttttt tcaattaaaa
     6121 tatccaacat ctcaccaaga atatattgtt cagtcatagg tttagtagca atatgaatag
     6181 tttgagtctt tgaaggatta aataaataag acgtaaagat aattactata agaataaggc
     6241 ttcctagaat ggcaaaaact tttttcctat ttttcttttt tctttttcgt tgaaaaaatt
     6301 tttctaccag ccctaaaata aaatcaaata ttaaagctaa tagagcaata agtatacttc
     6361 cagtaattgt cattattgtg ttatttgtcg tgattcctct atatatggct acacctaggc
     6421 ctccagcacc tataaatgaa gcgataccag ctaatgcaat tgtcataatc accatatttc
     6481 taataccaga cataataaca ggtagggcta acggaagttt tattttatac aatatctgaa
     6541 aatctgtact tcccattccc ctagctgctt caagaatatt tttatccaca ttagtaattc
     6601 cagtatatgt atttctaacc attggcaata atgcataaat ggtaagtgca ataacagcag
     6661 tagtatttcc aattccggaa aatggtatca gaaatcctag catagaaata gatggaatgg
     6721 tgtatagaaa attgatagtt cccaatatag gtttagaaga attatgaaat tcacttacaa
     6781 taataccaat tataccaccc aaaacaatcg caattattat agaaataata gaaatcatca
     6841 aatgttctat ggatagcttt ataaaaaaat cacttctttc agataataag gttagaatat
     6901 catttatcat ccatctctct ccttttataa gttttactta acaagattta aattttaatg
     6961 tagttaatct ttattgttta tacatcagca aatcgtcatc aaattatttc acaaattata
     7021 tattaagatt ataatctgaa cctcccatga ctgaagtcac aagtgtgtat agccattttt
     7081 taatcaaact tgcaagtaga cacaaaaaag taactattag agactccatc ctcgatattc
     7141 tccataaaaa taagaaaaaa ggtcagaacc gttaaaatac taaaatttaa acaaatttct
     7201 gacactttat ttaattaaat ttgaatattt ctcacattat ttattactgt taagtttaat
     7261 tatcatttta tatacccatt atatttatta aaatatcatt ataattttat tatattttat
     7321 ttattatact atatattttg ctgttaatga tattttattg ttacataatt gttttggaat
     7381 accttcaaat attactctcc cgccttttgt tcctccttca ggtcccagct caataatcca
     7441 atcagctctt ttaattacat ctatattgtg ttcaattatt atgaccgtat ttccattatc
     7501 tacaatctct tcaattatac ttataaaatt ctcaatatca gccatatgaa gacctgttgt
     7561 tggttcatct aatatataca ctgaactttc cttatttaat tcactagcaa gctttaatct
     7621 ttgacactct cctccagaca aagtatcaag agtttgtcct aaagtaaggt atccaatacc
     7681 cattttttca atacattgta atttcgtttt aatctgtttt aaattaaaaa actcaattgc
     7741 gtctaaaaca gacatttcaa gaacttcaat tatattcttc ccttgaaacc tatattctaa
     7801 aacttctttt ttaaattttt taccttcaca aacattgcag gtactcttta tactctccat
     7861 aaaagcaaga ttcatttcta ctattccaga acccttacag ttatcacaag ctccttctga
     7921 atttgaactg aataaagaag catttacgcc atttgttttt gaaaatatgc ttcttatgtt
     7981 gttcataatt ccactatatg ttgccaaact cgaccttgaa tttgctgaaa cagggctttg
     8041 gtctataagt acagcactag gattttgctt taaaaattca tccttaatca acgtactttt
     8101 acctgctcct gctactccag taactaaagt caatacacct ttaggtatct taatcgatac
     8161 attttttaaa ttgtttttat tacaattttt aacttctaaa tatccactat gttctcttac
     8221 actttcttta ataggaagaa atttacttaa agcattccct gtcaaagtac cacttgtaag
     8281 tagattttca taactgcctt catacacaac ttctccacca tacttacctg cctttggtcc
     8341 tacatctaca atatggtctg caatcttaat aacatctggg tcatgttcaa caactataat
     8401 tgtattccct ttatcacgca atttttttaa aagattattt aatttataca catcttttgg
     8461 atgaagtcct acacttggtt catcaaaaat atacattaaa tctacaagat tactatttaa
     8521 atacttaacc atctttatac gctgagattc ccctcctgat agagaagatg tctctctatc
     8581 cagtgttaaa tatccaagac ctatatctat gatactgttt aacttttcta ttattccttt
     8641 aattaaaggt tgtgcttctg cctcatctat acttttaata acttcagcca attcatcaat
     8701 ttgcatacta gttaaatctg ctatattata accattaatt aaagacctat atactctttc
     8761 attaagtctt cttccatcac atataggaca tctatcttct ataagaagtt tactcaattt
     8821 tttcttagta gcttcagata tttctccctc tctacttaag tataggcgat taaatttaac
     8881 cattagtccc tcatatgtca tgttagtttg accagcattt tctatcttaa tcttttctgc
     8941 tttaccatgc aaaaactttt ccaattctac ttctgaataa tcacatatcc tcttattaat
     9001 atcaaaaaat cctgattcag cgaatatatt ccaattccaa gaacccactc caaaccctga
     9061 aagcaatata gcaccttcat ttaatgattt attcatatca agaatttggt tcatattagg
     9121 aactagcttt tttccaagcc catgacagtg tgggcacata ccatcaatat tattaaaaga
     9181 aaacatattg gcattaccta tatactcact accaaacctt gaatataaag ctcttaaaaa
     9241 ggaatttaca tctgtaatag tacctagggt tgaccttgaa tttccaccaa gtctactttg
     9301 gtctatgata attggtgttg acaaattttc tatacaatca gctttaacct ctccatactt
     9361 aggtaaaaaa cttcttacaa atgtactaaa cctttcattt agttggcgtt gtgattcttt
     9421 tgcaattgtc tcaaatacaa tagatgactt tccagaaccc gacacccctg tgaatatggt
     9481 tatttttctt tttggtattt ttaaggaaat attgttcaaa ttgttctctt tgcaaccttt
     9541 taaaattata aattcattgt tcatatttta cctcctgcat tatttttctt taaattattt
     9601 agagaaaatc gtaataatag ttgttagtta tacataagtg aaatactatt ttattagatt
     9661 actccttttt ttataaacac aggtcaaatt atagcatgta gattttatct ggtagtatag
     9721 tgtagggttt gatttttata aaaaatacag ttattttatt taagcgtaat tgcatctatt
     9781 ctacctaaat atcatcatta tatatgagtg ttaaattata taaaattctt aagttaataa
     9841 tctcaaaatg gtattagtta cataaaaaaa gtatcttgcc tatgatttta aaaccataaa
     9901 taagatacta tgttttacac tgtttcttct ttcacaaata attctataca attactcaac
     9961 ttaactttaa aattttaaac actaaaaacc tctatcttta aatctttaaa taaaactaaa
    10021 tttccaatac cttcaaaact atagaaaatt ccaatcaact tatcaaacat aacttactac
    10081 ttataagaaa ttaatcttca cttaatacag acgtaagttc agggtcattt agaagatgtt
    10141 caaaataact atgtgccgaa atatatccta taccatacat tttacgcttt gaatttacaa
    10201 ccttatattc ataacaagca ctcttaacct tattgtagtc atcaaaatcc attgctttat
    10261 agatagctct ttcatttctt ggtatatact ttacatattt tggcttaaca aaatacaagt
    10321 catcttttat cttaccttca cgtttaagtt tctttaaaac ttccccattc tttctatgca
    10381 ttatatggtc atcatactta tagtgatggg ctatatgggt cacattgcct ttaaacttat
    10441 gttcaaaatc caatatagtc ttttccatta attcataatg tatgccttcc tttttatcta
    10501 tatctgcaag tataatcaca ttttgaggtt tcactccaag ctctctcaaa gctgatttaa
    10561 actcgttatt tctaagttct tccttttgct tcctagttaa atttctaaat tttgtgttag
    10621 ctttgaaaac gttaactcca gaaccatctg atactaatac cacatataca ttatcaacac
    10681 cacactgttt tatagcatct acaatggcac ttcctcccca taaaacctca tcatcttgat
    10741 gttgaggata aaacactgct gctgatttaa attttctact attactctca ggaataggta
    10801 tttcaagttt tccgttaaaa ttctttgcaa ttaaaaatat catcaataat attaatatac
    10861 ttattcctat gtacttcgtt ttactttcca tatttaaaat tttctccttc ttctgaacct
    10921 cccatgatta aagtcacagg gttactactt catagaattt cgtataatca actcaaatat
    10981 cttataaacc tttgtgtaaa tcaagtctaa atatacaaat tattttacaa tattttattg
    11041 tttcatacaa tctttgacac gaactataat aaaataaaaa agccttctaa ctgcaacatt
    11101 gcaaacatta cagccagaaa gcttcttaat tcttaaaatt tattaagctt aaaaatttat
    11161 taaacttatt ttttaatata aatatgtcta ttattaataa attatacttt aattactata
    11221 tcaatacgac tttactatat tactataaca ttacttatat caatacaact atttttatag
    11281 tctacatttc ctttaaaatt tcaagcaact cttcttcact atacatatac ttcttattac
    11341 agaactggca accaacttct gcttttttat cttcttctat tatttgagcc aatgattttt
    11401 taccaattgc aaccaaagca gtttttactc tttcttttga acattcacac ataaacccaa
    11461 cttcacaggt atcaagtatt ttaggttcca aaccatccaa aacaatattt aatatatcct
    11521 ctggtgattt tccttctttt aacatgttag ttatagaagg taatttagct acattttcct
    11581 ctaacttagc aatagtctct tcttctgcat ctggcattaa ttgaactata aatcctccag
    11641 caaactctac ttcatcttct tttgtaagaa cacctaaagc tactactgat ggagtctgct
    11701 ctgatacagc aaaataataa gtaaagtctt ctgcaatttc tccacttacc aatggataag
    11761 tcccattata tggttctcta agacctaagt cttttataac ttttacaaca ccttctgtac
    11821 ctactgctgc tgctacattt aactttccat ttggatagtc ttcaacctct acttgtggat
    11881 ttcctacata tccttttacc attcccttag agtctgatgt ggcgataata gttccaattg
    11941 gacctccacc ttttattatt acagtcaatt tatcactatc atttttcatc attaatccca
    12001 ttatagatgt ggctgttaat gttcttccaa gtgctgctgt tgctactttt gtagtttcat
    12061 gtaatcttct agcttcttct actgtatttc tagtagttgc gacaaatgct ctaacttgac
    12121 catttcctga tgtcgctctt agtacataat ctctcattaa tattcctcca tgtcccgtta
    12181 attgtcttat ttttattata ttactacctt aatttatcta taaatactta tctatcttaa
    12241 ccttgggcaa aacccatttt attttaattt tactaagtaa tagttactat attttaattt
    12301 ttatctatag aactttataa ttattaaaaa ataaaagtca taaagtatca tcactttact
    12361 tttaatctat aaaactgcat tttctatatt tttatatcat ggttattttt aataattatc
    12421 ttatatagtc aagaatatct tattttttac atacaaagaa gattctttga ctttcttcac
    12481 taggtttgtc aaatgtaaaa tccccataat attttatatc cttaaatcca gatttttcaa
    12541 gcatatcaac tatctcatct tcagtatatg ctctttgttg gtgtatctct tcaaatcttt
    12601 caaactttcc acattcatct ttaataaaga atgtaagttc catttcaatt agattctctt
    12661 cttcatcaaa gtaattttgc cacatatacg ctatatcttc tctattttct ccatacatgt
    12721 tatttcccaa tatgttggct aatttataaa aagaacttat atcaaatatg aatattccat
    12781 ctttctttaa taattcatat gtctttgaaa atacattttc caaatcatca tcataagtta
    12841 tataattaaa tccatcacaa gcacaaagta cacagtccaa atcagatatt tcaaaatcaa
    12901 gctctgatat atcttgttga agtaaaacta attctactcc ttctttttct gccttttctc
    12961 ttgctacact aagcatctca tcagatatat ctataccagc tatatcgtaa ttttttttag
    13021 ccaatggaat tgtaagatta cccgtaccac atgccaattc caatatattt tttactttta
    13081 caccttcact atctattata tcttctatat atttaaccca accattataa tcaacttcat
    13141 tcataagttc atcatataca aacgcaaaat cactatattg actcatcctt tttattctgc
    13201 tcctccacaa tctttttaag cctctttttg cgtgatgtaa gcatacctcc aattcttcct
    13261 gcttccttag ctgtaagtcc tgcccatcca cgttcaccaa ctttatccat aagacctagt
    13321 tctgaggcta tctcatattt cattatatca tcatttgtca atattttagt attcttgcca
    13381 ttagttatct tcttttctct ttgtactttt tctttcaaaa aaatcacccc ttcttatttg
    13441 tatttttgtc aattaaaata aatttataca aatatgtaag gggtatttat taatacactt
    13501 tttttaaatt taggtattta tgctttttaa tctttataat ttattttcct aatctattgc
    13561 ttctttgtat tttaataatt ttaaataaac attcaatgca tttacacata tcttttcatc
    13621 aaaattaaag tttatattat gaagaccatt tacaaatcct ttttcttcgt ttcttgaacc
    13681 caacataaaa aataatcctg gaacttcttt ttgataataa gaaaaatctt ctgatatcat
    13741 aagtggctct aactctacaa cagtatcact tccaatagcc tctacaaact cttgatacaa
    13801 atccttgtca tttttaacag cgatataatc atctataata tttatattaa ccttacagtt
    13861 ataagccaat tcaaaccctc tagcaagttc tctaactcta agcttcattt tctcatacac
    13921 ttctgggctg aaacatctca tagtaccttc catctttata ttttcagcta ttatatttct
    13981 tctagctccc ccttcaattc tgccaatagt caacactgca ttgtctattg ggcttatatt
    14041 tctagacact atagtttgaa gtgagcttac aaagtttgaa gctatcacta taccatcaat
    14101 accattttgt ggcatagccc catgtccact tttagacact atttctatgt caatatctcc
    14161 tatttgagcc ataaaatagc cctctcttgt acctacataa ccttctggta actctggata
    14221 tatatgaagc ccatatattt catcaatatt gtattttcta agcactcctt catctattaa
    14281 agcttttgct cccccaggac cttcttctgc cggttggaat ataaatacta tattatcatt
    14341 taagtcttct ttattatcat ttaaatactc aattaatcca agtaagatag tagcatgtcc
    14401 atcatgacca cataaatgtt ttaccccttc ttcactatga agaccatcta tgtcagctct
    14461 aaaagcaata tcttttttag gtcttttacc ttctattaca cctactatac ctgtttctaa
    14521 ataaacttca tactcaattc ctaatttatc aagataatct cttatatatt ttgaagtctt
    14581 atactctttt ctcccaatct ctgcaatagg atttaatgac ttcctatgtt tataaagttg
    14641 ttcttgtaaa tatgatgcct ctctcatgta atctcctcca atttctaatt tattaaaaaa
    14701 tatttctata tttctttata attaaaatat tatatagcat gttatactaa ttaaaaataa
    14761 aaagctaaat tgaatataaa aatattttgc attattaaat aattcaccat taataataat
    14821 tcccctgttt cataaatcaa aacagaggaa ttattattgg aaaattttat ttttagcaag
    14881 agaagaaatg tttagcaagt ccacccttag ctgtttcttt gtacttatcc atcatatcta
    14941 taccagtttc tttcattgtt tcaacaactt ggtctaagga gacagaatgt gagccatcag
    15001 ttaataatgc ataattagct gcatcataag ctctttgagc tgccattgca tttctttcta
    15061 tacatggaat ttgaacatat ccatatacag ggtcacaagt cattcctaaa tgatgttcaa
    15121 gtgctatttc tgccgcatat tcaatatggt ctatagttcc accttttaag tatgctactg
    15181 ctgctgctgc catagaacaa gcggctccaa cttcaccttg acaccctacc tctgctccag
    15241 atattgaagc gttagttttt attatattac ctattattcc agctacaagt agtgcttcaa
    15301 ttattttttc gtcatcataa ttatagaaat cctgcattgc aaagaatatt cctggtatta
    15361 ctccagcaga accacaagtt ggtgctgtaa ctattatatt accagaagcg ttttgctctg
    15421 aagctgctag tgcatatgca tatacaagtg ttgagaagct cttatctttt ttatatgcat
    15481 tataaaatgt acttgctctt ctttttaata ataacttgcc tggtattatt tcatctgtac
    15541 ttagtccatc atcaatagat tttctcatag catcttttat agtttttata taatctttta
    15601 tgtcttttgg ttcacattcc aacacaaaat ctaccaaagt cttattattt tctttgcacc
    15661 attttactat ctcatccata gtatctaagt gatatatact attgctttta ctgtttcttt
    15721 gaccttcttc tgctatagtt cctccaccta cagaatatac agtccattca gctgttacat
    15781 ttttatcttt atctaaagct tcaaacttca tcccatttgg gtgaaaatct tttataatat
    15841 cttcttccca aacaatttcg acttttttag gtgctatagt tttttcaatt atatagtcag
    15901 ttaagtgacc tttccctgta gctgccaaac taccatataa tattgctcta aaactttctg
    15961 catctgggtt ttcattctta aatctctctg ctgctctttg tggtcccata gtatgagaac
    16021 ttgaaggtcc acttcctatt ttaaaaagtt cctttaaagt atccatatta ttcctcctaa
    16081 atttacggta ttattgctta ttgttagatt ttacgcacat atttattata acctaaatat
    16141 attaattatt agttattaaa ttctcataaa atttattttt tatttccatt ttggtatatt
    16201 ttattaaatt aatatgcata ttttgagaat aaaacattta tttaaaattt ttctaatatt
    16261 aatataatat aaatatctta tttaaacagt taagatatta actataccta ttaaaattaa
    16321 ttaatttgta ctataattat tattgtactt aaaaaattaa atttagataa aaaaggagat
    16381 ttgattatat gttatttaaa ggttctgctg ttgctttagt taccccattt acagaggata
    16441 ataatgtcaa ctttgaaaaa ttaggtgaat taatcgaata tcacattgag aatggcacag
    16501 atgcactagt tgtttgtgga acaacaggtg aggcgactac tatgagtgaa tctgaaattt
    16561 ttgctgtaat aaaatataca gttgaaaaag ttaataagag aatacctgtt atagcaggta
    16621 ctggttcaaa caatactatg ttgtctgttc atatgagtca agaagctgaa aaattagggg
    16681 tggatggtct tttaataata actccttact ataataagac taatgagaaa ggtcttaaac
    16741 ttcactttga aacaatagca aatagcgtta agttgccaat tattttatac aacgtaccag
    16801 gaagaactaa agtgaatata aagccatctg ttgtggccga acttgctaaa atagataata
    16861 tagttgctgt gaaggaagca agtggagact tagctcaagt tgcagaaata gctaaattag
    16921 tacctaaaga ctttgctata tactccggta atgatgatac tatactacca ctattatcat
    16981 taggtggaag tggtgttatc tctgtacttg ctaacatatg tcctaaagaa acacatgatt
    17041 tagtaactaa attctttgaa ggtgatattg aaggctcaaa aaaattacaa cttgatatgg
    17101 atgctttaat tgctgcctta tttatagaag taaatccagt tcctgttaaa actgccatga
    17161 acattttagg ttttaatgtt ggtgatttaa gacttcctct agctgaaatg gaagaaacta
    17221 atcttaatgt gttaaaacaa gaattgacaa attttggatt taaattttag caatatttat
    17281 ataaataagt tgattttttc ttaaatatgt attattatta gaataatcaa attttaatat
    17341 cctattaacc tagggtagag gtagctgtaa ctaagagtat ccaatagtag ctggcaggtg
    17401 ttaatgattg gaaaaaggat ttacagccga agaaaatatt tcttgcaata atattttctg
    17461 ggattgcaca taacatgtgt aatactgtca cttatttttg taggtgatga gctacagggt
    17521 ttactttgag tgataaatta gacgtacaaa gtataccttt gtacgttttt tgtatataat
    17581 taaagttgtt aatataatat taaagttttt ggatatataa aaatattaac atatcttact
    17641 tgtataacta aaataaaata atgttttaat aatttaaaat tggggtgaat ttatatgaaa
    17701 aaagtaaatg tagcaatagt tggtgctact ggaatggtag gtagaacatt tttaaaagta
    17761 ttagaagaaa gaaattttcc aatagaaaat ctatttttat tctcttcttc taaatcagct
    17821 ggttctaaag taatgttttg tggtaaagaa tatatagtag aagaattaaa ggaaacttca
    17881 tttactgata gaggcataca aatagcatta ttctctgctg gtggaagtat tagtgaaaag
    17941 tatgctccaa tagctgcttc aaatggaatc ttagtagtag ataactctag tcaatggaga
    18001 atgaatccag atgtgccttt agtagttcct gaggttaatc cagaagccat aaaaaatcat
    18061 aagggtataa tagctaatcc taactgctct actatacaag ctatggttcc ccttaaagct
    18121 cttgatgata aatacaaaat taagagagtt gtttactcta cttaccaagc tgtatctggt
    18181 tctggtgtta aaggtgttga agatttagag aaaggaatga acggtaatcc tactggattc
    18241 taccctcatc aaatctctta caactgtctt cctcatatag attcattcac tgaaaatgga
    18301 tacactaaag aagaaatgaa aatggtaaat gaaactatga aaatacttga taactacgat
    18361 ttaaaaataa ctgcaactac tgtaagagtt ccagtaaaaa atggtcatag tgaatctata
    18421 aatgttgagt ttaacaatcc atttgagtta gatgatttag taaaaacact tgaagaagct
    18481 ccaggtgttg tagtagttga taatcctgct aaaaacgaat atccaactgc tgtagaattt
    18541 gatgatagag atgaagtatt tgttggaaga ataagaagag attttagtgt tgataatggt
    18601 gtaaatcttt gggttgttgc tgataatata agaaaaggtg ctgcaactaa cgcaattcaa
    18661 atagctgaaa tggctttatc atacgattta gtataatata caaacttgta taaatatata
    18721 attttgtaaa atattaatag taactcaaaa acaagcatta gaagtaactg taatataaaa
    18781 cacaaaaata aaaaagaggg gattgatttt atatgatatt taaaggttct gctgttgctc
    18841 tagttacacc atttacaaag gataacaaag ttgattttga caaattaggt gaattagttg
    18901 aataccaaat agctaatggt actgatgcta tagtatcttg tggtactact ggtgaagcta
    18961 atactatgac tgatgaagaa caacttgcta ctattaagta tgttgttgaa aaagttaata
    19021 aaagagttcc tgttatagct ggaagtggtt ctaatgatac tatgcactca gttaacttaa
    19081 gtcaagaagc tgaaaaatta ggagtagatg ctctattaat aataacacct tcttacaata
    19141 aagctaataa agctggactt aagcgtcatt ttgaaacaat agctaacagt gttaaacttc
    19201 caattatact ttacaatgtc cctggaagaa cttgtgtaaa tataagtcct agccttatag
    19261 ttgaacttgc taaaattgat aacatagttg ctgtaaaaga agctagtgga gacctaggac
    19321 aggttgcaga aatagctagt ttagttccag atgactttgc tatctactct ggtaatgatg
    19381 atactatact tcctttatta tctttaggag gtcaaggagt tatatcagta cttgctaatg
    19441 tttgccctca agaaactcac gatttggtag ctaaattctt tgaaggcgat attgaaggtt
    19501 ctagaaaatt acaacttggt atggatgctc taatcgcagc tttatttata gaagtaaatc
    19561 ctataccagt taagactgca atgaatttat tgggattcaa tgttggagat ttaagattac
    19621 ctctagcaga aatggaccca gcaaatttag aagtgttaaa gaaagaatta gttaactttg
    19681 gactaaaagt taatgcatag taaagattaa tgcatggtaa aaagtaagtt tggtcaaaag
    19741 ttagccctta atcataggag gaaattatgt taaaagttat agcaactggt tatgatggaa
    19801 aaatgggtaa gattttagcc gatactataa gagaagataa tgaattagaa ttggtttgtg
    19861 ttgcagctag aggtctggat agctatgaag gcgaccttaa aatttatgaa gatatgagta
    19921 ctatagttga ggaagctgat gtagttatag atttttctca tcactctaat ttagataata
    19981 tcttatctta cgttttaaag actaaaacac ctttagtaat tgctactact ggatataatg
    20041 atgacgaaat gaacaaaata cgtgaagcgg ctaaagttat tcctgtattc caatcatata
    20101 atatgtctct tggtgtaaat gtattagtaa aattggttaa agatgcagct aaattactag
    20161 aaggatttga tatagaaata atagaaaaac atcacaacag aaaagtagat gccccaagtg
    20221 gcacagctgt tatgattgct aacgctataa aagaggtttt gccaaacgtt gaaaataatt
    20281 atggtagata tggtcgtagt gcaaaaagac aaccaaatga agttggtatt catgctataa
    20341 gaggtggaaa tattgttggc gaacatgatg ctctatttgc tggagacaat gaggttctta
    20401 caataagcca tcaagctcaa tctcgtggta tctttgcaag tggttctata gctgctgcca
    20461 aatacttagt taagcaaaaa ccaggatttt ataatatgga cagcatgttg ggataatagt
    20521 ataaatataa taatacacaa tataaattaa acatatacaa tatatatata ataatatgca
    20581 atacaaatca aacatatata ataaatttat ttaatactat gtctatacaa taaaaactaa
    20641 ctaaaataaa atataaataa taagaagtaa gtatatacta aaattgacaa ataagtatat
    20701 aataaaagaa atataatgaa atacaaggag aattaacaaa tggtaaattt aaacgatgct
    20761 tatgaaatag caaggtatat aaaagaagtt aaaaagaaaa cacctgttaa ggtatatgta
    20821 aatactaact ctttacactt ccaatcaact gataaattta aggtatttgg aaataatggt
    20881 tcttatatat ttgtaggtga ctatgatgat ataatggaag tattagataa acatggaaat
    20941 gatataacag atactcatgt agaatatgat agaagaaatt ctgctattcc tctaaaaaat
    21001 actttaagtg aacatgcaag aattgagcca ggtgcaataa taagagacat ggttactata
    21061 gaaaaaaatg ctgttgtaat gatgggagct gttataaata taggagctgt tattggtgaa
    21121 ggttctatgg tagatatgaa tgctgttatt ggagctagag gaactcttgg taaaaatgtt
    21181 cacttaggtg ctggtgctgt tgttgctggt gttttagagc caccttctgc tactcctgtt
    21241 atagttgaag atgatgtttt aataggagct aatgcagtta ttcttgaagg tgttagaatc
    21301 ggtaaaggtg ctgttgttgc agctggtgct gttgtaacta ctgatgtaga agctggtgct
    21361 gttgttgctg gttctcctgc taaagttata aaaatgaaag atgaaaaaac ggctgacaaa
    21421 actaagctta tggaagacct tagaaattta gactaattaa atagtactat actattaatt
    21481 aaacaataat atactaattt aaaaaattaa ttaaaaaggt gtctctgtgt gaatttaatt
    21541 caccagaggc accctctttt ttacaatatt tttatctttt atttataatc attttatttt
    21601 tttacaattc tcttattcat tcttatatca tttttatttc ttgctgacaa gtatttttat
    21661 ttccttcaat ttttcaatat atcaatgtaa taaactatta aactttttat aatttaataa
    21721 agtgaaaacc attaatcttt ctataattta ataaaatgaa ctactaaatt ttctatatat
    21781 caatagaata aattattaaa ctttttataa ttcaataaag cgaaccatta atctttctat
    21841 aatttaataa aatgaactac taaattttct atatatcaat aaaataaact attaaacttt
    21901 ttataattta ataaatagaa ctgctgttta ctactggttg tgcatcttta ttgccacaaa
    21961 gcacaactaa cagattactt accatagctg ctttacgttc ttcgtccaaa gtcacaacat
    22021 tattttcact taaattagtc aatgccatct ctaccatact tacagccccc tcaacaatct
    22081 ttttacgtgc tgctattata gcctctgctt gttgacgttg taacattgct gctgcaatct
    22141 ctggtgcata agaaagatga gtaattctca cttcacacac ttcaattcct gcaatgtcaa
    22201 cccttgattg taattcatct tttaatctat ctgctatttc ctgactacta ccacgaagag
    22261 atttttcatc tccatcttca gatacatcat atggataaag tcttgaaaca ttgcgaatag
    22321 tagaatcaca ctgaatagat aagaatgtat tataattatc cacattaaat actgcctttg
    22381 ttgcatctat aaccttccaa attacaacca ctcctattat aattggattt cccaataaat
    22441 cattgacttt ctgcttttca ttattaagag tcattgtttt taaagatact tttttgcccc
    22501 ttgaattagt tgatatatct accttttcat cagatgaaga tttcgataca ttaattcttg
    22561 atactgctgg gtttattgct gaacaaaatg gattcaccca atagtaccct tcttttttta
    22621 tagttccata atagttacca aacaaaacta gaactatagc ttctttagga ttaatcattc
    22681 ttagcccaaa aaataggata aaatctacta ctataaatac aagtcccaaa attaacataa
    22741 gtgaacctat tccagtaaat ccactatcaa gttttactat cccaaaaacc atgataccta
    22801 ttgaaactac taaaaataat aaaatcatta tcaaagctgc cactccattg aatacttttg
    22861 cgtctttctc ttgataagtt ttttcttctt ttaacatatc tatcactccc atctcagtat
    22921 attaagttaa gatatctcta atttaataat aacactttta acaattaaat tgttgaattt
    22981 aaaattaata ctatatgttt atatattttt tatacatctt tatgcataca tgtatttagt
    23041 tattttaatg tattaatatt gatttccaaa taaatcatat gatatatgaa tatggtattt
    23101 caaaattaac tttttagttc atggggcact ctttttggta tgaagtcaaa tatattttta
    23161 tcatttaaac aatgaaaaag aaatttatct atattttgaa aaatccactt aaatttttta
    23221 atgtaagata aattgtaagc ttattatcac taatattatt ttatataaat tatattagtg
    23281 ataaaataaa cttatagaaa tataacaaat ttttattaat ttcataggag gaatttatgt
    23341 tgaaagttat aattagtggc tataatggaa caatgggaag agttttagca gaatgtgtaa
    23401 aaaaagataa tgaattagag ttggtttgtg gtgtagctag gtctgctgat gaaaaaggtc
    23461 atcatagtga tacaaagctt tacaaaacaa tgactgatgt aactgaaaaa actgatgtca
    23521 taatagactt ttcacatcat gagactttag agccaatatt gtcatatgct ctaaaaaata
    23581 atacacctgt agtattagct actactggat atgacgaaaa tgacttaaat aaaatagaag
    23641 aagcatcaaa aaacattcct atattattat catcaaatac atctcttgga gtaaatatat
    23701 taataaaatt agttaaggat gcttcaaaga tgttagaagg ttttgatatt gaaatcatag
    23761 aaaaacatca caatagaaaa gaagatgccc ctagtggaac tgctactatg atagctaatg
    23821 caattagaga agtcttgcct gaaagtgaaa atacacatgg tagatatggt agagattgta
    23881 aaagaaaacc aaatgaaatt ggaatacact ctataagagg tggcaatata atcagcgacc
    23941 atgatgttat atttgcaggt gataatgaag ttcttacatt aagccatcat tctcaatctg
    24001 atgaagtatt tgcaaacggt tctatagttg cagctaaatt tttaattggt aagcaatctg
    24061 gactttataa aatgtcagat atactaggat aagttttgta cataataaat ctcaatctat
    24121 ttaggtattc atattcatca atttgaatat ctaattgatt gagatttcta ttctaaaagt
    24181 ttataaattt tatgcttacc ttttaaatat aatcattttt cttaaagatt atcttattta
    24241 ttggtatatg gaattatatt gtttattatt ttataatctc ttcatcatat aaacctatta
    24301 aatattatcc tatttattgg tatatggaat catattgttt attattttat aatcttttca
    24361 tcatataaac ctattaaata ttatcctaat ttattggtat atgaaatcat attgtttatt
    24421 attttatagt cttttcatca tataaaccta ttaaatatta tcctatttat tggtatatga
    24481 aatcatattg tttattattt tatagtcttt tcatcatata aacctattaa atattattct
    24541 atttattggt atgtggaatt atattgttta ttattttata gtcttttcat catataaact
    24601 attgtattct ataaactgat acatatccag ctgcagaagt acctgtcgtg cctgaaccaa
    24661 atgtaaaact taaagtaaca gctgctgctc tagcatcaac taaaaagctt aaacttacac
    24721 ttacatctga tgaagtaagt gtatttactg cttttgcaat aaagttattt tgagctactc
    24781 cattaaagtt agtagtaatt gacataaatc ctgcaagtga aagtgtggca cgtataccga
    24841 aagatacatt gtatatccct ccaactgcaa gatttatagt actacttgtt ggaaaagtta
    24901 tattagttcc atttataaat gatgaattaa atgtcactaa tgtattattc actaaacttg
    24961 aagaggacac tgtaaattgt gcattgtttg ctgctaacac tcctgttgct cctgtcgccc
    25021 ctgttggacc tgttgctcca tttgctccgg ttgctccggt cgcccctgtt gctcctgtcg
    25081 ctccatttgc tccggttggg cctgttggtc ctatcgctcc tgctactcca tttgctcccg
    25141 ttgctcctac tgctccggtt gggcctgttg ctccatttgc acctgttgct cctgtcgctc
    25201 ctgttgctcc tgctactcca tttgctccgg ttggaccggt tggacctatt ggacctgcta
    25261 ctccattcgc tccggttgct cctactgctc ccgttgggcc tgttgctcca tttacacctg
    25321 ttgctcctgt cgctcctgtt ggacctactg ctccatctgc tcccgttggg cctgttgctc
    25381 ctactgctcc ggttggacct attgccccag ctactcctgt tgctcctgtc gctcctgttg
    25441 gacctaccaa accatttgct ccggttgctc ccgttgggcc tgttgggcct accaaaccat
    25501 ttgctccggt tgctcccgtt gggcctgttg ggcctgttgg acctaccaag ccatctggac
    25561 ctgttgctcc tactgctcct gttggaccta cctctccatc tgctcctgtt ggacctgttg
    25621 ctcctattgg gcctgttgcc ccagttgttc ctgttgctcc agtcgctcct atacttcctg
    25681 ttggacctgt tgctcctgta tttcctgttg ggcctgtagc acctgttact ccatttgctc
    25741 cggttgctcc tgtatttcct gttggacctg ttattccatt tgctccggtt gctccggttg
    25801 ctccagttag tccggttgga cctgttgctc ctgttgctcc tgtattccct gttggtcctg
    25861 ttattccatt tgctccagtc gctcctgtat ttcctgttgg tcctgtagag cctgttattc
    25921 catttgctcc ggttgctcct ttgtttcctg ttggtcctgt tattccattt gctccggttg
    25981 ctcctgtatt ccctgtcgga cctgttattc cattcgctcc agtcgctcct gtatttcctg
    26041 ttggtcctgt agaacctgtt gttccatttg gtccagtcgc tcccatattt cctgttggac
    26101 ccgttattcc attcgctcca gttgctcccg tatttcctgt tggtcctgtt attccatttg
    26161 ctccggtcgc tccggttgca cctctaggcc ccgtcggtcc tgttggacca gtagcacctg
    26221 ttggccctgt tggacctaca tcttgatata aacttggacc tgaaatatca ctcatataat
    26281 taatcctcct tttttaaagt tagagtaata ctctataaat attgagtatt accctatata
    26341 attatatgaa tcgacaattt cttatgtgat aatgtatcta tttgcttata tcaacatata
    26401 tgctttgatt cttgaatttc attacttttt tgtaaaatct tttacaatta ttaatacaat
    26461 taaaaaactc atttcccatc aattatttaa aattttttta ttatgttata aaaaatagat
    26521 aattaaattg cataaaatta atttaatact actttaatta ctttttaaat aaaaaaacct
    26581 caacacgatt aataaaaatc atattaaggc atccacaaaa ttatataatt gcttatctat
    26641 cttcatcagt aacattgaat atataaggaa catttctata ataatcacca aaatcaaacc
    26701 catatccaac cacaaactta tcttctattt caaatccaca ataatctggc actaaatcga
    26761 cttttcttct gcttggttta tccaaaagaa cacagcattt taaactagca ggatttttag
    26821 cttttaaatg ttttaacaca aaatccatag taagtgcaga atcagtaata tcgtctacaa
    26881 ccaaaacatc atatcctgct atatcagtag tcacatctga aactacttta acctttccag
    26941 tactttcttc attgtttcca taactagatg tagtcataaa attaactcta agtggtaagt
    27001 ttatatttct aactaagtct gcacaaaata taaagctacc ttttaataga gaaactacca
    27061 taagattttt atctttataa tctttttcta taattgctcc taattccctt aatttatctt
    27121 taattttttc ttctgaatac aatacttccc atttttttgt ttctatatcc ataaaaagac
    27181 ctccaattgt aataatcctt cttaatttta taagagattg ctcacttttt caatactctt
    27241 attcaatatt agtcaatatt attgagtttt tatgttcatt caacaattat taacaatgta
    27301 tcttatattt taagtagttc atatatccac actaaattac atactatata ttttagacaa
    27361 aaaaagaaaa aacttgcact aacaagtttt ttctattata cataaattta tacataatca
    27421 tattttatta gagggtacta attaaattta tttatgctat ttttgttatc cctcataata
    27481 caaaacatat tataaagtac tagtcaactt tattcatatc attcattaca tttaagatta
    27541 tctcatctgt tatagacata cctttgtcac ttactctacc taagttttgt atactttgct
    27601 caacacttga accaactata ccatttagtg gtggtacaaa acaatcttgc ttagctatta
    27661 tagcactttg tatagctgca gatgctgcag aagataattt taatgcacaa ccagctttcg
    27721 caccgtcaca tatcattcca cttaagtcag ctatcatatt ttctatagct ccttctattt
    27781 gcttttcagt accattcata agccaagaga taccagcagt tgctccagta gaagcagcaa
    27841 caccacatcc acatacagca gataatcttc ctgtatagtt cttaatataa ccagttacta
    27901 gatgagatat agctaaagct tttgctagtc tttcatcaga ttgtgggaat tttttattat
    27961 aagcaacgat tggaagaatt gctgttaatc catgatttcc acttccattt gaactcataa
    28021 caggcatttt tacccctgcc attcttgcat ccgaagctcc agcagttaac atcattgcat
    28081 aatttataac atcattacct aaaagacctt cttctataga ctttttaatt ccatatccga
    28141 caccaatacc agttttttgt tttaatccat attcagccat ctcttcattc atttttactc
    28201 catctagtaa aaattctata tctttaaaat caattccttc tacatttttt attaattctt
    28261 gtatagttac tgtatccatt aaactttcag ctttttctgc cacatcattt gaagctgata
    28321 cttttggttc attgtctaat aatacttctc catctttttc taagaacgta aaattatcat
    28381 gtttagttct tattctcact ttagcaatat gattttttcc ttttaacaca acttctataa
    28441 atactttctc tcttgtatca gcaggtgtta ttgatagtgg agtattatcc atataatcct
    28501 cagctatttt aacttcctct tttgttagag tttctaacac acttaatcca ttttcagaat
    28561 gtccacctac tatacctaga gctgctgcaa tttttaatcc taatctttca gtacctggta
    28621 tacctacgca cataccattt ttatagatac taggactaac taacatacga ttttcaacta
    28681 tttcttctcc taataattcc tttgcctttg cacatgctag agctaaggct actggttcag
    28741 tacaccctac tgctggttta acttctgcct ttaacatttc cactaattct tttcttatat
    28801 ctctcattat atataaacct cccaattaag tttagattta gtactcaaat aattaattaa
    28861 ataatttctt atctatattt atagcaaaat acatgccata ttttttatat ctttttatat
    28921 ttttagtctt atattctata accattggta tgactgcttc tatttatgta tttttttatt
    28981 tacatagagt atttatattc tttaaaatgg ataaacatat gacaatttcg caaaaagaca
    29041 aaaaaaagtc tcaaaatgag attctatatc attttgagac ggctcaaatg tattattttt
    29101 taacttataa aaaccatttt ttaattcata aaaaaagatt ctgcaaaaca cttatagtat
    29161 aagtttaaag ctttctttaa agttatgaat ttagaaaaat ttaaatttta tatagctttt
    29221 tattatatta ctatgaaatt atattgtatt tttcaatttt tctatataat gtagctcttg
    29281 atattccaag tgctttagct gttaattctt tatctttttt ataatctttg tacttattta
    29341 aagcttttat tatctcaatc ttctctagtt catcaagtgg aattatccta tcgcttttaa
    29401 cttctaactc ttctacagag gtattcttaa ttttttgagg aattacattc attgtgatta
    29461 ttgaagaaga agacatattc acactatatt caataatatt ctgcaactct cttatattcc
    29521 ctggccactt atattccatc atagcatcaa caaccctgct atctatgtca ataacatttt
    29581 tatctaattt ttttgaatac tctttaacca tatgcttgat tataagtggg atatcttctt
    29641 ttctctctct taaacttggt aattttatag gtataacatt taatctatag tataaatctt
    29701 ctctaaaagt gccattttga accatttttt ctaaatcttt atttgtagca gcaataattc
    29761 ttacatctat tgaaacattt gatttacttc caattttatt aagttccttt tcttgtaata
    29821 ctctcaataa ctttgactgt aaatgcaaac tcatatctcc tatttcatct aaaaatatac
    29881 ttcccttatg agctacttca aacattccaa gctttccacc ttttttagct ccagtaaaag
    29941 ctccctcttc atatccaaat aattcactct ctaataaatt atccggtata gcagcacagt
    30001 ttacagctat aaaaggatta tctactctat tgctatggtt gtgtatggct cttgcaaata
    30061 gctccttacc cgttccactt tccccagtaa taagtactgt tgatgttgat ttagaagcta
    30121 ttagggcttc ctttttagaa tttcttatag ctacactttc tccaattatg ttttccaatt
    30181 ttattctata atctttattc atcttattgt agttttttat agcatctctt ttatctataa
    30241 aatcaataac atatcccttt aactcatttt ttaatactat tttattgata ttgtatattc
    30301 ccttcgaatc atatccatat cttttataaa aaaaactaca ggatttgtcc ttcttaaagt
    30361 tatttataga aggtttcttt ataaaatcta attcgacaaa tatgtttttg ccagttatac
    30421 tatcttttat attgaaaagc tctttaaatt ttgaattata tttatcaact tttccatcca
    30481 catcaataga cacaacagct ttgtccatat tgtttaatag tgtctctaat tttttctttt
    30541 ctaattcaag ctcataagtt ttaatttgtg cttttaattt attagatatt aggtctgcca
    30601 ttttgcctaa aaagtttata agaccacttt tattattttc tataatttgt gcttgttctt
    30661 ttgtaaaggc tataaggcct ataacaccat gagaatcgcc atcacatata ataggacaac
    30721 acacctctgc aaactctaca caagccccac ttttatagca ttccctacat atatcactac
    30781 tctttgggtc atcaattagt attgtaatct gttctgtaag tgatttttta aaagccgtat
    30841 atttatctac tttctcacct attttgtcta tatatactcc tgtacctgca atcctaaata
    30901 actccttatt tactatagtt acatctatat tgagtactga tactatagct tcagctatat
    30961 tttgaatatc agattttata tattctaaac ctatcataat catcattcct tatttttctt
    31021 taacataagt atttgtctta tttctttaag aatcaagtat atcttattta aagtaaattg
    31081 gattgacatc aggattacaa agagcataac aattattatt aactcataat ttatttccat
    31141 ctaaacatct ccttataaca aaagactagg cttaacctag tcttttatat tatatccata
    31201 tttatatttt aactcattta tcttcttttt cccacataaa tatattttaa tttatttgac
    31261 tctttaatca taccatcaaa gtttaatctt gaaaatatat taaatacttc atgtggatag
    31321 tgaatctttg caacagaacc acaaaattta tgtttaagat agtttaactt aacttttaaa
    31381 ttctttaaat ttaaatcctc taatataacc tctccattgc cctttaacac tctttgtatt
    31441 tcagatgaag ctttttcaca atctgtaata acattaaatt ggtcaactat tattatcttg
    31501 ttgaactgat tatcatcaaa tggtagaaac tctaacttac ctttaactac attgcaattt
    31561 tcattataca ctagagaata acagacatct tcataattac tattctccag tatagttaca
    31621 gaagttccaa gacctctcaa tctcttgcct aggtttccta tatgaccaac aagcaatatt
    31681 ttatcaccta cattatactc aattgtatcc aaaaaacatt ctaattcatc agatgtatat
    31741 ttagctttaa ccggctgttt atttatctga cataatatgc ttttcacaat gcttcacctc
    31801 ttcatttcct caattataaa ttatgtacag attacattat aataaatggc gagcaactat
    31861 tcaagagata tttcattagt atttttttaa acaatcttta tacattttaa tatgatatta
    31921 taccaaattt attaggattt tattcataaa aatcagtcta tttttcatgt ttattcattg
    31981 ttattttcat ccaatttttt tattttcgat atggatactt catatgccat ttttttgacc
    32041 acattatttt catctatttt tttctcatat tctctacttt gaaccctacc ccaaagctgt
    32101 attctatcac caacctttag attctttgca aatttagcat ttctacccca aactattgat
    32161 ggtatataat cagatttatt ataaggtctg tttatagcaa ctaataaatc ggttatttcc
    32221 cttcctaaag gtgtcttcct atacacaggt tccttacata catatccatc taaaaatata
    32281 ctattagggt ctttattttc ctcgtctatg ctttttattt ctctgacaaa cactgtaagc
    32341 actaatctat ttttattatt taatgttttg ttatatgatc ttaattggcc tacgacattg
    32401 actaaatttt gtttatcaag gtcaatatct tggagaagtc tttctgatac agtcattgga
    32461 agtatatcaa aagaatcgct tagtctgttt atttttattt tcatattata aaatttttct
    32521 ccaaatatct catggctaaa ttctagctta ttgtctaact cccctcttaa gtttactacg
    32581 ttattctcat ctttaacagc tatcatattt tatccctcca caattgttaa ttcttatatt
    32641 tctggtatca gcttatataa taaatatatt tataagggat gtattttatt acaaatcttt
    32701 gattgctgtt taaaagtttt tatttagtta taaatttcat ttaatatttc acttttataa
    32761 ttataaaatt attatctttt tgtaccacat atactaatta aactacctat ttcagtttta
    32821 tattaattat aaaaatccta ttatatagtt aataagtcat atataactta aatttattca
    32881 ctctatataa gcaacttcaa cgaattaaat ttattcaaat aattcagatg aatattgagt
    32941 cattataaac ttatttttct taattataag ttatattaga cttattacag gttatatttt
    33001 tattaatctg tattaatata tgaacattta ttgaacttat tgattataaa atttaaatat
    33061 ttagctttaa attatgcttt attctttaaa aatacaacgt tttttacatt aaatctacta
    33121 aatcactata tatattacat aattggcatg ttaattgctt tatattaatt tagtaaataa
    33181 aaaactgccc taaaatatta attcaatatt ttaaagcagt ttataatctt tattattagc
    33241 tgtaatattt aataaacact aagtagtctt taactaagca gtctttttgt ttttatttgc
    33301 agccattatc attgtagcag aagtataagc tataactact attactaatg cttttaatat
    33361 atccattggt agagttttta ctatatatgc agctacgaaa actcctatta aaccacatat
    33421 agctattata actgatgctt ttttagtata tgcatcttct tttatgaatt tcatacttgc
    33481 aactggcatt aataatgcac aagaacccat aactattggg aatgcaactt ttgcagacat
    33541 tcctaagaaa taaaccattg ccatacctgg agcatataaa cctataccag ctgtcattaa
    33601 agctcctagt ataaagttac ctattacacc tattactaat ttaacaccag taagacctaa
    33661 ttcatttcct ccacctggta atacgttaat ccaaggatga cctaataaca ttaatattgc
    33721 tgttaatgct aaagttacac ccataacaag ttgtattttt ctctcgtcca tcttagctat
    33781 tattcctgca cctatatatg aaccaactgc tgctgctaca accatagata ctaaagtaac
    33841 accatcaact ggtatgattg atgtaaatat gaatgcttct gtaacaactg gaatagtatg
    33901 tgctacgttt aatgtaccag gcattttttt atctggtata tttaacttta aagcctttga
    33961 cattgctatt gttggagcaa atgaccctat tcctaatgta tcaaagaagt ctgttataaa
    34021 tcctgttaca gcaacttttg gagtactttg ttcttctagt ttcccctctt tgtttgcttt
    34081 agctacgtct ttaccaaaca caactgcaaa gattgctgcg aatactacat ataatgcctt
    34141 taagattgct accataatcc atcctcctat ttttttattt tttcatatat tctgaatctt
    34201 atagcctaaa agctatagaa tagcaaccga caagtggcta ctattctatt ttaatttcct
    34261 aatttattta ttacttattt aagaataaat ccatgtttaa gtgggtcttc ttcatctatt
    34321 acaaaatgat taaatccagt tatataagca gaaccagtta ttttaggtac tacagcattg
    34381 aaatctgcaa ctttagtttc ttcaactatt tcacctttga ataaagttcc taatatactt
    34441 tcatatacga atttttctcc tacttttaat tcacctttag catgtaaagt agctaattta
    34501 gcacttgttc cagttccaca tggagatctg tcaacttgac cttgaccaaa tataactaca
    34561 tttttgtaag tagcttctgg atgagttggc tcatcatata tttcaactaa gtctacagtt
    34621 tttatatgag ctaaagttgg atgttgtatt tctattttct catttattat atctctaagt
    34681 ttcatagcta gttcagttaa tttaccagca ttttgaggtt cgatttttaa acctaattga
    34741 cttgcatgta ttatagcaaa gaagcttcca ccaaatgata tgtcaaattt aacagttcca
    34801 acacctggta agtcaacttc aacaccttct ttatataaga aagctggtac gtttaagaat
    34861 gatacttctt tagcttttcc atctacaact gtaacgtctc ctcttattat tcctgctgga
    34921 gcttccataa ctacatgagt tacaggctct actgctggaa ctacacctgt ttctatagct
    34981 gctgtcatag ctcctattgt accatggcca cacatgttaa ggtatccgcc accatccatg
    35041 aagattattc caaagtcagc atctgggcaa caaggttgag tcattacaga accaaacata
    35101 tcattatgtc ctcttggctc taacattata gcagttctta aataatctag attttcctct
    35161 aaatattctt ttttctcagg catagaattt ccttttatgt taggtattcc acctacaact
    35221 attctagttg cttcacctgc tgtatgagag tctatagctt gtatacttct gctaaatttc
    35281 atatcaaatt cccccttagg tttattttgc gtctttacgc atttttaatt ttaggaaagc
    35341 caatgctttt ttaacatcaa cttcatccat gtgattgtct ccaactactt ctgtttctat
    35401 gtagcctgtt gtctttttat aatcaattat ggtagcttta tccaaatatg gattggtaat
    35461 tactaatcca tctgctccca taatagtaag acctactgta ggtatatctg attttacaaa
    35521 ctctgctata gcatttgtaa aatcaatatg ttggtttcct gccatatcta aagttataat
    35581 cactccatca ggtcgcaatg attcaatcca tcttccaagt ctagttgcta aaaatgtttt
    35641 ccattcattg tgctctgatg tcccaactac aaccactcct aacaagtcta tgtcagaatc
    35701 attatctatc aaatctagta acgggtctct aaatatatga agtgatgtgt ttttcattga
    35761 tgtagaaatt cccacaaaac tcattccctt acttaaattt tttatccatg ttttctcatt
    35821 ttaagtttta aaaatgccaa agctttttta gcatctattt catttgtgtt gttttctcca
    35881 actacttctg tctcaatacc ttcttttgat ttgttcatat caactatggt atccatatat
    35941 tgattgctta caacaaactt agcttgagtc ccattaaatg taactccaac tacaggtata
    36001 tctctttttc ctatttcctc aaatgtgtta gcgtagtcaa catgtgagtt tccccaacca
    36061 tctgatgaaa ctatagctcc atctactctc atggcttcca gccaagcggc cgttctttgt
    36121 cccacaaagg ttttattttc atttccatct ggagttccta ctactattac tccaagtaaa
    36181 tctacatcct tatcactttc aactatgtct aaaagtgggt ctctaaagtg atgaagagat
    36241 gtttcttttg ttgaaggtcc tattcccata atatccacct ctcttaagtc atagctctaa
    36301 gggcaccatc tctgtattca ttaggagata taatcatagg tacatttccc atatctataa
    36361 ttgatgttcc tccttcaaat ccactaggtt cctttgagaa taactgattg tcatacatag
    36421 caccttgacc tgctatttgt tttactatga caaccttttt tgcatttggt cgaattttgt
    36481 caaaatattc acgaacttga gtagcatttc ttccgtcttc cattttcaaa gacgctctta
    36541 tttcttgtat aaaatcatca catgctctga atgctgcaag tggaagtgtt ctttcaaaag
    36601 gtaaacctcc ctttaaagtt acatctacat ggattatata atcttcaact gatggagttc
    36661 catatctccc aaataccatt tggtctttca aaattccttc tgaagaacca aattcatgca
    36721 tttgatttcc atcttcatct gcaccagtta acattacata tacacccgtt aaagtatgtg
    36781 taataccctc tcccagtctg cctaaaactt ttgttgatat tggtataatg tccataatag
    36841 tattaatttc tctgtcataa tctccaggtt ttataatgtc tacttttatg tctgttatta
    36901 actcttctct ttcttttatt tcatctatac ttgataaatc tattgtaagt acatctttct
    36961 ttatagaagt ttttgaaccg aaattaactt cattcatatg aaacggtttt ataacaagtc
    37021 ttctcattat tttttcttcc ataatatcca cctttgttct taaaacccct ctcctaaccg
    37081 tgtaggagaa gggttctata ttttcaaata taaattacat tttaaacgtg agctttatat
    37141 tcgtatggta atggaactat cttaccagca gatggtattg caactaattg ctctaatgta
    37201 tctcttaata tatgtgtttg catttctata ttatgtggtt caccagcatt agcacccatt
    37261 ggaactaatg gagcaactgc tctaggagtt ccgttttgtc taactactgg aggaagagct
    37321 gctattatta ttgtaggtat tccagcttcc tctattgctc tctgcacgat cacggcagtt
    37381 ctatggcaag tacctcagcc agctgtcata actacaccgt ctactccctc ttccttaagt
    37441 ttagcagcga tagcaggacc agtttcttca gtgaagacat gttggtttcc tccaccaccc
    37501 atgaatgcat agtgcatagg agctactcct ttgataaatc cttctgcagc taattcatgt
    37561 aatctatcta tagggaacat acagtttata tctttgttta catctccatt gtcatatcca
    37621 ccatgtgata ccatcatttc atcaactgta gctgtattag gtatagctct aaatgtagta
    37681 tctcctgcta gattaaatct cttgtcagat tttaaatgaa caccagctgc tgtagctaaa
    37741 gctatagtca tatcttttaa ttctttagtt acaggagtcc aaactggagg aggtgttatt
    37801 ggaacgaata tttcagattg aagtccttgt actgttgtaa ggctcatata tttcctctcc
    37861 cttctatttt caaacgatta tttattattt ttcttcttgt tttgattgca tttctgcttg
    37921 tttttctttt tctctcctaa tattttctac atactcttga ttaggctctg gacttaatac
    37981 ctcaggatag cttagcatga atccaacttc ttgacctatc ttaagttcgt agtcagttat
    38041 aagcattcta ttaggtattt taaattgtcc aagtctacct tttaggtcta tagtcactcc
    38101 accacctttg atgtcaacta taataccttc ataaaaagtc tctgatgaga tatattttaa
    38161 cctatccatt ataattctac ctctctatac tgttttaaac ttggatctaa atttatgtta
    38221 ttgtctaaat cttagttagt atctaaattt tagttttcgt ctttttcgta tatttctctt
    38281 ctcttcttac tcattggtag agattgctca ttctcttcta atactatttt ttctcctgta
    38341 actttttcta tagcttctat gttatttaat ttaacatttg gattccattt tctttcagga
    38401 gctttaactt ctacaccttc tatagcattt ttaagcatag ccattattct aacagcatct
    38461 tctggagcta aagtgttgtt agataatatt tcattctcta taccttgctt agacttattg
    38521 ttgtctacca tgtgagtcat gtatttatta ccaacaacta gagcaccttg aacagctgag
    38581 aaacttacac caactactgg tatacctctc atacctattt gctctatgtg agatgcgaaa
    38641 tctatatggt tgtttccgaa accttcagta gttactacag ctccatcaac ttccatagct
    38701 tcaactaaca tacctaatct cttagataca tagaatttct cagcatttac ttgtggagaa
    38761 ccaacaaata caacacctac taaatctatt tcttcgtctt ccatagctct tattactaat
    38821 ggctctctcc agtaatgtct tgacatttct tttgatgcag gtcctataca agttaatgca
    38881 tggataccac catctaatac ttcaagtgga gataatacaa ctggcatgtt tcctaagtca
    38941 acgtttggtt gagctcctaa tgtaccaact ggctcaactg gtaatattag gttatcatgc
    39001 attgcacctt gtcccattat ttcttttata acaacaactt tctttttacc ttctcttctg
    39061 tattgctcta ttacttcttc atcaactact aaagagttgt caactttctt taatgcttct
    39121 cttatttctt gagttacata gtcaaatgca cggtgagcag ctaaaggtcc tggtctttcc
    39181 atgttagttc ctgcttttat tgttacttga cctttgatga atatttcacc tttgtcagca
    39241 gcacctggtc taccccacat tatagtagta tttaactcac cttctgaaga accaaattca
    39301 cctatttgaa ctccatcttc atctgtacca gttaatacca ttacagctcc atctataact
    39361 ctagttatac ctgaacctaa ttcgccttct tctttagtag cgattggttg tatatctaat
    39421 acagcttcac tgtaagtttc atatttatca ggagttatta tttctaactt catatctaca
    39481 actaactctt gagtttcatt agcttcttta catatttctt ctgcgtttct gatgtataaa
    39541 gtagttcctt ctattttagt ttcatcagca aattcaactt tatctatttt aaagtgtttt
    39601 ttagttaagc ttcttaaaag tttcttttct tcaacttttg gagctacttc tgctgctgca
    39661 cttgccactg gagcagcaac gtttgctact ggagcagcaa ctcctgcttg accagctact
    39721 gataaaggta tctctaagct tatgttctta ccttcaccta tgtgtatttt aactgtttca
    39781 cctgcaactc ctgtgcttac tggaactact gctgcttctg atactggagc tgcttcttct
    39841 actgtttctt ctactacttc ttctttagcc tctcctgctt tgtatccttc tacgttatct
    39901 gtagtcattg gagaaagtgc atctaaagtt tcttttaact tagctcctag tacttgacct
    39961 atagttaatc cattttctgg tattgttaat aatccagaat cctctaagtc tgcaaatatt
    40021 gctggatctt ctaagttttc tggcgctata attgttcccg cttcaaatct acaacaacaa
    40081 actgctgggt catttgcatg ggcttgagct gtttctaaag ttattgacat agtataaaat
    40141 acctccttac attattttct tatttattat tttaaatctc aattcctcaa caggtaaccc
    40201 cttaactgag aaattaagta aattaaaacc tacataattt gttatgatta atattatttt
    40261 tttctcactt aaagtcactt tatattataa gcaattccta tgccaactta ttaacaaaaa
    40321 gattttttct ataatagatt cttcatctat cgctattttt agccaaatat tatataaata
    40381 tttatttaaa atatgttatt ttttgtattt gtaaacattt tctcaaacaa aaaaagtgtc
    40441 aattaatcaa cactttttca ttttttttaa cacatctata tacacaacac ttatacaagc
    40501 gttggcttta ctaggtttta aattaaagtt tatcattttg tcaaaattta gtcatgtgtc
    40561 atattttaga cacatctatt ttgtattggt ctatcttata atacaaagtt gttctgggta
    40621 tatttaatat cttagctgct ttagctttat ttccattaga cattttaagt gccttggtta
    40681 tattctttat ttcaattttt tgagttgcct tcgttaaatc taaaggatac tcttcttctt
    40741 cagtactatt atttgtagaa tccatcatat aacttggtac atcatccaca tctattttag
    40801 aattttgtga caatacaaca atattttcaa tcgtattttt taattctcta atattacctt
    40861 tccatctata attctgaaat atatctatta catctttgct aattgtaaga acaggcttat
    40921 tattttgttt acatatctcc tctaaaaagc tatgaaccaa taatcctatg tcttcttttc
    40981 tctctctaag tggaggaatc tttatttcaa caacatttaa tctatagtat aaatcttctc
    41041 taaatttttc tgcctttacc attttactca aatctttatt ggtagctgat attattctag
    41101 gatttatttt tattgtggta tctccaccaa ctcttcttat ttctttttcc tgtaatactc
    41161 taagcaactt tgcttgcata ctaagaggta aatcggcaat ttcatctaaa aatacagttc
    41221 catcttttgc aagttcaaat atacctattc gacccttttt acttgctcct gtaaacgcac
    41281 ctgattcata cccaaaaaac tcactttcaa ataactcatt tggtatggca ctacagttaa
    41341 caggtataaa ttgacctttt ctctcactat aatcatgaat agctcttgca aatacttctt
    41401 tacctgtacc actttcgccc caaataaata tgctactatt tgttctagcc acttgttttg
    41461 caatcgcttt cgatttttcc aatttataac ttttacctaa aatcttatca aaactacctt
    41521 tagataaatt tttgacttcg ttctttagat attttaatgt atcatttact ttttcaagtt
    41581 caagagctaa tatcttagct tctgtaacat caacttctgt acatacaaca ccgataaatt
    41641 cttctttata aaatatagga tttgcatgaa ctaatgcata catattttct tttttatctg
    41701 taaaatacaa gtcactcatt ccaactttac tatttagtac tttttctgaa atagtatttt
    41761 ccaaaaattc attcatcggt cgaccaacta tatcttcaga ctttatatca tacctttcct
    41821 ccataaactt attccacaag ataacaacac cttcgttatt tatagcacaa attccttctt
    41881 taatttgacc tataatatac ttcaaagtta atccataatc ttctaaatgc atatatagat
    41941 agtccctaat gtgctcttgt tttagaactc caataatttt attatctcta agaacaggaa
    42001 gaatccctat attttttaat atcattatat ctctacattc atctaaggtt aaaccttcgt
    42061 tgacatatat tacatccttc ttcattataa gttttactgg ttgaccttca tactttttat
    42121 gcatctcata aagattatgt atatccgtca tggatataat gccttttaat tggtcagatg
    42181 aatctattac cattagagtt tttgtgttgc tttttatcat ttcttttatt gcatcttcaa
    42241 ttcttgtgtc ttcgtctata gttgtaaatt ttgtatccat tacctcttct acttttttta
    42301 cttctggtat cgaaaacata aagtcccccc tttatagtag tacctcatca aaacttattt
    42361 atatatattt attctagcat attataataa ttatttatta aataattatt attaaaattt
    42421 attaagtaat tagcatttaa atttatataa tcactttaaa atttataatt aaat
//
LOCUS       DAGSWI010000020        40867 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.020, whole genome shotgun sequence.
ACCESSION   DAGSWI010000020 DAGSWI010000000
VERSION     DAGSWI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.020"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            203..1039
                     /locus_tag="MJF80_000966"
     CDS             203..1039
                     /locus_tag="MJF80_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBZ0279639.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1392..2969
                     /locus_tag="MJF80_000967"
     CDS             1392..2969
                     /locus_tag="MJF80_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBZ0279640.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEYTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2962..4146
                     /locus_tag="MJF80_000968"
     CDS             2962..4146
                     /locus_tag="MJF80_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBZ0279641.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGKEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     NL"
     gene            4164..4763
                     /locus_tag="MJF80_000969"
     CDS             4164..4763
                     /locus_tag="MJF80_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBZ0279642.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4787..5257
                     /locus_tag="MJF80_000970"
     CDS             4787..5257
                     /locus_tag="MJF80_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBZ0279643.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            5629..6375
                     /locus_tag="MJF80_000971"
     CDS             5629..6375
                     /locus_tag="MJF80_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBZ0279644.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHVELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            6436..7110
                     /locus_tag="MJF80_000972"
     CDS             6436..7110
                     /locus_tag="MJF80_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBZ0279645.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKREFIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
     gene            7479..8825
                     /locus_tag="MJF80_000973"
     CDS             7479..8825
                     /locus_tag="MJF80_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBZ0279646.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMYYSQMTARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEYIDAKVLAGESMYYYKKIKD"
     gene            8852..10246
                     /locus_tag="MJF80_000974"
     CDS             8852..10246
                     /locus_tag="MJF80_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBZ0279647.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSSKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GESGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDIKMHKPEVGDIISTIIKDKSILSAHYGGTWEKIGEKI
                     EFGITVHYYKLIEK"
     gene            10456..11892
                     /locus_tag="MJF80_000975"
     CDS             10456..11892
                     /locus_tag="MJF80_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HBZ0279648.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNEEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKDMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGKETVYYYKHHEPEKTNQIQ"
     gene            12236..13180
                     /locus_tag="MJF80_000976"
     CDS             12236..13180
                     /locus_tag="MJF80_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBZ0279649.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRF
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            13349..14281
                     /locus_tag="MJF80_000977"
     CDS             13349..14281
                     /locus_tag="MJF80_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBZ0279650.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            14386..14853
                     /locus_tag="MJF80_000978"
     CDS             14386..14853
                     /locus_tag="MJF80_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBZ0279651.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(15097..15951)
                     /locus_tag="MJF80_000979"
     CDS             complement(15097..15951)
                     /locus_tag="MJF80_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar uptake protein"
                     /protein_id="HBZ0279652.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSFKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSYIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VLMLSIIK"
     gene            16117..18081
                     /locus_tag="MJF80_000980"
     CDS             16117..18081
                     /locus_tag="MJF80_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBZ0279653.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSANPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAINHITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            18081..18542
                     /locus_tag="MJF80_000981"
     CDS             18081..18542
                     /locus_tag="MJF80_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0279654.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            18545..18865
                     /locus_tag="MJF80_000982"
     CDS             18545..18865
                     /locus_tag="MJF80_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBZ0279655.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            18881..19981
                     /locus_tag="MJF80_000983"
     CDS             18881..19981
                     /locus_tag="MJF80_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBZ0279656.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            20101..22701
                     /locus_tag="MJF80_000984"
     CDS             20101..22701
                     /locus_tag="MJF80_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBZ0279657.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDENIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            23170..23505
                     /locus_tag="MJF80_000985"
     CDS             23170..23505
                     /locus_tag="MJF80_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0279658.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            23642..24919
                     /locus_tag="MJF80_000986"
     CDS             23642..24919
                     /locus_tag="MJF80_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBZ0279659.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            25040..26428
                     /locus_tag="MJF80_000987"
     CDS             25040..26428
                     /locus_tag="MJF80_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBZ0279660.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            26799..27512
                     /locus_tag="MJF80_000988"
     CDS             26799..27512
                     /locus_tag="MJF80_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBZ0279661.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            27630..28499
                     /locus_tag="MJF80_000989"
     CDS             27630..28499
                     /locus_tag="MJF80_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBZ0279662.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANINLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKELVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(28654..29799)
                     /locus_tag="MJF80_000990"
     CDS             complement(28654..29799)
                     /locus_tag="MJF80_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBZ0279663.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(30132..30908)
                     /locus_tag="MJF80_000991"
     CDS             complement(30132..30908)
                     /locus_tag="MJF80_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBZ0279664.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            31490..32464
                     /locus_tag="MJF80_000992"
     CDS             31490..32464
                     /locus_tag="MJF80_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBZ0279665.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            32562..32867
                     /locus_tag="MJF80_000993"
     CDS             32562..32867
                     /locus_tag="MJF80_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBZ0279666.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            33023..34183
                     /locus_tag="MJF80_000994"
     CDS             33023..34183
                     /locus_tag="MJF80_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBZ0279667.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            34233..35135
                     /locus_tag="MJF80_000995"
     CDS             34233..35135
                     /locus_tag="MJF80_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBZ0279668.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            35215..36108
                     /locus_tag="MJF80_000996"
     CDS             35215..36108
                     /locus_tag="MJF80_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBZ0279669.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      36161..36342
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            36570..37700
                     /locus_tag="MJF80_000997"
     CDS             36570..37700
                     /locus_tag="MJF80_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBZ0279670.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            37772..38797
                     /locus_tag="MJF80_000998"
     CDS             37772..38797
                     /locus_tag="MJF80_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBZ0279671.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            38800..39996
                     /locus_tag="MJF80_000999"
     CDS             38800..39996
                     /locus_tag="MJF80_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279672.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(40124..40849)
                     /locus_tag="MJF80_001000"
     CDS             complement(40124..40849)
                     /locus_tag="MJF80_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBZ0279673.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT        15890 a         4345 c         7173 g        13459 t
ORIGIN      
        1 aagaaataat taaatatctt tcaatgaaaa tatgataaaa tatgacaaat taattgatga
       61 caataaaaaa tattttgtta ttgtagtatt atagggtaaa ttgcaaaata tatacgaaag
      121 taaaatttta aatattgaca ttaaattaaa tatactatac tatgtaagtg tgatgagaga
      181 atttttttag aggggattta tagtgaatat aaaaaagata tttaataaca atgtagtagt
      241 aagctcccta gaagatggca cagaaataat tgtcactggt gctggagtag gatttaagaa
      301 aaaagtaggc gatttgatag atgaaaattt gatttctaag aaatattttg ttcaagacga
      361 tcaaagggac aaatacaatc aaatacttaa taagacctct attgaatact ttaaaatttc
      421 agaagaaatt atagaaaagg ctaatgaagt tttaaatact caagtgaatg attcaataat
      481 attagcctta actagtcaca tagaatttgc tgtccaaaga gaaaaacaag gtataaaatt
      541 accaaactta atattgaatg agacaaaaca actttatagg gaagaatttg aatttggatt
      601 atgggcaatt gatgaaattg aaaaaaggat aggaataaaa ttgcctgaag atgaagcagg
      661 atatattgct attcacataa tcaatggttt ggaaaattct caaaaagatg aaggtattaa
      721 tatactagag ttttcaaaac aagttattca gataattgaa gaagtacatg gatttaaatt
      781 ggatgtaaat tcattaaatt atactagact tataactcac ttaaagtttt ttgttcaaag
      841 gattctccga aaagaaactt acaatgatag tgatattgaa gatatgtata agttagtgaa
      901 taaaaattac aataaaccta aagtgtgtac tgaagaaatt gcagcattgg tattagacag
      961 atttgaatat aagattagta aagaagaaga attatattta atgattcaca taaataaaat
     1021 aacaaacagt ggttgttagg attgtaactg ataaagcagg caaaacctaa gtcattgagc
     1081 ttaactatta gattaagttt tttgatttag gtttttttat tagatacaat caaaatacag
     1141 acagtgactt ttaggattgt aactggttaa gcaggcaaaa cctaaatcat tgaatttaac
     1201 tattaggtta agttttttga tttaggtttt tttattagat acaatcaaat acagacagtg
     1261 acttttagga ttgtaactgg ttgagcaggc aaaacctaaa tcattaaact taattattaa
     1321 attaagtttt ttgatttagg tttttctata aatttgatta tgtttttaga taaaaatagg
     1381 aggggaaaat tatgaaaaaa aataagttag gtttatggaa cttcttccaa atgttaggaa
     1441 aaacgtttat gttcccaata gcactactta gtgtttctgg gatgatgcta ggacttgggg
     1501 caggttttac agaccctaag atgattgaaa tggttccatt tttagggaat gaatatgtta
     1561 acttagtttt aaacttcatg ttaacaatag gattatttgc atttaacaac ttgggagcat
     1621 tatttgcaat ggcaattcca ctaggattat taaaaaaaga aaaagagttt ggtgcttttt
     1681 caggattagt aggatttata gcaatgcaca taggtacaaa tttttattta acaagagctg
     1741 atttgttagt accagcagaa cagatgtcaa ctaatggtca agctatgatt atgggtatac
     1801 aaacatataa tacaagtgtt ttaggtggta ttgtagcagg attaattgtt tatgcaatat
     1861 atgataaagt tagtaattta aagataccag aatcattagg attctacagt ggaccaagat
     1921 tagttccaat tgttagttta atagtcatga gtatagttgg attattaata cctattgttt
     1981 ggccaccatt tttcaatgca ttccaaagtt taggaagatg gatttcttca gcaggaccat
     2041 taggatattt ttcatatgca gtggcagaaa gagttacaat tccatttgga ctaaatcatc
     2101 ttgtaacatc cgtattccgt tttacaccaa taggtggaac tgctgttatt gatggagaaa
     2161 cgtactttgg tacattgaac atgtttatgg catatgtaga aaataatcag attatacctc
     2221 ttgatttagc tggtaaaatg gaacaaggta aattgatgat tcaatatgga ttagctggtg
     2281 ctgcattggc tatctaccgt acagcaaaac cagaaaatag aaaggcactt aaaggacttt
     2341 taatatcagg agtgttgaca gttattatag gtggtataag tgaaccaata gagtttctat
     2401 tcttattcat atcaccagca ttatttgctt tccatacatt tatgaatggt ctagcaaaca
     2461 tggtacttcc atacttaggc gtgttgatgg gattcacagg agatttaatt gcatttataa
     2521 gttttggtgt gttaagagga acagctacag gatggccaat agcagtatta gtagcagttt
     2581 tatattttgc aatatactat tttgtattta agtgggcaat tattaagttt aatataaaga
     2641 ccccaggacg tgaggataaa gaaataaaag tatctgaagg aaatggttca gatttcaaaa
     2701 atttgtctac atataaagga caacaaatga tatctgcttt aggtggtcaa tctaatatat
     2761 tatcactaga taattgtgta actagattgc gtttaaaact acaagatgta tcattaataa
     2821 atgaggatgc aattaaagag gctggtggta tagcagttgt taaattagat gagtatacaa
     2881 tacaagtaat tataggaact caagtgtatt ctttaagaaa gcagatggat aaagctatgg
     2941 aaggtcattt ggaggcatgt aatgattaat tttgataaaa aagtagaccg attgggtaca
     3001 tattgtaccc agtgggacta tgtagaagat agatttggta agaaggattt gcttcctttt
     3061 acaatttcag atatggattt agagtttcca tttgaaataa cagaggtgtt gcaggaaagg
     3121 ttaaatcata gagttttggg atacagtaga tggaaacatg atgattttag aaattcagtt
     3181 acgaattggt atattaaaag atttaattgt agcgttgata gtaactggat atattatagt
     3241 ccaagtgtaa tgtattcaat tagcaaactt attgagatgt taagtaatga aggagatgga
     3301 atccttataa atactcctgc atacaatgct ttttatgatg tgataacaaa taacaatcgt
     3361 aatattataa agtcacatct tgtaaatagg aatggattct attcaattga ttttaatgat
     3421 tttgagaaaa agtgtaaaaa atctaagata tttattttat gtaatcctca caatccaact
     3481 ggtagagtat ggagtgagtt agaacttgtt aagatgatag gaatttgtaa gaataatgat
     3541 gtcaagataa tatcagatga tatacacatg gatattgtat atgacaatag aatgacacct
     3601 atcttaaatg ttgcaaagga ctatttagat tctgtattca tttgtacatc agcgtctaaa
     3661 tctttcaata taccagcatt gactgggtca tatgtgatta taccaaatga aggtattaga
     3721 aataaatttg aaaatatcac tagataccgt gattttgtaa attcgccagc aattctagca
     3781 atattagcta caatggtctg ttatgatgac tgcgaatatt ggttagatga actattaaaa
     3841 taccttaaag agaatcttaa atatacgatt gattatataa aagataattt agaaccattg
     3901 aaattggtca tgccagaagg ctgttacttt gcttggatag atttttccga acttaaaata
     3961 agtggtgaga aattccaaaa attattgatg gatattggag aagttgctat tatgtcgggg
     4021 tctgtatatg gtaaagaagg tgagttttat ttaaggttaa atgtggcatg ttcaagagaa
     4081 aaacttgaag atggtttaaa taggattaaa aaaactatgg attatattgc aaaaagaaat
     4141 ttatagtagg aagggagata gttatgggat atttaaaaac aatggttgat gttatgacta
     4201 ctgaaatgaa tgccataaga tgtttaatag atgaggcagg aatagaatat gaaagtatag
     4261 ttagtgaaat tgcaaattgt gaaggcaaag taatttttat gggtgtagga aaatctgcac
     4321 atataggtaa gaaattggca gctacatttg ctagtactgg tacacctagc ttttttgtac
     4381 atgcaacaga ggcagtgcat ggagatttag gaatgattga aagtaaggat attacaattt
     4441 taatatcaaa tagtggaaat tctatggaag tggtaaactg tataaaatat attaaagcaa
     4501 taggttctaa gacaattgca tttacttcaa acagaaattc agtactggca aaagagtgtg
     4561 attatgcttt aatatatcct gcaaaggatg aagcagacca tttaaatcta gcaccaacaa
     4621 catcatctac aattactttg gttttaggag attctattgc atgtgcatta tctaagagta
     4681 gtaattttgg ttcttcagat ttttataagt accatccagg aggaagttta ggtgagaagt
     4741 taaaaacagc taacaatgga taaaagcaaa aaggagagta gtttttatgt tcaaaatgtt
     4801 taaaaaaaaa tcaataaaat cacccatatc aggaaaagta atagagttat ctaaaataca
     4861 agatggtgta ttttctcaaa aaattatggg agagggtgta gctatagatt ctacaggaga
     4921 cattgtgtat gctccaatca atggaaagat agtagtagta gcagagacta aacatgcatt
     4981 tgtaatagaa ttagaaaatg ggatggaact tcttattcat gttggtttag atactgtaaa
     5041 tttaaaaggc gaaggttttg aggcattggt gagtatggga gaacaagtga aggaaggaac
     5101 accaatgttg aaaattaacc gtcctttgat tgaatcaaag ggtatatcac ttattactcc
     5161 agttacaata actaatcatt cagagtacaa tatgaataca tgtaatgtag gcaatagtgt
     5221 tgaaggtggg aaagatactg taattgagtt taaataaaga actaaaatac aaggttaata
     5281 tttataaata cctataaact tatgctttat aagattgatt gtatagattt gttgtataga
     5341 tttgttgtat aaaaaagaac tctttaatta taaattaagg agttcttttt tattttgtaa
     5401 atattgttta gatttatata gaaaaacact tagtaataaa tagaataaat aagtatttat
     5461 atagctaaaa atctaaatag ttttgtttaa atatattttt atattttgta atttcaaatt
     5521 aataaataat tagatttgat aaacaaaaaa taaaaatgtt gattacatta ataaacttta
     5581 atataatata tttatgaaaa atgtaaactg tgaaatggag gtagtaacat gaaaattgaa
     5641 aatctttcta ttgatgatat taaacgaggc tatatattta atgatgattt aaattgttat
     5701 agttgcatta aatgtacaaa aacatatgag gtaggagaaa tatataaaat aggtaataga
     5761 ttctttgaag cttcaaaagc tattgaactt catgtggaat tagaacatag tgattattta
     5821 gagcaattgc ttaatagtga ttcaaagtac aacacattga ctgaaaatca aaaacaatta
     5881 tttcaattat ttattagtga tttaccagat aaggagatag caaaacaggt tggagtatcc
     5941 acatctacta tacgccatca aaaatttatt ttcagagaaa aagcaaaaca ggctaagttc
     6001 tatttagcac tatatgaatc tgtatttgaa gataaatcta ataaaagtag tactataata
     6061 ccaatccatg aacatgctcg aatggtagat gaaagatatg tagtaactga agaagagaga
     6121 aaacacattt tggatacatc atttgaaagt atgaatcctt taaaattaaa agtattttct
     6181 tcaaaagaaa agaaaaaggt cgtaatttta tctaagattt tggaacaatt tgaacgtggg
     6241 aagaaatatt cagaaaagga agttaatcag attttagaat ctatatatga tgattttgca
     6301 acaattagaa gatatctaat tgagtatggt tttatgagta gaaataaaga atgtacaaag
     6361 tattggttaa cataattaaa ataataaatt ttactaatgt gtaatgttat ttttatctag
     6421 ggggaggagt caaatatgaa tgatgaatta aaatctattt tatacaataa aggtgtagac
     6481 attgttcgct ttgttgatat atctgaattt ccaataaatc aaacacaagg tttttctaaa
     6541 gcaattttat tttgtatagg gttatcaaaa aaatttatta cagatatata caataactta
     6601 cctacagata gtgatgagtt tttggaaaaa gaggagaaag tagaagaatt agctgactgg
     6661 atttcaaaat atattcaaaa taaaggatat agagcatatt ctcaatcgga gaaaaacaat
     6721 ctagaacatg gatattttga aaaagcctat attaatccag aaatgcaatc agggattagt
     6781 cctttaccac ataagacaat agcaaatatt agtggcattg gatttatggg aaaaaataat
     6841 ttgtttgtaa cagaggaata tggatgtgct tttagcatgt gtacagtact tacagatgct
     6901 ccaatttctg ttgaaagata tcctttgatt gattcaaaat gtatggattg taatgtttgt
     6961 gtagaaaact gccctgcaaa agctatacat ggaaatgagt ggactttgcc aggaaaaaga
     7021 gagtttatta ttgatgtttc aaagtgtttt tgtgtattga agtgtatgat gagttgccca
     7081 tggtcattaa gatatgcaaa tcaaaaataa ttttactaga gaaggtatta aattgaatat
     7141 tataaaattt ttaaaatgta agattatata agatttttca aaataaaaat ttgtatagaa
     7201 cactcagaaa tgagtgtttt tttattggga ttaaattata ttgatacaaa gcttaactat
     7261 tttaatcatt atataaaatt tgtattaaaa aattaagaat aacttggtaa tagtgaaaat
     7321 ataatgagat tataattgat ttataatata actataatga tattgtaaaa atgaaaattg
     7381 tcctctcaaa attcccaaag agagtaaaag tcacttcagt tatagtcaaa aaaatttttc
     7441 tgtgaaaaga atttagattt gaaaaggagg aattaatcat ggaaattgaa actaaaactc
     7501 cagattggat taatgtacta gaattaaaca gtaaaataga tattactggg aaatatgatg
     7561 taagtaatat gatacaaaat attgtctctg ataagtcact agaaggttcc attatatatt
     7621 ttccaaaagg taattactta tttgagaaag gtattaaaat tagtcaacaa ataacacttc
     7681 aaggagattc ttattatgga ggaggtaatc aagtatcaaa tttagataaa aaacaatcaa
     7741 tcataggaac aaccaatttt ataacccgag gagtttctaa tatgagtatt attacattgt
     7801 caggaacaag tcaatgtatc aaaaatatta atttttatta tgatagtcat gatatagaga
     7861 aaataccgcc taaaaatgta tctgctatta cagaatatgg agaaacacaa ggactatctc
     7921 attttgaaca tctgtttatt tctggatttt cagggatagg aattgaaatt ccatactatt
     7981 caactggtaa tgatattact gttagttcat gtggtcttgg tatgaggctt ggagagaagt
     8041 caatgctttc tagtagcaag atttacgaat gtaaaaatgg aatggagatt actactggtg
     8101 ttagcttaaa taatgtgaga atagaagaaa taaaagaaat aggtattaat aataaaggat
     8161 ttggttttaa tctgattatg aatcttatgg tcgaccaatg tggatattgt ggatttatgt
     8221 ttgaaaaaat gtactatagt cagatgactg ccagaattac tagatgcggt cagtattata
     8281 aaagtgttga ttataatgct tatgagaata tggaagatag aaaagaagaa gcttattcca
     8341 ttttttatgg agatatttta gaaaattgca atattgtatt gatgaatagt aatattgata
     8401 ccttggaaaa tgattcactt aatgaacata agatatatgt aatcaaggca aatgaaacaa
     8461 acaatgtaat gctaacatgt tatgctgaag cagataactt tatacaatgt gcaaaaggta
     8521 acttgctgtt agaaaatggt gaaaatactt ataaatttta taatggtgag atttgttctg
     8581 taggaggtgt tcaaatttct gatatagatg aaaaagattt aatcaaaata atcgagaaca
     8641 ctctgtatat agatgataaa ggtaagtatt taatggttcc tagattaaaa aagaatactg
     8701 taatatattc agtactagat tctacagaag aaataaataa attacatggt ggaatatggg
     8761 aatatattga tgcaaaagta ttagctgggg aatccatgta ctattataaa aagataaaag
     8821 attaaattat caaactagga ggtagtgatt aatggaagag tctataataa ttaaagatat
     8881 aagtggctgg attaatgttc ttgattatgg aatttgtaat gatgaaacaa aagatgtaag
     8941 ttccaagttg caagagatta tggatgctgt accagaagat tctattatat attttccaaa
     9001 aggaaaatac ttatttgaaa gtggtgttaa gattaagaaa cgattaactt tatgtggaga
     9061 ttcttatatt ttggcgaatg ttcctaaaaa tactgctgga gtaacacaat ttacttttaa
     9121 aggaaagaaa aatcctaaaa gtgaaaatat agcaattata acagcaaaaa gtgtaagaca
     9181 ctgtattaag agcatttgct tttattcaga tagttgtgat agagatatga catctaatca
     9241 tgaacctcca acagatggaa aacctagatt tcatcataat gtaattataa attatagaaa
     9301 tatatcggcc attgtatgtg aagataaaca gggagaatca ggtcattatg aaaatctatc
     9361 ttttagtggt ttttctggaa ttgcacttga tatgcctaat aattctacag caaatgatat
     9421 tacaattttt acatccggac ttggaattag tacaggagaa aattcaacta ttgctaacag
     9481 taaggtttgg ggctgtgttg atggaatgat aatttctact ggaactttta taaatggtat
     9541 gagggtagaa gaaataaaaa aagttggaat taaaaatatt ggtagaggtt taaattttat
     9601 tacaaatatc acaattgacc aatgtggata ttgtggagtt tggtttgatt ctatatccaa
     9661 tgttcagata tcaggaaata tcacaaggtg tgggcagtac tattatgatg taagctatga
     9721 taattattta atgttggata atagagttaa agaagcatat tctttattct atggtaattt
     9781 attggaatca agtaatatag ttttatcaaa taccaataaa gataattggg aagatttcaa
     9841 agaagataag cataaggtgt atcttgtaga agcacttgaa accaagaatg tactattaac
     9901 atgcaatata gatgctactg actggattgt taagtcagaa aaaggaaatt taacgtacaa
     9961 taatatgcga caaactgtta tattttacga tggaaaacta agtagtatca atggaattgg
    10021 tatatctgat gaagattatg gggataaagt caagataaaa aaaggaaata tatatataaa
    10081 tagtaaggat attaagatgc ataaaccaga ggtaggtgac attatctcaa caattattaa
    10141 agataaaagt attctttctg ctcattatgg gggaacatgg gagaaaattg gtgaaaaaat
    10201 agagtttgga ataactgtac actattataa attgatagag aaataagtaa atacgtagta
    10261 tttgaataga tgtattttat tggagagcta ggactatagt tttaatgtaa attgtttggt
    10321 aatagcatgg attgaataat ataaaaataa tataaaaaat taatttgatt atgtaaattt
    10381 ctatgaagta gtaaaactgt gactttagtc ataggaggtt caaactaagt aaaagggaaa
    10441 ggaatatata atattatgga aggaagaact gggattaaag atggaagatg gattaatgta
    10501 gtagaaagag gtattaaaaa tgatggttcg tacaacgtag gtcctgcttt gcaggatatt
    10561 attgatgaaa tagaaacaga tttttgtgtc ttatattttc caaaaggtaa atacctattt
    10621 acaagtggtg ttaaaattaa taaagaaatt gtattgcaag gtgattctaa ttcatctaca
    10681 ggtactacac aatttataac tcgtggagta gaaaatatga gtattattac attgacaggg
    10741 aagaaacaat gtattaaaaa tataaatttc tattctgata gttgtgagat acaggtcaat
    10801 gaagaaccac cgacaaaagg taatcctaaa tatcattatg agatgattat caataagaat
    10861 gaaaagaatg ttgagatgaa caatgtatca gcaatcctgt atgataacag taaggatatg
    10921 attaaaggtc taggtcatta tgaaaatatt tatataagtg gtttttcggg tacaggaatt
    10981 aggataccat attactcaat tgtaaatgat attacagttt catcctgtgg gctgggtatt
    11041 gatacaggaa tagacacaat tatttctaat agtaggatat ctaagtgtca aaatggaatg
    11101 aggattagaa ctggaactag tataaataat gtaaggattg aaaaaataca gaaagttggt
    11161 cttataagta gtcaagttaa ggggtatgag gggtatggaa gttataaaat taataatatt
    11221 acgattgacc aatgtgggta ttgtggcttt tcatttgatt caatgaaaga tagttatttt
    11281 tctggtatta taagaaggtg tggtcagtat tattataata cagattatga cacatattta
    11341 agtattaaag atagagtaga agaagcatat tctttatttt atggaaatta ttttacagaa
    11401 tgtaatattg atttagttag tgataataag gataactggg atgatggctt agaatataaa
    11461 cataaagttt atgtatttaa aacccttcaa ataccaaaat taacattgag atgcaatata
    11521 gatgcctatg attatatcgt taaatcggta catggaggag gtaatttaat gttaaaaaat
    11581 aactttaaca attatatatt tattgattca tctagtccag aagttattaa tggaattaat
    11641 tttaattatc aaaagaatgg aaacctgtta aggttaaata atggtaaatt aagttataaa
    11701 aatggaaagg aagaaacagc tatattcaga cctaattatg gtcttattgt agcatcaaca
    11761 gattcagaat gtgaacaaat agaggctacc tatggagaaa aatgggagaa gcttggttct
    11821 gaagtaattg gaaaagaaac ggtatattat tataaacatc atgaaccaga aaaaacaaac
    11881 caaatacaat aaaatttgta ttatataatg atataaagtt ttaatatata gtgtttacct
    11941 ttaatgtaat attgatatat ttgtatagta ttaaatttgt taaaaagtaa aattgtaggg
    12001 tgcttgaaaa tagcaccttt tttagtttca ttataaaatt agttttgaaa gtttaggaat
    12061 aatgtatatt tatttttaaa gaattataac tttattaagg atattttaaa tgctagaaat
    12121 gaatcagtaa atcatactgg aattatctta aatgttttga ttaaattaag tatttagagc
    12181 tataattata ttaaaaatat attagaacat aattatatta ctatggaggt taaatatgat
    12241 agtaagtgtg agtagaagaa ctgatatacc tgctttctat agtaagtggt tttttaacag
    12301 acttaaagaa ggttttgttt atgtaattaa tccaatgaat ccaaaacagg ttagtaagat
    12361 agagctaaat ccacatactg ttgattgttt tgtattttgg acaaaggatg taactccaat
    12421 gttatatgat ttaaataaat taaaagacta taaatattat tttcactata ctataacatc
    12481 atatggaaaa gaagtagaga catgtatttt agataaaaga aaggtaatag attcatttaa
    12541 ggaattgtct agaaaaatag gcaaagaaag agtaatttta agatacgacc cgattttctt
    12601 aagtgaaaag tacacagtag attatcatat caaagcattt gcgagtttat gtaatcagct
    12661 tgatggattt acagaaaaat gtataataag ttttatcgat ttgtataaga aaacaaaatt
    12721 taatactaaa gcattacata taaagccaat agaaattaca gaaatagaaa cattatcaaa
    12781 agagatttct aagattgctc ataagcatgg gataatactt gaaatgtgtt ctgaagaata
    12841 taatttaagt aaatttggga ttggaaaaag taagtgtatt gatgatagat taatatcaaa
    12901 aattatagga tgtgaagtag aggtaaaaaa agatagtaca caaagagata tttgtggatg
    12961 tgttaaaagt gtcgatatag gacaatacaa tacttgcaga cattattgtc tgtattgtta
    13021 tgctaacttt aattatgcaa aagtagaaga aaactgtagg ttttataagg aagataataa
    13081 attactcata gatactttaa gagatgatgc aaaaataagc attagagata tgaagactat
    13141 taaagtagat aagaataaac aaattagtat atttgattaa aataaatata ctaattacct
    13201 taatatatta agaatataga tacttttgat aacaaaatta aacttaatag atattttcta
    13261 tataagaaag atggtcataa tatgtcaatt tgaccatctt tttgatataa ttaaaacaca
    13321 aataatttag taacaaagga ggcagaactt gaatttatta aaaaaatatc ctatacctat
    13381 tgcaagtttg attttatcaa tttttacttt aggtaactta ttacaatctt atagtgaatc
    13441 attacgtaat gtaattggat ttataggatt tattttgtat gcaatttata tagtcaaaat
    13501 aatattactt agaaagggtg ttaaagagca attagagaac cctttaatat caagtacatt
    13561 tccaactata acaatggcaa caatgatatt tgctacatat ataaaaccat tatctttaga
    13621 attaagtatt atgatttgga gcataggaat tgtgggtcat cttgtgttaa tagtcttatt
    13681 tagtaagaag ttcttagtta aattctcaat aaaaacggta tttccatctt ggtatatagt
    13741 atatgttggg atagttgcag ctagtgttac ggcaccagca tttaatcaac ttttgattgg
    13801 caaaattgct ttttggatag gatttgtatc ttatatagtt cttataccag taattttaaa
    13861 gagagtatgg ataatcaagg aaatgcctga accatcttta ccaagtatag ctatattagc
    13921 tgcaccaggg tcactattat tgtctggata tattaattct ttttcagata aaaataatgt
    13981 agtattatac ttgttattta tattatcaat tgtattttat attatagttt tgggatattt
    14041 accaaaatta ttaagattgc ctttttatcc gagttttgct gcatttacat tccctatggc
    14101 aataagtgca atgggtatga aactaatgaa tggttattta actaaatcag gtcattttat
    14161 aagttggatt tcatatgctt ctaagttaga agagataatt gctacagtga ttattttata
    14221 tgttttagtt ttgtttatta tatatcttag taaatctgaa actacatcta aagctaaata
    14281 agaattatta aatatgtgta tttaatagga atattgatat aaatatatga agataagata
    14341 aaccatagaa acttaattta gcttaatttt attggaggaa aacaaatgaa aacaaaagaa
    14401 gaatatttaa aagaagttag aaacatatca gaagaatatt ttagaaaagg agagtttttt
    14461 tgctctgaag cagttctcca gacaataaat gatgctttag gccaaccttt aagtccagag
    14521 ataactaagt tagcatcagg atttccgata ggacttggaa aggcacaatg tctttgtgga
    14581 gcaatatctg ggggagagat ggctttaggt atagtatatg gaagagttca tggtgaagct
    14641 atgaatccaa agatgtttga gcatgctaaa ggtttgcatg attatattaa aaaagagtat
    14701 ggtgcaacat gttgtagagc tattacaaag aaatgggatg gtgataactt tatgtctccc
    14761 gaaagaaaag agcactgtat taaaataaca ggtcaagtta cagagtgggt tgcagataag
    14821 cttatagaag atgaaaaact taatatcaaa tagtgtaata ttagttggtg aatttataaa
    14881 gttaaattta cttatagtat aagatacttg atattctcgt attgatagaa aaatttatag
    14941 catatgtatt taatgaaata tacttatatt tagagtgtgg tttaatttca taaatacata
    15001 tgctattatt ttaagtatat aagcaaattt atttaaataa atagaaacag ttaatttata
    15061 tgtacaaaac tatatatgat tagtgtaaat aaatacctat tttataatac tcaacataag
    15121 gacaccagac atgattagta ttataccaat aaaaatagca agccattctt ttttgttctt
    15181 cttttcttta aaaaatataa tccctcctaa agtggctact atcacacaac tctgagagat
    15241 agaaaaactt attgtaactc ccaatgtatt agtcgttatg aacataccta aatttgctat
    15301 tgaccacaat ataccagtaa ttaaattgaa taaaacattt tccctatata caatctcttt
    15361 agaaaaatac ttgacaataa tatatgaagt tatcaacata ccaacagatt gaggtaatat
    15421 aattctatat ccttcaatta aaaatatttg attagttaca acatataatg ataaaaatag
    15481 agatgataat aatacaactc caacacattt taacatattt ttatctgtag attttttagt
    15541 ttgataattt gtgagaagta taccaagtat tatacataat attgataaag aaccaatcag
    15601 taacatttta aaactggtcc attcattaaa caagattaca gctaataatg tcgtaaaagt
    15661 taattgtgaa ccatttgaaa ttggcattac tttagataca ctagcaattt gaattgcttt
    15721 aaactgcatt aattgtccca aactccagaa tatgccagaa atcatactta tacaaaatga
    15781 aaaaaatgta atttcaggat gtataaccag agttattata gtggaaaata taaacgcact
    15841 tatggatgta cctaaaagct gttcatgagg agaacttttc tttagatttg caactatggg
    15901 catcaatccc catcccatta caggaagtaa agcaataatt attgtcagca ttaaactatt
    15961 tcctttcata ttaaatttag acagctttta atttatcatc taaaattaaa gtgttcaata
    16021 tataaaaatt accgtattta atcaggaaat attagtagtt ttttagaaaa acattttcca
    16081 ttaaaataaa gacaaaggga aggagtaaaa ttgaaaatgt taactgatat gattcaaaat
    16141 cgtgaaatta aaataataaa gtttttatta aaagaaggtc atacaactgt aaaagcaata
    16201 gcagatgatt tagatgtttc agaaaaaaca gtttcaaaat ctcttaaaga agtagaagca
    16261 gtgcttaaaa agataggtct ttctttaata agaaaaccta agataggtgt ttatattgat
    16321 ggagatttaa aaaaaatact tccttaccta gataataaag gaagtcaaat tcctacaacc
    16381 agagaggaga gagttatcta tatatattca gtacttttaa agagtaatga ttatattact
    16441 atacaacaac tttctgatga actatatata agtcgtggaa ctattgaaag ggatttagca
    16501 gaagttgaaa aactgctaaa aaacgaaggg atttccatat ataagaaacc tagtaaggga
    16561 ataaagttaa acataagtga aagggataaa agacaactta ctgctaagtt tatacataaa
    16621 ttttggagta gaaactggta tattaagcaa gagcaagaaa gcttttatca agcatttgaa
    16681 aagatacagg cagatgtaaa tggtattttt tcggaagatg atttatcaat aattattgat
    16741 attcttagga atttcagcag agaaagaaaa tttgagttct ctgattatga atttcagtca
    16801 ttagttattc atttggcaat agctgtagag agaattagaa aaggcgaata tatagaagaa
    16861 agtttgtata ttggtaatat agaaaataat caaatgtcaa ataccaatta tttagcaaaa
    16921 caattagaag aaaaattttc aatattatta ccaaaatctg aaattggata tataaattta
    16981 catttagtag cggctgacca acatagagat actaatgaac taaaaaatac ttcagctaat
    17041 ccttgtgaga caagtacaaa taatatatca aatcttcggg aaattataaa aaatacattg
    17101 tatgagacag cttatgacaa ggatttaatt gatggattga cttcacatgt acattcatca
    17161 ataaatagac tgaaatatgg acttagtatt aaaaatccat atgtaaatac aatcaaacaa
    17221 aacttttctt tatcatttga gcaagcttta gatttaaaga aagtaattga atttatatat
    17281 gatattagga taaatgatga tgaatgtgct tatatagcat tacattttga agcttttaga
    17341 gaaagaatga aagaacttcc acaaaatatt agagtaattc tagtatgcag tacaggatta
    17401 ggttcatcta ggttattgtc tgcaagaata aaaaaatact ttccaatgat aaagattgaa
    17461 aaagtacttt ccgtacaaga attagtatcc agtaaaattg atgcagattt agtaataagt
    17521 actatttatt tagagttaga aacaattcca accatagtag ttagtcctat tttaaatgaa
    17581 agtgatataa gaaccttgga aagaaatata caaaatgttt caagttgtac ttctaagaag
    17641 tataaatctt tcagaaattt aatctttgaa gaaaatatat tgttgaatat tgaagcttca
    17701 aattatgaag atgcaattaa tcatataaca gataacctaa taaaaaaagg ttttgcaaaa
    17761 gaaggtatag ctaaaagtgc aattgagaga gaaaatttat cttacacgtc tttccagtca
    17821 atagctacac ctcatgctaa tcctagttat attaaaaaat ctaatatatc tgttgcaacg
    17881 ttaaaaaatc ctattacatg gggaaatgaa aaggtggaag ttattttctt tatttcatta
    17941 gaaaatgaca aaaacctaga gcttgatgaa atatatgaag ttttttatga cttcattgac
    18001 aataaaaaaa atataaatga catattaagt gcaacttcca caaaggaagt gtacgatttg
    18061 ctagtggagg agagtatata gtgaaaatat cagatgtatt gaaaaaagag caaattgttt
    18121 ttaatcttga tacaaataca aaggaagaaa caataaaaaa attaagtagt atatttgttg
    18181 aaaatgggat tgtggacaat gaagaagaat atgttaagtc tgttttggaa agagaagaac
    18241 attcaactac aggtattggt aatggtattg ctattcctca tgggaaaagt aatgctgtca
    18301 aaaaatcttc tataatattt gcaaagacta agcataagat tgaatgggat gctttagatg
    18361 aaaaaccagt agatagtatt ttcttattgg ctataagcaa tattgataag gatagcaatc
    18421 atcttgtact attatctaaa ttagcaacta aacttatgga tgatgacaat gtagatgcac
    18481 taaaattagc caattcagaa caagaaataa tcaatatatt tagtgaagaa ggagagatgt
    18541 agaaatgaaa agaaaaatta tagcagttac agcatgtgca acaggagttg cacatacata
    18601 tatggcagca caagcattaa agaaagcttc aaaaaagatg ggtcacctta taaaagttga
    18661 aacacaagga gctacaggaa tcgataatga gttaactaca aaggatgcac aaattgcaga
    18721 agtagtaata tttgcagtag atactaaagt cagaaatgct gaacgttttg agggaaaaga
    18781 aatactagaa gtaccagtta atgcacctat aaaggatgca gagaaagtaa taaatgatgc
    18841 attaaagtta attgacacta aataaatgga gggaaatgta atgttgaaaa agatagtctc
    18901 tgagttaaaa aagcatgtct taactggaat ctcatatatg attccattag taattgcagg
    18961 tgctatgata atggcgatat ctcgtgtagg tggttctttc tataatatac cagatatatg
    19021 ggatgctaaa tatgcagaaa gtgctagtag tattgttaga ttattacatg atttagatgg
    19081 gtttggaggt actgcgttag gattaatgtt tcctgtaatt gctgcattta taggattttc
    19141 tatagctgat aaattagcta ttgtaccagg tcttgttgga ggtatgattg ctaaagatat
    19201 aggtgcagga tttttaggag ctttggcagt tggtcttata gcaggatata catgtttgtt
    19261 tattaaaaat catgttaaat taccaaaatc agcagcttca atagtaccaa tatttatagt
    19321 accagtattt gggacattaa tcactgttgt attaatcaat tatgtgatag gtataccttt
    19381 tgccacatta aatactggat tagaaaattg gttaaatgga ttatctggta caaatcagat
    19441 actaatggca gctattatag gggctatgat ggcagttgac ttaggtggac cagttaataa
    19501 agcagcttta acaacatcac ttgcattatt aacaagtgga atttatgcgc ctaacactgc
    19561 tgcaatggta ggaatagtta taccaccact aggattggga cttgctacta tactagcaaa
    19621 acaaaaatat aataaacagt tacgtgaagc tgggaaatca tctcttataa tgggtttaat
    19681 tggagtaagt gaaggagcaa ttccttttgc agtagaatct ccattaaaag taataccttc
    19741 atgtgtaatt ggaacagcaa ttgcttcagc tatggcagtt ggtttaggtt cagtaaatgc
    19801 aactccaatt agtggatttt atggatggtt tacagtagag aattggccaa tgtatgtgtt
    19861 atcaatagca gttggaacag taattgtagc tggattagtt atagtattgc gtggaaatcc
    19921 agtgattgag gatgatgaat ttgaagatga tgattttgat gaagcagagt gggaatctta
    19981 aaatttaata actatttaag acgtgttata agctatattc aatatgaatc ttatactaaa
    20041 taaagtgtta aatattcagt ttagacttaa atataaaaga taaagaacag aggagaataa
    20101 atgataaaag cacatattgt taatcataca cactgggata gagaatggta ttttacttct
    20161 ggagatgcat tagtacttag tgaacaacta tttacagatg taattgatga attagaacgt
    20221 aatccagatg taagttttgt acttgatgga caactttcta ttttggatga ttatgtacaa
    20281 ttacacaaag aaaaaataga aatcattaaa aagttaataa aagaagatag actacatatt
    20341 ggaccatggt ttactcaaac tgatgcattc tttgctagag gagagtctat tttaagaaac
    20401 ttaatgattg gaatttttga aagtaaaaag tatggtaagt atatgaaaat tgggtatttg
    20461 ccagatacat ttggatttaa tgcacagatg cctatactat taaagcatgt aggtcttgat
    20521 aatatattct tttggagagg tatacatctt ggtaaacagg ttcaatcacc atattttaag
    20581 tggaaaagct taaatggaga aagttatata tatgcagtaa atatgccaca tggatatgga
    20641 actggtatgc ttttagagcc ttcattaaag tatgttaatg gacgcttaga cccagctatt
    20701 gattttatta aatcatatac tgatgtagat gaggtgttaa taccttctgg gaatgaccag
    20761 ttaaatataa taggtgattt caaaaataaa attaaagcga ttaatgatat cggaaagtat
    20821 tcatatataa cgagttcata tcaagagttt ttaaaatata taaagtccat agaatcacta
    20881 gaaagctata gaggtgaatt tagagaacct gtattagcaa gaatacataa atcaattggt
    20941 tctagtagaa tggatataaa attagcatgt gataaactag aaaacaagct gattaaaaga
    21001 atagagccat tattagtaat tgctaaaaag agcaaaattg aaataagcaa tcagttactt
    21061 ataaatacat ggaaaaaact tttggaagga caagcacacg atagtttagc tggatgtgta
    21121 actgatactg taacagatga tattcttcac cgtataagag aagcaaatga aatatgtgac
    21181 agtattgaaa atactattgt aaaaaagatt tcagaagggc ttaaattatc taaaaatgat
    21241 attttagtat ttaatacaga agctaagcgt tttaatggat ataaagagat acaagttgta
    21301 agtgatagta aaaatattta ttttaaagat aatttagatg ctactataat tgaagaaact
    21361 tatgtaaaac cgagagaaaa tgtattagaa gaaacacctg ctggtaatat atttattgaa
    21421 gaaccaggat attatatatt aaaagtaaga ataaatgtac agcttcctgc acttggttat
    21481 aaagttgtta gttttgaatt aagtgataaa gaaatggtgt cacttgataa atctaatgac
    21541 acttttatat ctaatgataa ttataagata atatatgaag atggaagctt aaatttacaa
    21601 cttaaagatg gaacttacat atcaaacttt ttaacattaa aagatagtgg taatgcaggt
    21661 gatacctatg acttttctcc actaaaaaat gatgagaata taaaactttc gtttgataaa
    21721 gtgtatacag aaaaagcttt aggatatgaa aaaatggtgg ttaaaggtac tactttacta
    21781 ccacttacat tagaagatag aaaaaacaaa tatcttaatg gtaaattaaa tgtaaaagta
    21841 agtattgtct tatctaaaga aaacccattg atggatgtaa aactttgtgt tgataacacg
    21901 atatataatc atagactacg tgttcatata aaaactgata taaaagataa taaaaacata
    21961 gcatcattgc catttggata tataacaaga aaaaatggag ttttagacaa ctgggaagat
    22021 atttacagtg aaatgcctat agatttagaa cctttggaaa gtaatattac gcttacaaac
    22081 caaaatagaa gttgtactgt atttactaga ggaataaaag agtatcaaca tattgaagat
    22141 gaaattgcat tgaccttact tgctactaca gatgaactag gaaagcctga tttattatat
    22201 agaccaggac gtgcttctgg tgatacaact aaaaaaggtc atataagaat taaaacagat
    22261 aaagctcaaa tgcttaagga attagaattt tcatttgcaa tatatatgga ttctaaatca
    22321 tttgatgaat tggaaattgc gaatttaacc cacaattatc taagagaatg tgtaaattat
    22381 caattacagg attacaattt ctttttatat cgtatagata ataagattca aaaatcaatt
    22441 gtcaataaaa ctgtaagtag agaattagaa ataatatctt taccagaaaa ctatcttata
    22501 tcagcatgtt atccttctta ttacagtaaa gataaattta taataagagt ggaaaatcca
    22561 agtagtaata gaatgatatt ggatgagcaa atatttaaaa atagaaatgg gaaaattgta
    22621 aatgcaatag aagatgtaga agaggaacag gtatatgaaa tagcaccttt tgacgttatt
    22681 tcaatattat tggatttata agtgtaaaag taagtttatt gtttaaagtc tgacagatat
    22741 taatctgcca gactttttaa tttgtataaa tttgggtaaa tattaatgaa tggttttagt
    22801 atttaagtat gaatttagta tttaatatag aatttattga agtagaataa ttagtttaac
    22861 aggataagca gtttaaataa agaattatta tattagagtg attttatata taatttaaaa
    22921 taataaaatt ttaataaaat gagttaaaga ttgtatatta atagagttga tgtagttaca
    22981 aaaaagttac caattgttat gtatttacaa aactgatata ttaattacat aaaatctaaa
    23041 atagtttgta ataggagtat atataataaa aattttaatt aaataaagct aggtatattt
    23101 ataatattaa taaatatatt gctataaaag tttgtttaat aaattaaata aagaaaagag
    23161 gagaataaaa tgtacaacat agatgatatg atatataatt gtccagtaga agctttatca
    23221 aatattttag gtaaaaaatg ggtggctata attatatgga aaatacaaga agataaaaaa
    23281 cgatttggag agctacaaag agctatacca gactgtagta agaaaatgtt agttcaacaa
    23341 ctagattttt taattgaaca ggggattata gtcaaccaaa aaaagttggt taataatgtt
    23401 gtagagtcaa catatttttt aagtgaatca ggattacgat tgcttcctgt catggaaaaa
    23461 atgattatgt ggagcaataa aaatttaaat tgtgataaat actaaaattt actctgtaag
    23521 taatgaaaat ttaattaagc tatgagtaga aaaatgtatt aaaattacat aattttttag
    23581 ttagatttta atttagaaat gggattgtaa tattggaaat aaaataagga ggaattttaa
    23641 tttgcaaaat gtaaatttat ttttagtatt tatagaaggt atagtatcat tcttttcacc
    23701 atgtatactg ccaattttgc ctatttattt gagtatattg tcaaatagta gtgtagaaaa
    23761 tttaaaagaa ggaaagacga gttttatagg aagttcttta tttaaaaata ctatattttt
    23821 tgctcttgga atctcaacaa cattctttat attgggttca tcagtaaaag tattgagtat
    23881 gttttttaat gaaaataaag atttaataat gtttattggt gggattataa ttattatcat
    23941 gggattattt tacatgggaa taattaaatc ttcaatattg aatagagaaa aaagatttaa
    24001 tgttaagttt aaagaaatga aagcaataac agcttttata ttaggtttta cgtttagttt
    24061 tggatggaca ccttgtattg gtccaatatt ggcatctgta ttagtcatgg tttcaagttc
    24121 aagtaatcat cttagtgcca atcttttaat agcagtatat actattggtt ttattttgcc
    24181 attcataatt acagctatgt tttacagtaa actatttaaa actatagata agataaaatc
    24241 taacatggaa attattaaaa aaataggtgg gattatactt atagtatcag gaatattaat
    24301 gatggtaaat ggatttggaa gtataagcaa acactttaat acgtcacaaa atagtaaaat
    24361 tgaaagcaag caagaagaaa ataagcgaga aaattctact gataaagaag aaaattctga
    24421 tggtaatgat agtcaaaaag actctaataa tgacaataat gataaagggt ctaatgatga
    24481 agatagaata aagtctattg attttacttt aacagaccag tatggtaaaa cacataaatt
    24541 aagtgattat gaaggcaaag ttgtattctt aaatttctgg gctacatggt gtcctccatg
    24601 taaagaagaa atgccttata tagaacaact gtataaagac tataataaaa ataatgatga
    24661 tgtagtaatt ttaggagtag cttcacctaa tttaggtaga gaaggttcaa gggaacatgt
    24721 agttaacttc ttaaaagacc aaggttatac attccctgtt gtacttgatg aagatggagc
    24781 attagcatat caatatggaa ttaatgcttt tccaactaca tttataattg acaaggaagg
    24841 atatgtaact cagtatattc caggagctat ggataaggca actatggcat catttattga
    24901 aaatcagaga aataaataaa agtatttaag tataagttta taaatcaatt taaggttaca
    24961 aaaaagtgcc taattgtacc acctaaataa tcttgttata gtaaaagtat aaacaataca
    25021 agaaagggtt gaattttata tgaaaaaaac atttgatgct ataataatag gatttggaaa
    25081 aggtggaaaa acattagcag gggatttggc aaacagggga ttaaaagtag ctttaataga
    25141 aaagtcaaac aagatgtatg gtggaacatg tgtaaatgtg gcatgtatac caacaaaaag
    25201 cttagaaaat tcagcaaaca gcgtaaaaac aaagaatata aattcttggg atgaagtaca
    25261 agctgaatat gagaaagcta tagataaaaa agaaacttta attacaaaac tcagagaagc
    25321 taactataat aaattaaatt caaatgagaa tgtaactata ttcacaggaa tgggaacttt
    25381 cattgatgaa aaaacagttc aagtcaaaac tgaaaatgaa atttatgagt tagtagcaga
    25441 taatatattt attaatactg gttcaagacc atttatacct aatattaagg gaattgaaaa
    25501 taagaatata gtatatgata gtgagtctct tatgaatcta agagttttac ctaaaaaaat
    25561 gacaattata ggagctggat ttataggttt agaatttgct ggtatttata gttcatttgg
    25621 agcagaagtt acaatattaa actctaataa tggcatttta ccaaatgaag atgttgagga
    25681 ttcagaagaa ataataaaat tacttgcaaa gagaaatgta aaaattgtta ataatgcaaa
    25741 tataaaagag attaaagaag tttctgaatt agcaatagta gaatatgaag tagatggtaa
    25801 atctaaagaa ttaacaagta atatgatact tgtagctaca ggaagaaaag caaacactga
    25861 agggctaggg ctagaaaatg caggtataga attaaatgaa agaggattta ttaaagtctc
    25921 agaaactctt aagactaata aagagcatat atgggctata ggagatataa atggaggtcc
    25981 acaatttact tatatttcat tggatgatta tcgcatagtt attaatcagt tatttggaga
    26041 taagactaga acaactaatg atagaaagaa tatcccaaat tctatattta taagtccagc
    26101 attttcaaga gttggtctta atgttaagca agctaaagaa aagggatatg aagtattagt
    26161 ggcaaagatg ccagtagaag caattccaag agctaaacag attggcaagg cagatgggtt
    26221 tataaaaata gtgatagata aaaaatccaa taagatactt ggagctagta tgatatgtga
    26281 aaattcaagt gagattatcc atcttattca attagctgta gatttagaag tagagtatac
    26341 atatttaaga gaccgtgttt atgcacatcc aactatgaca gaagctctta atgatattct
    26401 atctcctaat atgataaaag aagtataaca taagtttaat atataaacct aaaattatca
    26461 ttataagtag attttagaaa gagactatct caaaaaatag agataagtct cttttttact
    26521 gtttaaataa taaaaatata tttgatttta taaaagaaga ttattttatg agctaaaaag
    26581 tttatttaga aacactacct atttatgtta taaagttata aatataatat agaatctatt
    26641 attctgtatt attaagtggg gttaagcgtt ataattaatt ataaatattg aataatctaa
    26701 atggataaga taatggtatt aaaaattatc ttaaaaggtg aaataatctg tatttaatgt
    26761 agtacagtta tatctttaaa gcaaggtggt gaaatgatat aaataagata tctaaaagag
    26821 ggtttgatga tagtgataaa agatggtttt caagtaaaga attaacaaga cttataaagg
    26881 ctaaagaaga aatagaatgg ttattaaata gggattacaa aatagaccca gtcgttactt
    26941 ttgttggtga tagatatcag ttttctataa gacaaagaga tgcattaaaa agagcagtct
    27001 gtactgatga aaaaaatatt ataagacaat caaagaggct ttctctagat aaaatcaaag
    27061 aagataccgt aaatatagat ggctttaatt tgataatatc catagaggta ggattatctg
    27121 gtggtactct agtaatagga agtgatggca atataagaga tttagcaggt cttaggggaa
    27181 cctataagat tatagataaa acagaagaag ctttgaatct gataggtaaa ttttttaata
    27241 aatacaatgc acagaatctt aaattttatt tagatgctcc agtttctaat tcaggaaatt
    27301 taaagtatag gatattagag catgccaaaa cttggggaat tgaaactgaa gtagaattag
    27361 taaaaaatgc agatgttgta ttggaaaaat tagatagagt agttagtagt gatgcagtca
    27421 tagttgacaa gtgtataagt tattttaatg tggctagagg tataattgaa gagtatatta
    27481 aagaatgtaa tatcataaat ttaaataaat agataaatat aggtgagaat atataagtat
    27541 atgtagcaat attcttagta atacaagtaa agtttgaaaa taggagaacg aatacatatc
    27601 gaatttcata aaatgttgta aaaagacata tgagaggatg gatatgtgtg aaattaacta
    27661 aactaaatat taaaaaatat agagcaccta tatataaata tgctttagca aatataaatt
    27721 tgaggtctgc taaatctacc aattcaagta tcataacagt aatacctcag ggagccaaaa
    27781 tggaagtatt ggatgaggaa gatgactgga ttaaggtgat gtacaattct caagaaggat
    27841 atgtgtataa ggagttggta tcagtaagcg aatatgcttg gagtaactta aacttaagag
    27901 aagataaatc tacaacttcc aatataatta cagtaattcc tgaaaaatct agggttgaag
    27961 tccttcaagt agatggagat tggagtaagg ttgtatatga tgataaaata ggatatgttt
    28021 ttaattattt tttatcaatt gatgggaata aaccaaatga attagattat aagtattttt
    28081 atactgacat gattaaattt gttaacgaaa ataatattaa aagtactagc gattatctaa
    28141 ttgtaacaga ccttagaaat aaatatactt atatttttaa gaaagataat ggaggatggg
    28201 gacaacttta caagtggcaa tgtactatag gcaagcctga gactccaact ataacaggta
    28261 tattttatat aagtggaaga aaaccttctt ttggaacgga tgagtattca gttaagtatg
    28321 ctactagaat taaaggtgga tattactatc attctgtact atatgattca acaggaagtt
    28381 atataataga tggcagactt ggtgaagctc ttagtcatgg atgtataaga ttaagtacgg
    28441 aaaatgcaaa gtggatatat gataatatac cagatacaac aacagttata attcattaag
    28501 aaaaattttg agttttttat tgggataatg attcttaaat taataggatt atatattaac
    28561 ttctcataaa gagtactatt taaaaagtgt aataagagag tgctattaac aaaacaaaga
    28621 tattttgagg cactctctta taaatataca taattaagat attttcataa acaattctat
    28681 aatcttatct ttatcaaaga aaacaggatt agcaccagca caagcatctt tcatagagtt
    28741 ttctgcaagc aattcaaaat taggattatc aacacctaca tcttttaaag attttgggat
    28801 tccaacctct tcagaaagag cttttatctt atctattaca aagtcaacac actctttatc
    28861 tgatttatta tttacatcca ttccaataac ttcagctatt ggtcggaatt tggcaggggc
    28921 tgatttagca ttttcctcct caactattgg aagtagcatg gcattacata ctccatgagg
    28981 taaatcatat aaacctccta attgatgagc catagcatgt acatttccaa gacctgcatt
    29041 actaaaggca attccattta aaaaacaagc ataacacatt tgttctctag cttctatgtc
    29101 attaccattt ttaacagctc ttggaaggta tttaaatatc tgctttatag catataaggc
    29161 agttgaatct gtaacatcat aagcaccatt agctactaca gcttctatag catgagttag
    29221 tgcatccata ccagttgctg ctgttaaatt agatggtttg ctaatcatta attctgggtc
    29281 atttactgtc attgtggcta gtgaattggt atcaaccatt atcatcttta catgtctgtc
    29341 ttcatcagtt ataacatagt ttattgtaac ttcagcagat gtaccagcag tagtagttat
    29401 agctatgata ggaagacatt ttttagaagt tttgttgata ccttcatagt ctctaatatc
    29461 tcctccatta gtggctaata tagagatagc tttaccacag tcttgtggag aaccacctcc
    29521 tatagttata acgaagtcgc aattttctga ttttaatatt ttaagtcctt tattcacatt
    29581 agttacagtt ggattttgtt taacttcatc ataaacaaca aagtctatat ctatctgaga
    29641 taaaatatct gttacttttt ttacagtacc atttgaaact agaaatttat cactaacaat
    29701 aaatgctttt ttagttccta atgatttaat tgaatcttta gcttcattaa gacatccttt
    29761 cccaagtaaa tttattggtg gtacataata aatgctcata actaaaatct ccttttattt
    29821 tcattgattt ttatgtaaaa atatatatct aacaattttc actaatttac tcataatata
    29881 tcgtatctta gattgagata gggttgctgg ttcttatata agaacttaat tcgtaattac
    29941 gattatgatt agaatttaac atagtatttt tatcatgtca ataaaaatat aaaaaatatt
    30001 ataaaaaaaa caaagttact gtagttaaat cagcaacttt gtttttatag tataaatcaa
    30061 ataaatacat tagtttaaaa caaaattttt aattattatg ttttgatatc ttccatatac
    30121 agaatattaa tctaatttat tacaatgtct tcattacgtt cttccatcat tgattgaata
    30181 tttttctgag catttaagag taattttttt atcagttcta ttttttcgtc attagctcta
    30241 aataaaggca gtgttacact aacagcagct cttatacatc cattttttct tattggtacg
    30301 gcaagacatt ttaggtgttc cattgattcc tcacactctg aagctatttg tgttctttga
    30361 acttcaagaa gctggtcgta taaaacatca aaatctgtaa ttgatttttc tgtaaaacct
    30421 tttagaccat ctggatataa gcttattagt ttttccttgt caaaatcaca taaaagagct
    30481 tttccaagac ctgtacaatt agcaggtatt ttttttccaa cataagaaat aagtctaatc
    30541 ggttctggag aatctacttt tccaatataa agaacttcat tattgtcaag tataccaagc
    30601 tgacaagttt cagaacagga attaactata ttagtcattt cgtcaattat atagttaaaa
    30661 atagttcttt tttcaaaaaa tgaagaccct aatgtgtaag aacgtatccc tatagaatat
    30721 ttggatgtgt ttttatcaat agtaatgtat tttctagcat taagtgtatg aactatagga
    30781 aagatgctac tttttggagc attgataaca tctgctattt ctgtaagagt atatccattt
    30841 tcggattctg ctaatgcttc taaaatatct aatatcctag ctgttgggcg atgattatct
    30901 gaagtcataa atttccccct ctttttatta atttaaagta ttagaatatg ttgattttag
    30961 cacactatac gatattttac aatgtagaaa ataaatatgt aaatttatat ataaataagc
    31021 tcttgattta gatacagata gatatttttt tagactgtac attaaggaat aactaatttc
    31081 caaaatatac ctaaagaaag taagtgatat ttagttatat aatttaagta aatatatgca
    31141 atagttattt ttatatgtaa atatcaaaat attatgaact tatattttat ctatttttta
    31201 cactgtataa aaactttata gttttgaata ctatataatt ttaaatacgc tgtatttttg
    31261 catataagga agtcatttga taatatacaa ggtattatca gaaagtttga aaaaattttg
    31321 acaaaaattt atttttttta ttgactcata aataggaaaa tgctatcatt aattcaaaga
    31381 ttcgtaatta aaaacttagt tcttaaatac gaaccgaaac ggccgttaga attaaagcta
    31441 aaaacttaat aagagtaatt aaatatcatg ttttaggagg attaacttta tgataatgga
    31501 ttttattaaa aaaattcctg caggtatgat gatagtgcca atgtttatag cagctttcat
    31561 taataccttt tgcccagata ttgtacaaat aggttcgttt acgacagcag tattttcaag
    31621 tgttgggtct gcaactataa ttggggttca actggtatgc ttgggaactc agcttcaatt
    31681 tagagaaatg ccaaaagttt taaagagagg tggtatactt cttggttcta aatttgctat
    31741 aggtgctttg ataggtatac ttataggaaa gctatttgga atggatggtg tccttggtct
    31801 gacaacattg gctgttataa gtgcagttac aaatagtaat ggtagtatat atctttcatt
    31861 gatgacttca tatggagatg aaacagattg tgcaactatg agtttactta ctttaaatga
    31921 tggaccattt tttacattaa tagctcttgg gacttcaggt cttgcaaatg taccattgtt
    31981 gtcattattg gctgcaatag tgccaatact tgttggtatg ataataggaa attttgacaa
    32041 aaaaatgaaa gagtttttag agccagggtg taatttactc gtaccttttg tagggtttgc
    32101 acttggtagt agcataaatc ttgcaagtat actaaaagga ggtttatcag gagtattact
    32161 tggattaata tctgtatttg taggaggtat atttatagta ttctgtgata aatttatagg
    32221 aaaacgtcca ggatatgcag gatgggctgt agctacaact gctggaaatg caattgctgt
    32281 acctgctgct gttgcattag ttgacccatc atggcaacca tatgttgcaa cagctacaac
    32341 acaagtggca gccgcagtag ttgtcactgc aatattagta cctcttataa ctagttggtg
    32401 ggctaagaag tatggatgtc ctaagttccc aaaacaaaat gaggatggta caataacagc
    32461 ttaatttata ttacagtgag ttgttggtaa aataataaat tctttaatta gattttagga
    32521 tattaataaa attatattat ttataaagga gagcaagaat aatgataaat gcagtgataa
    32581 ttgatgaaaa agataatgtt gcagtagcta ttgaagcaat agctaaaggt agtgaaatta
    32641 gttttaaatt aaaagacaaa acagtaaaaa cgattacagc actagatgat ataactattt
    32701 atcataaatt ggctataaaa gatatggcca aaggtgataa agttactaaa tatggtgaac
    32761 atataggaga agcaaatcag gaaataaaaa gtggccaaca tgtacatatt cataatgtta
    32821 gaagtgttag agaggatttg gataaacaag ttatgactaa tatataaaat aatatttata
    32881 gagttataaa agttgtattc attgctttta gtgtcacata gtttttaaat aggtttttag
    32941 ctgtttgatt agtgagtgta ataaaaatta taatatataa aaattataga aagtatatag
    33001 actattttag gaggaaatca gtatgaaatt ttatggatat aaaagaccag atggaagagt
    33061 tggtataaga aaccacatat taatattgcc tacgagtgtg tgtgcatcag atacaactag
    33121 aataatagca tcacaagttc aaggggcagt tacctttaat aatcagaatg gatgttcaca
    33181 agtacattca gaccaacaat taactatgga tgttatggct ggttatgcag caaatccaaa
    33241 tgtatatggt gtaatagttg tatctttggg atgcgaaaat tgccaaaatg atttggtagt
    33301 tgatgcaata aaagagagaa caaataagcc aataaaaact cttgtaatac aagaagaaca
    33361 tggaacttta aaaactatag aaaaagctgt tcgttatgct agagaaatgg cacaagaagc
    33421 ttctttgctt agaaaagaag aatttccaat atctgaactt atactaggaa ctgaatgtgg
    33481 tgggtctgac ccaacaagtg gattagcagc aaatccatta ataggtgaat taagtgataa
    33541 acttgtcgat ttaggagcaa catcaatttt aagtgaaact actgaattta taggtgcaga
    33601 gcatatacta gctaggagag cagtaaatga agaagtaaaa gaaaaaatat tacatatagt
    33661 acatagatat gaaaattcat taaaattggt tggagaagaa gttagagaag gtaatccatc
    33721 tcctggaaat atagctggtg gtttaacaag tttagaagaa aaatcacttg gttgtataca
    33781 taaaggtggt catagacaaa taagtgaagt atatgattat gccaaacaaa ttgataagaa
    33841 aggtcttgtt ataatggata cccctggaaa tgatgcttca tctgttgctg gtatggtagc
    33901 aggaggagca caaatagtgg tatttagtac aggaagagga acaccatcag gaaatccaat
    33961 agcaccagta ataaaaataa ctggaaataa aataactttt gcaaatatgg aagataatat
    34021 ggattttgat gctagtccag ttatctatgg tccacaaaca atggaagaat taacagatga
    34081 tttgttaaat atggttgtag atgtagcaaa tggaaaacaa tcaaaagcag agtcccttgg
    34141 atatactgaa atggcaatag ctagagtatg caactatgta taatctttaa gtaagaaaat
    34201 aaatacatag aattttaata aagggtgaga ttatgaagtt tgtaagtttt actgaaggag
    34261 aaaatgaatg taaaagaact ggagtttttt ctaaagatga attatttatt atagatgtta
    34321 actctttaaa tctcagtaga aattttaagg atttaaatga attaattcaa aatgtatcta
    34381 gtgaagatat tattaagttg aaaactatta taaatgaaag tacattctat aattatgatg
    34441 tttttaaaag agagagatgt acggtacatg ttcctataga aaaaccaata catgatatct
    34501 tatgtgtggg agtgaactat aaagaccatc ttgaagaaac tcaaactcac tttgatgata
    34561 gttttgaaga accacaaaaa acggtttatt ttacaaaacg tgtgacaaag gcaataggtc
    34621 cagaagatga gatagatggt cattttgaga taaataatca actagactat gaagttgaac
    34681 ttggagtagt aataggtaaa acaggtgtta atattagaaa agaagaggta gaagattata
    34741 tctttggtta cactgttata aatgatattt ctgctagaaa gttgcaaaaa gaacatgttc
    34801 aatggtttag aggaaaagga ttagatacat ttacctcttt aggtccatgt attttacata
    34861 aaagtgcagt tccttttcct ataaaactta aagtatgtag tagggtaaat ggtgaagaaa
    34921 gacaaagttc taatacagga ttattcatat cagacgtttc agacatagtt tcagaacttt
    34981 caaaaggtat gaccttagag gctggagaca ttatagctac tggaacacct tctggagtag
    35041 ggatgggatt ttcacctcct cgatatatga gtagtggaga tgtagttgaa tgtgagattg
    35101 agagtattgg aattttaaaa aattacatta agtaactgtt ttaatataat taatattaaa
    35161 gctaaatctg atgtattaat gaacatatta aaataaattt taggagttta aattatgaaa
    35221 gcagaatttt tgacaccagt tgttacaata tttgatgaag aaggaaattt agataaagaa
    35281 ggtaataaaa aagtgtatga ccatcttata gatggaggtg tagatggact agtcataatg
    35341 ggaagtactg gagagttctt taatatgtct atggatatgc aaaaagaact tatagattta
    35401 gtaactagtc atgtaaataa gagagtaaaa gtgtttatag gtacaagtcg tatgtgtata
    35461 aatgaatcta tagaactttc aaactatgca cataatagtg gagcagatgg tgttatgata
    35521 ataagccctt attatttctc tcttgatgat gatagtattg agttatttta tagtgaaata
    35581 gctaagaata cagaggctga aatatatttg tacaattatc cagatagaac tggttatgat
    35641 ttaagtccag aacttacatt aagacttgca agaaaacata aaaacatagt tggatataag
    35701 gatacagtaa ctttgatggg acataccaga gatttaataa atactatgaa aatagagttc
    35761 ccagaattta aggtttatag tggttatgat gaaaacttta tacataatct aatgtctggg
    35821 ggagcaggtt gtataggggg gttatcaaat ttaattccag aagtttgctc caaatgggct
    35881 gaagcttaca ataatagaga tatggagaag gttatagaaa ttcaaaaata tattaataaa
    35941 gcaatggatt tttacttaat aaccaatcca tttataccag caatgaaaaa agctatgaat
    36001 atcagagggt tacaaataag tgattatatg actgctccat ttgtaaaacc aaatgaaaaa
    36061 caagtatgtg aaattaaaaa attgatgaat gaattaaata tatgttaaat caaccttgca
    36121 aagaggtcaa caataaatta caattatctt aataaaaatt aaattaaaat aatactaggg
    36181 tactatatta gtttaatagg gaaagtgatg aaaattcact acagcccccg ctactgtata
    36241 cggatacgaa atcaaaatcc actgaaattt ataaaaattt tgggaagggt gatggaggaa
    36301 aagccgtgag tcaggagacc tgcccagtat tatataagtt caatttaggg tggagattgt
    36361 aatttataat aaaatgaagt atcaattcgt ttttgtaaag ttgatatatg aacttctcct
    36421 tattaaatta ggagaagttt tttattttat taaaaggtta tacttattaa agatgacttt
    36481 aggataactt taataagtat actttaaaaa gcatatatta ttaaagtttt ttagccactc
    36541 ataaaaaata agatattgga gtgaaagaga tgaaatttaa atcaaatgtt ttaaagcagt
    36601 tttttttaat tgctatggta atttttatgg taggaggtct tatagggtgt agtaaaagtg
    36661 acaaagagaa taaacaagct agtactggta agaaattaga attaaaatat gctagtggat
    36721 ttagtgttga agatttagaa gatggaatta aaaaagtaac tgatggagat aatcgagaac
    36781 tgattttaat cccaaagaag attaaaattc ctgaaaaata taaaaatgct aatatagtta
    36841 gaacgcctgt ggacaatgta ttaatagcat ctacaacaca ggcttgttct ctgagaacaa
    36901 tagatgaact agatagtata aaagccgtaa caacagaaga gcagtactgg actattaaag
    36961 aaattaaaga tttaatgaaa gatggaaaaa tacattacgt tggttcaaat ttagcaccag
    37021 attatgaaaa aatagtagac ttaaatcctg acttaactat agtgagtagt ggtttatcag
    37081 aggctgatac aaaatttatt gacaaattaa aagaattagg gcttaattat gttgtagaca
    37141 atgagtataa agagcaaaat ccatttggaa gaatggagtg gacaagttta atagctgcat
    37201 tttatgataa agaagatatg gctactagtg aactaaataa aactgtacaa aaggtagaag
    37261 aagtaagtaa aaaaataaaa aacaaagcta aacctaaagt tgtatgggca tctgtatatg
    37321 aaggaagtgc ttatatagct cctaaagatt catatgtgga taatatgata aaaatggctg
    37381 gtggaataaa tgcatgtgct tcaatagatt caaatgaagg gcgagtaagt attgaacaat
    37441 tgcatgaagt tgcaaaagat gcagatatat ttgtatactc atcaagttca gattatgcac
    37501 caaatttaga ttctataaaa tctagtgcac ctgtattggc tgatttggat gttgtaaaaa
    37561 acgggaacct atgggtgttt gctccagatt attatcaatc agtagataag acggacgaat
    37621 taatagtaga tttagttgag atgttccacc caggtactac aggtgaaaaa atcaaacatt
    37681 atataaatta tgataaatag tacagagagg acacaaaaag ataggtttta tagattttag
    37741 ttaatatagc tttgctaaga ggagtataca aatggatttt atcaataaaa tgagaataag
    37801 gaatacaaca atatttatag tattaattat aagtatgata cttacttttt tagtgtgtgt
    37861 agcatttggc tctgtaaaaa ttgaatttaa agaagtaata gatgttgtat taagaggtat
    37921 ggataataca gaacattata aaataatata caatataaga atccctagag tacttgcaac
    37981 catggtaggt ggagcatgcc tagcagtatc aggtatttta ttacagatat tttttaaaaa
    38041 cccaatagtt gaaccgtata tattaggagt atcttcaggt tctactttat cagtagctct
    38101 catgattttg ggaggataca cattgggatt tgagagtgta aattcaatga gtatatttgt
    38161 agcagctttt ataggagctt taattgtaat gtctatagtc attttatttg caaataaggt
    38221 aaagaatata acaactcttt taataatagg aattatgacc ggatatgtat gtagtggtat
    38281 aacaaatgtg ttacaatcat ttgcaaatag tgataaatta aaagaattta ctatgtggtc
    38341 tatgggaagt ttttctggat ttacatggga aaaggtagaa atacttataa tagttggtat
    38401 attaggtctt ttgatgactg tattcataat aaaaccttta aatgcttttt tactaggaga
    38461 agagtatgca aaaagtattg gcataaatat aagatttttt agagttttga tagtatttat
    38521 atctagtata ttagcagcaa tagtatcagc atttgcagga cctatagggt ttattggact
    38581 tgcagtgcca caaatttcaa aaatgatatt taaaacatca gataatagag ttttgatacc
    38641 agtagtaata tttttaggca gtatagttac agctctttgt gatttaatat caagagtaat
    38701 tttatcacca gtagaattac caataagtac gataacatct ttattaggag ctccaattgt
    38761 agtaatgtta ttggtgaaaa ggaaggatag ggcttagaca tgttgaaaac taataattta
    38821 agtgtaggat acaataacaa agttgttata agtaatataa atgtagaagt aaaaaatgga
    38881 gaaatactat gtcttttggg aagtaatgga gctggaaaaa ctaccctatt aaggtcctta
    38941 tctaaactta tatcaccaat aaaaggtgaa atatatttga atggtgtaaa cataaattgt
    39001 atatcaagaa aagcattatc aaaaaaaatg gctttagtac ttacaaatag attattaggt
    39061 gatttaatga ctgtacaaga tatagtcaat ataggaaggt atccatatac aggatttttt
    39121 gggagtctat ctaagaaaga tttaataatg gtagatgaag cattggagtc tgtagatgcc
    39181 ttacatttaa agaaaagata ttttgatgaa ctaagtgatg gagaaaaaca aaaggtgttg
    39241 gttgcaaggg ctcttgttca agaaccagaa ataattatat tagatgagcc tacaactcac
    39301 ttggatataa aacatagatt ggaactcata aacatactga aaaaattaag taaagaaaag
    39361 tcaatatcag taatactttc attgcatgaa atagacatag cattaaaaag ttgtgataaa
    39421 gttgctctta taaaaaataa taaagtcata gcttatggtc agcctgaaga tgtagtagat
    39481 gaggatatta taaattcgct ttatgaattg gatgataaaa actttaatag tcttttaggt
    39541 tcagtagaaa ttagtaataa atctaaaaat gaagttttta taattggtgg tgggggtaaa
    39601 gcaacaccaa tttatagagc ttttactaaa aaaggtatag gtctgtattc tggaataata
    39661 catgaaaatg atattgatta tgaaattggt agaactatgg ggataaagat gtttacagaa
    39721 aatccatttg agcctataag tgatgagagt ttcgatttgg ctattagaaa tttaaatgat
    39781 tccaagatta taatagatac aggatttagt gtaggagaga caaataagag gaatatagat
    39841 attataaaag aggctttaaa attggataaa aaagtttatt cttttagaaa tagagatgag
    39901 agtaagaagt attatgacag tttggatagt aagatagaac atatagataa agtttctcaa
    39961 ataatcaata gtgcagatat aaataattta ttataattta tgaaataaaa tgtacttatg
    40021 tgctatagaa aaaagagtat agattataat ttttttgaaa ttacaatcta tactcttttg
    40081 ttattttgtt tatgtattta tatatttata aaactttagc attttataga taaccaatag
    40141 cttcctctct agttaaaaaa aagtgaatcc ctgtagtcga ttctttccat ctattttcat
    40201 taaaatcttc agctacaatc gtttctccta ctctatatat aaaattttca tcagcatatg
    40261 cacgagcact tttaaaagat tctttaccat ctatactagt aattgacagt acttttgcct
    40321 tatcacatct acaagcgttt gatgtagcag acactctttt tgcgtcgctg ggtattaata
    40381 attgtaccat tctaaaatta aaacattttt tatagcctat aaatgctcct gtttcaggac
    40441 aatacaattt aaaaaaacgg gtactttcat ctgatattac tccatttaaa gtagcatgtt
    40501 ctaaatttga attttctaaa ttagctcctc taatatctga gttagataag tcacaaaatc
    40561 taaaacttgc accacttaag tctgcatttt ttaatataga attttttaat gaacaatccg
    40621 aaagataaga attattaaaa atagagtttt ctaaatctgc tttttctaaa tttaaattga
    40681 taaggtcaga ccacgaaaag tcaatattac ttaagcagta tcctcttaag tccatatttt
    40741 ctaatcttaa ttcttttaaa tctgcctttt ctccagcatt attgttttta agccataaat
    40801 aatgtttctc aagtgttttt tctaattctt gtttagaaat agttttcatt aaaatcacct
    40861 tcataaa
//
LOCUS       DAGSWI010000021        40866 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.021, whole genome shotgun sequence.
ACCESSION   DAGSWI010000021 DAGSWI010000000
VERSION     DAGSWI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.021"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            144..761
                     /locus_tag="MJF80_001001"
     CDS             144..761
                     /locus_tag="MJF80_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBZ0279674.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(918..1370)
                     /locus_tag="MJF80_001002"
     CDS             complement(918..1370)
                     /locus_tag="MJF80_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HBZ0279675.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1818..3176
                     /locus_tag="MJF80_001003"
     CDS             1818..3176
                     /locus_tag="MJF80_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBZ0279676.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            3449..3829
                     /locus_tag="MJF80_001004"
     CDS             3449..3829
                     /locus_tag="MJF80_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBZ0279677.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            3954..4154
                     /locus_tag="MJF80_001005"
     CDS             3954..4154
                     /locus_tag="MJF80_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279678.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            4350..5078
                     /locus_tag="MJF80_001006"
     CDS             4350..5078
                     /locus_tag="MJF80_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0279679.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            5100..6017
                     /locus_tag="MJF80_001007"
     CDS             5100..6017
                     /locus_tag="MJF80_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBZ0279680.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTKEEAEQQILNCFLK"
     gene            6159..7343
                     /locus_tag="MJF80_001008"
     CDS             6159..7343
                     /locus_tag="MJF80_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBZ0279681.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTYLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            7450..7638
                     /locus_tag="MJF80_001009"
     CDS             7450..7638
                     /locus_tag="MJF80_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBZ0279682.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            7787..8437
                     /locus_tag="MJF80_001010"
     CDS             7787..8437
                     /locus_tag="MJF80_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBZ0279683.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            8540..9478
                     /locus_tag="MJF80_001011"
     CDS             8540..9478
                     /locus_tag="MJF80_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBZ0279684.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            9504..10355
                     /locus_tag="MJF80_001012"
     CDS             9504..10355
                     /locus_tag="MJF80_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBZ0279685.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDSIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
     gene            complement(10640..11374)
                     /locus_tag="MJF80_001013"
     CDS             complement(10640..11374)
                     /locus_tag="MJF80_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBZ0279686.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            11499..12215
                     /locus_tag="MJF80_001014"
     CDS             11499..12215
                     /locus_tag="MJF80_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBZ0279687.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            12349..13041
                     /locus_tag="MJF80_001015"
     CDS             12349..13041
                     /locus_tag="MJF80_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0279688.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            13031..14212
                     /locus_tag="MJF80_001016"
     CDS             13031..14212
                     /locus_tag="MJF80_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBZ0279689.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            14494..14949
                     /locus_tag="MJF80_001017"
     CDS             14494..14949
                     /locus_tag="MJF80_001017"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBZ0279690.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            14939..15664
                     /locus_tag="MJF80_001018"
     CDS             14939..15664
                     /locus_tag="MJF80_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBZ0279691.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            15636..18017
                     /locus_tag="MJF80_001019"
     CDS             15636..18017
                     /locus_tag="MJF80_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBZ0279692.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(18222..19451)
                     /locus_tag="MJF80_001020"
     CDS             complement(18222..19451)
                     /locus_tag="MJF80_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBZ0279693.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            20051..20965
                     /locus_tag="MJF80_001021"
     CDS             20051..20965
                     /locus_tag="MJF80_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279694.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            20989..21624
                     /locus_tag="MJF80_001022"
     CDS             20989..21624
                     /locus_tag="MJF80_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBZ0279695.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAGGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            22000..22716
                     /gene="pyrF"
                     /locus_tag="MJF80_001023"
     CDS             22000..22716
                     /gene="pyrF"
                     /locus_tag="MJF80_001023"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBZ0279696.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVKEIENA"
     gene            22742..23785
                     /gene="carA"
                     /locus_tag="MJF80_001024"
     CDS             22742..23785
                     /gene="carA"
                     /locus_tag="MJF80_001024"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBZ0279697.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            23838..27044
                     /gene="carB"
                     /locus_tag="MJF80_001025"
     CDS             23838..27044
                     /gene="carB"
                     /locus_tag="MJF80_001025"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBZ0279698.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            27301..28350
                     /gene="carA"
                     /locus_tag="MJF80_001026"
     CDS             27301..28350
                     /gene="carA"
                     /locus_tag="MJF80_001026"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBZ0279699.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            28543..31749
                     /gene="carB"
                     /locus_tag="MJF80_001027"
     CDS             28543..31749
                     /gene="carB"
                     /locus_tag="MJF80_001027"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBZ0279700.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            32249..32935
                     /locus_tag="MJF80_001028"
     CDS             32249..32935
                     /locus_tag="MJF80_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0279701.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            32922..33911
                     /locus_tag="MJF80_001029"
     CDS             32922..33911
                     /locus_tag="MJF80_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0279702.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSNTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            33994..34680
                     /locus_tag="MJF80_001030"
     CDS             33994..34680
                     /locus_tag="MJF80_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279703.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            34680..37226
                     /locus_tag="MJF80_001031"
     CDS             34680..37226
                     /locus_tag="MJF80_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBZ0279704.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(37382..38239)
                     /locus_tag="MJF80_001032"
     CDS             complement(37382..38239)
                     /locus_tag="MJF80_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0279705.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(38260..38925)
                     /locus_tag="MJF80_001033"
     CDS             complement(38260..38925)
                     /locus_tag="MJF80_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBZ0279706.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTLKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            complement(38925..39797)
                     /locus_tag="MJF80_001034"
     CDS             complement(38925..39797)
                     /locus_tag="MJF80_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBZ0279707.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            39965..40708
                     /locus_tag="MJF80_001035"
     CDS             39965..40708
                     /locus_tag="MJF80_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBZ0279708.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
BASE COUNT        15860 a         4659 c         7314 g        13033 t
ORIGIN      
        1 ttttttaagg atataaagta aaaaaatata caatatcaag aaaaaaagat attgggtatt
       61 taaagtaata ctttatttaa ttttaagtaa atcgggtatc tacatatata gatactaatt
      121 tcaaagaata ggagggtgtt tatatggaaa tattcgcaat aggacattca aattatccat
      181 atgataagct tattaatatg ataaaaaaat atgacataaa ttgtgtagtt gacattagag
      241 aaactccata ttctaaatat aatattcaat acaataaaga ggcatttaac gaatctctta
      301 gaaattccgg atttttatat atatacatgg gaaaagaatt tggagctaaa agaactaata
      361 aagatgtata tacgcaagaa ggttatgcag attttgagaa agtagcaaaa gaagatattt
      421 ttttaaacgg aatagaaaga ttaaaaaagg gttgtcaaat gggttatagg atagttttat
      481 taggagctat gcaagaacct ataagatgtc atagaagtat cttggtggga aaagtattaa
      541 ataaagaagg ttttgatgta aaatatataa tgcatgaagg aaatttggca tatcaggaag
      601 atatagagga atcattattg gataagtatt ttagtgatag aaagcaactg agtattgaca
      661 atttattagg gagtgcatta acgagagaag aaatgataca agaaggttat aagctagcta
      721 ataaagaaat aggatataga actgagaaac taggtaaata aaaatataca aatagataaa
      781 atacagtact tatcttaagt aaaataatat tatgaattta taatagctag gcatgatgtg
      841 ccaattaaaa atattctagt tatttaataa tatcattaaa tttagggtat tttttaatag
      901 cacatcatgt tgcagtttta aaaaataaaa tctctaatat tgccataatc aactgttcta
      961 ttaagagtag gatgactaga tataaatcta actgtaattt ttgaatttaa caaacaagga
     1021 tttgaaatta aaaatactct atctccattg acaatagaag taatagctac tggcaaaata
     1081 tctacaaatg tatcatatct gtttttagca atttgataag gataatttcc tacaaaaaca
     1141 aatagattta aatcatatat attatcagta aaccaagccc attgaccata aactttgtca
     1201 atattcattg tgcctaaacc aagtgtatca aagtatgttg ctatagttaa ataaatattt
     1261 tccatgtttt ctaaatatga aacagtatat cttctgaaac gtatgggtgc tgtggaagaa
     1321 ataggataaa ccatatttac atttagatta tttgcattta aattaaccaa agtatcacat
     1381 cctaaaatta gtctataaaa tacaatattt aaatggatgc gattttgtta catctaaagt
     1441 gtaatatttt atatattgaa aaagtggtaa aagtttggat gattaaataa tattataaaa
     1501 aatatataaa atgtacttat tgttattgct acacgatagt gatacaatat taacgaaata
     1561 tagctacttt taataataac ggaaaatatt tatattaaat taataaatat tttaaatgtg
     1621 gtatccataa aaaataataa acataagata attataaaga tatactgtgt tcaaatgata
     1681 ttaaacaaga tttatattat ttgaaagcag tatattataa tgcgaacaat acaattctaa
     1741 actttgcatt gtcaatttac agttcgataa actaattaaa aattaaattt atataaagag
     1801 ggaaaagagg aatagggatg tttataagga aaaatgttat aaagttagca attcctataa
     1861 tgatagagca gacgtttgtc atgcttttag gagtatttaa tacaatgatg gcaggtcata
     1921 ttggggaaga agctgtatct gcagttggaa tggttgattc aataaacaat atatttatat
     1981 ctgtatttgc tgcactatct gttggagcta ctgttgttgt agctcaacaa ataggaaaaa
     2041 aagatagtga aaaggtgaat gaaactatta agcaggctct agtgtctgga gtaatattat
     2101 cctttacaat aacaatattg atgtgggttt ttagaactgg tgtaattaat ttattttatg
     2161 gttccgcaga agaattggta aaatcaaatg caaaattata catagagttt acattattta
     2221 cgtatccatt tattgcgaca caacaaatag caaatggtat tttaagaggg tgtggagatg
     2281 taaagaaacc tatgtatgtg actatgttta tgaatatagt aaatgtaaca ttgggatata
     2341 tacttatata tggtattgat ttaaatcctc taggaataaa tttgcaaaca ccatcctatg
     2401 gcataacagg tgcagctata tctattgcta tagctagaat aattggatgt atagtaatta
     2461 taagagcttt atttaaagga aacgaattta tatctatggg taaaatattt ccatttaaga
     2521 ttgatgttga aactcaaaag tctatattta atataggaat accagctgga gttgaacaac
     2581 tactttttaa tgcaggtaaa ttgattgttc aagtatttat agttactatg ggaacatcag
     2641 caattgcatc caatgcaata ggaatgtcaa ttgcatccat aataaatgta attgggaatg
     2701 ctttagcttt gtctgctaca accttggtgg gacagtatat aggacgtgga gatattaaag
     2761 gagctaagag tactttatta tatttaacta aatttgcaac aatttgttta tttatagtag
     2821 gagctatatt tataccaatt gcaccctgga tttcaagtat gtatactcaa aatgctagtg
     2881 taattgacat ctcaacacaa ttaattcgaa gtgattgttt agctatggta atatggccta
     2941 tatcctttgt attatcagca gggctaaagg gagccggtga tactagatat acaatgatga
     3001 ctgcagtaat aggtatgtgg atttttagaa tttttacagg ttacgtattg ggggttgtat
     3061 tagaatttgg agtgttagga atttggatag gtatgtatac tgattggctt gttagaggga
     3121 ctttatattg tttaagatta agaggaacaa agtggctaaa acacaaagtt gcatagaata
     3181 tatgaaatca cttttagatg gataactcta tttagaagtg attttttatg tagttattgt
     3241 cataattata agtatgatat ataaggtgat taaattacat aaataaaggc tataatagta
     3301 tggatattat aaattatata ttgagcagaa ttacataatt tggcgataaa tattagaaat
     3361 atatatctat agaaaataat atatatgggt atattattaa atataaaaaa tattaggtat
     3421 ttatttaata ataaaggtgg gtattgttat gataaataaa ataggaaaaa taactctcta
     3481 tgtaaataat caagaggatg caaagttatt ttggacagaa aaattgaact ttgtagtaac
     3541 ttatgaaaag gaaatgggtc caaatatgaa atgggtggaa gtagcaccaa gtaaaaatga
     3601 atttacaaca tttgtgttgt atgataaaaa tctaatgatg aagcaaaaca aagagataaa
     3661 tgtatctcat ccatcaataa ttttaagtac aagagatatt aatggaactt atgaacaaat
     3721 ggcaaataac gaggttgagg ttggggaatt aatggagatg ccatatggaa ggatgttttc
     3781 ttttaaagac caagatggaa atgattattt ggttagagag gataaataat tttacaaagt
     3841 attaaattta ctcaatatct gatataatta gagacaataa ttattataaa ctagtctagg
     3901 attagaatta tataattaaa aattaagtgt aaattttgag tagaggtgat aaaatgttta
     3961 agaaaatggc agtactaaaa gatatagcaa ctaaaatagg tcgtaaaaaa gcatatgaac
     4021 tattagaaat ggttgaaggt aatgatgcct ttgtagctga ggtaaagata aaaaagaatg
     4081 gaatagaatc taaaaaagaa gaaattatgt taaaagataa tcaaaaaata atattagagt
     4141 atatagaagg ttaaatttta aatacatata agttgatata ctaaatttct gagatgagtt
     4201 tacataaaac tcatcttttt tacttttcaa taactttgtt ttattattta atatacattt
     4261 tatctcctta gagttgctga tatataaaaa ttactgatat aataaaagaa tggatattaa
     4321 tgtaatattt tatagaaaag aggggtatta tgcttgaaat aaaaaatctt tcctttaatg
     4381 ttgagtctaa caatgaggaa ctaggaataa taaatgatgt tagcctttca tttgagaggg
     4441 gaaaacttat tgttatcaca ggtccaaatg gtggaggaaa atctacaatt gctaagctta
     4501 ttatgggaat tgaaaaagct acgagtggtc agataatttt agatggagaa gatataacaa
     4561 atcttagtat tacagaaaga gcaaaaaaag gaataggata cgcatttcaa caaccaccaa
     4621 gaataaaagg tatgacagtt gaaaatttac ttacacttgc tcatggaaaa ccattgtcta
     4681 ctgatgtatg ttgccaatac cttacagatg ttggattgtg ttcaaaagac tatttaaatc
     4741 gtgaagttga taactctctt tctggagggg aaatgaaaag aattgagata gcaactcttt
     4801 ttgctagaga tttaaaggtt tctatatttg atgaacctga agcagggata gacctttgga
     4861 gctttggaaa actcaatgaa agttttaaga aaatacatga agagtctaat cagactataa
     4921 tcataatttc tcaccaagaa agaatattag agttagctga tgaaataatt gttctacaag
     4981 atggttcagt aaaaagccat ggaacaaaag aggctatatt acctgaaatc atgtgtcaag
     5041 ttaattcaag ttgtgaatta atgaaagaca tgaattaatt agaaaggaag gcaaattaaa
     5101 tgaacaaaat agatttaaat cttttagaaa aagttgctga acttcgtgga actccattag
     5161 gagcatacaa tatacgtaaa aatgggcaag gagtagagag acattcaact gaaaatgtaa
     5221 ctataattcc taaaactgat aaacctggaa tagatattat agtaaagcca aatacaaaag
     5281 gagaaaatgt acatatacca gtaatagtaa cagaggctgg tgtacaggat atggtatata
     5341 atgattttga aattggagaa aatgcagatg ttgttataat tgctgggtgt ggtatacata
     5401 actgtggttg tgatgatagt gaacataatg gtatacatag atttttctta cgtaaaggag
     5461 ctcgcctaaa atatgtagaa aaacattatg gtgaaggtga tggagaagga aagagaattt
     5521 taaatccagt tactattgtc aatatggatg aggattcata ttgtgaaatg gaaacaattc
     5581 aaattaaggg tgtagattca actaaaagag aaatggatgc aaacctaggt aaaggagcta
     5641 aaatagttgt atttgagaaa ttattaactc atggtaatca agttgcagaa tctaatatga
     5701 aggttatctt agagggtgaa aactcttctg ctcaagtagt ttctcgtaca gttgggcaag
     5761 ataattctaa gcaaaagttt aatccttgtg ttattgggga ttccctttgt tctgcacatg
     5821 ttcaatgtga ttcaataatt atgggaaatt ctcaaattag tgctatacct gaaattgctg
     5881 caaatcatcc agatgctctt ttagtacatg aagcagctat tggaaagatt gcaggagacc
     5941 aaatactaaa gatgatgaca ttaggtctta ctaaagaaga agccgagcaa caaatactta
     6001 attgcttctt aaagtaaatt aaagatttta tttaatttaa tataattaat atagtataat
     6061 atagtgaata agtctaaaat atttttattt ttaggcttat tttctttatg taagaaaagc
     6121 tattagagaa attatattgt aattaggatg tggaataaat gaaaaattta cttataagaa
     6181 atgggacaat aatatctcca acagatggta tcaaatttga aaaaagagat atcttggttt
     6241 tacatgggaa aatatatgct attggattga accttaaaga gaaaataaga aaagaaaaat
     6301 tacttacaaa taaagagttc gagataataa atgccgataa tatgtatatt tccccaggat
     6361 ttatagatgt tcacacccat tgttataaga gaaaattacc tacaggtatg gattctgatt
     6421 caattggaat tgaaaaaggt tctacagtta tatttgatgc aggtacagct ggtccctcaa
     6481 actactatga ttttaaaaaa aagtatatgg atgagtgtaa aacagaagta tattcttttt
     6541 taaatgttac aaatgaggga ctaaatatat tgaatgaatc aacttattta gatgttataa
     6601 attttgacaa ggttgagtca attgtagaaa aaaatataga aaagataaag ggtataaaag
     6661 taaaagcgag taaatttcaa tctaatgaaa gtggtataga tataattaag aaatcaaagc
     6721 aagttgctaa aaaattaaat ttacccttag tggtatatat aggagagtat ccatcatata
     6781 tagatgaagt aatgaatatt ttgggaaaag gagatgtggt tacaccggta tatggaaata
     6841 gtacaaatgg aatccttaaa gaatctggca cattaagaaa atcagtaata aatgcaaaga
     6901 atagaggagt attgtttgat gtatgtcatg gagtggacca atttgatttt aaagttttta
     6961 aaaaagcaat aaagcaaggt ttagagcctg acctaataag tactgatatg catataaaaa
     7021 acatgaaaga cgtaaactat tctcttttaa atgttataaa taaaattatg gaacttggga
     7081 tatccctaga aaagtgtata gaaaaagtaa ctgaataccc aacaaagatt ttcgatttac
     7141 atggaaataa aggcagaata aagattggtg gagaaggtga ttttacaata tttacaatgg
     7201 aggagtgtaa tgatattata aaagactaca ataataatga acttgtttta aataaaaaat
     7261 taagattgca atatactgta aaatcatgga tgaaaagcag tgaagtttat agacatggat
     7321 acgaaaatac tataataaac taaacaatag tgataaaata ataaatatat gtttaaattg
     7381 gtatatattg gataaatatc taaggaaaca acaattaaaa aataataatt ttaggggagg
     7441 tattttagta tgaaagcatt tgttgataag gatgtatgtg tagcttgtgg agcatgtata
     7501 ggaacatgtc cagacgtttt tagtatgagc gatgaagggc attcagtagc aattgaagga
     7561 gaaataccag cagatttaca agatttatgt aaagaagccg aagaaggatg tccagtttca
     7621 gctataacag ttaaatagag ctgtttaaat ttatttttaa aataaatata taagatttaa
     7681 ttggtactta agctatagta ttagaaattt ctattactat agctttttaa aatagataaa
     7741 cttaatatat aagatattac tttttacaaa tatggaggac tgaaatatga aaaaaaatta
     7801 tgaaatagta ttattcgatt tagatggaac acttacagac cctaaagaag gaataacaaa
     7861 gtctattcag tattctttaa atagctttgg cataaaagaa gatttggaaa acttagacca
     7921 atttatagga ccaccattac atgatacttt caaggagtat tataaatttg aagataaaaa
     7981 agctaaagaa gctgttgaaa agtatagaga atattttgct gataaaggga tatttgaaaa
     8041 taaaatttat gagaatatga aagagatatt agaaatgctc tataaaaatg gtaaaatatt
     8101 attagtagca acttctaagc caacagtttt tgcagaaact atacttagat attttgatat
     8161 agatagatat tttaaatata tagcaggtag caatcttgat ggtacaagag tgaataaaaa
     8221 tgaagttata caatatgtac tagacctatg taatgtaaaa gataaagata aagttattat
     8281 ggttggagat agaaagtatg acataattgg agctaaaaaa ataggtatag attctattgg
     8341 agtattgtat gggtatggtt cttttgaaga gattagtgaa agtgaaccta cttatattgt
     8401 cgaaaatgta gagtctataa aagatatttt gttataaata tcttttatag acaaaaatat
     8461 tacaaaatgg tgtcgcatat ttaaaaatat gatattatag ttaaggaagt catttaaaaa
     8521 agaggaggat gcacttaaaa tgaaatctat tttaattatg ggtggaagcg acttcatagg
     8581 ttctgcactt gctaagcgct tgattaaatg tgggtatcaa attgatatac ttacaaatgg
     8641 taaaaaggaa atagattata atggttttaa agaacatcta atttgtgata gaaaagttcg
     8701 aaaagatatg gaaaacatta tcacaggaag aaaatatgat tacatatatg atatgacagc
     8761 atatacaaaa gaagatgtaa gtaatctgat tgattttata agcatggata acttaaaaaa
     8821 gtatatagta ttgtcagcag gagcagtata taaagatagt ggaagaaata taaaagaaga
     8881 aaatgagaaa ggtgaaaatg aaaactgggg aaaatatggg ttaaataaaa aagaggctga
     8941 agattttatt ataaatagcc ctataccata tataattatt agaccaactt acatatatgg
     9001 tgaaaataat aatttatata gagagtatta tttctttgaa aaaatagaaa aaaatgaaaa
     9061 gataccagta ccaaaaggaa aacaagttag taatcaattt atatatattg gggatttggt
     9121 aaaggtattg gaaagcataa tgaaaaatcc tcatgtaaga gaggcctata atgtgacaaa
     9181 tccacagtta atttcttggg atgaccttat atatacatgt ggagaaatta ttggcaaaga
     9241 acctataata aaatatgtag atatggaaaa agtagagttt agagaaagaa cgtatttccc
     9301 atttagaaat atagacttta atcttgatat aaataagctt attgaacatg ggctttatat
     9361 accaaacgtg ttactaaaag aaggtcttac agcaacatat aaatggtttt cagcaaataa
     9421 gccaaaaatg catgacagaa aaatgaacaa agttgagtct gtaatgcaaa tagtttaaaa
     9481 aaataaataa gtaggtagat attttgaact caaaaattaa gtatttttta aaatttttta
     9541 tcttaatatt tattttgatt tttatatact cagttgcgat agagccgagt ctattaattg
     9601 tgaaaaaata taatataagt gaaaatggag cattgataaa aaatgattca attgagagta
     9661 aatctgaaag tgtaaaagta gttcaaattt cagatactca aataggtagt ttttattcaa
     9721 ctaagaatct caaaaaagta gctaataaga ttaacacttt gaatccagat ataatagtat
     9781 ttactggtga tttaattgat tactccaata aaaatccaag tgtagatgaa attacaacta
     9841 ttttatcaag tatgaatgct aggttgggaa aattttcagt atttggcaat catgattata
     9901 tgtataaact tcctaggtat tataggcaga ttatgaaaaa ttccaacttc aatcttttag
     9961 taaatgaaaa taaaaaaata aaattaaaag atgataaata tattaatata ttaggtgttg
    10021 atgaaatatt aaatggaaat cctaatataa aatatctgga gagtcaaata gataataaaa
    10081 gttttaattt attgttagca catgaaccag atttagtaga tatgcttagt aaagatacaa
    10141 tgaatttagt tctatctggg catagtcatg gtggtcaaat aagattacct attaagggag
    10201 cattagttac tcctccatat ggaaggaaat atactaaagg gttttatgac ataaatggaa
    10261 atcatttata tgtgagttcg ggtcttggaa gtacaaagtt accatttaga ttttttaata
    10321 taccagagat aatagaattt aatattataa tctaacataa aatatttaga aaatataatt
    10381 ttgcttttta taatatgtag atgtatatac tgtaaaaatg gattaaacca gtagattgaa
    10441 tgaattaata gattaaatta atatataaaa tatttaagtt ataattaatg tattagacaa
    10501 aataaatact attaagaact attaaaatat agattaaaga taataacata taataaataa
    10561 aaaagtaaga ttgtgtagat tatataaaga ttatttacaa ttaatacaat cttgcttttt
    10621 tatttaaaaa ttttaattat tatttaaaat tttttatctt ctctttatcc catataaaca
    10681 atttctcaga attatttaaa gcaccaaata catttctttt aaaatcaggc atctctttat
    10741 ttaaattagc aataagattt tttatgtact ctctcctagt atgaccattt gcagggcagg
    10801 gatttgtgat aataggatag ttagaatctt ttgaaatttt cttagtcata tactcttcta
    10861 tgtaaaccat aggtcttata actgttaaat cctgtctgtc taggtatgtc tttggggaaa
    10921 aacaatttac tctaccctca taaaacatag acattaaaaa tgtctcaatt gcatcatctt
    10981 tatgatgacc aagagctact ttattacatc caagtttttt agcattatcg ttcaaagcac
    11041 ctcttcttaa attagcacac aatgcacaag gattcttttc ttgtcttata tcaaatacaa
    11101 tttcttttat gtcagtttga aattcataaa aaggaacatt tatttcttta cacaatttat
    11161 ctagtggaga attatcaaca cctccagtat ttaatgttat ggctatcaat tcaaagtttt
    11221 gtggagaaaa tctttgataa cttttaagta tatgaagtag agttaaacta tcttttccac
    11281 cagaaagtcc aaccgcaatt ttatcatttt cttgtatcat atcaaaatct tgtatagcct
    11341 tacgagcttt acttagaagt ttttgtatag acattttatc accatccttt aaaaatatta
    11401 aatttaagat ttatttaaga tttacttata aaatacttaa gtcttatact ctaaaattaa
    11461 cattatagca aacataacaa aaaggagaag atttaattat gaaaaaattt aaattaatag
    11521 gagccattat tttagtagta tttataagtg tatttatatc taaagataga ttacttaaga
    11581 aagataaaga tccaatagta gctgctcaac atgattcaga acaagatgtt gaggcaaatt
    11641 ctcaaaataa aaatcactca aatagtatat caaatatatt attggtaaac aagacaaatg
    11701 gaattagcaa aaactatact cctgaaaata ttactaaggt aaacatacct tttgttgaag
    11761 aagcaacaga ggaagaaaaa caaatggcag gagaacctgc aaaggcagtt gaagatttag
    11821 ttaagcaagc taattcagaa ggaatacagt ttttagggtc ttcagcatac agatcttatg
    11881 atactcaact ggatacttat acaagaaggg taaaatctca ggggcgagaa aaggcagatg
    11941 cgtatgtagc aaagcctggt tacagtgagc accaaactgg actttgtatt gatttaacaa
    12001 atccagaaag atggtttgtt ggaagtacaa aagaagctaa atggttggca gaaaatgctc
    12061 acaagtttgg atttataata agatatcctg aaggtaaaga agatataaca ggtacagcat
    12121 atgaaccatg gcatattaga tatgttggta aggatgctgc cgaggaaatt tattcaaaag
    12181 gattaacttt agaagaatat ttacaaaata aataatataa atgtgtaaat aatttgtatt
    12241 atattggaaa tttagaaatt aataaattta tagattacac taactaaaat atagtataat
    12301 tttatatgaa taaagctata tacaaagatt aagttaggat gtgtaaaaat gaataataca
    12361 gttcttgttg tggatgatga aaaggaaata agagacctta tagagatata tcttttaaat
    12421 agtggatata atgtaattaa agctgaagat ggaaaagagg ctctagatat tttaaaaaag
    12481 gaaaatattc atttaatgat attggatata atgatgccta aaatggatgg gatagaagtt
    12541 tgtagaaaag ttagagaaag tttgaatata ccaatactta tgttaagtgc aaaatcagaa
    12601 gacatggata aaatacaagg gataatgact ggtgcagatg attatttgac aaaaccattt
    12661 aacccacttg aacttactgt gagagtgaag tcgctgatta gaagagcata ttattttagt
    12721 ggtgctaata atgaagataa agatgtcata aaggttggag tagtaattat agataaggaa
    12781 aaacatagtg taacagtggc tggaaatgaa ataattttga catcaagaga atttgatata
    12841 ttatatttac tagcaaataa taaaggcaga gtatatagta cagaagaaat atttgaaaag
    12901 gtatggaaag agaaatatta ccaatctaat aatactgtaa tggttcatat gagcagaata
    12961 agggataaaa ttgaaaaata tacagatgga gaaaaaataa ttcatactgt atggggagta
    13021 ggatataaaa ttgaaaaata aaagtttatt aaatcatatg tttcgaccca tattagatct
    13081 tataataagc tgtgctttaa cttcaatgct tattttaaca agtatgtata taataaaatc
    13141 attatatgtc aatagtgata tgtctgtagt taatcaactt gtatatcaag caagagattt
    13201 cagaagtctt atagggtatg atttatttta tccattttta tttacaatca catcattagc
    13261 aatttattcc atgatatcgt acaagagaaa taagcaaatt gtagatttta taagaaagac
    13321 taatagaaaa aatcaaaaat tagaaatcat taaaagtgct gtaaatttaa tggatgaagg
    13381 taacttagaa aaaagtatag atattgagta tgatttagat atattaaagg ataaagaaaa
    13441 ggatgatatt gatgaactag cacataaaat caataatata gtccatcaac ttagaaatat
    13501 aactattgaa gagagacaag ctcagcagac aaaaacagat ttaataacaa atgtgtcaca
    13561 tgacttaaga acacctttga catccataat gggttattta ggagtgatag aagaaggtaa
    13621 gtataaagat gaagttcaga tgatgtatta tgttgatatt gcatatgaaa aatcgaaaaa
    13681 tttaaatgtt cttataaatg atttgtttga attgactaaa atgcaaaata atacaataaa
    13741 attaaacaaa atagaaataa atttagtaga gttattaagt caagtagtat ctcaatttga
    13801 aatttatttt aaacaagaat ttatggttag tagaattaat tttaatgaag aaaaattaat
    13861 tgtaaaagct gatccaaata aattggtaag agcatttgaa aatcttataa ctaatgctgt
    13921 gaaatatgga aaagatgggc attatgtaga tatagtcaca gagaaaaagg aagatatggc
    13981 ggttgtaaaa gtgataaatt atggggaacc aattccagtg ttagatttac caaatatatt
    14041 tgatagattt tatagagttg aaaaatcgag aaatagaaat gttggaggtt ctggattggg
    14101 tcttgcgatt acaaaaaata ttgtaaattt acatgatgga gaaattactg tttcaagcga
    14161 taaatatcaa actgtgtttg aagtacaact gccaataata caatcagctt agttataaaa
    14221 taaaagtacg attatgtgat ataaatatta taacaataaa gtgtttacaa aaataaaaaa
    14281 gtaatatata atttaagtca gtttaataag atacttatca agagttaggg gagagagtta
    14341 gctcagtgat cctataccaa ccttttatca ggtggtcctg ctaacaacga tgagatgact
    14401 tgaaataata tactcttctt atcaataata gataggaaga gtttttttta gaattaatgt
    14461 aacaaatttt aaagtataat ggaggattaa ataatggaaa aagtagaaag ttttaagcta
    14521 gaccatacaa aagtaaaagc tccttttgta agaaagtgta gtgtacttga tggagtaaag
    14581 ggagataaag taactaaatt tgacttgaga tttttacagc caaatgtaga aagttttgga
    14641 actgctgcta tgcacggttt agagcatctt ttagctactt atttaagaga tacattagat
    14701 ggtgtaatag acttatctcc aatgggatgt agaacaggat tttatttaat actatgggga
    14761 gatgtagatg ccaaaacagt taagattggt ttagaagaag cattaaaaaa agttcttgaa
    14821 tcagataaaa tgccagcagc tacagctata gaatgtggaa actacagaga cttatcttta
    14881 tttggggcaa aggaatatgc aaaagatgtt ttagataaag gattttcttt aaatatatat
    14941 ggagaataat ttaattggaa gagaagatat aagcataaat caaagtgaag ttttaagata
    15001 tttacaatat agaggtcaag agatagataa agaccttaaa tatactattg atgaatgtgt
    15061 agctataact aaaaataaaa taaatcctag atatttatct aggatttatc ctattaaaat
    15121 aaataaagat aatgttgtag aattagaggg aacaaattta acattagaga gtaaagatgt
    15181 atatgaactt ttaaaagaat gtaatgagtg tgtaattatg gcagctacta tagggataga
    15241 tatagaaaaa gaaataagac gacactctta ttcaaattta acaaaaggac taataattga
    15301 ttcatgtgga acaacagcaa tagaagaagt gtgtgacatt gttcaaaata atttagaaaa
    15361 ggttttttta aagcaagata aatttataac tatgcgatat agcccaggat atggagattt
    15421 accaatagaa aaaaatatag atatattaaa cgttttgaat gctcaaaaac aaataggtct
    15481 aacaattact tctaatggaa ttatgatacc gagaaagtca gtagtggcta ttataggtat
    15541 ttctaataca agaataaata aatctaagaa agaaaagtcg tgtgaaaatt gtaaaaacta
    15601 tagaaactgt acatatagaa aaggagcgaa ctcttgtgga aataagacaa tatctgaaaa
    15661 ataatatatt aatatttgat ggtgcaatgg gaacaatgct tcaacaaaaa gggttaaagt
    15721 taggtgaaaa tccagaagta tttggtcttc aaaatccaga taaacttata gaaatacata
    15781 cagcatactt agaggcaggt tcgaatgtaa ttcttacaaa tacttttgga tgtaatgaac
    15841 taaaattaga tagtaagtat actgtagaag aagtgattga caatgctgtt ttggtggcta
    15901 gaaaagctat agaaaatgta gataatacta aaccaagata tgtagcttta gatattggtc
    15961 ctataggaga aatgctagag cctatgggta ctttaagttt tgataaagca tatgaaatat
    16021 ttaaaagaca agtactacaa ggcgttaaat ctggagttga tgtaatagta atagagacta
    16081 tgatggattt atatgaagct aaagtagctg tattagcagc aaaagaaaac tctgatttac
    16141 ctattttctg tactatgaca tttgatgaag gtggaagaag ttttactgga tgtatgccag
    16201 aatgtatggt ggctactata gaaggtctag gtgtagatgc aataggagtt aattgttcat
    16261 taggacctaa acaactttta ccaatagttg aaaaaatagc atcaagagct acagtccctg
    16321 ttatggttca agcaaatgca ggtcttccaa atatagttga tggagaggct atatatgatg
    16381 ttgatgcaaa agaatttttt gaaggggtta aaaagttcgt tgaagttggt gctactataa
    16441 taggtggatg ttgtggtacg aatccaagtt ttataaaaga aatatctgag aatataaatt
    16501 ctgttacaaa gggatgtatc gagaaaattg ataaatgtgt agtctgttca ccatcaaaat
    16561 ttgtggaagt acaatcgcct acagttgttg gagaaagatt gaatcccaca ggtagaaaat
    16621 ctctacaaga agcattaaaa aatgaaaatg ttgattatgc tataaattta ggtttagaac
    16681 aagtaaatgc tggagcacaa atacttggag taaatgttgg tcttccagag attgatgaaa
    16741 aaaaactaat gccaaaatta ataagagaga ttcaagcagt agtagataca cctttacaag
    16801 ttgattcttc caatgtagag gccttagagc aaggacttag atattataat ggtagaacaa
    16861 tagtaaactc tgtaaatggc aaagaggagt ctttagaaag tatattacca atagttaaaa
    16921 agtatggttc atgtgtggtt ggacttacac ttgatgaaaa ggggatacct aaaaaggctg
    16981 aagaaagatt tgaaatagca aaaaggatag ttaatagagc agtttcttat gggataaaac
    17041 caaaagatat atttatagac tgtttatcat taactgtttc agcacaacaa gaagaagcaa
    17101 tagaaactat aaaagctata acaatggtaa agacactagg agtaaaaact atattaggtg
    17161 tttcaaatat ttcatttggt cttccaaata ggaaagcact taatgcttct tttctaacat
    17221 tagctttagg ggcagggctt gatttagcaa ttataaatcc aaatgaatat agtatgatgg
    17281 aagctatcaa ttcttttaaa atattaaata acacagataa aggatgtata aactatataa
    17341 atcaatatag caatataaat aattcaaaaa aatcggattc atctacaaag attgataaag
    17401 atttgccctt ggatatttta gttgaaagag gattaaaaga tgaagcaaaa aatgtaacac
    17461 tgaatctttt aaaagagaag gatgaaaatt atatattaga tgagatttta attcctgctc
    17521 ttgataaagt aggaaaaaga tatgatagtg gagatatatt tttaccacaa ttgattcaat
    17581 cagcagaaac ggtaaaagtt tcattaaata caataaagga aactttatta agtaaaagta
    17641 gcaataatgt atccaaagga aaaataatag ttgctacagt aaaaggtgat atccatgata
    17701 ttgggaaaaa tatagtaaaa ataatgctag aaaattatgg atatgaagtt atagatttag
    17761 gtaaagatgt ccctattgaa gaagtagtgg aagttgctaa aaagagaaat ataaaattag
    17821 taggattaag tgcacttatg actacgactg ttcaaagtat gaaagataca attcaggcac
    17881 tgagagataa tgagataaat gcaaaggtat ttgtaggagg tgcagttttg acagaagaat
    17941 acgctgaaga aatgggtgct gattattatt caaaggatgc aaagtctgct gttgaaatag
    18001 caaagttaaa tttttagcat ataaattaaa ataaaggttt tactaaatag gatattttat
    18061 atataatata aatataagtt ttagatactt atgtttaaaa caataggaat taatctataa
    18121 taatgagatt aattaatata aaaaatattt atcatcaaca ttaaaaaggg ctgggaagtc
    18181 tggtataact tccatcagcc ctttttagtt aaaatcatat gttaaaaagc ccaattacca
    18241 tttttaaaaa tctgtatttg ctttccatca taagtctcac caataatatc catatcagca
    18301 gaaccaatca taaaatctac atgtgttaaa ctgtcattta caccatgttc atccaattct
    18361 tcatctttta aattatttcc accttccaga caagtcttat aagcagaacc aattgcaaag
    18421 tggcaagaag cattttcatc atatagagta ttataaaaaa ttatatccgt attggatatt
    18481 ggtgagttgt atggaacaag agcaacttct cctaaataat gagagccttc atccgtttca
    18541 ataagttttt ctagtgtatc tagtcccttt tcagcagaga aatctattat tttaccatct
    18601 ttaaatgtta agaagaaatt gtcaattatg tttccaccat aaacaagagg ttttgtacta
    18661 taaacagttc catttacttt aaacttatgt ggcattccaa aaatctcttc agttggaata
    18721 ttcggattaa aacttattcc attagggtct ttggatgctc cacttagcca aacatgaccc
    18781 tttggcaagt ctagagtcaa atcagttttt gaggatttat attttaaagt tttaaagtta
    18841 tttttattta gaaagtccat tttagattta agattttcat tatgttcttc ccatgctttt
    18901 atagggtcct gaccatctac tcttgaacat ttaaatatag catcccacag tttttctatt
    18961 gctttatctt ctgataaatt tggaaacact ttttttgccc aatcttttgt tggcacagag
    19021 actatactcc atttacattt atcagttaga gtgtaagaac gccattcttt taaagctttt
    19081 ccagatgcct tctgaaaatt tgcaactctt tcagggtcaa catcttttaa taaatctgga
    19141 ttttgggcat agactgataa aaatgcccca ccttcttttg ctatatctac atattgttct
    19201 gctgtccatt tggggaactc atgaaatgct tcatctggag catataagta tttaataaga
    19261 gtactttctt catcagacca ctctatataa acatttttag caccacattt atatgcttct
    19321 ttaagtgcat ttcttgcaaa atcagcacat tctattggtg actttataag tagaatttga
    19381 cctggctgta tgttaactcc aacttttatt gctaattcag cgtatttttc taaatttcta
    19441 tttaaattca tatttatcct cctatatttt taatattgaa taacttataa agtattataa
    19501 cattacttat gtttttaata ttgaacaact tataaaatat tataacttta cttagatttt
    19561 gggtcatatt caaaatattt atactttata gattgagaaa taaaaaaata ttttaattaa
    19621 aacagattat gaggataaca aacttattta agcagtatgt tttttgttga taataatatt
    19681 aaagtagaaa taaaaatact tttttagtga aaagctatac aagaataccg aaaaaaactt
    19741 ataatataat aatgagatag atatttataa atgaaaaaaa atttttctgg caaagcttaa
    19801 ttaaaaaggt aatagctagt gttgttattt aaattagcat agaaaatcaa ataaaaaaat
    19861 tatgctaatg tatagacatt gataaggtag aatttaagcc ttttgaatgc tatattaaac
    19921 tagagtagat attcttatga atttcaaaat agggcaggag gaaaaaggaa ataaaactat
    19981 aaaaaatata aagtatttat ataaatttat agagtttata cgtcagaaga tttcagttag
    20041 gagggcataa atggagcttt atgaagccat tttttataga aaatctgtaa gaaatttttc
    20101 cagtaaaaag ataaaaaagc cattgatgga agaaataaaa agaagttgta aaaatttaga
    20161 atacttaaat gaagatttaa atattaaagc acatgttgtt gatagaggtc atataataca
    20221 ttttttgatg ggtaaggaat gtaaagtaaa ggcacctcat tatatagtag ttacatcaga
    20281 caaaggtgag gactatttac agaatatagg atttgctaca gaaggtgtag ttttgcgcct
    20341 tacaacattg ggaatagcta cttgctggtt ggaaggaaac ttaaagagag aagatatctt
    20401 ggaatttgta gatattggga ataagaattt tgaagaagat gaggaagatg atttagatta
    20461 tctagctgga ataaaaacag aagaagatga tgaaaacaaa atagaaaatc cttatataat
    20521 aatagcattt ggatatccag caaaaaatga agagttgttt aggacgagat atgctagacc
    20581 tgatagaaag ccagtaaaag atatgataaa taagataaat aggaatagaa agtggaaaga
    20641 tatcttagaa ttgacaagaa gagctccatc tgtaaaaaat tctcaaccgt ggtatttcca
    20701 taaggatgaa cgaggtcttc atttatttga aaagagacct aagaaacact gtgaagatat
    20761 gaataaggtt agcttaggtg tagccttaag acattttgat atagcttgta taaaaaataa
    20821 aatagatgtt tcatatgaaa aacttcctat aaggaataaa ataggaaaat catattttat
    20881 aactgtagtc gagcatgtga aaccagaaga agaaacccaa gaagaaaatg taactctaga
    20941 aaaagaagaa tcgcaagatg aataaaattt tggaagggga tattggatat gtataagtgt
    21001 ggaaaatgta atcttagagg ctgttctaat ggaaatatgg ataaattaat gaagatatgt
    21061 ccaactagag aaattgaact acaacaaaaa gcaaagcaac tttatgaaga agaagagaat
    21121 agaaaaatag cttataatgc tggattagtt gaagcagggg ggtattgtca atatactaga
    21181 ctaaaagaga ttatagagtt tgctaaaaag tgtggttata aaaagattgg catagcattt
    21241 tgtattggac taaaaagaga aatgcaaaca gtgcaaaaaa tatttgaaca tcatgggttt
    21301 gaagtatttt ctgttatatg taaaaatggg gcaatgccta agagtacaat tggactagaa
    21361 gcatctcaaa caatatcaaa gtgttcagat gaggtaatgt gcaatccaat aggacaagct
    21421 ttatttttaa atgaagctaa ggttgacctt aatgtaattc ttggattatg tgttggtcac
    21481 gatactttag ccatgaaata tatggaagct cctataacaa ctttagttgt caaagataga
    21541 gtaactgggc ataatccagt tgctgcaata tataatgcag aatcatattt tgagaaaaaa
    21601 ttgtttgata aagatgaaga ataattcaaa gacttattga cataaaaagc aattagtggt
    21661 ttaattataa aaaagataaa actttaaatt atcctataga tacataaagt tatataaata
    21721 aagaaaaata ttacagctaa attccttaaa gtagtccaga gagactaaag ggttatagat
    21781 aatgttagta tataacctct agtaaatctt ctagaggttt ttgatgagta taaaataaaa
    21841 atttaaatat aataaagtct tttaaactag tccagagaga ctgggaaaga tatctcatag
    21901 ctaatgaata gagttatgta tagataaaat ccttatgcct cttttggtgt gaggattttt
    21961 ttatgattat ttaggcttta ttaaaaagga gatgtaaaaa tgataaatgg taaagaaaaa
    22021 ttgatagttg caattgatac tgacgaattt gataaagcta aagaactaat agacagatta
    22081 gaagatagtg tagacatatt taaggtaggg ctagagcaat acgttgccac aaaaggtaaa
    22141 acaatagatt atcttaaaga aaaagggaaa aaaatatttt tagaccttaa attccatgat
    22201 ataccaaata ctatgaaaag tgcagtgaaa gctgcagtga gagataatgt ttggttaatg
    22261 acaatacatg tatcagatat ggaaggaatg agacaatgtg cactaattgc tagggaagaa
    22321 gcagaaagat taaatataga aaagccatta gtagttggag taacagtttt aacttcatta
    22381 agcaaccaag atttacagga cataggatgt aatatgacaa cagaagagtt agctataaag
    22441 agagctaaac tggcaaaaga atcaggaata gatggggttg tttgttcagc gcaagaagta
    22501 gataaaatag tagaagcatg tggaactgat tttataacag tttgcccagg aataagacct
    22561 aaatcagcag aggttggaga ccaaaaaaga gttgtaactc catcagatgc aataaaaaaa
    22621 ggtgctcatt atttagttgt tggaagacct ataacaaaag cagaaaatcc aagagaatca
    22681 gctattaata ttgtaaaaga aatagaaaat gcttagataa aatatttggg aggtagctta
    22741 tatgaagggg aaattaattt tagaagatgg aacagtattt gaaggaaaag catttggata
    22801 tctaaaggaa gctattggag aagttgtatt taatacttca atggctgggt atggagaact
    22861 attgacagat gcaacttatt atggacaaat agttactatg acttatccac ttataggaaa
    22921 ctatggaata aatttagaag atgttgaatc aaataaggtg caagtaaaag gacttattgt
    22981 aagagaaaaa agtgataacc caaataattt tagatgtgaa atggatatag acacatatct
    23041 aaagcaaaat aaggtaatag gacttgaagg aatagataca agagcattaa ctaaaattat
    23101 tagaaacaat ggaactaaga gaggaattat aactttagaa aatgtatcat tacaagatgt
    23161 tcagtctaaa ttagaagagc attgtaacaa agattcagta aaaaatgtta caagaaaaga
    23221 aattgaaaat ataaaaggta ctggagctaa agttgtagtt atagactttg gtgttaaaaa
    23281 tagtgtacta gagtctctta aggcttgtaa ctgtgatata actatagtac cagcaatgac
    23341 tacagtagaa gaaattttaa atataaatcc tgacttaatc ttattatcta atggcccagg
    23401 aaatccagag gatataaatg atatagttga aaatataaaa aaattaatag gcaaaaagcc
    23461 attgacagga ataggtttag gacatcaaat tttagcattg gcattaggag gaaagacttc
    23521 aaagcttgta tttggacata gaggaggtaa tcacccagtt aaatgtttag aaactggaaa
    23581 ggtatttata acttctcaaa atcatggata taatgtttca gaaatgccag aaaatacaga
    23641 agtaactcac ataaatttaa atgacaatac tatagaagga atgagacata aggaattacc
    23701 aatatatagt gttcagtaca ttccagaata cacaacaaaa gaagatttag attgtatatt
    23761 taataaattc ttagcattaa tatagattta gtattattta cagatagtaa aaaccattta
    23821 gataaggaga aaaatttatg cctaaattag atagtataaa gaaaacttta gtattagggt
    23881 caggaccaat aataataggt caagctgcag agtttgacta ttcaggaaca caagcttgcc
    23941 agtctttgaa agaggaaggg attgaagttg tattaataaa tagtaatcca gccactataa
    24001 tgactgataa agaagtggca gataaaatat atatagaacc attaacaata gaatttatag
    24061 aaaaaataat agaaaaagaa agaccagaca gtttacttgc aggtatgggt ggacaaacag
    24121 ggctaaacct agcagttgaa ctttatgaaa aaggcatact agataaatat ggagtaaaga
    24181 taataggtac ttcagtagaa tctatcaaaa aaggtgaaga tagagatata tttagagaag
    24241 taatgaaaga aataaatcag cctgtaatag ttagtgatat agttacagat ttacaagcag
    24301 gtcttgatta tgcacttaca attggttatc cagtagttgt aagaccagca tacacactag
    24361 gtggaactgg tgggggtata gctgataatg aggaagaatt aagagaaata ctttcacatg
    24421 gtctacaatt aagtcctgtt ggacaagtat taattgaaaa gagtataaaa ggttggaaag
    24481 aaatagaata tgaggtaata agagattcaa agggaaattg tatagttgta tgtaatatgg
    24541 aaaatataga cccagtcgga gttcatacag gagacagtat agttgttgct ccaactcaaa
    24601 ctttaagtaa caaagaatgt gcaatgctta aaaaagcatc tttagatata ttaaatgcag
    24661 tagaagttca aggtggttgt aacgttcaat ttgcacttaa tccacatagt tttgaatatg
    24721 cagttataga gataaatcca agagttagta gatcatcagc tttagcatca aaagctacag
    24781 gatatccaat agctaaagtt tcgtcaaaaa tagctttagg ttatacttta gatgagatag
    24841 aaaatgcagt aactaaaaaa acttatgctt gttttgaacc aactatagat tatgtggtgg
    24901 caaaaatacc aaaatggcct tttgataaat ttaaaaaagc aaatagaaaa ttaggaacta
    24961 agatgatggc aactggtgaa attatgagta taggaagtaa ttttgaggct gctattctta
    25021 aagggataag atcacttgaa acaggaaaat attcattagt tcacacacca tctgaggaga
    25081 gaagcattga ggaattaaag aaaagagttg tagtacctga tgatgagaga ctatttgatt
    25141 tagctgaaat gataagaaga ggctataaag tagaaatgat agaacaaatt acaggtatag
    25201 ataaatggtt tataaataaa tttaaatgga tagtagagca agaagaaaaa ttaaaagtta
    25261 tgaaaattga agatttaact aaagactatc ttcttgaatt aaagaaaaag ggattctctg
    25321 ataaaggtat atctgaccta atgaagataa gtccagaaaa attatatgaa ttgagaagtc
    25381 tatacaatat aaaaccagca tataaaatgg ttgatacttg tgcaggtgag atagatgcta
    25441 tatctcctta ctattattca acttatgagc aatatgatga ggtagttgtt agtgataaga
    25501 aaaaggtgat agtattaggt tctggtccaa taagaatagg tcaaggtata gaatttgact
    25561 attgttcagt tcactgtgta aaatctttaa gaaaaatgga catagaaact ataatcataa
    25621 acaataaccc tgaaactgta agtacagact ttgatacatc agataagctg tattttgaac
    25681 cattaacaga agaagaagtt ttaagtataa tagaaaaaga aaagccagaa ggtgtaatac
    25741 tacagtttgg tggacaaaca gctataaaat tggctaagtt tttacatgaa aaaaatatac
    25801 ctatactggg aacagatttt gatgatatag atgcagcaga agatagagaa aaatttgatg
    25861 atttactaga gagactagat ataaatagac caaaaggaaa gggtgtatgg tcacttaacg
    25921 aaggtattga agttgctaat gagttaggtt atccagtatt agttagacca tcttatgtac
    25981 ttggtggtca aggtatggaa ataacttata acgaagaaaa attatctcaa tacctacaag
    26041 atgcatttga tagagaccat aaaaatccag tacttataga taaatatcta actggtagag
    26101 aaatagaagt agatgctata tgtgataagg aagatatatt gatacctggt ataatggaac
    26161 atttagaaag agctggagtt cactctggag atagtacaac aatgtatcca agtcaaaata
    26221 tatctgatga aataaaagaa aagatagtag aatacactaa aaaaatagct ttagacctta
    26281 atgtgcttgg tatggtcaat atacagttta tagaatttca aaatgaatta tatataattg
    26341 aggttaaccc tagagctagt agaacagttc cgtatataag taaagtaagt ggagtaccaa
    26401 tagttgactt ggctactaag tgtatgttag gagctaaatt aaaggattta ggatatggta
    26461 caggagttta caaagagcca aaattagtat cagtaaaagt accagtattc tcaatgtcta
    26521 aattatctaa agttgaagta agtctaggac ctgaaatgaa gtctacagga gaagtcctag
    26581 gtgtaggaga aaatttagaa gaagccttat ataaagggtt cttggcagca ggtagacata
    26641 tgtctgatga aagaggagta gtacttgcta ctgtaaataa tcatgataaa gatgagttta
    26701 tagagatagc aaaagatatg aaagaattag gatatacttt tgttgctaca gaaggaacag
    26761 ctaatagctt aagagaaaat ggaatagagg ctgatatagt aaatagagta gaagaatcta
    26821 gaccaaacat attagatgct ataagaaata aacaagttga tatagtaata aatacaccaa
    26881 ctaaaggaaa tgactctaca agagatggat ttaagataag aagaacagct attgaatttt
    26941 ctactgaaat aatgacatct ttggatacac taaaagcttt agtagaagtt aagaagaaac
    27001 atttaaataa agatgagtta aaagtttaca atatagctga atagaaatta agttgattgt
    27061 atagtttaag aatttaatta ttaaataaaa aaaagaggtt attttttcta ggagttttta
    27121 cttttgagat aacttctttt ttttaagaaa tttgtagtat aatagaaaaa gataaaaaat
    27181 aaatgtaaat aagtataaaa ctagaatgta aaagtgaaaa atagatatag atatttaaaa
    27241 gcttatattt agttaatgtt ctttagtttg tctaaagaga attataggag gtactgtagt
    27301 atgaaagcaa ggttaatatt agaagatgga acagttttta taggaaaggc atttggatat
    27361 ttagaagaaa gtgtaggaga agtagtattt aatacttcga tgataggata tggtgaagtt
    27421 ttaacagacc cttcttacta tggtcaaata gttactatga cttatcctct tgtaggaaat
    27481 tatggtataa atttatcatc agcagagtct gaaaaagttc aagtaaaggg attcatagta
    27541 agggagaaaa gtgattctcc aagtaatttt agatgtgaaa ttgatataga ccaatattta
    27601 aagcaaaata aagttatcgg acttgaagga atagatacaa gagctttgac taagatactt
    27661 agaaataatg gaaccatgaa aggtataatt acattagaag acagtaaatt agaagatgtt
    27721 aaacataaat tggataagtt ttcaaataca gaagcagtta gaactgttac aagaaaagaa
    27781 gtagagcata taaagggaaa tggtccaaaa gtggctgtta tggattttgg agtaaaaaga
    27841 aatatattaa gatcatttat agctcgtgga tgtgatataa caatatttcc agctacaaca
    27901 tctccagaag atgtattgag tataaaccct gacttaatat tcttgtcaaa tggacctgga
    27961 gacccagaag acctagaaga tgttatagaa aatataaaag ctcttattgg taaaaaacct
    28021 atagttggaa tatgcttagg acatcaactt ttagctttag ctcttggagg aaaaactgct
    28081 aaacttaaat ttgggcatag aggtggaaat catccagtta aagatttaga agaaggaaaa
    28141 gtatttataa cttctcaaaa tcatggttat tatgtttcag aagttcctga acaaatgaaa
    28201 gtgacacata taaatttaaa tgataatact gttgaaggaa tgagacatga gaaactagat
    28261 gtatacagtg ttcaatacca tcctgaagct tgcccaggac caaaagataa tgactatatt
    28321 tttgataaat ttttagaatt agtaaaataa ataacattag aatagctaac taaaaaacaa
    28381 gaataactta aatcaaaaag tttggtttaa ttaacataga tgtattaaat agatgtattg
    28441 atgagtaaat ttgaatttaa gataaaataa agtaattaaa gttgattgta tagatgatga
    28501 agataagaac tgtgaaaatt gtttagataa ggagaaagag ttatgcctaa attagatagt
    28561 ataaagaaaa ctttagtatt agggtcaggg ccaataataa taggtcaagc tgcagagttt
    28621 gactattcag gaacacaggc ctgccaagca ttaaaagaag agggaattga agttgtatta
    28681 gtaaatagta acccagctac tataatgact gataaagaaa ttgcagataa aatatatata
    28741 gaaccattaa caatagaatt tatagaaaaa ataatagaaa aagaaaaacc agatagttta
    28801 cttgcaggta tgggtggtca gacaggatta aacttagctg tagaattaca tgatgctggt
    28861 atattagata aatataatgt aaaagttata ggaacatcta tagaatctat aaaaaagggc
    28921 gaagatagag atttatttag agaagtaatg aaagaaataa atcaaccagt aatagttagt
    28981 gatatagtta caaatttaga agcaggtctt gagtttgcca ataagatagg atatccagta
    29041 gttgtaagac cagcatatac gctaggtgga actggtggag gtatagctga tactgaggaa
    29101 gaattaagag aaatactttc acatggacta cagctaagtc ctgttggaca agttcttctt
    29161 gaaaagagta taaaaggttg gaaagaaata gaatacgaag taatgaggga tggaaatgga
    29221 aactgtataa ctgtatgtaa tatggaaaat gtagacccag ttggagttca tacgggagac
    29281 agtatagttg ttgctccaag tcaaactcta agtgatgaag agtatcaact acttagaaag
    29341 gcatcaatag acataataaa tgcaatagag gttcaaggtg gatgtaacgt tcaaatagct
    29401 ttaaacccac atagtttaga atatgcgata atagaaataa atccaagagt tagtaggtca
    29461 tcagccctag catcaaaagc tacaggatac ccaatagcaa aagtagcagc taagatagct
    29521 ttaggttata ctttggatga gatagaaaat gcagtaacta aaaaaactta tgcttgtttt
    29581 gagccaacat tagattatgt ggtagtaaaa ataccaaagt ggccatttga taaatttaga
    29641 catgccaata gaaaattagg aactaagatg atggcaactg gtgaaatcat gagtatagga
    29701 agtaactttg aggctgctat tcttaaaggt ataagatcac ttgaaacagg aaaatattca
    29761 ttggttcatg ctccatcaga aggtagaaca ttagaagaat taaaggctag agtagtagta
    29821 cctgatgatg agagactatt tgacttagct gaaatgataa gaagaggcta taaagtagaa
    29881 atgatatcag aaataactgg tgttgataag tggtttataa ataaatttaa atggatagta
    29941 gaacaagaag aaaagttaaa agttctaaaa atagaagact tagataaaga atatttacat
    30001 gaattaaaga aaaagggatt ttctgataaa ggtatatctg acttaatgaa gataagccca
    30061 gaaaaattat atgaattgag aagtttatat aatatacaac cagtttataa aatggttgat
    30121 acatgtggag gagaatttga agctctatca ccttattact attcaactta tgaacaatat
    30181 gatgaggtag tagttagtga taagagaaaa gttgtagtat tgggttctgg tccaataaga
    30241 ataggtcaag gaatagaatt tgactattgt tcagttcact gtgtaaaatc tttaagaaaa
    30301 atgggaatag aaactataat tgtaaacaat aaccctgaaa ctgtaagtac ggattttgat
    30361 acatctgata agttgtattt tgaaccatta acagaagaag aagttttaaa tataatagaa
    30421 aaagaaaaac cagaaggtgt aatactacag tttggtggac aaacagctat aaaattggct
    30481 aagtttttac atgaaaaaaa tatacctata ctgggaacag actttagaga tatagatgca
    30541 gcagaagata gagaaaaatt tgatgattta ctagagagac tagatataaa tagaccaaaa
    30601 ggaaaaggtg tatggacaac taatgaaggt gttgaaattg ctaaggagtt aggttatcca
    30661 gtattagtta gaccatctta tgtacttggt ggtcaaggta tggaaataac ttataacgaa
    30721 gaaaaactaa ttcagtattt ggatgatgca tttgatagag accataaaaa tccagtactt
    30781 atagataaat atctaactgg tagagaaata gaagtagatg ctatatgtga taaagaagat
    30841 atattgatac ctggtataat ggagcattta gaaagagctg gagttcactc tggagatagt
    30901 acaacaatgt atccaagtca aaatatatct gatgaaataa aagaaaaaat agtagaatac
    30961 actaaaaaaa tagctttaga ccttaatgtg cttggtatgg ttaatataca atttatagaa
    31021 tttcaaaatg aattatatat aattgaggtt aaccctagag ctagtagaac agttccatat
    31081 ataagtaaag taagtggagt accaatagtt gacttggcta ctaagtgtat gttaggagct
    31141 aaattaaagg atttaggata tagtacagga gtttacaaag agccaaaatt agtatcagta
    31201 aaagtaccag tattctcaat gtctaaatta tctaaagttg aagtaagtct aggacctgaa
    31261 atgaagtcta caggagaagt tctaggtgta ggagaaaatt tagaagaagc cttatataaa
    31321 gggttcttgg cagcaggtag acatatgtct gatgaaagag gagtagtact tgctactgta
    31381 aataatcatg ataaagatga gtttatagag atagcaaaag atatgaaaga attaggatat
    31441 acttttgttg ctacagaagg aacagcgaag agtttaagag aaaatggaat agaggctgat
    31501 atagtaaata gaattgaaga gcctagacca aacatattag atgctataag aaataaacaa
    31561 gttgatatag taataaatac accaactaaa ggaaatgact ctacaagaga tggatttaag
    31621 atgagaagaa cagccattga attttctact gaaataatga catctttgga tacgctaaaa
    31681 gctttagtag aagttaagaa gaaacattta aacaaagata aattaaaagt ttataatata
    31741 gctgagtaaa agtgataagt ttgcaaagtg gtaaatttgt tctataagag gttataaatt
    31801 ttgtataaca aaagactgta gataaatttt atattctata cgagtttatt tatagccatt
    31861 tataaaaatt gtatagtatt taaatcattt taagataaag ggcattagct atagaagcta
    31921 ttgcctttta ttttaataaa tggataaatt taatttttca ttgatgtatg aatataagct
    31981 tgtaagatgt gtcatcaaga aagtagtgtt agtaggagtg tatgtttata agttagtgag
    32041 ttaaaaaact tgcacttaca ttatgttttt tgtcttatct tgtgtattta tgttataaga
    32101 aatataataa atcaatggag gtattatgca tggattatat aagaacaaaa gaaactactg
    32161 aaaattgtgt aatgataagt aaaatagtag tgtactattg ctctgctgga atatataaaa
    32221 ctaataaaat aaaggaggat gaaagtaaat gaaacggata tttttagttg aggatgataa
    32281 agcaattgcc aaaaatctta ttcttttgct taattcagaa ggatttatag taacccatgt
    32341 atccacaaga agtgaagcac ttgtagagat tgctaagaat aaatttgatt tggcacttat
    32401 tgatatttct ttacctgatg gaaatgggtt tacgatttgc acagaaatca aagaaaagca
    32461 agacattcct gttatctttt tgacagcttc cggtgatgaa tcaagtgttg ttactggact
    32521 gaatatgggt gcagatgatt atattactaa accttttcgt ccacgtgaac tgattgcgcg
    32581 aattagaaca gcactacgaa aaagtgggcg ttttgggtca acttttgaaa tttgtgggct
    32641 ttatgtagat acggcaaccg gtgttgtgaa aaagaatggc aatgaggttt ttctttcagc
    32701 tttagaatac cgtttgttat tggtgtttat tagcaatcct aaaagtatta ttacgagggt
    32761 aagattactt gatgaattgt gggatgcggc aggtgagttt gttaatgaca acacattgac
    32821 tgtgtacatc aaacgcttgc gagagaagat agaagatgac ccttcaagtc cacaaataat
    32881 tttgactgtt cgtgggacag ggtatagatt ggggggaagt tatgtttcga aatagagagt
    32941 ttcgaaaatt tgctatttta ttcttattaa ttactattct cactgtagcc ctgggatttg
    33001 caatcagtat tatgactgga atactttcta ttgtttctgc cactaccttt ggaatagcat
    33061 tttttgtgtt tactaaaaat cgatacaaaa gtattgcaca gatttcagaa caaattaatc
    33121 ttgtgcttca taatgctaac catctgtata ttgctgaatc agatgagggt gagctttcta
    33181 ttctacaaag tgaaataact aaaatgacgt tacgtattag agagcaaaat tacgcactga
    33241 aaaaagaaaa agaacacctt gctgactcac tagctgacat agcccaccag cttcgcactc
    33301 ctcttacatc agtaactctt attttgtcac tgttagagaa tacttctgat gaagatgaga
    33361 gaaaagagtt gatacgagaa acagaggaat tacttatacg aatggactgg ctgattactt
    33421 cccttttaaa attatcacgt ttagatgcag gtattgtagt ctttcaaaaa gaacaaattg
    33481 atgtgaataa tttgattagt tctgcacttc atcaactttt gattcccatg gaactgcaca
    33541 atatcacttt acatattgat ataccaaaag gagtaaggat tttgggtgat ttaaattggc
    33601 tttcagaagc aattcaaaat atatttaaaa actgcatgga gagtgtgggt gataatggaa
    33661 aaattgatat catttgtgag gacaactttt tgtttactca acttactatt catgacaacg
    33721 gagcaggctt taaaaaagaa gatttaccat gtctatttaa cagattttat cgtgggaaaa
    33781 attcaaacac agctggttat ggaataggat tggcactttg taagacgatt attatgagac
    33841 aaggaggaac aattactgca caaaatcacc cacaaggtgg tgcaatattt gttattcgtt
    33901 ttccaaagtg acgaatctct catttaaatg tcacttaaat gtaagttgaa agtcctatga
    33961 tatatttata aaatatgaaa ggagacttat ataatggcgt ttttaaagat tgaagattta
    34021 tgtaaggttt atggtaagaa tgaaaataag gttactgcac ttgaccatgt ctcacttact
    34081 attgaaaaag gagagtttac tgcaatcatt ggttcttctg gttctggtaa atctacacta
    34141 ctacattcca ttgcaggagt agacgtacca acaagtggaa agatatatct ggaggggcaa
    34201 gatgtatatg gccaaagcaa tgaaaaactt gctatttttc gtaggcgaca agttggattg
    34261 atttatcagt ttcacaatct gatacccaca ttaaatgtag tagaaaatat cacattgcct
    34321 atacttatgg ataagcgtaa ggtcaatcaa gaacggttaa atgacttgtt agaattgctt
    34381 ggtttaaaag aacgaaaaac acatttacca aatcaacttt ctggtggcca acagcaacgt
    34441 gttgccattg gtcgtgcatt gatgaatgca ccagcagtta tgcttgctga tgagcctaca
    34501 ggtagcttag acagtaaaaa tggacaagag attatccagt tactgaaaga gagccatagt
    34561 aaatatcacc aaactctcat tattgtcaca catgatgaaa acattgcttt acaggcagat
    34621 agaattatct gtatttcaga tggaaaagta gtgcgtgatg agaggaaggt gaatcgttaa
    34681 tgaacatttt taacaaagtt gctctgcaag gattgaaaaa gaatcgcact cgaactcttg
    34741 taacaattat aggagttgtt ctgtcagctg ctatgattac agcagttact acctttggag
    34801 tttctttgat gaactatatg acaaatggag ctattataaa atatggtgat tggcatgtcc
    34861 agtttttgga tgtagattct tcttttgtac aggaacaaag agtcaatgat gaagttgcaa
    34921 atactgcaac atttgaaaat attggctatg caacacttga aggtaacaga aatccagata
    34981 aaccatatct ttttattgct ggatttagca aagaagcttt tgatactttg cctataactt
    35041 taatttctgg aagattacct gaaaatagta gagagattct tgtttcaagt aaagttgcga
    35101 cagatggtgg tgtcaaattt gcagtaggtg atactatttc tttggatgta gggaaacgaa
    35161 tgagtgaaaa tgaaaagctt ggtcagagtg acccatacac tgctgggagt gaagtttttg
    35221 tgccacagga taagaaaact tatacagttg tgggtatctg tagaaagccc gtatttgaag
    35281 aatcttctgc accaggatat accttgataa ccaaagaaga taaacaagtc aagactgaca
    35341 gtcttagcct ttttgtcaca ttgaaaaatc cacatcagat tcgctcttac ataagtagca
    35401 ctaaagacca tgcttccatt ttcaatgaca atgtgttgcg cttcataggt gtttctgata
    35461 acaatttgtt caatatgttt ttgtactcgg ttggtggcat tgtgcttgcc ataatcatgc
    35521 taggctcaat ttttctaata tataattcat ttaacatgtc attgaacgag cgaacacatc
    35581 aatttggaat actttcatct gttggagcta cagcaaaaca gttacgaaat tcggtgctat
    35641 ttgaaggaat ttgcattggt gcgattggta taccaattgg tgttgcaatc ggtatagtca
    35701 gtattggact tgtgatttct gtcatagcta aaaactttgc aaatattctc tatgtaaacg
    35761 ttccgttgac attaacattg tctatccctg caatcatcgt tgcagcagtg gtaagtatgg
    35821 ttacaatctt aatttctgct tacataccag caagaaaggc tgccaacaca ccagtgatgg
    35881 agtgtattcg tcagacaaat gaggtaaaaa ttgaatctaa atctgtgaag acatcaaaac
    35941 taacacagcg agtttatggt cttgagggag ttcttgctat gaaaaacttt aaaagaaaca
    36001 agaaacgtta tcgaagtatt gtattgtcgc ttgttttaag catagtttta tttatatcta
    36061 caagtgcttt agtagcagac ctaaaacaga tgtcaaatca gaggtcagag gttactgacc
    36121 aggatattat tttttctaca caaggtatga aagacaacga aatgttgaag ctttacgata
    36181 agctaaaaac tgcaaatggt gtttacgaag gtttgtatca gaaacttatg acctattcat
    36241 gtgttgccaa agtaagtgag cttacagacg attatttgaa ctatgagggt tcacattcta
    36301 tgagtgaaat gcttgaatta ccagtagata tgcaatttct tgatgataga acttatctga
    36361 atattattaa aagtcttgga ttaccagcag aagaatatac tggacaaaat gcaaaactga
    36421 ttgctgtttc tatggtaaca aaggataata atattattga tattttcaac agttcttcta
    36481 tgaatgttag cctcactcct gaaactaatg gcaagttgga gatacctgag agacaaaatg
    36541 tgaatatttc atttgtcaaa gtagtgccac ctgattctcc tccaggaatc agaagttcag
    36601 atgtgaagaa agcaagcttc agagtattgg ctccttattc agccaaagat aagtttgaga
    36661 ttcctggtgt ttctacagga attgaaagtt taagctttca atcgaaaaat cctactcagt
    36721 cagttgcaga aatggagcaa attattgaga gtgagggagt tacgaatgag tacaatctgt
    36781 acaatatgta tggaatgatg gatgagaacc gtaattacat ttttattgcc aatgtttttg
    36841 cttatacttt cataattatg atttctctga tttcgattgc caatgtgttt aatacaattt
    36901 caactaatat taagttacgt cgtagagaac ttgccatgct tcgctctgta ggaatgtctg
    36961 agcatgattt ccaaaagatg atgaattttg agtgtgcctt ttatggcatg agagcattgt
    37021 tctttggact tcctatagca gtaatttctt catggctaat tcataaagta atggttacag
    37081 atgatattga ttttgtgatt ccatggttta gtattggaat tagtatattt agtgttttat
    37141 ttatagtatt tattacaatg ttgtatacta ttaataagat aaaaaaagaa aatattattg
    37201 atgcacttcg tgatgacatg gcttaattaa aatgatatgg attaattaaa aataggaggg
    37261 aattagcatt agccatattt cctcctattt tttgaaaatc tcactgttag gaatcactat
    37321 ctagaaaaat catatgttga taatagacaa ctatattaag atgtgtaagt aaactagatt
    37381 actacactag acatcttaag tcaatttctc tctttttaac ttgtatttga atttctgtta
    37441 gaagttcctc ttcgttgttt gtagacagag ggtctattat aggtatttca tatatataat
    37501 tttcaatagg tattctttta tattcaacat aatcaaccag tttcttaaaa gcggtataat
    37561 tttctttgta aggcccttta tatgtcatac aaataaaatc accagatgaa atttctgtta
    37621 tatgtttttg tgattttata tcaaataagt ctctttcaac agtaacaaaa ctattgtcat
    37681 aaataacttt atcttctttt aaaaatttat ctatagatac aagtccactt actccaccat
    37741 agaacacaga tatattatct tctaatatat ttgctatttg tcttctggaa agttccatag
    37801 tttcttcact acttaatcct tcttcatact ctatagataa gatacttctt cgtggaatat
    37861 gttttctcac tattttacca acttcatcta ctttcatacc tttttccata gtttctttct
    37921 ttgtgcttag taccttcttt ataagctcta attctttaat tttttcatcc actatattaa
    37981 ttttatttgt tagtaatttt agaatttctt cattattttc atcaagcttt gacttaattt
    38041 cttttaaagg catacctaaa tattttaaat attttataat atctaattgt gaaaattggt
    38101 caattgagta atatctgtaa ttgctttctt ggtcaacatg aacaggttta aataaatcta
    38161 tttggtcata gtaccttagt gtctgtatgc ttattttatg aagtttagac atttctccta
    38221 ttgaaaattt tttactcata taatcacctc atttgagatt taaaatatat tccatggata
    38281 tttgtcaggt ggatgacatg taaattccat tttttctttt gtagtaggat ggcaaatttc
    38341 tatcttattg gaccaaagag atatttgttg tccaatctta tttatttctt tgccatatct
    38401 ttggtctccg tataatggat gttttctgct tgcaaattga actcttattt gatgagaacg
    38461 accagtcttt aaattaactt gaactaaact tagattttct ttaaaatcta atacattata
    38521 gttaagttca gcttctttgg catctttatg gctttttttc actacagtta ccatattagt
    38581 ttttttattt ttatataaaa aatcttttag tatatctgag ttagatttta tcttgccatg
    38641 aacaacagct agataagttt ttttcaaagt cttacttctt acctgctcag acaatctaga
    38701 tgctgctttt gatgtttttg caaacaccat aactccacct actggtctat ccaatctatg
    38761 tattaaacca atatatacat ttccaggttt gttatacttt tttttaatat agtcttttaa
    38821 aagatttacc atatctttat cattagtatt atctccttga gacaatatgt taactggttt
    38881 ttctacaact aataaatggt tatcttcata tatgactttt atcattattt tttctcccat
    38941 cttccaaata taccacaagg aagtatctta ccatctctgg aagctggtat tcctaattcc
    39001 ccaccgtata tatctcctgt atttgtcttt ttacctattg ttgtagacaa tacattttca
    39061 agaactattg gagaaaatcc tgttgtataa gaattaatta agaaaaataa agggttatct
    39121 gataaaactc ccatacataa atctacaaaa ctgtataact tttcttctat ttgccatact
    39181 tctcctttag gacctcttcc ataagaagga gggtccatta ttattgcatc atatttttta
    39241 cctcttctta tttctctttc aacaaacttt acaacatcat ccactataaa tcttactgtt
    39301 tgatttttaa gacctgataa ttctatattt tcttttgccc aattaaccat accttttgca
    39361 gcatcaacat gacatacttc tgcaccagca gatgcacatg ctacagtagc tccacctgta
    39421 tatgcaaaaa gattcaacac ttttataggt ctgttagctt tctttatttt ctctatcatc
    39481 caatcccagt tagctgcttg ctctgggaat aatccagtat gtttaaagcc agttggttta
    39541 atgtgaaact ttaagttttt ataatttata gtccattgtt ctggatatct ttttttgtgt
    39601 tcccattgac ctccaccttt attgcttctg tgataatgtc catgagggtt tttccataaa
    39661 gcccattcag attccattgg ccatacaacc tgtgggtctg gtcttctaag tacataactt
    39721 ccccaacgtt ctaatttttc tccatttccc atatctataa gttcataatc tttccattta
    39781 tctgctaata ataacatatt tttcctccta taaaataact ctcaatccta tgtattatac
    39841 cacaaaatta gtcatttttt gctgttttag atactttcta ttctttacat ttaccattaa
    39901 cattattact gttttaagca tattatattg tatctgattt ttttataaaa gagggaggat
    39961 tttaatggaa aattatcaat ctacaaatac ctgtacatat aatatattat atgtacaatt
    40021 taatgaagat tttgaaagaa gtaagtattt ttcatatgat gtaaagtatc ctgttatata
    40081 caatattaga aatcaatcca cgtatgtaaa cccagttatt ttaaataaca taaacaatgc
    40141 tgtaagaatg actgtaaata attttaaaaa tggcttacag gaagaagaac gagaatataa
    40201 taacgcagct tctcaaaatt cacttccaaa acaaagctat tgggtatcta ccagttacgc
    40261 agttactttt aataaaaatt atgtactgag tatgatatta aaccttatgg ggtttgctaa
    40321 taactatggg cctaaataca atacattgaa taattacaat atagatttga caactggaaa
    40381 agaactttta cttaatgaca tttttagaga tggagttgat tatattaata taattactga
    40441 ttacattaaa tctcaaataa atgaaaatag agaaatgtat tattctaatg ttgagttagt
    40501 tatacctgaa gaccaagcat tttatcttac agataatgga attatcatct attttggcgt
    40561 tgacgaaata gcacctgaaa gttttggtat acctaagttt gaactgaagt tttctgactt
    40621 tggaaatttt ataaatttac gcttctattg tatttcccca gatatatatg ttcaatcaag
    40681 aggaagagct aagcatttta tgggataaaa atatagataa ttctaagccc tattttagta
    40741 tttatgctgg agtagggtta agatttatct atcttaaata atgtgattta aacaatacta
    40801 ttcgtatgtg ttaattatat aaatttagac atattgataa atgttaaaat agaatgattg
    40861 ttttgt
//
LOCUS       DAGSWI010000022        40676 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.022, whole genome shotgun sequence.
ACCESSION   DAGSWI010000022 DAGSWI010000000
VERSION     DAGSWI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40676
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.022"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            123..239
                     /gene="rrf"
                     /locus_tag="MJF80_001036"
     rRNA            123..239
                     /gene="rrf"
                     /locus_tag="MJF80_001036"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            359..550
                     /locus_tag="MJF80_001037"
     CDS             359..550
                     /locus_tag="MJF80_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBZ0279709.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            705..2114
                     /locus_tag="MJF80_001038"
     CDS             705..2114
                     /locus_tag="MJF80_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBZ0279710.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNILKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            2245..2934
                     /locus_tag="MJF80_001039"
     CDS             2245..2934
                     /locus_tag="MJF80_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBZ0279711.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2937..3872
                     /locus_tag="MJF80_001040"
     CDS             2937..3872
                     /locus_tag="MJF80_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBZ0279712.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3869..4762
                     /locus_tag="MJF80_001041"
     CDS             3869..4762
                     /locus_tag="MJF80_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBZ0279713.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4840..5586
                     /locus_tag="MJF80_001042"
     CDS             4840..5586
                     /locus_tag="MJF80_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBZ0279714.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLIQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5587..6420
                     /gene="rsmI"
                     /locus_tag="MJF80_001043"
     CDS             5587..6420
                     /gene="rsmI"
                     /locus_tag="MJF80_001043"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBZ0279715.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6443..7147
                     /locus_tag="MJF80_001044"
     CDS             6443..7147
                     /locus_tag="MJF80_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBZ0279716.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDRDFFPIHLRKHVSNYLKTYIEEGINKLNVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7335..7763)
                     /locus_tag="MJF80_001045"
     CDS             complement(7335..7763)
                     /locus_tag="MJF80_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBZ0279717.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7800..9230)
                     /locus_tag="MJF80_001046"
     CDS             complement(7800..9230)
                     /locus_tag="MJF80_001046"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBZ0279718.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9362..9946
                     /locus_tag="MJF80_001047"
     CDS             9362..9946
                     /locus_tag="MJF80_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBZ0279719.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMKRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9962..10483
                     /locus_tag="MJF80_001048"
     CDS             9962..10483
                     /locus_tag="MJF80_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HBZ0279720.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10549..10795
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10879..12816
                     /gene="metG"
                     /locus_tag="MJF80_001049"
     CDS             10879..12816
                     /gene="metG"
                     /locus_tag="MJF80_001049"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBZ0279721.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKGELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12846..13616
                     /locus_tag="MJF80_001050"
     CDS             12846..13616
                     /locus_tag="MJF80_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBZ0279722.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13954..14385
                     /gene="phnG"
                     /locus_tag="MJF80_001051"
     CDS             13954..14385
                     /gene="phnG"
                     /locus_tag="MJF80_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBZ0279723.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14400..14975
                     /gene="phnH"
                     /locus_tag="MJF80_001052"
     CDS             14400..14975
                     /gene="phnH"
                     /locus_tag="MJF80_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBZ0279724.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSTINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14978..16063
                     /locus_tag="MJF80_001053"
     CDS             14978..16063
                     /locus_tag="MJF80_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HBZ0279725.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRDFRDSFISELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            16083..16952
                     /locus_tag="MJF80_001054"
     CDS             16083..16952
                     /locus_tag="MJF80_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBZ0279726.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16945..17811
                     /locus_tag="MJF80_001055"
     CDS             16945..17811
                     /locus_tag="MJF80_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0279727.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17852..18565
                     /locus_tag="MJF80_001056"
     CDS             17852..18565
                     /locus_tag="MJF80_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0279728.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18566..19735
                     /locus_tag="MJF80_001057"
     CDS             18566..19735
                     /locus_tag="MJF80_001057"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBZ0279729.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19739..20575
                     /locus_tag="MJF80_001058"
     CDS             19739..20575
                     /locus_tag="MJF80_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBZ0279730.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20576..21571
                     /locus_tag="MJF80_001059"
     CDS             20576..21571
                     /locus_tag="MJF80_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0279731.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21589..23277
                     /locus_tag="MJF80_001060"
     CDS             21589..23277
                     /locus_tag="MJF80_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBZ0279732.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23289..24356
                     /locus_tag="MJF80_001061"
     CDS             23289..24356
                     /locus_tag="MJF80_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBZ0279733.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24793..25788
                     /locus_tag="MJF80_001062"
     CDS             24793..25788
                     /locus_tag="MJF80_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0279734.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIDKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHRKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
     gene            25806..27494
                     /locus_tag="MJF80_001063"
     CDS             25806..27494
                     /locus_tag="MJF80_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBZ0279735.1"
                     /translation="MITIKNKRRISDSKIQKCFLVFLVIALVVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTNIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMSITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            27506..28573
                     /locus_tag="MJF80_001064"
     CDS             27506..28573
                     /locus_tag="MJF80_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBZ0279736.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            28765..29295
                     /gene="rnmV"
                     /locus_tag="MJF80_001065"
     CDS             28765..29295
                     /gene="rnmV"
                     /locus_tag="MJF80_001065"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBZ0279737.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            29466..30335
                     /gene="rsmA"
                     /locus_tag="MJF80_001066"
     CDS             29466..30335
                     /gene="rsmA"
                     /locus_tag="MJF80_001066"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBZ0279738.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEIGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            30401..31663
                     /locus_tag="MJF80_001067"
     CDS             30401..31663
                     /locus_tag="MJF80_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279739.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDSEEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            31814..32929
                     /locus_tag="MJF80_001068"
     CDS             31814..32929
                     /locus_tag="MJF80_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBZ0279740.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKVLGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            33115..34011
                     /locus_tag="MJF80_001069"
     CDS             33115..34011
                     /locus_tag="MJF80_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBZ0279741.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            34081..34878
                     /locus_tag="MJF80_001070"
     CDS             34081..34878
                     /locus_tag="MJF80_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279742.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(34933..36285)
                     /locus_tag="MJF80_001071"
     CDS             complement(34933..36285)
                     /locus_tag="MJF80_001071"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBZ0279743.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            36504..37382
                     /gene="purR"
                     /locus_tag="MJF80_001072"
     CDS             36504..37382
                     /gene="purR"
                     /locus_tag="MJF80_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBZ0279744.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            37538..37816
                     /gene="spoVG"
                     /locus_tag="MJF80_001073"
     CDS             37538..37816
                     /gene="spoVG"
                     /locus_tag="MJF80_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBZ0279745.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            38029..39408
                     /gene="glmU"
                     /locus_tag="MJF80_001074"
     CDS             38029..39408
                     /gene="glmU"
                     /locus_tag="MJF80_001074"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBZ0279746.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            39503..40453
                     /locus_tag="MJF80_001075"
     CDS             39503..40453
                     /locus_tag="MJF80_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBZ0279747.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
BASE COUNT        16207 a         4497 c         7313 g        12659 t
ORIGIN      
        1 atttgatgtt cattcattaa gatatatgtg tagtttttag agtgctaact ctaaagaaac
       61 tagtattact agattctata aacttattaa aaacttaata gaaatattaa gcataaagat
      121 tatgtggtta caatagcaga gaggatacac ctgttcccat tccgaacaca gaagttaagc
      181 tctctagcgc tgatggtact tggtgggaaa ctgcctggga gagtaggacg tagccacgta
      241 atttttttta ttttttgtca aaaaaaacaa aaaatacgta tttctttttt catttcggga
      301 aaactaaaca caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt
      361 gaaaaaagtt gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat
      421 tctaggtaca gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat
      481 aatatacttc attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga
      541 tagagaataa tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat
      601 ttaattttat aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa
      661 gttttaaaaa gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg
      721 aattaaataa gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg
      781 gacaggttta taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata
      841 caactgagat attaggaaca gataatttac actttccgga aggaattata aaaaattcac
      901 aaaaaaaagc atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta
      961 gctgtggaat acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa
     1021 atagagactg tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt
     1081 atgtgccttg tgagataagt gaagagacaa atatcttaaa agggatatct ataagtaatg
     1141 tgattgatat aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat
     1201 attatggaat gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg
     1261 tagtgatagt agatgaagca catggtgcgc atttagggct tagcgagagg cttcctaaaa
     1321 cagctcttga gcaaggcgca gatatagttg tacagagtac acataaaact cttccagcat
     1381 ttacacaatc gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga
     1441 acatgttaag aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat
     1501 tagcagtaga tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata
     1561 ttataacttt caaaaataaa gttgattcaa atattaatat atacgacaca aatgataaaa
     1621 caaaaatatt tatatcatct aaaaacatgg gtttaacagg ttatgaacta gaaaatattt
     1681 tgagaaataa atataacata caagtggagt tatctaatta ttatggagtt cttttaatat
     1741 gtacaatagg aaatactgtt gaggatttct ctagtttaga aacagcatta aacaatattg
     1801 taaaggaaga ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa
     1861 agaaaatatt aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg
     1921 acagcatagg taaagtttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa
     1981 tatctcctgg tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta
     2041 aaggcatgat tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa ataatagatt
     2101 tagaagacat ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg
     2161 aagagaaata aatagactgc ttatatagag ctatctaata aaagtactaa aaaagtaaaa
     2221 agtgtttgta tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca
     2281 gatgcaagtg gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc
     2341 tatatgatta aaaaggtaga atatccaaat tatgaatctc catcatctgc cttggtgaaa
     2401 atgtacctaa aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca
     2461 acttttttta cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat
     2521 gaaggtggaa taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct
     2581 aaaatggata tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat
     2641 ttatttaaga taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt
     2701 aaaaagctta tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat
     2761 gaaagtgaca tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa
     2821 tatgattgga aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata
     2881 cataatgaaa tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt
     2941 attttgaaaa tattataggg caagattttg caaagaaata tttaacaaat tctataaaga
     3001 aaaataaact taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat
     3061 ttgcagatga attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact
     3121 atgtactcat aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat
     3181 cagatatagt aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa
     3241 aaatgactgt ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg
     3301 ctataataat cttagtcaca aacaacaagg aatctttatt agaaactata aaatctagat
     3361 gtgatataat aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata
     3421 caggtataga agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga
     3481 atgctctaaa tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt
     3541 atattcagat aatgttagat aaaaacatag tggagatttt aaatatacca aataatatgg
     3601 aaaaatatag aggtaagatt atagctttat tagatatgat gataaactat tttcgagata
     3661 tcatcctatt aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt
     3721 tcattcaaaa tatgagtgga aaaattagtt attctcaatt gtccaagatt attgatataa
     3781 tagaagatgc aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta
     3841 tgtctttaaa tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag
     3901 tgctgggaaa atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt
     3961 agttgtagaa actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat
     4021 ggatgaaagc gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga
     4081 taaaaaagta tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca
     4141 aaaaataaaa gaacatgagt taacaatgtt tttaatagat tgtgagtata catttgatag
     4201 gaataagttg attttttact ttacagcaga aggaagaata gattttagag aattggtaaa
     4261 agacttagct gctatattta aaactagaat agaacttaga caaataggtg tcagagatga
     4321 agctaaatct ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt
     4381 aggtgatttt caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc
     4441 aacaaaaata tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt
     4501 atatagtgaa gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg
     4561 aaaaggaaaa gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga
     4621 caagacagta aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg
     4681 tgatggctgt ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga
     4741 gctaaagaaa ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat
     4801 taatgcttaa agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa
     4861 agaatagatg atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt
     4921 tttggtatag atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta
     4981 gtagatttgg gtactggaac tggcattata cctattttga tagctggaaa aagtgaagct
     5041 aaaaaaatta ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata
     5101 aaattaaatg gattagaaga aagagttgag attataaatg aagatataaa atacatagac
     5161 aaagtcttag acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt
     5221 gatggaataa aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat
     5281 ttggaagatg taattagagc tgcatccaga ctggttatgc ctagaggaaa attcttcatg
     5341 atacatagac caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa
     5401 ccaaaggtta ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttgatt
     5461 caatttgtaa aagatggaag gccagagctt aaaatattgg acccattgta tgtttatgga
     5521 gaagatggga attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag
     5581 ttgtaaatga gcgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata
     5641 acatatagaa ctttaagaat attaaaagaa gtagacttga ttgcagcaga agatacaaga
     5701 catagtataa aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa
     5761 cataataagg attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata
     5821 gctctgataa gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa
     5881 caagctatac aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg
     5941 gctttagttg gctctggtat ggacacacat agatttgttt ttgagggctt tttagataga
     6001 gataaaaaag ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt
     6061 ttctatgaat caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt
     6121 aataggaata tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa
     6181 aatatagaaa ctaccataaa aatatttgag gaaaaagaag taagaggaga gtttgtatta
     6241 atagtagagg gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat
     6301 agggaatatg ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata
     6361 gtttgtaaag atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa
     6421 aacctgaatc tgaggtgatt ttatggaaat aggaaaaatt ataatagaga gtttaaagag
     6481 ggaagataca cataaaatat ttatagaatt agataataag catgaattag aaaaaataat
     6541 tcctaaaata agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt
     6601 tgaacattct ataaatgcat taaaagaatt agaaatagta ttaaatgata gggatttttt
     6661 tccaatacat ctaagaaaac atgtgtcaaa ctatttaaaa acatatattg aagaaggaat
     6721 aaataagtta aatgtattaa agctgggaac atttttacat gatataggta agcctgacag
     6781 tatgacctta gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca
     6841 aataattgac aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag
     6901 taaatatgta agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag
     6961 gaaagaatta attaatgtat ttaacttagt agatgaggat actataggtg taataatgct
     7021 tgggtatgca gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt
     7081 gttaaagaca tatatggaat atattttaac aaattacttg tataagagta attatacaca
     7141 tgtataaatt tagttaatat aattgaattt tagatgatta attgaagttt gaaatatacg
     7201 gatttcaaaa acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt
     7261 atatcaaaaa ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta
     7321 gtatttaatt tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag
     7381 gtcttagtag aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct
     7441 tttacaatta cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc
     7501 ctagggtcct ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata
     7561 tgaataaatg cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct
     7621 tctataacac cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa
     7681 ccaactattt ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg
     7741 attctgtaat ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatgc
     7801 tataaaactt ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa
     7861 tcaaccttat aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat
     7921 tcatattgta catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct
     7981 tttatataca atggtttaag aagctcttta actttgtaat ttgtaaatac cttcgttttc
     8041 catgtataag taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt
     8101 aacataatca aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg
     8161 taaccaggat tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact
     8221 cctttttcac aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat
     8281 gtaatgagct ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat
     8341 gttatggtat attcgtctag gtcattagat gcagtgatgc tgatatctga aaagccagca
     8401 ttatctaatt gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt
     8461 atacccatag gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga
     8521 actccactgt ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca
     8581 tcagcatatg cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct
     8641 tgtgtaccaa ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca
     8701 caaccaccaa ctacggcagc cctagcccca tatatccctg catctggacc ttgtgcacga
     8761 cgtaatccaa attcaaatac agggtctcct tgagctgcaa agcaaactct agatgcttta
     8821 gtagcaataa gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct
     8881 tgatataaag gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc
     8941 tcaacagcat atatatctcc tgtgaattta aattctttta aaaattttaa gaacttgtca
     9001 gaaaataaat ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct
     9061 atgtattcaa caacctgatc aataccacaa actatagtat accctccatc acaagcgttt
     9121 tttctaaaaa acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca
     9181 tttaacatag taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc
     9241 cttaattatt attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat
     9301 atagtacaac tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag
     9361 gatgattata ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt
     9421 gatttctaaa aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat
     9481 agagtttgca ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc
     9541 aaaagaatca ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgaaaagatt
     9601 atttccttct attccaaaag gtcataaagc aatgtcttat atagttatga atattgcttt
     9661 aaatatgttg ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct
     9721 tcaaactttg aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat
     9781 caatatatca tctatacaaa taattccatt tactataatc aaattaagga tggatatggg
     9841 ggctcaaaat ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc
     9901 agtagcaata ataacatgta aattttttca aagaagatat agataaaggg gatgtagata
     9961 catggagata ttttcaaact tacttatacc aataataatt ctgtacatag taatttatgg
    10021 taaatataaa aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atggattaaa
    10081 agctgcatgg gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa
    10141 aactggtaaa ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag
    10201 tataccaaaa gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt
    10261 agggatgtac agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg
    10321 tgctacgatt gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag
    10381 tctgaagata aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg
    10441 tgtcatagca gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg
    10501 aaaaatttta taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg
    10561 aaaagaagag taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt
    10621 caaaagcttt attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa
    10681 acggttggac acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga
    10741 aacataacta ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac
    10801 tcataggagg cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa
    10861 aattaggagg taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat
    10921 ccaagtggag gattacacat aggacatact tattcaacag tagctgcaga tacaatagct
    10981 agattcaagc gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt
    11041 gaaaaaattc aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga
    11101 atgataaaag atataaaggc cttatggaat actatggata tatcttatga tgattttata
    11161 agaactacag aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag
    11221 caaggtgata tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc
    11281 tggacagaat ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat
    11341 ttagtaaaag aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag
    11401 ttatttaaag atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac
    11461 tttttagaca aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata
    11521 aaagttcctt ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat
    11581 ataacagcat taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc
    11641 aatgttcaaa tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt
    11701 ttaatggctt taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt
    11761 gctgatgata aaatgagtaa atcaaagggt aatgtggttt atccagaacc tataatagaa
    11821 agatatggaa tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat
    11881 ggaagttata ctcatagaaa ttttgtaaca agaataaatt atgatttagc aaatgattta
    11941 ggaaacttaa taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca
    12001 acagcaaaag tttcaactga ttttgatgct gacttaaaag aacaagctgt ttcaactaga
    12061 gaaaactttg aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg
    12121 aaattagtta gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa
    12181 gatgaaacga agaaaggtga attagacaca gttttatata atttatgtga atctataaga
    12241 ataatcgcaa cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat
    12301 ataggaataa aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt
    12361 attggagaaa atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa
    12421 aaagagatag aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct
    12481 ttagagcata aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg
    12541 aaaataataa gctgtgaaaa acatccaaaa gcaaataaat tattagtatc acaagttaag
    12601 attggaccag aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat
    12661 ttagtaggaa aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta
    12721 gaatctcaag gtatgatatt agcagcaggc gatgatggag acccatatgt tttaccattt
    12781 acacaagggg ctaaagatgg atgcgaggtt cgttagtagg taattaaata gtaaggagat
    12841 taaagatgtt atttgactca catgcacatt taaatgatga aagttttgat gaagatagag
    12901 atgaacttat tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag
    12961 atatagagac gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag
    13021 cagttggggt acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa
    13081 gaaaacttgc aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt
    13141 attatgatta ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag
    13201 ctaatgagct aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg
    13261 agataataaa gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggga
    13321 atgtagagtt agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggcacgg
    13381 ttacttttaa gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat
    13441 tattcataga gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca
    13501 atccttccca agtatctttt gttgctgata agatagccca ggaaaaaggg atatcttatg
    13561 aagaagtatg tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa
    13621 attaaatctt ataatgatat atataataag tcattataag tataggatga gagtgcctca
    13681 aaatgagttt tttagttcat gaggcactct tttttttatg aaatcaaata tattttcatc
    13741 atttcaacaa tgaaaaagaa gtttatcata ttttgagata gtttgttttt atttatttaa
    13801 ttcatatttt aaaatatatt ttcttaacat tgttaaaaaa gaatttacaa gatattcatt
    13861 agaattaact caactctaat attcacttaa cttaagttgt ttattatatt attaaagaga
    13921 agtaaacgaa aggtagatta aggaggttga attttgtata cagttgatag tactaagata
    13981 ttgataaata tgaacttaga gaaactagaa aagatatact caatgataaa agatacatca
    14041 agtatttcta taattaaaga acctaacttg gctacagtaa tggtaagagc taatgaaagt
    14101 gtaaaaaaca caacatttaa tttaggagag atattagtta ctgaatgtag tgtaaaggta
    14161 gatgaaagct tgggttacgg aatagtttca gaaaacaata ataagaaagc catttattta
    14221 gcagttatag atgcagtttt acatagcaat aacagtaaat ttgatgagtt aaagaattac
    14281 ataaataaaa gtgtatatga agagaactta aggtatgaac aagagattat tgatgaattt
    14341 agcttgatta ataaaacgaa agttcaattc aatgctatgg attaaatagg aggagaatca
    14401 tggtaaatta tacacaagaa atatatagaa agatgctaga tagtgcttca tttcctggga
    14461 aattaaataa tatagataat ataaatatag aaaataatac aaaactattg aatggagcaa
    14521 ttggtatagc aatcacttta ttagaccaag aagttacctt ctatatagaa aattatactc
    14581 aaaaagatat aaaaaatata agagcgctta caatgtcaaa tcaaatagat tatagaaaat
    14641 cagattatat atttttagat attaattcag aagtggatat attacagatt aaaataggtt
    14701 cttttgaata tcctgatgaa agtgcaacta tagtacatca agtaaatgat ttatcaacgt
    14761 actctcaaac taagtatata aagcttgatt tgagtggtcc tggtataaaa tctaaaaata
    14821 gtatatatat aaatggaatt agagaagaat ttatagaaag attatctacc ataaataaag
    14881 attatccaat aggaatagat ttgattttag tagataaaaa aggagaaatt gcatttatac
    14941 caaggtcatc aaaattaagt tgggaggtat tgtagaaatg gcttatgtag cagttaaagg
    15001 tggagaagaa gccatattac aggctaaaaa tatacttgag ttttataggg taaaaggaga
    15061 tagcttgcct atagatgtaa agcagattga ggaacaaatg tcttatgctg tagacaaagt
    15121 aatgtctgaa ggaagtttat acaacaagga actatcagca ttggctataa aacaagctca
    15181 aggtgatata ttagaagcct ctttctatgt aagagcttta aggtcaacaa tgcctagaat
    15241 tacatattca gaacccataa atacagaaga aatgagaata aaaagaagaa tatcagctac
    15301 ttttaaagat ataccaggag gtcagtatct tggggcaact agagactatg aaaatagaat
    15361 attgaatgta gaattattag aagaagatga aaaaaagaat agagatttta gagatagttt
    15421 tataagtgaa ttagaaacag tagatgaaat ccctgaattt acaaaaatat ctaatatatt
    15481 tagaaaagaa aatttattac aagaagaagt ttgtgaaaac aaattggaag aagttattga
    15541 tgatattaca agaaatagtt taagatttcc agtttctagg tcagcaaagt tacaggcatt
    15601 aactagaggt gaaagtggag tagtgtcagg ttttgcttat gctattgtaa gaggttttgg
    15661 tgatgaacat ccatatataa gtgaacttag aactggtaat gtatttataa aagtaaaaca
    15721 tcccatctat gaagatgaag aaataataat aggtgatatg ctggttactg aatgtgaaat
    15781 agtttcaaaa gcatcacaaa ataagactga aaattctaaa actaattcgg aagataaaaa
    15841 taagatgtct ttaggatatg gtctttgtat ggggaataat gagaataagg ctatatctat
    15901 ggccatacta gacaaatctt tggagccaaa tagtgaaaaa ctagcacaag atgaagaatt
    15961 tgtattgctt catatagatg gaatagattc attaggattt atatctcact ttaagcttcc
    16021 tcactatgta gattttaaag cggatgtaga aagaataaaa tagtttgtgg aggtaagttt
    16081 aggtgatata taactatggt tttttagacg aaaacacaaa aaaagaaata agaagaaaga
    16141 tattaaaagc aatatcaata cctggttatc aagttccctt tggttcaaga gagttgccta
    16201 tagctaaggg atggggtact ggaggtttac aattaacact atcattaatt ggtaaaaatg
    16261 atgtagtaaa agtaattgac caaggaagtg atgattctac aaatgcttgt aatataagga
    16321 gctttattgc aagtgtaagt aatgtagaaa ccacaaaaga tacattagaa gctacattga
    16381 ttcaaacgag acatagaata ccagaagaaa aacttaaatc taatcaaata ctaatatttc
    16441 aagtgccaat accagaaacc cttagaatag tagaacctag tgaagtagaa acaagaagaa
    16501 tgcattcaga agaagattat agtaggatgt gggtatatct ttatgaagat atagttagat
    16561 ttaatgatat atctatagca gttgaatatc catgtaaagt aaatgataga tatttaatga
    16621 atccatcacc tatacctaga tttgatataa aaaaattaaa tatgtcagac aatttattct
    16681 tgtttggtgc aggtagagaa aaaagaattt atgctattcc tccatataca aaggtagaac
    16741 ctttagagtt tgaggattat aagtttgaag aggaaaagtt tgaaggaaaa tactgtagtt
    16801 tatgtaacag tacaaataca tttttagatg aagtatatga cagtgacact aatgaaaagt
    16861 actatagttg ctcagataca agttattgtg aaaaagttag attaaaaaat aacagtatag
    16921 atgtgacaat aggaggtact tggaatgagt aaaataaatg actctaaaag attagaaaat
    16981 atagacaagc aagtattaat aggaagaaat atcaatctta tttatggaga tgggtgtgaa
    17041 aagtgttttg aaagcacagg atatgaaagc aataatatat gtaagtattg tggttctata
    17101 gttgcatgta ataatgtaaa tttagaactt tatgaaggag aagtccttgg aattgtaggt
    17161 gaatctggtt caggaaaaag cactttatta aaaatactgt ttttccaaga acatgcaaat
    17221 agtggagaag tatatatatc ccattacaat aaagacacaa atatactaga gctttcagac
    17281 caaaaaaaga gatacataaa gaatcatttt atgggtattg tgtatcaaaa tccccacctt
    17341 ggtttaaatt tagactttag tagtggtggt aatatagcag aaaaattatt gatggcaaat
    17401 ttatataatg ttgaaaaaat aagagataga ggcaaagaac ttttaataaa aactaatata
    17461 cctgtagaaa gaatagacca taaaccaaaa tactttagtg ggggaatgca acaacgagtt
    17521 caaatagcaa aagctctttc aaataaccct ccaatattgc tattagatga ggttacaaca
    17581 ggtttagatg tttctgtgca agcggaagta ttggatttaa taagagaaat ccaaagagaa
    17641 cttaagattt ctatgatagt tgtatcccat gactttgatg ttataaagat gcttgctgat
    17701 aggacaattg taatgaaaaa tggaagtgta attgagtctg gtcttacaga ccaaataatg
    17761 gaggacccac aacacccata tactcagcaa cttataaatt cattgctata aatatcgttt
    17821 gtaaataagc aaaatggggg tttttaataa aatgaaagaa actatacttg aagtaaataa
    17881 ttttagtaaa gactttgagt tacatgcatt gaacaagact ataacggcat gtagtaatat
    17941 aagttttgaa gtttcaaaag gtgagttttt aggaataata ggtaaatctg gagcaggtaa
    18001 gtctaccata ttaaaaagta tctataaaac atatatacca acaactggag aaataatttt
    18061 taattcagaa atatatggta atgtagacct ggctaaaatt ggagaccgag aaatgataaa
    18121 tcttagaaag aaagaaatag gatatgtatc acagttttta aagacacttc cacgaataac
    18181 tgcaattgaa ctggtagtac attctttaat tgagagtggg tttgaaaaag attgttcgta
    18241 tgatatggca aaagatatac ttaaccaatt tgaaataaag gaaaatttat gggatgcgta
    18301 tccaaataat ttttctggtg gagaaaaatt aagattaaat ttagctcaag ctatggttaa
    18361 aaagcctaga cttttgttac ttgatgaacc aactgcatca cttgataatc aatctaaaat
    18421 atatgtaaaa gaaaaaatgt tggagcttaa aagtcaagga actacaatga taggaatttt
    18481 tcatgatata gagtttatgg aaactgttat agataagacc tttactatga caaaagggac
    18541 tataagtgaa agtggggtag cctagatgat aaaactaatt cataatgcaa atattgtatt
    18601 agaagatatg attttagaaa atggtcattt aatcatagag ggagatacaa taaaaaaagt
    18661 atcaaatgat aagattgatt acgcatttga ctttaatgag atagatgaaa taatagatgc
    18721 aaaaaactta tatgtaatac ctggtattat agatattcac agtgatgcca ttgaaaagga
    18781 aatagagccg agaccaagta cattattacc atttaatatg gcattttatg aactagataa
    18841 aaaactacct gcaaatggaa taactacagt ttatcattct atatctttag gggatggagt
    18901 aggagttaga agtattgata actctttaaa gatgattaaa aatattgatt catataaaaa
    18961 tatagatagt aagagcataa atcataaagt tcatttaaga tatgaagtgt tgtattatga
    19021 aggtttagaa aaggttttag aactattaga tgaaaataaa atagactact tatcaataat
    19081 ggaccattct ccaggacaag gtcagtatac aaatccaaca ttttataaag agtatgctac
    19141 taaagtgtgg ggagttacag aaaattatgt agatacatgg ttagatgatt tagtaaatct
    19201 gcatgataat ttagattgga ataaaatagc taatattata ggtatagcca aaactaaaaa
    19261 tataaatgtt gcatcacatg atgatgatac attagaaaaa atggatttca taaaaaatat
    19321 aggcataaat gtatctgaat ttcctataac tcttgaagtt gcaaaacatt caaagaaatt
    19381 gggtgttcta acttgtttag gtgcaccgaa tattgtaaga ggtaaatctc ataataacaa
    19441 tttaaaagca atggatgcta tattggaaga ttgttgtgac attgtgtgtt cggattatct
    19501 tccatcagct atgataaagt ctatgtgtat agttgcagaa agaataaata atttaaataa
    19561 agcagtcagt ttatttacct caaatcctgc taaagcagtt ggtatatatg atgaaagagg
    19621 tagtataaaa gaaaataaaa aagcagattt agttttagta gatattgata gtgaatatcc
    19681 aaaggttgta aatactattg taaatggaaa aacagtttat aaaagggagg tataaagatt
    19741 gctaacagag ttacattgtc atacaagtat atcagataat aattttacta ctgaagaaat
    19801 aataaaaaaa gcagcagaaa agaatataaa tatcttagca ataacaaatc atgacacatt
    19861 atctggattg gatgaagctc ttgtgcttgg agagcattat ggaataacaa taataccagg
    19921 tatagaaatt tcagcctatg attataaaaa tagaaaaaga gttcatattc ttggatataa
    19981 catagattta aacagtcaag aaataaaaaa tttgtgcaat ccaatggtat gtgacagaca
    20041 taaagcatct gttaagatgg taaataaaat tatagattta ggatacaaaa tatctctgga
    20101 agatgtaaag aaatattcat caaagactgg aatatttaaa caacatataa tgaaagctct
    20161 tatagataaa ggatatacag ataatatata ttcaaatcta tataaagaat tgtttcatag
    20221 aggaaacggc aaggtatatg tatctttaaa atatgtggat tacagagatg caataaaagc
    20281 tataaaaaat tctggtggaa tttgtgtgtt agctcacccg ggtcaaatgg ataattttag
    20341 cgctattgaa gagatggtaa aagtaggatt agatggaata gaagtatatc atccatctca
    20401 caacaaagaa ctagaaaaag agtcgctttc ttatgctaag aagtataatc tagtggtcac
    20461 aggaggttca gattaccacg gattttattc tcaacataat tatgaacttg gaagcaagag
    20521 cttaaataaa gaagatttat ttaagtttag gttagcttta ggaggggtat tataaatgtc
    20581 ttttttacaa gtaaaaaatg taggtaaaag ttatggacaa gttaaggtgt taaaagacat
    20641 atctattgat attgaaaaag gagagtttat ttgtttacta ggtccaagtg gttgtggaaa
    20701 aagtacacta cttagaataa ttgcagggct tgaggataaa catggtggaa aaataataat
    20761 aaatgataag gatatgacta attcaccacc tgaaagtaga aattttggta tagtttttca
    20821 atcatatgca ttatttccaa atatgaatgt ttacaaaaac atagcctttg gactagaaaa
    20881 taaaaacata agtaaatcta atattgataa aaaagttaaa gaagttctag aagttgtaga
    20941 attaagtgga tatgagaaaa aatatccatc acaattatca ggtggacaac aacaaagggt
    21001 tgcattagct cgtgcgattg ctttagagcc agacttttta cttcttgatg aacctttatc
    21061 tgcattagat gcaaaagtaa gacttaaact tagggagcag attagaagtt tacatagaaa
    21121 actaggcatt acaactataa tggtaacgca tgaccaagaa gaagctcttt gtttagctga
    21181 taagatggtt gttatgaata gaggagaaat aatacaagtt ggaacgccaa aggaggtata
    21241 taaaaatcca gaaacacctt ttgtagctga ttttatagga acaataaatt ttatagatga
    21301 tggtattaat aagatagcta ttagaccaga agatataaaa gtagagtcta atagagattc
    21361 taaagataag gatataaaag tgggagaaat tttagatatc gaatttagag gatttaacta
    21421 tagaattaca gttgagtatc gttctaagca aatgaaatta gatgtagttt ccaaagttgc
    21481 agaacaaatg aaactgtgca taggttctaa aataaacttt aagataccta aagaaggaat
    21541 tgtacaatat aaatcagagg gatgtgctta atagctaggg gtgaataaat gataacatca
    21601 aaaaataaaa gaagaataag tgatagcaca atacaaaaat atttcttgat tttttcggta
    21661 attgtactaa tagtatttat attatttcca ttagtatcat tattaaaaaa tgcatttatg
    21721 gatacaagtg gaaattttat aggaatttca aactttagta aatatgttga aaatccaagt
    21781 cttatagcat catttaaaaa ttcaattttt gtatctacag tctcatcttt aatatcacta
    21841 tgtttagcat ttatatattc atacgcaata acaagaacaa acataaagtt taaagatttt
    21901 tttaaaacta tgggaatgtt tccacttttt gcaccaacta tgttatatgg tatttcactt
    21961 atataccttt ttggaaataa gggaatcttt acaaccatgg gtctggaagt accactgtat
    22021 ggtccattag gaataattat ttctcagatt ctatttacat ttccacaagc ttttttaata
    22081 ttatctgtag cactttcgat ggctgattac agactttatg aagcagcaga tagcttgggt
    22141 gctagtgaat ttaagaaatt ttgcaaaata acaattcctg gtataaagta ctcattactt
    22201 agttcattct ttgtatcctt tatacttgca tttacagatt ttggagcagc taaggtagtc
    22261 ggaggaaact acaatgtact ggctactgac atatataaac aagtagttgg acaatttaac
    22321 ataccaatgg gagctactgt ttctatggta atgttaattc ctgtactaat agcatttaca
    22381 ttagataaga tggcaagtaa gaaacaaggt atgaccataa ctgcaaagtc tatgccatat
    22441 aagattaagg aaaataagtt tagagataga gtatttttaa tagtatgtag ttttataaca
    22501 ttgctaattt ttattttaat attcgtttca gttataggtt cagttataaa gttatggcca
    22561 tataatttaa gctttacaat ggaacactat acatttaaag gtattttggg aagtggaata
    22621 aaaacatact taagcagttt gaaaatatca actttaacag caatatttgg aactatattt
    22681 gtatttttta gtgcttatat gatagaaaag gtggataagt ttcctaaatt aagacaaaca
    22741 gcttaccttt tatcaatgat accaatggcc ttacctggct tagttttagg tatatcatat
    22801 ataacttttt ttaactcagc aagtaatcca ttaaattttt tatatggaag tacagcaata
    22861 ttagttatag taaatgtagt tcatttttat tcagtttgtt ttataacctc aaattcatct
    22921 ttaaaaatgt tggataaaga gtatgaatta gttgcaaaat ccataaatat tccattttat
    22981 aaagtatttt ttaatataac agttccaatg tctataacat caatacttga aatagtagta
    23041 tattattttg taaattcaat ggttactgtt tcagcattaa tatttttata tacacctcaa
    23101 actcagactg catcaatatc aatactaaag ttagatgaga taggatatat aggaccatca
    23161 gctgctatgg cagtattagt gcttttaaca aatataatag taagactttt gtacgaattt
    23221 gtaacaaaga aattaaaaaa taacacacaa aaatggcagc aaaaagatgt tgcatagggg
    23281 aggtaaaaat gaaatttaag aagctagttt ctttaggagt attatcaaca cttttattaa
    23341 gtgtaataac aggatgtagt actgattcat caggttcaga ttcaaagtct gatagtaaga
    23401 caaaagggga tttaacagtt tacacagcaa ttgaagaaga tagtatagag ccatatttag
    23461 ctacatttaa agagaaatat ccagatatta aattaaatat tgttagggca tcaacaggag
    23521 atataacagc aagattatta gcagaaaagg ataatcctca agcagatgtt gtatggggag
    23581 ttgcagccac aagcttattg gttgcagatg accaggggat gttagaacca tatgctccaa
    23641 aaggttctga agaaatagag tctgcattta aagatgataa agaagttcct tcttgggttg
    23701 ggatagatgc ttggatgaca ggtataacag taaacactaa agaattaagt gataagaaca
    23761 tcccagttcc tagttcttac gaagatttaa taaagccaga atataaaggt cttatatcta
    23821 tgcctaatcc atcttcttct ggtacagggt acttaacagt atcagccctt atacaaatta
    23881 tgggggaaga aaaagcatgg cagtatatgg ataagttaca tgagaatatt ggtgtataca
    23941 ctcaatctgg ttcagctcca gctacatcag cagcttcagg agaatatcct attggtatat
    24001 cttttggata tagaggtata aagttaaaag aggaaggcta tccagtagaa gtagtatttc
    24061 caaaagaagg ttctggatgg gatatagaag caaatgcact tgtgaaaaaa gataatataa
    24121 aagaagcatc aaaagtattt ttagattggg ctataagtaa agatgcaatg aacgaatact
    24181 ctaagaacta tgcagttact actatttcta caggaaatcc aataccagaa ggattcccta
    24241 aaaagccatt agagcaaatg atagataatg atttaaaatc agcagcaaaa aatagagaag
    24301 atatactaaa taaatggata tcaaaatatg atggtaagac agaaaaagag agttaagtaa
    24361 aactatacaa atggagtaaa aagaaagaat ttttttaagt agattttatt ataataatat
    24421 aagttgagaa taatataggt caagaaaagt gaatattcta taaaaatgta tagagtaagt
    24481 ataccattca gtttgaatgg tatatttttt atttttagag attaatttag aaatagatat
    24541 actacagttt aaaatagtat attttaattt cattattaaa gtatagatat tatatataaa
    24601 gtaaataaat tattgagcga ttattaataa gatatgtata aattttaatt tgggaacttg
    24661 tgttatatat catgaaaaaa gagaaaaaat aaaaggtaaa ttgtttcttg ataacttgaa
    24721 attggaagtg agaatattta actagagaag aattatttaa gtttaagtca gctttaggag
    24781 gaggaattat aaatgtcttt tttacaagta aaaaatgtag gtaaaagtta tggacaagtt
    24841 aaggtgctaa aagacatatc tattgatatt gaaaaagggg agtttatttg cttattaggt
    24901 ccaagtggtt gtggtaaaag tacattgctt agaataattg caggacttga ggataaacat
    24961 gatggaaaaa taataataaa tgataaggat atgactaact caccacctga aagtagaaac
    25021 tttggtattg ttttccaatc atatgcacta tttccaaata tgaatgtata taaaaatata
    25081 gcctttggat tagaaaataa aaatataagt aaatctgata tagataagaa ggttagagat
    25141 attttagaag ttgtagaatt aagtggatat gataaaaaat atccatcaca attatcaggt
    25201 ggacaacaac aaagggttgc actagctcgt gcgattgctt tagagccaga ctttttgctt
    25261 cttgatgaac ctttatctgc attagatgca aaagtaagac ttaaacttag ggaacagatt
    25321 aaaagtttac atagaaaact aggcatcaca actataatgg taacacatga ccaagaggaa
    25381 gctctttgtt tagctgataa aatggttgtt atgaataatg gagaggtaat acagattgga
    25441 actcctaaag aagtctataa aagtccaaaa acaccttttg tagctgattt tataggaaca
    25501 ataaatttta tagatgatga tgtgaatatg atagcaatta gaccagaaga tataaaggta
    25561 gaatttaata gagactttaa agaaattgat gtaaaagtag gagagatttt ggatatagaa
    25621 tttagaggat tcaactatag aattacagtt gaatattgtt ctaagcaaat aaagttggac
    25681 gtagtttcaa aggtagtgga acaaatgaaa ctatgtgtag gttctaaaat aaacttcaag
    25741 atacctaaag aaagaaccat acaatataaa tcagagggat gtgcttaata gctagaggtg
    25801 gataaatgat aacaataaaa aataaaagaa gaataagtga tagtaagata caaaaatgtt
    25861 ttttagtttt tttagtgatt gcattagtag tatttatatt atttccatta atatcactat
    25921 taaaaaatgc atttatggat acaagtggaa gttttgtagg aatttcaaac tttagtaaat
    25981 atattgaaaa tcccactctt atagcatcat ttaaaaactc aatttttgta tctacaattt
    26041 catccttaat atcactatgt ttagcgttta tatattcata tgcaataaca agaacgaata
    26101 taaggtttaa aaactttttt aaaactatgg gcatatttcc gcttttcgca ccagctatgc
    26161 tatatggtat ttcacttata tacctttttg gaaacaaggg aatctttaca attataggca
    26221 tagaagtacc actatatggt ccattaggaa taattatttg tcaaatactg tttacatttc
    26281 cacaggcctt tttaatatta tctgtagcac tttcgatggc tgattataga ctttatgaag
    26341 cagcagatag cttaggggct agtgaattta agaagttctt caaaataaca attcctggta
    26401 taaagtattc attagttagt tcgttctttg tatcttttat acttgcattt acagattttg
    26461 gagcagcaaa gatagttgga ggaaactaca atgtattggc tactgacata tataaacaag
    26521 tggtaggtca atttaacata ccaatgggag ctactgtctc tatggtaatg ttaattcctg
    26581 tactaatagc atttacagta gataagatag caagtaaaaa gcaagggatg actataactg
    26641 caaagtctat gccatataag attaaggaaa ataagtttag agatagagta tttttaatag
    26701 catgtagttt tataacttta ttaatcttta ttttgatatt tgtttcagtt ataggctcag
    26761 ttataaaatt atggccatat gatttaagct ttacaatgga acactatacg tttaaaggta
    26821 tatcaggaaa tggaatcaaa acatatttaa gcagtttaaa aatatcaatt ttaacagcaa
    26881 tatttggaac tatatttgta ttttttagtg cttatatgat agaaaaagtg gataagtttc
    26941 ctagactgag acaaatatct tattttttat caatgatacc aatggcatta cctggtttgg
    27001 ctttaggtat atcgtatata acctttttca attcagcaag taatccatta aattttttat
    27061 atggaagtac aacaataatg gttatagcca atatagttca tttttattca gtttgcttta
    27121 taacttcaaa ttcatcttta aaaatgttgg atagagagta tgaattagtt gcaaaatcta
    27181 taaatattcc attttataag gtgttttttt atgtaacact tccaatgtcc ataacatcaa
    27241 tacttgaagt ggctgtatat tacttcgtaa attcaatggt tactgtttca gcattaatat
    27301 ttttatatat acctcaaact caaactgcat caatatcgat actaaagtta gaagagatag
    27361 ggtatatagg gccatcagct gctatggcag tattagtgtt tttaacaaat ataatagtaa
    27421 aacttttgta tgaattgaca acaaagaaat taaaaactaa gacacaaaaa tggcaacaaa
    27481 aagaagttgc atagaggagg taaaaatgaa atttaggaaa ttagtctctt taggaatatt
    27541 atcaacactt ttattgagtg taataactgg atgtaataat gcttcatcag gttcaaattt
    27601 aaagtctaat aataagataa aaggagagtt aacagtttat acagcaattg aagaagatag
    27661 tatagataca tatttaacta catttaaaga gaaatatcca gatattaaat tgaatgtact
    27721 tagattatca gtaggagata taacagcaag attattagca gaaaaagata atcctaaagc
    27781 agatgtgtta tggggggttg cagctacaag tttgataatt ttagatgaca aagaaatgct
    27841 agagccatac gctccgaaag gctttgataa ggtaaatcct gtatttaaag atgataagaa
    27901 agttccttct tgggttggaa tagatgcttg gatgactggt ataacagtaa atactaaaga
    27961 attaagtgat aagaacatac cagttcctag ttcttatgaa gatttaataa agccagaata
    28021 taaaggtctt atatctatgc ctaatccagc ttcttctgga acaggatatt taacagtatc
    28081 agaacttata caaattatgg gtgaagaaaa agcatggcag tatatggata aattgcataa
    28141 aaatattggt gtatacactc agtctggttc agcgccagct atattagcag cttcaggaga
    28201 atatcccatt ggtatatctt ttggaaatag aggtataaag ttaaaaggtg aaggttatcc
    28261 agtagaagta gtatttccaa aagaaggttc tggatgggag atagaatcaa atgcacttgt
    28321 gaaaaaagat aatataaaag aagcatcaaa agtattttta gattgggcta taagtaaaga
    28381 tgcaatgaat gagtattcta agaattttgc agttactaca attgacacgg gaaatccggt
    28441 gccagaagga tttccaaaga atccattaga acagatgata gataatgatt taaaatcagc
    28501 agcaagaaat agagaggcta tactaaataa atggatagaa aaatatgatg gaaagacaga
    28561 aaaagagagc taagccttgc aataaaattt agtacaaaaa gcgcctagaa tataagtttt
    28621 atatctaggc tcttttgata tatttattta aaatgcatat ttctatataa aaataaatag
    28681 tttagtacct tgattaaatt aaataaaaac cttaaaataa agagatgtaa agaaaaacta
    28741 aaaacgaata aaaggtgaat ttatatgata aaagaaataa tagtagtaga gggtagagat
    28801 gacgttacag cagttaagag agcaatagat gcagaactta taactacagg tggatttgga
    28861 tttccaaaag gtgtaatgga gcgtataaaa gctgctaatg aaagacgtgg tgttataata
    28921 tttacagacc cagattttgc aggtgaaaaa ataagaaaaa aaatagcatc agaagttcca
    28981 ggatgtaaac atgcatttct accgagagag gaagctaaaa aggatggaga tataggtatt
    29041 gagaatgcta ctcctcaaag tataatagca gctctgaata aagttagaac tgaaaataca
    29101 gaaaaaagag atgaatttaa gcaagttgac ttaattagaa atggtcttat aggtaatgaa
    29161 gaagcatcac ttagaagaga tgcacttgga aaaatacttg gaattggata tggaaatgca
    29221 aaacaatttt taaatagatt gaataattat ggagttgaaa gagaagaatt tatagatgca
    29281 ttaaaacaaa tctaaatgta atgtttaatg tatgaaaaat atgtatataa aataaattgt
    29341 ggaattacgg ttaatttaag ataaattatt tataatatat accttatttt aaatcaatgt
    29401 aaatacatat aaataagtat aattaataaa attaaatatc aaaataagtt aaaaaggagt
    29461 aaataatgga tagactatct tcacataatg caacaaagga agtagtacaa aaatataatt
    29521 ttaaattttc aaagtcttta ggtcaaaatt tcttgataga tagtaatata attgataaaa
    29581 tcttatctgg agctagaatc acaagaggag ataatatcat agaaatagga cctggtatag
    29641 gaactcttac tcgtgagatg ggtaaaatag cagaaaaagt agtggctata gaaatcgata
    29701 gaaatttaat tcctatattg aaagatacct tgtcagattt agataataca gaggttgtaa
    29761 atcaagatat actaaaagta gacatacagg aattagtaaa agataaatta aatggtgggc
    29821 cagttaagct tgtagcaaat cttccatatt atataactac tccaatagtt atgaaatttt
    29881 tagaggaaga catacctgta acagacatag tagtcatggt tcaaaaagaa gttgcagata
    29941 gaatgaatgc tattccgggt actaaagatt atggggcatt atctatagct gttcaatact
    30001 attgtgatac agagattgtg gcaaaagctc caagacatat gtttatacca caaccaaatg
    30061 tagattccac agttataggt ttacatgtaa gagataaaag aaaatatgat gttcataatg
    30121 aagacatatt ttttaaaaca gtaaaagcat cctttggaca aagaagaaaa actcttctta
    30181 attcgttggg aggtcttggt tttcttaata aagatgaaat aagagaaata ttaaaagaag
    30241 ctaatataga cgaaaaaaga aggggtgaaa ctcttagtat agaagagttt tcagttcttt
    30301 caaatataat aaatacaaaa gtatcttcta aatagaaggt acttttttta attttgttaa
    30361 catatattgt ttagagaaac tgtgttaagg ggggattgca gtgggttcta aaagaaagtt
    30421 taaaagagac tatataatac tagaagctaa agatataaac tttagataca aagaacgagt
    30481 actgccaaaa gcttttgcaa aagttgaagt aaatgatgaa aaagcaatta ttgcattata
    30541 tgtagaaaat ctaaaatatg ttaaagatgg gtataaagca gtagcaataa aagatgacca
    30601 tggattaata gacttaggaa atataatttt aaatgaacaa ggaagaggtg aatttgtttt
    30661 agatttagaa gattatgatg atgagataaa aggtattgca ttattgtatg aaagaaatat
    30721 acctttaatt ggatttaagg gaaataagct tgaaaattat gaggacatat tatttcaaga
    30781 ggtagaggaa gaagaatatg aagaatatga agagttggaa gaagaagacg aagagataga
    30841 ggataatgag gaaagtgata gagacgaaga tagtgaagaa gaatatatta tagaagaata
    30901 tgatgaagat gagtatgaag actattggga agatgatgaa gatagtgaag aaggagagtt
    30961 aaaagcaaga acggaagtag aagattctgg tgatgaggaa atagaagagt tcaataatta
    31021 taaggaaata gaaaattata atagggttaa agtacaggaa gaatatatag acatagaata
    31081 tgatgaggaa gaagaggaag aagaagtttt tgaagtaaat agagaagaag ataggtttgt
    31141 ttcctcaaaa cctactaaat caaagaaagt tcaaaaagat cagaataata ttgaaaaaat
    31201 aaaaagagct aatggagtaa gtcaaggaga gagtggaaat aaacaaacca gtacattact
    31261 tatgccaaga caaataaaaa aaggtttgaa attatttaaa gaagtaaaac cttttgtaac
    31321 agattatata gataagacaa gatggtggaa aatagagata aatcctacaa cactgtgtgg
    31381 atatacaatg ccttatttag gatatttaaa cgtattgaat tacactatgt atagtgatgc
    31441 agttttacat tcatataaat acagacacta tttatttgga gttcagtatg atgaatataa
    31501 taagagaaaa cattatattt atgctatccc aggaaataga aatgaacagc cagacaatgg
    31561 aagcacagga tttttaagat atcaagcttg tgataataga acaaatagtt taggatattg
    31621 gctatgtttt atagattcta gacaaagaaa tatagttagg taaaaataat aaaaatgaaa
    31681 aaatttagac tcaattctta ttttactgaa aaaattaatg aaatgtagta caaaacttta
    31741 aaaactattg ataatattta aatattacac tacaatatat cttgaaatac ctattatatt
    31801 atggaggaaa attatgataa acgagcaaag aattttaaat gagtttcttg aattagtaca
    31861 gatagatagt ttatctttaa aagaggggaa tgttgctaaa gtattagtta aaaaattaga
    31921 agaaataggt tgttcagtag taatagacaa tgctggtgaa aaagcaaatg gagaaactgg
    31981 taatataata gctacattaa aaggaaataa agaaggaaag aaaatattat ttagttcaca
    32041 tatggataca gtaactccag gtataggggt aaaacctata gtggatgaag caaatggaat
    32101 aataaaaagt gatggtacta caatattagg ttctgatgat aaagcaggta tagcagctat
    32161 attagaaggc ttaagatata taaatgaaaa taacatagaa catactgata tacaagttgt
    32221 attctctata tgtgaggaat gtggattagt tggagctaaa aatttagact atggaaaaat
    32281 agattctgaa tatgcattta tattagatag tggtggttct ccaggtgaaa taatagtaaa
    32341 agctcctgca caagatgtaa taaatgttaa ggtattagga aagactgctc atgcaggtct
    32401 tgaacctgaa gctggaataa gtgctataat ggtagctgcc agagcaatag aaaatatgaa
    32461 tttacttaga atagacgaag aaacaactgc taacatagga attataaacg gtggaacagc
    32521 tacaaatata gtaacaggag aagttaatat agttgcagaa gccagaagtc taaaagaaaa
    32581 taaattagat gttcaaacta aacacatggt agagactttt gaaaaagctg cgaaagattt
    32641 tggagctcaa attgagatag atgttaatag agcatacaca ccaatagatg ttagtgagga
    32701 ttctgaaata ataaaacttg caaagaaggc tttttcaaac ttaggtatag aaggtcatac
    32761 tgaatctaca ggaggaggta gtgatacaaa tatattaagt aagaatggta tcgaagctat
    32821 aactttagga attggtatga aaaatgctca tacactaagt gaacatatag ctataaaaga
    32881 cttatatgat tctgctaaaa tggtagtgga gataataaag gaagcataaa tttaaataat
    32941 agtgtataat atatatatcg cctagcttaa gctaggtgat ttttcatttt atagaagatt
    33001 gataatctct attgaaagca aagagtatac atttgcatat aagagagtgt ttaatttatg
    33061 ttcaaatgta ggttcttaat aatatttaga ttgtttcata agggaggaat aaatatggaa
    33121 acaaggtatg aagaagcaaa taaaataaca atacaatcaa tactttggaa tatagtatta
    33181 acaataatta aggttatagc tggtgttatt ggtaattcta gcgctatgat tgcggatgga
    33241 ttacattcag catcagatat tataagttct ataggagtac taataggcaa ttatgtatct
    33301 tctagacctg gagatagaga gcataattat ggtcatgaga aagcagagac attagtatca
    33361 tttgtgttat caatattgct tattttcgtt tctataacaa taggtataga ggcaattaaa
    33421 tctttattta acttagatgc attaagtgtt ccatcaatat taccattagt agtatcagtt
    33481 atttcaatcc tgataaaaga gtatcaatat agaataacaa taaaagttgc taaaaagata
    33541 aattcaccag ccctaaaagc tgatgcttgg catcacaggt cagatgcttt atcttcagta
    33601 gctgctttta taggtatagg tggttctatt ttagggttta aaccattaga cccaattgcg
    33661 tcagttgtag tggctatatt tgtagctaaa gtaggaatta gtatacttat aagttctgtt
    33721 aatgaattga tggatgtttc agttgatgaa gaagagataa aagaattaaa atttattgta
    33781 gcagatacag aaggtgttaa aaatcttggt gatataaaga ctagaaaaca tggagctatg
    33841 gcatatgtag atttgactat atgtgtggat gaaaatttga cagttaagca aggtcatgat
    33901 attgctacca agcttgaaaa acatataata aaacatatgg aatttgtaaa aggtataaca
    33961 gttcacgttg aaccatgtac taattgccaa ggaaataagt gtaataatta attaaaactt
    34021 aaaaaaaagt gtaaaaagtt atataattgt tttatgatta taaactctag gggtggttaa
    34081 atgagcaaga aaagaaaact aaagaaaaaa gctgcaatat taataggagt agttgttttc
    34141 tttataatat actttatatc ctttagaata ggaataaata tggaaaagga taaaagtgaa
    34201 gtgccaaaga aggtagaaaa agaagaagaa gataataggt ctttaaggac tcaaataaaa
    34261 gagaagaaga aggtttatat atcagataaa aatatagaaa acataaaaat tgaagaagat
    34321 atgtgggatg aaataaagtt atttttctca gattttaaga aagtaagaaa tactacttct
    34381 tcttacgaag cagtttatga aggttattct gatgatggag ttaaattttc tactgacctt
    34441 aactttttta gagtatatac tgtaaataaa gaagaatatt ataaagtacc agttagttct
    34501 aaggatgctt ttaagaaact tttagatgag agtatatata cttcgtttga ccttttaagt
    34561 cattataaag attggaaaga ggttactata actcatggaa aagaaattaa aaaagttcat
    34621 agatggaaat ttgatgattt agcacataag atgatttcaa agagggttgt aggaaaagtt
    34681 caacctgaga aaagtaagga aagaagtgac tataatttta ctataaatat aaaaggtgat
    34741 aattttgatg taaaagttga tacaatggga aaagattatg taaaagttac ttctaagaaa
    34801 aatgtatctt actatgaggt tcatacaagt ttatttgatt accttaaaaa tgagatattt
    34861 gaaattggaa agaaataaga ctttaagaag gctatcaaat tagatatttg atagcctaaa
    34921 ttattttata tattattttt caaccaacaa gtctgcaacc ttataaattg gaccagcacc
    34981 agcagttatt actaaatcat tatccttaac attttcacgt aagtatttta ctatatcttc
    35041 aaattctttt atatagatgg catctacatt gttttgatat aatttctcaa ccaagtcctt
    35101 agaatgtata tctcctgggt ctttttccct agcagcatat atatctgtta tgataacttt
    35161 atcagcagca tagaatgcct ctgaaaattc acctaaaaga gattttgttc tagtataagt
    35221 atgtggctgg aagatacacc ataaagttga tttttttaat tttttggcag cagctaaagt
    35281 tgcttttaac tcagttggat gatgtgcata atcatctact actaaagcat tcttatagta
    35341 ccctttagtt tcaaatcttc ttccgacacc tttataaatt tttatatttt ttcttatagt
    35401 ctctaagtca ataccagaaa caagagctgc cattatggca gaagaagcat tgtatatgtt
    35461 atgaagacca tatacagaaa gttcaaattc tcctaaatct cttccttcat attctattct
    35521 aaatattcca tgaccactat tgtcaaagtg aatatctttt attacagcat cattgtcaga
    35581 atctctgccg tattttataa tagtagcttt aacatcatac aatatgtcat caatattttc
    35641 atcatcgcca tttattatga aatagccttc tgggggtaat aattttccaa atttgttaaa
    35701 agaagccttg atttcatcta ttccagagaa ataatcaaga tggtcttctt caacatttaa
    35761 aactatagat atctttgggt taaaattaag gaaactatct acatattcac atgcttcagt
    35821 tataaagtgg ttggaattac ctatcttaac attaccacct atcatactta agttaccacc
    35881 aactagtata gttgggtcta agtctgcata ttcaaagatt gtagataaca tagatgttgt
    35941 agaagttttt ccatgtgttc cagaaactgc tatagaattt ttatattccc tcatgatttg
    36001 gcctaaaaaa gttgctctgt tcattaccaa tttatttttt tctttagcag ccacaagttc
    36061 ttcattgtct ggatgaactg ctgctgtata aacaaccata tttacatcat ctgatatatt
    36121 atcttttttc tgaccaatat aaatagttgc cccttggtcc ctaagtttgt ctaataggta
    36181 tgattcgttt gaatccgaac ctgaaacttg ataaccttta ttgatacata tttcagctaa
    36241 tgcactcatg ctgataccgc caattcctat aaagtgtata ttcataataa aaccaccttt
    36301 ctttttaatt aagtcctaca atatgaacta tttctgtggt ataataatat aatgttcaat
    36361 attttataat taatatgcat ataatattat atgtatttta tttttgaata taataaaaca
    36421 taagtaatat tataccacaa taagaattgt tatgcattag ttagtttaac gttatataaa
    36481 ggtacttgta ggaggaaata ataatgaaat ttaaaagaac agagagaatt ggagcaatag
    36541 ttaaaatact gtctgataat ccaaataaaa tatatacttt aagttatttt acaaatcaat
    36601 ttaatgcagc aaaatcaaca ataagtgaag atttattagt tgtaaaaaat gtatttgaga
    36661 agctacattt aggtaaagtt ataactattt caggagcagc aggaggagta aaatatatac
    36721 ctaaaacttc tatagcagaa aatgcagagt tcttaatgga attatgcgag agaatatgtg
    36781 acaagtctag aatactatct ggtggttttt tatacttgat tgacctaatt tacgacccta
    36841 caatagctgc taagatagga aagatattcg catcaaatat tgagtatgta gatgcagatt
    36901 atgttgtgac aatggaaaca aaaggtatac ctatggcact tatgactgca aaagctatga
    36961 atttacctct agttataata agaaaagata taaaggtatc agaaggtcct acacttagca
    37021 tgacatatgt gagtggaaac agttctaaag ttgaaagtat gagtttgcca agaaaagctc
    37081 ttaaaccaga tagtaaagtt ataattatag atgatttcat gaggggtgga ggtactataa
    37141 aaggaatggt agaccttatg aatgaatttg gtgcagaggt tataggtact ggagtattca
    37201 tatctacaac gaatccttca gaaaaaatgg taaaagatta tatttcattg attcaattgg
    37261 atgttaatgg agataaaata gtagtagaac caaacttaaa gacttttaaa gatgaatata
    37321 gaaatgaaga tttagacgaa gaagaagatt cagaatttga atttgagata gatgaagatt
    37381 aataaaaaaa atatttagaa aaatatgtaa aaataaaaag aggatatccc tagttgttca
    37441 tagaattatt taaaaaacag tttatattaa tttattccag cgataataat tttaatataa
    37501 attaatttct gtcaaaatca aagggggata ttttggtatg aagataactg acgtaagagt
    37561 aagaaaatta acagaagaag gtaagatgaa atgtatagtt tcaataactt tcgataattt
    37621 gtttgtagtg catgatataa aagttataga aggccataat gggctattta tagcaatgcc
    37681 tagcagaaaa gtaggcgaag gaaatttcag agatatagct catccaataa atgcagaaat
    37741 gagacaagtt ttagaagatg cagttttaca agcgtatcat gaagcattag ttcaatggga
    37801 agtagctgct gaataataat aaatatataa aatggagaga gcccgaacat ttaggctctt
    37861 tttaaatgta gaatttctat taaaatatgt actttttagt aaaaaagtgc tatattatta
    37921 gtgataatgt atatgttgta tacaaattgt tagataaatg tattataatg tatattgatt
    37981 aagagaataa tacgaataaa attaaattag attgaatgga gtgtatcaat gaactttaaa
    38041 gctataatcc ttgctgctgg caaaggaaca agaatgaagt ctaaataccc aaaagttata
    38101 cataaagtat gtggaaaaga aatggttaac catattatag atgtttcaaa aaaatcaggg
    38161 gtaaaagata ctgtcgtgat tttaggacat gaagctgatg tagttaaaga aaagcttgca
    38221 gaagaaatta taatagccat gcaaacagaa caacttggga caggacatgc tgtaaagatg
    38281 gctaaagaat atattaatga tgaagataca atagttgtac tttgtggaga tactccactt
    38341 ataaaagaag agacactaaa gagactattt gaatatcata tagaaaataa atatcatgct
    38401 acagttctta caacaagagt tggaaatcca acaggttatg gtagaataat aagagataag
    38461 aagggtgacc ttcttaaaat agtggaacaa aaagatgcaa attcagaaga aaagatgata
    38521 agtgagataa actctggaat ttattgtttt aatggaaaga gtcttagaga agcactagat
    38581 ttgctaaata ataacaattc acaaggtgaa tattatttaa ctgatactgc taaaataatg
    38641 agagataaag gattaaaagt tggtgcattt gcaggttcaa ctattgaaga acttatgggc
    38701 gttaattcaa gagtagaatt atcaaaggca gaagaaatta tgagacgaag aataaatgaa
    38761 tctcatatgg taaatggagt aactataata gacactaact ctacctatat agaatctgat
    38821 gttatgatag gcaatgatac tataatatat ccaggtgtta tgttacaagg aaaaacaagg
    38881 ataggttcag attgtataat tggaatgaat tcatctataa caaattcaga aataggtgat
    38941 ggtacagaaa taaaaaattc aactataatt gatagtaagg ttggagaaaa ttctactgtt
    39001 gggccatatg catacttaag accaaagagt gacttaggaa ataatgtaaa aataggcgat
    39061 tttgtagaag taaaaaatgc tattatagaa gatggctcaa aagcatctca cctttcttat
    39121 ataggagatg cacatgtcgg taaaaatgta aatattggat gtggagtagt atttgtaaac
    39181 tatgatggta aaaataaatt taaatcaata gtaaaagaca atgcgtttat aggttcaaat
    39241 tctaatttag ttgcaccagt agttgtagaa gaaaaaggat atatagcaac aggttcaaca
    39301 ataacacatg atgtcccaga tggtgctttg gcaatagcaa gagaaagaca agttatcaaa
    39361 gaaggatggg tagaaaaaaa gaatcaaaaa gacgaccaaa gtaagtaatt atgtagttta
    39421 ttattttggc taaaaaattt agctcgaaat tattataaat tatattcatt agtattttaa
    39481 aattttcagg agggttacat aaatgaatac tagcggaagc gagattaaaa ttattgcagg
    39541 taattcatcg aaagagttag cacaaaaaat agctgactat ataggtgtat cagtactaga
    39601 ttgtgaagta ggtacattta gtgatggtga aatatgtgta aacatgaatg agacagtaag
    39661 aggttgtgat gtgtttgtag tgcaatctac taacagccca gtaaatgata acttaatgga
    39721 gttattaatc ttaattgatg cgttaaagag agcatcagca ggtagaataa cagcagttat
    39781 tccttactat ggatatgcaa gacaagacag aaaggctaag gcaagagatc caatcactgc
    39841 taaattagtt gcaaatttaa taactgctgc tggagcagat agagtattaa caatggattt
    39901 acatgcagct caaatacaag gatattttga tataccatta gaccacttac taggtggaac
    39961 aatattagca aattatttta atgaaaagaa aatagaagat ttagtagtag tatctcctga
    40021 cttaggaagt gttactagat caagaaaatt tgctaatact ttaaatggag aagttccaat
    40081 agctataata gataaaagaa gaccgaaagc taatgtttgc gaagttatga acttaatagg
    40141 tgatgttaaa ggtaaaaatg ttatactatt agacgatatg atagatacag caggaacaat
    40201 agttaatgct gcaaatgcac ttaaagagtt tggagcaaaa gatgtttatg cttgttgtac
    40261 tcatggtgtg ttatcaggtc cagctattga aagaattgct aattcagaaa taagtgagtt
    40321 aatagttctt gatactatac aacttcctga agagaaaaga atagataaga taaagataaa
    40381 aacagtagct cctctatttg gagatgctat aagaatgata ttctctaatg aatcagtaag
    40441 taaattattc taatttattg tatttatgag agttgtttaa aaggatataa accttttgag
    40501 caactctttt ttgattttat acttatggaa tgttattttt ttcttgttag aaaggtatat
    40561 attattgtaa aaagagatta tagtttaaaa ttatgtatat ttaagtaaaa taaacctaat
    40621 aaagggtata ttattcaaaa tattatgaat ataaaaaata ttacaaaaaa gtattg
//
LOCUS       DAGSWI010000023        40091 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.023, whole genome shotgun sequence.
ACCESSION   DAGSWI010000023 DAGSWI010000000
VERSION     DAGSWI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.023"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(119..958)
                     /locus_tag="MJF80_001076"
     CDS             complement(119..958)
                     /locus_tag="MJF80_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBZ0279748.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMCILIVVGAITGIL
                     GLF"
     gene            complement(982..1740)
                     /locus_tag="MJF80_001077"
     CDS             complement(982..1740)
                     /locus_tag="MJF80_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBZ0279749.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1787..2260)
                     /locus_tag="MJF80_001078"
     CDS             complement(1787..2260)
                     /locus_tag="MJF80_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0279750.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2289..2705)
                     /locus_tag="MJF80_001079"
     CDS             complement(2289..2705)
                     /locus_tag="MJF80_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0279751.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2752..3804)
                     /locus_tag="MJF80_001080"
     CDS             complement(2752..3804)
                     /locus_tag="MJF80_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBZ0279752.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4200..4880
                     /locus_tag="MJF80_001081"
     CDS             4200..4880
                     /locus_tag="MJF80_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBZ0279753.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5013..5333)
                     /gene="sugE"
                     /locus_tag="MJF80_001082"
     CDS             complement(5013..5333)
                     /gene="sugE"
                     /locus_tag="MJF80_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBZ0279754.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5715..6542
                     /locus_tag="MJF80_001083"
     CDS             5715..6542
                     /locus_tag="MJF80_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBZ0279755.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGSKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7364..8038
                     /locus_tag="MJF80_001084"
     CDS             7364..8038
                     /locus_tag="MJF80_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0279756.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8026..9186
                     /locus_tag="MJF80_001085"
     CDS             8026..9186
                     /locus_tag="MJF80_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBZ0279757.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKNQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9403..9930
                     /locus_tag="MJF80_001086"
     CDS             9403..9930
                     /locus_tag="MJF80_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBZ0279758.1"
                     /translation="MKSFANNITFSRIVFALLMLSFTFCSLPFWICYFWCGISDILDG
                     LIARKLRQQSGIGAKLDSIADFIFSVTILVTVGKNIALPMYLWWCIIAIALLRLISYS
                     IGFFKYRTFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIM
                     IKSKELNRDIKSIFM"
     gene            10216..10884
                     /locus_tag="MJF80_001087"
     CDS             10216..10884
                     /locus_tag="MJF80_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279759.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKGYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            10887..13355
                     /locus_tag="MJF80_001088"
     CDS             10887..13355
                     /locus_tag="MJF80_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0279760.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYTLSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTNVSLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNILDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITNSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLTC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(13595..15019)
                     /locus_tag="MJF80_001089"
     CDS             complement(13595..15019)
                     /locus_tag="MJF80_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0279761.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15038..15730)
                     /locus_tag="MJF80_001090"
     CDS             complement(15038..15730)
                     /locus_tag="MJF80_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0279762.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15782..16561)
                     /locus_tag="MJF80_001091"
     CDS             complement(15782..16561)
                     /locus_tag="MJF80_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBZ0279763.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(16558..17328)
                     /locus_tag="MJF80_001092"
     CDS             complement(16558..17328)
                     /locus_tag="MJF80_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBZ0279764.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIFVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17325..18029)
                     /locus_tag="MJF80_001093"
     CDS             complement(17325..18029)
                     /locus_tag="MJF80_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBZ0279765.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDSNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18265..18777)
                     /locus_tag="MJF80_001094"
     CDS             complement(18265..18777)
                     /locus_tag="MJF80_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279766.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMDFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            19103..19360
                     /locus_tag="MJF80_001095"
     CDS             19103..19360
                     /locus_tag="MJF80_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279767.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            19503..20129
                     /locus_tag="MJF80_001096"
     CDS             19503..20129
                     /locus_tag="MJF80_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279768.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIEVSSINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20375..21154)
                     /locus_tag="MJF80_001097"
     CDS             complement(20375..21154)
                     /locus_tag="MJF80_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBZ0279769.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21222..21683)
                     /locus_tag="MJF80_001098"
     CDS             complement(21222..21683)
                     /locus_tag="MJF80_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBZ0279770.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21659..22336)
                     /locus_tag="MJF80_001099"
     CDS             complement(21659..22336)
                     /locus_tag="MJF80_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBZ0279771.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22569..22955
                     /locus_tag="MJF80_001100"
     CDS             22569..22955
                     /locus_tag="MJF80_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0279772.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            22958..24757
                     /locus_tag="MJF80_001101"
     CDS             22958..24757
                     /locus_tag="MJF80_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBZ0279773.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLVSWIIGILIMIAITIHCNIKIKNMKKSIQILRDDEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25112..26986)
                     /locus_tag="MJF80_001102"
     CDS             complement(25112..26986)
                     /locus_tag="MJF80_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBZ0279774.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVFPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVFSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(26991..28655)
                     /locus_tag="MJF80_001103"
     CDS             complement(26991..28655)
                     /locus_tag="MJF80_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBZ0279775.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(28669..29490)
                     /locus_tag="MJF80_001104"
     CDS             complement(28669..29490)
                     /locus_tag="MJF80_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBZ0279776.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(29589..30353)
                     /locus_tag="MJF80_001105"
     CDS             complement(29589..30353)
                     /locus_tag="MJF80_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBZ0279777.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(30350..31099)
                     /locus_tag="MJF80_001106"
     CDS             complement(30350..31099)
                     /locus_tag="MJF80_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBZ0279778.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(31603..32208)
                     /locus_tag="MJF80_001107"
     CDS             complement(31603..32208)
                     /locus_tag="MJF80_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBZ0279779.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
     gene            32387..33025
                     /locus_tag="MJF80_001108"
     CDS             32387..33025
                     /locus_tag="MJF80_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0279780.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            33157..33819
                     /locus_tag="MJF80_001109"
     CDS             33157..33819
                     /locus_tag="MJF80_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0279781.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            33819..35000
                     /locus_tag="MJF80_001110"
     CDS             33819..35000
                     /locus_tag="MJF80_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0279782.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(35181..37841)
                     /locus_tag="MJF80_001111"
     CDS             complement(35181..37841)
                     /locus_tag="MJF80_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0279783.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQVSVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(37838..38521)
                     /locus_tag="MJF80_001112"
     CDS             complement(37838..38521)
                     /locus_tag="MJF80_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279784.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(38865..39818)
                     /gene="blaCDD"
                     /locus_tag="MJF80_001113"
     CDS             complement(38865..39818)
                     /gene="blaCDD"
                     /locus_tag="MJF80_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBZ0279785.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT        14136 a         6554 c         4890 g        14511 t
ORIGIN      
        1 aaaatttaat ctattataaa attaccctaa ttgccaaact ttttaatata aattttccaa
       61 catcctacaa atataatcaa tagatataga atcctaactt acaccaatat atatttattt
      121 aaaatagtcc taaaatacct gtaatagctc ccactactat cagtatacac attattttta
      181 ttggagaaag accttttttt aaagcaaaga gtactaataa agtaagtacc aaaggaatta
      241 acccaggaaa tatcttatca agtaaatccg tttgaagact aaatttaaaa tcaccactag
      301 tataacttat tggtgtagat aaattaataa acctacctac aagtcccccc atgactaagg
      361 tacctagtat agaagcccct tctataacct tatttactat tccaccttga agtatacttt
      421 caacagcttt tttcccatac ttatatccat ttgtccacat tccaaaagaa attgaagtca
      481 ttattatata ttgagctatt acaaataata taggtcctgc aattgatgct cctccctcag
      541 ttaaaccaat acaaacagcc aacactatag gagttataac tccttgagtt aaagtatcgc
      601 caattcccgc tagtggaccc atcaagcctg ttttgatagc attcatacca tctccactta
      661 tttcatctgc tccattagct ttttcttctt ccatagctat tgttattcca tgtataacac
      721 atccacatat aggttctgtg ttaaaaaatg ctagatgagt ctccacttct tgttttaact
      781 cttccttatc tggatataat tttttaagta ctggcaacat tgaatgtgaa aaagctgttg
      841 attgcaatct ttcataattg taattagatt gtgcaaagaa aaaccatctt aaccatgatt
      901 ttattacatc tttcttgctt aacgttttct tagatacttc attttttata tctgacataa
      961 tattttcccc ctcacatgta tttaagatgt agcactatct tttccgtact taattgaagt
     1021 aattgtaaga gcaagtatta ctccaataac agatattact attatatcta ctttaaaata
     1081 aacagccatt agtattccta atatgaaaaa gggcatagta aacttgtttc caatcaattt
     1141 taaattcata gcaatcccaa gtgctggcaa catagcccca attacattaa atacatgtaa
     1201 cacattatca ttcattactt ccaacatacc atctatcgca tttggaccaa aataaacaac
     1261 taataatgct ggtataaaac tcattgcaaa taacagtatc tgtggtggta ctatatttat
     1321 aaatgctact ctatcaacat tccctttttg aacctctcta tcagcccaat gtgcaaaaaa
     1381 agaatttact gtcatctttc caatccaaag tacagttgca acagttccca atggtacagc
     1441 tattgccatt gcagtactta catctagctt agatgctata gctaaagcag tacctacata
     1501 tcctgcaaaa gctgggtccc ccatctgagc tccccctgct gatataaaac ctagatatat
     1561 catatttata cctgctccta taattgctcc ttgaacaggg tctcctaata taattcccgt
     1621 tacaaatcca gccaatagag gtctattcca tgtaaaaaat ccaacataag tcaaccacgg
     1681 actccaagaa agataataaa aaattgctat taatactgct tgaattagtg aaatgtgcat
     1741 attcttcctc cctattttct tttaaagatt tacaagatag tcttctttat aataatgttc
     1801 ctacatcaat tttctttgca tctggtacta tttgtataaa gcatttaaca tcttttgata
     1861 ttagctctct aaatatttct ttttcttcat ctgacacaga tatattttta taaaaacttt
     1921 ttctaccaag ccctgctccc atacctccaa cattcaattc ctttaaatct accccgctat
     1981 ttactaaacc taagacagtt ttagggtatt tgaccaatac tattaaattt tcatctccat
     2041 tatgtgattt taagacttca gcacctgatt ctacatcatg tacttcaacc ttaacagttg
     2101 atggagctgc catttgtaaa acttgaacta taaatgggtc ctttactact aaatcatcca
     2161 cgattactat cctttttgct gatgtaatct tacaccaagc agtaataact tgcccatgta
     2221 ttaatctatc atcaattctt gttaatgcta tattactcat aattattcct ccagattttt
     2281 aatttatttt acaactttaa aacagtttta acatctttga ttcctgatat cccaacttcc
     2341 tcacataaat tcttcaagtc ttttaacttc atagtatctc tgtttaaaaa taactctaaa
     2401 agcataggta aatttacacc tgtcaaacat tctaactcac ataccttagc aaatttattt
     2461 attataatag cagatgtatt aaatggagtt ccgccataaa aatcaagcaa tactaaaacc
     2521 ccttcatcct tatatttctc aattccatct tctaactttc tactaaactc atctatgctg
     2581 tcaccatggt ttaatcctaa agtttctaca ttttcctgtt tgcctactat taattcagca
     2641 ctttctaaca ttgctttagc catatttcca tgtgtggcta taattattcc tgccatatga
     2701 atcatacctt tcttaacctt ttttatatgg attattattt agtactctaa tctaattatc
     2761 tatattaatc attatcttac caaagaaaat atcccttttg tatacttttt caaaataagc
     2821 tggtacttct tctaaattta ttctatgtgt tactaatttt tcaacattca tgtcaccctt
     2881 ttgcatatga tacaatccag cactccattc ttttcctggg aatggaaagg agtttccaaa
     2941 ccaagttccc tttacagtta attcacttct tactatcttt tcaaattgct ctctagtcaa
     3001 agctacatcc ccatatggta cacctgcata caatactgta cctccctttt tagctagcaa
     3061 cagtacttga ccacatgtca gtggagtacc agccgattca ataacaatat ctgcaccatc
     3121 tccatcagtt aaccttttta tttcttctac aatattcttt tcttttgcat ttatacaaat
     3181 atctgcccct aactcttttg ccaagtctaa cttctcatca aatacatcca cagctattat
     3241 ttttgtagaa ccaaatattt ttgcccattg tattgaaaat aatcctattg gacctgtacc
     3301 aagtactaca acagtatctc caactttagc ttctgaccta aaaagaccat gtcctgctat
     3361 acatactggt tctagcgcag cggcagtctc ataacttatc tcatctggta tttttattaa
     3421 atttctctct ttcacctttg tatactctgc aaaacaacct ccaagttctt tatttcctat
     3481 tattgctaca ttattacatc ttgaatacaa gcctttttta cattcatcac attcaaaaca
     3541 tggcatagct ggacaaacag caactctatc tcctatttta aaactcctta cttctttacc
     3601 aacctgtgcc acttctccag aaaattcatg ccctaaaatt tctccaacca tatgtggacc
     3661 tgtctttgaa tattttgata tatcagaacc acatattcca gctactttta ctttaattat
     3721 tacatcatct ttttctaata tttttggtac atccacatcc tccactcttg tgtctcttat
     3781 accatagaat ctaactgact tcactattaa ccacccctac tttttatttg agaatatatc
     3841 cctattgttt atatgttaaa tatctaaagc aactttcatg ccaatatatg taagcattgt
     3901 attcatgcat tttataaaat tatcattaaa tgtgtgtcaa agtgtgtcaa aagtttctta
     3961 tattaaaact tatgtgtgtc aatataaacg acactaacat tttatattag ttctcttatt
     4021 ataagtttta taaaatgtac tttttttgtt atttttataa aattgttcgg aaatttccct
     4081 tctatttgtg atataatatt aaatataagg tatcaatata aattttcatg cgaatatcag
     4141 aacttttttg acactaatgc cttattcaat ataataattt aactttttgg aggttgttta
     4201 tgaaaaaagt gtatcaagga aagactaaag atgtttttga attagataat ggaaattact
     4261 tacttaaatt taaagacgat gtaactggta aagatggagt atttgaccca ggcgaaaata
     4321 gtgtaggtct aagtatagat ggaattggta gagctaattt agagacatct gttaagttct
     4381 ttgaaattct aaacaatgct ggaattaaaa ctcattatgt aagtgctaat ttagaagaag
     4441 caactatgga agttctacca gcaaaagttt ttggtaatgg attagaagtt atttgtcgtt
     4501 atagagctgt tggtagtttc ttacgtcgtt atggttcatg tgttgaagaa ggtgctaaac
     4561 tagattgtta cgtagaagca actttaaaag atgatgaccg ttgtgaccct cttataacat
     4621 ctgaaggttt agaagcttta aacattatga ctcaagctca atttgattca atgaaaagca
     4681 tgactcaaaa aatttctaac atagttcgcg atactatagc tgaaaaaggt cttgagcttt
     4741 atgatattaa atttgaattt ggattctaca atgatgaagt aatcttaata gatgaaatag
     4801 catctggaaa tatgcgtgtt tataaagatg gtgtaatagt tgaccctatg gatttaactg
     4861 ctcttttatt agataaatag tatatacata taatttacaa atataataaa cttataaata
     4921 agataagcta tgaagtaaag actttttaaa tgttgattat ttttaaacat tttctcgcct
     4981 ttaatttcat agcttattta aattaatata ctctaattag tagataatag ctttaaccct
     5041 acaattccca ccacaataaa acctatacat gcaattcttg atatactaat aggctcattt
     5101 aaaatagcca tgcctacaat agctgtaccg acggtaccaa ttccagtcca tattgcatat
     5161 gaagttccaa gtggaagatg ttttagtgct aatgacaaaa aataaaaact tacaatcatt
     5221 ccaacaacag taagtatact tggagttaat ttagaaaatc catgtgaatt ttttagttga
     5281 aaagcccaaa acacttcaaa caaacctgca acaaacaaaa ataaccattt cataaattca
     5341 tccctctttt caaatatgtc tcttattttt ccaataaaat aacctgagaa atattataaa
     5401 atatctccca ggtttttgtc cttccgtgtt cacaacaaaa actatgttgt gggttttatc
     5461 tcggaccaga ccaatcatta ataattgcgg aaccctataa aactctatta attattctat
     5521 tttatctata ataatactat caacatatat aaaaaaagtc aatatatggg ttccatatat
     5581 aataaaaata acttttatat accttttaat attcttattc acttctctcc atacccattt
     5641 gggtcaatct aagaatatct aatttttatt taatatgtta taataacata aaaatattga
     5701 aaggagtaat ttctatgaat ttctataaaa ttgcagtatg tcagatgatt actacagaaa
     5761 ataaaattga aaatatcaat catgctgtag atatggtaac agaagctgcc atcaatggat
     5821 ctaaaattgt agttttgcca gaaatgttta actgtccata tgaaaataaa tattttccta
     5881 aatttgctga agaatatcca ggtgaaacaa caacaatatt gagtaaatta gctgagaaac
     5941 atggaattta tctagtttct ggtagtattc ctgagttaga agatggaaaa atctataata
     6001 cttgctatgt atttgataaa aatggagctc taattggaaa acatcgtaaa atgcacttat
     6061 ttgacattga agttacagga aaagttagtt ttaaagaatc agatactctt acagctggaa
     6121 atgatgtcac tgtaatagat accgaatatg gaaaagttgg tatagctatt tgctatgata
     6181 ttcgtttccc tgagttatca cgtttaatgg ctcttaaagg tgctgaaatc gttatcttac
     6241 ctgctgcatt taatatgact actggtcccg cccattggga gttatctata agaatgcgtg
     6301 ctttagataa tcagatattc tatgttggtg ctgcacctgc tagaaatatg aatgcttctt
     6361 atatagcttt tggaaattca agaatttcag acccttgggg aagaataatt gctcaagctg
     6421 atgaaaaaga atgtattatt tatgctgaca ttgatagaga tttaattcct gacataagac
     6481 aacagttacc acttcttaag cacagaagaa ctgatttata tgaacttaat actttgaaat
     6541 aaaacagaac ataaccccat aaatcgtaaa ttaagtgtta aaaaaattta tggggttctc
     6601 attaatttga tttaaaatta atttcataaa aataatgcta aaattaattt aattaaataa
     6661 tactaataat tactaatgat tatgctaatt atcagcattt actaatttaa tcactattag
     6721 ttatttcttt tattaaatat ctttactttt ttacttatta tgtcttctag ctttacattt
     6781 ggattatggt atttctttct atttttagtc acatctttct attgaattac ttgcttaaaa
     6841 caccaatgca cacatgccag ataatgttca caattatgta gaaatcttgc tctaccttct
     6901 tcaatgttga tattatctct cctagttgat attatctttc ctaaaatttc ttttaattaa
     6961 aaaacaacat tagttgatat atcaagtata ttttaatcaa ctgaaaaaaa caataataat
     7021 tgatatatca actaattaat gtagattaat tcatttttat aatttatata ctttttaatt
     7081 aatctacctt atttaattaa aatccttcct ttctatttat ccattttcaa cttaaattat
     7141 aagatttcat cagctatatt tttgcatacc aataagtatt aaatatcaaa tatatattct
     7201 attaaaattt ctaataattt catccttgat ttatttaata tataaaaata tactaacttt
     7261 aatgattaag ttttgaaata taactaaaaa tttgataatt tattgagatt tttatactaa
     7321 aatatacata ctatacaaac ttaatttaga agggagcatt cttatgaaca ataaaatact
     7381 cgtcgtagat gatgaggaag gtataataac actcttaaaa gattactttg aaatgaatgg
     7441 ttatgaggtt tacactgctc aaagtggaaa agacgcatta aaaaaaatat ccaaaaatcc
     7501 agatatcatt ctacttgata taaacatgcc tgaaatagat ggaattgaag tttgtaatag
     7561 aattagagat tatgtatcat gccctattct atttcttaca gctagaattg aaaacactga
     7621 caagataaat ggttttcgtg ctggtgctga tgattatatt gtaaaacctt ttgatttaga
     7681 cgaacttgga gctagagtat ctgcccattt aagacgtgaa caaaggaagt caaatcaaac
     7741 taaaattaaa ttttataatg atttaacaat agattacgaa aaaaggttaa tttgtatata
     7801 taataatcag atttcacttt caaaaaagga atttgatata gtagagcttc tctctatgaa
     7861 tcctggacaa gtatttgata aagaaaaaat atatgataga gtctggggat acaatagcaa
     7921 tggaaatagt gatgttataa tggaacacat tagaaaaata cgaatcaaac tatcaaaaca
     7981 tactcttgaa aattatatag aaacagtctg gggagttggt tataaatgga taaaataaat
     8041 aatatgtcat taaaaaaagc tttctttgtt cttactttat cttcactaat tatagcatca
     8101 gtacttacta ctttcgcata cattttactt aataatctat ataattctat acaagataag
     8161 tatttaaaaa cacctataaa tacaggtatt ataaccatag ttcaaaacaa tgtgaaccaa
     8221 tttagtgatt atgataaaac attacttaat ataatcaaca tactccagct tgtacttcca
     8281 cttatatttt ttataggact tttattatta gctgatataa ttttttacaa ggtaaaatta
     8341 aaaacaccca tagaaatact caataaaggt gcaatggaaa tatccaacaa caatttagac
     8401 ttttgcttag aatataataa taatgatgaa ttaggtaatc tatgtaatgc ttttgaaaaa
     8461 atgaggtctg aactaaataa aaacaacata aagatgtgga ctatgattaa tgatagaaaa
     8521 caacttaatg ctgctttttc tcatgattta agaaatcccc taacagttct aaaaggttac
     8581 tcaaactatt taacaaaata cataccaaca ggtaagctta gtgatgagaa gattttatca
     8641 accacacaac ttatgtctga gcatattaac agaattgaat actatgtaga aaatatgagt
     8701 aatgctcaac gattagaaga tttagttgtt tcaaaatcta tgtcgaatat aaacaagttt
     8761 atagaaaact tagatgaaaa tatatctatc attgctaaac aagaaggaaa atcttttaca
     8821 cttaaaaatc aaataaataa tataaatcta ttttttgatg aaaatatcat tcttagagtc
     8881 gtcgaaaata taatatcgaa tgcttttaga tatgctagaa ataatgtatc catacttata
     8941 tatcttgaac aagaactcct tacctttgta attgaagatg atggtatagg atttagtaag
     9001 gaatctctaa aattagcttt aagacctttc tatagagata agactttaaa tgacagcaat
     9061 gcacactttg gtatgggtct ttatatctca aaaatattat gtgaaaaaca tggtggctct
     9121 atttctattg aaaataattc tactaacagt gctaaaatta ctgctaaatt ctcaacaaga
     9181 aattaaaatg attttgtaag ctttcactgc tattatataa aatttgttag tttagaattt
     9241 ataaaatgta tttcaattta taaccaatat atatactatt ttgtagaaat tgtaagagtt
     9301 gcttcaatta taatattttg gcaaaaggca tttgaaacaa taatagaaat atatatttaa
     9361 atatgtagaa tagtatgaaa ttagaatttt ggaggtgatt atattaaaag ttttgctaat
     9421 aatattacat tctctcgtat tgtgtttgca ttgctaatgc ttagttttac attctgttca
     9481 ctgcctttct ggatatgtta tttttggtgt ggaattagtg atatacttga tggtcttatt
     9541 gcccgaaagt tgagacaaca aagtggaata ggtgcaaagt tagatagtat tgctgatttc
     9601 attttttcgg ttacaatttt ggttactgtc ggaaaaaata ttgctcttcc catgtatcta
     9661 tggtggtgta ttattgcaat agcattgtta cggttgatta gttacagtat tggttttttt
     9721 aagtatcgca cattttcatc gcttcatact ttgatgaata aaatcactgg aatttctatc
     9781 tttgcgtttc cattactcta tatttgcttt ggtcttgatg ttgcaggaat tatgatatgt
     9841 atcgtggctt ttctttcttc ttttgaagaa atagcaatta tgataaaatc aaaagaatta
     9901 aatcgtgata taaaaagtat atttatgtga aaacttagaa tttgtgacaa aaataattta
     9961 tataataatg aggaagtaaa acataaaaaa ttatccacaa gtttatgtgt aaaatagttt
    10021 taaagcaata gagttgtatt gtaaagcctt tagtgcataa attacatttt aaattaaaaa
    10081 tgatactgaa actgcttatc agcactgcga attatccgca aatagtgaag actttttaga
    10141 tttaagctaa aagttgggaa aaaattgagt tttaaaactt acactcttct tatatagaaa
    10201 aggagagtgt tttttatgat tataaaagca aaacaattat caaaaatata tggttctaat
    10261 aacaataaag ttatcgcact taataatgta aatttagaaa taaactcagg tgaatttgtt
    10321 tctgtaattg gaccttctgg aagtggaaaa agcacacttt tacatatttt aagtggatta
    10381 gataatccta cctctggaca agtgttactt gatgataaag atatatataa acacactgaa
    10441 aaagaactat ctgcacttag aagaaaatcc tttggattcg tctttcaaca atttaatctt
    10501 ttacctgttc ttacagcatc tgaaaatatt tcaatgcctg tacttctaga taaaaaacaa
    10561 cctgataaag gctatttaaa tgaaatttca tcattacttg gaattgcaga tagactcaat
    10621 catcttcccc atgaactttc aggcggtcag caacaaagag ttgcaattgc tagagcactt
    10681 attgcaaaac cagatataat atttgctgat gagcctacag gaaacttgga cagtaaaagt
    10741 ggcagtgaag ttatgaatct tcttataaaa acttcaaaac agtttggaaa gactttagtg
    10801 gtaattactc atgatgatag aattgcaaaa cttgctgata gaaaaatttc aattattgat
    10861 ggtgttctta tggaggtgaa atagaaatga agaattacct ttcactttca ataaaagaat
    10921 taaaaagcca gaaacttatg actacattca tcattatagc aatagtacta tcaactatta
    10981 tgacaactgt ggttggacag tcaattggta tcctacaaaa tttaagaatt gaacaagccc
    11041 gttcttttaa tggtgataga catgtatcct ttcaccaact cactaaaaat caagttgatg
    11101 acataaagaa agatgataga gtttatcaag ctggtacttc aataactatt ggcagtagta
    11161 aaataaagga ttctggtatc tctgtacttg taaaagagta tgataaaaca ggactttcta
    11221 actacccaaa attaatgaaa ttaaaatctg gacatttgcc taaagataaa aacgaaatag
    11281 ccttagatga aaacacacta aagcttatgg gtataaaacc aagactagga gtgactatac
    11341 ctatgaattt agatatttca cttttaaatg ataccatacc accatataat tacacagcta
    11401 actttaaatt gagtggcatt ttagaggatg attatacagg atatgtaagt ggtatagtaa
    11461 atggtattgt aggtaaaggt acatcagaaa atctattacc taagagatat actttatcct
    11521 ctttggattt taaaataaaa cagcaagaaa agtttcaaga aatagtaaat caattagcta
    11581 aaaaaataaa tctttctcac aattcaattc aatataattg gatttactta aatgctctta
    11641 agatacagtt tgaaaaagat gaaaatagtt ctaatagtga tggtatatct atgattatat
    11701 tggtatcttt atttgttgcc ttattggttt tactggcttc tggacttgta atctataata
    11761 ttttaaaaat ctctgttaca aagaaaatta aagagtatgg atgtttaaga gcaataggag
    11821 ctgaaccaaa ccagatatat aagatagtta ttttacaaat cttaatttta tgtactgttg
    11881 ccatacctat tggagctgta attggaatta tatcttcgaa aggcataact ggaatggtca
    11941 ctaatatctt aaatccagat atacttctag caaatgataa taaagaaatt actgagctta
    12001 tacataaaaa tacaacagca tatatgttcc cattagtatt aagcactaat gtatctttga
    12061 ttttttcact tatttcagca ttaccatcag caatatatgc ttcacatgtc tctcccaaaa
    12121 ttgctatggc tggtagtact accaaaatca aaagaaaaat taaaagagaa aaaacaataa
    12181 aaaactttga aagacatcta gcatggctaa acttaaaaag aaataaagga agaactataa
    12241 taactatact atcattattt atgagtataa cagtattcgt tgcactgagt gagttctcta
    12301 atatattaga tgtatctagg tcagtaagta atttaaaaga aggagatttt tccttaacaa
    12361 atgaaatatc tggttttgat aaatcaacac tagataaaat agctaaaatg aaaaacgtca
    12421 atcgcacttc ctttataaag tactctgaat acaaacaggg tgaaatagat acagatataa
    12481 attttgaaaa tagtggtgag atgttaaaaa tcattggaat tgatgagcaa actttaaaag
    12541 acttaatgcc ttctattaca aattctatac tagaagactt taaaaatggt tctatctgtt
    12601 ttataaaaaa tccgattgct atttctacac caggtgtaaa aactagatat acaaatttaa
    12661 aacctaaaga tagtatcaca atcaacaaaa aacaactaga tatatatagt acagtagata
    12721 aaatgttctt tttacaaggc aatggctgga taaatggtgt tgatgttatt gtatatgatt
    12781 ctgtttataa cactttgaca aataaaaaca agttcaacca gataaatatt tatgctaaag
    12841 ataaatcaaa attagaacaa ataagattgt ctattgaaca gatttgtgaa aataatcctg
    12901 gctctcattg gatatcatat attgaatcag ataaacaact taaagagagt tttaaacaaa
    12961 tagaatttct agcatgggca gttatattat ttgtgggatt gattggtact ttaaacatca
    13021 ttaacacaac tcatacaaat ataaatacta ggactaatga gattggagtt aaaagagcta
    13081 ttggtatgag taataggagc ttatataaaa tgtttttatg ggaaggtgtt tattatggaa
    13141 tttttgctgc tatatttggt tcaattgctg ggtatgccag tgctattatt ataaacatgg
    13201 caactataga aaagcttgat ttcactaata taccaattac ttcaatttta caagctacta
    13261 ttatttctgt acttacatgt attatagcta ctcttattcc gttaagaaaa gttaaaaaga
    13321 tgaatataat tgactgtata gatatcaatt tataactatt ttacagataa taatataaat
    13381 taaattatca atgactgtct ctattttctt tcaaatctaa ctaataaatc tttttaacta
    13441 taatataagc tttaaaaaag gcctgtctca aaatagagat aagccttttt tcattgtcga
    13501 aataatgaaa atatatttaa cttcacaaaa aaagagtgcc tcacgaatta aaaatttcgc
    13561 tttgagacac tctcctttaa tttaaaatat atagttaaat gctatgccaa aatttcacac
    13621 atgctccacc tgattttgaa ttttctattt taacagaacc tccaagctgt tccactaatt
    13681 gtttgacaat gtaaagccca agaccagaat gtccaccttt tgtctgccta gcttcatctc
    13741 ctcgataaaa tttatcaaga gcctttttta aatctttaga gctaaaccct ctaccagaat
    13801 cacaaatctc ataagaaata caattttttt catcctttac agtaatacta atattaccac
    13861 ctgatggagt gtattgcaga ctgttggata tgatattatc aaaaattcgt gtcaacctat
    13921 ccacgtcgat ttgaattggg gattgtatat tcccctgcat tttcaatata agttcaactt
    13981 ccttttgatg agcctgtaat tcatagtctt gcaccttttg cctcaaaaat tcaggtaaat
    14041 ttataggaac aagatttaat tgtatatttg acttttctaa gtcagacgta tattgcatct
    14101 tttggacaag agcagcactt ttttctgtat tttctcttat cactgtaaga tacctatgaa
    14161 gtttttcatt gtcatctgta ttattaccaa taagtgcatc tgtatatccc aaaataatag
    14221 aaagaggaga ttttaaatca tgtgctagag cttccaccat ttcaacacgt tcctgctcca
    14281 tcttccactg tgcagataaa gagcctttta attcgtcttt catttcagaa aatgcactac
    14341 aaagtttacc gagttcatta tctgaataat agtcgatttc aaaatcaagg tccttttctt
    14401 ttatcttctt ggacgcatca atcaatagat gtaatggttg attgatattc tttacaaagc
    14461 gcttagaaag ccatctagta aaacccacga tatatagaaa tggagaaaac aaagccatta
    14521 taataactgc aaatacaaat ctccctctaa tatttgcaaa ggtgattttc acttggtaga
    14581 atagaataac agcaccctca atctttccat tatccccttt gattggaaca gtatggatat
    14641 aaccaccttt acgaacagtt ttattgataa aattattaaa taattcttct tttgttttaa
    14701 atagtttttt ggggttagta ccatataaaa tattgccatt attatccaca acttgatata
    14761 gcatatcatc cccacgaatc gttcttttta aaccttcttc attcgattgt gataacaaag
    14821 ctatgttttt ttcattgatg tatttttcaa ttctaggaac ttgtcgctca taatagtttg
    14881 gtggatagat atccttattt aaggaatgaa tatacaaata gagagcaaat acataagtaa
    14941 tcactgtggc aactatactg gctattataa taaaaataaa agtatgtcta aattgggtaa
    15001 taagggattg atttttcatt taataacctc tctctcttta cattttattc cacttatacc
    15061 caatacccca aactgtggaa atatattctt tttctggtgt cacagcagaa aacttagctc
    15121 gtattttttt tactcgttca acaacagttg aggaatcacc ttcagaatca cacccccata
    15181 ctttttcata gatttgttct cttgagaaaa cttgtcctgc atggagtgct agtaattcaa
    15241 caatatcata ctctattttt gttaaatcaa tattcttacc atcaatttta attgcacgtt
    15301 ccttcatata taagcataac ttcccaaaat acaaagtaga tctcttgttc tcttcattga
    15361 tatactgtga acgcttttcc ctacgcagat tagcttcaat ccttgccata agttcacgca
    15421 gtccaaatgg ctttgttaca taatcatccc ctccaaaggt caaccctttg attttgtcgg
    15481 tttctgattg ctttgcactt aaaaatataa ttgggcataa aacatcatct ctaattgcct
    15541 gacatacctc aaatccattc attttaggca tcataatatc cagtataata aggtcaggct
    15601 cctgttttga taactctata cctgcctgac catcataagc tatcaacact tcatgtcctt
    15661 ttgccttcaa ttcatcctca agtagcatta ctaggtccat ttcatcatca ataatcaata
    15721 ttttactcaa tataacacca cctttctctt atgggaaggt cagtattaaa acaatattcg
    15781 tctactcata acttttcctt ccctcccaca ttttaaacca aataacgcct cctatcaaga
    15841 atataatcaa acctattgct actactataa gctctatact agctgtttgc atcactttag
    15901 agtaaaatgc ctcagttgaa atagttccag cttctttaat aaaataaatt cctggaagca
    15961 tacctagttt tactggccat gtccatggaa caaatgccca tactttttca ccaacgctag
    16021 ttaatccaag tattgctgcc atcaaaattc caccaatact aataccaata gatgcaccca
    16081 taccccatgc aaaactcacc caaagatgaa tagctaatag tggaagtgtc ccaatcacca
    16141 caagtcctgc tgctgacagg aataaccata ttgttgctcc agatggcact gcaatactca
    16201 ttcctatgca aagtatcaac gtagcaataa atgtgcatat agttaggcaa aacagaagaa
    16261 aaagaaattt tcccagatat aatctgacac gagaaatacc tacacataaa aaaccattaa
    16321 aatttccagc aagctcttcc tcctgcacta taaatcctgc taaaatacct actccaatag
    16381 gaatgatgaa tacagtccat actgtaaaaa atccttcaaa agcaaattct tgtgtacttc
    16441 cagatttagt tgtgagataa aaaaccacac ataatgaaaa aataataggc atacaaagag
    16501 taagccactg gacagctgtc ctctttgctc gaagccattc tgatgataat agttttatca
    16561 tttgtcatcc ctcctattga accaagctgc agtaagtgct gttaatacta taaacacaac
    16621 caaagaaaca acaatcccaa taggaatcac tgaagtattc aaaagtgggt ctccagcttc
    16681 taatatagta ccgttaggat gtatgttgat gattgggcat accattctaa ctgcccaact
    16741 ccaaggtact gcaatccaaa atggttttgt cgcagcaaga acaccaaata tcatacctaa
    16801 agctccaact cccatactta ggaacatacc tttccatgta gctagccata gctgaagtgg
    16861 aagtatagca agagaaacaa cccagcatac aatacttgcc cctataatct gtccaaaagg
    16921 aactggacca gctttcaata ttaaaccagt tataattgtt acgaatatta aaactagcgt
    16981 tgaaataaaa ctgtagactg ccatagctat aactttatta atccaaagtg tcataggaga
    17041 cacattgtgt gtacgtaaag cacgataatt tccagctttt ttttcctgga aagctaccaa
    17101 agtagcaaac aatgcaaatc caataggcag aaataaaaat gaccaccagt taaaaattaa
    17161 tgctaaaaga ttttgccacg cccaagaagt tgtttgagat aatggatttt gcatgagtgt
    17221 tgattgcatt ataaatgcat atgctgcaaa agacactggg aaaaaaacaa taagtttacc
    17281 cataaatgtt cttttatact ttaaaagttc tgattgtaaa gcattcatct gttttgtcct
    17341 cctttaagaa taacatctgt aaaaaattct tctagatttt cattagagtc aggcattcct
    17401 tgaaataata gtcgtcctcc atttataatt cctatattat ctacaacttg agcaacctct
    17461 gatagaatat ggctagaaag aattacagtt ataccttttt ttggaaaaga agcaatcaat
    17521 tcacgcaatt cttgaatgcc aaaggggtca agaccattgg ttggttcatc tagtattaac
    17581 aactctgggt cactcaaaag tgcaatagct attcctaacc tttgtttcat acccatagaa
    17641 aattgtgaag ctctcttttt acctgtattt ttcaaattta ctatttccaa cacttcataa
    17701 attttatctt taggtattcc caatattttt gtatgtacta acaaattttc ctctgctgta
    17761 aggtttccat acaatgcagg ggattctatt aaagaaccta tttttgcaag agattctcgt
    17821 ttccaaggct ttctatcaaa aataatacga ccatttgttg gacgaagaag accaacaagc
    17881 attttaagtg ttgtggactt tcctgctcca tttggtccta aaagtccata tatactattt
    17941 cgttcaattt gtagagaaac attgttaact gccatttgtt ctccatatct ttttgataag
    18001 ttttttgttt caagaattaa atctttcata ttaatttctc ccttcttgta ataccttaaa
    18061 tctgttacta agagaatact aacatgaaaa aataacaatt ctataacacc tactaatttt
    18121 aataaaattt aaaataaaaa atagagttac caattttatt ttggcaactc tatttaagtt
    18181 tatgttttac agttatatta gatttaaact tagtttcatt atcacactaa cttcaaattt
    18241 actctataat ctttaaatca aacactaatc tattaattta tatttcttta gacatctaat
    18301 atagtttgct actaaataat aattatttat atcatattta tctaatcctt taaaagaagt
    18361 tatttcatca ttaagctcat cactaccttc tacaaaaaca caacctgttt caaagtccat
    18421 acctacaata atcttacagc cactataaat ttcctcttga gtatcgtaca atttttttgc
    18481 atttccttca tttggaattg taaatacaat aggttttaat tctttatcac tggaaatctt
    18541 ttgtgaaaaa ggcttgttta acttaatatt attgataact acatgtttaa attcctctga
    18601 agtttcttct actattacat attttcgctt taaaaactct attatttcta aaacagactt
    18661 agaatttact tttaaaatac ttttatattt ttcatataaa gatttccact gttcaatttt
    18721 ttcctgtggt acttcttgga tatgtgtact tattttttct tgagatattt ttttcaatta
    18781 aactctcctt tcaatactta aatatcctta taaatctcac ataactaaag ttacaaggtt
    18841 cctacttaat agaattttgc atacttaaat atgtcttatt aattctctac aggatatctc
    18901 cgtagttcct acgattctta cataagttac ttattttaaa ttgttattag ttatattcta
    18961 aaaattattt attttatcac taatataata ctcttaaaaa ctttacaaat caatttaaat
    19021 ttgctacctt ttatttataa caacttatag tacatttctc ctatgctttt caatatatta
    19081 cttttaaaag gaggttttca ttatgatttt aaatagagct gagcttttcc gaggatattc
    19141 tttaaataga tttaacgaga tatgcaatat attatcagta aataaaatta aatataagta
    19201 taaagtgaaa aataatgctt actctaaaaa accttttttt aatgaaataa cacttggcaa
    19261 tttaggtcaa aaggaagatt tctcttatga gtacttaatt tatgttcata aagacgatta
    19321 tgaacaggca gagtctctaa taaattctaa gttgcgatag attatttata aaacatgaca
    19381 ttctgttgtc atgttttata tgttattata ctttcaaata tgctactata ttttcaaaat
    19441 tgtgtgcttt aaaactagct ttttatagta atttaaagct taaaactaag gagactcgtt
    19501 atatgaaaat aaatataaaa tcaaagttaa gtgaatttat taaacagaac aatctttttg
    19561 atgacagcag aataatatcc taccttccta atataacaat agaaacagac aaaataaaaa
    19621 ctataatgat taatgaggtt gtcccaacta acaccaatga cgatttttat agtgtagata
    19681 aagatgctga ttatttaaaa actactattc ctttatttga cagtgcagga atagaagtat
    19741 ctagcataaa tgatatccta gatatgggta tctatataac taatgcagta aaacttccta
    19801 aatcagaata tacaataact cgtgatacaa ttaaattaca tatgcctatt ttagaagaag
    19861 aaattaaatt atttaaaaac ctagaggtag taatgttaat gggagatgtt gcaaaaaaat
    19921 cttttaatat gattactaaa aagcatataa aaaagaatgt catcccttcc atttcaacat
    19981 ataaacttcg taataatgaa ctttattatg ataatttaag agtatttcca tcatatatta
    20041 tgactggtgg aaacatactg attgaaaaat caaaatttga aatggcatca gaagatataa
    20101 aaaaaatgtt cgagattata aacggttgat attacaaaat aataaaaact aatataaaat
    20161 aaaagtgcat caaaaacttc tatttatgag gcacttttat tttatattag tgtaactata
    20221 tactattaac ataatttatt atttatattg ttctttttaa tctctattcc atttagtcta
    20281 ggtaacatta ctctttacaa gattacttta tatttctttt atatctctaa cgtttttatt
    20341 taataaaaca attattaata caagtcaaat atatctattt atctaaaaat atatctttta
    20401 cagtaatatc tggatggtga tacttcttcc tattcgttaa aaaagatttt tttacattaa
    20461 tagcctcttt tggacaccag tgaaaacaag ctaggcatct ttcacactca ttaccccact
    20521 ttggcttttt atcaactata cttatattct ctacaggaca aaccctttta catgtcccac
    20581 atccattaca tttattttct gcaaaaaatt tagtagttgc aatttgcata ttcttaccta
    20641 ctaaacgaag tacaacacta tctatatgtg actttaaatt gttttttcct tctatatttc
    20701 caatctccaa gttattgata cattttgcaa tctcagctac cctattagtc tgatttttca
    20761 atctttcttc ttcaacatct ggaggtgtta tcaaagcatt tccaggcata ttgattaaaa
    20821 aaccagcaga taattttttc cccctttttt taagtaactt atctatagta aataaactat
    20881 gtccagctac cccattacaa gtggcaatgg catatatatg agtattaata ttaaaattta
    20941 atttctctac aaaagacttt actatatcag gaacatcagc aaaataaaca ggaaaaacaa
    21001 ttaccaatat atcttcattt atgtctatat tattttcttt tctatgtata gctaagggta
    21061 taacttcttt acttccattt aacgtttctt ttaaaatatt tgaaactact agcgaatttc
    21121 ctgttccaga aaaataataa atcttactat tcattcttat ccctctttct atatttctac
    21181 atcatcaata gttaaaaagt ctcttaaaat aggatttata tttactttcc aattctatcc
    21241 aaaatatcag taaacaaagg caattcttca tcagagatag atttaaatgt ctctgttgca
    21301 tatatacttg caatttcatt taatggttct tctaattctc tacctaaatc tgttaggaat
    21361 atttgaaact tacgtctatc tttaacatct actttacgga caattaatcc cttatttttc
    21421 ataacatcta agattcttgt cgtattagat ttatccttac atgatttttc acataaatct
    21481 atctgagaaa ttccatcttg ctcccataga aaccttaata acgcataatg gtcagttgtt
    21541 atatcatatc cattttgttt aaacatcttt tttaaatcat cttttacttt taacgaagta
    21601 cttactaact tagttgtagc ttcatatagt ttctcaaaat ctttcatttt acctccattt
    21661 aaataatccc ttgctcctcc catctatcaa ttattccctt atattgctca atatctttaa
    21721 aagccattgg ctttaaaaca gcatttatag caataaaatt atagatttga gaaagcacag
    21781 gtggattttt cttttcctct atgcaataaa taaatttttt agataccttt tgtatctttg
    21841 attctacttt ttcagttata ggattttgat taaatccagt ttttatagat aaactaccac
    21901 aaacataacc tcccgaatct aagaccatgg attttataaa agaaattgct tttgaaccac
    21961 gtgcattttc atatgtagta actgctatac atggcttacg atacaataat ctttcaagca
    22021 tcatatgtgc tctatctgta aacaccttga aaagtccagt tacactactt ccataagttg
    22081 ggctaccaaa tataacacca tcactcttag taattatatc atgaatatac tctactttat
    22141 cattttgatt gatacacttg cccattttat aacaatttaa acaaccaata caatatgatg
    22201 gattgacctc acttaaatca aaataattaa tttttacatc tggtattaat ctttctatat
    22261 ctttatacaa gtaagtcaaa acttttgatg tagctccatt ttttctaggg cttccattta
    22321 taactgtaat tatcatttct atatctcctt tttagttgtt atatcaacaa taagtatatc
    22381 aactatattt ttagctgtca atataaacca tttatttaaa gctatactta cacagcttaa
    22441 ataaaagttt tatttaacta catgtgctta tttaatcaac tataaatgaa ttatttttaa
    22501 taattgacaa actaaattaa tgataatata ctttatgtat tacaaatgta agtagaagga
    22561 ggtatagtat gaaaaagtta ccacatattt cagaagcaga atatcaagtt atgaaaatta
    22621 tttggaaata tgctcctatc agcactactg aagtaattga aaagttggta gaaacaagta
    22681 catggagtcc taaaacaatt caaacaatgt tattgagatt agttaaaaaa ggagctttaa
    22741 catatgaaaa aaatagcaga gtattcgttt atactccact tgttaaagaa gaagaatacg
    22801 tagctacaga aagtagttct tttttaaatc gtttttataa tggtactctt aattctatgg
    22861 tactaaactt tttagaaaat gataaactat ctgaagatga tattgaggaa ctgagagaaa
    22921 tcttaaataa aaggacgact aagggggata aataaatatg tatgccccat tctttatacg
    22981 atttttactc agtacattaa ccttatcaat attaattgtt gtaattttac ttgccaaaaa
    23041 agtatttaaa aaacatattt ctgcaaagta ccaatacaat attggattta tattgcttat
    23101 aatgttaata attccatttg tccctcttaa atatattaat cttggaaatg tatttaacta
    23161 tttatatact tttaatcaac ttggaagcag tactaatagg acttctatga taaaaaatag
    23221 tccaaattct ataattaata actcaaatgc attaaacgat ttttcagtat ccctaaacca
    23281 agttaatttt gatagtttaa atattttatt actagtatca tggataattg gaattttaat
    23341 catgatagct attactattc attgcaacat taaaataaaa aatatgaaaa aatcaataca
    23401 aattttaaga gatgatgaga tagtatttat atttgaaaag tgtaaaaaag atattggtat
    23461 aaataaggat tttatactat gcaaatcacc tattataaat acacctatta catttggata
    23521 ctttaaacca cacattatat taccagataa gtctatatct aaattatctt taaaagatat
    23581 aaaatatatc ttacttcatg aattacaaca ttttaaaaat aaagatatac tgataaatta
    23641 tataatgtgc tttcttcaaa tattatattg gtttaatcct cttgtatggt atgcatttaa
    23701 agaaatgcgt atagaccgtg aaattgcatg tgatatttca gttttaaaaa agcttgataa
    23761 agatagtcat atagaatatg gacaaactct aataaacttt gtggataata tttcaagacc
    23821 atcaaatctt acacttacat cagatattgg tgggtcaaaa aaacaaataa aaaagcgact
    23881 attgaagata atggattttc ataatgaatc aaatacgtta aaattcaaaa gcattttaat
    23941 ttttacattg atagctttaa ttgtatttgg aaatattccc aaactatcta ttatggcaga
    24001 tatcaacgaa aaatatagac ctacaaatga acaaactgtg tacgaggact taagtcagta
    24061 ttttaaaggt tttgatggaa gttttgttat gtatgattta aaatccaagc aatatcatat
    24121 ttacaataaa gaaaaaagta aaacaagaat ttctccaaat tctacttata agatttatag
    24181 tgccttattg ggattggaaa atgggataat aacaagaaat aattcacata tcccttggga
    24241 tggaaagcat cattctaata gttcttggaa tgaagaccaa gacttatttt ctgctatgga
    24301 aaactccgta aactggtact ttcagactct agataaaaaa acaggtctaa aaaacataca
    24361 aacttatctt aggcaaattg gatatggcaa ttatgatgtt tcaggtggta tctcaaactt
    24421 ttggctagaa tcttcattaa aaatatcacc tattgaacag gtagaattac ttgaaagttt
    24481 ttatacaaat aagtttaaat ttgaagataa aaatatagaa acagtaaaag attcattgtt
    24541 aatatcaaaa aaggatgggg tatcactttt tggtaaaaca ggcactgcca atataaatgg
    24601 aaaagatgta aatggttggt ttataggtta tgtggaaaaa aatgaaaata cttatttctt
    24661 tgctacaaat attcaaagca aagattattc taatggtagt tcagcatcta agataacatt
    24721 atccatatta aatgacaaaa aaatctataa tccttaatat atttactgta cattctaaaa
    24781 aattcacata attttcatat taattatcaa ttaaattcat atacaaggct tattatataa
    24841 acatagaaga tagtaaaact agcttaaaaa cataatataa taccctatac aaatagtggc
    24901 aactctccct cacatcctca attgttgcca ctatttaatt ttttgtatga agttaaatat
    24961 attttcatca tttcaacaat ttgggtgcaa tacaatctct attttgaaac aacctcttta
    25021 attgttttag agtttatgaa ttatttctga tactttaaaa agattgaatt actcttgata
    25081 atcttctttt gtagtcatta catatcacct actactcaac acatattctt tgacctattt
    25141 ttacatcctt gtttatatcc aaaatattta ccttcttacc atctggaaac aataccattg
    25201 tcaaagtaga ctttcctaat tcgtgtattc tctttaaatc aacattaaca agaacatctc
    25261 cctgtttaac catatctcct tgtttaacag caacatcaaa gccatctcca tttaatgcaa
    25321 ctgtatcaat tccaacatga attaatactt ctccattagt tcctttaatc ccaaatgcat
    25381 gtccacttga aaatactgtt tctaatttac ctgaaatagg agaaacaatt tttccatcta
    25441 taatatcaat agcaaaacca tcacctaaca tttttttaga aaatgtttca tctggtacat
    25501 tttctaatgc aacaattttc cccttagctg gaaacacaaa gttttcatta ttttcattaa
    25561 acactaattc ttcgtcttca tcttcattta tagcttcttt aggcactcca aataaacatg
    25621 ttacaataaa gcagaatgca accacaaata ttgatactaa taagtatgtg attaaattat
    25681 atccatcata gatatacatt aaataagaag gtaaaactgc taatccatat gaattagatt
    25741 gaatatgtaa aattgataat attgctgctc ctaaacctga agatgcacac ataataatca
    25801 aaggctttaa attatatcga agctgaatcc caaataaaac aggctcactt ataccaaaaa
    25861 gttgagatag cattgctcca accgcagttt gtttaacttg attattttta gattttttca
    25921 taaatgctag acatgttcca atattcgcaa atccatacat tgcacaaaga gtaataaggg
    25981 cattaaatcc tgtatttgct aatagtgatg tttcaatcat tacataagta tgatgtaagc
    26041 cagtaataac agctaatgga tatgtaaatc caataataaa tcctccaatt cctaaaggca
    26101 aatcaatcaa taagctgatg atatcaacca tttttaattc tattgtatgc attatagggc
    26161 ctacacctaa caaaataact aacattgtta ttagcataac aaaaaatggt gtgaaaataa
    26221 ggtctaatac attaggcata tgtttacgga accatttttc aagattagct ccaataaatg
    26281 ctgtaacaat agctgttaaa acacttcctt gacaacctac aataggaata aatccaaatg
    26341 ccataattgc ttcaatttca tttgatgggt cagcaacaga atatgcatta ggaagtattg
    26401 gggaaactaa cataagtcca ataacaagtc caataacagg tgttcctcca aatactttaa
    26461 atgctgacca tacaataatt gctggtaaaa aagcaaatgc tgtttctgta agaaccgaaa
    26521 caagtgtctg aatatacaat ggaatatttg cagaagacat tccaaataat ccaagtacat
    26581 tatcattaaa caaacaaccc tttaatccca agaataatcc tgttgctgca ataactggaa
    26641 taataggaac aaatatatct gctagagttc tcataacttt cttaaatcca gtttcattat
    26701 ttttaactaa ttcatcttgt tctttttttg ataaagtttt taatcccatt ttttcaactt
    26761 ctgcataaac tttattaact attcctgttc ctaaaataat ttgatactgt ccagaagtaa
    26821 agaaaacccc tttaacctca tcaacttttt caactttctt atcattaata atttctctat
    26881 ctttaaccat cactcttaat cttgtggcac aatgtgtaat agaaataata ttatcttcac
    26941 caataatttt aataagctca tttgctattt tattatattt attcattttt ctacctcact
    27001 ttcaataata ctgcatgaaa tggtttcaaa cgcactgttt tatttaatag atttatatca
    27061 tctaaatttt ggaatacaat atcatatttt tcttctactc ttatttctac aggtttatca
    27121 gaaaaattat aataacaatc aataactacc aaatctaatt gccttctata cgcaataata
    27181 tcatcacttt ctatatcaat aggttctatt gaaccataaa tcaaacaatc agaatactca
    27241 ctatcttgtc tcaactttat catcttttta taaaattcta aaatactctc tgaattgtta
    27301 atctgactct gtgcattaat atctttataa ttttcaataa cctttaacca tggtgttgta
    27361 tctgaaaaac ctgcattttc tttatctgcc catggaaatg gcgttcttgc attatctcgg
    27421 cttcttaaat tcacaaattc taacgcttct tcttctgagt aactttcatc tattgaacga
    27481 tagtattggt caatgctaga taaatcatta aaatcattta tggtatctct tttaaaattt
    27541 gtcattccta attcctgtcc ctgataaata aatggggttc ctcttaaaaa gaaatacatt
    27601 gctcccaaca tttttactgc atcatcattc ctttcgtatt gcaaaagata ctttgttgta
    27661 gctcttggtt ggtcatgatt ttcaataaaa ttagctgccc atccatgttc ctgaacatac
    27721 atttgactat ctaaaatgca ttttcttaaa tctggaattg tccatgaaat tcttttaaac
    27781 cactcactac cagaagcaac atctaagtca gcatgtttaa agtcaaagac cattgaaaag
    27841 aaaccatctt ctccaataaa atcacctagt tggtcatagc taatacctgc tgcttcagca
    27901 acagtcatac aatcatattt tttaaacgta ttttctttta attcataaag cattttttct
    27961 attccaggct gatttctaac tgtttttgta catttaacaa gaccatcaac accatcactt
    28021 tctaaacttt gaaaacttaa attctttttg ataaatgtaa tagcatcaac tctaaatcct
    28081 gccactccca tttgagacca ggtattaatc atttggtaaa gttcacttct taatattgga
    28141 ttttcccaat taaggtcagg ttgtttttta ccaaaagaat gaaaataata ttcgtctcta
    28201 ttctctacct tttcccaaac acttccacca aaaacagacc tccagttatt tggtaaatcg
    28261 tttccttttt taaaaatata ataatcatga taaggacttt ctggattttt taatgcttct
    28321 tgaaaccaag gatgttcatc acttgtatga ttaagaacta aatctaatat aattttgatt
    28381 cctctatctt tactttcttt aattaactct tttaaatctt ccatacttcc aaattcactt
    28441 gcaatatcca aataatcact aatatcatac ccattatcgt ccataggtga tttaaaaatt
    28501 ggacataacc acaatgatgt aatacctaaa tcttttaaat aatctaaacg agaaataatt
    28561 ccctgtaaat ctccaattcc atcattatta ctatcttgaa agcttcttgg ataaatttga
    28621 taaacgattt ctttttgcca ccatttcttt tccataaatt actccttttt atatgatatt
    28681 tcttcttttt aattctttat aaattccatt atcgaatatg tcctcacata tagatgtcgc
    28741 tatatcttta agttgttgat gactattttt cattgcaatt gtaacgtctg atgcttgaaa
    28801 catgacaatg tcattttgac catcaccaaa acatatggtt tctttttgag ttactcctaa
    28861 tctttcttgt aatctcttaa ttgctttgcc cttatgaaaa tctttttgaa taatttcata
    28921 atactgacta gaaatatctc tctgtgctaa ttccatttta tcttgtagta tatctttcac
    28981 ttcgtcaaat accttttcat ttgaccataa acaaatttta tgtatatctt gtgacttata
    29041 ctcttcaatg ttattttcat atgttatttt ttcttggata tgttgtttat ttatacaaga
    29101 attagttcct tttaactgat tcattgtttc aaatatttct ttggcttttt gattcatgaa
    29161 aactttttcc tgactctcaa tcgaaaaagc aacttctctc ttttttaata aacaaacaac
    29221 ttcctttatc aatctttgat taaaagattg attataaagt aattcaccat gatattgaat
    29281 gtaattacct cctcctgcaa tatatccatc aactcctaat gataaaacat catcttgtat
    29341 tgtacccata cttcttccag tacaaataac aacactgcaa tgattctttt gacataaacg
    29401 tatagcatgc tttgcagatt ctgggattcc atatacctca tctctaagcg taccatctat
    29461 atcaagtatt aataatttgt acaatttcat cttcctttct tactatcaaa atcataatag
    29521 cattcaaaaa aaaagatatc aatattttga tatcttttaa aaaatatgtt catttttagc
    29581 acaaattttc attttcgggg aatatgttca ctttctaaat acatcttgta tttaatataa
    29641 agcagttcaa ttaaaataaa ataaggtgtt gttacatcgt agtccagttg tttatctaca
    29701 gaaatagata tagattgaat atacaggttt tcatcacata atcttgctaa aggattactt
    29761 atcatttttg taatagaaac catataaatt cctttcattc tcatttcttt agcaaaatca
    29821 ataattgtag atgcatctcc attcaatgaa ataaagaaaa tcatatcatc tgtttttata
    29881 aaatgattaa gtgcctgaat catatcataa ccataaacat catatataat tttccctgtt
    29941 ggtaagaaaa tccttttcat ctctttagca actctttcct gagaataacc ttctccaaag
    30001 acgattatac gatttgcctt tttcattctt tcaaacataa tcgaacaatc cttcttctct
    30061 atatcatcta tcaccttatt ataacaatcc attaattggt gtttactttg atttttatat
    30121 tctacttctg tttcatttaa gcgtataata actcttaatt cactatatcc aggtaattgt
    30181 agtttttgag caaaacgaga aagtgcactt gtagatatat ggtatttgtc tgcaaattca
    30241 tctatagaaa gtttaataca atcttcttta tgattaagaa tacactcaca gatatactta
    30301 tcatttgctg taaatgaatt ataatattta ttgaaaagtc cttcaatttt cattcttttc
    30361 tccttctata ctctatgtaa ttcatatgaa gcaaatctaa tagagcataa aatgctccta
    30421 cgacttcata agatatagct tgtaatgtat caagtttttg cgtttcaaca tacagattat
    30481 actcacaaag tgttgcaatt gtattattat caagcctagt taaagaaatc gtatgtatat
    30541 gattgttgat aaattttgca atttcaattc ctgcttttgt ttctccagac aaagaaatga
    30601 taataaataa atcatttttt tgaaatgatt ctctcataaa ttcaatttca cctatatcaa
    30661 ataagtcaat aacacattta cctactgaaa gaaatattct ctttatttct tgagctaaac
    30721 ttttctgttc attacctgtc ccataaatat aaattgtttc tgcatcatat attattttac
    30781 aaatatcttg aaatgatatt ttttgtaatt cattcattaa tagtcgataa ttctcgcaaa
    30841 catcatgaaa attattatca ttattaacaa attctagttg attattttta agtaaatact
    30901 ttaaatcaga gaatgaatct aatccaattt ttctacaaag ccttaataaa gtagctctag
    30961 aaacattaca ttcatttgca attttttcac acgaaaaatt acggtattct ctttgacaaa
    31021 tcaatatttg tttaataata attttatcat tttcatttaa attatcataa ttttggttaa
    31081 ctaacgattc taatctcatt ttttccactc cctaaatatt attttaatat acattagttg
    31141 ctttttctag ggaagagttt taaataataa ggataaaata atcatttgat tttattttta
    31201 ttaaaataaa aataacctgc taaaagcagg caccaaaaag tacttatcct ttcgaacatg
    31261 tactttagat attgctttct tcggaagctt catctcttca tactctaaaa tatagttttt
    31321 tctaactcaa aagtattttc catggtagaa attactttaa ttcatctgcc atttactttt
    31381 tatcttattt aataattact attacttgaa tatttacttc tttaagtaaa tttcatagtt
    31441 aacaaagcta aaactattat tatagatgtt aaaatacaaa attgtaagta ttgttataaa
    31501 tatgcttaca gctacaaata tacatgatat attaaaaaga agctgtaaat tattatcata
    31561 catacaagca actatgcctc tattaatcca tgttaatttt tattatttaa tacaaggagt
    31621 tccatgagac atacatatcc attcatattt taaattatcc aattctctta tagatttcac
    31681 catcttttta taatcccaat tccatggatt aggataagga actatatttc cattcttatt
    31741 tttgactaaa tcacctgcaa ataatacagc ttcaaaaatc atgcagacat gccctggagt
    31801 atgaccagga gtatggataa tttttatatt cccaatctgc atattctctc cataaggttc
    31861 aacatcctta atgagccttc tggaaattgc ctttccaata aacttcttaa acccaggtct
    31921 atccttctct cctataataa aaggtatatc ctctacatga gcccatactt ttgctcctgt
    31981 tttttctttc aataatttaa tattagatat atggtcaaca tcatgatgag ttaataaaat
    32041 atgcttaata tctgtaagtt taatgccaag tgtttttaat tctttaatta tatttttccc
    32101 cataaaagga agacctgtgt ctataagaat aatttcttta tcaaacacaa taaaacaatt
    32161 tgagccacga gtactatcta acattaatac attttctttt atcttcataa ataaattccc
    32221 cttatttggt attttaccta tataattaat taataaagtt ataaatagtt ataatacttg
    32281 atattaattt tattttttat taaagtatac tataggtata caagtattat atttacataa
    32341 gtataccatc agtatacttt tttatcaata gaaaggaata atttatatgt cagaattatc
    32401 aaaaagagat aaagaaaaaa tacaaagaga aaatgagatt attgataagg cagaaaaatt
    32461 attttgtctt aatggatttg ataatacaac tatgaatgaa ttagcaaaag aagttgaata
    32521 tactaaacgc accatataca agtacttttc atgtaaagaa gatttattct ttgctgttgt
    32581 cttaagagga tataaaagac tttgggataa tgttaaaata gaaagtgcaa aaggcaaaac
    32641 agggtttgaa aaaataaaac tttcatactt tgcttttcat aaattttact ctgtagaacc
    32701 ttctctatta tgccttatgg gaatgattgg tattgtaaac tctgaaaaat cggatactga
    32761 tatgctgttt aaagaaaagt ttttttcttt taataaattt atgtttgatg aaatacaagg
    32821 aatgtttgaa attggaaaaa atgatagaag tattaggcat gatattgaaa tacctactct
    32881 tatgtattca tcaatattta cacttacagg attttttaat ttactatctg taacaggaaa
    32941 atcatacctg aacaacttta atatagatga ggaaaaattt attgaaacaa ctcttgctct
    33001 tttaatagat tcaattaaag cataaaccta agtttataaa ttaaagttta tatacttaaa
    33061 tgataatttt ttctatctct aatataaaaa atattaaaca taaaactaat aacttagtta
    33121 atggaataac taaattactg aaaggattaa aagtaaatgg ctaaaatact tcttttagaa
    33181 gatgatgata caattacatt tggaataaaa acttcccttg aaaagaaaaa tcacactgta
    33241 tgttgttata atacgattgc tgaagctaaa aaaaattttg ataaaactat tgatttaatt
    33301 ctgcttgatt taaatctacc tgatggtatt ggatacgagt tttgccaata tgttaaaagt
    33361 atagaagata ctccagtgat atttttgaca gtccgtgatg atgaaaaaga cattatacgt
    33421 ggattagata tgggtgcaga tgactatgtg gttaaaccgt ttttgctctc agtacttcag
    33481 tccagaatta atgcagttct tagaagaact cattcaaata ctaacaatac tattacatgt
    33541 gaaaatataa gcttgaataa ggttgaagtc aaagcatatt tagataatgt tgaagtacct
    33601 ttgacagcag gagaatataa acttcttcta agtcttatgg aaaataaaaa tagagctatg
    33661 actcgtacaa agttactaga aatgctctgg gatgtagatg gtaatttcgt aaatgataac
    33721 actcttactg ttacaataaa acgtcttaga gaaaaattaa aaaatccacc ttgtataaaa
    33781 acgttaagag gcataggata tagaatggag gacagctagt gaaaactcca agaaatatca
    33841 acagtacagt tgtttttata atattctgct taattgtaac ttttttattg agtgtaattc
    33901 taacaactat aacttctttt aatgaataca atgtactgtc taaggctctt ggtgcatcat
    33961 atgagtacaa tcctaagtct acagaaagta tactaaaatc actgaaaaat tctagtgata
    34021 aggattttaa aacaggaaat gaaattttat caaaacatgg atattctaaa agtacatttt
    34081 ggagtaaaaa ctttttttat tttctagtga tatcgtttgg tataatttca acaatggcta
    34141 cagttatata tataatcaaa catagaacaa ataagcaaaa actgagtaga ataaacaatc
    34201 ttactcaata cttagaagaa gtgaatttag gaaaagagtc tgtactttta cgatgtgaag
    34261 atgaattttc tcaccttgaa gatgagatat ataaaacagt tggggaactt cgttgttcaa
    34321 aagaatttgc tttaaaagaa agacaaactc tttcagataa tcttgcagat attgctcatc
    34381 aacttaagac tccaattact tctatgtcac ttatggcaca attactttca gaaaattgtc
    34441 cagatgaaga aatagattat attaacagac ttacaaatca aatctcaaga cttgagagac
    34501 tagtttcatc tttacttaca ttatcaaaat tggatgcaag tactttaata tttgaaagca
    34561 aacttataga tgtacattct cttcttactt atgcaattga gcctatagaa ggttctttaa
    34621 gagaaaaaca tcagacattt actatcgagg cttctccttc ggtagaattt gaaatcgata
    34681 ttaattggac tttagaagct cttttaaata ttcttaaaaa ttgtagtgag catatagaag
    34741 atggtggata tatagttgct tattatagtc aaaatcctct atatgttgag attatcatag
    34801 aagataatgg taaggggttt gctcaggaag aacttccata tatttttaat agattttatc
    34861 gtggtaaaaa tgctagtaag gacagtattg gtatagggct tgctctatca aagtctatta
    34921 tagaacgtca aaatggtact ctacatgcag aaaattgcca taatggtggt gcaagattca
    34981 taattaaatt ttacacataa aatgctggat tataggtaaa atacctataa tccagctata
    35041 tctatattct atgttatcta ttgttttagt aatatacttc tatttacatt taaggtttta
    35101 ttaaacaaac aacttaacta tccctatact tgtaagtagt cttaaatcga aacctttaaa
    35161 taagttcaaa tagattttgt ttatattgtt tcatttttta tagattctac tatattttca
    35221 ttcttcaact gcttgtatcc tgtcatatat gatgctatta caataaaaag tatcaatata
    35281 gtaaaaccta ttattgtaga tataggtgca aatggtaaat actctataaa atatatttca
    35341 tttatacgta gaaaagcata cactattcct atttgcacag gaatactaag tagtaatggt
    35401 gtaattccta agaataaacc ttccaaaata agcatctttt ttatgccttc tggagataat
    35461 ccaacagaac gcaacatagc aaactcttgt cgtctacttc taatatttcc tgacactgta
    35521 gcaagtacat ttgataatcc aatcatagcc aataaacctg taataaagcc tactattaaa
    35581 ttcatggtct ttattccact agccttttgt ttttcacttt ctaaaacatc ctcaatacta
    35641 taatctcctg aaccatagtc tttattataa atggactcta atttttctct tactctagga
    35701 atgtcctcac gattatttac tttaaatact cctatcagcc tatttgaacg taacctactt
    35761 acttcatcat aatttttaga tatttcctgt accatagaca ttggaactat ttgcattaat
    35821 gtatactttg aactagtatc tactatttta ggaagtgtgc ttgtaatagc tcccactttc
    35881 atgttgtaag tataatcccc tttatcttcg tcatatactt tttcatttaa tttaatgtta
    35941 tctcctattg aaagatttaa ataatctata tatactttat ttctacgtgt actacgatta
    36001 atatcttcct ccttattata tacaattgag gttggatttt ttgtattata aaataacttt
    36061 gggtctacag aaacttgcct acaatattct gtaaaactct tatcatcaag cccaattata
    36121 tttgaaatta tacgaaactt gccatcacgt tctataggag agtatttttt agatgaaact
    36181 atacttttaa atcctccatt tttcgcaagt tcttttgatg catctttttc agtcagccaa
    36241 gtggaagcag acattttttg ataatatagt gaacttttta caccttctgt attatctaat
    36301 ttttcaatga aagcagactc tacaggttca ctattttcca aataaactgc tatatctctt
    36361 tggtccttat aattatcact tccataaaca gcttttgctc cttcttgatt tgcaattata
    36421 tgcaaaaaac atgtaagtaa taaaaaagat aatgttaaag atattgttgc agtacgataa
    36481 gattttttac gagcacgtag tgcgtttgat gcaagttctc cttctattcc aaatatcttt
    36541 cctaatgaat atttatgcga ctttttgatt ttaacactat ctccttgtcg tattgcttct
    36601 ataggagata tctttgatac ttttcgtgct ggtataagtg cagaaatcca gactgttact
    36661 attgtaagta aaactgctgg cagaaaagca ggaataccat aagtaaatac cacttctgga
    36721 acatcagtat gataattaac tgaattaaca taaactataa gcctattaca caataaccat
    36781 cctaaaaata aacctattgg taatggtata attgtcagta aaaagccttc aaacactaca
    36841 gaccttttaa tttgttttgg tgatgcacca acactagata atatccctaa ctgagtaagt
    36901 ctagcatttg cagacagtga aaacgcgttg taaatcacca ttacaaacac ggctactgta
    36961 aaaattgcta ttgtaagata cataatagga gttgaaaatt ttgatatact actcatagat
    37021 gctttctgtt ctggtgaaaa gactaacatc tttaaaagat aattactatt gtatttaaga
    37081 ttgtattttc catattcatt agtcttccat cccaatgatt tagcaagttt tggaagttcc
    37141 ttatacgtgt ctcttatatt tttaaatcgc aagtacacag ttatactatc attaggtttt
    37201 atacttgttt tatcaagata tccaagagct gtatatgcag gtattactga tgaagtagaa
    37261 acatctacct ttcctactat tgtcaatgta gcctctgaaa tttttttaaa actttcccct
    37321 ttcgattttg gggctatggg ttcaagaggc tttccattta agattctata tcctattggg
    37381 agagtaattt tatcaccaat ttttacactt ggattattct caaaatattg ttttgataat
    37441 gcaatttcat tagcattatt tggtcgacga ccctctatta tagtatcctt ttctggcata
    37501 gaattccaat actctgatgt agcatttcta taaattatat aatctctttt ctcatcatta
    37561 attttgccta cataccattg tccttttacc attacatcat caactgaaga aaaattttca
    37621 attaatggta agtcacttcc ataagtatag tcaaacaatt caccatgcca gttaccttct
    37681 ttctcaattg aaagtcttat aaaatctgtc cacatggtat acaacagtcc acacaaagaa
    37741 gacatcatgg ttgtagtaag taatatagct aacattatag ctatactact acgtttattg
    37801 cgtcgtacaa agtctaaggt gtattcttga agaatattca tttcttcacc accttttcag
    37861 agacaatcac tccatcttcc atagttacaa tacgatttgc ctctagtgct attttctcat
    37921 catgagtaat aagaagtatt gtctgattaa atcgtttatt tgataagttt aacaagtcta
    37981 caatttcttc tgaatttttt ctatcaagat taccagttgg ctcatctgct aataaaatag
    38041 ctggtctata gattaaactt ctagctatag ataccctttg ttgttgtcca cctgaaagtt
    38101 gacttggaag atggtttaat ctatcagata atcccaatat agatacaatc tcagagaatc
    38161 tatcctcatc cacctttctt ttatcaagta acatgggtaa taatatattt tttctaacat
    38221 caagggtagg tataagatta taaaattgat aaataagacc tacttttcta cgtctaaaaa
    38281 ctgcaagagc ttcttccttt aaacttgata tttctgtatc ttcaacataa atctttccag
    38341 atgtaggtct atctactccc ccaagcagat gtaacaatgt agatttgcca gaaccagatg
    38401 ctcctacaat ggatacaaaa tcaccttttt gaactgataa atttacatta ttaagggctg
    38461 tcactctggt ttgatttgaa ccatatattt ttgtgagatt ttcacatttt aatatttcca
    38521 tatacaatca tctcctttat ggttaaattt tagcaaccta aaatgacatt atagtgacat
    38581 gaatacaaat ataaattact attaaaaata ttgtttagtt tttgcacatt atttaaaatt
    38641 ataatataaa acaaaaacta tgcaatatat tttatttata cttttaatat attacatagt
    38701 ctttaaaagg tattaacttt aaatctatag gtattcaatt taataatcaa tagcaagtat
    38761 tttatgtact aaaggagttt tgctctatat agactcaatt tagtaaaatt atttaataaa
    38821 tattacagac tataggtaaa taatatttac aaataaaatt aagtttattc ccttacacta
    38881 taatatttct taattatatt aattgcaatt tcctttacct ttggccctgt aatttcttta
    38941 ttggaatcat ctgatttaat agcaaaataa taggtgtcac cttcacgctc tagcatacca
    39001 acaaaccatg catccacacg tttatttttt tcatcaaatc ctgttccagt ttttccatat
    39061 acatttatgt ttttatcatt tatatctatt ttcataatat ctcttaaaat attaatatgc
    39121 tcttttttaa agtttgtatc tccctcaaat atttttgcca ttgtttgcac ttgttctttt
    39181 ggagatattt gtaatgaaga ttctaaccaa aatccattta aatgtccctt tccatttttt
    39241 aaatcgcctt cccattcgct aatatcgcaa ttcccatact ttaaatcatc taaaatgttt
    39301 tggacagatt tagcatcaac tttatctatc aattttttgt aataccaaac acaagactcc
    39361 ttaaatgcct cttctaaact aagattttta ttccaatttt tatttggata ctctgtacca
    39421 tcatatccca taactgattc ttttgagttt attacccctt tttctaaacc aattaaagtt
    39481 gaaactattt taaaagtaga acatggtgac cttcttgttt caattaattc tttgttatat
    39541 atattatatt ctttattttt agtattataa aaaatagctc ctccactaat accttcaaaa
    39601 caatcactat agtcaaccat attaattttt tcttttttgt ttttatcatt gttttcaatt
    39661 ttggatttat ttaattcaga cttatttaat tcagatttta tataattctt atcagttttt
    39721 tgatttatat cgttatgttt tttccctaca taatacatta ccataactac accaaccaat
    39781 aagatagcta tccaaataaa attctttttt cttttcataa tttatatcat ctcccatatt
    39841 taaatttata acctactatt aaaatagtag acattatttg taatatactc caatctaggt
    39901 tctacttata agaaacaaga ctaaatgaat agctgtatct cttatatttt cttcacctag
    39961 tacaattatg ttgattaaac ctattgcgaa gataattagg taactattaa taacatcctg
    40021 tcctcctttt aatttgtgat ataataattg attccacatt taaaacttac tattgtaata
    40081 tttaaatatt a
//
LOCUS       DAGSWI010000024        38257 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.024, whole genome shotgun sequence.
ACCESSION   DAGSWI010000024 DAGSWI010000000
VERSION     DAGSWI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.024"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1866)
                     /locus_tag="MJF80_001114"
     CDS             complement(<1..1866)
                     /locus_tag="MJF80_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279786.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGEDFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFC"
     gene            complement(2049..3524)
                     /gene="spoIVA"
                     /locus_tag="MJF80_001115"
     CDS             complement(2049..3524)
                     /gene="spoIVA"
                     /locus_tag="MJF80_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBZ0279787.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(3842..4861)
                     /locus_tag="MJF80_001116"
     CDS             complement(3842..4861)
                     /locus_tag="MJF80_001116"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBZ0279788.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(4892..5506)
                     /gene="plsY"
                     /locus_tag="MJF80_001117"
     CDS             complement(4892..5506)
                     /gene="plsY"
                     /locus_tag="MJF80_001117"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBZ0279789.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(5541..6860)
                     /gene="der"
                     /locus_tag="MJF80_001118"
     CDS             complement(5541..6860)
                     /gene="der"
                     /locus_tag="MJF80_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBZ0279790.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(6894..8222)
                     /locus_tag="MJF80_001119"
     CDS             complement(6894..8222)
                     /locus_tag="MJF80_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBZ0279791.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEVGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     DALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(8460..8660)
                     /locus_tag="MJF80_001120"
     CDS             complement(8460..8660)
                     /locus_tag="MJF80_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279792.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(8662..9594)
                     /locus_tag="MJF80_001121"
     CDS             complement(8662..9594)
                     /locus_tag="MJF80_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBZ0279793.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(9639..10571)
                     /locus_tag="MJF80_001122"
     CDS             complement(9639..10571)
                     /locus_tag="MJF80_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBZ0279794.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(10626..11915)
                     /locus_tag="MJF80_001123"
     CDS             complement(10626..11915)
                     /locus_tag="MJF80_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0279795.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(11944..12636)
                     /locus_tag="MJF80_001124"
     CDS             complement(11944..12636)
                     /locus_tag="MJF80_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0279796.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(12651..13412)
                     /gene="pgeF"
                     /locus_tag="MJF80_001125"
     CDS             complement(12651..13412)
                     /gene="pgeF"
                     /locus_tag="MJF80_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBZ0279797.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(13409..13870)
                     /gene="nrdR"
                     /locus_tag="MJF80_001126"
     CDS             complement(13409..13870)
                     /gene="nrdR"
                     /locus_tag="MJF80_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBZ0279798.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(13892..14155)
                     /locus_tag="MJF80_001127"
     CDS             complement(13892..14155)
                     /locus_tag="MJF80_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBZ0279799.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(14259..15032)
                     /gene="sigG"
                     /locus_tag="MJF80_001128"
     CDS             complement(14259..15032)
                     /gene="sigG"
                     /locus_tag="MJF80_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBZ0279800.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(15124..15846)
                     /gene="sigE"
                     /locus_tag="MJF80_001129"
     CDS             complement(15124..15846)
                     /gene="sigE"
                     /locus_tag="MJF80_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBZ0279801.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(15871..16743)
                     /locus_tag="MJF80_001130"
     CDS             complement(15871..16743)
                     /locus_tag="MJF80_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBZ0279802.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(17033..18307)
                     /locus_tag="MJF80_001131"
     CDS             complement(17033..18307)
                     /locus_tag="MJF80_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBZ0279803.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(18591..19748)
                     /gene="ftsZ"
                     /locus_tag="MJF80_001132"
     CDS             complement(18591..19748)
                     /gene="ftsZ"
                     /locus_tag="MJF80_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBZ0279804.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(19938..20291)
                     /locus_tag="MJF80_001133"
     CDS             complement(19938..20291)
                     /locus_tag="MJF80_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBZ0279805.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(20390..21064)
                     /locus_tag="MJF80_001134"
     CDS             complement(20390..21064)
                     /locus_tag="MJF80_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBZ0279806.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(21069..21767)
                     /locus_tag="MJF80_001135"
     CDS             complement(21069..21767)
                     /locus_tag="MJF80_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBZ0279807.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKAIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(21790..22530)
                     /locus_tag="MJF80_001136"
     CDS             complement(21790..22530)
                     /locus_tag="MJF80_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBZ0279808.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(22800..24026)
                     /gene="murG"
                     /locus_tag="MJF80_001137"
     CDS             complement(22800..24026)
                     /gene="murG"
                     /locus_tag="MJF80_001137"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBZ0279809.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(24044..25174)
                     /gene="spoVE"
                     /locus_tag="MJF80_001138"
     CDS             complement(24044..25174)
                     /gene="spoVE"
                     /locus_tag="MJF80_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBZ0279810.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(25226..26581)
                     /locus_tag="MJF80_001139"
     CDS             complement(25226..26581)
                     /locus_tag="MJF80_001139"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBZ0279811.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(26600..27568)
                     /locus_tag="MJF80_001140"
     CDS             complement(26600..27568)
                     /locus_tag="MJF80_001140"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBZ0279812.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(27609..28982)
                     /locus_tag="MJF80_001141"
     CDS             complement(27609..28982)
                     /locus_tag="MJF80_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBZ0279813.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(29149..31128)
                     /locus_tag="MJF80_001142"
     CDS             complement(29149..31128)
                     /locus_tag="MJF80_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBZ0279814.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(31140..31487)
                     /locus_tag="MJF80_001143"
     CDS             complement(31140..31487)
                     /locus_tag="MJF80_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279815.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(31968..32903)
                     /gene="rsmH"
                     /locus_tag="MJF80_001144"
     CDS             complement(31968..32903)
                     /gene="rsmH"
                     /locus_tag="MJF80_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBZ0279816.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(32933..33679)
                     /locus_tag="MJF80_001145"
     CDS             complement(32933..33679)
                     /locus_tag="MJF80_001145"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBZ0279817.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVVGGIIAHVYLSKKNKNNISEE"
     gene            complement(34073..35359)
                     /locus_tag="MJF80_001146"
     CDS             complement(34073..35359)
                     /locus_tag="MJF80_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBZ0279818.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LEYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(35374..36639)
                     /locus_tag="MJF80_001147"
     CDS             complement(35374..36639)
                     /locus_tag="MJF80_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBZ0279819.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            36955..38052
                     /gene="ychF"
                     /locus_tag="MJF80_001148"
     CDS             36955..38052
                     /gene="ychF"
                     /locus_tag="MJF80_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBZ0279820.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        11776 a         7119 c         3897 g        15465 t
ORIGIN      
        1 acaaaataaa aaaataatat ctttttttag attatattct ttagattcta atttataatt
       61 attatttatt attccatcat aatcacttat atttgactta tttttatttg tatcttgatt
      121 tttgttagaa atactttccc tataaggaaa agaatgttca ttggtaccaa atactgattt
      181 tgagcttaaa tcataattat catcattttc tgtaattgaa tttttattgt aaaatccttt
      241 ttctttatca gaattatttt ctttattacc gtctttaggt atatcatgag gatttttatt
      301 ttcattgcta tcagtacttg attctacatc atctttagga gtatttggtt gtttctcatc
      361 agaattatca ttttcattat tattattttc attattattg ttttgattgt tattactatc
      421 attattttca tttccattat tcccatcttg attgttatca tcctcatctc tatccgggtc
      481 gtctctacct cctcctccac ggtcaccatc aatatcatca ttttcccgtt catcatctgg
      541 cgtctcttgg ttgtctccac ctcccattat aaatcctggt ttaaaaacta tatttgaata
      601 cccttctttt aatcctccta caacttttac tctaaaacaa cgcaacttaa aatcttcaaa
      661 aaaaagtata gcttgatctc taatatcctc ctgctcttca gatatccaat tgattccatc
      721 gcttgaatat tcaacaaaaa ccttacttgc actatacggc atatcataaa ataaaacagc
      781 tacatatcca ttttttgtat tttgaaattc cagttccaaa ttgtctattg gttttttctc
      841 tataacagat acattcaaaa taggaactac atcatcattt attataattt tttctttatt
      901 taacatttct gttttaaact ttcctgttat attaaaatct tctccctttt ccaacttttc
      961 ataataatca gtaaaatccc attctacatc tatatcaagt cgattaccac tagatgtttc
     1021 taataccata tatttgggaa attttatatt ttctatttta tcatctttac ttacttttat
     1081 ttcatcacta aatacaccaa ttcccctatg acaacatttc acaatattat agccagaatc
     1141 ttcagctata gagggtataa gctcattata atcatcaatt atataaactt tcttactttc
     1201 tgatataatt gattgtgcca acttattatt atctcttagt aacccacttt cattgttgct
     1261 actatatgct tttattgaag tttcttctat ctcaacatca cctttagaat aaacacctat
     1321 agcatgttct ccattcactt caatatcctt atttagtatt ctaatatcat tttcagtata
     1381 aattcctatt gcatcttttc catttgcctt tattttttca acagattcgt aggatgattt
     1441 aatatgtaag tctcctcctt cttcaacgta taaggctgtc ccatcagtag caattatgtt
     1501 gtttatacca atacttatac ttccaccatt ttctatatgt attactcctt cttttcctcc
     1561 ttcacctatt atattcaatt cattatcatc aataataaat ctcccatgat tttttatcaa
     1621 tattttatat ttgttggtat ctattgtaaa attactgtca taaggaatat aaaatctaaa
     1681 ctcttcatct attaccatat cacaagaaag agtataaact atttcttctt tattattttt
     1741 attttcttga acccaccttt caaaatcttc aagacaattt tcctcttgtc caaaaacttt
     1801 atgtggaaaa cttataaaac acaaaactat tatactcata aaaataaata tctcatactt
     1861 tttcatctta cactcctaga aatttttagc tttgaaacaa tcctgtcgaa acatatactt
     1921 cacccactat aatttttcca caattatcaa ttattgtcaa tttatataag tatctttatt
     1981 aattgttata aataaaaagc tacttactaa agcaaacaga cgtttcactt taataagtag
     2041 ctatattttt ataacaaaat agttataata ttagaacttc cttcatttac aattttttgt
     2101 aaagtctttt gcattttaac tcttatctct tctggcatag tatataattt actttgtaat
     2161 tgctctttta ctaagtcatg tagtgattta ccaaacatat ttgattccca taaatctgct
     2221 ggttgctctt caaatacttc cataagatat tttatcatct cttcaccttg attttgattt
     2281 ccaactattg gagaaacctc tgtagatata tctgctttta taatatgaag tgatggtgca
     2341 ttggctttta acttgatacc atattgtttt ccttgtttca ttatttctgg ttcttccaga
     2401 ctaagttctt ctaaggaagg agctactact ccatatcctt ttatcttggc atctattaaa
     2461 gcactttcta ttttatcata ctcattttta actttagata gtctagttat cagactaagc
     2521 agttgataat ctccttcaat tttaaatcca cttttttctt caaggacatt atagaataaa
     2581 tcttgctttg tctgtaaatc aatatttata acaccttctc ctagctctac attatcaaca
     2641 cctgtatctt ccaaaaactc taactcagaa aatccttgta taataccttc tatatccctt
     2701 attttaccta tatctataat actttgtttt aatgttgtta ttatgctact ttttatccaa
     2761 tgatttcttt caagaccttc aacccatttt ggtaagttga ttcttatttc tgtaagtgga
     2821 aaatcatata atacagcttc cataacctcc tctatatctt cttcttccat ttcaaccaca
     2881 ttcattggaa gaactggaac ttcatacttt tcttccaatt cacttctaag catactcgtt
     2941 tcctcacttt ttggacttaa cgtatttaaa accactgcaa atggtttttt aagatttttc
     3001 aattcttgta taactctctc ttctggttct acatagcttt ttctgtcaat tccagtaact
     3061 gaaccatctg tcaagacaac tatccctatt gtagaatggt cttttataac ttttttagtt
     3121 cctatctctg ctgccttttc aaatgtcata gcttcttgtg accaaggagt ggatactaat
     3181 ctttgttttc caccttcctc atgccctaaa gctccttcaa ctatgtatcc aacacaatct
     3241 accattctta cttttaaaga tactgtgtct tttattttta tttctacacc atctgctggt
     3301 acaaattttg gttctactgt cattatcgtt ttacctgaac cactttgggg tatctcatct
     3361 cttgttctat cctttttaaa ttcattgtct atattaggta taaccaactt ttccataaat
     3421 tttcttataa aagttgattt tcctgttctt acaggtccaa ctactccaat atatatatcc
     3481 ccctgagtcc ttttggatat atcttcgtat atgttattat tcatattatt cctcctatac
     3541 atctaatctt attaaattat atttagagtt aagggttaat attcttaaaa tagaaaaata
     3601 aagactagca atattaattt cccctatata caaaaactag taaactacaa tgctaattga
     3661 aattattaaa atccattaca ttaaaagaca taattatagt catgataatt acaaattata
     3721 tataaaataa tttattatat cgtatataca tagactatgc taagtaaaat taaaatagcc
     3781 ctctttatat taaagagggc taaaacaaat ctattatttt tttattttat tatacaatag
     3841 tttatatatc atcacctaaa actacttctt ccatttcatg ctttttactt ctcatcatta
     3901 gttctattcc aacttcttta ggatttgaac ctttctttat tacagaatat atagcattag
     3961 ttataggcat atctatatct aatttttcag caacatcatg tgctacttca gtagcagtta
     4021 tgccttctac aaccatcttt acttctttta aagtatcttc taaactcatc cctttaccta
     4081 tgagtatacc agctcttcta tttctactat gcatacttgt acaagtaact attaaatctc
     4141 caatacctga aagacctgca aaggtagaca tattagctcc catagcaatt ccaagtctgc
     4201 tcatttcact aatacctctt gtcataaggg cagcttttgc attatcacca taccctaatc
     4261 cgtcacatat cccagctcca aatgctataa tattttttaa tgctcctcca agttctactc
     4321 caactatgtc tggattcgta taaactctaa gtttagggct cataaataaa tcttgtatca
     4381 tctgtgctat ttttaaatct ttagaagcaa ctactacggt agtaggaata tcccttgcta
     4441 cttcttcagc atgagatggc ccagatagta ttacatatgg attttgtggt aattcttctt
     4501 cacagacttg tgataatcta agcccagtcc ctttttcaag acctttagct acattaacaa
     4561 taacttgtcc ttcttttata aatggtttta ttttttggca agtacttctt acagcctgtg
     4621 atggaactgc taaaactatt accttacaat cttttatagt tttttctata tcagtactta
     4681 tagttatatt atttggaaat aatactcctg gtaaatagtc tgtattttct ttagttctgt
     4741 ttattttttt agcttgttcc tcttttcgag tccacatgct cacatcatta cctttttttg
     4801 caagccctaa agctaatgca cttccccagc ttccagtacc taatacacat actttctcca
     4861 tgtttaacac cacctgagta taatatattt actattcttt cttttgacca atttttctct
     4921 ctgtcccatt taataatcgc tttatatttg ctctgtgatt atatattact gatgctgtta
     4981 agaacagagc tactattaaa ccagctttat tttttaccat aatcataaat attggtgaac
     5041 atcctattcc tacaacagaa cccaatgaaa catactttgt tatagctacc accaatataa
     5101 atactgctag acacattagt gttattactg gatttacagc tagcattgcc cctagtgaag
     5161 ttgcaacacc tttgcctcct ctaaatccta aaaccgctgg ccaattatga cctgctacaa
     5221 cacatataac tgccagatat ccagctaaaa gagtatctat tccaaccaat cttgctgcaa
     5281 attctgatat taaaacagcc attacacctt taagaacatc acctaaaaat gtcattgctc
     5341 ctgctctttt tccaagtgtc ctaagtacat ttgtcgaacc tgcattacca gaaccttgag
     5401 tccttatatc aaccccagca attcgctttg caactatgta tgaggtagat atatttccta
     5461 aaagatatgc aactacagca ataattatat aactaaatat ctccactgaa tttcccccct
     5521 ttagttatat tttaagatat ttatttttta tttttttgtc tatactcaaa ctttatggaa
     5581 gttccttcaa atccaaaatt ttctcttatc ttattctcaa ggtatctttg atatgagaag
     5641 tgagttaaat ccttatcatt tataaacaat gtaattttag gaggtctaat gtcagtttga
     5701 gtcccatagt aaatttttaa tcttctacct ttgtcagatg gtggttgatt taacattaca
     5761 gcttctccaa ttacttcatt cagtgctgaa gttgatattc tcttagaatg ttcatttgac
     5821 acatattcaa ctgtatctag tatcttgttc attctttgat tagtttttgc agaaacaaat
     5881 acgattggag catacatcat aaatggaaat ttttctttta tatcttttgt gtaattactc
     5941 atagtcttat tatctttctc tattaaatcc catttattga ttacaaatat gcatcctttt
     6001 ccttcatcat gtgctatacc tgcaaccttt gtatcttgtt ctgttacacc ttctaaggca
     6061 tctattacta tcaatactac atctgctcta tctacagcac tcatagctct tattacactg
     6121 tatttttcta ttgtctcata tattttactt tttcttctaa gccctgctgt atctattaga
     6181 agaaatttct gtccattttt ttcaaaataa gtatcaattg cgtctctagt agttcctgca
     6241 attggactta caattactct ttcttctcca agtatcttat tgagtataga actttttcct
     6301 gcatttggtt tacctgttat agctactctt attatatctt cttcatactc tgtatctaat
     6361 cctgctggaa aattttcaac tatctcatct aataaatctc caaatcccat actattggct
     6421 cctgaaactg caaaaggcgt tccaaatccc aactcataaa aatcgtatat attatcaaat
     6481 tgactttgac tatcaatctt attaaccact aagattactg gtttttttgt ctttctaagc
     6541 aattgtgcaa tttctttatc agcagctgtt attccagctt taccatcaac tataaaaagt
     6601 attacatgtg acatgtccat tgcaagcatg gcttgatttc tcatttgaga taatattata
     6661 tcttcgctat caggttcaat tcctcctgta tccactaatg taaaatattt atctaaccat
     6721 tctacttcag caaatattct atctcttgta actccaggtg tattttcaac tattgatatc
     6781 ctttttcctg caaatttatt aaatattgta gacttaccaa cgtttggtct cccaactaca
     6841 gctacaactg gcctacttat actcatttta tttctccttc catcttttgt tttctatttt
     6901 aaaattttat ccacaaaatc ttttccttca ttcaaacaag ggataatttt tattctcata
     6961 agttcttcta attgatttaa ttcaatatta tctaagaaaa tttcttcatc tgcttttagc
     7021 atacttcttg ttatataaag agcgtctcct aattctttat cttcaagttg ttcttttaaa
     7081 tccttagctg ttaaaagacc tgatacagtt attgtctctc caaaaaactt gtttttgatt
     7141 tcatatacat ttacttgaag atttttaaac ttgtccatca tagcatctgc catactctgt
     7201 ataaattcat atgcagaatg accagtagct attgatactt tcttctctct ttttagaatc
     7261 ttctcaggca atatatttag atactcttca atctctgttt ttaacttacg tatcatacct
     7321 acaccatctt caaactgtaa aaaaccttca tattcttcat atccaggcaa ttcactatta
     7381 gatagtatat aaaattcatc tgataaaaat gcaaatcgag tacctaattt ttcaagatat
     7441 ttctgttgta tttgatgtat ttgctctata gttttacttg cgctcttatc attaaatata
     7501 tttaattcta ctagattttc tctatgcttt gtaatcccaa ctggaactat cgcaacactg
     7561 tttacatacg gataaagttg agctagttct tttattgttc tatccaattc ctttccatca
     7621 tttatacctg gacataaaac aatttgacag ttcatttcta tatgagcatc tgccaatctc
     7681 ttcattatac catataattt ccctgcaaat ttattgctta tcattttttg tcttaactct
     7741 ggattagttg tatgcacaga tatatttata ggacttattc tatattttat aatattattt
     7801 atatcttctt cactcatatt tgtaagtgtg acaaaatttc cttgtaaaaa agataatctc
     7861 gaatcatcat ctttaaaata aagagtttct ctcatacctt ctggcaattg gtctataaaa
     7921 caaaacatac acttatttct acagctttta gcttggtcaa ttatcggatt tgtaaattca
     7981 acacccaaat cttcatcata gtctttttct atctcgtata tgtagacttc gccatctttt
     8041 ttttgtattt ctacatctaa atattcatca cttagtaaga atctatactc tattatatca
     8101 tgtattggct gttcattcac agatataagt aaatctccta cctctatacc cacttcttct
     8161 gcaatactat ctttataaac cttacttata atattattta ctttttttac atttacttcc
     8221 aatattttca ccactctttt gtattcatca atgcttatta taatgtattt taatatgcaa
     8281 tttatttatt tattatattt tatgatagca tttttaagca tttcatgtca taataacaca
     8341 ttttaattct aatagtcaat ataagtaaat agctataaaa tagattagaa caaataatat
     8401 tcaaatttaa gtttcttaaa aatttaaata ttatttgttc ttaaaaaaat tttataaatt
     8461 taatgtttta ctatgattaa cgtcccattt gtcatatcat gaaccattcc acctacacac
     8521 tttgacatca acaatgcctt attttgtaag tctttctcat catttgcata aaatattgga
     8581 caacctccac catttttcat attcttatca agtgtaacta tagctagggt atattcattt
     8641 aaacctatat ccattcccat cttatgcact tcctttcttt cctattttat aattttttag
     8701 tgataagttt tttcctttag agcttgaaag tattgggcat gactttacag cttccattac
     8761 tttttccaca ttcttatcta ctggtaaata agcaatcatt atactttcat cctctttatt
     8821 tcttctaggt aatggtacga atgaaggttc tccataatct ccatataaac ctaaccttga
     8881 atatatattg tatactattg cttgtctttg accggagtca aataatattc ctgcattagc
     8941 ataatcctta tttttaggaa ttatttgtat tcctacacct ctattaatat atacttctct
     9001 atcgcttttt agacctatat ttgttatacc ttttaaatct cccaccttta gtatagattc
     9061 gtccacaaaa ctaatttcag caggcattac atcagctatg tctccaatac tttttctagt
     9121 aagaagtttt tttaatataa aggctaacac taatccacaa attgaactta ttaccaaact
     9181 catcccttca cttattttcg tttctgcaag tattacataa tacaatccta ttgttaaaaa
     9241 agatgtaact atacacatat aatttcttac ttcataggtt ctagctatct cttctatgaa
     9301 tgcagctcct ctctttacaa gttgaacttc ctctaaattt tgcaatgtat ttcttctatt
     9361 tgctctcact tgtctaaatt gttctgctgc tagagataaa aatgttatgg atgtataaga
     9421 acgatttata agagctggaa ccaataatgc tcctaatgaa gaagcgacga atgctaatat
     9481 aagttgagat agtaaaacat ttggttgcgt tggatactgt ttttggtcta aattcaacat
     9541 tataattcta gatgttatac caataataac agcaacaaaa aatactctat ccattaaatc
     9601 actcctaaaa tatttaattt gaaattttac atgtaaatct acaaagtctt tctagaaaaa
     9661 tcactctgct ttccttttga tgtttctatg ataggtgtac ttttcactac atctatcatg
     9721 acatctatat ctttgagtat tggcatatat ggtataacca cagtactttt ttctaaattg
     9781 gtcttagata tagcaacaat atcaacctca tctacatctc tatctatccc catatgaata
     9841 aatagattat gtattatagc ttgtctttgc cctatatcat ttactattcc aaatgcacct
     9901 aaatctcttg gtataacttc tatagccaga cattcatttc tatacttttc tcttgtactt
     9961 tctagaccta tgttagttat tattacacca tttaccatta aaataggccc ttcaaaactt
    10021 atttttgcag gaactatatc tgctatatct gctatactgt tccttcttaa aaatcttcta
    10081 aaaatcaaac caacaatagc tcctgacaca atagctaaga ttgtgcaata aaaaaagcta
    10141 aggccatact ttcttgcaaa aacgatataa actatcgaag ataccagtgc agaaaaaaga
    10201 cttatataac ttctagattc atacgtagat gctatttctt caacgtaagc atctcctttt
    10261 ggtactaatt cttcattatc gatattcttt agtgttattc tttcttgttc tgcaagacct
    10321 tgaaactgtt gtattgctac tgcaaaaaat gtcaaggctg caaactcctt atctataagt
    10381 gcaggtaatg ctatagcacc taacgaagct gaaagcccag atataattat ctgttctaaa
    10441 taatcttgtg gtcttgatgg gtattgtttg tctgtaactc ttaaaactaa tcctctacat
    10501 aatattccta caactaatgc aactataaaa gagtgtctaa ataaatttgc attcaataaa
    10561 ctttcttcca ttttctcagc tccttctctt atatagtttg tgcaaaaaac aaaaaaaatt
    10621 cccttctatt taaggaaatt tttttgtaat attgtaacta taaattcgct accaatgcca
    10681 agttcacttt ttacttttat acatccattt agtgatttga ctatatgctt tgttatagcc
    10741 aatcctaaac ccgttcctcc tacatcccta cttcttgctt tatctaccct ataaaatcgt
    10801 tcaaatatgc gttctacatc ttcttttggt atccctacac cattgtctat aactttaatt
    10861 acgatttttt cttcatcttt taacactaca acttgtacaa tcccattatc ttgggtatac
    10921 tttatagcat tatcaactag gtttaagaac acttgcttta tatagtcttt attggatagt
    10981 atacttattg atttatcatc acatttatat aaaacttcta tatttttttc actagctgaa
    11041 tgttgagtta tttcatatac ctctttaaat acatcatata ctacaacatt ttccataaga
    11101 agagtatctt tattttctac aaatgataac agcaatatat catcaatcaa cctttttaat
    11161 ctatctgatt cgctttctat tatttctaag aatctatttc tagtattcac atctatattt
    11221 tcatttgact ttagtgtttc aacaaaccca cttatagatg taagtggtgt tttaagttca
    11281 tgacttacat ttgcaacaaa atcacttctc atattttcaa gtttaacttt tttagtaata
    11341 tcttctatat ttactatcga acctattatt acgttgttag tattctgaag ataaactggg
    11401 tctactttta tcttatatac gatatcatct ttagtagtaa tattagcagt ttcattatct
    11461 ttagaaccaa taaattgaag tatttgtttt aatatttctt cttcttttat tacaacatta
    11521 atattatgac cttcaactaa ttgatttggt tcactcttta tcatttcttt tgcttcatca
    11581 tttatcagaa gtatatttcc atttacatct atagctaaaa ttccatgaga tatactcttt
    11641 aatatagacc ttaactgtaa atgtttatac tgtacttctt ctaaggtttt atccatattc
    11701 tttatcatat gattaaaatt tctagctaat tcgccaagtt ctcctttggc tgatatgtta
    11761 agcctagcat gatattcatt attacttact ttttttgaga cgtatataaa ctcttttaaa
    11821 tattttctta atgttattgt gtatcttaca gctaatataa caatggctat ggacaacata
    11881 acaataaaaa aatatatact atttaaatct tccacaagat aacactccta aaatataacc
    11941 tttttaatct attttgtatc ctacacctct tatagtctct atatattttt ccgatttatc
    12001 ttcatcttct attttttttc ttaaatatct tatatgaaca tctactgttc tagtttctcc
    12061 atagtattca tacccccata ttttatccaa aagataattt cttgaaagta cttttccttt
    12121 attttgaatt aataatttta acaattcaaa ttccttaaga gttaactcta tcttttcaga
    12181 gcctttggta acttcatgtt ttgataaatc tagttttaaa ttacctgtag taagtatatt
    12241 gttttcttca cctaagctag tcaaattata tctccttaaa acagaactaa tcctagctaa
    12301 caactctttt atactaaatg gcttagttat atagtcatca gcacctattt caagcccttc
    12361 aaccttgtca ttttccatat ttttagcagt aagcattata acaggaatat tttttaagtc
    12421 cttatcactt cttatttttc tcaaaacttc tattccactt atatttggta acatccaatc
    12481 taaaagtatt aaatctggtt taatttcctt agcctttata aatccgtcaa atccatcata
    12541 tgaataacta acttcgtaat ttgacacctc caaattgaat tttaatagct caactatatg
    12601 catttcatcg tctataacaa gcactttagt attcattaaa aagctcctct ctattttatc
    12661 tgtagtaccg ttcctattcg ttctgatgcc ttattatgtt ttctataaga gtggaattta
    12721 tctgctctac aactagtgca taaatttaaa tttattatat tttctttttt aactccacta
    12781 catctaagca tatactcatt aattttccat aaatctaaat agtaacttcc ctcttttatt
    12841 atatagaact tttcatcatt atttgtaaga attgtgttaa atttttctac taaatcttca
    12901 gacacttcat aacaacatgg tccaattgat ggtccaatta tacatactaa atcttcagga
    12961 actgttttat agcattcaga cattaatttt attgttttat gacctatatt actaaaagtc
    13021 cctctccaac ctgcatgagc cagtcctatg gctttatttt tagcatctat aattgatata
    13081 ggtacacagt ctgctgtaaa cagtaaaagt ggtacttttt ccaagtttgt tataagtgca
    13141 tctccatctc ttcttttccc tatagtatct ttattgacta tattaactat atctgaatgt
    13201 atttgagaat tactagttag attttcaaat gtcaaatcac attctttaca tacttttaac
    13261 atatcttctc tattttttgc atctatattc ttacatgtta taactagatt tacagaattt
    13321 agtttttcat caagttcttc acaattacca cttaaattaa catactcttt aatatcttgt
    13381 acatttttta tatttatata atctttcatc atttgtctcc tttttctatc agtatacttt
    13441 taagttcatt tacaaaagta tttatatctt taaattgtct atacacagaa gcaaatctta
    13501 cataggcaac ctcatctaac tctttcaatt tatccattac catctcaccg atttttttgg
    13561 tttcaatttc ttctataaaa catttattta tttcattttc aatatgtaca acaatacttt
    13621 caatctcgtc aatagaaaca ggtctttttt cacatgcttt taacaatcca tattttatct
    13681 tttctctatc aaaatattct ctactattat cctttttaat gaccattaaa ggagttgttt
    13741 ctattttctc atacgtagta aatctctttt tacatgattc acactcacgt cttcttctga
    13801 tagattttaa atctgtatgt ctagaatcta ttacctttga ttctttataa ttacaataag
    13861 gacattgcat ctatattcct cctttgttta actatatctc aaactcatct aagcttaatt
    13921 ctgaacctat atttactatt attgtatcac aacctatttt aagtatatca ttccaaaata
    13981 taacttcttc atcccctcct cttgaaaaaa aacttctact tccatctcca aaaatggtaa
    14041 atgagactac cttaccttcg tgaatatcca tgtctatgtc tattataaac cccatccttt
    14101 ttccattttt tacatttata atttcttttt ccattatatc agaaactcta atcatattgt
    14161 actcaccctt tctttaaaaa taaatatgta tcgaagtaaa aaaaaatgac ctatataggt
    14221 catttaaatt aaaataatgt ttacagtcca tggattactt atacatattt tctcatattc
    14281 tttaaagcat ttttttcaat tcttgatact tgtgcttgag atataccgat ttcatcagca
    14341 acttctatct gagttcttcc tttgtaaaac cttaaatcta ataccaactt ttctctacta
    14401 tttaatttct taatagcttc tttaagtgat atttcttgca accaattttc atcagtatct
    14461 tttttatctt gaacctgatc cataacgaat attgcatctc cattgtcttg atatactggg
    14521 tcaaacagag atattgggtc ttgtatggca tcaagtgcca ttacaactga ttctacttct
    14581 aattctaatt cctttgctat ttctgatact gtaggttctt tagaatttgt tctaattaat
    14641 ctttctctaa cttgtagtgc cttatatgct atatctttta atgacctact tactctaatt
    14701 gggttattgt ctcttaggta tcttctaatt tctcctataa tcataggaac agcataagtt
    14761 gaaaacctta cattttggct taaatcaaag ttatctatag cttttataag accaatacaa
    14821 cctatttgaa ataaatcgtc tatattttct cctctgttgt taaacttttt tattacactc
    14881 aagactaatc ttaaattccc tcttacaaac tgttgtctgg cttcttcatc accatttttt
    14941 atttgtaata aaagttcctt catttgctta tttttaagaa ctggaagttc tgatgtatta
    15001 acaccacata tttcaacctt attaacttgc atatattttc agtcctctct caaaagtatt
    15061 catctattga aattatttac atttccaatt tttttatact ttttaagaag aattaattta
    15121 gcattataca aattttttca tttctttttg caaccttgag attattttct tttcaagtct
    15181 agatatgtag gactgtgata ttccaagcat tccagcaact tctttttgtg ttttttcaat
    15241 acctttatca ataagaccaa atcttaattc cataatttgc ttttctctgt cacttaatct
    15301 gtcaagtgcc ataaccaaca aatctctatc tatttcttct tcaattattt tgtatatttc
    15361 atcattttct gtacccaata catcagataa taaaagttcg tttccatcta aatctatatt
    15421 taaaggttca tcaaatgata cttctgtttt ctttttatta ttttttctta agtacattaa
    15481 tatttcattt tctatacatc tcgaagcata agttgccaat tttatatttt tattcagttt
    15541 aaaagtatta actgctttta tcaaacctat agttcctatt gaaatcaaat cttctacatc
    15601 tatacctgta ttttcaaatt ttctcgatat gtagactact aatctaagat ttctttctat
    15661 taaaattgac tttacacttt catctgtttc tagcttttgt aaaagctcca tttcttcttc
    15721 tggtttcaat ggaggaggaa gtatatttgc ccctcccata tagtatattc cctttggctt
    15781 tattattttt aatttaatac ctaacatagt aataaagctt attattcttt ctttcaatcg
    15841 taacattcct tgtccccctt aaattttcac ctaaaatcca ggcaatatat ttggattcat
    15901 tattgctcca tattctctat cattaaaatt tgaaatacca agtattatat ttcctatctt
    15961 ttgctcatct acttctatat agtcagcttt taagcctatt attatgcttg tcttattact
    16021 tccagcatgt ttataaggta ttattctaac tctacctgaa gttttatcat ctaaacttcc
    16081 tataatttct tgtgctttct tcatatctat atcttcataa ttaatattta atagtttttc
    16141 tggaataaat ccttctaaca cactagactt aactattatt acatcacttt gacttattgg
    16201 gtcttttaac aaattaccac tatctactaa agctttacag caaaaacttt tttctagtaa
    16261 gtttatattt atagtctttg taagctcctt aatgtactta agcattttca aatctttata
    16321 aatgtatttt aataattcac aactcacata agtacatgca attaagaaag atatttttaa
    16381 gtgacttatt cctgtaaaat aaattataaa ataagtgctt ccagaaataa aaatgtttac
    16441 tagataaaaa actaacgcta catgaacata atcttttttg ctcttacttc taaatgaaat
    16501 taaagttatt atactcatga ttattatttt aaatggtaca gtaaatagta tattcattga
    16561 tggatacaaa taagcaacag aataggcacc tcctaaacta gcccccaata acttattttt
    16621 tttaggatta tatcttttag taagaataga agtacaactt ataataatat aatttattac
    16681 taggttttct ataacatagt actcaatata caccatgtat ccccccttaa aacctttaaa
    16741 catattataa gatagtatgt acaaaatttt atgtcacttt tgatggtcga atccaaaaca
    16801 aattctattt aaaaagaaca atgatatatg tcttatactt tatgtcgtca aaagatattt
    16861 ttttaatgcc aaacaaatag acttttttgt agaatccaat atatctaggt agaagttatt
    16921 cgctctactt ttggtatatt tacaaaaaaa gactacataa tttaataata tatcaagtaa
    16981 atcttttact tatataaaat caaattatat agtctgtaaa ttcaaatata aattattgtg
    17041 ctagtaatcc atttgcatat tttgcagatt catttagtgc ttgttctgga gttatgtctc
    17101 cagctatcat catttgaagt tgtttccata aatattcata tatctctact ccatgaggtc
    17161 caactgtcaa tggtctatac atatgtttat ctcttgtcac catttcttca aatcttgggt
    17221 ctccttggta tttttcacct ttagcaactg gagctctagg tatttctttg tggaaagctt
    17281 ccattgattc tttgcttagc atatactcaa gaagtttaaa tccaagttct ttatttttac
    17341 atgctgacat taatgttaaa tcatctactg aagcaaaact tccaactgtc ttatcttcta
    17401 atgatgttac atatccccag tttaaatccg ggaatgattt atcaaaagta cttgtagcag
    17461 cagaggatag cattatagta aatgcagctt ttcctggtcc aaatacactt tcaatcattt
    17521 cattactatc ttttgataaa gcatcttctg gaatatactt tttcatatca tttataaagt
    17581 ttgcagcttt caagccttct gcattattaa atttaactga ttttaaatca tcattatata
    17641 tatctccacc agcttgccat aaatatggat accagttcca gtttaagctt ccaaatactt
    17701 tagaacccca accttgtgca agaccccact ggtcgatttt tccatctcca tcagtatctt
    17761 ttgttgcttt ttcacagatt cttttaaaat catcccatgt ttttggaggt ttttctccta
    17821 atttttctag tatatcttta ttgtagtaca atacgcctgg attagcagct tctataggta
    17881 aaccataaat accacccatc atttttgcat catctaagta tagataatta tctgaagttc
    17941 ctgatttttc taaataaggt gttaaatctt ctaccgcacc catttctata aattgaggaa
    18001 acatctctgc atacatgtat cctatatctg gaccttcacc tgcacttatt gctgttgcat
    18061 atttttctgc gtagttatcc catggtacta tctgaaattc aatttcacag ttattttctt
    18121 tttcaaattt ttcaaatatc ggcttccatg catctgcatc atggtctgtt aatggtgcag
    18181 accatacaac taatttttct ttaccctctt tgcctttaga atcttctttt tttgaacatc
    18241 ctgcaagaat tgatactgat agaatcccta ccataactaa agaaagaacc ttttttagtt
    18301 taatcatgct taatcccctt tcagacttta atttgtcttt ttcctcaaat gtttacattt
    18361 aattcagatt taatattaac aaataagatt actcttgtct aatcataaaa aactatacta
    18421 actcctataa ctatattttt tttctatacc tattagattt aattcatact aaaaacttac
    18481 tttttaaatt ttatcaatat ttaaaaattt ttttaagtct agccacaaac taactatcta
    18541 aattgagcaa aataaaaaga agtctattga cttctttata agcttacata ttatcttctt
    18601 cttcttagga atgtaggtat ctccatgctc tcatcatcat cttcttcaat tatacttgcc
    18661 tttttaggag gctctatttt ttcctcagca attacttctt cttcaatttc ttttacagct
    18721 tgttttactg ttgtatttaa tgaagaacgt atgcttgatt taggtttagt atctaaatct
    18781 aaggcaccat tttgtaatcc ttcaaatcct gtcgcgatta ctgtaatcat tatttcatca
    18841 cctaaatctt cttttataga agctccaaat ataacatttg cttctgggtc acaggattcc
    18901 ataactaaag ttgatgcctc attaacttca aataatccta agttaggacc acctgtaaca
    18961 ttaagaagta ctcctttagc tccttgtatt gatgtttcta gaagtggaga ttgaatagct
    19021 tgtcttgcag catcaattgc tctagtttca ccactagcac tacctatacc catatgagca
    19081 agtcccttgt ctttcattat agtagtaaca tctgcaaagt ccaagtttat taacccttca
    19141 actgctataa ggtctgaaat tgattgtata ccttgtttta atacatcatc tgcaactgca
    19201 aaagcatcta acattgatgt atttttttgt actatctgta aaagtctatc atttggtatc
    19261 gttataagag tatctacttt tgatttaagt tctgctattc caccttcagc gttcttcatt
    19321 cttatcttac cttcaaatgc aaaaggtttg gtaactacac ctactgtaag tatccccatt
    19381 tcttttgcca agcctgcaac cactggagca gctcctgtac cagtaccacc gcccattccg
    19441 gctgttacaa agaccatatc agctccttga agtaacttta ctatttcatc tttactctcc
    19501 tcagcagctc ttttccctac ttctggattt gctcctgctc caagtcctct agttaatttt
    19561 tctcctattt gaactttata ttcagctttt gatgtatata aagcttgttt atctgtattt
    19621 actgatataa attcaacgcc ttttagttga gcttctacca ttctattgac agcattgttt
    19681 ccaccgccac ctacacctat tactttaatt tgtgcacatt cttctaattc tacgtcaaag
    19741 tttagcatca taaaccccct tctctcatga ttcaaagtta tcgtatgtat taattatatc
    19801 attttcttct tttattttgt taacctttaa gtaacatatt ccacaaaaac gtaaattttc
    19861 ctttttaaat ttcttactta tattgtcaaa tattactctt taagtataaa ataatttacg
    19921 tatatattaa atgctcttta atttttacgt tttttgttca taatattact aataatatat
    19981 cgtctaatta tagataagtt attaaacaat ctcataccaa aaactaatac agcaacatag
    20041 taaagagaaa ctcctaactt ttctcctata taagtcaata atatagccaa tatagcatta
    20101 cctatgaaac cagtaataaa aattacatta tcatagttat cttcaaaatt ggattttatc
    20161 gctccaaaaa tagaatccat tattgcaaga atagctactg acatatatat ggaataatcc
    20221 atagaatatg taaatggaat atagtaacct actactacac caaatgctaa tcctcctaaa
    20281 acccataaca taaatcttca cctctttatt ttactttgaa acacaactac attacaatcc
    20341 tttttattaa ttttttttac tttttcttta tttttttgta tttttcgttt tattttgcaa
    20401 ataaattcac tttatttcca ttattttcct gaacttttat attaattcca tatatatcct
    20461 taagagattt agcatatgat tctggagagt ttatagctgc actcaataaa tctatatctc
    20521 ctatagcttt tattataaat ggttgtccat atgtagtatc atttactgta atagtagctc
    20581 ctgaacattt tatcttactt gttgctagta ctctttctcc atttatagaa attgctcttg
    20641 ctccagcctt ttttaaatca tttaaaaccc tcaaaatatc tatatcatgt ataatcaaat
    20701 cgtttggatt ctgtccgtct tttaactctc tctcactatc cctcatagta atagttatgc
    20761 ctggccctgt aacagttgaa taaccactta actctttaac ttcttcaagc tctttttgca
    20821 taagtgtttg gatagattca tttccattct tttttgcttc tttatattca ttcaaagttt
    20881 ttttgttctt tttaagagtt tcttttaaat tttttatttc tttttttgta ttttctacct
    20941 cactttcaat actttcagtt tgacctaatg ttatgtatac tctatttggg tctagtgatt
    21001 ttatataagt tgatatgaaa acgcctaata tgaatgctcc taatataact attttctttt
    21061 tcatactatt atttcccctc tgctttgttt atatcactat gaccatttat ttctatattg
    21121 tcttctttgg tcacttttac tttggcatta taatatgttt ctatttccca gacaatacca
    21181 tactttatat taagtgctga agccaatgta tcagaatcac caattgcttt tattataatt
    21241 ggttctttta tgacagtgtc attcatataa agcttgtctt cttttaaata catatatgta
    21301 ttagaaacta ttctctggtc gtttatagat atggcacttg cttgtgctga atttaattta
    21361 tttatcattg acaataataa atcataatta tatagtatac tatcattttt atcagagttt
    21421 cctaagtctt ttgtagaatt tatctcaatt tttatgcctg ttccttcaac atctgtatat
    21481 ccagctaatg tttcatattt ttctatttct gacttaagta tgtcttgttc tccttcattg
    21541 tttttgcctc tataagtctc tatagctttc ttcacactat taacttctga ttgtagattc
    21601 tctttttctt tttttaatga tttaagttct tctgtaagtt gctctcctct tttaaaagtt
    21661 gtcatccctc tattttctat attaataatt ttaagttgta aactaacaag aacacccaat
    21721 acaatacttc ctatgattat acttttattt ataaatcttt ttttcaatat atcacttcct
    21781 tacaattgtc tatccttctg gactatacac tgccaacttt ccatagttaa gatttatagt
    21841 ccctccatta gtatttttat tttgcaggtc aatcagaatt ttacttaccc tagatatatt
    21901 ataatctaag ttatcatcat tagaaagtaa gatttttata ttacttcttg taagcatttc
    21961 tatattgctt tcttttttta agtttacata atttattttt ctataaattc cttcttcttt
    22021 taaataattt aaagttttaa atacattctc cttagtttta taatttttaa attttataga
    22081 tttattatca tcaatgctat aatctacatc tactatcaag tcttttttac tttcattaga
    22141 acctcttagt tcttccaata aatttccttt tttatctata taacaataat tatcttcatc
    22201 ttttaatact gcaaatattt ctttttcttt taaacttata attatcttat tgggaagttt
    22261 tctttttatt tccacatttt ctatatatgg attttttata agtttatttt tcatatcttt
    22321 aaaattgtat gcaaaaatat tttcatttag atttacattt agttctttca ttatatttga
    22381 tttggtcacc ctcttatttc caattacatc tattttcttt acatcaaata aatcactttt
    22441 tacaattata catatacaaa aagataacat aagtataaaa actaaaacta tcatgacatt
    22501 attcgtattt aactttcttc tttttttcaa atataatcaa cccctttggt taataaatac
    22561 acctttagag cttgtttttc ctacaaaaaa attataatat ttaaagtcaa atttttgcat
    22621 tttattacat aacatccaaa taaggtatta aattttataa ataaatcaat atttcatcaa
    22681 aataaacata gcatactaca tactatttat atatttaaat ctgtatacat acaaataaac
    22741 ccctaattta taataattta gaaaatctaa tatattataa attaggggta aatatttact
    22801 tatcttttct ttattcctat taccttagct tgaccttcta tacttggagt cgtttctttt
    22861 tttatttctt ttacttcctt aataactttt tctttttttg tttttttaga agttgacttt
    22921 tgtgttgaat tgtatacttt cattatctca tcatatataa ggtctatggc ttcaggctta
    22981 cctatagttt tacttgcatt tgccatatca accaaaagtt ctctatctcc taaaagttta
    23041 aatactgctg tatttaggct ctctggagtt agattttttt ctaaaatagc aattcctgcc
    23101 ccttgttttt ctatactctt ggcattatac tcttgatgat tttcagcagt ataagcttta
    23161 ggtattatta tagatggctt tccaagagcc gttatctcag ctaatgaaat tgctcctgcg
    23221 ctacctataa ccaaatcact tgctgcaagt gcatttgcca tatcttctaa gtaaggcaca
    23281 actttttggt atggtttaag atttatatcg ctaatacttc ccataaattc atcataatag
    23341 cttttgcctg tagcaaatat aaaagctata tcttcattta ccatattctt tattacaagt
    23401 ctcattgcat catttatttt tcttgaacct ccactaccac catagcaaag taccattctt
    23461 ttttcgtcac ttattgatag attctttctt gcaatatttt ttcttgataa taaaatttct
    23521 tttctaactg gatttcctgt aaaaactaat ttatcttctg ctgcctctgg aaatctttta
    23581 tgcgaatctt caaaacttgt aagtaccttt gtaactgttt tagataaaat cttattagtt
    23641 acaccaggaa aagaattttg ctcatgtatt atggctggta ttttccccat agatgcatta
    23701 aataaaacag gtccactaac atatccacca gttcctataa ctatatctgg tttaaatttc
    23761 ttaacaatct ttcttgattg ctccaaacct ttaaaaagct taaaaactct ttttacatta
    23821 tcgaaatcta tttttcgctt aaaaccttga actgttacag tctttaattc aaatccatac
    23881 ttaggaacta tttctgactc tattcccttt tccgtcccaa caaaaattat ctcagcgtct
    23941 ggatgctcat ctcttatttt gtttgcaata gctatagctg gatacacgtg ccctccagtt
    24001 ccaccgcctg ataataaaac tttcatcaaa tcatcatctc ctgttaatta attttgacat
    24061 gctttgagat gtttaacact attcctacag ctcccataaa gatggtgagt gaagttcctc
    24121 cataacttat aaatggtagt gctacacctg tagctggcat tgatgatgtc gcaactgcta
    24181 tatttaaagc tgcttgtatt ccaatttgtg cacctatacc tataacaacc atacaagcaa
    24241 atacattaga agtcttcaaa gctattctta cacatctgta gactaaaact acaaatagca
    24301 taataactat tatacaacca atcaatccta actcttctcc tattatagca aatataaagt
    24361 cattttgtgg ctctggtata taaaaatact tttgtttact ctttcctaag cctagaccaa
    24421 acaatccacc tgaacctagt gcatatagac cttgtattac ctgatacccc ttaccaagag
    24481 ggtcttgaaa agggtctaaa aatgatgtaa cacgactaag tctatagggt tcagcaatta
    24541 ctaatgcagc aaataaagca gccccactcc ctatcatagc aaatacaatt ttcatatcca
    24601 ttccagcaac aaatatcatt acaaaagtaa ctagaattac agtacctgct gttgacaaat
    24661 ttggctgaag tattatcaac gcaaaaaata tccctggaac tacgagaagt ggtacaacac
    24721 cttttgtcaa agattttatt ttatcataat ttttttcaat taattttgca gttaatatta
    24781 ttgtagcaaa ctttgcgatt tctgcaggtt gaaatgttaa agctcctatt cccaaccatc
    24841 tttttgctcc gtttgcttct atccctaaag gggtcaaaac taacaataat aatactactg
    24901 ctatagctcc aattgctgta gcatttttct tccaaaaact ataatctatt cttgatgtaa
    24961 taatcataac tataaatcca agtacagcat atattacatt cttttttaga aaatagtacg
    25021 catcattgtg tttaaatgaa gattgtataa aacttgcact aaacaccata actattccta
    25081 caaatactaa aagcatagta gtgtaaaaca ctactccatc aaattcagtc ttcatcctta
    25141 tgtcaatttg ttttttcaaa ttttctttag gcatccaact aatctacctc cattcttcca
    25201 tttaagtaag tttagaggta agagattatt ttaaattgtt tacattatct ttgaagtctt
    25261 ttcctcttac ttcaaaactt tcatacatat cccaacttgc acaagctggt gaaagaagca
    25321 ctatatctcc actttttgaa atttcataag aagtcttaac tgcttcttcc atatctttta
    25381 ctatatgtat gtcattaaat cctttatttt tagcacaatt ttctatattt gatgcagttt
    25441 ctcctaataa gaccaatgat ttaacatatg actttgcagt ttcaagaagt tcatcaaaat
    25501 tacttccctt atccatcccc cctgctataa gtattatagg tctatcataa gactgtatag
    25561 cttttatagt ggaatctgga ttagttccct ttgaatcatt tacatatatt acattgtcta
    25621 aatttcttac aaactcttgt ctgtgttcca ctcctttaaa tgtcataagt acgtccttta
    25681 taacttctaa atcaattttg cagacatatg ctattgctat agctgccata caattttcta
    25741 agttatgacc tccaggtaaa cttacatcat ctttatttag aaatttgatt ttatcatcta
    25801 tatctattat tatgtcatta ttttctaaat atactcctcc atttactttt ttagttcttg
    25861 aaaagtaaat tactttggca ttgcatttat cagctaaaga ttttactatc tcatcatcat
    25921 agtttaatac acagaaatcc tcaacagttt ggttcataaa tatatttgcc tttgcttcta
    25981 tgtatttttc catagtatgg tgtctattta aatggtcctc tgtaatattt aatattgcgc
    26041 ttactttagg tctaaattca tctatactct caagctgaaa actgcttagt tcagttacta
    26101 acacagactc ttcacttgaa gtttctatag tatctattac aggatttcct atattaccaa
    26161 ctacatatgt atctcgtttt gctcttgaaa aaatttctcc taccaaacta gttgtagttg
    26221 tctttccatt tgtacctgta atccctataa aaattggatt attcgatagt ttaaaagcta
    26281 attctacctc accaattata tacttgcctg aattttttaa tttcaatatg aatggtaggt
    26341 ctagtggtac acctggagac actacaacca tatctatatc atctacatct tctgggtgat
    26401 gtcctaatat gtattttatg tcatttatag atttcaattc atcaagtata tttctcaatt
    26461 tattttcatc ttttatatca tttacgatta tacttgcacc caatttatct aaatgtttaa
    26521 ttgtagatat accagttttt gcaagtccta ctaataaaac tttttttcct tttaagtcca
    26581 tttttcattc cccctagccc taaaatactg ctattatact tatccaagct aaaacaactg
    26641 ttataatcca aaaaataaat acaactcttg tttctggcca accacactgt tcaaagtggt
    26701 ggtgtattgg agccatctta aagaatctct ttcttgttgc cttaaaatat ccaacttgaa
    26761 tcaaaactga tagagcctcc gcaaaataaa ttcctccaat tataggtatt ataagcactg
    26821 agttagttag aactgaaaat gctacaactg cacctcctaa tgccattgaa cctgtgtcgc
    26881 ccataaacac tctagccgga tatgaattaa atcctaagaa acctaaacat gctccaacag
    26941 ttgctgctgc taaaactgcg acttctgtat ttccagcaat agaacttgca aatagcataa
    27001 agaatacaga aactattaat gttactcctg atgctaatcc atctaatcca tcagtcaagt
    27061 ttacagcatt tactattgct acgattataa acatcattat tggtatatat aatattccaa
    27121 catttatcac aaaatctgta aatggtatca taagttgtga agcacttgat gatgatgtat
    27181 actgataaaa agcaacataa aaagataatg caacttgaag gattattttt tgatatgctt
    27241 ttagtccaag tgatcttttt agctttattt ttataaaatc atctaaaaag ccaataaaac
    27301 caaaacctgc aatacatata agacctactg ccatatcatg actaactttt actcttgtaa
    27361 gtccagttat tagaattgca acaatcatta taattccacc catagtaggt gttccgtttt
    27421 tagctagatg tgtttggggt ccatcatctc taactgtctg accaaattta aattttctaa
    27481 gcattggtat aaatattggt cctattataa ttactattaa aaacgcaatc aatgcggtgt
    27541 acgtaagctc tgttattcct aacatcataa aactcctctc ccttgtcaat taactcatta
    27601 gtttaagctt atttatttag atattcgact attttttcta atctcatgcc tcttgagcct
    27661 tttactaaaa tagtgtctcc tgtttttact aattcattta acttgttaaa cacatcttct
    27721 ctattctcaa agtgatatat attggctgaa ttgaatccta attgttttgc ttcttctcca
    27781 ataaacactg aattttcacc tatagttatt ataatatcag tattattcat agatgatttt
    27841 ccaactaatc tatgaccata ctctgaaatt tcacccattt ctagtatatc tccaagtatc
    27901 gctacccttc tacctttata tcttccaagt atctttaaag cagcatccat agaatctgga
    27961 ctagcattat atgaatcatt aattattgtt aatttttcat tttttattat gtctagtctc
    28021 atttttgtcc ctttaaaatt ttttaaacct ttttttatat aatctaaaga aatatttaga
    28081 cacaaaccaa ctaatatagc agacattgca ttatatatat tatgttctcc tattgttggt
    28141 ataaatattt cctctttttt cccatttatt acacatataa atgttaaact atcctcttcc
    28201 atagtataac tttcacaata aacatcattg tttttattaa agccaaatgt ttttaactta
    28261 tatacatgtt ctttttcttt taatgtagat aaaaacttat catctccatt tacaattaaa
    28321 gtatttgttt catcaaagtt tgaagtaatc tccatctttg ctttcaaaat accctcttgt
    28381 gaacctagtt tttctacatg tgataatcct atattcgata tgactgcaat tttaggattt
    28441 actatatcaa ctaaatattt tatttcattg aatccagaca tacccatctc tattacagca
    28501 cactcatgtt ctttatttag attaaataaa gtcaatggaa ctccaaaatg attatttaag
    28561 tttttttcat tttttagtat atttaacttt gcagatatgg cagagtaaat catatctcta
    28621 gtagtagttt ttcccacact acctgtaaca cctataaatg gaatatcaaa tttttcttta
    28681 tagtattttg aaatatctcc tagagccaaa gatgtatctt ttacctctat taaatttata
    28741 tccgaacttt ctaattttat tccattactt tcatttttta taaaagtttt acatccacta
    28801 tcatatgcag acttcataaa tgtatgccca tctaaatttt ctccaactat ggctacaaat
    28861 gcattttggg catttgcctc cctgctatct ataactatat cacttacaca gtcattataa
    28921 tctccatata ttaacttccc attagttgca agtactaact cttttattgt taagcactcc
    28981 attattaaac tcctccttta aaaccttagc tatattctct tagcaacaaa aggcaatgta
    29041 taccgttata tatacattgc ctacgataac tcttaacttt ataattatat actatatttc
    29101 tgtttttttg aagatgttta tgggtatatg ttaattatct gttacttttt agttttcaaa
    29161 atatagggtt atacttgagt cttcattaac ttttactcca ggttttggga atatatcctt
    29221 tacctttgta tcttctggaa gttctatatc tgcatctaag tctggtttta acttcgcatc
    29281 ctccaatgcc tttacagcat ctcctatttt tagatttctt acatctggca ctttaacttg
    29341 cttcttttca tactcagctt tttcttcttc tttatatact ggtttaactc ccaaatattt
    29401 taaagaatca ttcattattt ctttcactat tggtcctgct gtagtactac caaatgcact
    29461 tactcctgtt ggttcgtcaa caatagcaag aactactatt tgcgggtcat cacaaggtgc
    29521 tattccaaca aatgaacata tatactttcc tggagcatac ttaccatcta taactttttg
    29581 agctgtacct gttttaccac caagtctata acctggtata tatgctatct ttcctccacc
    29641 ttcagttaca actgattctg caatttctaa cattttttta gatgtttctt tagatataac
    29701 acttcttact ttttttggtt ttactgtttc agttatattt cctttattgt cagtataaga
    29761 ttttaccact cttggttgca ttaaatctcc accattagct attgatgata tagctgttat
    29821 aagttgtatt ggagttactg atatagattg accaaatgat attgtagcca attcaactgg
    29881 tcctacattt ttttcgttat acagtattcc cttagcttct cctggtaaat ctatacctgt
    29941 cttatccata agaccaaatg attctatata atcatacatt tttccaacac ctaatctgct
    30001 tcctaactca acaaatactg ggttgcaaga gttttgtact gcttgtttaa attcttgtgt
    30061 tccatgaggt ctgtaatgtc tccaacattt aatctttctt cctccaacag ttacactacc
    30121 tgtacatgtg aatttttctc catctttaat tactccttct tcaagagcac tggaagatgt
    30181 aataagctta aatgtagaac ctggttcata tgtatcactt actgctggat ttctccacat
    30241 ttgataatag ccttttattt tatctttatc attatatttt tcaagttctt cttgataata
    30301 tggatatatt ggcgttcttg agtcattagg gtcataatct ggttttgatg ctaaagcaag
    30361 tatatcacct gttttagggt tcatagctat tgcagtaact ttttttgcat tatttaactc
    30421 ataagctttc tgtactgctt tttcagtata gtgttgaatt acttcatcta tagacaacac
    30481 aagaccatta ccttgtacag gctcataata tttttccatt ccttgtggta tttctcttcc
    30541 tgaagcatca gtacttacta taagttttcc aggctttcct tttaactttt tatcatattg
    30601 catttctaca ccagatatac ctgttgcatc tgaagaagta tgtcctaaca catatggtgc
    30661 aaaattccca tatgggtaat atctttgatt atcttctgcc acccatattc cacttaattt
    30721 agcatctctt atttgacttg ccttatcatc atctatccat ctttttactt ttacaagagc
    30781 catatttttt ttgcttatta aagcgtaaat atctttataa tcttcatcca aaatctcagc
    30841 taccttttcg gctgcttctt ttttatcttc tacttccaca ggtttacacc aaacagtata
    30901 cttagttaca cttactgcta actctttcat atttctatca tatatagttc ctcttttggg
    30961 ttctattggt atatctcttg tttgttgttc tagtgctttt gtactcaacc aatttccctt
    31021 cataagttgt agatacccag tcctaataac aaggcaaaaa aacaatgcac atgctagaat
    31081 aagaacgagt accagccttt tcttacttat cctctttact tttctcaaat ctattccccc
    31141 tagttatcca catctatata ctttatttgt ccatcttttg gatagtccat attgagcaat
    31201 tccttagcct ttagctcaat ttcttttatt gatgtttttt cagtttcttt tgcctttagt
    31261 tcctctaata ctatctcttt ttttcttaaa tctttttgta aataatagat atcgtacttc
    31321 atttccgaga tttttgaata accacataga ttcaatatta ttactgaaaa agttgcaatc
    31381 agtaaaatgc atttagctcc tatttttttc ttatttctct ttttataatt attctttttt
    31441 ttatttttat attcatttag attatgtatt ttataatttt ttttcaaatt ctcaccccga
    31501 tagtatatag tttaaatttt cctctatact atatttgtta ttaatcatat ttcttcctct
    31561 aaaatttcat cgctaaatta atacactatt acacaatcta attgtatttt tttctataca
    31621 caacaaaaac ctataaattc acttatactt ttatatgaat ctataggtta gatattaatt
    31681 tctatttatt tttatcactt ctagtatttt tagataattt cttccataaa tataatatgt
    31741 aactaactat ctactctctg attcctaagt tctccattat attacttaat accatctata
    31801 tctatatttt tatatctaaa ttttcattat atatctcaat aacaaagaac atttcttata
    31861 gtgttaattg ttttttaatt gctttaccag tgcaacaaaa tatataatac tttttaatta
    31921 attataataa tcttatatat aagattattc tttagcctta taactactta tattttttct
    31981 gctactctta gcttagcact tcttgaacgt ggatttactt ctatttcttc ctcactaggt
    32041 aatataggtt ttctagttat tattttcacc tcagattctt tatcacattg gcatataggt
    32101 aaatgttgtg gacatataca atctgatgct aaatgtttga atgcattttt cactattcta
    32161 tcttctaatg aatggaatgt tattatacat attcttccac cttcattcat tatagaagaa
    32221 gcatcattta tcatctttgt aattactcca agttcattat ttacttctat tcgtattgcc
    32281 tgaaaagttc ttttagctgg gtgaggtcca tctattctag ctttcttagg gatagctttt
    32341 ttaattacat ctactaattc tcctgtagtt tctattggtt tgtttgctct ttcttcaact
    32401 ataaatttag ctatacgctt agcccagtta tcttctccat aatcttttat tatcttagtt
    32461 aattcatctt ctgtataagt atttacaaca tcatatgcag aaaactcaca cctaacatcc
    32521 attctcatat caagtggtgc atcttgcata taagaaaatc ctctatcagc ttcatcaagt
    32581 tgatgagatg aaactcctaa atcagctaat acaccatcaa ttttgtaaac tcctattttt
    32641 tctaattcct cttttatatt ttcaaaattg ctgtgaacaa acttcactct actttcatat
    32701 tcactcaatc tctcttttgc tgtgcttata gcatttttat cttggtcaaa ccctataaat
    32761 aatcccttat ctgacagttt ttttactatt tctttagaat gtcctgctcc tcccatagta
    32821 cagtcaacat acacgccatc tggctttata tttaaattct ctatacactc atttaaaaga
    32881 actgatacat gatgaaattc catagttacc tcccttaaat tcacttttta aactattctt
    32941 cactaatatt atttttattt ttctttgata aataaacatg tgcaattatg ccaccaacaa
    33001 ctccaattag acttataact tgtgccattc taagtggtcc taacattaaa ctatctgttc
    33061 ttaatccctc tataaaaaat cttcctattg aatataaagt tatataagta actataactt
    33121 gaccttcata ttttttattt tttctaaata gtaataaaac tataaatatt ccaaaatccc
    33181 atatagactc atataaaaaa gttggatgta cctttactcc atcaaccatt attccccaag
    33241 gtaaattggt agcacctcca tgtgcttctc cattaataaa atttccccat cttccaatag
    33301 cttgtgctaa aggcattcca agtataactg tatcagccat ttttaagaag ttaatatttt
    33361 taatcttagt gtatatataa ccagctaata ttccaccaat taaccctcca tgtatagcaa
    33421 gtccaccacc tctaaaatta aaaatttgag acatattttg agcatagtaa ctccaattaa
    33481 atacaacata atagattctt gcacagatta gtccaactgg aattgctatt atagctatat
    33541 ttaatacatc atcttcttta attcccactc tttttgcttc ttttattgct attaaagttc
    33601 ctaaaatcat tccacatgcc ataagtattc cataccacat gatgtctatg ccaaacagtg
    33661 taaatgctac tctatccata acgtcacctc tttgtattaa ttgaaaatat ttccaaagca
    33721 atatgctata attacaatta attgtaatta tactaaatta tgataacaca ttaatgttaa
    33781 ttctacaaaa aatattaaaa tttattctac aactccctac actatattat atctaattac
    33841 tataaaattt tttaaactga atctctcatg actaaagtta caagtgtaca tagatatttt
    33901 ttaatcaaaa agattgatgc atatatacat caacctcttc taattcttat ctatagctct
    33961 taaattttta cctcaattta tataaatata ttttataaat ttgttttatc aaaataaaca
    34021 tttaaaaaca taaactaata cttaattagt tattcttatt atgattaagt ttctatttag
    34081 gctctacaat agaaataaca caatactctt ctttaaaatg ctcttttaat ctttcttcaa
    34141 cttcctcaaa agtaatatct ttaataacat ctaaatactc caatagattt atatccttaa
    34201 aaacataaga tataaaacta ttacctataa agtttataga atcaaagcac ttaataaatg
    34261 accctatttt tttcttttta gttctctcaa actcttcttt tgataatcct tctttttttg
    34321 atttttctat ataatcaaga attatttctt ttactttttt aggctcttta gaatctccag
    34381 ctattatgct aaaagcataa tccacttgag acgaaaatcc agctccaaaa ttttcattta
    34441 ttaaaccttg catatacaaa tcttcataaa gcttacttcc ttttttaaat aacatcccta
    34501 caagtatatc agttacaatc tctttacgta aaagttcttt cccttttaaa ccaacattac
    34561 tatctttaaa acctatgttg aacataggca tagatatagg gaatttttct ataacctctt
    34621 tttcttttac accttctggt tcttctggat aaaatctttc tatactctta gagagtttat
    34681 ctactttgta attgtttgac tttttagtta catctataac tttttctaca tctaagtcac
    34741 caacaacaaa taaagccata tttcctggat tatagaaagt attataacat ttatatagct
    34801 cttccttagt tatcttatat atactatcaa cagtacctgc tatatcaatt ctagcaggat
    34861 aatttacata cattgccttc aagcaattaa agtatacatt ccaatcaggg tcatcattgt
    34921 acatttttat ctcttgtgct attatacctt tttccttttc aacattttca tctgtaaaat
    34981 aaggtgtttg aacatagtca attagatgtt ctaaactctc ataaaaattt tcagtagcag
    35041 aaaataagta ggctgtcatt gtaaaattag taaatgcatt tgcatttact cctaattttg
    35101 aaaatttatc aaatgcatct cctccatcag gttgctcaaa catcttatgc tctagaaaat
    35161 gtgcaatacc ttcattcact ctaattttct tatcttctcc aattggtaca aattctaagt
    35221 catttgaacc ataattagta gccaaaatag catacttttt cataaaaccc cttttaggca
    35281 taaaatatac atctaatcca ttttgcaatt tctcataata aacttcttct ttcaatatat
    35341 cattaactat tttttccata gacatctcct ccattaattt ctcaagaagt atacagtatc
    35401 aagctctata ttttttatag cttcaactat atcttttttt gtaacctttt ctatactaga
    35461 tattatgtct tgtacttcag aatttgtctt tgccatagat tgagagaaaa caaaatcaga
    35521 cataccacct atattatcag ttatagattt catagaatta actaaagcaa gtttactgtt
    35581 ttctaattct tcatcagata tatcacctgc ttttacactt tctacttgtt tttttatcaa
    35641 ttctacagct ttatcataat ctttagtttc tataccagat gatataaaca tgatactttt
    35701 atacttctct attgaagaga atatatagta acataaactt tctttttctc ttacattaac
    35761 aaataattta gagtgtggac caccacctaa gacattgctc ccaactacta aagcataata
    35821 tttatctatg tctgcataat ccacatttgc tctataaccc ataactaact taccttgagt
    35881 tatttccatt tgttcatcta taacttttac ctcatccaca ttttttataa aatctgctct
    35941 tggtatattt ataatttctt ctctttcaaa ttttagattt ttacttatta tatctacaac
    36001 tttatcttca tcaaaattac cttctacaac tatatctata ggtgatgttt taagtatgtt
    36061 tttataatgt tcatataact ctcttgaagt tatactatct atttcatctt cataaccaaa
    36121 ttcacttaca ctatatctct ctcctttaca catagcctca atacatttat cttgcgcata
    36181 ttccttttta tcatttatta ttgattgtat cttttctctt aaattctctt tttcaatatt
    36241 taaatattct tctttaaacc caccatctac aacaagcgta ttatttatta cttcattaaa
    36301 aaattctact acttcttcaa atatactctc atctaaatac ttttcactta tgttaattac
    36361 tttaaaactc aatacttgac gttctccacg ttttacagca tcagctccca tagatgaacc
    36421 atataaatca tctaatttat tggatattgc tctagcacta ggatatttag cgcttccact
    36481 tgctattatt gatggtatta gagcattttt tgtagtttct ttttcatcca atagtctctg
    36541 aacatataaa cttataagat tcgatttaaa cttttctgtt tttatcaaag taagattaat
    36601 attattacct aaatttatta tttttttagt attaaccata tgtacctcct atttttattt
    36661 aaacatcaat tttaaaattg aaatttattt attttttaat tacatatact cacatataaa
    36721 aaccttctat ttaattatat cctttttatt attttctaaa agcaatattt ttatttaaat
    36781 taaatacaaa tattatttaa atcaatatgc tttaaagtct agtttacaag cttgatatat
    36841 atgatgtata atcaatatgt atgtctataa ttaaaacaat tttgcaatcc tatagattga
    36901 ttgtttaaag ttatataaag aatataaaaa agaataagag agaggacgat taatatgaaa
    36961 ttaggtatag ttggtttacc aaatgtaggt aaaagcacac tatttaacgc aataactcaa
    37021 gcaggtgcag aatctgcaaa ttaccctttt tgtacaatag acccaaatgt gggagttgta
    37081 tctgttccag atgaaagatt aaacaaacta caagaattat ataattctga aaaaatagtt
    37141 cctactgcta tagaattttg tgatatagca ggtttagtta gaggagcttc taaaggagaa
    37201 ggtttaggaa ataaattctt atcacatata agagaagttg atgcaatagt tcatgttgtt
    37261 agatgttttg aggatgaaaa tgttgtacat gtagatggtt ctgtagaccc acttcgtgat
    37321 atagaaacta taaacttaga attaattttc tcagatatag agattcttga aagaagaatt
    37381 aataaaactc aaaaagctgc taaggctgat aaaactttag gttctgaact tgatttatta
    37441 aaatcaataa tgtctacttt agaagaaagt aaatgtgtaa gaactatgga atttacagaa
    37501 gatgaacaaa cattcgtaaa ttcattggat ttacttacat ctaagcctgt aatatatgct
    37561 tcaaatgtat cagaagatga tttagctgat aatggagaaa ataataaata tgttcaacaa
    37621 gttaaagcat ttgcagaaac agaagatgcc gaagttgttg tagtatgtgc acaaatagaa
    37681 gcagaaattt ctgaacttga ttctgctgaa gaaaagaaag agtttttaga aacattaggt
    37741 cttgaacaat cagggcttga taagcttata aaatcttctt atgctttact tggtttaatt
    37801 tcattcttaa ctgctggtcc aaaagaagtt agagcatgga ctataaaagt tggaagtaaa
    37861 gctcctcagg caggaggaaa aatacattct gatatagaaa gaggatttat cagagcagaa
    37921 acaatagcct ttaatgattt ggttgagcat ggtactatgg ctgctgctaa agaaaaaggg
    37981 ttggttaaat tagaaggtaa agaatacata gttaaagatg gagatgtaat attatttaga
    38041 tttaatgttt aaaatgatta atcgtataca ctttgactat tatatataat aactaaaaaa
    38101 ctaagattat aataatacaa tattatctta taataattat atagaattta aaaataaaag
    38161 aagagtattt caaatgattt gtataaatta ttatgaaaaa ctcttttttt gatataataa
    38221 aattacttac atatataata tttaaattta taaatta
//
LOCUS       DAGSWI010000025        37724 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.025, whole genome shotgun sequence.
ACCESSION   DAGSWI010000025 DAGSWI010000000
VERSION     DAGSWI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.025"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            188..904
                     /locus_tag="MJF80_001149"
     CDS             188..904
                     /locus_tag="MJF80_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0279821.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            879..3212
                     /locus_tag="MJF80_001150"
     CDS             879..3212
                     /locus_tag="MJF80_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBZ0279822.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKVINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKAISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTMTGMFFVWLKKAGKTVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(3529..6207)
                     /gene="mngB"
                     /locus_tag="MJF80_001151"
     CDS             complement(3529..6207)
                     /gene="mngB"
                     /locus_tag="MJF80_001151"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBZ0279823.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEYSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(6282..7388)
                     /locus_tag="MJF80_001152"
     CDS             complement(6282..7388)
                     /locus_tag="MJF80_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBZ0279824.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENIEFDEIELDIM"
     gene            complement(7502..7810)
                     /locus_tag="MJF80_001153"
     CDS             complement(7502..7810)
                     /locus_tag="MJF80_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBZ0279825.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            complement(7813..8265)
                     /locus_tag="MJF80_001154"
     CDS             complement(7813..8265)
                     /locus_tag="MJF80_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0279826.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            8499..10427
                     /locus_tag="MJF80_001155"
     CDS             8499..10427
                     /locus_tag="MJF80_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBZ0279827.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILDEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            complement(10687..11448)
                     /locus_tag="MJF80_001156"
     CDS             complement(10687..11448)
                     /locus_tag="MJF80_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0279828.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            11667..12143
                     /locus_tag="MJF80_001157"
     CDS             11667..12143
                     /locus_tag="MJF80_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBZ0279829.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            12241..12393
                     /locus_tag="MJF80_001158"
     CDS             12241..12393
                     /locus_tag="MJF80_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBZ0279830.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(12553..12741)
                     /locus_tag="MJF80_001159"
     CDS             complement(12553..12741)
                     /locus_tag="MJF80_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBZ0279831.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            13102..13452
                     /locus_tag="MJF80_001160"
     CDS             13102..13452
                     /locus_tag="MJF80_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBZ0279832.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            13471..15114
                     /locus_tag="MJF80_001161"
     CDS             13471..15114
                     /locus_tag="MJF80_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBZ0279833.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFTKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            15195..17252
                     /gene="recG"
                     /locus_tag="MJF80_001162"
     CDS             15195..17252
                     /gene="recG"
                     /locus_tag="MJF80_001162"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBZ0279834.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            17311..17865
                     /gene="rsmD"
                     /locus_tag="MJF80_001163"
     CDS             17311..17865
                     /gene="rsmD"
                     /locus_tag="MJF80_001163"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBZ0279835.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            17867..18364
                     /gene="coaD"
                     /locus_tag="MJF80_001164"
     CDS             17867..18364
                     /gene="coaD"
                     /locus_tag="MJF80_001164"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBZ0279836.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            18368..18892
                     /locus_tag="MJF80_001165"
     CDS             18368..18892
                     /locus_tag="MJF80_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279837.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            19236..21155
                     /locus_tag="MJF80_001166"
     CDS             19236..21155
                     /locus_tag="MJF80_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBZ0279838.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINKQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            21168..21455
                     /locus_tag="MJF80_001167"
     CDS             21168..21455
                     /locus_tag="MJF80_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0279839.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            21588..22847
                     /locus_tag="MJF80_001168"
     CDS             21588..22847
                     /locus_tag="MJF80_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBZ0279840.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            22850..23590
                     /locus_tag="MJF80_001169"
     CDS             22850..23590
                     /locus_tag="MJF80_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBZ0279841.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQILVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            23913..25223
                     /locus_tag="MJF80_001170"
     CDS             23913..25223
                     /locus_tag="MJF80_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBZ0279842.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            25321..26238
                     /locus_tag="MJF80_001171"
     CDS             25321..26238
                     /locus_tag="MJF80_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBZ0279843.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            26254..27063
                     /locus_tag="MJF80_001172"
     CDS             26254..27063
                     /locus_tag="MJF80_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBZ0279844.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            27121..28869
                     /locus_tag="MJF80_001173"
     CDS             27121..28869
                     /locus_tag="MJF80_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBZ0279845.1"
                     /translation="MEISYVLKDCTFKHNKYSVIEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            29131..30153
                     /locus_tag="MJF80_001174"
     CDS             29131..30153
                     /locus_tag="MJF80_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBZ0279846.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGVTKEAPETSRRAWVKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDREILS
                     ALIEKR"
     gene            30158..32464
                     /locus_tag="MJF80_001175"
     CDS             30158..32464
                     /locus_tag="MJF80_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBZ0279847.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            32436..33551
                     /locus_tag="MJF80_001176"
     CDS             32436..33551
                     /locus_tag="MJF80_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279848.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVRIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            33569..34987
                     /locus_tag="MJF80_001177"
     CDS             33569..34987
                     /locus_tag="MJF80_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBZ0279849.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            35002..36210
                     /locus_tag="MJF80_001178"
     CDS             35002..36210
                     /locus_tag="MJF80_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279850.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLNLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(36284..37672)
                     /locus_tag="MJF80_001179"
     CDS             complement(36284..37672)
                     /locus_tag="MJF80_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBZ0279851.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
BASE COUNT        14309 a         4333 c         6236 g        12846 t
ORIGIN      
        1 atattaagta tataaaacaa aatgaaaaaa tattaaattt aaactagaag gaggtttcta
       61 agatggacat taaatacaaa taaccagaac aaaagatata aatatatata taaatggaaa
      121 aacttctatc atattgtata atcatatatg taaagaaaat ttataaatta atataaagga
      181 gattagaatg aattcatata atattttggt ggtagaagat gaaaaggaga tagcagatgc
      241 tatagaaata tatctattaa atcaaggata taatgttttt aagggataca atggattaga
      301 aggtcttaag gttatagaaa accaagaaat acatttagct atagttgata taatgatgcc
      361 taaaatggat ggtataacac ttacaatgaa gcttagagaa aactataatt ttccagtaat
      421 aatgctatct gcaaaatcag aggaagttga caaaataatg gggcttaata taggagcaga
      481 cgattatgta acaaaacctt ttaagccatt agaactattg gctagagtta attcacaact
      541 tagaaggtac actaagtatc taaacatggt tggaaataaa gagcaaaagg tagaaagtga
      601 cgatagtgtg tttgcaatag gcggattaga attaaatgaa aatacaaaag aagttagtat
      661 tgatggaaag tatataaaaa caactcctat agaatttaaa atactaagtt tacttatgag
      721 aaatgcagga agagtttttt ctgctgatga aatatatgaa agagtttgga atgagaatgc
      781 tgtaaataca gatacagtta tggtacatgt gaggaatata agggaaaaga tagaaattga
      841 cccaaaaaat cctaaatatt taaaggtggt gtggggtgtt ggatacaaaa ttgaaaaaat
      901 ttaaagataa atatatagtt actattatag tttttatact tatgttatca gcatcattag
      961 gtatgattag tcaatattct actatacaag caagtgctaa aggtggagca gagaatccat
     1021 ttgaacagga aagctttgta gatagtatat ataaaggaag ttatgtttta tatcataata
     1081 tgaaggaaga acaagaaggt aagatgatac aaccatcaaa gctatttatc aatgaagaaa
     1141 ctatgaacta tataaaaaat gtaagtaaag aaaaagatac ttatgatggt tcagaaactt
     1201 actatacaga tgatagtatt aatgcagatt ttaatgaaaa ctttgaaaat tgggagcatt
     1261 tcattaaagc gtcaggaaag aatttaaagt actatttagt tgataaagaa agcaattttg
     1321 aattttttaa tgataataaa gaattaggag tacttgatac aacaaagaat gatgcttcaa
     1381 aagaaaattc tgatgaaaat cagtcaaaaa atacagaaat gaatgatgag gaaagacaaa
     1441 agcagattac agatataaaa gaaaactata gattctacgt tgtaatgaat tttgataaag
     1501 atggaaaagt aaatattgta gattcttatg gagtaaatca aaaagttatt aatcaaatgt
     1561 tgttatctaa agcaagagaa gatttaatgg atacaagttt aggaggaaat tcaatttcat
     1621 ataagttatc tccaataaaa aatataacat tagtatatgc tgtacctaaa gcaataagta
     1681 aaggtgatag tgtagagtat ggttatggaa atacttattt tgatgatggt ataatttcat
     1741 atataagcaa tgtagaaaat aattcttatt ataagatttc tggaatgatt ttaaaaatta
     1801 tattagctat agttgttatt gtagctttag cattcccata tagaaaatct aaagagttgc
     1861 taggatttga tacattatca agaataccgt tagagatact ctttattgct cttgctatta
     1921 tacaggggct tgtagattta tatcctactg ttataataag taataccttg agtgggaatt
     1981 atgtagagta ccttataaac aatgacataa gtgctaaaat ggctagcata ttggtaagtt
     2041 taatgaattt aacttattgg tttgtaatat tctcaataat atttgtattt ataatacttt
     2101 taaaacacat tttaaatgtt ggaataagag agtattttac aaaacatact atgacaggaa
     2161 tgttttttgt atggctaaaa aaagcaggaa aaacagttat taatcaaatt agaacaataa
     2221 atctaaaaga aaagccaaat aaaactatag ttacaatttt gggaataaat ttattgatta
     2281 tagttataat gtgtagtatg tggttctttg gaataatact cgcattaata tattcagtag
     2341 tattatttaa gatactgtca gattactcta aaaagacaat atatgaatat aatcaacttt
     2401 tagatgttac aaagaaaatt tcagatggaa acttagatgt aaatatggaa aaagatttag
     2461 gattttttaa tcctataaaa gacgaattag gaaatattca aactggattt aaaaaagctg
     2521 ttgacgaaga ggttaagagt caaaagatga aaacagagct tatatcaaat gtatcacatg
     2581 acttaaaaac acctctaaca tctataatca cttatataga tttattaaaa gatgaaagta
     2641 taacagatga aaatcgtaag ttgtatattg atacattaga cagaaagtca caaagattgc
     2701 aacatctaat agaagatttg tttgaagtaa gcaaggcaaa tagtggagat gtttatttga
     2761 atatagtaaa tgtggatatt gtatctctaa tgaagcagac acttttagaa ttagatgaca
     2821 aacttgcaga ttcatctcta atagtaaaga ataacttttc taatgaaaaa ataatattac
     2881 cattagatag tcagcgtaca tttagagtat ttgaaaatct tataataaat ataagcaaat
     2941 atgcaatgcc aaattcaaga gtgtatattg atattttaga aacagataat aaagtaaata
     3001 ttatgcttaa aaatatgtct gcatctgaga ttgattttag tgtagatgat ataatggaga
     3061 gatttgtaag aggagataaa tcacgtaaca cagaaggttc tggtcttggt ttagcgatag
     3121 caaaaagttt tatagaatta cagggtggta agatgaatat aagtgtggat ggtgatttat
     3181 ttaaagttac aattactttt aacaaaaatt agattagact tatttaataa ttataaagtc
     3241 tatagaataa aagagtctgg aaatatttat gtgtattctt taaataaaaa tttataggta
     3301 ttgttttaaa ataatttgta agcattattt caaaacagct tataaatgtt attttaaaac
     3361 agaatgttga tttatttaaa aaaggtgaat cctaatatac aaggttcacc tttttatttt
     3421 acaaaatgtt atttagtgtt acagagctta taaatagcat aatatctggg tttactatta
     3481 acatagtact agcaatttat tttgaaatct tgtataagct attttatatt atttaattac
     3541 tattgtttgt actttacaat gtgataaaga atttacctct acagttttag tatttacttt
     3601 aagcttgcta gtattatcat agatttcgtc gagcattgca ctataagcat cattgacatt
     3661 tttattgatg ttaagacttc ccttagcatc ctcatttttc ataccattga agattctaac
     3721 tacaattcca tctccttttt cttctttctt tattgcactt actgtgaaat ttgaattatt
     3781 tacattcatc aaactatatt cattttcata tatttgtttt tcgtcattga atgcaaatat
     3841 aagtctacca tttaaaaaat cagcatattc atatatttgt ataggtgtta agaatctctt
     3901 agctgtatta gctacatttg cttcatcaaa tttattgtta tatatgtaaa gaccaaagtc
     3961 aaagtttaac tctttaagta attgtgcatc tggtgttgct attatttttt ctccagaagc
     4021 tcttcctggt ctataaagta aattttctct tcccatatat ccaaatgacc tgaatagtgt
     4081 taatgctatt gtatctaaat tatcaccaac tatttgatat tctcttacac aaccagttat
     4141 taatgctatt cctctttgtc catcctctaa agtaacaaaa ctttgcattg gttcgatagt
     4201 tataggtttt tcttgccatg agtaattgtc tcttttccac aatgcaaggt ctttttctaa
     4261 tactgttggt ctttgtatta ttccaaattg ttgatctgct atagaaaact tagatgctat
     4321 ttctgtatta aataaaactc ttactctatg agatagtgct tggttatttt ttatattaac
     4381 attaaactta attatttgtt ctttaggttc taaggtaatt ttcattgtta ctggcatttt
     4441 cacagaaact ttacctatag ccctttcttc cagtgaagct ggtatggtca atgaatattt
     4501 aagtatcatt tcttcttgaa tgtttgattt tagcacttct actgaagata cagaatctaa
     4561 agatgaaaca taaatatctt ttcttggagg agaatagttg tatgaatctc catcatctcc
     4621 attatcttca attacagctt ggtttttata agtcttatta gattctttat ctaatatttc
     4681 taatgaacca tttttattgg ctgttatttt gtagtatttg ttttctattt ctttgcaatt
     4741 acttgtttct atttcacatt tatttttagt atccaattca aaaactagtt gagtatatcc
     4801 cattggtggt atatctttta ctgaaagtgc aatttttgct ctatatacag ttttgggtat
     4861 atatattttt ttacttgggt ttaatcttat tgtttgagtt aatatataat ttgtaatatc
     4921 ttctttttct tttattatat aattaagaat ttctccattt ttattcttta tcacaaaatc
     4981 ttgacctgga atataagtat caaattctac aacttcattt ctgacttctg ggaatccatt
     5041 gaatactgtt aatgttattt cttcattatt atttttgatt tgagtactta taagtctcat
     5101 atgcaaatct aataaattta tactcaaatc tctggcttgt ttgtatcttg tgtatacatc
     5161 ctcatttgtt gtgtcactat tacaagaacc tatgctgtca tgagctgcat tttcaaacat
     5221 aagtttccat atatctttta taactagatg agggtattca tttccaagtg aataacttaa
     5281 tgttaataat ggttcaagta catttgtaat ataattttct aatttgttat tcattatttt
     5341 aaggtctgac cttgaggaga atatggattt gtgtattctc atatgtttag catttaaaaa
     5401 ttcaccttta agctctgtaa attcatttct ttcatttctt actgaatcta tatagttttc
     5461 tatatcactt atttcgtatt cattttcatt atctatttca ttagcttttt ttaatatatc
     5521 aggaaggttt tttcttattg gtgcttggtc aaatccattt ggaaaatata cgttattagt
     5581 agaagcttta accttaagtt ttccgattat atcttttaaa tattctttta aatctgaatc
     5641 atcttctggt atattacctc catagtaata accaaatggt atttgaactg ctaatacttt
     5701 tgaaccatca gaacctttcc atataaactc tgttgtatct accatatcat cagaaactcc
     5761 acgccaaaat aatgaactat ctattccaaa acctctgtat atttgtggca tttgagctga
     5821 ttgcccaaaa gaatctggta catatccaac cctcataggt tcaccaaatt cattgcatct
     5881 atctattcca tagtaaaggt ttctaattat agattctcct gatataacca attggtctgt
     5941 ttgagtatac caaggtccaa taactaatct tcttctttga actaaatctc ttattctata
     6001 ttcatcctga ggtctccatt ttaaataatc atctaagaga gaagcttgtg catccatagt
     6061 aaagtattta aagtcttctt tttcttccaa tatgtctaat atatcttgaa aatctttcat
     6121 taaatatatt tttgaccttg atgttgtaaa ataccattct ctatcccaat gactgtgtgg
     6181 aactacatgt acttttgtct ttttcataaa aatcctccca aaatttaatt gagaattgag
     6241 agtacactca attctcaatt atctaaaatt aagtttttaa tttacattat atctaactct
     6301 atttcatcaa attctatatt ttccgtagtt tcatcttcat tgaagtctac taaagttgtt
     6361 gatagtattg ctattactaa agctccaaat attgcaccta ctaagtaagc ccatccagaa
     6421 cctattgata taaatccata taatccacca actggaggca ttatagcatg tacacctaac
     6481 aaagctgata atcctacacc caaagcgcat cctatcatat taacaggaac taattttaat
     6541 ggatttgtta aagtgaaagg aattgcacct tctgttattc caattatacc cataactata
     6601 ttgccttttc ctgtttcagc taatgcattg gaaaattttg attttcttat gaatgtagct
     6661 aaaccatatc ctagtggagg tataactatt gcgcagttta ccaatatcgc tggtagatag
     6721 attccctcta acaataatgc atttcctgcc atccaagctg ctttatttac tggtccacct
     6781 aaatcgaatc ctatcattgc gcctaaaatt aaagccaata ccaccgaatt tgttccattt
     6841 tgacacattt cagctaccca agcatttaat gaagtattta tgtttcctaa aggtcctgct
     6901 aatacaacac tcattaaaaa accagttaat ccaactgtta taagtggcat tattataagt
     6961 ggcatcattc ctgatgctga cccagatatt ttaatttttt tctttaaaaa cttacaaaca
     7021 tacccagcaa agaaacctgc tattactgct cctaagaatc ctgcattagt attacttgct
     7081 atgagcccac caataaaacc tgcacccata gctggtttct cacctattga aaatgctata
     7141 aatcctgcta ataccgtatt taatagacct atgccagtcc aaccaacttg ttccattaag
     7201 taaagaatat gataaaaacc agatacattt ttataagcat ctaaacttgt tatacccact
     7261 gctaaagctg taagctttgg gatagctaca actaaagatg agcctattac aagtggcaac
     7321 atatatgaga tacctgtcat taagtgtccc ttaagttcac ttaaaagaga tttatttttt
     7381 ttactcattt ttaatcctcc tatataagtt tgttatattt tttgataata tgttataatt
     7441 atattagaca attttttaat ttttgatttt tctcattcta tattagagtg agattttttt
     7501 attaagaaat agcttctata cactttgtta agacagcttc tggttttttt aacgctatac
     7561 ctattttgac ttttatcact tttttaccat caaatctttc cattccagat atagtttggt
     7621 cagatgctat aagtataaag tccgcatctt gtgcttcttc ttttgttata gtatttactc
     7681 gtcccatagc accttgttgt tctactttga tttcatgacc catttttgat gcagcttgtt
     7741 ctaaagcttt tgctgccata ggtgtgtgtg ctaatcctgc tatacatgat gttattgcta
     7801 ctattttcat aattattcct ccttcatttt attagttata tattttacta aatgacttgc
     7861 atcactagca tttttaactt cacttgtaaa atcatcctcc attaaacatg tggctaattt
     7921 agatagagtt ttcaaatgta tatttccttc atttttatgt ggggctaaca aactaaatac
     7981 atatctaacc ttactatcat ctaaagtttt ccactctaac tcgttagttg ttttaatata
     8041 gagtatggaa gcttccttta catttacact ctttgtatga ggtatcgcaa atccatcttt
     8101 tattcctgtt ggaaattcat tttctctttt tagaaggtct tttaaaagag cctctttatt
     8161 agttgtaata cctagtatat aagcactatc tgcaataaaa tttaatactt cttctttgct
     8221 ttttaagtct ttatctataa atatatattt ttcttgtagt accatatatt tttccactcc
     8281 attcataatg tcttaactta tatttatatt gtaatcatta tcctaattaa attaaatata
     8341 actaatttcc acttcgacat ggaaatttat attatgataa taactaattt ccatatagaa
     8401 gtggaaataa attattataa tacaatttta ttagagtaat tgtattatat tagtgtaaat
     8461 agtataattt agaagaaatt ctataagggg ggatatttat gcacaagaga ttgctcacgt
     8521 tgtttaaatt attaaatgaa agtgatgata aaattacttg taaaacttta agtaatcatt
     8581 taaaagtgag tgagagaact ataagaaatg atattacttc tataaatgga actttagaga
     8641 aaaatggtgc tattattaaa ataaaaaaag gagaaggata ttatatagat attcttaatt
     8701 tagcattata ccaacagtat ctagcattaa tatctgatga tattatggat tcaagtgaaa
     8761 taccagattc tcctatagaa agaaatcaat atattttaaa atatattctc tataacaata
     8821 catatataaa actagaagat ttagctaata gcttgtatgt gagtaaattt acaatactaa
     8881 atgacataaa aagaataaag cctattttgt caaagtataa tttaatatta gtttctaagc
     8941 catattatgg agttaaagtt gaagggaagg aaatagatat tcgaagatgt atttctaata
     9001 atatgataaa taggaatttt gaaaactata taattggaat tacagataga gaaatagaat
     9061 tatttaataa tgtagattta atagagcttc aaagggtagt gttaagtgaa attaataaat
     9121 ttaatatcaa tttcctagat tttaatctaa aaaactttat tatccattta gctattactg
     9181 taagtagaat acttgatggt tactgcttag ataatgtttt agatgttgtt ttaacagatt
     9241 ttcaatcgaa tacaactgtt gaaaatatat ttaattatat tgagagcaaa tatactataa
     9301 ttatttcaaa ggcagacaga gtttatctat ataatcattt tataactaaa agttctcttt
     9361 tggataacgt ttccaatagg gtagatacta aaattataga gtatgttgaa gaaattttag
     9421 aagtaatcaa caatcaatat acttttgacc ttagaaatga tagtgtatta tttgatgatt
     9481 tagtactgca ctttaaatca attctcaatt ctaaatctta taatttaaat aaggtgaatc
     9541 cccttataaa tacaataaag agtaattatc ctttagcatt tgaaataact ttaaatgcta
     9601 tagaaaaagt ttttaaaaat agtatttact ctttaacaga agatgaaatt ggatatgtat
     9661 ccctgcatat aggtgctgga atagagagat tttttcaaag taatattaag tgtaaaaacg
     9721 tagtattagt atgtggttca gggtatggtt caagtaggtt attagaggtt caattaaaca
     9781 aagtttttca tgataaaata aatatacttc aatgtctatc atttaatcaa tttttagcta
     9841 gtgaattgtc agatgtagat ataataatat cgacaattcc tttaaatcat gatagtattc
     9901 ctattgtatt agtggattta aaactattaa aaaaagatat agagaatata tctaagtcaa
     9961 tcactaataa ttcccatatt tatagtaatc ttttggacaa tttctttgat aaaaatttat
    10021 ttattgttaa tcctaaaatc aaagataaag atgagcttat aaagttgatg tgcaacaaat
    10081 taactcaaag tgaaattgta tttccatcat ttgctgaatc tgtattttac agagaaagtt
    10141 tatcttctac caatatagat gattttttag ctatacctca tcctatggaa ttaagctcta
    10201 taagaactaa gatttgtatt tccatattgg atgaaccaat atattggagt gaagattcta
    10261 ctgttaaatt aatcatgatg ttggctataa ataaagatga ttatataaaa ataaactcca
    10321 tttatgatat ccttttaaag ataattcatg acaatgatat tagagatagt atatctaatt
    10381 gtaatgattt tgataatttt ctttccataa tcaagtctat agtatagatt tttatattac
    10441 ataagtttta aaaactttaa taaaatattt tttgttttta gcataaatag tataagtaag
    10501 ttttgtataa tttgttatta ttttgtattg attataatta ttatacaata aagattctgt
    10561 ctcaaaatag agagagaaac ctgtttttca ttgataaaat gataaaaata tatctgattt
    10621 catacaaaaa agagtgtctc atgaactaaa aagttcattt tgagacacca cctttattat
    10681 tttatattat ttatctcttg tatatattac tatagcatcc cttaaaaact tagctaatcc
    10741 aggttgttct ctatcataat atgatgtaaa acgttcatca tatacataca tctgtgctag
    10801 agaggaatga gcttctttag agtatgtgtt ccaagtgtaa ctcaaccatt tatgatgtaa
    10861 ttttgcaact tcctgagcca gttcactaga ggcatcacca gttttaaaag cttttttaag
    10921 tttactaatt atttcgatat ttaggttttg ccaattttca taatcttgct tagacatatt
    10981 tttaagtttt ttgtttgatg tatctataat atctttacca tatttctctc taatttcagt
    11041 tccataattt ttttcatttt catcaatcat cttttcttta aaaccttcaa atttttctat
    11101 atctgtcata gtgtattttc cctccttcag agctatagtt ttagttacat tttctattag
    11161 taaatcaatt tgctttcttt tggctagtaa tttattatgg tgttctttta acgcacttaa
    11221 actatcgaag tttggtgaat ttataatgtt ttttatattt tctaagttaa ttcctaattc
    11281 tctataaaaa agtatttgtt gaagtttatt aacttcattt tgaccatata tacgataacc
    11341 tgaagaattt attttaagtg gtttaagaag acctatttca tcataatatc taagtgttct
    11401 agtacttata ccagctagtt tacttaattt ttgaactgta tattccataa gtagtacctc
    11461 ctataaatta aatatacact tttacgcaac gtaaatgtca atacattaaa atataaaaat
    11521 ttatatcttt taataaaaat ataaaaaaat atctttactg gatattttac acacaactgt
    11581 caatactcac taatgaaaat taaaaaataa aatttataga ctatctttac taaaaaatag
    11641 agggtctata gtctaggagg aactgtatga atttaaaaag aagcaatgaa aaacgagaag
    11701 agtacagaag aatgtattca gataaagaaa gttttttaag tttgattcaa aattttacaa
    11761 gtgtctcaat agcaaaagaa ttaactctta aaaatataga gttagaaaca tcttttatat
    11821 gtgaatataa aggaaaagaa gttgatataa tttataaggt attttctaaa agcggcaaag
    11881 tatcacatta tatagtgtta gaatttcaaa cagaaatgga tactgaaatt gtcccaagat
    11941 tgaaatcgta tagagaacaa atttggaaaa gttttattat gaaaaaaagc cttgaagaaa
    12001 tagaagataa aaattttaaa cttcctaaag ttataccagt tgttttatat agtggacctg
    12061 aaaggttaag tagaaaaaaa ggaattttgg atattgtaga atctatatcg gagttcagta
    12121 ataattctag tatgaaaaat taaaattaca taatgtgttt attggtctaa aatggaaaac
    12181 tgtcgcataa acttgaatat gaaaatattt atagatacag gttctatgtg gaggtgttcc
    12241 atgagaaata acaagtctaa ggtattgcta ggaatacttt gtctagcaac aggaatggct
    12301 gtaattctgt caatgttgct tcctggctgg atttggtcag cacttaccgc attaatactt
    12361 attggatgtg gagcattatt atttttttgt taaaatagtt ttaactataa agtgtggggt
    12421 gagtattgat gaaagtagta acaataaaat taccaaaatg gatttcaagt ataatcctaa
    12481 aatttatgag aaagagtaaa aatgattaaa ttaaaaagcc tacttacgga agtaggcttt
    12541 taaacgtggt tactaagctc tttcaatttt accagaacgt aaacatctag tacaaacttt
    12601 tactctctta ggagctccat ctataatcgc tcttacgctt cttaagttag ctgaccatgt
    12661 tcttttatta tgtttatttg agtgtgatac ttggtttcca gaaaccttac ctttaccaca
    12721 tacgctacat acttttgcca tctacgcaca cctccttaaa atgataacta tttaaagact
    12781 aaaaacatat ttatattatc ataaataaca aaaatttgca atagaaatat atatttgcct
    12841 tacacatatt gaagaatttc ttattatcat ataaaattat atatataatt ttatataaag
    12901 taaatatatt tggaaaataa ttattatttt tctttgaata tatatactat agaagaaatt
    12961 ttatccaaaa acttttctag gtttaaatta taccatattt atagtatgat atgattataa
    13021 ttttatataa taaataagta aaaatagtaa aaaataaatt aagaaatgtt tttaataaaa
    13081 ataagcttaa gggggtcaac catgagtgca aaggtaacga atcaatatgg aaatatagaa
    13141 atagataatc aagtaatagc tcaagtcgta tatagagctg caatggaaag ttatggctta
    13201 gtaggtttag catataagtc taaaggaata gtagagctat taaaaggcga gaacgcaact
    13261 aaaggtgtaa gagttgagga agtagaagaa gatgccatag caatcgaact ttatgttata
    13321 atacaatatg gtacaaatat atctactgtt gccaataata taatagatag agtaaaatat
    13381 gtagttgaaa aaatgactgg cgtaagagtt actagaattg atattaatgt acaaggaata
    13441 agagttaagt aatttcatag gaggaaacaa atgattcaat atatagatgg taaaaggtta
    13501 agggagatgt ttatttcagg tgcaaacaat ctccaaaaca ataaagagtt agttgacaag
    13561 ttaaatgtat tccctgtacc agatggagat acaggaacta atatgtcatt aacaatatct
    13621 tatgccttaa aagaattagc aaaagttgaa aatgataata tatcagatat cggaaaagca
    13681 ttatctaaag gttctttaat gggagctaga ggaaactcag gagttatact ttcacaaata
    13741 atcagaggta tagctaaatc tatagaagga aaaagtaaat tatcaacaga agatttagca
    13801 aaagctttta aaaatggttc agacacagca tataaagctg taataaaacc aattgaagga
    13861 actatactta ctgtagttag agaaagtgga gagtttgcaa taaaaactgc gaaaaaagag
    13921 aaagatgtag taaagttttt aagtatgctt gtgaaggaat caaattcttc attagaaaga
    13981 actccagacc ttctaaagaa tctaaaagaa gctggtgtgg ttgactctgg tggaaagggt
    14041 cttgtactta tatatgaagg aatgttagct tctataaaag gcgataacat agaaataaaa
    14101 aatgttgacc tagatactaa tatttcaaca agtatggatt ttacaaagag tactactagc
    14161 acagatgata taaaatattg ctattgtaca gaatttattt tagaaagttc taaggtagaa
    14221 gatactaaaa taagagacat aatgatggct tatggtgata gcctagcagt agttggagat
    14281 gatggagtca taaaggtaca tgtacacact aatgacccag gaaatgtact tcaagaagct
    14341 ctaaagtacg gtcaattatt aactataaaa atagaaaata tgaagttaca acatgaaaat
    14401 acattactgg atgtagaaga aaagaaagaa aatgatagtg aacctttaga agaagaaaaa
    14461 gagtttgggt ttatagctac ttctatgggt gaaggtttgg caaatatatt taaagatttc
    14521 ggagttgacc atataataga aggtggtcaa actatgaatc caagtactga agattttatg
    14581 aatgctataa agcatataaa tgctaaaaat atatttattt tcccaaataa cagcaatata
    14641 ataatggctg ctaatcaggc aaaagaattg agtgataaaa atataatagt tatacctaca
    14701 aaaaatacgc ctcaaggatt tgctgcatta gtaactttta atggagaatt aagtgaagat
    14761 gaaaataaag aagctatgat gaatgcttta aactcagtta aatcaggtca agtaactttt
    14821 gctgttaggg atacagtgat gaacgaaatt gatgttaaag aaggtaatat aattggaatt
    14881 gcagaaggga atttattatc agcaggagat tatgtggatg aagtaacatc aaaccttatt
    14941 gaaaagttgg ttgatgaaga tactgctata ataacattat tctttggtga agatgttaca
    15001 gaatctcaag caaatgaact acgtacttct ttagaagaaa agtttgaaga tgtagatgta
    15061 gagttatatt atggaggaca acctctttat tattacttaa tttcagttga ataatgatta
    15121 taagaacctg tatgaccaag tagcttttat aaaattggtt gtacaggatt tttgtgtagt
    15181 aggtgatata tttgttggat ttatataaag atgttcaata tgttaaggga ataggaccta
    15241 aaaaagcaga taaattaaat aaactgggta tatttacact aaaagaccta ttgtattatt
    15301 ttccaagaca atttgaagat agaaataatc taaagaaaat agcacaatta gaagatggtg
    15361 agaaagttac gataaaagca gtaatatcaa gtataaatac atttagccca aaagaaggta
    15421 tgactttaac aaagattgat gtaaaagatg agacaggctc ggctaaatta gtgtttttta
    15481 ataagagtta tataaaaaat acttttcgtc caggagattc aatattagtt tttgggaagg
    15541 ttaagaaaaa atttaataac ttagaactta catcatgtga attagaatat cttactaatt
    15601 cacctaagaa tacatgtaga tttatgcctg tgtaccagct tacatacggt gttacaaata
    15661 aagaaattat gagtataatt agaacagtac ttgaagataa agaattaatt atacaagaat
    15721 atatgccaca aagaattata gaaaaatata ggctatgtag tatagatttt gcagttagaa
    15781 acatacattc accaagtagt aaggaatcat taaaaatagc tttatataga attgtatttg
    15841 aagaattact tatactacaa ctagggttgt ttgtatttaa aagtggcaga aataaagaag
    15901 atggaataaa gtttgaaaca agtaaagact taaaaaaaat aataagtgct ttaccattta
    15961 aactgactaa agctcaaaac agggcattag atgaaattat acaagatatg aatttagaga
    16021 aaataatgaa tagattggta caaggtgatg tagggagtgg taaaacagta gtagctctct
    16081 tagcacttgc aaattgtgtt ttaaatggtt atcagggcgc tttgatggca cctacagaga
    16141 tattagcagg gcaacattat atttcactta ctgagtctct aaaagatttt ggaataaatg
    16201 taggtctatt aataggtagt cttacaaaaa aacaaaagga tacagtattg gaacaaataa
    16261 agaataatga gattgatata ttaataggta cacatgcact aattgaagat aaggttgaat
    16321 ttaataatat tggtttagtt ataacagatg aacaacatag atttggtgtt atgcaaagga
    16381 gcaaactttc attgaaagga gcaaatcctg atatattagt aatgacagca acaccaatac
    16441 caagaacttt agcacttata ctctatggag atttagatat ttctattata gatgaacttc
    16501 ctccaggaag acagccaata gaaacaattg ctattgaaaa aagtaagcga gatagagcat
    16561 ataataacct tgtaagaaga gaggttgaat caggaagaca agtgtacatt gtatgtccct
    16621 tggtagaaga aagtgaagcc atagaagcca aatcagcagt tgaacttgtt gaggagttaa
    16681 gagcagaata ttttcacgac ttaaggcttg ggttgcttca tggtaaaatg aagtcaagtg
    16741 aaaaagatga agtaatgaga ctttttaaaa ataaagaaat agatatttta gtttcaacaa
    16801 cagtaataga agttggtgtc aatgtgccaa atgcaacatt aatgataata gaaaatgcag
    16861 aaagatttgg acttgcacaa ttacatcagc ttagagggcg agttggaaga ggtagtcata
    16921 aatcgtattg tgtactaatt tatgattcta aaacagatgt atgtagacaa agaatggcta
    16981 taatggaaga gaccaatgat ggttttaaga tttcagagaa ggatttagaa ataagaggac
    17041 ctggggaatt ttttgggaca aggcagcatg gtcttccaga acttaaggta gcaaacttgt
    17101 ttaagcatat aaaaatactg aagttagcac agcaagaagc tcgatatata ttgggtgaag
    17161 ataataatct acaattaaaa gaaaacatgg cactcaaaaa agaaattata gataaattta
    17221 aagatacatt gaaagaaatt tcattaaatt aattttaaat tgtgttaaaa tattactaat
    17281 gtaataatgt gctgaaggga gattgtcata ttgagagtaa tttcaggaaa agcgagagga
    17341 ttaaaactaa atactccaaa aaatgaagat gtaaggccaa ctacagatag agtaaaagag
    17401 tctctattta atatgataaa ttcatatata atggaaagtg aagttctgga tttatttgct
    17461 gggactggtt ctttaggaat agagtgctta tctaggggtg ccaagtcatg tacttttgta
    17521 gatatcagta aggaaagcat agatattgtt aagtccaata taaaaaaggc aagagttgaa
    17581 agtgaaagta ttattttaaa ccttgatttt aagactgcaa tagataaact taaattacaa
    17641 aatagtaaat ttgacataat ttttatggac cctccgtatt ataaaaatat gtttatagaa
    17701 gctattgaaa aaattgataa ttctaatcta ttgaacgaag atggaataat agttgtagaa
    17761 catgatacga atgatttatt tccagataag atatgtaaat tggagaaaac tagagataag
    17821 aaatatggaa atactacatt aactttttat aagatggagg cataatatgg aaaataaacc
    17881 tagaaaagct atatttgcag gaagttttga cccaattact aatggacatt tagatattat
    17941 ttgcagggct tcgaagttat ttgacgaatt acaaatagga gtattaaata atcctaataa
    18001 aaagggtcta tttagctttg atgaaagagt aaaacttata gaaaaaagta caagccattt
    18061 aaataatata aaagttgtga cttttgatgg tttattaata aattactgtc aggaaaatgg
    18121 gattggagca ttagttagag gtgtgagaag tggtgctgat gttgattatg aacttcagat
    18181 ggcacatatg aatagagagc ttaatccaga tatagagact ataattttac caagttgtac
    18241 aaaatattca tttataagtt catctttaat aaaagaggtt ttgctctttg atgctgatat
    18301 aaaaaatttg gttccaaaga ttgttttgga agaattaaag aaaaaaacta gtgggggtaa
    18361 ttgatatatg aagatagatt tagaaatgat tgaactattt gagcagttag aggagatatt
    18421 tagaagtgct tctacaatcc ctttctcaca taaatgcaca gtggataaag atgaggtttt
    18481 agcaataatt aatgatataa gaactttaat accagaagaa gtaacacagg cagtatggat
    18541 taataaagaa agaaataaaa taataagtca agctaagcaa gatgctacaa atatagttga
    18601 acaagcgaaa aaagaagctg agagaataca acaagaatat caagataata tagaggaatt
    18661 aaagaaaaat tctgaagatg tagtaaaggc atatgtagaa tccagcgaac ctgttgttca
    18721 agcagaccaa agagcaaaag atatagttga tagagctgag agaatagcca atgagataag
    18781 aattgggtca attgaatatg cagaggatgt gctttctagt gttgagtata atttaagaga
    18841 aatattagaa gaagtaaaaa gagataaagc tgaacttaga cctaaaaaat aaattttaaa
    18901 taaataaaaa tggagctatc acacaaaaaa ttccaattta tgtgtgatag ctctctttta
    18961 atatagcaat tttttgttat ataattgtgc tttttagatt acttatatct tattttgata
    19021 ttataatgta gtttaatctt ttatttgttg aaatcaactt atttatttgt ataaataata
    19081 taggttattt tcaaattttt ttaataattt taccactgtc ttcttttaga ttagagttag
    19141 actgtgcaaa attataaaat aatactaaat catgtgcaat tatttggtat ggcactacat
    19201 gtagaaaata tagataataa aattaaggag gtagtatgaa ggaaagggct aaaaaaatat
    19261 tagattttat tattaaaaat aacaatgtaa catcacaaga actacaagaa aagtttaatg
    19321 tttcaaagag aactatttat tacgatattc tagctataaa taaacaacta ggaaaaagtg
    19381 gtaatataaa aaatgtaaaa cacaaattta tatttgaagg taatttatgt gatgcaagga
    19441 aaataatttc aactgaagaa gataaatttt tagatagtga ttacagaaaa acttttatat
    19501 taaacaagat attacttggc gaaaaaatat caatagaaaa actaacaaat gaaatgttac
    19561 tttcaaaaaa cacagtagta caaacaataa ctgatgtaaa aaaatacctt caaacaatgg
    19621 gattaagatt agagtataag ggaaagtaca aaattatagg tgatgaatac gttattagag
    19681 agctgtttct gataattgtt caagaaaacg tactggaaat taattctata agtgaggaag
    19741 taagtagttt cgatacaaaa ggacatataa aacttactga ttattcttta ttaaatctaa
    19801 ctaagtttgt ggaatttcta aataaaagga taagagatgg gaaaacatta tactcatata
    19861 aatatctaaa tgaagctaaa aaaattagtt actttagtaa ttgtaaagaa cttttatgtg
    19921 aagaagctaa tgaaaatgaa caagcctata tatgcactta cataagctca cttcccagct
    19981 taaatagtga agttaaggaa gatgttgttg aagaatatgt agataaactt atagataagt
    20041 ttgaagtaaa tacagcaata aaattagaaa gtaagcatga gtttaagaaa aatattttaa
    20101 ggcacctcca ctcttcttac aacagaatac gatttaagtt tcctattaga aatccaatgt
    20161 tagatgagac taagtataaa catgagtcac tttataaaat tataaaatca attatagaga
    20221 atgaagaaga gtttcctgta tttgagggga taagagaaga agaaatagga tttatagcag
    20281 cgtattttgg aggatattta agagggtcta gggataatgg tctaagaaga aataaagtct
    20341 tactagtttg tcctaatggt cttatggtat caaagagttt ggaaatacaa ctatataaat
    20401 acataccaac catagaaata gtaggtatag tatccattaa gcaactaaaa gaagtaaatg
    20461 tatactacga ttacataatt acaactatag atatacaaaa tgtaaacaac gtaattgttg
    20521 taaatcctct tttaacatca tcagatgttc aattacttat gaacaaacta ataagtgtaa
    20581 aagaaaacga gaagtatttc aatcttgaat taataataca agctataaga aaaaatggag
    20641 ttatcaataa tgaagaagca ctaaaagctg atttattaaa cataatacac aagatagatg
    20701 aaggagagat gtatcaacca atgttaaagg aattaattaa tgcagaaagg gtcaatataa
    20761 taaaaaatgt tagagattgg aaagaagcaa taaaaatagc ctcaaagcca ttgttagaag
    20821 ataactctat agaagagtta tatatagaaa atatgataaa gtcagttgag aaatatggac
    20881 catatattgt tttagctgat agatttgctc taccacatgc aagttctaaa gaaggagtaa
    20941 acaagcttgc tatgtcttta ctaatagtag aagatgaagt tgatttatta ggaaaaccag
    21001 taaatatatt tatggtatta gctgctgtag ataacactac tcacattaga gcattagcat
    21061 cactatcaga gatgatgtac gaggaagaaa atgtaaaact aataattaat ggtgataaaa
    21121 gttcaatact agaacttata aataagcaaa attaggagga atgaaaaatg aagatattag
    21181 cagtatgtgg ttttggtgta ggttcatcaa tggtattaaa aatgacaata caaaaggctc
    21241 taaagcagtt aggtatagaa tgtgaagtag aaaatacaga tataaattca gctagaggtg
    21301 ctgattgtga ctgtatattc acaagtcaag aattgttaaa tgaattaaaa ggaacttgta
    21361 atgtagatgt atatccagtt aaaaagtaca tggatttagc agaagtcaca gaagttgttc
    21421 aaactttttt aagtgataaa aatgataaga aatagtataa gttttaaaaa gtaaatttaa
    21481 gttaatcaga ataaaataaa aacttactaa gttcaaaaat agtaagaaag agaggaggga
    21541 tttagtggta gaaaacttaa acagttgact tatcactaaa aaatataatg cttgatttta
    21601 tttcaaataa tattttacgt aatccatcta tgttaattgg attaatagca gcatttggtc
    21661 ttgtactaca gaagaagaaa gcatctgatg ttataaaggg gtcactactt gcagctttcg
    21721 gaattataat tatggagact ggaacaggta tgttagttag ttcaatagca ccaattaatg
    21781 gagctttcca aagtataagt ggtggtgcag taacaaaagg tttaagtgat gttactttta
    21841 ctgcaagtta tggtggaatt ataggtttag caatgtttgc gggtttagct attcacctaa
    21901 tgatagcaag atttactcct attaagacaa tcttcttaac aggtcacatg ctttattggt
    21961 tcccattcat atttgtagct tcaggagttg aagctggatt aaatggtgga gtattaatag
    22021 cttttgcagc agtattatca gcactctatt ggtcaataat gccttggtta ttaagaaaat
    22081 atgtgtttga tgttatagga gatgattctt ttacattagg tcatccatct ggttgtttag
    22141 cacttatatc aggatttgtt gctaaattag taggaaataa agaaaaatct acagaagata
    22201 taaacatacc tcaaagttta tcatttttta gagaaatatc tataagtggt ggaatggcag
    22261 tgtttttaat gtttatagtt gttgggatag taataccagg tgcgtttaaa gatagtaatg
    22321 agtttattgg aacgtatgca ataaagcaag gtctttcttt tggagcaggt cttataataa
    22381 tgcttcatgg ggtaagaatg ctaataaatc aaataatgcc agcatttcaa ggtatatctg
    22441 aaaaagttgt gccaaatgca ctacctgctt ttgactgtcc aatattattt aattataaac
    22501 caaatgctgt attaatagga ttcttagttg ctatgctaac atctacagct atgattttaa
    22561 tatgtaacta ctttaatgta tttaatttgc tacttatacc acttgttata acttcattct
    22621 ttgagtgtgg agcagcagca gtaataggag aatcacaagg tggtatacgt ggagctataa
    22681 taggtacagc tatagctgca atatttatgg tagttttagt aggaatatct gctgtaattt
    22741 actcaggaac tattcaaaat tggatactaa tatttggagg taatgacctt tcattatttg
    22801 gtataatcag taggtttata ggttcactat ttggaggaat attatagata tgaaatttaa
    22861 atacatagat gagataagaa gattaattga tataatagaa aacgaagaaa aagtcaatat
    22921 ggaaaaagca gtacaaatcc ttgttgaagc aatagaaagt aaaaatgcta tattttcatt
    22981 tggagcttca catgcaggaa tattaacaga agaattattc tatagagcag gtggctgtgt
    23041 tcttataaat ccagtatttg caaaatctct aatgttagat acagcaccta taactcacac
    23101 aagtaagatg gaaagattag aaggatatgg tacagctcta gctgataaaa ctccaattaa
    23161 aaaaggagat gtagttatag ttcattcagt atctggtaga aatccagtaa gtatagagtt
    23221 tgctatgaaa gctaaggaaa ataaggcaat agtaatatgt ataactaatt taagttactc
    23281 aaaaagtgta tcttcaagac attcttcagg aaaaaaactt tatgaagtat gcgatttagt
    23341 gatagataat catggtcaaa agggagatgc ctgtgtagag atagatggat taaaccaaaa
    23401 agttagtcca acttctactg taatagcaac tactgtgatg aactctatta tagctcaagc
    23461 tactcaggaa ttagttaata aagggctaaa gaatcctccg attttctata gtgccaatat
    23521 tgatggtgga gatgagttaa ataagaagat ttttgatgaa tataaaagtg taattcatta
    23581 tgaatattaa ggttaaaaaa atatgttaat gaactttgaa gtttatcaac aaagaatatt
    23641 aataatatat aataaatgct atcatttaaa agctgtaggt aaaaatctac agctttttat
    23701 tattgtagat taaaaagatg cgtaattaaa ttttaaattg gagaaaataa attggaaggt
    23761 atcaattata aatattagaa acgttgtcat agaaaaaaac tatattaaaa aaaagtacat
    23821 agaaaaaact gattagacaa taaatacatg ctttgataat attttaaatg gagtgaaaat
    23881 gtgtagaatt ataatgaaaa aagaaaataa aaatgaaaaa agaaaataaa atggagggaa
    23941 taaaagtggg tatgttaaaa aaattatgtt cactagctat ggtagcaatt atgactacaa
    24001 cacttataac agggtgttca agtggaaatg gaaaagattc aaaaaaggat ggcgaaaaag
    24061 aaaaactagt tctttggatg ccaccagtag aaaataatat gaaggaagtt tgggacccta
    24121 ttttagataa atttgaggaa aaaaataact gtgaagtaga tttacagata attccttggg
    24181 ataattattc tgaaaaattt gcaacagcta taagtgctgg cgaaggtcca gatgtaggat
    24241 atatgtatgc agaaatgtat ccacagttta tagaaagtgg agcagtagaa gatttgacta
    24301 actatgcaac taaagaggat aaagagcaat ctatttatat aaaaacatct gaaatgatgg
    24361 gtggaatgta tggtatgcca ttccaagcag caaatccagg ggtattatac tataacaagg
    24421 atattttaga gaaattagga gaaaaacctc ctaaaacatg ggaagatttt aaaagaattt
    24481 gtaaaaaagc aacgcaagat actgatggtg atggaaagat agaccaatgg ggattagctc
    24541 agggatgggg agctaaaact tttggtaact taaactggaa ctggtatcct tatttatggc
    24601 aagctggtgg agatatattt aatgatgatt taaaatcggt taaatttaat gataagagtg
    24661 gtttagaagc agctaagttc ttaaaagaat tacaagcata tgtgcctgaa gattctttat
    24721 ctaaagatag taatgaaatg atagaaagtg tatttggacc agggaaagca gcatttacaa
    24781 taatgttatc ttctgctgca acaagtgtat ttgatgaatc attcccggat ttaaattggg
    24841 gatttgtaac tggactagaa gataagaaag cagcaacttt tggagcagta gaccatttat
    24901 ctttaatgtc atctgcgaaa aataaagaac ttgcatacaa gttaatacaa catatgttaa
    24961 gtgttgaatc aatgacagaa tttcataaag caataccaag agcaccaata acaaaaggtg
    25021 aaccatatca aggtgatgaa agattcaaag aaatggtgga aaatgacaaa aatgtatata
    25081 gaccattagt agtaggacct catggagttg aaatatatga atatttatgg aaagaacttc
    25141 aaactatgat ttcaggagat aaaactccaa aacaagcgtt agatgatgct gcaaaatatt
    25201 caaatgattt attagcacaa taaaagttta aagctgtaat atgttgtaaa ggactttaca
    25261 gattaaaatt agcattgtat ttataaatac aatgctaata tttatattgg agaggataaa
    25321 atggaaaact tagttagaga gtttgaagaa aattcaaaaa gaaaaaaaac aaaattaaat
    25381 attaaaccat atatgtatat attgccattg ggaataattt tagtggcgtt ttatgtgata
    25441 ccaataataa tgtcaatata ctttagtttt acaaagtata atattattag tccagctact
    25501 tttataggtt tagaaaatta taaaaaatta tttacagatg aaacattaaa agtatctatt
    25561 ataaatacta ttaaatttac tgttgttgta gtaccttgcc aaactatatt atcattgatt
    25621 ttggctgttt ggattacagg aaaaggaaat agtaaaatag cttcatttgc taaaggtgct
    25681 atatttatac cagtattatc atctatggtt ttgataggta tggtatggag ggctttattg
    25741 aatggagaag gttctattat atatcaggta ttaggtgcct ttggaataga atcatctaaa
    25801 cttttaggag atagtaagac agcattacct acacttatgt ttatatctat gtggaaaaat
    25861 atagggtatt tcatggtaat ttatatttca gctataatga atttaccaaa acactgctat
    25921 gaagtagcga aggttgatgg ggctacaaaa cttcaagaat ttataaaaat aactgtacct
    25981 ttattaaaac caactacaat aatggttgta tttttagggt cgatatggtc attacaagta
    26041 tttgacttaa tttatactgt gacaggtgga ggtccaggta tatctactat gagtatagtt
    26101 atgcatgcat ttaacttaaa ctttaaaaac tttaattctg gatatgcaat gactgtagca
    26161 aacgtattat ttttgttaat agcagtagtt tcgattttac aaaataagct tgtaagacga
    26221 gacaattcag atttttagaa aggtaggtat aatatgaaaa cagcaagaag attattaagt
    26281 gatggtttac ttttatttat agcgtgtgca agtttggtac cttttatata catgttgata
    26341 atatcattga agataacata taactcatat agtttggata tatctttttc aacagtaacc
    26401 cttcaaaact atatagatat ttttactaaa aaaggatttg cacaatattt ttttaataca
    26461 gcaatagtat ctttttcagg tgtattatta aatttagtat ttagtacatt agcaggatat
    26521 tcttttgcga agatggattt taaaggtagt gataagttat ttttatttat gataatgact
    26581 ttaattatac cttcccaagt tacaatgata ccactatata taataatgaa acacttagga
    26641 tggataaata gttatcttgc acttattatg ccaataccaa ctgcatttgg cgtatttata
    26701 atgagacaag ctattttagg agtaccaaaa gagttattag aatcagcaaa gatagatggt
    26761 tgctcggatt ttagaatttt aatacaaata gtgttgcctt taattaaacc agcattaatt
    26821 acactagcaa tatttacttt tatgggagca tggaacgagt ttatgtggcc tcttatagct
    26881 acaactaaag atgcaatgag aacattaaca gttggattat ctactcttaa gacattccag
    26941 ataactaatt atggacagat gatggcaggg gctacaataa catttttacc gccatttatt
    27001 ttctatttga ttttgcagag taaatttgtt gaaggtgtgt ctttgtctgg tataaaaggt
    27061 taaatagtct aatttaataa tatataaaga aacttatata aatatggagg aatttaaact
    27121 atggaaattt catatgtact gaaagattgc acattcaaac acaataaata ttcagttata
    27181 gaagctgaaa cttggaaaga taaggatttg gatattgtag tagctaaagg tgaaaaattt
    27241 gcatttcaaa ttatgttaaa agcaacagaa gaatttaatt gtacaattga taaaagcagt
    27301 gctatatcat ggaaaggcct aggaaataga gtaaggttag cattaaatgt gcctaatagt
    27361 ttagaaaata attttagtat aaatatatta ggatatgttc aagatgatac aaaggcattt
    27421 gtgaatgatg ctattttaag ggataaagat gtgttagtag agcaatattt gcctcaaaca
    27481 ttttggattg aaggtcaagt accagaagat tttgaagaaa ataaccttga tataggtatt
    27541 gatatattta aaagctttgg atatgaagac gaagaaaaag tttgtacaat tgatgttcct
    27601 gtaaaagtaa gaaatgttgt gctaaagcct ttagatgaaa gcaagttctt tttagattta
    27661 tggcaacatc caagttgttt agctagaatg tataaagttg agttatggtc tgatatacat
    27721 tttgaaatag tcgataacta tttaaaagaa ttagcatctt taggagaaaa agttgcaaca
    27781 gttatagttt cagattatcc atgggcaggg caaagttgtt ataaagttta taaaaatcca
    27841 tcaaacttat atgaatataa catggtaagt gtatcaaaag gattggatgg aaagattaaa
    27901 tgtaactttg agagtatgga taggtatata ggcatagctg ataagtataa aatggctaag
    27961 gagatagatt tatttggtct attaggtaat tggtgtgctg gtgagtttgg aaatcctgta
    28021 gaaggttata aggaccctat aagagtaaga tattttgatg aaaaggataa agtatttaaa
    28081 tttataaaca atacaaatga tttaaaagaa tatataggat tagtattaaa tcatttaata
    28141 gagtgtggat tatgggacag agtaaggatt atagctgatg aaccaaataa tccagaagta
    28201 gtcaaagagt gtatagaatt tataaattct actgtaggta ctcatcaagt taaatataaa
    28261 tcagcaactc acgaccaaaa ctttttagac agagctaaag atgaaattga tgatatgtca
    28321 ataaatttaa aacttactat acaaaattat aaagatatag aaagtcttaa gaaaaaaatc
    28381 aatgacaagg gtgggatatt gacttggttt gtatgctgtt ttccagagaa accaaatagt
    28441 tttttaagtt ctccttttgt agaaaataga ataataggat ggtatactta ttactttggt
    28501 cttgatggat ttttaagatg ggactataat ttatggacag aagacccttg gaaagattca
    28561 agttacaaat tccctatttg gaaagctgga gatatgttct ttgtttatcc tggaaaagat
    28621 ttaaagccag ttagaagtgt cagaatggag aatttaagat ttggaataca ggactttgag
    28681 ttatttacaa tgttagaaaa agaaaaggga agagagtata tagaagttga gcttatgcaa
    28741 gaattattaa ataaaaaaga aaatgctgaa attaaaggtt ttggtgacat agaactagga
    28801 tattcacttg ataattgggg atatgataaa gttaaaaaat atgtattaga gttgttagac
    28861 agagtataaa aaatataaga gtgtctcaaa atgaactttt tagttcatga ggcactcttt
    28921 ttgtatgaaa tcaatatagt ttcattgttt caacaatgaa aaataggatt atttctattc
    28981 taagacagcc ctttagtttt ttagttgtat tcaatgtttt aaaactaact ataaacgcta
    29041 taaagtttat aaatgacaat aattatatat aacatgatgt actttaattt gctataatag
    29101 ggattgaata atattatatg gaagataaca ttgtttgggg ggattaatat aaaaagaata
    29161 ggtattatta taatgttttt tttaagtata tcttttattg gattttatct tgtaataaga
    29221 gataacagtg atacagttaa gatgcaacga gggataaata taggtaatgc actagaatca
    29281 cctaaggatt ttccgtggga tgtaaaaatg tcaaataagt tttttgatga tataaaagat
    29341 gcaggttttg acacagtacg tataccagta aggttttctg attatacgtc agattctgat
    29401 aattttaaaa ttgatgaaga attttttaaa aagattgata aatatgtaga ttatgcattg
    29461 gataaagact taatagttgt gttggattta caccattttg aagaaattat gaaagaacca
    29521 agagttcata aagaaaagtt tttaaaaata tggcaacaga tagctaatag gtatcaaaaa
    29581 tatgataaaa aattggtatt tgaattatta aatgaaccta aagaaaattt atattcacag
    29641 ttactaaacg aatacattga agaagcaata aagataatta gaaaaactaa ccctaaaaga
    29701 actattatag taggaccata taatttctat caaattgatt atttgaatga attaaatatt
    29761 cctaaagatt ctaatatagt agtttccttt cactattacg agccaaatga ttttgcattt
    29821 caaggaaata tctatcataa gggatttgag cacttaagca atattacttg ggaaggaaca
    29881 aatgaacaaa tggattattt aaaaaaaagg tttgatactg tggaaaattg ggcaaataaa
    29941 aataaggtaa aaatcttctt gggtgaattt ggagtaacaa aagaagcacc agaaacttca
    30001 aggagagctt gggtaaaagc tgtaagagaa gaagctgaaa aacgaaactt ttcatgggct
    30061 tattgggagc ttgcttctgg atttggtata tacaatcaaa ttgaagggac ttgggataga
    30121 gaaatattaa gtgcattaat agaaaagagg tgattttatg aagaaaaatt caaaaaagat
    30181 acttataact ttaactatta ttactaatat agtatatata ttgtggagaa ttttttatac
    30241 agtacctaaa gaagaaggga tgtttgccct aatctgtgcc ataatacttc tttttgtaga
    30301 gataatgggt atgatggaaa tgtttgtgca ttattatggt atgtcaaata ttgagtaccc
    30361 tgaaaaacca atcataagtg aagaacttta tccacatgta gatgtattca ttgcaacata
    30421 taatgaatct gttgatttgg tgagaaaaac tgtaaatggg tgtattcata tgcagtatcc
    30481 agataagaaa aaggtacata tatacatatg tgatgatgga aatcgtgaag aaatgtgtat
    30541 tctagcagaa aaaatgggtg taaattacat aactagaact gaaagagaag gagcaaaagc
    30601 tggaaattta aataatgcaa tgcaacatac aaattcccct ttgattgcta catttgatgc
    30661 agatatgata ccaatgcatg actttttaat tgctacagta ccatactttt tgaaaaatga
    30721 gcaagctaaa aaagatggag aaaaagagga gtatgaaaag gttggttttg tgcaaactcc
    30781 acagagtttt tataatccag acttgtttca atttaatctg cactctgaag gaaggattcc
    30841 aaatgagcaa gattattttt acagagacat tcaattggct agaaatagga caaattctgt
    30901 aatttatggt ggtagtaata cagttatttc aagggaagca cttgaagaag ttgatggatt
    30961 ttatacttat tctattacag aagattttgc aacaggtatc ttaattcaaa gtaaggggta
    31021 tagatgttat gctattccag aagtacatgc atcaggttta tctccgacag atttaaaaag
    31081 tcttattaag caacgtgaaa gatgggcacg tggatgtata caaactggta gacgtctaaa
    31141 tattttgttt aggagaggtt taggtttttg gcagaaaata agttatattt cttcaattac
    31201 ttattggtat gcaagtataa aacgctttgt atatataatg gcacctattt tattttctgt
    31261 gtttaatgtg attgtagtaa aatgtacgtt acttcaagta ttggtatttt ggttgccaat
    31321 gtatatttta agtagtttat ctcttaaaat attttctcaa aatataagaa atactagatg
    31381 gaccaatata tatgaaacta taatgtttca gtctttaatg ccagcagtaa tacttgaaac
    31441 gtttgcaata tctaaaaata aattttcagt aacaaataag agtaaattag aagaaaatag
    31501 aatgtataag tttttacagg gaatacctta ttttatttac atggtcttat ctatcatagg
    31561 aattttaaaa atgtttgtag caatatttaa aatgagttca atgacatatt cagttgtatt
    31621 attttggttg attggaaacc tttttaacct tgttatggct acattattta tatcagggag
    31681 acagcagtta agaaagtctg agcgttatat tgctgaaatt gattttaagt taaagcaaaa
    31741 ttcttatgtt ctttcttcaa aaacaattga tatatcagaa aatgggtttg catttttgct
    31801 agaaaatcca gaatatattt ccccagaaga agagtttgag gtagagttta gagaaaaatc
    31861 tggaaatgaa atgtatatag ctaatatgaa agcaaagatt gttaatgttg tggaagtaaa
    31921 ttcaaagtgg aaatatgcag cttatattac acatattgag gatagtgaaa tagataattg
    31981 gatgtgtatt gttcacgata gaatacctac tttaccaatg acaatatcta atcaactagg
    32041 tttctttgat gatttgcaaa ttaatgtaaa aaaacgtatt gaaaaaacta gaacattatc
    32101 acgaagaagt ccaagaatca atatgaattt tcagatggat ataaaaaata caggtaaatt
    32161 aagaatagtg aattttaatt atcaatatgt attattaaat tttgagaata aaagtattta
    32221 tcctaaagaa atagcattgg aaattaatga aaatattgtg ttagaatgtg atttgtgtga
    32281 gggtaaaatt gatgaaagag gtatcttata taaagtaaat aacattgaca gcataatgca
    32341 aaatttcttt ttaagagatg aaatgatgga ttggatatta caaaataaga caattcttgt
    32401 ttcaaagcct agtgaaaaga aggaaaaaag tatagatgag tttgaaccca tggaatatat
    32461 ttaggagaga tgaaatgaag actttaaaag tgtttttttg ttctacaaaa tatcttattt
    32521 tggtgtttat tttttttata aatactactt ttataagttc agcaacaagt gataaaattt
    32581 tagatttatc ctttaaaaag atagagactg acttaagttc taagattaca tatgaggata
    32641 caggtgtaag aatagaaacg gatagttcta aaagcgataa ggaacgttat ttatatattt
    32701 accaaaatat aaaagaaaat tggagcatgt ataataattt ttatattgaa attcaaaata
    32761 aaaataaaag ttctcagaga ataaatttaa gcatacaaag taaaaatatg cttgaattta
    32821 gattaaaaga aggtagtgaa gtatttttag aaggtaaaaa tataatatat tcagataaaa
    32881 taaaagaagg atgcatagaa gtaccaggtg aatttgaagg caaaatttat gtgaacttta
    32941 atagccttat aaatgaggaa agtaatgttg tgctggactc aaatatgctt tcaaacattg
    33001 ttagttgggg aattacgttt attccatctg atgaagaaca taatattgtt attataaaaa
    33061 agatatcttt attgtctgaa gagaaattaa gatttttaaa taatattaaa ataattggcg
    33121 atgaagaagt acaaatacca gtattgggtc aaagtataag tcagtatgaa gtattaggtc
    33181 ttaaaagtga ttctaaaatt aagtactctc ttatgggaaa acaagataat gtgagcatat
    33241 ctcaaaaagg taaattaaca ttaagtaata aagccaaacc tggacaaatt attttacaag
    33301 ttaatgttga tgataagttt aaaattggaa agaaaataac attaactgag tcttggagtg
    33361 ttaataaaaa agataaggat ggtgttccat atacattagt atatcctgag caatcaccaa
    33421 cagtacaaga tatgaaaaaa ataaacttta tgaataacat cataacgttt gttcgaattt
    33481 tatttgtatc tctagtaata atatgctttg gaatatattt atattggaaa aaatgttcaa
    33541 aaactaagta aagagaggag gttagattgt gttattttca agtttaattt ttttatttta
    33601 ttttttacct ataactttgg ttttatatta tgtatttagg tttaaccgaa cgatacagaa
    33661 catgatattg cttgctgtta gtttattttt ttatgcttgg ggagagccaa aatttgttgt
    33721 tatcatgata gtttccataa taatgaacta tatatttggt ttactggtag atagatatag
    33781 ggaaagtaaa ataaaagtaa aagttttttt agttttgatg tgtgcataca atataggtgt
    33841 tttatttata tttaaatatc tagcttttgc gctaagaaat attagtgaac ttattagtac
    33901 tgaattgact atacctagta ttgttttacc cataggaatt tcatttttta catttcaggg
    33961 aatgtcttat gttatagatg tatatcgtag acatggagaa gtccaaaaga acccttttta
    34021 tgtgggattg tatatagcat ttttcccaca attaatagca ggtcctatag ttagatatga
    34081 gagtgtggca gaacaaattc tcaatagaaa agaaacttgg aataaatttt ctattggtac
    34141 ttgtaggttt ataacaggtc taggtaaaaa agtattaatt tcaaataaca tggctatagt
    34201 agcagactat atttacacta tgaatagtca aggagaaata gctgcatcac ttgcatggct
    34261 tggttcaata gcctatactc tgcaaatatt ctttgatttt tctgcttatt cagatatggc
    34321 tataggctta ggtcttatgt ttggttttaa atttgaagag aactttaatt atccatatat
    34381 atcaaaatca attagtgagt tttggagaag atggcacatt tctttgggaa tgtggtttaa
    34441 gagctatata tactttccgc ttggaggttc tagagtagca aataaagata ttatgattag
    34501 aaatatgttt atagtgtggt tattcactgg aatatggcat ggagcagaat ggacatttgt
    34561 aatttggggg attttaaact ttgttttcct aattatagag cgatttataa tgtttgaaaa
    34621 gattgaaaat cacaatttta taaaacatat ttatgcttta ttagtagtta attttggatg
    34681 ggtattgttt aggtcaccta atttaaaaga agcatataat tattttaaag ctatgtttgg
    34741 aggcaatgga gctatatgga gtgattatac atatatgttt ttaaaagaat actgggtttt
    34801 ctttatattt gcatttattt ttagtgtacc tattgcaaag aaaatcaata agtttgttgt
    34861 tgaacaagct aaatatagta tgatatttaa cgtattttat ccaatctcag ctattttatt
    34921 gttttttatt tctgtgacat atttagtaac tggaagctat aatccattta tttattttaa
    34981 tttttagttg ggaggaaaag tatgaataga ttttttaaag taagattaat aactgcaatt
    35041 atgttcttaa ttattttatt cgctttctct atctttaatg tttgttattc tggaaaagat
    35101 acgatacagg aaataaaaaa ttcaatagct gaaaaaagta gtttaaaaga tacaatatca
    35161 tctattgata atacagtgaa tgacaaagta atatacaaaa atacgttcat tgaaacttat
    35221 ggctatttac aaaaattaat gggtaaaaat gagttttcaa aatttagtgt tgtaaaagat
    35281 acttctggaa agatgcatta tacatatttt gctaaagctc caaatccagt tggtgtgata
    35341 gcagataggg taagaaaatt ttctgatgaa atggaaaagc aaaacactaa attggtttac
    35401 ctaatgactc cagataagta tataaaagga gtgacaaaat ttcccaaagg aataccttat
    35461 tcttatagta atgagacagc agataatttt ttaaatcaac taaaaaagta taatgtggac
    35521 tatattgatt ttagagaaaa tattttaaag agtggtattc caaaagatga tttatttttt
    35581 aaaacagacc atcactggaa agtagaaact tctttttggg catttggaga attagttgag
    35641 caacttaata aaaaatacaa tatgaattta gatgaaaatc attattatag agataaggac
    35701 aattataatt caattgtata tcctaagtca tttcttggtt ctatgggaag aaagactggt
    35761 atcttatatg gtggtataga tgattttaca ttaatttatc ctaaatttaa gactaattat
    35821 acttattata cagattctaa atctcaaaaa tttgaattaa aaggaaggtt tgaagagagt
    35881 ttaatattat catatccttt taatgcagac cttaatttaa tggatggtca atcagataag
    35941 tattttacat atttattggg gaacagacca ttagttaaaa taaaaaatat agaaaatcca
    36001 gatggattaa aagtgctgtt tgttaaagat tctctaattg taccaacagc agcatttttt
    36061 tcctctgttt gttcatctat agatatgata gaccctagat attatgatgg agatatacta
    36121 gaatatgcaa aatcccatga ctatgacttt gtttttatat ctgtatatcc ccaaaatctt
    36181 acaaaagaat tttttccgtt ttgtgagtaa atttctaaaa aagttttctg ataaaataaa
    36241 tggctaactg gtaaaaagtt agccatttat tttgagtgtt attctaagaa atattttttt
    36301 tactaaatac ttctttatct atttttccaa gtgattttcc tacagtaatt gatgatacca
    36361 tagcaccatt aacatttatc atagtacgtc ccatatctag tatagggtct attgcaagta
    36421 taccaccaac taatggaaaa taagaaccaa gaccaactcc agagataaca actgaaacgg
    36481 ccattgtagc agttcctgga agaccagcta ttccaaacga actaattgtt ataacaatta
    36541 acaacataac ataaaagctc atatccatgt ttactccagc catattggca agtgttacag
    36601 ccattaaagc aggatatata cctgcacaac cattcatacc catatttgca cctaaacttg
    36661 ctgtaaagct tgatacaccc tcatcaactc cgtgattttg tactagactt tctatcgtta
    36721 caggcaatgt tcctaaacta gaacgtgaag taaatgccag aagtaaagct tcagttgaat
    36781 tttttatata agtaatggga tttagaccat ttaaagttat aaaaattaaa tgtatgataa
    36841 ataatattgc aactgatata taaagagcta atataaactg taaaacagat actattgaac
    36901 taataccttg tgaagttatt gtatttgcaa gcagagcaac cacagcataa ggcataaatt
    36961 taataacagt catagacaca cttacaatta ttttgtaaga tgcttctata aaatctataa
    37021 atggttttat tgtctcaaaa tgttttttac ttaatctctt tattgaggta gctataaatg
    37081 tagcaaatat tattgtagct acaatatttc catttgccat agccattaca ggattagatg
    37141 gtagtaatcc tcttaaagta tccacaagag agtttatttc tttaagttct tgattttcaa
    37201 ctaaagtagg catattagct cctacaccta acttcattat atttgcaacg ataattccaa
    37261 caattgcagc tatagcagta gtagcaagaa gcatacctaa agatttaaat gtcagagttc
    37321 caagattgtt atctttcata tttataatta cttttataat ggataagaaa actaaaggta
    37381 ctactaacat ttttaataag tccataaatc cagaaccaaa taggctatac cacttactta
    37441 cttcttgtag ccaaatgact tgagttgtat cattagggaa accagcaaaa gcttgtatta
    37501 tgattcccaa tatgagtcct agtactgtag catatattgt tctcaatgaa aattttattt
    37561 ttttcttttc aaattgtcta acaataaaga acgtacctag aagtaatatt atgaaaaaaa
    37621 ttgttttaaa atctgtaatc ataagaaatt gtgataaaaa tgaactttcc aatatttttc
    37681 tccttttatt taaaaataaa tttttctaag tttaaatgat atca
//
LOCUS       DAGSWI010000026        35791 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.026, whole genome shotgun sequence.
ACCESSION   DAGSWI010000026 DAGSWI010000000
VERSION     DAGSWI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35791
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.026"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..897
                     /locus_tag="MJF80_001180"
     CDS             <1..897
                     /locus_tag="MJF80_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBZ0279852.1"
                     /translation="DFKTFLGRYNHSIRFINEELNYESIHILENIIEEEPELIEPYVI
                     LSLLYDKLGNVKKREGYLDKLKELDKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILP
                     YIIVGCVLIGMGIYLIQSKKRIENLNNQISSKEEKLSETDKKLGETSKKLEKTNKELN
                     EVKNKEPEREITVADEEDLYYQALNLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQ
                     LAITNKLLGNKDEAIKYYKKYINTYTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYS
                     LRSEVPDSMYNNSKIKEILSEK"
     gene            1079..1450
                     /locus_tag="MJF80_001181"
     CDS             1079..1450
                     /locus_tag="MJF80_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBZ0279853.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            1443..2132
                     /locus_tag="MJF80_001182"
     CDS             1443..2132
                     /locus_tag="MJF80_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBZ0279854.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            2652..3518
                     /locus_tag="MJF80_001183"
     CDS             2652..3518
                     /locus_tag="MJF80_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBZ0279855.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            3760..4569
                     /locus_tag="MJF80_001184"
     CDS             3760..4569
                     /locus_tag="MJF80_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279856.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            4713..5777
                     /locus_tag="MJF80_001185"
     CDS             4713..5777
                     /locus_tag="MJF80_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBZ0279857.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGAIFGGSYKTYYTQNFYK"
     gene            5805..6524
                     /locus_tag="MJF80_001186"
     CDS             5805..6524
                     /locus_tag="MJF80_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBZ0279858.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            6548..7132
                     /locus_tag="MJF80_001187"
     CDS             6548..7132
                     /locus_tag="MJF80_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBZ0279859.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            7134..8483
                     /gene="pabB"
                     /locus_tag="MJF80_001188"
     CDS             7134..8483
                     /gene="pabB"
                     /locus_tag="MJF80_001188"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBZ0279860.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            8473..9216
                     /locus_tag="MJF80_001189"
     CDS             8473..9216
                     /locus_tag="MJF80_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV family protein"
                     /protein_id="HBZ0279861.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDISYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEDVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            9250..9951
                     /locus_tag="MJF80_001190"
     CDS             9250..9951
                     /locus_tag="MJF80_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279862.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            9977..10549
                     /gene="folE"
                     /locus_tag="MJF80_001191"
     CDS             9977..10549
                     /gene="folE"
                     /locus_tag="MJF80_001191"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBZ0279863.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            10613..11404
                     /gene="folP"
                     /locus_tag="MJF80_001192"
     CDS             10613..11404
                     /gene="folP"
                     /locus_tag="MJF80_001192"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBZ0279864.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            11415..11780
                     /gene="folB"
                     /locus_tag="MJF80_001193"
     CDS             11415..11780
                     /gene="folB"
                     /locus_tag="MJF80_001193"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBZ0279865.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            11773..12279
                     /gene="folK"
                     /locus_tag="MJF80_001194"
     CDS             11773..12279
                     /gene="folK"
                     /locus_tag="MJF80_001194"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBZ0279866.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEKLIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            12260..13045
                     /gene="aroF"
                     /locus_tag="MJF80_001195"
     CDS             12260..13045
                     /gene="aroF"
                     /locus_tag="MJF80_001195"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBZ0279867.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKRQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            13300..15090
                     /locus_tag="MJF80_001196"
     CDS             13300..15090
                     /locus_tag="MJF80_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBZ0279868.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            15108..16274
                     /gene="rpoD"
                     /locus_tag="MJF80_001197"
     CDS             15108..16274
                     /gene="rpoD"
                     /locus_tag="MJF80_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBZ0279869.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            16439..17128
                     /locus_tag="MJF80_001198"
     CDS             16439..17128
                     /locus_tag="MJF80_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBZ0279870.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            17142..18239
                     /locus_tag="MJF80_001199"
     CDS             17142..18239
                     /locus_tag="MJF80_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBZ0279871.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKFDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            18302..18874
                     /locus_tag="MJF80_001200"
     CDS             18302..18874
                     /locus_tag="MJF80_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBZ0279872.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(18934..19401)
                     /locus_tag="MJF80_001201"
     CDS             complement(18934..19401)
                     /locus_tag="MJF80_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBZ0279873.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(19787..20263)
                     /locus_tag="MJF80_001202"
     CDS             complement(19787..20263)
                     /locus_tag="MJF80_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBZ0279874.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(20383..21474)
                     /locus_tag="MJF80_001203"
     CDS             complement(20383..21474)
                     /locus_tag="MJF80_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBZ0279875.1"
                     /translation="MSKIECLFTIFPVLILMYTTFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKKFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(21467..21856)
                     /locus_tag="MJF80_001204"
     CDS             complement(21467..21856)
                     /locus_tag="MJF80_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0279876.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            22048..22494
                     /locus_tag="MJF80_001205"
     CDS             22048..22494
                     /locus_tag="MJF80_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279877.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            22758..23483
                     /locus_tag="MJF80_001206"
     CDS             22758..23483
                     /locus_tag="MJF80_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0279878.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            23470..24714
                     /locus_tag="MJF80_001207"
     CDS             23470..24714
                     /locus_tag="MJF80_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0279879.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLIIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGKGTEFHIIFHKMYDSD"
     gene            24794..25471
                     /locus_tag="MJF80_001208"
     CDS             24794..25471
                     /locus_tag="MJF80_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279880.1"
                     /translation="MEILRVENITKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            25472..28033
                     /locus_tag="MJF80_001209"
     CDS             25472..28033
                     /locus_tag="MJF80_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0279881.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRRNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLFTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            28254..29528
                     /locus_tag="MJF80_001210"
     CDS             28254..29528
                     /locus_tag="MJF80_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBZ0279882.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(29689..32730)
                     /locus_tag="MJF80_001211"
     CDS             complement(29689..32730)
                     /locus_tag="MJF80_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBZ0279883.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKEAWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDLANKKIEVNLPQNSSFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            32937..33236
                     /locus_tag="MJF80_001212"
     CDS             32937..33236
                     /locus_tag="MJF80_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBZ0279884.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(33474..35702)
                     /locus_tag="MJF80_001213"
     CDS             complement(33474..35702)
                     /locus_tag="MJF80_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBZ0279885.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
BASE COUNT        14198 a         3879 c         6019 g        11695 t
ORIGIN      
        1 gattttaaaa cttttttagg aagatacaat cattctataa gatttataaa tgaagaatta
       61 aattatgagt caatacatat tttagagaac ataattgaag aagaacctga actaatagag
      121 ccatatgtaa ttttatcttt gttatatgat aaactaggta atgtaaaaaa gagagaagga
      181 tatttagaca aacttaaaga gctagataaa gataatcctg tatttgaaaa aaatgaagaa
      241 gaaaaagaag atacaagtaa aaaggaagaa ataaaaaaag taaaaagaaa gaaaagtatt
      301 ttgccatata ttatagttgg ctgtgtttta ataggtatgg ggatatattt gattcaaagc
      361 aaaaagagaa tagaaaactt gaataaccaa ataagtagta aagaggaaaa gttaagtgaa
      421 actgataaaa agttaggaga aacgagtaaa aaattagaaa aaacgaataa ggaattaaat
      481 gaggttaaaa ataaagaacc agaaagagag ataactgtag ctgatgagga agatttatat
      541 tatcaagctc taaatttaaa gaaaaacaaa gaatatgaaa aagccataga taattttaaa
      601 agtgtagttt cttctggtaa aactaaaaaa tatatttctg agtcaatata tcaattagca
      661 ataacaaata aattattagg aaataaggat gaagctataa aatattacaa aaaatatata
      721 aatacttata ctaaaaatga ccagtattat gatgattcat attacgagtt gggtatgctt
      781 tattatgata atggcgattt aaaaaatgca caacagactt tttatagttt gagaagtgaa
      841 gttccagaca gtatgtataa caattcaaaa ataaaagaaa ttttaagtga aaaataggtt
      901 tatatagtta agttttttga aacaaccaag tatgtatttt attaaattaa aataaaaata
      961 aatttaaaaa gagattgtgt caaattataa ctttctattt taaagaaaga tatttgcttg
     1021 ttgataaaac tacaaatata tgatttgaag caatctcttt aatttttgga ggtggaaaat
     1081 gaaagttttc aatcaattgg taataatttt atctatctgg gtaataggag agtatataag
     1141 ttcctttata caaggaatta tacttattcc tgggagtata gttggtatgc tattattatt
     1201 tctactacta caatttaaag ttttagactt gagtagtata gaaaatgtaa gtggattttt
     1261 cctcgataat atggctatat tttttattcc agcaggtgta tcacttataa agtcattaga
     1321 cctaataagg gaaaatgtat tagtgctttt attagtcata tgtttaagta cactaatagt
     1381 aatgtatact actggaatta ttgtggagaa aatgataaaa aagaaggaag aaggacaaaa
     1441 aaatgtataa tgtattacag acaccagtat ttggtataat agtaacaata gtttttttca
     1501 atttaggaag atacattcaa cgaaaaacaa gtaatcctat atgtaaccct ttattaattg
     1561 caatcgtagg aataatattg tttttaagca tatctaaaat cccttatgag aattacaaga
     1621 taggagctga tagtctaaat ttctttttag gtcctgtcac agtagtactt gcagttccac
     1681 tttataaaca atttgaatta tttaaaaagc atatgtttga gattattgta ggaataggtt
     1741 gtggtatagt gatttctttt gtatctgtat tgataattgg caaaatagct aattcagatg
     1801 taagtataat aaattcacta attccaaaat ctataacaac accaatggga atatctttag
     1861 caaattcttt aaatggtata gaatcaataa cagttgtagc cataattttt acaggtattt
     1921 ttggtggtat ggttgcttct acagtattca aactggggaa tataaatcat ccagttgcta
     1981 aaggaattgc tcttggaaca tctgcacatg ctttaggaac tacaaaggct tttgaactgg
     2041 gagaaataga aggagctatg agtggtgttt ctattggagt ctctggtaca attacagtaa
     2101 tactaattcc aattataatg aattttatat aaaaaataaa attaagacga gtaccttgaa
     2161 tacttaaaac aaaataaaat aaaaaaaatt taaaataaaa actgaatgaa aaaaatgcaa
     2221 ataatgaaat aaaaaaatca tgtactgaaa ttataaaaag aaaaatttca tttaggaatt
     2281 aattataaag agacaattac taaacttgaa tttgctaaaa taaaataaat tactgcgaaa
     2341 atagatattt agaaaaaaat actataaatt tactatattg caagttaaat aataagcagt
     2401 atcatattat gctagaaaaa gtttcattta aggcataaat gagatgattt gaacgttttt
     2461 aaagattaga atataaaaga ttgcactaga catatatgaa aaagataata taatagatga
     2521 tgtaatagat gacagttcaa gaaattataa aaaaaattta tttgaaatat tatctattac
     2581 atcaggaata atatatgtaa gagtaagctt ttaaaattct aaagatttag aaagattgaa
     2641 gggagaaaga aatgagtgaa gttaagaata ccctacaaaa acatccagtt ttaagatatt
     2701 caacaatgct atttgggatg ttgatgacat ctataggaat aaatgggttc ttaagaccag
     2761 cacatctact tagtggaggt gctacaggta tagcaacatc tataaactat ttgacaaata
     2821 taaatgtagg tttattaaca tttttaatca atattccaat atttatctta gggttcatat
     2881 accttgaaaa agagttttgt atttcaagtt tggtaaatat gattgtattt tcattattat
     2941 taggagcaac tcaggaaata agtaatatta tacctataca tgatatattg cttcaaagtg
     3001 tatatggtgg tatattatct ggtttaggag tcggagttgt atttagaact aggtcttctc
     3061 aaggtggtac agatataata gctgcaatac taaaaattaa gaagaatata gaaatgaaag
     3121 acactgcttt agcaataaat ggcttgatag tacttacagg aagtttttta tttgggttgg
     3181 atttagcatt atacactctt ataggtctat ttctaaatgc gtattcaatg agttttatta
     3241 aagatgcaat gaattaccaa aaatctgtta tggtaatgtc aaatgaagtc gatttaatag
     3301 cagaagatat aatgaagagt ttagttagag gtgtaacttt tttagatgca gaaggtgcat
     3361 acactcatca aaaaaagaag attatatata caatagtttc atcaaatgag ataccaaaga
     3421 taaaagatat agctttgaag tatgataaaa aggcctttat atctgtaaat gatgttacag
     3481 aagtaaaagg aagagggttt aaagcaaaag atttatagaa aattttacaa gctgggagtt
     3541 tataatgtaa atttgtatat tatatctctc agcttattta aatttagtat acgaaaataa
     3601 aggttttatt aatgatttgt gtatcacttt aaaagatatc attttaaaat taaaattgaa
     3661 aaaattactt aatatgtaga aaacatgacc actattagga tataataaat ttaatagatt
     3721 tagtaaaata tctaggaata acgttggagg aatggcatta tgaatttatc taaagaatat
     3781 gttaatttgt atgataatta tttgaaatat ataaatgaag taaacaaaga gattagaagt
     3841 ataaagaaca tgaagaaacg tgttttaaaa aggggcgatt ttgctccgga aaatgaagta
     3901 ttagaattgg aaggaaaaac aatacgcgat atagatgatg tcgatacaaa aaaaccaaaa
     3961 aataaaatct ataaattttc aaatggggat gtatatgtag gaaaattctt agagggaaag
     4021 atggagggga aaggctcata tacttttttt gtagatgaag gaacagttat ggagtacata
     4081 ggagaattta aagaagataa aaaaaatgga aaaggaaact ttatattttc aaatggaaat
     4141 gaatatatag gttattttga ggaagatatg atgagcggta ttggaaatat gttatatagt
     4201 agtaaagatg aatatattgg aacttggaaa aacggaaaaa aagatggaaa aggtatttat
     4261 aagtggagtg atggatgtat atatgcaggt gaatttaaag gtggaaagat ggaagggtac
     4321 ggtatttgct ataatagtaa aggggaagta ctttatgaag gagaatggaa gaataatttg
     4381 attcatggta agggaactta tatatgggaa aaaggtaaaa agtatatagg tgaatttatg
     4441 catggtaaaa aacatgggca aggaactttt tatctaaata atgaattggt ttatgaaggg
     4501 acttggaaat ttgataagcc atctatattt gatagaacat tagatgaaat attttccttg
     4561 agactgtagc tttatctaat tagtagataa agcttttttc taatacttac tgtaaatagg
     4621 tatgaatgat attgattagt atacactatc aatattaatt taataaatgc aatattaaaa
     4681 aattttgatt gattttaaga aggagagata tattgaagag attaattgct ttagtgttag
     4741 tagtacttat tgttttattt tatagtcctg aattattaaa agaaggatat gatttaaaag
     4801 aaaagttttt tcaaagtgaa atatggaatg aatttttaga caaaataaat ataaatgata
     4861 attctaagaa agataaaaaa gaaaaaagtc aaaatttaca atcaaataat actgaagggg
     4921 agaacttagg agagtcagat ataaagaatt ttgaaaaaat aagcttagga gataatttaa
     4981 gctatgtttt atcaagtatt ggtaaacctg gtagaattga tattagtgaa tatggttttg
     5041 attggtatgt atataatcaa tatggaaaag aatttgcaat ggttggactt gaaaatgatg
     5101 aagtagtagc actttatagt aattctatta attcatgtga aaatcaagat ataaaactaa
     5161 atcaagatag acaaacagtg agaactaaga ttacaccatt aaaatataag agaaaaggaa
     5221 ataccagata tataataaac tcagaaaatc aatatgatat aataagtaag gaaggtaagt
     5281 atattacaat tttctacgat attcatgaag aaaatagagt ttgttcatac ttaataattg
     5341 ataaaagcac tgaagatgag tttaaaaatt tatatccaga tgatagcgaa gaacttaaaa
     5401 aatgttttga acttgaagtt atagaccttg taaatagtgt tagaaatcaa agagggttaa
     5461 actcattgag atacagtgaa caggcaactt tgagttcaag aaaacatagt gaagatatga
     5521 gagataataa tttttttgac catgtaaata agaagaatga aactccattt gacagaatga
     5581 aaagagaagg tatagtatat acttcagctg gagaaaacat agcagcagga cagattaatg
     5641 caatatatgc gcatgaagct tggatgaact ctgaaggaca tagaaaaaat attttgggga
     5701 actataataa tattggagtt ggagcaatat ttggaggaag ctataaaaca tattacactc
     5761 aaaattttta taaataacta aaatatttga aaggaagtta aaaaatgaca tcatggaaaa
     5821 aaaagacagt atataagtgc ttaatagcag tagtcttgtt ttgtggaata gtgttaatat
     5881 ctaattttag taaagtttct gctttgatga tggatactaa tggaaatgtt ttgattaaac
     5941 atggttcaag ggaaaaaaag ctaatagcaa ttacctttga tgatggtcct catcctaaag
     6001 aaactagtca agttttagat gtattaaaga aatacaatgt aaaagctact ttttttatag
     6061 ctggaaaaca tgcaaaatgg tataaagaac cattagtaag agcaagtaaa gaaggtcacg
     6121 agataggaaa tcatactttc aatcatcctg atataagtaa tttaagttct agtcagatag
     6181 aagaggagat tgttaagtgt gaagacattt tgaaagaagt aactggaaaa aaaccgacat
     6241 tatttagacc accatttgga agttatagag aaaaagaatt aatagaaatt gcgaagaagc
     6301 atgactataa agttgtttta tggacaggtg tagatgtgaa agattggaaa aatccaggag
     6361 ctaatagcat tgcagataaa ataattaata aagtacaaaa tggagatata atacttttgc
     6421 atgattatgc aactaatgat acagttgaag ctttagatat gtttattcca aaaatgattg
     6481 aaaaaggttt taaatttgta acagtttctg agttgataaa ataattttta ggggggagat
     6541 ttacagtatg attcttatga tagacaatta tgattctttt gtatacaacc tagtccaata
     6601 tatagaagaa ttaggggaaa cagttgtagt aaaaagaaat aatgaaatca aaataagtga
     6661 cattgaagaa ctaaatccag aggtaattgt gttatctcca ggaccgtgtt ctccaaaaga
     6721 agctggtatt tgcatagata ttgttgagca ttttaagggg aaaaaaccta tactaggaat
     6781 ttgtttaggt caccaaacta ttggacatgt ttttggagga gatattataa aagcacaaca
     6841 gcctgtgcat ggaaaagtat atagtatcaa ccatactaat aaaggagttt ttaggggact
     6901 taaaaatcct ttaaatgtca ctaggtatca ttcactaata gttgactcca atacagttcc
     6961 aaaagaatta gaaattacag caattactga taaaggggaa ataatgggga ttaggcataa
     7021 gaaatattta atagaaggag tacagtttca tccagaagct attttgtcag agtatggtca
     7081 tgaaatgctt aaaaatttta taacagaagc tagagaaagg gtacatatgt aatatgataa
     7141 gagagataaa tactaaatta aattcttttg agatatttac tatttttaga aatgaacatg
     7201 acagttttat attagacagt gctatggata aagaaaaatt gggaagatat tcttttataa
     7261 gtagccaacc atttaaggta ttaaagtata aagatactga tgaaaatcct ttagaagttt
     7321 taaaagaaga actacataag tatagggttg tgaatgatac taatcttcca tttgtaggag
     7381 gtgctgtggg atatttatct tatgatttag gtaattacat agagaattta cctagaactg
     7441 ctgtagatga tattgagatg cctgatatgt attttggatt ttataatcat gtaatagtta
     7501 tagaccatct tgtgcaaaaa acttatatag ctactccaaa catagatatc gaattagaag
     7561 aaaagataat agatgatata gaacaaagaa tattaaaaga agagaaaaaa ggtatagata
     7621 gcatatgtta tgaagaaaaa gaagtaacac ctataagact taaatctaat tttacaaagg
     7681 aagaatttaa aaatgcagtt caaagtgtta gagagtacat aagacaagga gatatatatc
     7741 aagctaattt aacacaaagg ttcagtggag aaacagaact tacaagcttt gaattgtata
     7801 gggacttaag aagatttagc ccagcaccat ttggggcatt cttaaacttt gaagatgcac
     7861 atatattatc aaattctcca gaaagattta taagatgtgt taataagaga atagaaacta
     7921 gaccaataaa aggtactcgt ccaagaggga aagataaaga agaagattta agacttcaac
     7981 aagaacttag aaatagtgaa aaagatagag ctgaactact tatgatagta gatttggaaa
     8041 gaaatgatat tggcagaata tctaaaactg gaagtgttaa agttccagag ctatttgtaa
     8101 ttgaaccata tgcaaatgta aatcacttag tttccacagt tgtaggtgag ttaaaagatg
     8161 ataaagatgc tactgatgta ataaaagcaa cttttccagg gggatctata acaggagcac
     8221 ctaagattag agcaatggaa attatagacg aattagagcc aacgcaaaga aatgtatata
     8281 ctggttcaat aggttatata ggttttaatg gtgatatgga ttttaatata gcaattagaa
     8341 caataatcaa aaatgacaaa aaagtctact ttcaagttgg aggaggaatg acttgggatt
     8401 ctgacccaga tgaagaatat caagaaacac ttgataaagc taaatccata atgaaggctt
     8461 tgaggggata ttatgaagaa taatgtaggt tttgatagta gtttaagtaa atttggaatt
     8521 ggtttgtttg aaactattag agttaaaaat ggaatagcag tagatttaga tatccatata
     8581 gaaagaatgc taagttcaat aaattgtttg gatttagata taaactataa aaaagatttc
     8641 ttgataaatg aaattgttac atatataaaa aaagaaaatg tcattaataa agcacttaga
     8701 attactgttt ttgatgaagg ttataatata tctattaggg atatttcata taatcaagag
     8761 acgtatgaga aggggtttaa attagctata tctccaatag ttagggggaa tagtttaatt
     8821 catagacata agactactaa ttattttgaa aacatatata ccaaaaatat cgcaaataag
     8881 actggttata atgatggtat atttttaaat tcagaagatg taatattaga atgttctatg
     8941 agcaatattt tttttataaa aggagaaaga gtgtacactc ctagtgatag attaccgata
     9001 ttaaatggaa taataaaaaa aaggattata gagatttgtg acgaattaca tatagagttg
     9061 atagaaaatg aaattaatat atcagaaatt agtagttttg attttgtttt tgtcacaaat
     9121 agtcttatgg gcgctataaa ggttacagaa attgacaaaa taaaatttaa taaagaaaat
     9181 gttgtttttg ataaaatagt gaagttatta gaataaatta aaatattaac aatttgttgg
     9241 agacaaaaga tgataggttg tatttatgaa gtatacaatg aagagataac taaaataata
     9301 gaaaaccaaa acactttggc tgtatattta gtgggttcta gtaaagacgt agattttact
     9361 ctttatgatg tagtgataaa cgatattgat gtgttcgttt ttgtaaaaga aggggaaaaa
     9421 caagagagaa tactgtttaa aaaaaaggga atagaatttg atgtaaatta cttttcaaaa
     9481 gatggtgtaa aaaaattatt atcaaatagg gaatatttct ttgtgaaaga aatgaaagat
     9541 gctaaagtac tgtttgatag ggagaatata tcacatatta taaaagatat aagtagagat
     9601 atttacttag aaggacctag taaaatgtca ttagaggaaa agtcttttat aaagcaagat
     9661 attggagcaa aaatatcgaa tttaaaaaat aaagaaaaat ttgatgtttt tgaatatcat
     9721 tttttgacga atttatattt aaaagatata ataattgggt actttaatat aaatgataaa
     9781 tgggtaccaa aagacaaaaa attgctaaaa gttttaaaaa aagaaaataa tgagcttttt
     9841 gaactagtgt caaaagtaag tgaaaattat gactatcaaa gacttttaga tgtttataat
     9901 tatattttta aagaaattga gacaaaagag gttataaaac taatcttcta gtcacaaatc
     9961 aatgggggga gtttaaatga ataaagttga taaagaaaag atacaacatg cagtaagaga
    10021 aatactagag gcaattgggg aagacccaga tagagaaggt ctaatcgaaa ctccaaatag
    10081 agttgctagg atgtatgaag agatattctc tggtcttagt gaagaaccaa gagaccattt
    10141 aaaagttctt tttgcagatg aaaaacatga agagttagta ttggtaaaag atattccatt
    10201 ttattcatgt tgtgagcatc atctagttcc tttctttgga aaagctcata ttgcttattt
    10261 accaaaagga ggaagactta cagggctttc taaacttgct agagtaatag atacattggc
    10321 aaaaagacca caattacaag aaagaatcac taaaaatgct gcagatataa taatggaaga
    10381 attacaacct tatggtgtct tagtagtagt agaagctgaa catatgtgca tgacaatgag
    10441 aggcgtaaaa aaaccaggtt caaaaactgt tacatcagca gtgagaggaa tatttgaaaa
    10501 ggacattgcg tctagagccg aggctatgag tttgattact atgaagtagc aatttattat
    10561 agatataatc atttattgag ataaaattta atttaaggga gtgtcattaa ttgtgtttga
    10621 ttatggtaaa agaacttata taatgggaat attaaatgta actccagata gtttttctga
    10681 tggaggagat tttaataact tggacatagc aatacaacat gcaaaagata tggttgacca
    10741 aggtgcagat ataatagacc taggaggaga gtctacacgc ccaggtcata gctatgtaga
    10801 ctcagacgaa gagctaagaa gagtaatccc tgtaataaaa aaacttaaac aggaattgga
    10861 tataccaata tcaatagaca cttataaggc agatgttgca gaagaagcat taaaattagg
    10921 tgtcactatg gtaaatgatg tttggggtct tagaaaagat aaaaatatgg cttctgtaat
    10981 aggaaaatat gatgcagaag tgtgtataat gcataatcaa gatgggacaa actatgacaa
    11041 agatataatg gagtcaataa aagacttttt taaggtaagt atagaaatgg caatgagttg
    11101 tggtgtaaaa aaagaaaaaa tagtacttga cccaggtgta ggatttggca aggattttga
    11161 acaaaatatt gaagtattga gaagattaaa tgaattaaaa gatttagggt atcctatttt
    11221 gcttgggaca tccagaaaat cagtaatagg aaaagtactt ccagttgagc ctaaaaaaag
    11281 attagaaggt acaatagcta ctactgttct tggtataaga gatggcgtag atatagttag
    11341 agtacatgat gtttatgaaa atttaatggc agctagaatg actgatgcaa tatatagaaa
    11401 gtaggtataa tataatggat aaaatattac ttagtaatct aggattctat ggatatcatg
    11461 gtgttcttaa agaagaaaat tttctaggtc agaaattctt tgtcgacatg gaactttata
    11521 ttgattcaag agaagcaggt cttagtgacg atataaataa atctgtaagc tatgccgagg
    11581 tttacaatgt tgttaaagat attactgaaa ataaacaatt taatcttttg gaggctttag
    11641 cggaaaatat tgctgaagaa gtcttgaata aatttatact tataaatgga gttatggtca
    11701 gagttagaaa gcctgaggct cctgtaaatg gtatttatga ctattttggt gttgagataa
    11761 ggagaacaag agatgaataa ggcttatttg ggaataggta ctaacatggg tgatagattt
    11821 gataatttat caagagcatg tgaacttttg aaaaatagtg attctatata taaagtaaaa
    11881 gaatcaagct tatacgaaac aaaaccatgg ggatatacag aacaagcaga ttttctaaat
    11941 atgtgtgtag aaatagaaac agagtttgaa ccttatgagt tgttagaata ttgtcaagaa
    12001 atagagagag aactacacag agagagaata gttcattggg gaccaagaac tatagatgta
    12061 gacgtactat tttttaatga tgtagtttcc actgatgaaa gactaacaat acctcatcca
    12121 aggattcaag atagagcttt cgttttgata ccacttatgg atttaaatga aaaacttata
    12181 ataaatgaaa aaacaataaa agaacattta aacctcctat ctgctgaaga aagagaagaa
    12241 gtaaaggagc ttgtaggtta tgaaagaaaa cctatttaat aataattcaa agatagagat
    12301 aaatacaaat aacaaaaaga taactgtaaa aaaagataaa cttataatag ctggaccatg
    12361 cgcaatagag agttatgagc aacttttgga gactgctaaa tttgtaaaac gtcagggagc
    12421 aaatatttta agaggtggag catacaagcc aagaacatcg ccaaattcat ttcaaggatt
    12481 aaaaaaagaa ggtcttgaaa tattaaaagc agttaaagac gaagttggta tggctgttat
    12541 tacagaactt atggatgtaa gagatatgga cgaactgtat agtataagtg atataattca
    12601 aattgggtca agaaatatgc aaaactttac tcttttaagt gaagtgggaa aacaaaataa
    12661 acctgttatg ttaaaaagag gaatagcatc cacaattact gaatggatag gtgcttctga
    12721 atatatagca atagaaggaa atagtaatat tataatgtgt gagagaggta taagaacgta
    12781 taatgattat actagaaata cattagattt agctgcagta cccattattc aaaaggaaac
    12841 aggtcttcct gttgtggtag acccaagcca tgccactgga gttagatatt tggtaaaacc
    12901 tatgtcactg gcatcttttg catgtggagc agatggaata atggtagaag tacatcctga
    12961 cccagaaaat gctttgtcag atggtgctca atctctttgc tttaatgaat ttgaagattt
    13021 aatgaaatca attaataatt attaaaagag gcaagagcct cttttttatt cgtaatcgtt
    13081 gctaaggtaa atttataagt tataatttga tagttagatt atttaatata aataaagatt
    13141 taatattgat agaattaata taaaaaataa aaattttttg attaataaga aggatatcaa
    13201 taaaaaaagt cgaattgtgt atattgagtg attgacaaat ttagaatata gtgtagaata
    13261 gaaatgttaa cacaaaaaat tacaccagaa ggtgataata tgaatgatat aaaagacatt
    13321 attgaagaaa taaaagctag atgcgatata gcaagtataa tatctgaata tatgaatata
    13381 aaacaatctg gagctaatta caaaggatta tgtccatttc atggcgaaaa gacaccttct
    13441 ttttatataa atacgtcaaa acagatttat aagtgttttg gttgtggtga aggtggagac
    13501 gtaataaatt ttgtcatgaa aatggaaaat ttggatttta tggatgctgt aaaaatttta
    13561 gcaaataaat gcggtataga aattaacact aacatgaatg aagaaactag gattaaaata
    13621 gaaaaatcta agaaatttca agatattcat acggaagcag ctagatttta tctttcaaac
    13681 ttattgggaa gcaaaaatct tgggtatgaa tatttaagaa taagaggact agatgataaa
    13741 attattaaga aatttggttt aggattttct ttggattcat ggaattcact tatgaatact
    13801 ctaatcagta aaggatataa aaaacaagac ttattggagt gcggtctaat tgcaaaaaat
    13861 agagatggaa ctaattgcta tgataaattt agaaatagag ttatgtttcc tatattcgat
    13921 tatagaggaa atataatagg atttggggga agagtattag acgattcttt gccaaaatac
    13981 ttaaattctc cagacacatt aatttttaat aaaaaacaga acttatacgg attaaatttt
    14041 gctagaaaaa atttagaaag taaaactata gtcttagttg aaggatatat ggatttaatt
    14101 tcactttatc agtatggtat aaaaaatgta gtagcaaccc ttggaacagc cttgactgaa
    14161 caacaaggca ttttaataaa gagatatgcg gacactgcta ttatatctta tgattctgat
    14221 gaagcaggaa taaaagcgac tttaagagct atagatatac ttactaaatt aggtatcaat
    14281 gtaaaagtgt tagacttaaa agatgctaaa gacccagatg aatttgtaag aaaatacgga
    14341 cttagtgatt ataaaaaagc tatggatgtt tctactcact acataaaata taaaatagat
    14401 catcttaaaa aggaatttaa cattcaaaaa gatgaagaac gagtgaagtt cgcaaaagaa
    14461 gcttctaaaa ttataaaaca attgacaagt cctgttgaaa ttgattttta tacaaagtat
    14521 ttaagcaatc aaatagatat taatgtcgag tctataaaga gagaagttta tggtaaaaat
    14581 tacaataagc catataataa caaaaatcaa aagaaaatag aagaaaaagt aattgaaaaa
    14641 gtcgaagtca gacaagacgg aaaacaatta gttgaagaaa ctctaataaa aataatgtta
    14701 gaagacaaaa aaattagaga aatagcgtta cttaaagtag aagaaagtga ttttttattg
    14761 aaagaaagta aagaaatttt aaattacatg attaaaaatc aagaattgga caaaataact
    14821 attgacaaat tgaaaagttt aaacatatcc gaagaatact taaaagaatt aaattcaatt
    14881 tctttgaata gtataaactt agagaacaca aaagaaatag aaggaataat aacaaatatc
    14941 agaaaaaact cattagaaga gcaaattaat agcttgttga gagaacaaca agaattagaa
    15001 aataataatg atatgaaaga ggtagatggt agagttatgg aaattgcttt aaaaatagtt
    15061 gagataaata aaattttaaa gagcttgtag agaaaggagg tttgagtgtg gaaaataaat
    15121 cgaataaaaa agagttaaaa aaggttactg ctaagacatt aatagaaaaa ggaaaaaagc
    15181 aaggttcttt gacacttgca gagataatgg aagctttttc ggagactgaa cttgataagg
    15241 atcaagtaga aaatctttat gagactttag gaaatttggg aatagaaata acagaaacaa
    15301 aaaactataa agctgatata gatttttcgg ttgctgatga cgatttaagt ataggccact
    15361 tagatgaaga tgcagaggca atttcacatg atgactcttc tgcaatagaa atagaaactg
    15421 tggatttatc tttaccaaaa gggataagta tagatgaccc tgtaagaatg tacttaaaag
    15481 aaataggaaa aattcctcta cttaaaccac atgaagaagt agaatttgct agaaggatgc
    15541 acgaaggtga tgagatagca aaacaaaggt tagttgaagc aaacttaaga ctagttgtaa
    15601 gtatagcaaa aagatatgta ggaagaggta tgcttttctt ggatttaata caagagggaa
    15661 atttaggtct tataaaagca gtcgaaaaat ttgactatac aaaaggatat aagtttagta
    15721 cttatgcaac atggtggata agacaagcta taacacgtgc tattgcagac caagctagaa
    15781 ctataagaat accagttcat atggtagaga ctataaataa gctaataaga gtatcaagac
    15841 aactacttca agaacttgga agagatccaa aaccagaaga aattgcaaaa gaaatggaaa
    15901 tgacagaaga taaagtaaga gaaataatga aaatagctca agaccctgtg tctttagaaa
    15961 caccaatagg agaagaagaa gatagccatt taggtgactt tattccagat gatgatgctc
    16021 cagccccagc agaggcagca gcatattctt tattaaaaga acaaatagaa gatgtacttg
    16081 gttcattaaa tgatagagaa caaaaagtat taaagcttag atttggtctt gaggatggta
    16141 gagccagaac tcttgaggaa gttggaaaag agtttgatgt aactagagag agaataagac
    16201 agatagaagc aaaagcacta agaaaactaa gacatccaag tagatcaaaa aaacttagag
    16261 attatttaga ctaaaaagat acatcattga tgtttttgtg ataaatgcca tgagataagt
    16321 gatatctcat ggctattttg tagtaaagat ttaattttat ttttgtagtt atttcagtct
    16381 aatatctata acttggatta aatcctataa ataaagggaa ttgaaaggag aattatattt
    16441 gaaattaaca gatagattat tgaaaatagc atcattagtg tcagatggta aaaaaatagc
    16501 tgatatagga acggaccacg gatacatacc agtatatttg ttaaaagagg gaaaagtacc
    16561 atttgcagtg ctagcggatg ttaataaagg acctcttgac aatgcacata aagaagtcat
    16621 acagaacaat cttttagata aagttgattt acgattaggt tctggtatag agatacttga
    16681 aataggggaa gtagaagaag tgataattgc tggtatgggt ggaatcttga taagtgagct
    16741 tctcgaggct aaaaaagaag tagcacataa tgtagaaaaa ttaatattac agccaatgca
    16801 agcacaagaa gaacttagat attacctttt aaacaacgga tatgaaattt tagaagaagt
    16861 tttagtgaga gaagatttta gaatatatga gataattgtt gccaaatata caggtaaaaa
    16921 tactataata gaagatgaga tatattatga agttggaata aagcttttag aaaacaaaga
    16981 ttctcttttt aatgatttta tagaaaaaaa gataaagaca tatagttcta tagttaataa
    17041 attagaaggg aaaaatgggg aagctataga taagaagaga aaggaaagcg aagttgcaat
    17101 aaaaaaactt gaaaatttaa taaaataaat aggaggtata tatgctatta aagagtctaa
    17161 ctagaaaaat tgaaaaaaag tatcctctaa acttagcaga ggattgggat aatgtaggtc
    17221 ttatagttgg agattttgat atggatgtaa aaaaggtatt ggtatcttta gaagctaatg
    17281 aagatgttat tgatgaagct atatccaaaa atatagattt aattgtgaca catcatcctt
    17341 ttatatttgg gaaaattaat aaaattaata gtggagattt aaaaggcaga cttatccaaa
    17401 aacttattaa aaatgatata tcactttatt ctatgcatac gaattttgat atagcatttg
    17461 atggtctaaa tgactatttt atggaaatta tggaatttgg taattcaaag gttttagata
    17521 taactaaaag cgaaaatttg tataaattag cagtatacgt tccacataac tattcagatg
    17581 aacttagaaa ggttttaagt aattcaggag caggtcatat agggaattat agtgattgta
    17641 cgttttcaac agaaggtgaa ggacaattta agccattaga aggctctaat ccgtttcttg
    17701 gaagtgtaaa tgatatagaa actgtaaatg aagttaaaat tgaaactgta gtaccacaga
    17761 agcttttagg tggggtaata agttctatgt tagatgcaca tccttatgaa gaagttgcat
    17821 atgatttgta caagttagaa aataaaggtg agatatttgg actaggtaga ataagtaaat
    17881 ttgataaaag tatgacatta gaaagtcttt caaggaaaat aaaagagaaa ttaaatatga
    17941 aacacataag agtagttgga aaccttagta cagacataac gaaagtagcc gttgtaacag
    18001 gagctggttc agaatttgta aaaaaagcta agagacaagg tgcagaagta ctaattacgg
    18061 gtgatgtaaa gtatcatgag gcacaagatg cacttgatat tggaatgtgt atagtggatt
    18121 gtggtcattt tgatacagaa gatatattca aaaatgttat gaagagattt ttagatgaat
    18181 ttagtgaaat tgaagtaata aagagtaatg tatatttgaa tccatttagt actatttagc
    18241 agtgtttgat aataaaaata ctatataaaa aataaatttg agtttaatta gggggataat
    18301 gatgaaagta gcagtaattt ttcatagtgt ttgtgggagt acatatttat tagctagaga
    18361 atataaagaa gcgcttgaag aaatgaatat cgaggtagat atatttagag taagtgacga
    18421 agttgcaaaa acacttcctc aatattattt gataaattcc aaagaataca aagacgaatt
    18481 tgaaagtata aatgtaatta aatctggaaa agaaatatta gattatgatg ctatatttat
    18541 gggttcacca acatattatg gaaatgtatc tggaccaatg aagatgttta tggatagttt
    18601 ttctgatatt tgggtaggtg ctcctttgag tggtaaaata tttggttgtt ttgctacagc
    18661 aggttcacaa catggaggag gagaattagc tttgcaagct atgaatatat ttgcccaaca
    18721 tatgggaatg actcttttgt cagttccatg ttcagtaaga ggaggttatc cagcgtatgg
    18781 aatacttcat atagcaggag ataattctga tataagacca aatgatgatg ccaagatagg
    18841 tataagagat tacctaaaaa gacttaacat ataagaatat catataaaaa tacctaaaag
    18901 cgactgtata tacttttagg tatttttact taattataaa cgtgaagctt taccagaaat
    18961 atggtcaatt tctattttaa caacagttgt taagttcata tccttctcta tgtacttcat
    19021 gccttcttca aagaatcctt tagaatattt ttttattatt tctacaagag catttttctt
    19081 ttcttcattt tctacagtgc aagcttttcc aaaaactata gcactagaat aagtagtact
    19141 aaatttatct ggaataacac tttcattagc aactactaaa aaagaaactt tgttgttttt
    19201 cgatatatta tctaacttat gcccatttct tgcacaatga aagtaaatag aatcattaaa
    19261 gtatacataa tttacagcaa ctccataagg ataaccattt tcagatatag tggaaaaagt
    19321 accaaattca ccatttttta atacttcaac agtatcttct tttgtcattt ctcttttttt
    19381 caatctcatt tctttaaaca taatatcatc ctttctaatt ttgatgctta aattaaattt
    19441 tagaacattt ataaagatta tgctatgttt tttacaaatt ttaagaaaaa aatatagttt
    19501 aagataaaag gtagtgaaag agaagctatt ttgaggttta tttatgatat tattgttaga
    19561 gtagtacaaa caatactaat aaatttaata gctggtttct tgttatttaa ggtagtaagc
    19621 aggtctataa gaaggggaag ttatcgaggg tagaagttgt tgtttacaac gatattataa
    19681 agaaaatatt attatataaa ataataaata gtaaagaggt atcaagttga atataatact
    19741 taatacctct tcaaaattaa gttataaaag tgttgtggga attgtcctat acatggtaag
    19801 gagataataa agaatgtatc tttaaagtct caactaaaca gcaaaattta tccaacaaac
    19861 atacaataaa tgcttctttg tatctaggtg gcttaacagt aagtggccac atgtgtttta
    19921 aaattatgtc cgtttctttt tcatttattt gaaaaaataa agttgcattt tttaatgctt
    19981 ctctagggtg agtaaatcca tgtaatcctt ttctatcacc tttgtaatgc caatcataaa
    20041 gaaaaaagtc gtgtagtaaa gctcctctaa taacactttg aatatcaaca cttaagtgca
    20101 attttttaca taatagataa ctataatatg ctactgacaa actatgttca agacatgaaa
    20161 cattgccatg atgaggaaat gttctcataa gttgaacttt ttcagaagaa agtatctcag
    20221 aagcacattc attaaaatat ttgatttcag cttttgacaa cattatgaaa atctccattc
    20281 ttagtaatat ataaatacta tacttcaacg ataatatgaa atatttctta tcctacaaaa
    20341 tataataccc aataatacaa gtaagatacc ttttatggta ttttaaagta agagagattt
    20401 tattaggcta aacaatgaaa aaattaaaag taaagctata gcaatatata gtatttttcc
    20461 aagaggattg ccaaaattta tagtccatcc aataccaaac cttttattaa ccattaatga
    20521 tgggtcattt ggattattat acatgatgcc tgctatccaa tacttctcat cttcttctgg
    20581 agaataagat gagttgaatt ttaaatttgg ggaattaata tatgttgcta ttaaataaat
    20641 tataactaaa agcgttgtaa tattcatagc agttgatagt tttgttttta ttgataaaag
    20701 agtagtaaaa aactgtgttg tcattataat catcaatatt aaaaacaaat aaccaatttt
    20761 atttaaatat ttaatgtttt caactctact ttcttcaatt ctatttgtat ctatcttaat
    20821 ccttgacttt atggatccaa tggaagttat atataataaa accattaaac aaaagtcagt
    20881 accgattaat ttaaatacat taagaaatga tttatctata aaaacatcag gagacccatt
    20941 tatgttccaa tgagtggcaa ttgagtctgg caattgatta tagttaaatg ctataaaaat
    21001 actcattccc aaagtaaata gtatcggtat taaatagatt attttaaatt tttttattat
    21061 tttatttttc ttatttataa aatccatatc tactattaat tttgaatcta tatctatatg
    21121 acctaattta gcataaattt ctgatttaag cttcttagct tttttatgtg tatgaatata
    21181 tagtatactt tcatataata aaaaacctat aataccaata aaataggaaa gttccaattt
    21241 attaaatgta aaaaccagaa taaaaactaa tataaagtct ataataaaac ctaaagataa
    21301 tagtttttta aattttttat ctaactttat aaagtaatta taatttttat gctcttggtc
    21361 tatagaaact ccatagatat gttttttacc tacaaaatag ggcataaaat aaaatgtagt
    21421 atacatcaaa attagaactg gaaatattgt aaatagacat tcaatcttac tcataatatt
    21481 tcacctcata cttgtcaaat aaacttgaac ttagtgacaa aaattcttct ttactaatac
    21541 ctgataaata agattttgcc accaataatt ccaagtcaag tttatttttt tgacgagttg
    21601 tattatctat tttcattggt agttgagcta caatagcacc ttttcttcta tcaatattta
    21661 agaatccctc ttcttttaat tcattgtagg atttgttcac tgtatgtaaa tttacaccaa
    21721 tattttcagc catacttctt acagatggaa gtgattcatt tattttcaat tctcctgaag
    21781 ctatagcctt agttatttct tctttaattt gtacataaat agatttatca ctattaaaat
    21841 ctaaatttaa aaccataaaa aacctccatt atgctttata aatgaaaacg attgttatat
    21901 acatagtata acataaaaaa taataacaaa cacaaaatat aagtttatat tttgtaaaaa
    21961 ttgtaattta aaaaataaca taatttttat taaaataata cagatactat ttatagatag
    22021 aaataaaaat tagaaaggag aaaaaatatg ttaacatatt taatgaaaag acaaatgcat
    22081 aaaaaagatg atggaatgag taaaccaatg atgattacag gagctgtaat tacaggtgta
    22141 ggagcatatg ctgtatataa aatggtaaaa agccataaat ctaattctca aatggtcaat
    22201 acaaattatt atggaaatag cgattatgat gattataaat atgacgaatt tgattatgac
    22261 tgtgattgta gagacacaaa acaagattat gaatatgaag aattaaaaaa aaaagtaaaa
    22321 gaatttaatt caagaagaat aaattgtgaa aattgtccac atgtttctca agaagaaatg
    22381 atgcaatatg taaatagtgt taaagatgta aaggaagaga ttgatttaca tgaagatgct
    22441 aaaaaagata atatttacaa agaagatgaa tacaaaaaat atgaagaaga ataagtaata
    22501 ttttgtataa ctgaacaaat agtaatgtca aattatttaa tatctagttt taatatgtaa
    22561 gttgtaactt aacttgaaag aatcagtatc attctttaga tagttaagta acaacttaca
    22621 ttttattttt ataaatttta atagttcttt gtgactattt tgtaatttta gaaatatttt
    22681 agtatccagg gtaattgtgt ataataaaac ttgtatagat agatagtatg aaattaagca
    22741 gatagtttaa aattaaaatg aaaagaaagg aaatagttat gttaaagttg ttgatagttg
    22801 aagatgatag tactatatct tttggaataa agtatgcttt agaacaagaa ggatttagta
    22861 tagatatatc aaaggattta tctagtggaa aagaaatgat ttcttctaat gaatatagca
    22921 tgatactgtt ggatgttaca ttgccagatg gaactggtta tgagctttgt caatatataa
    22981 gaggattctc tcaggttcct ataatattct tgactgcttg tgatgaagaa gtaaatattg
    23041 taatgggtct cgatataggt ggagatgatt atataacaaa accttttaga attagagaat
    23101 taatatccag aataaaagct gttttaagaa gaaaaggtaa tacttcagaa gaaaataaga
    23161 aaatacttaa atttggagat ttaagtatat atacattaga agctagagta tataaaaatg
    23221 ataaagaaat atttctgaca tcagttgaat ataaacttct tttaattctt atacagaata
    23281 aaaatactgt tttaagtaga actcaaatat tggaaaaact ttgggatgta acttatgatt
    23341 ttgtaaatga caatactctc actgtttata taaaaaggct tagagaaaaa ataggtgatg
    23401 atttatgtga accaatctac atagaaactg taaggggaat tggttataaa tggataggaa
    23461 gtgaaaatag tgtttctata taatccagaa gtgaagaagt ttttaagtaa gtatgttact
    23521 ttgatgttta ttgttatcat aatatctatt ggatttagca taatcaacgt gtccttaact
    23581 aaagagatgg ttgtaagaaa taatcaggct ataataggaa ctttatcaag caagtatcca
    23641 aacttagaaa gtcagatagt agacataata actcaaggga agtctatgga gaacatagat
    23701 tatggtcaga aaatattaat taagtataat tatgataaat ctattaggat aaactctgag
    23761 cctattattt ctaaattagt tttagagact atcaaaataa atataatatt agtatgtata
    23821 atatttattt tgatatttgt gttggttgtt agatatttta agtcaattta caatgattta
    23881 tcagatatga ctaagtatgt ttactatagt tcagagggta agccatttga tatgaaaaac
    23941 aagaatcaag aagggcagat aggtcttcta aaaactgaac tcttaaaaat gacgacaatt
    24001 cttaatgaaa aggttgaact tttaaaaaga gaaaagatat ttttaaacaa tacaatttcc
    24061 gatatatcac atcaattaaa aactcctatg acttctttaa taatgttgaa tgatttatta
    24121 tataatgatg taccatacga agtaaaaatc gattttttaa ataagataaa aaatcaatta
    24181 aatagaatgg attggttgat taaaagcatg cttaaattat caaaggtaga agcaaaagtg
    24241 ataaatttta aaaaagataa ggtaaaattt agtgaactca tacatagagc aatgctatct
    24301 atgaaaatac ctatggagat taaaaatcaa aaactgatta tagagggaaa tgataatgta
    24361 tcttatattg gtgatattga ttggtccgtg gaagcattag tgaatataat caaaaattgt
    24421 gtagaacata ctcctgaatt tggaaatata attataacct ataaagaaaa tccattattt
    24481 tctgaactaa taataaaaga tgagggtgaa ggtatagata aaaaagatat accacatgta
    24541 tttaaacgat tctatagagg aagaagtagt tcaaaagaag atagtgtagg aataggtctt
    24601 gcaatgtcaa aatctataat agaaagccaa aatggcgata tatatgtaaa cagcgaaaaa
    24661 ggtaagggta cagaatttca tataatattt cataaaatgt atgatagtga ctaatctgta
    24721 atatttaatt cactttatag taatagccaa aacctaatat atgaagtata gaatataaat
    24781 attggaggta gttatggaaa ttttaagagt agaaaatata actaaaagtt atggtaaaaa
    24841 tgaaacaaaa gttgatgcta taaaaaatgt aagtttatca gttgaaaaag gaacgtttat
    24901 agcaataaca ggtccaagtg gaagtggtaa aagtacctta ttacatttat taggaggagt
    24961 tgacagacca actagtggta aggtttatat aaatgatgtt gatatctata atttaaaaac
    25021 taaggatttg gcgatattta gaagaagaaa tataggtctt atatatcaat tttacaactt
    25081 aattccagtc ttaaatgtga aggaaaatat attgcttcca gcagagcttg acaatagaga
    25141 tattgataga gaatattttg atgatttaat gaaaacatta ggtctaattg atagagaaaa
    25201 gcaccttcca aaccaattat caggaggtca acaacagagg acctctatag gacgtgcatt
    25261 aattaataga ccttctataa tattagcaga tgagccaaca ggtaatttag atagtaaaaa
    25321 ttcaaaggaa atattagaat tgttaaaatt gtcagtaaaa aaatacaacc agacactaat
    25381 aatgataact catgacaaaa atatggcact tcaagctgat agaattataa gcatagaaga
    25441 tggaatcata aaaagtgatg aggtgatgta gatgaattta tatacatcac taactattag
    25501 atacttaaaa caaaataaaa gaagaaccat tgtaaccata atcggtataa tactttcaac
    25561 agctttaatt tgtggtatag gaaatatatt tgaaagtttt atggattatc aaatgagaga
    25621 aactataaag aatgatggaa gtttccatgt tacattttat gatgtaaata gaagaaatgt
    25681 ggaatatgta acgaaatcag ctgaaataga aaaacatgca tttaccaaac aacttggata
    25741 ttctaaatta gaaaatagtg aaaatgctat attaagtata aagcaatatg ataaaaatgc
    25801 aataaacgga tataaagttt ctataaaaga aggtagattt ccagctaagg aaggagaaat
    25861 tgttttaagt gaaagtatta ttaatcttat agatgataag ttaaaaatag gagataagat
    25921 aactttaaaa gtaggggata tgtttgactc aagcaagaaa aagattgata gtatgacatt
    25981 tcatgaaggt gattatatag caaatgaaaa agatagaact tttaaagttg ttggtataat
    26041 tgaaaaacct gggtttgaac gttataatgg aattgcaaca gctatagcct actttaatcc
    26101 tatgaataat tacaatgata caattaatgt atcagtagca gtaagaaatc ctaaagatgt
    26161 gtataagata agtaataaaa taagtaaaaa tatatattca aataaagata aagaagcagt
    26221 ttctgatatg ataaaataca atgaacattt attgaggtta caaggtgcta gtaagtattc
    26281 aaatataaat agctctataa agtctataat tatagcagta actattttag taatcatatg
    26341 tacaattgct acagtgtaca attcctttag tatttctata aatgaacgaa aaaaacagtt
    26401 tgggatatta aattctatag gtgctacatc tagtcaaatt aaaagattag tttttataga
    26461 aggtattata attagtttaa ttggtatacc aataggacta atatctggaa cagtagctat
    26521 agacttattg tttaaaatta taaataagta ttttactgaa tcggttgtta ctaagatgag
    26581 tttgcagata gtgtataatc caataataat tattgttagt ataataatag ttttgtttac
    26641 aatctttata tcaattctac tacctgcaat atcggcttct aatatttctc cccttaatgt
    26701 gattaaaaat actggagagt ataaagtggg caaaatcaaa agctcaagac ttattaaaat
    26761 gatttttaaa actgaaggag tacttgcata taaaaatatt aggagaaata gaaaaaaatt
    26821 tataataact cttttttctt taatggtaag tataataata tttatatctt ttagtggctt
    26881 tacattactt ttactaagag gtgaagaaat tagaaattct caaagaaaat atgatttgta
    26941 tttaactgca aagaacacag ctaggtctgt agaggatact ataagtgaac tagaagatat
    27001 tgatgggata aaaaactttg aattggcaac aggacaatat gtgtctataa gagtaagtga
    27061 aaataaaata aataaatcca aggaagattt aatcaggaaa tattatcaaa aatataagat
    27121 tggagatagt tatgaatatg atttcagtaa taatgaatta atttttccag gagattttgc
    27181 agtaaaaaac aaaataaata acttagtaca gggctctttt aataaagaga gagcaattga
    27241 agaaaatggt gttatattag ttagaaaaag tgcatttgaa gaaaatggga agaaaggtgt
    27301 agtagaactt accaactata aagtaggaga tacagtaaat tgtgaatatc ttgatgaaaa
    27361 tgccttaagt aaaaaagtaa aggtcaagat tcttgctata actgatgaag aacgtctagg
    27421 tcttggttat caaaatatgg ggttacaatt tataacttat gatgaagttg ctaaaaattt
    27481 aaatttaaaa ttaagtaaaa gtcttatatt tattgattca ggaggaagca ttaagactaa
    27541 gaaggaaata gaagctttgg caaataaaaa taattttaac ttttatgatg aaagtgcaag
    27601 caatgaaagt gaaaaacaag atttaaaggt tattaaaata tttgtatatg gttttatagt
    27661 agtgatatct ttagtgagtg ttacaaatat tctaaataca gtaagtacaa gtataaatct
    27721 aagaaaaaga gaacttgcag taatacaatc tataggggtt actcctaaag gatttaggaa
    27781 aatgatatat ttagaaagtt ttatatatgg tatactgtca ttgttgtttg gaataccaat
    27841 tagtattggt ataattctaa ttatgaataa acttatttca ggtgtaattg aattttctcc
    27901 gattataccg tggacagcca tagttatctg tatagtaagt gtttttataa taacttttat
    27961 agcagggtat ataccaatga gtaaattaaa taaagaaaat ataatagata atataagaag
    28021 agaaagtata tagatataaa aataattctt aactatataa aatattatta aaatcattta
    28081 agtggtataa aatattatta tatcacttaa atgatttttt atttatataa gaaatctaat
    28141 aataaattac tagaggtatt taactattta gaaagaattg gtattatata aagtaagaga
    28201 atatataata agaaagaata tttgatttta ttggaaaaca aaagaaaaag aaggtgatta
    28261 ctatttctca tataactgcg aagaatatgt ataaaagttt agaggaaaga ataaacaagt
    28321 ttccacaagg tgctccacca tctgatacat tatacaaaat actaaatgta ctatatacag
    28381 aacaagaggc gaagttagtt gctcagcttc caataaagcc tttcagagta aaaacagcag
    28441 ctaaaatttg gagtgtaagt gaaagtgaag catatagagt attggataaa cttgctagta
    28501 aggctctgat actagatata gaagataata aagggaagaa atatataatg cctcctccaa
    28561 tggctggttt ttttgagttt gccatgatga gaacgagaca cgatatagac caaaaacttt
    28621 tggcagagct ttactatcaa tatatgaatg tagaagaaga ttttatcaaa gatttatttt
    28681 attcaacaga aacgaagctt ggaagagtat acgttcaaga ggaagtttta actaatgata
    28741 atgaggttag tattttagac tatgaaaggg ctactcatat aattgatgaa tcaactcata
    28801 taggtataag tatgtgttac tgtagacata gaatgcaaca tgttggaaaa gcttgtgatg
    28861 ctcccatgga tatatgtatg acgtttgaca atgtagcaaa ttctttaata aataataaat
    28921 ttgcaagacg tgtggataaa atagaatgta aagaactact tcatcaagca tatgaacata
    28981 acttagttca atgtggagaa aatgttagga aaggggttac gtttatatgt aattgctgtg
    29041 gatgttgctg tgaagcaatg gtagcagcta aaagatttgg aaatcttcat ccagtacaga
    29101 caacaagttt tataccaaat ataaaccatg aaaattgtgt aaagtgtggt aaatgtataa
    29161 ctgcatgtcc aatagatgcg ataagcaagg ttaaggaaga tggtaaagaa tatataaaaa
    29221 ttgatgagga tagatgttta ggttgtggag tttgtgttag aaattgccat aaaaatagca
    29281 ttatgttatt aaagagagat gaaaaaataa taactccagc aaattcagta catagagcag
    29341 ttcttatggc aattgaaaaa gggcagttac aaaatttaat ttttgataat aatgctctgg
    29401 ctagtcatag agctatggga gctatactgt cagctatatt aaagcttgaa ccagctaaaa
    29461 agattttagc cagtaaacaa ttaaagtcag tatatttaga taaattgtta agtatgaatg
    29521 ataaatagaa actaacttaa aaaaggcaaa gttttagact tatctatatc ttaataaact
    29581 tatcatttaa tatttaaatt taatttttac acatataaaa cttataactt aaataaaaaa
    29641 aggctatatt caattaatat tacttgaata cagccttttt tagttatgtt aagctatttc
    29701 ttttatttgc ttaaatgagt tttcatttcc atttccacca atttgtgtta cagatttaaa
    29761 tctcatagtt ttaaatagtt ctttttgatt ataatctagt gagtttccta ctagcattac
    29821 aggagattta gtttttccag ccaaaactcc aacagataat ccgtctatta aatcatcttg
    29881 tttattcatt ccattttttg caacatataa attgtctatc ttagaatcct tgtaaaattc
    29941 ttttattact tttgcattag tttcatttct agtgctacct gatattctct gtggattttg
    30001 aagtttactt tctatatttt tagaaactgt atattctcca ccgattacat aagatttatc
    30061 aatctttttg tttttaaata agttatttat ttcagttata ttactatttt cattagtaag
    30121 tattataggc atatcatttt gagctgatac agctccaatg cttactgcat cggctaatcc
    30181 tttttcgcca ttaactactg aaatctttga tatattgcta atattgttca attctttagc
    30241 aatgttcata gaagtttgat atctatcact tcctgaaatt ctagatacag agatattgtt
    30301 tgattttata gtatctaaag atttttcatt tatagaagct tctccaccaa ctacatatac
    30361 atttttaact tttaatctct ttaattcagc caaagttttt tcatttattt gagagcttcc
    30421 agtcaataat ataggagcgt ttttcttgta tgcaaatggt gttgcggcca aagcatctgc
    30481 aattgctgaa tcgtttatta aaactgcatt ttcagcattt tcccaacctg cttgacttat
    30541 cttaatagaa gtttcatatc tatcagttcc tgtaagtgca cttgcattta cttcatcttg
    30601 atatgtaacc tcaaatctag catttggact tcctaagaaa acaattacgc ctccttttgg
    30661 caatctaact ctattgttct ttgtaactaa agaatagttt acaggaacac ctttatcatc
    30721 ataaacagca aaagatgaat tttgaggaag attaacttct atttttttat ttgcgagatc
    30781 gtcaccaatc ttataccatt ttgcataacc attggattct agttcacaag taaaagattt
    30841 ttctgctggc aaattagtta tagaatcttc actaacatac gttagtgttc caaaagataa
    30901 atactctgta ccattttctt tatgaagttt aatgtcggat aaatcacgac ctattacacc
    30961 aggtatttct ataaaagcat ttgagttatt ctcatccatt attttagtgt taactatata
    31021 acctggagtt tcatcagaaa gagcaagagt tgccttcaca gaaccaaaca tatagctttg
    31081 agaagtatat ttttcatcta ctagatagta atctttacca tttctttttt tccatgcttc
    31141 ttttacacta tttgatatat tgttgtcatc tatcttttga gctacatagt ataaacttgc
    31201 tgtctgacct aatccaggga catcatcata agagctcata tttaaatatg tgatattgtt
    31261 tttttctttt acaaatttta gacagctgtt acctttttca gatacaaatc tatcttgacc
    31321 tatgtataca tatttatctt ctggtccatt ttctatataa ggagaagata ctgttaaagt
    31381 accattatca ttaacatcta cttttatcat atttgaagaa gcataaagac cactattttc
    31441 ttttagagag ctaggcattt taacttgttg tggcttacta aatgttttat caggttttat
    31501 ttctttaatt ttaccttttt cttttaatgc agatagtaag atttcttgtc caattatttc
    31561 gttaagttga ctacttccac cagaagaaag aactgcaact gccatatttt catctggaag
    31621 aactataaga ttactgtgga ataataaaga gtctccacct tttgtaagag cttttaaatt
    31681 atactgatta aatggataag tgttgacaca atcccaccct aaaccatatc caagtataga
    31741 gtcttctcct tcaggccata gaccatttaa atattctttg ttttccatag cttttactga
    31801 agcaggagaa agaataccat ttgaattttt cataaatgtt tgtgcaaatg tacatagatt
    31861 ttctgcactt gaatataaac cacctgcacc tatagcatta aggttatctt gcggaactgc
    31921 atcttcccaa taaggcacgt atgcttttgc tagttttgaa ctatcaaaac tattttctgg
    31981 agtttttgta ttttgtaagt taagtggatt atttatgtat ttatcaagaa aattagtaaa
    32041 tgacatacca gatactcttt ctactaaaat ttcagctaaa gtaaatccat cattacaata
    32101 aacagaaaaa gctcctggtt tagcttttaa tctttgtttt tgtaattctt ttaagaaatt
    32161 atcatgacca taagaatcat tgtctgctaa taaaatagtg tttttaaaac tacttcccat
    32221 caaccctgat gaatgattta ataacattct tggagtgatt tccttgtatc tatcatcagc
    32281 cattttaaat tcaggtatat actttacaac aggagtatct aaatttaatt ttccatcatc
    32341 aaccaacttc ataacagctg ttgtagtaaa cattttactg atagatgcga tactatacat
    32401 attatcctta tttaagtttt tgttatcctg tttgctgtaa actccaccat taccagaaat
    32461 ctcaatttta ccattatcaa tcaaagcata ttgagcactt acagcaccat actttgatag
    32521 caaagtttgg attttctctt tagcaagagc tttagttttt gtgtaagatt ccgcgttttt
    32581 actataatca tgtaattcta tatcagaact ttgatatttt tcggaattat cttttgtatt
    32641 atcagcaaaa gtaatatttg gagcagcagt aaataacata ctcaatacta atgtactaga
    32701 taataattta gagagccttt gcttaaacat attacattac ctctttccct ttttaataat
    32761 ttataattag cataacattc ttaattattt taatcaacag attattatca ataaaaaaag
    32821 cttttataag tatagctttt tatataacag agaaaaatat aaaaataata tttgttgttg
    32881 tataaagaat aaagggtata ggtaatatat aaataaaatg gaggaatttt agacatatgt
    32941 ataagaatat aaataagaag caacttaaag aaatgatgaa aaatgaaaag gatgttttat
    33001 tattagatgt aagaactaaa gatgaattta aggaatataa aatagaaaat gctataaata
    33061 tatctttaca agagttaatc aataatatag atgaaattta cgattataaa gataagaaag
    33121 tagtagttta ttgcagaagt ggtcatagaa gtgttactgc atgtaatttg ttagctgaag
    33181 aaggttttga gcatttatat aatttaaatt caggaataat tgattatatg agttaaataa
    33241 tgtaagttat acattaaata ctatgtatag ctttgcttat taagtaggaa tatatctaaa
    33301 tatttataag ataaatttag gaaagaacga attttttcgt ttttttctat gacagttttt
    33361 gtagtaaata ttagttttta agttggaaaa aggtagtagc tttttagcta ctacctttta
    33421 atatgaactt agaatattat taaaaaaata tatttaaaga cagaattaat tttttaatga
    33481 ccagttaaat tactatcacc aaatttatca ttaaatggtt ttataactct atttataggt
    33541 ccttttagaa gtacatttaa acaaatagac agaatcataa atagtaatag aactagtata
    33601 tctttagtta gatttggagc atatatacca ccaactgctt cccttgtagc acctattgca
    33661 taggtaaatg gcattatagg gtttattctt tggaagaatg taggtgtaac ttgtataggg
    33721 aatgtaccac cagaagaacc aatctgaacg actaatagta ctattgcaat tgcttttcct
    33781 acatttccaa atacagaaac taaagaatac actataaagt taaataccat actagtaaac
    33841 ataagcagaa ctatcaataa aattggatgt tttgctgtta ctcctaacaa taatatatct
    33901 ccagttccta ttataaaacc ttggacaaga gctatactta aaaaagtaag gcctcttcca
    33961 aagtaaattt cataaggttt atagtttcca tgtacagatg tagacaatag tgctgaaata
    34021 agtaatatac caacccatgt agaaagaaca ctataaaatg gtgtcattgc agaaccataa
    34081 ttttcaattg gatataattt ctctgttttc acctcaacag gttcttttaa gaaattagac
    34141 tttgtcaata tatcactttc aagcaagctg actaattttt tcatatcttc accattacta
    34201 attttgctta aagtatcaat caaatcatcc aacattcctt ttgctaaagg aagtctttct
    34261 cttatcaatg atatactttc ctgtgcattt cctgaaagtt ttagagaagt agtaagtatt
    34321 tcttcaacct ttggaagttt tgcttctgcc ttatctaaaa cagtaattat atcatttgct
    34381 acttttatac tgtttgcaaa tatactattt ataggttttg atatttttga gtcaaaatta
    34441 tttaaaatat ttaaattaat tcttccaata ccatttgata gacttaaaac attattcaaa
    34501 gcacttatag atggttgttg tccagatata actttgtttt taatatcatt taatgttgat
    34561 atagatgaat ctattttatt gctagagtct tcaagattat ctattacatc atccaatcta
    34621 ttatttgatg taagttggtt taatttagtt aaaaagtcca ccaaagtatc gtttaaagat
    34681 tgcaaattag aaagtttttc tgataaatta tctatcaact taggtacatc ttcactgcca
    34741 ctatttactg catcaattaa atttaatgta agagaagaag cagaagatga aaggtcaacc
    34801 attaaattta agtcagactt aataagagga gaaagttcgt ctaaattatc atttgtatcc
    34861 tctaagaatt tttttaagtc acttgataat agttttgtat cattcagagt ctttttaatt
    34921 gtaggtaaat cagatttaat atctttaact gtatcagata attttgaagt agcatcagaa
    34981 gctaaatcaa cagtcttatt tatatggtca aatctctttt gaaggtcaat aagggaattt
    35041 tcaacttttg tcaattttgg aagactgtgt tcaattcctg caccaactcc gtttgatata
    35101 ccaagaacga tttcactaac tgtttttact atagtctgat taacctgtag ttgtattgta
    35161 gaagcacctt tatctgtaat ctttggtgca actgcattta ttttttcatt aacagtatag
    35221 ataatttcgc cttttttaac atcatctgta attaaagatg tcaaatcttt tgtaaaattt
    35281 tttggaatct taagtgatgc ataataagta ccttttttaa caccttcaag agcagttttt
    35341 tcatctacaa actgccaacc taaactcttg ttatctttga gttgttcaat taattggtct
    35401 cctacattaa tcttttgtcc tgctatttca tttcctttat cttcatttgt tactgctaca
    35461 agcatattag cagtgcttcc ataagggtcc caagatgcct ttatattgaa ccaagcatac
    35521 agagatggta aaaaagcaag acctaaaact attacaagta cggcataatt gccacgtata
    35581 tcttttaggt cacttttaaa aatttctaat atatttttta aaccctttga aggtcttaaa
    35641 ttcttgatat tattccagtt taaaatttct tttaaactat tagttttttt caaatgtctc
    35701 acctcctaaa atattttgat aaagaaaaaa gttagattta taatcaattt taaattatat
    35761 tactttttac acaaatagta aagttaaaat t
//
LOCUS       DAGSWI010000027        35487 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.027, whole genome shotgun sequence.
ACCESSION   DAGSWI010000027 DAGSWI010000000
VERSION     DAGSWI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35487
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.027"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(290..787)
                     /locus_tag="MJF80_001214"
     CDS             complement(290..787)
                     /locus_tag="MJF80_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBZ0279886.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFKFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            complement(962..1873)
                     /locus_tag="MJF80_001215"
     CDS             complement(962..1873)
                     /locus_tag="MJF80_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBZ0279887.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            2843..3694
                     /locus_tag="MJF80_001216"
     CDS             2843..3694
                     /locus_tag="MJF80_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBZ0279888.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            3698..4168
                     /locus_tag="MJF80_001217"
     CDS             3698..4168
                     /locus_tag="MJF80_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279889.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            4181..5491
                     /locus_tag="MJF80_001218"
     CDS             4181..5491
                     /locus_tag="MJF80_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0279890.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            5703..6449
                     /locus_tag="MJF80_001219"
     CDS             5703..6449
                     /locus_tag="MJF80_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBZ0279891.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            6811..8352
                     /locus_tag="MJF80_001220"
     CDS             6811..8352
                     /locus_tag="MJF80_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBZ0279892.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            8387..9493
                     /locus_tag="MJF80_001221"
     CDS             8387..9493
                     /locus_tag="MJF80_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBZ0279893.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGKKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(9703..10989)
                     /locus_tag="MJF80_001222"
     CDS             complement(9703..10989)
                     /locus_tag="MJF80_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBZ0279894.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(11112..14210)
                     /locus_tag="MJF80_001223"
     CDS             complement(11112..14210)
                     /locus_tag="MJF80_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBZ0279895.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            14935..15093
                     /locus_tag="MJF80_001224"
     CDS             14935..15093
                     /locus_tag="MJF80_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279896.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(15380..16084)
                     /locus_tag="MJF80_001225"
     CDS             complement(15380..16084)
                     /locus_tag="MJF80_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0279897.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(16075..17682)
                     /locus_tag="MJF80_001226"
     CDS             complement(16075..17682)
                     /locus_tag="MJF80_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBZ0279898.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(17936..19114)
                     /locus_tag="MJF80_001227"
     CDS             complement(17936..19114)
                     /locus_tag="MJF80_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279899.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(19340..19834)
                     /locus_tag="MJF80_001228"
     CDS             complement(19340..19834)
                     /locus_tag="MJF80_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBZ0279900.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(19835..20596)
                     /locus_tag="MJF80_001229"
     CDS             complement(19835..20596)
                     /locus_tag="MJF80_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBZ0279901.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            21318..24152
                     /locus_tag="MJF80_001230"
     CDS             21318..24152
                     /locus_tag="MJF80_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0279902.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFSETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            24195..25439
                     /locus_tag="MJF80_001231"
     CDS             24195..25439
                     /locus_tag="MJF80_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBZ0279903.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(25854..27596)
                     /locus_tag="MJF80_001232"
     CDS             complement(25854..27596)
                     /locus_tag="MJF80_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279904.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(27580..28254)
                     /locus_tag="MJF80_001233"
     CDS             complement(27580..28254)
                     /locus_tag="MJF80_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0279905.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            complement(28291..29280)
                     /locus_tag="MJF80_001234"
     CDS             complement(28291..29280)
                     /locus_tag="MJF80_001234"
                     /inference="COORDINATES: protein motif:HMM:NF012311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBZ0279906.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            complement(29328..30572)
                     /locus_tag="MJF80_001235"
     CDS             complement(29328..30572)
                     /locus_tag="MJF80_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBZ0279907.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            complement(30614..33160)
                     /locus_tag="MJF80_001236"
     CDS             complement(30614..33160)
                     /locus_tag="MJF80_001236"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0279908.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            complement(33368..33529)
                     /locus_tag="MJF80_001237"
     CDS             complement(33368..33529)
                     /locus_tag="MJF80_001237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279909.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            complement(33585..34601)
                     /locus_tag="MJF80_001238"
     CDS             complement(33585..34601)
                     /locus_tag="MJF80_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBZ0279910.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPDID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            complement(34598..>35487)
                     /locus_tag="MJF80_001239"
     CDS             complement(34598..>35487)
                     /locus_tag="MJF80_001239"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBZ0279911.1"
                     /translation="ISVLNTPHIIITGGEPLIREDFFEIIKYIKQKLPDTKLILSTNG
                     TLIDEHNVDFIITNFEKIDISIDGVDENTCSETRGKGVFNKVISSVNLLKDNGFSNIK
                     LSMVFGQKNSHLNKQFKDLNKKLGTEFVERYFIPTGRGLENKESYLIEDTVLPITIPY
                     ILDNELKMCKDKKNKKMGCCACNAGNSQIFINYDGDVYPCPSLIKDDYKMGNIFDNEF
                     VKVIKENKTRDLKSLIKLESIYPYNFEKCKDCDVNIFCWHCPAILDSVKDNELELDRW
                     CSMMKHNLNTAVWGDEKVN"
BASE COUNT        12193 a         5646 c         4031 g        13617 t
ORIGIN      
        1 atatttttca aattgtatac tcataaatcc agaagtattc taatgtgttt ataatacttt
       61 ttttcatatt ttaaattaaa taacaaatca ttatatttac aagtatcatt tatcaataaa
      121 caatattaaa tgaagaatat gtctgaagat tcaaagtgat actgatacta ttaattacta
      181 aaaaagcagt aaatctttta cagatttact gcttatagtt ataaataata aaactatcta
      241 taataaatgt actatactca atacttcatt caatatagta ataacaaatc tactttattc
      301 ctatgtaaat atgaacttct tgtttctttg tatcaaaagt attacattga tactcttcaa
      361 aatcacttat ataagtccta tcaaaatcca tttgccaaaa cttaaaccaa aaatctccta
      421 cttctttttg acctcctact ataacaaact ttgcatactt acctgctggt ataactttac
      481 taataatact ttctccaaca gtattattag tgtttaattc ttctaaattc ttctctttat
      541 cagaattact atttacttca caacatgcaa caaagctata cggacttgta aaatcaccct
      601 ggtaatctgt atatagacct attgtcttgt cattcttttt accctttata gcattatata
      661 tccctttgcc cataaatttt ttccacaatt cacctatatc atttaccgct tgtccatctt
      721 tatttgtagt ctccttcgat attcctataa ctattttctc atttatctct actatttcat
      781 aattcatact ttatacctca ctttgttttt attaatatat tttattttga actaattcaa
      841 ttataattta taatatacga cacctttatg tcatattttt aatataatta aactcagtga
      901 gatattatat actaaatgca agatattata tatttaataa aatctttaat ctagcattat
      961 attataatga ataaatttta gacaactccc ttagtctagt tattacatta tccctaacat
     1021 ctttaggttc aagtacttca acattgtcac caaacgataa aatatatcca taaatccatt
     1081 caccaatagg aaattctgta attacatcat atgtatcatc ttcattttta gttattaaat
     1141 ccttattaaa atcatcaaat actctaaaaa gcatcttttc tttaaacctc aatttcaacg
     1201 ttatagtatt ttccttaatt actttagaat cattcaaaca tacttcttct attatctttc
     1261 ttgaaaacac ttcatccttt atagataaat tccttatcct actaattcta aatatcctaa
     1321 aatcatcttt tagtttacaa aatccataca agtaccaagt cttactctta tacataagct
     1381 tcagtggttc gacagttctg tttgtctggc ttgacaaact atttacataa ttaaaattta
     1441 tagtattacg ccttaaaata gcttctttta tttttgtaaa tttatcatct tcattaaaat
     1501 tacttcccca ttcagtaaag tctatttcaa tccagtttga aaaattctgc tctccaaata
     1561 ttgagccaat tttatttact actgaattta tttctggata cttagtcgcc tgtaatgttt
     1621 tcagtgcaac tattaaactc tctttatctt tttttgacaa tatagccttg tctatagaat
     1681 attcttctat taaagatatc cctcctcctt tgcctttcgt catatatact ggtactcccg
     1741 acatagacag agtttctata tctctatata tggttcttgt agaaacttca aacctatctg
     1801 ccaactcttt agctgttact aattttttat ttaatagaat tactattatt tctgttaatc
     1861 tatttatttt cattcattac cacctactaa aattattaaa tttatttaat ctaaattaat
     1921 catactaatt tttaaataaa caaaatctta gaatcttatc taaattattc ttacatgatt
     1981 ttatttactt tctattatct ttctcacttt ggatattatc atcttaattt atttattact
     2041 atatcaattt acttattatc atctcatttg ttatcgtctt acttaattta cttattgcta
     2101 tatcaattcc acttattatc atcttattta acttacttat ttctatatca attcacttat
     2161 tattacctca cttgttatca tcttacttaa tttacttatt tctatatcaa tttacttatt
     2221 atcatctcat ttgttatcgt cttacttaat ttacttattg ctatgtcaat tcatttatta
     2281 ttatctcact tgttatcgtc ttacttaatt tacttatttc tatatcaatt cacttattat
     2341 catcttaatt catttgatta ttatcattta ctttattcaa tttctatatc aaaaattagt
     2401 atttgtactt aatacttaaa attaatttaa agtctttaca tattatttat tttttattgt
     2461 aatattcatt tccatacttt actaagctac ttccactcat ttattttaaa atttcaattt
     2521 actatttctc aattctatat tattattttt caattttata ttactaattt taaagctttt
     2581 tagctattat tcctacaata tttaaacaat tttttcataa tgaagctata ttacaatata
     2641 ttcaaaatat gtaatatttt agatattttt cgttgacaat gttaaatttt tgttttatcc
     2701 ttaattaagg acatgttatt gacatttctg aaacttattt gaaatagttt tttcatttta
     2761 tttaggaaaa cgtattatac ttaagaaaac gtattttctc atttcagata agtaaaatat
     2821 ttattctttt gaggaggaaa tcatggaaat taataaaaaa cattttattc aagtattttt
     2881 aacttgctta atcttggtag taatatgtga atttataggg caaagaacta tagctattgg
     2941 aaaagcctct atagtactgt tccctatgct ttatgcagtt ataattggac ttataataac
     3001 accagattta ttaggtaaaa aaattaaagc acttaaaaaa gtggttggag aaaaagaaat
     3061 aaatattgct ggtgaagttg ttggaattgc actagtaatg cttggtataa aatatggtac
     3121 tacagttgga ccaaacattg acaaaataat tcaagctgga ccagcgttta tcgctcaaga
     3181 atttggtcat atacttgcac caatcgttgc attacctctt gcattaatgt ttggaatgaa
     3241 aagagaagct gttggagcta ctgcaagtat aagtcgtgaa ccttctttag gagttatatc
     3301 agaaaaatat gggataaact caccagaagg tagtggtgta cttggtacat atttaatggg
     3361 aacagttctt ggtactatat actttagtgt actaggttca atatctattt atagtggtct
     3421 tcacccatat gctttgggaa tggcctgtgg tgttggtagt ggaagtatga tgacagcagc
     3481 tgctggttca ttatctacca tggtaccacc tgaaatggca gatacaatac ttgcctatgc
     3541 tgcaacaagc aatatgatgt ctagtataac aggaattata ttcttagttt ttgtatcctt
     3601 accatttact aattttatgt ataagatttt agaacctaaa ttttcgagaa ataaaaaaac
     3661 taaagaaatt aaatctacag aaggagagtg ctaataaatg tttgctaatt taaatttaaa
     3721 gaaaagttca tatcaattat tggttatgtc tgttattgga gttataattc ttataggaca
     3781 aagaattagc gttggtacat ctatagttac agctcttcct ggtatggtga tgctaatatt
     3841 agctgctatg gcagctatga ttataaaaga tttatttcct aaatcaatct ttccagcttt
     3901 tggatttgca actataatcg gccttttact tagtatgcca tatagtccaa catctgaagt
     3961 attcttaact aacactaata atattaactt tatggctata actactccac tactagcttt
     4021 tgcaggaatt tctgttggaa acaaaataga agctttaaaa gaaatgtcat ggaaaatagt
     4081 tataatttca cttattgtat ttacaactat attctttgca tgtgcgagta tagctcatat
     4141 agttttaagt attcaaggta aaatatagga ggaatttatt atgaataaag aacaattaaa
     4201 atctctagta ataaaaacta tagatgaaaa tagagaaaaa atattaaaaa taggtcaagg
     4261 aatatacaaa aatccagaat atggatataa agaatttaaa actacagaag ctgtttcaaa
     4321 cttttttgag caagaattaa atttatctgt agaaaaaaat atagcagtta caggatgcaa
     4381 agcagatgca aattctagca agaaaggtcc tcatatatct atacttggag aattggatgg
     4441 tatatcatgt aaagaacata aagatgctaa tgaaataggt gcttctcata cttgtggaca
     4501 taatattcaa atagctggaa tgttaggtgc tgctgttggt ttagtaaaaa gtggcgtttt
     4561 agaacattta gatggtaaag tatcttttat ggctactcct gctgaagagt ttattgagtt
     4621 ggaatacaga gagcaattaa aaaaagaagg aaaaataact tattttggtg gaaaacaaga
     4681 attaataaaa agaggttatt ttgatgatat agacatatct atgatgtttc atgcttctga
     4741 tttaggagaa gataaggctc tagttggtcc tgagagtaat ggatttgttg gaaaaaaggt
     4801 taaatttata ggtaaagaat ctcatgctgg ttctgctcca catgatggaa taaatgcttt
     4861 aaatgcagca atgcttgcta taaacaacgt gaatgcactt agagaaactt ttaaagagtc
     4921 tgaaagagtt agattccacc ctatcatcac aaaaggtgga gatatagtaa atgttgttcc
     4981 tgctgatgtt catatggaat catatgttag agctagaact ataaatggta tgattgatgc
     5041 taatgaaaga ataaataagg ctttgatggc tggtggaatg gcagttggag ctgatgttga
     5101 gataactgaa attcctggat atcttccaat attacgatac aaaagtcttg ataatttatt
     5161 taaaaacaat cttgaagatt taggtctaaa aggaaaagtt attgatggtg gagattttac
     5221 aggctctttt gattttggag atgtgtctca tataatgcct actcttcacc ctatgattgg
     5281 tggtgtaaaa ggagctcttc atacaagaga ttttgaaata gttgatgaag atttagctta
     5341 cataattcct gctaaagcaa tggcacttac agttgtagac cttctttttg ataatgctaa
     5401 ggatgctaaa gatatattag atacctttac tccagttatg actaaagaag aatatctaac
     5461 attcttagaa aaacacgata aaactttata agtctatttt tataataggg tggtatctac
     5521 tgtaaaagtt ataccactct ttgtttttgt aacagtatac ttcttttctt ttagttttta
     5581 gattaaacta aaatatatta tcctccttaa aacttttaga gctacgttga aatatcagta
     5641 gaactatgct atattctttc taattaatat aaatttctat tctaattcaa ggtggtgaaa
     5701 aaatgtttaa tgcaggaaat cgaattataa atacatggtt gtaccctatt caagatggct
     5761 ttgtcttgat tgatactgga tatgaaaatg gttttaaaca tttcaaaaaa cgaatttcta
     5821 aattcaacat aaaaatcaaa gatattcgat acatatttct aactcatgct catgatgacc
     5881 atgttggttt tctaaatcag attctagacg aatctcctaa tacaaagatt gttatgcatg
     5941 ataaagcttt agaaattcta tataaaggtc aaaactcttt tattggtggt tgtaccagcc
     6001 gtactgcctt tctcttttgc caaattatga aactatttgg aaaaggtgag catagttttt
     6061 ctccacttaa acaagaattt gaaaaaaggt gtattcttgt gtctgacaag aatcgtaaag
     6121 aaattgaaaa agaactaaat ggaaaaatta tagatacacc aggacatact gatgattcaa
     6181 tttcactact aattgatgaa ggtgttttat tttgtggaga ttctgcaatg aatggatttc
     6241 caagtactca caaaattacc atatttgcag aaagcaaact agaatttatt caatcttgga
     6301 aaacaatcat caatacaaaa cctaaaatga tttatcctgg acatggaaaa ccatttgaat
     6361 acagtaaatt agaacaaaat ctaatctatg taaataaaat aaatttatat cctctacata
     6421 attcaaaaga tattcacaga tttttgtaga aaatacaaag taaatttatt tgattaatta
     6481 aaaaatctaa cactttacta cgctacttat tttaataact taatcttcca ctagcttttc
     6541 ataaaatttt gttttaaaat taattttaaa gtgcatatgt tatttaaata cattagataa
     6601 aattcgtcat taatctactt aaagcatatt taactaacac cactcaacca gaaaattcag
     6661 aaaactgcat tgacacagga aaaagtttat tttatacttt ttttaggaca tattgtggac
     6721 acgtcttaaa tcgacaaaca gaatttaaat tgtaagatta accacaatac ttatttttta
     6781 attatatttt taaacattag ggaggatatt atgaatgata acgtagttaa gaagaaaaac
     6841 ttttttgaca ggacattaaa cagaatagaa actgttggta ataaactacc agacccagtt
     6901 acaatatttt tagggttatg tgtactttta cttatactat ctagtctagt tggttctatg
     6961 ggtatatcag tagttcatcc tggtactgga gaaactataa ccgctgtaaa tttattgact
     7021 gtagaacagc ttcaaattct tttaggaaac atagtcagca atttccaagg gtttgctcct
     7081 ttgggtctag ttctagtaac tatgataggt gctggtgtat gtgacaaaac aggacttatg
     7141 acagcaacaa tcaaagcatc agtaagcaag attcctgaaa caagagtaac tcttgttgta
     7201 atgactatag gtatgcttgc caatatagct tcagatgcag gtacaatatt atttccacca
     7261 ttagctgcac ttgtatatct tggtgttggt agacatccac ttataggttt attttctgga
     7321 tatgcagcag tctgtctagg atttgccgca aacattatga taagtgtcaa tgatatactt
     7381 gctgcaagtt ttacagttcc agcagctcaa atgattgatt ctggatttca agccaatgca
     7441 acaatgaact tattttttat gatagcatca acttttgttc taatactttt ggcaacatgg
     7501 gtaacagaaa aaattgtagc tcctagattt ggtaaatatg aaggaaatgc agaactagat
     7561 gttgatggaa gtcttagtga tgtagaaaag aaagggctta aaaaagctag tataagtata
     7621 ttaatatatg tggctattat agtagctctt agtgtaatcg gtaaaaaacc attcttagct
     7681 gacccagaaa caggagattt actatcaaac aatgctcctc ttatgaaagg tatggtacct
     7741 ataatagcac tagcattttt catacctggt gttgtatacg gaaaaactat aggtaaaata
     7801 aagaaggata aggatgtagt ctctataatg gctagtgcaa tgagtgatat gggtggatat
     7861 ataatactag catttgctgc ttcacaattt ttgcaattat tcacaaatag taatttaggt
     7921 ctgatactag ctgtaaaggg cggagaattt ttaaaatcag caggaataaa tggaccttta
     7981 ttgttgatag gatttattat attatcatgt tttataaatt tatttatagg tagtgcatct
     8041 gcaaagtggg ctattcttgc acctatattt gtaccaatgt ttatgatggt tggatacaat
     8101 ccagcactta ctcagatggc ttatcgtata ggtgattgta taacaaatcc acttagccca
     8161 ttatttccgt acttccctat tatactagca ttcacaagaa aatacgataa agatgctggt
     8221 atgggtactg taattgctaa tatgatacca tactctatat cattcttaat agtgtggact
     8281 atactattaa tattgtttat ggtatttaat attccactag gacctggaat acttccttca
     8341 tatagcatgt aatttattta aatctttcta aaaggagaaa taatttatgt tagataaaaa
     8401 tagacttata aataatttta tggatatggt tagaattgat agcccatcta atcaagaatt
     8461 agaaatgtct aagtggttgg taaactactt aaaagaaaga aatatagacg ccattataga
     8521 tgatgcaggt aaaaaatatg gtggtaatac aggtaatgtc attgcctata taaaaggtga
     8581 agaaggaagt agaccacttt gtttatgtgc acacatggac caagttcaac cttgtttagg
     8641 tgtaaaacca atactagatg gtaatgtagt tagaagtgat ggaactacta cacttggtgc
     8701 agatgataaa gctggaatag ctgctatact tgaagcttta gaacatgtta ttacagaaaa
     8761 aataccacat agagatattt atctttgttt tacaatctgt gaggaagctg gtatgcatgg
     8821 cgtcaaaaac tttaatcctg acaacttgcc ttgtaaagat atggtgattt tagattctgg
     8881 tggagctatt ggttcgatag catacaaagc tcctgctcaa caatctataa aaatctcttt
     8941 tcatggaaag aaagcacatg ctggtataga acctgaaaaa gggttaaatg ctatattagt
     9001 agcctctcat gcaataagca acatgcacat aggcagaata gattcattga ctacttcaaa
     9061 tataggtaaa atagaaggtg gtggagctac aaacatagta actgataagg ttacattgac
     9121 tgctgaaatt aggtctcata tacctgaaac tttagagtat gagcttaatc atatggaaaa
     9181 atgttgtaag gatgctgcat ctaaatttaa tactacttat acttttgaac ataatatgtc
     9241 ttaccctagt tttgaactta gtagagatag tcacgttttc aagttaagtg aagaagcaat
     9301 aagacaagtt ggtgttgttc caaatccaat ggtaattggt ggtggaagtg acgcaaatat
     9361 attagctaat cttggatata attgtgctat attaagtcta ggtatgtatg atgtacacac
     9421 agttaatgaa tatgtaaata tagacgaatt atatgatact gccaaaatag tttatcatat
     9481 gattaaatta taaatatcat aatagaactc tttaaattat aaaaataaaa acctatgtat
     9541 cttcaacttt ataagttata tattagagtt gttttacata ggttttagta tttttataat
     9601 aatttgttct aatttttttc ataataagct ttaatacttt atatagtaat ttgttgtaga
     9661 attttataca atataatgat ttatacagtt ttataaaata tcttaaaaca gtatttcagt
     9721 aatatttttt ggtcttcctc caccaatact agtctctttt gcacatattc gacctaaacc
     9781 tgcatttact attttattta atatacgtct tgcacttctt tcacttacat ttagatactc
     9841 tgctaactct tttgaatcat aaatcttact ttttctattc tcatttatag caataatttt
     9901 tgcaactgac tcacaactca ttccagtctt tttagatatt tcgattacct tttcatcaga
     9961 tagcatcaaa gaataattta attcattttc ggaagctaaa ggtcctctta tgttttcatc
    10021 ttcatctacc aaatatatac caatttcttt tgaatctatg gagtgtctta atgctttatg
    10081 tgcattattt tcagcctgat acgcagttac tccagtacca attcctatac taagagaaaa
    10141 gcccatgtct tttatctctc tctttagatt gaatagctta tcataattat actcattatc
    10201 agctacccct ttatgaacaa atacaacata atcatttctt ccaagtaaaa ataaggaacc
    10261 ttgaatgctt cttacatagt ttactacaag cttatccata tctgattttt ttatcatgtc
    10321 agaataatag ttatctatct tctctttata atctattaaa ctcaatattt caacagcaat
    10381 ttgagaaaat cttgccttac ttagagcata ttcactcttt accttgtcat aactttcttt
    10441 gattaattgt atagtagctt gtaaacgaaa tacaggatat cctctttctt ttaattcatt
    10501 atatactgca ccaaaaccag tcaacattat atccgtctta ttttcttcaa ataattttat
    10561 atgccattct acaagttcag attctcctac ttccaaagaa aatggattac aaaatacact
    10621 cgttgcttta atgtcaaatt ctttaagtgc atcttccaca acatatctat caactacatc
    10681 tatactgaat ttatctaatt ttatatttgc actttttatt gcccaaaatg ccttcattat
    10741 actcgtccca cttctcggta caaatgaatg tggcttactt atctcatact tagattttat
    10801 aaattcataa actgcacatc ctgtaaatat aatagcatca cattcgtctt cacatttaga
    10861 aataacttct attgtatcta ctactttttc tcttacatat attttagtct ttaaatcact
    10921 gtctatctgt tttaaatcat cctttatctt aaacccagag tctgaaggac ctataatacc
    10981 cactatcata aacttcgctc ctaatccata aatattatac tttttaaaaa ttatatcctc
    11041 actattttct acttctattt aattattttg aactactcca tcctacctac atttagaatg
    11101 gaataattag cttatttttg ataaagtaac ttctcaaaat cagcacttgg aataggctta
    11161 caataataat aaccttgaat taccacacaa tctaaactca ctaaaaattc agcttgttct
    11221 tttgtctcta caccttctgc cactacatcc atatctaaat cttttgccat ggctatagtg
    11281 tgctttaaaa ctattttttc tctgtctgtg attccttcct cattaaagaa accacaatct
    11341 atctttaatg tatcaatatt aatatttttt aacatattta atgaagaata tgctgaacca
    11401 aagtcatcca tatttaaaac aataccatcc attctaaggt cggctattat cttagtaaat
    11461 aattttatat ttttaataaa taaactttct gtaagctcta attctaaaaa agattttggt
    11521 atactatatt tatttattaa acttaaaaca atttctttaa aatgtggatt atgcaaatgt
    11581 aatcttgata catttacaga gataggaact ggctcttcac ctaaatctat ccattttcta
    11641 atcaaagaac atacttgttc ccatacatac atatctaatc tgataataaa cccattcttc
    11701 tcaaatagtg aaataaattt aattggaggt attatacctt tttctggatg aacccatcta
    11761 accaatgctt ctgcaccaac aattttattt gcattaagat tatacttagg ttgtaaatat
    11821 acatggaatt gcccattttc aagtgcgtat tccatttccc tttccatctg tttttcttca
    11881 agtgcatcat ttcttatagt atcatcataa aatatatagt tgttgatact ttgtttcttt
    11941 acttttttgt gagcatatcc tgccatttca cacgcatttg taattggaat atgattatcc
    12001 aaaattctat aaactccaaa acatggtact aacatatatt caaatttttt tctatttaag
    12061 ctatttttaa tcttataaat taccttatga atatctctat cagagtaact ctcataaaaa
    12121 atgcaaaata catctgcata catacgacaa catataccat tagaaccaat gtttttttga
    12181 atcaactctg caacatatat taaagcttta tctcctcctc taactccata aatgtcatta
    12241 attaccttga acttatctat atccatcatt ataattgcat aacgcttgtc aggatatctt
    12301 ttaagcatta aacttatttg ttcacaaaaa ctttcataat tgtatagtcc tgttaatgta
    12361 tctttttggt ttttatgtag ttgcataaga taatctatat tttttatagc aactaaaata
    12421 tgtgtttgtt tctcacctaa cttattttta ctactaatct ttaacatata aattaatgcc
    12481 caatagtact ttttatctat tcctaaacgt ctatactcaa aatttagttc taaatcgtct
    12541 tgcttgctat tattaagact atcaagagat tttagaaggt ggttatcatt aaataatatt
    12601 atatcatcag gatgaattaa atgttttttt atgtatgaaa aagttttttc aagattttcc
    12661 tttataggaa aactaaaaat attgctctct gattgataaa ttggagtaat taaattcgta
    12721 tcaatgcaaa tagataaaat tatatcatat gtattataag ctaatttttc ataagcctta
    12781 gaataccctt ttatcattaa atcatcaaaa taaaccttat caactaactc tttatgttta
    12841 aataaaatat tattcgtcga atcagctaag tcactaatta ctttcatttc attatttcta
    12901 agtttgttct tattataata cctcttctta tcttggtaca ttctctcatc agcaacttta
    12961 attagcttat gtatatcaat attatcgtca ctccaaatac tgcccactgc tacactatat
    13021 atcatctcat ccttaaattc ttcacttaac caactcacta attcttcaaa tagttgtttt
    13081 gatatatctt cacataatac aacaaactca tctcctccaa tacgataaac attataagaa
    13141 tgaaagtatt tttttattat tacacatgct gacacaatag cagaatcgcc attagcatgt
    13201 ccaaagttat cattaatatc ttttaaccca tttatatcta caaatacaac tcctatggat
    13261 ttaacttttc ctttttgaaa attatctata tacttaatat atttatttct attatatgca
    13321 ccagtcaaga agtcatagta gctcatataa tttaatttat tttctacttt tctttttttc
    13381 atctcagtta ataaaaaata agatagggat gtcaaaagag aggtatctcc aacagcttta
    13441 tgtggattat caactccaat aaaccctact actttatcat cttgttttag tgaagatgtc
    13501 attaaacttt caattttttg aggttttaaa atttcatatt ctattggctg ttcttctttt
    13561 atattttcta tatcatcaat tattacaaaa cctttttgtt cgaataataa aaaccaattc
    13621 tttaattcat taatatcaag atttgttagt gcctgttttt gttcatcaac accttcattg
    13681 caccattcat atgtattagt tacttttgtt ctatcttctg atacttcaaa aacatatgct
    13741 ctatcagctt tatgaaattt accaatattg gttaatacaa gatttacagc atcagaaaaa
    13801 ttagattctg ttataagatg tttaatacat ccaactaata actcctttat ctctaatttt
    13861 tctgctattg acttacttat atcttccttt tctgtaatat caagagctat ttctaatcgt
    13921 gcatgtttgc cctcccattt aattaacttg tcctttaata taaaatgtct atttgttcga
    13981 gtattcgtat tttcccatat gtaatattca tcttctttta aaatagagtt tgtacaaaat
    14041 tcgcagggag aatctttccc ctgtaatgct ttataacatt ttaaatggga tatatcatta
    14101 agtcccattg cctttttccc aatttcatta atatataaaa gttcatatgt atctaaagaa
    14161 gaaacatata caatttcatc caactcatct attaaattta ttacatccat ttatatatat
    14221 ctccttttat tcattttcca gattgaattt ttaaagcttt taaaattttg ctatatattt
    14281 ggcaatgata ttatacttct ttatgtttta aaatagtacc ttaatgaggt tctatttttt
    14341 agtaaataga attattattg tgtcaatcgt acaatagagt tttgtaatac ttaatttaat
    14401 atatttataa ttaactatct tattatgtat ttacaattaa ttctcatata aatgacaaat
    14461 ctccacctca attaagtatt ccaatcatat atgaggataa tacttcattc atttaaaaat
    14521 ataaaactat tatatattaa aatttcttca ataataaatt atttagattg ttactttgat
    14581 atattatata atatcacaaa ggctgatata aaaagacaaa aagggcaatt tttttatatc
    14641 aaaaaaaatg ttaaattgat attatccata tataaattga tattatccat atagatgcta
    14701 tatttaacaa ttctacttac ttcccttaca cttattagca tatagttatt aagagtttgt
    14761 tgcaaaacaa cacttagcac actttaaaaa gtatggtcta ataagtaatc aacgaagaag
    14821 acataataaa gcataaaata tgatatactt ttccacgcca tcaaaactga ggattttatt
    14881 attaatatat gctaaaatat gcttaataaa ttaaatttta atgggagaat agttatgaaa
    14941 atatttagtt tcttgttgtt tgtgatagga atatgtttat gtattgctgg atttatagga
    15001 cattacccag tatttgttgg tacaacattg agttttcttg caattgcaat gatagctgta
    15061 agacaagtca aaactaagaa aaaaatgaaa taggcaaatc ctaactttac aagttaatat
    15121 tacacaagta attagtctaa aattggatac tttttctatt acagaaatac atcatccact
    15181 ttgtattttc ttaaaaaaat aaaatagcta tgtaaattca agattagata gattaactct
    15241 ctatccattt cattgtaaaa tatacatagc tatttgtatt cgatttaaaa tacttagatt
    15301 ttcttacata acaaataatt tctatctgat tattactcat attcatttag taatcactca
    15361 gcaactaatc atttagtatc tattcatccg ccatacggta acctacccca acctcagtca
    15421 aaatatattc tggctgagca ggattctttt caattttcct tcttatactt gccataaaaa
    15481 ctctaagcga ctgattttca ctttcatagt aatttcccca aactttatct attataaact
    15541 tatgggttaa gactcttcct gaatacttag acatcaactc aataatctta tattcgattg
    15601 gagttaaatg tatttcttca ccattaattg acacaatatg attttcataa tctatcttta
    15661 ggtttttaac ttcaaaagac tttggagcat cattatttat attatcctgt tgagctttat
    15721 ttctaagagc cacacgcact ctagccaaaa gctctgttac actaaaaggt ttagttaaat
    15781 aatcgtctgc tcctccatct aaagcttcca ccttatccct ctcatgctct ctcgctgaaa
    15841 caacaattat tttattagtc tttgcacatg ccctaactcg tcctattacc tcaatcccat
    15901 caatatctgg tagtcctaaa tccaaaatta tcaaatctgg gtcaagagaa tgtataagtg
    15961 atatagctgt tcctccatta tctgtctcta cacacctata attttgagtt tcaagagata
    16021 cttttataaa tttacaaatt ggcttatcat cttctacaat tagaactaat ggctttattt
    16081 ccacttgtct ttcccctcct catcatacat aggtatgcta aatctaaatg tagctcctcc
    16141 agaaggatta ttaaaggcga caatttctcc accatgagct tctactatag atttacatat
    16201 ctccaatcca agaccaatac ctcttctttc aagatttcta gacttattct tagtataaaa
    16261 cctattaaaa atattcccga tatcttcatt tggtattcct tttccattat ctgcaacttc
    16321 aaatagtact tgatttgact ctttttttac agttagttct atcttagaat ttttaggtgt
    16381 atgccttata gcattatcaa ttagatttac taacacttgt tctattaata atacatccac
    16441 atgaacgatt attatctcat ccggaatatt tgtttttatg tctctactat tggcaaactt
    16501 tttgactctt aatatagatt cagctattat ttcatcaaca acctctggtc gtttttcaat
    16561 atccaatttt ccttcatcta ttctggtcat actaagtata ttctcaacag aatgagttag
    16621 ccaactcgta tcttcatata tatttttcaa taattctact ctagtttctt tatctatcac
    16681 atcatcattt tcaagtattg ttgaagatga acctaatata cttgtaagtg gagttcttaa
    16741 atcatgagaa attgacctaa gcaagtttcc tcttaatctc tctctctctg cctcaagatt
    16801 tgctttttta tttttctcaa ataaattttc tctatcaatt gccaacgcaa cctgtgttga
    16861 aactgcttct aatagtatct tttcattttc tgtaataata tctatcttaa aacacgcaac
    16921 acctataaca ccaagaacat ttgattcttg accttttata gggaaataat atgcactgct
    16981 gtttttacaa tattttgtcc caattccaac tgggttacct gtcctaaaag cttcttttat
    17041 agcttccttt tctaatactg aatggaatat attctcactt ccatcattgt tacaaatata
    17101 aatacttggc ttacctagat tattattctc atctgcaata gtcactatta cagttctatt
    17161 taatatattt actaaattag acccacaaaa ttcaataatt tgatttttat tttttgcttt
    17221 aagcaacgtt ttgttattct catagagcaa tcttatcatt ttttctctct tatgagaaag
    17281 tctcacttct ctttttatcc tagaagttaa agtacttgtt ataattgctg ccattagcat
    17341 aatacaaaag gtcataggat aatcttttct ataagctaaa aaagtatata atggatgcgt
    17401 aaagaaaaaa ttaaatgata gcactcctat tactgaagaa agtattccaa aaaaatatcc
    17461 ataggttata cttgtagcaa atagtacacc taatatgtat actagtataa tattagattc
    17521 attaaaacct accttttcaa aaactactga tattccagta gcaattgtca taaccaatat
    17581 agtttttatg aagtccttaa taattatctt tttactgaga tagccatctt catcataatc
    17641 atactctttt aagtcttcat gttgtagtcg ttcctttttc atttactaca attcctcctt
    17701 attgttactg tttaaattgg ctctatttta tataatacaa ttttatcata ataaattcaa
    17761 tcaatacatt taattaatgt acttaaatta ctaaaaggtg ctgtcttaaa atgaaaataa
    17821 atctctattt caaaacagca cctatatatt taaattacta atttagtttt tacatactta
    17881 aatcaatagt tttattcaat ttcataaatt ataatacttc aattataaaa catttctatc
    17941 caacaagcat aaatataaag tgggcacata tacctgcaca taaaccacct atagtatgcg
    18001 aaagtatcgc tttaccagcc aatggtcttg catctaaagc atccatcata cctatgtgtg
    18061 tacttaaata tccactccaa cacataccca ttgctgtgaa tactgctata tcattaggag
    18121 ttatagcgcc tgattttata aactctggta ctaaagatat tgctgctcca acagctccta
    18181 atgcagttac agggaatgct attgcctctg gtgatgtgaa tccaaataat ggttctatta
    18241 tgaagctaat cttatcacca atagcaggta ataattttat accttcatat gcagctcctg
    18301 tgtaaacagc ttgacctgta gcagggtctg tagatggtcc aaaagttaat aacataacta
    18361 atgtacaaac tactaaaact cctggtatta tagccatacc catttcaaca cctaacttac
    18421 caccttcaag tatagcatcc aatgttcttt ggaatacatt accatctctt atttctctaa
    18481 actcttcaag ttttgcacct ttatcattta tttgcttttc agtatcattt ttagggtcat
    18541 aattataata ctttttagta aaagtcaaca taattctaac actaataata ctacctatta
    18601 ttgccgcaac attacctatt atagctggta gtacatattc tttaccttga gctatcataa
    18661 aagttgttac aataagtccc ataccaaagg ctgttcctaa attacataaa gcaggtactt
    18721 gatacttctt aaaatattgt gtaaatgttt tgtctttagc aaatggtata atagctggat
    18781 tatcagataa atatgttgca actactcctg ctatactagc accaggtaag ccccatatag
    18841 gtctcataag acccttaaat atcttattaa ctaaagatat tactccaaac tctgaaagca
    18901 atgcactaag tgcacctgca agaactgcca tagccattat taaaaatact gtgtctaata
    18961 aaagcgcatg tgctgtactc attattactt taaacatcat tcctgcaccc atgatgcttc
    19021 ctacatatcc aaatcctacc gccaatagta caataaatac gaaggtttcc atacttactg
    19081 ccttgactct tctcttatcc gacagctttt ccatcttgaa atcttccccc ttttaatttt
    19141 aaattaaggt tcctaacatt ggtacaaatt ttttggtaca aactgagttc taaaacttac
    19201 actcgtaatg caagttgtca gttttttgaa ataaaaaaaa attacgttta tagaataaga
    19261 atatcctctt taatttggat actcttattc tattttatgt tattatatta tcatcctttt
    19321 gtttaaaagt caattttttc tatttttgtt gtttaatttt atttatactc tttttatatt
    19381 tttcattttc ttttatcttt gtaagtactc catataagtt gacttttcta aatacagtag
    19441 ttataaatac caatcctaat gcaattgaac ttgcacacga taatgtactc accccatatt
    19501 ctacagcttt tatataatta ttggtcaaaa cagaatacat agtattatat attccataac
    19561 caggtactaa tattattaaa cagcaaacac ttaatgtagt tgctggagtc atataaattc
    19621 ttgcacataa ctcacaatat atcgcaaaac aaactgtagc tataaaaaaa cataagtttt
    19681 ctgttactcc catatttaaa agaaatttat acgaaaacca agctatactt ccacctattc
    19741 ctgctagtat taagtttttt cctttcatat taaatagcac tccaaagctt aaggcagtaa
    19801 aaaaagctgc tattacttct ataaacaaat gcacttatat cccccctaac ttgaagtaca
    19861 atccaagtac caagaacatt cctactgcta tagatacacc tacaaaaaat acttctgcta
    19921 attttgcaag accagaaatc aactgtccat ctaacaaatc tcttaatgca ttagtcaaag
    19981 caagcccggg aactaagagc attattgctc ctgctattaa atggtctaac gtctttatga
    20041 atccgagctt taaaattaaa attgaacaag aagctataat aactgctcca attgcattta
    20101 caaaaaagtc attaagggaa gatttactta aatacataat aacaaactta gtcagaatcc
    20161 caacagtaaa tgcagctata aattcttcca catttcctcc aaataatatc gaaaaagttg
    20221 ctgctgcaat acctgcaaaa aatattgtta ttaaatccga gtaatagtta tcttcactta
    20281 tttcatctaa aattttctca cataattcaa tatctaaatt atttatactt attgctcttg
    20341 ataatgagtt aacttcatga accaagttta ggtctactct tcttgagctt attcttttaa
    20401 ttgctgaatg taatttccca tctacaaata cagatgccat tacagctgtt ggagttgcaa
    20461 atacatctac atggtctatt ccaaaagatt gacatattct agctatcgtt tcttcaactc
    20521 tataggtttc tcctccactt tgcaacatag ctttacctgc atttgaggaa aagtttaaaa
    20581 ttctatctat atccacaaaa catatctccc attcttttat agtattattc atattttaat
    20641 acaagatatt atttatttaa actattttaa ataattcata tccaatattt acattttatg
    20701 aaaaaacttg ttttttatgc ttgtcaaaat caaattttaa gcttgtttac accataaaat
    20761 ggctgaatta atatttgaca aacactttca accatttata taaccttcta aattgtataa
    20821 ctttttacta caaataaaat tatatctctt ttaattcttt ctcttgagta tttagcttat
    20881 agtaacttca tatttatata tgtacaatat aaaacattat ttttatgata caaaatataa
    20941 tttgtaaatg tatatatgta ttaactgtgt atatataaat tattcttaac cttatcagtt
    21001 ttttcatata tcttcatcta taccgaccat atattaatta cattgaccat atattaataa
    21061 atgatattac atagacttaa gtatattaaa aaacttaaat ttagttattt aacaacataa
    21121 tactctaatt taatctataa aaatccatac ctaatataat gcaactctta ttttaacacc
    21181 atctattaca caaaacaaca tattaaaaag taaaatacaa aatatccact ataaacctta
    21241 ttttaatgct gaatatatat tataatatat ttaatgtagc ttaaatatag tttaaacata
    21301 aagaaaggaa acggtatatg ctaagaaaag ataaagttct attatctcta cataaattat
    21361 gtgaaaatat gactttaaaa gaccttcaat cagataaaac aggattcact acaatagaaa
    21421 ttgctaacca ttcatctctt gatagaagta ataccagtaa agaattaaat aacctataca
    21481 atgaaaaaaa agttctgaaa atatctggta agccaatttt atttctacat gctagtatta
    21541 tagaaaattt aattggccgt actttgaaca gtgatgaatt ttctataaat aattgtaatg
    21601 agctacttaa atcaagtaaa aatgactcag aagatatttt ttcaacatta cttggacatg
    21661 attctagtct taaactacct atagaaaaag ctaaatctgc tatactctat cctccaaaag
    21721 gtcttaatac gttactcata ggacctactg gtgttggaaa aagtacattt tccgaaacaa
    21781 tgtacaaata tgctgtagaa agtaaaatct tttcttctga aagcaaattc gtagtattta
    21841 actgtgctga atatgcagaa aatgctaact tattattaag taatttattt ggttatgtca
    21901 aaggttcttt tactggtgct aataaggata aattcggcat agttgcacag gctgatggag
    21961 gcatattatt cttagatgaa atacacagat taccaccaga aggtcaagag atgctttttc
    22021 ttttgatgga caaaggcata tacagacgat taggagaatc tgacattgtg cataaagcta
    22081 atgtctttat tgtgtgtgcc acaacagaag atatacattc ttctctgctt gggacttttt
    22141 taagaagaat ccctgttaca atcaacctac ctgctttaca ggataggtct ttaaaagaaa
    22201 gattatctct tattaaatac ttctttatta tagaatcaaa atacaccaat gcaactataa
    22261 aagttcacaa agatgtaatt aaatcattac tcctttatga ttgttttggt aatataggtc
    22321 agcttaaatc tgacatacaa cttatgagtg caagagcctt cttaaattac aaaactggac
    22381 ttaaggataa tatagaaata gatttatccc ttgttcctga ttacatctat attggtcttt
    22441 taaaattgaa tgaaaatcgt agtaatctaa ataaattatc agaacttgat tatatgcttt
    22501 tttatgaatt taatggtaat aacttagaag ttgaatatac aaaagaacta tctaaataca
    22561 atatagatac atcaaacaat ggatttaaat actctacaga gattccttat ctggaaaatg
    22621 atataaggag tatgtttata aattacatgg aaaagtactc tacaaaatta ctttcgatat
    22681 cttcagtaac tcattctgat tatacaagta atgagatttt taaagtaatc aatccaaaga
    22741 tttactatgc tgttgaacat tctttaaaag aggctgaaaa aatatcgaga aacaaataca
    22801 caaaacaaac ttatattgct ctttcaatgc atataagtgc acttatagaa aatataaatc
    22861 aagacacata taatcctaag tacaatttat ataatgataa aattttctta gataaagatt
    22921 taaatatagc taaaatagtt ttaagtataa ttgaaaatga cttacaaaca tctttaccta
    22981 aagatgaagt aaaatttata tccatgtttc taaatagcat gtatcttgaa ccaaacaatc
    23041 aatctgaaag tataggagtc ataatacttg ctcatggaaa ttcaacagca agcagtatgt
    23101 gtgatgtagc aaattcattg cttggtacaa accattgtaa tgcaattgac atgcctcttg
    23161 atgtaaaagt aagtaccata cttgataaaa ctatagagtt ggtaaaagaa tgtgacgaag
    23221 gtaagggtgt acttctttta acagatatgg gctctcttat agcttttgga gaacttattt
    23281 cagaaaaaac aaaaatcaaa actcgctcaa ttgaaatggt atctactcca atagttttag
    23341 aagcagtaag aaaaagtaca ctaccagaaa tgactttgga tgaattagct aattccttaa
    23401 gctctttaaa tccatatatt ggacgagcag caatcgattc aacagaaata aactatcctg
    23461 aaaagtcaag agagtatgta ataataacta cttgtataac aggatatggt tcagctatga
    23521 aagtagcaga ctttataaat tcttcactta aaaatattag taaatacaat attaatttag
    23581 ttcctcataa tatagaaagt tttaaaatgt ataagaataa atttagtaat actaaaattc
    23641 tggcagttgt tggaagtgta gattttaatg tagccaacac cccttttata cacatagagg
    23701 aactactaaa tggatatggc ctagatatgc taaacaaaat aatagaaggt acattcaatg
    23761 ccaatagtat tacaaatata aaagaaacaa atctatcata caacagttta ataataaact
    23821 ttatgaccca aaacttagag cttttggact ctgttaaatt attcaactat gtcaattctt
    23881 ccttatttac aataatagaa ttaatagatg aaaacttaaa gagcaaattt aagctaggat
    23941 atatactaca ttgctctttt atgatagaaa gatgttttaa aaatacaact tttccatata
    24001 ataacattac tgaattaatt agtaaaaatc atgaactata tagtttaata cgtgaagcct
    24061 ttaagccaac agaagcttat tttaatattt tgataccaga cgaagaaatt gcttatataa
    24121 tagacatgat taatgagtat ctaaataaat aaaatttata gcacttattt tgtgcatatt
    24181 aattaatata tgaaatggag gacgagattt tgaaaggaaa aataaaacag ttaacaatac
    24241 ttgcattaat ttttatattt ataactcctg tatttgcatt tgcagatact ccaccagtac
    24301 caaattcttc aagagcagca ctacttatag accaggaaac taaaagaata ttatttgaaa
    24361 aaaacattga tgaaaaaatg ccattagcga gtttaagtaa aatgatgacc tttttattgg
    24421 caatagaagc agttgataaa aatcaagtta aagagactga tatggtgaaa atagacaagt
    24481 ctacagcttc agtaggaggt tctacttgta aacttaaaga tggtgatgaa atatctctag
    24541 gagagcttat gcagggtcta atgctagtat ctggaaatga tgctgctata gccatagcta
    24601 aacatattgg taaaactgaa aagaattttg taaatatgat gaacaaaaaa gcagaagaaa
    24661 taggaatgat tgatacgtat tattttaatc caaatgggct tcctatatat actgacccag
    24721 aacataaaga acctcctatt gaaaatatgt caactgctca cgatatagtt acacttggaa
    24781 agtatatgta cgaccattat gaaaatcaag ttacaagaat aaccactatg caagtatata
    24841 atgatactaa aaaggatttt acacattata acacaaatcc tctgcttgtt tcagtacctg
    24901 gtgttgatgg aataaagaca ggatatacag ataatgctgg atattgcctt gcattttcta
    24961 tgatggtacc taaagatgct aaaaacgaaa gaaatcatag attgataggt gttgtacttg
    25021 gtgatggtaa caagaaaaat agaatttctt cttccgctac tttattgaaa tatggtaaag
    25081 ataatttcca ttctaaaaag atagcacata agggtgatat tatagagact ccttgtgttg
    25141 atggtattga tgattttaag ataacagtta aggttgataa agatttgtat ggtgttgttt
    25201 cagataacga aaatatcaat cctaaggtag tgtttaaaaa tatgaattat cctatccata
    25261 agggagatat tgttggtgtt gcaaagtatt ataatgactc tggtaaattt gttggaagtg
    25321 ttgatgtaaa aagtgaaagt aatataggat gtattccttt gaaggataag attaagatta
    25381 aggttgctaa gataaataaa aaacttgaga ttaaaaattc tgtttgtttt aaagcatagt
    25441 aaagcatatt attaaaaggg tatgttaaat ttataaagac tccactttgt ggagtctttt
    25501 tgttttttac aaaaaattaa aatagtggca acaattgagg atgtgaggga gagttgccac
    25561 tattttgtat agggtattat attatgtttt taagctagtt ttactatctt ctatgtttat
    25621 ataataactc ttgtgtgtga atttaattga taattaatat gaaaattatg tgaagttttg
    25681 atttatttgt aatatattct actactatta taatttttct actggatata ctaatcattt
    25741 gtttaacatt atctgagatt gatttgcaaa cttttatata actaaattag ttatataact
    25801 tatagattcc attaaatacc ctaataatca tatatgtaca tacaaagtca tgcttatgca
    25861 aaatctgcag tttcaactaa cttattatat aaaccatctt tatgtagaag ttcagcatca
    25921 tttccagatt ctattactct acctttatct aatacatata tattatcagc actcttaatt
    25981 gttgaaagtc tatgagctat tataataact atcttatctt gtatgtactc tttcaagttg
    26041 ttcaatatct ctttttgtga aatattatct agagctgaag ttgcttcgtc cagaataagt
    26101 acctttttat tacttactaa agctctagct attgatatac gttgtttttg acctcctgat
    26161 attttgacac ctttttctcc tactacttcg tccaatccct tatctagatt ttttataaaa
    26221 tcatctaaac aagtaacttc taatatctca tctaattctt tatcacttat tttattattt
    26281 aatactatat tattttttat tgtgtcatca aatacaaatt gatcttgagt tactatatca
    26341 atattatttc ttagtgattt taaattaata tcctttatat taacaccatc tattaatata
    26401 tcaccttcat ttatatccca aagtcggtat attagatttg ttattgttga tttgccacat
    26461 ccacttccac caacaattcc tgttacttcc cctttctcaa attgtaaatt tatatcattt
    26521 aaaacaaggt ttaaatcctc accatatcta aaagatacat ttttaaaatc tattttctta
    26581 atttcactgt tatctattct atatccatta ttatcaactt taatatctat cttttcatct
    26641 attactgaaa atactctatt gatagacact tttgattgtg ctattctagt attcgccctt
    26701 actatagcta tacatggtcc aattaacatt cctgtatatt gttgaaatgc tattaactcc
    26761 cccatcgtca tcttattatt tattatttta tatcccccaa ttccatatat taatattgtt
    26821 atcaatcctg ttagtatttg agatattgct atattactac tcataatgat gttcaaattc
    26881 aaatcttttt ttatcaaatc tctctctctc tttaaatact tattaaagaa attctttttt
    26941 gaatttaata taacaatatc cataatatta gatacgtact cttgaatcaa atttgataaa
    27001 ctgcctgaat cacttcttat ttcagaaatt ttcttagata taagttttgt cattttattt
    27061 tgagaaaatg ttaatatcaa ctgtaaaaat aaaactacta aaaataaatc aaactgcaaa
    27121 ttaattaaga aaattaacga tattatagct gtaaacacat ttacaataat atcaaatata
    27181 atctctgctc caaagttttc tatcataaat atatcacttt ccataatgga taaaatatta
    27241 cctgttttct tacttgaata atatctacca gaaagttttg atatatggtt aagcaacttt
    27301 acttttagat ttatagtaac tttatttttt attgaagtat ataaatatga taatataaca
    27361 tcaaatgtag ctgataatat ccttataaaa atatatatca atattaaatt tataagcaga
    27421 aaaatatttt tatttcctat tccatcatct attatacatc taattagttg aggtattaca
    27481 atatttacgc cagcaattat aaaaaccatt ataaatgata ctatcaactt agtcttattc
    27541 ttatatacta ttatatttag tattctaaat aaattattat taatagtttt tttcatctat
    27601 ttttccatct tttatagtta taattctatc agctgtcttt gcaatattat cattatgagt
    27661 tatcataact agtgtttgtt tgtactcttt aacacaccat tcaagcatat tcataacttc
    27721 atttgtagtc ttactatcta agttgccagt aggttcatct gcaaatatta ctgacggtct
    27781 attggctaaa gctctagcta ttgcaactct ttgttgttga cctcctgata gttcattcgg
    27841 atatttcttt atttgttttt caatccccaa cttattaatt aatctcataa catattcttt
    27901 atcttctttt ttcccatcaa gcaacacagg aagtactata ttatcatata catttataac
    27961 tggtattaaa ttgtaacttt gaaatatgaa gccaaattcc tgtcttctta tttcagacag
    28021 cttatcatca tttaatgtat ataaatctac atcatctaag tacacattcc ctaaagttgg
    28081 cttgtctaag cctgcaacac agtgaagtag tgtactttta ccagaaccac ttgcacctac
    28141 aatagctgta aatttatgtg gctctatatc taaatcaata ccatcaagag ctctaacttc
    28201 attctcatat tttccatata ttttttttaa atcttttacc ttaagtattt tcataacgta
    28261 tctccattcc catttaaaaa acatatattt ttaaattaaa ttttcgtaaa taagatctac
    28321 tgccatatct aaattctcta attgatataa gtatacttct ttgtagtcaa tactaatatt
    28381 aaactcttta tttaaaatag ttaaaaaatt atatgcatta taatttccaa tacatgtaaa
    28441 attgttaaat aaagaatatt cacttacaaa ttcaaagtct atttttattt tagaaaagtt
    28501 tctatttatc acatttatta ttttccttgc tatatccaat cttatcaaat tagatttata
    28561 acttggagtg ttcaagtttt ctatttttaa atcttcttta cttttgcttt tttgaataag
    28621 tatttcttcc aattcattaa acttaatata tggattgttt tttaatatat ttgcaacaat
    28681 gccagttgcc actgcacaag ccttactatt tttaccaaaa tgagttactt tatctttaaa
    28741 tttaataaaa ttatcttttg aatttgcata catttgaatc ttattttctg gttcgtatat
    28801 atattgatta tttttatata tattttcatt acctactaca ccaataacac tttcaaatat
    28861 agtaggaata ctttctttat cttcatattt attatgtttt gaacaaataa taaatctacc
    28921 ttgactattt aaaatattac aaaccttttc taattctttt tcatacatac tattaataga
    28981 tgcagctgaa atgtttatta tctgtatatc cgacatagtc aacttgctca gtgtactaag
    29041 aagatttaaa gaacttgttt ttccagaatc attaaatata ttaataggat aaatttgggt
    29101 atttttactt atatttaata tggttttcga tacaaaagtt ccatgtccat ttatgtcatt
    29161 tatttccttc ccccccaaat ctttcaattg aaaatttgaa tcataaacat aatttccatt
    29221 aatatcatca tcatttacat ctaatcctgt gtctaagact gctattctta ctttagtcaa
    29281 ttaataatca cctctaaaat aaattacctt gaatataaaa ctaatcctta ccataatata
    29341 ggttccaaga tgtttttcat ttttttacaa tttgcatcaa acattttttt gttatttttt
    29401 aaagtatata ttttcgatgg acaagttgta caaaataaat ttactttaca ttctttacaa
    29461 ctctcaatat ttactggttt taatttatca aatatattaa atatatccat ttctctatta
    29521 agaatcctct ctaatatgtt ttcattaaat tctaatatat tgaatatctt taaatcctta
    29581 tactctaagt ttggacacgg atatacatct cctttatgat ctatacttat ctgattaaca
    29641 cctgctttac acatatttga acataaacta ctttcatcca cataatcatc tttgcaaatg
    29701 taatcaatat caagtttatc attaagatat ttgctatagt tctcattacc tctaccaata
    29761 ttcataaaat ttcttattat aggcgttgta cctaaatctt catttaattt attgaatttc
    29821 tctatgtctt tctcattatt ctttcctacc accatagaca aacctacgtt ctcgaatttt
    29881 tttagtttta taagtctaat cacatttaat actttacaga atactccttt acctcttact
    29941 tcactacaac ttaattcatc ataaccatct aaactaattt ctattgcata taaaccatta
    30001 attatatcat caatctgttt ttctttaatt aaagttgcat ttgtagacag aattatttta
    30061 cctttaaatt tttcttttat ataatccaac agctctaaga aatcatttct catcaatggc
    30121 tctccacctg taatggttaa tatccttgga tttaatttaa ctatattatc tattactttt
    30181 cttacttgag atgtatctaa ataatcgtta ttatctattt tagaatctac acagcaataa
    30241 tcacaactta gattacatct attagttgga gaaaatgaaa ttttgtttat tctctctagt
    30301 ctaaattcac ttagaataga atcacttaga agtcctatat gctctaactt atctataact
    30361 cgttcataat attttttatc ttcattttct tggaatacat catacaaatt accaagttgt
    30421 aatgcattat ctactgaata ctttagaact tcataacatt ctattggaat tttaacccat
    30481 cttccattta ctttattact aagaataact tgattattat ttattatttt ttcacatata
    30541 caatctaaaa aatatttgtt tgagctattc atttatttta tttccttcct atatatttaa
    30601 attagaaatt tcattaattt tcttttatac cacttactat actaagttta tttatcattt
    30661 tattagaaaa ataaactgca atgacacaaa caaaaacaga tatagatcca aatattagaa
    30721 tttcatatat tggaatttca aatttaatag agttatttat accatatacc tgttgattaa
    30781 gtttgttagt ttcagaaact attttataat aattgaaact agatataatt ccagctacaa
    30841 tcatacttaa taccccatat atcatatttt cttttattaa cattcttttt acattttttt
    30901 tggacatccc tattgctcta aatgtacata tctcactcaa cctcacaatt atatttgatc
    30961 taactataaa aataatgttt atagatgata ttaataaaac tccaaataca caaatcattg
    31021 ctaatttata tgaggattta ttataatggt ctccaaaatg attacgtaca tatcttccct
    31081 ctatatctga aaatccactt ttttctatta tattatttat ttcttttatt acttttttat
    31141 ctgtttcttt ttttatcttc acaaaaactt tattataatc acttatccct gttaattctt
    31201 ttaaatcctt ttcattaaat ataatttgaa ttccacctga ttgagattct ccatcttgct
    31261 ctgatatata gctatttttc attattccag cgattcttac ttttgtattt atatatttat
    31321 actctccatc tttatataca ggaattttta tatctattaa gtctcctatt ttaggtgatt
    31381 ttataatctg agtattattt gacaatttaa ttctagaata aaaattattt accaataaaa
    31441 catttttata atccctgtct gtataactat aaacattttc gcctttatct atatatttat
    31501 ctatattatt taatagctct ttattgtaac ctcgaattaa aactgtagac tcattattat
    31561 tatttatttc agttcttttt agctcatcta aataatcttc ttctaagtca gcatttctta
    31621 gatatccttt taaataaata ttgggttcaa catctgttac atcttcaata gcttttattt
    31681 catttaataa agaattatca tatttagaaa atgtataatt tgcatttatt ggtgaatatc
    31741 ccaaataaat atcaaaattg ccatatgatt tacttgttat attagctgaa tctgactctt
    31801 ttccatatat gaattttgta gaagttagtc tgcctataaa taaaacccca catagtgata
    31861 tcgtaattat tattattatt gatgtaacct tattcctaaa tatattttta aaagctatct
    31921 catttataat tccaacaact tttttatgtg attttaacaa caaaagccta ggaatataat
    31981 ttaacttatc tgtctttttt atagcatcaa ttatagataa atttccagct ttatatattg
    32041 gtactacaga ggctaatata actgctataa ttgacatgat gatagattca attatagagt
    32101 ttgtatttat agttaattct gcacttttat catatactat catgagacct aagtaagaaa
    32161 aaactatacc tccaagtaaa ccaattacta tacctacaga taaaactaca atactctgaa
    32221 taaaaaatac ctgtctaata tttctcttgc tagctccaac agctctaagt aaaccaattt
    32281 gcttggtcat atctagtatt attatgttaa acatattgta tattactaat gttgcacata
    32341 ataaagggag taagctttgc tttattgaat aagttgtttt ttttgaaata tttttcatat
    32401 acatagacat acttacttct gtatttggat aaaattgatt acttgttaac ttatattttc
    32461 tccctagatt cactactttt tgagaatcta aatcttttgt attcaatttt aatgtaccca
    32521 tatgagttat taattcatat ggaacaatat ctttgccatt gtttgaatat gtaaaagctc
    32581 ttaccttata tttatttaaa ttttcatagt attcatcaga tttacttaaa ataccaacta
    32641 ttttaaattg cttatcttta ctataaatag aattttcatt atttttattt atatgctcat
    32701 taacaatctg aaaatcaata gtttgattta atttgacatc taattccatt ttgtctaaaa
    32761 ctttttgttc tattactaat tcattagaag ttctaggctc tctaccttta actaacttat
    32821 atctagtaga atctaaaaaa tctttattgt aagagcataa atttagagat gctccattat
    32881 ctttacttat aatttctcca aatttaagta catcattgaa ttcttttata tcttcttcac
    32941 cattaagttc ttttactata tcactattta tatcttcata aaaagcatga taatctcccg
    33001 agtttttgta tgcatgattt atatctgatt gtccttgaga ttcattaata acacttaatg
    33061 aaaaaactag cataacagca atccctatac taagtattaa tgataaagtt tttccttttt
    33121 gtctctttaa atatgacacc gcaagtttaa tatttatcat aatcagaatc ctccttattc
    33181 atatttacaa gaaaaactct ataacctaaa atagtaggtt atagagcact attcattaaa
    33241 tactaactaa ctcgttgtgc tagttaaatt taattattaa aaatactaga gttgagaaaa
    33301 tcacatttca tttgtaaaaa actacatcta gtactttcaa catatttaat agactgatta
    33361 gtatttttta ataagctact gttccttttg gagttgatcc tgcacaatcc accatacagt
    33421 ctatttgtga aattattcca tgcttatcat aataccattt acatggatct gcttgcacct
    33481 caacatcttg aagttctatt ttttcttcga ttttttttat atgtatcata atacatatcc
    33541 ccctttactt ttgttttata tttattataa ctagttaaac tagattataa actacatagt
    33601 ttttttgttg tttccacata aactctttct aatccacaat aaattggtac tgcactttgg
    33661 aatttacctg ttaccttttt attgataagt ttacatctgg ttgacataca ataatcaagt
    33721 ctagtacatc catcacactc ttcattctta gatatataaa tttttataag gttcttaatt
    33781 tttttatcat ctaatccatc atatatattt cctagtaaaa tatctttatc tcctacacat
    33841 actgtacatg gatatacatc tcctctagta tctattgcat aactatttat ccctccaaaa
    33901 caatctcctt ttttcttatt tataaattgt ttatctaaaa tagatatatc tatatcagga
    33961 taatcctttt taatattcat aattttacta atttgttcat ttaatatttt aatatgagaa
    34021 ttatcccaat ttttatccat aaaatctgaa attggtgcga tatttttaaa cccttgtttt
    34081 gctatatcat atacactctc ataaagatac ttaacagtat ttgagttata tgtcattctt
    34141 gccctaacat atataccttt atctataagt ctttttccat tttccattac tttttcataa
    34201 gtacctactc cgttagcatc tattctattt atatcatgta tattcttttt tccatcaata
    34261 cttatcgata tgttttttac attctttgct ataaactcta ttatttcatc atttataata
    34321 gttccatttg tagttatatc aaataatatc tttttgtcac tcaatctatc tactaaatcc
    34381 ctatgtatat atttaatcaa atcaaaattt ataaatggtt ctcctccatg taaaacaatt
    34441 gttgtataat cactattact ttctaattct ttattacaaa taaaatctat tgttttatca
    34501 gcagtttcat aactcatatt ttcttttttt ttatcattac cttcataaca atatgtacat
    34561 ctcaaattgc agtcttcagt tacaaatata gtaaatttca attaactttt tcatctcccc
    34621 atacagctgt atttaaattg tgtttcatca tactacacca tctatccaat tccaattcat
    34681 tatccttaac tgaatctaat atcgctgggc aatgccaaca aaaaatattt acatcacaat
    34741 ctttacattt ttcaaagtta tatggataaa tactttctaa tttaatcaaa gactttagat
    34801 ctcttgtttt attttctttt ataactttta caaattcatt gtcaaatata tttcccattt
    34861 tatagtcatc ctttatcagt gaaggacaag gatatacatc tccatcataa tttataaata
    34921 tttgagagtt tcctgcatta catgcacaac atcccatttt tttatttttt ttatctttac
    34981 acatttttaa ttcattatct aaaatatacg gaattgtaat tggaagaact gtatcttcaa
    35041 ttaaatagct ttctttattt tctaggcctc ttcctgttgg aataaaatat ctttcaacaa
    35101 attctgttcc aagcttttta tttaaatctt taaattgttt atttaaatga gaattttttt
    35161 gtccaaatac catagacagc tttatattac tgaatccatt gtcttttagc agattaacag
    35221 agcttattac tttattaaat acaccttttc ctcgtgtttc actacaagta ttttcatcta
    35281 ctccatcaat actaatatct attttttcaa aattagttat aataaaatca acattatgtt
    35341 catctattaa agttccattt gtggataata ttaatttggt atcaggtaat ttttgtttta
    35401 tatatttaat tatttcaaaa aaatcttctc ttatcaatgg ttctccacca gttataatta
    35461 tatgaggagt atttaaaact gatatta
//
LOCUS       DAGSWI010000028        34806 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.028, whole genome shotgun sequence.
ACCESSION   DAGSWI010000028 DAGSWI010000000
VERSION     DAGSWI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.028"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            263..739
                     /gene="purE"
                     /locus_tag="MJF80_001240"
     CDS             263..739
                     /gene="purE"
                     /locus_tag="MJF80_001240"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBZ0279912.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            745..1443
                     /locus_tag="MJF80_001241"
     CDS             745..1443
                     /locus_tag="MJF80_001241"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBZ0279913.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            1455..2822
                     /gene="purF"
                     /locus_tag="MJF80_001242"
     CDS             1455..2822
                     /gene="purF"
                     /locus_tag="MJF80_001242"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBZ0279914.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            2816..3892
                     /locus_tag="MJF80_001243"
     CDS             2816..3892
                     /locus_tag="MJF80_001243"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBZ0279915.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            3886..4479
                     /locus_tag="MJF80_001244"
     CDS             3886..4479
                     /locus_tag="MJF80_001244"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBZ0279916.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKIKLQGRRVFINE"
     gene            4472..6004
                     /gene="purH"
                     /locus_tag="MJF80_001245"
     CDS             4472..6004
                     /gene="purH"
                     /locus_tag="MJF80_001245"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBZ0279917.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            6015..7265
                     /gene="purD"
                     /locus_tag="MJF80_001246"
     CDS             6015..7265
                     /gene="purD"
                     /locus_tag="MJF80_001246"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBZ0279918.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            7290..11096
                     /locus_tag="MJF80_001247"
     CDS             7290..11096
                     /locus_tag="MJF80_001247"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBZ0279919.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            11302..12174
                     /gene="rfbD"
                     /locus_tag="MJF80_001248"
     CDS             11302..12174
                     /gene="rfbD"
                     /locus_tag="MJF80_001248"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBZ0279920.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            12283..13152
                     /gene="rfbA"
                     /locus_tag="MJF80_001249"
     CDS             12283..13152
                     /gene="rfbA"
                     /locus_tag="MJF80_001249"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBZ0279921.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGINITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            13201..13758
                     /gene="rfbC"
                     /locus_tag="MJF80_001250"
     CDS             13201..13758
                     /gene="rfbC"
                     /locus_tag="MJF80_001250"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBZ0279922.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            13796..14779
                     /gene="rfbB"
                     /locus_tag="MJF80_001251"
     CDS             13796..14779
                     /gene="rfbB"
                     /locus_tag="MJF80_001251"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBZ0279923.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVNSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKVILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            14809..15477
                     /locus_tag="MJF80_001252"
     CDS             14809..15477
                     /locus_tag="MJF80_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBZ0279924.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            15486..15914
                     /locus_tag="MJF80_001253"
     CDS             15486..15914
                     /locus_tag="MJF80_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBZ0279925.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            15938..16252
                     /locus_tag="MJF80_001254"
     CDS             15938..16252
                     /locus_tag="MJF80_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBZ0279926.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            16404..16679
                     /gene="flgM"
                     /locus_tag="MJF80_001255"
     CDS             16404..16679
                     /gene="flgM"
                     /locus_tag="MJF80_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBZ0279927.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            16684..17100
                     /locus_tag="MJF80_001256"
     CDS             16684..17100
                     /locus_tag="MJF80_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBZ0279928.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKKTTTYNSYGNIRK"
     gene            17118..18428
                     /gene="flgK"
                     /locus_tag="MJF80_001257"
     CDS             17118..18428
                     /gene="flgK"
                     /locus_tag="MJF80_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBZ0279929.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            18447..19379
                     /gene="flgL"
                     /locus_tag="MJF80_001258"
     CDS             18447..19379
                     /gene="flgL"
                     /locus_tag="MJF80_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBZ0279930.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            19395..19787
                     /locus_tag="MJF80_001259"
     CDS             19395..19787
                     /locus_tag="MJF80_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBZ0279931.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            19781..19993
                     /gene="csrA"
                     /locus_tag="MJF80_001260"
     CDS             19781..19993
                     /gene="csrA"
                     /locus_tag="MJF80_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBZ0279932.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            20015..20410
                     /gene="fliS"
                     /locus_tag="MJF80_001261"
     CDS             20015..20410
                     /gene="fliS"
                     /locus_tag="MJF80_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBZ0279933.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            20410..20766
                     /gene="fliS"
                     /locus_tag="MJF80_001262"
     CDS             20410..20766
                     /gene="fliS"
                     /locus_tag="MJF80_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBZ0279934.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            20787..22310
                     /gene="fliD"
                     /locus_tag="MJF80_001263"
     CDS             20787..22310
                     /gene="fliD"
                     /locus_tag="MJF80_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBZ0279935.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            22313..22648
                     /gene="fliT"
                     /locus_tag="MJF80_001264"
     CDS             22313..22648
                     /gene="fliT"
                     /locus_tag="MJF80_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBZ0279936.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            22750..23622
                     /locus_tag="MJF80_001265"
     CDS             22750..23622
                     /locus_tag="MJF80_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBZ0279937.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            23715..25856
                     /locus_tag="MJF80_001266"
     CDS             23715..25856
                     /locus_tag="MJF80_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBZ0279938.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            25880..27757
                     /locus_tag="MJF80_001267"
     CDS             25880..27757
                     /locus_tag="MJF80_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBZ0279939.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            27750..29864
                     /locus_tag="MJF80_001268"
     CDS             27750..29864
                     /locus_tag="MJF80_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBZ0279940.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            29873..30868
                     /locus_tag="MJF80_001269"
     CDS             29873..30868
                     /locus_tag="MJF80_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBZ0279941.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            30960..32192
                     /locus_tag="MJF80_001270"
     CDS             30960..32192
                     /locus_tag="MJF80_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBZ0279942.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            32212..33156
                     /locus_tag="MJF80_001271"
     CDS             32212..33156
                     /locus_tag="MJF80_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBZ0279943.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            33250..33657
                     /locus_tag="MJF80_001272"
     CDS             33250..33657
                     /locus_tag="MJF80_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HBZ0279944.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNM"
     gene            33744..>34806
                     /locus_tag="MJF80_001273"
     CDS             33744..>34806
                     /locus_tag="MJF80_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBZ0279945.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISEGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKNGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVS"
BASE COUNT        14317 a         3458 c         6421 g        10610 t
ORIGIN      
        1 aagatttaaa aaagagtatt gggcttaaaa tttaattaga aaaccaattt tgagataatc
       61 tgttttttat tttaacaaaa aaacataaat aaagttcgat acgtagcgaa tgaaaaaatt
      121 acaagaaata ttttttttaa aaaatttcaa aaaagagttg ataatatatg tatattaata
      181 taaaatttaa ggtgtataat tcgtataata atacgaatga tttgtagtgt aaaataactt
      241 ataaacggag aggggaagtc taatgaaagt agcagtagtt atgggttcaa aatcagatta
      301 tccaaaatta gaagaaggga taaaactgct tgaaaaatat ggaatagaag ttgtagcaag
      361 agcattatca gctcatagaa caccagaaca actttcaata ttcctaaaag aaatagaaga
      421 tgatactgat gtaataatag cagcagcagg aaaagcagca cacttaccag gtgtaatagc
      481 ttcacaaaca ttaattcctg taataggatt acctattaaa tcatcaacta tggatggact
      541 agactcactt ttatcaatag ttcaaatgcc aaaaggaatc ccagttgcaa cagttacaat
      601 agacttaggt ttaaacgcag ctttattggc attacaaata atgactttaa aatacccaaa
      661 attaaaagag gatttgaaat catatagaga agagatggca caaaaggtgt tagaagacga
      721 caagaattta agggggtagc agaaatgtta ttatatgaag gaaaagcaaa acaagtatat
      781 tctacagata atgaaaatga atatgttgtt tattataaag atgatgcaac agcatttaat
      841 ggagaaaaaa aagcagaaat atcttcaaaa ggtatactaa ataataagat atcaacaata
      901 atctttgaaa tgttaaaaga aaataatata aatactcact ttataaaaag tttatcagat
      961 agagaaatgt tagttaaaaa agtagagatt ttaccattag aagtaatagt aagaaatatt
     1021 gctgctggtt ctatctgtaa aagagttggt cttgaagaag gtgtagtttt cgatgagcca
     1081 atttttgaaa taagctacaa aaatgatgct tatggggacc caatgctaaa tgatgattat
     1141 gcagtggcaa tgaagttagc aactagagaa gagttaaaat tcttaagaga agaaacttta
     1201 aaaataaatg agttactaaa agcatttttc ttaaaattaa acctaaagtt agttgacttt
     1261 aaaatagaat ttggtaagga ttctgaagga aatataatat tggcagatga agtatcacca
     1321 gacacttgta gactttggga tgtaaataca aatgaaaagt tagataagga cagatttaga
     1381 aaagaccttg gagaccttgt agaaggttat acagaagtac tttcaagaat gaataataaa
     1441 taggaggcaa atgtatgtgt ggagttttag ggatttactc taataaagat gtaactaaag
     1501 aactatatta ttctttatat tctatgcaac acagggggca agaaagctgt ggtttagctc
     1561 ttttagatga tggagaaatt aagtacaaaa aagatatggg attagttgga gatgttttta
     1621 aggaaaatga attatctaag ttaaaaggaa atatagggat tggacatgtt agatattcaa
     1681 cagctggagg aagtcatgtt tctaattgtc aacctttagt tggaagttgt agaaaaagac
     1741 aattagccat agctcataat ggaaatctag ttaatgcaaa ttatttaaaa gatatgctag
     1801 aagaagatgg atatatgttc caaactaatt cagatacaga agtgatttta tatatacttg
     1861 ctagatatta caaaggggat atagtagaaa gtttaaaagt gacaatggat tatataaaag
     1921 gtgcatatgc tcttgtaata atgagtcaag aagaattggt tgcagttaga gacccacatg
     1981 gattcagacc tcttgtactt ggtaaaaagg gtgatgaata tatatttgca tcagaaaatt
     2041 gtgcaataga tatacttggc ggagaagtta taagagatgt agagccagga gagataatag
     2101 tagtcaaaga tggagaacta aaatcatatt tttattcaga aaattataaa ccagttaaga
     2161 aaagttgtat atttgaacac atatactttg caagaaatga tgcaactata gacaatgtaa
     2221 atgcatatga atttagaatt aaatgtggtg agagattagc tcagaatgaa actgtaaagg
     2281 cagatatggt agttccagtt ccagattcag gttggccagg agctattggt tatgcaaatg
     2341 ctagtggatt aaaaataagt gaaggcttag ttaaaaatag atatgtagga agaacattta
     2401 taaaacctac acaagaagaa agagaaatag cagtaaaaat aaaattaaat cctctttcaa
     2461 caataataaa aggaaaatct ataattttag tagacgactc aattgtaaga ggaactacat
     2521 ctaaacaatt agtaaaatct ttaagagaag caggagcaaa agaaattcat cttagaataa
     2581 cttctcctcc agtagcttac tcttgttact atggaataga tactccaaat cgctctaaat
     2641 taattgcatc aagtaacaac gtagaagaaa tgagagaata tattggatgt gatagtttaa
     2701 aattcctaga tatagaagga atgttagatg caacagaaca taagtctact ttctgtaaag
     2761 cttgttttga tggagaatac ccagttaaga aaattgataa ggaggaacta ctttcatgtt
     2821 aacatataaa gaatcaggtg tagatataga tgaaggaaat agagcagtag accttataaa
     2881 aggtaaaata aaaggaactt atgatggaaa tgtagttgga gatttaggta acttcagtgg
     2941 attatacagt ttaaaagatt ttgtaggtat gaaagaacca gttttgcttg catctacaga
     3001 tggtgttggg actaagttaa aaatagctca gatgatggac aagcatgata ctgttggaat
     3061 agatttagtt gcaatgtgcg tgaatgattt aatttgtcaa ggagcaaaac cattattctt
     3121 ccttgactat atagctcttg ggaaattagt gcctgagcat atagaaaaaa tagttggtgg
     3181 aattgcagat ggttgcaaaa tgtctggttg tgcactaatt ggtggagaaa ctgctgaaat
     3241 gcctggaatg tatggagaag atgactatga tttagcagga ttttctgtgg gaatagcaga
     3301 caaagaaaag attgtttctg gaaacaatgt taaatcagga gatgtattgg ttggtatatc
     3361 ttcaagtgga gtacacagca atggattttc ttttataaga aagatatttt tagaaactta
     3421 caattataaa atggaacaat atgttgaaga actaggaatg acagttggag aagcactttt
     3481 aacaccaact aaaatatatg ttaaattagc tttagatgta ttagctaaac atgacataaa
     3541 agctatagct cacataactg gtggagggct tatagaaaat ataacaagag ttatacctaa
     3601 agggttagga ctagatatca ataaaaaatc ttgggagaag cctcctatat ttaaaatgat
     3661 agaaggtttt aatgctgttg atgagagaga acttcataag agttttaaca tgggtattgg
     3721 gctagtttta atagtagata aagaaaatgc tgatgatgtt gtaaacttta taaataatag
     3781 agaaaatgac aatgctgatt atgtagataa aaaatatagt gaattattag aagataaagc
     3841 ttatataatt ggtgaagtag tagatagtca tgaaggtgta gaattatgct aaatatagga
     3901 gttttaatat ctggtggggg aacaaattta caagcagtta tagatggtac tgagtctgga
     3961 gaaataaaag gtcaggttaa agtagttatc tcaagtaagc aaggtgctta tgggctagag
     4021 agagctaaaa atcataacat aaaagcaatt tgtgaaactg atgaagacaa gataatagaa
     4081 attcttaagg aaaataaaat agatttagta gttttggctg gttatttaaa aataatcagc
     4141 ccaaagctgg ttaatgagtt tagaaacaaa atgataaaca tacatccatc attaattcct
     4201 tcattttgtg gagctggatt ctatggagaa aaagttcatc aaggtgttat agattacgga
     4261 gctaaagtaa ctggtgctac agttcatttt gttgatgaag gtgctgatac tggaccaatc
     4321 ataatgcaag atgtagttaa ggtaaatcaa gatgatgatg caaagacact tgcaaaaaga
     4381 gtgttagaag ttgaacatag gattttaaaa gaaagtataa gtcttttttg tgaaaataaa
     4441 ataaaacttc aaggtaggag agtgtttata aatgagtaag agagctttaa taagtgtaac
     4501 tgacaagact ggtgtagtgg aatttgctaa agagttaaat aaattagatt atgaaattat
     4561 atctacagga aatacattta aaacattaaa agaaaatggt gttaatgtaa tgcaagttga
     4621 agatgttaca aattttccag aaatattaga tggaagagta aaaacattaa atccatatat
     4681 acatggtggt atactttata aaagagataa agaatctcat gtagaaactg taaatgaaca
     4741 caaaatacat tcaatagatt tagtagctgt aaatctatat gattttgaag gaactttaaa
     4801 agctggaaaa agtcatgatg aaataattga aaatatagat attggtggac catctatgat
     4861 acgttctgct gccaaaaact ataaggatgt aatagtagta gtagatataa aagattatga
     4921 ttctataata gaaaaattaa aaacagatac aatgacatta gaagacagaa aaaaattatc
     4981 atataaagct ttctcaacta caggaagata tgatgcgtta atatctagtt attttgctgg
     5041 agaagttgga gatacgtatc ctgacatatt aaatttaact ttccaaaaag aacaaacttt
     5101 aagatatgga gaaaatccac atcaaaatgg tttcttatat tcacagtcaa atgcaaaaaa
     5161 tccaatctta aattatgagc aattaggcgg taaagaactt tcttttaaca acttaaatga
     5221 tttacatgga tgtttagaag taatgagaga atttaaagat agtgaagaag tagtttcagt
     5281 agctataaaa catgctaatt catgtggggt agggttagga aaagatgctt ttgaagcata
     5341 tacaaaatgt tatgaagcgg ataaggtttc aatatttgga ggaatagtag gaataacttc
     5401 tactatagat aaagctacag cagagaaatt aaatgaaata ttcttagaaa tagtagtagc
     5461 atatgatttt gaaccagaag ctttagagat acttaaacaa aagaaaaatc taagaatatt
     5521 aaaattagct aaaatagaaa atagtttaca accatatgaa atgaaatatt tagatggaaa
     5581 attattgata caagatagaa acaatatatt agcagaaaaa agtgaaaatg taacaaagga
     5641 aaaaccaaca gatgcacaac ttaaggacat ggaatttggt atgagagtag taaaaaatat
     5701 gaaatcaaat gcaattgcaa tagtaaaaaa tggacaaact ctagctttag gttgtggaca
     5761 aacatcaaga atttgggcat taaaaaatgc tcttgaaaat aacaaagata aagattttac
     5821 aggagctgtt ttagcgtcag acgcattttt cccatttgat gattgtgtaa cattagctca
     5881 tgaatatgga atatcagcag tagttcaacc aggtggctct ataaaagata aagattcaat
     5941 agaagcttgt gacaagtatg atatggttat ggtatttaca ggaataagac acttcaaaca
     6001 ctaaggagga tattatgaag atattggttg taggcggtgg cggaagagag catgctatat
     6061 gctggaaact aagcaaagaa aaaaatgtag aaaaaattta ttgtgcacca ggtaatgcag
     6121 gaattgcaaa cgtagcagaa tgtgtaaata taggtgatac taatatagaa gaacttttaa
     6181 aatttgccaa agaaaatgaa ataggtctga caatagtagg accagaagta ccactagtta
     6241 tgggaatagt agatgagttt gaaaaagaag gattaagagt atttggacct aataaaaaat
     6301 gtgctcaact tgaaggaagt aaagcttttt caaaagaatt tatgataaaa cataacatac
     6361 ctactgcaaa atataaagaa tatacaaact tagaagaagc tatatcagaa atagatagtt
     6421 ttggatatcc agtagtaata aaagcggacg gattagctgc tggaaaaggt gttgtaatac
     6481 ctgaaaatag agaagatgct atagcaactt taaaagaaat gatgtcagat aagaagtttg
     6541 gagctgctgg agataaaata gtaattgaag agtttttaaa aggaatagaa acttcaattc
     6601 tagcatttgt agataatgat acaatagttc ctatggcaag tgcaaaagac cataaaaaag
     6661 taaacaacta tgagcaaggt ccaaacactg ggggaatggg aactttttca cctagtgaaa
     6721 tatatacaga agaattagca aataaagtaa aagagacagt attagaaaaa actttagaag
     6781 gcttcaaaaa agatggactt aattttaaag gtattttatt tgttggtctt atgataactg
     6841 aagatggaga aaaagtactt gaatataatg taagatttgg agaccctgag acacaatctg
     6901 ttttatttag attagaaaca gatttacatg agataatgga agctatacta gataataagt
     6961 taaaagatat agaaataaac tatagtgatg atgaagctgt gtgtgttatg cttacatcag
     7021 gagggtatcc agacagttat gaaaaaggga aaattataac tggtcttgaa aacttggatg
     7081 atgatatagt agtattccat agtggcacta agatgtttga tggtaatttg gttaccaatg
     7141 gaggaagagt aattggtata actgcaaagt caactacagt taaggatgca gcagaaaaag
     7201 tttatgaaaa cataaagaag ataaactttg aaggaatgca ctatagaaca gacataggaa
     7261 gataattttt ctaaaatgga ggataaggta tgttgaatac tgaaaataaa gactcaatgg
     7321 ttagaagagt attagttgaa aaaagagaag gttttgacct tgaggctaaa gctttaaaaa
     7381 aagacttagt agaaagttta cacatagata atatagaaaa tttaagaata ttaaatagat
     7441 atgatgtaga aggaatatca gaagaagttt atgaaaatgc agcaaagaca atattctctg
     7501 aaccaaattt agatgtagta tattatgaag aaataccaaa attaaatgat gaaagagtat
     7561 ttgcaataga atttcttcct ggtcaatacg accaaagagg tgattgggca gctcaatgtg
     7621 tacaaattgt aaatcaaggg ataagacctg ctattaatac agcaaaagtt tatatattat
     7681 caggcaaaat aactgatgaa gagttttcta aaataaaaga ttactgtata aatccagtag
     7741 atagtagaga agcatcttta gaaaaaccag aaacgttaaa aatggaaact gaaataccaa
     7801 caacagttga agtattagat ggatttatag atttagatga aaatggatta aaaacatttg
     7861 taagtgaaaa aggattagca atgactttag gagacttaca acacgttcaa aaatacttta
     7921 aagatacaga aaagagaaat ccaactataa cagaaataaa agtattagat acttattggt
     7981 ctgatcattg tagacatact acttttatga ctgaaataga aaatgtaaag atagaagatg
     8041 gtaagtttaa tgatatagta aaagaagcgt atcaaatgta tcttaactca agagacaatg
     8101 tatatgtgaa cagacataaa gatatatgtt taatggatat agccactgtt gcagtaaaag
     8161 aattaaagaa aaatgggaaa ttaaatgacc tagatgaaag tgaagaaata aatgcatgta
     8221 gtataaacgt agatgttgaa gttgatggta agatggaaaa atatctagta atgtttaaaa
     8281 atgaaactca caaccatcca actgaaatag aaccatttgg tggagcagct acttgtttag
     8341 gaggagctat aagagaccca ttatcaggaa gaagttatgt ttatcaagca atgcgtgtta
     8401 caggaagtgc tgacccaaga acgactttag aagatacttt acctggaaaa cttatgcaaa
     8461 gaaaaataac tacagaagct gctcatggat atagttcata tggtaaccaa ataggtctta
     8521 caactgggca agttgcagaa gtatatgatg aaaactttgt tgcaaagaga atggaaatag
     8581 gtgcagttat agcagcagca ccaaaagaga atgtagtaag agaaagacct gaagcaggag
     8641 atgtaatcgt tcttcttgga ggaaaaacag gaagagatgg ctgtggagga gcaactggtt
     8701 catctaaaga acatagtgaa gagtcaatct taacttgtag tgcagaagtt caaaaaggag
     8761 atgctcctaa cgaaagaaaa atacaaagat tctttagaaa taaagaagtt gctcaaatga
     8821 taaaaagatg taatgatttt ggagcaggtg gagtttgtgt tgctatagga gagattgctg
     8881 atagcttaga tataaactta gacttagttc ctaaaaaata tgatggactt gatggaactg
     8941 agcttgcaat ttcagagtca caagagcgta tggcagtagc cataaaaaaa gaaaataaag
     9001 ataaatttat acaattagca gtagaagaaa acttagaagc tacacatgta gctacagtaa
     9061 cagatacagg atatttaaga atgttctgga atggaaaagc aattgtagat ataaatagag
     9121 agtttttaga tacaaacgga gtaaaacaaa ctactgatgt tcatgttact aaagtagatg
     9181 aagaaaatac attctttagc tctaatgaaa tagtaaaaga tgttaagtgt gcaagtatga
     9241 aagacaagtt tacaaaagta ctatctgacc ttaatgtttg ttctcaaaaa ggattagtag
     9301 agatgtttga taatacaatt ggtggtaata cagtccttat gccatttgga ggaaagtatc
     9361 aagcgactcc aacacaaggt atggttgcta agataccagt acttggagga gaaactaata
     9421 cttctacaat aatgacttat ggatacaatc caaaggtagg aaaatggagc ccattccatg
     9481 gtgccttata tgcagtagta gaatctgtgt gtaaattggt tgctattgga ggaaattata
     9541 gtacaacaag acttacattc caagagtatt ttgaaaaact tggtaacaat ccagaaaaat
     9601 ggggtaaacc attctcagca cttttaggag cattttatgc acaaagtaaa tttgagatac
     9661 ctgctatagg tggaaaagac agtatgtctg gtacatttaa agatattgaa gttccaccaa
     9721 cattagtttc atttgctgtt gacacagtag atgcgaagaa agtagtatct ccagaattta
     9781 aaaaagcaga ctcaaaagta gtaatgttat gtgtaaacaa ggcagaaaat gatgttgtag
     9841 attttgagga attaaagaga aacttagata aagtaagaga acttatacat ggaaataaag
     9901 ttttatcaac atatgcatta ggatttgcag gtgtaggtga agcaataagt aaaatggctt
     9961 ttggtaataa aataggattt aagtttagtg aagaggctga aaaagcattt acagatgata
    10021 aattatttga ggctagttat ggaaatatag tacttgaatt agcaaatgat gatttaagta
    10081 tgttagaagg atacaattat gttgtgcttg gaagtacagt taaagaagct agtatattta
    10141 tcaaaggtga agaacttgca ttagatgaat tatataaagc acattgtagt actttagagc
    10201 ctatattccc aactaaaaca gaagaagtaa aatcaaagat agaaactata agttatatat
    10261 cacaaggaga agctaaaaaa tcatcattaa gtatagctac tccaagagta tttataccag
    10321 cattccctgg aacaaactgt gaatatgact ctgcaagagc atttgaaaga gcaggagcta
    10381 atgcaagtat aagagtattt aagaatttaa cttataaaga catagaagat tctatagata
    10441 caatagtaaa cgaaataaaa tcttctcaaa taataatgtt accaggtgga tttagtgctg
    10501 gtgatgagcc agatggttct ggtaaattta tagctactgt atttagaaat cctagagtgc
    10561 aagaagctat aaatgaattt ttaactcaaa aagatggact aatgttaggt atctgtaatg
    10621 gattccaagt attaattaaa ttagggttag ttccatatgg agaaataaga gttccaagtg
    10681 aaagtgctcc aacacttact tacaataata ttggtcgcca tcaagctaag attgctagaa
    10741 ctagaatttc atctaataaa tctccttggt tagctcaaac taatgttgga gatattcata
    10801 atatagcaat ttctcatgga gaaggtaagt ttgttgccag tgaagatgtt atgagagaat
    10861 taattgctaa tggacaagta gcaactcaat atgttgactt taataatgaa gctacttatg
    10921 atattgagtt taatccaaat ggttcattct atgctgttga aggtataact agtgctgatg
    10981 gtagagtgtt tggtaaaatg ggacattctg aaagaattgg tgaggaagta tataagaata
    11041 ttataggtga aaaagaccaa aagatatttg aatctggagt taaatatttt agataaagct
    11101 ttgatgttta attgatagct agaatatata atttagatat ttagatttaa agagatgtaa
    11161 taatatgatg ataatcattt aatattacat ctctttttta tttatctaag aaataataag
    11221 ttcaacctaa gttaaaatac aagctgtgtt ataattaatt attaagtaaa attgaatatt
    11281 tattggaggg caacataatt aatgaaaata ttaataacag ggtctaatgg tcaattagga
    11341 aaagaattgg ttaaccaatt agaagcaatt aatcaaagta taaaccaacc aaaatatgtt
    11401 atattagcta caacaagaag tgatttggat atatccaatc agactaatgt agataatttt
    11461 attttgcata ataaacctga tgtagttgta aactgtgcag catatacaaa ggttgatgca
    11521 tgtgaagata atatcgaaat tgcatacaaa ataaatgctt taggagtgag aaatctagca
    11581 atagcatcag aaaaagttaa tgctaagctt attcacatat caactgacta tgtattcaat
    11641 ggtttttcta aatatccata tagggaagac aataaaactg aacccaattc tgtatatgga
    11701 aaaagtaaac ttatgggaga aaaatttgtt gaacaattct cacataagta ctttatattg
    11761 agaacagcat ggttatatgg taatgggaat aattttgtaa agaccatgat aaaactttct
    11821 ttagaaaata aagaggttaa tgtggttaat gaccagtttg gaactccaac aagtacagta
    11881 gacttagcaa aaattataat aagaatcatg gaaacagaac attatggagt ttatcatgct
    11941 acatgtgaag gtgagtgtag ttggtatgat tttgccaaaa agatatttaa actaaaaaat
    12001 atagatataa aagtaaatcc aattaagagt aacgaattta aatgtaaggc tccaagacca
    12061 ctatattctg tattagataa ttttatgttg aagttaattg gtttaaattc ttttagaaag
    12121 tgggaagaat ccattgaaga atatttagaa agatatgata taaatgaaaa gtaaatgtat
    12181 tattttgaga aaagaaaata agatattata aaaaatataa atatttcttt tatttatccg
    12241 ataatcataa tatgacaaaa acaaatttaa ggatggaaaa agatgaaagg aattatttta
    12301 gcaggagggt ctggtacaag actatatcct ataacaaaat gtatatcaaa acaaacactt
    12361 ccaatatacg ataaaccaat gatatattac cctatgtcgg ttcttatgtt ggcagggata
    12421 caagatatat taataatatc tacaccaaga gatataaaag catttgaaga attatttgga
    12481 gatggaagtt cactagggat taacataaca tatgcaattc aggagaaagc aaatggaatt
    12541 gcagaagcgt ttataatagg agaagaattt ataggaaatg acagagtgtc tcttgtatta
    12601 ggggataata tattttatgg atatggattt agtgaaaggt tggagaatgc agttaaaaga
    12661 aaaggagcaa ctatatttgg gtatcatgta agtaatccaa attcgtttgg agttgtagaa
    12721 tttgataagg attttaatgt attgtcaata gaagaaaaac ctacagttcc taaatctaat
    12781 tatgctatac caggattata tttttatgat aatgatgttg tagagatagc aaagctagtt
    12841 gaaccgtcag atagaggtga attagaaata acctctataa acagtgaata tctaaaacga
    12901 ggaaaactaa aagtggaact tctaggaaga ggtatggctt ggcttgatac tggtacacat
    12961 aaaggtctat tagatgcttc taattatgtt gaagtggttc aaacaagaca gggattgtat
    13021 atagcatgtt tagaagagat tgcatataca aaaggatata taagtagaga acaattaatg
    13081 aaactagcca agccactgat aaaaactgaa tatggtcaat atctattaaa attggctagt
    13141 gaagatatgt aatatggtag ataaaattta ttataaattt tattaaggga gtttattata
    13201 atgggaaaaa tcaatgttat aaagactaaa atagaaggat tatatatagt cgaaccaaat
    13261 gtattttatg atgaaagagg atattttatg gaaagctata ataaaaagga ttttatagag
    13321 cagggtcttg atatgtgttt tattcaagat aatgagtcta gctcaagaaa aggcgtttta
    13381 agaggaatgc attttcaggt taaatatcct caaggaaaat tagtcagagt tacagaggga
    13441 gaagttttcg atgttgcagt tgatttaagg aaagattcac aaacatttgg gaaatgggaa
    13501 ggtgtaatat tgtctgataa gaataaaaaa caattttata ttccagaagg gtttgcacat
    13561 ggatttttag ttttatcaga aaaagcagta tttagttaca aatgtacaaa ttattattac
    13621 ccagaatttg agaatggttt tatatggaat gataagcaaa tatctattga gtggccttta
    13681 aaaggaattg aagaaatatt tttatcagaa aaggacaaaa aacaaaaaag ttttaaagat
    13741 atttgtacaa gacgttaata aaaatttatt agaattaaca tttaggagaa aaaatatgaa
    13801 aaaaatactg ataactggag gagcaggatt tattgggagt aattttatac attatatgct
    13861 taacaagtat gtagattatt ttattgttaa cctagacttg ttgacttatg ctggtaattt
    13921 agagacatta atagaaatac aagacaattc caattataaa tttgttaaag gagatatttc
    13981 aaatagggag caggtgtata agctatttga agaagaaagg tttgatatag ttgttaactt
    14041 tgccgctgaa tctcatgtag ataggtcagt tgagaatcct gatttattta ttaaaacaaa
    14101 cattatagga acacaagtac ttttagatgc atctataaaa tatggagtta aaaggtatca
    14161 tcaaatatct acagacgaag tgtatggtga tttaccaatt gatagaaagg atttattctt
    14221 tacagaacaa tcttctatca atccaagttc tccatattca gcttctaaag catcagcaga
    14281 tttattggta aattcttatt atagaacata tggattgtta acaactatat ctaggtgttc
    14341 caacaattat gggccatatc attttcctga aaaattgatt cctcttatga ttttaaatgc
    14401 tttggaaaac aaacaattgc cagtatatgg gaatggggaa aatgtaagag attggttaca
    14461 tgtatatgac cattgtactg ctatagactt aataatacat aaaggaaata taggggagat
    14521 ttacaatata ggaggtcata atgaaagaag taatctagaa gtagtaaaag tgatattaaa
    14581 tcttttaggt aaatctgaag agttaatatc ctatgtaaat gatagacctg gtcatgattt
    14641 aagatatgct attgatgcaa ctaaaataga gaatgagtta ggttggaagg caaaatatga
    14701 ctttgatttg ggtataaaag aaactgttaa gtggtatata gaaaatgaat cttggtggaa
    14761 agctgtttta tcagtataac tttaaaattt agaaaggggc gttttatatt gaatgagata
    14821 agttcactaa ttaatatatt agcattgact caattatctg gtagtaatac tacaaatcaa
    14881 tgtaattgta catataataa ttcatctgga tttgatatga ttatgatgac cttgttaaaa
    14941 gcattatcac aaaataatca acatacttct aatagctaca atttattgtc taattctgag
    15001 aatttattta atgaacttga taatgtagta aataatacca ctagtagatt tatagatgtt
    15061 gatacaaaag ataaaaatgt aaagttgaga attgaaaatg ctgtagatca agcatcaaaa
    15121 aaatataacg tagattcaaa tcttataaaa gcaataatta aagttgaatc agattttaat
    15181 ccaaataccg tttcttctgc tggtgctaag ggtcttatgc aacttatgcc agaaaactgc
    15241 agagacttag gagttacaga cccttttaat atagaacaga atatagatgc tggaactaga
    15301 catattaagg aatacattga tatgtttgga gggagcatag aaatgggatt gatggcatat
    15361 aatggtggtc ctggaagaat gaaaagtaga ggagtagaat caatctctga cctgtataaa
    15421 atgccaaaag aaacacaaaa ttatattcct aaggttatga aatattatag agggtaggct
    15481 ttattatggg aataaatata gataagctaa acataagtcc aagtataaat atggaggtta
    15541 aaaaactaaa agagagtaat aaattttctg acaaattaaa ctctttaaaa caatcttctt
    15601 cattggaaga tataaaacga aatttggaaa gtgtaaaaaa ggcaggagaa aggcttgtac
    15661 ttactcaaaa ctatggcgat attagtatat ataaaaatgc agtaaaagaa tacctaaaat
    15721 ctgttgtaga taatatgtat tcacttgata aaaatagtag cttttgggag catcagtatt
    15781 ataagaatgt aaagattgtt gatagtaaac ttgaggatat gactagtaag ttaattagtg
    15841 aagaaaaaga aaacatagat attgtttcta ctgttgacat gatacaaggc ttattgatag
    15901 atatgtatag ataaggttat acaaggagaa aaattttatg aatagcaaaa aagaggatat
    15961 atatgaattg aattttgggc aattaataga agaaaaatct ggtgttatag aagaaaatca
    16021 aaatgttttt aatgcaaaag taaatgtagc tgtttctata ggaaatacaa cagaaacaat
    16081 aaaaaatata ttagatttaa aagaaggctc tataataaaa ttaagtaaga atgtggatga
    16141 aaaattagat atatatgcaa acgatagatt gtttgcttat ggagaaagca ttattacaaa
    16201 tgagaggtta tctgtaagat tatctaaaat agaagatcca gatgtaaatt agcattttaa
    16261 atagtatccc tttaggctat tctgtatgag tttagctaag gggatatttt tataaaaaat
    16321 ttaaaatatg tgtgaattat ataaatattt ttattttatg agcgataact aaattataag
    16381 tatgtaattt tggaggtgaa actatggata ttaaaaatgt aagttcaaac attgtagtga
    16441 ataattattt aaagaataca tctggtataa aaaaagtgga agaggttaaa aaaattaata
    16501 aaacagacaa gatagagtta tctaaatttg caaaggaaat ggcaagtgtg tcaaatatga
    16561 gtgatgagaa tattagaaat aaaaaagtcg atgaaataaa aaatgcatta gaaaatggaa
    16621 cttacaaaat aaaaccaaaa gaaattgcga aaaaaatgat agaggatatg cgaggataaa
    16681 gtcatgatta cagacttaaa agttataata tatgaagaaa aaaatataat tagtaagatg
    16741 atggaattat taaaagaaca atttgattat atagttgaaa aagacttaga aaatttaaat
    16801 atgttaaatc ctaaactaga agatattagc agggagcttg catccataga aataaagaga
    16861 agacaacttt ttggtgatga tatttcaatg agtgaagttg ttgaaaatag taatgatgga
    16921 tacttaaaag aaatatatac agacttgaaa cacattcttg ctttagctaa aaatcaacaa
    16981 gaatcaaatg attctctaat aaagaaagaa cttattttta cgaaaaaaat gattaatttc
    17041 ataaaaccag ctgataagaa aactacaact tataattctt atggaaatat tagaaaataa
    17101 atattaggag gagaaatatg gcaggattat tttccatttt aaatacagct aattcaggaa
    17161 tgaatgcgca ccaaaaagca atacagacaa tttctcataa tataagtaat ctagatacag
    17221 acggttattc taggcaaagg accgagtttg ccacaaatag tccaatgtat atgcctagtt
    17281 tgagtaattc aattgcaaga ggacaattag ggactggagt acatgtaacg gatgtaacaa
    17341 gagctagaaa tagtttttat gattaccaat ttagggcaga agctcataag tatggtaaga
    17401 cagtttcaaa atatgattat tacaatacta tagaaactat actaaatgaa ccatcagact
    17461 atggaatatc tgcaggtata gatgattttt ttaaaggatg ggagtcatta tcaaaggata
    17521 taaatagtga cagtaaaaaa agtctagtgg tagaaaacgc atctaattta gcaactttaa
    17581 taagtgaaag ttataagaag cttgataagt taaaagaaga tatagattct aatatagaca
    17641 ctgagataaa acaaataaat gacatgttaa aaagtcttga agatttaaat aaatctattg
    17701 atattatatc tggttctggc tctacaccaa atgatttact tgatgaaaga gatagaattt
    17761 tagataactt gagttttaaa ctagactttg aaaatagtga tgttaaaaat atgcttgcag
    17821 atggtaaatt agaacttaat gaattaaaaa atccagatgg aacttggaaa acaggtatat
    17881 cagggacctt acaaggcttg tttgaaatgc atggtaagat tgatacatat aaaagcgatt
    17941 taaaagatgt atctgatgga ttagctaaac agataaatga tgtttataat acgtctgcag
    18001 gaataacagt aacagatttt tttataactt caaatgtaga tggtgaagat attataaaga
    18061 taaatccagc tataaagagt aacccaaatg aattaaagct tactgctgat gaagcttcaa
    18121 aaattgctaa gttaaaggat gaaaaaatag atattggggt ggctggtgga aaagtaagta
    18181 caataagtga ccactataaa gcctttgcag aatcaatcgg tttagattct caaaaagtaa
    18241 atcaagatga agcaaatcaa cgtaaaatta taaataatat tgataactca agaatgagtg
    18301 tttcaggtgt atctttggat gaagaaatga cagagcttat aaaaatacaa cgttcatatc
    18361 aagctagtgc aaaagttatg tcaacagctg tgcaactact ggatgttgta ataaatggaa
    18421 taatttaata gaagggggaa tttataatga gagtcagtac aggtatgatg tctagcagct
    18481 atctaaactc actacaagat aatctacaaa gattggataa ggttaataga caagttaata
    18541 caacaaaaga aataaacaag ctatcagata atccatataa agcaataaag atattaaact
    18601 caaaaagtga aataaagact atggagactt atgttgagaa ttgtaaagat acagggaatt
    18661 ggttagaaac tactgatact gcattagatc agcttggaaa attattatca gacataaaga
    18721 aaactttggt ggcaagtggt gatggtgcat actcagatga tgaattaaag actcttagta
    18781 atgaaactaa tgaaaaaatg aaagaaattg caaatgcttt aaatgcaaca catgaaggga
    18841 agtacatatt ttcaggttca aatacggggg taccacctgt tgaatgtata gataatgctg
    18901 atggttcagt gagtttaaag tttaattcta gtctaaatct taataaatta aatgactctt
    18961 tgagtatgga tatagctcaa ggaataagtg tagattataa cgttaaattg agtagccttg
    19021 gatttgaccc aacaaaaact gacccatttg aagagttaaa taacatatca aaaaaattag
    19081 caaatccaga tgctgatgct ataaaagagc ttacaacaac ttgtttgggc gatatggaac
    19141 aactaataga aaatacagta aatgttaggt ctatatatgg gacaaaagca aatacagtag
    19201 aggctatgaa ggaaaaaaac gatgatagtt tgatacagtt aaaggatatt ttgtcacaaa
    19261 atgaagaaat agattatggt gaaaaacttg ttcaattaaa ggcagcagag ttaacttatc
    19321 aagcttcact acaaacagga ggaaagttat ttaatgtatc tatattagat tacatataat
    19381 aaaataacgc agatatgatg aaggttacat taaaaaaagg tatattaggt tttgaaaatt
    19441 taaaagaata tgagctttta gatatagaag ataatgatta tttaaaagaa tttaattcta
    19501 ctgaagaaga aggtatagga tttgttgttg tatctccatt tgagattatc aatgaatatg
    19561 aaatagtttt aaatcaggaa acaatagaaa aactagaagt gaaatctcca aaggatgttg
    19621 tattatttaa tataattact cttggtcaga catttgaaga atcaactgtg aacatgaaag
    19681 ctcctatagt gataaatata aaaactaatt ttggaatgca aataatttta caggatgata
    19741 aatattcaat aaggcatcca ttattgagag gtgataatgg atgctagtaa tttcaagaaa
    19801 aaaagatgaa gcagtactta taggagaaag catagaagta aaaattgtag gagtagatgg
    19861 aaacaatgta aaactagcta tatcagcgcc taacaatata agtatattaa gaaaagaaat
    19921 atacgaaaaa gtaaagagtg agaatataaa agctacaaat aaaaatataa aaattttaaa
    19981 gtcattaaaa taataagctt ggaggaagta atatatgtat ggagaaaatc cttataattc
    20041 ttataaacaa aatgctgttt ttatggcatc aaaggaacaa ttgttactta tgcttgttga
    20101 tggagcagta aaatatacca aaatagccaa aacagctatc ttagataaaa atacacaaaa
    20161 agcgcataga gagcttataa gagttcagga tatatttaca gaactaatgg ttacattaga
    20221 ccaaaatgca ggtcaatggg caaaggatat gtacagagtt tatgattttg taagatatga
    20281 actttcaaga gctaatatta gaaaagacat tcaaataata gataatgtgt tgcctgtaat
    20341 agaacaaatt aaagatactt ggcatgaagc tgataaaaaa agtaaagaag aaagaagtag
    20401 gtacaaataa tggaattaaa tttagctgaa ttagataagt tatcaaaaga ggaattacta
    20461 ttgatgcttg tagatggaac agtaaaatat accaatatat ccaaagaagc tcttttaaat
    20521 aacgactatt tgaaagcaca taatgaactt gtaagagttc aaaatatatt tacagaacta
    20581 atgactacat tagaccaaga tgcaggtcaa tgggcaaagg atatgtataa agtttatgaa
    20641 tttataaaat ctgaattagc aatagctgat gaaaataaag atattaaaat aattgatgat
    20701 atattgccaa tagtaaaaca aattagagat acatggcatg aagtttacaa tcaactaaaa
    20761 atataattgg atggagggat aaaggtatgt caagtataag tcctataaga gttacaggtc
    20821 tttcaggaaa ttttgatatg gaaggcataa tcgaagctag tatgattaga gacaaggaaa
    20881 aagttgataa agcaaaacaa gaacaacaaa tcgttaaatg gaagcaagaa atatatagaa
    20941 atgttataca agaatcaaaa gatctttatg ataaatatct aagcgtaaat tctcctaata
    21001 gtatagtaag tgaaaaagca tactcttcta caagaataac cagttctgat gaaagtatta
    21061 tagtagcaaa aggctcagct ggtgcagaaa aaataaatta tcaatttgca gtttctcaaa
    21121 tggctgaacc agcaaaattt actattaaat taaattcaag tgaacctatt gttcaacagt
    21181 ttcctccaaa tgctagtgga gcgagttctt taactatagg agatgtaaat ataccaatat
    21241 ctgaacaaga tactacaagt actattgtaa gtaagataaa ctccctttgc gcagataatg
    21301 atataaaggc ttcttatagt gagatgacag gtgaattgat tatttcaaga aaacagactg
    21361 gttcgtcatc agacattaat ttaagagtaa ttggaaatga caatttagct cagcaaattg
    21421 ctaatgataa tggtatcaca tttataaatg atgctggtgg aaacaaagtg gcaaatgtat
    21481 atggaaagaa tctagaagct gatgtaactg atgaacatgg aagagtaact catataagta
    21541 aagaacaaaa ttcatttaat atagataata ttgactataa tgtaaattca aaaggaactg
    21601 caaagttgac ttctgtcact gatactgaag aagctgttaa aaatatgcaa gcatttgtgg
    21661 atgattataa taaactgatg gacaaggtct atggtttagt tactactaaa aaaccaaaag
    21721 attatccgcc tcttacagat gcccaaaaag aagatatgac aactgaagaa atagaaaaat
    21781 gggaaaagaa agctaaagaa ggtatactta gaaatgatga tgagttaaga ggttttgttg
    21841 aagatattca atctgcattt tttggagatg gaaaaaatat tattgcatta agaaaactag
    21901 gtatcaatga aagtgaaaat tacaataaaa aaggtcaaat atcatttaat gcagatactt
    21961 tttcaaaggc tcttatagat gatagtgata aggtatacaa aacactagca ggttattctt
    22021 cgaattatga tgataaggga atgtttgaaa agctaaaaga tattgtatat gaatattctg
    22081 gaagttcaac ttctaaactt cctaaaaaag caggtataga aaaaactgct tctgctagtg
    22141 aaaatgtata ttcaaaacaa attgcagagc aagaaagaaa tataagcagg ttagttgaaa
    22201 aaatgaatga taaagagaaa agactttatg ctaaatattc agccttagaa tctttgttga
    22261 atcagtattc ttcccaaatg aattatttct cacaagcaca gggtaattaa ttatggataa
    22321 attaaaagaa aaattaaatt tatataaaga tatatcttta caaattataa atttgattga
    22381 aaaggaagaa tatataaaga taagtattaa gttaggtgaa agacaagaga ttataaatag
    22441 tgtaagtgaa attgatagaa atgattttat acagctttat aatagaatgg aattaattga
    22501 aattgatagc aggataagag atattttaca aggacaatta ttagaagtaa aaaaagaatt
    22561 acatgagtat aagcttacta aacaagtgaa cacaatgtat tataacttaa atagagaaaa
    22621 agtaaatatt tttaataaaa aagtataaag ttatgtacca tcttgtccga ttatataaat
    22681 gaagaattaa taaaaaagat taaaagtaga aatgacaagg atgtcaacta tactaaggag
    22741 ggtaaaataa tgagagttaa tacaaatgta agtgctttga tagcaaataa ccaaatggga
    22801 agaaacgtaa atgcacaaag taagtctatg gagaagctat cttctggtgt tagaattaag
    22861 agagctgctg acgatgctgc tgggcttgct atatctgaga aaatgagagc tcaaataaaa
    22921 ggtttagacc aagcaggaag aaacgttcaa gatggtatat ctgttgtaca aacagcagaa
    22981 ggtgcattag aagaaactgg taacatacta caaagaatga gaactttatc agtacaatct
    23041 tcaaatgaaa ctaatactgc tgaagaaaga caaaagatag ctgatgagtt actacaatta
    23101 aaagatgaag ttgaaagaat atcaagctca attgagttta atggtaagaa attacttgat
    23161 ggaagttcta cagaaataag attacaagtt ggagcaaact ttggaacaaa tgttgcagga
    23221 acatctaaca acaacaatga aataaaagtt gctctagtaa atacttcaag tataatgagt
    23281 aaagcaggta taacaagttc tacaatagca agcttaaatg cagatggaac ttctggaaca
    23341 aatgcagcta aacaaatggt atctagctta gatgtagctc ttaaagaatt aaatacatca
    23401 agagcaaaat taggggcaca acaaaataga ctagaatcaa ctcaaaacaa cttaaataat
    23461 actatagaaa atgttacagc agctgaatca agaataagag atacagatgt tgcttcagaa
    23521 atggttaact tatctaaaat gaatatatta gttcaagcat cacagtcaat gcttgctcaa
    23581 gctaatcaac aaccacaagg agttttacaa ttattaggat aaaataattt tgtatattgg
    23641 atatagaaga aagggtaagg ctttgcttta ctcctttctt tttttacaaa tatacttttg
    23701 aaaggaaaat ttatatgtta cttagtatag ttatgattgt taaaaatgaa gaaaaaatac
    23761 ttgaaaaaac tttaaaatca ttaaccacat taagaaattc tatagaaagt gaacttataa
    23821 tagtcgatac aggttctaca gatgatacta taaaaatatc taagaagtac acggaaaaag
    23881 tgtattttca taattggaat aatgattttt ctagtatgag aaatatatca atatcctatg
    23941 ctaaaggtga atggttactt atattagatg cagatgaaat actaattgat tcttcaacta
    24001 ttgtaaaatt ttttaaagat ggtcttgata aaaaatttaa ttctgcttca atacaattaa
    24061 aaaatcttta ctcatatgat aaaaaattat atggatattg ttcggtgctt agacttttta
    24121 aaaacgttga ttttagatac catggtaaag tacatgaaca accaatatac aaaaacccta
    24181 tatttaataa tgttgctgat tttgaacatt atggatattt gtttgaagat gaagaaataa
    24241 gattaaataa ggtgaaaaga aatgaaaaac tattatttca agagttaaaa gagaatgata
    24301 attctccata tacaaactat caattaggga aaaattttat tatattagga aaatatcagg
    24361 aggctttata ctatcttgag aaatcttatg aattatattc taaactagaa ttggcacctg
    24421 gttacttgat aacaagcctt gtaaaaacat atttatattt aggaaaacat aagaagtgtg
    24481 aaagattgtg cttaaagtat attaagaaag acatgaataa tattgatata tattattata
    24541 ttgcacaagc acaagtagat ttaggaaagt atgaaaatag tatagattcg tataagagat
    24601 atatttactt gttagataat tatgaaataa gtacacaagc aaatagttta ttttcagaca
    24661 ctgatactgt agggcttaga gatgaggcta ttattacact gataaagatt tattataagc
    24721 ttgaaaaata tgagtttgta atttcagagt ataataacat tgaagatgtg gaaaagaaaa
    24781 agaatgtgta ctttagctta tttatgtcac tatataaatt gaatagattt gaagatataa
    24841 aaaattatta taaagaagtt tcaaattcta aaattgaaaa aaatagtttt tatagaaata
    24901 tagaagaagt tataaataat attaaagagg atgaaaaaga gtatgtatat aaaatccttt
    24961 gtgacattga aggtaattat ggcattttaa atcaaataag gataaataaa tgtgtatcta
    25021 tagataaatg taaagaaata ctcaatgaag aaaaggaaat aatttatgca tatattataa
    25081 aaatagcttt tgaagagaag attgatttac tagatatttt ttatgaaatg gaatatgtat
    25141 ggatagagaa atatctaaaa tatcttttag cattagaaag aagttttaat ttagtactat
    25201 ataagtacat aattagtcgt ccaaatacat cagatatgga aaagataaga gtatataaag
    25261 ctatatctaa agtagtttta gaaagtataa gcttaagtga tgaaaaatat aaggagattt
    25321 tttatcttta tataatgtat tcttataagt acataagata catatatagc aatttaagtg
    25381 attttgaatt aataaaattt gtttgtaatg atttagataa gttcactata gaatttaagc
    25441 atctacttga tattaaaaat aaagtaaata atgctaatga aaaattagag tatatacata
    25501 gtatgaggaa attactaaat gagtatcctt tttataataa aataataaaa atacttatag
    25561 gtgaattaga agaaaatata aaagaaagtg aagaatttaa ggatttaaaa gatagtttta
    25621 ttattagtat agaaaatatg attgaaactg gcaaaataaa tgatgcgaaa ttattggtag
    25681 atgactattc aaaaacattt gtggatgata taaagatttt aaatataaag ggaattttat
    25741 acatgtttga aaataagctt gataaagctg attttatgtt aaaaaaggca ttatcattag
    25801 atgtagaaaa tgaagatact atatataata tagaatactt aaaaagttta agataagtca
    25861 aaaataggag aattgaagaa tgaatatacc aatagtggtt aatgaaaaat ctaaagacat
    25921 aataaaagat tggcaatata catgtacaaa tgaagatata aaatttacaa gcataataat
    25981 attgacatat aatcaaatag aatatactaa attatgtatt gaaagtatta ggaaatttac
    26041 cccaaagaat aaatatgaaa taataataat tgataataac tcttctgatg gaacagttga
    26101 gtggttaaag ggacaatctg atataaaatc tgtgtataat aacaaaaact taggatttcc
    26161 aaaaggatgc aatcaaggta ttgagttagc tactggtgaa aacatattgt tgttaaataa
    26221 tgatgtaata gttactccaa attggctcta taatttgaat aaagctttat ggtctcaaga
    26281 taatataggt gctgtaggac ctattacgaa tgcatgttca tattatcagc aaattgatgt
    26341 tgattataag tcaattgagg acatgtttat atttgcacac aaaataaata aatctaatat
    26401 taacctatgg aaatatagat tgaagttagt tggattttgc atgttaataa aaaaaactgt
    26461 tctggataat gtaggggttt tagatgaatt attcacacct ggaaattttg aagatgatga
    26521 tttatctttt aggatgatag agtcaggata taagttatta ttatgccagg atacttttat
    26581 acaccatttt ggaagtgtgt cttttaaaga aaatcgtaat aaatatacag atataatgta
    26641 tattaataga agaaaatttg aaactaagtg gggatttaat ccagaatata gtggaatgat
    26701 aagaacagat ttgatttcta aaatagatga tagtttggag gcaaatatta atgttttaga
    26761 aataggatgc ggtatagggg ctactctttt agaaataaaa aatatatata aaaatgcatc
    26821 aatatatgga atagaaatta gtgagtcagc aggtaaactt gcaaagtata gtgcagatgt
    26881 acttatagca aatatagaaa aagtaaaatt ggattataaa aaagattttt ttgattatat
    26941 aatattggga gatgtacttg aacatttgat tgacccatgg aagatagtca atgaattgaa
    27001 acaatatcta aagaaggatg gttatctggt atctagtata ccaaatatta tgcatgtttc
    27061 tgttttaaga aatcttgtgt taggtaattt tacatatgaa gattcaggaa ttttggacaa
    27121 gactcattta aggtttttta cattaaatga gataacaaaa atgtttaatg atagtaatta
    27181 cataattaat agtattacag ctactagtat gccactttcg gaaattgata atagcttcat
    27241 tgatacaata tgtaaggaaa gctgtaaaga aatgagacaa cagtacgaat cttatcaata
    27301 tatagtaaaa gcctctaaaa tcctagatac taaaagatat tctagtatta gtatgattaa
    27361 tttaaaatac gctttaatga gaattgataa tgaattagat gtagatagta atttaagtta
    27421 tatatttgat aattataata taactgaacc atgttttgtt gatgatattg aatatctaat
    27481 tgatacattt gtgataaata aagcaactct attaaataga ttaggagttg aggcatttaa
    27541 cagaggtcta ggagatttta gtataaacat gtttatgaca gtattagaga tggatagaga
    27601 tgatattgac tcaatatata atataacaag tgtattatgt gaattagaag aatttgatac
    27661 tgcttataat ttaattaaaa atagtagcga aaaagtacag agtgaaagtg atatacaaga
    27721 gatactttgt tttattgagg ggaagaaata tgaataatga taagatatgt tttataacct
    27781 gtgtaaatga tgaaaggctt tatgaagaat gtcttgtata tataaatagc ctagacattc
    27841 ctaaaaactt tagtatagaa acagtagctt taagaggagc cgattctatt gcctctgctt
    27901 ataataaagc tatgagagaa acagattcta agtataaggt atacttacat caagatactt
    27961 ttataattaa taaaaatttc atacatgata ttttagagat atttaaaaaa aatacagatg
    28021 tcggaatgat tggtgtagct ggagtaaaag atatgcctgt gaatggaatt tggtgggatt
    28081 ctgataaaaa agcagggaaa gtatatgata gtcatacagg taaaatggat atttatgaat
    28141 ttagtaactt tgaagaatgt tttaatgaag ttatggctat agatggatta attatgatta
    28201 ctcaatatga tgtattttgg agagaggata agtttgatgg gtggcatttt tatgatttgt
    28261 cacagagtat ggagtttaga caaaataaat acaaagttat tgtaccaaaa caaaataagc
    28321 cttggtgtat gcatgattct ggagtagtaa atataaataa tggatatgat gaatatagga
    28381 taaaatttgt aaaagagtac ataaatcttg atttatatag agaaaatttt tatgaatttg
    28441 ataagaagaa agcaagtacc atacttaaga gtaataagca tgtaatggat tgtatgaaag
    28501 aggagcttac agaacaatta agattaaaga attacaagga tgttttaggt attgtttcta
    28561 aaattgctca tttttctgtt tttaatcata ctggtttgta ttcttgtgaa gaatgtgaga
    28621 gagcattgct agaatgtgca aataatttac cagaaataga aaatacgaaa ttatttacaa
    28681 aaaaagattt aaaaagaaga aaagtattac atgttttaag tgaaggatat tcaacaggag
    28741 gtcatacaag actagttaaa aattggattg aaattgacaa agactctgtt cattcagttg
    28801 taactacatg gcaatcagat acactgccag actggttatt aaaagcaata aatgatagtg
    28861 gtggcgaagt tatatctttg gatttaattt cagatgatta tttaaaaaga gctgtagctt
    28921 tgaggaaaat agcttatgac tgggctgata tagttatttt atatgttcat atgtgggata
    28981 caatacctgt tgttgctttt ggagttgatg ggaaaatacc tattttacat gttaatcact
    29041 atgatagtac tttttggtta gggacaagta ttgcagatat tgtcattgat tttagcgaaa
    29101 ctagtcaatt aattacatta gataaaagag gcatagataa atctgaaata ttaccaatgc
    29161 cattaaaaga aaaagaagta ttttcaaaat ctgaagttag aaaacgatat aatttagagg
    29221 atgatgtgac aataatattg actattgcta gtgaaagaaa atacaatagt gtaaatgata
    29281 ttaattactt taaaattgct aaaacattga ttgaaaatac agataataca attattattg
    29341 ttgtaggagt tgatacagag aatctctatt ggaaaacttt aagtaatgtt acagataata
    29401 gagttatatg ttttggaaca acagcaaata tagatgattt ttataagatt tcggatatat
    29461 acctagagtc tttcatggta ggaagtcata cttcaaaatt agatgcaatt ttatatgatt
    29521 taattcctgt taagtttaaa aatgaagttt caccaatttt tacaaatatg tggaatttta
    29581 tagataagtt tgattataag aatataaaag aggttataga catgataaga aggtacaata
    29641 ctagtgaatt tgacagggat aagataaaag caaaacaatc ggaaataaaa cattttgtta
    29701 tagataaaca ttgtattgaa ataaaaaact atatagacaa aatatataat gaagttaatc
    29761 atcataaagt taagtatgat ttatataaaa atgatgaaat cgaggactat cagctttttg
    29821 tagcattata tggctatatg gtaaaaaata aaaacatgtc ataggtggta aaatgaacaa
    29881 tattcatatc aataaatttg aaaaaagctg tgaaaaaaac tgggattatt ttgtagaaaa
    29941 taaatctata aatggtacat ttctgcaaac tagaaatttc cttaattatc atgaggaggg
    30001 aaaattcact gaccactcat taataatatg taaaggtgaa gaaatactag cagtcgtacc
    30061 tgcatgtgaa acttatgaag atggtgaaag aatatttttt tctcatttag ggagtacttt
    30121 tggtggcatt ataataagaa gtggttttaa tgatattagg catgtaaatg ccattacaaa
    30181 taagttagaa atatatttac aggaacaaaa tttcgataaa gtatatttaa aaaatacatc
    30241 aagaatattt tgtaaagagg atacttcatt actagaatat tttttattta agaataatta
    30301 catgttttat gatgaattaa gtatgttttt agaattaagt aagtataaag aagatatatt
    30361 agccaatttt aaatacagta aaagaagaga ttttaaatat tctttgaaga ataatcttac
    30421 ctttaaaaga ctgtatagtg atgaagatat agtttgtttt tttaatttac tttctaattg
    30481 tttagatagt aaaggagcta aagtaataca ttcagtagaa gaacttttgg agtttaaaaa
    30541 taatagatta aaagatattg tagaatttta tgcagtatat attgatgaca ttatgatttg
    30601 tgggagcatg gtatttaact ttcaagaaag agtttatcat actcaatatc tagcatcaaa
    30661 ttcgacatat aataaatatt atccaatgat atttttatac tataatctaa taaaagatgc
    30721 tttagagaga aattttaagt atctttcttt tggaattagc actctaaata gaggcaaata
    30781 cttagatgag ggtttagcta catttaaaga aggttttggt tcaagtgcaa tttgtaacag
    30841 aacatactat aaaaatataa ataagtgaga atcatgtttt atagagtttt tagagtatag
    30901 taaatatcta gtactataat attacataaa aataattagt ttataaagaa ggtttgtata
    30961 tgaaaagatt aatgatttta ggctcaatta agtattttga gaatattgtt atgtcagcta
    31021 aaagagaggg aatatacaca attgtttgtg ataatagaat tgatactcct gcgaaaaaga
    31081 tttgtgatga agcaatagat gttgatgtat ttgattttga taaaataaag aatatagcta
    31141 tagataaaaa gatagatgga attttaactg gatttacaga ttctttaatg aagccatatg
    31201 tttatattgc aaatgaacta aatttgccat gtgtgatttc ttgcaatcaa cttgaatctg
    31261 taactaataa ggctgttatg aaaaattatt ttaaaaataa tggaatacct acaactgatt
    31321 attgtattat tgaaagtttg aaagatttag aaaaaataca aggattaaat tttccacttg
    31381 taataaaacc tgttgatggt tatggttcaa gaggtgtata ttttgtatca aacatagaag
    31441 aattaataga aaaatttgaa tattctaagg aagcctcgca agatggacaa gtaataattg
    31501 aggaatttta tgaaagtgag gaaatacaag gactagcttg ggttcataat ggagagtcac
    31561 atgtatttta tattggagat agagagcttg taaatataca tacaggaaga gctggaaaac
    31621 cagatagatt aatttatcca tcaaagtatt gttttcaata tgaagaagaa ataaaatcca
    31681 tatatcaaaa tataacgcag gcttttggca taaaaaatgg acctctttat gtacaaatgt
    31741 tagtgggagc atatggaata aaggtatctg aagtaatgcc aagattacct ggaggttgtg
    31801 actatatggc tatttctcaa ataacagatt tagacatagg aagtttattt gcaaacttta
    31861 gtgtgaataa tgaagtaaat tttgatgaaa taaagaaata taatatgcat ctatcaaaat
    31921 gtgtatatgc attaccaata tacataagac ctggcacaat aaaaaagata aataatatag
    31981 ataaaataga aaacttagat tatgtcactc aatttctatt actagtaaaa gaaggagata
    32041 caataggatt atatgaagat gagagacagg attgtggaag agtttatgga gtagcaaata
    32101 atgtatttga tgcaaatata aagaagaaat gtattcatga aatgatagaa atacttgatg
    32161 aaaataatga aaatatgata gaaaattttt aaataaggga gtatgataga tatgaagcta
    32221 ataactcatg aacaaattat gtcattaaac atttctcaga aacaaggata cgagtggata
    32281 aaagaatcct ttttaacaaa aaaagattgt caattaccac caaaaataag catggtagaa
    32341 gaagggcatg tattttacaa tgtaatgccg tccctaaatc ataatcataa gattggtgga
    32401 ataaaaatgg ttacaagatt gccagggaga aatccaagtt taaattcaga gatatttcta
    32461 tataatatgg aagatgctaa attaaaggct attttagatg gcaattatat tacagctttt
    32521 aggactgctg ctgttgctgc cttggctatc gatacatttg ctaaaaaaga ttttaatact
    32581 attgctatta tgggtttagg tgtaactgct tctgctataa tgaatatgct ttcatatact
    32641 cttaaagata aaaatataaa tataaagtta cttaaatata aagaccaagc agagtcgttt
    32701 gtaaaaaagt atgaaaaaaa tgaacaattt agttttgaaa tatgtgacac acatgaagaa
    32761 cttattgtag attcagatgt agttatttct actgtgacat atacagatga atatttatca
    32821 gaatttaaat ggtttaaaaa aggtgcttta ttattacctg tacatgttat gggatttgga
    32881 aatgttgatt atctatttga taaagtatat ttggatgatt ataatcattt gaaacatcta
    32941 gaaaatatag atagatataa atattcttgt gaattttatg atgttttaag taaacaagct
    33001 aaaggaagag aaaatgatga tgagagaata atatcttaca atataggttt gtcaatacat
    33061 gatatattat ttgcaaataa aatttacgaa ttgtgtgaaa cactaagctg tgaagaaata
    33121 gaattaaaag aaccaaaaga aaaatattgg atatagttta tttcattcat atttacaata
    33181 gtttattgta aatatgaata tgaagtaata tattgttaga aaattagcat gaataggata
    33241 gataaattta tggataaata taatttaatt aggttcgtat ctattggaaa cgaatatata
    33301 ggaaatttag ttgcattaga atcaaataaa gcaatacctt ttgatataaa gagagtttat
    33361 tacatatatg atgtaccaaa caatgtaaag agaggatttc atgcacacaa aaacttggaa
    33421 caagtactag tttgcctaaa tggaagtgtg aagataaaat gttatgatgg tgtaaaagag
    33481 actatattta atttacataa aaatgatgtt ggattatata taggaacggg agtgtggcat
    33541 gagatgtttg attttgatga aaaaactgtt ttaatttcta tagcatctga acattataat
    33601 gaagaagatt atattagaga ctataaagaa tttttaaagt atttaaataa tatgtagtta
    33661 ttatacagtt agtgatgtat tttcttaatt aaataaattt aagatagcca catagagaat
    33721 agatagatta ggatgtgaat ttattgttgg ttaaatttaa taatttatta aaacagttcc
    33781 aaacccataa agaagaatat gaacagaaga ttaaggatgt aatggaaagt ggatattata
    33841 tccttggaaa tgaagtaaag cattttgaag aagaatttgc taactatgta ggtagcaaat
    33901 attgtgttgg tttagccagt ggtttagatg cactgataat tgcttttaga gcattaggaa
    33961 taagcgaagg tgatgaagta atcgtgcaat caaatacata tattgctagt gttatgggaa
    34021 tcagtataaa tggagcaaca ccagtatttg tagaaccaaa tgaatattat aatatagatt
    34081 catctaagat agaagaaaag ataaatagca aaacaaaagc tatattagta gtgcatttat
    34141 atggtcaagc aagtaatatg aaagatatac aagaattatg tgataagtat aacttgtatt
    34201 taattgaaga ttgtgctcaa tctcatggag ctaaatatga tggaaaaatg acaggaacat
    34261 ttggtgatat aggttgtttt agtttttatc caagtaaaaa tttgggtgca tttggagatg
    34321 caggagctat agtaactagt aatgaggaaa tagctgataa agtgaaaatt cttagaaact
    34381 atggctcaga aaagcgatat tataataaat ttgtcggata taattcaaga ttggacgaaa
    34441 ttcaggcagg tttattaaga gtaaaactta aatatctaaa tgaaattgaa attgaaaaat
    34501 caaaaatcgt agatagatat cttaatgaaa ttaataatcc caaagtcata cttccaaaga
    34561 tagaaaataa ttgtactcat gtatggcatt tatttgtttt acaagttgaa aaacgagata
    34621 catttatgga atacttaaag aaaaatggta tagagtctat aatacattat ccgataccac
    34681 cacatctatc tgaagcatat gcttatttgg gatataaaaa ggggagtttt aatatagctg
    34741 agaactattc agataaagtt gttagtatac ctttatttat cggtatggaa gatgaagaag
    34801 ttagtt
//
LOCUS       DAGSWI010000029        34697 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.029, whole genome shotgun sequence.
ACCESSION   DAGSWI010000029 DAGSWI010000000
VERSION     DAGSWI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.029"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(117..773)
                     /locus_tag="MJF80_001274"
     CDS             complement(117..773)
                     /locus_tag="MJF80_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBZ0279946.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILAEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(828..1457)
                     /locus_tag="MJF80_001275"
     CDS             complement(828..1457)
                     /locus_tag="MJF80_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBZ0279947.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(1588..2229)
                     /locus_tag="MJF80_001276"
     CDS             complement(1588..2229)
                     /locus_tag="MJF80_001276"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBZ0279948.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(2238..2909)
                     /locus_tag="MJF80_001277"
     CDS             complement(2238..2909)
                     /locus_tag="MJF80_001277"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBZ0279949.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANIIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(2914..3798)
                     /gene="rsgA"
                     /locus_tag="MJF80_001278"
     CDS             complement(2914..3798)
                     /gene="rsgA"
                     /locus_tag="MJF80_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBZ0279950.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLVLEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(3896..4633)
                     /locus_tag="MJF80_001279"
     CDS             complement(3896..4633)
                     /locus_tag="MJF80_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBZ0279951.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(4741..6756)
                     /gene="pknB"
                     /locus_tag="MJF80_001280"
     CDS             complement(4741..6756)
                     /gene="pknB"
                     /locus_tag="MJF80_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBZ0279952.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(6773..7525)
                     /locus_tag="MJF80_001281"
     CDS             complement(6773..7525)
                     /locus_tag="MJF80_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBZ0279953.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(7545..8576)
                     /gene="rlmN"
                     /locus_tag="MJF80_001282"
     CDS             complement(7545..8576)
                     /gene="rlmN"
                     /locus_tag="MJF80_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBZ0279954.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(8584..9909)
                     /gene="rsmB"
                     /locus_tag="MJF80_001283"
     CDS             complement(8584..9909)
                     /gene="rsmB"
                     /locus_tag="MJF80_001283"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBZ0279955.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFILDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQGVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(9971..10672)
                     /locus_tag="MJF80_001284"
     CDS             complement(9971..10672)
                     /locus_tag="MJF80_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBZ0279956.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(10741..11493)
                     /locus_tag="MJF80_001285"
     CDS             complement(10741..11493)
                     /locus_tag="MJF80_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBZ0279957.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            complement(11503..12432)
                     /locus_tag="MJF80_001286"
     CDS             complement(11503..12432)
                     /locus_tag="MJF80_001286"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBZ0279958.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(12447..12887)
                     /gene="def"
                     /locus_tag="MJF80_001287"
     CDS             complement(12447..12887)
                     /gene="def"
                     /locus_tag="MJF80_001287"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBZ0279959.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEDLIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(12905..15430)
                     /gene="priA"
                     /locus_tag="MJF80_001288"
     CDS             complement(12905..15430)
                     /gene="priA"
                     /locus_tag="MJF80_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBZ0279960.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(15669..16868)
                     /gene="coaBC"
                     /locus_tag="MJF80_001289"
     CDS             complement(15669..16868)
                     /gene="coaBC"
                     /locus_tag="MJF80_001289"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBZ0279961.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(16873..17139)
                     /locus_tag="MJF80_001290"
     CDS             complement(16873..17139)
                     /locus_tag="MJF80_001290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBZ0279962.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(17142..17759)
                     /gene="gmk"
                     /locus_tag="MJF80_001291"
     CDS             complement(17142..17759)
                     /gene="gmk"
                     /locus_tag="MJF80_001291"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBZ0279963.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(17789..18670)
                     /locus_tag="MJF80_001292"
     CDS             complement(17789..18670)
                     /locus_tag="MJF80_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBZ0279964.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(18674..19504)
                     /locus_tag="MJF80_001293"
     CDS             complement(18674..19504)
                     /locus_tag="MJF80_001293"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBZ0279965.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(19791..20825)
                     /gene="ltaE"
                     /locus_tag="MJF80_001294"
     CDS             complement(19791..20825)
                     /gene="ltaE"
                     /locus_tag="MJF80_001294"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBZ0279966.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALNLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            21121..22896
                     /locus_tag="MJF80_001295"
     CDS             21121..22896
                     /locus_tag="MJF80_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBZ0279967.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(23111..24763)
                     /gene="abc-f"
                     /locus_tag="MJF80_001296"
     CDS             complement(23111..24763)
                     /gene="abc-f"
                     /locus_tag="MJF80_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HBZ0279968.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENRIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(25062..26273)
                     /locus_tag="MJF80_001297"
     CDS             complement(25062..26273)
                     /locus_tag="MJF80_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBZ0279969.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(26557..27090)
                     /gene="pyrR"
                     /locus_tag="MJF80_001298"
     CDS             complement(26557..27090)
                     /gene="pyrR"
                     /locus_tag="MJF80_001298"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBZ0279970.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(27450..28397)
                     /locus_tag="MJF80_001299"
     CDS             complement(27450..28397)
                     /locus_tag="MJF80_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBZ0279971.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALFINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(28366..28812)
                     /gene="lspA"
                     /locus_tag="MJF80_001300"
     CDS             complement(28366..28812)
                     /gene="lspA"
                     /locus_tag="MJF80_001300"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBZ0279972.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(29122..29901)
                     /locus_tag="MJF80_001301"
     CDS             complement(29122..29901)
                     /locus_tag="MJF80_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBZ0279973.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(29956..30438)
                     /locus_tag="MJF80_001302"
     CDS             complement(29956..30438)
                     /locus_tag="MJF80_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBZ0279974.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(30704..32125)
                     /locus_tag="MJF80_001303"
     CDS             complement(30704..32125)
                     /locus_tag="MJF80_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBZ0279975.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKQQNNHKLAS"
     gene            complement(32271..33041)
                     /locus_tag="MJF80_001304"
     CDS             complement(32271..33041)
                     /locus_tag="MJF80_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0279976.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELSNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(33046..34248)
                     /locus_tag="MJF80_001305"
     CDS             complement(33046..34248)
                     /locus_tag="MJF80_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBZ0279977.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT        11047 a         6361 c         3480 g        13809 t
ORIGIN      
        1 tcaatgattt gaaatataaa attaatatca ataaaaccta ctttttaatt ttttaaatat
       61 ttatttttaa tttattttgt ttttaattta cttcgatttt aaaatgtcct tgacttttat
      121 tttataaatg tacatatttc ttctactggt tttttagatg gactcttcaa attgtcctct
      181 ggcactccaa gagctaacat tgcacctaaa aaatactctc ccttttcaaa accagtttct
      241 gcttctaaaa cagcttctat ctcatctgct gcatagtttt gacttgtcaa ccaacaagaa
      301 ccatatccaa gttcaattgc tgatagtgta aagttttcaa ttgcagcacc tagactctgc
      361 atacctggat ttcttataaa taatttatct atagtctcct taggtgcatc tataagctct
      421 aattcataat atcctgatgg gttatatacc tttgtaaaaa ctaatactaa aactggagct
      481 tttaaataaa atagtgtaaa gtttttaaca aattttctaa atctattagc cttatccact
      541 gatactttat ccatttctgc aagtatttct tgttgctttt tagttatcac atctgctatc
      601 ttttccatta aatcacggcg ttttataaca acaaaatgcc agttttgaat attcttgcct
      661 gatggtgctg cacctgctgc ttttatcatt tttaaaatat cttcatctga tacatcttga
      721 tttttaaact ttcttacact ttgtctttta aaaatagtat cttgtaattc catttttaaa
      781 tccccccata acttttttat tattttaata taatttttaa actcatctta ttttaatata
      841 tcatgttcta acaaatcttt aaatgagtct ataaaataat catatacatc tacattccca
      901 aacccatatt tggcatatat aaaaggtata cctacaaact tagcactatt tgcatcacct
      961 tgcgtatcac caacatagat tggatttatt aatttatttc tttcaattat aagtttatta
     1021 ttttctcctt tatgcaaacc agtattacca ggacattcaa aatctataaa atactgttct
     1081 agtttatgtg cttttaaaaa agattctata tatccagctt gacaattact tactataaat
     1141 aatttatatt ttttagatag taatttaaga gtatcttcta tatcatcaaa taatttagct
     1201 ccattttcag ataaatagtc acattctttc aaagaacatt cattcaataa attcattctt
     1261 tcttcttcac ttaaatttgg aaataaacga tttgctattt cctcaaaaga aagccccata
     1321 agacttctaa ataaatcttt tgtgacactt aattctattt ccttatgttt acttaaaaca
     1381 tcctgccaga ctttgcatac ttcttcagtt gaatcccaca atgttccatc taaatcaaaa
     1441 ataatactat ctaccacaaa aaatccccct tatgtcaaag tttattatat atttataatt
     1501 atatactaaa aagcagatac aatcataaat atattttact tgattgtatc tgctctacaa
     1561 tataaaacca attgtcaaaa tcaaatttta taaatttcta actacaagta cacagccttg
     1621 ctctacttta atcttacaag aattactcaa cataacattt gatattccaa taggtacaga
     1681 gtatttcata taataattat cgagagggta ttctaagcca gtaagtgtta cacccttagc
     1741 atcaccattt atagctatta cagaaatagt gtcccctggg tttccataca atgaaatttc
     1801 ctcattttca actatataca tctcttcttt atcagaaagt atcctagaat atataccatc
     1861 ttcttttaaa taatataata gctttatatt tgcaagagtg tggtcaatcc ttccccctaa
     1921 agcaccaaaa aaatcaatat gatttgcttt aagtgtctta gctaaaaaaa gacaaagctc
     1981 tgtatcagtc tcatcttttt tagatggaaa tttttcaaat ttaacacctt tatttttata
     2041 gaaatttatc ttttcttctt ctactgaatc taaatctcct aatatataat ctggcattat
     2101 ttccatttta taagtgtgat ttgcccctcc atctgcacat attatgcagt cataacattc
     2161 cttaattata atatctcttg taacttcgta atcttcaatt tcgccattta aaacaataca
     2221 gattttcatt attaaattta aactaattct tcaagagaag cattctttct aaataaatta
     2281 actgtttctt gaatgtcatc acttccaaat attgcagaac ctgctactat tatattagct
     2341 cctgcttgta caacttcggc tacgttttta gggcttatac caccatcaac ttctatatca
     2401 atgtttaatc cttgactatc tataatagct tttaactctt taatcttatc taaaacacaa
     2461 ggaataaatg attgtcctcc aaatccaggg ttaacagaca ttaataatac catatctaaa
     2521 tcaggtagta catgtttaat cgtatctact ggtgtagctg gattaagtac tacaccagct
     2581 tttattccat gtgattttat attttgtata gttctatgta gatgaacaca agcctcctgg
     2641 tgaacagtta ttatatcaca tcctgctttt acaaattctt caatatactg gtctggattt
     2701 tctatcataa gatgagcatc aaataccata tttatatttt ctttctttaa acttttgact
     2761 acaagtggtc ctaatgtaat gtttggtaca aagtgtccat ccattacatc gatatgaaga
     2821 tattcacatc ctgcactttc tacttttctt acatcttcta ataattttgc aaaatctgct
     2881 gataatattg atggtgctaa tttaatcatg tttctaatac ctcctactat tattttgtct
     2941 tatttcatgt agcagctgta tataactatc atatctttct ttagatattt ctccatttgt
     3001 aacagcttct tttatagcac aattaggttc attttcatga atacactttg aaccaaactt
     3061 acaatcatta aatttatcaa attctataaa ataatcttta agttcaactt cctctatatc
     3121 ttcaaggaca agggaactaa aacctggtgt atctgcaacc attccaccaa attctaattt
     3181 taaaagttca gcgtgacgag tagtatgttt tccccttttt attttatcac ttacaactcc
     3241 tgtttgaagt ttaaaatttt catctatttc atttaataag cttgattttc caactccaga
     3301 tggtcctgca aacactacta cattttcttt taattcttct tttactttat ctatatttaa
     3361 cttagtaata ttacttacag gaatgacttt atagccacta agttcatata tatttttaac
     3421 agtttctaat gtatcattat catctaaatc tgcttttgta aggattatta cagtttctaa
     3481 attttctttt tctgccaaaa ctataaatct atctaaaaga gataagtttg gtttaggatt
     3541 ttttattgcg aatactatta atgctttatc tacatttgca ataggaggtc ttataagttc
     3601 tgtatctcta ctgtcgattt cttctaagat accctttttt tcatcttcat caacaatgct
     3661 tattttgact ctatcaccaa ccaaaggtgt tattttctgc tttctaaata tccctctagc
     3721 cttacattca taaataccat tataagtatc tacataataa aatccactaa tcccctttat
     3781 aatttttcct tctagcatca aatgcctccc tttataaaat ttatataaac acatactgtt
     3841 tatgagtagt atatcgtgtt attctttcct atttttatat aataccacat tttttttaaa
     3901 taacaaatcc tccgtttgga ctaattacct gaccagtgat aaaatttgat ttgtctgatg
     3961 ccaagaaaat agcacaattt gctatatctt ctggagttcc tagcctcatt agaggggttt
     4021 cttccacaag agcgtctata tcttcctcat tatatccaga taacatgtca gtattaatga
     4081 cacctggagc tatgctattt actctaatat ttgatggtcc tacttcttta gctaatgcct
     4141 ttgtcatacc tattatgcct gcttttgtta tagaataatg cacttcacaa gaggcacctg
     4201 atatacccca gattgaagat atattaatta tatttccttt tttttctgat atcatgtatt
     4261 ttaaagcaac ttgtgaacaa taaaagcttc cttttaaatt aatattcatc atattatccc
     4321 aatcttcatc agttatatct gtaaacaact tatcttgact tacaccagca ttgtttacaa
     4381 gtacatccaa tcctccaaat tcttttatac aataatctac catatcctct acatcttctc
     4441 tatttgaaat attagcctta aaaagtttta cagaaaaatt tttttcattt aatatagtgt
     4501 acaactcttt agcttcattt tctgacttat taaaatttac taatacattg taaccttctt
     4561 ttgcaaaagc tttagacata gctttaccaa tccctctagc tcctcctgta attaaaactg
     4621 ttttcttctt cataaatact tttctcctta tattataacc aattcagtat tttatacaaa
     4681 acaaactgcc ctttaaagaa aatattttct ataaaaggac agctcttatt ttattaatat
     4741 ttattctgtt ttaccaatgt ttgcgccagt tcctgatgaa gttcctccat tatctcctga
     4801 gtttgagcct cctgaactat ctccattgtc tcctgagttt gaacctcctg aacttcctcc
     4861 attgtctcct gagtttgagc ctcctgaact tcctccatta tctcctgagt ttgaacctcc
     4921 tgagtttcca ccactatttc ctgagtttga acctcctgag ttacttccat tattgcctga
     4981 gtttccacca ctatttcctg aatttgagcc tcctgaattt ccaccactgt ttcctgagtt
     5041 tgaaccaggt tctgttggtg tattattctc tggtgttgtt ggttttttat ctggtactgt
     5101 tggagtctgt gtatttgatt tttctgaacc tttgcttaca tataggttta cctcatcacc
     5161 ttcctgagct cttgatgagc cagcacctgg actctgactt aaaactgtac cttctttata
     5221 tattgagctg tattcatact taacagttcc cacttttaag ttattttctt caataagttg
     5281 ttttgcatta ctcaaagtaa gtcccactac attaggaaca gatgcctgac ttgaaccttt
     5341 acttacgaca acatttatag tatcaccctt tttgacattt gtacttccac cttcaggtgt
     5401 ctgtgatatt atacaatttt catcaacttc actattatac tcttcacttt ttacactcag
     5461 gtataatcct tctttttcaa cagcactttg agcttcttct aatgtcatat ttacaagatt
     5521 aggaacagtc aaagatttat tgccaaaccc tccagcaaat aaaaacttat atgctaagaa
     5581 tacttgagca cacaatatca gtatcaaaac agccgcaatt acttttaatc ttcttctaga
     5641 ttttgggctt tcctgttttt tcttcttttt cttagttctc ttacttggag tactcttagg
     5701 tctctgattc tttttagctc tcattatctc ttcctcttct tcatcttcat catcatcttc
     5761 ttcataaaaa tcatcatagt aatcttcatc atcatccaaa tcagctactt caacaggctt
     5821 aaccactttt tcaggagctg gttttgcaag tgttggattt acaactttat taatttcctt
     5881 ttcatcaatt ttcttagttg caaaatcatc atattctttt ataaaatcta aatcaatatt
     5941 tttttctata tactctatat cttctatcaa ttcttcagca gtttgatatc tatctgcact
     6001 agatttttct gtaagctttt ttattgtagt tcttacactt tgaggaatcc taactttttc
     6061 ttctgatgta aagtctatat catcattaat gtgttgaagt gctatagaaa ttggactatc
     6121 tcccctaaat ggaacttttc ctatcaacat ttcatataaa actataccta gagaatacag
     6181 gtcagcatta ttagttacaa atttaccttt agcttgctct ggtgaaaaat aatgcactga
     6241 acctattata ctaccaatat tagtcattgt tgaatttgaa acagctttgg ctataccaaa
     6301 atcagccact tttactactc ttccttcatt tgatatgaga atattatgag gttttatatc
     6361 tctatgtata atcccctttt tgtgcgctgc actaagtgcc atagctattt gttttgttat
     6421 atctaatgca gtatattcat ctaatatacc ttcatcttgt ataatctctt ttagattctt
     6481 cccatctaca aactccatta caatgtagtg aacttttcca tcttcaccaa catcataaac
     6541 atttactata tttggatgtg aaagacttgc tactgcttct gcctctcttt taaactttgt
     6601 tagaaattca tcatcatcta caaattcagg tctaagtact ttaagtgcaa cagtcctgtt
     6661 taataatctg tctttagcct catatacaaa ggccattcct ccatcaccaa tcttcctgat
     6721 gatttcatat cgatttccta aaattgtatc tcccacaata tcaccgtcct aattatactt
     6781 ttatcactat aaccgaaaca ttatctttac cagaaacatc attggccata cttatcaatt
     6841 cttcacttat attactagtt ctctcatatt cataatctaa tattaaatct cttatatctt
     6901 catcatcaat aaacccagtt aatccatctg tcgcaagaag tattatatca ctttttttga
     6961 tttctttctt aaaaatatct acaacaacca tatcatctgt tccaattgct ctagttattc
     7021 tattcctctg tggatgtctt cttgcctctt cttctgtaat gacatgggcc atcataagtt
     7081 cttcaaccac ggaatggtct atagttattt tatcaaattt ttcatctgtt aacaaataac
     7141 atctgctatc tccaacattt gccacatata agacattatt atacactata gccgtaacta
     7201 atgtagttcc cattccttca aattctatat cttccataga ttttttgtgc acaattgagt
     7261 ttacattatt ataagcctgt tttaatatat catctatata gtctattttt acattatcat
     7321 gttgtaataa gttttccttt agaaaatcaa ttatattttc tactgctaat ttactggcga
     7381 cttcaccttt tttatgtcca cccattccat cagctatggc aaatattcct attggaccat
     7441 gttctgtatc tataacttct ccctcacaat agtcttcatt gttttttctt atttttccta
     7501 tatgggaggc acaactataa accatacgac ttcctcctta caaattactt gtattttctt
     7561 ctcaattgcc cacaagctcc actaatatca gaacccatag acatcctaac tgtggcaggt
     7621 atattgttct tctccaaact atccctaaat ttataaataa atgctttatc tggcttttca
     7681 tattctcttt cttcaacttt atttattgga attaaattta catgacatag catacctttt
     7741 aggagcttag ctaatgcctt tgcttctttt tctgagtcat ttaccccttt tataagagaa
     7801 tattcaaatg taacccttct atttgtcttt tttatataat atctacatgc attcaatatt
     7861 tcttcaatag aataagcatt tgctacaggc ataatttttc ttcgttcttc atcatatggt
     7921 gaatgaagtg ataaagctaa atttatagct atttctaagt ctgccaactc atacatttta
     7981 ggtactattc cacaagttga aagagttata tgtctatacc ctatattaag accatttttt
     8041 tcgttaatta tttttaaaaa ttgttttgta ttttcaaagt tatcaagtgg ctcaccactc
     8101 cccatcaata caagatttga taccctctta ccagtatctt cttgaatttt aataacttgg
     8161 tctaaaattt cccatggctc taagtttcta attaatccat ccattgtaga tgcacaaaaa
     8221 ttacatccca tcctacaacc tacttggtta gaaacacaga cagtaactct actatcataa
     8281 tccatcatca cagtttctat tatatttcca tcatctaata aaaacaaata tttttttgta
     8341 ttatctacct tagattctaa ctttaattct atatctatat gccctataca tgaaacctct
     8401 tctaatttat ttctcaaact ttttggtata ttgttcatat catcaaaagt ttttgcaccc
     8461 ttatatatcc aagagaatat ttgacttcct ctaaagggct tttcatctat agttttcata
     8521 aattctttaa gttcatcttc agtaaaattt tttaatacta tctttttctt ttccattctc
     8581 tcactacctt attctcttta attttgctat gaaaaaacca tctataccat gaatattagg
     8641 atatatcttt aaatatcctt tatcttgatt gtctaagtct acatttactg tatcaattgg
     8701 aacaaattca aagttgtgat tttcttcaac aaaagaagta actatattta tattctccac
     8761 accttgaatt gtacatgtac tatatactaa agttccacca actttgacat atttagatgc
     8821 attttctaaa attttctttt gtatagaagt gatgtccttt aactcttctt ctttcttata
     8881 cttaatttct ggtttacgtc ttattatacc aaaaccagag caaggaacat ctgctaaaac
     8941 ataatcaaat ttacttatac tattttcatc aatctcagaa gcatcaaatc tttctacaaa
     9001 cacattttta agccccaatc tatttacagt agagtttata agctttagtt tatgctcaaa
     9061 aatatctcta gctactactt gaccagtatt gttcattaaa gtagctaagt gagtagattt
     9121 ccctccagga gcactacata catccaaaat aaatgaacct tcttttggat taattacttt
     9181 ccctactatc atagaactta tatcttgaat tgtaaataaa ccagccttaa ataattcatt
     9241 tttttctata tttttcattt tttcgacttt aatagcttct tctaccatag gaacttttaa
     9301 acatacaata cccatttcac ttaatttttc aataagctct tctctactta ttttaagtgt
     9361 attcgttctt atatagatac taggcttttc attatttgct tccaacaaat cttcagcaaa
     9421 ttcttgtcca aatttatcta tccagttttt tataatccaa gagttatagg aatactttat
     9481 agataagtat tttacactat cttcctcagt tatgtccatt atagtctctt tagctcttat
     9541 ttcatttcta agaatagcat ttatgaaacc agaagatttt ttatcatatt tcttagataa
     9601 ttttacagtt tcattaactg ctgcataatc tgatatacta tctaaaaata aaatctgata
     9661 tgttcccatt cttaaaaaga ttttaacata agttgacatt ttttttacct ttatttttga
     9721 aagtttattg attatataat ctaaataata tttattttct attactccat aaattaactc
     9781 tgttgcaagt cctctatcca tatcacttat ttctaaattt ttaaaatgct tatttattgc
     9841 aatatttgaa taattgtcat ttttttctat atcacataat actttaaatc caacttctct
     9901 tgcatccata taaatttctc ccttttatta aacataaaaa agggttgttt ctaaacaacc
     9961 cactttactt ttagtcattt cttctttgag ctattagtaa caatctaagt aattgaagta
    10021 ttgaaaccaa tgctgctgca acatatgtta aagctgctgc tgaaagtact cttctacttt
    10081 gtcttgactc actttcatct ataataccta aattcccaag ttgaacgatt gctctacttg
    10141 aagcattaaa ttctacaggc aatgttatta tttggaataa cactgaagcc gaaaacaata
    10201 gtataccaat ttttaaaaat ggaccagcca taataaatcc taaaaatatt aaaaaccaag
    10261 atatacttga agcaaaattc actactggta ctaaactact tcttatttga agaggtgcat
    10321 aacctttagc atgttgtatg gcatgaccac actcatgtgc agctactgca acagaagtta
    10381 tagatgttcc ataatataca tcttctgaca accttacagc ctttcttctt gggtcataat
    10441 ggtcacttag atgccctcta accatttcta tctttacatc atacaaacca tttgaatcaa
    10501 gtactcttct tgctgtttgc tctccagtat atcctcgacg tgtatttact cttaaatact
    10561 tatttgttgt tgaactcact ttaaattgtg catatatagt aagtaatata gctggaatta
    10621 atacaaccat tgttgggtca aatccccaaa aaccgccata tacagggtac ataaataaca
    10681 ctctcctaaa atatattttt attatacatc ctcttaatat tcacttaact taaaaacagt
    10741 ttaattacaa actaatatat tttttcctgt taaaaagcct atagcttttt caatatccat
    10801 catgtctaca ttagtattta cacaaggtcc attaggtctt ttgttaaata cacctaaaac
    10861 aggaattttt ttaatatctt gtattccaga tgtcaaatct ctttcacatg caacagctac
    10921 aacagcttta ggtttagtgt ccattataat ttttctagct agggttcctc ctgtagctac
    10981 aaatattttt acattagttt tttcctttag cttaattaat tctcctatgt tgcatcttcc
    11041 acattcttta caattatcta tatcattagt aacttttaat ttacaactat tctcttgaat
    11101 acaatgaggt attaaaatta ttatatcctc tggattaaaa ttatatttat tactgtatat
    11161 atattcatta tttaacttta tatatattct tctaatatca ctttttgaca taccaaatga
    11221 tgatgcaatg aaagatataa taggaaataa tccactgaca atattaaaat ttattttcat
    11281 tatatttgag ttaacatact ttcctttaat tactctataa gtaactgctg ttgaacatag
    11341 tattataagt attagtgata ttattataat atttatcatc aaggagaata tttgagttaa
    11401 acttattata aaaaaattaa caactcctat caaaattcct aatattaaaa tcaaaatccc
    11461 aacacttgtt aaatacttct ttatatctgt catacttttc acctaaccca atactaaacc
    11521 agtttctata gtattccctt ttatatattc accaaccaac attctctttt tattcggcat
    11581 ctgaatttct tcaattaaaa gtacattatc tatagtactt acttttatcc catctttgct
    11641 tacatcaatt attgtaccta catcttttgt gcttttttca tctaacactt tagttttcca
    11701 aactttcatt atgacatcat tataggtagt ataagcactt ggccatggat ttactcctct
    11761 aactaaattg tgtatttctc tggcactttt tgaaaaatct atgttaccta atgatttatt
    11821 cattattggc gcataggaaa attcttcatg attttgtact tctcttggag catttccttc
    11881 ttcaattaat cttaatgttt cttttaaagt ttctgctcct atattcatca ttttatcatg
    11941 aagttctcct gctgttatat tttcatcaag atttacttct gtctttagta tcatatctcc
    12001 agtatctaat ccttcatcca tatacatagt tgtaacacca gtcttttctt caccatttat
    12061 tattacccaa tttataggtg ccgcacctct atattttgga agtaaagaaa catgaacatt
    12121 tatacatcca agtttaggaa tctctaatat ttcttttgga agtatctgtc caaaagctac
    12181 aactactatt acatctggat ttaaagattt aattttatct ataaattctt tatctctagc
    12241 ctttactggt tgatatacag gtatattatt ttcaattgcc agttctttta ctgggctcat
    12301 acctagtttt ttacctcttc cttttggttt atctggttga gttacaacac ccaatatttc
    12361 atacttctca tctattattt tttgtaaaca tggaactgct atatcaggag ttcccataaa
    12421 tactattttc atcaaatcaa ctcctactac ttttctattt tgtcaacaaa taaaattcca
    12481 tctaagtggt ctatctcatg acaaaatgct ctagctaaaa gctcttctcc ctctaactct
    12541 attgtttcac catttctatt taaagctctg acctttacat aatttggtct tctaacattt
    12601 cccgcttctc caacaacact taaacatcct tcctcatcta tttgctcccc agatgtttct
    12661 attatttcag gatttataag ttctataaga tcttctccaa tatctattac tactactctt
    12721 tttaatattc caacttgagg tgcagccagt ccaacaccat ctgcatcata catagtttct
    12781 gccatatcat ctaataattg tattatcttt tcatcaattt tttcaacctt ttttgatttt
    12841 tttcttaata ctggttctcc tatttgaact atttgtctta aagccattat aattcctcct
    12901 aaatttataa tacactgttt ggattaatat ctatagatac attgatattc ttatcaaata
    12961 tcaaatctcg ttttgttata catatatatt ttattatacc ctttaagaga ttaatttcaa
    13021 tattttcatc tttaaagagt atttgccatc tataattttg atttattttt gatattgaac
    13081 atggatttgg acctaatata aatcctaaat catttatacc ccttttttct aataaataaa
    13141 tcaatgatgc atgcatattt tttatatttt ttatcactaa tctttcatct tcaccactaa
    13201 caactacact taacatatta ttaaaaggag aatatccaaa tgcttttctt atttttatct
    13261 catcttcata aaaaccctca taatcatatt caatagcatg cttaatagca tagtgttcag
    13321 tgtcataagt ctgaagtact acttttccct ctttatcagc tctccctgct cttccagaga
    13381 cttgagttat caattgaaat gtagtttcaa aacttttaaa gtctggaaaa tttaatatca
    13441 tgtctgcaga caaaatacct actaaagtta cattctcaaa atccagccct ttacttaaca
    13501 tctgtgttcc aataagtaca tctgcttctt tatctttaaa tttatttaat atctcatcta
    13561 aagcaccttt ttttgaagtt gtatctttat ccattctcaa agtttttata tctggaaaaa
    13621 tatattttaa ttcctcttca attttttgag ttccaactcc aaatggctta acataagtac
    13681 ttttacattc aggacactct tttggaattt ctttctcata tccacaatag tggcatcttc
    13741 cagtattact tttcttatga taagttaaag atatatcaca attttcacat tgaaacacat
    13801 atccacactt tctacaagat acaaaatttg cataacctct tctgtttaaa aatagtatta
    13861 cttgattatt ttcttcaatc gcataacgta tttctttttg taatttcaaa ctaaatatac
    13921 ttctatttcc tttatctaac tcatctttca tgtctaccac ttcaatgttt ggaagaggct
    13981 tatcatttgc tctacttttt atatttataa gtttgtattc tccagatttt gccctataat
    14041 actcttcaat tgagggcgtt gctgaaccta aaataagagt tatattattt tttaatgcca
    14101 taaatctagc aacttctata gcattaaatt ttggattctt ctcagactta tatgatgact
    14161 catgacattc atcaattatt attaagccta aggaattaaa aggtgcaaat aaagctgacc
    14221 tagctccaat taacactctt actttacctg ctttgacagc tttgtaaaca tcatgttttt
    14281 ctccttcaga tagtttactg tggaacactc caactaaatc tccaaaccta ctctttagcc
    14341 tatctattgt ttgaggagtt aatgctatct caggtactaa gaatatactg tctaagcctt
    14401 gatttagagc aaattctatt atttccatgt aaacttcagt ttttccactt ccagttactc
    14461 catgaattaa gtatggtttt ttatcatcta caaacatatt cgactttatc tcatcaactg
    14521 cttcttgttg ctcattgttt aattttatac tttttagtac ggatttgtaa atactttttg
    14581 gctctctata ataatcctta aattctaggg ttattagctt tttcttactt agagaattta
    14641 tactttgttt tgaagcattt aataaatcca ataaatcatt tatctctaca ttttcattat
    14701 ttttaagaaa acttaatatc tctttttgtt tacttcccac atttatttta ttttgctcta
    14761 tataatcgtc aatcttatca ctttctaaac tcaaagatat ataacatact ttcttttcat
    14821 ttttgtgatt tttatattcc caacataatt ttataattcc atttttattc attgtgtaca
    14881 aagaactatt taagtttggt atatatttta acttatcaac cttaatttta cctttactat
    14941 caatcacttt ctctactatt tctctatttt tatcacttaa agttgatatt ttttccttca
    15001 attcaatatc atttaatcca cttaaacttt cacctaatag gactacttta taattattta
    15061 atttgtatcc ctttggatat atcaaattta tacattctat ataagtacac aaatatctat
    15121 ttttcatcca ataaaccaac tctaaatctt cttttctaaa tattggattt tcatctaata
    15181 tatcaataat ttcttttatt tttatttttt catctaagct atctgtaaat tgaaatacaa
    15241 aggcttctgt tggtttatta ccttttccaa atggtactaa tattctgtgc ccgattttta
    15301 ttacatcaga taaaaataca ggaatttgat atgtaaataa attatctgta tatatagtat
    15361 tacttctaac tattaccttt gcatattttt tcattataaa acctctactt aaatttaata
    15421 tattgttcat attttaatat actttacaag atattaaaat ttcagaattt ataaacaatt
    15481 tcaagttatt aatattaact ttattttaat aattaactat taattatatt ttatacttta
    15541 tgacaaaata aatgctgaaa actacaattt aactgacatt tgcgagtttt tactcgctaa
    15601 aattaaattg cagctttcag ccactttagc tgtgataaaa tacacaaata tcatatttaa
    15661 aaaaggtctt atttctcaag caacgcttta actttatcta aaataacatt ggctacttct
    15721 tcttttttca tcttaggata ctcagtaata tttccctcta catctattat ttttacaata
    15781 ttagtatcag ctctaaaacc agcaccttct ttagttaaat catttgccac aataaaatct
    15841 aaatttttct tttttatttt tcctttggca tgttctataa ggtcattagt ttcagctgca
    15901 aatccaatta gtatcttatc ttcttttatt tttcctattt cataagcaat gtcataattt
    15961 ctacttagtt ctattgataa atcatcattc ttctttttaa tttttttgtc agaataagtt
    16021 tttggtttat aatctgcaac tgctgcactt tgaattacta ttttattttc acttaaattt
    16081 gatactactg cttcatacaa atcttgtgct gactctattt gaacaaattt tttaagattt
    16141 tgtggaatag aaagatttgt aggacctgat attaatgtta catcagcacc tctagctata
    16201 gcttctcttg ctatagaata tcccatttta ccagaagacc tattagttaa atatcttact
    16261 gggtctatac tctctacagt tggaccagct gtaactatca tactttgacc ttttaaatct
    16321 tgctctttag taagattagt caaaacttcc tctactatta tatctggatt agctagttta
    16381 cctgttccaa tatctccaca tgcaagcctt ccactttccg gttcaataaa tttatatcca
    16441 aagctcttta aagtttgtat atttttttga acaattggat tttcatacat attagtattc
    16501 atagctggtg ctataagaac ctgtgctttt gttgccataa cagtagttgt caacatatcg
    16561 tcagctatac catttgcaat cttgcctata acatttgcac ttgctggcac tattaagaat
    16621 aaatctgctt tttttgctaa tgatatatgt tcaacttccc aagtttttgg ttcttcaaac
    16681 atatcttcta ctacataatt ttgactcagc gattgaaatg taagtggttt aacaaattct
    16741 gttgctgatt ttgtcattat aacatggata tttgcattta gtttttttaa cttacttaca
    16801 acatcacaag ccttatatgc agctataccg ccacttatgc caattacaac agttttatct
    16861 tttagcatga ccttactcct cttctgaaat ttgttggtgt tgctcagcat gatgtctagc
    16921 ttcttctatc tctatttcct cttctgtcaa taatctatat gttatttttc cacttgcaac
    16981 ttctttagtt gctacacata caggcttttc cttagtttta tttgaaacat atggttcttc
    17041 tccatcaatt aattttcttg ctctctttga aacagtccct actaaatagt atctgttgtc
    17101 tattttttct agtacctcat tgatagatgg ttttaacata attataattc ctccttaaat
    17161 ttctctatta taatattttt atatctgcta actttattct tttcagatga tattatacct
    17221 tctatttctt tagcagatgt ttttacatct tcattaaata taaaataatc ataatcctta
    17281 atcattttaa tttcttcata tgcacagcta aatctcttct ctatttcagc ttctgtttct
    17341 gtacctcttc caacaattct attttttaat tctttaagtg atggtggtag tacaaatata
    17401 aatactccct ctgggcaaac ttcttttatt tgttttgctc cttgcatttc aatctccagt
    17461 aatacatctt gtcctttttc taaacattcc attattgccg cttttggagt tccataaaaa
    17521 ttatcataaa tttgagcata ctccaaaaac tcaccttttt ctatcatctc ttcaaacttt
    17581 tccttactta tgaagaaata atttactcca tcaacttctc cagttctggg ttttcttgta
    17641 gtagctgata cagaaagttt tattgtatca ttttctttaa gtagttcttt gcaaatagta
    17701 ccttttcctg cacctgatgg tcctgataca actagcaata atcctttttt tacaaacatt
    17761 taagcattcc ccttctaata ttgtattctt actctatatt ttgtatttgt tctcttattt
    17821 tttccaattc gctttttact tctactacta aattagttat atttatatca gatgacttcg
    17881 aacctatagt gttagtttct ctattcatct cttgtattaa gaaatctatt tttctaccta
    17941 tagagtcgtc tttaaatata gtatttttaa gttgccctat atgactttta aatctaacta
    18001 tttcttctgt tatactgctt ttatctgcat atattgcaac ttcttgtgct aatctattct
    18061 catctattat acttgggtca tccagtaatt cacttattct caaattcaat ttttctctat
    18121 aatcttctac aacactactt gaatattttt caatctcttc tatatgattt tttaataaat
    18181 cacatctcat ggcaatatct tcagctaatt ttttaccttc ttcacttctc atttctctta
    18241 atttgattaa tgcatcttca actgcttgat tcaacattga ccacaataaa tcttcatctt
    18301 cttccttttc ttctattttc acaatatcag gaaactttgc aacattcata acgctaatat
    18361 catctataat atcaaattta ttttttattt cttttagtat gtctatatac tgagatgcta
    18421 atccctcatc aaaatttaag tttacatctt ctttgcctaa taagtcaaac tttatgtata
    18481 aatctactct tcctcttttt acataatttt ttactaaatt tctaacttta tcctctaaaa
    18541 atgaaatttt tcttggaaga cgtatgttta tatctgaata cttatgattt atcgttttac
    18601 actctactaa aaagtaataa ttatcatcct tgtattctcc tcttccaaaa ccagtcatac
    18661 ttatagccat taattaaacc tccaaaatac cttcacatat ttttactgct ggacctgtca
    18721 tataagagaa atctcctaca tctattttaa ctgttccacc ttgagacgtt acatttacac
    18781 tactttctac catattaagt aaattgcaca ctattgctga tgcagtcata ccagtaccac
    18841 aacctaaagt atatccacaa cctctttccc aagtataaac atgtatatta tacttatctt
    18901 ctacctttac aaaatttaca ttcgtttttt caggaaatat tttattattt tcaatctctt
    18961 ttccatattt acaaacatca tctacatcta attcatctac tataactact gtatgaggaa
    19021 cccccatcaa catagaagta actttaagct ccctatctaa aacctttata ctttcttgca
    19081 caaaacgttc tttatttgta agtacaggaa cgtccttagc tataaaactt ccttttccca
    19141 tattgactct tatagagttt atttctctat tggtaagatt tacttttata tcttttatac
    19201 catctaatgt atctactgta aaactttctt ttttaactat acaattatca taaacaaatt
    19261 tacaaaaaca tctaaggcca tttccacaca tagctgctct tgaaccatca gaattataat
    19321 ataccatttc tacatctgca acttttgaat ctttcacaac taacaatcca tccgctccta
    19381 cagaaaatct tctatggcat actttttttg ctaaagcagt ataatcagat ggatttatct
    19441 ggtcgaatct tccatctatt gctataaaat catttcctat tccatgtagt ttccaaaatt
    19501 tcataagtat cgctccttcg taaattaacg cttaaactat tataacataa aaaaattccc
    19561 ataaacttat aatttatggg aaaagttttg taatgacaac tataatttga ggatgtgagg
    19621 gagagttgcc attacaatta gggtaattat attatgtttt taagctagtt ctttctatgt
    19681 ttatatatta atatttctat atgaatttta tgtatgtttt atttaaactg tctatgaatt
    19741 ttttaattaa acatctggta actagctata atccttctag tagatatcta ttaacataca
    19801 cctattaatt ctttaagtgt ttttaaaaca tagtctaaat cttccttagt aatatctaca
    19861 tgagttacaa atctatattc accattttcc attttattaa tttttatatt tctttcaaaa
    19921 aattctttta ttaaaacatc ctcttttata atatcttcac tcatcttaaa aaataccata
    19981 cttatatctg gattaacatt atttacttca atgccatcaa ttttatttaa ttcacaagct
    20041 aaatattttg cattttcatg gtcttcattc aatcttaaag tcattttttc aatggcaatt
    20101 ataccagctg ctgcaactat acctacttgt ctcattcccc cacccataac tttttgcttt
    20161 cttctagctt ttgctataaa ttctttactt ccagcaagta tagaacccat tggtgcagct
    20221 aaaccttttg acacacagaa cataacacta tcacaacact ttgctatttc tttagcttct
    20281 acacccaaat ttaatgctgc gttaaataat cttgctccat ctaaatgtac tggtaagtta
    20341 tgtttctcag ctattttttt aatttctctt aaattatcaa gactcacaac tgttccacct
    20401 gaatatgcat tttctacaca tataagccct gtctgtggtt catgtatgtc atctcttcta
    20461 attgcctttt caacaagttc aggatttaga gctccacttt cgtcttcatc tatagttcta
    20521 agctgaactc cagctattac agcaggtgcc ccaacctcat gagtcacaat gtgataacct
    20581 ttaccaataa ttatttcttg ccctcttagg caatgagtaa atagagctaa ttgattccca
    20641 aaagttccac ttggaaggaa aatagcatct tcttttccaa ctttagctgc tgctaattct
    20701 tcaagtttat ttacagtagg gtcatctcta aagattgaat ctccaacagg agcctttgcc
    20761 atagcttctc tcatttcgtc agtaggcata gtaacagtat cacttcttaa atcgataaat
    20821 ctcatttttt caaccttctt tcatatttat atactataga accttaaaat tgattttttc
    20881 tgtaaagttc atatactttc atagcttata ctctacacaa taattatact atatttactt
    20941 tatatttaat ctactttaaa ttattaattt taaaaataaa gtttcctctc taataaatat
    21001 aaataatcat acaatttaaa tattaatctc tattgtttaa attcccccta gtcacaaagc
    21061 gtatattatg tgtataatta atttactgac tcattttggt caaagaaagg ggtgctataa
    21121 atggcattag atggattagt tatacattca atagtcgatg aattatcatc aaaacttaca
    21181 ggtggaaaaa tagataagat acatcaacct gaagatgatg aggtaatttt taatataaga
    21241 aataataaag aaaattttag attggtttta agtgcaagtg cctcaaatcc tagagtttat
    21301 cttacaagta attatcaaaa agaaaaccct ctaaaagcgc ctatgttttg tatgttactt
    21361 agaaaataca tacaaggtgg aaatatagta gaaatttctc aaataggttt tgaaagaata
    21421 ataaaaataa gtgttgaatc tttagatgaa ttaaaagaaa agacagttaa aaatattatg
    21481 attgaaatta tgggaagaca tagcaacata ataataactc atggtgaaga aaataaaatt
    21541 atagattcta taaaaagagt cccttttagc ataagtagag ttcgtcaagt tttacctggt
    21601 catgattatt cattaccacc agaacaaaat aaactcaacc ctcttgatga tatatctaag
    21661 gatttgttta ttaaaaacct agaggaattg gaaggaccta tctttaagtc tatatattct
    21721 aaatttctag gtataagccc tattatagct aaagaaattt gctacagagc aggtgtaaat
    21781 caaaatgcta tcataaagga tatatctgat gaacaatttg attctttaca caaagtattt
    21841 tgtaatttat tcaatgatat aaattctaac aaatactctc cttgcattat tatagataaa
    21901 aaggtggata aggtagttga tttcagttgt ataaacctta cattatttag cgatttatca
    21961 tatataaata aggatagtat gtctcgtatt ctggaggatt tctatagaac taaggatatt
    22021 aaagatagaa ttaatcaacg ttcatctgat ttaaagaaga gtatatctgt taagcttgat
    22081 agactttata ataaacttaa aaaacaagaa gaagaattat cagaatccga aaatgcagac
    22141 atttataaga taaaaggaga attgattact tcatatattt atatggtaga aaaaggtatg
    22201 gaaagtattg aagttgctaa cttctatgat gaaaattgta atgatgtaac aatagaatta
    22261 aataaaaact tgacaccatc tgaaaatgct caaaaatact ttaaaaagta taacaaaatg
    22321 aaacatgcta aagtcgagat atctcatcaa atttccttaa ataaggaaga aatagattac
    22381 ttagaaaaca taattttaag tattgaaaac tgtgaaaatt tagctgaatt acaagatata
    22441 aaagaagaac ttgctaaagt tggatacatc aagactcaaa agaaaaacag taaaaaggat
    22501 actatcccat ctacaaaacc tcatgaattt gtttcttctg atggatttaa aatattggtt
    22561 ggtaaaaaca ataagcaaaa tgactatctt acactaagac ttgctgataa tgatgactta
    22621 tggatgcata caaagaatat tcctggttct catgtaataa taaagtgtgc tggtaaggaa
    22681 gtccctgata atactgtatt tgaaggagct atgctagcag cattctttag taagtcaaaa
    22741 ctttcttctc aggtgcctgt agactatact aagagaaaaa atgtgaaaaa acctagtggt
    22801 tctaaaccag gtatggttat ttatgaaact aacagtacta tttatgttac tcctgaagaa
    22861 gaaacagtag ccaagcttaa ggttaaatct gaataaattc tgcactaaat acaaaataaa
    22921 tgttagacaa ctacacatta tcattctaaa taaaaatagt ggccttctta taaagtacaa
    22981 taataaggcc actttctaat tagcttactt ttaattttct aacaaaatac ttttctgttt
    23041 taataactat acttatattc ccaacaaata ttttcagtct tcttatatag atgtaattaa
    23101 aaagttaaca ctaaaagcta ttttttaatt tattgatttg ttttagtaat tcattatatt
    23161 ctgattcata caattctttt tttagattat ctttttctat agataacata gatataacag
    23221 ttgtaagtct attttctaat actagaagat tttctttttt gattcggttt tctctactag
    23281 gagttttttt atttctgcta attgtatatt cgtcataatt acaatcaaac tcccttattt
    23341 tagcatcttt tatctctata atatagttac aaatatgtga tacaaatact ctatcatgag
    23401 acactataag catagtttta tcagtattta tcagtgcctt ttccagtgct tccatagatt
    23461 ttatatctaa gtaatttgtt ggttcatcaa atactaaaaa attattatct tccaatatta
    23521 ttttacatag tgctattttt accttttctc caccactaag tacttttact tttttgtaga
    23581 catcatcacc tttaaaccca aataggctta agtttatcct tataaaagat tcatcaaatg
    23641 aactattaac tttagtattg tatagaacac tcttttcctc atctaataaa ctttgatttt
    23701 ggtcaaaata acctactttt accttgttat ttatttttat attgtcattt ttatcagcca
    23761 atatttcttt tatcagagta ctttttccac acccattatc acctaacaat gctatctttt
    23821 tacctctttt tatcttaaag gaaacattat ctaacaggag cttattttct aactttagag
    23881 tcatatcttt tacctctact agatttttgc taattatctc cataccatct tgcatcttaa
    23941 tgttcatttc tttagaaact ttaggtttat tctttacctc aagtttatca attctgcttt
    24001 ttatagcctt cacatttgag tccaactttt tcttattagt ctgaccaccc attttgtgca
    24061 acctagcttc tgaattaccc attctttttg gtgtttttct tatgccatca cttaaagctt
    24121 ttttatataa aagagctttt tcaagccttt ttttctcttt aacatattct tgatactcaa
    24181 aatcagctct ttttacttct tcatcttttt gcattaaata atcagtgtaa ttgcctttgt
    24241 aagcttttag ttttccatct tctagttcta atatagttgt acataactca tccagaaaac
    24301 gtctatcatg agatattaat aataatgctc cctcatgcct tttaaacata tcttccaaaa
    24361 ccccaatgct ttttttatct aagtttgaag ttggctcatc agctattatg atatttttct
    24421 tattactcag cgcatctgct atttttaact taaccttttc ccctcctgat aagtgttctt
    24481 caaatttatc tggagcattt aaaagactct tctccttact atgactacat gtttcaatat
    24541 tattttcact ctgacttata taagaatagc tttctgtaag gtaagtatat ccttcatcaa
    24601 tttctatttc tcccaatatt gcttttaata atgttgtttt tccttgacca tttgaaccaa
    24661 ctagacctat tttgtcattt tctaatattt caagtttatc tatatccaaa attaatttat
    24721 ctgcataata tttctttaaa ttctctactt ttactaacaa cataaaaaat ccctccttaa
    24781 cacacttaaa tataaggaga gattctataa ataggcataa caaataaacg tatagactat
    24841 aatctactct ccttaattta agcattgcaa ataaagccac caaaataggc ttttcaaaaa
    24901 ttacaatact ttaaattata aaaaatagat tatattctca ttgtcctagt taccttgccc
    24961 tttctttcat ttatttttaa tcattatatc aacattaatt acaaatgtct atagaaaatt
    25021 aactaatttt agttattcaa tatacaactt attctttaaa tttattcttc ttcaacttta
    25081 aagtcctgat tatttaaaat tctgtttaat acaattccaa caattgcagc taaactaaga
    25141 ccagttatct ttactgtact tgttataggg attccaatag aaataccttt attagataaa
    25201 tatgttgttc caagacctat taataatata actgctatga ttaatatatt tattttactt
    25261 tctacacaag aactgtccct tatagttttt aatcctacat aagttatcat cgaaaatagc
    25321 attatactaa ttccacccat aactgaacct ggtatacttt gaagaaatcc accaaacttt
    25381 cctacacatg caagcaatat agcaaatatt gctgtacgtc taagtattga agggtcatag
    25441 tttttagtta tagcaagtac tgctgtattt tctccataag ttgtatttgc tggtcctcct
    25501 aaacaccctg caactatagt cgcaaatcca tccccaagta atgttctatt taatccggga
    25561 ttttctataa agtttttacc tactacagca ccattagtag tcatatctcc tatatgttcc
    25621 ataaatacag ctaatacaac tggggatata atcacaattg ctcctaaact gaacttagga
    25681 actgtaaagt taggtattgc aaataaactt gcttctgtaa tagtagttat atctactaat
    25741 cttaatatca aacagattat atacccagaa aaaactgcta ttaatattcc taattgtttt
    25801 ataaatcctc taccaaactt attggttaaa attgctattg ctaaagttat aaatgctatt
    25861 acaaaattgg ctgaagccat actaaaagct gttggcaata aatttaatcc tataactact
    25921 atcatagctc ctgtaacttg tgcaggaaaa tataatttaa ttttatttac accaactact
    25981 tttactataa tcgacattat aatataaatt aatcctgcta ctattatacc accttgagca
    26041 tatgctaaat ctccaccata tgcctcttta gctgctagta taactggtat aaacgcaaaa
    26101 cttgaaccta aaaaggcagg tacttttcct tctgtacaaa aatgaaatat caatgtacca
    26161 acaccagcac agaaaattgc aactgacgga ttaaaacctg tcaaaatggg aactaatact
    26221 gtcgctccaa acatggctaa tagatgttgt aaagacatta tcgttttttt cataaaaacc
    26281 tccaaaatta ttatatataa ataaatactc tgtttaatta atatctttta attaattatc
    26341 attccctaga ttctattttt taacctctat atacttatta tataatttat cattatttta
    26401 cattttacga catcaaaaac tcaaataaag gagaataaaa actacattaa gactcacatc
    26461 cataaaatta taatatttat tttgtgcaat ctatgtaagc tttatcctcc tccctgacta
    26521 ttcaatgcta agcttttata taatacttaa aaataattat tctttgattg taactgaatc
    26581 ttctccatca atttctaata aactgacaga tataatttca tgtctagaag taggtacatt
    26641 ttttcctaca taatctgctc ttataggcaa ctctctatgc cctctgtcca ctagcactgc
    26701 taattgtatt gatttaggtc ttcctatatc tataatagca tctagtgaag ctctcacagt
    26761 tcttccagtg tataaaacat catcaactaa aactactgtc ttatcattta cattaaaatc
    26821 taaataagtt cctttaacta caggttcaac atgaattttt tccaaatcat ctctatacaa
    26881 agttatatca atatctcctg tatctacttt tgttccttct atttgttcta ttttcttaga
    26941 aattctattt gcaattggca cacctctagt ctttattcca actaaaacta gattttctac
    27001 acctttattt ctttcaatta tttcatgact tattctagta attgctcttg ctattgcctt
    27061 ttcatccatt aattgagctt tttctaccat atcataatta ttgtcaaaga tataatatat
    27121 ctatttgaca aatctactcc tttcttttaa ttttttataa atttattttt atttaaaatt
    27181 tcaatttaaa caacaaaaaa acttctcatc acaagacaag aagctcttat ataaaaaata
    27241 tacagtgcaa tttagcaatt gtcttacgat atctctgtat cgtttaaaga taatttttta
    27301 atctatcaaa tcaaattata tatttatatt tcaaattttt caagcttttt taaacaaaat
    27361 actttaatat aagatatttt atttaaattt cactaaattt atttacttat ataaaaacaa
    27421 aaaatgattt ataagtatta tacaaatggt tattttatct ttttaataac ttccttaaaa
    27481 tactcaggta attctgaatt aaattcgata tattccttag tagtaggatg tataaatccc
    27541 aacttcttag cgtgcaaagt ttgtcctttt aatttaaatt tagtttcttt tggaccatat
    27601 acataatcac ctaaaagtgg gtgatttata aacaaagcat gaactcttat ttgatgagtt
    27661 ctaccagttt caagcctagc tctcatatgt gtaaacttat caaatctttc tacaacttca
    27721 aagtgagtga ttgcattttt tccatctttt actacagcca tttttaatct atccttagga
    27781 tttctcccta taggtttatc aacagttatt ttatcttctt taaccacacc atagcatata
    27841 aattcatact ccctagttat agtatgttcc tttaactgtt ctgctagaaa attatgtgca
    27901 taattgttct tagctatcat aagaagacca gaagtatctt tgtctattct atgaactata
    27961 ccaggtctaa taactccatt tattgatgac aacttatctt tacaatgata taaaatcccg
    28021 tttactaacg tattttcata gtttccagga gcaggatgga caaccatatc ctgaggttta
    28081 tttattatta agacatcatc atcttcatat acaatatcta ttggaatatc ttgaggtaaa
    28141 acttctaata ctttaggttc tggtatctct ataacaatat tatcttcctc tttaaccaga
    28201 tatctagctt tttctacttt attattaacc tttacttttc catcttttat aattttttgt
    28261 atataacttc ttgacatatc acctaattgt tctgctagat aaatatcaag tctaacttct
    28321 tcttcctctt caagtactaa aaactctttt acttcatcca ttatatcacc tacttttact
    28381 ttcaaaaaat aaaacatata tacataaaaa cacagttcct acaactacaa atacatcagc
    28441 tatattgaat acatattccc atataattct aaagtcgaag taatctacta caaaacctaa
    28501 tcgtactcta tcaattagat ttcccaatgc accagatata attaatataa ttcccaacct
    28561 tccaaatata tgtactttcc ttgtattaag atagtatagt ccaaatactg ttgcaagtaa
    28621 tgcaacaatt ataaatatcc attgattatt ttgtaataat ccaaatgctg cacctctatt
    28681 ttcgacataa gttaaatgaa atacattatt tattattggt attgttgata catccaccaa
    28741 attattcaat acccatattt tagacagttg gtctaaacct atgagtagaa ttattattaa
    28801 tatatatagc aatgattttt ccccctataa attatttaaa aaccaatttt atttgaagca
    28861 cactattatt attatacaaa aaaaatgctc taaagagcaa ctttattaaa attgtatttt
    28921 cttaataaaa ctagaacaaa atttaaaata gtactacttt ttttatattg tagatttcta
    28981 catatttaaa atcatatcat tatgtataaa aaatttaaaa ataccatact aatcaatatt
    29041 ttttgtgtat tttatagatt aaagattaat gatgtctgta atttaaactt acaatcacca
    29101 ctaatcttca aacaaatatt tttacttttg cacttgctta tttttatcta aatgttgtat
    29161 aagttcagat actgttacga atttatatcc tctctctttt aatttaggaa ttactacttc
    29221 taaagcttgt atagtttgtg atttatcatt tcttttagtg ttataatcat gtaaaagtat
    29281 tatattacca ttttggacct ttgtaaggat agtatttata attgcatcta ctccagggtt
    29341 agaccaatct ctagggtcta agtttgacca aagaattata ttcatgcctt tagaaactat
    29401 aatatcacag acacttttac tcatggctct atatggaggt ctaaatatct ttggttctac
    29461 tcctattaca ctttttataa tattttgcgt atctgttatt tctttttcaa cactactata
    29521 actatttttt gcaacattta tatgtgtaaa tgtatgattc cctatttcat gcccctcatc
    29581 atattgtctt tttaataatt ctttgttata ttctactttt tctccaacta caaaaaaagt
    29641 tgcttttaca tcatttttct ttaatatatc aagtacttgt ggtgtaaaat cctcatctgg
    29701 accatcatca aaagtcaatg ctaaaatttt ttcatcctca tttccttttt ttattattac
    29761 atcttcataa tcgtctttta tattgtcatt taaactatat gcttcttttg aactatcttg
    29821 attatgtgta ttatgattat atgtatatac acctactata gataataaac atatacatat
    29881 accccatatt attttcttca tgtcattacc tcttttctaa ttcattctag caatatattt
    29941 tataatgtca gttttttaat ctctcaatat tccattttta tttaaatcag ttaaatttgt
    30001 taggtcttct gaataaaagt tagaatcttc tacatcaggg ttttgcattg catcacctaa
    30061 taaattattt tctttctttg cacaatcatt acataaactt gtatctggca atatatccaa
    30121 tctttctaaa tctatttgat tatgacataa atcacaaatc ccatatgttc cattctctat
    30181 tttagataat gctaagtcta tttgatacaa tcttccctct tcatgattta ttagactatt
    30241 ttgcatattt tgcatatata aatctgttcc catatctcct atatgattat cataactaga
    30301 taattctcct gatgaatact tttcacttgt atgtttagtt gtatctccaa ataaagtatt
    30361 atctttcata tcctctacta aattagttaa attttctctt tctttcaata atttatcttt
    30421 atatttttta gtatccattt ttaacacctc taattttaat atttacatca taaaaatagt
    30481 atgtggaaaa gtaaattaaa catacaataa aaataagatt tgattcgaaa aatagaatgt
    30541 ctcaaaatga aaattttagt tcatttaacc ctctttttta tctgaaatca aatatatttt
    30601 tatcctttca acaatgaaaa agagacttat ctctattttg agatagtctc ttctttgaac
    30661 ataatttaaa tataattttt taatttttat agttattatt ttactaactt gccaatttat
    30721 gattattttg ttgcttaata tgctttgctt ttttaaagaa taaaagacca agaattattg
    30781 ctacaataac acctattcca ttagatatat taactggtag tctaaatcct tcttttgctt
    30841 gcattatata actaaatgtt actacagtca taaaaagtgc tggtaatgta gttatataat
    30901 ggtttttatt tgctttgact aaatatgcag aaccagtcca caacattatc attgcaagtg
    30961 tttgatttgc ccaagagaaa tatctccaaa ttatattaaa atctataaat gtaagagcaa
    31021 caccaactgc gaataatggt aaagctatta aaaatctatt tttgactgca tcttgattat
    31081 aattcattgc atctgctatt gtcagcctag cacttctaaa tgctgtatct ccagaagtta
    31141 taggacatgc cacaactcca agtatagcta aagctcctcc aataggaccc attaatccaa
    31201 cagatactgt atttactact gttgctgctc caccatcatt aaatatcaca ttaagttgag
    31261 gtatgccatg gaaatatgac atggctgctg ctgcccaaac taaagctatt ataccttctg
    31321 caaccattga cccataaaat actggtcttg cctcactctc tttctcaaca catctcgcca
    31381 taagtggtga ttgagttgca tgaaaacctg atattgctcc acatgctatt gttataaata
    31441 agtatgggaa taaaggtgtt ccatttggat tcatattttg taaatttata tttggtatgt
    31501 tatacccctt tattattaat cctccagcta cacctactgc cataattaat aatgctgctc
    31561 caaataaagg atatatttta ccaatcaatt tatctattgg taaaacagta gccataatat
    31621 aataaagtat tatcactcca acccatattt gtgcgtttac acctgtcaat gtctttaata
    31681 atcctgctgg acctgttaca aatacaactc caaccaatac taaaagaata acactaaata
    31741 ttctcattgc tatttttgca ccatttccta gataaaggcc taccaactct gatgcagatg
    31801 tacctttgtt tttcattgat agatatcctg aagcaaaatc gtgtactcca ccagcaaaga
    31861 ttgttccaaa cacaatccat aaaaatgctg atggtccaaa taatgctcct tgtatagctc
    31921 caaatatcgg acctaaccct gctatattta gaaactgaat taaaaagatt ctcttccatt
    31981 tcattggcac ataatctact ccatccctac aagctatagc tggagtttga tttttgtcat
    32041 tggttccaaa aaccttttct acaaaagtac catagacaaa ataacctact ataagtacta
    32101 ctattgaacc taaaaagctt accataaatt catcccctta gtttttatat aattgcttta
    32161 taaattttta tgagtttaag aaaactattt ccagttcaaa tagtaatact tttttatgct
    32221 aatataaata ttaattacat taagtgcata tttaagtaca tgaaatgact ttatatatgc
    32281 attaacagcc taaattcttt tactttgctt ctacttacag ttacttcata atcactattt
    32341 tttaatttta atatgtatgt attattaaac catggtataa tttcttttac tttggttaag
    32401 tttacgatat aagacctatg tgttctaaaa aatgttttat catttataag attttcgact
    32461 tctgatatgc cttcttttaa tataaacttt tctttctcag tatatataaa tgtctgcctt
    32521 tcattagctt cacagtaata tatatcatca atatctatta caactaattt atcacctttc
    32581 caaaggctaa ttttatgtgt tatttcttct atctcctgat ttactgcttc cttcttatat
    32641 ttgtataaat tactatttac attagatagt tcaatatcac tcatctcact tttttctaat
    32701 ttattcagca tagaaataat tctctcttca gaatatggtt tcaatatata atcaaataca
    32761 tccaaagaaa aagcatctac tgcataactt tcatatgctg ttataaaaac aattttaggt
    32821 cttgatttaa actgatttaa tgtttttgca agtagcattc catcaagatg aggtatattt
    32881 atatctaaaa aaattacatc tattttattt tcctgaataa agtctaacac ctctatacca
    32941 ttggtaaatt cacttactac ctcgattcca cttttatttt caataaaata tctcaattcc
    33001 tttcttgctg gaaattcatc ttccactatt atagctctca tacttctact cctttacata
    33061 aaatactatc aaagtcccat tatctattct tcttatatta agccctcttc catacatcag
    33121 tttaagcctt aaatgtacat tataaagccc aatcttattt tctggcatat tatcttgata
    33181 tacattattt ataatttcct cacttatacc tataccatta tcctctattg atattctcac
    33241 agtattttct aatgaatacg gtaacttttt tatggatatt ttcactacac cagctctacc
    33301 actttctaaa ataccatgta ttattgcatt ttccactaat ggttgtataa tcaaacttgg
    33361 aatctttata tttacatctt ctatatcata agatacagtt agtaattctc caaacctagc
    33421 tttttctatc tctacaaaag atttaacttg ttcaatctct ttatttatat caattaagtt
    33481 atcactatat tcaagattat atcttagata agttgataaa tttattatta actctctagc
    33541 cttatctgga ctaaatctta taaatgaagc tattgtgttt agggaattaa acaaaaaatg
    33601 tggattaatt tgtctttgaa gtgcttttat ttctgcttta tttctcgctt ctatatcttt
    33661 tttatctttc tcaatcccct ctactataga tataacaaat cctattccaa tctgcccaac
    33721 tattaaaggt aaatatattc caccgattat ttgaattgct aaagaatgtg gatatgatat
    33781 taagtatata agtaatatag taaaactttc agaaatcatg cctaccaata ttccatacat
    33841 tactctatgt tgttttggta tcctcttata tagaaatcct gataatacac ctgctagtat
    33901 acttgataaa agacatggaa cagatgttat ccccccaata tccataaaat atctatgaat
    33961 ccctgaaaaa actcctgctg taatacctac catcggacca aataaaatgc cacctgatac
    34021 tatagatatt attctagtgt ttactattga ccccatataa tttataccac tataagttcc
    34081 aaatatggct aaaaaagtaa aaactaatgc tatacagacc aagtcattaa aactatattc
    34141 ttccttttga aatatctgct taaatatttt taactttgtt attagaagaa acaatactac
    34201 aataaggctt gtcttttcta ttaatgcgat taaaattgat gttttcataa tcccccttta
    34261 aaaaataaat ttttacacac tctaaattta ttatggttgt tataaattac atatttgaag
    34321 taatcctatc acaaaacttt ccacatttaa tcttttaatt aaataagaaa tagttgtact
    34381 tctattaaca actaatacta gctagcataa gcttgtaata acttgtagtt attattaact
    34441 ttacaattct agtatttaat tttatacttt agaaatctct tttcttagaa ataatacttt
    34501 cgaatatcat ataatacaat attatgtaga ttcttactca tacaacatat ttctttaaaa
    34561 attttatact attctttgtt caaatatctc atttgattta attgtgttaa acacacatta
    34621 tcatctctct gttatatatt tattatatag ccgtatatcg ataaagtaaa taaatcccat
    34681 atttaaaata atatgtt
//
LOCUS       DAGSWI010000030        34382 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.030, whole genome shotgun sequence.
ACCESSION   DAGSWI010000030 DAGSWI010000000
VERSION     DAGSWI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.030"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(90..443)
                     /locus_tag="MJF80_001306"
     CDS             complement(90..443)
                     /locus_tag="MJF80_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279978.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(460..5781)
                     /locus_tag="MJF80_001307"
     CDS             complement(460..5781)
                     /locus_tag="MJF80_001307"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279979.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDSKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDVFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNALISQYT
                     EISLSNVTDAYSSYNATFIVVDEKKVYATGINTNYLLGFSTSDGSNVNLGLLSDWYYI
                     NISGSSYSRVSCTNNITKINNIIIYEYVTVFCTNIGSFLTGYHGTSWTKPTDSSYRVQ
                     YQGISYAGYLDSYIYNYYPTRCTQSSSSTTFAYLYNGESSSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNIFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYLCNDVSIIVKNDNTCYVCGLVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(5798..6781)
                     /locus_tag="MJF80_001308"
     CDS             complement(5798..6781)
                     /locus_tag="MJF80_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBZ0279980.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(6782..7480)
                     /locus_tag="MJF80_001309"
     CDS             complement(6782..7480)
                     /locus_tag="MJF80_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBZ0279981.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(7477..8529)
                     /locus_tag="MJF80_001310"
     CDS             complement(7477..8529)
                     /locus_tag="MJF80_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBZ0279982.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTVI
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYLRDIRGEIIYTKVMGILINTTGVHDLSKLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(8522..8950)
                     /locus_tag="MJF80_001311"
     CDS             complement(8522..8950)
                     /locus_tag="MJF80_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBZ0279983.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(8950..9276)
                     /locus_tag="MJF80_001312"
     CDS             complement(8950..9276)
                     /locus_tag="MJF80_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HBZ0279984.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(9292..10821)
                     /locus_tag="MJF80_001313"
     CDS             complement(9292..10821)
                     /locus_tag="MJF80_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBZ0279985.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGIVTLNVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(11103..11525)
                     /locus_tag="MJF80_001314"
     CDS             complement(11103..11525)
                     /locus_tag="MJF80_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279986.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(11525..13978)
                     /locus_tag="MJF80_001315"
     CDS             complement(11525..13978)
                     /locus_tag="MJF80_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279987.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(13978..14145)
                     /locus_tag="MJF80_001316"
     CDS             complement(13978..14145)
                     /locus_tag="MJF80_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279988.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(14172..14618)
                     /locus_tag="MJF80_001317"
     CDS             complement(14172..14618)
                     /locus_tag="MJF80_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBZ0279989.1"
                     /translation="MSENGLLKNINIVDLLLNADTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(14744..15172)
                     /locus_tag="MJF80_001318"
     CDS             complement(14744..15172)
                     /locus_tag="MJF80_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBZ0279990.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(15186..16250)
                     /locus_tag="MJF80_001319"
     CDS             complement(15186..16250)
                     /locus_tag="MJF80_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBZ0279991.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(16255..16698)
                     /locus_tag="MJF80_001320"
     CDS             complement(16255..16698)
                     /locus_tag="MJF80_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279992.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(16947..17387)
                     /locus_tag="MJF80_001321"
     CDS             complement(16947..17387)
                     /locus_tag="MJF80_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279993.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(17492..17689)
                     /locus_tag="MJF80_001322"
     CDS             complement(17492..17689)
                     /locus_tag="MJF80_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0279994.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQS"
     gene            17858..18178
                     /locus_tag="MJF80_001323"
     CDS             17858..18178
                     /locus_tag="MJF80_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBZ0279995.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            18260..18766
                     /locus_tag="MJF80_001324"
     CDS             18260..18766
                     /locus_tag="MJF80_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0279996.1"
                     /translation="MNNLDKLFELASQEGIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            19467..20222
                     /locus_tag="MJF80_001325"
     CDS             19467..20222
                     /locus_tag="MJF80_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBZ0279997.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(20365..20793)
                     /locus_tag="MJF80_001326"
     CDS             complement(20365..20793)
                     /locus_tag="MJF80_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBZ0279998.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(21214..21414)
                     /locus_tag="MJF80_001327"
     CDS             complement(21214..21414)
                     /locus_tag="MJF80_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBZ0279999.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(21597..22766)
                     /locus_tag="MJF80_001328"
     CDS             complement(21597..22766)
                     /locus_tag="MJF80_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBZ0280000.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSEDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            23002..23823
                     /locus_tag="MJF80_001329"
     CDS             23002..23823
                     /locus_tag="MJF80_001329"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBZ0280001.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIHIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(23899..25287)
                     /locus_tag="MJF80_001330"
     CDS             complement(23899..25287)
                     /locus_tag="MJF80_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HBZ0280002.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFDKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVRNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(25422..26180)
                     /locus_tag="MJF80_001331"
     CDS             complement(25422..26180)
                     /locus_tag="MJF80_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBZ0280003.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSAIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(26182..26898)
                     /locus_tag="MJF80_001332"
     CDS             complement(26182..26898)
                     /locus_tag="MJF80_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBZ0280004.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLICSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYIFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(26900..27607)
                     /locus_tag="MJF80_001333"
     CDS             complement(26900..27607)
                     /locus_tag="MJF80_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBZ0280005.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            complement(27723..28259)
                     /locus_tag="MJF80_001334"
     CDS             complement(27723..28259)
                     /locus_tag="MJF80_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBZ0280006.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(28361..29431)
                     /locus_tag="MJF80_001335"
     CDS             complement(28361..29431)
                     /locus_tag="MJF80_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBZ0280007.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(29424..30482)
                     /locus_tag="MJF80_001336"
     CDS             complement(29424..30482)
                     /locus_tag="MJF80_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBZ0280008.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(30469..30801)
                     /locus_tag="MJF80_001337"
     CDS             complement(30469..30801)
                     /locus_tag="MJF80_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBZ0280009.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            31000..32121
                     /locus_tag="MJF80_001338"
     CDS             31000..32121
                     /locus_tag="MJF80_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBZ0280010.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(32249..34147)
                     /locus_tag="MJF80_001339"
     CDS             complement(32249..34147)
                     /locus_tag="MJF80_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBZ0280011.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT        11034 a         5910 c         3673 g        13765 t
ORIGIN      
        1 caaaaaataa gtacaaggct tacttattaa agttaagacc ttgtacttta cataaaaaaa
       61 ataactaata catagttatt ctaaatttac tattcaactg gtatttctaa ttctttacat
      121 ctaattctaa cttgctctct aattactgct ggtacttctt ctattgtttt tttgcctctt
      181 aaaattaaag ctacatatac ttctaccatt ccactctcac ctccagattt tcttacacta
      241 aaagtgctta tattacttgc ttcattgaca gaactttgat ttaacaacat ctcatatatt
      301 tctgcatttg ctataaaatt atttgttata tcatcatctt gctttacttg acctatactt
      361 atgtctgaaa tgtctttata ctgcatttct acttttctac cttgtttgat ttgttctatt
      421 ttatttttat cgaatttaaa caattttcac acctccattt taagcttgtg gctcgttgtt
      481 ttcagcatca tatctaaaaa tcatagatgg acgttttata gctgtccatt tatctccttt
      541 ttctactatg attttaggct gagcactttt tatgttggct tcttctaatt catagatttc
      601 ttggtaactt gagtctgatt ttttgatttc aactagtttc atgtctttga ataagtcaga
      661 tttatttatt tttacttgaa ctttagtttt atttattaat tgatatatag gattgttttc
      721 catcatatca tcctttaatc cctcatttaa tgttactatt aagtcagtgt tattttcagt
      781 aatgtttatg attgaatata ctttattgtt tatcattaaa atagaatcct tttttacttt
      841 aagccctgtt tctgatttaa atataaacat acttgaatat aaatcgcctg taggactact
      901 ggctttaatc agtatcttat tttctcctaa tactatttta ttttgtggta tctcaaatat
      961 aatgctattt atttccaaat tagtcttagt agatattaat tcatcattta taaacatttc
     1021 aagtttttct ataacattat tagggtcttg tactacaact ctcacttgag atattgattt
     1081 ttcaactatt tctatagatg ctttacttgt atctactttt gttatcatgt atattgtatc
     1141 atctgaaagc tctatatcta aaatatcatt tagatgttca ggaattggta ttttagtaaa
     1201 ttcatataaa tcagtaattg atatagatat tttatacata gatttatttt taccagttat
     1261 ccatagacta ttatcatttt taattactag tatgtggtca tcattatgtg acttaatttc
     1321 tttaacatta gttaatactt tagtaaaagt tcttattatc ttatcttttt cactttctcc
     1381 tagtccaaat aatccatttg tattgtaccc ttgagcgtac aactctccct cattagttaa
     1441 aacaagtgtc attgagttat atatacttat atctttaaca gaaggtaatc cagtaacctt
     1501 ttgtaactct cttacatcat tattgtggcc aactcccaat tggtactcag tattctttcc
     1561 atatgaccat agagtattat ctgtcttaat aatataggag tagttatctc cacaagcaac
     1621 ttttttgaca tctgatatat ttacttcagt aagagttaat aattctatat cttcaagggt
     1681 ttgattaatt tctttatatg aattacttcc tattccatat aacttgccat ccttagcaac
     1741 agccaatgaa tgtgtttttg atatatcaat atcagatacc tcaaatggca atattattga
     1801 agtatactta aaattatctt taaatatgtt tttataattt atatacttat tatttgtttt
     1861 tatatactta tcattaaaca ttactagctt ttcatttttc ttattaaaaa ctattttatc
     1921 tgatgtgtta tctgttttaa aatatgaatg tagggaacct tcaagagctt catttttatc
     1981 attatcatat ctatggtgaa tacctaaatg attatatcta ttatctcctg ttgaagaata
     2041 aaattttcca tatatatctt taataaatat agtattgtaa ctagaaaata cagtatcaac
     2101 atctttcttt atagatactt tataatatgg aactgacata tccctataag attcatcaga
     2161 tataccataa gtatctctac ctgatgatat caaattgtta ttattatcta tataaagtgt
     2221 attattagct gttacaaaaa catctttaac atcttccaca ctatttgtat tattattatt
     2281 tgctatactt ccatatccac taagacctcc aacaaatagt ttattgtcat ttgttaaaat
     2341 ggctgaagta tatccattaa ctactatttt ttttgcattt ataatattat taattactcg
     2401 tatccccttt atatcattag tctctgttga aaagaatacc ctctcttttt tccctgtagt
     2461 ataaatcata ttgtcatttg ttataaaaaa tgtagcttca cttcctgctg tcacaacaga
     2521 ttttacattc tcaatattta tttttgtaaa tgtacttata cttccttcag taaacccaaa
     2581 atagccattt acaagtccac atacatagca tgtattatca tttttaacaa tgattgatac
     2641 atcattacat agatatacat ctctaacacc atttataggt atacgtgtaa atatattcct
     2701 acttgtagta tcacccaatc ctaactgtcc ttttccatta ttacctgctc catataaaag
     2761 accatcatta gttaataata gagatgtgtt tccatataca gctacatatt ttacattttc
     2821 aacatttatt ttagtaaata tatttctatt tacattatct cctattccta actgaccaga
     2881 attatttaaa ccaacagcat aacaggtatt atcatttttt atcgcaaatg catgtttact
     2941 attgtcagta aatctattta catttataga ttttatatta tcaatattta ttttagtaaa
     3001 tacctttcta tttatctcat ctcccactcc taattgacct acatcattta aaccaacact
     3061 gtaaagcata ttattattag ttaataaata tgtagacttt gaatggctat atatttcttg
     3121 tatattagaa gatatttcac tactgctatt ctttttattg taatttatct tattatttaa
     3181 atttaaacta ctattaactg atataccatg cgtaccctta gagttatatc cacatataaa
     3241 taagctctta tcagttaata ttgcaaatct agttttatct ctacttaaat cataaggagg
     3301 tataaatata ttttttatat ttttcattac ctttttataa ttagtggcat tatatccatc
     3361 ttgtgtatct gaaacatcta gttcttttgc actttcagat atattcccaa aaccatatag
     3421 cgtgttagaa gataaataca atagagcttt atataaaaat atggctttat cagaggtaat
     3481 aggacctgaa tttatattag atgctgcaat attattagat gattgattgt taagataatt
     3541 ccttccatat tgatgtatat aagatgaacc tccagaaata agtaaattat ctggattgac
     3601 attttttaaa ttacttgacg attccccatt atataaataa gcaaaagttg tagaagatga
     3661 tgattgtgta catcttgtag gataataatt atatatataa gaatcaagat atcctgcata
     3721 tgaaattccc tgatattgaa ctctatagct tgaatcagtt ggttttgtcc atgaagtacc
     3781 atggtatcca gttagaaaag atccaatgtt tgtacaaaat acagttacat actcatatat
     3841 gataatatta ttaattttag taatattatt cgtgcatgaa actctactat aacttgaccc
     3901 tgatatattt atataatacc aatcacttaa taaacctaga tttacattag atccatcaga
     3961 agtactaaaa cctaacaggt aatttgtatt tataccagtt gcatatacct ttttttcatc
     4021 aactacaata aatgttgcat tatatgaact ataagcatca gttacattac taaggctaat
     4081 ctcagtatat tgacttatta atgcattact aagtccatta acataacttg aactctttcc
     4141 agaagtgtat atttgcttat tattagttac aattaatgaa aaccagtttg ataaatcagt
     4201 atttataaca acttttttta cattatctat agatattttc ttaaatacat ctgaattaga
     4261 aggaagaaca cctgctattg agttactatt tggtccagaa taataacaag tatcatcatt
     4321 tttaatgtag aatattcctc cagaaatatt ttttgatgct actatagact ttacattaga
     4381 gatacttaat tttgtcaact catttaattg acttggtaag tcagaaccca ttcctttgta
     4441 tctattattt cctagaccat atacatctcc attccctaaa ataaccagag tacaataagg
     4501 actaaaaacg acatctctta cagaatgtat atctagtgga agttcaatat cctttatttc
     4561 catttcttct tttaataaag ttaatttatc ctcagattta attaaattta tataattact
     4621 ataactatat ttttcactat ccattttctt aaattctaca atgtgcatat catcaaaaaa
     4681 cgaacctaat tgtccataag tattatctcc tgtacaatat gaatcattat ttgaatcaat
     4741 tagtatgcta tgtgataaac caatgaaaat attcttagct acaatatttt ttgaatctat
     4801 aaatgaatta tctatattct cagagtaatt tgctatacct aactgatata tatcattatt
     4861 tccagcaacc tttaaagtac ctttgttatc tataagcata gtgttaccat gaccacatgc
     4921 aatagttttt atatttactt cttctatctt agtaaatagt gatgattgaa gtgcatttcc
     4981 aaaacatttt tcctttccac aaccatatat atcattatta tacgtcatta acacagtgtg
     5041 ttcagaacca caagctatag tcttaacatc attaatagaa actaaactaa ataaatctct
     5101 attattctta tctcctaaac ctagttgacc ttgattatta aaaccagtag aatagcattt
     5161 attgtcattc tttattataa aagctgattt attatatgaa aatacagcct ttacattgtc
     5221 aacatctact tttgtaaatg tatttacatt tgcagatgct ccattagttc ctagacctaa
     5281 ttgaccatac aaattatttc cagcaacata taaagttcca tcattgtata aaagatatgt
     5341 tgattgaagt ccacatgcaa tatcctttac gccttttatt tctggaacct tctcaaatgt
     5401 agaaactgtt ttccctactt tttctaatcc caatcccatt tgaccatctg tatttatacc
     5461 tgcgacaagt aattctccat ctgaagtaag tataactgca tgagattctc cacatgcaac
     5521 ttttttaata ttggtagata tatttggtat tttcgtaaat aattttcttg ttgtattgtc
     5581 ttctcccaat ccaagttgaa agttactatt atctccacat ccatacaaga tattattatt
     5641 ttttagacca aatgtatagt tacaacatcc agtaaaaaaa ttaataaagt cataattatc
     5701 atacccttca tcaaattcat caactaccat atttttatcg ccaaagaaat tacctctttg
     5761 aagtagttta gttcttttca ttataaaaac ctcctaatta tcttatttca tacgaatatt
     5821 tatttaaagt tatatctgtc tgaccaattg aatatgccct attagatttt gtttcaacaa
     5881 agttattatc catttcatgt atatatctaa ctctatttgc attaaatggt agcaccacat
     5941 tcttaaatgt aacactacca agttctccag ttatacactt tctatttaaa tctaacttaa
     6001 ggccttctat agctattatg tttttagcat tagttaaggt atcataccaa tatccagctt
     6061 catctacatc tatttctgac tctaattcta agtatattaa gatatctaat actttttcag
     6121 ataatttatt gtaactattt tttaatacag atagttcagt ttgtatttga cctcctatta
     6181 catcatcaat agatatattt ttaatttttt ctactacctc atttatagct cctgataagt
     6241 ttttagcttc tgtagataag agttcttcgc caatttttac ttttatttct tttatatttt
     6301 tatcaaactt atcatctaaa tcttgaatat ccttttgagt tacaaaaacc aacgtcgggt
     6361 ctacttctaa tgtaacattt gaagtattgg acaattgtaa aatcattttt acaaccaaat
     6421 ctttaatact gccatcttca gcacgtggct tatacgtctc tggatactta cctatagcta
     6481 ttatattatt ctcattatcc agaacagcag cttctcttat cataaatcca ccaactgttc
     6541 ctggtaaaat agtctctata actatccaat taggattttt ttcatcaatt cttagagaat
     6601 ttatatttcc ttcccaaact acattcttta aagcagtttg ttcctctgtt ggctcataat
     6661 attctccatt actatctcca acttgaaact tggttaatcc aacaagctct cctagtgcag
     6721 ttgcatttgc tattttttct ctaccaattt tggttaatat tatataaaat ttttcttcca
     6781 attataccac ctccgattta ggataaacta ctacattttc tacacctgta tcatttgcaa
     6841 tagctatatt aatcttacaa ttattttcaa tgcatttaat gcttaaagga taaaccttta
     6901 tttcttctct acttaacatt ccagctccac agtttacaac agcactacat tttataattg
     6961 gttcaaagta tggatgaact tttacctctt cactacatac catacctcca ccacaataga
     7021 ccatactttt atctagtatt attggttcta aatagtgtaa taagtgagca ggttttactc
     7081 tttcaatcat atcgctacaa tctaaaagag ttttatagtc acagttattt gctataaaac
     7141 tcaatacgaa tgtaaattca tcactataaa cttttatatc tgtttctgat tttgtatatg
     7201 cctcacatat actttttata acttcaatag tactagtacc tctgcttctc attttagctt
     7261 ttatattgct acgtcttgtg tcaaaatcaa gttctttttt ttcaatgcag agtatgtctt
     7321 cccacatatc taatccccat gttgcagtgt caacaaataa ttggttaaag gtatcatcaa
     7381 tatcttcttt aagaatattt aattctgtat cgtatgcacc ttgtatctcc tctacaatgt
     7441 aatttctatc aaatgatggt agtttatcaa ttagcttcat tgattttcca cctcactaaa
     7501 attaacagtt gttacactag gtattttatc ttcattaata gttatattat ctgtacttcc
     7561 atttataagt agcttactta aatcgtgtac accagtagta tttataagta ttcccattac
     7621 ttttgtatag attatttctc ctctaatatc tctaaggtat gtatttatac tttctaggaa
     7681 agattctttt acattgtcta atgtatatcc atcttctagt ttcattactg cacttatact
     7741 tatttctatt ggtaatggtg tcacaactgt tatagttggt ccaataggct tctcttcatc
     7801 tatatgttgc tgacaccttt caatagtttc tgtatcaaca gcttgattat ttttcccaaa
     7861 tatcaagacc ttaactgttc ccggaccatc ccatcttgga taaaccttaa cattatagac
     7921 tccatctact tccaaagccc attcttcata gtgagcttta tttccacttg tagcttgatt
     7981 tctttggatt ttataaaatc tttcttttag ctcttcatct gtctctatat ctgtaccacc
     8041 ttcaaaactt cttgtgttag taatctttgt tactccagat atattatcaa ctagtttaaa
     8101 ttcacaattt gtaggtaaat tatatttctt accaacttcc agagcttgaa ctgggctatt
     8161 gtcaccttct tcactaccaa tagttacatc ttttattact acaaatagta aatctctata
     8221 tgatattact gtgccattat ttattacagt acctttctct ccaataaatt caacttctcc
     8281 atttgactct gtacctaact ttctatatac accaaattca ttgactcttt tatcaagaaa
     8341 ctggttatat gtgtcttgta taaaagccat tttatgtatc tttgaaagtt ctagatatat
     8401 cttcgaaagt tctagattat ttccagatac catgttgttt agaaaagaac cttctccttt
     8461 ataaatatca agattaatat tttcaagagt tctatttttt attacttcat atgtctggtc
     8521 actatacatt tatttccacc tccccataga tagtggatac ttttacattt gcactcaata
     8581 cgtctctgtt aaagttagca ctttttacgt ttacttctaa tatatatgga tttattagaa
     8641 gggcctcttt tatgaatcta ctagcttcac tttctgtaag accttttgta tatttttgtc
     8701 ctattagttc tgaaagctct gtcccatatt ctaaagagta tatctcatgt tcatatctat
     8761 ttgtctttat acacttgtat atccaaactt ttaaggcttc ttttttttca attattttaa
     8821 agtcaccttt ttctaaaata ggttcatctt tttcaaaatc ccatgccact tcacgaaaga
     8881 ttggcaattc ttctgtttta ggtaagatat aatcctctgg gacacctata aaaggaaata
     8941 ttgtactcat tataagctca ccaacttact tacaacagca aatttttcgc ctattctaaa
     9001 cattattacc atatccccag attcaaagtt atctataaaa ggatttttta cttcatgctg
     9061 gtgttcttga tttgtttctg tgtcaaatgt ttctagctgt ctatcaatca tccaactatc
     9121 tatcaaaata tcttcttttt ctaatatgat gttatttgtc tctattttta aatctggtaa
     9181 tttactttta atttttccaa taaaaaaaga aggttgatta tgatactttc cttcttctct
     9241 tattatccct ataaattcat ttattggatt agccattata tcaccatcct tttataatac
     9301 tctcctagct gtattaaaat ctcttctact acttaactta cttattttaa ctacatcacc
     9361 tttttgtgga gcatgtaaaa attctccatt acctatatat aatccaacat ggctgactgg
     9421 attatgaaaa aatactaaat ctcctgcttg aagatttttt tgttctactt tcttgccttt
     9481 tttagattgt tgatttgacg ttcttggcaa actaacatta acttttttaa aacaatagac
     9541 cattaaacca gaacagtcaa aactacttgg tccatttcca ccccatttat aaggtttacc
     9601 tagatgtttt tttgcttctg atattactat tttagcttta tcagatacat taccagaata
     9661 accaccaata ataatttttc cttttcttct tccaaattta ctagcttctt caacatttcc
     9721 aaataaaata tctatatggt atgttccatt cttttctata gttattgcag gtcctacgtc
     9781 atttacttta tagactccat cacgaccaga aactcctgta attacctgta tttcatctcc
     9841 atagtttaat aaaggatgtt tatttaaaaa ctcttttgta taataagttt gctcatattt
     9901 accaaccata ggagcagcgc aagttttttt agatgggtca agttttttct ttctacaatc
     9961 tgtatctcca ccttcttctt ttctaggaca gtaagctgta aattctgctg taaactcttt
    10021 tcctcctgca taatcttctc cccctaaatt actttcttcc ttaggttcat cctgtcctgc
    10081 tgacttttca tccataagat tttgaaaatt aagttcaagc tcaatttgat attctccatt
    10141 ttgccaagta tgtttgtctg tatctatata aaatagtcct acaagctttg tataagaatc
    10201 tttaactttt actcctctac cagttataca acttacatct ccataacctt taagagaaca
    10261 gcttttttct atcccattaa actcgctatc aatatctaca tcttgatttt cttgttgctg
    10321 aattactttt tgcattatta catttacttc cttaaaaatt tcattatcta ttttttcgct
    10381 aatcttgctt ccatactggt ctaccactat tactttattt tttacatttt ccatgctctc
    10441 cgaaaaggtg gtatttataa tattaaatcc ctcttcaaac ataacattta atgtaacaat
    10501 tcctttttca ataacattaa acttatctaa attggcctct atcatatact ttttctttgt
    10561 ctttttactt gcttctgtat atgcactcat tattgtgtca taaccattta ctcctataaa
    10621 catctttgta tacttgacat tggtctttgc tattgttcca actgaaagcc tattttcagc
    10681 aaatacttgc tttgctatgt cttcaactaa cttatcttta aaattgtatg acacttcact
    10741 ttgtgtaagt aaaaatccca tatctttaga tacaaaacta atttcattac tgcttgaatc
    10801 tttagaccta ttaattatca ttcctctaaa gagttctttc tcatctacat agaaacagac
    10861 tgtactagct attggtatat ctatttgttg gaaatttaca tcaaatgatg attgtattat
    10921 tgaaaactct agtgtccttg atggagattt ataatcacct gaccatgata ccttgtctac
    10981 tatgtcagtt atgtcatata tacttccgtt ttttatgtgt acccaaatct taataataaa
    11041 aaatcacctg ccttttcatt taattctata agcagttatt tataacttat tatctataat
    11101 agttattgtt ttggagttac ttttggtatc tttattcttt tatactcttt taaatctaat
    11161 gtaaaatata catctcgagt cccatctctt tcttcataac taaacccttc aattatgact
    11221 tccatattta tatttgtttc cgtaattgta aatcttaata taaaaccttc cttcatccat
    11281 ttttctatct tatttacaca atcatatggt gatggaaaac ctgtatagtc acagaaagta
    11341 tagtctttat ttggaaaaaa acttgatatt gaaatgcttt taagagctac accaccaaaa
    11401 gttgctattt cacctatttt gagtatacta gaagagttta cagcagcttt tccattaata
    11461 ctaaaggaag atggaaatac tggaaatcta aatctatctt cagcttgtct aagccacatt
    11521 tccattataa gacaccccct aactgtgcca attggatact tgcaactaat tttgatgtta
    11581 tcttttctat atcagcttct tctctaatca ctattgtatc agctaactta gctatgttta
    11641 tatttccacc attttttctg cttctatatt gatttgcttc ttgtttagtt aaaaccattt
    11701 caccttcatg gagtctggtt tgatagtcat tataaggaac atagttcaat ccaaatgcat
    11761 tattaccttt cttacctgaa taagctttac cagaatcacc agcctttgtt ccagcatatg
    11821 gattatcttt gtaatttttt ttactctttt ccactagatt taaaactgtt cctgtgatgc
    11881 ttccattttt tatcttatta atcatagttt gccaccactt gcatatatct ttaactattt
    11941 tacatacttt atctaatatg ttaaaaaaag ctcccaatat tggctctata attttccatc
    12001 cagtttccaa cagaggtcct aaggtctccc atacagtttt ccaaataaca cccagtgtct
    12061 ctataaaatt gtttatttga ggtgcatatt cacctaaaaa agtaaaaatt cttgtgataa
    12121 taggactaac agcttcaact acaggtccga ttagttggaa tgctgtagtt actattgtac
    12181 caaatatact tattaatcct tgtgcaattg gtgatttatt gtttatacta tctagtaaat
    12241 tatttactaa gtttactact ggaagtaatg aatctattaa tgctgtaaaa tcgatatttt
    12301 gtaatagtgt acttcctgcg ttaacaattg cttttggtgc attccctatc attgttgctg
    12361 catgattttc aaaacgttct tgcttatttt caaccatcat tttacgcttt tcagcctcat
    12421 tagaaattcg atattgagta gaatcagaaa actcttttcc attttttaca cttctagatt
    12481 gcatctggtc atatttgttg ccaataatta tcttaccatt cattacattg cctattttac
    12541 cagccatttt ttcagataag ccagctaaat tgcctaatcc actttctata aaagttttta
    12601 ttttctttag tccttgacct aaaatatcat caaaaggttt aacaatactc ttcaagccat
    12661 tgttaatgct tgtttgagct cctgatatta tacctggtat tgtattactc atttctttag
    12721 ctaaccccat ctgctttaca cttatgcctt ccaatgcttc atcaaaagtt ttataatttt
    12781 ctcctaccag agtattaaga gattccatgt ttccttttgc tgcacttaag aaagctgctg
    12841 atacatcttg ttcactactt gtattcatat ttaaagctct aacatctacc atagcttgag
    12901 ttatatcttt ttcttttttg ccaccagtaa tattactaat cattttagct ttagttccca
    12961 ttgcaacaac atcttcaggg tcaaaaggcg tatcatttgc cattcttact aaactgccat
    13021 aataatcatc aacatctatt tgattaactt ttccagtatt tcttttcatt acactttcta
    13081 gaacaacttt ctgattttgt tcctgaccaa gtaaatcaac accctttttc acagcgacac
    13141 ctatagtagc accatttgtc aaatcagata tcttcccttt tatcgaacca agtattccac
    13201 ttgccatatc ctttacagct attgcaccac tccatacctt acctgcaaat aatctcaatt
    13261 tgggaaatac tttacttata atgccacttg ccatatcttt tatagctatt acaccactcc
    13321 aaaaagtatt tttaaacaac attaacttag gcaaaattct acccatagca ctactcacca
    13381 tatctttaag attaatactt ccttgataag gacgtttctc tacttgtttt aattttccca
    13441 agatactact tatcactctc ttagtcatat cttttgcttt tattacgata ttccaaatct
    13501 tgccaccaat cttttttaaa tttccaagta atttattttt agttttttct acttcatcac
    13561 ttttagcttt aaatattaat ataatcataa tatttgaatt aaactcattt ttcttttgaa
    13621 tcaacttatt aaaataatca ataatcatag ctatagttat tgctagtctc ataatacttc
    13681 catttaaact tccaataact cctgaaacag catttatgct agaacttaat tttgtcatag
    13741 ttgaacttac actactagca gtagtttgaa cttttcttaa cgaggaattt aaactgttta
    13801 ctctattact tgcacttcca atagaatttt ttatgctact agccattaca ttagtggaaa
    13861 gtgaaacagc cacagttgct ctcctaaaat tatcagttac tgatataatt tgattcattt
    13921 ttggtgtgta attatctgta aggtacatat ttataccttc ttctctcaca tttcccatta
    13981 cataccacct cctgtcaaag gacacactcc atatccatgt ttcatttttt caacttcttc
    14041 ctcaatttct aaggcgaaaa aagcttcaat aagctttaat tcgcctttat ccattgcata
    14101 aaaaagggac ggtcttatac cttttttctt ccaataatag tacattattg tagtcctggt
    14161 atccgtccct attagttttt tacctcttct atcactgcat ttttaccata acccataaca
    14221 tcttgaagta ttctatataa gttggtaatt tcacctggta ataacaattt tcttgctaat
    14281 tcttttggtg ttggagcctt aaatttatcc attagctcct tatttttaaa taaaagcttt
    14341 ccatccaaat cacaaacacc ttctataatt gtaagcatct gcatctccgg taagtctata
    14401 tccgttttca tattttcatc aattttaaga caagtatttt gtatttcttc atctttactt
    14461 attgttaaag ctctacacat tactttaaat tcctgcccaa atatagttga taatctctta
    14521 agttcaacta tagtacttgg tctttctaag ttttctgtat ctgcatttaa aagtaaatct
    14581 actatgttta tattttttaa taatccattt tcactcatta ttatttgctc ctttatcaat
    14641 caaattcaat caactaactt ttttacttat taactatctg aatttattaa gtatatattt
    14701 actaaatatc agtataaaac caaatataaa actaaatata aaactaaatt atatcaagaa
    14761 aatcatactc agtaaatgtg aaaggtgctt ctatctctcc ttttacgcca acctcccaat
    14821 cagctaaagt taaatcatca aatgctatat tctttactgc tattctttct gctccataag
    14881 aatctgggtc atttaattta gatatagcaa caaatctagg ttcagaacct ctctttatct
    14941 tttctccaat taacttgtgc attcttgaac taacatggta tagagttatt gagccttttc
    15001 ctttatatcc catatacttt gtatcagtac ccatttgacc tgctattata atatcttctt
    15061 ttgtaaattc catctttgct tgaaacttct ttacttctgc tactttgttt ccatcaagcc
    15121 aaagttctcc ccaagtacca ctcattacat ttctagcttc catatttgcc atattaataa
    15181 tcctcctata tttctattga taaatctata tcttccatag catcaagtac ttttattttt
    15241 gcttttaaaa atactttaga acctgtgtta gcttctttta tttcttgtaa tgtcatataa
    15301 gataaatcta ctccttttga ttttaaatac gatttttgtg cttcaaaatc tatttcaaca
    15361 gtagaatcag attctataag tgctgatttt tctaattctt ctaaataact ttttatagct
    15421 actatcaata aacatttgtt gtcataactg ttagtaacct ttcctatata gtcatctatt
    15481 atcacctttc ttatgtcact atgtataata tctaaagtgt caactatttt tattttctgg
    15541 aacatttctc ctttttctgc tgttaattca gttaaagaat ttactcctct agcaattctt
    15601 atagctcctg cttctttaat aagtataagc tctcctttat taacccttga ttctgcatca
    15661 actttcgtca tcttaggtat atcgactaca tcactaagtt tagtataagt tactgattga
    15721 cttaaaggtg tacctgctat aagtccagcc accctacttg taaactcatc aacactgtat
    15781 ttcttttctc caactaacac atcttctgta gtaaaattaa ttatcccttc atgatttcct
    15841 acaacttttc ctaatacagc tttaacctta accttatcta tatctctaag tttaattatc
    15901 caatttttta tagcagtctt atcagcttct actgcttttg gcatacatag ataattaaat
    15961 tccttagtct ctaaaaaatc taatgcagtt tgaatatctg cttctccttc tattacataa
    16021 actaataatt tatttggagt gttaacattt cccatcaaag ctaaatttat atattcttta
    16081 ttttcagcag ataaatcaac tggtatatcc tctttttcat gtatttcatt aagacctagt
    16141 gccttagcat ctttaagcac cattgcaatt attcctctag ctgaacgttc tttaacagtt
    16201 gtagctagct ccttaaatga tatgttgata cttggtaatc ctatagccat actatcactc
    16261 tcctaaattt aaattgattt cttccatatt ttcatatact acatcttctt catatacact
    16321 atccagatag tttagtgtaa atgtaaagtt caatacataa attgactctt cttttttaat
    16381 actttgttct agctttgtta tatttaattt tctgtctgtt acctttatat ttctgttgaa
    16441 tagcttattt aattcatctg aaatttcata cattctaata ctctttgact gtggttttgg
    16501 taagtatttt atagagataa caatatcttt attattcgta tttaacatta taggtgtaca
    16561 aataactgat agtatactta caaaaaaaca agacccttca gagccttgca cactttcatc
    16621 atcaataaaa atactttcag taaagttttt tttgagaatc tcaataattg tttctaatat
    16681 ttccttgtat ttaagcaaat aatcaccaca ctatcactaa atattcttgc ttaatctgat
    16741 aatcttacta taaatgatat aagtcatttt taaatatata tctcatttat tcctgtcaag
    16801 tatcttctat atttttaggt aatattattc ttttcaatct atcacctcct tcaatatgga
    16861 tttctttcac aataacatta tctcacatat tttattgcct attgttgcaa tggtgtatat
    16921 aaagtgttgt aaaattgtcc tattttttat aatatctcac tcaatttaaa tatcatcttt
    16981 ttctttaaat tgtagcaaga tgttctatct aagtgtagtt tcatagaaat atatgtcata
    17041 ttgttttttg tcttactatt ataaaaaaga ttaaaaaatt ccatttctat atcatttaga
    17101 cacgttagag cattttctat cttctctttt tctatttcta tatcttcttt ttccattttt
    17161 aattttctta atctttcctc tctttttaac acctcatttt ccacagaaga gcttatgtta
    17221 tatgtgttac tagtcttttc tgtatatacc attgctccac atccagagta ttcattctca
    17281 catcttttta tgtccaagtt aatattcttt atctttatat ccaaatactt ataattatgt
    17341 aatcttttat ctgtttcttt aaataatata tcttttttgt tttccatagt acttctcctt
    17401 ttttatatac ttattattag cttcaaattt acaataatct ctttagtgca cagtttttta
    17461 ctaatctcac aactttcaag aacatattgt tctatgactg tataaaaaaa acttcttcta
    17521 tattttgttc taaaatacta gaaatcttct tagccataag aagtccagga ttttttatgc
    17581 ctctttctat ttgagatata taatcatttg taattccagc ctttctggca agttgaattt
    17641 gtgtcatacc tttgcatttt ctataatatt tcaagttatt tttaaacatt gttttatacc
    17701 tccattacta ttaagaatat tttgttcttc ctcacttaga ttatatagaa ttatttgttc
    17761 tttatcaata tattttagaa aaaaatattc ttttatattt tattagtttt attttttaga
    17821 acaatttgtt ataatctata cataaggtgg tgttataatg tttgccaaaa gattaagaga
    17881 acttagaaag gaatttggat tgactcaaag agaacttgga gaaaaagtag gcgtttccca
    17941 aagagtctta gggtactatg aaacagaaaa cagatttcct gatgaacaca ttttaaataa
    18001 attggctgat gtatttaatg tatcagttga ttaccttctt ggaagaacct tagttaagga
    18061 aaatattgat acagtagctg cacatagaaa aaatccacat gaagagttac ctgaagaagc
    18121 acaagaacaa cttaatgatt atatagaatt tttactaaat aaatataaaa aaaaataaac
    18181 catatgatga gcagtctatt tttctgctct tttaatataa tttaaaggca aacatacatt
    18241 ctattttagg gggattttta tgaataattt ggataagctt tttgaattag cttctcaaga
    18301 agggataata attcattata ccacttatat tgcaggtgat ttagaaggac tatatataaa
    18361 caaacatggt attaaaatta tatcattact cagtaattta aaacaaaact caaaaaaatt
    18421 aacatccatc ttagctgaag aacttggaca tcatttcact agccttggat actatgtatc
    18481 ctcttataac gattattata caaaaattat tatagataag tgcgaaaata aagccttaaa
    18541 atgggcttgt gagttcttaa ttactgaaga agatataata aacataatta actctggcat
    18601 cacctgcgtt tatgaaatgg cagacatact taatgtggat attactttct ttcaaaaaag
    18661 attagaattt ttatcattaa aaaaacagtc tttacaactt ggaaataata aatatttaat
    18721 attaactaat ttgccgtatt tctacatatt tgatccaatt tcataataaa aatagtagta
    18781 attttaactg gcttaacagt aaaatctata tatttatttt aacaaatttt ggtttctata
    18841 aattcaatat taatatataa taatgatatc atattattta aaattctaaa aacaaatata
    18901 tgcttttgtt aagaaaggtt gtttgatgaa tgtataaaga aaagttgttt gataattatt
    18961 ttaagttctt agcactatta ttttggccaa ttatgtggta taagtggata gtaatttcaa
    19021 atggtactct tgaaaatatg ttatttacca tatatgcaat cgtcgcaatt gttttcatca
    19081 ttttatattc ggtgtttatg ataaaatata aagacattac acaaatagat ttcttttaca
    19141 agataagtac tctacttgct tttatattta cactttttag ctttttaata tacccaaaga
    19201 gtttattttt cttgtattta aagattattt ttactggaat atacctttat tattcaatag
    19261 taaaaactct taaatttaaa gatgacgaag gtgtagtggg tattatgagc tcacttttat
    19321 taattgtaat aactttgttc tactaaattt aattctattt gacttaatat ggctttaaat
    19381 attagacatc tacattattt tctgatatta tttaagatgg tagtcttata ttaataatga
    19441 aatacactat gaaggagtta actattatgg aaaaaaaagt tttagcaaca gttggagaaa
    19501 aagagataac taatattgat attgaaaatg cattaaaatc attagaccct tatcaagcta
    19561 tgcaatttaa aactgaagaa ggaaaaaaac acttattaaa tgatttagtg aatcaagaat
    19621 tattcttctt agatgctaaa gaagaaaaat tagatgaaga agaaatattt aaattagaaa
    19681 tgaaaaagat agaagaaaat gtattaaagc aatttgctat aaataaagtt ttatctagtg
    19741 ttaatgtaac agaagatgaa aaagtaaaat tctttgaagc taataagagc tctttctcta
    19801 aaccagaaag tgcaactgct aaacatatat tagtagatag tgatgaaaaa gctaaagaaa
    19861 tacttgctca aataaaatca gaagaaatat cttttgaaga tgcagctctt aaacattctt
    19921 cttgcccatc taaagatatg ggtggtgatt taggaacatt cggtagagga caaatggttc
    19981 ctgaatttga agaagctgtg ttctctatgg ctaaaggtga agtaagtgaa ccagttaaaa
    20041 ctcaatttgg ataccatata ataaaattag aagaccttca agaaagtaca gagtctacat
    20101 ttgatgaagt taaggctgaa gttgaaaaaa gtttattata tcaaaaacaa aatgaagttt
    20161 atggaaataa gataaatgct cttaatgcta agtacggaaa ccttgtaaaa tacaacgatt
    20221 aatcataagg tcatatataa aaaagctgtc ttttcacaat atttattaga aaagacagct
    20281 ttttatatta aagtttaaat attcagtttc atattatcat tcttttattg taaagaactc
    20341 agcttaaaag tttaaataag atttttaatt atcattagct aaatttttat atccacgtat
    20401 tatctcataa cagtaaccta ttacatctct tctttttatt atcccaataa atgtgtcttg
    20461 gtcatctata acaggaatga aattttgatt taaagatttt ataactaaat cttcaatatc
    20521 tgcgttgatg gaaacaggag aattatccat ttttctattt atatctgtca ctggaacatc
    20581 ttccaatccc tttaagtcca gatttaaatc attttttaat gtccataaaa aatcaccttc
    20641 tgttatagtt ccaacatatt taccatcctt acttattatt ggaattgcag agtatttgtg
    20701 atattccatt ttttctaggg cttgccttat agtgtagtct tcatatatat atgctacttc
    20761 actttttggt gttagaaaga ataatatgtt cattacacta cccaccgtta ctgaaaactt
    20821 ataaatatta ctcaatattt ataatgttac ttttttgcag taactttctc ctttcttatt
    20881 ttctaagtat tcttaatact atacgctgta acaagcgttt ttcctttgct cattttaatc
    20941 ttattatgct ttattaatat ttttatatgg tctatcagta tcacttattc tctttttaga
    21001 aaatttacta aaaattaatt gacttaaact tagccataat aattttactc tacactatta
    21061 attttaacat atttatctca ttattaatga ttttatttta ataattttaa tttattttgc
    21121 tattaatttt atttacaaaa actgaaaaaa ccgctaaatg attagcggct tatctctcat
    21181 attactagaa cactaatata tatgaatttt taattataat ctaactacgt tagcagcttg
    21241 aggaccttta gctccatcta ctacttcgaa ctcaacgctt tgtccttctt ctaatgtttt
    21301 gtatccatct ccagttatag ctgagaaatg tacgaaaaca tctccttcac cttctacaga
    21361 tatgaatcca aatccttttt cgttgttaaa ccatttaact acaccagttt tcattaataa
    21421 atcctcctaa aaaaacacaa atatttagct actcttttct gtaaccttat tactactcta
    21481 tcatatatag ctatgtttgt caattattat tatttttata cattaataat caattttttt
    21541 actttataat tttaaatgat tattatcttt tacactttat ttttctttat aattttttaa
    21601 taatttattt ttattatatg gaatcttatg gacttatcac tccaacctaa atcccatggc
    21661 accaacattc tatcagtatt atgacaagtt accaaaggat accctcttga gtctaatcct
    21721 gtcactgtcg aaacatgtgt tattctgcct ttcttctcat aaccaacaaa atcccctgga
    21781 cgtaaattat aagcttcttt gtatacttct tcataggaac ctttagcaat taatgaacct
    21841 cttccactat acagtgcata atttttaaaa gcttgtgcat ttacccatgc ttttgtacct
    21901 gctctctcgc tataattcca aattgagttc tttttgaacc ttccactttc aaacattatc
    21961 tgtgatgcaa agtttgcaca atctccacct tctggattat aatccctata gtttggatta
    22021 aatttcatac catgttcttc atcagctgcg gctccacagt atttatgtgc ataatctata
    22081 gccttttttt gttcatctgt aagctgtaac tctacaccat cttgttgccc aatatagttt
    22141 cttatatcct cagattttat attttctaaa tttaaagagt ctgcaaaagg gtctgtgtac
    22201 cactctttag ttatgacata ttgattatct ataattttta catttagata atgatatgta
    22261 cctattctaa aggcattttc tttgtccttt tgattttcat atgaatactt atattcagtc
    22321 gatactgaac agattatacc atagaggtct ttttcttttt cttttatttt tttaatctta
    22381 acccttgtct ttatgtcttt aaattttaca ccttgcttac tagaccagtt ttctaaatac
    22441 ttcatctttt ttacttcatt ttcataagcc catagaccaa attttttatc cgtctcataa
    22501 agacctttta aaatattttc atttttctct aatattgcct tatttctgta gtcaaataag
    22561 ttttcaagca atagtttata ttggtctttt acatcatcac cttcaacatt tgcagattcc
    22621 atgacttttt cttccatatt tttcatactt aaatcattga ttaaaaatac acctgctgta
    22681 gtagctaaag acaaaaatac aaatgccatt atacctttga tgtacttatt cttatttttc
    22741 agtattttca aagctacacc tcccaaatag tacaccttac aaatcaaatt acaaagaata
    22801 ttctgtcaca tatagtaatt ttcatattta ataattctct attttattat gtccaatatt
    22861 aaaaattaat tgtatcaata cactgttttt tttacaattt tttattttat aatctacttt
    22921 tttctttttt ttatagtaat atatatgtat ataaatattt tggtatcatt ataattaata
    22981 catactaatg gagggttttt tatgcaaaaa aaagtcgcag caattaatga cttatctgga
    23041 ataggaaaat gctcattatc ggttgctata cctatacttt cagcattaaa ggttcaatgt
    23101 tgtccatttc caacggccat actttcaagt caaactggtt atcctgaatt tactttctta
    23161 gactttactg atgaaatggt aaaatattca aatgtttgga aaaatttaaa agtcaatttt
    23221 gatagtatat atagtggttt tttgggttca aagcatcaaa ttgagatagt ggctaatttt
    23281 ataaacgatt atcccaatgc ttttatcgta gttgaccctg tgatgggtga taatggagtt
    23341 atgtatccaa tattcacaga agaaatgaga caggaaatta aagaattagt taaacattca
    23401 gatttaacta ctcctaattt aacagaagct tgtttcttaa ctggaaatga ttataccaaa
    23461 agcgattata atagagatga gcttatacat attgcaaaat ctgtatctga tttgggacct
    23521 agcaaagtcg ttattactgg tatattagaa gatgataata tattaaattt ggcttacgat
    23581 agagataatg accatgtatt ctttacatca gtcaaatata ataattgttc ttacagtgga
    23641 actggagata tattcacatc tatactttgt ggtatgttag taaataaaca tgatttggga
    23701 gtagcggtta atactgcaac agattttata tataaaacta taaattacac atctcaattt
    23761 gatactgata gaaatgatgg agttatgttt gaaaactttt tatcagattt aaccaatata
    23821 taaaatcaaa aaaactatcc attttataaa tacctataaa tcataaaatt taggtattta
    23881 caaaatggat attatttact aaactattgg tatttcaatt gttaccatag caccaccagt
    23941 tttcacagaa ttttcaatac ataaagtccc attatgtttt tgtacaatat tatttacaat
    24001 atagagtccc attccataat gagatttaga atttctactc aaatctcctg tataaaactg
    24061 ttctgtagct tttgacaaat ctgctttaga aaaaccactt ccacaatctg taactacaaa
    24121 ttttaagtat ttattacgta ctgatacact tatgtatagt ttactctggc ttggtgaata
    24181 atctatagca tttgaaatta tattcataat agctctgaat aacagttctc tatctatagt
    24241 tatattttta ggcaaattat cctgtttata aaactccaca tctaagtttt tagtactagc
    24301 taatgcttct atttgcacta agacatcttt tataaactca ctcatactga ttgcttcttt
    24361 atttaatata tagccattct ctgtttttga tatatctatt aaagtattaa tatatttttc
    24421 tatttgagtg acaccctttg agatatattc catgtattct aataactcag gattttcatt
    24481 tatttcaatt aataagtcag tatttccatg tataattgtt agtggtgttt ttatatcatg
    24541 tgccagtgca gatatctgtt cctttctatt ttcttctagc atccattgtt tttctaaaga
    24601 atcttttaac gccaacttca ttttatccat agaaaaaagg acattattaa tttcataaat
    24661 tttactagat tctacaacaa aatctaaatc ctgttgttct attttttctg tagtattttt
    24721 taaaagctcc atttcaatat taaatttctt gtcaaatact tttgataata taacaatttc
    24781 aataaaaaat attatactaa aaataatcat acccaatgtc tctggttttg gtaaatatgc
    24841 tcttaaaatt ggagaactat actctgatac tattttgtat tttattataa aaacttcatc
    24901 tttcttaaaa aactttatat aactttctga atatgcatgc ctttcttcaa tatctctcat
    24961 taaattccat acttcttttg attctttttt attaaaggta cctgaaatta aattcccatc
    25021 taaagtataa accccatact catatatact tggaattata tcttcagtta ctttttcact
    25081 tgaaataatc tgttctttat atttattaaa ttgtttttct gcataattag ctggtaaaat
    25141 tacacctgat gataataata cagaaaatga taacattaaa aggagtacaa ttgaaattgt
    25201 cataataaag aataaagata aatattttat aaaaatagtt cttaactttt caactttttt
    25261 gttgttagat tttttacctc caagcatttg taccctgtcc tccaatagtt taattaattc
    25321 ataattaata cctagccagt tttttatttg attattttat atctttttat tgctaagtta
    25381 tattttaaca taataatata aataaatagt caatttttac tctattcttc tgatttttta
    25441 ccttcccatt tagaaaacca aatacataaa attacaaatg ctaaaattgt tacaaaacca
    25501 cacattaaac ctgttgattg atatgctttt attgattcaa cctttatata ttcaaccgtt
    25561 ttagaacttg aaaaagacga ccaaatagtt ataaatctaa caccccatgc acaaggaaaa
    25621 tactgccaaa tagcagaacc caatccagtt aataataatg ctgaaagtaa catttcaact
    25681 atacctactc caattgatgt tccttttccg aaccttaaac ttaagaataa atgtaatatg
    25741 tattcaaata aattacaccc aattaaaatt agaattgaaa tcatataaaa gcttgatgta
    25801 aagtaagcct tagatatacc aaatttaaaa cctaatacag acactaatgt tgctacaaaa
    25861 ccaagacata ctaagattat atatttactt ataagagtaa gatattttgg attagatgtt
    25921 gttaacatat gtttataatt ccctgcataa tattcttgtt caacaaacat tgaacacaca
    25981 acacttgaca ataaaggaaa agctattgct ataacttgta aatatgctac tatctttagt
    26041 atattatcag ctcctgaaac cttataatac cataaaaaca atcccatccc cattaaagca
    26101 atgcaaatgt gcattagtat tataaaattt ctctttaatt ttattaaatc agaacaaaat
    26161 attcttatta actgtctcat attacttcac ctctctatta ttgaaccaaa cccttgtagc
    26221 taaagcaaat aatacaaata aaacaataga tattaatact cctggcaaaa taacagcatt
    26281 atttaaaaat tcacttccct ctggtacagg aagaccattt ggcagaatat gcaatacaac
    26341 agccattaat cttgaaggaa tcgaatatgg aaatatatac cacatttctg tagttgagaa
    26401 aacagcaact ccaacaatag tgaagaacat atttattaga actgtaacaa aaataccaaa
    26461 ttttgaagct aaaaacatac aaagaggaat ctgccacata aatagtaaaa taattaatat
    26521 agttgcaatg atattttgac ctattgaaat agtttcttta tagattatcc caacaaaaac
    26581 tacacctatt gcagaaataa ttgatgctat aaaacagcaa ataatacagt aaatcatttt
    26641 tccagtccaa acatctttca tttcaattgg caatggtaaa ataccacgat atttactttt
    26701 ttcatctttt aatactatta aagaacatat taatgttaat gctcctggaa gaaatgtcgt
    26761 ataccaccag ttgaaagcag cattttgccc tccacctagt ccacacaaaa gtatacaaaa
    26821 cactggaact atccatatca acttcattcc aaaagttcct ttcatctttt gaaattccac
    26881 tctaataaat ctttccatat tactgccctc tctttctatt ttcagctaca actttcataa
    26941 aaagttcttc taagttttgt tcttttgata cttctccttg ataacccatt actccattag
    27001 aaattattcc tatttcatct acaacttgtt caacttcaga caagatatgg cttgataaaa
    27061 taactgtaat tccttgtttt ggaaaagaac gaatcaattc tctaagttct tcaataccaa
    27121 tagggtcaag accatttgta ggctcatcta agattaaaag ttttgggtga tttaataatg
    27181 caatagcaat accaaggcgt tgcttcatgc ctagagagaa ttgacctgaa cgctttttaa
    27241 aggtatctct taaatctaca gtctctaaaa cttcttcaat gcgagagtca ggaagaccaa
    27301 gtaaagtagt atggactttt aaattttctc gtgctgttaa attctcatat atagcaggag
    27361 actcaataag agaaccaata tttgataaat cctttctagt ccatctatgt ccctcaaaca
    27421 ttatttttcc tgaagttgga tttaaaatcc cagttatcat ttttaataaa gtagatttcc
    27481 cagctccatt tggtcctaat aatccatata tagaattaga tttaattgct agtgaaatgt
    27541 tatcaactgc catctgctcc ttaaattttt tagaaactcc ttttacctgc aaaatataat
    27601 tactcataat tttcatcctt tcttttgtca aacttattta caagaatcat tctaaatcat
    27661 aaatataagg attttataag aattaaaaaa caaaaaaaga tgtgttaaaa acacatcttt
    27721 ttttatctta attctattaa ttttccattg tagtatatct ctacttcttc aatccaaatg
    27781 ctattatctt cttcttggat gacattttta ataatattat ttatagtata tattatattt
    27841 tgttcattca aataatcatc tttccaagaa actctacttt ttttatctgg ttctatcaag
    27901 tttattttaa caagattatc tttttcctta acttctactt ccatgtctaa accattaaaa
    27961 tactcatttg ataatgtatc taagattaat cgcagtttat ccttaagtga actttcttca
    28021 tcaatctgta agtactttac tattttctca tcctttgaaa ttatctctat aggtatttta
    28081 gtagtttttt tagaattaat cattttacta gttgtttctt cttttgcatc ttcatccttc
    28141 tttacaactg ctgattcttt agaatcaact agctcaactg cactgatgct ataatccata
    28201 catgccgtac tcaataaagt agtagcaatt attaaaaaac ttactgctat tttattcata
    28261 agtatccctc tcctaattct ctttcgatac ttattatacc atagtataga ttatattttt
    28321 atacttttaa atcaatttat gaattattat agtaaattat ttacaattct aagctatttg
    28381 aatttatttt tatagtatat tctttctcta aattcgacag atttttattc aaaagccctt
    28441 ttatttccct cgtatttcta tacttgttgc tatgttcttc attatgttca taatcatatg
    28501 aatcattgtc atattcttca tcattattaa attcttcaat tgcttctttt gactttatat
    28561 cacaattaaa tttatactta tttactggta tatcaagtct taccttagaa tttggaactt
    28621 ctatgtccag gaacttagca attggcatat cagagtacaa gtaaaattca ctttcataat
    28681 ttgaatttct acctattttc tcttttatat ttacattttc aggtatatac tcttctatat
    28741 tattgataaa tacatctcca ttctctgaag atatatccaa tgactctgat tctatgttta
    28801 catttttaac gcctaaaatt gactttgttg atagatgaag attgctatta gatattaagt
    28861 ttaagttttc taatctattt actttatttt ctgttatagc tctgctaaaa cctccagaag
    28921 aagttttata tgttatatta tttgaatcta catcatcaga tataaataag tacgaactta
    28981 tacttgatgc atttatatct acattatttg gtacatatat tctaatttct ctataatcac
    29041 gtgaaaacat actatttata gatgtattta ataagtggtc gaaacttcta atctttttaa
    29101 ttttattttc agattttctt tcgcctttta caactaaagt cttatcttgt agggttacac
    29161 tgtatttgta aaattcatta ttccctcttg ttgtcacacg cacatcttca tttgaacttt
    29221 ttcttatcat aacatttata ttgtcaacag ctatatctaa cttgtcaata tttacttttt
    29281 cttgataaag tttgttttct ttgtttaatt cagcttcctt ttctaaacca aagtttatta
    29341 ggcttggcat aactaaaatc cctgaccatg ttgtccctat tatacctatt accaataaaa
    29401 ccgcagcaaa tatagcaagt tttttattca tgtttatctt ctcctttctt attttctata
    29461 tcttcaaggt ccttatatat cgcctcaaaa tttatactat catcattata ttcatcagtt
    29521 tcttttatgc tttcttcttc tctattatag taatcattat tatatgaatc ttcattaatg
    29581 tctttattca aatcttcatc atttaaagta gctttttttc ttttttccat cttttcattt
    29641 gcctttatat aaatattttt tctctttatc caatgtatat aattatttag taattgttta
    29701 cctagtttta tggcaaataa taaaatttgc catgcaagta tctcaagccc tacaaatgct
    29761 atggttgcga aaatacaaaa catcataaca tctggaacca ttgctataaa tttaatcaca
    29821 aatggaatta atataacaaa agcaattaat gagcatacta atgcaatagt agcagataca
    29881 atagcacata caaacgcaaa tgctggaaca attagaaata aagataacac tactaataac
    29941 ccctttataa cttgtctgga taatcctcct acaccttctt ctatacttgg tgtaagttta
    30001 tcatttatat tttcttttga tttattgtac ttttccttag cttcatcttt caagatagag
    30061 taatcctttt ttacatttga tttaaatcta ctaaatttac catttaatat gtctgtattc
    30121 attatttttc catctatatt ttcttttgat ttactatact tttcttttgc ttcatctttt
    30181 aagatagagt aacctttttt catatctgat ttaaatttac taaacttatc attcaaaaca
    30241 tctgtattta ttattttttc ctcttcaata tttttttctt tagtttgtaa gataagttct
    30301 tttgctatag attttggtga gccaagtgat gctattatct ccatatcact ctttccctct
    30361 ataagaccat ctacgaaata ctcttcataa tctctcaata tatcattaac ttcgctctca
    30421 gaaaaatctt tttttagata atcctttaaa atttcaagaa actcagtttt attcaagttc
    30481 attacctccc tcatcttcac ttattaaaat ctcaacagcc tttacaaatt ctctccactc
    30541 ttccatcaga ttggataaat tctcttttcc tagtgaagtt attctatagt attttctagc
    30601 aggaccttca ttagattcta gtatatatgt ttcgaaatac aaatcttttg taagtcttct
    30661 tagaatagga tatattgttc cttcatttac atctattact ttagagatgt tatgtactat
    30721 ctcatatcca tacatatctt tcttagatat gagagcaagt acacaaattt ctaaaacacc
    30781 ctttctaaat tgtgtgttca tatactccat cctttcttta aatacacagt attgtgtttt
    30841 acaaggtact atgttaaaac ttcttatatc ctcataataa cacagtacta tgttctgcac
    30901 aatagttatt tttaaatatt cttgttgatt aaatccttct ataaatgtat aatactctaa
    30961 tcagatatat tttatgaaag tggtgataaa ttggctaaaa tgaaagatga taaaacaata
    31021 ttaatcctaa ctgctcaatt tggtgctgga catataagtg ctgcaaaagc agtcaaagaa
    31081 tgtattattg aaaaatatag taactataat gttgttatac aaaactttat aaatgcaagt
    31141 attcccatga tgaataagcc tatggtaaaa ctttatgaaa acaatacaaa atatactcct
    31201 ggattatata attactatta ttactttaag aaatcgtttg attcaagaca tgatttatct
    31261 cataaattat atacacctaa actttctgag tatattgctg atataaatcc agatttaatt
    31321 atctctacat ttccactagc tgctgcttgt gtaaataact ttaagataaa aaatcctgat
    31381 ataaatatac ctacacttac agttataaca gatgttgtag atagtatgga atgggttttt
    31441 gaaaatacag atttgtattt tgttccttct cctgaaataa aaaatagatt ttttcagaaa
    31501 ggaataaatc ctgactccat aaaggttact ggagttcctg ttgataagag atttcaaatt
    31561 gaaagtaaag aactatgttg tgataaatat agactattac ttctaggtgg aggaagaggt
    31621 ctatttgata tagatgagga ttttatgcat tggatagatg aatttattga agaacatagt
    31681 aattctatag aaattacaat agttactggt aaaaacaaaa aattatatga caatctaact
    31741 cataaaaaac ctctaaaaaa tattaaagta cttggatttg taaacgacat gtataatcta
    31801 ataagagaat gtgacctaat gctaactaag cctggaggag caactatatt tgaagctatt
    31861 caatcacaaa ctccagtact cgtaaaaatg ccaaaagttg gacaagaaat tgaaaacgct
    31921 aaatttataa tagataaggg tcttggcatg atttatagtg atgacctaga ccttaaaaac
    31981 atattttata aattagtatc aaatgaattt gattctataa taaactttat gaaaaaaaat
    32041 ttaaaagaat ttaaaacagt tattcatcca gagaaaattg ctgattatat atcagaatta
    32101 atagataatc attacagtta aattgtacta ttaaaaaatt tcaaacataa aattaaaggc
    32161 aatcaagtat gctcttacac ttgagaattt actcatagtg tttaataata cttgattgcc
    32221 tttatcatat caaaatcaaa ttgttatatt agtatatatt tttcttgtac tgcatataca
    32281 gttgttgtat ttcttccata cttctcttta attctatttg taaatctgaa gcagtctggt
    32341 aatccttatt ttctatagct tctctcactc tagtgaatag tgattctcta gttaaagtat
    32401 ccttcataat attgaacttt aaatccgata ttgagttcca aactacctca tcaagcgcat
    32461 ccatattttc ttttgtgtgt atcttgacaa tacttcttat tttttgaatc ccagcctcta
    32521 ttattctagt ttttaatttc ttttgccact tatctatagc tccaatctta aatgattcta
    32581 ttatactatc agtaaataca tcaccttgtt ttaaagattt taattttgaa gcatatattt
    32641 ctaagttttt catattttca taaactgtag ctggtggtat tccaaatctt gcatttcttt
    32701 cttccaaact ataatgttca aatacatcat tttcatctct atatgctcta tctttttcta
    32761 aatagaaact ttcttcacct acatttttag ataactcttt ttcaagttct tttgtactaa
    32821 gaccactctt agctgctgct tttataccat ccaacattgt ttgataacat cctgctatta
    32881 ctaaatacgt gttagataga gggcttggtg atcttaactc aaatcttaca gtctttggat
    32941 ttttcatatc tcttattaat ccaacaagca ctgacctatt tcttgatgga atctcataac
    33001 tatgccctaa agatgtaact atgcatacag gagcttcaaa tccaggaact aatctattaa
    33061 agccatcatt agaagctgtt acaattgggt ttaatacttc ataattgtaa agaagtccca
    33121 taagagcccc atatcctagt tcacttagat aatcttcttt taaatctttt ggagcaaata
    33181 gattttttat acttccatcc tttaacttcg cacttactcc tacatgtgta tgtccaccac
    33241 ttcctgctac tccatgaatt ggttttgctt taaatgtaac ttctagtcca tgtgatgtaa
    33301 atacatcctc tataacatct cttactaata attcattgtc tgctgcttgt aatggtgtag
    33361 aaaacttcca agaaacctct aattgctcca tagcatggtt tgttcttcca tctatactaa
    33421 tagaactttg tattcctccg acctctttgt gagccatttc agggtttaca cctagctttt
    33481 gaagtataat taaacttttc tctaaacaag ttcttataat accatgagtt cttttccagt
    33541 attgctcttt taggctttgt gatacatata atttctctaa gtcagcttta tcctcaggag
    33601 tattaaccca gaactcaagt tctgtagctg ctgttaacat tatttcctct atatcatcta
    33661 ctgaatctat tcctatgtta tttattacat gtggatattc tctaaatatt tcatacattg
    33721 actttttaaa gtacttatct gctttttgaa gtactcctct agagcatact tttttattat
    33781 catgtattaa aaatgcaggt atctttaatg ttccaacagg tagacctact tcttcgtcta
    33841 tatattccat attgtaatct acataccaat gacaactttt atctggcatt aaatctactt
    33901 tcgcattatt taatgtagct atattataaa gctcaacact tgaaccatca gtctgtatag
    33961 ctgattccaa aaatttatct atatcctcca aaaataattc tacaggaatc ttttcatctg
    34021 ttgcatttcc tcctaaatca acccccatta aggaaacaaa tcttatattt ttattgcttt
    34081 ttaggatttc tcttaattct tcattagtgt gtttatcttt ctctattacg tataatagac
    34141 tatccatttt tccactctcc cataattttt atgtatattt tattttgtta aatacaagat
    34201 aaatttcatt ttccttacat ttatgaattt ttcttgcaaa ttattcgtgt tttattcgtg
    34261 tttttattta tattattata tatcaagtat aaaaagttcg caatattttt tattggaaaa
    34321 aatattatac atacaatagt ttaacatttt tacaaatatg gggtatacta aaataaaagt
    34381 at
//
LOCUS       DAGSWI010000031        33279 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.031, whole genome shotgun sequence.
ACCESSION   DAGSWI010000031 DAGSWI010000000
VERSION     DAGSWI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.031"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            307..1476
                     /locus_tag="MJF80_001340"
     CDS             307..1476
                     /locus_tag="MJF80_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBZ0280012.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            1525..2376
                     /locus_tag="MJF80_001341"
     CDS             1525..2376
                     /locus_tag="MJF80_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBZ0280013.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            2702..4603
                     /locus_tag="MJF80_001342"
     CDS             2702..4603
                     /locus_tag="MJF80_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBZ0280014.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            4606..5649
                     /gene="ypdE"
                     /locus_tag="MJF80_001343"
     CDS             4606..5649
                     /gene="ypdE"
                     /locus_tag="MJF80_001343"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBZ0280015.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            5664..5969
                     /locus_tag="MJF80_001344"
     CDS             5664..5969
                     /locus_tag="MJF80_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBZ0280016.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            6066..6401
                     /locus_tag="MJF80_001345"
     CDS             6066..6401
                     /locus_tag="MJF80_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0280017.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            6433..7755
                     /locus_tag="MJF80_001346"
     CDS             6433..7755
                     /locus_tag="MJF80_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBZ0280018.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            7765..8835
                     /locus_tag="MJF80_001347"
     CDS             7765..8835
                     /locus_tag="MJF80_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBZ0280019.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            8840..10027
                     /locus_tag="MJF80_001348"
     CDS             8840..10027
                     /locus_tag="MJF80_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBZ0280020.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(10224..11096)
                     /locus_tag="MJF80_001349"
     CDS             complement(10224..11096)
                     /locus_tag="MJF80_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBZ0280021.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(11490..11681)
                     /locus_tag="MJF80_001350"
     CDS             complement(11490..11681)
                     /locus_tag="MJF80_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280022.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            11901..12947
                     /gene="cobT"
                     /locus_tag="MJF80_001351"
     CDS             11901..12947
                     /gene="cobT"
                     /locus_tag="MJF80_001351"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBZ0280023.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            12979..13539
                     /gene="cobU"
                     /locus_tag="MJF80_001352"
     CDS             12979..13539
                     /gene="cobU"
                     /locus_tag="MJF80_001352"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBZ0280024.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            13549..14322
                     /gene="cobS"
                     /locus_tag="MJF80_001353"
     CDS             13549..14322
                     /gene="cobS"
                     /locus_tag="MJF80_001353"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBZ0280025.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            14342..14956
                     /locus_tag="MJF80_001354"
     CDS             14342..14956
                     /locus_tag="MJF80_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBZ0280026.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      15023..15202
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            15332..16831
                     /locus_tag="MJF80_001355"
     CDS             15332..16831
                     /locus_tag="MJF80_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBZ0280027.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            16870..18246
                     /locus_tag="MJF80_001356"
     CDS             16870..18246
                     /locus_tag="MJF80_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBZ0280028.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            18247..19218
                     /locus_tag="MJF80_001357"
     CDS             18247..19218
                     /locus_tag="MJF80_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBZ0280029.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            19250..20320
                     /locus_tag="MJF80_001358"
     CDS             19250..20320
                     /locus_tag="MJF80_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBZ0280030.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            20322..21209
                     /locus_tag="MJF80_001359"
     CDS             20322..21209
                     /locus_tag="MJF80_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBZ0280031.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKSMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            21197..21829
                     /locus_tag="MJF80_001360"
     CDS             21197..21829
                     /locus_tag="MJF80_001360"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBZ0280032.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            21838..23064
                     /locus_tag="MJF80_001361"
     CDS             21838..23064
                     /locus_tag="MJF80_001361"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBZ0280033.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            23061..23669
                     /locus_tag="MJF80_001362"
     CDS             23061..23669
                     /locus_tag="MJF80_001362"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBZ0280034.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRIDNFDMNVVVILDEE"
     gene            23659..24234
                     /locus_tag="MJF80_001363"
     CDS             23659..24234
                     /locus_tag="MJF80_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBZ0280035.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            24227..24979
                     /locus_tag="MJF80_001364"
     CDS             24227..24979
                     /locus_tag="MJF80_001364"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBZ0280036.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            24963..26084
                     /gene="cbiG"
                     /locus_tag="MJF80_001365"
     CDS             24963..26084
                     /gene="cbiG"
                     /locus_tag="MJF80_001365"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBZ0280037.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            26081..26806
                     /gene="cobJ"
                     /locus_tag="MJF80_001366"
     CDS             26081..26806
                     /gene="cobJ"
                     /locus_tag="MJF80_001366"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBZ0280038.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKSGDTPVGVVKDVGREKEEKLVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            26803..27555
                     /gene="cobK"
                     /locus_tag="MJF80_001367"
     CDS             26803..27555
                     /gene="cobK"
                     /locus_tag="MJF80_001367"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBZ0280039.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            27574..29052
                     /locus_tag="MJF80_001368"
     CDS             27574..29052
                     /locus_tag="MJF80_001368"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBZ0280040.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            29082..29987
                     /gene="hemC"
                     /locus_tag="MJF80_001369"
     CDS             29082..29987
                     /gene="hemC"
                     /locus_tag="MJF80_001369"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBZ0280041.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            29977..31476
                     /gene="cobA"
                     /locus_tag="MJF80_001370"
     CDS             29977..31476
                     /gene="cobA"
                     /locus_tag="MJF80_001370"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBZ0280042.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            31493..32458
                     /gene="hemB"
                     /locus_tag="MJF80_001371"
     CDS             31493..32458
                     /gene="hemB"
                     /locus_tag="MJF80_001371"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBZ0280043.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            32611..33189
                     /locus_tag="MJF80_001372"
     CDS             32611..33189
                     /locus_tag="MJF80_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBZ0280044.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        13482 a         3321 c         6126 g        10350 t
ORIGIN      
        1 atataaatat tttatgtatt gacaaaaaat tcaaattaat ttatatttgg attagtggta
       61 attaccactt tggtctgaac aagtttttat cgtaaaataa aaatactaaa gtatatagag
      121 tatgatttta ttaaattttt aaagataaac tataaaatta attaaaatct atagtgtata
      181 ttttatagtg agtttaagta gtactaaaaa atctaagcaa aaaattataa aagagtatat
      241 ttttttatag tttaaagtgg ttataacctc attaccaaat gtaaacgtta caagtatgaa
      301 aggagaatga aaatgtttaa attagaagaa caaaaactaa aagatttagg tgcaataata
      361 acaactaatg aaataaaaca acagcctgaa ttatggttag aaacttatga aatatataaa
      421 tctaacaaag aaaaattaag tagattcata gatacaataa gcaataatca tgggcagttt
      481 agagttatat ttacaggtgc tggaacttca gcttacatag gaaattcaat acttccttac
      541 cttaaaaata aaaatgacat aagaaaatac atatttgaag caatacctac tacggatata
      601 gtttcaaacc catatgatta cttaaaaaaa gatataccaa cattattaat ttcatttgca
      661 agaagtggaa atagtcctga aagtttggct gctttaaatt tagggaataa gattgtagat
      721 aatttttatc atttagctat aacttgtaat ccagaaggag aactggctaa gatgactaaa
      781 aacgacgaaa ataattactt gttattaatg ccatcaaaat caaatgatga aggctttgcc
      841 atgacaggaa gtttttcgtg tatgatgtta tctgctatgt taatattcga tagtttagaa
      901 gatgatgtag aaaagtcata tataaatgca ataattgaaa tgggaagaaa tgttatagat
      961 agaaaagatg aaatacatga gttaataaat aaagattttg atagagttgt atatttaggt
     1021 tcaggaggat taggtggttt gacacaagaa gcacaactaa aacttttaga acttacagca
     1081 ggaaaaatat ctacagtgta tgattctcct atggggttta gacacggacc aaaatcattt
     1141 attgatgaaa atacacttgt atttgaattt gtatctaact gtttatatac aagaaaatat
     1201 gatttagatg ttttagagga gataaaaagg gataaaattg ctaaattcac ttgtgcagtg
     1261 tcagtagaaa atgaaaataa cttctcagga acaaagtttg agtttaaaga aaagtataat
     1321 aaattaccag atgtgtatct tgcaatgcca tacatattgt ttgcacaaac tatagcattg
     1381 tttgtatcgg taaaggttgg aaataagcca gatactccat cagctacagg aacagtaaat
     1441 cgagtagtaa aaggtgtaac aatatatgaa tattaaaaga taagattaaa atattataac
     1501 taaataattt gggagggtat caatgtggca ttagtaacaa caacatcaat gcttaaaaag
     1561 gcacaagatg aagggtatgc agtaggagca tttaatgtag aaaatatgga aatggtaatt
     1621 gctgttatag aagctgctga ggagttaaat tctccagtaa tattacaaac tactccatct
     1681 actgtaaaat atgcaggtct tgattattat ctagcaaatg caaaggttgc tgcagaaaga
     1741 gtaaatatac ctgtagcaat gcatctggac catggttcaa gttttgacct tgcaatgaaa
     1801 gctttaagaa gtggatatac atcaataatg atagatggtt ctcatgaaag ctttgaaaat
     1861 aatatatcta tatctaaatc agtagtaaaa gcatgtagtc cttctcttat accagtggaa
     1921 gcagaacttg ggaaagttgg tggaaaagaa gatgatttag atggcgggaa tagtaatgat
     1981 tatacaaatc caaaagaggc aaaagagttt gtcgatagaa caggaatatc ttcattggct
     2041 attgctatag gtactgcaca tggattgtat aagggtgaac ctaaaataga tttagatagg
     2101 ttatcagaaa ttaaaaatgt agtttcaata cctttagtat tgcatggagg ttcaggaata
     2161 catgatgata ttataaaaga agctataaaa agaggtattg caaaagttaa ctatgcaact
     2221 gaattaagaa ttgcatatag taaaggggtt aaaaaagttt tagatgaaga ttctgaggtt
     2281 atagacccta aaaagtatgg attagcaggg cttcaatcag taaaggattt tgtgaaagaa
     2341 aagattaaag tgtgtgggtc tgtaaataga gtataaaaat aaaatatcct aaatttattt
     2401 tagataagtt taggatattt gttatatgaa aaatttatat taattatatg taaatggtgt
     2461 atttttttca tttaattgat gaaaaatatc agcttttatt ggtaaagtat tacatatata
     2521 attaaaatat actaaaaatt gaactatata ttttattatt ataaaatatt tatattaaat
     2581 tgtggaatta aaatatacta ggatataaaa ttcttaaata aaaagaaaag attgttttta
     2641 taaaaaatat tttttatacc actttaaaaa acacaataat aacactgagg gaggtgggga
     2701 tatgagagaa cgcattattc ttgaaacatt aaaaaatagt agtggttatc taaacataga
     2761 ttattttgca gataagttgg gggtatctac tcgaactatc agaaatgaaa taaaaaagat
     2821 agaaactata aaagaatgta atggttttaa attagaatat aagtctaaat taggatatat
     2881 tctaaatatt aaagaccaag ataaattcga gcattatcta agaaacttac cctcagattt
     2941 agttgaaaat ccagaacaac ggttggagtc tataatagta gagcttttag taaatgaagg
     3001 ttataaaact atagaacagt taagtaaaaa atttctagta agttcatccc aaataaaaaa
     3061 tgatttaaaa aagatagatg aaaagataaa ggatacagaa ttaaaactag agcgaaaggc
     3121 tcattatgga attaaaatag aaggttctat aaaagaaatt caaagtatat tggtggatag
     3181 ttattttaga gggaatagaa atataacaga gtacaggaat aagtttatag ataatttaaa
     3241 attagctaat attagaagta caataaaaaa tgtacttaat gaacatgatt tagaagcaaa
     3301 tttaacagaa cttgaggaaa tattagcaca aattattata ttgtatataa gagttagtat
     3361 gagagtagtg ggaaatgcta gcgatttaaa attatataca gaagatttaa ttatagataa
     3421 attattagat aaaattttta gagataaaaa atataacctt aattatgatg aaaagtatta
     3481 tttaaaggag cttataaaac taaagactaa agataagaaa gccactataa agaatattga
     3541 taagaataaa cttcaagata ttatgttcga attttttaga gagatagata aaaaatataa
     3601 ctctaatttt ttagaagata aagagttttt taacttattt tacttacata ttgcatgttt
     3661 aatagaacgt ataaagaaga accacaagat aataaatcct ttttcagtta aaataagtca
     3721 acagtatcca actgttttta acttatctat tcaattttca aaaatcatag aaaatgagta
     3781 tcatataaaa ataagtcaag atgaaatagg gtttatagct actcacattg ctgttccttt
     3841 tgaaaagaga gaagaagcta attttaataa aaaatataaa atagcaataa tatgctcttc
     3901 tggtggaggt agtgcatttt tgataaattt aagacttaaa gaaatttttc caaatgctga
     3961 gataagaaat ttttcattac tagaagaaaa agctgtaata gaatttgcac cagatttgat
     4021 tttttcaata acaaatcttt tatttgaaat aaatgctcca gttatactta taaatgagat
     4081 tttagacgaa ttggattatt tagatattaa agaaagtgtt agatttgcag accatatagg
     4141 aaatatatca agcccaaaac aatatatatt aggtctattt gataagaatc actttaggtg
     4201 tataaaagat aaagctaagt ataaggatat ccttgatagt atgtcaaaat gtatagttga
     4261 cgagggagct tgttctccaa cctatcccaa agatgtatgg gaaagagaaa gctatttgag
     4321 tactatttat acaaatgggg tggctatacc tcatccaatc gagatgacag gaaacaaaaa
     4381 tataatatca gtagctttga tacaaactga tattatttat gaaaataggg tgcctaaaat
     4441 tattttcatg atttcattaa taaagggaaa tttagaactt cataagcaaa tatctaaata
     4501 tttaagtaag attatggcaa ataaggatat ggtagacatg ctaaataagt cacaatctta
     4561 tgaagagttt atgtataagt taaaaatcta tctgggaggt tagacatgaa tatagagttt
     4621 tttaagaggt tgtcagaagc agatggtata gcatctaatg aagaagaggt tagacatgtt
     4681 ttattggaag aactaaaagc ttatagtgat aaaattatct gtgatgggct agggagtatc
     4741 atcttttcta aaataaaaga tgagagtgca ccaaacgtaa tgatttgtgc tcatatggat
     4801 gaagttggat ttatggtgag aagtatagac aaacttggta tgattcactt aataacaata
     4861 ggaggagtaa aacctcttgc acagtttgta caaaaagtta gaattacaac aaaagaaggg
     4921 aagaaaatcc ccgctgtcat aaatgctaca tataataatg gtaaggctga aaatatatat
     4981 gcagatattg gagcatatac agaagaagat gtatataacc tagggattaa tgttggggat
     5041 atggtaacat atacaacaag ctttgaggaa tttactttac cagaccgatt agtgggaaag
     5101 gcttttgatg ataggatagg atgttttgtg atgggagaag ttttaaaaga acttaggaaa
     5161 gaaaatctaa attgtaatat tcactttgcg gctacaagta gtgaagaggt tggaataagg
     5221 ggagcaaaga catcaacaca gctaataaat ccagatatag tatttgtaat agatgtagca
     5281 tgtgctaaaa atgagtttgt gagagactat acaaatcaga aacaaattga taaaggtgta
     5341 atgctaatgc atagagatag gactctagtc cctagtagaa aaatgataga ttatgtaatg
     5401 gaaattgctg ataaaaataa tataccactt caacatgata tgtttgaatc aggtgggact
     5461 gatggaggag aagcacatct cgtaaatgaa gggaaacctt gtgtagtaac ttgtgtacca
     5521 gttagatatg gtcattgtgc attttcaata gtaagtaata aagatttgga gaacattata
     5581 agattgtata cacagttaat tttaaatttt gatgaagaac aatataaaca tataagaaat
     5641 tttatctaaa ggagaataag aaaatgtcaa cagaagtaca aataatggaa cttatatcaa
     5701 ctgcaggaga gagtaaatcg aaagcttttg aggctttaaa aaaagtgaag tcaaaagatt
     5761 ttaaaggagc aaaattgtta atagaggagg ctcgtaaaat agatattgaa gctcacagga
     5821 tacaaacaga attaataact caagatttaa atgaagatgt tgaaagtcca gcaatgagtc
     5881 ttttaatggt acatgctcaa gaccactata tgacttctca gctagcaaaa gacttgatag
     5941 aggctttgat atattgtctc gataagtaat tgtaatgtag aaatagatta tttatgtaaa
     6001 aattctttta aaagtttaga tgcttttaag agataataaa aatacaaact ttaggaggaa
     6061 ttattatgaa aatattatta tgttgtgcag gtggattttc tactaatatg ttaatgcaaa
     6121 atatgaaaaa ggtggtaaaa gagagtgcta aactcaatga aaaagatttt gagtttacag
     6181 ctataccagc agattcaata gaaagtgaag ttgatgattg ggatatagta cttataggtc
     6241 ctcaaatagc tcacaaaata gacttcttaa aaggaatatt agagccaaaa aataagcctt
     6301 ttgcagtaat agataaagat gtatatggaa aaatggatgg agctacagtt atgaagttgg
     6361 cattaataac ttataagaaa catcaattag ctcaaggata ataaaaataa tgagggaaaa
     6421 gggagattag atatgttttc aaaatttgaa gcatttatga atagagtatt tatgccttta
     6481 gctcataagg ttgacaatca acgtcacctt ggttctataa aggctggtat ggttgctatg
     6541 acaccattca caatattagg aagtatattt gcaatacttc cagcacttcc aaatatgctt
     6601 ggagaaaata atccaataag caaattcata cttaacaatt cagcaatatt ggatttacca
     6661 gtaagtcttt ccataggttt aataggatta tatgcttgta tgtgtattgc ttttaatctg
     6721 gggaactact ataaattata tataccaggt tgtgttgcac tatcaacttt ttctttttta
     6781 ttcctagtag cctcatttac accagatgga gctttagaaa taggaaattt aggagctaaa
     6841 gggctattta caggtatgat agcagggctt ggttctgtgg agttatataa tttttgtaat
     6901 aagaagaata tcacaataaa aatgccagaa aatgtacctg attttgtatc taggtctttt
     6961 gaattaatac ctactactat aatagtttgt ggaactttta tagttgctcg ttttatttca
     7021 ttaaaagtat ttggagaatt acctccacag atacttactc aatttttatc tccattagta
     7081 ggaagtatgg ataacccatg gtcagttctt gcacttaact ttgctatatg tactatattc
     7141 ttttttggaa tacattcatc agtatttagt cctataacta gacctataat gattacattt
     7201 atagctgaaa acatagcagc tatgcaagct ggagaaacaa tacctcattt ctttacagca
     7261 ggagcttcaa gtgcattctt tggatttaca ggatgtggaa ttagtatagg ttgtgtaatt
     7321 gcatgtatgt tatcaaaaaa tgcttcctat aagaagattg gacgtgtatc attattccct
     7381 gcattatttg ggataaatga accaatatta tttggagcac cgataatatt aaatccaata
     7441 atgtttatac cacatgtatt tggtggagct ataataggta cactaccaat gttttttatg
     7501 cactggggat ggttagcaaa gccagtattt gaccctccat atgtaggggt attcttagaa
     7561 ggatttttaa ctaatggaga ttggcattct atattagcaa atgcattgca attagtatta
     7621 gcagttttat tatattggcc attctttaaa ataatggaac gtcaacaaaa taataaaaaa
     7681 caaaaagaag aaactaagaa tatattctct aaagaagaag aagacttatt ggatgattta
     7741 ggattggatt tctaatattt tgttatgaat aatacaatgg agagattagt atccttaatc
     7801 tataaaagag gtgttgacgc tcttttgatt aaagcaaaaa atactaaaag atatatagga
     7861 gctttatcag gtagtggtgt atatgtgctt gttactaagg ataaaaatta tcaaattcta
     7921 gatggcagat atattgatga agcagataaa aaaacgactg gtttcataaa aaggattgta
     7981 tctcaaggtt cttatatacc aactataatt gagttattaa aagaattaaa tataaaaaag
     8041 ttagcaatag aaaaccaagc tatgagtata caagagtatc tttcactaaa aaatgaaggt
     8101 tttgaaatca atttaataac aaatgaatta tggaagctaa gggctataaa atctaaagaa
     8161 gaaattgaac ttattaaaaa ggcttgtgaa attacagatg aagtatttga tgaggttatt
     8221 tctgaaatta aaaaagatat gactgagcta gaagtatcag ctctaattca gtatcatgca
     8281 ttaaaaaaag gtgcaagtgg aatgtctttt gaaacaatag tggtatcagg agaaagaggt
     8341 gcaatgccac atggaagacc tacaaacaaa aaattgaaag tgaatgaagc tataacaata
     8401 gattttggtg ttgtttatca agggtatcaa tcagacatga ctcgtacaat atctataggt
     8461 aaacctccta aaattattaa agagatatat gatgtagtat tggaagcaca attatctgca
     8521 atagagtcaa taaaggaagg aactagggca agtgatgttg acaaagtggc tagaaaaatt
     8581 atagataaac atggatttgg cgaatatttt aatcatggac tgggtcatgg tataggtctt
     8641 ggagatggtg aagttcccac tttaaatcca aatagtgaag atatattagt ggaaggaatg
     8701 gtaatgtctt gtgagccagg tatttatatt ccaaatgtag ggggagttag aatagaagat
     8761 gatattgtta taatagatgg aaaaggtgtt ccactcaata agacatccaa agagtttata
     8821 atattaggag agtaaaatta tgaaatataa ttttaataaa gtattaaatc gtaaagaagg
     8881 aaattgtaga aaatggagca accatgtgat taaagaaaaa tttggcttaa atgaagatgc
     8941 aattcctatg gatttagctg atatcgattt tgagtgtgct cctgctataa aagaatctat
     9001 cttaaaaaga gcatcagtag gagattatag ttatactttt ataggagatg acttctatga
     9061 tgctattata aattggaata aaagaagatt tgatgtatat atagaaaagg attggataaa
     9121 attaactttt ggtactgttt caacccttca ttatatagtc caagcatata ctaaagaagg
     9181 agagggtgta ttgataaata cacctgctta tgaccctttt gcagaggctg tagtaaataa
     9241 taatagaaag ttgtgttgta gctcattaaa attagataac aatagatatt atttagattt
     9301 agaggatatg gaaaatcaaa tgaaatacga gaatataaga ttgcttatat tctgtagtcc
     9361 acaaaatcca tcaggtcgta tatggacaaa agaagaacta tatcaagtgt cagagttatg
     9421 tttaaagtat aatgtaattt tggtaagtga tgaaattcat agagatataa tatttaaaga
     9481 ttatagattt gtatctttgt ggaatgctca tccagaaatt tataaaaata gtataatctg
     9541 tgtatctcct aataaaggct tcaacttagg tggattaaaa acatcatatg tattaataag
     9601 agatgtaaaa attagggaga ctttattaga aaggctaaaa agtaattcaa taacttctcc
     9661 aaatgtattt gcaataccag caatagttgc agcatataat gaaagcgaag aatggctaga
     9721 tgctatgact agttatgtag aggagaattt tgaaatagtt tatgatttct ttgaaactaa
     9781 tataccaaaa gctaaagtga tgaagtcaga ctcttctttt ttagcctgga tagatgtaag
     9841 agaagtattt agaaatgaag aagaatctaa agaatttttt agacatgcaa acttaactat
     9901 ggtagttgga agttattttg tgaaagatgg agatggattt attcgtatta atgtggggtg
     9961 tcctagagaa acattaaatg aggctctaaa tagaatcaaa aaaacgtata taagtatgta
    10021 ttgttaaatc ataaaaaaag tatctatggt ttatataaaa acacattatt tagttatcaa
    10081 atattactgg aattttgtat tccagtaata ttttttgatt aaggaatatt attgaggaat
    10141 attaaataat attatttcat acccttatat taacttatat aaaaatattt tattattttg
    10201 ggaacgggtt agctaacctt tatctagtgt atatttatat tgtaagaagc agtaaaaact
    10261 tttcctttac taagtttatt aataaacttc ttatctttat aaattttgtt agaatttata
    10321 ctatcagcaa gaccatacca aggttctata cataaaaaag gagcagtaga attagtttca
    10381 ctataataag gagtccaaat accaactaga gggaaatctt ccatagatat agttacatat
    10441 ttactaccat cattattaca tatagacact tcatctatgt tggtgtatat caaagcatca
    10501 ttcttgaata cttctggatt taactgtaag ttttttaatt ttccaatagg tattatatca
    10561 tctgtaaaag aaccatttaa attgattttt tctacatcat ttttactttt aaactcaagg
    10621 tagtgtttag aaaaatcatt ttgttcgtaa aaaggaaggt tgaatgcagg atgtccacct
    10681 atagaaaaga atatatcttt agaatcagta ttttttacaa tccattctac ctcaatacta
    10741 ttatcattta atgtatagtt tatgattagc tcaaaagaat atggatactt ctttaaagta
    10801 gattcattat ctgttaattt ataagttaca aatgtatctc ctttatctac aatctcaaag
    10861 tccatatctc tagcaaaccc atgttgagtc atgctatata aattttcttc tattattgtt
    10921 tcattatcaa aaagtttacc tactataggg aacaatatag gtgattgtct accccaatat
    10981 tttttattac catcccaaag aatgtttttg tcgtattttt tagaaaatat tttagttagt
    11041 tcagcaccaa aactgcttga ttcgataatt aaattttcat tttgtaatat attcatttaa
    11101 aatctccttt attatatttt atgagtatat tatactataa agtataaaga ttattaaatt
    11161 ttatttgata ttatatttga aaggtagatt ttagtatatt ttgaaaattt tgttttgaaa
    11221 atttgggatt atttaagaaa ttttgtatta taaatagaag gaaatagata aattgtatag
    11281 aataaaatat attaagataa gttaataatg ttaaatggga agtgagttta tggaaaaacc
    11341 aaatacaaaa gttgttaact ttcaagagta taaagacaga aaaaaccaaa gagaagtaaa
    11401 tagagacgaa cttttaaagc ttataaaaaa aattgtgact tcaaaatagg gagttgttaa
    11461 actccctatt ttaatgggtt tcataataat taagacattc taagtccatc tatataatct
    11521 ttgaattgtg agaaatctga ttcactactt aattctatgg tttctgaacc ttccgtataa
    11581 aaattcttaa gatagatagt ataattatta tcaacacaat catgataaat acatctatat
    11641 ccatcttcat acaaactttt taacttagaa aaatcatcca caatatcatc tccttaaagt
    11701 tcttttaaat atatttttac aaaaagcatc taattgtatg caaattgaca atgtaaaata
    11761 aaggaaatga acataaaatg tagaataatt tttatatgta tgtaaaaata aaaagaaaaa
    11821 gttgaaattt ttagaaaata agagtatttt ttatctaaaa agagagttta aaaaataaaa
    11881 aaatgttggg aggataaaaa atgagtttat tagaaagtat atcaaaaaac atatattccc
    11941 tagataactc ttctattgaa aaaacaaagc agagattgga taggcttata catccaactg
    12001 gaagcttagg aaagatagaa gatatttgca tgcaattagc aggaatattt ggcaatgaaa
    12061 attttgacac aagtaaaaag gttataattg cttttgcggg agaccatggt gtatatgaag
    12121 aaggcgtagc acctgaccct caaaatataa caaaattaca gtttccaaat ttttcgaaag
    12181 gtctatgtgg agttggagta ataagtaaat ttgtaggggc agatgttgta gctgtagatg
    12241 taggaataaa ctgtgatgaa aaactggatg gggtattaga ctacaagata agaaaaggga
    12301 catcaaatat ggcaaaaggt ccagctatga gtaaacaaga agcaattaga tgtttagaga
    12361 taggtattga aatagcagag cagtgtatag aaaaagatta taaggttatt ggtattgggg
    12421 agatgggtat agcaaatact actccaagta cagcaataat atctgtaatt tcagggtgtg
    12481 acccgttaga agttactggt ataggtgcag gtcttaagaa agaaagacta aagcataagg
    12541 ctgaagtaat tagaaaggct attgaaatta ataatccaaa tccaactgat ggagtagata
    12601 ttttatctaa agtaggagga tttgaaatag gctcaatggc aggtgttata ttaggttgta
    12661 gtgctaatag gattccagta gtaattgatg ggtttatttc ttatgcagca gcattaattg
    12721 catataagat aaatccaaaa actagagaat atatgatagc atctcattta tcagcagaat
    12781 caggaactaa aagagcatta gacatattaa aattagaccc tctattaaat atggatatga
    12841 gattaggtga gggaagtgga gctgctttag catttgatat aatagaagct tcaaactata
    12901 cttacaaaaa tatggcaact tttgatgaaa ttgacatggg aagataaata taaaaattta
    12961 atatgtagga gtgttttgat gagtaaaatt attctagtaa caggaggagc taggtcaggg
    13021 aagagtaact ttgcagaaaa actatgtttt gacagaaata ataatacagc ttatatagca
    13081 acctctattc catttgatga tgaaatgaaa gatagagtaa aaaaacatca agagaataga
    13141 cctagaaact ggaatactta tgaaatatat gaagatatat attccataat taaggagata
    13201 tataaagaac atgaaactgt tatactagat tgtgtaacat tattagtaaa taatctaatg
    13261 tttgaacaca atctaaatat agaagaatct agtcaagaag aaataaacaa attagaacaa
    13321 tacataaaag aacaagttgt caaacttatt gaagaaatag aaaaaacaaa tctatacttt
    13381 gtatttgtaa cgaatgagtt aggtatggga attgttcctg ggaataaact tagtagaatt
    13441 tatgctgata ttgtaggtcg tataaatcaa tatatagctt cgaaaagtaa tgaagtttat
    13501 tttgtagtta gtggcatagc tatgaagata aaggagtagt atgcaattat gaaaagattt
    13561 attttaatac tacagttttt gacaaggata cctataaaat taaatgtagg atttgatgat
    13621 gaattttata agtcaatagt atattttcca cttgtaggtt ttgtcatagg tatattatcc
    13681 tatttaatag gatggatttc tatgctttta tttgaaccat ttatagcttc tataataata
    13741 actctagctg gtgtcttaat tactggagga cttcatatag atggattagg agatacattt
    13801 gatgcaatat atagttatag ggataaagaa aagatgcttg aaataatgaa agattcaaga
    13861 ttaggtacaa attcacttct agcaattatg tttgtacttt tattaaaggt tggattcgtt
    13921 tacgatataa taagtaataa ttcattgtgg gttataattt ttatgccaat gattgcaagg
    13981 ttaggagtaa tgttattgac atataaaact gtgacaccaa gagaaaaagg aatgggaaat
    14041 ttatttatag gaaaattaac tacaagtatg ttaataactg ctataatata cacattattg
    14101 atagttgctc ttattactaa atttatattt ttattaccta atatagtgtt gattaaagta
    14161 ttaggttcga ttatagtggt ttttgtattt ataatattat tcaaaaaaca tatttataaa
    14221 aaaattgacg gagtaacagg agatatatta ggctgtggaa tagaactttc agagcttgta
    14281 tatttaattt acatatattt gttaattttt atgttttttt aaagtaaaaa gtggtgataa
    14341 tatgataaga ttaatattaa taagacatgc actgacaaat gataataaaa aaggaagatt
    14401 atcaggccat ataaatagtt gtattagtga agagggcaaa ctacaaataa ataaaattac
    14461 cagatatctg agtaatgaaa acatagataa ggtttataca acaccatcta cacgtactaa
    14521 agatacagta gaaaaaatat caaaattgaa attattagaa atagaagaaa aagaagcttt
    14581 aagagaaatt agttttggag actttgaagg aagaactttt gaagaaatta aaatcaaata
    14641 tccaaatgag tttgaaaaaa tgataaaaga aggtaacaac tatagatatc caaatggaga
    14701 aagtttgata gactcttata aaagagtggc gaaagaaatt gataatataa ttttagaaaa
    14761 tgatagtaat ttagatacaa agacaatact tatatgttct catgcaggaa caataagaaa
    14821 tataataaca catttgattt ctggtagtta taagtatcac tggaacttta aaattgataa
    14881 tgcatctatt acagttttag aaattgatgg tggatttgct gtaatagata aaatgaattt
    14941 tacagacttc atataattta atcaaaacca aacattacaa tctgtgtttg taatatatct
    15001 gtaaaattag caatgaaaat agaatattag attaaaatta ggttcttaat tagatgttaa
    15061 aagggaaaaa ggttagatgc ctttgcagcc cccgctactg tgaaaccaac gaaactataa
    15121 ataccactgt cagtttgatg ggaaggttat agaagtagga tgaagttaag ccaggatacc
    15181 tgcctaattt aatttaaaac aagaggtctt cggggtgaaa gatttttaat taatatatat
    15241 aaaagtccta tctttagaag tattaggact ttttttaatt tgttttttag taaattttaa
    15301 caaggaggga tggaaagtgg ggaaaaaaat aatgctacaa ggaacagctt caaatgtagg
    15361 taaaagtgtc ttagtagcag gtttgtgtag aatatttaag caagatggtt atactgttgc
    15421 tccttttaag tcacaaaata tggctttaaa ttcttttata actaaagaag gattagaaat
    15481 gggaagagca caagtatttc aagcagaggc tgcgaaaata gagccaatag ctgatatgaa
    15541 tccagtttta ttaaaacctt ctggaaataa taaaagtcaa gttattgtaa gaggtaaagt
    15601 tgtaggagaa atgccttctt ctaagtatca tgattataaa ttggagttag tagatgtgtt
    15661 gaaggaaaca tttaatagct taaattcaag gtatgatatt gtagttatgg aaggggcagg
    15721 aagtaatgca gagataaact taaaagatag agatatctct aatatgggta tggcagaaat
    15781 agctgatgca cctgtaataa tagtcggaga tattgataga ggaggcgtat ttgcctcatt
    15841 agcaggaaca atgcttttat taaatgaaga tgagaaaaga agagtaaaag gtgtaataat
    15901 caataaattc agaggaaaaa aagagctttt acaagatggt gtaaagatgc tagaagacat
    15961 cataaaagta cctgttatag gagtagtacc ttatacagat ataaaaatag aagatgaaga
    16021 cagtgtaact acgagattta agaaaaaaat gaataaaagt gatatacaca tagaaataat
    16081 tagaactcca catatgtcaa attttacaga ttttaatata tttgagacac aagaagatgt
    16141 aagtattaga tatgtagact atggagaatc tttaggaaat ccagatatat taataatacc
    16201 tggaactaaa agtactatag acgatttaac atatataaga aaaagtggtc ttgaatcgca
    16261 aatcaaaaat ttacatagtc aaggtaaatt gatatttggg atatgtggtg gataccaaat
    16321 gttaggtaaa aaattaaaag acccttatca tgttgaaagt gaaattgaag aagttgatgg
    16381 aataggtctt ttagatacag aaacaacgtt tgagaaagaa aagactacaa cacaagtaga
    16441 agctactatt gttgaatgta tcagtgaata catgaataac cttaagggaa aaaaagtaaa
    16501 aggttatgaa attcatatgg gaattacaaa gcgaggtaat gatgtaagaa gtttagattt
    16561 aataactaaa aaacttgata aagaagtgaa ttatactgag ggaagtgtaa ataaagaggg
    16621 gaatgtaatt gggacttatc ttcatggaat atttgatgaa ataagcttta caagagcaat
    16681 tttaaacaat ataagaaaga aaaaaggttt aggagaatta gaaagcaaag taaactcttt
    16741 taatgaattt aaggataaag agtatgataa gttggctgat tttttaagag aacatttaga
    16801 tatagagaaa atatatgaga ttatgaaata atgatgactt ttatttataa tttaggtagg
    16861 aggtgtatta tgaaaaaaat tttaattgcg ggtactaata gtggagtagg taaaacaaca
    16921 atatcattag gaataatgca agctttggtt aagagaaata tgaaagtaca gccatataaa
    16981 gtagggccag actatataga cccttcatat cacactttta taactggaag acattctaga
    17041 aatttagatt cttatatgtt agatgatgaa aaaataaagt atatagtaaa taagtcctca
    17101 aaagatgcag atatttcagt aatagaaggt gttatgggct tatatgatgg ctttggaata
    17161 gatttagata attgtacaag ttcacatact tcaaaggtat taaagtcacc tgttatatta
    17221 gttataaatg gtaaatctat ggcagcatct agtgctgcta tggtacttgg atataaggaa
    17281 ctagataaag atgtaaatat aaagggtgtt atagtaaata atgtaaagac taatagccat
    17341 tatcaaataa ttaagagttc tatagaaaaa tattgtaatg ttgaagtatt ggggtatttt
    17401 cctccaaata acaaattctc tttggaatct cgacatctag gattagttcc tagtgtagaa
    17461 atgaaatcac taagggaaaa attttatacc ttagcagatg aaattgaaca ctatatagat
    17521 attgacagaa taattgaaat atcagaaagc gatgaatttg agagtgattt tgattttcaa
    17581 actttaatag aaaataataa aataaaagaa catgttaata ataaatcaat agctatagca
    17641 tatgataaag cattcaattt ttattataga gaaaatatag agcttttaga ggaacttggt
    17701 ttagaaataa actattttag ccctcttgaa gatgtatcag tgccaaatgc agattatatt
    17761 tatattggag gaggttttcc agaaatattt ggaaaagaat tagaagcaaa tgagtcaatg
    17821 agaacttcca tattagaagc acataacaat aatataccta tttatgcaga atgtggagga
    17881 ctgatgtacc taggtgagaa actattgaat caagaagaac aagaatttaa tatggttgga
    17941 atatttagtg gaattagtaa gatgacatct tctttaaaga gatttgggta ctgtattggt
    18001 gtagccaagg aaaatactct tttatctaaa aaaggggaat ctattaaagg gcatgagttt
    18061 catcattcaa tatttgaaag tgatgaaaaa tgtgtatatt caatgagaaa agaaagagat
    18121 ggaaatatag tagaagagtg ggatggtggt tatagtaaag gaaacacttt ggctacatat
    18181 cttcatactc atttttataa taacttaaat tgtatagaaa attttttgaa aaaaggaaat
    18241 gaataaatga gtattttatc tatttatata ggatatgtga cagacttaat tgtaggagac
    18301 ccatattctt ttcctcatcc agtgagattt attggtaaac ttataaattt tactcaaggt
    18361 ataataagaa aggtctctaa aaatgatagg cagttaaaat tggggggatt tatattatgg
    18421 tttataaccg ttggaataac atatttaaca acttatgcta tagtgaagtt atttagtttt
    18481 aatatattat tgagtattgt agtgaatagt tttataattt atacaacact agccacaaaa
    18541 tgtttaaaag acgaagcatt aaaaatttat aatgtattaa aaactgaaga tatagaaaaa
    18601 tctaggattc agctatctta tatagtaggt agggatacta ctaatttaag tgaacctgaa
    18661 ataataagag caactgtaga aactgttgca gaaaatactg tagatggtat aattgcgcct
    18721 atgttttatg catttattgg aggagcacca cttgctatgg cctataaagc tatcaataca
    18781 ttggactcaa cagttggata taaaaataaa aagtataaag atattggttt tgcatctgct
    18841 aagattgatg atatagccaa ttatatacca gctagaatat ctgtcattct tatgacaatt
    18901 ggaagtttct ttttaaatta taactatagg aactgtttta aaatctctat tagagataga
    18961 aagaatcata aaagtccaaa ttgtgctttt tcagaaggtg cagtatctgg tgctctagga
    19021 atacaactag gaggtaccaa cgtttatttt ggggaaaaag tatataagcc aacaataggg
    19081 gataagctta gggagataga taaagaagat ataattaaaa ctaataaaat tatgtatgca
    19141 tcttcatttg tatctatgtt ggtatttaca ctaatatatg tgttatgtag gatgatttta
    19201 attaacttaa ttatataaat tattgtttgt taacttaagg gggacataga tgaaggattt
    19261 agggcatggt gccaatgtag atgaaatggc aagattatat gggaaaaacc caaaagaaat
    19321 aattgatttt agttcaaaca taaatccaaa tgtattacca aatttagaga gatacatttt
    19381 aaaaggatta gaagagtgta ggaattatcc agatataaat tatacaaact tgagagaaaa
    19441 tatatcaaaa tatatagata taaatccaga ctttataata cctggcaatg gtgcaacaga
    19501 agtgatttat ttgcttatga agagtattaa aaaaaaattg gctataataa atccgacttt
    19561 ttcagagtat agaagaagtg ctgagttaaa taatttagat attatagatt tagagttaga
    19621 tttagaaaat aactttaaac ttaatataga tataataaaa gaaaatataa aaagatttga
    19681 tagtttattt atatgtaatc ctaataatcc gagtggtaat gtccaagatt taaaggaact
    19741 tgttcattta ctagataaac ataataaggt attaataatt gatgaaactt tcatggaatt
    19801 tgttgaagat gaaagtaaat atagtttggt taaatatata gaatcaaata aaaatatatt
    19861 tataattaaa gctgtgacta agttttttgg tatgcctggt ttaaggctag gatatggtct
    19921 tacaagcaac actgaaatta tgaacaaaat ttatgaacac aaagagccat ggactatcaa
    19981 ttcttttgca gatatactat ccaattttat ttttgaagat aaagagtata ttaaaaatag
    20041 taaagaatat tacatagaag aaagaaaata tatgttacaa gagttgagaa acataagaaa
    20101 tataaaggtc tatgatacag atgctaattt tatcttgata agaatatata aaaaaacaac
    20161 taaagaattg aaaaaagatt tgtttaagca aggaaacata ttagttagag atgcatctaa
    20221 ttttatagga ttagatgata gttttataag agtggctata aaatctcatg aagataacaa
    20281 aattcttata gaaaatatga aaaatttatt gggtgattaa aatgaaatct tatggaatat
    20341 gcccagcatc ttgtggagaa tttgtacaag gtataattga tgatgaagaa tatttatgtt
    20401 catatgcaat agatatgtat tctaaagtgt atattgaaga aaaattagta gatataaatt
    20461 tagggagata taagtcaagg ttagcaattg aaaaggtatt tgaaaaattt aatttaccta
    20521 aaaaatatac taaaaatatt tctttaaata taaatagtaa aatacctgtt ggaaaaggaa
    20581 tggcaagttc aactgctgat ataggagcta ctataaaggc aacattatca ttgatagata
    20641 aagatttaag tagtgaggag atttctaaat tagcagcaga aatagaacca acagattcta
    20701 tatttataga taaaaatagt atatttaatc ctctaaatgg aactgttata aaatacctag
    20761 gaaatctgac caatgcgaaa gttgtaatac ttgaaccaaa caaagtacta gatactatga
    20821 aaattagatt gaggcaagat tataataaat taaaagtaga aaataaagaa gtaataaaaa
    20881 aatcatttgc tcttttagaa gaaggattga aaaaaaataa tttatctcta gttggagagg
    20941 cttgtacttt gagtagctta gcaaatgaga atatagaaaa aaaagagtat ttaaatgaaa
    21001 taataaaaat atcaaaaaaa tatggagcat atggagtaaa tattgctcac agtggaactg
    21061 tagtaggtat tttaattgat aagtctatga atgataaaaa gatgatagat gccttatgtg
    21121 aatctaacat aaattctgtc tataataaaa tttatacaca aaacataatt aatggaggaa
    21181 ttaaggggga aatagaatgg aatacataaa gaatcctatg aaaatagaag aaaaaagttt
    21241 tgaattgata caagaaataa tagacgaaat tagaccagat tataaattta agaataatat
    21301 cgaagaaaaa ataataaaaa gagctataca tactactgct gattttgatt acttagatat
    21361 attaaaaata tcagaagaag cagttgatag tataatagat gcattaaaaa ataatgcaag
    21421 tatttatact gatacaaata tggctcttag tggtataaat aaaagaaagt tagaatcatt
    21481 aggatgtaag tataaatgtt tagtggcaga tgatgaaact gtaaaattag ctaaagaaaa
    21541 aggaattact cgttctatgg cagcagtaga aattgcagct aaagaagaag gaagaaaaat
    21601 attcgtttta ggtaatgctc caacagcttt atataaagtt atggagatga aatcagaagg
    21661 tagattagat ttagatgcag ttataggagt tcctgtaggt tttgtaggag cacaagaatc
    21721 aaaagatgaa gttgaaaaaa cagatatacc atatataatt tctaaaggta gaaaaggtgg
    21781 cagtaattta gcagcagcaa tagtaaatgc tatattatat actatgtagg tacctatatg
    21841 gaagaatatg tatatataga tggaaaaaaa tatagaagag gatatactac aggttcatgt
    21901 gcaacaggtg catctaaggc tgctgtatat atgttgatta caaaaaatag aatcaatact
    21961 ataaatatag atactcctaa ggggatacca ttactactaa aagttgataa tataaatatt
    22021 tctgatacat ttgtggaatg ttccataaaa aaagatggtg gagatgatat agatgcaact
    22081 cataccatgg atatttatgc aagagctgaa attgtagcta aaaatgataa gaacaaaggg
    22141 tatcttactc taaaggatat agatagttta agcaccaata gtgagtgtaa aagtgagctc
    22201 tataagttta ttagggttta tggcggaact ggtattggtg tggttacgaa aaaagggctt
    22261 agtgtggatg ttggtaagcc tgctataaat ccgactccac ttaaaatgat aaatcatgaa
    22321 ataagaaagc ttataggaga taattttgaa tctatacttg gaaatgataa agttttaaaa
    22381 ataacaatct ttgctccaca aggagaaact gtagcaaaaa aaacatttaa tccaagatta
    22441 ggtatagttg gaggaatatc tataataggt acaactggga ttgttgaacc tatgagtgat
    22501 gagggttgga agaagtctct atctatagaa cttcagatga aaaaagaaca aggcttagat
    22561 aaaataatat tggttccagg aaatcatgga gaacagttta taagagaaaa attaaactta
    22621 gatattaagt atgtagttag ggtaagtaat tttataggct atatgattaa agaagctcaa
    22681 agaataggat ataagaaaat cttaatggca ggtcatatag gtaaatttat aaaagtatct
    22741 gctggtattt ttaatactca tagtaaggta gcagatgcta gaagtgagat attagtggca
    22801 aacttagctt taatgggagc tagatacgaa tttttaaata aaataaatca atgcgtaact
    22861 actgaagaag ctgtagagct aataaataat agtgaatata gagaagtata taatatatta
    22921 agtaataaat gtagggagag agtaaagcaa tacttgaatg aagattcaga tgatattgat
    22981 gtggaagtaa tcatattttc aatggataag tctttattag gtaaatcgga taatacagat
    23041 gatttagtgg aggtctttat atgataaaca taataggtct aggacctggc aacttagact
    23101 atataacaaa aaaaggtgaa aatttaattt ccacttctga tgtattaatt ggtggtaaaa
    23161 gaaatttaga gtctataaaa aattttgaag gagaaaaaat agttctggat tctaatttaa
    23221 gagaaattat agagtacata aataacaata aagaaaagca aatatcaata atagcatctg
    23281 gggacccatt aatatatggg ataggtagat atctatctaa aaatattgat aataagatgt
    23341 taaacatggt ttctgggatt agttcattac agtacatatt ttcaaaaata tatgtagata
    23401 tgaatgatgt atatattaca agtagtcatg gcaaagtacc agattttgat tacatattat
    23461 cacataaaaa agtatgtatg gttacagatt caaagatagg accaaaacaa ataagtagag
    23521 aaataataga tagaaatcta aataaaatta ttgttgtagg agaaaatctg tcatatgaca
    23581 atgaaaaaat aacaatagca aaaccagaag agataataag aatagacaat tttgatatga
    23641 atgttgtggt gattttagat gaggaatagt gagtttataa caggtaaagt tccaattact
    23701 aaggaagagg ttagagcaat atcaataagt aagttaaatt tagtaaatgc taaaaacctc
    23761 atagacatag gggctggtac aggaagtgta agtgttgaag ctgcatataa ttatcctaat
    23821 ttaaaggtta tatctataga gaaaaatgat gatgctatag agcttataga gaaaaataaa
    23881 aaaaaattca acttaaaaaa tatagaagtg ataaaaggat atgctccaat taatctaggg
    23941 ttaaatgtta aagttgagtc tatttttctt gggggaacag gaaataatct ggaagaaata
    24001 atagaatggt ctaaaaaaaa tctcgttaca ggaggaagac ttgtagctaa ttttataata
    24061 atagatacat ttaatcaaac tttaaaactg cttaaaaagc atggatttaa agaaatagat
    24121 gtatgtgttt tgaatgtttc taaactagag aaactaggaa aaggtgaata ttttaaacca
    24181 ctaaatccta tatatataat atcttgtgaa aaaggggaaa atgatgatga ataaagtaca
    24241 ttttgtagga gcaggacctg gagataaaga gctgataaca ttaaaaggat acaaattatt
    24301 aagtaatgca gatgtagtga tatatgcagg ttcacttgtt aatcctgaac ttttggaata
    24361 ctgtaaagaa gattgccaaa tacataatag tgcacatatg gatttacaag agataataga
    24421 tgttatgaga gaaggtatag aaaataataa atctgttgta agactgcaaa caggtgattt
    24481 ttctatatat ggttcaatta gagaacaagt ggaagattta aataaattga atatagatta
    24541 tgactgtact ccaggagtaa gttctttttt aggagcagca tcatctttag gggttgaata
    24601 tacagttcca gaaatttctc aaagtgtaat tataacaaga atggaaggca gaactccggt
    24661 tccagagaaa gaatcaatac aatcatatgc taagcatcag acatctatgg taatattttt
    24721 atctgttcaa gaaattgaga aagttgtgtc taaattactg gaaggtggat atccaaaaga
    24781 tactcctata gcagttatat acaaagcaac ttgggcagat gaaaaaatag ttaaaggaac
    24841 tttaagtgat atagctgtaa aagttaaaga aaacaacata aataagactg ctttaataat
    24901 ggttgggaga tttttaggag aagagtataa taattcaaag ttatatgata aggatttcaa
    24961 acatgaatat agagggtaat attgctataa tctgtattac agaaaatgga aaaaaattag
    25021 ctctaaagat aaattctcta gtaaataact gtgttatata tcaagtaaaa aataaaaaaa
    25081 gtaatttagt tgttgaagaa acatgtgtac atacagttca aaagaagtta agtgattttg
    25141 taggagaaat atttagcaaa tttgattaca tagtatttat aatggctaca ggtatagttg
    25201 tacgaagtat agcaccatac ataataagta agtttagtga ccctgcaatt ttggtaatgg
    25261 atgaaaaagg caaaaacgta ataagtttat tgagtggtca tattggaggg gcaaacagct
    25321 tgacacagta cataagtagt cttattgatg ctaatccagt aataacaaca gctactgata
    25381 taaatcaaaa agcttcctta gatatgatag caaaaaaact aaatgcacat attgataatt
    25441 ttagagaaag tgtaaaagaa gtaaattcta tgctagtgaa taataaatgt gttggattat
    25501 atattgatga aggatatcaa gttgatacta ggggatttaa acttatagaa agtaaggatg
    25561 agttaagaga tagtttttca aacacatata tagaaaaatt agttgtagta agcaataaaa
    25621 taaattgtat tgaagatatt gagaatttaa acctagataa taaatttgaa cattgctata
    25681 gtaccaaaaa aataataaag gtaattccaa gagatatagt gataggaatt ggctgtagaa
    25741 gaaacacaga tagtgagctg atgtataatt cacttgaaga gtttcttaat aaacaaaata
    25801 tagatattag agcaataaac acagttggaa gtatagaact gaaaaaagac gaaaaagcaa
    25861 taattgaatt atcaaaaata ttgaaggtgc catttaaaac attttcagta gatgaaatat
    25921 ctaaggttga taatcttttt gataaatctg attttgttaa aaaaaatgtg ggcgtgtatt
    25981 cagtagcaga acctgtagca tatcttttga gcaaaggtaa tttgttagtt gaaaaacata
    26041 aatataaagg aataactttt tcagtgggga gaataaaaat atgatatacg ttgtaggaat
    26101 tggaccagga agtaaagata caatgacttt agaagcaata aaagcaatag aagattctga
    26161 ggctattgtt ggatataaga cttatataaa actaatagaa gaatttatac aagataaaga
    26221 aataatccaa aatggtatga ggcaagaagt tgatagatgt aaacaagcag tagaggaagc
    26281 aaaaaaagga aaaaaagtag cagttgtaag tagtggtgat gctggaatat atgggatggc
    26341 aggtcttatt ttagaattaa tatctaaaga agatgaagat ataaaagtaa aggtaattcc
    26401 aggtgttact gcaagtattg gtgcagcagc tatacttgga gctcctatta tgcatgattt
    26461 ctgtcatata agtttgagtg atttaatgac tccgtgggaa gtaattgaaa aaagattgag
    26521 attggctgct gaagccgatt ttgtaatttg tttgtataat ccaagaagta agggaagaag
    26581 tgaacatcta agcaaagcat ttaaaataat gggtgagttt aaaagtggag atacacctgt
    26641 tggagtggta aaagatgtag gtagagaaaa agaagaaaag cttgtatgta cttttgaaaa
    26701 tatggacttt gaaaaagtcg atatgactac aatggttata ataggaaata agtctaccta
    26761 tattaatggt gaattgatga taactccaag aggttataca gtatgatttt aattttagga
    26821 ggaacttcag atagtttatc tatatgtgat aagataaatg aactgaaaaa tcaaccatat
    26881 atcctttcag ttactacaga ttatggaagg gaacttgcac aacaacatgc agagaatgta
    26941 attttaggta agcttggaaa agaagatatg ctaaaattta taaaagataa taatatagta
    27001 aaaataatag atgccactca tccttatgca gttgaagtat ccaaaactgc tattacttgt
    27061 gcaaaactat tgaatataga ttatataagg tttgagagaa aatctttaat tgaagaaata
    27121 gattatgata ataaatttat agtagataca attgaagaag cctgtgaaat agcaaataaa
    27181 atagggacaa atatattcat aggaacagga agtaaaaatt tgaacagatt tatagaggaa
    27241 ataagtgata aaaatttagt tgttagagtt cttccaacga gtgaagtcat attaagttgt
    27301 gaaaaattag gtttaagtgc agataatata gtagctatga aaggtccatt tagccagtat
    27361 ataaatgaag aaacatataa acattacaat atagatttag tcataacaaa agaaagtgga
    27421 atagctggtg ggttcttaga gaaggtaaat gcatgtaaaa ctttcaatat accagtaata
    27481 attataaaaa gagaatcgat taactacgaa aatgtattta atgacataaa taaaattaaa
    27541 gacatacttg tgtaattttt aggaggagaa tatatgaaga aagccttatt agtagtgagt
    27601 tttggtacaa gctatcatga aacgagagaa aagacaatag atatatgtga agataaaata
    27661 aaaaattcgt taaaaagcca tgattttttt agagcttata catcaaatat gataataaat
    27721 aaaataaaaa aaagggatgg aatagaaata gataatccaa tacaggcttt agataaaata
    27781 tatgaacaag gatatgatga agtaataatc caaacacttc atattatatg tggcgaagaa
    27841 tttaacaaat taaaagaaca agtagatgga tattcttcta aattcaaaaa gattgtatta
    27901 ggaagacctt tgcttacaca tattgaagat tatcaggaag ctgtagaggc aattaagcat
    27961 caaattcctc atatggaaag taatgaagca gtagtattta tgggacatgg aacacttcat
    28021 gaatcacatt cagcgtatcc agcattggaa tacatgctta gggataatgg aataaatgct
    28081 tatgttggta cagttgaagg gtatccagaa atagaacatg taataagaag attaaaagaa
    28141 ggaaatataa aaactgtaaa tttgatgcca tttatgttag ttgcaggaga ccatgctata
    28201 aatgatatgg caggagatga agaagattct tggaaaacaa tattagaaga aaatggattt
    28261 aacgtaagag ttcatttaaa aggactagga gaaaattctt atatacaaga taaatttgtt
    28321 cgtcatgctg taaaatgtgc agagaatgcg aagttctatg gtataggagc aggaccagga
    28381 gatggttctt tacttacgat aaaagcggtt aatactttaa agaaactgga tattctatac
    28441 actccagaat caaaaaaggg tggagatagt ctagctttat caatagttag tgaatattta
    28501 cctgaatcag tggaaataaa gtctaggcat ttcccaatga gttttgatgg gaatgaaaaa
    28561 agtttagctt gggataatgt ggcacaagaa attatatcag atgttaatga tggtaaaaat
    28621 gtaggatttg ttacattagg agaccctatg atttatagta catatgtata tataatgaaa
    28681 agaatattag gagaggtaga agtcgaaact ataccaggaa tttcatcatt ttccaatata
    28741 gcatctaatc agaattttcc attagtcatg gataaagaag ctttagtggt aattccttgt
    28801 actatggaag atgataagat agagtatgct ttgcaaaatt ataattcaat agtattgatg
    28861 aaagtatata aaaattttaa agaaataata aaaaaattag aaaagtatga tttaatagaa
    28921 catgctattt tagtaagtaa ttcttcccaa gaaagcgaaa atgtgtttac agatttaaaa
    28981 gaagcgcacc ttgaagataa gatatcttat ttttctacta tactagtaaa taaagataat
    29041 aaaataaaat aggtgaaagc aatatttaaa ggagacttaa tatgaatata gtggttggaa
    29101 ctaggggtag taatctagcg cttatacaaa cagaatgggt aataaatgaa ttaaaaaaga
    29161 agtatccaga aattagtttt gagattaaaa tcataaagac aaaaggagat ttaattcaaa
    29221 atgtttcact ggataaaata ggtgataaag gattgtttgt caaagaaata gagcaacaat
    29281 tattagatgg aaaaatagac atagcagtac atagtatgaa agatatgcct tcttatttag
    29341 cagatggact aaaatttgct catacgccta agagagaaga cccaagagat gttctaatac
    29401 ttagagaagg atataaaaat ttagatgatt tacctcatgg agcagtaata ggaacaggaa
    29461 gtaaaagaag gaagtttcaa ttattgaaac aaagacctga tttaaatata gtacaagtta
    29521 gaggtaatgt ggaaactagg atacgaaaaa taaaagatga aaatatgcat ggtattgtgt
    29581 tggcagcatc aggaataatt agagctaatt tgcaagataa aataagttct tatttacctg
    29641 ttgatgttgt tattccagca cctgcccaag gagctttagc tatagaaatt agaagcaatg
    29701 atagtacgat agagggaata gtaaattcac tgaaggatga gaatacagaa atccaaatat
    29761 tggctgaaag aggtttttta gatggtgtaa atggaagttg tcatattcca atggcagctt
    29821 attgtgaaat aatccaagat aaaatacatc ttactggctt atatggagat tcagaaggaa
    29881 aaaaagtagt aatcaagtct atagatggag atatttcttc tcctagagag ttaggtttaa
    29941 agttagctaa acttgtattg aaggagtatg agaattatga agggtaaggt ttacttagta
    30001 ggggcaggac caggtgatta taaactcatg actttaaaag gtttagagtg tataagaaaa
    30061 tcggatgtaa tagtatatga tagattagca aatagtagtt atttaagaga agctaagcct
    30121 gattgtgaac ttatatatgt aggcaaggca tcaagtaatc atattttgcc acaagaagat
    30181 ataaatagaa ttatagcaga aaaagctaaa gaaggaaaaa ttgtaactcg tcttaaagga
    30241 ggagaccctt atgtatttgg cagaggggga gaagaagcag agactttagt agatgaaggt
    30301 atagaatttg aagtggttcc aggtataact tctgctatag gaggtttatg ttatgcgggt
    30361 attcctataa cacatagaga ccatgcttcg tcatttcatg taataacagg tcatttgaag
    30421 ggtgatgata gtggagaatt aaactggaat gctcttgcaa ataacaaagg tactcttgtg
    30481 tttttgatgg gaatttcaaa cctagagaaa ataagtgaaa acttaatgaa agaaggaaaa
    30541 gataaggata cacctgttgc tcttataagt tgggcaacta gatataatca aagagttgtt
    30601 acatctacac ttgaaaatgt ttatgaaact gctattaaag aagaagtaaa accgccaact
    30661 cttatagtag ttgggagtgt tgtaggactt agagaaaagt tgaacttctt tgaaagtaag
    30721 cctttatttg gaaaaagtat tgctgtaact aggtctagaa atcaaaatag tgtattggta
    30781 gaaaaaataa tggatttagg tggaaatcct atagaaatac ctactattaa ggtagaaaaa
    30841 atacaaaata atattaactt agaaaatgaa ataaaaaata taaataagta taattatcta
    30901 atactaacta gtaaaaatgc cgtagaaatc ttctttgaaa agatatatga aatgaatttt
    30961 gatttaagga tattatctaa tttaaaggta tgtgctatag gttctgctac atcaaatgag
    31021 ttgaaaaaaa gaggaattat agcggatata gtaccaaaaa aatttgtagc agagtcactg
    31081 tatgaagagt tagcgcctat tttaaatgat gacgataata tacttatacc tagagcagaa
    31141 aatgctagag attatctggt tgacaaactt aaggctattt gtgaagtaac agaagtacat
    31201 atatatagaa cagtaataga tgaggagaaa aaaggtgaga tattagatat tttaaattct
    31261 aatgacatag attatataac atttactagt tcctcaacag taaataatct tgttgaaatt
    31321 ataggaaaag aaaatattaa aatattagaa aaaataaaaa cactatctat aggtcctata
    31381 actacagaga caataaatag tttgggaata aaattgtaca aagaatctgc tacatctact
    31441 atagattcgt tagtagattt aattataaaa aattaatttt ggaggcttga atatgttaag
    31501 aagaccaaga agactaagag caagtaaagc aatcagaaat ttggtaagag aaactaaggt
    31561 gaatgtagaa gacttaatat atccactgtt tgtagtagag ggggaaaata taaaagaaga
    31621 aattaaatct ttaccagatg tctatcattt atcaatagat atgctagaag acgaaataaa
    31681 agaaataagt gagttgggaa ttgaacatat aatgttattt ggtgttccag atgaaaaaga
    31741 tgagtgtgct tcacaagctt ttaatgataa tggaatagtt caaaaggcaa ttagaaaggt
    31801 aaaagaaata aattcagata taaatgtaat aacagatgtc tgtatgtgcc aatatacaag
    31861 tcatggacat tgtgggattt taactgatac tggttatgtt gataatgata aatcattgga
    31921 gtatttggct aaaatatctg taagccatgc taaagcagga gctgatatgg ttgctccatc
    31981 tgatatgatg gatggaagaa tacaagcaat aagagaagct ttagatgaaa atggattaga
    32041 aaatgttgga atcatggctt atagtgttaa gtatgcttca aattattatg gaccttttag
    32101 agaggcagct cattcagcac catcttttgg agatagaaaa acatatcaaa tggacccagc
    32161 aaatagcaat gaagctataa tagagactga acttgatata ttggaaggtg cagatatact
    32221 tatggttaaa cctgcattat catatttaga tattataaga agagttaaag ataattttga
    32281 cttacctcta gcagcttata atgtaagtgg agaatactct atgcttaagt ttgctgtgaa
    32341 ggaaggttta ttaaatgaag atgtaattct tgaatcaatt acatctataa aaagagcagg
    32401 agcagatata ataataactt attttgctaa agacattgct aaactgttga aaaaataaat
    32461 tctagtagta taaagaatat caaaaagtat aggaattgta attaactatt atacagtagt
    32521 tttgtttaga catttattga agaataatag aaactaattt gcaatttcta tactttattt
    32581 atgtaactaa ataaaatttg gaggtagaag atgctatatc ctattaattt aaaattagat
    32641 gagttagatg tagttataat tggtggtgga gaagttgcat ataggaaatg taaaaatttt
    32701 ttagagttta acaaaaatgt tactatagtt tctaaacaaa ttttaaataa attttatgat
    32761 ttaaaaggaa atataaaaat aataaaagat gattataaag aaatatatat aaaagatgct
    32821 tctgtaataa ttgctgccac aaataataga gagttaaata tggaaatagg attatattgt
    32881 aaagcaaaaa ataagctagt gaatgtagtt gataatatag aaatatcgaa ctttactgtt
    32941 ccatcatata taaaaagagg agatttactt ataagtgtgt ctactggggg caagagtcct
    33001 tcattatcat caaagataaa aaaagaaatt gaagagagat atactgaaga ttatgaggag
    33061 tatttaaatg tattaggaga tataagaaaa aaggttataa aaaaatacga agataaaagt
    33121 aaaagaaaag atgtgcttaa tatgttggta acacttgacc tagaagaact taagaagttt
    33181 gatatataaa aaataaaata aaaaatgtta aaaaggtgtt gacggattat ttttaagatg
    33241 atatagtatt acttgtcctt aagaaaaggg caaacataa
//
LOCUS       DAGSWI010000032        32986 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.032, whole genome shotgun sequence.
ACCESSION   DAGSWI010000032 DAGSWI010000000
VERSION     DAGSWI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.032"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            29..1885
                     /locus_tag="MJF80_001373"
     CDS             29..1885
                     /locus_tag="MJF80_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBZ0280045.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1968..2208
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2274..2509
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2594..3811
                     /locus_tag="MJF80_001374"
     CDS             2594..3811
                     /locus_tag="MJF80_001374"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBZ0280046.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3928..4410
                     /gene="cdeM"
                     /locus_tag="MJF80_001375"
     CDS             3928..4410
                     /gene="cdeM"
                     /locus_tag="MJF80_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HBZ0280047.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            4675..5940
                     /gene="hisD"
                     /locus_tag="MJF80_001376"
     CDS             4675..5940
                     /gene="hisD"
                     /locus_tag="MJF80_001376"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBZ0280048.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6143..6895)
                     /locus_tag="MJF80_001377"
     CDS             complement(6143..6895)
                     /locus_tag="MJF80_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBZ0280049.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7013..7282)
                     /locus_tag="MJF80_001378"
     CDS             complement(7013..7282)
                     /locus_tag="MJF80_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280050.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7757..8851
                     /locus_tag="MJF80_001379"
     CDS             7757..8851
                     /locus_tag="MJF80_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBZ0280051.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8853..9803
                     /locus_tag="MJF80_001380"
     CDS             8853..9803
                     /locus_tag="MJF80_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBZ0280052.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     RGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVNHG"
     gene            9796..10980
                     /locus_tag="MJF80_001381"
     CDS             9796..10980
                     /locus_tag="MJF80_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280053.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DVYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            10993..12489
                     /locus_tag="MJF80_001382"
     CDS             10993..12489
                     /locus_tag="MJF80_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280054.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12512..13462
                     /locus_tag="MJF80_001383"
     CDS             12512..13462
                     /locus_tag="MJF80_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280055.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFS
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13483..14445
                     /locus_tag="MJF80_001384"
     CDS             13483..14445
                     /locus_tag="MJF80_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBZ0280056.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14817..15449
                     /locus_tag="MJF80_001385"
     CDS             14817..15449
                     /locus_tag="MJF80_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBZ0280057.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15860..15952
                     /gene="kdpF"
                     /locus_tag="MJF80_001386"
     CDS             15860..15952
                     /gene="kdpF"
                     /locus_tag="MJF80_001386"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBZ0280058.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            15993..17675
                     /gene="kdpA"
                     /locus_tag="MJF80_001387"
     CDS             15993..17675
                     /gene="kdpA"
                     /locus_tag="MJF80_001387"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBZ0280059.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17700..19757
                     /gene="kdpB"
                     /locus_tag="MJF80_001388"
     CDS             17700..19757
                     /gene="kdpB"
                     /locus_tag="MJF80_001388"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBZ0280060.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            19776..20402
                     /locus_tag="MJF80_001389"
     CDS             19776..20402
                     /locus_tag="MJF80_001389"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBZ0280061.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20639..21547
                     /gene="cysK"
                     /locus_tag="MJF80_001390"
     CDS             20639..21547
                     /gene="cysK"
                     /locus_tag="MJF80_001390"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBZ0280062.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21574..22164
                     /gene="cysE"
                     /locus_tag="MJF80_001391"
     CDS             21574..22164
                     /gene="cysE"
                     /locus_tag="MJF80_001391"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBZ0280063.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22324..22512
                     /locus_tag="MJF80_001392"
     CDS             22324..22512
                     /locus_tag="MJF80_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBZ0280064.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22566..23435)
                     /locus_tag="MJF80_001393"
     CDS             complement(22566..23435)
                     /locus_tag="MJF80_001393"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBZ0280065.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23632..24876)
                     /locus_tag="MJF80_001394"
     CDS             complement(23632..24876)
                     /locus_tag="MJF80_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280066.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINSKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            25167..25478
                     /locus_tag="MJF80_001395"
     CDS             25167..25478
                     /locus_tag="MJF80_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBZ0280067.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      25571..25663
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            25763..26557
                     /gene="thiD"
                     /locus_tag="MJF80_001396"
     CDS             25763..26557
                     /gene="thiD"
                     /locus_tag="MJF80_001396"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBZ0280068.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            26581..27369
                     /gene="thiM"
                     /locus_tag="MJF80_001397"
     CDS             26581..27369
                     /gene="thiM"
                     /locus_tag="MJF80_001397"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBZ0280069.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            27392..28024
                     /gene="thiE"
                     /locus_tag="MJF80_001398"
     CDS             27392..28024
                     /gene="thiE"
                     /locus_tag="MJF80_001398"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBZ0280070.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSVSFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            28163..28465
                     /locus_tag="MJF80_001399"
     CDS             28163..28465
                     /locus_tag="MJF80_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0280071.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(28531..29280)
                     /locus_tag="MJF80_001400"
     CDS             complement(28531..29280)
                     /locus_tag="MJF80_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280072.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(29274..30236)
                     /locus_tag="MJF80_001401"
     CDS             complement(29274..30236)
                     /locus_tag="MJF80_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280073.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNALSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDAIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(30240..30698)
                     /locus_tag="MJF80_001402"
     CDS             complement(30240..30698)
                     /locus_tag="MJF80_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBZ0280074.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNFPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            31053..31409
                     /locus_tag="MJF80_001403"
     CDS             31053..31409
                     /locus_tag="MJF80_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0280075.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            31481..32197
                     /locus_tag="MJF80_001404"
     CDS             31481..32197
                     /locus_tag="MJF80_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280076.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            32190..>32986
                     /locus_tag="MJF80_001405"
     CDS             32190..>32986
                     /locus_tag="MJF80_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280077.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYSLIGFQFNNLVAGLLSFLIIMFILNGISY"
BASE COUNT        12710 a         3597 c         5810 g        10869 t
ORIGIN      
        1 aataacattg aaaggtagtg gttagaatat ggatacccat aataaatatg taaattttat
       61 aaaaaatata cctgtacctt ttttatattg tagaattgta aagagacaag aagatataga
      121 atatcgagta gaatatataa gtaagggtat gggcaaagta ttacaattag aagaaggtat
      181 ttgtgataaa aatatactag atgtattgcc tgtatttaag tctaaaaaat atttcaaaga
      241 gttgttctca aatgaagtgg attgtataaa aaggtacatt ccgacactta aaaactggat
      301 aaatataaaa aaacagataa taggggattc atacataatt ttgtattttg gtaaaattgt
      361 atttgattat agacagataa tcgattcctt tgataaaaag gaaaaagtag cttatattaa
      421 agacgaagaa ggcatatata tagattgcag tgaaaattta ataccaatat taaataacaa
      481 cattaaaaca acgaaagata tctttggtaa aaatgatata gaagtatggg gagagaatac
      541 aggaaaatta tttagagatg attatagaga aggagtatct agtaaaaaaa gatttttaca
      601 aaatcttttt gaatatgaag aaacattttt tatggttgaa aaatattttt tgtatgatga
      661 agacgaactt ttaggtacta tagggatagt agacaatata atatatagtg gttacagtaa
      721 tagaaattat aactcaaaag atttaatgaa gatgatagaa cactcaattc cagagaatat
      781 gttttataaa gatgtgtatg gaaattatat tggatttaat tcaggttttt tgaatttagc
      841 ttgtatgaat aaagaagaat tattagggaa gaatagttat aaaatatcag aagaagaagc
      901 tttaatagat aagatatttg aaagtgataa gggtgtagtt gaaaataaaa aagtagttac
      961 atttgagtta aatataagta tgaatgatga aaacaagtgc attgagatta caaaaaggcc
     1021 gttttttgat agctatggaa gtgttattgg aataattgga acagcaaggg atataagtag
     1081 aagaaaaaga ttagaagagg aaatggataa aactaggatg gaattttttg caaatttatc
     1141 ccatgaactt agaacgccta taaacttaat atcatcttca ttacaagtga tagaaaaaaa
     1201 agaagcagat ttaatagaat caaatgatac tttaaaaaga aatttaggta taataaaaca
     1261 aaatggaaat agaatactga ggttagtaaa taatgttata gattttacaa aaatgcaatc
     1321 aggatattta gattttaaac cagaagaatc tgatataatt gcatttatag aggagatatg
     1381 tatgtctgta gctgattttg caagccaaaa taatatacaa cttacatttg atacagaaat
     1441 agaagaattt tcaatgttat ttgattcaga aaaattggaa agaataatat taaatttact
     1501 ttctaatgga attaagtaca ataagaaaga tggaaaaata aatatatttt tatatgtgaa
     1561 agataatgta tttaatatga aaatatctga tagtggaata ggaataccaa aagaaaaaat
     1621 agataaaata tttaataggt ttgaacagat tgacaatgaa ctttcttaca gagttaaagg
     1681 aagtggtatt ggactttcat tagtgaaatc tttggttgag ttgcatgagg gaagtatatc
     1741 tttaaaaagt caacttggta ttggaagtga atttatagtt tcattgcctg ttagaagtaa
     1801 aaataatatt gaaaaatata atcataaaag agaaatcagc aatgaactaa gtaaaaaatt
     1861 agaaatagag ttttcggatt tataagttta aatataaaaa ataaaatttt aaaaaagtat
     1921 tgacaattga tttagaaggg tttaaaataa attacaacat aataaataaa aaggcgatga
     1981 ataggaagag taaatctaat tcgattacca gagagaagag tccgttggct ggaagacttt
     2041 ttaaattaga gggattgaaa actacctagg agctactttc tgaagtcatc tgcattagta
     2101 gattttagta agaatagtcg ttagctgcgt taaagtgtaa agtgagtttt catgacatat
     2161 gaaaactaaa ttaggtggaa ccgcgggaat aatactctcg tccttatgtt ataaggatga
     2221 gagttttttg ttatttaaaa atataaacag taatcataat aattatagaa aatataaaag
     2281 caatgaatag gaagagtaaa tttaattcga ctatcagaga ggaaagttca caggctgaaa
     2341 gactttctaa gtttgagaaa ttgaaaacta cctaggagct gtggtctgaa gttactttaa
     2401 aaagttttag taagactaac cgttaactgc gttaaagtgt aaagtggatt ttatcaataa
     2461 atgataaaat caaattgggt ggaaccgcga gtatagactc gtccctttat atagggatga
     2521 gtctattttt attgcaataa aagattgaaa ataaaaaaaa atcggaaaat tataaattta
     2581 ggaggaaaat aaaatggaga ataaagtgaa aattggagta ctaggatatg gagttgtagg
     2641 aagtggactt atagatataa tagataataa taaagaaaaa agaaatatag aaatagttgg
     2701 aattttagtt aataatttgg aaaaacataa ggacaaaaaa tattctaata taataactaa
     2761 taatatagat gatatattta ataaagatat agatatactt gttgaagtca tgggtggttt
     2821 agaaccatca ttatcttata taaaaaaagc attaaacaat aaaattcatg ttgttacagc
     2881 aaataaagat ttgttagctg aatgtggaga tgagcttgca aaattagcaa gtgaaaataa
     2941 agtaagtatt aaatttgaag cttcagttgc aggaggtata ccagtattaa aaccaataat
     3001 agaatcacta gaaggaaata atatagatag tataaatgca attttaaatg gaactactaa
     3061 ttttatatta tctaaaatgt atgatgagaa tctatcttat gatatggcat taagacaagc
     3121 acaagaatta ggttttgctg aagcaaatcc agagtcagat gtattagggt atgatgctgc
     3181 tagaaaacta tcaatattat caactctagc ttatgacaat agagtatatt ggaaagatgt
     3241 gtatttagaa ggaattacag atatagatga aaaagatatt gaatatgcaa aaaaattaaa
     3301 ttgtaaaata aaattgattg gtcaaagtaa gtatgaaaat gataaagtaa gtgcttttgt
     3361 tagacctgtt ttagtagaaa aagataatat ccttgctaga atagacaatg agttcaatgc
     3421 tgtcatagtg aatggagatt cagtaggaga agtttctttt gtaggtaaag gagctggaag
     3481 tttagcaaca ggaagtgcgg tttattcaga tgttatagat ataatagata atagagtttc
     3541 aagtatagat tcttttacta aagataaaat acaagttaat aaaatagtta gagaaaagtg
     3601 tggagcactt cttagattta aaaaatgtaa taaagatgaa attctaaata tagttgagaa
     3661 ttgtttagta aaattcgata ttttaaatga tgatgatgaa cttgctatta tggtttatgc
     3721 tgattctgaa tatgaaataa acaacagttt atgtctaata aaagataaag gatactgcga
     3781 aaaaatgaat aaaatgttga aaataagtta aaaaagaaaa aatataagtt tatatgtaac
     3841 atattttgac gattttaata ctatatagta tgagatgaaa caaaacatct cataaaaaaa
     3901 ttaaaattaa agggagagat atgttatatg gaaaataaaa aatgttattc agaagattgg
     3961 tatgaaagag gagaatctac agctaaatgg ttccaaaatg atagagaaga atatgaaaga
     4021 gaagcatatg atgaagatag agaaagaaga ggttcaaact gtggatgttc agattcagga
     4081 gaaaatagac ctagaaactg tgaaagattt agaagagaag ctgagataag agaaagagaa
     4141 gcaagagaag cattctgtga atcttcagag aaaaagaaag aggcattagc atatgaatgt
     4201 gaagctagaa aattatggga agaagcagaa aaatactggg atgaatattc aaaatacaac
     4261 tataaaggaa tcgaatattt agcagaagct gctagattat ttgatgaagg tatggaatgt
     4321 gaagctagaa gaaatggaaa taatggagga aacaataata attgttgcca taaatgccat
     4381 aaatgtaatt gtaactgctg tagaaaataa aaaaattaat tgacaaatca atagttaaaa
     4441 tatatcatat tatgtataat aaaaagaaaa ttcagaaaaa aataatgatt agaagaaata
     4501 taagtatatt tattagacaa atagtgctca gttagtgagg tgagtacaat atgattaaac
     4561 tcccatcctc tagcttttga tagagggtgg gagtttttat ttattgatat atcttaaatg
     4621 aggataatat tgtaaaataa atgagtatga gattgtaaaa ttaggaggta aaaaatgaaa
     4681 agtattaaaa catcaagtat gaaagaaaag gtagatgaaa agatattaag agagcaagta
     4741 agttctataa tagaagatat aagaaataat aaggatgttg cacttaaaaa atataatgag
     4801 aagtttgata gaaatacaag agatgaattc agaattacta aggaagaaat aaaggaagct
     4861 tataagcatg tagatgacga atttataaat aacttaaaga ttgctgccaa aaatataaga
     4921 gaatttgcta aagctcaaaa actaagtttt gaaaaccctt ttgaaaaaga aatttatcca
     4981 ggtgtaatct taggtcaaac taatattcca atagagtcat gtcttgcata tgttccagga
     5041 ggtcagtatc cattgttttc aacagctctt atgcttataa tacctgcaaa agtagcagga
     5101 gtaaaaagaa tagtggcatg ttcaccaact atgaaaaata ctgaaaagat aaatcctaag
     5161 acattggtag ctatggatat agcaggtgct gatgaaatat atgcaactgg aggagttcaa
     5221 gctatagcag catttacata tggaacagaa aagattaatc cagttgacat aattgtagga
     5281 ccaggaaata agtttgtaac tgaagctaaa agacaatgtt atggacaagt agggattgat
     5341 tttgtcgctg ggccaagtga agttcttata atagcggatg aaacttcaaa tccagtttat
     5401 attgcagctg atttattagc acaatgtgag catgatttaa atgcaagagg aatattattg
     5461 acaaatagct tggaagtagc acaaaaagtt gaaaaaaata tagaaagtat gctaaaagat
     5521 ttgccaacaa aggacatagc atatagttca tgggaaaata atggagagat aatattggtt
     5581 gatgatatgg aagaagccat taaaataagt aatttttatg caccagaaca tttagaagtt
     5641 gcagtaaatg agtataatga tatttgtgat agattaacta actatggttc tttatttatt
     5701 ggaaatcttt ctgctgaggt gtttggtgac tatgtttcag gtaccaatca tacattacca
     5761 acacttaaag catcaagata tactggtggt gtttgggttg gaacttttat caaaacttgt
     5821 acaaagcaga tatttaatga aaaagctata caatcattag cacctgtagc tgaaaagctg
     5881 gcaaaagaag agggattata tgcacatgca aaggctgctg aggttaggtt taaaaagtag
     5941 taaaaaattt tattttggca gtcgagactt agtattagac tgagataagg aaaacgctga
     6001 gaacctgaat aaatactata ttttgtgggt ctcggcgctt cttttatatc tgtatataat
     6061 agaataatac tatctgtgtg tttaaatact aacttaatat ttcttttcta aataatgttt
     6121 atgaaatagt taatattttt agttaatact tgaaaaaaac ttttgctaac tgtgcaaatt
     6181 catctgcatt tgtcaatata gcagggtgtc ctccctgact aaaaatgtaa atacttgctt
     6241 ttgaaataaa agttgccatt tgcttgtact cttcttcaag attatgacga cacatttcgt
     6301 cttccttact tcctgtaaat aaaattggca tttctaattt acacaattct ttatgaaaca
     6361 aagggcgttt ctcgtttgca cattgcaata gagcttttgt atcaagatta acaacattct
     6421 cccaatcttt tccatgacac cattcataga attttcgtgc ttgaatatct tgctttgatt
     6481 tttcccttcc agtgattaga ttatctatga agttatcatt aagagtacgt ccatcaaagc
     6541 tatctgctat aattgcttca actaaatcgg gacgttctaa tccagtatta acagctgccc
     6601 atgctcctcc acttgttcca attaagccaa ctttaccaca attcaaatgt tcaatcagtg
     6661 ctattgtctg taaggcttca tcatgccata tgtcaggaga aaattgagaa actctatccg
     6721 actgtccatt acctaaaaaa tcaattaaga tacatctaaa attttctgca tataatggca
     6781 caagtgcttt aaacatatta gaagaaccag tattaccatg caggaaaatt atcggtttac
     6841 cttgtccata ttctttataa aaacaagaat taccttgata ctgaaaatat gacatatatt
     6901 ttcctccttt ttatttgtta agttaattat aacaatatag gtatgaatga aaaacctcat
     6961 gttctttctt agaataaaaa tgtgccacgc ataaaacgtg acacatttct aattataaat
     7021 tttgctgttt aagtattctt tgtatgcttt tctctgtcaa aaagtatttc tctgataact
     7081 gcttgatact cattccattt tggaaattta tacagatttg agaattacga gaagctaaga
     7141 cttgtttggt gtttgtgttt tcaccccaat gctttttgtg ctcttgcttt ttgggaatat
     7201 aaatcacttt accatctata tattcttgca cttgctcaat cagttcttta ggcagtaagt
     7261 caatcgaatt ttgatagctc attttgcacc tcctaaataa ttattcttta ggattagcaa
     7321 aggctactgc cactcacaat taaaaataaa attttttatt atgtacaata gcctttgcta
     7381 tgcacaagtt ccaattccca taaactaaat actcctttct ggtttgaaaa attcatctga
     7441 acattgattt cgcttttaag atagagttca atgacaaatt tacattttta ttatatatat
     7501 tcagatagat aattgcaact gtctttgtat gaggatattt tattatagtc aatactataa
     7561 tatgtattta tatcatcaaa ctaatatgaa cagttgaaag gaatatgaac aataaaactt
     7621 agtcaggaag aataattatg aagcagacaa tagagaaatt tgattttaaa gttttattga
     7681 aaatttattg acaaatttaa cttgtaaata ttatactaaa cttaataaat aaagtttata
     7741 aagtaaggtg ttgtctatga aaaatatagt tggaaaaccg caaacgataa aaaaagtaaa
     7801 tgaagattta ataaaaaaca ttataaaaaa tgaaggtcct ataacaaaac cagaaatttc
     7861 aagcattact gaattgagtt tagctacagt taataagatt gtagagcaat tagctttgaa
     7921 aaatgaagtt aaagtaagtg gactaagtga atctactgga ggaagaagag ctcaattatt
     7981 tgaaataaat gcaaacttag aacacataat agctttatat tattatagaa attgttgtat
     8041 aggggtagtt gccaatttat taggtgaaat aatttatcaa gaagaattta atataagaat
     8101 ggatacgtat gaaaacgtta accaagatac tttttttgtt atagatactc taataaaaaa
     8161 ggaaaaagga aacaatataa gggctattgg aattggtgtc ccaggagttg taaaaaaagg
     8221 tgtagttaaa aatataccaa ctataaaaag ttgggaaggt ataaatttag aaaatttaat
     8281 aaagagtaaa tacaatacta aagtgtttat agaaaatgat acaaatatta ctactattgg
     8341 gatttatcaa aaacagtata aagacaaata taaaaatatg gctcttatgt atttagaaga
     8401 tggtattggt tcaggtatag ttataaatgg tgaattgtat ttaggttcta caaattttgc
     8461 tggagaatta agttatctaa atatagaatt tggtaagtat aactctaaca acttaaatct
     8521 tgaagattat attatgagtt taagaaaaag atatttagaa agtaaagata ataaatttaa
     8581 gaaagaaatt ttatctataa tatcaaactt tataaggact cttgtatgtg tcctaaatcc
     8641 acaagctatg attatacagt gtagtatatt aaccaagagt gacatggagt ttatagaaaa
     8701 agatgtaagc aataatgttg gagaagaaaa ttttcctaaa ttaataaaag tagactcatt
     8761 gagagaagga agtataaatg gagtaattag tatgtgttta aaagaaactg actatcaata
     8821 ttccttatct aataaaaaag gaggatatta atgtgaaata tttggtttca atagatggag
     8881 ggggaacaaa aactaaattt tgtgtaagtg acttagatgg aaacatactt aaagaacata
     8941 ccactggttc aactaattat aagtcagttg gaattaaaaa aacatatgaa aatattaata
     9001 atggatttaa aaaaatatta aaagatttat atatcgatta tgacgatatt gagtacactg
     9061 tatttggtat atctggttgt gattctccta atgattataa gattataatg gatgaaatat
     9121 taaaaattgg gattaacaaa gaaaaaatat atctggcaaa tgatgcagtt ttagcttttt
     9181 atgcacaagc tgattcccct ggtttagtaa ttgtggctgg gacaggttca attatactgg
     9241 gaattaaaga agatggagaa atatatagag ttggtggatg ggggtataat tttagtgatt
     9301 tgggttctgg atatgatata gggagaaaat tattaaagaa agtattgctt tattgtgatg
     9361 aatgtcatga gtatagtgat ttatttagtt gtgtcttgga cttctttgga gcaaatagtt
     9421 ttgaacaact tgcatatatg attactgata taaatgataa tgttgaaata gcaaacttag
     9481 cttcattagt gattgattgt gccgaacgag gtgataaatt ggctattgaa atattaagag
     9541 aatcatcaac tgaactatct aaattagctc aagtaatatt gagaaaaata tctaatccaa
     9601 tgaaaatgga taaaagcgaa ataaatatag ttttgtctgg tggtacatta aataaaactg
     9661 tatatgctca aatgttaatg aataatttaa aaacagaaaa tacagataaa aatttaaaat
     9721 ttatatctca agaaaatcag ccagtatatg gggggataag acttgctatg gcattagcta
     9781 ataggagggt caatcatgga tagaaaaaag gtaatagtac aaatttttac tggaggtttt
     9841 aaggctaaag aaataaagtt taatgatata aaatgtaaac tactagaatt attggactgt
     9901 attgatatag aaaaggttat catgggatgg agtgtagatg aagaattata tgtagaaact
     9961 aaaaaactat taaataaata taatgttgaa ctatatcttt ggttgccatg tttttctgaa
    10021 ataggattac ttgaaaaaag taatttacta attgattata tggggaagga agtcaagggc
    10081 tatgctttac aagaggatga aaattttgag ttttattgcc caaataataa ggaaaatata
    10141 aaaaatataa agaaaatata tagtacttat tttagtgata ttaaatttga tggaattttt
    10201 ttagataaaa taaggtatgg ttctttttcg aataaactaa atggtgtttt taattgtttt
    10261 tgtaaagatt gtttaaatat atatgaaaaa aataatattg ataaagagtc gttaatgaga
    10321 gaaatgaata atgtgtctaa gggagttata gataatgaag atactccatt tggtataaca
    10381 gaatacatta atggaaaata caaatttaaa aataaaatat gggaagattt ttttgaaaca
    10441 aaaaatcaaa atatatacaa ggcattatta gagataagta gttattttaa aagtaaggga
    10501 ttgaaaatag gtatagatac tttttctcct tttatttctt acttcacagg tcaagattta
    10561 gaaaaattac aagaagtagc agattttata aaaccaatga tgtatagaac tacaaaagct
    10621 ccagctggtt taccatttga atttgaaagg tttattgatg aatccataca taagaataaa
    10681 gagattggaa aaagaaattt cttagatatt ttaggaatta aggagtatga aggagatgta
    10741 taccctatag aatttgtact agaggaaatc gattttatga caagaaatct taataaagat
    10801 gtatatgtag gagttgaaat aaataaaaag gaaattatat ctcctgttac acctaagtac
    10861 ataagtgaaa atatttcaaa tataaataag acaacagtaa aaggatatgt tttatcttgg
    10921 gatttgttgt cagcaccaaa agaaaatatt gatgaaatta taaaatggtt tcggaattag
    10981 tgaggtgata aagtgagtaa gatattggaa atgcaacaaa tatataaaag ttttggaaag
    11041 gtagatgttt taaaagggat aaattttgag ttagaaaagg gagaagtaag agctcttttg
    11101 ggagctaatg gagcaggaaa atctacactt attaaaattt taggaggagt tcattctcaa
    11161 actaaaggta aagtaatttt gactggtaat gaaataatgt ttaaaaatcc acatcattct
    11221 aaagaaaatg gtattagtat aatttatcaa gaattaagct tgataccaac gcttacagta
    11281 cttgaaaata tttttcttgg tagagaaatt ttaaaaggaa gtgtgttttt aaacaagaaa
    11341 gacatgcttg aggaatatat gaaaatttgt gaggattttg attttaatat agacccacat
    11401 atagtagttt ctaatttaag tgtagcaaac caacaaatgg ttgagataat gaaagctgtt
    11461 tcatgtaatt ctgaaattat cataatggat gaacctacaa catcacttac taataatgaa
    11521 aaagatgggc tatttaaaat aataagtaga ctaaagtcta aaggtaaaac aatagtctat
    11581 atttctcaca tattagagga agtttttaaa gtatgtgata gtgcaagtat aatgaaaaat
    11641 ggaattatga ttggaactta tgatgtagag gatttaacta aattaaagat aactcaactt
    11701 atgacaggaa atgataatca caaacttatt gagaagaagg attatacatt tgcaaaatat
    11761 gaagagccac cagtactaga agtaaagaat ataagctggt ctaacaaaat aaaaaatgtt
    11821 tcatttaaag tttatagagg cgaagttgta ggtcttgcag gtcttgtagg ttcaagaaga
    11881 actgaaatta taaatttgat atttggtata gataataaag atagtggaga tatttttatc
    11941 aatggtaaaa aagtagacat aacttctcct aaggttgcta taaaaaacaa aataggattg
    12001 attcctgaag atagaaaaaa tctaggatta attttagaac aagctatata cacaaactca
    12061 tcatcagtac aaattgataa attcaagaaa ttcttgttta tagatagaaa taaagaaatt
    12121 gagttttcta aaaaaggtgt taaggaatta ggaataaaag ttagtgatgt aaagcaagaa
    12181 gtaaaagagc ttagtggtgg aaatcaacaa aaagtagtag tttctaagtg gttagagaaa
    12241 gatttagatt taataatata tgatgagccg acaaagggaa tagatattgc tgcaaaagaa
    12301 gatattttca atacaatcaa agaattttca agtaaaggaa taggagtaat atttatatct
    12361 tctgatttag aagaagtaat aagagtaagt gatagaattt tagtaactag agaaggttct
    12421 attgtagatg atttaaaaaa tgagaatata caggttcaag atataatgaa cagtatattt
    12481 aatgtataaa aaatgaaaat ggaggactga aatgaagaaa gcttttgaga agttaaattt
    12541 aagagattat ggagtattat taggtttttt agctttatgt atagctataa gtattgcaac
    12601 tccatcattt ttaggtaaac aaaatatact taatctttta cgacaatctt ctataattgg
    12661 aataatttca gcaggtatga cttttgttat aatatctgga aactttgata tatcagttgg
    12721 agcagtagca gctcttgcag gtgctataac aatgaaattt gcgactggtg gaactaattt
    12781 gtttgcatcc atgctcttag gtgtagcgat ttgtgcagta ataggattaa tcaatggtat
    12841 agtagttgca aagataaatg taccatcatt aatagccact atggctatgg tcactattgt
    12901 aagaggattg ctgttgatgc ttacaggagg atatccaatt actgaaaata taccaatact
    12961 tgattacatt ggaaatggat atttgtttag tataccaata ccagtagtaa ttttctttat
    13021 agtggtgtta gcttcgttta ttgtacttaa taaaactaag tttggtagat atgtttattc
    13081 tgtaggaggg aatcaagaag caagtaaact taatggtata aatgtagatt ctcacaaagt
    13141 gaaagtgttt attattaatg ctgtacttgc aggaatagct ggaatcgtac taacaggaag
    13201 acttgggact gcaactgcga ttgcaggtga aggatatgat atggatgcaa ttgcatctgt
    13261 agtaatagga ggtacatcag tggcaggagg ttctggttct gtacttaaaa ctgttatagg
    13321 agtattactt atgagtgtta taaacaacag ttttaatctt cttggaatag atgtatattt
    13381 ccagtacata tttaaaggat taataatttt agcagcagtt ggatttgatt cttatagcaa
    13441 gaaaaaactt gcttcaagat aaaaaataag ggggattttt ttatgaaaaa attaatgaaa
    13501 catttagcat tgttgttatc acttgttatg atatttggtt tagttggttg ttcaaatggt
    13561 ggtgacaaag aaaaaaatgg agaaaagaaa atagcagtat tactaccagg ttcaacaggt
    13621 tactttgtag caactaaaca aggtatagat gcaaaatcta aagaattagg tgtaagcgtt
    13681 gaatatgcag atgctcaatg ggatgctagt aaacaattat cacaagctga agattttatg
    13741 gctaaagggg tagacatgat tgcattatgt ggagttgatt ctgctgtaag tgagagaata
    13801 gttaaagctg ctaatgattc agacgttcct atagtagcat ttacaaattc tataggaagt
    13861 aacccaactg gagaatttaa aggtttagtt acatatatag ggcaaaatga ggaagaaaca
    13921 ggagcattaa ctggaaaaat tgctaaaaac ttattaggag aaactggtgg gaaagctgta
    13981 ttaatagaag gagtaccagg aactactcca caagtattaa gaaaaaaagg tcttgaaaaa
    14041 gaattaaaag atagtaatat agaaatagtt tataatcaaa cttcaaattg ggagaaagag
    14101 caagctttaa aggtaacaga agatttaatt caaaagaaaa ctgattttaa tgttataata
    14161 tgtcaagatg ataattctgc tacaggtgca ggtcaagctt taaaagatgc tggtcttaag
    14221 gataaagtta aagtcatagg tttgggtgga agtaaagatg gattaaaagc tataactgat
    14281 gggttaatag atggtacaac ttatatgtct gctgtagaag aaggtggatt agtcatagaa
    14341 aaggcatcta aattccttaa aggtgaaaaa atagaacctg ttacacaaat taaacaagtt
    14401 gaagttaaca aagataatat ttcagagttc aagggtgaat ggtaaattgt catagataat
    14461 aaataagatt tttagagtta ctttagaaac tatataaagt aactcttttt ttaatttaat
    14521 ttagtgataa attattgaat agtatttcta aacaaataaa aaattaatat aaacaaagac
    14581 ttaattatac taaattataa gtaaaactaa tataaactta tggaaaaaag gaaaacgtct
    14641 aaagcattgg aattactata gtgtaggtgt ggtattaaaa aaacataaaa aaacataaaa
    14701 aaattgttga aaaaaatata gaaattttgt ataattaaag atttgaaaag tgcggtatac
    14761 aaagccaatt cttttgtata taatatatat aaattcagaa aaaggaaggg atatgtatgg
    14821 ccaggcggtt atttttagaa aggattggac ttatattttt aggttcagca atattagcat
    14881 ttggagttta taatttttat tatttaaaca acataacaga aggtggagtg ttaggtattt
    14941 tactactgtt aaaaaattta ttcaatatac aaccagcaat agcaaatgtt gtaatagatg
    15001 gtttactact tttagtagga tataaatttt tcggtaaaaa attccttata tattcaatag
    15061 tagcttctat aactttttca gtgttatatg acttatttga agctatagga cctctagtac
    15121 cacaatcaca aaatatgctt ttatcaacta ttttggcagg tgtaactgtt ggaactggtg
    15181 taggtatagt agtaaaagca ggttgtgcat caggaggaga cgatgcttta gcattagtaa
    15241 tttctaagac tacatctttg aacatagggc aagtttattt agcaacagat gttattgtat
    15301 tgttgttatc attattttat ctatcagcgt ttgatatctt ttattctttg attgctgtta
    15361 caattagtgg caaggtaatt gattttattt attatcatgg taaaagtttg gatatggata
    15421 taagtaatga tatagttcca gaatgttaat ataaattaaa taactttaga ttttattagg
    15481 ggtggcagat ttgccacctt ttttgtgtct aaatctttat agaaagtata atcccatcta
    15541 ggtttttgca aatctcaaat atagaattat gttggaatga ctgggaatac gattgccaga
    15601 tgtgcatttt aaaaaccaaa ttttatattt atgaaatact aatcattaat ggatgtttct
    15661 taatcctatc tttataccta aaatgataag ataaatgaag tagcaataca aagctaaaat
    15721 ttgtaaaaga gattaaatta aatggaggag ctaaaatgtt ggatatagtt atgatagcat
    15781 tgttattcat aggatttgtt tccatgaagt tacttgctga ttggtgtggg aaacaaactg
    15841 aaaaaaaata atgtaatgta tggagggatt acctatgtgg tttctggcag ttgtaattat
    15901 aatgttaata atatatttgg tatatgcgct tattaatcct gaaaaatttt aaacagtata
    15961 aggctaataa gtaagttgaa ttggggggaa caatttttat ggtgcagata gtaatagtgt
    16021 tagcgatttt tatgatttta gtaataccta tgggcaagta tttatatcat atagctacta
    16081 atcaaaaaac atttggagat agactatttg ataaagtgga caattttata tataaggtat
    16141 gtagtataga taagaaaaaa gaaatgaact ggaaacagta tgcattagct ttattattta
    16201 caaatgcagt tatggtattt ataggatata tcatacttag aactcaaagt atgcatattt
    16261 ttaatccaag tggaattaaa agtatggaac aaggactatc atttaataca ataataagtt
    16321 ttatgacaaa tacaaatctt caacattatt caggtgaatc aggactttca tactttagtc
    16381 agatgacggt gataatatat atgatgttta catcagctgc aacaggatat gcggctgcta
    16441 tggcatttgt aagaggtcta gtcggtaaaa agaaaacatt gggaaatttt tatgtagatt
    16501 taattaggat aacaacaaga gtacttttac caggagcttt aattattggg cttatattag
    16561 ttacacaagg agtaccacaa acttttgctg ggacagaaac agtgactact atagaaggaa
    16621 agcttcaaga tatagctaga ggtcctgtgg cagctcttga atcaataaaa catttaggaa
    16681 caaatggtgg tggattcttt ggttcaaatt catcacaccc atttgaaaat ccaacaataa
    16741 tatcaaatat agtagaaata ttatctatga tgatattacc aggagcttgt gttgttgcat
    16801 ttggtcacat gataaagaat aaaaaacaag gttgggttgt gtttggcgca atgtcaatca
    16861 tatttttaat aggtctagta gtgtgcttta aagcagaaag tgcaggaaac cctattttat
    16921 cacaattagg gcttaatcaa agtatgggaa gtatggaagg taaggaagtt agatttggta
    16981 tagcacaatc atcattattc acaacagtga ctacttcctt tacgacaggt acagttaata
    17041 atatgcatga tacactgact cctttaggag gattagtacc acttttaaat atgatgctaa
    17101 atgtagtatt tggtggtaaa ggcgtaggtc tgatgaatat gttgatgtac gctataatag
    17161 cagtattctt atgtggatta atggtaggaa gaacaccaga gtttttaact aagaaaatcg
    17221 aaggaaaaga aatgaagctt attgctttac ttattatatt acatccactt ttaatactaa
    17281 tgttctctgg tctttcagta gcaataccag cggggcttga aggaatttca aatcctggat
    17341 tccatggatt atcacaggta ttgtatgagt ttgcatcttc agcagctaat aatggttcag
    17401 gatttgaagg tctaggagat aacactatgt tttggaatat aacaacaggt atagttatgt
    17461 tctttggaag atatgtgtct attatagtac tactagcaat atcaagtttg ttagcatcta
    17521 aaaaagctgt aaatgaaagt ataggaacac ttagaacgga taattttaca tttacaatag
    17581 ttttagtact tgttgtttta attgttggtg ctttaacatt tttcccagcc ttagcgcttg
    17641 gacctatttc ggagcatcta gtgttgtggc actaaaaaat tgggagggag taaagtttta
    17701 tggaaactaa aaaaacaaag tttattacaa aagatatttt caaaacttcc attatagaag
    17761 cttttaaaaa attaaatcct aaatatatga tgaaaaatcc agttatgttt gttgtagaaa
    17821 ttggatttat agtaacattg atacttacaa tagtacctag tttgtttggt gatgaaggca
    17881 ataacttaag agcttataat gggattgtaa caataatctt atttataaca gtcctttttg
    17941 caaattttgc tgaagctgta gctgaaggaa gaggtaaagc tcaagcagat acacttaaga
    18001 aaactagaaa agatacagta gcaagggtta tagatacgtt aggaaatgaa aaaatgataa
    18061 gtgcaagtga gctaaaactt ggtgatattg ttttagtaaa tgctggagaa gttatcccaa
    18121 atgatggaga agttatagag ggtatagctt ctgtggatga gtcagcaata actggagaat
    18181 ctgcacctgt catgagagaa tcaggtggag atttcgcatc tgttacaggt ggtactacag
    18241 ttgtaagtga ctggttaaag ataagaataa caacaaaacc aggagaatcg ttcttagata
    18301 aaatgatagc tcttgtagaa ggtgcttcaa gacaaaaaac gccaaatgag attgctttaa
    18361 atactttgtt agtagggtta acacttatat ttatggttgt tttaatatct ctttatccaa
    18421 tggcaaacta tgcaggtgta aagattccaa tttctactat gatagctctt ttggtatgtt
    18481 taataccaac tactataggt ggattgttgt cagctattgg tattgcaggt atggatagag
    18541 taactagatt taatgtaatt gcaatgtcag gaaaagcagt agaagcttgt ggagacgttg
    18601 atactatgat tttggataag acaggtacga ttacttatgg gaatagactt gcatcagaat
    18661 ttatagcagt tggaaatgca gattcaaaag atttaataaa ttattctgtt atgtgttcat
    18721 taaaagatga tacaccagaa ggaaaatcta tagttgattt aggtatgaaa ttaggttcaa
    18781 caggtaagac aaaagaggca gaggaagctg agtttgtaga attttctgca caaactagaa
    18841 tgagtggtat taacttagct gatggtagag ctataagaaa aggtgcttat gatagcataa
    18901 taaaaagagt aaaagaatct ggtggtacag tgccagaaga tttagaagaa aatgtaaatc
    18961 gagtagctaa actaggagga acacctttag tagtgtgtgt agataatgag atatatggaa
    19021 ttatctactt aaaagataca gtaaaatcag ggcttgtaga aagatttgct agacttcgag
    19081 agataggaat aaagacaata atgtgtacag gggataatcc cttgacagca gctacaattg
    19141 caagagaagc tggggtagat ggatttattg cagaatgtaa accagaagat aaaatagatg
    19201 caataaaaaa agaacaatta gagggtaaaa tagttgcaat gacaggtgat ggaacaaatg
    19261 atgctcctgc acttgctcag gcagatgtag gtcttgcaat gaatagtggt acaacatctg
    19321 caaaagaagc agcaaatatg gtggatttag attcagaccc aactaagata ttggaagttg
    19381 ttgaaatagg aaaacagctt ttaataactc gtggggcttt gacaacattt agtatagcaa
    19441 atgatgttgc aaaatatttt gcgattatac cggctatatt tactcttgca atacctcaaa
    19501 tggagatatt aaatgtaatg aaactttcta cgtcatttag tgcgatatta tcagctttaa
    19561 tatttaatgc tataattatc ccatgtttaa tacctcttgc aatgaaggga gtaaaatata
    19621 gacctatgaa atcacaagct atgttaatga gaaatatgct tatatttggt cttggtggag
    19681 ttatagtacc atttttagga ataaaattaa ttgatttatt gattacacct ttagtaatgg
    19741 ttcttaattt aggttaataa atggaggata taaaaatgaa aacattaaag ccagcattac
    19801 ttgtaagtat tgtattgtta gttgtttgtg gacttgtata tccacttgtt ttgacaggtg
    19861 taagccaagt tgcatttaaa gataaagcta atggaagtat gatagaagta aatggtgtaa
    19921 aagtaggttc agaacttatt ggtcaaagct ttacagatgc gagattcttt aagggaagag
    19981 tatcatcagt aaactataat acttatacaa aagaagattt agtaccagat aaagatggaa
    20041 atacatctta tggaggtgta tcatcagggt cttttaatta tggagctaca aatccagaac
    20101 ttcatgatag agtgcaaaaa gatatagaaa agtttttaaa ggataatcca actgtcaaaa
    20161 gagaggatat accaacagat ttacttacag catcaggttc aggacttgac cctaatataa
    20221 gtccagcctc tgcaaaaatt cagataccag ctatagcaaa agcgtctgga atatcagaaa
    20281 gtaaattaca aaaaattgta gatgataata cttccaaaaa actttttgga gttttaggtg
    20341 aagatagagt taatgtacta aaggttaatg tagaggtcgc aaaaatatta ggtcttatat
    20401 agtattttgt agaattttag taacaaaata ttacgcaaga tttaatttaa aggttaaatt
    20461 ttaaagagtg aaaatcaata atgattttca cttttttatt tgaatattac taatatttgg
    20521 aataaagtga ttgtaagtat aaaatccaaa aaaaggttgc attatcaaaa tgataatact
    20581 ataatatatt tatcgttatc aatttgataa tagatttgga catataggag gaaatagtat
    20641 gttatataat aacgcattag agttgatagg aaacacacct gtagtaaggc tgaataattt
    20701 aggctgtact aatgggtgtt ctaacatata tgtaaaatta gaaaaagtaa atccagcagg
    20761 aagtataaaa gatagagctg tttatgaaat gttagagggc ttagagcaaa gaggagaatt
    20821 aaaaaaggga gatgtactag ttgaagcaac tagtggaaat actggtattg cattatctat
    20881 gataggaaaa ttaaaaggat atgaagtagt aatcgtaatg cccgaaacaa tgagcgttga
    20941 gagaagagat ttaatgaagg cttatggagc aaatttggtg cttacagatg gtaaagctgg
    21001 aatggcaggt tccatagaaa aagcaaatga acttttaaaa gaaaatccaa actataaaag
    21061 cctaaaacaa tttgaaaata aagataatcc aaatgcacat tataaaacaa cagcagttga
    21121 aattatgaat gatgtcaaag atttagatat attcgtatgt ggagttggaa caggtggtac
    21181 tttaatagga actgcaagat atttaaaaga acaaaatcca aatataagag taattgcact
    21241 tgaaccaaaa aaatctccag ctatatcaga aaacaagact ggacctcata aaatacaagg
    21301 aataggtgct aactttgttc ctgaaaatta tgattctagt gtagttgatg aagtaatatt
    21361 agtagatgat gaagatgcat ttgatacagt taaattactt gcagcaaaag agggaatttt
    21421 agtagggata tcttctggag caaatatatt tggtgctata gagatggcaa aaaagtatcc
    21481 agataaaaag atagttacag tagctccaga tggagttgat aagtatatgt ctatgggaat
    21541 attttaataa ttttatttga gggagtgaat actttgttta agaaaataaa taaagatatt
    21601 gaatatataa tgaagaatga tccagcagct aggtctaaga tagaagtttt tttactttat
    21661 ccatcagttc atgcaatgat aatgcatagg atggctcatg ctctttataa gaagaagaag
    21721 ctttttacag caagattaat atcccagata tccagattta tgactggcat agaaatacat
    21781 cctggtgcta aaatggggga aggtatattg atagaccatg gaatgggtgt tgtaattgga
    21841 gagacagcgg aagtaggtaa tagggtgaca atatatcaag gagctacttt aggagctaca
    21901 ggaaaagata ctggaaagag acatccaact gttggtgatg atgtattaat tggtgctgga
    21961 acaaaaatac taggaccttt aaatataggt tcaaattcaa aaattggtgc aaattctgtt
    22021 gtcgttaagg atgtacctaa tggtgcaaca gttgttggta taccagcaaa aatagtaaaa
    22081 ataagaaacc tagaacctgt aaaaaagaat aaaaaagaag tgtcctatga gtatgatgaa
    22141 ttagataatg tatattatat ataggattta tataactata aaataaactt tataaaaatt
    22201 attttaaaag gtattttttt caaatttgag gacataataa aaaagaactg aatgttacag
    22261 ttttggttca tcaatctttg ataaacaaga ttttaaattt gtagcgtcta ggaggatttt
    22321 attatgaaag caaatgttaa tcaagatact tgtataggtt gtggattatg tccatcaata
    22381 tgtccagaag tatttgatat gaaggatgat gggaaatcac atgttatagt tgatgaagta
    22441 ccaagtgatg ctgaagaatc agcagcagaa gctagagaaa gctgtccagt tgatgcaata
    22501 gatgtgcatt aataaaataa atgcctataa aaattgaatt ttcaattttt ataggcattt
    22561 gtcttttatt cagttagtat ttcaacacct gtttctgtaa ctaaaaccat acattcccat
    22621 tgagcagata atttaccatc ttttgtataa atagtccaat tattttcttc atcaacaaaa
    22681 atttcgtgag ttccttcatt taccataggt tctattgtga atatcatccc aggaaccaat
    22741 agcatctcag tccctttttt ggatacataa cttacaaaag ggtcttcatg aaagcttaat
    22801 ccaactccat gaccacctat ttctctaaca acagagtatc catttttctt tgcataagag
    22861 ttgatagcat cacctatatc tcctaaaaat ccccatggtt tggcaacttc aagacctttt
    22921 tggacacatt cctttgtaac ttgaactaat ttttttgcat tttcattaac attacctatc
    22981 ataaacattc ttgaagcatc tgaatagtat ccattaaaaa tagtagaaac atctacattt
    23041 ataatatctc catcttttaa gataatgtcc ttacttggaa taccatgaca aacttcatta
    23101 tttactgatg tacatacact tttaggaaat ccattatagt ctagtggagc agggatagct
    23161 ccattttgta gtgtaaagtc atgaaccaac ttatttatat cttctgtaga cattccaatt
    23221 tcgattttat ctgaaatata atccaaaatt tttgtgttta ttacagcact tttttttatt
    23281 tcctcaattt gactttcatt ttttataata tcatgagttg gtacaatatg acctaactta
    23341 gcaaatgaat ctattttatt atccatatcc atatgacatt ttttatattt tttagtactt
    23401 ccacaccaac agctatcatt tctatttaac ttcataactt ttcatccttc ttttattatt
    23461 tatttaaatt atgggttaca taaactattt taaaacataa ccattataaa tattataaca
    23521 taaatactaa gaaatccttt atagatagtg atttattcaa agtataaatt attatatggt
    23581 tattgttgat gaaaagagga tagttaaatg tattattgga tattagcgca cttaatagtt
    23641 tagttcaagt ttttcaagaa aatcatatgt tagaggacaa ttttccttta aagtattttt
    23701 tgaatcttca tcaagataat aatgtacaaa tgattctgca aaaaattctt ctataaactt
    23761 gcttatatat gcatgtgcaa cttgcttagg gtcataaagc ttatatcttt cctgagcaaa
    23821 tacttccata aattcttcag aatttgattt tttatttaaa acatttttat ctatagcatg
    23881 tgcagtttca tgtaattcta aattttttga tgaatgtaca tttgcatagc taggattact
    23941 ataacctatt ttagccacta tagaattatt tctatatata ccaggtacat ctttccaagt
    24001 cttgcctgat cttacccaac atggaggtaa ttgatatttt aaatcactaa attctggttc
    24061 atcagttaga tttgaattta ttaattttat ttttatatcc ttttctttaa gtgtctgtaa
    24121 atatttacta tttatttttg caagtctgtt tttcatattt tcaacttcat ctttatcata
    24181 attcaagtca tcaggaagta taagcatttg ttcaattata tctatactac tgtcttttat
    24241 attttcagat tcttcatcaa atttatattg aagagtatta ttttcataga tagttatatt
    24301 tgtattattt tctatagaat aagagtgaat atcctctaaa tctaaaatat tcatttgttg
    24361 taaaatatgt tttgtttcgc cactccatac tactaaagaa agtgaattaa aattaattag
    24421 tggattttct tttgctttta tatcattgct tatatctaca tcaaatacat attcaattga
    24481 gtcttctatg ttaagactat ctgtattctt tttaatatat aaatacgctt gttttaagtc
    24541 tatataaatc tcatctaaag attcgtcata aaattctgat aaattattag taaagagttc
    24601 ttcacttaaa agttcatttt tatcagttat actttcaatt aaaatattaa tttcttttat
    24661 catatcgtaa atacttattt ttctatttgc tttttcacta gatacattgc atggcgcaag
    24721 agcaatgcaa taaaaatctt tttctttttt ataaaaggct ttaagtgatt ttgataaaag
    24781 tgtaaagtct acacctatct gtaagttgac cttaagtgag tcattttgta ataattcagt
    24841 attatttctt ttagctgtat ttaaattgtt ttccataatc attgccccct ttttattagg
    24901 gtatcataat ttttttatat gtgtcaattt gtttttttga aaattataat ttttttgaaa
    24961 attttatttt ttataatttg tgaatttttt attatagaaa taaatatgtt attattatgt
    25021 caaaatttag cattagattc atgtagagta tgttttatac atatattata attaattctt
    25081 tgaagattgc atattcaaaa tacacaccat tttttttaat tctataatat aatatttata
    25141 tatttaatgt taggatgtga tttaaggtgt attttacata tattataaga tgtaaagatg
    25201 attctctata cactggatat accagtaata tagttagaag aatgaatgaa cataaattag
    25261 gaattaattc aaaatacaca agagcaaagg gatttgaaaa actagaagtt tattttgtga
    25321 ccaacacaaa aagcaatgct atgaagttag aatattatat aaaaaaatta actagaaata
    25381 aaaaattgtc tataataaaa aacccaagta tacttataaa tttaattgat aataaagaag
    25441 attatattat tggtaaggaa atagaacaat taacataaat atttatagta acattataat
    25501 gggtgcatat ggtgtaaaaa cttgacaata aatactattg taatataatg aagttataaa
    25561 tatagtaaat taaaatagct aggggagcca agagctgaga ggtataccga cccttacacc
    25621 tgatctggat aataccagcg taggaaagct tagtaaaaat tcaagtatgt attatttgtg
    25681 atatatgcta tttctaagct atacctattg taggtatagc ttttttaatt aagttattta
    25741 aattaaactt taggaggcaa caatgagtaa ttataaaata ccaacactta caatagctgg
    25801 atctgattca tctggaggag ctgggattca ggctgatttg aaaacgttta gtgcgattgg
    25861 tacttatggt atgagtgtaa taacagctat aacagcacag aatactaaag gagtttttgc
    25921 tgtagaggat ttaaataaga aaataataaa aaaacaaata gaggcagtct ttgaagatat
    25981 acctccaaga gctgtaaaaa taggaatggt ttcaagtcct gagattatac ttgaaatagt
    26041 tgagaatcta aagaaataca atccaaaata tttggttgtg gacccagtaa tgatatctaa
    26101 aagtggatat tatctattaa agccagaggc aaaagaaaac ttaattaagt atttgatacc
    26161 acttgcatac ataattacac caaatatacc agaggcagaa gaaataacag gaataaaaat
    26221 acataatgta gatgatatga agagagtagg agaagaaata ctacaacttg gacctaaatt
    26281 tgtgcttatg aaaggaggtc atttagatgg agaagcagtg gatattttag ttggtaaaaa
    26341 tatatttaaa gtttataaaa gtgaaagaat tgataaaaaa aatacacatg gaacagggtg
    26401 cacattatct tctgcaataa catcttatct agcattagga tatgaaataa cagaagcagt
    26461 aaatctaagt aaaatctata taacagaagc aataaaaaga agttttgata taggtcatgg
    26521 agtaggacca gtacatcatt tttataaatt tgagtaataa aaaaatattg gaggtagaat
    26581 atgtataatt taattaaaga tgtaaaaaaa ttaaatccat tggtaattca ttatactaat
    26641 aatgtaacta taaatgattg tgctaatgta actttagctg taggagcaag tcctcttatg
    26701 agtttttcat atgaggaagt agaagaaatg gtaagtgtag caaactctgt agtaataaat
    26761 ataggaacta tgaactcaaa tatgttggat ttattcctat tagctggaaa agcagcaaat
    26821 aagtacaata aacctgttgt tttggaccca gttggagtat ttgcaagcaa agcgagagca
    26881 gaacttacaa gtagactttt aaatgaagtc aaatttagtg ttgttaaagg gaatgtatca
    26941 gaaataaagt ttataggtgg ctttaatgta aggggtaaag gtgtagattc atttgatgaa
    27001 gaagaagatt ctactgagat aataagaaag attgctgaaa agcttgaatg tgtagttgta
    27061 gcaactggaa aaatagatat aataactaat ggtaaaggta catataaaat aaacaatgga
    27121 actgataaat taaaaggaat tacaggaact ggatgtatga ctgctagctt gattgcaagt
    27181 tttatggctg ttactgagaa tatattagaa gcagcaacaa tgggtgttct tacaatgtct
    27241 ttaagtgggg aattagctaa tttaaataat ccaccaatag gtacatttaa agaaaatctt
    27301 atgaatgcta tttatcaaat ggatatagat actttaagta aaaattctaa tattgaattt
    27361 ttaaattaat aaagagtaag ggagacttaa aatgattgat aaagaatctt taaaaaaatg
    27421 tttaaagttg tatttagtaa ctgactctga aatgcttaaa ggtagagact tttataaatg
    27481 tcttgaagat gcaatatctt ctggaataac aactgtacaa cttagagaaa aaaatgcttc
    27541 tggaagagag tttttaagaa aagctatgaa acttagagaa ataactaaaa gatatggtgt
    27601 taagtttata ataaatgata gagtggatat agcacttatt tgtgatgcag atggagtaca
    27661 tgtaggacag agtgacattg atgttagaga ggttagaaag cttataggaa acaataaaat
    27721 tctaggagta tcagcaagaa ctttagaaga agctatttgt gcaaaaaatg atggcgcaga
    27781 ctatttaggt gtagggtcta tattcacaac atctactaaa ttagatgcaa aatcagtttc
    27841 gtttgaaact gttaaagaaa ttaaagaaaa agtagacatg ccatttgtac taattggagg
    27901 tataaattta gacaatatag ataaacttaa atgtttagaa agtgacgggt atgctataat
    27961 atcagctata ttaaaagctg aagatatctc taaagaagtg gaaaagtgga cattaaaaat
    28021 ataaaattgg ctctggtagt gaaaaaaatg gaaaaatttg atataatcta agagtaaaat
    28081 ttaagcaatt ataaatgata aacttttcta catatacttt ttaatcaaga tatatgtaga
    28141 tttggttaaa aaggagctat aaatgattag gatattgtta gtgtgtgttg gaggcatgtc
    28201 ttctacccta ttggtaaata aaatggagaa ggatgcaaaa aaaagaaata tagactgtaa
    28261 aatatgggca gttggagaag gagatataaa atcggagctt gataactttg atattttact
    28321 tcttggacca caacttagat ttatgcttga tgacgtaaag tctatagttg gagatagagc
    28381 tccagtatct ataatagata tggtaaacta tggcacttgt aatggtcatg ctgtgttaaa
    28441 ttcggtttta gaaatattaa aataaataat aaaagtggat tctatataat agagtccatt
    28501 tttattattt gttattaata ggcttaaaac ttagcttacc atttcttttt ttaatctcca
    28561 catagcaagt gaaaaacttg aacagcacaa tattaaaaat agtaggataa caggcagtat
    28621 atctccaata agaatagtct caccaaatat taagctacgt aaagcgttaa taacatgggt
    28681 aaatggattg agcatcactg ctacttttaa tcctccagat aaactttcta tagggaaaag
    28741 agctgaactt aaaaagaaaa taggaagtac aattgcagtc ataattgttt cataaactat
    28801 ttcatttggc aataataaac ttatagaata agctagactt gataaaaaga aggctgtcat
    28861 aaatatcagt actatcataa gtaggattcc accaatgcca gattctatcc ttactgagaa
    28921 gaatatactt actatacaca aaatagttac ttctatgaaa gaaactaaga tagcttctaa
    28981 taattgacca aggacgattg aatacctact gataggtgca attaagactc tatagaaact
    29041 tccactgttc ttcattataa agtttataat accaccacta ctacaagaag aaaacacaac
    29101 cagtacaatg atacctggta gtataaaagc agtataatta ttgatattta tattattcat
    29161 agtttggttt gcaatagaac tataaagcac aagccaaaga aaaggttgta atatagtaac
    29221 tacaaatgag attggatttt taaaacgcca cttcatacta cgccataaga tatttaacat
    29281 tcccattcct cctttaagct ttttttagaa cttgtaagtg ctaaaaatac atcttcgaga
    29341 cttggctcta caatttctat tgcatcaaat tctatatcat gttctaaaag ccatttattt
    29401 attaaagtaa aagcagttct actatcgttt acacttataa aaattgaatt ttctcgtacg
    29461 ctcataaact ttactaaacc agtactttta attgcatctt tatatttttt tatatcttca
    29521 gtattatgga aagttatacg tagtatattt tgtctaatgt aactacgtaa acttgatggt
    29581 gttccttgtg ctaaatcctt tccattcttc ataatacaaa tattatcact caattgttca
    29641 gcttcttcaa gataatgagt agttagaaat atagttgtgc catactcatc tctaattttt
    29701 aataacatat cccacataga ttttcttgaa tcgacatcca taccaacagt tggttcatct
    29761 aaaaatagga ttttaggaga tgataccata ttcatagcta tgtccaatct acgttttaca
    29821 ccccctgaat atgatgaagt aggatatttt aagtaactag ataagtcaaa cttatcaatt
    29881 aaagagtcta ttctttgttt tgcaatctgt ggttctactt tatacatttt actttgaaat
    29941 accatatttt ccataaggga taaatgttca tcaatagaga tttgctgtgc tacacaagca
    30001 atttgagtac gtatccaaga aggattatct actaaatctt ttccaaacat tgttacattt
    30061 ccagatgtag gtttgtaaaa ggtagtcaaa atatttataa gagaagattt accagcacca
    30121 ttaggaccaa gtaaagaaaa aatttctcca gcatttacat taaagcttag agcatttaaa
    30181 gctttcacac catttttata ttcttttact aaattgtcta tttcaatcgc taacatatat
    30241 tattcctcct ctttaagtgg aaatattatc tctgtaatat agttatctgg gtttcctttt
    30301 atcaacatac ctggtccttt aataaatact tctctccaag gagtttgtat ttttatatta
    30361 ttttcatgaa tatatttaaa tatcatttca tacgtttttg gaaaattact ataagagcct
    30421 gtgtgagtaa aaaataaagc ttttgtttta ggaaattctt ttgtagttat acccatacga
    30481 tttggagttt cagcagttgg aacacataat tccatttctg caacaccagt tttttggtca
    30541 acatcatagt agcaaaagaa tggtgcaccg ttagactttc cttttatagc tttaaataca
    30601 tttggaaaat actttgttgc ctcagtcata ggtccattat aacgcatggt agccactgtg
    30661 actgattcga tatttttgat ttcaattttg caattcattg tttacctcct aatgtgtgtt
    30721 catattctta ctagattttt aagcattaat acatataaga ttttgattag ttgaaaacat
    30781 ataatcttat ctcgtttgct tgttttcaag tctaacataa cataaaatat atatcctctc
    30841 atttgatgct ccattttgta aagttattat ttaagcttta ctttagattt tttagcaaat
    30901 taaaataaag caatttataa aataagtatt tacaaattaa gtgtattaat cgtataatac
    30961 acttaatagg atactagata aagtctgaaa taaaggcgta tttttttaaa gtaagtgtac
    31021 tatcgtatta atacactaga ataggaggaa tcatgaattt tgaattagat aatacaactc
    31081 ccatttattt gcaaatagta aagtatataa agagacaaat agtaacagga gagttgaaac
    31141 caggagagac aataccatca agaagagaaa tggcacttaa tctaaaagta aacttaaata
    31201 cagttcaaag agcatataag gagatgggtg atatgaatat aataaatact tttaaaaatt
    31261 atcaaagcag tgtaacagtt gatgaaaata tattaaaaaa tttaaaatta gagcttataa
    31321 atgaatcact atctgttttt atagaagata tgaaagcaat aaatgttagc aaagaagaag
    31381 ttttaaaaat aatagaagat aagtattagt tttttaacaa atagaaaact tagtttagaa
    31441 agtttattta taagcatatt ggatgtggag gatagtaaat atgatagaaa taaagaatgt
    31501 cagcaaaaca tataaaagga tgcaagggct taaaataaga aagatagaag ctttaaaaaa
    31561 tgtaagtttt aatatagaaa agggaaaaat aactgcatta cttggtataa atggtgttgg
    31621 taaatcaact atgttaaaag caatagcagg acttataaac attgatagtg gagaaattcg
    31681 tatagatgga gaaaaaataa atgaaaaggt atacaataaa ttggcttttg ttccagatgt
    31741 acaatctcac tttagcaata caacaataaa agaaacattt gaatttatgg aaatttttta
    31801 ttcaaagtgg aataaagaaa aatcaaaaga aatgatggac atatttaaat tggatgaaga
    31861 tgaaataatt gataacttgt caaaaggaaa cattgcaaga gtaaaattaa tacttggatt
    31921 ttgccaagac ccagagtaca tacttcttga tgaaccattt acaggaattg atttgtttaa
    31981 gagggaagag tttataggag taatagcgca atatatggag gaaaatcaag ccattatcat
    32041 aactactcat gagatagtag agatagaaag tttagttgat gaggttgtaa ttcttgatga
    32101 agggcagatt ataacttcat ttaatgcaga agaattaaga gaaagagaag gtaaatctat
    32161 attagataag atgagggagg tatataagaa tgaataaact tctatatcta ataaatgtag
    32221 atttgagaag aagcaataag ttttatatag cttatatatc atttttttca ctaattacac
    32281 ttagtttaaa tttattcgaa attaataaat ttaaaaattc taaatttatt atgaacttag
    32341 caatagaaga ttttggaggt atattttatg gactagggat acttagtaat cttggtttta
    32401 tccaaagtat attgttcttt gggatattgg gactttttat atactatatt tttatgtgga
    32461 aaagagagtt cttttcaaat aatcaaagta tttatacact tatgatgtta cctcaaaata
    32521 agtttaagat ttgtatatct aaatcaatag ctttgataac aatgatatat ggttttttga
    32581 taagtcagac agcaacatta tttatatgta aatatatatt taatttcatt tttagaaata
    32641 tgcctataat caatatgaac tttgccaaag atttatatta tttaaacttg aagagtatac
    32701 caattgattt tatctctttt atggctatct atgtattttt actacttata gctatttcta
    32761 ttgtaaattg ctgtatatta tttatctttt cctatataaa taaattttat gtaattttaa
    32821 tcactttgct gattatagca ggcttatatt tttcaacaat atatataaat ataatcactt
    32881 tatttagaaa atattatagt cttataggat ttcaatttaa caatttagta gcagggttat
    32941 taagtttttt aattataatg tttattctaa atggaatatc ttattt
//
LOCUS       DAGSWI010000033        32923 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.033, whole genome shotgun sequence.
ACCESSION   DAGSWI010000033 DAGSWI010000000
VERSION     DAGSWI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32923
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.033"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            85..402
                     /gene="flgB"
                     /locus_tag="MJF80_001406"
     CDS             85..402
                     /gene="flgB"
                     /locus_tag="MJF80_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBZ0280078.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYVTFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMTTLQYNALISFANGKIAMTKSII
                     SGR"
     gene            410..814
                     /gene="flgC"
                     /locus_tag="MJF80_001407"
     CDS             410..814
                     /gene="flgC"
                     /locus_tag="MJF80_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBZ0280079.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKKVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            824..1138
                     /gene="fliE"
                     /locus_tag="MJF80_001408"
     CDS             824..1138
                     /gene="fliE"
                     /locus_tag="MJF80_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBZ0280080.1"
                     /translation="MPITQINNLMQGAKLDNLIGETKPLTLNKEEKKNFEDVIIDTID
                     KVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNKV
                     QI"
     gene            1163..2713
                     /gene="fliF"
                     /locus_tag="MJF80_001409"
     CDS             1163..2713
                     /gene="fliF"
                     /locus_tag="MJF80_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBZ0280081.1"
                     /translation="MIASFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDVQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTSGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVNENNQPINSGISVDKNKASEQALNKQLEKSILEILEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSATNNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVVAIGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            2726..3796
                     /gene="fliG"
                     /locus_tag="MJF80_001410"
     CDS             2726..3796
                     /gene="fliG"
                     /locus_tag="MJF80_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBZ0280082.1"
                     /translation="MSTEITNVNAFDLSDINESDKKEKIEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNAKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSMSGDDFKDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQDPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAAEALREEMEMVGVIKVTQVEAAQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            3789..4520
                     /locus_tag="MJF80_001411"
     CDS             3789..4520
                     /locus_tag="MJF80_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBZ0280083.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYNEYTQKA
                     KDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            4524..5843
                     /gene="fliI"
                     /locus_tag="MJF80_001412"
     CDS             4524..5843
                     /gene="fliI"
                     /locus_tag="MJF80_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBZ0280084.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVYATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAILPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRGIFE"
     gene            5875..6321
                     /gene="fliJ"
                     /locus_tag="MJF80_001413"
     CDS             5875..6321
                     /gene="fliJ"
                     /locus_tag="MJF80_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBZ0280085.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSIGKNNDSVQNQKITINYLLALNNSIMDLSEKLDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            6349..7554
                     /locus_tag="MJF80_001414"
     CDS             6349..7554
                     /locus_tag="MJF80_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBZ0280086.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNDKFEDNLYKTTDHKKV
                     KDRRETSKQNVSSDRPENSDNECHLDEDKSNHLEDENTYEKDCSNNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            7572..8180
                     /locus_tag="MJF80_001415"
     CDS             7572..8180
                     /locus_tag="MJF80_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HBZ0280087.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8207..9190
                     /locus_tag="MJF80_001416"
     CDS             8207..9190
                     /locus_tag="MJF80_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HBZ0280088.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9203..9406
                     /locus_tag="MJF80_001417"
     CDS             9203..9406
                     /locus_tag="MJF80_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HBZ0280089.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9406..10224
                     /locus_tag="MJF80_001418"
     CDS             9406..10224
                     /locus_tag="MJF80_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HBZ0280090.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10214..10909
                     /locus_tag="MJF80_001419"
     CDS             10214..10909
                     /locus_tag="MJF80_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBZ0280091.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            10921..11364
                     /locus_tag="MJF80_001420"
     CDS             10921..11364
                     /locus_tag="MJF80_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBZ0280092.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11379..11753
                     /locus_tag="MJF80_001421"
     CDS             11379..11753
                     /locus_tag="MJF80_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HBZ0280093.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEVLKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            11754..12419
                     /gene="fliP"
                     /locus_tag="MJF80_001422"
     CDS             11754..12419
                     /gene="fliP"
                     /locus_tag="MJF80_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBZ0280094.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12432..12701
                     /gene="fliQ"
                     /locus_tag="MJF80_001423"
     CDS             12432..12701
                     /gene="fliQ"
                     /locus_tag="MJF80_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBZ0280095.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            12713..14527
                     /locus_tag="MJF80_001424"
     CDS             12713..14527
                     /locus_tag="MJF80_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HBZ0280096.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYSVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFNMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            14536..16611
                     /gene="flhA"
                     /locus_tag="MJF80_001425"
     CDS             14536..16611
                     /gene="flhA"
                     /locus_tag="MJF80_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBZ0280097.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            16586..17602
                     /gene="flhF"
                     /locus_tag="MJF80_001426"
     CDS             16586..17602
                     /gene="flhF"
                     /locus_tag="MJF80_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HBZ0280098.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFS
                     KKDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLN
                     QEIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDAM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            17599..18477
                     /locus_tag="MJF80_001427"
     CDS             17599..18477
                     /locus_tag="MJF80_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HBZ0280099.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNVISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            18491..19192
                     /locus_tag="MJF80_001428"
     CDS             18491..19192
                     /locus_tag="MJF80_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBZ0280100.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19208..19630
                     /locus_tag="MJF80_001429"
     CDS             19208..19630
                     /locus_tag="MJF80_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBZ0280101.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEIRSNNHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELSLIRSCYKI"
     gene            19615..19800
                     /locus_tag="MJF80_001430"
     CDS             19615..19800
                     /locus_tag="MJF80_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HBZ0280102.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            19841..20614
                     /gene="flgG"
                     /locus_tag="MJF80_001431"
     CDS             19841..20614
                     /gene="flgG"
                     /locus_tag="MJF80_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBZ0280103.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            20635..21396
                     /gene="flgG"
                     /locus_tag="MJF80_001432"
     CDS             20635..21396
                     /gene="flgG"
                     /locus_tag="MJF80_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HBZ0280104.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21419..22300
                     /locus_tag="MJF80_001433"
     CDS             21419..22300
                     /locus_tag="MJF80_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBZ0280105.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDIGDDSEEFTKKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22303..22677
                     /locus_tag="MJF80_001434"
     CDS             22303..22677
                     /locus_tag="MJF80_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBZ0280106.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            22709..24112
                     /locus_tag="MJF80_001435"
     CDS             22709..24112
                     /locus_tag="MJF80_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBZ0280107.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISSGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDEAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFERYNQLIRLINRGMYKEAIQGFKLITEQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24228..26165
                     /gene="htpG"
                     /locus_tag="MJF80_001436"
     CDS             24228..26165
                     /gene="htpG"
                     /locus_tag="MJF80_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBZ0280108.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDTRNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKHYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLFIEL"
     gene            26553..27683
                     /locus_tag="MJF80_001437"
     CDS             26553..27683
                     /locus_tag="MJF80_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBZ0280109.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNSGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            27910..28932
                     /gene="splB"
                     /locus_tag="MJF80_001438"
     CDS             27910..28932
                     /gene="splB"
                     /locus_tag="MJF80_001438"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBZ0280110.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29114..30025
                     /locus_tag="MJF80_001439"
     CDS             29114..30025
                     /locus_tag="MJF80_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBZ0280111.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30040..30456
                     /locus_tag="MJF80_001440"
     CDS             30040..30456
                     /locus_tag="MJF80_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBZ0280112.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(30556..30948)
                     /locus_tag="MJF80_001441"
     CDS             complement(30556..30948)
                     /locus_tag="MJF80_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0280113.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31089..31484
                     /locus_tag="MJF80_001442"
     CDS             31089..31484
                     /locus_tag="MJF80_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBZ0280114.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            31887..32093
                     /locus_tag="MJF80_001443"
     CDS             31887..32093
                     /locus_tag="MJF80_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280115.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(32250..>32923)
                     /locus_tag="MJF80_001444"
     CDS             complement(32250..>32923)
                     /locus_tag="MJF80_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280116.1"
                     /translation="AIFIFNNKNTNNKLKEVTKNNEEIVPKNLEGLSIEQLIEIRDKA
                     IDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNYSNLTEKEKLIRQINIVNYEN
                     SINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSYEGLDFLIDHLEELCKFVDFD
                     VKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLSEEVKKEIKNWENNLNNLNMN
                     KNNSN"
BASE COUNT        13427 a         3511 c         5991 g         9994 t
ORIGIN      
        1 aagaagtcat tttttgtaat atagcaactt cattttttta ttaataagca aaataaataa
       61 aatatatttt gaggaggagt gagtgtgaat acatatgaca ttataaaaat gggattagat
      121 gcaactaatt taagaagtca gacaatagca aataatatag caaatataaa tactgaagga
      181 tataagagac attatgtaac ttttgaagaa acattaaatg atgctatgcc caaaatagaa
      241 gaaaaaacag ataattctaa aggattaaga gtggatggca ataatgtaga tatagaagaa
      301 gaaaaagtaa accaagcaat gacaacatta caatataatg ctttgataag ttttgcaaat
      361 ggaaaaatag caatgactaa atcaataata agtgggaggt aattaaaagg tgagtgtgtt
      421 cagtggaatg agaatatctg ctagtggatt aacagcagaa agcttgaaaa tggatatcat
      481 atcgtccaat gtagctaata taaatgccac aagaggtcca aatggagaag catataggag
      541 aaaagttgtt tcatttgaag aaaactatga taataaatta ggtatgctag gagtaaaagt
      601 tacatctgtt gaagatgata aatcacctct taaaaaagta tatgacccat ctcatccaga
      661 tgcaaagcca aatggatatg tagaatatcc aaatgtaaat atattggtag aaatgacaga
      721 tttaataaca gcttcacgag catatgattc taacgtagat acattgaatg ctcagaagtc
      781 tatgatatca aaagctttgg aaataggaaa gtagggataa attatgccaa ttacacaaat
      841 taacaattta atgcaaggtg caaagttaga taatttgatt ggagaaacaa aaccattaac
      901 tcttaataaa gaagaaaaaa agaattttga agatgtaata atagatacta tagataaagt
      961 taattttcaa caagtgacag cagatgatat gaaagaaaaa tttataaaag gcgaagatgt
     1021 ttctatgcat gaagttatgt taataggtca agaagcacag ctatctatgc aatatttaat
     1081 agaagttaga aataagattc atgatgcata tcaagaaatg aataaggtac aaatttagcc
     1141 ttttgaacag gagtaataat caatgatagc cagttttaaa aataagatta aagagcaagt
     1201 tgctaaatct aggacaaaga taagttctct aaaaaaggga actaaaatag cattaggtat
     1261 aggggttatt gctatatgtt tagcgctagt atttacagca atatctgcga gcaaaaataa
     1321 gtatgcagtc ttattttcag aactagatgt tcaagatgct gcagatatat cagcagaatt
     1381 agagtctaaa aagatagata ctaaggtaaa gggaagtacg atttatgtac ctagaacaga
     1441 agttgataag ttaaggctgg agttagcacc aagtcttaca agtggttcta aaggatttgc
     1501 actaatggat gatagttcat caataggact tactgatgaa gagtttcaga ttaagaagca
     1561 aagaatgata caaggtgaaa tagaaaaaac aataaagagt tttccacaag tggaagacac
     1621 tagagtacat ataacatttg gagagtcatc agtatttgag aaggaggctg ttccagggag
     1681 tgcagcagtt tatttgatgc taaaaccagg gacgacttta gaaaaaaacc aaataaagtc
     1741 tgttatggca cttatatcag gaagcgttac aaatatacca gagaaaaatg ttcaggttat
     1801 tgataacaaa atgaatttat tatctgaagg tattttggag gatgaaaatg ttaatgaaaa
     1861 taatcaacca ataaatagtg gcattagtgt tgataaaaat aaggcatctg aacaagcatt
     1921 gaataaacaa ctagaaaagt ctattttaga aatactagaa ccaatatttg gtaagggaaa
     1981 ggtgaaagct acagtaaatg ctgatttaag ttttgataca gctgaaaaaa ctgagataaa
     2041 aattgaccct gataaagttg caataaaaga gacaaggtcg aaaaattcat ctaaatctgc
     2101 tacaaataat gttcaaggtg tagatgctaa catgaataac caaggaaata acaatggtac
     2161 taatacagaa gatagtttag atgagagttt tgagtatgaa acaggtaaga ttgaaactca
     2221 tactatagtt gcgcctggag aacttaatag attgacagct tctgttgcaa tagatggaaa
     2281 aatagctaaa ggtgttcaag ctgatgtaga ggatattgtt aataatgcta taggtatgga
     2341 tggagcgagg ggagatactc ttagcgttgt ttcaatggta tttgacccag aaggtaagaa
     2401 agaggctaaa caagagctgg aagagattaa aaaagaagga tttaaaaaga acataataaa
     2461 agcagttatt ggagtagtgg cagtagtagc aattggagct attggatact tgatatataa
     2521 gaaaagaagt gaacgagaag atatggatta tgaagatgaa gattatattt ctaaatctga
     2581 ttctgttgat agaaaagata atcctctatt aggagatgac ccagaactga cattagagga
     2641 agctgttaaa atgtatgcag aagaaaagcc agaccaagta acagagataa taaaaacttg
     2701 gttaaatgag taagggggat agaaagtgtc cacggaaatt acaaatgtta atgcgtttga
     2761 cttaagtgat ataaatgaga gtgataagaa agagaagata gaaggtgagg gtttaaaaaa
     2821 agctgctgta ttattaatga cattaggacc aaatgtttct agtgatataa taaaaagctt
     2881 gcctgataaa caagttcaaa aaataggttt ggaaattgca aatatttctt caatagcttc
     2941 aaaagaaaga agagaaatct taaaagagtt tgtagaaata aatagggcaa aagattatgt
     3001 tatagaaggt gggcttgaat atgcaagaga gttgttaagc aatgctttag gaaatgccaa
     3061 agcaaacaaa ttacttgaag gtatatcaat aaatacaagc acaaagcctt ttaaacatat
     3121 tataaaacta gatgtaaaga aaatattaga aatacttagg aatgaaagtg cacaaactat
     3181 agccgtttta cttgcacata tggagccaga aaatggagca gaagttttat catacttaga
     3241 ttttgattta caaaatgaag tagctatgag gataagctca atgggttcaa tatctccaga
     3301 agtaataaaa gtaattgaaa gttcattaga agataaatta cactcaatga gtggtgatga
     3361 ttttaaggat tatggaggag tgtctacctt agttcaaata ttgattaatt ctgatagaaa
     3421 aacagaaaag aatattttgg gaactattga agaacaagac ccggatttag cagctcaaat
     3481 taaatctcat atatttgtct ttgaagatat tgtcaagtta gatgatataa gtatacaaaa
     3541 agtgttaaaa gaagtgacta tgaaagattt agtatatgcc ttaaaaggtg tggatgatga
     3601 gatttcagat gctatatata gaaatcaatc ttctagggca gcagaggcac ttagagaaga
     3661 aatggaaatg gttggtgtca taaaagttac acaggttgag gctgctcaac aaaagatagc
     3721 aaatatagta agaaaacttg aaagagaagg tgccataact attgtaagga attttggtgg
     3781 tgactttgtt gaataataag attattaaaa gtaaaaaagc attatataaa ggagtgctag
     3841 atttaaccat aatagataaa gttagagtgg gagaatcaaa tgtaaataaa gaaaatgatt
     3901 gtagatttag ggatgaggta ataaaagaag ctatagaatc tgctgagata aaaaagaatg
     3961 agatattaga agaagcaaaa atgaatgcac aaaatataga aaagcaagca tatgaaaaag
     4021 ggtatagtca aggtcaaaaa aatggatatg aagatggata taaagagtca tataatgaat
     4081 atacccaaaa agctaaagat gaagcagatt ttataaaaaa tcaggcagac ttaatactat
     4141 tacaagctaa tgagaagatg actgagtaca taaaagaaaa aaataaagag attgtaagac
     4201 ttgctattaa tatggctagt tctgttctaa aaaagcattt cgaagatgaa gaatctatga
     4261 gtgatttgtt aaagcaaatt attcaagatt atgaaggaag tagttcattt atagtaagat
     4321 gtaattcttt ctataaagat aatatagaga aagagtttga attattcaaa gaagaaacat
     4381 cactaaaatc aagtgtattt gttattgcag atgatagtat agataaaggc aatgctatca
     4441 tccaaaagga aaatggaaga ataatagttg gtatcgattg tgctatggaa aaactaaaag
     4501 aagaattgct tggtgaataa agtatgtata aaactgatat atctatagat tttgacaaaa
     4561 taaataagaa aatagaaaat ttagaactca ctatatcaga aggtagagtc aaaaagataa
     4621 taggacttac tgtagaagtt gaaggaataa aagcttttgt tggagagtta tgtgttatat
     4681 acaatcaagt caataaacca gtgaattgtg aagtagtggg ttttaaagat aaagaagtca
     4741 tattaatggc gttgggagag ttaacattga tagctcctgg ttgtaaagtt atttctaagg
     4801 ggataccttt aagtgttatg tgtagcgata atttactagg caaagtactt gatggtcttg
     4861 gaaatcctat agataactca gatgctgttt taggagatag atacaacttg aacaatgaac
     4921 ctcctgatcc aatgaagaga aaaaagatac gaaatataat ggaaacaggt gtaagagcaa
     4981 tagatgcatt tactacttgt ggtgaaggac agagaatagg gatatttgca ggaagtggag
     5041 taggtaaaag tacgactcta ggtatgattg ctaggaatgc aaaagcagat gtaaatgtaa
     5101 ttgcccttat tggagaaaga ggaagagaag tattagattt tattgataaa gatttaggtg
     5161 aagagggaat gaaaaaatct gttgttgtat atgcaacatc agataaagct cctttggtta
     5221 ggctaaaagg tgctcttact gctactgcaa tagcagagta ttttagagac cagggcaaaa
     5281 aagtaatact tatgatggac tcggtaacta gatttgctat ggcacaaaga gaagttggtg
     5341 tagcaatagg agagccacca gctcaaaaag gatatacacc atctgtgttt gctattttac
     5401 caaaacttat ggagagaaca gggacatcag ataaaggttc tataactgca ttttatacag
     5461 tattagttga tggagatgac tttaatgagc caatagcaga tacaacaaga gggatacttg
     5521 atggtcatat agttttatct agagacttgg caaataagaa tcattatcct tctatagatg
     5581 tattgaacag tttaagcaga cttatgaatg agattgcttc aaaagaagac ataaaaatag
     5641 catcttttgc gagagatatg cttgctgagt atagagaagc agaagatttg ataaatatag
     5701 gtgcatatgc ttcaggtaca aacaaaaaaa tagatgaagc aatctactat catgagcata
     5761 ttattaattt tttaaaacaa ggaataaatg aaaagagctc ttttaatgaa actataagta
     5821 gtttaagagg aatctttgaa taaacaattt aaaaaaatat ttgaataggt tgtgatggaa
     5881 ttggataaaa attttaagtt tagattacag aaagttttag acttaaaaat aaaagatgaa
     5941 gaagagataa aaatggaatt tgctaagatt caacaaaaaa aaatagatat agaaactaat
     6001 ttagaaaact tagaaagtaa ttacagcaaa tattctatag gtaaaaataa tgatagtgtt
     6061 caaaatcaaa aaataactat taactatttg ctagctttaa acaactctat aatggattta
     6121 tcagaaaaac tggataagtc gacaaatgaa cttgaaaaag ctagaaaaca gcttataagc
     6181 aaacagatag agagaaagtc attagaaaaa ttaaaagaaa aaaaatatgg acagtattat
     6241 aaagaagaac agttaaaaga acaaagcact aatgatgagt ttgcaagcat gagttatttg
     6301 agaaatagac aagttctata aaatagtagg aagtgaggat attattgtat gaagatacaa
     6361 gcagattctt ataatattat taaagatatt gggaatagaa aaaactcatc taagaataaa
     6421 aatataaaaa atgataaatt tgaagataat ctatataaga caacagacca taaaaaggtt
     6481 aaagatagaa gagaaacaag taagcaaaat gttagctcag atagaccgga aaatagtgat
     6541 aatgagtgtc atttagatga agataaaagt aatcacttag aagatgaaaa tacatatgaa
     6601 aaagattgta gtaataataa tgaagctttg cagcaattac tgaatctttt aaaagagaat
     6661 tataaaaaag ataaagatat aaacactata ttgaataata taaaaaaact aagtatatca
     6721 gatgaattaa aacaagaact taagcttaat aaaaatatat taaataagat tgcaaatcaa
     6781 aattcagagc taaataaagc taatataaat aactctaatg atatagaaaa tttagacata
     6841 gttaaattaa gagaactttt ggaggataat gctaataaag atattgaagt aattactaaa
     6901 actacagaaa atgatactaa aaatatagat ttgtatagtt ttgatagcaa tagaatggat
     6961 aatcttaact caaataaaca gaaagatgat agctctgata ttttggaaaa actagcaggg
     7021 gtaaatggaa ataagagtgg gaattttgga caagtaataa ataaaaatgt tgatatgaat
     7081 aaaattaaaa atgataatgt caatttaaaa cagatagaat caaatgtaat ggatgactct
     7141 ataaaagcta ttaagcatat gaaaacaaat gatatacaag aattgacaat taaattaaga
     7201 ccaaaagaat taggagatat gaatatacaa ttgttaaaag atggtgaaag tatgagagct
     7261 gttgtgactg ttttcaataa agatgtcttt gactctataa ataaaaacat agcagattta
     7321 aagcaacatc ttgaacttac taatgtaaat ataaaagatg tatcagtgca aatgcacagc
     7381 gataatacaa atacatctga tacctttgat aaagcatttg aacaacagaa tagacaaaat
     7441 aaccaagaaa acatgagtaa gaagaataca aatgataagc ataataatgt agtcatagaa
     7501 gatgacatta aagaaaatac tattgatgat gatagagtag atttactagc ttagtaaggg
     7561 ggaagtattg catgtacgga atagcaacgg ctttggcagg aactcaggga aactataggt
     7621 caaataactt aagccaactt gaaagaaata ttaatgatat tgccaataaa caagttagaa
     7681 gcaaaggaaa tcagacagta acagacaatg ggactccaat tataggacaa ggtgatgatg
     7741 ctgataaaga cttgtttatg aagatacttg tggcacagat gggtaatcaa gacccaatga
     7801 atccgcaaga cccaacagaa tatgtgtcac aattagcaca gtttgcaact ttagaaaaga
     7861 tgtcagctat gaatgagaag ctagatgtac ttttagcctt aagtaattcc actctaataa
     7921 actctgcttt aagtatggca acatcattaa ttggtaaaga agtagaagta tctggggaag
     7981 agaaagaaga tgaaaacatt gtaggagtag taaagtccac atatataaaa gatggggaag
     8041 tacacttaga attagaagta gatggaagtg acaaaactgt aggtgttaaa tatgaaaaat
     8101 tacttaaaat aggagaggta gatgaaaatt caggagaaga aaatgattct tcagaacacg
     8161 aatctccaga agaagcataa tattaaatga gaaaagaggt aaattcatga taaaagcaat
     8221 gtattcaggt gtaagtggaa tgaaagcaaa tcaaacaaaa ttagatgtaa taggtaacaa
     8281 tgtagccaac gctggaacta cttcttttaa gaaaagttca gctagaataa cagattcatt
     8341 ttatcaaact gtactatatg caagtgctcc aacagctgca ttaggaggaa caaacttagg
     8401 acaagtaggt gtaggtagta agatatcaag tataaataaa gatatggtac aaggaaatgt
     8461 acaaccaact aatagaccat ctgatttaat gatagatgga gatggatatc ttccagtagt
     8521 aagacaagga acagttatgt atacgagaga tggttctttt aatttagata caggatttaa
     8581 agcagctgat ggtgctttga agaatattaa aggtggaaga cttgtaaatg gtgatggata
     8641 tatgttacaa ggtatgattt atgatgggga atataaagct gatactggag aatttgttaa
     8701 aaagggtgat gctaaaggaa aagagcctat tgtaatacca cttcaaagta taagtcctaa
     8761 ggatggaaca gttcaaaatg tactagagta taatatatca aaggatggaa cagtagaatt
     8821 tttattgagt gatggtcaaa gaactcaatt tgtctatgat atacctgatg gtgctaaggt
     8881 aggagataca cctgctggaa tagataaatt aaaaggtaca atacaaaaag tgcaaacata
     8941 tgcattccaa aatccagcag gactagatgc agaaggagga aacttgttta aaccatcagc
     9001 aaactcagga gacccacaaa tagcaggagc tacaggcaca ataatacaag gggcaataga
     9061 agcttccaat gtagatatag cagaagaatt tacagaaatg ataatagcaa gtaggtcttt
     9121 ccaagcaaac agtaaaataa tatcgacatc agacgagata cttcaagaaa taataaatct
     9181 taagagatag taggtacata ttatgataag agtaacggat ttaaacggaa gaaaatacat
     9241 attaaattca gaccttatag tgaggattga ttctgtacca gaaagtagga ttctccttac
     9301 aacaggagaa aaaaaccttg taaaagagtc ggtagatgag atagtagata aagtaataaa
     9361 gtataaaaga aaaataaatg taggttatat taggagcgaa aaataatgaa aaaaacagat
     9421 tcactgacat ttattggact aatagtatct acagtattag ttttagttgg tgccgcaaaa
     9481 ggaagtagtt cagggttaaa gaattttttt gatgtttcat caatattaat cacagtgctt
     9541 ggttcatttg gagctctaat gattacattt actatagatg atataaaact tataaaaaat
     9601 gcattacaat actcatttaa gacaatgagt gtatcaaagt tagatttact agaacaattt
     9661 aaaacacttt ctaaaaaagc tagaaaagaa ggtcttttat caatagagag tgatgtaatg
     9721 ggaatagaag atccatttat gaagaaaggg ttagagctgt gtattgatgg attggaaatc
     9781 aatgagataa ggtccatctt agaactagag atagattctg ttgaagatac aaataacaga
     9841 tactctaaac tatttaaaac ttggggaacc tttgcacctg cttttgggat gttgggaaca
     9901 ctaataggtt taatacaaat gttagctgac cttacatctc cagatttaat agcatctggc
     9961 atgggaaagg cattaattac aacgttttat ggttctttac ttgccaatat cgctttgaac
    10021 ccaattgcat ataatataga tgagaagact gaaaaagaaa tttatgtaaa ggaaatgatg
    10081 ttggaaggta taataagcat acaatctggg gagagcagta tagtagttga ggaacgttta
    10141 gccacatacc tttcaaacaa tgagaagtta gaaattatga aatccaataa aaatacagaa
    10201 agagcaatga gcaatggcgc gtaaaaaaaa taagaaaaag gatgagataa atccagatgc
    10261 atggctggct acttatgcag atactataac actgattctt acatttttcg tattattgta
    10321 tgcaacatca gttgtagata tggaaaaatt taaaaacata gcagatgcgt taaaaggtca
    10381 gttttctggg acaagtatat ttagtacggg ttcaagtaca agtgagaaat ctccaataga
    10441 cagtgtttta gtggataaaa atgataaatc aaatttagaa caggatttaa aagataaagt
    10501 taataattct aacttacaaa atagtgtaac agtcaaagat gatagtagag ggattttact
    10561 agagttagat gattcaattt tatttgactc tgggatttct gagttaaaag tagatagtaa
    10621 agctactctt aataaagtat atgacatgat aaagatgatg aaaaataaga tagttataga
    10681 aggccatact gataatgtac ctgtaaaatc aagtacacat gaatccaatt gggatttatc
    10741 ttctagtaga gcagtaagtg ttgtaagata ctttgtagaa gaaaagggca tggaccctag
    10801 actattttcg gcaacaggat gtggagagta tcaacctttg gtttcaaatg aaacagcaaa
    10861 gggaagggct gagaatagaa gagttaatat acttataact attgactagg aggaagaggt
    10921 ttgttcaaaa ataaaaaaat tattataatt attttgatac tgattatttt ctcagcggga
    10981 gcaggttttt ttatattagg agaaggtgga aataaacaag ttgacaagat tgcaggtttg
    11041 tttattccaa caggaaaagg taaagatgaa gtaaagaccg aaaatcttga cttagaagag
    11101 atggtattaa agttagctga tgaaggtgta agatatttaa aagtatcaat aactgtatct
    11161 tatgattctt catataaaga tatagaaaaa aatacgcctt taattaggga taatattata
    11221 aattgtttta tggcaaaaag ggcagatgat tttactgctg ataatttaaa gaacataaaa
    11281 gaagaaatta aaaatacatt aaatatacaa ctaggtgaaa atgtaattca aaatatatat
    11341 ttcacaaata tagtagtgca ataaaaggat ggtaagacat gatactttat ttactaaaaa
    11401 tattaatatg tatcccaata attttaatat tgatagtctt atctttaaaa attagccaag
    11461 gaactttatt aaaaaaatat aatgataaat atgtaaaaat actagatatt gtaaatataa
    11521 gtaaaaacaa tagcataata attttaaaaa taggtgaaga aggatgcatt atggcatctt
    11581 cttcttctag catgagtaaa ataaaagatt tgaccaaaga agaagtatta aaaatagaaa
    11641 acgacattaa agaaagaaat tttaaaattc aagattttaa aatggaaaga attaaaaaga
    11701 aatatgattt cagtagatat attggaaaat ttacaaaaaa ggaagataag taaatggaac
    11761 tattatcaaa attacctttg gctgatgtac catatacaag ttcaatgcaa ttgtttttat
    11821 ttctaactgc attaatgttt ttaccatttg taatgttcat gatgacaagc tttgtcagaa
    11881 tagttataag tttatcattt ttgaaatcag cattaggagc acagcaagca attccaagtc
    11941 aaattttggt aggacttgca atagtattga caatatttat aatgagacca gtattaaatg
    12001 agataaacga aaaggcatta caaccatata tgaaagaaga agttactatg gaagaagcta
    12061 tgaaggaagc agaaggacct attaaagagt ttttattaac acaaaccaga cagacagatt
    12121 tagatttatt tgtagaacag gctggtttaa aagaaaagaa attaactcgt gaaaatatac
    12181 cactatctgt tgtagttcca gcatttgcta taagcgaact aaaaactgct tttcaaatag
    12241 gttttttaat atatatacca tttttgataa tagaccttgt tgtggcaagt gttttgatgt
    12301 ctatgggtat gtttatgttg cctccagtca tgatatcatt accatttaag ttattattat
    12361 ttgtaatggt ggatggatgg aacttgatag taaaaacatt gatattggga tttggatagg
    12421 agaaaaatag tatgagtgaa tttatgataa tgagtgttct taaaaatggc atgtttacag
    12481 gagctatgat tgcagctcca gtactcattg tttctcttgt agttgggctt ttaataagtg
    12541 tgtttcaagc tacaactcaa atccaggagc aaacattgac ttttgtacca aaactaatag
    12601 ctattctttt agtagtagct atcggtggaa gctggatgtt acatactctt attagtttta
    12661 cttctgagat attcaatatg atatctctta taactaaata agtaggatac atatgatttt
    12721 tgtatttatt agaatagtag cattctttgg aacactaaga atactatttc cagctggtac
    12781 acctgcttta tttaagtcac tctttgcaat cattatatca atattgattt caagtactat
    12841 gcaaattgaa tatgcaacaa atatagacaa cattgtattg tttaccttat atagtgtaaa
    12901 tgagaccata actgggatta tgcttggata tataaccaac ctatgttttt atagtatcag
    12961 aatggcgggt tctatgatgg accaacagat gggattatca atgattaata tgtttgaccc
    13021 aaattcaatg actcagacaa ctttaattga taatcttatg aactggacag cacttatgat
    13081 attttttaac atggatggtc atcatgtttt aattagaggt ataaggtata gttttgagtt
    13141 aattccaata ggaaaaccat ttgtagataa taacatagat tatataataa acatatttgt
    13201 gcagtgcttt ttaactggat tcaaaatagc aatacctatt gtactttgtc tgcttatggc
    13261 agattttatt ttagggctta tatcaagaag tgtacctcaa ttaaatgtaa tgatagttgg
    13321 tatgccatta aaaatattgg ttggtattgc attatttatc atatcaatac ctttaattgt
    13381 caatcaaata agtcatttat taagtcaaat accaaagatg tatgagggaa cttttgcact
    13441 agctcctatg ttttttatgg gttcagcaga taaaacagaa gaagctactc caaagaaaaa
    13501 gggagaacaa agaaagaaag gtaatatagc aaagagtaga gaattacccg ttgcaatgac
    13561 tctacttgca ttcaccttat tagtacctac attattttca tatgtagtag atacacttaa
    13621 atcttcgctt aattattttt tgagtctaga tttttatatg aacataaatt attcaaacct
    13681 agagaaatta gttatagcag gattaatgga tttttttaag atatttttac caatagctat
    13741 tcccttttta gttttaggca taatagcaaa cctatttcag gtgggaatac tgtttactgg
    13801 tgaaacttta aaaccaaatt tatcaaaatt aaatccaatt agtggattca aaaacatgtt
    13861 ttctatgaga agtttgtcaa ccttaataaa ggatacagcg attatatcta ttttggctta
    13921 cataggatat acattcttcc aagataatta tttagatata ttaaagttag gtaacatata
    13981 cctaccaact ttaatgtaca cagttaaaga cttagtatat tcaatattga gtaagatttg
    14041 tgtagctatg atagctatag cagttgcaga ttatgtttat caaagataca gtcataaaaa
    14101 gcaactgaga atgacaaaac aagaagttaa agatgaatat aaaaactcag aaggagaccc
    14161 agaagtaaaa gctaagataa aacaaaaaca aagacaaatt tcgtctcaaa ggacaatgca
    14221 agcagtacca agtgctactg taatagtaac caatccaacc cacttatcta tagcagtgag
    14281 atatgaaaaa gggaaagacc aagctccagt agtggtggca aaaggtgcag attatttagc
    14341 ttttaaaata agggaaattg caaagggaaa tgacatacct ataatagaaa ataaacctat
    14401 tgctaggttg ttatataagc aagtagaaat agaccaagaa attccagagg acatgtatca
    14461 agcttttgca gagatattag tagcagtata taaaatcaaa aatagataca aagtccccaa
    14521 aaggtaggat aagtaatgaa taagtttaat ttaaaggaaa atatggacgt aatagtggca
    14581 tttggtgttg tagggattgt tcttatgatg atagtgccgt tacctacatt tatattagat
    14641 attttactgg caattaacat aagtttatct gtgctaattt tactaatgac actatttaca
    14701 acaaatgtac ttgatttatc tatatttcca acagtgttac ttgtgacaac actatttaga
    14761 ttgggattaa atctttcatc aactagattg attttaacac agggatatgc aggtgaagtt
    14821 atagaggcat ttggaagttt tgttgtaggt ggaaattacg tagtaggaat aattatattc
    14881 ttaattatat taattgttca atttgttgtt ataaccaatg gttctggaag agtatctgaa
    14941 gtaacagcta gatttacgtt agatgccatg ccaggtaagc agatgagtat agatgcagat
    15001 ttaaatgcag gagttataga tgacaaaact gctagaaaaa gaagaaaaga gcttcagcaa
    15061 gaagcagatt tttatggctc tatggatgga gcaaataagt ttgtaaaggg agatgccata
    15121 gcaggtctta tagtaacagc aataaacata ataggtggaa ttgtaatagg agctgtaatg
    15181 ctgaatatga ctcttatggg tgcagcacag acatatgtaa gacttactat aggggatggt
    15241 cttgtaagcc agatacctgc tctattaata tctacatcag caggtataat cgtaacaaga
    15301 gcttctactg atgaaaactt tggtggatta gtaggtaaac agttaacagc aataccaaaa
    15361 gcagtaatga tgactggctg tgttctaatc gtacttggta tgatgcctgg attacctaaa
    15421 ccagcatttt tttcactagg tataggtgct atagcagcag gttacttttt aggaagagaa
    15481 aagaaagata ctttagaaga cttggagttt gaaaaacctg atatggtaaa tccagataaa
    15541 aatgaagtag aagatgtaac aaacttaata aatgtagaat caatagaagt agaaattgga
    15601 tatggtttaa tatctttagc tgatgaatca gcaggaggag atttacttca gagaatagca
    15661 tcaataagaa gacaatgtgc aatagacatg ggtatagtcg tacaacctat aagaataaga
    15721 gataatttac agttaaatcc aaaccaatac aatataaaaa taaaagggaa tgtagttggt
    15781 gggtatgaga taatgcctaa tatggtttta tgtatggatc ctatgaatca aggatttagt
    15841 atatttggaa taaagacaat tgagcctact tttggaatag aggcactttg gatagaaagt
    15901 gacaaggtag aagatgcaga gctaaagggt tatacagttg tagattcatc cacaatactt
    15961 ataactcact tactagatat aataaaatca aatgcacacg aattactggg aagacaagaa
    16021 gttaagacta ttatagatgc agcaagagaa aattatagtg ctgtagtaga tgaattgata
    16081 ccagatttaa tgacacttgg tgaaattcaa aaagtactta aaaatctgtt aagagaaaaa
    16141 gtaaatataa aggatagagt tacaatactt gagacacttg cagaccagtc tagaaataca
    16201 aaagatatag agcttcttac tgaatatgta agaatagctt tggctagaag tatttgtaat
    16261 aatttagttg atgaagataa ggcaattgta gtggctacat tgtctccaga aactgaagaa
    16321 ttggtatcta ataatttaca aaggtctgta aatggaactt atccagcagt tgacccagaa
    16381 aatacaaata aaatatttga gagtatacaa gaagtaatga acaatgtata tttcaatcat
    16441 aatataccag taatagtagt atcaccaaag ataagagctc catttagaaa attagttgaa
    16501 attgtatatc ctaatttgac tgtattatca ttaaatgaaa tacctaacga tattaaaatc
    16561 aaagcagaag gtgtggtgag catatatgat gacgaaaaag tatacagcta acacaattca
    16621 agatgctatg aatctagcca aaatggaatt gggtgacaat attacattaa tagataaaaa
    16681 agaggtaaga aaatctggaa taagaggcat tttttcaaag aaagatattg aattgactat
    16741 tggatgggaa aagaaagata atttagaaca aaaagattta aaacgagaga tagaacaact
    16801 aaagtcaatt ataaataata tggggtttga taataaaaat gataatgata tagataaaat
    16861 atgcaagaat ttactaaact tagatttaaa tcaagaaatt gtagaattta taaaagctga
    16921 tttacaagaa atgaagttca atggaattga tacaagtaaa aatttagtag aaagtctaaa
    16981 gaaaaagatt aaaatagaaa atcaagcaat aaatggtaaa attgctttag ttggaccacc
    17041 aggagtaggt aaaacgacta caatagcaaa gttggctgcc aaattagttt ttgaggaaaa
    17101 taaaaaagta ggagtaataa ctatagatac atacaggatt ggtgctgtag aacagttaaa
    17161 gatatatact gatataatga atattccttt taaaggggta ataagccctg atgagatgga
    17221 attggcactt gatgcgatga aagattgtga tgttgtatta atagatacaa caggtagagg
    17281 ctataaaaac tctatgcaaa tattagaaat caaaaatctt atagataaag ctgaaactga
    17341 taatatacat ttagttttga actgtacaac aagagaaagt gatacaaaag caattataga
    17401 ttcatataga aatgtgaatt ttaaaagctt aataattaca aagttagatg aaacaataac
    17461 atatggctca atatttaaca taatgaatta tgcacaaaaa ccaatatcat atattacaac
    17521 aggacaaaat gttcctgatg atattataaa acctaatgaa gataagatta taagattact
    17581 attaggagtt gaaagtatat gatagaccaa gctcaaattt tgagaaaaat ggcaatggaa
    17641 aaaagaggtt ctgatgagat tatagtagag aatgagaata tgcctaagat aattacaata
    17701 gcttctggta aaggcggcgt tggaaagagt aatctcgcta caaatttgtc tatatgctta
    17761 accaaacttg ataaaaaagt tcttatatta gatgcagata taggaatgtc taatatagat
    17821 ataataatgg gggtaaatgt aaaagggact ataattgatg taattaatgg cgaaaaaaat
    17881 atagaagata taatttcaca gactaagtat ggtgtaaatg taatatctgg aggttctgcc
    17941 cttaatcata tagaagactt tacagaagct caaagaaata agtttataca tagcatagaa
    18001 cagattcaca atgtggattt tataataatt gatacaggag ctggtatgag caaaagccta
    18061 ttgtcattta tatactgtag tactgaattt ttcttaataa caactccaga accgacatca
    18121 ttgactgacg catatagttt attaaaagct ataagtaatt ttggcataaa gaaaagtgca
    18181 aatataataa tcaatagggt aattgattta gaagaagcga aatctacata caaaagaata
    18241 aaaatggttg tggacaaatt tctaaacctt aatctggaat tgtatggata tataatagat
    18301 gacaaaaaag taagtttatg tgtaaaaaaa cagataccat tcattttaga atatcctaca
    18361 agtattgcat caaaatgtat tatagccata gcaaagagaa tggtaagtca gtcaagagaa
    18421 gatattgttg ataataaaag ttttctaaga aagatgttta gtatatttaa aagctaagga
    18481 ggcgtagtta atgaatagag aagaattaat aaaagagaat atgcctcttg taaagagtat
    18541 agcaagaaaa ttttttatac cagggaaagg ttttgaatat caagacttag taaatagtgg
    18601 agtcattgga ttgattgatg caataaataa atttgatgct gaaaaagggg caaagttttc
    18661 tagttactct tatataaaaa taaaatctgc tatattagat gaaattagaa atcaaagtcc
    18721 aatttctaaa cataatctaa ctaaagtaaa taagtataat agagtggtag aaaagttgca
    18781 atctaaatta ttaagagagc caacttcata tgaaattgca aaagaattaa aggtttcaga
    18841 aaaagaactt catgatatcg aaagtaatat agatatgtta aatattgtat cattaaacta
    18901 tgtaatcttt gaagatacaa atgaaactgt acaagatgta ataagtgata gagaagagga
    18961 agctccagaa aatataatag aagaagaaga aaagttagag attctatcca aagcaattag
    19021 taatttaaat gaaagagaaa aattaatact ttcactgtac tattatgaag atttaaatct
    19081 aaaggagata gggaaagtgc taggtgtttc agaatcaaga gtttcacaac tccacagaaa
    19141 atcaataaga aacttaagaa acaagataaa agaacttaaa tattctatat agatggtgat
    19201 tgataagatg atacaaatta tattaatagt agtgtctatt ttaataatag taactatagt
    19261 tataaattta cataaggaat caaaaaataa agataacaga tactttgata aaatatttga
    19321 gcaagaatat gaagcaaatg atagattaat aattgcagaa aaaagaagat tctttttaga
    19381 caaagtactt gctgaaataa gaagtaataa tcatataaaa gtttatgaaa attataacct
    19441 aataaaggct aatgaagaaa gaaaggaaaa catatttata gaatcaaaaa aggaagtaag
    19501 acaagaaaat gagttatctc ttaaagtgaa acatcttata agttcaggtt atgatgatgt
    19561 ggaaatatgt aagatgctag atattggaaa gggggaactg tcattaataa ggagttgtta
    19621 caaaatataa ggatattatt atgtgataaa aaagtattct taggaatagg tattggtata
    19681 ttaatatcaa ctttatgtat aatgcttttt agtaataatg atatgagtaa ggcagagata
    19741 gaaaaaaagg ccagagcgtt aggcatgaaa tatacaagtg agatgaaagt cttagaatag
    19801 atgatactgt ttagacattt acaaaaaagg aggtcataat gtgataagag gattatatac
    19861 agcagtttct gctatgataa ctaatcaaca aaagcaaaat gttgttgtca ataacctagc
    19921 taatatggat acaaatggat ataaaagtaa acaacttttg actaaaagtt ttgatgaatt
    19981 aaagttggag ggttacaata actacgcaaa tggaactaaa caaaagcaga ttataggcga
    20041 tataagtcca ggtatttcta tggatgaaac tatcacaaat tttaatcaag gacctataaa
    20101 aactactgat aataaaatgg atgtagctat tcaaggtaag ggattttttc tagttagtga
    20161 tgcagctgga aatcaatttt acactagaaa tgggaacttc agaacagaca atcaaggtga
    20221 ccttataaca agtgaagggt attatgtact tgggacgaat actgctactg gtgctgtagg
    20281 tcatataaat gtaggaaatc aaaaatttga agtggcaaaa gataatacaa tatcactaga
    20341 cggaaatgct atgtataaat ttaatattgt agattttaat aactacaata acttaaaaaa
    20401 agaaggagat aacttatatt ctggaggagg agctatgaaa gtaaataatg cacttacaat
    20461 acaaagtgcg atagagtcct ctaatgtaga tatggtatct gaaattaata atatgatgac
    20521 aatatcaaga gaatatgagg caaatcaaaa gataattcag gctatggatt ctaaattagc
    20581 aaaaatagca agtgagattg gttcagtaag ataggtaata ggaggtgaat ttttatgtac
    20641 acaatgtttt atactagtaa aacagcttta tcagcaaatc aaagtaaact agatataata
    20701 tctaacaata tagcaaattc tgattcaaca ggatataaaa aaataaatat ggaatattct
    20761 gatttagtgg atgaagtact aaatagacca tcatatccta caaatggcaa tgatatatcg
    20821 actggaacag gagctaaggc aacagctccc atgagaaatt atgaacaagg tgcattggct
    20881 cctacagata gtaagagtaa tttggcaata gatggagaag gattctttag agtgataaga
    20941 aaagatggta catatgcata tacaagaaat ggtggattta atgtagatgc tttaggtaag
    21001 attgtagatg ataatggaaa tatattagat gtccaatttg atgcaggata taactataat
    21061 aatacaaata tagatagtac aaacctgaca gtttcaagaa atggagagct attcgtagat
    21121 aataaaaaaa taggaaaaat caacttatat caacctatag gaactcaaaa ttttatatct
    21181 gaaggagata gtttattcgt cgcaactgat gcagcacaga ttaaacaagt tgagaaagtc
    21241 gatatagttc aaggctatag agagaggtca aatgtttctt tacaagaaga atttgttgac
    21301 ttaatagcca ctcaaagagc atttcaaatg aactcaaagg gaataaagac agcagatgaa
    21361 atgtggcaaa tagcgaataa tttaagagct aaataattat gtaaggtgag gaatattcat
    21421 gaatgatatg aaagagtacg attttggcaa acctcagagt tattcaagag aaaatttaac
    21481 ttgtttaaat tttttattag ctgaattttg taaaaaatat aaaaactata taatttatga
    21541 acttaagtgt aattctagta tggttgttga tagtatagac caagtaaatt atcagacttt
    21601 tttagataga gtaacttctt cgtgcgtagt agtacaaaat gcaatggaac ctgttatgaa
    21661 aaacttttct tttagaatag atagaagtgt agctgatatg tggattgata taacttctgg
    21721 aggaactgga gttgtaaagg ataatgatag agagcttaca gaacttgata aaaaagtgct
    21781 tttacacctt atggatgatt tagtaagaaa tatgcactta tttgagggtt ttgaaaatat
    21841 acaagtattg gatacacata cacatattaa tttaccacaa ttatgttctc caactgcacc
    21901 agtatgcgtt gtagatttaa aagtgctaaa tgataatgag tatattggaa gtgtttctct
    21961 ttgttttcca tacaatggtt tagaagctct gttagaaagt atatctgtta tagaattttt
    22021 tgataatgat ataggagatg attcagagga atttaccaaa aaaatatata atagtgtatc
    22081 taatatagaa ttatcggtta ttgcagagat aggaaaagta tcaattaatg ttgaagactt
    22141 acttaaacta gaagttggag atgtaatagt aacaaataaa aaaataaatg attatataga
    22201 tatatttgta gaaaattcaa aatcatatac agcaacacca ggatttatat caagtaaaaa
    22261 aggtgtaaaa ataaatgatg ctgttggaaa agaggtgtag ttatggacaa tagtaatctt
    22321 atttcagatg aagaagcaaa gatgctatta aatgataaag ttgaaagtga agttgatgga
    22381 gatgatgagt tagccttaga tgtgtctgaa gttaatcgaa ataatataca aaggatactt
    22441 gaccttaagt tagaattatc tgtggttata ggaagaacta agatgactct aagagatatt
    22501 ttaaacctac ataaaggttc tcttattgaa ctggatactt tagcagacca agatgtggaa
    22561 atactaatag ataataaagt gttggcttat ggaaaagtag tggtggtaga tttaaatttt
    22621 ggtgtaaaga taacaagtat agtaagtgaa gaagatatgg taaaatctct tgtttagaga
    22681 taacatcaat taatacttaa ggcatgaggt ggtacttatt ttaaacttaa atgatataaa
    22741 aaaagatata ttaaaactca ataaaaaaga ctcatgttct ctatataatg aatctattaa
    22801 taatataaat aataattgta tatcgagtgg attgaaattt ttaaaacaag caagagattt
    22861 aaacccagat gatgttgata tattaaatct cacaggtctt gtaaatttac taaaatgtaa
    22921 ttttgatgag gctgtagaaa gtttctataa gagttcactt tgtgaaaaaa ctgctctgtg
    22981 tgaaaagtat ataaacatgc ttacatctga ggagttttta attttctttg aaaggtacaa
    23041 tcaactcata aggttaataa acagaggaat gtataaagaa gctatacaag ggtttaaact
    23101 tataactgag caatactgtg atttaataga accttatgaa ttgcttgcct tactgtatga
    23161 caaggaagaa gaatatataa aatttgatga atgtttagaa gtattgaaga ttattgataa
    23221 ggaaaattat ttattaaatc aagaaaataa cacattagat atgtataata gtgctaatat
    23281 acaaactaaa agtaatataa aagtagatga ttataagaat gagttcttac ataaaagtag
    23341 tagtaaccat aaaattaatg attataatga tgtaactaaa gaaaagagtt caagcaaaaa
    23401 ccagaaaagt tcagattcta ataagaaaaa aagtattaaa aaaaataaat acctatatat
    23461 agccataata ttaggaatat tattagtagg acaaagtata tatagttctt ataagatgaa
    23521 taatttaact gataaaatat ctaaaaataa tgaaaccaaa aatgaagagt caatagattc
    23581 taacttgact aagaaagaaa ataatataga agaaaatgaa gatatgagta aggcttctaa
    23641 tataaataag agtaataaaa acattaataa agaggatgat gtaaaatcta aaaaaaatac
    23701 tgtagaaaat gaagatgata aagattctga cttatttaca gaggatgaat tgatgtctaa
    23761 ggcgaatact ttaaaatcag aaaagaaaac taaatcttct atacaactat ataagaaagt
    23821 tgcagatata ggtgaaaata aagggaatac atctgaagca acttatcaag ttgctattct
    23881 aagtgaaaag ttaaaagact ataaaactgc tgaagaatat tataaaatgt atgtagaaaa
    23941 ttactcagaa aaagatgctt attttgatga atcttattat aatttaggta tgatgtacta
    24001 taacaatggt gatttaaaaa attctaaatt gacattaaag aagttggtta ataaggtacc
    24061 aaatagtatg tataataatt caaaagtaaa agaaatttta aaagaagaat agttaaatta
    24121 gataagaaaa ataaattatt ttattttaaa ataatatgat tataatatat tgacatacca
    24181 ttttaaaaaa tttaaaatat taattataaa atgaggaggg ataaaaaatg gaatttgaaa
    24241 aagggagtat ttcaattcat acagaaaata tttttcctat aattaaaaaa tggttatact
    24301 ctgataaaga tatatttata agagagctta taagtaatgg atgtgatgca gtaagtaaac
    24361 ataaaagact tgtatcacta ggagaaatat cagaaaataa gtcatcagat tataaaatta
    24421 cagtatctgt aaataaagga gaaggtacac ttaaatttat tgacaatgga attggtatga
    24481 ctgaagaaga gataaaaaaa tacataaatc aagttgcttt ttcaggtgct gaagatttct
    24541 ttaataaata taaggataag atggaagagt caaatgacat aataggtcat tttggattag
    24601 gattttattc tgcatttatg gtgtctaaaa aggtacaaat agataccctt tcatatacag
    24661 aaggtgcaac tcctgtaaga tggatatctg aaggtggtac agaatacgaa atatctgaat
    24721 cagatactag aaatgataga ggaactacta taacactatt tattgatgat gatagtaaag
    24781 aatttttaga tgaatttact gtaagaggaa taataaataa atactgttct ttcttaccag
    24841 tagaaatata tttagaagat gttgaaagat tagaaagaga agctaaagaa gcagaagaaa
    24901 aagctaaaaa acaaaaggaa gatggaaaag aagagattgt agatgcaaaa gtaattgagc
    24961 ctttaaacga cactaatcct ctatggttaa aatctccaaa agactgtact gatgaggagt
    25021 ataaagagtt ttatagaaag gtattcaatg tatttgatga acctttattt tggatacatt
    25081 taaatgtaga ttatcctttc aatttaaagg gtatacttta cttccctaaa ttaaaaaatg
    25141 agtttgaact gactgaagga aaagtaaaat tatacaacaa tcaagtgttt gtagctgaca
    25201 atataaaaga agttatacca gaatttttac ttttattaaa aggtgttata gactgcccag
    25261 acttaccatt aaatgtatca agaagtttct tacaaaatga cagagacgta agcaaaattt
    25321 ctaagcatat aattaaaaaa gtagctgata agttaaaatc tctttgtaaa aatgaaagag
    25381 aagaatacaa taagttttgg gatgatatac aaatatttat taaatatggt tgcttaaaag
    25441 atgaaagttt ctatgagaag gtaaaagaat gtatattatt caaaactatt gatgatgagt
    25501 atataacatt acaagattat ttagaaaaat gtaaggacaa acatgaaaat aaggtatttt
    25561 atgtaagtga taaggaacag caatctcaat acattaaact atttaaagaa tacgacttaa
    25621 gtgctgttgt tttaaactca tcaatagata ctcattttat ttcatttatg gaatataaag
    25681 agaatggagt taagtttaat agaatagatg cagacctttc tgatgtatta aaagataaaa
    25741 atgaaaataa agatagtgaa gaaaacaaag aagagatagc taagatagaa ggtctgttta
    25801 aagaagctgt tggagaaaga gtgaagcatt attctgtaga aggattgaaa aatgaagaca
    25861 ctccagctat ggtgttagta tcagaacaat ctattagaat ggcagaaatg cagtcaagat
    25921 ttgctggaat ggatttagga atgaattttg aggaagaaaa aactcttgta ataaatgaga
    25981 acagtccaat aatcaaaaaa ttggtttctc ttaaagatga tgaggaaaag aaagataaga
    26041 ttacgcttat ttgtaaccag atagcagatt tagctttact ttctaataaa gaattaaagc
    26101 cagatgagtt agattcattt gtacaaagaa gtaataagct tatgagtctt ttcatagaat
    26161 tataagattg aatttgaata cattgaagca aatttataaa aagtattcaa tatcagtgat
    26221 gataaagaag tgtctcaaaa tgaacttttc agttcatggg gcgctttttt tatgtgaaat
    26281 taaatttatt ttaatcattt caacaatgaa aaatatattt atctctattt tgagacagac
    26341 cattctttat ttttttatct ttggttaatt tttaaatatt taagtaggtt ctaataaaaa
    26401 actctattta gttaaatata gttaacttga tatataataa gataatacat ataattaagt
    26461 aaaagattgt tttttagttt atgtttaaat acataattac actttgttaa aaacataaaa
    26521 ttattactaa agtagaaata ggaggtttta gcatgaattt taattataat ttgccagtaa
    26581 atatcttatt tgggagaggc agaattaatg atgtgggaaa agaagtatct aaatatggca
    26641 aaaaagtatt gatagtaaca ggtaaaaata gtacaaagaa aactggatta cttgataaaa
    26701 caatagattt actaaaagac tctaaaatag aatatgaagt ttttgataga gtagaacaaa
    26761 atcctcttat aactactata tattcaggag tagaaattat aaaatcaact ggatgtgatt
    26821 gtgttttagg gcttggtggt ggaagtatta tggatgctgc caagagtata gctttttctt
    26881 ataaaaattc aggagattta aatgattata tatttggaat tagacaaggt gaagaagctt
    26941 tacctataat cttagttcca acaacatcag gtacaggtag tgaaggaaat tgttttgcag
    27001 tacttacaaa ttcagaaact aaagataaaa aatctataaa gaagaatagt atgtatgcaa
    27061 aagcatcaat aattgaccca gaacttatgg ttacaatgcc aaaacatata attgcatcag
    27121 ttggatttga tgcacttgcc cataatatgg aagcatatct ttctaatgga agaaaccctc
    27181 ttgcagatgt acaagctatt tatgggattg aacttatatc agaaaaccta ataaaggtat
    27241 ataatgatgt taatgattta gaagcatggg aaaagataac acttgcaagt actattggag
    27301 gaatgacgat tggtacagcg ggtacagcct tgccacatgg aatggagcat cctgtaagtg
    27361 gtcttagaga tgtaatgcat ggaaaaggac ttgcagcttt gactcctact attatagaaa
    27421 gttcatggga tagtgatatt gaaagatttg gtaatatttc aaaggtatta ggaggaactt
    27481 gtgcagaaga ttgttcagat gcaattagaa atttccttaa aaaaatagat ttagatataa
    27541 agctaagtga tttaggtatt gaaaaatcag atatagattg gttaactgat aattgtatga
    27601 aaatatcagt tgcaaatatt aagagacatc caaagtattt taataaggaa caaataaagg
    27661 aaatgtacca taagtcttta taatttgact ttacatattt agttttatat aaaaattata
    27721 aattaataat aaaaaattat aagtaattaa tagtaaaaaa attgtgtata gaagttatat
    27781 atataataaa tttgtcttaa gcttttattt tactttctaa taaataaagg agtttaaaaa
    27841 agggaagatt aattaagtaa tattttataa aattcttcga tttaaaaact ttaagtagag
    27901 gtgaaataaa tgtttaatcc caaaagaata atattcgaaa aaggaacttt agacactgat
    27961 gtgggtaaaa aaatatacaa taaatttaga gataataaag atgtagagat aattaatata
    28021 tcatcaaata ggattaaaga acatattcca ggtgaaaatc tatatgaaca atatagaagt
    28081 gggaagcaga ctatggtagt tgggattaga aaaagtctga agtttcaaac atgtaaacct
    28141 tctgctaatt atcaactacc acttgtcagt ggatgtatgg gaagatgtga gtattgttat
    28201 ttgaacacac aattaggtga taaaccattt ataagggtat ttgtaaatgt agatgaaata
    28261 cttgaacaga caaaaaaata tattgaagat agaaagccac aggtaaccat atttgaaggg
    28321 gcagctacat cagacccagt tccagtagag ccatatacta attctttgaa gaaaacaata
    28381 gagttttttg gaagagagga aaaaggaaga tttagatttg ttactaaata cagtgatgtg
    28441 gatagcttat tggatgctga acataataat catacagaaa tacgatttag tatcaatact
    28501 cctagaatta taaatgaaca tgagcattat acagcctctg caaataaaag aatagagtca
    28561 gctgtaaaac ttgctagggc tggttatcag attggtttca taatagcacc tgtgttttta
    28621 tatgaaggtt ggaaagaaga gtataaaaat ctgattataa gtataaaaga agctttacca
    28681 aaggattttg ataaaaaaat tatatttgaa gttatatctc atagatatac aactaaagca
    28741 aaaaatagaa tactagagat ttatccagat acactattgc caatggaaaa tgaagataga
    28801 aagtttaaat acggtcaatt tggatatggt aagtacgtgt atgataaaga acaattagat
    28861 gatatgaaag agttctttct taaggaaata aatggagtat ttaaaaatag tttaataaaa
    28921 tatataatat agcaaatctt acctaaagat ttgctatatt ttatttttat atattttatg
    28981 tttataataa agcaatgaga tttagcacta tcatataaga aaattaaaaa gggcactatc
    29041 tatcaagaac attttttcta gttttgatac tataaaggat aagagtaaca aaaatatcag
    29101 gaggagttat agtatgcatg catgggagtc tattcaaatt acattggatt taatagaagc
    29161 aaatttatca gaagaaatat caattaatga attagcaaat aaagcaaatt tatcaccatt
    29221 ttactatcaa agactattta aaagattggt aaataagact gtaatggagt atataaagct
    29281 tcgtcgtctt gcaagagctt ctgaatactt agcagaacat aagaatcgta tacttgatgt
    29341 agctttaaat tttggatttg ctagtcatga gacatttaca cgttccttta aaaaagcata
    29401 tggattaact ccagaaaaat atagagcaaa tccagtaaaa ctcaatcact ttattaagcc
    29461 agaattaatt ttaaattatg ctatggttga tgaaggtgtt ccactgattg cagatgatat
    29521 agtgattgaa gtcaatcgta agacattgag taatccaagg acatttgttg gaatagaaat
    29581 agaagtacct atttgccagt tggtgggcgg tgaaacaact ggaattgcaa tagtagagga
    29641 attatggatg aaacttggaa gtcaaagaca taatatacca aatcaaattc caggtggaaa
    29701 tgaatttgct gctctttata tgggaaatgc aaaagaagga aattgtatgt atatggcagg
    29761 tgtagaggta gagtcaggta catcagtaga aggatatagt acttttgagt tgccagctaa
    29821 agaatattta gtatgtggat ttgaggcaga aaactttaat gagttagtta attcagctgt
    29881 ttttaaagca gataaattta tggaaagatg gatgaagaaa cataatttaa caactactga
    29941 ttttgcaatt gagatgtatt accccactac acctgaagca gcatatctgg aacattggat
    30001 tgttccggtt cctattgaac aatagaaagg atgatgaata tgaattggca agatgcgttt
    30061 tttcaagata ctaaacctac atttgagcaa attaatgaat ttattaataa tccattatgg
    30121 gacaagttaa ataaaacttt ggtttctact tataatgtag aacctaagct tgaatacagt
    30181 aaatgttcaa tgcaaagagg ttggaatgta aaatataaaa aaagaggtaa atcactatgt
    30241 actttatatc cacaagaagg ttattttaaa gctttggtta ttgtaagtga aagcaataga
    30301 gtagaggttg atttatttat taatacttgt tgtgattata tcaaaaaaat atataatgag
    30361 gtaaactttt ttaatggaag taaatggcta atgattcaag tagatagttt attggtatta
    30421 aacgatatgc ttgaactaat taaatttaga gcttaacata tagaacagga tgaatatata
    30481 agtcatcctg tttttatatt caatcagata aattaatata tagtctataa tatgaataat
    30541 ttagtaaatt taaatttact ttatctttct acattctttg caactaagtc cacatgtgtt
    30601 tttttcttgc atattagtag caccccaatt agcaaattgt tgtaaaatag gcacaatact
    30661 tttaccttta cttgttaatg tatattcaac ttttggagga atttcattat attgtttacg
    30721 atgaacaata ccagtagact ctaaatcttt taatgattga gctagcatag tatttgtaat
    30781 gccatccaat tttcttttta tgctactata tctaattgga ttttcaactg ctaacataca
    30841 tataataggt aatttccact taccaccgac aatgtctaat gcataacgga ttggacaggt
    30901 taattcttca acgtttttag ttatattttt ttcagtattt atactcattt taattactcc
    30961 ttagttatag tagtatcttt tttatacttt atcagaaaaa actgcatact tgttttttct
    31021 gtattttata ttaaaattat atcaaaggat aatccttaat acaagtattt aaaaggggtg
    31081 taagaattat gaaagcatta gaatttttga acaaggcacg agtttattat ttagcaacag
    31141 ttgatgagaa caatcaagca catgtgcgtc ctttaggatt tgttatggaa tacaatggta
    31201 aacttacatt ttgcacatct aatcaaaagg atatgtttaa gcaacttatt tttaatccaa
    31261 aggttgaact ttgttgtata gaccaaaacc ttaatactct acgtatttta ggtgaagcag
    31321 tatttgttac atcaaaagaa acacaagcta aggcacttga aataatgcca agtttaggtc
    31381 agatgtactc tgtaggagat ggtaagtttg aaatattctc tattgataag gcaaaagtta
    31441 gttgttgttc aatgagtggt aaaaagattg atgtagagct ttaattataa ttgaatcgac
    31501 atccatagtg aaaattaaga aaacgttatt actgaaatgt tgatagattt aatttaaatg
    31561 caataaaaag atttttaaat aatgaatatg taaaaatttt tagttattag atgtgtattt
    31621 caataatgat atactatttt gaataattac atactagaaa taagataatt aaaatttact
    31681 ttataatggg atagatttct tttaaaaaat attttaacta taattgattt ttatttaatc
    31741 tgaaaaaaat attatgacta taaaaaattg ctacttatta tttggatatt tttatatata
    31801 aataataagt agcatgactt tatacattaa taatgataga atataaatta atagataaga
    31861 tatgatttta aaaggagtga tataagatga gtaaatatag ttctttatgg gaatatgtta
    31921 agaaaaacaa tagtcaatcg tttaaattaa cttttgaaga aataaaagat attgctggga
    31981 tagagataga ccattcgttt ttgaaatata aaaaggaatt aaatgaatat ggatatcaag
    32041 taggaaaaat ctctttaaaa gagaagactg ttattttcga taaaattggt taagacataa
    32101 ataattacat ataataaaat aatttgtatt gatgattatt acaaataaaa aagggcgtag
    32161 cactattttt tagtgtcaca gccccttttt tgtttataat tatgtacatt tcacattatc
    32221 acgatataag catttttctt atgagaaaat taattagaat tatttttatt catatttaaa
    32281 ttatttaaat tgttttccca gttttttatc tcctttttaa cttcttcact taaagtatca
    32341 cttgaagata ttttctttaa tttatcattc aaagatttct tttgttcctc tgaaaggtct
    32401 gtttttattg taggatcaat aatatctatt acatctttta catcaaaatc aacaaatttg
    32461 cacagttctt ctagatgatc aattagaaaa tctagacctt catatgacca ttctttataa
    32521 tcttttccgt tttttaatag aaactcattc acttggtaat tctgatttgc taattcaaag
    32581 aatgaacctg aaggatccat tatatcattt atagagtttt catagttaac aatattaatt
    32641 tgtcttatta atttctcttt ttctgttaag ttgctataat tcttgttaac aaaagctact
    32701 aatccaaatt caaagtttaa aattgaatct ttacgttttt ttactgaatc ttctccaaat
    32761 tccctaagac cttctaaatt tttatcaatg gctttatctc taatctctat taattgttct
    32821 atacttaaac cttctaagtt tttaggtact atttcttcat tattcttagt aacttctttc
    32881 aatttattgt tagtattttt attattgaaa ataaatatag cag
//
LOCUS       DAGSWI010000034        32190 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.034, whole genome shotgun sequence.
ACCESSION   DAGSWI010000034 DAGSWI010000000
VERSION     DAGSWI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.034"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(78..1292)
                     /gene="dpaL"
                     /locus_tag="MJF80_001445"
     CDS             complement(78..1292)
                     /gene="dpaL"
                     /locus_tag="MJF80_001445"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBZ0280117.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(1915..2454)
                     /locus_tag="MJF80_001446"
                     /pseudo
     CDS             complement(1915..2454)
                     /locus_tag="MJF80_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(2531..4336)
                     /locus_tag="MJF80_001447"
     CDS             complement(2531..4336)
                     /locus_tag="MJF80_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0280118.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(4732..5109)
                     /locus_tag="MJF80_001448"
     CDS             complement(4732..5109)
                     /locus_tag="MJF80_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBZ0280119.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6014..6499
                     /locus_tag="MJF80_001449"
     CDS             6014..6499
                     /locus_tag="MJF80_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280120.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(6687..7226)
                     /locus_tag="MJF80_001450"
     CDS             complement(6687..7226)
                     /locus_tag="MJF80_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBZ0280121.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(7219..7632)
                     /locus_tag="MJF80_001451"
     CDS             complement(7219..7632)
                     /locus_tag="MJF80_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0280122.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(7668..8789)
                     /locus_tag="MJF80_001452"
     CDS             complement(7668..8789)
                     /locus_tag="MJF80_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBZ0280123.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            9388..11040
                     /locus_tag="MJF80_001453"
     CDS             9388..11040
                     /locus_tag="MJF80_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBZ0280124.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTNQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            11339..12088
                     /locus_tag="MJF80_001454"
     CDS             11339..12088
                     /locus_tag="MJF80_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280125.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            12101..14128
                     /locus_tag="MJF80_001455"
     CDS             12101..14128
                     /locus_tag="MJF80_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280126.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYDNIQIKKIIKKQVLPFFNIPFLLGILDCIFAT
                     LVYKTALMQNLLGNSFSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKN"
     gene            14272..15855
                     /locus_tag="MJF80_001456"
     CDS             14272..15855
                     /locus_tag="MJF80_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0280127.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNISDNGVANTSFKTPLDITKITFDTTKHKGIISIKEQVENLGGTISVSDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            15827..16447
                     /locus_tag="MJF80_001457"
     CDS             15827..16447
                     /locus_tag="MJF80_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0280128.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGGTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(16642..17568)
                     /locus_tag="MJF80_001458"
     CDS             complement(16642..17568)
                     /locus_tag="MJF80_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBZ0280129.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRDELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNNESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            18178..19098
                     /locus_tag="MJF80_001459"
     CDS             18178..19098
                     /locus_tag="MJF80_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBZ0280130.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            19184..21604
                     /locus_tag="MJF80_001460"
     CDS             19184..21604
                     /locus_tag="MJF80_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBZ0280131.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPISDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSNKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLSAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVYFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPNLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKALKENP
                     KSITGRYL"
     gene            21604..22626
                     /locus_tag="MJF80_001461"
     CDS             21604..22626
                     /locus_tag="MJF80_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBZ0280132.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            22760..23506
                     /locus_tag="MJF80_001462"
     CDS             22760..23506
                     /locus_tag="MJF80_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0280133.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            23592..24842
                     /gene="cme"
                     /locus_tag="MJF80_001463"
     CDS             23592..24842
                     /gene="cme"
                     /locus_tag="MJF80_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBZ0280134.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(25359..26372)
                     /locus_tag="MJF80_001464"
     CDS             complement(25359..26372)
                     /locus_tag="MJF80_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280135.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKYSILKGFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(26375..27403)
                     /locus_tag="MJF80_001465"
     CDS             complement(26375..27403)
                     /locus_tag="MJF80_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280136.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            complement(27403..28140)
                     /locus_tag="MJF80_001466"
                     /pseudo
     CDS             complement(27403..28140)
                     /locus_tag="MJF80_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(28208..29917)
                     /locus_tag="MJF80_001467"
     CDS             complement(28208..29917)
                     /locus_tag="MJF80_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBZ0280137.1"
                     /translation="MPRRRKSQESRLTNNRTIWKVAVYIRLSREDGNDESLSVINQKK
                     ILKEYLEEMFEDEYVLVDFYVDDGLTGTDDERESFQRMIKDVKQGKVNCIICKTLARA
                     FRNYSDQGYFLEEFLPLHRTRFICLGSPKFDTYLNPEVITDGLDVPITGLMNDRYAAR
                     ASADIRRTFNTKRRNGEFIGAFAPYGYKKSPDNKNKLIIDEEAAQVIRDIFRWRVEEG
                     INKLGIVRRLNELGTLTPTKYKHSKGLMLKNPKLDKQDGMWSMNTISTILKNRMYIGD
                     MVQGRQRVISYKVHKTICTPENEWFIVENTHEAIIDKDTYLKAQELDQRDIRMSPKGR
                     NVYLFSGFLRCADCKRSMTKKTNKNKLNDGTVKEYNYYICSTYALKNKSKCSRHSIKL
                     EILEEAVLKAIQIQIAMVENMSEVISEINKQAIIVNQSSRLKKQLNEKQKELEKIICV
                     TDNLYMDWKSEEISRNEYARMKSRFEIKAEEIRRIISNIEAEIQSSSKGVGDNDPYLK
                     TFLKYKNIKELNRGILIELIDAIYIHENNEITIQFKFADQYKRLKEFIKDNHFSKKLK
                     YNN"
     gene            complement(29974..30216)
                     /locus_tag="MJF80_001468"
     CDS             complement(29974..30216)
                     /locus_tag="MJF80_001468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280138.1"
                     /translation="MSVNIVKKDNEKLNKKYIDIDKVINKQKDLEDEDLERCLKEMKS
                     PYCFICGDVVVRLKFSDSENTLDLCLISYFKNLKNC"
     gene            complement(30807..31046)
                     /locus_tag="MJF80_001469"
     CDS             complement(30807..31046)
                     /locus_tag="MJF80_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0280139.1"
                     /translation="MSLVHKNNKLLPYDIIYAAAKGDIIAIGIVLKNYEAYIVKLCTK
                     TLYDKRGNAYKCLDLEKKNLLELKLIYKILKFKFV"
     gene            complement(31030..31467)
                     /locus_tag="MJF80_001470"
     CDS             complement(31030..31467)
                     /locus_tag="MJF80_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBZ0280140.1"
                     /translation="MKNSSINKESTIHKFDSFCKKILKNESIDIERKNRYLRQVQILF
                     CELSEEQKNSLFSNDLYLSDYYSFNVNGFKIHIVNEALGEAIKLLPELRQKIILLSYF
                     IGYNDKEISGILNVSQDTVWYQRNVAIKTLRKILGVKKNEFGT"
BASE COUNT        11294 a         5016 c         4019 g        11861 t
ORIGIN      
        1 aagttttcac ttatttaagt ttttattttc cttaaatttt tcatttattt gttaagattt
       61 tcttttcctt aagtctttta tttactttgg cattcaccat tccaaactat atctctatat
      121 ttatcagggt cagtatctcc ttctgtactt attaataata ctttagagtt ctcatctagt
      181 ttcaaagctt ctcttaagtc ttttaaatct tcactttcaa ggattgaaac taatgctcca
      241 gttgtaactg ctccagattc tccagagatt actttctcat ctcctcttag agggtttcct
      301 aatattctca tacctttagc agatacccag tctggtgctg aaacgaatgc tgatgaataa
      361 ttttttaaca cttcaaaacc tatagtgttt ggttctccac aagcaagccc tgccattata
      421 gttggcatat ctcctccaac aaatcttggt tttccatctc cagcttctgc tgatttatag
      481 taacaatcag cctcatcagc ctctactact actgttattg ggcattcatc atatattgaa
      541 gcaacatacc cttgaactgc accagcaagt gaaccaactc ctgcttgaac aaatacgtgt
      601 gttggtctat ctactccata ttcttttaat tgttctatag cttctttagc catagttcca
      661 tatccttgca ttatccatgc tggtatttct tcatatccgt cccacgctgt atcttgaacc
      721 attactccat ggtcatcagc ttctgcataa tcattagcta atcttactgc atcatcatag
      781 tttaagtctg ttatactagc atctgcacct tctcctttga ttttatttaa tctaaattca
      841 gaagaacctt ttggcattaa tacaacagat ttttgaccta atttattagc tgtccaagca
      901 actcctctac catgatttcc atctgtagct gtaaagaaag ttatttctcc taatttttct
      961 tttatttctt ttgaagttaa tttatcatac cctaactcag atatatctgt atctaatctt
     1021 tgagctaagt atctacccat agagtaagaa ccacctaaaa ctttaaatgc gtttaaacca
     1081 aatctataag attcgtcttt aacatatatt ccgcttacgc ctagtgattt tgctaagtta
     1141 tctaatttaa ctaaaggagt tttttcatat tgtggaaagc tttcatgaaa agctttagcc
     1201 tttgcaatct cttcatcttt taaaaattca attccttttt ctttttcctc tgatttaggt
     1261 aagttattta atttccattt tatttctctc atttatttta tattcccctt tcaaattttt
     1321 cggtgtcatt ttaatttttt atctttgtgc cttatagcaa agcaattacc gtgccaatat
     1381 tatttattct aaaaacaatt tttttatgtt ggaattacat acttttataa aatttcttta
     1441 aagtttataa attttgagtg tgaaaaataa gatttataaa agaaaaaatt ctcatattgg
     1501 gaaattttta tcaatacgag aattatcaat tcgagaaatc gtttatttat cttaattctt
     1561 cctttattat gctcttattt tttatatgtc tattgttttg tcagatatat tactactttt
     1621 attttctata aatatatggc tataaccttc ttacgcataa actctataaa tttatatact
     1681 ttatattctt tattacccaa atattcttta ttacccaaag cctccaaaat ggcttaaatc
     1741 ttttcatagc ttcttacaga agtttacatg ggcgtccccc caataagcaa aagtgcaata
     1801 tgtgtaccat tgtgccctac ttgcgactat tgagctgtcg aaattcgcaa cttcgcattt
     1861 atctgatgaa atgcactata caaaatattc ataacatcca caacagagtt aaagttacct
     1921 attcatattc actgtaaaaa tttcttccac aggaaatgta attttaacca atcctattga
     1981 gacaaactca ccacttattt ttttgaactc acgcagtaat acatcacttg cccgaaaaaa
     2041 agcctcactt tcatcatctc cagtaagtgc tattgtgcaa tggggtaccc aacgattggg
     2101 gtggtaccat tcccaaccat ttgaatcaaa atcttgtagt acctcatata attctctctg
     2161 aaactgctac atactgctat tcattatagg tgatacaaac accgttttag tatcattaaa
     2221 catgcctact gaaccaatgt aagcaggtat taccttatgg ttttgtgcaa acccctttag
     2281 cttgtctata caacaagcct catccacatc attgaaacaa gccaaagtaa gatgaggtct
     2341 tgttttccat tctaaatact tcgtactaat tttttcatca gcgatttttt tagataaatc
     2401 aaaaagttgc ttttccgttt ttttatcata atacagctct atcgcatatt gcataataac
     2461 acctccacca taaagtaaaa atacctatct ataaaaacac acaaatacct atcattttaa
     2521 tattatatta ctactgttcc tcttccaatt ttctatatag tgtagccaaa cctatgccta
     2581 agtcttttgc tatcttcttc ttcgtctttg tatctcttcc atacttattc aacaacttgt
     2641 ttatgtaagt caactctaat tctttaattg aaagtatcct ttcttgattc tctactatcc
     2701 ctccaactat atcatcttca aatattatat ttacatcctt atttttataa atatttccat
     2761 taatattata atagtttaaa atatctaatg gaagcatatc tttatatatt attccatcat
     2821 ctccaaccaa attcatcata tattcaactg cattttccag ttcccttata tttcccggcc
     2881 aattatagtt aatcaacata ttcattacat tttcttctat tttatttaac tgaataccta
     2941 attctaaaga atatttagaa gcaaacttca tcattattgc ctcaatatca tcctttcttt
     3001 ccctaagagg tggcaaattt ataggaatta cattcaatct atagtataaa tcatccctaa
     3061 acttaccttc atttactaat ttcaataagt ttttatttgt agcagcaatc actcttatat
     3121 ctaaatctat aagtttattt gacccaattc tcgcaaattt tctctcttgt aaaactctaa
     3181 gtaactttgc ttgtaaaggc attgacaaat ctcctatttc atcaagaaat ataacacctt
     3241 catttgcaag ttcaaattta cctactttcc ctccactact agctccacta aatgcacctt
     3301 ttatatagcc aaatagctca ctttcaagga gattttctgg aatagcagca cagtttatcg
     3361 caataaatgg cttgttccat ctgctacctt cagcatgtat agccctagca attagctctt
     3421 tacccgtacc actttcccca gttataagta cagttgaatt tgactttgct aatttcttag
     3481 ttctctcttt tacttttaac attgcttctg aatttccaat tatagactct atatcactat
     3541 ttccccagcc actattcact tttccatgtc ctttatgatt gatatatgtc ttgtcaaata
     3601 ttattatttt gtcgtattga tttatatatg gatataccgg aattattttg gcatttatat
     3661 tataaatttt gttatcaata tcaatttcta gtaattcatg tccatttgag gaagattcaa
     3721 cagatactat atttactata ttttctatta tatttttatc tatatctaat ttttttagag
     3781 ctatattgtt tatataagat attttgctat ttatgtctag tattataacg cctttttcaa
     3841 cattttcaat gatttctatc atcatatcca tttttctact gctatttttt gacgcttcat
     3901 gttcttctat tttcatagaa attaaatcgc atatatgatt gacaaatttt aaataattat
     3961 ctatttttgc acttatttct acttttttag ttttatctgt gctaatagca ccaattactc
     4021 ctatagttcg attattatac tttattggaa ctgctattat tactttattt aggcatttca
     4081 tgtaatggga acattctata caaagctgat tatcacatat atccaatacg actaattcct
     4141 gacctgtctg tataacattg tcataaataa agccctcagt aacaacgctt tcatttatct
     4201 ttgatttata cagacctgta ccactaattc ttattaatct atcgtcaact atttcaatat
     4261 ctacatctaa cacactagct actgtttcag tgtattttat catttcttgt tgaaattctt
     4321 ttaatttact taacatagtt tcccccatta gaatttaatt taccaaatta tcagtttagc
     4381 tatattttaa catatagtca ctctttaaaa tatatataaa acattactta tttatatatt
     4441 tgcgttaaaa cagacaatat taatttataa aaagtttgtt ttatatagta accttaatta
     4501 taatatgcag acaataaaat aaatcacaat acaataataa aaaaaagctt tcctcatata
     4561 acaaatatat ttttgacata aaaatataca ttatatgaga aaagctcaat agttaaatat
     4621 ttagatagat ttttatatag acttttttaa tataaacttt tttaattgta gacttttttt
     4681 attagttttt aatttttatt agttttgtta ttggttttat tgttataata cttataatac
     4741 tgccattgct tcaatttcta aaactgcatc ttttggtaat tgagcaactt gtacgcatga
     4801 ccttgctggt ggattttctt taaaatatgt agcatatact tcattaatat cattaaaatc
     4861 ctttaaatct tttacgaata ctgtagtctt aaatatttta tccatacttg taccagcttg
     4921 ttctaatatt gcttttacat tatctaaaga agcttttgtt gcttcttttg ggtcagatac
     4981 taactcgcca gtcttcatat ctattggaag ttgacctgat gtagaaacta tgtttccaac
     5041 ttgaattgct tgagaatatg gtccaattgc tcctggagct ttctctgtag ctattactac
     5101 ttttgacatg acttcacctc ctattattaa gttatattta caggaaaata aaatttccta
     5161 aacaccaata ttaatacgaa cctttagatt aaatttcaaa tctaaaactt caaataatca
     5221 atacttagat gattatgttt atttgtgtct tgttaacttt tattaaagca attaacgtgc
     5281 caaactttat ttatatataa aattttgtga agtcattcaa aatagttact tctgaatttt
     5341 ttcgtaaaaa aacgctctaa aattactttt ttatgatgtc aatttttttc tcatatctag
     5401 aaatttacca acatgagaat tcttgttttg agaatttaca gattaaagtt attttttaac
     5461 cttaaattct gaacctccca tgactaaagt cacagggttc ctgcttcata gaattttgca
     5521 taatcaagtt attacatatg aaagaactat agttgacaat cccactatta taaataataa
     5581 aacctttacc cataccttat ttaccctagt tatcatagag cctattgaaa atataacagc
     5641 taaatataat aataaccaaa aaattatcaa tatctaccac tgttgctgaa aatccaaaaa
     5701 actacctttt gatagtatta cttaacaatc taattaatct cataataaat ttacaactta
     5761 taaataaatt gttatattgg ccgtatctca acaactttct cctttcctag ttttaaatct
     5821 tttatagaat tgtaccacgt ctaagtaaat tttcatacta ttaagtctta tttctaataa
     5881 atcattagct ctcattctat gcccaataat gtaagattgc agtaatttta ttgattttca
     5941 tagttttatt taatatactt atattaatta ttttcatatg aaattattat ttatataggg
     6001 ggattttttt aatatgagca aaagtgaatt aacagcagaa acaacagaag aaatgttaga
     6061 agtactaagt ggtaaagatt atgatattgc atgtcattta catgaacttg gtaaatcatt
     6121 agattgtaaa attgaaccaa aaacaggtgc tcgttcttac aaaatagtat attcaactaa
     6181 gaaaccaaaa cgtagcttat ttactattga atgtaatgaa aaaaaatgga gagttaaagc
     6241 aaatcttttt catctaaata catataaaga tgctgtggag gaatgctcta aaactattaa
     6301 agatagtata actaaaactc gtacttgtaa gaaatgtaat tcaaagtgta ttggaggttc
     6361 ttcgtttgaa ttagatggaa agtcttacct gacttgcata ggaagtggtc attattttgc
     6421 aaatatggaa gaaatggatt ggaaaaacct agaaaaatta attactaaag aaaataatat
     6481 tatgcaggaa tctgtatagt tttttaaggg taattattaa aatatatgag caacaacaga
     6541 tacttaaata ggcataaaaa aataaagggt ataataagga tatagaataa aaataaatga
     6601 tagcacaaag aggctgtctc aaaatacaga taagcctctt tttattgttg aaatgataag
     6661 aatatattca attttataca aatatactac gccaacttac ccatttcata tgcctgtttc
     6721 attgccttac ttcccttaat atcacctaca ttccaagcac ctacaccata tactatacct
     6781 ttttccttag aaccatttag acatgatgta aatcctctaa atccttctat tgttctttcc
     6841 atatcagcca cattttcatc tgctgctgta acgataaaat agaaatcttt atcacaaatt
     6901 tcagtatatc tagagcaagt tctatcaatc atagttttca tttgcgcatt catagtataa
     6961 aaataaactg gagtcgctaa aacaatcaca tctgccccaa tcattttttc aagaatttct
     7021 cccatgtcat ctgtctgtga acaatttgct tctttttcat aacaagcacc acatccagta
     7081 caatagttaa tatctttatc ttttaaaaat atcttctcca cttgattttt agattctaat
     7141 accccttgta taaactggtc acacaataaa tcagaatttc caccttttct aggactagct
     7201 gatactatta atacattttt actcataaga tactgcctcc attttcttat ttctactgct
     7261 ctttaatact ttttctttct caataatacc ttcttcatat ctatcaatct taaaatttaa
     7321 tcgttccaaa gttttttgca tttcttctat tctttttgct agttggtttc tctgctctat
     7381 taaaagttct tttctagctt tgatagtact gctcccttgt tgaaacatag ctacatactc
     7441 aattaatgtc tcaatagaaa gacctgcacc tctcatacac tttatgaaat taacccactc
     7501 acaatcttct tctgtaaaag acctaattcc acttttattt ctatttacag gtggtattaa
     7561 accaattcgt tcataatatc gtaaagtatc cgctgaaagc tcgtattttt tactaacttc
     7621 tgaaatcgtc atacatatct tctcctcttg atattgtata gattgaatta tttctcaaag
     7681 gtttctgcta cattctttaa agcatctata attttaatgt gttgaggaca gctttcttca
     7741 cattgtttac attcaataca atctgaggcc tttccataag ttttagtata attattatag
     7801 tagaccatct gtgttgaaaa tccagtatta agagcttgtt tttctgcatt gtacaacgca
     7861 aaatagtttg gaatagcgat attttttgga cagccttcta cacaatactg acaggcagta
     7921 caaggaacta aaattgattc gttgataatc tctactgctt catctataat attatattcc
     7981 tcttgtacga atggtttgaa attttgcata tatcctgtat tgtctaataa ttgttccata
     8041 tttgacatcc cacttaagac catcataaca ttatctttac ttgctgcaaa acgtattgcc
     8101 caagatgaaa ttgacatatc tggattgtag tcattcaata atttctctgc tttttctggt
     8161 atctttgcaa gtgtacctcc ttttactggc tccataacaa ttacaggctt attatgcttc
     8221 tctgccacct cgtagcattt tctagattgt atgctttcat tatcccaatc taaataatta
     8281 atctgtaatt gcacgaagtc tacttctgga tgctcactca agactttatc caataattct
     8341 gcactatcat gaaaagaaaa accaatattc tttatttttc cttctttttt cttttcttca
     8401 ataaacttaa agctatcaaa ttttttagct acttcataat gtgacacgcc tatattgtgt
     8461 aaaagatagt agtcaaaata gtctacacca catttttcta attgctcatt aaaaatggtt
     8521 tcttgctctt cttttttctt taaagccatt aatggaagtt ttgtagctat agtaaaactc
     8581 tctcttttgt gtctttttac tagagattct ctaagtgcta tctcactttt tcccatgtga
     8641 tacacatagg ctgtatcaaa atatgtaaat cccctttcta gaaaagtgtc taccatttta
     8701 tttaaatgtt ccatatcaat agttgtaaca tcattttcat cttttattgg taaacgcatt
     8761 aatccaaatc ctaatttttt tgcctccatt gttttacttc cccctttaaa acttgtttgt
     8821 tttttctctc tactttaact attcttacaa gtatataatt cttgtataat tatactttac
     8881 tacttggagt taacttcaag tcaatagatt tttttatttt taagacaaat attataaaaa
     8941 tacagttctt taatattaat ccattacatg tttctttcac cctccaattc tcaaacttca
     9001 caaactccaa catgtaaaga aatatatacc ttaagaaatt cagtccatta atattactac
     9061 aaatactttt tagatatgtt taaaaagaaa tttcacctaa aatatttata aaagatttag
     9121 aaataggtct tgtgtctatt ttaaaatata taaaagagtg gtaaagactt atttataaga
     9181 taaaaaatga atagtgtata ccctttttaa aaagtttttt cctttttata tttgtgatac
     9241 aatgttaaat ataaaaagga aaattttgta ttaaagaaat cttatttgtt gattttgtaa
     9301 cttcaaactc agcatcaatt tgattttaag attatatttt attaagtatg ctaaataaca
     9361 aatttttaaa gattgtgagg taaaaatatg aataagaaaa atttagctat attaatatca
     9421 ggtatgatgt ttttttctag ttcttcagca atttttgcag acaatactac aaatcaagaa
     9481 agattaatag gtaaaacaag atatgaaaca gcagtagaag taagtaaatt gggatgggtt
     9541 caatcaaaaa cagccattat agttaatggt aattctattc aaagtgcgct ttgtgcaaat
     9601 ccatttgcta agctaaagaa tgcacctata cttttagtaa acaacaacag tattgaaaat
     9661 tcaactaagg ctgagcttaa aaggcttggt gtagataatg tgtacattgt agatagtgga
     9721 aatagcattt cttctaaagt agaaaatgaa ataaaatcac ttaacataaa aataaataaa
     9781 atagtaggaa acaatatata tgaaatgtct acaaatgtac ttaaagaaat agataagata
     9841 aaaaaggtag aaaatgttgc agttgtaaat ggactaaagg gactatctga tgcagttagt
     9901 atagcttctc cagcagcaat acataatatg cctataatat tagtatcaga tgaaaacagc
     9961 aatgatagta gtataaattt cttaaacaat aggtctataa aaaaatctta catagttgga
    10021 ggaaataaga ttttatcaga caatacagta aagaaatatc caaacagaga aagaatcagt
    10081 ggttcagata gaaacaatac taatgcaaaa attattgata aattttataa agataaaaaa
    10141 ataaataatg tatttgtagc taaagatggt attaaccaag aaaatgcatt agtagattca
    10201 ttatcaatag gagtactagc tgctaaagaa aattctccta taatgatatc taatggtagc
    10261 cttgtaaata gtcaaaaaga atttattaaa aataaagata tagatgaaat aacacaagta
    10321 ggaggaggat ataatcaaaa agcatttgaa gatattttaa aactaaaaaa tgtgaacata
    10381 agaaataaaa ctaaaaaaat atctttaata caggctaaag aaatattaaa acaagctctt
    10441 ccaggaagca atattttagt ttgttattat gatgaagatg ataatgaata tgactgcaaa
    10501 gttataaagg ataatatcct atatgagata gaaattgatg cctactctgg taaaataata
    10561 gaaattgaaa aagatgatga cttaaatgat aataatttag gtacacaaaa tatatcattg
    10621 gataaagcta agtctatagc attagatgcc attcctaatg gtaatttaat ctattgtaaa
    10681 tatgatgctg aagataacga atacgaggct aaagtaataa aaaataagaa aacatatgaa
    10741 ttagagataa gtgcatttga tggaagaatt gtagatattg acatggacaa tgattctgat
    10801 gatgactttg atgataatga taagaataac tctaatacta atgacaacag ttctattatt
    10861 agtgctgaaa aagctaaatc aataatgcta gataaagtgc ctggtgggaa aatagtaaaa
    10921 tttaaatatg atgaagatga caaagaatat gaaggtgaaa taataaaagg aaaaactgaa
    10981 tatgagataa caattaatgc aattactggt aaaatattag aatttgaaat agatgattaa
    11041 taaaaaatat atattctatt aattattaca agcaataaag ccataaatat tgaaattttc
    11101 aatatttatg gctttatttt atttttttta atttaaatcc acattatgcc attatatata
    11161 tctatgcact cttttaaatt aaaatcttgt ccatcatccc aaacctaata aagttagatt
    11221 ttaaacaata tattgtgata taagtaattt gtgtagaatt attgcgtgat ttactcatga
    11281 agaaacaata aggttaatat ataatttgac tatattaaga cgaaaggaga catatacaat
    11341 gaatacaata ttaaaagtaa ataacataac taaaacttat aataaagggc aacagccgag
    11401 tttagataag gtatccttta atgtatcaga aggtgaattt ataggtataa tgggagcttc
    11461 tggctctggg aaaacaactc tacttaatgt attatctaca attgataaac caacaagtgg
    11521 agatattttc ataaataata taaatttgaa atcgcttaat gacacttgtg ctgctgattt
    11581 tcgtaaggat aatttaggct ttatctttca agagtatttt ttactagata gtcttacaat
    11641 tttcgagaat atttcagtac ctcttacact tcagaaactc aatccagata aaatagaaaa
    11701 catggtaaag agtctagctg aacgatttgg catagcttct caattaaata aatacccaaa
    11761 tgaattatct ggtgggcaaa gacagagagc ttcagcagtt agagcaatag taaaacatcc
    11821 tagtattctc tttgcagatg aaccaacagg agctttggat tctaattcag ctactgaact
    11881 actccactca ctacaggaag caaatgagga gttgaaaaca acaattttaa tggtaacaca
    11941 tgatgcctat gtagcaagtt ttgcaaatag aattttgatt tttaaagatg gtcatatcat
    12001 taaagaatta aataaacaac agaataatag acatacattt tatgagttga ttattcaaga
    12061 aattgcaaaa ttggatacta aaagatagga ggataattta atgaacattt tttatacggc
    12121 aataaagaat cttaaaaaaa acttttcatt ctattctcta tatctacttt cagtctcttt
    12181 tgttattact gtatttttcg catttacttc attttctgtt aataagataa tgctagaaaa
    12241 aatttcaact gatggtagag ttgaatctat gtgtaatgtt atttcgatat ttttgatggc
    12301 atttgtcatc ttctatatgg catactcaaa tcgattcttc ttgagacgtc gtacaaaaga
    12361 attaggtata tatgctttac ttggctatcg taagtttaca atattatcgc tacttacctt
    12421 tgaaaatgta ttaatttgta gtggagcttt tattattgga ttggtattag gagcattggc
    12481 acataagggt attgtctttg gaattacagc actacttaat ttatcaattg ataattcaca
    12541 gataccattt tttaatataa atgcaataaa taaaacaatt atctttatag cattagttgt
    12601 tttagtctta gctgtatcaa atggtaaatt tttttataag actccacttg taaaattgat
    12661 aagatatgaa aaaagtgcag aaaaaaaatt aaagttcaga aaaattccag cctttatggg
    12721 gtttattatg attttaagcg ggtattgttt atcactagat attcttcgtg gagctaaatc
    12781 agtatgggta agtattggat tttatcaaat gggcttgata actatgatta tcattgtaat
    12841 tggtacagtt ctctttatca attcctttct tccatatatt atgcaaaata gtaaaaaaaa
    12901 taaacgtact ttctacacag atatgaagat aatagctatt ccaaacttta tctatcgtat
    12961 acgttcaaac tctaaaactc taattatgct tacattactc tctgctacta ctttgacagt
    13021 ttcaagtgtt atggcactgt ccttatacta ccctattgca tctgtttcgc gtattgcacc
    13081 ttcagagata gaatttagag tagaaaaaaa taaacaaata gaagatgtaa aacaaattgt
    13141 cagtcaatat acgtctaata cagatgatat tatttttaca cagacaaata tttataaggt
    13201 aaaatcttca tcaaaagacc ttccatttga atatgaaatt ggtacatcta agaataattt
    13261 atccaatagt agttcttcta aagaagttat ttcaagagaa tcaggatttg aatgtatatc
    13321 tttttctgat tattatactt tattaaaggc acaaaatcga acaaagctac taaaacaact
    13381 taccccttta agggataatg aatgtatttt aatgaaatat caaccaaata atgatggata
    13441 tagtgaaaat ggaacgattt atccattact tgttaatggt aaagaaacac ctgtagctgt
    13501 aagggaaaca tcattaaaca atgctatttc ttttgccaat agcgttggaa cacttatcgt
    13561 taatgatagt atatatgagc aaatagcaca aaataaatta ccaatgacaa gcattttaag
    13621 tattaatggt aaatcaatta aaaataatga aaatttatat agtaatattt cagattattt
    13681 agatggaagc ccttatttac aaggatattc tcacaggata aatgatttgt tatcaattaa
    13741 tagttctaca tttctgttaa tcggatttct tgtggtatta ttctttattg ctgttggaag
    13801 tattttatac tttaacaata tatcagcaat ttcagattca acatctgatt ataagatttt
    13861 acagaaaatg ggttatgata atatacaaat taaaaaaatt attaaaaaac aagtattgcc
    13921 attttttaat attccgtttt tacttggtat attggactgt atatttgcta ccttggttta
    13981 taagactgct ttgatgcaga atttacttgg aaattcattt tcacaataca tacctgtgat
    14041 tattgcaatt ttcttaactt ctgtagtgta tttgatatat tatatactta ctattaaatc
    14101 ctgttgtaaa attgtattaa aaaattaata tttaccaaat cgtcaagctt agaggtgtga
    14161 cgatttggta tacttcattt ataagtattt gatataattt aaaattatag attcttaggt
    14221 ttgatttagt cataaaatta atataattaa ctcataagga ggtgtataag attgacaaat
    14281 tttaatttaa aaatactatt tttaggaaac tacattctat cacttctctt catgggaata
    14341 tctacaagca ctgaaactta tatgaatatc caaagcatta ttcccttaat aatttcaaca
    14401 ttattactca tagacatcac atatataacc tcttgtaatg tccgagaaaa taacattatt
    14461 tcattgtttt gtggattact agcactagac agttggtatt tactcttatc atttgtaacc
    14521 acaccaatag gcactattat ttttaaaata ttaagcacag ttatagtgta tgtctctatt
    14581 aaatttatat ttttattttt gtttcaaggt agtggataca atttccgtaa gataacagac
    14641 attttattgc ttatcacctg cattggtgca attgtaggag catttatttc taacagaacg
    14701 tatgcttgta tgtacggaat acagtttgtt gttagtataa taagcttttt ctatattatt
    14761 ttttatcatt tgaaaagggt tgtttttgtc ataaaaagcg aaaaaaaacc tatatcgatt
    14821 tcagtattta taacaattat aggattcctt acttattatt tttttacact aagaatatca
    14881 aatcatattg gtaattttgg aatctatcta actgtactgg tattttttat gagtattcat
    14941 ggtattgcac ttaaagaaaa tgatagtttt cctatttcaa cagtttttaa cataaaacaa
    15001 agggctatat ttctttttat aagtatttta gtaattagct tggtcgtact atttgccaat
    15061 gggaattttt ctataatatt aatagctata aatgttttat ttatgctcat tttcctctgt
    15121 aacattattt tggagtttaa ccttagagat gaaaaaaata aagtagttaa aaatagcaag
    15181 tatacttttg cattagaaaa attacaacaa gaagaacaac taaaaacaga gttttctaat
    15241 tttttacatg atgacatatt acagaacctg ctttcaataa aaaacatgat gagtaagtca
    15301 tatcgcccag atgttcaaga tattatatat gaaacactta acaatctaaa tacatgtata
    15361 aggaagcaaa tgcaagacta tcatcctgtt attttaaaaa atttaacagt aaaagaaaat
    15421 tatcagaatt taattgaaag tgtttcttca tcctttccac aaaaaaatat ttgtgtttca
    15481 tttaagtgtt ctgatacttt attcttagtt gaaccatatg atatattgat atatcgactg
    15541 attaaagaat tattgacaaa tgtgtataaa cattcaaatg gtagccatgc atggatagct
    15601 ttagcactag aaaaaaatat aataaaatta aatattagtg ataatggtgt ggcaaataca
    15661 tcttttaaaa cgcctttaga tataactaaa attacttttg atacaacaaa acacaaaggg
    15721 attatatcaa ttaaagaaca agtagaaaat ctaggaggaa cgataagtgt ttctgataat
    15781 atacctcatg gagtttgtgt acagattaca atccctatga aaggagatgg ttcttataaa
    15841 tattttgtta gttgatgacc atatgctttt tgctaaaagc ttgtcaattg cactagaaga
    15901 atacaaagaa atcgaacagt tttattccac acaggacatc cataatcttt cagaaatgat
    15961 tcaagataaa aaaatagaca ttgtcttaat ggatataaac cttagaggct taagtgaatg
    16021 tgatggctta aaagtagctt ataatttatt agagactaat tcaactatta aaataataat
    16081 cttgactgga tacgacctcc cagtttataa acatgaggct aaaaaaatag gtatctatgg
    16141 attcctaaat aagaatatta gccctgatag tttaattgat tcattattaa aagtctatca
    16201 agggggaact tgttttccat ctgaaaatac tgaagtaatt attgaagatt taactactat
    16261 ggagaaaaga attttacagc ttatctgtag tggtaaaaaa agaaaaataa ttgctgatga
    16321 attgtatata agtgaaagaa cattatcaaa tcatttacaa cacatatatg acaagctaga
    16381 tgtttcatca tcagttgagg ctattacaaa agctattaaa atgggttata tttctccagt
    16441 atattaaaaa aagtgagtgt ctcaaaataa atcttctaac tcataaagca ctctcctttg
    16501 tattaaatca aatatatttt catcatttaa acaattaaga agaggcttct ctctattttg
    16561 agacaactcc attttaatac actatttata cataaacata ttttaaacaa ctaaaaagca
    16621 actaatatct taataaatat ctcaatacat atcttcataa cacatatcac cataattaca
    16681 agcagataaa gaactctcct catttaaata cgactcaaat tcatacccaa acagaattgt
    16741 tggttctata tatatttcca tatctgtata tctccatctt cttacttttg cctctataac
    16801 cttgtaaaaa tcttctaaac tatacccttc ttttaatctt ttatctataa gtgccttagt
    16861 tatactcaaa tcagctttaa aattttttcc tgattcatta tttaattttc ttataataat
    16921 ttcatgtatg ctttctggat ttttgtaagt agaatcttca gtcaaatctt catccgaatt
    16981 ttcaaaggta tttttattta aattcttttt atttaaatta ttttttaata attctttttt
    17041 ctttgtctca ttttctgttt catacccagt atgaattatc aaatcataat ctttgtctat
    17101 actctcttga tttatacggc tgtatatgtt ggtattactg ttcttaaaca actcatctct
    17161 atgtttatat ttcattgttt tttggttatt gttaatagtt gcatcaatgt atttctctat
    17221 atttttgaaa atatcaccat tctttgttac aatttctgca ttactacttt cagaacaaac
    17281 ataactatat acagaacaac aatttccctt tactccccta cttattaatc tcaatatacc
    17341 taaatctata aattcattta caagtctact tattgtagat atagatacat caaggtcaga
    17401 tgcagctttc cttactgtca tcataaattg tcctcttggc agagttcctt ttaatctttg
    17461 tatattttct tttctcatta catagtgatt aaaccttatt ctatttacat ctcttttttc
    17521 attaaaatat aatactgctg aatttaattg ataaaaacta ttactcatat tcccactcct
    17581 ccaatttaaa tagataattt gttgtaaagt ttttagattt ttaaataact tattaaatga
    17641 ttatttttat taaaaagtat tctttgatat tttcatttta aagttttaag tataataagt
    17701 taatagtgta gatttaactt ttttatttaa acaataaact taatattatt tttaatataa
    17761 aaattttatt aaatttagta tgtaattttt taatcttatt ttattccatc tatagtctta
    17821 tgctaaataa taactttgat aatatttccc taacttatgt ttcaactggt ttatattgca
    17881 atttatttaa ttacagctta tttgcataat aattcgtcca tatcataagt tgtgtattta
    17941 tataaataca caattaaaaa taaatcacta tggtatacta attattacga agactatact
    18001 tatatgcaaa gataaatata atgcatctta tatcttttat taattataaa tatcttttat
    18061 caattataaa aaattaaacg ctatcttatt tatcataaat tacaaaaaca taaaatttta
    18121 ttactatatt gctataaatt aattgataca cattttacac aataaggagg gctatatatg
    18181 acaaatgcta aaagtaaaaa agaatatcta aatcgtattt gcaaagtgca agactatata
    18241 gaagaacatt tacatgaacc tctttctctt gatgaacttt ctaatatagc aggtttttca
    18301 aaatttcact ttcatagaat cttcaaagca atagaaaaag aaactttggc acaatatgta
    18361 aataggctaa aactagaaaa tgcaactgct tttcttattc atagaactga catgactgtt
    18421 actgatattg cacactactt tggctttact gattcagcag ttttctcgag agcatttaaa
    18481 aattactata aaataagccc tataaaatat agaaacaatt atagcaagaa ttgcaaagaa
    18541 ccatataaaa tttctcagta caatagaagc atatcaaaac ctaaatgtaa gaatactaga
    18601 gaggttaaag gtgaaattga aattctagaa cttgataata tgaatactat ttacactaga
    18661 tatactggtt catatggtaa tctaaccagt actttcccaa aacttttaga acggttattc
    18721 acatatgcaa gtgagcagaa tctacttgga cttgaaaaca cacaaatatt agccatttat
    18781 catgataatc cacaatttac acaggagcat caattaagga ctagtatttg catgaccatt
    18841 ccagataaca tagttgtcaa agagaatgac aatcttggca atatgataat accttctgga
    18901 aaatatcttg tgggacattt tgatatttca aaatctgaat atagtgatgc gtgggatttt
    18961 atgtataatg aatggctatc aaatggtaat tataagctac gtgcctcctt cccttttgaa
    19021 gtatatctta acaacccaca caatcaccct caaaatagac acttggtcga tatatattta
    19081 cctatagaac ctttttaata acctcaccta atctattctt gtattaagga ttaatatatc
    19141 cctattttac ggtttgataa aattgataaa atgaggtgaa ccaatgaaag aaataaaaat
    19201 taatggagca aagatacaca atcttaaaaa tatagatgtt tctatcccca aagataagct
    19261 tgttgttgca acaggtgtca gtggctcagg taagtctagc cttatgtttg acattgtatt
    19321 tgaggaaggg cgtaaacaat atctgcaatc tttaggtata cttgcaggta ttgatagtga
    19381 ggataagttt gataatattc aaggtttagc tcccactata gctgttcaac aaaatattat
    19441 tcgccagagc aatcctcgtt caacagtagg cacaagaacc aatattctca acatgcttac
    19501 actattgtat tcagtggaag gtcaaataat gtgtactatg tgtgatactc ctataagcga
    19561 taatctaatt tgcaataatt gtggatatga ggaagaacga ttaagaccaa gttatttctc
    19621 atacaactct tcagatggaa tgtgtatgaa gtgctctgga catggtgcat attttgagat
    19681 taatattgaa aaattagtcc ttgataagca tgacacccta aaacaagtct tagatagagc
    19741 aaagattaca ccaggatatt tgagagtatt ttctaaaaaa ttcaacgatt atttagacat
    19801 acaattctta caactacctg aagaaataag aaatgaagta ctctatggtc attatgaaaa
    19861 tggtaaaaaa agttcctctt tatcaaaggt atttcacaat cgttacaaaa ggggcgaaga
    19921 tttaaatgga gtttatacta tgactgcctg ttcagattgt aatggtttta aaattggtga
    19981 agaagcacgt agagtacttt taaacggaaa acatattgga gaacttggaa aaatgacgat
    20041 tttagaaatt gatgatttcc ttaaactctt aataaatcaa ggtaatttaa acacatttgg
    20101 tacaaatctt gtaaatgata ttttatccaa aactcaacat ttaatagaat cacgattagg
    20161 tcatctatcc ttataccgag aaatgtcaac tttgagtggt ggggaaatgc aaagactatt
    20221 tttaaattca catctcgatt cagagatgga ctcattaatt tatgtgcttg atgaaccaac
    20281 agctggattg catgaatcag aaaaaataga catcttaaaa tcaataaaga aacttaaaga
    20341 tttaggcaat acggtcatag tagttgagca tgataaaaac acaattaaaa tggcagaaca
    20401 tattattgat attggtccaa aagctggtgt tgaaggtggt caattagtat atcaaggaga
    20461 tttagaaggt ctacttcaat ccaacaaatc acttactggg cagtatctat ctggaaaata
    20521 tccaatgcct aatagaacaa gcaataaaaa tattacacat actgataaaa taccttgtat
    20581 aactattcaa aatgcaaata caaataatct taaaaatgta actgtatcat tacctttgag
    20641 tgctatggtt ggaatatctg gaaaatcagg tagtggcaag agttcactta tatcagacac
    20701 cctacttcct cttcttagaa gccaatttaa taaccatact atcaataatc aaataaattc
    20761 ttcagagagt gaaataggtg aggaagacga ttatgcagtt gttgaaacta ttgcagatag
    20821 actgattggt actaaacata tttctggata ttcagagata tcccaatctc ctattggcaa
    20881 aaatatgaac tcaactcctg cttcttatat tggtatatgg gataagatac gtacactatt
    20941 tgcaggtcag ccagaatcgt tagaacgagg atttacagct ggtcatttct catttaactc
    21001 caaaggtgct tgtcctaaat gtagtggtag tggatatgaa aaggtctggt tgggaaataa
    21061 tctaagtata gaccatatat gcagtgaatg tcagggaaaa agatttaatg atgaatcttt
    21121 atctattaaa tataaaaata aaaatataca tgatatttta aatatgagtg tttctgaatc
    21181 tgtatatttc ttcaaggata ttccaaatat tgtttcacac ttaaatgtat tagaacaaat
    21241 aggtatgggg tatataaaat taggccaacc tacaccaaat ttaagtggtg gtgaatccca
    21301 aagaatcaaa cttgcaaaag aaattggtaa aaaacgaaat ggtaatatcc tttatgtatt
    21361 ggatgagcca actactggtc taagtctata tgatacttct aagttgattc aattattaga
    21421 tgaattggtt gaaaatggaa attctgtaat tgttgttgaa catgatatag atgtattaaa
    21481 tgtttgtgac tggattgttg agcttggtcc agaaggtggt gacaaaggag gttatataat
    21541 tgcagaaggt tctccaaaag ctctaaaaga aaatccaaaa tctataacag ggaggtatct
    21601 ttaatgaata aaaacaattg cttagactta atttttgaaa aagtagaccc ttcaagtgtt
    21661 ggtatgagtt cagaaaagct ttcacaactt gaacctttaa taatctcaca atatagcaat
    21721 ataaatggta taattattgc aagaaaagga aaaattgtct ttgaaaagta ctataatgga
    21781 tataatgctg atgatacttt tcatgttgca tcagttacaa aaagtattat atcagcctta
    21841 ataggtattg caatagataa aggatatata aaagatgttg accaaaaggt tgttgatttt
    21901 tttcctgaat atacttgtag tccttctgaa atatgtaaaa aagcagtaac tatacgtcac
    21961 ctacttacaa tgactgcacc ataccctttt aaaaatatga ctgagtcttt agaaagactt
    22021 tgcaaacaaa aagattggat aaaatatacc ttgaatcaac ttggtcaaaa aggcgaaatt
    22081 ggtactttca aatattcttc agctggaacg cacctacttt cagctattat aacaaaaact
    22141 actggaaaat ctgctcgtga atttgctaat gagtacttat ttaaacctat tggtatgaaa
    22201 gttataccag attatgatat gaaagatttt ggttttgaag atttctttgg aaaaaatctt
    22261 aagggttggg caaaagatag acagggttat tctataggtg gctggggact tacattgagt
    22321 gctagagata tgttgcgatt tggttttctg tatttaaatg atggtatttg gaataataaa
    22381 cgtattatat ctaaatcgtg gattgaagaa tcagttaaaa tgaatagtaa tgaatacggt
    22441 tatctgtggt ggctatgtaa aaaagatggt gttttgtggt attcagcaat gggaactgga
    22501 ggaaatgcta tttgttgtat tccacaatat gacttagtaa tagccatacc atctaaaata
    22561 gtgtttaaac ctcgtgatag atgggagttt attgaaaaat atatacttac ttctataata
    22621 gattaattac tctttcataa attaattact ctttcataaa ctaattactc ttagatagcc
    22681 aaaccctcca ttataacacc agacttttgt ttggtgttat tatgactaat actatatatc
    22741 taaaaggagt tgaatactaa tggacactta cacgacatca gaggtagcag atttaattgg
    22801 tgtacatcct aatacagtac gtttatatga agaactaaac ttaataccta aacctaagcg
    22861 taataccaat ggttatcgta tctttaatga ttttcatata gaacaattta gatttgctcg
    22921 tacagccttg aaaattgaag tggtacaaag gggattaaga aaagatgccc ttgagattgt
    22981 aagaacatca gcgaataggg actttgataa ggctattagt cttgtaaatc aatacttgca
    23041 acaattagaa atagaaaaat ctaatgccga ggaagcactt actattgttg cacagatttt
    23101 aaatggaaat actccagaaa caaataaact ctttttaact agaaaagaaa ctgcagaaca
    23161 cttaaacgtt acaatagata ctttaagaaa ttgggaaatg aatggtttgt taaaagtaaa
    23221 aagaaaacaa aatggttatc gtgtatatac tgatgaagat ataaaacgac ttaaaattat
    23281 aagcgcactt cgttgtgcaa actattctct ttcagctatt ttacgaatgt taaatgcttt
    23341 atcttctaat cctaatacta atattaaaac tgtccttgac actccaaaag atgatgacga
    23401 aattatctca gtgtgtgaca aactgataac ttcattgaaa gaagctgaac ataatgcaaa
    23461 aaccatgaaa tctcatcttc aaattatgaa aaaacaattt tcctagccct ccactctaac
    23521 tccagacttt gatttgtgtg ttatccttaa gagcaagaaa aattcaacac acaaatcaaa
    23581 ggaggaatag gatggaaaat aaaaatacta attctacatg gaagaaaaaa gtcattcttt
    23641 ttttagcaag tcaatatatt tccttatttg gctcatcact tgtacaaatg gcaatgatat
    23701 ggtatgtcac acttcagaca tcatcaggtg tatgggtaac agttttaact gtatgctcct
    23761 ttatacctca aatgataatt tctttttttg ctggtgtatg ggcagactac tacaatcgaa
    23821 aggtactcat aattgtatca gattcaacaa ttgctatatc tacattagtt ctaattctat
    23881 ttatgatgtc atcaaatggc acaacaagtg aattaatagc catatcagta atatctgtta
    23941 tacgttcact aggaagtggt gtacaaacac ctgctgtaaa cgcaatgatt cctcaacttg
    24001 taccagaaga acatttaatg cgttttaatg gtataaatag tagtctacag tctatagtac
    24061 aatttatagt tcctattgta gctgctgcaa tacttggatt tggtaataaa ataaatcaag
    24121 ttcttttaat tgatgtattt actgcaatta ttgctattgt attattaata ttcatcaaaa
    24181 ttcctaaaca tgatatacca aaacaaactg aaaaagtctc attttttgca gatatggtat
    24241 cagggataaa gtactgtttt tcagataagt tcattagaaa agtgcttttt acttatggag
    24301 cattcatgct tctttgtgtt ccatcaggat ttcttatagg acttcttatt gaacgaacat
    24361 ttggcaaggg ttatatatac ctgtctatta ttgaaatgat tggattttta ggtatggttt
    24421 taggtggatt gattatagga gtttttggtg gttttaaaaa tagaaataag accttttttc
    24481 tagcaatatt atcctatgct attttttcaa ttattctagg tcttgtaaca caagcttggc
    24541 aattttttat atttatgttt tttacgaatt tttcaattcc aatcattcaa gcatcagcta
    24601 tgactatgtt gcaagagcat gtagcaccac aaatgctagg tagagttctt agcttaccta
    24661 ttattatata tactggtttt attcctcttg gtatgatgat tttcggacca atggcagatg
    24721 taatttctat taataattta ataatcatag cagggattat acttttattt tttgctttgg
    24781 ctatccccta ttcaaaacaa ttttacaagc aaggaatatc taagtcaaat gaatccgagt
    24841 aatatattta aatagtttaa ctcgatttat ctggttaaaa atgaaggggc ttaaaatgga
    24901 ctttttaatt tatgaagaaa attttttata tgaaatcaaa tatattttat catttcaaca
    24961 ttgaaaaata ggtttatctt tattttgagt ccctctcttt atagcaaata atattttgat
    25021 actatcttta ttaactttat ataataatac ctttacttgt aggttataat gatgaaattt
    25081 atcaaatcat ataataatac ctccatttat atcataaatt aaatttatag tacaaatgga
    25141 ggaaaataaa gtatttatat agtttaagta cctattaagt tttccactaa gtagctctaa
    25201 ttgtactgaa cttcttacta aattatattg aataattata aaataaaact aactattaca
    25261 ctacataaat taaagtatac aaattgcact acataaatta cactatacaa agtatattaa
    25321 atcacctatt aaattgtccc aattataatt atactgagtt atctactaaa agataacttt
    25381 ctttttttat ctaaaataat atatatcata aaccatacta cacaatgtac agccaaaatt
    25441 acaatatctt tgataataat gacattacca ttaataatct ccataatacc attaaatgta
    25501 gcaactgaag gcattatata gcataaattc cctataccac tggtattaac ccctagaaga
    25561 tatattaaca aaggagtacc tataaataaa atcattacta ttttaatata aacaattcct
    25621 accataagac cttctgacaa ctttccaata aataaaccta taagtgatga aataaatgct
    25681 gaaaatacaa cgagtaacaa catcattatc atatctagta ttgaaatatc caaacatata
    25741 aatacagtta tcattgaaga taaacaccca cataaaagac ccaaaaatac tttttgtata
    25801 atatactgac ttctactctg tggcaaaact tcatttataa atgatattcc atcttctttt
    25861 tccgatataa tattcattgc attaaaggtg catcccataa acatagcagt aattaaaatc
    25921 atagcaataa aaacattttg aaaccctttt aaaatactat atttaggcaa tattgtaacc
    25981 tcaacatctt ttaaatcacc ctttagtcta aacatctctg gcaatgtctt tccaacattt
    26041 tttatagcat taatctcatt tccataaagc acagttttta tccctttacc atctctaaca
    26101 actccaattg tgtccgtttt aatatcctta atttcacgta ttaaatgcat ttttgtgtca
    26161 tatacactta ccataccata cttcttcaac cactgctctg tttgtacagg aagtgtatcc
    26221 ttcactatac caaaacgtat ctctcctaat gaagatatat caattgttcc cataaaattt
    26281 attaaaatcg ccataacaac tggtaaaaag aaggaaatta tacacatttt atcttttttt
    26341 atactcttta actggtaacg taatcctatc aagattatcc ctccctaacc aactctttat
    26401 taaaaatata ttttacaagt aaaaatagaa ctattattgt caaagaacac actacaaaat
    26461 aaactaattc ttttgaaaaa ttcatgaaaa acgcattttt aagctgacta taaacatgat
    26521 agaagggatg atatttaatc caatctattt caaaagatgt atttgcagat agaaatatag
    26581 gtgtagttat gaatacagca accacaagat atataagaga aaattgctta aaatctctca
    26641 ataacattga gcatcctatt ccaatcaaag acataataat tgataaaata ctagcttgaa
    26701 ctaatacttg tggaagtatt tttattcctt ctaaaaatcc aatattaaat actactaaaa
    26761 gaactgtaaa acataggtca catataaaaa aagcaaatat ctttgataat ataaaaagac
    26821 ttctttttac aggtaaaata ccagtaatac gcattactcc catctgcttt tctttaaata
    26881 ataaagaggc aatccctaaa aatccaatag ctacaatttc aaagaataaa atctcacaag
    26941 tcatgtcttt tctttgtttt atctcagatg tatccttatc attaatttga attattgcat
    27001 tgttgtctga tattttgcta ttatggtctt tttctgatga taataatgac aatgcatagt
    27061 cagctctata attgtcaact cctttaatac tttcatgtaa aattactttc ggcttactgg
    27121 aactcatatc aatacaaact gcacttgtat cttcatcaag ttttgctttt aattcaccta
    27181 tagaagaaac ttgattaatt aatggagatg cactcatgct tatattctct ggattatata
    27241 gatatacatt attgtgataa ctatcttcat ccataaactt tacataacca aaattaatat
    27301 aacacgaata aagtatgaga gagccaagtg tcagcacaaa aaatttacca taaatcatca
    27361 tccaaaaatc tttttttact aaggtaaaaa atttagaata cattacacta acatccttcc
    27421 tgtgtattta ataaaaactt cttctagtgt aggttctttt gaatgtacac ttaagatttc
    27481 atcatattta aaagttattc cagattctaa ctcttgtata tctacaattt caatatctcg
    27541 atttccatta tatacaaatt ccacttcaac tttattattg ctatttttca tttttaaatt
    27601 tttgggttta tctattacca taatctttcc atctgcaata aaagccacat tgttacacat
    27661 taaatctgca tctagcatat tatgagtagt caaaaagata gttgttcctt tttttcgttc
    27721 ttcttcaata atcttacgaa atagtacagc tccagatggg tcaagtccac tagtaggctc
    27781 atctaaaaat aacaactttg gattacttac caatgctctt gccatgctaa tacgttgctt
    27841 catacctttt gagtaggatg atacaggctt atttataaac tctttcttaa actctaacaa
    27901 atctaagagt tcatatatgt ctctcctttg atttttagga taaaaagacg aaaaatattc
    27961 taaattatca attgcactta aattggtata taaataagga aattcaaata aaacaccaat
    28021 cttttgatat agagactctg tatatgcttt aacttccttc ccaaaaatgg atacgtgccc
    28081 attgtagcct tttaaaacgc catttaagat tttctgtgtt gtggattttc ctgaaccatt
    28141 tgaaccattg acgtatagac aagtttgctg aatagcaaat aaagattaac taaaatgata
    28201 taattaacta attattgtat tttagttttt tagaaaagtg gttgtccttt ataaattcct
    28261 tcaacctctt atattgatct gcaaatttaa attgaatagt aatttcatta ttttcatgaa
    28321 tataaatagc atctatcaat tctattaata ttcctctatt aagttctttt atatttttat
    28381 acttcaaaaa tgtttttaga tatggatcat tatctccaac tccttttgaa gatgattgta
    28441 tttcagcttc tatattagat ataatacgtc taatttcctc agctttaatt tcaaacctcg
    28501 atttcattct agcatactca tttctactaa tttcttcact tttccaatcc atatatagat
    28561 tatctgtaac acaaataatt ttttctaatt ctttttgttt ttcattaagt tgtttcttta
    28621 atcttgatga ctgattgaca attatggctt gcttattaat ttcagaaata acttcgctca
    28681 tattttcaac catagctatc tgtatctgaa ttgcctttaa aactgcttct tccaatatct
    28741 ctagtttaat ggaatgtcta ctacatttag atttattttt caatgcatat gtagaacata
    28801 tataataatt atattcttta acagttccgt catttaactt atttttattt gttttctttg
    28861 tcatacttct tttacagtca gcacatctta aaaatccaga aaacaagtat acatttcttc
    28921 cttttggaga cattcttata tctctttgat ctaattcttg agcctttaag taagtatctt
    28981 tgtcgataat agcttcatgt gtattttcta caatgaacca ttcattttct ggagtgcaaa
    29041 tagttttatg aactttataa cttataactc tttgtcgtcc ttgtaccata tctcctatat
    29101 acattcgatt ttttaatata gtagatattg tattcataga ccacatacca tcctgtttat
    29161 ctagctttgg attttttaac atcaaacctt tactatgttt atatttggta ggtgttaatg
    29221 ttccaagttc atttagacgt cttacaattc ctaatttatt tatcccctct tctactctcc
    29281 aacgaaaaat atctcttata acttgagcag cttcctcgtc aataataagt ttattcttat
    29341 tatctggtga ctttttatac ccatatggag caaaagcccc tataaattct ccattacgac
    29401 gttttgtatt aaatgttcgt cttatatcag cagaagctct tgcagcatac ctgtcattca
    29461 ttaatcctgt aataggaaca tctaatccat cagtaattac ttctgggttt aaataagtat
    29521 caaatttagg acttcccaag caaatgaatc ttgtcctatg caatggtaaa aattcttcta
    29581 gaaaatatcc ttgatctgaa taatttctaa atgccctggc taatgtctta caaataatac
    29641 aatttacttt tccttgcttt acatctttaa tcatacgctg aaaactttca cgctcatcat
    29701 cagttcctgt taatccatca tcaacataaa aatcaactaa aacatattca tcttcaaaca
    29761 tttcctctaa atattcttta agtatctttt tctggtttat aacgcttaaa ctttcatcat
    29821 ttccatcttc acgagaaagt ctaatataaa cagcaacttt ccatatagtc ctgttatttg
    29881 tcaatcttga ctcctgtgat tttcttctac gtggcatatt tcctccaatc ttctataatc
    29941 acaacaaaat aataacatat ttcacaacag ttttcagcag tttttgaggt ttttaaaata
    30001 gcttatcaaa cataaatcta gtgtattttc actatctgaa aattttaacc ttacaacaac
    30061 atctccgcat ataaaacaat atggactttt catctctttt aaacatcttt ctaaatcttc
    30121 atcctctaaa tctttttgtt tattgataac tttatcaata tctatatact ttttatttaa
    30181 tttttcatta tcttttttta ctatatttac actcataaat tctcccccaa taatattact
    30241 tcaattagta agataacttt atttaaagcc atcaattagt taactctgca tgtttctcat
    30301 ttatatccat catctaacta atagcattaa tgatatacca aaatataata tattaggtca
    30361 tgtaaaagat ttaataattt ttttaactta ttgttataga taactaatag aatactctat
    30421 atttaactaa acaattatac ggctctggca agctccacag gaatttcacc ttatcccatt
    30481 cttatgggta taccgttgtt tcaaacggga gtatcattat actaatatat aaatcatggc
    30541 agccattttg ccaaaatgac ttacagatca ctaactttaa tcgctctctt ttttaataaa
    30601 aggtcttggc gaattagtct gtcggctcat tatatattaa gtgtatataa ttgttttatt
    30661 cacttttcaa agatccctct agacctattc attgaatatc ggcctattaa aatatattag
    30721 aatttaatat actttaataa actgatattt ttctcagcta gagaagtaca aaataggatt
    30781 aaaatactcc tctagccaaa tttatcttat acgaatttaa attttaaaat tttataaata
    30841 agctttaatt caagtaaatt ctttttttct aaatctaaac atttataagc atttcctcgc
    30901 ttatcatata gagtctttgt gcaaagttta acaatgtagg cttcataatt ttttaaaact
    30961 attcctatag caattatgtc accctttgca gctgcataaa taatatcgta tggtaataat
    31021 ttattattct tatgtaccaa actcattttt ctttaccccc aatatttttc ttaatgtttt
    31081 gattgcaaca tttctttgat accacacagt atcttgacta acatttaaaa tgcctgaaat
    31141 ttccttatcg ttgtacccaa taaaatatga aagcaaaatt attttttgtc ttaattctgg
    31201 taataattta atagcttctc ctaaagcttc atttactata tgaattttaa atccatttac
    31261 attaaagcta taataatcac tcaaatatag atcatttgaa aaaaggctat tcttttgttc
    31321 ttctgataat tcgcaaaata aaatttgtac ctgcctaaga tatctattct ttctctcaat
    31381 atcaatagat tcatttttta atattttttt acaaaagcta tcaaacttat gtatcgtgga
    31441 ttctttattt attgaactat ttttcatttt ctcgcctcct ctcgcattaa gctaaagtgg
    31501 tgataaatat cctttttctt ccccccttct actattacca cgaatgagag actcgtaatt
    31561 ccgtaaaata tatacaaaaa gcacataatt agtcatagaa ctaactacat gcttaaaaac
    31621 tttcgtatat cttttataaa ccaaaaatct tatttatttc ctttgtatta aatctagcat
    31681 aaccttttaa tgcaacaaat gcgataaaaa tacttctcaa atatattcat agttataaat
    31741 ttaaaaatta aaaaattcct aaaaataaaa agatgagtac tataatactc atctttttat
    31801 tttttaattt tgtgtgagtt ttagttggta atagtcatta accttaaaga aattttagtt
    31861 tgtatgcgtt atgactataa tatttagggt atgttttatt tatatcacat ttagccaaat
    31921 tatatctgtc tatatttttg tataatatga atatatttga aacttttata ttttaaacct
    31981 atctttaact agaaattata tattattaaa tattttcaag atttttatca ttttatataa
    32041 tatattcatt taactatatc gtataaagat agaaaaaata tataataaaa atgcgagtag
    32101 caatattact actcgcattt ttgttatatt gtcttaaaat atatagattt tgtgaccatg
    32161 aattttattt aataagacaa tttaacaaat
//
LOCUS       DAGSWI010000035        32034 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.035, whole genome shotgun sequence.
ACCESSION   DAGSWI010000035 DAGSWI010000000
VERSION     DAGSWI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.035"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            396..1562
                     /locus_tag="MJF80_001471"
     CDS             396..1562
                     /locus_tag="MJF80_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBZ0280141.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            1584..3230
                     /locus_tag="MJF80_001472"
     CDS             1584..3230
                     /locus_tag="MJF80_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBZ0280142.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMRWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            3327..4700
                     /locus_tag="MJF80_001473"
     CDS             3327..4700
                     /locus_tag="MJF80_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0280143.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWAKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGSINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQDYIG
                     VIFSSAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            5145..6098
                     /locus_tag="MJF80_001474"
     CDS             5145..6098
                     /locus_tag="MJF80_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBZ0280144.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            6136..7098
                     /locus_tag="MJF80_001475"
     CDS             6136..7098
                     /locus_tag="MJF80_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBZ0280145.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            7123..8109
                     /locus_tag="MJF80_001476"
     CDS             7123..8109
                     /locus_tag="MJF80_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBZ0280146.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            8122..9168
                     /locus_tag="MJF80_001477"
     CDS             8122..9168
                     /locus_tag="MJF80_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBZ0280147.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            9335..11065
                     /gene="lpdA"
                     /locus_tag="MJF80_001478"
     CDS             9335..11065
                     /gene="lpdA"
                     /locus_tag="MJF80_001478"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBZ0280148.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            11483..14431
                     /locus_tag="MJF80_001479"
     CDS             11483..14431
                     /locus_tag="MJF80_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0280149.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            14409..14873
                     /locus_tag="MJF80_001480"
     CDS             14409..14873
                     /locus_tag="MJF80_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0280150.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            14953..15255
                     /locus_tag="MJF80_001481"
     CDS             14953..15255
                     /locus_tag="MJF80_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0280151.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            15317..16681
                     /locus_tag="MJF80_001482"
     CDS             15317..16681
                     /locus_tag="MJF80_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBZ0280152.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            16716..17189
                     /locus_tag="MJF80_001483"
     CDS             16716..17189
                     /locus_tag="MJF80_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280153.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YVKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            17361..18011
                     /locus_tag="MJF80_001484"
     CDS             17361..18011
                     /locus_tag="MJF80_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBZ0280154.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREGINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            18185..19117
                     /locus_tag="MJF80_001485"
     CDS             18185..19117
                     /locus_tag="MJF80_001485"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBZ0280155.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYYDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKNRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            19391..20104
                     /gene="ispD"
                     /locus_tag="MJF80_001486"
     CDS             19391..20104
                     /gene="ispD"
                     /locus_tag="MJF80_001486"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBZ0280156.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            20101..20586
                     /locus_tag="MJF80_001487"
     CDS             20101..20586
                     /locus_tag="MJF80_001487"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBZ0280157.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    20681..20889
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20953..22668
                     /locus_tag="MJF80_001488"
     CDS             20953..22668
                     /locus_tag="MJF80_001488"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBZ0280158.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            22790..24235
                     /locus_tag="MJF80_001489"
     CDS             22790..24235
                     /locus_tag="MJF80_001489"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBZ0280159.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            24673..26154
                     /locus_tag="MJF80_001490"
     CDS             24673..26154
                     /locus_tag="MJF80_001490"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBZ0280160.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            26375..26530
                     /locus_tag="MJF80_001491"
     CDS             26375..26530
                     /locus_tag="MJF80_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280161.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            26675..28072
                     /locus_tag="MJF80_001492"
     CDS             26675..28072
                     /locus_tag="MJF80_001492"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBZ0280162.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLGMG
                     IVLEDTRQGVKWKRI"
     gene            28057..28470
                     /locus_tag="MJF80_001493"
     CDS             28057..28470
                     /locus_tag="MJF80_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBZ0280163.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            28576..29334
                     /locus_tag="MJF80_001494"
     CDS             28576..29334
                     /locus_tag="MJF80_001494"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBZ0280164.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLKEVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            29347..30084
                     /gene="rlmB"
                     /locus_tag="MJF80_001495"
     CDS             29347..30084
                     /gene="rlmB"
                     /locus_tag="MJF80_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBZ0280165.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            30087..30614
                     /locus_tag="MJF80_001496"
     CDS             30087..30614
                     /locus_tag="MJF80_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBZ0280166.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            30679..31326
                     /gene="sigH"
                     /locus_tag="MJF80_001497"
     CDS             30679..31326
                     /gene="sigH"
                     /locus_tag="MJF80_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBZ0280167.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            31400..>32034
                     /locus_tag="MJF80_001498"
     CDS             31400..>32034
                     /locus_tag="MJF80_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBZ0280168.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTDKP"
BASE COUNT        13035 a         3372 c         6120 g         9507 t
ORIGIN      
        1 ttggataggt tctatcctat ctctacatat aggtttccct atatgctgac tattttaact
       61 actaactcat gactaaagtc acgagtgtgc gtagctattt tttaatcaaa tagtttgaaa
      121 ttaacatcta aattatcaat aggttcaatt atttcatgtg aaaaattatt tctgttcata
      181 ataatatcct ccttataatt ttaaattata aatagtaaat ttataatgtt caatattaaa
      241 aagataatat agactaataa attagataat aaatatactt aagaaaacgg ataaaatcaa
      301 atataataac tgtatattaa aataacttaa aaaaattatt ttattataag tgcaaaatat
      361 acctatttaa ataagataag aggggaggat tttatgtgta tataaaaggt gtaaatttag
      421 gaggttggtt agttttagaa aaatggatga cctcaagttt atttgaaggc acagaagctg
      481 aagatgaata ctatttacca agacagcttt caagagaggc atatgaatct agaattaaga
      541 ctcatcgtag tgaatacatc acagagagag attttgcaac gattaaatct atgggattta
      601 attcagttcg tataccagta ccatacttta tttttggaga ttgtgaacca ttcataggat
      661 gtgttaaaga acttgataaa gcatttgctt gggcagataa gtatgggctt agtatattga
      721 tagacttaca tacagttcca ggaagccaaa atggatttga taatggagga atatcaggta
      781 tatgtagctg gtcacaaaat ccagaatatg tagcgtttac attaaatgta ttagaaagac
      841 ttgcaaaacg ttatgggatg aggcatgaat tgtatggaat ccaaatttta aatgagccta
      901 taacagaaag aatgtggaac ataatgaatg ttccaaatcg gtttaaggct gtagataagg
      961 agatggcaag ggggagtaaa cctaattcat tagagttttt acgtgatttt tatataaaag
     1021 catataaggt aatgcgtcct tatatgagag aggaaaacgt tatagtgttt catgatgcat
     1081 ttgaattaaa agcatggaaa gactttatga gagaggaaga atttaaaaat gttgttctag
     1141 atacacacca atatttgatg ttagctgaag cagatggatg tgaacaaagt attgatagct
     1201 atctcaaata tattagagaa aattatgcta aagatatttt acaaatgcaa aaatattttc
     1261 ctgttatatg tggggagtgg agcttattta attcatatgc ttgtggtata gatacaaatg
     1321 gaggtcaatc acctctaaat gggattgaat caaatataga taaattatca aaagacgata
     1381 aaagagaatt atataggaag atagcaaaag cacagcttga cgcatggaga aatggaagtg
     1441 gtcactacta ttggaattat aagttattac tagatacagt taatgaagaa ggttggatag
     1501 gatgggatag ttgggaccta ggcaaatgtg tagctcaaga atggtatcct atagaatatt
     1561 aaactaaaat tggaggaaaa gatatgatta aaaaccctat acttccaggt tttaatccag
     1621 atccttgtat ttgtagaaag ggagatgatt attatcttgt agtttcatct tttgaatggt
     1681 ttccaggtat acctgtttat cactctaaag acttaaaaaa ttgggagtta tatactcata
     1741 ttttgacaga tgaaacaaaa atagatttga agaagcttcc ttcatctaaa ggaatttggg
     1801 caccttgttt gacttattgt gaagaagaag atttatttta catagtttat ggaattatga
     1861 actctatgaa tgcaagatat tttgatgttg ataattattt aattacatca aaagatataa
     1921 agggagagtg gagtgaacca gtatatctac actcatcagg ttttgatgcg tctatttttc
     1981 atgatgatga tggtaaaaag tggattgcat cattagactg ggagactaga gaaggatatg
     2041 aaaaacctgg tgttatatgt ttagtggaat actgtacaaa aaagaaagaa atagtaggat
     2101 atccaaagag aatatggtca gggggaactg atagaggttg tattgaagca ccacatataa
     2161 caaagcgtgg agattactat tatataatgt gtgcagaagg tggaactgga tatggtcata
     2221 gtgtaactat gggaagggct aaaaatatat ggggacctta tgaaaaagat tcaatgaatc
     2281 caattgtaac ttcaattcca ggcgattttt atgaaagaca tgaccctgac catctaaagc
     2341 ctaaatacta taacccagaa tcaaaacttc aaaaatctgg tcatggaagc tatatagaaa
     2401 caacatcagg tgaagtatat ttagttcatc taacatctag accatttgta ccagagttaa
     2461 ggtgtacttt aggtagagaa acagctattc aaaaaatgag atggacaaag gataattggc
     2521 ttcgaatgga agatgaaagt aatttggcaa aagaatacgt atcagaaagt aaactagaag
     2581 aacatttagt ttcatctata ccaagttttg atgactttga ttcaaatgaa ttaggtttac
     2641 agtactatgc tcctaggatt tcaccattat catttgcaga tgttaagtct agaccaggat
     2701 atgtaaggat tagaggacaa gaatcaagaa catcattaaa taaagtaagc atattggcaa
     2761 gaaaattaac ttcagtatat gcaagaataa ccactaagat ggagttttac ccagaggtac
     2821 atcagcatag tgcgggtttg ataatgtatt atgataatat gaattatata aatttaagaa
     2881 agtattatag tgaaacattg ggtcaaagtg cactatcaat aatacatctt gaaaatggag
     2941 agaagacgga atttttaaat actaggatac caataaaaga tataccaata tatcttcgtc
     3001 tgtatataca aggtcgtaag tcatattttg aatggagtta tgatgaaaaa aactaccaga
     3061 gaataggaaa ggtatttgat acaactaagt tttcagatga gtactgtaaa tatggagaat
     3121 ttacaggtac gtttataggg ttaacttgtg cagatcgtgt aaagcataaa cactatgctg
     3181 attttgattt ttttgaatat atagtagatg aatcaaaaga tgtcgattaa taaaatcatt
     3241 actaaaattt tcagagcaaa ggtaaatctt atgctctgaa ataagaaaca ttagaaggca
     3301 aatcttacaa aaaggagtat ggtattatga atacagaagt taatgttaaa gcaactacta
     3361 ttagatatga aaagatttca ttttttgaaa gaatttctta tggttgtggg gatttggggt
     3421 gcaatatcat atactcagct atgtcagcat ttttgctgtt ttattatact aattatgcag
     3481 atgttagtgc agcagctgta ggtagtatta tgcttgtatc taggatactg gatgggttta
     3541 gtgacttaac aatgggaatt atagtggata gaactaaatc caaatacggt aaggctaggc
     3601 cttggatttt gagaatggcg attccttttg ctatagcagc agtattatta ttttctgttc
     3661 catcaaatct tggtatcact tctaaattaa tttatatttt tattacatat aacttagttt
     3721 ctactgttat ttatacagca atcaatgtac cctatgcaac tctaaattca cttataactc
     3781 aaaaccagta tgaaagagga gtattaagta tatttagaat gatattagcc acttgtggta
     3841 ccttactaat aaatggactt acactacctc tagttgaata ctttggaaat aatttatcag
     3901 catgggctaa aacattcttt gtatttggaa tagcatctat aatggtattt tttataacat
     3961 ttactggaac caaggaaaga gtaaaagcag ttaagcaaga taggaatgaa gtaataccat
     4021 ttaaaatagg tattaaatct ttatttagaa ataagtactg gatacaaata acattatgtt
     4081 tagtttgcat atttattgta tttgcgataa atggtggttc atccgtatat tatgcaaagt
     4141 ttattttggg agatgaaaaa ttatttggtt ctataaatat ggtttcaaat atatcccaaa
     4201 ttatagctat gtttatggtt gctcctttta taaaaaaatt tggaaaaaga aatgtattaa
     4261 tagtaggttc aataatatta atatcgtcaa atgttatgtt tataatagtt ggacaagact
     4321 atataggagt aatattttca agtgctataa agggaatagg aagtgctgga atagcatcaa
     4381 caatgtttgc tattgtttca gatactattg aatatggaga atggaagact ggatatagaa
     4441 ctgaggggct tataaatagt gcatctagtt ttgggtttaa agtgggaaat ggattaggtt
     4501 cagcaatatt aggtgcagta ttatcaattg gtggttatgt aggaacatca gctactcaaa
     4561 gtgatttagc tatagtatca ataaaagtat gttttatata cctacctata tttataacaa
     4621 tattacaaat tatcataatg tctttttata agcttgataa agaatatagt actatactta
     4681 atgaattaaa tactaaataa tgaaaaagtt tcttataaag tcttaagttt aattaaggtc
     4741 tattgtaaat gttccctata aaaactatta tatatttatg attattgaag aatttttaga
     4801 gaagcgaata ataaaaatat aagaagactt taaatgatga aatggttggt tttttagaaa
     4861 tgatttaata agttattgtt atttaaagca ttataagtgg acaattaatt tttaatttat
     4921 ggagatgata tttaacaatt agaagtaata tataatatgc actaagaagc aatttttcaa
     4981 ctatttatga aggtggtggt acaagaaaag tatcaccttt tttttgttat acaaaaaaac
     5041 agttgacttt ttatatgaac cccttctata atgttactga aattaagtaa aatgattgaa
     5101 caaatgtaaa gataattgaa aagcttgatt ttggaggaaa gcaaatgaaa aaaattggag
     5161 acttaagatt aatgatgaag tgttgttcac tttattacga agataatttg aatcaacagg
     5221 aaatagcaaa tcaattggga atatcaagac caacaatatc aagaatatta aaagaagctt
     5281 ttgaacaagg aatagtaaaa atacagatag ttgatgtttt gaaaaatgat taccaaaaaa
     5341 tagagagaag tttggagaga aaatataaat taaaagaagt aatagtagta gatgataaac
     5401 aggatgcact aacacaaaaa caagaactag caagagctgt atctgaatat ttaactagag
     5461 tagtaaagga aaatgatata ataggtgttt caataggaac aacactaaaa gaaataccaa
     5521 gatatgtgga aaaaagtaac tgtaaaaatg ttacatttat acctctatta ggagggattg
     5581 gagacaatga aatagatatt catgcaaatc aaatagccgt aagtttagcg agggcttttg
     5641 gaggtgattt taaattatta catgctccag ctgttatgtc tgacttgtct acaaaagaga
     5701 aactttgcaa agacgaaaaa attaaagaag tgctagattt aatagataaa acaactatag
     5761 caattgtggg tataggaaat cctatgagtt taaactcaac aataatggct tctggataca
     5821 tgaatgaagc agatatagaa gatttaaaaa aatacaactc aataggtgca atttgtttac
     5881 aagcctttga taaagaagga aaaacttcta tacttgaatt taatcaaaga gtattaggag
     5941 tgaaactgga agatttaaaa aagataaaaa gaacaatagg tgtcgcttca ggagatgaaa
     6001 aaatagaggc aataaaggca tcattaaaag cgaaatttat aaatagttta gcaattaatc
     6061 acagtttagc acttaaatta ttagaagatt gtgactaata gaaagataga taaaagttaa
     6121 caggagggaa tgtatatgtc aatatcaaaa gaaacattgc ttgaaatgta taagagaatg
     6181 aatcaggcta gaaaatttga agagaaagtt agttggtttt ttgcaagagg tatggttcat
     6241 ggaacaactc acttatctgt aggtcaagaa gcgtcaagtg tggcggctgt aatggcttta
     6301 gaaaaaggag atttagtatc attaactcat agaggacata gtcaatttat aggaatggga
     6361 atagacctaa ataaaatgat ggctgaactt atgggtaaag aaactggatt ttgtaaggga
     6421 aaaggaggct caatgcatat agcagatatt gagtcaggta acttaggagc aaatggagtt
     6481 gtaggtggag gtcttacaat agctccagga gctgcactta cacaacagta taaaaaaaca
     6541 ggcaaaatag ttttatgtag ttttggtgat ggagcatcaa atgaaggtac ttttcatgaa
     6601 ggaataaatt tatcttcaat atggaaattg ccaataatat tttactgtga gaataattta
     6661 tacggtatgt caacaagcat aaaacgtcat atgaatatag aaagtattgc cactagagca
     6721 gcttcttatg gaatagaagg aatatcaata gatggatata acccaataga agtatacgaa
     6781 acagtacaaa aagctgctga gaagtgtaga agaggagaag gacctgtatt aattgaaagt
     6841 agaacttata gatggttagg tcattcaaaa tcagatgcaa atgtatatag aacaaaagaa
     6901 gaaatagaat catggaaagc aaaagaccct attgaatttt taaaaaatta tcttatagaa
     6961 aataatctat ctaatgaaga tgaattagat aagattcaag aatttgcaaa gcaatcaata
     7021 gaagatgcag tagagtttgc tcaaaatagc ccaaatccaa aaatagagtc tttattagag
     7081 gatgtatatg cagactaaga atggtaaagg aggaaaataa aaatgagtac aagggaatta
     7141 acatatgcac aagcaataaa ggaagcaatg tcagaagaaa tgagaagaga tgaaaatgta
     7201 atatttatgg gagaagatat aggaatatat ggaggagcat ttggtgtatc agttggtatg
     7261 atagatgaat ttggaccaga aagagtaagg gatactccta tatcagaagc agctatagca
     7321 ggagcggcag caggtgcagc agcaactggc ttaagaccta ttatggaagt aatgtttatg
     7381 gactttgtaa caatttctat ggatgcaatt gtaaatcaag cggcaaaaat gagatatatg
     7441 tttggaggaa aagcacaagt tccaatggtt gttagatgcc caggaggttc tggaactggt
     7501 tcagcagagc aacattcaca aagtttagaa gcttggtttt gtcatgtacc aggagtaaaa
     7561 gtagttgcac cttctacacc agcagatgct aagggattgt tgaaagcagc tataagagat
     7621 aataatccag tcatatttgt tgagaataaa ttgttataca gaaaaaaagg ttttgtgcca
     7681 gaagatgatt atgtaattga aattggaaag gcagatatta aaagagaggg tactgatgtt
     7741 acagtaatta cttatggaag aatgttacaa agtgttgaag aagcagcaga gactctatca
     7801 aaagaaaata taaatgttga aattatagat ttaagaactt tatatccatt agataaagag
     7861 actatagtaa agagtgtatg taaaactggg agagttttaa tttgccatga agcagctaag
     7921 actggtgggc taggtggaga aatatcagcc ctgataacag aaagtgaatc atttgattat
     7981 ttagatgctc cagtaaaaag aatttgtgga aaagatgtgc caataccata taatccagag
     8041 ttagaaaaag cagttgtccc aagagttgat gaaatagaag aagcaataaa atctttaatt
     8101 gttagataag gagggataga tatggtggca aataaaataa aagccacacc agcagctaga
     8161 agtcaggcaa gaaaagacaa tataaaatta gataggctga ttggaagtgg agaaaatgga
     8221 aggattcatt tagtagatgt gttaaattat ttaaaagata ataaagcaaa tactactcct
     8281 ttagctagaa gaatagcaga agatttaaat atagatttag aaactattgt aggtactgga
     8341 tataatggaa agataagaaa gtgtgatgtg gaaaaattaa ctggaaaaga aactattgta
     8401 tcaactaata catctaaatc tagtgagaaa aaagagctga aaattgaaaa tgaaaattca
     8461 agaatgttta acacagtaga aggaatattt gaaaagccaa atccaatgag agctactgta
     8521 gcaaaacgaa tgtcagaaag ttattttagt gctcctgtat ttacattcaa tatagaagta
     8581 gatgctacag aattgaaggt acttagagca aagttaatcg acacagtaaa agaatcaact
     8641 ggagtaaaat taactatgac agatttaata gtaatggcag tatctaaaat acttccaaat
     8701 catcaagcct taaattcagc atggacagat gagggtatat ttagatataa agatgtaaat
     8761 atagctattg cagttggttt agatgaaggc ttatatgttc cggtagtaaa aaatgcaaac
     8821 aaaaagtctt taaaagaaat agcaaaagaa agcaaagaac ttgcggaaaa agtaaagaca
     8881 gggaaactta tgccagcaga ccaagaaggt aatacattta caataagtaa tgttggaatg
     8941 tatggtataa ctacatttac acctataata aatatgcctt caagtgcaat cttaggggta
     9001 ggtgctacac aagataagtt tgttccagta aatggagagg ctaaaataaa acctataatg
     9061 aacttatcat taacatcaga ccatagagta atagatggaa cagtagctgc aaaattctta
     9121 aaagatttaa aagaattgtt ggaaaatcct ttatcaatgc ttgtataggt tttgatattt
     9181 tgttactgat ataaatagtt tgaatagatt atattattat aattgtttca gtaaatttaa
     9241 agcttttata tattaagaat tatttttaac agtaactata tgtgaatatg atttgcttaa
     9301 ttaaaatata agaaaataaa taggaggaga gcctatgtca gtagaagtaa taatgccaaa
     9361 agcaggagta gccatggaag aaggtactat agtatcttgg ctaaagcaag aaggcgaaga
     9421 agtaaagatt ggagaaccta tactagaaat aacaacagat aaagttaata tggagataga
     9481 gtcagaagga gaaggtacac ttgctgtaat catacataaa gaggaaggtg aagtattacc
     9541 agtatttaca gtaataggtg taatagcaga aaaaggggaa aatcaagagg aagtaaaagc
     9601 taaatattta tctggaaatg tttcaaaaga agatatagtt gaagagaatc aaaatataga
     9661 atttaaagaa gaaaaaataa acaaaaaaga gtgtaatcat gattatgatg tagtggtaat
     9721 aggaggagga cctgggggat atttatcagc attaaaagca gctcttttag gtggaagagt
     9781 tgctttagtt gaagaaaata tactaggagg tacttgctta aatagaggtt gtatccctac
     9841 aaagacttat ataaaaacag cagaaatatt agaagaaatt gaccaattat caaagagagg
     9901 tgtaaaagta actgtagata aagaacagga cattaagaag gccataaaat ataaaaatag
     9961 agtagttaaa aaacttacag ctggtgttgg cggattatta aaaagtagag atgtagatgt
    10021 gtttaatctt aaagctagtg ttaaagaaga gcataaagta attttatcag atggaaaagt
    10081 tttagataca gaaaatataa taattgctac aggttccaaa gtaaggatat tacctataaa
    10141 aggaatagaa tcaaatttaa taataactag tacagaagca ttagatttgg aaacagtgcc
    10201 agaagaatta gtaataattg gtggtggagt gattggttgt gaatttgcag agatattcaa
    10261 ttcaagaggt tctaaagtaa ctatagtaga aatggaagat agagtaatcc caagaatgga
    10321 taaagagtta agcgaatctt taaaatattc tcttagtaaa aaaggtataa atgtattaac
    10381 taaaaagaaa gtttctgaat ttaaagaaga aggaaataac atattagtgt gcattgaagg
    10441 tgaagagcca ataaaagcag atttatgtct atatgctata ggaagagaag ctaatctttc
    10501 tggcatagag gacttagata ttaaaataga taaaggttca atagttgtaa atagtaaaat
    10561 ggaaacaagt ataccaagca tatatgcagt aggtgatgtt actggaggtg taatgttagc
    10621 tcatgctgct tttaaaatgg gagaggtagc agcttcaaat gctctaggtg tgaataaaga
    10681 agttgactta ggtgcattac ctagttgtgt ttatacaatt ccagaagtag catcagttgg
    10741 tattacagaa gaggatgcta gaaaaaaata taatgtaaaa gttggtaagt ttaattttgc
    10801 tggaaatgga agagctttag cttctggtca agaacaagga tatgtgaagg tagtagcaga
    10861 tgctaaatat ggagaaattt taggtataca tatgtttgga tgtggtgtag cagaacttat
    10921 aaatcatgca gcatcattta aagcgttaga gatacctact gatgaagcaa gtgagttgat
    10981 atttggtcat ccatgtactt cagaagcttt aatggaagct ttggcagatg taaatggaga
    11041 atgtttacac ttacctaaaa aataaatcta aacataaatt attaatttaa gtctattatt
    11101 aatattaatt tatatcataa aattttaaaa cacttactta gccatacccc ttattatgca
    11161 aataatttat ttgcataata aggggtattt tttatggaaa aatatagaat atagaatcct
    11221 tgtagttttt gttgtacaaa aaagatagta atataaaata acattatcat tttagaatga
    11281 tttcaattgt aattaggaaa agtgattttt ttgtttttaa aaaaagtatt atttgtaaaa
    11341 tataaaaaaa aatttcttaa tatttgaata aaagaatagc ttaatttaaa tgttattact
    11401 ttataaaaaa ataataagta caaaaaaaac aatatatggc atataagttg ctatataaat
    11461 aataaggagg gaacaattat aaatgaggca aagaataaaa aacttaattg aaaatgaaga
    11521 taagaaaaat cctttaacag atgaagcgat ttcaagtagg ttaaatattc gaagagaaga
    11581 tgttaccttt ttgagaaatg agttaaaaat agaagattca agacaaagaa gaaaagttgt
    11641 tttaattaaa gctataaaag atattttaaa agaagaaaaa actataaata aaaatgatat
    11701 aacaagaaga ttaaacagca ttgggtttac agtatcaaga tttacagtca tacaatactt
    11761 aaaagagatt caagattctg gtgacctaaa acttgataaa gagagtgatg ataaaaaaga
    11821 agaaataatc aataatagag aaaataaaga tatagcattt gataaactca taggtagtag
    11881 tggtagctta agtacaatta taaaactagc caaagcagca attctttatc cacctcatgg
    11941 tcttcatact attattatgg ggccaactgg tgttggaaag agtgagttag cagagtgtat
    12001 gtataaattt gcagtagaat catccagatt ccctaagaat gcaccattta tagtttttaa
    12061 tgcagcagat tattcagaga atccacagct tttgctatca cagttatttg gacacgttaa
    12121 aggtgctttt acaggagctg atgagaacaa agaaggatta gtatcaaagg cagatggagg
    12181 aatattgttt atagatgaaa tacatagatt acctcatgaa ggtcaagaaa tactatttca
    12241 actgattgat aaaggtatgg tcagaaagtt aggagaaaca agactaacac ataaaataga
    12301 tgtaatgatt atttcagcaa cttctgagcc aatagattct catttactta atacatttaa
    12361 aagacgtata cctgtaacaa ttgaaatacc agaattagtt gcaagaccat taaatgagag
    12421 gtttgacatt ataaataatt tcttcttaat agaagctcaa agaatgggag caaacataca
    12481 tataaagtca gatgtattaa aagctcttat gctttacgat tgtataggta atgtaggtca
    12541 acttagaagt gatattcaag tagcttgtgc taggggacta ttaaaccaac ttacaaacaa
    12601 actaaaagaa gtaaatataa gcatatcaga tgtacctgga tatgtaaaac aaggtcttat
    12661 aaaaatcaga aattgtagag ggaaaataga aaattatgta gatggggatt taattataga
    12721 ctcaaatctt caaggtgaga tagaaaagaa aaatgaagat atatatactt ttccagatga
    12781 aatatacaaa cttactgaaa gaagacatat tgatttattg gaacaaggtt tagattttga
    12841 tgttataaat agaatcattg gaggagaatt agaaagttgc attcaaaaat acataaaaca
    12901 agttaaaaaa gtttctatta gtaacaatct tccagatata agtccaattg ttggaaaata
    12961 tatcgtagag ctaacttatg aagtaatcaa aatagcatca caatacctca gttcactaga
    13021 accgaatcta catttatgtt tagcagttca cctaaaagca gcttttgaga gattaaaaga
    13081 aggaaaagta atatcaaatg ctcatttgaa agaaataata ggaaattatc cagtagaata
    13141 ttctgtagca gaaaaggtag ctcaatattt aaaggaaaga tataacataa acttaccaaa
    13201 agaagaaata ggtataatag caatatattt taaaatgagt agtagaagag atatgtctga
    13261 acaaaaaaag ataggcgtac ttcttatggc tcacggaaga gtagcatctt ctgtatgtga
    13321 ggttacaaat aaactattag gaattaatca cgcaaggtat ttagatatgc catttgagaa
    13381 gaagccggaa gaaatgattg aagaagctac tacaataata aaagatattg atgaaggtag
    13441 aggtgtgatt atattagtag atatgggttc actttcatat tttggagata ttatttctgg
    13501 aaagactgga attaatataa ggacaatatc tagatttgat acattgcttt ctatagaggt
    13561 tatacgaaga gcagttttac cagatttaac aatagacgag atagttgatg aagttaaagg
    13621 tatagagcca caagtaaaag aaaaggttag taaatgtaac aatacaaaag tagattttag
    13681 aaaacctata attttaacat tatgtattac tggattaggg ggagcttgta agataaaaaa
    13741 aatcttagag gaaaatatta aaagtataac tgaaaaggtt gatatagaac ctataggtat
    13801 gattgaatat ccaagtcttg atttagagat aaaacaaata agcaatgata aaaatgtaat
    13861 cgccatagtt ggaacaattg accctcagat agcaaatatc ccttttttta gtctagtaga
    13921 cataaatgat aaagcaaagt taaatcaaat aaataaccta attgatacaa atcaaattgg
    13981 tgaaacaaat aaaaaatatg aattatcaga tttaatacat aaagaagata tatttgtgga
    14041 tttacaggtt aaatcaaaag aagaagttct aaaatatatg agtaaaaaac ttagagagaa
    14101 aggttatgtg acaaaaaatt tctataaaaa agtagtggaa agagataact tatttccaac
    14161 tgaattatca gatgaaatag caatacctca cacagatgga atagatgtaa ttaggccagc
    14221 tataagtata gccatcttaa agaagcctat tgtgtggagt aaaagtaaag taaaaataat
    14281 acttttatta gctgtagacc aaaagtgttt taatccatta agcactctac tctcatttat
    14341 agaaacagat gaatttaaga agataaaaaa cattaaagat agagatttta ttagggagat
    14401 gatattagat ggagtcaatg aaaataatta gtagcaattt aatttttaaa aatattgaag
    14461 tgtctaacaa tgaagatgca ttaaagtttt tgggacaacg tttgtttgac gaacagtatg
    14521 taaaagaaag ctacatacaa gctgttgtag caagagaaaa aaagtatgct acagggttac
    14581 caacagaaat atacggagtg gcaataccac atacagatat agtacatgta aatgagccag
    14641 gaatagcaat tggaatactg aataaacctg taaaatttat aatgatgggt acagacgata
    14701 ctgaaattga tgtaaaagtt gtatttatgt tagcagtgaa agagccacaa gaacaattgc
    14761 aactattaga aaaacttatg actatatttc aagataagaa tatactaaat aatattgttg
    14821 acttaagtga agaaagtgtg agtgatttat taaactctaa attgaaaaat taaacatgta
    14881 aagtatatta aaaatgtaaa aagattatta aagatttcta actatagata tctttaaaat
    14941 aaaataattt caatgggggt gactaatgtg gctaaaaaga tattagttgc atgtggaaca
    15001 ggagtatgta cttcaacaat tgctataaac aaattaaaga aagcacttca agacattggt
    15061 aaattggata tggttaatat tacacaatgt aaagtagtag aagttgcatc aaaagcacct
    15121 gattatgatt taataatttg tactacacaa gtatctagta gtataaaaac accagtgata
    15181 aatggcttac cttttttaac tggtgtcgga atggataaat taatcaatga tgtattagaa
    15241 gagttagaat tataattaaa aaaattaagg aacatggtct ctaaaatcat aagttttaga
    15301 gacttaaaat taaataatgg agggtcttta tatggagata ttaaatttca ttgttggtct
    15361 aggtgcacaa gtaatgatgc caataataat atgtattttt ggtttgatat taggtacaaa
    15421 actaggaaaa tctttaagag ctggtttaac tgtgggagtt gggtttatag gtcttaacac
    15481 tattatagct ttgctaacag ataatttagg tccagctaca caacaaatgg ttaagaattt
    15541 agggctaagt ttatcaataa tagatgtagg atggccagca gcatcagcta tagcttttgc
    15601 atcaacagta ggggctttaa ttattccaat aggtcttgtg gtaaatatag ttatgctaat
    15661 tactaatact actcaaactg ttgatgtaga catatgggat tactggcatt ttgcttttac
    15721 aggagcttta gtggcagggg ctactcaaag tgtaatgtgg ggagtatttg cagcagtagc
    15781 aaatatggta attgtattgg taatggcaga tttaactgct ccaggtatag aggaatattt
    15841 aggtatgcca ggtatatctc ttcctcatgg atttacacag gcatttgttc caattgctat
    15901 agtagtaaat aaattattag acctaatacc aggcataaat aagattgaga taaatgctga
    15961 tacattgcag aaaaaatttg gattatttgg agaaccttta ataatggggt ctgtaatagg
    16021 tgtaataatt ggtattgctg cgaaatatga tataaaagga atattacaat taggtgtaac
    16081 tatgggagct gtattaatat taattccaaa gatggcagca ttattaatgg aaggattatt
    16141 accagtatca gaagctgcac aggaatttat tgagaagaga tttaaaaaca gaggcaagat
    16201 ttatataggc ttagattcag cagtaggaat aggtcatcct gttacattat cagttgcact
    16261 agtgttggtt cctttgacta tacttatagc agctatattg ccaggaaata aagttttacc
    16321 atttgcagat ttagcagtaa ttccatttgc acttgtatta atcgtaccaa ttacaaaggg
    16381 aaatgtgttt agaacattaa taataggaat aattataatt acttctggat tattaatcgc
    16441 aactaactta gctccattat ttacccaaat ggctttaaat gctagtttta aaatgcctga
    16501 aggagcaact atgatttcaa gtatttgtga tggtgcgaat ccattaagtt gggtatttgt
    16561 aaaagttatg aactataaag ttattggagg agtagttttt ggagttatag ctttaggaat
    16621 ggctatttac aataggaata gaataataaa tgaaaataaa aagctatcta tagaagaata
    16681 aggctaaaac aattttatta aggagtgtat aggttatgaa aaaatataaa tatgattcta
    16741 aaagatactt actcagtgtt acaataccag gattttttat gattttaata ttgatttatg
    16801 cactgtttaa taactataca aattttaatt tgaatatata tagtttgtta ataattgtct
    16861 gtacttatgg tatattcaat acttttatat catcaagtaa tcctaaaaaa attattattg
    16921 atagcaaatg tatacatttt acctcattta tgactacaca taagtatgaa attaaagata
    16981 tagaaaagat aagaataaga gaattttata ataaaaaaat ttatgtaaaa attaatgatg
    17041 gaaacctttt taaaggtagg tattggataa gaacaaatat gtttaataat agtatagcat
    17101 tatatagcta tttcattgag ttggaagagt gcttatatcc agattcacta aaatttagaa
    17161 ataaaaaatt tgagaataag aacatataaa aataaaaaat tgaattgaag cctagcttaa
    17221 tcttttttga taagatataa gtttgaactt tatactaaat ttttagaatt ttcaatcaag
    17281 acaataacat aatagctaag caattagaac acaattaaga tgtataaggc atgtatatga
    17341 tgaatgggag gatttaatta atgttattag agaaattaag aaaagaagta ttacaagcat
    17401 ctttagacct tttaaactat aatttagtga cattaacagg aggaaatgta agtgggagag
    17461 atgaacaaac tggctatata gcaatcaccc caagtggaat ggattataga aatcttacac
    17521 caagtgatat tgttatagta gatgtagatg gaaatattat agatggaaaa tggaaagcat
    17581 cagtggattt atcagaccac ttgtatatat ataagcatag agaaggcata aatagtataa
    17641 ttcatacaca ttctacatac tcaagttgtt ttgctatttt aaatgaacct atagaatgtg
    17701 caagtacaac tcttgcaaat gaagtagggg gttcagtacc agttgcaaaa ttttcaccac
    17761 caacatctaa aaagatggga aaatgtgtta ttgaagcaat tggtgacaaa agagcatgtc
    17821 ttttagcaaa tcatggcgta attgcagttg gtccatctgt aggacatgct cttacagccg
    17881 ctgtaatgtt agaagattca gcaaaagtat attacttagc aaaatcaatt ggcacaccag
    17941 tgctactgcc tgatgaagaa attcaaagag cgagagatgt attttttaat gtctatggtc
    18001 aggataaata aattagataa aattttaaaa gtaatatgaa aatatagata ttcaatgata
    18061 ttaaggagat ttataatgat ataataagtt taatagttaa gaagtaaaat atgaaatgta
    18121 atagtatata aattcattta agactaaaaa gaataagata aaataaaaag gagtctgtat
    18181 ttctatgtat tacttattaa acaaaagcac aaatccttac tttaatttgg cattagaaga
    18241 atatctattt ttaaatgata aatataatga tgatattata atcatatgga gaaatgaaga
    18301 atccattttt ataggtaaaa atcaaaatcc atatcaggaa gtatattatg atgttattga
    18361 aaagggagaa attcctattt taagaagaat atctggagga ggaactgttt atcatgactt
    18421 aggtaatatc aatatgtcat tcatacaaaa aaataggcaa cttcatgaga tagatttttt
    18481 ggagcacact aagtttatgc aaaagatgct ttcaacctta ggactagatg tatctataac
    18541 tgaaagaaaa gatttatttt taaatgggaa aaaaatttca ggttctgcac agtctataaa
    18601 aagaaaaaat tctctttatc atggaactct tctttacgat tcagatttaa ataaattaac
    18661 taaatattta aattcaaata aagcaactga atcaaatgca actaaatctg tttcaagtaa
    18721 agttacaaat attaagtttt tactcgaaaa agatataaat tactttttag attattgtgt
    18781 tgaatattta aaatctaata taaagaatat aaaagaactt gaattggatg aagaagatat
    18841 taaaagtgta tacaatatag gagaagataa atataaaaag atagagtgga catatggtaa
    18901 aacgccgaaa tttcaagtag tgctaccatt taataatgaa agtaaaataa atatacatgt
    18961 taacagatgg aaagtatcaa aattttttat ttcttatgat gataatgtaa ttaatatgga
    19021 caagttgtta gatttgaatt tctttaaaga agaaataaaa gagagtattt tagaaaatta
    19081 tccacattac atagatttaa tcaatttaat attttaatat aaaacctcta atttgataaa
    19141 attgtcttat atcaaactag aggtttttaa tattcttgca tattaacaac cattacaaaa
    19201 ttgtaaaatt aaaaacaaca ctattgaaaa atcaatatat attgtaataa atatatgtgt
    19261 ttttcattag caaatagaca ttaactattt atacaactga aaatttaata tataattaat
    19321 cagatggtta aaataaaaat attaaaagtt ttaataaatg atggttaaaa ataaaatacg
    19381 aggtgatacc atgtatagtg ttataattgt tgcagcggga agcggaagga gaatgaactt
    19441 agatataaac aaacagttca taaaattaag agaaaaagaa attattgctc atacaattca
    19501 agttttttat gaaaatataa atatagatga aatagtggta tgtataaaaa aagaagaaga
    19561 agattttttt aaagaaaata taataaataa gtataacttt aaaaatataa aaatagcata
    19621 tggcggaaaa gaaagacagg actcaatata taatggttta aaaaaattag atgaaaattg
    19681 tgatatagta cttatccatg atggagcaag accatttgtc gaccatagaa taatcaatga
    19741 atctataaag gtagctaaag aaaaaaaggc tgtggttgta ggtgttccag taagtgatac
    19801 tataaagata gtgtcagatg gaacagtaca agaaactcca gaaagaaatt tgctgtgggc
    19861 agcacaaacc cctcaaacct ttgaatataa tctcataata gatgcttatg aacaagcata
    19921 taagaacaat tattatggta ctgatgattc aatgttggta gaaaatatag gtcaaagtgt
    19981 cactatggta atgggttctt atgaaaacat aaaaataact agtccagaag atttaaatat
    20041 agcagaacaa atattaaata tggaaaaaag agatgaagtt aacagtagaa ggagaattat
    20101 atgagaatag gattaggata tgatgtacac aaattaacag aggatagaaa acttataatc
    20161 ggtggtgtag agataccaca cgataaaggt ttgttgggtc attctgatgc tgatgtattg
    20221 atacatgcaa taatggattc tattttaggt gctctagcat taggagatat agggaagcat
    20281 ttcccagata cagatgaaga atataaggga gcagatagta tgaaattatt agaacatgtg
    20341 tataatctaa taacaagtaa aggatataaa attggtaata tagacagtac tataattgct
    20401 caaagcccta agatggctcc atatattgag agtatgagaa gtaatatatc aaaagtttta
    20461 aacacagaca ttgataatat aaacataaag gcaacaacag aagaaggtct tggttttaca
    20521 ggagcaaaac agggaatagc ttcacaaagt atttgtttat tattgttgac aagtcagaat
    20581 aattaatata ttattagtaa taagtttaat ggatatcaag taataggcct atatagaata
    20641 tctataatgt atgaatttaa gtgaaattaa tataaatata aaattagcta tgataaagaa
    20701 atagtaaaat gatgaaatct tcacagagag aaaacggtgg tgagagtttt caagaggaag
    20761 tctttgaacc tgtcttttag cttaaaggta taaaaatctt tacggtagga accgttatat
    20821 tcccaaaaga gagctattca tatagctaac tagagtggta ccgcggaaat taaacctttc
    20881 gtctctatac ttgtatagag atgagaggtt ttttatattt tcaggaagtt aatattcagg
    20941 aggaatctat taatgaaaat gtcaaaaatg tttatgccga cattaaaaga aatcccagca
    21001 gatgctgaga ttacaagcca tcaattaatg gttaggtcag gcatgataaa aaaaatgact
    21061 tcaggtgttt ataaccaatt accaatgggg ttaagagtgt tcaaaaaaat tgaacaaata
    21121 attagagaag aattaaataa aaaagattgt caagaaatac tttgtgcagc cttacttcct
    21181 tctgaattat ggaaagagtc tggaaggtgg actgctatgg gtgaagaaat gtttagacta
    21241 aaagacagaa cagaaagaga gtactgtctt gggccaacac atgaagaagc attcactgat
    21301 ataataagac aagaaattac ttcatataaa caattgccac taaacttgta ccaaatacaa
    21361 gtaaaatata gagacgagag aagaccaaga tttggtgtta tgagaactaa gacttttaca
    21421 atgaaggatg cctatagttt tgatgtagat gataaaggtt tagataagtc atatcaagat
    21481 atgtttgatg catatgtaag tatatttgat agatgtggat tggagaatag ccctgtacag
    21541 gcagattcag gagctatagg aggttctaca tcagccgaat ttatggttaa gtcagaagtt
    21601 ggtgaagatg aagttgtatt ttgtagtgga tgtgattatg cagccaatgt agaaagagca
    21661 gaatcttgta atctagcatc acaaaaagaa gaaatgaaag aattagaaga ggttcataca
    21721 ccaggagcag ctacaataaa agagttagaa gaatttttaa aaacttctcc agataagttt
    21781 gctaaaactt tagtatatga ggcagatgga aaaacagttg tagtcgttgt aagaggagat
    21841 agagaagtta atgaaatcaa agtatctaat gccataggtt ctgttataga atttgctctt
    21901 gctacagatg atgtagtgag aaaagtaact aatgcagaag taggatttgc aggtccaata
    21961 ggtataaatg ctgactatgt atttatagat aaagaaattg ttgaacaaag aaacatagtt
    22021 gttggggcta ataaaactga gtaccatata aaaaatgcta actatggaag agattttgaa
    22081 ggaatagttg gagattttag aaatgttcaa gaaggcgaca agtgtatagt atgtggtaag
    22141 ccacttgaaa tagctagagg tgttgaagta ggacatatat ttaaactagg aactaagtac
    22201 tcagaatcta tgaatgcaaa cttcatagac aaagatggaa aatcaaagcc tatagtaatg
    22261 ggatgttatg gaataggtgt tgaaagaaca gcagcagcta taattgaaca acataatgat
    22321 gagaaaggta taatatggcc tttgtcagta gctccatacc atgttgtgat agttccagca
    22381 aatatgaaaa atgaagaaca aatatcaata gctgaaaata tatataatga tttgcaagct
    22441 atgggtgtag aagttttatt agatgacaga gatgaaagaa taggtgtcaa attcaatgat
    22501 tcagaattaa taggaatacc tatgaggatt acagtaggta aaaatattaa tgaaggtaaa
    22561 gtagaattta aacttagaca taaagaagat aaagagataa ttgacataga agaaataaat
    22621 gaaaaagtaa aggcagagtt tatcaggaat aatgttaggt taggataata acttaaataa
    22681 gatattgtat agataatcaa agttgtactt tatagacacc attttatcta tatataagga
    22741 tataaaatgg tgttttttta taaaacaaat aaatgaggag gtaattgaaa tggcaaaaaa
    22801 tgaaaaacaa tttgttgaag aaataacaaa gatggaagat gattttccac aatggtacac
    22861 agatgtaata actaagactg acttagttga ttatgcacca gtaaaaggtt ttatggttgt
    22921 aaagccatat ggatatgcat tatgggaaaa gatgcaagaa tttatggata aaaaatttaa
    22981 agaaactgga cataaaaact gttattttcc attattaata ccagagagtt tattaaacaa
    23041 agaagcagaa cacgttgaag gttttgctcc agaagttgca tgggtaacac atggaggaaa
    23101 taaaaaatta gaagaaagat tatgtgtaag acctacttca gagactataa tatgtacaat
    23161 gtatgcgaaa tggttaaaat catatagaga attaccatat ctttataatc aatggtgctc
    23221 agttgttaga tgggaaaaat caactagacc tttcttaaga acatctgagt ttttatggca
    23281 agaaggtcat acattacatg aaacagcaga agaagcacaa gaagaaacaa tacaacaatt
    23341 agaagtatat aaagcgctat gtgaagaatt acttgcaatg ccagttgtag ctggtcaaaa
    23401 aagtgaaagt gagaaatttg caggtggtga aagaacatat actatagaag ctatgatgca
    23461 tgatggtaaa gcattacaat caggtacaag tcacttccta ggtcaacact ttactaaggc
    23521 ttttgatatt acttttgcag atagggaagg aaatttagct aatccatacc atacatcttg
    23581 gggagcatct acaagactta taggtggact tataatgact catagtgata acagaggttt
    23641 agtattacca ccaagagtag ctccaattca agttgttata gttcctatag ctgctaaaaa
    23701 aggaaatgta atggagactg ttgataaaat atatgctgat ttaaaagcta agggagtagc
    23761 tgtagaagtt gatgatagag ataactatac aactggatgg aaatttaatg agtgggaaat
    23821 gaaaggtgtt ccagtaagag ttgaaattgg accaaaagat atagaaaata atcaagctat
    23881 ggtatttaga agagatactt tagaaaagga ttctatgcct ttagaaggat tagctgatgc
    23941 tatatgtgat ttatttgatg ttatacacaa tgatatgttt gaaaaagcaa gaaaacatag
    24001 agaagacaat acatctatag ttgaaaatat ggatgagttt agaaaagctt tagaagaaaa
    24061 gccaggattc ataaagacta tgtggtgtgg agatgcagaa tgtgaagcta agattaagga
    24121 agaaacagga gcaactataa gatgcttacc ttttgaacaa gaaaacttag gtcacaagtg
    24181 tgtgtactgt ggaaaagaag cagatagtat ggtagtaatg gcaaaagctt actaaaatac
    24241 atttaaataa aaactggggt agaaagtatt taatacttta tcccagtttt ttgtttttta
    24301 tgagtaataa aatgtatttg taactatgtc ataagataag tcatagatac ttatattaaa
    24361 aagaatataa tagagaacta tttttaataa ttacatatta atgaaagcaa aatattttgt
    24421 tatatagatt ttcaataaag caaacaataa atataaatga atattaataa ggagtgcttt
    24481 taatttttag aaaataaatg acttaaaaga aagatttatg aaattaaaac taggattttc
    24541 tgatataata aacatgtgta tttttaaata aaaaggatat tattattaga tttgattaaa
    24601 aataaacttg caaaacaatg tactagttta atattatagt tatattgaat aaaatatcag
    24661 gaggaagtaa aaatgaatgt aagagtaagg tttgcaccaa gtccaacagg atttgtacat
    24721 ataggtagtt tgagaactgc tttatataac tatttatttg caaaaaaaat gggcggagaa
    24781 tatatattaa gagtggaaga tacagaccaa agtagattag tagaaggtgc tatagagaat
    24841 atgcttaatg ctatgaaatg ggctggggtc aaccatagtg aaggtgttat attagatgat
    24901 agtggaaaag tagtacaaaa aggagaatat ggtccatata ttcaatctca aagattagat
    24961 atataccaag aatatataaa gcaactttta gatagtggaa aagcttatta ctgtttctgt
    25021 acaaaagaaa gactggatga agtaagagat gctcaaagag cagcaggaga aacagctaaa
    25081 tatgatggtc attgtaagaa tctttctaaa gaagaagtag aagcgaatat aaaagcagga
    25141 ataccatatg ttataagatt aagattgcca gagaatcata caattaagtt tacagactta
    25201 gttagaggag atatggaatt taatactaat gacttagatg accaagtatt aatgaaaaca
    25261 gatggatttc caacttatca ttttgcagta gttgttgatg actatttgat gaaaattaca
    25321 catgttataa gaggagaaga atgggtatca tctactccaa aacatgttta tttatatgaa
    25381 gcttttggat gggaagcacc tgtatttgtg catttaccaa atatacttaa taaagaaaag
    25441 aaaaaattaa gtaaaagaca aggtgatgta gctgttgaag actttaagaa aaaaggatat
    25501 ttaccagagg gattagtaaa ctatgtagca ttagttggtt ggtctcctga agataatcaa
    25561 gaattgttta caatggagga attagaaaaa gccttctctg tagaaagagt ctctaaaagt
    25621 ggtggagtat ttgatactga aaaattaaat tgggttaatc agcattatat aaaagatggt
    25681 gatgatgctt atctaacaga cttagcaata ccatttttaa tagaagatgg atttataaca
    25741 gaagaagaag ctactaataa atatgatttc ttaaagagta tgatatcagt acttaaagag
    25801 aaattacaat atgtaaaaga agttactgaa catgcaagta tattttttgg agataaaata
    25861 gaggtagaaa ctgaagaggg aagagagttc ttaagattag aacatatacc tactttaata
    25921 gatgctttga gagaaaaaat agaaaaaaca gaggtattaa atgctgagtt tgttcaagct
    25981 atgcttaaag aaatacaaaa agagtacaaa ataaaaggga aaaatttatt tatgggctca
    26041 agaataattt tgacagggca aatgcatggt ccagatttac caaaagtaat ggaagtttta
    26101 ggaaaagaaa cttgcttaaa tagaatagct tatgtaaaaa ataatatatt atagtcataa
    26161 aagtccgcta atttagaagc ggactttttt tgtgttagta tttggtgaaa atatctgctt
    26221 aagaaaatga aggtatttaa ggtaataaat ttaatatata aaaagcaaag caaaaatata
    26281 aataaaatat gaataataaa ctaataaaat attaagcata gaagaaggga atctattatt
    26341 ttagtttgat ttaattatta agggggtaat tactatgtat agtaatattg atgatgttaa
    26401 aaaggaatta aaggagctat gcttagaata tgttacaatt ttagaaaaat taaaagatga
    26461 aaaaatgatt acagaagaaa cttttgaaaa gtgctcttct caaaaaaaaa tatttttaga
    26521 agaacaatag aggatggtaa tagtattatt attatgtagt ttgaaagata gtacgaaaaa
    26581 tgatagtaat actattattt attgcaattt tcaactataa tataatatat aatgttaaga
    26641 agataattta taaaaataca gtgaggtgat gagtgtgaaa gtttataata cattgactag
    26701 aacaaaagaa gaatttgttc cattagaaga aggaaaggta aaaatgtatg tgtgtggtcc
    26761 aacagtttat aactatatac acataggtaa tgcaagacca tttataatat ttgatactct
    26821 aagaaggtat ttagagtata gaggatatga tgtaacttat gtacaaaact ttacagatgt
    26881 tgatgataag attataaata gaagtcatga ggagggaata tctcctgaag aagttgcggc
    26941 taagtatata aaagaatatt ttgtagactg tgatgggcta ggaataaaaa gagctactgt
    27001 tcatcctcag gttacagata atatacaaca gattatagag tttataaaag agttggaaga
    27061 taagggatat gcatatgcag taaatggtga tgtatatttt gatacaaata agtttgaagg
    27121 ttatggaaaa ctttcaggtc aaaaacaaga agatttagag gcaggggcta gaattgaggt
    27181 taatgaccaa aaaagacatc ctatggactt tgttctttgg aaggcaaaaa aagaaggtga
    27241 gccaggatgg gatagccctt ggggagaagg tagaccagga tggcatatag aatgttctgt
    27301 tatgtctaag agatatttag gtgaaacaat tgatatacat gcaggtgggc aagatttaac
    27361 attcccacat catgaaaatg aaatagctca aagtgaagct agaagtggaa aaacattctc
    27421 aaaatactgg atgcataatg gatatataaa tataaatgat gaaaagatga gtaaatcaaa
    27481 aggtaatttc tttacagtaa gagatatatc aaaattatat gatttagaga tagtaagatt
    27541 tttcatgtta tcagcacatt atagaaatcc agtaaacttt agtgatgaga tgttaaatca
    27601 agctaaagca ggtcttgaaa gactatataa tactaaagaa aaattagaat ttacgctaag
    27661 taatttagta gaatctcctt taacagaaaa agaagtagag cttgttaaag aattagatga
    27721 ctttagacaa aaattcatag atgctatgga tgatgatgtt aatacagcag acgcagtaag
    27781 tgtaatattt gaattagcta agttaataaa ttctaatgta gatgaaaatt cttctttgga
    27841 gtttgctaaa aaatgcttag atgaatttaa tgaacttaca ggagtattaa atattgtaaa
    27901 taagaaaaag gatactgtat tagataaaga cattgaagaa ttgatacaaa aaagaactga
    27961 tgcaaagaaa aataaagagt ttcaattagc tgatgatatt agacaacaat tactaggtat
    28021 gggaattgta ttagaagaca caagacaagg tgttaagtgg aaaaggatat aaaaatggaa
    28081 aaaactgaat tagttactat gtcaccatta gttttagcat acttaggtga tacagtgtat
    28141 gagacatata taagagaata tttaattaga cagaatacac aaagaaaagt taatgatttg
    28201 cataaactag ctataaagta tgtaaaagct aaagctcagg caactataat tcatgaaatt
    28261 gaaattgagt taactgaaga agagagtaaa atttataaaa ggggaagaaa tcaaaagtca
    28321 aatacttctc ctaagaatgc agatattata gactacaaac atgcaactgg gtttgaggca
    28381 ttagtgggat atttgtattt aaataatgaa atagagagac tacaatatat tatcaataaa
    28441 ggaatcaaaa ttattgaaag agatatgtaa atattaaaga gtgttgtctt aataataagt
    28501 aataaggcaa cacttttttt ataactaatg tataataaat atatagaaat tagtaatgag
    28561 aaaggataaa aagttatgaa agtaaaatta atatcacata cacctgaacc agaaaaagta
    28621 atagctatgg cagctaagct atgttattca ccagttggga cagacgaaat agaaaaagac
    28681 ttaacagatg aaagtataga gaaattttta aatatgttat taagtatagg tcatgggtca
    28741 atattagaac atgcttcatt tacattttca atagaaggta tttcaagggc ttgttcacat
    28801 caaattgtta gacatcgtat agctagtttt tcacaacaaa gtcaaagata tgttaaatta
    28861 gaacaatttg agtacataat tcctccagaa atagaaaaaa tagaaaaagc aaaagagtta
    28921 tttatagact ccatgaaaaa agaccaagag aattatgata agttagttga aatattgttt
    28981 gaaaatcatt ataatgacct aataaaaaac ggtaaaaatg agaaaacagc taaaagacaa
    29041 gctgaaaaga aagcaataga agatgctaga tacgtatttc caaatgcatg tgaaacaaaa
    29101 atggtattta ctataaatgc tagaagtttg tttaatttct ttgaacatag atgctgtgaa
    29161 agagcacagt gggaaataag aaatttggct gtggagatgt taaaagaggt taaaaaggtt
    29221 gctcctatat tatttaaaaa aactggtcca agttgtgtaa atggaagttg tccagaagga
    29281 actatgactt gtggagatat agttcaagtt agagaaaaat ttaaggcctt gtaggaggtg
    29341 aataatttgg cgagtataga aggaagaaat ccggtaatag aagcaataaa aagcgacaga
    29401 gaaatagata aaatattaat tgcaaattca gctaaagaag gttcaattaa gaaaataata
    29461 ggaatggcta aagataagaa tataattatt cagtatgttg ataaacataa gctagatgaa
    29521 gtaagtacaa gtcattcaca tcaaggtgta atagcttatg caagtgagta taagtattat
    29581 gaattagatg agctaataga tttagcaaag aataaagatg aagacccatt tttcattata
    29641 ttagatgaga taacagaccc acataatttg ggttcaataa taaggacagc agatgcagta
    29701 ggagcacatg gagttatcat tccaaaaaga aggtctgtac acataactcc tgtagttgcc
    29761 aaagcatctg ctggagcagt agaatatatg ccagtttgca aggtaaccaa tatagttaat
    29821 acaataaaaa gactcaaaga agaagggctg tggattgctg cagctgatat ggatggagaa
    29881 actttttata aacaaaatct tactggtcct cttggtgtag taattggaag tgaaggtttt
    29941 ggaatatcta gattggttaa acaaaactgt gactttatag taaaaatgcc aatgataggt
    30001 aatgttacat ctttaaatgc atctgtagca ggtggaatac ttttatatga gatattcaga
    30061 caaagactgg ataaaagtaa ataggcatga aaaaaaatat aaatcattac ttaataatag
    30121 atggctacaa tattataaat gcgtgggaca atttgaaaga actggctaaa gaggacttag
    30181 aagattctcg agaaaagctt attgatgaca ttatagaatt ttctgagttt atgggttata
    30241 agactataat agtttttgat gcttataatg ttaaaaattc tcgagaaaaa gttgaaaaaa
    30301 gaaaacacat aacaatagtt tatacaagag aacaccaaac agcagatagt tatatagaga
    30361 aatttataac ttctttatca aagtatgatg atgtaaaagt agccactaat gactatgcag
    30421 aacagcaaat gattttagga aaaggtgcta ctagaatgtc tgcaagagaa ttaaagttag
    30481 agttagatcg ttccaagaat aaaatgaagg aaaaaaatat cggtttgagg aaaaaaattc
    30541 agcgaaattg gttggaggaa aggttagata aagaaacatt gtcgaaactt gagaacattc
    30601 gtagaaagcg ttgaaattac taggttcttt agactataat atacctataa atttgtttta
    30661 tatatggggg agatttacat gttggtagca aaagaaaaaa gttatgagtt agtggacaat
    30721 tgtcagcagg atgagtataa cattgtatta agagcaagtg aaggggataa aatagcacta
    30781 gagtacatta ttactaaata cagaaatttt gtaaaggcaa aagcaaagtc atatttttta
    30841 attggagcag ataaagaaga tataatacaa gaaggaatga taggccttta taaggcagtt
    30901 agagattttg atggaagtaa gaccaattca tttaaatgtt ttgcggagat atgtataaca
    30961 agacaaataa taactgctat aaaaactgca acaagacaaa aacatatacc tttaaattca
    31021 tatgtttctt tgaataaacc aatatatgat gaagagtcag acagaacact tttagatatt
    31081 atagctacaa gtatagtaac agatccagaa gagcttataa ttagtaaaga agagttaaaa
    31141 aatatagaat caaaaatgaa cgaattgtta agtgacttgg aattagaagt actagaatta
    31201 tatttaaatg gaaagtcata ccagtttata gcggacaagc ttaaaagaga tgtaaagtca
    31261 atagataacg ctttacaaag ggttaagaga aaattagaaa aacatcttga aaacagaaat
    31321 gattaatagt aaaatactta ccataagctt attgtgagca aaattaattt atatcgaaag
    31381 ataagggagg aaattcaaaa tggctaaagc taaatacgaa agaacaaaac ctcatgttaa
    31441 tatagggaca ataggacacg tagaccacgg taaaactaca ttaacagcag caataacaaa
    31501 aacattatat gacagatatc aattaggaga agcagtagat ttcgcaaaca tagataaagc
    31561 tccagaagaa agagaaagag gaatcacaat atcaacagca cacgttgagt atgaaacacc
    31621 aaatagacac tatgcacacg ttgactgccc aggacatgct gactacgtta agaacatgat
    31681 aacaggagca gcacaaatgg acggagcaat attagtttgt tcagcaacag atggaccaat
    31741 gccacaaaca agagagcata tactattatc aagacaagtt ggagtaccat atatagtagt
    31801 attcttaaac aaatgtgaca tggtagatga tgaagagtta ttagagttag tagagatgga
    31861 agtaagagat ttattaacag aatatgattt cccaggagat gacactccaa tagtaagagg
    31921 ttcagcatta atggcattag aagatccaaa gagtgagtgg ggagataaga tagtagaatt
    31981 attcgagcaa atagatgagt atataccagc tccagagaga gatacagata aacc
//
LOCUS       DAGSWI010000036        31876 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.036, whole genome shotgun sequence.
ACCESSION   DAGSWI010000036 DAGSWI010000000
VERSION     DAGSWI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.036"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..189)
                     /locus_tag="MJF80_001499"
     CDS             complement(<1..189)
                     /locus_tag="MJF80_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBZ0280169.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILIS"
     gene            373..717
                     /locus_tag="MJF80_001500"
     CDS             373..717
                     /locus_tag="MJF80_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBZ0280170.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            748..2349
                     /locus_tag="MJF80_001501"
     CDS             748..2349
                     /locus_tag="MJF80_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBZ0280171.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2368..3468
                     /gene="dprA"
                     /locus_tag="MJF80_001502"
     CDS             2368..3468
                     /gene="dprA"
                     /locus_tag="MJF80_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBZ0280172.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3501..5588
                     /gene="topA"
                     /locus_tag="MJF80_001503"
     CDS             3501..5588
                     /gene="topA"
                     /locus_tag="MJF80_001503"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBZ0280173.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5753..6538
                     /gene="codY"
                     /locus_tag="MJF80_001504"
     CDS             5753..6538
                     /gene="codY"
                     /locus_tag="MJF80_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBZ0280174.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6645..7910
                     /locus_tag="MJF80_001505"
     CDS             6645..7910
                     /locus_tag="MJF80_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBZ0280175.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDVEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7941..8711
                     /locus_tag="MJF80_001506"
     CDS             7941..8711
                     /locus_tag="MJF80_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0280176.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8820..9266
                     /locus_tag="MJF80_001507"
     CDS             8820..9266
                     /locus_tag="MJF80_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBZ0280177.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9284..10477
                     /gene="nifS"
                     /locus_tag="MJF80_001508"
     CDS             9284..10477
                     /gene="nifS"
                     /locus_tag="MJF80_001508"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBZ0280178.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGIAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10531..10971
                     /gene="nifU"
                     /locus_tag="MJF80_001509"
     CDS             10531..10971
                     /gene="nifU"
                     /locus_tag="MJF80_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBZ0280179.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11260..12339
                     /gene="mnmA"
                     /locus_tag="MJF80_001510"
     CDS             11260..12339
                     /gene="mnmA"
                     /locus_tag="MJF80_001510"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBZ0280180.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12482..12720
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12788..15427
                     /gene="alaS"
                     /locus_tag="MJF80_001511"
     CDS             12788..15427
                     /gene="alaS"
                     /locus_tag="MJF80_001511"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBZ0280181.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15452..15709
                     /locus_tag="MJF80_001512"
     CDS             15452..15709
                     /locus_tag="MJF80_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBZ0280182.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15719..17890
                     /locus_tag="MJF80_001513"
     CDS             15719..17890
                     /locus_tag="MJF80_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280183.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDIMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18096..18521
                     /gene="ruvX"
                     /locus_tag="MJF80_001514"
     CDS             18096..18521
                     /gene="ruvX"
                     /locus_tag="MJF80_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBZ0280184.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18599..18886
                     /locus_tag="MJF80_001515"
     CDS             18599..18886
                     /locus_tag="MJF80_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBZ0280185.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19168..19626
                     /locus_tag="MJF80_001516"
     CDS             19168..19626
                     /locus_tag="MJF80_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBZ0280186.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19743..20480)
                     /locus_tag="MJF80_001517"
     CDS             complement(19743..20480)
                     /locus_tag="MJF80_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBZ0280187.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20697..22373
                     /locus_tag="MJF80_001518"
     CDS             20697..22373
                     /locus_tag="MJF80_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBZ0280188.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22580..22780
                     /locus_tag="MJF80_001519"
     CDS             22580..22780
                     /locus_tag="MJF80_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBZ0280189.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22834..23997
                     /locus_tag="MJF80_001520"
     CDS             22834..23997
                     /locus_tag="MJF80_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBZ0280190.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24208..24630)
                     /locus_tag="MJF80_001521"
     CDS             complement(24208..24630)
                     /locus_tag="MJF80_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBZ0280191.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24651..25487)
                     /locus_tag="MJF80_001522"
     CDS             complement(24651..25487)
                     /locus_tag="MJF80_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBZ0280192.1"
                     /translation="MRQEERTVCFDRELKIEAYTFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25785..26381
                     /locus_tag="MJF80_001523"
     CDS             25785..26381
                     /locus_tag="MJF80_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBZ0280193.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAGYVLLYSMGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGIYQIILSPIYTVLLKIVYLFV"
     gene            26411..27130
                     /locus_tag="MJF80_001524"
     CDS             26411..27130
                     /locus_tag="MJF80_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBZ0280194.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27132..27671
                     /gene="scpB"
                     /locus_tag="MJF80_001525"
     CDS             27132..27671
                     /gene="scpB"
                     /locus_tag="MJF80_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBZ0280195.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVVTNE"
     gene            27833..28462
                     /locus_tag="MJF80_001526"
     CDS             27833..28462
                     /locus_tag="MJF80_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBZ0280196.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            28416..28844
                     /gene="ytfJ"
                     /locus_tag="MJF80_001527"
     CDS             28416..28844
                     /gene="ytfJ"
                     /locus_tag="MJF80_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBZ0280197.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            29092..29532
                     /locus_tag="MJF80_001528"
     CDS             29092..29532
                     /locus_tag="MJF80_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBZ0280198.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            29548..30999
                     /locus_tag="MJF80_001529"
     CDS             29548..30999
                     /locus_tag="MJF80_001529"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBZ0280199.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVFTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNIATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(31090..31758)
                     /locus_tag="MJF80_001530"
     CDS             complement(31090..31758)
                     /locus_tag="MJF80_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBZ0280200.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFILSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT        12979 a         3262 c         5685 g         9950 t
ORIGIN      
        1 agaaataagt ataaatttat tctcatttga taaagaaaaa aatctaactt gattatttat
       61 tataattaga aataatatta tcatagttat gaccggatac aatgtatttt caacattttt
      121 caaatagctt acaactaata taaataaaga taaatatcgt ataaaagcaa ctaatttttc
      181 tctgttcatt aaaggcactc ctccaaaaaa ataaactttt gctatgaaaa ctagaataat
      241 atcataaaag aataaagtca atagaagtat ttattaatat tgatggaaat aaaatcaatc
      301 caaaaacttg aatagtaaaa ttgtatactg acaataattt aaaagtgaaa atttaatgta
      361 ttggagtgaa taatgaataa taaagaaaaa ggtgattttg gagaaaaagt agcagttaat
      421 tatttgctat caaaaggagc aaagatacta gaaaaaaatt acagattaaa gataggggaa
      481 atagatataa tagcaaaaat ggaagatgaa attatatttg tggaggtgaa atcaaggtct
      541 aacataaagt ttggctaccc ttgtgaatct gtaagcttca aaaaaagaaa aaagataatt
      601 ggagttgcaa gttattatat tataaaaaat aacttaaata acactcccat aagatttgat
      661 gttatagagg tatatttatt agaaaaaaga ataaatcaca taatgaatgc tttttaaaaa
      721 agataatgtt tttggaaagg attaaatatg ttaagtatta ttaattcaag caacttagta
      781 ggaatcgaca gctttttagt taaggttgaa gtagatgtaa gcaatggaat acctagtttt
      841 aatattgttg gtcttccagg caaagaaatt aaagaagcaa gagaaagagt aaagtcagca
      901 attcttaata gtggatataa atttccaagc acgagaatag ttgtaaattt atcaccagca
      961 gatataaaaa aagaaggagc ttttttagat ttatctataa gtataggcct tttaagagag
     1021 ttaataaaaa aagatgaaaa ttacataaga gaaagtatgt ttataggaga attatcatta
     1081 gatgggaaaa taagaaaagt aagaggtata ttgcccataa ttatgggtgc aaaaactcag
     1141 aacataaaac gtatttttat accaattgag aacataaaag aaagtctatt tgtagatgaa
     1201 attgatataa ttcctattaa aagtttaaaa gaatgtgtcg attttttaaa cgaagagata
     1261 aaagtcgata aagtaaggat aatgtctttt ttggatgata agtcaagaaa agagaacgaa
     1321 gaattagaaa aagataatag ttatatagat tgcaaatata ctaagattaa taatgaagaa
     1381 agtaaatatg atgaagactt taaagatgta aaaggaaatt attttgtaaa gaggagtgca
     1441 gaaattgctg ctgctggaaa tcataatatg tttatgattg gacctccagg ctcaggaaaa
     1501 actatgattg caaaaagagt aaggacaatt ttaccagata taagtgtaga agaaatgata
     1561 gaagtaagta aggtctatag tatattgggt atgataaatg aaactaaagg aataattgat
     1621 aaaagacctt ttagagcacc tcatcataca actacaaagc aatctttaat tggaggagga
     1681 atggatgcaa gacctggaga aattgcttta gcacatagag gtatcttatt tttagatgag
     1741 atagccgaat ttgatagaaa aatacttgag actttaagac aacctataga agatggatat
     1801 gtaaatatat ccagagtaaa gtacagtgca aaatatcctt gtagagtact gttagttgca
     1861 gctatgaatc catgtccatg tggttattat atgtcagaga cagagtgtag atgtagaagt
     1921 aacgaaattg atagatacat aaataaaata tcaggtccat tgttagatag gtttgatatt
     1981 tttgtagaag ttaattctat aaaatattca gattttaatt ctttaaaaca agaagaaagc
     2041 tctcaaaaaa taaagagaag agtagaaaat gctagaaaaa tacagataaa tagatttaag
     2101 aaagataata taaaaaataa tagtgaaatt aaagcttaca atttatttaa atattgtaaa
     2161 ctggaaaaag aagcttcaaa aacggctgag atgatattta gtaaatacaa cctaagcagt
     2221 agaagttata caaaactttt aaaaatggca agaacaatag cagatttaga agaaagagat
     2281 ttgataaatt cacagtgtat tatagaagct ttttcattta gaaaggctta ttatagttat
     2341 tttaaatagt tttttgaggt ggattttatg gaaaaaagag atacttattt atggttaaaa
     2401 tcaattggtg gaataacaac aaaaacaatt gaaataatag aaaatgaaat agtaaatatt
     2461 gaagatatat ttgatttttc tgaaaaagaa atatataatt taaaaaatat aagcttgaat
     2521 attagaaaaa atatagtaaa atatagaggt cacgcttatt tagaaaatat aaaagaacta
     2581 ctttacaaaa aagcgattaa atatatttgt aaatatgata aagaatatcc tgaaaattta
     2641 aagaatatat acaacgctcc aaagttatta ttctacaaag gtgacatagg cttagttaat
     2701 aataatttta atatagctat agtaggttca agaaaaccta cagcctatgg aattaattgt
     2761 gcaaaaacaa taagttgtca actatcccaa tatggagtta atatagtaag tggattagct
     2821 ataggaatag atgcatactc gcacataggt tgtatgagtg gaaaatctaa aactatagca
     2881 gtacttggtt caggagtaga caatcctctt ccaaaacaaa atttacattt atcaaataaa
     2941 atattagaaa atgggggatt attattatca gagtataata ttaactcgac agtagccccg
     3001 taccattttt ctaatagaaa tagaattatc agtggtttaa gtgatggagt agttgttgta
     3061 gaagcagcaa taaaaagtgg tgcactaatc acagtagatt ttgctcttga acatggaaaa
     3121 aatgtttttg caatacctgg taatataaat tctcaaatga gtagaggttg tcataaaatt
     3181 ataaaagaag gtgcaaaatt aattgaaaat atagatgata ttttaaatga atataacata
     3241 tttaatatta ttgataaaaa aataaatcaa aaatatgata atataagtct aaatgccaag
     3301 tctaagcaaa taattgaagc tataaaaaga gaaggaaact tgcatataga tagtatttgt
     3361 gattatactg gtatagaaat aaagtatgta aattcaataa tcaatgaatt agtactaaat
     3421 gagttagtag tagaaatgaa taataaaacg tacagtttaa atgtataaat taagattagg
     3481 tagtgggggt gtatggtttt atggccaaaa cattagtcat cgtagaatcg cctgcaaaag
     3541 ctaaaactat agaaaaattt ttagggaaaa gtcattacac agttaaagca tcagttggtc
     3601 atgtcagaga tttacctaaa agtaaattag gtgtagatat agaaaataat tttgaacctc
     3661 aatatataaa tataagagga aaaggcgatg taataaaaga attaaaaaaa gaagctaaaa
     3721 aatctaaaca agtatatcta gcaactgacc ctgatagaga aggtgaagct atatcatggc
     3781 atttagctca tatactaaat ttagatgaat ctgaggattg tagaatagag tttaatgaga
     3841 taactaagga tgcaattaaa aaagcaatta aacatcctag aaatattgat ataaatttgg
     3901 ttgatgcaca acaggcaaga agagtgctag acagactttt aggttatcaa ataagtccta
     3961 tattatggca aaaggttaga aaaggtctta gtgcaggtag agtgcaatct gttacaacaa
     4021 agttaatttg tgacagagaa aaagaaataa aggcatttat tccaaaagaa tattggacaa
     4081 tagatgttga ggctaagaca gtaaaagaca aagatataac tttgaaattt tatggtaaaa
     4141 atgatgaaaa aattgaatta gaaaatgaag aagctgtaaa tgaaatacta aaagaaatgg
     4201 aaggtaaaga tttaaaggtt gagaagatag aatctaaatc aagacgtaaa tcagctccaa
     4261 aaccatttac aacaagtatg cttcaacaag aaggagcaaa taagttattt tttactacta
     4321 aaaaaacaat gatgatagca caagaattat atgaaggtat cgacatagaa ggtgaaggta
     4381 ctgttggtct tatatcatat ataagaactg actctaaaag aatatctgat gaagcaaaag
     4441 aaaaagctaa gggctatatc gtaaacaatt taggagaaaa ctactataaa aacactggtg
     4501 ataaaaaaga aaaaaaggat actaaaaagg ttcaagatgc ccatgaggca ataagaccaa
     4561 cctcagttga tagaactcct gagagtataa aagcctcatt gagcaaagac cagtacaagt
     4621 tatacaattt aatttggaga aggtttgtag ctagtcaaat ggaagattca gtgtttgata
     4681 tactaaatgt agaatgtaaa ataggcgata ttgtatttaa ggcaactggt tcaaaaatga
     4741 aatttgatgg ttataccaag gtatataatt tttcagaaag agaagacaaa attttaccaa
     4801 gtatagaaaa aggagatata ttaaatatag aaaatatatt gccagaccaa cattttacac
     4861 aaccaccagc aagatataca gaagctagtt tagtaaaaac actagaagaa ctaggtatag
     4921 gtagaccaag tacgtatgct ccaacaatag ctactatact gaatagagag tatgttgaaa
     4981 agcaaggaac aagtctatgt ccaacagaac ttggaataat agttactgat attttggaaa
     5041 ataatttcca taaatttata gatgtagatt ttactgctga tatggaaagt aaacttgatg
     5101 ttatagaaga aggcaataca gattggaaaa agattgtatc agaatcatat gcaccactta
     5161 aagaggctat tgaagaagct cttgaaaata tagaaaaagt aaatatggat gaagaaactg
     5221 atgagatttg cgagaactgt ggttctaata tggttatcaa atatggtaga tttggaaagt
     5281 ttatggcttg taaaaactat cccgattgta aaacaactag accaattatg aataaaattg
     5341 gtgttaaatg tccaaaatgt gaagaaggag atattatact tagaaaatct aaaagaggta
     5401 aagcttttta tgggtgttct aattatccaa actgtgactt tgtagcttgg aataagccaa
     5461 caggagagtt ttgtgagaag tgtggttcat atatggttga aaaagtgact aaatctgaaa
     5521 caaaaacagt gtgttctaat aaagaatgta ataaagagac aaaagagcat aatgacggaa
     5581 aagagtgaaa attgaaaaaa atcacataat tctcttgaaa aaggttccaa tctatgataa
     5641 tctttcaagt gtaaacaact aatagaaaaa taaattttca ttaaatgtga aaataatatt
     5701 agaaaatata aaaggttatt aaaaggccta aatttcatga ggagatgatt aaatggcaag
     5761 tgaagtgtta caaaaaacaa ggaaaataaa taaaacatta caaacaagtg gtggaagcag
     5821 tgtctctttt gatttactgg ccggagcatt gggcgacgtt ttaagttcta atgtttatgt
     5881 agtaagtgca aaaggtaaag tactaggtct tcatttaaat gatgttcaag acagttcagt
     5941 tatagaagat gagtatacta agcaaaagaa attttcagat gaatatactc aaaatgtgtt
     6001 aaaaattgat gaaacattag aaaatttaaa tggtgagaag atattagaaa tctttcctga
     6061 agaacatgga agattacaaa aatatactac agtagttcca atattaggaa gcggtcaaag
     6121 attaggaaca ttggtacttt caagatattc aaattcattc aatgatgatg atttagtaat
     6181 agctgaatac agtgcaactg ttgttggtct tgaaatatta agagcaatag gtgaagaatt
     6241 agaagaagaa atgagaaaga aagctgtagt tcaaatggca ataggcactc tgtcctactc
     6301 cgagcttgaa gcagttgaac atatttttgc tgaattggat ggaaaagaag gtctacttgt
     6361 agcaagtaag atagctgata gagttggtat aactaggtct gtaatagtaa atgcacttag
     6421 aaaatttgag agtgcaggtg tgatagaatc aagatcatta ggtatgaaag gtactcatat
     6481 aagaatactt aatgacaaac ttacagatga attaaaaaaa ttaaaaaaca atcaataatc
     6541 tatattttat aggtttagat tagaaaaggt atctatttga aatagatacc ttttttagac
     6601 gaattattaa ggtaatgata agtatattaa aaaggagtga gaaaatgcct ttagcagata
     6661 aaataagacc aaaaacaatg aatgatgtaa taggtcaaag tcatataata ggtaatggaa
     6721 aaatattgtc taagatatta caatctaatt ttttaccaaa tatgatattt tttggtcctc
     6781 ctggtgttgg taaaactaca gtagcggaaa taatagcaga aagaagcaat aaaagcttct
     6841 ataaaataaa tgctactaat tcatctttag aagatataaa aaaggtgata tcagagcttg
     6901 gaagtataaa taatgtaaat ggagtcctat tatatataga tgaaatacaa agttttaata
     6961 aaaaacagca acaatcaata ttagaattta tggaaaatgg gagtataact ttgattgcta
     7021 gcactacaga aaatccatat cattatgtat ataaagctct tttaagtagg tctactgtat
     7081 ttgaatttaa acctttagaa aaacttgatg tagaaaaagg actgaaaaga gctgttgaag
     7141 tattaaatga agatagctat atggatattg agtgtaataa tgatgctatt gaagatatag
     7201 caattctttc tgatggagat atgagaagag ccttaaatat tttggaagtg gtagtctatt
     7261 caactaaacc aaataaggat aatataactt atatagattc agatgtaata agggcatcta
     7321 catttaataa aattataaac tatgacaaaa atggtgatag tcattatgat attttaagtg
     7381 catttcaaaa atctataaga ggaagtgacc cacaggcatc tatacactat ctagcaagac
     7441 ttataaaagg tggggatttg ataagtatat gtaggagact tttagtaata gctagtgaag
     7501 atataggttt agcttatcca aatgctattg taattgtaaa ggcatgtgta gactcagcta
     7561 tgcaacttgg atttccagaa gcgaaaatac cattagcaga agctactatt cttttggcta
     7621 catctccaaa gtctaattct gcatgtatag caataatgaa agctatggat gaactggata
     7681 aagagtttgt taaggatata ccaaatagta taaaggatgc tcactattct ggttctaaag
     7741 aaataggtag gggttgtgga tacaaatatc ctcataattt taaaaatcat tatgtaaagc
     7801 aacagtattt accagatagc ataaaagata gtatttatta cataccacaa gaaaataaaa
     7861 ctgagaaaaa tataaaaaaa tatttagaat atctaaatag tgatttttag actgaacttt
     7921 atgtatcggg ggtagatatt atgattataa aacttaactc aacttatcac agtagagtta
     7981 tgaaatactt aaaaaaagaa cctgagtaca atttgtttat aataggggat attgaaagat
     8041 atggatatgg aaataatttt ttaaatatat gggcagatgt gggtgaacat ggtgaaataa
     8101 aggcaatatt acttaaatat tttgaattta tgatgtttta ttctgatgga gagtatgatg
     8161 ttgaaggttt ttataatcta cttcgtaata ctaattacga agaaatatct gggaaaatat
     8221 gtgctgtaga tgctttagca aagagattag gacttaataa tttaaaggta gttgattttt
     8281 gcaaacttca aactaaaaag tttttgatag ataataattg taatgcaaag gttaagagaa
     8341 ttaggcttgg aaatctaaaa aagacagtga aactttatga cttaatagat gaatttcaca
     8401 gtacaacttt agagaattta aaaaatggac taaggacagg tagaggatat tgtatagaga
     8461 ttaacaaaca ggtggtgtca atggcaaaga gtacttcaga aaataggact catgcaatga
     8521 taattggagt aggaacacat cctaaatata gagctaaagg acttgctaca aaatgcctaa
     8581 ttaaactatg ttcagagtta ctgagagaaa acaaaattcc ttgtttgttt tatgataatg
     8641 aagaggctgg aaagatatat aaaaaactag gatttgaaaa cattggaaaa tggggaattt
     8701 attcaaaata aaaaattaaa attaatcttg acaaaattag tagggtataa taaaattatc
     8761 ttgaaagtaa ttcctactaa aacagtatga attagatttc tatatagagg tgataatata
     8821 tgaagttgtc tactaaaggt agatatggac taaaagcaat gtttgaatta gcattaaatc
     8881 aagacaatgg accagtatca ttaaaattta ttgctaagaa gcaaaaaata tcagatcagt
     8941 acttagaaca aatattttca tctttaaaaa aatcgggtct agtaaaaagt gtgagaggtg
     9001 ctcaaggagg ttatttgctt tcaaagaatg ctgaagacat aacagtagga gatatacttg
     9061 tagtattaga aggaccagtt gcattatctg actgtgtact agatgaagat gtatgtgaaa
     9121 actcaaacat gtgtgtaaca aaaatagttt gggaaaagat gaaaaagggt atagaagatg
     9181 ttatagattc aattactctt aaagatatga taaatgacta taacaaaaat aaattagaaa
     9241 atgatataac aaatattaaa aaatagattt ggagatgaat gtaatggaaa aaagaagatt
     9301 gtatatggat tattctgcaa caacacctat taaaaaagaa gtattggatg caatgatgcc
     9361 atacctaaca gattattttg gtaatgcatc tagttttcac acattcggaa gagaagcaaa
     9421 agatgctttg gataaagcaa gagaacaagt agcagccctt ataaatgctg aacctagtga
     9481 aatatatttt acagcaggtg gttcagaaag tgataactgg acactagagg gtatagctta
     9541 tgctaataaa aacaaaggta atcatataat aacttcaaaa atagaacacc atgcaatact
     9601 tcatacatgt gaatacttag caaaacatca tggatttgaa ataacttatt tagatgttga
     9661 tagcgaagga aaagtagact taaagcaact agaagattca ataaaagata ctactatact
     9721 tataagtata atgtttgcaa ataatgaaat aggaactata cagcctatta aagaaatatc
     9781 cgaaatagct aaaaaacata agatattatt ccacactgat gcagtacaag ctacaggtaa
     9841 tattccagta gatgttaaag aattaggaat agatttgatg agtatgtctt cacataaaat
     9901 atatggacca aaaggtgtag gagctttata cataagaaaa ggtgtaagac ttcataactt
     9961 tgtccatggt ggagcacaag aaaaaagcaa aagagctggt acagaaaata tacctgcaat
    10021 agttggatat ggaaaagcag cagaattagc taaggaaaat atgcaaaatc atgtagaaac
    10081 tttaactcgt cttagaaata agttaataga tggtgtgtta gaaagaatac cttacacaag
    10141 agtaaatggt agcttagaaa atagattgcc aggaaatgca aactttgcat tccaatttat
    10201 agaaggtgaa ggaatacttt tattattaga tatgttagga attgctggtt caagtggttc
    10261 tgcatgtact tctggttcat tagacccttc acatgtactt cttgcaatag gtttaccaca
    10321 tgaaatagca catggttcat taagacttac agttggagat tttacaacag atgatgatat
    10381 agattatata ctagagaact taccaaaagt aatagaaaga cttagaagca tgtcaccact
    10441 ttatgatgat gcgaagaaac aaggtttagt aaaatagaca taaagataaa gcataaaaaa
    10501 tagacataaa ttgagtagga ggaaaagata atgcaatata gtgataaagt tatggaacat
    10561 tttatgaatc caagaaatat gggagaaatt gataatgcaa gtggtgtagg tgaagttgga
    10621 aaccctacat gtggagatat aatgaagata tttttagaca tagatggaga tgtaataaag
    10681 gatgttaagt ttaaaacatt tggctgtggt tcagctatag caagttcttc tatggcaact
    10741 gaaatgataa aaggaaaaac tataaaagat gcattagaac ttacaaataa agctgttgca
    10801 gaagctttgg atggtttacc accagtaaag atgcattgtt cagttttagc tgaacaagct
    10861 gtaaaagcag ctcttataga ttatgctcaa aagaacaata tccatatacc agaattggat
    10921 ggatatgtta tagatgatgc tcatgaccat gatgttgaag aagaagaata aaaataagtg
    10981 attgtgttat gagaagttta caatgattaa aaagaagtac taatgatata attaggacat
    11041 aaaaaatagc tgtttttaaa atagctattt tttattgcct aaattaagta ttttaacttg
    11101 atttagaggt taattagtta acaaaataat ccataagatt caaataataa agatgattat
    11161 ttttttagaa aatagaacaa gctatatttt aaataagtac aatacttgct ctatatggta
    11221 taataataag taatgcaaaa gatgaggtga aatttgttta tgaataaaaa agtaatgata
    11281 ggtatgagtg gaggagttga tagctctgta gctgcatatc ttttaaaaca acaaggatat
    11341 gatgtcattg gtgttactat gaagttgtgg caagatgatg atgttgttga aattgaaggt
    11401 ggttgttgct ctttatctgc tgttgaagat gctagaaggg tagcaaataa aataggtata
    11461 ccattttatg ttttaaattt tagagaagtg tttaaagaaa aagtaataga ttattttata
    11521 gatgaatatt tggaaggaaa aactcctaat ccctgtatag cttgtaataa acatataaaa
    11581 tttgatgatt tttataagaa ggctagacaa ataggttgtg attatgtagc tacaggtcac
    11641 tatgcaaaga tagaaaagga tgaaagtaca ggaagatatt tgctaaaaaa atctgttaca
    11701 gataaaaaag accaaactta tgcactttat aatcttacac aagagcaact agagcatact
    11761 ttattgccaa taggtgacta tgaaaaagat agagtaagag aaattgctaa ggaaatgggt
    11821 atggcagtac acaataagcc tgatagtcaa gaaatttgtt ttgttaaaga taatgactat
    11881 gcaaattatg taaaaaaaca ttctaaaaag cgtattgaag aaggtttttt tgtagatact
    11941 aaaggcaata tacttggaaa acataagggt atattatatt atactatagg ccaaagaaaa
    12001 gggcttggaa ttacttttgg aaaaccaatg tttgtaatag acataaatcc aataaataat
    12061 accatagttc ttggagacaa tgaagattta tttaaaaagg aacttattgc taaagatgtt
    12121 aactttatat caatagatac gttagaagaa cccttaagag ttcaagccaa aataagatat
    12181 tcagcaaaac catcaccagc tactattcat agagtaggag aagatactat taaaatagtt
    12241 tttgatgaag ctcaaagagc tattactaaa ggtcaatctg tagtaatgta tgatggagat
    12301 attgtagtag gtggaggtat aatcgagaaa agtttataaa aacatctaaa aaattaaaaa
    12361 aataagcaat aaagtatatt aagagtaaat aaagtttata aaataaattt attgtatatg
    12421 aaaatatagt attgattaat tttgtaaaat ttagtaatat aattagtata ttaattaaaa
    12481 aacaaataca gtgataagga atagtaaaat atttgatgtc ttacagagag agggaaatgc
    12541 tggaagccct tagaatatga tatttgaagc agccttttag tagcaatctt gaacaattta
    12601 tcttttataa agaagattta agtagagatt gacggtagtt acgttatagc taaggttttc
    12661 cgaagatatc atactttgta tgataattag ggtggtaccg cgattgtcct cgcccctata
    12721 aatttattta tagggacgag gttttttatt taaacgaaaa aataaaattt attaggagga
    12781 acatgatatg gaaaagatgg gattaaatga aataagaagt aagtttttag aattttttga
    12841 gtcaaaaggt cattatgtag caaatagtta ttctttagta ccaaacaatg ataagagttt
    12901 gttacttata aactcaggta tggcaccttt gaaaaactac ttttcaggtg tagaagtacc
    12961 tccaagtgtg agaatgtgta catctcaaaa atgtataaga acaggagata tagaaaatgt
    13021 aggtataact gcaagacatg ctacattctt tgaaatgatg ggtaatttct catttggaga
    13081 ttactttaaa agagagtcaa taaaatgggg atgggaattt gttacagaat ggctaaatat
    13141 accagaagat aagatatggg tgactgtata tgaagaagat gatgattctt atgatatatg
    13201 ggcaaaagaa atgaatttcc cagaagaaag aatggttaga cttggaaaag atgataattt
    13261 ctgggaaata ggtacaggtc cttgtggtcc ttgttcagaa atatattttg atagaggaga
    13321 agaatatgga tgtgacaatc cagactgtaa accaggctgt gattgtgata ggtatttaga
    13381 attttggaac catgtattta ctcaatttga tagagatgaa gaaggaaatt atagtctatt
    13441 agaaaacaag aatatagata caggtatggg tcttgaaaga atgggatgta ttatgcaagg
    13501 cgtagacact atctttgaag ttgatacaat taaatctata ctagaagcag ttgagaagtt
    13561 aacaggagta aaatatggag agaatccaaa aaatgatata tcaataagaa taataactga
    13621 ccatataaga gctgtaacat tcttagttag tgatggagtg cttccatcaa atgaaggaag
    13681 aggatatgtt cttagaagac tccttagacg tgctgctcgt catggtaagt tattaggagt
    13741 aaaagaatta tttttacaga aactaataga tgaagttata aaagtaaatg ataaagcata
    13801 tccagtttta gttgaaaaag aaagttatat taaaaaagta gttggaatag aagaagaaaa
    13861 attcaatgaa actatagacc aaggaacaga aatattgaac tcttatatag aagtattaaa
    13921 aaatgaagga aagactgtcc taagtggtca agaagctttt aaactatatg atacttatgg
    13981 cttccctata gacttaacta aagaaatatt agaagaagaa catctatctg tagatgaaga
    14041 agcttttaat gaagaaatgg aaaagcaaaa agaaagagct agaaatgcta gaggaaatat
    14101 ggatggagaa agttggaagg aagaccctct atcaaaatta gaatctacag ttgacagtac
    14161 atttaatggg tatagtgaaa tttatggtga aggaactatt gaagctatag ttaaagatga
    14221 tgagctagtt caaagtgcag aagaaggcga taaggtatct atagtactag ataacactac
    14281 tttctatcct gaaggtggag gacaagttgg agattgtgga ttgataacta atgaaaactt
    14341 agttttagaa gtattaaata ctaaaaaagg tgctaataat agtataaaac atataggtat
    14401 aataaaatca ggaagaataa gtaatgggga caaagtgaag acactcgtag atagagaaac
    14461 tagaatgtca gctgcaagaa atcacagtgc tacacattta cttcataagg cattaagaga
    14521 agtattagga gaacatgtta atcaagctgg ttctttagta actccagaaa gacttagatt
    14581 tgatattact cactttgaag cgatatcaaa tgaagaatta aaagtaatag aagaaaaagt
    14641 aaataatgtt attttatcat ctttagacat aaaatgtgat attatgaata taaaggaagc
    14701 taaagaaaaa ggagctacag cactatttgg tgaaaaatat ggtgatgagg taagagtagt
    14761 ttctatggga gattattcta cagaactttg tggaggaact cacttgacaa atacttctca
    14821 agtaggaatg ttcaaaatat tatctgaagg tggagtagca gcaggtgtaa gaagaataga
    14881 agcaataaca ggaaaagctg tttatgaata cctaaaagaa agagatggaa ttatctctga
    14941 agtttgtgta aacttaaaat ctaaagaaga taatttaatt caaagaataa gttctttatt
    15001 agaagaaaat aaaaacttat ctaaagaatt gcatgatatg aaagctaaga tgagtttaca
    15061 atcagttgat tctatttttg attctaaagt tgaagtaaat ggagttaatt tgataactaa
    15121 taaatttgaa ggtatggata tggatacact aagagaaaca gcagataatc taagagataa
    15181 gcttggttct ggtgtagtag tacttgcaaa tgtagtagat gataaagtta actttgtagt
    15241 tactgccact aaagatgtat tagataaggg aatacattca ggtaatattg ttagagaagt
    15301 tgcaaaaata gctggaggaa aaggtggagg aagacctaat atggcacaag caggagctag
    15361 tgatgtatct aaggttgacc aagctttaag ctatgcaagt gaggttatta agacacaagt
    15421 taaataacat tcactcaagg aggtttttag tttggaaaaa gatttagact acactatgaa
    15481 atttgaagga ataccagaag ataggatgag tgtaggagat actattgatt ttgtatataa
    15541 agcattagtt gaaaaaggat ataaccctat aaatcaaatt atagggtatc tcctttctgg
    15601 tgattctagc tatataacta gtcataaaaa tgcaagagct ataattaaaa aatttgaaag
    15661 agatgagata cttgaagaag taatcacgca ctatctgaat agaaagtagg tcaaataaat
    15721 gagaaaaaag attatgtcgt tgtttgcaat agtcattatg atttttgtat caactgctag
    15781 ctatggtttt gctgaagaaa aaggcaaggt catatttatt gatatgaata ggaccaatct
    15841 tggaagtatg atgaatattc caatcttgaa aaatgagata gaaaagagag gctatgtagc
    15901 tctcatgaat ataagagggg atcaaggtac tgatgataga aggtcttatg caagtatggg
    15961 tgcaggaaca agagctagtg taactactga agactatatc aattttcaaa aagtagacca
    16021 agatactgct aaagcttttg aagtcgcaac aggtcaaaaa gctaaagaaa taaatgattt
    16081 aggcattaat agatctataa atgaaaatct tgagtatgga gaatatggag caaatttagg
    16141 gtcattagga aaaactttat cagaaaatgg tttaaagaca tctgtaattg gaaatgctga
    16201 tataattgaa aataatcaat tagttaaaaa cagaaactta tgtttagtag ctatggacca
    16261 atatggaagg attgattcag gtaatattga taatattaat aagaaagatt ctaaaatgcc
    16321 atttggaata tctacagatt atgataaatt actagatgaa actaaaaaat cctataaaaa
    16381 tagtgatgct atatttgtag aacttggaga tacatataga ttagatatgt atagattaaa
    16441 tctaaatgat aatacttatg atataatgaa gaaaaatatt gtaaaaaata taaataaata
    16501 tcttgaacaa gtattttcta tggttggaga aaatgacact gtttatatag caagtgcatt
    16561 tccaagtgac ctagattata agaataagag aagactttct ccaataataa aatttagtgg
    16621 agaagggaaa ggtctactgg aatctgccac aacaagaaag gctggaattg ttgccaattt
    16681 agatgttgga gtagatatac taggtacatt tggtcttgaa aataaagaga tggtaggtag
    16741 aagttatact ttagtaaata aagaggacaa tattagctat ctaaaatcac tttatgaaag
    16801 gatagtgtct atatcttcaa taaggtcaac tattataaat ggctttgtag gcgtaatatc
    16861 tgcttcttgg gtaattgcca tgtttgcgat tttctttaga aattatatgc cacataaaga
    16921 gcgtgtattc aaagtactta aggagtttat aaaattaggc ataataatgc cactatcatt
    16981 tatgttagct cctttgatga attttaaaac tccaatttca atatctttgg gaatatttgc
    17041 aactacgcta atattatact tgttaggtag aaaattattt aaggacaatg atataaagca
    17101 catgggattt tttgcaacac ttactatttt agtcatagtt atagattctg tatttggaac
    17161 atacttaatg caaaataaca taatgagtta tgatgctgtc ataggagcta ggtactatgg
    17221 tgtaggaaat gaatatgaag gtgtaactat agcaagtgca gtatttggct tagcagtatt
    17281 attacattat aagaaggtat ctaaattact tgttgtaatt ttttcattaa taatacttat
    17341 aactagtgca tatccttcaa tgggtgcaaa tgttggtgga gctatatcag aatgtgtagc
    17401 atatttgttg tttataatgc ttatatatga cattaaaata gattttaaga aagctgtatt
    17461 attagctgtt tcagctgtag ttgtcgtttt tgcatttgca gctttagata tactttcagg
    17521 tagtgaatct catttaggta tgtttgtaca gagaattttc ctaaatggac caggagagat
    17581 attacaaaca tttggaagaa aaattcaaat gaatatgcaa ctagcacaga caagtgtatg
    17641 ggtaaatatt ttacttgtag gaattggagt aattgcagtg cttatattta aaccatctag
    17701 acactttaga aagataatga ataattttcc aatcttgttt aaagggttta ttgcatctat
    17761 ggttggttgt ataattactc ttttagttaa tgattctgga atagttgctg catctacagc
    17821 atctatatat atacttatac cactaataat aataagtata aatatgataa tatttgaaaa
    17881 aaataactaa aaaaattttt actgttcata taggatttaa ataaaaaatg caatatgatt
    17941 attaatgtaa actggatgct tacataaaaa atcatattgc aatttttatg attgattatg
    18001 taaaattata tgaagcagta agcctgtgac cttagttatg ataggttcag aatatgtatt
    18061 taatctatag aatataaata gataaaggag gaactatgtt agatggaaga ataatgggtc
    18121 ttgatgttgg tgataaaact attggagttg ctgtgagtga tttgatggga ttaactgcac
    18181 aaggtgtaaa aacaataaaa agagttggca agaaaaaaga tattgaagag ctaaaagcaa
    18241 taataaaaga aaaacaagta aataaaattg tatcaggatt accaaaaaat atgaatggaa
    18301 ctttaggtcc acaaggagaa aaagtaatca agttttgtga attggtaaaa gcagaaacag
    18361 gtattgatgt agagttttgg gatgagaggc tttctacagt agcggcagag aggtctttat
    18421 tagaagcaga tgttagtaga caaaaaagaa aaaaggttat agatatgttg gcagcagtaa
    18481 taattttgca aggatattta gattttaaaa taaattcata aaagaactat aaaattgaat
    18541 acttatgata tataatataa ataataaaaa taaaaataaa tttgaggtga atgacattat
    18601 ggaagaaaat ataataaatt taatagacga aaatggcgtt gaaagccaat ttgaaataat
    18661 attaacatta gaagcagaag gaaaagagta tgcaatatta atgcctttag atgatgaaga
    18721 agctgaagaa gctttaatct ttagaattga tgaggatgaa gaaggtgaaa tcttaatacc
    18781 tttggaaagt gatgaagagt atgaaactgt tgtagctgtg tatactgcta taatggaaga
    18841 agaaggttta aattacgacg aagatgaatc aaatggactt aactagataa gtaattaagt
    18901 ttgtatataa aaatcagata tttattatat ttgctttaat taaaccttat taattgtaaa
    18961 agcatttata gtatatatct gtttttttgt tttttattaa aaataccaaa aaatttatga
    19021 attttggtga ataatatatc ttttatgggt atatatttat attatgagaa taaattaaat
    19081 ttatttaaat gtcttgacaa aaaatgaatt atcaattaca atattattta ataaagatta
    19141 tcaattgaaa atagtaaggg tgatgttatg gcaaatacaa tggatttatt aaaagacaag
    19201 ttaaaagaaa ctggttttaa gattacgcca caaagaagag caatagttga aatattgctt
    19261 aaacatgacc attcacatct aagtagtgag gaaatctatg atttagttag ggttgactgt
    19321 cctgaaatag gcttagctac tgtatataga acaatgcagc tattggatga aataggatta
    19381 atatcaaaac taaacttaga cgatggatgt ataagatatg agattagtct acataaagaa
    19441 gactgtcata atcatcatca tcttatttgt aaaaattgtg gaaagataat ggaagctaaa
    19501 gaagaccttt tagacaatat agaaaaagag atacaatcat tgtacaaatt taaaatactt
    19561 gatcatgatg ttaaatttta tggactatgt gacgagtgta atggagtaag cgattcagaa
    19621 gaataaactt ctattgctta atgtcgatgg aagaatagta tagaactttt atattctatg
    19681 ctattcttcc attttttatt ataaaccatc tataaaatgc aaatatacag ttttcaagat
    19741 gcttatagtt ttaatattga aaatataatt aatcctataa gtacaaaaat gaatgatttt
    19801 tctatgaaat tactaaatgg tcctccaaca gttaaaaaac ctaaagaaat tttcttttcg
    19861 cttagcggat aaaaaattgg tattccttgc tttgtaaata aatctcctaa aaacatatga
    19921 cttgcataac caatcgtacc ataaaaagat aaattattaa tattagtgat taattctatc
    19981 tgttttagta aaaaatatac tattatagtt gcaaatatgc tatgagaaaa acttctgtgc
    20041 tttaaccagg gaaaaattga caacattaga caaaaaattg taaaatatat ttcaacttct
    20101 atgatgtata agcatatagc taatagtata aatattaatg atagaaaaac ttttcttaga
    20161 aatgtgtggg ttagttttgc ttcaataatt ataattgcaa caaatgtaat tattgcgctt
    20221 aaataaaaat tatcatttat atttattgaa ataaagaaaa taataatatt aacagcatat
    20281 ataaaaattc tatatatgta tttggataca ttgtttttta gaatgaaatt tgagaccata
    20341 gaattagatt tatccaaatc gggtagtaca gcaaaagtag ctgaaactaa aatatcaact
    20401 aaagatatag gaattttaaa tattaaagat gtttggatag ctgttaagat tcctattgca
    20461 caatgagttt taccacgcat aaatcctcct aaattatccg atattaaatc atataatatt
    20521 tagaggatat caaaataaaa taggtttgtc aaaataatac gttaaatgta ttttaaatat
    20581 agcttaaaat atgatacgaa ttatggtaca ataagaataa ctggtagcga gtgcgtgatt
    20641 tgatattatt tatgattgta gaacgtgtta aaaaattaaa tagacagaag gagatgatgc
    20701 aattgttcaa aaagaacacc aacaagataa aggtaatggc cttgggtgga cttaacgaag
    20761 ttggaaaaaa tatgactgtt gttgagtata aagatgaaat aattgttata gatgctggac
    20821 ttagttttcc agaggatgaa atgttaggag tagatatagt tataccagat ataacttatt
    20881 tagtaaaaaa tagggataaa ataaagggta tatttattac acatggacat gaagaccata
    20941 taggagctct tccatatata ttaaagaaaa taaatgtacc tgtatatggg gcaagactta
    21001 gtataggtct tatacaagta aaacttaaag aacataaaat gaataatgtt aagttaaatg
    21061 ttataggtcc tagacaagtt ataaagcttg ataatatgga agtagagttt ttgaaaaata
    21121 accatagtat accagatgct tactctattg caatacacac agaccaaggt ataatatacc
    21181 acacaggaga ttttaagatt gacttgactc ctattgatgg agatgttatg gatatgcata
    21241 gaatctgtga gttgagcaaa aaaggagttc ttttaatgct ggcagatagt actaatgcag
    21301 aaaaaccagg atttactatg tctgaaaaga ctgttggggt aggtcttgac gaattatttg
    21361 caaaaggaaa tggcagaaga attatagttg ctacctttgc atccaacata cacagattac
    21421 aacaaataat aaatactgct gaaaagttta atagaaaagt tgctatatca ggtcgctcaa
    21481 tggtcaatgt agttggagtt gcaaaagaat taggatacct agatatttct gatgacatgc
    21541 ttatagattt aaatgatata tgtaagtatg aagatagtga attagtgata ataacaactg
    21601 gttcacaagg tgaacctatg tcagctcttg ctagaatggc tttttcagag cacaaaaaag
    21661 tagaaataaa gagtggcgat ttagttataa tatctgctca tccaatacca ggaaatgaaa
    21721 aattaatatc aagagtaatc aatttcttat ttgaaaaagg agcagaagta gtatacagtg
    21781 atatagctga tatacacgtt tctggacatg cctgtcaaga agaattaaaa cttatacatg
    21841 cattagttag accaaagttt tttatgccag ctcatggtga atatagaatg ttaaaaagac
    21901 atgctgaaat agctgaacag cttggtatgg ataaagaaaa tatttttgtc atgcaaacag
    21961 gagatgtttt agaattagat aaaaattctg ctaaggtagc aaaccgtata caaacaggaa
    22021 acatattagt tgatggtcta ggagtaggcg atgtaggtaa tatagtttta agggatagaa
    22081 agcatttatc agaagatggt cttatgatag ttgtagttac tatttccaag gatgagggta
    22141 aggtcttagc aggtccagat ataatttcca gaggattcgt atatgtaaga gagtcagaag
    22201 atttaatgga tggagcaaag gatataatta agaatgttct aaatgaatgt gaagagaaaa
    22261 atattaaaga atgggcttat ttaaagaata atattaaaga aaacctaaaa gaatatttat
    22321 atcaaaaaac taaacgaaat ccaatgatac ttcctatcat aatggaagta taatataaaa
    22381 aaactataaa tttgaaaatt agacctcttg taatttttaa agtaatatca aaaatctaat
    22441 tgatattatc ttaagaatta caagaggtct ttttaatact cattttaaaa aatatatata
    22501 ttacataaaa taccaattat aacaatcttt tattagtaaa aactacttat atataaattt
    22561 tactgatagg aggattctta tggatgatgt ttcaagacaa aatgctataa aagctttaaa
    22621 acaaacaaaa atggaaatag caggagagta tggaatgaat tatgaagatg cttttgaaat
    22681 aatagaaaat gcgtctaata aaggtgtttt agaaggttac tttaaaaagc ttgaaaagaa
    22741 aaaaaattta ggtcaaggta tatctaggca tttagaataa aaaaagttaa atgtgagaaa
    22801 ataagtttaa ataaacttta agaggtgaca tcgatgaaaa aattagctag tactgctttg
    22861 gctatattaa ttgcgcttac tcctttaagc ttttcttttg caaataataa ggaaaatgct
    22921 gatgccaacc aactaaatat atcttcaaaa tcagcaattt tgatggatgt tggtagtgga
    22981 caaattttat atgaaaagga tgctcataaa aagcttcctc ctgctagtgt aactaaggta
    23041 atgactatgc tacttatagt agaagctttg gattcaggca agattaagct tgatgatgaa
    23101 gttcaagtaa gtgaaactgc atctagtatg ggtggtagtc aaatattcct agagccagga
    23161 gaaactcaaa aggttgatac attattaaaa ggtatagctg ttgcttctgc aaatgatgct
    23221 tgtgttgcta tggctgaaca cttagctgga agtgttgaag gatttgttga tagaatgaat
    23281 gctaaagcaa aagagttaaa tatgaatgat actcattttg caaatacaaa tggacttcca
    23341 gttgcaaacc actatacatc tgctcatgat atagctttaa tgtctagaga actattaaaa
    23401 cacgaaatga taagtaaata cttaactaca tggatggata aggttgtagt agggaagaaa
    23461 caagttacag taggtcttgc aaatactaat aaacttataa agcattatca aggagcaact
    23521 ggtgttaaaa ctggttttac tcaagaagca aaatactgtt tatcagcatc tgcaaagaga
    23581 ggtaatactc atctagttgc tgcaacttta ggagcagaga cttctccaga aagatttaat
    23641 gatgcctcta gccttttaaa ctatggattt gctaattatg aaagtgttaa gttatgtagt
    23701 aaaggtgata atatagcaac acttacattg gataaggctg atgaaaacaa agttaaattg
    23761 gttgctaaag aagatttaaa tgctcttatt aaaaaaggaa gttctaaaga atttgagaag
    23821 aaaattgaaa tagtaaaaaa tcctaagatg ccaattaaaa agggaactgt tttaggaaaa
    23881 ataaagatat gtaaagataa aaaagtaata ggcgaagtgg aattgataaa tactaaggat
    23941 ataaataagg caagctatct acaaatgctt caaagaataa ttgataatat gatataaata
    24001 attatcagat gaaatataat tataatacac aaagatatga ttataataca cataaaaaat
    24061 acccttatgt aattatttac cataggggta tttttattta ccatatagaa gtattttata
    24121 atcttatgat ttttatagaa atatagaaaa gtttatgtat cttaaaatat catttgattt
    24181 aatatcatat atagggttgt ttaacaatta tctaagtaaa ggcatactac cagtaagttt
    24241 attcactttc tttttattcc cataaattgc tatcccaaaa tactgtaact catcttctgg
    24301 aacactagca atcttttgcg taaattcttc ataaagattg caactttgtg ctacatctga
    24361 aaaatctaca acagttaaat cgttaaattc caaagtatat aattttttac gtaaatcttt
    24421 tattatttct ttgtttcctt ttaaaatagg tacaggaatg tcaataattc ctaagtggct
    24481 attacctgtt ttgtcaatta catctggccc aacagtttct ggggcatgtt ttcctagtgt
    24541 aattcccatt atagctgctg tattggatat aattcccatt ggtaaatttt cgtctattac
    24601 catgacacat tttgaatttt tattttccat ttttatttct cctttttacc ttatttaaag
    24661 ttgtttgaat catcatttat aaatatattt tgatattgct ttggtgtaag accaataaaa
    24721 ttcttaaaaa agtttgtaaa gtgactttgg tctacaaatc ctgtttgtat cgcagcatct
    24781 ataggttcta cgcctttttc taaaagtttt ttagctttgt ttacccgtat attttctagg
    24841 tatctatatg gtgtaatacc ttttaatttc gtaaaattac gaagtagaga gtacttattc
    24901 atacctgcta tagtgcttaa ttcgtcaagt actatatgtt cagcataatt attttctaga
    24961 taatcacata ctgcttgaat ttctatattt gtattttcca agttagattg caaatttggc
    25021 tctgtatgtt cttcaattaa ttgttcaatt aggaagaaaa aaagctcttc ctttttaaaa
    25081 tccaattctt cttccatgat tatatagtgt aattcttgta gtagaggtac tagctcactg
    25141 cgaaatataa caggttgatt aaattttggt aaataatttt tacctgtaat ttcaaagaca
    25201 gtttttttca ttatttctgg cttaatatta atacatctgt aatcaagtac tttatcatct
    25261 atttgttcac aagtatgact atcaaaaggg ttaaaaagta gcaaatctcc agtacttgtg
    25321 gtatactctt tatttttaca agataaatat ctttgacctt tttctataaa accaattaca
    25381 taatgttcat gaaaatggtt tgggaatttt tgcattatgc ctttaaatgt atatgcttca
    25441 atttttaatt ccctatcaaa gcatactgtg cgttcttctt gtctcactta ggtaacctcc
    25501 tttcatgtta tacaaagtgt aacacatatt tttttatact tcttgtataa tattgactaa
    25561 gagacaataa tgtcaattca tgtcaatata atattacact tacagtaggt gtaattatta
    25621 tattttcaca atgaatatga tataataata aaagttataa aataaaaagc aaattttaat
    25681 tttatgccta gaagttaaaa ttattataat gtaaaataat tttgattata agctatttaa
    25741 ttgttataat aatattaaaa atttaattta ggagatgtac attaatgtta agtatgaata
    25801 taggagaaat agtagcaagt ttagttggta tagctatggc aatatcaata catgagtttg
    25861 gccatgctta ttcagctcac ttattgggag atgatacagc taaagcttat ggtagaatga
    25921 cattaaatcc tgtaagacat gttgacccaa taggtctttt ggctatgttt atccttaaga
    25981 tagggtgggc aaagccagta ccagtaaatc caaataactt taaaaactat aagataggaa
    26041 acctgatagt ttcacttgca ggcgtttttt gtaatgtttt gactgctata ctgtgtgtaa
    26101 ttatcaataa gtatgtgcat atgtacgcta taaatacaat agctggatat gtgcttttat
    26161 atagtatggg atttgcagct tttaacttac ttccgatacc tccacttgat ggatggggag
    26221 ttatatctac atttgtacca tataaatgga atgaatattt gtataaattt gaaagtatga
    26281 gttatataat tttattaatt gctttattta ctggtatata tcaaataata ctatcaccaa
    26341 tatatacagt gttgctaaaa atagtatatt tatttgttta atataataaa cagattaaag
    26401 agggattgat atgaagtata atatacaatt acaagtctat gagggaccat tagatttact
    26461 ttatgattta ataacaaagc ataaaataga tataaaggat atatctataa tagatataac
    26521 taagcagtat ctaaattatt tgaaaatgct agataaaatg gatttagaga taactagtga
    26581 atttataact atggcatcaa aacttttaga gataaagtct aaatatctgc tttataagca
    26641 aaaagatgaa gaagaagacc caagaataga acttatggaa aagttagaag aatatagaaa
    26701 gtttaaggta gcatcacaag atataaaaga aaatataact tatgttaatg agagatttta
    26761 tagaaataaa gaagagataa ttattgatga caatgtggat ttagaagata tatcaattga
    26821 agcaataaaa aatatacttc catacatatt taaagttaaa acatcacaga tagaaaatgc
    26881 taatgatgag aaactagata aaatagtaag gaagaaaata atatctgtag aagaaaaaat
    26941 attatatata agagatatta taaaagagaa gattgaagtt acttttacaa atattataaa
    27001 atcatatgaa aatgatgaaa taatagctac ttttttatct atattagagc ttataaaaga
    27061 aaaagaaata gtcgtagtac aagatatatt ttttgatgat attctaataa gaaaaagttc
    27121 ggagtgttaa aatgaaaaga gaagatataa agtatattat agaatctgtt atgtttgcat
    27181 atggggaacc aataagtata aaagaattaa actatataat aaataaagaa ctttcatcaa
    27241 aagaaataga gattatgtta aatttactta ttgaggagta cagagaacaa aatagaggta
    27301 ttcaaataat caagttagag aataagtacc aaatgtgtac aaataaagac tatgcagaat
    27361 atataaagaa gattattgaa ccaaagaaaa agaaaagttt gagccaagct acattagaaa
    27421 ctcttactat aattgcctac aaacaaccaa taacaaaggt agaaatagag gatataagag
    27481 gagtaaaatg tgataaggta ttacaaactt tgtttgaaaa tgaacttata agagaagctg
    27541 gtagattgaa taagataggg aaaccaataa tttataaaac aacagatgaa tttttaaagt
    27601 tgttgaatat agaaagtctt gaagaattgc cacctataga aaattatcaa gaagtggtaa
    27661 caaatgaata aaaatataaa atcacaataa tttagtatgt aattttaaat tttaaaagat
    27721 ttaaaatgct ctaagttgtt gtgttttttt gtataaatat aaaattataa ttctcttaaa
    27781 ataaatatgg ataattaagt ataaatagta attttttgat gataatataa acatggagct
    27841 taatgaatta tttaatttta agtatttaat aatagctgta atttttatta taagtgtaac
    27901 tatgttgatt ttaatatctc atataaacat cttaataata gctgatataa aaaacaaaga
    27961 tatttgttta aagcttaata taaaatacat gtttaattta ataaatataa atagacaact
    28021 ttatccagct gaaaatagta aaaataatga taaaaaagag gggatgaaga ataatataga
    28081 ttcaagtata ttattagctg atgacctttt gagcatttat agattattaa aaaagataaa
    28141 aatacatgaa ctttattcaa atattaattt tggaactgga aatattggct taacatcttc
    28201 tgtatatgta ttgataaata ctttgtatgg aaatttattt aatatgattg atgcagaaaa
    28261 aatgtattta aatgtaaatc cagattttac aaaagattat gttttaggaa atataaggat
    28321 acatataagg ccaagaataa aagctttatt taatattata ataatgatta ataaaattat
    28381 gaataaaaat aaaggaaata aggaaggtga tagtaatgag agcaacaggt tcgatacaga
    28441 gtcttatgga aacaactctt gaaacaatta aaggttctat agatgcaaat actattatag
    28501 gagaccctat aaaaacagat actacagtgg ttgtacctat atcaaaggtt acaattggct
    28561 ttggtatagg aggaggagaa tactcaaaag gatatgatga taaagatagg gaaattgaat
    28621 taaaaaatga aaaatcagat acgaattttg ctggaggtag cgcaggggct atatctgtac
    28681 aaccagtagc atttgtagta gttgaaagtg gagagactag aataatgagt ttagatagta
    28741 atatcaatct agtggataat atattatcta taactcctag agtattggag aaaattcaaa
    28801 atatatcaca aagtaataag aatcaagata aaaacaatat gtaagaataa aatattttta
    28861 aatatgtata tagttttaag taaagtagtg gggttaatat aaatcccact attttgttgt
    28921 gtactttaaa catttatatc taataagatt ttagtttaaa acaatttaaa tagataataa
    28981 agttgatata tagtataatt ataaaaagaa aagaagtatt atattataga tatataaacg
    29041 catttgagat ataagaggga gttataaatc attagttttg aggtgaatgt tatgagcgat
    29101 aaagaattat ctattttgtt gttagaaagt atgaataatt ttcataattt aataaagatt
    29161 attaataacg aaagatataa aaaagataag gttcttacag aaagacagtt ctttgcctta
    29221 gtaaaaataa gaaaacatga taaaatagag cttaaaaatc taagtagaga tttgcatgta
    29281 tctacttcta gtctgtgtat actacttaat aaattggttg agcaagagta tgtatatagg
    29341 gaagaagata gtagagatag acgaaatact ttttatggaa taactaaaaa tggagaaaag
    29401 atattggata atgaaatact aaagtttgta tctataataa gtgataaaat ggattgccta
    29461 gatatagata ataaagataa attatttact tctttggaag agtctaaaaa tataatagaa
    29521 caattatttt aaaaaggaga atttattatg aatataaagt attctaaaga gatattaaaa
    29581 agtttagaaa atgctataac agtagtagct ctatttgagg gagaagatgt ttttacgaca
    29641 tttgataaaa taaatatgat aagcagaaat tttaattcta aagaaaatag tatagatact
    29701 gtaacattgg ttgaaaataa caatttggta aatgtttgtc ttgttggttt tggtaagaga
    29761 gagttattga atagagaaaa aattagaatc ataggtggga atttgtgtaa aaaattaaaa
    29821 aatatattga aaaatgaagc atataagagt agtgatgtta atgtactgaa tcttaattta
    29881 aatagtgatg aaataggttg ttttgtagaa gggatattac ttggaaatta taagtttgat
    29941 aagtataaga caaaaagtaa ggattttgaa agaaaagaag ttgatactat aactatattt
    30001 actgaaaaag aagttgagtg tcaaatagaa aaggctaaaa tattagctaa ttcaactata
    30061 atagctagaa atctagttaa tgaaccatca aacgtaatat atccaaaaac attagcagta
    30121 gaaacagtta aacttggtgc agagtttggg tttaatgttg atgtttatga agaagaaaaa
    30181 ataaaggcat tgggaatgga tgcttttttc gctgtttcaa gaggttcaat taataaacca
    30241 agattcatag taatgagata ctttggagat aaagaaagta cagatatatt aggtctagta
    30301 ggtaaaggat tgacttatga tactggtgga tattctttaa aatcaaatgc ttctatgcta
    30361 gatatgaaga cagatatggc tggtgctgcg agtgttatag gtgctatgtg tgctatatct
    30421 caaagtaaat taaagaaaaa tgttatagca gtagtggcag catgtgagaa tgctttatca
    30481 ggaggttcat acaaacctgg agatataatt agttctatgg caaagaaaac tattgaagtt
    30541 ttaaatacag atgcagaagg tagacttaca ttagctgatg caatatacta cattataaac
    30601 aatgaaaaag taacaaaggt ggttgatgta gctacattaa ctggtgctgc tttaacattg
    30661 ttaggcaata ttgcaactcc tatagtaaca aataatgatg atttttattg tgaattagaa
    30721 aaagctgcca ctttatcagg ggaaagggta tggaagatgc caatttatga tgaatttaaa
    30781 gatatgataa aaggagaaga agcagacctt aaaaacactg gtggtaaaaa tgctggatgt
    30841 ataacagcag gagcttttat tggtgaattt gttgggaata ctccttggat acatatggat
    30901 atagcgggga caagtacatc atcaaaatca attggataca aagcaaaggg ggctacagga
    30961 gaacctgtta gaacattgta ctatttggca gaaatctaaa aaacaattat aaaatataat
    31021 ttataaagcc acatggatat ttttgtaatt taagattatc tatgtggctt tattttttat
    31081 ttaaataaat tatttactct ttttcttttt ttcaagtata aaagtatctc tattaaataa
    31141 aagagcaggt atgtacataa tactagtaac aaaaataaac gtaaaaatag cagttgtgct
    31201 atgacttaaa aaaccataat agtacctaga gttggtaaat gtgtttagta aaaatatgaa
    31261 tgaaaaataa ataaaacata tagctaagtt atctctaagt gcatatctgt taactctaaa
    31321 ttttctaaca tcaaccatat atatatctgc ataaattatc aagcacttag ataagataaa
    31381 gataaaatat attaagctag gaaattcata gcccaagttt ttagaaaaaa tgagttctcc
    31441 aactgaacaa actaaactcc aagaaaaaaa tatattaaaa agatgatact gcttaaaaat
    31501 tagtatggct atacacagat atatacaaat ataattgatt ccaattggta aagagtttag
    31561 agtactataa ttacctgaag aaataagtaa taattgctct agagccataa ttattacaag
    31621 taatgtacaa attatcttct caactgtata gctataaggt aaaatatttt tggttagttt
    31681 tggacaatag tataaaaata atccaaatat aaagagactt acaaattgtt cactagaaaa
    31741 aagaaaaatt ttaaacatat aacattctct cctataataa atttaatatg tatctatata
    31801 aatatattca ttatatctca atttaatata taaaaaagat aataatagtt agatttttta
    31861 tatgaatttt aatagg
//
LOCUS       DAGSWI010000037        31665 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.037, whole genome shotgun sequence.
ACCESSION   DAGSWI010000037 DAGSWI010000000
VERSION     DAGSWI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31665
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.037"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            5..346
                     /locus_tag="MJF80_001531"
     CDS             5..346
                     /locus_tag="MJF80_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HBZ0280201.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNFKECESYLEGKNELYE
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEVLEEELTK
                     INSHLELEDLR"
     gene            489..887
                     /locus_tag="MJF80_001532"
     CDS             489..887
                     /locus_tag="MJF80_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280202.1"
                     /translation="MQKDVWLYSWDDECFGSDEYESKEEAIQGAKEELRRFGEVSRLV
                     YVGQKEEVNIPNLNVEDALEHVQDRIDNEFMGYGEDWFEKIRVEDILILENRISKVFK
                     KWIDEFGYKPYWFVVRDTEEIELNEVANES"
     gene            935..1660
                     /locus_tag="MJF80_001533"
     CDS             935..1660
                     /locus_tag="MJF80_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HBZ0280203.1"
                     /translation="MNEIMTNENLRVVADDLVTVYETDTGEKIVFARELHNNLEVKRQ
                     FIDWIEDRIKQYGFKENEDYSVFHKNMKNSTGGRPSKEYVLKLDVAKELAMVQNNNKG
                     REIRRYFIRLEKLLNRTLSNSQLSQINTIVNESLLKMEVKHNIQIEQLKKECSEYYRP
                     TSKTKYDISSYIKERLGISKVNEEFELVKKRTLLVLGADKWEDIPKDVLLNSLNLIDE
                     SIRIIKSERKTNQISFFEKDNFC"
     gene            1754..2242
                     /locus_tag="MJF80_001534"
     CDS             1754..2242
                     /locus_tag="MJF80_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280204.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSSVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKIMDMLYPRIKENELPLF
                     KN"
     gene            2988..3161
                     /locus_tag="MJF80_001535"
     CDS             2988..3161
                     /locus_tag="MJF80_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280205.1"
                     /translation="MATKSILKNVDVRKKAFGRNLVSALENAKNKQEKEVVLSKKCSE
                     VPKDKIKDIFGRF"
     gene            3161..3772
                     /locus_tag="MJF80_001536"
     CDS             3161..3772
                     /locus_tag="MJF80_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBZ0280206.1"
                     /translation="MSGYLIVNLSNMLGELEEEEVKKILSSFSCPLNKDVEEFLKNKA
                     IEFSKQGLASTHLVLTSYKGKPVIVGYFTLANKYFTIKRKTLSNSLARKIVKFGQYNE
                     ELRRYIIGAPLIGQIGKNYSNNYNKLIKGDELLKIACDKIKAVQLDMGGKIVYLECED
                     KPKLIEFYKDNGFVDFGKRSLDKDETDSLDGDYLVQMLKYLKK"
     gene            3849..4037
                     /locus_tag="MJF80_001537"
     CDS             3849..4037
                     /locus_tag="MJF80_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280207.1"
                     /translation="MEIKKNTQDVVSERKNSLDSEFKIPASGVCYMAEFIKESREIIK
                     ELDKHFESCLDVLSKARL"
     gene            complement(4017..4334)
                     /locus_tag="MJF80_001538"
     CDS             complement(4017..4334)
                     /locus_tag="MJF80_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280208.1"
                     /translation="MSKNIDIRNLKQFQKKIERMEQNLKPDFDKIRTIILSKKFMNQH
                     TNFDSFDELLAFGNYIVNSEEDFLAIPDNEFDLFIVKNTDFPDWQTMLDSAYSKYLES
                     CLR"
     gene            4458..4685
                     /locus_tag="MJF80_001539"
     CDS             4458..4685
                     /locus_tag="MJF80_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280209.1"
                     /translation="MGFEISGNLNFDSVIDDLKKEVENNPTIFTSQNVGNKFKEKCKI
                     CEKISEFEILEDGKVKCLECGTEFELNLKVE"
     gene            4755..5447
                     /locus_tag="MJF80_001540"
     CDS             4755..5447
                     /locus_tag="MJF80_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0280210.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            5425..6678
                     /locus_tag="MJF80_001541"
     CDS             5425..6678
                     /locus_tag="MJF80_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBZ0280211.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANAIL
                     RIGFKDGELYICNEIYVHEMDTSEIITIANSKGLEKSLFMYCDSAEPDRIKMWKSAGY
                     KAKGVKKGPGSIKAQIDYLKQLRIHVHPSCINTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            6684..8135
                     /locus_tag="MJF80_001542"
     CDS             6684..8135
                     /locus_tag="MJF80_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBZ0280212.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFVERGNSFVQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEIQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDE
                     LERLKKQKEESIKEYEDAFPTKKKTEEGEPLDE"
     gene            8128..9168
                     /locus_tag="MJF80_001543"
     CDS             8128..9168
                     /locus_tag="MJF80_001543"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBZ0280213.1"
                     /translation="MNNIDYWIKTFNQLEEDVHNKGDNFNKELEKQYDIVLYNILREI
                     NNWYIKFAKDNKISMQEAEKLLNLRELAELKLSLEEYIKYGEENVISQKWMKELESAV
                     KRVRISRLKALELKIRHQVEVLYSKEYEDVNTLIADTYQDSYYHTAFETQKGIGIGLM
                     IAMLSMNNINKIVSSPWTTDGITFGNRIWNKHRPRLMSELNKGLKETLINGISPENLT
                     NKINKNFNTSKEEAKLLVKSELAFFSSLSQRDCFNSLGVEKYEIVSALDSKVCEKRCS
                     PLDGKVIEMKYYEIGVTAPPFHPRCRCVIVPYFDGEESYRAAREEDGGTYYVPSSIKY
                     KEWHKKYVKNTY"
     gene            9225..9467
                     /locus_tag="MJF80_001544"
     CDS             9225..9467
                     /locus_tag="MJF80_001544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280214.1"
                     /translation="MLKLYILSIIVFCTGFYLYMLRLGNVKEVIEALKKYKAWKNYKL
                     IFIALFPLLNFIIGLIFIVVSLFATNKDIIESLEDE"
     gene            9468..10034
                     /locus_tag="MJF80_001545"
     CDS             9468..10034
                     /locus_tag="MJF80_001545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280215.1"
                     /translation="MARFVKKAVEVEAFKFDIDIEPPIWIRKEIERGNAWVEHSIDKE
                     HTKMFIRTIEGLIYQVNIGDYIIQGVKGEIYPCKADIFEMTYEKVEYTATIENLTNYA
                     ENLEPGHKYIDKGSSKKNSLEFSVKLKLDTKDFDKNIENATKEIETFNEAVGRLEKKI
                     NRISAKENKVDIDKIVKQLEESLRERIE"
     gene            10053..10325
                     /locus_tag="MJF80_001546"
     CDS             10053..10325
                     /locus_tag="MJF80_001546"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280216.1"
                     /translation="MFKQKYIKKSNKISARNIIAFIITVIGITLGIFIGINIIMAHVL
                     GIANMVDNNTFTCVRLVYGLVGVISGYLIGKAIYLIASLISYIIYE"
     gene            10375..10605
                     /locus_tag="MJF80_001547"
     CDS             10375..10605
                     /locus_tag="MJF80_001547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280217.1"
                     /translation="MKEYIIWFKSGNSISGIVDEDVADKLMQDFIEADSDCRNLKGYL
                     DEDGTTIIDLSQIEAISINNCSENNNIGFNPR"
     gene            10646..11209
                     /locus_tag="MJF80_001548"
     CDS             10646..11209
                     /locus_tag="MJF80_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HBZ0280218.1"
                     /translation="MDWLKELLEGIKIEDNKIDVASLQKSIEKKIKETTVTQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSVKCRDEIALKAHLDMEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            11221..12120
                     /locus_tag="MJF80_001549"
     CDS             11221..12120
                     /locus_tag="MJF80_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBZ0280219.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWESKTLTNQRKWSTLVHPADIDQTNYVASI
                     GNITQVYNEEQKFPEMDAYCISKIYADWIALGKTAETTALTTANILEVFDKLMEKMTE
                     ARVPAVGRILYVTPGVDTLIKNAKEIQRTVNIKDGGTSLNRQTTDIDSVKIVKVPSNL
                     MKTVYDFTTGWKAGAGAKQIFMSLIHPSAIITPVSYQFSTLDEPRAVTEGKYLYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            12120..12350
                     /locus_tag="MJF80_001550"
                     /pseudo
     CDS             12120..12350
                     /locus_tag="MJF80_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12423..12824
                     /locus_tag="MJF80_001551"
     CDS             12423..12824
                     /locus_tag="MJF80_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280220.1"
                     /translation="MENNLIDDIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTTYGKNKILTFRCLRW"
     gene            12818..13165
                     /locus_tag="MJF80_001552"
     CDS             12818..13165
                     /locus_tag="MJF80_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280221.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDAITKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            13165..13455
                     /locus_tag="MJF80_001553"
     CDS             13165..13455
                     /locus_tag="MJF80_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBZ0280222.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            13565..14371
                     /locus_tag="MJF80_001554"
     CDS             13565..14371
                     /locus_tag="MJF80_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280223.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNKLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            14720..15157
                     /locus_tag="MJF80_001555"
     CDS             14720..15157
                     /locus_tag="MJF80_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280224.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPHSEDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            15150..15326
                     /locus_tag="MJF80_001556"
     CDS             15150..15326
                     /locus_tag="MJF80_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280225.1"
                     /translation="MSKTLSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENDLYSFS
                     EVEDRINKFMKGVS"
     gene            15327..16637
                     /locus_tag="MJF80_001557"
     CDS             15327..16637
                     /locus_tag="MJF80_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HBZ0280226.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDIGLVESSLIYWAAGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            16658..17134
                     /locus_tag="MJF80_001558"
     CDS             16658..17134
                     /locus_tag="MJF80_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBZ0280227.1"
                     /translation="MSKNITMNVRDAISGSKAECFVTIKGKRYNFMQAINLEAKMEKN
                     KSEMPILGSITKGNKSTGSKFSGSATFYYNTSIFRELLYEYKETGEDIYFDMQITNED
                     PTSSVGRQTIILEDCNLDSGIIAKFDADGEYLDEDVDFTFENWRIVEKFNIANGME"
     gene            17187..17444
                     /locus_tag="MJF80_001559"
     CDS             17187..17444
                     /locus_tag="MJF80_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280228.1"
                     /translation="MIKIKDKYEIKDSISFDYSKKRTIEECVSEIYKKAGKYLIDIAD
                     KLALDTIEGSSLKPITIKIKLDEDGIATIEKETKYLIMEVE"
     gene            17446..17886
                     /locus_tag="MJF80_001560"
     CDS             17446..17886
                     /locus_tag="MJF80_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBZ0280229.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFVGEDGKPVEWELKAIDSD
                     RDRQLRKNSAIRVPVIGKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            18209..18367
                     /locus_tag="MJF80_001561"
     CDS             18209..18367
                     /locus_tag="MJF80_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280230.1"
                     /translation="MDNFLQNVLERLSVSLIVFLVSNLLKKRKKPLKAGTKSGWEFDL
                     KIRFHKFK"
     repeat_region   19144..19432
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19145..19173
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            19592..19762
                     /locus_tag="MJF80_001562"
     CDS             19592..19762
                     /locus_tag="MJF80_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006862428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBZ0280231.1"
                     /translation="MSPKKIGRPVVGSPKTNDIKVRVDDETNEKLDEYCKKNNLTKAE
                     AIRQGIHLLLEK"
     gene            19888..20694
                     /locus_tag="MJF80_001563"
     CDS             19888..20694
                     /locus_tag="MJF80_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HBZ0280232.1"
                     /translation="MMNDLQIFKSEDFGEIRTVDIDNEIWFVGKDIAEALGYSNTREA
                     LKTHIDNDDIAEVVIHDGSQNRNMKITNESGLYSLIFGSKLETAKNFKNWVTKEVLPA
                     IRQHGAYITNNADPQALREKANEIESLDTVNKTIEILTPFLDNAGIDEKAKLLTAKTI
                     YKKAGIELPLEIEEKEHFFDTKQIATKLNMYSKSNKPAFHAIGEIIKKLDIQDNEKLV
                     VLESKRGWSGSVNKYSQSVIDKIRHWIGENNKPAKIQGEKKNFHVVYKIE"
     gene            20751..20927
                     /locus_tag="MJF80_001564"
     CDS             20751..20927
                     /locus_tag="MJF80_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280233.1"
                     /translation="MIEDLLNEYNLKTDEDVEYFVKFANALYELKQNSEEKFQEYAEI
                     LRGILREQEERKNK"
     gene            21348..21542
                     /locus_tag="MJF80_001565"
     CDS             21348..21542
                     /locus_tag="MJF80_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280234.1"
                     /translation="MWCVKELVEYKNLKSEFSIILTKWYVNSNIKDFTSLPSIGFILT
                     MWYVKKELLRRMLLAGEILY"
     gene            21482..21739
                     /locus_tag="MJF80_001566"
     CDS             21482..21739
                     /locus_tag="MJF80_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HBZ0280235.1"
                     /translation="MVCKEGTFKKNVTCRRNFILTKWYVKSTSASLLMLFVLCFILTK
                     WYVKSFIMFNLAFVLVSFILTKWYVKNGAIVDSIDTNIILY"
     gene            21946..22728
                     /locus_tag="MJF80_001567"
     CDS             21946..22728
                     /locus_tag="MJF80_001567"
                     /inference="COORDINATES: protein motif:HMM:NF021378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBZ0280236.1"
                     /translation="MGLFGGKEPCSICGGKGKNKVLENEYLCNNCFINFTIFSRERLK
                     ATSAMQVLADHEGIRKFINFSKTNRELLAKFVETNRINKFVSIDEDNNFIKISDIRKG
                     GDVIENVYAIDEIMQFELLEDGESIVSGGLGRAVVGGALFGATGAIVGGITGKKTTRK
                     VVDTFKIKITFNNINNPLEYVNLINTKTKTNSSIYQNACNEAQEILSVLSIIIKNNDK
                     TDNTQSKTSDSIEQVKGLKELLDLGAITEDEFNTKKKELLNL"
     gene            22795..25152
                     /locus_tag="MJF80_001568"
     CDS             22795..25152
                     /locus_tag="MJF80_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBZ0280237.1"
                     /translation="MATIQTSIRIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     TGGIQASQQSLNNIENILTRIEQNIGKADEQQRRFNEDINKGVSNTDRLLGSVKKITG
                     AYMSLKTLGGLGSLSDQMTNTNARLSLINDGQLSDGGLNKMIFQSAERSRASYLDTAQ
                     IVSRIGMNAGSAFSSTREIVSFAEQLNKKFIIAGASTQEMSSALLQLTQGLGSGVLRG
                     EELNAVFESAPNIIKSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAASAETNAQFE
                     KMPLTIGQIFTSIKNNAVMIFGAIQKKIEDTVSSGGFRTFIVNVTDSLYVLGAVGYSV
                     FNGFIDLLSSPTFQGFANMMIVGISLIAQGLGWIITQALSVANVFTQNWSIIAPIVYG
                     IVGAFIAYKVVIYSVFLAQAIYTAITFTQCFVTALFAGELYVATKAMLIHQLMTLGVS
                     RANVTLCATMIMVVSAVALVIAAIFVGVAIFNHFAKTGISATGVVIGAFYFLGACIYD
                     VFAGAWNIVMAFTEFFVNSFNICIYGVQMLFYNLANFVINSMGDVGGSFDRCATTLAN
                     VFINGVNLAIKAVNGLIGLINKIPGVNIDTFGHGYTKNIDSVVGSLKNYQKTLKEPIK
                     PQDWKAPYMDIKNPFDSFNKGYKVGQNLENKLKDAFDINKIAEKAKKDLGLDDLWDKK
                     YGLGDGLGSAGLNSPLSDAAKGAKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQEVINK
                     YTGVNIKIDMNNTNNISKDTDLDGIVNVLTEKLNDAMVVSAEGIV"
     gene            25168..25863
                     /locus_tag="MJF80_001569"
     CDS             25168..25863
                     /locus_tag="MJF80_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBZ0280238.1"
                     /translation="MAYDFYLDGVQLPITPGKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYNIVEDAENASDTKVAIKLKQYRDYSTKKLVPAPPKKTNETGRPSVKIE
                     PKRVDSVNAPNAKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKSMMDKANKGKKVP
                     KYTIYKGQVLRLV"
     gene            25856..27811
                     /locus_tag="MJF80_001570"
     CDS             25856..27811
                     /locus_tag="MJF80_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBZ0280239.1"
                     /translation="MSDELVLANDRDVRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRIEENKTLFDMILTALDDTLRE
                     KKEMYILYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQLFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIDLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSTTSKEDTEMANKINKLLKGKLSGTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKKNGDYMSFSSVDEGIKRGISNLSRNY
                     IHIGRKTLESIRNKYSSSSDKEWVKCVGAFYKQITGSTYNSNSAGTGVGSNEEAEKNL
                     KDVTYQIQGNNQSSSTNNSSKTDKLISIAKSKLGCNYVYGAEGPNTFDCSGFTQWCYK
                     QIGIKIPRTVATQSKAGSAVDLKDRSKWKAGDLLCRVGGGSSNHVMMYIGNGQMIHSP
                     QTGDVVKIQSVDSYRKGKAYTHVRRFI"
     gene            27825..28085
                     /locus_tag="MJF80_001571"
     CDS             27825..28085
                     /locus_tag="MJF80_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBZ0280240.1"
                     /translation="MSQELLQIIKKTAIDAVETSNPMQIAFGTIESLNPLVVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            28090..28509
                     /locus_tag="MJF80_001572"
     CDS             28090..28509
                     /locus_tag="MJF80_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBZ0280241.1"
                     /translation="MIPTDNIDYDIEDVSIINFDVRQEPSKTFKLNIEKNRVDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            28510..29556
                     /locus_tag="MJF80_001573"
     CDS             28510..29556
                     /locus_tag="MJF80_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBZ0280242.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGQLIPIEYVDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKLPDVGGVKVYPVWNGGGTVKLVIINSNFKV
                     PSTDLVNLVQEEIDPNMKGEGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWENI
                     KSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINSIASNLT
                     IDSNSIVVRGEVVG"
     gene            29549..30166
                     /locus_tag="MJF80_001574"
     CDS             29549..30166
                     /locus_tag="MJF80_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HBZ0280243.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNAENKEIKTLHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLVSKDNY
                     MNFGATTITGHEVTIYPWSPSDIETQGEINILSGNGFGYQEVTIY"
     gene            30182..31207
                     /locus_tag="MJF80_001575"
     CDS             30182..31207
                     /locus_tag="MJF80_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBZ0280244.1"
                     /translation="MATDKSYYTILTDIGKAKIANASLVGEKVDFVKIQLGDGGGNEY
                     NPTEEQTALKNIVWEGKVGNVKTDESMTNCLILESLIPASAGGFVVREIGYLDTEGNL
                     LAISKYRSAYKPKVEDGAVIDMKVKTIFVVSNVNNIELKIDPTIIFATLKDLQDLDTK
                     IGTVNTKIDTTKAELTSNIETAKTELNTRIDTENEKQNIKIDQLIAGGSNVAYTQRVA
                     IDDWVEDAENGFKATVTHSLLTQRIVVNIIDVTTKENVVTNFKIIDDNSIEIRSEVKA
                     ELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDRLINIEEKVNGGLSSIATSV
                     NELITYC"
     gene            31224..>31665
                     /locus_tag="MJF80_001576"
     CDS             31224..>31665
                     /locus_tag="MJF80_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280245.1"
                     /translation="MQTEWNFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWHTDAK
                     GGYSKGEITLKKQTTLYVYVGESGFASSSTSNNTKSGFNGGGKGYLNQQVMGTYYSMY
                     GGGATDIRLVGGAWDDEQGLLSRIIVAGGGGGSYSPYTGGAGGGL"
BASE COUNT        12768 a         3354 c         6059 g         9484 t
ORIGIN      
        1 ttaaatggct aatatatatt gtgaaaatta taattgtaaa aactactttg aagatatgtg
       61 tatgcttgaa agaattgaaa ttaataactt caaagaatgc gaaagctatc ttgaaggtaa
      121 aaatgagcta tatgaattag aaaacggata tactatacat cctaaagatt tgaaaatggt
      181 gaaaagtaaa gattattctg ttgaagttac tcatattcca actggtatta cagtaaaatg
      241 ccgttctaca aatagtattt taaaaaataa aaataagtgt ttggaagttc tagaagaaga
      301 actaacaaaa ataaactctc acttagagct agaagattta cgctaaatag gaagtgagct
      361 tatgaaacga agaagatgca gttggtgtgg taagttattt tatcttgaag aaaaatctaa
      421 ggatgtttat tgttgtaagg aatgtaggaa gaaggctaat aagaaaaata aatagtggag
      481 gtattaatat gcaaaaagat gtttggttat atagctggga tgatgagtgc tttggaagtg
      541 atgaatacga aagtaaagag gaagctattc aaggagctaa ggaagaactt agaaggtttg
      601 gggaggtcag tcgattggtt tacgttgggc aaaaagaaga agttaatata cctaatctaa
      661 atgtagagga tgctttagaa catgttcaag atagaattga caatgaattt atggggtatg
      721 gagaagattg gtttgaaaaa atacgtgttg aagatatatt aatactagag aatagaataa
      781 gcaaagtatt taaaaaatgg atagatgaat ttggatataa accatattgg tttgttgtta
      841 gagatacgga agaaatagaa ctaaatgagg tagcaaatga aagttaattt tgtaatagat
      901 ggagagccag ttggttttaa ggaggactat aaatatgaat gagattatga caaatgaaaa
      961 tttaagagtt gtagcagatg atttagttac agtttatgaa actgatacag gagagaaaat
     1021 agtttttgca agagaactac ataataactt agaagttaag agacagttta tagactggat
     1081 tgaagataga attaaacaat atggatttaa ggaaaatgag gattattcag tttttcacaa
     1141 aaatatgaaa aactcaactg gtgggagacc ttcaaaagaa tatgtattga agcttgatgt
     1201 ggcaaaagaa cttgctatgg tacaaaacaa taataaaggg agagaaataa gaagatattt
     1261 tataagatta gaaaagttgc tcaatagaac cttatcaaat tcacagctta gtcaaattaa
     1321 tactattgtg aatgaatcat tacttaagat ggaagttaaa cataatatcc aaatagagca
     1381 acttaaaaaa gagtgttcag aatattatag accaacctct aaaactaaat atgatatatc
     1441 ttcttatata aaagaaagac taggtatatc aaaagttaat gaagaatttg aattagttaa
     1501 gaaaagaact ctattggtat taggtgctga caaatgggag gatatcccga aagatgtatt
     1561 acttaattca ttaaacttaa tagatgaatc aattagaatt ataaaatctg aaagaaagac
     1621 aaatcagatt agtttctttg aaaaagataa tttctgttaa taaaaagaaa aaaggagtgc
     1681 tttcacactc cagtcgtcaa aaatataaaa cttttatcca agattattat aacataaaca
     1741 ggagtgtgga agtatggata ataatatcaa taaaaaagaa ctatttaaaa aagtagaagg
     1801 tagactacat cattataaat ttttaagtgc agaaattaaa aatcttgaat tagatataga
     1861 aagtagagaa aatgagatat ttgggtgtaa ggctgttgga tatgatgaaa aagtaagtcc
     1921 aacatatgct tttaattcaa gtgtggagaa tgagattata aaaaaagaaa gagatattac
     1981 tagattgaaa aaactgaaaa aagataagga aattgaaaag aagaaaatag aaaatgcact
     2041 tacatgctta gatataagag aagaacattt ttttaaactg ttttataata gcagaatgaa
     2101 aaatagtatg gtttatatat ccttagagat gaactcagat agaaaaacat gtagatgtgt
     2161 gagggaaaga ttagtgtata aaattatgga tatgctttat ccaagaatta aggaaaatga
     2221 actaccatta tttaaaaatt agaaaattcc ccagtttttc cccagaaatt cccactttat
     2281 tccctacttt ctcccctttt tgattaaaaa agcatgagat aatagtattg tggaaataaa
     2341 gatttccctc tcaaaactaa ataattgcta ggttagttta aagggctaat ctagcaatat
     2401 gaacagacta ggcagggcat gaggatgctg ttagttcaat tctaactatg ttcaaaacct
     2461 attaatacac tatatgtagc agttgaatta agactaaaat ctcatacaat tttatcttaa
     2521 ttcagagtct aaaaaccgag tggggcttgg taacctcact caccatgcag gtactggtgt
     2581 ttaatctagg ttcgattcct agaacttgcg acataatata tgtatctccc tactaaaaat
     2641 gctaagttta ctccaaactt agcattttac tttttaaaaa gaaaaaagaa atttttattg
     2701 tcataatact atttgtttag gtatattata atgtgcatac ttaaaattaa atacttagca
     2761 agcatttgaa ttaatataca taagatatat gacataattt tttatagtgt aagttattta
     2821 aattaattat aaataacagt aattttatta tataaaatgt acatattgtg aataataata
     2881 ataaaatcat gtacaaaatg ccaactgata attcctcgaa atgtattgca tatttaacgt
     2941 aacgtcagta taattaaatt ataatagtga agtggaaggt ggtacttatg gctacaaaaa
     3001 gtattttaaa aaatgtagat gtaagaaaaa aggcatttgg aagaaatcta gtatcagctc
     3061 tagaaaatgc taaaaataaa caagaaaaag aagttgtatt aagtaaaaaa tgttcagaag
     3121 taccaaaaga taaaataaag gatatattcg ggagatttta atgagtggct atttaattgt
     3181 aaacttaagt aatatgctag gagagctgga ggaagaagaa gttaaaaaaa ttctctccag
     3241 tttttcttgt ccccttaata aagatgtaga agaattttta aaaaacaaag ctattgaatt
     3301 ttctaaacaa ggtttggcta gtacacattt agtgctaact tcttataaag gcaagcctgt
     3361 tatagttgga tatttcactc tagctaataa gtattttaca ataaaaagaa aaacattatc
     3421 aaactcttta gctaggaaaa tagtgaagtt tggacaatac aatgaagaac taagaagata
     3481 tattattgga gcacctttga tagggcaaat aggaaagaat tattcaaata attataataa
     3541 attaatcaag ggtgatgaac ttctaaaaat tgcatgtgac aagataaaag cagtacagtt
     3601 agatatgggt gggaaaatag tgtaccttga atgtgaagat aaacctaaat taattgaatt
     3661 ttataaggat aatggatttg tagactttgg aaaaagaagc cttgataaag acgaaacaga
     3721 ttcgttagat ggggactatt tagttcaaat gttgaaatat ctaaaaaaat aaaagtacat
     3781 aaaatctaaa atgactatct tgatagatgg tcttttttta tacaataaat taaaaggaga
     3841 atgaaattat ggagattaag aaaaacacac aagatgtagt atcagaaaga aaaaattccc
     3901 tagattctga attcaagata cctgcaagtg gtgtgtgtta tatggctgag tttataaagg
     3961 aatctaggga aatcataaaa gaattagata aacattttga aagttgtcta gatgttttat
     4021 ctaaggcaag actctaaata ttttgaatat gctgaatcaa gcatggtttg ccaatctggg
     4081 aaatcagtat ttttaactat aaataaatca aattcattat caggaatagc taaaaagtct
     4141 tcttctgaat tgactatgta attaccaaat gcaagtagtt catcaaaaga atcaaaatta
     4201 gtgtgttggt tcataaattt cttgctaaga attattgtgc gaatcttatc aaagtcaggt
     4261 tttaaatttt gttccattct ttcaattttc ttttgaaatt gctttaaatt tctaatgtca
     4321 atatttttac tcataagaac acctcctttc aatagaatat tagcataaaa ttgtggtgaa
     4381 ttctgctatt gtcgaacgat tgttgaagga tattgtataa taacatagaa tttactatac
     4441 tataaggagg tgattatgtg ggatttgaga taagtggtaa tttgaatttt gacagtgtta
     4501 ttgatgattt aaaaaaagaa gttgaaaata accctactat atttacatca caaaatgttg
     4561 gaaataagtt caaagaaaaa tgtaaaatat gcgaaaaaat atctgaattt gaaatactag
     4621 aagatggtaa agttaaatgt ttagaatgtg ggactgaatt tgaattgaat cttaaagtag
     4681 agtaaacaaa aaaagaatct caattatgag gttctttttt tattcccaaa acaaacaaat
     4741 aaagaggtgg tgatgtgcaa gatgtcaaag aaaaggtaaa acaagattac ttaaaaggaa
     4801 tgaaacaaaa ggaaatatca tcaaagtatg acattagctt aaacacttta aagtcatgga
     4861 taaaaagata caactgggct agtgaaaaaa agaagggtgc acctataaat aaaagaggtg
     4921 cacccttttc taataaaaat tcagttggtc atggtgctcc aaaagagaat aagaatgctg
     4981 aaaagtttgg tttcttttca aaatatctac ccgaagaaac tagggaattg atacaagaaa
     5041 tatctataaa agataaattt gatattcttt gggaacagat aacaattcaa tacgcagcaa
     5101 taataagagc acagaagata atgtatgtta aaggcaagga agaaatggtt aaagaattaa
     5161 agaaatatga aagcacagaa aatggtgaga agatagagta tgaatttcaa tttgcatggg
     5221 ataggcaagc atcttttctt aatgcacaga gtagggctat gagtgaactt agaagtttaa
     5281 ttaaacagta tgatgaaatg attcataagg attggaattt ggctacagag gagcagaaaa
     5341 caagagttga gaagttgaaa tgtgaagttg ataacctaag taaagatgac attggagatg
     5401 atgagttgaa aataagtgta gattatggtg atagaaatga tagttagagt aaattttaat
     5461 ccagatttca aggaagctaa ttttactaaa aaaagataca gagcaatgaa aggttcagca
     5521 gggagtggaa aatctgttaa tgtagcacaa gactatatac taaagttagg agataagaag
     5581 tatcaaggag ctaatctatt agtagttaga aagtcagaag ctacacataa gtattcaacg
     5641 tatgcagagc ttacaggagc tataaatcgt atttatggta aacaagctga taagtattgg
     5701 aaaactactt taaatccctt agaaattaag agtaaagtta ctggtaactc tataattttc
     5761 agaggagtta atgatgcaaa acaaagagaa aaattaaaat caattaactt ctcgaaagga
     5821 aaattaacat gggtttggtg tgaagaagct acagaactta tggaaagtga catagacata
     5881 ctagatgacc gtttaagagg tattttaact aatcctaacc tatactatca aatgacattt
     5941 acatttaatc cagtctcagc tactcattgg ataaaaagaa agtactttga ctataaaaat
     6001 gatgatatat ttactcatca tagtacttat ctacaaaata gattcataga tgaggcttac
     6061 tacagaagaa tgcaaatgag aaaagagcaa gacccagaag ggtacaaagt ctatggtctt
     6121 ggagaatggg gagaaactgg tggagcaata cttaaaaact atgttataca tgaatttcct
     6181 acagaatttg aatactttga taatatgagg ttatcacaag actttggttt taatcatgca
     6241 aatgcaatac ttagaattgg ctttaaggat ggagagttat atatatgtaa tgaaatatat
     6301 gtacatgaaa tggatacctc agaaataata acgattgcaa atagtaaagg tttagaaaag
     6361 agtctattta tgtactgtga tagcgctgaa cccgatagaa ttaagatgtg gaaaagtgca
     6421 ggatataaag ctaaaggagt taaaaaagga ccaggaagta ttaaagctca aatagattat
     6481 ttaaaacagt taagaataca tgtacatcct agttgtataa atacaataaa agaaatacaa
     6541 caatggaaat ggaaacaaga cgaaagaact ggattatatc ttgatgaacc agttgaattt
     6601 atggatgatg caatggctgc tcttagatat tctatagata ataagcttaa aaataatgga
     6661 ataagcttct taaagtaaag gaggtgttaa atatttatat aagtgaaaca gatttaataa
     6721 aagttcagtt aaaaaaagag agcaccttta acctagtaaa agtcatagaa cactacatct
     6781 taaagcatag gccagaaaaa tataaacaag gagaagaata ctattatggt aatactgatg
     6841 taaacaataa gagaagatat tatctcttag atggagctaa ggttgatgat tttactaagg
     6901 ttaataataa agcaattaac aactaccata agcttttagt tgaccaaaaa gtgggctata
     6961 gtgttggaaa tccaatcgta tttaatgcag atgatgataa tctcactaag cttttaaatg
     7021 acttactagg agaagagttt gacgatacaa taacagaact atatctcaat gctagtaata
     7081 aaggggttga atggttacat ccatatatta atagaaaagg tgagtttaaa tatgtaataa
     7141 ttccagctga agaagcaatt cctatttggg atagtaaaag acagagggaa ttagttgcat
     7201 ttattaggtt ttattatatt gaagatatag atggaaataa aataaaaaga gttgagtact
     7261 acacagaaaa tgatgtaact tactttgttg aaagaggtaa tagttttgtt caagaatttt
     7321 tatatgatga atatggaaaa atgactgata tacaagaagg tcattttaga ataaataaca
     7381 aagaacaggg ttggggtaaa gttccattta taccttttaa aaataatgaa aagtgtgtct
     7441 cagatttaac tttctataaa tcattaatag atatatatga caataatatt tctacactag
     7501 cagataactt agatgaaata caagaggtta tttatgtatt aaaagaatat ccaggaacaa
     7561 gtctacaaga gtttatagat aatataagat actataaatc aattaaagta gatggtggag
     7621 gtggagttga taaactagag ataaatatac cagttgaagc taaaaaggag cttcttgata
     7681 gattggaaaa gaatataatt atctttggtc aaggagttaa tccagaatct caaaacacag
     7741 gtgacaaatc gggtgtagca cttaaatttt tatattcact acttgactta aaatgttcta
     7801 agactgaaaa gaagtttaaa aaagcaatta gagagctttt atggtttgtg tgtgagtatt
     7861 taaagataag tggtagtaag agctatgatt ataaaacagt tcaaattact tttaatcact
     7921 ctatgataat aaatgaagct gaaaagatag atatggcagc taaatcaact ggaattgtat
     7981 cagatgaaac tattgtttct aatcatcctt gggttgaaga tgttaacgat gaacttgaga
     8041 gacttaaaaa acagaaagaa gaaagtataa aagaatatga agatgctttt ccgactaaaa
     8101 agaaaactga agaaggtgaa cctttagatg aataatatag attactggat aaagacattt
     8161 aatcagttag aagaagatgt tcataataaa ggagataact ttaacaagga attagaaaag
     8221 caatatgata tagtacttta taatattctt agggaaataa ataattggta tataaaattt
     8281 gctaaggata ataaaataag tatgcaagaa gctgaaaagt tattaaatct aagagaatta
     8341 gctgaactta aattatcatt agaagaatat atcaagtatg gagaagaaaa tgtaattagt
     8401 caaaagtgga tgaaagaact agaaagtgca gttaaaagag ttcgtataag cagactaaaa
     8461 gcattagagt taaaaataag gcatcaagtt gaggtattat actcaaaaga gtatgaggat
     8521 gtaaatacgc ttatagcaga cacttatcaa gatagctatt atcatactgc atttgaaact
     8581 caaaaaggta ttggaattgg attaatgata gccatgctta gcatgaataa tattaacaaa
     8641 atagttagta gtccttggac cactgatgga ataacatttg gtaatagaat ttggaataag
     8701 catagaccaa ggttaatgag tgaacttaac aaaggattaa aagaaacatt aattaatggg
     8761 atatctcctg aaaacttaac gaataaaatt aataaaaatt ttaatacaag caaagaagaa
     8821 gctaagttat tagtaaaatc tgagcttgcc ttttttagtt cattaagtca aagggattgt
     8881 tttaatagtc taggtgttga gaaatatgaa atagtaagtg cattagatag taaggtttgt
     8941 gagaaaagat gtagtcctct tgatggtaaa gtaattgaaa tgaaatacta tgagatagga
     9001 gttactgctc caccatttca tcctagatgt aggtgtgtaa tagtaccata ttttgatggt
     9061 gaagaaagtt atagagctgc aagagaagaa gatggtggaa cttattatgt accatctagt
     9121 attaagtata aagaatggca taagaagtat gttaaaaaca cttactaagt tagtaggtgt
     9181 ctttattatg taaaaaataa aatttattga gagggtgatt tgaaatgctt aaattatata
     9241 ttttatcaat aattgtattt tgcacagggt tttatttgta tatgttaagg cttggaaatg
     9301 ttaaagaagt aatcgaagct ttaaaaaaat ataaggcttg gaaaaactat aaacttattt
     9361 ttatagcttt gtttccattg ttaaacttta ttattggttt aatatttata gtagtttctt
     9421 tatttgcaac gaacaaggat ataatagaaa gtttggagga tgaataaatg gctagatttg
     9481 taaagaaagc agttgaagtg gaagctttta aatttgatat agatatagaa ccaccaatat
     9541 ggatacgaaa agagattgaa cgtggcaatg cttgggttga acactctatt gataaggaac
     9601 atactaaaat gttcataaga actattgaag ggctaatata tcaagttaac ataggggatt
     9661 acattataca aggagtaaaa ggagaaatat atccttgcaa agctgatata tttgaaatga
     9721 cttatgagaa agttgaatat actgcaacta ttgaaaattt aacaaactat gctgaaaatt
     9781 tagaaccagg gcataaatat attgacaaag gaagtagcaa gaaaaatagc ttagaatttt
     9841 cagttaaatt aaaactagat acaaaagact ttgataaaaa tatagaaaat gctacaaaag
     9901 aaattgaaac atttaatgaa gcagtaggca gactagagaa aaaaataaat aggatatctg
     9961 caaaagaaaa taaagttgat atagataaga ttgtaaaaca attagaagaa agtctaagag
    10021 aacgtattga ataagttttg ggggatggaa atatgtttaa acagaaatat attaagaagt
    10081 caaataaaat aagtgctaga aatattatag catttattat tacagttatt ggtataactt
    10141 taggtatttt tataggtatc aacataatca tggctcatgt tttaggaata gctaacatgg
    10201 tagataataa tacttttacc tgtgttagat tagtttatgg cttagtagga gttataagtg
    10261 gatatttaat aggaaaagca atatacctta tagcttcact aataagctat attatttatg
    10321 aataatttgt tcaaagattt tttatcatgt aaaaacaaaa ggaggcttat tttattgaaa
    10381 gagtatataa tttggtttaa aagtggcaat agcatatctg gaatagtaga tgaagatgtt
    10441 gctgataagt taatgcaaga ttttattgaa gctgactcag attgtaggaa cttgaaagga
    10501 tatttagatg aagatggaac aacaataata gatttatcac aaatagaagc tatatcaata
    10561 aataattgta gcgagaataa taacattggt tttaatccta gataaggctt ttttattatg
    10621 taaaaaatga aaggagacat ttaaaatgga ttggttaaaa gaattattgg aaggaataaa
    10681 aatagaggat aacaagattg atgtagcgtc tcttcaaaaa tctatagaaa agaaaataaa
    10741 agagactaca gttactcaag aagattatac aaatcttgaa acacagctta atacagctaa
    10801 tgaaactatt aaaaagtttg aaggaggtat gacaaaagaa gatgtagaga atctaaaaac
    10861 aacttatgaa actgataaga aaactttgga agaaacctac aaaaaagaaa ttgaagaaaa
    10921 ggactttaat tactggttaa atgatgcttt taagtctgtt aaatgtaggg atgaaatagc
    10981 attaaaagct catttagaca tggaagcact aagaaatagt aaagatagac aaaaagcttt
    11041 tgaagagcaa ataaaccctt tgaaacagga taaagattat ttgtttaatg caacactaga
    11101 aggtgaagaa cctaaaatag atactataac accaggacaa gagcctaaga taaatgattt
    11161 tggttttaat tttactgggg taagacctca tgaaaataat aataaatagg aggaaataaa
    11221 atggcagcac taaattatgc aaaagaatat tcaaatgttt tagcacaagc atatccttat
    11281 actttaaact tcggggattt gtatgcaaca ccaaataatg gaagatatag atggactggt
    11341 tctaaaacaa tagaaatacc aactatatct acaactggaa gggtagattc aaacagagat
    11401 acaatagcag tagctcaaag aaactatgat aatgcttggg aatctaaaac attaactaac
    11461 caaagaaaat ggtctacatt ggttcatcca gcagatattg accaaactaa ctatgtagca
    11521 tcaataggaa atataactca agtatacaat gaggaacaaa aatttcctga gatggatgct
    11581 tactgtatat ctaaaatata tgctgattgg atagctctag gtaaaacagc agagacaact
    11641 gctcttacaa cagcaaatat cttagaggtt tttgataagt taatggaaaa aatgacagaa
    11701 gctagagtac ctgctgttgg aagaatatta tatgttacac caggagttga cactttaatc
    11761 aagaatgcaa aagagataca aagaactgta aatataaaag atggaggaac ttctcttaat
    11821 cgtcaaacaa ctgatattga ttcagttaag atagttaaag tgccatctaa tcttatgaaa
    11881 acagtatatg actttactac tggatggaaa gcaggtgcag gagctaagca aatatttatg
    11941 tctttaatac atccaagtgc aataattaca ccagtttctt atcaattttc tacattagat
    12001 gaaccaagag cagttacaga aggaaaatat ttatactttg aagaaagttt tgaagatgta
    12061 tttatattaa ataaaaaagc tgatgcaata caatttgttg ttgaaggagc tggagcataa
    12121 tggcacaagt aaggaaatta aatagaatat taactataga agaatgtaaa atagatgatt
    12181 tcttagaaat gggatatgat ttaatagata aagctggaaa agtagagaaa tatggtaagt
    12241 ctttaaatgt aaaggatttg gttgctgaaa ataatatttt aagaaacaaa gtagaatctt
    12301 tagaagtaga aaataagcag cttaaagaga aagtaaaaac tttagaggaa gaagatatcc
    12361 aatctataga aaaagcagag cctacagaaa agaataagtc taacaaaaag taggtgaaaa
    12421 ttatggaaaa taatctgatt gatgatatag aaaaaagact tgaaagtttt ggatatatat
    12481 taaaagatgg ggataagtgg ttaataggtt ttgtaagaga aaagatagaa aatattatta
    12541 agctggattg taatataaaa actatgccaa ttgaattgaa agaaattgaa gttgatatga
    12601 tagttggaga gttcttattt accaagaaaa atatggggca attagatata gaaagcatta
    12661 actttgaagc tgtagaaaag tctatatcag aaggtgatac aaaggtagat tttgctatag
    12721 gaagtggctc tcaaacacca gaacaacgct ttgatagctt agtagcttat cttactactt
    12781 atggtaagaa taagatatta acctttaggt gcttaagatg gtaagtaaaa ctagaaaagc
    12841 aatagaaatg ttatatagag ataaatgcac tatagtagaa tatcaaccaa tcaaagacgc
    12901 tattacaaaa cgaactaaca ataaagaagt aattgtatta gagaatcaac catgtaaact
    12961 ttcatataaa aatatagttt ctgctacaga agggaaagta gctaagctag agcaaactat
    13021 taaactcttt atatctccag atatagaaat taaagcaggt tcaaaactta ttataaatga
    13081 taaagagtat gtaagaagtg gagaatcagc tatatatcca aatcatcaag aaataatact
    13141 tgagttattt aaggataaag cataatggct agatggggca gtgttgattt tagagagttt
    13201 aaaagagttt gtaaaaagat ggaggagctt acaaagattg atttagataa gttttgcaag
    13261 gatgcagcaa gagaattagc agcacgatta cttgggaaag taattagaag aacaccagtt
    13321 gatacaggat tcttacgaca aggatggaat ggagtggctt atgctaggtc gcttcctgtg
    13381 tataaacaag gaaataatta tattatagag gttgttaatc cgactgaata tgccttaaca
    13441 caatggggca tataaaaccc agcaaaatcg gtaaacgcta agtgtaatag tgttacatta
    13501 taaagtaggt agaatatgga catagaataa ctcttgattt ataatgtata aaagaggtga
    13561 ttttatggca aaatttttag atattacagg aaaaaaattt ggaaggctaa gagtcattaa
    13621 attttctaaa gagataaaaa gtggtaaaag aaatagaaaa tattggttat gtaagtgtga
    13681 ttgtggaaat ttcaaagaaa taagaactga ctctttaact agtggcttag tacaatcttg
    13741 tggatgttta aaaaaagaac aagataaatt aaatttaaca gacaaatacc aatttaaaaa
    13801 gaaatataag gttcaaaata aaagacttta cagtatatgg aagggtataa tatctagatg
    13861 tacagataaa aataataaaa gatacaatag atatggtgaa aggaatataa ttgtgtgtga
    13921 tgaatggttt tgctatgata attttgcaaa ttgggcattg agtaatggat attcagagaa
    13981 acttacaatt gatagaataa ataatgaagg aaattatgaa tcaagcaatt gtagatgggt
    14041 ggatataaag acacaatgta gaaataggtc aacaaatata ttagtaaagc atgaagaaaa
    14101 agaaattaca ttgatagagc tttctgaaaa aacaggtatt tcatattctt gcttaagaag
    14161 tagatattct aagggattag taggaaataa gcttatagaa aaagttaaga ttattgaaga
    14221 aagcagagct aagttatcta ttgaggatgt aaaagaaatt agaaaaaagt attctgatgg
    14281 atatacaata aaacaactaa gtgaaatata tcctgtaaca tattcatcta tatcaaatat
    14341 agtccataga agaacatgga aaaatattta attaattata tatgccaata ccgaggaaaa
    14401 cctatagatt gcgaataggc tataggtttc cgtagagcgt agagagtgaa taaatataat
    14461 ctctccaaga gtgctgggca actaaataaa gtttattttg ttgatgatgt acgccgaact
    14521 tataggaaac tataagagct agaggataaa aagcctttag gataacaaaa tgcatatgtt
    14581 gaatatggcc atagaactaa agatggaaaa ggttgggtta aaggacaaca tttcttaaca
    14641 atttcagaga tggaactaca aagccaagtt gataagatta tagagaaaaa actattaata
    14701 ttgcttaaag gagtgtttga tgcttaataa tattatagat ggaatatcag taaagttaga
    14761 taaatcgttt ggagaaaaat atacaattta tagcgaagat gtggagcaag gtattaatga
    14821 accttgtttt tttattgttc ctttaaatcc aagcaagaca ccatatccaa gcgggagaga
    14881 attaaagaaa aattcttttg atgtacatta tttccctcat tcagaagata agaattttga
    14941 aataaatgag atagctgaga tgctactgga ggaattagag tatatagaaa ttgatggaga
    15001 tttagtcaga ggtacaaata tgaattttga aattatagac aatgttcttc acttctttgt
    15061 tgattataac tactttacta taaaaagtaa tgacacagat aagatggata cagtagagtt
    15121 attcggtggt ttgaagagag gtgataattt tgagtaaaac attaagcaaa gaagataact
    15181 acaagtttac taaggagcag atagttaact ctaagaagta tgtaaataga aaagatttat
    15241 taaatgcaat tttaaaagaa aatgatttat attccttctc agaggtagag gatagaataa
    15301 ataaatttat gaaaggagtg agttagatgg ctttaggtgg aggaacattt gtaacacaaa
    15361 ataaggtcct acctggtgca tatataaatt ttgtaagtgc tacaagggca accagttcat
    15421 tatcggatag aggtattgtt gcaatacctt tagagttaga ttggggcata gatgaagacg
    15481 tatttcaagt aaccagtgat gattttgaga agtattcagt gaagtatttt ggatatgatt
    15541 atactcatga gaagctgaaa ggtttgagag atttattcaa aaatataagg ttgggatatt
    15601 tttataaatt aaataaaggc gttaaagcca gttgtactat agccacagca aaatatagtg
    15661 gtatcagagg aaatgactta aaagtaacag ttacaacaaa tatagatgat aatgctaagt
    15721 ttgatgttgt aacactttta gataataaga aggtagatac tcaaatagca aaggttatta
    15781 cagacttaca agacaatgac tatatcactt ggaagaagga tgcaacacta gaagcaagtg
    15841 caggacttgt atttactggt ggaactaatg gcgaagctgt gacaggagca gagtaccaag
    15901 ctttcttgga taaaatagaa agctatagct ttaatgcttt aggatgtttg gctacaacaa
    15961 cagaaattaa aagtttattt gtagaattta caaagagaat gagagataag gtaggagcta
    16021 agtttcaaac agtactatat aagaaaagtg atgcagatta tgaaggtgta gtgtctgtag
    16081 aaaataagat taaagatatt ggattagtag aatctagttt aatttattgg gcggctggag
    16141 ctatagcagg atgcgatata aataaatcta atactaataa aaagtatgat ggtgagtttg
    16201 atgttgatgt taattataca caaatacaac ttgaagaagc tttaaaaact ggtaaattta
    16261 tattccacaa ggtgggagat gaagttcatg tgttagagga tataaatact tttgtatcat
    16321 ttacagatga taaaaatgac gatttttcaa gtaaccaaag tgttagagta cttgaccaaa
    16381 ttgctaatga tatagcgact ttatttaata caaagtactt gggtgaagta ccaaatgata
    16441 aatctggtcg tatctcgttt tggaatgatg tagttaagca tcatgaacaa ctgcaaaata
    16501 tgagagcaat agaagatttc aaagctgatg atgtttctgt agaacctgga agcgataaga
    16561 agactgttgt agtaagtgat gctgtaaaag ttattagtgc tatgagtaag ctttatatga
    16621 ctgtttcagt tagttaagag aggagtgtga taaatagatg tctaaaaata ttactatgaa
    16681 tgttagagat gcaataagtg gttctaaagc tgaatgtttt gtaacaataa aaggtaaaag
    16741 atataatttt atgcaagcta ttaatttaga agcaaaaatg gaaaagaata aaagtgagat
    16801 gccgatttta ggcagcatta caaaaggaaa caaaagtaca ggaagtaaat tttcagggag
    16861 tgcaacattt tattataata cttctatttt tagagagttg ttatatgaat ataaagaaac
    16921 aggcgaggac atttactttg atatgcaaat taccaatgaa gaccctacat caagtgtcgg
    16981 aagacaaact ataatacttg aagattgcaa cctggatagt ggaataattg ctaagtttga
    17041 tgctgatggc gagtatttgg atgaggatgt agattttact tttgagaatt ggagaatagt
    17101 tgagaagttc aatatagcaa atggtatgga ataagataca catttataaa ttatagatgt
    17161 gtatttttta tttacaaaat taggagatga ttaaaattaa agataaatat gagataaaag
    17221 attcaatttc ttttgattat agtaaaaaaa gaactataga agaatgtgtt agtgaaatat
    17281 ataaaaaagc agggaaatat cttatagata ttgctgataa actggcactt gatacaattg
    17341 aaggttcatc attaaaacca attacaataa aaatcaaact agatgaggat ggcattgcaa
    17401 caatagaaaa agaaacaaaa tatttaatca tggaggtaga ataatatggg agatttaaac
    17461 gcttttttaa gtcaaaatgc aataaaagta gagaatagaa agtatgtggc aagtgaaagg
    17521 tttgtaggtg aagatggaaa accagtagaa tgggaactta aagcaataga ttcagataga
    17581 gatagacagt taagaaaaaa ttctgctatc agagttcctg ttataggcaa aaaaggaaaa
    17641 gcaacagggc aatacactag tgaaacagat tttaatactt acactttgaa attatgtgtg
    17701 gaaactatag tatttccaga tttacatgat gcagaacttc aaaatagcta tggtgtaatg
    17761 ggagcagagg aactattaac aacaatgtta actcctggcg aatacacaga cctttcaagt
    17821 gaagtaggag aggtaaatgg ttttgataga acttttgaag ataaagtaga agaagcaaaa
    17881 aactaattga aggaggcgat tatgatgcta gtgtagctca ttattgcctt cataaattca
    17941 aatggaaacc acatgaatat acagatttac cagactacga gagggcattt gttgctgctt
    18001 ctatagatat taaagtagaa gaagaaataa aagaagaaaa aaagactgct aaagaagcta
    18061 gaagaagtag aagaagataa aatataggta aaatatgtaa gaattatatg ttataatatt
    18121 tttagcaaga agatgtaatc tacaatttat agagtggagt tcatacaaaa gattatcctc
    18181 ccaacgtata gaagggaggt gaatatgtat ggataatttt ttacaaaatg tactagaaag
    18241 actatctgtt agtttaatag tttttctagt tagcaattta cttaaaaaac gtaaaaaacc
    18301 actcaaagct ggcactaaga gtggttggga atttgattta aaaatcagat tccataagtt
    18361 caaataagta ttatgaactt cactctactg caaatagatt gtagttcttc ttgtttttat
    18421 tataccacaa aaaattataa ataaaaccgt tgtttatata aaatatttga aatgatagca
    18481 cattcccgtt ttgggaacat ggtcaaaact caaactatat tactggtatt atattaaata
    18541 tttagcatca aaattaaata aagcacttac tttttggtag gtgctttttt atattcaaat
    18601 ctgtgtactt aattgaaata tttggtaaaa tatgtaagaa ttatatgata taataattgt
    18661 agcaaggaaa ataaaattga aaagctaagg gtgattttct catataaacg ccaaattcca
    18721 atataggaag gaggtgaatt tatatgttaa ttaacttttt attgagtata gtagctggaa
    18781 ttgtttcagc ttacatttac gagaaaataa aaaatcaccc aaacgccaat aagggtgatt
    18841 taaagaaata acttttaaat tctaatcaat tagagaaatt caccttttac aaagtaaatt
    18901 ttatctcctt gctattatta taccacaaat tggtacagat attcaaaaat aatatattta
    18961 tgatataata aaaatgtaga gattttgcag tgttcgattt ttgtgataaa atatggccta
    19021 acacttgaaa tataatgtat tgaggacgta tgataagtat tatcatttgc actactcatg
    19081 gttcactgca aatttgagag aggtgtgtat gtgtaagtct tggaaatact caatttattt
    19141 tggggtttta gattaactat atggaatgta aattattcta cagttgatac tatgcttaag
    19201 cgcgggtttt agattaacta tatggaatgt aaattttctc ctaatttagg attaaacaaa
    19261 gaggttaaat agttttagat taactatatg gaatgtaaat attaatagaa ctggtccgag
    19321 tgctgctgct aatcctggtt ttagattaac tatatggaat gtaaatataa atgtatttca
    19381 tacaggaata tttttctgca acagttttat attaacaatg tagataaaaa ttaaataaag
    19441 aaaagaaagc acttacaaac atgtaggtgc ttttgttttg ctcaaattgg tcgattgggt
    19501 aaaataatta gaaaaaattg ggataagtta ttgacttttg agttccaaaa ctatataatt
    19561 taattatgga actcaaaagt gaggtgataa catgagtcct aaaaaaatag gaagacctgt
    19621 tgtaggaagc cctaaaacta atgatattaa ggttagagta gatgatgaaa ctaatgaaaa
    19681 attagatgaa tactgtaaaa aaaataacct tacaaaagca gaggctataa gacagggaat
    19741 ccatttactt ttagaaaaat aacaaaaata gagtgttcgc ccgaccaagt ttgaacactc
    19801 tatttaaacc ataagaagta atcttctata tgaaatattt tatcatatag agaaacttct
    19861 ttcaaattaa atttaggagg agtatgtatg atgaatgatt tacaaatatt taaaagtgaa
    19921 gattttggag agataagaac agttgatatt gataatgaaa tttggtttgt tggtaaagat
    19981 atagcagaag ctttgggata ttctaataca agagaagctt taaaaactca cattgataat
    20041 gatgatatag ctgaggtcgt gattcacgac ggcagccaaa atagaaatat gaaaataacc
    20101 aatgaaagtg gactttattc tctgatattt gggagcaagt tagagacagc taaaaatttt
    20161 aagaattggg ttacaaaaga agttttacca gctattcgac aacatggtgc atacataaca
    20221 aataacgctg accctcaagc attaagagaa aaagcaaatg aaatagaaag tttagataca
    20281 gttaataaga ctatagaaat actaacacct tttcttgata atgctggaat agatgaaaaa
    20341 gcaaagttac ttacagcaaa gactatctac aaaaaagcag gaatagagtt gcctttggag
    20401 attgaagaaa aagaacattt ctttgataca aaacaaattg caactaaatt aaatatgtat
    20461 tctaagtcta ataaacctgc atttcatgca ataggtgaaa ttattaagaa gttagatata
    20521 caagataatg aaaagttagt agtactagag agtaaaagag gttggagtgg aagtgtcaat
    20581 aagtattcac agagtgtaat agataaaata agacattgga taggtgaaaa caataagcct
    20641 gctaagatac aaggtgagaa gaagaatttt catgtggttt ataagattga gtaaatttat
    20701 aagattgtat taaataattt attttagttt attttggggg gattaataca atgattgaag
    20761 atttacttaa tgaatataat ttaaaaactg atgaagatgt agagtatttt gtgaagtttg
    20821 ctaatgcatt atatgaacta aaacagaata gtgaagaaaa atttcaagaa tatgcagaga
    20881 tattaagagg tattcttaga gaacaagaag agagaaaaaa taagtaaaat aatatagata
    20941 aagcacttga atattatgat gtttcaagtg ctttgtttgt taaaaaatga tataatatag
    21001 gtaagagtta tattaacaat gtggtatgta aagtgacttt catatgctct tttatcagct
    21061 tctgacataa gttttatatt aactaagtgg tatgtaaaga ctggtaaaac tcttaatgta
    21121 tatgttattt gtaaagattt tatattaact atgtggtatg taaagcagct ttttctttta
    21181 agctacataa ctgattatac tattttatat taactaagtg gtatgtaaag ttcataactt
    21241 gatttgattc ataacttgta gccattgttt tatatcaact atgtggtatg taaattgtct
    21301 tattttatta ttaaagtttt cttgttcatt tgtgttttat attaactatg tggtgtgtaa
    21361 aggaattagt agagtacaaa aatttaaagt cagaatttag tattatatta actaagtggt
    21421 atgtaaattc aaatataaaa gactttacat ctttgccatc gattggtttt atattaacaa
    21481 tgtggtatgt aaagaaggaa cttttaagaa gaatgttact tgcaggagaa attttatatt
    21541 aactaagtgg tatgtaaagt ccacatctgc atctttactt atgttatttg tattgtgttt
    21601 tatattaact aagtggtatg taaagtcatt tatcatgttc aatctagcat tcgtactagt
    21661 cagttttata ttaactaagt ggtatgtaaa gaatggagct atagtagaca gcattgatac
    21721 aaatattatt ttatattaac taagtggtat gtaaagtttg caatagcttg tttcataaga
    21781 tatacgcatt gagttttata ttaactatgt ggatttaaaa ttaaaaataa tgaaaaacac
    21841 ttacttgaat agtaggtgtt tttttattga aaggatgtga taataatgta aaaattttac
    21901 tgatatagta taataatctt ataaaattat gtagggggta atattatggg attatttggg
    21961 ggtaaagaac catgcagcat atgtggagga aagggtaaaa ataaagtttt agaaaatgaa
    22021 tatttatgta ataattgttt tattaatttc acaatatttt ctagagaaag attaaaagca
    22081 actagtgcaa tgcaagtatt agctgaccat gaagggataa gaaaatttat aaatttttct
    22141 aaaacaaata gagaactatt agcaaaattt gttgagacaa atagaattaa taaatttgta
    22201 tccattgatg aagataataa ctttattaaa atatcagata ttcgcaaagg tggagatgta
    22261 atagagaatg tatatgcaat tgatgaaatt atgcaatttg aacttttaga agatggagag
    22321 tctatagtta gtggaggatt gggaagagct gtagttggag gagctttgtt tggtgctact
    22381 ggagctatag ttggtggaat aacaggcaaa aaaacaacaa gaaaagttgt tgatacattt
    22441 aaaattaaaa taacctttaa taatatcaat aatcctttgg aatatgttaa tttgattaat
    22501 acaaaaacaa aaactaattc atctatatat cagaatgctt gtaatgaagc acaagaaatt
    22561 ttatcagtac tttcaataat aataaaaaat aatgataaga ctgataatac acaaagtaaa
    22621 actagtgatt ctatagaaca ggttaaaggt ttaaaagagc tactagactt aggagcaata
    22681 acagaagacg aatttaatac taagaaaaaa gaattgttga atttgtaata ttataaaaca
    22741 cttacttatg taggtgtttt tttatataaa aattttagaa aggagagtga aaatatggct
    22801 acaatacaaa catcaattcg aattttcgat ggaatgacac ctgcctttag gaatatgact
    22861 aattctatta atacaacaat taatagttta gagagactgc aaggaagatt aaataatcca
    22921 cttaatacag gtggtataca agcatctcag cagagcttaa ataatattga aaatatctta
    22981 actagaatag aacagaatat aggaaaagca gatgaacaac agagaagatt taacgaagat
    23041 attaataaag gggtaagcaa tacagataga ttgcttggaa gtgttaaaaa gattactgga
    23101 gcttatatga gcttaaaaac attaggagga ttaggcagtt taagcgacca aatgactaac
    23161 actaatgccc gcttgagtct gataaacgat ggtcaacttt ctgacggagg gttaaataaa
    23221 atgattttcc aatccgctga aaggtctcgt gcatcatatt tggatactgc acagatagta
    23281 tcacgaatcg gaatgaatgc aggaagtgca tttagtagta ctagagaaat agtaagtttt
    23341 gcagaacaat tgaacaaaaa atttataata gcaggagcta gtactcaaga gatgagttcg
    23401 gcacttttac aattgactca aggtttgggt tctggtgtgt tgagaggaga ggaactgaat
    23461 gctgtatttg agtcagcacc taatataatt aagtcgattg ctgactactt agacgtggat
    23521 ataggaaaaa ttagaggaat ggcaagcgag ggaatgttga cagcagatat tgtaaaaaat
    23581 tctctgcttg cagcatcagc agaaacaaat gctcaatttg aaaaaatgcc acttacaata
    23641 ggtcaaatct ttacatcaat taagaataac gcagtaatga tatttggagc gatacaaaaa
    23701 aagattgagg atacagtctc aagcggagga tttcgaacct tcatagttaa tgtaacagat
    23761 tcgttgtatg tcttaggagc tgttggatat agtgtcttta atgggtttat tgatttgctg
    23821 agcagtccaa catttcaagg ttttgcaaat atgatgattg tcggaatcag tttaattgcg
    23881 caagggttgg gatggataat aacacaagca ctaagtgttg ctaatgtatt tacacaaaac
    23941 tggagtatta ttgcacctat agtctatggt attgtaggag cttttattgc ttataaagtt
    24001 gtaatatatt cagttttttt agctcaggca atttatactg caattacttt tacacaatgc
    24061 tttgtaacag cacttttcgc tggagaatta tacgttgcta caaaagcaat gcttatacac
    24121 caattgatga ctttaggagt aagcagagct aatgtcacac tatgtgcgac tatgattatg
    24181 gtggtatcag ctgtagcctt agtaatagca gcgatattcg taggagtagc tatattcaac
    24241 cacttcgcaa aaacgggtat aagtgcaact ggagtagtaa ttggagcttt ttatttcttg
    24301 ggagcttgta tttatgacgt attcgcagga gcttggaaca tagtaatggc atttacggag
    24361 ttctttgtta actcatttaa tatatgtatc tatggtgtac agatgctatt ctataatctt
    24421 gcaaatttcg ttataaactc aatgggtgat gttggaggtt ctttcgatag atgtgcaaca
    24481 acacttgcaa atgtattcat aaatggagta aatttagcaa taaaagcagt aaatgggtta
    24541 attggattaa ttaataaaat acctggagta aatatagata cctttggtca tggttataca
    24601 aaaaatatag actctgtcgt tggaagttta aaaaactatc aaaaaacatt aaaagagcct
    24661 attaaaccac aagactggaa agcaccatat atggacatta aaaatccatt tgactctttt
    24721 aataaaggat ataaagtagg tcaaaattta gaaaacaaat taaaagatgc ttttgacatc
    24781 aataaaatag cagaaaaggc taaaaaggat ttaggactag atgacctttg ggacaaaaaa
    24841 tacggtcttg gtgacggact cggttcggca gggcttaatt ctccgctcag cgacgctgca
    24901 aaaggagcaa aagatacagc aggaaacaca gctaaaatgg caaagacaat ggataaaagt
    24961 caagaggact taaaatactt aagagatata gctgaacaag aagtaataaa caagtataca
    25021 ggagtcaaca ttaaaattga catgaacaat acaaacaaca taagtaaaga tactgactta
    25081 gatggaatag ttaatgtatt aactgaaaaa ttaaacgacg ccatggttgt atcagcagaa
    25141 ggaatagttt agaaaggagg gatataaatg gcttatgatt tttacttgga tggagtgcaa
    25201 ttaccaatca cacctggcaa attagagatt aaagtaacaa acaaaaataa aacagttgat
    25261 ttaataaaca ctggagaagt aaacatacta aaaaaagaag ggctatctga aataagtttt
    25321 gaagcagagt ttacacataa taaattgcct ttttgtagag gtcaatttag agatgttcaa
    25381 ttctttttaa gtaaactgga attactaaag acagattgta agccatttca atttattgtt
    25441 tcaagggaat taggtaataa agtactattt aacactaata tgaaagtgtc attagaagaa
    25501 tataacatag tagaagatgc agagaatgcc tcagatacaa aagttgcaat aaagttaaaa
    25561 caatatagag attactcaac taaaaagtta gttcctgctc cacctaaaaa aactaatgag
    25621 actggtagac caagcgtaaa gatagagcca aaacgagttg attcagtcaa tgcaccaaat
    25681 gctaaaacat acacagtcaa ggcaggggat agtctttggt caatttgcca aaaacagtta
    25741 ggtaatggct cactgtataa gaaggtatat gagttaaata aatcaatgat ggataaggct
    25801 aacaagggca aaaaagtacc taaatatacc atctataaag ggcaggtgtt gaggcttgtc
    25861 tgatgaatta gttttggcaa atgataggga tgtaagatta gtcatagcac attgggaaga
    25921 tttctacgaa cctgtagttt tggatggtat cacatgggaa attgaaaggc gaggaacacc
    25981 ttcaaagtta gaatttacaa tagtcatgga tgatatatta gagttttgcg aaggtaactc
    26041 agtaagatta tattacaaag gtgtaggcat attttatgga tatatatttc agaagaaaag
    26101 agataaagaa aatcacatta aaattgttgc ttacgaccag ctaagatatt ttaagaataa
    26161 agatacttat gtatatagca ataaaactgc aagtgaactt gtaaaaatgt tggctaaaga
    26221 ttttaattta aaatacaatg tcatagaaga tactaagtat aaaatatcta ggatagaaga
    26281 aaataaaaca ctctttgaca tgatactaac tgcacttgat gatactttaa gagagaaaaa
    26341 agaaatgtat atcttgtatg atgattttgg aagaataaca ttaaagaatg ttgcatcaat
    26401 gaaactggat actgtcatga acaatgatgt aattgaggat tttgactata attcatcaat
    26461 agatagtgat acttacacaa agattaaact tgtgagagat aatgaagaaa cagggaaaag
    26521 agatgtatat attgcacaag actctacaca tatgaggagt tggggaatac tacaactatt
    26581 tgatacagta gataaaaaca tgagtgaagc agagataaag caaaaatgtg atatacttct
    26641 aaaactatat aataagaaaa ctaagtcatt aagtttaaaa aatgtgttag gagatattag
    26701 agtaagagca ggttgtttag tacctgtttt tttagattta ggagatattg atttacaaaa
    26761 ttatatgtta gttgagaaag taaagcacac atttgaaaat aattcgcact ttatggattt
    26821 gactcttgtt gatggagatg aatttgcttc ttattcttca agctcatata gtagtggaaa
    26881 tactaataat aaagatgaaa agaaaaatgg tcctgcacaa agtactacaa gtaaagaaga
    26941 tactgaaatg gctaataaaa ttaataaact acttaaaggt aaattatcag gaacaggaaa
    27001 tatatttgtt aaatattcaa atgcttataa agttaatcca gcattaatgg cggctatatc
    27061 tatgcacgaa tcagctagag ggacttcaaa tattgcaaat actaaaaata atttctttgg
    27121 aatgaaaaaa aatggagatt acatgagttt ttctagtgta gacgaaggaa taaaaagagg
    27181 tataagtaat ttatcaagaa actatatcca tataggacga aaaactttag aaagcatcag
    27241 aaataaatat tcttctagtt cagacaaaga atgggtaaaa tgtgtaggtg cattttataa
    27301 gcaaataaca ggaagtactt ataattctaa tagtgcaggt acaggagttg gaagtaacga
    27361 agaagcagaa aagaatttaa aagatgtaac ttatcaaatt caaggcaaca accaaagcag
    27421 tagtacaaat aatagctcta aaacagataa attaattagt atagcaaaaa gtaaactggg
    27481 ttgtaattat gtgtatggag cagaaggtcc taatactttt gattgttctg gattcactca
    27541 atggtgttat aaacaaatag gtataaaaat tcctaggact gttgcaacgc aaagtaaggc
    27601 aggcagtgca gtagatttaa aagatagaag caagtggaaa gcaggagact tattatgtag
    27661 agttggtgga ggtagtagta atcacgtaat gatgtatatt ggaaatggtc aaatgattca
    27721 ttcaccacag actggtgatg tagtaaaaat acaatcagtt gattcatata gaaaaggaaa
    27781 agcatataca catgtgagaa gatttatata agtgaggtga taaagtgtca caagaattat
    27841 tgcaaataat taagaagact gcaatagatg cagtagaaac aagcaatcca atgcagattg
    27901 catttggaac tatagaaagt cttaatccat tagtagttaa gatagaacaa aaactatcta
    27961 ttggtgaaat ttttctaata caaacagata catttaaaag atatacagat aaaaagatag
    28021 gagataagtt agttttaatt cgtatgcaag gagggcaaca atacttgatt ttagatagga
    28081 tgtgatgaag tgataccaac agataacatt gactatgata ttgaagatgt ctcaataatt
    28141 aattttgatg ttagacaaga accaagtaag acgtttaaat tgaatataga gaaaaataga
    28201 gtagatggta tttgtgatga tgtagaagca ttaaaacaaa ccatcttttt gattttaaac
    28261 acagagagat accaacatct aatctatagt tggaattatg gagtcgagtt gaacgacctt
    28321 attggagaac ctatttcata tgtaataccc gaacttgaaa gaaggataac agaagcacta
    28381 attcaagatg ataggattga aaatatagat aattttgagt ttcaaaatat aaagggtaaa
    28441 gtacaatgta gattttcagt tcatacaaaa tatggaaata taaaagcaga gaaggtggtg
    28501 agtgtataat tgtttgagtt aatgacattt gaaaatataa ttaaaagaat gttagatagt
    28561 gtaccagata cttttgataa aagggaaggt tctataatat ataatgctct tgctcctgtt
    28621 gctatagaac ttacagaaac atacattgca atggatgaat tactagacca aacattcgta
    28681 gatactgcta gttattacta tttagagaag agatgtaaag agagaggtat tacaccttta
    28741 cctgcaacca atacaattgc aaaaggagtt tttaatatag atattcctat tgattcaaga
    28801 ttcaacttag gagaatacaa ttatgtggca attgagagaa tatctgaaaa aacatataaa
    28861 atgaaatgtg aaactactgg acctgtattt gaacttggtc agttgattcc aattgaatat
    28921 gtagacaaat tagaaactgc tgagttaact gaaatactaa ttaatggaga agatgaagaa
    28981 tcagaggaca gtttaaggca acgatattat gatagtctaa attctcaatc ttttgggggg
    29041 aatatacaaa actataagga tgaagttaat aaattgcctg acgttggggg agttaaagta
    29101 tatccagttt ggaacggtgg aggtactgtt aagttagtaa taattaattc aaattttaaa
    29161 gtaccaagta ctgatttagt taatttagtt caagaagaaa ttgacccaaa tatgaaagga
    29221 gaaggtttag gattagcacc aataggtcat agagttacag ttgaaggagt tacaagtaca
    29281 actataaata tatcagcaga aataacatat aagagtggat atacatggga gaatataaaa
    29341 tcaattgcag aagaagcaat agacgactat ttaaatgagt taaatatgag ttgggaagat
    29401 gaagaaaact taatagtccg tatatctcag atagaaactc gtttgttaag tatagatgga
    29461 gtattagata ttgcaaatac gatgataaat agtattgcat ctaatctaac aatagatagt
    29521 aacagcatag tagtgagagg tgaggtagtt ggataaagag attaatctaa taaattactt
    29581 accacagatt ttacaggata aagaagaata tataaaagta tttaatgcag aaaacaaaga
    29641 aataaaaaca ctacatgata aattaaagga cctatcaaat gaccagtttt tagaggacct
    29701 aactataagt ggtataaaaa gatgggaaaa gataatgtct ataactccta aaagtaatga
    29761 gagtttagaa gatagaaggt ttaggatttt tagtaaatat ataagtaaac taccttactc
    29821 agagagattt ttaaggaact ggctagataa tgtagttgga gaaggcaatt atgaattaac
    29881 tattaataat gctacttata acatacacct tgagagtgat gctagaaatc aagattggtt
    29941 tgaggaagtt cattcttttg taagtaatat taagccatgc aacatgacac tagattacac
    30001 tagagtttta gttagcaaag ataattatat gaattttgga gcaactacta ttacagggca
    30061 tgaggttaca atttacccat ggtcacctag tgatattgaa acacaaggag agattaatat
    30121 actcagtggc aatgggtttg gatatcaaga ggttacaatt tattagatag gaggtggtag
    30181 tttggctaca gataaaagtt actacacaat attaacagac ataggaaaag cgaagattgc
    30241 aaatgcaagc ttagtaggag agaaagttga ttttgttaag attcaacttg gagatggtgg
    30301 aggaaatgag tataatccta cagaagaaca aacagcatta aaaaatatag tttgggaagg
    30361 taaagtaggg aatgtcaaga ctgatgaaag catgacaaat tgcttaattt tagaaagttt
    30421 aatacctgct agtgctggtg ggtttgtagt tagagagata ggttatttag ataccgaagg
    30481 taatttacta gctatatcaa agtataggtc agcatataaa cctaaagtgg aagatggagc
    30541 agtaattgac atgaaagtaa aaactatctt tgttgtatct aatgtaaata atatagaact
    30601 aaagatagat ccaactataa tctttgccac attaaaagat ttacaggacc tagacactaa
    30661 aataggtact gttaatacta aaatagatac aactaaggca gaattaacaa gcaacataga
    30721 aactgctaaa acagagttaa atactagaat tgacacagaa aatgagaaac aaaatattaa
    30781 aattgaccaa ttaattgcag gtggttcaaa tgttgcatac actcaaaggg tcgcaattga
    30841 tgattgggtg gaggatgcag aaaatggatt caaagcaact gtaacacata gtttattaac
    30901 acagagaata gttgtaaata ttatagatgt tactacaaaa gaaaatgtag ttacaaactt
    30961 taaaattata gatgataatt ctatagaaat tagaagtgaa gtaaaagctg agttaaacgt
    31021 ttatgtgata aatggaaatg cagaaactca ttttattaat gcgactgtgg atgataacag
    31081 agtgtctgaa atgactactt attcgtctaa gaaaatcgag gatagattga ttaatataga
    31141 agaaaaagta aatggtggtt tatctagtat tgcaacaagt gtaaatgagt tgataactta
    31201 ttgttagaga ggagagtgag aaaatgcaga cagaatggaa ctttgggtac aatggttcgc
    31261 cacaaagtgt tatattgaaa cctggcaaat ataaatttga atgctggggt tcttctggag
    31321 gtatcaacaa ttcttcttgg catactgatg ctaaaggcgg atattctaaa ggtgaaatta
    31381 cattaaaaaa acaaactaca ttatatgttt acgtcggcga aagtggtttt gcttcttcat
    31441 ctacgagtaa taacactaaa agtggtttta atggcggtgg taaaggttat ttaaatcaac
    31501 aggttatggg tacttattat tctatgtacg gtggtggtgc taccgatata agacttgttg
    31561 gtggtgcttg ggatgatgag caaggtttgc tatctcgtat aattgtcgca ggtggtggcg
    31621 gtggttcata ttctccttat actggtggtg caggaggagg attag
//
LOCUS       DAGSWI010000038        31135 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.038, whole genome shotgun sequence.
ACCESSION   DAGSWI010000038 DAGSWI010000000
VERSION     DAGSWI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.038"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            98..607
                     /gene="ruvC"
                     /locus_tag="MJF80_001577"
     CDS             98..607
                     /gene="ruvC"
                     /locus_tag="MJF80_001577"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBZ0280246.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            600..1202
                     /gene="ruvA"
                     /locus_tag="MJF80_001578"
     CDS             600..1202
                     /gene="ruvA"
                     /locus_tag="MJF80_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBZ0280247.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            1309..2328
                     /gene="ruvB"
                     /locus_tag="MJF80_001579"
     CDS             1309..2328
                     /gene="ruvB"
                     /locus_tag="MJF80_001579"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBZ0280248.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            2646..3671
                     /gene="queA"
                     /locus_tag="MJF80_001580"
     CDS             2646..3671
                     /gene="queA"
                     /locus_tag="MJF80_001580"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBZ0280249.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            3683..4168
                     /locus_tag="MJF80_001581"
     CDS             3683..4168
                     /locus_tag="MJF80_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280250.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            4247..5368
                     /gene="tgt"
                     /locus_tag="MJF80_001582"
     CDS             4247..5368
                     /gene="tgt"
                     /locus_tag="MJF80_001582"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBZ0280251.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            5502..5795
                     /gene="yajC"
                     /locus_tag="MJF80_001583"
     CDS             5502..5795
                     /gene="yajC"
                     /locus_tag="MJF80_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBZ0280252.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            5853..6215
                     /locus_tag="MJF80_001584"
     CDS             5853..6215
                     /locus_tag="MJF80_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBZ0280253.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGFLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            6281..6424
                     /gene="scfA"
                     /locus_tag="MJF80_001585"
     CDS             6281..6424
                     /gene="scfA"
                     /locus_tag="MJF80_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBZ0280254.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            7050..8945
                     /locus_tag="MJF80_001586"
     CDS             7050..8945
                     /locus_tag="MJF80_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBZ0280255.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            9408..10811
                     /locus_tag="MJF80_001587"
     CDS             9408..10811
                     /locus_tag="MJF80_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBZ0280256.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     regulatory      10983..11068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            11192..17134
                     /locus_tag="MJF80_001588"
     CDS             11192..17134
                     /locus_tag="MJF80_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HBZ0280257.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGAGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKSEFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDKICEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENIEQIDNNSNNKDENSDKQE
                     ANSNTNQDKNNDKENSSMDKPDLGEDTPKKESNISKDDNSTTSTYSRSVLETYADVQN
                     SDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNLYKEIPKPEMNIPKINK
                     ISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTIEVRDIEA
                     YGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEFQVVPDTK
                     PEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIRGTSSKIE
                     GLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKENGGFGIPT
                     NHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRGPIITFDK
                     SYTMDTFMVTPRFDVSDKTINDFEVRYWDENGQLHNLGNVPFETKTDPVNNNKYVMVR
                     LKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVELKEDVTQE
                     KITELKDRLNTPDKASGEYHPYKTVIDRELKLAQDLYNDRATLTDVTTVNQEINKSGK
                     YKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYTQYYGESG
                     AYKSGEINLKTGKNVIQLSKLHSLDIECGGSLYIRYPEDTPSGGDIKVRVAGATKIPH
                     LNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSYGYSEKTG
                     VLNTTDIESDKVTLNVPATAIYQGISKGNADLNTQVDRFYNSMLAWEQIMDLVYSERG
                     VFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHVGVEYGSV
                     PGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLALLAQTFD
                     DKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGSMLKNNSD
                     VDLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFKAWGVEIT
                     PELNSIMDSKNYEKESRKIQYVNDEARRKTLNENIASMAKDTKVSASFSDGIQSGSLV
                     KSSKISIDLDVDKDKDKILGYEIIRSDGNYLEGENGTQVKYRTVGFVDAKGNSKAKFT
                     DNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITNTVSDGDV
                     RTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEENPYIIVD
                     MKETLPIVGLKYTKPKTETRKFSLKGLFNFRKDTSTTYNPLTNYKIQVSNDKKNWEDV
                     SQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIEVAELDII
                     GPPGDNIEIGTMNNADQTRTNGIGKLAEDYVYQSDDPSTSEDETAKIPAGSIVITGEY
                     SGNPAFNLPLLIDKNNKTISGESLLFAEVPEKGELGEISKGTWVYYMPKENFDSLSDK
                     VKAELYRYNDLQGDTPVGQRFVSDTMCVPVGAKNYDELKTISLTDSNSKARKATRNAI
                     DVSNKKVITVSNRVKVNIVRDK"
     gene            17345..19225
                     /locus_tag="MJF80_001589"
     CDS             17345..19225
                     /locus_tag="MJF80_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HBZ0280258.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTSDKPALK
                     IVTYNNKTVDYENINSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGVDRFETA
                     VKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRISGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            19592..21193
                     /locus_tag="MJF80_001590"
     CDS             19592..21193
                     /locus_tag="MJF80_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBZ0280259.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNHLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASKSNGQPPTITVGSTEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGIL"
     gene            21412..23010
                     /locus_tag="MJF80_001591"
     CDS             21412..23010
                     /locus_tag="MJF80_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBZ0280260.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNNLKPNIEGTVDTSKAGTYVLNISAFDNFGQKCEINVNVKVIINTSTKDPNS
                     YEFKAMVSNEIYNLVNSYRDEKGKKPLKELKSLTGMANAWSKYMYEKEIFAHEINGKN
                     AAEVFSGFGMRSGENIAYLPMNTQSIYGDKEAKEIANSIFDLWKKSSKYNDNLLKSEF
                     YSFGFGMYVSSNSEVHATMEFLNS"
     gene            23245..25077
                     /gene="slpA"
                     /locus_tag="MJF80_001592"
     CDS             23245..25077
                     /gene="slpA"
                     /locus_tag="MJF80_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBZ0280261.1"
                     /translation="MKKRNLAMAMAAVTVVGSAAPVFAAASDVISLQDGTNDKYTVSN
                     TKASDLVKDILAAQNLTTGAVILNKDTKVTFYDANEKDSSTPTGDKKVYSEQTLTTAN
                     GNEDYVKTTLKNLDAGEYAIIDLTYNNAKTVEIKVVAASEKTVVVSSDAKNSAKDIAE
                     KYVFEDKDLENALKTINASDFSKTDSYYQVVLYPKGKRLQGFSTYRATNYNEGTAYGN
                     TPVILTLKSTSKSNLKTAVEELQKLNASYSNTTTLAGDDRIQTAIEISKEYYNNDGEK
                     SDHSADVKENVKNVVLVGANALVDGLVAAPLAAEKDAPLLLTSKDKLDSSVKSEIKRV
                     LDLKTSTEVTGKTVYIAGGVNSVSKEVVTELESMGLKVERFSGDDRYETSLKIADEIG
                     LDNDKAYVVGGTGLADAMSIASVASTKLDGNGVVDRTNGHATPIVVVDGKADKISDDL
                     DSFLGSADVDIIGGFASVSEKMEEAISDATGKGVTRVKGDDRQDTNSEVIKTYYANDT
                     EIAKAAVLDKDSGASSSDAGVFNFYVAKDGSTKEDQLVDALAVGAVAGYKLAPVVLAT
                     DSLSSDQSVAISKVVGEKYSKDLTQVGQGIANSVINKIKDLLDM"
     gene            25319..27661
                     /gene="secA"
                     /locus_tag="MJF80_001593"
     CDS             25319..27661
                     /gene="secA"
                     /locus_tag="MJF80_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBZ0280262.1"
                     /translation="MTVLDRIVDKSDELEIKVLNYIVDDIETLEPKMESLSDEELKDM
                     TNIFKDRLKQGETLDDILQEAFAVVREVSKRVLGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPIYLNALLGKGVHVVTVNEYLAQRDKETMQPVYEFLGLSVGVITSDLL
                     PEDRKKQYNCDITYGTNNEFGFDYLRDNMVTRKSEKVQRELYYSIVDEVDSILIDEAR
                     TPLIIAGSGKEDTKLYELANAFIKTLKDKDYEIDKKENTVCLTHTGLSKAESFFGIEN
                     ITNIDNIEIYHHTNQALRAHKLMERDVEYVVKDEEIQIVDEFTGRILDGRRYTDGLHQ
                     AIEAKEGVKIKDESKTLATVTFQNFFRLYEKLSGMTGTAKTEESEFESIYKLNVVQIP
                     TNKPVIRADLQDKVFRTEDAKYNAVVEEVIKINKTKQPVLVGTVSIEKSEMLSKLLKK
                     RGIKHEVLNAKQHDKEAEIISRAGKLGSVTIATNMAGRGTDIALGAGDKIEEKKVRDI
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDEMIKLYGGKGIEKILKR
                     MNAKDEDAIESKSLSKAIERAQRGIEGKNFEQRKNVLKYDDTINEQRKVIYEERNRVL
                     NDLNISEDIQKMVREIIEDAANKYLSNQRDYYGYFKSLYNDFMPPNTLLIPGVDKKSV
                     DEVVQHTYEISKRVYDIKKMEVGIDKIEELEKRVLLQVVDTYWMDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYALEGYDMFEELNRKIRTTTVQYLYKFD"
     gene            28272..30137
                     /locus_tag="MJF80_001594"
     CDS             28272..30137
                     /locus_tag="MJF80_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBZ0280263.1"
                     /translation="MNIPKKIISLLTITIMLSGLSLNTVNASTKESLVGSGRWETAIQ
                     ISKNGWENSKEAVLVNDSSIADALSATPFAKAKNAPILLTQSNSLDARTKAELKRLNV
                     SKVYLIGGNVTLSSNLEYELKSININSERISGDSRYSTSLELAKKIDSINTISKIVVV
                     NGQKGLADAVSVGAAAAQGNMPIILSDTTNGTSTSKDFISKHNITTSYVIGGTNSVSE
                     DIKNSLPNAKRISGENRNQTNAKVVEEFYKGTNLKNLYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVLLAGNKLDSVQKDILDTKIFDKITQVGGNGNEDVFKSIQDIQEETKYTV
                     ETISELDTAIKKSDANDIIRFKNDDKITGSIDISTKKAITIEFNGLYSGKVNINIPNG
                     DVYNYGDMTGQMDIKDIKTDTLVNEGSINEITIYDPNGCKIENEKDADIWKITLLDTS
                     KNTYINNSGTITKIVSDASNTTIKNSGKIELISGTKSPAVSGNQATDENIKDNDDKEA
                     GGISVYVDPCSPPKEGKVMLRIPKEPRSSSYNIYYRIVKSRPSAMDVGDTISKRGWTK
                     VTTRDSFMISATDGYYVEAVEIYDSDRKVSKWGRSGSIDDGYNNTTSSATLRSYK"
     gene            30446..30904
                     /locus_tag="MJF80_001595"
     CDS             30446..30904
                     /locus_tag="MJF80_001595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280264.1"
                     /translation="MNITNKCVLGISILAILSFGFSAFQGNAQITDRKKDYDIYLKAN
                     SDKSDVKSSLNDISKLEKKYGESDALSISKINLYEKMNDYKNLQSEIEKVYNRYPQTR
                     NTVSFVLLYSEVALKNGNKELSKNMINEAIKLGAQDAYKERVDKVLSQLN"
     gene            30917..>31135
                     /locus_tag="MJF80_001596"
     CDS             30917..>31135
                     /locus_tag="MJF80_001596"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280265.1"
                     /translation="MLDKYYNRGKVEQIDKFILFILLFMMCIIPIITHEYTSTNYSPI
                     FTLTLYSSGERVEIFNFYKTAILYLGTMI"
BASE COUNT        12838 a         3345 c         5784 g         9168 t
ORIGIN      
        1 tttgttaata ttatttatga tataatgatt tagaacataa gtttttatga gaaatacatt
       61 ttggtatttc ttttattttt agggggaatt ttaaaaaatg ataatattgg ggattgaccc
      121 aggtatagcc atagttggat atggtataat tgaatacaaa aatagcaagt ttaaagcaat
      181 cgattatgga gcagttacaa cacctgccca tatgaatata tcgagaagat tggaacttgt
      241 gtataaagga attgatacaa tagtaaagaa ttacaatata gatgaagttg gaatggaaga
      301 attattcttt aacaagaatg taaaaacagc tataacagtt gcacaagcta gaggtgttac
      361 tatgcttgca tgtgctcata atgggaagcc tgtatatgaa tacactccac ttcaagtaaa
      421 acaaggtgta gttggatatg ggagagcaga taaggcacaa gttcaacaga tggtaacttc
      481 atttttaagt ctaaaaaaag ttccaaaacc agatgatgtt gcagatgctt tagctgtggc
      541 tatttgccat gctcattcaa acaaacttga aaaaactcta aagaatatag gtggtaagta
      601 tgtatagtta tataaaaggt acagtagaag aaatttatat agacagtata gttgttgaaa
      661 ataatgggat aggatataaa ataaatgtat catccaatac aataatgaat ttacaagtgg
      721 gagaagccac aaaaatatat actaaattaa ttgtaagaga agatgatatg agtctttgtg
      781 gttttgtaag tagggaagaa cttaagatgt ttgaattact aacatctgta tctaaaatag
      841 gacctaaggt ggcgctttca atactatcat ttgcatcacc agcacagtta ggagcatata
      901 ttttaagtga agacattgga aaattatcca aagctcctgg tgttggtaaa aagacagcag
      961 aaagaatcgt cttagagctt aaggataagg tagacaaaaa taacatagag tttgaaccaa
     1021 cgttattatc acaaaaacct accttgatat cacaagatga gtctgtagat gcacttgtag
     1081 ctttaggcta tactttgtcg gaatcaaaag aggcagttca aaaatgtaaa aaagatggta
     1141 tgaatacaga agctattata aagaaagcac ttacatatat aatgagtaaa tccttgaaat
     1201 agtatttgga ttttataata tttggatttt aaaacaatat ttaaggagtt tatgtaaatt
     1261 tgaaaatata taaagtagat tttcaaaagt acgagaggag gggtataaat gcaaggtttt
     1321 gaagatgaaa ataggataat aacttctaca atgaagatgg aagatataga tatagaaaac
     1381 agtttaagac caaaaacttt agaagattat ttgggtcaag aaaaatccaa agaacaactt
     1441 agtatattta tagaagcagc taaatcgaga aatgagcagt tagaccatgt gcttttatat
     1501 ggaccaccag gtcttggaaa aactacacta gcaagtataa tagccaatga gatgggggta
     1561 aatttaagaa ttacgtcagg acctgcaata gaaagagctg gagatttggc agccatactt
     1621 acgaatttaa atgaaaatga tgttttattt atagatgaga ttcatagaat caatagaagt
     1681 gttgaagaag ttttatatcc agcaatggag gatttttgcc tagatataat aatagggaaa
     1741 gggccatctg ctagaagcat aagacttgac cttcctaaat ttacacttat tggagctact
     1801 acaagagctg gtatgcttac aaatccactt agagatagat ttggtgttat ttgtaaattg
     1861 gactattata cagttgatga actttcaaaa atagtactta ggtcttcaag catcttagat
     1921 gcagagatac agtctaatgg agcattagaa cttgcaaaac gttcaagagg gactccaagg
     1981 atagcaaata gattactaaa aagagttaga gattttgcac aagtcagagc tgatggaaaa
     2041 ataactgata aagtggcaaa agatgctctg gaacttttag gagtcgatag tttgggtctt
     2101 gattttgtag atgagaagct tcttatgact ataatagaaa aatttagagg aggaccagtg
     2161 ggtttagata cattggcagc ttctatcgga gaagatagaa atacaataga agatgtatat
     2221 gagccatatt tgcttcaatt aggatttata aatagaggtc ctagaggaag agtagccatg
     2281 ccattagcat atgaacactt aaaaattcca tatcctaatg aaaaataaaa atagattata
     2341 cgagtattta taaaataaaa gagtcattaa attaaaatat ttctaaaatg gctaaaaatt
     2401 caaacatatc cacaaaatgg taattatatc tgtagatact gtggataaac acgataaaaa
     2461 ttgtgtgtat tgtatctata tattgtggat aagacatgta tatcgtggaa aaatatctat
     2521 atattgtgga tatggtgtct gttaaaaata gcaatttctt gatagattat ggtaatattg
     2581 tggaaaataa gttgtttttt tgatattatt gtagctggag aaattttaag agaggaagtt
     2641 taacattgaa aacaagtgat tttaaatttg acttaccaca ggaacttata gctcaagttc
     2701 ctatagaaga tagagctagc tcaagattga tggtattaga taaagagaca ggtaatattg
     2761 aacataaagt atttagagat ataatagaat atttaaatcc aggagattgc ttagtattaa
     2821 ataatactag agttatacca gctagattaa taggagagaa gcttgaaact ggaggtaaaa
     2881 tagagttttt attattaaaa agaacagagg aagatacttg gcaggcttta gtaaaaccag
     2941 gaaaaagagc aaaagtaggg actaaatttt cttttggaaa tggaaagcta attggagaag
     3001 ttgtagattt atcagatgaa ggttcaagaa ttataaaatt ccattatgat ggaatttttg
     3061 aagaaatatt ggatgaactt ggaaatatgc cactacctcc atatatcact gctagactag
     3121 atgaaaaaga aagataccag actgtttatt caaagcataa tggttcagca gcagcaccta
     3181 cagcaggatt acattttaca gaagaattgt taaataaaat aaaagaaaaa ggtgtagata
     3241 tagcctttgt aactctacat gttggcttag gaacttttag accagtaaaa gtagaggatg
     3301 ttttaaatca caagatgcat tcagaatatt atatggttag ccaagaagca gctgacaaaa
     3361 taaatagagc aaaggaaaat ggaaaaaatg taatatgtgt tggtactacc agttgtagaa
     3421 ctatagaatc agcttgtaat gaggatggaa agatgaaaga aactagtgga tggacagaaa
     3481 tatttatata tccaggttat aaatttaaag ttttagataa acttattact aattttcact
     3541 taccagagtc tacactaata atgttagtaa gtgctatttg tggaaaggat aatgttttaa
     3601 atgcatataa tgaggcagta aaagaaagat atagattctt cagttttgga gatgctatga
     3661 ttataaagta gggaggtaat ggatgcatag tttacaaaaa caaaaaaaga ataaccctat
     3721 attaaagatt gcttttatat ttgaacgtgt tttagcagta gtagttctaa tagcagtatt
     3781 tttaggaact atagatgttc taagacttat gtggggagca tatatagttg attttcaaaa
     3841 tccagtacaa tacagtcagc ttaatgattt tttagcacaa atattacttt tagtaatagg
     3901 tgttgaactt gtagtaatgc tatctcttca tataccagga gcatttattg aggcattact
     3961 ttacgcaatt gctagaaaaa tgttgctact gcctaaaaat gaaggtatga tagatgtttt
     4021 aataggagtt atagctatag caggactatt tgctataaaa aagtttttag taacaaaaga
     4081 caagagtgct cttaatataa tgagtattca tgatgaagaa gcaattaagg aacaacagaa
     4141 caaagaagac gaagataaat ctatttagac aataaattat atgaatagct tattacaaat
     4201 atatcttaag aaataatgag aaataaatat gattaaggag aactttatgt acgcagtaag
     4261 atacgaattg ataaagactt gtaagcagag tggtgcaagg ttaggaagat tacacactcc
     4321 acatgggatt attgaaacac caatattcat gccagttgga actcaagcaa ctgtaaaatc
     4381 tatgacacca gaagaattaa aagagattgg ttcacaaata atacttagta acacatacca
     4441 tttatatatg agaccaggtc atgaacttat aaaaagagct ggtggtcttc ataagtttat
     4501 gaattgggat aaaccaatac ttacagatag tggagggttc caggtattta gtctagggcc
     4561 tttaagaaag ataaaagaag aaggtgtaga atttaggtca cacttagatg gttcaaaaca
     4621 ttttctaact ccagaaaaag ctatggaaat acaaaatgca ttgggttcag acataatgat
     4681 ggcatttgat gagtgtgcac catacccatc agatagagaa tatgtaaaaa attctttaga
     4741 aagaacaaca agatggttaa aaagatgtaa agatgcccat aataataccg acaagcaagc
     4801 tttatttggt ataatacaag gtgggatgta taaagactta agggaacaat cagcgaaaga
     4861 aataactaat attgatttac caggatatgc tattggtgga cttagtgttg gagaaccaaa
     4921 accacttatg tatgacgtgt tagagcatac aactccactt atgccaaagg ataaaccaag
     4981 atacctaatg ggagttggta gtccagatga cttagtagaa ggtgttataa gaggagtaga
     5041 catgtttgat tgtgtcctac ctactcgtat cgctagaaat ggaactgcaa tgactagcca
     5101 aggtaaagta gtagttagaa atgcaactta tgctgaagac tttactccac ttgaccctga
     5161 atgtgattgt tatgcttgta aaaactattc aagagcttat ataagacacc ttataaaagc
     5221 aaatgaaata ctgggtgcaa gattaataac tactcataat cttcactttt tattaaattt
     5281 aatgaaacaa ataagacaag cgataatgga agatagatta cttgatttta gaaatgaatt
     5341 ttttgcaaaa tatggatatg aaatataggt tttgcactat taattataat cattttaata
     5401 tataatattc aatagtactc aaagatagag aaagtaaatt gttatataaa catttattat
     5461 gatttacaaa agagtataat ttacaaagga gtgaaaaatt aatgccacag caacaaataa
     5521 ttatgagtat aggtctatgg gtagtagtaa tcgcaatctt ttatttccta atgattagac
     5581 ctcaaaagaa aaaagacaaa caattaaaag aaatgagaag tagcctaagt gtaggagaca
     5641 aggtcattac aataggtgga atagtagcta atgtagctaa agttgaagat gatgttgtaa
     5701 tattagaatt aggaccaaat agaactaaag ttccatttga aaaatgggct ataggaactg
     5761 ttaaatctaa aaaagaagaa atagaagaag actaaattag taatttaaaa gccttttctc
     5821 gaatatatat taatttgaga ggaggttttt ttatgggaaa atctaattat atttttaaag
     5881 gtctaggata tgcatatata ataactttgg ctgttttatt agtgtataac ttatttttga
     5941 catttacaga tattggtgga gataatataa ctatggtctc atcatttata actactattt
     6001 ctgcagctat aggagggttt tacacttcaa aacacatgaa agaaaaagga cttatgtatg
     6061 ggtttttagt aggacttttg tacatagtat gtattttttt gactgtgttt ttagcacaag
     6121 aaaagtttgt atttgaagta gggatgattt ataagttatt gttaatatca gcagcaggag
     6181 gcattggagg cgtattgggt gtcaacttta aatagagttt atattaaagt agttttatga
     6241 tataatcaat aggaaacaat tagaaatgga ggcgttgaaa atggaaaata aacatgtaaa
     6301 aactttatct caagctactt taaagcaaag tgctgcaaaa ggtggatgtg gagagtgtca
     6361 aacttcttgt caatcagctt gtaaaacttc ttgtacagtt gcaaatcaag agtgtgaaag
     6421 atagaaaatt attgatttag ttgtttgtaa gagggtatct caaagtgatt cttttatcat
     6481 tatgaggtat cctcttttat tttcaattat tatataaaaa tatatattct atttatgtaa
     6541 aattaaattt gatttcttat cgtttaatta tgcaaaaata tctaatcata taagagcatt
     6601 tattttaaag tagctactct tatattttat tttttatcaa ttacatgtat tagaataatt
     6661 tttaatttta aactctaatt tatgatgatt tttataatat gttatatatt ttaatatatg
     6721 gagatagata ttaatttagt gatatatatt taagtaataa gagtaaagca aaaattagtg
     6781 catagtagta tataaaaatg gagtttgaat acttttgtta gtatatgatt gtttagtaga
     6841 aaatcactat aaaataggat ttctatcttt gagcataatt atagagagct aagtaggggg
     6901 aagggtttca agaagatatt attagtgttt gtgttagtaa attatgtaaa cacataaaat
     6961 atattgaaaa ataatattat atataatatt atttatgtgt gggaaatacg aatataaaaa
     7021 ataaaaaata atattcggag gacataaata tgttaagcaa caaaaagaga agtatggcaa
     7081 tagtaatggc aggtgctaca gttatgagtg cagcagcacc aatattcgca gacaatactg
     7141 tgacagaaaa tgttgataaa aattacacag taagtgcgaa agattctgct aaactaatcg
     7201 aagaggttag aaaagcatta gaagttaagt ttgaagatac aaaagcagga gcaaatgtaa
     7261 atgatagagt ttacgatata aaagtagaca atgtaaactt aactaatgca actcaattac
     7321 aaaataaaat aaactcttta acagaagggc aaagtttaaa agttactatt caagataaag
     7381 ggcatcaagt gttaggtgga aaagtagttg actataaaat tgaaaactac aaaactgctc
     7441 aagaaatagt agatgcagtt aatgcttaca atgcaacttt agcagaagat tctgataaca
     7501 agttaacagc aactataaaa tctacaaata cagttgaagt aaaaagagca aaagattcag
     7561 caaatgttat tacattaaat gtaggagacc aacatttaga tttctcaaaa gtgataacat
     7621 cagaagaagg tacttttgaa ggttatgaaa agagttatag tgacatagat tcaaaagaat
     7681 tacatacgat aacagttaaa aacgcagatt tacaagacat atcagcagaa gaattatttg
     7741 atggaattag attaactaca ttaggaagag aaatagttaa taaagttaaa aatggatatg
     7801 ctttaacatt tgaaaatgaa gcaatcctta ctcaagaaca agaagatagt gatgataaag
     7861 ataaaccaga aaaatcaagc tttgatatag ttttaagcaa ggcaaatgaa aagccagaaa
     7921 caataagtgt ttcaagtaaa aaccataagt tagttagaga cttacacaaa gtattaactg
     7981 atgtaaaaga tggaaaagaa ttaaaagtag aagttttatc tggtgattca agatttacaa
     8041 cagcagtaga agttagtaaa gagagattca aagatggaga agcagaagct ataattttag
     8101 ttggagaaga tgctatagtt gatggattag catcagcacc acttgcctct caaaaaaatg
     8161 caccaatatt attatctaaa aaagattcac taccatcaga aatagaagct gaaatattaa
     8221 gagtacttgg aagtaatcta tcttctaaga aaatatatat agtaggtgga gaatctaaag
     8281 tatcaaaaga aactgaagaa aaactttcta aattaggtgt aagtaaagtt gagagagttt
     8341 ctggagaaga tagatttgaa acttctttag aaatagcaaa acaattaaaa gatacattta
     8401 agactgcttt tgtagtaggt ggaaatggag aagctgatgc tatgagtata tcagctagag
     8461 ctgctcaatt tggtgctcca ataatagtta caggtaacga attagatgca aatgctgaaa
     8521 aattattaaa aggaaaagaa ttagaaatag tgggtggaga aaattctgta tcaaaagaag
     8581 ttgaagacaa attagttgat atagatttaa ataataaagt tgaaagatta gctggagaaa
     8641 atagaaaaga tactaatgct aaagtaatca ataaatacta tgcaggtgca actaaagcat
     8701 atgtagcaaa agatggttat gtaggtggaa atggacaatt agttgatgca cttacagcag
     8761 caccacttgc agctagttca aaagctccaa tagtattaac tacagaagaa ctttctaaat
     8821 cacaagaaga agtagttgag ttaagactta agaatgctac taagttagta caaataggtg
     8881 aaggaatagc taaaaacgct attgaaaaaa tagcagaaaa aataaactta tttactaaga
     8941 actaataaga tataggaatt aatattaaaa gtttatttag ataatatatg attatacaat
     9001 gcgttaatat gtaagacata gtgtagatat tataaaatat acaaagaaca agataaaata
     9061 ataaaaataa ggtgtctcat ttagaattag attgagatac cttattttat tttttgatta
     9121 taaatgttga gaaattcata ttgcaaaaat tattataaaa agatacttct tataatgatt
     9181 aaacatatta ctaacataaa ttattttgaa cttataactt aattaaataa aataaattat
     9241 aatgttttga atttatagag aataaaaact ttaggtatat tgtttgagtt gtatggataa
     9301 ctatggtatc atatataaaa tatggtaaca aaattgtaac ttttgattaa aaataagtat
     9361 gagtactaat aaaaaatatg aaaagtacat tgggggtaga aaataatatg aagaatagta
     9421 aaaagatatt agctatagga cttacactat ttttagtaat ggtaaatact cctatggtaa
     9481 atgcgttgac atcagttgag caaataaaag ggaatgacag atatgagaca gcagcaaaaa
     9541 tagcagataa gcaaaactac aatacagcaa tactaatcaa ctcagataat agcttagcag
     9601 atggtctaag tgcaagtggt ttagctggag ccttaaatgc gcctattttg atgacaaaac
     9661 aaaatcagat tccaaacaca actatggaaa gattaaacaa agcaaagact gtgtatataa
     9721 taggttcaga atcaacaata agtaaaaatg ttgagaatca gttactatcc aaaaagaagg
     9781 ttgtacaaag aatctttggt gaaaatagat ttgatacaag tataaaaata gctgaaaaaa
     9841 tcaaagaaat taagccaata gataaagtaa ttatagccaa tggatttaca ggagaagcag
     9901 atgcgataag tgcatcacca gtagctgcta gagatggagt acctataata cttacagatg
     9961 gaaatagtgt gggatttgac acgacaggct taaagagtta tgcgcttggt tctagtgaaa
    10021 taataagcga cgaattagtt aagagcacaa attctattag attgggtggc acagatagat
    10081 ttgaaactaa taagatagtt atacaagagt tttataaaaa tagtaaagaa ttttatctaa
    10141 gtaaaggctt acaattaaca gacgctttgg ctgcctctac aatagcaaaa aatgctcctg
    10201 tagttttagt tgaaaatggc agtaataaga gtattttaag tggtgcagac aaattgactg
    10261 tactaggagg cataaatcag aatgttatca agcaatgtat aaatcaagct tcaccaaatc
    10321 aacaaggttt atattacaat ccaaatgata gagcatttaa agagagaata aagggtaaag
    10381 tatatgctct tacaaagcag tatagaaaag agaatggagt aagagcgtta tctgtagcaa
    10441 gcagattaga gggtctagca aatgattggt ctaatttaat ggcaaataag aagactttat
    10501 ctcatacgat aaatggaaaa aattcatatt ctactttttt gaaatactta gattggagtg
    10561 agattaaacc tggatatata gcagttcaag gtgaaaatat aattaaatat aaaattccag
    10621 ataaacctgt atatacaaat agagatgcag atgatatagg aaactttatt tttaatgagt
    10681 ggaagacaaa tccagaagaa ggaactaaca tgttgcataa agggtatgaa ataatgggat
    10741 ttggtatagc tataacagga gataagaatc tatatgctac acatgaattt tatggaagat
    10801 ataaagaata agaaaatata cacaaaatta tgcttgtaag gaagctatat aaataattaa
    10861 attataatgg aaaataacat ttatattaat aaattaatat aaatgttatt tttttataat
    10921 ttaatctaaa aaaattgtat taaaaagttt aaatatagta taaaatgggt aagaaagtag
    10981 tgaaaattaa atggcaaaat tagggaaatc taatgacgca aaactatagg gattaaagct
    11041 tttaaagcca tatcagccag ttgctaaaaa gagtaaagtc tttttatttt ataattaaga
    11101 ttctctttga gtgtcttttt ttattgtctg aaacttatag tatttaaatt catgaaaaat
    11161 tagatattta ttaagggagg attgttattt catgaaaaaa gcaatatctt gtgtactagc
    11221 agtatctatg tgtagtgcac cactaaacgt ttttgcagaa cctatattag aggggaagct
    11281 aagggcagtt gaaaaatcag ttacagaaaa gttaaggggg agtcttgaag tagatttaaa
    11341 tttttcactg ccaataaaaa atagcgagtc agaaagtatg actaatatgc agttacgatt
    11401 aaaagatgat agtaacaata gtggaataat aaagcttgga gaaaaagatt caggaacagt
    11461 aaatgttggg acagataata tagaatatac tataaaaaaa ttatctgcaa gtagaagtga
    11521 aataaaaaat aaagatgaga atatatcata ttataatatt gtatttaata atttacctgt
    11581 tggaaaatac aatgtagaag tctctggagc tggatttaag aataaagaaa ttaaagacat
    11641 tgatatatca agttattcac aaagagtctt attaagcaat agagcaagtg tagataataa
    11701 aaataaacct ttaaattatg aattattctt aatgggtgat gtaaatggag acggtaaagt
    11761 agataagagt gattataata aggttttaga gaatatagat tcaaataaaa gtgaatttga
    11821 tttaaataga gatggaaagg tagatattgt tgacttagat tatgtacaga aaaatttagg
    11881 acaaagtgaa gatgcaaaaa gcctagaaag tatagtatct actaatccta tagtagatac
    11941 aagtaaggtt gaattaaaag gaaatagtga tgtagatatt aatggtaatg tagaaaattt
    12001 atttagtgga gaaaataatg gagttacaat atcttcaaaa aatgataaaa tatgtgagga
    12061 aaaaccagca gtaatggata tagctttatc agaagcactt aatatggaag tcatcaatat
    12121 aaaagctcca gaaggtactg ctccagcaaa aggaatagtt gaagttactg acaaagatgg
    12181 taatgttcaa cagattcctt atgaaaacaa agaatatatg aaaaacttaa aagatgagga
    12241 gcaacaagag gaagtaaata acgaacaaga agaagtaaat caagaagaag taaataataa
    12301 agatgaaaat atagaacaga ttgataataa ctcaaataat aaagatgaaa atagcgataa
    12361 acaagaagca aactctaata ccaatcaaga taaaaataat gataaagaaa attcatcaat
    12421 ggataaacct gatttgggtg aagatactcc aaaaaaagag agtaatatca gtaaagatga
    12481 taactctaca acatctacat atagtaggtc agtattagaa acatatgcag atgttcaaaa
    12541 tagcgatata gtaataaatc ttggtaaaca agtagcagta aaaaagataa ctataaaaat
    12601 aacagctaca acagcaaacg atagaaatct tgcagaaata tcaaaagttg aatttttaaa
    12661 caatttatat aaagaaatac caaagccaga gatgaatata cctaaaataa ataaaatatc
    12721 aactagcact gctgttggaa atgaaaaagt atctttttat tggaataatg aagtaaatgt
    12781 atcagcatac gaacttatac tagataaaat tgatagtaag ggaaaagtat tatctactaa
    12841 aaaattacaa actagtaaaa atacaataga agttagagat atagaagcat atggactata
    12901 tagagttagc atacaatctt taaatggaga ttggtctagt ggttataaag atgcaacacc
    12961 agaaaactta gatggagtac cagaaaatgt agattcaaac tataaaccta tacaatttag
    13021 cccagatagt atattagaat ttcaagttgt tccagacaca aaaccagaac caccagaagg
    13081 tataaatgtt aaaggtaaat ttaaatctct tgatgtaagc tggaaaaatc ataaacaagc
    13141 taaagacttt gacttgtact ataaagaaaa aggctctaat gggtcttgga taaaatacaa
    13201 tgataagctt attcgtggga caagttcaaa gatagaaggt cttaaagata atacaacata
    13261 tgaagttaga atgacagcta ctaaccactt gggaactagt ggaatgtcta aaacatatat
    13321 aggtacaact gaaagcatgg atgcaccaac aacacctaac tatagattaa ttaatactcc
    13381 taaagagaat ggtggatttg gaataccaac aaatcacatt gtagatgtta cttatccagc
    13441 aggatatgac aaaagtgaat atagtgataa aaatccattt gataaattca atgtagtaga
    13501 tggggacttt acaacacatt ggacattccc aacatataat gcagctgagg gaacgaatag
    13561 aggaccaata ataacttttg ataaatctta tactatggat acttttatgg ttacacctcg
    13621 ttttgatgtt tctgacaaga caataaatga ctttgaggta cgatattggg atgaaaatgg
    13681 tcaattacat aatctgggga atgttccttt tgaaacaaag acagaccctg taaataataa
    13741 taagtatgta atggttagat taaaagaacc aataactgct accaagatac aagtaagtgt
    13801 ttgttcatcc tattcatata agtctaatat atcagaactt aagttttatg agtatgacag
    13861 tatagaagat gatgtaagaa atctatttat ggatgaccta caagttgaat taaaagaaga
    13921 tgtaactcaa gaaaaaataa ctgaattaaa agatagatta aatactccag ataaagctag
    13981 tggagaatat catccatata agacagttat tgatagagaa ttaaaattag ctcaagactt
    14041 atataatgat agagccacac ttactgatgt aacaactgta aatcaagaaa taaataaatc
    14101 aggaaaatat aaagataaaa acggacagta tactgtaaat tccaataatt taggtatgca
    14161 aaatgactgg caggcattag gagtagctgc tagagcagga gatgaaatca cagtatatgt
    14221 tggaagtaaa agtggaaaaa ctcctaagtt gatatataca cagtactatg gagaatctgg
    14281 tgcatataag agtggagaaa taaatcttaa aactggaaaa aatgtaattc aattaagcaa
    14341 acttcatagt ttagatatag aatgtggagg aagtttatac ataagatacc ctgaagatac
    14401 tccaagtgga ggagatataa aagtacgtgt agcaggagct actaagatac cacatcttaa
    14461 tctaaatgga acaataaatg ataagagtcc tgcgggagtt tctgaatcta agaaaaagat
    14521 aaaagcatat atagaagaat tagctaaata taaagatgct gtaaaaggag aaccatatta
    14581 tccaggaaca aatgttggta attcatatgg atattcagaa aaaactggtg tgttaaacac
    14641 tacagatatt gaaagtgata aagtaacatt aaatgttcct gcaacagcta tatatcaagg
    14701 aatatcaaaa ggaaatgctg atttaaatac acaagtagat agattctata atagtatgct
    14761 tgcatgggaa caaataatgg atttagttta ctcagaaaga ggagtattta aaacacaaga
    14821 tttagatggt aatggaactg tagatgataa tgaatttaac ttaactaaaa atgatatggc
    14881 tccaaaatct cgtatgaaca taaaatacca aagaatgttt attggagcat ttatgtatgc
    14941 ttctggatta catgtaggag tagaatacgg ttcagttcca ggacttatga atggagtacc
    15001 tttccaaata gatgatagtg gaaaagctac aggaggaaat ttatttggat ggggaatagg
    15061 tcatgaaata ggacatgtta cagatattgg aaagatgaca tatagcgaaa ctagtaacaa
    15121 tgtacttgct ttacttgccc agacatttga tgataagaca cattcaagac ttgaaggttc
    15181 aacaatggat aagatatatg aacatgtaac ttcaaatagc ttaggtatac caagcaatgt
    15241 atttgaaagg cttggaatgt tatggcaatt acatctagct tatgatgatg attttacggg
    15301 ttcaatgtta aagaataact cagatgttga tttaagtaat gacacattct atgcaaagat
    15361 ttcaagaaaa tacagagcac tttcaagcag tgacccaatt aatagtttac caaaagacca
    15421 aatgctagta gcaatggcat caagtgttgt tgaaaaagat ttaagagatt actttaaggc
    15481 atggggagta gaaattacac cagaattaaa cagtataatg gatagtaaga actacgaaaa
    15541 agaatcaaga aagatacaat atgtaaatga cgaagctaga agaaagactt taaatgaaaa
    15601 tatagctagt atggcaaaag atactaaagt aagtgctagt ttttctgatg gaatacaaag
    15661 tggttctcta gttaaatcta gtaaaatatc tatagaccta gatgtagata aggataaaga
    15721 taagatatta ggatacgaaa taataagaag tgatggaaat tatttagagg gtgaaaatgg
    15781 aacacaggta aaatacagaa cagttggatt tgtggatgca aaaggaaata gtaaggctaa
    15841 atttactgat aatatatctc ctcttaataa cagagcattt acatataagg tagttgcata
    15901 tgattatcac ctaaatccaa cagaagaatt tgaagttggt actgtaaaat tatcagatga
    15961 agggaaaata aataaatcag catggtcatt cataacaaat actgtttctg atggtgatgt
    16021 tagaactgaa aatgactcac atgggccatt acaaaatcct gaaattgata atataaaaga
    16081 taatgatgca agtactacat ataaaggaaa gataataagt aaagcagagt ggaacaaaga
    16141 ccctcaaaaa gaccctgata taaatgtaga agaaaatcca tatataatag tagacatgaa
    16201 agaaacatta cctatagtag gactaaaata tacaaaacca aaaacagaaa ctagaaagtt
    16261 ctcattaaaa ggattattta actttagaaa ggatactagc acaacataca atcctttaac
    16321 taattacaag atacaagtaa gtaatgataa aaagaactgg gaagatgtaa gtcaaggaac
    16381 ttttgaatat ggacaagatt tagttggtgg aggaaaagac caagataatg aagctagagt
    16441 tacatttaat aaagatggta aactttggac atatcaagct agatatgtaa aactaatctc
    16501 aaataataag tctaatatag aagttgcaga attagatata ataggacctc caggtgataa
    16561 tatagaaatt ggcacaatga ataatgcaga ccagactcgt actaatggaa ttggaaagtt
    16621 agcagaagat tatgtatatc aaagtgatga ccctagcaca agtgaagatg aaacagctaa
    16681 gattccagca ggttcaatag ttattacagg tgaatacagt ggaaatccag catttaattt
    16741 accactactt atagataaaa acaataagac tatatctgga gaatcgcttt tatttgcaga
    16801 agttccagaa aaaggtgaat taggtgaaat cagtaaaggt acatgggttt actatatgcc
    16861 aaaagaaaac tttgattctt taagtgataa ggttaaggct gaactatata gatacaatga
    16921 ccttcaagga gatactccag taggtcaaag atttgtaagt gatacaatgt gtgtacctgt
    16981 aggagctaag aattatgatg agttaaagac aataagtctt actgatagta atagtaaagc
    17041 tagaaaagct acaagaaatg ctattgatgt tagtaataag aaggttataa ctgtaagcaa
    17101 tagagtaaag gttaatattg ttagagataa atagtaggtg gaactctaaa aaacgtaaaa
    17161 atactaatac taacattgta tatctaatat gattaggtat tagaatagtt ttaaaatagt
    17221 agatattaaa agtaaagtat ataaatgtaa ctataagttt agggtaattt ataaattaaa
    17281 tataaaagct aggtggataa gtaaatttat ccacttagtt aatgatagat aaggacgggt
    17341 gttaatgagt aaaagaaaat cttttataag aactatagca gtatcaacta tggcagtagc
    17401 tgtaactggt agtgctacat gtgcatatgc agctccagtt ttacaaggaa caaaaacgta
    17461 tgagaaagta aatactatag atatatctgt ggactcagta gaaaacatag tatattcatt
    17521 tcaagccagt ataaaagtac aaggtgaggt tgaagtagta gataatgagc agaaagaaaa
    17581 gattacttgg tctgataata taaaatctca gataaaaagt ggaaatgcaa atgctacttg
    17641 tagagcagaa tacaacaaat caagtaatac aactacatta gatatatatg ttacatcaaa
    17701 tgaagattta ttagacggaa atagattgaa tataggaaga atatctgtaa aaaaatctgg
    17761 tagtaactca aatgctgact ataaagtttt aggaaaagga acaagcgata aaccagcttt
    17821 aaaaattgta acatataaca acaaaacagt ggattatgaa aatataaact ctgatgaagg
    17881 attaatattt acccttatta atgagagtga agtaaaacca attggtggaa ctggttctag
    17941 taaaaatgac cctgaaaagt ataaggtaga gaaaagtgaa gcacttgaat atttattaaa
    18001 caatattagg ataaactatt ctattgttag taaagaaaca caagaaagtg gttcaaatgt
    18061 aatattaaaa cttggattag cacaaaaaac aactaaggga agaaaagcta caataaataa
    18121 atatgttgaa gttacacttc caaaaagttt agaatacata gttgaaaatg aattatccaa
    18181 accagatgaa ttaccaccag ataatggcaa tggtggaaat aatggtggag gaagtaattc
    18241 aggcggttcg tctagtggtg gaagctctgg aggaggaaat tcaagtgatt ctacatctaa
    18301 tgtcacagtc aaaaaattaa aaggtgttga tagatttgag acagctgtca aaatatctca
    18361 aagtggttgg acaaaatcag atacagttgt aatagtaaat ggagaagata aaagtatggt
    18421 ggatgggctt acagctacac cgcttgctag tgtaaaaaac tcaccgattc ttttatcatc
    18481 aaatgaaaag cttcctcaaa aaactgtaga agaattgaaa aggctaaatc catcaaaagt
    18541 aattgtgata ggtggaaaca actcaatgcc aaattcagtt gttgaagcta tcaaagctgt
    18601 aaattcaaaa atatcagtac aaagaataag tggagataca agatatcaaa cctctattaa
    18661 tatagcaaaa gaaatagata agacgaacaa tgtaagcaag ttgtatatag gagcaggaaa
    18721 tggagaagct gattcattat caatagcatc actggctggt aaagaaaaaa ctccaatagt
    18781 acttacacaa aaagatggtg tagataatga agctgaacaa tttattaaat caaataaggt
    18841 gtcaaatata tattttattg gtggagtaga aaaaatatct aacaaggcta ttgaacaagt
    18901 tggaaaaata gcaaacaaag atatatcaaa caatagggtg gcaggtcaaa ctagacaaga
    18961 gacgaatgcc aaagtaatag ataaattcta ctctcaatct aagctagatg gagttgtagt
    19021 tgcaaatcaa gacaaactta tagacgcttt agcagttgga ccattagcag ctaaaaacaa
    19081 ttcaccagtt atacttgcta caaatacgtt agataaatca caagaatcgt cattaaaagg
    19141 taaaaattca tcaaagttgt ttgaagtagg tggaggaata gcctcatctg taatagataa
    19201 gattaagagt ttaattgaaa aataaatatt aagtaaatag aaaataagaa tgtatttaga
    19261 tataaagtta tatatttaaa tgcattctta ttttattttc attatcttaa caatttaata
    19321 atattgaggt taaaatgtat aataatttga gctatattaa gtttaatttt aaataaagca
    19381 gatatctttt atatatataa atataaattc aattttgaat atgtttaaaa agaagttaaa
    19441 aataattttt taaatttaat aaaataaaac actaatatat agacgagata tattcatatc
    19501 atatatactc tataatatat ggaaaataaa gagaaaataa atggaaaata atcacaatat
    19561 aaatataaaa tataaaaatc atcaggggga tatgaaaatg aacaaaaaaa tattatcatt
    19621 aggtctagca gtatcattaa tcttagtaaa cttcaaaagt gtaaatgcat catcagtagt
    19681 agaaaaaata tacggtaaag atagatatga aacagcagca aagatagctg ataaacagac
    19741 ttatgaaaca gtaattttag taaatacaga aaaatcactt gcagatggat taagtgcaag
    19801 tggattatcg ggagctacaa aagctccaat actatttaca caacaaaata agataccagc
    19861 agacacaaat agatgtctaa aaaatatcaa aaaagcatat ataataggta cagaagatac
    19921 tataagtaaa tcagtagaaa aagaactaga ttctaaaaat atagaagtca aaagaattgg
    19981 tggagaagat agactaaaaa caagctattt aatagctaaa gaaatagcaa ctataaaaaa
    20041 agtggataaa gtactattaa ctaatgcata tagtggagaa gcagacgcaa tgagtgtatc
    20101 atcagtagct actagagatg gagctccaat catacttaca gatggaaaga gtgtgccttt
    20161 tgatgtaaaa aatattcaat catattgtat aggttcagag gagataatga gtaatcatct
    20221 agttaaaaat acaaactcag taagaataga aggaactgac cgatttgaaa ctaacaaaaa
    20281 cgtaatagac tactttttta atagtgcaga tggattctat gtatcagatg gataccaatt
    20341 agtagatgca atagcagctg caccacttac taaaaactct ccaatggtac tagtaaatga
    20401 tggaagcgat aaaactgtat tagaaggagc taagaatata acctctgtag gtgaaataaa
    20461 tgagaaggta atacaacagt gtataaatgc ttcaaagtca aatggacaac caccaacaat
    20521 tacagttgga agtacagaga tatataaggg tgaaaagttt gacactggca agttaaatat
    20581 agtggctaaa gataacacag ggaaggtatt accaatagaa gttgatgggt ttatagatac
    20641 aaatagagta ggtacataca tattgacatt aaaagctaca gacgaatggg gaaaaagtac
    20701 aggaaaaaga gtagaaataa aagtgttgga tgacaaatct catgattaca acagcccaga
    20761 atttaaaaaa atggtatcta ctgaaatgta taacttaatc aattcctata gaaaagaaaa
    20821 aggcaaagag cctttggtag tatctagtaa gctagaaggt atggcaaatg catggtctaa
    20881 gtatatgatg gataaaaaag tatttgcaca ttatatagat ggaaaaaatg ctccacaagt
    20941 attttctgag tttggaatga gaagtgagga aaatatagca tatatttata tagattctaa
    21001 gaacgttcaa actacacaag atgcaaaaga cttggcaaag gctatatttg aagtgtggaa
    21061 gaagtctcca gagtacaatg caaatatgct tagtgatgag ttttatagta ctggatttgg
    21121 gctttatata ttatctgatg gtcaagtgca tgctactcag gagtttttaa atggcaatga
    21181 aggtatcttg taaaaaattc cataaatctt gtttttttgt taatttactg atattataaa
    21241 atgaaaatca catctaaagt actttttgta atctgttttg ttattgcttt acaggttatg
    21301 gtattttggg tgttttttgt tttagtagtt tataaatttt gagggaatca ctactgagga
    21361 tgtaatttat gattagctga attatataaa aaaattagta ggaggagttt gatgaaggta
    21421 aataagagag tattatcaat aggattgaca atatcattaa taatggcagg agcaccaaat
    21481 atcaatgcac tttcgagcat agaaaagata caagggaaag atagatatga gacttctgca
    21541 ttgatagcag ataaacaaat atatgataca gtcatattag taaataccga taattctata
    21601 gtagatggat taagtgcaag tggattatct ggagttgtaa aagctcctat aatgttggtg
    21661 caaagagata agataccaac agatgttgaa aaaagactaa aggatgttaa gaatgcttat
    21721 gttataggta cagaggatac aataggtaaa tctgtagaaa atcaacttaa gaataaaggg
    21781 attgaagtta aaagaattgg tggggaagat agaataaaga ctagctatct tatagctaag
    21841 gagatttcag ctattaaacc agtaaatgat ggtgataagg tttttcttgt aaatggatac
    21901 acaggtgagg ctgatgcaat gagtgtatct tctgtagctg ctagagatgg agttcctgtt
    21961 atacttactg atggaaagag tataccattt aaggttgatg gcgttcaatg ttattctctt
    22021 ggctcagagg agataatgag taatgaactt gtgagtaaga ctaattctgt taggattgct
    22081 ggtaaggata ggtttgagac taataagaag gttatacaac gcttttataa aggaacaaag
    22141 aagttttatg tttctcaagg gtataagtta gttgatgctg tggctggttc tccgttggct
    22201 aaggacaaac ctatagtact tgttaatgat ggaagtgata agagcgtgct tagaggtgct
    22261 gatgaagtta cttctttagg tggaatggat aagaaggttg ttgaccaatg tattagttct
    22321 gcatctgata aaaatacaat gcctacaatc actgctaatg atgtagaaat ttctgtaggg
    22381 gataagtttg ataatagtat gttgaatata gttgccactg attattatgg aaataatttg
    22441 aagcctaata ttgagggaac tgtggatacc agtaaggcag gaacgtatgt tttgaatata
    22501 agtgcttttg ataattttgg gcaaaagtgt gagattaatg taaatgtcaa agtaattata
    22561 aacacaagta caaaagaccc taatagttat gagtttaagg ctatggtatc taatgaaata
    22621 tacaatctag ttaattctta tagagatgaa aaaggtaaaa agcctttgaa agaattgaaa
    22681 tctttgactg gaatggcaaa tgcatggtct aagtacatgt atgaaaagga aatatttgct
    22741 catgaaatta atgggaaaaa tgcagctgag gtattttctg gttttggaat gagaagtggt
    22801 gaaaatattg catatctgcc tatgaatact caatctatat atggagacaa agaagcaaaa
    22861 gagattgcta attctatctt tgatttatgg aaaaagtctt caaaatataa tgataatcta
    22921 ttaaaaagtg aattttacag tttcggattt ggtatgtatg tatcatcaaa tagtgaagta
    22981 catgctacaa tggaattttt aaatagttaa tttaaaattg aaacgataat ttaaaaatga
    23041 aaataatttt aaaaaataaa attttaggga aaaaagaaga aaaaagttgg aaaaaacttc
    23101 taaaaatttt gtataactta ttgaaaattt attttagata gtctatcata gagatgtgag
    23161 aaatatttgg attaatatga acatgaaatt ttaatgtaca taataagaga tgtaatttaa
    23221 tataatgttg ggaggaattt ttaaatgaaa aaaagaaatt tagcaatggc tatggcagct
    23281 gttactgtag taggttctgc tgctccagtt tttgcagcag cttcagatgt aatatcacta
    23341 caagatggta caaatgataa gtatacagta tcaaatacta aagctagtga cttagtaaag
    23401 gatattttag cagcacaaaa cttaacaaca ggtgcagtta ttttgaacaa agatacaaaa
    23461 gttactttct atgatgcaaa tgagaaagat tcttcaactc caactggaga taaaaaagtt
    23521 tattcagaac aaactttaac tacagctaat ggaaatgaag attatgtaaa gacaacttta
    23581 aaaaatttag atgcaggaga atatgctatt atagatttaa cttataataa tgctaaaact
    23641 gttgaaatta aagtagtagc agctagtgaa aaaacagtag ttgtatctag tgatgcgaaa
    23701 aatagtgcaa aagatatagc tgaaaaatat gtgtttgaag acaaagactt agaaaatgca
    23761 ctaaaaacta taaatgcctc agatttcagt aaaactgata gttactatca agtagttctt
    23821 tatccaaaag gaaagagatt acaaggtttc tcaacttata gagctacaaa ttataatgaa
    23881 ggaactgcat atggtaatac accagtaata ttaactctaa aatctactag taagagtaat
    23941 ttaaagactg cagtagaaga gttacaaaaa ttgaatgcta gttattctaa tactacaact
    24001 ttagctggtg atgacagaat acaaacagct atagagataa gtaaagaata ttacaataat
    24061 gatggcgaga aatcagatca ttcagctgat gttaaagaga atgttaaaaa tgttgtatta
    24121 gtaggtgcaa atgcactagt agatggatta gttgcggctc ctttagcagc agaaaaagat
    24181 gctccactat tattaacttc aaaagataaa ttagattcgt cagtaaaatc tgaaataaag
    24241 agagttttag acttaaaaac ttcaacagaa gtaacaggaa aaacagttta tatagctggt
    24301 ggagttaata gtgtatctaa agaagttgta acagaattag aatcaatggg attaaaagtt
    24361 gaaagattct caggtgatga tagatatgaa acttctttaa aaatagcaga tgaaataggc
    24421 ttagataatg ataaggctta tgtagttggt ggaacaggat tagcagatgc catgagtata
    24481 gcttcagttg cttctactaa attagatggt aatggtgttg tagatagaac aaatggacat
    24541 gctactccaa tagttgttgt agatggaaaa gctgataaaa tatctgatga cttagatagt
    24601 ttcttaggaa gcgctgatgt agatataata ggtggatttg caagtgtatc tgaaaagatg
    24661 gaagaagcta tatcagatgc tactggtaaa ggcgttacaa gagttaaagg cgacgataga
    24721 caagacacta actctgaagt tataaaaaca tattatgcta atgatactga aatagctaaa
    24781 gctgcagttt tagataaaga ttcaggtgct tcaagtagtg atgcaggagt atttaatttc
    24841 tatgtagcta aagatggatc tacaaaagaa gatcaattag ttgatgcatt agcagtagga
    24901 gctgttgctg gatataaact tgctccagtt gtattagcta ctgattcttt atcttctgat
    24961 caatcggttg ctataagcaa agttgtagga gaaaaatatt ctaaagattt aacacaagtt
    25021 ggtcaaggaa tagctaattc agttataaac aaaataaaag atttattaga tatgtaataa
    25081 attttcttga taaaataaag aactccttat aaaggagttc ttttattgtg ttaaatattc
    25141 aaaaaagcaa aaaggagact agaatagtct ccttttattg tgagaaatac ttaaatataa
    25201 gagatataac tatttattta agtaaactta ttatactata cttaaaaaaa aatgtctact
    25261 tatccataat tttaatgtat aatagtattt gagaaacaaa taaggaggaa aactacaaat
    25321 gacagtttta gatagaattg tagataagtc agatgaatta gaaattaaag tacttaatta
    25381 tatagttgat gatatagaga ctttggaacc aaaaatggaa tctttaagtg atgaagaact
    25441 aaaagatatg acaaatatat ttaaagatag attaaaacaa ggtgaaactt tagatgacat
    25501 attgcaagaa gcttttgcag tggttcgaga agtttctaaa agagtacttg gaatgcgtca
    25561 atacagagtt caattaattg ggggtatagt actacatcaa ggaaaaatag cagaaatgag
    25621 aactggtgaa ggtaaaacac tagttggtgt tgctcctata tatttaaatg ctctcttagg
    25681 gaaaggcgtt catgtagtta ctgtcaacga atatcttgca caaagagata aagaaactat
    25741 gcaaccagtt tatgaattct tagggttaag tgtaggagtt attacgtctg atttattgcc
    25801 agaagataga aaaaaacaat ataattgtga tataacttat ggtacaaaca atgagtttgg
    25861 atttgattat ttaagagata atatggtaac tagaaaatct gaaaaagttc aaagagagtt
    25921 atattattct atagtggatg aagtagattc aattttgata gatgaagcaa gaactccgct
    25981 tataattgct ggtagtggaa aagaagacac taagttgtat gaacttgcaa atgcttttat
    26041 taaaacttta aaagataaag attatgagat agataagaaa gaaaacactg tatgtctcac
    26101 acacacaggt ctaagtaaag cagagtcttt ttttggtata gaaaatataa ctaatataga
    26161 taatatagag atttaccatc atacaaacca agcattaaga gcacataaac taatggagag
    26221 agatgttgag tatgttgtta aggatgaaga aatacaaata gtagatgaat ttactggtag
    26281 gatattagat ggaagaagat atacagatgg tcttcaccaa gctatagaag caaaagaagg
    26341 ggtaaagatt aaagatgagt ctaaaacatt agcaacagtt acttttcaaa atttctttag
    26401 attatatgaa aagctttcag gaatgacagg tactgcaaaa acagaagaaa gcgaatttga
    26461 atcaatttat aagttaaatg ttgttcagat accaacaaat aaacctgtta taagagctga
    26521 tttacaagat aaagtattta ggacagaaga tgcaaagtat aatgcagttg tagaagaagt
    26581 aataaagata aataaaacta agcaaccagt attagtgggt actgtttcaa tagaaaaatc
    26641 agaaatgctt tcaaaactat taaaaaagcg aggaataaag catgaagttc taaatgcaaa
    26701 acaacatgat aaagaagctg aaattatatc aagggctgga aaattaggtt ctgttactat
    26761 tgcaacgaat atggcaggta gaggaactga tatagcttta ggcgcaggag ataaaattga
    26821 agaaaaaaaa gtaagagata ttggtggatt atatgttata ggtactgaaa gacatgaatc
    26881 aagaagaata gataatcaac ttagaggtcg ttctggacgt caaggagacc cagggacatc
    26941 tagattcttt gttagtttag aagatgaaat gataaagtta tatggtggaa aaggaattga
    27001 aaagatttta aaaaggatga atgctaaaga tgaagatgca attgagagta aatcattatc
    27061 taaagccata gaaagagctc aaagaggaat agaaggtaag aattttgagc aaagaaaaaa
    27121 tgtacttaaa tatgatgata ctattaatga gcaaagaaaa gttatatatg aggaaagaaa
    27181 tagagtttta aatgatttaa atatatctga agatatacaa aaaatggtta gagaaataat
    27241 agaagatgca gctaataaat atttatctaa tcaaagagat tattatggtt actttaaatc
    27301 tctgtacaat gattttatgc ctccaaatac attgttgatt ccaggggtag ataaaaaaag
    27361 tgtagatgaa gttgtacaac acacatatga aatatccaaa agagtttatg atattaaaaa
    27421 aatggaagtt ggtatagaca aaatagaaga attagaaaag agagttttat tgcaagtagt
    27481 tgatacttat tggatggacc atatagatgc catggatcaa ctaagacaat atataggtct
    27541 taagtcatat gcacaaaaag atccatttaa ggagtatgct ttagaaggat atgatatgtt
    27601 tgaagaacta aatagaaaaa taagaacaac aacagttcaa tatttataca agtttgatta
    27661 attaagaaca caattcaaaa tagtttttag aattattttg aattgtatca agtaataata
    27721 taggatctaa ttatttaact tagaaaaaag aagctatatt taaaataata attattttaa
    27781 aatatagctt ctttttgtgt gagaaatacg gaaaaaagag atgattgaaa tttctttctt
    27841 ccttttaata ttatactata aagagtatta aaagtctatt agttataagt ggtaaaatta
    27901 aataaaatta caaaattgtt acttttatgt atacataaat atatgccaat attttacatg
    27961 gaaaaaaatg attaattttt gtataatgaa ataaagattc taataaagcc agagttatca
    28021 tatacattga aataatatta tatttgtatt ataatttata gataataagt aattttaaaa
    28081 atagaaatat acataattca tatatctaaa caaagtttat ctatgctatg caatatcaaa
    28141 tattttaatg ttgtgtatga tgattctaga gtcttgaaat ttttataatt acaatttata
    28201 ttgacactta agttgtttaa taagtaagag cattaaatga aatatattag aaattgaggg
    28261 gagtacaagt tatgaatata ccaaaaaaaa ttatatcgtt attgacaata acaataatgt
    28321 taagcggatt atctttaaat acagtaaatg cttcaacaaa agaatcttta gttggtagtg
    28381 gaagatggga gactgctata caaataagta aaaatggttg ggaaaattca aaagaagcag
    28441 tattagtcaa tgatagttca atagcagatg cattatcagc aacaccattc gcaaaagcta
    28501 aaaatgctcc aattttatta acacaaagta atagtctgga tgcaaggact aaagctgaat
    28561 taaaaagatt aaatgtatca aaggtctatt taataggagg aaatgttaca ctaagttcta
    28621 atttagagta tgagttgaaa agtataaata ttaactcaga aagaatatct ggagatagta
    28681 gatattctac ttcgttagaa ttagctaaaa agatagatag tataaacact atttctaaaa
    28741 ttgttgttgt aaatggtcaa aaaggtttag ctgatgctgt aagtgttgga gctgcagcag
    28801 ctcaaggaaa tatgcctata atactttcag atactactaa tggaactagc acttcaaaag
    28861 attttataag taaacataat ataacgacat cttatgtaat tggtggtact aattctgtat
    28921 ctgaggacat taaaaattcg cttcctaatg ctaaaagaat ttctggagaa aatagaaacc
    28981 aaactaatgc aaaagttgta gaagaatttt acaagggtac aaatttaaaa aatttatata
    29041 taactaaaga tggtatgaaa aaacagggtg acttaattga ttcgctagct gttggtgttt
    29101 tagctgctaa aaatagttca cctgttcttt tggcaggaaa taaattggac tcagttcaaa
    29161 aggatatact agataccaaa atttttgata agattacaca agttggtgga aatggaaatg
    29221 aagatgtttt taagtcaata caagatattc aagaagaaac taagtacaca gttgaaacaa
    29281 tttctgaact tgatacagct ataaaaaaat cagatgctaa tgatattata agatttaaaa
    29341 atgatgataa aataactggc tcaattgata taagtacaaa aaaagctata acaattgaat
    29401 tcaatggatt atatagtgga aaagtaaata taaatatacc aaatggagat gtatataatt
    29461 atggagatat gactggccag atggatatta aagatataaa aactgacact ttagtaaatg
    29521 aagggtcaat taatgagata actatatatg acccaaatgg ttgtaaaata gaaaatgaaa
    29581 aagatgctga tatttggaaa ataacattac tggatacatc taagaatacc tatataaata
    29641 atagtggaac tataactaaa atagttagtg atgcatcaaa tacaactata aaaaattcag
    29701 gaaagattga gttaataagt gggactaagt ctccagcagt aagtggtaat caagccactg
    29761 atgaaaatat aaaagataat gatgacaaag aagcaggagg aatttctgta tatgtagatc
    29821 catgttctcc tccaaaagaa ggaaaggtaa tgttaagaat accaaaagaa ccaagaagtt
    29881 ctagttataa catatactac agaattgtta aatctagacc atctgcaatg gatgtaggag
    29941 atactatatc aaaaaggggt tggactaaag tgactactag agattcattt atgattagtg
    30001 cgacagatgg atattatgta gaagctgtag aaatctatga ttctgataga aaggtaagta
    30061 agtggggaag atcaggaagt atagatgatg gatataacaa tacaacttca tcagcaacat
    30121 taagaagtta taaataaaat attgatatgc atataaatta agaaggattt tttgtggttg
    30181 ttagtttcta tgtatttatg atataaataa tttaaaaatg agttatataa acttgttaca
    30241 ttaattaatt ttaattattg tgaagttatt atatagctta tttttatgaa aaagtttaat
    30301 gtacaattta aggtgagtat actacttttg atataaaact tttaaaatta ttattcaata
    30361 aagttttaat aatttcaaaa gtagtgtata ctcattttat gaagaatttt aatgaaataa
    30421 gaataaataa ggaggaaaga gtaagttgaa tataactaat aaatgtgttt taggaatatc
    30481 tattttagct attttgagtt ttggattttc agcgtttcaa ggtaatgctc aaattactga
    30541 tagaaaaaaa gattatgata tatatctaaa agcgaactct gataaaagtg atgtaaaatc
    30601 gtctttaaat gatatttcta agttagagaa aaaatatgga gaaagtgatg cgttgagtat
    30661 atctaagatt aatttatatg aaaagatgaa tgattataaa aatttacaat ctgaaattga
    30721 gaaagtttac aatagatatc ctcaaactag aaatacagtt tcatttgtac ttttatattc
    30781 tgaagttgct ttaaaaaatg gaaacaaaga attgtcaaag aatatgatta atgaagcaat
    30841 taaattagga gcacaagatg cttataaaga aagagtggat aaggtattat cacaattaaa
    30901 ttaaaaggag aaatatatgt tagataaata ttacaataga ggaaaagttg aacaaataga
    30961 taaatttatt ttgtttatat tattatttat gatgtgtatt attccaataa taactcatga
    31021 atatacttct acaaactata gtcctatttt tacattaaca ttgtattctt caggagaaag
    31081 ggtagaaata tttaattttt ataaaacagc aatattgtat ttggggacaa tgata
//
LOCUS       DAGSWI010000039        30782 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.039, whole genome shotgun sequence.
ACCESSION   DAGSWI010000039 DAGSWI010000000
VERSION     DAGSWI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.039"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(220..924)
                     /locus_tag="MJF80_001597"
     CDS             complement(220..924)
                     /locus_tag="MJF80_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBZ0280266.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     VGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
     gene            complement(1278..2294)
                     /locus_tag="MJF80_001598"
     CDS             complement(1278..2294)
                     /locus_tag="MJF80_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBZ0280267.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            complement(2422..2928)
                     /locus_tag="MJF80_001599"
     CDS             complement(2422..2928)
                     /locus_tag="MJF80_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBZ0280268.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            complement(3087..3755)
                     /locus_tag="MJF80_001600"
     CDS             complement(3087..3755)
                     /locus_tag="MJF80_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280269.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            complement(4168..4674)
                     /locus_tag="MJF80_001601"
     CDS             complement(4168..4674)
                     /locus_tag="MJF80_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBZ0280270.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHDNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            complement(5045..6073)
                     /locus_tag="MJF80_001602"
     CDS             complement(5045..6073)
                     /locus_tag="MJF80_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBZ0280271.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            complement(6121..6981)
                     /locus_tag="MJF80_001603"
     CDS             complement(6121..6981)
                     /locus_tag="MJF80_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBZ0280272.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            7250..7459
                     /locus_tag="MJF80_001604"
     CDS             7250..7459
                     /locus_tag="MJF80_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBZ0280273.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            7459..7725
                     /locus_tag="MJF80_001605"
     CDS             7459..7725
                     /locus_tag="MJF80_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBZ0280274.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            7754..8326
                     /locus_tag="MJF80_001606"
     CDS             7754..8326
                     /locus_tag="MJF80_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBZ0280275.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(8561..9751)
                     /locus_tag="MJF80_001607"
     CDS             complement(8561..9751)
                     /locus_tag="MJF80_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBZ0280276.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(10011..10442)
                     /gene="dut"
                     /locus_tag="MJF80_001608"
     CDS             complement(10011..10442)
                     /gene="dut"
                     /locus_tag="MJF80_001608"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBZ0280277.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            10555..12783
                     /locus_tag="MJF80_001609"
     CDS             10555..12783
                     /locus_tag="MJF80_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBZ0280278.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKFVKGKESKFDKLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(12894..13379)
                     /locus_tag="MJF80_001610"
     CDS             complement(12894..13379)
                     /locus_tag="MJF80_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBZ0280279.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            13631..14854
                     /locus_tag="MJF80_001611"
     CDS             13631..14854
                     /locus_tag="MJF80_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBZ0280280.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            14858..17992
                     /locus_tag="MJF80_001612"
     CDS             14858..17992
                     /locus_tag="MJF80_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBZ0280281.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            18026..19324
                     /locus_tag="MJF80_001613"
     CDS             18026..19324
                     /locus_tag="MJF80_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBZ0280282.1"
                     /translation="MSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLIALT
                     DYKKQVVSNQDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(19613..19858)
                     /locus_tag="MJF80_001614"
     CDS             complement(19613..19858)
                     /locus_tag="MJF80_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBZ0280283.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(20072..22699)
                     /locus_tag="MJF80_001615"
     CDS             complement(20072..22699)
                     /locus_tag="MJF80_001615"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBZ0280284.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GNVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(22760..23602)
                     /locus_tag="MJF80_001616"
     CDS             complement(22760..23602)
                     /locus_tag="MJF80_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBZ0280285.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(23609..24247)
                     /locus_tag="MJF80_001617"
     CDS             complement(23609..24247)
                     /locus_tag="MJF80_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0280286.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(24683..25975)
                     /locus_tag="MJF80_001618"
     CDS             complement(24683..25975)
                     /locus_tag="MJF80_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBZ0280287.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(26012..26368)
                     /locus_tag="MJF80_001619"
     CDS             complement(26012..26368)
                     /locus_tag="MJF80_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBZ0280288.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(26403..26762)
                     /locus_tag="MJF80_001620"
     CDS             complement(26403..26762)
                     /locus_tag="MJF80_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280289.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(26831..28804)
                     /locus_tag="MJF80_001621"
     CDS             complement(26831..28804)
                     /locus_tag="MJF80_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBZ0280290.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(29026..30039)
                     /locus_tag="MJF80_001622"
     CDS             complement(29026..30039)
                     /locus_tag="MJF80_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBZ0280291.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(30052..30597)
                     /locus_tag="MJF80_001623"
     CDS             complement(30052..30597)
                     /locus_tag="MJF80_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBZ0280292.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
BASE COUNT        10455 a         5091 c         3699 g        11537 t
ORIGIN      
        1 aattaacaat atatttttat tgttaattat gcaaataaaa aagagtataa taaaagcctt
       61 taaaatacat ttttttctta ttttaaaggc tttttatatt ctacattttt attttctata
      121 ttatatcctt tgataatcgt tatatctaaa atagaccttc ttaccctaaa tataagtatc
      181 tttataacta gaatagtcca ttacaacagc acctacatat taatccactg atacctcttt
      241 attatcaata tctaaaattg aaattttaca attcccaccc aatttttctt gaactatttt
      301 agctaattca tttaaagcat tttcaaattc tttaaatcta ctttcatcta ctaattcaat
      361 gcataaaaag gcattgttag tatcttcagt aagcattgtt ctcactgctt cacgccaatt
      421 ccaacatcta caaatcgctc caacatcatc tttataaatt atttctcctt catatggaaa
      481 tgaattttca tctgtcccta gtgggataaa ctcctcatta ccaattgctc tagttagcct
      541 aatatctcct ataaatttat ctatatcctc ccctccacaa ggtaatgcat attttaatga
      601 gataaaattg tagatatcca ctaaagggtt aatagttcct atattatttc cattatgaac
      661 tctttttaat aatgcttcaa tagaagacct tgctcctttt ttcgttttaa attttttaaa
      721 tgcttctctc catactttaa tcacttggtt gttgctaaat tctgagtcct ccaagtattt
      781 taatgcttct tcttctccct tcaaaattat tttcttatag tcttcatctt ttatagagtt
      841 atctatatca ttacaaataa caactccaat ttttgcacta ggaaatagat tccaaaatgc
      901 atcctcaata acaaattttt tcattagaaa acctcctcaa atttaaaata tgtaaaaata
      961 aaaaagccta tagaaatatt attaaatatc tcccaggctt ttatccttcc gtgtacacaa
     1021 caattgtgcg ttttctcttg gacctgacca ttataaaatg cggaacccta gaaaactaaa
     1081 ttataagttt tttaatttac aaaatacaat taaattttag cataatgtag tatttaaaac
     1141 aatattatat tatctgattt gtacattaaa acaatttgct attaaattat aattatggtg
     1201 gcaaatgggt ggcaaatcac cttttttatt acattttcgt ttgtttaaac tattgaaaat
     1261 actatattct tacaatttta atcaatttca gatttaaatg gagtgtcatc agcttttgct
     1321 attctatact ccttatcagt tatataacca agctgatgca tcttatacag tacaatcact
     1381 tgtctcttct tagcctctcc atggtcaata taccttgcag gattatttgt cataccagct
     1441 aacatagcac actcagctaa atttaattca ctaacttttt taccgaagta gacttcagct
     1501 ccctttgcag ctccatatgc atttttacct agatacatat tatttaaata tgcttcaaga
     1561 atttcatcct tagtcataat cttattcatc tgcttagcat tatacatatc ccttattttc
     1621 cgcttcatag ttttatcatt actagtaagc aggttttttg aaacttgcat atctatagta
     1681 cttgcacctt gtgttgtatc tgtaaacaca ttgtttaata ctgacctaac tagagaaata
     1741 acatctactc ctttatgctt ataaaatctc tcatcttcta tggaaactat tgcatgtttt
     1801 aaatcatttg gcatttgttt tatacttaca acctcactac ttatatattt agacctaatt
     1861 aactttttag taacttctgg tgaccctttt atagctgaaa ctgtaaatat tgcacctgcc
     1921 actgaaaata taattagtgc aactaacata atagataata atgccctaac tattctaatc
     1981 cttttttgtt tttttctttt tttacttttt aattttttct ctctttgttt atactcgact
     2041 cttcttcttt ctctttgatt gtaatctgta tttaaaggtc ttacattatc tcttgtaaaa
     2101 ctatctctat ctctttttat aagattctgt gaatctactt tttcactcat tcttttactg
     2161 gaacttgtat taggactttt atttttttta tttgccgaag tataagaatg tttagatttt
     2221 ttattactat ctttgttgtt actaattttt ttacgtctta ttttattatt atcattatca
     2281 ttgtaataac ccattttagt ctcccttctt catataaggt acattataca ttgttctaca
     2341 aaataaatca aagaacaaaa ttgtaagctt aaggggcaat tttataaagg atattttcac
     2401 taattttctc tatatttata attaatattc atcttttatt ttcaaaaata gttttgcagt
     2461 tgctaatgta tcactgtcag ctctatgagc atttatattc tctatgttgt aataagcaca
     2521 agctgtttca agtttcttgt tttttccttt ataactttta cattttttta ataactctac
     2581 tgtacatata taattgttta ttggttctaa atctaacatt ctaagatagt aatttaaaaa
     2641 ttttaaatca aatttcgcat tgtgtgcaat tatagttcta tcacctatgt attttctaaa
     2701 tctaggtaaa actaattcta gtggttcttc attttctacc atttcattac ttatatgtgt
     2761 caattctgtt ataaatgaag aaattatccc cttaggtttt atcaaccttg aatagttttt
     2821 tacaatttgt tcatttttta tttctgttat tcctatttct attatttcac aacctttata
     2881 tggatctaat cctgtagttt ctaaatccac aactacatac tctttcatat ctcttctcct
     2941 tattattaaa tggatatatt tatattctat atttatgtaa tagactaatc aatacttaat
     3001 tttatatgat ataggaagat tttacaaatt acaacataga tatatcctat attccatcat
     3061 aaataaagat ttttttatta gaataattaa acttttaatt tattgttatt taatctattt
     3121 tgataaacat tatttatttt attattattt aaatctttaa aatctaaact tttacattgt
     3181 tcatcatctt cccatattcc tgcttgttta tttccatcat ttttatataa tacaccctta
     3241 ccatttttct tattatctaa aaaatatccc ttataaatgt cattattggg atatgttaat
     3301 tttcccaaac cattttttaa tccgtattta aattcacctg tatatattga accatctcta
     3361 taagtatatt ttcctattcc atgcatcttg tcatctttcc ataatccttc atacttttcc
     3421 ttacttgaaa aaatatatat tccaaatcca tttttctttc catcaacaaa ctcaccttta
     3481 tataaatcac cattatcgta tttaaatact ttttcttcct ttaatttctt attgttactc
     3541 ttatttttat tattattgtt atcctttata tgtatttctt ctcttctggc ttcaataaaa
     3601 tctctatcat acctaaatcc gtttcttgat ttaatttttt gattaatctt cttcttatta
     3661 ataaatactc tagctagttg agtaaatata aatacagatg ataagcatat aaatacacat
     3721 agtattgttg taaaataact agttatattt tccataaaaa atcacccttt aaaaaattta
     3781 tttaaaatac ctaatataat atatattttt attaaatgtt atttatactt ttatagtact
     3841 atattgaata aaattaactt tgaaattatt tacattaaaa taaatcaaat acaaatgcca
     3901 taaattaagt gtttctagtt agcataaaaa tacttataaa gtatactcaa atatttcaaa
     3961 taaatacttt tattcaaata aatctctact tatataagtg cttatataaa taaaaagatg
     4021 aactatagta aatatagttc atctttttta taatagtatt tatttaaaat taatatttat
     4081 ttagttacta gcatctactt agatatcaga taagaaagca cagtcattta cttataataa
     4141 gttcttttca tatatttttt attataatta ttcttttaca agaactttta taatttctga
     4201 aatgtacata gtatggtagt ctttctctgg ataccatttt ttatcatttt ctttagcatc
     4261 aaagttttct ggtggcatat tatcatgata tagcttttta catactataa tcatattagc
     4321 ttcttcaaaa gctaccgtat catccacaaa atatggtgta agtcctgctt tttctatttt
     4381 atttatatct ctaccagaaa tagtaccaca aatatttaaa gcctctctat acgtttcatc
     4441 aaagaaggca actgtaaatg tatcattact atctacaaat tcttttgtat atctctgtgg
     4501 tcttatgtaa gttgtaacaa catttttacc ccaatatact ccaacaccac cccagctagc
     4561 agtcattgtg ttaaactttt ctgaatcacc agctgttata agtaaccatt ctttgccaat
     4621 cttagtaaat ggattaaact gtaattcctc aatttttact tctctaaaac ccatgacttc
     4681 atcctaccct tcatttttta attatataaa ttaattgtta aaaatttgaa tatatgtact
     4741 cttataatac tgacttttga ttgtcctaca taattcatgt aaattataat acaacaaatc
     4801 aattactttc gcaataagta gttttcatat tttataagtg taaaatatag ataaaatata
     4861 ttgattataa agtatataat atatttttaa acctaaagca atatacaata aaaaaatgta
     4921 gaaagtaagt atctctctac attttttatt gtatattatt tttatattaa tttaatttta
     4981 tcttgattca gttttataaa aaattgttat aattgtcaag ttctactcta ttatttataa
     5041 tttcttatat cttattaaca acatttaagt tattacattt atttatatat attttttcat
     5101 tatctaagtc ttcatcaaac atttttatta tattttttat ctcatacttg tactcatctt
     5161 cattcattct ataaagcatt aaatcttcta gtacatcttt catatcgaag tacacatctt
     5221 ctaataaata aaaatagcct tcctctatat ctttcaattt atctatagta tccatttttt
     5281 tacttatatc tttcataatt tctaaatttt gtatatttac atctatgatt gttaataagt
     5341 acattctcac agtttttatt atttcaacag aaaaatctac aatattataa tttccttctt
     5401 ttttatataa tgactcctta aatttgttaa aacaattatc taaattagaa tttatatcgt
     5461 taaatggtgg tcttattatg tcacgtatta ttaaaagatt ttgtatatct acattatttg
     5521 tgattgcctc atccaaaata aataaattat atgcatcatt taatgggtca tgggcatctc
     5581 cactaaactc taagttaaca aattcaagta aatttttcaa tgatatatag ttcatacatc
     5641 tcataccaca atttatatat ttttctttta tatctacaaa taaatctcta atgttattta
     5701 caaaccttgg atggttattt tttttagcac ttctcatatc tgtgttattg aaacatgtca
     5761 aatccaaatt accaaaagta tatattcctt taatatcaga aaatatgcca agccattttt
     5821 taaactttct catgacctct tcataacttg gtgcactctt caattcttca gaagatatat
     5881 gtgttaattc ttgtatatga ggatacaact cctcattact tactggtctt attaaagatt
     5941 taaatttatc aatatttttt gttttttcat catacataac tgctccgatt gcaattatat
     6001 cagaattaaa tactgctctt tttgatttag aatttggcat attcatttca aaatctatat
     6061 atactctttt caatataaac accctttcca attgtttata gtgattcttt ttaactattt
     6121 ttattcaaaa ttttctaaga aagaatgttt ctccccatct ttttgccaac caagtactga
     6181 atcaagattt acattttctg ctgccttaaa tatagatttt tctacatttt taaaatttga
     6241 ttctaagctc tttattaagt cttttatctc atatctttta ttacctgttt ttttagcagc
     6301 aaccatacat gcacaattta atggccatat accactattt tgaataaatc ttataatatg
     6361 ttcttctctt atataataaa gtggtcttat aatttttata ccttcaaaat tagtagaatt
     6421 aagtttagga agcattgttt taaaatttcc agcacacaat aaattaagca ttgttgtctc
     6481 tattacatca tcataatgat gacctaatgc aagtttgtta caccctaatt cttctgcctt
     6541 tgaatataat gctcctctcc tcattcttgc acacatatag catggataat cttttgctat
     6601 ttcatctgct atctcaaata ttcttgaatc aaataaatgt attggtatat ttagatactc
     6661 acaattatct ataagtaatt gtcttatatt tgcatgatat ccagggtcca tagcaataaa
     6721 ttcaacatca aaattaactt gtccatgttt ttttaactct tgaaacattt tagccataag
     6781 aatactgtct tttccaccag aaatcgctac agctatctta tcaccttctt caaccaattt
     6841 ataatctctt attgccttca taaacttaga ccataaattt tttctatatt tttttattat
     6901 acttttttct atatctctta ctggaagtct ttcctcaaaa ggaactataa tttcgcagcc
     6961 ttttcctgat agttcactca tctaatcacc tcgtatattt atgttaataa tattttacaa
     7021 aaaagtctac cttcatccac aaaatactat aatttttact ttaattttac taaataattt
     7081 atagtttaca ataacacata aacttataat aacataaata acctctttct tttattatct
     7141 tttttatttt tctcatttaa tattttatta ttagtaacga tttttaaatg attgaatatt
     7201 atacactaga tagagttacc tatctattta caacaaggag gttttcatct tggaaaataa
     7261 gactagagat atcaaaaaaa attgtggtcc atctttggct agaccataca ttaataatca
     7321 aattttcaca gaaatgtact gcctttctga tgcattaaaa tttggaacta tatttccaga
     7381 actaaactta ctagagtctg aaatgtacaa caaagaatta tatgctaagc caaaaaaata
     7441 tagaggaggt agaagataat gaaagactca tctagagctg aacttatgag aaaagttcag
     7501 gaaacatctt ttgcatgtgt tgatatgaac ttatatttag acaatcaccc tgatgataaa
     7561 aatgctatca acacttataa ttcattatgt aatcaatttg cgcaagctag atatgcttac
     7621 gaaaataaat acggtccttt aactaacttt gggtatgctc caagtagatg cccttggcaa
     7681 tgggctgacc aaccttggcc ttgggacaga gaatttaatt attagacatc aagtattgga
     7741 ggattgaaaa ttcatgtgga tatatcagaa aactatacaa cacccagtta atataaaaac
     7801 ttgtgaccct agaatggcta aatttcttat aactcaattt ggtgggccaa atggggaact
     7861 tgctgcatct ttaagatatt taagccaaag atatacaatg cctactggta atatgcgtgc
     7921 acttttaaca gatattggta cagaagaact agctcacgtt gagcttatat gtactatggt
     7981 ttatcagtta acttctgatg caagcccaga agagttaaaa gctgcaggtc ttggttcaaa
     8041 ctatgctcaa aatggatatg gaatttatcc aacagattca aatggtgttc catttgatgt
     8101 aagacctata gcagttatgt caaatcccgt aaccgattta catgaggata tggcagctga
     8161 acaaaaagca cttgcaactt attatcaact tataaaccta acagatgacg ttgatgttat
     8221 agatgtatta aaattcttgg gtcaaagaga aataattcac tatcaaagat ttggtgaagc
     8281 tttaatggat gcttacgagt tagaagaatc tcaaaaaatg ttctaaaatt tttatttgta
     8341 taactattat tatttgataa tttataattt actgttgtgt taaaatttat aaaaaatata
     8401 aatataattt attttctata aacagagaaa ataaataaaa agcaggtatc aatcaaatag
     8461 taacatgtat tatactatac attactatca atattgtacc tgctttcata ttataagcaa
     8521 taaagaaaaa tctatttatt aggttttata ataaaaacaa ttataaaact cttcttgcat
     8581 ttacatatct acttgaatag tagctactat ttatattaga tattgttact ttttttgatg
     8641 aacctgaaga tgcatgtatc atatctccat ttcctatgta tattccaaca tgacttacag
     8701 aaccattatt tgaaccttgt gtatcaaaga atactaaatc tccacttcta aggtctcctc
     8761 tatttacata tgttccatac ttactttggt ctctagatac tcttggtata ctaactccta
     8821 cagatttttt cataacgtac tgtgtgaatc cagaacagtc aaaactattt ggaccttctg
     8881 ctccccatac atatggtttt ccaagtagtg tctttgcaaa acttattaca ctatcagctc
     8941 cttgacttgg agaattagtc gaataagatg gaattgaagt atcaacagaa cctttttcag
     9001 caaggtattg acttgacaca tatccagttt gattgccata acttactttt gtccatcctc
     9061 cactttcaga aatacttcca acctcagtac cataacttaa tgtagctatt ttagaatgac
     9121 ttgtacctgg tcctgttcta acgttcaatg actttgctgt tacaaccttt gtacccttta
     9181 caaggtcatt tgaactgttt tcagcatttt catttgtaga gtcatttaca ctagttccta
     9241 aatatttact tgaaacataa cctactctac cattagaact tatttttgac catccatttg
     9301 actcagatat gtatccgact tcagtcccat atcctagtgt tgatatttta gaactacttg
     9361 tacttggtcc tgttctaaaa tttaatcctt ttgctgttac aacttttgtc gacttaactg
     9421 aagtatcact actttcattg ctacttccaa ggctatttat tgctacataa tttccatgca
     9481 cataacctgt gtttccatta tatttaactt ttacccaaga accttctttt cctacgtact
     9541 caactttatc acctttgtaa agtttcccca ttgattcatg gtttgtacct ggtccttttc
     9601 taaaatttac cgcattccct gttattgttc tgctttctat actcacactc tctacttcag
     9661 actcattagc atatgctaaa ttccccatag taggcattat tatcgcagtt gccattatac
     9721 tggctgctat tctcttttga ttaaaattca taactttcca ctcatctcct cttttctact
     9781 atctttattt cacttcaaaa tataaaagaa ataattgaca ctatcaaaat atcaattatt
     9841 tcttttatat taatattatt acaaatttgt aactatttgt ctatagtaat atttgcttac
     9901 cataaattac catgtataaa ttttaatctt taatagcctg taatttagta atatagccat
     9961 cttttacctt ttttaaaaat ggctatattt tttaactttt ttgtaacttt ttaaaatcca
    10021 gttgagccaa acccaccttt tcctctttcg gagtcactta attcttttac ttcttctatg
    10081 ttaatatcat agataggttt tactatcatt tgagcaattt tcatatgctt ttcaacctta
    10141 aaatcatttt taccatgatt tattaatata attttaagct ctcctctgta tccttcgtca
    10201 atagttcctg gtgtatttaa tacagtaaca gaatgcttta aggcaagccc acttctaggt
    10261 cttacctgcg cttctgtcat agtaggaagc tctatacata tcccagtttt gataagttta
    10321 gtctctccag gaggtattac aacctcttct atagaataca aatccatacc agcatcacct
    10381 ttgtgagcaa aattaggtat tatagcatca tcatttaatt tttttacttt taaattgtac
    10441 atcttatatc tcctttcaac tttctataaa tattataact taaatttcga cctcttgaag
    10501 ttttttattt ttttgtggta atataattat tagtgtttta aaggaggatt ttttatgaaa
    10561 cttgtacttg ctgagaaacc atctgtagca aaaactatag catcattttt aggtgcaaag
    10621 accagacaag atggttactt tgaaggaaat gattatataa ttacttatgc agttggacac
    10681 ttagtatcat tatatgatat gaaggattat gataaagata aatactctgg ctcttggaaa
    10741 atgaacaatt tcccatttgt tcctgaggat aagtttaaat ttaagattga tagttctaaa
    10801 actaaacaat tcaacactgt aaaaaagctt ttgaatagaa aagatgtaga atatgtaata
    10861 aatgccactg acaatgaccg agaaggagaa cttatagcat ttttaatatt tttactggca
    10921 aaaaataaaa aaccagtaaa aagaatcttg gtaaatgaat ggacaccaga agatattaca
    10981 agaggtataa aaaacctaaa agatgaagat gaaatgagaa atcttcaagc tgctggatat
    11041 actaggctta ttactgactg gttgattgga ataaatttca cttctgttgc tacattgaaa
    11101 tatggaaatg ggaaactatt aaacataggt agggtaattc ttccaacagt taaattagtc
    11161 tatgatagag atatggaaat actaaacttt gttcctaaaa cttattatga aatagaaggg
    11221 cattttaaag ctgaagctgg tgaatataaa ggtaaatttg ttaagggtaa agaaagtaaa
    11281 tttgataaac ttgaagatgc aaataaaatt atagcttcaa ttacatctga aactggtaaa
    11341 atactagata aaaaagttac tatgtcaaag gaatatgcac caaaactttt tagtttgaca
    11401 tcacttcaag gatatataac ttctaagtat tccaatttta catctgataa ggttttaaat
    11461 gtctgtcaat ctctctatga gggtaatggt aagggtggat acataactta tcctagaact
    11521 gattccattt tcttagaaga aagtttggct tcaaaagctt ctcaaacttt agataaatta
    11581 aaggttggtc ttgaatatga aaataaaatt aaattttcta aaactaaaag agtttttgat
    11641 tcttctaaag tagactccca tagtgctata attccaactt atataattcc aaaaagttta
    11701 tctaaggatg aacaattagt ttatgatgcc ataaaagata gatttgtagc aaactttatg
    11761 cctcctgctg aatatgagaa tacagaaatt aagactgaag tagataattg tactttctta
    11821 acaaaaggta aggtacttaa atcaaaagga tatcttgaag tatataataa ggaagaaaaa
    11881 aacgatttac ttccacttgt aaataaagat gatgttgtag atgtgttaga aataaaacca
    11941 ttaactaagc aaactactcc acctaaacca tatactgagg acaccctttt aaaggctatg
    12001 aaaaattgtg gtaaaaatgt gcctgaagaa gatacaacag tgctatctgg atattcgatt
    12061 ggaacatcag ccacacgtgc tgatgtgtta aagaagataa gtcaagtagg ctatgtaaag
    12121 aaaaaaggga aatcttattc tataacagac cttgggaaaa atttagttga aatttttcct
    12181 gttaaagact tatttgatgt agattatact ggaaaacttg aaaaaagttt aagcgatatt
    12241 caaaaaggaa agtatacaag aaaagaatat cttactaata tcattagttt tatatatcaa
    12301 aatgtcaata ttataaaaca agatgcttca aaaaatataa acacagaagc atatactttc
    12361 gattcaaaaa ctaaaaaatt tactaaaaca aaagaaaaaa aacagaaaaa agatgtaact
    12421 aatactgaaa aagaagttgc tatatcaaag gattctaata acaaaaaaga agttaaaaat
    12481 ggtgatgaaa atcaatctct tggcaaatgc cccatttgtc aaggtgatgt actagaattt
    12541 gataaaggct ttgcttgtaa aaatcataaa gaatgtaaat ttgttatttg gaaaaatgat
    12601 aagtatttaa gtctttataa aaagaaagtt aacaaaacaa tggtcaaaaa tattttaaaa
    12661 aaaggtgaaa ctaaagtaaa atctttaact gctaaaaacg gtaataaatt tgatgctaca
    12721 ttaaaatatg ataaaaatcc agatactggc tatttcaatt ggaaaataga gtttgacaac
    12781 taaatattca taagaaaaga aatagcagta gtataataat ctatattata ttactgctat
    12841 ttcttttctt atgaagtttt aactttttta atagtgactt ttacaatcac actctaacct
    12901 aatttcttta ctatttctga taactttaat gtatgttcat ctaataaagt tagttcctct
    12961 tcagaaagat tagataattt ttttactaga gattctttga gaatagcttt tactgcatca
    13021 aataatttaa atgctttatc tgttaattca accctaagaa tccttctatc ttttgggtca
    13081 ggaaatttat tgactaaacc atgttccact aatctgtcaa taatcggagt catatttgat
    13141 tttaaaattc ctaaattaga agctatttga gaaattgatg atgaatttga tgtcataaga
    13201 taaagaatta ccttagcatg tgatggagcc attggacatt cttcaaaata agctttcatt
    13261 atatcagagt tattttgaaa cttttttaac aaatcgcttt ctttaaatat attattttca
    13321 agcacaaata aaaaatctaa aaaagagtcc gctattttaa gtatattgtt gttatccata
    13381 tttgtaacat cccttcaata gataatataa taaaacattg ttgtttaata tagtataaat
    13441 tataaactat taataaattt aaaacaagaa aaagttattg actcataata attataaatt
    13501 tataatcaaa ttagagaatt ataaattgcc aataaaggga agttttttaa ttagtctaaa
    13561 actaaactta aattataaga cactttaaat tctacaacaa gctagtgtgc aatatataat
    13621 aggaggagat atgaaaagat atttaatagt tttcttaatg tgtagttcac tgtttttagt
    13681 tggatgtgga aaaacagaag aatctgaacc tgagaaacca atagcagttt ctgttcaaaa
    13741 agcaataggt ggagaaattg aaaacacaaa ttctttctca ggaactacaa aggttaaaga
    13801 tgaaacagct gttactgcac agactgttgg cacagttcaa gaagtgtatg taaaacttgg
    13861 gcaaaatgta agaaaaggag atgaattatt gagtataagc agtccagagc tagaaaatag
    13921 tgtaaagcaa agtaaagcct cactagattt agcaaaagca agttattcaa gtgccactgg
    13981 tggttcatta gaggcacaag taaatcaggc aaaaacagct cttgacaatg caaagattca
    14041 atatgaggag tctcaaagaa attatgacaa taataagatt ctttatgaac aggaagtcat
    14101 tagtttagac caatttaaaa aaatagagtt ttctttagag caaacaaaac aacagttaga
    14161 ttctgctcaa agagcatacg atacagcaac ttcaaaaagt atacctcaag ctaaagcact
    14221 tgctaaaaaa cagcttgacc aagctcaagt atcatataat ttagcaatga gtaatttaga
    14281 caaattaaca ttaacatctc cagtagatgg tacaataaca gctaaaaact ttgattcaaa
    14341 ggaaatgata acacaatcac aaccagcttt tattatttct aatcctaata tactagaagt
    14401 tgatttaaat gtagcagaat cagatatagg taaatttaag aaagatggaa atgtagatgt
    14461 tataattgaa gaccaaagga ttttaggaaa aatagattat gtaccttcag tagttgaccc
    14521 tcagacatct ttatatcctg ttaaagtact tgtaaataat acaaacaaca aatttaaggc
    14581 aggtatgtct gctcaagtaa acttaagtgt tgaaaaagaa aatggtgctg ttacagtacc
    14641 taaaaaagca atatttgaag aaaatggtaa aaaatatgta tacatagcaa caaaagacaa
    14701 tatagcaaag aaacacttgg ttcaaactgg aattgtaaca gaggataaaa tagaaattaa
    14761 aagtggtgta agtgataaag atacagtgat tattggaggg ataagtctta tttctgacgg
    14821 aactaagata tttcctgtag aaaaggagaa gtagactatg aatttaacac agacttccgt
    14881 taaacgtcct ctcacaatca taatggtctt tttagtatta atagtttttg gtggtattgg
    14941 atataaaaaa atgtcaataa atctaatgcc tgatatagaa ataccagttg taatggtcat
    15001 gactacttgg acaggtgctg gtcctcaaga cgttgatgaa caagttagtc aaaaagttga
    15061 tgaaagttta tctgccgttt caaatgtaaa atctactata tcaagttcac aagaaagtgt
    15121 ttctatggta gttgctcagt ttgaatttgg aactaatttg gatgaaataa tgaatgatgt
    15181 taggtctaaa gttgatgcat tacagaccag cctcccagat gatgcagcta aaccaacagt
    15241 tttaaagctg gatatgaatg cacaggctat aggtcaactt gtcatatctg gaggaaatga
    15301 aaatagttca caagccttaa gaaaatacgc tgaagatgta attcagccta aaatagaatc
    15361 catagatggt gttacttcag cagaccttaa aggtggaaaa aaagcacagg ttaatgtaat
    15421 agctgaccca gctgttcttt caaattatgg agttagttta tccactataa aaggtgtctt
    15481 atcctctagt aataaaacat tcccttatgg ttcaattact caaggtgagg ataaaatagt
    15541 tttaagagca attgataaat tagaatccct agatgatata aaacagattc aaattcctgt
    15601 aaaaggagga aatacagtta gattagataa tctttgtagt gtagattatg gatatgcaga
    15661 caaagatagt atttatagat acaatggaaa agacagtttg gtaatagata tttctaaaca
    15721 acaagatgcc aacactgtaa aagttatgca gagtgcatac aaatacgtag atgaattgaa
    15781 taaggaaaat cctcaattta aaattaaaat tgctaatgat acaagttcat acataagtga
    15841 atcaataaac agtgtaatga gcaacttgct tataagtgct gttatcgcat ttattgtaat
    15901 atttgctttc ctaaaaagtg taagagcttc actagttgtt gcagttgcaa taccaatttc
    15961 tataataggt gctatagcaa ttctctactt tactggtgaa agtttaaatc tcattactgc
    16021 aagttcattg gtaatttcag ttggtatggt tgttgataat gctacagttg taattgaaaa
    16081 tatatttaaa tataggaaaa gtggcagatt gagtcttgat gattgtgcta ttgagggaac
    16141 aagaactgta acaaatgcaa tacttgcatc aacattgacc acgatagcaa tattcttacc
    16201 tatcatgttt acagaaggta tagctaaaat aacttttggg gcattaggta aaacactaat
    16261 agcttcacta gcattctcct ttataggtgc aattacactc gttcctagtg tatttgcaaa
    16321 attaagtaga ggaaaaaata gtcaaaagat gcaagaaaaa gatagtccta tatttgataa
    16381 ggtaagtgaa gcatataaaa aattattaag tgtatgtttg agacatcaac gcttagttgt
    16441 aatatcaagt ttacttattt ttgtagcttc cttatttgga atgggattta taggtatgga
    16501 ctttatgcct gcagcagaca agggtgaatt atctatttct atagaacttc caaaaggtct
    16561 tagcctagaa tccaatgatt actatgtatc tatgactgag aaaaaagtct cagatattcc
    16621 agaaattaaa actttaataa caactttgac atcagattca aataatagta aagcaagtat
    16681 agcaattgaa ttagaatctc aaaaaaatag aaaaagaact actgatgaaa tagaaaaaga
    16741 gataattgat agagtagcta ctgtaccaga ttgtaaaata aatgtaagtc aaaacagtag
    16801 tgttatgggt ggagctggag gagcagactt cacattagaa atgaaaggtc ctgatttaga
    16861 tgtattaaaa atactgggaa aagaagctga aaaagaattt agctctataa aaggatttag
    16921 aaatgttgaa acttctctta cagatacaac acaagaagct caatttacta tagataaaca
    16981 taaggctcaa gaatatggtg taaatactgc tgaaattgca ggcatgttac gtacagcaat
    17041 aaatggtgac tccgtaacca ctgctactat tgatgattac aaagtggatg taaacctaaa
    17101 atttaaagca gatactattg atagcttaga agatataaag caactaaaag taaactctgc
    17161 aactggaaaa gaagttcctc ttggtgcttt ttcagatata aaaatggcag atggtctaca
    17221 atctatatct aggactgatg gagatttttc tgtaaatata actgctacac ttgacaattt
    17281 agatgctggt actgctacaa aactagcact agaaaaaacg aataaaatgg taattcctaa
    17341 agattactct ataggtgttg gtggtgaaac agaaatgatg aatgaatcta tggcaagtct
    17401 tggattttca atggtcatag ctatagcatt agtttatatg gtaatggtag ctcagtttga
    17461 atcatttagt aaaccattta taatcatgtt ctgtgttcca tttgcattcg ttggagttgt
    17521 gctttcgctt ctagttagtg gtcaaagttt aagtactgtc ggtatgctag gtataatctt
    17581 acttatcggt attgttgtta ataatggtat agttcttatt gactacatag agcaacttag
    17641 aaaaacaaat cgtaacaatg atttaattgg tttggtttca attggttgtg cagcaagatt
    17701 aagaccagtc ttaatgacta cagcaacaac aattttaggt atgttgccaa cagcactatc
    17761 tcttggtgaa ggtggagaaa ctatgcaacc actagccata gttataatgg gtggtctaac
    17821 tgtatctaca cttataactt tggtgcttat acctactata tacttaatat ttgataaatg
    17881 ggaaaataag ttttcagaca aaatgcctaa aatatttggt aagataacag ataaaatagg
    17941 aagtataaaa cgtaaaataa taaagaaacc aaaattgaaa gctaaagttt aaatagaatg
    18001 gagggtgatt gtattgaata aaaaaatgag caaaatagtt gcaattggct gtggtgttgg
    18061 tataataact gccaatttaa tcccagttta cgcagataat aatacttctt ctataaacaa
    18121 agttgataat tctattttaa gtgactcaaa taaaagaaca tctgaaaagg aaattttgac
    18181 tgctagagaa gctgttagtg ctgcacttaa aaatagtgaa aaattaaaaa tgaaatcaga
    18241 agaaataaaa atgttaaaag aaaagttaga agtacaagat gagtttgata gttttactgg
    18301 aagtgacaac tcgtttccat acgaccaaat tggacttctt aaaaatcaaa gtgaacaggc
    18361 taaaggcttt atggaggacc aaatagcaaa tgatattact aataaattta acgatttagt
    18421 ttcaagagaa aatgagctag acaagataaa aaacaatctt gaaataaaaa ctaaagaaat
    18481 taaagatatg aaattaaaaa aagatttagg gttagtaaca tctttagaaa ctgaatcagc
    18541 tgaacttgaa cttcaaacac tacaaaatac tcaaaaagct aaacttcaag aactaaaaaa
    18601 caatcaagac tactttaagt tgttgactaa tatagattta aataattatc aattggataa
    18661 agaatataga tttgaatctt ttagagtagg tggctcagtt gattcatata tggaaggtaa
    18721 ggttaatgaa tatttaaaat acgaccaatt gattttagaa cgtacagaag aaagctttaa
    18781 tgataaagat gagaacaaag cagatttacc agatagacct aatcttacaa gaccagttgc
    18841 tcctacaaaa ccagagcaag gtaacctaac tgatgatgaa tataaggttc ttacggataa
    18901 gtatgaaaaa gactacaaga gatatgaaaa agaagttgaa gcatacaatc ttgaaagaca
    18961 gacttatgct gctggcctta ctacttatgc aaattatctt caacaaaaat ttaatactga
    19021 aaatggtcta gtaacattag aagacagtaa aaaagctctt aaaaaagggt taattgatag
    19081 ctatgctcaa ttacttgctt tagaagatac aattcagatt actaaaaaac aacttgattt
    19141 atcagaaaaa caattaaaaa atactaaatt aagatatgat ttaggattaa ttgctctaac
    19201 tgattataaa aaacaagtgg taagtaatca agatgctaaa aatagctatg acactcttat
    19261 tgtgaattat aactcactaa aaaatggtat agaaaaacct tggatactta atactggaaa
    19321 ataattctgg taaatagaaa gattacttta acatacgttt gattaaaaaa tagttatgca
    19381 cactcgtggc tttagtcatg gaagattcag aaggcaaata atagaaatag gttagtaaaa
    19441 atatatttac ttataaaact gtatactttt atagaggcta tctcaaaata gagataagcc
    19501 tcttttttat tgtttaaatt ataaaaatat atttgattcc atacaaaaag agtgccttat
    19561 gaactaaaaa gttcattttg agacaccctc aaaagcttaa ctctaaattt aattattttt
    19621 tatcaaatgt ctcatctatt ttacatctca ccatgttaac aaattcagaa atattatatc
    19681 ctctaagttt gcatccacat ttaggacaat aatccagttt cctagatacc aaagccctac
    19741 aactaggttc tggacatctt atttttagac tctcatctgc tactttaaat ccacatcgtg
    19801 ggcatatact acctggacgt gatgatactt cctttccaca tttaggacac tttgtcaact
    19861 ttatcccctc cagtaaactc aatttaattg atagttgaaa tttctctcta caataatata
    19921 tgcaatataa ttaaatacgt ttcacttaca actatataag ttaaattttt ataataaaaa
    19981 tcccctatat tcaatttaca aatacttaat ttgatacatt gaacaatagg ggattttatt
    20041 tttatttagt tacatatttt gcagtctatt attatctagg attttttata gatgcttgag
    20101 ctgctgcaag tctagcaatt ggcactctaa atggtgaaca agatacatag ttaagtccta
    20161 cactataaca gaactctact gaagatggtt ctcctccatg ctctccacat attcctagtt
    20221 ttaattctgg tttaacttct ctacctaatt tagctcccat cttaactaac ttaccaacac
    20281 cattttggtc tagtacttgg aatgggtctt tttctaatat ttcttcatct acatattgac
    20341 ctaagaattt accagcatca tctcttgaat atccaaatgc catttgagtt aagtcattag
    20401 taccaaaact gaagaagtct gcttcttgtg ctatttcatc agcagttaaa caagctctag
    20461 gtatttctat catagtacct acagtataag gaacttctat accttcttct tttattatag
    20521 cgtcaacagt ttctactatc atttttctta ttatttttaa ctcatttact tctccaacta
    20581 atggaaccat tatctctgga gaaactttaa cacctgcttt gattgcttct atagcacctt
    20641 gtattatagc tttagattgc attacacata tttcaggata agttattgca agtctacaac
    20701 ctctgtgtcc aagcattggg ttaaactcat ctaaatcaac tattctcttc ttaatatcac
    20761 ttacttttat tcccatagat tttgaaagtt cttctatagt ctcatcatca tgaggcaaaa
    20821 attcatgtag tggtggatct agtaatctta tattaactgg ttttccatcc ataactttaa
    20881 ataactctac aaaatcttct ctttgcattg gtaatatttt ttcaagagct ttttctcttt
    20941 gttctactgt attagataat atcatttctc taactgctgg caatctagct tcatcaaaga
    21001 acatatgctc agttctacat agacctatac cttctgcacc aaactctata gctgcacgag
    21061 catctctagg attatctgca ttagttctaa ccatcataca tttgtgtttg tctacccagt
    21121 tcattaattt ttcaaaattt ccagttaaag atacttctgt tttcttaaca tttcctaggt
    21181 atacatttcc tgtagaacca tctatagaaa tgtattctcc ttcttttata actacatcat
    21241 caagtgctct tatttcttta ttaaactcat caacttttat ttctccacat ccagcaacac
    21301 aacatttacc catacctcta gcaacaactg ctgcatgtga tgtcattcca cctctagctg
    21361 taaggattcc ttctgcttta accattcctt ctatatcttc tggagatgtc tctagtctaa
    21421 ctaaaactac tttttctcct ttttcatttg cttttactac atcatcagca ttgaaataaa
    21481 ctttaccact tgctgcacct ggtgatgctg gtaaaccttt tgctataacc ttggcttctt
    21541 ttaatgcctt atcttcaaat tttggatgta ataattggtc taattgatta ggctcaattc
    21601 tcatgatagc ctctttttca tcaattattc cagcttcaac taaatcaacc gctacattta
    21661 ttgctgctgc tgctgttctc ttaccatttc tagtttgtag tatatataat ctttcatttt
    21721 ctatagtaaa ctctatatct tgcatatctt tataatgacc ttcaagtata tgagtaattt
    21781 ttacaaattc atcgaataca gctggcatta catctgcaag agttgatata ttttgaggag
    21841 ttcttatacc tgcaacaacg tcttcacctt gtgcattcat taaaaattca ccaaataatt
    21901 tattttctcc tgtagctgga tttctagtaa atgcaacacc tgtaccacta gtttctccca
    21961 tattaccaaa taccattgat tgaatattta ctgctgttcc taagttatga gctatatcat
    22021 tcaatttacg gtatacgatt gcacgaggat tattccatga tctaaataca gcctctattg
    22081 caagcattaa ttgctctttt gggtcttgtg ggaaatcatt ttttatttct tttttataaa
    22141 ttgctttaaa ttcttttact atttctttta aatcatcagt tgtaagttct gtatcaactg
    22201 tgtatccctt tgcttctttt actctgtcta atacattttc aaatttatat tttggaactt
    22261 ccatagcaac atcagaaaac atttgtatga atcttctgta actatcatat gcaaatcttt
    22321 cattttgtgt agcctctgct aagccaacaa cactattgtc attaagacct aggttaagaa
    22381 ttgtatccat cattcctggc atagagaata ctgcaccaga acgaactgat actaataatg
    22441 gattcttatt gcatcctaat gttttattta agtctttttc taaagtagct aaatgctctt
    22501 ctatttcttt tattatctct gctcttatac tttcattgtt aacataataa tcattacatg
    22561 cttctgttgt tatagtgaat ccaggaggga caggtaagcc tatcttagtc atttctgcta
    22621 aattggcacc tttacctcct agtaaagatt tcatgtcttt gcttccttca ccaaaactat
    22681 aaacatactt agtttccatt gttaaatccc ccattaatct ttttatttta atttatatca
    22741 tttttattaa tagacattat taaatttcta tttctttgta tatctttaga ccattttctt
    22801 tcattatgtc taaaattata cctgctgttt cttctattgc cttatttgaa acgtttatta
    22861 ctgggcatcc aattcttttc attatctctt ctgaataatc taactcctga agtattcttt
    22921 ctaaattagc gtagtttgct ttactggaca atcctaaagc ttttaatctt tgggttctta
    22981 tctcattcag cttttcaggt gaattagtaa gacctattat ttttttagta tctatctcaa
    23041 acacttcttt aggaattggt atttctggaa ctaatggaac attagctact tttatatttt
    23101 tattagctaa atacatactc aaaggagttt tagatgttct tgatattccc actaaaatta
    23161 tgtctgcttg aagaacacct ctagggtctt ttccatcatc atatttcact gcaaattcta
    23221 tagcttcaac tctcttaaag taagattcat ctaattttct aattatacca gcttctcttt
    23281 taggttttct tcctgttctc ttgcttattt ctcttagtat atctgtcatt aaatctatac
    23341 aactaagacc ttctctttcg caatatcttt caacaatact gcataattct tcatcaacta
    23401 atgtatatac aattaaagcg ttttcagctt ttgcattctc caaagtttca agtagtactc
    23461 ctttattaat catgtatgga aatcttttta tttcataagt ttcattcaca taaaactgag
    23521 acatacatgc ctttgcaact tgttgtgcag tctcccctac tgaatctgat actgcatata
    23581 taattaggtt tttcaccagc atacctcctt aattgtcaac tatttctaag aatagcttag
    23641 ttatgttggt ttttgatatt ctcccaacaa ctctcatatg atttttgtct tctcccttat
    23701 attcaacaat cggaatcgaa tctacttcat gttctattat ttttcttgaa gctaataaaa
    23761 cacttgctcc tttagttaaa gtgactacat ttggagttct tgtcattatc atacctattg
    23821 gcattttatt tatatcagaa cctcctatag ttgcttttag caagtctttt ctggaaacaa
    23881 ctccacataa ctcttcattc tcatctataa taactatact ccctacatca gataaaaaca
    23941 tagtgactat tacatcatat atactttcat ccttcttagc tagtacaggc atgctcatta
    24001 tatcctctac tgttttttct tttacacttt taccaattaa atttatttca ctaactcctg
    24061 aataaaaata tccaactttt ggtcttgcgt ctaatattcc tgtcattgtt aatatagcta
    24121 aatcagatct aagtgtagca cgagtcacat tcaagctgga ggcaatactt tcactcgtta
    24181 ttggttcatt ttccttaact atatctataa tttttagttg tctttcatta agctgaataa
    24241 taatcacctt ctttcattgt gctatacttt ttatcttttc gcatcttata tagtatatta
    24301 tataactata tataccattc tgtaaacaga ttttttattg aatacgatat attttttact
    24361 aagttctctc gcttaaagat atttattata tattaatggg ggatatttct ttaattaata
    24421 gaactatatt agtattacag ttaatattat aatacataat tggtcatttt aattcatttt
    24481 atattaaaat ttttaataat attatttcaa tgtgaatata aaattgcaga actaattcaa
    24541 aataattttt acgactacaa ataacctata atatactttt aaagaaagta tcctaacaag
    24601 aatatataaa aatttaaaag ggttgattta aaaataacca tcaacccttt ttactaatat
    24661 ctatttatat taccatcaaa tcttacccaa atattttttc ttgcaattgt cttaaatgat
    24721 ataaagtagt atctttttct atctctacca tatcgtaagt tataacttca ccttttttaa
    24781 tatcttttac aactttagtc tttttactta tcaaaccaat aggtacagca ttcatagcct
    24841 ttgctacatc gtatttttct atagtaccat agcatgtata tgctccatag ccatccataa
    24901 actcaccttt tttcaaatct ttcttagcta tagtcataac ttctgctact agtccagctt
    24961 taggtactat tgtagcctta tggtctaaga ctgccatagc tgctgataaa ggagtctcta
    25021 aactacataa atgatatggt ctatataata tgtaattagg tccatcaccc atacttaaat
    25081 attttagttc atcattaact gcttttaatt tatgggctac tactacgaaa actccaggtg
    25141 ctataccatt tacaaaatca actacaccat aattatctaa tactccacct tcgcttttta
    25201 atgcatattt cttaggtact tcattaactg tagcttcaat accatgacca cctcttacat
    25261 caggcacaaa tccagttgca ttacacatta aagccatttc taccattgtt ttagttcctt
    25321 ctttaaaaga agctatcata tgaggtgaag ctccttttct ttctgcttct tctctaactg
    25381 tatcaggatt acaatctaaa tctaatttat tatttttacc tttacctatc actctaactt
    25441 caaaccccat tgcatctgca aaatcaaata attccattac tgcacctggc tcatctcctg
    25501 cagaacctgt aaatattact ccagcatcat ctgctaattt tttaagtatt ggtccaacaa
    25561 cacaatcagt ttcagcattt aacataacta tatgtttttt atttaaaata gagtttaatg
    25621 ctatctctgc accaattgca atacctccag ttgcatctat aactacatct attgacttag
    25681 ttttacaagc cacaagaaaa tcattacatg tcaatacttt tccttcttgt aattttattt
    25741 ctgcatcctt ttcatttgtt acattatcta taatttcttc atctattcct gcatcagtaa
    25801 aagctttatg agccaactcc aaatttatat ctacaacaat agaagcttca atacctttca
    25861 tatttgccat ttggcttacc attccatttc ccatatgccc agctcctata agagctactt
    25921 ttattttttt accttcctct tccaattttg caagttttct gtttaaatct aacataccaa
    25981 ttcctcctgt tataataatt ttattttcaa attaagaaat agttattaaa tctccaatag
    26041 ttatatctgg atttcctatt tccacatgta tttctcctgg taaatttact tcacattttc
    26101 catcaaattt gattgtacaa tgcccaattt ctctaagagt cttctgagca atatcaccaa
    26161 ctgaaactat acaataatct ctattcccta tagtaagtgt atctcctatt tcaacatcac
    26221 tttttaacgt tgctattgaa tgaattacag atatatcctt taagtcgtca tcattaatgg
    26281 tttcatcata tataataaga caattgcttt catccattaa ctctttaact aaactgccta
    26341 tcccacttat cttaacttca tatttcatga cattcctcct gataatttat atttatattg
    26401 agttaattaa aagaaacatt tattttttct tttataaatt ttatagcttg tacaatactc
    26461 tcttcatctt taaatttttg ctctgcacta tatatatcta atccttctat ttcttcaatc
    26521 tctttaaatt tagccattac tgtacgtcct tttaaaattt ctccaaactt tataaaccca
    26581 tctgaatctg aagatataat ggctatactt cctttagaaa aatagctttt ctttttgcct
    26641 gtagacacta tatcaccctt ttttttagct ctatttaaag ttttaaaata caatttattc
    26701 tgcttcatta taaatatagt atttaaaata taagcaacaa ataaaatcac taataatttc
    26761 atatttatac accttccaaa aatcacatta tttttaaatc tgtataacaa ctataaatgc
    26821 tgtacaatag ttatataagg tttttaagta acaccttttt aaattctaaa aaagttctac
    26881 atttacttat cttattcgtt aattttacat cttggcctat ctttaaaact tcttcataaa
    26941 tttttactaa taagtctgag tctatcttat tctcacatgg aaacattgtc ataagaatta
    27001 tttttatact tctatttgca tattctattg gttcttctaa aattccaact gccattaaaa
    27061 acttatctga tttttcattt acagaatgag gaaataataa acctttatca aatactgtgg
    27121 gagatttttc ttctctattg actatatttt ttctaaactt atcatcaatg catcccaaat
    27181 ccataagatg gtccatcatt ttttctaatg aagcaagtat agtcttttca tttaatatca
    27241 taaatttatc ttcatctaac aaattattta ttagtaaatt tgtagaatta gtagtgtcaa
    27301 tattcccttc tcttaggcac aatactgttt ttaattgttc tcttagtttg ttttcatcaa
    27361 atattgtatt tatttttaat actattgtat ttatatttga tgtgtctaaa tcaatagttg
    27421 taaaaactat atcgtattca tctaagttaa tatttctcaa attaaaactt gaaagagtat
    27481 caattttagg attatttcct atcagttttt ctactcttat tttcaatagt tttgctgttc
    27541 ctagacctgt actacatata attgctattt tttttattga atagaatcta ctgctatttc
    27601 tttctaaata tccactgaaa tggattgcta tatatccaat ctcatcttct gaaacttcta
    27661 agttatactt tttctctatt acttttcctg ctatttgtgc taatttaaat ggaagtatat
    27721 aattttgctt aatctcttct aacaaaacat tttttaactt tatattaaat aatagcctgt
    27781 ttaaagtaaa actaagatga tatcctagac ctactcttaa ttcatcctca tttataaaaa
    27841 tacccatatc gacatatatt tgattaaata tattttccat caactcatca atgtttttac
    27901 taattctact actatttaaa gcacattctg atgctggtgc atttccacta actaacggta
    27961 tagttaaata aataatttct tcatcattta aagtacattc aaacattcct gatattatat
    28021 gttttatttc cctagcaata aagtattcat catatgattt aagtaaatca aactttacat
    28081 cataatcttt tactttcttt tcttctttta ctctatagac taaaactact atgtagttaa
    28141 taacttctct taacatttca tctgtaacat aaaaattatt ttttttaaat aaagtcaata
    28201 gtttttcttc taaatttatg atttcttctt ttttgattcc tttaaaatac tttgaattac
    28261 tgaaatcttt ccttgagttt ttatacaact gatttaatat aaacagtcta atatcaagtt
    28321 catttccatt taatttcata cctgtattct gttttttaat caagtttaaa ttatatgaag
    28381 ccaaaacttt ttctacatac tggaaatcat ttactaaagt agttcttcct acacacatct
    28441 catcagctaa actatcaagt gtaataaatc catctagttc tatcaagtaa tttagtataa
    28501 actctactct atcttctttt gaatttaatg aaaatacatt ttttctattc atatcaataa
    28561 tcttattgag tatctgctca tcttttattt ctagtttata gccttgaccc tttatatatt
    28621 tgattactgc tatatcatta cctaaatctt tattcaattg ttttatatac gtccttacac
    28681 ttctagttgt gacattcaat tcatttgcta ttgagttaat actcacaaac tcttttttac
    28741 tacacataat caatatctta aatatcttgc ggtcaataga aggaattttc tcagttacac
    28801 tcattttgta tctcctcctt gatttcattc tagtacaaat ttgaaaccaa attaatacat
    28861 aaaatttcac tttaataaag gaaaatttag tattaatcta actcgataat tacagctaat
    28921 taatagtata aaagctagtc tttcattaac aaaaaatctt gactagctta aatttatatt
    28981 tacaatttta ttaattaatt ccaagcctta tattgacatt aaatattatc tcaatgcaaa
    29041 tactgcaact ccatatgcaa taataacagc tattggacca gttattaaac gagagtaaaa
    29101 taaagctgga acaccatact ctacagtttc tggttctgct tctcccaaac ttaatcctac
    29161 tggtacaaaa tcacatccag cttgcccatt tattgcaaat aaagctggaa gtgctaaata
    29221 tgctggtatt gcaccaagac caatttgtgt acctacaaga gtccctacta cttgagctat
    29281 aactgcacca ggtcctagta ttggtgataa gaatggaagt gtgcaaatta ctgatataat
    29341 caataatcca ccaatattac ctgcaagtgg agatataact cttgcaatcc agtctcccag
    29401 tccagatgct aaaataattc ccataagcat acttacaaat gccataaatg gtattacgtt
    29461 attcattacc atttggatag tatctcgacc tgcatcataa aattttgata ctacttgccc
    29521 tgccccttga ccaatagaaa gtattatgtt ctttttagca tattcacctt gtgcttttga
    29581 cttaacctca tcataattat caggctttct aatagaactc ttattttcgc ttttattttc
    29641 ttgagatttt tgtggcatat cttccccatc tacaacactt atacagttag gattaacatc
    29701 tgatacataa tattcttctg ttataaattt tgctaaagga cctgtttttc caactggatt
    29761 tacatttatt gtaggtattt ttttctttgg ataaactcca catctagcag ttccaccaca
    29821 atcaataata actactgcta cttcttcatc agaaactcca gttttaaatc catctacaat
    29881 ttcacaaccc agtagttctt ttatttttaa tgttacttca tggataccac cagcagtcat
    29941 tgatagtatc ttatttctat tcccttcttt ttttatatac aaaggacctc cccagcctga
    30001 agttcccttt tctattttta atattttatt ctcctccatt ttatctacct cctaagcctt
    30061 atttgttgct ctattttttc ttgttagaaa agcatacatt ttttctgtta ctatacctct
    30121 tataaaaatt acaattagac caactataaa ataccacaat gctagtgtag tagtttcttt
    30181 acctaaagta gtcaaacctg cagatattcc caaccataca aaaagttctc ctgcatttgc
    30241 atgtggaaaa agacctgtaa ttggatgtac aaatgaaact gctgcatcat aaaatcctgc
    30301 tttgtgctct tcttttaaaa atctaccaaa tgtatagcac ataggatttc ccaagaaaaa
    30361 tactgatact aatggaagta gaatatatct tgtaaaagag ttctttccta gcttagaagc
    30421 ccaattatta actctttcct caccaattat cttaatcaaa gagttaatag ctaccattaa
    30481 tacaattagc gttggaagag tacctgaaac aagacctata aattgttctc cccctttctg
    30541 aaacaaaccg ataaaccact ctgcaccttt acttagacct gtaataacgt tttccataaa
    30601 attataacct ccctataata tctttaaatt tttaatttta ctgcttacca taaattttac
    30661 aaattacatc ttttttctgt actatatgct aatattgtaa atgttttttt attaatatca
    30721 ttttaatata atatttttga taaataaata catctttttt caattttata aaggaaaaaa
    30781 tt
//
LOCUS       DAGSWI010000040        30513 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.040, whole genome shotgun sequence.
ACCESSION   DAGSWI010000040 DAGSWI010000000
VERSION     DAGSWI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.040"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(73..255)
                     /locus_tag="MJF80_001624"
     CDS             complement(73..255)
                     /locus_tag="MJF80_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280293.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            568..987
                     /locus_tag="MJF80_001625"
     CDS             568..987
                     /locus_tag="MJF80_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBZ0280294.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            1118..2959
                     /locus_tag="MJF80_001626"
     CDS             1118..2959
                     /locus_tag="MJF80_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBZ0280295.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            2986..4871
                     /locus_tag="MJF80_001627"
                     /pseudo
     CDS             2986..4871
                     /locus_tag="MJF80_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            4879..6879
                     /locus_tag="MJF80_001628"
     CDS             4879..6879
                     /locus_tag="MJF80_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0280296.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            7141..8400
                     /locus_tag="MJF80_001629"
     CDS             7141..8400
                     /locus_tag="MJF80_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBZ0280297.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLILVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            8461..10248
                     /gene="iorA"
                     /locus_tag="MJF80_001630"
     CDS             8461..10248
                     /gene="iorA"
                     /locus_tag="MJF80_001630"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBZ0280298.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVEHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            10250..10825
                     /locus_tag="MJF80_001631"
     CDS             10250..10825
                     /locus_tag="MJF80_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBZ0280299.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            10878..11948
                     /gene="buk"
                     /locus_tag="MJF80_001632"
     CDS             10878..11948
                     /gene="buk"
                     /locus_tag="MJF80_001632"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBZ0280300.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQCSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            12166..12867
                     /locus_tag="MJF80_001633"
     CDS             12166..12867
                     /locus_tag="MJF80_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280301.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            12927..13433
                     /locus_tag="MJF80_001634"
     CDS             12927..13433
                     /locus_tag="MJF80_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBZ0280302.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            13608..14630
                     /locus_tag="MJF80_001635"
     CDS             13608..14630
                     /locus_tag="MJF80_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBZ0280303.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(14777..15085)
                     /locus_tag="MJF80_001636"
     CDS             complement(14777..15085)
                     /locus_tag="MJF80_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBZ0280304.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(15145..15573)
                     /locus_tag="MJF80_001637"
     CDS             complement(15145..15573)
                     /locus_tag="MJF80_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280305.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(15796..17253)
                     /locus_tag="MJF80_001638"
     CDS             complement(15796..17253)
                     /locus_tag="MJF80_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBZ0280306.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            17670..18584
                     /gene="nadA"
                     /locus_tag="MJF80_001639"
     CDS             17670..18584
                     /gene="nadA"
                     /locus_tag="MJF80_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBZ0280307.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            18597..19898
                     /locus_tag="MJF80_001640"
     CDS             18597..19898
                     /locus_tag="MJF80_001640"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBZ0280308.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            19879..20715
                     /gene="nadC"
                     /locus_tag="MJF80_001641"
     CDS             19879..20715
                     /gene="nadC"
                     /locus_tag="MJF80_001641"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBZ0280309.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            20971..21303
                     /locus_tag="MJF80_001642"
     CDS             20971..21303
                     /locus_tag="MJF80_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBZ0280310.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            21465..21650
                     /locus_tag="MJF80_001643"
     CDS             21465..21650
                     /locus_tag="MJF80_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280311.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            21677..22762
                     /locus_tag="MJF80_001644"
     CDS             21677..22762
                     /locus_tag="MJF80_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBZ0280312.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQRYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            22755..23213
                     /locus_tag="MJF80_001645"
     CDS             22755..23213
                     /locus_tag="MJF80_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280313.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            23248..24339
                     /locus_tag="MJF80_001646"
     CDS             23248..24339
                     /locus_tag="MJF80_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBZ0280314.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            24344..24568
                     /locus_tag="MJF80_001647"
     CDS             24344..24568
                     /locus_tag="MJF80_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280315.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            24578..25141
                     /locus_tag="MJF80_001648"
     CDS             24578..25141
                     /locus_tag="MJF80_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280316.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYNKSVDALKNKATFKKVLK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            25102..25731
                     /locus_tag="MJF80_001649"
     CDS             25102..25731
                     /locus_tag="MJF80_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280317.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            25771..25908
                     /locus_tag="MJF80_001650"
     CDS             25771..25908
                     /locus_tag="MJF80_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBZ0280318.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            25908..26660
                     /locus_tag="MJF80_001651"
     CDS             25908..26660
                     /locus_tag="MJF80_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280319.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            26673..27158
                     /locus_tag="MJF80_001652"
     CDS             26673..27158
                     /locus_tag="MJF80_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280320.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            27165..27314
                     /locus_tag="MJF80_001653"
     CDS             27165..27314
                     /locus_tag="MJF80_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280321.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(27450..28271)
                     /gene="yidA"
                     /locus_tag="MJF80_001654"
     CDS             complement(27450..28271)
                     /gene="yidA"
                     /locus_tag="MJF80_001654"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBZ0280322.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYMQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            28764..30458
                     /locus_tag="MJF80_001655"
     CDS             28764..30458
                     /locus_tag="MJF80_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBZ0280323.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSHFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
BASE COUNT        12025 a         3143 c         5349 g         9996 t
ORIGIN      
        1 agtttatgtc aatctttttt gttaatgaat aaataatata gaatacaata tacaaaaaag
       61 tttcttgaaa tgctaagata atccccattt acaaagcagc atcattatac tgtgtaaatt
      121 tgaatcaaca atgttttcac catatttcat cataaaatcc tctaattgtg tgtcaataaa
      181 tgaataatat ccacttatac tatcgatgta tgcagtcatt aagtgtatac attcatcact
      241 tttatcttta tacatatata tctcccccta tatacaatat ataaatttag ataccaaatc
      301 gtatcatatt aaatagttaa gtgtgtaaca tctatgcagt tatatattta tatcattata
      361 ttaataacta aagtgagttt tgacaataga ttttgggaaa ctggatgttc aaagtgcaaa
      421 cttggatttt aaaatcatta attaagatag cgcataggaa tatatgtata cataacttaa
      481 ttataaagat tataaacaat tacttttatt aactttattt aaatggtaga ataacttgga
      541 gattaataat tataaggagg atataatatg agtttatata agtttatata tgatgataag
      601 gaatatttat taaaagagga gaactgtagt gctttaataa atgatgaaga aaatccagtt
      661 caaggagtaa gtatttcaaa aataattgat atacttaatg aagcagaaga agttgatttt
      721 gatgtagaat attatcaaga agcatgtcca ttatgtttag aaggagtaaa agaaaagaaa
      781 aaattctttc cttttttaga atatcacttt tatattttca gtaaagatgg agaatatgta
      841 ataagcaata tatctgttga ttataaagga ctatctttta ataaactatc aagggcaaat
      901 aaagttgata atagttatat tgtaagtgtg ataatttgcg aaaattgtca ggattatata
      961 gttcagatag aaaattgtat agtttagtgt aaagtatgtc ttaatttaag agaaatttct
     1021 aaattgagac atcaactttt ttcaaaaata gtatggaaaa ttactaaatt tatgagataa
     1081 tgtaagtcgg aggtgagtta caatatatag taaatatttg gaagagatag atgatacaat
     1141 tttagattca atgtttaaac taaccttgaa tgaaagatta ttgactaata acgattttat
     1201 tgaaaataaa attgatgtag taaagaattt aattgattgc tataaaactg aacaaaatag
     1261 tttaatgtta ttaaaatcaa attttttgct agctttatta tatggtattc aagggtttag
     1321 tgaaaaaatg aaagagtata ttttgatttc ctgtaaatat attgataaat gcttacccaa
     1381 agattataaa tttttagcac gattttattc acaaatagct attttaagtt taaaaaatga
     1441 agatgtaaat aaggcaaatt tatatatgga taaatttaat cttatttgtg atgaaaataa
     1501 ttttctaatt gaagaaatta tttttaaatc acaacttatt tacattaagg caagtaagtg
     1561 tatagaatct tctgtaataa ttaaagaaat agaaacgttg tatataaaga taaaagaaat
     1621 taacgatttt aattgtaaaa aaatatattt ttttattatt ggtaaaatat attttttgtt
     1681 tttggatgat gctctaattg ctaaggtaaa ttttttaaaa gcaagaaaat atgcagattt
     1741 actaaaggat atgcaagtat cttcactatg taatataaag ataggtgagt gtgaatgttc
     1801 gtttgaaaat tatgaatgtt ccatggaata tttcaatgaa gtaataagca ataaaaagta
     1861 tagaaatgta aatataattc aaaaatatag agcatcaaac aacataacta aaatattaat
     1921 aaagactaga aattatcctg tcgcaataaa taatttagca aaatctgaag tatatctaga
     1981 aaaaattaaa aatcatagct taaaagaagt agagaagttt gatttattta taagtttagc
     2041 catgtattat gcaaaaagta atgaaaaatc ttttgaacaa tcaatatgct atttagatag
     2101 agctaaaaac atattaaata tgataaaaaa tatagaagaa tatataatat atgatttaga
     2161 aataatttat caccaggcaa agatatatta tatctttgga tattatgaca atgccttaaa
     2221 cacaaataaa aaactccttg aaaaatctaa aaatgctaaa aattataatt atgtgaagtt
     2281 agcatacaag agtatatatt tgtgttttga aaagattcaa aattatgaaa tgagtatgaa
     2341 atactttaaa atgtattatg agttaaaaat gatgtatata aaagctcgaa atagaaatta
     2401 tatagattcc ttaaattata gacatgagta tatagaaaaa gaaataaata acatgagaaa
     2461 agtaaaactt gatttagata aaaaaaagta tgttgaccat ttgacaagtg catataatag
     2521 aacttttttg aacgattttt tagaaaaaca agaagttagt gaatattgca cagcatttat
     2581 gatagatgta gattacttta aagaatacaa tgatacttat ggacactata atggagatat
     2641 tgctcttaaa aatataacta taataataaa aaaatatcta aaaaaagaca tgcttatgat
     2701 aaggtatggt ggagaagaat ttctgatatt atctgtatgt aaagattata aaaaaagtaa
     2761 aatatttgga agaaaattat gtaggattgt aaagaaattt ttaaacaata atttgacagt
     2821 tagtattggc attgatacat gtaaaaatag tagtatagat ataaaagaaa taattgaaaa
     2881 tgctgataaa gctttatata aagcaaagga aagtggtaga aatagatgtt tgcattatca
     2941 tgattttaag aatttttaac tgttgaaata ggattggtga tttacttgaa aaggagttta
     3001 atagaagatt taaagagaaa tataaacaag atagaatacg atatgttgac taaattctat
     3061 ctaaatttga cagatttaaa ctacatcttg gattatcaca aagacctttt aaatacaaaa
     3121 ttagaagata atagtagagt ctatacttta tatttaatta gtcttataaa ctatataaaa
     3181 aaagatagag aagcatcata caactatttg atagagtcat taaagtatat taaaaaattg
     3241 tcattaaaag atgattcaga tataatatct aaaatttata tttatcttac tttatcagct
     3301 atttctataa gaaaatatga taaggtaaat aatttttatt tctcagcaaa aaaattataa
     3361 gacaaaatca tttaaatgag ttgttggtat tgttaaatat gagtttatgt gtagaaataa
     3421 gtgctttata tcagaccaaa acggatgtaa tagctttagt agaagaagtg tcttttttta
     3481 aaactttaaa aaataaagct cttgtaagta gggcaaatta tatatttggg agagtttatc
     3541 tatatttatt taataacttt ataaaaagta tggaatactt aattgaatgt ctaaaattgg
     3601 ctcaagaaaa taatttatac tctatagagg ctttttgtaa atgtataata agtttgtgtt
     3661 atttagagag tggaaataat gtagaagcta taaaatacat aaataatgta ttagatgttg
     3721 cacacagtaa taactcaata tcagtaactg aaagagtgtc tataaagata gaccttatat
     3781 gggcatattt aaaagaagat atgaataaag aagcagaagt tattttatta aagactataa
     3841 aattaatgaa agttgttgaa ggtgtatata aggattattt atattcattt atatttttat
     3901 attcagctga aattgagtta aagaaagata attgtagttt tgaaaaggta aacacctatt
     3961 taattttatc aaaaaatata tataaaagtt ttggagatgt ttttatatat aattctaaac
     4021 tagattacat atataagttg tatggagatt tatatataaa atttggtaat atagatgaag
     4081 gtataagttg ttatataaaa ggtttaaatt tagttaaaaa gagttcattg aatcttagaa
     4141 aaatatctat attttatggg ctaatagcaa atggatatga gctaaaaaaa gattttaaac
     4201 tggcaataga atattataaa agtatggatt ggtattctga taggtgggaa aaaaataatt
     4261 cctataaaac atctcaagca attcacaaac aatatgaact cagacaaaaa caagagagtt
     4321 taagattact agtggatgat aataagaaat tagaaaatga tattttaaaa gatggactta
     4381 ctaaactata taatagaaga tatttggaac agcagttgga tttatttaat ggaaccagag
     4441 acaaaaatat ggtcgcaata ctggtcgata tagattgttt taaaagttat aatgataatt
     4501 atggtcatct agcaggagat aaagttatag ttaaggtaac tgatattata agaagtgtat
     4561 ttatagatat taccgaacac ataatcagat atgggggaga agaaatttta atattagtag
     4621 attctataga atcaatcggt gttgaagaga ttgataatat agaattatat attaaaagag
     4681 ttttcaaact tcttgaattt gagggtattg aacatatata ttctaaaata aaaaactata
     4741 taactataag tgctggagta agtataaaaa ggtgtcgtag tagaaaagat gttgaaatat
     4801 taattgaaga tgcagataag aacctatata aatcaaaaaa agcaggtaga aatcaataca
     4861 ttataattta aaattgaggt gattttgttg cgaaaaaaaa taggaattat agcctctgat
     4921 attgaattaa aagaaagaat agaagaatta tatagagaag atgtagaaaa tggaactata
     4981 ataatcgaca tacttaacct agacttaatg gaaaatcaag gtcgtatact tgttgaaaaa
     5041 ggtgcccaag ctataattgg tagaggtgga ggttatagct tagtaataga tacagtaaat
     5101 gtacctgtta taccaatgaa tatgaagtca actgaccttt taagagcaat agaaatagca
     5161 aagaaatatt ctaaaaaagt agtattgatt cttggagaca atgaagtttc ttttgactat
     5221 gttggctgga gaaatgtcat aagtactgaa ataactgaag aatggtttga atctaagtat
     5281 gagataagaa gtaaagttgt aaaatatata gaccaaaaag atgaagttgt aatcgtagga
     5341 ggaggtttgg catgttcttt tgcaaggcaa tatggaattg atagtgtatt tgcaactgct
     5401 agtgatgaat ctataagaga agctgtagaa tattgtaaaa aattattaga tacattaggt
     5461 gaagaaaagt ttaataatga agtgttacga aatatacttg atggtataaa agatggagta
     5521 attgctatag atagtaatgg cagtataatt ttgtacaatg aaagtgcaaa aaacatgctt
     5581 aaagtagaga gaaaatgtgc acttaataaa tacatcttag atgtatttcc taaaatggag
     5641 tggatgttag attgtttaca tgaaaaagag gacgtagaag atagaaagat aagaaatata
     5701 aataatctaa ttgttaatac taggacaaca ttgataaaag ttgataatag tacttatgga
     5761 gttttaggaa taatacaaga tataaccaag ctacaaaact tagaaagaaa aataagattt
     5821 gacttgaatc aaaaaggtct ctatgctaga tatacttttg atgatttttt atttaaagat
     5881 aaattaacta aagaatttat tgaagaagct aaaaaaattg gaaaaagtga ttatacaact
     5941 ttactttatg gagaaagtgg gtctggtaaa gaaattatag ctcatagtat acacaacata
     6001 agtaaaagaa aagataggcc ttttgttgct ataaattgtg ctactatagc agaaaattta
     6061 cttgaaagtg aactttttgg ctatgaagaa ggtgcattta ctggtgctag aaagggtgga
     6121 aaaagagggc tatttgaact tgctcatgga gggactcttt ttttagatga aataaatagt
     6181 ctatccttta atattcagac aaaactatta agagtaatag aggaacgtca aataatgaga
     6241 attggttctg attatataat acctctggat ataaggatta tagcagcaac gaacgaatct
     6301 ttaaccgaaa aaatagttat gggtacattt agagcagact tattttatag attgagtagt
     6361 ttagagataa atatacctcc tttaagagac agaagagaag atataatacc tttgtttaac
     6421 aattttgtaa atgaagtatt aaaggatgat ggtctcaatg gaataaacag tatagatgaa
     6481 aattttgttt tgactaaaga tgaaatggat aagctatata attatagctg gcctggaaat
     6541 gtaagagagt taaagacaat agctcaaaaa tatgttgtga caggtaaaat taaattgaga
     6601 caagatagag attttaaaac taaaaaatta cttcctaact ctgaagtaga caaatttaat
     6661 tctgaaacga cagcaagtgt agaagtccaa gatgaatcta taaatataag taagataaat
     6721 gatggtaaga taagtataga tataaaagaa gtaaataagt atgttgaaga aaaaataata
     6781 agtatgcttt ttgctcaagg actttctaaa aatgaagttg cacaagttct tggaataagc
     6841 aggacatctt tatggaagaa atacaataaa aatatttaaa tataattaca tataaattat
     6901 tttgaagtaa tgttaatttt ttaaaacaag tgttaagaaa aattaacatt acttttttgc
     6961 aaatttttat ttttttagac aggcatatat atttataaga aaaatagtta aaacacttaa
     7021 tttaataatg aaaaaaataa taattctaaa caaaatcaat tttacataaa gtgggattta
     7081 ttggcatagt aattgcttaa tatataatgt aagagtaaat acaattttag gggtgatggt
     7141 atgagtcaaa gtatggttgg taagcatgca atgtggccaa aagaaaatga tgttatattt
     7201 agcatatctg gcagagctca agcagcagag aaggcttttg gtatggataa tgtaataaat
     7261 gcaacaatag gcgctctaat ggatgattca ggaaaattaa taacaatgaa aactgtatat
     7321 gaagaatata aagcattaga taactgtgag ataggtgcat atgcagctct tgaaggtcaa
     7381 ccagattact tagaagcagt taaaaaagta tttttcagag attacttacc agaaggacat
     7441 ataagagtat tagcttcacc aggaggaagt ggtgcaataa aattagctgt atggaactat
     7501 actaatgaag gagatgaggt attaacatct gattggttct ggagtccata tgtaagtata
     7561 gctgaagagg ctaatagaaa agtagttaat taccaacttt ttgatgaaaa tagaaggttt
     7621 aattttgagt cgtttaaaga aaaatttgta aatatagcag aaaaacaagg tagagtcttt
     7681 acaattataa acacaccagc gcataatcca actggatata gtgttgcaga tgatgagtgg
     7741 gataaaatat tagacttatc aaaagaagtc gcaaaagata aagacaaaaa gataatattt
     7801 ttcgtagatt cagcatatat tgactttgca ggtgatgatg atgtatgtag aaagtttttt
     7861 aagaaatttt ctaatttgcc tgaaaacgtt ctcatattgg ttggattcag tatgtcaaaa
     7921 ggatttacag cttatggtat gagaatgggg gctataattt gtatatcttc aagtgaagat
     7981 gtagctgaag agttccatta ctcatgtgtt cattcatgta gagcaaattg gtcaaattgt
     8041 aatagaagtg ccatggcagt attatctaac attgtaaatg accctaaaaa attcaaagaa
     8101 tatgaggatg aaaaagaaat atacaaaaac atgcttacta gaagagcaga tgtatttgta
     8161 aaagaagctg aaagagtagg attagaaatt cttccatata tagcaggttt ctttgtaagt
     8221 ataccatgtg ataatccaaa agaagtatgt gaagaattga ctaaacataa tttatttgca
     8281 gttccattaa aaatgggctt aagatttgca gtttgtgcag tttctgaaga taaatgtaag
     8341 aaagcaccaa gtattataaa agaagcactt gaaagtttag aagtaaaaat aaataattaa
     8401 tttcattgaa caaattgggt ggactatgaa tttaataatt gtagatggag ggaattttat
     8461 atgaaacaat tgatgacagg taatgaagca attgcacgtg gagcctatga ggctggtgtt
     8521 aaatatgcat ctgcatatcc agggactcca agtacagaaa tattagaaaa tatagcaaca
     8581 tataaagatg caatagttgc agagtgggct ccaaacgaaa aggtagcact tgaggctgct
     8641 atagggggtt caattgcagg agcaagaaca atggcttcta tgaaacatgt tggtttgaat
     8701 gttgcagctg atccaatatt tacttatgca tatacagggg taaatggtgg tatggtactt
     8761 attacagcag atgagccggg aatgcattca tctcaaaatg agcaagacaa taggatgtat
     8821 gcaaaatttg caaagatacc tttatttgaa ccatcaacta gtcaagaagc taaagatatg
     8881 ataaaagaag cttttgaagt tagtgaaaaa tacgatacac cagttttata tagagttaca
     8941 acaagacttt gtcattcaaa aggattagtg gagtgctatg atagagaaga agttgaaatt
     9001 aaagagtatg ttaaaaatgc taagaaaatg gtaactgttc cagcaaatgc tcaaataaga
     9061 aggggcgttg ttgaagaaag aatggaaatc cttaaaaaat tctcaaatga aactgattta
     9121 aactactatg aaataaatga tacaaaaata ggtgttatag cctctggtat gtgttgtaat
     9181 tttgccaaag aagtttttgg aaaaaatgca tcatatatga aactaggatt tacaaatcca
     9241 ctaccatatg aaaaaataaa agaatttgca gaaaaagttg ataaaatata tgttatagaa
     9301 gaaaatgacc catttataga agaacaaata aaagcatatg gaatagattg tattggaaaa
     9361 gatgtaatac caccatatgg agaaatgaca ccagatgttt taagaaaagc tatatttgga
     9421 aaaacaaatg atactataga atacaaatct gaattagtaa caccaagacc accaagtttc
     9481 tgtgcaggat gtccacatag aggattcttc tatgaacttg gaaagagaaa aaatttaata
     9541 gttggtggag atataggttg ttacactctt ggatttgctc ctccatataa tggaatagac
     9601 tatgttgttt gtatgggttc tgcttttgga actgcacatg gagctcaaaa agttcttaat
     9661 atgaaggatg ataatgaaaa aagattggta ggtgtacttg gagactcaac attcttccat
     9721 acaggaataa atggactttt agatgttgtt tacaatagag gtaattctat ttcggttata
     9781 ttagataata gaataactgg aatgacaggt caccaagaaa acccaggttc aggatatact
     9841 ttacaaggtg ctaaaactaa agaagttgat atagaaggat tagttaaagc ttgtggaata
     9901 gaacatgtaa gagttataaa tcctaacaat ttaaaagaag taaatgaagc actagattgg
     9961 gctttagcta tagaagatga accatcagta ataataacaa gatggccatg tgtacttaaa
    10021 aaattctcta aagaagatat agaagaattt aataatccat ttaagactaa gtgtaaagtt
    10081 gagcatgata aatgtatagg atgtaaatta tgtttgaaaa ctggatgtcc agctctatca
    10141 tttgataaag agaataagtt atcaaatata gatagaaatc agtgtgtagg atgtggagta
    10201 tgtgcacaag tttgtccaaa acaagcaata gttaaggagg aaaaataata tgactaagag
    10261 tatacttttg gtaggtgtag gaggtcaagg aactatactt gctagtaaat tattaactat
    10321 gggattgatg gaagctggat atgatgtaaa gatgagtgaa atacatggaa tgagtcaaag
    10381 aggaggttca gtttcctcac aagttagata tggagattgt gttcattctc cagttataga
    10441 aattggtgga gcagatatat tagtatcttt tgaaaaaatg gaagctttaa gatggttcaa
    10501 ttatttaaaa ccagaaggaa aagctttagt aaataatcat aggatagatt ctatgactgt
    10561 tttaatcgga ggagctgaat atcaagaaag tgatatagat gcagagttaa gtagattaaa
    10621 tgcaaaagtt ataaatgcag ctgataaagc tgaagaacta ggaaattcaa aaataatgaa
    10681 tgttatttta ttaggatgtc tagttaaatc tatgcaatta gagtctattg attgggaaaa
    10741 aattataagt gaaaatgtta aaccaaaatt tgttgaatta aacataaaag ctttccatga
    10801 aggtatggaa atggttagca aatagtttat aatagatata aatagactat agaaattaag
    10861 gtgaaaggaa gaaaattatg acttacagaa tattagccat aaatccaggt tctacttcta
    10921 caaaaatagc agtatatgat ggagaagaac aaattcttgt gaagacgata gaccatccag
    10981 ctgaagagat tgcaaaatat aatactatac aagaccagtt tgaaatgcgt aaggaagcag
    11041 ttttgaatat tcttaaagaa aatagtatag acttaaaatc tcttagtgca atagtaggaa
    11101 gaggtggagt tttaccacca gtaaaatcag gagcatattt agtaaatgaa gaaatgattg
    11161 atgtactaag acatagacca gtacttgaac acgcttccaa tttaggtgct gttgtggcac
    11221 atgcaatatc agaacctctt ggaatcaact catatattta tgattctgtt gcagtagatg
    11281 agcttataga tgtagcgaga atatctggac tttgtggaat ggatagatca agtgcagggc
    11341 atgcattaaa tactagagca atggctttaa aatatgctaa ggataaagga aaagattata
    11401 agagcttaaa cttaatagta gctcacattg gtggaggagt aagtatttat cttcatgaaa
    11461 aaggaagaat ggttgatatg ctatctgatg atgaaggacc attttctcca gaaaggtcag
    11521 gaagagtacc tgctacaaaa ttagtggctg cctgttattc aggtcaatgt tcagaaagag
    11581 aaatgactaa aaagataaga ggtaaaggtg gtatagtttc atacctaaat actgtagatg
    11641 ctagagaagt tgaaaaaatg atagcagaag gaaatgaaga agcaaaaatt atttatgaag
    11701 caatggctta tcagttagca aaaggtattg gagagttagc aactgtagta gatggaaagg
    11761 tagatgctat aattataaca ggtggaattg catattctga aatgtttact tcaatggtta
    11821 aaaagaaagt tgagtttata gcaccagtag aaattatggc aggagaaaat gagttggaat
    11881 cacttgcttt tggaacttta agagtactaa atggagaaga agaagctaga atttatagtg
    11941 aaaattaata agaaaaacta aaaatgttta tatactaaaa aagtggtata tccgtttttt
    12001 atacacgaaa gcttagagta taggtgaaaa tgtataaaat ttggattgcc actttttata
    12061 attaaaattt tatcttaacc taataaataa ttctgtaaaa tttcgtattt atatatatat
    12121 tttattggta tttattgtta ttattattat gaggagtgat ttttgatggg aaaaataagt
    12181 ttaaaaagta tgataagatt attttcaggt ttttttatat ttgcaatttc ttctgtgctt
    12241 atgattaatg cacatgtagg tcttatgcct tgggatgtac ttcaccaagg tttatctata
    12301 aagctaggca taactatagg tcaggcttct ataatggtag gagttgtaat tgttatattg
    12361 gatgcagttt ttggtgaaaa tataggatgg ggtactttac tcaacatgac atttatagga
    12421 atttttattg atttagtaat attttctgga gtgatacctc atgcaagcaa cacttatata
    12481 ggtgtgttta tggttgttat aggaattatt ttggcagcta tagcaagttt tctatactta
    12541 ggtgtttgtt taggaagtgg accgagagat gggcttatga ttgctcttca gaaaaaaact
    12601 aataaatctg tacgtcttgt tagaacaatt cttgaaatac tagcattagt tgttgggtgg
    12661 ttattaggag gttctgtagg tattggtacg cttgtaagtg ctttaggttt aggatatgta
    12721 ttacaaatag tgtttagaat atttaaattt gacacaaaat tgttgaaaca tagatttatt
    12781 atagatgata taagagaatg gaaggaaaag aaaagcaatg aacataaatg taaatcaagt
    12841 attgtaataa aaaatgagca gaattaatta tgctaaaata tatttgtagt ttatctaatt
    12901 tatagtaaag tcaaggagat tttgaaatgg aattatggga tttatataat gctgatggaa
    12961 ttaaaacagg aaatgttata aaaagaggta attctattga agaaggttat taccatttgg
    13021 cagttgaagt gtggatttta aatagtaact ctcaaatttt aattcagaaa aggtctaaaa
    13081 gtaaaaagac attgccaaat atgtggggaa tgacaactgg atgtattgtt tctggggaag
    13141 acagtttaga aggagcaatt cgcgaagcaa aggaagaaat agggatagat ataactaaag
    13201 atgagatgaa agtttttaga agtatgattc atgaagatac tctttgggat gtatacttag
    13261 ttaaaaaaga atatgatata tccaaagcta ttttacagga ggaagaagta agtgatataa
    13321 agtgggtaag cacagatgaa ataagacaat tactaaaaga gggtttgttt tttgaatatc
    13381 cagagatata cgaattgcta tatgagatag ataataataa attaaatata tgatgtagag
    13441 cagatttcag tataagtaag attatttaaa atgtatcttc tctaaatttt aatattgttt
    13501 tatgaaaatt aataagttat ttttagtatt tttctacttg ttttttgaga ttaaaataaa
    13561 aagggcagtc attataaagt cataaaaatt tggggaggat ttaaactatg gaagataaca
    13621 aaggattggc acgtgacagt tcaaaatata aaattgtttc tatatctgtc gtagtaatta
    13681 ttctaattac attttggtat atgttaaata tggtgctttt aacatttatt atgactttta
    13741 ttttctataa cttgcttgta gccacaagaa aacgaattaa gaagttttct tctttaaata
    13801 ttccagattc attgataatt atagtgttat atgcactatt tgctatattg ttagtattga
    13861 ttagctatgc agttgtacca ataattatag ttcaacttac agagttgagt agggttttta
    13921 gtgattttga tgtaaatcaa tttgctcaat ctttaggacc aaaattgtat cctattgtct
    13981 caaagctgga ttttaataaa tatatttcac aagcagggct acttatagca tcaacagcaa
    14041 caaaagttgg aagttttgga gtaaatatat tattggcatt tttactgagt ttactccttt
    14101 tacttgaaaa aaatgaaatt aaaaattttg gagataaact ttctgatagt aagatttctt
    14161 ttatttataa ttcgcttgta ttttttggaa agagctttgt taaaaatttt ggtgaagtaa
    14221 tgaaagtaca agttatgatt gcctttataa attctgttgt ttctatgatt tttttaggtt
    14281 tcatgggatt ccctcaaata tgggctttag gatttatgat ttttgtgttg ggtcttattc
    14341 cagtagcagg agttatagtg tctttgattc cattaactgt aatagcattt aatacaggag
    14401 gaataacaaa ggtttttgga gttttactta tgatttgtat agtgcatgca gtagaaacat
    14461 acatattaaa ccctaagttg atgtcgaata gaactaaact tccagtttgt tttgttttta
    14521 ttattttact tgtaggagaa cattatttag gtgtatgggg attactaatt ggagttccaa
    14581 tatttatgtt ccttatggat attctgggtg ttaaatttac ttctaggtaa tatattaaaa
    14641 taataattta tctttatata taaattataa attgttttaa aaaagaacct ataaatagac
    14701 atcattaata aagacatcta tttgataggt tcaattttgc tttgtataaa tactataaaa
    14761 ctaaataaaa agaaatttat ttcatttcaa aagtttcaca acttgcattt ctttcattta
    14821 tatgaacttt ttcagctttg caaagttcat ttttattgta tttacatttt acagcttcac
    14881 aatgaatgtt atctgtatca acttggttag tttcattaga acgactagtt ataccagatg
    14941 tatatttgtc ttcaaaagaa gcacaataag tattttctgt agtagttgct tgcattccat
    15001 ctacttttat actaccagca taacaatatc cactagtatt ataagcacaa ttagtagcag
    15061 cacaatttaa attattattt ttcataaata acctcctaag ttttatttaa acttattatg
    15121 tgaagttatc taaattttat acacttattt acaattacat ttatcacatt taacaactac
    15181 atctttggaa gtttcccttc ctaaattttt attaacatga tacacatcaa cattttcagt
    15241 tgatccacat atagaacatt taaaattgtt ttctagtagg gttctttctt taaataactt
    15301 ccaatacata gaattgatgt aatcattata atttaccttg aggcatgtct caagaaagtt
    15361 tatgtataga ttgtctaaat ctcctgtatt taattctgta tattctggtt caaaatattc
    15421 acaacatcct ctaaaatcgg gctttgtatc tgaaataaca catctaatat catatctata
    15481 gtctaggcta ggccccttaa atgaaagatg tgtgcaaaaa gaacaagatg tttctggtag
    15541 atttttttca agttcatgta atttattatt cataagtatt cctccaaaaa atttatttta
    15601 agtatataaa cgaatagatt attttaaaac ttttatttat ttatattaga gatttaggat
    15661 aaatattcca ataaggagta aatacttaag tatttattac tgtattaaat aaaaaagagg
    15721 caaatctaac ttgagattgc ctactttttt atcatttttt aataaaattt attctaagta
    15781 ttattttatt tagatttagt cagccaattt agtatctgct ttgcttttta actttctacc
    15841 agagcgtaca gttaaaatag catataatac agtaaatatg gcagctaaaa catatgatat
    15901 atttatgttt aagttaaatc caatttgagc attaaatata taactgaata taacaaagga
    15961 ataaaacata ccaggaatta agcttataat ataattcttt tcttttatta taaggtaaac
    16021 tgtaatcatt gcaaatgcaa atatggcaat agtttgatta ctccaagaga agtatctcca
    16081 caatatatta aatccagaag cactaagctt tgcaaatata aggatggcta tgacaggaat
    16141 aaatatacag attgacaata taacacgatg tttcttttcc ttttggtcat aatgaaagta
    16201 atctgcaagc attaatctta aagatcttaa agctgtatct ccagaagtta ttggtaaaac
    16261 aattacacct attatagcta tgataccacc aatagaacca agtaaatctc ttgcaaccaa
    16321 acctattaca tcaggagaac ctactaattc cttaggtatt cctttattgt aaatacccat
    16381 agcagctgca gcccaaatca ttgcaattaa accttctaaa atcatcatat tataaaaagt
    16441 agtttttcct tctttttcat ttgaaactga tcttgctata agagtagctt gagttgagtg
    16501 gaatccagaa acaataccac aagcaactgt aacaaagaat gtaggaataa gaggaatacc
    16561 atcaggatga atacctttcc aatttgcaag acttaaatta gctaaatcat aaccttgtgt
    16621 aaagattcca attccaacac cagcagcaga taaaagtaga atagctccaa atattggata
    16681 tacctttcct atgattttat caattggaaa tagtgttgca cacaagtaat atagaaagat
    16741 aagtccataa actatccaga caactggatt atttatagtc gatttcatat ttaaaatttg
    16801 agtaacaact aaatccccag gtgtataaat aaaaactgca ccaactaaaa gcattaaaag
    16861 acaaaggaat atattataga cttgaaatac tttattacct aaaaatctac tgataagaga
    16921 aggcatttgt gctccttttt ctcttatact aatcattcca ctcatatagt catgcaaagc
    16981 tcctccaaat acacagccaa taggtattag tatgaatgct attggaccaa acaatatacc
    17041 ttggataggt cctagaattg gtcctgtacc agctatatta agcaactcaa ttagactatt
    17101 cttccatttt ttcattggga catagtctac tccatcactt tgagcaagtg caggtgtttt
    17161 tctgtcatct gggccaaata ctttttcaca ataagcaccg tataaagcac ctcctattag
    17221 taatatagcc aagccgatta agaatgttgt cataatatca ccccatttat aaaatttttt
    17281 ggtctttgta tacatattat ctaaatgtgt agatttatat tgaatttaat tctggacaac
    17341 ctacaaattc tataaagtat tacaactaat ataatttcag aaataaatac atatacataa
    17401 ccatgtactt agtatacagg tatttggtaa tattttcaat gttatagcaa taaagaatat
    17461 aaacaaaatt ctgataatat tttacaaaaa aattaaaaaa tataaaaatt agtttaaata
    17521 gatggagaaa aaggaaaatc aattgagaat ataaatgtct atatcttaac aatattaaga
    17581 ggtttaaaat tcttgaagaa agaatagtca agtgatataa tgggtttaca ggtgtatata
    17641 cactagtaca tatagaaagc ggtgcaatta tggataaaga tttaacatat caaatcaaag
    17701 agctaaagaa agaaaaaaat gcaataatac ttgcccattt ttatcaacca ccagaaatac
    17761 aagaattagc agatgcagtt ggtgactcat attatttaag tgagattgct agagattgta
    17821 aagaagaagt ggttgtattt tgtggagtaa ggtttatggg agaaagtgct aagatattat
    17881 cacctgaaaa aactgtattg atgccagtaa gtaacgctgg gtgtgctatg gcagatatgg
    17941 tagatgaaga aggcgtgata aaattaaaac aacagtatcc aaatgctttg gttgtatgtt
    18001 atataaactc tacagctaaa gttaaggcac attgtgatgt ttctgttaca tcttctagtg
    18061 caattaaaat actagaaaac atagataata aagaaattat attccttcca gataaaaatt
    18121 taggaggata tattgcagaa caatttccag ataaaaactt tatattttgg gacggatatt
    18181 gtaaatacca taacaatata agagctgaag aaataattga gttaaaagat aagtataaaa
    18241 atgcagaagt attagtacat ccagaatgta aaaaagaaat aagagattta ggagattatg
    18301 taggtagtac atcaggaata ataaaatatg ctactaatag taaaaacaaa gactttataa
    18361 tagcaactga agaaggtata ttacatgaat taaagaaaaa taatcctaat aaaaactttt
    18421 atattcctgg tggaaaaata ctatgtacag atatgaaaaa gacaacatta gaaaatttat
    18481 atagtacact taaaaatatg gaaaatgaag ttattgtaga agatgaaatc atggaaaagg
    18541 ctttaaattc actgcttaac atgcacaaat tagcagaagg atagagggat agacttatga
    18601 atttagaaca agatgtttta atagttggaa gtggagtttc aggattatac tgtgcattaa
    18661 atttagataa aagtttaaat gtgctagtag tatcgaaatc aacaattgaa aacaacaata
    18721 catatcttgc acaaggtgga atttcaactg ctagaaatat tgatgatata gaatcctttg
    18781 tagaagatac aatgaaggct gggcagtata aaaatagagt tgaagcagtt caagtacttg
    18841 cagatgaatc tatagaaaat gtaggtcaga tagtagaata tggtatgcct ttagacaaag
    18901 aaaatggaga gatagattac acaagagaag gagcacatag tgtaaatcga atagttcata
    18961 gtaaggataa tactggagaa gttgttttta aaactttatt aaaagaagct aaaactagag
    19021 aaaatataac tcttatagaa gatgcatatc ttttagatat attaaaagat ggcaataaat
    19081 gtataggagc tagaattttt aaatctaaaa aagagattca tgttttttca aaaatagtag
    19141 ttttagctac aggtggaatt ggtgggcttt ttaaaaattc tactaatcaa agacatctta
    19201 caggtgatgg aattgcaata gctttaagaa ataatataaa aatagaaaat ttagaatata
    19261 ttcaaataca tcctacagct ttttatgaag aaaataatga aggtaggaga atgcttatat
    19321 ctgaatcatt aagaggagag ggagctaaac ttttaaataa aaataaagaa agatttgtgg
    19381 atgaacttct tcctagagat gtagtttcaa aagctatttt tgagcaaatg gaaaaagata
    19441 aattacctta tgtgtattta gatgcaacac atttagatag tgaatattta attaatagat
    19501 tttcttttat ttataatgaa tgtttagcta gaggaacaga cataacgaaa gaatgtataa
    19561 aggtatctcc tgcacaacat tattttatgg gcgggataca tgtagatttg gattcaaaaa
    19621 catctatgga aaatttatat gcagttggtg aaattagttg tacaggtgtt catggagcca
    19681 atagattggc aagtaattca ttacttgaag ggcttgtatt ttcaaaaaga gcagctaaaa
    19741 atataaactc agttatagat aatgtaaaag ttaagtttat agatgtacca gatatggata
    19801 tagatataga acaggtaaaa aaagaaaata aaatattagt cataaaagaa attgaaagaa
    19861 caagtgagga ttttggcgat gaattatttg attattgata gaatgataca ggatgctttg
    19921 atagaagatg ttccaagtga agatataaca actaattcta ttgtggatga aaattctaaa
    19981 tctactgtag atttaatttg caaacaagat ggtataatag ctggcttagg tgtatttaag
    20041 agagtttttg agatacttgg tgatgtagat gtaaaattat ataaaaatga tggtgataga
    20101 gtaaagaatc gtgaaaagat agctgtatta acaggtagta ctagaaactt acttgtagga
    20161 gagcgtgtag cattgaatta tttacagaga atgagtggta tagctactat tacaagtagg
    20221 tatgttgaaa agcttgaagg tacaaataca aaattattag atagtagaaa aacgatacct
    20281 aatcttagaa tattagataa gtattctgtt aaagtaggtg gaggatgcaa tcatagattt
    20341 aatttatctg atggaatatt gcttaaagac aaccatattg gggctgctgg tagtgttaag
    20401 aaggcagttg aattagctag aaaaaatact tcttttgtta gaaagataga agtggaagtg
    20461 gaaactttag acatggtaaa agaagcaata gaagctaatg cagatataat aatgttggat
    20521 aatatgagtt tggaaatggc aaaacaagca gtagatatta taaatggaag agccattgta
    20581 gaattttccg gaaatgtaaa tttagatact atagaagata taggaaaaat tggtgtggat
    20641 gttgtttctg taggagcgct tacacattct gttaaagctc tcgatataag tatgaagaat
    20701 ttgagaaata tataaatttt atattgaaat aaacttaatt aaaatagact ccttaaattt
    20761 gttttataaa tttaaggagt ctatttttta ttcattaaaa ttaataaaaa tagaaaaaaa
    20821 tcaatcaata atttaataaa aatagataaa tactatatac acttttaata atatagttaa
    20881 tatctatttt acagataaat aagaataaac ttataatata attgtatctg taaaattttg
    20941 taaaatataa aaagtatagg gggaataaaa atgaaagtag cttatatttt tagtagtact
    21001 aatacacata agattctaga caaaatgata ataccacaac ttgaacaagg aactcatgga
    21061 gtagacgttc tagggatgtt tttctttatg gataatacct tatttttatc taaaggtaat
    21121 cctgtaggcg agagactaag taaattacat gaaaaaactg atatgataat aatggcttgt
    21181 gaccaatgtg ctatagaaag aggtatagag aataatttgg ttgatggagc tacaattgga
    21241 tgtttcccaa acctatatgc ttgtttaggc agtgcaggtg tggaccaagt tataacgtta
    21301 taaattagta taggatacaa tccatagaaa aatttatttt ctgtggatta ttttaagtaa
    21361 aagataaatt gtttaaagta ggggattaac aaatgtctaa aattttagtt gaatttataa
    21421 acacttgacc atcttgtgat gaacatggag tgtttataaa aaaaatgtca gaaaagtatg
    21481 ctgataaatt ggaaataaaa ttatatcaag cagggaaaga tttttcgtat ataaaaaaat
    21541 atgggattat aacaaaagga actttaataa taaatcagaa aaagaaatat gatagattaa
    21601 ataaagatac aattgaaagg gcaatagtag aagctataaa caataattaa aagtttaaaa
    21661 attaggagta tcaaaaatga atatattaga aattgtttta aatggagtta aagatactat
    21721 agtagcttct atagttacac tgaatggagc atcaggatgg cttatgataa gttttatatt
    21781 ggcaggtgta cttcacaatg ttttaactcc aagaaggttt caaaaacaac ttggaaataa
    21841 gaaattttct tctttaatta aaagtactat ctcaggaatg tttttaccaa tatgtagttg
    21901 tggagtaata ccacttggaa taagtatgta ttattcagga gcatatttag gaccagtact
    21961 tgcttttatg acatcaactc caataataaa tccaatagct gtagttcttt gtctaggtct
    22021 tttaggtcct aaagttacaa tcatttatct aatagctggt tttttagtac catttttagt
    22081 aggattttta ggaaattgtt ttggaaaaga agaattatca cttcataatg attcagagga
    22141 agaagtaata gaattagaag aagaaaaaag aagttttatg ggaaatatga ttgatggtct
    22201 taaatggtct tttggtgaat tagcccttac tattagtaaa tatgtagtaa tgggtatggt
    22261 ttttgcagga tttataacaa ctatttttcc aaatagtata attcaaagat atttaggaaa
    22321 tccaggtttg ctatctcttg gaagtatagc aatacttgca tgtgttatgt atgtatgtgc
    22381 tgtaggacat ataccgttta tagcagccct tgttgcaagt ggagcatctc ctggtatagc
    22441 gataacattt ctaatagctg gagcagctac aaatctacca gaacttataa gtatgtttaa
    22501 acttattgga aaaagaactg ttattatata ttctgtaact ttgacactgt cttcaataat
    22561 aattggatat ataacaaatt tacttttagt gaataataac tcagtaaatt tagatgcaac
    22621 aaattcttca atacagttgg caaataaact aatgttaaac atacctgata gttttgagta
    22681 tgtatgtact attttaatta ttttatttgc atttaaatca ataataccaa agatgaaaga
    22741 ggtcatagga aatcatgctt aaaaagatag ttataatatc ctgtatagta gttgtgttaa
    22801 tcattttatc aaaaatagtt gatgataaca tcaaagaaga tgcttctatt ccaaatgtga
    22861 ataaagaaac attagaatat tttagaaaaa attataaaga agacataata acttgtgctg
    22921 aagaagactt aaataatgat gggaaaaaag atttagtagt tatatataaa aaaagcaata
    22981 attcaaatga aatggtagtc gtagtttctg ataaaaatag ccactatata acaaagccaa
    23041 taccagcacc tatagagaat caaactataa cctttaaaaa tattgatgat aaagcaccaa
    23101 tagaagtaat tgtatcaggg tctaaaaatg gtaatgtagg atatgctatt tacagagtag
    23161 agggtaaaaa atttgttgat ttatttggcg aagatatgga caaatgttgc taaaaataaa
    23221 aataatacat aaaggtggga aatttatatg aaattatcta agaagattca agcattaatc
    23281 ttatttggtg taatgggtac atctatatta acaggatgta gtagtaaacc aaaagaaaaa
    23341 gaagaagcaa aaactggaaa agatgattat acaataacag taggttatta taattgtgac
    23401 catatgactg cgggaccagt agcagaatcg gcaggaatat ataaagattt aggtcttaat
    23461 gtaaaaactg ttggaaatgg taaagttcct gaagctatgg cagcaggaaa aatggatgca
    23521 ggttacatag gaactaaggg gttagttgga gcaataccta agggttcgcc aataacaata
    23581 gcagctaaca accatactgg tggttctgaa tatttaatag tttcaaaaga tataaaagaa
    23641 cctaaagatt taataggtaa aaaaattgca acagatatga gtgatttctt atggacaagt
    23701 gattatggtc cagaaacagg tctaccaact gacccatcaa aatatgaagt tgtaaatatg
    23761 gattcagaca aagataaata tttagctctt aaaactggga aaattcaagc ttttacatct
    23821 tgtgaccctt ggggttctgt agctgaaaat gacggtgcag gaaaaataat agcatctact
    23881 caatataaag aaaaggctaa tggcaaagaa tataactgtt gttcattctc attaaacaaa
    23941 aactttataa aagaacatcc agatttagca aaaaaattgg ttctagcaca tacaaaagca
    24001 atagaatata tatatacaaa tccagctgaa gcagctaaaa tatttgcaaa atattataat
    24061 gtagaagaag aagttgcact tagaactatc tataagaaga ctgttggaga aggtagaact
    24121 cttacttgga aagttacagg tgaagaatat aaaaataatt tacaaatgta taaagattta
    24181 aaggctttag atgatatacc taaatatgaa gatacgattg atacaagctt attggactct
    24241 tgtggagctg atgattttga caaatttata aaagaaaaag ttaacccaga gtttcctgaa
    24301 ggtatgagtt atgaagactg gaaagcaaag gtattataaa taaatggaga atatgaaaaa
    24361 tataataata ctttttatca ttattttggg aagtatattt ataatatttt ttaattttga
    24421 agataaagaa gaaataaaag gaaaattaag aataggggta tctgatgata cctctggttt
    24481 tgtaataaat tatatggtaa atagagacta ctttgaaaat ataaaagtaa gtgatataat
    24541 ggatattttt acaattaatg attgctgagc aagtacaatg cagtgggctc tgagttcaga
    24601 ggaaatagat atagcaatca tatgtaaaga tgctgcaaaa caatatgtaa atgtaaatag
    24661 tggatttgaa attgttggta ctgtagttca aaattctgat atatttctat taagtgataa
    24721 taatcctgaa aagataggag tcattcaaaa tagagattac cagtcagaat tggttaaaaa
    24781 atactacaag aatgtggaag tagctccctt attagggact gcactagcat atggattaga
    24841 aagtaattta gtagatggtg ttgtaataga ttgcataaag tcaattgggt tagagggtaa
    24901 aagaaaatct acaactaaat taggagatta tgatacctat gtattagttg taaataaaga
    24961 gtttaaaaaa agtaaagcat atagtgattt tataacactt tacaataaaa gtgttgatgc
    25021 tttaaagaat aaagctacat ttaagaaagt attaaaagag tacaggaata taagaaatat
    25081 agatatggag gaaataagca attggaaatt aaaatttcta aagataaaag cgatagatta
    25141 actaagaaaa aaactgatgg tagtatggat attttatata aaataatact attagttatt
    25201 atatttttag tctggcattt tgcagcaaaa gatataggga gttctttgtt acttccaatg
    25261 ccagtagatg taataaaagg tttctttttc tgtgtgacag atgcagagac agtcactaat
    25321 ctttttataa cattgcagag agtacttaaa ggttttatgt atgcattatt gtttgggctt
    25381 ccaataggat ttattatggg attttctaaa acttttgaaa gggttttatc tccagtagta
    25441 gattctgtaa ggcaagttcc aataatggca tgggttcctc ttactatagt atggtttggt
    25501 ataggagatg gtccaacaat atttttaata gctttttcag gggttttccc tataatttta
    25561 aatactattc aaggagtaag agcaatatca aaggattatt ataatgcagc tagaagtatg
    25621 ggagcatcgc caattgtgat atttacaaat gtaattgttc ccgcatctct tccagatata
    25681 ctaactggtt caaggattgc tataagtaca ggatggatgt ctgttatctg agccgagttt
    25741 atagctacga gtgctggtct cggttactca atggtacaag ctcaaacatt aatgcataca
    25801 gatgtattaa ttgggcttat gatatttgca gctttaatag gatttataat agatagggta
    25861 ttaaagttta taaataaaat cctttgtaga tggaggtttg cagattaatg aagttaagtg
    25921 taagagatat aaataagaca tttgtaaata atagagtaca tacaaaggtt ttagaagata
    25981 taagtatgga tatagatgat ggacaatttg tctgtctttt aggaccatca ggttgtggaa
    26041 aaacaacttt acttactata ataggtgggt ttcaaaaatc agaacgtgga gatgttttta
    26101 taaatgataa aagagttaaa aaaccgggca tagatagagc ttttatattt caaaattatg
    26161 ctttatttcc ttggaaaact ataagaggta atgttctatt tcctatgaaa cagcagaaaa
    26221 taccaaaaga aaaaagagaa gaaatgctag aagaactttt ggttatgtca gacttgaaag
    26281 gaaaagaaaa actgtttcca catcaattat ctggaggaat gaaacaaaga gtggccatga
    26341 taagagcttt agcatgtaat ccagaggtat tactaatgga tgaaccatta ggagctgtag
    26401 attttcaaat gagacaaaat ttacaggaag agctagaacg aatatggata aagaagaaaa
    26461 taactgcatt aatggtaact catgatgttg atgaagctgt atatatgagt gatagagtta
    26521 tagtaatgtc gagagataaa ggaagaatta ttgatgatat aaatatagat attccaagac
    26581 ctagaattag aggaagccaa aaatatgaag agtacaaaaa caaattaact gatactttat
    26641 caaagtgtta tgaagtttaa agaggagtag acatgagtca aatagaaaaa tacaatagtt
    26701 tggataatac acaacagaaa ataagaatct ctattatgga tatgataata gataaaggtt
    26761 ctagtgttac acttcaagaa gtgactgaat atataagtaa aaagttgaat atagaaaaag
    26821 aatatataga aaggacacta caatatttta tttataaaaa tataatggtt gttgatggaa
    26881 atagtataaa ttttatatat cctgtatcag cattgccaac aaatcataaa gtaactttga
    26941 gagataatag aagtttcagt gcaatgtgtg ctatagatgc tataggaacc tcttgcacat
    27001 tcaaccaaga tataaaaata aactctattt gtagtgctac aggtaaggaa atagaagtaa
    27061 ttataaaaaa tgaaaagatt gagtatgtga ataatccaaa cttaagagtg cttcatataa
    27121 atcttgataa gcatctaaat tgggctgcta gttgctgaaa tataatgaat ttcttctgga
    27181 cgaagagtga ttttgatgct tggacaaacg aagcgggatt atctgatgat gaagatatat
    27241 attgtttaga tattaatgag gcaatagtag aatcatataa aatatttaaa ctcaaacaaa
    27301 aggtactatc atagatatac atatatgaaa aactaaaaat gacctttaga taaaaatgat
    27361 aggtaatatc catactatat aaattagtat agagtaggga tgtgaaaagt atttaaatct
    27421 aaaggtatat ttttagtttt tttagatgat tagatagcct tattaagtat atatttattt
    27481 ataacctttg caactccatt ttcattatta gtatcagtaa cataatctgc aatttccttt
    27541 atactattac gtgcattccc catagccaca ccaagacctg catactttat catatgttgg
    27601 tcattttctt catcgccaac agcaatagca ttttctatag gtatatttag attcgtacat
    27661 aatgttttaa gagcagttcc tttattagta gttttaccta aaaattctag ataaaaagga
    27721 gcagacctta ctatagtata tttattgaat aaatttttag gtagttgctg tatgatttct
    27781 tccaatcttt ctggttcatc aactatcata attttacaga ttttaataga ttcatcaatt
    27841 ttatcttttt gcatatattt gacttctata ttatttaatt tcgcttcatg aagtgtatat
    27901 ttgccaggcg tttcatttgg tgtaattaaa tttgtcattg tatttatgtg tattttagtc
    27961 ttatattttt tacaaacgtt attatatata aaatcaaaat catctaaact catttctaca
    28021 tcatgtatga ttttctcact tttaacttct tgaataattg ctccattaaa gcatataaca
    28081 taatcacctt catctgttaa atttagttga tttagaagtt cttttacacc aggaagaggt
    28141 ctgcctgttg ttataactac ttttgcccca gccctttttg cctcctgaat ggactcaaag
    28201 acttcaggag taatcctttt ttgtgtattt aatattgttc catcaatgtc tagtgcgatt
    28261 aatttgtaca tatcaacctc caataaatta tatactcaaa tttcctgtga aaacgttgac
    28321 acagctagtg ttgaacaaag tttcagtggg aaatatggtt tgttttgtat actattatat
    28381 caagagataa gtaatttaga aagtttaata atattaattt tttgataaaa atattactta
    28441 tgtgagatga gaatgtaaaa actttgtaat caataattcc aaaattctta attacaaata
    28501 atggtatcgt ttgcattgtt aatataaata aatgtatatt atatagttag aagtttgaat
    28561 aaatagactg atggtaattt gttatattag ttagttttgt gttataaaat attgtaattc
    28621 cattaatcat atgttattat tcaacatgat tataatataa agaatatttg atatatataa
    28681 taatttataa aagagtatta catagtacat gtaaatctat atttataaat acaataaagt
    28741 aaagttgtaa aggagtgctt taaatgaagt tttcagagtt taagtatgaa agacccaatt
    28801 ataatagtat gaaaaaagag tttttgagtt gtgtagaaga tataaataat tctcgtaatt
    28861 ataaagagca acaaaaaaat atacataaga ttaatttatt aagaaataaa atagaaacgt
    28921 tgtcaaatat agcgtcgata agatatagta cagacacatt taataaattc tacaaagaag
    28981 aaaaaaatta ttgggatgaa tatatgcctt tgtatgaaga attaaattca catttttata
    29041 atgccattgt aaattcaaaa tttaaatatg atttgataaa agagtttgga gagcaatttt
    29101 tcacaatagt agagtattct ttaaaaagtt tttccaaaga aataataagt gaattgcagg
    29161 aagaaaataa attgtgttca gagtatacta gacttttagc ttcagctgag ataatgtttg
    29221 atgggaaaat aagaaattta tctggtatgg ggaaattcat gtattctaag aatagaaaaa
    29281 caagagaatt agcaaacaaa gcatattata atttctttga agaaaatgaa actaaatttg
    29341 atgacatatt tgataaattg gttaaactta gagataagat gtctaaaaaa ttaggttttg
    29401 aagattttgt agagcttggg tacgttagaa tgatgagaag tgactacaga gaatatatga
    29461 taaaaaatgt tagaaaacaa gtactaaaat atgttgttcc aatggcaaat gaactttatg
    29521 aaaaacaagc taaaagaatt ggtctagaat atttaagtta catagatgaa ggcgtggaat
    29581 ttttaacagg aaatgcaagt ttaaagggag attcaagata cataattcaa aatggtaaaa
    29641 ggatgtattc tgaactgtca aaagaaacaa atgaattttt tgattttatg ttagaaaatg
    29701 aacttatgga tttagaaacg aagaaaggaa aaggagctgg agggtattgt acttatatac
    29761 cagattataa atcaccattc attttttcta attttaatca aactgctgat gatatagatg
    29821 tgttaacaca tgaggcagga catgcatttc agttgtatat gtctagatgg attgatatgc
    29881 cagaaattaa ttttccaacg cttgatagtt gtgaaatcca ttcaatgagt atggaattta
    29941 taacatggcc gtggatggat ttatttttta aagaagatac agataagtac aaattcactc
    30001 acttatcatc ttcaattaaa tttatacctt atggagtaat tgttgatgaa tttcaacact
    30061 atatttacaa aaatcctaat gtagataagt caaaacgaaa agaaatctgg agatttttag
    30121 agaaaaaata cttacctcat agaaaatatg gtgataattc ttttttagag agaggttgtt
    30181 ggtggtttaa acaaggacat atatttaaaa atccgtttta ttatatagat tatgtattag
    30241 ctcaaatttg tgcgttacaa ttttggaaaa aaatgattca agatagagat gcaggatgga
    30301 aagattatat aaatatctgt aaagttggag gaacaaaatc atttttagat attgtgagta
    30361 tgggaaattt gtattctcca tttgatgatg gctgtattga gtctattata ggtgatgtta
    30421 aatcttggtt tgatgaaata aatgacagca aattataaaa aattatgaga attttattgt
    30481 ataaaagtga aaaattaaaa aatatataaa aag
//
LOCUS       DAGSWI010000041        30323 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.041, whole genome shotgun sequence.
ACCESSION   DAGSWI010000041 DAGSWI010000000
VERSION     DAGSWI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.041"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            32..1144
                     /locus_tag="MJF80_001656"
     CDS             32..1144
                     /locus_tag="MJF80_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBZ0280324.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKIKNNKSTLSIILSISKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKEDI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGVLLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLVFSLLYVLFWIIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="MJF80_001657"
     CDS             1398..3422
                     /locus_tag="MJF80_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBZ0280325.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKLIDQISKIAKNGTSKNRVSGADRHETNANVIKN
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="MJF80_001658"
     CDS             3642..4328
                     /locus_tag="MJF80_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBZ0280326.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="MJF80_001659"
     CDS             4423..5478
                     /locus_tag="MJF80_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBZ0280327.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAISKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFRSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7149
                     /gene="murJ"
                     /locus_tag="MJF80_001660"
     CDS             5593..7149
                     /gene="murJ"
                     /locus_tag="MJF80_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBZ0280328.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGNGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7195..8901
                     /locus_tag="MJF80_001661"
     CDS             7195..8901
                     /locus_tag="MJF80_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBZ0280329.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSIIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDECGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8923..9996
                     /locus_tag="MJF80_001662"
     CDS             8923..9996
                     /locus_tag="MJF80_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBZ0280330.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10008..10730
                     /locus_tag="MJF80_001663"
     CDS             10008..10730
                     /locus_tag="MJF80_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBZ0280331.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10746..11903
                     /locus_tag="MJF80_001664"
     CDS             10746..11903
                     /locus_tag="MJF80_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBZ0280332.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11906..12745
                     /locus_tag="MJF80_001665"
     CDS             11906..12745
                     /locus_tag="MJF80_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBZ0280333.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12760..13935
                     /locus_tag="MJF80_001666"
     CDS             12760..13935
                     /locus_tag="MJF80_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBZ0280334.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13964..14713
                     /locus_tag="MJF80_001667"
     CDS             13964..14713
                     /locus_tag="MJF80_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBZ0280335.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14735..15643
                     /locus_tag="MJF80_001668"
     CDS             14735..15643
                     /locus_tag="MJF80_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBZ0280336.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15691..16443
                     /locus_tag="MJF80_001669"
     CDS             15691..16443
                     /locus_tag="MJF80_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBZ0280337.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16397..17764
                     /locus_tag="MJF80_001670"
     CDS             16397..17764
                     /locus_tag="MJF80_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBZ0280338.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            17770..18861
                     /locus_tag="MJF80_001671"
     CDS             17770..18861
                     /locus_tag="MJF80_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBZ0280339.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18945..20858
                     /locus_tag="MJF80_001672"
     CDS             18945..20858
                     /locus_tag="MJF80_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBZ0280340.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFMATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21057..21764
                     /locus_tag="MJF80_001673"
     CDS             21057..21764
                     /locus_tag="MJF80_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBZ0280341.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
     gene            22065..24176
                     /locus_tag="MJF80_001674"
     CDS             22065..24176
                     /locus_tag="MJF80_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBZ0280342.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24374..25339)
                     /locus_tag="MJF80_001675"
     CDS             complement(24374..25339)
                     /locus_tag="MJF80_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBZ0280343.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25624..26580)
                     /locus_tag="MJF80_001676"
     CDS             complement(25624..26580)
                     /locus_tag="MJF80_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBZ0280344.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26948..27736
                     /locus_tag="MJF80_001677"
     CDS             26948..27736
                     /locus_tag="MJF80_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBZ0280345.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27859..28329)
                     /locus_tag="MJF80_001678"
     CDS             complement(27859..28329)
                     /locus_tag="MJF80_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBZ0280346.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28468..29112
                     /gene="uppS"
                     /locus_tag="MJF80_001679"
     CDS             28468..29112
                     /gene="uppS"
                     /locus_tag="MJF80_001679"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBZ0280347.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29155..30060
                     /locus_tag="MJF80_001680"
     CDS             29155..30060
                     /locus_tag="MJF80_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBZ0280348.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTNQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        12021 a         3302 c         5377 g         9623 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaagataaa
      241 aaataataaa tccacactga gtataatact tagtatatcc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attatctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agcttagtat atttactgct
      421 aaaaaaatta aatgttggaa gtactgctac aataattact ttcttatgta tatctacatt
      481 tataatagtt gggtactaca tattagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa aggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaag acataggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtgtat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tgggagtagc cctattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttgtatttt ctttattgta
     1081 tgtactattc tggattatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagac gctaaagtct ttgatttgtt caaatataca gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagagtta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgctgtctg
     1441 tggcaatggt ggcaccaaac gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aatcacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaggatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgacgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtgactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agattctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaacttata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag aatttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gattcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aagacttatg
     2701 tttctgcata ccacaataca aatttatcag aaaaaacagc agaaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcaggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggatctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaataaaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacag ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattga gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggtcctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagagtt gatagaaggg agtataccag aaatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc aatatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 atgaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gcaaaaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agaatttatt gcagagattg attctaattt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctagattag actttggtag cagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaggagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattgggct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaaaata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaacgt atggatacta ccagcaggag cactattggc tatggcgagt
     6181 cagctattgt ttcaactgcc atttgcattt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtctat aaaagaatta gtcaacctag tattacctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gacagattat tggcaaccac tttgggtaat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagtac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttaggatatt attccaaaga
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacgc caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atccaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagataga taattacctt gaaattttaa attagagtca gaaaggatga aaatatggat
     7201 tataagaata attatgagat gtggttaaat tctccttatt ttgatgaaca aacgaaaaat
     7261 gagctattaa gtataaaaga tgatgaaaaa gaaatacaag atagattcta taaaaattta
     7321 gagtttggaa caggtggttt aagaggtata ataggagcgg gaactaatag aatcaatatt
     7381 tatactgtta gacgtgcgac actgggcgtt ttaaattata tcatgaaaac tcaaggagaa
     7441 gaaggtaaac aaaagggaat agttatagca catgatagta gatacatgtc tagggagttt
     7501 tgtatagaag tagcaaagac tttaagtgct tatggtgtaa aggcttatat ttttgaagaa
     7561 ttaaagccta caccagaact ttcttttgct gttagatatt taaagtgtgc tatgggaata
     7621 gtaataacag caagtcataa tcctaaagaa tacaatggat ataaagtata tgactcagat
     7681 ggtggtcaaa tctgtataga tatggcaaat gatataatag cagaagttaa taagattgat
     7741 gattacagta ttattaaaag tatagatttt gaagaagctt tatctaaaaa tttaataact
     7801 atattagata atgaagtaga tgatgagttt ataaaagctg ttaagaagca agttttaaga
     7861 caaaatataa tagatgaatg tgggaaaaaa ttaaaaataa tatatacacc tattcatggt
     7921 acaggaaata agccagtaag aaaagtctta aatgaatgtg gttttgaaaa tgtaatggta
     7981 gttaaggagc aagaattgcc agattctaat ttttcaacag taaagtatcc taatcctgaa
     8041 gaaaagtcag tgtttaatat agctattgaa atggcaaaga ataatggcac tgatttgata
     8101 ataggaacag accctgattg tgatagagta ggtatcgttg taaaagattc aagtggtgaa
     8161 tatgtagtct taaatggaaa tcaagtcggt tcacttttag ttagatatat attagagagt
     8221 ttagttgaag aaaataaact tcctaaaaac aatcctacaa taataaaaac tatagttaca
     8281 tcagaactag gtgcaaaaat agcaaaggct tataatgtag attgtttaaa tactttaaca
     8341 ggatttaaat ttataggaga aaaaataaaa gcgtttgaag aaagtaatga tagaagtttt
     8401 ataatgggat atgaggaaag ctatggttac ttaattggaa ctcatgctag agataaagat
     8461 ggtgttgtat cttcacttat gatatgtgaa atggcagcat actattcttc aaaaggtatg
     8521 aacttgtatg aagctttgat agatacatat aataaatttg gatactacaa agaggactta
     8581 aagtcagtaa ctttaaaagg catagatgga ataaaaaaaa taaaagagat gatgctttac
     8641 tttagaagtg ttaaaataga taatgtggct gatgtaaaag tagataaaat tttagactat
     8701 aaagatggtg ttgatgattt acctaagtca gatgtactta aatttttact tgaagatggc
     8761 tcttggatag ctataagacc ttcaggtaca gaacctaaaa ttaaatttta ctttggagca
     8821 aattcagaca atcaagaaga tgtagaattt aagttgaaca atttaatatc ttatatatta
     8881 aatgtagtgg attctatata aaaagctaag gggagataaa taatgaaagt atacaatgtc
     8941 ataatggctg gaggaggagg aactcgtttc tggccactta gtagacaaga agtacctaaa
     9001 cagcttataa atttaagtgg agaagatgct ttaataaatg aaactataaa cagaatagat
     9061 tctctagcaa aaaaagatga tttatttata gtaaccaatg agaagcaatt ggaagcttta
     9121 aaggatatag taaaagataa atgtttggat agtaatatat tgccagagcc atgtgccaga
     9181 aatacagcag cagctatagg ctttgcagca tttaacataa tgaaaaaata tggtgatggt
     9241 gttatgtgtg tatatccagc tgaccattat ataaaagatg aaaaagaatt taaatctatt
     9301 ttggaaaaag ctatctatat agcagaaaat aatgataaac tagtaactat tgggataact
     9361 cctacatttc catcaacagg atatggatac ataaatttta atagagaaaa tactatagaa
     9421 gatgttgcat atgaggtagt ggagtttgta gaaaaaccaa actatgaaat tgctaaagaa
     9481 tatgtaaact ctaaaaagta tgtatggaat agtggaatgt tcgtatggaa ggtatctaaa
     9541 atactagaag attttaagag atacttgcca aaagtatatg aaaaattaga agatataagt
     9601 aaatacttag gaacaaaaga agaaatggaa aaaataaaag aaatataccc aactatacaa
     9661 tcaatctcta tagattatgg tattatggaa agaagtaatg atgttatagt agttcctggt
     9721 gattttggat ggaatgatgt tggttcttgg gattctctag gtgcaatata tccaactgat
     9781 gatgaaggta atatcaaaag aggtgaaaat attactatag atactaaaaa ttcaattata
     9841 tattcagatg acaaattaat atcgacaata ggtattagtg atttgatagt agtatctaca
     9901 aatgatgcag ttatggtttg tagaaaagat aaagctcaag atgtcaaaaa aatagtagag
     9961 cagttaaaag aagaagatag acaagaatat atgtaggagg tgttcatttg attcctaaaa
    10021 aaatacatta tgtatggttt ggtggtccaa aaggaaatat agaaaatata tgtataaatt
    10081 cttggaaaga aaagcttcca gaatatgaga tagtagagtg gaatgaaaaa aactttgata
    10141 tagagaaaga aataaaagga aataagtttt tggaagaatg ttataaaaga aaactttggg
    10201 cattcatatc tgattataca agaataaaag tactctatga acaaggtgga gtttatatgg
    10261 atacagatat gcaaatatta aaggatataa ctcctctttt agaaaataat aggctaatct
    10321 gtgggtatga agatgataga gagtatataa atggtgctat aataggtgta gaaaaaggac
    10381 atcctttttt aaaagactta ttggagtact atgaaaaaga agtacttact tcttcattgt
    10441 ttacaatacc aaagataatg actcatctta tggaaaagaa ttataagaag attgacccaa
    10501 ataattatga agaaggaata cgtgtttacg acaaagaata tttttatcct tttggattta
    10561 aagaagactt tacaccagaa tgtataacag aaaacacttt tgggatacat tggtggggaa
    10621 aaagttgggc taaaaagaga aattatttct tagaaagcaa acacttaaca ggtgtaaata
    10681 agatatggaa gtgttgtaaa atatttgcaa gtaatacttt acgaagctaa aagggagaaa
    10741 gtaaaatgaa gactttaaat gatgatagaa atattatgat tagaaaatat ctttatatat
    10801 ttgttttgtt tatcatatta cttagaaagt atatgatatc atttattgac ccaaatatgg
    10861 atatagggat gattaaatca gcgttatttt attcttcaat aggaatacta atgctacttt
    10921 ttttatttga taagagaaaa agtataacag aaatggtact agttggagtt tgtgtcttac
    10981 tttatttatt aaatagggaa ggagctatat tattaatcgt tttattagct gtatcagcta
    11041 agcaaataga tgataaattt atagttaaaa attatttaat aatatcagca tgttttttaa
    11101 tggtagctat attattattt aatctatttc cttcattaat atttaatcag gaagtccact
    11161 atagatatat agaaaaaata gatatgcttg ttacaagaat ggattttgga cttggtaatc
    11221 caaatagtgt attttatcat atggttacta tatatgcagc atatatattc ttaagatata
    11281 aagattataa taaatgggat agaataattt tatttggttc tgcatttttt gtttatcaaa
    11341 ccacatatag tagaactgga tttttcacta tattagcagg attaatattt gtagaaataa
    11401 taagatggat agatataaaa aaaataaaag gactaccaat gctcctaaag acattaccaa
    11461 taatacttac tttatttagt gtaattattg gtacagtatt tgataaaagc actttattaa
    11521 atagattatt agcaagtaga cctaagttct ggcatgtata cttggctgaa aaggggaatt
    11581 ttctaaaacc ttttggaaat tcatatagtc ctgctataaa agcaactaat ccattagata
    11641 gttcttatgt atatataatt agtgtactag gagtagttgc ttgtatatta tttatgtatt
    11701 tgatgtataa aggaatagaa agctttatag aaaaagataa aaaagcatat ttagtagctt
    11761 tatttatatt tttactatac tcttttgcag aaaatttatt attagaagct tcatttagtt
    11821 ttgcagtagt attactaata aaagaagtga tattaaatga taaaagggaa atagatttgt
    11881 ggaagatgaa aagtaggagg tagtaatgtt aatatcatta ataatgccaa cattaaatag
    11941 atatgatgat atatacttac ttatggacag cttagaaaat caaacttaca aaaactttga
    12001 acttatagta gtagaccaaa atgataatag caaagttaaa gaaatagttg ataaatacat
    12061 tgataagtta gatataaagt atataaaaag ctctaaaaaa ggattgagtt ataacagaaa
    12121 tgtaggcata gacaatgctg tagggcaaat tataggtttc cctgatgatg attgtgtcta
    12181 cgaaaatgat acattagaaa aagttataaa tttctttaat aaaaataaag attataaaat
    12241 atacagttgc aaaactatgg attcaaataa agttgatgca tttaagaaaa tgtatgatgg
    12301 aacttgtgat atcacaagtt ccaacgtttt ggatactata acatccataa ctttttttat
    12361 tgattttgaa ggtaaagact atacaagatt tgatgagaaa ttgggtgttg gtggagagtt
    12421 tggtgctggt gaagagatag attatgtttt aaacttattg agtttaggtt ttaaaggaaa
    12481 atactttgga aatgatataa tttaccatcc tgcaaaaaaa cattctaaat ctaaagaaaa
    12541 gtatcaaaaa gattataact atggaagagg ctttggagcg ctttgcaaaa aggaaatagt
    12601 ctatagaaaa aattataagt ttgcaaaagt tatggtatca aaacttgtaa gaaatatagg
    12661 aggtcttata ttgagttcca atagagatta tcatagtgcc actataaaag gaagaattaa
    12721 tggatttaga cagtacaagt tataatgagg atggtgttta tggacaaggt taaattttta
    12781 ataaatatgc ttttggcttt ttttatatat ccatttaata agcataagtt taatggtaga
    12841 gatatctggc ttataggtgg acattcaggg gatatataca atgataattc aaagtttttt
    12901 tatgagtata tgctaaaaga acataatgat gtagaaactt attgggtagt aaataaagac
    12961 agtaaagtat ttgataaaat tccaggcaaa aaattaataa gaggaagtgt agaaaactat
    13021 ctgtattatt acaattcaaa agctatagta ttttcacatg caccatcagc agatatagca
    13081 ccatacaatt ttgctgtacc tgttcttaat tattttcata aaaaaacgat aaaagtgttt
    13141 ttaaatcatg ggactataag ttttaaaaaa agaaaaccta tgaacaaaaa gttcaaaaat
    13201 attatagata acctatataa aagctataat atagttacag caagttctga atttgaaaga
    13261 aatgttatgg taaatgattg gggaatgtta gatgactcag tttatataat tggaaatgca
    13321 agatatgaca atttaccaac aaatgaagtt gcacaaactc gagatatatt atatactcct
    13381 acatggagag attggataaa atttagttca ggaaaattta cggatacaga ttattttaaa
    13441 aatataatga attttttaaa tgatgacaaa ttaaataaaa tattagatga gaaagatata
    13501 aatgtaaaaa tttatatgca tcatcttatg catgagttta tagatgatat aaaagaaaat
    13561 ataacaggaa aacgaattgt gtttttagat aaaggggtaa ctctagcaaa tgaaatcaga
    13621 aaatcagctg caaatataac tgattattca agtgtagcca tagacttttt gtatatgaat
    13681 agacctatct tgttttacca attcgatttg gatgaatata tggaaaaagt tgattcatat
    13741 atagatttaa aaagtgaaat gtttgggtct ttggcttata ataatgatga agctgtgaat
    13801 aaacttattg atattattga aaataatttt gaggttatgg ataaccaaaa aaatgaaaga
    13861 aataagtttt tcagatataa tgataataag aactgcaaga ggatatatga ttgtgtatta
    13921 agtaaaatta aataaatatt ttataaatga tgaggaaggt aatatgaaga agaatttagt
    13981 atctataatt actcccatgt ataattctga aaaatttatt gaagcaacca taaaatcagt
    14041 attaaaccaa acttatcaag aatgggaaat gttaattatt gatgattgct caacagataa
    14101 tagtcctaat atagtcaaat cttatatgca acaggatagt agaataaaat gtataaagac
    14161 tgagactaat aagggtgtct ctaatgctag aaatttagca ctaagtaagg caacaggaca
    14221 atttatagct tttttagata gtgatgacca atggaatagt agtaagttag aaaaacaagt
    14281 aaattttatg ttagaaaatg actatgtaat ttcatttact tcatatgaac tgatggatga
    14341 aaatgataaa aaattaaaca aagtaataaa agttccacct aatgtagatt atagaagatt
    14401 attaaaagga aatatcttag gatgtcttac tgttgtgatt gataagtcta aattggattt
    14461 tgaaataaga atgagtggtg taagacatga agattatgtt ttatggttat cgatattgaa
    14521 aaaagggcat attgctcatg gaataaatga agttctagca ttatatagaa aatctagcaa
    14581 ttccctaagt ggtaataaaa taaaagctgc tatgtggact tggaatatat atagaaatat
    14641 agaaaaaata cctttataca aagctatata ctactttatt aattatggaa taaacggaat
    14701 caaaaaaagt tagacagaga ggaagattta attaatgtat aaatataaat ttactgtatt
    14761 tactccgacg tataacagag ctcatttact tgaaaatttg tacaatgatt taaaagctca
    14821 aacttatgat ttcaatgatt ttgaatggtt gattgttgat gatggaagta gcgacagtac
    14881 taaagagtta gttgaaaaat ttatatcaga aaataaatta aatataagat atatatacaa
    14941 agaaaatgga ggaaaacata ctgcaataaa tctaggtgta aaaaatgcag atggagagct
    15001 atttttcatt gtagatagtg atgatggttt aattaaagat tctcttgaaa ttgctaatga
    15061 tgagtgggat tctttagaga ataaagaagg atttagtgga gttgttggac tttgtgtata
    15121 tccaagtgga gatttgatag ggacagaaat gcctgaagat aagaaaatat gtcactttgc
    15181 agacctatat tttaagtatg gcgttaaggg agataaaagc atagtatttg taactgatga
    15241 gcttataaaa tttccttttc cagaaagaaa agaagtgaga tttttaccag aaagtgtagt
    15301 atggaatgaa atgtctaaat actataaagt tatatgtgta aataaaccta tgataataag
    15361 agattatttg gatgatggtt tgactaagaa tatattaagt aaaaatgcat taagaggtag
    15421 agcattagag tttttatatt taataaatca aaatacatat ccattaagta gatatccata
    15481 tatgtggata aaaaattaca taaatttagc cagatactca ttgttgtctg atagtcatta
    15541 ttttggtgaa ttaagaaaag tatctgataa attgttgtat ttagcattat tcccattagg
    15601 atattacaaa tatattggac aaagaaaatt agtatctaag taaacttgtt ggtatagtac
    15661 tgctagttgc tatataagga gagtaaaaat atgaatgagc ctttagtatc gataattact
    15721 cctgtttata attcagaaga gtttttatca gagacgataa agtcaatcca aaatcaaact
    15781 tataaaaatt ggcagctttt attagtggat gattgttcta aggacaattc aagtgaaatt
    15841 ataaaaagtt ttcgaaaaga agatgctaga ataaaatata taaagcttga aaaaaattct
    15901 ggagctgcag tatctagaaa tgttggtatt aagaatgcgg aaggtcgatt tattgcattt
    15961 gtagatagtg atgatttatg ggatagtaga aaattagaga ttcagataga atatatgtta
    16021 aaagaaaatg taggtttttc ttttacatct tatagatata tgagacaaga tgggtcaaaa
    16081 acgaataagg ttgcaagagc tcctaagaaa attgactatg aaggattact tagaaatact
    16141 ataataggat gttctacagt ggtcattgat aaggagattg taggagagtt cagcatgcct
    16201 ttggtaagaa gaggacaaga tactgcaacc tggttacagt tacttaaaaa ggaaaaatat
    16261 gcatatggga ttcaagaaga tttagtgaat tacagattag ttggaaattc tatatcaagt
    16321 aacaagataa aagcattaaa acgaacttgg aatacttata gaaatgttga aaatttaagt
    16381 ttaccaaaaa gtctatatgt attttgtttc tatgttttta atgccattaa aaaaagagta
    16441 taagttttat atacttggag gtaaaaaaat gaaaatagct atagctggga cgggatatgt
    16501 aggtttggtt acaggtgttt gtctagcaga ggtaggcaat gaaaatgtag tctgtgttga
    16561 tgtagatgaa caaaaagtta aatcaatgaa acttggaata gcacctatat atgaagaagg
    16621 cctagaagaa cttatggtta aaaattacaa cagaggaaga atagattaca ctacagacta
    16681 taaaaaagct tatgaagatg tagatgtaat attaatagct gtaggtacac ctgagagaag
    16741 tgatggctca gccaacctag attatataaa ggcagttgca aagcaaatag ctgaaactgt
    16801 gacaaaagat actctagtag taataaaatc tacagttcca attggtacaa atgaagagat
    16861 agaagattat ataaatagaa acctaaaaaa tgatgtaaaa gtagagcttg catctaatcc
    16921 agagttttta tcacaaggaa cagcagtaaa agatactcta catgcttcaa gaatcgttat
    16981 aggtgtagaa agtgaatggg ctagagaaat attagaggaa atctataagc cttttaatca
    17041 acctataata gttacaaata gaaatagtgc tgagatgata aaatacgcat caaataactt
    17101 cttagctctt aaaatatcat ttatgaatga tattgcaaat ctttgtgaaa tagcaggagc
    17161 taacatagaa gatgtagctt taggaatgag ttatgatgat agaataggaa gtaagttttt
    17221 aaaggcggga ataggatatg gtggttcttg ctttccaaaa gacacaaaag ctcttcactg
    17281 gttatctgaa cataatggtt atgaattaaa aacagtaaaa gctgcaatag aagttaatga
    17341 aagtcaaaaa cttaaattag ttagaaaggc atctaagtta gtagagagct ttcaagatat
    17401 gaaggttgct gttttaggtt tgacttttaa gccagacact gatgatataa gagaagcacc
    17461 atctatacca aatataaaat atttgctcga aagaggggca agagttactg tatacgaccc
    17521 aattggtgta aataatacta agaaaatatt tggtgactct atactttatg ctgattctgt
    17581 agatgatgcg atagaagatg cagagatatg ttttataatg acagaatgga aagatgtatt
    17641 aaattatgat atacataagt ataaggaaaa gatgaaaaat cctttagtcc ttgatggaag
    17701 aaattgttat aaaattaaag atatggaaga gttaggaatt gagtattatt cgataggaag
    17761 gtaaatatta tgaagactgt ttgttattta gctgatgggt caaatcctca tactttaaaa
    17821 tggtgtagtt tttttaaaaa taaaggatat gacattcatg taatctcttt aaatggaggt
    17881 cacatggaag gaataaaaat ttataatttt ggttctaatg tagaagaact aaaaaatgaa
    17941 aatatttcta aaaaaacggg atacttaggt tcaataaagc agataaagaa gctagtaaat
    18001 gaaataaagc cagatatatt gcatgcacag tatgcaagta gttatggatt tataggaagt
    18061 ctacttgggt atcatccata tattatttct gtttggggta ctgatattta tgatttccct
    18121 aataatggtt ttatacaaaa acaaattata aagcataact ttagaaaagc agactatatc
    18181 ttttcaaata gtagagatat ggctaaagag gctaataaat atacagctaa acatgtagat
    18241 gtaacctttt ttggagtaga tatggatagg tttaagccta tggaagtaga aaaagaagac
    18301 gcttttgtaa taggaattat aaagagttta gaaaaaaaat atggaataga atatttaata
    18361 caagcattta agatgcttaa agatgaatat aaagataaga aaataattct taaaataggt
    18421 ggctctggaa gtcagatgga caacctaata aatttagcta aagaattggg tatagaaaat
    18481 gatgttcagt ttttaggaag aatatctcca gaaaatgtaa gcaagacttt taattctttt
    18541 gatgtaactg tatttccttc tttaagagag ggatttggag tggcagctat agaatcagaa
    18601 gcatgtgaag tacctgttat agttacaaat gtaggaggac atccagaatc tgtatgggaa
    18661 aatgaaactg gcttaattgt agaaccaaaa caaccagaag aaataaaaaa tgctataata
    18721 aaactaatgg aaaatgatga acttagactt aatatgggta agaaagggcg tcaatttgtt
    18781 agagaaaatt atgaggttaa tttaaacttt aatgatatag aaaaaattta tgattctata
    18841 tttgataaat ataaaaaata aaatgaaaaa atacagcaaa agatagatgt tgttggctat
    18901 gcttaggtgc gtagcttttg ctgtttaaag gagggcttct agttatgcga gaaagtatag
    18961 atttagagaa agaaagcttt tttaataggc tttttaaaaa atataaattt agacatttaa
    19021 cactattaat tctagatatt tgtagcgtac tagtagcgtt tcgaatgtct ttaagcttga
    19081 caggtaattt aaatgcattt accactaatg atataataat ttcagcgtgt atatatatag
    19141 tacttcatat agtatcattt agattgttta agtgctacaa cacattatgg agatatgcag
    19201 gagaagaaga aattatatct atttttatgg caactttagc ttatttaatt cctatttata
    19261 taataaataa attattaggg tttgattatc ctataatgtt ttatgtatta aatacaatat
    19321 ttattataat gtttacaagt ggtgcaagaa tagcctatag agctataaga atagtcatga
    19381 acaagacata ttctagagga aaggtttcaa atatccttat aattggtgca ggagatgcag
    19441 gagaaatggt tatacaggaa ctaaaaagaa atccagaact taagaaagta gcagtggcta
    19501 taattgatga tgataaaaat aaaattggaa gaataatcca taatgtgaaa attgtaggaa
    19561 caacttcaga tataaaagat gtggtagaaa agtacaatgt agatgagata atcttttcaa
    19621 tagcaaacat agagaaacgt agaaaaaaag agattattga tatatgtaaa aacactaact
    19681 gtaaaataaa gacaatacca ggaatttacg aaattataga tggaaaagtt gatataaaac
    19741 agattagaga agtagagata gaagacttgt tgggtagaga gcctataaaa acaaatttaa
    19801 gagagataag taattacatt gagggaaaag taatcctaat aactggagga ggaggctcaa
    19861 ttggttcaga gctttgtaga cagattgcag gatttaatcc taaagagctt attatagttg
    19921 ataattatga aaataatgct tactcaatac aacaagaatt acttagaaaa tataaaaata
    19981 aattagattt aaagacagta atagcatcaa taagagaaga aaaaagaatg gatgaaatat
    20041 ttaataaata taaaccagaa gtggtatttc atgcagcagc acataaacat gttcctctta
    20101 tggaatcgag ccctggcgaa gctataaaga ataatatatt tggaacttta aacatagctg
    20161 gcttgtcttc aaaatataga gctaagaaat ttgtccttat atctacagat aaggctgtaa
    20221 accctactaa cataatggga gctactaaaa gagctgctga aatgattata cagactatga
    20281 atgctgaaag ccagactgaa tttgttgctg ttagatttgg aaatgtgctt ggtagtaatg
    20341 gtagtgtaat tccacttttt aagaagcaaa ttgaagatgg cggaccagtg acagttactc
    20401 atccagatat tattagatat tttatgacaa taccagaagc agtaggctta gtcatacaag
    20461 ctggggctat ggctaaaggt ggagaaatat ttgtacttga tatgggagaa cctgtaagaa
    20521 tattggattt agcaaaaaac ttaattaaat tcagtggatt tgagccagat gttgatataa
    20581 aaattgaatt ttctggtctt agaccaggag aaaagttata tgaagaacta cttatgtcag
    20641 aagaaggtct gttggataca gagcataaaa agatttttat tggtagacca atagatgttg
    20701 atagagaaaa aataacaaaa tacttaaaac ttttaagaga aattactaac aatgaagagg
    20761 ttgaaaaaat agatggtatt atgagggaat tagtgccaac ttatattaaa ccagaagatg
    20821 ctaatatcaa agaaattgca acaactagag aaaagtagaa tatatttcta atttgtaacc
    20881 agggggagca aaaagaaaca aatatatact aaaaagaaac aaaagattac aaaaagatta
    20941 caaaaagatt acaaaaaagg ttgcaattta ttttgatgta agatagaatg ttaattagga
    21001 attggaaacg aaggaacaaa atgttaaatt ttcgaaagga ttaggtattt gagattatga
    21061 tgaatattaa aaataaaaaa catatattga aaaaatttat agctatggtg ttaatagcag
    21121 gagttgtcac agtagaagca ggagcaataa cggctagtgc agcagagcca actaattcac
    21181 ctatgagtgc aacagtagac cagtgtgatt ttttaaatgt aagaagtgga gctagtgcaa
    21241 atgatgcagt agtaggaaag ataaacacag gagacaaagt tgaagtttta gaattacatt
    21301 caaatggatg gattaaaata aaaagtgttg acaatgttac aggatgggta aatggagatt
    21361 atttaactat tcaaggtgga aatgtagatg caaaggtaca aaatgtttta aacttagcat
    21421 ttaagcaaca aggtaagcca tataaatggg gagcaactgg accaaattct tttgattgtt
    21481 ctgggttcac ttcatatgtc tataaaaatg gagcaggagt taatttacca agagtatcaa
    21541 gatcacaagc aacagtagga aagaaagtat caagagcaga attaaaacca ggagatttag
    21601 ttttctttgg ttcaggtggt tcaataaatc acgttggatt gtatgtagga gattctaaat
    21661 tcatacattc accacaaaca ggagatgtag taaaagttac tagcatggct ccaggaacta
    21721 attatgcaaa aagacttata actgctacac gtgttttaca ataataatag atattgattt
    21781 acataggatt gaatattaac attaaatata taattacatt taatgtttta catagagatt
    21841 taagagttta gtaatattaa aatagtgatg aacgttataa aaacagcaaa gaagataaat
    21901 ttctttgctg tttttttata ataaattatt tcatattata atcaaatatc aaatataaat
    21961 tttaactata ttattcaata tttgacagat aatatgaatt ttagatatac ttaataagaa
    22021 gtatattgta gaaaaaataa atttaaaaaa ttaaggggga tgaaatgaaa aaaataagta
    22081 tattagtatt atcattgata atgacactta ctatgtgtag tgtaagttca tttgctgatt
    22141 catcaaatga taaggaaatg agagctgctt ggatatctac agtttataat ttagactggc
    22201 ctaagactaa gaataatgaa gccaagcaaa aaaaggaata cacagattta ttggacaaat
    22261 taaaaagtgt aggaataaat acagcagtgg ttcaagtaag accaaaatca gatgctttat
    22321 ataaatcaaa tataaaccca tggtctgaat atcttaccgg tacacaaggt aaagaccctg
    22381 gatatgaccc attaccattt ttaatagaag aagcacataa aagaggaatg gaatttcatg
    22441 catggtttaa tccatataga ataactatgg cagatgagag catagataaa ttgccagcaa
    22501 accatccagc aaagaaaaat ccaagctggg tagttaaaca tggaaataag tattactacg
    22561 acccaggttt accagaagtt agaaagtaca tagtggatag tatagcagaa gtagttcaaa
    22621 attacgatat agatggagtt cattttgatg attacttcta tccaggagta agtttcaatg
    22681 atactgcaac ataccaaaaa tatggaaaag gacaaaataa ggataattgg agaagagaaa
    22741 atgtaaatac tctacttaga gatgtaaaag cgtctataaa gagtataaag cctaatgttg
    22801 tatttggtgt aagcccagca ggtatatgga gaaataagag tagtgaccca acaggttcag
    22861 atacatcagg taatgaaagt tatgtaggta catatgcaga tacaagagca tggataaaac
    22921 aaggattgat tgactatgta gttccacaac tttactggcc aattggtctt aaagcagcag
    22981 attattctaa attagtggca tggtgggcta atgaagtaaa aggaacaaat gtagacttat
    23041 acataggaca aggtatatat aagcaaggtc aatcgagtta tggtggacaa aatatagcaa
    23101 aagaaatagt acaacaagta acattaaata gaaagtatag tgaaataaaa ggtagtatgt
    23161 atttctcagc taaagatata gcaaatagta cttctataca aaaggattta aagagtttat
    23221 attctagtag tgaagaacca gttactccac catctaatgt taaagtggag aaattaagag
    23281 gcgacgaaag atatgacaca gcagtagcaa taagtaaaaa aggttgggct acaaattcag
    23341 atacagtagt attagtaaat gggtactcta tagttgatgg tataacatca acaccacttg
    23401 ctacatcaaa tgatgctcct atactattgg taaataagga taatatacct acatccacta
    23461 agaatgagtt aaaaagactt aatccaagta aagttatttt aattggagga aataactcta
    23521 ttggagataa agttgagagt gagataaaag atacattgtc aaatgtatca ataaatagag
    23581 ttggtggttc agatagatat agtacatctt taatgatagc aaaagaatta gtaaaaacta
    23641 atccagtaga aaaactatat ataacaagtg gtacaggtga agcagattct ctttctatag
    23701 cttcaaaagc aggagaagaa aagcaaccaa tagtattagt atcaaaagat aatgtaagtg
    23761 atgaagttta caattggata tcagatttaa aagtaaaaga tgcttatttt ataggtggta
    23821 acctaagtat atctgattca gtaataaata agctggataa ggtcattaca aatgatgtat
    23881 ctaaaaatag aatagctgga gaaaatagag aagaaacaaa tggaaaagta atacaaaaat
    23941 tctatccaaa tgctgaatat tcatcaatgt ttgtaagtaa aagtaatcaa ttggtagatg
    24001 cattaacatc aggtccttta gcagctaaat taaaatcacc agtagttatg cttggaaata
    24061 gtgtaacatc tgctcagaaa actgctttag agcctaagaa aactactcta gtatacgaag
    24121 ctggagatgg tataaatcaa aacacattaa acactttctt aaacttagtt aagtaataaa
    24181 aaagttaatt taaaatacag agtattttat aaatgtagat taaaatgaaa attatcatga
    24241 taaattaaaa aataaaaaag aactatatgc attttgtaat caagtgtaca cagcaacttg
    24301 aaaataatta tgcgatatag ttctttttta gtaattaatc ttatactttt taataaactt
    24361 tatattgaat tttttattgt ttaaactcta tgtcattaaa tataaaatcg tgtaatatgt
    24421 ctaaagtatc tgggtcaaat ctaagaaccc atccagcttt tccatatata ccacctgtac
    24481 tatgaatttc atcatcaata ggaaattcaa attgcttaat atttaagtcg cccattttta
    24541 aaacttgagc tcctaaagaa agtattgcat taggcttcat gttagtttta acatatggaa
    24601 gaactgcatc taataaacct ggatatttag taacaggtaa agtttccgtt ttcttgatta
    24661 tagcttgtat aatctgtctc tgtctgccat ctctagcaaa ggctgtatcg ttgtgtctga
    24721 tacgtgcaaa agatagtgcc tgatatccat ttagagtctg ttttccagag tttctaatgt
    24781 attgtatgct acctttatca tcacttttat tccatttata agtttcagga ataaatttat
    24841 ttagttctcg tatttctcct ttttcaacag taacctcaac gccaccaata gcatcaatta
    24901 tatacataaa tgattcgaaa ttaacacaag cataattttg aatatcaata ttaaaattct
    24961 cttcaattgt ttgtattaat aaatcagctt gtccataagc ataagcatga gtaagttttt
    25021 gtttaccatg accagggatg tctacatagc tatcacgagc taatgatgta agtttgatat
    25081 cattgtgttt gttatcaata gtaagtatca tcatagcatc tgaccttgaa gaatcttcat
    25141 ttggtctagc atctgttccc ataagtagta tgttaattat cccatcttca tttttatggt
    25201 cattgttatc caataaatca ctacttatgc tcgagtcatg aatagcagat aatttataat
    25261 aaaaatatcc atagacactt atagggaata ttactaccaa gaaagccagt agtataacaa
    25321 atttctttaa ttttgacaaa agtacccctt ctttcttctt tttcctgtat attttttaat
    25381 agcatacact taattatatt aaagtattga gatatattca atacttgttt cagttacaaa
    25441 aaaaagacaa tgatataatt tataagaaaa tttatcgacc tatatatata gttaaaagta
    25501 tatataatat attttttaaa agtaatttat attttaaaat aaaaacacac cccataaata
    25561 caaagtaatt tgcatttaag aggtatgtat ttatcatcaa attattggtt tgttttggaa
    25621 tttctaatct tcaaccataa tatctttaaa tatgaaatca tgcattgcat ctagttcata
    25681 ttcctcaaaa ggtattacat agcctgcatt acctattttt ctaccatttt cagtagaaag
    25741 aggaaactcc atagatttca aatttaaatt tcctatactt aaaagttctt ttcctaaaga
    25801 tattatttca ttaggattca tattagtttt tacatacgga agtattgtgt taactagatt
    25861 aggatattta gtgactggta aatccttaac accatttatt aaactctgta taacacttct
    25921 ttgtcttcta tctctttcat ctgtactatc attttttctt atacgagaaa atgcaagtgt
    25981 ctgatatcca tttaattttt gctcaccaga atttcttata tatttaagag aacctttatt
    26041 gtcattttta ttccatttat aagtttcagg aataaattta tttaattctc ttatttcact
    26101 cttatcaaca gtaacagtaa ctccacctaa agcatcaact atatccataa aagagaaaaa
    26161 gtcaactacg gcgtagtttt gtatatcaag ttggaagttt tcttctatag tttctattaa
    26221 taagttagct tgtccatata cgtaggcatg tgttaatttt tgttctccac gtcctggtat
    26281 attaacataa gtatctctat ttaatgaagt tagcttaaga cttttatttt tattatcaat
    26341 agtaagtatc atcatggcat cagaacgaga aacttcttct cctggtcttg catctgtacc
    26401 taacaataat atgtttgtga tattttcttc gtttttatag tcattactac taagcgcctc
    26461 tgtatttata ctatcatctt gtatagtttt taatttagta aaaacatatc catatatact
    26521 aacaggaaat gctataacta aaagggctag aaatataacg aactttttga gtcctgacaa
    26581 taatatcccc tactttctaa attttttaat tacacatagt taattatatt aaaaatttgt
    26641 tacataagca ataaaaaaca caataaatta gcataagttt gtcataatat aattattttt
    26701 atagcatttt atagaagaaa gtgtgtagtt attattgatt ttattggaat ataattgtat
    26761 gaagacttaa atttttataa actgatgtgt atattgaaaa aaatattttc ctgaatgtag
    26821 ttatagcatc ttatgaaagg tgcaaattgt aatttaaaat ttttagtgta taataagaat
    26881 tgaaaattca aaccgataaa aattaataaa aaataattat aaattaataa tttgggggag
    26941 gaaatgtatg ataaaattaa tagcaacaga tttagatgga actttgttag atgaaaagag
    27001 cgaaataaat ccagagtttt ataaagtatt taaaaaatta agagagagag ggataatgtt
    27061 ttctgctgct agtggcaggc agtatcaaaa tcttataaaa aaatttgaag atataaaaga
    27121 tgatatgatg tttatatcag aaaatggaac actagtagta tataaaggaa aagaaatatt
    27181 atcaaatcct ttaaataaag aattagtaaa tgaaatcata gagacaacaa gaagtataaa
    27241 agggaaaaaa attgtaatga gtgggaaaaa atacgcttat atagaaagca aagatgaagc
    27301 gtttatacaa gaggtcagta cttattatgc aaagtttaaa attgtagagg atttgacgaa
    27361 agtagaagga gacatattga agatagctgt atttgacttc aaaggtgcag aacataataa
    27421 taatatctac tttgaaaagt tttctgatag agctcaagta tgtatttctg gagtggagtg
    27481 gcttgattta actgcaaaag gtgcaaataa agggagtgcc ataaaaaaag tacaaaaaat
    27541 gttagatata aaatatgaag aaactatggt atttggtgac cagttaaatg atgtggaaat
    27601 gatgaaaagt gcctatcata gttatgccat ggaaaatgca aatgaacatt tgaaacaaat
    27661 tgctagattt agagcaaaaa gaaatactga aaatggcgtt gtagataaga taaaagaggt
    27721 tataaaaatt ggatagtcat atgtatagat gaaaaagatt attttgaaga aatcaaaaaa
    27781 atatcaatgt aattttgata aataagttta tttatgcgaa aattacattg atatttaata
    27841 aacatcaata atagatattt atttttttat gatattcagt attttgtttt ctatttgagt
    27901 tgcattttca gcagaaatta aataaacata tctacctgcc ttctcaatta tagtattatt
    27961 tgctatttca gaattgattt tttctccatg tccttctcca aaaggtgctt gtatcatatc
    28021 ttttttataa ttttctagag ttttatagat tttatctacc tcgtcagttt ttataacgat
    28081 aatatcttct agagataatt tttcagaaga attaattaca aaaccttgaa ttatgtcttc
    28141 ctctacatca ttaaaatagt taaaatcctt tgcatcaaag atttttgatt cctttataag
    28201 taaatcagaa gattttaatg ctttttttat ttttgatgta gaaacgtttt tgtaatcacc
    28261 acttgaagaa caactaacca ttcctaatga taagattatc gaaaaaaaca atattaaaat
    28321 ttttttcatg ataaacctcc aagtttttta aactctgtct taaattagtt tactacataa
    28381 ttatataaaa ataaatgtat ttaatgttac agttttgatt aataatgtaa tattttgata
    28441 ataataccta ttgagaggtg attagaaatg agaataccaa aacatgtagg aattataccg
    28501 gatggaaata gaaggtgggc aagtcaaaat ggtatgacaa aagataaagg atatgaaagt
    28561 ggaatagatc ctggactaga gtcttataga ttatgtagag atttaggctg tgaagaggtg
    28621 actttttatg gatttacaac tgataataca agacgtccat cagtgcagac aaaagctttt
    28681 acaaaagcat gtattgatgc tgcaaatata ttaatagatg aaggtgctgc tttattagtt
    28741 gtaggaaata caaaatcgcc aatgtttcca gaagaattat taccatatac taatagaatt
    28801 aaagccaaag aaggtcaaat aaaagcaaat cttctggtaa attatggttg gtattgggac
    28861 ttaaataata tatcaggagt agaaaatgta aataaaaata atatacagga ttacatacag
    28921 tcatttgatg tatccagact agacttgata atcagatggg gtggaagaag gagattaagt
    28981 ggtttccttc ctgtacaatc aatttatgca gatttttatg ttatagataa ttattggcca
    29041 gattttaaga gtgacgatat aaaggacgca tttagttggt atgatacaca agatgtcact
    29101 ttaggtggat aattaaaaat attctgattt aaggttgtag agtgttttaa attattgaaa
    29161 aaaaaacttc ttgatggtaa aataacacta ataatatgta aaagtgtaaa aatttatacc
    29221 aagaagggtg aagaaatgtc aaaaggcaat aataacaata attcaagaaa caaatctaaa
    29281 aagaccagtc atctgaatag aaaaaagcga aaattaaata agaaaaagtt agcagtgttg
    29341 atttgcttta cagttttatt tttatttatt gcatttaagg ctactcaagg tgtagtagct
    29401 ttggtaaaaa gtatggataa aagtaataaa actaatcaac aacaaaatgt taactcagaa
    29461 caatttgacc ttggtaatga agaggaaaat aaaaagaaaa aatatactgt atttatagac
    29521 ccaggtcatg gaggcaatga taaaggaact gaaagcaaga ccagtaatag atatgaaaaa
    29581 gacctaaatc tacaaattgc aaaaaaatta gcaaataaat tatctaaaca aaaggatatt
    29641 caagtagtag tatccagaac tgatgataca tatatatcat taaaggatag agctatctta
    29701 gcaaataata gttctgctga tgtgcttgtt tctattcatt taaatgcaga gaaaaatggt
    29761 aatacagcaa caggtataga aacttggtat aggaataaag ctacagatgg ttcaaaagaa
    29821 cttgcacaga cagtacaaag tactatagta tcctatgtta aagttagaga taggggaata
    29881 gttgaaaata actttgaagt acttagagaa tcaaatatgc ctgctatatt aatagagtgt
    29941 ggttttttaa caacaccttc agaggaacaa aaaataataa atgaaaaata tcaagaccaa
    30001 cttgcggaag gaattgttca aggtgtactt tcatacctag attctaaagg gaataagtaa
    30061 gttaacttta ctttaatgtg aggataaatt taaaagttta actgtgtaaa ttgtattaac
    30121 tttatttcgt aatgttgcaa acattgtttg aatttagaaa ctatgaaatt agaaattatt
    30181 ttatctatat ctaatttcat agtttttttt gcaaaaaatt tatagttata aaattttaaa
    30241 aaataaattt agtataatgt aatgtagtta tttatatgtg attttaactt gcattgattt
    30301 gctatcaatt gtgctttata att
//
LOCUS       DAGSWI010000042        30236 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.042, whole genome shotgun sequence.
ACCESSION   DAGSWI010000042 DAGSWI010000000
VERSION     DAGSWI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.042"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            91..1014
                     /locus_tag="MJF80_001681"
     CDS             91..1014
                     /locus_tag="MJF80_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBZ0280349.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(1186..2328)
                     /locus_tag="MJF80_001682"
     CDS             complement(1186..2328)
                     /locus_tag="MJF80_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBZ0280350.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELRINK"
     gene            complement(2346..4100)
                     /locus_tag="MJF80_001683"
     CDS             complement(2346..4100)
                     /locus_tag="MJF80_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBZ0280351.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            4236..4442
                     /locus_tag="MJF80_001684"
     CDS             4236..4442
                     /locus_tag="MJF80_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280352.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(4447..5370)
                     /locus_tag="MJF80_001685"
     CDS             complement(4447..5370)
                     /locus_tag="MJF80_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280353.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(5624..7324)
                     /locus_tag="MJF80_001686"
     CDS             complement(5624..7324)
                     /locus_tag="MJF80_001686"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBZ0280354.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(7786..8199)
                     /locus_tag="MJF80_001687"
     CDS             complement(7786..8199)
                     /locus_tag="MJF80_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBZ0280355.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(8290..10668)
                     /locus_tag="MJF80_001688"
     CDS             complement(8290..10668)
                     /locus_tag="MJF80_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBZ0280356.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            10987..12438
                     /locus_tag="MJF80_001689"
     CDS             10987..12438
                     /locus_tag="MJF80_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBZ0280357.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(12642..15155)
                     /locus_tag="MJF80_001690"
     CDS             complement(12642..15155)
                     /locus_tag="MJF80_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBZ0280358.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGDINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(15378..16199)
                     /locus_tag="MJF80_001691"
     CDS             complement(15378..16199)
                     /locus_tag="MJF80_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBZ0280359.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(16717..17193)
                     /locus_tag="MJF80_001692"
     CDS             complement(16717..17193)
                     /locus_tag="MJF80_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBZ0280360.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(17227..17805)
                     /locus_tag="MJF80_001693"
     CDS             complement(17227..17805)
                     /locus_tag="MJF80_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBZ0280361.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(17909..20509)
                     /locus_tag="MJF80_001694"
     CDS             complement(17909..20509)
                     /locus_tag="MJF80_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBZ0280362.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLDTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTAVTNKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
     gene            complement(20962..21645)
                     /locus_tag="MJF80_001695"
     CDS             complement(20962..21645)
                     /locus_tag="MJF80_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBZ0280363.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(21865..22455)
                     /gene="zapA"
                     /locus_tag="MJF80_001696"
     CDS             complement(21865..22455)
                     /gene="zapA"
                     /locus_tag="MJF80_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBZ0280364.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(22480..24873)
                     /locus_tag="MJF80_001697"
     CDS             complement(22480..24873)
                     /locus_tag="MJF80_001697"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBZ0280365.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(24890..25909)
                     /gene="pheS"
                     /locus_tag="MJF80_001698"
     CDS             complement(24890..25909)
                     /gene="pheS"
                     /locus_tag="MJF80_001698"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBZ0280366.1"
                     /translation="MQEKLLALREAALSEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(25959..26193)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(26537..27334)
                     /locus_tag="MJF80_001699"
     CDS             complement(26537..27334)
                     /locus_tag="MJF80_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBZ0280367.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
     gene            complement(27523..28194)
                     /locus_tag="MJF80_001700"
     CDS             complement(27523..28194)
                     /locus_tag="MJF80_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBZ0280368.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            complement(28505..29881)
                     /locus_tag="MJF80_001701"
     CDS             complement(28505..29881)
                     /locus_tag="MJF80_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBZ0280369.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
BASE COUNT         9529 a         5483 c         3317 g        11907 t
ORIGIN      
        1 atatctatag taaatactta actatactta aaaagcatat aaattgatat aattatataa
       61 tagataagtt aatattgagg agggataatt atggaaataa gagtattaag atatttttta
      121 gcaattgcaa gagaagaaag tatatctggt gcggcaacct ttttacatat aacacaacca
      181 acactttcta gacaaattat ggaacttgaa gaagagttgg gagttaaatt attggttcga
      241 ggaaatagaa aaatcacctt aacaaatgaa ggtaggcttt tacataaaag agcagaagaa
      301 attatatcat tagttgataa ggttgaatta gaatttgaca atcccaatga gattattagt
      361 ggtgatatat atataggaag tggtgaaact gaagctatgc gacttatagc tagaactgcc
      421 catcattttc atcaaaaata ccccaatgta agatatcacc tttttagtgg gaatgctgat
      481 gatgtgactg aaaaattaga caagggactg cttgattttg gtattttaat tgaacctgca
      541 aatatacaga agtatgatta tgcacaaatt cctgctactg atgtatgggg tttactaatg
      601 cgtaaagata gtgcccttgc atcacaaagc acaataaatc caaaagattt atgcaatata
      661 cctcttatta tttcaagaca gacacttgtt gataaagcaa tttctaaatg gcttcaagac
      721 agtaatgaaa aactaaacat tattgcaaca tacaatttag tttataacgc ttctcttttg
      781 gtagaagaag gtctgggcta cgctctgtgt ttagatagac tcgtcaatac tacagaaaac
      841 agtaatcttt gttttagacc tctatctcct cctttagaag ctaaattaaa tattatatgg
      901 aaaaaacatc aaatattttc aaaggcagca gaaaaatttt tacttcaaat acaaaaagat
      961 ttttcttcta tcatgttgga tacatctaag ttggatacac ctaaagaaaa ataaatcata
     1021 ataaaaggtg agagtgtctc aaaatgaact ttgcactgta taaggcactc ttttttatac
     1081 aaagtaaaat atatgttcat catttcagca ctaaaaaaga ggcttatctc tattttgaga
     1141 tagccccact taaccaataa aattaaatac aacttaacaa tatccttact tatttattct
     1201 aagttctctt agacaagcaa tagtaatttc atcttgtgga tttaattcat aagctctttc
     1261 tatatagtaa gttgcatcat caatatcacc attatttaaa taagccattc ctaaattgca
     1321 aagtatttct gggtcttcct ttatcttagc cgcttgttca aaatactcta ttgctttatc
     1381 taaattattc ttataagcct cacaaagccc caattccact atagtatcca cttgattagg
     1441 ctttattatc aatatcttct caagatacat cttagcctgc tctatttcgc ccataccctt
     1501 atatccaagt gctatcataa acagcaagtt ccaccaatcc atatgttctt cttctaatgg
     1561 aagtaacttc tctaatcctt ccttaaactt accttggaat acaagtgtat atccttcttc
     1621 atattgaact ttaaagtcca tcttgcccag attctcttgt acctcagcta ctaaatcagc
     1681 atctaaccct agccttaacg cttcttccca agtcagctta gtctttaaat attgaccttg
     1741 attataataa tggtagccta agtgataata acctaatgca aaattctcat caacatcaat
     1801 taccttttca agcttagcca tggcttcaag taagaatgca ttcattgcct tatcatccat
     1861 atctttttga tactgtttag ctatctcttg gcaaacaatc gcatagttat acattgcatc
     1921 taaatcatct ggatacattg ttataaatgc ttttatatat attaaagaat ctgtatgttc
     1981 accaatctca aagtattttc tcgacatata tcccatatac gatttcaaat ccaaatcaat
     2041 atcttttgat aaagcattta aaaacttctc atactctgca ttgtttttaa acttactatc
     2101 tattccaatt atatatatca ttgcatctgc tatagacatt gaatttaact tatcttgagc
     2161 agtcttttct tttatccctt ttactaatac ctcatttttt ataggtacat ctaatccacc
     2221 ttttggcact tcatagcttt ttaatttaaa ctctccacca tgttttatag ttataaaagc
     2281 taattcttct gcttttttta gcaaatattt ttctatctta aaatccattt tatcctccta
     2341 aatacttaaa tctctacttt atgagtttta gatttctcaa atattttttt atcatcatac
     2401 tcaaataaga ctacattttc tacttctctt atttctgtat ttatatcatt ttttaaatcc
     2461 acaacattaa atctaaatgg ctcaaaatga acatatttta ttatatgttt agctggttta
     2521 tcaacaaaac taggtaaata tgttgataag ttatcattat cttgtaaaaa tacatggcat
     2581 ctattcttaa aatcatccac atctagctta ttaaaaaact gtggtatatt tgatgtttta
     2641 cccagtgata tatagtcata ttttaatata tcgttgaata catcattatc tatgttgatt
     2701 ttttcattat agaaatccat caatatttta tataactgat tctttccttg tgccaaatca
     2761 aagtagccat tttcatcaaa atacttggca aaaacctcaa agaatttaaa agggcttttt
     2821 ttataaaatc tgcctataat atatctcata gataacacaa agtttttaga attgtagtat
     2881 ctctccagta tatcttctat atcctttagc ttcaaagttt cttcatagct tatataatta
     2941 ttgtataaaa actcatatgg agcataatcc ttatatttaa aaccatgctc atctgcaact
     3001 tttctcatac ctgtcccttt aatcatcttt aaaaagccta attgcaaatg ctctatacct
     3061 agattaaata catcattaaa agaattttca aagctcttat aatcttcata aggcagtcct
     3121 gcaattaagt ctaagtgctg atgtatattt ctataagatg ctacagtttg tacaacatga
     3181 gacaactttt taaaatcatc ccttctacct actgcatcaa gtgttttttc attcgttgac
     3241 tgaacaccta tttcaaattg aaaaagacct tctttacaat cagccaaaaa ctcaagcatc
     3301 ttatcatcta tcaaatacgc tgtaacttca aaatggtaag ttgtatatcc attatcattt
     3361 ttcataagga aatccatgat ttccatagcc acttttctat ttgcattgaa tgtcctatct
     3421 ataaacttta tctgtgatac tcttgcatct attaaagctt ttaaatcact ctttatcctg
     3481 tctaaactaa aataccttag acctggaata gtagatgata aacagtattg gcagttaaaa
     3541 gggcaacctc ttgatgattc ataatacact attctatttt catattcttt tggattcaag
     3601 ttttcataag gactaggaat ctcatccaaa tcttgaagta attccatagg cttattcact
     3661 attatctcat tgccttgtct atatacaagt ccatttacat catttatttc catctttcct
     3721 tgcaagtgaa gtactaagtc tctaaaaatt aattctccct ctccataaag tatataatcc
     3781 acaaaatcat actttttcat tgcctcataa ctatcataac tcacttccgg tccgcccaaa
     3841 gctattttag tattaggtct aatttttttt agattatcac aaagtttaac tatatctccc
     3901 acattccaga tatatgttga gaataatatt atatcatatt catttttata tatatccttt
     3961 aaaatatagt ccaaatcgtt atttatcgtg tattctttca tattaacttc tatcaaatct
     4021 cttacaaatt cttttagata tcttatagcc aaatttgtat gaataaactt ggaatttaat
     4081 gttgttaata aaattttcaa atatttctca cctcttttta agattatact gtctttttgt
     4141 accccttata ggttaaaata gtatatgtaa agtctaaata atatctatat aattaattta
     4201 ttaatttcaa gtaatgtgag gaaaggaagt tttttatgga attaattgaa aaagtatcta
     4261 tcaactcttt atcaaaaaga gatttactac ttattataaa gtctcttgaa tacactaatg
     4321 aaaatactca aattagtgat ttcattgacc ttaggaataa tatagtaaag gaattatgtt
     4381 ttttaactga cactaaggaa aaagattttt tagattatct agaaaaaaat agcacactct
     4441 aataacctaa acctttttaa actttaatat ataaatacct gatttagaat ttatttttat
     4501 gcttaatata ttatcattaa aggttttcga cataactgtt atttgattaa atatagggtc
     4561 tattatagta ttaccttctt caagtcttat aagacttctt gggtctatgc ttttattata
     4621 tgccttaact ccaattttat attttctcaa tttaacaaca tattcggaca tagaaaaaat
     4681 aaaatctata ttatcttcat ttaatttact atcttcaccc aaaatggata atatagattt
     4741 ttttatgcta ttaattcctt cattttctac agttctatct tccatatata catcatatag
     4801 aacatttaaa acatttaata aaatactttc ttcatcataa tcttcagatt tcaaatcaat
     4861 gtacttatca attaattgta ttgctttaat ccttgccatc tgaaacttta caacatctga
     4921 tttgtctaat tctatagaaa atcctattat cttgtcttta atactctgta gtaattcgac
     4981 atactctatt ttactattat ttataagatt gtgcataatg gaattataca ccacagaacc
     5041 taataaataa tcaaataaaa gttcttcttt tttaaaataa ataacatact tttttattag
     5101 tttaataacc tcatcttcac ttttttgaat attagtattt gataaaatta gtggtaataa
     5161 aatatttaaa aaattgatat ttcctatttt ttctccataa aatctatagt aaataagttc
     5221 atctctcagt ctattttcgt ctatatacac acatctttgc gttttatcat ctattaagga
     5281 ttttgtattt attatagtta aatctttaag agccctactt gcactactaa aagaagatgt
     5341 gtagactttt tttagtaatt cgtcattcat aaatatatcc tttctgaaaa attgaatgta
     5401 aataaattgt tattaaaaaa aaagagacag cccgtttaag aagctgtcat atataggggg
     5461 atagtagtgt acaaaaatca ttgtaatcta ctatctatat attattatac gatactactt
     5521 ttttatatgt aaagcatcag ttttcgacat ccctatattt tacagttata caaattgcgt
     5581 actaagttca attttattct ttttatttca acattttatc atgctacatt ctttcaggag
     5641 cgtgcattcc caacaatttc agagaatttt ctaaagtttg ttttgttgct aaaactaatg
     5701 caagtcttgc ttttcttatt tccaaatttt ctactaaaat tggattgtca tgataaaatt
     5761 tattaaacgc ttgagctaaa tctagcataa atctagtcac tatatgtggc tcatttttct
     5821 ttagagcaag taaaatattc ttattgaatg attctataac tctaagaact tctgcactat
     5881 cttcatcagc taatagacta taatctatat cagaagtcac ttctacttct gcttttctta
     5941 aaactgcaca gcatctagca tgtgtatact gaacatatgg tccagtctca ccatcaaaac
     6001 taagcgttct ttcccaagag aaggtatagt ctttaattct actattagat aactcttgga
     6061 atactactgc cccaacacct acttgcttag atatttcatc cacattttta gcatttgggt
     6121 ttttagctag tataatttcc ttagttttat ctatagcttg atttaaagca tcttctaaaa
     6181 atactactct tccttttcta gtcgacatag taccttcttc aagtgcaacc ataccaaatc
     6241 caacatgaat taaatctttt gcccaatcat atcccataag ctcaattacc ttaaaccatt
     6301 gttggaaatg taagttttgt tgagaaccaa ctacatatat acacttgtca aaatcataag
     6361 tttcttttct atatattgca gcagctaaat ctcttgtcat atataacgtt gagccatcat
     6421 tcttagtaat aagtgctggt ggcattttat actcttctaa atcaacaatt cttgctcctt
     6481 tagactcttt taaaagattt ttctcttcta atagttcgat aactctgttc attttatcag
     6541 aataaaaact ctctcctgct aaagaatcaa attctatatc taaaagttta tacactctgt
     6601 taaactcttt taaactctca tttctaaacc attgccataa ttcttgtgct tcttcgtcac
     6661 cattttctaa tttattaaac catgctcttg cttcatcttc catttctggg tgttgttcag
     6721 cttcatcatg gaatcttacg tataatttta gtaattctgg aattggatta gactctacta
     6781 cttctttttc tccccacttt ttaaaggcca ctattaactt accaaactga gttccatagt
     6841 cacctaaatg atttattctt atagtcttgt atccttgcga atcatatatc ttatataaag
     6901 cattacctat aactgtagtt cttatgtgcc ctatatggaa aggttttgct atatttggag
     6961 aagaatattc aactattaca gttttatctt ttccaaaatc actatgtccg taattttctt
     7021 tttcatctaa aactttttta attacagttt ctgctaattg agatttattt acaaagaaat
     7081 ttacatatcc gcctaattgt attacttttg ttatctctcc acttggttct atactttccg
     7141 ccaattcact tgctatcatg tttggagctt ttctaaacac tttggctaat ttaaagcatg
     7201 gaaaagcata gtcccccata tccttatttg gtggaacttc tataagtgcc tctatctctt
     7261 ctttcgataa atcttctatt ttttctttaa ggcaatttga tattgccacc ttaaaatctt
     7321 gcattctgtt gctcctctct acattgttcc tggtttatat tgtgcttatc tctttatgtt
     7381 aaatcaaatt tcgtgttttt tcaagtattt cattacagtg taaaacactt tagtaattat
     7441 gttttctgta tatattaaaa atactataat tgcagttttt aatcacacta ttattgattt
     7501 tttataagac ttttttctat tttattgttt cttgtactat ccagttgact ttatatactt
     7561 tttttaatct tttatctatt ttcatacgtc aatgctaatt ttatatactt ttcttttaat
     7621 cttttattaa tttttataca tcaatgttaa ctttatataa gtttttttaa tcttttatct
     7681 gttttcatac atcaatgtca gctttatata ctttttttac ttttttatct atttttatac
     7741 atcaatatta gctttatata ctttttttaa tcttttatct attttctata aatctttttc
     7801 actcttaact tttattcctt cttcttctaa tagccttgct gttatacctt tccctggtat
     7861 ctttttacct gaaaaactac catcatatat tagattacat ccacaagagg ggctaccctc
     7921 ttttagtatt gcttcgctac atacatataa atttgctatt tttagagttt cattagcccc
     7981 tcttacaaag ttttctgtta catccttatt atctttagta attacttttc catcaactat
     8041 ctcagcaggt ggtcttggtg tagatagacc acctaactgc tctgggcata caagtgtaaa
     8101 ttctttgcct tttaaatatt ccttgacttt ttcattatca ttattatcgc cactatattt
     8161 acaatttatt cctattaaac atgcagatac caatatcata aaaatgtgct cctatacaaa
     8221 ttcttaatat attctcatac aaattactaa tgtattctta tacaaattac taatgtattc
     8281 ttatacaaat tattttaatt taacaattgt tactcctgct ccaccttctc catattgacc
     8341 acctctttgt gattttacat gtctatttct tcttaaaatg tcttgtagcc ctgcttttaa
     8401 tactcctgta cctattccat gtattactgt tacagactct aaacctgcca catatgcatc
     8461 atcaagatac ttttctactt ccattatagc ttcttctagg tttaaccctc taaggtcaac
     8521 ttcatttttt acactaccag attttgacct tataatgttt ctcgttgatt tagttacatt
     8581 tgttgaaaca tctttgcgtg ttttttgtaa agacttaaaa ggtagagtca tcttcattat
     8641 accaatttgc actactgctt cttttctctt tttatctact gaaactacac taccattttg
     8701 atttaaagtt attaccttaa cttcttcacc tggctttaag tcttttatct ccttatttga
     8761 tacctttgga actatcatac tttttactgt tggctgaaga cttcccatag agctggtcaa
     8821 ttcttttcta agttcttcta tcttacgatt cttttctttt gaagctcttt cttgttcaag
     8881 acttctaagt tcttttatta taatatctac ttcttcttta gcttgtctta taattgaaaa
     8941 tgcttctgat ttagcttttt caatcatctt atctctttga ctgaccaatt tttcaagctt
     9001 ttcgtcatac tctactttca gtttttcaat ctcttctttt aatctttcag cttcttctct
     9061 atcttctact gctttgattc tattcttttc aacattctgt aacacatctt ctaaagctat
     9121 gttttctgta tttatatatt ctttagcacg tgaaatcaca taatcactta gcccaagttt
     9181 tctagaaatc tcaaacgcat ttgattttcc gggaacacct ataagtaact tatatgttgg
     9241 gcttaaagtt tctatatcaa attcaactgc tgcattttct acacctggtt tagtaagagc
     9301 ataattttta agctcactat agtgagtggt agctatacat ttagctccta cagaatttat
     9361 atcttctaat actgctattg caagggcagc tccttcaaca gggtctgttc ccgctcctaa
     9421 ctcgtcaaat ataactaaag agtctgctgt tacgttttgt aatatagata ctatattcgt
     9481 catatgagat gaaaatgttg atagactctg ttcaatactc tgctcatctc ctatatctgc
     9541 aaatacatta tcatatacac acatactact tccataatca gcaggtatat gaagtccact
     9601 ttgtgtcata agtgcaaata gacctacagt ttttattgta acagtcttac cacctgtgtt
     9661 tggacctgtt atcaccaatg tatggaagtc tcttcctaaa taaattgtat ttgctactac
     9721 cttcttttta tccagcagtg gatgtcttcc attttttatg tttaaatatt tatcctcatt
     9781 taaagttggt tctattcctc tcatctgtat agaaagtttt ccctttgaaa atgcaaagtc
     9841 caatcttcca agtatctctt gattagaaat taagtcttca ctaacttctc caaccatagc
     9901 agaaagctct gataaaattc tttctatttc ttcttgctca cctagtttta actgtctcaa
     9961 ttcattgttc atctctacta tagtcattgg ctctataaaa agtgtcgcac cagaagatga
    10021 ctggtcatgc actataccag ctacttgcga cctatattca gattttacag gtactacaaa
    10081 tctatcacct cttagagaaa ttattgcatc ttgtaagtac ttttgataag tagttgaact
    10141 tattattgaa tttaattttg acctaatgga ttgatttttt tgggctattc ttcttctgat
    10201 atctctaagt gtacttgaag cattgtcaga tatttctact tcactaatta tagcattgta
    10261 tatctggtct tctatgtcct tgtatacata tagagaattt gatagagatt gtattattgg
    10321 ataattaaaa tcttcttcat cactactaga taagctattt cttaatcttc tagccactct
    10381 taaggtatct gctatcataa tcagacttcc tgggtcaaga gaagcaccta tataagctct
    10441 ctttacctta tcttctatat catgaatacc ttccaaccct actgaacctc tctttataat
    10501 aattgattga gcttcactag tttcttctag catagatttc acttctacaa aatcagtatt
    10561 tggtactaaa ttttctatat attttagacc taaagaagat gaggctttct tctttaacaa
    10621 atctattatt ttattgtatt ctagaactct taacgatttc tcattcattt tctatattca
    10681 ccatcctcga tattttaact tttttattta attttaaaaa tgtatgtttt caagcatttc
    10741 caataaaaac tgcaagtttt atttcttttt catttctaaa attattatat tttttctctt
    10801 ttagatatat tttacacata ttttgtatta tactatgtat gtagctatct atatattata
    10861 cataaacatg ctttatttgt aatataatct gttaaaaaat cttctttaat atttttaaag
    10921 gagtttattt tagcttataa aaaaaaatag ctacttatat agataaatta aggaggaaca
    10981 tgagtcatgg gaaacgtatt agaaggatta aaaccagaaa gtgtttttaa aaactttgag
    11041 aaaatatctc aaattcctag aggttctgga aatgaaaaag gcataagtga ttttttactt
    11101 agctttggta aaaatttagg tcttgaaact attcaggatg aaagcttaaa cataataata
    11161 agaaagcctg ctacaaaagg atatgaaaat tgtccaggtg tagttcttca aggacatatg
    11221 gatatggttt gcgaaaaaga aaagaatgtt gaacatgatt ttcttaaaga ccctataaaa
    11281 ttaagaattg atggtgacat gatatatgct actggaacta ctctaggagc tgacaatggt
    11341 atagctgttg ctatgggtat ggctatatta gaagataata ctttagagca tcctgcacta
    11401 gaagttttag ttactgttaa tgaagaagat ggtatgaatg gtgcagatgc actagaccct
    11461 agcttaatta aaggtcaata tatcctaaat atggactccg aggaggaagg atacttatta
    11521 gttagctgtg ctggtggtaa aacttgtgtt gttagtttac ctgttgaata caaggaagtt
    11581 aaaggtgaca aacaaggatt attagttgag gttactggac tattaggtgg acactctggt
    11641 atggaaattg ttttacaaag agcaaatgct aacaaagcaa tagctagagt attaagtgta
    11701 ttgaatgttg attatgaatt agcttctgtt gatggtggta ctaagcacaa tgctatacct
    11761 cgtgaagcta aatgtgtaat agctgttaat aaagctgatg ttgaatctgc taaaaaacaa
    11821 ataaatgaca ttttaactgc atttaaacat gagtttacta cttctgaccc tggcatgact
    11881 tactcagtag ctgaaacttc tgtagacaaa gttcttacta aagattgtaa agaaaaagtt
    11941 gttcaaatgt cttgcttaac tcctcatgga gttcaatcag taagtcttga tatagaagga
    12001 ttagttgaaa gttctactaa cttcgctata atcgaaacta aagaatctac tattgaattt
    12061 ttaacttctg ttagaagttc tgttatgtct attagagatg aaatcgctga tagaataaga
    12121 cttttggctc aagctttagg tgctaattac gaccttatag ctcaataccc agcttgggaa
    12181 tttaaaaaag gctcaaaatt agaaaaaata tgcagtgaaa cttatgagaa attaactggt
    12241 aaagttccta ctgttatggc tctacatgct ggtcttgagt gtggattatt acttgataaa
    12301 cttcctcatg ctgaagctat ctctatagga ccagatatgt ttgatgttca tactccaaat
    12361 gaacatgtta gtatcccttc tgtagctaat gtttgggatt atgttattga aatattaaaa
    12421 tctatgaacc aatattaaat ttaagttttg attctaatta gattttaatt ttaagtaatc
    12481 tatataaaat taactctgta aattaatact actgttagta taatatttac tttcaatatt
    12541 aacttataga aataaagcct aatgatagat aataacatgt atcattaggc tttattgtaa
    12601 tttaaatatt attaagcttt attctgtttt aaaattaatt cttatactat tttaaatcta
    12661 tttcttcctt gtctcttaga atcataaaga gcattatctg ctatttgata cagttcatca
    12721 tactcttgac caggctttgc tataactacc cccatactaa tagataaact ttctccattt
    12781 ggcaacttaa tgagatgaat atgattgtat acatcattac atttattaat aagttcttcc
    12841 ttatttatta tatcattgat ataaacacta aattcatctc cacccaaacg acctactata
    12901 tcattttttg caaaactttc atcaagtacc tttgcaacat caataagtac tttatctcct
    12961 aacaagtgac cataagtatc attaatcgac ttaaaataat caatatctat aatcaataaa
    13021 gcaccatttt tttcagttga taaattcaac aaattttgtg cagttaattt tctaatatat
    13081 gctgcattat aaatattggt taaactatcc ctttgagcct tatctaacaa tctattcttt
    13141 tcctcttttt cagaatcaat attacatatt ttcccgatta catacattgg tatttttcta
    13201 tcattataaa ttatttttga agtaactcta atccaatgaa aagtatcatc aagcataata
    13261 gaatacattt cagttgttcc attttcttgt gattttatta catcaaaaaa ctttttatta
    13321 tattctttcc ttattttatc tctttcattt tctatattgc cattttcact ttccttatca
    13381 tttttatcca aaccataata agtctcaata cccttttctg gcatagaaat tatcaattta
    13441 tcttgcttat aatcatactc aaaaaagtat tcacttgtca tttcatacaa ctgagtatgt
    13501 tttttgagtt ctaaagatat cttctcgttc atacttgtac gagtatgcaa accaaatgcc
    13561 ataaatccaa taatacaaat taataaactg gctacaataa aaatagtctc aattggattc
    13621 tgttgcataa tataaagcaa cgagaagtct tgtctatatg tagtattttg gtagacaatt
    13681 gattgcattt tttctgatga aatacttata atagctttat taaatatatt caataattct
    13741 tgtttatctt ttttaattat accaaaacaa atactctttg ctttataagt ttgaggaact
    13801 agacgcacat ttttatactt aggttgattc atatagtact gcatagaata accttcgcca
    13861 taagtgtaat ctgcatagcc tttattaaca gcttctatac actcttctaa attatcatac
    13921 caaactatat tctcttcaaa ctgaccataa taattaatac ctttggctag agctacacgt
    13981 tttccatcta aactattctc atttaattta ttattaatca tcatagtata ttgagtagaa
    14041 agaaaaggcc gagacataac tatattatgt tcacgagagt attcataatc ataaatcatt
    14101 cctgctacaa tatcaatttc acgcttttta gtcatgtttt ctaaatcttc ttgagatttt
    14161 acaggcacaa gttcaaattc taagcctgtt ttatcacata tatgatttaa caaatcaatt
    14221 gatatacctc ttaattcacc tgtatcttta tctatatttt gaaatggtgg tttgtttaaa
    14281 agtactccta cttttaattt ttctgtctct ttaatgtaat cagtttcttc atcagataaa
    14341 aacatttgat tgttctctgg tgtaaagtat ttttcatata aagtagttga aaaatatggg
    14401 tctttctgtt caatattatt aattgctgag ttaagctttt gagttaaatt tgtactacct
    14461 ttaggagtaa tgaaataaaa tggttttgat gaaaattttg ctacagtacg tacaccttct
    14521 agtgggttca tactcacatt aagaagtaca tctgctttac cactctttag tgcttctagt
    14581 tgctcatcat ttgttttaca atataccaat tcaggtgaca taagattcat cttacaatac
    14641 tcatctaact ctttaagcct agtatcagaa ttttctatta cagcaatacg gatttttttc
    14701 acatcttgtg aactcattat gttatcaata tctgtatttt catataatgc ttgtagtact
    14761 gtatttgcta ctccataatt attccctgca taattatata ttttttgcat tggctcattg
    14821 tataacatac ctcccattat atcaatttca ccattttcta acatcttcat taattttgta
    14881 atagattcat ttatatctcc aggaacttga acaaattcat aattccatcc agtatactgt
    14941 gcaatttcta gtaaatattc atatgtatac ccaaaatagt taccttgctc atctatttca
    15001 gttaaacctt tttgaatagg ataagcaaca cgaatggtac gattatcatt acctatagcg
    15061 ttgataccaa aagaaaatat gcaagaagtg aatatagatg taattacaat aattaataaa
    15121 agtaattttt ttaaattcac tacacaatcc tccatatagt taagatttac agtataaatt
    15181 actgcataaa cttgtagaca tgtaaaaata gattagtata taaaaggata tataaatcta
    15241 cttagaaatt taaatatcct taatatatac aatatattta aattataact catttatatg
    15301 tctattacaa caaatttacc ctgctaaata cctactctat atcccataat tttacaagtt
    15361 gtagtgaagt ttaagttcta tctcacctca aatttagcag tgtttctatt tacagttctt
    15421 ttatatttca catctggata acccaatata aatgaagtga taacagcttc gttctcgctc
    15481 attcctaaca gttcttttat ctttggctct acatttgatg ccataacaaa aaatccatta
    15541 taacaaattc ctaatccttg agaataggcc ataagttcca tattagatgc agccagtcct
    15601 ccatctacat aagcacttcc acctagagac atatctccta caactaccat aagtagtggg
    15661 gcatcataaa ataaagaatc atttccgttc tctttatatc ttttgtacat tttcttaaat
    15721 gtatttttat agatagaacg tacagggtca ttgtcatcag tatctaatgc caaattataa
    15781 agaccttgta ttgccagttc tttcacttct tttagctttt cttttactaa tatgtatctt
    15841 ataggctgtc tattaccacc tgttggtgtg tatctaccag cttccactat gttttttatt
    15901 ttttcttctt ctacctctat atctttatat tgtcttatac ttcttctaaa tttaataaaa
    15961 ttcaataact tttcgggttc taattcaaaa ctttcacgat tgtattctat tacttcattt
    16021 ttatcaaatc cttgcatttc tattgcatct gttgggcaaa ttgccataca gtgaccacag
    16081 ttaatacaga gaatattttt aacctttgct ttatcatcct taacttttat tgcatttaca
    16141 agacagtctg attcacacat accacagcct acacattttt ctaaatttat gtctatcatt
    16201 ccaaattatt cctcctttta aaaaatttac tttagtataa aacaaatagt tgtctaagtc
    16261 aatttttatt tttttaacaa tatactagcc ttaactacac tttaatggag aaaaatatac
    16321 ataaattgga acttaaatta gaacttattc aaagtaaaag ataaaagaag tagatgtcta
    16381 aaagtgaact ttttagttaa ttagatattc tttattgtat gaaatcaagt ggatatttat
    16441 catttcaaca ataaaaaagg cttacctcta ttttggatat gtccctgtca agtagacaga
    16501 ctaaaaaaag gctatcttta tgcactgaga tagccccttt tcccaactac tattgtttat
    16561 cttaatatta gtcaataaat ttaatttgct ataacctgta agttacattt taatttgatg
    16621 taactttaaa ttacattttt aagtaatatc tttaaattat attcttaaaa tatatcatta
    16681 aactacattt taattaatct aaaaactata tcttaattaa tctaagaaac ttacattata
    16741 atcttttagt ttcccattat catatacaat ctttgcacta aagatatttc cagtcttagc
    16801 ccaatttaag aacagcctgt caccttccca catatttaaa tcgaatattt tatcattatc
    16861 aacccaaatt aaatttcctt ctttacaatc atttaaatct ttttcagata attcaccttc
    16921 aaactcatct gagaaataca aaaacatata ccaatcttct tcatctttaa attttggaaa
    16981 agttatcaac cctttatatg acaatgattt aagagtaaga ccagtctctt cttttacttc
    17041 tctaattaca cattcttctg gggtctctcc ttgttcaaac tttcctccaa ctcctacata
    17101 cttaccttca tgtatatcat ctttcttttt atttctgtac aacatcaaag tcttgtcatc
    17161 tttttctata taacaaatag ttgtaagttt catgtatctc gccttgcttt ctctttatat
    17221 tctcaactaa ttattttcag cactttcaag cacatcactt atattcttca ttgcattaag
    17281 agaaagctct gcaaattcat caaatggaat ccctatatct tctatacttc tcattgcatc
    17341 tctatttaca tttgaagcaa atctcgtttc tttcattctt ttaataacag acttagtctt
    17401 tacactggta agttttttat ctggataaac taaagctata gcagttataa acccagttat
    17461 agggtctgct gcatatattg ctttttccat taggctagtt cttgtaactc ccattccatc
    17521 atgatgacca agtattgctc tatacatttc ttcgtcaccg aagttttctt ttttaagcag
    17581 ttctactgtt gttgtagcat gtccttcaca tatttctcct ggctttcttc caactatatc
    17641 tgaatccaaa tcatgtaaaa gacctgccac tgcccactct tcttcttttt ctggctctag
    17701 tcttttggct aattctctca taacagcttc aacagcataa caatgttttc tcattctatc
    17761 agtttctaaa tacttaaata aaatttctaa agcttcttct ctcattttca ccaatccccc
    17821 tgttttttca catatatgat atttttatat attaatacac ataaattcaa gtttttatat
    17881 atattcatgt atatttttca tgttatatct attctacacc tttatagtag tgtcctgcaa
    17941 ctgttccaat ttcatctaat ttcttataca agtctgtagc cttttttcca agtttataat
    18001 tattactcaa tacctcaatg tgagcttcaa taaccttttt aaccatctca ctatcttcaa
    18061 ctgtaaaatc tatcctaaat acatctattc caatttcctc taactcatat agattgtcca
    18121 acatacaagt aaccttcgaa ttgtaaattg tacttctaca gaatatatct tgtgttatcc
    18181 tataacttgc atccgtaaag tctcttaaag cataactact ttccttacat ttagcaactc
    18241 tcttgtcctt tttacaatct ctaacaacta ctcccatagg acagtattct gtaaccatca
    18301 taggagcata tccatatatt actgcttcaa tttctacatc tgtatatttt aaagtttgtt
    18361 taatttcatt taagtttagt tcttgtgaca aacaaactgt acttgctccc tcttctttga
    18421 agtggtttat actttcacta ttgaatgcat ttaagtagca atctatattt aagtcttttt
    18481 tcttaaagta ttccatagag ccccagttac caatttgaac cttattaatt ccttttatat
    18541 tatccaatat ctcaaattgc ttgtactctt tatttctaac aatccttggt gctgaataga
    18601 ttaatttttt gttagtgact gctgtattat agtggttctt attcaaagta tctattttac
    18661 ttgtatttga caaagtatct atgttatttg tatttgaacc cattagttct ttaggagtat
    18721 ctaaagacga agaagtatct aaatccatag aaatacttga atctatagaa atatttgaat
    18781 ctatagaaat acttgagacc atttgtaaag cttttgataa tgtatttata tcttcataat
    18841 agattaaatc tacattataa tttaatactg cttctaattg ctccaagttc ttaactttaa
    18901 ctctaatctt aggaactgta tcaagttttt tctgtttatt tactctattt ggctggtatt
    18961 ttaatctatt cttcttaaat tttctatctt ttatctttat tctttcttca tctaaaagct
    19021 caatacaatc ccttctcatt tgatttaaca cacttatagg catacttaca ccttcatcca
    19081 ggtcaatttc tatctctttt aacttatatg gtgtatttcc aagcttttga atttgtgttt
    19141 ctattttttc tttacttaaa gccaccttta tagctttttc aactattttg tctccttcta
    19201 ccaccgattt atttccacaa aaatcactta taaaaagctc tggcttttta cctagtttaa
    19261 tactcacttt tgcacgtata ttagttttta tgaactcttt atcttgctga aatgtagctt
    19321 gtactctatc catcaattcg ctgtcagaag tcttatatat aagctgattt ctctttgctt
    19381 ctccaacaaa atctaattct atcaaatccc ctttatgtgc tattgtagtt atctctccat
    19441 cctttattat tcttccaata gtacctccac ctaagttaat tccatcacct ttcttaagcg
    19501 tattttctag cattactcta agtcttttgt tctttctatt ataatctaaa acttttccaa
    19561 tatatagccc ttgattatta ggtctattag agttcattat atccttacca acttcaccta
    19621 atatatatcc ttttgtaaac tttctattaa atatggtata caaattattt attatttcct
    19681 cagaaatact tatttttttc ttatctacaa attcattaat cgcttctcta tatcctgaaa
    19741 ccacagtagc tacatactct ggctttttca ttctaccttc aatttttaaa gataatacac
    19801 ctgcttcaat tattttatcc aaatcttcta tagtacttaa atctctagga ctcaacaaat
    19861 aatctccatt actttttact acttctcctg tatttatatc tatcaactca tacttttgtc
    19921 tacatggctg tgcacaccta cctctatttc cagacctatt gcctatcata ctactcataa
    19981 ggcattgacc cgagtaacat acacataaag ccccatgtac aaaaacttct atatctacag
    20041 ttgtattttc acaaatccat tttatctcat caacatttaa ttctcttgct aaaacaactc
    20101 tatcaaaccc tacactctga aggtatttta catcttctaa ggaatgtgct accatttgag
    20161 tgcttgcatg tagttcaaag tcaggtaata gtttttttat aagtcttgcc attcctatat
    20221 cctgtactat taaagcatca atatctatat cgtataaaaa ttttacatac tctacaaaat
    20281 cttctatttc atactgtttt atcaatgtat ttacagtaac aaagacttgc actcccctta
    20341 tatgagagta tttaacagcc tcaatcagct catttctatc aaagttattt gcagatgctc
    20401 tagcactaaa gtctttacca cctaaataca cagcatttgc tccgttttga actgctgctt
    20461 ttaatgaatc aaaagagcct actggtgcaa gtaattctat ttttttcata tttttctcct
    20521 ctatttatac ttgttaggtt aatataatta tatctataac ttaaatattt gtattaaatt
    20581 tatatatttc ttactatatt ttaaattctt caatcaattt atatattagt tatttatatt
    20641 tggttcattt gagttaacat atacattatt tactatattt agtatatttc tattaactta
    20701 tatatttatt actgtatttt aaattcttca attaatttat atactgctta ctaaatttaa
    20761 aacattttaa ttaatttgta cactgtttac tatacttgaa atacttctat taatctatat
    20821 attgcctact atacttaaga cattttaatt aatttataca ctgcttacta tattttaaat
    20881 tctttaatta atctatacaa tatttgctat atataaagta tatcaaataa cagcttcatt
    20941 tttttgtaaa tttacagaaa gttaattttc attaaatgaa tcatctttat tgataaacat
    21001 aattccaaag accactgcca aaataaaaaa acatattagt attgattttt ccacgaaaat
    21061 ttttgtaaaa aaagttccta aaatagctcc agatataaag aatataataa tagcataata
    21121 ctgtaaactc ttttttatca taaaaatatt cttagtatgt atataatgaa ataaagtttc
    21181 tgtagcactt cttaaatttc cagtacacat tgtcgttgca aaactattcc catttacttt
    21241 tctaaaactc tctacttgca ttgaacaaat aaaagaaata gcaacattta caaacatatt
    21301 aaatcttcct ctaggaataa atgaaacaat aagtattgta atcatttcaa ttataatgat
    21361 aacctgacgc caatgaatct ttgtcttatt atttgtaaaa tactttctta tcttctcggc
    21421 tataaataca cctaacataa atgccaaaat aggaatcaaa taataaaatg attcctttac
    21481 attaccttct gctatattaa gcccaaacaa aactatattt ccagtttgtg cattagcaaa
    21541 gactttacct cttaccacat aagtataaat atccaaaaat cctcctgtta ttgccaacaa
    21601 agaacataaa aagaatgatt cagacatctg actcttgctt gtcatatata aacctctcgc
    21661 tttctaatga tagatacaaa atcaaaatca ttttagattc tatttttcat tgatataaaa
    21721 aaattattta tatttaaata ttgtctatat ttatacatca tttatattta tttatattta
    21781 aacattatct atatctatat agcatttata tttatacatt aaaagaagct gtctcaaaaa
    21841 acaagagaca gcaaatcttt tagtttattc cacatttttt gcatcattat ttaaccctgt
    21901 acattttaac tgtaaatcat aagctttgtt ttgaaactca gaagctaagt tttcagctat
    21961 agtagctctt tcatttgctt cttcaagttg agtacttaac tcctcaatct tgtttttata
    22021 agcatctaac tctgctctag aaccttctgt cgtattacta agttcgaata tttcttgctt
    22081 tttattttct ataatctcaa ttaactcttt catcttagtt tcagcttcac gattttctgc
    22141 ttctttttct gctatcgtaa gctttaaaga tttgatttct aattgaagct cttcattaga
    22201 tgctccaacc ttcttactca attcttcatt cgctttaatc aacttctcat tctcatctga
    22261 acattcaaat aaaaggtctg atatatttaa tgcagttaat atcgcagcaa ctgataagct
    22321 caatttcggg ttttgacgtg ttattttatc catttctttg tctacaaaat cagcaatact
    22381 aatcataaac ttctctgatt tgtctccaac cattggatat tcagcgccat ggattttaac
    22441 cattactttg ttcatcctaa cctcccccga cattagttgt taatttgatc ttaagttagc
    22501 atttaatttt tcgcttaatt cacttaatat cttatcatgc accttagcta catcttcatc
    22561 tgttagagtt ttatctttac ttctataagt tattgaatat gcaattgact tgtatccagc
    22621 ttctatttga gaacctttgt aaacatcaaa taacttgtag ctttctacta atccttgacc
    22681 attttccttt attatatctt ctatttgttt tacaaataca tcatctttaa caataagagc
    22741 tatatctctt gatgtagatg gatattttgg taatggcaca taattctttg ttctatcaga
    22801 attatcaaat acaaagtcaa tatctatttc agcgacataa actctttgac ctagattgta
    22861 gttttctata acatctggat gcaattcacc taatgtacct acatatttat tattatacac
    22921 tattttagca catctaccag ggtggaaggt agtattatta tcttgaggtt caatttcata
    22981 acctttaaat ccgacattta caagtatagt ttctatagtt ccttttagtg caaagaagtc
    23041 aacatcttta ccatacatac ctacacacat tctattttct tccttaggta attctgaatc
    23101 ttgtgggatg aatatatgtc cacactcaaa tgcagatact tcttctattt tatgagatac
    23161 attagttgat aaaacatcta acatatttgg tattaaagta gttctcatta cagaagtttc
    23221 ttctcctaat gggttaagta atttaacaaa atttctcttt gcattacctt ctggtactct
    23281 tatcttatct actcccttag ggcttacaaa tgaatatgtt aatatttcat ttaatcctac
    23341 agcagttgaa ttatctttta aagcatccat gaatttttgt tttgaagttt taattcctgc
    23401 tgtcgtattt ccttctaatt gtacagaagg tatattctca aatccataaa ttctagctat
    23461 ttcttcccat acatcagctt cttgttccat atctgttcta aagcttggta catctatttc
    23521 taatttatca ttagctacca aattacattt aaactctaat gattctaaaa ttcctacaaa
    23581 ctgctccatt ggtacatcta cacctaatag gtggttaatt ctttgaggat ttactaccaa
    23641 ttttttaggt tctggtttat ttggatatac atctacaaca ccttttaaaa cttttcctgc
    23701 tcctaacatt tcaacaagtt gtgcagctct atttgctgct atctctgcaa gattagggtc
    23761 taagtctttt tcatttcttg aagatgcttc tgacctaata ccaacttttt tagctgtcat
    23821 tcttatgttt tctggtttga aattggcact ttcaaaaagt acagtcttcg tattagaagt
    23881 tatttctgag ttagcaccac ccattacacc agctaaacca agtgattttt ctccatttgt
    23941 tataactagc atatctttgt ctaatgttct ctcaacatca tctaatgtta caagtttctc
    24001 tccatcttta gcatttctta ctactattct tccagtctct acttgattta tatcaaaagc
    24061 atgaagtggt tgaccaagct ctaacattac gaagtttgtt atatcgacta tgttacttat
    24121 aggtcttact cctgcttctg taagtcttct ttgcatccaa tatggagaag gttctatttt
    24181 tacatctgta accattctag caacatatct tctacataaa tctggattat ctatctcaac
    24241 tttgaaatct atctcttcgt cactttcttt tacttcgatt tcaggatatt ttacttttgt
    24301 tcctatagtt gcagctgctt ctctagctat acccatcatg catttacaat caggtctgtt
    24361 tgaagttaat tcaaaatcta ttaaagcatc ttttaatcct aaaacatctt ttatatcttt
    24421 tcctaattca taagaatctt cgtggtctaa aatatatata ccacctcttt tatactcctc
    24481 aatgtaatgt tcatctatac ctagttcagc tgctgaacac atcataccat ctgataattc
    24541 acctctgaaa tcagtctgtt tgatttcaac tcctccaggt aacttagaac catttacagc
    24601 tactggaata taatctcctt ctgatatatt tgtagctcct gtaactattt gaacaacttt
    24661 atctccaata tctactttag ttacaaccaa cttatcagca tttggatgtt gttttatttc
    24721 taaaatcttt ccaaccaata tattttctat ttcttcacca taataatcta ttgtttcaac
    24781 tttagttcct gtcattgtca ttttatcagc gaactctttt acatccatgt ctatatcaac
    24841 atagtctcta agccatttta aagatactaa catctacata ccctcctaat taaaattgat
    24901 ttaagaatct catatcattt tcgaataata atctaatatc atctatttcg tatttaagca
    24961 ttgcaagtct ttcaacccca actccaaatg caaatccact gtaaacttct gggtctattc
    25021 cacaatttct aagcacattt ggatgaacca tacctgaacc taatatttct atccaacctt
    25081 catatttaca cattgggcaa cctttaccac cacatttgaa acaagtaaca tcaacctctg
    25141 cacttggttc tgtaaatggg aagttgtgag gtctaaactt agttttgata tcagaaccaa
    25201 acaatttttc aacgaagata tccatagttc ctttaaattc tgccatagta acatcttttc
    25261 caacaacaag cccttctatc tgatggaaca ttggtgagtg tgtagcatct ggcgagtctg
    25321 acctaaaaca tctacctggt gcaattactt ttattggtaa ctcttgactt ctcatagttc
    25381 ttacttgaac tggagatgtt tgagttctaa gtaatacccc atcattgata tagaatgtat
    25441 cgctcatatc tcttgatgga tggtctttag gagcgtttaa tgcgtcaaag ttgttttcaa
    25501 cagtctcaac ttctggccct tctgctatag agaatcccat tccgataaat atatctgtta
    25561 cttcatctat aatttgagtt attggatgct tcttcccaac ctttaaaact tttcctggtt
    25621 gagtaacatc tatcacttcc tcttttaatt ttctttcttt ttcaatagca tttatttctt
    25681 cttttttaga atttatgcta agttcaatgt tttctcttac ctcattggca accttaccaa
    25741 ctactggtct ttcttcagca gataatttac ccatttcttt aagtatggca gttatctcac
    25801 ctttttttcc taagtactta actcttaaac tttctacact ttctatgctt tgtgcttctt
    25861 ttatttcaga caaagctgct tcacgtaaag caagtaattt ttcttgcaca catatcaccc
    25921 tttcgttttt ttgtataaaa aaagcccctc aatccctgtc tagggacgaa aggctatagt
    25981 ttcgcggtac caccctagta gtttaacaat ttaaaaatca tcaaaccacc tctaacaaac
    26041 ttaacgcgtt tttgacgtca aagcctactg taaaatttca gctctgttac tccagaggga
    26101 acttctcatg acctcttaga aaacactccc agcctaggtg tttatctctt tactaagttt
    26161 aatcaaaata ctttcctctt tcattgcatt tacaatatta tattgtttat tttagtatta
    26221 tatcatttcc attcaaatta tgtgactatt tttaatatat atccaataca taattaattt
    26281 aattattttt tatataatat atatctaatg cataattaac tcaattattt tttatataat
    26341 atacacctaa tgcataatta actcaattat tttttatata atatacatct aatgcataat
    26401 taactcaatt attttttata taatatacat ctaatatata attaatttaa ttattttttt
    26461 aatatatatt gactattttt gatactctac tatttaattc aagattataa catgacatta
    26521 tatttattac aatacattaa attaagtatt tttttatttc atacatcagt atagcagagc
    26581 ttatcgcagc atttaacgac tcggctttac catatatagg tatcttaacc aaaatatcag
    26641 actttgatac aagttcttca tttattccat ttgcttcgtt tcctatcacc aatgctactt
    26701 ttgagccata atctattttg tcataaaaat tttctgtatt taagtaactt gaaactatat
    26761 taaaatccaa tgatttaagc atgtccacag tttcatcttg tgaagcatca attatattca
    26821 tatcaaaaat agaacccata gtagacctaa ttacttttgg gttgtatata tcgacacatc
    26881 cctttagtgc tattatggca tctactccag cagaatcagc agtccttatt atagtcccca
    26941 tatttcctgg gtcttgtatt ctgtctaaaa tcaatacaaa tttatctttt ttatttatac
    27001 tttcacttaa tttttttttc ttaaatgaaa ctacacctat tattccttga gtattttctg
    27061 tatccactaa ttctttaaag tttttattag tagtcttgta tatctttgtg ttttttttat
    27121 ccaaatcttc taatagtttt acatgttctt tcttattttc aaattcttca ttgataaata
    27181 cataatcaag gtttgccata cattcaagtg caagcattac tattctgtat ccttctatta
    27241 tgaactttga ctctttattc ctattctttg attttaatag tgctcttgta tactttaact
    27301 tttcattatc cttactgttt atatttgtaa tcattttatc taaatgctaa taacttataa
    27361 atttttatat tagcacagcc tccttacttt taatttaact tctctttatc taatcttaca
    27421 aataaatact accttcgatt aaaaaaatag tatcatataa ctattcataa ttatatgata
    27481 ctatctactt tcattgcaat taaatatata ttaaatagta aattatcttc ttctaattat
    27541 ccccttattt ccagatgtta tcgctgttat actagtattt tgaccgatta taactaggat
    27601 acttccggct gtaagttcct catctggaga aggtgtaaca tttatatttt tacctgtttt
    27661 tatagcaagt acagttatct catatctagc tcttaattca agctctataa gtgtcttgcc
    27721 aacccaacta tttggagtta cgatttcaac aattgaatac tctgggtcaa gttcaatatg
    27781 gtctaatata ttatccgaaa ctaaattgtg tgcaactctt actcccatat ctctttctgg
    27841 aaatacaact ctatctgcac caattttata aagaacttta gcttgtaatt catcctttgc
    27901 cttacatatt atcaactcta cacccatttc tttggctata agagtcgcca ttatagatgc
    27961 ccttatatca gaacctattg caactactgc tacatcaaag ttacctaaac ctaatgacct
    28021 taacgcttgc tcatcagtaa catccactat aagtgaatgg gttaccttgt cagatatact
    28081 ttgaactgaa tcttcatttt tgtctattgc cattacttga tgtcctaaaa gatgcatagt
    28141 agacgcaact gaacttccaa atctcccaca cccaatgact atatattgct tcatctcgaa
    28201 acttcctccc taatacacaa tttatataat atctcttaaa ttaattgttt acacatacaa
    28261 aacttatata ctttacacat acaaaactta tacacaatat ataattttat acacacagat
    28321 taatttaatt ttgatttacc tttttatttg tattttattt ccctgttttt ttatttcatt
    28381 aattaatttt gttcattagt tgattttatt tcttcatata attacatcta ggctcattta
    28441 ttttatcaac agatttacag tgctactcta aacccattat aaatttatac tactccttgg
    28501 taacttaacc aaccataatt ttaccttctg catatctgat tggttgactc tttcttctac
    28561 caccagaaag tatagccata aataaagtaa gagaaccaac tctacccata aacataaaga
    28621 ttattattat aatcttacct aaagcattta agtttggagt tccagctaaa cttgaaccaa
    28681 ctgtagcaag tgctgatgta acctcaaaag ctgattgaac taatgtaaaa ttaggctgag
    28741 ttattgaaat aatcaatgtt ccaaaaataa ctgctgatat actgattaca aatataccaa
    28801 gagatttttt tatagttgat ggagctaatc ttctttcaaa agcttctata tctgattttc
    28861 ctgatagaaa acttctaact gtaatgatta aaacagctag tgttgttgtt tttatacccc
    28921 caccagttga tgcaggtgat gctccaataa acatcaaaat tatcattaca aatacactac
    28981 tttctcgtaa agtagctaaa tcaagcgtat taaaaccagc agttcttgct gttactgatt
    29041 gaaatattgc tgatagtact tttcccttta aaggcaagtc cgccatagta gctttttgat
    29101 taaattctat aagaaaaata aataatgcac ccacaaaaat aagtgtagca gttgaaaaca
    29161 atacaacctt agaatgcaca tttaattttg aaaatcttct ttttcttaca atatcaagta
    29221 caacagggaa tcctagccct ccaagcacta taagtgcgca aacagtcatt gatactgtaa
    29281 agttgtttac ataagaattt aacgatgtaa atgggccact tacactgccc atcaagtcaa
    29341 atccggcatt acaaaacgct gatattgcgt gaaatacact ataccatatc cctttagaca
    29401 aaccaaattg tggaataaag acagttgata aaaccagtgc acctattccc tctattgtta
    29461 tggttattat taatacaaat cttgtaagtc ttacaagtcc cgataaatca gcttgattta
    29521 atgactcttg tattaataat ctttctctta gttgtatttt ttttcgtgcc ataagtgaaa
    29581 acattgtggc tacggtcata aatccaagac caccaatttg tatcaatgtt attataacta
    29641 tctgtccaaa taaactccaa taagttgctg tatctaccat aactagacca gtaacacaaa
    29701 ccgctgaagt agccgtaaat agagcattta ataagcctat actctctcca ctctgcgacg
    29761 ctatcgggag tgttaacaac aatgctccga ttacaataat cgcagcaaac ccagatacca
    29821 ttatttgagt aggacgcatt ccatctgcgt agagaataag tcttctaact attgttttca
    29881 actttcttta tacctcctta acataaaatc actactgtta agacccttaa tttgtttatt
    29941 tttagagctt tcctctaaat tattattcta ttatagtata aatattgtat aaagacaatg
    30001 atagtaattt ttataatttc tgtttggaat attttaaatt tttatatata tcttttaatt
    30061 taaattgcac caaaaacatc tatatttttt acatataata ggtctaaaac tatcatagct
    30121 tttactattt tgtacttaaa atttatttat cattttacca gtataaattt catatacttt
    30181 tttgaagtta aattttttat attatttata atcttatcta tatctttatg ttaaat
//
LOCUS       DAGSWI010000043        30019 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.043, whole genome shotgun sequence.
ACCESSION   DAGSWI010000043 DAGSWI010000000
VERSION     DAGSWI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.043"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(50..1138)
                     /locus_tag="MJF80_001702"
     CDS             complement(50..1138)
                     /locus_tag="MJF80_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280370.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            1263..1991
                     /locus_tag="MJF80_001703"
     CDS             1263..1991
                     /locus_tag="MJF80_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBZ0280371.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(2193..2684)
                     /locus_tag="MJF80_001704"
     CDS             complement(2193..2684)
                     /locus_tag="MJF80_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280372.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(2695..3138)
                     /locus_tag="MJF80_001705"
     CDS             complement(2695..3138)
                     /locus_tag="MJF80_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280373.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(3128..3967)
                     /locus_tag="MJF80_001706"
     CDS             complement(3128..3967)
                     /locus_tag="MJF80_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280374.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(4186..5571)
                     /locus_tag="MJF80_001707"
     CDS             complement(4186..5571)
                     /locus_tag="MJF80_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0280375.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(5744..6487)
                     /locus_tag="MJF80_001708"
     CDS             complement(5744..6487)
                     /locus_tag="MJF80_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBZ0280376.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(6507..7769)
                     /locus_tag="MJF80_001709"
     CDS             complement(6507..7769)
                     /locus_tag="MJF80_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBZ0280377.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(7953..8951)
                     /locus_tag="MJF80_001710"
     CDS             complement(7953..8951)
                     /locus_tag="MJF80_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBZ0280378.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(8935..10446)
                     /locus_tag="MJF80_001711"
     CDS             complement(8935..10446)
                     /locus_tag="MJF80_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280379.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(10500..11462)
                     /locus_tag="MJF80_001712"
     CDS             complement(10500..11462)
                     /locus_tag="MJF80_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBZ0280380.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(11510..12400)
                     /gene="rbsK"
                     /locus_tag="MJF80_001713"
     CDS             complement(11510..12400)
                     /gene="rbsK"
                     /locus_tag="MJF80_001713"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBZ0280381.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(12442..13455)
                     /locus_tag="MJF80_001714"
     CDS             complement(12442..13455)
                     /locus_tag="MJF80_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBZ0280382.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(13684..14661)
                     /gene="bioB"
                     /locus_tag="MJF80_001715"
     CDS             complement(13684..14661)
                     /gene="bioB"
                     /locus_tag="MJF80_001715"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBZ0280383.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEF"
     gene            complement(14867..15130)
                     /locus_tag="MJF80_001716"
     CDS             complement(14867..15130)
                     /locus_tag="MJF80_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280384.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(15200..16273)
                     /locus_tag="MJF80_001717"
     CDS             complement(15200..16273)
                     /locus_tag="MJF80_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBZ0280385.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(16521..17279)
                     /locus_tag="MJF80_001718"
     CDS             complement(16521..17279)
                     /locus_tag="MJF80_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280386.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
     gene            complement(17276..18193)
                     /locus_tag="MJF80_001719"
     CDS             complement(17276..18193)
                     /locus_tag="MJF80_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280387.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(18193..18393)
                     /locus_tag="MJF80_001720"
     CDS             complement(18193..18393)
                     /locus_tag="MJF80_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBZ0280388.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(18398..19492)
                     /locus_tag="MJF80_001721"
     CDS             complement(18398..19492)
                     /locus_tag="MJF80_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0280389.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(20002..20142)
                     /locus_tag="MJF80_001722"
     CDS             complement(20002..20142)
                     /locus_tag="MJF80_001722"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBZ0280390.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDEN
                     GE"
     gene            complement(20452..21759)
                     /locus_tag="MJF80_001723"
     CDS             complement(20452..21759)
                     /locus_tag="MJF80_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBZ0280391.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(21824..22303)
                     /locus_tag="MJF80_001724"
     CDS             complement(21824..22303)
                     /locus_tag="MJF80_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBZ0280392.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(22350..23213)
                     /locus_tag="MJF80_001725"
     CDS             complement(22350..23213)
                     /locus_tag="MJF80_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBZ0280393.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(23213..23968)
                     /locus_tag="MJF80_001726"
     CDS             complement(23213..23968)
                     /locus_tag="MJF80_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBZ0280394.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(23995..24483)
                     /locus_tag="MJF80_001727"
     CDS             complement(23995..24483)
                     /locus_tag="MJF80_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0280395.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(24501..24902)
                     /locus_tag="MJF80_001728"
     CDS             complement(24501..24902)
                     /locus_tag="MJF80_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBZ0280396.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(24905..25402)
                     /locus_tag="MJF80_001729"
     CDS             complement(24905..25402)
                     /locus_tag="MJF80_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0280397.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(25426..25830)
                     /locus_tag="MJF80_001730"
     CDS             complement(25426..25830)
                     /locus_tag="MJF80_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0280398.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(26060..28627)
                     /locus_tag="MJF80_001731"
     CDS             complement(26060..28627)
                     /locus_tag="MJF80_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0280399.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            28793..29536
                     /locus_tag="MJF80_001732"
     CDS             28793..29536
                     /locus_tag="MJF80_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBZ0280400.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHKWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT         9638 a         5540 c         3057 g        11784 t
ORIGIN      
        1 taagcgagat taaattcagt taatctcgct taaataatgt ctaaatttat tattccaaaa
       61 aatcatcaaa atctaatgaa cctgattctg caaaatcata taatgcattt tgctcaaaga
      121 aaaatttctc ttcaaattta atatagtcgc tataattaga ttgacttatt tcattgatat
      181 aattgcaatc attttcgtta taagtaccaa taaaaaactt atttcccatt aagtaatata
      241 tagctttcat atcattattt attatatata cattttcttt tacatggtca ttattaaaat
      301 taaatctagt aaagaatata ccttcattca tattactttt taacagaaat ggattttctc
      361 tataaaatac tcctgcaaat tcttcaggtg aatttacact gtaaacactt acaaattcac
      421 ttttagaaat ctcatcaaat acctcaaaat catacatagg ttctttatct gtcactaata
      481 gagaattaat tctaaaacca gtttcataac acttacttat gcaaaaagca attttacata
      541 tataatatcc atcattatct tcatataaag cttcacttat atatctgcct tgacctttat
      601 cacttaccac gtttttaagt aatgttccat ttatattagt aatgtgcata tttgcaatct
      661 cgtcacttcc agaaaagtac tttaaacttt ctctatccca tgctataaat ctcatggtca
      721 tatagtttat aaattcaact tcctcgtcaa ctcttttaca tattttattg tataattttt
      781 ctatagttaa atctgtttta tagtttttta taatatcaat atactcacat ttatctcctg
      841 gcaatagctt atcataataa agattcttat taccaaaata agatactaaa aataaagatt
      901 catccttcgt taattcaact ctatctgaac caaatcctcc catcaatctt tcttcttcca
      961 tataagcttc ttcttgtgtt gggttattta aactcacata atccgctaat cctaatccct
     1021 ctgcatctaa taaaaaatat tgacaaaagt gattgttttc atcatctatc caatttatta
     1081 taagacctaa actgcccata agtctagaat ttgtagcctt tgcaaaatta aaattcatat
     1141 agtcacatcc tttaaactct aaaatacttg ttttttaata tataaattat tatacatata
     1201 ttttaacaac ttagatacaa tttataatat aatataagga aatgattaat tgaggtggtc
     1261 ttatgaaaaa tatagcagct ttttttgata tagatggtac tctttataga gactctctta
     1321 tggttgaaca ttttaaaaaa ttaattaaat acgatataat tgaccaaaaa aattggtata
     1381 aacatgctag agacactttt atggattggg ataaaagaca aggtaattat gatgattatc
     1441 tattagaaat atgtgacctt tatgttgatt cactaaaagg attagataaa acctgtatca
     1501 actttacaag tgaccaagta attaaattaa aagcagatag agtttataaa tacacacgtt
     1561 caaggattga gtggcatcta aatcaaggac acatagttat atttatatct ggtagccctg
     1621 gatttcttgt tgaaaaaatg gcaaaaaaat ataatgtaac cgattttcta ggaagtgatt
     1681 atgtctttga aaacaatata tttacaggca cagttattcc aatgtgggat tctataagta
     1741 agaataatgc tataaacgat tttgttgtaa agtatgattt agatttatca aactcgtatg
     1801 cctatggaga tactaatggt gatataaata tgctaaaaag agttggaaat ccaatagcaa
     1861 taaatccaac aaaggaactt ttatctcaaa tatcacttga tgaggaaatt tctaaaaatg
     1921 ttgagataat agtagaaaga aaagatatta tttattctct atctacagat gtaaatatag
     1981 tggatattta atttatacta tctattttaa aaacttatat tatatctaat atttatatct
     2041 aacatataaa agctttgact aaaaatcaaa gcttttatat gttgcactaa gaatatatta
     2101 aatttagctt tatgctttaa tgtatattca tatatatcat ttacacttat ctaaactaca
     2161 atttcattaa aattaaatat aaaaatcaac tattattttt taaatagcat ctttcttaat
     2221 ctattattgt aataacctat aaaagaactg taaacatagt catatactat aaaggctatc
     2281 tcaaatgaac ctataataaa tatgtttatt ggaatataaa ctattgttct taataaaaaa
     2341 tacaatataa gtatcattat atttgcaaat actaatttca taaaatactc aatatatata
     2401 gacctatctt tttctataag atattttaca ataccatata ttccaaaggt aagtgaatat
     2461 acaatccatt gagacttact tgccatcact ataaatccta aaactataga acctatataa
     2521 aaagcaattc cactcttaaa gccccattcc attattacaa ttgatactaa aagtgatgct
     2581 aaccccataa taaacaatgt atttacagga ataatgtttg taagagtcaa caatatcaca
     2641 tttaatgcta ataaaactcc tccataagca acttttatac tcatatttat catcctaaca
     2701 acaagttatt aaatctccac caaaacattc acaacatgaa tctaaacacc aaagtttagc
     2761 acaattttca caacaatcgt cacctagaca acaacagcaa tcatcacagc aacagcaaca
     2821 acatccatct aaatcattac gtcttcttct attataattg tcagccctat tgctataatc
     2881 atttctatat tgtgtataac ttctaagtgc atcactatat tcactatttt caggctccat
     2941 aaattttgct ctttttaaga agtcttcacc ttcttcataa tatcctatgt tcattgcaga
     3001 aaaaccagtt aaataatacc actctgcaca tttgtcagtc aaagttttta aaaaatcata
     3061 tgctttttta aattcactat tttctatgaa tgatcttgct ctcataaaat tttcattatt
     3121 ataagtatta ctgtacattc tttccacctc tactttctaa tatattaata tatcttaaat
     3181 aaacaccaga ataaattata ttttctatta ttccaacatt tttatttaga tttagattat
     3241 caattcccct agctaaaaat cctaatgatg ttactattat tttttttact ttttctttta
     3301 actcatcatt tttatcaata tactctataa atggattata tcttcctttc ttaaaatctt
     3361 catctaaatc ttcataggca tctagtagat atatatactt acctatatta aaacctatca
     3421 ttcttaagtt tctctcattt tcatcttttt tatagacaaa tacttcaccc attatatttc
     3481 caaatatact agatacttca tctatattta tatttttatc tttttctaac ttatttaatt
     3541 catttagttg ttgttttata tactctgctt tttcaggata cttttcatat gctaatttta
     3601 atttgttttt atatatataa tatgccaatt tatcttttat gcgtttatca tccattaaat
     3661 tatcttccaa tttataatac gttaaaagaa tattcatact tgctgcatat tctgtaatat
     3721 catttgtaat tacctttttc tttttaaatg gatttactat acacacttcc tctgtactta
     3781 tagactctgg gttatatact gctgttaata aaacaattaa aaatgttata tcatagttca
     3841 aagaaaacct agagatttct ccatgatttc tttttaagta tttacatagt ccacaatagt
     3901 aagctttgta ataatcatat tctctaaatg ttaaatccat tttgtttatt ttaacatatc
     3961 caaacataaa tactcctttt tgtactattt tttatttaga ttatatatag tataacactt
     4021 tttatcaaga ttatataatt tacagtattc taagaaactt aaaaaattcc ttctaatcat
     4081 tgtatcttaa atttaaaata attagaaggt tgggttaaaa ataatattaa attatatact
     4141 tatattaact catacaagtt agttatgctt atatcaattt aagttttaaa taattggtat
     4201 atatttaaaa ctacctacag tatccttcaa ttcttttttt gctttgttta taaccaattc
     4261 atcaagcatt acatcattta ttgaacatga tatagattta gatgccaata acattgcctt
     4321 aaatcccata tcagaacctg cactggcaca agattgccat gtatgggcag aaataccaat
     4381 aggccaaact gaacaagaac actgtgctgt aggaattatg tagctaacat ctccaatatc
     4441 taatgataat gatatacagt tattatgagt aaatgtatca tccgtaactt catcatgcag
     4501 attcatctta gtaatatcct tatttccact taatacacta gaaacactac ttcttttatc
     4561 tatagacact gtctcagcta atttatctgc aaaatagtag tcatcttcat tatagttttg
     4621 cacacctact aatttcatat tttgtgatag aatatctgtt aatattgtat ttggaatata
     4681 gtcatatact ccacttataa tgtcatattc cataatagta ccagtcatta atgctgctcc
     4741 ttttgctata tcaacaatcc taccaaatac atctcttaca tgctccattt taactcctct
     4801 tatgtaatac cacacttctg caaaatcagg aactatattt ggcatttttc caccattagt
     4861 agttacatag tgtactctac aattatcaaa tatatgttct ctcaaataat tacaacctat
     4921 attcataagt tctactgcat caagtgcact tcttccgtta taaggagctt gtgctgcatg
     4981 ggcactaatc ccattatatc taaatttcac agagtaatta gcaagtgttg gtattcttat
     5041 tggagtattt atatcaaatg gatgccaact aaaagcacaa tcaattccat caaaaaaacc
     5101 tttttttatc atcaatactt ttccagataa atcttcttct gctggacatc catagtattt
     5161 tatagtccct ttcagctttt ttagtttgat taattccttt actgcaatta cagctcctat
     5221 tgaacctgta ccaagtaagt tatgtccaca tccatgacct gcaccaccat ctacaatagg
     5281 ttctttagta gtggatactt tttgagacaa accatctaaa gcatcatatt ctcccaaaac
     5341 tgctataact ggtctacctt ctccataact agccacaaat gctgtatcca taccttctaa
     5401 tccctctata ctaaaaccat gtttttttaa acattctttt tgtaaatttg aagcatagta
     5461 ttctttatac tgaagttctg gatttttcca tatcttatct gatatatctc ttaattctcc
     5521 ctgtatctca tctatatatt ccatcaaaaa ctttttattt aaattatcca tattaattat
     5581 ataaacctcc ctgtttctaa taacttatcg attatcagcc aaaatgacta attttaactg
     5641 atttaaaaat attttctacc atacaaccat tttatacaat aaaacctctt gacttgtaag
     5701 tttcatttta tcaagtaaga ggttttcaag ttttataaaa tttttagcat gttaaccaat
     5761 caagcatatt aacccataat ttattatagt atttccaatc agtaaattct tttggacccc
     5821 agtgaggtgc acagtcagaa ctaaagatag cagattttcc ttttccaaac tcgccaacag
     5881 ctacaaatgg gtctcctcca atagttgcta aaacaggaca attatctcta gctactgttt
     5941 tgttatatcc taaaaacttt ggccattctg ttggtatacc ttttaatact ggatgttcag
     6001 agtcaataac ttctggaatt attcctgctg gtttttcaac tctatcatct ttatctatca
     6061 ttgttattgg taatacatct ttgatagcag tttctccaaa tctagctttt gcatctattc
     6121 ctgtaaatga catatatcca cctaccatga ttaacgcccc accattattt acatactctt
     6181 tgattagttc aagtctatct ggattactat tgcaatctat aaaagtacta ttagataata
     6241 agaacgtatt agaacctata tcagataata ttatacagtc atactcattt aactcttcta
     6301 atttatacgg aaaactatct gctgctacat gtgcaggcat atgaactgtc tcatatcctc
     6361 cagattctat tgcttctttc aaccacttta cagcttcttc ataatgagtt gtaacaaacg
     6421 tatcaaaccc ttttatatga gttatatttt ttacccaaga ttcccctact attaatactt
     6481 ttttcattta tatattcctc ctacttttat aagtttaata ttagacttgc atacataagt
     6541 atactatcta aatactcctt aatttcaaca tattcatcaa ttgaatgaca ttgttctatt
     6601 gaacctggcc ctaatataag tgttggtatt tcagctaaat ttttcattac tctcgcatca
     6661 ttgccagctg ttgaaccttt tatctctaat ttttttccta gagtttcttc tagtatttta
     6721 tgtgcattca ctacaaaatc agaatccttg tccatctcaa aacctaaccc tgattgatat
     6781 atgtttatat ttggtttatt atctttaagc cataaatctc cattagccct tgtcattatg
     6841 acatttgtta catcatttac aacttgttca taactcatta tatttggcaa gaagtgtaca
     6901 cataaattaa atacacactt atctggcaca gtagaacctg ctgttccacc atttattaca
     6961 cctaaattta ttgtaggtga aggtaatagt ggatgtttat atatcataag ccagttatgt
     7021 tccaactctt ttatatcttg aagcaacaac atagccttct caattgcatt tacaccctcc
     7081 cacttactac cacagtgaag agacacacct ttaacctcaa cttcaaagaa tacaaatccc
     7141 atatgtgcaa ctattaagtt ttgttctgat ggttcacaaa ttatgcaaca atctccatca
     7201 ataccattca taactgcatt tatggtacca tttccgccac cttcttcatc tactactgac
     7261 attattttta cattaccagg tacatttaga ccactatctt ttataagctt tacagctaat
     7321 attgatgcta taagtccaga tttcatatca gcagttccaa gaccataaag ttttccatta
     7381 tcttctgtag cacgatatgg attatatttc cacttactta tatcacccgg tggcatagtg
     7441 tctacatgac cattaaatac aattgtctta ccaggtaaat catcactata cttacaaatt
     7501 aaattatatc tatcttgata attgtgacct agatttcctt ctttatatat attttttgct
     7561 tttttaatta attcttcgct catttcttgt ctatccacac taaaacctat gtaatttgca
     7621 agtttttcaa tatattcctg tccttctttt tcaaatccac ctagtattcc atgaccaaca
     7681 tcttcagtct ttatacttac aagttctttt aaataatcta tgtattcttg ttttttacta
     7741 tttaatgtat ctaagagaat ttttttcata tttcctccta agatttattt ttacatttaa
     7801 aatttcaagt ctaatctcat ctatttaagc catagtaaac tttttcactt agcatattct
     7861 cattcaatgt ataaagatgt atgcaaatta aagtataaaa atatatacaa aaacttaata
     7921 atttttatag tttataagct aaaatttaat tactatttag atttagctga tttttttaat
     7981 tttatattat ttatccatat agcaaataac attaccaaac cttttataat catttgatta
     8041 tatgaagata cacttaataa gtttaatcca tttcctaata ttcctattat tccgacacct
     8101 atacatgtac ctactataga accagaacca cccatcatat tagtaccacc aagcacaacg
     8161 gctgctatag catccaattc tgctccatct ccaacagttg gtgaacctga tgccaaacgt
     8221 gatgatagta ttatcccagc taatgctgtt agtagtccag ctagtgcgta tacttttact
     8281 ataactttat ttacttttac tccacataat ctagcggcct cttcatttcc acctactgca
     8341 tgtacatgac gtccaaaatt agttcttttt aatagaaaat gtcctgctat tacaactata
     8401 atcataatta taactggtat tggtattggt ccaatatatc ctctaccgag catcttaaat
     8461 gcatctgtat ttactgatat aggtcttcct cctgtaaata caaagcttgc cccacttatt
     8521 atcgttaaca tggctaaagt tacaatgaat ggaggtagtt tttgtgttga aataattgca
     8581 ccttttatga ctcctaatat tgtccctatt gctaatgcaa gtatcatacc tacccatata
     8641 ggtgcattat aattcactac tgttgtagcc caaaatgtac ctactaaagc aattactgct
     8701 cctacactta aatctattcc tcctgtaatt attacaaatg tcatacctac tgcaagtatt
     8761 gcatttattg atatttgtct tccaacatta attaaattat ctaaagttaa aaagttagat
     8821 gaagctacac tcattattat aaaaattatt acaagtccta cagctgcact cccttggtct
     8881 ctaaatacac ttgttaattt tgtcaataat ggagaagatt tttcttcttt atgcttagtt
     8941 attgaactca ttaattttac ctccgaaagc caattttaat acattatttt caaccatttc
     9001 ttcaccttct aaaaatccac ttatatgacc ttctctcata accattattc tgtcactcat
     9061 tgatattaat tctggtattt cagaagatat cattattata cttacaccac tctccactaa
     9121 atctcccatt agattgtata tctctgcctt agcatttaca tctatacctc tggttggttc
     9181 atccataatc aatatatctg gttgtaataa aagccatctt gctattatag ctttttgttg
     9241 gtttccacca cttaaacttt gtataacttg attcatatgt ggtgttttta tctttaaagc
     9301 ttctatgtat ttttctaaaa tctctttttc cttttttcta tctataattg atttaaactt
     9361 agatatcttt tttaacccag ccatagttgt attttctttg acactcattt gtaaaaacaa
     9421 tccagtacct tttctatctt ctgttattaa tcctagtcca ttttctattg catctgatgg
     9481 agttttaaac tttacttctt cattgtttaa atatattttt ccagaatcat atgttctaag
     9541 tccaaatata gcttctacta tttcacttct cttacttcca actagtccac ctattcccaa
     9601 tatttcacct tttcttatat taaagctaat atcattaaaa acattgttct tagttagatt
     9661 ttccactcgc ataatttctt cttttatttc tacttttctt tttgggaaaa attggtctag
     9721 ctttctatct atcattaaat taactaattc ttcctcagac gatacatctt tggtatttaa
     9781 agtcgaaatc gttttcccat ctcgcattat agttattctg tcacaaatct caaagagttc
     9841 ttccatccta tgggatatat atattatact aactccactc tcttttaaat catttataag
     9901 tctaaaaagt atttcagtct cttttttact tattgaagaa gtaggttcat ccattattaa
     9961 tactttagtt tctgatgata aaactcttgc tatttctacc atttgttttt gagctatact
    10021 aagctctttg actaaactat ttggatttat atctattccc atattatcga aaatcttttt
    10081 agcctcttca ttcatttttt tgtcattggt taagactttg ccatttattt cttttcccat
    10141 aaaaatattt tgtgctactg ttaattcgtc aaaaactgtt agttcttgat atatggcatt
    10201 tatacctatc tttttagaga tatctgggga tatattctct atttctttgc cttcaaaaat
    10261 aaattttcca gaagttttcg aatgtgcacc agttaaaatc tttatcattg ttgatttacc
    10321 agcaccattt tcacccagta gtgcatgaac ttcgccatga aataactcaa aattaacatt
    10381 atctaatgct ctgacaccag gaaattcttt agcaatatta cttaacttca gaattatatt
    10441 actcataatg acactccttt ttaaatagga tttctttcaa ataatctcat tttattttac
    10501 taattcttaa agtcttttat attttcctta tcataaagta aagctggtac tggtatattc
    10561 ttttctacct tttccccttt tatagctttt actactgctt ctacagctaa acgtcccatc
    10621 tctttaggtt gttgttgaat catagcaaga atagttccat ctgttattcc acttgatgct
    10681 tcttcagtta agtcaaatcc tacaatctta acatccttat tttttgcaga ttcaagtgca
    10741 gctttagctc ctagtacact attttcagct gttgcatata ttatatctac atctggatta
    10801 gattgaatta agttttctac tgtattaaga gattcttctc tgtcatatcc tggttgtgaa
    10861 tttaatatct taacatttgc tgaacctttt aatgcatctt taaatccatc tattctctgc
    10921 atttgtattt gagattttaa atctgtcact attgctattt ctgactttcc acctaacttt
    10981 tcatctatgt attttttagt gtattcccct aattttttac cagcatcata actaactgtt
    11041 cctacataac ttgtaatatt ttcagtattt aatacagcat ctactgtaac tactggcatt
    11101 ttttcaaatg ctccttctac tgctggtact atagccgaat tatcagttcc aagtactatc
    11161 attccatcta cattttgttg tataaaatct tctattgctg atgtttgctt agctacatct
    11221 tggtctgggt catttactat aactttcaca cctaattctt tagctttttc ttttattccc
    11281 tcatcaatat ctataaaaaa ttgatgttga agagttattg tagatactcc aatagttaaa
    11341 tctttcttgt ctgaaccacc tttattatct gatttacttg gtccttccat tgaacaccct
    11401 gttaacattc ctattgctag tattccagca attcctaaag ataaaatttt tctcactctc
    11461 ataattaacc tccattttta atagttttaa atttgaacaa attagatttt tatttattat
    11521 ttaaatactt ttcaacatct tctttatagg gcataccaga ttgtgcacct agtttagtta
    11581 cagaaagacc tgatactact actgcatatt ctactgctgt atataaattt ttcccttctg
    11641 ttatagccac tgccaatgct ccattaaatg tatctcctgc tcctgtggta tcttgaacat
    11701 caacacttat acaagataca tgcttgattt cctcaccatc ataaaatcta gctccatttt
    11761 ttccttctgt tattacaagt ttatttggat atttttttaa tacttcttct atcccttcat
    11821 ttgtgtcaaa aactatctta tattcatgtt catttggtgt taagtaagtt actttttcta
    11881 ttatatcttc atttagtttt actgctggtg ctggatttaa taaaacccta atcctatttt
    11941 caaaacagaa attcactaca taatttattg tttttaatgg aatctctaat tgtagcaata
    12001 ctatatctgc attctttatc tcttcttcat tctttttaat taattctata tctactagct
    12061 cattagtccc tggtacaaca acaatactat tatcattttc acataatgtt ataacagcaa
    12121 caccagaact tttgtgatta tttctggata ctaagctata atcaacttta ttcgttctaa
    12181 aatttcttat taactcctct cctaagccat cttcccctat gcatgatatc atttttacat
    12241 ttgctcctag tttagaagct gagatagctt gattagcccc ttttcctcca ggtgatgtaa
    12301 aaaaactatt acctaatact gtttctcctt tttctggtct tttatctaca gaacatacca
    12361 tgtccatgtt tacacttcct atgacaacta tgtttcccat tacattactc ctttacatat
    12421 ctatttatgt ctctcgtgct gttattcact ttcttttgtt gaatctctta tgaccaattc
    12481 tggtttgaaa atcaattcat caaacgattc ttttttatct cctcttaaac aatttatcaa
    12541 tatttcactg gctttatatc ctatatcata agacggctgt tttactgtgg taagagaagg
    12601 gcttattaga cttgatgttt gaatatcatc aaatcccacg acacttatat catttggtat
    12661 acttaatcct ctttctttaa gacctcttat gacacctatt gcaatcaaat catttgcaca
    12721 aaatattgca tcaaaatcta tcttttctaa attttttata aattcttgtc cccattcaat
    12781 cttatatttt ccttcaaata cataagcagg attgtagctt aaattgcact cgattagagc
    12841 ctttttatat ccttctaatc tatctactgc tatttcattt tgaagtggac cacttaggta
    12901 tattattttc ttatttccaa tatctaataa atgttttgta gctatatagg ccccttcaaa
    12961 gttatttatt ttaacaattc cttttaaggt ttctttgttc atacttatct tcttatcaat
    13021 caagacagtt ggtattttta tatctttata atttgcttct ttccagtttg tatttgaaga
    13081 tgctgcaaat ataattccat ctatcatcct ctctgatagt gttcttattc cttcatattc
    13141 ttttttagca tcttcatctg tattacataa aattatattg tagccttctt cattagcttt
    13201 atcttctaca cctcttgata catctgtaaa gaatggattt cgtatatctg gtataagaag
    13261 ccctatagta ttagtttttt tagttactaa actttgtgct aatttatttg gtacataatt
    13321 tttttccttc attattttca aaattttttc tcttgttgcc tgacttatat tttcatcttt
    13381 attattaatg acttttgaca cagttgtttt agaaacacca gccaattcgc caatttcttt
    13441 tatagttata ctcaagaagt tcacccccaa aatttaggaa accgttttac ttaaccggtt
    13501 acctagatac tatcacatct ttttttcatt tgcaaccatt ttatataaat tttgatattt
    13561 ttcccttttt tcaaaatatt gcaacttata attcaatttt aatatttaaa ttaatctgca
    13621 aaaaaaaatt aatatacttt tataattatc taaaagtata ttaattaatt taagcagtat
    13681 ttattaaaat tcaaatcctt gctctataat cattttttta tcattttcaa tcccacttcc
    13741 aacagttgtc aaataatcac caacaattgc agaatttact cccccattaa atccaacttt
    13801 gtcatatccc tttaatgata gccttccacc agcatacctt atgtacacat taggaattat
    13861 aaacctaaat actgctgttg tttttagtat ttccattggt tctaatacat cataattttc
    13921 cattggtgta ttcttaatag gatttaaaat gtttattgga aatgatttta catttaattc
    13981 tcgtatttca aaagccattt ttagtctctg ttcttcactc tcattcattc caattatacc
    14041 gcctacacat acatctagcc cagctttttt agcatctttt atagttttta tcctatcttt
    14101 atatgtgtgt gttgtacata tattatgaaa attatctcta cacgtctcaa cattgtggtg
    14161 atatgtagtc acacctgctg acttcaacat tttagcttgc tcataatcta taatacctaa
    14221 agatgcacat aattttaaat tagtattttc cctcaatcct tcatagatat ttaaaatatt
    14281 attaaactcc tttcctgaca tacctttacc acttgtaacc aatgagaatc tatgcactcc
    14341 tttactctcc atctctttag ctctatttaa gatttcattg taatctaata atgaatattc
    14401 ttctacacca gttttataat aagaagactg agcacaaaat ttacagtctt cactacattt
    14461 gccagattta gcattcatta tcgtacataa atctgctttt cttcccataa aatattctct
    14521 tatttcattt gcagatttta atagaatatc aaaatcgttt ttattatttg tatcaaggct
    14581 tattaaatta agagcttctt cataacttat ttctttttct ctcaatactt tattttttaa
    14641 ttttattatg tatttttcca tatatcctcc tatttgtcaa cttaatattt ttatgtagtt
    14701 tacttattat atatagtatt gcatatataa catttattgg tcaactttat tttagacaca
    14761 agtttactat acacaaaaaa atactgcttc aaaacaatat tagtatatag attatacata
    14821 ttattattaa agcagtattt tttattagaa tataattctt actggtttat aaaactatcc
    14881 ttgtcttaac tccatccaag ctttccattg taataacttc taagccttca tattctctaa
    14941 atagccattt taaatccttc atggcttgat taacctgact ttttgttaga ttatttataa
    15001 aatattcttt tctagtttcc tcatactctt tgctagtcaa aaagtttctt agaaaattaa
    15061 acgcaaatac acttggaata ttgtttacat tagccttctt gctgttttcc ttctcatagt
    15121 aaattttcat aaatgcatac tcctttgata cttttagatt tctcttacaa taattatatg
    15181 catttaagtg ctactattct tataatttta ttaattcata ttctttactt cccataccca
    15241 tttcacatgc atagtctatt attctagttc catttacatg aggatgttca tgtttgaaaa
    15301 catcatgacc catttccttg caaatcaaat catagcatgc ttgttcaata gctacagggt
    15361 ctgttgatgc taatattcct atatcatggg caattggtct acctgaccaa ggtacacaat
    15421 cacaaagtgg agttacattc ataacaaaag ttatataccc taccttgtct tttttatttg
    15481 atactgctcc ataagcgtat tctgccatct tttcgacaaa cacatctgag tcactttccc
    15541 attgtatagt tactgctcta gttggacaga cagttggaca ttctccacat ccataacata
    15601 catcagaatc tataactgct tttttatcca ctatacttat agcttttgta ggacaagaat
    15661 taacacactt tccacaacct acacacttct tttcctttac tactggagta acatcagaat
    15721 gttgcatttg ttttcctgct gcacttgcac atcccatagc taagtttttt aatgctcctc
    15781 caaaacctgc tgcttcatga cctttaaaat gactcattac tatcattgca gaagaattat
    15841 aaatttctcc acctattttt acactttcaa aatgtttttt gtctattttt acattctcat
    15901 aatttctact atataatcca tctgcaatta ttactggtgc atttacaact gcataagcaa
    15961 atccattttc tatagcagtt gtaagatggt ctacagaatt tgttctactc cctgtataca
    16021 gtgtatttgt atctgttaaa aatggctttc cttcgcagtc tttaacctta tcaaccactt
    16081 gtctaactgc cacaggactc atatatgtag tatttccctt ttctccaaag tgaagtttaa
    16141 ttgcgacttg gtcatttttt tctattaaat ctttgaatcc tgcttcatca aataatcttc
    16201 ttacattgtt tggtacgttt ttattttgcg aatttgagta taaatcacaa aaatatactt
    16261 ttgacttttc catatctaca cccctttaaa atatttactt atactaattt tatcactttt
    16321 aaagtaattt ttagtatatt ttaacataat taaatattct tatatttatt tgatacaaat
    16381 aatattattt gtattatttt ctacacttaa ttctatatat taaaaatcaa aaagacttga
    16441 aacttaagtc caagtctttt tgatttttag gtgtagtttt taacaacata agattacttt
    16501 accttgtgat aaaggtatta ttaaactggt tttttattaa ataaaataac agctgctagt
    16561 aaacatacta cagaaataag aactgttata ataattggaa taataaattc tggcattgcc
    16621 actttccctg aaatcaagtc tatatttttt gacatcaaga aattaggagt ataaggtgca
    16681 atctgtttag gaatagaaat taaactcaat aaaagagttg ccaccaatac aagtaaaaca
    16741 ctggcaaatg ctggtctaaa tagtacacac ccaagaatta atacactaat atacataatt
    16801 cctataagcc ataaattaaa tgctgaaaat ataatatgat taagatttgc agttggccaa
    16861 aaatacattg tatatccata agcaaataaa aaactcaacc aaaaaccaat tgtcataata
    16921 atcaccgtaa cagagaattt agacaatact atagatgaac gtgataaacc tttagtcagc
    16981 ataataacaa gtgtcccttt agaatattca tttgataaaa tattacaaaa aataattaga
    17041 gttaaactca taccaagacc agagatattg ctaaaaaatt gtgtccaaga atctaaagca
    17101 gttggtgcag cagtaacttt taaagtcggt gcaaaatgag caatcaagtc tggcataaat
    17161 tttgcagaaa taggactact tataccaaat attagaaata aaataattaa actaaataat
    17221 ttataatttc ttaaattctc tttaaattct ttttttgtaa atgctatgta ttctctcact
    17281 ttactacctc caaaaataaa ttttcaagtg ataattctat tagttccatc tttactggac
    17341 aaagattcat ttctgcgagt gtagatataa ctgttttttg tattgctttt atatctttac
    17401 catgcaaaac aatttccata ttagtttctt ctgaatcttt tagtaaggat ttaataccat
    17461 cactagactt aaacttttga atttcatcat tacttgcaaa ctccagtaaa agtctatcct
    17521 ttccgtgcat actttttatt tctgaaagtg taccactaag ggcaatgctt cctttgttta
    17581 atatagcaac atggtcacag attctttcaa catctgatag tatatgtgtt gaaaatatta
    17641 cagttgttga gtctttgatt ttaagcataa tatctaatat ttcttttcga ccaacagggt
    17701 caagtgcact tgttggttca tcacaaataa gtaatttggg gcgtgaaagc aaagcttgag
    17761 ctataccaag tcgttgcttc attccacgag aaaacccacc tattcgtctc ttttcatttc
    17821 taagacccac aagtgataac aactcttcac tccttatctg tatttctttt tttgaaagtc
    17881 ccgtaatttc accacaaaga gaaagatatt caagtggtct catataattg taaaactctg
    17941 gaacatcagg cagataacct acatgacgat tagaacttgt tttcccatag gtcactttct
    18001 caccacaaac atctatagtt ccactatctg gtttaagtaa cccaagtacc attttcattg
    18061 ttgtagtctt acctgcacca ttcttaccta caaacccaaa gactgaccct tctggtacag
    18121 taaaatttaa attatctatt atctttcgat taccaaaacc ttttgataat ccatttattg
    18181 agacaatatt catcattctt cacctcttcc tattgtaaaa tacaaaattg gtcctattaa
    18241 actaataaac agtactacaa taatccatat tgccttattt ccaattttat aatttggatg
    18301 tctcaacaca tgaacaagtg cagtaattat aagtattaaa tctaaaataa taacaggaac
    18361 caaaagtggt aagtattcca tcaaattttc cataatctta ttcctccaat aaagataatt
    18421 tttctataag aaatttgtat tctttgtttt cacttaatac agaaaacatt ggattatttt
    18481 taatagcagc aacaatacct gcttttacga ttgtatcatc acgtgtaata cctgttccaa
    18541 tttcagaaat ccatctgtca agtttattaa aaaagtcatc cccatgtatt ataattgggt
    18601 atatatcacg tgttgctaaa tctacatatt ttcgtaatga tttaatagct ccttcattat
    18661 caccatgtat taaattaatt tgtgcctgtg ttaaatatga agaaagcata atagcaggag
    18721 ataatgtatc aagctcaaat atatctgaaa ttgctagaat acgtctttct atttccttca
    18781 tttgtaattt atctgcattt tgcaaaatac ccactaggtt ttggactaat gatataaggt
    18841 tctgatatgc accaagttga aatgtttctc ttgcttcatc catttggcca ttcatcattt
    18901 gcccctgtgc aagaagtata gattcattta tcatgggaaa attgctgttt tgcaataaat
    18961 caatcacttg tatgggttga tttaacaata tataacaagt tgcctccata ctttttgctt
    19021 gacggcaaat atctatgtca ttacaagttt cctgtatacg aataaatatt tctaatgctt
    19081 cactaattaa tagttcacgc tttttttcat ctacaatatc atagtgatta actattaaaa
    19141 gacccatccg aaaaatcaaa ggaaaacatg aataatattt ttttatgatt tctcttattt
    19201 catccatgac ttcatcaaat ggtttaaccg taaagtcttt gcacagttta ttatacagtt
    19261 tattaatatc ttccttcatc atttgaggtt cataacatat taactcatca acggtaatgt
    19321 tgaaatatgt cgcgagttgc ggtaaaaagg ttatgtcagg atagctttgt ccagtttccc
    19381 atttagatac agaagcttta gaaacgccta tataatttgc aagttcatct tgtgtaattc
    19441 ctttttcttt acgctttaaa atcagcgttt ttgcgatgtt taattcattc atgtcaatcc
    19501 ctccttatgt ataattatat ccttaaacat gtaaagctaa caagcgattt gaagttgact
    19561 tttgacgaaa aaatcaacca cagagaaaca aataaacaag tatataaaat atatatttaa
    19621 atttatcttt aatctctttt gtaaaatatg taaactttgt aaataaaatc catattatat
    19681 aaaactaata gttaatctat ctaaatacag tttttattta tcacgttttt aaatattttg
    19741 tgtaaaacct cttttaatat aaacacctta ttttcatttc tcatttcata ttctttttaa
    19801 aatactgaat ataactcttc gattttccat ttaattcttt tataaagtta ttattcgtat
    19861 ttattcctct aattataaca ggcaactatc atgtattggt atttgtctat tatataaaaa
    19921 tctataaaag aaaagcagtc aatcatttag attaactgct tgtacatata tcttattaaa
    19981 ctgtataaac tataattttt attattctcc attttcatcc caagtcataa atccccatcc
    20041 actatctgga taagtatcaa tagacttttc tctaatcata taaccaactt tataacactc
    20101 ttttgttatt ttattagata gtatacatcc ttctccattt atctaattcc atttaattaa
    20161 cttttctttt caaccttaac ttttttaaaa tcatcttttt taataatttc atacttattt
    20221 tctcttcaac atcatcaaat gagggtgtct caaaattaac tttttacttc ataaggcact
    20281 cttttttgta tgaagtaaaa tatattttca tcatttcaac aataagtaag aggcttatct
    20341 ctattttgag acagccccat ttgatggtgt tgaacattta aataaggttg aactcgtttt
    20401 atctctcttt ttaagatatc tgtaaatttg cacataaagt ctcttcttct tttacaattc
    20461 taatagtttt attaaagcca aagtatatat ctccatattc tttcgtatat gatttattgg
    20521 tactctttca tctactccat gaccaccagt tttatacgct aagaacttag gtccaaacgc
    20581 caccatatct ttaaaccaac ttgcatatgt gccaccaccc atagcaattg tatatccatt
    20641 ttcatctgtt acttcttcat aggcttctaa taatttttta agtactaaat tactctcatc
    20701 cacataatgt aagtctttat gagcaataac ctctattttc caaccattac aaaaaacctt
    20761 tttgaatcta tctattatgt tatttaaaac tatattttta ccatacctta tatctaactc
    20821 acatacgatt ttttcctcat ttacttttaa tattcctaaa tttaaggtag tgtttttgaa
    20881 tacctcatca tattgattaa tccccaatgc atctccataa taatcactac aaaggtattt
    20941 tgaaactaat tctatatctt ctctatgctg aaaatccata ttatcctcaa ctatctttat
    21001 taccatttta gatatagcat tttctccctt ttcaggatta cttgcatgta cagctactcc
    21061 tttagtagtt aactctgaaa taattccatt aataattttt gcagtacaat gagatggaac
    21121 aacattttct ttagttcctc cccttactat tatgtaaggt ttacttttgt caattttaat
    21181 ttctttttct atccttatat gaacccttcc tttttctcca ttaaccactg gatacatagc
    21241 atctggagta aatcccatac ttggaatttc ttcaccttta tctaagtaat atttgataca
    21301 tttcatacct gtctcctcat ttgtaccaaa aattaatctt attcttttat taattgtagg
    21361 atacatatcc tctatgtttt tcaatacatg tataagcata gatacagcac ccttattatc
    21421 tatgacacct cttccataaa taatattgtc aattactgct cccgaaaatg ggtctacact
    21481 ccacccctct cctggtggaa ctatatctaa atgcattgga atccctatta attcctctcc
    21541 ctctccaatt tctgcatatc caatataatt atcaatattt ttaacactaa aacctaaact
    21601 agcacagtaa tctaaagttt tatctaaaga ttttttaggt cctataccaa aaggacattg
    21661 tgaaataggt tcaccttgtt gtgaatcaat ttttatcaat tcttttagaa attctatggt
    21721 tctatttaaa ctattatcta cacttctgct tatatccata aatcaaccta ctttctatct
    21781 ttttaaagcc tgtccttaaa gaacaggctt ttagcattaa aaattatttt tttgtaaata
    21841 ctaattcaca aacttcatca tcatgagcac atttttgagc aaaatctaat ttaacacctt
    21901 gtgcgcagtc aactcttcca taatccccat aacaagctaa tctacaaatt tcttttcttt
    21961 cttctttagt taatccatat tctttccaag cagcatctaa tggacagtga tggaatttta
    22021 aaacgccatt ctcatctgta ttttctacta attccatttc aaacactttt tgtcctttac
    22081 tgttatataa catttctgcc atttttcctg gagtatctgc tacgttatat ttttgacctc
    22141 tcattttacc aaatccatat attgcttctt tacatatatc atctggatta gctcctaatt
    22201 tttcagcttc tttcataaga agatagaacc atactgctct ttctcctgtt gctcctctaa
    22261 ttccttctaa atattccttt tctgtataca ttttttctga catttctttc tcctcctaaa
    22321 attaaattct atattttaat cactgtgaat tagaatattc caatgaatga ccctaccaat
    22381 gctaatacaa ctaaaattac taataactta gttattgtcc acttcttgta tttcattccc
    22441 caatatacga agaatacaac accaagtggt aataaacctg gagcaatcat atttaatgta
    22501 tcttgaacaa caattgcttt accaccaata tcaaatgata gcggagttga aagcttaact
    22561 gtagttgcag ataatgctcc catcataaac attcctagaa ttgaagcacc atcaattatc
    22621 ttattgacaa taccacctga tagaattttc ttaattgaat ctctacctaa agaatatcct
    22681 aaatgaaata agtaataacc ttcgcaaaat agtaatatac taaacattat tgggaaaaat
    22741 gcacctataa cacttccttc tgctccaaat gaagcaccta aagctaagaa tattgtttga
    22801 aatgtaccaa aatctaaagt atctccaatt ccagccaaag gtcccataag accattctta
    22861 acacctgaaa taacttctcc tgggattttt ccttcagtag ctttctcttc ttccatagct
    22921 aaaactgtac cgtgaattaa tcctccccat atagcttgag tattaaaaaa ttgtaagtgt
    22981 ctctttaacg catctactaa atcttctttt ctcttatata acttttctaa agccggagtg
    23041 aaacttgcac aaactgctaa tgcttgcatt ctttcaaaag agttggataa ttctgctgtc
    23101 caccaccatc ttagatatgt ttttgttaca tctttctttg taagtactct tgtcttatta
    23161 acttcagttg gaccttgtcc atttttaact tctttatttt ttatttcttg cattatactt
    23221 cacctaccct tttgtcttct ttcataaagg taattaaaag agctatacaa acaccaaata
    23281 ttgcagctgc agtaattgat atttcaaaat attgaaccat aaaaaatcct ataataaaca
    23341 ttggtaaaaa tttattttta cctatagtaa atattgttgt tgcaaaacct aatgcaggta
    23401 atataccgcc cattactgtt aatccattca ttaaccattg tggtataaca tctaataatg
    23461 attgaactaa atctgctcca aaaaagttac atacaaaaac tattggaaac cttaaaacaa
    23521 acccaaatat taaagaataa agtgttgcac atctccagat gccttttgta ttaccttctt
    23581 cagcatactt atctgcctta tgaaccaata cagcattacc agttcttctt aagttattaa
    23641 ctaatactcc tataacacct aaaggtaccg caatagatac tgcaacttct gcattaacac
    23701 cagtttgaag tgctactggt attgcaataa gagcagctag acatttatct gatggaatat
    23761 ttccccctgc tgcgaccatt cctagataaa ccatttctat acttgcacca acaatcatac
    23821 cagttgttac atcaccccaa agtaatccaa ggctaaaacc tataaataaa ggagacttta
    23881 gtgttgagaa aagggtatac cctgcaagac ctgctgcaaa ccaataccat aatcctgtta
    23941 caattgccaa catcaaaaca gattccattt ataattcctc ctaatttata ttttttattt
    24001 aaatttcttt aacgcatttt catatgtcat agatgattcc tctggtataa cttgtaggac
    24061 tatttcaacc ccattttcat taagttcatt aagttgttct acatcctcat ttcctaatga
    24121 aacagctgta aacaccattt tctttcctgc ttcatttgga atacctccta attgaatctt
    24181 ttcatattta agtccagctt tataagcctt atttgccata tcaacatttt taaatagaat
    24241 aaatatattt ttatctccta aagtgttgtt ttggaatgct tgtcccgcat cttctgctgt
    24301 taaaataact acttctactc ctgatggtgc tgccatttta tatatgtcag ccataaattc
    24361 atcttgacct aagaagtcat caactattat tattttttga gcttgagcga tttttaccca
    24421 ttttgttata acttgcccat gaattaatct gtggtctact cttactaatg ttatttttgc
    24481 cattatattc acctcataat ttatattttt ttaaattctt taattaaatc cacacaaagg
    24541 ttattatttt ttttgacgat tctttctggt aatgtatgta atttatcttc tttgcttaat
    24601 tcttcatcta cgattaaaat tgtctgtaag ctaagtccat ttattgcatc tattcctctt
    24661 cttatagcaa cagctattgc aacacttgat gtagatcctc catataaatc tgatattata
    24721 attttcacat cttctttatc tactacttct tcaacctctt tcataaatac agaaacatct
    24781 ttttcagaac ttagtgataa aacttcatat tgatttgaca atccaaagtt tttatttgtt
    24841 tcttctacta agacttttcc ccaagttcca tggcacagta tgattacctt atatttattc
    24901 attgctaaat aaactctcct ttatagcgat taatattttg gtttaattct acttcttctt
    24961 catcaggtac catttgaatt gatacattaa tattcttttt aataaattca tctattaaaa
    25021 gaaactcttt tttattaatg tatacatttt gagatatttt cttttttcct attcctgtag
    25081 gtattcttcc aacaataagt tttttgatat taatcccttc ttctatcatt ctatttgcag
    25141 tatctaaatt tttaattaat acaaaaacgt tatcatcagc ttgattatta taaaaatatc
    25201 tccttgattc acttacagtt aatattgata aatcaatatt ctttggtgct gccattttta
    25261 tcaaaccttt agcaataaca tcattggttg tttcatcatc tactactact atctttttta
    25321 cacctagatg cttaacccat ttatatataa cttgaccatg tattaatcta tcatcacatc
    25381 tgcaatattt tactaaagac atttttaatc aaactccttt ttctattaca ttgagctaag
    25441 aactttatta atattgttta taccagtttg tcctgcttca atacatgctt ctgctaactc
    25501 ttctaaactt tttccctctc tattaattaa agcttcaagt agcattggaa gatttactcc
    25561 agtaagtgca tgaaacttgc cacctttttt aaggtttaaa cttgcaacat tacttggcga
    25621 acctcctaat aggtcagcta atactaaaac tccgtcacct tcatctaatt catttaactt
    25681 ttctacaaaa tttttattta agtcttcaat attatcattc ctatttagtt taattgctgc
    25741 tgttctttct gctggtcctg caataagttc acagcttttt ataatttctt cactaaaact
    25801 cccatgagtc ataactatta ttcctatcac caatttcacc tcacttttct tgtcactttt
    25861 tatataagca attgccgtgc caacttatgt gtatcaataa caaatggcta ttttacagga
    25921 tttaatcaaa actttttact caattttttt taaaaactta ttatttcgat atgtatcaaa
    25981 agattttttc aacctgtatc aaatctgtat caaaaaaagt aaaaaaaaga tagccttttg
    26041 gctattttta aattttgtat caacttttga atttatctgt atcatattgt gattcaaata
    26101 tatctaatat atttgcatat tccatatctg aaataattat atcgaatgtc tcttctacta
    26161 gcttaaaact ttcttttata actaaatata attctttatt tctactaatt ctttcttcat
    26221 atgatttata aactaaagac tcttttatta ttactctttc aatcatacat gaacaatgaa
    26281 atataaactt tactaaaagt tcatcttgaa ttactaaaga atagtgttca ctaattctat
    26341 taattacttc ttttaatata aaataagcct tttccccatt taaaaaagtc aaagttttac
    26401 taatattatc aattagtaat ctatcaaaat atctaccacc attatctttt tcagcatatg
    26461 taacttccat atctccataa tgtttcttta tagattctat aatattaaaa tataatttct
    26521 ctaatgtcat ttctggagtt agagcctttc tagttgcttc tattacaata ggtgtactta
    26581 ccatatctat tgcttttact tcttttcctg tagcatcacg aataacttta gcaaatgtta
    26641 aaatagaccc catatcagtt aaaattaaaa ttccttttcc tttatcaatt ttattggcta
    26701 tttctacagc ttgtactaaa atatcctgaa ctctatcttc taatcccata ttaattgcca
    26761 ctgcatggtt acaatccagt aaagtattgg ctacattcac catgctagat gctgtagatt
    26821 caccatgcat tatgactaat attgctatac tctcctcatt taatcccaca tacgataaat
    26881 aaagaaatgt tgctaaaaaa attgcttcat cttctggaaa ctctactcca tacattaatg
    26941 acatgttaga taatatttct tttgctatgt tatattcata tgctctttcc ttcataattt
    27001 catctttatt aggatgcttt actatatttc caatctttat tctttcaatt aaattctgaa
    27061 tatgtaaagc tatagctttt attaatttat ctaatccttc tcttttgctc catttatcat
    27121 gcttttgtaa aatatatttt acagtagttg caattgaatc attaacaatg ttgttataag
    27181 ctgtttgatt actactatta tatataaaag tattcattaa attataggaa tacttctgta
    27241 tgtctccatc tataacactt ctaatttgtg actctttaat accttcttgt tgaagtttat
    27301 tccaagtttc ttttatcatt ccataaaaat ctaaatcata ttttttattt tctattaata
    27361 aattttcact ttcaaaatcc ttttcttctg gtaaaaaaat aatatcatct tttccaataa
    27421 aactgaaatt ttgtacaaac tcctcttttc tattatttcc atataatgat tctctaatat
    27481 tatttggtag aagagagagc cttacatata cacattccct tttataggtc atataatcta
    27541 aaaaagctct tgcacaaatt aattgtatat cgctttttaa ttggcctata tttcccttac
    27601 acttatataa aataaatgcc ttaataactt cttttgaaac ctttaactta acaccaatcc
    27661 taacactctc ttctttaaaa aaataacata taagcttcat tctctcttga aagcctcttt
    27721 cctctaaaga cggaatcttt atagttacag gtattcttct taaaaacgtt tccaacattg
    27781 ctgtttctgg gtcttctgta gtggctgcaa ttatcataac tctacactcg tgtaccttat
    27841 tagcctctcc taaccttcta tatatccctt tatccataag cagaaatagc atttcttgtc
    27901 cctctgcaga taatctatga acttcatcta aaaacaatac tccattatta gcttcatgta
    27961 caataccata cttatcttta tcagctccag taaatgctcc ttttacatat ccaaaaagct
    28021 gcgctaataa taattgctga ttatcagaat aatcagcaca gttaaatatg ataaatggtg
    28081 cattatcact atcttctttt atagttaaac tatacttata catatattct gcaaatatag
    28141 atttaccaac tcctgtaggt cctattatta atgtactcaa ccccttaggt ggatatatta
    28201 ctgctgcttt agcctgttct atttgggacc ttaaacttcc atcacatcct actaaattaa
    28261 aaatattgtt ctcttttttt atactctcta aaatatactc atctttgata gaatcatttt
    28321 cactatattc gtttatagag ttaatattat caaatatccc tttatttagt ttctttccta
    28381 aaatactctc aatactttct ttatataagt actgaacagg cttacccttt atctttataa
    28441 taatactttc ttttattaat tgatttaatt ccttactagc attacttcta tttataccta
    28501 cttcagtagc tatatattct gcatcaaatc caaatctaat gccattatta aagttcaagt
    28561 gttcttctgt taaccttttc aagcaaatta aaactttttc tttccttgat agcttctttt
    28621 gtgtcatagt taacctcccc tataagcagt aatactgtta aacatttatt attaagctta
    28681 attctacaat attttaatat ttttgtctaa ttatgtaact attgattttc tttcctattc
    28741 gttttataat ataaattacg ttaccctcat cttttataga ggaggaattt atatgaattt
    28801 tttatcagat ttacacacac actctatagt cagtggtcat ggctatagca ctttactaga
    28861 aaatattaat tattgtaaag aaaatggaat taagatacta ggtacatctg aacatggtcc
    28921 taagatgcct ggttctcccc acaaatggta ctttcacaac attcaaaata tcccaagaat
    28981 cataaataac attataattt taagaggctg tgaagcaaat atactagata ttaaaggaaa
    29041 catagaccta gagccttttg taattcctcg gttagattat cttatcttgt catttcacga
    29101 agctgtgttt tctccaaata cattagagaa taatactaaa gctttaataa atgcaataaa
    29161 taagcatgat aatattgaaa tattaggaca ccttggaaac ccaaactacc caatcgacta
    29221 tgaattaatc attaaattag ctatagaaaa gaatatctta atagaaataa ataattgttc
    29281 tattaaaggc gtgtcaagaa atggtagctc ggataactgt aagtatattg ctacactttg
    29341 caaaaaatat ggtgctaaaa tcatactgac ttcagatgct cacatttgtt ttgacatagg
    29401 taactatgag tattctgaaa atatcttaaa ggaaattaat tttccagatg aattaataat
    29461 gaattatcct aaaaaactta ttaatcattt tcaccaaaaa ggtaaattac tagatgttga
    29521 ttatacaaat atctaagtaa taatacatgc ttactttaac tcccataaaa attttaatac
    29581 tatttatatt atatgaaata aagaaattat aataaagaag ctatttaata aagcttaatt
    29641 acttttatga acttgatgga ttgatatggt ctatgatagc aatagaaatt ataacatttt
    29701 taataaactt agttttatat accttatagt ataaatctaa aaaagcatca tctaaaaagg
    29761 agctgtcaca tgagcaaatt aaatttgttc tatgatagct ctttttttac ttattttaat
    29821 taggtatatt tttaattatt ttatataaag cttatgttct aagtaaaatt atgcataaca
    29881 aacaaaggta taaaaatacc attttttcag ataatattta attataaata ataaatttag
    29941 aatacttctt ttaaatgatg agagtctaga aatttttatg tgtattcttt aaataaaaat
    30001 ttccagactc tagcccctt
//
LOCUS       DAGSWI010000044        29078 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.044, whole genome shotgun sequence.
ACCESSION   DAGSWI010000044 DAGSWI010000000
VERSION     DAGSWI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29078
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.044"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(121..894)
                     /locus_tag="MJF80_001733"
     CDS             complement(121..894)
                     /locus_tag="MJF80_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBZ0280401.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="MJF80_001734"
     CDS             complement(894..1280)
                     /locus_tag="MJF80_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0280402.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="MJF80_001735"
     CDS             complement(1305..1622)
                     /locus_tag="MJF80_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBZ0280403.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="MJF80_001736"
     CDS             complement(1634..1894)
                     /locus_tag="MJF80_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBZ0280404.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="MJF80_001737"
     CDS             complement(1894..2190)
                     /locus_tag="MJF80_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBZ0280405.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="MJF80_001738"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="MJF80_001738"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBZ0280406.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="MJF80_001739"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="MJF80_001739"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBZ0280407.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="MJF80_001740"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="MJF80_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBZ0280408.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="MJF80_001741"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="MJF80_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBZ0280409.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /locus_tag="MJF80_001742"
     CDS             complement(8083..9189)
                     /locus_tag="MJF80_001742"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBZ0280410.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="MJF80_001743"
     CDS             complement(9431..10753)
                     /gene="dnaA"
                     /locus_tag="MJF80_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBZ0280411.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="MJF80_001744"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="MJF80_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBZ0280412.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="MJF80_001745"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="MJF80_001745"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBZ0280413.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="MJF80_001746"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="MJF80_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBZ0280414.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="MJF80_001747"
     CDS             12283..12990
                     /locus_tag="MJF80_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBZ0280415.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /locus_tag="MJF80_001748"
     CDS             12959..13588
                     /locus_tag="MJF80_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBZ0280416.1"
                     /translation="MRKSLKLIKSKSKEEAISKVIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="MJF80_001749"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="MJF80_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBZ0280417.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15174..17069
                     /gene="mnmG"
                     /locus_tag="MJF80_001750"
     CDS             15174..17069
                     /gene="mnmG"
                     /locus_tag="MJF80_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBZ0280418.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTNSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVDKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGDDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="MJF80_001751"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="MJF80_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBZ0280419.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="MJF80_001752"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="MJF80_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBZ0280420.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18864..19637
                     /locus_tag="MJF80_001753"
     CDS             18864..19637
                     /locus_tag="MJF80_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBZ0280421.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19639..20502
                     /locus_tag="MJF80_001754"
     CDS             19639..20502
                     /locus_tag="MJF80_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBZ0280422.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20535..21677
                     /locus_tag="MJF80_001755"
     CDS             20535..21677
                     /locus_tag="MJF80_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBZ0280423.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(22102..23001)
                     /locus_tag="MJF80_001756"
     CDS             complement(22102..23001)
                     /locus_tag="MJF80_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBZ0280424.1"
                     /translation="MSIYGYTIFETVLEQNSFIKAASILNLTPSAVSHAIAKLEIDVG
                     FQLFIRNRNGISLTREAEKILPHIRILLHSNEILEQEINRISRSESGIIRIGTFNSIT
                     INWIPEIMKSFRQEHPNIEIHILQGNYINILEWIETSIVDLAFISNTVLPPHIESIPL
                     HKDRIICVFPKGFVPCNPGYVTIEDIQNMSFVMPKKDYDQEVEKILREYHLNVYSQFH
                     IDTDSAIVAMVEAGFGIAILSELIYKSCVGNVDTYPFVPEHYRVISLAYPNSQYDSFA
                     VQKMKEHIVSYTKEKGFFNFKSF"
     gene            23271..24467
                     /locus_tag="MJF80_001757"
     CDS             23271..24467
                     /locus_tag="MJF80_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBZ0280425.1"
                     /translation="MVKFAKRMENMKNSANIVRNLFNSMTDPEIISFGGGAPAIEALP
                     VDIVREISNEVLAKNKRGIEALQYSHPYGLKDLREAVAQKLLEPKGVHASPEDIMIVN
                     GGLETMNLICQVFIDPGDVILVESPTFVHCVEIFEMFQAKCIAVDMDEHGIIPEDIER
                     KIQKHHPKMVYVIPTFHNPTGRTLTTERRKILAGIGSKYDVLILEDDPYRDLRYSGEE
                     LPPIKAFDQTGHTILANSFSKIFSPGARLGYVLAEHAIIERLLDAKTATNSHTSAISQ
                     VLCAEFFNRGYFDEHLKNIRDIYRERRDVMIESIDKYLPKGTKRVFPDGGLFTWVELP
                     ENINTTELLKEAVEHKVAFVAGEGFFTEGGGKGRNCMRISFGNVPPEKIRIGMERLGQ
                     LIASKM"
     gene            24535..25281
                     /locus_tag="MJF80_001758"
     CDS             24535..25281
                     /locus_tag="MJF80_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBZ0280426.1"
                     /translation="MTNKVPNLWNQLENLKKSYKWVDLSREVSPETPHWTGFPAMEVE
                     PFYLLDRGDGFNVNKYTLVTQYGTHVDAPIHFVADRKYLDEYTPMDMVLPLCVIDKSD
                     EVAKNSDYVVTVEDIQAWEKEYGNIPEGAFVAFRSDWSKRPDDSLDNYDSDGNKHFPG
                     WSIEALQYMVEKRNIAAIGHETCDTDAPILSNVSGYQAETYILGENRYQVEFLVNLSE
                     LPAIGAIIFVTFPRIHKASGFTARCFAICP"
     gene            25314..25817
                     /locus_tag="MJF80_001759"
     CDS             25314..25817
                     /locus_tag="MJF80_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBZ0280427.1"
                     /translation="MSKKAMFVWCVGLGLLDGIYCILCSYLPHIQNYMWIGFISLPIY
                     FCGGAKLEEMPKYFCCAASGIFWGGLTLVVLDIGFISNIHLNMLVFVTIVVCIACFVH
                     LGVLTENVWKGLFSSCPMMFGGFAAIFSQGLAEWFWVLVTLTLGLIFGCIMASISTPI
                     TKWIDKN"
     gene            26065..26900
                     /gene="panB"
                     /locus_tag="MJF80_001760"
                     /pseudo
     CDS             26065..26900
                     /gene="panB"
                     /locus_tag="MJF80_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
     gene            complement(28418..28993)
                     /locus_tag="MJF80_001761"
     CDS             complement(28418..28993)
                     /locus_tag="MJF80_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBZ0280428.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
BASE COUNT        10490 a         4042 c         4295 g        10251 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ctttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa atacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttaacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcgt gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tgtattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata atttttgtat cattaattat
     8521 aaaaatagca tttttttcgt taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtatgaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctattg ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttactag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga cttttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagttat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 aggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga cattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaacttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggc ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac aaatagctta gttgagcttg gtcttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggtg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt acgatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gtttctaaat aaaaaggctg ctgtagataa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaaaggc gctggagatg atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ctagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgactgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttatt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaaaatgaag actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataagcc ttgtaatata
    18781 aattgattta tagacattat ttatacttaa aaaacaccta ttcaacattt aaaattaatt
    18841 taattcttaa aggggggaac atcatgggca aagttatagc tgtattcaat caaaagggtg
    18901 gagtcgggaa aactacaaca aatgtaaatt taagtgctag cttaggaact ttgggaaaga
    18961 aaatattagt attagattta gacccacaag gaaatacaac tagtggatat ggtattaata
    19021 aaaacgaagt agaaaataca atctatgaaa ttatgttaga tgggttacat attaaagaag
    19081 caataataag tactgaattt gagaatgtag atgtagttcc atcagctact gaactatcag
    19141 gtgcagaaat agagcttaca tctaaaacta atagagagta tattttaaaa aattccataa
    19201 aagcggtaat agatgaatat gattatatat ttttagattg tccaccctca ctggggatgc
    19261 ttacaataaa ttgtttaact gctgtagata gtgttttaat tccaatacaa tgtgaatatt
    19321 atgctttaga aggtgttagt caattaatgg agactatcaa attagtaaaa tcaagattaa
    19381 atgctgatat agaaattcaa ggtgttgttt taagtatgtt tgatggaaga gctaatttat
    19441 caatacaagt agttgaagaa gttaagaaat actttaaagg tagtgtatat acaactttaa
    19501 ttccaagaaa tgtaagactt gcagaagctc caagtcatgg aaaacctgta atttattatg
    19561 ataaaagatg tagagggtct gtagcttatt tggagctagc agaagaattt atagatttag
    19621 aagaggagga atggtagtat ggagaataag tctaaaagaa gtaatagatt aggtagaggt
    19681 cttagtgcat tgataccaga aattaaagga gaaacttcag aaaaagaaat agtcaacata
    19741 gatatagaca aaatatatcc aaacgaagtt caacctagaa agcaatttga tgaagaaaag
    19801 ataaaagtat tgtctgattc tataaaaaat tatggtgttt tacagccaat agtggttaaa
    19861 atggatgaaa ataataaata tatgataata gctggagaaa gaagatttag agcatcaaaa
    19921 cttgcaaata aaaatcaaat acctgctata attaaagata tagatatgaa agatataatg
    19981 gagatagcct taattgaaaa cttacagagg gaagatttaa attcaataga agaggctcta
    20041 gcatataaga gtttaataga gcattacaat gtaactcagg aagaaatatc agaagctgtt
    20101 gggaagagta gacctcatat aactaataca ctaagacttt taaatcttgg acaagatgtt
    20161 atagaaatga tagacagtgg tagaattaca gcaggccatg gtaaggcttt attgagaatt
    20221 gcagataaag atttgcaact tcaaatagcg aaaaaaatag aagaagaaga actttctgta
    20281 agagaagtcg aaaatatagc taaaaaaata tctgaaaata aacaagaaga acctaaaaaa
    20341 tctaaaccta aggatgtatt tattttagat gttgaagata aacttagaaa tatatttgga
    20401 acaaaggtga atatttctaa aggaaagaaa aaaggtaaaa tagagataga atactataat
    20461 gatgatgatt taaatagtat tgtatcaatg cttcttgaat agaacaaaaa tataaaacag
    20521 agaggaagat tgttatgatt tatttagata atgctgcaac tacatatcca aaaccagaaa
    20581 gagtgtataa tgctgtttta gattgtatga aaaattattg tgcaaatcca ggtagagcag
    20641 gccataaact tgctatgaga gctgctaggg agatatatga tacaagagag aatatagcta
    20701 agttgttcaa tgtaagtaat cctatgaata tagtatttac atctaatgca acagattctt
    20761 taaatttagc gataaaaggt gtattacaag aaggagacca tgtaataaca acgtctatgg
    20821 agcataattc agtaataaga cctataaaag cattggaaaa aaggggtatt gaaaatactg
    20881 tagtaaaatg cgattatgag ggatttttag attatgaaga tttagaaaaa tcaataaaga
    20941 ataatactaa attgattgta acaacacatg cgtctaatgt atgtggaact ttaatagata
    21001 taaaaaaagt tggagaaata gctaaaaaac ataatatact atttttagta gatgcatcac
    21061 aaacagcagg tgtttatgat atagacgtaa atgaatgtaa tatagatatg ttggcaatgc
    21121 caggtcataa atgcttattt ggacctcaag gaacaggtat attatatgta agagaagggt
    21181 tgaacttaaa tatattaaaa gaaggtggaa caggtagtaa atcagaagaa atagttcaac
    21241 cagagttatt cccagataaa tatgaatcag gaactcataa tactccaggt atagcaggtc
    21301 ttaaccaagg tatactattt atatttgaaa gaggaatcaa caatataaga caacatgaag
    21361 aagaattatg tcaatatatg atagacaaat tagaggaagt accagatata aagatatatg
    21421 gaccaaagga cagtaaaaaa agagcatcag taatagctct aaatatagga gatatggatt
    21481 ctggagaggt tactttctta ttagatagtg attataatat agcgactaga tcaggaatac
    21541 attgttcacc actagcacat accactttag gtactttaaa acaaggtgca gtaaggttta
    21601 gtataggata tttcaacaca aaagatgaga tagataaagc agtggaagct ttgaaaaaaa
    21661 tttcaaaaaa taaatagcat gagatttaat aatatagaaa taaaaaatgg ggatgtttca
    21721 aaataaaaat ttgaatagag tattgaaatt tttatgtata ttctttaaat aaaaacttca
    21781 atactctatt aattaaatta ctaattttgc taattttcat aaaatctgtt aaattactca
    21841 taaagttata tattttcaaa ggctcaagtc ttgtatgaaa ttacttgttt taatttttta
    21901 tataactatg acttttgcaa tctttttagt tgaaaagcgc cttcaatcat actaaaataa
    21961 taatattaca atgctaaatt attttatcct ctattataat tataaataca aaccggtgtg
    22021 actcatctta tgaaaaaagt attgttcagc gttgataaaa ctgacaatta agtaataaaa
    22081 atattatact aattttactt tttaaaaaga cttaaaatta aaaaaaccct tttcttttgt
    22141 ataacttaca atatgttctt tcattttttg aactgcaaaa gaatcatatt gtgagttggg
    22201 ataagctaaa ctaattacac ggtaatgctc tggtacaaat gggtatgtat caacattgcc
    22261 aacacaactt ttataaatta gttctgataa aatagcaatt ccaaaccctg cttctaccat
    22321 ggcaacaata gcactatcag tatctatgtg aaattgagag tacacattaa gatgatattc
    22381 acgaagaatt ttttctactt cctggtcata atctttttta ggcataacaa aggacatatt
    22441 ttggatatcc tctattgtaa catacccagg attacaaggt acaaatcctt ttggaaagac
    22501 acaaatgata cggtctttgt gaagcggaat agattcaata tgtggaggaa gtactgtgtt
    22561 ggatataaaa gctaaatcca caatagaagt ttcaatccat tctaggatat taatatagtt
    22621 cccttgaaga atatgtattt caatattagg atgttcctgc cgaaatgatt tcataatctc
    22681 agggatccag tttattgtaa tactattaaa agttccaata cgaataatac cactttctga
    22741 acgtgaaata cgattaattt cttgttctaa aatttcgtta ctatgtaata aaatacgaat
    22801 atgaggtaaa attttttcag cttctcttgt taaactaata ccatttctat ttctaataaa
    22861 caattgaaaa cctacatcta tttctaattt tgcaatcgca tgacttacag ctgacggagt
    22921 taagtttaaa atacttgctg cttttataaa actattttgc tctaaaactg tttcaaaaat
    22981 agtatatcca taaattgaca tatgatttac tcctttcctt tagatgagta aaaactcatc
    23041 taaaacgata aaaagattcg ctttatttta taataataat ctactattat taaattataa
    23101 atacaagtga tcatttgcat tttttactca agataataaa aatagtacca cccaaaatac
    23161 aagaattaaa atattatttc tgtttaaaag tgagcggaaa cggaaccttt gctcatctaa
    23221 tctcatataa ttacagacaa atatcaactt gataaaaagg aggaactaat atggtaaaat
    23281 ttgcaaaaag aatggaaaat atgaaaaatt cagcaaatat tgttcgtaat ctttttaatt
    23341 ccatgacaga tcctgaaatt atatcttttg gcggaggtgc accagcaata gaggctttgc
    23401 cagttgacat tgtaagagaa atttctaatg aagttttagc aaaaaacaaa agagggattg
    23461 aagccttaca gtacagtcac ccatatggtt taaaagattt acgtgaagca gttgctcaga
    23521 aattattaga accaaaggga gtacatgcaa gtccagagga cattatgatt gtaaatggtg
    23581 gtttggaaac aatgaatctg atttgtcagg tattcattga tccaggagat gtcattttag
    23641 tagaatcacc aacatttgta cattgtgttg aaatttttga aatgtttcag gcaaaatgca
    23701 tagcagtaga tatggatgaa catggaatca ttccagaaga tatagaaaga aagattcaaa
    23761 aacatcaccc aaaaatggtt tatgtaattc ctacttttca taatccaact ggcaggacat
    23821 tgactacaga acgaagaaaa atattggcag gtattggtag taagtatgat gttttgattt
    23881 tagaagatga cccttatcgt gatttgagat atagtggaga ggagttacct cctattaaag
    23941 catttgatca aacaggacat actattttag caaatagttt ttctaaaatc ttttctccag
    24001 gagcaagact tggttatgtt ttagctgaac atgcaatcat tgaacgatta cttgatgcaa
    24061 aaacagcaac taattctcat acatcagcta tttctcaagt attgtgtgca gagtttttca
    24121 atcgtggtta ctttgatgaa cacttgaaga atattcgaga tatttataga gaacgcagag
    24181 atgtgatgat tgaaagcatt gataagtatt taccaaaagg tactaaaagg gtatttccag
    24241 atggtggttt atttacttgg gtagagctac ctgaaaatat caatacaact gaattattga
    24301 aagaagcggt agaacataaa gtagcttttg tcgcaggaga aggattcttt acagaaggtg
    24361 gtggaaaagg gcgaaattgt atgagaatta gctttggcaa tgtacctcct gaaaaaatta
    24421 gaattggtat ggaaaggctt ggacaactaa tcgcttccaa aatgtaaata attacttata
    24481 taaaatacaa ctttatattt taagatttat aatataacca aaggaggaat acttatgaca
    24541 aataaagtac caaacttatg gaatcaattg gaaaacttaa aaaaatcata taaatgggtg
    24601 gatttgagtc gtgaagtaag tcctgagaca cctcattgga caggctttcc tgcaatggaa
    24661 gtagaacctt tctacttatt ggatagagga gatggattta atgtaaataa atatacacta
    24721 gtaactcaat atggaacaca tgtagatgca ccaattcatt ttgttgccga tagaaagtat
    24781 ttggatgaat atacgccaat ggatatggta cttccacttt gtgtcattga taaaagtgat
    24841 gaggttgcta aaaattcaga ttatgtagta actgttgaag atattcaagc atgggaaaaa
    24901 gaatatggta atattccaga gggtgcattt gtggcatttc gttcagattg gtcaaaaaga
    24961 cctgatgatt ctttagataa ttatgattca gatggtaata agcattttcc aggatggagt
    25021 atagaagctc tgcaatatat ggttgagaag agaaatattg ctgcaattgg acatgaaact
    25081 tgtgatacag atgcaccaat tttatcaaat gtaagtggat atcaggcaga gacatatatt
    25141 ttaggagaaa atcgatatca agttgaattt ttagtaaatt tgtctgagtt acctgcgata
    25201 ggagctatta tttttgtaac attcccaaga atacacaaag catctggttt tactgcccgt
    25261 tgttttgcaa tttgcccata aggttttaac tgttagaagg gagggggaga cagatgagta
    25321 aaaaagcgat gtttgtttgg tgtgttggct taggtctact agatggcatc tattgtattc
    25381 tttgttccta cttaccccat atacaaaact atatgtggat tggttttatt tctcttccaa
    25441 tttacttttg tggaggtgca aaattagaag agatgccaaa atatttttgt tgtgcagcaa
    25501 gtggtatatt ttggggtggg ctaacattag ttgtattaga cataggattt atatccaata
    25561 tacatttaaa catgcttgtg ttcgtaacaa ttgttgtatg cattgcttgt tttgtacatc
    25621 taggtgtatt gactgaaaat gtatggaaag gattgttctc aagttgccca atgatgtttg
    25681 gtggatttgc agcaattttt tctcaaggat tagcggagtg gttttgggta cttgtaacac
    25741 ttacactagg gttgattttt ggttgtatca tggcttcaat ttcaacacct atcacaaaat
    25801 ggattgataa gaattgaaat aaagtagcta ttcaataatt tttaaaaagt taatttattt
    25861 atgaaatata atggtatatt atttcaagat ggtttaaaag gaaaagtttt ttaatacttt
    25921 tccttttaat ttaaaatata aatacatttg ccacataagt gcttctttaa aatctagtat
    25981 tatctttact agatatagga gtaagttgtt ttaccaaatt agttttatat gaagatattt
    26041 acaaaatttt aggaggaaaa aacaatgaaa aatactatac aaacttttaa gaatgctaaa
    26101 agtgaaggaa aaaaactatc tatgcttact gcttacgatt actcaatggc aaaaataatg
    26161 gatgagtgta atataaatgg tcttttaata ggagattcat taggaatggt tgtaaaaggc
    26221 gaagaaaata ctctctctgt tactatagat gaaattatat atcatacaaa agctgtaaaa
    26281 aatggggcaa aaaatgcctt aatagtaagt gatatgcctt ttctatatct taccatgttt
    26341 ctatagaaga tgctgttaaa aatgctggta gacttgttaa agaaggagga gctcatgctg
    26401 taaaattgga gggtggagca aatgttataa agcaaataga aagcattgtg aatgctcaaa
    26461 tacctgtaat gggtcattta ggactaactc ctcaatctgt aaattcattt ggtggattta
    26521 aggttcaagg gattactagt gaagcagcaa aacaacttat tgaagatgct aaattaatag
    26581 aaaaagcggg ggcattttct attgttcttg aaggtgttcc tgcgaaaata tctgagataa
    26641 taactaactc tatttcaatt cctacaatag ggataggagc aggaattaat tgtgatggac
    26701 aaattttaat ttaccaagat atgttaggta tatttgaaga tttcgtacct aagtttgtga
    26761 agcaatatgc aaatgtagga gatataatga aagattcaat taaaaattac atactagaag
    26821 ttgaaacagg ggcatttcca caagaaaaac atagtttttc aataaaagaa tctgaattag
    26881 aaaaattata cgaagattaa ggatgttata agctgtcata tataacaatt tattattata
    26941 tttagagatg gattgtccat cttttctgga taaaaattta tccagtttta tgattataat
    27001 aaccaattgt aaaatatatc taaacaatat attatactat gtagattttt caatgaaata
    27061 acatcagata tttactgaat atttttattt cttatttttt agaggaactt cttttaattt
    27121 aaatataaaa ataatgaatg tgtttatata gtaactgcat atattaatag tttaaaagga
    27181 cactattcat tcgtagaaac ttaaattgat gattttatta aaaaatatgt agaagatatt
    27241 gtaggtttat gtcttcatag aataatgatt atattatatg aatgtgattt agcataaaat
    27301 tagatatatt aaagttgagt gagaagaatc ttgaacaaag ttcttcttac caactttaat
    27361 atatttctat tatacaatct attttttatc attattgaaa ttgtttacaa cttgatttaa
    27421 ctcaattaat atacttccaa cttccatata gagaatttta ctataaacac aagtaataca
    27481 ataggaatgt aattatcact tgattttttt attttttaaa tcccataaca aaacattaag
    27541 tgcaactatt ggagttaaca attgttgtag tataaaagct attaagttat ctatatatta
    27601 tcttcctaaa aaagttttct taacacttta attattattc aaaaactatt taaacaaagc
    27661 tataagagag tttaagctat cactccaata agatttattc tttattttat acatctaata
    27721 tttcaaattc gtaaatttac ttccaattat agaattatac ttgtttcgta tttattcaat
    27781 ttatcttata aaagcttttg ttttgtaaaa atattaattt tatattaaaa caagacttag
    27841 aaactatata cttcttgaaa aaattctaat atagataaat ttaaatatta taaatgaggt
    27901 atttttttaa atgaggaaga taatggtaga taattaaaaa tatattattt tatcatttga
    27961 tatacattta tttattcaaa aactataaaa gaacattaac ttttttagaa gtagaattta
    28021 caactaaaga ttgtaatatt gctattgaat ctaagcatta taaaaaacat tggatacaat
    28081 ttattggtat agcattttca aaatgtcaat atcacttgtt atattactgt aaattgtata
    28141 gattatctta gccttgaaag tatttgtgta tatatggtga ctatgataat tgacaatcta
    28201 gtaaataaga taaagataac attagtagga taaaatattg gtatataaaa gatctaaata
    28261 gaacctgaaa ggtgttgttt caaaattgat tttttataat caaattttta tttctatttt
    28321 tatgtttttg tatgtaaaaa agaaaggaag gagataattc aaatttttat tttgaaccat
    28381 ctccttctat atgataattt ataattgttt caagaaatta ctttaagtaa tctccttctt
    28441 caagaactat atgaccactt tcgtaattag aaccatcaac agtaattttt atttgttcaa
    28501 cattcatatt ttttatgtaa gtacgagcaa ctgaatcaag cattaaagtt tctgcatcac
    28561 tacctaagtt tgactttgta aagtcagagc taacatctaa tataccagtt tttacaccat
    28621 caatgtcttt tgtagtaaat gaatttaatt ttgcagattc tggaacaact ttaagttttt
    28681 gaagttcttc aaataaagtg ttctcatcaa ttttatttaa atcaacttta ttttcaatta
    28741 aatctgtctt atctacatca aaagagtaaa tagtagctga ttgagtttta gtttcttctt
    28801 ttggagtagg tttagctttg ttattaggaa cctctttttc agtattgttt tcttcttttg
    28861 gttgctcaac atttgtattg ctcttagttt cttttttaga tgaatcactt ccattttgag
    28921 cattagtaca accaactgcg aatatagata tagataatat acttaacact aacattattt
    28981 tcttattttt cataaaaatt tctcctctct taaaaactat tcttatatat aattatagtc
    29041 gttatgagca aatatatgaa tacaaaatag taacaaat
//
LOCUS       DAGSWI010000045        29026 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.045, whole genome shotgun sequence.
ACCESSION   DAGSWI010000045 DAGSWI010000000
VERSION     DAGSWI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.045"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            135..1124
                     /locus_tag="MJF80_001762"
     CDS             135..1124
                     /locus_tag="MJF80_001762"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBZ0280429.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            1196..1936
                     /locus_tag="MJF80_001763"
     CDS             1196..1936
                     /locus_tag="MJF80_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBZ0280430.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            2152..3303
                     /locus_tag="MJF80_001764"
     CDS             2152..3303
                     /locus_tag="MJF80_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBZ0280431.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            3328..4083
                     /locus_tag="MJF80_001765"
     CDS             3328..4083
                     /locus_tag="MJF80_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBZ0280432.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            4275..6662
                     /locus_tag="MJF80_001766"
     CDS             4275..6662
                     /locus_tag="MJF80_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBZ0280433.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVDDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(6893..7126)
                     /locus_tag="MJF80_001767"
     CDS             complement(6893..7126)
                     /locus_tag="MJF80_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBZ0280434.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            7415..8005
                     /locus_tag="MJF80_001768"
     CDS             7415..8005
                     /locus_tag="MJF80_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0280435.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            8119..8964
                     /locus_tag="MJF80_001769"
     CDS             8119..8964
                     /locus_tag="MJF80_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280436.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            8961..9644
                     /locus_tag="MJF80_001770"
     CDS             8961..9644
                     /locus_tag="MJF80_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280437.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGNKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            9641..10357
                     /locus_tag="MJF80_001771"
     CDS             9641..10357
                     /locus_tag="MJF80_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280438.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFVITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYIYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            10454..10717
                     /locus_tag="MJF80_001772"
     CDS             10454..10717
                     /locus_tag="MJF80_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0280439.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            10689..11144
                     /gene="def"
                     /locus_tag="MJF80_001773"
     CDS             10689..11144
                     /gene="def"
                     /locus_tag="MJF80_001773"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBZ0280440.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            11196..11657
                     /locus_tag="MJF80_001774"
     CDS             11196..11657
                     /locus_tag="MJF80_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBZ0280441.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHVLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            11707..12609
                     /locus_tag="MJF80_001775"
     CDS             11707..12609
                     /locus_tag="MJF80_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBZ0280442.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            12979..13791
                     /locus_tag="MJF80_001776"
     CDS             12979..13791
                     /locus_tag="MJF80_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBZ0280443.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            14839..15600
                     /locus_tag="MJF80_001777"
     CDS             14839..15600
                     /locus_tag="MJF80_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBZ0280444.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            15665..16447
                     /locus_tag="MJF80_001778"
     CDS             15665..16447
                     /locus_tag="MJF80_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBZ0280445.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKCKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKKIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            16714..17133
                     /locus_tag="MJF80_001779"
     CDS             16714..17133
                     /locus_tag="MJF80_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBZ0280446.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNKKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(17236..17502)
                     /locus_tag="MJF80_001780"
     CDS             complement(17236..17502)
                     /locus_tag="MJF80_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBZ0280447.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            17729..18430
                     /locus_tag="MJF80_001781"
     CDS             17729..18430
                     /locus_tag="MJF80_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280448.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            18512..19120
                     /locus_tag="MJF80_001782"
     CDS             18512..19120
                     /locus_tag="MJF80_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBZ0280449.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            19444..19908
                     /locus_tag="MJF80_001783"
     CDS             19444..19908
                     /locus_tag="MJF80_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HBZ0280450.1"
                     /translation="MKKLVIILISIISVVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNIWH
                     GSVDNDTFAISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            20545..21561
                     /gene="gap"
                     /locus_tag="MJF80_001784"
     CDS             20545..21561
                     /gene="gap"
                     /locus_tag="MJF80_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBZ0280451.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            21956..23125
                     /locus_tag="MJF80_001785"
     CDS             21956..23125
                     /locus_tag="MJF80_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280452.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            23505..27758
                     /locus_tag="MJF80_001786"
     CDS             23505..27758
                     /locus_tag="MJF80_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBZ0280453.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            27907..28401
                     /locus_tag="MJF80_001787"
     CDS             27907..28401
                     /locus_tag="MJF80_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBZ0280454.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            28517..28894
                     /locus_tag="MJF80_001788"
     CDS             28517..28894
                     /locus_tag="MJF80_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBZ0280455.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT        11726 a         2846 c         5039 g         9415 t
ORIGIN      
        1 gtcataaatt aaattaaaat attttaatga gatatgactt ttttagaatt aaaaaccgta
       61 gtatattgat ataatagaaa taagacttaa aaatctgttg atagtattat ttataagtac
      121 agtaggagga taaaatgttt gaaaatttac ctaagataga tttacattgc cacttagatg
      181 gaagtgttag agtagaaacc atgcttgata tagctattaa agagaaaata gatttacctt
      241 caaataatat ggatgaaata aaaaaattag caaaggtttc ttttaattgt acttcactag
      301 atgaatattt agaaaaattt gatttaccct taaaagtaat gcaatctaag gaaaatttaa
      361 aaagaattac ttttgaactt ttggaagatg caagtagaga aaatgtaaaa tatattgaaa
      421 ttagatttgc accattactt cacacccaaa aaggtatgag tgttaaaaat ataattgagg
      481 gtataattga aggaattaga gaagctgaaa gtatttatga tataaaaggc aatttaatat
      541 taggttgtat gagaacaatg acctcaaaag aggcattatt agtaattgaa gaagggaaat
      601 catttgtaaa taaaggtgtt gtagcagtag acttgtgtgg tccagagaaa gagggatttt
      661 gtaaggaata taaagatgtt tttaaattag caagagaata tggatataaa gttacaatac
      721 atgcaggaga agctgcaagt ggagaaaatg tattggatgc tataaatatt ttaaaggcgg
      781 ataggattgg tcatggggta aaaattaaag accataaaaa ggcgtataat ttagttaaag
      841 ataaaaaaat attgcttgaa ttatgtccaa ctagtaatgt acaaactaag acagtagatt
      901 cttatgaagc acatccattt tatacttttt ataaagataa tttacatgta agtataaata
      961 ctgataatag aactgtatca gatataaatt taagttcaga actaaatgtt atatttgata
     1021 cttttaaact aggattagaa gactataaaa taatttatag aaatgcagta gaagctagtt
     1081 ttgcagataa agaaactaaa gagtatttga atagtttaat ttaatagata aaaatattta
     1141 aactttaaat taagctatta aattgataaa aatacattgt attaggagaa aaaatgtgaa
     1201 agattatgaa gatgttgttt atattaaact agtcaaaaaa gaacttgaag aatggaagaa
     1261 aaatatatta aagaaacctt ctatatttga caaagcatca aagggtatac agaataaatt
     1321 taatggagta ttgccagata aatatcatga aataattact tcttctataa aagtaatgac
     1381 aaaaaccgta ctgtttggat ataaatatgc tacaaagcca ccacttagaa atatgtcatt
     1441 agaagaaaga gaaaaattag ttttagaaaa agctaaaact tacaagacta cagcaatgat
     1501 tgaaggggca ggaacagggg caggaggaat cctcttaggt ttatctgatt ttcctttgct
     1561 tttaggtatt aaagtgaaat ttttatatga tgtagcttca atatatggat atgatgttaa
     1621 agattataga gaaaggattt atatattaaa tatattacag ttggcttttt ctagtcaaca
     1681 gcatataaac aaaactttta aattgatgaa taactgggat gaatatgtaa atagtctacc
     1741 agaagacatc aatgattttg attggagaac atttcaacaa gagtataggg attatttaga
     1801 tttagctaag tttttacaac taatgcctgg aataggagca tttgtaggat tttatgtgaa
     1861 tggaaaatta attgataaat tatgtgaaac tgctgtgtct gcttatagat tgaggattcg
     1921 agagcttaag ttataagaaa aattataaca taaaaaatta gttatacaat atatctatat
     1981 gtatctaatt ttgcattgat aaaaacaatt atggataaaa ggtaaagcat gatagaatct
     2041 aatgtagagg tttgtatttt aatggaaaaa tttagttttt attgcttaag atacaaattg
     2101 aattacgaag agattgaatt atgaagagaa ataattggga ggaatatttt catgacaaat
     2161 ttgccaaaac aatttgatag cttcaatgaa gctagacaaa aaggatttat aaatgcaaag
     2221 gaacttaagg aaagtgggaa aaagatggtt ggagttttct gtacatttac tccagttgaa
     2281 attccaatgg cagctggagc aactgtagta ggtgtatgcg gcgttagtga agagccaata
     2341 cctgatgcag aaagggtgct tccaagaaat ctttgtcctt taattaaatc aagctatgga
     2401 catgcaataa cagatacctg tccttatttc tatttttctg atttgttgat aggagaaacc
     2461 acttgtgatg gtaagaaaaa aatgtatgaa gaacttgcaa aagttaagcc aacatatgta
     2521 atgcatttgc caaatacttc aaaaggagaa tttgcctata aactttggaa agatgaaatg
     2581 ataagattaa aagaagaggt tgagaaatct ttaggtgtaa ctataactga agaagatata
     2641 agaactgcta taaaagataa aaatgaagaa agagaacttt taaaagagtt ttatgcactt
     2701 ggtaaattac aaccatctgc acttacaggc cttgagttac ataatgtttt atatcaagca
     2761 ggttttaaat ttgaccgtgc agaacttaaa cattctttaa gaaaagttat agatgatatg
     2821 aaagaaagat atgaaaaagg tgaatgtcct gttcaaaaag ataagccaag aatattgata
     2881 acaggttctc ctattggtgg aataagcgaa aaaatagtta aaactttaga agatgcaggg
     2941 gcatcagtcg ttgcatatga actttgtgga gctattagat caaacgattt attggttgat
     3001 gaagaaatag aagatgtata tgatgcttta acacaaaaat atataaatat aggttgttct
     3061 tgtatgatga ataatgacaa tcgtattgaa ttattagata gaataataga tgaatataat
     3121 gtagatgctg taatagatgt agttttacaa gcatgtcata catttaatat agaaagttat
     3181 agaataagag aatttgttac aaaagaaaaa aataagccat ttatgtctct tgaaactgat
     3241 tattcaaaat cagatactga acaattaaga actagatttg aagcgtttgt agagatgttg
     3301 taggaataaa taaaagtagg aggaactatg tttagtatag gagttgactc agggtcagtg
     3361 gcaacaaagg gtgtgctatt tgatggtgaa aaaattatta agaaaataat tataccaact
     3421 ggatggagcc ctaaaagtac atctaaacag gtctatgagt tgctaagtag tgaaattgac
     3481 aaaaaagata taaaaaaggt ggtaggtaca ggttatggaa ggggagtaat ggattttgct
     3541 gataagaaag taacagaaat aacttgtcat actagaggta tttacttttt aaacaagaac
     3601 ataaggacta ttttagatgt aggtggtcaa gatagtaaag ttataaattt agatagagat
     3661 ggaaatgtat ttaactttat aatgaatgat aagtgtgctg caggaactgg tcgattttta
     3721 gagattactt ctaacctttt aggaagcgat attgaaagta tagatacatt agctaaagga
     3781 tatgaaccag ttaatatatc tagtatgtgt acagtttttg ctgagtctga aattgtaagt
     3841 cttctagctc aaaacatttc tactggagag gtagcagcag ggatacttaa atctattgca
     3901 aataaatcca cctcaatgtt agcaagaggt gaggtaatag atgaagttgc atttactgga
     3961 gggcttgcaa aaagtaaaga actagtaaaa atgatagaag aaatactggg taaaaaaatt
     4021 tttattgcag aagatactca aataatagga gctttaggag ctgctgtaat aggatttaga
     4081 taaaaacata taaaaagaaa atgagaagct gaatttatta taaataaatt cagtttttta
     4141 ttttatttta tttatttata aaggaaaact ttggtataat tgatttaaat gattgtatat
     4201 ttgtaaatac ttgatattta gtcatatata acattataat tttaaaaatt agctcaaaat
     4261 aagggggaga aagcgtgaaa aaatttactt caaaaaaagt aacaaggttt gttgcgctgt
     4321 ttttaatatc tattttagta atgtcaacaa ttcctgtatc agctgacaat agcaatacat
     4381 tgaatcagat aaaaaaagca gaatacagtg agacgtataa acaatatcta aatgatgcta
     4441 aaaatggacg tacagaaaaa tataatggga ttataccaaa tccatatcag ttggaaggta
     4501 ctcaaatcca aagtaaagga agaacagctc caacatctta tgaccctaga aaattaggtc
     4561 ttatgacatc aataaaaaat caagaggatt taggaatctg ttgggatttt gctgcaatgg
     4621 caaccttaga aagtttctta aagttaaata attatggaga ctatgattta tcagaagaac
     4681 acttaagatg gtgggcttca gatggtgaat atggatggtc tgtagatgat atgaatggtg
     4741 ctttaaatta tgaagctatg ggatatttaa catcttggtc aggtcctaaa ttagaaaaag
     4801 atatcccata caatggtaga gtatctaaag cacaaggagc aaaaaaacca actaatatga
     4861 atacagcacc tactgttttt aatgtgacag atgctgtgtg tgtaagtaat gacataaatt
     4921 ctacaaaaaa tgctatatta cagtatggag cagtaacatc tggttattat gaagatatta
     4981 aatatcaaag tgatgaccaa aattcatatt attgtccaac aaaaagtttt aatacaaatc
     5041 atgctatttc aattgtaggt tgggatgata actattctaa agataatttt aatgcaaata
     5101 ttagaccatc aaaaaatggt gcttggttaa taaaaaatag ctggggagat tataattctg
     5161 agggtggata tttctggata tcttatgaag acaagacact tatgtctgat atagataatt
     5221 tttcaatcaa aggtggtaaa aaaccaaatg atgatgagaa gatgtatcaa catgactatg
     5281 caagtattgt acctttaatt tcaaaaaaga taacagctgc aaatgtattt gatttcaata
     5341 gaggcgatga aactctaaaa tctgtaatgt tcttaactga aagtatagga gctaagtatg
     5401 aagtatatta tgctccagta gtaaatggta tacctcaaga aaacaatatg aaaaaattaa
     5461 aagaaggtac tgcccaatat tcaggataca taacagttcc aattgattct tttgatatac
     5521 cagaaggaaa gggtgcaatt gtagtcagca tagaggggaa aaatggagag tcaactatag
     5581 gttctgagtc aaatgtacca ggatatgata catttaaagc aaaagctaat ttaggtgaaa
     5641 gttatatcat agataatact ggaaaattct ttgatataaa tagagatagt aacttctatc
     5701 cgtgtaattt tactataaaa gctgttacag aaaagtcttc tggtgagagt atcccaaatg
     5761 agtctttaat tggtagtgat agatatgaaa ctgctatcaa agttagtcaa aatggtttca
     5821 attcttcaga aaatgtagtt ttagtaaatg gcagttcaat agtagatgca ttggcagcaa
     5881 ctccttttac atctgctata aattcaccaa tattattgac acaaaaagaa gctttaaatt
     5941 caaaaactaa agctgagata cagagattag gtgctaagaa agtataccta ataggcggag
     6001 aaaattctat aagtaaagaa attgaacaac aattaaaaag cttaaatata tcgatagaga
     6061 gaatatcagg aagtgataga tacaagacaa gtttattatt agctcaaaag ttaaatggta
     6121 taaaaaatgt atctcaaata gcagttgtaa atggagtgaa aggattagct gatgcaatta
     6181 gtgtaggcgc tgcagcagca gaaaataaca taccaatcat acttgcaaat gaaaagagcg
     6241 aattgcaagg agctgatgaa tttctaaatt cattaaatat agagaaatct tatattattg
     6301 gaggaacagc atccctatca aacaatctag aaagtaagtt aaaaaatcca actagattat
     6361 ctggtagtag tagagacgaa actaattcaa agataataga taatttttat aaaaaagata
     6421 ctttaaaaaa tgcatttgta gttaaaaatg gaatcaagaa tcaaaatgat ttaatagatg
     6481 gattggcagt tggtccttta ggagcaaaaa ctgggtcacc agtaatttta gttggtgata
     6541 aattagctga tagccaaaaa gaagttctta aaaataagac tttagaaaaa gtaactcaag
     6601 ttggtggagg agcaaataaa aatgcattta aagagttagt aaaattaaaa agtgctaaat
     6661 agagtactta agtattaaca tatattagtg tgtattagta acttatctta ataaaagctg
     6721 aaaataatta cttcaaatta ttgataaata agataatcaa tgattgtaaa gttttgtttt
     6781 cagctttaaa tttatgtagt ataagtttaa aaacataaaa aagggttact ttatatagaa
     6841 atatagaaaa tcttacattt tatatataaa taacccatat ttaattaact ttttatagtg
     6901 ttctaataga tattattgaa cttaatggta aatataatgt gtaagtttca tttaaagttg
     6961 gattagattc atctatatat cttgagcaaa gtgtaattga taagctatct gcttctataa
     7021 tttcaccata tagaggacaa ctgctaaagt aagaatgtac ttcaacatat ttatttaagt
     7081 tttcttgaac ataatcgtaa aaacggtttt ttgtagtagt agccataaaa tcaactcctt
     7141 tattttttat taaagttgaa acttttaaaa agataaatat gattacttct attagaaact
     7201 aagttaaaaa ataagcaaat agatatgaaa attagaataa tatattattt atatcttaat
     7261 tataacacta gataataaaa aatgctatat aaaattcaga attttcgaaa gtttttaagc
     7321 ttatttttaa atatactaat aatactattg acattagata gaaaaagtat tatattgact
     7381 atataaatta ttttagtcaa actggaggga aaagatgcct aaatcatatt ctgataaaga
     7441 aagagagtat ataataaaaa gacttaaaga agaagcaaga ttgtgtatgg agcaatatgg
     7501 tattagaaaa actactgttg atgagcttgt aaaacgggta aaaataccaa aaggaacttt
     7561 ttatctattt tttcaatcaa aagaattgct cttttttgag gtattaagag atatacatga
     7621 ctctatacaa aaagaaatct tatatgagat aaatagagta gatgaaagta ttacatgtga
     7681 acaattgaca gatatattta tgaaattcta taggatggtt gatagtactt caattttaaa
     7741 tctcatgata aatggagaat ttgagatatt ggtgagaaag ttaccagatt ctataattga
     7801 agaacacttt agacatgatg actttgaaat tggagagatt atatcttgca taccaaatgc
     7861 aaaaaataaa gacatagaaa gctttagtgg agcatttaga gcagtttttc ttaccatgct
     7921 atataagcgc gaggttggaa gtaattgttt tgaagatgct ttaagattaa tgataaacgg
     7981 attagttata caacttatgg aatagaattt aaattttgtt tgctaataac ttttattaaa
     8041 aattattaac aaacaaaaaa attatatttt attgaccata tagtcaaaat agtcaaaaat
     8101 ttgaaagggt gatattgaat gattaaagtt gatgatttat catttagtta tacagataga
     8161 gattttttac aaaatattaa ctttgaagta ggaaaagggg aaatactagg atttttagga
     8221 ccatcaggtg caggaaaatc tacattacaa aagattctaa ttggaatgat aactaattat
     8281 ggaggaagtg tcattgtaaa tggagtagag agtaaaagac actcaaataa attttatgaa
     8341 aatataggag ttgattttga atttcctagt ttatatgaaa agttgactgc aatagaaaat
     8401 ttaaaatatt ttgggtctct atattctaaa aaactactat ctattgatga gttattaaaa
     8461 tcagtaggac tagaaaatga agcaaataaa agggtatctg agtattcaaa aggtatgaaa
     8521 tctcgattaa attttataaa agctttatta cataatccag atattttatt tttagatgaa
     8581 ccaacaagtg gtcttgaccc gtcaaatagc aaggttatga aagatataat tttatcagaa
     8641 aaatctaaag gtaaaactat tattttaaca actcataaca tgttagatgc aacagagctt
     8701 tgtgacagag tagcgttcat tgtaaatggt aaaatatctg ctttagatac accacataat
     8761 cttattatgt caaaaggagc tattaaagtt agatatactt actttgataa tggagaaaaa
     8821 acttctgaat gttttctgaa caatactgct aatgataaaa atttgaatat gcttattgag
     8881 aaaaataaac ttttatcaat acatagtagt gaaccaacat taaatgatat ttttattgag
     8941 ataacaggga ggaatttaca atgaggctaa aaaatcttat tttaggagat ataaagtttc
     9001 aatttaagta tggtttttat tttttatatc tttttttaag tattatatat atttgtatta
     9061 tcaatgtatt tccaactttt atgagagaaa aaatagctat aattatgatt tattctgacc
     9121 cagcagcaat gggattgttt tttatgggag ctattgtact tcttgagaaa agtcaaaggg
     9181 ttttaaattc cttagctgta tctccagtaa aagtatctga gtatatttta tcaaaggtaa
     9241 tttcattagg agtaatatct agtattgtag ctatgtttat tgctattaca ttaaatttag
     9301 ataatattat aatatcaact ataggtacct ttttttcttc tattatattt tctttgcttg
     9361 gattgataat tgcaagtaaa gcatctagtt tgaatcagtt tattgtttta agtattccta
     9421 ttgagattat atgctttatt ccaccaatac tgaatgtact tcttgacaca aaatcatatg
     9481 caaatttgta cccttttaat atatgtattt ctcttattag tggaaataaa aattttataa
     9541 tgattaatat tttaatttta atatcaatta taataataat ttattttata acttattatt
     9601 ttatttgtag cagttggaaa aaggtaggtg gagtaaaact atgataaaga ttataaatgc
     9661 gtttaaacaa gagtttgaac aaattaagag agatgctatg ctttttatag tatgtgtttc
     9721 tccaatatta tgtggagtat ttataaaatt tggaatacca ttaatacaga atatatcact
     9781 taacaagttt tattatcaat taaatttaga gccgtatttc ttgatgtttg atttactttt
     9841 agcttttatt acacctttta tgttcttttt tgcttctaca atggtcatat tgggagaaat
     9901 tgatgacagc atttcaagat atttaatagt aactccgctt ggtaaaactg gttatcttat
     9961 ttcacgattt ggaataccag gtatcttagc atttgttatt actatgattc tacttatctt
    10021 tttttctcta actaaaattt ctttcttgtt aaacttatca atttctttat tagttttatt
    10081 acaaggaatt attatatcaa tattagtgat ttctttatca tcaaataaac tagaaggaat
    10141 ggtaattacg aaattctcag gtgtttttat gatgggtatt ttagcaccat tttttatttt
    10201 aaataaagca caatatattc tgttcttctt accaactttt tggatatcga aggcatttaa
    10261 ggaagataat tatatctata tgtttatttc aattgttata tcattaatat ggattttatt
    10321 attatttaaa aagtttagta aaaaaatagt taaataagaa aatttaatat ataaatgtaa
    10381 ggttaacttg acagtaatgt tgaaaaatgt tacatttaaa atgtaaagtt aaccttacat
    10441 gaatggagca aatatgaaaa ataaaataaa gatattaaga gaaaaattag gattgacaca
    10501 agaacaacta ggaagattag ttggaacatc tagacaggct ataaatgcta tagaaactgg
    10561 taaaaatgaa ccttcaatat ggttggcata tgatatttca agaattttta atgaaccaat
    10621 tgaaagtgta tttttatttg aagaaagtga aagaaaatca agagcacaga taagtagggg
    10681 agaatattat ggctataaga aagataagaa catttgatga tgaaatttta agaaaaaaga
    10741 gtaagtatgt agaaaacgta gataataaga taagagaaat attaaatgac atggcagaga
    10801 ccatgtacaa tactccaaat ggaggaggat tagctgcatg ccaagtagga gtattaaagc
    10861 gattagttgt tatagattta ggagaaggac ttattaagct tgttaatcct gaaattatta
    10921 aacaagaagg ggaacaaatt gttgttgaag gatgtttaag ttttcctgaa gtatggggta
    10981 aattaaaaag accaaaaaaa gttacagtgc aagctctaaa cgaatacggt gagaaaatag
    11041 aaattaaagg ttcaggtttt atggcaaaat gtctttgtca tgagatagac catttaaatg
    11101 gtatagtttt tacagataaa attattgaac atgtaaaatt gtaagataga ttggttattg
    11161 aatttataaa atattttata gtaaggagtt aaattatgag attatggcat aaggatttaa
    11221 ttgatgtatt acctaaaaat caattagtaa gtcaatggag agagctactt gctataaaag
    11281 gttctataga taaaaaaggt actccaaacc atgtattagt gaataaagta ttgaattata
    11341 gtatagatga atttaaattt tatacaaaaa ttgtacatga tgaaatgtta aaaagaaatt
    11401 ataaacctaa tgaacttaaa tatacaagta tattaaaatg gaaaaataga aattttgcaa
    11461 atgatatatc taatgaacat agtttaaatt tagaaaattt atatgatgat tggcataata
    11521 aaatgtattt aaagcaatgc ttatataatt tagaagaaaa agcaacatgt ggaggaatac
    11581 caataaatga atggaatata ttactttgta aatatagtaa agattatgaa ttgtggtctg
    11641 gaaatatcat gttttaatta ctatagatta acattatagt attattgtat ataaagaggt
    11701 gattatttgt ataataaaat aaaatatatt ccaataaatt gtgaaatagc ttgtaataaa
    11761 ttaaaaagga gtatgccata taaatgggat ttaaatattt atcgtggatg tgagcatgga
    11821 tgtaaatatt gttatgctat ttactctcat aagtacatca actccaataa ttattttgaa
    11881 gagatttatg taaaaacaaa tatagtagaa aaacttgaga gacaacttaa aagcagtaaa
    11941 tggaagaaag aagtaatcaa tataggagga gttacagata gttatcaacc aatagaagct
    12001 gactataaaa ttatgccaga aatattaaac ctgctaataa aatacaagac accagctatt
    12061 atttctacaa agtcagattt aattttaaga gattacgatt taattgataa gctatctagg
    12121 attacatata tcaatgtagc atctacaatt accacagtaa atgaagatac acaaaggctt
    12181 attgagccaa atggtgtgga ttcaatgaga cgatttaaaa tgttaaaaga atttagaaaa
    12241 actaacgcat cagttggtct acatattatg ccaattatac catatattac agatgatttt
    12301 gataatataa attcgctatt tagacatgca aaagaatgta atgtacatta tgttttacct
    12361 gggactttat acttaagggg tataactaga ggtgtttttt ttgactttgt taaaaaagag
    12421 tttcctgaat tatttgataa attatcaata ctatatagtg caggttcact taatagggag
    12481 tataaaaatc aattgtataa aatggttaat gaacttagat ataagtattc tctatcaagc
    12541 agttactcta aaataatgaa ggaaaagtta aaaaactcag gagatgtaca gatatctttt
    12601 tttgattagt tttatataaa aaaatttttt ttactatgat gattaaaatt tattaaaatt
    12661 aaaatttatt gacaaatata aaatttaaat atataattaa ttcataaaat aaaaatatga
    12721 aaggatgatg ttgttcggtg atatttgata tcataaaatc gttgatataa gtattgctgg
    12781 aaaagagtga ataaaatcaa atttaataag caaacaaagt aaagctatag ttttattgtt
    12841 gtgcttaaaa atatttggtt ctattttact cttatttaaa ctcatctttt tgaagttaaa
    12901 ccacagagta aactctgtgg tttttttatt ttaaaaataa attttaagga gtggtatgtt
    12961 ttgttaaaca aattatgtat gtatttagaa agaccagagc tttataaaca aagtgaaatt
    13021 aatttttggg atgatgagta tatttcaaag caattattaa aagcacactt agacactaat
    13081 tttgaaggag caagtagaaa ttttaacttt atcgaggatt ctgtaaattg gatagttaca
    13141 gttgcaaatc cagctaatta tcctaaatta cttgatttag gttgtggtcc aggtttatat
    13201 gcagagaaat ttgcacaaaa aggttataag gtaactggaa ttgatttctc aaaacgttct
    13261 attaactatg cacaaaatag aaataaagag actaatttaa acataaatta cttgtttcaa
    13321 agttatttaa atatgaacta taatgaggaa tttgatttgg ctactttaat ttattgtgat
    13381 tatggagctt tgtcaactga aaatagaagg ttattaatgg aaaaaattta tgatagttta
    13441 aaacctggag gaaaattaat attagatgta tttacaataa ataaatacaa taattttgag
    13501 gaaattaaat attgggaaat aaatgaagat ggaggatttt ggagtaatga aaaatatatg
    13561 tgtctacaag ataattgtaa atacaatgat tataatacat tagaacaaac tcttgtaata
    13621 accgaaaaag atgaaaatgt attttatgtt tggaatcact atttttcaaa agaaagtttc
    13681 ttattagaag ttgaaaatat tggatttaag tctgttgagt tttttagtaa tgttaagggt
    13741 gaagcatatt ctgacgatag tatgactatg ggattagttt tacaaaaata ataatttttt
    13801 taaaatgaat aaataaaaac ttgaaacctt taacaataaa ataacataat aaaagcttac
    13861 atattgaaaa atggaaactt ttatgttatt tttagtacat tacaaatttg cttatataaa
    13921 aatcaatact tgaattatta tttttatgtg aaatttatct atagtatatt tttataaaca
    13981 agtaatgtta ttaaaattaa gtatatagtt aaaagtgtat agcaaaaagg ctgttatatt
    14041 aatgttagag gttcagggaa aatatataaa gcaaagtatt tacagataat gtagattgca
    14101 cagctataag ttagataata gaattatgta atcaagaatt tacaacaaag tataagataa
    14161 ggataatgct aaattgccat acaggtgcta gatgtgtaaa tggatttaat taatactatt
    14221 gataaagtgc aacaagcaca taaaccaatt gatgagatta ttgaaactat aagagatata
    14281 gttgacataa ttaaaatatt ggaacctgtt tataatttta aatcaaatca ataatgatta
    14341 agattaaaat aatattaagg catcaatgtt ataagagtac aatataagga gtactatata
    14401 gtactcctta cttatacata ttaggcaata ggaaattttt tcatagttaa attttcaagt
    14461 attttttagg aaactttata attgagaatt aagggtatag tatgtgatga acttactgaa
    14521 tttataatta ggttatataa tggaataatc atcaatagtt ttactttagg gaaaatatat
    14581 gcttaaggaa gtatagctta ctaatattat taaaatgtat atttaaaatg ataaaaaaag
    14641 aattagacaa gtatttagta aattgactat gcaaaattct atcaagcagg aaccctatga
    14701 ctttagtcat gggaggttca gtaggtaact tgttgaatta agtttatatt atttaaataa
    14761 actacttaat ttagataata taaaaagtca gaattttaag aatagagcta tataatctta
    14821 gctattagga gaattactat gataggaata tattttagtg gaacaggaaa ttcaagatac
    14881 tgtgtagaaa aatttttaca agaatatgat ataacagcaa attcttattc tattgaagat
    14941 aatgaacttt tacagcacat caataatcat gagaatatta tttttagtta tccggtgcaa
    15001 tatagtaatg tgccaaaaat attgaaagat tttataataa atcattcaac tttgtggaat
    15061 ggtaaacgtg tttttattat tgcaactatg gctctattta gtggagatgg ctctggtgtg
    15121 ttatctagaa tattaaaaaa atacggtgct ataactgttg gtggattaca tgtgaagatg
    15181 ccagatagta taggagatga aaaggtatta aaacattctt tagagtataa tagaaagctt
    15241 gtaataaaag cagaaaagaa agtaactaaa gctgctaaaa aactgaaaca tgggtcacca
    15301 ccaaaagaag ggttaggttt tttgagtcat attgtagggt tatttggaca acgattgtat
    15361 tttatcaaca aaacaaatat gtatacagat aaactaaaaa ttaatactga ggaatgtatt
    15421 gtatgtggta aatgtgttaa tttatgtcca atgaagaatc tagtaattaa aaattgtatg
    15481 attgttgcaa atgggcaatg tacaatgtgt tatcgttgtg taaataattg tgctaaaaaa
    15541 gcaattacat tgttaggtaa aaaagttgtt gaacagagta atattaaaaa atatatgtaa
    15601 gaaatttaaa gttttattga atgtaaattg atgtatattt tatgttagaa agagaggtta
    15661 aataatggaa tactacatag ttgattcatt tgcaactaaa ctatttaaag gaaatcctgc
    15721 tggagtttgt gttcttgaca gaagaatacc tttagagtta atgcagaaaa tagcagaaga
    15781 aaacaattta ccagaaacag catttgtagt taaatgtaaa ggaaattatg aattacgatg
    15841 gtttactcct aaagcagaaa ttgatttatg tggtcatgca actttagcag cagcttatgt
    15901 aatatcaaat tttattgatg ttaatgtaaa aaagatagat ttctttactc aaagtggaaa
    15961 gcttgaagtt acacgaaatg gaaatctata tgaaatgata tttccagaaa taatgcctat
    16021 tgaaatagag ttatcaccgc aacaagctaa tttgataggc tgtgtaccaa gtgctgttta
    16081 ttcttcaaga gatttaatct tattgctaaa cagtgaacaa gaagttataa attataaacc
    16141 taattacgca caattgcgta aattgacaga ctggttaggt ataataatca cagcacaagg
    16201 aagtaataca gattttgtat caagatattt ttgtccagaa ttagattcag aagaccctgt
    16261 tactggttca tcacattgta atcttattcc atactggtct gaaaagttgg gaaaacacaa
    16321 aatggtagct gctcaacttt ctaatcgtgg tggtataatt cagtgtgaag tgttgaagga
    16381 taatactgtg aaaatttctg gtgaagcagt cttgtttatg cagggaacaa taaagattga
    16441 tatttaaata agtataataa ctaatttatt atgaaaaata attaattttc ttagtgttaa
    16501 caataataaa gtagagttaa aaaacttgat ttcacttaaa tcaatatgat agatgttgat
    16561 actaagattg acaagcatga ttaaaaaaga taatatttaa tctataaaga taaaaacaca
    16621 gtctggttgg tagtccagac gcagcaatag ctgtcagtaa cctgcctcct tggttgtccg
    16681 ttctttagaa agtaatttaa ggaggaacta gcaatggaca aaattagtgg aaaattaaca
    16741 gtcttatttg aagaaccatt ttgggtaggt atatttgaaa gacaagatgg taaaaaatat
    16801 gaagcatgta gggtagtttt tggagcagaa cctaaagaag tagaagtgta tgaatttata
    16861 cttgaaagat ttttctcatt agattttggt agcattaaat tagaaaaaaa tgtgaccaaa
    16921 gacaacattg gatacaaaag aatgcagaga aaagtaaaaa aggaacaaga aaaagaaact
    16981 atagggacta aggctcaaaa tgctcttaag cttcaatatg aagaaagaaa acaggataga
    17041 aaaaacctag ctaaaaataa aaaagaagaa gaaaaagaaa gactatttaa tttgaaacaa
    17101 gaaaagagaa aagctaaaca taagggtcat taattttaat taaatatctt tttaataatt
    17161 gctagaaaaa ggtgtataaa atttttaatt ttatacacct taatttattg aaaagaataa
    17221 ggttttatat ttcaattact ttaaaatata aaaatatact aataaaaatt gtaaaaatag
    17281 tataaatggt ataccaaatg taaatttaat gtgtttagtt ttatgtctaa aagaatacat
    17341 tccaattata gaacctatac tacctccaat tactgctata gacataagag tagcttcttt
    17401 tattctccat tcgtttctaa ttgctctttt cttatctatg tacattgaaa agaaagcaat
    17461 agaattaatc actataaaat atattagaat aaaatttttc acagtcttgc ctccataaaa
    17521 atatattata cattataaaa caagatattt gttttggaaa gttaaatttg taattagtac
    17581 atataaaata tttgataaga ataaatataa tattaattgg aaaatttaaa aaaattaaac
    17641 aattagcaaa tagggggtat atttataaga cacaaagtga taaaatctat ttagaaaata
    17701 atgtaaaata acaggaggat aaagcaaaat gaaatttata gtagaaaaag aagtttttga
    17761 taaattagaa aatgtatgct ttggtgtagt agttgcaaaa ggaatagata atactaaaga
    17821 aatagaaaga ataagcaacc ttttagatat aagtatagat agagtagaag attacttcaa
    17881 agataaaaaa gtaaaggaat cagaagaaat tataccatat agagaggcat ttagaagtct
    17941 aggcatgaac ccaaataaat ttatgagttc gatagaagca atgacaacaa gggttgctaa
    18001 gaataagaaa cttcctcata taaatcctat tgtagattta ggaaactcta tttctttaaa
    18061 atatttactt ccaatgggtg ctcatgatat ggattttaga aatgatgatg tatatgttag
    18121 attttctaaa aaaggggata aatttgtacc atttggagaa actgatgttg aattaatgga
    18181 agaaggagaa ttaatttatt cagttggaga catggtaaaa actagaagat ggatatggag
    18241 acaaggagag gaaggaaaaa ttactaatag ctcaaaaaat atattttttc ctatagatgg
    18301 ttttactgat gctaatttag ataaggtaat gagtgcaaga gaagaattag caaaattgtt
    18361 aaaggaaata tttaattgtg aaattaaagt aggttttgta gataaagata atcctgaaat
    18421 ggaaatataa taagttgttg aatacttgtt aagatattat atttacttaa tgatgttaaa
    18481 aaggtattaa aaaagaaagg tcgtagtaaa tttgaaaaaa actataataa agaatgtaaa
    18541 tccaatgatt gaaaataagt ttataggact ttttgaaata gagtataaaa acaaattaga
    18601 tgaagataag gtttggatgg tagcatcaag aaaaagtagt gagcaattaa aaaatattta
    18661 tttgaagaat gaagaagata gtgtagatgc agttgtaata gttgggcttc ataaaagtag
    18721 taaaaaatta attttaataa gacaatttag agtacctatt aatggatata tatacgagtt
    18781 accagcaggt cttgttgatg aaggagagtc tatagatatt tctgttgaaa gagaacttag
    18841 agaagaaaca ggacttactc ttttagaaat taaaaaaaat aaaagtattg ataaagttta
    18901 tttatctcct ggaatgagtg atgaatctgt tgcctttgtt tattgtatat gtgatggtga
    18961 tataacagat gaatttttag aaccagatga agagattgaa gcgcttttaa tttcgcaaga
    19021 agatgcaaaa gagattttac aaagtaatca taagatagac acaaaagcat ttttgatttt
    19081 acaaatgttt gtgagtttag gagctaaatt atttgaatag atatatagaa aaaaggttat
    19141 atagaagagt aaaataataa agtttatttt acttgtataa attataatct ctttttttat
    19201 acaataaagt ataattttgt gtatttttaa ataagtgact tgactcttaa attttcataa
    19261 tatgttataa accaatttaa gatactacaa taattaaaat tccaaaatta tttaacatat
    19321 ttgcaattaa aatgagaaaa ggtaaaggct gaatgattga aaaatttgat gtaaaatagt
    19381 agattagttt aatactatag tttgtagggt taaaaattag aaattgtaag ggagataaat
    19441 tctatgaaaa aattagttat tattttaatt agtatcatta gtgtggttag cttgacagct
    19501 tgtaaaaaaa gcaatgaaag taaatataat aatttaaaca acaaagaatc aggacaaaca
    19561 accattgaaa tggacttaga taaaaattat gatacttcag acccatttgt taatgcacgt
    19621 ttgttttgtg tgtctaatga tatagatgtt ttagacacag agatttcttt taaaatggat
    19681 ggagatagtg gtattgtgga aataaaggac aataaaacag atgaaacttt atggagtaat
    19741 atatggcatg gaagcgttga taatgataca ttcgcaattt ctctttctaa tatacaaaaa
    19801 gagaaagagt atgctatatg ctttactgga attaaaataa atcatgcagt tgtaaaggtt
    19861 acttttgaaa gcaatttagt taaggagaaa gaaaaaccct caaaataaat tccagattgt
    19921 ctataacttg atattcagta cataaaagca gttgatattt atgattgact gctttttctt
    19981 atcaaaaagg ataggttgtg catttttact tgaaatagtt tatgcacatc aaaagttggt
    20041 gagcatgata ttggaaatgg taagcatgaa atcttgataa gacaaggaaa taaatttaag
    20101 ttttcaatta agtatatttc cataatttag ttaataataa gctaattatt atttatattt
    20161 tgatatataa ataataattg tagcaaatct caaaaaatca tatatttttt gaactaaata
    20221 aaaatttatt tttattattt taaattagag gagattcttg aagatatatt atttttaaaa
    20281 ataagtatag tttaaaaatt caaaaaaaag atggcaaaat atgtcccaag ggacattgag
    20341 taaaataaat gatttcagct tatttacact aaaaagtaca tactaaatga ggggtaaaat
    20401 aaagaaaaat gatatactaa ttgtcttttg ataataaaaa aacatggaat ttgattattt
    20461 aatcaagaaa aagtattgaa caaatgattt atttagtgta caataatcat gtaaaaacat
    20521 agtaatggag ggagacattt cataatgaaa attaaggtag gaatgaacgg atttggaaga
    20581 ataggaagag ctgtattaag aatagctcaa gaagaattag gagataatat agaaattgta
    20641 gccataaatg caagagcaac aacagaatct ttggcacatt tatttacata tgattcatgc
    20701 tatggaactt ttagaggtga agtagaggca aaggatgaag acactctagt agtaaactct
    20761 aaagagataa aaatattaag atataatgac ccagaagaat taccatggaa agaactgggg
    20821 gttgatatag taatagaatc aacaggatta tttacacaaa gagaaaaggc agaaaagcat
    20881 attaaagcag gagcaaaaaa agtaattata acagctccag gaaaaaatga agatataact
    20941 atagttatcg gagttaatga gaaagaatat gataatgaaa agcacaatat aatttcaaat
    21001 gcatcttgta ctacaaattg tctagctcct tttgctaaag tattggacga aaaatttggt
    21061 atagtaaaag ggttaatgac tacagtacat tcatatacta acgatcaaag aatattagat
    21121 aaaagccata aagatttgag aagagcaaga gcagcggctg aatctataat acctaccact
    21181 acaggagcag caaaagctgt gtcaagagta ctaccacaat tagaaggaaa attaaatggt
    21241 ttttcactta gagtaccaac accaacagtt tcacttgttg atttagtgtg cgaacttaaa
    21301 gaaaacgtta catctgagca agttaactct gtgttaaaag atgctgctga aggagaatta
    21361 aaaggtgttt taggatattg tgacaagcca cttgtttcta tagattataa aggagattca
    21421 agatcatcta ttatagacgc tctatccaca atggttatag aagacaatat ggttaaggta
    21481 gtttcttggt atgataatga atggggatat tcttcaagaa ctgttgactt agtaaaatat
    21541 gtagctgaaa aattaaaata atatatattt cttaaagcaa attttaaaaa aattttttaa
    21601 aaaaattata taaaactaaa aaaaaagatt atgaaaaata ttgaaggaaa aattttttca
    21661 taatcttttt tctttcatgc aaaaagatac aagttacaac ttatatttgc atattaggtc
    21721 gttagagaag tatatatata tttagtaagg aatcatactt tttataaaat gaaagataag
    21781 atgtcgaaaa cttgcataaa tctgaaaaat ttttattatt aaaatactga tttccatttt
    21841 aaaaatgata aaaaaatgac aaataaagtt gcaaatagta atacttataa gtataattaa
    21901 aaaagagaaa aggttttgtt aaactttata acaaaaaaag aagggatgat tcaaaatgga
    21961 gaagttaaca gacaggagac aggttaaggc agtaagcatg gaaaccttca ttttcctaat
    22021 attactagta gttggttttg ggtatgttgg tagtattatg ggagcaggaa tgatgttcaa
    22081 agtaataatg agtacagcac atgctttact attggagaca gttttcttaa ttatggctat
    22141 ggcagttctt gcaggcgcat taagcgcact actttcagag tttggagtaa tagcgttaat
    22201 taataaaatt ttcgctgtat ttatgaagcc tttatatgga ctacctggtg ctagtatagc
    22261 aggagctata acaacttatt tatcagataa tccagctatt ataccttttg ctaaagataa
    22321 aacattcaca cagtatttta aacaatacca agttccagca ttatgtaact taggaacagc
    22381 ctttggtatg ggacttatct taacaacatt tatgatttct caaggtacag aatatgtact
    22441 tccagcatta attggtaatt taggtgcaat tataggtagt gtcataagtg ttagaataat
    22501 gttgacattt actaagaagt tttataaata caatccggaa gaagataaag caacaggaac
    22561 attagagaaa aaggatgaat ttagagaaat cagagaagga aatgtatttc aaagagcttt
    22621 agatgcaatt cttgaaggtg gtaaaatggg ggtagatatg ggaatggcta taattcctgg
    22681 agtcctagta gtatgtacat tggtgatgtt attaactttt ggaccatcta cagatcctgt
    22741 tacaggtcaa gaagtttata caggagctgc atatgaagga ataaaattac ttccagttat
    22801 tggagataaa cttggattta tattagaacc attatttgga tttacatcac cagaggcaat
    22861 agcattccct ataacttcat tgggagctgt tggcgcagca atgtctctag ttccagagtt
    22921 tataaagtca ggagctataa ctcctaatga tatagcagta tttacagcta tgggtatgtg
    22981 ttggagtgga tatttaagta cacatattgg tatgatggat gcattaaatg ctagacagtt
    23041 ggcaggtaaa gctattcttt ctcatacaat aggtggttta tgtgcaggag cagctgctca
    23101 ctttatattt acactcgtag gataaatgta atcagctaag ataaaaaata atatacaaaa
    23161 aatggttgtt gatatgtaat aagaatttac atatcaacaa ccattttttt ctgactaaag
    23221 ataatttata atacataaac attaagtgga tattagagtt aagttatatt tatgtaaaaa
    23281 gtaacaaaaa tctgtagaaa tttatgacaa aatttagtac actaactata tgttcttatt
    23341 taaaatttaa ggttagaatt aggaattacc tagtagagta agctttgatt aataaaagaa
    23401 tcataaaata aaaatattag tcataaagat tataaataac ttacttaaaa taaagccata
    23461 gaacttacaa aaattaaaaa agcatataaa tatggagggg tacaatggat gaattatttc
    23521 atataggaat tgatgtaggt tctacaacgg tgaaggttgt ggtacttaat aatagtaata
    23581 atatagttca taaagaatat agaagacatt attctgatgt aaaaaaatca gttaaagaag
    23641 tactaaatgg tatatatgaa aaattgggag atataaatac taccattata ataactggtt
    23701 caggtggaat tggaatatca aaaaaattag gtgtaaaatt tgttcaggaa gttatatcta
    23761 gtacaaaatc aatagagtat tttcatcctg aaactgatgt tgtcatagaa cttgggggtg
    23821 aagatgcaaa gataacatat ctaagtggag gaatagacca aagaatgaat ggaatatgtg
    23881 caggtggcac tggagcattc atagaccaga tggcatcact tttaaagaca gatgccagtg
    23941 gattaaatga acttgctaaa gggtataatg ttatatatcc catagcttca agatgtggtg
    24001 tttttgctaa aacagatatt caacctctta taaatgatgg agccaatcag actgatatag
    24061 caatgagtat tttcaatgca gttgtcgtac aaactgtgag tgtcctttct tgtggaagaa
    24121 aaatagaagg aaatgttgcg tttttgggtg gtccattgta ctttttatct gaacttagag
    24181 aagcatttaa aagagtatta gatttaaaag atgaaaacat aatcttccca gaaaatgcac
    24241 aactttatat tgctattgga gcaggattat tatcagtaga tgaagataat atacaactaa
    24301 aatctctaat agaaaaattg gatagtataa agaatattga agatggagaa gtaaatttat
    24361 tagaaccttt atttaaagat aaaaatgagt atgaagaatt ttcaagaaga catgaaaaag
    24421 aaaaaataaa ttatgtggac ataaattcaa taaaaagcaa ttgttattta ggaattgatg
    24481 caggctcaac tacaactaaa gcagcactta ttgatgagga tggaagactt gtatattctt
    24541 attataatag caatgaagga aatccattaa aaacaaccat taaagttata aatgaagtat
    24601 atgatatatt accaaaaaat attaaaatat tgagttcaac tgtaactgga tatggagaag
    24661 gtcttattaa aaaagctctt aaaatcgata atggagagat agagactata gcacattaca
    24721 aagctgctaa atttttcaac aaagatgtag attttatact tgatattggt ggtcaagata
    24781 tgaagtgcct aaaaataaaa gatggagtaa ttgataatat aatattaaat gaggcttgtt
    24841 catcaggttg tggctcattt ttagagacat ttgcaagttc tttatctatg acaatagaag
    24901 aatttgcgca tgaaggtatt tattctcaaa atcctgtaga tttaggttca agatgtactg
    24961 tatttatgaa ttctagagta aaacaagctc aaaaagaagg tgcaagtgtt ggagatatat
    25021 cagcagggct atcttattca gtaataaaaa atgctttatt taaagtaata aagattagag
    25081 atttaaatga gattggtaat aatattgttg tacaaggtgg aaccttttat aatgatttgg
    25141 tgcttagaag ctttgaaaaa ctaattggaa aaaatgtaat tagacctaat atttcaggaa
    25201 ttatgggagc ttttggttca gcacttatag ctaaagaaaa atatgaaagt ggatacaaaa
    25261 catccttgtt atctaaggaa gagttgaata atatcaagct agaagcaagt gtaactagat
    25321 gtaaaggttg ttcaaatcat tgtcttctta caataaataa attttctgat aatgaaattt
    25381 ttatttcagg taatagatgt gagaagggtg aagctattta tggtgataaa aaaataggat
    25441 tagaaaagaa aaaaccaata aatttattta agtacaaata caatagaatt tttagataca
    25501 aaccattgga aaaagaagaa gcaaaaaatg gggaaatagg gataccaaga gtattaaata
    25561 tgtatgaaga ttatccattt tggtttactt ttttcaatgc tatgggcttt agagttgtac
    25621 tttcagatag gtcatcaaaa cagttatatg aaactggtat aactagtatt gcatctgaaa
    25681 cagtttgtta tccaggaaaa ttagttcatg gacatataga aaatttaatt gcaaaaggta
    25741 taaaaaatat attttatcca agtgtaacaa atgaaaataa agaggattca aatgcagata
    25801 attattataa ttgtcctgtt gtaatatcat attctgaagt aataaaaaac aatgtagaga
    25861 atataagaaa taagaacata aattatataa atccatttat aagtctaaat gataaggaaa
    25921 aattgaagaa aagactctat gatgaattat caagacattt ttctgatata aaaataacta
    25981 aagaagaagt taatcatgca gttgataaag ctactgatga acagaattta tttaaaatgg
    26041 agatacaagc agcaggtgaa agagcactta aagaaataaa agaaagaaat atgaagggga
    26101 ttgtgttatg tggaagacca tatcatatag acccagaaat aaatcatgga atgccagagc
    26161 ttataaattc attagatatg gcagtgctta ctgaagactc tatatgtcat ttagccaatg
    26221 tacaaagacc acttagagtt gtagaccaat gggtatatca ttcaagattg tataaagcag
    26281 ctagttttgt aagagataaa aactatttgg aacttgttca acttaattca tttggctgtg
    26341 gattagatgc agtaactaca gaccaggttc aagaaatact taatgaaaaa tcgaagatat
    26401 atactattat aaagattgac gaaggcaaca atttaggagc agctaagata aggatgaggt
    26461 ccttaaaagc agctatgttt gagagagaaa gcaaaaatat agatgtaaaa aatataaagg
    26521 taaacaggat acagtatgct aaaaataatc agataacgaa caaacatact atattagcac
    26581 cacaaatgtc tccaatacat ttccaattcc ttgaaaaagc tgttaactta agtggttata
    26641 atatagaaat tctaaaagat actgatagta gtgtaattga agaagggtta agatatgtaa
    26701 ataatgatgc atgttatcca gctataatag ttgtaggtca gctaataaca gcattaaaat
    26761 ctggcaaata tgatttaaat aacacttctg ttactataac tcagactggt ggaggttgta
    26821 gagctacaaa ttatattgga ttcttaagaa aggctatata cgatgctgga tttaaagatg
    26881 ttcctgtcat tgcacttagt gtaaatggaa tagaagatag tggtataatg gataatatat
    26941 cactaaaatt aatcaataga ctctttatgt ctgttgttta tggtgatttg ctgatgaaag
    27001 tactatatag agttagacca tatgaaaaag ttactggaag tgcaaatgct ctatatgaaa
    27061 aatgggttga tatatgcaaa tcttctcttg ttaaagcaaa aatttctgta tttactagaa
    27121 atataaaaga tatagtaaaa gaatttgata atttagagat actagatatc aaaaaaccaa
    27181 aagtaggttt ggttggagaa atattggtga aattccatcc aattgcaaat aataatttag
    27241 tagatatatt agagcgagaa ggtgctgaag cagtcgtgcc agaccttaca aatttcttct
    27301 taagttgtgc ctttaatact atatacaaac acactcattt agaaggaagt agaaagagta
    27361 gaatgatagg agaggcattt atatacataa ctggaatata tcaaagagta tataaaaaag
    27421 cacttgataa gagtgagaga ttttatgctc cagccaatat aaaaaatgtt gctaaaagta
    27481 cagaaccagt agtgtcatta ggtaatcaaa ctggagaagg ttggctttta acaggtgaaa
    27541 tggttgaatt attaaatgaa ggtgtagaaa acattatttg tatgcaacct tttggatgtt
    27601 tacctaatca cataattggt aagggttcta taaaagaatt aaagaggttg tataaaaatg
    27661 caaatataat acctattgat tatgaccctt ctgcaagtga ggtaaatcaa cttaacagga
    27721 taaaacttat gctttctaag gcttttaaaa atatataaga tttatatttt gatatgttaa
    27781 aagttaatga atttaaaaat atactctata ttaagtatct ataaaaaaga gttatcctat
    27841 tttttagggt aactctttat tagttaaaaa attatatgta tactgatatt aaagggggga
    27901 agtaatatga gtttagtata tgatgtttat aaatcaaaat taggaaattt gtacatatta
    27961 agtgaagatg gttttatagt tagtatatac ataggagatg aaaaatttaa taatttaaaa
    28021 aatgaatgta ttaaaaaagg aattgatata aagagaagta aggaagattt aaaggaagca
    28081 ataagccaga tagatgatta ctttaatgga aaaagaaaag tctttaattt aaatattcgt
    28141 attgaaggga ctgaatttca aaaatcagtt tatagggaaa tgttaaaaat accttatgga
    28201 gaaacattgt gttattcaga tatagcaaga aatataaaca atccaaaatc agtaagagca
    28261 ataggacaag caagtaaaag taataaaatt ccaataataa taccttgtca tagagtagtt
    28321 ggcaaaaaag atattggtgg atatatgggg aatcattctg atttgaaaga gatactatta
    28381 aatttagaaa gacagagtta acttagaaag aaatgttttt attgttaata tttgatataa
    28441 taatagtaaa aagaagtatt ttttacaaaa acaatgaaga atttatttta atttacaaaa
    28501 atgaggggga attttaatgt gtgataaagt tatagcatca aaatatcatg agaagggatt
    28561 taattgtgca gaatcagtta taaaagcata taatgaggaa tttaatacag atataccagt
    28621 ttgtttagga agtggactgg gttcagggtg tggggttgct agtttatgtg gtgcagtaaa
    28681 tgcatcaaat ataataattg gatatgtaaa aggcagaaat cacgaagatg aaagtactaa
    28741 agctaaagta tatgctaaag atttaactac aacagtaaga aaagagtatg gttcagagtt
    28801 atgtatagac ctaaaaaaag atttagttgc ttgtagagaa ataatggatt ttgcatatga
    28861 tagtctaaag gaaactttaa aaaaagaatt ataagaaaat aagctatcaa gtataaatta
    28921 tttgatagct tatttttata gtcgatacta aataaatcaa atatatttta gaattattat
    28981 ttgtaaataa acagcaaata attttaggat ttttatttaa tatagt
//
LOCUS       DAGSWI010000046        27403 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.046, whole genome shotgun sequence.
ACCESSION   DAGSWI010000046 DAGSWI010000000
VERSION     DAGSWI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.046"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            13..312
                     /locus_tag="MJF80_001789"
     CDS             13..312
                     /locus_tag="MJF80_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280456.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            636..2291
                     /gene="ilvD"
                     /locus_tag="MJF80_001790"
     CDS             636..2291
                     /gene="ilvD"
                     /locus_tag="MJF80_001790"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBZ0280457.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRMAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIGNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2635..3264
                     /locus_tag="MJF80_001791"
     CDS             2635..3264
                     /locus_tag="MJF80_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0280458.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFSLIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            3290..4102
                     /locus_tag="MJF80_001792"
     CDS             3290..4102
                     /locus_tag="MJF80_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBZ0280459.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
     gene            4650..5741
                     /locus_tag="MJF80_001793"
                     /pseudo
     CDS             4650..5741
                     /locus_tag="MJF80_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
     gene            5740..6151
                     /locus_tag="MJF80_001794"
                     /pseudo
     CDS             5740..6151
                     /locus_tag="MJF80_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            6288..6941
                     /locus_tag="MJF80_001795"
     CDS             6288..6941
                     /locus_tag="MJF80_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBZ0280460.1"
                     /translation="MNNIYSDIYEKLSTLQWIMKRHQMFCQAESGPFADTSRGQGRIL
                     AMLKIQPKIATKELAYLLGIRQQSLNELLNKMEKNGYVERKPSENDKRVMIVHLTEKG
                     KQVQQPKTDYQNIFNCLLPEELLQMSQYLDRIIEAFQLQNGNALEENNMIDWMAQARE
                     RMGDEHFEQLMSMRERAFGHMRPPKDIPGAERFSENYNGYVPDRDGFQPRNFKPDIK"
     gene            7030..8352
                     /locus_tag="MJF80_001796"
     CDS             7030..8352
                     /locus_tag="MJF80_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBZ0280461.1"
                     /translation="MNQKNENYWLLDAPVTKAIWHMAIPMMLGMSINIIYNITDTFFI
                     GRLNDTAALATISLLLPFTTILMAIGNLFGTGGSTLFSRLLGSENTDRTKQCSATTLW
                     LSFLFGLLTAIISIIFSNYIIRLLGADSNTFAYVKQYLIFYGMGAPFIIANFTLEQLI
                     RGDGKSVESMIGMMISIGANIILDPILMFGLQLGIRGAAIATVIGNAFAVIYYIVCIQ
                     RADNQLSALPKYFRLEKQMLKEIFLVGLSAMLLDILLIVSSLMFNYYALKYGDYVLAG
                     FGISQKLVQIVDLIGMGLYMGVIPLIATAYGARNELRVKEIIKKTALYLALVITCLFT
                     ILFTCRNFIVHCFSNDSDVIRIGAYILTVQLCSSFFAAGAGLLTGIFQAKGEGTPAVV
                     MSVMRGIMLIPAIIFGNYLFKMNGVIFSLLVAEAISCITGLVLYKLKK"
     gene            8465..8676
                     /locus_tag="MJF80_001797"
                     /pseudo
     CDS             8465..8676
                     /locus_tag="MJF80_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8774..9133)
                     /locus_tag="MJF80_001798"
     CDS             complement(8774..9133)
                     /locus_tag="MJF80_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0280462.1"
                     /translation="MRKKKDTHSFDFRPLGLAIREAREKAGLSRNDLGDKVFYGERHI
                     ADIENIGKHPSFHLFHDLVTMFNISVDEYFYPAKNVEKSTVSRQIDSSLDLLSDDELK
                     IIQGTIDGILNSRENKK"
     gene            9457..9831
                     /locus_tag="MJF80_001799"
     CDS             9457..9831
                     /locus_tag="MJF80_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HBZ0280463.1"
                     /translation="MAKTIFEELGGKYERQGDYLIPCLTVPAEEEQPIGTWGQRHLDY
                     LKQYRKVTYTNLLTSGRLNTYLADIDRQAQERFERLIEGMKQAQGITERLKEENALEW
                     TGRMNNIRACAREIVNEEIIFA"
     gene            10075..10998
                     /locus_tag="MJF80_001800"
     CDS             10075..10998
                     /locus_tag="MJF80_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0280464.1"
                     /translation="MKNVYKLEFHSGSKEELLPDFESEFPYIASNAELDKYIGRSAPW
                     HWHRAIELFYMESGTLEYYTPKKKLSFSAGSGGFINSNVLHMTKAVSRTEKNIQRLHI
                     FDTALISGNYGSKIEKKYVMPITANPQIEIYAFRPDNFIHADILDLIKQTFTLCENDI
                     GYEIKIRESLSRIWLKLFELFSASFDGKGKYDKNDERLKKMLIYIHEHFADKIAVSEL
                     ATVACLSERECFRLFQSYLHTTPTKYIQSYRLQEACEMLINGQDTITDIGHACGLGNS
                     SYFSKIFREYQNCTPIEYRKKWQDSHILCPK"
     gene            11074..11664
                     /locus_tag="MJF80_001801"
     CDS             11074..11664
                     /locus_tag="MJF80_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBZ0280465.1"
                     /translation="MFYEAKDFLETADKETLHIISNARKLTREYYFTDYDNTKRKNEI
                     LKELLGAVGKNVAIDVPLHCDYGKNVFLGDDVVINMNCTFVDNKPIRIGSRVLIASNV
                     QFYTSSHPVLPQERFVSDWEETGTTFFRTYARSIEIQDNVWIGGGSIILAGVTIGENS
                     VVGAGSVVTRSIPANCVAVGNPCKVIRCFTEKRGKA"
     gene            11664..12284
                     /locus_tag="MJF80_001802"
     CDS             11664..12284
                     /locus_tag="MJF80_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016304801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBZ0280466.1"
                     /translation="MKYQHIVFDIDGTLINTEHAILHSLQDTLEECIHEKVPVKQLLF
                     VMGITGEDALRQLKINNIPLSLKLWEENIKKYSNTITVYDGIQELLNSLSECDCKIGI
                     VTSKTKAEFKDDFDRFGLKHYFKTIICADDTTGHKPSTEPLLKYIEISGAYNEQILYI
                     GDSKYDSLCAKNAEVDFALAMWGSHTRELEADYFIETPNDLLGAIK"
     gene            12326..12523
                     /locus_tag="MJF80_001803"
     CDS             12326..12523
                     /locus_tag="MJF80_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBZ0280467.1"
                     /translation="MNIEKWILCPVCGGKTRNKIREDTVLINYPLYCPKCRQESLIVA
                     KNMQISIVIEPNAHTYKAVNY"
     gene            12555..13175
                     /locus_tag="MJF80_001804"
     CDS             12555..13175
                     /locus_tag="MJF80_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008751343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBZ0280468.1"
                     /translation="MDNNEKWLQWAVELQSLAQAGIFYGKDVFDKERYERIREIAAEM
                     ISYKSEISMDKVKNLFCNEIGYQTPKIDCRAAVFQNNKILLVQEKNGTWSLPGGWVDV
                     NVSVKENVMKEVKEEAGLDVMADMVIAVQDREKHNLPVYAYKVCKIFILCSVIGGEFK
                     SNIETISSNYFGMNELPILATEKNNEEQIKMCFEAYQSENWKTFFD"
     gene            13757..14224
                     /locus_tag="MJF80_001805"
     CDS             13757..14224
                     /locus_tag="MJF80_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBZ0280469.1"
                     /translation="MELSYTDFCRRRQADQSYMDKLFIPVQGCLLEVVREQYADFYKE
                     KERWRYLKKLDTNHKLLSLEGFTDSEGNVLDFVIDETVDVAETVVHAVMVDSLKAALS
                     LLSGREQALINAIFFEELSEREVGLRLGVTQSVVNKRKSKILAKLRKIIENKI"
     gene            14313..14738
                     /locus_tag="MJF80_001806"
     CDS             14313..14738
                     /locus_tag="MJF80_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBZ0280470.1"
                     /translation="MAYNHGREDRKWRIWKEAEEKILRECGVDEETIEQIRTDDRADF
                     NSNRRFYRWASDFGEYLEGMADREKQAEVKSVSDLLDEIEDETLYLALVTVDRRTLQI
                     VLLKMQGYSTKEIAPLVHLTAGAVYARLNHLRKKLRKIL"
     gene            14821..15294
                     /locus_tag="MJF80_001807"
     CDS             14821..15294
                     /locus_tag="MJF80_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBZ0280471.1"
                     /translation="MAYGAKTYKLREESTESGARYFISFKDGQREHHELEVSEQLFIE
                     FRQMERRNRNLQQWNQRHREFNEVWDETLYRRALRVPKTLDERMIEKERNEMFLKAVA
                     RLPEIQRRRFLLYYEYDFNFYQIGAMEHCTASAVQKSVSVAREKVKAEMRKYLQS"
     gene            15445..16476
                     /locus_tag="MJF80_001808"
     CDS             15445..16476
                     /locus_tag="MJF80_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015512777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBZ0280472.1"
                     /translation="MQKLQTVNADTLLYEPLEKPSFVVDGLIPTGLTLFCGSQKIGKS
                     WLMLKLCLCVSQGIPLWDMPTREGDVLYLCLEDTFCRIQDRLFRLTDEASGRLYFAVA
                     SDKLSDGLIVQLEDYLKEYPDSRLIVIDTLQKVRTASKDNAYASDYGDISLIKDFADR
                     HSLAVIVVHHIRKQNDSDVFNKVSGTTGLTGSADATFVLEQESRASNAAKLYVTGRDT
                     PYQEFTLRFRDCSWELVERKEQEQLAKEAIPDVLFRLVDFMQDKEEWTGTATELLAAM
                     GETETVPTVITKWLNEYRTTFLSENHICYQYSRKKDGRQIALARQTGDSGDSDDSGIG
                     IPPVTVIDT"
     gene            17000..17965
                     /locus_tag="MJF80_001809"
     CDS             17000..17965
                     /locus_tag="MJF80_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBZ0280473.1"
                     /translation="MPYAILRFQKRKAGGVAACERHNERKKEAYKSNPDIDMERSKNN
                     YHLIAPPKYTYKKEINRMVAEAGCRTRKDSVMMVETLITASPEFMNQLPPEEQKEYFQ
                     TALDFISERVGKKNILSAVVHMDERTPHMHLCFVPLTPDNKLSAKSILGNQKSLSEWQ
                     TAYHERMFARWSELERGQSSMETKRKHIPTWLYKLGGRMDKQYEEIVSALSDINAFNA
                     GKKRDKALELLSAWLPDVEKFSKEISKQSAYIDSLKERIGQESDYAGRMRDEKYEQER
                     KVQKANQRIFELQKTNQQMERLLKKIPPEVLEELQKSNRNRARER"
     gene            17974..18828
                     /locus_tag="MJF80_001810"
     CDS             17974..18828
                     /locus_tag="MJF80_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBZ0280474.1"
                     /translation="MKKQDFKVLKTADLYPFPDNPFHVVEDEMLSELAESIKEFGIVT
                     PIITRPKEDGNGYEIIAGQRRVRASELAGINTIPAFVLPLDRDRAIITLVDSNLQREN
                     ILPSERAFAYKMKSEAMKRQGFRTDLTSSQVGTKLRTDDKVAQGFGVGRMTVQRFIRL
                     TELIPPILQMVDEGKIALTPAVELSFLKKGEQENLFATMESEEATPSLSQAQRMKSLS
                     QSRRLNMDAIFAIMTEEKGNQKETVKIGMERLKKYFPKGTTPKQMEDTIIKLLERELQ
                     RKRGRDSR"
     gene            18907..19137
                     /locus_tag="MJF80_001811"
     CDS             18907..19137
                     /locus_tag="MJF80_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280475.1"
                     /translation="MKEIQYEIVKEIAVLSTSESGYTKEINLISWNGKEPKYDIRSFS
                     PNREKCGKGITLTADEAAALLKALQEELNSGD"
     gene            19209..19400
                     /locus_tag="MJF80_001812"
     CDS             19209..19400
                     /locus_tag="MJF80_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBZ0280476.1"
                     /translation="MGEDKKANKKRKRIVARQPVQMIVSREYVGTQTVAEAFIPIISE
                     DIRRKIAEGDTFDNEDISA"
     gene            19455..21314
                     /locus_tag="MJF80_001813"
                     /pseudo
     CDS             19455..21314
                     /locus_tag="MJF80_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567773.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            21931..22869
                     /locus_tag="MJF80_001814"
     CDS             21931..22869
                     /locus_tag="MJF80_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBZ0280477.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            23345..23773
                     /locus_tag="MJF80_001815"
     CDS             23345..23773
                     /locus_tag="MJF80_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBZ0280478.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGDMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(23926..24132)
                     /locus_tag="MJF80_001816"
     CDS             complement(23926..24132)
                     /locus_tag="MJF80_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280479.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            24454..25110
                     /locus_tag="MJF80_001817"
     CDS             24454..25110
                     /locus_tag="MJF80_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0280480.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTIRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            25194..26273
                     /locus_tag="MJF80_001818"
     CDS             25194..26273
                     /locus_tag="MJF80_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBZ0280481.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQQFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAVAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLSELKNTTIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCILLEEIAPKLAK"
BASE COUNT         9353 a         4473 c         5999 g         7578 t
ORIGIN      
        1 aggaggaatt ttatgaaatg gattaaatgg tatagtatta catgcatatg tatttttata
       61 gtagtagctt tttatatgtt tatatttcct aataaaatag aaactattga tacttcatct
      121 gcatatagct ttgttgaaaa gaaagtacca aatagtgctg tgtatcaagg atacaaaaag
      181 aatccagttg atggtactac tacaatttat tacagctatg ataattcaac tcatatagtt
      241 agattatcac atccagaaga ctattcaagg aagattaatt gggataaagt ttcaaacatc
      301 agatttgatt aatctttaat atttataaaa gtctatatat ctaaggcaaa tatattatta
      361 atcttattca tagttgagca ctttctatta tagaagtgcc tcttctgatt tatatcataa
      421 aatattactc aacaatttgt aaaatatatt agtttataaa atatattgaa aagataatat
      481 tatataatac gattgtgtaa gatattgata aaaaatcgta ataaattttc attagtatat
      541 gctaatattt ttttacaatt ttcgtaatta attaatagaa aaatatatta taataatatt
      601 atgaaaattt aaaaaagtaa gaaagaggta atattatgag aagtgatata aaaaaaggaa
      661 tagaaggagc tccaaagaga gcgttaatgt atggaatggg tcttactaaa gaagaaattg
      721 aaagaccatt aataggtata gtaaatgcac aaaatgaggt gattcctggt catctgcatc
      781 ttgatgaaat agcagaagct gcaaaaaatg gagtaagaat gagtggtggt ttgccattag
      841 aatttcctgc tataggggta tgtgatggta tagctatggg gcatgtcgga atgaattatt
      901 ccctggcttc aagagaatta atagcagatt caatagaagc tatggctatg gcacatggtt
      961 ttgatgcttt ggtacttata cctaattgtg acaagatagt gccaggtatg ttaatggctg
     1021 ctgcaagact taatatacca agtattgttg tgagtggagg gcctatgctt ccaggtaaaa
     1081 aaaatggaaa agtatatgat tttaactctg caatggaggg tgttggagca tgtaaagatg
     1141 gaacagtaag tgaagaagaa ttagaagcgt tagccatgaa ctcttgtcct ggatgtggtt
     1201 catgttctgg tcttttcaca gcaaatagca tgaattgttt aacagaagct ttaggcatgg
     1261 ggataccata taatggtact gctgcgtcac attctggaga aagaaaaagg atggcaaaat
     1321 atgcaggtat gtatgttatg gagttactta agaacgacat aaaacctaga gatattttaa
     1381 caatagatgc ttttaaaaat gctatagctg tggatatggc aatggctggt tctacaaata
     1441 cagtacttca cttacctgca atagcttatg aatcaggaat agagcttaac ttagattttt
     1501 ttgatgaaat aagtgaaaaa actccttgtt taacaaaatt aagtccaagt ggaaaacatc
     1561 atattgaaga tttacatatg gcaggaggaa taccagctat aatgaatgaa ctttcaaaga
     1621 taaatggaat aaatttagat tgcaaaaccg taacaggcaa gactataggg gaaaatatag
     1681 gaaattgtga aatagaaaat gaagaagtaa tacatacatt aaagaatcca tatagtaacc
     1741 aaggtgggct tgcaatattg aaaggaaatc ttgctctaaa tggagctgtt gtaaaaaagt
     1801 cagcagttgc agaagaaatg ttagttcatg aaggaccagc aagagttttt aattcagaag
     1861 aagaagctgt aaatgctatt tttggtaaaa aaataaacaa aggtgatgtt atagttataa
     1921 gatatgaagg tccaaagggt ggtccaggaa tgaaagaaat gctatctcct acatcagcag
     1981 ttgcaggaat gggacttgat aagcatgtag cacttcttac tgatggtcgt ttttcagggg
     2041 caactagagg agcatctata ggccatattt ctccagaagc tatggaaggt ggtttaatcg
     2101 ggttagttga agaaggagat ataatttcta taaacatacc agacaaaaaa ttagagttaa
     2161 aagtagatga agttgaaata gaaaatagaa aattaaaatt taaaccttta gaaccgaaaa
     2221 taaagcatgg atacttaagt agatatgcta aattggtaac atcagcaaat acaggagcag
     2281 ttttaaaata gtagactata tttatgtaaa ttagaaaaag ttaagaaaat attaattgga
     2341 tatcttaggt gttatagcac taatgaggta tcctttttat tttgtatgaa aaacacagca
     2401 aaatcttgat tatctttgaa aacaatacat ctctattata aggaatacga gtacagcata
     2461 gagatgtatc ttttagacct tgtttaggta atacaacttt ttaaaagttt gatgtggaat
     2521 gcttttcgtt ccatctgaac tatgaagaaa ttgaaagtca gttaattctt gacataataa
     2581 atattaaaat gtataataaa aaacaaacag acagtctgtt tttaggaggg aaatatgaat
     2641 agagaagaaa aaagtaaaaa tagtaaagaa aaaattatcc aatcagcatt ttcactattt
     2701 tcgtctaaag gatatgattc aacatctaca caagatatta tcaatttatc tggtctatct
     2761 agaggtgcaa tgtatcatca ctttaaaact aaagaagata tactgagaag tgtcacaaaa
     2821 gaactttact cacaaatgaa taatttttta gagcatcttg ttgctgatga cacccttaca
     2881 gcaaatgaaa aaataataga attggttgtt catagtgcga atgattacac acgtagaaaa
     2941 atggtacatt gtagctggtt agaaaaaatc ccattctctt taatagagga agttcgtaat
     3001 ctcaacaatg tagttgcacc caatattgct aagataatta aacaaggtgt tgaaaataaa
     3061 gaattttctt gtgaatatcc agaggaatta gctgaaatgc tcgtttttag tattgatatt
     3121 ttacttgacc ctgtattatt taaacgtgaa tacagtgaag tatgtaatag gttggatttt
     3181 ttattattca tgttaaaaaa aatggatatt cctctaattg atgagtacgg tattcaaaaa
     3241 tttaaagatt tatttaaaca atgataatag gaaaagtgag gaatagacta tgttttataa
     3301 tgcaaaaagc tgtcaaatga tgataggaag taatacagtg aattatattg aatttggtag
     3361 tggtaagaag acactaatta ttttaccagg attaggagat ggtttatttc ctttacatgg
     3421 aaaaatacaa gcgattgctt ttgcttttag atataagcaa tttgcaaaag attataaggt
     3481 ttatgtattt agtagaaaaa atcaaattac agaaaaatat tccacaaggg atatggctaa
     3541 agaccaagca gatataatga aaaaacttgg aattatgaaa gcagaagtaa tgggtgtttc
     3601 tcaaggtgga atgatagctc aatatctggc aattgattat cctgagttag ttgagaaact
     3661 tgtgttggca gttacttctt caaagcaaaa tgatactatc caaaatgtta tttgtagttg
     3721 gattgatatg gcgaagaagc agaattacaa tgatttaatg attgacacag caaagaaatc
     3781 ctattctgaa agatatttaa aaaaatacca attatttatt ccttttttag gaaaagtagg
     3841 taaaccaaag gattttaaac gatttattat tcaagcgacc tcttgcatag aacataatgc
     3901 cttttccgaa ttaaataaaa taacatgtcc aaccttaatt ataggaggtg ctaatgataa
     3961 aattgttgga aataatgctt cttttcattt agcagaaaaa attaaaaaaa gcgaaatatt
     4021 tatctatgag ggattgggac acgctacata tgaagaagca caggatttta atgaaagagt
     4081 tttagagttt ttaaataagt aaacaccttt gttaaaaata tacatatgaa gattggaaat
     4141 ttaatgttaa aaatagaaac atgaaaatat gcttaactgg tatttttact attcataacc
     4201 aatttttgat acattatcta ctataaatac aaatatagct tcaatgtgat tatatattgg
     4261 tgtattggta aagcacttat acaaacagag gaattttgta aattcatatt atacccacat
     4321 ttgttaactt atgaaaatat aatcaaaatt tttatgagat ttttatataa aaaacgccct
     4381 aaaaatctta ttatccccat aaacactgta tctaaaagca tattcaatag gaaataaatc
     4441 gtgatattac tacgaattta ctactaatga atgagccttg atacgtctta tttcccagat
     4501 atgcaaagat atggcatggc acatcagtaa aaattgaata cttatataga ctatggaacg
     4561 tacacttttg gcgtttcttt tctatttcca gatgttcttt tcagagcgtc tttttttcat
     4621 acccaaaatc gaaaggagaa agagaaaata tgaataagct agtaaagcga ttgctgacag
     4681 ggacgctcgc cattgcaacc attcttaccg cattgcctgt gacggtggtt catgcttctg
     4741 gcaatcaata ctggacagaa tcagcagaac gtgtcggcta cattgaacat gttatgaatg
     4801 atggttctat caaatccaca ttaaatgagg gacacatgaa agttgagggc gaaactgcct
     4861 attgcgtgga catcaataca aatttcaaaa atggatataa aacaaggtat gacgcaagct
     4921 cccatatgag tagcgatcag attgcggaca ttgctctttc cttagagtac atcaagcaat
     4981 atactgcttc tcatacaaac ttgaattaca agcagggtta cttattggaa cagtgtgttg
     5041 tctggcagag attgagtgaa cagctcggct ggcaatgtga taacgtcaga gcttcctata
     5101 atgaaatctc acaggcggta cagaataaag tttacgctgg tgcgaaagta tttgtgaaag
     5161 caaataaggg atgctatgaa tgtggcggtt acatctacac tggcgaagga caggacattg
     5221 gacagttctg ggcgaagttg aatgtgggaa atgaaaaggt caaaaagact tcttccaatt
     5281 ccaccctcta cagatggcaa tgtcaattat tcctttgagg gggcaacatt tggcgaatcc
     5341 catattgtag tcacagataa aaacggtcag ttctctactg cttcaagttg ggctttccgt
     5401 aaggtaaata caaatgctgg aaaatccagt gaggatggtg tatggtttgg tacttctgaa
     5461 ccagacgaca gtaaaggtgc attacctatg tgattgagga attaaagtgt gattccaacg
     5521 ccggatttaa gctgattcca gcttttgagg tggtcgtatc cagaaaaaaa gtgaccgtag
     5581 atttagggac acttactgat gaatatgaaa aagaaatcac aatccatacc acagctaccg
     5641 acaagaaaac aggcgaaaaa atgattgttg ccggaaaaga catcaagatc gtggacaaag
     5701 tcacacttga tggcttggaa actggcagtc acagaaaatt acaccgtgac cgttcatgtg
     5761 tatacagatg gtgaaatggg tcattaccca gaatcctacc cttgctccgg tggtacagaa
     5821 atcaaagtat gaaccgaaag cacagggagc agatgtcagc gtcagctccg atacgttcaa
     5881 agacgctact actttcttgg aaacattctt taaactctat ccgacagcta cagaaaaaga
     5941 acttgcctat tatgtcaaag acggtgtgct tgctcctgtt tctggcgact atgtattttc
     6001 ggaactggta aatcctgtct ttaccaaaga tggcgataat ctcaaggtca gtgtgtccgt
     6061 gaaatatctg gataacaagt cgaaaatgac acaaatctca cagtatgagc ttgtgcttca
     6121 caaggacgat aattggaaaa ttgtaggata aatattacag cagaccagct atgacaattt
     6181 caaatacata tactaagttt taaaaatata catgtaccct attgactttt tgggaaggta
     6241 cctgtatatt atttgttaaa ggtacctgtt gaaaaggagg tcatactatg aataatattt
     6301 attctgatat ctatgagaaa ctatcaacct tgcaatggat tatgaaacgc catcagatgt
     6361 tttgtcaggc agagtctggt ccatttgctg atacatcacg aggacaagga agaattcttg
     6421 ctatgttaaa aattcagcca aaaattgcaa caaaagagtt ggcatattta ttgggaatac
     6481 gccaacaatc cctaaatgag ttgcttaata aaatggagaa aaatggctat gtagaacgaa
     6541 aaccatctga aaatgataaa cgcgttatga ttgttcattt gacagaaaag ggaaagcaag
     6601 ttcagcaacc gaaaacagac tatcaaaata tctttaattg tctacttcca gaagaattgc
     6661 tacaaatgtc gcaatattta gatcgtatta ttgaagcttt tcaattgcaa aatggaaatg
     6721 ctttagaaga aaacaatatg attgactgga tggctcaggc aagagaacgt atgggcgatg
     6781 agcattttga acagttaatg tctatgcgtg aaagagcttt tggacatatg agaccaccca
     6841 aagatatacc gggagctgaa cgcttttctg aaaactataa cggatatgtt ccagatagag
     6901 acggatttca gccaagaaac tttaaaccag atatcaaata acaaaaggca ctgtaaaatt
     6961 acagcaataa gccctatgga gattttttga actccatagg gcttattttt tgaaaaggag
     7021 acgaacaaaa tgaaccagaa aaatgaaaat tactggcttt tagacgcacc tgtcacaaaa
     7081 gctatatggc atatggcgat tccaatgatg cttggaatgt caataaacat tatttacaat
     7141 attacagata cattcttcat aggcagatta aatgacacag cggctcttgc cacaatctca
     7201 ctgctgttac ctttcacgac cattttaatg gcaattggga atttgtttgg aacaggtgga
     7261 agcactttgt tttcacgact gttaggaagt gaaaatacgg acaggacaaa acaatgttca
     7321 gctacaacat tatggctatc tttcctgttt ggactattga ctgcaataat atccatcatt
     7381 ttcagtaact atattatccg acttctcggt gctgatagta atacttttgc ctatgtcaaa
     7441 cagtatctta ttttttatgg aatgggtgct ccgtttatta ttgccaattt tacgctagaa
     7501 cagttaatta gaggtgacgg taaatctgta gaatctatga ttggaatgat gataagcatt
     7561 ggtgctaata tcattcttga cccaattctg atgtttggat tacagcttgg tattcgtggg
     7621 gcagccattg ctacagtaat cggaaatgct ttcgctgtta tctattatat tgtctgtata
     7681 caacgagcag acaatcagtt atctgctctt ccaaaatatt tcaggcttga aaaacaaatg
     7741 ctaaaagaaa tttttttagt tggattatct gcaatgttgt tagatattct tttgattgtt
     7801 tcaagcctta tgtttaatta ctatgcacta aaatatggag attatgtgct tgccggattt
     7861 gggatttctc aaaaacttgt gcagattgtc gacctaatcg gcatgggact ttacatggga
     7921 gtaattccac ttattgctac tgcctatggt gcaagaaatg aacttcgtgt gaaagaaatc
     7981 attaaaaaga ctgctctcta tttagcacta gtaattacat gtttgtttac tattctattt
     8041 acatgcagaa actttattgt tcattgtttt tcaaatgatt cagatgtaat tcgtataggt
     8101 gcgtacatct taaccgttca actctgttct tctttctttg cggcaggagc gggtcttcta
     8161 acgggaattt ttcaggcaaa aggagaagga actccggctg ttgttatgtc cgttatgaga
     8221 ggtattatgc tcataccagc aattatcttc ggcaattatc tctttaaaat gaatggagtt
     8281 attttttctc tgcttgtagc cgaagctatt tcatgcatta cagggcttgt attatataaa
     8341 ttgaaaaagt aatgctgaac tccatcagac ttgttaacaa agttagtgat ggttataaaa
     8401 atcatcaaca ataaattaag agatttctat agaatgagta tagattggta ggagctggaa
     8461 aaatatgatt gggcttaaaa aaagaaatat aaaaaaatgt tgaaaactgg tgatagagct
     8521 ggagatgtat cattagctga tattcgggct aatatagaag ccactattga tgaagacatg
     8581 aacagtccag ccccagaggt acaggcaaat ttcaaaaagt attttggcaa taaacgtcct
     8641 acaccagaag aatatattta caagattaca aaaaaacaaa agtttgattt acgactattt
     8701 gttgcctgcg gagtatatca acaaataagc gttaaagaat ctgcacagaa aaacagagaa
     8761 aataagaagc gaatcacttc ttattctctc tgctgttcaa gataccatca atcgtacctt
     8821 gaatgatttt taattcgtca tcactcaata aatcaagtga cgaatctatc tgacggctaa
     8881 ctgtgctttt ttccacattc ttcgctggat agaaatactc gtcaacagat atattgaaca
     8941 tggtaactaa atcatggaat aaatgaaagc tagggtgttt cccgatattt tcaatatctg
     9001 cgatatgacg ttctccgtaa aagactttat cgcctaaatc atttctggaa agccctgctt
     9061 tctctctggc ttctctgatt gcaagtccta aaggacgaaa atcaaagctg tgcgtatctt
     9121 tcttttttct catttttaat cacctagcat gagtttatac tattttcaca ggtttgctct
     9181 gctcaaggag caatttgtga ttctaagtaa aacaggcagc cggcaccgac aaccgaattt
     9241 attccttttt tttagtttat catcagccct tgagcatgaa ttaaaaactc atatatcaga
     9301 tgttttaaat acagtctatt tgttattagt gcagataggc tgttttcata ttttcaaaaa
     9361 gtttttcaat tctgcaacaa atcacaccct gctgtacaga tgtagaacgg attttgcgga
     9421 cattcaaaat aaaaaagaat gtggaggtaa cagacaatgg caaagacgat ttttgaggaa
     9481 ctgggcggca aatatgagcg gcaaggagat tatttaatac cgtgcttgac tgtacccgcc
     9541 gaagaagaac agccgatagg cacatgggga cagcggcatc tggactatct gaagcagtac
     9601 cgaaaggtta catacaccaa tcttctcaca agcggcaggc tcaacactta ccttgccgac
     9661 atcgacaggc aggcgcagga acgctttgaa cggctcatag agggcatgaa acaggcgcag
     9721 ggcataacag aacgcctaaa ggaagaaaat gccctagaat ggacaggaag aatgaataac
     9781 ataagggcgt gtgcgaggga gattgtgaac gaagaaatca tttttgcata agtagagaag
     9841 ggcggcagag tgtaaaagct ctgctgtcac tgttttaaat taacaatctg ttttcgttgc
     9901 atatatgaca ttttcaaata aatcgacaga gagctgccct atgctattga tacaattcat
     9961 tggaacccct atactatatg gtgtatagct atttcccaca aaagtgagaa atctgctgat
    10021 gtgaaaaaaa tctgaaaaca aactgaatga tacaatgtga aagaggaatt ttgtatgaag
    10081 aatgtatata aacttgaatt tcattcggga agcaaagaag aattattgcc tgattttgaa
    10141 tctgaatttc cgtatattgc atctaatgca gaacttgaca aatatattgg gcgttctgcg
    10201 ccgtggcatt ggcatagggc aatagagctt ttttatatgg agagtggcac attagaatat
    10261 tacacgccga aaaagaaatt gtctttttcg gcaggttctg gaggttttat caattcaaat
    10321 gtccttcaca tgactaaggc tgtttctagg acagaaaaaa atatacagcg gctgcatatt
    10381 tttgacacag ctcttatttc tggaaactat gggagtaaaa ttgagaaaaa gtatgttatg
    10441 ccaattactg caaatcccca aattgaaata tatgctttcc gccccgataa ttttatacac
    10501 gcagatatat tggatttgat aaaacagact tttaccttat gcgagaatga cattggatat
    10561 gagataaaga taagggaatc cctgtcccga atatggctaa agctttttga gctgttttct
    10621 gcttcatttg atgggaaagg aaaatatgat aaaaatgacg aaagacttaa gaagatgtta
    10681 atttacatac acgaacattt tgcggataaa attgcggttt cagaacttgc cacagttgca
    10741 tgtttaagtg aaagggaatg tttcaggctg tttcaaagct atttgcatac aacgcccaca
    10801 aagtacatcc aaagttaccg tttacaagaa gcttgtgaaa tgctcataaa tgggcaggac
    10861 acaattacag acattggaca tgcctgcggg ttagggaaca gcagctattt tagcaagatt
    10921 ttccgtgaat accaaaactg cactcccata gaatatcgga agaaatggca ggatagtcat
    10981 atcttatgtc cgaaataaaa tagataaata aataattctg cgttataatg atatatagca
    11041 catattttgt tatatcattt taaggagaac gctatgtttt acgaagccaa agattttttg
    11101 gaaaccgcag ataaggaaac tttgcatatt atttccaatg caaggaaact tacaagggaa
    11161 tactatttta cggattatga taatacaaaa agaaaaaatg agattttgaa agaattgttg
    11221 ggagctgttg ggaaaaatgt tgccattgat gtcccgcttc attgtgacta tggaaaaaat
    11281 gtatttcttg gtgatgatgt cgttataaat atgaattgta catttgtgga caataagccc
    11341 atacggattg gcagtcgggt attgatagca tctaatgttc agttttatac atcttcccat
    11401 cctgttttgc cacaggagcg tttcgtttcg gactgggaag aaactgggac aacatttttc
    11461 agaacttatg cccgttccat tgaaatacaa gacaatgtat ggattggagg gggaagcatt
    11521 attttagctg gcgtaacaat cggtgaaaac agtgtggttg gagcaggaag tgttgttacc
    11581 cgttcaattc ccgcaaattg tgttgctgtt gggaatccgt gtaaagttat tcgctgtttt
    11641 acagagaaaa gaggaaaagc ataatgaaat atcaacatat tgtttttgac attgatggga
    11701 ctcttatcaa cacagagcat gcaattttgc actctttaca agacacatta gaagaatgta
    11761 tacatgaaaa agtacctgtc aagcaattgt tatttgtaat gggaattacg ggagaagatg
    11821 cattaaggca gttaaaaatc aataatattc cgttgtcttt gaaactatgg gaagaaaaca
    11881 taaaaaaata cagtaataca ataactgttt atgatggaat ccaagaatta ttaaacagtc
    11941 tatcagaatg cgattgtaaa attgggattg tcacctcaaa aacaaaagca gagtttaaag
    12001 atgattttga taggtttggg ctgaaacatt attttaagac gattatttgc gctgatgata
    12061 caacagggca taaaccatcc actgaacctt tgttaaagta tatagaaatt tctggggcat
    12121 acaacgagca aatattatac ataggtgata gcaaatatga cagcttatgt gctaaaaatg
    12181 cagaagttga ttttgcattg gcaatgtggg gaagccatac cagagagttg gaagccgatt
    12241 acttcattga aactccaaac gatttactag gcgctataaa ataagcagga atgataagaa
    12301 aataattggg caaagaggtg gttttttgaa tatagaaaag tggattttat gccccgtttg
    12361 cggtggcaag acacgtaata aaattagaga ggatactgtt ttaataaatt atcctcttta
    12421 ttgtcccaaa tgcagacagg aaagtttgat tgtagcaaaa aatatgcaaa tcagtattgt
    12481 catagagcct aatgctcata catacaaagc ggttaattat tagaatgata ttaacttgca
    12541 gacaaggagg acacatggat aataatgaaa aatggctgca atgggcagta gaattacaaa
    12601 gtctggcaca ggcaggaatt ttctatggaa aggacgtttt tgataaagaa cgctatgaaa
    12661 gaattagaga aattgcagca gaaatgattt cttataaatc agaaatttca atggataagg
    12721 tcaaaaatct attttgtaac gaaattgggt atcaaacacc taaaattgac tgtcgtgctg
    12781 ctgtttttca aaataacaaa attttgcttg tgcaagagaa aaatggaaca tggtcattgc
    12841 ctggaggttg ggttgacgtt aatgtttcag tgaaagaaaa tgtgatgaaa gaagtaaaag
    12901 aagaagctgg tttagatgta atggctgata tggttattgc cgtacaggac agggaaaagc
    12961 ataatttacc agtttatgcc tataaggtgt gcaaaatatt tatattatgt tcagtcatag
    13021 ggggcgagtt taaatccaat atagaaacca tatcgagtaa ctactttggg atgaacgagt
    13081 tgcctatcct tgcaacagaa aaaaataatg aggaacagat aaagatgtgc tttgaagcat
    13141 atcagtctga aaactggaaa acattttttg attagtattt tttagaaaga gccagacact
    13201 ttagatgcag agccaatgaa cttgtgaaat agaatcacag tttattggct ctatttcatt
    13261 tcataagatt ttaaggcaaa cgcccaccaa ataaaaaaaa cgaggttcgg tgggcggtat
    13321 tgctatgccc gaaaagactt aacagacact ctccagacct gttttagagt ttggagggat
    13381 ttttttatgt tcttttttcg ggtagttatt catctgctca tgcaacgcag agtttaccca
    13441 tacatagcat atcggggaat ggcaggaagc cagtcaaaaa agttcctgct tatgcaacgc
    13501 tacttcacgc cttgaaacca gaagtagaat ctccacctaa cagaatatgt atctcctata
    13561 accgtagaag tcacagaact tttgcggttt ttcttttgtc gttttttatc ggaatgtgcc
    13621 gcagggctag ttcttgtgtt ggctgtcgtt cgccgccttt tccatctgga ttttaacaat
    13681 tcaaaaattc agatgggagg tacttacggt gaagtatgca ccaagaaaag tatatatcaa
    13741 gaaaaatggc ggttatatgg agttatccta tacggacttc tgccgccgca ggcaagccga
    13801 ccagagttac atggacaagc tgtttatccc cgtgcagggc tgtctgcttg aggtagtgcg
    13861 ggaacagtac gcagattttt acaaggagaa agagcggtgg cgttatctga aaaagttgga
    13921 cacaaaccac aagctgctct cattagaggg ctttacggac agtgagggaa atgtgcttga
    13981 cttcgttatt gatgaaacgg tggacgttgc ggaaaccgtt gtccatgcgg tgatggtgga
    14041 cagtctgaaa gccgccctgt ctttattgtc gggcagagag caggcattga taaacgccat
    14101 cttctttgaa gaactttccg agcgggaagt cgggttgcgg ttaggcgtga cacagagcgt
    14161 tgtgaacaag cgcaaatcca aaatccttgc gaagctgagg aagataatcg aaaacaaaat
    14221 ttaaggcgtt cagccccctt gttttttcct ttgggaaaat gagggggctt ttccctgccc
    14281 cctcaatcaa ttctgattgg aggaaaaaaa gaatggcata caaccacgga cgggaggaca
    14341 ggaaatggcg catctggaaa gaagcggaag aaaagatttt gcgagagtgc ggcgttgatg
    14401 aagaaaccat tgagcaaatc cgcacagacg acagggcaga ttttaattcc aacaggcggt
    14461 tttaccgatg ggcgagcgac ttcggcgaat accttgaggg catggcagac agggagaagc
    14521 aggcggaggt aaagtctgtt tctgatttac tggacgagat tgaggacgaa accctgtacc
    14581 ttgctttagt tacggtagac aggcgcacgt tacaaatcgt cctgctgaaa atgcagggct
    14641 attccactaa ggagattgcc ccgcttgtgc atttaacggc aggggctgtc tatgcgaggt
    14701 taaaccatct gcggaagaag ctgcggaaaa ttttataagc gtctcatata tcctgctttc
    14761 ctatgggcta tcgggtgggg agggaaaact ctctcctact ttttagcagg aggaaaggga
    14821 atggcatacg gggcaaagac atacaagctt cgggaggaat ccacggaaag cggcgcaagg
    14881 tattttatca gctttaagga cgggcagagg gaacatcacg aactggaagt gtctgaacaa
    14941 ttattcattg aatttcggca gatggaaagg aggaacagga accttcagca atggaaccag
    15001 cggcacaggg agtttaacga ggtgtgggac gaaacccttt acagacgtgc gttaagagtg
    15061 cctaaaacgc ttgatgaaag aatgattgaa aaagaacgaa atgaaatgtt ccttaaagcg
    15121 gttgcccggc tgccagagat acaaaggcgg cgtttcctgc tctattacga gtatgatttc
    15181 aatttttacc aaatcggcgc aatggagcat tgcacagctt ccgctgtcca gaaatctgtt
    15241 tcggttgcaa gggagaaagt taaggcggaa atgaggaagt atctccaatc ttaacgatta
    15301 ctgcccgaaa aaaaaattca tttttatcgg cgtggggatg gcggcattac atactctcac
    15361 ttctttgtgt gggagtaaat ctatttaagg aggtcacgcc tatgtgcaac aaaaccaaag
    15421 acactgcccg aaaggaggaa agccatgcag aagcttcaga cagtcaatgc cgacacgctc
    15481 ctctatgagc cgcttgagaa gccgtccttt gtggtggacg gtctgatacc cacgggctta
    15541 accctgttct gcggctcaca gaaaatcggc aagagctggc ttatgctaaa gctctgccta
    15601 tgcgtgtcgc agggaatccc cttgtgggat atgccgacac gggagggcga cgtgctttac
    15661 ctctgccttg aggacacgtt ctgccgcata caggacagat tgttccgctt gacggacgaa
    15721 gcaagcgggc ggctctactt tgcggtggcg agcgacaagc tgtcagacgg tcttatcgtg
    15781 cagttggagg attatctgaa agaatacccc gacagcaggc tcattgtgat tgacaccttg
    15841 cagaaagtcc gcaccgcatc caaagacaat gcctatgcaa gcgactacgg ggacatctcc
    15901 cttatcaaag actttgccga caggcactca ttagcggtca ttgtcgtaca ccacatccga
    15961 aagcagaacg acagcgacgt gttcaacaaa gtgtctggca cgacgggatt gacggggagt
    16021 gcggacgcaa cctttgttct ggaacaggaa agccgtgcgt ccaatgccgc aaagctgtat
    16081 gtgacgggca gggacacgcc ctatcaggag ttcacgctcc gcttccgtga ttgcagttgg
    16141 gaactggtgg aacggaaaga gcaggaacag cttgccaaag aagcgatacc agatgttctt
    16201 tttcggctgg tggattttat gcaggacaag gaggaatgga cgggaacagc aacggagctg
    16261 ttagccgcta tgggggaaac ggaaaccgtc cccacggtga ttacgaaatg gctcaatgaa
    16321 taccgcacca cgtttttaag cgaaaatcat atctgctacc agtacagccg caaaaaagac
    16381 ggcaggcaga tagccctcgc aaggcagacg ggtgacagcg gtgacagtga tgacagcggt
    16441 attgggatac cccctgttac tgtcattgac acctaaagcc atgtaaagct ggcggctctg
    16501 ccctgcgggt gacagcggtg acggtgatga cggcaatatt gggatacccc cgccgctgtc
    16561 atccctacgg gagaacaccc cgtaaacgca agatgcaggc gtgggcatta ctcgcccttt
    16621 tcggctcgta aagccacccc tgcggtcaga aaaatctacc gattttcccg actgcgttta
    16681 cagggtgtaa cacactacac tttgccctgc aaagagccgt gtgccagacg ttccctctgg
    16741 actcccttaa agcagggctt acgccctgcc catcctgcgc cttgcggctt cggatggaag
    16801 aatgtgcggt gacagtcggc ggctaagagg gggtatccca aaaacaccgt catcatcgtt
    16861 ataaccgtca cgcaccagag aaagacggca ggacggtgac agtgacagcc aagagggggg
    16921 tatcccaaaa tcactgtcat caccgtcacc actgtcaccc aaaggacagt tacccgccga
    16981 agaaaggagg aatcctgcca tgccctatgc aatcctgcgt ttccagaaac gcaaggcagg
    17041 cggcgttgcg gcttgcgaac gccacaacga gcggaagaaa gaagcctata aaagcaatcc
    17101 agatattgat atggaacgct ctaaaaacaa ttaccatctc atagcaccgc caaagtacac
    17161 ctataaaaaa gagataaacc gaatggtagc tgaagcagga tgcaggacga ggaaagacag
    17221 cgtgatgatg gtggaaacgc tcatcaccgc atcgccagaa tttatgaacc agttaccgcc
    17281 cgaagaacaa aaagagtatt tccagacggc tcttgacttt atttcggagc gtgtgggaaa
    17341 gaaaaacatc ctctccgcag tcgtccacat ggacgagagg acgccccata tgcacctatg
    17401 ctttgtgccg cttacgccag acaacaagct gtcagcgaaa tccattttag gcaaccaaaa
    17461 gagcctttcc gagtggcaga ccgcctacca tgagcggatg ttcgcaaggt ggagcgaatt
    17521 ggaaaggggg caatcctcaa tggaaacgaa gcggaaacac atccccacat ggctctataa
    17581 gttgggcggc aggatggata aacagtatga agaaatcgtg tctgccctct ccgacatcaa
    17641 cgcctttaac gcaggaaaga aaagggataa ggcgttagaa ctgctctccg catggcttcc
    17701 agacgtggag aaattctcaa aggaaatcag taaacagagt gcttatattg acagtttaaa
    17761 agagagaatc gggcaggaat ccgactatgc ggggcgtatg cgtgatgaga agtacgagca
    17821 ggaacgaaag gtacagaaag ccaaccagag gatatttgag ttgcagaaaa ccaatcagca
    17881 gatggaacgg ttattgaaaa agataccgcc agaagtgtta gaggaattgc agaaaagcaa
    17941 tcggaataga gcaagagaaa ggtaggtagc agattgaaga aacaggattt taaggtatta
    18001 aagaccgcag atttataccc gtttcccgac aacccgtttc atgtggtgga ggatgaaatg
    18061 ctgtcagagt tggcggaaag cattaaggag tttggcattg taacgccgat aatcacacgc
    18121 ccgaaagagg acgggaatgg atatgaaatc attgcgggac agcggcgtgt ccgtgcttcc
    18181 gagcttgcag ggataaacac aatccccgct tttgttctgc ccttagaccg tgaccgagcc
    18241 atcatcacac ttgtagacag caatctccaa agagaaaata tcctgccatc ggagcgggca
    18301 tttgcctaca agatgaaatc cgaagccatg aagcggcagg gtttccgaac agatttaacc
    18361 tcgtcacaag ttgggacgaa gttgcggacg gacgataaag tggcacaggg cttcggcgtt
    18421 ggcaggatga ccgtgcagag atttatccga ttaacggaac tgataccgcc tattttgcag
    18481 atggtggacg aggggaaaat cgcccttacg cctgcggtgg agctgtcctt tttgaagaaa
    18541 ggcgagcagg aaaacctatt cgccacaatg gagagcgagg aagcgacgcc ctcactctca
    18601 caggcacagc ggatgaaaag cctaagccag agcaggcggc ttaacatgga cgctatcttt
    18661 gcgattatga cggaggaaaa gggaaaccag aaagaaaccg tgaaaatcgg catggagcgg
    18721 ttaaagaaat acttcccgaa aggaacaacg ccgaagcaga tggaggacac catcatcaaa
    18781 cttttggagc gtgaattgca gaggaaacgg ggcagggaca gccgctaatc ttctcttttc
    18841 ggagggtaaa tacaaggaac tggacgtaag aaaggtcaag gcaataagac cagaaagggg
    18901 caggaaatga aagaaatcca gtatgagatt gtaaaggaaa ttgccgtgtt gtctacgagc
    18961 gaaagcggct acacaaagga aatcaattta atctcatgga acgggaaaga gccgaaatat
    19021 gacatccgca gcttttcccc caaccgtgag aagtgcggaa agggaatcac gttgaccgct
    19081 gatgaagcgg cggctctcct taaagcattg caggaagaat tgaacagcgg ggattgattg
    19141 tgtctgattg gcagggcggg agcattttta tatactcccc tgccctgtct ggaaaggaag
    19201 aattgagtat gggcgaggat aagaaagcga acaaaaagag aaagcgcatt gtggcaagac
    19261 agccagtgca gatgattgtc agccgtgaat atgtcggcac acagaccgtt gccgaagcgt
    19321 tcatcccgat tatctccgag gacatccgca ggaagattgc cgaaggtgac accttcgaca
    19381 atgaggatat atccgcttag aatgtacgca atggaacatg aaaacagtta gggcagatac
    19441 ggaggtttta cagtatggca ggaatcaaag aagaaaagaa aatctattca gtcggcattt
    19501 actgccgctt atctaaggat gatggcacgg aaaacgagag tgcgagcatt gccacacaga
    19561 aatccatcct cacggattat gtgaaaaagc agggctggca tctggcaaaa acgtatgtgg
    19621 acgacggtta ctctggtaca aatttccaaa gaccgagttt ccagaacatg attaaggaca
    19681 ttgaaaacgg gctgataaac tgcgtgatta caaaggattt atcccgtctg gggaggaatt
    19741 atcttgactg cggactatat ctggaagtct ttttcccaga aaataatgtg cggtatatag
    19801 cggtcaatga tggcgttgac accttaaata aatcggcgat ggacatcacg ccgttccgca
    19861 acatcctaaa cgaaatgtat tcagccgatg tgtcggtcaa gataaaatcg gcatatcgag
    19921 cgaggtttca gcagggaaaa ttcatgggga catatgcacc ctatggctat atcaaagacc
    19981 ccgccgacca caaccatttg ctgatagatg ataaggtggc gcacgttgta agggagatat
    20041 tcgaccttgc attagcggga aatggacttg caaaaatccg caagcacatc aacagtcagc
    20101 atattttacg ccctgccgct tatgcggcgg agcagggggc ggcaggctat gaacggtact
    20161 ttgagggtaa tgaggagaac cgctatattt ggagcgagaa cagcgtgagg ggcattttga
    20221 gaagcccaat atatgcggga aaccttgcag gctacaagcg gattgccgcc aacatgaaaa
    20281 gcaagaaacg cccctctaag ctgcccgaag aatgggaagt gatacccgac acccatgagg
    20341 ggatagtcac gcaggaggaa tttgatattg tccaacagct tatgacgagc cgcagacggg
    20401 aaaagaataa gggcggcttt gagaatattt tttcaggcgt tatcaaatgt gcggactgcg
    20461 gctatgctct gcgggctatg agtgcaaaca ggaggaaacg ccccgacatc atcgactgca
    20521 tacaatacac ctgtaataat tatggcaggt acggcaacgt catgtgtacc gcacacgcca
    20581 ttgaagcgag ggacttattc aacgccgtcc ttgcagacat caaccgtttt gcggatatgg
    20641 cggtgaatga cgagcgggcg gtaagggcga ttgagaagcg gctcacggaa acagaccaga
    20701 gcaaggcaaa ggcgatggag aaagaacgaa agaagctgaa taaacgcctt gcggagcttg
    20761 acaggctgtt ttcctctctt tatgaggata aggtcatgga gcgtatcacc gagcggaatt
    20821 tcgagatgat gtcggggaaa taccagaaag aacagcttga aatcgaagcg agattaaaag
    20881 aggtaacgga aactcttaac gaaagctatg agaaatcgca gggaatccgt gacttcctct
    20941 ccttaatccg caactatcaa ggcttaaagg aactggacgc aacggttgta aatgcactga
    21001 tagacaagat acttgtttcg gagcgtgaga aatcagcgga cggaacagtg aaacaggaaa
    21061 tcaagattta ttataaattc atcggctttg tcggtgaatt acatatcaca cctacaaaac
    21121 ggtggacagc gttacccgct aaacactgta cggtgtgcgg cattgaatac gtccccggct
    21181 ctggcatatc aaagtattgc cctgcttgtg ccaagaagat acagagggag aagtcaaacg
    21241 agagcaaacg caggagcagg gagcagaaaa ggatggcatg tattgaactg tccgcaaaaa
    21301 atgaccgact gatatggtgc agaaagaggg atacatacct ttcacttcat agagaaaggg
    21361 ggtatgattt tgaaaccatc agacttccaa aagacgatac agtgtcagtt ggactgtaag
    21421 ctcaaaaagg ttgtgaaagg cagtgtccgt aactaccgca aggaattagc cagatgacag
    21481 gcaaaggaag tacccttttg tgagcttcca gaaattgtta ttgagaaatt gattgtctgg
    21541 gatgattacg aaagtgacta tgcgacattc gatgtgtgca gtacggaaat ccgtgtgctt
    21601 gatgaagaac ttgaaaaata caggatatat cggcatttac caacttatga aaagataatc
    21661 agaaatttag tgtattttta taataaaaat ataatgtttg tatacaaaaa tattaaagac
    21721 attttagtaa gttttgtata taagcaaaca tgtatttttt aatatagtta tctaaatatt
    21781 attttatata caataaaata ggactcctaa aaataaaaaa tatatcataa atataaataa
    21841 gtagatatag gttttaattt gatttataaa cctattataa aataaaattc atgattttta
    21901 taagcaataa aatttttagg aggatatatg atgaataaaa gaatgaaact aattccatat
    21961 gaaataaatg aaaatctaag aggtgcaaaa aataaattcc catatggaat aaaacaaatg
    22021 aatgctaggg gaatgtggga tgaaggttat actggtaaaa atattgtagt tggtataata
    22081 gatacaggtt gtgatatatc tcatcctctt ttaaaaggaa aaataattgg tggtgcaaat
    22141 tttagtgatg acagtaatgg aaataaaaat atatatgagg attttaatgg tcatggaact
    22201 catgtggcgg gtattatagc tgcatctaat tataataatg aagttatggg agtagctcca
    22261 gattgtaaat tattaatagc aaaagcatta aataaagatg gtaccggaac atatcaaagt
    22321 ataattaatg ctattaactt tgctgtaaat aacaaggttg atattatatc tatgtctctt
    22381 gggggaaaca aagatgataa gaatttaaaa aatgctgtca tgcaagcagt aaaaaataat
    22441 atttctgtag tgtgtgcagc aggtaataat ggagatggtg attctagtac aagtgagtat
    22501 agttatccag ccagttatgc tgaggtaata gaagtaggtg caataaatga aaactatttg
    22561 gttgaaaagt ttagtaattc aaatactaca atagatttgg tggctccagg aagaaatatt
    22621 atatcgactt atatggataa taaacttgct attatgagtg gtactagtat gagtgcacca
    22681 tacgtatcag gctcattagc actaattaaa gaatgggcaa gagaggagtt tgaaagagat
    22741 ttagatgaag ctgaactgta tgcacaatta ataaaatgta cgagagcgct tggaatacct
    22801 agaacggaac aaggaaatgg atacttatat ttaaatcttt ataaatacaa gaataatagc
    22861 aaaagataat ttttgatttg atgataaaat agctatatta tatagagtcg agacaaataa
    22921 taaaaattac ttaggtgtaa gattttacat caatgtataa aggtattaag tgataaaatt
    22981 tataaacata ttagctagtt agaattgaaa aataaatata cgatattata gatagcacat
    23041 ctggaaaagg tgtgtttttc tatgtactta tacatataaa agatttataa gagatgcaaa
    23101 agtactataa gttagaaatt tttctatgaa agattagaaa tacgatacag tgttttgata
    23161 ttataaaatg aatataaagt ataaatgata aatgttatca ataagtattg aatttcaaaa
    23221 tcaattgtga tattctatat ttagaaaaat aaagagattt cagataaaga aatcttttgt
    23281 tttttagatg ttagttagct ataactaact attgattaat taaaaaatac tacaggaggt
    23341 aaaaatgagt aaaatatata ttgtttattg gagtggaaca ggaaatactg aaaaaatggc
    23401 aaattttgtg gctgaaggtg tgaagttaaa aggtaagaca ccagaagttt tagatgtgag
    23461 cttactgaaa ccaagtgatt taaaagaaga agataaattt gcattaggtt gcccatctat
    23521 gggagcagag caactagaag agggggatat ggagccattt gtttcagaat tagaatctat
    23581 ggtatcaggt aaacagattg gattatttgg ttcatatgga tggggaaatt gtgaatggat
    23641 gagagattgg gaagaacgta tgcaaaatgc tggtgctaca attattggtg gagaaggaat
    23701 tacagcaatg gaagacccaa atgaagaagc aaaagatgag tgtatagaat taggcaaaac
    23761 gttagctgaa taaatttgta tattataaaa atagtataaa tagcaactaa tgatgatagc
    23821 agtatataat aataagagag actaatatta tgatacataa gaaaatatcc ttaatagaga
    23881 tagataaaat attagtctct tttaatataa aattagattt ataatttaat gttgtttttg
    23941 aattggtaca atctcattat aaaaagataa taattgattg ataacataag tgataaaggt
    24001 agtatcttgt gccaaacaaa gcatatttgc tccaatatta acccattctt caatactttt
    24061 cttttctatt gtaggaaaaa taataaaggg catcttatat tttttgcata tttcagcagc
    24121 atgagaaatc atatctttaa aacgccgcca ttggagacaa tgagcggcgt tttggtgtaa
    24181 tggctaagga gaggagggat aggaatgaat atccgagggg caaacagggc aggtgtggcc
    24241 atacctgcct tgcttacaga tataatcaaa gccggtaaag attgcaaatt taccaagggt
    24301 tccatctagg ggaaagtcgc attagtctaa ttaatacctg tctaaccacc aaaccttatt
    24361 gttttgccat aaacataatg ttgcttgaca gaagcaggaa tagcttatat aataaaaacc
    24421 aacagtcggt ttttaataag aaggagttat ataatgacta gaaagaacaa tccaaagcaa
    24481 gctattgaaa atatcattac catttctgca aagttgtttg ctgaaaaagg atacgacaaa
    24541 acaagtatgc aaaatattgt tgatgcttcg ggaatgtcaa agggcggaat ttttcatcac
    24601 ttcagttcaa aagaagatat ttttaatgtg gtcatggaaa ggcgctttga acagataata
    24661 gagacagtaa atcaatggct cggtgaaatg catggactta cagcaaaaga gaagcttaga
    24721 agtttagtta ggcggcattt aacggatgag gtgataaaag aatctagtaa catgattaca
    24781 agcgcgatag aaagtccgca tatcatactg gcatttacac aggataattt gaaaaaacta
    24841 gcaccaatat tagcaaatgt tttacgagaa ggcatagagg atagaagcat catcacagag
    24901 ttcccagatg aatgcgcaga ggtaattttg ctgctcttta acttttggtg tgatacagat
    24961 gtatttcagg gggactttcc tacaattcgt aaacggtttc aattcttaca gtttcttatg
    25021 agacaaatag gagttgatat cctggaagat gagattatag aatcaattaa tattttttat
    25081 gaaaggaaac caatgggaca ggtagagtaa caaaactaaa atagcaacat acttttttta
    25141 atgttagagg agatatctat ggctaaaaaa attcatgaaa gaaggtactt aaaatgatat
    25201 taatcacact ttgcatacta gttgctgttt ttgttctggc gtttgccggt ttgcgtctga
    25261 tagcccccac gattatacgt acaagccata aaatagaagc tcctggaatc gaccgcatgg
    25321 aaatggtgga gatcggcggc atacagcagg cgttatatat ccggggacaa aacgcagaca
    25381 accccatcat cctttgggtt catgggggtc ctggtaattc aatgatgcca tttttacatc
    25441 tgtaccagta tgagtgggaa aatgatttta ctgtggtgaa ctgggatcag cgaaacgtag
    25501 gcaaaacata ctttgcgaac gatccggcag cagttttgca gaccatgagc gcggagcgtg
    25561 ttctgcaaga cgtccatgag gtgacagcat atttgaagca acaattcaac aaggagaaaa
    25621 ttatccttat aggtcatagc tggggaacgg tgctgggaac tatggctgtg caaacctatc
    25681 cggaggatta cagcgcgtac attgggattg cgcagaccgt taatatggtg gacaatgagc
    25741 gcgtgggcta tgaaaagaca cttgaaatgg tgaaagctgc aggaaagcaa aaagatatca
    25801 aggcgctgga agctgttgca ccatatccgc cagcaaaata tggtccaagc tatttggcgg
    25861 ctcttatgac agtaagaaca taccaaagca agtatgggct ttctataaat atggatttgg
    25921 gcaatataat cagtatactg gcaaccccgt attattcatt gagcgagtta aaaaatacaa
    25981 ccattgtcaa tccaaatgcg attgacaatc aaggtgatgt attgcggttt ttagtggaag
    26041 aagctgacat aagaaattat ggagttgact atcaggttcc tgtatactac atcatgggag
    26101 aaaatgacta taatagcccc tatcttccag cgaagaaatt ttttgatgaa atagtggcgc
    26161 cagacaaaca gatttttttt ataccagatg cagaccatct gccatttctt agcaaaaagg
    26221 cagaatttga ctgcatattg ttagaggaaa tcgctccaaa gttagcaaag taacaactag
    26281 gacagtaacg tggatatggt ggcaatttct tttactattc cagaacgctt cagtttgttt
    26341 ccagcaactg gtttcaatga ggtgcttgag atatatctat ttagagggtt ttaagagagg
    26401 actatcactt atggacattt tgtccataag tgacggggtc agcaatcgga aagggtggtc
    26461 atgctatgag tgaactgaga cctttatatc agacagttaa aaaccatgga gtgattatga
    26521 aaccataaga tgattcttgt aatgtacagg agtcatcttt tatatttatt ttctttttta
    26581 gatttaattc tctacttgat ttagatacaa gagaggcttc gccttgtttt aagtatattt
    26641 ttctacttat accattattt cttttttatt tgcttttcca ctttaaataa tacaacttca
    26701 acacaatttg aagctaatat cttttattga ctattgtata aagtattttt ataatgttct
    26761 tattcatata aaattttatt ccacttacta ttgaatttta ttaagaaaaa gatttattac
    26821 tttctatatg atagaaaaat aagtggtatt agttatttct aggaagagaa tatagagtat
    26881 cctatattta ttacaagatt aaaaaaagca ggtttatata ttgaagatat aagaatgctt
    26941 tcatttttgt gatataagaa acaatatgtt tgtgatataa gagacaatat gatagataaa
    27001 agaatttaat tatttcagca atataagcaa gatattaaaa taaaatcatg aactagaaga
    27061 gtgtatatac aaaatagctg agaagataga ctatttaagc aaaaaaggta tgtaaaaaat
    27121 aatttttcta aaagaatcca acagtttttt atttctataa gaaaaataat attaattgga
    27181 acaatttaat aatattcttt cttagatgta tttctatata gtattggaca atgttatata
    27241 cagttattta tatatgcaat aaaaaggaaa attatgataa catatattga tgtaagcagt
    27301 aaaaatactt tcatttatag atataattat tttgggaaaa cactgatttt acaaaataaa
    27361 aaagtaatta tagaatttat caatatatta aaaaactgaa ttg
//
LOCUS       DAGSWI010000047        27373 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.047, whole genome shotgun sequence.
ACCESSION   DAGSWI010000047 DAGSWI010000000
VERSION     DAGSWI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.047"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            118..1722
                     /locus_tag="MJF80_001819"
     CDS             118..1722
                     /locus_tag="MJF80_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0280482.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDKNPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2538..3809
                     /gene="serS"
                     /locus_tag="MJF80_001820"
     CDS             2538..3809
                     /gene="serS"
                     /locus_tag="MJF80_001820"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBZ0280483.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            4133..5638
                     /locus_tag="MJF80_001821"
     CDS             4133..5638
                     /locus_tag="MJF80_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBZ0280484.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            5752..6450
                     /locus_tag="MJF80_001822"
     CDS             5752..6450
                     /locus_tag="MJF80_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBZ0280485.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            6735..7823
                     /locus_tag="MJF80_001823"
     CDS             6735..7823
                     /locus_tag="MJF80_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280486.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            8062..9252
                     /locus_tag="MJF80_001824"
     CDS             8062..9252
                     /locus_tag="MJF80_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0280487.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            9343..10845
                     /locus_tag="MJF80_001825"
     CDS             9343..10845
                     /locus_tag="MJF80_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBZ0280488.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            10917..12173
                     /locus_tag="MJF80_001826"
     CDS             10917..12173
                     /locus_tag="MJF80_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBZ0280489.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGIRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            12345..15119
                     /locus_tag="MJF80_001827"
     CDS             12345..15119
                     /locus_tag="MJF80_001827"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBZ0280490.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(15184..15369)
                     /locus_tag="MJF80_001828"
     CDS             complement(15184..15369)
                     /locus_tag="MJF80_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBZ0280491.1"
                     /translation="MFFISDIYIKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            15744..16922
                     /locus_tag="MJF80_001829"
     CDS             15744..16922
                     /locus_tag="MJF80_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBZ0280492.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      17049..17134
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            17252..20170
                     /locus_tag="MJF80_001830"
     CDS             17252..20170
                     /locus_tag="MJF80_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBZ0280493.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIQEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGILKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYEVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSVLEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDANFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSVKNPGTYYLVEIMAPRGY
                     EKLKDKVKVEIGNEDIGKIVDIGNIENIRQENPPVNPPAPPNTDEPMVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPVPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNRVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      20355..20440
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            20540..21202
                     /gene="zmp1"
                     /locus_tag="MJF80_001831"
     CDS             20540..21202
                     /gene="zmp1"
                     /locus_tag="MJF80_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBZ0280494.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(21321..22283)
                     /locus_tag="MJF80_001832"
     CDS             complement(21321..22283)
                     /locus_tag="MJF80_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBZ0280495.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(22453..23643)
                     /locus_tag="MJF80_001833"
     CDS             complement(22453..23643)
                     /locus_tag="MJF80_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBZ0280496.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            23949..24395
                     /locus_tag="MJF80_001834"
     CDS             23949..24395
                     /locus_tag="MJF80_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBZ0280497.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            24397..25491
                     /locus_tag="MJF80_001835"
     CDS             24397..25491
                     /locus_tag="MJF80_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBZ0280498.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMKMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGGTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLFSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            25579..26109
                     /locus_tag="MJF80_001836"
     CDS             25579..26109
                     /locus_tag="MJF80_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBZ0280499.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            26437..27270
                     /locus_tag="MJF80_001837"
     CDS             26437..27270
                     /locus_tag="MJF80_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0280500.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENV
                     ENIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGGKITWGIITIACLLIA
                     ITVFPKLPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDILVFIALLIIGVYKLFKKY
                     N"
BASE COUNT        10921 a         3076 c         4646 g         8730 t
ORIGIN      
        1 aaaaataaaa cttttatatg attaaactct aaagaaaaag tttataaaga agataatata
       61 attaagcaga aaataatgaa aaaaatttat taaagtagac aacgtaagga gaattttatg
      121 aaagcaaaaa tttattacaa tggaaatatc atcacaatgg aagattcaat atgtggagat
      181 gctattctta taaaagacaa aataataaaa aagataggaa caaaagaaga agtatttgct
      241 ttgaaaaata aagatacaga aattatagat ttacaaggta agacattgat gccatctttt
      301 atagattcac atagtcactt gatagctttt gctactactc taaagctagt tcctttagaa
      361 gatgcaacca gttttaaaga catagtaaaa aaaatacagg atttcaaaga gtcgaataat
      421 ataaaaaaag gagactggat aattggattt tcatatgata ataatttttt agaagaaaat
      481 aagcatccag ataagagtgt actcgatagt gcatcttcag aaaatccaat actaatttca
      541 catgcttctg gtcatatggg agttgcaaat acattggggt taaaacagct tggtgttacc
      601 aatgaaacac gtgacccaga aggtggacat ataggtagag tagaaggcag tgaagaacca
      661 aatggatatt tagaagaaaa tgcatttttt aatgtagcat ctaaaataaa acaaccatca
      721 tcaaatgaga tttttaattc aattgaaaaa gcacagaaca tatatctaag ctatggtatt
      781 acaacagctc aagaaggttt aatggaagaa aatcaattta acattttaaa agcaatggca
      841 aatcaaaata aattaaaaat ggatgttgta ggatatgtaa atttgaaaaa aagtaagtct
      901 gtagcagata ataataggga gtttataaaa aaatatataa atagatttaa aataggaggt
      961 tataagattt tcttagatgg ttctccacaa ggtaagacag catggttgag tagaccatat
     1021 gaaaattcag atgatggtta ttgtggatat cctatataca aagatgaaga agtagaaaag
     1081 tttatagata tttcactaaa agaaaagatg caactcttaa ctcattgtaa tggagatgca
     1141 gcagcagacc agttgataga tgcgtttgag aaagttttaa acttaaaaga gcaaagtagt
     1201 gaaaataata taagaccagt tatgatacat gctcaaacag taagagcaga ccagattgat
     1261 gatatgaaag taataaatat gataccttca tactttgtag ctcacacata ttattggggt
     1321 gatattcata tcaaaaacct tggagaagat agagcgttta agattagccc acttaaaaca
     1381 actatagaaa agggattaat ttacactctt catcaggata caccagtaat agcacctaat
     1441 atgctggaaa cagtatggtg tgctgtcaat agaattacaa aaaaaggtat acaaatagga
     1501 gaaaatgaaa agataagccc attagatgct ttaaagggtg ttactataaa tgcagcatat
     1561 caatattttg aagaagataa aaaaggttca attaaagaag ggaaattagc taatttgata
     1621 atattagata aaaacccact tacaatcgac cctatgaaaa taaaagatat taaagtattg
     1681 cagactataa gagaagggga agtattgtat tctttgaagt agatgaaagt tagatatttt
     1741 ataatctata aaaaatagtt ttatataata agttaggtag aattaaataa gattacagtt
     1801 aaatcaacaa ttatattttt attaaatttt aattattgta ttgaagattt ataagtataa
     1861 ttttattgat tctacctttt taaataactg tcgatttatt aaagttatct atgtactata
     1921 ttaaacaatt aagtagttat taattgaact tattagttta ttaaaaaact atgtatatta
     1981 ctttgcagta agactttgga actaaatcta taatagcaaa tttaaattta ttaaatagat
     2041 taccttataa catttcatat aaaatatatt gtgaatctca gaaaattact atttttcttt
     2101 gagtattttt tgtaagcagt tctaaaagat tcaatttcat acctattact tgaatcattc
     2161 atcaaaataa ttctaattta aaaaataatt ctatccaaat aatagatata taggactatg
     2221 ttataatata aaaaaattta tataaaagtt aataactata caagattgat aaagaaaaag
     2281 tcaaaataga taattgatta gcaaaataga tataattata atgaagttaa atctagcaat
     2341 attctcctaa aattaaattt catttattta attaaatata atttgacaat gggcaaaaaa
     2401 gtgagtaaaa tataaataaa ataaaaataa caggttcgga taaagaaaaa ggtttcttaa
     2461 cctcaagcat aaagtatatt atctaagtta tttgagatta agagataaat ccaatcttag
     2521 gtactgagga ggaaaaaatg cttgatataa aaagaataag agaaaattta gattgtataa
     2581 aaaaagcaat ggaaagaagg ggcgaaaaag actttaacct tgatgaagta gttaagttag
     2641 atgatgaaag aagaaagatt cttcaagaag ttgaagttat gaaaaatgaa ttaaatacag
     2701 catctaaaaa tataccaaat cttataaaag aaggtaaaga tgtagaaaat gaaaagatta
     2761 aactaaaaga gttatctgat aaaattaagg taatagacca aaatcttaaa gaagtagaag
     2821 ataagatgga atacctatta atgagaatac caaatgtacc acatccagaa gtaccacaag
     2881 gtgaaactga tgaagataat gttgaagtaa gaacttgggg aaagactact acctttgatt
     2941 ttgaatctaa ggctcattgg gaaattggaa cagaattagg tatacttgat tttgaaacag
     3001 ctagtaaaat aactggttca agatttactc tttacaaagg attaggtgct agattagaga
     3061 gagcattatt aaatttttac ttagacacga atacaaaagt aaatggatat acagaagtta
     3121 ttccaccatt tatggcaaat agaaatagtt ttttaggtac aggacagtta cctaagtttg
     3181 aagaagatat gtttaaaata gaaggtctgg actattttat gattccaaca tcagaagttc
     3241 ctttaactaa tatacatgct aatgaaatac ttaaatttga acaattaccg ataaattata
     3301 cagcttatac tccatgtttt aggtcagaag ctggttctgc tggaagagat acaagaggtt
     3361 tagtaagaca gcatcagttt aacaaagtag aaatggttaa aatagttgca ccagaagaat
     3421 cttacaatga gttagaaaag cttactaaca atgcggaaac tatgctacaa ttattgaatc
     3481 tgccttatag ggtagtaaag atatgtacag gtgatttagg atttacggct tcttttaaat
     3541 atgatgtaga agtttggatg ccaagttaca ataggtatgt tgagatttca tcttgttcaa
     3601 actgcgaaga tttccaagct agaagagcag gaataagatt taaaagagat aaagattcta
     3661 aagctgaata tgtacacaca ttaaatggct ctgggttagc tattggaaga agtgttgcag
     3721 ctatattaga aaattatcaa caagaagatg gttctgtggt tgttcctgaa gttttaagac
     3781 catatatggg agtttctgtt attaaataag agttaaatta tgtattttta agcaatacaa
     3841 taatatagag gtaataaaaa ataattaata taaaagtgat acaataatat agaagtaata
     3901 aaaaaatagt taatataaaa gtgatacaat aatatagaag taataaaaaa atagttaata
     3961 taaaagtgat acaataataa tagaagtaat aaaaaaaaat aattaatata aaaatgtgta
     4021 caaactaatg aatattgata tagtaagcta tcatatgaaa gagtatgtac acattttttt
     4081 tactaatttt tcacaatatg aacataaata atttacagga aaggaatctt ttatgaaaat
     4141 tcaaaatact gagtggggat atattgaatg gaaacatact tatgatgaaa ataatccaaa
     4201 acaagctatg aatatataca ttgcagtaac tatgcctggt aagaaacatt ttaatcatgt
     4261 tcattatggt caagagcaga tgatatatat tttagaggga gagggtttgt atattataaa
     4321 tggagtttgg aagccatttt atcaaggtat gattttttat atagaatctg gtagcacaca
     4381 tgaaactata aatacaggcg atagagaaat aaaagaactt attgtttcaa ataatgtaga
     4441 tgatgtagga gagagtgaag ttatagatat taatcctaat aattatttaa agaagacctt
     4501 gataaattat agtgaaagta cattaaattt gtatgcagca gtagaatcaa ttcgtggtca
     4561 atttatagac ccgtttaaaa ttccattaat tatatatgat gattcatgga atatagtttt
     4621 aaaaaatcct tattttccac ttttttgttt tgaaaaatgt aatcctatga aatttcctca
     4681 aaattgtgat tgtatgaatc aaaaaagtag caatcaattt gtatgtgaat atgggataac
     4741 tatatataat ataccaatac tgtacaaaag caatagcata ggtgttataa gaggagggta
     4801 tgtactatta tctgatttga atttagatac agagcataat aatttatatg atattccaga
     4861 gggagctgct agaagtataa aacgcctctt gaaacaaata tctaaaaata taataaattt
     4921 ttgctctttt aatgatataa gaaaggattt acaagaaaaa gagaagacta tagcaagaac
     4981 atatcattat ggagagcaac ttgaaatgaa tttaaaagta gctcaagata tggttactaa
     5041 tttaagaata aatcatcact ttttatttaa tacattaaat tcaatggcaa gcatagcact
     5101 agatgatggt agttatgatt tgtatagtgc aataatagat ttatcaagaa tgtttagata
     5161 tacaatgagg tctgatttaa gatttgtaga attagaatct gaaatattat acattaaaaa
     5221 ttatttgaat ttacagaaat tacgttatgg agatgcactt aaagtaaaat atcttattcc
     5281 agagaagtta tataaccttt ctgtgccttt taactttatt caacctattg tagagaatgc
     5341 atttacacat ggatttagag atattgacac agaaaagcga attgaaataa ttgcaagatt
     5401 agattctcag tatgccatta tagagattca taataatgga actatcttag atggaaataa
     5461 tatagacaaa ataaaggcag gtattcgtag taataatggt catggtttgt ctttaatata
     5521 tacaaaattc acatctgctt atggaaataa ttttgatatg gatataaagt cgagtgataa
     5581 tgaaggaact tacataatga ttaaaattcc tattgaaaat tataaggagt atgtatagaa
     5641 cttgtgaaaa attaataagt tttataaatg ttaagaaata tattagatgt ttttatgttg
     5701 ttttatatat cttacttttt tatattatta agctaaaagg gaggtattct catgagaaga
     5761 gcactaataa tagatgatga agaagctgtt ctaaaaataa taacacattt tatagaaatg
     5821 aaagatatgc caataaaaat tgttggtaaa gcaacatctg gtgatgaagc agtagataaa
     5881 atcattggtt tagagccaga catagttttt atagatattc aaatgccaat atacaacggg
     5941 ttagaagtaa tagaaaaaac atctcattta tctaaaaaaa taaactttgt agtaataact
     6001 gcatttaact attttgaata tgctcaaaaa gctttgagat taaatgtaaa agatatactc
     6061 cttaaaccat tggatatgaa agaatttact aagtctgtag aaaaaattat tggatatcaa
     6121 tataccaata atgatttatt aaatgagata ctggaatata taaatctaaa ctatagcaat
     6181 gacttaaagt taaatgattg tgctagatta tttttaacca atccatctaa tatatctagg
     6241 atttttaaaa gtaatttaaa tacaactttt atatcatatc taaatcatat aagaatagaa
     6301 aaatctattg agttacttga aaatactaca aaaagtataa atgagattgc agaaatagtt
     6361 ggatataata gcctaaataa cttttataaa aactttaaga tagaaaaagg aatgactcca
     6421 aaagtttata aactgaacac taaaaattga aattgataaa taaaaagtca atatagataa
     6481 ttgtttgagt gtgtctgaat aagtatactt taattatgat aaaaattaaa gcgctttaaa
     6541 catatttaat aatattaaac cttataaaag ctgtacctat ttatataaaa aaatatatat
     6601 aaataaaaag aaaaagatat acaaaataaa aaaagtgaat taaatactgt ttcaaaaaga
     6661 cagaattttc atttaattta ctgagagttt atatttctta aaggaaggta actttataat
     6721 ggaggtaatt taatatgtca gacaaagtga atatgaaaaa tattatctgt attgcaggcg
     6781 cttatctagc aacagcaatt ggttctgggt ttgctactgg tcaagaaatt ctacaatttt
     6841 ttgcctcaca agggtatatg ggtttaattg gagcattaat atcaatggta ttgttttcat
     6901 ttcttggtgc tgaagttata actagaggta gagaattaaa acttaaagaa ccaacaagaa
     6961 tttacacttt ttattgtggg aagattttag gaaaatttta cgaatggttt agtccaatat
     7021 ttttatttgg gattcttata gtaatgattt caggtgcagg ctcaacttta actgaatact
     7081 atggtataaa tccttactta ggaagagtat tgatggctgt tgctacttta attacagtgt
     7141 ctatgggttt agaaaaatta gtaagcatac ttggacgtat tggaccagtg atagcagtat
     7201 ttacaattgc tgttggatta ataagcttgt ttaagcattt tgatggttta tctatggttg
     7261 gagaagctat gaaaaatatt gaagttacaa aagcatcaga aagtccaatc gtttctggtg
     7321 taatatataa tacattaaat attattgtaa tgattgtttt tttaacagga atgggagctt
     7381 ctattgaaag caaaaaggat gctttttggg ggggtttatt aggtggaata gtgtttatga
     7441 ctgctggtat agttatgtat ttggctatga tttcagatat aggaaatttg tatatgaaag
     7501 aaataccatc actttattta gcagataata tatctccgat tgtaggtgta tgtttttcag
     7561 ttgcattaat attaggtatc tatacaacag ctgttccact tttatggtct gtaaccaata
     7621 gaattgttga agatgaacat cctaaattta aattggtaac aacagtaata gctattttgg
     7681 cttgtatagg aggtttttta ccgtttggta aattagtaaa cattttatat ccatatacag
     7741 gctatatggg gcttttaatt ttagtctgta tggtgtataa agcaattaca tgtagaaaaa
     7801 aatcaacaca aagtcaggaa tagagccact tattaatagt ttttatttaa agaaattttt
     7861 tatataatcc gaatttaaaa agtcaaaata gttaaataaa ttgtaaaaca tgataatata
     7921 tttaattaaa ataatatagt aaaatataaa tataaactaa aattaaattt ataaaaaata
     7981 tctttataaa gattattaac ttagataatt tgtagttaaa ttataaaaaa taaaaaaata
     8041 aaattttgga gaggtgttag tatgcatgaa cttattttaa tctcagaaaa gtataaagaa
     8101 gatattttag atttttttac agatattcat atgcaccccg aattaagttt taaagaattt
     8161 aggactacaa aagctataaa agatttatta gtaagtttag acatagaaat attagattta
     8221 ggaatggaaa caggagttgt tggattatta aaagggaagt atgatggtcc cacagtagca
     8281 ctaagaggag atattgatgc attgccaata tatgaagagg ttgatattaa gtataaatcc
     8341 agatatgatg gtataatgca tgcatgtggg catgatatac acacgtcttg ccttgtagga
     8401 tgtgcatatg tattgtctca tattagagat agtcttcatg gaaatgtaaa gtttatattt
     8461 caaccagccg aagaagtcaa taaaggtgcg aagatgctag tggaacgagg agtaatggaa
     8521 aatccaaaag ttgatgctat atttggactt cataatcatc cagatatacc ttgtggaaaa
     8581 ataggtgtta agttaggtgg gttaatggca gcagtagaca ctattaagat agaagtaaat
     8641 ggatttggag gtcacggagg tataccaaac agaacaatag accctatagt agcatcaagt
     8701 gcaattataa tgggtattca aaccatagta agcagaaaca taagtccatt agaatcagct
     8761 gttatatcaa taggtactat aaatggaggg actgcaaata atgtaatttc agaaaaggta
     8821 gatatgacag gcacatgtag aagctttagt aatgaagtac gaaagaaaat atcagaaaat
     8881 ttagaaaata tagtttgtga gatagctcgt ggatatcaag caactgcaaa gctagattat
     8941 ttatttgatt taccagctgt tataaatagt aaagagatgt acgcaatagc ttgtaagtct
     9001 gtgtgtgatt tgtattcaga agatgctata gttgatccaa taccttctac aggaggagaa
     9061 gatttttcta tattcatgga aaaagcccca ggattttttt actggttagg ggtaggcaac
     9121 aaagaacaag attgtatata tcagtggcat aatcctaaat ttaaagctga taaaaattct
     9181 atattagtag gtacaaatgt actatgtcag tctgttataa attatatgga taaattaaaa
     9241 aataaaattt aagatagagc attctggaga taaagtttat gagggggtat tagtttgagg
     9301 aaaattaatt cacaggaaaa aaacaaagat agtggattat tgatgagggc actaaatgtt
     9361 gtagagagag caggaaataa acttccagac ccagtaacca tatttttatt actatgtata
     9421 attgttgtaa tattgtcagc agttatatct aatttaggag tagaagaaat acatccttct
     9481 acaaaagaag ttgtaaaggt agtaaatcta ctagagaaag agcagattca atcatattta
     9541 ggtagcatag ttacaaactt tcagtcattt gctcctttag gattagtact ggttacaatg
     9601 cttggagcag gtgttgcaga gaaatctgga tttatggaag tgttaatgaa aaagggtata
     9661 agtaaagttc cacaaaaact tgtaactgta gcaatcgtat ttgcaggtat gttatcacat
     9721 acagcagcag atgtaggatt tataatatta ccaccacttg cagcacttgt gtttttagga
     9781 attggaagac atcctcttgt aggtatgttt gcagcttttg caggtgttgc agggggattt
     9841 gcagcaaatg ttatgcttag tactacagat gtattgttag ctggatttac aataccagca
     9901 gctcaaatga tggacccatc ttaccaaggc aatccagcaa tgaatcttta ttttgctttt
     9961 atatcagcga tagttttaac ttttgcagga gcatttgtaa ctgaaaaatt tatagctcct
    10021 cgttttacaa aatataatga ttcaacaaat gataaggata tacaggaatt gagtgaactt
    10081 gaaaataagg gtataaaata cgctttatta tcattgttag ttgtagtagt agtgatagtt
    10141 gccttatgta taggtgacaa tgcatttatg aaagatccag agactggttc tatactatct
    10201 agtaatgcac ctttaatgaa aggtatagtt cctataatca ctattatatt tttaactcca
    10261 ggattagtgt atggaaaagt aagtaagaag ataaaaagtg acaaagattt agtatctatg
    10321 atgggtagta gtatgagtga tatgggtgga tatatagtcc tagcatttat agcatcccag
    10381 tttattaatt tatttaatct tagtaactta ggaacaattt tatcaataac aggagcaaaa
    10441 ttactagcag agtcgggaat acctagctat ggattgataa taggatttat attgctatct
    10501 ggatttatca atttatttgt aggaagtgct tctgcaaaat gggcgatttt agcacctata
    10561 tttgttccca tgtttatgct actagacttt aatccagcat taactcaaat agcttatcgt
    10621 attggagatg cttcaacaaa tccaataagt ccactttttc cgtattttcc agttatattg
    10681 gcctttgcca gaagatatga taaagatata ggtataggta ctgtcatatc gaatatgatt
    10741 ccatactcag tggtatttac attaatagaa ataataatat tgttactatt catgggtatt
    10801 ggtataccat taggacctgg tggaggaata tcttacgttt tatagaccgt tataataaat
    10861 ttatcaatga agattaaact aataaaatat attctttaat taagaggagg attattatgt
    10921 taaaaacaac aaaaatttca gacccagagt tatacaagat tgtagctgat gagttggtta
    10981 gacaagaaca caatattgag atgatagctt ctgaaagtac agcaccaaca gaagtacttg
    11041 aattaagtgg atgtgtattt acaaataaaa cagaagaagg ctatccaggg gctcgttttc
    11101 aagcaggttc tgaagaagca gataaacttg aaaccctagc aataaaacgt gcaaaagaag
    11161 tatttggagc agaacatgta aacgtacaac cgtattcagg ttctacagct aactattgtg
    11221 tatattcatc aatattaaaa ccaaatgaca ctgttttaag tatgcgtctt gaccaaggtg
    11281 gacacttgac acatggtagc gcagttaatt ttttacatga catttataaa tatgaatttt
    11341 atggggtaga cccaaatact ggacgtatag attatgatgc tcttgaagca aaggcaaaag
    11401 aatgtagacc aaaacttata attgcagggg caagttcata tccaagatta atagattatg
    11461 aaagaatatc aaaggttgca aaagaagtag gagcttactt tatggtagat atggcacatg
    11521 ttgcaggatt agtcgcagcg aaagtaatac ctagtcctgt accttatgca gattttgttt
    11581 cttcatctac gacaaagact ttctgtggac caagaagtgg tattgtactt tgtaaagcag
    11641 aacatgcaaa gaaactcgat aaaggtgttt ttccaggaac attagggtct atacatctta
    11701 atacagttgc ggcaaaagcc ttttcactat tatatttaag tacagataaa tttaaaaaga
    11761 taatggagca agttgtagta aatgctcaga ctctagcttc tgaactgatt tcacatggtt
    11821 ttagtattgt aagtggaggt actgataatc atatagtaat ggttgactta agaagtaaga
    11881 atcttacagg aaaacaattt gaaaaagctc ttgaatatgt aggaataaca gttaataaaa
    11941 atgttatacc tgacgaccca caatcaccat ttgtaactag tggtattcgt ataggtctta
    12001 catcaatatc tcaaagaggt ctgaaagaaa aagaagttat acagatagca gggataatga
    12061 ataaggtagc agaaaatatt gataataaag aagtattaga tgaatgtaaa gcagaggcac
    12121 aagaacttat atctaagttt ccattatatc cagaagggta ttttgaagat taaatatttt
    12181 gatttgtata gctcacaatt tataaagcct gtaacataaa atttaataat atataaccac
    12241 tataaggtgt tgattaatca acaccttatt ttttttgaaa agtaatatac aagctatcat
    12301 aagcatataa ttccttatag ataattaaca agggggataa agttatgagg tattataata
    12361 aacctaccaa agaagtatta aaatacttaa aaacaaatcc agaaattgga cttgatgata
    12421 atgaggtaga agaaaggaag cttagatatg gattaaatga atttactata aaagaaggta
    12481 gaacattttg ggacgagttg ggagaaagtt tgacagaacc tatgatatta attctcatag
    12541 gagcagcagt aataagttct tttgtgggag aattacatga cgcattaggt attttagggg
    12601 ctatttttat tggaatttct ataggcataa taactgaagg aaaatctaaa aaagcagctc
    12661 atgccctgtc aaaattaaca gaaaatatag aagtaaaggt gcttagaaat gggaagataa
    12721 ttaaaatatc aaaaaatgat ttagtaccag gagatattgt atatatagaa actggggata
    12781 tgataccagc agatggaaga cttatacagt ctataaattt gaaacttaga gaagatatgc
    12841 taacaggaga atctgatgat gtagctaaga atgctgatgc tgtattagat atggaagtag
    12901 tttattctaa aacagaaata atcgaacagg atgcaatacc tgcaaagcaa gtcaatatgg
    12961 tatttggagg gactcttgta gcatatggta ggggcataat ggttgtcact catactggtg
    13021 ataagacaga gatgggtaag atagcacaga atttatcaaa tgaagaccag caaactccac
    13081 ttcaaattaa attagggaag ttgggtgcta aaatagcagg aatatcaggc ataatagcta
    13141 ctttattgtg tatgtttatg attatacaga tgcagagaaa aggtatgtta attttagata
    13201 caagcagtat attatctttc ttacaatcat tagaacctgc taaaaatgca tatatggttt
    13261 gtattgcatt aatagttgca actgttccag aaggtttgcc aactatgata aatattacct
    13321 tagctataac aatgcaaaag atggcaaaga taaatgcatt agtaacaaag aaagaagcct
    13381 gtgaaactat aggttctgtt tctgtaatat gttccgacaa aacagggact ttaacacaaa
    13441 acaaaatgat ggtagaggta gcatatgttg atggtaaata tataagtgga ggagagtatc
    13501 aaagtaatag ctattttgaa caaaactgta tagtaaactc aactgcagat atagaaaaag
    13561 aagataattc atttaaatat ataggaagcg caacagagtg tgcattactt ttatatcata
    13621 atgataagaa ctataatgaa atgagaaaac aaacgtattt gatatcacaa ataccattta
    13681 gttcagaaga aaagaagatg tcaacattaa ttcgtcaaga ggattcagat atattacttt
    13741 caaaaggtgc tccagaagta ttattaaaaa aatgttctta tgtacaacaa ggaaaaaata
    13801 ttgttccaat aactccaaaa gtagaaaaaa gtattttaga tgagattaag aagttacaaa
    13861 ttaaatctat gagaacatta ggatttgctt ataaaaaaat gagtaattct aaaacagaag
    13921 ttgcaatgac ttcagaaggt gaattgaatt taataggaaa ttcaagaagt tatatgaagg
    13981 aagataattt agtttttagt ggatttgtag gaatagtgga cccattgaga gaaggggtca
    14041 aagattccat tgataaagca tttaatgcag gagtagatgt aaaaatgctt acaggagata
    14101 atataaatac agcaacagct attggaaatg aattaggatt attaaatgat gggaaaaagg
    14161 cagttgaagc aacatatata gatgtattga cagataaaga attgagagaa gaaatcaaag
    14221 gaatatctat tgttgcaaga agtaaaccag atacaaaaat gagaatagtt tcagcacttc
    14281 aaaaaagtgg cgaagttgta gctgtaacag gtgatggtat aaatgatgcc ccagcactta
    14341 gtcaagcaga tgttggaatt gcaatgggta tttcaggaac agaagtatct aaaaatgcag
    14401 cagatataat attgactgat gatagcttta gtactatagt agagggaata aaatggggaa
    14461 gaggtatata tgagaacttt caaagattta tacagtttca gcttactgta aatatagtag
    14521 catttataat tgctataata tctcaactta ctggtaagga tatgccattt actacaatac
    14581 aattattatg ggtaaatata ataatggatg gtcctccagc cctagcatta ggattagaac
    14641 ctgttagaga ttatgtattg aaaagaaaac caataaatag acattctgga ataatagcta
    14701 ggtctatgtt tgtaaatatt attataaatg ctattcttat aattacaatt gtatttactc
    14761 aatcagcttt taatatattg ggagctacat cagaagaaca aggtactgtt atattttctt
    14821 tatttgcatt cagtgccctt tttaatgcat taaattgtag agagtttggt ttaaatagta
    14881 caataccaaa tttctttaaa aataaactgg cattgcaaat aatagtagtt acagggataa
    14941 ttcaaataat atttacacaa gtgttccaga gtttctttaa ttctgtttct ttggactttg
    15001 atatgtggat aaagataata ctatttgcat ctactatcct attatcaaat gaatttgtaa
    15061 agctaattct tagaacaatg aaaaatagta gaactatgaa ctttaatagt aaaaattaat
    15121 tcttatgaaa ccgtaaagta ctatatattg taactttgcg gtttttgttt gtttaatgtt
    15181 gttttatatt accattttca tatttaattt tttattaatc tgaacacaat cttccattgt
    15241 aattgcttca tctgtgtcaa cacattcaaa gtcaatatct aatttttgta atattttgta
    15301 tgcctctttt tgcaaaggag tatcaaattt aataggtgat tttatgtaaa tatcactgat
    15361 aaaaaacatg atttatttca ttcctttcca tttaaaatct tatagttata gtgtatcata
    15421 aggttataaa atatagataa atatagtata aatttaaaat atgtacagac aaaacaaaaa
    15481 aatcatattc taagtaatac ttgggaaaaa tgatattatc ataataaata atctttgtaa
    15541 aaacttatat agaatctatt aaacagacac taagccataa aaactttacg aaatataaaa
    15601 ttcgtatcta aatagatata tagaatgata ttttactgga gtagagtgat ttttaagaat
    15661 tttatatata aattatgtta tcataaataa agatatagat tataaaattt gttaataaaa
    15721 taaagggcag ggaggtaagt ttcatgtttt ccaaaatcct agtcaaaaca tttatcagag
    15781 atagcgaaaa cgttcaaaat acagatgtta gaaataagta tggttatgta gctggtgtag
    15841 taggtatatt atccaattta cttttatttg tgataaaggt attcatagga atgctcacat
    15901 ctagtatagc tataatggct gatgcattta ataatctttc tgatatggca tcttcagcaa
    15961 taactatgat aggttttaaa ttagcaagta agccagctga taaagaacac ccttttggtc
    16021 atggaagaat agagtattta tcagcattaa tagtagcatt tatggtgatg ttggttggac
    16081 ttcaatttgt aaagtcatct atagaaagga tagtaaatcc aattcctgta aaatttgaag
    16141 taataccact tatactttta attgcatcta taatgattaa aatctggttg agtcgtttta
    16201 ataaatttat gggaaataaa attgattctt cagccttaaa agcagtttca cttgatgctt
    16261 taggagatgt atttacttca agttgtgtag ttatatcatt tattgtagct agatttacta
    16321 attttcccat agatggatat gttggtatcg tagtatcatt agtgatactt tatgctggtt
    16381 tctctcttgt aaaagatact ataaatcctc ttttaggaga agcaccggat gaagaaatgg
    16441 taaattctat tatagagcta ttgttatctt ataaatatat aataggtaca catgatttaa
    16501 ttattcataa ctatggagtt ggtagatgta ttgcatctat acatgcagaa attccatcca
    16561 atatagatat aatggaaata catgaaatta tagatactgc agagagagaa atatctgaaa
    16621 aattagatat atatctagta attcacatgg acccaatatg tcttgaagat aaagaagtta
    16681 tgtcagccaa aaaagaactt gaagaaatac ttaagaaaaa tagtcttgta aaatctatgc
    16741 atgattttag aatagttgga aaaggaagca agaagaattt aatatttgat atagtagtta
    16801 atccttccga attttccaaa aacatgtcag aagatgactt aaaagaagat ataacaaaat
    16861 tagttaagga gataaatcct gaatataatt gtgtaattgt tgtagataag gattttattt
    16921 aattattaat taatctcatc ataaggtaaa aaatgtaaaa aggatagaca atagtataga
    16981 ggcataatat aatagaagta tataattgta tttttaatat aaataaagca attttaattg
    17041 tttttatgaa atattataga gatgttgaag tatattctat tattgggcac cttatggata
    17101 tactgagtca gtggtgcaac cggctatgaa tataaattta tttattttca tagctttttt
    17161 tgtataattt tttagtaaaa attactttat atatgatatt aatggaagat tagattataa
    17221 attataggta acctaaaggg gtgagattta catgaagaaa ggaaatagaa aggcattatt
    17281 aattagttta attatgattt taagtatggt ggtgtctaca atatatccaa ctgtatctta
    17341 tgcttcagaa ttaggagaga atagtcagat tcaaagtggt tcaactaatt catcaactgg
    17401 agaggagaag gaaagcgaca ataaaaagcc agaacagact ccagagaaag ataaggcaac
    17461 agataataaa aaaccagaac agaccccaga ggaagaaaaa ccaacagata ataaaaaacc
    17521 agaacagacc ccagaagaag ataagtcaac agataataaa aagccagagc aaactccaga
    17581 aggagaaaag ccaatagata ataaaaagcc agagcagata caagaagaag ataagtcaac
    17641 agataataaa aagtcagaac aagctttaga agatgaaaaa ccactagaca ataaaaatac
    17701 agaaaaaact ccagaggaag ataatctatt agaagatgaa aatttattga aagttttaga
    17761 agaagaactt aatgaagaaa atgaagacta tggatttgtt gtaaaaatta ataacaatac
    17821 aatagaaacg gaatcaatga aaaaaatatc atttaattta acttatactc caacatcaaa
    17881 aggaatacaa gcaggagatt caataacttt taaggtgcca gatgtattca ataaagttaa
    17941 tttagattat acaagtgaat gctttgataa aacagaaagt aatggagagt atacacttac
    18001 atttagagaa ttacctaatg gccaaagtgt aatgcaaggt aaaataggtt tggaagcata
    18061 tgttaaaaag gttgatgaag atactaatgc aaaaatacat attgaaacta ctggaaaaat
    18121 agaaagtgga agtggagata tagatgtaga aataaaacct ggagataaaa cggatgttcc
    18181 agatgctaaa ggaatactta aaaagcttgt tgaaggaaaa aaatcaacta ctgtatttat
    18241 gccagttaaa aataaggata taaattacag tatacaagta aatgaaaagc aggaagagtt
    18301 aaaagatatt attttgtatg atgagcttcc tgaaggattg acattgataa atggttctgt
    18361 aagtgtagta acaagtgatg gaaaagaagt tagtgatttt aatattgaac agagtaaaaa
    18421 ttctatttca gttaattttg gaaatataga taaatcgtat actgttaagt ataaggctag
    18481 aatcagtgac aaaaatgcaa aacatggtaa taaatataag aatgtagcta ggattgaatc
    18541 tgatggtaag aagatacaag aagatgatgc tactgtaagt atatttgata gaggagatga
    18601 ttatcttctt actaaaggac attcaggagc aacaaatatt actcaagttg gtcaagtaat
    18661 aaactatcag atttcaataa atgatgataa atcaccaata agtaatgttg ttataacaga
    18721 taatatacca gaaggcatga gattaactac ttcaggagaa gctgggcatg attttagagt
    18781 tgtagaaatt ccaatgaatg ggagctggac tccttggagt aaagaaaaaa ttgccaataa
    18841 tatttcatat gaagtagaag aaaaaagaaa tgaaagtggg caagtagata aagttataac
    18901 tggatttaca attaatctca gtaaagaaga agtggaatca aagtttttta tagcttatac
    18961 attaaaggta attagtattg aagatagcta tataaataga gctgttcttg atgcaaataa
    19021 tagtgaaatt gataaaaatg atgagataaa ctttaagaaa aattctggat taataagcgc
    19081 aaaaaaagaa gttgataaaa aagttttaaa cagtagtgat aaccagattg ttaaatacaa
    19141 aatcaatatg tcaacttatg gagtttatga tgcaggacaa gtaaatcttt tagatgaagt
    19201 aaactcagtt ctagaaatat ctaatataaa atattctgat aatcttgaac ttaaaaaaga
    19261 agctggagat ggaaaaaata caataagatt agtaaataaa tacgaattta aacagataaa
    19321 agaaggggaa cctgtgcaaa gttgggttac atttgatgca aacttcacaa atgtaaaagt
    19381 tggggagact atacgaaatg tggctcagat taatggttca agtcctccag gagtagagac
    19441 tactaagcaa ggttatgctt ttgaagctaa aaaagtggat gctttagata aaaatgtact
    19501 ttcaggtgct aagttcaatt tagaagatgc atttggaaat atagtagtta aggatttagt
    19561 tagtgatgaa gatggtataa ttcaatctag tgtaaaaaac ccaggaacat attatttagt
    19621 ggagataatg gctccacgtg gatatgaaaa actgaaggat aaagttaagg ttgaaattgg
    19681 taatgaagat attggtaaga tagtagatat tgggaatata gaaaacataa gacaagaaaa
    19741 tccaccagta aatccaccag caccaccaaa tacagatgaa ccaatggtaa atccaccagt
    19801 accaccaagc acagataaac caaggaaacc atcaagttca tctgatacag aggatactat
    19861 agttataaat ccaccagtac caccatcaga agatataata aatccaccaa taccagaggt
    19921 attgaatccg ccagtaccac catcagaaga gatgatagaa actcctgtta aacagattat
    19981 tccaatacca gaagtagtaa agccatcagt gtcagaagaa aaaaataata gagttaaaga
    20041 tgatactttg gtaaatccac cagtaccacc aaaaacaggg gatagtacta caattattgg
    20101 agaaatactt ttggttatag gagctattgt aggacttatc gtattaagaa gaaataaaaa
    20161 tacaaattag accctaacat tataaaaata taagttatat taaaaaatat cttctaaaac
    20221 aatatttaat aatgaaaata ttgttagaag atattttttt ttgaagattt atgtaaaaaa
    20281 ataataactt tttagaaaaa agttccaaag ggatatagac aattaaaaag atataagcta
    20341 taatatagat ataaaattaa atgaggcaaa actagggaaa cctagtcacg caaagctata
    20401 ggggctaaag tttagtaaac catgccagcc agttgccgaa aagagtaaaa atttcttttt
    20461 gtatatacaa agatactctt gggtatcttt tttattttga aatttattac tcccggccta
    20521 actcaaagga gggaataata tgagaccaag taaaaaatta ttaatagcta taatatcaat
    20581 attcttaata agttcagttc cagtaagtgc acatgcagat agtactacta tacaacaaaa
    20641 taaagacaca cttagtcaaa tagtagtttt tccaactgga aattacgata aaaacgaagc
    20701 taatgctatg gtaaatagat tagctaatat agatggaaag tatttaaatg cattaaaaca
    20761 aaataactta aaaataaaat tattaagtgg aaagttaaca gatgaaaaag aatatgctta
    20821 tttaaaaggt gtagttccta agggatggga aggtactgga aaaacttggg atgatgttcc
    20881 aggtttaggt ggaagtacag tagctcttag aataggattt agtaacaaag gtaaagggca
    20941 tgatgcaata aatttagaat tacatgaaac agcacatgca atagaccata tagtattaaa
    21001 tgatatatct aaatcagcac aattcaaaca aatatttgct aaagaaggtc gttctttagg
    21061 taatgtaaac tacttaggtg tatatccaga agaatttttt gcagaatcat ttgcatatta
    21121 ctatttaaat caagatacaa atagtaagtt aaaatcagct tgtccacaaa cttatagctt
    21181 tttgcaaaat ttagctaaat agagttttaa taaatttaaa ataaatataa aagagatgct
    21241 attataatag catctctttt atattgcgtt taaatctaat aaattttgtc ttatgtataa
    21301 ctatgataga ttatcaatta ctactcctta tacaaggctt caacgtgttt attatgcatt
    21361 tgagtaatta tcgtaggtat aatagaaatt attgaaccaa ttactatcaa taaaattcct
    21421 ataaccatgt ttatagggat aatttcttta agtattaata atgctagtat tggagatatt
    21481 actggtttaa agaagaatac taaagatgta agactagtag atgtagcctc cattgccata
    21541 aagtaaagcg cataaccaac acctgtaaca aatacgaata cataaatcat aatgggcata
    21601 ttgtgtaagg tatatccact aaaaataggg atatttgcaa atacatgaag cttattttta
    21661 tttaatatat ctgcgataaa attaatttta gtgagtaaaa ccaatatcaa catttccaaa
    21721 ctaccaaata taaagctaaa gcaggttaca actattccac caaattttaa ggtttttctt
    21781 ttaccaaata cagtatacat tgaaaaaata atagcagaag ataatgttaa gaatattcca
    21841 ctaagagtta gttttgtatg caatggattt attataacta taattccaag aacttctaat
    21901 ataagtgaaa ttacattgtg cctatatatt ttttccttca aaattaagta agctaaaagc
    21961 attacaaaga ctggattaca actgaacaaa acagcaacta cagatgcctt tgtgtttaga
    22021 acagccagtt gatacaatga catacttact actacacaca taaaacctaa aaatgcaaag
    22081 tattttaagt ctgattttgt aatagaaaga ctaagagctc ttaatctttt tacagcaaaa
    22141 ggtagtaaga ctaaaccgcc aacaaaaaat cttgataaag ttatttgtaa gggattaaag
    22201 tcattagtga cagtttttag actaatttcc attgaactaa aaaataaggt agtcagtatt
    22261 atataaacat aacctttttt caagtcaaac acctccttgt aatttatatt agtactatag
    22321 ataagaatat tcaagcatat tggaataata tggtatgtat aaaatatagt acataaaatt
    22381 ataaactacc ttaatataga atgtaagtat tctatactaa ggtagttaat tatttgaatt
    22441 actttgattt agttatttta aattatataa tttggctaat ttttcaaatg ctggtaaaga
    22501 acttttaact ttatcataag atacacaata tgatactcta aaatgacctg gacaaccaaa
    22561 agaagaacct ggtactaata ataaattaaa ttgttttgca tcattgcaga attttttatc
    22621 atcttcaatt ggagcttttg ggaacaaata gaatgtacca ttaggtttaa cacaagaata
    22681 tcccatatcg attaaactat tatatagaag gtcaaaattc tttttataaa tatttacatc
    22741 aacttctgca tctaaacttc ttgcaataac cctttgaaaa agagaaggag catttacaaa
    22801 tccaagtatt ctatttgcaa catttaatga agacataaca tcatcaaaat catccatttg
    22861 cccattagca acaatatatc ctatacgttc accaggtaga gataatgatt tactataaga
    22921 gtatcctata aatgaatttc tatagtattt tagtacacaa ggaactttgg caccatcata
    22981 aattatttct ctatatggct catcagaaat taggtatatg cttgtattaa attctttttg
    23041 ttttgaatcc aataattctc caagattttt taataattct tcagaatata tgacaccagt
    23101 aggattattt ggagtgttta ttattaaagc tcttgttttt ggagtaatag cattttttag
    23161 agcatctaaa tctggctcaa aagtctctat atttgtagga acttctatta attttccatc
    23221 atagttttct gtataatttt tatactcacc aaaatacggt gcaaaagcaa taacttcatc
    23281 accaggattt aaaagagttt ttaaaataat atttagacca ccagcagcac cacaagtcat
    23341 aattaaattt tctcttgtaa gatttaatcc atctttttta tttatgtgtt cagcaatagc
    23401 atctcttaca tcctcatatc ctgagttgtt catatatcca tgaactaaat ttggagattc
    23461 ttcatttaaa atatcattta taactgcttt aatagtctct ggaggttcaa cacttggatt
    23521 tcctatacta aaatcaaaaa cattttcctc accataaata tcagaaagtt tctttccttc
    23581 ttcaaacata gctcttataa cagaactatt tgcaactaaa gtttgcattt tattagatat
    23641 catatgtaac actccttttt atagatttat attgcttatg tatatattac catattttta
    23701 ctgaatttag acaattttcg aaaaattact tgtttgaaaa tataaacttt tatataaaat
    23761 atatttatgt acagtgcata ttaaaaattc caatttctaa ttttatcatg attattttat
    23821 ataatagttt tttaaagaat taataaaaca tagatttaga gaataaaata ttcttgacta
    23881 tattaattgt ttaatataat atagttgata tagtcaaaca tattataatt atagaaggga
    23941 aatttaagat ggattttaaa aatttacagt atgaaagttt gtcatatatt atttgtaact
    24001 tgcaaaaaaa ctttaaactt tattgtgaaa aatgtttaaa accatataaa cttacgaatg
    24061 gtctatattt ctatttaatt tatattaata aaaataggaa ttgttctttg aatgatgttt
    24121 ctacagaatt tgaagttgat aaagctcata caacaagaac tatatctagg ttagaacaag
    24181 atggatacat agaaaaaatc caaaacccta atgatagtag agcttttcag ttgagagtaa
    24241 cagataaggg tgaggaagta ttaggggata taaaaaacat attttcaaag tgggataatc
    24301 acataaaaaa agagttctcg gatacagaat ataaagaact ggtaaagaat ttacatgtgg
    24361 taaaggatat aaaaacagct gtggaggaag aataggatgt attgtaattt attaaaacca
    24421 attaaaattg gaaatttgga attaaaaaat agagtatctt ttgcacctac aagcatgggt
    24481 cttaaattgg aagaaaaaat taaaaagttt tctgatattg ctaagtcagg agtagctctg
    24541 attactctag gagatgtatc tattagacct agttttcata aggtagctat aagcctatcg
    24601 gatgaagatg gagtcatgaa atacaagaaa attgtagatg aaatacacaa ttctggtgca
    24661 aaggtatcag cacaactatt ttgttcagat tatgatgtta atttaattaa agatactatg
    24721 aagatgggaa ttacttcaca tgatgagata aaaaaaataa tgaatgacgg agtaaaagat
    24781 tacataacaa atatgcccaa agaagaaatt aaaaatatta taaatttatt taaagtaaca
    24841 gcattgaatg caaaaaaagc aggttttgac atgattcaaa ttcatggtga taggcttgta
    24901 ggaagttttt catcaagtat atttaataat agaaatgatg aatatggtgg aacgtgtgat
    24961 aatagaagta gatttgcaag cgaaataatt agtagtatcc gagatgaagt taaggacatt
    25021 ccaatagact ataaatttgc aataagacaa gaaaatcctc attatggaaa tgcaggagtt
    25081 ttattttcgg aagttgagta ttttgttaaa aaatttgaat ccctaggtgt aaacagtttt
    25141 catgtaacac ttgcaaatca ttcaaaatta gaagatacta taccaaccaa taatcatcct
    25201 tactttaaag atgaaggctg ttttttatat ttagctgatg aggtaaagaa acacacaaat
    25261 cttccagtat gtggggttgg aaaattgtct agcccagatt ttatagaaag tattatttca
    25321 aataatagag tagatatggt ttctatgtca agacaattac tagcagatag taattggtta
    25381 cagaaagtaa aagatggaag agtagatgag attaaaaagt gctgttattg taataagaag
    25441 tgtgcagatg ctttacaaac acgctctcag tttggatgta tactagatta gaatatcctt
    25501 ataaaaataa ataatttgtt aaggttaata tatttttggt gatttagata agtatttaga
    25561 taaagaggag gagacatcat gaaaagttta gttttatatt caagtttaac tggaaatact
    25621 aaaaagattg cttatactat atatgatgaa attcaagaag aaaaagacat taaagatgta
    25681 aatgaattag ttgattatag tattgactat gaaaattatg atatagtatt cttaggctat
    25741 tgggtggaca agggaatttg tgataaaaat tcaaaacaag tgcttgaaaa tattcacaat
    25801 aaaaagatag cactatttgg aacaatggga gcaagtaaaa aaggaagtta tggagctagt
    25861 ataatagaaa agatagaatc aattatccca aaagataatg aaatattagg ttcattcata
    25921 tgtcaaggta agatagcaga aggattgaaa gcaaaatata aagagatgtt aaaactttct
    25981 cctgacaacg aacatattcg ccaacaatta aataaccatg aggaatctca aagtcatcca
    26041 gatgaacaag aaatatatga agccagtatg tttgcaaaaa atatgatgat taaagctagt
    26101 attgtgtagt agttaaacaa taagttcata taagatatag aataaataat ttagtaaaaa
    26161 agtatagcaa ataatttgct atgctttttt tatgaaatca aaaaaataat aaaaatactt
    26221 gactgtaaac cttgtttata cattatttta tagaaaataa gatagtttta aatataaaaa
    26281 aagatatatt attgaaagga gatattattg tatgaaaact tataaaagat aattagtttg
    26341 attttaagat atactaaata tacaatttta tagagtgaaa atatttaaga ctatattata
    26401 gttataaatt aatagaatag agaggtagtg tttaaaatga caattaaaga agttgaagaa
    26461 aaaacaggat tgacacgctc taatattcgt ttttatgaga acgagaattt aattaaaccc
    26521 cttcgtaatg agcgtaatgg ctatagagaa tattcagatg aaaatgtaga gaacatcaaa
    26581 aaaattgcat atttacgtac attaggaatt actattgatg acatccgtaa tattatatta
    26641 caaaaagtat ctttagttga agttgtagag aaacaggaga aagttctcga aactaaaata
    26701 tcagacttag aaaatgcaaa aactatgtgt aataaaatgc tagaatctga tgagctaagt
    26761 tatttgaatt tgaaagtaga gcaatacata actgagttgc cagaatactg gaataaaaac
    26821 aaacctgttt ttaaacttga ctctgtaagt ttcttatata tatggggagg gaaaattacc
    26881 tggggaatta ttactattgc ttgtttgttg attgcaataa cagtatttcc aaagttgcca
    26941 cctcaaattc ctatccaatg gaataatggt gtagctaatt atgtagataa aaaatttatt
    27001 tatgcctacc ctgtcatttg tgttgtattc agaatacttt tacgtccatt tatatggaga
    27061 tggttacaaa tacatgcttt ttatagtgat actattgcag attatttaac gaattttatg
    27121 tgttttgtag cgttgtctat agaaatattc tctatacttt ttatttacaa tattgccaga
    27181 catgttacaa taattatttt tatagatata cttgtattta ttgctttact gataataggt
    27241 gtgtataaat tatttaaaaa atacaattaa tttttaaaaa taagtaataa gtgatagtgt
    27301 aaaaagtagc cactgcaaat tatggattgt agtggctatt ttattatttg tatatgaaat
    27361 tattttgttt ttc
//
LOCUS       DAGSWI010000048        27097 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.048, whole genome shotgun sequence.
ACCESSION   DAGSWI010000048 DAGSWI010000000
VERSION     DAGSWI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.048"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            33..836
                     /locus_tag="MJF80_001838"
     CDS             33..836
                     /locus_tag="MJF80_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HBZ0280501.1"
                     /translation="MNKVLNWPGAKWSMSKNIVGILPKHNIYLEPYFGSGAVFFNKKA
                     CNTEILNDADKQIVNLFKCIRDNPNELMNAIYFTPYARDEYMNCNILETDNDIEKARK
                     FIIRTNMARGGIQNYKTGWKHTGPKESKKCFQKVVGKWNKLPKIILDTAIRLKDAEIE
                     NRDAVELIKKYNKTDCLIYADPPYLLKTRSQKMYNVEMETEEEHEKLLKTLLKFSGYV
                     ILSGYDSDLYNEMLNGWNKMEFKACAEQGKRKEMLWTNFETTKQLSLFK"
     gene            849..1256
                     /locus_tag="MJF80_001839"
     CDS             849..1256
                     /locus_tag="MJF80_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBZ0280502.1"
                     /translation="MRINFTIDGEPVGKERPRFNLATKRTYTPNKTKSYEELIKWLYQ
                     SKVKHYFEGYIKMTLRCYYSIAKSNSKKVKEQKRNNVLRPSKKPDIDNVVKIIADSLN
                     EIAYKDDTQIVEVVASKYYSDKPRVEVILEDVI"
     gene            1358..2206
                     /locus_tag="MJF80_001840"
     CDS             1358..2206
                     /locus_tag="MJF80_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBZ0280503.1"
                     /translation="MNENINKEITVLGTLEIEGMKFHNIEGGFGEHKKAMLVKDIAEI
                     HNRESRQINELINKNRKRFKDGKDILDLLGVGLDDTKIKELGFTQQSINSYRGLKNKG
                     LLSGIYILSERGYAKLLKILEDDIAWELYEKLVDGYFSMRKELNNPLLSASKELQTIF
                     MLDKKQEVLETKIENVNEKLENFMDDAPLFNIECESIVKEVKKVATKSLGGHGSKAYK
                     NKSLRGKVYNDIYHQIKREFGVDSYKAIKRCQLNKVLEIVNNYKLPIVFEEEIRLLNS
                     QLSIVS"
     gene            2309..2797
                     /locus_tag="MJF80_001841"
     CDS             2309..2797
                     /locus_tag="MJF80_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280504.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSTVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKVMDMLYPRIKENELPLF
                     KN"
     gene            3248..3445
                     /locus_tag="MJF80_001842"
     CDS             3248..3445
                     /locus_tag="MJF80_001842"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280505.1"
                     /translation="MSNLREKIIKELNEFNIKADNDFLEYAVEHIKKFMNEGIADEEL
                     LIRGVVLGASYVVNIENKDLD"
     gene            3492..3749
                     /locus_tag="MJF80_001843"
     CDS             3492..3749
                     /locus_tag="MJF80_001843"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280506.1"
                     /translation="MNDYKEKTEPKLNILVQSKVDILCSNSSTMNGVDTSIDIKLSLD
                     NRVIGNLTNEDIKKEISTSFNKCLKEIDKKICSKEVINKLI"
     gene            3920..4621
                     /locus_tag="MJF80_001844"
     CDS             3920..4621
                     /locus_tag="MJF80_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0280507.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWANE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELIQEISIKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLKKSDTGDDSKIWV
                     EAIQNIAMKRGVNNG"
     gene            4614..6023
                     /locus_tag="MJF80_001845"
     CDS             4614..6023
                     /locus_tag="MJF80_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase B"
                     /protein_id="HBZ0280508.1"
                     /translation="MDKALLTLLDCYWDNPVWFAEDMLNFKADKWQSDVLMALAQTPK
                     VSIRSGQGVGKTGLESIATVWYLSTRPFPKVVATAPTRQQLYDVLWAEIAKWLSNSKV
                     EKLLEWTKTKVYMKGFEERWWATARTAVKPENMQGFHEDYMLFVVDEASGVADPIMEA
                     ILGTLSGAENKLLLCGNPTRTSGTFYDSHNRDRDLYKTFKVSSLDSPRTSKDNIEMLK
                     RKYHEGSDPWRVRVLGEFPKGESDSLISLEAVETSTIREVNISNDYILNIGADIARYG
                     DDETIIAPRIGGKVFDLLTYSKKDTMETVGNILRAVDKFKNMYHQINRVKIKTDDDGL
                     GAGVTDRLKEVIRHERLKYEVIPIQNGSSAIEKDKYYNKASEMWDNMREELDANLSSF
                     IQNKEAIIQLPNDDKLIKQLSNRKYTVDSKGKIQIESKKEMKKRIGESPDRADAVIYS
                     FAENNNTDLSLLKGGSVWG"
     gene            6014..7516
                     /locus_tag="MJF80_001846"
     CDS             6014..7516
                     /locus_tag="MJF80_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBZ0280509.1"
                     /translation="MGIISYVKKLFKRPAGEIMRMSSGNIGVYKLDDSRVDYELAREL
                     YQNKNAKYKLGSSFVRPIVNSTTGFMGVPHFQIEDEEAQYILDEFVLDNTSKMLKTHT
                     DSLKQGDCYIWITREERENPLYPDKKVRLIYNFISPEEVKEIILDPTTKEPIAYILES
                     QNEWTDLGENKRKAKVKQIITAESRFVEVEGDKIEGLEEGETPNVWGFIPIIHFKNEA
                     DETLKYGQSDIEPIEPLLKAYHDVMLHALKGSKMHSTPKLKLKLTDVASFLAHNFGVE
                     DPVKFAKEGGKINLDGHEILFLNKDEEAEFVEVKSAIGDAKELLKLLFYCIVDVSETP
                     EFIFGVHTPSALASVKEQMPIMVNKIRRKREQFTNSWQLLARMVLIMSSNSSGMKYSS
                     YDVTIGWDEVNPRDDKELAETLEKVCSALDKALEGGFISEESTVNFLAQYIDTMSNYI
                     SDDPEIVGEREKIIKTKMLKYRLDDSQGLNDESNEIEKEINKIKDNNGNG"
     gene            7503..8516
                     /locus_tag="MJF80_001847"
     CDS             7503..8516
                     /locus_tag="MJF80_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280510.1"
                     /translation="MAMDKSTSELITVAGEYKKWALEARKKFIDLRLKQDDEIRTMYI
                     NITRNITKEIRKGNLSDFNKVRLKQILKQLTQEIKILNEQLVFNFDEYLNKNVETATS
                     YSKNILINAVETAQITKVTKTMIQKAFYDINIRTVEAYYTRVKDGLFLSDRIWSKCKK
                     YREDMKVILQTAVTEGQDCVKTAKMLDKYVLKGKKTLVDEYPNMIKRIGNRVPQNISY
                     EALRLARTEMTSAYGDGVLASAMINPATIGIQFMLSMAHPHTDICDEICGEDNFGLGK
                     GVYPINEAPVYPFHPHCLCIMLTVVQPLDILVGRLKNWIKNPMNDVPLEMWYQEVYGN
                     LNF"
     gene            8536..9870
                     /locus_tag="MJF80_001848"
     CDS             8536..9870
                     /locus_tag="MJF80_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280511.1"
                     /translation="MNVITGEMDSMNALISSIKPSDIPLAKDIDIEALKSIDDDPLEV
                     VVEIPATKSKRGWNYTAKSLKDIVDYTNENTLNGFLGHQKAENISTEFALPVTHWIGA
                     EMKGDKAYFRGLIDADATNLKRWIRTKRIKEVSIFGYPKLKKSAKGEMNVIGYEPLSI
                     DWTPLHRPGMPTSIVGMEMSPNGEQLDGTFEALRIDLREALKAKFSINDNNSYLYIQN
                     IRYDNNTVIYELEQNGLCKLYSIPFTIVENKINLGEEIEVIKKISYEAKGEMKGEENK
                     LEGKELIKNVKGLLQTGEISYSEVIQGIGLTKEIVTGEMEDVKSSLKAEKELREVKKV
                     LGIVGEMDTVEVAKKASKALENEKKEAWNCIVNKVIKDKVSGEIAQTLVKKMLNVEEG
                     SSEEVITGEIENILNDEFVKNTMSNMYKDNPTTTGLLNSSNNGSLTTKKNRI"
     gene            9886..10269
                     /locus_tag="MJF80_001849"
     CDS             9886..10269
                     /locus_tag="MJF80_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HBZ0280512.1"
                     /translation="MAFKGQPTPSTITQITRAKISDGKSVRVILSEGESTKTQQFYLI
                     NGFFGVAMQDGEKGDEVTLQIEQAEYETDNIVTSEAFEAGKLIYWDNTAKKFTTTSAS
                     NRLVGRVTDGKDSNNVIWFILLPQQ"
     gene            10288..11325
                     /locus_tag="MJF80_001850"
     CDS             10288..11325
                     /locus_tag="MJF80_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBZ0280513.1"
                     /translation="MAFKVISQENLLEQKRKETLQEDIPFIVNGEMEYVTKKISNGEM
                     ETLELNKPLGEMMTFSSTSNLKELLRKVVLDVELGREQVQLLYKPIYDSIADSNLPQV
                     MDAKWALQGNCVFLEHIEGEEIKFGTINAENGPVARIQTYATGFEYTKEMKDFNQTFS
                     VEILNKSIGESYNALLNHIHLSPIINFNYKASNKTAFKGETNDPIWLGIWRTLTQAQK
                     DTVIAKRQGNILMASSADQIEIEMALNGGHLLNGSMYPSIKNISTVIYYDGWEVTVGK
                     KTYSYKGVTPGKGYLIRPKRGFKELIKRDLTTEVGNADLSKLVENQIVGHCYRGAFAA
                     VEENVQEISFR"
     gene            11365..11703
                     /locus_tag="MJF80_001851"
     CDS             11365..11703
                     /locus_tag="MJF80_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280514.1"
                     /translation="MTPTSNLIEKLRLLLNDKDKKSFTDEELNLFLEEADCIYCAASQ
                     GWVLKSLQYENTVGEMYEYKVGQETYKSSSIKDLVSVAYQNAEKFKDMCTNKKEKGSF
                     MLGISTEFEI"
     gene            11700..12077
                     /locus_tag="MJF80_001852"
     CDS             11700..12077
                     /locus_tag="MJF80_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280515.1"
                     /translation="MINIDRRRKDIIRTINMNPTNITITNIKKTEIDGAFEETETEIK
                     CVVRIFNEKTAEKQISSEKQGTFSSIRTYGMLVSDDVVLDVNSRDSLEFECIYGRMKI
                     VNVYPQIVKGELCGYQCSLERID"
     gene            12079..12489
                     /locus_tag="MJF80_001853"
     CDS             12079..12489
                     /locus_tag="MJF80_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280516.1"
                     /translation="MSAFTNAINDINRKKAGMFVLCMSASAMLEGEAKANASWTDRTS
                     HARQSLNAKTLGGGNNFIIRLSHGAEYGGILEEGSKPHVITPKSAQALYWRGASHPVK
                     SVQHPGTKAMPIIKPTIDKNIGKIGNMIFRYWSD"
     gene            12490..13311
                     /locus_tag="MJF80_001854"
     CDS             12490..13311
                     /locus_tag="MJF80_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280517.1"
                     /translation="MRAGIRKALIDNIKELKGCYEPNVPNKDTKKPYMVVVQGQDNDN
                     GETIGFERSIEVWIYEGRTTFKKLDKLTKQVIEILDMNTIVDESENEAFTCIYKGTSE
                     NDIVVEEWDAIARGIRFSVIALEDKEDATSDRWVEALSKYTKDLLEIESYKDNWKKNF
                     IAPCALWRTTNVENKRINYHLIEITKTMKCHVVSKNKDEIVKFLEALETSLIIDKRVR
                     LREDKNMYLTLVSVVEDRESDMFTTGQLTVVFKMIGKIKREGPIMDKIYSNGNLK"
     gene            13324..13527
                     /locus_tag="MJF80_001855"
     CDS             13324..13527
                     /locus_tag="MJF80_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280518.1"
                     /translation="MAETINKKTNVSKQEEKYLKNDFLKNSEALGYEKMVVAGALFNC
                     KKEELTKSEFEKLIKDFLEREVK"
     gene            13529..14950
                     /locus_tag="MJF80_001856"
     CDS             13529..14950
                     /locus_tag="MJF80_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBZ0280519.1"
                     /translation="MATGTWNEKEKKEIPGFYNRFKTQAEKSANTGLKGRLAIPVKAN
                     WGEVGKVVTIKNDLRQLKTLFGDDMNYSAYKLGKLALLGNVKELLLYRLVDGKQKKGT
                     LTLKDTTENSAKDVIKLETKYPTARNFNVTIKSNLVDSDKKDFIFFENTKQLFSSSIK
                     GTIDEIVLEINSNLDNEYVIATKVADSDTILANVVNQALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVADEALQETTKAWVAKNKELGKDILLFLGGKTEDNIKQINDKS
                     KSFNDENIVNVGSSAYYENIKYTPSEVAVYIAALSVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLVLDFDDGDVIIVDDVNTFKKYVDDKNEAMGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDATGQTTVICALKKYFEELMSQGIISEFNVDIDTELQATAKADE
                     FYWKWDAVKVDVMKKIYGTGYLG"
     gene            14966..15379
                     /locus_tag="MJF80_001857"
     CDS             14966..15379
                     /locus_tag="MJF80_001857"
                     /inference="COORDINATES: protein motif:HMM:NF020949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280520.1"
                     /translation="MYNDDYIEEASFLNGSDVVILIDGVEELYMEEIKADFEQDEQSI
                     KLLGCQNEISRVGTTKGSFSLNGYKTDSKFAKLGFRSFEIIYNLSNSETLGYESIRLK
                     NCRLKKLPLINSKAGEIVKIEVEGSFRGYDLLNEL"
     gene            15463..15954
                     /locus_tag="MJF80_001858"
     CDS             15463..15954
                     /locus_tag="MJF80_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280521.1"
                     /translation="MSEIYKRELENEVVEDNDLDEEIEETNEDRLKMKEDEIIAKLLE
                     DSPVPQRTVFLDRLGIPITLKALTEKEISKIRKECTKIVKVQGRREEKLNDDEFTLAL
                     IEKGTVKPNFSNQKLLNAMKVTNAREFIKRKFLAGELSKISDQILELSGFYDEISDDD
                     IKN"
     gene            16131..20405
                     /locus_tag="MJF80_001859"
     CDS             16131..20405
                     /locus_tag="MJF80_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBZ0280522.1"
                     /translation="MREIAKKEMYHIDVVISAKGDGETKSKLSAMEKYMKQTEKRMQT
                     LNRIKVNPAIKATDKASSVVNRVNNNMNKAKKTVTARIKATDNASPVANRASNNVNKA
                     KKTVTARLKATDNASSTVNKVNNKIKEVAKPVPPVIIRGQDESSSIIDKVKAKIQNLK
                     ADTIIKIKSQADEAINTISRTKNKLQEFVSKRYEAAVKIRDEASSALRGLTGKIDSFV
                     SGAISKFARLATTAGALIGGIGVGSAVKGFATFEQSMKNTQAVSGATGKEMEALTAKA
                     RQLGRETSFTAKDAGDAMYYMGMAGWKSEQMIKAIPDVLNLAAAGGTDLALTSDIVTD
                     GLTALGMTANDTTEFVDVMAATITNSNTSVELMGETFKYVGSMGGALGVSMKDLSLAT
                     GLMASASVKGSMAGTSLRGGLVRLIKPPEEAASAIKKYGIELKKNKNGSLDLAGTIGS
                     LREKLGGLKDVEKGVAISSIFGRTAMAGWAAVVNASESDFNKLTTAIAESEGEAKRIA
                     DMKLDTLSGQFEILKSAIDDVRISVGQRLGPMTRGFVEDLIKKMPQIGDAIVGVVEKF
                     VNNFDKIKAGFQVLLPAIGSVIASVMVLKATFAFGGAIKSLSLLASTFGTAKLVAFGL
                     TVGIGAIVIAFAGMTVAISNNKTAMMDLQTRFGSFGEYVTTIMETVGGVIKLTLGNLL
                     IMLGGIGKGIGILLSDKSWDEKASSLKNLFGKTTAEIKTNTKEALSDINGETSNATAL
                     LKKSTSKELQGVTKAFSTAFDQSKNVTEKKSSDIARALTNGLKGLDDQSLTMMRGLND
                     NMAIILSGVTADMKPSDKVNKITKNLDDAFKAGKLSAQEYRSSIQETLNFISKYSADS
                     SNNLKQGMSDAFNAFKEGTNIGGLKDGATGMLNSLKATGPQALETLKGLGGKASEIFK
                     GVDFNSSIDAQKTKVLQNLNSLGLEGTQAIDTLRTIFSQASSALDTTNLKQGLSNTFN
                     SFKEGFNSGGIKNAINSMLGTINQAGPQMQQALGKMDGKMGQVFANVDFSTPIETQAS
                     KVLENLNNLGIEGPKALETVRSIFAQASSQIQGSASQTAQQANQEVANALTQGNPTVQ
                     QAGQQLGTDLTNGVVNGVQAGVPVVQQKSNELATATQNGVTNAVNSATPQIDNSNLTS
                     GIDTAFNQATATVQQGASSMYNGAKQSFTQLAQIGREAGSSLYNGATTSFNMLATNVR
                     VACSSMYNGARTSFTSLSSSAISAISAMCSSVVSQVSAMSSQIISYWNSVRATVSAPI
                     TASFNVKTTQTTVKRTVNESSGGILGNILDRFADGGVATRTSICGENGAEMVIPLTNT
                     RRSRAIELYEQTGKMLGLGSTPSQKVGNSNIVNNVRQFPLANVLDTDNKEYKEATPNS
                     VNSSNNTINLGGISINVQNSDNKEEMIQELLSQVESGIREALQDIG"
     gene            20487..21230
                     /locus_tag="MJF80_001860"
     CDS             20487..21230
                     /locus_tag="MJF80_001860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280523.1"
                     /translation="MWQKFKSMNGILKIIIGIIAIILLPITLVLLSIEFLVKAIGSKK
                     KAKIALGCILVFLTTLCTRNVYNVLSIEKEEHIPVKQVSYNDKDKGKSENNIQEQQQK
                     KEESKKLEEQKKNEEKEQNNEPTKFFTDVYLKNKNCVNMTSYEGIKSNLDIFGYKYEV
                     IEPNETTLRQINVYDNQSNDTIQFQFYENDTGEEVVTVMQYNKKDEPDKSVAISDGFH
                     TQPPKFQNHINNTNKEVNSIEEQINFMFN"
     gene            21299..21970
                     /locus_tag="MJF80_001861"
     CDS             21299..21970
                     /locus_tag="MJF80_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBZ0280524.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVNPQDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPNLYDASYCRYSELKNPIEVVATLEKWVDQAEPLRLIIT
                     GFGYNGLVTISSFSNTQTAGREEDRDIEITFRTYRELKIETLKKDTKSNTKTDLKDNR
                     PNTQTKSKIYTVKASDTLYKIAKNLLGKGSRWPEIYNIPENKKVIGKNPNIIKKGQKL
                     VIPSK"
     gene            21967..22929
                     /locus_tag="MJF80_001862"
     CDS             21967..22929
                     /locus_tag="MJF80_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HBZ0280525.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDVSLVEPKQLKDI
                     NIKKGDKIILIDIAYESKKEETIFDGVVWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     QFKENTATQRIEYYCKQWNIPYYNLANTGKKLAKVIHKTNILDMIKKDLKETATKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDADKFGTLQKIKQDEKIKNAKEAKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKSKEYYVIDVTHTLDSRPKMKLNIGSLEYIRRKFYNND"
     gene            22922..23287
                     /locus_tag="MJF80_001863"
     CDS             22922..23287
                     /locus_tag="MJF80_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280526.1"
                     /translation="MTDARFNGIARILKENMNKSVANGTFGMGCELAEITANGLKVNG
                     YKDEIQDYLVLENLTLKEDYFTFSDEALSGEYRHKHKIETPKELKPLRIGDNVLVAVM
                     GAEFVVIGRVVNAKPISSK"
     gene            23262..23720
                     /locus_tag="MJF80_001864"
     CDS             23262..23720
                     /locus_tag="MJF80_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBZ0280527.1"
                     /translation="MPNLFPQSETFETVELKNNDENELDLKGSFLFDFEKGEFVKNAD
                     GTLKKCDKVQAYKQWCQKAILTPRYKKAAYTNIYGSEIKDLIASNLSQSAKELEITRL
                     IKETILVHPYTKEVGEFSFNWLENSRLVEYEFDVLTIDDENIVIDGNIKR"
     gene            23729..24862
                     /locus_tag="MJF80_001865"
     CDS             23729..24862
                     /locus_tag="MJF80_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBZ0280528.1"
                     /translation="MERELPIPVFLTEDEDSVHERMLSNFQDVSTLEGDFIYDATRPT
                     AEQIAELKQLGLQNNLKIAFPQTSYGTYLEWLGECKGVFKNQPTKATGVITFTGVQGT
                     IITKGTIVTTIATDEKQSIEFELLETKTIGENETVDIKAESRIVGTIGNVSKGSISVL
                     LGSINGVKSVTNKDFKGGTDIEDEEHFRERVLVAEQEDKLSGASSDYIRWAKEVDGVG
                     YAYVVPEWNGAGTVKVLILDKNRKAATQELIDKVQEYIYPLNISEGENRDGKAPIGAL
                     VTVVTPDTLLINVKASFIFSNSFNEETVLNNLKTKIDKYLDKIDLGGTVSYNAIQAIV
                     GSMMLTDEGIQDFSNLTINDAKENIKLQDQVVGIGEIVNEVVG"
     gene            24859..25629
                     /locus_tag="MJF80_001866"
     CDS             24859..25629
                     /locus_tag="MJF80_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBZ0280529.1"
                     /translation="MITSKKGKEMLLTLSPIYEQSLVMNSIYEAIGSEFDNLELLNKE
                     IELQLFPQTATWGLEFWENRVGLSTNIDEDIEARRRKVIAKLQMKYIVNPNRLATIIK
                     SYTGTDVYIKENIAPYTFKVTANVDDVINYEDFKYITNKTKPSHLHWMPSFALKFTDI
                     EKFEVKMINRIFIDFRGNISNFLDGMWLLNGSKNLSAYILYNEPISLNMKNKLFVKES
                     EVFTNLKVIIKKNLYYLNGLEMLNGNKLLNAELREEVL"
     gene            25632..26012
                     /locus_tag="MJF80_001867"
     CDS             25632..26012
                     /locus_tag="MJF80_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBZ0280530.1"
                     /translation="MANAVTTDIARQKMCKARAGDITLPTIVSMAFGDGGIGNDGTII
                     APLSSDIALKNEVFRKDIENYVYPIPTTCRYSTTLLKNEAEGKNINEIGLIDSDGDLI
                     AIKSFGNKYKDSDMEMVFQIDDEF"
     gene            26117..26641
                     /locus_tag="MJF80_001868"
     CDS             26117..26641
                     /locus_tag="MJF80_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280531.1"
                     /translation="MLDNDKYLNNKIDTTKTELNTRIDTENEKQNIKIDQLIAGGSNV
                     ASTQTITIDDWVEDAESGFKSTVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIE
                     IRSETRSELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDRFVNIEEKLSGSL
                     SNIATSVNELITYC"
     gene            26656..>27097
                     /locus_tag="MJF80_001869"
     CDS             26656..>27097
                     /locus_tag="MJF80_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280532.1"
                     /translation="MQTEWTFGYNGSPQSVILKPGKYKFECWGSSGGINNSSWYTDAK
                     GGYSKGEITLKKQTTLYIYVGESGYASSSTANNTKSGFNGGGKGYLNQQVMGTYYSMY
                     GGGATDIRLVGGAWDNEQGLLSRIIVAGGGGGSYSPYTGGAGGGL"
BASE COUNT        11135 a         2986 c         5296 g         7680 t
ORIGIN      
        1 gaatgtagaa agaaggctaa gaaggtgaaa aaatgaataa ggttttgaat tggccaggtg
       61 ctaaatggag catgtcaaaa aacatagtag gtatacttcc aaaacataat atatatttag
      121 aaccatattt tgggagtgga gcagtatttt ttaataagaa agcatgtaac acagagatat
      181 tgaatgatgc agataaacaa attgtaaatt tatttaaatg tataagagat aatccaaatg
      241 aattaatgaa tgctatatat tttactccgt atgctagaga tgaatatatg aattgtaata
      301 tccttgaaac tgataatgat atagaaaaag ctagaaaatt tataataaga acgaatatgg
      361 caagaggagg aatacaaaat tataaaacag gttggaaaca cacaggtcct aaagaaagta
      421 aaaaatgctt tcaaaaagtt gtagggaaat ggaataagtt acctaaaatt attttagata
      481 cagctataag gttaaaagat gcagaaatag aaaatagaga tgcagtagag ttaataaaaa
      541 aatacaacaa aactgattgt ttaatatatg cagaccctcc ttatttacta aaaacaagaa
      601 gtcagaaaat gtacaatgta gaaatggaaa cagaagaaga acatgaaaaa ttgctaaaaa
      661 cacttttaaa gttcagtgga tatgtaattt taagtggata tgattcagat ttgtataatg
      721 agatgcttaa tggatggaat aagatggaat ttaaggcatg cgcagaacaa ggcaaaagaa
      781 aagaaatgtt gtggaccaac tttgaaacta ctaaacaact tagtttgttt aaataatagg
      841 agagagtaat gagaattaat tttacaatag atggagaacc agttggcaaa gaaagaccta
      901 gatttaattt ggctactaaa aggacctata cacctaataa gactaagagt tatgaggagt
      961 taataaaatg gctgtatcaa tctaaagtta aacattactt tgaaggctat ataaaaatga
     1021 ctttaagatg ttattattct atagctaaaa gtaacagtaa aaaagttaaa gagcagaaaa
     1081 gaaataatgt attaagacct agtaagaaac ccgatattga taatgtagtc aagattatag
     1141 ctgattcact taatgagata gcttataaag atgatacaca gattgttgag gttgtagcta
     1201 gtaaatacta tagtgataag ccaagggttg aggttatatt agaagatgtt atctaaccaa
     1261 cggaaaaatc cgttcgttaa attatgccct tagtttttca taaaaatgtg aaaaagctaa
     1321 ataaagaata tatcaaacga caaaggagag ataaattatg aatgaaaata taaataaaga
     1381 aataacagta cttggaactt tagaaatcga gggaatgaaa tttcataaca ttgagggtgg
     1441 atttggagaa cataagaaag caatgctagt aaaagatata gccgagatac ataatagaga
     1501 atctagacag attaatgagc ttataaataa gaatagaaaa agatttaaag atggaaaaga
     1561 tatattagat ttgttaggtg tcggtttgga cgataccaaa ataaaagaat taggatttac
     1621 tcaacaatca attaattctt atagagggtt aaaaaacaaa gggttattat ctgggattta
     1681 tatattatct gaaagaggtt atgcaaaatt attaaaaata ttagaagatg atatagcttg
     1741 ggaattatat gagaagttag ttgatggata tttctctatg agaaaagaac taaataatcc
     1801 tcttttaagt gcatcaaagg agttacagac tatatttatg ctagataaga aacaagaagt
     1861 cttagaaact aaaatagaga atgttaatga gaaattagag aactttatgg atgatgcacc
     1921 actattcaat attgaatgtg aaagtattgt aaaagaagtt aagaaggtag caacaaaatc
     1981 actaggaggt catggaagta aggcttataa aaataaatct ttaagaggta aagtatacaa
     2041 tgatatatac catcagatta aacgagagtt tggagtagat agttataagg ctataaagcg
     2101 ttgtcaatta aataaagtat tagagattgt aaacaattat aagttaccta tagtgtttga
     2161 agaagaaata agacttttaa atagtcaatt atcaatagta agttaaattt attcaaaaaa
     2221 aaggaaaagg agtgctttca cactccactt gtcaaaaata taaaactttt atccaagatt
     2281 attataacat aaacaggagt gtggaagtat ggataataat atcaataaaa aagaactatt
     2341 taaaaaagta gaaggtagat tacatcatta taaattttta agtgcagaaa ttaagaatct
     2401 tgaattagat atagaaagta gagaaaatga gatatttggg tgtaaggctg ttggatatga
     2461 tgaaaaagta agtccaacat atgcttttaa ttcaactgtt gagaatgaga ttataaaaaa
     2521 agaaagagat attactagat tgaaaaaact gaaaaaagat aaagaaatag aaaagaagaa
     2581 aatagaaaat gcacttacat gcttagatat aagagaagaa cattttttta aactgtttta
     2641 taatagtaga atgaaaaata gtatggttta tatatcctta gagatgaact cagataggaa
     2701 aacatgtaga tgtgtaaggg aaagattagt gtataaagtt atggatatgc tttatccaag
     2761 aattaaggaa aatgaactcc cattatttaa aaattagaaa attccccagt ttttccccag
     2821 aaattcccac tttattccct actttctccc ctttttgatt aaaaaagcat gagataatag
     2881 tattgtggaa ataaagattt ccctctcaaa acttaatatt tgactagggt ataagggatt
     2941 gccctagtca ctacgaacag actaggcagg gcgtgaggac gctgttagtt caattctaac
     3001 tatgttcaaa tattaatcaa cgtatacact aaaagtagag aaattgaggg caaaatttta
     3061 tattttgtat cttaattcag aagtctaaaa atcgggtggg gcttggtaac ctcactcacc
     3121 atgcaggtac tggtgtttaa tctaagttcg attcttagaa cttgcgacat aatatatgta
     3181 tctccctatt taaaaaggct aagtgagggt aagcttagtc ttttattttt ttacaggagt
     3241 atgataaatg agtaatttaa gagaaaagat aataaaagaa ctaaatgaat ttaatataaa
     3301 agcagataat gattttttag aatatgcagt tgaacacatt aaaaagttta tgaatgaagg
     3361 tatagctgat gaagagttat taataagagg agttgtgctt ggagcaagtt atgtagttaa
     3421 tatagaaaat aaagacttag attaatttct aggtcttttt tttatacaat aaattaaaag
     3481 gagaataaat tatgaatgat tataaagaaa aaactgaacc aaaattaaat attttagtcc
     3541 agtctaaagt tgatatctta tgttctaata gctctactat gaatggagta gacacttcaa
     3601 ttgatataaa actttcttta gataatagag ttattggcaa tttaactaat gaagacataa
     3661 aaaaagaaat atcaacatct tttaataagt gtttaaaaga aattgataaa aaaatttgta
     3721 gcaaagaagt aattaataag ctgatttgat ttcctcaatt gaagaaatta gttgttcttg
     3781 aactttttct ataattaatt ctttatctaa acttccagaa taaaaaggtt gcaataaatc
     3841 aatgtaattt aattagatac atagactcta acaagagttc ttttttattc ccaaaacgac
     3901 aaacaaacga ggtggtggtg tgcaagatgt gaaagaaaaa gtaaaacaag attacctaaa
     3961 aggtatgaaa caaaaggaaa tatcagcaaa gtatgacatt agtttaaata ctttaaagtc
     4021 atggataaaa agatacaatt gggctaatga gaaaaagaag ggtgcaccta aaaataaaag
     4081 aggtgcaccc ataggtaata aaaatgccac tggtcctcct ggaaataaaa atgctgaaaa
     4141 gtttggtttc ttctcaaaat atctacctga agaaactagg gaattaatac aagaaatatc
     4201 tataaaagat aaatttgata ttctttggga gcagataaca atccaatacg cagcaataat
     4261 aagagcacaa aagataatgt atgttaaaga caaggaagaa atgattaaag aattaaagaa
     4321 acatgaaagt acagaaaatg gagagaagat agagtatgaa tttcaatttg catgggatag
     4381 gcaagcatct tttcttaatg cacagagtag agctatgagt gaacttagaa gtttaattaa
     4441 acagtatgat gaaatgattc ataaggattg gaatttggct acagaggagc agaaaaatag
     4501 agttgaaaag ttaaaatgtg aagttgataa cctaaagaaa agtgatactg gagatgattc
     4561 aaaaatttgg gttgaagcta tacaaaatat tgcaatgaaa cgtggtgtta acaatggata
     4621 aagctttatt gacactatta gattgttatt gggataatcc tgtttggttt gcagaggata
     4681 tgttaaattt taaagctgac aagtggcaat ctgatgttct gatggcttta gctcaaaccc
     4741 caaaagtatc tattagaagt ggtcaaggag taggtaaaac tggattagaa agcattgcaa
     4801 ctgtatggta tttaagcact agaccttttc cgaaagtagt tgctacagct ccaacacgac
     4861 aacaattata tgacgtacta tgggctgaaa tagctaaatg gctaagtaat agcaaggttg
     4921 agaagctact tgagtggact aaaacaaaag tgtatatgaa aggctttgaa gaaagatggt
     4981 gggctacagc tagaacagca gtaaagcccg agaatatgca aggttttcat gaagattata
     5041 tgttatttgt tgttgatgaa gcttcgggag ttgctgaccc cattatggaa gctatattgg
     5101 gaacattatc aggtgcagaa aataagcttc ttttatgcgg aaacccaact agaacgagtg
     5161 gaacgtttta cgatagccat aatagagaca gagatttata taaaacattt aaagtatctt
     5221 ctttagacag ccctagaaca tcaaaagata atattgaaat gctaaaaaga aagtaccatg
     5281 aaggttctga cccttggcgt gtcagagtac ttggagagtt tccaaaaggt gaaagtgatt
     5341 ctttaatatc tttagaagct gttgaaacaa gcacaataag agaagtgaat atatctaatg
     5401 actatatatt aaatataggg gcggatatag caagatatgg tgatgatgaa accataatag
     5461 ctccaagaat aggtgggaaa gtatttgatt tattaactta ttcaaaaaaa gatacaatgg
     5521 aaacagtagg aaatatatta agagcagttg ataaatttaa aaatatgtat catcaaatta
     5581 acagagtaaa aataaaaacg gatgatgatg gcttaggtgc aggtgtaaca gatagattaa
     5641 aagaagttat aagacatgaa agacttaaat atgaagttat acctattcaa aatggttcta
     5701 gtgctataga aaaagataag tactataata aagcttctga aatgtgggat aacatgaggg
     5761 aggaattaga tgcaaattta agtagtttta tacaaaataa agaagctata atacagcttc
     5821 ctaatgatga taaacttatt aaacaactat caaatagaaa atatacagta gattcaaaag
     5881 ggaaaataca aatagaaagt aaaaaggaaa tgaaaaaaag aattggagaa tcacccgata
     5941 gagctgatgc agtaatatat tcgtttgcag aaaataacaa tactgattta tctttactga
     6001 aagggggtag tgtatgggga taatatctta tgtaaaaaag ctatttaaaa gacctgcagg
     6061 agagattatg cgtatgtcta gtggaaacat tggcgtatat aaattagacg attctagagt
     6121 tgattatgag ttagcaagag aactgtatca aaataaaaat gctaagtaca agctaggttc
     6181 tagttttgtt agaccgattg tcaattcaac aactggtttt atgggtgtac ctcattttca
     6241 aatagaagat gaagaagctc aatatatatt agatgaattt gttttagata acacatctaa
     6301 aatgttaaaa acacatacag atagtttaaa gcaaggtgat tgttatattt ggataactag
     6361 agaagaaaga gaaaatcctt tatatcccga taaaaaagtt agattaatat ataacttcat
     6421 atcacccgaa gaagtgaaag aaataatatt agaccctaca acaaaagagc ctatagctta
     6481 tatattagaa agtcaaaatg aatggactga cttaggagaa aacaagagaa aggctaaggt
     6541 aaaacaaata ataactgctg aaagtagatt tgttgaggtt gaaggtgata agatagaagg
     6601 tttagaagaa ggggaaacgc ctaatgtatg gggttttata ccaataatac attttaaaaa
     6661 tgaagctgat gaaacattga aatatgggca aagtgatata gaaccaatag aacctctttt
     6721 aaaagcttat catgatgtta tgttacatgc gttaaaaggt agcaaaatgc actctactcc
     6781 aaaactaaag ttgaaattaa ctgatgttgc aagtttttta gcacacaatt ttggtgttga
     6841 agacccagtt aaatttgcca aagaaggtgg aaagataaat cttgatgggc atgaaatact
     6901 attcttaaac aaagatgaag aagctgagtt tgtagaagta aaatcagcca taggtgatgc
     6961 taaggagctt ttaaagcttc ttttttattg catagtagat gtatctgaaa cacccgagtt
     7021 tatatttgga gtacatacac ctagtgcttt agcttctgta aaagaacaaa tgcctattat
     7081 ggtaaataag ataagaagaa aaagagaaca atttacaaat agctggcaat tacttgcaag
     7141 aatggtttta ataatgagtt ctaattctag tggtatgaaa tattcatctt atgatgtgac
     7201 tataggttgg gatgaagtaa atccacgaga tgataaagaa ttagctgaaa cactagaaaa
     7261 agtatgtagt gcattagata aagctttaga gggtggattt attagtgaag aatcaacagt
     7321 aaacttttta gcacagtata tagatacaat gagcaattat ataagtgatg accctgaaat
     7381 agttggagaa agagaaaaga taataaaaac caagatgtta aaatacagat tagatgactc
     7441 tcaaggttta aatgatgagt caaatgaaat tgagaaggaa ataaataaaa taaaggataa
     7501 taatggcaat ggataaaagt acttcggaat taataactgt tgcaggggag tacaagaaat
     7561 gggcattaga agctagaaaa aaatttatag atttaaggct caagcaagat gatgaaataa
     7621 gaacaatgta tattaacata acaagaaata ttacaaaaga aataagaaaa ggaaatcttt
     7681 cagactttaa caaagttagg ttaaaacaga tactaaaaca attaacacaa gaaataaaaa
     7741 tattaaatga acaactagta tttaattttg atgaatactt aaataaaaat gttgaaacag
     7801 ctactagtta ctctaaaaat attttaatta atgcagttga gacagctcaa ataactaaag
     7861 taactaaaac tatgatacaa aaagctttct atgatattaa tataagaact gtagaagctt
     7921 attatacaag ggttaaggat ggtttatttt tatctgatag aatttggtct aagtgtaaga
     7981 agtacagaga agatatgaaa gttatattac aaacagcagt aacagaaggt caagactgtg
     8041 ttaaaacagc taagatgtta gacaaatatg ttttaaaagg taagaaaact ttagttgatg
     8101 aatatccaaa tatgataaaa agaataggaa atagagtacc tcaaaatata agttatgaag
     8161 ctttaagatt ggcaagaact gaaatgacat cagcttatgg tgatggggtt ttagcttctg
     8221 caatgattaa ccctgcaacc ataggtattc agtttatgtt gtccatggca catcctcaca
     8281 cagatatatg tgacgaaata tgtggagagg ataattttgg tttgggtaaa ggtgtttatc
     8341 ctataaatga agctcctgta tatccattcc accctcattg tttgtgtatt atgcttactg
     8401 tagttcaacc attagatata ttagttggaa ggttgaaaaa ttggattaaa aatcctatga
     8461 atgatgtacc tcttgaaatg tggtatcaag aggtgtatgg aaatttgaat ttttaaattg
     8521 aaaggtggtg attaaatgaa tgtaataact ggagaaatgg actcaatgaa tgcgttaata
     8581 tctagtataa aaccttctga tattccttta gctaaagata tagacataga agctttaaaa
     8641 tctatagatg atgaccctct tgaggtagtt gttgagatac cagctacaaa atctaaaagg
     8701 ggatggaatt atactgctaa aagcttgaaa gatattgtag attacactaa tgaaaatact
     8761 cttaatggct ttttaggaca tcaaaaagct gaaaatatat caactgaatt tgcactacct
     8821 gtaacgcatt ggataggtgc agaaatgaaa ggggataaag cttatttcag agggctgatt
     8881 gatgctgatg caacaaattt aaaaagatgg attagaacta aaaggataaa agaagttagt
     8941 atatttggtt atccaaaact taaaaagagt gctaaaggcg aaatgaatgt tataggatat
     9001 gagccactat ctattgattg gactcctcta catagaccag gtatgccaac aagtattgta
     9061 ggtatggaaa tgagtcctaa tggcgaacag ttagatggaa cttttgaagc tttaagaata
     9121 gatttaagag aagctttaaa agctaagttt tctattaatg ataataattc atatctctat
     9181 atacaaaaca taagatatga taacaatact gtcatatatg agttggagca aaatggatta
     9241 tgcaagcttt atagtatacc atttactata gttgaaaata aaataaatct aggtgaagaa
     9301 attgaagtaa taaagaaaat aagctatgaa gctaaaggag aaatgaaagg agaggaaaac
     9361 aaattggaag gaaaagagtt aataaaaaat gtcaaaggat tactgcaaac tggtgaaata
     9421 tcatattcag aggtcataca aggaataggc ttaactaagg aaattgtgac aggagagatg
     9481 gaagatgtaa aaagttcatt aaaagcagaa aaagaattaa gagaagtgaa aaaagtactt
     9541 ggaatagtag gagagatgga cacagttgaa gtggcaaaaa aggcttcaaa agctttagaa
     9601 aatgagaaaa aggaagcttg gaactgtata gttaataaag taattaaaga taaagtgtca
     9661 ggtgaaatag ctcaaacatt agttaagaaa atgttaaatg ttgaggaagg ctcaagtgaa
     9721 gaagtaataa caggagaaat agaaaatata ttaaatgatg agtttgtaaa aaatacaatg
     9781 tctaatatgt ataaagataa tccaacaaca acaggattat taaactctag caataatgga
     9841 agtttaacaa ctaagaaaaa tagaatataa aggagtgatg tttatatggc atttaaaggt
     9901 caaccaacgc caagcacaat aacacagata acaagagcaa aaataagtga tgggaaatct
     9961 gtaagagtta ttctttcaga aggtgaaagc actaaaacac aacaatttta tcttataaat
    10021 ggattctttg gagtcgctat gcaagacgga gaaaaaggcg atgaagttac tttgcaaata
    10081 gagcaagctg aatacgaaac ggataatatt gttacatcag aagcttttga ggcagggaaa
    10141 ttgatttatt gggataatac agctaagaaa tttactacta catctgcaag taataggcta
    10201 gttggtagag taacagatgg gaaagacagt aataatgtaa tttggtttat attattacct
    10261 caacaataga aaaggagtga taaatatatg gcatttaaag taattagtca ggaaaatttg
    10321 ctggaacaaa aaagaaaaga aactttacaa gaagatatac catttatagt aaatggtgaa
    10381 atggaatatg taacaaagaa aatatcaaat ggagaaatgg aaaccttgga gttaaataag
    10441 ccacttggtg aaatgatgac ttttagctcg acttcaaatt taaaagagtt attaagaaaa
    10501 gttgtattag atgttgaact aggcagagag caagtacaac tattatataa accaatctat
    10561 gacagtatag cagattctaa tttaccacaa gttatggatg ctaagtgggc tttacaaggt
    10621 aactgtgtat tcctagagca tatagaaggt gaagaaatta aattcggtac aataaatgca
    10681 gaaaatggtc cagttgcaag gatacaaact tatgcaactg gttttgagta tacaaaagaa
    10741 atgaaggatt ttaaccaaac atttagtgtt gaaatattaa ataaatcaat tggtgagagt
    10801 tacaatgcct tgttaaacca catacatcta agcccaataa taaattttaa ttataaagct
    10861 tctaataaga cagcttttaa aggtgaaact aatgacccaa tatggctagg aatttggaga
    10921 acattaacac aagcacaaaa agatacagtt atagcaaaaa gacaaggtaa tatattaatg
    10981 gcttctagtg ctgaccaaat tgaaatagaa atggcgttaa atggaggaca tttattaaac
    11041 ggaagcatgt atccatctat aaaaaatata tcaacagtaa tttattatga tgggtgggag
    11101 gttactgttg gtaaaaaaac atattcttac aaaggtgtta caccaggcaa aggatatttg
    11161 ataagaccta agcgaggatt taaagagtta ataaagagag atttaacaac agaggttgga
    11221 aatgctgatt taagtaagtt agtagaaaat caaattgtag gtcattgtta tagaggtgct
    11281 tttgcagcag tagaagaaaa tgtacaagaa ataagtttta gataaaacac tcataagagt
    11341 gttattttta tgaggtgata atatatgaca ccaactagta atttaataga aaaattaaga
    11401 ctattattaa atgataaaga taaaaaatca tttacagatg aagaattaaa cttgttttta
    11461 gaagaagcag actgtattta ctgtgcagct tctcaaggat gggtattaaa atctttacaa
    11521 tatgaaaata cagtagggga aatgtatgag tataaagtgg gtcaagaaac atataaaagc
    11581 tctagtataa aggacctagt atctgtagct tatcaaaatg cagagaaatt caaggatatg
    11641 tgtactaaca aaaaagaaaa gggaagtttt atgttaggaa ttagcacaga atttgaaata
    11701 tgataaatat tgatagaaga agaaaagaca taataagaac tattaatatg aaccctacca
    11761 atattactat aactaatatt aaaaaaactg aaatagatgg agcttttgaa gaaactgaaa
    11821 cagagataaa atgtgttgtt agaatattta atgaaaagac agcagagaaa caaatatcaa
    11881 gtgaaaagca aggtactttt agttctatta gaacatatgg aatgttagta agtgatgatg
    11941 ttgtcttaga tgttaacagt agagattctt tggagtttga gtgcatatat gggagaatga
    12001 aaatagttaa tgtatatcct caaattgtaa aaggagaact ttgtgggtat caatgttcac
    12061 ttgaaaggat tgattaaaat gagtgctttc acaaatgcaa taaatgatat aaatagaaaa
    12121 aaagcaggta tgtttgtact ttgtatgagt gcaagtgcaa tgctagaagg tgaagctaaa
    12181 gcaaatgcaa gttggacaga cagaacatca catgcaagac aaagtttaaa tgctaaaact
    12241 cttggaggag gaaataattt cattattaga ttatcgcatg gtgcagaata tggaggaata
    12301 cttgaagaag gttcaaaacc acatgttatt actccaaaat cagctcaagc tttatactgg
    12361 agaggtgctt cacatcctgt aaaatcagtt caacatcctg gtacaaaagc aatgcctatt
    12421 ataaaaccaa ctattgataa aaatataggc aaaataggta atatgatttt tagatattgg
    12481 agtgattaaa tgagggcagg aataagaaaa gctttaatag ataatataaa agaattgaaa
    12541 ggttgttatg aacctaatgt accaaacaaa gatactaaaa agccttatat ggtagttgta
    12601 caagggcaag acaatgacaa tggagaaacg ataggttttg aaagaagtat agaagtatgg
    12661 atttatgaag gtagaactac atttaagaag ttagataaat taactaaaca agttattgaa
    12721 atcttagaca tgaatactat agttgatgaa tctgaaaatg aagctttcac ttgcatttat
    12781 aaagggacaa gtgaaaatga tattgttgtt gaggaatggg atgctatagc aagaggtata
    12841 aggtttagtg taatagcttt agaagataaa gaagatgcaa ctagcgatag atgggtagaa
    12901 gctttatcaa agtacacaaa agatttatta gaaattgaga gttataaaga taattggaag
    12961 aaaaacttta tagctccatg cgcattatgg cgaactacaa atgttgaaaa caaaagaata
    13021 aattatcatt tgattgagat taccaaaact atgaaatgtc atgttgtaag taaaaataag
    13081 gatgaaatag ttaagtttct tgaagcatta gaaacaagtt taataataga taaaagagta
    13141 agacttagag aggataagaa tatgtattta actcttgtta gcgtagttga ggatagggaa
    13201 tcagacatgt ttacaacagg acaattaaca gttgtgttca aaatgatagg taagataaaa
    13261 agagaaggtc ctattatgga taaaatttat agtaatggaa atttaaaata ggaggtgtaa
    13321 gggttggctg aaacaattaa taaaaagact aatgtaagta agcaggaaga aaaatatttg
    13381 aaaaatgatt tcttaaaaaa tagtgaagca cttggctacg aaaaaatggt agttgcaggt
    13441 gctttattta attgtaagaa agaagaactt acaaaatcag agtttgagaa attaataaaa
    13501 gattttttag aaagagaggt gaagtaaaat ggcaactggt acatggaatg aaaaagaaaa
    13561 aaaggagata ccgggctttt ataacagatt caaaacacaa gcagaaaagt ctgcaaacac
    13621 aggattaaag ggtagattag caataccagt taaggctaat tggggagaag ttggcaaggt
    13681 tgtaacaata aaaaatgact tgagacagct taaaactttg tttggtgatg atatgaacta
    13741 ttcagcgtat aagttaggta aattagcttt attaggaaat gtaaaagagc tgttattata
    13801 taggcttgta gatggaaaac aaaagaaggg tacattaaca ctaaaagata ctacagaaaa
    13861 tagtgcaaaa gatgtaatta agttagaaac taagtatcca acagctagaa actttaatgt
    13921 aacaataaaa tccaatttag tagattcaga taaaaaggac tttatattct ttgaaaatac
    13981 taaacagtta tttagttcaa gtattaaagg cactatagat gaaatagtac tagaaataaa
    14041 ctcaaattta gataatgagt atgtaattgc aactaaagta gctgatagcg atacaattct
    14101 agcaaatgta gtaaatcaag ctttagaggg tgggaatgat ggttgcacat ctattactaa
    14161 tgagtcttat ctaaaagcac tagaagaatt tgaaagatat agttttgact cttttgtact
    14221 tgatggtgtg gctgatgaag cattgcagga aactacaaaa gcttgggtag ctaaaaataa
    14281 agaattagga aaagatatac tactttttct aggtggaaaa acagaggata atataaaaca
    14341 gataaatgat aaatcaaaaa gtttcaatga tgaaaatata gttaacgttg gaagctcagc
    14401 ttattatgaa aatataaaat atacacctag tgaagtagct gtttacattg ctgctctttc
    14461 tgtaagtaaa ggtataacgg gtagtatatg taatgcaaaa actatatttg aagaagtaga
    14521 accacgatta agtcaatcag aagttaaaga gtgtttgaaa agtggtacat tggtcttaga
    14581 ttttgatgat ggagatgtga ttatagttga tgatgtgaac acatttaaaa aatatgtaga
    14641 tgataaaaac gaagcaatgg gatatatctc taatatcatg tttattaata ctataaataa
    14701 agatacttca ttaaaaagaa aagagtttgt aggtaagata tttaatgatg caacaggtca
    14761 aacaactgtt atatgtgcat tgaagaaata ttttgaagaa ttgatgagtc aaggtattat
    14821 atcagaattt aatgttgata tagatacaga gcttcaagca actgccaaag cagatgaatt
    14881 ttactggaag tgggatgctg ttaaggttga tgtcatgaaa aaaatatatg gtactggata
    14941 cttaggataa aggaggttat agattatgta taatgatgat tatatagaag aagccagttt
    15001 tctgaatggt tctgatgtag ttatacttat tgatggtgta gaagagctat acatggaaga
    15061 aataaaagct gattttgagc aagatgagca aagtattaaa ctgttagggt gtcaaaatga
    15121 aatatcaagg gttggtacta ctaaaggttc attctccttg aatggatata agacagattc
    15181 aaaatttgca aaattaggat ttaggtcttt tgaaataata tataatttat ctaattctga
    15241 aacattagga tatgaaagta ttagattaaa gaattgtaga ttaaaaaaat tgcctcttat
    15301 aaattctaaa gctggtgaaa ttgtaaaaat agaagtagag ggaagtttta gaggatatga
    15361 tttgttaaat gaactttaaa acaaaataat gttgtacttt aaagctatga acaattaatt
    15421 ttgtttgtag ctttttaaaa ttaaaaatat tggaggaata gtatgtcaga gatatataaa
    15481 agagagttag aaaatgaagt agtagaagat aatgatttag atgaagaaat agaagaaaca
    15541 aatgaagata gattgaagat gaaagaggat gaaataatag caaagctatt ggaggattca
    15601 ccagttcctc agagaactgt gtttttagat agattaggaa taccaattac tttgaaagca
    15661 ttaacagaaa aagagattag taaaataaga aaagaatgca ctaaaattgt aaaggtccaa
    15721 gggcgaagag aagaaaaatt aaatgatgat gagtttactc tagcattaat agaaaaagga
    15781 actgtaaaac ctaatttttc taatcaaaaa ttacttaatg ctatgaaagt aacaaatgct
    15841 agagaattta taaagagaaa attcttagct ggagaattaa gtaaaataag tgaccaaata
    15901 ttagaattat ccggttttta tgatgaaata agtgatgatg atataaaaaa ctaataaaaa
    15961 tggggggacg actgactgtt ttaaataaca tctttgtcaa gcatcatgtt cccccagatg
    16021 tttatgttaa gaaaaattta atgtctcaac gcttaatgaa agtatttact caaaatgaaa
    16081 tagagcaaga gaataaagct atgaaaaaat aaatatctac agaaaggtag gtgagggaaa
    16141 tagctaaaaa ggaaatgtat catattgatg ttgtcataag tgcaaaaggc gacggagaaa
    16201 caaaaagcaa actaagtgct atggaaaaat acatgaagca gacagaaaaa aggatgcaaa
    16261 cacttaatag gataaaggtt aatcctgcta taaaagctac tgataaagct tcaagtgttg
    16321 taaatagagt taataacaat atgaataaag caaaaaaaac tgttacagct agaataaaag
    16381 ctacagataa cgcgagtcca gtagccaata gagctagcaa taatgttaat aaagctaaaa
    16441 agacagtaac agcaaggttg aaagctacag ataatgcgag ttctactgtt aacaaagtta
    16501 ataataagat aaaagaagtt gctaagcctg tacctcctgt aatcatacga ggacaagatg
    16561 aatctagttc tataatagat aaagtaaaag ctaagattca gaatctaaaa gctgatacta
    16621 tcataaaaat aaaatcacaa gctgatgaag ctataaatac tatttctcga actaaaaata
    16681 aattacaaga atttgtgagt aagagatatg aagcagcagt taagattaga gatgaagcta
    16741 gttcagcact tagagggctt acaggaaaaa tagattcttt tgtaagtgga gctattagta
    16801 aattcgctag actggctact accgcaggag ctttaatagg tggaattggt gtaggttctg
    16861 ctgtaaaagg atttgctact tttgaacaaa gtatgaagaa tacacaagcc gtaagtggag
    16921 caacaggaaa agaaatggaa gctttaactg caaaagctag acagcttggg agagaaacta
    16981 gttttacagc taaagatgca ggagacgcaa tgtattacat gggtatggca ggatggaagt
    17041 ccgagcaaat gataaaagca attcctgacg ttcttaactt ggcagcagca ggaggaacag
    17101 acttagcgtt aacgagtgac attgtgactg atggactaac tgcattagga atgactgcaa
    17161 atgacacaac tgaatttgtt gatgtgatgg cagcaacaat aactaattca aatacaagtg
    17221 ttgagttaat gggtgaaaca tttaaatacg taggctctat gggcggagca ttaggagtat
    17281 ctatgaaaga tttatctctt gcgacaggtc taatggctag tgcaagtgtc aagggaagta
    17341 tggcaggtac ttcgttaaga ggaggtctag ttagattaat aaagccacca gaagaagccg
    17401 catctgcaat taaaaaatat ggaatagaat taaagaaaaa taaaaatgga agtttagact
    17461 tggcaggaac aataggaagt cttagagaga aattaggagg attaaaagat gttgaaaaag
    17521 gtgttgcaat atcctctata tttggtcgta ctgcaatggc aggttgggcg gctgtagtaa
    17581 atgctagtga aagtgacttt aataagttaa ctacagctat tgcagaaagt gaaggagaag
    17641 ctaagagaat tgctgatatg aagttagata ccttatcagg acaatttgaa attttaaaaa
    17701 gtgctattga tgatgtaagg ataagtgtag gtcaaagact aggacctatg acaagaggat
    17761 ttgtagaaga tttaattaag aaaatgccac aaatcggtga tgctatagtt ggagtagtag
    17821 aaaagtttgt taataatttc gataaaataa aagcaggatt tcaagtatta ttgcctgcaa
    17881 ttggttccgt tatagcctct gttatggtac ttaaagccac atttgctttt ggaggagcca
    17941 ttaaaagttt aagtttactt gcaagtactt ttggtacggc taaattagtt gccttcggat
    18001 taacagttgg aataggggct attgttatag cttttgcagg aatgactgtt gctatttcta
    18061 ataataaaac tgctatgatg gacttacaaa cacgttttgg ttcgtttggt gagtatgtta
    18121 ctacaataat ggagactgtt ggtggagtca taaagcttac gttaggtaac ttgcttataa
    18181 tgttaggtgg aattggaaaa ggaataggaa tacttttatc tgataagagc tgggatgaaa
    18241 aagcttcatc acttaaaaat ctatttggca aaacaacagc agaaattaaa acaaatacaa
    18301 aagaagcttt atcagatata aatggagaaa cttctaatgc aacagctcta ttaaaaaagt
    18361 ctacttcaaa agagttacaa ggtgttacta aagctttctc aacagcattt gaccaatcta
    18421 aaaatgtaac agagaagaaa tctagcgata tagctagagc attaacaaat ggattaaagg
    18481 gtttagatga ccaatctctt actatgatga gaggtttgaa tgacaatatg gcgataattt
    18541 tatctggtgt gacagcagat atgaaaccta gtgacaaagt taataaaatc accaaaaatc
    18601 ttgatgatgc tttcaaagca ggtaaattaa gtgcacaaga atacagaagt agcatacaag
    18661 agactttaaa ttttataagc aagtatagtg cagattcttc aaataatttg aaacaaggta
    18721 tgagtgatgc tttcaatgcc ttcaaggaag ggacaaatat aggcggatta aaagacggag
    18781 caacaggaat gttgaactct ttaaaagcaa ctggtccaca agctttagaa accttaaaag
    18841 gtttaggagg aaaagcaagt gagattttca aaggagtgga ttttaattct tctatagatg
    18901 cacagaaaac taaagtatta cagaatttaa atagtttagg attagaagga acacaggcta
    18961 tagatacttt aagaactatt ttctcacaag catcatctgc tttggatact acaaatttaa
    19021 agcaaggttt aagcaataca tttaactctt ttaaagaagg atttaatagt ggaggaataa
    19081 aaaacgctat aaatagtatg cttggaacta ttaaccaagc aggtccacaa atgcaacaag
    19141 cattaggcaa aatggatgga aagatggggc aagtatttgc taatgtagac tttagtactc
    19201 caatagaaac acaagctagt aaagttcttg aaaatttgaa taatctagga atagaaggac
    19261 caaaagcttt agaaactgta agaagtatat ttgctcaagc ttcaagtcaa atacaaggtt
    19321 cagcttctca aacggcacaa caagcgaatc aagaagtggc taatgcttta actcaaggta
    19381 acccaactgt acaacaagca gggcaacaat taggtacaga tttgaccaat ggcgtagtaa
    19441 atggagtaca ggcaggagtg ccagttgtac agcaaaaaag taatgaactt gctacagcaa
    19501 cacaaaacgg agtaacaaat gctgtaaata gtgcaactcc tcaaatagat aattcaaatc
    19561 ttacaagtgg tatagataca gcattcaatc aagctactgc aacagttcag caaggggcga
    19621 gtagtatgta taatggtgct aaacaaagtt ttacacaact agcacaaata ggtagagaag
    19681 caggttcaag tctttataat ggtgctacaa cttcttttaa tatgttagca acaaatgtta
    19741 gagttgcatg tagtagcatg tacaatggag ctagaacatc ttttacgagt ttaagcagtt
    19801 cagctataag tgccatttct gctatgtgta gttctgtagt aagccaagtt agtgccatgt
    19861 cgagtcaaat tatcagctat tggaatagtg taagagcaac agtttcagca ccaattacag
    19921 catcttttaa tgttaagact actcaaacaa cagttaaaag aaccgtaaat gaaagtagtg
    19981 gtggaatact gggtaatata ctagaccgtt ttgctgatgg tggagtagca acaagaactt
    20041 ctatctgcgg agagaatgga gctgaaatgg ttatccctct tacaaacact agaagaagta
    20101 gagcaataga attatatgaa caaacaggta aaatgttagg attaggttca actccttctc
    20161 aaaaagtagg taattctaat attgtaaata atgttagaca atttcctctt gcaaatgttt
    20221 tagatacaga taataaagaa tataaagaag ctacaccaaa cagtgttaat tcttctaata
    20281 atactataaa tttaggcgga atatctataa atgttcaaaa tagtgataac aaagaagaaa
    20341 tgatacaaga actattatct caagtagaaa gtggaataag agaagcatta caagatattg
    20401 gataatgtcg aattgttgct aaaaaaatct tcctcataaa tgatataata ttgttaaaat
    20461 atgcaaattt atgaggggga aattttatgt ggcagaaatt taaaagtatg aatggaattt
    20521 taaaaattat tattggaatt atagctataa ttcttttacc aataacattg gttttgttat
    20581 ctattgagtt tttagttaaa gctattggaa gtaagaaaaa agctaaaatt gcattaggtt
    20641 gcatattagt ttttttgaca acactttgta ctagaaatgt ttataatgta ctaagtatag
    20701 agaaagaaga gcatattcct gtgaagcaag tttcttataa tgataaagat aagggaaaat
    20761 ccgagaataa tattcaagaa caacaacaaa agaaagaaga atcaaaaaaa ttggaagaac
    20821 aaaaaaagaa tgaagaaaaa gagcaaaaca atgaaccaac aaagtttttt actgatgtat
    20881 atttaaaaaa taaaaattgt gttaatatga catcttatga aggtataaag tcaaacctag
    20941 atatattcgg ttataaatat gaagttatag agccgaatga aactacatta agacagatta
    21001 atgtatatga taaccaaagt aatgatacca ttcaatttca attttatgaa aatgatactg
    21061 gagaagaagt tgtaacagtt atgcaataca ataaaaaaga tgaacccgac aaatctgttg
    21121 caataagtga tggtttccat acacaaccac caaaatttca gaatcatatt aataatacaa
    21181 ataaggaagt taatagcata gaagaacaaa taaattttat gtttaattaa tatttgtata
    21241 atactttaaa gctcttacag tttgtaggag ctttttattg taaaaaagag gtgataactt
    21301 ggtaatagat atttatctaa aaaatgaaaa agaaaaaata gattttcatt ttccagtaaa
    21361 tccacaagat tctttatcta taaaaaaaga aaaaaggttt gaaactgtag atatagtaaa
    21421 cttaggtgaa tttgatataa aaaaagaagg ggagaagata agagaaatat catttaaaac
    21481 atttctgcct aacttatatg acgcttctta ttgcagatac agcgagttaa aaaatccaat
    21541 cgaagtagtg gcaacgcttg aaaaatgggt agaccaagcc gaacctttaa gacttattat
    21601 aactggtttt ggctacaatg gattagtcac aatatctagt tttagcaata ctcaaacagc
    21661 aggaagagaa gaagatagag acattgagat aacatttaga acttacagag aactgaagat
    21721 agagacatta aaaaaagata caaaaagtaa tactaaaaca gatttaaaag ataatagacc
    21781 taatacccaa actaaatcta aaatatatac tgttaaagca agtgatacat tatataagat
    21841 agctaaaaat cttttaggta agggttcaag gtggccagag atttataata tacccgaaaa
    21901 caaaaaagtc attggtaaaa atcctaatat aattaaaaaa ggtcaaaagt tggtgatacc
    21961 tagtaaatga aaataatatt aaacgggaaa tatgatattg caaatttcaa tgaaggaata
    22021 actctttcag aagctataga cggagttgca tataagatgg atgtatcatt agtagaacct
    22081 aaacaactta aagatataaa tattaaaaaa ggtgataaaa taattctaat tgatatagca
    22141 tatgagagta aaaaagaaga gacaatattt gatggagttg tatgggaaac tagaaggagt
    22201 gaaaagagta agaaactaac attgtcttgc agagaaagaa cagtttacat ggaagaatca
    22261 gaggaacaat atcagtttaa agaaaataca gcaacacaga gaattgaata ttactgtaag
    22321 caatggaata taccctatta caacttagct aatacaggga agaaacttgc taaagtaata
    22381 cataagacaa atatactaga tatgataaaa aaggacttaa aagaaacagc aacaaaaggt
    22441 ggagacttat ttagagtaag gatggataat aaattaaaat tattcaagct tggtactaat
    22501 gcaaatgtat ataaattaga tagtatatta gaagatgcta actttacaag tagttttaat
    22561 gatgcagtaa caagtgtaaa agttttaggt aagagtaaag acgaaaatac aaaagcgcct
    22621 ataattggga catataaaaa agatgctgat aagtttggaa cactacaaaa gattaaacaa
    22681 gatgaaaaga taaaaaatgc taaagaagct aagaaagcag cagaagcaat gttcaatagt
    22741 ggagaggaaa caataagtgt agattgtgca gtagatataa atagaataag agcaggtgac
    22801 aaagtaagtt taaaaagtaa agaatattat gttatagatg tcactcatac actagattct
    22861 agaccgaaaa tgaagctgaa tatagggtct ttagaatata taaggaggaa attttataat
    22921 aatgactgat gctagattta atggaattgc tagaatattg aaagaaaata tgaataaaag
    22981 tgtagcaaat ggcacttttg gaatgggttg tgaacttgca gaaataacag caaatggatt
    23041 aaaagtaaat ggctataaag atgaaataca ggactatcta gtattagaga atttaacatt
    23101 aaaagaagat tattttactt tttcagatga agctttaagt ggagaatata gacataagca
    23161 taaaatagaa actccaaagg aattgaagcc actacgtata ggcgataatg tgctagtagc
    23221 tgttatggga gctgaatttg tagtaattgg gagggttgta aatgccaaac ctatttcctc
    23281 aaagtgaaac ttttgaaact gtagaattaa aaaataatga tgaaaatgaa ttggacctaa
    23341 agggttcttt tttatttgat tttgaaaaag gtgaatttgt taaaaacgca gatggaacac
    23401 taaaaaaatg tgataaggtg caggcgtaca aacaatggtg tcaaaaggct atattaacac
    23461 ctagatacaa aaaagcagct tatacaaaca tttatggaag tgaaataaaa gacttaatag
    23521 ctagtaactt atctcaaagt gcaaaagagc ttgaaataac tagattaata aaagaaacta
    23581 ttttggttca tccttacaca aaagaagtag gagagtttag ctttaattgg ttggagaata
    23641 gcaggttagt agagtatgaa tttgatgtac taacaataga tgatgaaaat atagtaattg
    23701 atggcaatat aaaaaggtag gtgattatat ggaaagagag ctacctatac cagtattttt
    23761 aacagaagat gaggactctg tacatgaaag gatgttaagc aactttcaag atgtttctac
    23821 attagaaggt gacttcatct atgatgcaac aagacctaca gcagagcaga tagctgaatt
    23881 aaaacaacta ggattacaaa ataatttaaa gatagcattt cctcagactt cttatggaac
    23941 ttatttagag tggcttggtg aatgtaaagg agtatttaaa aatcaaccaa ctaaggctac
    24001 tggagttatt acatttacag gtgtacaagg aactatcatt acaaaaggaa ctatagtaac
    24061 tactattgca actgatgaaa aacagagcat agaatttgag cttcttgaaa ccaaaactat
    24121 aggagaaaat gaaacagtag atattaaagc agaaagtagg attgtaggaa ctatagggaa
    24181 tgtgtctaaa ggtagtatat ccgttttact aggttctatt aatggtgtta aatcagttac
    24241 taataaagat ttcaaaggtg gaacagatat agaagatgaa gaacatttta gagaaagagt
    24301 ccttgtagca gagcaagaag acaaacttag tggagctagt tcagattata taagatgggc
    24361 taaagaagta gatggagttg gatatgctta tgtagttccc gaatggaatg gagcggggac
    24421 agtaaaagta ttaatactag ataaaaatag aaaagcagca acacaagagt taatagacaa
    24481 ggttcaagaa tatatatatc cattaaatat atcagaagga gaaaatagag atgggaaagc
    24541 tcctatcggt gcattagtta cagttgtaac acctgacaca ttacttatta atgtaaaagc
    24601 tagttttata tttagtaaca gttttaatga agaaacagta ttaaacaatc taaaaactaa
    24661 gatagataaa tatttagata agattgattt agggggaaca gtttcataca atgctataca
    24721 agcgatagta ggttctatga tgttgacaga tgaaggtata caagactttt ctaatcttac
    24781 tataaatgat gcaaaagaaa atataaaatt gcaagaccaa gtggtcggaa taggggaaat
    24841 agttaacgag gtggttggat gataacttct aaaaaaggta aagaaatgct tctaacatta
    24901 tctcctatct atgaacaatc tttagttatg aactctatat atgaagctat aggaagcgaa
    24961 tttgataatc tagaattatt aaataaagaa atagagttac aattatttcc tcaaactgct
    25021 acatggggac ttgaattttg ggaaaatagg gtaggtttat ctactaatat agatgaagat
    25081 atagaagcta gaagaagaaa agtcattgct aagcttcaaa tgaaatatat tgttaatcct
    25141 aacagactag caactataat aaaaagctat actggtacag atgtatatat aaaagaaaat
    25201 atagctccat acacttttaa agtaactgct aatgttgatg atgttattaa ttatgaagat
    25261 ttcaaatata taacaaataa aacgaaaccc tctcatcttc attggatgcc ttcttttgca
    25321 ctcaaattta cagatataga aaaatttgag gtaaagatga ttaatcgaat atttatagat
    25381 tttagaggga atataagtaa tttcttagat ggcatgtggt tattaaatgg tagtaaaaac
    25441 ttaagtgctt atatacttta taatgagcca ataagcttaa acatgaaaaa taagttattt
    25501 gtaaaagaaa gtgaagtatt tacaaatctt aaagtaatca ttaaaaagaa tttatattat
    25561 ttaaatgggc tagaaatgtt aaatggaaat aaactgctca atgcagaatt aagagaggaa
    25621 gtgttataag tatggcaaat gcagtaacaa cagacattgc aagacaaaaa atgtgtaaag
    25681 ctcgagcggg tgatataaca ttacctacta tagttagcat ggcatttgga gatggaggaa
    25741 ttgggaatga tggaactata atagctccac tttcaagtga tatagcgtta aaaaatgagg
    25801 tgtttagaaa agatatagaa aattatgtat atccaatccc aactacttgc agatattcaa
    25861 ccactctatt aaaaaatgaa gcagaaggaa aaaacataaa tgaaataggt ttaatagatt
    25921 ctgatggcga tttaattgca attaaatctt ttggaaataa gtataaagac agtgatatgg
    25981 aaatggtttt tcagatagat gacgagttct aggaggtgaa taaatgccta atgaattaga
    26041 ttttaataat gagattgaag aatatttaat aactacacca gctcatgcaa atgagtttaa
    26101 taatcgacaa caaaaattgt tagacaatga taaatattta aataataaaa ttgatacaac
    26161 taaaacagag ttaaatacta gaattgacac agaaaatgag aaacaaaata ttaaaattga
    26221 ccaattaatc gcaggtggtt ctaatgtggc atctactcaa acaataacaa ttgacgattg
    26281 ggttgaggat gcagaaagtg gattcaaatc aactgtaaca catagtttat taacacagag
    26341 aatagttgta aatattatag atgctactac aaaagaaaat gtagttacaa actttaaaat
    26401 tatcgatgat aattctatag aaattagaag tgaaacaagg tcagaattaa acgtttatgt
    26461 gataaatgga aatgcagaaa ctcattttat taatgcaact gtagatgata acagagtatc
    26521 tgaaatgact acttattcgt ctaagaaaat cgaagataga tttgttaata tagaagaaaa
    26581 attgagtggg agtttatcta acattgcaac aagtgtaaat gagttgataa cttattgtta
    26641 aaggagagtg aaaaaatgca gactgaatgg acttttgggt ataatggttc gccacaaagt
    26701 gttatattga aacctggtaa atataaattt gaatgttggg gttcttctgg aggtatcaac
    26761 aattcttctt ggtatactga tgctaaaggt ggatattcca agggtgaaat tacattaaaa
    26821 aaacaaacta cattatatat ttatgtcggc gaaagtggtt atgcttcttc atctacagct
    26881 aataacacta aaagtggttt taatggtggt ggcaaaggtt atttaaatca acaggttatg
    26941 ggtacttatt attctatgta tggtggtgga gctactgata taaggctcgt tggtggtgct
    27001 tgggataatg agcaaggttt gctatctcgt ataattgtcg caggtggtgg cggtggttca
    27061 tattctcctt atactggtgg tgcaggagga ggattag
//
LOCUS       DAGSWI010000049        26850 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.049, whole genome shotgun sequence.
ACCESSION   DAGSWI010000049 DAGSWI010000000
VERSION     DAGSWI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26850
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.049"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(50..1579)
                     /gene="lysS"
                     /locus_tag="MJF80_001870"
     CDS             complement(50..1579)
                     /gene="lysS"
                     /locus_tag="MJF80_001870"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBZ0280533.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(1594..2073)
                     /gene="greA"
                     /locus_tag="MJF80_001871"
     CDS             complement(1594..2073)
                     /gene="greA"
                     /locus_tag="MJF80_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBZ0280534.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2182..3150)
                     /gene="dusB"
                     /locus_tag="MJF80_001872"
     CDS             complement(2182..3150)
                     /gene="dusB"
                     /locus_tag="MJF80_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBZ0280535.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3287..4057)
                     /locus_tag="MJF80_001873"
     CDS             complement(3287..4057)
                     /locus_tag="MJF80_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBZ0280536.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4076..4666)
                     /locus_tag="MJF80_001874"
     CDS             complement(4076..4666)
                     /locus_tag="MJF80_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBZ0280537.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(4850..5818)
                     /locus_tag="MJF80_001875"
     CDS             complement(4850..5818)
                     /locus_tag="MJF80_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBZ0280538.1"
                     /translation="MYNNILDIEGLKVGQIEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKRTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(5811..6788)
                     /locus_tag="MJF80_001876"
     CDS             complement(5811..6788)
                     /locus_tag="MJF80_001876"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBZ0280539.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTDFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWNSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7103..9073)
                     /gene="ftsH"
                     /locus_tag="MJF80_001877"
     CDS             complement(7103..9073)
                     /gene="ftsH"
                     /locus_tag="MJF80_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBZ0280540.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9107..10495)
                     /gene="tilS"
                     /locus_tag="MJF80_001878"
     CDS             complement(9107..10495)
                     /gene="tilS"
                     /locus_tag="MJF80_001878"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBZ0280541.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(10678..11106)
                     /locus_tag="MJF80_001879"
     CDS             complement(10678..11106)
                     /locus_tag="MJF80_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBZ0280542.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEENIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11123..11929)
                     /locus_tag="MJF80_001880"
     CDS             complement(11123..11929)
                     /locus_tag="MJF80_001880"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBZ0280543.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12131..12616)
                     /locus_tag="MJF80_001881"
     CDS             complement(12131..12616)
                     /locus_tag="MJF80_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBZ0280544.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(12667..13326)
                     /gene="spoIIR"
                     /locus_tag="MJF80_001882"
     CDS             complement(12667..13326)
                     /gene="spoIIR"
                     /locus_tag="MJF80_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBZ0280545.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(13471..14151)
                     /locus_tag="MJF80_001883"
     CDS             complement(13471..14151)
                     /locus_tag="MJF80_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0280546.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14135..15025)
                     /locus_tag="MJF80_001884"
     CDS             complement(14135..15025)
                     /locus_tag="MJF80_001884"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBZ0280547.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15143..16693)
                     /locus_tag="MJF80_001885"
     CDS             complement(15143..16693)
                     /locus_tag="MJF80_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBZ0280548.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(16787..17053)
                     /locus_tag="MJF80_001886"
     CDS             complement(16787..17053)
                     /locus_tag="MJF80_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBZ0280549.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17201..18061)
                     /gene="yabG"
                     /locus_tag="MJF80_001887"
     CDS             complement(17201..18061)
                     /gene="yabG"
                     /locus_tag="MJF80_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBZ0280550.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18145..19083)
                     /locus_tag="MJF80_001888"
     CDS             complement(18145..19083)
                     /locus_tag="MJF80_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBZ0280551.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19193..19354)
                     /locus_tag="MJF80_001889"
     CDS             complement(19193..19354)
                     /locus_tag="MJF80_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280552.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19364..19861)
                     /locus_tag="MJF80_001890"
     CDS             complement(19364..19861)
                     /locus_tag="MJF80_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBZ0280553.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKTYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(19976..20686)
                     /locus_tag="MJF80_001891"
     CDS             complement(19976..20686)
                     /locus_tag="MJF80_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBZ0280554.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(20862..21392)
                     /locus_tag="MJF80_001892"
     CDS             complement(20862..21392)
                     /locus_tag="MJF80_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280555.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21409..21639)
                     /locus_tag="MJF80_001893"
     CDS             complement(21409..21639)
                     /locus_tag="MJF80_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280556.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(21654..23048)
                     /locus_tag="MJF80_001894"
     CDS             complement(21654..23048)
                     /locus_tag="MJF80_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBZ0280557.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23174..24427)
                     /locus_tag="MJF80_001895"
     CDS             complement(23174..24427)
                     /locus_tag="MJF80_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0280558.1"
                     /translation="MENKSNFKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(24478..25632)
                     /locus_tag="MJF80_001896"
     CDS             complement(24478..25632)
                     /locus_tag="MJF80_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBZ0280559.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26192..26761)
                     /locus_tag="MJF80_001897"
     CDS             complement(26192..26761)
                     /locus_tag="MJF80_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBZ0280560.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
BASE COUNT         8188 a         5180 c         2685 g        10797 t
ORIGIN      
        1 aatttaatag ctaaacttta tttaaaactt agtagaatgc taatatttct tagttttctt
       61 cttctttatt tggattgtta tcaataggtt tcatagtcgg gaataataat acatctctta
      121 tagatggaga gttagttaat aacataatta ctctatctat acctattcct agtcctcctg
      181 taggaggtag acctacctct aatgctttta agaaatcttc atccatttca aaagcttcat
      241 catcacctaa ttctcttttt cttaattggt ctataaatct tcctttttgg tcaatagggt
      301 cattaagttc agaaaatgca ttagctaact cccacttatt agcaaatgcc tcaaatctat
      361 cagttcttct tgggtcttca acatttctct tagataatgg agatacttca actggatgat
      421 gagttataaa tgttggttgg attaatttat cttctccaaa ttcttcaaag aaagcattta
      481 ttacttctcc tcttctcatt ccaggtttta tctctatacc tttttctcta gcaacttcta
      541 gtgcctcttc atctgtattt atagtagaaa aatctacacc agaatattct ttaacacaat
      601 cttccatact cattcttttc caaggtggtg taaagtttat ttctgttcct tgatagttaa
      661 ctatcatact tcctgtagca acttcagcca tatgtgcaat aacattttct gttatttcca
      721 tcatatcagt atagtctgca tatgcttgat ataattctat tgcagtatat tcaggattat
      781 gttttaaatc cataccttca tttctaaaca ttcttcccat ttcataaact ttatcaaatc
      841 caccaacaat aagtcttttt aaatacaatt cattcgctat tcttaaatac attggtatat
      901 gtaaagtatt atgattagtt ataaatggtc tagcatttgc cccacctgct atagtattta
      961 atattggagt ctcaacttct aaaaatcctc tatcatctaa gtatgctctt aatgctttta
     1021 atgcttttgt tctaattaaa aaagcatttt ttacttctgg atttactatt aagtcaacat
     1081 atctttgtct atatcttagt tcttggtctt ttaatccatg atatttttct ggtaaaactt
     1141 gtaaagattt acatagtaat actacagatt tagcctttac agatatttca ccttttttag
     1201 ttttaaagat ttctccttca attccaacta tatctccaat atcatatgtt gaaaatacac
     1261 tatattcttc ctctcctata gcatctagtc ttacataaga ttggattctt ccctcttggt
     1321 cttgaatatc tataaagcca gctttccctt gtattctctt gctcattata cgtcctgcta
     1381 tttttgtagt tttaccctca agagaatcaa agttatcttt tatatccata gaataatgag
     1441 ttctatcata tctactttct ttaaaaggat ctctacctga ctcttgtaat ttttttagtt
     1501 tatctcttct tacttgaagt acttcactaa ggtcttcttg aatttgcgct tcttcattgc
     1561 tttgttgatt atttttcatt tttttcctcc aaattatctt cttatttcta atatttcaaa
     1621 cttagcaact ccatcaggaa cttgcacttc cacaacttct ttcgcagctc tacctaaaag
     1681 tgctttccct acaggagatt cgtttgatat tttaccatca tatgggtctg cttctgtcga
     1741 accaactata gtatattcaa cctcttcatc aaattctaaa tccttaactt taactattga
     1801 tcctattgta acaacattaa ggtctatttt gctttcatct atgataacag cttttctaac
     1861 catattttct aattttaaaa ttctttcttc aacttgtgct tgttcctttt tagcttcatc
     1921 atactcagca ttttcagata aatcaccaaa agatatagca acttttattc tctctgcaac
     1981 ttcttttctc tttacaacct ttaagttttc taattcttct tctaatttat catatccctc
     2041 ttgtgtcaat aaaaactctt tattttcttc cattataaat acaactcctt cacaaatttc
     2101 ttaggaattt tcctaattat attttaaaaa ttttataatc tttttttaat ttatgcgcta
     2161 tattataagt cactattatt tttatgtcaa ggagtcttcc atatagtact ccaatttcga
     2221 cacaacttct tgatagtctt ctattttgtt aatttcatcc cttaattttg cagagtttct
     2281 aagtcctttt aaataccaag cgatgtgttt tctcatttcc cttacagcta catactctcc
     2341 atgttcttct actgcaagtt ttaaatgctt aatggcagta cttatttttt cgttaagagt
     2401 tggctctggc aaaatctcgc ctgtattcat ataatggttt attcttttaa atatccaagg
     2461 gttaccttga gcacccctac ctatcattat agcatcacaa ccagttttgt ctaacatatt
     2521 tattgaatct tctattgtaa atacatcacc atttcctata acaggtatac tcaaattttt
     2581 cttaatctct gcaattatat cccagtctgc tttgcctgta taaaactgtt ctctagttct
     2641 tccatgtatt gctaaagcac ttattccaca atcctctgct atctttgcta tttcaacagc
     2701 atttacacta ttatcatccc agccttttcg aatctttaaa gtcactggct tttttgagtt
     2761 cttcactact gccctcaata cctcttctgc aagcttagga tttttcataa gtgctgaacc
     2821 atctccattt ttaactactt ttggagctgg acaccccata tttatatcga gaatctcatt
     2881 tgaataatca ttcattatct ctgctgctct tcccataaac tctggttctg aaccgaatat
     2941 ctgaactgct acaggatgtt cttcctcttc tatcttaagc atttttttag tattttcatc
     3001 atcatagcaa agtgccttac catttatcat ttctgtatat aaaagcccac atccctgttc
     3061 tttacaaatt aatctaaatg gaaggtctgt tacacctgcc ataggtgata gaaaaacttt
     3121 atttttcaat tctaaatttc ctattttcat tgtttttctc ctatttttgc ctttattttg
     3181 tgatttaact ttaaattctt atttcctcat tataatatac cacatatagc tagaaatcta
     3241 tcataaaaag ggaattggat atataaccca attccctgta tattaattat tcttggtttt
     3301 tttcatatat tattctcaat ccttctagtg ttaaaaaacc atctacataa tctatacttt
     3361 tcgtctctga agcaatcagt ttagctaatc cacctgtagc tataacctta acatcatcac
     3421 aattcaattc tttcttcatc atacttatta ttttgtcaac taaaccaaca tatccataaa
     3481 ttattccaga ttgcatggct gatacagtac tcttacaaat agtcatacct ggcttagcta
     3541 attctactct aggtaattta gacgcacttt gaaataacgc ctcactagat atttttattc
     3601 ctggtgctat tgttccaccc aaatattcac ctttttcaga gatagcacaa aatgtagttg
     3661 ctgttccaaa atcaacaagt atacttggtg ctccatactt ttctatccct gctacagcat
     3721 taactattct atctgcccca acttgttttg gattatcata ttttatattt agacctgttt
     3781 ttatgcctgg acctactatt aatggttgtt ttttacagta ctttatacaa aagttttcaa
     3841 gagaatgcat aacattcgga actacagatg atattataac atcatcaata tcacttatat
     3901 tcacattatc ataatcaaat aggttactta tcaggattcc atattcatca gacgtttttt
     3961 ccctatctgt tttaattctc cagtaattaa ctaatttgtc acctttatat atacctaaaa
     4021 ccatattagt atttccaaca tcaaatacta gaagcatatg tcattaacct tctttctatt
     4081 ttttgctttt cttaagtaca gtggcaacag cagctactac taaagcccct actattgctt
     4141 ctggcacccc atttgctgcg gcaagagcca ttattacttt tcctgctgaa ccacttttac
     4201 ccattgcatg aagatacttg tcagcatata agacatatat cattcctaaa actccagctg
     4261 tatttaacag agaacctatt gcccctgtaa ttaaacctga tatgtatacc ttatttgtag
     4321 ccttaattat taactgatat acataataag ctaatacacc tatcataact cttggtaata
     4381 ttgaaactaa tggatttata aaaacaaaag atgttactgt tgggtttgtt atagcattta
     4441 agagacttga tatcccaaat ataaatccta ttgctgcccc aactattggt ccttccataa
     4501 tagctccaat aataactggt atgtgcatta tagttgcttt tgtaggtcca actggaataa
     4561 atcccaatgg agtcatagat agcataatag aaatagctga tagtactcct attatagtca
     4621 tctttcttac atttactttt cttactgatg cacttgtgtt catcataaaa aatacacctc
     4681 cgttccagct cattaagatg ccggactact taatttttta atttttttcg tttttttctt
     4741 ataactatga gtcaagctac cattgtagga tgcccgctct aacaagaata attttatcaa
     4801 gatgagttgc cattgtcaac aatttgacaa gaaaaatatg ctaatttcat catatttctc
     4861 gtttttttac tatatcttta tatgccatta tattttctac actatccgct ttttttactg
     4921 catttattat acttttttcc acaacctcta cagccaatga acctactaat gttatatctg
     4981 cttctatttc tcctgttgat attgtgaata ttgtatctcc atcatgaggt gtatggatag
     5041 gaaaaataga cttagcatat ccatcatgtc ccatttgaga tatcttttta catccagctt
     5101 tggttaactt agcatttgtt gcaattatcc caattgtagt attatctaca ctaaatccac
     5161 ctttactttc accttgtttc attaatttat atgtatcaat tgtttctgtc cttttatcat
     5221 ttaaagcacc agctataacc ttaccatttt cataaacatc tccaagagca tttacagcaa
     5281 ttaatgcaga tacaattaga ccattatcta attttataga atagctacct attccgcctt
     5341 tcatagcata ttcctgacct cttatctttc caacagtggc tccacaacct gctccataat
     5401 ttccttgatt taaaatatca gtatttgcaa cttcacaagc tctataaccc atctttaaat
     5461 ctggtctaca tttaaagctc cctactccta aatcaaataa aactgctcct acaactatag
     5521 gcactttacc tacacctaca tcaaagccaa ttcctttttc ttctaagtat ctacttacac
     5581 cacaagtgga ttcaagacca tatgcagaac ctccagccaa aactactgca tgaacttttt
     5641 gaaccatatt tacagggtct aataaatcgg tttctctagt ccctggagca gagcccctta
     5701 catcaacacc gcaagtagct ccttcttcac atattactac tgtgcaccca gtaagaccat
     5761 ttctatcctc aatctgccca acctttaagc cttctatatc caaaatatta ttatacatat
     5821 cctttttctc ctcttataga aacttcgcct gaaataattt cttttatact tccatcactt
     5881 ctttgaacta ataaatttcc ctcttcattt atgtctaaac atttcactag ttctctatta
     5941 tctcctttta atacatatat atttttatct ataattgcag aataatctct acatattttt
     6001 aaggtttttt ctttggaatt atttacagta taatctaaat ataatttttc aaattcagtc
     6061 aatacattcc taacaatatc tactctggat aacatatatc cttctttata caatgatgtg
     6121 gctttgtcag ctatttcttg ttcaaaatcc attattttaa catttattcc aattccaaga
     6181 acaacataat ttgttctttc aatttcagca gataactctg ttaatatacc acatattttt
     6241 tttccattta aaataacatc atttggccat tttattaatg tatttgctcc tagattgttt
     6301 aaagctttcg ttacacttgc tcctgctatt aaagttataa atggagcttt gaatggttcc
     6361 atatctggtt ttaatataat actcatccag attccttcat gaggttttga attccatctt
     6421 cttcccaatc gtcctttccc catatcttga tgttcactta aaatcacagt cccatctaca
     6481 ctatttatag ctatcctttt agcatgttca tttgttgaat ctatagtgtc aagatgttca
     6541 atattttttc caataaaatc tgtatctaat ttatagtaaa tattctgtgg acttaataaa
     6601 tcagttggat tttccgctaa tcgataccct tttttattta cagattctat attatatccc
     6661 tcttctttta aggctttcat atgtttccat atagctgcac gtgacacccc taattgtttt
     6721 gataattctt ctcctgaaat aaattcagat ttattattta atataatctc tattatttta
     6781 tctcgcatat ttcacctcta atatgacata atttcatgac ttattttctt ctaataaata
     6841 attatacatt attaatatac ttttaatact attaatttta aatttccttt ttaattatta
     6901 taatggttgt tttatatttt atattgatga tttatataat tttacatttt atttaaataa
     6961 ttttaaaata ataaaaaaac tgaaagtatc aattaaatta atacatgcaa atataatttg
     7021 cttattaatt taatattata ctttcagttt tagtatttaa tccacatcag ataataatcc
     7081 taaaatgacc aaggtcttaa aactatttat ctttatcaga tttatcttct aaatttttat
     7141 ttatatcaat aacattgttt ctttcttctt catctttttc taattttata gttaattgtt
     7201 cttcaacttc tttagaagaa ttttcctctg taactgcatt ttcaatttca ttcagaggta
     7261 actctttatt aaatgctttt acaaattgtt ctgcatctaa agtttcatat ataagtaaag
     7321 cttgtgctac atactctaat ctatccatat tttcttgtaa tatacttctt gtcttatcat
     7381 atgcaccatc aactatacgc tttgtttctt ggtctatctc aaaagcaact tcttcagaat
     7441 aatttctctt acttgaaaaa ctattaccta ggaatacttc atcatcactg tcaaatgaca
     7501 ttggaccaag tttagaactc attccatact ttgttaccat acttcttgca gtagctgtaa
     7561 ctctttctaa gtcatttgaa gcacctgtag atacatcctt aagtactagc tcttctgcta
     7621 ctctaccacc aagtaaaact acaatatttt ctttcatttc attttttgta gcatagaact
     7681 tatcttcaac aggaagttgc attgtaaacc ctccagctct tcctcttgga actattgtaa
     7741 cttgatgaac aggacttact tcttcaagta catgggcaca aactgcatgc cctccttcat
     7801 ggtacgctgt aagtcttctt tctttctcac ttattacttt cgatttttta gccacacctg
     7861 ctataacctt tgttatagct tcttctatag tttccatctt aattttcttt tctctttttc
     7921 tagctgttaa tattgcagcc tcattcatta agttctctat atcagcagga gtaaatcctg
     7981 gtgttcttct agccaagaca tccattttta catcatcact taatggttta tttcttgagt
     8041 gaactttaaa tatagcctct ctacctttta catctggtgt accaacaaca acttgtctgt
     8101 caaatcttcc tggtctaagt aaagctgggt caagtatatc tggtctattt gtagctgcca
     8161 tgattattat accttgattt accccaaatc catccatttc aactagaagt tgattaagag
     8221 tttgctctct ttcatcatga cctccaccaa gacctgcacc tctttttcta ccaactgcat
     8281 caatttcatc tatgaatatg atagctggag cacttttctt agcctgttca aataaatctc
     8341 ttactcttga agcaccaacc ccaacaaaca tctcaacgaa atcagaacca cttatactga
     8401 aaaagggaac tcctgcttct cctgcaactg ctcttgatag atatgtttta ccagttccag
     8461 gaggtcctac cataagcatt ccttttggta ttcttgcacc caattcaata tatttcttag
     8521 gattctttaa gaaatctact acctcctgca aatcttcttt ttcctcatct agtccagcta
     8581 catctttaaa tgtaactcta gttttttcat catctttatg aaccttagct tttgatttgc
     8641 caaaactcat aacctttccg cctccacctt gagactgatt cataaacaca aaccaaataa
     8701 tcaccatgaa aaatattaac agtaatgtag gcaacatttc gacaaaccaa ggtgtagatg
     8761 gcttagcttc accaccaaaa gttagtttcc cagccttagc ttggtctaac acctcatctg
     8821 caagtttatt tcccatcact tctgttggaa catatgactt aaactttgta tttgtatctt
     8881 ttatagtacc ttctacagaa gtttgattta caaagtataa tcttgatata ttctcatctg
     8941 ttaattctct atatacttca gagaatttta aatcttttat tttttctgta ggcttacctg
     9001 agaattgaac tatacctact ataattataa aaacaagtag ataaaaacct gcccctttta
     9061 gcaacttatt caatatagaa gccctccttt catacattgc tttgccctat aatttgttaa
     9121 aattaatttt taatacttct tttgtgtttt catcaatttt ataattttca ctgattttat
     9181 aatctccaac acatattatt ccttcactat ctgtgattat aggaatttta catctttctt
     9241 ctcttgggat ttttaaatct atgaataaat ctttcacttt tttacttccc atagcaagct
     9301 ttatcttatc accttgcctt ctgcttctta ttactatacc cccttttacc ttattaaaat
     9361 caaacccttt agatgaattg tctaatttca tactcttata cctatctatg ctcattactt
     9421 gggtctcaat aatttgtttt aattctttta ttttaataaa cccattacta ggtacattgt
     9481 aataaaattc aatttcctca ttaacaattt cctcattcgt taatattata ctgtcttttt
     9541 ttctataaac aaatatattt cttggaagag ttaacatttt atttactttt gaattatctt
     9601 ctaaagacat tatatcctct atatgtcttt ggtcaacaaa attagtatct cctagtatat
     9661 gttttatgga atttctaaga actctaactt tgatagcatc atgtaagcaa acaaaatcgt
     9721 ctaaatttat ctctacaaag cctttttctt ttatatttga aacttctcta aatttagctt
     9781 ctgcctcttt ctcaatataa tcgttatcac tttttaaact attgctcatc ctcactatag
     9841 attctattac atttgaatta aaattatcct tcatatatgg taaaaggtct aaccttatct
     9901 tatttctagt gtatatattt tctaaatttg ttttatctat cctcggattt aaattataag
     9961 cttcacaata ctcctctatc tcatttcttt ccacgtctaa tatgggtcta attatgcaat
    10021 tatctctaat ataatcaatt ccctttaagc cttttaaacc tgttcctctc atgatacgca
    10081 tcataaccgt ttcggcttgg tcattgagat tatgacctat tgctatttta ttggctttaa
    10141 tcttatcctt aatttcataa aacatttcat atcttagctt ccttgctcct tcttctaatg
    10201 ataatccctc attttcacaa tattttggta cattaataga ctttacaaag aatataattc
    10261 ccatatcctc acaaagttta gatacatata atgcatcttt ttgagcttct atccctctta
    10321 tttgatggtt taaatgtgct gcatatattt ctatattaaa atcttttttt aatctattta
    10381 gtacatgcaa taaacaaact gaatcaggtc ctccagaaag acctaaaact attttatcgc
    10441 ctttttgtat taaattatgt ttatttatag tacttagtac cttatcaaat atcatattta
    10501 tccctcttac acttagtttt attcactttt aagtatactt tctataaaaa aacgctaaac
    10561 ccttgtatca attttaagaa attaaagttt aatataaata taataatacc aaataaatta
    10621 aataaaaggt agttttttca ctacctttat aaaatttctg gaaaatagcc atagttatta
    10681 tttagtacct atgtatatat ctattttatt cctacctaaa aataaatctt gtacctttat
    10741 attatagtct attttcaatt ttatgtgttc cccttcttgt atatttatat ctaattctct
    10801 tgtatcaata tcaattaata aagtaccttg tggggtcaca tactttgtag tactactttc
    10861 tccacattta aacagcattg ttgagttaat agcaccaaac tttttgatac taacttgatt
    10921 tttaagtatc tttatagtat tagttatttc tatattttct tctttttcct tgtaaataac
    10981 atatattcca tcatttttat aaaaaatttt accaaaggca gtcatctcta tggtgtccat
    11041 atttcctttt tcatcatatt gtctagtatt tatacttatt tttacactca acgatttttc
    11101 ctccatagat tcttcatata aactagtatt tttgtatttc tactttttga atcttatcaa
    11161 tttttttctt taagaactct cttgctatat ctgaaaattt ttcaggtata tctgatacat
    11221 aatattgata agttccatga atttctgtat tgtttataat attttgcacc tctaaaatct
    11281 tttttaaatc ctttgcagtc tctttagctg gattaacaag ttttatatgc tctccaactt
    11341 cttcacctat tgttctcttt aaaataggat aatgagtaca tcctaacact aaagaatcta
    11401 tatttttttc tttcaattct tgtaaatata tctttgctgt caatttagcc acttctgtat
    11461 ttgcccatcc ttcttcaaca ataggtacaa ataaaggaca tgctttatta acaatttcta
    11521 tactctcatc tattttagat atttccaagt tgtatgcttt tgaacttata gttccttcag
    11581 ttcctattat tcctacaatt ttgttttttg tagaacttac agctgttcta gctcctgctt
    11641 ctataactcc aattatcggt atatcatatt tttcttgagc ttcttttaag cttcttgcag
    11701 tagcagtgtt acatgctata actatagctt ttacaccttt tgtttttaaa aagtttatag
    11761 cttgaaaagt atatttaatt atagtttctt tggatcttga accatatggt attctggcag
    11821 tatctccaaa gtatattata tcctcatttg gtaatatctt cattatctct tttagaactg
    11881 ttaatccacc taatcccgaa tcaaaaactc ctattggttt attgttcatc tttatacaac
    11941 acctacttta catttttgtg tttaacaaca acttatttat cgctaaaatt tatatttata
    12001 ttcataatta atatattatt acctaaatga ttattatagt ttattttaca caaaaaataa
    12061 aggagctatc ttaaattaga aaataatttc tacaaaaaga caactccttt cactacttaa
    12121 aaatttaaat ttattttgca aacacatgat ttcctattct catataagga ttcaatctat
    12181 ttatccattt gcaagtggct atttctggat tccaaaaata tactgcttta ttagttgggt
    12241 cagttccata aagagcttcc tgagctgctt tacgtgctga gtctgttggt gccatcttta
    12301 ttgaaccatc aagcacaact gagaaagcat ttttttgata tatgacttct tctattgtgt
    12361 ctggaaatct agggtccaat actctattta ttactacagc tgctacagca acctgtccct
    12421 catagctttc tcctctagct tctcctgcaa ccaacttaga taataacatc aaatcattat
    12481 cagataaatt tattacttct ttctttgcta tatttttttc ttgtttgttt actttcttaa
    12541 tattttctgt tggttcatat gcaaatacct ctttaatagg cataaatata aaagtcaaag
    12601 ctaatagtgt agccatcagt ttactccacc tgtccatgat taaaaccact ccttacattc
    12661 ttatttttaa aattttttct ctaaatcctt gactacttca agtactttaa acttaatttt
    12721 tactctactt gttttttttg gtgtatcttg ttttattaac ttatattctt tatcagttaa
    12781 aacttccttc aacttatcat ctgtagattt atctatcact ccattagatt catcaacaaa
    12841 acatagtgga gggaacataa cacaccacca gttttttcct tcacctttcc caatgataat
    12901 ctttaatgct ttatattcac cagctggaag aactatatta gaatattgtt tagtagggaa
    12961 attgctatat tgtataccta cctttacact gtaattatat ccatttttaa gaataatatt
    13021 tttacttatt tcttccagac tactatattc cttttttata atagcttcac tttcttttat
    13081 actttttgaa ttttgaagct ttggctgtaa atattttatt acttcatctc taacttttag
    13141 tttaagttct tggtcttcat ctgtattgct gtttgctata acatgaaatc ttattaattt
    13201 atctttataa tcttttgata tattgtctac tttttttacc tcaccattta ttactattgt
    13261 cattattgat atgactgata taagacttaa tattaatagt cccaatctaa tcttaatttt
    13321 tctcattgaa aattccccct caagataaat ttatatcatc aattatgtcc aattaaattt
    13381 aatttattca taatattttt taatactaat aaaaaaagct atcttaaaat gattacaatc
    13441 attttaaaat agctttttaa accttatatt ttataagtct ttactaatca tagccttatc
    13501 tatcataaac tgctctgctt tttctatatg ctctgtagca tacttcttgg atagctcact
    13561 atcaccttta attatagcat caagtaaata tttatgctct tcaacaatat tatgtgatgt
    13621 atcataagca gacatatagg taactctaaa tctatatact tgttcccaca atgagtttat
    13681 taattggatt aattttttat tattagttgc tttaaatata cattcatgaa attctacatc
    13741 aagttttaat aacgcatcta catcggattc caaagtgctt tttttaaatt cttctgatat
    13801 atctttaagc tttttaatat cttcatcact aattctttca gcggcaagat atgctgctaa
    13861 gccttctagg cttgctctta cttctaatac atccataata tcctttaatg acatatttgc
    13921 cacatacgca ccttttctag gcaacataac gactaaacct tctaattcaa gctttcttat
    13981 tgcctctctc actggagtcc tacttactcc taattgttct gctagttgca cttccattag
    14041 cctttgacca ggctttaatt ttccttctaa gattgcttct cttaaattct caaagactac
    14101 atctcttaat ggcttgtaat tatccaagtt cagtttagtt aaattctcca caaatttcaa
    14161 cccctttttg actgctatta acaacataca cctgtttata tttttttaat aattcttttt
    14221 ttccaattag agcatcttct ttatttttaa atagtccaaa aactgtagga ccacttccac
    14281 tcatcataga acctaaagca ttatttttca tcattacttg ttttatatca cttatttctt
    14341 tgtgtttacc tatagtaaca ttttcaagaa tatttaccat gctttctgat acagctttta
    14401 tatcttcaga ttttaaacac tctatcaaat atttattatt tggtcttttt ttaatattat
    14461 tcaaatctaa tccttgataa acttctttag tagaaacgaa taagtctggt ttgcatatca
    14521 atatatttgt atcacatggt aaacctttga tatttgttag cttttctcca ataccttgag
    14581 ctaaggcagg tcttcctgat atacaaaaag gaacatctgc gcccaaatta agtcctattt
    14641 cttttaattc atcttcagat aatctaagtt cccataggtg atttaaacct actaatactg
    14701 cggctgcatt agaacttcct ccagccatcc ctgctgctac aggaatattt ttttctatga
    14761 atatttctac accttttttt atataaaatt tatttttaag tattttggca gctttataaa
    14821 caatattatc ttcatctaaa ggaatatcta aactagtgct ttttaccttt atttcatctt
    14881 cgtctaattc ttttatcttt actatatcgt ataaatctat cgtttgcatt atcatttcaa
    14941 caaaatgata accgtcttgc ctttttccca agacatcaat ggataaattt atttttgctc
    15001 tactctttag tcttataaaa tccattctat cctccttgtt taaagaataa tgcatcacta
    15061 acaataatat tatactacaa aaaataaaat aatagggtat tataataccc tattatactt
    15121 aattacttta aattttttaa tattaatcta ctaatacgac ttttttttct aaaataagac
    15181 acttaccagg cttaattggt tcatcaagtt ttatatcatt aagttctgca atttcatttt
    15241 ctgttgtatt gtattttttt gctatattcc agaaagtatc tccttctttg catatatata
    15301 cgattatgct aggagcttta gataaatcat atacaccttg gtcttctccc ttgataataa
    15361 aattttctgc tttcttatct aatgcttcag taaatctctt aattttgata atcaagtcta
    15421 tctggtccct attcaagtca acttcgactt tatcaataca tgctgtatta aacacagatg
    15481 ctgtatcagt tagattatcc atttctatat catgttcaaa tggaatttcc tcactaattt
    15541 tataaaccaa ttttaaacct tcaactggga caaataaaat ttcaactttt atgattcctt
    15601 gtataatgct tttatttcct tcaatataac tattttctat agacatggtt ggacaaacgc
    15661 ttacaatatc ttttatttgt atatcatcat tatcatttct tatgccttct ctaaccataa
    15721 atgtttcttc actacgtctt aaagtcttat ttaattgaat aggtttgtgg tcaaatttta
    15781 ttatcttttg aggcgaatat gcatcttgta atacttctct tgtaacttca tctgtaactt
    15841 ttaccttaca acaagccata caatctattt ctaaaagacc tgtgttgctt tcactatttt
    15901 gcttgaaaat atgattaaaa tctgacatag acaacaatac ttcttcagtc ataccatcac
    15961 taactccagg gacttctaca aattgagtaa actcaatacc tactctatcc aactctacaa
    16021 gttcaccctc atatgtacaa gctaacggat ttatttctaa tactccacca ataataactt
    16081 tattgtcagt cactctactt tcttttactt taacacatgg atttaaactt attatagatt
    16141 gaatttcctc tgtgtttatt gttatagtat ctcttataga actttctgct ttttctattc
    16201 caactatatc ttgaaaacaa atttcctttc tgtgcttttg tatgccttcc acttgcgcca
    16261 catcttttac aatgtctaac ctttgctttt caaacaaact tccccttata ttcataagag
    16321 ctcctacttt aatctttctc tcattcatta tagtacaatc catatgctct acttctgaaa
    16381 ataacatgta ttccatatct tgaataacat tgtctttttc tattacttcg tttatatcta
    16441 cttttccgtc tacattagaa atagtatttt tatcatctgc aatatagatt acattataat
    16501 taaaactacc tctgcaaagt atcttcccat ctgccatttc aattttttta agtgatatgt
    16561 atccttctgt ttttacaatt tcatatacat ctaatttttt gtctggtaca actgcctccg
    16621 cttcaatgaa tgtttgaaat tttccaaagt ctattctatt gtcaacttta attacatctt
    16681 taattaattc catagtctac ctccttaagt attataaaaa tatcacacta tatttatatt
    16741 taaggagcaa aaaaaaaatt acacctattt ttaaataggt ataattttaa cttatttgta
    16801 atttatcttg atctctaaaa acttgcaact ttacattaga tgttaataaa tctgagtaac
    16861 tataagatac tctaggatat ccattacttt catcatctaa ctttataaca aacacgcttg
    16921 gataaacttt ttctagtata ccctcttttg taataatctg ctttcttcct ttattagctt
    16981 tcaacaatat tttctttcct atatgtctct ctaagcttac tcttatctta tctagagttt
    17041 gaacagtagc cacaatatca ccttctttaa cgtttatatg tttacataat atcacatata
    17101 aacatttttg tcaaatgttt aaactaatta ttttatatat tttttgcaaa aatgtcaata
    17161 ctatttttga agatatacag tataattatg ttaatttttg ctaatgtaat attgtttttg
    17221 gcattgccca tctaaatttt cctcttgttt cagaaccacc aattccctta acacctgtaa
    17281 tagtctgctc tataacctca tcaataggaa ctactacatc aattcttgaa caagcaattt
    17341 tctccactat aaaaacaggg tctaatgcat gtatcattat tcttccaggc gaacttgcat
    17401 agtttgctcc tgcacttatt attttttcat aatttgattg gcatgctcct gcgaatatga
    17461 caagattatc caaatttggt tgccatttac gtgcttcttt tactgtttta acaaaatttg
    17521 aagagtttct atagttactc atatcttcta tatctcctct ttttattgtc attgcatcat
    17581 gacctgttat tactagtata tccggattat atttttctag tagtgctctt acttctttat
    17641 actgattttg ctctgaaaca gctacaccta ctgcaggtat tccaagttga gcatatacat
    17701 ccaagcaaat ttttaaatat tctttatctc catctatctg tagtactttt ccaggcattc
    17761 catatgtgtt aggatttaat tgaagttttg gaactgctct agtcattctt ttttgtcttt
    17821 ctttagcttt tcttactgac ttatataata aattttctac atttttatct attaatatat
    17881 cttttatatc aggtgctttt actaattcta ggtcatcaat ataagcatct gctattactc
    17941 taaatgcaat tccctttagt atagctattt tctcattatt ttcatctaca ccaaaagaga
    18001 ctattttaaa tactatatct ttgttatgtg actttctagc tacaatgtct cctaccttca
    18061 tagttaaacc tccaataaat ttttatctta gtttatacta tgttcgatat aaaactttgt
    18121 ttcctaatgt caaaaaaaca tttcttataa tacttctttt atcactctta aagcattttt
    18181 atagtatatc ttttctatat catcttcact aaacccttct ttttttagag gttcatacag
    18241 tttacccatc tgagatatat cttcaatctc aacttttgaa tctattccat caaaatctga
    18301 ccctagagat acaacatcta ttcctccaac atttacaata tgttttatgt gtctaaccat
    18361 atcttcaagt ttactttcac ttttatcact taaaaataag tggaagaagt ttatacctgt
    18421 aactccacct ttatttgcta atactttaat catatcatca gttaaatttc tagaatgatt
    18481 catcattgct cttgaattag aatgtgtagc aattattggt ttagaagaaa tctttgcaat
    18541 ttcataaaat ccaccatctg aaatatgaga aacatcaacc aacattccca attcattcat
    18601 cttatgtacc acttctcttc caaaatcagt aagccctttt tctctatatt ctactttatt
    18661 atgaggaaag ctcaattcat tcacatgatt ccaacttatt gtcatcattc taacccctaa
    18721 gtcgtaaaat ttctttaaat tttctaattt accttcaagt actgcacctt cttcaataga
    18781 taaaagagct gtaagtttac cttctgattg gtttttcata atttcttcat agttagtagc
    18841 taaagctatt tcatcactat tttttttcat ttcctcatga aatttatttg ccatactcat
    18901 gcaataatca aaaggatgtt ttacttcttc agtatcaaca aaaagagcaa atgtttgagc
    18961 taatgaatcc ccctttttca atctgtctat atcaacagaa tatttattag acttaagttc
    19021 acttgtttct acattttcca ttaattttgc tatagtatca caatgtaaat caataaattt
    19081 catatttata ccccctgtat attttatatt tatatctaaa aatagaaata gttgtcctta
    19141 tagacaacta ttgacaacta ttttttaata ttctttttaa tattacttaa tcttattctt
    19201 cttttaagtt aggtattact tttttaactt cttcaatttg atgccatgtt ttatctatat
    19261 tattttcact aatatatttt tttaaatcag ttaaagatgt aaaatcttta actgataaat
    19321 tgtcatcatc atctgcaaat cttaaaataa acatgagact cccttacttt ccacaacatt
    19381 tcttatattt ttttccgctt ccacatgggc atgaatcatt tcttcctatt tttgcttctt
    19441 taactattgt tttagacttc ttataagtct ttattatttc ttttctttgg tctgctggta
    19501 gtatatcttc ccaaccttgt aaattatata gccaatgagc ctcaacagtt accatattgt
    19561 tatataattt ttcccaatct atttttatag aaacttcttt atcagcttct acttcttcta
    19621 aaactatatc ttcttttaag ctttcactta ttccatctat gaatcccatg aaaaattcat
    19681 tagtagttcc aaatttttca gccaattcac ttactttacc ttcaacaaca tccattttat
    19741 tatttaatat ttcattgtat attccagctt caactttcaa atattcttcc cagaatttta
    19801 cttctgcttc ttgactttca tgattatcac ttaaatttct ccattcagta tataaactca
    19861 tactttttcc tcctaagtat acctatattt ttatagtttt tatagtataa aatcttttag
    19921 ttagattgga agtttcttct ttttgattat accatattct atttaaagtt aactcttata
    19981 ttatatcata attaaacaat tcttttaata cttttatagc atactcttgg tttataaaat
    20041 aagggcttgt cgcaattgtt attactttgg cttttttaat taagtttttt atcttgtaaa
    20101 atttgagttc aaatggtata tagtccatat cacttgaaaa tatatccaaa tctatgtcaa
    20161 gaacaaactc tgattctact tctaaatcaa atccatatag actatctatt attattaatt
    20221 ctgaaaatat gttatatttt aaagcaggtt ttataaaact tcccacattt aaaacttcat
    20281 ttgtatatct aaatacatct tttagattat ttacatctac atcatagttc tcaggttctc
    20341 tagtatcctt atgttggtct acatgcacta acttgcatcc ttttgtaaac tcacccttta
    20401 acaaagattt tgcccaaaaa taaaatgcat ggttatgatt gtcaaacaca tatatctttt
    20461 tatttcccag gacatattcg accatattct ccaatccttt tgcttttatt tccttatttt
    20521 catctacttc attaaatact acctcattgc ctaaccttac atcatttaat gtaccattta
    20581 ttagttttgg aacgtatatg ctcttatttt ctcttttatc atatgaaaat acattatttc
    20641 ctactggttt ttctatataa aatccactat attcataatt ttccataaaa ctttatttcc
    20701 tccattgtta tataaatata taagttataa ttttaaaaat taaaatgcac ttattgtaaa
    20761 tcatatcatc tattatagaa aacatcttat ataactaaat ataatatctt ctctcaatat
    20821 gtattataca ataagtgcaa aatatattta tataaaatat actacattcc tataacaaaa
    20881 ccatcagctt caattggaag gtaactaact cttctattca actccacctt tccaaaaaat
    20941 actacccaat atggtctaaa aatactttca attttaacta tttcaatctc tgggattcct
    21001 cccataaacc tatgagttaa tctcatagcc attttatttg cctttgcttt tactcttcgt
    21061 tcgtccttat cactatactg cacaatatta caatccacat tttttacaac ttcatatgta
    21121 ggaaaatctt gtactatact accagaacct gtagaaccat tcactaaaat ttttaattca
    21181 tttatatcat tagaatatct cctattcttg aaaatggatt tcgttttgtg aattttaagt
    21241 gtgataattt taaactctat ataatgaagt ctaacttcat gagcatattt gttctttaat
    21301 attagcttac taaataaaga attttttcca cttttagcta tctctatagc ttcttctttt
    21361 gtaattctta taggtaacgc tcttatatcc ataaaatcat tctctttctt atttttttct
    21421 tgtcattttt aaaactctat atatataatc aaaaaatatt acaattacaa ctgccataac
    21481 tccatatgga attatttttc caactataat atcatttcta gtcaatatac cataaaatat
    21541 aaatcctgtt attaaaaagt acaataatac tagaaaaccg tctattccaa gccattttat
    21601 ttctaaatct ttttttacct tagacactaa tttagacata gcagtttctc cttttataga
    21661 ttattttgtg tatttaaatt ttcacctatt ctattaccaa tcatccatgc aataaatgaa
    21721 aaaggtatag ctatcacaag aggctctaac tcaaagctct gaactggaaa tattttaaaa
    21781 taaccaaaag tcactccacc aacaatcatt cctaaaatac cacccataga tgttcttctt
    21841 ccatttccaa gaagcccacc tactataggt acaaatgccg aacccatcca aatacttcct
    21901 acaaatatac atgcatcata tacattttca aatctaaatg ctacgctaag ccccataact
    21961 gctgctaaac atacacctat tctagtaaac tttactaatt gtttctgaga taactctttt
    22021 tttgtaattc ttccatatat atcatttgct aatgtagccc cagctgctag ataatagcta
    22081 tccaatgttg atattataga acctaaaatt cctattataa atattactct tattccttgt
    22141 ggtaatgtat ctaaaactaa agttatatat ccagcagctg gagccatttc tggataagta
    22201 gctcttacta taagtcctgt catagttaac accatgtcaa aacatatcca tagtgaaaaa
    22261 caagttaata aggctcttct tccaacctta ggagaatctg ctgctgaaaa tctttgataa
    22321 aaagttgggt ctgaataagc tccaaaagaa tatacaatta acatcaatgc tttcatagct
    22381 gacatacctt ttgtagggtg cataagctgt ggtgcatctt tgagtgcttc tgtaagacct
    22441 tgaaaccctc ctatttgttc aaattgacct ggcataacca tagttaaacc aaataccata
    22501 caaaaaaaca ataccatatc tgtaatggca actcccataa gcccaccaag tactgtaagt
    22561 acaacgacta ctcctaccat tattaatcct attaattctt tatctatttt ccatgctacg
    22621 ttacctatag ttcccatagc agttatttct ccaagttgtg cacaatatat aaacatcaaa
    22681 attgccccaa ttatagcaac atgcttgcca taaaaacttt ccagtaaatc aggaactgtt
    22741 atattggttc ttatatgaat ccttggacca acccacaatg ctaaaggcat tctcccaata
    22801 tgtgcagcta ttgaccaaac tgcccatgca gatattccat atatagccgc atactctaca
    22861 ctacctactg taccaactcc tccataccat gatgcaacca aagttcctac tattaaggac
    22921 catggtaaac ttcgatttcc taggtaataa ctttccatat cttttacatt tctagaaaaa
    22981 taataaccta ttcctaataa tataaatgca aatataacta taatcccatt ttcccaaaat
    23041 gttaacatat ctaatctcca cattcttata ttttaatttt taactttaat atctaaatat
    23101 gaacaactct acccatttcc ataaaaatag gtagagttat ataataatta taagtattta
    23161 agtaatcttg ttattaagca attttattgt ttaaaccttt attttccctt ttatttattt
    23221 tatttaaagc ataagtagca aatatcccta taactgcaca tactacagat aaaataaata
    23281 tatatgtata tcctgtttgt ccatatttat ctaaccaatt acctgcaata gtgtaataaa
    23341 acgcatctgg agaaaatcct ataaatgaag caaatccaac tactgctcct gtagtttcta
    23401 aagatatatt agactctgaa acagatgcaa agtatatacc tctaaatcca aataaaatta
    23461 cacttagtat tatcatgttt attacagcta tccatataaa tcctgtactc ttaggaataa
    23521 ccaagtaagc agctgtactt actgtcatta atataaatcc tacaaacatt actcttatag
    23581 atgaaccaac tttatctgtt ataacaccag caactggaga tgctcccatt tttataacat
    23641 aagttcttat tattgataaa gcagaaacta gagtcattgg cattacataa acttctgata
    23701 agtatggact taaataagtt aaacttgaga atactaaata agttgaaaat attataagac
    23761 caattaacca agcctttggc attgtaacta cttttaacaa tgacttaata cttaatcctt
    23821 catctgatgc tccactatct tttttctctt ctactaagaa aaatagaagt ataccacata
    23881 ttatagatgc tgcagaatat aaccatacta catatttgaa tcctactgtt atttcagcaa
    23941 atttaccaaa gaaataaaga cctataaaag aaattaatgt acctgatacg cctgataacc
    24001 cttcataaaa accaaatatt cttccttgct ctgactcact tccttgcatt ctaacaacct
    24061 taacagaggc tgagaagaag gttaatattg ttgtaaatcc ccataataca aatattaaaa
    24121 gtatcatact atatgatggg aatgtagaga aataaaatcc aagtattcct gaagcaatta
    24181 aagaaaaagt tattaatttt ctaaccgaga atctatctgc aaaccatcct cctataaaat
    24241 aagataccat tcctactaat ccatataaac tcaataagtt ccctatttgt gtatggctca
    24301 atgataaagc ttgttgcatt gggtcataaa atgatgattt catatatggt aaactataca
    24361 tagccgttgt tccaaatgcc aatatgaata ttatcaagaa tcttttaaaa ttacttttgt
    24421 tctccattga ttttctctcc ttaaaatttt attagttata actttattca tataatttta
    24481 ttttaatttt tctattgctt gtttaaaatc atttattata tcatttgaag attctaatcc
    24541 tacagctatt cttattagat tttccccatt catttgaact aatgtatgct cttcccctaa
    24601 gcttgttgct attgttgcaa gctctaaaga attcattaat cttctactag cttggtctct
    24661 tgtcaatcca ttgataccat ccttaagttt aaatgaaact acacctccac ctaaaccatt
    24721 catttgagtt ttacaaagct catattgagg atgtgatttt aatgctggat actttaccaa
    24781 ttccacattc tcttcttcct ctaaaaactc tgctactgct aatccatttt cacaatgctt
    24841 tttcatcctc atatctaacg ttttcatacc ccttaaaatc atccatgcac tcattggtgg
    24901 taaagatgct cctgtaaaac aacataaact tggccatcta actaaatcta ttaattcttt
    24961 tgaacctgct actgctcccc ctaaagtatc tccatgtcca tttataaact tagtaagact
    25021 atgtattact aaatctgcac ctaaatctat tggtctttgt acaattggtg tcccaaaagt
    25081 actatcaact atagctaagc ttttattttc atgagcaatt tctgctattg tttttatatc
    25141 tactaagtct aatgatggat ttaaaggact ttctatataa actattttag tatttggtct
    25201 cattgcttct tttacatttt gtggatttac tgcatctaca aatgtaactt caactccaaa
    25261 tttagataac agctcagaca tgaataattt agtgtgtgaa aatattccat ctgaactaac
    25321 tatatggtca ccacttttta ccaatgctaa tatagtagat gttattgccc ccatccctga
    25381 tgtactagat atggcacttt ctgcattttc aagcatagcc aacttagctt ctaactctgt
    25441 aagagtagga ttacactctc ttgtatacac atatccccat gtcatacaca actcttcaaa
    25501 atgctctact gtctttgcag caaatgttgc tgtttgacat attggtgtag ccaatgcacc
    25561 tgttgttggg tctgcactat gtccccaatg tattattttt gtctctaaat cttgattaat
    25621 attgttactc attttgattt cctcccattt tgacttattt atacaacgtt ttccctacca
    25681 aatttcatca atttagctta gtcttaatac attttttcaa gtatactttt ttatttgtta
    25741 taatttctaa aaatatgaca cttattttgt ttttttacag ttctaattat aaactctata
    25801 gtaactatag agtcaatatg tttttgatat tttttttaca atgtttaaaa tagtcagtac
    25861 aaaaataggt atatcttaga ataactaaag tcatcctaag atatacctat ttttatatca
    25921 caatataaat aattacatat aagcattagt ttttttatca taaatagatt tttcaagatt
    25981 tatcatcttt tcataaatat attatagtta aatattttaa tattacctaa catctacaat
    26041 aaaatatact cttaaaataa aattatataa ttaaaaatac tctataaaag ctaaaaagtt
    26101 tttctattcg ctacatctaa aaataattag atagaggtat aaatgtattt agtttattta
    26161 tacctctagt cttttataaa ttatatctct actaaattga ttttttagca cgaataagtg
    26221 tgctcttgga gataccagtc atcttcacaa cctctctata tggcttagaa tttaataacc
    26281 ccaatgcata ttgtatctgt tgattgctgt atttctttgg cctaccatct ttaaagtcct
    26341 ttttcttttt agcaaattct tttcctaatt gagtacgttc tattatcata tttctctcaa
    26401 gttctgcaac tgcaagtaat gtagtcaaaa agaatttacc cattgaagta ttttctaaaa
    26461 ggcctatatt taaaatatga acacttactt ctttagcaaa taaaccttct attagttgta
    26521 ttccttctga tgtacttcta gctagtctat ccagcttagt tgcaacaagt aagtctccct
    26581 tttttagaga tgatataatt ttttcaaatt taggcctgtt tgtagtagtg cctgtataag
    26641 tttcctttat tattttagca tcattatatt tattcaaaat ttcttgctgt tgctgataca
    26701 aactgttgtt gtctgtttga ttttttgtac ttacacggca atatccatat ataataatca
    26761 tcacttccta acataaagat tttgtacaga agttacccag aagcttgttt ttacttatct
    26821 ttatccattt tactaaaacg atttttgggt
//
LOCUS       DAGSWI010000050        26422 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.050, whole genome shotgun sequence.
ACCESSION   DAGSWI010000050 DAGSWI010000000
VERSION     DAGSWI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26422
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.050"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..508
                     /locus_tag="MJF80_001898"
     CDS             <1..508
                     /locus_tag="MJF80_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0280561.1"
                     /translation="YIYFKVSEKEEIQDIIEKIIIELYEPSVVNKVKIKLLVGLLLVE
                     LVKNSEDVENHAVDNYEKMMIIEILKYIDKDYKKGSLSEIASNLNQGDYKISKLVKKH
                     LGYTFKDLVQEKRLSKACELLVSTNLSIAEIIENVGYENLTYFYKIFKNKYGITPKEY
                     KKNIKNSQ"
     gene            715..2109
                     /gene="rhaB"
                     /locus_tag="MJF80_001899"
     CDS             715..2109
                     /gene="rhaB"
                     /locus_tag="MJF80_001899"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBZ0280562.1"
                     /translation="MRYYLAIDIGASSGRHIISYINNGKLEIEEVYRFKNNLIKKDGH
                     FCWDISKLFSEIKNGIKKCIKINKIPVSVAIDTWAVDFVLLDEKDNILGNCVSYRDNR
                     TDGIMEEAFKIIDKKELYLKTGIQFQKFNTIYQLLAIKNQQPDLLEKAKSFLMIPDYF
                     NFLLTGKKVTEYTNATTTQLVNTTTNNWDEEIINKLGFDKMLFAKIKTPKTILGSLKK
                     ELVEELGCDLEVILPVTHDTGSAFMSTPVKNRSILLSSGTWSLLGVELERPKCKLEAL
                     DYNFTNEGGYEYRYRFLKNIMGLWMIQEARKCYDSKHSFTELVEHARLSEGFKSIVDV
                     NDDRFLNPENMVQEIIKYCIETNQTEPQTVGEVAYCIFNSLAKSYSKSINEIEEIIGE
                     KLETISVFGGGCQNELLNELIAKESGKKVLAGPVEATALGNIVCQLIAKNELNDLEQA
                     RDIIRNSFDIIQYN"
     gene            2130..3386
                     /gene="rhaA"
                     /locus_tag="MJF80_001900"
     CDS             2130..3386
                     /gene="rhaA"
                     /locus_tag="MJF80_001900"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBZ0280563.1"
                     /translation="MELKQRYEVAKKEYEKWGINVEEVLRVLSKVKISIHCWQGDDVT
                     GFEGNQQRLSGGIASTGNYPGKARNPQELRRDLDKALSLIPGKHKVNLHAIYAETGGS
                     FVDRDELKPEYFENWVKWAKERGLGLDFNPTIFSHPKSSDGLTLSHPDKKIRDFWIKH
                     CIASRKIGEYFGKELGQTCLTNIWIPDGYKDIPSDRLGPRRRLKESLEEILKVEINRE
                     YNLDCVESKVFGLGVESYTVGSNEFYLNFASKNNIISLLDTGHYHPTELVSDKLSSML
                     LFSDKVALHVSRPVRWDSDHVVILDDELKEIAKEIVRNDALDKVIIGLDFFDASINRI
                     AAWVIGTRNMIKALLSAMLMPHEELRKLQDEGDFTKRLAIMEEFKTYPMGDIWNYYCK
                     INNVAVKEDWIEVVKEYELTELLNRK"
     gene            3397..4212
                     /gene="rhaD"
                     /locus_tag="MJF80_001901"
     CDS             3397..4212
                     /gene="rhaD"
                     /locus_tag="MJF80_001901"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBZ0280564.1"
                     /translation="MSIMEMNFIKDFMKITYDAWLKGWHERNGGNITYRLTPEEISGI
                     KEFITEEIEYIPIGVTVENLANEYFLVTGSGKFMRNISINTVQNVGIVKIDEKGENYA
                     IVWGLDKAKPTSEFPSHLMSHSIKKDKTNGLHRVIMHSHTTNLIALTFILPLEGKVFT
                     RELWEMATECPVVFPEGIGIVPWMVPGGIEIAKATQNLMEKHNIVIWAHHGTFCSGET
                     LDLTFGLMDTVEKSAEILVKVISMGGKKQTITGDNFRDLSKNFKVSISEDYLY"
     gene            4489..5955
                     /locus_tag="MJF80_001902"
     CDS             4489..5955
                     /locus_tag="MJF80_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBZ0280565.1"
                     /translation="MKREVTIQKQAISIVLFMAVLFCGLLLPMINNKSEYSLPIESIM
                     FISASIISLFIVVTTNKTWEEVFESGLDGVKGALPGLIILLVIGPLIAGFIMSGLMPM
                     LVYYGVKLINPRFVYATALILSALFSSFTGTSWGSAATIGVVMMGVGNAIGANNAIIA
                     GAIVGGAYFGDKMSPLSDTTNIAAIASKVNLYDHVASMLWTTGPATIIALVIYIACGF
                     IFPATNTNLNSPEITRLLADLSGMFNFNIILFIPLLIVLVGSAMKKPAIPVMTIAAFL
                     SLAFAVIFQGFSLADVLIGLNSGFSLDMVTWHEYAKPIEGQAYILGFFQKGGFWELGK
                     LLPISTSILFVVGVLGSIDAMPATVNVIFGWVKSRTAIICSSLLTGIAMIAMTANGIA
                     CSFVTAGIFGDKYDENNIDRRVLSRTTEDTGTLLEVLFPWTPAAIFFTQTLGVNVGEY
                     ALWSIVNWGTPIIAVILAVTGIGTYKNSKDKKQSNKVS"
     gene            6055..7245
                     /locus_tag="MJF80_001903"
     CDS             6055..7245
                     /locus_tag="MJF80_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HBZ0280566.1"
                     /translation="MDKNKYKIETLMNHVGENLDGDYGAVNPPIYQSSIFARSTYEDI
                     EAYFEGSKDGYLYSRTKNPTNDILEAKLSALANAEASITFGSGMGAISSAILHFVKNG
                     DHIIASNNLYNPTMNFITKELKEKGNIENTIVEGDRIEDFENAIKQNTTLIYLECPST
                     AVFKMQDIKAIVALAKTHNIKTVIDNTWATPLYQRCIEMGIDLEVHSLSKYLNGHSDV
                     VGGCIIGSKKDIEEIRGSELALYGAKLAPFEAFLVIRGLRTLHARLSVHTKNAKEVVE
                     FLDNHSAVTKVYHPLSKYFNQKKLAKKQLLNYTALLSFEIDAKDVNDVKKFVNKLDLF
                     KIGVSWGGHESLVFAPNIAILNEMTLEQAKALNVSPYTIRISLGLENSEDLINDLNDA
                     LSIL"
     gene            7438..9396
                     /locus_tag="MJF80_001904"
     CDS             7438..9396
                     /locus_tag="MJF80_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HBZ0280567.1"
                     /translation="MKRAYYSSSVNEFIVKDIFTIFGEITCNDQFAADDLQKNTWKKE
                     IGILKRELSTFKDGHLLFEYTIPRIGSRIDNVLIYKGIVFLLEFKVDEDTYPKHAIEQ
                     VTDYALDLNCFHKESHDKLLVPILICTKAPQKDQRIRMMKENILETYCCNEFNIGKYI
                     KKICTTYNRSSFNSDTWINSLYMPTPTIIEAAQALYMGHNVEDISRNDASAKNLNQTT
                     KAINKIIDYSKTHNRKSICFITGVPGAGKTLAGLNIAIERQKVDENEHAVFLSGNGPL
                     VDVLQEALARDDVTRNGIRKSEAIRKAKEFIQIIHHFRDEAISVDTPPIERVTIFDEA
                     QRAWDEPNLTNFMKRKKGVLDFNMSEPEFLISILNRHIGWATIVCLIGGGQEINKGES
                     DGISGWFESLRNNYSDWDVYISNKIIDEEYSKGNSFDELTEGVNYRIIDDLHLSVSLR
                     SFRSENVSTFVKAVLDVDKVAAKELYNQFKKDYPICVTRDLELAKKWVRDKSKGSQRY
                     GMTASSGAKRLRKYGVWVQNKIDATNWFLNGKDDVRASYFLEETATEFDIQGLELDWT
                     IVCWDGNLRFKNNHFEYYNFNGTKWQNINKEDNILYLKNAYRVLLTRARQGFIIFVPI
                     GDESDITMQPNFYNGVYEYLKEIGIEEL"
     gene            9939..11312
                     /locus_tag="MJF80_001905"
     CDS             9939..11312
                     /locus_tag="MJF80_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBZ0280568.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            11567..12634
                     /locus_tag="MJF80_001906"
     CDS             11567..12634
                     /locus_tag="MJF80_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBZ0280569.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            12778..13086
                     /locus_tag="MJF80_001907"
     CDS             12778..13086
                     /locus_tag="MJF80_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBZ0280570.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            13079..14509
                     /locus_tag="MJF80_001908"
     CDS             13079..14509
                     /locus_tag="MJF80_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBZ0280571.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            14587..14961
                     /locus_tag="MJF80_001909"
     CDS             14587..14961
                     /locus_tag="MJF80_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBZ0280572.1"
                     /translation="MKKREDDFEVRRKHLQELSEDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            14955..17159
                     /locus_tag="MJF80_001910"
     CDS             14955..17159
                     /locus_tag="MJF80_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBZ0280573.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            17159..18568
                     /locus_tag="MJF80_001911"
     CDS             17159..18568
                     /locus_tag="MJF80_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBZ0280574.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            18553..19617
                     /locus_tag="MJF80_001912"
     CDS             18553..19617
                     /locus_tag="MJF80_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBZ0280575.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVAKKIIV"
     gene            19647..21068
                     /gene="nhaC"
                     /locus_tag="MJF80_001913"
     CDS             19647..21068
                     /gene="nhaC"
                     /locus_tag="MJF80_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBZ0280576.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VIFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            21497..21961
                     /locus_tag="MJF80_001914"
     CDS             21497..21961
                     /locus_tag="MJF80_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBZ0280577.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLVKLDVEVGFPVATYIDGKNEILQKIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            22195..22557
                     /locus_tag="MJF80_001915"
     CDS             22195..22557
                     /locus_tag="MJF80_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBZ0280578.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            23152..23988
                     /locus_tag="MJF80_001916"
     CDS             23152..23988
                     /locus_tag="MJF80_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0280579.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            24072..26186
                     /locus_tag="MJF80_001917"
     CDS             24072..26186
                     /locus_tag="MJF80_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBZ0280580.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLENVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMVEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNDQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTCELTQLIDM"
BASE COUNT        10518 a         2881 c         4890 g         8133 t
ORIGIN      
        1 atatatatat tttaaagtat ctgagaaaga agagattcaa gatataatag aaaaaattat
       61 aatagaattg tatgaaccaa gtgtggttaa taaggtaaag attaaattat tagtaggatt
      121 attattagta gaattagtta aaaattctga agatgtagaa aatcatgcag tagataatta
      181 tgaaaagatg atgattatag aaatactaaa atatattgat aaggactaca agaaaggtag
      241 tttatctgaa attgcaagta atttgaatca aggtgattat aaaataagta aactagtaaa
      301 aaaacatctt ggatatactt ttaaagattt agtgcaagaa aaaaggttaa gtaaagcatg
      361 tgaattatta gtatctacta atttatcaat agctgaaatt atagaaaatg taggttatga
      421 aaatttaact tatttttata aaatatttaa aaataagtat ggtattacac caaaagaata
      481 caaaaaaaat ataaaaaata gtcaatagta aaattagaat ctataaaata gattcttttt
      541 tattatcaaa taaaccattt taatagctct gaagatatta tattttgaaa attattattg
      601 agaataaagt tatattgcaa attaggttaa taaatatgac aaatttagat attaaatgaa
      661 tatactaaca tcttgtataa ttcaattaat aaataaatct aaggaggaaa agtcttgaga
      721 tactatttag caattgatat aggggcatct agtggaagac atattatctc ttatataaat
      781 aatggaaaat tggaaataga agaagtatat agatttaaga ataatttgat taaaaaagat
      841 ggtcattttt gttgggatat atcaaagcta ttttctgaaa taaaaaatgg gataaaaaaa
      901 tgtataaaaa taaataaaat tccagtaagt gtggctatag acacatgggc tgtagatttt
      961 gtgttacttg atgaaaaaga caatatatta ggtaactgtg tatcatacag agataataga
     1021 actgatggaa ttatggaaga agcctttaaa ataattgata aaaaggaact atatttaaaa
     1081 acgggaatac aatttcaaaa gtttaatact atatatcaat tattagcaat aaaaaatcaa
     1141 cagccagatt tattagaaaa ggcaaaaagc tttctaatga ttcctgatta ttttaacttt
     1201 ttattaactg ggaaaaaagt tacagaatat acaaatgcaa caactacaca attagtaaat
     1261 acaacaacaa ataattggga tgaagaaata ataaataaac ttggatttga caagatgtta
     1321 tttgctaaaa taaaaactcc caaaacaata ctaggtagtt taaagaaaga attagtagaa
     1381 gagttaggat gtgatttaga agtcatttta ccagttactc atgatacagg ttcagctttc
     1441 atgtctactc ctgtaaaaaa tagaagcatt ttattgagtt caggaacatg gtctttatta
     1501 ggagtagagt tagaaagacc aaagtgcaaa ttagaggctt tagattataa ttttactaat
     1561 gaaggtggat atgaatatag atatagattt ttaaaaaata taatgggttt atggatgata
     1621 caagaagcta gaaagtgtta tgacagtaag cactctttta cagaactagt agagcatgca
     1681 agactatcag aaggatttaa atcaatagta gatgtaaatg atgatagatt tttaaatcct
     1741 gaaaatatgg tacaagaaat aataaaatac tgtatagaaa ctaatcaaac agaaccacaa
     1801 acagttggag aagtagcata ttgtattttt aacagtttag caaaaagtta tagtaaatct
     1861 ataaatgaaa tagaagaaat cataggagaa aaattagaaa ctataagtgt atttggtgga
     1921 ggatgtcaaa atgaactgtt aaatgagctt atagcaaaag aaagtggtaa aaaggtatta
     1981 gcaggacctg ttgaagctac agcattaggt aatatagtat gtcaattaat tgctaagaat
     2041 gagctaaatg atttagaaca ggcgagagat ataataagaa attcatttga tataattcaa
     2101 tataattaat ctgaaaaagg agataagtga tggaattaaa gcaaaggtat gaagtagcta
     2161 aaaaagaata tgaaaaatgg ggaataaatg tagaggaagt attaagagtt ttatctaaag
     2221 ttaaaatatc tattcattgt tggcaaggtg atgatgtaac agggtttgaa ggaaatcaac
     2281 aaagactatc aggaggaatt gcatctacag gaaattatcc tggaaaggct agaaatcctc
     2341 aggagttaag acgagatttg gataaggctt taagtttaat accagggaaa cataaagtta
     2401 acttacatgc tatatatgca gaaactggtg gaagctttgt ggatagagat gagctaaaac
     2461 cagagtattt tgaaaattgg gtaaaatggg ctaaagaaag aggattggga ttagatttta
     2521 acccaacaat attctctcat ccaaaatcaa gtgatgggtt aacattgtca cacccagata
     2581 aaaaaatacg tgatttttgg ataaagcatt gcatagcttc tagaaaaata ggcgaatact
     2641 ttggaaagga gcttggtcag acttgtttaa caaacatatg gataccagat ggatacaaag
     2701 atataccaag tgatagatta ggtcctagaa gacgcttaaa ggaatcacta gaagaaatat
     2761 taaaggtaga gataaacaga gaatataatt tagattgtgt cgaatcaaaa gtttttggat
     2821 taggtgtaga atcttataca gttgggtcaa atgagtttta cttaaatttt gctagtaaaa
     2881 acaacataat ttcattatta gatacagggc actatcatcc tacagaactt gtttcagata
     2941 aattatcatc aatgctatta ttttcagata aagttgcact tcatgtaagt cgtccagtgc
     3001 gttgggatag tgaccatgta gttatattag atgatgaact aaaagaaata gccaaggaaa
     3061 tagtaagaaa tgatgctttg gataaagtaa taataggact agacttcttt gatgcaagta
     3121 taaatcgtat agcagcatgg gtaattggaa ctagaaatat gataaaagca ttgttatcag
     3181 ctatgttaat gcctcatgaa gaattaagaa aattacaaga cgaaggcgat tttacaaaaa
     3241 gattggctat aatggaagag ttcaaaacat atccaatggg agacatatgg aactattatt
     3301 gtaaaattaa taatgttgct gttaaagaag attggataga agtagtaaaa gagtacgaat
     3361 taacagaatt attaaataga aagtaggtaa gggtatatgt ctataatgga aatgaacttt
     3421 atcaaagatt ttatgaaaat aacttatgat gcttggttaa agggatggca tgagcgtaat
     3481 ggggggaata taacgtatag attaacacct gaagaaatat ctggaataaa agaatttata
     3541 acagaagaaa tagaatatat tccaattggt gtaacagtcg aaaatttagc taatgaatat
     3601 ttcttagtta caggaagtgg caaattcatg agaaatattt ctataaatac tgttcaaaat
     3661 gtaggaattg ttaaaataga tgaaaaaggt gaaaattatg caatagtttg gggattagat
     3721 aaggctaaac caacaagtga atttccatct cacttaatga gtcattctat aaaaaaagac
     3781 aaaacaaatg gattacatag agtaataatg cattcacata caactaatct catagcatta
     3841 acttttatac tgcctcttga gggtaaagta tttacaagag aattatggga gatggctaca
     3901 gaatgtccag ttgtatttcc agaaggaatt ggaatcgtac cttggatggt gccaggtggt
     3961 atagagatag caaaagctac acaaaatcta atggaaaaac ataacatagt aatctgggct
     4021 catcatggaa cgttctgctc aggagaaact ttggacttaa catttggtct tatggataca
     4081 gtagaaaaat cggctgagat attagtaaaa gtaatatcta tgggagggaa aaaacaaaca
     4141 ataacaggag ataactttag agacttatca aaaaatttta aggtttcaat atcagaggat
     4201 tatttgtact agataagata aaagataaac aaagctatga taatttatta tgtacaatta
     4261 ataaaacact atgagagagg aggtgattaa aagatataga aagtaaattc acttaagaaa
     4321 atagtttaag aataaactgg agtttataat aaaatattca acaatatatt tatatatata
     4381 aatatattgt tgaatataaa tataagatag taaattttat tgttagataa gggaaaacgt
     4441 tattaaaaat tgaattaatg agtaaataaa agtgggagga aataataaat gaagagagaa
     4501 gttacaattc aaaagcaggc tatatctata gttttgttta tggcagtttt attttgtggg
     4561 ctattacttc caatgattaa caataagtcg gaatatagtt tgccaataga gtcaataatg
     4621 tttatttcag catcaataat atcgctattt atagtggtta caacaaataa aacgtgggaa
     4681 gaagtatttg aaagtggatt agatggtgta aaaggagctt taccaggatt aataatatta
     4741 ttagtaatag gtccacttat tgcaggattt attatgagtg gattaatgcc tatgttagta
     4801 tattatggag ttaaattgat aaatccaaga tttgtatatg ctacagcatt aatattgtca
     4861 gcattgtttt cttcatttac aggaacttcc tggggatcag cagcaactat aggagttgta
     4921 atgatggggg taggtaatgc aataggagca aacaatgcta ttatagcagg tgctatagta
     4981 ggtggagctt actttggtga taaaatgtca cccctatcag atacaacaaa tatagctgca
     5041 atagcgtcaa aagttaattt atatgaccat gttgcatcta tgttatggac aacaggtcca
     5101 gcaactataa tagctttagt tatatatatt gcttgtgggt ttatattccc agcaacaaat
     5161 acaaatttaa atagtccaga aataactaga ttattggcag atttatcagg aatgttcaat
     5221 ttcaatataa ttttatttat acctctttta atagtattag taggttcagc aatgaaaaaa
     5281 cctgcaatac cagtaatgac aatagcagca tttttatcac ttgcatttgc agtaatcttt
     5341 caaggttttt ctttggcaga tgttcttata ggacttaaca gtggattctc actagatatg
     5401 gtaacttggc atgaatatgc aaaacctata gaaggacaag cttatatatt aggttttttc
     5461 caaaaaggtg gcttctggga attaggtaaa ttattgccaa tatcaacatc tatattattt
     5521 gttgttggag tacttggaag tattgatgct atgccagcaa cggtaaatgt gatatttggg
     5581 tgggttaagt ctagaacggc tataatatgt tcttcattat taacaggaat agcaatgata
     5641 gcaatgactg caaatggtat agcatgtagt tttgtaacag caggtatatt tggagataaa
     5701 tatgatgaga ataatataga tcgtagggta ctatcaagaa ctacagaaga tacaggtaca
     5761 ttgttagaag ttttatttcc ttggacacca gcagcaatat ttttcactca gacactagga
     5821 gttaatgttg gagaatacgc actttggtct atagtgaact ggggaactcc aattatagca
     5881 gtaatcttag cagttacagg gataggtaca tataaaaatt caaaggataa aaaacaatct
     5941 aataaagtat cataatgaat attttaactt tattacctaa catagcttat aaaaaataag
     6001 ctatgttaaa tgtatttttc atatataaaa atatttcata aggagattta gaacatggat
     6061 aaaaataaat ataagataga gacattaatg aatcatgtag gagaaaattt ggatggcgat
     6121 tatggagctg taaacccacc tatatatcaa tcatcaatat tcgcaagaag tacatatgaa
     6181 gatatagaag catattttga aggttcaaaa gatggatatc tttattcaag aactaaaaat
     6241 cctactaatg atattttaga agcaaagtta tctgcattag ctaatgctga agcaagtata
     6301 acctttggtt caggaatggg tgctatatca agtgctatac ttcattttgt aaaaaatggt
     6361 gaccatataa tagcttcaaa taatttatac aatcctacaa tgaactttat aacgaaggaa
     6421 ttgaaggaga aaggaaatat agaaaataca atagttgaag gtgatagaat agaagatttt
     6481 gaaaatgcta taaaacaaaa tacaacttta atatacctag agtgtccatc aacagcagta
     6541 tttaaaatgc aagatataaa ggcaattgta gcactagcaa aaacacacaa tataaaaaca
     6601 gtaatagata atacttgggc tacaccatta tatcaaagat gtattgagat gggaattgat
     6661 ttagaagttc attctttatc taaatattta aatggacatt cagatgtggt aggtggatgt
     6721 attattggaa gtaaaaaaga tatagaagaa ataagaggga gtgaattagc tttatatggt
     6781 gcaaaactag caccatttga agcattttta gttataagag gtttgagaac tttacacgct
     6841 agattatcag tacataccaa aaatgctaag gaagtagttg aatttttaga taatcattca
     6901 gcagttacta aggtatatca tccattatct aaatatttta atcaaaaaaa actagcaaag
     6961 aaacagttgc ttaattatac tgcgttatta agttttgaaa tagacgcaaa ggatgtaaat
     7021 gatgttaaaa aatttgtaaa taaattagat ttatttaaaa taggagtgag ttggggaggc
     7081 catgaaagtt tagtatttgc tccaaatata gctatattaa atgaaatgac tttagaacaa
     7141 gccaaagcat taaatgtgtc accatacaca attagaataa gtttaggtct tgaaaatagt
     7201 gaagatttaa tcaatgattt aaatgatgcg ctatccattt tataatataa taagcatata
     7261 tggaaaaaac atattaacta aatagtttat aatgtactta gcaaagaaaa attattatca
     7321 aagtaccata cataaacata tctatgtagt gtatttataa agtataggag tcaagtttta
     7381 gactgtagga tagatgtagg ctataaaatg aatattagat ataaaggggg aagatgtatg
     7441 aaaagagcat attattcaag tagtgtaaat gaatttatag tcaaagatat ttttactata
     7501 tttggtgaga taacgtgtaa tgatcaattt gcggctgatg acttacaaaa gaatacatgg
     7561 aaaaaggaaa ttggaatatt aaaaagagag ctctcaactt tcaaagatgg acacttatta
     7621 tttgagtata caataccacg aataggcagt agaattgaca atgtgttaat ttacaagggc
     7681 attgtttttc tccttgaatt taaagttgat gaagacactt atcctaagca tgcaattgaa
     7741 caagttacag actatgccct tgaccttaat tgttttcaca aagaaagtca tgataaactt
     7801 ttagtaccaa tacttatttg tacaaaagcc cctcaaaaag accaaagaat aagaatgatg
     7861 aaggaaaata ttttagaaac ttattgttgt aatgagttta atataggaaa atacataaag
     7921 aagatttgta ctacatataa tagaagttca tttaattcag acacttggat aaactcgtta
     7981 tacatgccaa ctccaacaat tattgaagct gcacaagctt tatatatggg ccataatgtt
     8041 gaagatattt caagaaatga tgcaagtgct aaaaatttaa atcaaacaac taaagctata
     8101 aataaaatta tagactatag taagactcat aacagaaaat caatatgttt tatcacagga
     8161 gttccaggag caggtaaaac acttgctggc cttaatattg ccattgagcg acaaaaagta
     8221 gatgaaaatg aacatgcagt cttcctttct ggaaatggtc cattagttga tgttttacaa
     8281 gaagcactag ctcgtgatga tgtaaccaga aatggaatac gaaaatctga agcgattcga
     8341 aaagcgaaag aatttattca gataattcat cattttagag atgaagctat atctgttgat
     8401 acaccaccta ttgaaagagt cacaattttt gatgaagcac aacgtgcgtg ggatgaacct
     8461 aatttgacta actttatgaa aagaaaaaaa ggcgtattag actttaatat gtcagagcct
     8521 gaatttctta ttagtatttt aaatcgtcat ataggatggg cgactattgt ttgtttgatt
     8581 ggtggaggtc aagagataaa taaaggtgag tcagatggaa tatcaggttg gtttgaatca
     8641 ctaagaaata attattctga ttgggatgtt tacatatcaa ataaaattat tgatgaagaa
     8701 tattctaaag gtaattcttt tgatgagtta acagaaggtg ttaattatag aataattgat
     8761 gatttgcact tatctgtatc gttacgttct tttagaagtg aaaatgtatc aacctttgtt
     8821 aaagctgttt tggatgtaga taaagttgct gcaaaggaat tatataatca atttaaaaaa
     8881 gattacccta tatgtgttac tagggatttg gaattagcta aaaagtgggt gagagataaa
     8941 tcaaaaggtt cacagcgtta tggaatgact gcaagttctg gagctaaacg gcttcgaaag
     9001 tatggagtgt gggtacaaaa taaaattgat gctacaaact ggtttcttaa tggtaaggat
     9061 gatgtacgag cttcatattt tttagaagaa actgcaacag agtttgatat ccaaggttta
     9121 gagctagact ggacaattgt ctgttgggat gggaacttaa gatttaagaa taaccatttt
     9181 gaatactata acttcaatgg aactaaatgg cagaacatca ataaagaaga taatatacta
     9241 tacttaaaaa atgcatatag agttcttctt acaagggcaa ggcaaggctt tattatattt
     9301 gtcccaatag gtgatgagag tgatattaca atgcagccaa atttttataa tggtgtgtat
     9361 gaatacttaa aagaaattgg aatagaggaa ctttaggttt aaagtaagga tgctattaag
     9421 tggattattt gtaaaaaatt atcttccttt ataatatatg agaaagtaaa actttttaac
     9481 taaagaatac attcaaaaga taataagaaa aaggagagta acaatttgag tttataaata
     9541 atcttaataa ttttcatcca atctaaaatt taaatgtggt ttttactata aacaggacat
     9601 tggattatat tgtaagttat atattagagt aattctaatt tagagtttaa aagagattac
     9661 aacttattag tttgtatatc tcttttttta ataaaatcaa taatatataa aactattggt
     9721 atatttaatg acaattcaaa tattgtatat aaccataatg aaagatatat aaataaaatt
     9781 ttaaatatac ttaaaaatat attaatataa tgtacataaa aaatatcatc aatatatgat
     9841 aagataaata caatagcaac attttattaa ttttttgggt ttttatatat ttaggtactg
     9901 gggtgatata gttgaaccaa gagatatttt ttaaaataat gaatgaaata ttggaaaata
     9961 ttgatgaagg ggtacatttt gttaataaag acgggaaaac tataatctac aataattctg
    10021 tacaaaaaat ggaaaaaatg aacagacacg atattttaac caattcattt tttgatatag
    10081 tcaacaaaat gaaaataaac aggagtacac tattagaagt tttggaaaaa agggaagtta
    10141 tcaaagacaa tattcagaaa tatctggata aaaatggaaa agagataaca gcaattaata
    10201 ctacaatacc aattgatatt aaaggcgagt ttgtaggggc acttgagata tcaaagaata
    10261 tgacaacgat agaaaattta ctaaatagaa attataaaaa aaatttagtc aataaagtta
    10321 gtagtagaca aaaaaagggt tatgaatttg atgatatttt aggggaaagt aatttgatta
    10381 aagaagcaat agctaaatct aagaaagctt gcaagtctga tgcttcagta tttatatacg
    10441 gtgaaactgg aacgggtaaa gagttattta gccagtccat tcactatgga agtaatagga
    10501 gaaactatcc atttatagca caaaattgtg cagctttgcc ggaatcacta ttcgaaggta
    10561 tcctttttgg aactagcaag ggaggattta caggagcaat agataggcca ggcttatttg
    10621 aacaagctaa caaagggaca ttgttattgg atgagataaa ttccatgcct attatgttac
    10681 aggctaaact attaagggtt ttacaagaag gttatgtaag aagggttgga ggaactaagg
    10741 atatacctat agatgtaaga attattgcaa cgacaaatga aagtccaaag gtaattttaa
    10801 acgagcataa aatgcgaaaa gatttatatt ataggcttaa cataattcat ataaaaattc
    10861 ctcctcttag agatagaaaa gaggacatac ctctattgtg caaaaaattt atttcaaagt
    10921 ataataaaaa gttgaataaa aaagtggtag gaatttcaga tgaagctatt aaaattttaa
    10981 aaaattacga ttggcaaggt aacataagag agctagaaaa cataatttat tgtactatga
    11041 gtatgatgga agatgaggta caaatagaaa caagtatgat taatctaaac gactattaca
    11101 atcttaaaga gaataaagag agtaacaatt attctttaaa cgatttggag gacaaaacac
    11161 ttagtaagat tatcagtgag atagaagaaa agtatatata tgaatcatta gaaaaggcat
    11221 cttacaatat aactaaagct gcatcaattt taggtatgaa taggcaaaat ttacagtata
    11281 aaataaaaaa atataatatc aaaatattat aaataagcaa gataaaaaac aaaatcaaag
    11341 taaaataaaa aagcaaaata gaggagcaaa attattataa aagagcaaaa tattttgctc
    11401 ttttttttat ttctcataag aaatattatt ttttatttat gaaaatacat aataaaataa
    11461 ttatagtttc aacgttccaa taaatattta ctttaatttt aaagttggca cgatttatgc
    11521 tttaactaat tattgagaat atataaattt ttaggagggt gtatatatga gaaaagtaag
    11581 agtaggaata tggggatttg gagctatggg aataggtatg gctaatatga ttctaaagaa
    11641 agaaggtata gaaatagtat ctgtttgtag tagaagtact tcagggaaaa gtatgtatga
    11701 tgttttagga attgaaagag gagaaagacc agaagttatt atcaataaaa attatgaaga
    11761 agtttttaga gaaaaaagtg tagatgttgt tttgttagca actgattcat ttactaaaaa
    11821 ggcttttgat aaaataattt tcttattaaa tagaaaaata aatgttatat caacagcaga
    11881 acaaatggca tatccacaag ctgatgatgc agatttagct aaaaaaatgg atgaagttgc
    11941 aaaggaaaat ggagtaagta tacttggaac aggaataaat ccaggatttg tattagattt
    12001 actagtattg gcactttcag gtacttgtga agaagttact agtataaaag ctaagagagt
    12061 aaatgattta tctccatttg gaaagtctgt tatggaggaa caaggtgttg gagtaacaag
    12121 ggaagaattt ataaaaggtg tagaggataa gactatagca ggtcatgtag gttttgtaga
    12181 atcaataaat atgatagctg atggattagg ttggaaatta gataagatag aacaaacaaa
    12241 agagccaata atgactacag tagatagaaa atctaagtac ggagaggcat tggctggaaa
    12301 tgttgctggt tgtagacaat gtggatatgg atatgtaaat ggagaagttt taattgaaat
    12361 ggagcatcca caacagataa taccagaagc agaaggtatt aaaacaggag actatgtatc
    12421 aataaaaggt ataccaaaca ttgatttaca aataaatcct gaaatacctg gaggagtagg
    12481 tacttatgca atgatagtaa actctattcc attaataata aatgctagac caggtcttaa
    12541 aactatgttg gatataccag tacctagagc aataatggga gatataagaa atcagataga
    12601 agttgaattg gaagaagaat caaaggctaa ttagtaagat aacagataac taattagtaa
    12661 gatagcaact agctaattag taagataaca gttagctaat tggtaaaata atgtttaatt
    12721 taaaataaca gtaaattaat tagtaaggca atagtcaatt aataagggga tgaaaaaatg
    12781 gatgctaaaa ttaatgattg ggttattata cacaatatag tattgactcc agaagaaaga
    12841 gctccacaag ttccagagga tactaagaaa gtatccttag aaatgtgggt taaaggtttt
    12901 atacaaagtg atgcttctat aggcgactta gtagaagtta aaacaattac aggcagactt
    12961 gtaaaaggta atttacttaa aataaatcca tattatactc atgattatgg taaatgtata
    13021 ccagagttac tccaaatagg aatccaagct aaagaaatat tatttggagg tgtatataat
    13081 gaataattca acatcaatga aagatatgag ttatcaagct gttatgggaa gaaataacga
    13141 aataatgaaa aatgcaatcg gattggatta ttcttctttt gaacaagagg gaatagggtt
    13201 tgattatgag aaaatgatga gtgaaacagg atatacatta caagatattg aagcaattca
    13261 atcacagtat gctgttggaa atactccttt aatagagctt aaaaatttga ctaaattagc
    13321 tagaaaatgt gctaaagaag gtaaaggagc tagaatattt gtaaaagatg aagcaatgaa
    13381 tgcatctggt agttttaaag caagaagagc agctactgca gtatatcatg ctaaacaaat
    13441 gggatataaa ggtgtaatag cagctactag tggtaactat ggtgcagctg tagcatcaca
    13501 agcggcaatg cagggattaa aatgtataat agttcaagaa tgttatgatt ctaatggtgt
    13561 aggtcaacct gaaattatag aaaaagcacg aaaatgtgag gcatatggag cagaagttgt
    13621 tcagttaact gttggaccag agttatttta tacgttctta gttttattag aagaaacagg
    13681 ctattttaac gcatcattat attcaccatt tggaattgca ggtgtagaaa ctttaggtta
    13741 tgagttagct atccaattta gagaaaagta taaaaaagac cctgatatag ttgtttgtac
    13801 aaatgcaggt ggtggtaatc ttactggaac tgcaagagga ttgataaaag ctggttctat
    13861 taatactaaa gtagtaggag caagtgtaga tttaaaagga ttacacatgg ctagtgataa
    13921 tcaatttaat aaaaaatcat ttacaacagg acatacaggt tttgggatac catactgtac
    13981 atggcctgat aggtcagatg tacctaggtc tgcagctaga ccacttagat atatggatag
    14041 atatgtatta gtaaaacaag gagaagtttt ttatacaact gaattattgg cacagctaga
    14101 aggtatagaa agaggtccag caggaaatac atctttagca gcagcattct ctttagctca
    14161 agagttagat gaagataaag ttatagtggt acaagaaaca gaatatacgg gtgcaggtaa
    14221 acatatatgt ccacaactta cctttgcaag agaaaatgga atagatataa aatttggaaa
    14281 tccaagagaa gaaattgcag gagtaaactt aatattacca gaaagaccag aattattgaa
    14341 atgtgttgat attgatatga ataaaataag aaagtcattt ataaaaaatt gtatatctaa
    14401 taatcacatt gataatattg ataagctaag taatcgagat atagaatttt taatggaaga
    14461 agtaaaaagt tcaagagatt ttgttgttga tgtattaaat aatttatagt aattattgtt
    14521 ttttaattta atataaaata aaaattcaga aaattcgatt aaatcaatac gaatggagga
    14581 atgttcatga aaaaaagaga ggatgatttt gaagttagaa gaaaacatct tcaagaactg
    14641 agtgaggatg agttaaaaga aaggttttgg agtttagcta cccagatagt agaaccaatg
    14701 atagaacttg gtaaaaagaa tacaacacca tcaatagaaa ggtctgtctt gttgagaatg
    14761 ggattttctt ctcttgaagt aaaacctatt ttagaagggg ttatggaaag aggtcttata
    14821 ggtaaaggag cagggcatgt agtttataaa ttggctaaat ctaaaaatat aacggttaga
    14881 gaagcaggtt tgttattagt taagggtgaa tattgggatg aagttacatg tttatttaga
    14941 gaaggggtag aatcatgtta aaggaaaata aaaaattgga tatagactat atattaaaag
    15001 atttggataa atataaacca aaacgaagag gttgggtttg gagagaacac ttggaaaatc
    15061 ttgaaatggg accttttaaa tataaagact gtacaaaacc tttaaagaag agtgtagggc
    15121 taccatcatc taagtatttc aataatatag accctcaacc aagtccagtt attactactg
    15181 aaatagcttc tggtagattt gaggatgata taagaagaat gagaatggca gctcatcatg
    15241 gagctgacca tctaatggtt atcagaactg ctggacaatc acattttgat gggcttatag
    15301 aaggaactcc acaaggtata ggtggagttc caataacaag aaaacaagta agagctcaaa
    15361 ggaaggctct tgattttata gaagaagaag ttggtagacc tattaattac cattcatatg
    15421 taagtggtgt tgctggtcct gaagtagcag taatgtttgc tgaggaagga gtcaatggag
    15481 cacatcaaga cccacaatat aatgttctgt ataggaatat aaacatggta aggtcttttg
    15541 tagatgcttg tgaggcaaag aaaataatgg cttttgccaa tatagctcaa attgatggag
    15601 cacataatgc taatgctact gctagagaag catggaaggt aatgcctgaa cttatggttc
    15661 aacacgcttt aaactctata ttctcagaaa aaataggtat agataaaagt aatatatgct
    15721 tatcaacagt tccaccaaca gcacctccag caccatgttt aaaaatagac ttaccttacg
    15781 ctgttgcact tagagagtta tttagtgatt ataagatgag agctcaaatg aatactaaat
    15841 atatggaatc atcaactaga gaagcaacag ttactcatgt attaaaccta ttaacatcag
    15901 tattaactag agctgacata caatcaacta taacacctga tgaaggtaga aatgtacctt
    15961 ggcatatata caatatagaa gcatgtgata cagcaaagca agctttagtt ggtatggatg
    16021 ggcttatgga tatgatagaa cttaaagatg tcggtgaatt aagagacaaa gcaagagaac
    16081 ttaaagaaag agctgtatta tatatggaag aaatatctga agttggtgga tattttgaat
    16141 ctgtggaaca aggatttttt gttgattctg gaaattatcc agaaagaaat ggtgatggaa
    16201 tatctagaaa aataaaaggt ggagttggtg aaggtactgt ttatgaaaga gaagaagatt
    16261 atttagcacc tgtaactgct cactttggat acaataatgt agctcaatat gatgaaaatg
    16321 ctatagataa tccatcaatg ttaatagatg gatgtacatt tgaaaatcca gacaaaataa
    16381 tatatattga tgagcttgac gattttgata atgtagaaaa tagactaaaa gaatcatatg
    16441 agtacagaaa tggaactaaa attaagcctg aaatggaatg gtgtgcagat ggaattgtaa
    16501 tgataacaat gatgcttcct actgataaaa gaacagcaga gtttgcagct ttggaatttg
    16561 tcaaaaaaat gaacttacaa gaaattgagg tcataagtag agaagttatg catgaatctg
    16621 aagggactag aatagaagtg aaaggaagag ttccgtttga tatagattta aacaatttag
    16681 taataccaga agaacctaag gtactaactg atgaagaaat aagagcagat atagaagaaa
    16741 aacctatgaa aatagtttca gctacggttg gagaagatga acattcagtt ggtttaagag
    16801 aaataataga tataaaacat ggaggtattg aaaaatatgg catagaatgc cactatctag
    16861 ggacttctgt acctgtagaa aaattggtag atgcagcgat tgaactgaat gcagatgcaa
    16921 tacttgcatc tacaataata agtcatgatg atattcacta taaaaacatg aaaaaattac
    16981 acgattattg tgtagaaaaa ggtataagag ataaagtaat gattgcgtgt ggaggaacac
    17041 aagtaacacc tgaaatagct gttgaacaag gcatagatgc tggatttgga agaaacagca
    17101 aaggcataca tgtagccact ttcttagttg aaaaaagaag agaaatgagt aataaataat
    17161 ggaaaatagc aaagtttctt taaaaataga tgtgttggta gctgaaattg gaagtactac
    17221 tacagtggta aatgctttcc acgatattaa tactaacaat cctatatttt tgggtcaagg
    17281 tcaagctcca acaacagtgt ttgaaggtgg cgatgtaaga aatggcttat caggtgctat
    17341 taaagattta gctaataaat tatctgtaga tgatatagag tataatgata tgtttgcaac
    17401 ttctagtgca gctggtggat taaaaatgac tgttcatgga ttagtctatg atatgacagt
    17461 aaaagcagct aaagaagcag ctttaggggc tggagctgtt attcgtcaaa taacatcagg
    17521 tagaattaaa agaactgatt taaataaaat aaaagaaata aacccaaaca taattttaat
    17581 agctggtgga gtcgattatg gagaaagaga tacagctatt tataatgcag aaatgatagc
    17641 ttctatgaat ctaggcatac cagttatata tgctggtaat gttgaaaatc aagaggaaat
    17701 aaggttaata tttgaagata ctaattataa gctttatata actgaaaatg tataccctaa
    17761 aatagattta ttgaatatag aacctacaag aagaatcatt caaagtgtat ttgaagaaca
    17821 cataactaca gcaccaggaa tgaaatacat taaagaaatg gttaatgaaa atattactcc
    17881 aaccccagga gctgtaatgg aagcttctaa acttttatat aaaaatattg gagatttatt
    17941 aactctggat gtaggtggag caactactga tgtacattct gttacagatg gaagtgacta
    18001 tattaataaa attttggtta atcctgaacc tactgcaaag agaagtgttg agggggattt
    18061 aggtgtatat gtaaatatga agaatatagt agaagttata ggtaaagaaa acttacaaag
    18121 tgaactatct atagatattg atgcagtaat agagaattat cctccaatac caaaatcaaa
    18181 agaagaaata ttatttgttg agaggttgac aaaagaagct gttgttaaag ctatgctaag
    18241 acattcagga aaaataagaa atatctatgg aacaactgga aaagtcaaaa ttgcagaagg
    18301 gaaagattta acagaagtaa gatatatagt tggtacaggg ggagcactta ctaggctccc
    18361 tagtagaatt gaaatattag ataatatgct caaatacaat aaaaataatg aattactatt
    18421 tccaaaggaa aaaacaaaga tattaataga taatgactat attatggcat ctatgggtgt
    18481 tttatctaaa aaatatgaag aagcatctct taagctcctg ttaaaaagtt tgaattttga
    18541 ggaggaaaga ttatgtatcc taggttagaa atagatatag aaaagttaaa gcataatgca
    18601 aaattaattt cgcagatgtg tcatgaaaga aatattaaga tatcttttgt tactaaatca
    18661 ttttgtgcac aaaaagaaat agtagaagaa atatgtagtg aaggaataga ccatatagca
    18721 gattcaagaa tacaaaactt aaaaaattta caagatataa atctacctaa gatattgatt
    18781 agaatcccaa tgttaagtga attagaagaa gttatcaatt attgcgatat tagttttaac
    18841 tcagaattag gaacaataaa gaaattaaat gaattatgtg aaagtaaaaa tattattcat
    18901 aagatagttc ttatgtttga cttaggcgat ttacgagaag gatactttta tgaagaagac
    18961 ttttttagta atgtaagaga gatagtaaga ttaaaaaata ttgaaataat tggtatagct
    19021 acaaacttaa cctgttatgg agctattata ccatctaggg aaaatatagg cagactggta
    19081 agtatagcaa aaagaatgga agaaaaattt ggaataaatc tagaaatagt atctggaggt
    19141 aattcaagtt cgattcatct gttgataaat aataatatgc cagaaggtat tactaatttg
    19201 agaataggcg agtccattct acttggtaga gaaacggcat acggtgaaaa tataaatgga
    19261 acatatcaag atgcatttaa attaatatgc caagtagttg aatgtaaaga aaaaccatca
    19321 gttcctattg gagaaatagg agttgatgcc tttagaaata aacctgttta tgaagataag
    19381 ggaatcttaa aaagggcaat catagctata ggtaaacaag atataaatat tgactcttta
    19441 attccaatag acactgatat aaaaatatta ggggcaagtt cagaccacat gatactagat
    19501 gtaagtaaca caaaatatga ttataaatta ggtgacaatt tagaattttt attaacttat
    19561 gggggtatca tgtcatctag tacgagcaaa tatgttgcaa aaaaaattat agtgtagtgt
    19621 ttaaataatt aggaggaaac tattttatga aagagcgaga atgtagaaaa gcaagtctgt
    19681 ttgatgcaat gataccaatt acttgtttga tattattttt aagtctagga gtattagtgt
    19741 atggttcatc acctcatgta ccactaatag gagcagcagc agtagcagga cttgtggctg
    19801 tatatagact tggatttaaa tggaaagagt tagaattaag tatgtttaat agtataaaga
    19861 tggcaatgca agcaatctta atcataatta taataggcgt acttatagga acttggatag
    19921 taagtggagt agttccatgt atgatttact ggggactaaa aattttatca ccaaatattt
    19981 ttttagtagc atcaacatta gtctgtgcaa tagtatctct tgcgacaggt tcatcttgga
    20041 gcacaatggg tacagtagga gtagctcttt taggaattgg tcaaagttta ggtatgccaa
    20101 ttggattaat cgtaggttct ataatatctg gagcttactt tggagataaa ctatcacctt
    20161 tatctgaaac gactaactta gctccagcaa tggcaggaac agatttgttt acacatataa
    20221 aatatatgct atattcgact ataccatcat tattaatttg tttagtaatt tatggtgtaa
    20281 ttggaatgaa gtattcaggt caagcactag atataaaaca aatagaatta attaggtcta
    20341 cattagattc tacatttaat actttatcac cagtattatt attagctcca gcaatagtaa
    20401 ttggattagt tgtatttaaa gttcctgcta taccagggct tatagtaggt gctgtattgg
    20461 gagtattgtt tgcagtaatt tttcaaggag aatctatgaa tactatatta gatgcagcac
    20521 aaaatggata tgtttcgtca acacaaatac aggaggtaga tgctttatta tctaaaggtg
    20581 ggattatgaa tatgatgtcc actgtatcct taacaatatg cgctttatct cttggaggta
    20641 ttttagaaaa aacaggtatg cttgaagtag tagcatcttc tttactaaga ttagcaaagg
    20701 gtgtatttgg aacagtatta tgtactatgg taacttgtac aattaccaat ataatagcag
    20761 gtgaacaata tctatctata gttataccag gaagaatgta taataaagaa tataaaaaga
    20821 gaggtataca tcctaagatg ttgtcgagag ctttagaaga tagtgggact ttaacttctc
    20881 cattagtacc atggaataca tgcggggcgt atataacagc aacattaggt gtatctgcac
    20941 taacatatgg tccatacgct ttattaaata ttataaatcc aatagtatct ttagtattaa
    21001 ttgcatgtaa gtttaaaata gcaagaatag aagatgaacc tgaaacttgt ttaaattttg
    21061 atgtttgata atagataact tatgtaagaa cgtaggaact aggggggtag cctgtgggga
    21121 attagtaaga catattttag tgtgcgaaat tctataaatc agtaatcttg tgactttagc
    21181 catgggaggt tcagattagt aaatttatag ttttatatta gtctatattt tgatatttta
    21241 attgtttgta taaatttttt atagaaaaag atttctttta gacttgccta aaaattaggc
    21301 aagtctttta agtatgttct gattataaga gcactgtgtg acaagaatga tgagattatt
    21361 tttgctatac tttcgatata agaaatggaa ggaggttatg ttttatattc atatgaaata
    21421 attagttaca cagtgaattt tcgtataatt tggtataaaa agattagaaa tgaaaataaa
    21481 gtaatggagg aataaaatga ctagaatatc caatataaca atagctgaac aatctgagta
    21541 ctgttttttg gcaatacgaa aaacaattga ctttatggta gaattttcag agttttcaaa
    21601 acaaagtttt gaaaaaattt ctaaatatct ggaggagaga ggaattttat cttcaggagc
    21661 accgattgta tgttttcata acatggattt agtaaaattg gatgttgaag tagggtttcc
    21721 tgtagccact tatatagatg ggaaaaatga gattctacaa aagattgttc ctgcacagaa
    21781 aataataact gcaattgatt taggacctta tgaattacaa gaccctactt tagaagactt
    21841 attttcatgg gcaaatagca atggatataa acttcatggc gatatatatt accagtattt
    21901 aaatgatatc aatcgtccag ctagtgaata tcttacgaaa atgatgttac ccattatata
    21961 gaaagttaaa gattatcgtt tgttagttga taattagaag aacatttact gctactacat
    22021 aaattttatc ctaaataata tcaaggtgat ttaatttatg tattctaatg taaccccaat
    22081 atattgttaa ggtctactta agatgctcaa aaggctatgc ttaatttatt gttatataat
    22141 ttgtagagtg gtataattta gaggtaatat tatatagata tataggggga aaatatgaaa
    22201 atatatgata tattaacacg tttttatatt caagatatag aaaaagccat acctttttat
    22261 gagaatcttt taaaagaaaa atgttctttg cgtttttcct ataaagaagt tgggttggaa
    22321 ttagcgcaaa taggaaatgt tttactatta tctggttctg atgatgctct taaaccattt
    22381 atagaaacaa aatctacttt tatggttgat tctgttgatg aatggagaag ttatctattg
    22441 gataatggag cagttgtagt aagagataag aaaaaagttc ctacaggata taatatgaca
    22501 ttgagacatc cagatggtac aattatagaa tatgttcaac atacaaagca ggattagtta
    22561 aaaacagatt ttactactga gcaactaatt agaatctatt cttagacgta caatttatta
    22621 tttttggtat taacagatta taagtcaaat tataatattt caaaatagaa tagaaattta
    22681 tttacataaa agagagtgtt taaaaatgaa ctttttagtt catgaagcac tcttttttgt
    22741 atgaaatcaa atacatcaat atataaaaca aactgatgaa gagcttaaat taaaataaaa
    22801 agattcataa caggacccca ttaaacttta agatagggta agtttgtaat catattaaat
    22861 aattgtaaaa agctgttttt agtcgtataa tataaatatt tatcatttaa ttaaggaatc
    22921 taaaaggaat ctaataagaa gaaaataata aataactttt tagatttact aaatgaaaaa
    22981 ttattgaaaa ttaaaggatt agagtcttta atcagaaagt aaaaagtaat atattaaatt
    23041 tatgaattga aaacttaaac aacaaatttg tttataaaat agcaagattc attaaatata
    23101 actaagtaat aattgatata atgttgcatg gataaaagga gggaagctgc aatgtcttac
    23161 aaagtaatta atgtcataac tgctattgac tatatagagg agcatttatc agaaaagctg
    23221 gatttagata tagttgctaa tgctgttcat tactcaaaat atcacctgca tagaactttc
    23281 acaacttctg ttggtctaac aatgcatgat tacataaagc ggagaaagtt gacagaagca
    23341 gcaaaattat tggtattttc aaagaaacca attattgaaa ttgcattgat agctggatat
    23401 gaaagtcaac aggcattcac ttctatattt aaagctatgt ataaaaaatc tccaaataaa
    23461 tataggaaag aacaagaatt ttatcctttg cagctaagat ttgttttgaa aaaagataac
    23521 ctattttcaa agaatgtact agagttggaa aaagaaatta aacttgcaag tatgtctgac
    23581 ataccattat ggatgaattt agtgagatta gtcatagatg gatttccaaa tttgcaagag
    23641 gaagaatata tatatcaatt agaacagtat attttagaag aaagagcttt aatattaaaa
    23701 cttaataatg tcgctattgc aaatatggtg tttaatagag aaactagaag tattgatttt
    23761 tttggaatac atccacagta tcgtaattca gacattgcac aagtgttttt gaagaaagtt
    23821 attgaagatt tcttgataga tacagatatt tcaattacta cctttcgaga gggtgacaaa
    23881 gctgatacag gacatagaga tatgataaag aagttgggct ttgcagaagc agagttgctt
    23941 gtggaatttg gatatcctac tcaaaaattt attcttcctt taagttaaat agcaagaatg
    24001 attaaaaact ccaaaacaca attaagtata atgattcagt gtaagtactt aattaacaaa
    24061 ggaggattta aatgtctgaa aataaacaag tagtaaattg cgatattatt gcatctaatt
    24121 ctaaacataa tatgtgtgaa tatgatgcag agttattaac tttttttcca gatgagattg
    24181 cacatctaat agagataaat aacaagctag acaatgcttt taagaaagct gaaaatcttg
    24241 ttgacaagct tgataaggat tatatggacg ctaaaatgta tatggtaaaa aatcgtggtg
    24301 aaatagaccc acatgaaatg tttcaaaatg aacaaggact aaaacaaata gataactatg
    24361 gggcttttat ggtaaaagtt cgagataaga ttaataaaat caaagattct ccatactttg
    24421 ctagaataga ttttaggctg aaagatatgg atgatgaatc taagtattat attggtagat
    24481 ttgcatttga ttatgaagat gagcttataa tacttgactg gcgttctcca attgccagta
    24541 tgttttatga ttatgaaatc ggaaaagctg gatatgatgc tccaatagga tgggtggatg
    24601 gagaaataac aagaaaacga caatttaaaa ttaaaaatgg aaaattagaa tatgcactag
    24661 aaagttccat taatatacaa gatgatattc ttcaaaaaga acttagtcac acttcagatg
    24721 aaaaaatgaa gtcaatcatt tcaacaatac aaaaggaaca aaatcaaatt attagaaatg
    24781 ataaagctga tacactgatt attcaagggg tagctggttc tgggaaaaca tctatagcat
    24841 tacacagaat tgcttttctt ctttatcgat ttaaagataa aatatcagca aataatgtaa
    24901 ttatcctatc acctaataaa gtctttggag actatatttc caatgtcctt cctgagcttg
    24961 gagaagaacc attatgtgaa ttaagttttg aaaatatagc agaggtacaa ttagatagag
    25021 ttattaattt tgaaagtgag aaagacccat tagaaataaa tgatgctaaa tggtctgaaa
    25081 gagtgcgttt taaatctaca cttgattttg taaaacttat tgatgattat ataaaacaaa
    25141 tgccaaataa gatttttatt ccaaaagact acacttttgg aagttttaca gctaaaagtg
    25201 actggataca gagtagattt gaggcatata acagatatcc agttaagaag agattagaga
    25261 atgtagctga agacattcat tataaatttg aatcagataa tattatggaa gaagatttac
    25321 caaaggtaaa aagtatactt aaaagtttga atggaatgtt gacaataaaa aatactctta
    25381 cactatataa ggatttcttt aaacaaatga atatatcaaa catgtttgtg atggtagaaa
    25441 agaaaacact tgaatggtca gatgtatacc catttatata tattcatgct gcatatgaag
    25501 gtatacaaga agataagatt ataaggcata ttgttattga tgaaatgcag gattatacac
    25561 ctattcaata tgctgtaata aacttacttt ttaagtgtaa aaagactatt cttggagatt
    25621 ttggacaatt ggttaatcct aatcatactc atacactaga tgatatgaaa cagctatata
    25681 atgatgggga attagttatg ttgaacaaga gttaccgttc tacttttgag attattaatt
    25741 tcgccaaaaa gattcaagac atatcttcat tagagcctat tgaacgacat ggtgaagaac
    25801 cagctttatt aaaatgtaat aacgatcaag atgaaataaa taagataaaa atagaaattg
    25861 aagaatttaa aaagagtgat aatgcgactc ttggtattat attaaagaca gataatgacg
    25921 ctaaagctgt atatgatgcg ttaagagaag aatatagtgt tcacttaata tcttctgaaa
    25981 gttctagctt tacaaaagga gtatccataa cttctattaa aatgtcaaaa ggtttagagt
    26041 ttgatgaagt tattgtacct tcagtaaata ataaaacata ttatagtgat tatgaccgca
    26101 gtttgttata tattgcctgt acacgagcta tgcataaact taagttgaca tatacttgtg
    26161 aattgactca acttatagat atgtagatta tatacttatt atgtaagtgt ttacttatat
    26221 aaatataacc tgtcaataaa attcattaat aaatcaataa aaacaatatt tgtaaaagta
    26281 aaagctataa attatatatt aaattttata atttatagct tttataatac tgcttatctt
    26341 aatcctccca atagcaaaat aattttaaac atgccatatt ttataaatgt aataacaatt
    26401 atacaattaa aataactttc ct
//
LOCUS       DAGSWI010000051        25626 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.051, whole genome shotgun sequence.
ACCESSION   DAGSWI010000051 DAGSWI010000000
VERSION     DAGSWI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.051"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(130..909)
                     /locus_tag="MJF80_001918"
     CDS             complement(130..909)
                     /locus_tag="MJF80_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBZ0280581.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LIQERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(902..2329)
                     /locus_tag="MJF80_001919"
     CDS             complement(902..2329)
                     /locus_tag="MJF80_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBZ0280582.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            2720..3442
                     /locus_tag="MJF80_001920"
     CDS             2720..3442
                     /locus_tag="MJF80_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0280583.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            3639..5306
                     /locus_tag="MJF80_001921"
     CDS             3639..5306
                     /locus_tag="MJF80_001921"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBZ0280584.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(5402..6721)
                     /locus_tag="MJF80_001922"
     CDS             complement(5402..6721)
                     /locus_tag="MJF80_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBZ0280585.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(6762..7508)
                     /locus_tag="MJF80_001923"
     CDS             complement(6762..7508)
                     /locus_tag="MJF80_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBZ0280586.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(7752..9761)
                     /locus_tag="MJF80_001924"
     CDS             complement(7752..9761)
                     /locus_tag="MJF80_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0280587.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKRNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(10199..11632)
                     /locus_tag="MJF80_001925"
     CDS             complement(10199..11632)
                     /locus_tag="MJF80_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBZ0280588.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYKNVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYAFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(11648..13072)
                     /gene="ascB"
                     /locus_tag="MJF80_001926"
     CDS             complement(11648..13072)
                     /gene="ascB"
                     /locus_tag="MJF80_001926"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBZ0280589.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEDGRGLANVDVTPQGKDRFPV
                     LAGKMKMFECDDEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGVE
                     DVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKGKVKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMLAAGNTYPNTCNPLDIWKSIEKDRENYFFIDVQSRGEYPTY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPKVNILTEGNVFASL
                     KNPYLDASEWGWQIDPLGLRITMNSLYDRYQKPLFIVENGLGAVDNPDENGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNDGNG
                     TLKRSKKKSFAWYKKVIESNGEEL"
     gene            complement(13159..15075)
                     /locus_tag="MJF80_001927"
     CDS             complement(13159..15075)
                     /locus_tag="MJF80_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBZ0280590.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(15149..16009)
                     /locus_tag="MJF80_001928"
     CDS             complement(15149..16009)
                     /locus_tag="MJF80_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBZ0280591.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(16360..17547)
                     /locus_tag="MJF80_001929"
     CDS             complement(16360..17547)
                     /locus_tag="MJF80_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBZ0280592.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(17561..18952)
                     /locus_tag="MJF80_001930"
     CDS             complement(17561..18952)
                     /locus_tag="MJF80_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBZ0280593.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(19547..21247)
                     /locus_tag="MJF80_001931"
     CDS             complement(19547..21247)
                     /locus_tag="MJF80_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBZ0280594.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(21219..22622)
                     /locus_tag="MJF80_001932"
     CDS             complement(21219..22622)
                     /locus_tag="MJF80_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBZ0280595.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(22886..23377)
                     /locus_tag="MJF80_001933"
     CDS             complement(22886..23377)
                     /locus_tag="MJF80_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBZ0280596.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(23403..24215)
                     /locus_tag="MJF80_001934"
     CDS             complement(23403..24215)
                     /locus_tag="MJF80_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBZ0280597.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(24228..25187)
                     /locus_tag="MJF80_001935"
                     /pseudo
     CDS             complement(24228..25187)
                     /locus_tag="MJF80_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(25305..>25626)
                     /locus_tag="MJF80_001936"
     CDS             complement(25305..>25626)
                     /locus_tag="MJF80_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBZ0280598.1"
                     /translation="CIEDLQVKNMIKNRKLSRSISDVSWSEFIRQLEYKANWHGRQIV
                     KVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLIT
                     IQNK"
BASE COUNT         8212 a         4765 c         2818 g         9831 t
ORIGIN      
        1 tgtgtacttc ctatattgat agagatgatt caagttttta ttttgataca tctctatcaa
       61 tattttttta gaaaatacac atttttttca aatacttatt tttaattaaa actttaggta
      121 atctattctt tatatataat tattagactt gaaaatatct ttaaaatcaa ccaattcaat
      181 attatttctt tttaaccaca ttttaacttc cttatcacat agcattgcat gttcatatac
      241 tctctcttga ataagagaag atgattttat aagactataa tctataaatc ctggatgagt
      301 aaccatcaat gttatatctt tatctaaatt tatatatttt tcttcaaata atttaacaaa
      361 agatttattt aacttgtaaa aatcattctg aggatattta cttggcatgc taatacatga
      421 atccaaataa tagggacaac ttggtatacc gtattctttt gcagcatcac acattgctga
      481 tattgataca tcttcttcta taccatgtat atcaatatac tcaggcaatt tactcatata
      541 caacttgtaa gtttcaattt gagcacaaac ttcttcatat acttcttcat aattaaaaac
      601 ctctttacct gtcagtagtt gcgttcttct tattttgcta gaaacaaatt ttccaccttc
      661 atctaacaaa ttctttatct taatatgttc tttgcaacaa ggttctccta ttactaaatt
      721 aacatgtaaa ccaaaacata catcatcatt ttttaccatt tctatagctt gtcttgaaaa
      781 aggcatattc accatgattc cagcacttgt aacaataccg ttcttataag aagatattat
      841 ccctagtgta acaccttctg aatatcctat atcatctgcc ctaactacta atttcttcat
      901 tttatgcatc tgcctgcttt ctcaactcat caagttgcat ttccatctca tcctttactt
      961 taacaacatc cgttcctatg atgatttgaa ctgatgtatt actcaattta attacccctt
     1021 tggctcctgt ttttttaata ttatcatcat tcaaaatagt ggcatccttc acttctaaac
     1081 gtaatctcgt catacaatta ttaagagtca ctatattttc ataaccacca caatttttaa
     1141 ttatctgttt agccatatat gaaaaattac catgagaaag ttttaaattc ttttcttttt
     1201 cactaacttt atcacctatc tctatctcaa cttcacgtcc tggtgtttgc acattatctt
     1261 ttattattat atatttaaat gttacataat ataatacaaa gaatacaact cctacaggta
     1321 taactaacca cgctttatct ccataactaa aacttaagac atagtcaatt atacttgctc
     1381 cattaaatga acccaagcga gcacccaata aatatagtac aactccacct agaccagaat
     1441 aaaaagaatg aattatatat aactttggag atgcaaataa aaacgaaaat tctattggtt
     1501 cagttactgt tgccataaaa gaagtcaatg ctccagcggt cattgcacct tttacttctt
     1561 gcttcttttt atcttgtgct gttttatata tagcataaga aatagcaggt attgaaaaca
     1621 tcataattat gtaaaatcct gtccagaaga atcctgcatt tgggtctcct gccataaatc
     1681 taggaatttc tcctcttata acttctcctg ttgatgttgt atattcaccc atttcaaata
     1741 atatgtaagc atttaaaact tgatgtaacc cagttggtat tagtgcacgg ttacagaata
     1801 aaaacaagaa tattcctatt gcaccagctc ctcccaacca aaaaccaaat tgattcattg
     1861 cagcttgtgg atatatccat aataaactaa atatatatga taaaattaaa gcaaccactg
     1921 gtgatagtgt aattacacat ttttctcctg caaagaagct taaaaatgct ggaactttaa
     1981 ctttatatgt tttattgtaa gtccatgcta tacaagctcc aacaattatc ccaccaaata
     2041 cacctgcatt taatgtatca ttcaataact ctaaatttga cttaaatatc atatatgcaa
     2101 ctacggctgc aaccattaca cttgtcttgt ctttagactt tgagtaggca ctagctgctc
     2161 ctaataaaaa taagtagtcc acatttgaaa atactatatt agatatatta gatatagctg
     2221 gcacattgag catatttgga ccaaataata atcctaatat acctgctgct ggcattacag
     2281 ctattggcac cagcatagtc tttcctaaaa gtgacagttg ttgcatcatt ttgtttaaga
     2341 atctcttcat atccaatccc ctttctattt cggtttaatt ttttgtgaaa acgatttatt
     2401 cacattttat attttaaata ttatatttgt ttaaacaaat tgtcaatatt ttttattcat
     2461 tttattttta tcatttagtt taacatataa atccttttaa gtgtattgct atatctagtc
     2521 tattttttta attcattttt tttattttaa aaatatgttt aaccatgtat ttgaaacaac
     2581 ccacaaaata attctatttt gtccttttta tggttgatat gattctattt tcactataca
     2641 tgtctaaaca catttgatat aatttgttca tatcagttca ataatattac taaagacctt
     2701 taaggaggga tagaggtata tggctaaaaa taaattttca gagatatatg aagtattgaa
     2761 agaggaaata cttgatggta aatatacaag taatatgatg ctgcctactg aacttcaact
     2821 tattgaaagg ttttcatgta gcagaaacac tgtacgtaga gcaatttctc aattaaacac
     2881 agaaggatat gttcaaagta taaaaggcaa gggtgttgta gtgttagaaa atagttgttc
     2941 taatgatttt tttctaaata tgcacaattt caaaggtgta gaatcgattg tagaagataa
     3001 aaaggttaat actgctacta gtgttctcca cttcagcaaa atcttaattg ataataagtt
     3061 atctaaaaag acaggtttta aagttggtag tgaagtatat taccttcaca gactaagata
     3121 tattgataac atacctaaaa ttctcgacat taactatttt ctatgtagta tagttaaaga
     3181 ccttgatgtt tctatagcac aaggctctat ttataaatat atagaagagt gtctaggtac
     3241 aaaaattgtt tcatccagaa agatatttaa aattgaaaaa gctacagagc ttgagttaaa
     3301 aacgctacct ctaaatgatt ataattgtgt tggtgttatt aaaaatagtg tatatactga
     3361 tgatggaaag ctttttgaat atactgaatc aaagcataca cctgaaacat ttgtattcat
     3421 ggatgttact caaagatatt aaaaaaatta tgtttaatta aacataattt ttttacgttt
     3481 agacacttta tcccatacta acctcactat tctatataca attatatgtt tatacaaata
     3541 attatttatt gtatatttta gctttaatat tagtttaacg cttgacaatt tgtttaaaca
     3601 tattataatt aacttataaa ttacaggagg tatcaagtat gaaaaattgg tggaaaaaag
     3661 caactgtcta tcaaatatat ccaaaaagtt tcaaagatag caacaatgat ggtataggtg
     3721 atataaatgg tattatagaa aaattagatt atctgtattc tttaggtgtt gatttattat
     3781 ggttgactcc tatgtacgtt tctcctcaaa gagataatgg atatgatata gaagattatt
     3841 ataatataga cccaaaatat gggacaatga gtgactttga aaaactctta aaagaagctc
     3901 ataaaagaga cataaaaata atgatggata tggtcctaaa ccatacatct actgaacata
     3961 aatggtttaa agaatctaaa aaaagcaaag acaatccata tcgtgattat tatttctgga
     4021 aagatgctaa acctgatggt tctgttccta ataactggat atccagattt agtggaactg
     4081 cttggaaata tgatgaaacc actaaccaat attatttaca tttatttgaa gaaactcaag
     4141 ctgatttaaa ttgggaaaat gaaaaggttc gtgaagaatg ttacaaaata ctagaatttt
     4201 gggctgataa aggtattgat ggttttcgtt tagatgtagt caacctactc tccaaaacac
     4261 ctggattacc tgatgaccct ataacaggac caaaaggaga tggaagaact cactacgctg
     4321 atggtcctcg tatacacgaa tatttacata atatgaatca aaaagttttc aaacctaaaa
     4381 atatagttac tgtaggtgaa atgtcatcta ctacacctga agagtgtatc aactatacta
     4441 gagaaaatcg agaagaacta tctatggttt ttagcttcca tcatatgaaa acagattata
     4501 aaggaggcgc aaaatggact aatgaaatgt tctctctgga taaattaaaa aaagcacaat
     4561 ctgactttca atataaaatg tatgaaggaa agggatggaa tgctctattt tactctaatc
     4621 acgaccaacc tagagcttta tctcgatttg gagatgatag attttttcgt caagaaagtg
     4681 ctaagatgtt agccataaca ttatttggtc tacagggtac tacatacata tatcaaggtg
     4741 aagaaatagg tatgacaaat gcctacttta ctaaaataga tgaatatgat gatgctgaaa
     4801 gtaagaatgc atactatcat atgattaaat ctggtattga tgaaaaggag gcattgctta
     4861 tacttcaaca aaaatccaga gataatgccc gtactcctat gcaatgggat aatactattt
     4921 ataaggggtt ttctaatcat aaaccgtggt taaaagttaa taatgacaat attagtgtag
     4981 aaaatgagct taatgattct cgttcagtat tttatactta tcaaagatta atcaaatatc
     5041 gtaaacaata tgatattttt acagaaggta cttatagatt gttagatgaa aatcataaaa
     5101 atctatttgt ttatgaaaga gagtataaaa accaaaatct tcttgttata agtaatttta
     5161 ctcatgataa tgtagtatat aaattacctg aaacttattt gaataaatta aactacacaa
     5221 tacttgaaac caattacact agagatataa ttgaaaataa aatggaaata aaaccttacg
     5281 aaagtataat gatatattat aaataaagtt atttatgtaa aaatcggtat aacaccctgg
     5341 tgttatacca attttactaa attcaaagta atatttatag aatatctata gattacaata
     5401 attatttgac ttctacatca ttatatttct ttgaatatac tttagtcata tagaaatata
     5461 taggaactac aattatcatc ccacctaatc cccaaactat cttcataggt tcatcaatac
     5521 ctgcttttat cagtaataat atacttataa taactgcaaa tattggtata attgctcctc
     5581 caggtattct aaaatttaca tctttaccag catacttctt tctcaatatt agtacagcta
     5641 aacatgtagg aatatattga ataaatctag atacaacact tatttgagat aatgttgtga
     5701 atgaacccga ccatgctatt agtaaagttc ctatgacaga tactataatt gctatataag
     5761 gagctccatt tttattagtt tttcttatag acttaggcat taatccttgt tcaaccaaag
     5821 cagcagcaga ccttggtgta aatatggatg atgcaatatt tatacctcca acagatacta
     5881 tagttcctat tgtaattata tacccaccta taggacctaa catcttaatg gcagcatctg
     5941 caacaggagt agctgaccct gctaagccat tacccaacac ccctatactt attcccagta
     6001 taagcatata tattactgat actacaaaca ttaccattac caatgcttta ggtacatttt
     6061 tcttagggtt ctccatatct tttgctgcaa cagctaaaga ttcaaatcct gtaaaagcat
     6121 aaaataaagt tatagatact gctacaaaag ctgatgacgc actttgacct ttttgtatta
     6181 ttacaaatgg ggtaaaatta gaacccttta taaagaataa tcctacagct ataaataaaa
     6241 ctattggtaa taattttcct atagtcatga tattatttaa gtgcttagat tgtttcattc
     6301 ccataaaatt tataatccca agtcctacaa ctaatatacc aattattata tttttagtaa
     6361 gtggttctgc tgcttgtggc catacagatg atagtgttac agcaaagaaa tttgccatag
     6421 tagcccaagc aatcatacta actatccact tcataaatcc aacttcaaat ccaacaaaat
     6481 ctccaaatgc ctcttttgca taaagatagg gccctccatc agtatcaaac caactacctg
     6541 cttcagcaaa acataaagca atagaaacag ctaaaagcat acaaaaaact aatactaata
     6601 tacttgctgg acctatatct gtataagcat caccaggtaa cccgaatata cctgacccta
     6661 ttattgcatt gattcctaat agtacaatac tccaaaaacc taacttaatg cctttttcca
     6721 ttgttattcc ccccaaagcc aacttaatat atttatattt tttatttaga tgcttcttta
     6781 actattgcca taaatatttt tttcatatta tcataatctc tagtcatcat ttctggatgc
     6841 cattgtatcc ctattacaaa ctcacttcct tccttttcta tagcttcaat taaaccgtct
     6901 ttagatgtag ctgaaacaat atatcccggt gctactttgt tgacaactaa gtgatgaaaa
     6961 ctattaacta aaacttcttt ttctcctaat atttcatata attttgtccc ctcctttatc
     7021 tgtactgtat gtgtaggtac attagataga tgttgctgat tgtgtttaat ataagcacct
     7081 tcaattaaag ataaatcttg atacaaacta cctccttcag ttacatttat aatttgttct
     7141 cctctacata tacctaaaac tggttttttc atatctaaag catgttttaa taatttcata
     7201 tcaaaactat ctctctttgg tgatattgcc cccaacttat tatgaggttc ttctttccat
     7261 atgagtggat ttacatcttg tcccccagat agtataagcg catctatatt agatacctgc
     7321 tcctttataa tctcatcatc atatacaatc ggtacaatat atggtatagc tttacacata
     7381 actactgatt gtatataatc attatttaca taagcccttt catagccagg aaacattcct
     7441 ccttcatcaa ttagtaaatt acctgatatt cctattattg gatatttctt tttagccaat
     7501 ttcttcaccc cttaacttat ttgtgaacaa taaaaaatgc tacttctctt aaagagcaag
     7561 tagcatacca agattttatt tatttgaaca ttataattta ttttagaaat actttttttg
     7621 aatacgagta atataaataa ctttatttac ttatatttta ttttaatcct gtttagtttg
     7681 acttttttaa attgttttaa gtacaactaa taggtaaaat aggcatttag attttccttt
     7741 ttatcaacct attaaatact gtttaatata ttttttcccc ttctatttaa ttcacttccc
     7801 tttctaccag attcacatat tatcaaatcc agctctttta acatattaag tattcccctt
     7861 acctcacctt ctgttatatt gtagcctaat tcatttattg aattaacaat accatttcta
     7921 cctatatttt tattaagatt attggcattt tttaaacact ccattactaa ctttactttc
     7981 tctatagatg ttctatcctt taatatactt atttctttac taaaatcttc agttatattt
     8041 aataagttat gaggtaaatc atttattaaa ataacatcct cagaactcat aatgttaata
     8101 tacatagcag tattttttaa ttctcttata ttacctggcc attcatatgc attaataatg
     8161 tcttgaacat cttgactaat tgctctcttc cttttcataa aatgctttaa cataacttct
     8221 atatcttctt ttcgctctct taaaggtggt atatttatgg gcaatacatt tatcctatag
     8281 tataaatctg ctctaaatct atctcctttt atcatttcta ataaattttt atttgtagcc
     8341 gcaattactc taacatctat atctattaca ttttctcctc caaccctcat tacttgattc
     8401 tcttgtaata ctctaagtaa tttgctttgt aagtatatag gcatgtctcc tatttcatcc
     8461 aaaaatatag ttccattgtt agcctgttca aataatcctt ttttacctcc ttttaaagct
     8521 cctgtaaaag caccttcttc atatccaaaa agttcacttt caagtaaatt ttctggcata
     8581 gctgcacagt ttattgctat aaatggctga ctatgtcttg gtgatgcatt atgaatagac
     8641 tgagccatga gttcttttcc cgttccactt tctcccgtta taagaattgt ataatcagat
     8701 tcagctattt tcatacctaa tttcttagtt ctttccatct tcgttgaaac tgtttttata
     8761 tcatcaaaag tatatctagc aacttgtccc ttttctctaa gcttctttga taggttttgc
     8821 tccaattttc ttatgtaagt tatttcttga atacaaaaat aatatccagt aactcttcca
     8881 aaactctcta tattatactt atttacgttt aagtacttat tattaaattt tataacttca
     8941 tcatgtgcct tactcaaact aaaaatcttt ttaatattct cattctctaa ttcatctata
     9001 tttttcccta taatattact ttgaatatct agtatatccc ttaaactatt attgcaaata
     9061 attatgtttc tcttatctga taccattgcc atacctaagt ttgataaatt tattatagaa
     9121 tttagttcat ctgcttttat agtaagttct ttgtatttag tatttattcc tgaatataaa
     9181 ctaattattt catctgtata cttaattagc ctctcagtaa tttctttatc atccagtttg
     9241 agttttgcta ttatttgaat aaacgttgaa ctatcaatat acctatggcc taaatcaata
     9301 acttcttcta tatattttgg aactaaactt gactctcctg gcgttatagc tatcttaata
     9361 tttctatatt cttcattggg attgtatggt ataaaattca gatgatttat tcctatattg
     9421 tataaattag atattgtttc caatacagta tgcttattat cgtttactac taatacatct
     9481 attccttctg gcaatgaaaa taacttatat atatcctttt gctttatgct cctatttata
     9541 gttattatct ttgagacatc ttctacatat ttttttattt ttaatactct atcatctatc
     9601 attaccaata ttatgtcatc ttttattaat tccttctctg ttattttatt taaataataa
     9661 ttttttatca ttactttatc tttaaatatc atttctagat ttttatttaa gaaacattct
     9721 aatttagaca acccatcagt tactactgct attgttttca tttgattact ccttttaaaa
     9781 tatactctac tcttttataa tatacatctg attttcactt taatagcaca aaaaattgta
     9841 catttagtat actaacatat tcctttatta gttttatgtc cctaaatatc atagttgcaa
     9901 tatctatcat atttcctaaa cttctataga gcaaaaattg tgccaaatta ataattttct
     9961 taattttatt ctaaagttcc tcaatagtat ttacagactt agcacttata gcttataagt
    10021 ctttatatta ttaatctatt tttataggtt aattgtacaa atcatatatc acttttaccc
    10081 attaaatgca ttataaaaaa gatggaataa cgattcgtca ctccaccttt tttataataa
    10141 aaaattgtat atgtaaacac tatttactac tcttctaagt acaaacagta tctacatact
    10201 ataacctttc accattagat tctattactt gtttatacca gtcaaaactc tttttcttaa
    10261 tcctagacaa gtctccactt ccatcatcat acttatccac gtatataaac ccatatctct
    10321 tggccatctc accagtagat gcacttacta aatctataca tccccacgga gtatatccaa
    10381 ataaatcaac accatcttct acagcttcct tcatttgctc tatatgtgct tttaaatact
    10441 ctatacgata gtcatcattt atcactccat tagattcttt tttatcataa gctcctaaac
    10501 cattttcaac taccatcata ggtattctat atctatcata aatttcattt agtgcatatc
    10561 taagcccttc agggtctatt tgccatcccc attcactaga cttaagatat ggatttttca
    10621 ctccacctaa tatgttgcca cttgtattat cttctttaga atcactactt acacatgtag
    10681 acatataata actaaatgca taaaaatcta cacaaccttc ttttaaaatt tctaaatctc
    10741 ctttttccat ttttatttca atattatttt ctccaaaata tctattcata taagttggat
    10801 attcgccacg cacttgtaca tctccacaga aataattcat tactctacta tctctttgag
    10861 ttttaactgc atcatctgga ttacaagtat aaggatacat tgttgcaaat atttgcatac
    10921 atccaacttg ataatttgaa tctatttcat gagctaactt aacagcctta gcacttgcta
    10981 caaattgatg atgtaaccct tgaaaacgaa gttgaggtat atctacttgg tttataaaat
    11041 ctgtagtccc ttcatttaat attccttgtg ataagtagcc tcccataggc attgtagctg
    11101 aatttatttc attaaatgtc agccaatatt ttacttttcc tttatatctt ttaaatattg
    11161 tttcacagta attcatatac aagtctatta catctcttga aacccatcca ttgtatttct
    11221 ctgttattgc aaatggtatt tcatagtgag atattgttat taatggttct atattatatt
    11281 ttaaacattc atctatcaca tttttataaa atttaagtcc tgcttcattt ggttctttct
    11341 ccattccagt tggaaatatt ctagtccatg ctattgaaaa tctaaatacc ttaaatccca
    11401 tttctgcaaa taaagctata tcttctttgt atctgtgata aaaatctatt gcttcatgac
    11461 ttggataaaa tgttcctttt tctataactc tcgttattct tttacttact ttttgagaac
    11521 ctccagtaca catatctgaa caagaaggac ctttcccatc taaattccat gctccttcac
    11581 attgatttgc tgctgttgct ccaccccata aaaaatcttt tttaaatgac atacctatat
    11641 cctcctatta taattcttca ccatttgatt ctattacttt tttataccag gcaaaactct
    11701 tctttttact gcgttttaaa gttccatttc catcattatc tttatccaca tatataaatc
    11761 catagcgctt cttcatttct ccagtacttg cacttactaa gtcaatacat ccccatggtg
    11821 tgtaccccca taaatcaaca ccatctaact caacagcatc tatcatggct tttatatgag
    11881 cacgtaaata atcaattcta taatcatcat ttatatttcc attttcatct ggattatcta
    11941 ctgctcctag tccattttca actataaata atggtttttg ataacggtca tataaagagt
    12001 tcatagttat tctaagccct aatgggtcta tttgccatcc ccactcactt gcatctagat
    12061 atggattttt aagacttgca aaaacatttc cttctgttaa aatatttact tttgggtctg
    12121 cacttgtaag tcttgatgag taatatgaga acgaaataaa atctactgtg ttctctttaa
    12181 gtatcttttt atcatcttct tccatcacta tatttattcc ttttctctcc aattctttta
    12241 atgcatatgt tggatattct ccacgagatt gtacatctat aaaaaagtaa ttttccctat
    12301 ctttttctat agatttccat atatctagtg ggttacaagt attaggataa gtatttccag
    12361 ctgctaacat acaccctatt ttaaattctt gatttatgtt atgtgctagt ttagtaacta
    12421 acgcactcgc aactaattga tgatgtgcag cttggtattt tatttgttct tcattttctc
    12481 cctcttcaat atagagacct gcacctataa atggtaaatg aagtatcatg tttatttcat
    12541 taaatgttag ccaatacttt accttacctt taaaccttgt aaaaataact tcacagtatt
    12601 ttacatataa gtctactagc ttacgatttc tccatgctcc atattttttt accaaataca
    12661 tgggaacatc aaaatgacag attgttacta ctggttctat tccatacttt aaacactcat
    12721 caaataaatc ttcatagaat tttagtccct cttcatttgg aacatcctca actcctgttg
    12781 gaaatatcct agtccatgct attgacatac gatagcattt aaatcccatc tcagcaaata
    12841 attttatatc ttctttataa ttgtgataca tatctattgc ttcatgagct gggtaatagt
    12901 gttcatcatc acattcaaac attttcattt tcccagccaa aactggaaat ctatcttttc
    12961 cttgcggtgt aacgtcaaca ttagctagac ctctaccatc ttctttatat ccaccttcac
    13021 attgatttgc tgctgttgct ccaccccata aaaaatcttt tctaagtgcc ataattctac
    13081 ctccaataat ttatttatag taactttatt ttaaaataaa aatacatata tataattctt
    13141 aattaataga actctctttt acgctaaaac agttatcaat ttctgactat tatttacatg
    13201 cttttgtgta ttttctacaa tatccacata atcagaataa ttagtaacaa ctactggtgt
    13261 ttctaagcaa tatccttctt ttgaaatact ttctatgtca aaagttatta atgtctgacc
    13321 ttttttaatc ttatcccctt gattaacaaa agatttgaaa tatttacctt ccaactgaat
    13381 agtatttaac cctatatgta tcaatagttc acaaccatta tcagatataa ttcctattgc
    13441 atgttttgtt ggaaataaag tcattacagt cccatcaaat ggtgccacta cttttccttc
    13501 tttaggtgtt attgctacac cttttcccaa tatacctgtt gaaaacgctg catcttgtat
    13561 ctgctccaaa gcctttactt caccttttat tgggctatat actacttctc ttttgttttt
    13621 ttctataacg ccttgcccat tatcatcttt agaaatttct actatagcat catctttcca
    13681 aaaaaataat gttaaaacaa atcctactac aatagctact attattgcta ttatagttgc
    13741 ataaacacct ttcatatctc cagttgttgg atttatataa ttaggtatac caaatattcc
    13801 aagtccaccc attacataac tttttacatt cattatcccc attacagctc ctgccacacc
    13861 accacctaca cagctatata tgaatggtgt ttttcttggt aaagttattc catatattgc
    13921 aggctctgtt acaccacata tccctgaaat aactgcaggt atacacagct cttttatctt
    13981 cttatctttt aatttaaagt acattgctgc tactactgct gtttgtgcaa aagatggtac
    14041 aaatgctccc actaatattg tgtcaaaccc catagtcata aggtttgaca tagctattgg
    14101 tactaatccc caatgtagac caaatattac aagcacttgc cacaataaac cagttaatgc
    14161 accacataaa attggtgaaa agttaaataa agctaaaaat ccatttccaa ctatattaga
    14221 agcataggta gctataggcc ctataaccat aaatcccaat ggtaaagaaa ctaataaaac
    14281 tgccattggt acaaagaaat tctttactac atctggtatt attttagcaa acactttctc
    14341 caacttagct gctaataata ctattaaaat tactggtact actgttgaag tgtagttcat
    14401 tgctattact ggtaatccta agaaagttat atatactggt gattcaaata ttgttccagt
    14461 aaatagattg tataaaggtt gacctgtaga tgctagagta tctaattgaa tacctgaata
    14521 gcataaaact gcacctataa gcattccaat aaatggtttt aatttgaatt tcttagcagc
    14581 tgtatatcca agaattattg gcatatactg gaacattgca tctccaattg catttaacat
    14641 aatatatgca ccatcagttt ctccatacaa acccaatgct gtaaatactg cattaaatcc
    14701 ttttatcata ccagcagcac acagaactcc taatattggt tgaaaaactc ctgatattat
    14761 gtctataaac ttattaagta atccttttgg cgcatctgag tctgaattat ctattgtatc
    14821 tcctcctaat ccagccaaat cgcacacaac ttcataaaca tctggaacat gattaccaat
    14881 aactacttgg tattgacctg cacttttcat tacagtaact ataccatcca ttttttttag
    14941 cacatcatca tttgctattg actcatcttt taatttaaaa cgtagcctcg ttatacagtg
    15001 agataaactg ctaatattat cctttcctcc tacattcgca atgatttctt ttgatagttg
    15061 ttcatacttt ctcatttttt ctcctccatt ttctttttga aatatgaagg tttggccaac
    15121 ttaatgtcac cacccaatat atataaacct aattattagg atttatatct gacttaatac
    15181 ttttacttac cactctttca acgtgaatag ttaagtataa tttttcctca tctgttaaat
    15241 cgtagttgta ttttttatgt ataaattcat ccaacttttc tatgaattta taagcatttt
    15301 tatatttaac ttttattaca tttaataaat catcttcatt attttcatgt aaattattac
    15361 taacaattcg ttctgcaaaa tactttaagt gagtaataaa tctatgataa taaactgaat
    15421 cttcatcaaa tactattctg taatggtatt taaccatatt tgaaatttct tgtattattt
    15481 gtgtaatctc atatatagtt tttatacttt gatccatttg tgcattaact atatgcagtg
    15541 ctataaatcc agcttcatct tctgatagtc taatttcaaa ttttttttct atataatcca
    15601 atgcctctaa ccctatttta aattcactct tataaaatct ttttatatcc caaagtaata
    15661 cattttttac acttactcct tcacgagctc tttctattgc agtatacata tggtctgtta
    15721 aagaaatata aatactatca tttaatcttt tccctagttt ttctttagca tataaaatta
    15781 tttcatctga taattccata tatcttatag gaatatctgc tattagttct ttaaatttat
    15841 cagatatatt tgggtctgaa agtttgaata tcttatcaat tttatccttt gatatatgct
    15901 ctcctgtacg ttttttataa gctaaacctc tccccataac tactatttcc ttatgatttt
    15961 catcagttgt aataattaca ttattattga gtattttttc aataatcatt gtgatgcccc
    16021 ctttattagc attaagttaa aataaatctt ttttaattat ttagaatatt taaaatgtaa
    16081 tttattacat aaaaaaaaac ctatttatta cacattaaat agactcccat gagtcctgag
    16141 taataaatag gtttagcttg tatttaaaca atcactatcc gtttgtattt tattttaatt
    16201 attatttatt atatgttagc aaatttattt tttagtgtca actttttctc aatattttaa
    16261 ttttaaataa attatataaa ttgaatcttc aaagtactat ttttatcaat aaacaaaata
    16321 aaaaaggtat tactttcttt aagtaatacc catttatttt taatttaagt ggtttttctt
    16381 aatctcatca agtagtccat cttctaaata aatttctaat gcacatccta ccattgcctt
    16441 tactgctttg tgaagtttat cataagcttc ctcagaattt gcataattta gaaattcttt
    16501 ttcatgaaca tataccattg gacttatatc taaggctatt tctgtataca tagtagggca
    16561 aacttgactt acattaccta tatctgtaga acctactgga ccatctttac cttctaatat
    16621 atctgttata cctgcctcaa tcaaattatt ctttgttatc ttttgaagta ctttgttatt
    16681 tattatgttg tcaaaagaat tttcaaagta tctataagaa agcttagctc ctgtcattaa
    16741 ctctgcacct tttgcacagt ttataacttt tttagttact tcatctaaat aacttctttc
    16801 agcagctctt acgaaaaact tacaagctgc tttttctgga actatatttg gagcctctcc
    16861 tccatttgaa actataccat gtattctaac atctgatttt acatgttgtc ttagtgcatt
    16921 tatcccagca aaagtaagtt gtactgcatc tagtgcattg actcccatat gagggtgact
    16981 agctgcatgt gctgataccc cttcaaaact aaactctaat gaatctatcg ccaaagcctt
    17041 acagtttata ttattaaaag cttctatatg catttgataa cttacatcaa catcatcaaa
    17101 agctcctgcc ttaactaaat cacattttcc tcctgttgtt tcttcagctg gtgttcctat
    17161 tagtacaatt tttcctttaa aattttcttt taacttactt aatactatac aagctcctag
    17221 tgtagatgct gcaatccagt tatgtccaca agcatgagct ggttctttat tttctccata
    17281 tccaggaagt gcatcatact cagctaaaaa tgctatggta ggtccatcat tatctcctaa
    17341 ttcagctcta aaagcagtct ccatattaca atatggataa actgtatcaa atccatattc
    17401 cttcattttt tctactaagt attttgatga tatgtattct tcttccccta attcaggatt
    17461 gttaaatata aaattacaaa cttcttcaat ttcttttcca agttcattct ctatctcata
    17521 agctctcttc tttatactct ctatcataat ttacctccta ctagaatcct agatataatc
    17581 ctattgtcaa aaatgccatt acaagaattg ttaatactac cattaaagga attacgaatc
    17641 taacccactt tgaccaagga atattggcta ctgctagtgc tcccattaaa acacctgatg
    17701 taggtgttat caagttagtt acacctatac caaattgata agctgtaact atcatttgtc
    17761 ttgatacatc tactaagtcg ccaagtggtg caagtacagg tattgtaagt gatgcaagcc
    17821 ctgatgctga tggtattaaa aatgctatta atccttctat taagaaagtt aaatttataa
    17881 atatagtttt tggtaatcca tttaataaat ttgctgcaga atttaaaatt gtatctatta
    17941 taaatccatt ttctgctatt attacgattc cacgtgcaaa tgctatacac aatcctgctg
    18001 taattaaatc tcctgctcca cttataaatg aattaactat ctcatcttgt ttaagccctg
    18061 atgctattcc tgatattata cctatagctg taaatatcat tgcaatttca gttatgtacc
    18121 attctagtct taaaactcca tatatcataa tcgccatacc tattgtgaaa atggctatta
    18181 ccatggcttc tctttttgta aattccttta tatttgtatc actattaacc attaattctt
    18241 gatttagtga tatatcatgt actaaagatt ttttagggtc ttttttaact ttattggcat
    18301 acatcataac aaatcctata gatatagcca tatatattat aaacataaca actctaaact
    18361 ctattccaga acctggtact atttgtgata atgcttgtgc tattcctaca gagaaaggat
    18421 tcgtagtagc tgctccaaat ccagctgttg ctcctataaa tactgtggcc actgctgtca
    18481 atgaatcata tcccatactg gtcataagtg gaataaatat catataaaaa ggtagtgtct
    18541 cttcactcat tccaaacgtc gtcccaccta gtccaaatag aatcattgaa atcggtatta
    18601 ttaaaaattg aaacttcttc attttattta cagtatgagc tataacagct tctatagctc
    18661 ctgttttatt aactatccca aaagctcctc caactattaa aacaaatcca actacttcag
    18721 ctgcatctat aaacccgttt ataggtgctg taaatacatc agtaattcct tgtggatttg
    18781 atggtgcttt ttcataagtt ccagctacaa caactgacct tccgtctata tctactcttt
    18841 caaaatttcc acttggcact atccaagtaa gaattgccat taacactatt agcaaaaata
    18901 ttattgtaag tgtctgtggc atagtaaact ttttcttttt taaactgctc atttttaatc
    18961 tccccacttt ttaaattaaa tattttatta ttgcatccct ttatcctatt ttgtatagat
    19021 gtaaaaaagc tcaacatgga tacatgttga gctaaatgtg ctaaatacaa catacctgca
    19081 tcttcccttt atcttgttta agatagctct ccatcaaaat tcgggataat tttgatgaca
    19141 gtactgtacc tattaaatac agtcccagca ctagcttcgg acactagcac ttcggcactt
    19201 actcctttta tattgcctca ttggtttcca acctcttcaa tattaatctt agtaaatgca
    19261 acctctacct caatctgttt gttagatgag gtaattctta ttaattttta ttaattcaaa
    19321 gtaattgtag cataatatgt cgagattgta aatattttca gtataataat tcttacttta
    19381 tgatttcatt ccatttttca caaataattt tttctccata taatttaaat actgtagtat
    19441 attttaaata cacacaaact ttccacaaat tttaaattat tataatacct ttataataaa
    19501 aaataaaagc tatgtaaact tatcaattta cacagcttta tatacattaa tctactccaa
    19561 gtacagcttt aagctttgat gctattgtaa taactcgtgg accaaatata acttgaactg
    19621 agtttttctt tttaacaact ccacttgcac ccaattcatt tacccagaag ccatcttcat
    19681 agaatccatc aattgaattt agctctaccc taagtctagt tgcacagttt gtaacatttt
    19741 ttatattatt acttcctcct aaaccttcta ttatagaatg tacaagcttt gtatctttat
    19801 catctacttc ctcttctact gcattgttgt atgctgtttt accagcatct acagattgtc
    19861 tttctctata ttctttctta ctataaagct taacttcttc tgaacttctt cctggtgttt
    19921 ttatatcaaa cttcttgatt gcccatgtaa atattatata agtaactata aaaaatattg
    19981 gtatcaaaaa taataatggt aacgcatcca ctttttgtgg ttgtaataaa ttaggaaaca
    20041 tatttctaaa tgattctccc attatagaaa cattaaacat ttctgtaaat acatacgtca
    20101 atcctgccag tggtacatat gctacaaaat acaataatgg tgcaacaaat aggaatgtat
    20161 actctattgg ttccgttacc cctaccaaca tggctgttac tattgctggt atcaatatac
    20221 ttattgcttt tttcttatta gcattatcag ccaacttata catagctaaa gctgctgctg
    20281 gtaatattgc aaaatgtatt aataaacgac ctgttgtgaa gttacgagtt atataagata
    20341 gtgtatctgg gtctcccatt tgagctaatc ttatattatt aacaccctcg taaattgctc
    20401 cacctacttc cattgttcca cctactcttg tatattctat agggaatgca attaagtgat
    20461 gtataccaaa tggtaataat gctttatcca ttgctccaaa agtaaatgtt cctaataatc
    20521 cactagaacc tatccaatta ccaagtttag ataatgccaa ccctactggt ggccatataa
    20581 agtacattgc tgtacccaat actattgatg ctataaacat cataatcatt gaaaatcttg
    20641 gacctgagaa aaaagataat gcactaggaa gttctatgtc aacaaatttg ttatgtactg
    20701 cactagctac aaatccagct attatagctc caaatatact acaatcatat gaaaagattc
    20761 ctaagaaaga accatataaa gaacttcgac tatatgcttc tgcctctcct aatccagatg
    20821 ccataaacgc ttctgctgta actgtatctg aatttatacc atttgctgct aacaatgtac
    20881 ttataactgt gtgcattcct ataaataaaa caattcctcc taatgcaccc catccttttt
    20941 cctttttaga taatccaaag gctatacctg taacaaagaa tattggtaag tatctcatta
    21001 ccataaatcc taaatcgttt attactttaa agaatacatt tataaatgac ccttctttta
    21061 ttagtccgct aagccctaat gcagaaacgt tttccatatt tgcaaaagct gaccctaatc
    21121 ctataaatac tcctgctaca acaagtaata taacaggtat catcattgct ccagaaagta
    21181 cttgtaattt atccttcata gtattatttc ctccattttt attgaaatgc caggctatat
    21241 aatgcatata tatatacttc tattgctttg tataatgtat ctatacttat attttcattt
    21301 gcctgatgtg aatttggatt atccccctct attgtgggtc caaatgcaac tccatttttt
    21361 aacactcttg cataagatgc agctcctttt gtaaaaagtt cggcttcttt tccaaaacct
    21421 tgtttatatg catttgataa tttatttatt aattcactat ctggttttac atacagtaat
    21481 tttaaatcct tatatatatc aactattaaa ttttcttttt ttccaatttc attaattcta
    21541 ttagttataa actcccaact tataccctta ggatatctaa agtctatttt catattcaat
    21601 tcatttttct ctaagaagat actcattata catattgtag aaactcccat atctacatct
    21661 tctttataca tccctaattt ttttccatga ttatcatctg taaagtatga atttaaaata
    21721 tcccttatta taacgccttt atcatttagt tttttaattt tctctaaatc ttttgctaaa
    21781 ctaaatgcac agttatatga cctgtgtgga tttctcgata aagccctttc tcctgtatat
    21841 cttactaata ctttaccttc atctaattct tctatctctg tataataagc taatgattct
    21901 actaaatctt tcttatcatt agttttaaaa acagcctcaa ttttgtcaca tacaaatcca
    21961 tcttctttgt ttgactttat atcaactaaa ttgtgatttc taaatttatc tttatctatt
    22021 ttctttctta gattgaagta taaaatacct ttttctccat ttactattgg gaagtttcca
    22081 tctggagaaa atgcaaattt tggctgttca ttgacactaa agtaatgctc catacattcc
    22141 caagtcgtct cttcagctcc acccactatc aatctaatct ttctcttcgg cttttcatta
    22201 agttctctta agaaaagtaa tgcatataaa atccctatca aaggtccttt gttgtcatta
    22261 actcctctac cataaagata gttatctttt tgacatactt taaaaggttt actcttccaa
    22321 agttctttat tatatattgg caccacatct atatgattaa gcacccctac tacttcttct
    22381 ccttctccat attctatatg aattgcatat ccatcaaagt cttttacaac aaaatctttg
    22441 tcttttgcta tttgaataag cttatcgaag gcatttctta tttctatccc aaatggttga
    22501 tttatatctg ttgtactttc atctctaatt gaaggtattt caactaaact tctaatatca
    22561 tttatgatat tttcttgatt ggattttaaa taatcaataa ttagttcttt gtttactatc
    22621 atcttaatta tttatcaccc ctttatatta attttacaaa gcatttcagt tcatatctac
    22681 attataaatt ttgaatatta ttttttcaat atttcaaata cttctctaag ttaccataat
    22741 gatacggtaa acaatcaaca gattatgaca aaatcatact atacctatta ctaatatttt
    22801 aacttatcta ttacgtcttt tttaatgtaa gtttattcca aaaatacata aaaaaagatg
    22861 tagccttagc tacatcttct tatatctatt caacttccat tattatgcta ttaatatcta
    22921 catttccagg ttctatccta tcttttactg ttctcttaac agaatcagca tcatccatta
    22981 taactacagg agttattata tttaaatcat ttgacgttat aaaatctaaa tctacttcca
    23041 ttattaaatc tttagctttt accttatctc ctgcctttac cttaacatca aacggctttc
    23101 cttctagtga cattgtctct aaacctacat gtattaatat atttgtatta tcattactct
    23161 taatcattat tgcatgcttt gtatcaaata cattgactat ttcaccatca acaggagaat
    23221 ataacttacc ttcacttggt attattgcta ctccatctcc catcatcttt tgggcaaaaa
    23281 ctgcatctgg aacatcttct aagttcacta aatttccctt aaaagggcta tatattttct
    23341 caatcttatt ttcttttttc ttaaatatat taaacataac acactctcca cattataaaa
    23401 ttttatgata atatttttct taaagctttt gctacaccat catttacatt agtatcagtt
    23461 acaaaatctg ccatagattt tacttcatct gtagcatttc ccatagcaat accaagacct
    23521 gcaaatttaa tcatggatat atcattttca ttatcaccta tacaaataac ttctttgcta
    23581 tctataccta atatatcaca aaatttttta actgcaatac cttttgaagt acctttactc
    23641 ataacttcaa agtttatctt tccagaactt actacttcta aatcttcttg ttttttaaat
    23701 tcttcctttg cttctctcaa agaatctacg ttttctgaga aaagtataca ttttaaaatt
    23761 tcattatcag ctttttctaa taaagtttca caacttgcat tttctattat ttcaattctg
    23821 tcttcttctt tatttttact atttattaat ctatatggat gttcttcacc tatttcattg
    23881 ttggatataa ctattttaca gcctttaaaa tgtttgttta aattatactt ttcacctatt
    23941 gtatagattt tctttagagc ttctttgctc aaaaccttct tatactcata tcccagtaat
    24001 ttaaaatatg tcccattagt tgatataata tatatatcat cccctaaaac acttgtaaaa
    24061 tatttcatag cattataagg tcttcccgta actaaggcta ccttgatacc cttttccatg
    24121 gcttctttta gtgctttaat attttcttct gatacttcaa atcctttacc catcaaagta
    24181 ccatccatat cagtacaaat taatttatat cgcatttgta tcacatccta gtaagatttt
    24241 tctaatattt caacaaggtc ttctttcttt ccaaaaggta aaaatcctaa aactggacag
    24301 cttgttattt gttctaattg ttcctttgtt acattaaact cagacatttt tgtcttaagc
    24361 cccactctct ttaaaaaact ttctatttca tttcctaaag ttttagctgc ttcatcatct
    24421 tccacttttt ctaattcttg gtttagtatt ctcgctactt tggcaaattt ctctatatta
    24481 tccttataga acttttctgc aaattttggg aacactatag ccaaagcctc accatgagga
    24541 atatgtgtaa ctcctcctat tatttcactt agtggatgtg gtgctgctgc tccagcattt
    24601 gccagtgaac ttcctcctaa tgtatctgct actgctaact ttgttctgta ttctatatta
    24661 tttttatctt ccattacttt aggtagatag ttaatgacta attccataga tttttcagac
    24721 attatctctg taaaatcatt agcatttgga tttatataac tttcaaatgc atgagtaaaa
    24781 gcatcaaatg ctgttgatgc agtaattctc tctggcagag ttaacataag ctctgggtca
    24841 actatacatt cttttgagaa acaattttga tgaaatattg tatttttctc gcttcctctc
    24901 gatattactg ctgcttgtgt aacttggctt cctgttccag aagtagttga tacagctatt
    24961 aatggtaatt ctttttcact taccttatcg tatattttaa atggatttgt atatgtacta
    25021 aacatataat cccaatctat tttttctaaa ccaaaagtca atgctaatac ttttgcagta
    25081 tctatacttg aacctcctcc aacagctaaa acaacgtcta cagattcttt tttagctaat
    25141 tcaaaccctt cgtttattaa ttctacagtt gggtttggct ctactctctg aacctcccat
    25201 gactaaagtc acagggttcc tgcttcacag aattttgcat actaaaatat gtcttactaa
    25261 ttctccacag gccatccccg tagttcctac ggttcttaca taagttattt attttgaatt
    25321 gttattagtc ttaatccttc ttttaatata tttatacttg catttatatc tctatcgtga
    25381 gtttcattac aactaggaca aatccattct cttatattta agtccttcac ttcctcgttt
    25441 ttatacccac atttattgca tatttgtgaa cttgcaaaga atttacctac ctttacaatt
    25501 tgtctaccat gccaattagc cttgtattct aattgacgaa taaattctga ccatgataca
    25561 tctgaaattg agcgagatag tttacgattt ttaatcatat tctttacttg taaatcttct
    25621 atgcaa
//
LOCUS       DAGSWI010000052        25460 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.052, whole genome shotgun sequence.
ACCESSION   DAGSWI010000052 DAGSWI010000000
VERSION     DAGSWI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25460
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.052"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(325..675)
                     /locus_tag="MJF80_001937"
     CDS             complement(325..675)
                     /locus_tag="MJF80_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBZ0280599.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(691..1107)
                     /locus_tag="MJF80_001938"
     CDS             complement(691..1107)
                     /locus_tag="MJF80_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBZ0280600.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(1126..1317)
                     /locus_tag="MJF80_001939"
     CDS             complement(1126..1317)
                     /locus_tag="MJF80_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBZ0280601.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(1468..2283)
                     /locus_tag="MJF80_001940"
     CDS             complement(1468..2283)
                     /locus_tag="MJF80_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBZ0280602.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(2330..3268)
                     /locus_tag="MJF80_001941"
     CDS             complement(2330..3268)
                     /locus_tag="MJF80_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBZ0280603.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVMISLFAFTPVRGTKMEKIS
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(3265..4137)
                     /locus_tag="MJF80_001942"
     CDS             complement(3265..4137)
                     /locus_tag="MJF80_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBZ0280604.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(4162..6090)
                     /locus_tag="MJF80_001943"
     CDS             complement(4162..6090)
                     /locus_tag="MJF80_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBZ0280605.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(6202..6579)
                     /gene="gcvH"
                     /locus_tag="MJF80_001944"
     CDS             complement(6202..6579)
                     /gene="gcvH"
                     /locus_tag="MJF80_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBZ0280606.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(6666..8792)
                     /gene="cdhC"
                     /locus_tag="MJF80_001945"
     CDS             complement(6666..8792)
                     /gene="cdhC"
                     /locus_tag="MJF80_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBZ0280607.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(8849..9655)
                     /locus_tag="MJF80_001946"
     CDS             complement(8849..9655)
                     /locus_tag="MJF80_001946"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBZ0280608.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(9705..11072)
                     /locus_tag="MJF80_001947"
     CDS             complement(9705..11072)
                     /locus_tag="MJF80_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBZ0280609.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(11103..12047)
                     /locus_tag="MJF80_001948"
     CDS             complement(11103..12047)
                     /locus_tag="MJF80_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBZ0280610.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(12067..12837)
                     /locus_tag="MJF80_001949"
     CDS             complement(12067..12837)
                     /locus_tag="MJF80_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBZ0280611.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(12893..14278)
                     /gene="lpdA"
                     /locus_tag="MJF80_001950"
     CDS             complement(12893..14278)
                     /gene="lpdA"
                     /locus_tag="MJF80_001950"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBZ0280612.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTIKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(14322..15203)
                     /locus_tag="MJF80_001951"
     CDS             complement(14322..15203)
                     /locus_tag="MJF80_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBZ0280613.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(15238..15912)
                     /locus_tag="MJF80_001952"
     CDS             complement(15238..15912)
                     /locus_tag="MJF80_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBZ0280614.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(15949..16821)
                     /locus_tag="MJF80_001953"
     CDS             complement(15949..16821)
                     /locus_tag="MJF80_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBZ0280615.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(16913..17545)
                     /locus_tag="MJF80_001954"
     CDS             complement(16913..17545)
                     /locus_tag="MJF80_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBZ0280616.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(17650..19326)
                     /locus_tag="MJF80_001955"
     CDS             complement(17650..19326)
                     /locus_tag="MJF80_001955"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBZ0280617.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(19575..20354)
                     /locus_tag="MJF80_001956"
     CDS             complement(19575..20354)
                     /locus_tag="MJF80_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBZ0280618.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(20664..22583)
                     /gene="cooS"
                     /locus_tag="MJF80_001957"
     CDS             complement(20664..22583)
                     /gene="cooS"
                     /locus_tag="MJF80_001957"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBZ0280619.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(23190..24101)
                     /locus_tag="MJF80_001958"
     CDS             complement(23190..24101)
                     /locus_tag="MJF80_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBZ0280620.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(24217..24795)
                     /locus_tag="MJF80_001959"
     CDS             complement(24217..24795)
                     /locus_tag="MJF80_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBZ0280621.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            24785..>25460
                     /gene="cdtR"
                     /locus_tag="MJF80_001960"
     CDS             24785..>25460
                     /gene="cdtR"
                     /locus_tag="MJF80_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HBZ0280622.1"
                     /translation="MIFILYILSICALIYLYILNNILIKEGFDNDVVRLYHNANLLLK
                     ELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGIF
                     DYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISFD
                     EVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLDL
                     KKNNIKM"
BASE COUNT         7474 a         5207 c         2939 g         9840 t
ORIGIN      
        1 atataattac ttaacataaa ataactcaaa ttgcttgatg aggcttttgc agtatttaaa
       61 gaatatataa ttatttaaca ttaaattatc caatcttgta ataaaattta ctcattaaag
      121 tatattacag aaaatacctc aaaataaact ttttagttca tgagatactc ttttttacat
      181 gaaattagat atatttttat catttaaaca atgaaaggga gcttatatcc atctttagac
      241 aaccccttat ataaaatttt taataattaa acatcacaaa atataaaaat cgcttatatc
      301 aaacgtataa gcgattaatt atatttacat atgtttttta gcctctaaca acattttatt
      361 agcttcttcc atatactcta tagccttttc aatactcttt gctgtttctt cttttccaat
      421 agctctagcc ttttcaaccc actctctaaa tccttcttca tgagtttcat tgtgatttat
      481 ccaatgtact aaaagtattt ttaaagtttt ttcatcctta gactctgatg attcctcatg
      541 actatgttcg tggtcgtggt cgtgagcatg gtcatgtgga tgttcatgac atatatctcc
      601 atgagtatgt tcgtggcagt ggtcatggtc atgatgtcca tgatgatatc ttacaatctc
      661 tttatcaaaa atcattagtt attccccctg ttattttata ttaaactctt tttttataag
      721 gtttacagaa tcttttacaa gtagtgctaa tggtttacct tctaatccaa ctaactccac
      781 attttctggc ataataggta aaagtatctt aagtgcctca ctgtctgcaa tagcttcact
      841 tatattacaa gtaacttctc ccatcataga attaggtatt attacagaaa taggtgcaac
      901 tattatattt gcttttttag ctgatactac tatagcattt tcccctgtag ctcctttatt
      961 agcatgagct ttcatcatag aactagttgc tatagaatta gtccctagtg cataaatttc
     1021 tacatatgta ggtaactctt ctctcaaaga agaaactatc tgggctccaa tacctccacc
     1081 cattccatct atcactgcta ttatcatatg aaattcccct attaattaat tttcttgtat
     1141 tattatctta tggtccatca atcttatttc tttaattatc ccatctatta tcttttgctc
     1201 tcctaataaa tccgttaaat aaatctttcc atcacaaggg tctatattaa ccacattttc
     1261 cataaccttt tctaactcat tattactttt acaaatgaaa gctgatgatt cacacatata
     1321 aatctcctcc taatatgatt tttattaaaa atcaaaaaaa gattccctta ggtatttcaa
     1381 tacaccaaag aaaccttcat gatttcagtt tttgtatatt attatacctt aagtatatca
     1441 ctttaattta tgtaaagtca attaaagtta acgcttttta ttatactctt cattcttgta
     1501 tttttcatat agttctttaa ctaattgctt ctcataatca gtcaaatcgc cctccacaaa
     1561 ctcaacatta aattgttcag ctaatccctt tactatgttt ttctgtagaa tatctatgtc
     1621 tatttttctg ccaatttcat tttctattcc acttgctttt ttagctgtaa atttcatagc
     1681 tctttctttc aattctggtg tcttagtttt tattaattta aacaattttt ctacatcaaa
     1741 atccagtatt attgaaccat gttgtaataa aacaccatct tttctgcttt gagcacttcc
     1801 aattaccttc ttattattaa tagtaacttc atatgatgca tgtgcattaa aacatgcagc
     1861 tgataaattt tccctactta ttctctcgcc tctatttaaa ttatctgttt ctataccact
     1921 tagattcaat cctttaacta gaccttcact tatatatcta taagataggt tgatgctctt
     1981 atcgattaaa ggattatctt ctcctataat tatactatag gttagctcat tatcatgtaa
     2041 tacagctctt ccacctgtaa ttcttctagt atagtctata ttcatacatc tacacttatc
     2101 taaatctatc tcatcttcca atttctgaaa atatcctata ctcaaacaag ctggttccca
     2161 tgtataaaat ctaagagttg gcttattatg tcctttctta tatgcagtaa atatagcttc
     2221 gtcaattgcc atattcattg ctccttcata tgatttatta tgtataactc tccactgatt
     2281 catgattatt acgcccccat ttttagtata aatatatcct agaaatcctc tatagattta
     2341 tttctcttat tgctaaatct atctcacttt gatttaaagg tcttgaatag ttgtatatag
     2401 tacttcctgg tctttcatta tagtatggtc tattacaatc cttacaacct cttatttcaa
     2461 aaggatagcc tttgtttatg tcttttaaaa tatcattgtc aagctttata ctatttaagt
     2521 atccattttt aaacttaaaa tactcttttg gataaccttt atttatcata taagacatta
     2581 actgtaccct tctataactt tctatgcttg gttgacttat cttttccatt ttagttcctc
     2641 taactggagt aaatgcaaat aaactaatca ttacatcatt ttcctttaaa taagtatata
     2701 aattgtatat atcttcatga ctttcaccca taccaactat tatatgtgta cttattttat
     2761 tgggatatga ttttgacatt tcagttataa attttaactt ttcatcatag ttatttcctt
     2821 ttattttttc atatagttct ttatttgctg catctattgc tattcctatt ttatcaactc
     2881 ctactgaaaa aaacttcttt atctgttcat cattttctaa ttttgcagat agtgaaattg
     2941 gcatgtctat cttaccactt atatagttta taaagtctaa aacagagtta tgtgcttctt
     3001 cacttgccat tgattgaata catattctct ttatattttt tcctttataa gcttttaaag
     3061 catctaatat ttcttctttt gaaaactctg gccaaattac tcttgataat ttatcttttc
     3121 tagtactact ttctattgat tgagaacaaa aactgcattt atttatgcat ttttctccaa
     3181 tcattatata tgctgtggta ggaggtatat cgcttttttt gtttaatatc ccaagttcta
     3241 ttgctgtccc aactgatagt ctaatcataa aacgcaacac tccttacact catttattgt
     3301 catattcatt tctatagcct tattctttgc agaccttgat ggtaatacta ttctattgac
     3361 tccacacatt atagataatt ggtctagctc ttttctatac actcctcttg gtctcataca
     3421 acctagatta atttcagtat ctggtaaatt aattctagct tcacataaaa tatctgctac
     3481 cccttctaac tcaggaggtt ctacattttc atactcagtt ccttttgttg gaataaggac
     3541 tataaatgta agcttattag gaggattttt ctgtaggtat tctataattt catattcacc
     3601 ctttatttgt cctcctttta aaccaataca tatatgtggt gctacttctg tatgttcttg
     3661 tattgtatta tagacttcaa tataatcttc tttactcttt ttcatcttat aaacttctct
     3721 tattgtctca tcatcaaaca ctaaatcaaa agaaactata tctaaatact tacacaactc
     3781 tacaatgcta tctttatcca taagacctaa atgagcattc aatctatatc cttgctcttt
     3841 taagttttta atggtattaa tatgagtatt tatagggaca tctccctcat aactacaacc
     3901 gccactcaat agaaagctag ttatattcct tgattgtact ttttcctcat attcttctat
     3961 tggcaccata ttgtttaagt agtgaccgtt gcaatgggca catttcaaac tacattgatt
     4021 gcttgtagta ctaagagcta gtgtctggtt aggataagca aactctattt tatttccaaa
     4081 attctttaat ttaatatcaa atgctatttt cattttttca gatagattct tattcatagt
     4141 ttattccttc ttaagtacat tttactcttc tagtaatttc tttgctgttg ggaactgctc
     4201 tatattcgta tgtggtaaga aacatgcaga aataaattca tccatataac ttggatatac
     4261 actaagttca acataagtta tttgattacc tatttcttca agtttatttt ttgcatcttt
     4321 acttataaga gctaagtaag aaccaacaag tgaactattc cctatataag taaatttttc
     4381 tctatcaata tcaggaagta accctataat tattgagttt tctatgtcta ggttgtttcc
     4441 tataccacct gctatataaa ccctatctaa tatactaaaa tccataccta aactatctac
     4501 aagagtgtat gcgcctgaat atatagctcc tttagctctt atgaaattat ctatatcaac
     4561 ttcattgact actatatcat tttctaaatc aaattcttct ttaaatgcta atacatactc
     4621 tcctatctca tgctcattaa atctaactct cttattatct aaatccctgt atattttacc
     4681 tcttctatct attacacctt tggtaatcat ttgacagatt aaatcaatta taccagaacc
     4741 acatatacca actggtgcac attcatcaat aatctttaat gttggctcta aagtatcttt
     4801 atcaataatt actttctcta ttgctcctgc tgatgctctc attccacagc ttatccctcc
     4861 accttcaaaa gcaggtcctg ctgaacatgc acagctcatc atataatctt tatttccaaa
     4921 tacaatctct ccattagttc ctaaatctat aaataaaaca ttttcttcac ttgaccatat
     4981 ccctgctgat aatactcctg ctgttatatc tcctcctaca taacttgcaa cagatggagc
     5041 taagtacaca tatgcactgt catttacaaa aagaccaaca ttttcaccca taagttttgg
     5101 agattttaaa aatggtggta tgtaaggttc ttgtcttaag aagtctgtat acacacccaa
     5161 gaataaactt gtcatagttg tattcccagc agccactaat gtaactacat cttccttatc
     5221 tattccattt ctttcatata ttgtttttaa tagtggattt atagtttcct caactatagc
     5281 tttatgaagt gtctctaatc catttttctt agttgaatat ataattctat ttataacatc
     5341 tgctccatat tttatctgtg catttccaga agatgcttta tctactacct cttttgagta
     5401 taggtctact aaacaaacaa ctactgaagt tgtaccaata tctattgcaa ctccgtataa
     5461 ggaattttct ttatttcctt gttctatatt tatgattgta agtttctttt gtttttgaac
     5521 atatgttatt gtaaccttaa aatcactctt tctaaataca gttggcattt ttctaagtat
     5581 atccaatcta aaatctattt cattatatcc taggttattt ctaacatatc tttccaatct
     5641 atctacatca cttatattat catctaaatt tggttcttcc atttctatgt atttcttttt
     5701 tatatttgtt ttaaattgaa ggttatgttc ttcaattatt tttttagctc tttcaaaaat
     5761 ttccctatcc tcttttttat tactaccttc tatcttcata ccatgcatag aagatgatac
     5821 tttagaagga acttcaattt ctatatctga tattaccttt gtacaacaag ctagtatata
     5881 accttgctcc cactcatcat ctgttatatg tcttgttttt tctgtatcaa cttttccatt
     5941 caataatttt actttacact tcccacaaga cacgtttcca ttacaaggag catctatgaa
     6001 tatatctgca tttcttgcaa cctctagtaa tatatccccc tcattacaat aaacctcttt
     6061 attgtttggt gtaaaactta cttttatcat gttatccctc ccaatttgtt ctatatatga
     6121 taaaaggaaa gtatagctaa atttgcgtgc actcaaattt tactatactt tccccaatta
     6181 taataatatc ttaatcttta attattctaa tccagcctcg tattctttat cacttaataa
     6241 cgtatcaagt tcagccacat cagctagttt tacttttaat atccagtttt catatgggtc
     6301 ttcatttata cattctggct catcttctaa tttttcatta acttccaata cctcaccatt
     6361 aacaggagat attaaatcag aagctacttt agaagactca actactccaa aatctactcc
     6421 cttagttaca gtatcttcta cttctggtgt ttcaacaaac aatatttctc ctaattgatc
     6481 ttgagcatag tctgttatac ctatatatac aacatctcca tcaattactt ttacccactc
     6541 atgatcttta gagtatttta attctggtaa taatttcatt tcgatagacc cccaatatta
     6601 tattattgat taaattactt aaatcatatt aatattatat catattattt taaattttcc
     6661 atcaattaca ttacactttc catagctaat gctggatgac ctttttcttc taagaaagct
     6721 aatactcctt cagaatcgtc agctatagtt tcatcacaaa ccatatcaca gaagttttct
     6781 attccagtca tttctttaac tgtagcattt aatttatcag ctacataatc ttttaattct
     6841 tttggcatcc aaactattct ttctggtcca ccttctgcat aagcgaattt tttagaagat
     6901 atgaaatgtc ttccgtgtcc cataaatcct ggagtttgaa ctcctccacc tgtcatagaa
     6961 gcaagttctc cgaaagtcat accaactgga gtaacacttg caaattctct gttaactaca
     7021 acaaatccat ttgcttctgg cattatacca cagatacatt cgaagcatcc acaagaagtc
     7081 attgggtctt ctagtataga gtataatgta acagattcaa ctgcaccttg agatatttgg
     7141 tttacagttt catttactga attccataca cctgttctat catctaagca ctcgcctttt
     7201 tctattggtt gacaaggacc tgttgggtct aattctttag tagccttagc atctaaccag
     7261 ctaactgctc cacaaagacc aagtctttca ggagtaacaa cacaaacgtg agctggtgca
     7321 aatgattgac ataagttaca agaatagaaa gtgtcaacac tttcatcaac taatgaagct
     7381 aatctttcat ctctagcatt gtacttagct attgcttctc cttttaattc agaaactttt
     7441 tcagcatctg taattatagt tatttcacac ttatcaacta ctgattcaaa ttcatctaac
     7501 atcttagcat ataaaacttc tcctatatgc tcaagtctaa atcctttatc aaatgcatct
     7561 ttagagattc ttacccaagc catatctctt tgtccaacgt gcattacacc ttctatatag
     7621 tttaagaagt agtggaaacg tctttcaaga actggctcga aatcttcttg catattttta
     7681 ccagctattt taactattac agcaagtggt aatcttaata caccatctgc atcaacttca
     7741 tctatatttg gtcctattat ttctatctta tggtcttcaa cttctgttgc ttctttctta
     7801 acaacaagct cccaagcttc agttctgttt ccaccaaact cagcaaacat atcgctcttt
     7861 ctaattctct caccttcaaa tgctgctgca aatgaaactg ggataggtat ttcagtaact
     7921 tttattttta ttcctctagc ttctaatgaa gtagctacta tcttatcgta atctttttga
     7981 gttaataggt tcattggaac ttcaagaact gtttggtcag ttataactgg gaatcctaaa
     8041 gctattgctc cagcacctgc agatacaact aattcactta atggtccaaa tgcattaaca
     8101 aatgcaggaa ctctattagc tgtgtactct aataatccgt ttaaatcacc aggagttaat
     8161 ccaccgaata ttaatgctgc tcttactgca acagatacaa cgtgtataac agaagttaca
     8221 tcatatccta gaggtataac tcttagctca agacccattt ttactccagc ttctatagct
     8281 tggtctataa ctttaccaac taagaaagtt agtaaacctt tagattggta atcttttata
     8341 acttttgctg ctgattctgc atctggacac tcaccaagaa caactgcaac accaggtata
     8401 tctcctgtaa ctaatggtac accaagtgat ctgattatag ggtcagttat atgacctgca
     8461 catggctcac tatatggagc atccatagtt gaatatttta atgcttcaat tacttctgct
     8521 gctaaagctg tagctaaacc agcattaaaa gcatgttcta gtaaatgagt tctatttatt
     8581 aaagatttta ctacttctaa agcaccttct aagtccccta aagtgttcat cttttgtcct
     8641 gttgcagcat atatacaagg taatgaatat gctgtatcag ggaaagcaac tttatgctct
     8701 tttccattct tttctatagc ttcagctaac atagcttgag ccgcacctaa agcttgttct
     8761 gaccctgtaa agattatatt atatagattc attctatttc cccctttaat atttacacct
     8821 attttttatt aactataccc taaaaaccct agaaagcaaa tccaccttca tttaactcta
     8881 agaaagagtc tgcatataaa gatgccccgt tgattatgtc acaagatttt acagttttca
     8941 ttaattctgg gtcacatgga ttcattatag ctgaactaaa tccattagcc attgccattg
     9001 ctaagaatgc actatctaaa acaggtctaa catgcttagg acatccatta gatacgttag
     9061 ataatcctcc tgtagttaaa agtcccatct cagtcatcat ttttatagct tctaaaactt
     9121 ctacttgttt ttcttgcata ccttttataa ctaagcataa tgggtcaaat agtatatcag
     9181 ttggctccat tcctaacatt aagccttttt ctaatatttc ttggcagtaa gccatacgct
     9241 catcattatc tcttgggata ccttcttttg cacataaacc tataaccatt gcttcgtatt
     9301 cagctgctat atctattaaa tcaaatcttg agccagcatc agcagagttt attattggtt
     9361 ttgcattagt tctatcataa actttaagtc cagcttcgat agctttttta ttagctgtat
     9421 ctaatgctat aggaacattg ttgaactcag attgaagtaa tttgattccc catgtcatta
     9481 tttcttcgcc atctctttcg gcaggtccta tattaaaatc tatatagtgt gctcctgctt
     9541 ctaattgctc ttttgctctt tttaagattg gagctgggtc tctttctgct aatgcttttc
     9601 ttattgaagg tgatatacaa tgtattctct cacctataat cataaatttt tccatttata
     9661 atgtccccct ttaaatttaa tattaatagt tagttaatat tttattagtt tgtttggcat
     9721 gctttttctt ggtatccttt taagaactta ggtaattcca tagattcctc tggtcctata
     9781 actacattcc atcctggtaa gttatcttct atatcacctt taagtactgc aacttttcct
     9841 ggaatgataa ggtctttaca attagttact tcttctacct tactttcttt aatgaaagca
     9901 gagattgagt ttccaccaaa tttaccagca gcccaagatg taagaactga atatccacct
     9961 gcatctggta ttactaacca tactggaact tttgatcttt ctatatcacc agcaactatg
    10021 aagtaagtta atgcaaagtc aacagtaact aatactggag aattttcatc tggattgttg
    10081 attggataaa tctttggttc aactctcatt ggtctttgtg ggtcagtgta tatgttttgt
    10141 cttagtgcaa ataatggtaa tgccttagca taactaatat catctattac tattatagaa
    10201 ccgtatttta tagtgaatat tgatgataaa gcaacttcca tcatagggtt tgaattagat
    10261 aatctatttg caaatactat tgaaggatat ccaaatgttc tatcttgctc ttttaaagct
    10321 gttcttctaa cttgtatagc attagtgtaa gtatctttta tgttttctcc tgttacatct
    10381 aatactaatt ctttatatcc agcggcttga attaattcaa ctgtttcata taattcttct
    10441 aagctaccag cttttacacc taatggtaaa cttgctcctt taactacttc tatcatatct
    10501 ttgtagttct ctttagttgc tccatatacc attggtctag catcttttaa tacttcaaca
    10561 gcttctttaa ctacttgtgc atcatcacaa gctaaaatat aagctacttc taatccactg
    10621 ccttttattt tatctattaa ttttaaatac gcatctttat ctccacaata ctctaaaaca
    10681 gccatttcaa ctttcatttg ttcaccaatt cttacatagt caactttttt catgttagct
    10741 attttagcat ctactgcttc atcactcata caagtacaga atgaaactgc atatctattt
    10801 ctacttacta atgttttttc atgtctaaat aatactgttt ctccacctag ttcatactca
    10861 gatgcaccag caccaacttt taaagctttc attaaaggtg cagtagcctc tgataatttt
    10921 gctatagcgt catcagacat atgtgggcat ttacctactt ctacagctcc tgaagcaacc
    10981 ttcatagaga aagccataca agttgggaat ccacaatcct tacagttttt ctttggtgtt
    11041 aatttaaata tatctaaagc ttttaatgcc attatttttt ccccctaacc ttaacaattt
    11101 aattaagcta atgcgttaac caattctttt atagtttcta ttgattttgg atgacgaagt
    11161 ataactgcgt ttgaaccacc tactaaaaca cttgctgcag ttgaaacttc cattgctata
    11221 gttctttctt ctggacaacc ccaatctggc tcatcttcta ttggagctat agcttcttta
    11281 acatgaccta cttcaaaagc tactggtgtt atgataggca tttgtaatgt cttatcattt
    11341 tgaccaaatg ctgcaagtct aattctatcc atagtagatg caacgtactc atatccataa
    11401 ccaactgctg aacatccaac attcataact atattttcac ctttaacgcc tagttgagtt
    11461 aacaatacgt ttaattgttt agctaggttt atatcaacag aagattcagc tcctacttta
    11521 tgtgcataag ccataccagc tgatgctcct actcctttgt agttatcttc tactgctgac
    11581 ataaataagc agttatgtcc atccaatgtt tgagctaatt tttcaaataa tttaccatct
    11641 ttttcatgat ttcctgaacc agctacaact aaaggtaatt ttattgcttc aattactgcc
    11701 ttagctacat cagcacattc atcaactgac ttatctaatc catttgggtc agaaccatca
    11761 aattttaaac aaataaaatc tgcttgtgta ttagcttcaa catatttagc ccactctact
    11821 ggacaattag ctacatcttt gtataattcc ttatatgaat cagtccagct ttcaggataa
    11881 acgtctgata tttgtatacc tatttttgga gaattaccta cttctccatc aaaactataa
    11941 aaaggtaata cattttcccc acctaattta attgcttttt cccctattcc tacttcaact
    12001 tctgatattt ttccagaata tttttgagta gacattttaa atgccatatt tttttccccc
    12061 ttaagcctat tttaattcta atttagcaaa aatttctttc atagccactt tagattttga
    12121 atcttcaggt aaattaacta aaggaatacc tgtagaatca tattcatata tcaattcatc
    12181 cataggtaca acaccgatta gctctaggtt atgcttttca atttcttctt taacaccatc
    12241 attcataacc ccatttggaa ctttatttac aatcaaaagt attcttccaa cacttaactt
    12301 tagttcctca gctaaatctc ttattctagc aacagcttgt atgctacgtc ttgagcaatc
    12361 actaaccaat aaaagtgtat caacatgtct agtgactttt ctacttagat gttccatacc
    12421 agcttcgttg tccatgacta agtatttgta atgaccagat attttgttta cttgttctct
    12481 aagtatccca tttacaaaac agtaacatcc ttcaccttct gacctaccca ttactaataa
    12541 gtcatatcct tcgccttctt cgattataga atttaatcta aattgaagat attgtgcctt
    12601 tgtcatacca ccagggaatg cattgcctaa cttttctctt tgatttactt cttctcttat
    12661 ttctcctatt gttgcttcaa cttcgatacc taacacttca tttatgttag cattggcatc
    12721 agcatctaca actaatactg gtcccttttt gtccttaacc aaataatcaa tcaaaagccc
    12781 tgtgagactt gttttaccag ttccaccttt tcctgcaaca gctatattgt atcccatttt
    12841 aagacttccc ccttaaatct agccacagag ccttatgtac tctgtctgtc tattataatt
    12901 taggtacaga atgaacacat tctccatgta catcatgaag tgcttccatt agcccttcag
    12961 ataaacttgg atgtggatgt attgcatccc ctacttgttc tactgttaat cctagatgaa
    13021 cagcaagtga caactctgtc aataaatctg tagcatgagg tcctactact gctgcaccta
    13081 ttatcttatc agtttctttg tcagctataa cttttacaaa tccttggaaa tgtcctatag
    13141 cttgtgcttt tccaagacct ctaaaatcaa attgacctac attgtattct acaccttcag
    13201 cttcaagttg cttttcagtc ttaccaacac cagcaacttc aggttcagta taaacacatc
    13261 ttggaattgc tctataatct actaccttag tcttacctaa agcattttca actgctacga
    13321 taccttcttt agaagcaaca tgtgctagga aaggagtatc tattatatcg cctattgcgt
    13381 atataccttc tacattagtt tcaagatgtt cattaactac tacttttcct ctttccattt
    13441 caattcctat atcttctact ccagaattgt caaggttagg tcttctacca acacatacta
    13501 atgcatattg tgcttctata acctttccat tagaaagagt tgcaacagcc ttaccatcaa
    13561 ctacttcaca agtttgtact ccaatacctg taataacctt aattttatct ttcttaaatt
    13621 gtctaagtag ttgtttagct acatctttgt cttcatttaa tagtatttgg tcaaccatct
    13681 caactatagt tacttctgtt cctaaggctc taaagaactg ccctatctca catcctataa
    13741 ctccaccacc tactattaac atagactcag gtatctcttc aagtccaaga acttcatcac
    13801 tagttattac aacttttcca tcatatggga acattcttgg tacaactgga acagaaccat
    13861 ttgctagtat tatcttatct gcttttatag tttctactgt tccatcatct ttagttactt
    13921 ctatagtgtt tttatcaatt aattttccaa aaccattaac taagtttact cctctttttt
    13981 caaataagaa ctctatacca cttattaatt ggtttacaac tttatttttt ctttccatta
    14041 tggcagtgaa gtttggctta attgtaccat ctatttctat accaaaatcc tttgcttctt
    14101 ttacagtatt caatactcct gaagaagcaa gaagtgcttt agttggtata catcctgcat
    14161 ttaagcaagt tcctccaact cttctttttt caacaactgt tacatctgca ccaagcatag
    14221 aagcttttat agctgctaca tatcctcctg gtccaccacc tactactact atcttcatct
    14281 ttaattcccc caaacctaat ataatctaca atcttgcaat actataaacc tgcggcatcc
    14341 agtatagcag gtatattctc ttctgcccca attgccatta tatgaacacc atcacataaa
    14401 tcttcttctt ttaattgttt gataagttcc cctgccattt ttattccttc acttacccag
    14461 ttttcttttc cagctgctct taatctttcg atttgttcat ctggaacaaa tattcctggt
    14521 acatttgcag tcataaattt agccattcct ggtgatttta aaggtactat tcctgctaat
    14581 accttgcaat ccatatccct tgttaattct ctaaactttc tcattgtatt tatgtcataa
    14641 agtgcttgtg tttggaaaaa tttagcccct gcttttattt tctttttcat ttttaataat
    14701 tgaacttcta ttggagaata ttctggagtt actgatgctc ctagataaaa atctggagaa
    14761 ccttttaact tattaccagc catgtccgta cctgccatta aagtctcagc agtttgaagt
    14821 atccccacag aatctaaatc aaacactccc ttagcttgtg gatggtctcc tacgctagta
    14881 tggtctcctg ttagtgccaa cagattgttt atcccaaata cacctgctga caacatttct
    14941 ccttgtatag ctattctatt tctatctctc cctgttattt gaataactgg ctctaatcca
    15001 gcatctttta ataatttaca tgtagcaaga gaagttgctc tcataactgc cgattgaaag
    15061 tctgttacat ttgctgcatg aactcttcca actaacggct ttgcacattc tattaaatgt
    15121 gaaaggtcag tcccttttgg aggagccatt tcagtagtaa ctgcaaattt tccactttct
    15181 aatgtttctc ttaataagct catctctaat ccccctttta accttctaaa aaaactttta
    15241 tgaatttgct gctgcttctt tctttttctt tgcacttaaa cttcttggat tattttgctt
    15301 agaatagtct tttggtggtc ttatttctgc taagttatct aattgtccta tagcttctag
    15361 tctttcatat atctttatcc aagcacaatc attctctgag tttacttcac acttaccatt
    15421 tttagctcca ccacaagccc cattcataag acctttagca cacatagtta ctggacatat
    15481 cccacctgtc caaccaagtt cacagtctcc acaagcctta caggcttctt catattctcc
    15541 aactctttgt acttccccta taaacaaagt attgttggct ggataaactg gcttatcttt
    15601 taaattctta actattgtct gtgtaccatc tccacatgct aaagataaaa ctgcatcagc
    15661 ttccttagtc tcttctttta gagcctttaa atccttttta gactttaaaa ggttacaagc
    15721 agggtcaagc attacatatc ctaagatttt tttgccttca ccttcaagag tttccttcat
    15781 ttttaaaact tcttcttccc caccactttt acaagttgct gcacattgat tacaaccaac
    15841 taaaaccagc ttgtcaaaat cttttaagta ccctagcact tcttctaatg gtttattttc
    15901 agaaattatc atatctactt cccccctaaa ccttccacat atatatatct atttattgtt
    15961 ttgtaatttg caagccttaa ctacatgttt tgcaagtata gatgttgtaa ctgaaccaac
    16021 ccccgctgga actggagtta tcattgatac tttgtctaaa actgcatttg tatcaacatc
    16081 tccacataaa tttccttctt catcaacatt tataccaacg tctataacta ccgctccatc
    16141 tttaacaaaa ctttcatcaa ccatcttagc tctaccaata gctgctatta gcacgtctgc
    16201 ctctgctgct acacctgata gatttttagt ttttgaatga catattgtaa cagttgcgtg
    16261 ttcacttaat aaaagcattg atactggctt tccaactacc attgatcttc caagaactgt
    16321 taccttactt ccttttaaat caacattata gtgttttaat atctcaacta ctgctgtagg
    16381 tgtacatggt ggaaatccac ttttatcccc ttccattacc ttagcagagt ttattggact
    16441 aaaacaatct acgtcttttt ccggtgcaat aacatatttt ataacttctt catttaagtg
    16501 ttttggaaga ggtctaaaac ataatatacc attaacattt ttgtcatcat taactctctt
    16561 taatacatca atatattcct cttgtgttat atcctcagct aactctaaca cttctgttgt
    16621 tatacctatg ttttgacatc tctttagtgc tcctctttca taagataaat catcacttct
    16681 tgccccaact ctaactattg ttagtttagg atttatacct tctttaatta gtaaatcgac
    16741 ttcttttatt aattcttcac taatcttatc agctacaggt tttcctttta taatttgtcc
    16801 tttagttgac attccttcca tacattcaca actcccctac aagcaaactt aaattaattt
    16861 attctatata ccacatatta ataacatagt atctactttc gtcatttaaa aattattttc
    16921 caagagcttt ttcaactttg gcataaactt catcacaaat ttttacacca tcttcaacta
    16981 acttatctac ttctgttttt accttattaa catattcttt atcttgtata gaatttatat
    17041 ttataactac atttaattgt ccaccaagta tagcagctct taaacactga actcctacac
    17101 caacatcact tatagcaagt cttgaacctt tatcaactaa atcttcatgt aacttaatag
    17161 cttcatagca aactcttact atgtttaatg gtacttcaca agcaactttt aatgcctttt
    17221 ccattgtttc tgcttttatt cttttttctt cttctgtttc tttaggaagc ccgtaagctt
    17281 ttgataatgg taagaaacat tctgcatcat catctatcat ttttaataaa tctttttcta
    17341 actttcctgc cttacttaat atctctttta cactttcttc atactcagca tacttcttct
    17401 tccctattgt aaggttacat accatactac ctaaagccat acctattgca cctactagtg
    17461 cagcagctcc cccaccacct ggtactgctt ctttggaaga tagtacttct acaaaatcaa
    17521 cacaagtttt atctgctatt ttcatgataa tcctccttga aaggatgtag gaaaatatct
    17581 caaattgttc gagatatttt acgaactatt ttaagatatc tcctactctt tatttataaa
    17641 ttaatttcct tagaataatc cagctattac tccattttca tcaacatcta ttttttctgc
    17701 tgctggaact tttggtaatc ctggcatctt cattatttct ccagtaagtg caactataaa
    17761 tccagctcct gctgaaattg ttgcttgtct tacagttatt ctaaatcctg ttggtctacc
    17821 taatttagtt tggtcatcag ttaaagaata ttgagtctta gccatacata ctggaacttt
    17881 agtaaatcca agtttttcta agttagctat ttccttatca gcttcaggag tataatctac
    17941 accatctgct ccataaatct tagtagctat agcatttatc ttgtctttta tagataaatc
    18001 atcttcatag cagaatctaa agttattttc ttcttcatcc aataatctta aaacttcttt
    18061 agctactgca attccacctt ctccaccttt tgcccatact tcagaaagag caacattaac
    18121 acctaattcc ttacacttgt cttcaacaag tttaagttca gcttcagtat caagtgggaa
    18181 tctgttgatt gcaacaactg ctggtaatcc atatacttga gttatgtttt caacatgttt
    18241 taataagttt ggaagtcctt tttcaagagc ttctaggttt tcgttattta agtctgcttt
    18301 agcaactcca ccattgtatt ttaatgctct tacagtagca actattataa cagcatctgg
    18361 ttttaagttt gccattctac attttatatc taagaatttt tctgctccaa ggtcagcacc
    18421 gaaaccagcc tcagttatta cataatctgc aaagtgcata gccattcttg tagctataac
    18481 agagttacat ccatgagcta tatttgcgaa tggtccaccg tgtacaaatg atggtgttcc
    18541 ttcaagagtt tgaactaggt ttggttttaa agcatctttt aataatgcag ccatagcacc
    18601 attagctttt aattgtctag ctgttacagg ttcaccttcg tagctgtatc caactattat
    18661 gtttcctaat ctttctttta aatcagatat atcacttgcc aaacagaacg ctgccattat
    18721 ttcagaagca acagttatat cgaatccatc ttgtctaact gctccatcac cttttttacc
    18781 cataccatca acgatatttc taagttgtct atcgttcatg tctacacatc ttctccaagt
    18841 tattttctta gggtctattc taagtgcatt tccttggtga atatggttat caagcatagc
    18901 agctaaaaga ttgttagcag ctcctatagc gtgaaaatct cctgtgaagt gtaagtttat
    18961 atcttccata ggaacaactt gtgcatatcc tccaccagct gctccacctt taacaccaaa
    19021 tactggtcct agagatggtt ctcttaaagc aactaataca tttttgtcta gtttagcaaa
    19081 tgcatcagca acacctatag tagtagtagt ttttccttca cctgctggag ttgggtttat
    19141 agctgtagtt agtattaact tagctttttt accagtctct ctttttagta gattgtagtc
    19201 aacttttgct ttgtatttac cataaagttc tacatcatct tcacctagtc ctaatttttt
    19261 tgcaacctct ctaatatctt gaggcttagc ttcttgagct atttcaatgt cagatttaaa
    19321 tcccataact gaatctcctc ccacaaccta aatagattta aaatataatt tttgtaaaaa
    19381 ttgtattaat atgtaaaaat tatattttat aagataatta tacatgtaaa attacttaaa
    19441 aactagctac taagctatta aaatattcaa acttaatagc tagttattta tctaattact
    19501 tacttattac ctatctattg cctatgtcta acctttaacc aacttaaaat cccaaatcaa
    19561 aatttctatc tattttattt atccttaagt gacattaatt tatcttttat tgctttaatc
    19621 tgttctttcg ttgtttcact agaatcatat ggagattgat tagctctatc agaatcaata
    19681 acatcttggt tataataaat aaatcctaaa gattctccat cttctaagtt ttggattata
    19741 aattcttcat cttccttatt tctaatttta tttcctacta caaatacttt atttacacct
    19801 atatcatgtc caagtttctt aacttttcta taagtctgta gacttctctc tcctggttct
    19861 acaactacta taaaagcatc tacaccttgg gctgttcctc ttcctaagtg ttcaattccc
    19921 gcttccatat ccataaccac tacatcttta ttttgtagta ttaaatgtga gcagagtctc
    19981 ttcaaaagaa catgctctgg acatacacat ccagtaccac cagaatctac ggttcccaat
    20041 gtaagtaatc ttactccatt gtattcttta cagaaatttt cagggatatc gtctacttta
    20101 ggattcatct taaacatttt tccaaatgaa cctacagaag cagctgttct atctgaaact
    20161 aattttttca tttctgatat aggtactatt gattcatata cttcttttgg aaaacctagt
    20221 gctaaagcta aattagcatc agggtcggca tcaacagcaa caactctgta tccgtcttca
    20281 gcaaacatcc tagagagcat tgaagaaaaa gtcgttttac ctactccacc tttacctgtt
    20341 attgctatct tcataatccc taccaccttt tattactatt tgctttttag tagttaattt
    20401 tacagttaaa tattttttat actctatatt tttatacact aaaaataaac tacagctagt
    20461 ccttacagga accaattaaa tttaccctac tagcttttca aagcagcatg tatagtttta
    20521 ttattcttta atattgttta tattttattt attcagtata ttcattatat tttgatgttc
    20581 tttactttaa tatttgactt tattcaactt tattcaacca gaaacttctt ataaaaacgt
    20641 ttattctata acttcactat tttttaagct tcagcctttt ctgccatctt ttcttcaact
    20701 aatgcttcaa agtgaacacg ttttttctct atatcttcta ttattgtagc tgctaactct
    20761 tttggatttg tatttacagt aaatgtagct ccaactttat cttttaattc actagttaat
    20821 atatccactg ctcttgaaga accagctatt ggaggcatta ttcctaaata agtgtctata
    20881 ccagaagcta caacatagca tcctatagca actgcttttt ctgacatcca ctctggagct
    20941 atacctacaa ctggaaggtc acacatatcc atatctaagt aattagctgc tgctccaact
    21001 aaatctagta tacgacttat atcaacacaa gaacccatat gtagtactgg tgggattcct
    21061 actaatttac agacagttgc aagaccttta cctgcatatt tttcagccgc atcagattcc
    21121 attaaaccaa acttagctgc tgctgaagct ccacaacctg tagtaactac tattatgtcg
    21181 tttttgatta attctttcat tattgttacg tgagcctcat ttgaaactcc tttagcattg
    21241 ttacatccaa ctacaccagc tgctcctctt aatactccag attttaaaca gtctgttaat
    21301 ggcttaactg ttcctgctgg gtctatatga gagtttacaa ctctgtctaa ttgatttact
    21361 actgcttcta cactataccc aactgttgcg cctgatttta attcaggtat aagtactttt
    21421 gatttatctc tatttttaaa gtttaaaata gcttctttaa ctattgcttt agcatcatca
    21481 agtgcttgtt cttctctaaa ttcaatataa gttgaacctg ttatcttagc ttttggagaa
    21541 gttgttacaa atttagtatg gtaacaatct gcaactctag ctaatgcagg gaatatacat
    21601 tgaacgtcaa ctattacagc ttcaactgct ccagttatta ttgcaagttc ttgttgatgg
    21661 aagtctcctg ctatttttac accatgtctc attgtaatct cattacctgt acaacacata
    21721 cctgctaagt ttattccatc agcaccaact tctttagcta aagctactaa gtctggctct
    21781 tctgatgcta aaactatcat ttctgataat gctggctcat gtccatgtaa tattatgtta
    21841 actttatttt cttctaatac tgctaaattc gcctctgttc ttcttggcac tggagttcca
    21901 aataagatat cgcttagacg agtacctatc atagaaccac cccaaccatc agccatagaa
    21961 gttctaagac tcatgtgtat taaactatct atatcaccag tacatcctat atgagtagag
    22021 tgcattattg ttgcaatttc tctatctata gctcttggct ctatagcgta ttctttccat
    22081 attttttgtc ttggttctgg tgcatttttt ataaatctta atgtcccaaa aggttttcca
    22141 aattccatta atgcaatttc tgccacttca tgtgctacat catagatatc tctaccttct
    22201 gtctctacat cccattcttt agctaaagta attaacttag cttcgtctct tattttatag
    22261 tttccatttg gatcagctaa tgctaatgta tgtgctatat ctctagcatg gtctgaatgg
    22321 gcagctgtcc caccagcaac cattcttgca aagtttctac ctactatagt atgctcatct
    22381 gctccacata acccccttgg agtcttaggg cttattctac atggtcccat tccacatatt
    22441 ctacagcaaa caccttgaag accaaaacca cattgtgcct taaagccttc ttttctatca
    22501 tacattgttt ctacgccttc ttctctagct ttagctatca tcttctggct gtttaaatca
    22561 attgttaaca ttttttcatc catattccta tccccttttt ttgattttta aaatccccca
    22621 ggtgactgac ttcatgtcat aaaataataa aataaatgct ttctaagttg attatatact
    22681 aattttcagt gctttgcttt agatttaaat atttttttaa caaaattaga taatacataa
    22741 aaattttatt ttctacactt aatttaagtt gattttatcg cttgtttttg gctatttgtt
    22801 aatatctaaa cctacttttt tgatacttca gtttaattaa attatgtttt gattttttta
    22861 aatcaaattt gaaatttttc tagtcatttt tatattattg cagattgttt gtgtatttat
    22921 tatctttcac tactgtttgg acttattaca gatttttact ccctaaactt aattagtaaa
    22981 aacttcttag aatagcattt tttggactaa tttttttgaa tttttttatt tttcctactt
    23041 taatattaat tatagcttca aattgaacat ttgtatatat tcatttgaag ctataattat
    23101 tttttatata aaactttttt aatacagcat taatttagac attttgacta ttttttctac
    23161 tttaattcat ttatttcaag cgatttatcc tatttcatat ttttagctac aatagcccca
    23221 tatgcaatag aatataattt agattcatgt gaatcagctc ttgacactaa aactatagga
    23281 gcttttgcac ccataattat acctgctgat ttagagtctg ccatatatgt aaaggctttc
    23341 cctattccat taccaacctc tattgttgga accaatatga tatctacatc tccagatact
    23401 tcacttttaa acccttttat ctcactagct tcttttgaaa cagctaaatc aaaagccaga
    23461 ggaccttcta caattacatt ttcaccaaat tctccttctt tacaagcttt tgctaacata
    23521 tctgcatcta cagttgcttg catcttagga tttactttct ctttagctgc caaacatgca
    23581 acttttacag tttccattcc taaagctttt gcagctttta tagaattttt taatatcttt
    23641 gccttttgtt cataatcagg agctatattc atacctccat ctgtagttat aagtaattta
    23701 tggtattttt ctaactcata taccataaca tgacttaata aactatcagt tctaagacca
    23761 tagtctttat ttaacacttc ttttaatatg acagatgtat ctaataatcc tttcattaca
    23821 aagtcggctt ctttacttga tactaattta actgctattt ctgcacactc tttgatatca
    23881 tccttatcca ctattttgat accacttaaa tcaaatccta tttcttttga tatttctctt
    23941 attttgcctt catttccaac caatatagga gtaattatat ttctttctac agcatccttt
    24001 attgccatta aaacttcttt atcatgagct gctgctacag agagtacaat tttctctcct
    24061 cccttaagtt gttctaaaat atcatctaat ttttttatca aaagtaaccc ctcccttata
    24121 tatatttaac taaataatac catatttgct ataccttttc tcaatttatt ttttatgtta
    24181 taaagaattt atataactaa aagacagttt aatattttat tctgggattt catcaataac
    24241 tcttaaaagc tttgcaggaa caccacctac aatagtattt tctgcaacat tttttgttac
    24301 aactgctcca gcagcgataa ttgcattatc tccaatagta actcctggaa ctacagttac
    24361 attagcacca atccatacat tttttcctat tgtgattgga gaaggataag ttgtaccacg
    24421 tttctctggt gcaataccat ggttaagtgt agccaatacc acattatgcc caatttgcgt
    24481 attatctcca atctcaatcc ccccctggtc ttgaaaatgg caactgctat ttataaaaac
    24541 attttttcct attgtgatgt tttttccaca atctgtataa aatggtggaa acataaaaaa
    24601 tgattcatct accttttttc caattaactc agaaaataaa ctacggactt cttctggttc
    24661 atgataagaa gaatttaact tacttgtgat tttcattgct tcattactca acttaaccat
    24721 aaattcatgt atttctgatt tactagaaac aggttttctt tgattgatat gtgttaaaaa
    24781 ttcattgata ttcattttat acatcctttc aatatgtgct ttaatttatt tgtacattct
    24841 aaataatatt ttaataaaag aaggttttga taatgatgtt gtaagattat atcataatgc
    24901 aaatttgtta ttaaaagagc ttactgaaaa aaataaagta agaatatatt ttatagttgt
    24961 agacgcaaaa tataagataa gtaatgaatt atgtgatgga ttatggatag cacaaaaaat
    25021 tagagaatca gattatatta gtccaatcat atttcttaca aatcatatag aaatgatttt
    25081 agggatcttc gattataggt tagaagttat ggactttata ctaaaacatg atatggaaat
    25141 tgctgaaagt aaaataaaag cttgtattaa aattgctcac aaaagataca ttaaagaaaa
    25201 aaattatcgc tctaattttt ttactatcta ttcagatttc tcactatgga aaatttcatt
    25261 tgatgaagtt atatattttg aaacaagcac tattccacat aaaataaaat tagtgactac
    25321 ttctagaata tttgaattct ataaaagttt aaagtcttta ccagatttag atgcatgctt
    25381 tatccgtgta cataaatctt ttgtagttaa taaatatcat attgtttctc tcgacctaaa
    25441 gaaaaataat attaaaatga
//
LOCUS       DAGSWI010000053        25034 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.053, whole genome shotgun sequence.
ACCESSION   DAGSWI010000053 DAGSWI010000000
VERSION     DAGSWI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.053"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            71..247
                     /locus_tag="MJF80_001961"
     CDS             71..247
                     /locus_tag="MJF80_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280623.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            421..1551
                     /locus_tag="MJF80_001962"
     CDS             421..1551
                     /locus_tag="MJF80_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBZ0280624.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            1792..2142
                     /locus_tag="MJF80_001963"
     CDS             1792..2142
                     /locus_tag="MJF80_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBZ0280625.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            2150..2599
                     /locus_tag="MJF80_001964"
     CDS             2150..2599
                     /locus_tag="MJF80_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBZ0280626.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            2701..3276
                     /locus_tag="MJF80_001965"
     CDS             2701..3276
                     /locus_tag="MJF80_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBZ0280627.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            3269..4678
                     /locus_tag="MJF80_001966"
     CDS             3269..4678
                     /locus_tag="MJF80_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBZ0280628.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            4862..6295
                     /gene="eutA"
                     /locus_tag="MJF80_001967"
     CDS             4862..6295
                     /gene="eutA"
                     /locus_tag="MJF80_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBZ0280629.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            6313..7677
                     /locus_tag="MJF80_001968"
     CDS             6313..7677
                     /locus_tag="MJF80_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBZ0280630.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            7690..8571
                     /gene="eutC"
                     /locus_tag="MJF80_001969"
     CDS             7690..8571
                     /gene="eutC"
                     /locus_tag="MJF80_001969"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBZ0280631.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            8591..9244
                     /gene="eutL"
                     /locus_tag="MJF80_001970"
     CDS             8591..9244
                     /gene="eutL"
                     /locus_tag="MJF80_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBZ0280632.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            9255..9956
                     /locus_tag="MJF80_001971"
     CDS             9255..9956
                     /locus_tag="MJF80_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBZ0280633.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            9959..11428
                     /locus_tag="MJF80_001972"
     CDS             9959..11428
                     /locus_tag="MJF80_001972"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBZ0280634.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     IPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            11524..11811
                     /gene="eutM"
                     /locus_tag="MJF80_001973"
     CDS             11524..11811
                     /gene="eutM"
                     /locus_tag="MJF80_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBZ0280635.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            11938..12699
                     /locus_tag="MJF80_001974"
     CDS             11938..12699
                     /locus_tag="MJF80_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBZ0280636.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            12712..13341
                     /locus_tag="MJF80_001975"
     CDS             12712..13341
                     /locus_tag="MJF80_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBZ0280637.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     RYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRSLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            13383..14108
                     /locus_tag="MJF80_001976"
     CDS             13383..14108
                     /locus_tag="MJF80_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBZ0280638.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKKDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            14121..14393
                     /locus_tag="MJF80_001977"
     CDS             14121..14393
                     /locus_tag="MJF80_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBZ0280639.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            14386..14937
                     /locus_tag="MJF80_001978"
     CDS             14386..14937
                     /locus_tag="MJF80_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBZ0280640.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            14949..16040
                     /gene="eutH"
                     /locus_tag="MJF80_001979"
     CDS             14949..16040
                     /gene="eutH"
                     /locus_tag="MJF80_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBZ0280641.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            16042..16515
                     /locus_tag="MJF80_001980"
     CDS             16042..16515
                     /locus_tag="MJF80_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBZ0280642.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(16635..16736)
                     /locus_tag="MJF80_001981"
                     /pseudo
     CDS             complement(16635..16736)
                     /locus_tag="MJF80_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            17031..17552
                     /locus_tag="MJF80_001982"
     CDS             17031..17552
                     /locus_tag="MJF80_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBZ0280643.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            17931..19106
                     /locus_tag="MJF80_001983"
     CDS             17931..19106
                     /locus_tag="MJF80_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0280644.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLIFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            19140..20678
                     /locus_tag="MJF80_001984"
     CDS             19140..20678
                     /locus_tag="MJF80_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBZ0280645.1"
                     /translation="MQGALKENKKKSGGGFLGWIERVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            20710..21912
                     /locus_tag="MJF80_001985"
     CDS             20710..21912
                     /locus_tag="MJF80_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBZ0280646.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKNENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(22079..22678)
                     /locus_tag="MJF80_001986"
     CDS             complement(22079..22678)
                     /locus_tag="MJF80_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0280647.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARYIKVQL"
     gene            22848..24206
                     /locus_tag="MJF80_001987"
     CDS             22848..24206
                     /locus_tag="MJF80_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280648.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            24199..24831
                     /locus_tag="MJF80_001988"
     CDS             24199..24831
                     /locus_tag="MJF80_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBZ0280649.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
BASE COUNT        10114 a         2838 c         4529 g         7553 t
ORIGIN      
        1 aatttaaata ttttaaaaga taattttttt gaactaatag gtataacaaa aatttatagg
       61 gggattgcat atggaaatca atgttataga aattttccct aaagataaag ctaaacttaa
      121 taaaatagaa atggataaag ctagttggtt tgtaaatata ataagtaaaa aatatcctaa
      181 agaagcttta aatgaagcat ttagtacttt agaaaaagaa ttaaatataa gtaaagctaa
      241 tacataagct ttgattattg attttatata ctattggttt tatacactat gacttgacat
      301 tatattaatc aaatagtata tttttaaata tagcacaatg aagtgttaaa agagcaatgg
      361 agctccatag ataaagtcta tggagctttt ttgcgtattt aacttaagga ggataggact
      421 ttgaataact ttgagattaa aactagaata aaatttggag aaggctcact aaaagctttg
      481 caagaattaa aaaacaaaaa tgtactaata ataacagacc cttttatggt gaaatcaaaa
      541 acaatagata aaataacatc taatttaata aactcaacat atcaagtttt tgaagaaata
      601 gtaccagacc caccaataga agtagtagta gcaggaatag aagcatttaa atctataaat
      661 ccagacgtaa taatagcatt aggtggtggt tctgcaatag atgcagcaaa atctataatg
      721 gacttttcta aaaaaatatt aaaaattaac gaagtagagt ttatagcaat accaacaaca
      781 agtggaacag gttcagaagt aacatcattt gctgtaataa cagatagtca aaaaaaatgt
      841 aagtatccat tagtgagtga tgatttactt ccagatatgg caatactaga cccagaactt
      901 gtaaaaactg taccaaattt tataacagca gatacaggga tggatgtatt gacacatgct
      961 atagaagctt acgtatctac aaattctaat gacttttcag atgcactagc tgaaaaagct
     1021 ataaagttag tatttaaata cttaataaaa gcatataaag atggaaatga catagaagct
     1081 agagaaaaaa tgcacaatgc atcttgttta gcaggtatgg catttaacca agcatcactt
     1141 ggcataaatc attctattgc acatgtatta ggtggaaaat tccatatacc acatggaaga
     1201 acaaactcta tattacttcc tcatgtaata gaatataatt catcaatagt aggatatact
     1261 aacacaactt actctgctac agctaaaaaa tatgcacaaa tagcagaatt agtaggactt
     1321 gggagtgcta atataagaat atctgttaga aatttagtaa atgaaataaa gaaactacaa
     1381 aaagaaatga atatgccaac taaattgaca caatgcaaat taagtttaga agatataact
     1441 aatgaagaaa gagaaatagc aaaacttgct ataaaagata cttgtacatt aactaatcct
     1501 aaggtaccaa ctgaagatga tataataaac atattaaaga acattaaata actacatttt
     1561 attacatgta aatataatat tatacaaata gggaaaatgt ttttaaaaat ataaattata
     1621 tattgacaaa tataaaacat aaaactataa tgaaagtatc acaaaggcgt gatactttag
     1681 tagaaagcaa tggagcttct taggaatatt aatattattc ttatggagct ccttttattt
     1741 taatcaaata gctttttaat aaactatatt caaagaaagc agggaataaa tttgactgaa
     1801 gaatctaagc aaagagttat tcaagaatat gttcctggaa aacaggttac attagcacat
     1861 atcatagcta accctaatga agatatatat aagaagttag gattagtact tgataaaaaa
     1921 gatgcaatag gaatacttac aataacacca agtgaagcgt caataatagc agcagatgtt
     1981 gctacaaagg cttcaaatgt atctttaggt tttatagata gatttagtgg ttcagtagta
     2041 atatcaggtg atgtaagttc tgtagaatca gcgttaaatg atgttttaga agtcttagga
     2101 aatatgttaa acttctcatc tacaaaaatc acaaggacat aataatacaa tgaagaatat
     2161 aatatttatg ggaaagacag gtagtggtaa aactacctta tgtcaaaaac tagaccagtt
     2221 agaattaaag tataaaaaaa ctcaatcagt ggaactttat aataatgcaa tagacactcc
     2281 tggtgagtat atggaaaata gaaacctata taatgcctta ataacaacat cagcagatgc
     2341 taaaattata gcaatagtgt atgatgccac acaagaagaa aattatatag caccaggttt
     2401 tgccagtatg ttttgcaaag acgtaatagg aattataact aaaataaata aagctactga
     2461 aaacgaagtc aatatagctt atgaaagact atcactagca ggagcaagtc aaatctttaa
     2521 aatagacaca gttgataata ttggacttga aaaattattt aaatatctag aaaaatataa
     2581 aaatatagat gaagtttaag gggaaatatt tttttattca aaaatattat taaagtatga
     2641 taaaataaaa aaatgtataa actattttaa gaagatttaa tctttaagcg aggtaggaac
     2701 atggggaaaa aaatactatt ggttgatgat gaaccactgt taaggatgga tacaaaagat
     2761 atattagaaa gtcatggcta tgaaataata ggcgaagcaa gtgatggatt tgaagctgtt
     2821 gaaatgtgta aaaaacacaa acctgattta gttataatgg atatagagat gccaatactt
     2881 gatggtataa aggcaggcaa aataatatgt aaagaaaata tagctggtgg agtactttta
     2941 ttaacttcat ttgaagataa agaatacgta gaaaaggcta aaagtatagg tgcttatgga
     3001 tatttagtaa aaacagcaag tgaaaaatca cttatcccaa ccatagaaat gtgtttaaat
     3061 aaggtaaatg aatttgaaaa aatgaaaaaa gatttagaaa aagtaaattc taaattgaat
     3121 gaaagaaaat tagtagaaag agcaaaaggt atacttataa gagagtttaa gatttctgag
     3181 gatgaagcat acactagaat aagaaaatta agtatggata aaagaactac aatgatggaa
     3241 attgccaaaa tgattataat aggatacgat gactaatacg ataaaaaaaa tatcaactgc
     3301 acaaacatac ttaaataata acgaaattga aaaaattata gaagtatcaa aatccttaga
     3361 tttaatggct tctttttatg aagctgatgt ttttatagat gtactaagta aatttgatga
     3421 tgaagcaatt gttgtggcac atggtagacc taaagaaaag tcaatgtact gtgaaaatgt
     3481 tataggtaaa aaggctttaa ttgaaaatga accaggagtt ataaagtgct taaaaacagg
     3541 agaaagagta agagatatta aagctttaac acaggaatat aaactggtca aacaaaatat
     3601 tcagccaata acactagatg acaaagtttt agcagtacta atagttgaaa aagatataag
     3661 tagtgaagtt aagaatgagt ttaatgctag tgaagacgaa gaaaataaaa atttacatca
     3721 gtttataaaa cttattggaa ataatgactt tttagttgat aatttaaatt cagccatatt
     3781 aatatttgat aaaaatggaa aattaaaaat taaaaatcgt attgcaactg agatatacaa
     3841 aaatcttggg tttggatatg atatacaaga tatgcattac aatgatttag atatagaaac
     3901 caacaccttt gaaaatatat taatggatga aaattatgat gagacaaatg aagtttcaat
     3961 aggtaatatg ttttttaaaa taaagaccat atacatcaaa gatgatgaat ttaaggtttg
     4021 caaaatagta caagatataa gtgatttaaa gagaaaagaa gaagaattag ttttgaaaac
     4081 agtggctata agagaagctc atcatagagt aaaaaataat cttcatacag taatatctat
     4141 aataaaaaaa caaagtagat tatctcaaaa tgatgaagta aagcagtgtt tagataacat
     4201 aataaatagg gtatttgcaa tactttcgac tcattattta ctatctaaag aagttgataa
     4261 taatatatct atacgagaag gtatcaattt attagtgtca aatattcaag gtggctattt
     4321 atttagtaag gatatagata tacgtataac tggtgaagat tttaaaatta gtggagaaaa
     4381 agcaacagca atacttttgg ttataaatga aatactacaa aattgttatg accatgcatt
     4441 ttcggataga gaacatggta ctatacaaat attaataaat aaagataacg actatagaaa
     4501 tattgctatt atagatgatg gaattggatt tgatagtaat aatataaata aaaatagctt
     4561 agggacatat ataatagata gttatataac acagatgtta aaaggaaata tagagagaaa
     4621 aagtagtgag aatggtacag aagtattaat tactatacct acacaaatta atatttaaat
     4681 aataactcat ctacaaagta gtagttttaa ttgagcaaag aggcttaagg ttattaatag
     4741 attaagttaa ttgaatgtat ttataaaaat tataagtata tattcaattt aacaggtatt
     4801 tattataact ttaagcctct ttgcttgttt aagacatact taaattttag gaggtgagaa
     4861 aatgagagaa gagttattaa gtgttggtat tgacattgga acaagtacta ctcagttaat
     4921 tttttctaaa ttaatagtag aaaatactga atcaaatttt tcggtaccta gaataagtat
     4981 agtagacaaa gaaattattt ataaaagcaa agtttatttc acccctctta tttcaaataa
     5041 tgaaataaat ggtcaacaaa taaaagatat agttgaaaat gaatataaaa aagcaaatat
     5101 atctaaagaa gatatacata caggagcagt aataataact ggtgaaacag ctagaaaaga
     5161 aaatgcaaat gatgtacttc atactttgag tggatttgct ggggacttcg ttgttgcaac
     5221 agcaggacct gatttagaaa gtataatatc aggaaaagga gctggagctc atatttattc
     5281 aaaagaacat tcaactacaa ttgtaaatat agacataggt gggggaacta gtaacatagc
     5341 ctcatttaaa aggggagaag taaccgatac tgggtgtttg gatataggtg gaaggcttgt
     5401 aaagatagat aaaataagta aaaaaataac ttatatatca cctaaaatag ataaaatcat
     5461 aaaagataat aatttaaatc ttcaagtagg cacaatggct actgtagaaa atacaaagcc
     5521 aatagtggat ataatggttg acttattaat ggaaagcata ggattaaaga aacagacacc
     5581 tttgtttgat tttataataa ccaataaatc aataagtata gatgaaaata ttaaaaatgt
     5641 ttcattctct ggtggagtag ctgattatat ttactatgat ggagaaataa aagattattt
     5701 tgaatatggt gacttgggaa ttttacttgg gcaagcaata aaaaattgtg aataccttaa
     5761 aaaagtaaat gtagttaaat ctatggaaac aataagagcc acagtggttg gagcaggttc
     5821 tcatactact gaagtaagtg gaagtacaat aacttacact agcgaaaagt ttccacttaa
     5881 gaacttgcca gttttaaaat tatcaattga tgaagaaaca ggcacttcaa aacaaatcag
     5941 tgaagctata aaaaataaac ttaattggtt taaactagaa aatgatttac agaaagttgc
     6001 tatagccatg ataggaaaga aaaatccaac ctttttagat atacaagagt atgctaaagg
     6061 gtttgtagat ggaatgcaac aaatcataga aaatgattta ccttttatag taattgttga
     6121 aaatgatatg gcaaaagtgt taggtcaagc aatatatgca ttattaaatt atgaaaaaga
     6181 aattgtttgt atagacagtg tcaaggttga aaatggagac tatatcgaca ttggaaaacc
     6241 tatagttgaa ggtaaagttt tacctgtggt aataaaaaca ttagttttta attaatagaa
     6301 ggaggtaagt aaatgatttt aaatacaaaa ttatttggac gtacttatca gtttaaaggc
     6361 ttatatgatg taatggctaa agctaatgaa gaaaaatcag gagataaatt agcaggtctt
     6421 gcagcagaat cagctgaaga aagaatagca gcaaaagttg ttttatcaca cataaaattg
     6481 gaggatataa gaaataatcc tgcagtggca tatgaagaag atgaagtaac aagaataata
     6541 caagatgctg taaatgaaaa agtttatgaa gaaataaaaa attggacagt ctctgaactt
     6601 agagaatggc tattggattc aaatactaca agtcttgata taagaagaat atcaagagga
     6661 ttaacttctg aaatgatagc agcagttgca aaattaatgt caaatatgga cttagtttat
     6721 gcagctaaaa agataaaggt tactgctcac tgtaatacaa ctataggaga acaaggaaca
     6781 ttatcagcaa gacttcaacc taatcatcca acagacgacc cagatggaat acttgcatca
     6841 cttcttgaag gtttaacttt tggtatagga gatgcagtac ttggattaaa tccagttgat
     6901 gactctgttg agagtgttac taaagtatta aaaagatttg aagaagtaaa acaaaaatat
     6961 caaataccaa ctcaaacatg tgtattggca cacgttacta cacaaatgga agcaataaga
     7021 aatggtgctc ctgcagattt aatattccaa tctatagcag gttctgaaaa aggaaatgaa
     7081 gcatttggat ttaatggcga cactatagaa gaagctagac agttagcctt aaaacacggt
     7141 acagctacag gacctaacgt tatgtatttt gaaactggac aaggttcaga attatcttca
     7201 gaagcacatc atggaataga ccaagttact atggaagcta gatgctatgg atttgctaaa
     7261 aagtatgaac cattcttagt aaatacagtt gttgggttta taggacctga gtacttatat
     7321 gatgctagag aagttgtaag agcaggactt gaagaccact ttatgggtaa actacatggc
     7381 ttaccaatgg gtgttgatgt ttgttacact aaccatatga aggctgacca aaatgatatg
     7441 gaaaacttag ccatactatt aacaacagca ggatgtacgt attttatggg aatacctcat
     7501 ggagatgatg ttatgcttaa ctatcaaaca acaggatacc atgaaacagc atcacttaga
     7561 gaaatgtttg gactaacagc aataaaagaa tttgatgctt ggatggaaaa gatgggattc
     7621 tctaaagatg gtaagttaac agagttggcg ggagacgcat ctgtattatt aggataggag
     7681 gtatttaata tgaatgagaa ggatttaaaa gcattggtag aacaattagt tggacaaatg
     7741 gttggagaat tagatactaa tgttgtgtct gagactgtta aaaaggcaac tgaagttgta
     7801 gtagacaaca atgcttgtat agatgatata acagaagttg atataagaaa gcaattacta
     7861 gtaaaaaatc ctaaggatgc agaagcttat ttagatatga aagcaaaaac acctgcaaga
     7921 ttaggtatag gaagagctgg tactagatat aaaacagaaa cagttttaag atttagagca
     7981 gaccatgctg ctgcacaaga tgctgtattc tcttatgtag atgaagaatt tataaaagaa
     8041 aataatatgt ttgcagtgga aactttatgt aaagataaag atgaatactt aacaagacca
     8101 gatttgggaa gaaaattttc tccagaaact ataaataata taaaatctaa atttggtaca
     8161 aatcaaaagg ttttaatatt agttggagat gggcttagct cagctgcaat agaggcaaat
     8221 ttaaaagact gtgtgccagc tataaaacag gggctaaaaa tgtatggtat agattcaagt
     8281 gaaattttat ttgttaaaca ttgtagagtt ggtgctatgg accacttagg tgaagaatta
     8341 ggttgtgaag ttatatgtat gttggttgga gaaagaccag gattagttac tgctgaatca
     8401 atgtcagcat atatagctta taagccatat ataggaatgg cagaagcaaa aagaacagtt
     8461 atatcaaata ttcacaaagg tggaacaact gcagttgaag caggtgctca tatagctgaa
     8521 cttataaaaa ctatgttaga taaaaaagct tcaggaatag accttaaata aagtcgaggg
     8581 agtgtagaat atgaaaaatg atgtaattcg tcctaatata ctaggaatta aaataatttc
     8641 aaatataagt ccagaaatgg cacaaaaact agaattaaaa tcacatcaca aaagcttagg
     8701 tttaataact gcagactgtg atgatgttac gtatacagct cttgacgaag caactaaagc
     8761 ttcagaagtt gatgttgtat atgcaaaatc aatgtatgca ggtgctgcaa atgcttcaac
     8821 aaaacttgct ggtgaagtat taggaataat agcaggtcca agcccagcag aagtaagaag
     8881 tggtctaaat gcagtagttg attttctaga gtatggagca acttttataa gtgcaaatga
     8941 tgatgattca atagcatact atgctcactg cgtatcaaga acaggtacat acctttcaga
     9001 ggtagcagga ataagagaag gtgaagcatt agcttattta gtagcaccac cacttgaagc
     9061 aatgtatgca ctagatgcag caatgaaagc tgcagatgtt aagatgtgtg aattatttgc
     9121 acctccaact gaaacaaact ttggtggagc attacttaca ggttctcaat cggcttgtaa
     9181 agcagcatgt gatgcatttg cagaagcagt aaaatcagtt gcagacaatc caacaggatt
     9241 ttaaggatag ttagatggat gctataggct taatagaagt aataggatat gttgctgcaa
     9301 tagaagcttc tgatgcttgt ttaaaatctg caaatgttaa tattgtaaga gttgataagg
     9361 ttggagcagg tattgttact ttaacaatat gtggagatgt cggtgctgta aaagctgctt
     9421 tagaagcagg tgaaatagca gcttccagag ttggtacatt aagaacatca catattatac
     9481 cgagaatcca taatgaagta acaaatgctt tattcaaaac taaagaacct aaagtctgtg
     9541 aagtttcaga agataaagaa aaaaactttg agattacatt ggaaatagct gataatgtat
     9601 cgaaaaattt atcagaaaaa gaaaatgtaa ttttagatgt caatgaaaac agtggagata
     9661 ctgaaaattt atatagtgat atagataaaa caaacaaaaa aattgaaaca gttgatgata
     9721 gtttaacaga agctaatgag gaaaaaatag aaaacactaa tgataaagaa gtattggaac
     9781 aagttaaaat agaaaattcc gattttgata gagatgacct atcaaagaac agtgtaaaaa
     9841 aacttaaagc tatggctaag aaattagatt cttcaataac ttataaagac ttaaatgttc
     9901 ttaaaaaaga agaattaata aacttagtta aaaagcttag tagaagggat agatagttat
     9961 gacactttta gataaagatt tagtatctat acaagaagta agaactcttg taagaagatc
    10021 taaagtggct caagagaaac tttctaaaat gagtcaagag caaatagata aaatagtaaa
    10081 agctgttgta gatgcagcat ttaataatgc tactaaatta gctaaaatgg caaatgaaga
    10141 aactggattt ggaaaatggg aagataaagt aattaaaaat acttttgcat ctatgggagt
    10201 ttatgaaact ttaaagaaca caaaaactat tggaataatt ggtgaagata aagaagccaa
    10261 tacgatagat atagctacac cagttggagt tgttgcaggt cttatacctt caacaaaccc
    10321 aacatcaaca gtaatataca aaactataat atctctaaaa gcaggaaata ctatagtttt
    10381 ctctccacat ccaaatgcaa aaaactgcat acttgaaaca gttaatataa taagtgatgc
    10441 tgctataaag gctggagctc cagaggattc aatattatgt ataactaatc caagtatagt
    10501 tggaaccaat gaattgatga aacataaaga tacaaactta atactagcaa ctggaggaga
    10561 agcaatggtt agagctgctt actcttcagg aacaccagct attggagttg gaccaggaaa
    10621 tggaccagca tttatagata aatcagcaaa tgttaaatta gcaatcaaaa gaataataga
    10681 ttctaaaact tttgataatg gtgttatatg tgcatcagaa caatctatag taatagaaaa
    10741 atctatggaa gaaactgttt taaaagagct taaaaatcaa ggtgcatatg ttttaaatga
    10801 ggaggaaagt gaaagattag caaaattcat attaagagct aatggtacta tgaatccaca
    10861 aatagttggt aaaagtgttg aggtaatagc aaatttagca gacttaacaa atgtacctaa
    10921 aaatacaaaa gttctagttg caagagaaaa taaagttggt cacaatgtag cctattcaag
    10981 agaaaaactt acaccaatac tagcgttcta tgttgaagaa aatgtagaag ctattatgaa
    11041 cagatgcaga gaaatattgt taaatgaagg tgcaggtcat acattctcaa tgcatgtaaa
    11101 tgatgaagaa ttggtaaaaa gatttgccct taatatacca gtatcaagaa tacttgtgaa
    11161 tactccttca gcacttggag gaataggtgc atcaacaaac cttgtaccag cactaacact
    11221 tggttgtggt gcaataggtg gaagttcaac ttcaaacaat ataggaccac ttgatttaat
    11281 aaatgttaaa agagtagctt atggaatcaa agaaatagaa gaacttggaa gagataaatc
    11341 tcaagttgaa aaatcaatgt ttgactcaat ggataaagaa gaattaataa atcttcttgt
    11401 tcaaaaacta gtcaaagaac tagcataact aaaataaaca atgaaactta tggctatcgc
    11461 tataaatttc taaaaatata aactataaaa aactataaaa ttaaattttt ggaggataag
    11521 attatggcaa gtgcaaacgc attaggaatg atagaaacta aaggattagt aggagcaata
    11581 gaagcagcag atgcaatggt taaagctgca aatgtacaat tagtaggaaa agagcaagta
    11641 ggtggaggat tagttactgt aatggtaaga ggagatgttg gagctgttaa agctgctaca
    11701 gatgcaggag ctgccgctgc cgagagagtt ggagaactaa tatcagttca tgttatacca
    11761 agaccacact ttgaagtaga tgctatatta ccaaaggtat cagctgagta atataagaat
    11821 aattataaaa tttaaatttt gattgaaaaa gaggctatct caaaaatgag tcttactaat
    11881 aaactcaatg tattttgagg tagtcttttt tatacatcta ataggagatg tgataaaatg
    11941 ggtgttttaa cagaaaacga agtaaaaaga aaaattggaa aaaacaaaaa taatataact
    12001 gaattttata tagaaaaagg tcaaataata actccttctg caaaatctta tttagctgag
    12061 agaagtattg agcttaaata tgttgataaa ctttcagatg taaacaatga acctaaagaa
    12121 gttgtaaaag aagtagttaa agagaaaaaa tataaatata ctactgtatt tggcgccaaa
    12181 ttggatgaaa aaccagaaca tatgactcaa ttaaatggaa atcttttagt ttttaaagac
    12241 cataaaagaa taatattgag aggaaaaata gattctcttg aatcagaaat actaaaggta
    12301 cagatacttt gtcaaaaact agatatgcca aagcttgtta aggaattgca agaagtatta
    12361 gactttgtaa gaaatataat gagatgtgaa gttttagatg aaaagctagg ggactttaaa
    12421 cttttaggat taactcctca agaacttaga gaacaatctc actatcctaa aaaatacttt
    12481 ggtataggac atgagttccc aagttatgaa atgggagaag tagtagtagc tataaatagt
    12541 ataagaagta atacacgaga ggttgaactt gcagcctatg aagcatttaa gggtgagtat
    12601 ggaaatgttc acagagaaga tttaataaag gcacttaata gattatcgtc agtattttgg
    12661 ataatgatat ataaggttag aactaataag tataagtagt tggaggacat aatggagaat
    12721 ctattaaatg aaatagtgga agaagtaata actagagtta aaaaagaagc atttatagaa
    12781 gttgaagcat caggaagaca cgttcactta agtagagaag atgtcgataa attatttgga
    12841 gaaagatata ctcttacaaa actaaaagac ctatctcaac caggtcaata tgcatgtaag
    12901 gaaagagtta caataactgg tcctaaagga agtataaaaa atgtagttgt acttggacca
    12961 tgtagaaatg aaactcaggt agaaatatct ttaacagatg gttcaactct tggactaaaa
    13021 gctcctataa aacaaagtgg agacttagaa ggtactttaa gtattaaaat atcaacacaa
    13081 tatggtgaag ttgaacttga taaggggcta atggttgcta aaagacatat acacatgact
    13141 cctaaagatg ctgaaaaatt caatgtatgt gataaagaga tagttgaagc taaggtgatg
    13201 ggtcaaagat ctttaacatt tgatgatgta gtaataagag tgagtgacag ttttaaaact
    13261 tatatgcata tagactatga tgaagccaat gcatgtggtt attcaaaagg aactgttgct
    13321 aaaataataa agaaagcata aatttaacct actatataaa ttacctctag ggaggattta
    13381 atatgaatta tgataacctt gtagatttaa taacggaaga aatctataaa aaaataaata
    13441 gtaatgaaat aaaaatatct aataaaccta aagctgtgat tgtatttgaa caagataaca
    13501 ataaatttaa cttattaaaa gatgaatttg aaatagtaga atttgataaa agtataaggg
    13561 aatgtgaaat agtgatagtt tcaagacttt gtatgagggg actatcgaat atagcacttg
    13621 gaaattcaac aagtgatgaa gaaagattta tactaaaaat gattatgaag ggcaaaaaag
    13681 tatatgtcct agatgaagga attgaataca aaaaatataa ggatactgca ccaaaagcat
    13741 tatataagaa atttatgtca tttgaagatg aaatatgtaa atttggtgta gaaattataa
    13801 aagacttaaa tgttataact aaaaataagt taaatataaa gaaagaacta gaaagtacta
    13861 gaggttctaa aaaaaaagat acaaaagttt tagataaaga tgttttaaat ttaaccagtg
    13921 tagaaagtgt aaaaatagat aatgaattta gtttagattt aaggaataaa aaacttatat
    13981 cggaagctga cttaagaaaa ccaactataa atggagttaa aaacatatta gttaataaaa
    14041 aaagtataat aacaccatta gctgtagatt ttatgagaat acaccatctc aaactaaaga
    14101 aactgtaagg aggtaatttt atggatatag gaaaagttgt aggtaatgtt tgggctacaa
    14161 gaaaagatga aaaattatgt ggccaaaaac ttttagttgt aaaaatttta aaaactaaag
    14221 acacatacaa ggaaggcttc atggttgcag ccgataatgc aggagcagga aatggagact
    14281 tagttcttat atccaaaggt tcctcagcta gagagtcgat tgaaaaatct catgttccag
    14341 tagatgcaac tatagttgga gtagtcgatt catttgaggt gtttgatgaa taaaagtata
    14401 ggagccatag agtttaaaag cattgctaaa ggtatagaag tatccaatga aatgataaag
    14461 aaatcttcag ttgatgtatt gtacttaaaa agtatttgcc caggtaaatt tctaataata
    14521 gtaggtggag aaacttctta tataaatgaa tgtgtagatt atggaataaa acttggagaa
    14581 ggatatattg tagataactt tgtaataaat gcaatttcac aagaaatatt agatggattt
    14641 aaaaataagt atcaaaagct agatagtata gtctctatag gcgttgttga aagtagtaaa
    14701 gtatgcgctg gaataaaaat gcttgataag actttaaaat ccggtgactt agtattagta
    14761 aaacttcaat tatcttttgc tataggtggc aaattagtat atatagtagc aggagattta
    14821 agtagtttag aatatgcatt aaaagaaagt gaaaatgtag taagagaaaa agaagtaata
    14881 tataagactg taataccatc agtagacagt caaataataa aaagtctaat aaaataatgg
    14941 ggggaattat gagtataaac gaaattataa tctatgtaat ggtagtcttc atggtacttg
    15001 gagctataga taaatgtata ggtaacaaat ttggtcttgg agaacaattt gaagaaggta
    15061 taatggcaat ggggtcactt gcagttgcaa tggttggtgt tatatgttta gcaccagttc
    15121 ttgctgatgt attaagacca attgtagtac cagtatttaa tatacttgga gctgacccag
    15181 ctatgtttgc tggaagttta ttagctaatg atatgggtgg tgcgccactt gcacttgaac
    15241 ttgcgaaaga ccctaatgct gggttatttg gtggattaat agtaggtgct atgatgggac
    15301 ctacaatagt atttataata ccagttgcgc ttggaataat tgaaaaagaa gaccaaaaat
    15361 tcttagcaac aggaatactt gcaggtataa taacaatacc tattggagca ttcgttggtg
    15421 gattagttgc aggattcgag gtaatgatgg ttcttagaaa cttagtgcca ataataatat
    15481 ttgcagtagt tatagcactt ggattattaa aattcgaaaa tgctatgata aaaggattca
    15541 catactttgg aaaaggtgta gtaatagtta taactttagg acttgctgct gccatagttg
    15601 aagctttaac aggaatagtt gtaatacctg gaatgaaacc aatcagtgaa ggtatagaaa
    15661 tagttggaga tatagcaata gttttagcag gagcattccc attagtgttt gttataacaa
    15721 aggtatttaa taaaccatta atggctttag gaaaagttct tggaatgaat gatgtatcag
    15781 cagcaggcct tgttgcaagt ttagctaact gtataccaat gtttggtatg atgaaagata
    15841 tggacaatag gggaaaaata ataaatgtag cattctctgt atcagcagca ttcgtatttg
    15901 gagaccactt aggatttacg gcaggattta attctaatat gataacacca atgatagttg
    15961 ctaaattagt tggtggtata acagcagtat tgttagctat ggttatcgca aataagacac
    16021 taaagaagga gaatgtataa gatggatata tcaaatatag ataaaaattt aattgaaaca
    16081 ttagtaagac aaataataga agaaaaaata agtggaacta aagacacagt agattttgtt
    16141 agaaataaag acattagtgg aataacttct ataaagttac caactgtaaa agttagtgaa
    16201 tctgatagat tagatacagg gaatccaagt gatgtagttt acacaaaaga tttatttaca
    16261 ttagaggaaa gcccaagatt aggttgtggt atgatggaaa tgaaagaaac aacttttgat
    16321 tggactctaa actatgatga aatagattat gtaatagatg gaactttaga tataataata
    16381 gatggaagaa aggtttctgc ttcttcaggt gaattaatat tcataccaaa aggaagtaag
    16441 atacaatttt ctgtacctga ttatgctaga tttatatatg taacatatcc ggcagattgg
    16501 gcatctcaaa attaataaaa atcaagttaa gttaataata atcaaataaa taatttaaaa
    16561 tacaaactct taaataagca cacatattta aaaaagctat gaattaaaat tcatagcttt
    16621 ttttataaat aatgttatat tttagacgta ttttttctat atttactggg agacatatta
    16681 tattttttat taaataatct aataaaatgc tctacatttg gataaccaat actattttac
    16741 tgataatctt ttccttatca ctaatcttac attttaaaat attattttta ctcgtaatat
    16801 tatcataaga atttgtttct tatttaaaac taattagaat tataaagttt attgccatgt
    16861 tattaataaa ataaatccag ttaaatcgag tgctagttaa agtaataaga aacttttaca
    16921 atggtttcct ttatcgattg aaataaaagt taatatggta taatatctaa agagagaatc
    16981 ttatcaatta gaatatgcat agaagcaagt ggcttaaaag gagacgttat atggaagaga
    17041 tagtaaagaa agaacaagca agtatgaatg atattttttt gtatcttaaa gctaaatcaa
    17101 actcattata taaatttgta atattatata atgagtttat caataaacct aaagattatg
    17161 gaactgggga acttataaac atggttgaag tacatatgct aacagcgatt gaagaaaatc
    17221 caggaataag tccaactcag ttagctaaaa tatggaacag aactaaaggt gcaatatctc
    17281 aaactatatc taaattggag aaaaagggtt atataactaa agaaaagaaa gaaggaaatg
    17341 caaaaactat attactatat ccaaccacat caggagtaga gttaagtaag gcacataaac
    17401 tatatgacac catagatgtt acaagaacat ttgaatactt aatcaaggag tgtacattag
    17461 aggagataga tagtttttat aaagtaattg atttgtttat taatttatta gaagaatctc
    17521 cgcaggaaaa taatgattct gaaaaagtgt agctaagatt gaagttgaat aaagagaaat
    17581 agatattata gaagtagtac ttaagtacac attatataaa aatataatag gtatatgatt
    17641 aaaaagttta gtggtttaaa gtttaaccac taaacttttt taatgtctat aatctactat
    17701 tatttaattt actaaaaata aaagaactca ttatattaag ataaaaatag aaaaataaat
    17761 agaaaaatac tataatatat cagcttaata cagaaaaaag ttaaaaaatt tgagaaaaat
    17821 aaaaatgaaa acgttttata ttgacataga gaaaaagtag tgataccatt taaatatagt
    17881 ttagtagcta aactatattt aaattataat aactatttta gggggatgtt atgaatttaa
    17941 aacaattaag tgaaaaatac gaagaatatg taattcaaat gagaagagaa tttcataagt
    18001 atccagaatt atcttttaaa gaaataagaa caacccaaag aataagggaa gaattacaaa
    18061 aaataggggt cccttttgta tctattgaac caaatatcgt aatagctact atacaaggca
    18121 aaaatgaagg taagacaatt gcaattagag gagatataga tgcgcttcca atgcaagaag
    18181 attcagatgt tgaatacaaa tcagaatatg atggaataat gcattcttgt ggacatgatg
    18241 ctcatggagc tatgttacta ggacttgcac atatgctaaa tgatataaga aaagatttaa
    18301 aaggtagagt attattaatt tttcaagcag ctgaagaagt tggtggcgga cacaaggaga
    18361 taatacagta ctttaaggaa aatggaggac cagatagatt aatagctact catgtttggt
    18421 ccgatattga agctggtaaa atttctgttg aaccaggacc tcgtatggct ggtgggtctg
    18481 gttggagaat agacataaca ggtagaggag gtcatggttc tagaccagat caaagcatag
    18541 atcctataaa gccagcttgt gatatagttt taaaagtatc ttctatacca gttaatcata
    18601 taagtgtact agaacaatgt gttgtacatg catgtgcaat acatggagga actacaatag
    18661 gaaatgttat tccaaacaat gcagaggtaa taggtggata tagatatttt actgaagaat
    18721 caggcaaaaa agtgtttgac attataaagc aaatgagtaa tggtgttgga caagtttatg
    18781 gagtggatgt gaaggttact catactggag gcataggacc tgttatcaat gatgaagaag
    18841 cagttaagat ggctaaagaa atagtttcta atatagatgg attggagtta gattcttatg
    18901 agccaatatg tgcatcagat tgttatggtg aaatattaaa agagtattct ggtttttatt
    18961 gttatctagg ggtaggtaac aaagaaaaag gaataatata tgcacaacac catcctaagt
    19021 acaatataga tgaagataca ttaaagttag gttgcgaatt tatggctaaa tatgcagttg
    19081 aatttttaaa taaaaataat aactaaaata aatcgaattt tataaatggg gggatgaaaa
    19141 tgcaaggtgc attaaaggaa aataagaaaa aaagcggtgg aggattttta ggatggattg
    19201 aaagagttgg gaataaaatg ccacatcctc tagcattatt tacatatatt acattgatag
    19261 tagtagcatt atctgcaata gcaagctttt taggtttttc agcaacaagt cctgctacag
    19321 gaaagttaat aaaagttatg aacttaataa gtgcaaaagg tttagtacta tttatgcaag
    19381 agtttgttaa gaacttccaa aacttccctg tgcttggagt agttttagtt atggcagttg
    19441 ccacaggagt ttgtgaaaag acaggatttt tttcaactgc cataaaaatg agtttatcaa
    19501 aagttaaggg gaatgcggtt gtatttataa tagcttttat aggagtattt gctaatcaag
    19561 ctggtgatgc tgcttttgta ttagtaccaa ctcttgcagg agcaatattt tatggattaa
    19621 atagaaatcc attagctggt atattctgtg gatttgcatc agttggtggt ggatttgcaa
    19681 cagctgtaat tcctggggga tgggacgtta cgctaacgcc aataactgta tcagctgctc
    19741 aaactattca acctgcattt gatatgacat tattagctag ttattatgca ctatttgtat
    19801 ctgcgtttat agtagctaca gtatcaacaa tagtaacggt taaatttata gaacctaaat
    19861 taggtaagta tactggtaaa ccagaaggta tagatgataa tcaaggtttt gaggtcacaa
    19921 aagaacaagc aaaagcagca aagttagcag gttttacagt tttagcttat gtagcgttat
    19981 taattgcttt gtgtatacca gctaatagtt ttttaagaag tccagaaggt tctttaatat
    20041 ttggagcacc acttatgtct tgcttacagt tttttatagt aatactattc ttcttgccag
    20101 ggattgtgta tggaatgagg gttggtaaaa tcaagactgt aaaagactta aatgatatat
    20161 tatgtcaatc aatggcagca atggcaccgt ttatcgtact tgcaatagtt ataggtcaat
    20221 tcttaacgct attctcagtg tctaatttag cacaggtact agctataaaa ggtggagcat
    20281 tactcaactc attgccacta ccaccacaag ctataatact gctattctta gtactagtag
    20341 cgtttataaa tatatttgtt ataagcggaa gtacaaaatg gatgatattt ggaccagtat
    20401 ttgttccaat gttaatgcaa ttaaacatac atccagcatt tactcagttt gtatatagat
    20461 taggagattc tgtaaccaat catttatcac cattaaatgc attctttata atacttttag
    20521 caacagtcca aaaatacgat aaaaatgcag gtatgggaac tatattctca gctatgatac
    20581 cttacactat aggttacttt gtagtacttg cagttttagt agtagtttgg atgacaatag
    20641 gtataccagt aggaataggt ggacaagttt ggttataaaa catatttaat aattttggga
    20701 ggaaagaaaa tggaaacaac tgaaataaga tacaattttg atgaagtaat agatagaagt
    20761 ggaacaaact gtcttaaata tgatcttaag agtcaattta tgccagaatc tccagaagat
    20821 gcactaccac tatgggtagc agatatggat tttgcatgtg cccctgagat aattaatgca
    20881 atgcatgaaa gaatagacag aaaaatattt ggatatagct ctcaacaaac tgatgattat
    20941 tatgaagctg tatgtggatg gtttaagaga agatttgatt ggaaaataga aaaagagaat
    21001 atattcttta gcccaggggt tgtgcctgcg ttaggatttt tcacacaaat attaacacaa
    21061 aagggtgatg gtatcataat agaaaagcct gtatactatc cttttgaaga taaaataact
    21121 aacaatgaaa gaaaagtagt taatagtcct ttaaagtttg aagatggtca ttattctatg
    21181 gattatgatg gatttgaaga attagctaaa aatgaaaata ataaggtgtt tatattatgt
    21241 agtcctcata atccagttgg tagagtatgg aaaaaagaag agcttagaaa aatagtagac
    21301 atatgtaaga aatatgattt gtggataata tctgatgaaa tacattgtga tattattcgt
    21361 aaaggtgaaa agcatatacc attagaagta gcatgtcctg attataaaga tagaataata
    21421 acttgtacag ctccaagtaa gagttttaac ttagctggta tgcaattatc aaatatagta
    21481 atcaataata aggaattaca agaaaaatgg atgcttgaaa cagattctaa attagctata
    21541 ttcctaccta atccattcgc aatagttgct actaaagttg cttataatga ttgtgaagat
    21601 tggatgaatc aagtaaatga gtatatagat ggtaatatta aatatgtaga agattttgtt
    21661 aatgagtata tgcctaaagt aaagataata aaatctcaag gaacttactt agtttggtta
    21721 gactttagag ggtatggatt agatgcaaaa cagcttgaat atgtaatgct taacaaggcg
    21781 aaggtattgc tagatgaagg atacatattc ggagaagaag gagcaggatt tgaaagaata
    21841 aatgttgctt cacctagaac aattatagaa gagtgtatga atagaataaa atgtgcattt
    21901 gaaggattat aatagtaata tctaaataat atatttgtgt attttaaaaa aagactatgc
    21961 taaaaaaata tttcaaagca tagtctttta aagttttatg atgagtttaa aaaatttcaa
    22021 aacgttacta ttcacttata tgtttatatt agaaataatt ccacaatata ctcattttct
    22081 aaagttgcac ttttatatac cttgcttcac caatatcatg tgtaaccaaa atagtagact
    22141 tattctcatt cttaataata ctataaacat catcacaaac taataatcta gtctgataat
    22201 caagagctga aaaaggttca tctaaaagta gaatatctgg atttacagaa agtgttctaa
    22261 taagcgcaac tctttgtctc atacctccag ataattcttt tggatacata cttctaaaat
    22321 cccataatcc ataagtcttt aaaaggcttt caactctatc taaagatttt ttatcttttt
    22381 tgccttgaat ttccaatcca atagttatat tatccattat atttctccat tcaagcaaat
    22441 tatctttttg aaacatatat ccaatatttc cagtaatctt tatatcacca ctagtcggtt
    22501 ttaaaagatt agtaagaata tttaatattg tagatttacc acttccagaa ggaccaagca
    22561 aagttaatat ttctccttct ttcaagttaa aattgacatc ctttaatact tcaagctcgc
    22621 cttccttagt gtagaagttc atatttatat ttttaacttc tactatgttg tcattcatta
    22681 tgccaccccc tataactttt agctcgtata tattatgtta ttaacaatat gacctatttg
    22741 taccattttt cataaatata atataaatat tttgtttatt tacgtatttt cttagtaact
    22801 tgtacgtata aattatagat aaataaatta taaggtaggg gataaatttg agtaacataa
    22861 taacgaattt aatagagtca atatttacta atccaataac tatattgttt ttacttgcaa
    22921 taactgtgta ttctgttttt aaaataatag aaaataacag agtatattca tttgtaagtt
    22981 caaaattaga cgaaatcaat agagactata agacaagtga gttttataaa aaggtaaggg
    23041 atgactacta tacatatagt aaagaaaatc cttatgcaga cgtaaatata acttcattta
    23101 tagaggaagt tgtatctgat ttaaagcata ataatttgcc attgttagaa aaaattagaa
    23161 gtataaaaaa ttcatcttca ataagtatat tacttggggt tttaggtacg tttgttggtc
    23221 tttctacaat gcttttatgt gttgatacta aagacattat aaattcactt ccttcaacta
    23281 ttagtagtat gcaaactgca tttacaacta gtatatttgg agttgtattt tctctaatta
    23341 taggttactt tactaagata aaagattgtg aacatgtttt gatacaaata atgttaaaat
    23401 ctgaaaattt actaacatct gaaataactc attttaagtc agaaagaatg gatttaaaag
    23461 tagaagaggt aaaaaataca ataaaacaaa taagtaagtc tatagaagct attgaaaaat
    23521 ttgataaaat atctaaagat ttaaatgact ttaatgatga atttatcagt ggtatagaag
    23581 ctttaaaaag ccttttagaa ggttctcaaa gttctattaa gacatttgac cagagcgtaa
    23641 gaaaattaga taaacagttt aatattttaa atataaaatt tgtaaagctt tttgataaat
    23701 atgataatca agataatata aataaagaga ttttatttga tataaaagaa tcttcaaaaa
    23761 atatatacaa tgccactgaa agccaattca aaattagaga ttatataaaa aatataaatg
    23821 caggatttgc tctatatgaa agaagtgctc aagacttatt aactaaatta atgacccatg
    23881 aaaataaaat ctctcaaaat caaaaaatcc ttttagatga aaagtttacc ttagatgact
    23941 caataaaaaa tctctcaagt attattgaaa acttttctaa tgacctacaa gttaaactgg
    24001 atatgatgtt tgaaaattct ttagatattc aagataagtt agatgttatg tttaataact
    24061 cttttatgaa tgatgaagtt cctcttgata gtgaagaatt atttaaggat gatgttaata
    24121 atgtatttaa tccttttagt gaagaaattt atgagataga ggataaagaa ataaaggtca
    24181 taggtgagga tgagttaaat gaataataaa tatagaagaa ctgttaacag agaatttgaa
    24241 aagagtagct tttggccaac atttacagat ttattatcta ctgtattgat ggttgtaatt
    24301 cttatcttat ttagctcaga aagtatttca ggttctgttg aacaagattt agctaagaat
    24361 gtcaatgctt ctgttgaaga aacttttaaa aaaagtggaa tacctgtgaa ggtagataaa
    24421 tctaatgggc aagtaacttt tggtgaaaaa accatgtttg atgtagatag tgatgtttta
    24481 aaaccagagg ctaaagaaat gctaaaaatg tttgttccaa agtacataga gactatttat
    24541 aaagattatg gtgactatat atccaaaata gttatagaag gtcatacaga tgatgtggga
    24601 agttatatat acaacttaga tttatcgcaa agaagagcat atagcgttgc taaatttata
    24661 gtgggagatg aaataggtga ctataaatac aaagataaag tgactaagca tattattgca
    24721 ataggaaggt caaaagctga actcatcaaa aatggtgaca atagcgtgaa tagagatgca
    24781 tctagaagag tagaactaaa atacgaaata aatattaatc aaaataaata attaaatttt
    24841 tataaaaaaa aagaaggcag ttggtgtgag ctttaagcct tctttaagga aaaagtttat
    24901 ttatttttgt tgatacttaa catattatag tttttagaag cagtttgcaa tatattaaac
    24961 gtaaatggtc gtttaaaatg cataactgga tactactgat gtaaaatatt tcattttata
    25021 taatataaaa atta
//
LOCUS       DAGSWI010000054        24135 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.054, whole genome shotgun sequence.
ACCESSION   DAGSWI010000054 DAGSWI010000000
VERSION     DAGSWI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.054"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            248..694
                     /locus_tag="MJF80_001989"
     CDS             248..694
                     /locus_tag="MJF80_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280650.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVS"
     gene            1075..1992
                     /gene="pstC"
                     /locus_tag="MJF80_001990"
     CDS             1075..1992
                     /gene="pstC"
                     /locus_tag="MJF80_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBZ0280651.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            1994..2818
                     /gene="pstA"
                     /locus_tag="MJF80_001991"
     CDS             1994..2818
                     /gene="pstA"
                     /locus_tag="MJF80_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBZ0280652.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            2837..3601
                     /gene="pstB"
                     /locus_tag="MJF80_001992"
     CDS             2837..3601
                     /gene="pstB"
                     /locus_tag="MJF80_001992"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280653.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            3629..4282
                     /gene="phoU"
                     /locus_tag="MJF80_001993"
     CDS             3629..4282
                     /gene="phoU"
                     /locus_tag="MJF80_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBZ0280654.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            4440..5309
                     /locus_tag="MJF80_001994"
     CDS             4440..5309
                     /locus_tag="MJF80_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBZ0280655.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            5396..6832
                     /locus_tag="MJF80_001995"
     CDS             5396..6832
                     /locus_tag="MJF80_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBZ0280656.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            6880..7707
                     /locus_tag="MJF80_001996"
     CDS             6880..7707
                     /locus_tag="MJF80_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBZ0280657.1"
                     /translation="MKIKDNKAVFHVFIRIFIIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    8008..8270
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8346..11012
                     /locus_tag="MJF80_001997"
     CDS             8346..11012
                     /locus_tag="MJF80_001997"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBZ0280658.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     gene            11437..12147
                     /gene="rgaR"
                     /locus_tag="MJF80_001998"
     CDS             11437..12147
                     /gene="rgaR"
                     /locus_tag="MJF80_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBZ0280659.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            13193..14224
                     /locus_tag="MJF80_001999"
     CDS             13193..14224
                     /locus_tag="MJF80_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBZ0280660.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALLMKVADVVRDCL
                     DIEDNNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            14357..15655
                     /locus_tag="MJF80_002000"
     CDS             14357..15655
                     /locus_tag="MJF80_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBZ0280661.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(15769..16014)
                     /locus_tag="MJF80_002001"
     CDS             complement(15769..16014)
                     /locus_tag="MJF80_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBZ0280662.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            16162..17028
                     /locus_tag="MJF80_002002"
     CDS             16162..17028
                     /locus_tag="MJF80_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBZ0280663.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(17032..17568)
                     /locus_tag="MJF80_002003"
     CDS             complement(17032..17568)
                     /locus_tag="MJF80_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBZ0280664.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(17759..17962)
                     /locus_tag="MJF80_002004"
     CDS             complement(17759..17962)
                     /locus_tag="MJF80_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBZ0280665.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            18253..19017
                     /locus_tag="MJF80_002005"
     CDS             18253..19017
                     /locus_tag="MJF80_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBZ0280666.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            19406..20719
                     /gene="prdC"
                     /locus_tag="MJF80_002006"
     CDS             19406..20719
                     /gene="prdC"
                     /locus_tag="MJF80_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBZ0280667.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      21123..21208
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            21790..23937
                     /locus_tag="MJF80_002007"
     CDS             21790..23937
                     /locus_tag="MJF80_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBZ0280668.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDSQTPDVEKPSDGQMPDVEKPLDEQTPEEEKPLEEK
                     IVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEEDVKADEQSEINQVFTSKDSFISL
                     WYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASSKDDKYVIE
                     GEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQENQNEEAVKSILYAGSEFDGFGY
                     KQQFNLGGEENELMTYSATQSAIWIILGQMDEQERLKQYQGSINLCDKLIERAKTEEE
                     RAEYQKRKEVAINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYET
                     EAVALVGYSGVVKLKLPDGVTAYDVDGNIIGTGEVEISTQQKFKLKSVGKPDAKANIS
                     AVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPT
                     DDDVVNPPVPPIDDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPP
                     VPPTEDAVLNPPVPPMEDAVLNPPVPPTDDTVLNPPVPSVSDTVEKTPELSRDDTIIK
                     SPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
BASE COUNT         9633 a         2578 c         4281 g         7643 t
ORIGIN      
        1 aaataataag attaagaatt tcttaataaa tccagaagat gctttcgatt atataatatt
       61 ttatattttt atattattaa ttatatgtgt tgcaattgct gcacctactt tttctggtga
      121 gtatcagaca caatcagata gcatccttcg ttgtacgaaa catggacata ttcgtctagc
      181 gtttactaaa atattagctc tattttctat atttataata atgtttgtta tatgtatttc
      241 tatccatttg gcaatttctg atttagcttt tggtactgaa tgtttgaaaa cctcatttca
      301 aatgttgttt tctgtaatta gtctagtaaa tataaattta ctacaagcac agattcttat
      361 agctattggt ggtctaattt caatacttgc aatggttagt ttaacattat ttttatctgc
      421 aaaatgtaag gattctctta cttctatgct gattgctttt tctatttgct taattcctat
      481 gggaataggt gttagttgga ttggatacct attaccacca gggggaattg gttttaagat
      541 tagtttatta tatcaaatga catcatttaa tttcttacac ttaggaggta tgagtttttg
      601 gacaccacac attatactgt ttttctcagt gatagaaata cctattttta tatttttggc
      661 tattcgtacc tattgcaaac atcaagtatc gtaacaattt gatggaaata atagtgatat
      721 aaactagagg atacaataat tatatatgta gtataaaagt taaatttagt taacaaatct
      781 taatagcaga tataggtaat aaattataca taaactgtat tattatctat atctgctatt
      841 ttaatataga gaaacaacag tatattgtaa agtaaatgtc aaatatagtt ttaaaagtaa
      901 aattaatgtt aaaaagtaaa tataatttta cacttgctta acttaatcct aatatagatt
      961 taacttaaaa aaagtagaat aaaagtatag gaaatttgat gaagacgaat tataaaaaat
     1021 taaattttga atacagaacg aaaatcgata agtatatttt aatgggaggg tattatgata
     1081 gtaaaatcta gattagtagg tttagaaaaa aatgacaata atggcaataa gacaaaatac
     1141 gtaattgaaa aagtagcaaa aaatattttt ctacttagtg ctttagtagc agttgcaagt
     1201 ttacttttga taataggatt cgtattttat aaaggattaa gaccatttat atttgaagga
     1261 tattcattta tagacttttt aactggtgca gattgggttc cttctgccaa taaatttgga
     1321 atatcatcta tgatagtagc atcaatattt gcaacacttg gagcacttgt gataggagta
     1381 cctgttggta tattaacctc tgtatttata gcagaagtag cacctaaaag attagcaaaa
     1441 ataatgtcac cagcagttga acttttagca ggtatacctt cagtattata tggtgtattt
     1501 ggtcttgcag tgatagttcc aactatacaa agtatattta atcttccaaa aggtcaaagt
     1561 ttgttagcaa tagtaatagt attagcagta atgatgttac caacaataat atctgtatca
     1621 gaaacagcaa taagagcagt accaaaagca tacaaagaag gttcattagc acttggtgca
     1681 tcaaaaatag aaacaatatt caaagttgta ttacctgctg caaaatctgg agtattagcc
     1741 gcagtagtgt taggagtagg aagagcttta ggtgaaacaa tggcagtaat acttgttgca
     1801 ggcaactcac cagttatgcc aactgcaata acagatagtg taagaccact tacaactaat
     1861 atagcacttg aaatgggata cgcatttggt acacatcaag aaatgctatt tgcaactgga
     1921 gtagtattgt ttacatttat attaatttta aacttagttt taaataaact ttcaaataag
     1981 gcggtaaatt aagatgagaa agttcaaaga gaatctattg aagtcattag tatatctatc
     2041 agcactattt acaatagtat cattagtagt tatagtggga tttatattta taaagggaat
     2101 tggcaacatg aattttaatt tcttatttag caattattca gcatcaggtg atggtggtat
     2161 acttccaatg ataattacaa cattatatac agtagtggtt tctatagttg tagcaactcc
     2221 tataggaata ttggctgcta tatatttaca agaatatgct aaaaaaggaa aagcagttac
     2281 agctataaga tttgcaactg aaagtttatc agggatacca tctatagtat atggattatt
     2341 tggaggaatt ttctttgtag ttacattaaa aatgggatat tccatagttg caggttcact
     2401 tacagttgca ataataatat taccagtaat aattcgtaca acagaagaag ctttaaaaac
     2461 agttcctcaa gcttatagag aatcatcatt ggcattagga gctacaaaat ttcaaacttt
     2521 atataaggta gtacttccaa gtgcaatacc aggaatttta tctggagtaa tcttatcagt
     2581 tggtcgtata attggagaat cagctgcaat attacttaca gcaggtactg tagccaagat
     2641 gccaggtgga atatttgata gtgcaagaac attaactgta cactcatact tactgactaa
     2701 agagagtgga gatatagcaa cagcagccag tataggaatt gttttaatag taattgtatt
     2761 agcactaaac atgctagcaa gatttgttgc taagaaatta aataaagcta attattaaat
     2821 ttaagaggtg tgaataatgg aattaatcga taagataaaa atgagtgtaa aagatttgga
     2881 tttattttat ggtgataagc aagcattaaa gaaaataaat atggatataa aagagaataa
     2941 ggttactgct ttaataggtc catctggatg cggaaaatca acttttataa gaacgttaaa
     3001 tagaatgaat gacttaatcg aagatgtaac aattaaagga aacatttctg ttgatggaga
     3061 ggacatatat acctcagatg atgttataaa tcttcgtact aaagtaggaa tggtatttca
     3121 aaagccaaat ccatttccta tgagtatata tgataatgtg gcatatggtc ctcgtacaca
     3181 tggactaaga gataagaagc aattagataa aattgtagaa gaaagcttaa aaggtgcagc
     3241 tatttgggat gaggttaaag acaggcttaa atcatctgca ctaggacttt ctggtggtca
     3301 acaacagcgt atatgtatag caagagcaat tgctatgaga ccagaagtta ttcttatgga
     3361 tgaacctaca tctgctttag acccaatttc aacattaaaa gtagaggaac ttatagagga
     3421 tttaaaaaaa gactacacaa tagtcatagt tacacataat atgcaacaag ctgctcgtat
     3481 atcagatgaa acagcttttt tcttaaatgg agaggttata gaatttagcg atacaaaaac
     3541 tatgtttacc actcctgtag ataaaagaac agaagactat ataacaggta gatttggtta
     3601 aaatatataa ctataaagga gttagacaat ggtcaataat agtttagata taagtattga
     3661 tactttaaaa gaacaaacag tgcttatgat ggaaaaatgt gaagaagcaa tagaaaagtc
     3721 tgtgacttgt atgatagaga aggatttaga gggtgcaaga aaagtagtag aagatgatga
     3781 agaaataaat aaattaagag aagaaataag agataagagt atagagctaa tagtattaaa
     3841 gcaaccaatg gcaagggact taagatttat ttattcatta ggtaatatat ctatagagct
     3901 ggaaagaata ggagattatg cagcaaatat agctagagaa atgttgaaga taggtggaga
     3961 ggcttatata aaagagctaa tagatattcc taagatggca aaagaatgta agtctatgat
     4021 gtctcagact agagaagctt taatcttatt tgatgcaagt atagcttatg atgttggaac
     4081 taaagatgat attgtagatg gattgtatga acaagtacaa attgattgtt taaaaataat
     4141 gcatgaaaac tcaaatacca tagaccaagg tgtaaaactt ctacttatag gaagatacct
     4201 tgaaagaatt ggagaccata taacaaatgt atgtgagaag ataatttttg caattgaggg
     4261 taaaatggtt gagataggat aaaaaactgc tttaaagcag tttttttatt gtctaaatta
     4321 agaaagaatt gataaaaaaa tatgattaat gggtaaattt aaaaaattgt gatatactag
     4381 gaacatatta aatatgagga tgcgtatttt atataaggtg ctttttagga ggtggaactt
     4441 tgaaacttca aaagatgggt atgaggacta taaaaacagg aattagtgtc actttatgta
     4501 tgattcttgg tgactattta gtagagaata tgttttattc tctggtagca tgtataattt
     4561 ctgttcaaga tacagtaaaa ggttctttaa agacgggaat caatagagtg gaaggtacta
     4621 ttttaggtgg tataatagga ttcatatttg caatcattaa agctggagac ccatttttat
     4681 gtggtctagg agtaatgtgc tcaatatatt tatgcaatct gtttaaaata aagtcagtag
     4741 tagttgcctg tgtaaccttt ctggctatac atctaggtgt aggaaatagt gaccctgtgt
     4801 attattcttt acatagggtg ttagatacat ctgtgggtgt tttgtttgga gtacttgtta
     4861 attattatgt tgctagacca aattatctaa atagtgttat agatgagttt aaaaagatag
     4921 aagaactttc aatgttgctt attgaagata aaattcttag aaaagaaaat ttaaatgtag
     4981 agaaatttga agaagaagta gaaaaattag aagaaatata ttcaaaactt attgatgagt
     5041 tggattatag tttgagtgaa actaatatag aaaaaattga gaatgcctta aggatatgta
     5101 gagaaataca ttttcatatg cagtctatag agttacttga aaataaactt tatttgaaag
     5161 agcatacata taaatacctt aagaaaacat ataatgtgga taagctagac tggaatatag
     5221 atgaagaaaa aagccctgta ttcaactatc atctaatgaa aataatgatt gaaatagagt
     5281 tgttgagaaa gctaaataaa tcatattaat aatagtagtt aaataataaa taaaaaatag
     5341 tatctttgta aatattaaac atatataagt ttaatttatg gaggtactat tttttatgaa
     5401 tactagaaat gacaattcta aaatactaga aatatataaa atgaagcttt ttggcgaact
     5461 tgtaaaagca gtgtggaatg atgatataga ggatattgaa aacttaccta ataaaatttt
     5521 aaatgaagat ttaaaggata atttaaataa ttattttagt ggaaaatttg ataaagatac
     5581 tatggtaaat ttaattaggg taactatggg attggatgtg acagaagata cagataaaaa
     5641 tttaaaagag tcactacatg aagctgtaaa tttagatgaa gtcaaaagac caataatatc
     5701 tgtaatatca gatgcttgca aatattgtga tgaaaataat aaggaagatg agtcatgtaa
     5761 gataagaagt aagcatatta attgtaatga ggaagatgta tgtagtgctt gtggtgattg
     5821 tgtatcaaga tgtaagttgg gtgcaatttc agataaaata gaatttatac caatgataaa
     5881 attgttaaaa gatataaaga gccctgtata tgcaatagtt gcaccagctt ttgtaggaca
     5941 gtttgggaaa gatgtaagcc ctggtaaatt aagaactgca ttaaaaaaga taggatttga
     6001 agatatgata gaagttgctt tggcagcaga tatgcttact gcaaaagaag catatgatta
     6061 ttatgagcat atgaaaaagg acgaagaagg atgctttata actagttgtt gctgtcctat
     6121 ttgggttagt ttgatacaaa atagttttcc tcagatattg gaaaatgtat caccatccgt
     6181 ttctcctatg attgcttgtg ggagagctgt aaaaatttta aatccagatg ctaaagttgt
     6241 attcataggt ccttgtatgg caaaaaagaa agaggcaatg ttagaagata taaaagatgc
     6301 agttgatttt gtgcttacat ttaaagagtt gcaagaaata tttgatgcat tagagttaaa
     6361 tccagcaata atggatgaga gtaatagagt agaggcgtcc ctttcaggta gagtttatgc
     6421 tagaacagga ggtgtaagta aagcagttaa gttatcagtt agaagaattg acaaaaatat
     6481 aaaatttgaa gaaaaggcat ttgaaggaac aaaagagtgc atggacggtc taaagaaagt
     6541 tctaaataaa gaaatagaag ctacatttat agaaggaatg gggtgtgtag gaggctgtgt
     6601 aggaggtccg aagcgtattt taagtgtgga agaaggtact aagaatgttg atgaatatag
     6661 tgaaagtacg aatatggaaa ctccatttga taatttaaat gtattacaat ttttaacttc
     6721 tttgggaatc aaaagagtag aatctttagg agaaaaagaa gaagaaaagg ttaaaaaaat
     6781 atttagtaga gatataaaaa gtaataagaa agagaataaa tataaaaaat aactttttaa
     6841 gtcttaaact tatgctttta taaaagggag atatggggga tgaaaattaa agataataag
     6901 gctgtttttc atgtatttat aaggatattt attatagctg ttttaatttt aactggaaca
     6961 ttattggagg aaaaaattaa taatgttcca gttgaaaata taagttcaaa aggtgaaaat
     7021 acactggaac tgctttctca aagtaatact ttaggagaaa tcatatacaa agcatctcaa
     7081 gaagaaacaa aaggtccagg ggctacttct gaaaaagtag catacataac aatagatgat
     7141 ggtccatcaa aatatacaaa tgcactactt gatattttaa aagaaaatga tgtcaaagcg
     7201 acatttttca tgcttaatca gaatatgaaa aaccacaagg aagaagtaag gagagtcttg
     7261 gaagaagaaa actcaatagg ttttcatagt gtaagtcatg atatacataa gttatataaa
     7321 agcccagaag taacagttgg ggaatttaat acatgcaata atactctaca agaaataact
     7381 ggtcagagtt caaaattaat aagattacca tacggaagta aaccatatat gccagaaggt
     7441 tcatataata aacttatggc aaatgattat ttaatttggg actggaattt ggatacacag
     7501 gattggaagt cttctacaag tcagatagta agtaatatat tatattatgg aagaaaaaga
     7561 gatgaattag tagtattaat tcatgagaaa gagcaaagtt taaatgcttt aaataatatt
     7621 ataagaatct taaaagagag aggatataca atattgccaa ttacagaaaa tataacacct
     7681 aaaaatttct ggcaagaaaa cttataataa atttaaatat agtaaaagat aaagtaaatt
     7741 atttataaaa taaaacatat aatataaaaa atacgaataa gaggtatact gtaaggataa
     7801 tataatataa aattaatgag aaagcattaa aattaaaata aaataatctt gcaattattg
     7861 acattataag acagaataac tgtaaaaaca tattttaaaa tataacaact atataaaaga
     7921 gaatagacat aattaagaaa attcagaaaa aataaaataa aaataattga caaagagaat
     7981 aatatttgta tataataata tgtaatatta aagactgaga agaagaggag taagtaaata
     8041 ctactaaaag agaggaaaat tcactggctg taagattttc taagtataag atttactgaa
     8101 tgtagctttg gagtttctaa actgaactat aagtaagttt agacggttag aaccgttaaa
     8161 ttctttgaga gtcaaagttg ataaaataac attaaccaat aatttaatta tatgttaatg
     8221 tattttaagc tttggaatta aggtggtaac gcgagctttt cgtccttttt aaagaggatg
     8281 aagagctctt ttttatttct aaagatattt aaaaaattta tttataaaat acaggaggca
     8341 ggacaatgga aaatacgaat ttatcaaaaa cttataaccc aaaagatttt gaggctaggc
     8401 tatataaaaa atggatggat gaagggtatt ttaaatctaa accaaatcca gataaaaaac
     8461 catttacaat aatgatgcca ccaccaaata taacagggca attgcatatg ggacacgctc
     8521 ttgaccatac acttcaagat atattaatca gatggaaacg tatggatgga tatgaagctt
     8581 tttggcttcc aggaactgac catgcttcta tagcaacaga agttaaagtt gttgaaagaa
     8641 taaaaaaaca agaaggcaag acaaagtatg aaataggtag agaagaattt ttaaagagag
     8701 cctgggaatg gaaagatgaa tttggaggaa aaatatctaa tcagcttaaa caactaggtg
     8761 attcttgtga ttgggataaa gaaagattta ctatggatga agggtgtaat gaagcagtta
     8821 tagaattttt tgtaagccta tatgaaaaag gtcacatata cagaggaaat agaataataa
     8881 actggtgccc agattgcaaa actactttat cagatgcaga agttgaacat gaagaacacg
     8941 acggaaactt ttatcatata aagtacccac taaaagatag tgaagacttt ttagaaatag
     9001 ctacaactag acctgagaca atgataggag atactggtat agcagtaaat ccagaagatg
     9061 atagatataa acatttaata ggaaaaacag caatacttcc attagtagga agagaacttc
     9121 caatagttgc agatagttat gttgatttag agtttggaac tggtgcagtt aagatgactc
     9181 cagcacatga ccctaatgat tttgaagtag gattaagaca taatcttgag cagttaaata
     9241 ctatgaatga agatggaact atgaatgaag tttgtggaaa atatgaaggt atggatagat
     9301 ttgagtgtag aaaagctatc gtagcagact taaaagaaca aggttaccta attaagataa
     9361 aagagcataa tcataatgta ggaacttgtt atagatgcca tacagtagtt gagcctagat
     9421 tatcagagca atggtttgtt aagatggaag aacttgcaaa accggctata gatattctta
     9481 aaaaaggaga attagaattt gttccagata aatttgataa aacttattta caatggctag
     9541 aaaatataag agattggtgt atatcaagac aattatggtg gggtcatcaa ataccagctt
     9601 attattgtca agagtgtgga gaaatagtag ttgctagaaa aatgccagat aagtgtccta
     9661 aatgtggaag cactcatttt aaacaagatg aagatgcttt agatacttgg ttcagttctg
     9721 cattatggcc tttctctact ctaggatggc caaataagac tgaagcttta gattattatt
     9781 atccaacaag tgtacttgtt acaggatacg atataatatt cttctgggta gttagaatgg
     9841 catttgcagg tatgttctgt atgaatgaaa agccatttga ccatgttttg gttcatggat
     9901 tagtaagaga ttctcaaggt agaaaaatga gtaaatcttt aggaaatggt atcgacccat
     9961 tggagataat cgagcaatat ggagctgatg ccctaagatt tacattaaca actggaaatt
    10021 ccccaggaaa tgatatgaga ttctatatgg aaagagtaga atttgctaga aactttgcaa
    10081 ataagttgtg gaatgcatca agatttgtat ttatgaatat tgatgaagat ataattaaaa
    10141 acatgactag agaaagtgtt aaagaggact taactctagc tgataaatgg ataatatcaa
    10201 gagcaaataa tattgtaaaa gaagcaacta ataacatgga taagtttgat ttaggaatag
    10261 cacttcaaaa gatatacgat tttacttggt cagaatactg tgactggtat attgaaatgg
    10321 ttaaacctag attatatgga gaagatgcaa atgctaaatc tgcagcatta tatacattaa
    10381 cttatgttct tgaaaaaata ttaaaattat tacatccata catgccattt ataacagagg
    10441 aaatatatac tcatcttcct actgttgaag gatgtataat agtatctgaa tggccaaaat
    10501 ataatgaaga agataacatg gctgaagaag aagatatgat gaacttattg atggaaggta
    10561 taagaagtat aagaaatgtg agagcagaaa tgaatgtacc accttctaag aaagctaaat
    10621 tgataataat cccaagtgaa gaaaaaatag aagctattga gcttggtaaa gactatttta
    10681 taactttagc atctgcttca aatgtagaga tagcaaaaga taaatctaat gttccagaag
    10741 atgcagttgg agtagttatt gatggtgttg aaatatttat acctcttaat gaattagttg
    10801 attttgaaaa agaaattgag agattatcta aagaaaagaa aaagttagaa ggcgaaataa
    10861 agagagttaa tggaaagctt gcaaatcaag gtttcttagc aaaagctcct gaaagtttaa
    10921 ttgaagaaga aaaagcaaaa aaagaaaaat ttgaagagat gattaaatct gttgaggaaa
    10981 gattaacaaa tttagaatct aaaataaaat aaatataaga attagtaaaa acatacaaaa
    11041 agtatatatt ctatactttt tgtatgtttt tctttatata tatacaaata tgatgttata
    11101 attctttaca gatgtgttat atttttattt tatgttgtga atatatgaat tttatgtctt
    11161 aaaatttata agcaataaaa atttgatata ttatgatgtt taatataggg taataaatac
    11221 atattatatt tattttacta tattttttta cataatatat ctagaaaagt ataggaattt
    11281 atcgtaattt aagtttaaat attaaagaga atttctataa gtagtttaat cgacaattta
    11341 aatttataaa gaaatttaaa tttgaaagct agtgagtaca gtttggaaat tgaaataaag
    11401 taaactaaat gatattttag atttggaggt gatattatgt tcagggtagt tatatgtgat
    11461 gacgaaaaaa tacaaagaag catactaaaa gagtttacag aaaaactttt gtctgaaaga
    11521 tgtctaaatt atcaaatact ggaattttcc tgtggagaag atttaatatc caaataccca
    11581 gaaaaaattg atattatatt tttagatatc caaatgaaag atatcaatgg aattgaggtg
    11641 gctaaaaaaa taagaaagtt tgatgaaaaa gtagaaatta tatttacaac ggctttttca
    11701 gagtatgccc cccaaggtta tgaggttcgt gcttatagat atttagtaaa gcctatagag
    11761 tatagtaact ttgcaatggg tgtaaatctt tgtattgata accttcaaag aaagaaggag
    11821 cgttatatag ttttaaatag taaaaaagga tttaatagaa tactgataga ttctatttta
    11881 tatgttgaaa ctgtaaagag agatttagtc gtacatacaa taggtagaga ttataaagct
    11941 aatatgagca tgaaacaagc agaaaaatta ctgtgtgaaa atggcttttt tagatgtcat
    12001 acaagtttta ttgttaattt aagaagaata gaagatctta gggataatat gattactatt
    12061 aatgaacaat ttatacctat aagtaagtat agacttaaag atttaaaagt agcattaaca
    12121 agcttgttat gtgatataat gtattaattt taaaaaggta attgaaaata tcgtaaaaaa
    12181 gatttaatgt catcacaggg ctattgattg aaactgtctt aatatataaa atgttattta
    12241 tattaagaca gttttattat gcaaaatagt agtttataga tttttaatat ggtttaacat
    12301 gtatatgtag agtttccatt ataatagcat gattattaat tagtagcttg attatcaata
    12361 tactattatt gatttttata tgcattatat gtatttttag aatatgatta cctttttaaa
    12421 ataatgacta tttataaaac tctatagata tttgtttcat ttagctacgc atactttaat
    12481 taattatatt aatatttaat ggagtacaat ataaatatta aagcgacatc ttatagttta
    12541 ttgaacctat agtaatgaat taataaatag tttaattcta tgaaaagatt tccttttatg
    12601 caatttattt attgaaatat gatataatat ataaaggtgt aagtattagt tgttaaaata
    12661 atatttatat tagtttttat acatacttgg tataagtttt tcatatattg attaaaattg
    12721 atgttgattt attattttta gtattttttc accttttgat tcatactttg tagaatggtg
    12781 tattttctaa aactgattat aagacaagtt cattcaaatt agcattttta tatcatataa
    12841 ataattgttc cccctaaata agatttagat gtatagtatt aaaatcaaaa agtataaaat
    12901 aaaattgatg cttaatcata gataaatatt aataaaatat acaaggggat tgtctaataa
    12961 aaagataatc tcctttttta caaataattc taaattgtga tataaatcta aaaattttat
    13021 cttaacattt aaccatttaa tgataataat tgatataata aatataaatt aaatatatgg
    13081 tgtacttatt ttatgttttt agcaagaaat atagcattta aaagataaat tgtgcaactt
    13141 aaatcaaagt ttaagatgaa ctattaacat agatatatta aaaacttttg taatggagaa
    13201 tatatgtgat atgaataata aagttaaggt tgcagtatta gatacaggaa tagacaaaga
    13261 acatgattac ttaaaagata atttagttgg aggaattgct tttgaatgta tgcatgatta
    13321 tatctttata tcagataaat ttgatgatga agatggtcat ggaactgctt gtgcatcaat
    13381 aataaagaaa gagtatgaag atgtagagtt atttgtaata aaaattttga gaaaagatgg
    13441 tattacaaat ataaaagttc ttgaagaagc attaagatac cttttagaca ctaacataag
    13501 attaattaat ttaagtttat cagtcatagg agtagaaagt gttaagggct tatttgaagt
    13561 ttgttatgaa ttgtttagaa aaggaaaaat aatagtatgt tcactcgcta atgattttga
    13621 cttaagttat ccagctatgt ttaacaatgt tataggtgta agaggattta ctttagatgg
    13681 agatgattca ttttggtaca acaaaaaata tgatgttcaa tgtgttatgg atagcaattc
    13741 atatataagt tgtgatataa ataattccta tagattacca ccaaaatgta atagttattt
    13801 atcagcaaag cttactggta aaatagctaa gatattatca gaaaaaccag gcataacaat
    13861 atctgattta aatgataagc ttgaatcatt agcaactaga aatcattggg atagttgtga
    13921 ttttgatgag tgtagtagaa ttgtaaactt taaattggat ttgtatgaca aagaaaatgc
    13981 tcttcttatg aaagttgctg acgttgttag ggattgctta gatattgaag ataataatga
    14041 aaaactcttt aagggtagcc tttttaataa ggagataggc ttggtttatg ataattgttt
    14101 taatctgttg aaaaagctag agcatagatt tgacataaag tttaattata tggacatctc
    14161 aaaatatgac ttagtttcaa tatatacact gactgaatta gtggaaagat ataaaaactt
    14221 ataaataaag atttgtagct aaatttaatg tagaaggtat taaattatat gtattctttg
    14281 tcggtagatt aaaagtaaaa aatatactat aattagatag cataagagaa aatgccaata
    14341 cagggggagt tagataatga aatatgaaga agcattagag tacatatcac aaactaataa
    14401 attcggtata agattaggat tagaaaatat cggaaagtta ttagaattat taggaaatcc
    14461 acaagaaact ttgaatataa tacatgtagc tggaactaat ggaaaaggtt ctgtgtgttc
    14521 ttttgtttct aatatactaa gagagtgtgg atataaagtt ggtttatata catctccata
    14581 tttagaaact tttactgagc gtataagagt aaatggacaa aacataccac aggatgatgt
    14641 tgctagaata attgagttaa taaaagaaaa aatagagatt atggtaaaag aaggctatgc
    14701 atatccaaca gaatttgaag tggttactgc aatggcattt tattactatt cagaacaaaa
    14761 ggtagacttt gtggcattag aagttggttt aggtggcaga tatgatgcta caaatataat
    14821 aacaaaatca cttgtaagtg tgatagtatc tataagtttg gaccatacag gtatacttgg
    14881 agatacaata gaaaaaatag cgtacgaaaa ggctggaata ataaaggaaa atggagtagt
    14941 actagtatat gaccagactg atgaagcaaa ggatgttata aagtcagttt gtaaagagaa
    15001 aaaagctaaa tatatagaag ttgattttga tgatataaac ataaaaaaat cagatataaa
    15061 ttctcaaata tacgattgta ctgtaatgaa agaaacttat agagaccttg aaataaaatt
    15121 gataggcgaa catcaaataa acaattctat cttggcaatt agtgtaataa aatatctaaa
    15181 agacataaat aaattagcaa atataaatga agaatctata agaaaaggac ttataaatac
    15241 taaatggcca ggaagaatag aaaagataaa ggaaaatcca atttttataa tagatggtgc
    15301 acataatgaa gatggagcaa aatctttggc aaaagcactt gataagaatt ttaaaggtag
    15361 aaaattaacc ttattaatag gtatgttaga agataaagat attgatagtg ttttagagat
    15421 attgttgtca cattttaata aggtaataac aacaacacca aataacccaa gggccataaa
    15481 ttcagatata ttaagagaaa aagtattaaa atatgtagat gatgtaacat caaagcatga
    15541 gatagaggat gcagtaaatt atacattaga aacatcaagt gaggatgata ttataataag
    15601 tgcaggttca ttatatatga ttggtacagt gagaacttta gtaaagaaat tataatttta
    15661 taaaataagt atacaaataa aaatataaaa aagagttgtc taaaaaatgg acaactcttt
    15721 ttatataaaa taagttttta ataattttac tgatgtttat ataagtaatt atatagattg
    15781 acgtatagct ttagataatt ggtctgggca agaagtacct tttccaccac agtcaatacc
    15841 atttaattta tcagcaactt caagagcatt ttggccaatt attaagcttc taagtcctga
    15901 taaattacca ttacatcctc ctataaactc agcatcaaca atcacattat tttcatcaac
    15961 ttcaaaaatc atttctctac aacatacacc acttggatta aatgttactt tcattaaaaa
    16021 gacctccatt aaatattatt tcttttttta acattaataa taataccaaa tatatgcgta
    16081 ataatgcaac tataaagcat aatacaagct aatgaaataa tatattattg tagtatatgt
    16141 attgtagaga ggaggggtta tatgaaaatc tatatttcta actacttatc aaagagtatt
    16201 agcatagttg attattctac acttgaacta gaaaaagaaa tagtattaga agataatata
    16261 tatccacacc atttttgcat agaaaaagaa aaaaatttaa tatacatacc tagttcaagt
    16321 aatgggatac tctatgtctt agatttaagt aatgataaaa taattgatac tgtctctata
    16381 ggaggaagtc ttagtcaagt tgtacttagc gataatgaat tgtttgtggc aaatgaagat
    16441 tctaatagta tatatgtact agataaaaac acattaaatc caataggaat tattggagtt
    16501 gataatatgc cacatggatt tgactttgat agagaaagca aaaagttata tgtaccgtgt
    16561 ataaactcga tagtatgtat agatacaatt aataaaaata tacaaaagaa aataaatatg
    16621 gactttagag cttggcatat agaacttgat aaacaaaaga aagaaatgta tatatcaaca
    16681 cttgatggaa agcttataat aatagatgaa gtaagcatgg atataaaaaa ggtattatat
    16741 gaatttttat taccagtaga aataagattt aattatagtg gcaaaaaagt ttatgttgct
    16801 gatttgggtt ataataatgt aagaatttta gattatacaa cgagtagata cattggaaat
    16861 attgaaataa atgggattcc ccaaggatta gaaatatcta aagatgaaaa gttactattt
    16921 gtatcagata cacaagaaaa ttcagtaaaa gtttatgaaa ctgcaaataa caaattaata
    16981 aaagaaatca aggtaggaaa agagcctaca actataattt gtttgtaggc tttaatgtat
    17041 gtctcttata agtaaatcga taacttcggc atacatataa gatgcattat ttttccagtt
    17101 attgcaaata agttttgctt gtttctctga tgaaacattt agatttaaat ctattaagtt
    17161 agactgattt tctatgactc taaggttgac aataaattca ttatcacttt gttttacaaa
    17221 actagacttt acttgagtat ctgctagtag attttctttg ttagaatcta tatacttgtc
    17281 tattttttca gtaacactag taggtattcg attaaagaag tattctagac tttctacgcc
    17341 tctttgagta agagcataat attctctgtc agagtcttta tatgtagtca aaaacttaga
    17401 ttccataagc tgagataaaa gctgttgtaa tgaaaaataa ttcatcatct cagtctctaa
    17461 tactacttga gttatttgag aattagttaa atccatttta attttttcta atatatataa
    17521 aattaaaagt ttgtgatagg ctaattcttc agacgagttt tcaaacataa atatcatcct
    17581 ccgtcaaact atttctatat ataatatatt aaactaattt gtacaaaatt aaaagaacaa
    17641 tttgtattgt attattaatc tttaataatt catataaaaa caattttata aataaatata
    17701 gagaaacagg gctgctaatt agcagccctg ttgataccca ggtgtcaaaa tttactattt
    17761 attttccagc catttgtctt tcagccattt caactaattt cttagtcata tatccaccaa
    17821 catatccatt ttctctagct gttaagttac ctttgtctat gttttcatag ttagataaac
    17881 ctatttcgtt agctatttct aatttcattt ggtttaaagc agcttttgct tctggaacta
    17941 ctgttctgtt tgaatttgac atagtaaatt tcctcctcaa ataattagtt tatgatgtct
    18001 aacaacactt ttatttatgt gatgttaata ctaatttctc caaaataaaa ataatatata
    18061 cagaaaaata aaggtaaaaa tgtaaaaaat ataaaaaaat taaataaatg agtattttag
    18121 agagtttaat taggtcaata taggttttat ttttttagtt attgtcttat aaattaaata
    18181 ttacagtact tttttgagct gaaaaaaaat aaattttaaa tatagaagaa attatttcaa
    18241 agggggaata gtatgaaaag agaagttaaa aggactaaag ctttgatata tagtgtttta
    18301 tttttttcat tattattagt gttaatattt gtttttgttt atggaataaa atacatatgt
    18361 tctaaatctg acaattcaaa aatgcctata tataaagttg atacaaatga gaaaaaaata
    18421 gctttgagtt ttgatgtagc atggggaact gataatatgg atgatatttt aaaaatttta
    18481 gataaacata atgtaaaagc taccttcttt ttagttggta gttgggtgga tgataatgaa
    18541 gaaattgtaa aagctataga tgaaaaagga catgaaattg gaaatcattc aaatactcat
    18601 gccaatttga aggaaatatc aaaagaagac ataacaaaag agatagaaac tacatcagaa
    18661 aaaatatata atataacagg aaaaaggact aatttattta gacctccttt tggggacgta
    18721 aacaataaag ctatggacat ttgtagtgat ttaggttata aagtaattaa gtgggatgta
    18781 gattctatag attggaagga gcttggacca aatcatgtaa ttgagaaagt aataaaagaa
    18841 tctcaacctg gttcaatagt tttattccat gctaatataa atgatgtaga taactatttg
    18901 gatactataa taagtcgact aaaaaaagat ggatacagtc tagtcaaaat atctgatttg
    18961 ttgtataaag ataattatgt agttgattcc aatggagtgc aaaaaactaa gaattaataa
    19021 gtaataaatg tatattttaa gtaaagttgt gataaattat atagtatatg ttagaaaagt
    19081 gtcgataaaa agacggtgtc tattttttga cactttttat acatgtatgc aatatcaata
    19141 attatagatt aaattgatta aaaaagtgtc taataattga cttttttttg atgggtttaa
    19201 aaatttagaa gaattaatga atgctgtaag acagtatttc caaagggtat aagtttgttt
    19261 aaaagttggc atagaaattg ctttgttaat aagcgatatg ttctatagaa ataaacaata
    19321 tgttaagacc ttataataga aaaatagcat taacattatt tgaacagctt ataaaaaaat
    19381 taatatactt aagggggcgt actagttgaa caaactatat tctattttat taaaacaaca
    19441 cgttggtggt ccagataagc cagtagtaag tgttggagat gtagtaaaaa aaggaactct
    19501 aatcgcagaa ccaactggat taggggcaaa tatttatgcc agtgttagtg gaaaaataag
    19561 tgaaataaat gaccaagcga tagtaattga agctgatgaa gtacaagaag atacttttga
    19621 accattaaaa ggagaaggta tacttgactt aattaaagaa gctggagtag taggaatggg
    19681 tggagcagga ttccctactc atgtaaaatt aaacatagat ttaaatggtg gaacaatatt
    19741 agcaaatgca gctgaatgtg aacctttatt agctcataat ataaaagaaa tagaagaaag
    19801 accagaaata gtttatcaag gtataaaata tgctatggaa gttactaatg ctggaaaagg
    19861 tatgctagca ataaaaagta agcatccaaa agcaatagaa gctttcaaaa aagtaataaa
    19921 gccaggagac aacatagaag ttgcagaatt agttgatatg tacccaatgg gagaagagag
    19981 agcaatagta agagacgttc taggaaaatt acttgagcca actcaattac cttcagaggc
    20041 taatgcagta gtaataaacg tagaaacact aactagaata gtagaggctg ttgagcaaaa
    20101 aagaccagtt atatctaaaa atataactgt agttggtcaa ttaaacagtg gaaaagaatc
    20161 tatagtgttt gaagatgtgc caattggaac aactgtcgga gaattaattg aaagagctgg
    20221 tggaataaaa ggtgaatatg gtgaaataat cctaggagga cctttcactg gtaaagctac
    20281 tactttagat gctccaataa ctaaaactag tggtggtata atagttacta tgccatttgt
    20341 taatgaaaag agaaaaatgg gtcttttagt ttgtgcatgt ggacctaatg aagaaagaat
    20401 gagagatata gcaactaaaa tgggagttac tgatatagta tcagttcaaa aatgtaaaca
    20461 agctcaagaa attaaaggtg cattaaaatg tgaaaatcca ggacactgtc caggacaagc
    20521 tcaaaaatgt attgaattta agaaagctgg agcagaagtt atattaatag gaaactgtac
    20581 tgactgtagt aacacagtaa tgggttctgc accaaaatta aaattaggtg tatatcatat
    20641 tactgaccat gtaatgagaa cagttaatca cccattaata agaagaataa aaggtgacgt
    20701 aagattaaaa ataaaataat agataaatag aagttagaca tctttagaag taaaacttaa
    20761 aagttacttc taaggatgtt ttttaatgta ttttgagggc aatatatata ttgtaaaata
    20821 aatggaaaaa atagacaaaa tatgctagat attgtataat gataacaata gctattacaa
    20881 ttaatattgg acaaaaataa tgatactcaa gtatgtggtt tgtattaata tgcatattat
    20941 attaagtcaa ctatccgata gagtaaaagg tttttgtgat atttgaagat actctatagt
    21001 atctttttta ttataaaaac taatttttgt tttttattag ataagtatct aggatactaa
    21061 tttatatttt attgagttgt taaaaaagtg cacttaaaaa gttaaaaaag aaaaattaaa
    21121 aacctattta tagaaactgt gaagtatatc ttaaacctgg gcacttaaaa gatatatgga
    21181 gttagtagtg caacctgcta taaatataaa taggagtaac ttttaattgt caaaaatata
    21241 taaaataaat acataaataa gtaattgata gaagatagat ataaatatat ttttgaaatg
    21301 ctccctgcat tcataacgag tgtagagtgg cagatatctg cccaaaactc ctttaattgc
    21361 tggaagttcc taaagctaac taaaccacaa cataatcatg aaataaggat aagtgtgata
    21421 gagtgtgcat ttaataagca cttgaggtta cgaaagtaga aaaaattagt tagatagcat
    21481 aaggttaaat cctaagtgcc ttgaaaatgg ataatcagca ggtaagcttc gaaaagaaga
    21541 aacttcaacg actagagaga aatctcgtac ctacaagtgt ggggaaatgg ggagtaccta
    21601 atttatggtt aggtaatgag atagtctgtg cttaatagaa atattaagaa gttcatagga
    21661 gaactggtta ggagtaacga ccctaattga acacactaaa gatgtattat tgaataagat
    21721 tcatataata tttaataatt ttaattatat taagtgaata caatatatgt ttttagtatc
    21781 aggagaattt tgaaaacaaa aattaaaaaa tcaagtataa tcagtttagt aattgctttt
    21841 agtatggtat ttactgcatt tacaccaata gtatcttatg ctgacgaagt gactagcaat
    21901 gatacaatac ttaatggaga agaacaatca aatggtcaga caccagatgt agagaaacca
    21961 tcagacggtc aggtaccaga tggggagaaa ccatcagaca gtcaagtacc agatggggag
    22021 aaaccatcag acggtcaggt accagatggg gagaaaccat cagatggtca gataccagat
    22081 ggagagaaac catcagatgg tcagatacca gatggagaga aaccatcaga tggtcagata
    22141 ccagatggag agaagccatc agatagtcag acgccagatg tggagaaacc atcagatggt
    22201 cagatgccag atgtggagaa accattagat gagcaaactc cagaagaaga aaaaccattg
    22261 gaagaaaaaa tagttataga ggaaatgagt ttaaaacaag atattgataa aattcttgat
    22321 atgactttat ctcaaataaa taaaatagta tataactttt gggaagatga agaagatgta
    22381 aaggcagatg aacaatcaga aattaaccaa gtttttactt caaaagactc atttattagc
    22441 ttatggtatg acaaaaaagc aaaagtaaaa aattcttgtt tactaaaaga aatagatggt
    22501 tcagatagac cttatcatga tttaagattt gatgatatta caaagacttt aacttttgac
    22561 tatgtaataa agggtcttgt aggagcaagc tcaaaagacg acaaatatgt aatagaaggt
    22621 gaagatggag agcaaactgc attttgttac aataatcatt taagaccacc aagtagtaat
    22681 ggaaagagtc catatttacc agcagaagat tttaatggtc aagaaaacca aaatgaagaa
    22741 gcagttaaat ctatactata tgcaggttct gaatttgatg gatttggata taagcaacaa
    22801 tttaatttag gtggagaaga aaatgaattg atgacttact cagctactca aagtgcaatt
    22861 tggattattc ttggacaaat ggatgaacaa gaaagactta agcagtatca aggttctata
    22921 aacctttgtg ataaacttat agaaagagca aaaactgaag aggaaagagc ggagtatcag
    22981 aagaggaaag aagtagcgat aaatacaaaa gcttatttag aagcattgtt aaaagcaggt
    23041 cgtgaagagt taaagccaaa tgatacagga aaacctagtt tgagtaatgg tttaactaag
    23101 attaactttg aaaaaaatga agatggtact tatgaaacag aagctgtagc cttagtaggt
    23161 tatagtggtg tagtaaagtt aaaactacca gatggagtaa ctgcttatga tgtagatggt
    23221 aatataatag gtacaggcga agtagaaatt tcaacgcaac agaaatttaa acttaagagt
    23281 gttggaaaac cagatgccaa agctaatatt tctgctgtat cttatgatta tatttttcca
    23341 aaagctattc aatattataa ggcagtgctt gatttaggac aaaaagacca ttcatcttca
    23401 ttaccatcta gcaagcaaaa tttattaagt tatacaatag agaaaaagaa tggtcaagaa
    23461 gttaacttta atataggttt accaacagat gatgatgttg taaatccacc agtgccacca
    23521 atagatgatg atgttgtaaa tccaccggtg ccaccaacag atgatacaaa tggacataag
    23581 ccaaaaccat caccaccaat agatgataca gtaataaatc caccagtacc accaatggat
    23641 gatacaataa taaatccacc agtaccacca acggaggatg cagtgttaaa tccaccagtg
    23701 ccaccaatgg aggatgcagt gctaaatcca ccagtgccac caacagatga tacagtgtta
    23761 aatccaccag taccatcagt aagtgataca gtggaaaaaa ctccagaact atcaagagat
    23821 gatacaataa taaaatcacc aaaaacaggt gatgaaactc agctaatgtc atatgtattt
    23881 attagtgtca ttgcaatttg tggattagcg tatcaatgta aaataaaaag aaattaatct
    23941 agattattat taagtttaaa ataaaaagtc tcaaattgat ttataagtca ttttggggct
    24001 tttttatgta aatataatat gcaaatatag tttgtttaaa aatatctatc ttaattatat
    24061 tcttaaattt atgattggat acaggcattc attaattgtt tgatgaatag aaaaatgttt
    24121 tgaaggtgaa aatag
//
LOCUS       DAGSWI010000055        23772 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.055, whole genome shotgun sequence.
ACCESSION   DAGSWI010000055 DAGSWI010000000
VERSION     DAGSWI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.055"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            15..1094
                     /gene="mltG"
                     /locus_tag="MJF80_002008"
     CDS             15..1094
                     /gene="mltG"
                     /locus_tag="MJF80_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBZ0280669.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            1266..1940
                     /locus_tag="MJF80_002009"
     CDS             1266..1940
                     /locus_tag="MJF80_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBZ0280670.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            1927..3174
                     /locus_tag="MJF80_002010"
     CDS             1927..3174
                     /locus_tag="MJF80_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBZ0280671.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            3246..4910
                     /locus_tag="MJF80_002011"
     CDS             3246..4910
                     /locus_tag="MJF80_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBZ0280672.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(5007..5648)
                     /gene="sigK"
                     /locus_tag="MJF80_002012"
     CDS             complement(5007..5648)
                     /gene="sigK"
                     /locus_tag="MJF80_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HBZ0280673.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTP
                     REREIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            6215..6577
                     /locus_tag="MJF80_002013"
     CDS             6215..6577
                     /locus_tag="MJF80_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280674.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            6561..7277
                     /locus_tag="MJF80_002014"
     CDS             6561..7277
                     /locus_tag="MJF80_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280675.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEYEEELKQNIIE
                     NIKKNFDEKNLVQVYKYRGV"
     gene            7297..7716
                     /locus_tag="MJF80_002015"
     CDS             7297..7716
                     /locus_tag="MJF80_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280676.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            7706..8230
                     /locus_tag="MJF80_002016"
     CDS             7706..8230
                     /locus_tag="MJF80_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280677.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            8542..9099
                     /gene="efp"
                     /locus_tag="MJF80_002017"
     CDS             8542..9099
                     /gene="efp"
                     /locus_tag="MJF80_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBZ0280678.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            9191..9646
                     /locus_tag="MJF80_002018"
     CDS             9191..9646
                     /locus_tag="MJF80_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBZ0280679.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            9809..10519
                     /locus_tag="MJF80_002019"
     CDS             9809..10519
                     /locus_tag="MJF80_002019"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBZ0280680.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            10573..11643
                     /locus_tag="MJF80_002020"
     CDS             10573..11643
                     /locus_tag="MJF80_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBZ0280681.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            11671..15225
                     /gene="smc"
                     /locus_tag="MJF80_002021"
     CDS             11671..15225
                     /gene="smc"
                     /locus_tag="MJF80_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBZ0280682.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            15252..16532
                     /gene="ftsY"
                     /locus_tag="MJF80_002022"
     CDS             15252..16532
                     /gene="ftsY"
                     /locus_tag="MJF80_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBZ0280683.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMNSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            16635..17003
                     /locus_tag="MJF80_002023"
     CDS             16635..17003
                     /locus_tag="MJF80_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBZ0280684.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            17000..18352
                     /gene="ffh"
                     /locus_tag="MJF80_002024"
     CDS             17000..18352
                     /gene="ffh"
                     /locus_tag="MJF80_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBZ0280685.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            18390..18662
                     /gene="rpsP"
                     /locus_tag="MJF80_002025"
     CDS             18390..18662
                     /gene="rpsP"
                     /locus_tag="MJF80_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBZ0280686.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            18727..18954
                     /locus_tag="MJF80_002026"
     CDS             18727..18954
                     /locus_tag="MJF80_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBZ0280687.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            19062..19577
                     /gene="rimM"
                     /locus_tag="MJF80_002027"
     CDS             19062..19577
                     /gene="rimM"
                     /locus_tag="MJF80_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBZ0280688.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            19586..20281
                     /gene="trmD"
                     /locus_tag="MJF80_002028"
     CDS             19586..20281
                     /gene="trmD"
                     /locus_tag="MJF80_002028"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBZ0280689.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            20466..20816
                     /gene="rplS"
                     /locus_tag="MJF80_002029"
     CDS             20466..20816
                     /gene="rplS"
                     /locus_tag="MJF80_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBZ0280690.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            21137..22033
                     /gene="ylqF"
                     /locus_tag="MJF80_002030"
     CDS             21137..22033
                     /gene="ylqF"
                     /locus_tag="MJF80_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBZ0280691.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            22422..23714
                     /gene="brnQ"
                     /locus_tag="MJF80_002031"
     CDS             22422..23714
                     /gene="brnQ"
                     /locus_tag="MJF80_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBZ0280692.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT        10010 a         2295 c         4364 g         7103 t
ORIGIN      
        1 gagtagatta tattatgaac tttaaagaaa atagattgaa aatagcagta ttaataattg
       61 ttattttgat tatcttagca ggtatatttg tgtttataca aataggtcct tatgataaaa
      121 ataacaaaaa agatgtaatt atcgacgttc caagtggagc atctgttgga aaaatatctg
      181 acatattata tgaaaataaa ttaataaaga atgaactatt atttaaactt ttggtaaagg
      241 ttagcaataa agctccgtct ataaaatcag gaacatactt acttaatcaa tcgtattcaa
      301 acaatgatat aataagtctt ttagtttcag gtaaaatata tcaggatggt ataaaagtga
      361 ctattccaga gggagctaca tctaaagaaa ttatagcaat gttagtaagt aaaaatttag
      421 gagataaagc tacttttgaa aatttaataa aaaaacctca agaattttat gataaatttc
      481 catatttgaa ggaagatgga ataacctctt tagaagggtt cttgtatcca gaaacttatt
      541 attttaattc aaagaaacaa tcagaagaag atattttaag cgaaatgtta aaagtatttg
      601 acagtaaata cacggacaag tttaaaaaga aacaaaaaga attaaatatg actcttcaag
      661 aagtaatgga aatggcatca ataattgaga aagaagctgt tcttgacaaa gacagaccta
      721 taatagcaag tgtgttttat aatagactta aggtaggtat gcctcttcaa tcagatgcta
      781 caatacaata tatatttgaa gagagaaaga aaattgtaac atatgatgat ttaaaaattg
      841 attcaccata taatagttat aaaaataagg gattacctcc tacaccaata tcaaatcctg
      901 gtataaaatc tattgaagca gcattatatc cagagaaaac agactattta tattttgtag
      961 ctaagattga tggtggaaat aattatagta caaactatca agaccattta aaatacgtta
     1021 aagaatataa agaagcaagg gataaacaat caaaagatac aaaagcaaca aataaagaaa
     1081 atacaaaaaa ataaaccatg attataaaaa gtctaacagg gtaagaataa tgttgggctt
     1141 ttttattgaa aattgttatt cttagattta taataaagaa agtgtaaata tatataacaa
     1201 tatgaattta aaaaataata aattaatata aaacttgaat atttattatt ttagggagtg
     1261 tttttatgag taatatagtt aatgatttgg ttgaagatta tataagagtg acattaaaag
     1321 aaaaagaagg atttttaaaa gatttagaaa tttatgcaga ggaaaatagt gttcctataa
     1381 tacataaaga agtttcagat ttattaaaag tcttgttaaa ggttcaaaaa ccaaagagaa
     1441 ttttagaagt aggatgtgca attggttatt cttctatttt ttttgcaact attataggaa
     1501 aagatgcaga tataataact gttgagagaa atgaaaaaat gatagaaaaa gcaaaagaaa
     1561 acataaaatt agctggattt gacaataaca taactatact agaaggagat gcagaagaaa
     1621 aacttagtca agtccaaggt gaatttgacc ttatatttat agatgcagcc aaaggtcaat
     1681 ataagctatt ttttgattta gttatagata aattaaaaga tgggggactt ttagtatctg
     1741 ataatatttt gtataaaggt atggttgctc atgatgattt tgttgtaaga agaaaaaaga
     1801 ctatagttaa aagaatgaga aattatttag attatatatg taattgtgac tatttaagca
     1861 catcattggt accaataggt gatggtgtag cactaagtta taaagaaagt aaaagaggtg
     1921 atgtagatgg aattaaataa agtagaactt ttagctccag ctggtgattt ggagagattg
     1981 aaaatagcta taacatatgg agcagatgcc gtttatatag gtggagaaat ttttggaatg
     2041 aggtctgcag cgaagaattt tagtaaggaa gatatggcag agggagtagc ttttgcacat
     2101 gaaagaggca agaaagtatt cgtaacagta aatataatac ctcataatga agattttcta
     2161 caattagaag actatttgtt ggaactagaa gagataggaa tagatgcagt tataatagct
     2221 gacccaggag tgcttagtgt cattaaaaaa gtaattccga atatggaaat acatttaagt
     2281 acacaagcaa atacaaccaa ttatctaagt gcaaacttct ggtacgagca tggaataaaa
     2341 agagttgtag tggcaagaga attatctttt gatgaaatat ctgagataag agcaaaaaca
     2401 cctttagata tggatataga agcgtttatg catggtgcta tgtgtatttc ttattctggc
     2461 agatgtttaa taagcaacta tatgactgga agagatgcaa acaaaggttc ttgtgctcaa
     2521 tcttgtagat ggcaatatca tttggtggaa gaaaaaagac ctggtgagta ttttccaata
     2581 tacgaagatg aaaggggtac attcttcttt aactcaaaag atttatgtat gatagagtat
     2641 ataccagagt taatcaaatc tggtataaca agtttaaaaa tagaaggtag aatgaagact
     2701 gcatactatg tagctactgt tgtaagagca tacagaatgg caatagatga attttacaga
     2761 gacccagaaa actggaagtt caatccaatg tggatggaag aactaaaaaa aggtagtcat
     2821 agacatttta catcaggttt ttacttgaat aaaccaacaa cagaagacca aaattatcaa
     2881 tcagcatcat atgttagaaa ctatgatttt ataggtatag ttagagaaac agaagatgaa
     2941 gatggtctta ttgttgttga gcaaagaaat aaaatgtgtg ttggtgatga aatagaagtt
     3001 atgggaccat ataaagaaac tatgtttact aaaattgaag aaatgtacaa tgaagaagga
     3061 gaagctatag aatcagctcc tcatcctaga caaatagtga agttaaagct atcagtaaaa
     3121 gttggtaaag attatatgct tagaaaagtt atagaagaaa aggttgaaga ataataaaaa
     3181 attgataaaa atagtattag ccccttagaa attgtcaata atgaaattat ctaaggggtg
     3241 atttcatgtc aaaaaagaag actccatttt tgaaaaaagt gggaaagaga agctggtgta
     3301 tatttactat aattttaata atttatagtg ttttaattta taggctagtt gatatacaag
     3361 tattaaaagg tgataaatac aaacaaagtg ttgaatcaca aagcgttgag aaagtcgagt
     3421 taaatagtgg aagaggaata atctatgata gaaacaacaa gaagcttaca gatacaagta
     3481 aatctcaggt attaattgtt gaaaaagaaa aattaaataa taactataaa attttggagc
     3541 ttattaagaa agctactaag atgaatgatt tagatatata taaagctgta caagagcaat
     3601 tgacacgccc tataatacag attcagacta aaaatatcga taagagtatg aaaaaagaac
     3661 ttgagaaaaa tggaataatg gtagaagaaa aaactatgag gtacgcaaaa gatggtttat
     3721 tatcacatac aataggatat ataaaagaag atgataaatc aggtcaatca ggaattgaaa
     3781 agagtatgga tagtgtactt agaaattcaa atgaaaagta tataagtgct tttaaagctg
     3841 gagatgcagg aaatgaaaaa tctctaaaca tactaaaggg aagtgtaaaa actgttgata
     3901 ataaggataa agatagacat ctaaaaacta ctattgatta taacattcaa aaaaaattag
     3961 aacaaatact aaataaagaa gaaaatccta cagcagcaat aatctcggaa gcatctacag
     4021 gtgaaattct tgcaatgtgc tctagaccta attttgacca aaatgacata tcaaaaagtt
     4081 taaaaggaaa aaatggagaa tttgaaaata gagttataaa agcgacatat ccaccagggt
     4141 ctgtatttaa gatggtagtt ttattttctg cacttgaaaa tggtgttata gatgagaact
     4201 atacttacaa ctgtacaggt aagactaaag ttgggaatac taatgaaata ctgcgatgta
     4261 ataaaagaga tggtcatggt ttccaaaact taagacaagc tttttctaat tcatgcaatc
     4321 ctgcattttt agatatagct atgaaacttg gaaaagaaaa gatactcaaa tctgctgaaa
     4381 aattacattt atttgaaaag gttgatattg gtcttgatga agaaaaaatt agagaagctc
     4441 caaaaaacat atcaataaga aatttagcta taggtcaaga aaatatagaa tttacaccac
     4501 ttcaaataaa tcaaatgact caaataatag ctaataatgg aacattcaaa ccactatact
     4561 tatataaaag tcttgtggac aataatatga atacaataaa aacttataag tcttcaaaaa
     4621 aagaagaact tatttctcct tatgtgtgta cacaagttaa agaatatatg aagtctgtat
     4681 ctaggattgg tacagctaaa gacctaaaag atattgaagg tggttgtgga gttaaaactg
     4741 gtacagctca aagtagttta aataaaaaag ctatagacca tggatggata acaggatttt
     4801 atcctgaaga aagacctaag tatgtaataa ctgtgttagt agaaggaact caaaaaggaa
     4861 ataaatctgc aacacctata tttaaggaaa tatgtgaaag tataaaataa attatgtact
     4921 ataaaattta aggatatatc gtaactttga ttttagatag aaaattttgt ctaatgatgt
     4981 acgatatatc cttttaaaat atagtattaa ctttcttgaa caagctcttt ttctagtttt
     5041 ttaagagctt ttttttcgat tctagataca tatgaccttg atatatcgag tttttgtgca
     5101 atttctcttt gtgtcttata gccataagga gttaacccat atcttaactg gacaatttct
     5161 ctttctctag gagtaagaat tttgtttagt tgttcgtaaa gttttccaac ttgaactttt
     5221 agttcaactt catcaagaac atagttagcc tctgtaccaa gtatatctaa aagacttatt
     5281 tcattacctt ctttatctgt tccgataggg tcttgtagag atacttgagt tttatttttt
     5341 ttgttagatc taatattcat taaaatctca ttttcaatac atctagaagc atatgttgct
     5401 aatcgagttc ctttatctat gttataagtt tctatagctt taattaatcc tattgtacct
     5461 atggatatta aatcatcctg atcttccgtt gaattattat atttctttgc tatataagct
     5521 actaatctca tgtttctttc aattaatgta tcttttgcac ttttatcgtt ttctatttta
     5581 aatttagtta aatactcaat ctcttcttca ggggttaagg gtttttcaaa agatttaaga
     5641 gctgccaata ggtcaccccc ttttcaacgg tcactttaaa ttatatgaag gagacttatc
     5701 caagttgact gttatctaat aaaataatat gcatattttt agaatttaaa acatatattt
     5761 gcaaactttt gaatggaata tgacataaag agtgggaatt taaatttaag tttttgttgt
     5821 atatatatga aataaggcga agaaattatt gatttataaa gtataaattg gaattgaggt
     5881 gtcaataaat ataaaaggaa atagtattta tatatgtttt ggtgttgaag taaaagatta
     5941 atgcaaagca ttagagtcta taaaaatatg aatataggag gtgtaatttt acaatactta
     6001 aaaaagtaaa tgtaattaat tgtttgtata aagttgatta caagcaaaag tatcagtcat
     6061 agttaatact gttaagggtg aaggtggtgt gttaatttat atatgtatgg gaagaaattt
     6121 tgattacaat gaaaaaactt acaaaagata tttatgaaaa aacttatagt taaatgttta
     6181 tatataaaat attatttagg ggttgttttt attattgaaa ataagaaaga gtggttttat
     6241 atcaattgaa tgtataataa gtattgctat attatatgtg gctgtttatt tagtttctac
     6301 atcattgtat aattgttata gttttatcag tagaaatata tctgacagag aaatgttaag
     6361 tacagcaaaa aaatatatag aagatgagaa gtatagaata caaaatagta agtatgagtt
     6421 aattgaagat aagatagaaa aaaattacat aaatggatat gaaattaaca gtaaaataga
     6481 gcaaatttta gattattatc aatgctatga aataaatata gagataaaaa atgaatttaa
     6541 aaaactgagg tttaatagct atgttactag aaaataaagg ctctatatta ataataacat
     6601 tgataatatt ttctattata agtactgtgt gcattatgtg tataggttta atttatacac
     6661 ataataatat gtttgagtta ggatacaagg acattcaact aaaagaaatt tctttaagtg
     6721 gaatagaaat atcagtaagt aatgtacttt cttgtgttga ggaagctatt gatgtatcta
     6781 ataatgaaga cgaatttaga gaatatttct taggaaataa tatgattgaa tgtataaaaa
     6841 aaataaaaga tacatcttat agtagtttag aagaactttc tttaaaagta gaaaataata
     6901 ctatttatga cagaggagat ttttttgaat ttgatataga aagtaatgtt agagaaaaag
     6961 aatttttaaa agcaataaaa gtaagagtga aaatcaaaaa tccatggata ggaattgaca
     7021 ttgaagatta tatggataaa tctaataaca ataaaattga tgacaaaaca ttcaataatt
     7081 ataaagcatc tgaaaataat gaaattctaa atcaatttaa aaatgaaagt gaaaatataa
     7141 ggaatcaaga ggttggtgga tttacagaag aagatgaaga atatgaagaa gaacttaaac
     7201 aaaacataat tgaaaatata aagaagaatt ttgatgaaaa aaacctagta caggtatata
     7261 agtatagggg tgtataactt tgaaaaaaga aggaatgtga ctgtaataga attaatagta
     7321 tctatcagta taatactctt gatagttaca ttatcttttc caaaagataa tcttgaaaat
     7381 cataaaataa atttatttgc acgtcaactt tgttcagata taaggtatgt taggcgaatt
     7441 aatatgttta gcaactacaa tacatatata tattatataa atcaaggtaa acacgaaggt
     7501 tatgtattga gaaaagatgg taaaaacatt aaatcaataa tgctacctaa aaatgcaaaa
     7561 atagctagga gtattgaaat aataaagttt aaatctgatg ggtcgcctca aaaggcgtct
     7621 acaatagaga tatataataa tagattaaga aaaactataa caataacacc tgtaagtgga
     7681 agagtacttc tgaaagaggg aaaatatgaa acataaatat ggttacttgc ttttggaaag
     7741 tgtcgtatca ttgtcttcaa tggtgataat tatactagtt ttatattcta tatttttgtc
     7801 tacaataaac ttaaagttaa aagtagaaga taagatagaa ctacaacaac aaagtcttga
     7861 aatcataaaa tctatggaag gaattattag caattctatg ggaataatga atgtatctaa
     7921 ttatgaagaa acatttaaaa agacaacatc tataaaatgt agatatttag atgaaaataa
     7981 taatgaagaa ggcataagta ataaagaaat tatattgaat gaaagacgaa ataagttatt
     8041 tgtaaattct ttaaatggcg aaagtagtca agcaggtggt tatgaaattg gagattatgt
     8101 agatgagatg tatgtatcaa ttactaataa tggtcaatat gttaatatta aattgaaatt
     8161 atcaaaacgt agtcaaaaat atgagactga tttcaaaata aaagtatgga attttagtga
     8221 aagtatataa aaatacataa atttaagaga aagaagtgac atcaataagt catataaaat
     8281 ataattattg agaactatat aactaaataa tatcaatatg taaagtgtat agattaagaa
     8341 tatagaggat aataataaaa atatagaata agtaatttgc caaatacttg tttatattgt
     8401 ataacttatg atattatgat attagtaaaa atttgtaatc ctgacttgac agaattcaag
     8461 taagagatat aattttaaag gcatagtaat gtaaaattta ctaaaaaata agaaatttat
     8521 tttaatatgg aggaacaaaa aatggtatca gcaggtgatt ttagaaaagg tgttacattt
     8581 gaaaaagatg gacaaccatg tttagtagtt gattttcaac acgttaagcc aggtaaagga
     8641 gctgctttcg ttagaactaa atacaaaaac ttaaaaacag gagctataag agaagaaagt
     8701 ttcaatccaa gtgaaaaatt ccctaaagct gttatagata caagacaaat gcaatatcta
     8761 tacaatgatg gtgagttata ttattttatg gatcaagaaa attttgaaca aataccatta
     8821 aactatgagc aagttgaaga tgcaattaag ttcttaaaag aaaatgaagt tgctacaata
     8881 agattttacc aaggacaacc attccaagtg gaagcgccaa actttgcaga gctagaagtt
     8941 acagatacag agccaggtat aaaaggtgat acagcaagta atgtaactaa agcagctaca
     9001 gttgaaactg gagcagttgt tcaagttcca ttatttataa atactggaga taaagtaaag
     9061 atagatacta gaacaggtga atatttatca agagtataaa aatttatgta tataaaagct
     9121 tatatgtctt aataatataa gatatatgag cttttagttt atatataaaa aagaaagcga
     9181 ggggtgttac atgaaacatt tatatgaaga agttgcatac ttaaaaggcc tagcagaagg
     9241 actagaaata agtgctgaaa gtaaagaagg gaaaatgata cataaaatag ttgatgcatt
     9301 agaagtattt gcagatgcta ttgtaacatt ggatgaagaa caagaagaac ttcaagattt
     9361 tgttgaatct atagatgaag atttagcaga tttagaagaa gatattgctg acatggaaga
     9421 agatctttat gaagaagatg acgatgagga tgatgaggac ttcagctata tagaaatgga
     9481 atgcccaaat tgtggagagt tagtagaaat agacgaagat ttgttatatg atgatgaagt
     9541 agatgttgtt tgtcctgatt gtaaggctgt gatattatca tcagaagatg attgtgatga
     9601 tgattgtaca tgtggtggtt gttcaagttg tgatgataga gagtaaaaat tcataagaat
     9661 taaaactgcc tatgatgcct atgagatatt ttgcttcata ggtagtttta tttttatgag
     9721 gaagggtatt atgtattttg gttttgcaat acttggacaa aaatagtaca ctagaattta
     9781 tagaatagga ttttggaggg atgtaaatat gaaaataagt aaaaaattac tagataatat
     9841 acaaagattt gaaaatgtta taaattataa atttaaaaat aaagaatata tactagaagc
     9901 tcttactcat agttcatatt ctaatgaaaa taaaaaatat aattttaacg aaagattgga
     9961 atttttaggg gattcggttt taggcatagt gataagtgat tatttattta atgaagaagc
    10021 taatctgccc gaaggggaac taactaaact tagagcaaat atagtttgtg aagattcatt
    10081 aagtgaagta gctaatgata taaatttggg tattcacatg ttattaggta gaggagaaga
    10141 agcaacagga ggaagacata ggacttcaat attagcagac gcatttgaag ctgtaatagc
    10201 tgctatatat cttgatggag gttttgaaag tgcaagacag tttatacttc atcatatgga
    10261 aaatataatt tatgattcaa gaaaaggaaa tatatttaga gactacaaaa cgcatcttca
    10321 agaggtgtta caagggaatg gagaaaataa tatctggtac aggcttatag aagaaaaagg
    10381 accagaccat aataaaagat ttgtaatgga agttggaatt aatgacgatg tacttggtat
    10441 aggagaaggc aagagtaaaa aagaagctga acagttagct gctaaaatag ctttaaagaa
    10501 aaaattatgg gaaaaataaa tataatatgt aaaaatataa aaagaaatat ttatattaga
    10561 aggatgttgt agatgaaaaa gagaattata cctatatttg ttcctcacaa aggatgtcct
    10621 catgattgca tattttgtaa tcaaaaaaaa ataacaggtg tgagtactga tgttactagt
    10681 gaagatgcaa gaaatataat tgaggaatgt ttagagacta tagataaaga tgcagatgta
    10741 gaaatagcat tttttggtgg tagctttact gctatagata ttgatataca aaaaagttta
    10801 ttatcagttg ctaaagaata tgtagaaaaa ggtcttgtaa aagatattag aatgtctaca
    10861 agaccagatt gtattgatga agatatatta agtatgttaa aagaatataa gacaagtata
    10921 attgaactag gagtacaatc cttagatgaa aaagtattat tagatagtat aagaggtcat
    10981 caatctgaaa ttgtatacaa gagttcaaag atgataaaaa atagtggaat caaacttgga
    11041 ttacagatga tgattggtct tccagctgac acagaagaaa agtgtatatt tacagcaaaa
    11101 aaatttgtag agctaaaacc agattgtgtt agagtctatc caactttggt agtaaaagat
    11161 acaggtcttg aaaagttaat ggaacaaaat aaatataatc catttactct tgaagagagt
    11221 gtaaatatag taaaaaaagt tcttgtactg ttttatgtta ataatatcaa tgtaataaga
    11281 gttgggcttc aagctactga tgatatacaa attggaaaag ctgttttagc aggaccatat
    11341 catcctgcat ttagagagtt ggtagaagca gatatgataa aagattattt agaatttgtt
    11401 attttgcaaa ataaaaatat aaagcaaatg ttggtcaaat ctaataaaaa gaatatttct
    11461 aaaatcatag gaaataaaaa aacaaatgtt aaatatatgg aagaaaaatt tggtgtgctt
    11521 ttaaaaacac aagaatcaga tttagatata aatcaattag aaattgtgtt agatgggaaa
    11581 agtttaatta gtgctaatat gagagacatt catagaaaat tgtatgacat ttatgacctt
    11641 tagttttaat aatgaaggga gagaaaagat ttgtatttaa aaagactaga gttaaaggga
    11701 tttaaatcct ttccagtaaa aacagatatt atttttaaag aaggaataac agccatagta
    11761 ggaccaaatg ggagtggaaa aagtaatata tctgatgctg taagatgggt attaggagaa
    11821 cagagtatta agagtcttag aggtgataaa ctcgaagatg taatatttgc aggtacagat
    11881 acaaaaaaac ctatgaatta ttgtgaagtg gctcttacta ttgataattc tgaaaatcaa
    11941 ttggaattag attttacaga agtaactata agaagaaggg cttatagaaa tggtgaaagt
    12001 gaattttttc taaataataa aagttgtaga ttaaaagata taaaagaagt ctttttagat
    12061 actggtattg gaaaagatgg ttattcaatc atagaacaag gaaaagtaga tgaaatatta
    12121 agtaataatc ctttaagcag aagaaaagtt tttgatgaag cttgtggcat atcaaaatat
    12181 agatataaaa aacaggaagc agaaaggaat ttaagtaata caaaggaaaa tttagaaaga
    12241 atagatgatg tatatataga aattgaaaat caattaaagc ctctttttaa tcaacagaca
    12301 aaggcaaaaa agtacttgga aataagtgaa aaattaaaga cacttgaagt aaatagcttt
    12361 ataagagaaa ttgaaggaat agaaaaagaa ttaagtgagg ttaatgaaca tcgtaaggtt
    12421 attgaaaagg agctcaatga aaaagaagaa caaaaaaatg ttgtagagaa aaagcaagaa
    12481 gatattaata aagaggtaga ggtattacaa gatgtaattg aaaaatctgt agattatata
    12541 aattcaatta aaggagttat atcaaaaaaa gaatcccaaa taaatttaat taaggaaaga
    12601 ataagaaatt ttacgaatga aattagtaga aaaaatttag aaataaatga tataaaagag
    12661 aagctaaatg aaaataagca atacataaaa gaattagaaa gtaataaatt atcaggtagt
    12721 gaagaactat ctaccttgca ggaaaatata aaagtattgg aaggaagtaa agataaacaa
    12781 aaaattaagt tagaatccct aaataatgag atagaattat tgaaagaaag tataattgat
    12841 atattaaata agaaacaaga gtttagtaat aagctgtcta ctttaaacgc aaataaagaa
    12901 aatatgaata tcagagatga aaatataaat tcagagataa cagagttaaa caaaaatatt
    12961 gaaataaaat catctgaatt agatactata aataaagaat ttaatatgca aaatgaaaat
    13021 ttaaagaatg taaataatag acataaagaa ttatcaatta atttgcagga ttcaattagt
    13081 gaacacaata aactagaaga tgaaatacag aaaagtaaat ataatttaaa tggatataat
    13141 tcaaagttaa atgtctatat agatatggaa aatcattatg aaggatttaa tagaggagta
    13201 aaagaagtac taaaaaataa aaatttaaaa ggagttcatg gagcattagg acagattata
    13261 aatgtacctg aaaagtatga gaaatctata gaagcagctc ttggtgctta tatgcaaaat
    13321 ataataactg ataatgagtt tagtgctaag tctgcaataa actatctaaa acagaataat
    13381 ttaggtagag taacatttct tccattaaat ataattaaat caaataaaat aagtctagga
    13441 aatttaaaag caaatactaa gttcatagga atagcaagtg atttaattac atttgatgaa
    13501 aagtatagaa atataattga aaatatactt ggaagaacaa tacttataaa taatatagat
    13561 gagggcataa aatttgcaaa agaaactggt cataggttta aaatagtaac tttagatggt
    13621 gaaatcttaa atccaggagg ttctttgact gggggaagtc taaaaactaa tgggaatata
    13681 ctatcgagaa aaagatacat taatgaatat acagaaaaga taagtaatat caagaatgaa
    13741 atttcacatc tagaattaaa aagagaatct ttagacaaag atgtgaaaaa tataaaaaat
    13801 gaaattgatt cacatgaaag taaaataaaa gatttagaaa aaagtataat aataaaaagt
    13861 acaagtataa agaatgttga atctgaaatt gaatcgttaa aaggtagtat tactaaatta
    13921 gaaaatgaga agaatgattt aaattctaac ctaaattata cacttgaaaa aagtgatgat
    13981 gttagaaaag atatggaaga gttagatgat ttatacaata aaaataaaga aaaaattgat
    14041 gcattgaatg aggaaataaa aagatacaat gatttatatg ataaagaaaa atctgaattt
    14101 gatgaattga acttaagttt agtgaagaaa acagaagttt acaatagtat agttagagat
    14161 ataaaaagaa tatctggtga aaattgtgag ttagaagaaa aaaataaaca attagaagag
    14221 tcattaaatt atgaagaaca tgaaataatt aaattacaag attctatact cactgaagaa
    14281 aaagaaaaag aaaatttaac aaaacaacta ggggatagta atagaaatct tgaaactaga
    14341 aaaatagcaa aagatgattt aaaaaatagt tttgatgaaa ttaataaaga attaaaaact
    14401 atagatagac agcatataga acttaaagaa agtttattta aagttggtgg aagattagaa
    14461 agattaaaaa ctagtcaaga cacatatata aataagttgt ttgagcaata tgatatgacc
    14521 ttagtacaag cattggaaat taaagatgaa gatttggata tagatagaaa attcttagaa
    14581 agtctaaaga gagaaataag aagtcttgga aatataaata tagactctat aaaggaatat
    14641 gaggaaataa aagaaagata cgatttttat agcgaacaaa agcaagattt agaagagtct
    14701 atggaagaga tagaaaaact tatacatact ttagaagaaa atatgaaatc tgaatttgaa
    14761 attaagtttg aagaaataag taaaaatttt aaatatgtat ataagagatt atttggtgga
    14821 ggttgtggag aattaactat tttagataaa gaaaatttat tagaaagtga tatactaata
    14881 acagctcaac ccccaggtaa aaaaatgaaa aatttaaacc tattatctgg tggagagaaa
    14941 gctttaacgg caataagtat attatttgct atattgatta caaagccaac accattttgt
    15001 atattagatg agattgaagc gccacttgat gacgcaaata tatttagatt tggagaattt
    15061 ttgaaggatt tatctaaaga gactcaattt atatcagtaa ctcataggag aggaactatg
    15121 gaagctgctg attatatcta cggagttaca atgcaagaaa aagccatttc aaaagttatt
    15181 agtttaaaat taaaagaagc tcaggaaata acagatatta tataacttag attgacatag
    15241 gaggaataat agtgttaaaa aaattatttg gttttggaaa agacaaggaa aaggaaatag
    15301 aaaaaaaaga tgctgaagaa gaaatagaag ttgaagacag tgttgataat ttagaaaact
    15361 tggaagaaac tattttttca ggacttgaag aagaagtgat agataaagtg gaagatgttg
    15421 aagaaaaaaa tgaggaaagt gtcaataatg aaggcactga agaaatcgaa aactttgaaa
    15481 aaatagaagt tgagatgaat agtaatggaa aagaagtaga aaatatttca aataatgatt
    15541 ctcaagaaga gataatcgaa gaattagaaa attatgatga aattgagtct aaagagatag
    15601 aagataaaga agataagaaa gttaacttgt ttgaaagact aaaacaagga ttgacaaaag
    15661 caaagcaagg aataactgat agaatagatg aagttttaaa atcatataca aagatagatg
    15721 aagaattact tgaagattta gaggaaattt taataacagc agatgttggt gtaaatacaa
    15781 ctatggatat aattgaaaga ctaagagata aaataaagca aaaaggtatt actgaaccaa
    15841 taaaggtaag agaagaactt aaatctatag ttgaagatat tcttacaaat gaaaactcta
    15901 cactagatat agaaccagca ccatgtataa tattaatggt gggtgtaaat ggggtcggta
    15961 agactactac aataggtaaa ctagcaaata gatacaaaaa agatggtaaa aaagttttat
    16021 tagcagcagc agatacattt agagcagcag cgacagagca attagaaatt tgggcaaata
    16081 gaaccaatgt agacataata aaacatcaag agggagcaga ccctggagct gtagtatttg
    16141 atgctataaa agctgcaaaa gctagaaaaa cagatgtatt aatatgtgat acagcaggaa
    16201 gattacacaa taaggctaat cttatgaatg aacttggaaa agtatttaaa atagtagata
    16261 gagaatttcc agaagcaaaa cgtgaagtac ttcttgtggt ggatgctaca acaggtcaaa
    16321 atgcagttgt tcaagcaaag acatttaaag aagttgcaga tataacaggt atagtactta
    16381 caaaacttga tggaactgca aaaggtggag tagtccttgc ggtaaaatca gaagtagatg
    16441 tacctgtaaa acttattgga gttggagaaa gtgtagaaga cctacaagat ttcaatgcaa
    16501 aatcattttc agatgcatta tttggtaact aatgttcttt taaaaataat ggttgacaag
    16561 agtgttgtta tgatataaaa tacaatagtg taaagtaaat aacctttaca gtatactgct
    16621 aggaagtgta agatatgaat atagaaaaaa tggtcgaaat tggattgtta tttgaacagt
    16681 ataaagaatt attaactgac aagcagaaag aaatagttgc tctatactat gaagaagatt
    16741 attctttagg tgaaattagt gaaaatctga atgtatcaag acaaggtgtt tatgatacct
    16801 taaagcgttc agaaaaaata ctaagagatt atgaagaaaa gttacactta gtatcaaaga
    16861 ttcaagagca ggaaaaaaat attaagatta taaaagataa aattattgac attaaagaag
    16921 atttattgca caatagagat tgtgctaatt taatccctaa gctagaaaat atagaagatg
    16981 tatgtaggga gatgataaaa tgatatttga aggattgtct gataaactac aaggggcatt
    17041 tagtaagtta aaatctaaag gaaaacttac agaagtagat gtaaaaaatg ctatgagaga
    17101 agttaaactt gctcttcttg aggcagatgt taactttaaa gtagttaaag atttcataaa
    17161 aaaagttcaa gagagatgtg ttgggcaaga agttatgcaa agcttaactc cagctcagca
    17221 tgtaatcaaa attgtaaatg aagagcttac aagtttaatg ggagatgtac aaagtaaagt
    17281 aatgatttcc tcaaagcctc ctacaatttt aatgatggtt ggattgcaag gtgcaggtaa
    17341 gactactaca gcaggaaaac ttggaggata ttttaaaaag caaggtaaaa aacctctatt
    17401 agtagcttgt gatatttata gaccagcagc tataaagcaa cttcaagttg ttggagaaaa
    17461 attggatata cctgtattca atatgggaga taaagaaaat ccagtaaata ttgcgaaagc
    17521 aggtttaagc catgcgatta aaaatgctaa tgatttagtt attatagata cagctggtag
    17581 acttcatata gatgaagtac ttatggatga gttaaagtct attaaatcag aagttaagcc
    17641 acatgagata cttttggttg tagattctat gacaggtcaa gatgctgtaa atgttgcaga
    17701 aagcttcaat gaggcacttg gtgttgatgg agtagtatta actaaattgg atggagatac
    17761 tagaggtggt gctgcacttt caataagagc agttacacaa aagcctataa aatttatggg
    17821 gatgggagaa aagctagatg atatagaacc tttccaccca gatagaatgg catcaagaat
    17881 attaggtatg ggagatgtct taagtttaat agaaaaagca caagaaaata tagacttaga
    17941 gaaagctaaa gaattagagc aaaaaattaa aaaacaagag cttgattttg aagatttctt
    18001 acaacaaatg gaacaaatac aaaatatggg acctcttgac aagatacttg gtatgatacc
    18061 tggtatggga aatgtaaaag accaacttgg agatgtagat ttgaatggca aagaaatgaa
    18121 gagaacaaaa gccataattc agtcaatgac tacatatgag agaagaaacc caggagtatt
    18181 aaatgcttca agaaagaaaa gaatagctaa aggcagtgga actagtgtac aagatgtaaa
    18241 tagactcata aaacaactta atgaaatgaa aaaaatgatg aagatgtttg ctggttcaca
    18301 gaagagtatg aagaaaaggg gcggcttagg cggtttacct ttttttaaat aaaatataaa
    18361 aaagtaaaaa ttattcggag gtgactgtaa tggcagttaa aataagatta aaaagaatgg
    18421 gagctaacaa aaaacctttc tacagaatag tagtagctga ttcaagagct ccaagagatg
    18481 gaaaatttat agaagaaata ggatactata atccaatttc tgaacctaaa caagtaagaa
    18541 taaatgatga aaaagctata aaatggttag ctactggtgc tcagccaaca gaagtagtta
    18601 aaaaattact tgtaaaaaac ggagtaatag aaaaattcga agcttctaaa caagcaaaat
    18661 aattttttct tggcaattag tttaagtaag ggctcattgc taaggttact ttaggagatg
    18721 aaatatatga aagagctagt tgtagatata gctaaggctc tagttgataa tcctgactca
    18781 gttgttgttg aggaatttga agataatgat ggtatcgtct taaagcttac agttgctcaa
    18841 gaggatatgg gtaaggttat tgggaagcag ggaagaatag ctaaagctat aagaactgtc
    18901 gtaagatctg ttgcaaatag agaaaatata aaagtttctc ttgagatagt ataaaatagg
    18961 aggattaggt ataatgccta gtccttttta tataaatgcg tagtttaaga ttttatgatg
    19021 atatgaccac ataagattaa attttttaag aaggtgataa catgaaagaa aaattaactc
    19081 attttaaggt aggtcaaata gtaaatacac aaggtttaaa aggtgaagta agagtttatc
    19141 cacttactga tgatatagag aggtttgatg aattaaaaga tttctatctg ggaaaagatt
    19201 taaataataa attggctgtt gaaaaagtaa gatacaaagg ttctatggtt ataatgaaga
    19261 taaaaggcat agacagcata gagaaagcag aaaaactaaa aaataaattt atgtatgtat
    19321 ctagagaaga tagcagagag cttgaagaag gcgaattttt tatagcagat atgattggaa
    19381 tggaagtttt aactgtagat ggaaaatatg taggaatact agaagatgtt cttcaatact
    19441 cagcaaatga tgtctatgta gtaaaaggtg aggaagataa agaatttatg ataccagcta
    19501 taaaaaaatt tgtacctaca atagatattg atgaaagaaa aatgattata gaccctatta
    19561 agggaatgat agattaggtg gtaatatgag atttcatata atgacacttt ttccagaaat
    19621 atttaattcg tacatggatg aaagtataat gaagagagca gttgaaaaag gtataattga
    19681 ggttcatata tataatataa gggatttttc taataataaa cataaaaaag tagatgatta
    19741 tccatttgga ggaggagcgg ggatggtaat gactcctcaa cctatatatg acacatataa
    19801 gcatataata acaactcata atataaataa cccaagtgta atttatctta ctccaaaagg
    19861 taaggtatac aatcaatcta tggcaaagca gatgtcttta aaagaagata taatactact
    19921 atgtggtcat tatgaaggaa tagatgagag aatcattgat ttaatagtta ctgatgaaat
    19981 ttcaattgga gattatgtat taactggggg agagctccca gcacttataa tgatagattc
    20041 tatatcgaga ttaataccag gtgtattaaa ccaagaagaa tcatttgaag aagaatcatt
    20101 taaagacaac ttattggaat atcctcatta cactagacct agagattttg aaggattaaa
    20161 agtaccagaa gtgcttttat cagggaatca taagaagata gatgagtgga gaagagaaga
    20221 atctataagg ataaccaaag aaagacgatt tgatttatat aaaaagtcta atgaaaaata
    20281 aaaatataat gaaaaattaa tatatatgta tacaataaag tacctataaa tggaattaaa
    20341 aacataaaag tgcttgtaat aactattgaa gtatagtata atataaaatg ttgaaaatac
    20401 gggcgttcct ctgtttacac tggtcgggta gttaagaacg tctatgatat ctaggaggaa
    20461 gaaagatgaa tgaaatgcta agagcaattg agcaagaaca attaaaaaat gaagttccta
    20521 attttggacc tggggacaca gttaaagtac acgttagaat tatagaagga aaaagagaaa
    20581 gaatacaagt attcgaaggt gtagtattaa agagacaagg tggaggagca agagaaactt
    20641 tcacagttag aaaaatgtct ttcaatgttg gtgtagaaag aactttccca gttcactctc
    20701 caaaaataga aaaaatagaa gttactagaa aaggtaaagt aagaagagct aaactaaact
    20761 acttaagagg tagagtaggt aaagctgcta agataaaaga agctagaaat aaataattaa
    20821 aaactttact tataaatggg gggctgattt atcagtcctc tttctatttt aatgaaaatt
    20881 acagcaaata attatactag aaataaatag aaatatttgt tgaaaatata cattatttat
    20941 ctatgaattt atagctttta aaatgaaaaa taaaattggt aaatattgca ttatattttg
    21001 taacaatatt aaaaataaga gaacagtatt atgtaaagta tgacgttaaa aagatgacaa
    21061 tattatagtt tgttaagata gattttttaa gtggaattag gatataataa tataatatta
    21121 ataaggagtg atacatgtgt caaatgaata cgaagagtat cttatgaatg acaatctaca
    21181 tataaactgg tatccaggtc atatgaaaaa aactaaggag ttagtaaaaa ataacttaaa
    21241 attgattgat gtagtaattg aacttttgga tgctagaatc ccgtttagta gtaaaaatcc
    21301 agacatagac aggcttgtag gagataagcc aagagttgtc gtacttaata agagtgatat
    21361 ggcagatagg gataaattga atcaatggat tgaatactat aagaaaatta atatcaaagc
    21421 tataccagta gatacaataa aaggcgttgg tataaataag ataatcgaag aatgtaaaaa
    21481 tgtaactaga gaaaaaatga gttctttaaa agacaaaggg agaaaagaaa gagctatacg
    21541 aataatgata gttggagttc caaacgttgg taaatcgtct cttataaaca aattaacagg
    21601 tagaaaaagc actcaaactg gagataagcc aggagtaacg aaagggaaac aatgggttag
    21661 attaaaggga aatcttgaac ttttagatac acctggaata ctttggccta agtttgaaga
    21721 ccaagaagtt gcattaaatt tagcatttag tagagctata aaggatgaga ttttagatac
    21781 tgaaactttg gctttaagac ttatagaaaa acttatgaaa atagaacctg agaagttgaa
    21841 agccagatat aagttagatt gtctaggaga aactccaatt gaaactatgg atatgattgg
    21901 ccataaaaga gggtttataa caggtaaaaa agaactagat tatacacgca tagccacaac
    21961 tgtattaaat gaatttagag atggaaaaat aggaaatata acattggaag ttccagaaaa
    22021 cgttaaaaga tagattttaa aacaaaatca aaaatttaat gttaaaaatt taaaaaagcc
    22081 ttttagcaaa ggctttttta tttttttgaa atatagacaa atatctccat ataaaagtat
    22141 aaattaggtt agaaaacctt taaaaatatt aatttaagac aaatatacct aatattttga
    22201 aaattaaaat gagtgtgatt atttaaaatt ttagcatatt gacttgcttg tttcgtataa
    22261 tatataataa gcacaaataa atacaatata ttaaaaattt aatttattta attttattaa
    22321 tttaataata agttcttatt gtttaattca acttacttat gcaagcgtaa acaatcgaga
    22381 cattttatta atttaataag gttaaaaagg gggattttat aatgggtaag accaaggatg
    22441 ttatagtatt tggatttgcg ttatttgcaa tgttctttgg agcaggtaac ttaatatttc
    22501 caccttactt gggaataatt acaggtccag aatggttaat tgccttctta ggatttacgt
    22561 ttgctgatgc tggattggca cttctagcag taatggctac agctaaattc gatggaaacg
    22621 tagttgaaat gtttaaaaga tgtggtatga agttaggaat tcttataggt tgtgcagata
    22681 ttttatgtat aggcccattc ttagctatac caagaacagg tgcaacaact tatgaaatgg
    22741 gtataatgcc tttgtttgga acttcaattc cagtgttatt attctgtata cttttttttg
    22801 cgatttcata tgtactaaca ataagacctt ctaaggtcgt agatatagtt ggacagtttt
    22861 taactccagc tcttctaata gctttagcat ttataattat aaaaggtatt atatcacctc
    22921 ttggagatat agtagataaa cctatgatac ctaatgtatt tgcagaaggt ataggtcaag
    22981 gatatcagac aatggatgct tttgcagcaa tagcacttgc atcagtgcta atagtatctt
    23041 taaatgataa aggatattct actattagtg acaagttgaa aatgattggt aaggcgggtg
    23101 ttcttgcttg tggaggatta gctctagttt atggaggatt atgtttcctt ggggcaactg
    23161 tatcaacaat gtatggaaca gatgcagtac aatcacaagt aatagtaaat ataactgaag
    23221 gattgcttgg aaatgttggg aaagcgattt tagcagtagt agtatcatta gcatgtttaa
    23281 ctacatctat aggtcttaca tctgccacag gtcaatattt ctctagactt acaaaaggta
    23341 aattgagtta tgaaaaaata gtattagctg tatctgtatt cagtgcagta gtagcatctt
    23401 ttggtgttgg aactataata aagatagcat ctcctatact aagtatagtt tatccaccat
    23461 caattgtttt aataatttta gctttcttca atgaaaaaat taagaatgat aatgtataca
    23521 aaggagcagt atatatgtca ttgcttgtaa gtatactaac agttatatct agttatggag
    23581 tagctgttcc agtagtaaat tctttacctc ttaattcatt aggatttaac tgggtggtgc
    23641 cagttataat agctggaata attggtaatt ttataccatc aaagagtcaa tctaatacac
    23701 ttggaacaaa ctaagttttg tttaattaaa atataatcaa ttgtaaataa atgagttaac
    23761 taaaagtaaa ag
//
LOCUS       DAGSWI010000056        23222 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.056, whole genome shotgun sequence.
ACCESSION   DAGSWI010000056 DAGSWI010000000
VERSION     DAGSWI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.056"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            273..1211
                     /gene="pdaA"
                     /locus_tag="MJF80_002032"
     CDS             273..1211
                     /gene="pdaA"
                     /locus_tag="MJF80_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBZ0280693.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
     gene            complement(1583..2956)
                     /locus_tag="MJF80_002033"
     CDS             complement(1583..2956)
                     /locus_tag="MJF80_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBZ0280694.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(3143..4465)
                     /locus_tag="MJF80_002034"
     CDS             complement(3143..4465)
                     /locus_tag="MJF80_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0280695.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            complement(4458..5162)
                     /locus_tag="MJF80_002035"
     CDS             complement(4458..5162)
                     /locus_tag="MJF80_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0280696.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            complement(5345..6409)
                     /locus_tag="MJF80_002036"
     CDS             complement(5345..6409)
                     /locus_tag="MJF80_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBZ0280697.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            complement(6412..7110)
                     /locus_tag="MJF80_002037"
     CDS             complement(6412..7110)
                     /locus_tag="MJF80_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBZ0280698.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYNYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            complement(7101..8198)
                     /locus_tag="MJF80_002038"
     CDS             complement(7101..8198)
                     /locus_tag="MJF80_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBZ0280699.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            complement(8521..9765)
                     /locus_tag="MJF80_002039"
     CDS             complement(8521..9765)
                     /locus_tag="MJF80_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBZ0280700.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            10093..10881
                     /locus_tag="MJF80_002040"
     CDS             10093..10881
                     /locus_tag="MJF80_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBZ0280701.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            10881..11354
                     /locus_tag="MJF80_002041"
     CDS             10881..11354
                     /locus_tag="MJF80_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBZ0280702.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(11446..11886)
                     /locus_tag="MJF80_002042"
     CDS             complement(11446..11886)
                     /locus_tag="MJF80_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBZ0280703.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11901..12194)
                     /locus_tag="MJF80_002043"
     CDS             complement(11901..12194)
                     /locus_tag="MJF80_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBZ0280704.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            complement(12237..12638)
                     /locus_tag="MJF80_002044"
     CDS             complement(12237..12638)
                     /locus_tag="MJF80_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280705.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            12871..14991
                     /locus_tag="MJF80_002045"
     CDS             12871..14991
                     /locus_tag="MJF80_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0280706.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKKSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            15216..16382
                     /locus_tag="MJF80_002046"
     CDS             15216..16382
                     /locus_tag="MJF80_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBZ0280707.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVVLPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            16407..17849
                     /gene="nhaC"
                     /locus_tag="MJF80_002047"
     CDS             16407..17849
                     /gene="nhaC"
                     /locus_tag="MJF80_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBZ0280708.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            17852..18121
                     /locus_tag="MJF80_002048"
     CDS             17852..18121
                     /locus_tag="MJF80_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280709.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMRGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(18198..19625)
                     /locus_tag="MJF80_002049"
     CDS             complement(18198..19625)
                     /locus_tag="MJF80_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBZ0280710.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            19997..20866
                     /locus_tag="MJF80_002050"
     CDS             19997..20866
                     /locus_tag="MJF80_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBZ0280711.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            complement(20978..21784)
                     /locus_tag="MJF80_002051"
     CDS             complement(20978..21784)
                     /locus_tag="MJF80_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBZ0280712.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(21798..23030)
                     /locus_tag="MJF80_002052"
     CDS             complement(21798..23030)
                     /locus_tag="MJF80_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBZ0280713.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
BASE COUNT         8113 a         3675 c         2886 g         8548 t
ORIGIN      
        1 attcaattaa cttctttgtg cttgttattt tgttacttct tgttgagcac atttattata
       61 ataacatctt ctcataatat ggtcaactat ttttttcatt ttttttaaat ttattgatta
      121 ttcacccttt gtcatactat tgtgacttta tttttaaaga tataactatt attaatagta
      181 atttccaatt ataataatac tatatgcaat tgtaattaat catataatta ttattatatg
      241 caattattat taatttagaa aggagcttca aaatgtttag aaaagtattg cattatttaa
      301 cactactttc tatttctatt ttttttattg ttggatgttc aaattcacaa aacaatcaaa
      361 acgaaaatca aaataaggaa acacagttac aagaagataa ggaaaaaata gatagtggta
      421 aagatacttc caatgtaata gtttcagacg gaacagataa accatcaaaa gccactacta
      481 ataatgataa taataaatta gatgtatctt ctttagataa cactactctt gattggtttt
      541 atataccaaa caataagcat aaaacaccag aagtaaatac ggacattgaa tttaaatttt
      601 ctgactatga tgccctatac aatggaccta caaaagatgg tcaaaaaaca ctatatctta
      661 catttgatga aggttatgaa aatggataca ctacaaaaat actcgacact ctaaaacaaa
      721 accaggttaa agcagttttt tttgttacag ctccatatat aaaagaaaat aaagatttag
      781 taaaaagaat ggtttctgag ggacatatag ttggaaatca tagtaaaacc catccatcta
      841 tgccaacaaa aacaagcaat ctaaaaaact ttaatgacga attatatgat gttgaaaagt
      901 tatataaaga tgtaactggg aaagatatgg ttaaattttt tagacctcct atgggaaaat
      961 actcagaaaa aagcttggca atgactaaaa atttaggata caagactgta ttttggagtt
     1021 ttgcatacag agactgggat actgataaac aaccatcaca tgaagaagca acgcaaaaaa
     1081 taatggataa tctacatgat ggttctatct tgttactaca tgcagtttcc aaaactagta
     1141 cagaaatttt aaatgatttt atatcaaatg caagaaaact aggatatgaa tttgaactct
     1201 tggagtatta atatttataa ttacatctaa agtctacaaa ctaatatagt taatataata
     1261 taggttataa aataataaat taaaattaga atttttgttt ggtgatagta taggtaaaaa
     1321 cttgtgatag cttatatatg ttggtgggac agggcaataa aacatcaaaa aatttggcaa
     1381 gatattaaat atagataaca gtattttaga tgttatattt agatgttatg tataatataa
     1441 tgaaaaatat tactttatta aagaatacta ataaaaatat caataaaaat agatgtatgg
     1501 cttagaatta gtaaaacaat tataataaat tatagctaca catctatttt taatttatac
     1561 aattaaagat ttattcgatt tattaaataa taaacattgc tactatggtt gttacaacca
     1621 atccggttat aactgggaca aagtttcgct ttgccaattc tattggacta accccacaaa
     1681 ttgccgctgc tgaaataaga ccccaaggaa ctagacagcc tccacctacc catataccac
     1741 tgatttgtcc taatgcagct aatgcaccaa cacttgcatt tatagcagtt ccaaatacag
     1801 aggcaagtga accaactaat gaaatcccag aaaatccaga accatcaagc ccagttattg
     1861 ctcccactac agtttctata cctgatacgg caaatttatt aagtggtatt gtttgagaaa
     1921 gagctatgcc tatatctgat aataaacctt gtgaacctgg tgctaatact tttccaaata
     1981 cacctgtcaa tggtgcaact tcacccatat agaaaaatgc tgctattggt ataattgctc
     2041 caaatatttt tatcccaaac acaaaaccat caattatatt ctcacataca ttttcaagac
     2101 tatctttgcc aaaatttata acatttataa ttattattaa aaatactgct gttccaccca
     2161 aaagagctga tgcatctcct ccttttaggt caaatacata catagcaatt atatcagcaa
     2221 gaaatcccag agctactaaa atagttgcta ttttagattt tccattgaac tcttctactt
     2281 cttcactttc aaaacctttt agttcttctc taaacattcc tttgttaata tctcttttta
     2341 atgtataaaa tgccatagat atggttacga ttcccatcac ccaaaacaaa atcataccat
     2401 catttataac atatgatact gcaacacctg ctgcaccagc tgttatactt ggagctcctt
     2461 gaataacaaa gtctgtagac aaggcaagac catgaccaaa taaatttaat gctactgcaa
     2521 caccaattgc tggtagccca gctctcatag caacaggtaa aaatacagct ccaactaagg
     2581 caacagctgg agttggccag aaaaaccaag aaagaactaa cataacaaat ccagttacaa
     2641 aaaatgctgt attagaattt tttataactc tcgaaaatgg tctcaccata tattgtatag
     2701 cattattggc ttctagggct ttagataatg caaccataat agctataatc aagataattg
     2761 gacccaactc atttaaagat ataataaaag agttaaacac tgctgttata gcaccaaaaa
     2821 tgtttttttc aaagaacaat cccataaaaa aaacacctaa tatacatggt actattgtat
     2881 cctttttcat aatcatagta attaatataa ttaccataaa tagaatgtaa atataatgtg
     2941 ctcctgtcaa aaccatatta ttatgtcccc tttttttaag ataataatat tatatggtct
     3001 tgtaagtaaa cttgttactt gcctagaggt acacaatatt gaacatccca taactaaagt
     3061 cacatgcttc ctgcttcata gaattttaca taagcaatta cacaatatat aatagcaact
     3121 acacaatata taatttaaat ttttatgatt cttttgctgg aatagatatt tcaatagcaa
     3181 attctttatc atctgaattt acatttatgc ttccattatg atatgcaact attttctcac
     3241 aattctttat accaattcca aaactttcta cattcttaga aaaattactt actctatttt
     3301 tttgtataat tagtaattta gaatcctcta ctttaattaa aaatactata ggcttatctt
     3361 tcctagcata ttttattata ttagaacata aattatcaaa tatccttcta aattgtgtaa
     3421 catctacata aatatgatat tggttataaa gttcattttc tattgttata tgaaatcctt
     3481 tctcctccaa tgtatatgaa ctatctaaaa gtaattgaaa aataaattca ttagcattta
     3541 ttttttcata cccttttgaa acttcctctc cagaattaac aaaatgcgta aaaaggtcat
     3601 ctgttaaatc ttttatttga tatgctttag accttacatt ttttatgtat tgttccaatt
     3661 gctcaggact attgtattta tggtctttaa tcaaatctaa ataacaaatt actgacgtta
     3721 atggagttct aacatcatgt gatatcgaag ttactatatc tttattttta ttcttcatct
     3781 ccctttcttt tataagttga ttctccaatg tatgactcat ctcattaata ctagatgcaa
     3841 gtgatgataa ttcatcatta ccttttactg ggattctata ctttaaactg ccactctcta
     3901 ctatctttat acccatttct attttcctta ggtactttat ctttctacta aacaacagta
     3961 aaaatgttat aataaatgta agtgccataa atgtaataga aattgcttct ataaaatttt
     4021 gaaatctaaa tctaaataaa gctactacaa ctatattagc tttaatatca gaaaattgta
     4081 tctcatagtt tctagccatg ggactgctat aaaactcatt ttcctcactc ttatcaggta
     4141 cttcattagg atagtataat gtatcataaa aaaccttatt tcctttatat atcttcatag
     4201 aaacaaacca attatcacta ttccattttg ctatatcatc tccatcatct gctcttatac
     4261 cattgttatt cacataagtt tgaaaattat ccataagttg atttacttct cttttaaaat
     4321 atactttccc ttctgataca tcttttacta tagattcaac agcagagtaa aatggtatat
     4381 ttaaaatgaa agccactgca gctgagatta ctacatatat caatagtata actcccaact
     4441 tagatggctt aattttgtta atcaatataa tacccctttc cccatgcggt ttttatatat
     4501 tgtggatttt tttggtcttt ttctaacttt tttcttaaat ttctaatatg aaccattatt
     4561 gtattatttg ctgtataaaa atactgctca tcccaaatag tttcatataa attttcagac
     4621 gaaaaaactt tttttctatt tttcaacata agaattaata tactatattc tatagttgtc
     4681 aaaaacacag gttctccatt tagtgtaaca tcttgagtat ctaaattgac acataactct
     4741 tttgttttta atatatttgg ctctctttcc ttttcctttc cctggtatat ataataacgt
     4801 ctaagtagag acttaaccct tgaaattagt tcagaatatg aaaaaggctt tgataaatag
     4861 tcatctcctc ctgctgaaaa tccaataact ttatcagagt cttgagtttt tgcagttaaa
     4921 aataatattg gtgctgtcgt tttttctcta atttcattac atgcaacaaa tcctgttttt
     4981 acaggcatta caacatccaa aataatcaaa tcaatcgtac tatctactaa gtttacagct
     5041 tcttctccat tcgttgccat aataacttca taaccttcac cagttaaaag tatttctacg
     5101 atttccctaa tctcaggatt atcatcagct actaaaattt tttttggaat ctctttattc
     5161 atcaccaagc tccttttttt gctatatttc ttaaatattt caaactatat aaaatattat
     5221 actttattag caaaagctta gcaatgtcac acgttttaat agtttctact cctgtaaaca
     5281 tgtttaaagc ttaattaaaa tatacacaca aattgacata ttaataaaaa taatctatat
     5341 aatattatct tacataagca acttttttca ctgcttttac tttttcagaa gattttacat
     5401 gtactactat acatatcaat ccacttatag ctacaaatgc ataaaaactt atccccctac
     5461 taagtaacat tgccggaact aataagtgac cagggaaaaa tatcttaaat aatgtcataa
     5521 aactattttc agaagctcca acagcccctg gcaatggtaa tgaagaaaca gcaatattaa
     5581 gcaaggattg tatcgctaat atctcaaata cagtatatgt tgatagatga aaagctttgt
     5641 atacaaagaa aggaactaaa aacatagctg ttatttgtac aatagttatg actaaaattt
     5701 ggactacaag ttttggattc tgctttatat attgagcacc tttaacatac tcttcaattt
     5761 gagatattat aacttttctt gaactttcaa catcttttat tattcttaac ttacctaata
     5821 aatttgttat tgatattata aaattgttta caagtttttt tgaaaatata attgcaatta
     5881 ctccaatgac aagagcaaca tttgttatta caccatatat tagtaaaatg ttcataccac
     5941 ttacattatt taatataaac tcacgttcca taatgaacat tattccacta tatgccagta
     6001 taactacttg gtgaataaca acaatgacta atagtataag tgaagaatat gatatattta
     6061 tatcgtcttt tttcatataa taaacttgtg ctggttgacc acctgatgct gaaggtgtaa
     6121 tagaactaaa ataaaatcca ataaaagcat atcccataca ttttaaaaga gtagtctttt
     6181 ggtttaaagc tttcatacct aagtatatat ttataccttc acaggcaaca tatataaaca
     6241 tcattgcaaa tccaattagt ataaacatag ggcttgcacc ttttaatgat tttatcaatg
     6301 attcaactga ctgccctcta aaaacaaaat atcctgttat gcccattagt aaaactaaaa
     6361 aagctacact acctaggcat ttatttttct tttcagtttt acttatcata actacatcct
     6421 ttctggtgtt acgaacttta aaccactggc tttaagtttt ggaatagcta tctttaaagc
     6481 ttcaatagta cgctcaggtg cacctactgc accacctgaa ttttcacctg catcatgtaa
     6541 acaaattata gaattttctt ttgttctact cattaacttg tttataataa tgtctactgt
     6601 actatctttc tcccaatctt ctgccattac atcccacaaa gtcattttta gattatattt
     6661 tttaacaaat gaatttgaaa atatattagt atgaccccat ggtggacgat agtagtttac
     6721 atctacacct aaatttttca ttatatttgt actttcaata aaatcttttt taacataatt
     6781 ataactataa agccatgcat ttctgtgttc caatgaatgt aaaccaacta tatgattttc
     6841 tctcaacatt ctttttataa tttcaggctc tttttcagca ttctttgcta ctacaaaaaa
     6901 catagcttgt atatcatttt cttttaacac atctagtaat ttttctgtat atctcttatc
     6961 tggaccatca tcaaatgtaa gtgcaatttc attttctcca gccatatttt ttaatacctc
     7021 tttatttaat agtttattat agtatgtagg tataattgaa tggactaaaa aaatcaatgc
     7081 aacaacgata attaaaccta ctacatacat tctctacacg ctccttgttc atcaagtgaa
     7141 aataaaatat tctctaaggt attattatca aattgttttt ttaattcttt catgttactg
     7201 ctcatttctt taagagtagc atcatcataa attaaatctt ttatctcatc tacatagtat
     7261 tttttatttt taccaagtat tctcccaatt tcattattta atataaaact agcattatct
     7321 atctcttgtt gtaaaaatgg attaaatgcc aatattggaa gttctgaata aattgtttca
     7381 aatagtgtta ttccacctgg tttagaaata ataaggtcag aatctttcat atatttatat
     7441 acttcattgg tgtaacctac tacctctata ttttcatatc taccatatag tttatagtac
     7501 atttttttat tattaccagt tattacagta gtttttactc cttctaaact atttaattct
     7561 ttgtaaaatt gttctgattt aggcagtaat cctaatcctc cacccattat aagaatattt
     7621 tttttagttg aatgatttac atgttctatt cttttaaatt ctttttttac tggaatacca
     7681 ttaactttaa tcttagattc atctattcct ttaaaaacaa gttcttcctt agtagattta
     7741 ctagctacta aataacagtc tgtattttta ctaatccatt ctgagtgact acttatatca
     7801 gttatacatg ttattaaagg aagatttgag ttatattttt ccttatatct tgatactaat
     7861 tgagaacaaa atggaaaagt tgaaataact actgttggtt gtacttcatg aagtaatgta
     7921 tctaatttat ttaaaaaata atctgaaaaa gtaaatttta tatctttctt tccattttca
     7981 gcacatttat aaaataaatt ataaagacta cttccccgat ttacaagaat agaaaaagtc
     8041 ttgtacattt tatctgaata gttaggcata atgtactcaa atatatcttt aattattatt
     8101 tctgaattat caaattttgc ttttatatct tcacttaaag aatttgatgc tgagtaatga
     8161 cccataccaa attttcctgt aagaattaat accttcataa gattccctcc tcgttaaagc
     8221 ttttcttttc atatgattac tatacttata agtatagact ttaaatcttt agaaaaacct
     8281 agtataattc ttaagaaatc ttaaagtttt aattaaaaaa tacaaagaga ccatctaaaa
     8341 atagagataa ccatattttt tattatttaa ataatacaaa tatgttcgat tccatacaaa
     8401 aaagtgcctc ataaactaaa aagttcattt tgagacacct tttttatatc taaatatatt
     8461 caaatacata cacaatcatt gtaattattt aaatgtattt tatttagttg ttctttataa
     8521 ttaatccata atattatatt cttcatataa aggatatttt tcagttaaac caacaaccaa
     8581 tgttctagcc ttagattctt catcataagt taaacatagg tctatagcct cagcgattat
     8641 tgccatatct tcttctttca tacctctagt agttacagct ggagtaccaa gtcttattcc
     8701 acttgtaaca agagcaccat ttgggtcaaa tggaattgta tttttattag ctgttatata
     8761 agcatcatcc aatctctttt ctgctgcttt accagttata tttttattag tcaaatctaa
     8821 tagtattaaa tgattatctg tacctccaga aatcaattta aaatctcttt taataagttc
     8881 ttcagctaag gcttttgcat ttttagcaac ttgtacctga tattctttaa actcatcact
     8941 taaagcttct ttaaagcaaa ctgccttaga agcaattata tgttcaagag gtccaccttg
     9001 aataccaggg aatattgctt tgtctatgtc tttagcatat ttttctttac aaagaataac
     9061 acccccacga ggtcctctta aagttttgtg agttgttgtt gtaacaaaat cagccacttc
     9121 acatggattt tggtgaagac cagcagccac aagacctgct atatgagcca tatctaccat
     9181 aagcaatgct ccaacctcat cagctatctc tctaaacttt ttaaaatcta tttctctagg
     9241 atatgcactt gctcctgcaa ctatcatttt aggttttact tcatgtgcta tctttctaac
     9301 ttcatcaaaa tctataagtc cactgtcttt atctatacca tattcataaa atttataata
     9361 ttgaccagaa atattaactg gagcaccatg agttaaatgt ccaccttgag ataaattcat
     9421 tcccataaca acatcacctg gttttaacat agcaaaatat acaccaatat ttgcatttgc
     9481 tccagagtga ggttgaacat tagcatgttc tgctccaaat atttttttga ttctatctat
     9541 tgcaagagtt tcaacttcat ctatgtattc acatccacca taatatcttt tgcctgagta
     9601 accttctgca tacttatttg taagatgact tcccatagtt tccattactg gaactgatac
     9661 tatgttttca gatgctatta attcaatatt tctttgttgt ctttttaact ctcttttcat
     9721 actttcataa atctcagggt ccgctttttt caagttctca aacatattac tcctcctagt
     9781 tattatttaa attttatttt tacagacatt tcataatttt cgtttttatc aatatgataa
     9841 tttattatta tagtccttta ccaattaata gtcaagtgtt ttgccatttt tccatattta
     9901 acatagttac tttctttatt tttaagttaa aatataataa ataatattgt ctaattcttt
     9961 taattggata taatttaaat gtgactattt tgaaacaaaa aaaagataat atatttatat
    10021 agatattaag gaatattact taaaaatttg atataatttc aatattacgt tttttaattt
    10081 ggaggtgtat ttatgaatca aaatgaggaa cttgaatata agaaaaatat tctaaaacta
    10141 gctctatttg taggggaact gatgataaaa aatggagctg aaacatctag ggttgaagac
    10201 agtgtcctta gaatatgcag gtcaagaggg ttttatcatg taaatatttt tacaactcct
    10261 acagtaataa ttatttcaga tgagaaattt gatggctttt cgtttatgaa aacgattcaa
    10321 tcaagaggta taaacttaaa taagatttct ttattaaaca gtttttctcg agaatttgtt
    10381 agtgacaaag agtatgatat tgataatgcc attgcacgtc tttatgaaat ccaagatgta
    10441 aaaccttatc ctttatggtt attttactct tgtacaggaa ttgcatctgc ttgttttgct
    10501 tgtttgcttg gagggaatta tgtattaaat tttatactta cgtttataat agcaatcttt
    10561 gctgcaatta cttatgataa aacgatgaag ataagtgcaa tatctgcatt ttcctgttta
    10621 gtatcttctt ttttaattgc actttcagga gttctactag ttgaaatagg cattttagat
    10681 gaccctaaaa tgcttatagt cggcgctata atgccactac tcccaggagt tgcttttata
    10741 aaagctatta gagatttaat atcagggaat ttaatatcag gaatttctcg tgcttttgat
    10801 gctgctatga ttattattgc aattgcttct ggagttggtt ttgtccttga tacatggtat
    10861 agaataggag gtcctttcta atgactgata tgtctatgat tttgcatttt ttattttcag
    10921 gtgttgcaac tgctggattt gctgttttct ttaatgctcc actatatcta cttcttccag
    10981 ctggagtaac tggcggaatt ggttggattg tatatgttta tttatttaat tttactacaa
    11041 atgcagtttt tgcaggtttt atcgctgctg ctttggtctc agcgtgcagt gagactttag
    11101 caagaaaact aaagcaacca gctattgtgt tcgtaattcc aggtattctt cctctaatac
    11161 caggaattgg gctatataat acaatgttgt ctttgattca gaaaaattat tctttggcta
    11221 tgtcaaaagg tacagatgct ctatttctaa gtgctgcaat agcacttggt gtattagttg
    11281 taacttcttt tgtaagaaca ttgaatcttt taaaaatcag aaaaaacttt attccttaca
    11341 gaaaatcaaa ataaaaatat aaaaatagca atgtacttaa actctacaaa tacgtattga
    11401 gtatttattt aaaaagtaca ttgctatttt ttaattataa ttatcttaaa ggtcaattaa
    11461 tattcttgtt attactggta taaattctct actgtctcta aattttttgt cattcttctt
    11521 taagtataaa taacagatta ttgtaagaca aattccaact aatccactta tggcttcaac
    11581 tccagttgtc attccaattg cccccaatat ataataagtc cctattgttc ctacaagaag
    11641 tgctataaga ggtattatat aagccattac agctgctttt aatacattca tattatccat
    11701 actcacttct acatggtcac caacttttgc accaattgtg ttgtctactt ctactaatat
    11761 ttcttttgac tctgctgaat ttgtagtagc acattttcca caagcagcac aagctgaatg
    11821 tctttgcatt tttaactttg cagttctatc atcaacaatt tccataataa atccttgttg
    11881 attcataact aaccgcctcc ttattttgta aatttattta tagtatccat aagctctttt
    11941 atcatctctt cagttttatc agcatcatca tgtttaatgc tttctgctac acatcctttt
    12001 atatgattct ctaagataat tgtgcctact ctatttatag cagaccttat agcagatatt
    12061 tgaaccaata tatcaacaca ataacgttct tgttccacca tcttttgtat acccttaact
    12121 tgaccttcta ttctttttaa tctttttatg agagcttccc tctcattgtt tggtgctaat
    12181 tttctttctt ccataaactc acctcatact ctaatgatgt cttatacaat tatgctctac
    12241 ttaccaactt gtttaaaata taatacagtt attattacta taaatgccaa aattgatatt
    12301 gcaatagacc tactttgatt cttagtttgt tccatactta actttccatt catcttaaat
    12361 cctgccaatg ggaataaaaa tataaccagt gcatcaattc caagtacaac ttgggctgca
    12421 atagtccatt tactcaaaaa gcagaatatt ccgcccacac ctaaaagaac ctctaatata
    12481 tttataaact tatatgtagg tttttttgct atatattctt caattttttc cttacattct
    12541 gcacgacatg gatatatatt catattgtca tcatatttta tagctaattt attatttggt
    12601 gactttatat ccctattaca gtaaagacat tttttcattt tatattctcc tctagtcttc
    12661 cttaaaaaat tttgcctaaa ttattatacg ctctaatact attataccaa atagatacaa
    12721 aaaacaaaaa taaaaggaat aattttagct acttctttta cttcaatgtg tagtctataa
    12781 taaatttata ataataatta aataatatta tttaatctaa tttaattatt atgtaatcta
    12841 aaataagtca aatttgtatg agaggttaaa atgaaacaaa tggaattagc tattgtaagt
    12901 ttaaaaaaag acgctggaga aatttatgaa aatcaaataa gacaattctt aggtgataat
    12961 ctaaagataa atttatactc atttgaagag ggaaatttaa agttttttaa agaaaaactt
    13021 atactcctat ctgcttattt aaaatatgat gaaatagtaa aattatctca ctatgatgct
    13081 caaatcattg ttcccaagtt gacatttgaa aagaacagta ttgatatgat atccaaatta
    13141 gaaaaagata aaattattta tgtatataac ttaagtaaag atatggcaat agaaacaatt
    13201 tctttaatac atagattagg aatagacaat ataaatatcc tgccctgcta tccagagatt
    13261 gagtttactc ccacagatgc tgtaatccta actcctggcg aaaaaatctt gcctaagttt
    13321 aaaaattgtg aagttataga tttaaaatac agaataatag atttaagttg tattgttgaa
    13381 attgctacaa aaaccaaatt aaaacatcta ataaaagatg acctaataaa aaaatacgta
    13441 gaaaaaataa ttcctacaag ttatagtaca ggtgagttat tgttagatgc aaataagttt
    13501 gaaagacaat ttgatttgtt gctatctata attgatgatg gaattatatg cacaaataac
    13561 gatggaataa ttcaattcta taatcatatg gctagaaaaa ttttgtctat aaatgcaaat
    13621 gaaatgattg actcatttgt tggtgattgc ataaaagaca tcaattttca aagcatattg
    13681 accaataaaa ctccattttt tgagaaattg attaaaataa atcatattga tataaattta
    13741 gagattaaac atatacaatt aaatgtattt gatggattta tactaaagat gacaaagttt
    13801 tctcaattag aaaaaaagca agctaaatta agagcacaac tagtaaactc tggtaatatc
    13861 agtaaataca catttgatga tatattaggt tctagtatac aaactattaa tacaaaaaaa
    13921 attgctaata aaatggctca atcaaattca tctatcctta taattggtga aagtggtact
    13981 ggtaaagaat tatttgctca atccattcat agtgcttcta ggagaaaaga tggtcctttt
    14041 gtcgcagtaa attgtagtac ttttcaagaa aatctactgc aaagtgaatt atttggctat
    14101 gatgaagggg cttttactgg tgctaaaaaa ggtgggaaaa ttggtttatt tgaattagcc
    14161 aacaatggaa ctatattttt agacgaaatt ggagaaatgg atttaaattc tcaatctaaa
    14221 ttacttagag tcatacaaga aaaacaagta cgacgtattg gttctaataa tgtcattgat
    14281 attgatgtaa gaataattgc agctactaat agaaatttaa aagaacttgt atctaaaaat
    14341 atgtttagaa gagatttata ttttaggttg aatgttcttc cccttaaaat acatccactt
    14401 agggaaagac ctgctgatat atttgaaatt tttggttcat taaaatatga tattccatgt
    14461 aattttattc taagtgagga agtaaaagaa atctttaaaa tgtatagatg ggaaggaaat
    14521 gtaagagaat taagaaactt aggagaatac ttctgttact tggaaaaaga tattatagaa
    14581 atatgtgatt tacctgaata tatattggat actatagatt caaattatag tagaaccgtt
    14641 tgtaataaag taagtgataa tataaaaaag tatcaattca acattggcaa agacaaaaac
    14701 atcatgaaat acgactataa ttttaaaaag tcactagatg aatacatatt tatattggat
    14761 aatttaaaaa aggcttatga tttaaaagaa agaataggta gaaaaagctt atgtaaaata
    14821 gctttagagg aaaatagatt tttgacagaa caacagatta gaaatatgct tttagaacta
    14881 caagactttg ggcttgtaga catacttgtt ggtcgtggag gttcaattat aacatcaaaa
    14941 ggagtagaat ttctaaaaaa tataaatagg tctaacaagt taaacagtta aacctattta
    15001 acctatttaa ttacattttc atatatttaa aaatttatta gaaataacct tttccacaaa
    15061 gttttaagta taattaatat ttttatctta tctatgctct gaaattactt aatattaaat
    15121 attaacttgt gaataaatat tttttattat caatattggc acgttgtttg cttaatatta
    15181 ttaacataaa attaaaatta ctggaggtat ttacaatgaa ttataatttt aatgagattg
    15241 ttgatagaag caataatttt tcatctaaat ggtcagaaat ggaaaaaaag tatggaacta
    15301 atgatttact tcctatgtgg gtggctgaca tggattttaa ggctgcacct tgtataattg
    15361 attctttaaa aaatagacta gaacaagaga tttatggcta cacaacaaga ccagattctt
    15421 acaatgaatc tatagtaaac tggctagata ggagacataa ctggaaaata aaaagtgagt
    15481 ggttaattta tagtccaggt gttatcccag ctataagtct tttaataaat gaactcacaa
    15541 aagcaaatga taaaataatg atacaagaac cagtttacag cccttttaat agtgttgtaa
    15601 aaaataacaa tagagaacta attataagcc cattacaaaa actagaaaat ggaaactaca
    15661 taatggatta tgaagacatt gaaaacaaga ttaaagatgt aaaattattc atactttgta
    15721 atcctcataa tccagttggt agagtatgga caaaagatga attaaaaaaa ctaggagata
    15781 tttgtcttaa gcataatgta aaaatcatat ctgatgaaat acatagtgac attatattaa
    15841 aaaaacataa gcatattcct atggcatcta tttctaaaga atttgaaaaa aatacaatta
    15901 cttgtatggc tccaactaaa acttttaata tagctggtct tcaatcatca tatgtagtac
    15961 tacctgatga aaaagactat aaattgttag atgatgcctt taccagaatt gatataaaga
    16021 gaaataattg ctttagctta gttgcaactg aagcttcata taataatggt gaatcttggc
    16081 tagaatcatt tttagaatat ttagaatcta atatagattt tgctataaaa tatataaatg
    16141 aaaatatgcc aaaacttaaa gttagaaaac cagaaggtac ttatttatta tgggtagact
    16201 ttagtgcact tggattaagt gatgaggaac tagaaagtat acttgtacaa aaaggtaaag
    16261 ttgctctaaa tcaaggaaac agctttggca ttggaggaag tgggtatcaa agaataaact
    16321 tagcttgtcc aagaagtatg cttgaagaag ctcttataag aataaaaaat gcaattaact
    16381 aaaaatattt aaggagaatt tgcactatga aaatgaatac agaaagaaaa ataccttcat
    16441 ttagatattc cttgttagta atgcttgcaa taatagccct aacatcagtt ggtatagttg
    16501 tatttaatgc ttctataact acaatgtttt tactttcatg gcttatagta gttcctgcag
    16561 ccatgaaact tggttatact aatgatgaaa tagaagcttt tggatttgaa gttggtaaag
    16621 atgcctttca atcaaactta attatacttt ctgttggtgt actaatagca gcttggatag
    16681 ctgctggtac aataccaaca gtagtatata gtggactaac aataataacc cctaaatatt
    16741 ttctgcttac aacattaatc gtatgttcat taacttctgt tgctactggt acatcttggg
    16801 ggactcttgg aacttctggt atagcaatga tgagtattgg tactagtatg ggtattcctc
    16861 ttggtttaac agctggtgca gtcatttctg gtgctttctt tggagataag atgtcacctc
    16921 tatcagattc tacaaactta gctgcagcag tttgtaagac agatgtaatt acacatatga
    16981 agcatatgtt ttataccaca ggacctgcat acgtaatatg tatagttctg tatacagtaa
    17041 ttggatttaa gtattcaaat aatactatag actatgttca aataaatcaa atcaaggatg
    17101 tattaaattc aaactttcat attggtttag tagcaatgat tccaataata tttttattat
    17161 tacttttatt gctacaaaag ccacccatta tatctatact aagttcagct atcatgggac
    17221 ttattatagc agtatttcaa gaaggagaaa aaataggtga tgttttaaat tacatgctat
    17281 caggatttac tatagatact ggatttgtct atgccgacaa actattaaat cgtggtggaa
    17341 taatgagtat ggctgaaacc gttttactag tatttgtagt tttcgttatt gctggtattc
    17401 tccaaaaaac tggattctta gaagtactgc ttcaacctct tataactaaa ataggaaaat
    17461 caagaactaa attggttggg tctactttta tagtaagcta ctttgcaaat gcatttagtt
    17521 catcaatgat gttcacttct gtatttgtcg gaactctaat gtctccaata tacaaagaat
    17581 ttaaattaaa accacaaaat ctatctagaa ttatagaaga tacagctaca cttggtggtc
    17641 cattaatacc atggaattca aatgctgttt tctgtgctca gactcttggt gtaagtccac
    17701 tagaatttat tccatattgt ttcttaagtt ggattacacc tataatttca tttatttatg
    17761 gtgtaactgg ttttactatg cttacataca cagatgatga aattgaagag ttagaaatat
    17821 ttgatactgc tcagagaaga gttgattgaa tatgaaattt gaatactatt acttaattca
    17881 ggatattgca ggtatattat tggcatttat aggacttaga atgtccatta ttggatttag
    17941 gatactttct atgagggggc tttctataaa tacactttta attgtaatta aatactgctt
    18001 atttaccata gcaggactga atttattaat atctaaattt ggaattagac actggatttg
    18061 gagtgtttgt atgttgataa tatccataat aataaatccc agaattaagg tatcaaaata
    18121 gaaatagtta ttagacttaa atttttataa aaaaactgct ttacaaatta aagcagtttt
    18181 tttattgtta cttataatta aataactggt gtatcagata agtaatcatt acttacataa
    18241 ccatatccac cattatactt tatttttacc catccagtag caaacatttc tattgtttct
    18301 actttgcttc catatgaaag gtgacctatc ttttcatctt ctgtacttgg tcctttccta
    18361 acatttagac catctttttc ttttacatac tttattttta tattttcatt actattatca
    18421 ggcttattat ttgtcaaata agacccacta acatatccaa taccttcatt gtattttatt
    18481 tttacccatc cagtttttaa cacatctaaa acttctactt catcaccatt atttagatat
    18541 cctataacag aagcttctat tgtagcatca tttcttatat ttaccttatc tgtattagtt
    18601 acatacatta ctctcttatt gttactattt gcctgtaaat cttttaattc tttaaatgca
    18661 ttttcatttc ctccactacc aacttgagtt atagaggtag catttttatt cgaaaataca
    18721 ctcttttgtg ttgcacttaa ttgttctcct acgattagta caggagaatt ttcttttgat
    18781 gctagtacac ctactgctag agcgtctata agttgatttt gatttttcat tccatcctta
    18841 gccacaaata cattgtttaa tttattagat gtataaaact tttctattac tttaccatta
    18901 gtttcatttc tatctgctcc acctattctt tctgaattag gtaaactttg ttttatttta
    18961 tctgatacag cattagtaga gcctattatg tatgatttat ttatattctt agattctata
    19021 aaatctttaa agctagatat accagatgca tcagatactg gaagtataac cattccatta
    19081 gtagcagcta cagaagctat actaactgca tctgcaagac ctgtgtaccc atttactaca
    19141 gctatttctg atatatcttt taatccttct atttttttag ctatactcaa agctgtttgg
    19201 tatctatcac taccaccaac tctatcagta gttaaattat tagattctaa ctgtttttgc
    19261 acatcatttg atacagaact agatagacca attatataaa cttttttaac tcctaatctt
    19321 tttaattctt tctcagtttg tgaatttaga ttattttttt ctgtcaataa tataggagca
    19381 ttttttaatt tagcaaatgg tgttacagct aatgcatcta caatagattc actatttaca
    19441 actacagcct catttgcact actccatcct tttttactta cttcaacagc agtttgatac
    19501 ctatcctgac ctactaaagt attctcaact gttgttctac ttatttcatt tgcatatgct
    19561 actggtgata agcttaaaac tagagataag cacagcattg atgatattga tttagttaat
    19621 ttcatcttga ctccccctaa aattatagta tctaaatgtt tcctatttaa aatagtataa
    19681 cataataatt taagtaacat cattattata aaatattatt tttcttatct aataagcttt
    19741 tattcttact tgacattaat actttgctca tcaaatgaat tttttatata actaaagttt
    19801 gtaacaatat ttccttagtt aatcatttaa agttactttt tttaattaga ttatgtacaa
    19861 aatataaata acaatttaaa taacttttta atatattgta gtttattata taaactttat
    19921 attataatgt ataatgtgga gttgattata aaattgatat aactcaagtc atattaaaag
    19981 aggtgtattt tatactatgg atttattgca tcttaagtat tttcaaactg ttgctagaat
    20041 ggagcacata acaaaagcat cacacaagtt aaatatagca cagccagctt taagcaaaac
    20101 tatatcttcc ttagaaaaag aacttggagt tcagttattt gacagaaaag gaagatatat
    20161 tgttttaaat gagtatggaa gattgtttct aaaaagagta gatagcattt tagatttagt
    20221 agaaagttca aaaaaggagt tacaagacac ttctttagaa aattcaggag aagtaaagat
    20281 attatctcct gcagcggcta atgtgttacc atcacttttg agtaatttca gaaaactata
    20341 tcccaatatt acttttaatg tgtcacatac tttgccatca tcttataaaa aaagtgattt
    20401 tgatttgtac atatcttctt catttacaaa gctaaactca gaaaatagca taactttaac
    20461 atgcgaagaa atactattag gagtttcaat aaaccatcct ttatcattaa aggatgaagt
    20521 atatctaagc gaagtttcag atgaaaactt tatagttata acgaagggtg agaattatag
    20581 agaggtaatt gatatcctgt gcgaatcagc taatttcaaa cccaaaatag catttgagtc
    20641 tgattcacca tatacaattt atgctcttat aaaatcactt caaggagttg gatttatatg
    20701 tggaaaatca tggggattat ctcaagaccc tgagattaag ttgttacata tcaaagatat
    20761 tgaatttaaa agatatttaa atctttcatg gtttagcgaa aactatgaat ctaaagctgt
    20821 gttgttgttc aaaaactttc ttatcaacta ttttaaaaat atttaaatca aagttgataa
    20881 tattaaagag gatatatcaa agtataataa atttaatctt aatattgata catcctcttt
    20941 ttatcatttt atattgaact tgtattatag tatagaatta tatgttatag ctactttttc
    21001 tgtctttcaa ataggggata tcttccctat acttttttat ttcatctaaa tcaatctctg
    21061 ataatacata tttttccctg tatgtatcta attgagttat tacagtacct ctaggattgc
    21121 atattttact ctttccaaat agtattaaat tagagtcatt acttactaaa tttacaccta
    21181 ctgtatatac agtgttctct aaagctcttt gagatacatt taaatcccac atatcttcat
    21241 cttgtattct ccatgcagat ggtataaata ttatttcaga accttttaat gtcaattctc
    21301 tggatacttc tggaaaacct gcatcataac atatcattac accaaatcta ccaaaatcag
    21361 catcatatac ctctactttt tcccctcctt taaagtatac tgcttctaat gaccacagat
    21421 gatttttaca gtattctccc ataatcttac cttttctatc aaatataact gctgaattat
    21481 aaacatgtgt agaccctttt tctaaggttc catatggaga tatcaggtat acattatttc
    21541 ttttagcagc ttctgacatt gattcctcta tgtacttatt atgttctctt attaattcta
    21601 atgttttaac tccacctaaa gactctaaat tatatcctgt agcaaatagt tctggtaaac
    21661 aaattatatc tgccccttgt tttcctaagt catctatcat ctctactgct ttttctatat
    21721 tttttttaac atttccaagt acactgtgtt gttgaattat acctattttc accttacgac
    21781 ccatgtaatt gcccccttta tttatctttg attccaaaac aattcattaa tatataataa
    21841 ataaccatac tcactacaat tccatttatc ggaggtatta agaaatttat ttttgaacta
    21901 taatacgacg ctgcaaaacc tattaaccaa cttattactg caatccaatt tattccttct
    21961 ctatgcttat aagttcttcc ttgtataaaa taatactcac accacataat tcctgctatt
    22021 gcaggaactg cgattcccaa gaaagataaa aatgactgaa atttaaatat tataccagta
    22081 gctgctatta ttgtacctat tagacctgca actaaagtca aaacatttct ttttacattt
    22141 ggaaaaatag tttttaatgc taatccacca gaataagcat ttgatacgtt tgtagaccaa
    22201 gatgcaagta ttaaaataac aaacgctacc catccaaaac ctaaattagc caatacttct
    22261 gttatatccc aagtaggtgc tcccatagac attacaccag ctgcaacttg ttggaataat
    22321 gaaataatcc ctagacctaa aaaactgata atccaaatat cttttactcg tctcgtatat
    22381 cttaactgat caggtgatat aattgcacca actataacaa gacctactac aatattaaga
    22441 ccttcaacca tactcatagt tgtttgagga gtataattat aaatagcttc aactccataa
    22501 gtagaaaaag ctttaaaaac tccccacacc attaatatca taagtggtgg tataactaag
    22561 taattaaatt tagccatagc tttaaagcca tatagtgcaa aaacaaccat aaataatcca
    22621 agtataactg taactaatgt taggtttaca cttgggaata tcttttgtac tgatagtcct
    22681 gcaacccctg cttgtacacc aaaccatcca aaactagtta taccaagtaa tagagatata
    22741 agatactttc ctcctatctc tccaaatgcc cccttagcta atacatatgt atttaaccct
    22801 tctttgtaac ctatataacc ttgtatcata ataattgcaa ctaatattaa gttccctata
    22861 attgatgtaa taatcatatt agaaagagtc attcctaaaa ttaaagttcc cccaaccatt
    22921 agttcagata tagctatgtt agaaccaagc atattaaaca atggagctaa ccatccaact
    22981 ctttcctctt ttggaactgg atttaaaact aattcttctc tctcttgcaa tattatccct
    23041 ccctttcttt ataaaagaat aatttctact tttttctact tttttttcac tattaataga
    23101 atttttcttt atataattgt aattctattc tacaaaaaag tataatatct agtttgttat
    23161 caatttgata taatttaagt tataaataga taaatagtcc aaatttatta ttttaattta
    23221 at
//
LOCUS       DAGSWI010000057        23081 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.057, whole genome shotgun sequence.
ACCESSION   DAGSWI010000057 DAGSWI010000000
VERSION     DAGSWI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23081
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.057"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(138..2210)
                     /locus_tag="MJF80_002053"
     CDS             complement(138..2210)
                     /locus_tag="MJF80_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBZ0280714.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGELLDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(2513..3355)
                     /locus_tag="MJF80_002054"
     CDS             complement(2513..3355)
                     /locus_tag="MJF80_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBZ0280715.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(3616..5658)
                     /locus_tag="MJF80_002055"
     CDS             complement(3616..5658)
                     /locus_tag="MJF80_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBZ0280716.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(5810..6781)
                     /locus_tag="MJF80_002056"
     CDS             complement(5810..6781)
                     /locus_tag="MJF80_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280717.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVLESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISINHFVACHLV"
     gene            complement(6782..7759)
                     /locus_tag="MJF80_002057"
     CDS             complement(6782..7759)
                     /locus_tag="MJF80_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280718.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVNFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            7978..9537
                     /locus_tag="MJF80_002058"
     CDS             7978..9537
                     /locus_tag="MJF80_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBZ0280719.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEEYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAKIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            9643..10602
                     /locus_tag="MJF80_002059"
     CDS             9643..10602
                     /locus_tag="MJF80_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280720.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            10605..11417
                     /locus_tag="MJF80_002060"
     CDS             10605..11417
                     /locus_tag="MJF80_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280721.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIITSFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(11633..12535)
                     /locus_tag="MJF80_002061"
     CDS             complement(11633..12535)
                     /locus_tag="MJF80_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBZ0280722.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIVFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            12890..14068
                     /locus_tag="MJF80_002062"
     CDS             12890..14068
                     /locus_tag="MJF80_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBZ0280723.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            14077..14733
                     /locus_tag="MJF80_002063"
     CDS             14077..14733
                     /locus_tag="MJF80_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBZ0280724.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            14733..15398
                     /locus_tag="MJF80_002064"
     CDS             14733..15398
                     /locus_tag="MJF80_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBZ0280725.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            15503..16279
                     /locus_tag="MJF80_002065"
     CDS             15503..16279
                     /locus_tag="MJF80_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBZ0280726.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            16341..17675
                     /locus_tag="MJF80_002066"
     CDS             16341..17675
                     /locus_tag="MJF80_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBZ0280727.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            18171..19016
                     /locus_tag="MJF80_002067"
     CDS             18171..19016
                     /locus_tag="MJF80_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBZ0280728.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPESNKPEDNNNSDSTNKPDDNNNSGSTSESFSAYQK
                     EVVDLVNIERAKAGLNPLTLDSSISNVATKKSQDMIDNNYFSHNSPTYGSPFDMLKKF
                     GISYKTAGENIAMGQKTPKEVVNAWMNSEGHRKNIMNPNFSKIGVGVAQKSGGSIYWT
                     QIFVG"
     gene            complement(19408..22947)
                     /gene="nifJ"
                     /locus_tag="MJF80_002068"
     CDS             complement(19408..22947)
                     /gene="nifJ"
                     /locus_tag="MJF80_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBZ0280729.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPVNT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMGICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         7943 a         3701 c         3161 g         8276 t
ORIGIN      
        1 aatgttttaa aatatatcaa aatataatga tatatatttc tattacttaa aatatatatt
       61 aaataaattg catgttaact gaacctattt atatatttat taaatctcac ataactttta
      121 gttacactat catatagcta tcctattttt tcaatatcat ctcttaatat acttactcta
      181 ttctcctctt ttgctgtggt tactcctttt ttaatgctta atttctgatt ttgttctaaa
      241 ttcgtaaata ctacaggact tattgttgat agaccatttt tttcaatata ttctaagtcc
      301 attttaacta atttttctcc cgcttttact tcatctccta cattcacaaa tacttcaaat
      361 ccatttcctt ctaagtttac agtttctatc cctaaatgta taagtatttc tactccactt
      421 ttttgggatt ttattattat tgcatgctta gttggaaata taactccaat agttccatca
      481 actggtgaat aaatcaatcc atcactagat ttaattgcaa atccatcccc catcagttta
      541 cttgaaaata cagaatctgg cacttcttct atatctaata actctccatt tgttaatgaa
      601 tcaatctttt ctactaaagt attttccata tttgcaactt ccatattttt tttcacttta
      661 tttttatcaa catttttagt aacagtactt tcaacctttg tttttccctc tattatatct
      721 tttatatcag actttattga atctgatttt ggtccaaata tagcttgaac attgttgcca
      781 ataaccataa cacctgctgc tccaagtgat ttcaattttt ctttatctac tttatttata
      841 tcatttacaa ccaatctaag tcttgttata cacgcatcta gactctttaa gttgtcttta
      901 tttcctaaag caatcaaaat ctctgatgct aattcattct tagacatttc aatgacttca
      961 gcgttagttt ctgactcatc ttctcttcct ggtgtcttta aattaaattt tcttatcata
     1021 aatctaaata cactatagta taatactgca aaaccaatac ctaccaagat agccaagtac
     1081 catggagttc tattaggaag aacaccaaat aataagaaat ctattaatcc tcctgaaaat
     1141 gtgaatccaa ttttaactcc taataattgc ataattgcgt atgataaacc tgaaaataaa
     1201 caatgtattg caaataatac tggtgcaaca aataaaaacg caaactctat tggctcggtt
     1261 attcctgtta aaaaagaggt taatgctgct gatgccatta tacctgctac aaattttttc
     1321 ttttctggct ttgcttcatg caccatagca agagctgctg ctggaagacc aaacatcata
     1381 aatggaaatg cacctgtcat aaaagtacct gctgtgaaat ttactccatc tttaagttga
     1441 gcaaagaata tcttttggtc acccattaca agctcaccag ctttatttac atattctcca
     1501 aattgatacc aaaatggatt atggaaaaca tgatgcaaac caaatggtat taatagtctt
     1561 tccaaagaac cttccataaa tgctgctagt gtttgatttg cttctataac attttttgag
     1621 aaactaagta atccctcttg tattggaggc caaataaaag acaatataat tcctattgct
     1681 atagcaacta atgaagtaac gattggtaca aatcttttac ctgcaaaaaa acctagatac
     1741 tgaggtaatt caattttata aaacttttta taaagataag cagccactat ccctataata
     1801 ataccaccaa atacccctgt ttgaattgtt ggtattccaa caatatttgc atacatagaa
     1861 ttgtctacca tatcttctgt aacacctgcc ataacaccca tagatgtatt gagaattaaa
     1921 aaacctacta tggctgccaa tgctgcaaca ccttctcctt cagttaaacc aacagctacc
     1981 ccaactgcaa atataagtgg taagttatta aatataattt gacctgaact ttccattata
     2041 cttattataa gttgaaatgt aggattcgcc aaagctggaa aatgagccaa caatgcgtct
     2101 cctgctaaag cattactcac gccaagtaat atacctgctg caggaagtaa tgcaactggt
     2161 agcattagag attttcctac tttttgtaaa acaccaaata cttttttcat ttacttttct
     2221 ctccttatta ttttatattt ttttaaataa aaaagactta agtttataaa aagttacatt
     2281 agtatactta attctaaaat taaacttatg ttaatataaa caaacctata ttaacctaat
     2341 ttcagaatta aaaataaact attataattt ttttataaac ataagtcttg cctgcattat
     2401 cagtaacaat cttaatgttt taaaattatt attttatatt aaattttaaa taatatatta
     2461 aaattaaaca taatatatta aatctataca ttctgtatta aactactctt tattataaat
     2521 tcagtacaga tataactctt ttgatatgta atgttaaata aagtagttca tcttgactta
     2581 catagtaatc ataattcttt tctacatgag tttttatttt atatgcacaa tcataatcct
     2641 tttcatactt tactttaatt atatctataa tttcattatt tgtatcattg attttttctt
     2701 tttttactaa tcgttttgca aagaatttta aatgagtcag aagcctatca taatttatat
     2761 catcttcttt aaattcaact ctataataat atcttataat atttagtatg tcttttacaa
     2821 ttttagtaat taacagtgat tcctttgaac ttcccttata gtttgaattt actatatgca
     2881 ttgctataaa accagcttca tctactggaa atttcacatc aaattcttta tttatataat
     2941 ctacagccca tttcccaatc tcatattctt ttttatgtat tctttttatc tcatgtagta
     3001 aatcattctt tatagttatg ttttcatgat atcttttaat tgcaaatgca atatggtcag
     3061 taagtgctac ataaatatat tcataaagtt tagtattcaa attctcctct gcataagcaa
     3121 ttatttcagt actaacctca aaaaatttct catcaacctg gcttgctagt ttactaattt
     3181 tatctaaaaa gctttcatct tgtattacaa aagttctttc aattttactt ttatctattt
     3241 cttgaccttt ctttttttga aacccaattc cacatcctgt taatattaat tcttttttta
     3301 tattttcatc taaaactaat acaacattat tattataaat ttgttgaata atcataatca
     3361 atatctctcc caaaatacaa tttcccttat tcataaaaaa atacctaaat taagatacaa
     3421 ttcacctgta taccttaacc taggtattgc ctgcttacca gtaacaatcc agcttgttaa
     3481 cgcttatata ttgtattcaa tttatggttt tattctatta taaataaata tatatgtcaa
     3541 gtaaattatt gaaaactatt tcctattttt tagtattaat tttaacatca aaaatcaaat
     3601 tttattccat tatatttatg attctatcat catcatttca tcatagagaa tattttccaa
     3661 taactcttta gccacttctc ttgatatttg cttagtttca actaattgtt gaacactgtc
     3721 tctttgactt tgtatcgcca ttactctgta gtattctact cttgcctcaa acttatcatc
     3781 attttgaatc tttctagtaa tccaattcaa ataacgatta tagaatctga ttgctattaa
     3841 ggcctcatgt ctgttatctt catcagtatt tttatttata aatgatatag cagttttagt
     3901 agaaagtgca tatgcatggt gaaactcttt gattatttct ttattctcag atactatttt
     3961 tgtaattttt tcttttactt ctttctttac ttttctattt ataatccaca tgcttatttt
     4021 taattttaca attttaaata tagatttttg aacagatttt cttgattttg taatataaaa
     4081 tctatacaat ttaaaggctc tatcagaaat tttaccatct tctaatagtt tcaaaacagc
     4141 taataatccc tgttggtctg cttctgcaat catcatactt ataagtttat tatctggagt
     4201 atttagtatt cctttttctg agtctaccaa ctgttcattt aaatcatgta taactggttg
     4261 tacttcttgt gctttttcat tactaagttt taaaatggct tctttcaaaa ttagtttata
     4321 tgcctcttta tcagataata aaggttctga ctcttgtacg ttatttttta ataatcttgg
     4381 cagtaaaact gttgccaata ctaaacttat aagaataact tctgatgata taaataaaac
     4441 agtatctctc attggaaata catctttatc agccaaataa aaaggtatag ataaggcagt
     4501 tgctagtgta aatgtaccat gaacaccaca agtagcaact attagtgagt atttacattt
     4561 agaaatactc tcttttacaa cttcttcatc cttaaaacct aaaaaaccta caaaaaaatt
     4621 atttaaagga ttctttttag gttttataaa agaattatgc cataaatata cccatataaa
     4681 tcttataaca aataaaataa gtacaattaa agtagaaata aaagtcaatt ccaatgccat
     4741 attttcattt ttactactaa gtcctgtata tatacttggt aacaaaaaac caagcagtgt
     4801 aaagacaaat ccattcaata catactccaa aacataccaa gtattgtctg atattatcct
     4861 taatttagta gttgtatttt gtaaatgctc tttttcaatt ccatgagcta tccctgccat
     4921 tacaacagct aggattccag atactcctat ttcttctgct acaaaatata caaaaagtgg
     4981 agtcattatt tgtataacta ttaccattgg aatttcttcc aaattccact tgtgtaaact
     5041 aagtcttatt tttataatta tgtatcctaa aatacttcct actattattc ctccaaaagc
     5101 tgtaatcata aattgaatcc ctgcctcttc tattgaaaaa actcctgtaa ctgtggcaag
     5161 tactgcaacc ttaaatgcta ctactccagc agcatcattt aataaagatt caccttcaag
     5221 tatactcatc atatttttag gaaaatttaa tccttgtgta attgatttta ctgctactaa
     5281 atcagttgga gatattactg ctgccaaagc aaatgaaaca gataatggca tcccaggaat
     5341 aagaaaatgt ataaagattc cacctgcaaa cacggttatt aaaactaaac caaatgcaag
     5401 aagcaaaatt ggttttctga gctctaatag ctcttttctc gatacatttt gtccttcact
     5461 gaatagtaat ggagctatga tacatattac aaatatttct ggattaaatg aaaatgagaa
     5521 tttaaatggc aaaatagaca atataatccc tagtacaatt tgatatattg caagaggaat
     5581 attactccac tttttgctaa atacagttga aatcgttgta caaaccaata atcccaacaa
     5641 tgttactaaa attgacataa ctacctctct tttcaataat aatattatag attaaactta
     5701 tatatcaact aacttaatat taacagcatg aaatcccatc ttaatcaaaa aaattttcag
     5761 tttattctat tattcttatt atatacatca aatttaattt ttgaaatatc tatactagat
     5821 gacatgcaac aaaatgattt attgatattt cttttaaaac tggaatctct tttttgcata
     5881 tttccttaca ataagggcat cttgtatgaa acttacatcc acttggtgga tttgttggag
     5941 atggtatgtc tccttttaat ataattctat ttttcttaat tgtagggtct tgtattggta
     6001 ctgcgctcat aagcgctttt gtatatggat gttgagaatg taagtaaagt tctcctttag
     6061 ttgcaatttc tattatagaa cctagataca taacacaaac tctatgtgca atatgattta
     6121 ctacactcaa atcatgagat ataaataaat atgaaagttt atatttatcc tgtaaatcca
     6181 taagtaaatt tataatttga gattgaatag aaacatctag tgcagaaaca ggttcatcac
     6241 aaactataaa ttctggattt aatgctaatg ctcttgctat acctattctc tgtctttgac
     6301 ctccagaaaa ctcacctggt tttctattta aatggtactt agaaagacca caactttcta
     6361 aaacatttaa tatattatct tttatttcat tctttggcac taaattatgt tctataactg
     6421 cctcggatat tatttgttcc acatttaatc ttggatttaa tgaactatat gggtcttgaa
     6481 atatcatttg cattttgggt cttaactttc taagttcttc cttctctaac ttatatatat
     6541 ctacaccatt aaattcaact ttaccttcag ttttttcaat taatctaaga atggttcttc
     6601 ctgcagttga ttttccacat cccgattctc caactatagc caaagtttct ccttttttta
     6661 aatcaaaaga aattccatct actgccttta cacatttaat ttccttatca aataatccag
     6721 atttaacttc aaagtatttt ttaagaccct ctacttttaa taaactctct tcttttttca
     6781 attatatctc ctccttttta tataaccaac atgcaatact atgaccacta ttcaattcta
     6841 ttaaaggagg tatctgagtt ctacatttat cacatacttt ttcacatcta tcactaaaat
     6901 aacatgaatc tggcatccca attggatttg gtacttgtcc tggaatcgaa tacaatcttt
     6961 tatctttgtt ttttccaaga gcaggctttg attttagaag tccaatagta taaggatgtt
     7021 ttggattttt aaataattca acaacctctg ccacttctat aattttgcca gcatacatca
     7081 ctaatacttt gtcacacatt tctgcaacta ctcccaaatc atgtgtaatc atcattatag
     7141 atgtgtttaa tttttctttt atatttttca ttatatctaa aatctgagct tgaatagtaa
     7201 catctaaagc tgttgttggt tcatcagcta taagtaattt tggattacat gataaagcca
     7261 tagctatcat tattcgttgt ctcataccac ctgataattc atgtggatat gaattatata
     7321 tttcttcagc tcttggtatt tctaccagtt ttatcatatc gattaccttt ttcttagctt
     7381 cttcctttga taaatcttga tgtatcataa taccttcaat gatttgactt ccaatagtaa
     7441 acgctgggtt taaagatgtc attggttctt gaaaaatcat tgatatctca ttccctctta
     7501 ttcccataag ttcatcttca ttcatcttta aaatatcttt gcctcgaaag attatttctc
     7561 caccttctat ttttgatgtt ggaagtaact tcatcaaaga catggctgtt acagacttac
     7621 cacatcctga ttctccaact attccaatag tttcaccttc atttatatta aaatttactt
     7681 catttacact ctttacaatc cctttatctg tatgaaaata aacttttaaa tctttaacct
     7741 caattagtgc tttactcatt attttctcct ctcagcaaaa ttacttaaga taacatacac
     7801 cttttgattg tattttataa aaatttaaat tacaacattt tcttacattt tattattttt
     7861 ttctattaat tataataata taatagttaa tttctatata tattgattta ataatgatta
     7921 ctattataca ataaaaaata tgtgttaaat attattttac ggaggtttta taagaatatg
     7981 aaatttaaaa aattggcatc attaatctta gtttcatcat taatgcttac atttacagcc
     8041 tgtgcttcca atgataaaga caagtctagt ggaagcaaat ctggtggaac tgttgtaata
     8101 cctatggcaa gtgacccaga tataatcaat ggagcttttg caaacgttaa agaagactct
     8161 cttgcatcaa atatagttta tgctcctctt tatacttatg aaaaaggaaa tttagtaaat
     8221 tatttagcag aaaaagtaga ttttaaagat agtaaggaat taactataaa attaaaatcc
     8281 aatttaaaat ggcatgatgg aaagccaata actgctgaag atgtcttatt tacatttaat
     8341 acagttttag acgaaaaaca aaattcacca tcaagacaat acttattggt gggtgagaaa
     8401 ccagttaaag tagaaaaaat tgatgattta acagttaaga ttactttacc aactgcaagt
     8461 gaatctttct tatatggtat atctaaaatt tctccaatac caaaacatgt gtttgaagga
     8521 gaatcaaata tagctaagtc agaaaaaaat aataatccag ttggttctgg tgcattcaag
     8581 tttaaggaat ggaaaaaagg tgaaagcata gtatttgaaa agaatgcaga ttattttggt
     8641 ggagaaccta aggcagattc tatagcgcta aaaataattc ctaatgaagc atctcaagaa
     8701 gcagctttaa ataatggcga aatttcactt atgaagacat ctgctgaagg atatgaaaag
     8761 gcaaaatcaa actcaaactt acaaacttat acttatagtg aagaaagatt aaactacatt
     8821 gtatttaacc aaaatatatc aaacatggct aacaaagaag taagacaagc cttatcatat
     8881 gccttaaata gaaatgaaat gatagaatca gcatatggaa aagaaggttc tgtacctgct
     8941 aaatctatat tagttccaga agcagacttc tatacagaag aaggtgttga agaatatgac
     9001 caagatacaa ataaggctaa agacttattg gataaaagtg gtgttaaaat tgataaatta
     9061 aaaataggtt ataacacagg tagatttggg cataaaaact atgccttagt agctcaacaa
     9121 gagttgaaaa aaattggtat agaagctgaa atagttcctt atgaaagtaa ggcattcttc
     9181 aatatactat ttagtaatag cactgaatgt gatatgtatg taaatggtta tgcttgggga
     9241 ttagaaccaa acccatatag aggtatgttt gaaactggtc aatattgcaa ccaaactaaa
     9301 tattctaatg ctaaaataga tgctttatgg gaaaaaggat ttactgagtt aaacaaggaa
     9361 aaacgtgaag aaatatataa gcaaatacaa caggatatat ctaaggatgc gcctatttac
     9421 actatagatt atgaacaaaa tttaatggca gctcaaaaaa atctaaaagg aataaaagat
     9481 gctaaaccat ctcctgcaat attatttgaa gattggtcta aactgtatgt agaataattg
     9541 atgtaaaatt ttatattatt aattagaaaa ggagatgtct aaaaataata ttgttttatt
     9601 attttaaaac gagacatctc ttttaattag gagaatttac ttatgttaaa attaatattg
     9661 aatagagtaa aagttttaat acctatgtta atcttaatat ctatattatc atttggactt
     9721 ttagaattag ctcctggtga cccagcagat gcatatataa atcctttaat gtcttcacag
     9781 gatattgaaa atataagggt taatatgggg cttgataaac cagtatatat aagatattta
     9841 aactggttga aaaacacttt aagtggaaac ttaggtattt cttatataaa ccatatgccc
     9901 gttacagaac aaattatgga aaaaatggga aatacattta ttcttatggg tacttctttg
     9961 atattttcaa tattggtagc tatacctctt ggaatatttt tagctgtaaa taaaaattca
    10021 ataactagta aagtatcatc tgtttttaat tatataggtg tttctatacc ttctttttgg
    10081 ataggtatga tactcatttc aattttttca gttaaactta acattttccc atcaggaggt
    10141 atgcatacta ttggaaatga tagtattgga gatttagtaa aacatttagt tttgcctgta
    10201 ataactttag gtctatataa tactgctatt tttacaaatt atgtagaagc tagtgtaaat
    10261 gaacaattaa aaaaacaata tgttattaca gcaagagcaa aaggattatc agaaaaagtc
    10321 atactattta aacatgtctt gaaaaattca ctgacatcat tggttacaat cctaggcatg
    10381 agtatacaaa aattagtcac tggtgcattt gttacagaag tagttttctc ttggccaggt
    10441 atgggaagat taatgattga ttcaattttt tcaagagatt atactgtaat tatggcaata
    10501 actatgttat cagcactatt tttaatttta ggtaatttag ttgctgatat actatactta
    10561 ttaattgacc ctaaaataaa atctagcaaa ggaggattct agttgtgttt acaagtatct
    10621 taaaaaaatt aaaaaaagat aaatcctttg cttttgcatg tattactctt agcataataa
    10681 tcatattaat tataacttct tttatattaa atatagactc taatgcaatt gatactactt
    10741 ctaaacttca agaacctagc ttaaagcata tctttggtac agacgaactt ggtagagatt
    10801 acttctctag agctctttat ggtggaagga tttcacttag tgttggtatt ttatcaatgc
    10861 ttatttctgt agtatttggt actgctgtag gtgttattag tggatatatt ggaggtaagc
    10921 tagaccaatt cttgatgaga ttagtagata tgcttatgag tatacccagt ttcttagttt
    10981 tgattgtatt aaatacttat ctaacaccta aagtttccac tattatacta attattggat
    11041 tcttaagttg gatggaagtt tcaagaattg tacgtggcga aacattaaaa ataaaggaaa
    11101 atgaatactg tctagctgct aaagctactg gtatcaagac tagaaatata ctattaagac
    11161 atatagtttt aaacttaaaa gagactattg tagttgctgg ttcgttaaat attgctaatg
    11221 ctatcttaac tgaatctgct cttagttttt taggtcttgg aatccaattg cctcttgcat
    11281 catggggtaa tatgcttcaa gatgctcaaa gacatatttt tgacaagtta tatttagctg
    11341 tatttccagg attacttata tttttaacag tcatgagttt gactataata agcaaaaaat
    11401 tttctagtaa tacttaacaa attttatata gaaaataaaa acagtgatta tctaaagata
    11461 tacactgttt ttatttttcg tttaaaaaaa tacatatttt gtatattata actttaaata
    11521 ctactctacc atgttatgca aagcgtatta ttgctctcaa acatatagat gaaaaactat
    11581 tagtttatta taataaaaat tgatttattt atttgttatt ttaataacat ttttaagagt
    11641 tctttttact aaagaaatct ataataaatt taaatacatg ttggtctaca tggctataac
    11701 gttctttttt aggtctacat aatataactt tccatgtaat aggatgatta aaaggctttt
    11761 ctataatttt gctattttca aaaaaatcat gaactggtga tggtaaaatt gttataaaat
    11821 cagaatttgt ggttgatagc aacaaaaaat cccatgaacc agacattata tatcttttta
    11881 acttaacatt ttcatcagta aatttttgca ttagcttatg atgtatcata aaagtaggat
    11941 taaatattgc aagtaactgc tcattcaaat cgttccaatc tatcttatcc ttcttagcaa
    12001 gtggatgatt tatatttaaa aatgctgtaa gttcatcttc ttgaagcaca tgttcagtta
    12061 ctacactttt atcaatttta tgtggatgta acaatactgc ataatctaat tcctgtaaga
    12121 taagcattct atttaagtca tatgcaccct tctctactat atcaaattct atatctggat
    12181 tatttgcaac taactgtgca actacatctg aaaatactat tccaagtata agtggaggta
    12241 ttcctattct aacttttcct ttaaattgaa ctgaatcttc tcttaaatct tccatcatat
    12301 ttttgtattc atttatgata tgttctgcat ttacataaaa tctttctcct gctggtgtta
    12361 acccatttaa cctgccttta tacctttcaa ataaatatac atcttcactt tcttcaaaat
    12421 tttttataat ttgacttaat gctggttgag atatgtgaag tgtcttagat gctattgata
    12481 aattaaaacc agatttcact atttcaacaa aatatttcaa ctgttttata tccattttaa
    12541 acccccttcc ccaaaagtat aatttagatt atattttttc ttctaatgat attttatagg
    12601 aaaatcaaca taatttcatt aatattttaa caaaatattt ttctaaatct actataaatt
    12661 tttatcctat aaatttttct tatacactat ataaaaaatt agacttagac aaaacgcttt
    12721 tttcgccgta atctataatt agaaacagga aaacctaaag cattaggaat aaaagtaatt
    12781 gaaatactta aaaacaagct tgtattaaaa aagtacttta ttatagtgtt ccctaatttc
    12841 tttattcaga aaatttttaa ttatcattca agaaaggtaa ctattagata tgaaagatgt
    12901 agtaatcgta tcagcagtca gaacacctat aggaagtttt ggaggtgtat ttaaaaatac
    12961 ttcagctgtt caacttggaa ctatcgctgt taaagaagcg atatcacgtg taggacttaa
    13021 tttatctgaa atagatgaag taataattgg aaatgttctt caaacagggc ttggacagaa
    13081 tgttgctaga caaattgcta taaatgcagg tataccaaat tctgttccaa gctacacagt
    13141 aaataaatta tgtggttcag ggcttaaaag tgtccagcta gctgcccaat caatcacatc
    13201 tggtgaaaat gatgtggtaa ttgcaggtgg tactgaaaat atgagccaag ctccttatat
    13261 cgttccaact gctcgctttg gtagtaaaat gggtgacata accatggtag atagtatgct
    13321 aacagatggc cttattgatg ctttcaatca gtatcatatg ggaataactg cagaaaatat
    13381 tgcaactaaa tttgaattta ctagagaaat gcaagataaa ttggcattag aaagtcaaaa
    13441 taaggctgag aatgctataa aaaacaatcg ctttaaagaa gaaatcgttc cagtagatgt
    13501 tttaatacgt cgaggaaaaa tagaaactat tgataaagat gaatatccta aattaggtat
    13561 gacatttgaa ggtttagcta aacttaaacc tgcattcaaa aaagatggta ctgtaacagc
    13621 tggaaatgcc tcaggtatta atgatggtgc ggctatgtta atactaatga gccaacaaaa
    13681 agcagacgaa ctaggtatta gaccacttgc taaaataaaa tcctatgctt cagctggtgt
    13741 tgaacctgaa gtcatgggta ctggtccaat ccctgcaact agaaaagcac tgaaaaaagc
    13801 tggacttagc atcaatgata tagacttaat agaagcaaac gaagcgtttg ctgcccaagc
    13861 attagctgtt aaaaatgaac ttcaaataga ttcatcaaag ttaaatgtta atggtggtgc
    13921 aatagcatta ggacatccaa taggagcaag tggagctaga atacttgtta ccttaattta
    13981 tgaaatgcaa aaacgtaaag ttgaaactgg acttgcaaca ctttgtattg gtggtggtca
    14041 aggaatatca atggtggttt cacgatagga ggaaatatga ataaaatagt tagcattgat
    14101 gaagctctat ctcatgtaaa agatggagat acaataatgg ttggtggatt catggccaat
    14161 ggttcaccag aaaatttaat tgattattta tgtagtaaaa atataaaaga tttgacacta
    14221 atttgcaatg atactggctt tattgacaaa ggagttggca aaatggttgt aaataagcaa
    14281 tttaaaaaga taattgcttc tcatgtagga ttaaataaag aaactggaag acaaatgaac
    14341 aacaatgaaa cagatgttga gttagttcct cagggaactc ttgctgaaca aatacgtgca
    14401 gctggttatg gtctaggagg tatactcaca ccgactggta ttggtactct tgttgaggag
    14461 ggaaagcaaa aaattgttgt cgatagcaaa gaataccttc ttgaaaaacc aatttatgca
    14521 gatgttgctc ttctatttgc atcaaaggtc gataaagcag gtaatctagt ttataaaggc
    14581 tctatgaaca attttaataa tttaatggca tctgctgcca aaattacaat agttgaagca
    14641 gatgaaatcg ttgaaattgg tgatattgac ccaaatgaag ttaatacacc aggtattttt
    14701 gtgaattaca tcgttgaagg aggtaattta taatggataa attagaaatg caagaatata
    14761 ttgcaaatag agtatctaaa gaattaaaag acggagccgt tgtaaacctt ggaataggtc
    14821 taccaaccaa agtagcaaat tatattccgg ataatgtaaa cgttatctta caatctgaaa
    14881 atggcttttt aggtttaggt tcagccgaag atggaaattc aagtgatgaa actattgtaa
    14941 atgcaggtgg tcaaccagtt actatacttc ctggaggatg tttctttgat agtgctactt
    15001 cttttggaat aattagagga ggtcatgttg atataactgt tcttggcgca cttcaggttg
    15061 acaaatatgg caatatagca aactatatga ttccaggtaa gatggttcca ggtatgggtg
    15121 gagctatgga cctagtaact ggagctaaaa aagttattgt agcaatggaa catacttcaa
    15181 aaggttctgc aaaaatatta aataattgta ctcttccact tactgcaaca aatgctgtag
    15241 atttgatagt tactgaaatg ggagttatgg aagtaacatc agatggaatt ttacttaaag
    15301 aaataaatcc agctttcaca ttagatgatg ttatttctgc tactgaagct cctttaatat
    15361 tgtcagattc attaaatgga aaaatatctg ttgtttaaaa taattaaaac tagaaaataa
    15421 aaatctctta gacgtattac atataaattt gtattgctta aaatattaat ataaaaataa
    15481 aataaaattg gaggaaatga aaatggttaa agataaagtt gtattcgtaa caggagctgc
    15541 aagtggaata ggtaaacaaa tagctgaatc gttcttaaaa aatggtgcta aagtaatgtt
    15601 ctctgatata aatcaagaag ctttaaataa taccataaca gagttacaaa aaaaaggtta
    15661 tgattgcatg agtgtaaagt gtgatgtaac taaggaagaa gaaattaacc acgctataga
    15721 caaaactgtt gaaaagtatg gcagattaga tatattaata aacaatgctg gacttcaaca
    15781 tgtttctatg atagaagatt tcccaactga taaatttgaa tttatgataa aaataatgct
    15841 gacagcacct tttatagcta ctaaaaaagc atttcctata atgaaaaaac aaggctttgg
    15901 tcgtataata aatatggcct caatcaatgg tgtaatagga tttgctggaa aggctgctta
    15961 taactcagca aaacatggac ttattggact aacaaaagtt gctgctcttg aagctgccga
    16021 ttctggtata acagttaatg ctttatgccc tggatatgta gatactccac ttgtcagagg
    16081 ccagttcaat gacctttcaa aaactcgtaa tataccattg gaaaatgtct tagaggaagt
    16141 tttataccca cttgtaccac aaaaacgcct attagatgtt caagaaattg ccgactatgc
    16201 tatgttctta gcaagtgata atgctaaggg tattacagga caagcttgct tactagatgg
    16261 aggatatact gcacaatagt tattatattt agggcttgaa gaaagtattc ttcaggtcct
    16321 tctactaagg agggatattt atgagtatta tcgcgccaat tggtataatt attggtatca
    16381 tagctattat atatttttca gttaaagaaa ttcatataac tatagctgct ccaatagcaa
    16441 ctttaatagt tgttatttta aataaaatgg atgttgtaac atcaatgcta ggtgctgaca
    16501 aaaacaatta tatgggtgca cttggaaact atataatgag ttattttgca atattcttat
    16561 taggctcaat acttgctaaa tttatggaag aaagtggagc tactgtatca atagctgatt
    16621 ttattttaaa taaatttggc tctaatcatc cttatagagt tttagttgct atatttatag
    16681 taagttttat attaacgtat ggtggtataa gcttatttgt agttatgttt gcggtcattc
    16741 cacttgcaag aactctattt aaaaaacttg atatttcttg gaaccttatt cagataccac
    16801 tttggcttgg tattgcaaca attactatga ctataattcc tggaacacca gccattcaaa
    16861 atgttatacc tattcaatat ctaaatacgt ctcttactgc agctactata ccaagtattg
    16921 taggtagtat aggttgtgct acgtttggac ttgtctatat gaaatatgct ctaaacaaaa
    16981 gtattagaaa aggtgaaact tatgcaactt atacttcaga attagaagaa gaatctttag
    17041 atagagaatt accaggattt tttgcaagta tttttcctct attagcactc gtaattatag
    17101 ctataactgg aagtactttt ggttcagaat ttatgaaaaa aaatgtaatt tacatagctt
    17161 taatagttgc aattctttta tctgcttttt tattccataa atttctatca tctaagatac
    17221 agacattgag cataggtgct agtggttcta taggtcctat atttgctaca tcttctgcag
    17281 ttgcatttgg agcagtaata atggctgcac ctggttttga tgtatttagc aaacttataa
    17341 tgtcaatacc aggtagtcca cttataagtc ttactgtttt gacttcaaca atgtcagcaa
    17401 taacagggtc ctcttctggt gcattaggaa tagtaatgcc aaactttgca gattattatt
    17461 taagtactgg tctaaatcca gaattaattc accgtgttgc tacaatagct tcaaatatac
    17521 taactatagt tccacaaagt ggtgtttttc ttacattctt aagcttatct aagttaacac
    17581 ataaaactgg atttaaagaa actttcattg tagttgcagt tggttcacta atagcagaaa
    17641 ttattgtaat tacattagga ttactaatgt attaatataa aacaaattaa aattcataat
    17701 atgaaaggtg gtgtctcaaa gtgaactttt tacttaatga gacactcttt ttatatgaaa
    17761 taacatatgc ctatcatttc aacaataaaa aaggccttat ctctattttg agacagaccc
    17821 tttttatttc ttaaagattt aaataattat aatttgttgc taatacgaaa gcatctactg
    17881 aataaattta accaaataaa aaaaaattta tcataactat aaatttacgt tatggaatta
    17941 ttataaaaat attattttct ctaactaagt aattccatac tatacaggct tttaatacaa
    18001 attgtcaaac acactgaaat atacctattt attccatata tatctttttt acatatcttt
    18061 acagtaaaaa aagtcttaat cttatgttag tatagattca cgggtaaccc cctacactaa
    18121 aggttacaaa tcagtaacat ttttagaatt tttagattaa gaggtgttta atgatgagaa
    18181 gaaatatacc aacaaaatta aaaactatat tagctttagg agtgttaact ttagcaacag
    18241 cttcaagtgc tgttactgca agtgcttcat cacttgaaaa taatgtaaat ataagtaaca
    18301 aaagtttagt atatagtaat actaaataca agttttctgc aaaaccaaat tgttctgtaa
    18361 aaccagaact taagcctgat aaagataact cttgtaagga taagaatcat aatgacaaga
    18421 attgcaacaa cactaataaa cctgaggaca acaataattc agactctact aataagcctg
    18481 ataacaataa acctgagtct aataaacctg aagacaacaa taattcaggc tctactaata
    18541 aacctgagtc taataaacct gaagacaaca ataattcaga ctctactaac aaacctgatg
    18601 ataataacaa ctcaggctct acaagtgaaa gtttttcagc ttaccaaaaa gaagttgttg
    18661 acttagtaaa tatagaaaga gctaaagctg gattaaatcc tcttactctt gattcttcta
    18721 tatcaaatgt tgctacaaaa aaatctcaag atatgattga taacaactat ttttcacaca
    18781 attctccaac ttatggttct ccatttgaca tgttgaaaaa atttggaata agctataaga
    18841 cagcaggtga aaatatagct atgggtcaaa aaactcctaa agaagttgta aatgcatgga
    18901 tgaattctga aggtcacaga aaaaatataa tgaatccaaa tttcagtaaa ataggtgttg
    18961 gagtagcaca aaaaagtggt ggttcaattt attggacaca aatctttgta ggataaaata
    19021 ctctttttcc atacatatca aaagagtatc tctaagtgat aaaaaacaca aatgagatac
    19081 tcttttgtat ctattttaaa attatcaaat ccaaatatac atactataaa tattatttgt
    19141 tctcttaatt ttagtgtgta gagcaatata taaaaagtat tggcaagatg aaaatggaaa
    19201 acttaaaaag atatttaata ggtaaaaaca ggatcttaat atcttcataa aaaaaggctg
    19261 tctcaaaata gagataaggc ctttttcatt gttgaaatga ttaaaatata tttgatttca
    19321 tacaaaaaga gtgcctcatg aactaaaaag ctcactttga gacactctct tttatcaatt
    19381 ctatataagc attataatac tttaatatta ttcagctaat tttttgtatc cttcatatct
    19441 ttctttagca tcagcttcag ctttagcaaa taatccatca gcttcctctg ggaattgttt
    19501 agctatagca gaatatctta cttgtgcaag tatgaaatct ttgaagcttg cagttggttc
    19561 ttttgaatct aaagtgaatg ggtttttacc ttcagctttt acttctggat tgaatctgta
    19621 taaatgccag tatccgcaag caacagcttg tgcttcgtta gctacagttc ttcccatacc
    19681 ttcttttaat ccatggttta tacatggagc ataagctatt attaaagatg gcccatcatg
    19741 tttttcagct tctataacgg ctttgataaa ttggttttta tcagcaccca tagcaacttg
    19801 agctacatat acgtttccat atgtcatagc catcattcct aaatctttct ttctagattt
    19861 tttacctgaa gcagcaaact tagccatagc agcagctgga gtagctttag aagcttgacc
    19921 tcctgtattt gaatatactt ctgtatcaaa tacaagtacg tttacatctt caccagatgc
    19981 aagaacatgg tctaatccac cgtaaccgat atcatatgcc caaccgtctc cacctagtat
    20041 ccattgagat ctcttaacta gataatcttt tctatctttt atagctttta ctaactcttt
    20101 agctttttca tctgtacaat cacaagctgg tttttctaat aattcaacaa ccttagcact
    20161 tgctacttta gaagcttctc catcatttct agaatctaac cactctgtaa atactgcttt
    20221 agcatcttca cagataccca tagaaactaa ctctgtcata gcatctacta ttttatttct
    20281 tatttgctta acagctgtgt acataccaaa tccatattca gcattatcct cgaataaaga
    20341 gtttgcccaa gatggacctt taccttcatg gttacaagta taaggaattg atggtgcaga
    20401 agctccccat atagaagaac atccagtagc attagctatc atcattctat ctccatataa
    20461 ttgtgtaact actttaatgt atgcagtttc tccacatcca gcacatgctc ctgaaaactc
    20521 cattaatggt tgtctaaatt gacttccttt aacagtgttt acaggcataa catcaccttt
    20581 tggagctacc tcatcaggat ttactgcaaa tgcccaatta tctaactctg tatcaagttg
    20641 agtatctatt ggcttcataa ctaaagcttt ttctttagct ggacatatgt cagcacagtt
    20701 tccacatcct gtacagtcaa gtggactaag ttgcattcta tattttaatc cttctaatcc
    20761 tttacctaat gcttttttag cttcaaatcc ttctggagca ttagctaatt cttcttcagt
    20821 aactaatact ggtcttatac atgaatgagg acatataaaa gaacattggt tacattgaat
    20881 acagttatct attatccatt caggaacatc aacagctata ccacgttttt cataagctgc
    20941 agtaccacaa gggaatgttc catcttctat accattgaat gtacttactg gtaagttgtt
    21001 tccttcttgt gcagtcattg gtcttagtat attttttata aattctggtt ctttaacttc
    21061 tcctttatct gcatcaacag cattttccca agaagctgga acttcaactt taactaaagc
    21121 atttattcct gcatcaacag cagcatagtt catgttaact attttttcac cttttttacc
    21181 ataagcttta actatagaat cttttaagta tttaacagca tcttcttctg gtattatttc
    21241 agctaattta aagaatgcag attgcattat catatttatt ctgtttccaa gacctatttc
    21301 ttgtcctagt ttaacagcgt taactgtata gaattttata tcatttttag ctatatattg
    21361 cttcatatga gctggtaaat gttcttcaag tcctgcttgg tcccatatag tgttaagtac
    21421 aaatgtacca ccttttttaa gaccttttaa taaatcatat tgataaacat atgattgttt
    21481 atgacaggca atataatcag cttcatctat taaataagta gctcttattg gagtatcacc
    21541 aaatcttaag tgagacatag ttataccacc agatttttta gagtcataat caaagtaagc
    21601 ttgagcatat ttttcagtat ggtcaccaat tattttgata gcttgtttat tagctccaac
    21661 agtaccgtca gaacctaatc cccagaactt acatcttata gttcctttag aagctatttt
    21721 tattggctca gatggagtta aagaagtatt agttacatca tcaactatac ctactgtaaa
    21781 tccattttta ggttgttctg aaactaagtt atcaaaaaca gtttttatat ctgaaggagt
    21841 agtatctttt gaacctaatc cgtatcttcc acctattatc ataggagcat tttcttttcc
    21901 atagaataca tcacgtacat ctaagtataa tggttcacca gttgaacctg gctctttagt
    21961 tctatcaagt acacatattc tttcaacagt agatggcata gcatctaaga aatgtttcat
    22021 tgagaatggt ctatataaat gaactttaac taaaccatat ttttctccct tagcatttaa
    22081 gtaatctata gtttcttcta ttgtttctgt tactgaaccc atagctatta atacgtattt
    22141 agcatcttct gctccatagt aatcaaataa actatgtttt ctacctgtta aattactcat
    22201 tttttccatg tatttttcaa ctatacctac tatattttga tagtatttat tagaagcttc
    22261 tctagtttgg aaatatatat ctgggttttg agcagtacca cgagcaactg gatgatttgg
    22321 agataaagca ttatctctaa atgcttgaac tgcttcaaag tttaataaac ttgcataatc
    22381 ttcattttct agtaattcaa ctttttggat ttcatgtgaa gttctgaatc catcaaagaa
    22441 atgtataaat ggtaatctac cttctattgc agctaaatgt gcaacaggtg ctatatcagc
    22501 tacttcttga actgaaccag aagctaataa tacacatcca gtttgtctag cagccataac
    22561 gtcttggtga tccccaaata ttgataacgc ttgagatgct aaagcacgag cacttacatg
    22621 gaatactcct ggtaataact ctcctgcaac tttatacatg ttaggtatca ttaataataa
    22681 accttgagat gctgtaaaag tagaagttaa agctcctcct tgtaaagaac cgtggaatgt
    22741 tcctgatgca cctgcttctg actgcatttc aacaacatta actgtttgtc caaatatatt
    22801 ttttcttcct tgtgatgccc actcatcaac tacttcagcc atagttgaag atggtgtgat
    22861 tggatatata gctgctacat ctgtaaacgc ataggcaaca tgagctgcag ctgtatttcc
    22921 atcaagtgtt ttcataaact tagccataat atgtatcctc cttgatagct acaattgtac
    22981 ttgtttataa caagtctatt tttttatatt tacatataat ttacacaaca aaactataaa
    23041 cacacatata gtcgcgcaaa tactctatct tctaatataa t
//
LOCUS       DAGSWI010000058        23053 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.058, whole genome shotgun sequence.
ACCESSION   DAGSWI010000058 DAGSWI010000000
VERSION     DAGSWI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.058"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            108..1841
                     /locus_tag="MJF80_002069"
     CDS             108..1841
                     /locus_tag="MJF80_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBZ0280730.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   2233..3056
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2298..2326
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(3336..3488)
                     /locus_tag="MJF80_002070"
     CDS             complement(3336..3488)
                     /locus_tag="MJF80_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280731.1"
                     /translation="MDNFLQGVLASLVASLIVYLNSKLFKKVKSHSGRSDFSFELKIK
                     FKKNKH"
     gene            4612..5967
                     /locus_tag="MJF80_002071"
     CDS             4612..5967
                     /locus_tag="MJF80_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBZ0280732.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVFSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(6085..6753)
                     /locus_tag="MJF80_002072"
     CDS             complement(6085..6753)
                     /locus_tag="MJF80_002072"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBZ0280733.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFEKALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(6820..7248)
                     /locus_tag="MJF80_002073"
     CDS             complement(6820..7248)
                     /locus_tag="MJF80_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBZ0280734.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            7355..7948
                     /locus_tag="MJF80_002074"
     CDS             7355..7948
                     /locus_tag="MJF80_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBZ0280735.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            8067..9002
                     /locus_tag="MJF80_002075"
     CDS             8067..9002
                     /locus_tag="MJF80_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBZ0280736.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEIMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(9273..10217)
                     /locus_tag="MJF80_002076"
     CDS             complement(9273..10217)
                     /locus_tag="MJF80_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBZ0280737.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFCKSNKLKQIEKTESI"
     gene            10535..11965
                     /locus_tag="MJF80_002077"
     CDS             10535..11965
                     /locus_tag="MJF80_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0280738.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            12165..12749
                     /locus_tag="MJF80_002078"
     CDS             12165..12749
                     /locus_tag="MJF80_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBZ0280739.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(12853..13632)
                     /locus_tag="MJF80_002079"
     CDS             complement(12853..13632)
                     /locus_tag="MJF80_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBZ0280740.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(13689..14945)
                     /locus_tag="MJF80_002080"
     CDS             complement(13689..14945)
                     /locus_tag="MJF80_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBZ0280741.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAVEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            15203..16102
                     /locus_tag="MJF80_002081"
     CDS             15203..16102
                     /locus_tag="MJF80_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBZ0280742.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEKAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            16126..17574
                     /gene="ddlR"
                     /locus_tag="MJF80_002082"
     CDS             16126..17574
                     /gene="ddlR"
                     /locus_tag="MJF80_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBZ0280743.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            17633..18439
                     /locus_tag="MJF80_002083"
     CDS             17633..18439
                     /locus_tag="MJF80_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBZ0280744.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEMLFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSKVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            18465..20384
                     /locus_tag="MJF80_002084"
     CDS             18465..20384
                     /locus_tag="MJF80_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBZ0280745.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(20626..20973)
                     /locus_tag="MJF80_002085"
     CDS             complement(20626..20973)
                     /locus_tag="MJF80_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBZ0280746.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(20960..22888)
                     /locus_tag="MJF80_002086"
     CDS             complement(20960..22888)
                     /locus_tag="MJF80_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBZ0280747.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
BASE COUNT         8631 a         3303 c         3060 g         8059 t
ORIGIN      
        1 aattaactta tttaacaaag atatcatagt ttaacaaagc ttttaattta aatatattga
       61 tgtataatat aattaaaata taatagcaaa aagaaaggag gaatatcatg agcatactcc
      121 taaaaaaagc acccaaatta gcaaaacata ttataacaag tttttatata aatcgggata
      181 ttgatgaagt attaaaatac ctttgtgaaa atgttacatg gatcggacca ggagaacagg
      241 aatttctcac ttcttttaat gaaataaaaa attattttta tgcaggtcaa gatgaaattc
      301 cttcttgtga tataaataat gatatatttg aaactgtaag tgaagatgag aatagatgta
      361 tggtattagg aagatatact gttcgaacta aagaaaatgc tcaaatgatt ttagaggtaa
      421 atcaacgttg tacatttgag ataattgagg atagaggaaa attattaata aagcacatgc
      481 acatatcaaa cccttatggt gaaatgcaac ttgatgaata ctttcccact aaaattggaa
      541 ctcaaagtta tgattatttg caaagattac taaaagaaaa aacagaagtt attgaaatga
      601 ttacaaataa tatcaatggt ggcttaaaag gtagcaatga tgatagtaca tactcatttt
      661 tctatgtcaa tgagggattg cccaaaatat taggatacac atatgatgag ttcatggaaa
      721 tgagtggtgg ttcagcagtg ggtgctgtat atccaccaga cttacctaaa gcactagaag
      781 attgtgaacg atgttttgca aaaggaccta catattcatc tgaatataga atccgaaaaa
      841 aagatggaac attaatgtgg gtattagatt ctggaatgaa atcgttaaat agtgatggca
      901 tagtaaaaat taatagtatt attactgaca taacccaact aaaaaacata gaatctgaac
      961 taaaattaga aagagagcga tatcgtattg ctttgcaaaa tattacagat atcatgtttg
     1021 agtatgatat tgaaaacgat acccttataa aatatcaacg tgtagaaatt gacaaaaaaa
     1081 ttgaattaga aaattttgaa acaaaaaatt actccaaact attagaatct ggtaaaatta
     1141 ttcaccttga tgatattgga aaactactag aagtacttca tggaaatctt cgtgaaacaa
     1201 ttgagataag acaagtaaat tcattaacta aaaatgaatg gagatggatt cgtgttcaat
     1261 gttcagttat atatgatagt gaccataacc ctatcaaaac aataggcgtt ttaaaagaca
     1321 taactgaaga taaatctaaa ttagaaagtt taattaatca ggctcaacgt gacccactaa
     1381 cacaattata taatcaaaga gtatcacaaa atcttattga agagtatcta tgtagttctg
     1441 attccaaaaa taatgatgct ctattagtta ttgatattga tgattttaaa actgtaaatg
     1501 atacttttgg gcatctagag ggaaatgaag ttttagttgc tgtatcaaaa atactattac
     1561 ataacacaca tgataaagac atagttgctc gaattggtgg agatgaattt acaatattta
     1621 taaaatcatt aacaaaggat ttaataataa aaattactaa tgatatttta aatgatgcga
     1681 gtaaaataaa agtaaaggac aatcataaaa taacattaag tattggtatt gccttcactg
     1741 atgattcaac taaattatat aaagatttat tttctaaagc tgataaagct ctttatctat
     1801 caaaagcaga tggcaaaaat tgttatagta catacgaata ataaagctat ttatacgacc
     1861 aagaatgaac ctttaaattt aatttcatag tctaacaatc aaagagttga agctaatctt
     1921 atagctagtc aactagttcc tagaaaaaat ttagacaata tatttattga tactgctcat
     1981 ttttctaaag agcaaattta tataaaaaat taaactttta tgtacataca gatactaatg
     2041 ataaacagtt atcattagta tcttctatct taaaacaaaa tgataattta tctatataaa
     2101 aaagtgcttt gttgacagag ttcacttttt tatataggta cagaatttaa gtataaatac
     2161 atgttttatt ctatacattt tagttaatgt aaaatttctt gattgtactc tctaactttt
     2221 caacattata ctatttatat accacttggt taatataaaa ctattattat agtgtcgtgt
     2281 ggagggcggt gtgacttatt tacataccac ttagttaata taaaactgtt ttctgaatct
     2341 ttttttgtat ctataatatt tttatttaca taccacttag ttaatataaa acaaatccta
     2401 attgggtagt tatccaaaag actataacat ttacatacca cttagttaat ataaaaccta
     2461 gcttttattt cctctgctgt cttatcaaca ttattattta cataccactt agttaatata
     2521 aaaccttttg ttttaggatg attatataat attacaaatt tatttacata ccacttagtt
     2581 aatataaaac ttttttaatg caaatttata aaaaaggggg tggatagaat ttacatacca
     2641 cttagttaat ataaaacttg aatttgtaaa tagttagtta taaaaattaa aagatattta
     2701 cataccactt agttaatata aaaccacaca tctgatacta aaaaatataa tgctaaagat
     2761 aatttacata ccacttagtt aatataaaac ttgatgttgt aatatgtcca gaaaaaaagt
     2821 ttaaaactaa tttacatacc acttagttaa tataaaactc attgtactcg ttaagtttat
     2881 ttgtgaaaaa atctaattta cataccactt agttaatata aaacttacag taacaaaaaa
     2941 attagatgat gttgacaatg atttacatac cacttagtta atataaaacg atttttctgc
     3001 ttcgactggt gttattgaga cttttttatt tacataccac ttagttaata taaaacccca
     3061 aaataaactg ggcattttca atgcttacac atgtgcaact ctcttaaatt tgcagtgaac
     3121 catgagtagt gcaaatgata aagcttatca catgccctca acaccttata tttcaagcat
     3181 taagccctac tttgtaacaa atatcgctca ctgcaaaact atttacattt ttattatatc
     3241 ataaatatat tatttttgaa tatctgtacc aatttgtggt ataataaaaa caagaagaac
     3301 tacaatctat ttagacatag agtgaagttc aaatactaat gtttattctt tttgaacttg
     3361 atttttagtt caaaactaaa gtcgctcctg ccagagtggc tttttacttt tttaaataat
     3421 ttactattta agtaaactat taaactggca actaaacttg ctagtacacc ttgtaaaaaa
     3481 ttatccatac atactcacct cccttctata cattgggagg ataatctttt gtatgaactc
     3541 cactctataa attgtagatt acatcttctt gctaaaaatg ttatagcata taattcttac
     3601 atattttacc tatatattac ttttctaaag attcgtctat atatttctgt atacatctac
     3661 tctatcctcg atttgttaca accccaatca aaaaagtagt tcaacttcta agttttatac
     3721 aaagcaggaa actcaattat tattagaaaa atcaaaaaat actcctatag gtataccaat
     3781 ttttttgatg ctaactcttg gattacgttt tggtgaagct gtatcactta tatggtcaga
     3841 tgtagacctt gataaaaaat ctcacatgta aatcaaacat tagtttatgt atacaataaa
     3901 ataatcttca aaggccctaa aacccctaga ttcaaaagaa gattatttgt tcctgatgaa
     3961 cttataagtt tgtttaaagg ggaaaaatta aaacaaaata agttaaaatt acagggtata
     4021 ttgaaaaatg aatttgattt gatatgctta aataaaagat tcaacccgtg agttcaacca
     4081 acttttgttg ttcattttaa aaaactatta agagataata atcctagaga tataaaagta
     4141 catgaattaa tacatgcaaa tgcgactttg atattacttt caggtactaa cataaaagct
     4201 atttataaaa tattagtaca tgcagatata aaaatagcta tgaatatata ttccatattc
     4261 tcacttatta gatgaaatga ataaagaaac atctgaaaat ataagcaaaa tactattcaa
     4321 ataatgttca gttttatgtc aattaaatgt cactcaaagt gctttttttc catttgtcag
     4381 ttaaatgtca cttaaagtac ctttttccta tttgtcagtt aaatgtcagt aatatataaa
     4441 taagatatat ttataagacc cttaaaatca ctattattta ataaaattta taattatata
     4501 ctatttgctg ttgacattgg tattatacca tagtttatca tttatattag aataaaatta
     4561 ttaattaata gtttcgtaat tttataatag atttacaggg agtgataata aatggataca
     4621 aaaatatcta taaacgaaaa catgagtcta ggtaaaagat tttttaaata cctagcacca
     4681 tctgtagttg caatgtgggt tttctcacta tatacaatgg ttgatggaat atttgtaagt
     4741 aaaggagttg gagaattagc tttagctgct gtcaatatat caatgccttt tattaatttt
     4801 atatttgcag tttctctatt attttcaact ggtgcatcta ccataattgc aatctatctg
     4861 gggaaaaaag atattaaatc tgctaacgaa gtcttttcat ttaacttagt atcaataatt
     4921 atattatcaa taattatatt agctataact ttttttaatt tagatagatt agcactattt
     4981 ttaggagcta cagaatctac aattggtatg gttaaagatt atttaggaat aataatattt
     5041 tttaacggat tctttatagt gtcttattct ttagaagtta taataaaaac tgatggcttt
     5101 ccaatattag ctactgttgg tgtaataata tctgctctta caaacatcat acttgattac
     5161 ttgtttgtaa ttgaatttgg atggggtgta aaaggtgctg gtattgcaac tggtttgtct
     5221 caagtctttt ctactatttt cttcttaata cattttttaa gaaaaaactc tactttgaat
     5281 ttttcaaaat ttagaattga ttttaagaca cttagaaaaa ttgtatttat aggttttcct
     5341 gattcaacta ctgaattaag ttgcggtata gttgttttac tttttaactt aagtcttact
     5401 aaatatattg gagaaaatgc tcttatttat tatagtgtca taaattatat aaatacatta
     5461 gtacttatga ctatgatggg tataactcaa ggtatgcaac cacttactag cttttattat
     5521 ggtgcaggaa atatagataa tgtaaaaaaa ttattaaaaa tggggataaa agccacaata
     5581 atagcatcag ttgctgtatt tgcaatttgt atggcttttt caggaccaat agtctcttta
     5641 tttatacatc cagaagaaac aatgctattc aatgaaggag ttcgtgtatt taaaatattc
     5701 tctatatctt tcttattggt aggaattaat gtaataattt ctggattctt tgtatcagta
     5761 gaaaaaccat caatttctac tgttatatct cttggtagag ggcttgtaat agttgttttt
     5821 agtctaattt caatgatact aatatttggt ggacaaggaa tatggatgac tactattgtt
     5881 tctgagttta tatgtcttat tttatcttta gtttttctaa aaaagaattt ttctacatta
     5941 gactctaatc taaataaggt tgcttagatg ataagagtag caatatatga aaatatctaa
     6001 ataaaaaata cctattatac aatcttgcaa atattaaacc tttgcaaaaa ttaggattat
     6061 ataataggta ttttatttat gtaattattt tatttctaaa tatccatagc catcttttat
     6121 taaagttcct tttacttcat attctacagg ttttttaaat attggaccat ctacgacaaa
     6181 tgtatgatac tctccatgtt ctccacatgg gtctgcacca gcatttttta tcttctccac
     6241 aagttctcta gttaattttt ttcctaaaaa ttcctctcct aagtgcttta agtttacttt
     6301 tgttacaaca ctacaaaatc cactgtctat aaattcatat actaaatcct ctctactctc
     6361 ttgccataat ggtaaatcta ctttcatacc aactgcttca catctatcag tatcccattt
     6421 tctatgactt tctatatcta tatctccata tgcacatatc gttgctccca tgtttttagc
     6481 ttttattaaa gccttctcaa agctctcttc atatccttct acaccacact cagcaactaa
     6541 taaaggtaat cctatattgc tacttacttg cttaagcata ttatattcta aattatgtgt
     6601 ccatgacttt ccatgttctt cacttatagt tgtcaagagt gccacaggct tatatccttt
     6661 tttcaacatt cgatttaatg ctaatatact atccttacca ccactaaatg acattacaaa
     6721 tttttcatca gccaatttta acacctcttt catctaactt tgttttatta acataattat
     6781 accaaatttt tgagttgcat agcaaattat attaaattgt taattcacta taaagaatgt
     6841 ccctaatatt aaaagtataa cccctatgta tttgtataaa tgcattgttt cttgtcccaa
     6901 aattacgcta tcaataaaaa atccaactaa aacttgggat acaagagtta atataagtgt
     6961 atttgatact cctaaatttg gaattgcctt tactgtaaaa tatattatta gacctccaaa
     7021 tactcctcct aataacaact tataattaaa accatttata gatactagac actttaagtc
     7081 acctgtaact attatacttg ccaaaaaaaa tattgctcct acagttaagc ttataaaggt
     7141 ggccactaat gctgttgtac tttttcctag ctctccattt ataaaagctt caagagctgt
     7201 agaaccacct gctaataatg caaatattat tgcccataat tttgtcatgg acaaacctcc
     7261 ttctatataa ttctattaat agaagtttat gatacaacct attaattaat tactaagtat
     7321 agtttaattt ttatacacta ggctttattt ggatatgaat ttctgttata attgttctat
     7381 cgattttgga ggtgatttta tgtacacaaa ttttaatatc gattttaata actttaataa
     7441 aaaagaaaat gctattaaat ttatgttaat gggagtatta ctcattatat taggattatt
     7501 atgccttaca tttaagacat taggtataaa attaatttct tggacttttg gtattgcact
     7561 tttattcttt gcatatttaa atttaaaaaa tattaatgaa ttaaaaagat atgctactaa
     7621 agaagaaatt aaaccctcta ttaatatcca atgggttcta ataattgcat gtatattact
     7681 ttttgtattt ccgcaaaaaa tacaaagcat attttcatta tttttaggat tttatttaat
     7741 ctttaatcaa ctagtagcac ttgtaaatag taagaataat ccttattcta aatttacaac
     7801 ttggaatata gttaagattc tatttggtat atgccttata ttatctccac tttttttatc
     7861 tagatttata gtatcaataa tgtctttttt cataatatta tttggactag ttctattttt
     7921 ctctggaaat acagcgagaa aatattaatt attttctcgc tgtattttta tattacttaa
     7981 aatagaattt gtttaagtta tctatttgtg gtaatatgtt ttttgtacaa gctcagcgta
     8041 caattttatt agaaataggt gattttttgg ataaatttaa atatgctttt gataataaaa
     8101 gatatcatac ttggaattac tatcttagaa atacttttgg agaaaaagtt tttaaggtat
     8161 ctataaatgc aggatttact tgcccaaaca ttgatggaag tcttggttat ggcggatgta
     8221 cttattgtag caaagaaggc tcaggtgatt ttgctggaaa ccctaaagat aatcttatca
     8281 gtcaatttta taatataaaa gaaataatgt taaaaaaatg gcctcatgca aagtatattg
     8341 gctactttca agcctataca aatacgtatg ctcctcttga agttttaaaa gaaaaatatg
     8401 agactatatt agaacttgaa gatgtcatag gtctttcaat atccacaaga cctgactgtt
     8461 tgccagatga tgttgtagaa tacttgtctg aactaaataa aagaaccaat ttatgggttg
     8521 aacttggact tcaaacaatt catgattcta catctaaaat tataaataga ggtcatgatt
     8581 ataaaacatt tgttgaaggt gttgaaaaat taaagtctaa aaatataaag gttgtcgtac
     8641 acattataaa tggtcttcct ggtgaagatt ataatatgat gatggaaaca gcaaaggcag
     8701 ttggtaaaat gggtgtagac ggtataaaaa tccatctatt gcatgttata aaagatactc
     8761 ctatggaaaa gatgttacaa aatggtatgt taactcttat ggaacaagat gaatatatca
     8821 atcttgtctg tgaccaatta gaaatacttc ctgaaactat gataatccat agactcactg
     8881 gtgatggtaa gagggatgaa ctagtaggtc ctatatggag cctaaaaaaa tgggaaattt
     8941 taaatcaaat tgatgatact ttaaaggcta gaaattctta tcagggctgt aagtttgttt
     9001 aatacaaaat aataactata gaaatggtta tataaatata gtcctttcta tagttattta
     9061 gtatttacaa aacaaaatat taatttgtac atttatacaa tatataataa aaaatagata
     9121 gtctaaactg tatctactta tattttattt gtactgtcca ctttattcta taaaatttat
     9181 aaatagttat tttttaatac caaatataat ttggtataat ttttgttgga ctctaaaatg
     9241 tataccaata aatccttttt aagtatataa ttctaaatac tttcagtttt ttcaatctgc
     9301 tttaacttgt tggacttaca aaatattctt atttcatcat aagaaagatt aagtgctact
     9361 actgcagata ttaacatcaa catcactcca aataacttcc ctaaggaaaa actttctcct
     9421 aatatagtgc aaccaattat tactgaacca actaattcta ctgatgccac aacacctgca
     9481 actgacaact ctacaccttt agatatacct gctgcataaa aaatatatgc aagtgcagct
     9541 ggaacaatgc ctaacattag catacaagat aaaacatcta aatccttagc ataatttaag
     9601 atttcccaag gtcttgaaga aggaatcata aatatagctc caaataagaa actgtatact
     9661 aatattgttg aacttgaaaa ttcctttaaa gcatttttac taatagttgg cataagtgca
     9721 tatgctattg ctgataatac tccaagtaaa agcccaaaag cgtttaaacc ttgaaaatcc
     9781 aaacgtccac cagtcactgc cataattgca ccaataaaac aaagaataag tgaaaataat
     9841 ttgcttctag taatattttc tttataacat aattttgaaa atattgctaa aaacagtggt
     9901 gatgtatata gtaaaactgc tgcaattgat acacccgcaa tgtcaattgc tttaaaataa
     9961 cataaattaa acattgcctg acatataatt cctattatta ctgaatatat aattcctttt
    10021 ttgtttatct ttaagatttg tggtgtcctt atagatgaat atacgcctaa aactataaat
    10081 ccaaaaaaca atcttgtaaa tgctacttgt tctgatgtca gtccatttcc cattaaaaac
    10141 tttccaaata atccaagtgt agcccataat aaccctgcta ttgctataaa tacataccct
    10201 ttactttttt ctcccatttt aaattccctt ctatttttat ttagtcaatt tacactcttc
    10261 tttttaagca aaaactatgc catcttaaat caatatatat cgactctaaa accctagtct
    10321 ttccaataat actaataaat tttcaatttt tttatattta tttttcgctt actttaagtt
    10381 gatttagact tattttaatt gtaaaatgag ttatatataa cttatttaaa gttaaaatag
    10441 actgaaactt atatataaat atcaaatttt aagtatatta atcaattaaa cctattttaa
    10501 tatatcatta aaaatttagg aggaaaatat aaaaatgaat ttagatttgg aattttatca
    10561 aaaaatatta gaagcttccc atgatgaaat atgtgtttct gatgataaag gaataataat
    10621 atattgtaat aaagcatttg aagaaaatta tggtctaaaa aaagaagata ttttgggaaa
    10681 aaatgtttct tttttagaag actctggata ttcaacaaaa agccccatac caattgtatt
    10741 aaaaacaaag actaaatttt cactggaaca agatactcaa acagggaaaa agcttatcat
    10801 aactgctacc cctatatttg atgaaaatgg acacttagaa tttactgttg aaaattgtag
    10861 agatataact gaacttaata atataaaaaa taaacttgaa gacacaaaaa agcaagtaaa
    10921 aaaatataaa tctgaagtag aaacactata cagaactgca cttagaattg aagatactgt
    10981 aattatggac ggaatagtta tgagaccaat cataaacaca gtaaatcatg tttctaaaac
    11041 ggatgtaagt gtacttttat tgggtgaatc tggaacagga aaaagctcac ttgctagata
    11101 tatacaccac aacagcaata gatctaatgg cccatttata acaataaatt gtgcaactat
    11161 atctccacaa cttcttgaat ctgaactttt tggatatact tcaggtgctt ttacaggtgc
    11221 aagtactaaa ggtaaagttg ggctcgtaga attagctaat ggaggaactc tttttttaga
    11281 tgaaatagga gatataccac aaaatctaca ggcaaaattt ttgcaattaa tacaggatag
    11341 aacttttact ccagttggga gcttaaaaaa taaaaaggtc gatataagaa ttatatctgc
    11401 tacaaatgtt gatttagttt caaaagtaaa agaaaaaaag tttagagaag atttatacta
    11461 tagattaaat gttatagaaa taaaattgcc tcctcttaga gaaagaaggg ataatctagt
    11521 ggaaattata aaatattatt ttaataggta ctcaagtgac ttcaatctaa ataaaactat
    11581 ttctaaagaa gctatggatg ctattgcaaa ttatagattc ccaggcaata taagagaatt
    11641 acaaaatata attcaaaaaa tcttgcttac ttgtacagat aatcatataa caatagatga
    11701 cttaccaaac attcttacaa aaaatataca tattaccaat aatggaaata aaacacatat
    11761 atcccaaatt aataaggtta ttatatctga ctcaaaatct actaattata aaaataaaaa
    11821 ttttgacact ttaattaaag agtatgaaaa aaatataatt ttggatgctt atgaaaaatt
    11881 tggtagttct tataaagtag ccaaacacct tgaaatctca cagtctaaag ccaataggtt
    11941 aattagaaag tatactaata cttaatttta aatttgataa ataaaaactt aacactattt
    12001 acatattgta aatttggcca tttactgtta ttatgaatat ggttgaaatt atttaaataa
    12061 atactgttga cttagtttca tatatttacc attaaacttt tatcataaat atcatatcat
    12121 aataatatat ttaataagtc taataaacta tacgaggtgt aaatatgtta atgaataccc
    12181 attttattat ggcaaaatca atacttgata atatagatga aaataaaaca ttctttataa
    12241 gcgaaaaaaa ttttatatat ggcaatataa agcctgatgc tttttctaaa tataaattaa
    12301 aaaaacatta tttagatgag tcatttaata tgattgtttc taaaataaac tatctatgca
    12361 gtcttaatat agatagtctt tctaaatact tttcagtaag tagacttagt caggaactag
    12421 gagtcatatg tcatttttta tgtgattttt tctgtgtagc tcacagtgaa cgttgggagt
    12481 ttaagcatag cttaaataaa catgttactt atgaaagaga acttggagca tttgcaaaag
    12541 atgttgattt atctaaaatt aaatgtggta ctataaatag tagttttgag aagtttttta
    12601 ctaaacttta tagtgattac aaaaagaaaa ctgattatat gaatgattta aaattttcag
    12661 cttatacttg taatagtgtc ataaattata tacttgactc tattttatct aatacaataa
    12721 aatcatatca tatagcaaat tgcggttaac ttaagaattt ttaaacttat ataaacttat
    12781 aattgaatat aattaaaatg actgctctat agaatataat tttatacttc tctaggacag
    12841 tcatttttta tactaactat ttatctccat tataacatct atggcacttt ttacactatc
    12901 tactacataa tttgcacttt gctttacatc ttcccttgcc ttagacattg caaaactata
    12961 tttaaatcct tctaacatgc aaatatcatt tggagaatcc ccaatagtag caacttcatc
    13021 tggacttaca tttaacgttt ttattacatg ctctatactt cctctttttg acacactttt
    13081 aggaactata tttaaagtat atgttccaga aaaatatatt tctaagactt cactaaactc
    13141 actttttaga taaagttcta ttctggataa ctcttccaaa ttttcagaac atacgcctat
    13201 atttcctata agattatcaa aactacttct tttttcaaaa tctgggtcaa ctataaagtc
    13261 actagaataa tgtctaaaag cagtggtatc actacccaca attacatcac ccttatattt
    13321 aaaataaagt tgtgcatcac ttgactcaaa tctatcaatg acttttttta ctaaattatc
    13381 gtctatcgtt cttttaaaaa ttacattata atctttatca tatataaatg aaccattttc
    13441 acatacataa tatccattca tatctaaact atctttgata atattatgtg cttgtttaaa
    13501 aattctacct gtagcaacat taaactgtac tccctttttt tctatttctt caattgctac
    13561 tatatcttct tttgttatgg ttccattttc atatagagtt ccatctaaat cacaaaatat
    13621 atgttttatc ataaattaaa agcctccaaa acaattttat attttactaa tactttaatc
    13681 aaaataactt aaagcttttc taattgtatt ctcaattcat taagtaaatc tctagtagct
    13741 cttaatctta tctttcttct atccccatta aatatatttt tctttactat tgtttttcca
    13801 tttatatata ttgcagtata aaccagtccc acaggcttct ctttggttcc tccaccaggt
    13861 ccagctactc cagttgtaga tatacctata ttagctttaa gtcctttagc aacgccctca
    13921 gccatttcta cagctgtttg ctcacttact gctccaaact cttccaaagt ttctcttttt
    13981 actcctaatc ttttcatttt agaatcatta gaatatgtta cacaaccttc aagaaataca
    14041 gaggatatac ctggatagtt tataagagat gaagaaacca aacctccagt acaagattca
    14101 gccactgcta ttgttagatt tttttctaca agtagcttag aaacagcatc ttctaaagca
    14161 gtctcccctt cagatatgga aatatcgcct tcagtatata catatttacc aactctactt
    14221 tttatttttt cttctactgg ttttattaat ttaaaagctt ctctttcatt ttcagccttt
    14281 gcagttattc ttattgtaac ttctaattct tttgcatata aagcaactgt tggattagtc
    14341 tgctcattaa ttatatcgag aatttctaat tctagatttg actcacctat tccatataat
    14401 cttaaagtct tagatactag catttcattt gtgaattgtt gtaagtaagg tttgacactc
    14461 tcattaaaca tagctttcat ttctcttgga ggtccaggca atactattat aaatttacca
    14521 tcttttttta aaattgcccc tggagctgta ccattattat tttttagtac tatagcatct
    14581 tttggaaagt acacttgttt catattattt tcggccattt ttttccctgt tttaacaaag
    14641 aaagattcca aaacctctag tgaaggttta tgtagttcta atttctgacc aaagtattct
    14701 gcagcagttt cttttgtcat atcatcacca gttggcccaa gaccacctgt agtaattaca
    14761 aaatcacttc tctttaagct ttcatcaaaa cattctaaaa gtctttgttt gttatctcca
    14821 acagtactct gatggtatac ttctattccc aatgatgcaa gttctttagc taaaaattga
    14881 gaatttgtat ttactatatc tcccaataat atttctgttc ctacacttat tatttcagct
    14941 ttcaaaattt atcactcctt gatttatagc cattaagact agtcttaagt tcataacaat
    15001 aattttagca aaaataataa cataaataaa gttaaaaaat catttaaaca tatctttaaa
    15061 ctagattatt attctaaaac cacacataat ataaacttcg tatatagtgg tactttcatc
    15121 ctcaactgtt aattcatata attaagtatg taaataaaaa gtttacttta aaataataaa
    15181 aattaaaata ggagtgtata atatgaaaat agcagttata atgggtggaa tatcatcaga
    15241 gagagaagtt tctttaaatt caggaaaaga gatatataat aaccttgata aaaataaata
    15301 tgaagttgta aaagtaataa tagatgataa aaaagatata tttacaaaga taccagaaga
    15361 tatagatttt gcaatacttg ctttacatgg aaaatttggt gaagatggat gtatacaatc
    15421 aatacttgag actatggata taccatactc tggatgtgga cctctttgca gtgggatgtg
    15481 tatggataaa aatataacta aaaaaatgct tagagattca aacctaccta ctgctccatg
    15541 ggttttagta aaatcagttg atgaaataga ctatgatgaa atagataata taggttatcc
    15601 agtatttata aaaccaaact caggaggttc aagtgtagcc acattcttta ttcattctaa
    15661 ggatgaagtt gaaaaagcag ttagaaaagg tctggaagtt gatgaatttg taatgattga
    15721 aaaatatata ccaggtggag aatatacttc attcatatta aatggagaag tgttccctac
    15781 aatatctata aaatctgatt caggattctt tgattatgaa gctaaatact cagttgaaaa
    15841 aggtgcaaag gaagaagttg tttacttaga tgaagaactt caaaaaagag taaacgaaat
    15901 ttctgagact tgttggaaaa tttttaattg caaagcatat gtaagagttg atatgattat
    15961 aagtgaaggt attccatatg ttcttgaact aaatactctt cctggaatga ctcaaactag
    16021 tttaattcct agaagtgctg ctgctagagg tattaagtac agtgaactat tagataaact
    16081 tatagaatac tctttaaatt agatgaaata atttgaggtg gtaatttgat attttcaaat
    16141 ctaaaattaa atgataatga accaatttat atacaactta aaaattatat aagtgatatg
    16201 atatcaaaag gactaattcc agacaatagt aagctccctt ctacaaggga gcttagtcag
    16261 cttttacaag ttagccgaaa ttctgtagtt ttaacttatg aagaattaaa atcagaagga
    16321 ttaatctact ccatttcagg aaaaggtaca tttgttaaat caaagaataa atcttcaaat
    16381 actacatggt cattaaactg ggattgttta gagaatacat attctaaaaa agccaatgaa
    16441 ttggacatta taaaaagtga aattccttgg agctctgacc tgatttcctt taaaagtatt
    16501 tctccagatg gagatttatt cgacatggaa gaattaaaaa aatccttttt aaatagaatt
    16561 tcactagaag gacataaatt actcaactat ggatatgcac aaggttataa gccccttatt
    16621 gactatttac tagaatatat gacaaacaag ggtgctgata taagtaataa agatatacta
    16681 ataacaaatg gattcacaga aggctttgat attattatgt catcttttac ccaagaaaaa
    16741 gactacataa tatgtgaaaa tcctactcat aatactgcta taaaaattat gaaatctcat
    16801 aacctcaata tcttaggtgt agatataaat gaagatggtt tggacttcaa tatgctagaa
    16861 gacaatttag ttaagtataa aaataaaata aagtttgcat acattacacc ctcttatcac
    16921 aatcctacag gaattgtaat gactcctgaa aataggtaca aattctataa tcttatgaaa
    16981 aaatacaata tagcaattat agaagatgga tttaatgaag aattactcta caatagttct
    17041 catatatttc ctatctgttc cctagataat atgaataatg gggtcgtata tattggtagt
    17101 ttttcaaaaa ttttatttcc tggaatgaga attggatgga tatttgcaga taaaaaagta
    17161 atcaataaac tagaaagtgt aaaaaggtgc agaaacatac atgtatcctt tttagaccaa
    17221 ggaattttat ttgattattt aagtaatgga ggttttgaaa aatacattaa aaaaatacgt
    17281 aaattctatg gagacaaatt taactttgca tataagtgta taaaaaaata tataaaaaca
    17341 gaatatatct tgggtgatgg tggtttgcat atatttataa aactaaaaaa tatagatact
    17401 agagttttat tagaaaaatg ttatgaaaaa aatgttatat ttatgcctgg agatttattt
    17461 tttacagata actctggttt tgatacttta agactcggat tttctaggtt atcttttgaa
    17521 gacattgaaa ttggaataaa aataattgga gaaactataa atgaaatgtt ctaggttaac
    17581 taaatattgt tagcctagtt tatttttatg aggtataata tatatagttt atatgaatct
    17641 aatactgggg gataatatga gtaaaaagtc aaaacgaaga aaaattaata aattaaaatt
    17701 aacgattgta ataactgtat tatttcttgc tttattatct atatatgaga tgctgttctc
    17761 taatcccaac aaaaaaggta atataaagag tcaagaaact atgcaaaata aaaataatac
    17821 aacagatgat tctaaaaata atcaaagctc taccaaaaag caggaagata ataatatcaa
    17881 acctactttt tcatattctt caataccaga taatataaaa aataaaatga ttggtaaatc
    17941 tatgcctact gatgaaccta taagctttga tagtctatct tatttaaaac ttacatatta
    18001 tggatttgat gaaaaaactc atcaaggaga aatgatagta aacagtgaac tagctccaga
    18061 agtagtggat atttttaagg aattatacga aaaaaaatat ccaattgaaa aaattaagtt
    18121 aatagatgac tatgatgctg tagatgaaaa atctatgtct gataataata cctcatcatt
    18181 ttgttataga actatagctg gtacaaatgt agtttcaaat catggtaaag gacgtgctat
    18241 agatataaac ccattacaga atcctcaagt ttctggaaat gatgttacac ctaaagtaag
    18301 taaagtttat gctgataggt ctagcacaaa atttggaatg ataaaaaaag gtgatgattg
    18361 ttataatgct tttgtaagca gaggttggag ttggggtggc tattggaaaa acccagatta
    18421 tcaacatttt gaaaaataat agattagaaa taaaggtgaa ttatatgaat ttaatgacat
    18481 tggaaaatat aagtaaaagc tattctgaaa aaaaattact agaaaatata tctcttggaa
    18541 taaatgaagg ggaaaaaata ggacttattg gtgtaaatgg tactggtaaa tctaccttac
    18601 tcaaaattat tgcaggagtt gaagaaagtg agactggtaa tataacaaaa actaatggta
    18661 ttaggataga atatctacct caaaatccaa cttacaatga aaattcaact gtacttgaac
    18721 aagtatttaa tggaacttct gaagaattaa agctacttgg tgaatatcaa gaaatcctag
    18781 aaaaacttaa ttcaaacttt acagaggaat taaataaaaa attattatca gtacacgaaa
    18841 aaatagatgc cttaaatctt tgggatttag aaagtgaagc taagtcagta cttacaaagc
    18901 ttggtataaa tgactttaat caaaaaatag cagaactttc tggtgggcaa agaaagagag
    18961 tctctctggc atcagccctt attacacctt gtgagttttt gatactagat gagcctacaa
    19021 accatctaga caatgatact atagattggc ttgaggaata tcttaattcc agaaaaggtt
    19081 cattgcttat gataactcat gatagatact ttttagaccg tgttacaaat agaattattg
    19141 aactggacaa gggtagatta ttcagttatg atgggaatta ttcaatattt ttggaaaaga
    19201 aaatggaaag actttcgcta gaagcaagta ttgaagataa aagacaaaat ttaataagaa
    19261 cagagcttgc gtgggtaaga cgaggagctc aggctcgtag cactaaacaa aaagctcgtt
    19321 tacaaagatt tgatgaactt ataaatagag attcttttaa acctgatgaa aatctcgata
    19381 tatctgttgc atcaagtaga cttggaaata aaataataga aatacacaat atatctaaat
    19441 catttgaaga aaatactgtt atagataatt tagaatacac tttaactcgt acagatagaa
    19501 ttggtataat aggcaaaaat ggtatgggta aatctaccct tataaatata cttaatggag
    19561 aaatagagcc tgatagtgga tatatttcaa ttggagaaac ggttaaaata ggttgttttt
    19621 ctcaagacga ctctcatatg aatattgata tgagggctat agactatgta aaagaagcta
    19681 gtgactatat agaaacttca gatggtacta ggattacagc ttctcaaatg tgtgaaagat
    19741 tcctttttaa tggtactatg caatatactt tgataggtaa attatctggt ggtgaaagaa
    19801 gaagactaca tcttcttaaa atccttatga cagctcctaa tgtgcttctt ttagatgaac
    19861 ctacaaatga tttagatatt gaaacgctta aaatattaga agaataccta gacgaattta
    19921 aaggtgtagt agttacagta tcacatgata gatatttctt agatagaatt tgtaataaaa
    19981 tatttgcata tgaaggtaat ggaaaaattc atatctatac aggtaattac agtgattatc
    20041 ttatttatag agagattcaa ggaattgaat ttgaagaatc taaaaaggaa tcagttaaaa
    20101 aagatgactc tactacccta aaaaaagaaa aacctaaaaa tgataaaaag cttaagttct
    20161 catataatga acaaagggag tttgaaaata ttgactctga tatagaaaaa ctcgaatcta
    20221 aaattgcaac acttgatgaa agtactgcaa aattttcaac tgattttact aaacttcaag
    20281 aaattttaga tgagaaatct aaactagaaa aagaacttga atacaaatat gaaagatggg
    20341 aatatttaaa taatctagct gatgaaatag ctaataataa gtgatattaa aataatctta
    20401 agtttaataa gttttgcttt aaatctgtac tttataaaca ttttaaagga agtgtctcaa
    20461 aatgaacttt ttagttttat gagacgctct tttttatgag aaatcaaata tgttttcatc
    20521 atttcaacaa tacaaaagag gcttatctct attttgagac agcctctttc ataattacct
    20581 tctaatttat atgctctaat atatctataa taattcttat tatatctata tatccagtct
    20641 ttcaatatag tgacaattaa cttcaatatg atttaaaaat actaacaacc tttcataaag
    20701 tggatgagat tcttctccat atttagcttc taggttcttg cctatttctc taacagtcct
    20761 ttgaccatca atttgtaaaa aaacaaaact ggaatattca tctagttcta tttttttgta
    20821 tttaggtatt ctagctttta actttctaaa aaacttttga attttatggt cctgtttttc
    20881 aagtatagta acaacactat tttcagctac ttcatattca agtccatcag taattttaaa
    20941 tacaatatct aaaacatcat tactcttgct cattattttt tactttagat aaaacaatag
    21001 gtaatgctgt aagcacaact aaaactacta ataaagcaat tgccattgaa tttgttgcag
    21061 caaatccact tggtaccttt ggagttacaa ctccagttac ttgtaatatt atacctatta
    21121 aaccaatgat agaaccacca gcaacaagac cagaagataa acttatacca ttagaaactt
    21181 taacttcttt ttctttttca gttttagata ctaattcaac aaataaacga attaatgctc
    21241 caactaatat tatagaagtt gttgcaattg gtaaatagaa tcctatagct atagtcatta
    21301 ttggtaaatt taatgcatat agaactattg ccataaatac tccaacaata atcataaccc
    21361 aaggtaaact acctgacata ataccagaag ttaatgttga cattaagttt gcttgtggta
    21421 atgcaaattg tacattatca ccagttgctc taagttggtc agaaagtaat aaaattactc
    21481 ctacaacaac tataacacca actatacttg caattgtaaa gtaacgttgc atttcgtttt
    21541 tgctaccacc aataatataa gttacttttt gagattgagt ataacctcca gctatagcaa
    21601 tagcaacaac tataaatgaa ccaaataaaa gtaatgattt attagcttct aaatcagtcc
    21661 atcccattat aacaaacact aatgttacaa taactagaga agctatagtc ataccagaaa
    21721 ctggaagatt tgatgttccg attgttcctg ttaaacgacc agctactatg acaaataata
    21781 aagataataa aagagatatg atagctccaa taattgccat aacaatgtta ccagaaatta
    21841 agaatgctgc taaaaatcca ataactactc cacctaataa aagtatcatt tgtatagatg
    21901 aaccttcttc tccctctcca gcacttgatt tagctttaag tgtttctttt atagaagcga
    21961 ttatagttgg tataagtttt attgcaccaa taatacctcc acaaagcatc ataccagcac
    22021 ctatatattt tacataacta ctagatattg ctccaacatc catttggtta agaggcattg
    22081 caggattatt ccataccata gctccatctt tagccatatc tgtaaagtat cctattaaag
    22141 gtgctatacc aaagtttgat aaaatacttc ctgcaaacat tgttaaagaa acttcaagcc
    22201 ctactataaa tccaattcct aaaagtagag gatttacttc aatatcaaat ttccatttgt
    22261 agaatttgct tcctacaaga ctcattacat tgtttgctac atttaagaat gaaccagtta
    22321 atacagtaat aaatccactt attacaaatc caattcccat gtacttaatt gaatctccac
    22381 cagcctctga agcaacaagt gtctcagata tagccattga ttctgggtac ataagtttac
    22441 catgttcttc aactattaga tagttgtgaa ctatagcagc aattcctata ccaaataaaa
    22501 ctccaccaac tccaacagct aagccttcaa agaatgtaat ttggcttcca attaatataa
    22561 ctgctggtaa tacgaaaata aatccacttg caacagattc tccaccactt gacatacctt
    22621 ggattaagtt tttaccaaga ataccctttg aacgagcaaa tgctccaaca aaagcagaac
    22681 ctattatagc tcctggaata cctgcggcaa ctgtaagacc ggctttcatt ccagagtaag
    22741 ttgtagatgc ggcaaaaatg gcagctaaaa taataccgat aatcagtaca gctacatttc
    22801 caccggttct ggacttatct gtgatatatg gaacgtaatc ttttccacta acctcaccat
    22861 aagcaccctt aggtaatttt ttgttcataa aattttcacc ccttttcaat ttgtatatat
    22921 tataacatat atacatataa aacttaactt tttattattt tacaaatagc gagtcaaaac
    22981 tactaaaatt taaccctata ttattaattt ctacttttag atatttttat gctaattttc
    23041 tttttatttt tca
//
LOCUS       DAGSWI010000059        23024 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.059, whole genome shotgun sequence.
ACCESSION   DAGSWI010000059 DAGSWI010000000
VERSION     DAGSWI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.059"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..354)
                     /locus_tag="MJF80_002087"
     CDS             complement(<1..354)
                     /locus_tag="MJF80_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0280748.1"
                     /translation="MKKIPKSELLVMNFIWDKGIDKIPAREVANYMEISHGWTKGTTG
                     KVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISSLDC
                     GNEISDDDIKELEQWL"
     gene            complement(882..1304)
                     /locus_tag="MJF80_002088"
     CDS             complement(882..1304)
                     /locus_tag="MJF80_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBZ0280749.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALIRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            1782..4685
                     /locus_tag="MJF80_002089"
     CDS             1782..4685
                     /locus_tag="MJF80_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0280750.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            4718..5164
                     /locus_tag="MJF80_002090"
     CDS             4718..5164
                     /locus_tag="MJF80_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0280751.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            5287..5589
                     /locus_tag="MJF80_002091"
     CDS             5287..5589
                     /locus_tag="MJF80_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0280752.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            5770..7176
                     /locus_tag="MJF80_002092"
     CDS             5770..7176
                     /locus_tag="MJF80_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBZ0280753.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            7520..8578
                     /locus_tag="MJF80_002093"
     CDS             7520..8578
                     /locus_tag="MJF80_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBZ0280754.1"
                     /translation="MGDKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCRKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            8677..9363
                     /gene="araD"
                     /locus_tag="MJF80_002094"
     CDS             8677..9363
                     /gene="araD"
                     /locus_tag="MJF80_002094"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBZ0280755.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            9381..10028
                     /locus_tag="MJF80_002095"
     CDS             9381..10028
                     /locus_tag="MJF80_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBZ0280756.1"
                     /translation="MLDSLKGKIVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GIGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPILPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(10201..11568)
                     /locus_tag="MJF80_002096"
     CDS             complement(10201..11568)
                     /locus_tag="MJF80_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBZ0280757.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(11758..11846)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            12274..14394
                     /locus_tag="MJF80_002097"
     CDS             12274..14394
                     /locus_tag="MJF80_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBZ0280758.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNILEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            14498..15751
                     /locus_tag="MJF80_002098"
     CDS             14498..15751
                     /locus_tag="MJF80_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBZ0280759.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDSTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(15905..16966)
                     /gene="ytvI"
                     /locus_tag="MJF80_002099"
     CDS             complement(15905..16966)
                     /gene="ytvI"
                     /locus_tag="MJF80_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBZ0280760.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            17240..17980
                     /locus_tag="MJF80_002100"
     CDS             17240..17980
                     /locus_tag="MJF80_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBZ0280761.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            17992..19770
                     /locus_tag="MJF80_002101"
     CDS             17992..19770
                     /locus_tag="MJF80_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBZ0280762.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            19937..20854
                     /gene="pfkB"
                     /locus_tag="MJF80_002102"
     CDS             19937..20854
                     /gene="pfkB"
                     /locus_tag="MJF80_002102"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBZ0280763.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            20891..22810
                     /locus_tag="MJF80_002103"
     CDS             20891..22810
                     /locus_tag="MJF80_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0280764.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIFLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT         9177 a         2498 c         3908 g         7441 t
ORIGIN      
        1 tagccattgt tcaagttctt ttatgtcatc atctgatatt tcatttccac aatctaatga
       61 tgatataaag ctatgaatag aatttttatg tataagctct aaaaaatcct ttgtttcaaa
      121 ttttagatat tcctcatgtt taactttaat agtgtataaa gtatttctac catctttttc
      181 aaattctaaa aaacctttat cacttaatct agaaagtact ttaccagtag taccttttgt
      241 ccatccatga gaaatttcca tataattagc tacttctctg gctggtattt tatctatgcc
      301 tttatcccat atgaaattca taactaacaa ctcggatttt ggtatttttt tcaatataat
      361 cactcacctt taacttgatt atattagaca aatgtctaat agtcaatata atatatcata
      421 atgtataaat aatctttata ttattttatg cttgagaact aagtagtata gatttataga
      481 taaaaaagat gttgagagtt ttatggaaga ggggaatata ttttctatta gagataagaa
      541 tatgacatat attttttatt gatttgtaca aaaatactat atagtgaata aattaatatc
      601 ttttctaaat tagaagagga taatcttatt agataaagat tatcctcttt attgaaagtt
      661 taagggatat tagagattat taatttttct ctcttttttt aactaaaagc aataatacat
      721 aaaattatta ttgctaatga tactcttaat gtggtatata gtcatagtgt cacatgttta
      781 tcaatagttt tttcagtaat tattgtaacg aatagtagga ctcataaaat acttagaaat
      841 atgtttttag ataaatttta aatatataat ctaacttata tctataaatt aacactatta
      901 ttctccaatt ccatttctag tttttttata aatttattaa attcttctgg ttgccttttt
      961 ccaaacaata ctcttataag agctgaattg atttttccaa atatattcct cttgctctca
     1021 ctttcaataa aaataagttt tgtattttct aagtcaatat gtactaattc atatctagta
     1081 atagtacttt ctcttttatt gcttgttttt atctcatata ctctattttt ttcgaaatta
     1141 gttatttcaa ccttaaatct tttattattt ttttgctgta caaatgttcc tactgctttt
     1201 ttctcattaa aattaggaaa atttagtctt gcagattttg taaatatatt aaatatgtct
     1261 tttgcagaat attttaataa aacttcagtt ttaaatgtat tcatatcgtt ttcccttctg
     1321 tatataaatt taatatttat aagttaattt ttaagttaaa atagtataat atgccatatt
     1381 aaatcatgtt aaaactattt atatagctaa aatcttacct atataagtat gtatactatc
     1441 atataaaaat aaaatcttaa aatagaaagg gttaaaaaca cagtttatta taatttttat
     1501 tgttgagtag atacgtacta ttatatatga ttgagcaagt ttttcaatca taaatttata
     1561 aagtattttg cattaaaatt taaacaaaga attaaattat ttaagattat gaataactaa
     1621 tacatttagt aaattactaa atatacaaaa tgtaaatact tatcaaaatg tataaatact
     1681 tatgacatct agttacttac agggtttaag tagaaaaaaa tatcaaagta agtatggcat
     1741 gatagttgct tatatattta tcaagaaagg agttattaag catggagttg agagataaac
     1801 ttgtaaacat cataaataca gaaaataaaa aagatccatt aacagatgtt caaatagcaa
     1861 agtttttaag tacaactaga gagaacataa ctaatttgag aaaagaactt aatataagta
     1921 attcaaggca aagaagatat ccttatttaa aatcagcaat ttcggcaata ttacaaaaga
     1981 ataagaatat ttctatatca gaaattacaa gggaattgat gacagaaggc tttaatatat
     2041 ctagaagagt tgtggaggaa cttttaccta aagaaagttt agaaatggat gtagtagagg
     2101 aatctgaaga agaatcgaat gacccatttg aaactctgat aggtaataaa gggagtctta
     2161 gaaatgctgt agagcaggct aaatctgcta tattgtatcc accaaaaggg ttaccaacgt
     2221 tgattattgg ggaaagtgga gttggtaaga gtttattttc tagacatatg tatgagtttg
     2281 caaagcaaaa aaagatagta aaagaaagct ccaattttgt tgtatttaac tgtgctgatt
     2341 atagtgacaa tccacaatta ttattatctt tgttatttgg atataaaaaa ggtgcattta
     2401 caggtgctga atatgataca ccaggtcttg tagaagaagc tgatgaagga gttttatttt
     2461 tggatgaaat acatagattg cctccaaaag gccaagaaat actattttct attttagata
     2521 ggggaaagtt taggagatta ggtgaaacta atgctgaaag aaaagtaagt attatgttga
     2581 ttggggcaac tactgaaaat gtagagacta atttattatt aacattcaga agaaggatac
     2641 caatgctaat aactttgcca ccattacatg atagattact aaaagagaaa atagacctta
     2701 tatacaatat ctttcaacaa gagtgtaata gaataaatgc aaaaatattt gtagataaaa
     2761 atgtgattga aatacttgca ctaaaaaagt ttagtggaaa tataggacaa ttacaaaata
     2821 tgattcaggt tctttgtgca agagctttta tgaagtttat aaattccaaa gatgaagata
     2881 gtgaatcgat agtagtagta gatataaatg aagttttaaa gcttaaagat tcttttaaag
     2941 atgctgcctt tcaagagatt gaatatacag agataagaaa atatttgaaa aatgctatat
     3001 ttataccatt taatttagaa gaatcatcag taaaaggaag tcttttaagt gatgaatata
     3061 tattaccaga ggacatatac aaaacaattg aaaagaaata ttatgaccta aaatccctag
     3121 acattgaaga tatagatata gagaatattt tatggacttt tattttaaat agatttgctg
     3181 acttaaagtt taatctcgat agtaatgaag aaattttttc aatgagtgat ttaaatagtt
     3241 ttgtaagtga tagtttagtt aaactggtaa aagaacttat gaatgatata gttgaaaaaa
     3301 atatgaattt agaagtaaat aaaaatatgt tcaaatatct agcaatacac ttagaagaag
     3361 ctataaaaag aattaagcta aatcaaaaga taataaatgt aaatttagaa aaaataaaga
     3421 ttgacttttc aaaagaatat gaaattagta aaagttttgc aataaaaatt gaggaatcaa
     3481 ttggtattaa gattccagat gatgaaattg gatttataac actctacata aagtctgctc
     3541 taaaaaatga agtgaaaaaa gataaagtag ggttaataat aatttctcat ggacgtatag
     3601 ctacagagac tgtaaatgta gttaaagaac ttttaggtgt aaagtttcct gtggctatag
     3661 atatgccttt agatgaaaaa ccaataaata tatacaataa ggcagtagaa ctgtcaaaaa
     3721 taatagacca agggaaaggc atattgtttt tggtggatat tggttcactt acaaacatag
     3781 gtcaaatagt aaataagaga accaatataa gcactaaaac tattgataga gtagacctcc
     3841 taatggcact agaagcaact agaaaagttt ctatgggaga ggaagaactt gatgaaatat
     3901 ttttttcttt gctaaaagat aggatgggat ataactatga attagaaaaa cacatagata
     3961 aatcaaatgc aattataaca ttatgtctta ctggagaagg aactgctaag tatataagta
     4021 aaacgttgga agaaaagtat gataacacta aatgttatca aatgagtgca ttggatgaaa
     4081 atttattttg taaaattgaa aatctaaaag agacaaacaa tatattagct attgttggga
     4141 caataaaccc taagatacca ggtataaatt ttatacccta taataaagaa cttttcaaaa
     4201 atttggatat ctatctatca aaatcaaaac aacaagatga cagaccaatt aaatcgtatg
     4261 aaagaatgct tgatgaggat ttagtgattt ttgaaccaga tatatatttt aaaaaggatt
     4321 tattagaata tgtatgttca attcttataa ataaagaata tgtagaaaaa gattacttag
     4381 attctgtttt acatagagaa gaaatgcttc caacatattc taaaggagct ataggtgtgc
     4441 ctcatggaga ttcttctact gtaaatagta caaggtttgt ttttgttaaa ctaaagaatc
     4501 ctattgattg gggtgtaggt aatgtaaatt tcatatttat gccagttttt caggctaatg
     4561 ataaagagat tgttaaaaat gttcttgaga tactaaaaga tgcagaattt atgaacaatg
     4621 ccaataagtg ttttgataaa gatgctttta gaaggattat atttgataaa tttaaacatc
     4681 tgtagccata gcaaaaaaat tgagaggagt tagagtaatg aatgtagatt taatatcatt
     4741 gggtttagaa tctaatgatt atgaaagtgt tattgaagaa ttaggaagta taatgtgtaa
     4801 aaaagaatat gtaaaagaaa catacataaa tgctgtcttg gaaagagaaa ggacattacc
     4861 aacaggtcta gatattggag aaatgtgtgt tgctattcca catacagatt caaagcatgt
     4921 aaatgagtct aatgtagctg tagggatatt gaaaaatcct gtaaagttta attcaatgat
     4981 agacccaaaa gatagacttg atgtagaact tgtattttta ttagcagtaa agaacccaga
     5041 ttcacaagta aaactgttaa aggatttaat gtcagttttt caaaatataa agttattaaa
     5101 aaatataaaa aacgcatcaa caaaagaaga agtagctaaa ttattggact ttatagaaat
     5161 ttaatgtagg agaattttat aaataaaaaa ttaaaattta aacaatcaaa gtaagtatta
     5221 tttttttaat aggtttaatc ttatataaaa taaaaattta gagtgaaaaa ttaggaggaa
     5281 attattatga aacaatttaa tgtattatca gtttgtggtt cagggacagt tacatcatct
     5341 atggtagcag gtaaattaaa agaagtacta ggtaataagg ggatttctat tactacaaca
     5401 gaagctagac caacagaagc actaaattta gcacaatcag gaagatttga ctttataaca
     5461 tttacgagtc cacttcaaac aggcgattat ggaataccaa ctataaatgc atttgcttgc
     5521 ctaactggta taggggaaga ggcattcttt gaagaagttt tgactgttat tgaagggata
     5581 gataagtaag ttttggagac gtgaatttat agtatttaaa tcaatgattt agatactata
     5641 ataagctaat attaactaaa tatttgtgga aaaaacattt tatatgttgt cataaattaa
     5701 aaaattataa aaatagtcac ttaagtatta aattaagctt ataaatattt ataagtagga
     5761 gggaaaaaga tgttagaatt tatgaaatct ataatcgata catttggttc tgcaattata
     5821 gttccaatta taatattcat aatagcaaag atatttaaag taactactaa gaaatcgttt
     5881 ctttctgctg tgtatgcagg agttgcttta caaggtttta cactgatttt aaattcattt
     5941 acaccaataa taacaccagt tatcaatagg atggttgaga gtacaggagt aaacctacca
     6001 gtttttgatg ttggttggca agcaacatca ttggttgcat tttcaacaag tgcaggtatg
     6061 atatatttag gtcttggtat attattacaa acaattttat ttttgataaa atggacagac
     6121 gtgtttcaac catcagattt atggaataac tattcttata tggtatgggg agcaatggtt
     6181 ataggagtaa caggaaattt tcctttaggt atagcatgta tggttttatt aaacctatat
     6241 agtttattaa tatcagagtt agttgcaaag agatggtcta gttactatag atatcctaat
     6301 tgtacaataa tagctatgca taatgttgaa gcttctgtgt ttgcagtatt tgcagaccct
     6361 atatacaata aattgggatt aaataaaata aaattaaatc caaaagaatt agagaagaaa
     6421 ttagggtttt taggtgaacc aattacttta ggtctttttt taggtatgtt tataggtata
     6481 ttaggtaata tgacgagaat aaatactatg gaagcttggg gagaaataat gaaagtaggt
     6541 atatctacaa gtgcagtgat ggcaattttc cctaaagtag caagtatgtt tgctcaagca
     6601 tttgcaccta taacagaagc agctagaaag attatgcaaa aagctggaaa ccgtgagtgg
     6661 tacatagcag taaatgatgc tgttggatat ggtgaacctg caactttaat atcaggtctt
     6721 atattaattc caataatgct tgtaatagct atggtacttc ctggtaataa ggtattacca
     6781 gttgtggatt tattggcaat tccatatatg gttcaaggtt tagttgccat acataatggt
     6841 aatataccaa aagtattagt ttcaggtata atttggtttg gattaggatt atatgtttgt
     6901 acatctacag caccattatt tacagatatg gctacaaata taggtgtagc tatacctgca
     6961 ggagcaatgt taataacaag ctttaatata ctaggaaaac ctttgatggg attagttttc
     7021 tttgcatttc taagtgcaaa tccaatttat ataggtcttt cagttgtaat ttactttgtt
     7081 ctatgggcat tgtttagaaa gaacaaaaca agtattcttg attatttaga aaaacaagca
     7141 ctcaaaaatg tagaagaaga gcctgtagca gtttaagttt ataaaaatga aataagaata
     7201 attaaaggag tttataattg attttatata agtatcagat aatacctgag aaatatttaa
     7261 atgaaatata gacttctttt tatttgataa tatttgtaat tataatgcat tgtattttta
     7321 tagttgagtt ttctgtataa cctgctattt aaaacatact cttttatatt tatacagtaa
     7381 tgtggtttaa ttttataaaa tgtaatctct actggtaaaa gactttgtat ataaggcatt
     7441 tgcctgaata aaccatttat cttttgtatg gcatagatat tgctatatag aagtatatag
     7501 catgaaagga gatatttata tgggagataa aatgagagca tcagtattgt ataatgtagg
     7561 tgatgtcaga tatgaaatgg tagatatacc agaaataaca gatacgcaag tgcttgtaaa
     7621 tgtaaaatat gttgggatat gtggttcaga tttacctagg tctatggtaa gtggtttgag
     7681 tggaaataca aaatatccat tgattttagg gcatgaattt tctggtgaag ttgttaaaat
     7741 tggagaaaaa gtaaagcata ttaatgtagg agatagagta gctgttgcac cattagtacc
     7801 ttgtagaaaa tgtgattatt gtaatgaagg taattttggc ttgtgtgatg attacaatat
     7861 aattggaaca agagtaaatg gagcgtttgc agaatatgta agagttccag aagaacatat
     7921 tttaaaacta ccagatacat tggattatga aactgctgcc ggaattgaac cagcaactat
     7981 agcttatcat gggatttcaa agtctaatat tagagtagga gatagtgtag ttgtgttagg
     8041 atgtggtcca ataggtcagt ttgttataca atgggcaaag gtgtttggtg catctaagat
     8101 tatagcagtt gatatttttg atgaaaaatt agaactttct aaacttctag gagcaaacta
     8161 tatacttaac tcaaaagaag taaatgtaat caaagaaata aaaaaaataa caaatggtgg
     8221 tgctgatgtt gtaatagaga ctgctggtag ccgatttaca caagaacaat ctctatttgt
     8281 agcaaaaaag agaggaaata tagtgtttgt aggtatatca cacacagaat taccacttag
     8341 tgcagacgct actgagtgta tattacgtgg agaacttacc ttgaaaggtt cttggaattc
     8401 atatacaagc ccttatcctg gaagagcatg gactgcaaca ttggatttta tgggaaaagg
     8461 tgacattatt tttaaaccca tgatttcaca taaaataggt cttaatgaag ttggtgactt
     8521 tttaagtaaa atgagcaaga gagaaattaa tttcaataaa atcctagtag aaatataaaa
     8581 atttaataaa aaattctaaa agcaattaaa ttttataact atgacattaa ataaaataaa
     8641 aataattttc ttaaataaaa tataggagga agaaaagtgt tagaaaattt aaaagaagaa
     8701 gtattaaaag caaacttaga attacctaag aaaaagctaa ttacttatac ttggggaaat
     8761 gtaagtggaa tagatagaga aaaaggatta gtggtgataa agccaagtgg tgtagagtat
     8821 agtaatatga ctgtagaaga tatggttgta gttgatttag agggaaatat agttgatggc
     8881 aaattaagac cttcttcaga taccccgact catctagttt tatataaaga gtttgaagaa
     8941 ttggggggaa tagtacatac acattcaagt tgggcaacaa tttgggctca aataggaaaa
     9001 tctataccat cttgtggtac tactcatgca gattattttt atggaagtat accatgtacc
     9061 agaaaaatga ctagagaaga aatttgcaat gaatacgaaa aggaaactgg aaatgtaatc
     9121 attgaagaat ttatgggaag aaatccaatt cattgtccag gaataattgt aaatgaccac
     9181 ggacctttta catggggaaa agatgcaaat gaggctgtac acaatgcagt agttttagaa
     9241 gaagtcgcaa agatggctta ttatacagag ttaatgagtc ctgataatat tatggataag
     9301 gttcttatga ataagcattt ttcaagaaaa catggaaaaa atgcatacta tggtcaaaaa
     9361 tgattatctg gaggtgaatt atgctagact ctttaaaagg taaaattgtg gaaatagcaa
     9421 ttaaagcaga gcattatggt ttatgtaaag aaaagactgg taattttagt attagagata
     9481 aggatactgg atatatactt ataaccccat caggtatagg aagagaagaa ttaaaagttg
     9541 aacatatttg tgtattggat ttggatggaa atgtaataga agttgagaaa ggtattaagc
     9601 caagtagtga ggtattgatg catattgaaa tatacaaaac aagagaaaat ataaatgcca
     9661 tatcacatac tcattcttta tatgcaacag cattttcagt agcaaagaaa ccaattctac
     9721 caattgtata tgaatcagtt aattatggcg gttatgtaga tattgctcca tacgaaagac
     9781 caggaacttt aaaattagct aagagtgttt caaaattact gatagataca gatgcatgct
     9841 tattagaaag acatgggttg acaactgtag gtaatgattt agacgaagca ttattaaaat
     9901 caagatatgt tgaagaggta gcagaaatat actataggtc tttagtatta aatcaattta
     9961 aagaacttga agtagtagat gttgatgagt ttaaatcttg gaaataccca gaagaaataa
    10021 agttttaatc taaataattt tcattgaagt ggttaattaa aatatatgta ttatttatat
    10081 caattactat gttttattat attaattacc gtattttgta aatatattta ttacattttt
    10141 tcaaaaaaat atagaatccg aaagccatat aaaattgact tgggattcta tactttttat
    10201 ttatgcattt ttctttatct gattttctgg agaatttata tgttcaaaat attttttagt
    10261 ttcagatact attataggtg ataatgcaag taaagctata aggttaggta ttgccataag
    10321 cccatttaca atatcagcaa ttatccaaac aacttctaac tttaagaaag cacctaataa
    10381 caccatagcc acaaatacag ttctataccc attcatgcct tttacaccaa acagaaattc
    10441 aaaacatctt tctccataat aactccatcc caaaattgta gtaaatgcaa ataatgttaa
    10501 actaactgta agtaatattg gtccaaatgt aggaattgct ccgttaaaag ctgcttgtgt
    10561 cataacagct ccatttaggt ctgaacacca cactcctgaa ataacaagag agaatccagt
    10621 aagagtacag attataatag tatctataaa agtacctgtc atagatataa gaccttgttc
    10681 cgctggccat tcagttttag cagcagcagc agctattgga gcactaccta gaccagactc
    10741 atttgaaaat acaccacgag cgataccatt tctaattgcc agtgacatag ttgctcctaa
    10801 aaaaccacca gtagcagcag taggtgtaaa tgcactttta attattaaca taaaagtttc
    10861 agggatttta ttccaaaaag atattaaaat gattccacag attacaacat atataactgc
    10921 cataaaaggt actattttag tagctacatt agatatactt ttaagtcctc caaatacaac
    10981 caaagctaca aatactgcta atacaacccc tgtaattcta gggtctatcc cgaaactatt
    11041 atttattgca tctgttattg aatttacttg agtaaaagta ccaattccta aaagtgcaac
    11101 tagtatacca cttatagcga aaaatattgc aagaggtctc catttttctc ccataccatt
    11161 tactatgtaa tacattggcc caccagacac ttggccattt ttatctttag ttctgtattt
    11221 tattgcaaga acaccttctg aatacttagt agccattcca aagaatgctg ctatccacat
    11281 ccaaaaaagt gcaccaggtc caccagcttt gatagctgta gcaacaccaa caatattacc
    11341 agtaccaact gtagcagcta gagcagtaca taaggctgca aaacttgaaa catcaccact
    11401 acctttgttt tcagcagaga atattaactt aagagctctt ggcagtttag ttatttgaac
    11461 aactccaagc ttaacagtaa gtagtatacc agtaccaact aatagtacca gtaggggtgg
    11521 tccccaaata aaagcatcaa ctttgtttaa aatatcaatt agattcataa aaacactccc
    11581 tcattttttt tgctttacat aatcaataga atttttaatg aagtaattga caggaaataa
    11641 gtataattta ttaataattt ctagtgtaat aaaaaaagct aaatctaaaa aagattcagc
    11701 tctggatgta cactaattag caaccaaaat aaaagtatta gtaaatactg tttttggtag
    11761 ataatccctg tccttttgcc tgagagattg agcataaaag ccttgcccct tcggcgccca
    11821 taatgggtct ctccagagtt ccgtaaattt tacagtccta cttttaaaag tacctgagag
    11881 tgttactcct tcggtgtgct atatagcaac tcttctataa aatcttaacc ggatattgat
    11941 ttgtcatagc ttattgtatt aataatatta atataagttt ttatgttttg tcaactaaaa
    12001 aattaaaaaa caaagaaaaa atataaaaat gtaagcctat ttacgaatta taaacttaga
    12061 aagatgagta atatacgtga aaatgaattt tgtaatacac atacaaatga agtgtaaaat
    12121 aagttttaaa taaaaaaata ataaaatctt aaataattaa ttagatatga ttaaattatg
    12181 gtaatattat agttacgata ttaagaaaag ttctatttag ttagatttta atcgtaatat
    12241 gagattatgt atagagaaag gatgtatttt tagatgggaa aaatattagt gctagcagaa
    12301 aaaccttctg ttggaagaga tatagcaaaa gttttagggt caaaaaatga aaaaaatggt
    12361 tatatagagg ggccgaagta tgtgataact tgggcactag gtcatttggt tacacttgct
    12421 gaccccgaaa gttatggaga aagatataaa tcatggagct tagaagacct gccaatactt
    12481 cccaagcacc ttaagactgt tgtaataaaa aagagtggaa agcagtttaa tacagttaaa
    12541 tcgcaaatga atagaaatga tatagatgaa atagttatag caacagatgc aggtcgtgaa
    12601 ggagaacttg tagcaaggtg gataatagaa aaatcacagg taaaaaaacc tataaaacgt
    12661 ctttggattt cttcaagtac agacaaagca ataaaagacg gatttgcaaa attaagaagt
    12721 ggaaaagaat atgaaaattt gtattactca gcaatagcac gagcagaagc tgattggatt
    12781 attggaataa atgcaaccag agctttgaca acaaagtaca atgcacagct ttcttgtgga
    12841 agagtacaaa ctcctactct tgcaatgttg cttaaaagag aagaagaaat aagaaatttc
    12901 aaacctaaag agtattatgg attagaatta attgctacaa agggaaattc agatataaaa
    12961 tttatttgga atgataaaaa caataattct tcaactttta gtaaagaaaa gatagaatcc
    13021 acattaaaaa aagtaaaagg tgtagacata aaaatatctg aagtaaacaa gtcatataag
    13081 aaaaaatatt cacctgcact ctatgattta acagaattac aaagagatgc aaataaaatg
    13141 tttggatttt cagcaaaaga aactttatct ataatgcaaa aactgtatga acatcataaa
    13201 gtacttactt atcctagaac tgattctaga tatttaacat cagatattgt tggaacatta
    13261 aaagatagaa taaaagctgt aaatattagt gattattcaa agatttgtaa tagattatta
    13321 aaaagcaata ttatagctaa taaatcgttt gttgacaatt caaaggtaac agaccaccat
    13381 gcaataatac caacagaaca gaaggtattc atgagtgatt ttagtgataa agagcgaaaa
    13441 atatataatc ttgtagtaaa aagattttta gcagtactat ctcctccttt tgaatatgag
    13501 cagacaacta taagtgcaac tgtagaagga gaaatattta aggctaaggg aaacaaagta
    13561 ataaatttag gttggaaaga aagttataaa gatattgaag atgaagatga gtatgaaagt
    13621 ataccaaata ttttagaaaa agatgtactt aaggtcaagg gattaaagat aacttcagga
    13681 aaaacaaatc caccaccata cttgaatgag ggaacattat tgactgctat ggaaaagaat
    13741 ggtctaggga cagttgctac aagagcagat ataatagaaa aattattcaa ttcattttta
    13801 atggaaaaga aaaataaaga tatatatata acatcaaaag gaagacaatt attagattta
    13861 gttccagtag acttaaagac accagaactt acagcttcat gggaaaagaa attattggat
    13921 atttcaaagg gaaaacttag taaaaataca ttcataaatg acatgaaaga ttattcaaaa
    13981 gatattgtac tagaagtaaa aaatagtgat aataaattta aacatgataa cttaactaaa
    14041 aatagatgtc cagaatgtgg aaaatttatg ttagaggtaa atggtaaaaa aggtaagatg
    14101 ttgatttgtg aagatagaga gtgtaatact agaaaaacta tatcacaagt aaccaattca
    14161 agatgtccta actgccataa aaaattagaa cttcgtggag aaggtgaggg gagaatcttc
    14221 acttgtagtt gcggatacag agaaaagttg tcttctttca acaagagaaa gcaagaagaa
    14281 aaacagaagg catcaaaaaa agatgttaat caatatttaa aaaatcaaaa taaaaatcaa
    14341 gagacattta ataatccatt tgcagaagca ttagctaagc taaaaaacaa ataattttaa
    14401 agttattatt atacacaatt ataactttaa ctgtatacta gttatatgta ctgatataat
    14461 atagtgtata gtttaggcat gaaagtaggg ctgttaaatg gatttaagtg gcatatttaa
    14521 atactattgt aaagagtgtg aaaatacatg gaataattcg agtgttgaat tatttgagaa
    14581 tatagaaacg tatagtaaag attcacaaaa aaagagggaa aaagaattag ataaattgct
    14641 aaatacaata tcagttcatt tagagaggta tccaagtgat gctgtattga gaaaaatgtg
    14701 ggtaaaaaag ggcgaggttt ttttacaaaa gacattggaa aaagaaaata tttttaagtt
    14761 agaaaaaatg gatgtagagg atagaaaaaa atttttagat ataacaaaac agtttattag
    14821 agatgctaga aaatttgatg atgatttacc tataggtgat attatgcaag ctatgaggaa
    14881 tgtatggatt tcaaatgcat tacaattatt atttggtaaa gaagtatatt attcaaaagc
    14941 taactttgca tatagtatgt tatatccata tactgataat tatttagaca gtacaaatat
    15001 agataaaaat gataagattt tatttaataa ctggttagaa aaaaggctcc tgggagaaca
    15061 cattaaatct aaggattatc atgaaagtaa agtatctcaa atgatagatt atattgaaag
    15121 tgtataccct agagaaaagt ttacagaagt ttatgaatcg ttattattaa tatttaaaag
    15181 tcaagtaaat agtttaaaac aacatggtaa ggaaaatcat ttgtgtaaag aagatttatt
    15241 atccatttct attgaaaaag gaggttcatc cgttttagta gatggatatt taataagtgg
    15301 attgatgaca aaggaagaaa tagagttttg tataggatat ggatttttat tacaaatatc
    15361 tgatgattta caagatataa aagaagattt aaaatacaac cataaaacta ttattacaga
    15421 gatgtcaaaa gagggtactt tagataaagt tgtaaataaa ctaataaatt ttactattga
    15481 gttaatagat agttttaaaa ttaataataa aaataaatct gtaataacta tgataaagaa
    15541 tgattgctta atgttaattt tattttctgt agtttataat gctgaatttt tttctgtagg
    15601 atatataaaa gaagtagaga aatttattcc atatacaata gattattcat tagagattga
    15661 agaaaaaata aaagaaaaat ttaaaaatat agatgtttta aataatgaaa atgaatataa
    15721 agaaatgatt gatattattt gtgcagagta gaaaaaaagg gagtgtctta gaatagactt
    15781 tttagtttat gaggcactct ttttgtataa aatcaaatgt attttcatca ttttaacaat
    15841 taaaaagaac ttatccctat ttgagaaagc cctttttaat ttaaataaaa atgtcaaggt
    15901 taaactattt aaatgcagta ataatcccat tatcatgtaa gttttttatg attgtagctg
    15961 cgatagggaa tccaaataat ccagctattc caaatagttg agccccaaca aacatacaca
    16021 ttaatgttat taaaggatat aggccaattt ggtgtcctac tacctttggt tctaatattt
    16081 gtctaacaac ggttataatt atgtatagta ttaaaagtcc tattgctaaa ttaatatttc
    16141 cattaatgag tgagataatc atccaaggtg tcaaaatact tcctgtaccc aagactggca
    16201 aaatatcaac aagtgctatg atgacagata tagtaaatgc attttcaacc tttaggatag
    16261 taagtccaat aagtagttct atgaatgtca ctgatagtag tattgcatag gcttttagta
    16321 gttttaaaag agcattaata ctgctatttt ttatatttat tatcataaat tgactttttt
    16381 ctggaaattg ttttaagata aagtttacta ttttttgata gtcaaaagta aagaaaaaag
    16441 aagcaattaa agtaataata agttttaaca taaatgcagg tgttttactt gcaatactag
    16501 caatagcatt gagtgcattt gttgaaatag aagctactaa agacatcata gattgactta
    16561 tattatccca accaagtatt aatgctaggt taacattagg tgtactagca tctatctttg
    16621 aaaataaagt atttagcgtt ggttgtatac ttgatttata tatttcagga agtctaaaaa
    16681 atacatcact tacacttgca aatatcttca caccaaagct aactaataaa aatatacaca
    16741 acccataaaa aattaaaagt attactatag aaataagtgt acgatttaaa tttgtattat
    16801 ttgtaataag tcttataata ggtcttaaga tggatgcaat gataaaactg attacaaaag
    16861 gcattaataa tggcaataag aattttaata caatatatac aatacttgtt attaaaacta
    16921 catatgtaaa tttaattata aaattctttt gcttttgaat atccataata taaaacctcc
    16981 aatgtatttg atattattct aaatatggtc ttttttacag aaaatatact actgtatatt
    17041 gattataaac tataaagctt atgtaaagta aataaattat gtaaaattta ggttaatttt
    17101 gtataaagag atacttttat attttacaat taataaccaa atactaaaaa atattttaaa
    17161 taaagaactt aaaattgtga taaaattaat ttatagatat taaaaaaagt ataatatatg
    17221 gtttttaaga ggtgctaaga tgaaaaattt tagagattta ggtgggaaca aaactgagga
    17281 tggcagaact gtaaagaagg gattatttta taggtctgca aaattatcaa atctaagtga
    17341 aaatgatata aagattttaa aagagttaaa tattaaatat atatttgatt atagaagtga
    17401 tgaagaagct cgtaaacacc catcaacaat aatatcaaat ataaaaaata taagaatacc
    17461 agcaatgaga gagctagaag aatcaggagg aagttttggt tctattgaag atatgattga
    17521 tggattgttt gaaaaagatg gtgcatttaa tatgttaaat aatagttact ataatttgcc
    17581 tataaataat ccgtcatata aaaaattagt agaacttata agagactatt ctaatttacc
    17641 aatattaaat cattgtacag ctggtaagga tagaactggt gtggggagtg ctataattct
    17701 tatgatttta ggggtatcga gagagaatat aatgaaagat tatctaaaaa gtaatgattt
    17761 tgcagataaa gagatagaaa gatttataga atataagcct aaatttaagg acattccaaa
    17821 agaaaatttg aaatatatat ttggtgtaaa tgaagagtat atgaaaactg catttagaag
    17881 aattgatgaa gaatacatta gtgtagaagc atatttatat ggagaattta atttaaataa
    17941 agaagaaata agaaaattaa gaaatcaata tctagagtaa aaggtgagtt tatgagttta
    18001 aatataacag caagacagat aaatataata aaaatattgt taaattcaaa agatacaatt
    18061 agtggtattg ccttaagtca agagatagga tgttcttcaa aaactataca aaatgaaata
    18121 aaggatatta ataaacaact tagaaatggt aaaattttgt caataagagg agttggttat
    18181 aaactagaag gaagttttga tgaggtaaat ctaaatagca atctctatga tgatgtagat
    18241 agagtagaat atataatcag aaaaatatta accttaagta atgaagaaaa aaatactata
    18301 aaacttgaag atttagccga ttctatgtat gtaagtgttt caacagtaaa aaatgattta
    18361 aaagaagtaa aaagaatatt ggaaaagtat tcaatatcag ttaaatctaa gcataaacaa
    18421 ggtatatgtg ttttggaaag tgaagataag atattaaatt gtatagttga cttaagcaat
    18481 aagagagata atcaattgag tttaaatgat tttttaaatg atgatataaa ggaaagaaaa
    18541 ctattaataa aaaaattatt attagacata ttaaatgaaa atggattagt attaactgat
    18601 atagaattta agagtgtgtt aaataatata ttagttatgt taagcagaca taagtataat
    18661 gaaaaagaat ttatagagaa ttatatagaa aattatagga gcaaaagaag ctcaatttta
    18721 gaaaatgaac aaaacaaaga aattataata aattcaataa aaacattttg taaaaattta
    18781 aaattagcta catcaataga tataagtaat gataaggtat ttgaagagtt tttatataag
    18841 catattagta gtttatgtaa aaaaataaag ttaggtataa atcaatcacc tataatggct
    18901 caagatataa aaataaaata tccatttgct tttgaactag ctaatatagc taaaaagaca
    18961 atagaaaaag atttgaagat agaaattaat gaagatgaag ttgcaaacat agccctacat
    19021 ataggaggag cagttgaaag agcatcttat aataataaag ataaggtttt caaaactata
    19081 atagtttgta cttcaggcat aggcacatca atgttaataa aagctaaatt agagaatata
    19141 tttaaggaaa aattagaaat actaaagatt attccttcat acttagttga atatataaaa
    19201 gttatagatg ttgattttgt aatatctact gttcctattg aagtaggaga tgtgccagta
    19261 ataaatatat ctccaatttt aagtgagaaa gaaattagat taattgaaaa atatatagaa
    19321 acaggaaata tatacctaga tttagatata aaaagcctgt ttgatagtga attgtttttt
    19381 actgatttgg tgtttaaaag gaaagaagaa gttatagatt ttatggcaag taaacttgta
    19441 caaaagggct atatagatga ggatatgaaa aaatcttact ttgagagaga aaaaatagca
    19501 acaacagaaa tagggaatat ggtaactata ccacatggag ctattggaaa aatttttgat
    19561 aataaaatag caattggtat attaaagaag cctatttctt gggaaatatc agatgttcgt
    19621 ttaataataa tgttagccct tgataaagat aaaatattag attatgaagt aatattttca
    19681 aaaatatata aaagagtaga ttctatagca aaagttataa gtatatgtga aaatagtagt
    19741 tttgaaaaat ttgtaaattt atttaattag atgggtagta ttgtatatac acctgtaaat
    19801 ttggataata taaaactaaa taagataaaa acgataaatt tctcttttaa ttaagagaaa
    19861 ttttttgact ttcaaggata ttaatataaa gttaagatat aaacataaag caaataaata
    19921 aaaaagaggt ggcaatatga tttatacagt gacattaaat ccatcaatag attatatagt
    19981 taagctagat gagctaaaga caggaagtac aaatcgtgta aaagaagaat acgtttatcc
    20041 aggaggaaaa ggtataaatg tatctcgcat acttaaggaa cttggaaatg ataatacatc
    20101 tttaggtttt ataagtggtt ttactggaga atatataata agaacattgg aagaaaaaga
    20161 attgaagaca gattttataa agataaaaag tggtttttca agaataaatg taaaaataaa
    20221 ggcgttaaag gaaactgaga ttaatggaca aggtccaaat atagatgatg aagacattga
    20281 tactttatat aaaaaacttg ataagctaaa tcaagatgat attttaatat tggcaggaag
    20341 tataccatct actttagatg aaaaactata cgaaaatata atggctagat tagaaaaaaa
    20401 gaatataaaa gttgtagtag atgcaactaa aaatcttctt cttaatgttt taaaatacaa
    20461 accttttcta attaagccta ataacgacga attagaagaa ctatttggtg ttaaattaaa
    20521 ttcaattgaa gacatggtaa aatatgctag aaggttaaag gaaatgggag ctataaatgt
    20581 cttggtatca atgggaaaag atggagcatt gttgattaca gaggaagatg aagtgcttat
    20641 aagtgatgtt ccaaaaggaa aagtaaaaaa ttctgtagga gcaggtgatt caatggtagc
    20701 aggatttata tctgggtatt tgaatactgg gaaatatgat tatgctctta aattgggagc
    20761 agcttcaggt agtgcaactg ctttttcata tgacttagcc aaaagagaat acattgataa
    20821 attggtaaat gaaatatctg taaagaagtt ttaaaatatt aaaaaatata tagaaaggta
    20881 attaatattt atgaaaatta tagatttatt agatgaaaag tcaattaagt taaatttaaa
    20941 ttcaaaaacc aaatcagaag caatagaaga attagtagat ttggtcgcaa atagtggaaa
    21001 tttaaatgat aaagaaaact acaaaaaagc aattttggct cgtgaagaaa tgagtacaac
    21061 aggaattgga gaaggtgtag ctatacctca tgctaaaaat tcttcagtta ctaaagcttg
    21121 tatagcagca gcagtgtcaa aagaaggtat agattatgaa tcgtttgacg gaagtttatc
    21181 aaatctattt tttatgatag ctgcaccaga cggagctaac aatactcatc ttgaagtatt
    21241 atcaagatta tctacaatac ttatggatga agactttaga aataaactta taaattcaag
    21301 ctctgaaaaa gaatttaagg aaataataga taaaaaggaa agagaaaaat tcagtgaaga
    21361 atatcaagat gagaaagtgg ctgaaaaaaa tataatagat actaaagaga atgatgctaa
    21421 taagtatcct aaggttttag cagtaacagc atgtccaact ggaatagcac atacatttat
    21481 ggcagctgaa agtttaaata aaatggcaga aaacaaaggt gtatcaataa aagtagaaac
    21541 taatggttca gcaggtgtta aaaataagct tacaaaagat gaaatagaaa atgcaacatg
    21601 tataatagtg gcagcagata aaaatgttga gatggctaga tttaatggca aaaaagtcat
    21661 aaaaactaag gtggctgatg gtatacacaa agcggaagaa cttatagaca aggctgtaaa
    21721 tggagatgct ccaatttacc atggaggaga tggaagtcac aacgaatcaa atgaagaaag
    21781 tgaaagtgga tttagaaaag tatacaaaca cttaatgaat ggtgtatcta atatgttacc
    21841 atttgttatt ggtggaggga tacttatagc gatagccttt ttacttgatg attatactat
    21901 taacccttca aactttggtt caaatacacc tatagcagca ttttttaaag gaataggaga
    21961 taaagctttt ggatttatgt tacctgtact tgctggatat atagcatact ctatatctga
    22021 tagaccagcg tttgttgtag gatttgtagg aggtgctcta gcaggtgatg gtggttcagg
    22081 atttttaggt gcactacttg caggttttat agcaggttat ttagtagaag ggcttaagaa
    22141 aatatttagt gtactaccag cttcattgga aggaataaaa ccagtattgt tatatccttt
    22201 acttggaaca cttttgatgg gaattataat gacattttta gtaatacctc cagttacagc
    22261 aattaataat gcaatggttg gatttttaaa tggtttaggt ggaacttcaa aaatattttt
    22321 aggattagta ttaggtggaa tgatggctgt ggatatgggt ggacctgtaa ataaagcagc
    22381 atatgtattt ggagttgcat cacttgaatc agggcagttt gaaataatgg cagcagtaat
    22441 ggctggtggt atggttccgc cacttgctat agctcttgca actacatttt ttaagaatag
    22501 atttactaaa gaagaaagag attctggtaa agttaattat gtaatgggat tatcatttgt
    22561 tactgaagga gctatacctt ttgcagctgg agacccactt catgttatac cagcttgtgt
    22621 tggaggttca gcagtggcag gagcacttag tatgttgttc aatgcagctc ttcgtgcacc
    22681 tcatggagga gtatttgtta taccagtggt tacccatcca tttgcatata tattagctat
    22741 agctgtaggt gctttggttg gaatgatgct tttagcatta cttaaaaaac cattaaatca
    22801 agaagtatag caattaaagg atattcaaga acttaaaata tgatgtttta taaaaattaa
    22861 tatattgctg aattatttta aaataaggat tttatgaggc tgttatataa cgatttgtaa
    22921 ttgttatctt gacagtcttt tatttttata taagtaaaat aagtgtgttt tgctttattt
    22981 gtatttaaat tgtttttaag ttagaatata aagaaaatta tata
//
LOCUS       DAGSWI010000060        22260 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.060, whole genome shotgun sequence.
ACCESSION   DAGSWI010000060 DAGSWI010000000
VERSION     DAGSWI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.060"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            166..945
                     /gene="hisJ"
                     /locus_tag="MJF80_002104"
     CDS             166..945
                     /gene="hisJ"
                     /locus_tag="MJF80_002104"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBZ0280765.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGEELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNKILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLDLVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(1258..2139)
                     /locus_tag="MJF80_002105"
     CDS             complement(1258..2139)
                     /locus_tag="MJF80_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBZ0280766.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKISEG
                     EIELPDPIIMENDKLMK"
     gene            2692..3204
                     /locus_tag="MJF80_002106"
     CDS             2692..3204
                     /locus_tag="MJF80_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280767.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLNDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            complement(4029..5126)
                     /locus_tag="MJF80_002107"
     CDS             complement(4029..5126)
                     /locus_tag="MJF80_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBZ0280768.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            5419..6945
                     /gene="glpK"
                     /locus_tag="MJF80_002108"
     CDS             5419..6945
                     /gene="glpK"
                     /locus_tag="MJF80_002108"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBZ0280769.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            6986..8101
                     /locus_tag="MJF80_002109"
     CDS             6986..8101
                     /locus_tag="MJF80_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBZ0280770.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(8382..9146)
                     /locus_tag="MJF80_002110"
     CDS             complement(8382..9146)
                     /locus_tag="MJF80_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBZ0280771.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            9405..9495
                     /locus_tag="MJF80_002111"
     tRNA            9405..9495
                     /locus_tag="MJF80_002111"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9439..9441,aa:Ser,seq:gct)
     gene            9583..10371
                     /locus_tag="MJF80_002112"
     CDS             9583..10371
                     /locus_tag="MJF80_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBZ0280772.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            10392..11132
                     /locus_tag="MJF80_002113"
     CDS             10392..11132
                     /locus_tag="MJF80_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBZ0280773.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEAGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            11308..11676
                     /locus_tag="MJF80_002114"
     CDS             11308..11676
                     /locus_tag="MJF80_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280774.1"
                     /translation="MGNKNTKYKDKTRERYNKKVENLNKEKLNKVNKQSKKVDKIDDI
                     INKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVKAL
                     FSIFVIVCFLIAFKYVVNPV"
     gene            11768..13228
                     /locus_tag="MJF80_002115"
     CDS             11768..13228
                     /locus_tag="MJF80_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBZ0280775.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            13281..16310
                     /locus_tag="MJF80_002116"
     CDS             13281..16310
                     /locus_tag="MJF80_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBZ0280776.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            16387..18453
                     /locus_tag="MJF80_002117"
     CDS             16387..18453
                     /locus_tag="MJF80_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBZ0280777.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    19129..19339
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            19565..20380
                     /locus_tag="MJF80_002118"
     CDS             19565..20380
                     /locus_tag="MJF80_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBZ0280778.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            20408..21085
                     /locus_tag="MJF80_002119"
     CDS             20408..21085
                     /locus_tag="MJF80_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBZ0280779.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            21144..21866
                     /locus_tag="MJF80_002120"
     CDS             21144..21866
                     /locus_tag="MJF80_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280780.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         8823 a         2293 c         3840 g         7304 t
ORIGIN      
        1 tgttaagttg aaaatagtac taatgataag atacttaaaa ggcagtttaa aattactcta
       61 atattagata taataaaaat tagcatgatt aaaaatatta ttttgaagtt aaaagattta
      121 tgcatttata ttagattttt tctttaaaaa aggagtgaat tatttatgaa agatggtcat
      181 atacattcac cttattgtcc tcatggaagt aaggatgatt ttgaaaaata tgtacaaaga
      241 gcaatggatg ttggaataac agaaatgaca tttacagaac attttccgta tgcaaatgga
      301 tttagagacc ctgctcctga aaatgatagt tgtatgagta ttgaagattt accaaagtat
      361 tttaatgatg tcaagaagtt gaaagaaaag tatgagggta aaatcaagat aaatgttggg
      421 tcggaagtgg attttataga aggatacgaa gaagggataa aggagaattt aaataaatat
      481 ggagaagaat tagaggattc tcttttatca gtacatataa ttaagattga tgatgattat
      541 tattgtgtag attacagtgt agaggagttt caaaatttaa tcgacaaatt aggaagtatt
      601 gaagctgttt ataataagta ttatgaaaca ttgataaaag ctgtaaattc agatttaggt
      661 atacataaac caaaaagaat aggtcattta aatttagtta gaaagtttaa tcaggtattt
      721 ccatacgatt ataaaggaaa taaaattctt gaagagttaa taaagttaat taaagaaaaa
      781 ggttatgaac ttgattataa tgtttctgga aatagaaaag agtactgcaa agagccttat
      841 atagatggat acttattaga cttggtaaaa aaatacgata ttccattagt tttaggttct
      901 gattctcact gtgcagagga aataaataaa tacgatatta tttagatata ttcacacaaa
      961 agactgtaga taagtttgta taaaataaat ttatgtacag tcttttgtaa atttatatga
     1021 ttgaaaatag taatagaact taaacaaaat aataataagt tctaagtaaa ataacaatag
     1081 aatctaaata aaataataat aaaattcaat agaataatag gacttagaat taatctatct
     1141 tatttatata aacattctta tataagattg tagataagct tgtatttaca caaatctata
     1201 gaccttctct attatattga cttagtaatt ataaatacta ttttgcgttt aaagttatta
     1261 tttcataagt ttatcgtttt ccattattat agggtcaggt aattcaattt caccttctga
     1321 gatcttcaat ctattaaaca tataattttt tacatcttct ttatttttaa atttagtaac
     1381 ttgataaggt gatgtaacac catatttttc taaaaataga atgccatttt tagttttaat
     1441 acatatgcct gtatgaccta taaatacatt tttagtttct ggataacgaa gaaatccatt
     1501 tataatagat atatttttat tatttttaaa agatattttt ctttttttcc attcattttt
     1561 tatttgttct gcaattttga ttgtatcatc tgtatcttta gctggtattg cagaaaagaa
     1621 gttgataaat ttttctacat ctttggtgct aaatttagca tctttattat tcattatagt
     1681 gtccaagtcg acattcaaat caatagagtc tccagtaaat ttcttatttg aatttataaa
     1741 atctttaaaa agtctaaaag cagttattct acaatttaca tccatataat cagggaattt
     1801 tttcacccaa ttatcttgca atttttctag gttatatgga acttgttttg cattgattga
     1861 agaatatcca gatttagata tatttatatt attttgaagt attttaactt gcaatttatt
     1921 ataattgttg actgctttaa taaatgtatc tatatctttt tcattaattt tattttcttt
     1981 taggatttcc tttacactat tttgagtttt tttatctatc atattagaat atatcaattt
     2041 attattatta tacttaatat ttgaatatgc aaatatcgta agtatacttg atagtactaa
     2101 gacaacagac aatatcatta ttattaatct ttttttcata tttagttctc ctttttaaat
     2161 ttattgtgtc gacatattta tagtatatag tacctttaaa tttaccttca atatgttttt
     2221 aattacaaaa ctgttgtaaa aaatataact ttatttaaaa ctttttttta atatgtaaat
     2281 atatatacta atagggggta cttattttta gaatattcag taaaatgatt ttgatatact
     2341 ctctatattt tgtatgttaa aagtaaataa attattgtta aagaaggtaa agacttttaa
     2401 atttcttatc ataggatttt aagtaaataa gaaggattgg agtaattaaa aatttttatt
     2461 ttgcaaaaat aagtatttat ttatatggat aaattagatt ttaaatctat aaatatagat
     2521 aaaatctaat tatttaactt caactattcc tcaaaaaagc aacttttaag gaatagttga
     2581 agcaaaaagt ttggttacat aatagtaatt ttagttacaa gaatgaaact gtaaaactta
     2641 ggattttctt tatataattt cattgagaac atttaattta ggaggtaaga tatgaaagta
     2701 aaaaaattta aaagtggtat agtaatggca ttaacagtaa tggcaattgg agcatcattg
     2761 acaaatgtat cagctatgga attaaacaag agtaaagagt ttgaaactaa atacaatgaa
     2821 atgaagaggg attttcaaga ggaatatgga aaagaagaca aatttaatat aacattgaat
     2881 gatgaattta caaaagaaga agtaaatgga gctaaaggga ttataaagac aactaataaa
     2941 atgaataatg aggttgtagt attaaattat tttgatgaaa tgaaatctgg aattgaattt
     3001 gagttgaagc caaaagcaga aaaaaatgca attttgagag atgaatttca acaaggttat
     3061 ggaaaggaag ataaattcaa agttgtgcta gatgatgaat ttacaaaaga agaggtaaat
     3121 ggagctaagg gcattataaa gactacagat aaagagacag gtgaggttat cgtatataat
     3181 tactttgatg agcttgaaaa ataatgctta aagattactt tgattgaatg aaaaggtaat
     3241 tgtgttgaga atattaaatg actgaataaa aacagttgta tcaaaacaaa caataataga
     3301 atgtaagaat gattgtgtcg aaaatataaa atgactgaat aaaaaacgat tgcgtcaaaa
     3361 ataaacaata atagaatgta agaataatta tgtcgaaaat ataaaatgac tgaataaaaa
     3421 cagttgtatc aaaacaaaca ataatagaat gtaaaaatga ttgtgtcgaa agtattaaat
     3481 gactgaataa aaaacgattg cgtcaaaaat aaacaataat agaatgtaag aataattgtg
     3541 tcaagaatat taaatgactg aataaaaaca gttgtatcaa aaacaaacaa taatagaatg
     3601 taagaatgat tgtgttgaaa atattaaatg actgaataaa aaacgattgt gtcaaaaaca
     3661 aaaaataatt aagtgtaaga atgattgtat caagaatata aaatgattca acaaaaaacg
     3721 attgcgtcaa aaataaacaa taatagaatg taagaatgat tgtatcaaga atataaaatg
     3781 atttaacaaa aaacgattgt gttaaaaata aaaaacaatt aaatgtaaga ataattatgt
     3841 caaaaatata aaataaatta aaagaaaaag attatactga aggaaaaaga tttgaaatag
     3901 gggtgaactt tacatattat agttcaccct tatacaaaaa tataaaatat aatttattat
     3961 aggaaaattt aaaaaaatca gtctatttaa tttatactga tatattgtag ggttttacaa
     4021 acaaactgtt atataacttt ttttatctcc aattttgatt ttaagatatc taagaattta
     4081 taacaatctt ctggatatat ttctcccata tttgctactt ggaatatgtt agcatctagg
     4141 aatttgcctt ttcctggata gacaacatag ccactttccc tttctagccc taatataaac
     4201 tcatttacat tgatttcttt aggtagaaat acagatgtta ctgtattaga catatactta
     4261 tctggaagta aaaatgttag acccaattct tccataccat tccttaaaat agcagaacat
     4321 tctttgtatc tttctatttt actgtcatct tccaaaaact cagttaaagc ttcattaagg
     4381 gcaaatatta aagtaacatt aggtgtattt ggtgtttgag aagatgattt agcaacttta
     4441 tagtgtttat aaagattaag atacacattc ttacattgat tttctttaat attttgtaaa
     4501 atactctctt tagcacaaag gtatgcagaa cctggaaatg ctccaagagc tttgccacca
     4561 acacctgtta ttatatctat attaaactct tccacatcaa tatattcacc tgctgaggca
     4621 cttattgcat ctacaaaaaa tattttgtta tacttcttag caagttttcc tagttcaggg
     4681 actggattta tcattcctga actagtttca tggaacacca tagcaattac ttttatatca
     4741 gaattttcaa ttatgtattt ttctaaaatg tctaggtccg ggtattctgc ccattcaaaa
     4801 ttaggtttat atgtttttac attataagta gataatattt cttctagcct ttctccaaaa
     4861 acaccattac taactagcag aactgcatct tctttgtcaa agatagaaga tagtacacat
     4921 tcatttacag atgttccaga gccagataca attaatgagt agtaattaga agttgcatta
     4981 tatagtttat tgattttttg ttgtgtatct ttgaataaat ccataaactc atttcctcta
     5041 tggcatatat catagtgaag aagtgaattt ctgactttat ctgtaaccat tactggtcca
     5101 ggactgaata gatatgtttt tttcattgta aagcctcctt aaaaagttaa atttaaattt
     5161 ttgaatgact gattatgtgt aaatattaaa atgttatgtg ttattttaac tagattatag
     5221 aataaaataa taaatattgc aataacattt caataaaaat acttttttaa tcactataat
     5281 taaaaataac aaaaaatatt gtaatgaata ttgcataaat ccacatgatg atataaaata
     5341 taaataactt gaatagatta tatattcaaa aaataaaaaa gaatttatta attctgaaaa
     5401 taaaggggga catttaaagt gaaatatgta ttagcaattg accaaggaac aacaggaaca
     5461 agagcagtat tgataaacga aaaatcagag tttgttttta gcgattatat ggaacatact
     5521 caaatatatc cacaatctgg atatgttgaa catgacccta tagaaatttg gaataataca
     5581 aaaactgtga gtaataatgt attacaacag gcttttgaaa atggaatcaa tgaaagtgat
     5641 ataaaaggaa ttggtataga taatcaaggt gaaacagtaa tgttatggga taaaaataca
     5701 ggtaaaccac tttacaatgc tattgtgtgg cagtgcagga gaacttatga ttatgtggaa
     5761 aatttaaaga atataccagg tttagaaaat aagataatag ataagactgg gttaattata
     5821 gacccatatt tctcaggtac aaaaattaaa tgggtaatag ataatgtaaa aggtgtaaaa
     5881 gaaaaaatta aaaatggaga agtacttgca ggaacattag atagttggtt aatatggaaa
     5941 atgacaggag gaaaatcttt tttaactgat gtatctacag cagcaagaac aatgcttttt
     6001 aatataagag atatggattg ggatagagag ttgcttgaca ttataggagt acctagaagt
     6061 attttagcag atataatgcc tacgagtggt gattttggaa ctacagataa ggaggcattt
     6121 tgtggggttt ctataaaaat tactgcttca atggtagacc aaccagcagc tttatttggc
     6181 aatggatgtt ttgaaccagg aatggttaaa aatacttatg gtacaggatg tttcctatat
     6241 atgaatatag gtgaggaact aaatatagca ggtaatggta ttttgacaac tgttgcttgg
     6301 aaaataggag ataaggttac atatgcttat gatggtggag tgtatatagc aggtgctgca
     6361 atacaatggc ttagagatgg cattggagtt ataaaaaatt attcagaaac tgatgacatg
     6421 gcaaattcca tatcaagtac tggaggtgta tattttgtac cagcatttgc aggtatagca
     6481 gcaccttatt gggaccaata tgctagaggt acaatggttg ggataacagg aagtactaaa
     6541 aaagagcata tagtaagagc gactcttgag tctgttgcac ttcaagtcaa agatgttgtt
     6601 gattctatga acttagcctc agggaaaaaa ataaaaatgt tgagagtaga tggtggaatt
     6661 actaaaaata actttacaat gcaatttcaa tccgacatat taggtatacc aattgaaatt
     6721 gcagaaaatc ctgagacgac agtacttgga gtaggatata tggcaggtct ttcttgtggt
     6781 gtctggaaca gtattgatga aattagaagt gaatttacaa taagcaaaaa atatattcca
     6841 aatatgacta ctcaaaaaag agaagaaata ttacttggat ggaaccaagc agtaaaaaga
     6901 gctatgaatt gggaaaacga taccaaaaag gtgtgtgcta attaagttac aatagagtat
     6961 tttacatgta taggaggaat taaaaatgcc ctttgataaa attatattat acataatggc
     7021 tgtgggagtg cttttaggag cgttagatag aataattgga aataaatttg gacttggtga
     7081 gcagtttgaa gaagggttta attcaatggg tccattggct ttaggaatgg ttggtatagt
     7141 ttgtttagct ccagtaattt cagatgtact aggtcctata ataataccaa tcttcaatct
     7201 ctgtggagct gacccatcta tgtttgcaac tattttagcg aatgatatgg gagggtatcc
     7261 attggctatg gaacttgcac acaataaaga ggcaggaatg cttgcaggat tggttgtgtc
     7321 atccatgctt ggatgtacat tagtattttc aattccagta ggactaggtc ttatagaata
     7381 tgaagacaga ccattctttg caaaagggtt actgataggc ttgataacaa taccttttgg
     7441 aggtattata ggaggattaa tagcaggatt taacttatca atggtaatta taaatatgat
     7501 accagtgata atattggcag taatattggc agtaggttta aaatttaatt ctacgcttat
     7561 gataaatggg tcattagttt ttggaaaatt tatatctata ttgataacga taggtttggg
     7621 agcagcagca tttcaagaaa taacaggtat agtacttata aaaggcatga cacctataag
     7681 agaaggtatt caagtaattg gaagtattgc aatcgtgtta ttaggtactt ttccaatatt
     7741 acatttatta gttaaagttc ttaataaacc acttacaata gtgggtggaa aattaggtat
     7801 ggatgccact agtgcagcag gattagtttt tactttagcc aatagtattc cagtatataa
     7861 aatgatgaaa gatatgagtc caaaggggaa agtagtaaat actgcttggc tggtttgtgc
     7921 tacagcagct ttgggggacc atttaggatt tacagcagga gtagttccag agatgattac
     7981 acctgttgta attggaaaat tggttggagg agtattggca attgcacttg ctatacttat
     8041 gacaaaagat ttaaccttgg aagaagaaca aagtattaaa atgaagaaag atgcagtgta
     8101 gaataaacaa tacttaagtc aaatttaaat tattttaaaa taggagttta aataactaaa
     8161 aataaattaa aaagacagac aattaaaaaa taatcgaata actaaaactt ataaattaaa
     8221 taaaatggca atcaaataac taaaaatgat aagctaactg aaagtagtta cataattaaa
     8281 atataaagta tagtttagac ataaatggag ctaaataaat aattatttgg ctccttaatt
     8341 ttaatttatt acatcaagga actaaaaatc aatcatataa gctaattatc agctatgact
     8401 aaaggtattc ctttttgaga aaactctatt attatattat ggtctatact agcagaggta
     8461 ataatagtat ctatatgttc tataggagct actgatgcaa atgaatttat accaaactta
     8521 ggaggttcga caactactat agattttcta gacctttcaa tcattttttt tctaacaagt
     8581 gcttcttcta agtgatagtc agagattcca ttttcaagag tgatacctcc agcgcctaaa
     8641 atagctttat caacattaaa ttgttcaata gctgacagtg taagcggacc agaaaaagaa
     8701 ccttcagttt ctctaagata gcccccaagc acatatactt ttatattagt attcataagt
     8761 tcgtttgcaa ctaaaattga gttggttatt acagttagat tctttttatc tttcaaattt
     8821 tttgaaactt caagtacagt agttccagta tccataaaaa tagtatcatt tgtatttata
     8881 aactcactac atttaatagc aatctgtttt ttgtcgtttg agtttatttt ttttctttta
     8941 acatagttaa gttctacgct acttgctata actggtatag caccaccata aactcttttt
     9001 aaaagaccga tactctccat atgggctaaa tctcttcgtg cagtttctat tgatatatca
     9061 aacatttgaa ctatgtcatt tattttaatt atattatctg atttgagaat acttaaaatc
     9121 ttctcatatc tttcctgtgg taacataaaa tatcctcctt ggactagtat tattttagtt
     9181 ttggcataac ttacaattaa gtcagagttt aggtatattt gttaatgtat gtatatttat
     9241 caaataatat tataagtgtt atgcaagata attttaccat atttaaaact tacatttaag
     9301 caaaaaagta aaaatagatg tttaataatt caaaataact caaattttat aaaacaattg
     9361 actaaaatat gattttatta tataatttac tagtatagag atatggagaa gtactcaagt
     9421 ggctcaagag gatcccctgc taagggatta ggctgggtaa ccggtgcgag ggttcgaatc
     9481 cctccttctc cgccaattat taaaggctat ttttattaaa gatagtcttt ttataatgtc
     9541 ataaatgatt agatatgtat aactaaagaa aggagggaat agatgacaac tttaattttt
     9601 ttcatagtgg ggatagcatc tttggtagtt gcttttgtta ttgatggggg acatgttgga
     9661 gcattatttg tattttcatc agcacttgtt gtgtttggtg gaactatagg agctgttgga
     9721 gcttcgactc cattccctgt gtttaaaaag tctataaaga taatgttatt agcttttaaa
     9781 aataaaaagc ctaataacat agaaaatata atctatttca aagaaattgc aacaaaggct
     9841 aggaaagagg ggcttcttag tattgacaaa gaaataagtg agaatgatga attagaccct
     9901 tttattagaa aagggcttga acttatggta gatggagtgg atgcagttac tataagaaat
     9961 atattggaat cacagataca gataacatca aaacgacata aagaaggaat agcaattttt
    10021 gattcagcag gtggatatgc acctacactg gggataatag gtacagttat gggattagta
    10081 catattttag gtaatctttc aagtaatcca gatgcattag gacctcaaat ttctgttgca
    10141 tttttggcta ctttgtatgg tgttggttct gccaatttaa tatggttacc aatagcatca
    10201 aaacttaagg ctataaatca acaagaagta agtgaaagac ttttggtgat agaagcaata
    10261 ttatccatac ttgagggtga taattctaat ttaatgacag aaaaattaaa aatattttta
    10321 aattcaaaag aactaattga acttgaagaa attggtggta gagctgaata atataaaagg
    10381 atggaatgtt tatgttgtat gatgaagatg aaaaagaaga aaacaatgag cgatggcttt
    10441 taacttattc agacctcata acattactta tgattttctt tgtaataatg tattctatga
    10501 gtaatgttga tgcggagaaa tataaacaat tatctcaatc tctaaattct gcttttggtg
    10561 gttcttctgg agttattgaa ggtggtaata gcaagataga accagtagtt gaagcaggaa
    10621 gtaatgattt agatacttta caaaatgcta agtttaaaaa aattggtgaa gaaatccaga
    10681 agtatttaaa cgaaaatggt atggctaatt cagtatcatt aagagttcaa gatagaggat
    10741 tagtaataag ccttaaagat acaatactat ttgatacagg gaaagcgatt gtgaaggata
    10801 attctagaga taagattatt caaataggta agatgttaaa tgaaatgaat agttacatga
    10861 gagtagaagg ccatacagac aatatgtcta taaaaaattc agagtttaag tcaaattggg
    10921 atttatcagt tatgagagca accaatgttg ttcaacttct tattgacaat gcaggtatag
    10981 caccagataa attatctgct gtaggatatg gtgagtttag accaatagca gaaaattcat
    11041 ctgaagaagg taggtcaaaa aatagaagag ttgatatagt tttagttgat agtaaatatg
    11101 ataatgtaga aaatgtttca aaggataaat agttaaatta gctgtctcaa tacagaatta
    11161 attctaaagg agatggcttt tttatgcgaa aatcaaaaat agatacatag taagttacct
    11221 ttttgtagga atatatggta tcttaaatat ataatattta aataatgtat aagctcaatt
    11281 aaaaaattta tagaggatgg aaaataaatg ggaaataaaa atactaaata taaagataaa
    11341 acaagagaaa ggtataataa aaaagttgag aatttaaata aagaaaaatt aaataaagtt
    11401 aataagcaat ctaagaaggt agataagatt gatgatatta ttaataaatc tagtattgta
    11461 ttatgtgtac ttttagtagt gttttcatta ctttacttat ttaaactggt tcaacttaat
    11521 ataataatga tacttttagt aattacagta gctattcaac aaggattggt gtcatttggt
    11581 gcatttagaa ataaaaatta tgtaaaagct ttattttcta tatttgtaat agtatgtttc
    11641 ttaattgctt ttaaatatgt tgtaaatcct gtttaaatat atcaataaac gttctataat
    11701 taatataaat agtgaagtta cagttttaat gctattataa attttataat ttgaaggagt
    11761 agattttatg aataggtgta agtttgcaaa attaattatc acatcaatga ttgttttatt
    11821 acttacagga tgtagtttaa agaaggaaga taattataag ttagacccag aaaatccgac
    11881 atcaatacaa atttggcatt attataatgg acagcagaaa atggcttttg ataagcttgt
    11941 taaggaattt aatgacactg taggtgctga aaaaggaatt attgttgaag cattcagtca
    12001 aggtaatgta gttgatttat cagagaaagt tttagattct gcaaataaaa aagttggttc
    12061 tgaagatgta ccaaatatat ttgcagcata ctcagatact gcatatgaaa tggataaatt
    12121 agggttacta gtagatatgg ataagtacat aagtaaagaa gaattaaatg aatacataga
    12181 cgaatatgta gaagaaggca gaataaattc taaggatgag tttaaacttt tcccaattgc
    12241 aaaatcaact gaaattttga tgttaaataa aactgattgg gataaatttg caaaagctac
    12301 aaattcaaat ataaatgata taaaaacaat tgaaggatta gtagagacat caaaaaaata
    12361 ttatgaatgg acagatagtt taacgaaaga gcctaatgat ggtaaagcat tttttggaag
    12421 agatgcttta gcaaattata ttattatagg aagtaaacaa ctaggagtag aaattttttc
    12481 tgttaaagat ggtaaagtta cactaaatat tgatgaaaaa gttatgaaaa aactatggga
    12541 taatttctat gtaccatata taaatggata ttttgcttct tatggtaagt ttcgttctga
    12601 tgatgcaaaa acaggtgata tattagctct tgtaggttct agttcaggag cagcatactt
    12661 tccagataga gtaatgctaa atgataatga gagttataat attgaaacat ctgtttttca
    12721 agcaccacgt tttaaaggag aaggaaaata tgctgtacaa caaggtgcag gaatggctgt
    12781 aacaaaatct actaaagaag aagaatatgc atcagtagaa tttttaaaat ggtttacaga
    12841 taaagaacct aatataaaat tctctattgc ttctggatac ttaccagtaa agaaaaaaac
    12901 gaatgatttg gatgttataa aagaagttat gaagaaagat aatttgagtt tatctgataa
    12961 agttgaaggt tcaatagcct catctattga acagattaac agttgtaatt tatatactaa
    13021 taaagcattt gatggtggaa ctaaagctag acaaatactt gaatattcaa tgcttgataa
    13081 agctaaagaa gattcaaata aaataaatga attagtaaaa agtggaactt ctaagaagga
    13141 tgcagtagca caatttaata atgataaaaa ttttaaaact tggtttgaaa gttttaaggc
    13201 tgagcttgaa aatgctataa aaaaatagat gaattggcag ttagtgtgtt gacgagcaca
    13261 aaggtatagg tggaatgggt atggaaaaca atctaagaaa aaacaattca ataatgaaaa
    13321 agatgatatt tcccctaatt attgttatgc taattcaaac aagtttattt tgtgccacaa
    13381 ttttatgggg aggaactatt aagaaattaa atgataattc gtttgacatc ttaaatgaaa
    13441 gagtaattaa tcgtaaaaat tatatacaaa atgaaatgtt acaaagatgg tcaaatacaa
    13501 gtgaaacgga agctacaatt aattcctctg taaaaaaagt attaaatgaa aaaaatgcct
    13561 taattgagga tgtaggcaac aatgaagaaa ttaacaaaga tattataaaa agcatatcaa
    13621 atgatttaat atatcttttg agaaaaaatt ctgttacagg agcttttata atcctaagtg
    13681 acaaaaatat taacgataag tcaatagata gtaaaggtga aataagtagg acaggattgt
    13741 atataaggga tttagaccct aagtttaatc caaatgataa ttctgatttg ttaattgaga
    13801 gaggttcatc agacatagca agaagttttg gaatatctat ggacagttac tgggaaccta
    13861 agttttcatt taaaagtgaa aatgagcaag atggagatga ttttttttat aaaccattta
    13921 gagctgcaat tgataatcca aatgtatcaa gttctaattt aggatactgg ggaagtaagt
    13981 ttttattgac aaatgcaggt gataggctta tgacatatac agttcccctt atttatgaag
    14041 atggaactgt atatggagta cttggaatag atttaagtgt aaattattta agagaattac
    14101 ttccatattc agagataaat gggaataaaa agggtaatta tattttaggt gtagatagaa
    14161 ataatgatat ggcctttgaa aatgtaattt caaatggata ctcattaaaa gaaaaatttt
    14221 gggatggttt acattctaat ttagagcaga aggaatcgta taaaaatata tataagatta
    14281 aaaataaaga taatgaatgt ggtaatggca acatttatgc tagtgtacaa tacttagatt
    14341 tatataactc caatactcca tttgaaaatg aacgttgggc tcttttgggt attattgaag
    14401 aaaaaagtct tttgtcattt tcaaaaaggg tagagaaatt agttataata tctatagcaa
    14461 tttctttatt aataggtcta ataggtattt acattgcatc aagattattt acaaaaccaa
    14521 ttgttctttt agccaacaaa gttagaaaca gtaatccatc aaaaccagta aatcttgata
    14581 agataaatat atctgaaata gatgaattat cttcagctat agagttgctc agtgcaaatg
    14641 ttgcagattc ttcgtcaaaa ttatcacaga ttattgaaat ggtagatatg ccaattgggg
    14701 cttttgagtt atacaaaaac gcagaaaatg ttttttgtac aaaagaattt tttagcgtgt
    14761 ttactactga aatcaaattt gaagaatatg gatatatgga aaaaaataag ttcctgaaaa
    14821 aattaagggg actagataaa tatttgcaag aaagttatga aacagaaaat acatatatat
    14881 ataagtttga atctaaagat aaaaaactta agtggataag aatgaatatc tttgaagatg
    14941 agctaaaggt tcttggtgtt gttgttgatg taacaaaaga atttattgag aaaagaaaga
    15001 tagaatatga aagagatttt gatattttaa cgaatctttt aaataggaga gcttttcatt
    15061 cacagataca agaaaaattt aagaataaag atgaacttaa gatagcagca tttattatgt
    15121 gggatttaga taatctaaaa tatattaatg atacatatgg acatgatgct ggtgatgaat
    15181 atattaggtg tgctgcagac atacttaaga agtttatatc atggaatggt atagtatcta
    15241 gactttcagg tgatgagttt tatgtattta tatatggata tgaaagtaaa gatagtataa
    15301 gagatattat taattctgtt aagaaacaga tgaataatac cttattaaaa cttcatgatg
    15361 gaacagaatt taaaataagg gcatcagcag gaatttcctg gtaccctgat gattcaacca
    15421 actatgatga attaatgaga tacgcagatt ttgctatgta tcaaattaaa aatacaatca
    15481 agggtgaagt tagtgaattt gatattaata tatataataa ggattcattc ttattgagta
    15541 atagagaaga attaaacaag ttaattgatg gtcaattagt tgagtatgca tttcagccta
    15601 ttgtagattg taaaggtaaa gtttttgcat atgaagcact tatgagacct aaaataaaaa
    15661 cttttagttc accaatagat gtcattagat tagctcgttc acagtctaaa ttatatcaaa
    15721 ttgagcgtat tacatggttt aaagcactta aatcatttaa aaaatacaaa gaaaaatttg
    15781 gcgattgcaa actgttctta aattctattc ctaataataa tttgtcaaaa gtagattttg
    15841 aggaaattga agatttatat ggtgaatatt taaatagagt agtactagaa ataacagaga
    15901 atgataagat taatgaagat tttattgaga aaaagaaagc atatatagac cattggaata
    15961 tagaaattgc tttagatgac tttgggacag gttacaacgg agatgctgta ctattatata
    16021 taatgccaga ctatgtaaag attgatatgt ctattgtaag aggtattgat aaagatgaaa
    16081 acagacagaa aatattaaaa aatcttatct catattctaa cgaaagaaat attaaagtga
    16141 tagcagaagg tgtagaaaca aaaggtgaaa tggaaacact aattaatctt ggagtagatt
    16201 atatgcaagg ctactatatt agtaaaccat catttatacc tgaagatata agcgacaaaa
    16261 ttaaaaaaga actcaagata tgtcatgaaa ataggaatga tgtggtttaa tagaggaaaa
    16321 attaaattta tttttatgaa cttttagttt ataattaaaa agaaaataat ataattataa
    16381 atcaaggtgg tggagctaat aatcacaaca aatatagata taagaaagtt aaatgaaaat
    16441 caattagaag cagttgagca tatagatggg ccttgcatga tacttgcagg gcctggttca
    16501 ggtaagacca gagtaattac atatagaata gccaatatgg ttgtaaacaa aaacatagca
    16561 ccaacaagaa tcttggcaat aagttttacc aaggcatctt caatagaaat gaaaaataga
    16621 gctttgaatt taagtgatga tataagactt aataaggtaa cctatggtac ttttcactct
    16681 gtatttttta aaattctaag atactttgaa agatacaact tagacagtat atttgatgaa
    16741 aagtcaaagc gaatgacaat caaggcaatc ctaaaaagtt taaatataga gaatgctgat
    16801 gatgatgaga atataggaca agttataaat gaaatttcct atgtaaaaaa tgaacttatg
    16861 gataaaaatg aatttaattc agaagtatta acaaaagatg agtttttaaa agtatacaac
    16921 ttatacgaag aacaaaaatc taaagttaat aaaatagatt ttgatgatat gcttataaga
    16981 acttattatt tacttttgaa caataagtct gcactagaaa tggttagaaa tgtgtataaa
    17041 tatatattga tagatgaatt tcaagatata aataaagtac agtttgaagt tttaaagtta
    17101 atttgtagcc cattaaacaa tatatttgca gttggagatg aagaccaaag tatatatgga
    17161 tttagaggtg ctagaccaga ttttttgctt gaatttgaac aatactttaa taatactaaa
    17221 aaaataatat tagatataaa ttacaggtct aaaagtgaga tagtgcatac tgcaaataga
    17281 cttatcgaca aaaataaaaa tagatatgaa aaaatcatta agtgtagtca gggggatgga
    17341 ggaagtgtta cctatatttc tccgcatgat tcagaggaag aagccttata tattgccagg
    17401 gaaataattg acgaaataca aaaagattat gtagagtatt cagattttgc agtaatttat
    17461 agaactaata tacagtcaag agcattagta gatgtattta tggatatgag gattccattt
    17521 gtagtaaaag actcagtaat tacaatatat gaccattggg caagtcaaga tatactagcc
    17581 tatttaagaa taggtattaa tccaaaatca aataaagatt ggttgagaat tataaacaag
    17641 ccttttagat atatatccaa agacagtata aatatggtta aggatgaaaa agactttata
    17701 acagcattaa taaataagtg taatcttcat cctaaacaag ttaaaacaat aaatgatttg
    17761 gaaatagatt taagttattt aaatacttta aatcctaaaa atgcaatatc ttatattaga
    17821 actagtcttg attatgatag atatgtttta gattattgta gcaacagaaa gataaagact
    17881 aatggacttg tagagatatt aaatgaactt gagagttcag caactaattt taatactgtt
    17941 acagagtttt tagagcatat tgatagagta aagactgagt taacggaaaa taacaagaat
    18001 aagcagactg agggtgtcat atttacaact atgcatagtg caaaaggatt agagtttaga
    18061 aatgtttata ttataggtgt aaatgaagga acaatacctc atgaaaaatc atatgatatt
    18121 tgtaaggaag agaaaaaaga agaacaatta gaagaagaaa gaagactgat gtatgttgct
    18181 attacaagag ctgaagaaaa gttatgtata agctctactt taaataagta tggtaagaaa
    18241 gttgataaat cattatttat taatgatata aaatctccaa cgaaaaaaga aattgacagt
    18301 attggtgtag gggataaggt ataccataaa aaatttcatg aaggagaaat aattaaaaaa
    18361 gatggaatta tgtttacaat aaagtttaaa gatagagagc gaatcttgga tttgaaaact
    18421 tgtttattga agaatataat ttatactatt taatttagaa tctaaattta gatagaaata
    18481 taatgtatta tttattatgt gcaagacaaa tagatatgtg taaaatgtat tatttaactt
    18541 attagaaaaa agaaataagg gtttaatata ttatttatag ttgtttagaa aaagcaacaa
    18601 tgtggaaaat attatttagt attattaaag aaaagcaaca atgtgaaaga tattatttag
    18661 tgtaatttaa gaaaaagcaa cagtgtgaaa gatactattt agtattatta aagaaaaagc
    18721 aacagtgtga aagatattat ttagtgtaat ttaagaaaag caacaatgcg aaagatatta
    18781 tttagtattg tttaagaaag ggcaacagtg tgaaagatac tatttagtat tattaaagaa
    18841 aaagcaacag tgtgaaagat attatttagt gtaatttaag aaaagcaaca atgtgaaaga
    18901 tattatttag tattgtttaa gaaaaagcaa caatgtgaaa gatattattt agtattattt
    18961 aagaaatagc taaaaatatg aaatatacta tttataatta cacaaataat atcaataaat
    19021 ataaacagta gtatttagat aatttacaag taaaaattat taaaaaaata aaaaacttat
    19081 tgacaaaaat attataagga agtacaattg tattaacaat ttaaggaaac aaatccaatg
    19141 atagagaaag tagcatatat ggatttacag cgagttaggg gttggtgtaa gcctaacaat
    19201 gaagtctatg tgaagagagc tctggaggag attatcagaa actttagtat gataattggt
    19261 ttacccgcct taagggattg agaggataag tacttactta tcaaaaagag tggtaacgcg
    19321 gatataattc gtctcttagc tgtaaagcta agggactttt ttgattttaa aataaaaata
    19381 tccatacagc ttaaaagcta acagtcacaa attttgaaag taatataagt atttatcaat
    19441 aagagtggta acgcggtaaa gtcgtctctt agtcattaaa gactgagaga ctttttttgt
    19501 aactcgaaac atttaagtat taaaatctga gggaaattat aattaaaaga gaggggtagt
    19561 taaaatgaaa gtatttaaaa aattattaag tttaggatta gtattaggat tgacattatc
    19621 gctagtagga tgtagtggtg gaggagaaaa aacaaagtta gaacaaataa aagacaatgg
    19681 aaaattagtt gttggtacta gtgcagaatt tcctccattt gaatttcata aggtagtaga
    19741 tggaaaagat tcaataaaag gattcgatat aatgttggct gaagaatttg caaaagagct
    19801 tggtgtaaag gttgaaatta aggatatgtc atttgatgga ttaataggtg ctttaaatgc
    19861 agaccaagtt gatattgttc ttgcaggcat gtctccaaca ccagagagag aaaagagtgt
    19921 tgatttttca gaattatatt atttaagtag aaatgcagtt atagttaaag atgcagatat
    19981 agacaaagta aaaactgaag atgatttaaa gaaacttaga gttggagttc aagcaggaag
    20041 tatccaagaa gaatatgtag ttaacacttt aaaaatgaca actacaaaat ctttgaaagc
    20101 aatacctgat ttaattacag aattaaaaaa tggaaacata gatgcagttg ttactaatga
    20161 agcagtttct ttaataaatg ttaaaaaata tgatggaata aaaatggcaa atactgaagt
    20221 tgggaaagat gtaacagaag gtatggcagc agcaatcaaa aaatctgaca ataacaaaga
    20281 ttttatagaa ttattaaaca aaaagataaa agaattacaa gatggcaaga agatagaaga
    20341 atttttaaat gaagcatcta ctgaagctgc ttcaaattag taagaaagtt atgtgggaga
    20401 tgaaattatg agtttagatt ttagtttttt aagtagattt gggacttctt ttttggaagg
    20461 aacaggtgtg acagtatcaa tttctcttgt ggcattatgc tttggattta taataggtat
    20521 aattatctgt atggcaaaaa tatcaaagag caaagtatta agagcaatat catcaatcta
    20581 tattgaggtt ttaagaggga caccattatt agtacagata tatatagtat ggtttggttt
    20641 acctcaatta ggaataagat tccctatgtt gtttggtata ccaagtgaat ttatagctag
    20701 tgcatttgca ttatcagtaa actctggtgc atacgtggca gagatactta gatcaggtat
    20761 acaatcagtt gataatggac aaatggaagc aagtagatca ttaggattaa attattggtc
    20821 aactatgaga tatataataa tacctcaagc tataaaaaat atattacctt cattagcaaa
    20881 tgagtttatt acacttgtaa aagaatcttc aataatatct gtaataggag ttgttgagat
    20941 aatgcgtact gctgatatag taaaaaatgc agcatttaga gcattagagc cattaatagt
    21001 agcagcagca atttactttg ttataacatt tactctatca agattagttg gattattaga
    21061 aaagaaacta tctgtgtcta actagatgag agcatgttta gcatttaggg aaaacataga
    21121 atagtaaaaa ggaaagtgat aatatgatta caataaaaaa tttaagtaaa tcttttggag
    21181 atttaaatgt attaaaaaat atagacttag aaatcgctaa aggcgaaata atggttatag
    21241 taggtccaag tggttctggt aaaagtacac ttcttagatg tatgaattta ctagaaatac
    21301 ctacaggtgg agaaataata tttgaaggaa aaaatttggt cgataaaaaa actaatattg
    21361 atgaggttag acaaaatata ggtatggtgt ttcagaactt taatttattc ccacataaga
    21421 ctattttaga taatataaca ttagcaccaa ttaaattaaa aaagatgact aaggaagaag
    21481 cagaaaaaaa agcagaaatt ttgttgagta gagttggtct tttagataaa aaagattcat
    21541 atccatcaca attatctgga ggacaaaagc aaagaatagc aatagctaga gcacttgcta
    21601 tggaaccaga tatgatgttg tttgatgagc caacatctgc tcttgaccca gaaatggtaa
    21661 acgaagtttt ggatgttata aaagaacttg ctaaagaagg tatgacaatg gcaattgtaa
    21721 ctcatgagat gggatttgct aaggaagttg cagatagagt tatttttata gatggaggaa
    21781 gtatacttga agataatact ccagaagaag ttttcggaaa tccaaaacat gaaagaacaa
    21841 aagcattttt agcaaaagtg ctataattat ataaaaagga tgttattgat attagaaatt
    21901 caatacatcc ttttttagtg atggttaata ttaattttaa aattaaagtt atattaatat
    21961 taactttatt tttacgattt tttgcagaaa aaaaaagagc tcggggtaag ctcttttttt
    22021 ccaccgaatt aatttccgtt actaatccga tttttaataa atataaaata tatccttatg
    22081 aaagggtatt gggaatagat atattaacta actagggtta ccagggggat aaccttaatt
    22141 tattataaca aaatgcgaca caaaaatcaa tatttttttt gaaataaatt ataaaagtta
    22201 tttttataac aagtaagctt ataaatttaa cttttatagt aaaaataagt atatgtataa
//
LOCUS       DAGSWI010000061        22224 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.061, whole genome shotgun sequence.
ACCESSION   DAGSWI010000061 DAGSWI010000000
VERSION     DAGSWI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.061"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            68..304
                     /locus_tag="MJF80_002121"
     CDS             68..304
                     /locus_tag="MJF80_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBZ0280781.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            317..1660
                     /gene="yqfD"
                     /locus_tag="MJF80_002122"
     CDS             317..1660
                     /gene="yqfD"
                     /locus_tag="MJF80_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBZ0280782.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            1599..2714
                     /locus_tag="MJF80_002123"
     CDS             1599..2714
                     /locus_tag="MJF80_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBZ0280783.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            2771..3232
                     /gene="ybeY"
                     /locus_tag="MJF80_002124"
     CDS             2771..3232
                     /gene="ybeY"
                     /locus_tag="MJF80_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBZ0280784.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            3238..3957
                     /locus_tag="MJF80_002125"
     CDS             3238..3957
                     /locus_tag="MJF80_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBZ0280785.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            4076..4477
                     /locus_tag="MJF80_002126"
     CDS             4076..4477
                     /locus_tag="MJF80_002126"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBZ0280786.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            4541..5434
                     /gene="era"
                     /locus_tag="MJF80_002127"
     CDS             4541..5434
                     /gene="era"
                     /locus_tag="MJF80_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBZ0280787.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            5446..6825
                     /gene="mgtE"
                     /locus_tag="MJF80_002128"
     CDS             5446..6825
                     /gene="mgtE"
                     /locus_tag="MJF80_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBZ0280788.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            6898..7053
                     /locus_tag="MJF80_002129"
     CDS             6898..7053
                     /locus_tag="MJF80_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBZ0280789.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            7019..7792
                     /gene="recO"
                     /locus_tag="MJF80_002130"
     CDS             7019..7792
                     /gene="recO"
                     /locus_tag="MJF80_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBZ0280790.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            7785..8384
                     /locus_tag="MJF80_002131"
     CDS             7785..8384
                     /locus_tag="MJF80_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBZ0280791.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            8544..9422
                     /gene="glyQ"
                     /locus_tag="MJF80_002132"
     CDS             8544..9422
                     /gene="glyQ"
                     /locus_tag="MJF80_002132"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBZ0280792.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            9422..11488
                     /locus_tag="MJF80_002133"
     CDS             9422..11488
                     /locus_tag="MJF80_002133"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBZ0280793.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKILIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(11654..13201)
                     /locus_tag="MJF80_002134"
     CDS             complement(11654..13201)
                     /locus_tag="MJF80_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBZ0280794.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            13481..14248
                     /locus_tag="MJF80_002135"
     CDS             13481..14248
                     /locus_tag="MJF80_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBZ0280795.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            14531..14728
                     /locus_tag="MJF80_002136"
     CDS             14531..14728
                     /locus_tag="MJF80_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBZ0280796.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            14757..15833
                     /gene="vorB"
                     /locus_tag="MJF80_002137"
     CDS             14757..15833
                     /gene="vorB"
                     /locus_tag="MJF80_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBZ0280797.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            15836..16555
                     /locus_tag="MJF80_002138"
     CDS             15836..16555
                     /locus_tag="MJF80_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBZ0280798.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            16568..17107
                     /locus_tag="MJF80_002139"
     CDS             16568..17107
                     /locus_tag="MJF80_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBZ0280799.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            17264..18322
                     /gene="buk"
                     /locus_tag="MJF80_002140"
     CDS             17264..18322
                     /gene="buk"
                     /locus_tag="MJF80_002140"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBZ0280800.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            18340..19233
                     /locus_tag="MJF80_002141"
     CDS             18340..19233
                     /locus_tag="MJF80_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBZ0280801.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            19263..20471
                     /locus_tag="MJF80_002142"
     CDS             19263..20471
                     /locus_tag="MJF80_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBZ0280802.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            20495..21754
                     /locus_tag="MJF80_002143"
     CDS             20495..21754
                     /locus_tag="MJF80_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0280803.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT         8712 a         2429 c         4134 g         6949 t
ORIGIN      
        1 gttacaacat caataaagtg cataaatacc tatttcaagc ataaaattaa ttaagggggt
       61 aatgcttatg ttagaaatat ccactgattt acaagtaaat caaccagtta ttactgttac
      121 aagtaatacg tttattagta tagaaaatta tctatcaata ttagaatatg aagttgattt
      181 aataagaata aagactaaag ttaaaacaat taaaatatct ggggataaac tttcacttaa
      241 gtatattact gatagtgaga taggtataaa agggattatc tacaatgttg agtatgttga
      301 ttaggaggag atacacttga taagttttat aaggggatac tatgttatag ttgtggaagg
      361 agtaggactc gaacaatttt taaatcatct tataagaaat gggataagtg tttacaatgt
      421 aactagaata aaaaatacta aaatggagtt tcatatagat agacaagata taaaagactt
      481 taagaatgtt tatagaggaa gtaagtttga tataaaggta aagcaaaaaa ctggggtccc
      541 ttttattatc aaaagagttt ataagcacaa aggtatgtgg atttgtgctc ttgtttcttt
      601 atttttattg atgtctacat cgcagtttgt gacagatgta tatatacaat ctcctgaagg
      661 tataaaaaaa gaagctctta gaaatgagct ttataaggtt ggagtaaggc caggagtcta
      721 taagaagagt attgatagaa aagaagttag agaccatatg atgtcaaaat ttaatgatgt
      781 ggcatattta tctatcaatg taaaaggaac aaatatattt gttactgtta caaaaaaggc
      841 agaatcccta aaatctacag accaatcaaa ttattgtaat gtaattgctt taaaaaatgg
      901 tataatagaa aaggtaatcc ctagaagtgg taaatcagtt attaaatctg gagacatagt
      961 ccaaaaggga gatgtcttat taaatggagc taataccaag tcaataccag aagtatgggc
     1021 aagtactttt tatgagtcca caaaaaaggc aagttatgta gatactgtaa ataaaaaaac
     1081 tggagaaaag aaaaatatat atacactaag tttttatgat aaagagttta caatgagaaa
     1141 aaatataaaa tataaagatt atgttgttga aaataaagaa aaaaaactat caataggaaa
     1201 ctatactttt ccaataaaga taaaaacgag tactttttat gaaacaaaaa aggttgaagt
     1261 aaaaaggaat aaagaagagt taaaaaaaga attatctgaa aaagctttaa aagagttaga
     1321 atacataatc ccagcttcgg ctagaataat agatgtaaaa cataacttta aggttaataa
     1381 aaatatgtta gaatatttaa taacagttca aactagtgaa aatattgcta aaatttatcc
     1441 tcttagtaag tcagaagcag aaaggtttat aaaggaagaa agtaaaccag atgaaggtga
     1501 agaggaagtt ccttccaatc cagaaaaaag accattaaat gatataagaa atgagtttga
     1561 tgaggataat aaggataata aagaccaaaa caattctgat gaaaataata gtaatcaaaa
     1621 tagtaataat agtcaaaata ataatagcaa taacaactaa taaaattaaa cttaaaatct
     1681 taggaggact aattcaattg ataatacaaa agaaatttac aatagcagat ggtaactttg
     1741 aaagagagtt atttggcaac tttgatgaaa atgtaaaatt aatagagaag actttaaata
     1801 tagatgtaat acttagagaa ggcaatataa ttttaattgg agaagaaaaa aatgtagatt
     1861 ctgcattaaa acttatgaat gaattacacc aaactgtatc aaatggtaag catttagata
     1921 agcagagtat ttcttattca ttatctcttt tgcttgaagg aagtgaacaa aaaataaaag
     1981 aacttgaggg gacaatcgtc ataactcaaa ggggaaaagc tgttcaacct aaaactttag
     2041 gtcaaaaaga atatataaaa cttatagaaa ataatgatat aacttttggt gttggacctg
     2101 ctggaacagg aaagacttat cttgcggttg ctatggcagt aaaagctttt aaaagagatg
     2161 aagttagtag aattatactt acaagaccag ctgtggaagc aggagagagt ctaggttttt
     2221 taccaggaga tttaaaggac aaagttgacc catatttaag accactatat gatgccttat
     2281 ttgaaatgtt gggtgctgat aaatttaata agtatttaga aagaggtact attgaggttg
     2341 caccacttgc atttatgaga ggtagaacac ttgataattc atttataata ttggatgaag
     2401 ctcaaaatac tacttctgaa caaatgaaaa tgtttttaac tagacttgga tttggctcta
     2461 aagctgtagt tacaggagat gtaactcaaa cagatttacc acaaaataag aagagtggtc
     2521 taatacaagc tacagagatt cttaaaggtg ttccaggtat tgggtctata atgctaacag
     2581 atagagatgt tgtaagacat gagttggtac agagaataat aagagcttat gagaagcacg
     2641 acaaaagaga agaatttaaa aaagaagaac gtaaaaaagc gaaaatgcag gaaaagactt
     2701 tcaaaagaaa gtaaaagtgt agtaatatag atatatagca ttattaatga ataaaaagta
     2761 ggtgtagtga atggatttga tactagatga tagacaagat aaattagaag taagtgagga
     2821 gttaattgaa aagataaagg atataatcat agaatgttta gattatgaag gatatgatga
     2881 taattatgaa gtgagtcttt cttttgtaga taataaagag attcatgaat taaatagaga
     2941 atatagagga gttgatagag tgactgatgt tttatcattt cctcttttaa gtgatgattt
     3001 tgaggatgtt gagttagaag aagaatccct aggtgacata gtagtatctc ttgaaagagc
     3061 cttagaacag agtatagagt ataaccatag ttttgaaaga gaagtatgtt ttttaatatg
     3121 tcatagtatg tttcatcttt taggttatga ccacgataca gatgaaaata caaaagaaat
     3181 gagagaaaaa gaagaacaca tattaaataa gttaaacata acaagggagt agtttttatg
     3241 aaaccagaaa aaactcgtca aggtataatt aaagcattta atgcagctat agaagggata
     3301 ttgtatacat ttaaatttga aagaaatatg aaaatacatt atttaggttc agttgctgtt
     3361 cttataataa gtttgttttt taacttttca aaattagaaa tgataatgtt acttatgtcc
     3421 atatgtttag ttgtagttgc tgaaatgttt aatacagcta tagaaaaagc tgttgattta
     3481 gtaactgatg aatatcatgt tcttgctaag atagcaaaag atgttgcagc tggaggcgtt
     3541 ctagtggcag cactaaattc agtagtagtt ggatatattt tgttttatga taagttgaca
     3601 gatatatctg gaatattaat ttataaaata agagaatcag aattacatat aaccttgata
     3661 tgtatattat tggttcttat agctgttgta gttgtaaaag cattgacttc tacaggtact
     3721 cctctaaaag gaggaatgcc aagtggtcat gctgccttag catttgctat agcaactgca
     3781 attacactta tgactgaaag agtagttgcc tctactcttg catatataat ggctgtatta
     3841 gttgcccaaa gtagaattga aggaaaaata catacctttt gggagactat tgctggagct
     3901 ttgttaggtg tgttgatagc tatattagta tttcagcttg gaatgttcta taattaaaat
     3961 aaatgtacat aaaatattta aaataaaaca aatctgtgct aattaaattt ttatgatata
     4021 atgtagagta ttaaataaaa ataactttta attattgtat acaggaggag ttgttgtgga
     4081 taacatagaa ttgttaaggt tggcagaaga tgccagacaa cattcttatg ctccttactc
     4141 aggttttaga gtaggtgcag ctcttttaac taagagtggt aaagtttata ctggatgtaa
     4201 tatagaatgt gcatcattag gtggaacaaa ttgtgcagaa agaacagcta tttttaaagc
     4261 aatatcagag ggtgataagg atatctataa aatagctata gcaagtgata actcagaaaa
     4321 taatgagcaa acatatccat gtggaatatg caggcaggta attatagagt ttggttctga
     4381 tataaaaata ataacgggat atactaaagg agaggtgttt gagcatagta taaaggattt
     4441 gttgccaaac tatttctctg gaaatgattt aaaataaatt taaaagtata aataaattta
     4501 agtaattaaa acaagaagat aaaaagttag gagacagtta atgttcaaat caggttttgt
     4561 cagtatagtg ggtagaccaa atgtaggaaa atctacttta atgaataatg tagttggaga
     4621 aaaaatagct ataatgagtg ataagcctca aactacaaga aatacaatac aagcagtata
     4681 tacagatgaa gaaatgcaga tagtattctt agatacacca ggtattcaca aaccaaaaaa
     4741 taaattaggt gaatttatgg taaaggcagc aacagaagct tttaaaaatg tagatttaat
     4801 attatttgta gttgatgatt ctaaaaaaat aggtcctgga gatagaaaga taatagagga
     4861 tttaagaagt gttaagacac caataatttt agttgtaaac aaaatagacc aactagacca
     4921 aaaagatgag ctatttgata taatcaaaat gtatgataga gaaggcattt ttaaagaaat
     4981 agtaccaata tcagcattaa aaggaaagaa tacggatacc ttaataaaag ttatacaaaa
     5041 ttatttagaa gaagggccaa agtattttcc agattatatg attacagacc agccagaaag
     5101 agtattaata gcagaactta taagagaaaa agttcttcac tatcttaatg atgaaatacc
     5161 tcatggtgta gctgttgaaa ttgaaaaaat gaaagcgaga aatgataagg aaatagtaga
     5221 tgtatctgct gtaatatact gtgaaagaga ttcacataag ggaataataa taggaaaaaa
     5281 tggtaggaaa ctaaaaggaa taggtaagtc agcaagacaa gatatagaat tgcttttagg
     5341 ttcacaaata aatcttcaat tatgggttaa agtcaaagaa aattggagaa atttacaaaa
     5401 ttatataaat aattttggat ataatgataa ataaaggtgg gttatatgga taggaaactg
     5461 gatgcttccc aagatttatt atatgaagta aaatcattaa ttgataataa taaagtattg
     5521 gagttaagag aattaataga ggaatatcat ataattgata tattcgatat aatggaaaat
     5581 ttagaggaag acatgaaaat tcaattattt gaagtacttc ctttagatat ggcatcttct
     5641 atattagaag aaggtagtgt agagtttttt attagtattt tatctaaact ggatgtagaa
     5701 cactcgaaaa atatacttga acttatgtct cttggagata tggcagacaa gttgagtgaa
     5761 cttgaagaag aagaaagaga acacataata aatcttttga atcaagagaa tgccgattat
     5821 gtaaaagagt tactattcta tgatgaagat tctgctggtg gaacaatgac cactggatat
     5881 atttctataa ataaagatat gacagctctt gaggctatag accatatgag agaagaagct
     5941 gaagaagctg aaactattta ttatatatat gtggttgatg atgaagaaaa actagttgga
     6001 gtattgtcat taagagaatt aataatagct agagacgcaa atatagtaga agatttgatg
     6061 agtgaaaata taatctctgt ttatgtagat gaagatagag aagaagcagt aagattagta
     6121 tctaaataca atttaatagc aattccagta gtagacagac aagaaaaatt aaaaggaata
     6181 ataacagtag atgatataat tgatgtaatg gaagaagaag caactgaaga tatgtataaa
     6241 tttgcaggaa gttcagaaca tgaaagagaa gttgctgaaa aagaaaaccc tacattgaga
     6301 gagcaaataa tatcagcact tagaggacga ttaccttggt taattattac cttagttgga
     6361 gggttgttgg cttcattaat actttctaac ttagactata taatgaatcc tgtatatgct
     6421 ccattagtat tttttatacc tgtagtaatt ggtatgggtg gaaatattgg aactcaatca
     6481 tcatctgtta cagtaataac attatctaat aaggatttaa attttagcaa tgtggttaga
     6541 gaaggtattg taggtattat tacaggtctt ttgtgtagta taataacagg tattgtaatt
     6601 tactttgtta tgagaaagtt agacattgta ttaattgttt caatatcatt atttataaat
     6661 atggttttag gtgcaacaat aggtgcgttt atgcctgttt tattaaaaaa aatggatgca
     6721 gacccatcta cagtttcatc accaattata tctacagctc ttgatataac tggtatagca
     6781 gtttacttta taataacaac ggcattattg tcaaaaattg tttaataact ttatgtatga
     6841 tttatcctct tcttactaaa aatataagta taaatgtaag aagggggttt tttaaaaatg
     6901 aacaatatca gttgggaagt ttttaaaaaa acaggtagta tagatgcata tttatatttc
     6961 tgtgattgca aaaacataca tgaagaaatt gaagaagtaa gggagaaaaa agaagacgat
     7021 gataatcctg aacacccagg ggatagtact taaagctata aggtataagg aaagtgacat
     7081 aatacttaca ctatttacaa gaaagcttgg aaaagtatct gcaatagcaa aaggagctaa
     7141 aaaaaataag agctctttgc tttcatcatc acagttattt tcttatagca attttacctt
     7201 aaaaaaacaa ggtaatatgt acaaggttac tcaaagtgaa ataataaaaa gtttttataa
     7261 tatatcctat gatatagagg cattttctta tgctacatat attacaaaat tagttgaaaa
     7321 ttctatactt gaaaatcaga ctaataatag gctatttata ttacttgcgc agactctgta
     7381 tttgtataca caggataata cagacaatag gtttataaca gcagcattcg agttaaaatt
     7441 tttagactat ataggattta aacctatagt aaataaatgc attaattgtg aatgtaaaat
     7501 tttacaaaat tctgtgttta atatctacga aggtggtata ctatgtagta agtgtagtaa
     7561 actttttgat aataatataa aattagactt aacaactata agtcttatgg aatatgtatt
     7621 aagaaatgat attttaacat gtagtaaagc taaagtatca aaatatataa cacatgaact
     7681 tgagaatatt ctagacaagt atttgaaagt ttatgttgat aatattaatt ttaaatcatt
     7741 acacgtattg caaagtgtaa aaaataataa gggagtggat aatgatgagt agtaatataa
     7801 ctattgaaat ggttgattca gtctttgaaa gagttccagg agcaacttat gcagaagcaa
     7861 aagaagcatt agtaatgtgc aatggagatg taattgaggc agtaatatat ttagaaaata
     7921 aaaaaaatac ttgtaattgt aaagcaaaat ctgctaaaga aactatggaa gaagtatttg
     7981 gcaaagatgg agaaaatata aagtttcaat taaaagagct tcttagaaaa agtagtgtag
     8041 tgagaataat agttgaaaaa gatggaaaaa ctatgatgaa cattccttta acagttggag
     8101 ttgttggatt agcttttgta ccattagcta ctttagttgg attatcagct gctgttataa
     8161 gtaaatatcg tataaaagtt caaaatgaag aagatggaga aattgtagat ttaggtgaat
     8221 tgaatgaaga aaaacttcac gttttgaaga atatgataac taatgcagca aaagatgtga
     8281 aggatgtagt agttgacaaa aaagaagatg acaaagatgt aactgctgac cttatgaaag
     8341 aagaatctga aaagaataaa gaagacgaag acgaaaataa atagatataa agtgactttt
     8401 aaaaaatgaa gagaagataa ttataagaat tatcactaac attgacaaat tttatagaat
     8461 ttgatattct aaaagttgta gaagaactat taaaatagcc tttcgtgtag acgagaggct
     8521 ttattgttat aggagggatt tttatgaatt ttcaagagat gatactggcg ttgcaaaaat
     8581 actggtcaaa gcaaggttgt atcatgatgc aaccttatga tattgaaaaa ggtgcgggaa
     8641 ctatgaatcc taatactttt ttaagatcat taggacctga gccttggcaa gtatgctatg
     8701 tagagccttc aagaagacca gcagatggaa gatatggtga aaacccaaac agactatatc
     8761 aacatcatca attccaagtt atactaaaac cttctccaga taatatacaa gaattatatt
     8821 tggaaagttt gaaagagatt ggaattgacc caagtgagca tgacataaga tttgttgaag
     8881 ataactggga agctgctaca gttggtgctt ggggacttgg atgggaagtt tggctagatg
     8941 gtatggaaat aactcaattt acgtattttc aacaagtagg taatattgaa tgtgagcttg
     9001 agacaggaga aattacatat ggattagaaa gattagctat gtacattcaa gaagttgata
     9061 gtgtttatga tttaaaatgg aatgataaaa taacttatgg tgaagtattt aataaagctg
     9121 agtatgaaaa ctcaatgtac gcatttgaat tatgtgatgc agatatgttg tttaatctgt
     9181 ttgatatata tgaaaaagaa gcattaagac ttatggaaaa tggccttgta ataccgtcat
     9241 atgactatgt gttaaaatgc tctcatgcat ttaatacatt ggatgcaaga ggtgcaatag
     9301 gagttagtca aagagcttca tttataggta gagttagaaa tatggcaaag actgttgcag
     9361 aaacttttgt aaaacaaaga gaagaaatgg gattccctct tttaaaggac ggtgacaaat
     9421 aatgaaaaat tatcttttat ttgaaatcgg tgttgaagaa atgccatcaa gatttgtagg
     9481 cagtacacta gaacaattaa aaaacaatct tagtaaatta tttgatgaaa atagaatagc
     9541 ttttgataaa ataaatgctt atgggacacc aagaagactt actttagtag tcgaaggatt
     9601 gagtgataga caaagtgatt tagaagagga aataaagggt cctgctaaaa aaattgctgt
     9661 tgatgcagaa aataatttaa caaaaccagc attaggattt ataaaaagca aaggattaag
     9721 cgaatctgac atatatttta aacaagttgg aaaagaagaa tatatatttg cgactataaa
     9781 gcaagatgga aaagaaactt cagaagtact aaaagacata ttaccagaga ctataaaatc
     9841 tgtagtattc ccaaaagcta tgcgttgggg aggaaaaaat cttaaatttg caagacctat
     9901 aagatggata gttgcattgt taaatgataa agtattagag tttgatttag aaggaataat
     9961 atcttctaat gtaacaaaag gtcatagatt tttaggtgaa agtacaatag aagttgattc
    10021 tatagaagat tattttgaaa aattagaaaa aaattatata gttttagacc aaaacaagag
    10081 aaaagaaatg ataagaaaac aatgtataga agtagcaaat tctttaggtg gagaagttga
    10141 atttgatgaa gatttacttg aagaggtaac ttatttagtt gaatatccaa ctgcttttta
    10201 tggagaattt gatgtggatt atataaaact accaaaagaa gttgttataa ctcctatgaa
    10261 acaacatcaa agatattttc cagtacttaa agaaggaaaa ctgttaccta actttatagc
    10321 tgttagaaat ggtgattctt acagaataga caatgttaaa gctggaaatg aaaaagtttt
    10381 agaagcaaga cttgcagatg ctttattttt ctatagagaa gatactaaga gaaacttaga
    10441 aagctacata gaaaaattaa aaactgttgt attccaagta aaattaggaa ctatttacga
    10501 caaaacttta agacttgaaa aattaagtgc tgatatactt gatagtttag gtttagtaga
    10561 tgaaaaggca gatactataa gggctgctaa attatctaag gcagatttag ttactggaat
    10621 ggtaggagaa tttgatgaat tacaaggttt tatgggaaaa gaatacgcaa aagttggagg
    10681 agaaaatgat gttgtaagtg aagctatttt tgaacattat cttccaagat ttgcaggaga
    10741 tatacttcct aagactaatg caggggttgc cttatcaata gctgataagt tggattcaat
    10801 agcaggtttc tttgctatag gaatacaacc aacaggttct caagacccat atgctttaag
    10861 aagacaagct ttaggtatac ttagtatact tatggacaga aaagttgctg tggatataaa
    10921 aatacttatt gaacatgctt tagacaatta ttctgaatta gattttaata aagaagaagt
    10981 tgttgagcaa ataatgaatt tcttcaatga gagagtaaaa aatctattta aagatttagg
    11041 aataagatat gatgttgttg atgctgtttt aagttctgat ataaatgatg tttctgatat
    11101 gcatatgaga gctttagagc ttaataattg gctagaaaaa gatgaattag tggagatgct
    11161 tactgcattt aatagagttt ctactctagc tcaaaaagca gaaagtgata aaattgatga
    11221 atctttatta aaagaagatg ctgaaattaa actttacaat gaatttaaac aagtaaaggt
    11281 aaaagttaat gaattattaa aggataagaa atatggtatt gctttagatg aatttgcatc
    11341 tttaagacca gtagttgata atttatttga tacagttatg gtcatggata atgatgaagc
    11401 tattaagaac aatagacttg ctttacttaa acaagtatat gatactatgc taggaatttg
    11461 tgatttatct aaaattgttt ataaataaaa tttaaactat ttataagaaa taaggctgtc
    11521 tcaaaataga gatagtctta tttttcattg ttgagatgat gaaaatatat ttgatttcat
    11581 agaaaaagag tgtctcataa actaaaaagt tcattttgag acacccatgt tttttgtaag
    11641 taaatataat aaattatatt ttatacttat cagttatttt gtttaaaata ttatcatttg
    11701 aatttaccca ttcataaaaa ttgcctgaag atgcgtccga tattttataa agcatttcag
    11761 gaatatcact agctttaaaa tctccaagtg attttcccaa tctagtctta cctatttcaa
    11821 aactgcctcc aacaaataat tcaaatgcat ctgtagatat accatcaaca ttcttctttt
    11881 taccacataa acctatgcta ccaacttgat gaacaccaca tgaatttaaa catcctgata
    11941 tatataactt aggtgcttta tttaaaattt cttcattatt ttgtaatcta aaataatcta
    12001 tgatttcatg taacattttc tgactatttt gtatgcccat ttgacaaatt ggaacaccta
    12061 tgcaagatat actttgttct aattgagaat tacaactaat agttttactt atttctaata
    12121 ttctttttgc ttcatttcca tttaaattta aaatatataa tccctctgtc attcctagtc
    12181 taatcatagg attttcaaca ttatctaatt ctttaagtaa agtacttaaa tcctttgtta
    12241 acaaaatacc tccaactgga tgtatatata cagtgtataa accatcctgt ttttgaggta
    12301 ttagtctaga atcttgtata tctattttaa ctccaggttt agagtaatct atttcttcaa
    12361 tattgagttc aagactacca ttttcttttt ctaatttata gtattcttta aatttttcta
    12421 agaatagttc ttcaccaagt gattcgacca tatatctaac cctagctctg tttttatttt
    12481 cataatttcc ataatccata aagaatttaa tcataccttc aacacaatat aaggcgtctt
    12541 tcggctttat aagttcatca agttctaaag caactttggg ttgtttacct agacctccac
    12601 ctaagaaaac tctgaaatat ggttcatcat ttttcaaagt agcaacaaat cctaagtctt
    12661 gtatactaca atgagcagta tctgtgtaac aattagaata agaaactttt aattttctag
    12721 gtaaatggta tgtagtagca tttttgataa aatatttatc agtagcaact gcatatggag
    12781 taacatcaaa gacttcacta gggtcaactc cagataatgg agataatccg acatttctag
    12841 gataattacc accaccacct ctagtgaata tatcattttt tattccttct tccatgatat
    12901 caacaattga atttatagat aaatcgtgta attgtatagc ttgtctagtt gttaagtgta
    12961 tcttatctaa gttgtgttta atagctattt catagatttt attaagttga tactgagata
    13021 aaactccagc agatactcta agtcttatca taaaagattt tttgtctctt tgagcataaa
    13081 caccatatcc accagaaaat cccttatatt gcattttatt tatttctcca tttaaaaatt
    13141 ttgaagaata ttctttaaaa ttaggtagtt catttaacaa tatcttttta aaattatcca
    13201 ttatttcacc tcattgttag tcttgtataa aaatatatct attgtataat agatatatat
    13261 attattatct agtcaggata aaaatataac agaataattc tattaaaagt aagaataata
    13321 aggacatagt gagtattttg tttttatttt acaaattaag tataatattt ttgttttata
    13381 aaatataatt aagataaata gataattttg taattttaat atataatatt tagtagagtg
    13441 tgggagtagc acttaatatt tgcaggagga gtttttgtat atgggagaaa taataaatgc
    13501 attagataga gccctagaca taattttgct gttatatcat gaaaagagag aaatgggaat
    13561 aactgaaata agtaaggcta tgggagtata taaaagtact gtacatagaa ctttggttac
    13621 tcttgaaaac aaaggttttg ttatacaaaa tgcagagaat ggtaagtatt ggctaggaat
    13681 aaatttatat gcaataggta tggttgtggg agaaaaaatg tcattgactg aaatagtaaa
    13741 accatatacg aaaaaattaa atcaagaatt taacgaagta gttaatgtat ctatattaga
    13801 agaaagagct caagattctc caagaagtat cataatacat aaagagtatg gttcaaatca
    13861 attactttca gtaaatccat ccgttggttc aagtagtgaa tgttattgtt cagcagttgg
    13921 taaatgtctt atggcattta atgattctat agattttgaa aaatatagaa aaacaccaat
    13981 acacaagtat acagaacata caattgataa ttgggatgat atgatgcttt ttttagcaaa
    14041 aataaaagaa caaggatatg ctatagatga tgaagaacta gaacatggac taacttgtat
    14101 tggagcacct atcttagata aaaataataa agctattgca gccataagtt tgtcaggtcc
    14161 aactataaga atgagagaag gggactttga gtataagatt aaaagagtta tagaaactgc
    14221 aaaaagtata tctgaacttt ttagatgatt tttaatctcg ataataaaaa taggctatat
    14281 ttaaattgtt atttgtaaat atcagtttaa atgtagccta tttttcttta atctaaaatt
    14341 aaatattctt aaaatttaaa atgttattga aatatataag cgaatgtgtt atcttttaat
    14401 taagcaataa aaatagaaca aggttctata ctatagaaca aaattgatgc atatatagta
    14461 tttatacaaa atataaatat tatatattta aaattaagta aaaatattac ataaagggga
    14521 gatttgagaa atggaaaaag tcaagttgag tgttgaaaga tgtaagggat gctatctttg
    14581 tattgaagct tgcaaaaaag atgcaataag tctttctgac tacacaaata aaaaaggctt
    14641 tgtaactatt aaagtcgatg aagaaaaatg tattcaatgt ggcgcttgtt atacaatgtg
    14701 ccctgattta gtatttgaga tattataaat tatttttatt agaggggaga aaaggtatgc
    14761 aaagtttatt aattaaagga aatgaagctt tagcagaagg tgctatacgt ggtggttgca
    14821 gattttatgc aggttatcca ataacgccac aatcagaaat attagaaatg atggcatgga
    14881 gacaaaaaga agttggagga gtatttatcc aaggtgaatc agaaatagcc agtgtaaata
    14941 tggttatggg agcaagtgca cttggagcaa gagctatgac ttcttcatct ggtccaggat
    15001 tttcattaaa acaagaaggt attgcatatt gggtatcagc tggtttgcca gcagttgtag
    15061 catgtgtgca aagatatggt ataggtgatg gatttatagg agctggtcaa gataattatt
    15121 ggcaagcagt aaaaggcggt ggaaatggag attatcattt aatcgtgtta gcacctaata
    15181 gtgttcaaga aaatatggat atggcatatg aatcttttga attggctgaa aaatatatgc
    15241 atccagtact tatactttct gatgctacaa taggtcaaat ggtagaacct tgtatagcac
    15301 caccaatgaa agaatacgat atggataaag ctccatgggc tgcaaaagga acacctgtag
    15361 gagaaaaaaa taaagtaata actgatataa catattttga agaccaagat acatggcaaa
    15421 aagggtatat agaaaaaatg agaaaagttc aagaagatga acaaagatgg gaagagatag
    15481 aaatagaaga tgctgaagtg gtatttgtgg catacggtat ttcatcaaga atagcagaag
    15541 gtgcagttag agatgctaga gcaaaaggaa tgaaattagg tctaataaga ccaaaaacat
    15601 tgtggcctta cccaagaaaa gcatttaaga aattaagtga ttcattgaag ggacttgtaa
    15661 cagttgaaat gaacatgatg gcacagatga aggaagatgt aattatagca tgtgataata
    15721 gatttccagt atattcagta gcaacagctc aatatatggc agaagtaaat caattaatag
    15781 attttgctca acagattata gatggaaaag ctaagcaaga ggaggtattt taattatggc
    15841 taaaaaagca ccagcaataa tgatgtgtga aaatatgttc tgtggaggct gtggacactc
    15901 tatagtcgat agaattattg gagaagtatt aacagaaatg gatatatgtc aagatacaat
    15961 tatatgttca gatataggtt gtaatcatat gtttcaattt agtatgaatg tagatgttgt
    16021 cgttccacct catggtaaaa tgggagcagc aatggcagct atgaaaagag ttagaccaga
    16081 taaatatata tttacaatag ctggagatgg tggagcttat gcaatagggc ttggagaaac
    16141 tatgtcagct gctactagaa atgataatgt aatatttctt gttatgaaca ataatatgtt
    16201 tgcaatgaca ggagggcaaa tggctccaac tactatagaa ggtcaatata cagcttctac
    16261 tccagcgggt agaaattacg cagatcatgg ttcaacattt gatgttgtaa aagttatggg
    16321 aaatttagat atagcatatc tagcaagagg aacaataact aaccctaaag aaataaatac
    16381 aacaaaaaga tacatcaaaa aagctctaga aaaacaaaaa gaaaagaaag gtttttgctt
    16441 aatagaagtg cttgtaccat gtccaacaaa ctggggatta aagccagtag atgctatgaa
    16501 gagaattgaa aatgaacttg ttccaaagta tccacttgga gaatttattg attagggagg
    16561 aaataatatg ttgaatgaaa ttatatgtgc tggttttgga ggacagggag ttcttacagc
    16621 aggaaaaatt ataatgtatg ttgcatataa ggcagaaaag aaaattacat ggttcccatc
    16681 ttatggaaat gagatgaggg gaggaagtgc aaattgcaat gttgttgtaa gtgatgaaaa
    16741 aattgcaaat ccttgtgtaa aaaatccaga tatactattg ggaatgaaca gcgtatcagt
    16801 agataagtat gaaagtttaa tgaaaaaaaa tggttatatg tttgtcaatg aatcactagt
    16861 aagtaaggat agaaagtaca gagaagacat taatgttata aaagtacctg ttactgaaat
    16921 agcacaaaat ttaggcaatg aaaaagctgc taacatttgc atgttaggtg ctatggtaaa
    16981 gagtttggat ttatttacaa aagaagaatt tgaaagtggt atgtgtgaat actttgaaaa
    17041 tcaaggcaaa ggtaaattca attctaaaaa tgtagaagca tttgaagctg gatacgacta
    17101 cgaatgaata ttttaaatat ttaaaataat tattgaaata cttattttta tatgttagta
    17161 tttaattaat aaaatataga acgtcgttct ggacaatagg acgaagtttt taggtcggaa
    17221 tacaagaacg gcgttctgta tagtagaact agggggtgtt aaattgaaag ttttagtaat
    17281 aaatcctggt ggtacatcca caaaaatttc tgttttccaa gatgaaaatg aaattttgaa
    17341 aaaaaatata acccatacaa gagaagattt aaaaaatttt tctaaagtat ttgatgaata
    17401 tgattataga aaacaactga tagtagatat tttaagtagt gagaatcata gtataaattc
    17461 ttttgacgca gtagttggtc gtggtggact tatgaaggct ataaaaggtg gtacctatac
    17521 tgtgagtgaa gaaatgattg aggatatgag aaatgaaata aatggggaac atgcatcaaa
    17581 tttaggtgca ttattagcta aaactatagc tgatgaaata ggagtacaat catttgtagt
    17641 agacccagtt tctgtagatg aatttgatga tgtatcgagg attacaggaa tatcagacat
    17701 agaaaaagca agttggttac atgcattaaa ccataaagct gtttcaagaa aaattgcaca
    17761 aaaactaggg ggaaaatacg aggattataa ctttattgta gctcatttag gttcaggaat
    17821 atcgattgta gcacataaac aaggcaaaat gattgatgga agtggtggta ggtctgatgg
    17881 tccattttct ccagaaagga gtggtggatt acttgcatat cctcttatag aattgtgtta
    17941 ctccggaaaa tatactcatg atgaaatggt cgcaaaagta agttcgattg ggggaatgta
    18001 cgattattta ggaacaaagg acatgataga aattgaaaac agaatcaagg agggtgatga
    18061 aaaggcaaaa cttataatgg atgcatttat ttatcaagta gcaaaagaaa tttctatgta
    18121 tggagcttca ctttcaggga atgtagatag agttatcttg acaggaggga tatctcactc
    18181 agaaatggta gtcaaaggag tagcagaaaa agtttcatat ctagctccaa tagagatagt
    18241 accaggagag atggaaatgg aggcacttgc tctaggggca cttagagtac ttaatggaga
    18301 agaaatagca aaaacttatt aatatttagg agaaagagta tgttaaaaga ttttaataat
    18361 ttattagaag aggctaaaaa gcttgaacct gttattgtaa gtgttgctgt tgcagaagac
    18421 aaagaagtat taaaggctgt gaaatcagca acagatttaa acattataaa gccaattctt
    18481 gtaggagaca aggataaaat agaaagtata tcaagagaaa ttggattaaa aggtcatgaa
    18541 ataataggtt gtcaaaatcc cgatgaatca gttaaaaaag ctgtagagat agttaaaaat
    18601 aaggaagcta aagttcttat gaagggatta gtaaatacaa gtgtctatat gagagctata
    18661 ttaaataaag aaacgggtct tagaacagga agattattaa gtttgcttgc agtatatgaa
    18721 ttacctcaat atcataaact tctttattgt actgatagtg gaataaatgt atcgccaaac
    18781 ttaaatcaaa aaaaagatat aatgacaaat gcacttctta ctatgaaaag tataggcatg
    18841 gaaaatccta aaactgcgat acttactgca aatgaaatgg tagaccctaa gatacaatct
    18901 acagtggatg ccaacctact tgtagagatg gaaaaatcgg gagaaatacc aaagtgtata
    18961 gtggaaggtc caatagcatt tgatgtagca tttgatgcac atgcagcaga acataaaggc
    19021 attaaaagta atattgctgg tgatgttgat ttattagtat ttccaaatat agaagcaggg
    19081 aatatattag gaaagtcatg gcttagattt aataaagcta agtgggcagg tatagtttta
    19141 ggagcaacaa atccgataat tttaggttca cgttctgaca cagctgaaat aaaaataaat
    19201 tcaatagcat tagcgtgcct agcatcacaa taagaaatta ttattaagga ggaaaaaata
    19261 ttatgaaata tgcaaaaaga atggatatgg taaaagcttc agctataaga gattctcaaa
    19321 agaaaatagc tcagaaggta gcaagtggtg gaactgttat atcttttgct gcagggcttc
    19381 ctgacccaaa tttatttcca atagaagaga taagtgaaat aactcaaaaa gtacttaatg
    19441 aaaggggaaa atttgcccta gcatatggac ctactaaagg tgaagatgag ttgttagatc
    19501 tattagtaaa aagaatgaaa gaagaagaaa atattgatac aaaagcagaa aatattataa
    19561 taactactgg gtcacaacaa gggatagcat taagtgctat gattttatta gaaaagggag
    19621 atgtagtagt tacagaaaat ccaagttact taggtgcaat aaatgcattt agaccttatg
    19681 aatgtgattt tttaggagta gatacagatg aagaaggaat ggtaacagat gatttagaca
    19741 aattattatc tgaaaattct aacataaagg tgatatatgt aataccaaca tttcaaaacc
    19801 ctacaggaaa aacatggtct ttacaaagaa gaaaagattt tatggaagtc gtgaataaat
    19861 atgatgtcat agtaatagag gataatcctt atggagaaat aagatttaca ccagaacctc
    19921 tacctacact taaatctctt gatactaaag ataaagtatt gtaccttggg tcattttcaa
    19981 aggtattatg tccaggattt agagttgctt ggatgtgtgg taatgtagac atcatagata
    20041 aggcagaact tttaaaacaa ggtatagatt tacagtcaaa tcaattttcg caagtacaag
    20101 taatagaatt tttaaagaaa tacaacttaa atgaacatat agaaaaaata agagcagagt
    20161 ataagaaaag atgtctgcta atgttagatg ctatgaagaa acatttccca gaggaagcta
    20221 aatatacaga accagatgga ggaatgttta tatgggttga acttcctgaa agtataaatg
    20281 tagatgattt attagataaa gccattgatg ctggagttgc ttatgtttct ggagagtcct
    20341 tctttgcaaa taatggtcca aaaaatacta tgagacttaa ctatacaaca atgtcagaag
    20401 aacaaatcgt taaaggtgtt gagatacttg cagaagttat aaaaaaagaa ctcaattatg
    20461 cttgtgaata aatataaaaa gtgagggttt aaatatggga gaaaaagaaa acggtataaa
    20521 gtttggaaag ttaatgtttc aacttgttat tatagcaata tcttgggaat tagtttatat
    20581 tataccattt atccaatata cattatatga tccaatccta aaagcattag aatgtacaaa
    20641 cactcaacta ggttttctac ttacaattta tgggttaggt aatatatttg gagctcctat
    20701 aggaggctgg ttagcagata gatttgatta tagaaagata attttaggtt ctgtatttct
    20761 taatggagta gtatcatttt tatttgcatt taatatgaac tatccatttg cagttgctac
    20821 ttgggtaggt tgtgcaatca catcattagt tatgaactat ccttctatgg taaaaatact
    20881 tagagttata ggaaaagata atcaaggtaa ggtttatgga tttaatgaag ctatggttgg
    20941 agtttctgga gtagtaatgg gagcaatatt cttatatatt tatacatgct ttgcaactcc
    21001 tacattgggt atgagatggg taatgatatc tttaggaata ttatcaatag caatgtgtcc
    21061 agtattatgg tttgtaatta aagatgtaga tattaaagaa gaaagagaag aagcaggtga
    21121 aaaaatgtct gctggtgatt ttataacagt actaaaatca ccaaatactt ggctagtagg
    21181 aataagtata ttctgtgtat attcattcac agttacaatg tcttatttta caccatatat
    21241 aacatctgta cttggaggtt ctgtagcatt atctggtgca ttagcaataa taagacagca
    21301 tggtctaaaa ttatttggag caccatttgg aggatattgt gcagacaaat taaaatctcc
    21361 tacaaaagta cttttaccaa tatatgtatt tggtatagca gtaatagttt tattcctagt
    21421 attacctgca tcaactccaa tgactatatt catagcttta acatttgtag taggtatatt
    21481 aggttacatg ggaaaaggta tatattatgc agtacaagat gaagtaaaag ttccagttaa
    21541 atattcagca actactatag gtatagctgc agctttagga ttttcaccag atgtattcca
    21601 atttgcatta ataggacact ggatagatac ttatggcaat aaaggttaca catatacatt
    21661 tatattccaa atagcaattc tagttattgg aatactatct tgtttatata tattaaaggt
    21721 taaaaagaga agagcaagta aattagaagc ttaaatattc aatttggtaa ttaagtatat
    21781 ataaatacaa tacttatttt gatgaatgtg attgtataaa taacataaaa aatatcaata
    21841 gattaagttt tataataatg tttagagaat aaagtataaa aatcaaaatg ttatcaagtt
    21901 ttctaggttt taacataaag ttacccctga aggtgtaaaa ttgagtggca attattatct
    21961 attttcttac ctacataaac ctatttaatt taaattctaa gagattttca aaatagatgg
    22021 tagttgtact caattttaaa cctcataata aatacgaaaa tttaaaatct aactagatac
    22081 tatttaatta tatattaata aatatatatt gtactattta tataataata atttcaggct
    22141 tttcattata tgtattccaa tttatattgt ttgattatta ttcaaacaca cgtcgttaaa
    22201 aattttataa atttatgttt agat
//
LOCUS       DAGSWI010000062        21284 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.062, whole genome shotgun sequence.
ACCESSION   DAGSWI010000062 DAGSWI010000000
VERSION     DAGSWI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21284
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.062"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..537
                     /gene="tuf"
                     /locus_tag="MJF80_002144"
     CDS             <1..537
                     /gene="tuf"
                     /locus_tag="MJF80_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBZ0280804.1"
                     /translation="VFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVE
                     MFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGG
                     RHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFS
                     IREGGRTVASGVVATIIE"
     gene            909..1220
                     /gene="rpsJ"
                     /locus_tag="MJF80_002145"
     CDS             909..1220
                     /gene="rpsJ"
                     /locus_tag="MJF80_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBZ0280805.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            1312..1941
                     /gene="rplC"
                     /locus_tag="MJF80_002146"
     CDS             1312..1941
                     /gene="rplC"
                     /locus_tag="MJF80_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBZ0280806.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1971..2594
                     /gene="rplD"
                     /locus_tag="MJF80_002147"
     CDS             1971..2594
                     /gene="rplD"
                     /locus_tag="MJF80_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBZ0280807.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2594..2884
                     /gene="rplW"
                     /locus_tag="MJF80_002148"
     CDS             2594..2884
                     /gene="rplW"
                     /locus_tag="MJF80_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBZ0280808.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2914..3744
                     /gene="rplB"
                     /locus_tag="MJF80_002149"
     CDS             2914..3744
                     /gene="rplB"
                     /locus_tag="MJF80_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBZ0280809.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3779..4060
                     /gene="rpsS"
                     /locus_tag="MJF80_002150"
     CDS             3779..4060
                     /gene="rpsS"
                     /locus_tag="MJF80_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBZ0280810.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            4092..4427
                     /gene="rplV"
                     /locus_tag="MJF80_002151"
     CDS             4092..4427
                     /gene="rplV"
                     /locus_tag="MJF80_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBZ0280811.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            4450..5265
                     /gene="rpsC"
                     /locus_tag="MJF80_002152"
     CDS             4450..5265
                     /gene="rpsC"
                     /locus_tag="MJF80_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBZ0280812.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            5301..5732
                     /gene="rplP"
                     /locus_tag="MJF80_002153"
     CDS             5301..5732
                     /gene="rplP"
                     /locus_tag="MJF80_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBZ0280813.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5734..5937
                     /gene="rpmC"
                     /locus_tag="MJF80_002154"
     CDS             5734..5937
                     /gene="rpmC"
                     /locus_tag="MJF80_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBZ0280814.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5961..6215
                     /gene="rpsQ"
                     /locus_tag="MJF80_002155"
     CDS             5961..6215
                     /gene="rpsQ"
                     /locus_tag="MJF80_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBZ0280815.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            6241..6609
                     /gene="rplN"
                     /locus_tag="MJF80_002156"
     CDS             6241..6609
                     /gene="rplN"
                     /locus_tag="MJF80_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBZ0280816.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6633..6938
                     /gene="rplX"
                     /locus_tag="MJF80_002157"
     CDS             6633..6938
                     /gene="rplX"
                     /locus_tag="MJF80_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBZ0280817.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6968..7510
                     /gene="rplE"
                     /locus_tag="MJF80_002158"
     CDS             6968..7510
                     /gene="rplE"
                     /locus_tag="MJF80_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBZ0280818.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            7528..7713
                     /locus_tag="MJF80_002159"
     CDS             7528..7713
                     /locus_tag="MJF80_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBZ0280819.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7746..8144
                     /gene="rpsH"
                     /locus_tag="MJF80_002160"
     CDS             7746..8144
                     /gene="rpsH"
                     /locus_tag="MJF80_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBZ0280820.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            8175..8717
                     /gene="rplF"
                     /locus_tag="MJF80_002161"
     CDS             8175..8717
                     /gene="rplF"
                     /locus_tag="MJF80_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBZ0280821.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8753..9121
                     /gene="rplR"
                     /locus_tag="MJF80_002162"
     CDS             8753..9121
                     /gene="rplR"
                     /locus_tag="MJF80_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBZ0280822.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            9142..9651
                     /gene="rpsE"
                     /locus_tag="MJF80_002163"
     CDS             9142..9651
                     /gene="rpsE"
                     /locus_tag="MJF80_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBZ0280823.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9666..9851
                     /gene="rpmD"
                     /locus_tag="MJF80_002164"
     CDS             9666..9851
                     /gene="rpmD"
                     /locus_tag="MJF80_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBZ0280824.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9884..10327
                     /gene="rplO"
                     /locus_tag="MJF80_002165"
     CDS             9884..10327
                     /gene="rplO"
                     /locus_tag="MJF80_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBZ0280825.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            10371..11639
                     /gene="secY"
                     /locus_tag="MJF80_002166"
     CDS             10371..11639
                     /gene="secY"
                     /locus_tag="MJF80_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBZ0280826.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSDEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11656..12306
                     /locus_tag="MJF80_002167"
     CDS             11656..12306
                     /locus_tag="MJF80_002167"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBZ0280827.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            12307..13053
                     /gene="map"
                     /locus_tag="MJF80_002168"
     CDS             12307..13053
                     /gene="map"
                     /locus_tag="MJF80_002168"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBZ0280828.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            13075..13344
                     /locus_tag="MJF80_002169"
     CDS             13075..13344
                     /locus_tag="MJF80_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBZ0280829.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            13382..13600
                     /gene="infA"
                     /locus_tag="MJF80_002170"
     CDS             13382..13600
                     /gene="infA"
                     /locus_tag="MJF80_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBZ0280830.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13636..13749
                     /gene="rpmJ"
                     /locus_tag="MJF80_002171"
     CDS             13636..13749
                     /gene="rpmJ"
                     /locus_tag="MJF80_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBZ0280831.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13902..14273
                     /gene="rpsM"
                     /locus_tag="MJF80_002172"
     CDS             13902..14273
                     /gene="rpsM"
                     /locus_tag="MJF80_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBZ0280832.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            14309..14707
                     /gene="rpsK"
                     /locus_tag="MJF80_002173"
     CDS             14309..14707
                     /gene="rpsK"
                     /locus_tag="MJF80_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBZ0280833.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14739..15362
                     /gene="rpsD"
                     /locus_tag="MJF80_002174"
     CDS             14739..15362
                     /gene="rpsD"
                     /locus_tag="MJF80_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBZ0280834.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            15440..16387
                     /locus_tag="MJF80_002175"
     CDS             15440..16387
                     /locus_tag="MJF80_002175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBZ0280835.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            16408..16749
                     /gene="rplQ"
                     /locus_tag="MJF80_002176"
     CDS             16408..16749
                     /gene="rplQ"
                     /locus_tag="MJF80_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBZ0280836.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16869..17702
                     /locus_tag="MJF80_002177"
     CDS             16869..17702
                     /locus_tag="MJF80_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBZ0280837.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17690..18556
                     /locus_tag="MJF80_002178"
     CDS             17690..18556
                     /locus_tag="MJF80_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBZ0280838.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18550..19353
                     /locus_tag="MJF80_002179"
     CDS             18550..19353
                     /locus_tag="MJF80_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBZ0280839.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            19385..20116
                     /gene="truA"
                     /locus_tag="MJF80_002180"
     CDS             19385..20116
                     /gene="truA"
                     /locus_tag="MJF80_002180"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBZ0280840.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            20236..20667
                     /gene="rplM"
                     /locus_tag="MJF80_002181"
     CDS             20236..20667
                     /gene="rplM"
                     /locus_tag="MJF80_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBZ0280841.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20696..21088
                     /gene="rpsI"
                     /locus_tag="MJF80_002182"
     CDS             20696..21088
                     /gene="rpsI"
                     /locus_tag="MJF80_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBZ0280842.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8518 a         2515 c         4454 g         5797 t
ORIGIN      
        1 gtattctcaa tcacaggaag aggaacagtt gcaacaggaa gagtggaaag aggagtacta
       61 aaagtacaag acgaagtaga attagtagga ttaacagaag caccaagaaa agtagtagta
      121 acaggagtag agatgttcag aaaattatta gaccaagcac aagcagggga taatatagga
      181 gcattattaa gaggagtaca aagaaacgag atagaaagag gacaagtact agcaaagact
      241 ggatcagtaa aggcacacac aaagtttaca gcagaagtat atgtacttaa aaaagaagag
      301 ggtggaagac atacaccatt ctttgatgga tatagaccac aattctattt cagaacaaca
      361 gacgtaacag gagcttgtaa gttaccagaa ggaatagaga tggtaatgcc tggagataac
      421 gtaacaatgg aagtagactt aataaactca atagttgtag aagagggatt aagattctca
      481 ataagagaag gtggaagaac agtagcttca ggagttgttg ctacaataat agagtaatcc
      541 tttactaaac taatggattt aatataaaaa aagagggggg taactcctct cttttttgtt
      601 ttttgttgac aagtagcgct aaagcatata tactaaacta gtgccttgag acagattaac
      661 aatcaagttt aaggttatga tgttgtaata gaaaataaaa ttgaaaaaaa cagaaaaaaa
      721 acttgaaaaa atgtaacaga tgatataaaa tataatagtg tgcttgagag attaagaaaa
      781 taataattaa atcctagtga aatacgaact gatatcgatt taaaagttac aacacacccg
      841 gagcagtgta tcggtattgg ttgtacgcaa cttgaaaatt aaacacgtga taaaggaggg
      901 aaaacaaaat ggctaagaat gaaaaaataa gaataagatt aaaatcatat gatcacaaat
      961 tattagattt ttcagcaggt aaaatagttg aaactgctaa aaaggctgga tcacaagttt
     1021 caggacctgt gcctctacca acagaaaaac aagttgtaac tatattaaga gctgtacata
     1081 agtacaaaga ctctagagaa caatttgaaa taagaactca taaaagacta atcgacatag
     1141 ctaatccaac acctaagaca gttgactcat taatgagatt agacttacca gcaggtgtag
     1201 atatagaaat aaaattataa aaacccctaa gatattatcc tatagatgat tatcttagaa
     1261 tgattactct aaaagatgta atccgctgta agaattatag gaggtgctaa tatgaaagga
     1321 atattaggca aaaaagttgg tatgactcaa atattcactg ataaaggtgt agttatacct
     1381 gtaacagctg ttgaagcagg gcctatggtc gttactcaaa taaaaacagt tgataaagat
     1441 ggatacaatg ctatacaaat aggttttgaa gatgctaaag aaaaagcttt aaacaaacct
     1501 aaaaaaggac atttagctgc tgctaatgtt ttaaagaaac atttaaaaga atttagagta
     1561 gattctgtag aaggatacac agtaggacaa gaaataaaag ctgatgtatt tgaagcaggt
     1621 gcaaaaatag atgttactgg aataagtaaa ggtaagggat tccaaggtcc aataaaaaga
     1681 catggacaaa gtagaggtcc tgaaactcac ggttctagat accacagaag accaggttca
     1741 atgggagctt gttcttaccc aggtagagta tttaaaaaca aaaaattagc tggacatatg
     1801 ggaagcgtaa aggtaacagt tcaaaactta gaggttgtta aagtagatgc tgacaagaac
     1861 cttatattag ttaaaggagc tataccagga gctaaaggtt cagtagtaac tataaaagaa
     1921 gctataaagg tttctaaata atgactaacc tagagaaagg aggaataaca atgccaaaat
     1981 taaatgtact aaatgttagt ggacaaaacg ttggagaaat agaattatca gattctatat
     2041 ttggcgtaga agttaatgga catgttttat acgaagttgt aaaaaatcaa ttagcaaata
     2101 agagacaagg aactcaatct gctaaaacta gagcagaagt tagaggtggc ggaagaaaac
     2161 cttggaaaca aaaaggaaca ggtagagcaa gacaaggttc tacaagatct gtacaatggg
     2221 taggtggagg agttgctttt gcacctaaac caagaagcta caaatataca ttacctaaga
     2281 aggtaagaag attagctatg aagagtgctt tatcttcaaa agttcaaaat agtgaagtta
     2341 tagtattaga tgcattaaac atggatgctc ctaagactaa agaatttgct caaatattaa
     2401 acaatataaa tgctgctaaa aaagctttag tagttatagc tgacaaaaac gataacgtaa
     2461 taaaatcagc tagaaatata gaaggtgttc aaactgcatt agttaatact atgaatgtat
     2521 atgatatatt aaaatacgat tcatttataa taactacaga tgctgttaaa aaagtggagg
     2581 aggtgtacgc ataatgacta atccacatga tgtaataata agaccagttg taactgagca
     2641 cagtatggct gaaatgggtg aaaaaaaata cacttttgtt gtggctaaag atgcgaacaa
     2701 aactgaaata aaaaaagcag tagaaaaagt attcggagtt agtgttgata aagtaaatac
     2761 tttaaactac gatggaaaag ttaaaagaat gggtagaact caaggaagaa catcaagctt
     2821 taagaaagca gtagttaaat taactgctga tagtaaagaa atagaattct tccaaggaat
     2881 gtagtattat aacaaacgaa ggagggaaaa cagatggcta taaaaaagtt tagaccaact
     2941 tctcctgcct taagacaaat gacagtttta gtttctgatg aaataacatg taatcaacca
     3001 gaaaaatctc ttttagttaa cttaaagaaa aatgctggta gaaacgtaca tggtagaata
     3061 actgttcgtc atagaggtgg aggacaaaaa agaaaatata gaataataga ttttaaaaga
     3121 gataaagatg gtatacctgc taaggttgct actatagaat atgatccaaa cagaactgca
     3181 aatatcgcat tattaaacta tgctgatgga gaaaaaagat atatattagc tccagttgga
     3241 attaatgttg gagatactat attatcagga ttaggtgctg atataaaacc aggaaactgt
     3301 ttagcattaa aagatatgcc agttggtact atcattcata atatagaatt aaagccagga
     3361 aaaggagctc aattagttag atctgctgga gtttctgcac aattaatggc taaagaagga
     3421 aaaaatgctt tattaagatt accatcaggt gagatgagat tagtaagcat aaactgtaaa
     3481 gctactatag gtcaagttgg aaatatagag catggtaacg tagttatcgg taaagcaggt
     3541 agaaaaagac atatgggaat aagacctact gtaagaggtt ctgtaatgaa ccctaatgac
     3601 catccacacg gtggagggga aggtagatct ccaataggta gaccatctcc tgttactcca
     3661 tggggtaaac cagctcttgg atacaaaact agaaagaaaa ataaagcttc aaataaacta
     3721 atagtatcaa gaagaactaa gtagtaaatt tatttttgct cggaaaggag gaatataaat
     3781 gtcaagatca actaaaaaag ggccttttgt ccatgcaaga cttttaaaga agatagaagc
     3841 tatgaatgct agtggaaata aagaagttat aaaaacttgg tcaagatctt caactgtatt
     3901 tccacaaatg gtagaaaaca ctatagctgt tcatgatgga agaaaacatg ttccagtata
     3961 tataacagaa gatatggttg gacataaatt aggtgagttc gttcctacta gaactttcaa
     4021 aggacataag gatgacgaaa aatctaataa gagaaaataa ttaaattatg actaagaaag
     4081 gaggaataac aatggaagca aaagctactg ctaaatacgt acgtgtatca ccaagaaaag
     4141 caggccaaat atgtgacctt gttagaggaa agaatgttga tgaagcatta gcaatattaa
     4201 agtttactcc aagaggagca gcttcaataa tagctaaggt tgtaaaatca gctaaagcta
     4261 acgcagaaaa caatcacgaa atggatactg aaaaattata tatagcatca atagttgcta
     4321 atcaaggacc aacaatgaag agattcatgc ctagagctat gggtcgtgca actacaataa
     4381 gaaaaagaac ttctcatata gaggttgttg ttaaagaaaa aaaataatta atagatagga
     4441 gggaaagtaa tgggtcaaaa ggttaatcca cacggcttaa gggtcggtgt tataaaagat
     4501 tgggactcaa gatggttcgc tactgataaa aaagagttcg gaaacttatt attagaagac
     4561 cataatatac gtaaattctt aaagaaaaga ttatactcag ctggagttgc taagatagaa
     4621 atagaaagat cagctaacaa aataaaaatg gacttacacg ttgctaagcc tggtgtagtt
     4681 ataggaagag ctggtgcagg aatcgaagca ttaaaagctg aattagaaaa aatgacaaag
     4741 aaaactataa tagttaatat agtagaagta agaagtacag ataaaaatgc tcaattagta
     4801 gctgaaaata tagctttagc tatagagaga agggttgctt tcagaagagc aatgaaacaa
     4861 gctatacaaa gagcaatgaa atctggtgct aaagggataa aagtttctgc ttcaggaaga
     4921 ttaggtggag ctgaaatggc tagaactgaa ggttatagtg aaggaaatgt gccactacaa
     4981 actttaagag cagatataga ttatggtttc gctgaagctg atacaactta tggaaaaatc
     5041 ggtataaaag tttggatatg taatggtgaa gttttaccaa ctagagacgg tgtaaatcca
     5101 agagaagaaa gcagaaagag cgatagaaga gacaacaaga gagataatag aagaaacgat
     5161 agaagaggaa atgacagaag aggaaatgat aacagaggaa actacagagg acaaagacct
     5221 caaggtggat caagacctca aagaactgaa aacaaaggaa attaataata gttaggttca
     5281 aggaaggagg aaaaatactc atgttaatgc caaaaagagt aaagcgtcgt agagttcata
     5341 gaggaagcat ggctgggcaa gctcataagg gtaataaagt tacttatggt gagtttggat
     5401 tagtagcatt agaagcttct tggataactt ctaatcaaat agaagctgcc agaatcgcga
     5461 tgactagata tataaaaaga gggggaaagg tttggataaa aatattccct cataaaccag
     5521 taacaagaaa accagcagag actcgtatgg gtgcagggaa aggttctcca gaatattggg
     5581 tagctgtagt taagccagga agagttatgt ttgaattagc aggtgtttct gaagataaag
     5641 caagagaagc tatgagactt gctgcacata aattaccaat caaatgtaaa tttgttaaaa
     5701 aagaagattt agaagtaaag ggtggtgaat aggatgaaag ctaaagaatt aagagattta
     5761 acaagcgaag agctaatgaa caaattaaac gacttcaaaa gtgaattatt tagcttaaga
     5821 ttccaattag ctactggtca attagaaaat acagctagaa taaagtttgt taagaaggat
     5881 atagcaaaag ttaaaactgt tcttgctgaa agaaagttat acgaaactag agcttaattt
     5941 ggaaaggagg ctttttaaac atggaaagag gaagaagaaa agttagaata ggccgtgttg
     6001 ttagtgataa gatggataaa acaatagtag tagctgttga agaattcgta cgtcatccac
     6061 tatataataa acgtgttaag agaactaaga aattcaaggc tcatgatgaa aagaatatat
     6121 gtaacatcgg agatagagtt aaaataatgg aaactagacc attatctaaa gataagagat
     6181 tcagactagt tgaagttgtt gagaaagtta agtagttttt gaagaaggag ggattacgat
     6241 atgatacaac aagaatcacg tctaagagtt gctgataact caggagctaa agaactttta
     6301 tgtatacgtg ttctaggcgg aagtaaaaga agatatggta acataggcga cgttatagtt
     6361 gctactgtta aaagtgcaac acctggtgga gttgtaaaaa aaggtaaggt tgttaaagct
     6421 gttatagtaa gaagcaaaca aggcgtaaga cgtaatgacg gaagctatat atcttttgat
     6481 gaaaatgctg ctgttataat aaaggacgat aaaactccag taggaactcg tatattcggg
     6541 cctgttgcta gagagttaag agacaatgaa ttcatgaaaa tagtttctct tgctccagaa
     6601 gtactataat taaggaggtg caataggaca tgatgcgtgt taaaaaaggt gacactgttg
     6661 tagttatagc aggtaaagat aaaggtaaaa aaggttcagt tttaaaggta tatcctaaaa
     6721 ctagcaaagt gttagttgaa ggtgtaaatg taataactaa acatcaaaaa ccaagtgcta
     6781 tgaaccaaca aggtggaata ataaataaag aagccccaat acacatatct aatgtaatgc
     6841 catttgatcc tgaaacagga aaaggcgtta gagtaagata tgaagtaaaa gatgggaaca
     6901 aagtaagagt atcagcaaag agcggaaaag aattataata aatattgctt gaaaggaggg
     6961 accttaaatg gcttctagat tacaagaaaa atacatgaaa gaagttgctc ctgctttaat
     7021 ggagaaattt ggatacaaaa acgtaatgga gatacctaag ttaaataaaa tagttattaa
     7081 catgggtata ggtgacgcta gagaaaatcc aaaaggatta gaaaaaggcg ttgaagaatt
     7141 agaaatgata tcaggacaaa agcctgttat aactaaagct agaaaatctg ttgctaactt
     7201 caaattaaga gaaggaatgc caataggaac aaaagttaca ttaagagctg acaaaatgtt
     7261 ctacttcatg gacaaattag tttcagtttc tttaccaaga gttagagact ttagaggagt
     7321 taaccctaat gcttttgatg gtagaggaaa ttatgcttta ggagttaaag aacaattaat
     7381 attccctgaa atagaatatg ataaaataga taaagtaaga ggaatggata taatatttgt
     7441 tactacagca aaaactgacg aagaagctcg tgaattatta aaattattag gaatgccatt
     7501 ttctaagtaa gcaaaggagg gattcccgtg gctagaaaag cgatggttgt taagcaacaa
     7561 agaaagcaaa agtacgctac tagagaatac actagatgta ctatatgcgg aagaccgcat
     7621 tcagttttga aaaaatttgg tatatgccgt atatgcttta gagaattagc ttataaaggt
     7681 caaatacctg gtgttagaaa agcaagctgg tagaagcttg attagaatgg aaggagggca
     7741 acaaaatgac aatgacagat ccaattgcag atatgcttac tcgtataaga aatgctaatg
     7801 ttgttaagca tgaaactgta gatgttccag cttctaatat gaagaaagaa ttagctagaa
     7861 tcttattaga agaaggtttc ataagaggtt atgatgttat agaagatgga aaacaaggta
     7921 tcataagaat acaattaaag tacggacaag aaggcgaaag agttataaca ggtcttaaga
     7981 aaatatctaa gcctggaatg agagtttacg ctgctaacca tgagatacca aaagtattaa
     8041 acggattagg aatatcagtt atatctactt caaaaggaat attaactgac aagcaagcta
     8101 gaaaagaaaa tgttggtgga gaagtaatct gctacgtttg gtaattaata tgaacaatgt
     8161 aaggaggtgc gaccatgtca agaataggtg ttaagccaat aataatacca gcaggtgttg
     8221 aagttactat agctgaagga aatttagtta cagtaaaagg tccaaaagga actttaacta
     8281 aacaattaag tgctgagtta aacataaaaa aagaagaaaa tacaataatg gttgaaagac
     8341 caactgataa taaaaaacat agatctttac acggactaac tagaacttta ttagataata
     8401 tggttgtagg tgtaaatact ggtttcgaaa agaaattaga attaaaaggt gttggatata
     8461 gagctcaaaa acaaggaaag aaattagtta tgaacttagg tttctctcat ccagttgaga
     8521 tggaagatcc agaaggaata actgttgaag ctccaaacca aacagagtta atagtaaaag
     8581 gtatagacaa gcaattagta gggaattatg ctgctaagat aagagcttgg agaaagccag
     8641 agccatacaa aggtaaaggt ataaaatacg ttgatgaagt aatcagacgt aaagaaggaa
     8701 aaactggtaa aaaataatac caaaacttaa acgctagaaa ggagtgagct aggtgttaaa
     8761 aaaggctgat aaaaatgcta atagacttca aagacataag agagttcgta gaaaaataag
     8821 cggtacttct caaagaccaa gattatgtgt atttagaagt gctaacaata tatatgctca
     8881 aataatagat gacacaaaaa gagtaactct agttgctgca tcttctttag aagcagaagt
     8941 taagagtgct gtcaatcata caggaaacaa ggaagcagct aagaaagttg gagaattagt
     9001 tgctaaaaaa gctgttgaaa aaggaatcac tgaagttgta tttgacagag gtggatactt
     9061 atatcatgga agaattcaag aattagctga aggtgctaga gaagctggtc ttaagttcta
     9121 atacgaagga gggaaaaata catgctacgt cgtaagccga tagatgcagg acaacttgac
     9181 ttacaagaaa aagttgttga agtaagacgt gttactaagg ttgtaaaagg tggtagaaat
     9241 ttcagatttg cagcattagt agttgttgga gatgaaaacg gacacgttgg tataggtgct
     9301 gggaaagcaa tggaagtacc agacgctata aaaaaagcag ttgaagatgc taagaaaaat
     9361 ctaatagtag tgccaatagt tgggactaca attcctcacg aagtacgcgg acactttggt
     9421 gctggaaata tattaataat gcctgctgta gaaggtacag gagtaatagc tgggggacct
     9481 gctagagccg tacttgagct agctggacta aaagacgtta gagctaaatc tttagggtct
     9541 aacaatccaa gaaatatggt aaatgctaca atagaaggac ttaattctct aaaaacagtt
     9601 gaagatatag ctaaacttag aggtaaaaaa gttgaagaac ttctagggta aggaggtagt
     9661 gtaaaatggc taaattacaa ataaagttag ttagaagtgt tataggaact actcctaacc
     9721 aaaaaaagaa tgttgaagcg ttaggattaa gaaaaagaga acaagttgtt gtaaaagaag
     9781 acaatgctca aacaagaggt atgataaata aagtaagtca cttattagaa gtaactgaaa
     9841 tagctgaata atttttataa agtagtgcaa ggaggtgcaa tccatgaagt tacatgagtt
     9901 aaaacctgct gaaggtgcag taagagctaa gagaagatta ggtagaggta ctgcaactgg
     9961 acaaggtaaa actgcaggac gtggacaaaa aggtcaatgg tctcgttctg gtggtggagt
    10021 aagagtagga tttgaaggtg gacaaatgcc actagctaga agacttccta agagaggttt
    10081 caataacata tttaagaaag tttacacaga agttaatgtt gaagttttaa acagatttga
    10141 aaatggaaca gaaataactg cagaattatt aaaatctact aaaactataa gcaaaatagg
    10201 taaagacgga attaaaatct taggtgaagg taatttagag aaagctttaa ctgttaaagc
    10261 tgctaaattt acagcttcag ctcaagaaaa aatagaaaaa gctggtggaa aagcagagtt
    10321 agtataataa cctgctaatc tcctagccag tacaggcggg gtgaattagc gtgctgtcaa
    10381 aattaaaaca agcttggaag attaaagcag taagaaaaaa agtaatgtat acacttatga
    10441 tgatcgtgat attcagaata ggtactacta taccagttcc aggcatagac actagtatta
    10501 tacaaaagat ggtaggcggc aatagtctac tttctctgta taatatgttt actggtggag
    10561 catttagtaa cttttcacta tttgctttag gtataagtcc atatatcact gcatcaatca
    10621 taatacaact tctaacagta ggttttgaaa gtttagcaga acttcaaaaa tctggtgaag
    10681 aaggaaagaa aaaaatcaat aagtacacta aatatacagc actagctcta gcagttgtac
    10741 aagctttggg aattacatta ggaattgtaa gaagtgcttt aatatcaaat agtgtgtttt
    10801 ttatcacaac tgttgtaata acattagttt cagcaagtat gttagtaatg tggattgggg
    10861 acaaaattac tgaaaaagga atcggtaatg gtagttcagt aataatattt gcaggtataa
    10921 tatcaagaat acctacagat gttattaaaa tatcgcaaca agttaaatct gacgaagttg
    10981 caccttgggt tatagtgatt ctagctgtag ttatattact tacagtaaca ggagttacat
    11041 ttatacaaga agcaactaga aagatacctg tacaatatgc taaaagagtt gttggaagaa
    11101 aaatgtatgg aggacaaagt tctcatatac caatgaaagt taaccagtca ggagttatgc
    11161 ctataatttt tgctagttca cttttagctt tccctcaaac aatagctatg tttatgggtc
    11221 caaatgctca ggcttttgtt caaaaatact taagtatggc aacagaacaa ggattttgga
    11281 catatagatc aattgagatt ctacttataa ttttcttctc atatttttat acaactgtat
    11341 catttaatac agaagacata tctaaaaata tgaaaaataa tggtggattt ataccaggta
    11401 taagaccagg tgagcctact atggattatt taaatagaat tttaactaga ttaactttag
    11461 ctggagctac tttcttagca ataattgcta tggtgccagc tcttacaaca cattatatga
    11521 aagtaaatat gagtctagct gggacttcat tgctaatcgt tgttggagtt gctttggagc
    11581 ttaagagaca gttagagtca aacttagtta tgagaagcta tcaaggtttc ttaaaataaa
    11641 atggagatgg ataatatgag aataatatta cttggacctc caggtgctgg taaaggtact
    11701 caggcggcag gaatagtaga aaaatacaat atacctcata tatcaacagg agatatattc
    11761 agaaagaata taaaagaggg aacagaactt ggaaaaaaag ctaaagaata catggaccaa
    11821 ggtttattag taccagatga gttaactgta ggtttagtta ctgatagaat atctcaagaa
    11881 gattgtaaaa atggatttat gttagatgga tttccaagaa atgtagcaca aggagaacat
    11941 ttagatatct tcttaaaaaa tgctggtata tcactagata aagttgtcaa tattgaagtt
    12001 gataagagta tattagtgtc tagagcagtt ggtagaagaa tatgtaagtc ttgtggagct
    12061 acttaccatg ttgagtttaa tcctcctaaa gtagaaggtg tatgtgatgt atgccaagga
    12121 gaattatatc aaagagctga tgataatgaa gaaactgtat ctaagagaat acaagtttat
    12181 ttagatgaaa ctaagccttt agtagattat tatagcaaac aaggtataat agcagatata
    12241 aaaggtgatc aagcaataga taaagtattt gaagatattg tcgcagcctt aggaagtgga
    12301 aaataaatga ttatcttgaa atcaaaaaaa gaaatagaac ttttaagaga agcaggtaaa
    12361 atagttgctg acactcatga agttttaaga aaagctattt ctccgggaat atctacttta
    12421 gaattagata aaatagctga agagaatata agaaaataca atgcagaacc ttcttttaaa
    12481 ggatatggag gttttccagg aagtatatgt gcttctatca atagagaggt agtacatggt
    12541 attccaggag aaactatatt acaagaaggt gacattgtaa gtttggatat aggagcctat
    12601 tataaaggat atcatggtga ttctgcgaaa actcacggag taggcatgat atctgaagaa
    12661 gataggaaat tgatagaagt aactagagaa agcttctatg aaggaataaa atttgctaaa
    12721 ttaggatata gactttcaga tatctcacac gcagtacaaa cacacgttga gaaacatggt
    12781 ttctcggtag ttagagattt agtgggtcat ggagttggag caaatcttca tgaagaccca
    12841 caagtaccta attatggact cccaggaaaa ggaccaagac ttagagaagg aatggttatt
    12901 gctatagagc caatggttaa tgcaggtcgt tatcatgtaa agactctatc agatgggtgg
    12961 acgactgtta caatagatgg aaaaaaatca gctcattatg agcatacgat agcaattact
    13021 gaacatgaac ctttgatatt gacaaagttg taatttaaaa agtaggtgaa aaaagtgcta
    13081 tcagataatt taagtatagg tcaagttgtt aaagtttcat taggacgaga taaaggaaat
    13141 ttattctttg tagtcaaaat aattaataat gaatatgtat taatagctga tggaaaaaag
    13201 agaaaacttg acaaacctaa gttaaagaaa gtgaagcact taaaaaaata taattttatt
    13261 aatgatgaag ttagaaaaag agtagtatct ggacaagaga ttacagattc ttttttaaga
    13321 gctgaactta ctaagttaaa ttagataatt taacttattg aaatggaggg ctggttagtt
    13381 aatggccaaa aaagatgtta tagaattaga aggtacagtt tcagaagctt tacctaatgc
    13441 tatgttcaaa gttaaactag aaaatggaca tgagatatta tgccacattt ctggaaagct
    13501 aagaatgaac ttcataagaa ttcttgaagg tgacaaggtg aatgttgaac tttctccata
    13561 tgaccttaca agaggaagaa ttacttggcg taagaagtag actatattaa tatttagtct
    13621 aaggagggat taataatgaa ggttagacca tcagtaaaac caatatgtga aaaatgtaaa
    13681 gtaataaaaa gaaaaggaaa agtaatggtt atctgtgaaa atccaaagca taagcaaaag
    13741 caaggataag aaattattac tagcattttg ttaaatttta tagtataata ctatattgtg
    13801 atgttaacaa aagaacgtta aaatcgttga acaatctggc gtaggattgg catatgatgt
    13861 gaagttttaa acactaaaaa ctatgtgtag gaggtgcgat catggcaaga atagctgggg
    13921 tagatttacc tagagaaaaa agagcggaga taggcttaac ttatatatat ggtataggta
    13981 aagcaactgc taacgaaata ttagctaagg ctgagataaa tccagatact agaataaaag
    14041 atttatcaga agatcaagtt aatgagttaa gaaaagttat agatgacgat ttcttagttg
    14101 aaggtgactt aagaagagaa attgctttaa acataaagag attaagagat ataaaatgtt
    14161 atagaggaat aagacacgct aaaggtcttc cattaagagg acaaagaact aagactaatg
    14221 ctagaactag aaaaggtcct agaaaaacag tatctcgtaa aaagaaaaaa taaataaata
    14281 ataactagat gaaggaggga aaaggaaaat ggctaaacca aaaaagaaag ttacacgtat
    14341 tagaagaaga gaacgtaaaa atatagaacg tggtcatgct catatacaat caactttcaa
    14401 taatacaata ataactttaa ctgacgttca cggaaatgct atatcttggg caagttctgg
    14461 acaattagga ttcaaaggat caagaaaatc aactccattt gcatctcaaa tggctgctga
    14521 aacagctgca aaagctgcga tggaacacgg actaaaaagt gttgaggtat tcgtaaaggg
    14581 gccaggttca ggaagagaag ctgcaataag agctttacaa gcaactggac tagaagtaac
    14641 tatgataaaa gacgttactc caatcccaca taacggatgc agaccaccaa agagaagaag
    14701 agtgtaatta ataagagtac aataggaggt gtagataaat ggcaagatat acaggtgcat
    14761 catgtagaca atgccgtaga gagggaatga aattattcct taaaggtgat agatgttata
    14821 cagacaaatg tgctatagta aaaagaaact atgctccagg tcaacatggc caaggaagaa
    14881 agaaagtttc taactatgga ttacaattaa gagaaaaaca aaaagttaaa agaatatatg
    14941 gagttttaga gactcaattt agaaatttat atgaacgtgc tgaaaatatg cctggtaaag
    15001 caggtgagaa cttattaagt ttattagaga gaagattaga taatgtagtt tacagaatgg
    15061 gactagcatc ttctagaaaa gaagctagac aattagtgac tcatggtcat ttcactttaa
    15121 atggaaataa agtagacata ccttcattaa tagttaaggt tggagatgtt atagaagtta
    15181 aagagaaatc aagatcttct gcaaaattca aaaatttagt agaagttaac tcaagaatag
    15241 ctcctaagtg gttagaagct aatgtagaag gaatgacagc aaaagtagtt ggtgttccaa
    15301 caagagaaga tatagatctt gagatagcag agcacttaat catagaactt tactctaaat
    15361 aataagcaca cttttaaaaa ttattttaaa attttgttta ccctcagtgg attactgaat
    15421 tttaaggagg gttttgtcca tgatagaaat agaaaagcca aaagtagata tagttgagct
    15481 tagcgaagac tatagatatg gtaagtttgt tatagagcct ctagaaagag gatatggaat
    15541 aactataggt aacgcattaa gaagaatatt attatcatca ttaccaggtg tagcagtgaa
    15601 tgctataaaa atagatggag ttcttcatga attttcaaca atacctggtg ttaaagaaga
    15661 tgttactgag ataatattaa ctttaaaaga gctttcagca actatagatg gtgaaggaag
    15721 tagaacactt aaaatagaag ctcaaggacc atgctctatc acaggtgcag atataatctg
    15781 tcctccagat gttgaaatat taagtaaaga tttagcaata gctacattag atgataatgc
    15841 taaacttaat atggagatat ttgtagataa aggtagaggt tatgtttctg ctgaagaaaa
    15901 taaaacagag aatgttccaa taggtgtttt acctgtggat tcaatatata ctcctgttga
    15961 gaaggttagt tatcatgtgg aaaacacaag agttggtcaa aaaacagatt atgataaatt
    16021 agtactagaa gtttggacta atggtagtat aaatcctcaa gagggaatat cattagctgc
    16081 aaaggtatta gttgagcatt taaacttatt tatagactta actgaacatg taagtagtgt
    16141 tgaaatcatg gtagaaaaag aagaagatca aaaagaaaaa gttcttgaaa tgactataga
    16201 agaattagat ttatcagtta gatcatataa ctgtttaaag agagcgggaa ttaatacagt
    16261 tgaagaatta gctaataaat ctgaggatga tatgatgaag gttagaaacc taggtaagaa
    16321 gtcattagaa gaggtaatac aaaaactaga agaacttgga ttgggattaa aaccaagtga
    16381 agaatagcga gcaaaggagg gataagaatg gctaagtacc gtaaattagg acgtgaaaca
    16441 gctcacagaa accttatgtt aagaaactta gtaacttgct tactaagaag tggaagaata
    16501 gaaactacag tgactagagc gaaagaaact cgtagaatgg ctgaaaagat gataactctt
    16561 gctaagagag gagatcttca tgctagaaga caagttttag cttatgttat ggatgaaaca
    16621 gtagttaata acttattcac agatttagct ccaaaatatg ctgagagaaa tggtggatac
    16681 actagaataa taaaaatagg gccaagaaaa ggcgatgctg ctgaaatggc ttttatagaa
    16741 ttagtatagt aaggaaagga ttaggcttga tggcttaatc ttttttttat ttttttgact
    16801 aaggtatgat ataataatgt gtaaaatgat ttttataagt tattgcttac taaaaggagt
    16861 atgagttaat ggataatata gtaaaggtaa ataatatttc gtttgagtat attacagatg
    16921 aagcaaaact taaagcaata gataatttaa gcctagatgt taaaaaagga gaatttgttg
    16981 cgataatagg acataatggt tctggtaagt ctactttatc taaaaactta aatgctattc
    17041 ttatgcctac tgaaggaaat attcttattg atgatatgga tactaaagaa gaagaaagat
    17101 tatgggatat aagacaaact gcaggcatgg tatttcaaaa tccagataat caaatagttg
    17161 ctactatagt agaagaagat gttgcatttg gacctgagaa cttaggtata gaaccaaaag
    17221 aaataagacg aattgtagag gagtctttaa aaagtgtagg tatgtatgat ttaagagata
    17281 gacagcctca tttgttatca ggaggtcaaa agcaaagagt tgcaatagca ggaattattg
    17341 ctatgaggcc taaatgtata atatttgatg aagcaacagc aatgttagat ccatctggaa
    17401 gaaaagaagt tatgaaaaca ataaaaagac ttaataagga agaaaatata actgttatac
    17461 atataacgca ctttatggaa gaagcagtag aagctgatag agttgtagtt atggaaaaag
    17521 gaaagaaaat cttagaggga actcctagag aagtattcag taaaataaag atgttaaagg
    17581 aaatcggttt agatgtacct tgtatgacag agttatctag tttattaata gaagaaggta
    17641 taaatattag tagtgatata ttaactgtgg atgagatggt gatggaatta tgtcaattat
    17701 agtaaagaat ttaacacata tatataatga agggatgcct tttgcaagta aggcacttga
    17761 tgatgtttct tttgagatta aagatagaga ttttgttggt cttataggtc atacaggctc
    17821 aggaaaatct acattaatac aacatttaaa tggattacta aagccttcct caggagaaat
    17881 atttataaat gattttaata taacagataa aaacttgaat ttaactgaga ttagaaaaag
    17941 agtaggggtt gtatttcaat atccagaata ccaattgttt gaagaaacga ttgataaaga
    18001 tatagctttt ggaccctcta atttaggctt agaggagtct gagatacata acagggtaaa
    18061 agcatcaatg gaagctgtag gacttgatta tgagggattt aaagacaaat ctccttttga
    18121 attatctggg ggacaaaagc gtagagtagc aattgctgga gttatagcca tgaacccaga
    18181 agttctcata ttggatgaac caactgctgg tcttgaccct ggtggaagag atgaaatttt
    18241 taacttaata aaagacttac atgaaaaaaa gaatatgact ataatactat catcacatag
    18301 tatggatgat atggcaaagt tagcaaaaac tttaattgtt atgaatcatg gtagcgtaga
    18361 atttatggga actcctagag aagtatttaa atcaaatgct agtaaattaa aagatatagg
    18421 attagatatt ccacaagttt tagagcttgc attgaaattg agagaaaaag gctttgacat
    18481 cagtgaggat atattgactt tagaagaagc aaaacaggaa atattaaaag ttgtgagagg
    18541 acgaggatta tgttaaaaga tataactata gggcaatact acccaacaag ttctgctatt
    18601 cataaattag atccaaggat aaagcttgta gcgacaattg tatttatggt atctatattt
    18661 gtagtaaata aattttggcc ttatatagta gttttattat gtttattagc tatgataaaa
    18721 ttggctaata taccagtcaa atatattgtt aagggtgtta aacctcttaa atggattatt
    18781 ttgtttacat ttcttataaa tatttttttc ttaccaggtg atgaaatatg gtcatttgga
    18841 tttttagcta ttacaaaaca gggtctaaga caagcaattt ttatggcaat aagattaata
    18901 tttttagtag ttggtacatc attgcttact ttaacaactt caccaataga attaactgat
    18961 ggaatagaga gattgttgaa tccatttaag aaaattggtt tgccagttca tgaacttgca
    19021 atgatgatga caattgcttt acgttttatt cctacattgt tagatgagac agacaaaata
    19081 atgaaggctc agatgtctag aggtgcagat tttgaaagta agaatcttat aaatagagca
    19141 aagaatttag taccactttt ggtaccttta tttgtaagtg catttagaag agctgatgaa
    19201 ttggctatgg ccatggaggc cagatgttac agaggtggac ataataggac taagatgaga
    19261 gagtctgtta tatcaagaag ggattatatg gcatgtgtat ttcaggttgt ttatctggga
    19321 gcaattatag ctactagatt tatagcaatt taattaataa gttatagata taatgaggta
    19381 aagtatgaga aatataaaaa ttaaaataca atacaatggt aagaattatt gtggatggca
    19441 aaagcaacct gattctttag ggatacaagg tacaattgaa agggctatat atgatataac
    19501 aaaagaagaa accagtctta tagggtctgg tagaactgat tcaggagttc atgctattgg
    19561 tcagattgca aattttaaaa ttaattcagg tataagtata gaaagtatac caatggcact
    19621 aaatgccaag ttgccaaaag acatatcagt tatagaagca tgtgaagtaa atgatgattt
    19681 tcactccaga tacagtgcaa aaggtaaaac atataaatat ttagtatata atagcaagtt
    19741 tagaaatcca atacttagtg agatatcata tcaagtaaaa tatgaacttg attttgataa
    19801 aatgtgttca gaagcaaaaa gtttattagg aacacatgat tttaaaggtt ttatgagttc
    19861 tggttcatct gtaaaagata cagttagaac gatatatgat attgatataa gtaaaaaaga
    19921 tgacttgatt acttttgaga ttagtggtaa tggtttttta tataacatgg tgaggattat
    19981 tgttggaaca cttgttgata tgggtcgtgg cagaattaac gaaccatttt tagatataat
    20041 acaatcaaaa actaggtcaa gatgtggaca tacagctcca gcacaaggat tatttctcaa
    20101 aaaagttcat tattaacttg acacaccagg ctacatgtat taaaatatat gagaatgtgt
    20161 taataagtcc acttgccccg gacttgacat aaacggtaaa aaaattttat gaaaagtagt
    20221 taaggaggga cagttatgaa aagttatata gctaagccag ctgatgtaca aagaaaatgg
    20281 tacttagttg atgctgaagg aaaaacatta ggtcgtttag caacagaaat tgcgacagta
    20341 ttgagaggta aacataaacc aacatttact cctcacgttg atggtggaga ttttgttgtt
    20401 gtagtaaatg cagaaaaaat agttttaagt ggtaagaaat tagatcaaaa atattacaga
    20461 taccatactg gttatgtagg tggattaaaa gaaatatctt acagagatat gatggataag
    20521 aaaccagaag aagttatatc acatgctgta agcggtatgt tacctaaaaa taaattaaga
    20581 agcagaatga tgactagatt aagagtattt gcaggtgctg agcacactca tgcagctcaa
    20641 aatccagaag ttttaaactt taaataagat taataggtga aaggaggagt ttataatggc
    20701 taacgttcaa tattatggaa ctggaagaag aaaaagttct gttgctagag taagactagt
    20761 tgcaggtgaa ggaaatatat tagtaaatgg aagagcatta gaaaattatt ttaattatga
    20821 gacattaata agagatgtta aacaaccatt agttttaact ggtaatgaaa ataaatatga
    20881 tgttatagta aaagttgaag gtggaggatt cactggacaa gctggagcta taagacatgg
    20941 aatatctaga gctttattaa aagctgattt agatttaaga cctgctttaa agaaagaagg
    21001 tttcttaact agagatgcaa gaatgaagga aagaaagaaa tacggattaa aagcagcaag
    21061 aagagctcca caattctcaa aaagataatt acttattttt ttggaaaagc aaaatccttt
    21121 atttactgtt attcagaaag tattggtatt ttaaaaatgt tgggttaagt taattattga
    21181 tatataataa aataataaga tatcttatta ggatgttttt cattaagaag aacatccttt
    21241 ttattttgac tatttaataa aaaacatatg ctaataacat ttaa
//
LOCUS       DAGSWI010000063        21276 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.063, whole genome shotgun sequence.
ACCESSION   DAGSWI010000063 DAGSWI010000000
VERSION     DAGSWI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.063"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            113..328
                     /locus_tag="MJF80_002183"
     CDS             113..328
                     /locus_tag="MJF80_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280843.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            334..627
                     /locus_tag="MJF80_002184"
     CDS             334..627
                     /locus_tag="MJF80_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBZ0280844.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(822..1538)
                     /locus_tag="MJF80_002185"
     CDS             complement(822..1538)
                     /locus_tag="MJF80_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBZ0280845.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(1626..2891)
                     /locus_tag="MJF80_002186"
     CDS             complement(1626..2891)
                     /locus_tag="MJF80_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBZ0280846.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            complement(2995..3303)
                     /locus_tag="MJF80_002187"
     CDS             complement(2995..3303)
                     /locus_tag="MJF80_002187"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBZ0280847.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            complement(3370..4425)
                     /locus_tag="MJF80_002188"
     CDS             complement(3370..4425)
                     /locus_tag="MJF80_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBZ0280848.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            complement(4427..4879)
                     /locus_tag="MJF80_002189"
     CDS             complement(4427..4879)
                     /locus_tag="MJF80_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0280849.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            complement(4876..6906)
                     /locus_tag="MJF80_002190"
     CDS             complement(4876..6906)
                     /locus_tag="MJF80_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0280850.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            complement(7162..9396)
                     /locus_tag="MJF80_002191"
     CDS             complement(7162..9396)
                     /locus_tag="MJF80_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBZ0280851.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTASYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGEGTIEIINSKGKPLFIGKNTELIKDLSHKYKDSADEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYHPITIGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            9715..13143
                     /gene="uvrA"
                     /locus_tag="MJF80_002192"
     CDS             9715..13143
                     /gene="uvrA"
                     /locus_tag="MJF80_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBZ0280852.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCWQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTVSIL
                     DGATYYFGKLRRKKPNGNWMLGELYAIAKDKNIDLDIPWNELPREFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKTGSQNKTIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSSPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPSTYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVTEAKTFFSDCSNIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            13367..14161
                     /locus_tag="MJF80_002193"
     CDS             13367..14161
                     /locus_tag="MJF80_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBZ0280853.1"
                     /translation="MIFCNSFITRKSSMNIVVTHTPIVTTLEMQKAYEPLAKHNVNIF
                     AFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYGNTGIGGMFAQYYA
                     CSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHITVKPPKYKGYNEV
                     YDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAKDSKIKDGVNIPTLVFKTLHDRYFK
                     TQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(14290..14811)
                     /locus_tag="MJF80_002194"
     CDS             complement(14290..14811)
                     /locus_tag="MJF80_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBZ0280854.1"
                     /translation="MFSIGGGYAIILLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            14806..15156
                     /locus_tag="MJF80_002195"
                     /pseudo
     CDS             14806..15156
                     /locus_tag="MJF80_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(15304..16734)
                     /locus_tag="MJF80_002196"
     CDS             complement(15304..16734)
                     /locus_tag="MJF80_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBZ0280855.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(17413..17796)
                     /locus_tag="MJF80_002197"
     CDS             complement(17413..17796)
                     /locus_tag="MJF80_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0280856.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDGNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEENLSDDKLDMLEEWVKNWEEDE"
     gene            complement(17890..18279)
                     /locus_tag="MJF80_002198"
     CDS             complement(17890..18279)
                     /locus_tag="MJF80_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0280857.1"
                     /translation="MKQQEVVNKLRRGELMLMEYLWRKNSILSKKEIIEAMKKRYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNAIKEDRKVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKG"
     regulatory      18488..18585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18640..18816
                     /locus_tag="MJF80_002199"
     CDS             18640..18816
                     /locus_tag="MJF80_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280858.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            19042..19242
                     /locus_tag="MJF80_002200"
     CDS             19042..19242
                     /locus_tag="MJF80_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBZ0280859.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     repeat_region   19606..19896
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19672..19700
                     /rpt_unit_seq="ctttacataccacatagttgatataaaac"
     gene            20123..20305
                     /locus_tag="MJF80_002201"
     CDS             20123..20305
                     /locus_tag="MJF80_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBZ0280860.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            20376..21220
                     /locus_tag="MJF80_002202"
                     /pseudo
     CDS             20376..21220
                     /locus_tag="MJF80_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7581 a         3319 c         2516 g         7860 t
ORIGIN      
        1 ctaaattttg atataaagag aatataaaat actgtaaaaa agtgtaatgg ttatttcctt
       61 gaaaagaaag acatactatt aaagcataaa ttacaatata ggagatgatt aaatgagaat
      121 actttcagaa actgaaaaaa tatctttagc agcaattata aaaatggagt ctgacggatt
      181 acttatgcaa agagcaataa atatgttaat atcagatgaa gacctaaaaa gacaatcaga
      241 atctagtata ttagcaacag aaggaagaat taaagctata caacaattta tagttgaaaa
      301 tgaaatacta atttctgagg aggtatagat aacatgagaa atatgataga aaatctaatt
      361 aagaacaata cttatataga cgataaagta ataatgttaa gtatgctttc atctgctaaa
      421 gcatctgcca atatgtatct taattctgca ttgacaagta gtacacctga attaagagct
      481 atatattcag cttcattaac tcaaatggta gaaggtcaca cagcacttac agaattaagt
      541 attaataaag gatgggttaa accatatgat aaacctattc aacagttaac atgtagttat
      601 aaagaatccc aaaatgttat tgactaaact aaataaaata tatttctaac tatttcattt
      661 taattaaata ataaaaaggg gatattcaaa tttttttaag atttcccctt cttctaaata
      721 atctattttt aacataaaat agattgctca caatctaaac atataatttt atatagttat
      781 tattaattat tttatacaat tctattatta acattttttt actacatatt tttataacta
      841 catattttta taaataaaga tatcaaatcc ttatgttcca aaattacaaa atcaggttta
      901 taaaacaact cttctacctc atgtcctcta tggttcaacc atatactctt ccaaccagct
      961 tttttagcac ctaatacatc atttctataa gaatcaccca cataatatgt attttctcta
     1021 tctaaattca taacattctc agctactcta aaaatattag tatctggctt agaaaatcca
     1081 acctcactag aaataaaaat attactttta tcaacccagt tttcaatatt taactgcttt
     1141 aacttcattc tttgatggtt tgatgggcca ttagttataa ttcctagatt aatatttctt
     1201 tctttagaaa aatttaaaat cctctcaact tctggtatta gtgtaatttg tgattgttga
     1261 tatatatact catcttgaaa actttgtgca tctttttctg taatagaatt tcccaactcc
     1321 tcaaaagctt tcattatcct ataaatatgc atttctttga ttggcatttc tccattttca
     1381 gtcatatgaa atacctcatc actatacttt ctactactca tatataaatc ttcaatagaa
     1441 atatccttta ttgaagaaaa cactttatta tatgcagtgt aaaaaggcgt taattgatta
     1501 tataaagtat catctacatc aaaaattaaa ttaatcaaaa tattagtcct cctaaattta
     1561 atagtggtaa gtataattat aactataaat acctagtata acaatactca ccactataag
     1621 tcctattatt tatttaaaat cctcgtagta acacctttta agttatagca ttctgcataa
     1681 tcacgcaaag gacgtttaat atggtctaca acaaatcttt gaccatcaat attattctta
     1741 cttaaattat cataaagcac ttctatttct tttttaacat catcattatt aaatgtataa
     1801 tgtcctgcaa tatctagtat ttcattagat aaaacttcat ccttaaatat ctcttcagta
     1861 gttagatttt tttgttctcc tactacccat tttctccaac gtccacattc aattgcatta
     1921 acaagaagaa catgtctgat attagaagtt ttctcaacta aaccctcttt ttctaattta
     1981 cactcaactt ctgctaattt taaatatgcc ctagtttctt cagtaccata ttgaggagct
     2041 acatttgtag caataatttg agatggtata tgttctaata aagttacatc atctaagtaa
     2101 tcgccattat gctcttttaa accaactcca tatttcttag ccatttgagc taaatcataa
     2161 gcatttctaa aattaaaagt tcctacttgt tctgtctttc tagttaaagt accagtttga
     2221 ccaactataa atgttggcat tggcaaacct ctattatcga gttcaacctt tagtctagtt
     2281 ataaatgttt catacgtctc agttgaagtc agacctccat tggtttcctc tgtaccaacc
     2341 tcataaccta tgtctggtag attaagtctt tttctctcgt tttcacaata ctcaattaat
     2401 tctacagttc tttctagtac tacatctagt ggaattactt ttccaatttc aaatgggtct
     2461 tttgtagggt caatcatcaa taggtcaaaa cctgcttcaa tgtccgctaa ataagatttt
     2521 tttcctaact ccattgcttc atcaacaggc aaatggtcat ttctctcttt atctctttgc
     2581 catggcccac catggtctct acataaataa tataaaccat caaatccaac tttgtcggca
     2641 accttcttaa tatcttttgt aaaagtttcc tgattccaac catttacata accgccacct
     2701 agttcatctg catctacttg atttctactt gcgataaaca ttaaaggaaa atcatcatct
     2761 tttgctagtt caaaacttgc ttgtaacaag tttggtgaca taggaccaat tcctaaaaga
     2821 gttgcacttt tcccctcttc ttgtaagttt agtaatcttt gcacgacagt ctttattggt
     2881 aactttttca tgactatatc tccttttatt ctatttttaa gatattttca aagatgcttt
     2941 taataaaaat ttctttaaaa acatctttga gttatatgta aaataaatca atttttattg
     3001 aaaagtatct tttattttct taattaatgc atcagatttc ttaattatct cagatacact
     3061 cattcttact accttctttc ctttaaaacg ttccatattt ctaatctcaa catcattagt
     3121 caatattaca caagttgctt catcaatatc tttaagtgac aattcattat ctattccaga
     3181 gcttccttga gtctcaacct ttatttctat accaaatttt ttggctgata attcaagtga
     3241 ttcagccgac atgtaagtat gtgctactcc actaggacaa gatgttactg ctacaatttt
     3301 catattctta tctcctttaa atttatagtt ttatatttac ttttttatac aaattccttc
     3361 catttatatt tataattctt caaatattaa ttcaattgat tcatctaaat cttcttcaat
     3421 ttcttcatct tctttcacgt ctttttttac taatttcatt aataccgcaa caactactgc
     3481 tccagttaat actgatgcaa tataccccaa cctattgtca actacaggta acactatcag
     3541 acctccccag ggtgcatgat ttaaacatcc taataaaaaa gccataatat ttcctacaat
     3601 cgacccagcc ataatacaag gtattactct aactggatct gatgttgcaa atggaatagc
     3661 tccttctgta attccaacac atcccattaa tagtgaagct ttacctgaat ctctttcttc
     3721 cttgctaaat ttttttggaa acaataatgt agctaatcct aaaccaattg gaggtataca
     3781 aattgcaacg ccaactccac ccattaaata tggaagtgtg tcaacttgag tttgggcaaa
     3841 agtagcagca accttattta tagggccacc catatctgag gctatcattc ctcctaagat
     3901 tccacctaaa ggaattttag aaacaccaga cattccattt aaccagtttg tcatccatgt
     3961 cataaaagaa gctataggtt ctccaattaa aaatacaatt ataccacctg taatcaatgt
     4021 accaactaaa ggataaataa atatagaacc taaagttctc attgtaggag ctaatttaat
     4081 tttctttaat tgtaaaacaa caaatcctgc tataaatcct gctatgatac cacctataaa
     4141 acctgcattt acttcttttg ctaaatatgc acctatcata cctggagcta atcctggttt
     4201 atctgccata ctaaatgcta tatatccacc taatatagga atgaataaag ccaagccagc
     4261 agctccaatt tgattcagtt tagccaacca gcctgtttca ggaacagcag cttgtccact
     4321 taaagttact gataaagcaa ataatattcc tcctgcaacc acaaatggaa tcatataaga
     4381 tgttgctgtc ataagatgtt tttgcaaaaa ctttaaaatt ctcattttaa tcttcctcct
     4441 ctattttttc ttttataaga ttataaacag tttgttcatc tgaacatcta atttgatttc
     4501 taaaactttc atgcatcaat aaacgtgcta attttgataa aatctttaaa tgaagattat
     4561 tttcattgtc ttttgttact gatatcaaaa atattaaatc cactttttct ccttcttctg
     4621 tccattcaat aggagtatca agctttccta ttgtaataca agagttttta acacctacac
     4681 tttgactatg aggaagacct ataccatgtc caatataagt cgacaatgtt tcttctctct
     4741 ttaaaacatc ttttaaaaat tgatttttat ctattaaata tccttctttt gacactacat
     4801 caactaacga tataatggct tcttcttttg aattaacatg tattttaaat gaacatttta
     4861 cattaactaa tttattcata cagtgacctc cttctcatat ttatgaatta aaacttctgc
     4921 ttcttctttt gtctttgcac tttctaacat agcaataaaa ttagtcttat aaaccatttt
     4981 agttagatta attattgcag gaatttgctc ttttttatct ctgcttgcta ggcaaaatat
     5041 gattttaacc tttttatctc caaaactaac tgcttcttta catattaata aacttatcga
     5101 acttactttg actaatgaac tatcattgcc atgtagtaaa gcaaattcgt tatcttttac
     5161 agcataaaaa cccacatttt ttaaaatatc tataatattc tcagcatatt gtgctgatac
     5221 aaaatcacaa ttagcaagta tgtttgaact aaattttaca gcatcttccc atttcatatc
     5281 tccatcaatt atccttatgg tatcaatccc tattaaatca cttaaattaa gttgtctaaa
     5341 taatattctc tcatcactgt atccaagttc ttgacatata atacttaata ctcttttttg
     5401 ggcatcttct tctaaaaagt ccaacctttt ttttattgac atataattag ttaagacgtt
     5461 ctttcttttt atcccttttt cttcaaactt caaataatcc tcttcttgta aaaatggatt
     5521 tatctcaata attgttttag gcaagtctat tactatcttt gatgttgtta taatgaagtc
     5581 aatactttcc cattctttat aatcttgtaa cttatatgat ggaatactct ctactatatt
     5641 tatctggtaa tcatttacta aactatcctt taacatagca attacaccat atccaaatcc
     5701 acaaactaac aatatatttt tgtagttatt cctctcattt ctatgtatac ttcctaagaa
     5761 atgtattgcc aaaagtattg tttcattttc attctttatt tgacttaaaa ctctgttttt
     5821 ttgaatagaa accttagtag cttcaagtac atattgatat ttatctggta ttacatgaag
     5881 taattcctca taaatctgta tattctgctt gattctctct atcaatgggg caatatgatt
     5941 gagtaatcct ttataaagaa tcatatcttc tttaaagcta atctctaata tttctgacat
     6001 attgtaaatt aagtcctgaa ctataatttc tgtactaatc aaatccatat cctcgtttaa
     6061 acttgcatat ttatttgtaa aaaatacaaa actctttaac agaaaaattt gttccttagt
     6121 caactttttt tgaataattg tttcaaattc ttggtcaaac ttattttcaa aacacggctc
     6181 acttaatgaa gcagattcta aaggatgttt ttcatctgaa tgtatatacc atgtaaaaag
     6241 gaaaatgttt gacacatacc atgtaaaaga ttcatcattt aaagtccatc ccatttgcaa
     6301 taataaattc atagaccaat tatatatatc aatcaaataa atccctttga atattttttc
     6361 aattacattc actaaatact tctcaaatac acttaaatca gatttttcct taccaaaaag
     6421 ataagaatac gattttaata tgttcaatct ttgcttatat ataatgtctt cttctccact
     6481 tattttaaaa gatttatcat aagtaatagt tatttggttt ctttctaatt cacttgacaa
     6541 aacattaagg tcatttttaa ttgaacttct agaaacctga aattctttac ttaatttttc
     6601 taagtttaga ttatctattt taaacattga tataattttc ataatagata ctcgctcagt
     6661 taaggaaaat acatattcgt tattatttat acatactact tggaagtcat caggaataat
     6721 taacacacct ttgttcatct tttgtattac tgacttgttt aatgaacgca aaacaatatt
     6781 taaattattt atatcatatc taacttttct ttcttctata ctaagttgtt tgctaatatc
     6841 cttgtaactt gtcttatttt ttccttttag aaaattcatg atgagtaaca ttctcccact
     6901 tatcatattt tccctccttc caaaataatt agagttatta atatagcaaa ttttatatta
     6961 ttatgtatgt ttcagttttt atctaagaca gtaactatct ttctatattc agaataatac
     7021 aataaagctt tagccacaac ttacaatatt tgaaaattat ttttacaaaa tctgcaaata
     7081 tatttttatt aaatatataa ataaaaaagg caagttatat ttttaattat ataacttgcc
     7141 taaatttttt atcttgtaca tttaataatg gatattttta attttattat tttcatatgc
     7201 aaaattttca aagtcagaaa ttaataatgg ttttgaaaaa acataacctt gaatataatc
     7261 acatcccaca ttttttagga actcaacttg ttctaattct tcaattcctt cagctgttac
     7321 tttcatacct agccttttgc taagttcaat aatagactta ataactacct taccacggtc
     7381 tatattattt ttgctaacaa agaatgaacg gtctaactta attatatcaa ctttaaaatc
     7441 ctttaatatc cctaatgatg agtacccaaa tccaaaatcg tctatagaac atttaaatcc
     7501 attagaatga atatcctcta ttatatctat agcttctgga ttttctaaga aaatagattc
     7561 ggtaagttct aaatctatca gacatgttgg tacattgtat ttattgcata tatgcttata
     7621 tttttctaaa aacaatttat cattcaaatg ttgacgtgat acattaacag aaattctcaa
     7681 cacttcttta ccttctttta tccacttaga aatcaatttg catgtttctt ctagtatata
     7741 taaatctaac ttacatataa atccactttt ttcgaaaaga ggaataaaat cacttggata
     7801 aattatgccc ttttctgaat ttttccaacg cacaagagac tctgctccac taatttttcc
     7861 actttttata tcaattttag gttgtaagca cataaaaaat tctttatttt caagggcttt
     7921 ttccattaaa tttgaaattt ccttttcaga tattaatcgt tttctttcta tatcactata
     7981 aaaagaaaaa tcactttgat gagttttatt aaagcttttt cttgctgtat ttgcatggtc
     8041 ttgtattgta attaaatctt cattatgatt ttcaattgtg taaataccta ctgatagtct
     8101 taaaaagtat ggattttccc tatttttatt aaaataattt gtgtcagctt ctattttata
     8161 taataactcc agggcttcta acactggtct attcttaata agcaaataga aaaggtctgc
     8221 atcatgacgg cacacaaatt cttcattttc atcaatgttt ttatacaaaa tattgtaaag
     8281 atgttttagt gtcatatttc ccttttcact accgaaacaa tcatttatta gcttaaaatc
     8341 ttttatattc attgctatca tcatataagt actatcaggt gattctgaaa ttaaaggttt
     8401 gactagcata ctaaacttag ttgaatttat gccatttgtt atactatcta aatatgcaat
     8461 attttctatt tgatttacat attttcttct tataatatat aaaattaatg ttataattcc
     8521 aataatacat gtaattataa atgtcattgt tattgtaaaa gaatttaact tattaagttc
     8581 accaagtaca gcatcttcag gtactatgag aagaaagtgc caatcattta aacctattgt
     8641 aattggatga taagttaata agcattgtac tccttttgat gaggttattg taatattccc
     8701 tgatttatca tttttaaaat cattaagcat cttttgtgcc caaggttcat ctgcagaatc
     8761 cttatattta tggctaaggt cttttattaa ctcagtattt tttcctatga aaagaggttt
     8821 tcccttacta ttaataattt caatagttcc ttctccatta aagttatcta tattaagaat
     8881 ctcattcata ctttctttat tggatacacc tattaaaata ctagatactt catgattatc
     8941 attaaatata ggtacactat aaagaacaaa acccattgac tctattactt ctgagtattc
     9001 tttaccttca attccttttt tataggaagc agtattcttt aagtcaaact tttttccact
     9061 taaaaactca gctttacctt ctaaatctat tattccaaca tcataatact tataactagt
     9121 ctttctttta gataaatact catatcgact atcaccttca tattgcttaa taaaatctgc
     9181 cattattctt aagtcggata aaataaattt gattttagaa tcaacattca ttacaatctg
     9241 tgtagataca tcgtttaaat agtttttaga ttgtttatta gatatattgt gtagttttac
     9301 ggtattaaaa acagtaatta caaggattga aataataagt gaacataaaa ttatagacag
     9361 aactaaacta tttttcttat accttgataa tttcattttt ttcctccaaa ttataaacct
     9421 attttttaat gtatattaac acttacaaat ttttttacta agaatccttc acactatatt
     9481 atagtattct atttcttaat attcaagaat gctatcttaa catttcatta acattccata
     9541 gataatggtt ttattaaata attatattat atgcaatttg aaactatatt tgtattacaa
     9601 tttatcactg tatcattttt tatttatttt taaattttgg ccaaaaaatc taaattgtcc
     9661 ttgcatatga caccatgtca gggtatatca tccatttaga gaggtgaaat ataaatgaaa
     9721 ataaataact ataaaaatca aagcatcatc acaaatccta aaaaatttga aaataaatat
     9781 caagatttac caaagactcc aattgaatta ttaaaagtgg ttcaaagcct agtaattcat
     9841 ggagaccaag gaaaattata tggtatatcc tttaataaac aacaatctga tgaggaattg
     9901 cttcgtacaa ttcctcaaat gttaaaaaga atatttgaaa taaattctaa cccactaact
     9961 attccaagaa acccaaaaca aaggcttgtt ggaatgtgca gagattattc tttacttctt
    10021 gtatcgctcc taagatatcg aggttttgag gctagaatga gagcaggttt tgctaattat
    10081 tttgaaagtg aacttactta tgaagaccac tggcttgtag aatactatga tactctaaca
    10141 aagagatgga taagaataga tgcacaaata gatgatatac aaaaaaatta ttttcaaatt
    10201 aattttgaca ctcatgatgt cggaaaaaca gatggttttt taactggttc agaagcctgg
    10261 ataagatgtt ggcaaggaca tgctcatcca gatgactttg gatataataa aaattggaaa
    10321 ggttggcatt ctgtaaaggg taatcttcta catgacttta ataatatgat tggtttagaa
    10381 ttgcttccat gggatttatg gacagaatta agttccaaaa aatacaatca attaacaaga
    10441 gcagaaaaaa atttattaga tgaaatggca gaaatattat caagcggaaa tataaaaata
    10501 gaagacctta atttgttaat agaaaaattg cctgaagatt atttgaaatc aatcttttct
    10561 cagttaaaaa tattaggtat ttcggaaata aaagaattag gcaatcctct tgaactagaa
    10621 aagaaattca aatttacaaa atcaataaat aaatcaatta aaaattcact ttgtcataat
    10681 aaaagctcta tctatttaaa aggaggaagg caaaacaatt taaaggatgt tgaggttaca
    10741 attccaaaaa atcaaataac tgttattaca ggtgtaagtg gtagtggaaa atcttcttta
    10801 gctttcgata cgatttatga agaaggaaaa agacgttatt ttgaaaatct aggtaatgga
    10861 gcaaaacttt cagaacagct tcaaaaaccg gaatttgatt tacttcaggg attaacccct
    10921 actatagcta tcgaacagaa aaaaggaagc caaaatcctc gctctactgt tggtactctt
    10981 acaagcattt gggattattt aagaatgtta tttgtatcaa tcggaaaaag ttactgtcca
    11041 tattgcaaaa ttccattaga aaaaaagaat aatacaaaaa attattgtcc acattgtcaa
    11101 actatttttt ctaagataaa tacttcaacc ttcaatgcta actcccatac tggagcttgt
    11161 cacgattgta atggattagg ttttacctat caagttaatc ctcaattaat tgtaaaagac
    11221 ccaactgttt caatattaga tggagctact tactattttg gaaaattgag gaggaaaaag
    11281 ccaaatggta attggatgtt aggtgaatta tatgcaatag caaaagataa aaatatagac
    11341 ctagatatac cttggaatga attaccaaga gaatttatag atgcaatctt atatggtaca
    11401 gacgataaga tatatgaatt tagctttgaa tcaaagggac gagaatcaaa gattagaaga
    11461 cctgcttcag gagcaatcaa ccacattcag agacttttta gagaatctag ttctgaaaat
    11521 aataccttgc atcaatatat gaacaaaatt ccttgcaata catgtggtgg cgaattgctg
    11581 tgcattgagg ctagattcac tacaatcaaa ggttatcgct tccctgaact aacaaaaatg
    11641 acgatagaac agttatggaa ttggttgtgt gaattaccaa atcaacttca aaaaaatgag
    11701 ctctctttgg taaatgatat cttaacagaa ttaaaaattc gagttagtta tctattaaag
    11761 gtaggtctta gctatatatc aacagataga acagctccaa ctttatctgg aggagaactg
    11821 cagagagtac gattatcaag tcagctagga agtgaattag taggcttaac ctacatttta
    11881 gatgaaccat ctattggttt acacccaaga gaccataatt taatcatcaa aatgatagaa
    11941 gagctaagag ataagggtaa tactgttata gttgtggaac acgataaaga tacaatactg
    12001 tctgctgatt atattattga tgtaggtcca aatgctggta caaaaggggg gttcattatt
    12061 gcagaaggta caacccaaga aattataaaa aatccaaact ctataacagg taaatattta
    12121 agtacataca ataaaacagg ttcacaaaat aaaactatac caagtaaatg gttaagctta
    12181 aaaggatgcc atgcaaataa cttaaaaaat attgatgtag aaattccctt aaactgtatg
    12241 tgctctatta caggtgttag tggttctggt aagagctctc ttgtatttca cagtttactt
    12301 cctgctctag aagaaaaatt gaaacaaaaa tctagcccag ataaaaatta tactgaattt
    12361 actggatttg atgctattga tgattttatc ttaatggacc aaacacctat tggaaaatct
    12421 agccgttcta caccttctac atatatcaat atttttgatg aaattcgttc tctatttgct
    12481 gaaactccac aagcaaaaca aaaattattg gatgaaagct attttagctt taatagcaaa
    12541 aaggggcaat gcccaaactg tcaaggatta ggtaaaacaa aaattattct gcaatatatg
    12601 gcagaccaat gggtaacttg tagtgaatgt caaggtaaga gatatcaaaa agaaatactg
    12661 tctatacaat acaagggaaa gacaattgca gatatacttg acatggaagt aacagaagca
    12721 aaaacatttt ttagcgattg ctctaatata tatagaaaac tctccttatt agatgaagtg
    12781 ggacttggat atctaaagct tggacaaaat acattaggat taagtggagg agaatctcaa
    12841 aggataaaat tagcaaaaga attagggaca aaaactaaaa aacgaatgct gtacatattg
    12901 gatgagccta ctactggact tcattttaaa gatatagaaa atttgttaat tacttttaga
    12961 aaacttgtaa atgaacagca ttcactttta attattgaac acaatacaga ggttatacgt
    13021 gcttctgatt ggattatcga tataggtcca gacagtggaa taaatggagg ggaaattgtt
    13081 gcttctggaa ctcctgatga aataaaaata aatccaaata gtattactgg acattttata
    13141 taaaaacaat taaatacaca tgaacttaaa tataaaatta tagaatgtct tatattatag
    13201 aagaattatt ataataggtg tacaataaaa aaattaactt tagtatatta tctgtgataa
    13261 aatatgaata atatataaaa cagaacataa aaattattat attttataat agagaaagga
    13321 aaatcaaatg agttggaaac atataaaaat accaaatgga gatacaatga ttttttgtaa
    13381 ctcatttatt accagaaaat catccatgaa tattgttgtt acacatactc ctattgtcac
    13441 tacgcttgag atgcaaaaag catatgaacc acttgccaaa cataatgtaa atatatttgc
    13501 ttttgatttt tcaggaacag ggaaaagtga tggatatgaa aaagattttt ctcgcaattc
    13561 aattgtaaaa gacttagatt ctgtcattac atacatagaa aaaaattatt cttccaacat
    13621 acatctatat gggaatacgg gaattggtgg tatgtttgca caatattatg catgttcaga
    13681 caataagtta aaaagttttg ctcagtttgc ttgtgttgat tataaaaaca caacaggagt
    13741 aggctatacc tatctcactg ttaaattatt aaactttttc tttaagtttc ttcctaattt
    13801 tcacataaca gtaaaaccac ctaaatataa aggttataat gaagtctatg ataatgaatt
    13861 ttataaaata tttgaaaaga aaaatcctca tatctggaaa aatagtacaa agataattaa
    13921 tacaatgtta gagtgcttta ttgcaaaaga tagtaagata aaagatggag tcaacatacc
    13981 tactcttgtc tttaaaacac ttcatgatag atattttaaa acccaatatt ttgatagcta
    14041 ttataattca ctaaaatgta aaaagaaact tgttgaaatt aataatgttc ataatagtta
    14101 ctatatagat agtgagaaat tttgccaaga agcttatgat tggtttgtag aaaaccagtg
    14161 atataattta aatgttatat gggtttctga ttatcttttc agaatttatt aaaaatccaa
    14221 atagccttac tttattagct atttggattt aagatataaa aaaatagata tcctctactt
    14281 accaaaaact tataaataaa aaaatattcc cagtatacca cttattatta taatcacaat
    14341 aggattaatt ttccacttac gtactatcag tagtgtaaca aaaaagatta ttagtgcagt
    14401 ccagtttagc atctcaaata tagaattgaa gtacacttta ttcgtcccaa ataatgcaag
    14461 gattataatt gtagccgcag ctgagataat taaacctaat gaagctgatt ttagaccatt
    14521 taatatttca aatatataag tagagttctg atgcttttga aaaaaattat ataaaaatag
    14581 agatattact acaccacata atacacaacc taaagtggct gcaactgctc cccaaaaccc
    14641 tccaatttga agtccaacaa aagtagaagt attaacagcc agtggccctg gtgtcatttg
    14701 tgaaatagtt ataatatctg taaacatttt ctcacttatc catccagttt gatttactac
    14761 tttctcttgg attaaaagta tgattgcata accaccacca atactaaaca agccttatct
    14821 ctacgacagt tatgcacgtt tccgttattg ttacgtgagc aaggaagtgc cattcgcaat
    14881 actttggaca gtgtattatc tctttcaaac caaaaagctt atcctgtttg ggaaagtgtt
    14941 aattcttttg cacttattaa agctgcacaa gcagggttag gtataaccat tttgccagaa
    15001 aatttattat tagattcact cttacataaa aaattgcgat tgattgaatt agatgggata
    15061 gatatggaaa ataaaatgtt agcaatatta cataaggata aaaatattac acaacctttg
    15121 aaaataatat tggataatat aagcaatgta ctttaattta aaggtttaat tttttaacat
    15181 tctatttttt agcataaaaa gtgtgtacta ttccttaatg ttgtgatttt aattatcatc
    15241 aatttttaaa ataatacaca ctttatttta agtacaatct aaacttaaat aagataaaag
    15301 tttctattgt atatgtttta aattaatact atattcttgt ttaccttttt caattttaaa
    15361 tttatcattt ttattaggat aaatagttac aacattatct ttatactctg attcatcaca
    15421 ttttatatta attggcataa cttcagcttc accatataca tagctaacag gcttattatt
    15481 attcattaca acaatctgca ccatgtcatc accctttgga gatgatatat ttctccattt
    15541 atatcctatt gttttatata tatcatctgc agatgttcca gatttaaaaa catatatttt
    15601 atcccattca ggagtcttta cttctgataa tcttatttct ttcttatctt tacttacttt
    15661 attaattgaa gaaataaatt ctccaaattt atcactttct tgattttttt caaatttaat
    15721 taatttacta gatttgtcaa ctatattcca tttatcattg tttttttcta atttaaaatt
    15781 aatcttagaa atacattccc catgctttcc tggctcagta actataacat tttctcctga
    15841 tttattttta taatcatgtt gctcaatttg aacatgatta tgacctgcaa ctatagcatc
    15901 aattccatca acttgttgag ctaagtcttg tattctattt cctgggtttt taggtttctt
    15961 aggtttctca cctgtatgtg ccacagcaac tataatgtca ggcttctctt tttccattat
    16021 accaacccac tttttagcat cttctactaa atcattcata tacaattcac cattgtaacc
    16081 ctcttggtct tttagttcta aagacttaga gtcaacaagt tttccatctt tccactgttt
    16141 cctctcacca acttctttta tagtaagccc taaaattcct agttttacgt ttccttcagg
    16201 tgtattaata ttttttatta tgtatggttt tgtataacta tctccatttt ttttatacgt
    16261 atttgccgat aaaatattta ttttttgctt ctcaaaatca gatatcatat tatctagata
    16321 aaatttattg ttggatataa attcatgatt cccaagaact gcaacatcat aacctacctc
    16381 tttcatatct tttgaaatag gcgtctcaat ataactttca gtgttattat caccatcttt
    16441 atatctagca ctaaaataac tatccatgct agaaccaaac acttctccac tatcaaaaaa
    16501 atctcctgca tctactaaag ttagattttt atcttttttt ctttcttctt ttacataaga
    16561 agttaactca taaggtataa ctccatgtaa atcagtagtt gctaatacgt ttatttctgc
    16621 ggtttgtttt gaagcacatc cagacattag taatacaagt gaaaaagtaa ttcctataat
    16681 tattttattt ctcataatcc ctccagcttc tataaatatt tgatttagta tcattgacaa
    16741 ctcaattctt ctataatatc aatattcata cattaatata caaaagtcat ctatatgttt
    16801 tatagacata catttcatag acataaaata cttttatata cttctaataa ttataaatct
    16861 tttctattat atcatcaagt ttatttatga ttataccaaa tggttttcta attaagtgaa
    16921 tataaaaagc tatgaacaat attactcaaa ttaccatcaa gagtaacata aatacatatc
    16981 tccatcaatg attaagaaat tactttttta ttgtatcaat ttgtctttca gagcatgaca
    17041 caaaaggaac taaaagataa caacatcatc agtgttaaat acttcgagtt tcagagcaaa
    17101 ggagttctaa aacaaaataa aaacaccccg ctgaatttat cattcagcag gatgtttttt
    17161 gcttattaat ccataagata ctggcaaata ttgatagtat caaaccagta taacatagaa
    17221 gtaagagaga cctcaaatcc tgtatttatg tggatttttg gcattctata ttttattccc
    17281 attctattga ttttcttttt aatctgataa aactttatct attacattaa ttttcttcta
    17341 ttatttctat attttctata ttttttaaca cgctataaaa catattagta tcattttagt
    17401 atcacattca ttttattcat cttcttccca attttttacc cattcctcca acatatctaa
    17461 tttatcatca cttaaatttt cttcatcatt taaagcagtg aaaaaacttt ttaaagaatt
    17521 attatgaaaa aaactaaaga atttttttgt ttcaactttt aaataatctt tttctcttac
    17581 agcgatagta taatatgtac acttgcttgt tttttgagaa tacaaatagt ttttctttgc
    17641 aagcttagac agtattgtta atgtcgtatt tttattccag tcataaattt gtttcattgc
    17701 ttccacaact tctcctgatg ctaccttatt tccatctaat ccccagataa acttcattac
    17761 ttttaactca gaatctggca atttcttgaa tcccataagc cacaccccct ttttgtaaat
    17821 ttatgtatgt ttatgtatat ttaaatatta taacaaattt attttaaaaa taattaatat
    17881 atatataaat tacccttttc caattttttc catattttct atatattttt ttatttgttc
    17941 ttctgtcatc ttttcttttt tatgcatagt ggtaaacatt cgtataaaaa aactatcata
    18001 tttagttacc tttctatctt cttttattgc atttaaatat tcttttttag ttactagcac
    18061 ttcataacta aactgaaaac ctatcctctt cttttttaat gttttcattt ttactaatct
    18121 ttttaataga atttctgtag tacttttcct ccacttatat ctttttttca ttgcttctat
    18181 tatttctttt ttagataaaa tagaattttt cctccacaag tattccatca acatcaattc
    18241 tcccctgcgt agtttattaa ctacttcttg ttgtttcatt ttatgtcact ccctcaaatt
    18301 gttctaatct aaatcaataa taaaaccttc tgatttcaat tgatacaggt aagttttacc
    18361 aaatattcta cgcatttagt ctgttttctt agaagaaaat aactttcata tctataaaat
    18421 tattaaattt tcaaacaatt ttgttgaata ataactaatt ctattgtatt attttagttg
    18481 tagaataaaa ctaaatcggc aaaactagag aaatttagtg acgcaaagct atagggacta
    18541 agacttatat aaatatctta tgagttatgt cagccagttg ccaaagagat agtgttcttt
    18601 ttgtttttat gaaagttttt tagggggata ataatttata tgtttggaaa taaaatggat
    18661 aaatgtacac atgtattgac tgcttatatt agcagttcgt atgattattg taatttttta
    18721 gatacacagt tagatgattt tatattagag tacggagaaa atgtagtaga gtcttgttta
    18781 caccaagtaa tggtattggt aagtaagtat aattagatag acattgattt ctagaaaacc
    18841 ctttttagtt ttatctacat ttatattaat atatagcaat gtaaaacgtt taaatatatt
    18901 tttaatatta agaataatta aaatgttaac taaaaaatat aattaagtag acatttctat
    18961 taatctatga tacaatagta tttgtggtaa tacacaatta aatcaagtat atcttgatat
    19021 aggttaatag gagtattttt aatgaataat ggaatagtga aatggtttaa taatgaaaaa
    19081 ggattcggtt ttatatcaat ggaaggtaga gatgatgtgt ttgctcattt ctcagctata
    19141 caaacttcag gatttaaatc attagaagaa ggtcaacaag taagctttga tatagttaaa
    19201 ggtgctagag gtcctcaagc agaaaatata actatattat aatatataga tttttcggga
    19261 aagactcttt ttagagtctt tttttattgt taatatatgt aagataatca ttggtgaagt
    19321 aaataaggta tattgagtaa tgttcttgat agtttatatg ctaatgaatt tcaaaaaaat
    19381 aagcactcct ataaaaagag tgctcttaat gtaaatataa accttcattg gtccagccta
    19441 ataccccgta ttctccaagt tctaactctt ttttatatac attatcactt tcagccatat
    19501 ctaacacaat catttcttct ttagaatcaa tttgtaaaat atgaggactg tgtgttgtga
    19561 caattatttg tgtttttggg agaattgttt ttaatgtaat aaaaatttta cataccacat
    19621 agttgatata aaacttaaga ctagcagact cataagcaat tagttgcctc tctttacata
    19681 ccacatagtt gatataaaac tttaataata aggagagaaa aaatgaatat ttatgacttt
    19741 acataccaca tagttgatat aaaactaatt ggagtccaat ctaatttaat agaaggaata
    19801 ctctttacat accacatagt tgatataaaa ccctattctt atatttcaaa tcctttgagt
    19861 gcttatgctt tacataccac atagttgata taaaacccca aaataaactt agcatctcca
    19921 atgtttacac atatacaatg ctcttaaaat tgcagtgaac gcgcaatagt gcaattgata
    19981 atacttatta tatattctca atgccttata tttcaactgc taaacctcaa tttgtcacaa
    20041 atatcgctca ctgcaaaatt tctatatttt tattatatca taacatataa ttaatatttt
    20101 caattaattt ttacatttaa ttatgtttaa attctataat aatgcaactt taatgtttct
    20161 ttctggaact aatataaaaa ctatttcaga aagattaggt catgcagata taaaaataac
    20221 tatgaatagc tactctcagt ttttagaaga gatggacaga gaagcatttg aaaatttgag
    20281 caaaatacta tttattatat tttaaagaag atgacttatt caatatttga tgctagaata
    20341 taattaataa aaatttaatt ataggtgatt gtattatgaa tattattaaa agcataactt
    20401 tactcttaac attattaatt gtgatattaa tgacaggctg ttccccaaga gaaagtgcta
    20461 gtgaagttgc aaaaaaatat attgataaga tgaactctgg tgactatgaa acagcatata
    20521 gtttattaag taaagatagt aaaaaaaaaa ttagtataga aaatcttgaa gaatttcaaa
    20581 atattttagt tagtacaaaa agaataatta gttataaaat aggtaaagaa aaaatcataa
    20641 aaaaatataa acatgatgga aaagaatata aaaatgttgt taaattagaa gaaactttta
    20701 atataaaaaa ccttctttat aaaaaagatg attctctaaa agtaaataga tattttgtag
    20761 ttgaaaataa caaatacaag ctattattgt atgaaagttt caaaaaagaa tttggtatta
    20821 atagtatgga ccttgctcaa cttaagctta atgattttgg actaaaatac tttaaagaag
    20881 ttgaccttat actaaaaaaa gctattgcta ttaatcctac tgattcaaag ctttattatg
    20941 cccaagcatg taattatatg aattttgatg gatttcctgg ttattcagat acaacatata
    21001 atgctttaga ttcaattaat aaatctttaa agtatttaga taaaaatgat tctgactaca
    21061 aaaaaatgca agtaatatat ataacttaaa aggagttatt cttatgtttc acaaaagatt
    21121 tgatgaagca caacaatatt taaataaagc cttatacttt aacaaagata atgaagaccc
    21181 aaaatataat ctaaatacaa taaaaaaaca tctaaactag atatttaatt tttgaattta
    21241 taaaatattt ttttatgtat aattcagcac tgaatc
//
LOCUS       DAGSWI010000064        21252 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.064, whole genome shotgun sequence.
ACCESSION   DAGSWI010000064 DAGSWI010000000
VERSION     DAGSWI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.064"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            157..1479
                     /locus_tag="MJF80_002203"
     CDS             157..1479
                     /locus_tag="MJF80_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBZ0280861.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            1776..3137
                     /locus_tag="MJF80_002204"
     CDS             1776..3137
                     /locus_tag="MJF80_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBZ0280862.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            3210..4604
                     /locus_tag="MJF80_002205"
     CDS             3210..4604
                     /locus_tag="MJF80_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0280863.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            4666..5136
                     /locus_tag="MJF80_002206"
     CDS             4666..5136
                     /locus_tag="MJF80_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBZ0280864.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            5139..7355
                     /locus_tag="MJF80_002207"
     CDS             5139..7355
                     /locus_tag="MJF80_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBZ0280865.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSLDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKIFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGYVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            7438..8109
                     /locus_tag="MJF80_002208"
     CDS             7438..8109
                     /locus_tag="MJF80_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBZ0280866.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            8472..9686
                     /gene="dpaL"
                     /locus_tag="MJF80_002209"
     CDS             8472..9686
                     /gene="dpaL"
                     /locus_tag="MJF80_002209"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBZ0280867.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            9813..11000
                     /locus_tag="MJF80_002210"
     CDS             9813..11000
                     /locus_tag="MJF80_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBZ0280868.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMASVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFVGTEGYYGILKSV
                     YGK"
     gene            11133..12503
                     /gene="hydA"
                     /locus_tag="MJF80_002211"
     CDS             11133..12503
                     /gene="hydA"
                     /locus_tag="MJF80_002211"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBZ0280869.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            12521..13927
                     /locus_tag="MJF80_002212"
     CDS             12521..13927
                     /locus_tag="MJF80_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0280870.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            13999..14445
                     /locus_tag="MJF80_002213"
     CDS             13999..14445
                     /locus_tag="MJF80_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBZ0280871.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            14484..15278
                     /locus_tag="MJF80_002214"
     CDS             14484..15278
                     /locus_tag="MJF80_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBZ0280872.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNIIGGECDGK"
     gene            15268..17559
                     /locus_tag="MJF80_002215"
     CDS             15268..17559
                     /locus_tag="MJF80_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBZ0280873.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            17560..18873
                     /locus_tag="MJF80_002216"
     CDS             17560..18873
                     /locus_tag="MJF80_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBZ0280874.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            18909..19994
                     /locus_tag="MJF80_002217"
     CDS             18909..19994
                     /locus_tag="MJF80_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBZ0280875.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            20224..21189
                     /locus_tag="MJF80_002218"
     CDS             20224..21189
                     /locus_tag="MJF80_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280876.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEITVMKEDMSEVKQEINVLKGNMSEVKQEMIVMKEDMSEVRQEINIMKEDMSEVRQ
                     EINIMKEDISEVRQEMTVMKEDMSEVKQEINVLKEDMSEVRQEINIMKEDMSEVRQEM
                     TVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNFMQNKINNINEDMNG
                     IKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLLHNSEINKATHDNIE
                     HNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSVK"
BASE COUNT         8235 a         2376 c         4346 g         6295 t
ORIGIN      
        1 aaaacaatta tgttaaaaaa ttggcattat aattgcttca tattaagttg agatgacaaa
       61 ataaaaaaag aaaggatgtt gtgtgagata ataataactg tcattaaaaa atagttggga
      121 gtgataacct tgagtaaata tcaaaagaat gtttctatgt ttgatattga tggaaggcca
      181 ccaattttaa agtgtttgcc actatcaatg caacatatac tagcaatgat agttggaact
      241 gttacagtac ctatagtcgt aggaggagct gtgggtgtta gccctacaca aataacactt
      301 ttagtgcaat atgcattaat aatagctggt atatcaacac ttatacaact atatccaata
      361 ggaaatattg gttcaagatt gcctataatt tttggagttg gttttactta tgtaccaaca
      421 cttgtagcag tcggaggaaa ttatggatta gcaagcattt tgggtgcaca attaataggg
      481 ggagtggcta cagtattaat agggatattc ataaaaaaga taagaaaatt ttttccgaat
      541 atagttgcag gaacagtggt ttttacaatt ggattatctt tgtatcctat agcaataaat
      601 tatatggcag gtggtgtaaa tagtcctact tatgcctcta tgcaaaactg ggcagtagcg
      661 gctataacat tagtggtagt tataggtctt agtcaatttg caaaaggata tgcaaaattg
      721 gcagcaatat ttttgggaat tatagttgga tattgtgtat ctttagttct gggtataata
      781 aattttgacc ctattagaga ggcaacttgg tttgcgatac ctaaaccgct tgaatttggg
      841 atggaatttg atatgcctgt aataatctca gtagtaattg taagtataat caatgctgtt
      901 caagcaattg gagatttatc agctactaca atgggaggca tggataggaa tatcacagat
      961 aaggaattat caagtggagt aataggaagt ggaatttgta ctatgatagg agctttgttt
     1021 ggaggtttac caccatcatc atatagccaa aacgtgggga tggtagctat gaacaaagta
     1081 ataagtagat ttgtgcttgg aatagctggt atatttatgt tattgtcggg gtttgtgcct
     1141 aaatttggtg cactaatgac tactatacca tattctgttt taggaggagc tacaatatct
     1201 atatttagta tgataacaat gacaggtata aagttaatta ttacagaaga gttgtcatca
     1261 agaaatatta ccattgttgg tttggcaatt gccttaggca tgggaataac atcagtacca
     1321 gcagcccaag aggcatttcc ttcatttgta aagttagtat ttggagaatc tccaatagta
     1381 attgcaacat tggttgcatt cgttttaaat ctaataatac caaataaaag tctagaggat
     1441 gaggctaaag aaagagctgt tatggaagag actaattaag atataataat ttaaaagaat
     1501 tgtttaaatt atttagtatg agattgttag attatttttg atagctattc attttaataa
     1561 aaaatttaac aaataaatat ataccaggca attaatgttc atttaagttg aaaatcgggg
     1621 agggaatatt tagaaatttg ttaatttttt ataaaaatag ctcagtttag ctgtgtttat
     1681 cggttgagca ataaattcat ggtcataaaa atatttatag aaaataatta taaaaatatt
     1741 tagtcaagat atttatgtat gggaggattt attttatgaa aacacgtaat acttcaaaat
     1801 atgatgtcga tgggatacca ccactaaagg aatcaatacc tttggcctta caacatctgt
     1861 tagcaatgat agtaggaaat atggtacctg cgatattaat agcaaacgtg gtaggactta
     1921 accaggggca agcaacaatg ttgattcagg gttctatgtt agcagcaggt ttagcaacgt
     1981 ttttacagtt atatccaata cctttattta aaggatttaa gttaggttca agattacctg
     2041 taatgatggg aatgagttat gtatttttag gagcttgtct ttcagtagca gctaaagatg
     2101 gtttagcagc tctatttgga gcacagattg ctgctggggt aatcgtattc tttgtaggat
     2161 ttggagttaa gaaaattaga catatattca ctccaatagt ttctggaact ataatagcat
     2221 gtatgggact tggactattc gcaacagcta taaagaattt agctggagga gaaggcacac
     2281 aaacatttgg aagcccaata aacttcacag taggagttat agttgctttt gtaataataa
     2341 tgataaataa atacggtaaa ggtttagtaa aaaattcttc aatattaata ggaatattag
     2401 taggttatgc tatatcttta gttttaggat tggttgattt ctcagccgtt caaggagcag
     2461 ctatagtatc tttaccaaca ccagcagctt ttggattaga atttagacca gagttaatag
     2521 taatgtttac tattatttac ataataggta tagctgatat gatgggtgcc tgtacaatag
     2581 ctacagtagg agcaatggat agagaggtaa ctgacgaaga attagcatca gttgttttag
     2641 gaaattctat aacttctata atttcatcac tatttgcagc tcttccaact ggagtattta
     2701 gccaaaatac agtaattgta tctatgaaca aagttacaag tagatttgta atagcactag
     2761 gggcacttgt attattacta gcagggatat ctccagcatt gggtgcaata atgacaacta
     2821 taccatcttg tgttgttggt ggagcaactt tagtagtttt ctcatcaata gcaatgtctg
     2881 gtttctctat tatgagtatg gatggattca ctgaagaaaa caatcttata gcaggtgtat
     2941 ctatagcaac aagtatgggt cttacaactg ctccacaagt tttagaccaa ttcccagaaa
     3001 caattggaac tgtccttgga gggtcaagta tagtttctgg agcaataata gctatattac
     3061 ttcaaacatt atttaaatta aagtcacgta aaagtgctga aaacgttact tcaaatcttg
     3121 aggaaaatat tggataaatg tattagatat aatatgagaa aaccatatgg taaaatgttg
     3181 taatgaaagt attcaaaggg ggatttaaga tgattgatat actaataaaa aatgcaataa
     3241 ttgttacagt taataaagac agagatgtta tatttgatgg agcaatagct attcaaggtg
     3301 acagaatctt ggacatagga aatacagttg atattgagcc taagtatctt gatgctaaaa
     3361 aagttataga tgctaatgga aaagtaatat tccctggctt tataaatact cataatcacc
     3421 tgttccaagt gttattaaaa ggtttaggtg atgatatggc acttcatgag tggttaaata
     3481 caatgatgtt tccatcagca aaattcttaa cagaacaaga tacttatgat gctgcaatgc
     3541 ttggatgtat ggaaggttta aaaagtggta taactacaat ggttgattat atgcatactc
     3601 acaatagacc aggacttaca gatggaatag taaaggcata taaagattta ggtataagag
     3661 gggttgtagg tagaggttgt atagatttag gtattcacaa agagcttata gaagatgtgg
     3721 aaacggttga aaaagatttg agaagggtat tcgaaaaata tcataattca gaaaatggaa
     3781 gaataaaaat ctgtgtagct ccttcttcaa tgtgggctat atctgaagaa atgggtaaga
     3841 tgctttggaa tatagttaaa gaatatgatt catattttac agttcatata tctgaaacag
     3901 aatttgcaag aacagcaaca aaggatatac atggagaaat agatataaaa ttacttgaaa
     3961 aatggggtat agtaggtcca gaagttgtag cagtacattg tgtatgtata actgatgaag
     4021 acatggaaat gcttaaaaag tatgacataa aagtttctca taatgtagca agtaacatgt
     4081 atcttgcttc tggtgttgca cctgtgccag aaatgttaaa aaaaggtata aatgtgagtt
     4141 taggtcttga cggtgcagca agtaataacg cacaagatat ggtagagctt atgaaattaa
     4201 cagctttaca acacaaagta aataattgtg accctctggc aatttctgca gagaaagttc
     4261 ttgaaatggc tacaattgag ggagcaagaa ctcttagaat ggaagatgaa ataggttctt
     4321 tggagattgg gaaaaaagct gacctagtaa tatttaactc aatgctttct ccaaaatcaa
     4381 tacctcttca caatcctgta tcaactttgg tatactcagc tagtatgcac aatgtagaga
     4441 gtgttttagt tgatggaaat gtaatacttg aaaatggaaa agtaactaca attgaaagtg
     4501 aagaacaagt gttgcttaat gcacaacatg cagcagaaga attatgtaag agagctaatg
     4561 taactaatag aatggaagga cataagtgga atagcttata ctaatattaa aataagattg
     4621 taataatatt ctattacaac attagtttat ctgtaggagg agaaaatgga aaaaataaaa
     4681 ttaaatatta atgattacat gtatgaaata gaagttgaaa agaatgaaac tcttttgcat
     4741 gtacttcgtg aaaaattaaa tctaactgga gcgaaacaag gttgtaatag tggcagttgt
     4801 ggagcatgta aagttttgat agatggagtt gacatgaaat cttgtaaact tcttgcaaaa
     4861 aactgcgaag gtaaagttat atacactata gaaagttttg ctaatggaga aaatcttcat
     4921 ccaatacaag aagcttttat agaagtcgga gctgtacaat gtggatattg tactcctggt
     4981 atgattataa caacacaagc tcttttaaat agaaatgcat ctcctaatga agatgaaatt
     5041 agagaagctt ttaaagaaaa tctctgtaga tgcacaggat atgtaaagat tatagaagct
     5101 gtaaaattat ctgcagctag attagggggt agataattat ggaaaaggta ataggaaaat
     5161 cataccctct aaaagatgca gctttaaaag ttactggtca aatgaaatac gtggctgata
     5221 tgaaaccaca aaatgtactt tacgctaaga tgcttttaag ccctgtagca catgcaaaaa
     5281 taaaaagttt agatacaagt gaagctgaga aagtagtagg tgttagagct atagctactt
     5341 atctcaatac atcacaaaga aaatacaatg gttcattaag attttatgag cacaaaatac
     5401 ctgaaaatga agttatattt tcagatacag ttagatttgt tggtgaccgt gttgcagcag
     5461 tagctgcaga gactccagaa atagcagaga aagctgtaaa acttataaaa gttgaatatg
     5521 aagaattgcc agctgtattt actatacaag atgcaataaa agaagatgct atctgcatac
     5581 atggagattc aaatatagtt tctcaaaaca acataaatgg gggagatgta gaaaaaggct
     5641 tcaaggaatg tgactatata tttgaagatg aatactctac tccagcaata catcatagtg
     5701 caatagagac tcatagtgta attgcacact atgattataa tgataaatta actgtacata
     5761 caccttgcca aaatacattt ggatttagaa taatactatc tcaaattttt gggatgtcat
     5821 taaataaagt aagagttata agacctgcta ttggaggctc atttggtgga aaattagaac
     5881 ttacaattga accagtagca gccgcattat caaaaatgac aagaagacca gttaaaatgg
     5941 ttcttacaag aaaggaatct atgatatcga caagaactcg tcatgggtct tttgtaaaat
     6001 taaaaactgg tgtaaataaa gatggtgaag taatagcaca agacataaaa atatatacaa
     6061 atacaggagc atatgcatca agtgctttaa atgttatagg agcacttagt cataaagtat
     6121 ttaaagttta taaaatacca aatataaagt ttactgggat gcctgtatat acaaatacac
     6181 caatagcagg agctatgaga ggctatgggt cacctcaaat atttatggct caacaagctc
     6241 aatttgcgaa aatagcaaag gaaataggta tggatttagt cgattttcaa aataaaaatg
     6301 cagtagagcc agatgatgtc gatataatct tccacggttc tcttggaaat cctagagtat
     6361 tggattgtat agagcaaggt aagaagatat ttaagtggga tgaaaagaaa aaacttccaa
     6421 aagaagaagg aaaatattta agaggtatag gtatggcaat aggtgcacat ggtaatggag
     6481 tttttggtgc acatagagat gttgttacat tgactcttaa attaaatgaa gatggtacgt
     6541 tgacactact aacaggagct catgatatgg gtaatggtgt tgtaactatg caaacgatga
     6601 tggtagctga agttttagga ataacacctg ataaagttga tacatttgaa actgatacgg
     6661 atgcatgtac attcaacctt ggtgactatg caagtagagg ggtatttgta gaaggtggag
     6721 gagctaaaaa gacagcagag aaattgaaaa atcttatatt agaagaagca gaaaaattac
     6781 ttgaaacatc aaaagaagat ttatatctag acaatggata tgtaattagt aaacttgatg
     6841 aaaatgtaaa agcatcactt tctgatgtcg cagtatatag tcaaagtaaa tcattaagag
     6901 agttaactgt tgttgaagat tattcttcac cagctggacg tacttcttat ggtgtacatt
     6961 ttgctgaagt tttagtagat aaagaaacta aggatataaa agtagttgat tttgtagctg
     7021 tacatgatgt tggtagagtt ataaatcctc taaatttaga aggacaatta gaaggtggga
     7081 ttcatatggg tctaggatac gcattgagtg aagaattggc atttgatgaa caaggtagac
     7141 ctaaagctac tgattttaaa acatataaat tatttagaac tgtagatatg ccaaaatgtc
     7201 aagttgcatt tgtagatggt tatgaaaaag ctgggccttt tggtggtaag agtattggag
     7261 aatgtgccgt tgtaccagtt gcgccagctg ttgcaaatgc agtatataat gcaactgatg
     7321 tagagataca ttcattacca ataaaattaa aatagtagaa attaaagcag aaataacaat
     7381 ttattgtatt tgtaaataga taataaaaat agatttattt ggtgaggaga tattattatg
     7441 gcaaagatat taggtatttg tggaagtcca cgtaaaggag caacagaata tgcagtgtta
     7501 gaagctttga aagaagcaga aactatacct ggtatagaaa ctgagtattg gtcagttaga
     7561 gggaaaaaaa taagtccttg tgtacattgt gatgcatgta taagaaaaga aactatgtgt
     7621 attataaaag atgatatcca agaattagag caaaaaatat tggaggcaga tggaattata
     7681 gtaggttctc cagtttatga tatgaatata actgctcaac ttacagcagt atttaataga
     7741 cttcgtccaa tgtatttagt tcatccagga aaacttcaaa ataaggttgg tgcagctata
     7801 actactggtg gaactagata tggtggccaa gaattgacaa aacttcctat aataaatttc
     7861 ttcctaatgc atgaaatgtt agtgtctgga ggtttaggtg gatgctatat aggtggtaca
     7921 atatggagta aagatggaaa agcaaaaggt gcccaagagg atgagacagg tatggacaca
     7981 gtaaaaagac ttggaaaagg tgcagcagag gcagttatgg tttctaagtt tggtcttgaa
     8041 aaatggaatg tcgtaaaaga agaattagat gttaaaaaag atgaaaaatc acctcttaga
     8101 gaccactaaa gatttattat attattgaat atatacaaat agtgatgtaa tagattattg
     8161 catcactatt ttatgtttta atttagattt tataaatttt atattaaaaa atatagaaac
     8221 aaagtgtttt gagatttatt tttaaatttt aaaaatgaga attaaaaagt gagaagatag
     8281 aaaaggtttt ttagttatta aattctacat acattgtatg aactaaagat agaaaagaaa
     8341 aaataataaa agcattgcaa tttctatact aaagagtcat ggaaaaatat ctagtatata
     8401 tttagtattg gcatgttaat tgcttatata ttaaagtata aaaatttaac aataatttgg
     8461 aggtatttta gatgttaaaa gaaattaaat ggaaagtaaa taatttgcca aagggcgata
     8521 aggaaaattg tatcaaattt ttaaatgaag aagaaataac taaggttaga aattttcaca
     8581 aaagctttcc tcaatataaa gaaactccat tagcaaacct agaaggttta gcaaaaaaat
     8641 taggtgttgc aggagtatat gtaaaagatg aatcttatag atttggatta aatgcattta
     8701 aagtattagg tggttcttat tctatgggta gatacttagc tcaaagatta gatacagata
     8761 tatctgagtt agggtatgac aaattaactt caaaagaaat aaaagaaaaa ttaggagaaa
     8821 taactttctt tacagctaca gatggaaatc acggtagagg agttgcttgg acagctaata
     8881 aattaggtca aaaatctgtt gtattaatgc caaaaggttc ttctgaattt agattaaata
     8941 aaattaaagg agaaggtgca gatgctagta taacagactt aaactatgat gatgcagtaa
     9001 gattagctaa tgattatgca gaagctgatg accatggagt aatggttcaa gatacagcat
     9061 gggacggata tgaagaaata ccagcgtgga taatgcaagg atatggaact atggctcaag
     9121 aagctataga gcaattaaaa gaatatggag tagatagacc aacacacgta tttgttcaag
     9181 caggagttgg ttcacttgct ggtgcagttc aagggtatgt tgcttcaata tatgatgaat
     9241 gcccaataac agtagtagta gaggctgacg aagctgattg ttactataaa tcagcagaag
     9301 ctggagatgg aaaaccaaga tttgttggag gagatatgcc aactataatg gcagggcttg
     9361 cttgtggaga accaaatact ataggatttg aagtattaaa aaatcatgca gcagcatttg
     9421 tttcagcacc agactgggtt tctgctaaag gtatgagaac attaggtaat cctttaaatg
     9481 gtgatgaaaa ggtaatttct ggagaatctg gagcagttac aactggtctt ttagtagcag
     9541 ctatggaaag agaagattta gcagatttaa gaaaagattt aaaattagat gaaaattcaa
     9601 gaatactttt aataagcaca gaaggagata ctgaccctga caaatataga agtatagttt
     9661 gggatggaga atatccaagt atataaggaa ttactaaaat gcaattatta gatattggtt
     9721 tacatattta gaagatatat aagttctaaa atttagaaaa tatataagta tataaaatac
     9781 atacattata aaaaatctag ggggataatg aaatgttaaa cgaacaaaga aaacaagaag
     9841 taactgaaat atgccaaaaa ttaattcaaa atcctagttc ttctggaaat gaagaaggtg
     9901 ttgtaaaagc aatagaagag tcttttgaaa aattaggttt tgatagttgg tcaagagata
     9961 gatatggaaa tatagtagga tgtataaaag gtaacaagcc aggtaagaaa atcttatttg
    10021 atggacatat agatacagtt ccagttccag atgcaagtaa atggagtgtt cctccttttg
    10081 aaggaaaaat agttgatgga aagatatatg gtagaggaac ttctgacatg aaaggtcaat
    10141 atactgcaat gatgtctgct gtagcgtatt ttgctaaaga cactaataga gattttgctg
    10201 gagaactata tgtagcaggt gttgttcatg aagaaatatt tgaaggtgtt tcagctagag
    10261 agataagtaa ggctgttcaa cctgactatg ttgtaatagg tgagtcttca gagttaaact
    10321 taaagattgg tcagagaggt agaggagaaa tagtagtaga aacttttggt aaaccagctc
    10381 actctgctaa tccagaaaaa ggtgtaaatg cagtttataa aatggctagt gtaatccaaa
    10441 agatacaaca attagtacct ccaactcatc cagttttagg tgatggaata ttagttttaa
    10501 cagatataaa atcttctcca tatccaggag catcagtagt gccagattat tgcaaagcta
    10561 cttttgatag aagattatta gttggagaaa caagagaagg cgtattagct ccaatacaag
    10621 ctttattaga tgaaatgatg aaagaagacc cagaattaaa tgcaaaagtt tcttatgcgg
    10681 ttgaaaaagc tgattgctat acaggaaata ctatagaaag tgaaagattc ttcccaggtt
    10741 ggttatatga cgaagaagat gaatttgttc aagcagcata taagggatta aaagaagctg
    10801 gaatagattc agaaataact caatactcat tctgtacgaa tggaagtcac tatgcaggtg
    10861 aagctggaat aaaaactata ggatttggtc cttctaagga aaatcttgct cacacaatag
    10921 atgagtatat agagcaagaa cagttatttg taggaacaga aggttactat ggaatattaa
    10981 aatctgtata cggaaaataa tatttagttc acaataaatt atttaaatat agtctagagg
    11041 tgctgaatat agcaccttta gaaaatacaa ttttaaataa agttttacaa tacttagaaa
    11101 atactttaaa gcttaaaagt taggaggtat atatgggaac aatactaaga ggtggaacta
    11161 taattacctc tgataagact tatataagtg atttaaggat agaagatgaa aagatagttg
    11221 aaataggaaa taacttagaa ataaatggtg ataaggttat tgatgctaca ggtaaaattg
    11281 taattcctgg tggaatagac acacatactc attttgatat gaatgcaggt tctattacaa
    11341 ctgcagataa ttttgaaaca ggaacaagag cagctatagc tggaggaact actacaatac
    11401 tagattttgc agaagccaat gagggggaaa acctactgca aggtgtagaa gcatatcata
    11461 aaaaagcaag tggaaattgt tattgtgatt atggttttca tatgacaatt acatgtttaa
    11521 ataaagatac atttaatcat atggaaaaac ttatttcaga tgggatagta tcttttaaga
    11581 tgtatatggc atatgatggc atgaaagtag atgatggaac aatatacagg gttttaaaga
    11641 aagcaaggga tttaggttgt atagttggat ttcattgtga aaatggggat ttactagatg
    11701 tattgataga agaaaatata caaaatggta atttagagcc aaaatatcat ccacttacaa
    11761 gacctaacat agttgagaaa gagtctgttt caagacttgc tgatataact aaattatcta
    11821 attcaaaatc ctatgtagtc cacttaagtt gtagggagtc acttgaaact gtaaaaatgg
    11881 ctagagaaaa aaatatagat atgatagtag aaacatgccc acagtatcta ttattagaag
    11941 acaatttata taataaggat aaatttgagg gtgctaagta tgtaatgtct cctcctttaa
    12001 gaaaaaaaga agacataaat tacctttggg aaggattgtc tagtggagat attcaaacag
    12061 ttggcacaga ccactgttca tttaatttta aggggcaaaa agacttggga attgatgatt
    12121 tcagtaagat accaaatgga gcacctggtg ttgaacatag gttagcactt ttatatacat
    12181 atggggtctt agaaaataga ataagtgtaa ataagtttgt ggaagttact tctacgaatg
    12241 ctgcaaaaat atttggaatg tatcctaaaa aaggtgagat agctgtagga agtgatgcag
    12301 atatagtcat tttaaatatc aataaagaag aaacaattag ttataaaact caaaaacaaa
    12361 atgtagatta tactccatat gaaggattta aggttaaatg taaagttgaa gatgtatttt
    12421 tgagaggaaa tcatgtagta caaagttgta atgttaaaga acatccaaca ggtcaatata
    12481 ttaaaagaaa aattactaaa taatgcgggg ggaaataaga atgatggata tacttattaa
    12541 gaatgccata atagtaactg tgaataaaga gagagaagtt atttttgatg gggcattagt
    12601 agtaaaagat aacaagattg ctgatattgg aaattctaaa gagatagaaa gtaaatatac
    12661 tgatgttaaa aaaattatag atgctaaagg taaagtttta ttcccaggtt ttataaatac
    12721 acataatcat ttattccaaa ctttattaaa aggattggga gatgatatgg tacttaaaga
    12781 ctggttagaa actatgacat ttccagcagc taactattta gagccaaaag atacttatga
    12841 tgctgcaatg cttggatgta ttgaaggact tagaagtggt ataacaacaa tggttgacta
    12901 catgtatcct catagtaagc caggtctttg tgatggaata atagatgctt ataaagaatt
    12961 aggaataaga ggaatacttg gaagaggatg tatgaataca ggagctcaat ttggagttca
    13021 tcctggaata atgcaagatg tagaaacggt tgaaaaagat gtaagaagat tgtttgaaaa
    13081 acaccataat acagaaaatg gaagaatcaa aataggagta gctcctgcag caatatggtc
    13141 aaattctcaa gaaatgcttg aaatgctatg gagagtagta aaagaatatg atgatgcttt
    13201 atttacagta catatatcag agactccttt tgatagagaa gcagcaaaag aattgcatgg
    13261 gcaatatgac atagatgttt tagaaaaatt aggaatactt ggaccaaatg tgcttatggt
    13321 acattgtgtg tatctaactg aaaaagatat ggaacttact aaaaaatacg atatgaaagt
    13381 gtctcataat acagcaagta atatgtactt atcttcaggt gttgcaccag taccagaaat
    13441 gcttaaaaaa ggaataacag ttagtttagg agtagatgga gccgctagta ataactctca
    13501 agatatgctt gaacttatga aattaacagc acttcaacat aaagttaata agtgtgaccc
    13561 attagcaatg tcagcagaaa aagttcttga gttggcaact atagatggag caagagctat
    13621 tggaatggaa gatgagatag gttcattgga gataggtaaa aaagcagact tactaatatt
    13681 taatccaatg ctttcaccaa aagcgatacc tatgcataat ccggtatcta cattagtata
    13741 ttcttctagt atgaaaaata tagagagtgt tatagtagat ggaaatataa taatggaaga
    13801 ttcaaaaata ttgacagcta atgaagagaa agcattaaaa gatgctcaag atacagctga
    13861 gagactttgt gtaagaggaa ctataaaaaa tagaatggaa ggtcataaat ggaatagcct
    13921 ttattaataa atgtaattta ttcatagaaa agtatagtaa aactttagct taaaaatatg
    13981 agttttgagg tgatttattt gattatcaaa tgtaccataa ataataaaga aaaagaatta
    14041 agtataagac cagatgattt tttagcagat acattaagag aaaatggata tgtaagtgtt
    14101 aaaaaagggt gtgatacagg ctcatgtggg ttatgtacag tgatggtaaa tgacaaggcc
    14161 gtgctttcat gtaattatct tactgctaga gtagacggtc agcatattac aacacttgag
    14221 ggtgtcttgg atgaagcaaa gaagtttgga gattttcttg cagacgaagg ggctgaccag
    14281 tgtggatttt gtgctcctgg atttataatg atggttttgg caatgaaaag agagcttcaa
    14341 aatccaacag aagaagaagt tatagattat gtaaatggta acctttgtag atgtacagga
    14401 tataaaggtc aattaagagc aatacataaa tatttaaaca cttagacttt taagtgaaaa
    14461 ttaagttgat atggggagag agtatgataa ctataaagga atatgtagtt ccaaaaagtt
    14521 tagatgaagc ttatgaactg ctaatatcaa gaaaaaacaa cataatactt ggaggatgtg
    14581 gatttttaaa actaggtagt aaaaatattg gctcagcaat agatttaaaa gacttagctt
    14641 tagattatat aaatgaaact gatgatagca tattaattgg agcagacaca agtttaagaa
    14701 ctttagaatt aaataaggtt attaaaaatt actgtaatgg ggtaatttca aacgctgttt
    14761 ctaatatagt aggagttcaa tttagaagtg gagctagagt aggagctagc gtatttgcta
    14821 agtatggatt ttcagattta ataccttcat tactagtggt agatgcaaag gttaagttat
    14881 ataaaaaagg ggttatgaac ttagctgatt ttttagaaag tgagcttgaa aaagatatat
    14941 taatagaagt tatattacct aaaaaagatg ctataggtgt atttgattct ataagaaaat
    15001 gtacaggtga ttttgcagta ttaaatggag caatgttaaa agaaggaagt atttataaaa
    15061 ttgcaatagg cgctagacca agaagagcaa aaattgccta taaagcaagt gaaatattaa
    15121 gtttagaaaa tgatatagaa aaagctggtg aagtatcaag tgaagaactt tcatttggaa
    15181 gtaatataag aggaagtaaa gaatacagaa aagatatggc aaaagcctta gtagtgagaa
    15241 tgtataatat tattggaggt gagtgtgatg gtaaataaag aggtaagaaa aatagatagt
    15301 gaagccattg ttactggaaa accaatatat acagaagatt taatttttca taaagatgtt
    15361 ttaacaatta agcttcttag aagtccacat gcttttgcga agataaaaaa catagatata
    15421 aaaaatgcat taaaggtaaa aggtgtagta aacatattca cttacaaaga tgttccaaat
    15481 tatagatatt caatggttgg agagtcatat ccagaagcat cacctcatga ccaattaata
    15541 cttgaagacg tagttagata tgtaggagat gaagttgcta taatagccgc tgaagatgaa
    15601 agggctgcta ttaaagctat gaaacttata aaagttgaat atgaagtaat gactcctata
    15661 ttggatgcaa gagaatcgga aggaaataaa attttaatcc atgaagaaga tgatttgttt
    15721 tgtccatttg attttggatt ggatgctaca aaaaatattg tatcgagaga aaaaatgggt
    15781 aaaggggatg tagaaaaaga gtttaaagaa agtgatgtaa tagtagaaca ttcttatatg
    15841 actcaatcac aagcccattg tatgatggaa actcatagag cttatacgta tatagatacg
    15901 aatggcagat tagtagtaac ttctgcaaac caatcagctt atcatatgag aagacaggtt
    15961 tctaaagcac tgggaatacc tctttcaaaa gtgagagtaa taaaaccaag aataggtgga
    16021 ggttttggtg gaaaaaacat agctgtaaca gagatatatg catcatttgt aacttggatg
    16081 acaaaaagac cagcaaaact aatttatact agagaagaaa cttttgcaat gactaatact
    16141 agacatcaga tgttttttga tataaaaatt ggggcagata aagaaggaaa cataaaagct
    16201 atagatatga aagcattgaa caatacaggc gcttatggtg ataatggacc ttcagtttgc
    16261 tctgaatcag gtcataatgt acttccaaca tataataatg tacctgcaat aaaatttgat
    16321 gggagaactg tatatactaa tttagttcca ggaggagcat taagaggata tggagcaaca
    16381 caaggtacat ttgcacttga ttgtgcaatt gatgaattag cgcataaact taatatggac
    16441 ccaacagaaa taaagttaaa gaatattata aaaaaacaaa ctgaaggcgg aataatgaat
    16501 tttccaataa gaagttgtgc acttgatgaa tgtataatta gaggtaaaaa acttattgga
    16561 tgggatgaaa agtaccctgt tagggatatt ggcaacaata aggtgagggc tgttggaatg
    16621 tctgtgggaa ctcatggttc tggtatagcc aacatagaca tggcaatagt aaatatgagg
    16681 atggatgaag atggaactta taagttgttt tctgggtctt cagacttagg aactggttct
    16741 gacacaatac ttgcacaaat agctgcaaaa gcattgaata catctattga tagaatttcc
    16801 gtttatacag gagatacaga tatgtgtcca tatgatactg gtgcatacgc ttcttgtact
    16861 acttatgtaa ctggaaatgc tacaataaaa tctgctgaaa gtttaaaaca aaagatatta
    16921 aaagtagcac agaagaaact cgatttacca atagatgatt taatactata tgaagacaga
    16981 gtttgttata gtgaagatgt agagaagttt gttttgttaa gtgaattagg aaaagaatct
    17041 gttggtggtg gaaatcaaga agttttatat tcatctgaat cttatggtat acaagagagt
    17101 gctaaaccat tcttagcagg atttgctgaa atagagttag ataaaacaac aggggagttt
    17161 aaagtaataa attatgcttg ttctacagat tgtggtaccg ttatgaatcc tgcacttgct
    17221 agaatacaag ttgaaggtgg tgtaactcaa ggtatagggc ttgcaatgta tgaagaccct
    17281 agacatggtt ctgatggaaa gcttcaaact tctaatctat tacaatacaa agttccaaca
    17341 aaaaaagata taggaaacat aattgttgat tttgtagata attatgagcc aacaggtcca
    17401 tatggagcta aatctttagg agagatagtt attcatacac ctgcaccagc aatagcaaat
    17461 gcaatataca atgccacagg agttcgtata agagaacttc ctataacttc tgagaaagtt
    17521 tatatggcca tgaaaaaatt aaatcaggga ggtaaataaa tgattgctat taaaagtaaa
    17581 tacttaatat cttctgctga tgaaatatat acaaattctg ctgttgtagt agaaaatgac
    17641 attataaaag atatattgcc aaatgaagaa gttgaaataa aatataaaaa tattgaagaa
    17701 ataatagata aatcagatgc tataataatg ccaggattcg tcaatggtca tatgcatcaa
    17761 tatggagttc tttctagggg aataccggca aatgtacatt ttactgactt tgaaggtttt
    17821 ttaaatgact attggtggcc atttatagaa aatagaattg gattaaaaga agtgaaggca
    17881 acaactaaag catcagcaat agaacttata gaatctggtg tggtagcttt ttgtgatacc
    17941 ctagaagctc caaatacaga agaaggaact ttaattgagc aaggaaaaat actagaagaa
    18001 ataggcatga aagcgatttt atccttagaa agttgtgaaa gaataagctt tgaaaatgga
    18061 cttagatgtt tagatgaaaa ttctaattta ataaagtgga gtagagaaaa tagtaaatta
    18121 ataaatggaa taatgtgtac acatacaagc ttcacatgtt ctgatagatt tattaagaaa
    18181 gctaaagaag atgcaaaaaa attaaatgca ccaatgcaat ttcatttatg tgagagtata
    18241 tatgagccaa actatgctga aaaacacttt ggcaaaaaag cagttgacta ctataatgat
    18301 ttagatatat tggatgaaac tgtattagca tctcagtgtg taaaagtaaa tgatgaagaa
    18361 atagatatat taaaagaaaa aggcgtaaaa gtggttcata tgcctcttag taactgtgaa
    18421 gtaggtggag gtttttctcc agtaccaaaa atgattaaaa aaggcataaa agtggcactg
    18481 ggtactgatg gatatataaa tgattttttt gaagttatga gaggtgcatt tttaatgcat
    18541 aaatcagttg aagaagatgc atcagtaatg ccagcaaatt tagtatttag aatggcaaca
    18601 gagcatggtg cttatgtatt aggacttcaa aattctggta aaatagcagt tggtaataag
    18661 gctgatataa tagttatgga agatgaattt aaaactcctg tgactcttga taatatattt
    18721 gaccagatag tagttcaggg taaaaaagaa tttatatcaa atgtatacat agatggtaga
    18781 catattttaa aagaaaaaca gttagttgat ttagataaaa aagctattgt taaagaaatg
    18841 aaagaagttg cttgtgaatt ttggaaattt taacgaatgt aaatatatat gaaaagggga
    18901 agcagataat gtataatttt ttaggtaaag aattaaaatc accacttata ataggttcag
    18961 gaccattaac atatagtgca gctggatgca agatattaag tgatgcagga gcaggggcag
    19021 ttgtgactaa gactataaga aaagaaagag ctataaatcc agcaccacat atggttagaa
    19081 atacagctaa tgctctttta aataatgaaa aatggacaga ttttgagcca gagcaatgga
    19141 tagattttga aataccacaa atgaaaagag atgggactgt ctgtatagct tcaataggtc
    19201 atacaattga agaaagtagc gaattagtag agaaagttgc aaatgcagga gctgacttta
    19261 ttgaattagt atcttatgac tatagagatt tgataccaat gcttaaagat gcaaaggaaa
    19321 gagtaaatat acctgtaata gttaaacttc ctcctatgat agatgagata ggcgattttg
    19381 ctaaaaaatt agaagaagca ggagcagatg caataactgc ttgtgactct gtaggaccag
    19441 catttagaat agacatagaa acagggcaac ctttattagg tggaaatgga ataggatatt
    19501 taagtggaga aactatcaag ccaataactt tacagagaat atatgaaata aaaaaacaag
    19561 ttaatatacc aataattggc ttaggtggat gtgtaagtgg agatgatgcc ttagaaatga
    19621 ttatggcagg tgctgacttt gttggtatat gttcagtagt aattttaaaa ggcgcacaag
    19681 taatatctaa aatacatgat gatttaaaat ctaatcttaa tagattagga tataatacta
    19741 tagaaaatgc ttgtggtata gtacataatc atatgggaga tgttaatgaa agacttagct
    19801 ttgagtttac ttatgatgaa gaaaaatgta ctaaatgtaa gatgtgcgag agagtttgtg
    19861 catatcaagc gagaaaattg aatgataaga tggaattgaa tgaagaagag tgtagatatt
    19921 gtggactttg tatatcagtt tgtaaaccaa aagcacttgg aagaaaaatt tcatgttcag
    19981 agctgaatgt atagattatt ttataaaaaa caaagtttta aataaaaata agaaaggcta
    20041 tctcaaagag ataagccttt tttgttgttg accttatttt tgtttatttg tagttatttt
    20101 agatattaat tagttttgaa gtaataaagt gaatcaattt tgtttaatta aaattttaat
    20161 aattctgata tatatgttaa cattgtatag catttgtgta aaatgttata ataattaata
    20221 gggatggtga tgcaagtgca agagaagatt ttagagttat tatatactat aaatgaagat
    20281 gtagttagca taaaaaatga tatgtcggaa gtaaaacaag aaataaatgt gttaaaagaa
    20341 gatatgtcgg aagtaaaaca agaaataact gtaatgaaag aagatatgtc ggaagtaaaa
    20401 caggaaataa atgtattaaa aggaaatatg tcagaagtaa aacaggaaat gattgtaatg
    20461 aaagaagata tgtccgaggt aagacaagag ataaatataa tgaaagagga tatgtccgag
    20521 gtaagacaag agataaatat aatgaaagag gatatatccg aagtaagaca agaaatgact
    20581 gtaatgaaag aagatatgtc ggaagtaaaa caggagataa atgtattaaa agaagatatg
    20641 tccgaagtaa gacaagagat aaatataatg aaagaagata tgtctgaagt aagacaagaa
    20701 atgactgtaa tgaaagaaga tacatcagag gtaaaacaag agataaatgt aatgaaaaaa
    20761 gacatgtcag aagtaagaca agaaataact gtaatgaaag aagatgtttc cgaagttaaa
    20821 tcagaagtaa actttatgca aaataaaata aataacatta atgaagatat gaatggaatt
    20881 aaggacgagg tatccatagc aaatgaaaaa ttatatggaa ttgaaattaa gattgatagt
    20941 ctagaatgtg aagataaatc aatgaaagag atacagatag aacagaataa tattttagga
    21001 agtttattac ataattctga aataaataaa gctactcatg ataatataga acataacata
    21061 gcatatataa aaggtgacac taattctata aaagaggata tagctgaaat aagaagagac
    21121 ttaaatttag tagagttagc cactagtaaa aattggagtg atattgtcaa actaaaatca
    21181 gtaaagtagt attttaattc actaatttat catccacaaa ctaagaattt tatataaaag
    21241 aatttatagt gt
//
LOCUS       DAGSWI010000065        20793 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.065, whole genome shotgun sequence.
ACCESSION   DAGSWI010000065 DAGSWI010000000
VERSION     DAGSWI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.065"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(196..732)
                     /locus_tag="MJF80_002219"
     CDS             complement(196..732)
                     /locus_tag="MJF80_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBZ0280877.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFDSDIEEEN"
     gene            complement(750..2063)
                     /locus_tag="MJF80_002220"
     CDS             complement(750..2063)
                     /locus_tag="MJF80_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBZ0280878.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2418..2858
                     /locus_tag="MJF80_002221"
     CDS             2418..2858
                     /locus_tag="MJF80_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBZ0280879.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(3043..3231)
                     /locus_tag="MJF80_002222"
     CDS             complement(3043..3231)
                     /locus_tag="MJF80_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBZ0280880.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
     gene            4264..4659
                     /locus_tag="MJF80_002223"
     CDS             4264..4659
                     /locus_tag="MJF80_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280881.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4702..6801
                     /locus_tag="MJF80_002224"
     CDS             4702..6801
                     /locus_tag="MJF80_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBZ0280882.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNTSTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(7026..12236)
                     /locus_tag="MJF80_002225"
     CDS             complement(7026..12236)
                     /locus_tag="MJF80_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBZ0280883.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSMDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12251..14746)
                     /locus_tag="MJF80_002226"
     CDS             complement(12251..14746)
                     /locus_tag="MJF80_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0280884.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIER
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15383..15670)
                     /locus_tag="MJF80_002227"
     CDS             complement(15383..15670)
                     /locus_tag="MJF80_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280885.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15690..15965)
                     /locus_tag="MJF80_002228"
     CDS             complement(15690..15965)
                     /locus_tag="MJF80_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280886.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16600..16779)
                     /locus_tag="MJF80_002229"
     CDS             complement(16600..16779)
                     /locus_tag="MJF80_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280887.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(17064..18530)
                     /locus_tag="MJF80_002230"
     CDS             complement(17064..18530)
                     /locus_tag="MJF80_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0280888.1"
                     /translation="MKRKPQYLVIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLLTYM
                     YKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIGDCL
                     PSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYITLFSEYDDDYINTEEGAK
                     RLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDFVLL
                     CKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYLKKG
                     DVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
     gene            19512..20690
                     /locus_tag="MJF80_002231"
     CDS             19512..20690
                     /locus_tag="MJF80_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBZ0280889.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFGNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
BASE COUNT         7054 a         3537 c         2258 g         7944 t
ORIGIN      
        1 aataaattca tattaaaatg cattcagtta tataattttg tttatcctaa gaatataatt
       61 ataaaaataa aaaaagaata ggataatttt cctactcttt tttctgtact tacatataat
      121 attaaaactc tttatttttt aatatatatt ttaaattttc ttttttaata aatctttttc
      181 ttttaataaa tctctttaat tttcttcttc tatatcagaa tcaaagggaa tccatgcatt
      241 accaaactct ttatctttaa ataaataagc taatccagtt atttgcttca atctaagaat
      301 ttcatcaact tccataccca ggtgctttga tatccattca tcagattttc caatttcaca
      361 aagttcagtt ataattttac tcattaaatc cacattatga gaacctcttg cacgattgtg
      421 tcgaatagta cttgccattc tttcatcaag agatttatca attactgaaa caggaagaag
      481 tccattttct ctatcatata tatcctcatt ttcaagcatt actctatatc tatgaaaacc
      541 atctacaata atatacttat ccttttcctc attataataa cagacaattg gcattgtata
      601 cccatcttct tttatacttt tatacaacaa ctgcatttct ggtggggcta ctgcatttgg
      661 attatagtcg ttagattcta tcttttcgat tggcactgcg atgattccat atacaggact
      721 tttatattcc atagcatctt aactcctatc taatgaattt tttcatattt cttcttaata
      781 tattccgttc taattctctg ttcttttgta ggaccaaatc ccataaatct acacatatgg
      841 tcatttttta aaatacaata acacattctt ttccaacttg gtatatcttt ggtaaattta
      901 atatcatccg tatcatcagg tatatcatcc aaaaatatga ctctactctt tttatctttt
      961 gtatgatttg aaactccatt taattctatt ttataacctt ttctcaacaa ttcacgtata
     1021 actatttctg acaaaccacc acctgtattc tgccaaaact caatagatgt tgaaaatttt
     1081 ttaatatagc tattccttat attttcaggt aaggtatcaa gcaaaaaata tgtgaaactt
     1141 ttccatgtgt gtccatatgg caatttaaca ctacgatatc ccatagcttt tgttttgcca
     1201 taaatagaag taaaattaac tccttgtact ctagcaacca atttcaccca tacatctggg
     1261 tccaatattc tgtataaatt taaactctct tttgctgaat cattaaaagg tgatgccact
     1321 ctcatttttc ttggagctac tccagcttta tagaataagt cataaatttt attatagtca
     1381 tagtcaaatt tatagtgtgc taaccatata tcttctattt cccaatcata tataggagat
     1441 gccgtccaga catttttaaa ttccttagtt atccagcact ttcctttata tccatgctgt
     1501 ttattcacaa tagaacaata acgttgtaga gattctgctg ctcgtgttcc taataagcaa
     1561 acaacttttg caccttcatg tatatctcta atccatctac caaattgttt tgccaaactt
     1621 tcttgtctca ttttatattt ataataagta aaaggattat ttttttgatt tattatgtat
     1681 tctttgactg gcatttccct tacccataac tcttctttct catcatccca tggataccaa
     1741 tacatttcat aatttcctac agcagttctt gtagccattg gtaaacacac ccaatatggt
     1801 tcaatatctt caatattatc ttcaaatact ttttcaacat attcagtagt caaactatat
     1861 tgagcctcaa aatcttgatg aaatactcct attttggaac ttatattatt ctttctctta
     1921 taatccaaaa ctaaatttag cagtaaacca ctatcttttc ctccgctaaa agatacatag
     1981 atataatcaa attcattaaa aagataagaa agtctttctt gaaaagcttc aaatacatcg
     2041 gattctaaat atctcttatc catttgaatt atcccctttc aattagaagt ttataaaacc
     2101 ttacaatgca atattattat gcttttttag cagtaatcag cataaattat acattattta
     2161 aatacacaaa tccaactaaa tacatataat tataatgtaa ttatactttt gtattataaa
     2221 ctatatttta tcattatttt acattttttt ccaatatata taattatcat atttttctat
     2281 tagctaaaac taattacctt atatttttac gtattggtat aataatacat aaaaacttga
     2341 ataatgcata aaaacttgac atactgttgt cataattact ttgctataat taaaagtaaa
     2401 aatggaggta aattctaatg tatgaacgta tgcttgataa aagcaataaa ccctgtttgt
     2461 ctcaaataat agaatatata ggtaaagatg gatatgcacg attgtgtaag ttggaaactt
     2521 acttgaaaac tcaatataat ttatcaagag aacttcgatt tccttttgga aacaattatg
     2581 gttggggtta caaatatagc cataaatctt ttcatttatt ttatgctttt tttgaaaaag
     2641 gtgcatttac tgttctgatt cagataggtg acaagcaagt tccagcagta gaaaaaacgt
     2701 tggtagcttt gacaccaaaa gctaaagagc tctgggtcac tcgttatcct tgtggcaata
     2761 atggaggttg gattaagtat cgtattttag aagatgcaga cttacttgat attattcaat
     2821 atataagagc aaaaaaatct ccacctaaac aaaattaact tttatttttt gtgatttaaa
     2881 tatagtgtat ataaattcca ttctttccat ataaaaaaaa taatataaaa aatatctatt
     2941 agtataactt atttagtatg tcttattcta ctttttacag ataattaaaa agttttatca
     3001 taaataaatc ataactagac atcctagtaa atcaaaatta atttattctt ttttattcga
     3061 atttttgata atagtcccta tacatatgcc aattatcatt ccaaaaatta gaccataaca
     3121 cattgtgata cctacatttc ctttagaaat acctatagca caacctatag ccattccaag
     3181 tatcatgcct ataaacatac atataataac taaattgttg tttttattca tagttttctc
     3241 cattcaatat attttaaatt gcctagctcg ccttcttagc ttaaaacaaa gaaatataaa
     3301 ttaactttct ttcattctaa tatctgcctg tataaatgtc aaacaacaca tttcttgtca
     3361 taacctcctc tttttgaagt aaatataatc tcccagacgc aataatatca atttcctaaa
     3421 accatttaag taacttttat atttacataa tatttacaat cataaactat attgttattc
     3481 atttatacat cataaacact ccctcctaat cattattata cttaaaataa tgttcattta
     3541 taataatata tctaacgcac tttaattgaa aaaactaaaa ctttgccttt cattcccata
     3601 tataaataag tatacttttt gatgctctat tacaaatcaa ctgaattaat tgatataaat
     3661 tatcattttc tgttgaaaat accattcgca catgctatac ttaattgcat aaatggtaat
     3721 aaatgtcata tgattttaca aattattaat aacaattatt acttttactg tattttaaat
     3781 tttggggact aaatatcaag aactttctaa aatacaaagt acaaaaaatt tatataagtt
     3841 ataaatttat atgaactaga taagctcaaa caatatgtta tgtataaact gtgtagtatt
     3901 cggttataaa tttatataaa ttatataagc tagataaagt attacctcaa ctatataagc
     3961 tagatatata aattgcttat ataagctaga taagaattat ttcaactata taaactacat
     4021 atataaatta catacataag ctagatatat aaactagata tataaactgc atatataagc
     4081 tatataagaa ttatttcaac tatataaact acacatgtaa aattcatgta aatttactaa
     4141 tataaatcaa ataaaattac taaactctct aaactaattt tgtaaaacat actctatttt
     4201 taacatttgt taccttatac ataagtacaa acaaaatcta actaaatgaa aggataatgt
     4261 aaaatgaata ttttaaaaat gaaaaaaaca gggatatttt taggaggagt actttttggt
     4321 actgtaggtg ttaagatact ttcaagtgat tgtgttaaaa aaacttgcac tcgtgcaaca
     4381 gcaggagcat tgaaagttaa agattctgtt cttgaaacaa ctacgaaaat tcaagaaaat
     4441 gtagaagata ttttagctga agcaaaagaa atcaacgagc aagaagactt atctacatct
     4501 aaggtaaacg ttgtacaaga tactcttaaa tgcgaacaag aagttcttaa aactgaaact
     4561 ataaaaactg tagaagatac taattctaat agcattaaat gtgaagaaat caaagataac
     4621 gtaattgaag aaaaaaatag tacttgtaca acagaataga tttaatgatt attatgatta
     4681 ataatatcag gaggaagtaa attgaatttt tatataaaac acgaaataag aggtcgtata
     4741 cgctttgact tacaacttgg caaattaaac aacaagcagt ccgatactct cttatattat
     4801 ttaacttcta taaatggtat taccaaagca aaagtttacg atagaactgg tgatgcagtc
     4861 atttattaca ctggtgaaag agaatacata ataagagaaa tcactaaatt cagttttaac
     4921 aatagcaaaa tagaaaacct agttcctgaa cataccagca gggaattaaa caattattat
     4981 agagacaaat tagcaaataa actgctagtc aaaagtatct ccaaaatatt ttttccaaag
     5041 tccttaagat tttttttaat tggacttcat tctataaaat attttaaaaa aggtctctct
     5101 tccctgctaa ataaaaaaat agaagtttct gttttagatg ccactgctat aggtatttct
     5161 atttttagaa aagatatcaa tacggctggt tcagttatgt ttcttcttgg tatcggtgaa
     5221 ctattagaag aatggactag aaaaaaatct atagacgact tggctcaaag tatgtcacta
     5281 aacattgaaa aagtatggtt aagaaaaggt gacactgaaa tcttaattcc aatttcagag
     5341 ataaaggaag gagatttagt aagtgtaact atgggaaata tgattccatt ggatggagtt
     5401 atagtatcag gagaaacaat ggtcaatcag gcatcactta caggtgaatc ccttgcagtt
     5461 aacaaaaaag aaggctctta catatatgct ggaactgtaa tagagcaagg caatattgtt
     5521 atgtgcgtca aggaaaaagc tggtactact agattccaga aaatagtaac tatgattgag
     5581 gaatctgaaa aactaaaatc ttctgtagag agtaaatttg aacatctagc agatacatta
     5641 gttccataca gctttttagg aagtatatta acctatgcaa ttactagaaa ccctataaaa
     5701 tcactctcta tattaatggt agatttctct tgtgcattaa aactctctat ccctatttca
     5761 gtactatcag ccatgagaga atgtaacaat aacaacatta ctgtaaaagg tggaaaattc
     5821 cttgaaggtg ttgcatgtgc agatactatt gtatttgata agacaggcac attaacaaaa
     5881 gctcaaccaa ctgtttcaga tataattaca ttccaaaatt ataataagga ggatatgtta
     5941 agacttgcag cttgcttaga agaacacttt ccacactcca tagcaaatgc agtagtttac
     6001 gaagctgaaa aacaagggct tagtcacaaa gaaatgcata cagaggtaga atatattatt
     6061 gctcatggca tttcaacaaa agttgatgat aaaaaggttg taataggtag caaacatttt
     6121 gtatttgaag atgagaagtg tactatccca gacggtgagg aggataagta caacaatcta
     6181 tcagatgagt attcacacct atttatggca atatcaggta aattatcagc agttatttgc
     6241 ataaatgacc ctttacgaaa agaagcaaaa tatgtaattt caaatttaag agaatgcgga
     6301 ataaaaaaga ttgtgatgat gactggagat agcgaaaaaa cagctaaatc tatagcaagt
     6361 aaagttggtg ttgatgaata ttattcagaa gtcttaccag aagacaaagc caattttgtt
     6421 aaaagtgaaa aactcaaagg tcgcaaagta attatgattg gtgatggaat caatgattct
     6481 cctgctattt cagaatcaga tgtcggaatt gcaatgagtg aaggtgctga aatagcaaga
     6541 gagatttcag atattaccat atcagcagat aatttgaata atttaatcgt tttaaagcag
     6601 attagtaata agcttatgaa aagagttgat ttaagttcta agtttattat tggctttaat
     6661 ttaggtctga ttttacttgg tgtaggtgga tttgttagac caagtacatc tgccttttta
     6721 cacaacacat ctactgtcgg aataagttta aacagtatga caaatttatt ggaaaacacc
     6781 aatacttata actaccatta atctccatta cataaaacat atgatataaa aaatttataa
     6841 ttcatctttt taaaagaata tgtcttaaaa ttagcttttt tctaatcaaa attatataaa
     6901 gcatacttta caaattttaa actcaactta tttctatttt ttacaaaaaa ggagatgatt
     6961 caaattttca ttttgaaaca tccccttttg taaatttctt taaaatttat atattaatta
     7021 tattttcaac caataattgt attttcaacc aatctttctt catagcattt taaatcattt
     7081 tgctttagat aattaaattc agactctaca taaataccac agcctaccaa ttcaattgtc
     7141 aaattaggta tattttcctt tagtatacta gtctgctcat aaataattct ttccaataaa
     7201 tcaacttgta atatagaaaa atgcttcata atctccccat cactatctat atctttcttg
     7261 atttcatacc aactctttga caaaattccc ttcaaatcaa tataccctag actcctcaat
     7321 atatctatac tatcgctcca tgcatactta ctaggtttat caaagtatga ttctagtttc
     7381 ttcttctgct catttgtaag tgaatcataa gaaagcttct tattaaaatg tgccttcaaa
     7441 gaaatcttag ccatagaata tggacttcta ccagagttta atcctgcatt tttgaacttt
     7501 tcaattcctt tatcttctac tgaagaccat attgagttta ttgaatctga atcattgtat
     7561 ttaatatcaa gaccatgtgt aatatttata tagttttgtg gaaatacata ttgataatag
     7621 acatcttttt tatcatcata tagagagaag tatccactat catataaatg aatatgcacc
     7681 tttactttta aactatcctc taaaatattt cgtctaaggt tcgggatatt tgcatacatg
     7741 gttacatatt ttatggcatc aaagttttta tttaatggaa cttcaaaact aatttcatct
     7801 cctgtcaaaa gctttttcac ttcttcttca ttacaatcat atagagaata tgtattttca
     7861 aatcccacac gtgatttccc atctaaactg cctgttaatg ctaccttcaa aatatggtta
     7921 tctgtttttt gccattggta agcatcttgg tcaagaaact ctctaatctg tctattttgc
     7981 tctttcgcct gatttataac ctcgttttca tcttctgtaa tccctacatg gcatctattt
     8041 aagatattta ctatattgct tccaaatggg aatgtccata gttgaaaagc tgctgcctca
     8101 acctgacaca aatatctaac ctgatttaat tcaatttgat ggataagttt tttctgatat
     8161 tcattcatct gattaccact atctgacatt tgataaatca attctcccat tgcaagatta
     8221 tcttttttct tatcaatctt atcagtaatg ataatttgag tttccatcat atcctgtact
     8281 tttttaaata ttatagaaat ctcctctgag ttttccattt ttaagcttcc aaacttcgtg
     8341 cttaattcag ttaaaacttc cttcatcttt aatttactta tctcattttc aaaaatatct
     8401 tgatttttaa ctgtactatc gaatttttct acaaatggta taacagattc tttcaaagtt
     8461 ttatctttaa attccaccat ttgagtattc aattcatcta atttcttaat attattttct
     8521 ttatctttaa ttgcctcttt aactacctct attttactct caaaactaga ttttacatta
     8581 agacaattat taacaccact aatcgcagta tttataagag aacaagcaga acttccaata
     8641 ctcaatgcat ttttaatttt agatgtcttt tgctgtcttt caatagcttg tttacttttc
     8701 tctagtgcag ctaacttatc tttttgctgt ttattattgt aatttgcctc caacatacct
     8761 atttcttttt tctggatttt tgtgtaaatt tccttatact cgctttcact taacccctct
     8821 tttgctcttt gaatatcgtc agctttgtct gcatagttat catcatctat agacttaact
     8881 ataatctcct taatttggtc aaatttagtt cttctttctt catcaacacc tgccaaatta
     8941 taagtcattt tatctaaagc attttttcta tcttcactta gctgttctcc aagtacaaat
     9001 ctcataccat cagaatccaa tttaaaatct gaaaaatcaa ccatcctgtt agtccattgg
     9061 tattcctctt ctttagctga aagattttcc ttcaaagatt ttgcactgtc aaatgcccct
     9121 agacaagctt gaccaacacc actaaatgca gtcagtaaac tcaatgcgct ttgtattcca
     9181 gaaagaatta actgtgtttt catttgttcc tcaagttttg cctgtttctt ttttagttcc
     9241 tctatgtctt cttgttcatc ttctttcatc tttataattt cttgaatcaa caggttcatt
     9301 tttgcattaa cagaaagcat tttattctca agttcatttt ttaaaacctc tatttgttga
     9361 tttccagatt caatttgagt taggatactt tccttatatg tattgaaata tccttctttt
     9421 aactcatctt tctttccttg ttccaattct cttaaggtct tatccagata gtctgtcaaa
     9481 gaacgaatat ctgtaacacg atttcctcca atttcacttt cttcaatcat ctgcacataa
     9541 aatgctgtct tctgctcaag tgcaaataaa atcatactcc attcgctaaa attctgattt
     9601 tttcctacaa aagattctat tctatcagac tgcatcaatt tatttataag gtattcatag
     9661 atttcctttg cctctttata aaattctttc tttatattca tgtcatccat agaaagtact
     9721 gctttatttt tcttctcaaa actatagtac ttctgaagta tttctctatt taattgcatc
     9781 tctattttct caactgcata aaaaatttca taaatatgtg aaaattctaa aatatcagct
     9841 tctaaaattc ctactgcaca caacagtcta agatttctat aacctttaat tccagaaaaa
     9901 ccaaatctct cgaaaaattc atagaagaat gtatctattg acaaagaata ctcctgcttt
     9961 aacaattcct tttcttttag tgcatctcca gagtgaccgg cttttccctc tgctccggct
    10021 gaacctgaat cttcacgttt gctaattgtc cagtccttat gttcttcata aagagtattt
    10081 aatttctctc catcatctgc tgcttgcttc tgatttaaag catgattaaa cacatatcta
    10141 tcaaaataat agtagtttct atcactatta tccattggag gagaggtacc tatatatctt
    10201 attgtttctt catatatacc tctaattcca ccaagacctc ctgcaccacc agctcctcca
    10261 gagcctcctt ttcctgctaa accatgattt ccatcttttt tacttatagc actttttaat
    10321 aaaggagctt ttccaaggag ataaacctgt ccagacttgc ttccaagacc tgcatttcca
    10381 ccattacctc ctgtgcctcc agttccacct tgttttcctt tttctgcttt tacaacatag
    10441 tattttgtac ctttaacatt agcaaattct tttgactctt ttttagaacc tattttagca
    10501 tttgccccag cttctccagt tatccctgta tctccttttt ttccatcttg tccatttcca
    10561 ccacgtccac catctacgta aagtttaagt tctcctcttt tttctatttc tttaaaagat
    10621 aatatgatat cacctgaagt acctgcatta ttgccatttc ttccattcat tccgtcgtaa
    10681 atagaacttt ctccaaccaa tttttctgca tccttaccag atacgttgat tgaataagtc
    10741 ccttctggta tatgtaattt ttctgtaagt agtacaatat tcttacctga gaagacttct
    10801 ttctccatat tacagtcaat tttcaactca caatctccat acatatatat agatgacaaa
    10861 atattaagtt gataaccaga tattatttta gtcaaatccg ataaattcac tttaggtata
    10921 ggaatatata tagaaagtac attttctttc tcatatgatt ttatatatgt acaactttga
    10981 atcttctcta tcctatactc tgcatcttca agctttttgg cttcttcttc cctatattct
    11041 tcttctgcta tccaatattt gatatctttt aatgtatctt ctaaagtatt ttttagcaat
    11101 gattttaaca actcacttag accaacaata tgagtctcac tacagtaact atttaactct
    11161 aaaagaagac gtgtttcttc taatatattc actttctgta cgccatcacc atatgaaagt
    11221 tcttgtacaa actccataag ctctttcata ttattagtcc tactacataa atctgtatac
    11281 atcttatcta attgaatctt tttatctttt agactattta tagaagatag aatggaaata
    11341 gaatcttcca tacctttttt aacactttct atataaggaa ctaaaagttt ttctaggtct
    11401 ttataggtta catcaacagc tttttgtatt tctattttac aagaagctgt aagcatcaat
    11461 cccaaatctg ttttttcaaa tgtttcaggt agatttttca tttcatccaa caacttttct
    11521 ggctgtttat ctagtggatt acataattta atagatacac catctgataa tgctacattt
    11581 aataagtaca ctaattcctt acaaccgtcg taatctttct cttgactatc taagtcttct
    11641 tttgcttttt ccaatatatt tcttattttt tcttctgtta cattaccgat tagtgtttta
    11701 ggtattttat taataagaaa gaagaaattt tctttatact ttgcataatc accaagcata
    11761 tctttcaaat ttttgcctgt ttctatcaat aatgtatgtc tagagtccag tagggattga
    11821 tagtctaaga taactaccat ccctttcacc tttgaagatg tggaaatagt ttcccttaca
    11881 agcatagcac cagcaatcat atactctgct ccacggttgt caaaaaaacc tgggcaatca
    11941 cataatgaaa aatctctatt tgcaatatct acaacttctg gacataaagt cttagaatat
    12001 acaccatttc caatttcgac agctgaatct tttgcagtta tatatatttg tccagtctct
    12061 tcatccgttt tttcttccat cacacagcct tttaaataat ttatacaagt actctttcca
    12121 acgcctgaag cacctaggaa taacaaaatt gacttttctt ttaagtcata ttttctaagc
    12181 tcgtctcttc cttcttccaa aaggtttacg atttcactat aaagtttatt tgccatacat
    12241 ccatcccttt ctatatttcg gaacaatagc cttgatattt ttctttaata agattctctt
    12301 ctacctttga aacagctgtt gataacttag tatttggagc attaaatcct ccattgtctc
    12361 ctctatctcc atattcagcg tattctcttg ctggtcctgt tttaccttca ttccagcttc
    12421 caccactaaa ttctatatga tatttaatta tactatctaa cttataatcc caacttgtaa
    12481 aatatcctgt atagtataca gtcttatcta taccacttgg agaaaatgaa agtgtattat
    12541 atccgtgata gccaccactt cctccatatc cacctaaacc tggtctacca tttggacctg
    12601 cttctccatc tttcactgtt atatcacaat gattaattat tctatttttt tgttcctctg
    12661 aatctgaaat gaagtaaata ttacctttct taccagcatt tccacctctt ccagcatttc
    12721 caccagctcc acctttttca ccttttttgc cacttgtacc tttttcaaaa taccctttaa
    12781 acatctgtcc gtggaaataa cttgtatatc ctgcatcagc tccattactt ccagaagctc
    12841 ctgtttcacc attttctcca tctattcctt tagaaccatt tcctccattt gcaatcagtg
    12901 ttagacgata atttctaatt tcttctttaa tatataagta aatattacct gcatctctac
    12961 catcttcacc atttttccca tacatagaat tatctacaag ttctccgctc tttccactta
    13021 cattaatttt tacattacta cctacatcaa gattgttaga agcaagaata atatttttct
    13081 ctgatgcaaa gttcattcca aggttatcat ccatataaat agtatcttta gcaaagaaca
    13141 taaaagtaga aacattgtca tctacttttc tatttttgaa atattccaat acactgctaa
    13201 ttactaagtt tggaattaat gctcttattt caataccttt cttatcatta atattaattg
    13261 tttttactat aaatggattt aaaatgtatt taccaataaa ttctctaatt atctcattac
    13321 cttcttctcc atatgcactt aatcttgtta tactgtcttc taactcttca ttttgtgtaa
    13381 ctgtaacatt tgtcaaaatt gaattaaaat cacttacact atatacataa tcctttttat
    13441 ttgcaacttc cattaatttt tctctatttg gtaatgtaaa tacctgtttt tgcacttcat
    13501 aagaaactaa aactttttct aaattctttc ttattataat gaagttttgt tccattatct
    13561 tcttacttgc cttttctata ataaattcca tatgtacaga aggtaataaa atttccccag
    13621 ttccatgttc tgtatattta cttaaagcat caattatatc taagtttcct tttatcttat
    13681 tttgtaattt catgcaacta tcttgtaaga ataatccact ttgtttggct aatacgttga
    13741 taaatctctc tatagtaaga cttgttgtac tttccaacca gtttttaaat acttttacaa
    13801 cctcatcata ttttgctatt tcttgaatca ttgttatact tccaataata ttgataaaat
    13861 cttctgtata cgccttaaca taatcttcta ggctagatac gatattgttt tgaatttctt
    13921 ttatcaacgt atctaaaact aatcttgcat ctgcactaat tggatattgg aatgctgatg
    13981 tcttatcttt aacttcatct aattctagta cttgctttaa taagccttcc ctctcatctg
    14041 atgcaagtgg cttacaaaga tgaatatttt ttccattatc ttttagcaat tcttcaaata
    14101 accaagcttt ttcctctata ttactgtcta aattttcgca taattgttca tattttcttt
    14161 ttataacatt atatatttgt ttttctgttc ttcctaagaa actttttgaa atgaaaaaag
    14221 ttatactacc cttaaaattt tctttatgaa gacacatatt taactgttgg aataggtcta
    14281 gtacttttgt aagtctagaa tctaatatat ctgcttctgg tatcaataca attactgcac
    14341 atatagatgc agaattttta aatacttcac ctaatgaaac tgctgcacta atatcatggg
    14401 ctactcccct attgtcgcta aaacctgcag tatcacataa ttttgcatcc attccttcta
    14461 ttggttttct catatttaca atgttcgggt ataaagtttg agaaacagct cctgaaccaa
    14521 tctctgccac agggtctaag caatcaatat atgatatacc agtatcttca tcttctactt
    14581 ccatcattac acatcctgca agatagttaa ttgcagtact tttgccacat cctgtcccac
    14641 caagaattag aattaaattt tgattttcag ccttatttat tgctactttt ccttcattta
    14701 ataagagtgt tatttcttct aaaagttgat tgttattttc tggcatttat tttccccctt
    14761 ggttatatat tttatttaaa taaagtttaa tcccttaagt agattgcaaa gtttttagac
    14821 tatatttaaa tttgtttttt attttttatt tttaataaat ttgctttttc actaaatttg
    14881 ctttttcact caataattat tcttctttgt ttattaaaat aactgtgttg tataatataa
    14941 tctttaccaa cacagttatt ttatttaatg cgcttatttt attgtatttt atgtaataca
    15001 gttgtttttt atattatatg tccattattt tcctgtggtt attacatata gatttttaat
    15061 tatatttgta ccattaattt aaacattcct atactactgc ttaagtttta cacctatacc
    15121 agcttgaata ttttattttt atttgcttaa atagttcaac tatactggtt taaataggtt
    15181 atctttattt gcttaaatag ttcaactata ctggtttaaa taggttatct ttatttgctt
    15241 aaataagttc aactatactg gtttaaatag gttatcttta tttgcttaaa taagttcaac
    15301 tatactggtt taaataggtt tcctttagtt taaataattc acctatacct acttgaatag
    15361 ttcactttta tttgcttaaa ttctatacca ataccggctt aaacattata ctaccatgat
    15421 tgtattgtct atcttcaact acaataaaaa caggttctaa atctttagta gatgttattt
    15481 ttaattctaa aaaaatatta tcagttggta atatttttac atcataatta tttaaattat
    15541 tattcgtgat actattaaat acatcacctt gacgttcaaa aaaaacatta tcaactatta
    15601 caaaataaac atcttctttt ctactatttt caacctctat agtattttgt ttagttacat
    15661 ttagtctcat cttccacctc ctattaatat tacttaatta tattaatact tgtaattgtt
    15721 gcattatgtt catcacaagg aagaggttta aaaacaacta tctctgctaa ataattctgt
    15781 ggaggatttg taactgaagt agcattgcaa ataaatttaa aatttgttcc tgctacaact
    15841 tgagtagaaa caataagtgg ttcataacca accccttcta acattcctac agcttcatta
    15901 aatactttta aatcttcttc ttcgacagct ctgaattttg accatccacc aacaaaaaga
    15961 ctcatataaa aacctccttt atatatatat ttatttatat atagtagtac caattttctc
    16021 aatattcaat attttatgtg taactggtca atgaaaatgc ataactggat aaaaatactt
    16081 ataaaataac ttaactttac atataaatag cttactttac atgtaataag attattctac
    16141 aatatgaaat gcatcttgtt ttgaatatat tctaaataca ataatggata ttttcaatta
    16201 ttataattag aaaatatcca ttacttaact tgttttattt aaaatcacat agagacttta
    16261 ttattttttt tattgcatga atagctattt caggtatcat aaggtgatat accccctcct
    16321 tgaaaccaca aaaaacatga caaactttac gaatcttggc ttaaaagacc acaggtacat
    16381 tccttatttg tggattggag tagccataac aagcaaattt tacaattttt atgatgacaa
    16441 aaatcaataa aatcgcttag tatataacac gaaacactac ttgttactat taagaatagt
    16501 tagataggaa gtatgcatta taccaataaa ggaatcaata aagaaagtta aaacatccct
    16561 acaaattaaa aatctgttac atttttctat atttaaacgt tatttactta caattggaga
    16621 aataacaggt tttccttctc ggtcaagtaa aggcgtaaat ccggctgcat tgccataata
    16681 gtggaataca tagttcacac ctgtttcttt atccacccaa atctccgttg catttatttt
    16741 tccttgtgaa tatacttttt caaatctatc ttttcccatt ttataatccc ccatattttt
    16801 gattggtcta taccttcttc acttaagtcg aaaaaataca taacttattt ttttaattct
    16861 aatatgagca cgtaaaaatg tcaaacagct tatttaattt cataacttca tgttactgtt
    16921 cgtaaccttg tctgtttact tacactgcat tacagcacca ttttatcctt tacattattg
    16981 tattgcacac agtaaaaatg cttactttat gggatttttc aactaaaaaa aggattttat
    17041 ctccacaaaa catttactat ttgttacaat atacctaatt ttaatctcat atcaacattt
    17101 aatgaagata taaccttttt caaatattgt aaagactcct taaaaatatt agaaaaagat
    17161 tctgcaaatc tctcaacatc tccttttttt aaatacctaa gtatctcaaa acatacatca
    17221 aaacgtgttt tcaaaatttc attatattct tcatgaaaaa atctctctaa atagattcca
    17281 aatatcatat attgttttaa atgcaaaaat gtttcctgta atacataaat ttgtgtataa
    17341 tctactatct tacaaagtaa aacaaaatct ggataaacct tacactcttc acaattattt
    17401 tgtaagtacc ttaaatgagt ttctatttta tctacagcta tcaaatccat tttatttcct
    17461 agtaaaagta ctacatcctc aagagtaatc gtaacaaact ccatagcatc caaaagcaaa
    17521 ctcaaccttt tagctccttc ttctgtattt atataatcat catcatattc agaaaaaagt
    17581 gttatatatg ttcccttgcc attaattgtt ttagcaattc ccaattcatt tagctcatta
    17641 taggcacttc taactgttgg aagagataca ttatatctat taattatcga agtaattgat
    17701 ggtagacaat ctcctatttt aatattgcct tcaaaaattt gacaaattac atctgaaaca
    17761 attaagtcat ataaataaca tttttccaac gttacagaat taaaactact tgtatctata
    17821 ctttgtggag cacgattttc taccatttta agtatggatt ctctacaagc tttatacata
    17881 tatgttaata gttttttaaa ttcaattaga ttcccgtcca atccatataa gtaagttttt
    17941 cttacctgct ctatgtattt attacgaaaa aagtctatac aacaattatc caagttatcg
    18001 aaattatagg ccaattgtga aataatgaca tcatgctgaa taatctcaca aaaatggtga
    18061 atcaggaaat tatttgtttt acttattagt ttatttatat attttagcct ttgttcttta
    18121 aagactaaat aattagaact atttttttcc atacaatcga taatctcgtt taattcttca
    18181 aagtctttag atgtaaatct tttaatattg tacatagcaa tagaaggaaa tattaaacta
    18241 aaaatcttat atatttctga tatttgtaaa atcttctcat ataataagtc agattctatt
    18301 gattgtttac tcgtactctg gattaaaact ggtctagaac cagcttttgt ctctataaag
    18361 cccttttctg agagcatttt tattacagtt tttatagtat tttttgatac catatattct
    18421 tctgacatgc tttttataga atcaagccta tctccaatag taattttacc agtataaatt
    18481 tgttgaatta tatgctcaaa aataactaaa tattgtggtt ttctcttcat atttttcctc
    18541 ttcgataaat tagatttatt gttttttgta aggtatattt acttttattt aatagggcaa
    18601 aatagttgct tattttttcc tcataatcta taattaatat aaaccattta tatatatggt
    18661 ctaagtttta taatactgta atgcagtaaa atagcatatt gtagctatac tacagcaaat
    18721 taacatacca ttgtagtata ttaattggaa taattttaag tacttcaaaa ttccatcaaa
    18781 acatatatgt gtttttgata ttatgtatat tcgttaatag aatagctata cttgttttac
    18841 ataataaatt atatgatact tgctatatgt aattactata tttaatacac ataactatta
    18901 tacttactat atgtaattac tatatttgat acacataatt atgatactta ctatatataa
    18961 ttactatact tggacacata actattatac ttgctatatg taattactat actttataca
    19021 catagttgtt atacatgcta tatataatta ctacacttgg acacataact actatattta
    19081 ctatatgtaa ttatactata cttggacata tagttatcat acatactata tgtaattact
    19141 atacttggac acattattat atttgctata tgtaattata ctatacttgg acatatagtt
    19201 atcatacata ctatatataa ttactatact tggacacatt atcatacata ctatatgtaa
    19261 ttactatact tatacacata gttatcatat atactatata taattactat acttggacac
    19321 atagttatca tacatactgt atattaagat ataatacttt ttaaatttta tataacacag
    19381 acacatgcaa taaaaaagaa ggtatccata ataagctctt tgcatgtacc taaataaaaa
    19441 ctgtaaaata gaatatataa aatctaatct attttaagta aatacaattt cactttaaaa
    19501 gaggatgtac tatgaaatac catatatttt taatcataac tactatatta ataatcgaac
    19561 tatgtaacat ttacatatgc aaaatagcat cttttggaaa caaaaatata actatacata
    19621 agaattacaa atatattttt ttaataaaac cattaacctt cttgataata atattcacaa
    19681 aattaccatt accactatat tatatattgt tttacatagt aagtcataaa ttgctcttaa
    19741 gcagaaaaag ttctactaac aaccatttta ttcccaatat ggcagtaata agtttttgta
    19801 ttttacacct tattactgta agtacaatat cacttgttaa aaatacaagt ccagatataa
    19861 tatttgcaaa tatgacctat aagttaatta gtattttaat agcttctatt ttagatttta
    19921 tcttatggta ctctatgaat aaaataaaag agcatattta tataataact caaaatcaaa
    19981 tagagttcag tctattttta aaatttatct ggttcacttt aggatatata ttatttgata
    20041 gtatacaaat tacatttgct ccaccaagtt ctctaacttc ctactttata ttgggaagta
    20101 acatattatt aatcttacaa ctaattatat tcatcaatca tatatattcc attgaaaaaa
    20161 atatttattt agaaaaagaa cataatagac taaaaatgat aaaacaagaa caattactaa
    20221 aagtaaattt attaaagaaa aaagctaaca ttgatgaatt aacatcagca tataatcgaa
    20281 aatttgcttt tgaaaaaata cagcattaca ttgataacaa tttattattt tcattgatat
    20341 atatcgattt ggataaatta aagacgataa atgatacata tggacattta gctggggata
    20401 actatattat taacttctca agtatagtta ttgaaaactt aggaatagat gacatatttg
    20461 ctagaatagg aggagatgag tttatagtta ttatgcctaa atctaatgaa aacaccacaa
    20521 aagaatttat agagaaaata agtaaaatta tggataatac gatatctttt agctatggta
    20581 ttgtagaagt ttctagcgaa aacaaaatga ctgtagaaga aattattaat attgctgata
    20641 ttagaatgta cactaataaa caaaataatc ataaaaggaa caataactaa aatgaatatt
    20701 tacatgagct tacttgtagt ctcactatgt tttttatctt attatctcta tcattataat
    20761 ttttttaaat atctggacat aatgattaat aac
//
LOCUS       DAGSWI010000066        20204 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.066, whole genome shotgun sequence.
ACCESSION   DAGSWI010000066 DAGSWI010000000
VERSION     DAGSWI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.066"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(306..767)
                     /locus_tag="MJF80_002232"
     CDS             complement(306..767)
                     /locus_tag="MJF80_002232"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBZ0280890.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLD"
     gene            complement(872..2065)
                     /locus_tag="MJF80_002233"
     CDS             complement(872..2065)
                     /locus_tag="MJF80_002233"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBZ0280891.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(2088..2753)
                     /locus_tag="MJF80_002234"
     CDS             complement(2088..2753)
                     /locus_tag="MJF80_002234"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBZ0280892.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(2787..3899)
                     /gene="ribD"
                     /locus_tag="MJF80_002235"
     CDS             complement(2787..3899)
                     /gene="ribD"
                     /locus_tag="MJF80_002235"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBZ0280893.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(4031..4152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4341..6824
                     /gene="recQ"
                     /locus_tag="MJF80_002236"
     CDS             4341..6824
                     /gene="recQ"
                     /locus_tag="MJF80_002236"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBZ0280894.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFINTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      6913..7024
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7166..8467
                     /gene="thiC"
                     /locus_tag="MJF80_002237"
     CDS             7166..8467
                     /gene="thiC"
                     /locus_tag="MJF80_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBZ0280895.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            8521..8715
                     /gene="thiS"
                     /locus_tag="MJF80_002238"
     CDS             8521..8715
                     /gene="thiS"
                     /locus_tag="MJF80_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBZ0280896.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            8708..9511
                     /gene="thiF"
                     /locus_tag="MJF80_002239"
     CDS             8708..9511
                     /gene="thiF"
                     /locus_tag="MJF80_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBZ0280897.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLFENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGKGLMAPRVAICANHMANLATQILIDM"
     gene            9574..10344
                     /locus_tag="MJF80_002240"
     CDS             9574..10344
                     /locus_tag="MJF80_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBZ0280898.1"
                     /translation="MDKLILGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            10361..11464
                     /gene="thiH"
                     /locus_tag="MJF80_002241"
     CDS             10361..11464
                     /gene="thiH"
                     /locus_tag="MJF80_002241"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBZ0280899.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            11465..12154
                     /locus_tag="MJF80_002242"
     CDS             11465..12154
                     /locus_tag="MJF80_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBZ0280900.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            12342..13736
                     /gene="yfcC"
                     /locus_tag="MJF80_002243"
     CDS             12342..13736
                     /gene="yfcC"
                     /locus_tag="MJF80_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBZ0280901.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            14253..15620
                     /locus_tag="MJF80_002244"
     CDS             14253..15620
                     /locus_tag="MJF80_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBZ0280902.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            15645..16253
                     /locus_tag="MJF80_002245"
     CDS             15645..16253
                     /locus_tag="MJF80_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBZ0280903.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            16301..16672
                     /locus_tag="MJF80_002246"
     CDS             16301..16672
                     /locus_tag="MJF80_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBZ0280904.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            16851..17189
                     /locus_tag="MJF80_002247"
     CDS             16851..17189
                     /locus_tag="MJF80_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBZ0280905.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            17655..18158
                     /locus_tag="MJF80_002248"
     CDS             17655..18158
                     /locus_tag="MJF80_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBZ0280906.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            18205..19164
                     /gene="moaA"
                     /locus_tag="MJF80_002249"
     CDS             18205..19164
                     /gene="moaA"
                     /locus_tag="MJF80_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBZ0280907.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLLENKCSDTNRRMYEIGG"
     gene            19168..19638
                     /gene="moaC"
                     /locus_tag="MJF80_002250"
     CDS             19168..19638
                     /gene="moaC"
                     /locus_tag="MJF80_002250"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBZ0280908.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            19674..20105
                     /locus_tag="MJF80_002251"
     CDS             19674..20105
                     /locus_tag="MJF80_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBZ0280909.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         7867 a         2198 c         3446 g         6693 t
ORIGIN      
        1 ataaaataga taattaatat actagtaata tatgctataa ataaaaacaa tagctaaaat
       61 agtaatatat atgtttttat aatattttat gtttagggtt atatttgtaa ttattttaag
      121 cttctaaaag tagatttgat ggataacgta taattttaga aggatatgca atttgtttga
      181 taagaacgta catatgatta aattaaaaat attaagtacc aaaaagaaga aagatagttt
      241 ctccctttgg tacttaatat tttgtatcct ttacactaat ttttaatatt tattaatgat
      301 attatttaat ctagagactt aaataaattt gccatttcta gcgcattcat agcacaatca
      361 taacctttat ttccagcttt tgtacctgct ctttcaatag cttgttcaat attttctgta
      421 gttacgattc caaatataac aggtaattca ctgtctaaag atactttggc tatcccttta
      481 gagacttcac tacatacata atcataatga gaagtagccc ctcttataac acagcctaaa
      541 cagattatag catcatactt accactttta gccatttttt gagcaacaag aggaatctca
      601 aaagctccag gtacccaaac tatttctata ttttctggag aaacgtcatg tcttaaaaga
      661 cagtcttcag ctcctgataa aagttttgat gttataaatt cattaaaacg agaatttatt
      721 atacctattt ttaaatcttt tccaataagt tttccttcat atatcatttt tatttcctcc
      781 aattttattt atgttataga tttatattta taaatatctt taatttaatt ataaaaaact
      841 acttagtaga aaaatatcat catataatca tttatataat atctaaaaga tgtcccattt
      901 tatctttttt agtttttaaa tatagcttat taaagtcatt aatttcttca ttaataggga
      961 ttctattttc gatttttatt ccatatttct ctaattgatt tattttatct gggttattac
     1021 ttaaaagatt tatacttttt atgtttaaat tttttaatat ttgagcagcc atatagaaat
     1081 ctctcatatc ttcttcaaat ccaagcatta aatttgcttc aacagtatcg taaccttctt
     1141 cttgaagttt ataagcctta attttattta aaagaccaat acctctacct tcttgcttca
     1201 tataaattaa aactccactt ccattttcca caattttctt catagaagat tctagctgta
     1261 atccacaatc acattttaga gagtggaaca catctcctgt taaacattca gaatgaagtc
     1321 taactagagt attttctata tttatatttc cataagtaag ggcaatgtgt tcttcatttg
     1381 aataagtatc tctatatcct atgatttcaa aatttccata ttttgtcgga aggaaagcac
     1441 tagatacttt ttctatagta gtttcattta ttttgcggta ttctataagg tcttttatag
     1501 tgatgatttt taaattatgc ttttctttga aaatcattaa atcatcaact cttgccattg
     1561 taccatcatc tttcattatt tcacagataa gaccaatctc tttaaaacct gcaagtctag
     1621 caaggtctac tgaggcttct gtatgtccat ccctgacaag aaccccacca gtttttgcta
     1681 ttaaaggaaa gatgtgacca ggcgttttga aatctttact tgtagaattt tcatcacaga
     1741 acaattttac agtttcagct ctttcatatg cagatatacc agttagagta tctgctccat
     1801 ctatagtaac agtaaaggct gtttgaaatg tatctgtatt ttctctaacc ataagaggta
     1861 tattaagact tttaaatttt ttttcagtgg caggtagaca aattagacct ctaccatatg
     1921 tagccataaa atttattgat tcaggagtag ccatttcagc tgccatcaat aaatctcctt
     1981 cattctctct actttcatca tcaactacga ttaccatttt cccatttttt atatcttcaa
     2041 tagcttcttc aatagtatta aacatgtaat tcactccttt attattttta aaaaccattt
     2101 ttaaataaaa aatctttatc tatatttgat ttatggttac tactattaga atttaattcc
     2161 ttgtagttat tagccataaa attattaaca tactttccaa ttacatcgct ttctaaatta
     2221 acgaaatctc caacattctt tgtcaagaga atagtgttaa ttttagtatg aggtataata
     2281 gaaaccttaa atatttcatc atctaaatat gatatagtta aacttacacc atcaatagca
     2341 acagaacctt tgagtatcat tgaggataaa agttttttat caggtcgtat acttactaaa
     2401 actgcatttt catctttttc aaatttgcag atagttcctt taccatcaac atgacctgtt
     2461 actatatgac caccaaatct tgaagaaaga ctcatagctc tttcaagatt taccttgtca
     2521 tttgcattta ataaacctaa atttgtggaa cgaattgtct ccatcataac atcggctgta
     2581 aattcatttt ttccgaggtt tgaaacagtt agacaaactc catttacggc tatactatct
     2641 ccaagatttg tgccatctaa tattttattt gatagtatgg ttacttttcc actcttacca
     2701 ttagcagtaa tttttctaag tattccaact tcttctacta tgccagtaaa catcctaacc
     2761 tcctatatga atgtattaac aaaaatttaa tttaaaacat ctgattctat aagaatatcg
     2821 ccatctatca aagttacatt attgattgtt aactgtgttg aatctgacaa atgatttatt
     2881 ccttctccag aaacgaatgt aggtgcatct ttaccaccta taatcttggg cgatagaaat
     2941 aatttgactt tatcaactaa cttatttcta aagagactgt cattgagagt agcaccacct
     3001 tcaactagaa cagaatcaat gtttaactct cctaatttat acattacaaa ctccaaatct
     3061 actaagttgt tttttgaagg agaaattatt gtttcaacat tattttcttt taacatagat
     3121 aattttatac tttctttgca acatgtgaaa actatagttt tactggtttt atctttaacc
     3181 aaattactat taagtggcaa ctttaggtgt gtatctatta ctattcttgt aggatgagac
     3241 actttttctt gattgagcct acaagttagt aaaggattat cgttcaaaac tgtatttatg
     3301 ccaaccatta tactttggta tttatttctg tatttatgag ttagatacct tgatgattca
     3361 ttagatatcc attttgattc tagtgatttt gtggcaattt taccatctag acttactgct
     3421 gattttacaa tacaaagagg tcttttgttt tttatatgat aaaaaaatgc ctcatttaaa
     3481 tctcttgctt catcttctaa tattcccaca gtaacatcaa tattgttatc ttttaatttt
     3541 tttactccat taccacaaac aagtggattt gggtctaatg tagatattac aactctctta
     3601 atcttatttt gtataatttt atctacacaa ggaggcgttt tcccataatg agaacatggt
     3661 tctaagttta cataaagagt agagtctttt agactttgtt tagcactgtt tatagcattt
     3721 acttcagcat gattagagcc aaatttctca tgatatcctt tgcctattat attactatct
     3781 ttaacaatta cacatccaac taatggattt ggatttacaa agccttctcc atttttagcc
     3841 aattcaatag cttttttcat ataataaata tctttttctt tttgattcac aaaattcaca
     3901 atatgtacac ctcctgttta attggataat ttttaaacaa aaaaccctga gaattaactc
     3961 agggtttaga ataatctaca ataaatgttt aataaatgta gaacaaaatt gctctacaaa
     4021 aacactcatt aaatatcttc ttccatccag actttactgt cggctttgga atttaaccaa
     4081 atcaaccaag ttttacattg gctcgcgggc tataccgccg attgggaatt tcaccctacc
     4141 ccgaagatta agtattaaat ttatatttag aatgtatcat aaattatttt actttgcaaa
     4201 ctattttttt aaaaaatata tgttaatata taatatagaa tgtctataaa ctttgaaata
     4261 ccacgattgt gaatataaaa ataaaaattt gaacataatc aggaaagaag tctaattaat
     4321 ttaaattagg agagtaatat atgaataaaa aacttttaga aatactatca aaatactatg
     4381 gatatacaag ttttagaaaa ggtcaggaat ctataataaa ttctatctta agcgaaaaag
     4441 atgtgctagc tattatgcca acaggagggg gaaaatcaat atgttatcaa attccagctt
     4501 tgattttaga tggaatgaca atagtaattt ctccacttat atcacttatg aaagaccaag
     4561 tagatgcctt aaagactatg ggaattagtg ctgcttttat aaatagttca ttaagcagca
     4621 aagaatttaa tgaaatatta aataatatta gaaaaaataa ttataaaata ctctatattg
     4681 ctccagagag attagatgca caagagttta ttgagctaat aaataataac aatgtaagtc
     4741 aagttgcgat agatgaagca cattgtgtat ctcaatgggg gcatgatttt aggttgagtt
     4801 atagaaggat ttctgatttt ataaaaaatt tgcctaaaag acctatagtt acagcattta
     4861 ctgcaacagc ttcagaagag gtaagaactg atataataaa tttactgtgt ttagaaaatc
     4921 cagattatta tataactgga tttgatagag agaatttatc aataaatata gtaaaatcat
     4981 ctagtaaaaa taagtatatt ttagattata ttcaaaatca taaaaatgag tctggaatta
     5041 tatatgttgc aactagaaag gaagttgaaa atatatataa tggtttatct aagagaaata
     5101 tatctgtgtc aaaatatcat gcaggtttat ctaaaaatga aagaagtaaa aatcaagaag
     5161 attttataaa tgataatgtg gatataatgg tagcaacaaa tgcatttgga atgggtattg
     5221 ataaacctaa tataagatgg gttatacatt acaatatgcc tcaaagtata gaaaactact
     5281 atcaagaaat aggtagagct ggtagggatg gagaaaaaag tgagtgtgta cttttatttt
     5341 ctccaggaga tgtacatata caaaaatatt taattgatat tggagttgaa aatcctgaaa
     5401 gaaaattatt tcagcataag aagcttcaat acatgattga cttagtttac agtaattcat
     5461 gctatagaaa gacaatattg agttattttg gagaaaatta tatagacaat tgcaataatt
     5521 gtagcaattg tttagtagag ggtgaaatag tagataaaac tatagatgca caaaaagtca
     5581 tttcatgtgt agctagaatg aaaagaagtt atggtgttac tactattgta gatgtgctta
     5641 gaggttctaa gaataaaaag attttacagc ttggattaaa tactttgtct acatataaca
     5701 ttatgagaga ttattctagt gaagacttaa agaattttat aaatacattg gtttctcatg
     5761 gttttttaga tttagttgag actctaggca atggaaatag aggtagtttt cctactataa
     5821 gattaaatga gatgtctatg aaagttttaa aaggtgaagt gaaagtagag tttaaagaaa
     5881 ttgttatgac aaaatcattg gaagtagagg atgagttata taatgcttta agagaactta
     5941 gacattcaat tgcaagtgaa gaaaggatag ctccatatat ggtatttgga gatggtacac
     6001 ttagagccat gtctagtagt tatccaatta ataaagaaga aatgctagat atctctggag
     6061 taggagaaat taaataccaa aaatatggta gagattttga agcagtaatt aaagaatatg
     6121 tcgaaaaaaa taatataaat aaaaaaatag atgaaaaaaa tgtcgattca aagaacgtta
     6181 atagtgattt cttagaggtt aatacagata aagttttata tgaaaaactg ttagtcatta
     6241 gagaagaata tgcaacaaag gaaaaattac ctgcatatat gattttagcc aataaggctt
     6301 tgaaagaaat aagtggaaga tatccacttg atgaagaaca attaaaagat atttctggca
     6361 taggtggagt aaaatttgaa aaatatggag ctagtatttt ggcagaggtt aataaatatg
     6421 taaaagaaaa taatattaat gttatttggg agaaaaaggg caaaaggaag ttaatattag
     6481 atggagaaag tagaaaaaat aatgaaatag cattagattt gctaaatcaa ggaaaagata
     6541 tacaaagtat cagtcaagaa atagaagttt cactgtcaac cattattgga tatgtgcatg
     6601 actatgtaaa ggatggacat agtatatcat ttgaattagg actagaaaat ttttataatg
     6661 aagataaaaa gaagatgatt ttagaagttt gtgaaaaagt tggctatgat aatttaaata
     6721 acataaaaag aaaacttcca gattcaataa agtatgaaaa tataagagct gtgatacttg
     6781 actcttatct tgaaaactta aaaaataata ttatagcaga gtaattttta acttaataaa
     6841 aaataaaatt tgtattattg tgttgaacta ataattttgc tgtgttatac tgaatttgta
     6901 aaaataatga aaaattagag ctaggggcgc tagaattggc tagctgagag ataaagccgt
     6961 aacttacaaa ccctaataac ctgatcaaga taatgcttgc gaagggaagc tttttaatat
     7021 aattttatta tataaatagg caaattattc aatgttagga gaaatttgtc taagtgtata
     7081 tacgattata agagtgcttt ccaaacgagg aaaagcactt tttttatttt caaaaactat
     7141 aactttaatc ttggaggatt gtaatatgaa ctatacaaca caaatggatg ctgctagaaa
     7201 aggcataata actaaagaaa tggaaatcgt ttctcaaaaa gaacaggtag atgtaaatga
     7261 attaagagaa ttaatagcaa atggacaagt tgttatacca gctaataaaa atcataaatc
     7321 tttaagtgca gaaggtgtag gtaaaaattt aagaacaaaa ataaatgtaa atctaggaat
     7381 ttcaagagat tgtaaggata tagaaaaaga acttgaaaaa gttagagtag caatagacat
     7441 gaaagctgaa gctataatgg atttaagtaa ttatggcaaa actagagaat ttagagaaaa
     7501 ggttgttgaa atgtcacctg caatgatagg ttcagtacct atgtatgatg cagtaggata
     7561 tttggaaaaa gaattaaaag atataacaga agaagagttt ttaaatgtaa taagacaaca
     7621 tgctattgat ggagtagact ttattactat acatgcaggt cttacaagaa gtgtttgcca
     7681 aaaaataaaa aatcatgaga gattgactca tatagtatct cgtggaggtt ctttattgtt
     7741 tgcatggatg gaattaaata ataaggaaaa tcctatttat actaattttg ataaaatatt
     7801 ggatatatgt gaagaatatg atgttacatt gagtttagga gatgcttgta gaccaggttg
     7861 tataaaggat tctactgatg gtgttcaaat tcaagaactt gtagtattag gtgaacttac
     7921 taaaagagca tgggaaagaa atgtacaagt aatgatagaa ggtcctggac acatggctat
     7981 tgatgaaata gaagcaaatg tagtattaga aaagagatta tgccatggag caccttttta
     8041 tgttcttgga ccattggtta cagatatagc accaggttat gaccatataa caagtgcaat
     8101 aggaggagct ttagcatgtg ccaaaggagt agatttttta tgttatgtaa ctccagcaga
     8161 acatttaaga ttgcctaatt tagatgatat gaaagagggt ataatagctg caaagatagc
     8221 tgctcatgca ggagatatag ctaaaaatgt taaaggtgct cgtgaatggg ataataagat
     8281 gagtaaagct agagctgatt tagactggtg tgagatgttt agacttgcaa tagaccctga
     8341 aaaagctaag agatacagag atgaatcaac tcctactcat gaggatagtt gtactatgtg
     8401 tggaaaaatg tgctcaatga gaacagttaa gaaaatatta aacaatgagg aacttaatct
     8461 aatataattt ttggctagta tgactggaat aaaaagaagc tgaaaagagg aggtgattta
     8521 gtgaaggtta atgggaagga aattgaattt gaaaaagatt tgactgttat agatttatta
     8581 aataaatata atttaaaaag tgatagagtt gtagttgaag tgaatttaga gataattgaa
     8641 gaatctaact acaatacata tgtattaaaa gacgaggaca ttgtagagct tattagcttt
     8701 attggaggtg gttaaaataa aaatatttgt aaatgaatta ttaacagatg ttgaggaaaa
     8761 tacaactgct tataaagtta gagatagcta taacaataaa tgtgatgtat tggtgttaaa
     8821 tggatatcca attaaaacag atatgcccct ttttgaaaat gataaattaa cacttataca
     8881 aaaaggagtt aagccatcac ttgatgaatt agaaagcttg ttgattgcta gacatactcc
     8941 aaatatacat aataaattaa aaaaagggaa agttgctata ttgggattgg ggggacttgg
     9001 ttccaatata gctatctctt tagctagaat aggagtggga gaacttttac ttatagatta
     9061 tgatgttgta gaaccatcta atttaaatag acagcagtat tttatagatg atataggaaa
     9121 actaaaaact gatgccatga tagaaaatat aaaaaaaata aatccattta taaaattaaa
     9181 taaacgagat atttttttaa acaagaacaa tatggatact attaaggatt cagatttaat
     9241 aattgaggcc tttgatgatg cttcatgtaa agcacaggta tgtaattatg tattaataaa
     9301 tttaaaagat aaatatttaa tagcatcatc tggtatggca ggttactatg attcaaatat
     9361 aataactact aagaagataa aagacaggtt ttatatatgt ggggattttg tgaatgaagc
     9421 taaatttggt aaagggctta tggctccaag ggttgccatc tgcgcaaatc atatggcaaa
     9481 tttggcaacg caaatactta tagatatgta aatattaaaa ttagatataa aaaagattga
     9541 atattaaaaa aatttaacgt ggagggaaat aatatggata aattgatact tggtggacat
     9601 gaatttaata gcagattatt ggttggaaca ggtaaatatg gttcaaataa tatattgcct
     9661 gaggtaataa aagaaagtgg aagtgaaata ataacaatgg cacttagaag agtggattta
     9721 gacaataaac aagaaaacat attaacgtat attccaaaag agatgacaat acttccaaat
     9781 acatcaggag ctactaatgc agaagaagct gtaagaatag ctagaatatc tagaaaaatg
     9841 ggatgtggag attttataaa aatagaagta atatcagata ctagatattt acttcctgat
     9901 aatgaagaaa ctataaaagc tacaaagata cttgctgatg aggggtttat agttcttcca
     9961 tatatgacac cagaccttta tgctggaaga agacttatag aagctaatgc agcggcagtt
    10021 atgccacttg gagctccaat tggttcaaat agaggtcttc aaatgaaaga aatgataaga
    10081 ataatgatag atgagttgga tataccaata atagtagatg caggtattgg aaaaccatct
    10141 caagctatgg aggctatgga aatgggagct gatgcagtac ttgtcaacac tgctatagct
    10201 tcagcaggag accctgtaca aatggctaga gcatttaaat tagctgtaga aggtggaaga
    10261 gaggcatata ttgcaaaaac aggaaatgtg tcagaatttg caaatgcatc ttctccttta
    10321 acaggttttt taggcaattt atagaaagtg gtgattggtg atgagttttt atgatgtaat
    10381 agaaaaatat agggattttg attttgatgg atatttaaat aatgttactg ataatgatgt
    10441 tttaagaagt ttatcaaaag acaagcttga agattttgat atattaaatt tgctttctaa
    10501 aactgcagta aaacatttag aagatatggc acaaaaagca cataaattaa gtgttcaata
    10561 ctttggtaaa acagtatgtt tatatacacc aatgtatata gcaaattatt gtgttaatca
    10621 atgtgtttat tgtagctata atataaagag tggaataaaa agaaaaaaat taactatgga
    10681 tgaaattaga gaagagggag aagcaatttc aaaagaaggt ttcaaacatc tacttgtttt
    10741 gactggagaa agtagttttc actcaagtgt agaatatata ggtgaggcta tagaaatatt
    10801 aagagagaaa tttccctcta taggtataga agtttatcca atggaagtag aagaatataa
    10861 gtacattgta gataaaggtg ttgaaggatt aacagtatat caagaaactt atgacgagga
    10921 aatatataag agagtacata taaaaggccc aaaatcaaat tataaattta gactagatgc
    10981 tcctgaaaga ggggcaaaag caggtatgag gactttatca ataggtgcac ttttagggct
    11041 aaatgatttt agaaaagaaa ctttttttac aatattgcat gggaaatact taaaaacgaa
    11101 atatcctcat attgagcttt catattcaac accaagaatg agacctttta aagggtgttt
    11161 tgaggaattg gtagatataa gtgatactga tttagttcaa gctatggtat gtatgagatt
    11221 atttgaccct catgcagcta taaacatatc cactagagaa aatttagaaa tgagaagtca
    11281 tataatacct ttaggagtaa ctaaattgag tgcaggtgta tctactgatg taggaggtca
    11341 ttctcaagat gaacatgata ctgctcaatt taagataaat gatgaaagta ctgttaaaga
    11401 tgtggagaaa atgctaaact caattggtta tcagcatgtg tttaaagatt gggaaagatt
    11461 ttaaatgtat ctgataacaa acagaaaatt atgtagtgag gaacgatatt tagaagttat
    11521 taaagagtct atattgagtg gtgtagaaaa tataataata agagaaaagg atttggaata
    11581 tcaagagcta agaaaattat atatgaaaat aaaaactaaa atcaattgta tagattttca
    11641 agagcaaata tcagatgaaa gtttgaaaac taatataaat caaaaagagt gtagaaataa
    11701 attcaaggtt aattttataa taaatagtaa cattgaattt tttgaaaaag tggattgtca
    11761 aggtatccat cttcctttta aattgttctt gaatttaatt gaaaataagt ataattttaa
    11821 tgaaaataaa atactaggat tatctttaca taaagtagag gaagtagatt atttagaaaa
    11881 attaataaga aatcaaaata taaaaataga ttacataaca ttatcacata tatatgaaac
    11941 taaatgtaaa gaaggattaa atccaaaagg aattgagctt ttaaaagaag ctaaaaagat
    12001 tactgatatt aaaataattg cacttggggg aattttacca tctaatgtca aggaaacatt
    12061 aaaatactgt gatgattttg ctataatgtc aactataatg aggtctaaag atattaaaaa
    12121 aaccatatca aattataatg aaaagttgaa ttagtaaata tagtatattg aatttttaac
    12181 ttaaataaaa ataaaaaagt tatcttttaa attttaaaag gtagcttttt tattttatat
    12241 ctttgctaat aaaaaattgt aaaaagggaa aactttagtc agagagatta aacaatatcc
    12301 tagaaagatt taaaatttag taaagtatgg aggacattat aatgactgac agtaaaaaga
    12361 aacgatttat tataccacac acttttacaa taatattttt actaatagtc ttaatggcaa
    12421 tattgacttg gatagtacca agtggtgaat ttagtagaga agatgtagaa ggtagaatgg
    12481 tagttgtacc agggacttat caaagtgtag aaagtaatcc tcaaggattt gcagatgttt
    12541 ttaaagcacc aatagaaggc tttatcgatt cagctgaagt tgtaggtttt gttttaatag
    12601 ttggaggtgc ttttggtatt gtaaatagga ctggagctat agaagcagga atagcttcgg
    12661 ttatctctaa gtttaaggga aaagaacttc ttataattcc aatatcaatg attttatttg
    12721 gacttggagg tacaacattt gggatggcag aagagacatt accattttat atgatatttg
    12781 ttccactcat gacatccttg ggatatgatt ctctaacagc aatagcaatt gtatttatag
    12841 gagcagcagc aggagcagcc gcttcaacaa taaatccatt ttcagttggt attgcacagg
    12901 cactagctca acttgctcct ggttcaggag taacttacag agtagttatt tttatagcat
    12961 atatactaat aagtatagca tttgttatgt actatgctaa gaaggtaaag gaaaatcctg
    13021 aaagttcaat agtatatgaa atagataaaa ataataaatc aatacttaat caaacttcaa
    13081 cagaaataaa agaatttact tttaaagaag tgtctgttct tgctatattt ggaataggga
    13141 tggcaataat gatttggggt gttttaagtc aaggatggta cattccagaa atatcaatgt
    13201 gttttgctgc aataggtgtg ctttctggat tggttggtag attatctcaa attgaaatct
    13261 cagaaagttt tatagatgga gcaaaagatt tagcatctgc agctcttgct atagctttgg
    13321 ctcgtggaat cgtaataatt gctcaaaatg gatttataat agatactata ttaaattccg
    13381 cagcagcctt tctaggtggg cttccaaagg ttatatttgt atatttatca tttttcttgg
    13441 aagctgcatt ggcattttta attccatcat cttcaggtct tgcttcactt acagtacctg
    13501 tacttgctcc tcttggtgat ttggttaata tatcggctca agtgatagta accgcttatc
    13561 aatttggtgt gggtcttaca aactttataa ctccaacttc aggagtactg atgggagcac
    13621 ttgcagtagc ccatattcca ttttcaaaat gggtaagatt tataatgcct atcttgttga
    13681 ttttgacagc tgtatctcta gtctttttaa caataggtat ttatataggg ttttaattaa
    13741 gtgtagtttt atttaagata tttagtaatg gtataaaatg ataatatgtt ttcaataaat
    13801 tgccactaat ttaattagtg gtaatttgtt ttttgtattt aaaaataaat gtatttactt
    13861 gtattttgaa ttaatatcta cttagacatt gttttaattg ttatattttt atttaattaa
    13921 gcattgattt atcatttttt atatttcata taacaatgca aaaaataatt tttatatagt
    13981 aaatgacaaa agtttacaat aaatgtatat tttcttactc attccttaaa attactcatt
    14041 tagtttaata actctcattt catatgtaaa gttggtaaaa aaattaaaat taaaagtaaa
    14101 aaactaaata aattaatgtt aatttgaaat atatatgaag gtttttggag aatacttact
    14161 tatgatagga aaaaatgtta aaataatagt gtaatcaaat ataaaggact gaaataatta
    14221 acagttatta aatattagga gtgtaaaaat ttatgggatt aaaagaattt gattttattg
    14281 aagagtctat tgatatgttg aggacaatgt caccaacatt agaaactata tcagatgaaa
    14341 tagaagagta ttttgaaaat atcttggatg aaaagaatca agaatatata aatgtaacct
    14401 ctagaataaa atcagaatca agtttaagag aaaagataat tagaaataga tatttaaaaa
    14461 aatatggaga agcaagtaat cttattcata atgtttctga cttaataggc ctaagaattg
    14521 agtgtagatt tattgaggat gaaaacaaaa tatatagact tttgagaaga tattttaata
    14581 aaacagatga caaaataaac tattataata aagagaataa gaatattaaa ttaaaactat
    14641 ctgagagaca acccaataag caaaaaaatg gatttgaaat atataaaata gatggagttt
    14701 ttgcgtattt agatagagaa gtaaaatttg aacttcaaat taaatctctt gtaaatgtgt
    14761 tttggagtga aatagaacat aaaataattt ataaaaacaa tacatattta cttgctgata
    14821 aatttataaa ggatatgatg gactctataa agaataatct tactatgata gataatcagc
    14881 ttttaagtat ttataaaaac tttcattcag ggaagtcttt taatatgaaa gtaagtaaaa
    14941 aagaaattga aaaattattt gcgaaattag tctatgacgc tttttctgaa aagatgaata
    15001 aaagtatagg ttttgttgta gattttaaaa aaccatgtga gactatattg aactattctt
    15061 ttaataagca agatataagt gatgaagttt taggtaattt tatgcttgat gagtttgcaa
    15121 gattaaatga aattgtaaac aaggatatag attttaatga gcaaatagaa tttgaaagag
    15181 aaccaacatt tgatgataag ttttgtaaag acctaggaaa tcattttaga agtagattaa
    15241 acacagaatt tccatggaac ctatttttta gaatattatt tgaaatagag ccatataaca
    15301 ataccaaaga ctttgaaaac tttgtagagt ttataaaaga aaatgtgcta ctagaagaaa
    15361 atagggagca attattatgc caatttgaag aagatagcaa gcttataatt gaagatatat
    15421 atgattgtat atttaatagt ataagtgaga ttgatagtgt tgaaatttta tataactata
    15481 atcttgaaaa aataaacttt acatcaagtg aaatcataaa ttgtatatgt agagaatatg
    15541 agtgttatgg tgaatactta gaagaaaaag aaaagcttat gaatacattt aaagaaaaga
    15601 ttattcaaat ctttgagtaa taggtcaatg ctgaagtggc gctaatgcgc catttttcaa
    15661 atatatatga attaagtaaa attattttgg aggaggcgat ttgtatagag aaaacaggtg
    15721 taattttggc agggggaaga aattctagaa tgggtagaga taaagccttt ttagagttac
    15781 atgataagct ttttattgaa attgcaatag aagcatttaa aaactttgat gagttgataa
    15841 taatatctaa taatgaagaa ttatattcta aatacgatat taaagtttat aatgatatag
    15901 taaaagatgt tggtccaatt ggaggaatat atactgctct tgaatatgca aagtatgata
    15961 tagttacaat tgcatgtgat atgccatatc taaataatca aatagtcgaa agaatagcta
    16021 ataaaatgga tgataaaagt gtgatttcag tgacaaatgg aaagttacaa ccattgtgtt
    16081 ctggatataa aaagagtata ataaataaag tatctttatg tataagggaa aatgatttaa
    16141 agctcagaag ctttatagat aaaatagata aaagttatat atactttgat gaagaagatt
    16201 tatttttaaa tgtaaacact gttgatgagt ataaaaaatt aactaaagtt taattagcta
    16261 tggttgtaaa atagagtagg atttatatgg aggagagttt atgaatttac ttaaatgcga
    16321 ttcttgggca gtaatattaa ataatagcga taaagaaagt aaagcatata aaatattaga
    16381 tgaacttaaa aggaatatgt acaaagttgt tgctatagat gaagagaaaa aacctattga
    16441 aggaatagat gtgtatgaat gtttaaaaga tgttccacac aatattgatg ttgtagctat
    16501 tattgataaa caatctaaga tggatgtgat tttagaagaa gtagagcttt tggatataca
    16561 aaatatgtgg tttgaaaaag gaagctttag tgaaatgatg attaaaaaga ctaaagattt
    16621 aaaattaaat atagaatata atttaagttt atatgatgag ttgactagat aaaattatat
    16681 ttcttaataa taaaaatata attagttaat aaaacatatt attttatagt atagattggt
    16741 taataaaagt acagattggt taatgaaaca ttaaattaca atatttgtta aaaaaattgt
    16801 gagttacatg atataatgta ttaaatagga tactaaggag ttgatatatt atgttaatag
    16861 taactacaga aaaagtagaa ggtaaaaaga tatcaaaggt tttaggatta gtgagaggaa
    16921 gtacaataag agcaaaacat gttggaaaag atataggagc aagttttaag aatcttgttg
    16981 gaggagaact tactggatat aatgaaatgc tcactgaagc aagacaaatt gccataggta
    17041 gaatggttga agatgcagaa gctaaaggtg caaacgcagt aatagcattt agactgtctt
    17101 cagcttcagt tatgcaaggg gcagcagaaa tgcttgctta tggaacagca gttgttttag
    17161 aagatgataa tagtattctt gaaaaataaa atataaatag ttaaaaagag gcatctttta
    17221 aaaattttat ggatgccttt ttttatttga aaaaactaat ttgagaagaa attttaacta
    17281 ttatgacaat aaaattaggg tgatattata taaataagta aaattatatt atataatgta
    17341 tggtatagac tttaaaaatt ttatttgtag agaaaaatat ggacaatgtt ataataatta
    17401 tagaaattaa aagtacttac attcattatg tatgtgacat gtataattag ttttatattt
    17461 aatacagttt tgaagtaata tcttcatttt gagaacgttg gaataataaa aaatgtatta
    17521 ttataggtga tataaataaa aaataaatat aatatgttat attgctgata gaaaagagaa
    17581 taaaagaaaa taggttttat taaaagagat ttttatttat aggacttaat caatgtatag
    17641 aggggtgaga acatatgttt aatgttgcaa ttataacact tagtgataaa ggatatgaag
    17701 gaaaaagaga agatataaca ggaaaaaagt taactgaatt tgttgaaaat acaggagctt
    17761 ataaagttac tgaatatgta cttataaaag atgataagga aatgctaaag gaaaatatta
    17821 taagactttg taatagtaat aaaatagatt taattcttac aaatggaggt actggttttt
    17881 caaaaagaga tataacccca gaagcaacta aggaagtgtt ggaaaaagaa ataccaggtc
    17941 tgagcgaata tatgagaatg aaatccacag aaattactaa aaaggccata ctaagtaggg
    18001 gtgtaagtgg aataagaaat aattctataa taataaattt acctggaagt cctaaaggtg
    18061 cagttgaaaa tctaagtttt ataattgatg ttttagacca tggcatagaa gttttaagag
    18121 gagaagctac agaatgtgcc actaaaaaaa gtgaatagta tataatctag atagataaaa
    18181 aatagacaaa gaggtagata aagtatgatt gataagtatg gaagaaaaat agactacctt
    18241 cgtatttcac ttacagataa gtgtaattta aggtgtgttt attgtatgcc accagatgta
    18301 aaatttgata aaaattatat aaatgaaaat ctaagctttg aagactacaa gtttattata
    18361 aaggctatgg ctgaacaagg tataacaaaa attagattta ctggaggaga acctctttta
    18421 tatcctaaat taaatgagct tataaaattt acaaaagaag aatgtggaat aaataacata
    18481 ggaatgacca ctaatgctat tgggctttct gatagaatct ttgagcttga aaaatgtggc
    18541 ttaaataaag ttaatataag tttagattct ttgaaggaat ataagtataa atccgtaaca
    18601 agaggaggaa acttaaaaga tgtcttaaaa tcgattgaat cttgtttaaa cttaggtata
    18661 aaagtaaagt taaattgcgt tgctataagt gggtttaatg atgatgagct taaagatttt
    18721 atgtttttaa cagttaaatc accaattgat gtaagattta tagagcttat gccattaggt
    18781 gaggctaata gagtttataa gaagggttat tttaatgtaa aagaaatgat tgaaaaaata
    18841 tatggtttat ataaagtcaa aaatgatgaa aaaagtacag cagaatatta tatgtttaaa
    18901 ggagctaaag gaagaatagg aattataact ccattaagct gttcattttg taacacttgt
    18961 aatcgtatta gacttacttc tagtggtgca ataaaattat gtctacattc aaaagaagaa
    19021 atagatataa aaccattttt acataaacca ttgttattta gagaatctat gaaagatata
    19081 ataaaacaaa aaccagaaaa acatcattta ctagaaaata aatgtagtga tacaaatagg
    19141 cgtatgtatg aaattggagg ataagtaatg ttaacacata taaatgaaaa agggtatgcc
    19201 aagatggtgg acataagtga aaaagatgat actaaaagaa cggccgtagc tacagctaca
    19261 attactctta gtaaggaagc attagaaaaa gtaaaaaatg gtcagataaa aaaaggagac
    19321 gtattgtctg tagctcaagt tgcaggtata atgggagcta aaaatacatc ttcaaacata
    19381 ccaatgtgcc atcagattaa attagttgga tgtgatttag aatttgatgt agatgattct
    19441 atgtctgcta ttaagatata cgcaacctgt aaatctcttg ggaaaactgg agtagaaatg
    19501 gaagctttaa atgcatgttc tttagcagca cttacaattt atgatatgtg taaagcagtt
    19561 gataaaaaaa tggttataag taatatccat cttgtggaga agactggtgg aaaatcagga
    19621 gattttaagt tcgagtaaat taataatata ttgggttaaa atttggagga aatatggcga
    19681 aggtagtgtc aataaatata agtgagaaaa agggtgtaat taaagttcct gtacaatgtg
    19741 cagagctaaa aataaatcat gggatagttg gagatgcaca tgcaggaaac tggcatcgtc
    19801 agataagctt acttggaaaa gaaagcatag ataaaatgaa aatgaaaggc tttgaccaat
    19861 taaaatatgg agattttgct gaaaatgtaa ctacagaagg gatagaagtt ttttccctgc
    19921 ctgttggtac aaagttaaga atagggcaat gtgaagttga agtaactcaa atagggaaaa
    19981 aatgtcacaa tggatgcgaa attaagaaat tgacaggtga ctgtgtgatg ccacgagaag
    20041 gaatttttgt aaaggttcta aaagaaggaa ttataaatgt gaatgattca atagatgtaa
    20101 tctaatgaag tcactattaa aatataatta taagtaaata ctattaagat ttaaatatat
    20161 attttaagag agaattaatt ttaataaaat attttagtta taaa
//
LOCUS       DAGSWI010000067        20168 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.067, whole genome shotgun sequence.
ACCESSION   DAGSWI010000067 DAGSWI010000000
VERSION     DAGSWI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.067"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            61..1020
                     /locus_tag="MJF80_002252"
     CDS             61..1020
                     /locus_tag="MJF80_002252"
                     /inference="COORDINATES: protein motif:HMM:NF025025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBZ0280910.1"
                     /translation="MNVSIIIPTKNAEKYIENLIKALINQTIEPCEIIVIDTESKDKT
                     VEICMEYDIVKFIQIKQSEFGHGSTRNRAAKLSKGDILVFMTQDALPQNCYFIEELIK
                     PLGKNNIVCTYGRQLPRNNAKTLEVFSRSFNYGNSDIIKSKEDIPKLGVKTFFFSNVC
                     SSFIKRDFFLVDGFPENIIMNEDMIISSKIIYNGKKTCYASKAIVTHSHRYTYLQQFK
                     RNFDIGVAFKENSYYLSEVKSESEGFKFVIEAIKFLFQSNKCYLIPSLFIESGFKFFG
                     YKFGMNYKNMPIKLVKLMSMHSFYFDRKEVMEEYYSENSNNRC"
     gene            995..1846
                     /gene="rfbD"
                     /locus_tag="MJF80_002253"
     CDS             995..1846
                     /gene="rfbD"
                     /locus_tag="MJF80_002253"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBZ0280911.1"
                     /translation="MKIVITGAKGQLGLEIVNQLDNLNNIREKNIYDIVPTDRSNLNI
                     TDLENVNNFIFKEMPDVVINCAAYTKVDLCENDIDNAYKVNALGVRNLATACEKIKAK
                     FVQISTDYVFDGNSNIPYKEDCITFPNSIYGKSKLMGEYFTRQFSSKYFIIRTAWLYG
                     DGDNFVRTMLNLAKNNNEINVVDDQIGSPTSTVELAEAIINLIETDYYGIYHGTCEGE
                     CSWYEFANKIFELSKITVKVNPITSNEFKREAPRPMYSVLDNFMLKLIGLNNFNRWDE
                     ALKKYLE"
     gene            1874..2752
                     /gene="rfbA"
                     /locus_tag="MJF80_002254"
     CDS             1874..2752
                     /gene="rfbA"
                     /locus_tag="MJF80_002254"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBZ0280912.1"
                     /translation="MKGIVLAGGSGTRLYPITKCVSKQVLPIYDKPMIYYPISVLMLS
                     GIRDILIISTVRDIKIFKELFGDGSQLGVNIQYAIQEKPNGIAEAFIIGEDFIGDDKV
                     ALVLGDNIFYGYGFSERLERAINRDEATIFGYHVSDPTSFGVVEFDKDFNVLSIEEKP
                     KIPKSNYAVPGLYFYDNEVIKIAKSIKPSKRGELEITSVNNEYLKKGKLKVELFGRGM
                     AWLDTGTHRGLLDASNYVEVVQTRQGLYIACLEEIAYIKGYITKEDLLELAKPLMKTD
                     YGKYLIKIANEFKENK"
     gene            2960..3856
                     /locus_tag="MJF80_002255"
     CDS             2960..3856
                     /locus_tag="MJF80_002255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280913.1"
                     /translation="MNSWRDTLYTPTSIEKIDDLYFIVDCWQHRVIYNDNLSDKISNW
                     KTLSDNLEGPHSIASDKEVFLVDNTEKNEIQVFKRKEDNFILTQTIQNVGKRPHKIIY
                     DKNTNLFYSIASTSQDIVVLENINGNVRVKQKTRLDFLDGSYIRSISIIEDKMYFLPG
                     PNKVICTDYKSGKFNVISEYKLPKALEAINHLYKIDNYYYISLYTMGENPNEPKLVRF
                     KNFNELQNNEYEDLTKKLNIKGVPYFFSEFDGKVFFTEIDSYSEIKSFKVNNDNIDSI
                     VNYYDSGKPSKESIERKKSILR"
     gene            3900..4472
                     /gene="rfbC"
                     /locus_tag="MJF80_002256"
     CDS             3900..4472
                     /gene="rfbC"
                     /locus_tag="MJF80_002256"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBZ0280914.1"
                     /translation="MSKFNFIETKLKDLYIIEPTIFGDSRGYFMETYNKREFLEAGLD
                     MEFVQENESKSKKGVLRGLHFQTENVQGKLVRVAFGEVYDVAVDLRIGSPTYGLWEGV
                     ILSSENKRQFYVPEGFAHGFLVLSNEAVFNYMCTNYYNADYEGGILWNDKDINIEWPL
                     DKVDSVILSEKDKKLLKLSELDLQFKYGGE"
     gene            4476..5498
                     /gene="rfbB"
                     /locus_tag="MJF80_002257"
     CDS             4476..5498
                     /gene="rfbB"
                     /locus_tag="MJF80_002257"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBZ0280915.1"
                     /translation="MSKVLITGGAGFIGGNFVHYILDKYPKYEVVNLDLLTYAGNLET
                     LENIKHKHNYKFVKGDISNRNFIFDLFDEERFDIVINFAAESHVDRSIKEPWKFINTN
                     VIGTQVLLDASNKYGVNRFHQISTDEVYGDLPLDRLDLLFTEKTNLNPSSPYSASKAS
                     ADLLTISYYKTYGLPVTISRCSNNYGPYHFPEKLIPLVIHRALENKELPIYGDGKNVR
                     DWLHVYDHCTAIDLIVHKGKSGNIYNVGGHNERSNLEVVKTILDTLGKSEDLISFVAD
                     RKGHDLRYAIDASKLEQELGWKSIYSFDEGIKHTIKWYLENQEWMESIISGEYKNYYS
                     NMYKNE"
     gene            5519..6307
                     /locus_tag="MJF80_002258"
     CDS             5519..6307
                     /locus_tag="MJF80_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280916.1"
                     /translation="MSLVKELYRNRDLILNLSKNDFKTKYAGSYLGIFWAFVQPVVIV
                     LIYWFVFQVGFKAAPVKDFPFILWLIAGIVPWFFFQEALINSTNSLLEYSYLVKKVVF
                     KISILPLVKIISALYVHIFFICFALASYCIYGIYPNIYIIQILYYSFCTMFLALGISY
                     ITASIIPFFKDLSQIISIFLQIGMWATPIMWSYNIMPTKYQWFLKLNPMYYIVEGYRD
                     TLVNNVWFWERYNQTLYFWTISIIIFFVGIILFKKLKPHFSDVL"
     gene            6321..7589
                     /locus_tag="MJF80_002259"
     CDS             6321..7589
                     /locus_tag="MJF80_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280917.1"
                     /translation="MNEIVIKVENISKVYKLYEKPIDRLKETIGLSKKVYHTKHYALE
                     NLSFQVEKGETIGIIGTNGSGKSTLLKMITGVLTPTSGNIIVNGKISALLELGAGFNP
                     EYTGIENIYLNGTMMGYKKEEMDDKIESILEFADIGEFINQPVKTYSSGMFSRLAFAV
                     AINVEPDILIVDEALSVGDVFFQAKCYKKFNDFKKQGRTIIFVSHDMGSIMKYCDKTL
                     LIDRGKFVDMGPSANMIDIYKKILVNQYVPDSDNEEIIEDVMEIEGTNKWYEKLQINP
                     TKLEYGNKKAEIIDFAIVDDKNKINNNINKGTIFNIKMKVKFNENIDNPIYAFTIKDL
                     KGTDVTGTNTMLEHIDTNKYDDKGEVIIVFKQKMDLQGGSYLLSLGCTGYESEDFTVY
                     NRLYDICNINVISLKNSVGYYDMNSEIEIY"
     gene            7617..8525
                     /locus_tag="MJF80_002260"
     CDS             7617..8525
                     /locus_tag="MJF80_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBZ0280918.1"
                     /translation="MEKYIGNIKLNFDFYKGNDLYSDGVVEDELLDIVKNYSEYEDII
                     KERKEWPIIYHLSKIRENIIEWIPINKTDNVLEVGSGCGAITGILSKKAKQVDCIELS
                     QKRSYINAYRNKSKSNINIFVGNFEDIEKNMTEKYDYITLIGVFEYAESYINSKKPYI
                     EFLRIVKKHLKKNGKIIIAIENRLGLKYWAGCKEDHLGTYFEGLEGYREDKGIKTFSK
                     NELEDIFKLVGFYKYNFYYPYPDYKLPITIYSDEYLPKLGELNNNFRNFDLDRVVTFN
                     ETEVFDSIIKNNLFPIFSNSYLIILE"
     gene            8544..9926
                     /locus_tag="MJF80_002261"
     CDS             8544..9926
                     /locus_tag="MJF80_002261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280919.1"
                     /translation="MSKIIYSKYSNERNKKFQIRTSILQNDDGKKIVKKLAIVDESKK
                     HIDDIFENFERLKDNYYLDERVKINLCNKTEDGLEFEYLEGMTLDKIIDDFMENNEYD
                     KSIEIIKDFRNVIFNVSTTCGFNITEDFIKVFGNVDFNSDTKATLLSNIDSSFNNLVI
                     NDAWNIIDYEWVYKFPIPIRYILYRALNIYITTSVYGSKLKDLNIYDILGYSELELSK
                     YEFMEYNFGRYIAGESISLQNLYEDIKTKKYKLEELLISNDFNKRMQIFYDEGNGFSE
                     ENSYYIDLEMKNKITIPLEKDIKSIRIDPASVNCIILINKLDVVFANETIDIKNNLIT
                     NADEKKQDLYTFLSSDPQIYIDLGNRFQKGYINFEYEIKKLDFDTYDIYLEVRNLMLN
                     NQNKLINLNKELEERNMQLDILGKEINQINDRLENTLNELDNAKNKIYILEKRKIKNI
                     LRNMKKRISK"
     gene            9960..11120
                     /locus_tag="MJF80_002262"
     CDS             9960..11120
                     /locus_tag="MJF80_002262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280920.1"
                     /translation="MHFFTSITTNYLPKARVLANSVKKNCENSWFILVVCDNLPNDFN
                     LEKEPFDEVWTIESLGLPVENLNMWIFQHTVVELCTAVKGQAMLNLLNKYEKCVYLDP
                     DMVVFDSLKELENLLDSHDIILTPHVTHKEEEEQHIRNNEIASLRYGAYNLGFLAVKR
                     NENGLEFVTWWRDRLCKYCFDDIPGGLFTDQKWIDLAPGLFEGVYILRDPSYNVATWN
                     ITWRKISNLDGKYKVNNIDLKIYHFSGFDSGAQEIMLKVNGGNNQALWNLREQYILEQ
                     EKNGQKELGSLKGKFDTFDNGEIISKDMRVLMRNRLDLQDYFKEKNPFCSVGEKSYYN
                     WYKNNVSFRLDDETLDEKSKKILELENELNRLRKMISPLQQIKKILKKFLKR"
     gene            11131..14679
                     /locus_tag="MJF80_002263"
     CDS             11131..14679
                     /locus_tag="MJF80_002263"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF025665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HBZ0280921.1"
                     /translation="MNNRDKNILFLIHDYIKYDTPMNVGGTQLHIEDLISKIENETFY
                     ILYKKKNKLILDEYRDSKLINTYNYYCGDLSSVVEYTNPLFKLIYSAILVNFKIDLIH
                     IHHLKNHTFDIIYEAKSLNLPIIITIHDYYLVCPKINLIDRNNCCCVNRETKELCGEC
                     MSEEIGMSFEELHLYREEVSKILLMCNEVIIPNKSVSEVLLIHYYCIKEKIRVIEHGQ
                     TGEVSKYAPTYKNGKFNVAFVGGLKPCKGSKEIIKIIKNCTDKDINFFVFGTIDDKDF
                     DDIPSHNINCTGNYFRKDIYKLLEENEIHIVCILSIWPETFCYTLSESWQCNIPVITV
                     DIGATGERVKNGGGILITGNNIGESTLKSIIELKNDRNKYESLCKTVKNIKLRDIGEM
                     SKDYSNLYFSYMKNVMLDNKISSELILDALKNIDLFQTNNSIKNYELEDLKNEIKYIK
                     GTFAYRVWQKYKDMKIPFKSNISSFFKRKVSLEKDISKNKNIIKRKSEQLNIISNIEY
                     EQVYYENEKLNFYVEGWFFSKYYDMNVSCENCESFSIERLSRPDVQSHFGGKKEAENS
                     GYRIAFIGVEENSKISLVFDIGNLKKYYNFNVDTSQGVVYIGKYPKKLRWKSIPQLFD
                     MTVVEPYKYKKVIDKNSETIENVSLILNATRPIKKEWAEETIRSIINQGFKSLYIISC
                     NENLNLFSEIDNIMLVSSKLLTRKEECLEFLTSKYDFNCEYVSFISIGDKMEVNTIRE
                     MIKEAKKSNSFIVTTNENRFFEEEYFAPFYKQQLYNRFDNPSIDRLCRKSMLISTDKI
                     IDFISGNIKSNEIKIIDKILYNYRLYNDNIDNKVKVLAYYLPQFHTIPENDEWWGEGF
                     TEWVNVNRGRPMFKGHYQPHVPGELGYYNLVKDEGIQKKQSQLANDYNIYGMCYYYYW
                     FNGKKLLEKPLENLIADKSINTNFCICWANENWCRRWDGLENEILIKQEHNVQSDKRF
                     IYDVLPILKDERYITIDNKPILLVYKSDLFPNFISTVESWNKICIENGLEGLEVITVQ
                     TGGLEDPRNMGCKYAVEFPPHNIYSRVLSAKSKVEELDKDFEGTILDYSEVAGIGMNK
                     KAQPYTFFRGVMLSWDNTARRNTQSRIFYGSNPNEYKKWIASVIDFSINFYEEKERMI
                     FINAWNEWAEGTHLEPDEKYGHKYLEVTKEVLGAQY"
     gene            14857..16227
                     /locus_tag="MJF80_002264"
     CDS             14857..16227
                     /locus_tag="MJF80_002264"
                     /inference="COORDINATES: protein motif:HMM:NF025665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HBZ0280922.1"
                     /translation="MNKLNSLIKRLYHMLPLSYEKKILLKNNVYKLLSPFIKNTNMYS
                     IWINSLENPEKYIKDNNPKKGVDNEKDIYIDNLFKRASEKSSNYIEISDTRFQLEKND
                     IKLISFYLPQFHPIVENDEWWGRGFTEWTNVSKAVPQFLNHYQPHLPAELGFYDLRLK
                     EVMKRQIELAKMYGIYGFCFHHYWFAGKRLLERPVNMLLKDKELDIPFCLCWANENWT
                     RRWDGLENEVLIEQKHSKEDDLEFIKDISNYFKDERYIKIDGKPVLIIYRIELLPNPI
                     ETIKLWRKYCLEIGWNDIFIVGAQTFGFEEHIKYGMDAGVEFPPHNINNCPILNKEIN
                     FTNKKFTGLVYDYKKLVDDKLYIKKSHGKVFKTVMPSWDNTARKPNNASIFKNSTPEL
                     YKKWLKDIIYSTKENVLDSDNFIFINAWNEWGEGAHLEPDRKYGYSYLNATREAIIET
                     RSEKNN"
     gene            16268..18064
                     /locus_tag="MJF80_002265"
     CDS             16268..18064
                     /locus_tag="MJF80_002265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280923.1"
                     /translation="MDKLKKNNNLKIKIIFFILLQIFFIFFLVVNFKTPLLGEDYALL
                     YNGPFKDIGDYFSTIMAALEKQMSTWMVRMGNPLTIIWLSFDKSIYNIAVSFVSMIFF
                     FLIFFYGNARKPNVNNLGDLIGFFVPASLMMFLSPSIGDLFFWCNGSLNYLWPLCILL
                     LCAIPYRLYLSEKNINIKNPILIILYCIACFISGFTNENTPPVIITLVAALIIRDLYL
                     KKKPPKWIVFSFISICAGWIGLVVNSTTRFRSMYYRNMFGLPEHATINELINNVLRVG
                     YLFLKSGFAFVIIFLILSLLFIFILKSQKTENEFKYKHLFAENMFFILLTFISAAALV
                     FAPYTELRSFTLIHTIILATISNLLVNLYEISSHKQRLGLSSISIITLLSTFLLFINI
                     YNEYNDYYKFDTQRTCDIKSQVAAGKTTVYASKNFIENRTMNNREFYIYGNDLGQGGN
                     FYSDYFGAKKIIWYDKIDSNIDPATINLSSESTDVNVELINGNSEQDNSSLLSDGIFN
                     VSGWAIDKVAGNSPSKVFIKIDDKYYQARTVIREDVAKYFDNKKYKKAGFECSIPTKD
                     IKKGKHKVTLCVISNDGEKRYEVDSGKSIEFK"
     gene            18095..18985
                     /locus_tag="MJF80_002266"
     CDS             18095..18985
                     /locus_tag="MJF80_002266"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBZ0280924.1"
                     /translation="MASLIKQRKYNRNILLEYIKLMRPKQYIKNGFVLAALIFSNNIL
                     NPSLALKSILSFIAFCMISSSAYILNDILDIEKDKMHPKKCKRPLASGSIGKKGAISL
                     GIVLVLSSILLSLLIHKNLCVIILLYLFNNIMYSLKLKNIILIDVFSIAIGFILRVCA
                     GSIAINVSLSSWIILCTFFLSLYLGFGKRKKEIILLKEDASNHRKILKEYDEENLNQM
                     MSISLSATIVCYSLYSANNIYNSNMIFTTVFVVYGVLRYNYIVNIGDEGNPTDVVLND
                     KSLKFCVLFWVIACIGILST"
     gene            19032..20006
                     /locus_tag="MJF80_002267"
     CDS             19032..20006
                     /locus_tag="MJF80_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBZ0280925.1"
                     /translation="MFMINIVIPNYNGYKLLKNCLDSLKNQTYSNFSIIVVDNASDDS
                     SYNWLNNYENVTLKKLDKNYGFSKAVNEGIKLSNSKYVVLLNNDTVVPENWLETLLGE
                     IEKDESIFSVCSKMIRYNEKDKIDDAGDEYSILGWAYKIGDGKNISSFRKSKYVFSSC
                     AGAAIYRNSIFDEIGYFDEDFFAYMEDIDISYRAKIYGYKNRYCSEAKIFHIGSATSG
                     SKYNSFKVRLAARNNVYVPYKNMPFLQLIINMPFLIIGFLVKYLFFLKKGFGNDYKEG
                     FFEGIKNLKNLNKTEFRIKNLFNYLIIEALLILNMCKYIFSKVLDIYN"
BASE COUNT         8141 a         1835 c         3347 g         6845 t
ORIGIN      
        1 aaatcaaaat ctaaagtaaa atgtgtagaa tcattaaata aatcaaaagg ggatgatagt
       61 ttgaatgtga gtataataat accaactaag aatgcagaaa agtatataga gaatcttata
      121 aaagcactaa taaatcaaac tatagaacct tgtgagataa tagttataga tactgaatcg
      181 aaagataaaa ctgtagaaat ttgtatggaa tacgatattg tcaaatttat acaaataaaa
      241 caaagtgaat ttggtcatgg ttcaactaga aatagagctg ctaaattaag taaaggtgat
      301 attctagttt ttatgacaca agatgctttg ccacaaaatt gttattttat agaggaacta
      361 ataaaacctt taggtaaaaa taatattgta tgtacttatg gaagacaatt gccaagaaat
      421 aatgcaaaaa cactagaagt attttctaga agctttaatt atggaaatag tgatataata
      481 aaatctaaag aagatattcc taaacttgga gtaaagactt ttttctttag taatgtttgt
      541 tcttctttta taaaaagaga cttttttttg gttgatggat ttccagaaaa tataataatg
      601 aatgaagata tgataatatc ttctaagatt atatataatg gtaaaaaaac ttgttatgca
      661 tctaaggcta ttgttacaca ttcacataga tatacatact tacaacaatt taaaagaaac
      721 ttcgatatag gtgttgcatt taaagaaaat agttattatt taagtgaagt aaagtctgag
      781 tcagaggggt ttaagtttgt catagaagcc attaagtttt tatttcaaag taataaatgt
      841 tatttgattc ctagcttgtt catagaaagt ggatttaaat tttttggata taaatttggt
      901 atgaattata aaaatatgcc tataaaatta gttaaattga tgagcatgca ttctttttat
      961 tttgatagaa aagaagttat ggaggagtat tatagtgaaa atagtaataa caggtgctaa
     1021 gggacaatta ggtttagaga tagtaaatca attggataat ttgaataata taagagaaaa
     1081 aaatatatat gatatagttc caacagatag aagtaactta aatattactg acctagaaaa
     1141 tgttaataat tttattttta aagagatgcc agatgtcgtt ataaattgtg ctgcttatac
     1201 caaggtagat ttatgtgaaa atgatataga taatgcatac aaagtaaatg ccttaggtgt
     1261 tagaaatttg gctacagcct gtgaaaaaat aaaagctaaa tttgttcaaa tatctactga
     1321 ttatgtattt gatggaaata gcaatatacc ttataaggaa gactgtatta cttttccaaa
     1381 ttctatatat ggaaagagta aattaatggg tgagtatttt acaagacaat tttcttcaaa
     1441 atattttata attagaacag cttggttata tggagatggt gataactttg ttagaaccat
     1501 gcttaattta gcaaaaaata ataatgaaat taatgttgtt gatgatcaga ttggaagtcc
     1561 aactagtact gttgaattag ctgaagctat aattaatttg attgaaacag attattatgg
     1621 aatataccat ggtacctgtg aaggtgaatg tagttggtat gaatttgcca acaaaatatt
     1681 tgagttaagt aaaattactg ttaaagttaa tccaataaca agtaatgaat tcaaaagaga
     1741 agcacctaga ccaatgtatt cagttttaga taattttatg ttaaaattaa ttggattaaa
     1801 taattttaat agatgggatg aagctttaaa aaagtattta gaataaaaaa taaatttgtt
     1861 tggaggataa attatgaaag gaattgtgct agcaggcgga tctgggacta ggttatatcc
     1921 tataactaaa tgtgtgtcta aacaagtact accaatatat gataagccaa tgatttatta
     1981 tcccatatca gtgcttatgc tttcaggtat aagagatata ctaataattt ctactgtcag
     2041 agatataaaa atttttaaag aattgtttgg agatggatct caattaggag ttaatataca
     2101 atacgctatt caagaaaaac caaatggtat tgcagaagca tttattatag gagaggactt
     2161 cattggagat gataaagtag ctcttgtttt aggagataat atattttatg gttatggatt
     2221 tagtgaacga ttagagagag ctattaatag agatgaagct actatatttg gatatcatgt
     2281 aagtgacccg acatcttttg gtgtagtaga atttgacaaa gattttaatg tactatcaat
     2341 agaggagaaa cctaaaatac ctaagtctaa ctatgctgtt ccaggtcttt acttttatga
     2401 taatgaagtt ataaagattg ctaagagtat aaaaccttct aaaagaggag aattagaaat
     2461 aacttctgta aataatgagt atttaaaaaa gggaaagctt aaagtagaac tttttggacg
     2521 tggtatggct tggctagata ctggcacaca tagagggcta ttagatgcat ctaattatgt
     2581 agaagttgtt caaacaagac aagggttata tattgcttgt ttagaagaaa ttgcttatat
     2641 taagggatat ataacaaaag aagatttact agaacttgct aagccattaa tgaaaaccga
     2701 ttatggtaaa tatttgataa aaatagctaa tgaatttaaa gaaaataaat gaaaataaga
     2761 taattattaa aactgtatga atactataat ataaaaatga caacttagtt tatataatat
     2821 ttgattgtat aaattaagtt aaattatgta ctaaatattt aaacaataag aaggtgtaat
     2881 tttgaatatt aaaaaaaaat ttaaaatttg tattctactt atacttgtat gtatgttatt
     2941 tttaggagct tggtctaaaa tgaattcttg gagagacaca ttatatacac ctacatcaat
     3001 agaaaaaata gatgatttat atttcatagt agattgttgg caacaccgtg ttatatataa
     3061 tgacaatctt agtgacaaaa tatctaactg gaagacttta agtgacaatt tagaaggtcc
     3121 tcattctatt gcaagtgata aagaagtatt tctggtagat aatacagaaa agaatgaaat
     3181 ccaagttttt aaaagaaaag aagataattt tatattaact caaactattc aaaatgttgg
     3241 gaaaagacca cataaaatca tatatgataa gaatacaaat ttattttaca gtattgctag
     3301 tacctcacaa gatatagtag ttcttgaaaa tatcaatggg aatgtaagag ttaaacaaaa
     3361 aactaggtta gattttcttg atggatctta cataaggtca atttctatta ttgaagataa
     3421 aatgtatttt ttaccaggtc ctaataaggt gatttgtaca gactataaaa gtggaaaatt
     3481 taatgtgatt tctgaatata agcttccaaa agctttagaa gcaataaatc atctttataa
     3541 aatagataat tattattaca tatcacttta cacaatgggt gaaaatccta atgaacctaa
     3601 attagttaga tttaaaaatt ttaatgaact tcaaaataat gaatatgaag atcttacaaa
     3661 aaaactaaat attaaaggag taccttactt tttttcagag tttgatggga aagtattttt
     3721 tacagaaata gactcttatt cagagattaa atcatttaaa gtaaataatg ataatataga
     3781 tagtattgtg aattattatg attcaggcaa accatcaaaa gaatctattg aaagaaagaa
     3841 aagtatttta agatagatat caatgattaa atattattgt ataataaagg agaaatacta
     3901 tgagtaagtt taattttata gaaacaaaat taaaagattt gtatataata gaacctacaa
     3961 tatttggaga tagtagagga tattttatgg aaacctacaa taaaagagag tttttagagg
     4021 ctggacttga tatggagttt gttcaggaaa atgaatctaa atctaaaaaa ggtgttttaa
     4081 gaggattaca ttttcaaact gaaaatgttc aaggaaaact agttagagta gcttttggag
     4141 aagtgtatga tgtagctgtt gatttaagaa ttggttctcc aacttatgga ctatgggaag
     4201 gtgttatact atcatcagaa aataaaagac aattttatgt accagaaggt tttgcacatg
     4261 gatttttagt actatctaac gaagctgtct ttaattatat gtgtactaat tattataatg
     4321 ctgattatga aggtggcata ttatggaatg ataaagatat aaatatagaa tggcctttag
     4381 ataaagtaga cagcgttata ttatctgaaa aagataaaaa actattaaag ttaagtgaat
     4441 tagatttaca atttaaatat ggaggtgagt agaaaatgag taaagtttta attactggag
     4501 gcgcgggatt tattggtgga aattttgttc attatatctt agataaatat cctaaatatg
     4561 aagttgtaaa tttggatttg ttaacttatg ctggtaactt agaaacatta gaaaatataa
     4621 aacataaaca taattataaa tttgtaaagg gtgatatttc aaataggaat tttatatttg
     4681 atttatttga tgaagaaaga tttgatatag tcataaattt tgcggctgaa tcacatgtgg
     4741 ataggtctat caaagaaccg tggaaattca ttaatacaaa tgttattgga acacaagtac
     4801 tgctagatgc ttctaataaa tatggtgtaa atagatttca tcaaatttcc actgatgaag
     4861 tttatggaga tttgccactt gatagattgg atttactttt tactgaaaaa actaacttaa
     4921 atccaagttc accatattct gcttctaaag catcagcaga tttattgact atttcatatt
     4981 ataagacata tggattacct gttactatat caagatgttc aaataattat ggaccatatc
     5041 attttcctga aaaactaata cctctcgtaa ttcatagagc attagagaat aaagagttac
     5101 ctatttatgg agatggaaaa aatgtaagag actggttgca tgtatatgat cattgtacag
     5161 caattgattt aatagttcat aaaggtaaaa gtggaaatat ttataatgta ggtggacaca
     5221 atgaaaggtc taatttagaa gtagttaaaa ctattttaga tactttaggt aaatctgaag
     5281 atttaatatc tttcgttgca gatagaaaag ggcatgattt aagatatgct atagatgcaa
     5341 gtaaattaga gcaagaatta ggatggaaaa gtatatatag ttttgatgaa ggtataaaac
     5401 atacaataaa gtggtattta gaaaatcagg aatggatgga aagcataata tctggagagt
     5461 ataaaaatta ttatagtaat atgtataaaa acgaataata ttgattggag aaattaaaat
     5521 gtcattagta aaagaattat ataggaatag agacctgata ttaaacttat ctaaaaatga
     5581 ttttaaaact aagtatgcag gttcatatct tggaatattt tgggcatttg tacaaccagt
     5641 agttatagtt cttatatatt ggtttgtttt tcaagttggt tttaaagcgg cacctgtaaa
     5701 agattttcct ttcatattat ggttaattgc aggaatagtc ccttggttct tttttcaaga
     5761 agcactcata aattccacaa atagtttgct tgagtatagt tatttggtta aaaaagttgt
     5821 atttaaaata agtatactac cattagtaaa aatcatatct gctttatatg tacacatatt
     5881 ttttatttgt tttgcactag cttcttattg catatatggt atatatccaa atatctatat
     5941 aattcaaata ttatattatt ctttttgtac tatgttttta gctcttggga tatcatacat
     6001 aacagcttct ataattcctt tttttaaaga tttaagtcaa attataagta tatttttaca
     6061 aattggaatg tgggctacac caataatgtg gagttataat ataatgccaa caaaatatca
     6121 atggttttta aaattaaatc ctatgtatta tattgtggaa ggttatagag atacattagt
     6181 aaataatgtt tggttttggg aacgttataa tcaaacatta tacttctgga ctatatctat
     6241 aatcatattt tttgttggaa taatattatt taagaaatta aaaccgcatt tttcagatgt
     6301 gctgtagaag aggtatacat atgaatgaga ttgttataaa agttgaaaat ataagtaaag
     6361 tgtataaatt atatgaaaag ccaatagata gactaaaaga gactataggg ttatcaaaaa
     6421 aagtatatca tacaaagcat tatgcattag aaaatctttc gtttcaagtt gaaaaaggag
     6481 aaactatagg catcataggt acaaatggtt ccggaaaatc tactttatta aagatgataa
     6541 caggagtatt aactccaaca agtggtaata taattgtcaa tgggaagata tcagccttat
     6601 tggaattagg agctggattt aatcctgaat atacaggtat agaaaatatc tacttaaatg
     6661 gtacaatgat gggatataaa aaagaagaaa tggatgataa aatagaatca atactagaat
     6721 ttgcggatat tggtgaattt attaatcaac ctgttaaaac ttattcaagt ggtatgtttt
     6781 ctagattggc ttttgcagta gcaattaatg ttgaaccaga tatacttata gtagatgaag
     6841 cacttagcgt aggagatgtt ttttttcaag caaaatgcta taaaaaattt aatgatttca
     6901 aaaaacaggg tagaactatt atatttgtaa gtcatgatat gggaagtata atgaaatatt
     6961 gtgacaagac attgcttatt gataggggca aatttgttga tatgggccca tcagcgaata
     7021 tgattgatat atataaaaaa atattagtga atcagtatgt tcctgatagt gataatgaag
     7081 aaattattga agatgttatg gagattgaag gtacaaataa atggtatgaa aaattacaaa
     7141 tcaatcctac aaaacttgaa tatggaaata aaaaagcaga aataattgat tttgctattg
     7201 tagatgataa aaataaaatt aataataata taaataaagg tacaatattt aatataaaaa
     7261 tgaaagttaa atttaatgaa aatatagata atccaattta tgcattcaca ataaaggatt
     7321 tgaaaggtac tgatgtaaca ggaacaaata caatgttgga acatattgat acaaataaat
     7381 atgacgataa gggtgaagtt ataatagtat ttaagcaaaa aatggattta caaggaggtt
     7441 catatttatt atcattagga tgcactggat atgaaagtga agattttaca gtatataaca
     7501 ggttatatga tatatgtaat ataaatgtaa tatcattaaa aaatagtgtt gggtactacg
     7561 atatgaattc tgaaatcgaa atttattaag ttataattga aaggtagttt tagaatatgg
     7621 aaaaatatat aggtaatata aaattaaatt ttgattttta taaaggaaat gatttatatt
     7681 ctgatggagt agttgaagat gaacttttgg atattgtaaa aaattatagt gaatatgaag
     7741 atattataaa agaaagaaaa gaatggccaa ttatatatca tttatctaaa ataagagaaa
     7801 atattattga atggattcct atcaataaaa cagataatgt tttagaagta ggttcagggt
     7861 gtggtgcaat tacaggaata ttatcaaaaa aagcaaaaca agttgattgt atagaattat
     7921 ctcaaaaaag aagttatatc aatgcatata ggaataaaag taaaagtaat ataaatatat
     7981 ttgttggaaa ttttgaagat atagaaaaaa atatgactga aaagtatgat tatataactt
     8041 taataggagt atttgagtat gcagagagtt atattaattc taaaaaacca tatattgaat
     8101 ttcttagaat agtaaagaag catctaaaga aaaatggtaa aattattatt gcaatagaaa
     8161 ataggttagg attaaaatat tgggctggat gcaaagaaga tcatttagga acatattttg
     8221 aaggtttaga aggatataga gaagataaag gtataaaaac cttctctaaa aatgaacttg
     8281 aagatatatt taagttagtt ggtttttata agtacaactt ttattatcca tatccagact
     8341 acaaattacc aataacaatc tattcagatg aatatctacc taaattagga gaacttaata
     8401 ataattttag aaattttgat ttagatagag tagttacatt taatgaaact gaagtatttg
     8461 attctattat taagaataac ttatttccta ttttttcaaa ttcttattta ataatattag
     8521 aataaaagga agtgaaaatc cagatgagta aaattattta cagtaaatat tcaaatgaaa
     8581 gaaataaaaa atttcaaata aggacaagta tattacaaaa tgatgatgga aaaaaaattg
     8641 taaaaaaatt ggctattgta gatgaatcta aaaaacatat tgatgatatt tttgagaact
     8701 ttgagagatt aaaggataat tattatctgg atgaaagagt aaaaataaat ctatgtaata
     8761 agactgagga tggtttagag tttgagtatt tggaaggtat gacattagac aaaataatag
     8821 atgattttat ggaaaataat gaatatgata agtctataga aattattaaa gattttagaa
     8881 atgtcatttt taatgtgagt actacttgtg gttttaatat tactgaagat tttataaaag
     8941 tatttggtaa tgtagatttc aattctgaca caaaggccac actattaagc aacatagatt
     9001 cttcctttaa taatcttgtt attaatgatg cttggaatat aattgattat gaatgggtat
     9061 ataaattccc tataccaata agatatattt tatacagagc tttaaatatt tatataacta
     9121 catcagttta tggaagtaag ctaaaagatt taaatattta tgacatatta ggatatagtg
     9181 agttagaatt atctaagtat gagtttatgg aatataattt tggacgttat attgctggtg
     9241 aatctatatc attacaaaat ttatatgaag atataaaaac aaaaaagtat aagttagaag
     9301 agttattgat tagtaatgat tttaataaaa ggatgcaaat attctatgat gaaggcaatg
     9361 gctttagtga agaaaactca tattacattg atttagaaat gaagaataaa attacaattc
     9421 cattagaaaa agatattaaa tctattagaa tagaccctgc ttctgttaat tgtattattc
     9481 ttataaataa attggatgta gtttttgcaa atgaaactat agatataaaa aataatttaa
     9541 ttactaatgc agatgaaaaa aaacaggatt tatatacttt tcttagcagt gaccctcaaa
     9601 tatatataga cttagggaat aggtttcaaa aaggctatat aaactttgaa tatgagatta
     9661 aaaaacttga ttttgataca tatgatattt atttagaggt cagaaattta atgttaaata
     9721 atcaaaataa gttaataaat ctaaacaaag aacttgaaga aagaaatatg caattagata
     9781 tactaggtaa agaaatcaat caaataaatg atagattaga aaatacacta aatgaactgg
     9841 ataatgcaaa aaataaaatt tatatattag agaaaagaaa aataaagaat atattgagaa
     9901 atatgaaaaa aagaattagt aaataattat tttatttcaa aagaaggagt attaaaaata
     9961 tgcatttttt tacaagtatt actacaaact atttgccaaa agctagagta ttagcaaatt
    10021 ctgtaaagaa gaattgtgaa aactcatggt ttattttagt tgtatgtgat aatctaccaa
    10081 atgattttaa tttggagaaa gaaccttttg atgaggtttg gactattgag agtttaggac
    10141 tacctgttga aaatttaaat atgtggattt ttcaacatac agtagtagaa ttgtgtactg
    10201 ccgtcaaagg gcaagccatg cttaatttat taaataaata cgaaaagtgt gtgtatttag
    10261 atcctgatat ggtagtattt gatagtttga aggaacttga aaatctttta gacagtcatg
    10321 atataatatt aacaccacat gtaactcata aagaagaaga agaacaacat attcgtaata
    10381 atgaaattgc atcactacgt tatggtgcat ataatttagg atttttggct gtaaaaagaa
    10441 atgaaaatgg tttagagttt gttacatggt ggagagatag actttgtaaa tattgctttg
    10501 atgatatacc aggaggactt tttactgacc aaaaatggat agatcttgca ccagggctat
    10561 ttgagggggt atatatatta cgagatcctt catataatgt agctacttgg aatattacat
    10621 ggagaaaaat cagtaatttg gatggaaaat acaaagttaa taacattgat ttaaaaatat
    10681 atcacttttc aggttttgat tctggagcac aagagataat gttgaaagta aatggaggaa
    10741 ataatcaagc gttatggaat ctaagagaac agtatatttt agaacaagaa aagaatggtc
    10801 aaaaagagtt aggaagttta aaaggtaaat ttgatacatt tgataatgga gaaatcatat
    10861 caaaagatat gagagtattg atgagaaata gattagattt acaagattat tttaaggaaa
    10921 agaatccatt ctgctctgtt ggagaaaaaa gttattataa ttggtataaa aataatgtat
    10981 cttttaggct tgatgatgaa actttagatg aaaaaagtaa aaaaatttta gaacttgaaa
    11041 atgaactaaa tcggctaaga aaaatgataa gcccactaca acaaataaaa aaaatcttga
    11101 aaaaattttt aaagagatag gaggaaaatc ttgaataata gagataaaaa tattttgttt
    11161 ttgatacatg attacataaa atatgatact cctatgaatg taggcggaac ccagttacat
    11221 atagaagatt taatttcaaa aatagaaaat gagacatttt atattttata taagaaaaaa
    11281 aataaattaa tcttagatga atatagagat tcaaagttaa taaatacata taattattat
    11341 tgtggagatt tatcttcagt agtagaatac acaaatccgt tatttaaact tatatactct
    11401 gctatacttg tcaacttcaa aatagatttg attcatatac atcatttaaa aaatcacaca
    11461 tttgatataa tatatgaagc taaaagttta aacttaccaa ttattattac tatacatgat
    11521 tattatttag tatgccctaa gattaatctg attgatagaa ataattgttg ttgcgtaaat
    11581 agagagacta aagaactctg tggagaatgt atgtctgaag aaataggtat gtcatttgaa
    11641 gaactacatt tgtatagaga ggaagtaagt aaaatattat tgatgtgcaa tgaggttatt
    11701 ataccaaata aatctgttag tgaagtactt ttaattcact attattgcat aaaagaaaag
    11761 ataagagtaa tagaacatgg acaaacaggt gaagtatcta aatacgcacc tacatataaa
    11821 aatggaaagt ttaatgttgc atttgtagga ggattgaaac catgcaaagg tagtaaagaa
    11881 attataaaaa taattaaaaa ttgtactgat aaagatatta atttttttgt atttgggaca
    11941 atagatgata aagattttga tgatattcca agtcataata taaattgtac aggaaactat
    12001 tttagaaaag atatatataa attattagaa gaaaatgaaa tacacattgt ttgtatatta
    12061 tcaatttggc ctgaaacttt ttgttacact ttaagtgaat cttggcaatg taatatacct
    12121 gtgattacag ttgatattgg agctacagga gagagagtga aaaatggtgg aggtatttta
    12181 attactggaa ataatattgg agaaagtact ctaaaatcaa ttatagagtt aaagaatgat
    12241 agaaataaat atgaaagttt gtgtaagaca gttaaaaaca taaaactacg agatattgga
    12301 gaaatgtcta aagattattc taacctatat tttagttata tgaaaaatgt tatgttagat
    12361 aataaaattt cttcagagct aatattagat gctttaaaaa atatagattt atttcaaaca
    12421 aataacagta ttaaaaatta tgaacttgaa gatctaaaaa atgaaattaa gtatataaaa
    12481 ggtacttttg cctatcgtgt ttggcaaaaa tacaaggata tgaagatacc ttttaagagt
    12541 aatatatcta gtttctttaa aagaaaagta tctttagaaa aagatatatc aaaaaataaa
    12601 aatattataa aaagaaagtc tgaacaattg aatataatat caaatattga atatgaacaa
    12661 gtttattatg aaaatgaaaa gttaaacttt tatgttgaag gttggttttt cagtaaatat
    12721 tatgatatga atgtatcttg cgaaaattgt gaaagtttta gtatagaaag gttaagtaga
    12781 ccagatgtac aatctcattt tggtgggaaa aaagaagctg aaaatagtgg atatagaatt
    12841 gcttttatag gggtagagga aaatagcaaa atatcactgg tttttgatat aggtaatttg
    12901 aagaaatatt ataattttaa tgttgatact tcacagggag ttgtatatat tggaaaatat
    12961 cctaaaaaac ttagatggaa gagtatacca caattatttg atatgacagt agtagagcct
    13021 tataagtata aaaaggtaat agataaaaat agtgaaacaa ttgaaaatgt ttctctgata
    13081 ttaaatgcaa ctcgtccaat aaaaaaagag tgggctgaag aaaccattag aagtataata
    13141 aatcaaggat ttaaatcctt atatataatt tcttgtaatg aaaatttaaa cttattttca
    13201 gaaatagata atattatgct agtgagttct aaattgttaa ccagaaaaga agagtgtttg
    13261 gagttcttaa ctagtaaata tgattttaat tgtgagtatg tttcttttat atctattggg
    13321 gataagatgg aagttaatac aattagagaa atgataaagg aagcaaaaaa aagtaatagt
    13381 tttatagtaa ctacaaatga aaatagattt tttgaagaag aatattttgc tccattttac
    13441 aaacaacaac tttataatag atttgacaat ccatcaatag atagattatg tagaaagagt
    13501 atgttaattt ctactgataa aataattgat tttattagtg gaaatataaa aagtaatgag
    13561 attaagatta ttgacaagat attgtataat tatagattat ataatgataa catagataat
    13621 aaagtaaaag tattggcata ttatttacca caatttcata ctattcctga aaatgatgag
    13681 tggtggggag agggatttac agaatgggta aatgtaaatc gtggacgacc tatgttcaaa
    13741 ggtcattatc aaccacatgt tccaggggag ttaggctatt acaatttagt aaaagatgaa
    13801 ggaattcaga agaagcaatc tcagctagca aatgattata atatatatgg aatgtgctat
    13861 tattactatt ggtttaatgg taaaaaatta ttggagaaac cgttagaaaa tcttatagct
    13921 gataagagta taaatacaaa tttttgtatt tgttgggcaa atgaaaattg gtgtagaaga
    13981 tgggatggac ttgaaaatga aatacttata aaacaagaac ataatgttca gagtgataaa
    14041 agatttattt atgatgtatt accgatacta aaggatgaac gttatataac tatagataac
    14101 aaaccgattt tactagttta taagtcagac ttattcccaa attttatatc aactgttgaa
    14161 agttggaata aaatttgtat agagaatggt ctagagggtc tagaagtaat tactgttcaa
    14221 actggaggtc tagaggatcc aagaaacatg ggctgtaaat atgcagttga atttccacca
    14281 cataatattt attcaagagt cttatctgca aaatcaaagg tagaagaatt agataaagat
    14341 tttgaaggaa ctatattaga ctatagtgag gttgcaggca taggaatgaa taaaaaagct
    14401 cagccatata cattttttag aggtgtaatg ctaagttggg ataatacagc acgacgaaat
    14461 acacaatctc gtatttttta tggctcaaat ccaaatgaat ataaaaaatg gattgcatcc
    14521 gtaattgact tttcaataaa tttttatgaa gaaaaagaac gtatgatttt tataaatgca
    14581 tggaatgagt gggctgaagg cacacattta gaaccagatg aaaaatatgg acataaatat
    14641 ttagaagtga caaaggaagt tttaggagca caatattagt agataccttt aataaatagg
    14701 tttttaaatc aaaatatata gttttactat aattagaagt tgtagattat aatctcttaa
    14761 tacaatttgt atagaaattg tgttaagagg ttatttacat gctaatcacc tattaatgtt
    14821 ttctgagtta attaaaattt tcaaggaggt attttattga ataaactaaa tagtttaatt
    14881 aaaagattat atcacatgtt accattgagt tatgaaaaaa aaattctgtt aaaaaataat
    14941 gtgtataaac tattatctcc atttataaaa aatacaaata tgtatagcat ttggattaat
    15001 agtttagaaa atccagaaaa atatataaaa gataataacc caaagaaggg tgtagataat
    15061 gaaaaagata tttatataga taatttattt aaacgagctt cagagaaatc tagtaactat
    15121 attgaaataa gtgatacaag gtttcaatta gaaaaaaatg atataaagct gatatctttt
    15181 tatttaccac agtttcatcc aattgtagaa aatgatgaat ggtggggaag aggatttaca
    15241 gaatggacaa atgtgagtaa agcagttcct caatttttaa atcattatca accacatcta
    15301 ccagcagaat tgggttttta tgacctgaga ttaaaagaag ttatgaagag acaaatagag
    15361 ttagcaaaaa tgtatgggat atatggtttt tgttttcatc attattggtt tgcaggtaaa
    15421 agacttttag agagaccagt aaatatgtta ttaaaagata aagaattaga tattcccttt
    15481 tgtctatgtt gggcaaatga aaattggaca aggcgttggg atggtttgga aaatgaagtt
    15541 ttaatagaac aaaagcactc aaaagaagac gatttagaat ttattaaaga tataagtaat
    15601 tattttaaag atgagagata tataaaaatt gatggaaagc cagttttgat tatttataga
    15661 atagaattat taccaaatcc aatagaaaca ataaaattgt ggagaaaata ttgcctagaa
    15721 attggctgga atgatatatt cattgtggga gcacaaacat ttggatttga agaacacatt
    15781 aaatatggaa tggatgcagg tgttgaattt cctcctcata atataaataa ttgtcctata
    15841 ttaaataagg aaataaattt tacaaataaa aaatttactg gtttagttta cgattataaa
    15901 aaattagttg atgataaatt atatataaaa aaatcacatg gaaaggtatt taaaactgtg
    15961 atgccttctt gggataatac agctagaaag ccaaataatg caagtatatt taaaaattct
    16021 acaccagagt tatataaaaa atggttaaaa gatattatat attctacaaa agaaaatgtt
    16081 ttagatagtg ataactttat atttataaat gcttggaatg aatggggaga aggtgcacat
    16141 cttgaaccag atagaaaata tggctactca tacttaaatg caacaagaga agctataata
    16201 gagactagga gtgagaaaaa taattaaaaa ttagttattt aaatagatgt ttaaggagag
    16261 ttaaaatatg gataagttaa aaaaaaataa taacctaaaa attaaaataa tattttttat
    16321 attattacaa atatttttta tatttttttt agttgtaaat ttcaaaactc ctcttttggg
    16381 agaagattat gctttactat ataatggtcc atttaaagat ataggtgact atttttcgac
    16441 aattatggca gctcttgaaa agcagatgtc aacttggatg gttagaatgg gaaatccatt
    16501 aacaataata tggctaagct ttgataaaag catatataac atagcagttt catttgtatc
    16561 aatgattttc ttttttctta tattttttta tggaaatgca agaaaaccaa atgtgaataa
    16621 tctgggtgat ttaataggtt tttttgttcc agcatcactt atgatgtttt tgagtccttc
    16681 tataggggat ttgttttttt ggtgcaatgg cagtcttaat tatttatggc ctctttgtat
    16741 attacttttg tgtgcaattc cttatagact ttatttatca gagaagaaca taaatattaa
    16801 gaatcctatt ttgattattt tatattgtat agcttgcttt atatctggtt ttacaaatga
    16861 aaatactcca ccagttatta taacattagt agctgcattg ataataagag atttgtattt
    16921 aaaaaagaaa ccacctaaat ggatagtttt ttcctttata tctatttgtg cagggtggat
    16981 aggattagta gtgaattcaa ccaccagatt tagaagtatg tattatagga atatgtttgg
    17041 cttaccagaa catgctacta taaatgaact tataaataat gttttaagag taggatattt
    17101 attcttgaaa agtggatttg catttgtaat tatattttta attttaagtt tgttatttat
    17161 atttatatta aagtctcaaa aaactgaaaa tgagtttaaa tataagcatt tgtttgcaga
    17221 aaatatgttt tttattttat taacttttat ctctgcagca gcattagttt ttgcaccata
    17281 tacagaactt agatcattta cacttattca tacaatcata ttagcaacaa taagtaattt
    17341 attagtaaat ttatatgaaa tatctagtca taaacaaaga cttggactga gtagtattag
    17401 tataattact ttactttcta cttttttact ttttattaac atatacaatg aatataatga
    17461 ctattataaa tttgatactc aacgtacttg tgatataaag tcacaagtag cagctgggaa
    17521 aactacagta tatgcttcaa aaaattttat tgaaaatcgt actatgaata atagagaatt
    17581 ttatatctac ggaaatgatt taggacaagg tggaaatttt tactcagatt actttggagc
    17641 taaaaaaata atctggtatg ataaaattga tagtaatata gatccagcta ctattaattt
    17701 atcaagtgaa tctacagatg tgaatgtaga acttataaat ggaaactctg aacaagacaa
    17761 tagttctcta ttatctgatg ggatttttaa tgtttctggt tgggcaatag ataaggtggc
    17821 tggaaactct ccaagtaaag tatttataaa aatagacgat aagtattatc aagctagaac
    17881 agtaatacgt gaagatgttg ctaaatattt tgacaataaa aagtataaaa aagcagggtt
    17941 tgagtgttct ataccaacta aagatataaa aaaagggaag cataaagtga cactttgtgt
    18001 cataagtaat gatggagaaa aaagatatga agtagattca ggaaaatcta tagaatttaa
    18061 ataaaagcta taaaatggaa aggaggacat gtaaatggca agtttaatta aacaaagaaa
    18121 atataataga aatatattat tagaatatat taaattaatg agaccaaaac aatatatcaa
    18181 aaacggattt gttttagctg cattaatatt ttcaaacaat atactcaatc catcattagc
    18241 gttaaaatct atattatctt ttatagcatt ttgtatgata tcatcatcag cctatatact
    18301 aaatgatatt ttagatatag aaaaagataa gatgcatcct aaaaagtgca agagacctct
    18361 agctagtgga tcaattggaa agaagggggc aatttcatta ggtattgtat tagttttatc
    18421 ttcaatttta ttatccttat taatacataa aaatctatgt gtaataatat tattgtactt
    18481 atttaacaat attatgtatt cactcaaatt aaaaaatata atattaatag acgtattttc
    18541 tatagccata ggattcatac tgagagtttg tgctgggtct atagcaataa atgttagttt
    18601 atcaagttgg ataattttat gcacattctt tttatcttta tatttaggtt ttgggaagcg
    18661 aaaaaaagaa ataatattac ttaaagaaga tgcaagtaat catagaaaaa tattaaaaga
    18721 atatgatgaa gaaaacctaa atcaaatgat gtcaatatct ttaagtgcga ctatagtgtg
    18781 ctactcatta tactcagcaa ataacattta taattcaaat atgatattta ctactgtgtt
    18841 tgttgtatat ggtgttttaa gatataatta tatagttaat ataggggatg aaggtaatcc
    18901 tactgatgtt gtattaaatg ataaaagttt aaagttttgt gttttatttt gggtaattgc
    18961 gtgtattgga attttaagta cttagagtta attacatatt aaaataaaat ttaaagttga
    19021 aaagatggag aattttcatg ataaatatag taataccaaa ttataatgga tataaacttt
    19081 taaaaaattg tctagattca ttaaaaaatc aaacatattc taattttagt ataatagtag
    19141 tagataatgc atcagatgat agttcatata attggctaaa caactatgaa aatgttacac
    19201 tgaaaaaatt agataagaac tatggtttta gtaaggcagt aaatgaaggt ataaaattat
    19261 ctaattcgaa atatgttgtt ttgttaaaca atgatacagt agtacctgaa aattggttag
    19321 agacactatt aggagagatt gagaaagacg agagtatttt ttccgtatgt agcaaaatga
    19381 ttagatataa tgaaaaggac aaaatagatg atgctggaga tgaatattct atactaggct
    19441 gggcttacaa aattggagat ggtaagaaca tatcaagttt tagaaaaagt aaatatgtat
    19501 ttagttcttg tgcaggtgct gctatatata gaaattcaat ttttgatgag ataggttatt
    19561 ttgatgaaga tttttttgca tatatggaag acattgatat atcttataga gcaaaaattt
    19621 atggatataa aaatagatat tgctcagaag caaaaatatt tcatataggg agtgctacat
    19681 ctggaagtaa atataattca tttaaagtaa gattggctgc tagaaacaat gtatatgtac
    19741 catataaaaa catgcctttt ttacagctta taataaacat gcctttttta attattggtt
    19801 ttttagtgaa gtacttgttt ttcttgaaaa aaggatttgg aaacgattat aaagagggtt
    19861 tttttgaagg tatcaagaat ttaaagaatc taaataagac agaatttaga ataaaaaatt
    19921 tatttaatta tttaattata gaagctttat tgatattgaa tatgtgtaaa tatatatttt
    19981 caaaagtact agatatttat aattaaatat atcatttgat tattcattaa gaagtaatgc
    20041 atacttaaga taatatggat ctattataaa tatacatttt ttgtattagt gatatttgaa
    20101 ttttttacta gaaataattt gtatttttat gtcaatctta atttaaatac aaaaaaatat
    20161 caaagtat
//
LOCUS       DAGSWI010000068        20022 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.068, whole genome shotgun sequence.
ACCESSION   DAGSWI010000068 DAGSWI010000000
VERSION     DAGSWI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.068"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..411
                     /locus_tag="MJF80_002268"
     CDS             <1..411
                     /locus_tag="MJF80_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBZ0280926.1"
                     /translation="VAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNR
                     KLSRLISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVKDLNIR
                     EWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            529..834
                     /locus_tag="MJF80_002269"
                     /pseudo
     CDS             529..834
                     /locus_tag="MJF80_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            847..1752
                     /gene="truB"
                     /locus_tag="MJF80_002270"
     CDS             847..1752
                     /gene="truB"
                     /locus_tag="MJF80_002270"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBZ0280927.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            1883..2812
                     /locus_tag="MJF80_002271"
     CDS             1883..2812
                     /locus_tag="MJF80_002271"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBZ0280928.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            2916..3173
                     /gene="rpsO"
                     /locus_tag="MJF80_002272"
     CDS             2916..3173
                     /gene="rpsO"
                     /locus_tag="MJF80_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBZ0280929.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            3413..4258
                     /locus_tag="MJF80_002273"
     CDS             3413..4258
                     /locus_tag="MJF80_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBZ0280930.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            4383..6485
                     /gene="pnp"
                     /locus_tag="MJF80_002274"
     CDS             4383..6485
                     /gene="pnp"
                     /locus_tag="MJF80_002274"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBZ0280931.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETGMIP
                     RAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEARPS
                     RGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTLSLL
                     DAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQMDI
                     KIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDKIKD
                     VIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVGKTY
                     KGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEIDEKG
                     RVNLSRKVLLPKPEHKNK"
     gene            6751..7494
                     /locus_tag="MJF80_002275"
     CDS             6751..7494
                     /locus_tag="MJF80_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBZ0280932.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            7652..8899
                     /locus_tag="MJF80_002276"
     CDS             7652..8899
                     /locus_tag="MJF80_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBZ0280933.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINLIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            8934..9179
                     /locus_tag="MJF80_002277"
     CDS             8934..9179
                     /locus_tag="MJF80_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBZ0280934.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            9189..10412
                     /gene="dapG"
                     /locus_tag="MJF80_002278"
     CDS             9189..10412
                     /gene="dapG"
                     /locus_tag="MJF80_002278"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBZ0280935.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10525..11259
                     /locus_tag="MJF80_002279"
     CDS             10525..11259
                     /locus_tag="MJF80_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBZ0280936.1"
                     /translation="MKDYREDNEKFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            11382..13793
                     /locus_tag="MJF80_002280"
     CDS             11382..13793
                     /locus_tag="MJF80_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBZ0280937.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDDEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            13796..14854
                     /gene="mnmH"
                     /locus_tag="MJF80_002281"
     CDS             13796..14854
                     /gene="mnmH"
                     /locus_tag="MJF80_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBZ0280938.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            14848..16182
                     /gene="rimO"
                     /locus_tag="MJF80_002282"
     CDS             14848..16182
                     /gene="rimO"
                     /locus_tag="MJF80_002282"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBZ0280939.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16169..16711
                     /gene="pgsA"
                     /locus_tag="MJF80_002283"
     CDS             16169..16711
                     /gene="pgsA"
                     /locus_tag="MJF80_002283"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBZ0280940.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            16999..18045
                     /gene="recA"
                     /locus_tag="MJF80_002284"
     CDS             16999..18045
                     /gene="recA"
                     /locus_tag="MJF80_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBZ0280941.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            18221..19762
                     /gene="rny"
                     /locus_tag="MJF80_002285"
     CDS             18221..19762
                     /gene="rny"
                     /locus_tag="MJF80_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBZ0280942.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8187 a         2031 c         3875 g         5929 t
ORIGIN      
        1 gttgcaggac ttcaagaaca tatagctaat caaagaaatg attttctaca aaaactatct
       61 actaaattaa taaaagagaa tgatattatt tgcatagaag atttacaagt aaagaatatg
      121 attagaaatc gtaaactatc tcgcttaatt tcagatgtat catggtcaga atttattcgt
      181 caattagaat acaaggctaa ttggtatggt agaaaaattg taaaggtagg taaattcttt
      241 gcaagttcac aaatatgcaa taaatgtggg tataaaaacg aggaagttaa ggatttaaat
      301 ataagagaat ggatttgtcc tagttgtaat gaaactcacg atagagatat aaatgcaagt
      361 ataaatatat taaaagaagg attaagacta ataacgattc aaaataaata acttatgtaa
      421 gaaccgtagg aactacgggg atagcctgtg gagaattagt aagacatatt ttaatatgca
      481 aaattctgtg aagcaggaac cctgtgactt tagtcatggg aggttcagtc aaaggtatct
      541 agtatagttt taactcaaga catgatgaat agaaataata ttagctttaa tgatgttgat
      601 ggaataacaa catatacaag agacataaaa ggtatagaag ttggaatttt atttaaagag
      661 aaaaaacaaa atgaaattaa agtgagtttt aggtcaaaaa attatgttga tgtaagtgaa
      721 atagccaagt tatttggcgg tggtggtcat gtaagggctg ctggatgtac tattagagat
      781 agtattgata atgcaaagaa aatggtacta gaggcagtgt taaaatcaat ttaaggtgac
      841 agtaaaatga ataagattat aagtatattg aaacccacag gtatgacatc tcatgatgtt
      901 gtaagtagag tgagaaagat tttaaatata aaaaaagttg gtcatacagg tactcttgac
      961 ccagatgctt caggtgtttt accaatctgt atagggaaag ctacaaaagt atgtgaagtt
     1021 attttaaaca aagataaatc gtatatatgt gagcttactt taggtataag tacagatact
     1081 tatgatgctt caggtgaaat tttaaaaaaa gttgatgatt ttaaatttag taatgaagac
     1141 atagaaagag cttttgacac acaaagagga gagataaatc aacttcctcc aatttattca
     1201 gctttaaagg taaatggaaa gagaatgtgt gatttagtaa gaagtggtag acaatctgaa
     1261 attactttaa aaacaagaag agtaaatata aaagacataa aaatactaag tataaaaggt
     1321 aataaagtaa tgttttatgt tgagtgttct aaagggactt atgtgagaag tatttgccat
     1381 gatataggag aatatctagg ttgtggagct catatgtcgt ttttgaatag aacatcttct
     1441 ggtaaatttg acttagataa ttcaataaca ttagaagaat tagaattatt ttatgaaaat
     1501 aaaactttag acaaatactt atatgatata gattatgtcc ttgattcgtt taattatgtt
     1561 gtattaaacc ctaatgctat aaaatattat agtaatggtg gctcaataga tgataaaagg
     1621 tttttaaaaa ataatttcga taaagatgat gaatttgtaa gagtatacag tacagataat
     1681 ttcttaggat taggaaagtt atcaaaacac aataatacaa taagtgttaa aagtgataag
     1741 atgttcatat aaccaatgta tttaaaaaaa caacaaaaga gatagtacta taataacttc
     1801 tatagtctat ctcttttact tatgctataa tagtttgtga ataagttaag agtaaattat
     1861 attttagcgg ggaacattta ttatggtgat tataaaatct ataaaagaaa tagcaaacat
     1921 aaaagaaagt gtcattacca taggaaattt tgatggtgta cacaaaggac atcaagttct
     1981 tataggaaaa acagttgaac atgcaaaaaa agaaaatata cagagtattg tatttacatt
     2041 tgcaaatcat cctgtaaatt actttagacc aaactcaaca aaaaatatta taagcaatga
     2101 tgataaaata aaattattag aagatttggg aatagatata gttgtaatca taccatttga
     2161 tgagtatatg actaaaatac ctgcaaagga tttcgtagat gagatactag taaaaaaatt
     2221 aaaagctaaa aagatagtaa ttggacatga ctttacattt gcaagggcaa aagaaggcaa
     2281 tgttaatcta ttaaaaagct tagaacatga atttggtttt gaggtagaag taataaaacc
     2341 catcaaaatc aatgatgtaa gagtgagtag tacttatatt agaagtttag tatcccaggg
     2401 tgatatggct aatgtcaagg aatatttggg tagaaattac aagttagaag gttgtgttat
     2461 acattctaaa caccttggaa gaactatagg attcccaacg gcaaatattg accttaagaa
     2521 taatatgctc gttccaaaga gaggtattta tgcttctatt gtacatattg gagatgagac
     2581 ttattttggt gctacaaatg taggctataa cccaacagtt aatggaaaaa ctttgtctat
     2641 tgaaacaaat attttagagt ttgatagaga tatttatggg gaaaatataa ttgttgaatt
     2701 tttggaaaga ataagagatg aaagaaagtt taattcaata gatgacttaa aaaatcaatt
     2761 gtataatgat acaaattatg tgtatgaaaa ctatgtttgc aaaaacaagt aatgtatgat
     2821 aaaatgatta aggaaaataa ccattactcg gcatttgaat actccaatca atgcttagta
     2881 ataggcgttt ctaaaaaaat acggaggtaa tctaaatgat aaacaaatca gaaataataa
     2941 aagaatatgg aagagctgaa ggggatactg gttctccaga agtacaaata gctttattaa
     3001 cagctagaat aaacgagtta aatggacatt taaaaactca taagaaagac caccattcaa
     3061 gaagaggact attaaaaatg gttggtagaa gaagaaactt attagcttac ttaaaggaaa
     3121 aagatcttga aggatataga gcattaatag ctaaattagg attaagaaag taatttcatt
     3181 ctttataaaa aagcaggtat agtgtatacc tgctttttta tagttaattc attataaagt
     3241 aaaatagtta ataataaaag tagtttatat gttataaaaa attataaaat gaataatatt
     3301 gggtaagaat attaaaatag tatacaaata cataatcaat ttaaaaggaa agacaggata
     3361 aatatagaaa ttttataaca ggataattag ttggaataag gaggatatta ttttgaaaat
     3421 aaagttagta tgcgatagtt tgtgcgatat tccagatgaa atatcggaaa aagattatat
     3481 agagatagtt ccacttacag ttatttttaa tgatagggaa tatatagaag gagtagatat
     3541 aaagaaagaa gagttttata aaaaagtaaa agaaatcaag caaataccta aaacttcaca
     3601 agctacatat atggagttta aagaagtgtt tgataagttc ataacagaag gttatcatat
     3661 aatctgtatg acaggggctg caaacgcatc aggaactttt caaagtgcta tgatagctaa
     3721 aaatgatgta aatgaaaatg aaaaaataca tatatttgat actagaaatt tatctctagg
     3781 tagtggacaa tatgttataa aggcatgtga acttcttgaa gaaggactgg gatttgaaga
     3841 aattatagat gaattagaaa atactcgcag tagtgtaaga ttgctttttg caccatatac
     3901 acttgatttt ttaaagcaga gtggaagagt tccagttgca actgctttga taggaaatat
     3961 gttaaatata aagcctatat ttttctttga taatggggaa gctaagttgg taaataaggt
     4021 tagaggaata aagaatatag caagtaaatt ggtcgatata attttagaaa tgaatgaagg
     4081 gtctttagaa ggaaaaatcg tcacaatagg ttgtggagat aatttacacg attgtgaaat
     4141 attaaaagat gaagtaaata aaagaataaa agcaagaaga gtattattta ctcgaggagg
     4201 agtttctatt tgctctcata cagggccaga tatattggct ataagttgtt ctaattagta
     4261 gtaatataag gtttttgcat atatatgtga agataatttt atattgttaa tttatagtat
     4321 ttttgataat atagtaaata gagtgatata cgtgtttttt aataagagga ggacaagcat
     4381 taatgtttga acataaaata tttaaaatgg attttgcagg aagagaactt tctgtagaaa
     4441 taggaaagat ttgtgaaatg gctagtggta gttgtatagt tcgatacagt gatagtatgg
     4501 taatggtaaa tacaactaaa tcagccaaac ctagagacgg aatagacttt tttcctttaa
     4561 gtgtggatta tgaagaaaaa ttatactctg taggtaagat acctggaggt tttttaaaga
     4621 gagaaggcaa gccttcagag aaagctatac ttacatcaag attgatagat agaccaataa
     4681 gacctttatt cccaaaagga tttagaaatg atgttcaggt agttgcaaca gttctatcag
     4741 tagaccaaga ttgtacacca gatatagtag caatgatagg ttcttcaata gccctttcta
     4801 tatcagacat accatttaat ggaccaacag gttcagtatg tgttggcctt gtagatggtg
     4861 catttgttgt aaatccaaac gcagagcaaa gagaaaaaag ttcaatgcat ctagttgttt
     4921 caggtacaaa ggaagcaata atgatggttg aagccggagc tgatgaagtt ccagacgagg
     4981 taatgcttga tgctatttta tttgctcacc aagaaataaa aaaaatagtt gagtttatag
     5041 aaggtatagt tgcagaagtt ggaaaagaaa aaatgcctgt tgaattatac catgcaggag
     5101 aagaaataac tcaattggtt cgcgaatttg caactgataa gatgaaaaaa gcagttcaga
     5161 cttttgaaaa gttagagaga atggaaaata tggatagagt taaagaagaa actttagctc
     5221 actttgaaga aacttttgaa gattttgttg gagatataga agaagtatta caagatataa
     5281 taaaagaaga agtaagaaaa cttatagtac atgaaaatgt aagaccagat aatagaaaac
     5341 ttgaagaaat aagacctata tggtgtgaaa ctggcatgat acctagagct catggttcag
     5401 ccatatttac tagaggacaa actcaagtac ttaatgttgc tactttaggt gctttaggag
     5461 atgttcaaaa actagatggg ttagatgaag aagaaaacaa gagatatatg catcattata
     5521 atttccctgc atatagtgtt ggagaagcta gaccatcaag aggaccagga agaagagaaa
     5581 taggacatgg tgctttagca gaaagagctt tattaccagt aataccatct caagaagaat
     5641 ttccttatgc tattagatta gtttctgaag tattaagttc aaatggttct acatctcaag
     5701 caagtgtatg tggctcaaca ttatctttac ttgatgcagg tgtacctata aaagatatgg
     5761 ttgcaggtat agcaatggga cttataaagc atgatggtaa ggttgcagtg ctttcagata
     5821 tacaaggtat ggaagaccat ttaggcgata tggactttaa agtagctgga acagagtatg
     5881 gtataacagc aatacaaatg gatataaaaa ttgatggtat agataaagaa attctacaac
     5941 gtgctttaaa acaagctaaa gagggtagaa tccatatact aggagagatg agaaaaacta
     6001 tatctcagcc aaaaccagaa ctttcaccat atgcacctaa gattgttaaa atgcaaataa
     6061 atcctgacaa gataaaagat gtaataggac ctggtggaaa gataataact aagataatag
     6121 acgaaactgg agttaagata gatattgaac aaactgggga agtatttata tcaggtattg
     6181 agattgatat gattaagaaa gctcaagaac ttataaataa catagtagtt gaaccagaag
     6241 taggaaaaac ttataaaggt aaggtaagta gaataatgaa ctttggagca tttgttgaga
     6301 tacttccagg aaaagaagga ttacttcata tatctcatat agctcatgaa agagttgcca
     6361 aagttgaaga tgtattgaat ataggggatg aagttgaagt taaagttaca gagattgatg
     6421 aaaaaggaag agttaattta tcaagaaaag ttcttttacc aaaaccagaa cataaaaata
     6481 aataacttat tatataatta aaagcaaagt cttaggattt tgctttttta aatacaaaaa
     6541 agtagataat tttaaaatga tagttatgga tggcttatag ttctttttct gtcaggcttt
     6601 actcttataa agaagctatt taaaagctga ttaatagaga gctttattga tatatataga
     6661 atcacttaag ttatatttag tgcataaaca gagcttattt ttaatagtat taaataagaa
     6721 acaatttgta cataattagg gggagtttta atgataatga tggtacttaa taagagtaag
     6781 ctcaaaaaaa tcataatact aattttagta gtgttattag tattatcagg aggggtaatg
     6841 gtattaaagt tatttaacaa caagccagta tttaatttat ttaatggtgt agatttacca
     6901 tatgaaagag gtacaaagga taaaagtgga tatgttgcat taacctgtaa tatagacctt
     6961 ggatgggaaa cagagtacgt agaatccatt ttagaaacac ttaaaaaaga aaatgtaaag
     7021 attacattta atgtaactgg aaaatgggca gaaaagaata aggatgaatt attaaagata
     7081 aaaaaacaag gacatgaaat aggtaatcat ggatataaac atttagatta ttctacctta
     7141 tcatatgaag acaattatga gcaaatagag acctctaaaa agataattga agaaattata
     7201 ggtgaaaaaa ctaaattttt tcaagcacca gcaggttctt ttggtccaga aacagtaaag
     7261 gctgctaaag cattagggta tacttctatc aaatgggatg ccgatactat agattggaaa
     7321 tacaaagacc aaccagaggt aatcattgat agaatgaaga aaaaagacat taaagatagt
     7381 agtataatct taatgcatcc aaccaacgca acgacaaaat gtatagatga tattatagct
     7441 attgtaagag aaaaaggact aaaaccagga aaacttagtg atgtatttaa gtagacaaag
     7501 tataaatatt tggttaagat tgtataagtt gtagttgaat taattaaaag actgacttat
     7561 aatgtaatac atataaaaaa taaatataat taaaacatag tgttttcaat aatccaatat
     7621 gaaacaaaga ataaattgtt aggagaatgt aatgtataag acaaaaatac tggaaaatgg
     7681 gcttacaata attggtgaag aaataccata tctaaagtca ataacattag gtatttggat
     7741 aaatgcaggc tctaggatag aagaagcaca ggtgagtgga acttctcatt ttatagagca
     7801 tatgatgttt aaaggaacca aaaacagaac atcaaaagag attgctagtt caatagataa
     7861 tttgggtggt caaataaatg catttacaag taaagaatgt acatgttatt atgtaaaact
     7921 aatagatgag catatagata caggaattga tgtattgagt gatatgatac taaattctaa
     7981 atttgataaa aatgatatag ataaggaaag acttataata ttggaagaac ttaagatgta
     8041 tgaagactct ccagatgact tatcttatga tttattagtg gaaaatatat atgctaatga
     8101 tggattagga atgaatataa ttgggacaaa agaatctcta tacaatataa ctagagaatc
     8161 catgttagaa tacttaaata agtattatat tccaaataac gctgttattt ctatagctgg
     8221 aaactttaac tttgatgata tggtggagaa aatcaaatca aaatttggac actgggaaaa
     8281 gaaaaattta agtatagaca taagtgaggc taagtttaat ccttgcttta tatcaaaaaa
     8341 taaagatact gaacaggtga acttagctat gtgcctaaaa ggtattcctt ttgaaaatga
     8401 tgaagaagtc tattcaatgg cagtggtaaa taatattttc ggtggaagta taagctcaag
     8461 gctttttcag aaaataaggg aagaaaaagg tttagtatat tcaatatact catcacagac
     8521 attatatagg aaatgtggtg aattaggtat atttgcaagc atgagtacag aaaatttaca
     8581 agatgtatat aatttaataa agaaagaaat tgaaaatata agagaaaact atcttacaga
     8641 aaaagaaata tctgaaagca aggaacagtt aaaaggaaat tatatattgg atttagaaag
     8701 tactagcagt agaatgatgt ctactggtaa atctatgtta cttagtaaaa aagtaaaaac
     8761 tacagatgaa atacttgaat gtataaataa tgttaatatt aatttaataa agaaggttgt
     8821 tgataaggta ttcaatatcg aaaatattgg tacatgtata gttggaagag atgtagaaaa
     8881 gattctacac ttggattaat attgaaaaaa gtaaatctct ggaggtataa aatatgaatt
     8941 tatctgaaat agcaggtaag gaaatagtta acttggtcac aggagaaaga ttgggggtca
     9001 ttggagaatg tgaccttata ctagatgaaa ctacaggcgg tatattagcg cttctaattc
     9061 caaaagaaaa aggatttttt gccagaagaa aagataagtc atttttagaa gtaccatgga
     9121 gaaatgtaaa aaaaataggt aatgacatga taattataga atatgaggga tatatttagg
     9181 aggaaaaaat gagtatttta gtgcaaaagt ttggtgggac atcagtagaa tcatatgaaa
     9241 aaatgaatga agtatgtaag atagtaaaag catacaaaaa aaatgatgaa gaattacaat
     9301 tagtattagt agtttcagca atgggaagaa aaggagctcc atatgctaca gatacactta
     9361 taaatttgtg tagtgcagta aatgatgagc caagtaaaag agaacttgat ttaataatgt
     9421 cttgtggaga aataatatca ggaacaattc ttgctaattt attgaatgca caggggatag
     9481 attctgtttt ctttactggt cagcaagcag gtataattac aagtgatgaa tattctaatg
     9541 caaagataaa gtatataaat cctaaaaagg taaaaagagc tttagatgat ggtaaggttg
     9601 tagttatagc tgggttccag ggtgtaacag atgatggaga aataactact cttggaagag
     9661 gaggaagcga tacatcagca gttgctattg gtaaagcttt agaatgtgaa acagttgaaa
     9721 tttacactga tgtggatggt ataatgactg cagaccctag agtagaacca aacgctaaag
     9781 tattgagttt tatagactat gaggaagtat tccaaatggc tgacaaaggt gcaaaagtaa
     9841 tacatccaag agcagtacag ttggctaaaa gtggaaatat aactttagcc ataaagaata
     9901 caatgaatcc aacttttgaa ggtacaaaaa taggttcttt atgtagacac ttagaagaca
     9961 atatagaata tgaacaagaa agagatttta aggttgctgt tgcaaataaa gatagcgtag
    10021 cacaagttaa gattaagtca gcagaagaag tgtttacaga agtattaaat gagatagaaa
    10081 agaaacttat aactatagat atgataaatt tctttatatc agaaaaagca tttgttgtag
    10141 aagatgctga tataaaatca ttaaaggaaa tactagataa atttgaatta gattatgaag
    10201 taaatagaga ctgtgctaag gtaacattga tatgttcaag aattgctgat gaaatgtctg
    10261 gtatcatgtc aaaggtagtt agaggattat caaaagcagg ggtatccctt ttacaaacat
    10321 cagactcaaa tatgactata tcttgtttag ttagtgaaga agatatgcat acagcagtac
    10381 atgctataca ccaacaattt tatttaaaat aaactgtata atacaattat tttttaatca
    10441 aatttaagaa tttcatatat atcttaatat atttgattta tttggtaaaa ctaaattaaa
    10501 tataattttg ataggagttt ttttatgaaa gattatagag aagataatga aaaatttgat
    10561 aaagataatt gtagtgaaga atctagttgc gaagagagag aatgtataaa gcaatttggt
    10621 accaatgaaa tgccacctca acctcctaaa gatattcaat gtataactat aattggagaa
    10681 attgagggac attttatagg aaatcctcaa aaaaaagcta caaagtacga gcatataatt
    10741 ccaatgctat actcaattga ggaaagcaat gatgttaaag gagtgttagt tgttttaaac
    10801 actgttggag gagatattga agcaggttta gctattgcag aacttttaaa cagtacatct
    10861 aaaaaagtag ttactcttgt tctaggagga agtcatagta taggtgttcc tcttgctaca
    10921 gcaggagatt actcatttat agcaccaaca gcaaccatga ttatacatcc agtgcgaact
    10981 actggtcttg tgattggtat aaatgagact tttgagtatt ttaaaaagat gcaagataga
    11041 ataattcaat ttataataag aacatcaaat atcaaaaaag atgtacttga aaaacttatg
    11101 catgaaaaag atgaacttgt aagtgatgtt ggtagtgtat taataggaaa agaagctgtg
    11161 gattatggtc ttatagatga agttggagga ctaaaagaag cacttaaaaa attaagagag
    11221 cttataaaag aatctgagga agaaaaaaat aatgattaaa atttgtttaa ataataaaat
    11281 ttatgatata atgatgtaat aaagtaggag tgttagtgtt aaggcattag cactttttgc
    11341 tgtgaagggg taaaacttgc taaaaataag gagtgatatt aatggccacg aaaaagaaga
    11401 aaaaaaagaa ggtaaatacc ttaagtttta atgcagaata tcataattta ataacaatat
    11461 ttataggtgt atttttatta tatagtttaa attctaattc tatgggatgg atacctgtgt
    11521 taatgcaaaa tctttttaag ggactatttg gagggctttc aatagcaata ccatttatag
    11581 taattataac aggactttta ggattttttg atggaaatga atatatatat aggctaagaa
    11641 agactaaact atactacata attattttat ttatatttgt attttatgga ttattaaatg
    11701 caggaactct tcctgtagat agcccactaa aaggtaacat gtttgatgat gttatgaaat
    11761 tgggtgtaag tggacaaggt agtggtttga tagcaacaac tattgcgtat tatatgagta
    11821 aaatatttgg tatagcaggt ggatggttaa taagtatctt cgcacttata ctttctgtaa
    11881 tgtttatatt taatatttct ataaaagatt tggtgtcaaa tgcaaaatca aaatctaaag
    11941 catctaaaga cagcaattta acgtttaaag ataaaattgc aaatatgaaa aaatctgcaa
    12001 tagatatgat gactgatgaa gtagatgata ctactataaa taaaaaacca ggtttcttta
    12061 aagggttaat gtcaaaaggt cttaataatg atgatgatga agatgaatat tttgaagaag
    12121 aaaatagtga tggtgtagat gataaaacta taaaaatagt tggatttaat aaggcagaag
    12181 atgagtattt agaaatatta gaaggaactc aaagtatgcc agagttagat gttttaaaag
    12241 aattacaaaa agctaccaat gaaaatccag ttgtagatac taaaccagaa aaaaaagttg
    12301 atatagctaa acctaattta aatatagaaa aaactcaacc tatgagtatt gtagcagagc
    12361 cagttaatga ggattattca aactataaaa agcctagcat tgagctttta aataaagtaa
    12421 ataagaagtc tgatgaaaat ggtaaaaaga aagtgttgaa aaatgcttct cttttagaaa
    12481 agacactatc agattttgga gtggaagcaa aaattaatca agttacagtt ggacctacta
    12541 taacaagata tgaaatacaa ccaagtccag gagttaaggt aagtaaaatt gtaaatttaa
    12601 ctgatgatat agctttaagt ttagcagcta aaagtataag aattgaagcc ccaattcctg
    12661 gtaaaagtgc aattggaata gaagttccaa atgaagaagc tcaaatggtt ggtgtgagag
    12721 aagttctaga aagtgaagaa tttaataatt ttaactctcc tcttgcaatg ggacttggaa
    12781 aagacgtagc aggaaaaatt ataattggag atataggtaa gatgccacac ttactaatag
    12841 ctggttctac aggttcagga aagagtgttt gtgtaaatac attaataagt agtattttat
    12901 ataaggcaaa tcctgatgaa gtaaaattac tattaataga cccaaaagta gttgaacttg
    12961 caaattataa tggaatacca catctattga taccagtagt tactgaccct aaaaaagctg
    13021 caaatgcttt aaattgggca gttacagaga tgaatagaag gtacaaatta tttgcagatg
    13081 ctcaagtaaa agatgtaaca agttataatg agaaagcgga agaaaaactt cctaaaatag
    13141 taataataat agatgagtta gcagatttaa tgatggcaag tgcaaatgat gtagaagatt
    13201 atatttgtag attagctcaa atggcaagag ctgctggaat gcacttgatt gtagcaactc
    13261 aaagaccatc tgttgacgtt ataacagggg taataaaggc taatatacca tctagaatag
    13321 catttgcagt atcatctcaa actgattcaa gaacaatcct tgatatgggt ggtgcagaga
    13381 agcttctagg taaaggtgat atgttattct atccacttgg tgcagcaaaa ccagttagac
    13441 ttcaaggggc attcatatca gagagtgagt cggaaaaagt aatcgatttt gtaaaaagtc
    13501 aggttaaaga tggaataaaa tatgaagaag atataattga aactatatct aaggttaata
    13561 cttcaaaggg tagtgatgag gatgagtttt tgagtgaagc gatagaattt gtcgtggaaa
    13621 gtggtcaagc atctgcctct atgctacaga gaagatttaa aataggattc aatagagcag
    13681 ctagacttat agattctatg gaagaaagag gaataattgg agcaagtgag ggaagtaaac
    13741 cgagaaaggt attaataagc aaacaggatt tgcagaattt agaaggtgaa tagaattgtc
    13801 agttataaaa atagaaaaag cacttaagct agataaagta atatttgtag atgtgagaac
    13861 tgaaggagaa tatgaagaag accatatatt gaatgcaatc aatatgcctc tttttaaaaa
    13921 taatgaacat aatgaagttg gaacaattta taagatgcag ggaaaacatg aagcaataca
    13981 aaaagggttt gactatgtat cgtataaatt aaaggatata tatttacaag ctgcagaact
    14041 tgcattaaat tatgataata ttgtcatata ttgtgcaaga ggcggaatga gaagtggcag
    14101 tatagtaaat ttattatcat cattaggtgt aaatgtatat caacttgaag gtggatataa
    14161 ggcttataga aattttgttt tagagtattt aaggcatgta atggatacaa aaaactttgt
    14221 atctgttcat ggattgactg gtacaggaaa gactgatttg ttacatttat tagatgaaaa
    14281 aggaatagac gtactggatt tagaaggcat agctaaaaac agtggttcta cctttggttt
    14341 tataactttt gataaaaagc caccatctca aaagaacttt gaaacaaaac tatttgaaag
    14401 tatatttttt tcgaaagaga attatatatt tgtcgagagt gaaagcaaac gagttggaag
    14461 tgttacagtt ccaaatgaaa tttttgaggc aatgacacga gaaggacatc atatattatt
    14521 agaatgtgat attgaaaata gagttgatag actttgtaga gattatattt atgataaaga
    14581 tgaaggtaat atattgattt taaaagaatg tataaacaag tttagaaaaa gattgggtaa
    14641 taaagtagta gatgattata ttgatttatt agaatcaggt gaatataaag aacttgtaaa
    14701 aagatatctt ttagaatact acgacccact ttatatgcat tcagtagaga aatataagta
    14761 tagtaagatt ataaatttta atgatacaca aaaagcttta gaagatgtta tagacttata
    14821 tgagagtata ttggaaggag aaagtgaatg ttaaaaatag cattggaatc attaggatgc
    14881 tcaaaaaatt tagtagatgc agaaataatg atgggaattt taaataacaa aggctataaa
    14941 ttaattggag attttgaaga agctgatgta attatagtaa atacatgtgg gtttatagag
    15001 tcagctaaac aagaatctat agatacgata ataaactttg cagagttaaa aaaaactggc
    15061 aatttaaagt tgttgatagt aacagggtgt ctagcccaaa gatattctga agaattaaaa
    15121 actgaaattc ctgaaataga tgctatagta ggaacaggaa gctatcaaaa tatagataaa
    15181 atacttaaag aattaagtga aatgcatcaa attgtaagcc ttaatgatat agagtttgta
    15241 tttaatgaag atttaccaag atatatatct acacctagct atatggccta tttaaaaatt
    15301 ggagaaggat gttccaataa ttgtacttat tgtataatac caaaactaag aggtaaatat
    15361 agaagtagaa aatttgaaga tataattaaa gaagctaaga aattagctga atctggagta
    15421 aaagaacttg tggttatagc acaggatacg acaaagtatg gttttgattt atatggaaaa
    15481 gaaagattat cagaactttt agaagaactt gcaaaaatag atggatttaa gtggataagg
    15541 gttatgtatt catatccaga atctataaca gaagaattga tacaagttat aaagaaatat
    15601 gataatatat gtagttactt tgatatgcca atacagcatg caagtaacaa tattttaaaa
    15661 ttaatgaata gaaaaactac taaagaagat atattaaata aaatcaatct gataagaagt
    15721 aatataccag atgctatact tagaactaca ataatagtag ggtttcctgg tgaaacagaa
    15781 gatgacttca aacaacttgt ggactttgtt gaagaggtta aatttgatag acttggtgca
    15841 tttgcatatt caagagaaga agatacacca gcagatagat tgccaaacca tatagatgaa
    15901 gaagtaaaaa tacaaagaag agatacttta atgatgattc aacaaaaaat atctgaagaa
    15961 cttaatgaca aaaagatagg aaaaacttat gaggtgttaa tagaagaaca gatagaagat
    16021 aatgtatata caggaagaac acaaggtgat gcagaagaaa ttgacagtat agtgtatgta
    16081 aaaagtgtag acaatcttga agtaggagaa tttgtaagtg ttcaaataaa tgatgcaatg
    16141 gaatacgatt taatgggaga tgttttatat gaacttgcct aacaagttaa ctttgtttag
    16201 aatattttta ataccagtat ttgttttaat tatgttgctc aatataccaa ataaattttt
    16261 aatagcttgt attattttta taatagcttc aattactgat gctttagatg gaaaaatagc
    16321 tagaaaatac aatctagtca ctgactttgg aaaatttatg gacccattag cagataagtt
    16381 attggttatt tcagcactta catgtatgat agaagaccat cttgtaagta gttggatggt
    16441 aattataata gtggctagag agcttacagt tagcatatta agagctatag ctgctgctga
    16501 tggaaaagta attgctgctg gaaacagtgg aaaacttaaa acgataactc aaatggtatc
    16561 tatagtgttt ttattgttag gtgctcagtt tgaaaatgta ttgatattaa atattggtga
    16621 aattctcatt cttatagcta cactacttac attatattct ggatgggaat atctatataa
    16681 aaataaagag ctttttatgt cttctaagta aaaaattatg ctttaataaa taaaaactaa
    16741 tgattaataa ataaaaaata acaacttaaa attttaaaaa tgtaaaaaag ggtatactat
    16801 tatagtatac ctcttttcat tttaagaata ttttcttttg tgtatataat tattaataat
    16861 ggtttataaa taaaatgtaa tgaataaaaa tataatcatt agtagcgttt ttaaattgac
    16921 taggtttgat ttatgttata taattaatat ataacaagag aacaaatgtt tgtagataaa
    16981 tgaagggatg gtatagagat gagtgtagat caagaaaaat taaaagcgtt gaatgaagct
    17041 ttaggtaaaa ttgaaaaaga ttttggtaaa ggttcagtaa tgaaattggg agaagcaaca
    17101 tctatgtcta tagatgttat atcaacagga gcgattggtt tagacatagc tattggtata
    17161 ggaggtctac ctagagggag aatagttgaa gtatatggtc cagaatcttc tggtaagact
    17221 actgttgcgc ttagttgtgt agcatcagct caaaaagatg gaggaatagc tgcatttata
    17281 gatgcagaac atgcacttga cccagtatat gcaaaagctt tgggtgtgga tgttgataac
    17341 ctaataatat ctcaaccaga tacaggtgaa caggctttag agatagcaga ggcattgata
    17401 agaagtggag cgatagatat aatagtaata gactcagtag cagcattagt tccaaaggct
    17461 gaaatagatg gagatatggg tgattctcat gtaggtttac aagctagact tatgtcacaa
    17521 gcacttagaa agttaactgg ttcaattaaa aaatcaaatt gtgttgctat atttataaac
    17581 cagttaagag agaaagtagg aataatgttt ggaaacccag aaactactac tggaggacgt
    17641 gcactaaaat tctattcatc agttagattg gatgttagaa aaatagatac aataaaacaa
    17701 ggtgataaag ttataggtag tagaactaga gttaaagttg ttaaaaacaa agtagcacca
    17761 ccatttaagc aagctgaatt tgatataatg tatggagaag ggatttcaaa aattggagac
    17821 cttttagata tagctgctga tgtagatata gttaaaaaat caggttcatg gtatagttac
    17881 aatgatacta aacttggaca aggaagagaa aatgttaaaa aattcttgga agataattta
    17941 gatttaacta ctgaaataga tgagaaagtt agagcatttt acaatttaaa tgaagaacat
    18001 gaagaatcag gtacttcagt atcaaaagaa attgtagaag aataagtaaa aatacaaaaa
    18061 aataactagt aattaaagac atgccacgct ccctattagc ttgacacaat gttgaaaaaa
    18121 ttataaaatt aagtgaggat aaaatatcta aatttaattt taattattag gaatattttt
    18181 taatcatagt gatgacattt tgggattatt aacgaaggag gtggatgtca tagatagtgt
    18241 agtaatgatt gtaataggtg cagctgttgg tattatagct ggatattttg ttagaaaaaa
    18301 tatatctgaa gcaaaaattg gacaagctga aaacttggcc aaagaaataa tagataaagc
    18361 acaccacgac tcagaaacag tacaaaaaga aaaattacta gaagctaaag aagagatcca
    18421 taagtggaga acagaagctg aaagagaaaa cagagaaaga agaacagaag tacaaaagta
    18481 tgaaaaaaga gtagtacaaa aagaagaagt cttagataga aaattacaaa atttagaatc
    18541 taaagaagta aacctaagtg aaaaattaaa ggtagtggaa aagaaagaag aagaagttga
    18601 agtaataaaa actcaacagt tagaaaaatt agaaagtata tcaggaatta cttctgataa
    18661 ggccaaagaa atcatcttaa ccaatgcgga gagagatgta agacgtgaaa tgtctataat
    18721 gataaaagag atagaaagtc aagctaaaga agaagctgat aagaaatcaa gagaaattat
    18781 aggatatgca attcaaaaat gtgcagcaga tcacgtagcc gaaactactg taacagtagt
    18841 aagcttgcct aatgatgaaa tgaaaggtag aataataggt agggaaggta gaaatataag
    18901 gactttagaa accttaacag gtatagacct tataatagat gacactccag aagccgtaat
    18961 tttatcagga tttgatccaa taagaagaga aattgcaaga attgctctag aaaaattaat
    19021 agcagatgga agaatacatc cagctagaat tgaagaaatg gttgaaaagg cgagaaaaga
    19081 agttgacaat attataaaag aatatggtga acaagctgca tttgaaacta gtgtacatgg
    19141 gattcatccg gaattaataa gattattagg tagacttaac tatagaacta gttatggaca
    19201 aaatgtactt aaacattcta tagaagttgc tcatatagct ggtataatgg ctgctgaaat
    19261 aggagcagat attagattgg ctaagagggc aggtttatta catgatatag gtaaggctgt
    19321 agaccatgaa atggaaggta cacatgttga aataggtatg gatttactta gacgttataa
    19381 agagtctaaa gaagttatac atgccatgag tactcatcat ggagattacg agcctcaaac
    19441 agtagaggct gtattagtta cagcagcaga tgcaatatca gctgctagac ctggtgcgag
    19501 aagagaaact ttagaagcat atataaagag acttgaaaaa ttagaagaaa tagctaattc
    19561 atatgaaggt gtagaaaaat catttgctat acaagctggt agagaaataa gaataatggt
    19621 aaaaccagaa gctataaatg atgaagaaat acatctattg gctagagata tgactaagaa
    19681 aatagaggat gaacttgaat atcctggaca aataaaagtg agcataataa gagaaacaag
    19741 agctatagaa tatgcgaagt aaaagaaata atatctaaat aataagaaaa tatttttaga
    19801 aatttaaatt tgccacaaga tatgataata tatcttgtgg cttttaaatt gtttaagttt
    19861 aattttatat aatgcaaaaa taatctaagt gctaaaagtg ctacttacaa aagattaata
    19921 aaataaagct ttatatatgt attatttata taattatcca agtttgtttg atataatgaa
    19981 gttgtaaaat caatacttta ctacaaaata aatagtaaat ac
//
LOCUS       DAGSWI010000069        19810 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.069, whole genome shotgun sequence.
ACCESSION   DAGSWI010000069 DAGSWI010000000
VERSION     DAGSWI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.069"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            144..1331
                     /locus_tag="MJF80_002286"
     CDS             144..1331
                     /locus_tag="MJF80_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBZ0280943.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            1347..2930
                     /locus_tag="MJF80_002287"
     CDS             1347..2930
                     /locus_tag="MJF80_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBZ0280944.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            2958..4286
                     /gene="ssnA"
                     /locus_tag="MJF80_002288"
     CDS             2958..4286
                     /gene="ssnA"
                     /locus_tag="MJF80_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBZ0280945.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DLRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            4431..5783
                     /locus_tag="MJF80_002289"
     CDS             4431..5783
                     /locus_tag="MJF80_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBZ0280946.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5975..7084
                     /locus_tag="MJF80_002290"
     CDS             5975..7084
                     /locus_tag="MJF80_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBZ0280947.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            7367..8758
                     /gene="hydA"
                     /locus_tag="MJF80_002291"
     CDS             7367..8758
                     /gene="hydA"
                     /locus_tag="MJF80_002291"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBZ0280948.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            8752..11313
                     /gene="xdh"
                     /locus_tag="MJF80_002292"
     CDS             8752..11313
                     /gene="xdh"
                     /locus_tag="MJF80_002292"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBZ0280949.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            11465..12811
                     /gene="rpoN"
                     /locus_tag="MJF80_002293"
     CDS             11465..12811
                     /gene="rpoN"
                     /locus_tag="MJF80_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBZ0280950.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            13593..14615
                     /locus_tag="MJF80_002294"
     CDS             13593..14615
                     /locus_tag="MJF80_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBZ0280951.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            14675..15682
                     /gene="gap"
                     /locus_tag="MJF80_002295"
     CDS             14675..15682
                     /gene="gap"
                     /locus_tag="MJF80_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBZ0280952.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            15904..17106
                     /locus_tag="MJF80_002296"
     CDS             15904..17106
                     /locus_tag="MJF80_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBZ0280953.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            17132..17875
                     /locus_tag="MJF80_002297"
     CDS             17132..17875
                     /locus_tag="MJF80_002297"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBZ0280954.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            17884..19416
                     /locus_tag="MJF80_002298"
     CDS             17884..19416
                     /locus_tag="MJF80_002298"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBZ0280955.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTILDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         7950 a         2273 c         3969 g         5618 t
ORIGIN      
        1 aaataaagtc taaataaaaa tttaataaat aaaatataaa gaaataaaaa atcaatagat
       61 taagtgaaat gctaataaat taaaaaagtg aaatatcaaa ataaaacgtc aacaaattaa
      121 aaattatatg ggggaatatc aaaatgttaa atgaaacaag aaaaaaacaa ttaactgaag
      181 tatgccaaga cttaataaga aatgctagtt attcaggtca agaagaaaac gtagtaaaag
      241 ctatagaaga aaattttgct ttactaggtt ttgatagttg gtcaagagat agatatggaa
      301 atatagtagg atgtataaaa ggtaacaagc caggtaagaa aatattattt gacgggcata
      361 tagatacagt tccagttcca gatgcaagta aatggagtgt tcctcctttt gaagcgagaa
      421 tagtagatgg gaaaatatat ggtagaggaa cttctgatat gaaggggcaa gtaagtgcta
      481 tgatggctgc tgtttcatac tttgcagaag atactaacaa agactttgaa ggtgaattgt
      541 atgtagcagg tgttgttcat gaagaaatat ttgaaggtgt ttcagctaga gagataagta
      601 aggctgttca acctgattat gttgtaatag gtgaatcttc agaattaaac ctaaagatag
      661 gtcaaagagg tagaggagaa atagtagtag aaacttttgg taaaccagct cactctgcta
      721 atccagaaaa aggtataaat gcagtttata aaatggctaa tgtaatccaa aagatacaac
      781 aattagtacc tccaactcat ccagttttag gtgatggaat attagtttta acagatataa
      841 aatcttctcc atatccagga gcatcagtag tgccagatta ttgcaaagct acttttgata
      901 gaagattatt agttggagaa acaagagaag gcgtattagc tccaatacaa gctttattag
      961 atgaaatgat gaaagaagac ccagaattaa atgcaaaagt ttcttatgca gttgaaaaag
     1021 ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt tggttatatg
     1081 atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct ggaatagatt
     1141 cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt gaggctggaa
     1201 taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata gatgagtata
     1261 tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta aaatcagttt
     1321 ataaaaaata attaggagat ggttttgtga gaactttaat aaaacatggg cttatagtag
     1381 atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag aaaatattaa
     1441 aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa ggtagagtta
     1501 tatgtccagg tttcatagat actcatagcc attcagacct agttatttta gttaatccat
     1561 ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa gatggtatat
     1621 ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta gcaggtttag
     1681 atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat ttaaaaatga
     1741 tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga aacgttagaa
     1801 tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa aaaatgtgtg
     1861 aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga cttatatata
     1921 taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta gtagcaaaat
     1981 atgacggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta acttcaatgg
     2041 aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca cattttaagg
     2101 tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta gaaaaagcag
     2161 aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga agtacaatgc
     2221 ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta atagaaagat
     2281 taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga atagaaggct
     2341 gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc gtaaaaacag
     2401 aaaagaataa agatacaatt ggtaagagct tattagaaat tggaaaaatg agaggaaaag
     2461 acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt gggatggtag
     2521 atttctatgg attagaagaa catataatag gattcatgaa aagagacgag caaaatgtgt
     2581 gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct ttccctaaaa
     2641 tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct gtttataaaa
     2701 tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta aaagaaggat
     2761 attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat gattttatca
     2821 actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat tgtgttgttg
     2881 aggaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa atcttagctt
     2941 taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc caagattctt
     3001 taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt gaagatatag
     3061 gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat gcaggcaaga
     3121 aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca tttgctagag
     3181 gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac aatttatggt
     3241 ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat gcaacgtata
     3301 tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt ccaaatgcaa
     3361 ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt agaacgtgtc
     3421 tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt atcaatgaaa
     3481 acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa ggaatgtttg
     3541 ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct tcagagatgg
     3601 ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac ttacaatata
     3661 atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga gtactaaaag
     3721 aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt cttttaaata
     3781 aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca gttggatgtt
     3841 ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact gatggatata
     3901 ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat cacttaaaag
     3961 acctaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat agaaaaatag
     4021 ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat gcagatgtta
     4081 tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg ggacacacta
     4141 tttttggaat gagtggaaga agcgtagata caactattat aaatggaaag gtacttatga
     4201 aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt agagaattaa
     4261 gtcaaaatct atggaataga atttaaataa ttgatattaa tatacaagta agaaacaaga
     4321 atttagatgt taaaaacaga gcttaaacaa aaataggaat ttaaatgaag tatataaata
     4381 aaaaatatac ggaatagaat attaaattaa atatagaagg gagacccaaa atgtctcgag
     4441 ataaaagccc taaaatagct ccagtagatg aaaggttacc tgtatccaaa tcttgggtat
     4501 tttcactaca gcacgtttta gcaatgtgtg caggagcagt agcagttcct atgatgatag
     4561 gaagtgctgc acgactagat catgctagta ttgttttttt aataaatgct tgcttgttta
     4621 tggcaggtat aggaacactc atacaaagtg ttggtatagg aaatattgta ggtgctaaaa
     4681 taccagtaat agaaggaact agttttgccg ctgtggcagg tatgacagca atagcctcat
     4741 catatagtga ttcacaagtt gcaatacaga cagtatttgg ctctgtaata gtagctggat
     4801 tgttttgtat attacttgct ccagtatttg aaaaattaat aagatttttc cctagtgtag
     4861 ttactggaac agttgttatg gtaataggta tttcactatt tccagttggg ataaaatgga
     4921 taactgacaa caaattagaa gcggttgaac caaactcatt agcattagct ttagcagtat
     4981 tggcaataac tttactttca tttaaattct taaaaggaat ttggaatagc acagctatat
     5041 tggtaggtat agtacttggt acactacttt ccatggcagt tggaatggca gattttagtg
     5101 cagtaaatgg agttggttgg cttaacttaa atgtaccttt aaaatttgga atgcctagat
     5161 ttgaaccatc tgcaatacta tcattgtgtt tgattatgtt ggtacttatg acagaatcag
     5221 ttggtaatat gatagctatt catgaaatgg tagacaaaga agtaacaggt aaaaatatta
     5281 aaagaggtct atttggagat ggaatatcta cagcattatc tggaatattc aattcatttc
     5341 caataactcc attcggtcaa aatacaggaa tagtaggtct tacaggtata aaatcaagat
     5401 ttatagcagt ttacgcaggg gttatattgc tagtacttgg attcttacca aaatttgcag
     5461 caataatagg agcaatacct aagcctgttc ttggaggcgt aggatttgca atgtttggaa
     5521 tggtaatggt tggaggagta aaaactttaa gcaaagtgaa atttgatgga actaaaaatg
     5581 gggttatagt agcagttagt gttggtttgt ctatgatacc actaacaaat cctgatttct
     5641 atcataattt cccaacttgg gtacaaacaa tattccatag tggaataaca acaggtagtt
     5701 tatcagcagt gcttttaaac atattcttca atgaactagg ttctaaaaaa gaaaaaaata
     5761 ataaagttga agaagcaaaa taaaaataaa acaagtaaaa atctatattg acttaaagtc
     5821 attaagtatt aataaaaata attcagagat attaataatt caacaaaact atcttatatt
     5881 ttaaataata taaaaaagta aataaataac aaatacaaat aaaagaatac aatctaaata
     5941 ttaaaaatat ttaatttaag ggagagataa taatatggct aatttaaaag ttaatttata
     6001 tggaatggaa tttttaaatc caattatgac agcggcagga cctggagcaa aagatggtgc
     6061 tctctgtcaa gaggcagcaa aaggtggagc aggagctata tgtacaaaaa ctatatctgt
     6121 attaccagct gatgtaccaa gaccatgtat ggcaaaaact aatagtggtt ttttaaacac
     6181 agaattatgg tcagagcttc caaaagagca atggatagag gaagaataca aaaaggtaaa
     6241 agaaacaggg cttccagtaa tagtaagtat gggatattca gcagagcaaa tagctgaggt
     6301 tgctccttta gtaaagcctt atgcagatgc agttgaatta tctactcatt atgtaggtac
     6361 agacgttgca ccaatagtaa atgctttaaa agctgcaaaa gctgttttag atgtaccagt
     6421 atttatgaaa atgagtcctc atactgacat tcaaactatt gcaaaagctg ttgaagaagc
     6481 aggtgcagat ggacttgtaa tgataaactc atttggacca tgtatggcaa tagatgtaaa
     6541 tacaggatac cctataatgg gaagtaaagc tggatacgga tggttatcag gtgcaccaat
     6601 aagacctcta gcaataagat gtatatatga agcttctcaa gttgtaaata taccaataat
     6661 cggtgttggt ggaatcactt gtggaaaaga cgtggctgaa atgatgatgg ctggagcaag
     6721 tgctgttcaa gtatgtacag aagcaatcct taaagggcct tctatctatg gaaaaattgc
     6781 taaagaatta aatgaatttt tagatgagaa tggatatgct gatgttaatg aaataaaagg
     6841 actaactcat aagaaaatgg cagctagagg gtttagaact cattcagtag ctccagtagt
     6901 ggatgataac aagtgtatca aatgtaagca atgtgtaaca agctgtgtat acgaagcttt
     6961 agaagttaca gataagttga acatagatgc agacaagtgt tttggatgtg gattatgtgt
     7021 aactagatgt ccaaaaggag ctttaagcat ctgctacaat caagtagaag ctatgaatga
     7081 gtaataatta tatagaattt tggtaattaa cttagagtaa taggtattag gataataaaa
     7141 tatatcagat aaacaaacac acattatgca ttaataatac agtctaaagt taataatttt
     7201 attatattga tttaactaaa tattagttta gctgaatatt aatttaatta ggtattggtt
     7261 taatcttaat atagatttac tgcgtataag tgaactaact agattacttt aagttaattt
     7321 tttcatataa aataaattga tacattacaa gtaaggtgga gtctatatgg gaattgtttt
     7381 aaaaggtggg aaagtagtaa caagtgaaaa aacttactgt gcagatgtaa gaatagaaga
     7441 tgaaaaaata gtcgatatag gtcttgatat atcaaaagaa aatgatgaaa tagttgatgt
     7501 tagtgattct tatgttatac caggaggtat agatacacac acacactttg acttagatac
     7561 aggcgttact aagacagcag ataactttga gacaggaaca aaagctgcta tagtaggagg
     7621 aactactact atattggatt ttgctacaca agctaaagga tgttctttga aagacggatt
     7681 agaagaatgg catgaaaaag ctaaggataa atcctattca gattatggct ttcatatggc
     7741 tataacagat tggaatgact cggtttgtaa tgaaatggag gatatggtga aagagggagt
     7801 atcttcattt aaactgtata tggcgtataa aggaagcctt caagttgatg atggtgtaat
     7861 ctttgaagca ttgagaaaag cagaagagat aggagggatt attggtttcc attgtgaaaa
     7921 tggggacata atatgtgaac ttgtggataa agcaaagtca gaaaataatt tatctccaaa
     7981 atatcatcaa ttaactagac cagctataat ggaggcagaa gctacatcaa gacttatgaa
     8041 gatagctgaa gttgcaaact ctaaagtata tgtagttcac ttaagctgta aagaatcctt
     8101 agaggaggtt ttaaaagcta agcaaagagg tgtggatgta atagttgaaa cttgtcctca
     8161 ataccttcta ttaaatgatg aattatatga tttagatgga tttgaaagtg ctaagtatgt
     8221 aatgtcacca cctttaagaa ataaagaaaa taatgaaata ttatggaatg ctattttaga
     8281 taatcaaata gacactatag gaacagacca ttgttctttt aattataagg gacagaagga
     8341 acttgggata aacgatttca gtaagatacc aaatggttct ccaggtgtag aacataggat
     8401 gagtctttta tacacatatg gagtctgtaa aaataaaata tctatcaaca agatggtaga
     8461 agtaacatca actaatgcag caaagttgtt tggaatgtat cctaataaag gaactataga
     8521 aataggcagt gatgcagata tattagtctt aaatccaaac aaaaaatcca aaatagaagc
     8581 aaaaaaacaa ttacaaaatg tagattatac accttatgaa gggtatgaag tggattgtca
     8641 aatagaacat gtttatttaa gaggaaaaga agtagttaga gatggagaat taatttgtaa
     8701 tactccaagt gggagatata taaataggcg tctaacaaag ggggctagag aatggtagaa
     8761 tttatattaa atggagagaa agttgttgca aaagaaggca aaaaactttt agacttcctt
     8821 agagaagata tgaatataac ttcagttaaa aatggatgtt cagaaggtgc ttgtggaaca
     8881 tgtatggtta taatagatgg taaagctact aaagcatgtg tatttaaaac tgataaatta
     8941 gctgggaaaa acataacaac tatagaggga ttatcagaga gagaaagaga tgtttttgca
     9001 tattcttttg ctagtcaagg agctgtacaa tgtggatttt gtattccagg tatggtaatt
     9061 agttcaaaag cacttataga cgttaataat aatcctactg aagatgatat aaagaaagct
     9121 ttaaaaggta atatatgtag atgtacaggg tatgtaaaaa taattaaagc tgtcgattta
     9181 gcggcaaaga tttttagaga aaatatagat gtgccaaaag taaactgtaa gggtctagta
     9241 ggtgaaaatc ttcctagagt tgatgcagtt ggcaaagcac ttggatattc tgaatatgta
     9301 gatgatatga gaattgaggg tatgatttat ggttcagcag ttagagctaa gtatcctaga
     9361 gctttagtta aaaaaataga tatagaagaa gcaaaagctt atccaggagt tgttgcagtt
     9421 ctaacagcta aagatattcc aggaactata aaggtagggc acttaaagaa ggattgggat
     9481 actttaatac cagaaggtga aataactaga tacttaggag atggaatagt tttagtagca
     9541 gcagaaacta gagaagcgtt agaagaggcc aaaaagctag ttaagataga ttatgaggaa
     9601 ttaacaccat taagtactac agcagatgca cttagagaag atgctccaaa gatacatgag
     9661 acagggaata tacttgttaa ggaacattta gtaagaggaa acgctaaaga aaagatagaa
     9721 aattcaaaat atgttgtaac taaaagatat actacacctg ctacagaaca cgcattttta
     9781 gagcctgaat gtgcagtagc tggtccttat gaagatgatg gagtaataat ctattcgaca
     9841 gaccaagggg tgtttgccac tcaacatgaa tgtgcagata tgttagggtt accatatgag
     9901 aaggtaagag ttataaacaa aatggtagga ggagggtttg gtggaaaaga agacatgagt
     9961 gttcaacacc atgcagcctt acttgcatac catacaaaga gatttgtaaa agtacagtta
    10021 acaagaaaag aaagtattat tgttcatcca aaacgacatg ccacagaaat ggaatttacg
    10081 acagcatgtg atgaaaatgg atacttaacg ggtatgaagg caacgataat atctgacaca
    10141 ggtgcatatg cctctcttgg aggaccagtg ctacaaagac tgtgtactca tgcagcagga
    10201 ccatacaatt atcaagatat tgatataaca ggaacagcag tatatactaa taaccctcct
    10261 ggaggagcat ttagaggatt tggagttaca cagagttgtt ttgcaactga atgtaatcta
    10321 aatttacttg ctgaaatggt tggaatatct ccttgggaga taagatataa aaatgcaata
    10381 agacctgggc aagttttacc aaatggtcaa tttgcagatg aaggaacagc aatggcagag
    10441 acactagaag ctgtaaaaga agagtatgaa aagcatccac gtgcaggtat agcttgtgct
    10501 atgaaaaata gtggggttgg agttggtata cctgatgttg gaagatgtag attggtgata
    10561 aaagatggca aagttcacat aagaacaagt gcttcttgta taggtcaagg gcttggtact
    10621 gttatgactc aagtagtttg tgaaactgct aacctacttc ctgaaaatat agttcatgaa
    10681 gttccagata ctcatttaac accagactct ggtaatacta cggcttcaag acagacagta
    10741 tttacgggag aagctactaa ggttgcatca aataagttaa aagaagcttt agactctggc
    10801 aaaactttac ttgatttaga gggagaagag ttttatggag aatatgaaag tattacagat
    10861 aaaatgggtt gtgacaaaga aaatccagta agtcatgttg cttatggata tgcaacacaa
    10921 gttgtaattc tggatgaaaa tggaaaacta gaaaaagtag tagcagcaca tgatgttgga
    10981 agagctataa atccaatcag tttagaaggt caaatagaag gtggagtagt tatgggtctt
    11041 ggatatgctc taaccgaaga ttacccagta gttaactcta ttcctacagc taagtttggt
    11101 acattaggtt tatttaaatc tacagatata ccagaaatac aagctttaat agttgaaaag
    11161 aacaaagatt tattggctta tggggcaaaa ggtgtaggag aaatttgtac agttcctact
    11221 gcaccagcag tacaaaatgc ctattatgta tttgataaag aatttagaac tgagttacca
    11281 ctacaaaata ctccatatag taaaaaaaag taacaaaaaa aagaaatatt aaataaaaaa
    11341 atagatgaaa ttgtaaataa aaaaaaccct tttgcataag gtatatttta aatgatagaa
    11401 tatacatata caggagggtt tttcttttat aaaataagtt taataaaatt gagaggtgag
    11461 cagaatgaat tttaataata gtctagaatt aactcagtct cagaagctaa taatgactac
    11521 acaacttaag caatcattat caatattaaa tatgagtaag ttagagttag aagaagagat
    11581 aaaaagagaa gcagagaata acccattagt tgaagtcgag aaaagtgggg agataaattg
    11641 ggaagagtat ataaaagaca tggataggtc aagacgttta gatagaacag agataagcta
    11701 taatcctgat aatgaagtta atctagaaaa tttagttaaa tattcgtcaa atttatatga
    11761 agatttaaaa tttcagataa gcctgtataa acttacagac aaagaattgg aagtctgtga
    11821 gtatataatt gatagtttag atgaggatgg atatttaaga actgaggaaa aagtaattgt
    11881 tgatacctta aagatagatg aagaactttt tgaaaaatgt ttaattagtg ttcagcagtt
    11941 ggaacctagt ggagtagggg caagaagtct ttcagaatgt ctgatgatac aaatggctag
    12001 tttaggaata tataatgaaa ttttagagga aatcgttaca aaagacttaa atttaattgc
    12061 aaataataaa tacaaagaga taagtaaaaa gtacaatatg tcattacaaa aatgtgttga
    12121 actaataaac ataattaaga cattagaccc caaaccagga agaacttgtt cagtagagaa
    12181 aagtgtatac attcaacctg atgtaacagt agaaaaaatt gatgatgaat ttattgtata
    12241 tttaaatgag aaagattctt atcaaataag aatcaatagt tattataaag acatattaaa
    12301 aaattcacag tctgatgaaa gtgcaaaaga atttataaaa gaaagactaa attcagcaac
    12361 aggacttatg aaaaatatag agagtaggaa aactacagta cttaagattg cagaagagat
    12421 agtaaaagct caagatgaat ttttgagaaa agggacaaaa tatataaaac ctttgaaaat
    12481 gaaagacata gcagaaaaat tagaatttca tgaatctact ataagtagag gtgtaaatgg
    12541 taaatatatg ctaacacctt ttggcgtata tgaatttagg tactttttca gtagcgccat
    12601 tgagactgag aataatgaaa tggcatctag tacaagcata aaaaaaatta taaaggaaac
    12661 aataaaagac gaaaataaaa agaaaccttt aagtgatgac catatatcta agttattaaa
    12721 agaaaaagga atcaatgttg caagaaggac agttgccaaa tatcgtgaag aattaggaat
    12781 cccatcttca agtaagagaa aagaatacta ataagtaaaa gacaaatatc agtatggtaa
    12841 ggggaaaagg ctaattaaga gatgaatgta gattgggcta aaaattttat aaagtaaaat
    12901 aatataatat gaaaaagtat taaaatgcta taaggtatta aaaaactggg gaacattaaa
    12961 acattaagat agcaaagagc ataaaaaact aaagaagatt aaaacattaa agaacattag
    13021 agctttgagg aatattagaa ctttaaagaa tattaaaatt tataaaatat aaaaatacta
    13081 aaaagaattg aaaaactggg gaacattaaa atattgtaaa atgctaaaaa actagaaaac
    13141 ataattaaat aagtgcttat aaagaatgat taaattaaat aagtgtttgt aaagaataat
    13201 tagatattag aaaaagaaga atacctagat aaataaacta ttattaaatt taataatcac
    13261 aaaaggaaaa cacgtagggt ataaataaaa atcttaacta gataaaagta aacttagttg
    13321 tagataaaaa taactagaaa taaaattatt tatagtaata aatattagaa tgaaaatata
    13381 aaaaaatata tataaaaagc cttatataaa aagaaaaaat actgtgattt gaaaatggtg
    13441 gagaaatatt ttataaaata aaaaaataaa aaagtttttg aaaaactatt gaaatctata
    13501 cgcaaatcct ataaaataaa aatatcttgt gggacttaaa taaacactag ggacaaaaaa
    13561 agtcccgaaa ggaaagcaat aaggagtatg ttatgaagaa cttattagaa attcaaaaga
    13621 aattgattcc acaggcaatt gaacttatgg aaagaagata ctctatttta agacaaatat
    13681 ctttaagtga accaataggt aggagaacat tatcaaatgt attagagatt agtgaaagaa
    13741 taatcaggtc agaaactgag tttttaaaag aacaaggact tatagacgta gctgtatctg
    13801 ggatgactat cactaaagaa ggttctgatt tgttagataa acttaaggac atcatgagtg
    13861 acatcatggg attgtctaag ttacaagata gagtcagaga aaagcttgga ataaaaaggg
    13921 taatattagt tcctggaagc tgtgatgaaa atggaagttt actaaaagat gtaactagat
    13981 atggatgcga atatttctta ggcattttaa aagatggaga tatcgtttca ataacaggag
    14041 gaagcactat gttggagttt tcaaacgtta ttaaaactga taagagatat ccaaactcta
    14101 caatagttcc agctagagga agtatgggaa ctgatgtaga gatgcaatct aacaatatag
    14161 ttgcttcaat aagtaagaaa ttacattcta attgcaaact tttacatata ccagatgagt
    14221 tggatgaaag tgcaatgaaa actcttagcc aagtgccaga gattaagaca actttggagt
    14281 atattaaaag aacagacatt ttagttttta gtataggtag agcagatgtc atggcaaaga
    14341 gaagaaaact tccagaagac aaggttgatg agatattatc taaaaatgca gttggagaag
    14401 catttggata ttatttcaac aaagatggag aaatagttta caaactgaac actgttggaa
    14461 ttgatttaga aacagttaaa caagtaaaag aaactattgc aatctttgca gggactgaaa
    14521 aagtagaagc cttattagct atatcaaata tgcataaaaa catggttctt gtaacagatg
    14581 aagaaagtgc ttataagata ttgtcattat tataaaagtc aagacctagc acagagctag
    14641 aaaacataca tttactttat aggaggtagc gaaaatggtt aaagtagcta taaatggatt
    14701 tggaagaata ggaagattag cattaagaaa aatgatggag caacaagata agttcgaagt
    14761 tgttgcaata aacgacttaa cagacgctaa aatgttagct catttattta aatatgatac
    14821 agctcaagga agatttaacg gagaaataga agttaaagaa ggtgcttttg tagtaaacgg
    14881 aaaagaaata aaagtaacag cagaaagaaa tcctgctgac ctaccatggg ctgaattagg
    14941 agtagatata gtattagaat gtacaggatt ctttacatct aaagataaag ctgaagctca
    15001 tatacaagca ggggctaaga aagttgttat atctgcacca gctactggag atttaaaaac
    15061 aatagtattc aacacaaaca gtgacatatt agatggttct gaaacagtta tatcaggagc
    15121 ttcttgtact actaactgtt tagctccaat ggctaaagta ttaaatgata aatacggtat
    15181 agaaaaaggc ttaatgacta ctatacatgc atacactaat gaccaaaata ctttagatgg
    15241 tcctcatcca aaaggtgatt taagaagagc tagagctgct gctggaaaca tagttccaaa
    15301 cactacagga gctgcaaaag caataggctt agtaatacct tcattaaaag gaaaattaga
    15361 tggggcagca caaagagtac cagttgtaac tggttcaata actgaattag tttgtacatt
    15421 aggaaaaaat gttacagttg aagaaataaa tgcagctatg aaagaagctt caaacgaatc
    15481 tttcggatat actgaagaaa tgttagtatc ttctgacata atcggaataa gctacggttc
    15541 attatttgac gctactcaaa caaaagttat ggaagtagat ggaaaacaat tagttaaagt
    15601 tgtttcttgg tatgataatg aaatgtctta tacttctcaa ttaataagaa ctttaggata
    15661 ctttgcacaa ttagcaaaat agtcagtttt gagaaaatag aaaagtagat tgacaaagtc
    15721 cgagtttgta gtttaatact acggactcag gacttttttt aagaaaaaat atataaaata
    15781 cacctaaata atgttacata ctatatataa aaatatgata atatgatata tttaataggg
    15841 taaaagtatg tactatatta tacttggcaa taaaaaatta ctttattaaa aggagagata
    15901 tttatgtcaa tgcttaacaa aaaaactata gaagatattg atgtgtgcgg aaaaaaagta
    15961 ttagtaagat gtgactttaa tgtgccatta caagatggtg taattactga tgaaaataga
    16021 ttgaacggag ctttaccaac tattcaatat ttaatttcta aaggggcaaa agttatatta
    16081 tgctctcact taggaaagcc aaaaggagaa gctaaaccag aattatcttt agcaccagta
    16141 gcaaagagat tatctgagat gttaggaaaa gaagttgtat ttgcagcaga cgataatgtt
    16201 gtaggagaaa atgcaaagaa agcaactgaa aaaatggaaa acggagatgt agtattatta
    16261 gaaaatacta gatatagaaa agaagaaact aaaaacgaag agaatttctc taaagaacta
    16321 gcatcacttg ctgaaatatt tgtaaatgat gcatttggaa ctgctcatag agcacattgc
    16381 tcaacagttg gtgcaggcga gtttttacaa gagagagttt gtggatactt aattcaaaaa
    16441 gaattaaaat tcttaggaga agctgttgca aaccctgtaa gaccatttac agctatactt
    16501 ggaggagcta aagtttctga taaattagct gttataaacg aattattaga aaaagttgat
    16561 aatctaataa taggtggagg aatggcttat acattcttaa aagctcaggg ttatgaagta
    16621 ggaacttctt tacttgaaat agataaagtt gaatatgcta aagaaatgat ggaaaaagct
    16681 aagaataagg gtgttaactt attattacca gtagacgttg ttatggcaga ccactttgca
    16741 ccagatgcta ctccaatagt aacagaagat gctaatgtaa aagaagatta catgggacta
    16801 gatatgggac ctaagactat agcaaacttt gtaaaaacta taaaagaatc aaaaactgta
    16861 gtatggaatg ggccaatggg agtatttgaa tttgaaaact ttgcaaatgg tacattatca
    16921 gtagctagag ctatggcaga gttgactgat gcaactactg taattggcgg tggagatagt
    16981 gcagcagcag ttaatcaatt aggatttggt gacaagatga ctcacgtatc aacaggtgga
    17041 ggagcatcac tagaattttt agaaggtaaa gaattaccag gtattgcagc attagataat
    17101 aaataaacta atatatagag gagattgtaa aatgagaaaa cctataattg caggaaactg
    17161 gaaaatgcat aaaacaataa aagaagcttt agaatttgta aatgaagtta aagataaagt
    17221 aaattctgat aaagtagaag ctgttatatg tgcaccattt actttattaa aggatttaaa
    17281 agaagctact aagggtacaa atataaagat aggtgctcaa aatatgcact ttgaagaaaa
    17341 aggagcattt acaggagaag tttcacctct tatgttaaaa gaaatagata tggactatgt
    17401 tgtaatagga cattcagaaa gaagacaata cttcaatgaa actgatgaaa ctgtaaacaa
    17461 gaaagtttta aaagcattag aagtaggaat agacccaata ttatgtgttg gagaaacttt
    17521 agaacaaaga gaagctggaa aaactaaaga tgtttgtaaa gttcaagttg aaaaagcttt
    17581 agaaaatgtt cttaaagatg atttagcaaa agtagttgtt gcatatgagc caatctgggc
    17641 tattggaact ggtaaaactg caactgctga agatgctaat gacgttatat cttatataag
    17701 agaagttata aaaggattat atggagaatt agctaatgaa gttagaatac aatacggtgg
    17761 aagtgttaaa ccttcaaatg tagccgaaat aatgggtcaa agtgatatag atggagcttt
    17821 agtaggtgga gctagtttag ctagtaatga ttacctagac cttgttaatt tctaaggagt
    17881 gaaatgatga aaaaaccagt tgctttaatt attatggacg ggtttggata caataaggat
    17941 gttaagggaa atgcaattgc tgagtccaaa actcctaatt tagatagaat aaaaaaagag
    18001 tatccaaaca cattgataaa tgctagtggt cttgatgttg gtctacctga tggtcaaatg
    18061 ggaaattcag aagttggaca cactaacata ggtgcaggta gaatagtata tcaagattta
    18121 actagaataa caaaatctat aaaagatggt gactttttta ctaataaagt tttatgtgaa
    18181 gcaatggata atgcaaaaga aaactctctt catgtaatgg gacttttgtc tgatggagga
    18241 gtacattcac atatagacca tcttaaggct ataatcaaaa tggcaaaaga taaaggcgta
    18301 caaaaggtgt atgttcacgc atttactgat ggtagagata ctgaccctca aagtgcatta
    18361 gaatatgcca aagaagtaca agctagtatg gatgaaattg gagtaggtga atttgctaca
    18421 gtttctggta gatactacgc tatggataga gataagagat gggaaagagt agaacttgca
    18481 tacaatgcca tggttagagg tattggtgaa aaagcaaatt ctattgagga agctattcaa
    18541 aactcttatg atgatggaaa aaatgatgag tttataatgc caacagttat tatgaaagat
    18601 gataaaccag ttggaagtat aaaggaaaat gattcaatta tattcttcaa ctttagacca
    18661 gatagagcta gacaaataac tagagcttta gtttgtgaag agtttgatgg atttaaaaga
    18721 gaagatatta aaaacttctt tgtatgctta acagagtacg atataactat agagaatgtg
    18781 catatagcat ttggacctca atctttagcc aacactctag gtgagtatct agctaaaaat
    18841 ggaaaaactc aacttagagc tgctgaaact gaaaagtatg cacatgtaac tttcttcttc
    18901 aatggtggag tagaagagcc aaataaaggt gaagaaagac tattgatacc ttctccaaag
    18961 gttgcaactt atgatttaaa accagaaatg tcagcatatg aattgacaga taaagcttta
    19021 gataaattag gagaagataa gtttgacttt atagtattaa actttgcaaa tcctgatatg
    19081 gtaggtcaca caggaagtat agaagcggct ataaaggctg ttgaaactgt agatacttgt
    19141 gtaggaagat tgatagataa aatagtagag ttaggtggaa gtgcaataat aactgctgac
    19201 cacggaaatg ctgaatatat gttagaccca gaaacaggaa aaactgtaac ggctcactct
    19261 attaatccag taccatttat tgtagttggt caagaatatg aaagtgcaaa attattggat
    19321 ggaggaagat tatctgatat agcacctact attttagata tgatgaagtt agaaaagcca
    19381 gaagaaatga ctggtcattc acttatttca aaatagtcga ttctgataag tagataataa
    19441 tatataaact acaatataaa tttaaaaata atgcagttta ggtttatttt gtaaagttta
    19501 gactttatga cataaggttt aaattttgta agttctggat attgtaaggt gtagatgaag
    19561 atatatttta taaaatttac aagatagaca atttataaaa taaaaaatta caatatagat
    19621 aataatgtaa ataatgatat aaataataac aagatagaca ataacaatat aaataataat
    19681 aattaatata tattatataa gcaaaaattt tagaatcatt agttgtacat tttatgttta
    19741 atgttttaaa tctagttata atatttgtgt atttaagatt ggttttggat tttagacaag
    19801 tttttttata
//
LOCUS       DAGSWI010000070        19631 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.070, whole genome shotgun sequence.
ACCESSION   DAGSWI010000070 DAGSWI010000000
VERSION     DAGSWI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19631
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.070"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            366..1643
                     /gene="hflX"
                     /locus_tag="MJF80_002299"
     CDS             366..1643
                     /gene="hflX"
                     /locus_tag="MJF80_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBZ0280956.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     TSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1876..2373
                     /locus_tag="MJF80_002300"
     CDS             1876..2373
                     /locus_tag="MJF80_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBZ0280957.1"
                     /translation="MDAESLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIEIEQNEKDSRAI
                     CLVTKDRVEEYSKKVRGMQEKVLQLLFEDFTEKEAEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2422..2946
                     /locus_tag="MJF80_002301"
     CDS             2422..2946
                     /locus_tag="MJF80_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBZ0280958.1"
                     /translation="MKSVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            3258..4622
                     /locus_tag="MJF80_002302"
     CDS             3258..4622
                     /locus_tag="MJF80_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBZ0280959.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            4761..5927
                     /locus_tag="MJF80_002303"
     CDS             4761..5927
                     /locus_tag="MJF80_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBZ0280960.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            6044..6826
                     /locus_tag="MJF80_002304"
     CDS             6044..6826
                     /locus_tag="MJF80_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBZ0280961.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(6945..7598)
                     /locus_tag="MJF80_002305"
     CDS             complement(6945..7598)
                     /locus_tag="MJF80_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0280962.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(7622..8986)
                     /locus_tag="MJF80_002306"
     CDS             complement(7622..8986)
                     /locus_tag="MJF80_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBZ0280963.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            9644..11074
                     /locus_tag="MJF80_002307"
     CDS             9644..11074
                     /locus_tag="MJF80_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0280964.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            11177..12280
                     /locus_tag="MJF80_002308"
     CDS             11177..12280
                     /locus_tag="MJF80_002308"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBZ0280965.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            12295..12531
                     /locus_tag="MJF80_002309"
     CDS             12295..12531
                     /locus_tag="MJF80_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBZ0280966.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            12548..13597
                     /gene="vorB"
                     /locus_tag="MJF80_002310"
     CDS             12548..13597
                     /gene="vorB"
                     /locus_tag="MJF80_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBZ0280967.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            13599..14339
                     /locus_tag="MJF80_002311"
     CDS             13599..14339
                     /locus_tag="MJF80_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBZ0280968.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            14342..14881
                     /locus_tag="MJF80_002312"
     CDS             14342..14881
                     /locus_tag="MJF80_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBZ0280969.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            15005..17104
                     /locus_tag="MJF80_002313"
     CDS             15005..17104
                     /locus_tag="MJF80_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBZ0280970.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMEKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(17371..17886)
                     /locus_tag="MJF80_002314"
     CDS             complement(17371..17886)
                     /locus_tag="MJF80_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBZ0280971.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(17978..19189)
                     /locus_tag="MJF80_002315"
     CDS             complement(17978..19189)
                     /locus_tag="MJF80_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBZ0280972.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKFLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         7540 a         2286 c         3359 g         6446 t
ORIGIN      
        1 ctacttaaat ttaagaatag ggttaaatat aaaatgataa ttacatataa gttaattaaa
       61 ctaatgtatt tttaatatat atttagaatt ttaatccttc ttatgatata agtagcttgt
      121 tttgtattac ttagatttgt aaggagtttt tttattgaaa aaactgcata atatatttgg
      181 gaggaggtgt aagaaagtga ttttttatat aagattgaga aaataatgca cgattatggt
      241 tattataata gtaaataaga agtgtcattt aaggataaga taaaaagcaa aattcttgat
      301 gaatttaatt atagagccat aatctcgtgc tatagattaa ataaaaaata gcagataagg
      361 agaatatgga tatggcgaaa aaaggaataa cagtaggatt aaacataaat aataaatctg
      421 aggactttaa tgaactaatg attgaacttg aaaacttatg ttcagcatgt gatattgatg
      481 ttgtagggag tataactcaa aatgcaaagc aggttaatag agcattttac attgggacag
      541 gtaaagttga ggaaatatta aacttaataa agaaagaaaa tatagagata gttatatttt
      601 acaatgaact ttctacatca caactaaaaa atcttgaaga aaagcttaat tgtgaaatta
      661 tagatagaac ggcattgata cttgatatat ttgcacaaag ggcaaaaact agagaagcta
      721 aacttcaagt agaagtagca agtttaaaat atatgttacc aagacttata ggttcaaatg
      781 aaaatttagg acgtcaaagt ggtggagttg gtactaaaaa tagaggttct ggtgagaaaa
      841 aattagaact tgaccgtaga agaatagaag aaaagataac atctttaaat agggagttgg
      901 atgatttaaa attccaaaga gaaactcaaa gaagtatgag aagaaaatca aatcttccaa
      961 atgtagctct agtcgggtat acaaatgctg gtaaatcttc aataatgaat aaactagtag
     1021 atatatttaa aaactctgaa gaaaagaaag tgtttgagaa aaacatgtta tttgctacac
     1081 ttgaaacttc tgtcagaaat atagttcttg caaataacaa agaattttta ttatctgata
     1141 ctgttggttt tgtaagtaat ctaccacatg acttagtaaa agcatttagg tcaacattag
     1201 aagaagcatg tgaggcagac gtactgttac atgttattga tatttcaaat ccaagctata
     1261 aaagtcatat taaagtgaca gaagatactt taaaacaaat aggtgcagat ggtataccta
     1321 tgatacatgt gtataataaa attgatttaa tagatgtaga agtattggat agaattttag
     1381 atagtatcga taaagaaggt atatttgttt cagttaaaaa agatataaat atagataaaa
     1441 tgataaaatg tatttgtgat agcatattta aagattatgt aagatgtaag ttcttaattc
     1501 catatgataa aggtcatgta gtttcttatt ttaacgaaaa tacgagcatt ataaatacag
     1561 aatacagaga agatggagct attttagatg tagaatgtag caatatagaa tataataagt
     1621 ataaaaagta tgctttagag taaattttga aaaacttatt atagcctaaa atatatttaa
     1681 tttataattt attataaatt gattgtaaat atgattttat gaaacggtat agccataatg
     1741 ctatgccgtt ttgccgttta taataaaaat aaatttattg acataatagt aactaattac
     1801 tataatataa agtaattagt tactatttaa aattatcaaa aaattaaaat ctaagataaa
     1861 gagaagtgta taataatgga tgcagaaagt ttaatgaaaa aatatatggt agatgatgat
     1921 agtagaaaac aagctatatt tgcaagtata tttgtaatgc aaaataaact tcaaactaca
     1981 tgtgataaat tagacccaga attaacaatg aaacaatggt tacttttagc tattgcggac
     2041 tctgtagatg aaactcttac attaaccaaa cttggagaat taatggggtg ttcaagacaa
     2101 aatgtaaaaa aactagctat tgctttagaa aagaaaggtt ttatagaaat tgagcaaaat
     2161 gaaaaagatt caagagctat ctgtcttgtg acaaaagata gagtggaaga gtattctaaa
     2221 aaagtgagag gtatgcagga aaaagtatta cagcttttat ttgaagattt tacagagaaa
     2281 gaagcggaac agttattttt tggaattatg aaattaagta taggaattga aaatgtgaaa
     2341 aattatgtag aaaataatga cgttggagaa taagcaagta tcaaatcaac tttgtatgac
     2401 atcagagagg ggagaattta tatgaaaagt gtagtagtga tttataagag taagtatggt
     2461 tcaacaaaaa aatatgctaa atggctttca gaattattgt cttgtgatat ttttgaaact
     2521 aagaatatta atatagaaaa ggttatgaag tatgatacga ttattttagg aggaggtctt
     2581 tatgcttgta atatagcggg aatttctttc ttaaaaaaaa attataaaga attgcagaaa
     2641 aaaaatttag tagtatttgg tgtaggagct gctccatatg atgatgaagt aataaagaaa
     2701 ttaagagaag taaattttaa ggatgaatta aacaaaattc cttgctttta ttgtcgtggg
     2761 gcatgggatg agacaaaaat gtcttttata gattatactc tttgtagttt ttttaaaaaa
     2821 tctatagcta aaaaagaacg tcaaggtctt aaaccagcag aagcaccttt attgtctgga
     2881 gaagcctttg attggtgtaa taaagaaaat ttaactccaa tacttgatta tataagagaa
     2941 ctttaagagg tatatatttg taattattta ttatctaaag ttagatttca tagaaactta
     3001 ctagacatta tttataaaat aatattatta aagatggata atttaattgt ttcaataagg
     3061 ttataatttt aaaaaatgtt tattttaaaa taaactattt tataagctta cttatactta
     3121 ataaggggct ttttttattg gaagatttat gttatattga gatgaattta ttatgatata
     3181 aaaaataatg ttaggataaa tttaaatctt tttctaaaaa tgtattagta taattcttaa
     3241 agaaaggatt tttaacaatg gatataaaag agttagttaa aatgcaaaga aaatattata
     3301 atacaggtaa aacaagagat atatcgtttc gtatagaaca gttgaagaaa cttaagttag
     3361 ttgtatcaca aaatgaagaa aaaatacttt tagcactaaa gaaagattta aataaatcgg
     3421 attttgaagg ttttatgaca gaggtaggca tgttttatag tgaattaaac tttgctataa
     3481 agaatataag aaaatggagc aaaataaaaa gagttaaatc aagtatggtt aattttccat
     3541 ctatatccaa agttgttcca caaccttatg gtgttactct cataatgtca ccttggaatt
     3601 atccatttca attagcactt ataccattag tttggtcatt agcagcaggg aattgtgtta
     3661 tattaaaacc ttctgaatat tctgtgtcaa catctagtgt agttaaagac atagttgaag
     3721 atactttttc aaaagaatat gttgcagttg ttcaaggaag tcaagaagaa agtgaaaagt
     3781 tactattgga aagatttgat tatatatttt ttacaggaag tactaatgtt ggtaaaataa
     3841 tcatgaaatc agcatcagag catttaactc ctattacttt agaacttgga ggaaaaagtc
     3901 cttgtatcat tttaaaagat gctaatatag accttactgc caaaagactt acttggggaa
     3961 aactaataaa tgcaggacaa acctgcgtgg ctccagatta tgttttagtg catgaagata
     4021 gaaaaaatga gttaatagag aaaataaaat attatactaa taagtatttt ggggataatg
     4081 catgtaataa tgagcaattt cctaaaatca taaatcagag acattttaat cggatattat
     4141 ctcttattga taaagataag atagtctatg gtggaaatta taataaggag acattaaaga
     4201 tagagccaac tatagtagac aatgttaatt gggatgataa tataatgaag gaagaaatat
     4261 ttgggcctat atttcccatt ttaacatata aagatttaga tgaagtaata caaaaaatta
     4321 tacaaatgcc aaatccattg gcactttata tttttacaaa aaataagtac ctagaaaata
     4381 aacttttaga aatgattcca gcaggcggat gttgtatcaa tgatacagtt actcatatag
     4441 ctacgaatta cttacctttt ggtggtattg gtgaaagtgg tatgggctca tatcatggaa
     4501 aagctggatt cgatacattt actcactata agagtgtgtt aaaaaaatta aatttggatg
     4561 tccctattag atatgctcca tacgataata aattaattaa ggttcttaaa aagataatgt
     4621 aggttatatt gtagaataat aaatataaat aaaattgagt atttgtattc tgaatatatt
     4681 ttatttttaa tttggtatat ttgttataaa agtggtgatt ttgtgataaa atttaattat
     4741 atagaaataa ggaggaaaaa atgaaaatta attggaatac aaaatacaca acaatagcaa
     4801 tttatacatt tataatagct gctagtagta ttatatttta tctagtatca tcacaaattg
     4861 atgtattttc taataattta gatgctatat ttacaacatt acaacctttt ataataggat
     4921 ttgctattgc atatcttctt aattttattt taaagtttta tgaagacaga atttttataa
     4981 aaagtgagaa attaaaaaaa ctaaaacaaa gttcaaaaag aggcttgggt cttttattaa
     5041 catatgcaac agctacattg atactttact tatttatgca tttcgtattg ccacaagtaa
     5101 ttgagagtat tgttggacta gcaaatgata tacctatgta tgtaaacaat gcaactaagc
     5161 ttattgataa attaatgact gatttaaatc ttgatgagca atactttaat ttggcagttg
     5221 ataagtggaa tgaatttgtt acatatatta taaagtttgt aacagattta atcccaatat
     5281 taggaagtat gttaaagaat gtagcctcta gtatatggaa tattgtacta ggattaattg
     5341 tttcagtgta cttattaatt gataaagaaa aattctatgg attaagcaaa aaaataactt
     5401 atgcagtatt tacagaaaaa caagctgcca gaattttaga attaactcat agaagtaact
     5461 atacatttgg aagattttta ggtggcaaaa tattagattc attcattata ggtattctaa
     5521 catttgtaat acttacgtta gttaaaatgc catatacatt attaatatca gttataattg
     5581 gtattacaaa tattatccct ttctttggac ctttatttgg agctatacca tctacgctaa
     5641 tagtgatgtt tgtatctcct ataaaagctt tctggttatt gttgattata ttaataatac
     5701 aacagataga tggaaatatt ataggaccta aaatacttgg agattcaatt ggtatatctg
     5761 cattttggat tttgttctcc ttattggttg caggaaaatt attaggtttt attggaatgg
     5821 ttataggagt tccaatgttt gcagtaattt attctataat taaggatatt gttgaaagta
     5881 aattggacaa aaagggatta ccgacagata cttccgatta tatgtagcta aaaaatatta
     5941 tgtataatta aaaatatata aattaagcaa tatttaaagc actgagaaag ttaaactctt
     6001 ggtgcttttt agatataaaa ttattataga agaggtgttt tttgtggcat ttatagaatg
     6061 taaatttaaa tctaaatata ctggaggaga aactgatata attgtaattt tgcctgtagc
     6121 aaatggaaaa gaacttttta gtggtaaaga gatatatgat tttgaaggta aatttaaaac
     6181 attatactta tttcatggtt tagaagatga ccaaagctca tggattagat atactaatgt
     6241 agagcattat gctaaaaata aaaatatagc cctagtaata ccaagagtag aaactagttt
     6301 ttatactgat atggcgtatg gacaccaata ttttactttt gtaagcgagg aattacctaa
     6361 atttgttaga gctatatttc ctttatcaga taaaagggaa gataactttg tagctggtat
     6421 gtctatgggg ggatatggag ctttaaaatt agcgctttca aaaccaaaag agttttcggc
     6481 agttgcaagt ttttctggtt cgcctgatat gattcatgag ttagaaaatc aacaaataga
     6541 aaccaatgca gacattctat ttcacgattt tggtagacca gaagacgcta gaaatagtga
     6601 gaatgattta ctttacatac ttaagaacct taaagaaaga aatgaagata ttcctaaaat
     6661 ttatcaattt tgtggtgata gagatttttt atataaaaat aatcagatgt ttaaaaaata
     6721 tgcagattct ctgggtgtag atgttgaata tgaagagtgt gtaggggggc atgaatggcg
     6781 tctttggaat ttatggataa agaaatttat agatataatt ggatagtaca cttatggtat
     6841 taacaggtat actataaaat atctttaaat atgataaaga ctgaaatcct aaaatcaaat
     6901 tatgatttta aaatttcagt ctctaagaat ttaataaaat tttattaagt tcttacacga
     6961 gttattattg gagtaaaatt gtcaactata tctatatagt atttactcaa ttcctctaaa
     7021 atctcttcgc ataaatccgt actatgacca agattaataa gaaactgttt tgtaaaatgc
     7081 tttattacag cttgagaaga tatatcgtac ttgtgtgaag tataaactct aattatttca
     7141 ggtttcatag caagtatacc aatacaagaa agtattaatt catcatgttc tatagattct
     7201 ttatcattaa catatctttt catgaccatt tcggttacac ttctttgata gcttaaaata
     7261 gcttcttcat aagctatatc caaataaaaa tttctatatg cttcgttttt tagaaggaaa
     7321 caaaggtcag cattcatagc aagaaagaac atcatcatag taccttcttc atggtagtta
     7381 tagattaaag actgcatttc atttcttata tcttcatata ttatagctcc tatttcgctt
     7441 ttacttgaga agtaatagct tattagacct aaatttatat tagctctttc tgcaatttgc
     7501 ctagttgttg tcttatgata accttgctca aaaaataatt ctctagcagc acttataata
     7561 ctagcttttg tttctttgcc cttttctgat tttgacatat ttactgtgta tacctctctt
     7621 ttcaatcttt ttttgaaaat ttgctaacta taaatattgt aatagaacca cctattatag
     7681 gaaccaaaat tctgtcaaat acagtaagaa taccaatcat atttaataat gccaatataa
     7741 cacttactat aacagcatag atagctcctt tttttgtagc acctttccag caaagaccaa
     7801 ttacaaatgg tgcaaatatt acacctgtac ataatggagc tggccaaaac aataattcta
     7861 ataaactatc aaatttaagt gctatgattg cacctattat agctgtcaaa gctgatgcaa
     7921 tttgagaata ctttagagta ttttttaaat cattaaattc tatattagga tgtaataatt
     7981 tgttataaat atctcttgat ataatagcac tcattgaggt cattaatgaa tcggcagtag
     8041 acattatggc agccaatata gcagctaaga ataatacaga tattaaaggt ggcaaagtag
     8101 tcgatatcat ttgaggtaaa acctgaacag atgatatatt tggaaataaa ctaccaccaa
     8161 tagctccaat tattcctata gggaaagcaa gaaataatag tataaatcct gcaattacac
     8221 tacttttaac tgcattttta gcattatcag cacattgata tcttaaaaaa ctttcataag
     8281 aaaatattga gcctaaaact aatggtaaaa ctgtatacat aaaatcacta atcgatttat
     8341 ttcctggaaa gaatggttct tttaccattg attgtaacat aagtaaaggg ttgtcgccaa
     8401 ctaattttaa gcttatataa gctactattg gcaaaaacac tatacaaata gcaagttgaa
     8461 taaatgctga tactgcaact ccccacattc ctgatgtata agtatatata atgataacta
     8521 taactcctat aattacagct atatcataag gaataccaaa tgcagtagat acagaaccaa
     8581 aagaaatcaa ttgactaccc ataatagcta gtatagatat tggtgttaca agactttgat
     8641 aagctcttaa aaatacacta tgaccatatc tagtttcaat ataatcagca ggtgttatat
     8701 cacaagataa gcttcttaat tttcctgcga aaaatgcatt tataaagcaa gctaaagctg
     8761 cacctatagt agaatacatt ccaacccaaa gtcctctttc agctccccat tgaactccac
     8821 caactaagca ccctcctcca aaagatgtag cagcaatggt tgcagctagt ataggcaaag
     8881 gcagatttct acctgcgatt aggaaatcgg atgctgtttt aacttttttt gaaaaataaa
     8941 aaccaatcca tatgtatatt gcacacgtaa caattaaaag taacatagtc ttttctcctt
     9001 tataaaatat tttaataaaa tatacaaaat atatttaaaa tatattttat ataaaagtag
     9061 tacgatattt gttttattgg ttttagatta atatagctat aataatttat gatattagat
     9121 ggaaattacg taaaaataga aaattgaaaa ttctgaaatt tttgcatatt gatatttatc
     9181 tcatagattc ttttttgtaa ttgtaacacg cttttatttt taaatcaata gtacatgtat
     9241 aaaaataaaa ggaatttaaa taagaaaaag atatttttaa tatctggatg tagtatagaa
     9301 atatcttgaa taaattattc ttgaataaaa ttataaaaaa ttaaaatcac tagaaatatg
     9361 ataaaataat ttaaaatacg catttcaatg acatttcaaa acaagaaaat aataaaaaac
     9421 aaacctaaat taagtctaaa tagcttttta gttaatttta gtagtaaaaa ataaaactta
     9481 aaaagtgttt gacaattatg aattttgaga atataattaa attaaaatta tacacatata
     9541 aaaatatact cctgaatcaa gaatgagata tttaagattc aaaatatata ttattatacg
     9601 tgaataataa aaagtaagta aattttataa gggaggggta gatgtgagta gtcaagaaag
     9661 caaaccaaaa ggaaatctta tatcaaattt tagagatgat atgagagacc ctaagaaaag
     9721 agcaatatac acatattcaa gttctataaa tttagcgtac aattttatag gttactttgt
     9781 atcaactgtt acaaatattt ttttaactga tgtggcaaaa cttggtgtaa ttatggcagg
     9841 tggtatgcaa attatacaat ctttcataaa aattgctgta gctcctataa caggtacagt
     9901 atttgacaaa caaccattca aaaaaggaaa gtattatcca tggcttagat atgcaccagc
     9961 agcgttagca ttaacatatg gaatattttt catagttcca ttaatgaaaa taagtccagc
    10021 attactttta cctttgatga cagctttatt attaatagca tcttgtgctc agcaagtagt
    10081 tttcactata atttatgcta tttaccctgt agttgctaag acacctagag accgagttat
    10141 aggctcaact actacgaata tatttaaaga agttggtaaa ttcttagtag gtttttcata
    10201 tccgttatta ttagttttat ttgcagatgt attaggtgga gaaggaatgg gttatttagg
    10261 tacctactta ttctttgcag caggatgcct tttagtattt tggttttctt caacagagat
    10321 aataaaatct ggagcagaag aagaagttct tcaaaaagaa gagaagccaa agattaaagc
    10381 aagtgaaatg gcaaaagctt tattcactaa tgtaccactt atgatggcat tttgtttaga
    10441 atttcttata tgtataagat ctataggatt aggacctttg gctccatatt attttaaata
    10501 tgtaatagaa gatgaacgtg gattagcagt atttttatca atcatgcctt tggtaagtgt
    10561 agcattcatg tttttcgcac cagtgtttat aaaaatctgt agagagcaaa aactagcttc
    10621 aataatttct ttttctatat gtgcactatg ccacttattg gttgcatgga ctccttgggg
    10681 gaaaactact ttaggagtaa ctatgttgtt ggcaattggt ggaggttttt caaatgtagt
    10741 atcaataata aatcttaatt tctttgccgg ttcttgtgat tatgggcatt ggaaatcagg
    10801 aaaagactta cctggcttaa gtatgtcact atatccagtt gcaatacaag taggagtatt
    10861 acttgcaact actataagaa cagttttaat gaactcaatg ggatatcaag ctgatatggt
    10921 agtcacagaa gcagttaaat caggttttat aaatatgatt tcttattcta tggcaatacc
    10981 tttaataata gctgttgtta tagcaatact atatccagta agtgacaaga aattaaacca
    11041 aataagagaa gagttaaatc aaagaaatgc ttagttaaaa gaaagttgag tagatggtta
    11101 atttaagcta ttaaagtaag tagacgcaat agcgtttata taaatgaaaa tacatataca
    11161 gagggggctt ttaaaaatga tagaaaaatt attgagaaat accgtaaaag aattacatca
    11221 atatgttcca ggtgaaccaa tagaaaaagt taaggaaaaa tatggtgtaa aagaaataat
    11281 aaaattagct tcaaatgaaa atcctctagg accatcacca aaagcaatag aagctatgat
    11341 tgaaatgtta aaacaaggtc aactttatcc agagccagaa gctaacgaac ttagaagaaa
    11401 attagctgaa aagctagatt taaaaccaga aaattttatt gttgctaatg gtgcagataa
    11461 cgttatcact ctaataggtg aggcttttat aaatagagga gatgaagtta tatattgtaa
    11521 cccaacattt ccatcatata gaactgcaac tataaaaaat gaagggatac cagtagaagt
    11581 acctttaaca gaagattata agtatgattt acaaggtata ttagataaga ttacagataa
    11641 aacaaaatta atatgtgtat gtaaccctaa taatccaaca ggaacaattg tggatgataa
    11701 agagttagaa gaatttttaa agaaagtccc tgaaaatatt ataactatat tggatgaagc
    11761 atatatagaa tttttaacag ttcctaatta tgtggatgga cttaagtatg taagggaaaa
    11821 ttataacgtt atagtaacaa gaactttctc taaaatatat ggtcttgcag ggcttagagt
    11881 aggatatggt attgctaaag atgagataat aagaacttta tttacagtaa aagagccttt
    11941 ctctgcaaat cgtgttgcta taagtggagc aacagcagca cttgatgatg aagagtttat
    12001 aaaagaaaca tatgaattaa atagagttgg aatggcttac tttaaagaag agtttacaaa
    12061 aatgggattt gatgttgtag attcacaatc aaatttcctt tatgtagata tgaaaacaga
    12121 tatacctaag ttatttgaag acttgaaaaa aagagggttt gtaataagac caagtgctac
    12181 acatgcacgt gtaagtattg gaactatgga agaaaatagg aaatttgtag cagttttaaa
    12241 ggaaatatta aatattaaag gagagccaag tattggataa tagatagaaa tattatgaaa
    12301 cggggtgtag atatgaactt aaaaataaac gcagagagat gtaaaagttg tgaatactgc
    12361 gttatatctt gtaaaaaagg agcattaaaa atatcaagta agataaataa agaaggttat
    12421 gctcatgtag aagttgatga gagtaaatgc atactttgtg gaatttgcta tcaggtgtgt
    12481 ccagataatg tttttgaaat aattaaagaa gaagcatgtc aagaggctta gaaggagttg
    12541 ggataatatg aaaaagatgt ggaaggggaa ccacgccatt gcagaagcag ccctaagagg
    12601 aggttgtgaa ttttatgcag gatatccaat tacaccacaa acagaggtaa tggaattttt
    12661 atcacataga atgtctgaac ttggaagaac ttttatacag tccgaaaatg agatggctgc
    12721 tatatatatg gtatatggag catatgcaag tggaatgagg tctatgacga gttcttcagg
    12781 accaggaata tctttaaaac aagaagggat ttcttactta tgtgcaaatc attacccttg
    12841 tgtaatcgta aatgtacaga gatgggggcc tggtcttggc tcattggact cagcacagac
    12901 agactatcta agagatacta gagggggagg aaatggagat tatcatttga tagtttatgc
    12961 accaaacagt attcaagaga ctgtagattt gatgtataac tcatttgatg tagcggagaa
    13021 gtacagagtg cctgtcgaaa ttttgacaga agcagcttta ggtcaaatga tggaaccagt
    13081 agaatttcca gagtttaaga agagagaagg agatttaggt tggacttatg atggaagtaa
    13141 tagagaccat gcaaaggttg cagataacca aaagccaact ttttgtatgg agaaacgtat
    13201 gagaataact gaaaatgaac aacaatggga agattatcaa gtagaagatg cagaatatgt
    13261 gtttgtagcg tttggtatac catcaagaac aactatgaat gctgttagaa gacttagaga
    13321 acaaggtgaa aaagttggaa taataagacc aataacagta tggcctttcc catataaagc
    13381 atttgaaaag gtaagtaaag atgtaaaagg atttatctca gtagaatcaa cagacacagg
    13441 tcaattagta gaagatgttg ctttagcctc aaagaaagta tgtaaagaaa atgtacctgt
    13501 gtatggttta tttagtggaa atcatatacc aaaaacattg caagtaatgg acacttatag
    13561 caaaataaaa tctggcgaaa tcaaggaggt gttctaatat ggctacagaa aaattaacac
    13621 ctagcgttgt ttcaatacca aacaagtttt gcccaggttg tggtcatgga atagtaaatc
    13681 gtataatagc agaggtaata gaagaacatg gatatgaaaa aaatcatgta ttaacattag
    13741 gagttggttg tgtctgcaat atgaatttta gctggaatgg tgataagatg caaactgcac
    13801 atggtagagc ttcatctaca gctataggag ttaaagtagc acttccagat acattagtaa
    13861 tgacttacca aggtgatgga gatgcttatg ttataggtct ttctgaaact ttaaatacag
    13921 catatagaaa tcataatgtg actgtatttg taataaacaa taataatttt gcaatgactg
    13981 gtggacaaat gtcatggacc actatgccgg gtcaagtcac aacaacaagt gtaaatggaa
    14041 gagatataaa gactactggt agtccactga aagttcctga gatggtggca aacttctttg
    14101 atgtagcata tgtagcaaga ggttcagttc atagcccaaa ggaaataatt aatctaaaaa
    14161 aatatataaa aaatgcaata gaagctcaat taaatggaga gggatattcc ttagttgaaa
    14221 tattagcacc ttgcccaact aactggggtg tatcactaga gaaatctatc aaatggatgg
    14281 aagaagaaat agtgccatat tatgctcttg gcgaatttaa gcaaagggat ggtgaatagt
    14341 tatggaaaaa gagctagtat ttgcagggtt tggtggtcaa ggagttttaa ctcttggtct
    14401 gattgtagct gagtcagcac ttgaattggg gaaacaagta acttggatgc ctgcatatgg
    14461 tccaactatg agaggtggaa aagcctatag tgtagtaaag ttttctgatg agagtatagg
    14521 tgggcctgat atggaagaaa tagatgtttt agtagcaatg aataaaccat ctttagatta
    14581 tataaattta gttaaagaag gtggaacagt agttattaat acatctgcaa tagatgagaa
    14641 tgtaacatta agagaagata taagtgtagt taaaataaat tgtcaggaat tagctcaaaa
    14701 agtaaacaat ccaaaagcag caaatatagt agtacttgga gcattaattg ctaaaactgg
    14761 gttatttgaa aaagaattag ctttaaaaac tatgtgtgat ttctttgagg aaaaaggaaa
    14821 aggaaagttt aatgttcaaa atgaagcagc attcatggaa ggctataata acgccatttg
    14881 ataaattacc cttatcttaa gtgatttatc tcaagattta ataatcttta gtgccaagag
    14941 aaacttcatc ttgagataaa attcacttaa aatcacaact ataaacatat aggagcgtga
    15001 atttatgagt ttggaaaaac ttattagacc aaaaagtatt gctatagtag gagtgactga
    15061 taaacttggt tttggaagaa gtgcagcact tagtatagta aaaagtaagg aaacagatag
    15121 agtatattat gttaatccca aaagagaaga attgtttggg agaaaatgtt ataagacgat
    15181 acaagaagtt ccagaagtag tagactgtgt agtagtatgt acgcctagaa atgtagttcc
    15241 atctgtatta aaagattctg gagagttagg aacaaaggca gcagtggtat atgctagtgg
    15301 atttgcagag gaaggtacag aggaaggtac agatttagaa aatcaactta tagaaatatc
    15361 aaatacatac gatatgaaaa tattgggtcc aaactgcatg gggttgctaa attgtataga
    15421 taaggttaat ctatgggcag gaggttctaa atgggattta gatactaaaa aacctggtat
    15481 aggaattgta ggtcaaagtg gatttataac tgctgaaatt gtttcaagtg attattttaa
    15541 tatatcttat ggtttttcaa caggaaatgg aaatatagtt acactagaag atgcagtaga
    15601 tttccttgta gatgatgatt atgcatcagt aatagcaatt taccttgaag gtttaaaaaa
    15661 tcctcaaaaa tttatagatg ctttaaaaag ggcagcacaa aaaagaaagc cagtaataat
    15721 attgaaatca ggaaggtcag aaaaaggtgc tatttctgct gcttctcata caggaaattt
    15781 agctggttcg agtaaagctt ttgaaagtat ttttgaaaaa tatggagtaa tttctgtgga
    15841 aaatttagaa cagtttatgt gtttagcaca agcttttagt gttttagatg gtaatttacc
    15901 tgcaaattct aattttgcag caataaactt ctcaggagga gaaaatacta tttgtgcaga
    15961 tttagcagaa gaaaatggtg tagaacttgc agaaatatct tctgaaacta aagaagaaat
    16021 ggaaaaatat cttccaggat ttgcaacacc taaaaatcca ctagatgcta caacagcatt
    16081 attccatgaa aaggatatga tagtaggttt attacatacc tttgaaaagg atgatagtgt
    16141 tggatttact atggtaggtg caaatataag agatgaagaa aatgaaatgc atgaaaccct
    16201 ttgtaaagct gtaagtgaag ctagagaaca aggattgaaa aaacctgtgt ttgcagttcc
    16261 tacacttgaa ggaaatagat atttagatta taggactaga ttagaagata atcaagtgcc
    16321 tataatgtca tcagttggaa ctacatttac ttgttttaat aaaatggcta agtttataga
    16381 ttatgattat tcaaaaagaa ctttagaatt taaagctgtt aagaaaagag agtcaaataa
    16441 tgtagtagca ttatcagaat tagatagtaa gattgagatg aaaaaatatg gagtaccagt
    16501 tccaggacaa ggtaatgcaa aaagtattga tgaattagat gaaatcttaa aatatattaa
    16561 gtatcctgtt gttttaaaaa taaattcttc tgaaatattg cacaaatctg atgttggagg
    16621 agttaaaata ggaattaaga atagagatga ggcagtagat gcatataatg agattttaac
    16681 taatgtaaaa aaagcaaaac cagatgctaa tattgatggt atattagttc aagaaatggt
    16741 agaaagtggt atagaaatta ttattggtat aactaatgat gaccaatttg gaccaatgtt
    16801 attagtcggt ttaggaggag tatttgtaga ggtatttaaa gatactacat tatatccatt
    16861 acctataaat catgatgaag ctattatgat gcttaaaaaa ctaaaatcat ttaagctttt
    16921 aaatggctat agaggaagtg agccatgtga tattgatgct cttgcagata tgatggtcaa
    16981 gttaggaaaa tatgcttatg aaaataagga tgaagtcaag gaaatagact taaatccagt
    17041 gtttgtatat cctaaaggaa aaggcgtatg tgcagtagat gccttaatag ttaaatataa
    17101 atagtttata agttttggta ttcaaccata attaaagcac caactgaaaa tcacattttg
    17161 taagttagat agtgtgattt tcagtcaccc caaatttaat ttaaaaatat tttaaaatga
    17221 aagtatagtt ttaagtttca ttgtgaaata gcactgtata aacaataaaa tactacgtat
    17281 aaaacgaata ataaaaggtg ttttaatgat aagaatcaat cttgattttc attaaaacac
    17341 cttctttatt atattaaaaa tttttaaacg ttatattgtc acaggtgggt ttgttggagc
    17401 tgcatatgta cgattagcaa attcttggtc aacaaagaat agcccaccct ctccagcaag
    17461 tttaacacgt tttatattgt cttcacaatt tgtttcttct tctgcttgtt cagatataaa
    17521 ccaaagtaag aattgagaag ttctaaagtc tgattcagag tttgctacag ctgctaagtt
    17581 gtttattaaa gctgtaacct ttttttcatg agctaacgta cgctcaagaa tatccattgc
    17641 agaagtaaag tctgaatctg gcattgggat agcatcaagc tgaacctttc ctcctacatt
    17701 atgtaagtat ttgtaaaaaa acatagcgtg gtctgtctct tctttatatt gaacataaaa
    17761 ccaattagaa aatcctttaa gtccttctgc ttcaaggtaa gaagacatag aaagataaag
    17821 atacgcagaa tataattcat ggttaatctg ctcattaagt aatttttcca ttttagctga
    17881 aatcattttt atacctccaa attttaaatg ctcttataaa atataataat attaaatgga
    17941 aaaatattca ataaaagtgt ttaaatattt tacatattta ataaaaattt atactaccat
    18001 taaaaaaatt ataacttaca atggctatag agccaattaa tggtgcattt gctccaaaat
    18061 ttgaatgcct tacacttatt tttttatatt tagaaaaact tgctgaagca gtcaattttt
    18121 ttaataatgt attttctata aatgtaccag gtatattaaa gtcatagcct attattatag
    18181 aactacaatc aatcaatgta agtgtattaa ctaatgcata tgatacgtat gtacaaaatt
    18241 catcaagcaa aaacatggca agactatctt tattatttgc ctcatctatt aacttgacta
    18301 aagaaaaatc tttacagtta attaatgggg agttaggata aatatggctc aatgattcaa
    18361 ttcttttaat tagattactg acacttgtat aataatctaa acatccattg ttaccacaac
    18421 tgcacaatgg tccactaaaa ttaattgatg tatgtcctat ttctccactt tgtcctaggt
    18481 tgccagtatg aagtttattt tctagaacta gaccagctcc aataccattc attatgtgta
    18541 ggtatatata atttgatata tttttaccta aaccatacat tttttctgct aatgcacccg
    18601 aattagcatc atttatcaaa aatattggca aatcagacat ttcttttata aaatctacta
    18661 tatttaggtt tgatacattt ccaaaatctg ggggatttag tatggtacca tttatatcat
    18721 ctactggacc tattgaagaa ataccaattc caattatatc tctcttgttg attttcatta
    18781 ttttagtaaa cattcttttt attatatctt ttaaaacatc atttgataga aatttagggt
    18841 aagtttcaga aagttgtttt acaatattac cttttaaatc tgaaataatt accttacata
    18901 tatttcttct tattaggatt ccacaaatac aaggcgatat attagaaata tctaataaaa
    18961 taggtttacg acctgatgaa tgtgcattta aagttgatga ctctatttcc ctaatcatat
    19021 tttcattaat aagttcagta actagatttg ataaagtcat tttagtaagt cctgtagact
    19081 tagctaaatc aactctggac ataggtgtat ttgtagaaag taatttcaat attattaact
    19141 tatttttaat ttttacatct ttgatgttag aaccttgttt gttactcatc ataaatctcc
    19201 ttaaataaag ttaagtgttt tatttatttt caagtatact atcaccttat aatagagtaa
    19261 cattttttta cttgaaaata tcattaatta gaatatatca cattatatat cataaaacat
    19321 ataaaaaaat attagaatta aattaaaagt gaaaagtaga ttgatatata aatagtaaat
    19381 acttaattaa aaaaagataa tgatagcaat aattattgtt accaagaatc tactacttca
    19441 agtgttacct taggtattta agttgatagt ggtttgcacg taataaactt gatgtaatat
    19501 atattcttaa taatatcgat aaaagattta cttaaaaata atatagatta tgtaaacata
    19561 taatctattt attgtataca ataaaatttc attttgtaaa acagtgaatt aaatatgtaa
    19621 tatagatatc g
//
LOCUS       DAGSWI010000071        19498 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.071, whole genome shotgun sequence.
ACCESSION   DAGSWI010000071 DAGSWI010000000
VERSION     DAGSWI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19498
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.071"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            18..869
                     /locus_tag="MJF80_002316"
     CDS             18..869
                     /locus_tag="MJF80_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBZ0280973.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLLNRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1002..2615)
                     /gene="ggt"
                     /locus_tag="MJF80_002317"
     CDS             complement(1002..2615)
                     /gene="ggt"
                     /locus_tag="MJF80_002317"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBZ0280974.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2840..4777)
                     /locus_tag="MJF80_002318"
     CDS             complement(2840..4777)
                     /locus_tag="MJF80_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280975.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSILAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKRELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4764..5531)
                     /locus_tag="MJF80_002319"
     CDS             complement(4764..5531)
                     /locus_tag="MJF80_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280976.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5819..6493
                     /locus_tag="MJF80_002320"
     CDS             5819..6493
                     /locus_tag="MJF80_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0280977.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6517..7521
                     /locus_tag="MJF80_002321"
     CDS             6517..7521
                     /locus_tag="MJF80_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0280978.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7633..8862
                     /locus_tag="MJF80_002322"
     CDS             7633..8862
                     /locus_tag="MJF80_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBZ0280979.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIISEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8870..9544
                     /locus_tag="MJF80_002323"
     CDS             8870..9544
                     /locus_tag="MJF80_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0280980.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9547..10779
                     /locus_tag="MJF80_002324"
     CDS             9547..10779
                     /locus_tag="MJF80_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0280981.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11065..12330)
                     /locus_tag="MJF80_002325"
     CDS             complement(11065..12330)
                     /locus_tag="MJF80_002325"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBZ0280982.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12383..13531)
                     /locus_tag="MJF80_002326"
     CDS             complement(12383..13531)
                     /locus_tag="MJF80_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBZ0280983.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13622..14167)
                     /locus_tag="MJF80_002327"
     CDS             complement(13622..14167)
                     /locus_tag="MJF80_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBZ0280984.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(14390..15334)
                     /locus_tag="MJF80_002328"
     CDS             complement(14390..15334)
                     /locus_tag="MJF80_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBZ0280985.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            15720..16601
                     /locus_tag="MJF80_002329"
     CDS             15720..16601
                     /locus_tag="MJF80_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBZ0280986.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(16662..17870)
                     /locus_tag="MJF80_002330"
     CDS             complement(16662..17870)
                     /locus_tag="MJF80_002330"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBZ0280987.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(17943..18195)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(18347..19336)
                     /locus_tag="MJF80_002331"
     CDS             complement(18347..19336)
                     /locus_tag="MJF80_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBZ0280988.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
BASE COUNT         6838 a         3053 c         2394 g         7213 t
ORIGIN      
        1 cacaggaggt acattctttg aaaaacatta tttcagcaaa agaacttata tcaaaactag
       61 aaaaaaatga taatctagtt gtaatagatt gtagatttga tcttctcaat agaacttatg
      121 gtattgattc ttataaaaaa ggacacatca aaggcagttt tatcttagat atagacaagg
      181 atttatcatc tcctacaaaa gaacatggtg gtaaaaatcc tttacaagac cctttaatac
      241 taaaagaaaa attagaaaaa atgggtgttg ataatgacac tacgattgta acttatgatg
      301 atggagattt aaatggagct tgcagattat ttttccaatt aaaacatttg gggcttaaaa
      361 atgtctatgt actagatggg ggaataacat ctttcgtaaa ggaaggtgga gaattggaag
      421 agaaagtgaa tataccaagt tgtacaggta aggaaattag accaaatata aacaatgatt
      481 tagttgttcc aatggaatat gttaagtcta aattatacaa aaaagatact gtaataatag
      541 attgtcgtgc taatgagaga taccagggtt tagttgaacc tgcttactca aaagctggtc
      601 atataccaag tgcaaaaaat tatttttgta aggatttaat aaaaagtgac tttgaaaatg
      661 gctcacttaa agatatagaa tttttaaaaa aattctttaa agatttaaat aattatgatg
      721 aagtaatttt atcttgtggt tcagggatat ctgcttgtgt taacagtctt gctcttagag
      781 aactggatat acctcacaag atttatattg gtagctttag tgactggatt agttatgatg
      841 acaatgaaat caaaactggt caagaataag gctcataatc tatataaaat cagcatatga
      901 tagcttaagt tttgtaaagg cgtcagtcat tgtagacttt cgcctttact tattatttca
      961 tatactcttt aaatttatta tatatatttt agttgataat attaataaac tgcaatatga
     1021 ccatcacatc ttgattccgt cccacaaata taactttcct gttctgtttt aaatattatt
     1081 tgacctcgtc ccatgtcaac agtatcatga attactttta tttcatgacc cttttcaatt
     1141 aatgaatcta ttatatgctt tggcatctct ggttcaactt ctatttcttt tccttttatc
     1201 cactgccatc ttggagcatc tagcgcctct tgtggattta atccaaaatc aatcatattt
     1261 gttaacactt gaatatgtcc ttgtggttgc ataaaagctc ccattacacc aaatggacct
     1321 attgctttat cttctttacc taaaaatcca ggtattatag tatgataagg ttttttaaat
     1381 ggttttacta cattgtggtg tttagggtct aaattaaaat tatttcctct actatgtaaa
     1441 gcaatcccag ttcttggtat cacaattcct gaaccaaaat ttatgtaatt gctctgtata
     1501 tatgaaacca tatttccatc cttatctgca gtacaaaggt atactgtacc accacaatat
     1561 ggctctcctg cagttggata caaagcctct ttattgtcaa tcaataaact tctttttttg
     1621 gcatactctt ggcttagtaa ttcctgaatc tttaccttca tatgctctat atcagttaca
     1681 tatgttttag aatctgcaaa agctaatttt aaactctcta taattttatg tatgtcttct
     1741 acattttcta tatttccttc taattccaat tcttttaata tgttaagtgc cattaaaact
     1801 gttattccat gaccatttgg tgggatttca aatattttat atcccttata ttctgtactt
     1861 ataggttcta cccatgttgg ataaaaacac tctaagtcac tctttcttat agccccatta
     1921 aattttttag aatattcatc tattttatca gcaagtcttc ctctataaaa gctttcagcc
     1981 tgagtatttg ctatctcttc taatgtactt gcctgttctt cacatataaa tatatctcca
     2041 gcctctggag ctttaccatc tttagaaaat gtatcaaacc atggtttgaa ctcttctcca
     2101 ataagttctt tttcatagag ttcatatgat tttttccata ccttagctac attaggagat
     2161 actacgtatc cctctcttgc atagtttatc gctggagata agcattccat taagctaagt
     2221 ttaccatatt ttttatttaa ttctgaccaa gctgctggta ttccaggtac agttactgct
     2281 ccaaacccat attttggcat ttctttcata ttattataat tttttaattc catattttct
     2341 ggagcaaagc cactagaatt taaaccatac aatcgttttt cttcctctat ccaaacaagg
     2401 gcatatgcat ctcctccaat cccattgctt gtaggttcta ctactgctaa tgtggcggct
     2461 gttgcaactg ctgcatcaac agcatttcca ccctttttta gtatttcaag tccagcttga
     2521 gatgctaatg gtgttgatgt tgcaactgct ccatttttag catacacgac atttcttatt
     2581 gatgagtatt tatgttggta ggcattaaac ttcataaatt tcagctcctc gtcatttttt
     2641 atttgatatt ctgcttagat ttattatata acatcaagta atgtaactga tacaaaatga
     2701 tataaaatat agactttttc ctaactttaa ttttattttt aaataaatat cttaaactca
     2761 attctagtat tttctatctg tacttaaata agttatttta ttcaaaacat atatctaatt
     2821 cttttaactt aaaaataatt tattctttta tcatgtttag agcatatttt aaagttaaaa
     2881 aataatatat aaaatatcct ataaaataaa tgactagagt tatgataatt ggtacaagta
     2941 atgaccttgg tataaacttt tgtgctatag acatagcaaa taaattatga actgtaccta
     3001 caactaatgg cattaaaaat ataactttta actgttttga tattattttc tcaatatctt
     3061 tattatttgc tccaagtttt attaacataa tatatctttc cttatcatca tatatatttg
     3121 ataattgttt aaataagatt atactacctg tacataaaag aaatactaca gacaaaaata
     3181 tgcctataaa cagcattaga ccacttaatt ttatcaagtt attataactt tcaatacttg
     3241 atgtaaaatt aaaaggataa gtaaatttca tattttcact tactatattt tttaatttta
     3301 aagtcaaatc cagttctctt ttttcatttt ttatatcaat taatcttatt aatctagaat
     3361 ttccatgtaa ttttaactga ttaaatacgt catcttttac tacaattaaa tcaaacgtag
     3421 cttgtgaatt tatttctggc tccaagatgc ttttttgtac tttaaacaga tttatatcat
     3481 actcatttgt atctttaata tgcaagttta ttttttcaat ctcaaagtct ttaaaaaata
     3541 tttttttgta tgaatcagaa acatagtaca cagattcctt tgaagttaat tcttcataat
     3601 tgttattctg atattgtctt aattttttaa aatcactttc tctaataata tctacagacg
     3661 ttctatcttc tatataacta tttccatttt tataatacat ttcaatattt atatctttgt
     3721 ctataagttc tattgtttta tcaaagatta tcttattatc ttttttatat ttatataata
     3781 atttgtcaat ttcgttattt acataagaat ttcctaaatt tatggtataa ctgtatttat
     3841 aattttcact tatgttccta tctatatcgt aataaagtga tatggtaaac cctagcatag
     3901 ttacacttgt agctattaat attgctatta cagccaatat actactattg ctctttaatt
     3961 taaatccaag ttcagaaaat gcaattaaat tctgtccctt ataataaaaa ctttttctct
     4021 tcttaacagc atcaataatc atatacatcg ttgatgaaaa aaataaaaaa gttccaggta
     4081 ttattgtaac taatataaaa actgaaagca taatattttc agcaataaaa taactaattg
     4141 acattaaata agaaatagct attaataaaa caccaagtac acattttaac tttataaatt
     4201 ttttaaactt atacttttct ggatatttat taaataactc tataattctc ttatttctga
     4261 taactatagt atttcttatt ccaataacta tgaatattat taaaaatacc gctagtgtct
     4321 gaactaaggc ctttatactt aagttcatat ttactactat catttcattc attaatttta
     4381 caagcatcat agtcataagc tttgaaaata acagtcctaa aaatatacct attagtattg
     4441 caactatacc catcaaaaaa gtttcatata aaaatatttt tcctatctga tttttcctca
     4501 ttccaagtaa actataagtt cctacttctc gcctacgtct attgaaaaaa aatgagtttg
     4561 aataatatat aaataaaaat acaaatacca ctattacaat tgaacttgta ttaatactca
     4621 tcctagtttt ctctccaagt gaactcaacg cttcattata ctctatagat ttaaaactaa
     4681 aaaatacaaa tacactaaat acaattgaaa taaagtacat taaataatta aaaaaactat
     4741 tctttacatt ttgcattgca attctaaata aagtcatttt tattaactcc tccaattaag
     4801 gaaactgtat ttactatttt attatagaac tctctgttac tttctccctt tacaatttct
     4861 gtgaatatta ttccatcctt tataaagagt attctattgc aaaaactcgc acttatagca
     4921 tcatgagtta ccatcaatat tgttttattg ctgtctttgt ttaattttaa tagacaattc
     4981 aacaaatccc tagcagattt agaatctaat gctcctgtag gctcatctgc aagtactaat
     5041 gatggttttg ttgctatagc tctacataca gctcctcttt gcttttgtcc accagaaact
     5101 tcgccagggt atttatctaa tatagattct atatttaatt cttttgaaat ctcttttatt
     5161 ctattttcta ttactgatac tctttcattt tttaaagaaa gaggcaacat tatattttct
     5221 tttattgtaa gtgtatccaa aagattagaa tcttgaaata caaagcctag attatcacgt
     5281 ctaaaatctg ccaattcatt ttgcttcatt ttaatagtat tctttccatt tatataaata
     5341 tctcctgatg aaggtaaatc tattgtagaa ataatattta aaagtgttga tttaccagca
     5401 ccggaagacc ccataatacc aacaaattct cctttataaa ccttgaaagt tattcctctt
     5461 agagcttcat gttttgaacc ttttcttcca tactcctttt ttatattttc aacagataat
     5521 atttcttcca tagttaataa tcacctttcg tcacaattag ttatttatca attctcacaa
     5581 atatattata tatattaaaa tggcttttaa acatttaatt atattaattt aaggttacat
     5641 tttcgtaagt attaattata tttcttcaaa tttattgcaa ttatgtttaa aatataattt
     5701 atcaagtaaa atatagttat attaatataa ttattttagg taataaggta gaaatatttt
     5761 aatttatcaa gtagaatata gttatgttaa tataactatt ttgaattagg aggtaagaat
     5821 gtttaaaata atggtagttg aagatgacgt ctcattgaaa aatatcatag caaagtgtct
     5881 tactaaatgg ggacatgatg ttcatcaaat agaaaatctt gaaaatataa tagaagagtt
     5941 caaaaactat aacccagaat tagttttatt ggacataaac cttccatttt atgatggttt
     6001 tcattggtgt aatgaaataa gaaaaatttc aaaagttcct ataattttta tttcttcaag
     6061 aaattctaat atggatgtaa taatgggtgt aaatttaggt gcagatgatt atatacaaaa
     6121 acctttttct gtagatgtct tagtagctaa agttaatgcc cttttaagaa gaacttataa
     6181 ttttgttgat aataactcaa atcagattat tcacaatggt gttactttag atttatcaac
     6241 tgcaacaata aactatgaag ataatactat tgagctaaca aaaaatgaaa tcaaaattct
     6301 ccatgagctt atgaaatata aggggcagat agtaagtagg aataaactca tgaaaaaatt
     6361 atgggataat gattggtttg tagatgataa tacattaact gtcaatgtaa atagaatacg
     6421 ctcaaaatta aatgaaattg gtttagaaga ttttatagaa acaaaaagag gattgggtta
     6481 tataatcagt taaaacaatt ctaagtaggt gaatttatga gctttaaaga ttttctcaag
     6541 gataaactag gttttttagt atacaatttt actttcttaa tttttacagt atcagttatt
     6601 atattttctc ctgtagatgt ttttctgact gatactattt tatatatatt agttgtaaat
     6661 gttgtgtttt tatttttata tctatttatt agctacatca agaagaacaa atttttagat
     6721 aagataaaaa atgatacatt caaaattaat atgaatgata tagaattagc tagatgtaaa
     6781 aatgaagaaa aaatatactt gaacattatt aaaaatcatc aatatcaatg taataacata
     6841 ataaataata atatcgagaa atttgaagaa aacaaagaga ttttaaatat gtgggtacat
     6901 gatgtaaaga tacctatagc tataataaaa ctcattcttg aacaaaatga aaatttaatt
     6961 gatgaaaaaa tttcagatga catagatagt gaaatattta gaattgaaaa ctccgttgaa
     7021 aaaattttat atttatcaag actcgaagat tttcataagg attttttaat tcaagaggta
     7081 aatattgaaa aaatcattag aaatattata aaaaaatact caaaatattt tatttcaaaa
     7141 aaaattattt tagacttaaa taatttgaat tttaaggttt tatctgatga aaaatggcta
     7201 tacttcatat tcgagcaatt attgagtaat tccctaaaat actcctcatt aggagataat
     7261 atatctatct attgcaaaag aaccaaaaat tatttacaat taagagtgga agatactggt
     7321 tgtggtataa aaaaggaaga tttaaatcga atattcaata agggatttac aggtaataat
     7381 ggaagaaaca attctaaatc tacaggtctt gggctttatt tagtaaaaga actttgtgat
     7441 aagcttggtc ataaaattga tgtagattca gagtacaacc aatatacaaa atttactatt
     7501 acattcaaat gtgatttata gtatcttatt attgatagtt tattttcatt aacttatatt
     7561 caacttctta tagtaattct atatgtacat cccagtatat aattaagtag tttttataaa
     7621 aaggaggaag acatgtcttt tctaaaaaaa tttaaaaata aaagatttag aaataacagt
     7681 tttaaaagta acaactttag aaatagcagt tttggaagta gtggcttcaa gtctaaaaat
     7741 aaaggtttta aaaacaatcc ctttaaaaat aagagattca aaatatatgc aggaatagca
     7801 gtaatcatac ttttaattct aggagttctt gcttatgttg attcgaaaaa cactgaatta
     7861 caagctaatg aaaattttat cgaaacatat actattcctg aaaatgaaaa aatatttata
     7921 aatgggatgg ttgttcctaa gcaaactaag gacttcaata tatctggaga ttatgagtta
     7981 agtgatgtta atgttacaaa tggtcaaaaa gtaaatcaag gagaccttct ttttactgct
     8041 aaaaatccaa ctataatatc agaaatagat agtttaaaat cacaacttag tcaatacaaa
     8101 aaacaaaaaa tatcactttc tgatattgct gaaaataaag atgctattgc ttctataaat
     8161 gcacagataa ctgctttaaa ctctcagata gcttctctgg ataaaaaagc ttatgacaga
     8221 gttactgctc cttttgatgg cacagtatac ttgaatgacc aaactggaaa tccagaccaa
     8281 ccagtttcat ttatgactat tcaaggtcta gagttttata tgaaaggaca agcaagtgaa
     8341 caagatttac ctaaaatgaa gattgaccaa atggtcaata tactagtttt ttcaactgac
     8401 caaaagctta ctggtagaat aagttttata tctgataaac catctactcc aaatactgaa
     8461 atgggagctc agcagaatac actatcttat tatgatataa acatagcatt tgataatcaa
     8521 gaaggattag taaatggatt ccatcttcaa gcatcacttg aagtagctaa ttcaagcttt
     8581 aaaataccag catcttgtgt acttaaagac aacaaacact catatgtatt taaggattta
     8641 gatggtatat tgaaaaagca aattgtagat gtagcaagtc aaaatgatga ttttgctgta
     8701 gttagaggcg gtcttgaaca aggcgatata ataataaaac accctactaa agaaatgaaa
     8761 gaaggagacc cagttcaagg cgatggcgta actgctggtt ctaataatgg agatactact
     8821 ccaaacaaaa aagtagaaga agttactatg gatgtgaatt aatttacata tgttaattaa
     8881 attagaaaat attcaaaaat attacaaagt agggaaagat gaattacatg ttttaaaatc
     8941 cttaaattta gaaattgaat ctggagaatt tgtcatgata atgggtaagt ctggtagtgg
     9001 taaaactact ttacttaata tcttaggttt tctagatgta tttgatgaag gaagatatat
     9061 atttgatgga acagatgtaa ctaatttgag tgaaaatgaa cgttctgttt ttagaaatat
     9121 taatattgga tttgttttcc aacaatttaa tttaatagaa actctaaatg tatatcaaaa
     9181 tgtagagttg cctctaattt acaataaagc actaaaaaaa tctaatagag aagaaattgt
     9241 taaagataaa ttaagttctg ttgggctttt ggacaagctt aagcagaaac cacttcaatt
     9301 atctggtggt caacaacagc gtgtagccat tgcacgttgc ctagcaaatg accctcagat
     9361 aatctttgct gatgaaccga ctggggcatt agatagtgaa actagcagag aaattatgga
     9421 gcttttaaca aggctcaaca aacaaggtaa gactataata atggttacac atgatcaaga
     9481 tttaactaag tatgcaacta aggttatacg acttaaagat ggtgtgttta cttcggaggt
     9541 gtaatcatga gtttattaaa aaattctttg gccaatttaa aaggtcacaa gcttcgtgta
     9601 tttgtagctt tactctggat tataatagga ataacttcag taatacttgt tagttctata
     9661 ggtaatggat ttcaaaaaga aattaagaaa tctgtaaaca atgtaaaccc taataaaact
     9721 actatttcgt ttgaatcagc cgataacaca ggtcttactg atgatatgag tattttttta
     9781 aaacccttta atgcaaaaga tttagaagaa ttatcatttg ttgaaggagt agaaagaata
     9841 gctccttcaa gagatgggtt taaccttgat tcagtttact cttcacaagc atcttttgat
     9901 aaaaaaacta cgtatgttga tgttggacca gtaaaaaaag attctaaaat aaatttaatt
     9961 tgtggtagag atttttcttt agatgatgaa aaaagaaaag taattttact tacattacaa
    10021 agtacaagtg aaatttttga aaatccagaa gatgccttag gtcatggtat caacataaat
    10081 ggaactatat ttgaaataat aggggtatta gatgattctc agcaaaacca agctggagga
    10141 ttctttggag gttaccaaga tatgcaattt actacttccc tagtacctaa aaaggctttt
    10201 gacactttaa tgagtcaaaa ttcttattca aatgaaattt accagttaga cttagtttct
    10261 tctaaaggct ataatgtaaa tgaagttgca aacaatgtta tagctaaatt atatgagatg
    10321 caccctggta tcaatggttc ttatactaca ccagacccaa ctgaacagac agcttatctt
    10381 gaaagcatta attccaatgt aaacaaatat gtatctataa taactgtagt tgcaatgttt
    10441 gttggtggta ttggtgtaat gaatataatg tatgtatctg ttatggaaag acaaagagaa
    10501 attggtataa gacgtgctat tggagcaaaa cctaggtcta tattatttca atttttagtt
    10561 gaagctgttt ttataacagt atgtggtgga attttaggaa ctatagttgg atttactgct
    10621 actaactatg tttctaaata tataggtttt gaagctatac ctagcttaaa tagcttattt
    10681 tatgcaattg tagctactat attgactgga gttgtatttg gtctgattcc agcctttaaa
    10741 gcctctaaac tagaccctat aaaggcaatt tataagtaga aattgtataa agattttcga
    10801 tttttagatt agtttatata aaacatactt aaatatataa cgcactatta tatatgatag
    10861 attaggaact taatctttcc aaatataata gtgcgttctt tcataaaaag gattctcttc
    10921 cttactatat ctaaattacc aagatgttat gttctaattg ttcaaactaa tacttccaat
    10981 tttgattaat caatctcatt ctcctaatat acaatctttt actttcaatc attgtacact
    11041 ccgccttaaa aaaataatct taaattaggc gttatataca atctccccat tacatattgt
    11101 ataattaacc tttccatata aagatttacc tttaaatggt gaattatcag ctttagaaac
    11161 aaagctttca actgtccatt cttcttctgg attgaatata acaatgtctg ctacagcccc
    11221 ttcttctata aatccaatat ttaaattgta caactttgca ggatttatag acattttttc
    11281 tattaactgc atcatactaa gatgattttt atgcaccaaa tttgttattc caagagctaa
    11341 agaagtttca agcccaataa ttccacttgg agcttttata aactctctat ctttttcttc
    11401 tttactatga ggtgcatggt ccgttgctat tatttcaata gtaccatcat ttaatccctc
    11461 tataatctta tctctgtccc atttacttct tagaggtgga ttcatttttg catttgttcc
    11521 aaactccaat acatcttcct cagtcaatgt aaagtgatgt ggtgaagctt ctgcaactac
    11581 atttgcacct aagtattttg caaatctaac catatcaaca gatacacctg aacttatatg
    11641 ttgtatatca actttagcac cagtctttag tgccaacata caatctctgg ctaccataac
    11701 atcttctgct acacttgatg caccttttaa tcctagttct gaagctacct ttcctgcatt
    11761 tacacctgga tttccaacta aagaagggtc ttcttcatga aaactaagtg gtacatccaa
    11821 ctcttttgcc ttaatcattg cctccataat tagattgcta tccattaaag gtaatccatc
    11881 atcagtaaac cctggtaccc cagctttttt aagagcttcc atatcaacta actctttacc
    11941 ttcaaaacct ttagttatag ctgctgcttg taaaacattt atacatgcat tttttgactt
    12001 ttctttaaca taattaaaag tatcttcatt atctacaata ggattagtat ttgccataca
    12061 tataacagtt gtaaatccac ctctagcagc agatttagct ccactttcta tatcttcttt
    12121 atatgtaaac ccagggtctc taaagtgtac atgtacatcc actaaacctg gagcaactac
    12181 attaccacta gcatctatta ttttttcata ttcatcactt tcatcaaact tgcctatttt
    12241 ataaatttta ttttctttta taattaagtc aatattctca tctctttgac ttttagggtc
    12301 tattactcta ccatttttaa ttaaaatcat attatttctc ctttatattt ttaatttata
    12361 tagactcata taatattata cattatttta catacttaaa tacaaaatta cgtttgacat
    12421 caatagcctt tttaggacaa agttcatggc aacagtaaca tcttatacat ttacttaagt
    12481 ctataatagc acccttagtc cctatctcta tagtggatgc aggacaagct tccttgcatt
    12541 ttccacattt tatgcaatct tgaaatctta ccacaggctt tggtgttata agctttgaaa
    12601 taggttcatt taaaaatttt ggtagcttta cagaaaacaa tcttaaatcc ttacttacat
    12661 ttggaatctt gtaatttttt ataattaaat cttttatatc ttcacctaaa atttcaatat
    12721 ctgaaaaatc ttcttgtata tatcctctct taatagcacc tctaattgta gcaactctat
    12781 ttggaagtaa acttataatc ttacatgcaa caacatctat agcatatgga ttttgtgatg
    12841 ctaataaaac tcctatttct cgagtagtac cagctgaagg tccatcacct tccataccta
    12901 ctataccatc cataattgtc aatgttggtt ttatataatc acaaatatct aaaagtacat
    12961 tttcgcaaaa caactcttct gtaggaaatc tataatgtat ctctgctttt ttcaatccag
    13021 ctatgcatcc atataagttt tttgtaccac cagtaaaagt tgccattata tgagtcttta
    13081 atttacaaag atttattata taatctgaat ctactattgg tttaataata tccatatatt
    13141 ttagaacatg tgctttttca ctatgtatct taacatctga aacatcataa tttaacttaa
    13201 tgcccagttc atttgctact tcttcaattc cagatgcctt ataaatgctc ttaagagaac
    13261 tttgattata tggaccacca gggctatctg ctattgtaat atcacaattt aactctaaaa
    13321 gtttttcaca tacaactttt acaaccatag gatgtgtagt tgtagcttct tcaggctttt
    13381 ttttcatcaa taaatttggt tttataagta ctttgcttcc ttttttaaca aacttatcta
    13441 taccaccaat atcctctaaa tttttttcaa tagataactt tacttcttca taattgtaag
    13501 ttttacaact cctaacagat accgacttca tattatctcc atccttctat cacaacagtt
    13561 tatttgtata atttaaaaaa ggagaaacca aagtttctcc ttttaatcta tgttttaatt
    13621 attattttcc aaaatgtctg tttaataatc ctaagaatgc tttaccatgt ctagcttcgt
    13681 ctttacacat ttcatgtact gtatcatgta tagcatctaa tcctaattct ttagctctct
    13741 tagctaattt taattttcca tcagttgcac catactcagc atcaactcta actcttaagt
    13801 tttcttttgt atctgcaacc acaacttctc caagaagttc tgcaaattta gcagcatgtt
    13861 cagcctcttc aaaagctatt ctcttatacg cttcagctac ttctggataa ccttctctat
    13921 cagcttgtct actcattgct aaatacattc ctacttctgt acactcacca gtaaagttag
    13981 ctcttaatcc ttcgattatt tctgcatcta caccttgagc tactcctatt ctatgttcat
    14041 cagcccaaac catttcgcct ttcatttctt caaatttatc agctccaact ttacacactg
    14101 gacattgtgc tggtgcagca tctccttcat gtatatatcc acatactgta caaacaaatt
    14161 ttttcataat taatttcctc ccaattttat ataattaaat ttttattttt aagtttaata
    14221 acttgctaca agaatatata taccccagac tttatagctt aaacactaaa aataaaattt
    14281 atttcctaat taattttatc actttgaaac accagtatca accaagtttt gatgtcaaat
    14341 ttctacataa tacataattt atttgattaa ttaggaaata gttgtcatat taaatacttt
    14401 ctaatgaaga ctcctctgaa tctttaagtt tcactttttt gctctttaaa aaagataatt
    14461 tagtttctgc agttatgatt gcaacaaata tcaagatact accaataacc aattttgctg
    14521 taataagttc ctttagaatt attatagaaa atattgtacc aaatacagcc tctgtggata
    14581 atattatagc tgttctagtt ccatcaactc ttttttgaca tatagtctga aataaaaatc
    14641 caattgtagt actaaataca gctagataca tagttcccat gtaaccagat aattcagctt
    14701 ccatctttaa ttgacctgca aatgtttgaa ctacaacaga cattaaaaac gctactgtaa
    14761 attgaatagc tgtcaatgcc attgggttgt tatcttttgc aaactcactt gtaaagaata
    14821 tatgaaatgc aaatccgaat gaacatataa gtgttagaaa atctccaaaa ttaatagaga
    14881 aatcagcttc taaagaaagt atcccaattc ctataagagc taccaaacta cttattattc
    14941 ctattttatc aagttttctt ctataaagta taaatcctat gaacggaact attacaacat
    15001 tagctgctgt tataaacgca tttttagatg gtgtagtgta cataagtcca atagtttgta
    15061 ctgtgaatgc tagaaataaa aatattccta aaatccctcc tgcttttaat aattttttac
    15121 ccatattggc acgaatctgt ttaaaaaaca gtaaattcat aattatagct gccaagaaaa
    15181 atcttaaagt tatgatttgt agaggtgtaa gcccaccgtc taatgcaaat tgggttgcta
    15241 caaagccact tccccatata attgctataa atattagtcc tatttctcca aagtattttt
    15301 tcaataaagc cccctccttt ttattttttt gcaaaattat aaacccccta aacagcaaga
    15361 gagtttttct ataagcatta ctataaatta atatagtaaa ttttttcagt attttaaatc
    15421 taaatttaca tacattttga gctcaaattt atatccacta tttaacagta atatattaat
    15481 cattttttgt aaacaccttt ttaataaatt tttatatttt ttatattttt ttacctatac
    15541 taaatttttt tatagatatt tttaatatat atgatacttt aatattatgc aaataatgtc
    15601 atataagtta cactttagtt atcttagtta caactttgtc ggtttacttt ttctactttc
    15661 ttcgatataa ttataatata tttttgatac aattcgacaa aagggggaat gctgtaatta
    15721 tggtaaaaaa aagaaaaaag aaaactgtta cacttactat acttgttgta atagttattg
    15781 gtattttttc tatggcatgg gcggacatgt ctagaaaaaa agaaatgaaa gaaactcaag
    15841 aaagacttaa agctgaaaga ttagaaaaag aaagaattga aaaagaaaaa aatgaaccta
    15901 ttattggtgc aaaaaaagaa gctgtaaaag aatatggata tgacgcaaaa atagtttggg
    15961 acaaacttaa caaatacgac tattcaaaca atggagaaaa gatagtcttt cttacatttg
    16021 atgatgggcc atctactact aatactccac aagttctaga tatcttgaaa agacatggtg
    16081 ttagaggaac tttctttatt aagggtgatt ctcttgaaag aaaaggagct aatgaaattt
    16141 taaaaagaac atttgacgaa ggaaatgcaa tagcccatca ctcttatact catgactata
    16201 aaaaactata tcctaacaga agcctaaatc tcgatacatt cgtaaatgaa cttaacaaaa
    16261 ctgatgaagc tatgaaaaaa gtcttaggaa agaatttttc ctcaaatgta gttagatgcc
    16321 caggcgggta tatgtcatgg aaaaatatgg aacctctagg aaactactta aaagagaaaa
    16381 acatggcatc aattgattgg aacgctttaa atgctgatgc agaaggtaaa aagaaaaatg
    16441 cacaagaatt atttgaacat gcaaagaaat cttctgaagg caaagaaatg gttgttcttt
    16501 taatgcatga tacttatgga aaacaagaaa cagtaaatgc attggaccaa attataacat
    16561 attttaaaga caatggttat caatttaaaa ttttaatata ataaaaaagc aagtttattg
    16621 aatgtacatt ttatactcaa tagacttgct tttactttac tctattttac tagctcgatt
    16681 tttacaaatt ttttctttcc tatttgaaca actaattcac tctcttttac aatagtgctt
    16741 aaatcctcta ctttttcacc atttaccttt actccacctt gagaagctaa tcttctaact
    16801 tcactcttac tcttaacaat ctcattagaa actagtactt cgattaagtc aaatccatct
    16861 tccttaactt gaattgtttg tatatcttca ggtatttgac ctttttggaa tacagattta
    16921 aatctttctt cagcttcttt agcagattct tctccatggt ataatgttac tatttctctt
    16981 gctaagttca tctttatatc tcttggattt actgaaccct ctttcaactg agattctatt
    17041 ttattaactt catcaggatg aacatctgtc actaaattgt agtatttaat tataagttca
    17101 tcaggaattt ccatagattt ttggtacatt attcccgctt cttcatctat tccaatatag
    17161 ttacctaaac tcttactcat tttttcttta ccatctaatc cttctattaa aggcatcatt
    17221 attactattt gagactccat accaaattct ctttgtaatg atcttcccat tagtaaattg
    17281 aatctttggt cagttccacc aagttctata tcagcttcta atgctataga gtcatatgct
    17341 tgcattaatg ggtagaagaa ctcatgtact gatattggca tttgtccttc atatctcttt
    17401 ttaaaatctt ctctttctaa cattctcgca acagtaatag ttgctgctaa ttttattaca
    17461 tcttcaaaat ttaacttagc taaccactcg ctattaaatc ttacaatagt tttttcttta
    17521 tctaaaactt tgaaaatttg ttcttcataa gtttttgcat ttgcaagaac ttgctctgtt
    17581 gttaaagctt ttctagcttt agattttcca gttgggtctc caatttttcc tgtaaaatct
    17641 ccaattatta ttactatttg atgacctaaa tcttgtaatt gtttcatctt tctaagaact
    17701 actgtatgcc ctaaatgtat atctggagca cttgggtcta gacccaattt tactatcata
    17761 ggctttcctt ctttttctga tttaataagc ttttccctta actctttctc atctattaaa
    17821 tcatctacac ctttcataat tattcgcatt tgctcgtcta tcgatttcat ttttcattcc
    17881 tcctaaaata tttatataat ctataatttt tatttttata ataaaaaaag ctttcatcct
    17941 cttaaaagga cgaaagcatt gcttacgtgt taccacctta gttcatcaat atctcacgat
    18001 attaatctct tagagtacaa ttacatatac tcgtgtacta taacgtgtac taatccgata
    18061 tctcttaatg gttgttatct ttcagagata gtgctctgag atgtattcaa actaaatcaa
    18121 tttattccat ttcaccaaac tggaactctc tgttaaataa aatatagccc tacttcttct
    18181 cttcatagca tttatatatt cagattattt catcaattac aagaaatttt tcttaactta
    18241 ctcataatat attattattt ttatattttg tcaacctttt ttattattat atacatgttt
    18301 ttttacatta tactactttt ttatattttt taccaatacc caaaatttat ttttcaatca
    18361 aaagatttat ttgtttattg actatattac tctgactcaa tcttatattt attttcttat
    18421 ttaaaaatgc gtttataatt attaaactaa taactgttac atcaaatata aaaaacagca
    18481 ataaaattat tatcaaaaat aaaattctag catataataa ttttctaaac ttagctatta
    18541 taaagttttg tagtgacata catgagtctc taatgataaa atagacactt atataggtta
    18601 ttgatataaa gattgttttt aaataaacag aatcaacttt aacattcaac agttttagaa
    18661 cttcaaaaat ggctagataa gaaacacata tgtaaaaagc tttttttgag caacataaaa
    18721 attggtttaa atttctaaag ttcttaaata ttaaaaattt ttttaaagaa ttagaatcta
    18781 aaatatgcaa tttattccct aaaattaaac ttaataaata tatgcaaaac accataccaa
    18841 aaggaaacaa tgctatcatt aaataataaa taaaatttgt atacccatta aaataaatcc
    18901 atttgctata tccttgaaat tctttcataa aactataacc tattaatgtt gaagcatata
    18961 tatcaatgaa aaccattaca ataaccccaa atactgaacc atatactaaa tcatccataa
    19021 ccattgtagg cttatttatt agatagccac acattatacc aagtaataca ctctgacttg
    19081 cccaaatagc agttcctaga tttccaagta ccagagaaat tattaaaagc gaagtaattc
    19141 cagataaaat ggtatacttt aaatcacatt tcaagcagat tatacaaaaa aatactggca
    19201 ctataaaatc taagtaaata gcatatccaa atccagtact cacagctata attgttacta
    19261 ctatgaataa agatgacaat aaagctgctt cagttaattt tttagtagta ttcaaaacac
    19321 tgcctccata aatcataaat tttctaacta aagttttgtt tttaatatag tatatcactt
    19381 tttaaaaata tttttaataa atataaattt taataaaggt atttatgtta aaaatggtat
    19441 aatgtatctt aatattcagt tttttaagat gctaggaggt aattactatg tttaaaat
//
LOCUS       DAGSWI010000072        19461 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.072, whole genome shotgun sequence.
ACCESSION   DAGSWI010000072 DAGSWI010000000
VERSION     DAGSWI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19461
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.072"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            236..757
                     /locus_tag="MJF80_002332"
     CDS             236..757
                     /locus_tag="MJF80_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0280989.1"
                     /translation="MDIAKLILNPARLRILQYIRLHSSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            791..1567
                     /locus_tag="MJF80_002333"
     CDS             791..1567
                     /locus_tag="MJF80_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBZ0280990.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKM
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1909..2862
                     /locus_tag="MJF80_002334"
     CDS             1909..2862
                     /locus_tag="MJF80_002334"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBZ0280991.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2880..3899
                     /locus_tag="MJF80_002335"
     CDS             2880..3899
                     /locus_tag="MJF80_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBZ0280992.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEENTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3930..4181
                     /locus_tag="MJF80_002336"
     CDS             3930..4181
                     /locus_tag="MJF80_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0280993.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4463..6268
                     /gene="lepA"
                     /locus_tag="MJF80_002337"
     CDS             4463..6268
                     /gene="lepA"
                     /locus_tag="MJF80_002337"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBZ0280994.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6417..7754
                     /locus_tag="MJF80_002338"
     CDS             6417..7754
                     /locus_tag="MJF80_002338"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBZ0280995.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIHCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEAIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVGLFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            7987..9300
                     /locus_tag="MJF80_002339"
     CDS             7987..9300
                     /locus_tag="MJF80_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBZ0280996.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9472..10647
                     /locus_tag="MJF80_002340"
     CDS             9472..10647
                     /locus_tag="MJF80_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBZ0280997.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINVDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10750..11769
                     /gene="hrcA"
                     /locus_tag="MJF80_002341"
     CDS             10750..11769
                     /gene="hrcA"
                     /locus_tag="MJF80_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBZ0280998.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11799..12419
                     /gene="grpE"
                     /locus_tag="MJF80_002342"
     CDS             11799..12419
                     /gene="grpE"
                     /locus_tag="MJF80_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBZ0280999.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12511..14358
                     /gene="dnaK"
                     /locus_tag="MJF80_002343"
     CDS             12511..14358
                     /gene="dnaK"
                     /locus_tag="MJF80_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBZ0281000.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14569..15723
                     /gene="dnaJ"
                     /locus_tag="MJF80_002344"
     CDS             14569..15723
                     /gene="dnaJ"
                     /locus_tag="MJF80_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBZ0281001.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16689..17624
                     /locus_tag="MJF80_002345"
     CDS             16689..17624
                     /locus_tag="MJF80_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBZ0281002.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EGLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17660..19033
                     /locus_tag="MJF80_002346"
     CDS             17660..19033
                     /locus_tag="MJF80_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0281003.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19221..>19461)
                     /locus_tag="MJF80_002347"
     CDS             complement(19221..>19461)
                     /locus_tag="MJF80_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0281004.1"
                     /translation="LIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTLVY
                     RLEKINEMLGFDLKNFENAMIFYLSVKSYFLYKKI"
BASE COUNT         7714 a         2144 c         3533 g         6070 t
ORIGIN      
        1 taattaagat aatttttcca ataatcaaga taattttttt cattagtatg tatgatagat
       61 gcatcaaaca atataactaa tatgttggaa ggttatatgt aattaatctc agtgtttata
      121 tagaatagca tttgtagcat ttattatagt tgtaattact atttatatca tagccttgaa
      181 tagtctaaaa aaatagacta taatattttt atagactaaa gaaagggctg ataagatgga
      241 catagcaaaa ctaattttaa atcctgcaag gcttcgtatt ttacaatata ttcgacttca
      301 tagcagtgtt cgcacatctg atattgtaaa gtatttaaat gatattcctc gtgcaacagt
      361 ttatcaccat gtaaaaattt tagaagagaa caatatgatt gaagtaatta aagaaaatcg
      421 tgtgcgtggt actattgaaa aggtttatgc actaaaggaa tatacaacat ctatggaagg
      481 agagaccttc gttgcacttt ctacagcatt ttatgtaggt ctgatgcagg aaatgaatga
      541 atactttagc agaaaagagc aagaccataa aaaggataat gtatttttct cttcagcatt
      601 actatatatg agtgataatg aatatgaaaa tcttcttaaa agtatagctg atttattgaa
      661 accatatata gaacaaaaac ctaaatcaaa tctaaaactt agaaaacttt caattatatc
      721 ttctccacct gtagctgata atgataaaac aaaataacat gcaacccatt tttataaaag
      781 gaggttatat atgaaatttc atgagtttgg aaataaaaac agaccacata ttatgttgat
      841 tcatggggga gggaatgcat ggtggaacta tttgcgacaa gcagaagttt tgtctaacaa
      901 atatcatgtg atacttccta cattggatgg tcatggagaa gaatatgaaa caacatacat
      961 ttctacagag gatacagcag ataagttgat atcatatata gatgaaaatt gcagtggaca
     1021 tcttttcgct ttgtgtgggg tatcacttgg tggtcaaatt gtcatggaac ttttatctcg
     1081 aaaacctaat attactaaaa aagcaattat tgatggtagt atttgctacc caagacctct
     1141 tatggcaagg ttttgtattg ctggtattcg attttttgga ggacttttgt ttagtgagaa
     1201 ggcttgtcgc tttcaaattg ctatgatgcc aaaattgtta cctgaaaata tgcaatatcc
     1261 agatgaaata aaagcatatt atatgacaga tatgccacat gtaagaaaag aaacattcta
     1321 taatatgtat cgcacttaca tgatgaacta tacacttaaa gagaatgtta aaaaaaccac
     1381 agcacaagta atgtactggt atggagaaaa agaaatgaaa tgtgttaaaa aatctgcaaa
     1441 aatgtttcag tcttatgtgc cctcttgtga gatttatgaa gctaagagct ataatcatgg
     1501 gtatttatca ctttatcttc caaatgaatg gttggaaatt gcagaaccat ttttccaaaa
     1561 ggaataaagg tgttaggtga ttattgtata attattgctg tttttttata tagtaaataa
     1621 attaggtata aatataaaaa attattttag ttaatatatt agtgaaagct taataggaaa
     1681 attataaata gattaaaggt agcgcctata taaatagttg ctaccttttt ctgtttgtaa
     1741 ataaatgata gataagaagg tttactgaca aatcactgac taaacccaat tttcttacat
     1801 tgaaagcctt aattttactt gttttaaata aatttacaat ttcttgttat aaaatactaa
     1861 attttgttaa caattataat tagcatttaa tttatactgg aggtttatat gattagtgta
     1921 agaacagatt tagctttaga agctagtgaa atgtgtgaaa agtctcaaga aggaagtagt
     1981 attcctggtg taaaaataga aactaaagaa ttagaaaatt gcattgtaac taaagttgaa
     2041 gtaatagaca ggcaaggcgc agagataatg aataaagata taggaaagta tataactctg
     2101 gaaagtaatc ttatgaagtt tgatgatgat gagtcaagag aagaaatgat aagttattta
     2161 aaagaggagt tagtagatat atttggccaa gacaaaaata aaaaaaccct ggtaattggt
     2221 cttggaaata gaaatattac ttctgatgcg ttaggaccaa agagtgtatc aaaaacactt
     2281 gtaactagac atctttttaa aaattataat aaagactatg atgatgattt cactgaggta
     2341 tcagctttaa gcccaggagt tatgggtgtt actggaatag aaactagtga aatagtaaaa
     2401 tcattggttg agaaggttaa gccagataga gtagtagcaa tagatgcatt ggcttcaaga
     2461 aaaatggaga gagtaaactc tactatacag atatcaacag caggaatatc acctggtgga
     2521 ggtgttggca atactagaaa atctctaact aaagaaactt taggcgtgga tgttattgca
     2581 ataggcgttc caacggtggt tgatgcagca actcttacaa tagatgtctt agatatggca
     2641 atagacaatt taatagctca atcagaagaa acagaaagtt tttatgaaat gttgaaaaaa
     2701 cttaaagaag aagaaaaata tcatctaata aaagattcgt tagacccata tgacaagaac
     2761 ttaatagtaa caccaaagga tatagatgat acaatcgaaa atctatctat aataataagt
     2821 gaaggcttaa ataggtcttt acatccaggt agattggtat aaaataagga ggagcaatta
     2881 tgtttaaaaa atgcattaaa gtagtaacct tgacttttat attagtatgc attttaccag
     2941 gaaaatcatt agctttgaat caagatgatt ttttaaagtt tttagtaaat tcatcttatc
     3001 cagaagctaa agtcgaagga aatgatacag aaaataaaaa gaataataaa aataaagaaa
     3061 caagtaagga aaataaggaa gaaagtaaag aagaaaacac taaatctaaa gatgcaagta
     3121 aagttgataa taaaaaagag agtgaaaaag aatatataaa actttatgtt ggtaaagaaa
     3181 atgttccaga tattgagtca aaaaattcag acacaactga aactaacaca acatctagtt
     3241 cagattataa ggatgattta agagtaacca aagaaaatcc aagaattttg atatatcata
     3301 ctcatggatg tgagacttat tcaaattcac ctgatggaaa ttatcattca agagataaga
     3361 aaaattcagt aatggaagta ggaagtgcat taactagtgc tttagatagc aaaggatggg
     3421 gtgtagttca tactactaaa taccatgact atccatctta taataactct tatgcaagta
     3481 gcttaaaaac aatacaaagt atattaccta aatataactc agtggatata gcgatagact
     3541 tacatagaga tgctagggat ttaactaatc cagccactaa ggaaaaagac catttaaaat
     3601 atacaactat gataaatgga gagagagtat caaagttctt ctttgttgta ggaggtaaaa
     3661 atacaaatag aaaacaactt agagctttag cagaagatat aactgctttt gctgagaaaa
     3721 agtatcctgg tctagtatct ccaatagtag aaaaagacta tgctagattc aatcagtttg
     3781 cagttaagaa tcatatgtta gtagaaatag ggaataacgc aacaagtgta gaagaatcta
     3841 aggctactac aaagtattta gccgagattt tggatgaata ttttaaacaa aaaaattaga
     3901 gctttaagtt ataattagga gcatgctaaa tgagccgatt agaaaaaaat atagaaagta
     3961 aaagtaaact aagaaaatac aagtatatta tgagaatctt atttttgatt actatgatta
     4021 tatgtacatc agtttgtgta tttatagtgg acagtaatgc aaaaagtatg ctaggaatga
     4081 aacaatatga ttttccaagt attaaatttg atataacaaa atttgatttt gacataccaa
     4141 attttgatat acatgaaatc ataaaaaaat ttaataagta gtaataagtg aataaggttt
     4201 attgattaaa ataagcctta ttttagttat atatttttaa tgtataaaat aaaaattcat
     4261 taactgtatt ttataggatt tactttaagt ttatgatatt atataatatt tagtgttaga
     4321 cttgataatc cacatataat gtggataatt ttgtttagtg ccaattagtt tatattcaaa
     4381 aattttaaat ttggtatata aagagttttt agataatgta aaataataac tataagaata
     4441 atcaagagga gggaatttta aggtggacaa taaacaaagt agaactagaa attttagtat
     4501 aattgcacat atagaccatg gaaagtctac cttagctgat agattaatac aacaaactgg
     4561 gcttgttagt gaaagagaca tgaaaagtca attacttgac aatatggacc ttgaaagaga
     4621 aagaggaatc actataaagc ttcaaaacat aagattaatg tataaagcga aggatggaaa
     4681 tgaatattat ttaaatctta tagatacacc tggacatgta gactttaact atgaggtatc
     4741 aagaagttta gctgcatgtg aaggagcact attagtagtt gatgcagctc aaggtgttga
     4801 ggcacaaact ttagccaatg tttatcttgc tatagaccaa gatttagaaa tattaccaat
     4861 aataaataaa atagatttac caagtgcaag accagaggaa gtaaaaaatg aaatagaaga
     4921 tttaataggg cttgattcaa gtgaagctcc tcttatttca gctaaaactg gtttaaacat
     4981 agaagatgtt ttagaagata ttgtaaaaaa tgttcctcca cctaaaggag ataatgaagc
     5041 tccattaaag gcattaatat tcgattctta ctatgatgct tacaaaggtg ttgtagctta
     5101 tgttagagta tttgaaggaa ctgttaaaaa gggtatgaca ataaaaatga tgaataccaa
     5161 taaaaagttt gaggtaactg aagttggtgt tatggctcca ggtcaaactg aacttagtga
     5221 attatcagca ggtgatgttg gttatattgc tgctagtata aaagatataa gaagttgtcg
     5281 tgttggtgat acaataacag attcaaataa tcctacagaa gaacctttac caggatacaa
     5341 aaaagctact ccaatggtat attgtggtat atatccagga gaaggagaaa agtatgagaa
     5401 tgttagagat gctcttgaaa agcttcaagt aaatgatgct gcacttgaat atgaagctga
     5461 gacttcagca gcactaggat ttggttttag atgtggtttc ttaggtcttt tacatatgga
     5521 gataatgcaa gaaagacttg agagagaatt taaccttgat ataattacta cagctccatc
     5581 tgttatatat agagttacaa aaatggatgg agaggttgta atgatacaaa atcctgcaaa
     5641 cttaccagaa ccatctgaga taaagatgat agaagagcct atagtaaaag gagatataat
     5701 agttcctaaa gactatgtgg gtgttgttat ggagctttgc caggaaagac gtggaaatat
     5761 gttaaatatg gaatatatag atgatagaag agtaatgctt cactatgacc ttcctctcaa
     5821 tgaagttgtt tacgatttct ttgatgcttt aaaatcaaga actagaggtt atggttctct
     5881 tgattatgag gttaaaggtt atgtagcatc tacacttgta aaacttgata tattaatcaa
     5941 taaagagcaa gtggatgcac ttagttttat tgtccatgag acacgtgctt tccctagagg
     6001 aaaggcaatg tgtgagaaat taaaaggaga aattccaaga catcagtttg ctataccaat
     6061 acaagctgct gttggtaata aggttattgc aagagaaaca ataagtgctc taagaaaaga
     6121 tgttcttgct aaatgttatg gtggagatat atctcgtaag aagaaacttc ttgaaaaaca
     6181 aaaagaaggt aagaagcgta tgagacaaat tggttctgtt gaagttcctc aaaaagcatt
     6241 tatgtctgtg cttaaattag atgagtaaat ccttataaat ttagataaag atatttaaaa
     6301 gctagagtga ttttagaatt atttctaaat tagtctagct tttttatggt atattttaag
     6361 taaactcata gagaatataa gtaagaatat taaagaagaa taaaaaggtg atatttatga
     6421 ttaatataga aattcctaaa aaagtagact atataattaa agaattagaa aagaatggtt
     6481 atgaagctta tatagttgga ggatgtgtta gagattgttt attagaaaga gttccaaacg
     6541 attgggatat aacaactagt gctagaccag aagtggtagt agaattattt gaaaaaacaa
     6601 ttccaactgg tatacaacat gggactgtaa cagttatgat tgaacatgaa ccatttgaag
     6661 tgacaactta tagaattgat ggaaattata gtgatggtag acatccagat tcaatagaat
     6721 ttacaaataa catagttaag gacttgtcaa gaagagattt tactatcaat tcaattgcat
     6781 acaattcaaa gacaggatta gtcgacccat ttaatggcta tgaagatata caaaataagt
     6841 atatacattg tgttggaaat ccagtagata gatttgaaga agatgcactt agaatgctta
     6901 gagcagttag attttcagca caactcaatt ttaaaatagc tgaaggtact aagcaaagta
     6961 ttcataaaaa agcagatttg ataaaaaatg tttctattga aagaatccag acagagttta
     7021 ataaaatatt agtatctgat tcatctaaat taaacttatt aaaatcaaca ggtttgttga
     7081 aatttataat tcctgaaata tgtgaattag aagatgttac tcaacacaat ccatatcaca
     7141 tctatgatgt acaaaaacat actttaatag ctacagaagc aattgaagat gaactgtatt
     7201 taaaacttac aatgttgttt catgatttag gtaaaaaagt aactaaaaca actgataaaa
     7261 atggtgttga ccatttttat actcatagta gagaatctgt aaagattgca aagaagattt
     7321 tgaaaagact taagtatgat aattatacta ttaataaagt tttaatatta atacaatatc
     7381 atgattatag aatagaacct aaaaggaaga taattaaaaa gttattaaat aaacttgaag
     7441 atgttggatt atttgaagat ttaataaaag ttaattgggc tgatacactt gcaaaaaatc
     7501 caaagtatgc aaaacaaaag atattaaatc ttattgagtg tgaaaaagaa tttaaacata
     7561 taataaatca aaaggagtgc tttaatctta aagatttagc tattaatgga aaagatttga
     7621 tatctatagg tataaaacca ggtaaagata taggatatat tttaaataag atgcttgaaa
     7681 tagtcataaa taatccagag ttaaatgaaa aagaaatact aaaagaaaaa gcattaaata
     7741 tatatacatt ttagattcaa atataaaaaa tttaattact ttaaaaaaca tagaataaaa
     7801 tacttattta gataaaataa ataattttta gagaatattt tagaagttac gattgtatga
     7861 tttttaaatg cataggcatt tactctagga ctatagacta atataaaaaa aaactttggg
     7921 aatactaaaa ccataaattt ataaaattaa tattttatat actgtaaaac agtgaggagg
     7981 gacagtatgc atactcaact acaaaagact attggtcttt cagcagctct ttctactgtt
     8041 gtaggtatgg ttataggttc gggagttttt tttaagccac aagcaatata cactacaact
     8101 aatggagctc caggtctagg tattatagcg tggcttttag gtggttttat aacaataact
     8161 gctgggctta cagctacaga aatttcagct gctataccaa aaactggtgg tatgatgatt
     8221 tatatcgaag aaatatatgg agaaaagtta ggttttttaa ctggatggat gcaaactgta
     8281 ttatttttcc caggtacatc agcggcatta ggagtaatat ttgctcaaca agcatctgaa
     8341 cttttaggaa tgagcccaaa taatatggca aatgtacttc ctatagcaat tggagttata
     8401 ctatttttag cgttattaaa tataataggt tcatccttag gaggaaaagt tcagacagta
     8461 gccactattg gaaagatgat acctttaatt ttaataatag tctttggatt tattaaaggt
     8521 caaagtagtg aagtattaaa tccatttgta ggagatggtg taaatgcatc aaatgcactt
     8581 ggacaagtat taatagctac tttgtttgca tatgatggtt ggataaatgt tggagctata
     8641 tcaggagaaa tgaagtctcc agagaaagat ttaccaagag caatagttgg tggtttatca
     8701 ttagttatgg cagtttatat tataataaat atagcttatc tttgggtagt accagcaagt
     8761 gaattagcaa cagttacttc cccagctaca ttagtagcaa caagattgtt tggaaatata
     8821 ggtggaaaag taataactgt gggtatatta atatcagtat ttgggacatt gaatggatat
     8881 ttattaacag gttcaaggat accttataca ttagctgaaa tgggaacttt accagcatct
     8941 aaaactcttt taaaagtaaa ttctggaggt tctcctgtaa attcaatact acttataact
     9001 gtgttagcct gtgtatatgc attatctggt caatttaatc ttttgacaga cttaacaata
     9061 ttttcaatat gggtatttta tgtattgact tttataggtg ttatgaggct tagaagacaa
     9121 aagccagatt tacacagacc atataaagtc cctttatatc caataatacc aattatagct
     9181 atattaggtg gactgtttgt aataataaat caaatattga cttcaacagt catatcttta
     9241 ggcggaatat ttataacact attaggttta ccagtttatt attatatgaa aaaaagataa
     9301 ctacttaatt taagttagat aaatttgggt aaaggtgaaa attaactttt atccaaattt
     9361 tttatactgt tatacaatat aagtattata tattgcttga tgtgctattt tgtttgattt
     9421 ataatttagt aattgatgtt ttaagataat ttaagaaatg aggagtgaaa gatgctagga
     9481 ctttatgtac atattccatt ttgtgtgaaa aaatgtaagt attgtgattt taattcttat
     9541 aaaatggaca tagattcaaa aaaaagatat atagaagact taaaaataga aatggaatta
     9601 tatagtaata aattatataa agataataaa tataaaaata aagaatgctg tagtctaaat
     9661 aaaaatgaca aaataacgag tatatttgta gggggaggta cacctagtat actaacctct
     9721 gatgaaataa gagaagtatt tataagtata aaagaaatgt ttgatataga tgagaatgca
     9781 gaaataacta tagagtgtaa tcctgggaca ttaacattag aaaaattaaa aactatgaaa
     9841 gagattggca taaatagact tagtataggg ctacaagcta ttcaagaaaa gcatttgaac
     9901 tttataggaa gaatacatac ttatgaagaa tttgagaaaa actataaaga tgcattaagt
     9961 gtaggtttta aaaatattaa tgtagattta atgtacagtc ttccaaatca aactctatgt
    10021 gattggaagg aaactcttga aaaagttgta cacttaaatc caacacatat atcagcttat
    10081 tcccttatac tagaagaagg aactgaacta tacaatatgt atgaaagtaa taaatttgaa
    10141 cttatagatg aaaatgtaga tatagaaatg tatgagtata caataaacta cttaaagtca
    10201 aaaggatata atcaatatga aatatcaaac tactcaaaag aaggctataa ttgtgagcat
    10261 aatattttat attgggaatg tgaacattat atagggattg gagcaggagc ttctggctat
    10321 attaatgaga acagatacaa taatgtagaa tctttagaag actatcattt aagtttggta
    10381 aaaagagaaa aaccaataca agaaaatgaa attctttctg agaaagatat gatagaagaa
    10441 aagatattta tggggcttag aatgaataaa ggcataaaat ttgaagattt taaaaaaaaa
    10501 tttggaatag attttagaga gaagtataat aagcaaatag aaatgctatt agctagaaag
    10561 cttataaatc agagctttga aggtatacaa ttaactcaaa aaggaagaga aatatctaat
    10621 agtgtattta tagaatttat ggaataattt ttaaggtgtt gacaaaataa atttataatg
    10681 gtatataata tatataatct ttttagcact cgaacttagt gagtgctaac agctaggagg
    10741 tataataata tggaattaaa tgaaagaaaa ttaaatatcc tcaaagctat agttaaagat
    10801 tatatagaaa cagctgaagc tataggttct agaacaatat ctaaaagaca tgatttaggt
    10861 gttagtgctg ctaccataag aaatgaaatg gctgaccttg aagaattagg gtatttaatt
    10921 cagcctcata cttctgcggg tagagttcca tctgaaaaag gctataagtt atatgtaaac
    10981 tctcttatga gtaaaagcga attggatgat aatgataaga ttcttattga acaatgtatg
    11041 aatcacaata taaatcatat aaaagaattg attcatgaaa cttctaagct attatcgcaa
    11101 ctaacaaact atacaactgt agcagttact aagagtttaa taaatcaaag tgttataaag
    11161 cacatacagt tggttgctat gaatgacaat aatattttac ttatagttgt aacagataaa
    11221 ggggacttaa aaaaagctaa ccttacaact aatgtatatt tagatcaatc taagttaaat
    11281 ttgatttctg ataatcttac aaggaagctc ttaggtaaaa gtataacaga tttggatgat
    11341 aatctaattg cttttattaa atacgaaata agtgagtatt ctgggcttat agatgagctt
    11401 ttaaatgctt tgaactcaaa tatgaaagaa gaagattttt ctctatcatt aaatggagct
    11461 actaacatat ttagttatcc agaatttaat gatgtattaa aagcaaagtc atttttaaat
    11521 atgttggaaa aaaaggaaac aatagctgac ataattaaat caaagggaat acaaaaagac
    11581 aatctaaata taataattgg tagtgataat gattgtgaac tggcacagga ttgtagtata
    11641 gttacagcaa cttataatgt agatagagac ttggttgggc gaataagctt tataggccct
    11701 accagaatgg attatgctag aatttactct ataataaatt atatgagttt attaattaat
    11761 agaaagtaaa gaaattttaa attgcgaggt gtaaagtctt ggataaaaaa aatgagcaac
    11821 aggaagtaag agaagaaaat gatacttcta ttaaccaaga atcggaaact caagtagagt
    11881 tagaagagga agttgttaat gaagaatgtg aaacatcaag tgaaaaaact gatgaaaaag
    11941 aagttgatga tgaaaatgta acagatataa attctaagtt agcagaaaaa aaattgcagg
    12001 atgagttgga tgaattaaat gataaatatc aaagacttca agccgaatat gcaaactaca
    12061 gaagaagaac tcaacaagag aaagagacaa taggcgtatt tgcaaatgaa aaaataataa
    12121 ctgaattgat accagttata gatagtatgg aaagagcctt agatgcatgt gaagataagg
    12181 aagatactat gtataagggt ataagccttg tacataaaca attaatagac actttagtaa
    12241 aatttggagt agaagaaata gaagcagaat ctaaggaatt tgaccctaat ctacatctag
    12301 cagttatgca agaaagtgta gatggagttg aagcaaatca aatagtaatg gtacttcaaa
    12361 aaggatataa gctaggaact aaagttgtaa gaccttcaat ggttaaagtt tcttgctaag
    12421 tataaattaa aggttaaatt atttaaccta atataaaata ttaaaataag taaaacttaa
    12481 ttatataatt actcgaggag gattttagtc atgggaaaaa taataggaat agatttagga
    12541 acaacaaatt catgtgttgc agtgttagaa ggtggagaag cacaaataat agcaaatagt
    12601 gaaggtatga gaactactcc atcagtagta gcatttacaa aagatggtga aagaatagtt
    12661 ggagaacctg caaaaagaca agcagttaca aatgcagata aaacaataac ttctataaaa
    12721 actcatatgg gaactgatta taaagtaaat atagatggaa aatcatatac tccacaagaa
    12781 atatcagcaa taatattaca aaaattaaaa tcagatgcag aaagttactt aggacaaaca
    12841 gtaacagaag cagttataac agtaccagct tactttacag atgctcaaag acaagcaact
    12901 aaagatgctg gtagaatagc agggcttgat gttaagagaa taataaatga gccaacagca
    12961 gcagctcttg cttatggtat ggataaatta gaccaagaaa agaaaatatt agtatttgac
    13021 ttaggtggag gaacttttga cgtatctata cttgaaatag gagatggaac ttttgaagtt
    13081 ttagctacag ctggtaacaa tagattaggt ggagatgact ttgaccaaat agtaatagat
    13141 tatttagcag aggaatttaa aaaagctgaa ggtgtagatt taagaaatga taaaatggct
    13201 cttcaaagat taaaagaagc agcagaaaag gctaagaaag aattatcatc aacaatgagt
    13261 tcaaacataa acttaccttt cataactgct acagcagaag gaccaaaaca cttaaatata
    13321 gatttatcaa gagctaaatt tgaagaatta actagaggtt tagttgaaaa aactatggaa
    13381 ccaactaaga cagctttaca agatgcagga ctttctacag gtgatataga tgatgtatta
    13441 ttagtaggtg gttctacaag aataccagca gtacaagaag ctgttaagaa gtttatagga
    13501 aaagaacctc ataaaggtat aaacccagat gagtgtgttg cagcaggtgc ttctatacaa
    13561 gctggagttt tagcagggga tgttaaagac ttattattac tagatgttac tccattatct
    13621 cttggtatag aaactatggg taatgtaatg actaagataa tagaaagaaa tactacaata
    13681 ccaactaaga agtctcaaat attctcaact gctgctgata accaaacagc tgttgatata
    13741 catgttcttc aaggtgaaag aagtatggct tatgacaata caactttagg aagattccaa
    13801 ttaacagata taccaccagc acaaagagga atacctcaaa tagaagttac ttttgatata
    13861 gatgcaaatg gtatagttaa tgtttcagct aaagatttag gtacaggaaa agagcaaaaa
    13921 ataactataa catcaaacac taatttatca gaagcagaaa tagagcaaaa gataaaagaa
    13981 gcagaaatga atgcagaagc tgacaaacaa aagaaagaaa aaatagaagc attcaatcaa
    14041 gctgaatcta ctatatatca aactgaaaaa actttaaatg aacttggaga taaaataagt
    14101 tcaggtgaaa aagaggatat agaaaaagct atagcagact taaaagctgt aaaagataat
    14161 caagatgcta cagcagaaga acttaagaaa gctacagatg aagtaatgac taagttccaa
    14221 aaagtatctc aagaaatgta ccaaaaagca gctcaagaac aacaagcggc acaaggtgca
    14281 gaacaagcac aagacaatgg acctaaagat gacaatgtag tagatgctga ctttaaagaa
    14341 gtagatgaag ataaataagc tatataatat gtaacactaa atgataattg gaacatatat
    14401 gatttgtgaa aatcatatag atgttaaaga tatatggaca atatacatca taaagaatat
    14461 aatatatcat agattatata acttaaagta gaatataaga attaatgata gcttgtagtt
    14521 tattattcta caagctatcg ttttgtaaaa tgaaggtggt gtcaattttt gagtacaaaa
    14581 agggattatt atgaagtctt aggtatcagt aaaggtgcag aagctcaaga gataaaaaaa
    14641 gcttatagaa aattagcaat gaagtaccat ccagatagaa atcctggaga taaagaggcg
    14701 gaagaaaaat ttaaagagat aaatgaagct tatgaagttc tatctgatga tacaaagaga
    14761 aaaacttatg accagtttgg acatgatggg ctaaatggtc aaggcggttt tggtggtcaa
    14821 ggaggatttg gcggtcaagg ctttgggggt tttgaagata tgttcggaga tatatttggt
    14881 gacatgtttg gaggaagctt cggaggtgga agagcaagaa gaagagggcc tcaacgtggt
    14941 gcagatataa gacagagtgt tactatatct tttgaagaag ctgcatttgg taaaaagatg
    15001 tctataaaag taaatagaag tgaagaatgt gaagaatgta atggaactgg agcaaaacca
    15061 ggaacatcaa agaaaacttg ttcaacttgt aatggaactg gtcaagttag aactgttcaa
    15121 agaactccat ttggtaatat agcaagttca agaccatgta gtgcttgtaa tggaactgga
    15181 gaagtgattg aatctccatg tagtaaatgt catggaactg gaaatacaag aaaagttaag
    15241 actatagaag tagatatacc tgctggtata gatgatggtc aaatgataaa actttctggt
    15301 caaggtgaag taggagaaaa aggtgctcca agaggagact tatatattgt agttaatgtt
    15361 aagtcacatc cactatttac tagagatgga aatgatattt attttgaaat gccaattaca
    15421 tttgttcaag caactttagg tgatgagata gaagtaccta ctttggatgg aaaagttaaa
    15481 tatagtgtgc cagaggggac acaaactgga actgtattta gacttaaaga gaaaggaatc
    15541 cctagaatta gaggtaattc aagaggagac caatatgtta aggttgtagt tgaaattcct
    15601 aaaaaattaa acgataagca aaaagaacta ttgagagaat ttgcgaagga gtgtgggtcg
    15661 aacgtccacg aaaagaagaa aaccttcgga caaaagatag aagatatgtt taagaaaaag
    15721 tagttaaagt agccaatata taatcatatt tttaaaatct tagttattta taagattgtc
    15781 aaaggagata ttatgatttt tatattggct atttttgtat aaaaaataaa tgtgcataaa
    15841 ataaaactga atataaataa ttttctgaaa aatacttgtc ctaaaatata taatatgcta
    15901 taatataatc aaataaaaga aaggggtgat tccagtggga acggatgtgg cactgccaaa
    15961 tgatgaagaa aactttattg taatatacaa aataaaatta gatactttta gtgctagatt
    16021 aagtactgga aaaactcatg agctgggata attattgtaa ttacctagac tcaaactttt
    16081 agtagtaaaa tagtaatatt atatagacaa ggtatctaat ttttaaatct tttgtatgaa
    16141 ttaaaatttt tataaagggt atatctgatt agaatgtaat tagggaaacc tagttgtgaa
    16201 ttacttttag tatatacatg tgtaaagaaa tatgtatatg gtattgggta ttgtgctaga
    16261 tgtgtatgta aaggaatatg tgtctagttg ggtatatgta aagaaatata cttataatct
    16321 atgaaaaatt tttagtataa attgctaaaa cattgagatt gttttttata gaaagttagt
    16381 ttataccaac taaaaatgtg cttaagattt gtttgatgtt taattataaa taaggttgta
    16441 gataaaaaaa taagtaaaga caaaggttta aaatacttgc tttaaatata gtttgttttg
    16501 aacacttgcc aaattaaaat aattaataaa atagacatct tctcatagta aaatatactg
    16561 tgagaggatg tttttgcttt gttcataaac ttttatgaaa aatttgcata gtgtcacatt
    16621 ttaatatact atatatataa taaaatatag ataaaattaa atgattcaaa agagaaaggt
    16681 gaaaagagat gtattatata ggtgtagaca taggaggaac aggtatacaa gcaggagtag
    16741 ttgataacta tgggaagata atttttaggt cagagtgtaa aacagtaatt gaaaaaggat
    16801 ttgaaggaat acttaatgac ataaaaataa tgatttataa gttgttggaa gataataaac
    16861 ttactatgag tgacataaaa tctattggat ttggtgttcc aggttttata aataaagaag
    16921 gattagtaac ttgtgtaaat ttaaaatgga ataaaaaagc ttttaataaa gagttaaaaa
    16981 gaagatttcc agatgttgaa attcatggag aaaatgatgc aacagtagca gcacttggag
    17041 aagcaaagtt tggaagtatg aaaggtgcta atgttggagt attatatact ttaggaactg
    17101 gtgtaggtgg aggaatagta attaatcaaa aagtcttttc tggagcacat ggattgggtt
    17161 cagaaatagg tcatcaaatt ataggagaaa actattttaa ttgtaattgt ggaaataatg
    17221 gttgtgtaga aactttttgt tcagcaactg caattataaa gtattctcaa aaactgatag
    17281 aagaaggaga aaagagtagg attttagatt tggcagaggg aaatttagaa aatgtaaatg
    17341 caaagatggt gtttgatgca tatagagaaa atgatttagt ggcaattaaa gtaataaata
    17401 gatttaaaga gtatttagca aaaacttttg caaacacaat aaattcttta gacccagaaa
    17461 ttatatctat aggaggaggc atttctaaat ctagcgatat aatacttgat ggaatagaag
    17521 gtttggtaag aaaatttgta ttatacaaaa cagaagatat agctacaatt acatgtgcaa
    17581 ctttaggttc agacgcagga attataggag cagctttttt ataatgtaaa tgaactgctt
    17641 attggaggcg tgatgatata tgggtaaaac aaatgctaga agaacgcttt ttttgatagc
    17701 aataggttca ggaactatgc tgaatcctct taattcttct atgatttcac ttgctttaca
    17761 tagtatacaa aatgattttc atattagttt tacaactgta tcttggattg tttcagcatt
    17821 ttacctagct agtgctattg cacaaccagt ttcaggtaag ataggagatt taataggtag
    17881 aaaggtctta tttttgagtg gtttgatgct tgtaatttta tcagcatcca tagtaccact
    17941 tgttcaatca ttctttatat tgatttttat aagagtaatt caagcaattg gaacaagtac
    18001 cctatatcca tctggagttg ctcttataca aaataatata aaagaaagac aatcttctgc
    18061 attagcagtt ctaaccattt ttgcttcaac tacagcagga ttaggtccga cacttggagg
    18121 attattgttg gacttaggag gatggcatgc tatatttctt gtaaatatac ctgttgtttt
    18181 ggtttctata tgcttagggt attttttatt cccaaaagaa gtaaaagaaa agaaatctat
    18241 aaaagaaact ttaaaaaatg ttttgtcaag attagatatt attggcatac ttctattttc
    18301 aattgcaatg atttttatat tactattttt gctatctatg aaggaatctt ttaacttaga
    18361 acaacttgta tttggtataa tactgatgtg tctttttatt tggcatgaat tgagaacaaa
    18421 attgccattt atagatatta agctttttgt aagtaatcct aaattgtcaa aagtttactt
    18481 gcaatttatt atattaaatt tctttaatta tattttgttt tttggtcttc ctagttattt
    18541 tcaagatgcc ttacattata gtgcaaagtc gactggatta tttatgctat ttatgtctgg
    18601 tataggtata tttatatctc cattaactgg aaaatggata gataaatctg gaactagatt
    18661 tcctcttgtt acatcatcaa tctttatgtt tattagtgca gtacttcttt cagtagtttt
    18721 tatccatata cctgtaatag gaaagggtat tatattatca ttagcaggta ttagttatgg
    18781 agttggtaat gtagccctgc aatcctctat gtttgaagaa agtcctcgtg acagtatagg
    18841 gacagcttct ggtctgtttc aaactagtcg ttatttaggt tctattttag caactgtttt
    18901 acttggaatg gtatttgaag taaatataac atcagaacaa tttcagatat taggttatgt
    18961 tatggtggtc ttaggtacaa taagtttttt attaaatttt atattaaaga aagaattaag
    19021 aaaagtagaa tagtggattt atggatttaa taagtctaat aactgataag tttaaaaata
    19081 ttgtttgtaa aacataactg gtttgatatt tattaattct aagttttgaa gcatagagta
    19141 ctcgataagt atacaatatt ttgaattaaa attaagcaga aatttgaact agtctttcaa
    19201 atctctgctt aatttagtag ttaaattttt ttatagagaa aataagattt gacacttaga
    19261 taaaaaatca ttgcattttc aaaattcttt aaatcaaacc caagcatctc attaatcttt
    19321 tccaatctat atactagcgt gtttctatgt aaaaacatac tagtagatgt cttagaaata
    19381 tttaaatcat tatttataaa ttttattcct gtatatagaa gttcttcgtc aatagattta
    19441 atcttattaa aatctattaa t
//
LOCUS       DAGSWI010000073        19109 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.073, whole genome shotgun sequence.
ACCESSION   DAGSWI010000073 DAGSWI010000000
VERSION     DAGSWI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.073"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(24..689)
                     /locus_tag="MJF80_002348"
     CDS             complement(24..689)
                     /locus_tag="MJF80_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281005.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            1033..2463
                     /locus_tag="MJF80_002349"
     CDS             1033..2463
                     /locus_tag="MJF80_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBZ0281006.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            2831..3409
                     /locus_tag="MJF80_002350"
     CDS             2831..3409
                     /locus_tag="MJF80_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBZ0281007.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            3439..3585
                     /locus_tag="MJF80_002351"
     CDS             3439..3585
                     /locus_tag="MJF80_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBZ0281008.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            3890..5269
                     /gene="scfB"
                     /locus_tag="MJF80_002352"
     CDS             3890..5269
                     /gene="scfB"
                     /locus_tag="MJF80_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBZ0281009.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            5298..5795
                     /locus_tag="MJF80_002353"
     CDS             5298..5795
                     /locus_tag="MJF80_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBZ0281010.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            6093..7757
                     /locus_tag="MJF80_002354"
     CDS             6093..7757
                     /locus_tag="MJF80_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBZ0281011.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            7770..10220
                     /gene="recJ"
                     /locus_tag="MJF80_002355"
     CDS             7770..10220
                     /gene="recJ"
                     /locus_tag="MJF80_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBZ0281012.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     NIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDVSLIDLDFNINYNIIEDDSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            10233..10745
                     /locus_tag="MJF80_002356"
     CDS             10233..10745
                     /locus_tag="MJF80_002356"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBZ0281013.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            10819..13026
                     /locus_tag="MJF80_002357"
     CDS             10819..13026
                     /locus_tag="MJF80_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBZ0281014.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            13051..13500
                     /locus_tag="MJF80_002358"
     CDS             13051..13500
                     /locus_tag="MJF80_002358"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBZ0281015.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            13515..14129
                     /locus_tag="MJF80_002359"
     CDS             13515..14129
                     /locus_tag="MJF80_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBZ0281016.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            14220..15776
                     /locus_tag="MJF80_002360"
     CDS             14220..15776
                     /locus_tag="MJF80_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBZ0281017.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNADNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFVVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            15766..17028
                     /locus_tag="MJF80_002361"
     CDS             15766..17028
                     /locus_tag="MJF80_002361"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBZ0281018.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEKANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            17102..18889
                     /gene="aspS"
                     /locus_tag="MJF80_002362"
     CDS             17102..18889
                     /gene="aspS"
                     /locus_tag="MJF80_002362"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBZ0281019.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETSPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         7735 a         1933 c         3474 g         5967 t
ORIGIN      
        1 taacataatt taaaattata ttattagtta agatttcctg ctttattagt aaataattct
       61 ttaatagcat tgtttctgga aactaaatcg ttatcagagt tgtttgtttt tttcataatc
      121 actgaataac gttttttatt tataaaaaat attaaaatct tatcatcatc aaaattgtat
      181 tctttatatc tgtcaataga ctcataacct agatttagaa tgtttttgtc ttttatacta
      241 tctacatcta aaatatatga tgggtcagcc tttagtaata tattaattaa acatttttct
      301 tcaccagcac agaataactc catcttactt gatatagatt tagattgctc tgaagttatt
      361 tttgcaggaa taaggttgtc attatcatct cttagaagat aaccatgtgt gtcttgttga
      421 tatactaaat taaccttttt cttaggaaca ctaatcattt tatctaaatg tacataatga
      481 tgattttcaa caaaaccttc atttaatatt tcaacaaagt tattaatttt acctaggcaa
      541 aaagattcta tagattcttt acagaattta tttgtaaatg gtattatgtt tacgttttcc
      601 tcgtctgtat tccatgctat agcataatcc aacgaatata cttcttcctc tatatctgca
      661 aaattatatt gtactatgtt tcctttcaaa attactcgac ctccatctta taatatattt
      721 aatatacttt ataaaactta tattatattt aaataatatc ataaattgga taaatatgat
      781 atagtaaata agaaaaaatt tgaaaaaaat cactataaat gagtaataaa tataaatagt
      841 gtatactaat gggatggaaa atgtccacta ttaactttat taccccacat atataagata
      901 aaattattaa tataaaatat aatctatcga caatgaatag acttttaaat aaacttaata
      961 taaaattaat taaataagtg cttaaaaacg atttacttta atgatagaat catattaaag
     1021 gaggataatt ttatggatat aaagaataaa attaaatcta tatttttacc agaaaaaaat
     1081 gactatttag atgatgaaga atttgagtat gatatagaaa ataatgaaga agatttaaag
     1141 gaagataaca aagtacattt aaatgattta agcagagtaa aatatgatta cagagagtta
     1201 aaagacatag aaaaccaaac agaagagatg tgtctgctag cagtaaagca ggatggtact
     1261 ataattgagt ttgtaaagga caagacttat aaagtatgta tggaagcagt aaaacagaca
     1321 tataaatctc ttaagtatat aacaaatcaa aatgaggata tttgtataga agcagtaaaa
     1381 cagaactaca gagctttgta ctacataaat aataagacag aaaatgtact aattgaggct
     1441 ataaaaaatg catcaactta tgatgtcatg gaagtattta aatttgtgga agagcaaaca
     1501 gaagatgtat gtctagcatt tatagaaagg gcatctaaaa atgatgttgc agagatatta
     1561 aaaggaataa aagagcaaac tccagccata tgtttagaag ctgtaaaaaa agatggaaaa
     1621 tctttagctt atgtaaagga acaaagtaat agtatatgtt tagaagctgt aaaagaaaac
     1681 tacagtgcac tatcttgtgt aaaagagcaa actgaagaaa tatgtataga agcagtaaaa
     1741 cagaatgact ttgcattgta ttatgtaaat gaacagacag aaaaaatatg tatggaagca
     1801 gtaaaaagaa gttatatggc tctacagtat gtaaataagc aaacagaaga gatttgtttg
     1861 gaagcagtaa gaattgatgg aagagcatta cagtacgtaa aagagcaaac agaagaaatt
     1921 tgcttggagt cagttagaca aaatggaaaa gtactgcaat atgtaaaaaa acagactgaa
     1981 aatatatgta tagaagcagt aagaggttca tttgaagaac ttgaaataaa agaaatatta
     2041 agttatgtaa agataccaac agaaagaata tttgtagaag cagtaaagca aaatggaaaa
     2101 attcttaaat atgtagaaaa tcagacagaa ttaatatgtt tagaggcagt aagagaaaac
     2161 tataatgctt tagcttatgt aaaggaacaa acagaaaaga tttgtttaga agcagttaat
     2221 caaagttatg aagcgcttaa atatgtgaaa gaacaaacag aagaagtttg tctaaaagca
     2281 gtaaaacaag attatagaat gttgaaatat gtaaataatc aaacagaaaa aatatgttta
     2341 gaagcagtaa agcaaaatta tagagcttta gaatttgttg ataatcaaac agaaaaagtt
     2401 tgtcttgaag cagtaaaaca gaatagaaaa gcactacagt atgtaaaaca aaaacaaagt
     2461 taaaaggcta aaatatatag aaaacatatg caaataaata aaatttgata ttttgtaata
     2521 aataaaatgg gtatctcaaa aggaactttt tagtttgtga ggtactcttt ttatatgaaa
     2581 tgaaatatat ttttagcctt ttaacaatgg ggaaaaggtt tatttctatt ttgacatagg
     2641 ctttttatgc atataaagct tgataaaatt gttttattat aattgttttg ttatataaat
     2701 aataagaaga aaattatata ggtctttttt tgaccagttt tacattttta acaactagtt
     2761 ttgtaaagta tattgaattt tctcaaaaaa gaatgtactc tatatatgta aaattttttt
     2821 ggaggtaaaa atgtttaaaa gatatgctga aaaaatgaca tctgttttaa tttgtaataa
     2881 tatgatagat aataatgaat ctaaagtcta ttcttatgga tttgaaatat taatagcttt
     2941 catagttaat ataaccacca tgctgtttat aggtttttta tttggtaaat ttacatatgt
     3001 attatttttt ttaatgtgtt attgtccaat aagacaattt tcaggagggt atcatgcaga
     3061 caattatttt agatgcttat taacctttat ttttataatt ttatctacaa tcttaattat
     3121 agaaaatata aatatagact tatttaaaaa tattattatg attattgcaa gtgttagttg
     3181 ggttggaatt tgtgttctgt gcccaataga acataggagt aaccctataa gtgataggga
     3241 aaaattggtt tataagaaaa ctgctatatt tatatctaca gtagtgttat tgataacaat
     3301 tttaagtctt tctttgagta tatttgtaga ttatttcaca tatagcgcat ttgccatgtt
     3361 ttggattttt gtgatgttag tattaggtaa gttaaaagct aaagtataag taaaatatgt
     3421 atgagggaga gttaagttat gaaaaagttt attgtaagat ttatgaagtt tgctagctca
     3481 ttggcactta gcactgctat tttgtcagct aatagtacct gtccttggat tattcatcag
     3541 cctaaagtac ccaaagaaat cagcaatcta aagaagacaa attaaataca agcacttatt
     3601 tatacaaaat tatcaggaaa aaagaaatta ttatatttta ataagattaa aacaatataa
     3661 aatcagatac aaaatttatt ttatttattt tataggagcc taatacctat ttggtaatat
     3721 attagtgcgc caatttaggg ggatatagtg tatttaatga aatgacttac ggagagtaag
     3781 tcatttttgt attgtatgta ttttatgtaa acttatgata taatgaaggg taaagtttaa
     3841 ttaaaataat aaaaaacttt agtatagata gtaatttagg aggtagggta tgagtatgat
     3901 acataaattt tctatgaatg gatataatat cgttttagat gtgaatggtg gagctgttca
     3961 tgtgttagat gatgttgcat acgatttgtt agatttttat aaagaaaaaa gtaaagaaga
     4021 aatattagag atattaaaat caaaatatca agaagaaaaa ataaatgaag catatgaaga
     4081 aatattaaat ttggaaaaag agggtctttt atatacagaa gatacatatc aatatcatcc
     4141 aagctttgta catagagagc ctgtagtaaa agctttatgt ttaaatgtag ctcatgattg
     4201 taacttaaaa tgtaaatact gttttgcagc tcaaggtgat tttggaggag aaaaagaact
     4261 tatgagtttt gaagttggta aggcagcaat tgactatttg attgctaatt caggaagtag
     4321 aaagaaccta gagatagatt tctttggtgg agagcctttg atgaactttg aggttgtaaa
     4381 acaactggtt gactatggaa gaagtgtaga aaaagattat aataaaaata taagatttac
     4441 aataactaca aatggtgtac tgctaaatga tgagattata gattatataa atgaaaatat
     4501 gcataatgta gttttaagtt tagatggaag aaaagaagtt aatgataata tgagaccaac
     4561 tttaaatgat aaaggaagtt atgatattac attaccacga tttaagaaac ttgtggaaaa
     4621 aagagcaaaa gataagtatt attatataag aggtactttt acaagagata atttagattt
     4681 ttcaaaagat gttatgcatt ttgcagattt aggatttaaa ttaacctctg tagagccagt
     4741 tgttggagat gagagtaatc catatgcact tagagaagaa gatttaccta agatatttga
     4801 agagtacgaa aaatttgctg tagagtatgc agatagacaa ttacaaggag atggatttaa
     4861 atttttccat tttatgatag acctaaatca aggtccatgt gtaataaaga gaataactgg
     4921 atgtggagct ggaaatgagt atctttctgt aactccaaat ggagatatat atccatgtca
     4981 tcaatttgtt ggaaatgaag aatttaaaat ggcaaatata tttgatgaag aaatagtact
     5041 tccagaaaat ttaaaaaata tgtttagaga agctcatgta tatactaaag aagagtgtaa
     5101 acaatgttgg aataagtttt actgttcagg tggatgtcat gcaaatgcga taaactttaa
     5161 taatgacata tcaaaaccat atgaattagg atgtgaaatg caaagaaaac gtacagagtg
     5221 ctctataatg atacaagcta agttaatgtt agaaggagca accaactaaa tttatatttt
     5281 aaaggagttg tttatatatg attagagatt atttagaaga taaaccttta attgacgaaa
     5341 gtgtatttgt ggcaaaatca gcagatgtaa ttggaaatgt taaaataggc aaagattcaa
     5401 gtatatggta caatgcagta gttaggggtg atgaaggacc aataactatt ggagaaaaca
     5461 ctaatataca agattgttct atagtgcatg gagatacaga aactataatt ggtaacaatg
     5521 taacagttgg gcataggtct atagttcatg gttgtaaaat tagtgataat gtgttaattg
     5581 gtatgggttc aataatattg gataatgctg aaataggaga atatactctg ataggagcag
     5641 gtacactaat tacatctaat aagaagtttc caccaggtgt tttgataatg ggttctcctg
     5701 gtaaggttgt tagagagctt acagaagaag ataaaaaata tattgatgaa tcttatgaat
     5761 ggtatttaga ggcagcacaa aatcaaaaat attagtttaa agagtggttg tttcaaaaca
     5821 tatttaaatc tgttttgagg caaccactaa tttattttat tagattttgt tgatatttta
     5881 gttttcaaaa agtatttgat taaaccagat ataaaatgtc aaaaataaaa attaactgag
     5941 ttaaataatt ttaataaaat tggtagtata tgtgataaaa ttaaatatta aagaggatat
     6001 aggatattag aatatcaatt ggtgaaataa tgtcatatac gataaattaa ggtgtaagtt
     6061 tttgggtata cctatcttag gagtgaaaag atttgagaat aagttataga aatttaaagc
     6121 gatatagaga aattggatat gttttaataa agtatggttt tagttttatt gtagaaagac
     6181 taaatataga aggaattgcc tataaaatac cactatttga cccaccagaa gaaataaaaa
     6241 atatgacaac tggtgagaga atgaaaagag ttttagaaga actaggacct acatatataa
     6301 aaataggaca gatattgagt acaagaaaag acttgttaga ccaggatata attgatgaaa
     6361 tatcaaaact cagagatgat gtagagaaat ttgattcaaa tatagctatt gatatattta
     6421 aagaagaagt gggtttaagt atagaagaaa tattcttaga atttaaagaa gaaccaatag
     6481 cagcagcttc aattggtcaa gtttatgaag gtgtacttaa aacgggagaa gaagtaatcg
     6541 taaaaattca aagaccaaat atagagaaaa taataaaatc tgatttagag attcttagaa
     6601 ctattgcaaa tactttaaaa gatttaaaaa aggattttaa tttagattta gtacagatga
     6661 ttgaagaatt tcaaactcaa ttaatgaggg aacttgatta tacatttgaa gctataaatg
     6721 caactaaatt ctctaggata tttaaaaata gtgatgaagt atatatacct aaagtatact
     6781 cagaatataa tactaagaaa attttagtaa tggagaaagt taatggtaca aagttaagtg
     6841 atgtagaaaa aataaggaga ctaggttata acacaaaaac tattgttgaa ataggagtta
     6901 ggtcattttt tacacaggta ttatctcatg gattttttca tgcagaccca catccaggta
     6961 atatattcgt agttgctaaa aataagatag catatataga ttttggaatg attggaatta
     7021 ttgataataa aactttaaac caattaaatg aaattgcatt agcaggtgta gaaaagaatg
     7081 ttgacaagat aatatatcta ttgatagaaa tggatgcatt aaatggagag gctgatatta
     7141 agggacttag acaggattta ctttatctaa tacattatta ctatgatatt tctatagaaa
     7201 aaataaatgt aacagatata ttaaatgaac tatttaggtt ttttagacag tataaaatag
     7261 tcatgcctgc acaatttgta actcttgcaa agacagttat aacattagaa ggaacaagta
     7321 gaactttaaa cacagatttt tcttttggtt ctatgggaaa agagtttatg aaacatcatt
     7381 ataagagtaa atttaaccct aaaaatgttg ttttaagttc aagacaaaat gtagaagaaa
     7441 tattactgga cataaaaact attccaaagc aattaaaagc aattttaaaa aatatagaaa
     7501 gaaataatat aaaaatgcaa attgaagatg ttaaaatgac gaggttagaa aattgtataa
     7561 tagagctaac atctcaaata tctttgagtt tagtgttggc atctattata gtaggttctt
     7621 ctcttataat tgcatctcca aatatagaaa ataatatatg gataaaattt actgcaatag
     7681 cgggtttctt tatatccttt ataataggcc tttgcttagt tataagaagt attaggtcaa
     7741 aatacaaaaa agattagggg gaattaaatt tgaaaaaatg gatattgaag tataaaggta
     7801 agatagagga aagtgaattt agcaaaaaac ttaatatttc cccggaaatc tgccaaattt
     7861 taaaaaatag agggatatgt actgaaaaag attcagaaat atttatgaat ccatctttag
     7921 attatttaag agaccctttt ttaatgaaag atatgcaaaa agctgttgat agaataaagt
     7981 tagcagttga gaagaacgaa aaaatttgga tttacggaga ttatgatgtt gatggtgtat
     8041 catctacatc tatcttgtgc ttatatttta agagtatagg ttacaatgtt gattactata
     8101 ttcctaatag attagaagaa gggtatggta taaatgaaga tgctattaag cttataagct
     8161 ctaggggatg tgacttgatt ataagtgttg actgtggaat tacatcagtg tctgaggtca
     8221 atacagcgaa tgatttgggc atagatgtta taattacaga ccatcatgaa tgtcaaagtg
     8281 aaattccatc agcctttgca gtaataaatc caaaacaaga agattgtaat tatccatttg
     8341 attcactatg tggatgtggt gtagcattta aaatgattca agccttgact ccaaaagaag
     8401 aatttaaaac tagtatgtat aattatttgg aaatagtaac actagcaact atttgtgata
     8461 ttgttccact tatagatgaa aatagaataa ttgtaaaaaa tggtttaaaa tcaatgaaag
     8521 aaggcaaaaa tattggatta agggagctta taaaagtttg tggtgtagaa agtgataaga
     8581 taggttcttc tcatataggt tttgcaatag gtcctaggat aaatgcttca ggtcgattgg
     8641 gatattctta cttaggagta gagctattta ctactcaatc acaagaagag gcagtagaaa
     8701 tagctagtat attggaagaa aaaaataatg aaagacagat gatagaagct aagatgtatc
     8761 atgaagctga agaaatgcta aagtctaata gcagatacaa tgatgacaag gtgttggttt
     8821 tagctaaaga gggttggcaa catggaataa taggaattgt agcatcaaag ttaactgaaa
     8881 aatactacaa accaacaatt cttctaggta tagaaaatgg ggaggctact ggttcagcga
     8941 ggtcaataaa aggatttaat atatttgaag ctctcataaa gtgcaaagac ttgatgacta
     9001 agtttggtgg tcatgaacaa gcagcagggt tatccttgga ttcagacaat gtagaaattt
     9061 tagcaaatga aataaataaa tttgcagatt ataatttaac tgaagatgat atgatagaga
     9121 atgtaaatgt tgagtttgaa ttacaagaaa atgtaataaa tttaaatctt gtagaagaat
     9181 tacataagtt agaacccttt ggattaaata atcctaatcc tagatttata gttagaaatt
     9241 atattttaaa agatttgaaa gttataggta agaatcaaca acatctgaaa ttaaacatag
     9301 aaaaagagaa aagctatgaa tgtataggat ttaatatgag tcatttaaaa tcaatgtata
     9361 aagtaggaga caaggtagat gtactattcc aattagatga aaataactat atgggaaata
     9421 ggaaagtaca atttttactt aaagatatac gattggctcg acctaaaagt gcaagtaatg
     9481 acaaactttc attgaaatta atgtcaaaaa taataccaaa agatactcaa agcctgtata
     9541 atatatctgt gtctgatttt gaattatttg atggaaatac agatataaat atatttgatt
     9601 attttgaaaa agatacacta ataatatcta attctataaa tggattttat agagcaatgt
     9661 cagatgtatc acttatagat ttggatttta acataaatta caatattatt gaagatgata
     9721 gcaaaaatac tgataaatta gagcttatat tttctcctaa tattgataaa atagacttaa
     9781 aaagatataa taatattatt ctttatgatt atttatacaa taaaggcgaa tattcgtata
     9841 tatatgagaa taaaagagaa gaaagtgaaa ttataaaata ttataataaa acagacctat
     9901 tatatttaaa gaatgtagtt agtaatatag ttccatcaag agatgaattt ataacaattt
     9961 ataaacaagc actcataaaa aaagaaattg atttggatat ggtaaatata agggaaactt
    10021 ttaatgtaat tccattaaag ttttttacga tattaaatgt atttagagaa ttaaatttac
    10081 ttgactttaa tttaaattat gaaaaaaatt ctgtactcat aagaatattg ccaaaaccac
    10141 agaaaaaatt agacttaaat gagagtttaa tactcaataa tctaaaaaat ttagaaaaac
    10201 aatacaatag cagttattag gaggcgtttc ccatggattt aaaaaacttt ataaggaata
    10261 ttgatgattt cccaaagcca ggcatagatt ttaaagatgt gacaactcta tttaaagatg
    10321 gagatgcatt taaatatgca gtggattcta tagttgaaga attgaaagat aaagatgtag
    10381 atttagtaat aggaccagaa gcaagaggat ttttgatggg tacacctgta gcatatgctt
    10441 taggagtagg atttgttcca attagaaaac ctggaaagtt accaggtgaa gttgagagtt
    10501 atgagtatgg attagagtat ggtacagata ctttagagat acataaggat gcaattaaaa
    10561 agggtcaaaa agtagcaata gtagatgatt tattggctac tggtggaact atggaagccg
    10621 ctgccaagct tgtagaaaaa ttaggtggag aagtagtttc tatgcaattt ttgattgagc
    10681 taaaattctt aaatgggaga gaaaagttgt ctaactatga tgttaattct cttataaaat
    10741 actagtactt ttataatagt tggtataaca ccaactattt tcatatatat taaaatttta
    10801 tacaaaatag gtggttatat gcatgataaa gaattacaag aaataattga aaaaataaaa
    10861 gtttatgcac ctaatgcaaa ttttgaaatg gttgaaaaag cctataatct tgcaaaaaaa
    10921 gcgcatgaag gacaatatag aaaatctggg gaaccatact ttattcatcc attagcagtt
    10981 gcaaatattt taatagagat ggaattagat attgaaacta taacagcagg tttacttcat
    11041 gatgttgtag aagatacaga atatacctat gaagacataa aaaaagaatt tggcaaagaa
    11101 gtagcggatt tggttgatgg agtaactaaa cttggtcaaa taaaatatcg ttcgaaagaa
    11161 gaaactcaat ctgaaaattt aagaaaaatg tttttggcaa tggcgaagga tataagggtt
    11221 atacttataa aacttgctga tagattgcac aacatgagaa cattaaaata catgcctcca
    11281 gaaaaagcaa agtataaagc aaaagagaca cttgaaattt atggtggtat agctcataga
    11341 ttaggaattt ccaagataaa gtgggaacta gaagacagag cattaagatt tatggaccca
    11401 gaagggtatt atgatttagt tagtagagta tcaatgaaga gaagtcaaag agaggattat
    11461 attcaaggaa ttgttgaact tcttaaagat aagtttaaag atttaagtat accatgtgat
    11521 gtatatggta gaccaaagca tttttacagt atatatagaa agatgcaaaa aaaacataag
    11581 acttttgaag agatttttga tttaacagca gtaaggatat tggttgataa tgtaaaggat
    11641 tgctatgcag tacttggtat agttcataca ttatggagac ctattccagg aagatttaaa
    11701 gattatatag caatgccaaa acccaatatg tatcaatcat tacacaccac tgttgtaggt
    11761 cctgatggag aacctttaga gatacaaata agaactcatg agatgcataa tatagctgaa
    11821 aatggtatag cagcccattg gaaatataaa gaaggtatct ctagcaaaga gtcaaaggtt
    11881 gaagaaaaac ttcaatggtt gagacagatg atggagtggg aaaaagattt aaaagaccca
    11941 caagagttta tggatgcact taaagaagat gtatttaata gtcaagttta tgtatttacg
    12001 ccaaaaggag acgtaattga attaccagca gaatcaacac ctatagattt tgcctatagg
    12061 gttcatacaa atgtaggtaa taaatgtgta ggagctaaaa ttgatggaag aatagttcct
    12121 attgattata agcttcaaaa tggtaatatt gtcgaggtac ttacatcatc taattctaat
    12181 ggaccaagta gggattggct taatatagtg aagaccccaa atgcaaaaag tagaattaga
    12241 caatttttca agaaggaaag aagagaagaa aatgttgacc gtggaaatac aatattagaa
    12301 agagaattta agaagcatga tataccacaa aaagaccctc ttgttgaaaa atatatggca
    12361 ttagttgcta agaaatttaa tcagccaagt gttgaggact taattgccac tgtaggttat
    12421 ggtggtataa tgtcatctca agtggtatct aaggtaaaag acctatattt aaaagaggtt
    12481 aaaaaagcta aaaaggaaca acagatacaa gaagaagatt taaataaaca caatataagt
    12541 gaaacagaat acagtaaaaa gagaaagaaa aacgcatcac aaggaattat agtaaaaggg
    12601 cttgacaata tcttagtaag atttgctaag tgttgtaatc ctcttccagg agatgaaatt
    12661 gtaggatata ttactaaggg taggggagta gccatacaca gagctgattg cccaaatgca
    12721 aacaataatg gtgatttctt ccaaaataga ttggtggagg tttcatggag ttcagcagga
    12781 aaaggtaagt ttgaagcgga aattcaaata aaagctattg atagaagagg aattgttaat
    12841 gaaataactc atattgttgc aatggataaa cttagtttaa acggaataaa tgcaagaaaa
    12901 ggtaaggata gtattgtaag tataaactta cttgtagagg ttaatgacac agaggaatta
    12961 aaaattctaa tgaaaaaatt aaaagcaatt cctggtgtag aaagtattta tagaatgaca
    13021 aattagagtt aaaataatag ggaggaaact atgagagcag tagtgcaaag agtatcttca
    13081 tctaaagtta cagtagatga aaacactata ggtcaaataa ataaggggtt actagtattg
    13141 ttaggagtga ctcatgatga taaatcaagt gatgtagatt atatgattga taaaatattg
    13201 aatctgagaa tttttgaaga tgaaaatgac aagatgaatt tatctctaat ggatataggt
    13261 ggagagcttt tagtagtatc tcaatttact ttatatggag attgcagaaa aggaagaaga
    13321 cctggattta gtaatgcagc tagaccagaa cttgcaaata acctttatga agaatttgta
    13381 aaaaaagcaa aagataaagg tgtgacagtt ggtacaggtc aatttgcagc tcatatgatg
    13441 gtagagttaa ctaatgatgg tccagtaaca atactattag actcaagtaa atctttttaa
    13501 gggggaaaac tttaatgatt ataaaaaaat ttgtagatag ttattttgga gtaaatactt
    13561 atgtattagg tgatgaaaag actaaaaaat gtgcagttat agacccaggt ggaagtttag
    13621 tagagacatt aagttttgta aaagaaaatg aattaaatat tgaatatatt atccttactc
    13681 atgggcatgg agaccatata ggatatgtta aggatattaa agaaaaaact ggtgctaaag
    13741 tagttgcaca tgttgatgaa aaagaattat taaatgacaa aaataaaaat ctaagttata
    13801 caatgagatg tggagcacaa gagtttgatg cagatatcta tgtaaatgat aaagacaagc
    13861 ttgaacttgg tgaattaaaa atgactttct tgcatactcc aggacatact caaggatgta
    13921 tgtgtataag agttgaagat gaaatgttca caggagatac tttgtttgct ggaagtattg
    13981 gtagaactga tttatatagt ggagacttta accaaatgga aaaatcactt aagaaattat
    14041 gcaaatatga agatgctgtt agagtatatc ctggacatgg accaaccagt actctagggg
    14101 ttgaaaagaa gacaaatcct tacatgtaat tttatattag tatttaaaaa agtataaaat
    14161 atttaataac tatattagtg agggggttct tctactgtta aatagaagga gttaagttga
    14221 tgttgtatgt ttttttaaaa ggacatgatt ataagtatga agtagcagaa cttataaaat
    14281 tatttaccag tgaatttaag tttaaagact ctaatgctga caataatata gagtcaaagc
    14341 atttaataaa tagattgatt tatgagaatg gtgtattatt ctcaagtact gaatactatg
    14401 aaaatggaaa cttaaagtac gagtcaatac agaatataga aaatatgaat cttgaatttt
    14461 tagatggatt tgatagtagc aaaacgattg aagaatttct taatagccta gatagtgaaa
    14521 gtaagaggaa ctttaaaaag ctgtgtaaag aaaatatcaa aaaaagcatg tttgtagtgc
    14581 ttaaacaagt atttaattct tatgtaccat ggggaatttt gactggtatt agaccagtca
    14641 aaattgtcca caatttaatg gataaaaagc tagatgataa tagcataaga accattctaa
    14701 aagataatta ttttataata gatgagaaaa tagatttagc tcttgaaata gctaaaagag
    14761 agcgattatt tatgtatcct atagataaaa ataaaatttc tctttatgta agtattccat
    14821 tttgtccaac tagatgtgta tattgttcat ttccttcaaa ttcattaaaa caatttggac
    14881 atttaaaaag agaatatgtt tataaattga ttgaagaaat taaggggttt gccaaagtta
    14941 ttaaggatac taagaaagaa gttgaaactc tatatattgg tggtggaact ccaactacac
    15001 ttgatgagga agaattggat ttgcttataa attcattgtt caatgaatta gatttaagta
    15061 aaattaaaga atttactgta gaagcaggta gacctgacac tattacagaa caaaaactaa
    15121 gagtgttaaa aaaacacaat gttagtagaa ttagtataaa tcctcaaact atgaatgatg
    15181 atactttagt aaagattgga agagagcata gagtatctga tttagtagat tgctttaata
    15241 tggctcgtag aatgggattt gataatataa atatggatat aattttaggt cttatagatg
    15301 aagacttaaa tatggtaaag aacacattgg aggagataaa aaaactttct cctgaaagcc
    15361 ttacagttca tacattagcc ataaagagag catccaactt aaatataaat atggataggt
    15421 ataaagaaca tctaacacaa tatgaagaaa tggtaaaaat gatagattta tcaatgaaat
    15481 atgcaaaaga tatgggatta aatccatatt atatgtacag acaaaaacat atgttgggaa
    15541 atttagaaaa tataggttat gctaaagaag gatatgagtg catttataat atacagatta
    15601 tggaagaaaa acaaagtaac tatgcattag gtgcaggtgc tatatcgaaa tttgtttatg
    15661 tagatgaaga tagaattgaa agagtagaaa atgtaaaaaa tgttgaacaa tacattgaaa
    15721 gagtagatga aatgataaaa aggaagaaag aagaggtata taaaaatgtt gactaaagct
    15781 cctagaggaa ctaaagacat aactccaaaa gaggcttaca aatggcgtta tgtagaaaat
    15841 aaatttagag aaatatgtgc tttatatgga tatgaagaaa tggtaacacc tatatttgaa
    15901 catacagaac ttttcaagag aagtgttgga gatactacag atatagttca aaaagaaatg
    15961 tattctttta aagataaagg tgatagagaa attaccctaa agccagaagg tacagcaggt
    16021 gtagtaagag catttataga aaataaatta tatgcagata ctcaaccaac aaaattattt
    16081 tatgtgacac cttgttttag atatgaaaga ccacaagctg gtagacaaag acaatttcac
    16141 caatttggaa tagaagcact tggaagtgat actccttcaa tggatgctga aataatcgca
    16201 ctagctgttc aattttttaa tgaagttgga ttaaatgatt tggtagtgag tataaattct
    16261 gttggttgtc cagtttgtag aaaagagtac aatgctttat taaaagagta tcttgactca
    16321 aaagctgata tattgtgtga tacttgcaat gagagaagag aaaaaaatcc aatgagggtt
    16381 atagactgta aaaatcctac atgtaaagaa aatatcaaag atataccatt tattgcagac
    16441 catttgtgtg atgattgtaa atctcacttt gataaacttc aagaatactt aaaggaaatg
    16501 aatataaact ttgtaattga taaaacaata gttagaggct tagattacta tagaaaaact
    16561 gcttttgaaa ttatatctaa tgatatagga gctcaaagta ctgtatgtgg tggaggaaga
    16621 tatgatggtc ttgttgaaca attaggagga ccaaaaggta taagtggaat tgggtttgga
    16681 ctaggaattg aaagactttt acttacactt gaaggtaatg gaattgaaat agaaaaccct
    16741 caatctacgg atatatttat agtaactata ggtgaaaaag caaatacaag aagctttaaa
    16801 ttgttaaaag acttgagaca aaatcatata agtgcagata aagaccatat agagagaagt
    16861 gttaaagcac aatttaaata ttcagataaa ataaattcaa aatttacaat agtaattgga
    16921 gatgatgaac ttaaaaatga cactgcgaca ttaaaaaata tgaaaacatc agagcaaact
    16981 actgtaaaat taagtacatt agttgaagaa ttaaagcaaa agctgtaaaa taagaaagta
    17041 tgatgttttg tgtattagat aaatatatag cattaaaatt agataaaatg gaggtagtat
    17101 cttggaaact ttaaagggtt taaagagaac tcactactgt ggagagttga gagaaaaaaa
    17161 cataaatgaa gaagttgtac ttatgggatg ggtacagaaa aaaagaaatt taggtggtct
    17221 tgtatttgtt gatttaagag atacaagtgg attatgtcaa atagtttttg atacagatgt
    17281 agataaagaa gcttttgaaa aagctgaaaa attaggggct gaatttgtaa tagctgtaaa
    17341 aggaaaagta tgcgaaagac aatctaaaaa ccctaatatg ccaactggtg atatagaaat
    17401 atttgcaact gaacttagac ttttaaacaa gtcagaaact tcgcctattt atataaaaga
    17461 tgatgatgat gtttctgaag cactaagatt aaaatataga tacttagatt taagaaaacc
    17521 atctatgcaa agaaatttaa aattaagaca taaagtaatg aatattacaa gaaattactt
    17581 atcaaacaat agattttgcg aaatagaaac accattttta atagctccaa caccagaagg
    17641 agcaagagat tatctagtac caagtagagt taatccagga aaattttacg cattaccaca
    17701 atcaccacaa ttatataaac aattattgat ggtaagtggt atggatagat atttccaaat
    17761 agttaaatgc tttagagatg aagacttaag agcagataga caaccagagt ttacacaaat
    17821 agactgcgaa atgtcttttg ttgagcaaga agatgttatg agtatgattg aaggtttgct
    17881 tgaagcgata tttaaagaag ttttagatgt agaattggca cttccacttc ctaagatgac
    17941 ttatgcagaa gctatgtcta aatatggttc agataaacca gatactagat ttggatatga
    18001 gttaacagat atatctgatg ttgtttgtaa ctgtggattt aaagtgtttg ctgatgctac
    18061 acaaccagga aaaagtgtta gaggtataaa cgtaaaaggc aaagctgacg actttactag
    18121 aaaacaaata tcatcactag aagaacatgc taagacatat agagcaaaag gtcttgcttg
    18181 gatgaaagta ggacaagaag gagtaacttc tccaatagca aaattcttca atgaagaaga
    18241 aatgaacgct atacttacaa gaatgaatgc tgaagtagga gatttacttt tatttgtagc
    18301 tgataaaaac tcaatagtat ttgatgcttt aggacaagtt agacttgaag tagcaaatag
    18361 gcttaatcta ttggataaaa atgtatataa cttactatgg gtaactgagt tcccagtttt
    18421 tgaagaagat gaagaaacag gcacattctc agcaatgcat cacccattta catcacctat
    18481 ggatgaagat ttagacaagt tagaagaagg tgataagtca tctttaagag cgaaagctta
    18541 tgatattgta ttgaatggtt atgaaatagg tggaggaagt gttagaatat caaattctga
    18601 tgttcaatca agaatgttta aagcacttgg ttttacagag gaaagagcta atgaaaagtt
    18661 tggttattta ttagaagcat ttaaatatgg tactcctcct catgctggtc tagcttttgg
    18721 tcttgataga cttgttatgt tacttgcagg aactgataat ataagagaag ttattgcatt
    18781 ccctaaaaat caaaatgctg tttgccctat gacaaatgct cctacattgg cggaagatga
    18841 gcaattagaa gaattaagta taaaagttga tataaaagat aatgaataat atgtagttgc
    18901 aagaaattaa aatgtaaaaa gtctggatat aaagtatatt tatattcaga ctttttttat
    18961 tagaattatt ttatattttt tctctatata ttttcataaa ttatctttgt attctcagcc
    19021 aatataatta aaatactatg tttaaaaatt ttacataaac ttggttgttt tagaaactac
    19081 ttctgtaatg agtacacgat tttaattaa
//
LOCUS       DAGSWI010000074        18897 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.074, whole genome shotgun sequence.
ACCESSION   DAGSWI010000074 DAGSWI010000000
VERSION     DAGSWI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.074"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(163..792)
                     /locus_tag="MJF80_002363"
     CDS             complement(163..792)
                     /locus_tag="MJF80_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281020.1"
                     /translation="MFEKKKKELFPWKISALILIGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            995..1318
                     /locus_tag="MJF80_002364"
     CDS             995..1318
                     /locus_tag="MJF80_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBZ0281021.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            1586..2440
                     /locus_tag="MJF80_002365"
     CDS             1586..2440
                     /locus_tag="MJF80_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281022.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(2626..4062)
                     /locus_tag="MJF80_002366"
     CDS             complement(2626..4062)
                     /locus_tag="MJF80_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBZ0281023.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNGGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(4133..5515)
                     /locus_tag="MJF80_002367"
     CDS             complement(4133..5515)
                     /locus_tag="MJF80_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBZ0281024.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(5651..6556)
                     /gene="garR"
                     /locus_tag="MJF80_002368"
     CDS             complement(5651..6556)
                     /gene="garR"
                     /locus_tag="MJF80_002368"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBZ0281025.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHDN"
     gene            6952..7623
                     /locus_tag="MJF80_002369"
     CDS             6952..7623
                     /locus_tag="MJF80_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0281026.1"
                     /translation="MATFKRLQEQAYDYLKELILSGKMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            7726..8274
                     /locus_tag="MJF80_002370"
     CDS             7726..8274
                     /locus_tag="MJF80_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281027.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            8536..8844
                     /locus_tag="MJF80_002371"
     CDS             8536..8844
                     /locus_tag="MJF80_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281028.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(9006..10796)
                     /locus_tag="MJF80_002372"
     CDS             complement(9006..10796)
                     /locus_tag="MJF80_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281029.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(10796..12523)
                     /locus_tag="MJF80_002373"
     CDS             complement(10796..12523)
                     /locus_tag="MJF80_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281030.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RSKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(12768..13034)
                     /locus_tag="MJF80_002374"
     CDS             complement(12768..13034)
                     /locus_tag="MJF80_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HBZ0281031.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            13332..14444
                     /locus_tag="MJF80_002375"
     CDS             13332..14444
                     /locus_tag="MJF80_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBZ0281032.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(14808..15317)
                     /locus_tag="MJF80_002376"
     CDS             complement(14808..15317)
                     /locus_tag="MJF80_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBZ0281033.1"
                     /translation="MSQQNTGNAIVNSKPKKTSVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(15319..15966)
                     /locus_tag="MJF80_002377"
     CDS             complement(15319..15966)
                     /locus_tag="MJF80_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281034.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(15980..17152)
                     /locus_tag="MJF80_002378"
     CDS             complement(15980..17152)
                     /locus_tag="MJF80_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBZ0281035.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAE
                     RLKLEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            17420..18796
                     /locus_tag="MJF80_002379"
     CDS             17420..18796
                     /locus_tag="MJF80_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281036.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
BASE COUNT         6536 a         3076 c         2272 g         7013 t
ORIGIN      
        1 gcaagatttt acctcaatat aacttttata tttattacaa ataaattatt gtatttttat
       61 aggaattaaa tgtgactaca attttaatac taatctattt aatattaaat tatttattat
      121 taacttactt agtatttaac ttgaaataat attaatttac tattaacttg caaagtcttc
      181 taatcttgaa taggcctctt cttcagtatc cataactaca ccttttttga tttcatcttg
      241 aactgtttta ggaagtgaat ctactcttat ttttgttttt tctactaatg ttttacttcc
      301 gctatcatca tatttatata gtgctatatt tccattatca ttttcaatag tatatttacc
      361 ttttatagta acatccattg cttgtacatc tttattatca ctatatttag attctaccaa
      421 tatgatttca tttttatcca tactctttat ttttttagtt ggatattctt ttttaaaata
      481 tgatactatt tctgacttcg tcttatctaa taatgactct ggaactattc ctgccattgt
      541 aagtgttctc ttatagttag tttcattttc gccttcactt tgaagccaaa cttcacaatt
      601 tttatttaat ccaaaagctt cttttgactt ttcattcata ttcgcactag tacttacaac
      661 ttcaccagtt tgaactccat tgtgttttaa tcttgtccct atataaactc cactacttaa
      721 tacaaatact ccaatcagta ttaaggcaga tattttccaa ggaaataatt cttttttctt
      781 cttttcaaac attgattacg cctccttttt ctattatttt ctccaagctg taagttttta
      841 tacaatttag gtaaatttta gaagaaaata tatttatatt tcaaattact ctaagaatag
      901 atattttcta aaaggtagtt ttctcttaat ggttatattc ttgtaattat atggacataa
      961 tttataataa ataatcgttt aggaggtaat aactttggaa agaataaact gtaatgtaat
     1021 taactgttca cataacaaca gccatatatg ttatgcagat aaaattttta taaatggtga
     1081 aaaagctcac aatgacagac atacttgttg tagttcattt ttagatgaat ctatttatag
     1141 taatttatct agcaacgtta ataatagaaa ttgtgaggaa ttagtttgta ctgtaaaaaa
     1201 ttgtaaatac aacaaagatg gtagcttatg tacacttgat aatatattgg ttgctcctga
     1261 aactgataga gtaaatcttt atattgaaac atgttgctct agcttcgaat gtaaatagtc
     1321 atagtataaa tataagggtg tcaaatgtaa ttaaactaac atagtgtaga gtatctacaa
     1381 ctattagttt gattatactt gacaccctta tttattttaa ataaaatttc attattataa
     1441 ccatgtttat aatgttctaa ctgtaattaa atttgataaa tttctataaa ttggtataat
     1501 caatatgtaa attgaattta gtaaattaat cataaaaata aaatatcgag tatatagtgt
     1561 tatactaact agaaatgagg tgcttatgag tacattttat attcaatttc tttattttgt
     1621 aatttatagc tttataggat ggtgttgtga aactacatat tgttcagttt tacaaaaaca
     1681 atttgtaaat agaggttttt taaatggtcc attttgccct atatatggat ttggagcatt
     1741 agcaatagtt gctaccttga ctccttttgt acacaatatt cctttgttat ttttgtttag
     1801 tataattata actagcataa tggagtactg tacgagcttt attttagaaa aaatatttaa
     1861 tatgacttgg tgggattatt ctaaacataa atttaatatt catggaagag tttgtctgga
     1921 aaactcactt atgtttggtg tattatcatt gattgtaatg cttatagtac atcctattgt
     1981 ggttgacttt attaactcta tctctaaaaa tgtgctattt atatttgcta tatctataga
     2041 aatttatttt gttttagatt tagttatcac agtccataca attttacaat taaatggtaa
     2101 gttaaagcaa atcaatctta taattaagga attaaaagat aagaaagaat actacaaact
     2161 tattacacaa gaaactatag aaaacaaact tgaatcttta gttgaaaata gactggatac
     2221 tttggaagat aggtttgaaa tacttaagga aaaagaaaac tatcgctatg ctaaaaatcg
     2281 tattgatgag ctaaaaaata aacttaatac tttgctgtct aaccacaaat tacttcatag
     2341 acgtataatc aaagcattcc ccaacataag ctctaataag catacagata ttttaaataa
     2401 aatcaaagaa aatattaaat caattaaaaa agataaataa taaaataaac acataagtta
     2461 aataataaaa atactaatac ataagattaa ggtttagaat attttttgta aaatactcta
     2521 aaccctaatt aatataccat tatttttaat cttaatttat aattttagtt tattttactt
     2581 ttaacacagt aaatttaagc gtttaaactt caatacacta atatactacc ccatcaatat
     2641 ctcaactgat tctctaagtg tgctaatctc tcccacattt ccagggaaaa ttatatatgg
     2701 catattagga aatttgcttt ctttgcctgt catccatact ggtataccag gttttatctg
     2761 tcccattact gtagcttttt ttactcttag tgctttagta ccaacatcac tagaagtaat
     2821 tccaccctta gctataataa aatttggtct tacattaagc ataccaatta tactggtaac
     2881 tgcatctgat atttctacag aaatcataag ctgtttatct ttatcattcg tatccaagtc
     2941 aaaacgctct cgtctagtat aaacagcaac agttttacca ctgcttattt tttcctctac
     3001 aacttttata actcttttta cctcattttt tagaccattt tcttgaagaa ctaaatgttg
     3061 attaaattct ataaattcta tcggatattt acaattttta agttcttcta actgttgggt
     3121 agtcttattc acatgtgaac caacaagtat aattcctcca ttggtattac cttttgatac
     3181 taattcatcc ttagtaagta gttccttgtc actcactccg ccaagtactt ttgttatggc
     3241 agcagcgctt ctaaacataa attccttacc accatttact gctcgtataa acgcaattgt
     3301 aaataccttt acatctacat aatcggttgc atttacaata attttattga atccttcagc
     3361 ttttttaagt ttttctgtta tagcttcaat atttagctct ctaagttctt ttgacgaaat
     3421 atatatcata tcactagatt tgtactttcc atttgtcttt tcttcacacc actcacctat
     3481 atcagatgat ttatatccaa aagatttatc ttttgcaaat tcagtcattc ctgctggtac
     3541 aagtatttct ccttctttaa cataatgtac attatctata gtaaacctac caccttcttt
     3601 aaaaaatggc attattattt caccctgaaa taacttcccc gataatctct ctacttcgct
     3661 ttttaataaa ttagtttcaa gaggataatg tcctctcata gtagaatcac tacgacttat
     3721 gattataaaa tccttattaa ctttctttga tacatccaca atattctttg aaatttcttt
     3781 atgtgctttt gtagtttgag caacagtaaa tccccttgaa ttggtaagta taaaaaacat
     3841 actatttttc tcattaaagc cttgttccat actatcctta tcccaatcag tataaacaga
     3901 aatatcatga actgtttgta cacctgttgg gtcatcatct aatacaacaa tttttctatc
     3961 taaatgctta agctcatctg caagaagttt atttaatcta tcctcatcta cttgtttaaa
     4021 agtattaagt atatctatgc taattggttt tacttctctc atttaaaacg cccctttatg
     4081 taatttgtat agtctaaata taatttatat gatataaaca atagtttgtt atttatagac
     4141 ctggaagtgg tataattcca tttataacac taagcaataa aaccattata aatgctacaa
     4201 ctccagcaat ggttcctcca attgtccaag agtttaaagt atctttaact gataatccac
     4261 taaatcttga aagtacccag aatccagaat catttggaag ggagaaacca actcctccag
     4321 cacatatagc aagccctact aaaactggtg acattccagt tgccaaaatt gtaggtccta
     4381 aaattgatga tgttgttact aaggctacag tagcagaacc ttgagaaatt ctaagcaagg
     4441 cacttaataa gaatccaagt actacaactg atatactaag accagacata gtatcaacta
     4501 agaagttacc tataccactt gcatttataa cactgccaaa cgcaccacct gaccctgtta
     4561 tcaataatat tagccctgct gaatcagcag cttcaataac tacatctccc ataggtttat
     4621 ttatgtattt ctttaaaaat cctattgcca ccaatattcc aagaagtatt gctatgtttt
     4681 tatctcctat aaatgcaagt actccactca ttgtagaacc tttttctaag aacatactaa
     4741 atacatttga tataagtata agaagtattg ggaacaataa agaaaacata gaaattccaa
     4801 aacctggtac tttagaagaa tcatcatctt caatttttat gctatctttt ccctgctcta
     4861 aatttttttc tctatcattc tcatcaatag cataagctgg atatttttca aaacgttttc
     4921 cataaatcca tccacctata agagctgctg gaaaagcaac aattatagaa taaagtataa
     4981 aactacctac atttgcattt acatttgctg caactgccaa aggtcctggt gtaggtatta
     5041 caagtgcatg acctactata gtcccaactc ctaaggcaca tacaaaaacc attagagatt
     5101 tcttagttac tctcgaaaca tacttaatta taggcatcat tataacaaaa gctgcatcca
     5161 taaaaactgg aattgaaatt aaaaaacctg ctgtagttac tgctaaagca ctgttttttt
     5221 ctccagtctt agctaacaac ccctttgcaa tagattctgt tgctcttgat tctgaaagaa
     5281 tatttccaaa tattattcca agtccaatta caatacctat actacctaat gtatttccaa
     5341 atccagtcga tattgtggtt gatatttcat ttataggcat tctaaccata aagcctgtaa
     5401 ttatagatgt tatcagtaat gccaaaaacg gatttacttt aaatttaata atcgatacaa
     5461 gtaaaattcc tatagatata acaaatgcta tcaaaagtcc atatcctgta atcataatct
     5521 ttaccccttt atcattctac tttattattt atcaatttta tttttctttt actatgataa
     5581 cttaacctcg ctgtatttat ataatctaat cctaataata tgtatttaat tatattgata
     5641 tatttaaata ttaattatcg tgttttttta tttcaacacc tgccaacttt tcaaaatact
     5701 gtgctattcc actatggtca tcttccaaat gtccatgtac ttttaatgtc tgcataattt
     5761 ctaataattg acttgtcata ggtagtggta catctaaatt gtgtgctgtt tgcattacat
     5821 tcttaatatc tttcaaatta atagatattt ttccacctgg tttaaaatca cgattcataa
     5881 tcattggtat cttcgcatcc aatattgtac taccagctag gcctcctctt attgccttat
     5941 atactttttc tgggtctgca cctgcttttg ctgcaagtac aaatgcctca gaaactgctg
     6001 ctatcgtcaa attaactatt acttggtttg taagttttgt aacacttcca ctaccattat
     6061 ctccaattaa aatagatgag cttcccatta tattaaaaaa tggctctact ttcttataaa
     6121 tttcttcctt accaccagcc ataaaagcaa gtgtaccatc aatcgctttt ggctcccctc
     6181 cacttactgg tgagtctata aattctaagc ccatatcttt taatttattt gcacacaata
     6241 tggattctgt aggagtaact gaactcatat cgactactat acttccttct tttaaagttt
     6301 ttgccaatcc atccatccca aacaatatat cctgcacaat agttccattt ggaaggattg
     6361 taaatacaat atcacattct tgacccattt ctaaaacact tgcgtatttt gctccacatg
     6421 atataagttc atccacagca gatttattaa tgtcatacac caaaagatta catccatctt
     6481 ttaataaatt ttttgccatt ggctttccca ttattcctaa tccaataaat cccaatttca
     6541 ttttataacc ctccatatct tcacattttt gaaaacgttt tttatgtttt agagtatact
     6601 acataccagt ataccggtca atataaaata taaaagtatt tattttaaca ataaatagta
     6661 atttttaaag tcatttatta tatttagtca tcctatataa gttttaatac atactattgt
     6721 tttcaaatat aatatactac ttttacagtt ttgtatttta actaatttca attagtttta
     6781 attaatctgc ctttaatgtt aatcatgctt cacacttcaa taatttttta tattttttaa
     6841 tcttatctgc aatttaaatt gcaaaagatt gaccttattt tcactatgtt gtatgcttaa
     6901 aattatataa gtttttgtat ttttatattg tttagggggg tattattaag tatggctaca
     6961 tttaaaagat tacaagaaca agcatatgac tacttaaaag aattgatact atctggaaaa
     7021 atgatagaaa atgaaatata ttctgaaaca aaacttgcta gtgaaatagg tatctctaga
     7081 acacctataa gagatgctct tcaaagactt agtcaagatg gatttataga tataatccca
     7141 agcaaaggat ttagaatcca tcaaattaca gcaaatgaaa ttgtagagat attccaaata
     7201 cgctctgcaa tagaaggatt ttgtactttt ttaattacaa gtcaatacaa agaagctaga
     7261 gcagtagaaa ctataagtaa attaaaacat cttttagata aacaaaaaga tattttatta
     7321 ggtgataaaa atttaaactc atttgcagaa tacgatacat tatttcacac aactatagtt
     7381 tcatatgctc aaaatactga atttgacaaa atgtttaaca attatatgta cagaataaaa
     7441 aaattagctc tcgattcatt gtcacatgaa ggaaggcttg aaacaacatt aaaagagcac
     7501 acagatattt ttaataatat tgcaaatgga tgtattgaag atatttacag aactactctc
     7561 attcatatgg aaactcctaa atacattaat ttagaagatt tttgtaacaa taagttattt
     7621 tagcattata ctttcattta ttaaaaatat atctatctca aattaatttc ctaatttata
     7681 aaaaatattc tataattata ttgtaactaa tacttattct aaggagtgat ttttttgaac
     7741 gaggatgttg taactaatga aactatcgac aaagattatg ctattgaaga agttcgtatg
     7801 gcatgcaaac attttggtga tttatatttt tatttttcca aagtgttatt tgaagagttt
     7861 ggtgaagata aaactagcga aatattaaga aaagttttat ttgaaaggtc tgaagaaaga
     7921 gctattgcta tgagagaaag agctcatgaa aatggagatg aattaatcgc agacaatatt
     7981 attagcacaa ctgatgttcc atttctagga tgggttcctg aatttgaaga gttacattgc
     8041 ccctatggag cttcttggct tttaagattt gaggaaaatc cttggtttaa aaaatttgct
     8101 tctctttact gtgatgttac agatactact gtagctgaag tatttacagg agatacgtcc
     8161 cataaaataa caaaaaacat tctatgggga gataaaagtt gtgaaagaat atatttccat
     8221 gatgacaagg tatctgaagg taaatatact tatggaaata aaaactgtaa ataaataatt
     8281 taaattttct atattcatat actatatttt gtactttttc atataaatat tataactagt
     8341 cttttatttt acttttgttt aacctaaaga taatttactt ttaatctata tgaattatca
     8401 tataaataat cagaaagtta tctttataaa ttatagtttt agatttataa tttataaaat
     8461 atttatgttc tttgtaaaat tttttagaag cttctaatgt tatgctataa aactatcttg
     8521 agaagggctg attgaatgtt taataaaata tatgaaaaga aagaatttat agtttttcaa
     8581 gttaaaaaag gttatgtagt ttataatact agaaagagtt ttgaagaagg tcatacacat
     8641 ttaaagcatt ttgaagcagc aaagacagct attgatttag caataaataa aaagattcct
     8701 agaagcaaag atggatatta tctgactagt ttaattcgca taagtgatga tggttattac
     8761 attgataagt taagcgaatt gttatatgtt agagaacaaa aaggtaaaaa agaaaagtac
     8821 tgtaattctg gttatcagat gtaacaagac tagttgtgaa ttataagtac caatcataag
     8881 tacctttagt cttaaaatca aagagtatca gtaaatctat aaaaaaacaa cttactgata
     8941 ctctttttaa ttatatgatt aatatttgaa tttgatacaa aattttatac aaaagtttat
     9001 gttatctatt ctgattcttt gtctgcaaat tggctattat aaagcttctc ataaaatcca
     9061 cccttttcca ttaattcatc atggttcccc tgctctataa tacttccatt attcataact
     9121 aaaatcaagt cagcattgcg aattgtagaa agtctatgtg ctataacaaa actagttcta
     9181 ccattcataa gatttctcat tgctttttga agcataagtt ccagcctagt atcaacagaa
     9241 ctcgtagctt catcaagtat caatattgaa gggtctgata taaaagctct agctatggtt
     9301 aaaagctgtt tttcccctag agatatatta gatgcttctt catttaaaaa catattgtat
     9361 ccatctggaa gagtggttat aaagtgatga acatttgcta cttttgctgc ttgtataatt
     9421 tcttctttag tagccccaaa tctaccatat tctatatttt caaaaatggt tccattaaat
     9481 aaccatgtat cttgaagcac cataccaaac attgaacgca aatctttacg tttcatatct
     9541 cttatatcta ctccatcaat tttaatagca cctttcttta catcataaaa cctcatcaat
     9601 aagtttatta atgttgtttt acctgctcca gtaggtccaa caatagctac catctgacca
     9661 ctttttactt ctgcatttaa atcttctatt aaagttgaat tttctccata tccaaaatta
     9721 acatgctcaa atgtaacatt tccttttaca ttttctatct taactggatt ttcaacatca
     9781 ggtatttcct cttcttcgtc taaaatttca aatacacgtt ctatagctgc aaaagaagat
     9841 tgtattactg tagataactg agtcatttgt gaaagtggct gatttatctg ccatatataa
     9901 cgcacaaatg cttgtaaatt tcctactgtt attcctccac ttactgcata tatagaaccg
     9961 acaactgcca cagctgcaat tccaaagtaa gttactaatg aaaccatagg attcatcata
    10021 cttgatataa attgtgcctt aaatccattt tcacaaagtt cttgatttac ttttttaaat
    10081 tcttttatag aatcttcttc tttaccatat agttttattt cattaaatcc agtatacatt
    10141 tcttgtactt tcccatttaa ttttccaaga gcattttgtt gcaatgaaaa tctactttga
    10201 gattttttta ctacaaattt agatacaaaa taactaattg gaattataaa aagagctata
    10261 agagccatat atatattaat tgtaaacatc attgaaagtg ccaaaattat gactaagatt
    10321 gagtttacaa tttgcataaa actctgttgc aatgcatttg atattgtatc tacatcattt
    10381 gatatacgac ttaatacatc tccaaaacta ttactatcaa aataactaat aggcaatctt
    10441 cttattttat tttcgacttc atttcttaaa tctctcattg tgttttgaat agcattcgta
    10501 agtagaaaac ttgaagtata cgtaaaaatt gcattggcaa aatatataca tgctaaaatc
    10561 accaatattt ttgttacata atcaaaattt acggaagccc ctggtactcc ttttgcgata
    10621 ctaattacat ctttagtcaa ttgtgttatt atcaatcctt cagttttagg tgcaagtgca
    10681 ttcatagtag ctgctgaaat tatacatata atagctataa taaaggaaaa tttatatttc
    10741 tttacaaatg gaagaagtct accaattgtt ttcttaaaac ctttagactt tttcattatt
    10801 ctaattcctc cttttttaac tgagatgttg caatttcata gtatatttca cagtttttta
    10861 ataattcttt atgagttccc attcctacaa cttgtccttc attgagtact ataatcttat
    10921 cagcatcaat aatagaacta attctctggg caacaatcaa tacaattgca tcttttgttt
    10981 ccttcttcaa tttagaacga agtattgcat cagttttaaa gtcaagtgca gaaaaactat
    11041 catcaaaaat gtatatttca ggtcttctta taatagccct tgcaatagaa agtctttgtt
    11101 tttgacctcc tgatacattt gctccacctt cactaatcaa ttcatcaaac tttctaggtt
    11161 tttttgatat aaactcatat gcttgtgcaa cttttgcgga atattctaat tctgactctc
    11221 ctgccttatg tttcccaaat ctaatattat ttgctatgct accagtaaaa agcagtgatt
    11281 tttgaggtat gaatcctatc ttttttctta aagcttttaa atcatattct cttatatcta
    11341 ctccatttat cttaatgcta ccttctgtta catcataaaa tctaggtatc aagttaatta
    11401 gagtactttt cccactacca gtacttccta taaaagcgac tgtttcacct gtttttgcag
    11461 taaatgaaat atccttaagt acagaagctt ctccatctgg ataggtaaaa gttacattat
    11521 caaattctat gataccctta ttttcagtat ccttaattcc attttcagga ttttttatca
    11581 atggttcttc atttaaaagt tcttttattc tatttgcaga tacctctgct cttggataca
    11641 taataaatac cattgaaaaa agcataattg aaaacatggc atggaaaaga tattccaaaa
    11701 atgcaaccaa ctgtccaact tgaagttctc caatatttat cttctcactt gaaatccaaa
    11761 atacagccaa aacagctatg tttaatagta aaaagaatgc tggttgagta attgacataa
    11821 gcttaaataa tttcttagaa acattagcat aatatgtatt tgttttttca aatcgctcgg
    11881 tttcataatc atcatttcca aaagctctaa ttactcttat tccagttaaa ttttctctcg
    11941 atataaggtt taatttatct agtccttttt gttgttttga agatattgcc tgagatactt
    12001 ttgctattat tgctactcca ataattataa aaggtactga tatagccaat actatagaaa
    12061 gttccacact tgttctaatg gtcattatta tacttataat gaacataact ggagttaata
    12121 aggcagtcct tagtagtata ttaacaaatt gttgaatttg aaatgcatca ttcgttgttc
    12181 ttgtaatcat tgaagaaatt ccaaatttat tgtattcagt atgtgaaaat tcttgtgatt
    12241 tctcaaatat gtcatttctt atatctctgg ttacacttgt tgatacctta gcagaacaat
    12301 aacccaacaa tatactacct acaaccccta taatagaaat aataacaatc actattccca
    12361 tttgttttat gtaagctata ttctgatttg caataccatt gtcaattatt ttagctatta
    12421 tagttggaat ccccaactcg actaatgcaa aactaaatat agccagtatg tttaatacta
    12481 ttaataactt ataattcttt aaataactta aaattaattt catatttttc tcctttctta
    12541 atttttgaga tagtgtaatc atgtaataat actaaattta taaatgctta attttataaa
    12601 aaaaggagaa aagagttaac gcactctctt ctccagcata ttctttttaa tacaccctcc
    12661 gacaagtcgc ttatatgtta acatatttga caagtaatgt caattcatat agccaaaagt
    12721 cttttaaatg cttcgtttca taacattgta acaaattggc atgtaattta tttttgtttt
    12781 ttactgtatt ccaattttac aagttctcca atattactag ctttcttaaa attcaaatta
    12841 gtcttatcta taccagactt aaatagtgaa atccctctcc ccaatattat tggcaatata
    12901 tatatataat aatcatcaat taagttatct ttcataaata attttactat atcgcctcca
    12961 ccaactatcc aaatatcttt tttatccttc tcttttccaa tttgctcaat taatttttta
    13021 ggttctaaat taatttatag tgtgattatt ctatgtaaac atcacttttt tactcattca
    13081 ataataccct attaataaat cctcaaacta atttaaataa actttgcata aaattaaaaa
    13141 tatattatat aaataattta aaactacact aacttattga aaattctaat tattacaaat
    13201 aaatattgct ctctgttaac gctagttcta taatataatt ataagctatt aatattataa
    13261 agccgatatt actaatataa taattataat acttatagtt aatttacttt tgttgaaagg
    13321 tggtaaataa catgaaaatt tcagaagtcg ctactccaaa ttttttgcta gatttagacc
    13381 agttagaaaa gaatatagat aaaattcaac aaatttgtac aaataataat aaacaacttt
    13441 ggcctatgct taagacacat aaaagtactt acatagcgag acttcaaaaa aatgcaggtg
    13501 ctagtggttt tctagttgga acacttgatg aaggtgaagc tctgataaaa gcaggttttg
    13561 aagatattat gttggcttat ccttacatgg gagaagtaaa tataaataga atcgtgacta
    13621 tggcatcaaa aataaattta atctgtgcta cagataatat agaatgtgct aaggcttata
    13681 gtgaagcttt ttctaaatca aaaatagagt gcaagctact tattattgta gattgtggct
    13741 tacaccgttt tggggttgaa ccagaaaaag tggttgaact tgctactgaa atttctaagc
    13801 tagaaaacat caagattgtt ggtataagtt cgcatccagg tcaagtgtat ggttgtagtt
    13861 ctccagaagg tgttccagat gtttgtgttc aagaagattc ttctatggag aaagcataca
    13921 aagctctaac tgataatgga tttaatatag aaattgtcgc ttctggtagt actccaacag
    13981 ttgaatctga agctgcttct aaaactataa ctgctgtaag acctggaaac tatgtatact
    14041 atgatgctat acagataggt ttaggttgtg ctactgaaga tatgtgtgct cttactgttg
    14101 tgtgcactgt aatctcaaaa aatagcagtg gatactattt aattgattgt ggaagtaagt
    14161 gccttggact tgataaaggt gcacatggaa atagtagtat tgttggttat ggtagggtat
    14221 gtggacatcc agaacttact attgattcac tttcagaaga agttggaaaa attaaagtaa
    14281 atggtgaaac tgatataaaa attggtgata gaataagaat tatacctaat cattcttgtt
    14341 ccactgcaaa cttgacaagt aatttattag gttttagagg agatacaata gaaaaagtca
    14401 tagaagtaga tattcgtgaa aattcaaaac aaatcatcct ttaaaaacac atttattctg
    14461 atattaacat gaaagtatat ttttcttaaa attattgtta ataactaatg ttaatgttgg
    14521 ataaataaaa aacataaatt ctagaaatat aaatttaaaa gaagaggctg tctcaaaata
    14581 aagataagcc tctctttcat tgttgaaatt atgaaaatat acttgatttc atacaaaaag
    14641 agtatctcat gaactaaaaa gttcactttg agatactctc tttttttaat taatatacac
    14701 attaattact atcttagttt attactatat atcaattact actcattaat ccttgtatat
    14761 attaattctt atatatcatt tatagttagg ttataatttt atgttattta cattaatccc
    14821 ataacagtga gtaaaactct tactattaac atccccacta ttgcatcaaa tataggaact
    14881 gccaacagta acattctata cttagtactg gcatttgcta caagcactat tctggcataa
    14941 tgaccaacta gagtacccat taacatacat ggcataatta atatggttgc ttgtggtgca
    15001 gtgattagac cttgtgcaaa taagtttgca gctgtagctg ctcctgctgc cttagcaaag
    15061 aatgctgcta tcaaaactac aacagattcc ccaggtagtc caaaaatacc cattactggt
    15121 ccaaaaatat ttcctaaaat atcaactaaa ccagttaatt gtaaaaactg cactattaca
    15181 taacccatta tcatggctgg taatatctgt tcaacaccta tgtaaaaacc tttttttgct
    15241 ccccccataa atatctcaac agaagttttc ttaggtttac tatttacaat tgcatttcca
    15301 gtattttgtt gactcatact acacagcctc cttagcctta ttctttttat ttcttacacc
    15361 tatgattaat ctaactagat tagcaccaag tattttacag aatatctcta taaggattat
    15421 tggacctaat gccaataatg ctattggtaa aagtggagct tgagtattta ttgtgttcag
    15481 tataacagct gaaccagcat attgatatgc tacaaatata gttctttcat catctgttat
    15541 ttccccactt tcaaataatt ctttcgtcat tattgaggct acatctgaac tagtaaatga
    15601 agatacaaat gctattcctg ctattccagg tataccaagc agaggtctaa gcaatggatt
    15661 aaatatcttt gatgccgctt tcattcctcc catagattca actacatcta taagacctac
    15721 agcaaaacag acagttggaa tcaaagtcag agcaaataag aaaccatctt ttgctcccgt
    15781 tcctccttta cccataaagt ctacaccatc tgcaatagta ccaaatgaac ctgtcagatt
    15841 agcaaaatct aatgctttaa ggaagccttg actatcttta aacacccctg aaaataatac
    15901 tatcaataca aataaagata aatacccttt ccatgttacc ttattatctc cagctgttac
    15961 atccatataa aatccccctt tatacctcaa atttatctaa gtttaaaata actgtagaaa
    16021 ttaatttcac cctctcaaac atagaatcta ctactgcata ttctcttgat gtgtggttcc
    16081 attctcctct tacaccaaat gaacagattg tgggaacgtt tgcaatagtt aaataacttg
    16141 catctgaact acctccaagt cttttagcat ttaattctcc aaatccattt tcttttgaca
    16201 cttcatttac aaatgtatga aatctcataa catcttctgt agtttcaaat ggcatcattt
    16261 cacttataaa attaacttct gtagaagttc cctctatgta agtttcttta catatctctc
    16321 taacttgttc tttaacattc tccatttctt ctactttttc aaatctaagg tctactttta
    16381 tactacaatc ttctggaata gaattttcca ctcttccacc cttaatcaca cttacactta
    16441 ctgtagttcc aacatctaaa ttagtcaatt tttgtatgcg tagtatctta ttagccattt
    16501 cttctatagc atttcttcca ccttcaaaat cattacctgc gtgtgtttct acacctttta
    16561 catgtatatt acatcctatt cttccttttc tacctacaca caaactatta tctactaatc
    16621 cagtttccat attaaaagca caaagaccac cttttgcttc cctcaatata acatctgcac
    16681 ctgttgaatc cttatgtcct atctcttcat ctccagaaaa tactattttt attggtcttt
    16741 ctttgtatcc tattttattt aatgctttaa taacatataa ggaaattatt ataccaccct
    16801 tcatatctaa aacacctggg ccatatgctt ttccttctat tattttaaat ggattctctc
    16861 caaatgtacc tgtttcaaag acagtatcca tgtgacctga aaaaattata ggctttttat
    16921 ttatattaga accaagtgta cctatcaatg tactaccatt atctccaaca tctactaatt
    16981 cgcaatctaa accttctttt tcaaattcaa gctttaatct ctcagctaac ttgtttacac
    17041 tttctttaca atgtgtatag ctttccatat taacaatctc tttccataat gaaactattt
    17101 cttctctatt ctcatcaaca aatttagaaa tttcatttaa atatgtattc ataatcttct
    17161 cctttatttt caaataaaat gtatttcttt cctaaaatct aagtttattt taggaaatct
    17221 taaaagacta gtccttaatt acaaaatagc acaattattc ttgccagtca acatattttt
    17281 cagtcttttt tatattttct gatttttctt tcaattttcc ttttgtaaaa taaattgacg
    17341 cgttttatct actattataa aatgaggtta taaattgatt gattagttac aatactctcg
    17401 aaattttaaa ggaggtttta tgaaatataa aattggtttt ttgacaacat cttattttat
    17461 gtcagataag tttaaacaag ctcttaaaga attaaatgat acttgtgaaa ttacatttat
    17521 tgagctcaaa ggggagaatc cttttaataa tttacctaat atatactatg agcaagtaga
    17581 taattttgat ggatttgtat gcagtggtat agttccatat agtgaactta taataaatat
    17641 aaaagacata aaagttccat taaatttttt aaagcttgat gaaagagatt tttataaata
    17701 cttattcaaa ttattaaata gccaaaaaga tattgacttt acaaaatcat ttatggattt
    17761 tttaagagag gacaacaatt attatgatat atattcatta atagactcta ataaatgtcc
    17821 atatacaatt aaagatttta atatccctca acctgtatat gactttaaaa aattaaatga
    17881 taaattattg gaaatacatt tagacttatt gcgagataat aagattaacc tttcttttac
    17941 tagaaattac atcattagtt gtgaacttaa taagcttgga tatgattgta tttacatggt
    18001 tccatctaca gaatcaatcc tgaatacatt taaatctctg gtgaatgaaa taacattaca
    18061 aaacttagac aaaaataaat ctgcaacttg tatagttaca gtaaatagtt cagagtacat
    18121 aaatgaagat ttgatattta aaaatgatga gatacaaaat caaatatata acactatatt
    18181 aaattctcta tcaagacatg gattctatgg agttcaggtc aaaaaaaatg atatgaaaat
    18241 agagatacac actactaaag aaatgttaaa caatatgacc aataactata cagatttttt
    18301 tatttcagaa gatttaaaag aaattaaata tagcttaaat ataggttggg gcattggaaa
    18361 tacaaatatt catgcagagc aaaatgctcg tcaagctaat ggtaaatctg cttctttgaa
    18421 tggaaactgt acttttatag taaatgatac taatgataca ataggtcctt tatattcaaa
    18481 taaaaattct aataatattg aagatagctc aatagccaat aaagttgctt cttttatacc
    18541 tctatctaat acaaatgtat ctaaaatcat gtgtatgatt aatgatagaa attcaaataa
    18601 tgtgtcagca gaaatattag cagactatat gaacataact cttagaagtg ctaatagaat
    18661 attgagtatt ttatataaag caggaattgc aactattgta aatacaaaac tcgataatca
    18721 aagaggaagg ccaaaaaaaa tatataaagt tgatttctta tcttttctaa ataagatgca
    18781 gaaattaaat tcttaatatt taatttttta ataaaagcaa tgtattttct ctaattttaa
    18841 ggaatcaaaa tgtttaaatt ttattagaat atacaacttt ttactttaaa ataccta
//
LOCUS       DAGSWI010000075        18838 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.075, whole genome shotgun sequence.
ACCESSION   DAGSWI010000075 DAGSWI010000000
VERSION     DAGSWI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.075"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            111..1397
                     /locus_tag="MJF80_002380"
     CDS             111..1397
                     /locus_tag="MJF80_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBZ0281037.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1509..1982
                     /locus_tag="MJF80_002381"
     CDS             1509..1982
                     /locus_tag="MJF80_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1641..1643,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBZ0281038.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2014..3324
                     /gene="grdB"
                     /locus_tag="MJF80_002382"
     CDS             2014..3324
                     /gene="grdB"
                     /locus_tag="MJF80_002382"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3061..3063,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBZ0281039.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3590..5122
                     /locus_tag="MJF80_002383"
     CDS             3590..5122
                     /locus_tag="MJF80_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBZ0281040.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5133..6278
                     /locus_tag="MJF80_002384"
     CDS             5133..6278
                     /locus_tag="MJF80_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBZ0281041.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDILLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6588..7667
                     /locus_tag="MJF80_002385"
     CDS             6588..7667
                     /locus_tag="MJF80_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBZ0281042.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYAKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7779..8378
                     /locus_tag="MJF80_002386"
     CDS             7779..8378
                     /locus_tag="MJF80_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281043.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SSQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8565..9437)
                     /locus_tag="MJF80_002387"
     CDS             complement(8565..9437)
                     /locus_tag="MJF80_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBZ0281044.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIDYMASIHIN"
     gene            9854..11257
                     /locus_tag="MJF80_002388"
     CDS             9854..11257
                     /locus_tag="MJF80_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281045.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            11284..12828
                     /locus_tag="MJF80_002389"
     CDS             11284..12828
                     /locus_tag="MJF80_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBZ0281046.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            12859..14250
                     /locus_tag="MJF80_002390"
     CDS             12859..14250
                     /locus_tag="MJF80_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBZ0281047.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            14443..15939
                     /gene="abfD"
                     /locus_tag="MJF80_002391"
     CDS             14443..15939
                     /gene="abfD"
                     /locus_tag="MJF80_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBZ0281048.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            16074..16349
                     /locus_tag="MJF80_002392"
     CDS             16074..16349
                     /locus_tag="MJF80_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281049.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENIEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            16373..17680
                     /locus_tag="MJF80_002393"
     CDS             16373..17680
                     /locus_tag="MJF80_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBZ0281050.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            17700..18818
                     /locus_tag="MJF80_002394"
     CDS             17700..18818
                     /locus_tag="MJF80_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBZ0281051.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
BASE COUNT         7215 a         2152 c         3732 g         5739 t
ORIGIN      
        1 ataaaaacac aataatatat ctttaaaaaa ataagataaa ttgtaaaatg atataatttt
       61 gagaaaaact caaatgcata tacacaaatt aaataacagg aggtgcaagt atgcgtcttg
      121 aattagggaa aatctttata aaagatattc aatttggtga tgtaacagaa gtaaaagatg
      181 gagtattatt catcaataag caagagatgc tacaagaaat tggtggagat gagcatatta
      241 aatctataga tatagaatta gctcgtccag gtgaaagcgt aagaataaca cctgtaaaag
      301 atgttattga gccaagagta aaagttgaag gtaatggggg aatcttccct ggtatcatgt
      361 ctaaagttga cactgtagga gaaggaaaaa ctcatgcttt aaaaggtgta gctgttgtaa
      421 ctactggaaa aatagtaggt ttccaagaag gtataataga catgactgga gaaggagcta
      481 agtatactcc attttctaaa ttaaataatg tagtagtaat agctgaacct attgatggtt
      541 taaaacaata cgctcatgaa aaagctgtta gaatgatagg attcaaagca gcaatgtact
      601 taggagaagt agcaagaaat cttactccag atgaagtttc agtatatgaa actaaacctc
      661 ttcttgaatc aataaaagaa tatccagagc ttccaaaagt aggatatgtt tacatgcttc
      721 agactcaagg tttattacat gatacttatg tatatggagt agatgcaaaa caaatagtac
      781 caactttatt atatccaaca gaattaatgg acggagcaat tgtaagtggt aactgtgttt
      841 ctgcatgtga caaaaaccca agttatgttc atataaacaa tggggttgtt gaagacttat
      901 atgcaagaca tggtaaagat ataaactttg taggtgtaat tataactaat gaaaatgttt
      961 acctagcaga taaagaaaga tcttcaaact ggacttctaa attatgtaaa tatttaggat
     1021 tagatgcagt tatagtttca caagaaggtt ttggaaatcc tgatactgac cttataatga
     1081 actgtaagaa gatagaaatg caaggtgtta aaacagttat agttacagat gaatatgctg
     1141 gacgtgatgg aggttctcaa tcacttgcag atgcagatgt aagagctgat gctgttgtaa
     1201 caggtggaaa tgccaaccaa gttgtagttc ttcctaagtt agataaagtt attggacatt
     1261 tagaagtagt agatgtaata gctggtggtt ctgatggaag tttaagagct gatggaacta
     1321 ttgaagttga aatccaagct ataacaggtg caactaatga aacaggattt ggtcatttaa
     1381 ctgctaaagg atattaagaa gtattttatt aaacgattga gtaatgttaa taaaacatat
     1441 tatagataat tataataagt gttatagata tataaaaata tattagaaat ataataagga
     1501 ggattattat gagtttactt agtaataaaa aggttcttat aataggtgac cgtgatggta
     1561 taccaggacc tgcgatagaa gaatgtgtaa aaacagtaga aggagcagag gttgttttct
     1621 catctacaga atgctttgtc tgaacagctg ctggggctat ggacttagaa aatcaaaaca
     1681 gagttaaaga tgctgctgat aaattcggag ctgaaaatgt tgtgatttta ctaggtgctg
     1741 ctgaagccga agctgcaggt cttgcagccg aaacagtaac tgctggagat ccaactttcg
     1801 ctggaccact tgctggagtt gccttaggat taagtgttta ccacgttgtt gaggaaccaa
     1861 taaaatcatt atttgatgaa agtgtatatg aagaccaaat aagtatgatg gaaatggttt
     1921 tagaagttga agaaatagaa gaagaaatgt ctggtataag agaagaattt tgtaaatttt
     1981 aatgcctaaa agcaaaaggg gtgaaactaa gagatgggaa aactaaaagc agtacattat
     2041 attaatcagt tctttgctgg tataggtgga gaagaaaaag ctgatactaa gccacatgtg
     2101 gcagaaactt tacctccaat aagtttacaa cttgataaat tattaggtga agatattgaa
     2161 atagttggaa cagtagtttg tggagatagc tatttcaatg agaatataga tagtgcaagt
     2221 gaagaagttt tatcaatggt aaaaggtttt gaaccacaac ttttcatagc tggtcctgca
     2281 tttaatgctg gtagatatgg ggttgctgct ggaacaataa ctaaagttgt aaaagatgct
     2341 ttgaatatac cagctttaac aggtatgtat gttgaaaatc caggagcaga tatgttcaaa
     2401 aaggatgttt atgtagtaga aacttctgat tctgcagcag gtatgagaaa ggcacttcct
     2461 aagatagcta aacttgcagt aaaacttgct aatggagaag aaattggaac tcctaaagat
     2521 gaaggataca tagctagagg aataagagtt aactacttcc atgaagacag aggttctaag
     2581 agagctgttg acatgttagt taagaaaata aaaggtgaac catttgaaac tgaataccca
     2641 atgccaaact ttgatagagt tgacccaagt aaagctgtaa aagatttatc aaaatgtaaa
     2701 atagctttag ttacttctgg tggtatagtt ccaaaaggaa atccagatag aatagagtct
     2761 tcttcagctt ctaaatatgg aacttattct atagcaggag ttatggattt aacagaagag
     2821 acttatgaaa ctgcacatgg tggatatgac ccagtgtatg caaatttaga tgcagataga
     2881 gtattacctg ttgatgttct tagagattta gaaaaagaag gtgtaattgg aaaattacat
     2941 gagactttct atacaactgt aggtaatggt acatctgttg caaactctaa aaaatatgct
     3001 tctgaaattg gagcagcatt agtggctgat ggtgtagatg cagtaatatt aacttctaca
     3061 tgaggtactt gtactcgttg cggtgcaacg atggtaaaag aaatagaaaa aactggactt
     3121 ccagtagttc atatgtgtac agtagtacct atttcattaa cagtaggggc taatagaata
     3181 gttccaacta tagcaatacc tcatccactt ggaaatccag cgcttgaccc tacagaagaa
     3241 aaagccctaa gaagaggcct tgtagaaaaa gcattaaatg ctttaactac agaagtagat
     3301 ggtcaaacag tatttgaaaa ataaaaataa cacacacata tattatatag ataaaaatat
     3361 aaacacacac taaaaataaa aaataactat acacaaaaaa aataatatag atttcatgaa
     3421 tggataaatt ggagttattc attcatgaaa tcttaaaaaa tgccaaaata cataaacaat
     3481 aatagaatca aatatacaat aaaatacaat gattagagct tttaataagt ttaataagat
     3541 taataagatt aaaaaataag tgttcataaa atttatggag gtgcacaaaa tgacttatcc
     3601 agttttaaaa ggggcaggct atgtacttat tcatactcca gatatgatag tgcaaaatgg
     3661 aagtacttgt acagttgaaa gagcaacaaa tccagattca gaatttttaa aagaagtaag
     3721 taatcatata agaagttatg aagatgtagt taactacatg ccaaatcaag tttatatagg
     3781 aaatagaaga ccagaagaat taagagattt accaatgcca tggtgtgaac aaaagattga
     3841 gggaactaga aatggtaagt ttggagaaat aatgcctcaa gatgagttca tagctttaat
     3901 gcaaataagt gatgcatttg atttagtaaa attatcacaa gaatttattg atgaagtaaa
     3961 gccaaaaatc gaaaataact accctgaaat tgcaccattt gtaggtaaat taaagggtga
     4021 tgatatagaa gaaggtaaag aattagtagc aacacacata gctgaaggat tataccatga
     4081 tggaaaattt gttggttatg taaaaagagc acatgatgta gatgttaact taaatgccca
     4141 tacaatgttt gaaaatctag tagtaaaagc atctggagtt ttatctgcta ttcaaatgct
     4201 aagacatagc aaaatagacc ctgctgaaat agattatgtt attgagtgtt cagaagaagc
     4261 ttgtggagat ataaaccaaa gaggtggagg aaactttgct aaatcaatag ctgagatagc
     4321 aggacttcaa aatgcaactg gttctgacac aagaggattt tgtgcagcac caactcatgc
     4381 attaattcaa gcagcagctt tagtaaaagc tggtatacat aaaaatgtaa tggtagtagc
     4441 aggtggagca agtgctaaac ttggaatgaa tgctaaagac catgttaaaa aaggtcttcc
     4501 tgtacttgaa gatgttgttg gaggatttgc agtacttgta tctgaaaatg atggtgtaaa
     4561 tccagttata agaactgatt taacaggtaa gcatactgtt ggaactggtt cttcaccaca
     4621 agcagttatg acagcactta taacttcagg tcttgataga gctaatttaa aaataacaga
     4681 tgttgatgtg tattcagttg aaatgcaaaa cccagatata actaagccag caggagcagg
     4741 agatgttcca gaagcaaact acaaaatgat aggggcactt gctgttaaac gtggagactt
     4801 agaaaagaaa gaattgaaag attttgtttc taacaaagga ttaccaggat gggcaccaac
     4861 tcaaggacat ataccatcag gagctcctta tataggattt ttaatagatg atttaactac
     4921 aggaaataga aatagagcta tgatagttgg aaaaggtagt ttattcttag gaagaatgac
     4981 taacttattt gatggagtat cttttatagc tgaaagaaat acaggtgtta ctgaagagac
     5041 ttcaggaata tctaaggatg aaattaagaa gataatagct gaatctatga aaaaactagc
     5101 attagatatg ttagaagaat aggggtgagg atatgtcaaa aaaagtaatc gcagatgtat
     5161 tcttagaagt agctaatgca atagaaagcg gcgaatttgg caaaaaagta aaaattggtg
     5221 ttactacact tggaagtgaa catggtgtag aaaacatggt taatggtgca caattagcaa
     5281 aatctaattt atttgatatt ctgcttattg gaccaaaagt ggaaactgac cttgaagtag
     5341 ttgaagttaa tgatgaaaaa gaaatgcatg caaaaatgga agaattatta gattcaggat
     5401 atattgatgc ttgtgttact atgcattata attttccaat aggtgtatct acagtaggta
     5461 gagtaataac tccagcaaaa ggaaaagaaa tgatacttgc aacaacaaca ggaacaagtg
     5521 ctactaatag aatagaggca atggtaagaa atgccattta tggtattgct acagctaaat
     5581 caatgggtaa caaatgccca aaagttggaa tacttaatgt tgatggagca agacaagttg
     5641 agaagtgctt aaaagaatta aaagataatg gatatgacat ggagtttgcg gattcaatta
     5701 gagcagatgg tggatgtgta atgagaggta atgatttact tgtaggtgca cctgatgtta
     5761 tggttactga tacattatct ggaaatatat ttatgaaagt attttcttca tatactacag
     5821 gtggagatta tgaagctcaa ggctttggat acggacctgg agttggagaa gactatgata
     5881 gaaaggtact tatagtatca agagcatcag gttctccggt agtagcaaat gctttaaaat
     5941 acgcttatga tgttgtaaag ggagatataa gtaatgttgc aagaaatgaa tttgcaaagg
     6001 ttaaaaaagc aaaatttgat gacattatat cctctcttac taaaaaagaa gttaaagctg
     6061 aaaaagtaga agttaaaatg ccagataagg aaattgttac tcgtcaaata gctggtgttg
     6121 atataatgga tttagaagat gcagtatctg agttatggaa aaacggaatc tatgctgaaa
     6181 gtggtatggg ttgtacaggt ccaatagttt tagtaaatga agctaaagga gacttagcag
     6241 ttgaaacttt agttaaagct ggatatactg caaaataagt acatttttta tatgtaaata
     6301 agaacatata gttataattt tattgattat aattatatgt tctttttatt ttatatcttg
     6361 tcaatattta tatattaaca caatccattt atttgattct ctagctaatt tattatattt
     6421 attcaattaa aattaagctt tctaactaaa taaaaactaa tgaagtatta aaatgaaatt
     6481 gataaaatat gaaaaaattt taggaataaa gtatctgtta gataaaaata agattaatta
     6541 gtgatataat ataattaaga taaaatttat tatttggagg taaaatcatg agcagagtaa
     6601 aaaatgtagt agaattgttg gaaactaaag gtgttgatgc actatatcta acaaagaaaa
     6661 ctaatgtaaa ctatataagt ggttttcctg atgaagaagc atatgcagtg atttgcaaag
     6721 atggaaactt tttagttact gatagtagat atatggagct tgcagaaaag gtttgcaaag
     6781 attttgaaat tataaattgg cataattttg atagaagtgt ggcaaaggca gttaaaagtg
     6841 tatgtgataa agtgggtata aagaaacttg ggtttgaaag aactaatata gtgtttgata
     6901 aatatgaaga acttaaaaat ctgattgaga aggataatgg agaattaata cctacagaaa
     6961 atattgtaga gactttgaga tatgcaaaag acaaagatga aatcaaaaat acaagaaaag
     7021 cttgtgaaat agcggataag gcattagaag aattgattcc acatataaaa gctggagtta
     7081 gcgaaattga actggctaca aagttagagt actttatgaa aatgaacgga gcacaaaata
     7141 tagggtttga aactatactg atatcaggag caaagacttc attgcttcat ggtaagccta
     7201 gtgataagat tatagaaaaa ggtgattttg tacttataga ctatggtgct atgtataatg
     7261 gctatatatc tgatacaact agaactttta tagttggagg agcttctgaa aagcaattag
     7321 aaatatataa tttagttaag gaagctcaaa atgtaggggt tgagaacatg aaagctggtg
     7381 tacatgcgac aattccagat gctgagataa gaaaagttgt caaaaaatat gaagactatt
     7441 actatcaagg tataggacat ggagtcggta gagatgttca tgaagaacct ttcattggta
     7501 attatggtga taaaatcata gaagagggat gtataataac aatggaacca ggtatttatt
     7561 tccctggatg gggtggagtt agaatagaag atactgtttt gattactaaa aatggtccag
     7621 aaagacttac taaattccct aaagatttga tgattttaga caaataattt aatttatata
     7681 tattttttac ataaaatata acataatctc actattaagc tctatttaca tattatagta
     7741 aagagggctt aattttattg tgagaggtga tagtatatat gtataaaaaa cataaattta
     7801 taaaaaatat aaaaaaagcc tggtataaaa tcaaattaat tgatagatgc cttataattg
     7861 taatggcaat attaatgttt caatctatat ataatttatt tgttaatgaa gtaaattcac
     7921 aagatactac aactattgat gtagtagtaa gaaccacttc agcagctata tttgggtatt
     7981 ttttaagtgc taattttata aagagacctt taaggaagta tacaaatact gatgtatctt
     8041 caaatctttt tatagataat aatttatccc caaaaggtca agagtctagt caaaataata
     8101 taatgaatgt caaaaatacc attggattta cttcagaagc tgataattat gaaaagaaaa
     8161 tacctattaa taataatgaa ggttttgaag aaggagaaac tagtgaattg caaattatta
     8221 tagctactgt aatttgtata gtagcattaa cagtactttt tgttgtaaga aattttacaa
     8281 ctacaacatc agcttcttta ggtactattt cacagatgag agattttgtc tcaggatgcg
     8341 tgggtttttt gctaggttgt ccaagtaaac ctgattaaaa aagtattatc aataaaaggt
     8401 attttaaaaa gtatcagtat aataatattt agatttaaat gtatacaatt ataattctat
     8461 aacattctat attgtatttt tatactgata ctctaaacta tactatttat taaaatattt
     8521 taacattgta tttaaacaaa atattatatc tgtataatat aaatttagtt tatatgaata
     8581 gatgccatat aatcaataaa aagtttcata ctatgagata tgtattgatt tcttctccaa
     8641 agaaccccaa tatttgcagt aattgcacgt tctaatggta ttgaacagag attttctgta
     8701 ttttgaaata tttctttata caatattgtt gaagccaagt cattttctag taaataacgt
     8761 atggtagaaa gttgatttga gtgtaaaatt atatttggct tagctttttc aagtcttttt
     8821 gtaataatct tatagtgata agaaccttca gataagatta caaaaggtac attttttaac
     8881 atataattcg atataatggg atgttttgcc aattcatttt tgggatttgt aactaagtat
     8941 aattctgttt tatagattgt ctttgaatct atatcatcat aaatatcact gtcaattata
     9001 ccaatacaaa aatcaagtgt agattcttca atcattttag ctccaacaaa tgttggaact
     9061 tcaaaaattt taagctctat atctggataa attacattaa atctaggaag tatttttgaa
     9121 agcaaaaacg tacctaaaac tgtaggaata cctattttga gagaagcttt tctctttttt
     9181 cctaaatcaa gtgcgtcaca ttgaaaatcc tcaaattgtt ttataaaatg ttttgacttg
     9241 tctaaaaaaa tttttccttc aggcgttaac tctattttat tacctattct gtaaaaaagt
     9301 tcaaagccaa gttctttttc taattcttta attgctaatg ttatggatgg ttgagaaata
     9361 tgaatacttt ctgcagcctt agtcatgttt ttatatttac agacagtttg aaaatagtat
     9421 aaatagttta tattcatttg gtattagttc ctttcattta catattagtg acaagtatat
     9481 aattaacaat agcataagtt ttgtatattg ttgtaaaaaa atagaatagt tttaaactat
     9541 catagcataa gtttttgata ttttcaatct attaagaata gtgctattat taaattaaca
     9601 aaagagaata gctggtatgg tttagagtaa atcaatctat attacagtta tatcaacgaa
     9661 tatcaaaaaa ttatctatta gaaaataaaa attattttta caattaaatt gttaattaaa
     9721 tcataaagaa aaaagtgata ttaacaagat aaaatttttt taatcagaaa tgttaattta
     9781 ataacaaaat agagtattgg ggagaaagga gaaatatatt ttttaataat atttgttaat
     9841 ttaataacga aaaatgaaag ggagtgattt agtgaagttt gaattgatgg attttttaat
     9901 gaatcctttt gtccttatgt ttgctgcagt aattacagga attttatttg gaaaaattaa
     9961 atttgggaag tttaattttg gggtatcagg agcattattt acaggattat ttattggatg
    10021 gcttgcatat agcttgggga atctaataat agcaaaaggg gaaacagcag caggatataa
    10081 ggcagcaact gtaatgatgg ggaatggaat tatttcttct gacttctttg atttcttttt
    10141 aataatattt gttgcagcag ttggtctatt ggcagcaaaa gatatgaaag ctgtattaaa
    10201 gaagtatgga gcaagatttg taattttagg agttcttata acttttatag gtggctctat
    10261 gacttatgca atgactttac tatcaagtga taaaggaagt agtgcttatg aagtttctgg
    10321 cgtttacaca ggagcattaa caagttcgcc tggattagct gctgctttag aaacagctgg
    10381 taaacatgca gaagatgtat caaaagaatt tgaaaaagct tcaataaaag ataaaaaaga
    10441 aattttaaaa gtagtcgacc cagaaggcaa attggatgtg aatacaacta catcactgac
    10501 tcaagagcaa atagataagt atattgcata tgctgaagct ggtgttggta taggtcatgc
    10561 agtcgcatat ccttttggag ttttaatagt aatcttaggt gtaaatttcc ttccaaaatt
    10621 atttaggatg gacctaaaag aagaaagaag aaaatatgag aaggaaatga aggaagcaag
    10681 agatagtgtt tctggtaaaa cgataccaga agttccattt aatataataa catttttctt
    10741 gacatgttta gctggatact tagttggagg aatacatgta tttatgggac cactagggta
    10801 cttcacacta ggcgcaacag gaggttcact aattgtatct ctagttcttg gatatatagg
    10861 taagataggg gtagtaaatt tcagaatgga agaaaaggtt ttaaatattt taaaacaaat
    10921 agggctagta tttttcttag caatagttgg attaagatat ggtggaaaag tagttgattc
    10981 aattatgaca tctggtatgc acctagcctt agtagcaata gcagtaggtg ttacagctat
    11041 gatgatagga ttcttagttg gcaaatacgt atttaaatta aattggattt tattatctgg
    11101 agctgtctgt ggtggtatga catcaacacc aggtcttgga gctgctgttg atgctcttga
    11161 tagtgatgac ccagcagcag gttatggtgc tacgtatcca tttgcattat taactaaagt
    11221 tatattggtt atagttttac acaaacttcc aatgtaaata agattttata tggggggata
    11281 tttatgaata atgaaggagc atttgaagaa ttaagtggaa gattaagaaa taaagaattg
    11341 tgtgatttaa taatgaatgc agatgaagct gctaaaatta tagaggatgg catggttatt
    11401 ggagttagtg gatttacacc ttcaggctat ccaaaagcag taccattagc agtttcagaa
    11461 agagctaaaa gtggagaaaa gataaaatta accgtatatt caggagcatc tttaggccca
    11521 gaagttgatg gagcatggtc agaagcaggt ataattgaaa gaagacttcc ttatcaaact
    11581 aattcaatac ttagaaataa tattaataaa ggtgttgttg attatataga tatgcaccta
    11641 agtcattcta ctcaattttt aaattatgga actataccta aagtagatgt agctatagta
    11701 gaagcacttg caataactga agaagggaat attataccaa ctagtgggat aggtaattct
    11761 ccatctttta taaaaagtgc agataaagta atagtagaga taaatcttgc aaaaccaatg
    11821 gaaatggaag gtatggcaga tatatatatt acggaaaatc caccaaatag aaaaccaata
    11881 gaaataaatc atcctaagga tagaattgga acaacatata taccttgtgg tttggataaa
    11941 attgcaggag tagtaattac caatatgcaa gataagaccc gtccattggg ggttgtagat
    12001 gaagcttcga aaaaaatatc aaataatatt attgcttttt taagagaaga agtaaaggca
    12061 ggcagattat caaagaattt acttcctctt caatctggtg taggaagtgt agcaaatgca
    12121 gtattatatg gattatgtga atctgaattt gaaaatttaa cttgttatac agaggttgta
    12181 caggactcta tgttggattt aataagaatg ggtaaagtta caatggcatc tactacatct
    12241 gtaagtccat ctccagaagg attaataaaa tttgaaaaag atattgattt ttttaaggat
    12301 aagattatat taagacctca agaaattagt aacaatccag aaatagccag aagaattggt
    12361 gtaattgcaa tgaacactgc aattgaagtt gatatttatg gaaatgtaaa ttctactcac
    12421 ataatggggt caaagatgat gaatggaatt ggtggttctg gtgattttgc tagaaatgga
    12481 gcaataacta tatttagtac tgagtcgatt gcaaaaaatg gtgatatatc atcaatagta
    12541 cctatggtat ctcacgtaga ccacactgaa catgatgtaa tggtaattgt aacagaacaa
    12601 ggatatgctg atttaagagg tcttgcacct agagaaagag ctataaaaat aatagaaaat
    12661 tgtgcccatc cagattataa agaacaactt agggattatt taaatagagc ttgtaaaagt
    12721 ggagcaaaac aaacacctca catattggac gaagctttat catggcatag taaatttatg
    12781 agtacaggta caatgaaaaa ggctgaaact tttaaaagtg cattataaaa tatttaataa
    12841 attggaggta gattttaaat ggaaaaagca gtggaaaact ttgaagattt aagtaaggaa
    12901 tacattaatg gttatattga gagagcaaga aaagctcaaa gagagtttga atgttatact
    12961 caagaacaag tagataaaat agtaaaaata gttggaaaag tagtgtatta taatgctgaa
    13021 tatttagcta aacttgctgt agaagagact ggaatgggag tatatgaaga taaggtagct
    13081 aaaaataaaa gcaaagcaaa agttatatat aacaatttaa aagataaaaa atcagttggt
    13141 ataattgata tagatagaga aactggaata acaaaggtag ctaaaccagt tggagtagta
    13201 gcggcaataa ctccttgtac aaatccaatc gttacaccta tgagcaatgc aatgtttgca
    13261 cttaagggaa gaaatgcaat tattatcaca cctcatcata aagcaattgg atgtagtaca
    13321 aaaactgtag agatgattaa tgaagaatta gaaaaaatag gagctccaga aaatttaata
    13381 caaatacttg accaacaatc aagagaaaat acaagaaatt taatttcatc agcagatgta
    13441 gtaattgcca ctggaggaat gggaatggta aaagctgcgt actcaagtgg aaaaccagca
    13501 cttggtgtag gggcaggaaa tgttcaatgt atcatagata gagatgtaga cattaaagaa
    13561 gcagtaccta aaatcatagc aggaagaatt tttgataatg gtatcatttg ttcaggtgaa
    13621 cagtcagtaa tagtagcaga agaaatgttt gataaaataa tggatgaatt taaaaataat
    13681 aaaggtttta tagttagaga taaagtacaa aaagaagctt tcagaaatgc tatgtttgta
    13741 aataaatcta tgaataaaga tgcagtaggt caatcagttc atacaattgc aaaaattgca
    13801 ggtgttgaga taccagaaga tacaaaaata atagtaatag aagctgatgg accaggagaa
    13861 gaggatatta ttgcaaaaga aaaaatgtgt ccagttatat ctgcatacaa gtataaaagt
    13921 tttgaagaag gtgtagctat tgcaaaagca aatttaaatg tagaaggaaa aggacatagt
    13981 gtatctatac attcaaatac agttaaaaat atagaatatg caggagaaaa tatagaagta
    14041 tcaagatttg ttattaatca atgttgtgct actagtgctg gaggaagttt ctttaatggt
    14101 cttgcaccta ctaatactct aggttgtggt tcttggggaa ataacagcat ctctgaaaat
    14161 cttgattata agcatctaat taatatttct agaattgctt attatatgcc agaaaatgaa
    14221 gtccctacag atgaagaact ttggggataa aatttagaca aaaaatattc attctaaatt
    14281 aaagtaggaa tatattttaa tttagaatga atttaagaca taaattaaat ttaaattagt
    14341 ttattggact aaatttaaaa tattaagaga catatagaag aaggaagtat tataatttaa
    14401 aataaataaa aaaatctata aaagagaagg agtggtttta ttatggcatt aatgacagga
    14461 gctcaatata ttgaaagttt gagaaaatta aatactaagg tttatatgtt tggtgaagag
    14521 gttaagaatt gggttgacca tcctatgatt agaccatcta taaattgtgt agcagcaact
    14581 tatgacttag ctcatgaccc agaatatgca gatttaatga cagttacttc aaatatcaca
    14641 ggtgaaaaaa taaatagatt tggtcatctt catcaaagtg ttgatgattt gataaaaaaa
    14701 gtaaaaatgc aaagactatg tggtcaaaaa acagcttctt gtttccaaag atgtgttggt
    14761 atggatgcgt ttaatgcagt gtattcaaca acatttgagt gtgataaagc ccatggaact
    14821 aattatcatg ataattttgt aaaatactta acttatatac aagaaaacga tttagtagtt
    14881 gatggagcta tgactgaccc taagggagat agaagtctat cacctagtgc tcaacctgac
    14941 ccagatatgt tccttcatat agtagaaaga agagaagatg ggataattgt aagaggagca
    15001 aaggcacatc aaactggttc aataaattca catgagcacc ttataatgcc tactataagt
    15061 atgactgaag cagataaaga ctacgctgta tcatttgcag ttccttcaga tgctgaagga
    15121 gtgtttatga tttatggaag acaatcttgt gacactagaa aattagaaga aggtgcagat
    15181 gttgatttag gaaataagga gtttggaggt caagaagctt tagtagtatt tgataatgta
    15241 tttgttccaa atgatagaat tttcttatgt ggagaatggg atttctctgg tatgttagta
    15301 gagagatttg caggatacca tagacaaagt tatggtggat gtaaagtagg agtaggtgat
    15361 gtaataatag gagcagcagc tttagcagct gattataatg gagcaaacaa agcttcacat
    15421 ataaaagata aactaataga aatgactcac cttaatgaaa gcttatattg ctgtggtata
    15481 gcatgttcat cagaaggtca caaaacagaa gctggaaact atcaaataga tttacttctt
    15541 gcaaatgtgt gtaaacaaaa tgtaactaga tttccttatg aaatagtaag acttgcagaa
    15601 gatatagctg gagggcttat ggttactatg ccttcagaaa aagactttaa atcagacctt
    15661 aaagttggaa caagtggaat gactattggc gaagtatgta acaagtattt taaagcttct
    15721 tcagtggctt caacagaaga aagaatgaga atattaagat tcttagaaaa tatatgcctt
    15781 ggttcatctg ctgttggtta tagaactgag tcaatgcatg gggcaggttc accacaagct
    15841 caaagaataa tgatttcaag acaaggaaac ataaatcaaa agaaagaatt agctaagaaa
    15901 attgcgggaa taaaaaaaga ggaagcttta aatttataaa aagttgatat ataaagggaa
    15961 attatatatt aatgtgtatt aattaatgaa aaaatatgta gtataagaga tagttataaa
    16021 attatggtgg tttttagcta agtaaagaaa tttttaataa ggagaattta cacatgacct
    16081 gtggagttag attttgtggg ggctgtaacc ctagatacga aagagggaag gctcttacaa
    16141 taatcaaaga gcacttcaat ggtagggtag attttagtat agcacaagag aacatcgagt
    16201 atgatttgct acttataatt ggaggatgcg caactctatg cgcatcctat caacaatata
    16261 aagtgaaaaa tgaatgtgtt aagatgtggg attttagcca tatagaaaga gttataaatg
    16321 tgattgaatc agcaatttgt gaaacttaaa aaagctaaat ggggggattt cattgagttg
    16381 gcaagaattg tatcaaagta aattatgtag tgctacagaa gctgtaaaac agattaaaaa
    16441 cggtgatact gtagtatttg cacattgtgt aggtgaacct cctgcactag tagaggctat
    16501 gattgaaaat gctgaacaat ataaagatgt tgagattaaa catatggtaa gcttgggtag
    16561 tggtggatat actgctaagg gaatggaagc tcattttaga gtaaatccaa tgtttgtaag
    16621 tggaaatgta agaaaggcaa ttgaaaatgg agatggtgat tttacacctg cattcttcca
    16681 tgaagtacca aagttgctta gagaaaaaag attaaaatgt gatgttgttt tagcacaggt
    16741 aactccacca gatgaacatg ggtattgtag cttaggaaca tcagttgatt atacttatga
    16801 agccattaaa actgctagaa cagtaattgt tcaagtgaat gaccagtttc ctagaaccta
    16861 tggtgaggta gtccatgtat cagagtttga ctatatagtg gaaaaatcac aacctttatt
    16921 tgagctacaa cctgcaaaga ttggagaagt agaagaagca attggtaaaa attgtgcttc
    16981 attaattgaa gatggttcta ctttacagct agggattggt gggataccag atgcagttat
    17041 gttattttta actgataaaa aggatttagg gattcattca gaaatgattt cagatgggac
    17101 tttggccctt tatgaaaaag gtgttataaa tggtaaatat aaaaattttg ataaagaaaa
    17161 aatgacagtt accttcttaa tgggtactaa aaaattatat gactttgcaa ataataaccc
    17221 tgcagtagag gtaaaaccag tagactatgt gaatcatcca gcaattataa tgaaacaaca
    17281 taagatggtt tctataaatt ctgctataca agttgattta atgggacagg tagttgcaga
    17341 ggctatggga cttagacaat ttagtggtgt tggaggtcaa gttgacttta tacgtggagt
    17401 gtctatgggt gaagatggaa aggcaattat agctatgcct tcaataacta caaaaaaaga
    17461 tggtacagta atttcaaaaa tcgtatctat tgtggatgaa ggtgcaccta taacgacatc
    17521 aagaaatgat gttgactata ttgtaacaga atacggtata gcagaattaa aaggaaaatc
    17581 tcttagagaa agagcaagaa atttaataaa tatagctcat ccaagtgtaa gagaacatct
    17641 agcagtagaa tttgaaaaga gatttaagga gaaatattaa taatagaggg gtgttaataa
    17701 tggaagcact tagagtagtt ccaaagatat tttactttga tacatttaaa gaatttaatg
    17761 aagaatttaa aataggaaaa aatgatttag taattactaa tgagtttata tatgagccat
    17821 atatgaaacc tcttggaata gatacaaatt taatttttca agaaaagttt ggaacaggtg
    17881 aaccatctga tgaaatgata gactccatga caaaggaaat gaagaaatac aattttgata
    17941 gaattattgc atttggagga ggaacaatag ttgatatatg taagatatta gcacttgatg
    18001 ttccagaaaa gtctattgat ttatttgaag gagaagtatc acctaaaaaa gtaaaagagt
    18061 tagtagtagt tcctacaaca tgtggtacag gtagtgaagt aacaaatgta gctattgcag
    18121 agttaaaatc taaacatact aaaaaggggc ttgcagtaga agaaacatat gcagactatg
    18181 cagtgttaat tccagagaca gtaaaagggt taccttacaa attctttgtt acaagttcgg
    18241 tagatgctct tatacatgct attgagtctt atctttctcc aaaagcaagt ccatttacag
    18301 agatgtattc tctacaagca ataaaaatga ttatggatgg gtataagaag atagttgata
    18361 aaggagaaga agagagattt aatcatctta gagactttgt acttgcatca aattatgcag
    18421 gaattgcttt tggaaatgct ggttgtgcag cagttcatgc actatcttat tcaataggtg
    18481 gagctttcca tgttgcacat ggtgaagcaa attatcagtt ctttactgaa gtatttaaga
    18541 tgtattctag aaaaaaacct aatggaaaaa ttataaaatg tacaaaaatt ttagcagatg
    18601 cccttgaatg tgaccctaac tgtgatgttt atggagaatt agaaaagttt ttaaataaat
    18661 taatagctaa aaaagctttg agagaatatg gaatggtgga aagtcagata gatgaattta
    18721 cagatagtac aatagcaaat caacaacgac tacttgctaa taattatgta gagcttagta
    18781 gagaggaaat aagagagata tttgcaaatc tttattaata caatttttta tttatatg
//
LOCUS       DAGSWI010000076        18819 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.076, whole genome shotgun sequence.
ACCESSION   DAGSWI010000076 DAGSWI010000000
VERSION     DAGSWI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18819
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.076"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            404..1453
                     /locus_tag="MJF80_002395"
     CDS             404..1453
                     /locus_tag="MJF80_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBZ0281052.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSVNQNGDSTNNNNQQNNANNIQQDNTNDNQQNNNS
                     NVPTANSNAVQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSK
                     AQSKYGQTVSKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSIN
                     SSYYTARFVTAKRIL"
     gene            1749..2669
                     /gene="pyrB"
                     /locus_tag="MJF80_002396"
     CDS             1749..2669
                     /gene="pyrB"
                     /locus_tag="MJF80_002396"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBZ0281053.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            2708..3400
                     /locus_tag="MJF80_002397"
     CDS             2708..3400
                     /locus_tag="MJF80_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBZ0281054.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            3393..4295
                     /locus_tag="MJF80_002398"
     CDS             3393..4295
                     /locus_tag="MJF80_002398"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBZ0281055.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKSRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            4380..4964
                     /locus_tag="MJF80_002399"
     CDS             4380..4964
                     /locus_tag="MJF80_002399"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBZ0281056.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            5179..6105
                     /locus_tag="MJF80_002400"
     CDS             5179..6105
                     /locus_tag="MJF80_002400"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBZ0281057.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITKVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(6157..7047)
                     /locus_tag="MJF80_002401"
     CDS             complement(6157..7047)
                     /locus_tag="MJF80_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBZ0281058.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            7235..8266
                     /locus_tag="MJF80_002402"
     CDS             7235..8266
                     /locus_tag="MJF80_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBZ0281059.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGTVQVVS
                     AVVLIKIFI"
     gene            8560..9435
                     /locus_tag="MJF80_002403"
     CDS             8560..9435
                     /locus_tag="MJF80_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBZ0281060.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYAEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            9453..10943
                     /gene="cls"
                     /locus_tag="MJF80_002404"
     CDS             9453..10943
                     /gene="cls"
                     /locus_tag="MJF80_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBZ0281061.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            11136..11420
                     /locus_tag="MJF80_002405"
     CDS             11136..11420
                     /locus_tag="MJF80_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBZ0281062.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            11453..13081
                     /gene="groL"
                     /locus_tag="MJF80_002406"
     CDS             11453..13081
                     /gene="groL"
                     /locus_tag="MJF80_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBZ0281063.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            13343..14083
                     /gene="larB"
                     /locus_tag="MJF80_002407"
     CDS             13343..14083
                     /gene="larB"
                     /locus_tag="MJF80_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBZ0281064.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            14167..14901
                     /gene="larC"
                     /locus_tag="MJF80_002408"
     CDS             14167..14901
                     /gene="larC"
                     /locus_tag="MJF80_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBZ0281065.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            14931..15350
                     /locus_tag="MJF80_002409"
     CDS             14931..15350
                     /locus_tag="MJF80_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBZ0281066.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILDRKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      15410..15511
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15732..17267
                     /gene="guaA"
                     /locus_tag="MJF80_002410"
     CDS             15732..17267
                     /gene="guaA"
                     /locus_tag="MJF80_002410"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBZ0281067.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(17344..18390)
                     /locus_tag="MJF80_002411"
     CDS             complement(17344..18390)
                     /locus_tag="MJF80_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBZ0281068.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(18467..18682)
                     /locus_tag="MJF80_002412"
     CDS             complement(18467..18682)
                     /locus_tag="MJF80_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0281069.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
BASE COUNT         7364 a         1983 c         3453 g         6019 t
ORIGIN      
        1 attaggaaat aggctttgat ttatgtaggg ttaaaaattt cacagaatat ctaactttat
       61 actaaatcca tcaaaaaata tacgccatat aaaagcgcat aaaaaaacac ataaaacaat
      121 atttatgcgc actttaacct cgaatggtac aaaattcttt acttttacag acccatatta
      181 tataattaag taaataatgt atatttaggt gacatattgg ataagctaat ttatttattt
      241 tattaatata ataatactat aaaagttata taaagtggtt tacaaaaaag ttacagatag
      301 agcttttatt aatgtattgt gtattagtat tttatcctta ggtacacttc acctacaaaa
      361 tagaatcata agataattat gggaaaattt taggaggttt atcgtgaaga aaaagatatt
      421 aataccagta tttgcatcag ttatggcatt atcagtaagt tcaattgtaa atgcagatga
      481 agtaaatgat tcatctcaaa ataaggatga taaaacaaat actgaattaa atttaggtga
      541 atataaagaa gtaaaatata aagttgcaaa aataaaagat ggagtagcta taaagataag
      601 agaagaaggt caagttcaaa acatagctta ttctggagat gaatttacgg ttttaggaac
      661 tcaaggagaa tgggttaagg ttaaagttga agatggcgaa ggttggttag ctactagata
      721 tgtagatata tctgaaggtg taggatatac taacgctgat aaagtaaact taagaaaaga
      781 taagagtgaa agttcagaag taattgaaga gctggaaaaa ggaagttctt tattggtttt
      841 ggaagataat ggagattggt taaaagttaa agatggggaa acagaaggat atgtaaaatc
      901 atcttatata tctgataaag ctcctgtaat agaagagcca caagtaaacc caagtgttaa
      961 tcaaaatgga gattctacaa ataataataa tcaacaaaac aatgcaaata atattcaaca
     1021 agataataca aatgacaatc aacaaaacaa taattcaaat gtgccaacag ctaactctaa
     1081 tgcagttcaa gctgtactta acttggcata ttctaagcaa ggttgtccat atgtttgggg
     1141 agcagaagga ccaaacactt ttgactgttc tggatttaca caatatgtgt atagaaatgc
     1201 agtaggaaag aatataccaa gaacatctaa agcacaatct aaatatggac aaactgtaag
     1261 taaagcaaat ttacaaccag gagacttagt attcttcaca actaatggtt ctggttctgt
     1321 tagtcatgtt ggaatatatg taggtggggg taatatgata cattcaccat caacaggaaa
     1381 aacagttagt gtaactagta taaattcatc atactataca gcaagatttg ttacagcaaa
     1441 aagaatatta taatcggttt gtaatatgac aaattgataa atacataaat atatataaac
     1501 gctctatatt atatttaatt taatcatgga aacaaaaaat gattatattt taaatgtact
     1561 atagagcgtt tttgtatcac tttaaatcta atcaagattc aagttaatat gactgttaaa
     1621 taggagcctt ttcatatgat gattatttgc gcaatccctt gacaggatag ggcaaaggtg
     1681 gtaagctact aacagtacaa aaatactttt aattttagtt cagagaggct aaaaaaggag
     1741 gacgaaagat gttaaaatca agaaatttaa tccaaccaga agacttttca atagaagaaa
     1801 tagatgaaat attagaatta gctcaaaaaa taattgacaa tccgtcaaaa tattctcgta
     1861 tatgtgaagg aaaactatta gctacattat tttatgaacc atcaacaaga acaagattaa
     1921 gctttgaatc tgctatgaac agattaggag gacgtgtagt aggcttttca gagccaaatt
     1981 catcctctgc atcaaaaggt gaaactctag gagatacaat gaggatagta tctggttatg
     2041 ttgatataat agctatgaga catcctcaat caggtgcagc ttcagaagca gctagatata
     2101 cggaagtgcc atttataaat gcaggagacg gcaagaacca gcatccaact caaacactta
     2161 cagacttact tactataaaa tcattaaaag gaactttaga aagccataca ataggattat
     2221 gtggtgattt aaaatatgga agaactgtgc attctttggt taaggctatg gcaagatata
     2281 aaaatacaaa gtttgtattt atagcgcctg aagagttaaa aatgccagat tatataaaag
     2341 aagctataaa gggacatgca tattatgaaa ctaataattt ggatgatgtt ataggaagct
     2401 tggatgtttt atatatgact agagttcaac aagaaagatt tgaagataaa tcagagtatg
     2461 aaagacttaa aaactattat attttaaata aagctaaatt agaaaaggca tctaaagata
     2521 tgttagtaat gcatccatta ccaagagtaa atgaaataga catagatgta gatagtgatg
     2581 atagagctgt atattttaaa caagctaaat atggaatgta tgttagaatg gctttaataa
     2641 taaaactttt aggtataaat gaagactaat agttatcata taatagtaaa acaaaggaga
     2701 ttttgggatg tataaaattc tggaaaatat atatataggc gaagatatgt atagaatgaa
     2761 agtaaaaggt aattttgaag gtaaaatggg acaattctat atgttaagag catgggatac
     2821 ttatccagta ctttcaagac ctataagtat acatgatata gatgaagaag ggataacttt
     2881 tctttataaa gtagtaggag aaggaactca aattttgagt aacctaaagg taaatgatac
     2941 tataaaattg gaaggccctt atggtaatgg atatgctaag gttgatggaa aggtagctct
     3001 agtaggagga ggaatcggtg tagctccact atatttggtt gctaaaaata taaaaaattg
     3061 tgatgcatat cttgggttta gagaagatgt tatattagaa gatgaatata aacaagtatg
     3121 taataaagtc tatacaacag taggaaatac ttttgtaaca gatataattg atgttgagaa
     3181 atatgattat atattaacat gtggtccaac tccaatgatg gaaaaattag ttaagatggt
     3241 agaaggtact aaaacgagaa ttatggtatc tcttgaaaac catatggctt gtggagtagg
     3301 tgcctgttta gtatgtacgt gtaaaactaa tggaggaaat aaaaagactt gtaaagatgg
     3361 tccagtgttc tggggagagg acgtgatttt taatgggtaa tttaagtgtg aaatttggaa
     3421 gtgtagaatt taaaaatcca gtaataatgg cgtctggaac ttttggattt ggaaaagaat
     3481 ataatgagat ttatgatata caaaaacttg gtggtataag tagcaagggt cttactttaa
     3541 ataaaaaacc tggtaacaat ggtatgagag ttcatgagac accatccggt atgatgaata
     3601 gtgttgggct tgaaaacccg ggagttcaag gatttataga ctatgaatta ccttttttta
     3661 gtaaactaga cttagtcaga atagctaatg ttggaggtgg gacattagaa gactacttat
     3721 taggggttca aatgttaaat gacaagccta tagacattat tgaattaaat atatcttgcc
     3781 caaatgtcaa agctggtgga atggcttttg gtataaaaaa tgaagtggca agagaagttg
     3841 ttcgtgaggt aagaaacata acgaaactgc ctttagtaat aaaattatca cccaatgcag
     3901 aagatattgt aggaatggca aaagtttgtg aagaagaagg tgctgatgga gtttcattag
     3961 ttaatacttt taaagcaatg gcaattgata taaaaagtag aagaccagta tttgaaaatg
     4021 tatatgctgg gttatctgga ccagctataa aacctatagc tttgagaatg gtacatgaag
     4081 tgtgtaaaaa tgtaaatata cctgtaatgg gaatgggcgg aataactaaa gccacagatg
     4141 ctatagagtt tataatggca ggagctactt gtatccaagt tggaactgca aattttatga
     4201 atcctagaat aggtattgaa ataatagatg gaattaatga atttatggat agagaaggaa
     4261 taaaaagttt agacgaaata agaggaatta tataaggtag aataaatagt attttaaaga
     4321 taaaaaatat agattaaata aacaataatt ttataacatt aacatggagg aaaaataata
     4381 tgagtaaagt agatgtagtg gatatattaa agaaaagtga tgcattgttg gagggacatt
     4441 ttttattatc ttcaggaaaa cacagtaata gatacgtaca atgtgcaaaa gtattaagat
     4501 ttccagaata tgctgctgag gtattaagta cagttgttga acaaataaaa gacttagata
     4561 tagacttagt agtaggacca gctatgggtg gagtaatagt ttcttatgag ttaggaagac
     4621 aattaggaaa agaagctgta tttactgaga gaaaagacaa tacaatggag ttaagaagag
     4681 gatttgaagt taaaaaagga gcaaagataa taattgctga agacgttgta actactggta
     4741 aatcaactat ggagacaaaa agagtattag aagccttagg tggagaagtt gtaggtgttg
     4801 catgtatagc agatagaact aatcatgata taggtatgcc tatatatagt gctataaaac
     4861 ttgatattca agtttatgaa tctgatgagt gtcctttatg taaggaagga aaattaccag
     4921 ttgttaagcc tggaagtagg gagttcaagg aattagggat gtaataaaaa cttaattatt
     4981 tatagtgtta cttaaaaaat gtaaattttt tagagtaaaa atttgtaata taacatataa
     5041 cttaaaaata aaaagcagtt agccttataa atgaaggtta gctgtttttt ttgttaaaaa
     5101 tatatttaca aaataagaga tagtagcagt acaatataag tataaggtaa aagtattata
     5161 tattagggag gggtaagtat gaaaaatcaa ttaaatgaaa atcataaaaa ggttgtattt
     5221 tttggagtag gtgctgtagg agctactttt gcagaacaat tttttaactc taaatatgat
     5281 tttaaaattc tttgtgataa tgaaagaaag aaaagatatt tagaagaagg atttataata
     5341 aatggaaaaa ggtatgattt tgattatgta actaaagatg agtataaaca agaggctgat
     5401 tttataatta taggtctaaa atataataat ttaaaagaaa atataaaaga attagatgga
     5461 ttagttggaa aaaatacagt tataatgtct ctgctaaatg gagttgatag cgaagagata
     5521 ataggagaaa gatttggaat tgaaaaaatg gtatattcat atgttaccaa tatagatgca
     5581 aagaaaatca ataataatat tatacacact actaatggga taattgtatt tggtaacaaa
     5641 gataatagtg aagatagaaa aactaatata ataaccaaag tctttgatga tgtaaatata
     5701 gaatatactt tatcaaaaga tattcaacga gatatgtggt ggaagtacat ggttaatatt
     5761 ggtgtaaatc aaacttcagc tatacttggt gcaccttatg gagtttttca gagttctgag
     5821 catttaagag aattagcaaa atctgcaatg agggaagttg ttgctatagc acaagcaaaa
     5881 gacatatctc ttacagaaga tgatgtggaa cattcattac atagaatact agaacattca
     5941 aaagaaggaa gaacatcaat gcttcaagat gtggaagctc atagacttac agaagtagat
     6001 atgttttcta agaatatctg caaacttgga aaaaaatata acatacctac tcctataaat
     6061 cagactttct tttatatgat aaaagtaatt gaaagtagat tttaaagggt ctttgtaaga
     6121 aatattgata attattgatt ttaaatttta ttaatgttat atactattta agttactaaa
     6181 tatttttaac tcttcaatga aaatagttgc tgcttttgat aaagttatat cttttggaaa
     6241 tatatatgag aaatgtctgt tgtagctgct tccaagctct atgacagaca gctcattgtt
     6301 tagaacaggt aagcttgtca ctagattaga gactattgta attcctaaat tatttctaac
     6361 agattctttg acagcataat tacttccaaa aaccatcata ctttttggaa taatttcttt
     6421 aacacttaaa aacatatcta aataatctct ggttccagaa ccatcctccc tgactatcca
     6481 tttttggttt tgaagtttat caaaactaaa atcttttaat aaatgactct tgtatggaag
     6541 tgctaaaacc atcttatctt caaaaaaata ttcttgaata aatgaagggg aagagcaagt
     6601 gccttcaata agaccaatgt ctaaaatata atccttaaca tgagaagata cgattgatgt
     6661 attttttata aaaacttcta catctatgtc aggatacttt ttagaaaata atgctagaaa
     6721 atttggtaaa atatactctc caatagttaa acttgcacca atctttaagt gaccagttat
     6781 agcatctgaa acttggctta catcatgata agtaatattt aaaagattta atatttcttt
     6841 agctcttttg tacaagattt gtccactttc tgttataaaa atggtttttt gtttaataga
     6901 cctgtttata agcacaactt taaaataatt ttctagattt tttatgtgtt tacttacact
     6961 tggttgtgat agatttaaat attccccagc tttagtaaag tttttgtatt caacaacagc
     7021 tacaaatgtt tttaattctt caaacaattt tttgtctcct ttacctaatg aaattttaca
     7081 ttttttatat actttctaca gataattata ctatatataa taaccattaa aaacattaat
     7141 cattacgata ttatatattt attttactta tggttattac aattatatac tattgctatg
     7201 aagaaaacta tattaattat aaggtaggta tattatgtta aagaaaaaaa gcagtttgac
     7261 attaaaaact ataagggaaa tattaccagg actgtttgta tcagtattag taggttatat
     7321 tagtatattt atttctattt taattcctaa ggtaggggca gcatcaattt ctatattttt
     7381 aggtatgttt gtaggaaacc tatttttaaa tcaaaaggta tttcaaaaag gttataaatt
     7441 ttcagagaca gatttgttat catattcaat agtactatta ggagcaacac tcagtgtatc
     7501 taccttaata gacttaaaag tttcaggaat tttatttata atattgcaaa tgacaataac
     7561 aataatagct gcactttata taggtaaaaa actaggattt gaagaaaact ttagatttct
     7621 aatggcaagt ggaaatgcag tatgtggttc ttctgcaata ggagcaacag ttccagtggt
     7681 taatgcgaca gataaagaaa agggaattgc agtaactatt gtaaatgtta ctggtatatt
     7741 tcttatgttt ttacttccaa taatatctca atttttatat agccatgaac ttgtaaaaac
     7801 ttcagcaatg ataggtggaa ctttgcaatc aattggtcag gttgtagcaa gtggagcaat
     7861 tgttggagaa aatgtaaaag atttagctac tatttttaag atagtaagag taatatttct
     7921 agttgttgtt gtattagtat ttggacatat gaaaaatcaa tctaataggg aaattcttga
     7981 agaagaaaag gatgagataa aaaagaaaaa agtgactata ccatggtatg taattggatt
     8041 ctttataact tgtgcacttt tttcaatgaa cattatacca caagaagtat ctgtgttgtg
     8101 caaagaaata agtaacaaac tagaaataat agctttagca gcaattggat taaaggttaa
     8161 tgtaaaagat ttagtgaaac aagggaaaga agtttcttta tatggtttat ttgtaggtac
     8221 agtacaagtt gtatcagctg tagttttaat aaaaatattt atatagcata aaaatagtat
     8281 ttgtaatatt aatttgatta actaaaataa tatgatacta ataatttata atataaataa
     8341 aaaatgaatt tcgataagtt taagtgggta taaacttata gaaattcatt ttttatttaa
     8401 ggtaaaaatt taaagtaaaa tattatttta cataataata accttataaa ttttgaaatt
     8461 acttatcttt gtgttaaaat aagtaaaatg cacatattag agctaagttt ttaagtaaat
     8521 aaacaaaatg aaaatataaa tattaaggag atttggtata tgaatgttta tgaaaaaggt
     8581 aatggagtta tactagaagg agtaacagat tttgacccag tacatatatt tgagtgtggt
     8641 caatgtttta gatggcacaa acaagaagat ggctcataca ctggagttgc aaaggggaga
     8701 atattaaatg tcaaaaaaga gaatgataaa atatatttaa ataatactaa tttaaaggag
     8761 tttaacagca tttggtataa ttactttgat ttaggaactg attatgcaga aataaagaat
     8821 aagctaaaaa atatggatga atatttaaat aaggctactg aatttggatg gggaataaga
     8881 atattaagac aagatggatg ggaaatgctt atatcattta taatttcctc taataatagg
     8941 attcctatga tacaaagagc gatagaaaat ttatcaagaa agtttggtaa gtatataggt
     9001 gaatatgaag gcaatgaata ctatgcattc ccaacaccag aagaactaaa taaggcctca
     9061 caagaggaaa taagagcatg tcaaacaggt tttagagata agtacataaa aagtactaca
     9121 caggcagtta tagagaataa tgatgaggta tctgaatata caaatttaag tacagaggat
     9181 tgtagaaaag agcttttaaa atttaatgga gtaggaccaa aagtatgtga ttgtattgca
     9241 ttatttggta tgcaaaaata tgattctttt ccagttgatg tttgggttaa gagagttatg
     9301 caagaatttt atatagatga agacatgagt ttacctaaaa tgagaactta tggaatcgat
     9361 aaatttaaag aaatgtctgg atttgctcaa cagtacttat tttactatgc aagagaactc
     9421 ggtataggaa aataaattaa gggggagaaa ttatgggagt aataggaaca atatttctgt
     9481 tctatttaat tatatcatat ctagctggag ccataatttc tgtgattata ttacttgaaa
     9541 atagagaccc tgcaaaaaca atgtcttggc tattgatgtt tattatattt ccaggtgtag
     9601 gtttaatgat atatgctatt tcaggtagaa atattaggaa aagaaaatta tttaagactc
     9661 aaaaactagc taataatata aaagaaaaga aactatttga tacattagaa aagataacag
     9721 aaatagttga attagaaaaa gaatctataa aacaaaataa gcttttaaga gatgaagagg
     9781 atggtagtta tagaaaaagg gtaataaata tgttgttaaa gactggtatg tttcccttta
     9841 ctaaaaataa taaagtggat gtatttgtag atggaaatga aaagtttaaa agacttatag
     9901 aagatataag agaagctaaa gaccatattc atttagagta ctttatcatc aaagatagtg
     9961 aaataggtag ggtgttaaaa gaagaactta taaaaaaggc taaagaaggc attaagataa
    10021 gaattttata tgatgatgta ggatgctgga gattttggtt taatagaaaa tttttccgag
    10081 aaatgaggga agtaggtata gaaattgcag catttttacc tacaaaattt cctataatag
    10141 gagggaaatt aaactataga aatcacagaa aaattgttgt tattgatgga ataattggat
    10201 atacaggtgg aataaatata ggagatgaat atttaggcaa aaatgataag tttggatatt
    10261 ggagagatac tcatattagg attaagggta tttctgtata catgcttcaa atgacatttc
    10321 tgattgattg gtattatacg acaaaagaag tattagtgac taaaaattat ttcccaagtg
    10381 tagggaatgt tggtgaaagt atgatacaag ttgttgcgag tggtccagac agtgactggg
    10441 aagatattca ttatgcttat ttttctgcaa tatgtcaggc tagaaaaaat gtttatatag
    10501 aaactccata ttttattcca gatgaaagtc tactaaaagc tataaagagt gctgctctta
    10561 gtggagttga tgtgagaata atatttccta aaattgcaga ccacaagata gtaaatatag
    10621 catcttattc atattttgaa gaaatattaa gagcaggtgg taaagtttat ttatataata
    10681 aaggatttat acattctaaa gtcgtaataa tagatgataa aattgcttct gcaggtacag
    10741 ctaatatgga tttaagaagt tttatgctta actttgaggt aaatgcattt atctatgatg
    10801 aagaagttat tagggtaatg acagatgatt tctttgagga tttaagtcac tgtgaagaac
    10861 ttaatttgga ggtatttaag aatagaaata tcatacaaaa aatcaaagaa tctgtagcaa
    10921 gattattttc accaatatta taaaaaatta ttaaaaaagg tattgaaaat tatgtgtgat
    10981 tggtatatta taataattgt tagcactcta ttagatagag tgataataaa taaaaagtat
    11041 atatataaat ctacatatat aagtagtatt aatttaaaaa taactaaaaa cataggcgaa
    11101 ctagtctata cttaatattt taggaggcgt gaataatgaa aataagacca ttagctgaca
    11161 gagtagtaat taaaaaagta gaagcagaag aaaaaactgc aagtggaata gttttaccag
    11221 gagcagctaa agagcaacct caaatagctg aagttgtaga agttggacca ggtggaatag
    11281 ttgaagggaa agaaataaaa atggaattga cagtgggaga taaggttata ttccaaaaat
    11341 attctggaac agaagttaag atagaaggac aagaatacac aatactaaga cagagtgatg
    11401 tattagctgt tattgaataa atatagaata aatttattag gaggggttta aaatggctaa
    11461 agaaattaaa ttttcagagg aaacaagaag agctttagaa gctggtgtaa ataaattagc
    11521 agatacagta aaagtaacat taggacctaa aggaagaaat gttatattag ataaaaaatt
    11581 tggttctcca cttataacta atgatggagt aactatagca aaagagatag aactagaaga
    11641 tagatttgaa aatatgggtg ctcaattagt taaagaagta gcaactaaaa ctaatgatgt
    11701 agctggagat ggtactacaa ctgctacagt tttagcacaa gctataataa gagaaggttt
    11761 aaagaatgta acagcagggg ctaacccaat acttttaaga aaaggaatac aaaaagcagt
    11821 aacagtagca gtagaagaat taaaaaatca atcaagaata gtagaaacac aagaggctat
    11881 atctcaagtt gcttctatat ctgctggtga tgaagaagtt ggaaaattaa tagcagaagc
    11941 tatggaaata gtaggtaaag atggagttat aactgttgaa gagtctcaaa ctatgaatac
    12001 tgaattagat gctgttgaag gtatgcagtt tgatagagga tttgtttctg catatatggt
    12061 tacagatgta gataaaatgg aagcagtttt aaacgaccca tatatattaa ttactgataa
    12121 aaaaatatct aacatacaag aattattacc agttcttgaa caaatagttc aacaaggtaa
    12181 aaagttattg ataatagctg aagatgtaga aggtgaagca ttatctacat tagtagtaaa
    12241 taaattaaga ggaacatttg atgtagtagc agttaaagct ccaggctttg gagatagaag
    12301 aaaagaaatg cttcaagata tagcaatact tacaggtgct caagtaatat cagaagaatt
    12361 aggttatgat ttaaaagaag ctgatttatc tatgctaggt agagcttcat ctgttaaggt
    12421 aactaaagaa agtactacaa tagtagatgg ttctggagat aaaaaagcta tagaagatag
    12481 agtaactcaa attaaacatc aagtagagca aactacttca gattttgata gagagaaatt
    12541 aatggaaaga ttagctaaac ttgctggagg agtagctgtt gtaaaagttg gagctgctac
    12601 agaagtagag ttaaaagaaa gaaagttaag aatagaagat gctcttaatg caactagagc
    12661 agctgtagaa gaaggaatag tagctggtgg aggaactgct tttgttagtg ttataccagc
    12721 aataggaaca ctaattgaga gtttagaagg agaagttaaa ctaggtgctc aaatagttaa
    12781 aaaagcgtta gaagagccat taagacaaat agctataaat gctggtcttg aaggtgctgt
    12841 aattgtgcaa aatgttgtta attctgaagc agaaactgga tttgacgcat tgaatgaaaa
    12901 atatgtaaat atgatagaag ctggtatagt tgacccaact aaagttagta gaagtgcttt
    12961 acaaaatgct gcatcaatag caagtacttt cttaactact gaagctgctg ttgctgacct
    13021 tcctgaaaaa gaagatgcag gaatgccagg aatgggtggc ggaatgcctg gtatgatgta
    13081 atttaaaaaa tttaaaatat aaattttaat ataaagaact atttcgactt agattttaaa
    13141 ctctaagttg aaatagttct tttgtatata tctaattttt gtatatctat aaattcaata
    13201 cacatattta gataatcagt atctagtttg taataatatt ttagagtagt attgttattt
    13261 tttaggttat gattttagat atatattaat taaggtataa ttgttgatag agaataaatc
    13321 aaatttaaaa ggatgcgata atatggactt aagaaaattg cttgaagaag ttaaaaatga
    13381 aaatattgat atagattttg cgttagaaaa gttaaaagat ttaccttatg aagaccttgg
    13441 atatgcaaat atagaccatc atagagaatt aagaaatggg tatccggaag ttatttattg
    13501 tgaaggtaag agtgatgagc atattttagg gattattgat aaaatgaatc aaaaaagttc
    13561 taacatattg gggacaaggt gcagaaaaga aacttttctg aaaattaaag aaatctataa
    13621 tcatgctgag tacgaagagg cgtctaaaat tcttaagatt caaaatcatg atatagagaa
    13681 tataggaaaa ggtaaaattg tgattgctac agggggaact tctgatatac cagtagcaga
    13741 cgaagcttat catacagcta agtttttagg taatgatgtt gatagaatat atgatgttgg
    13801 agtggcaggt atacataggc ttttaaataa aagacataaa atagatagtg ctagagtaat
    13861 agtagccgtt gcaggtatgg aaggtgctct tgcaagtgta attggtggac tggttgatgt
    13921 tccagtaata gctgttccaa cttctgttgg atatggagca aattttggag gattagcagc
    13981 attactagct atgttaaata gctgtgcatc tggaatatct gttgtaaata ttgataatgg
    14041 atttggagca ggatatttag catcaatgat aaataaactg taaatacatt gatataaatt
    14101 aaacaaaact atataataaa atttaatttc tattatattt ttactattaa aattgaggag
    14161 acatatatga gtgaaaaaat actttatttt gatataataa gcggtatatc aggggatatg
    14221 acccttgcaa gtttattaaa tttaggtgtg cctaaggaaa tttttttgga ggaaattcat
    14281 aaactaagaa tgagcgatga atttaatata aacatcagct cgaaaactga aaatggaata
    14341 gtaggtacaa aagtagaagt aattacaaaa gaaaaacata cacatagaaa tttagtggat
    14401 atatttgagg ttatagataa aagcactcta aacgaaaatg taaaatctaa agcaaaaaaa
    14461 atatttatgg taattggaga agcagaagca aaagttcatg gaactacaat agacaagata
    14521 cattttcatg aggtaggggc tatagattca attgtagata tagtaggagc ttctatatta
    14581 gttgacttac tttgtgtaga taaagtttgt gctagtacag tgtctgttgg ttcaggattt
    14641 gtaaaatgtg aacatggaat tatacctata cctgcacctg caactataga aattttaaaa
    14701 ggtgtgccaa ttaaattaaa taatgtaaat ggagaatgta caactccaac aggagctgca
    14761 atcataaaag tcttgtgtga tgagtttgta gatgagcttg aattttctcc aaagcaaatt
    14821 ggatatggta taggtcataa gaagtttgaa gtaccaaata tactcagaac tgtattaggt
    14881 gaaaaaaaaa agaagaatta atttatgaaa ttagtgcaaa cattgatgat atgtcttcag
    14941 aaatctattc ttatttgtat gaaaaaatat tagatgagaa agcattagat atttatacag
    15001 agagcattta tatgaagaag aatagaccag caacaaaagt atcaatttta tgtaatgaat
    15061 cagatttaga aaaatttatt aaaatattac ttttagaaac tagtactttt ggagtgagat
    15121 ataatgcata taatagagaa attttagata gaaaatttgt aaaattagat acaaaatatg
    15181 gagtggttac agtaaaacta ggatattata aaggaaaact agtcaaggct actccagaat
    15241 ataatgaatg taaaactata gcaaaaaatg aagatattcc tataaaaaaa gtgtttgctg
    15301 atataaattt tagaataaaa gaagaattta gtgaaaactt attgacataa gtaataaatt
    15361 ttgataaatt aatattcagc cttattaaat aaaagtaaac agaaaaaaat atataaaata
    15421 taaactcact cgtatatgct cagaaatatg gtctgagagt ctctaccaag ataccgtaaa
    15481 tatcttgact atgagtgaaa ttattatacc aaaagaccta gttaagtaaa tgtgtaggct
    15541 gagttaaact ccctatgtgt tggctataag ataaaggtat tttggaagcc tgtatagtaa
    15601 tttcactttt gctagaaatt atggaagtta ctatacaggt tttttgtgta aaaagattta
    15661 atataattta gactgtattg tgttaaaata aatatataaa aactatatta tacaaggaga
    15721 gatgtgaatt tatgaaacat gaattagtac ttgtaataga ttttggagga caatataatc
    15781 agttgatagc tagaagagtt agagaaaaca acgtatattg tgaaatactt ccatgtactg
    15841 caagcataga aagaataaaa gaaaaaaatc ctaaagggat aatatttaca ggcggtccaa
    15901 atagtgcgta tttagaagat tctccaacaa tatcaaaaga aatatttgaa ttaggagtac
    15961 caatacttgg aatatgctat ggtattcaaa taatgtctca tgtattgggt ggagttgtaa
    16021 gaaaaggtaa taaacaagaa aaagaatatg gaaaaacagc tataacttat ggaaaatcat
    16081 cattatttga aggaataact acaaatagtg tatggatgag tcatacagat ttaatagaaa
    16141 aagttccaga aggatttact atagttgcaa atactaatga ttgtccagta gcagctatgg
    16201 aaaatgtaga acgaaaccta tatggagttc aattccatcc agaggtagaa cactgtttgg
    16261 aaggggacaa gatacttaca aacttcttat ataatatttg taaagtaaaa ggtgactgga
    16321 ctacagattc attcatagaa gataaaataa aagaattaaa agaaaaaata ggcgataaaa
    16381 aagctttatg tgctttaagt ggaggagttg attcttctgt tgctgcagtt cttattcata
    16441 aagcaattgg ggacaactta acttgtatat ttgttgacca tggtttactt agaaaaaatg
    16501 aaggaaatga tgtagaaaga atatttagag agaaatttga tataaattta ataagagtta
    16561 atgcagaaga tagattctta tctaaattaa aaggagtttc tgaaccagaa gctaagagaa
    16621 aaataatagg tgaagaattt ataagagtat ttgaagaaga atcaaataaa ttaggaaaaa
    16681 tggatttctt agtacaagga acaatctatc cagatgttat agaaagtgga catggaaatg
    16741 ctgctactat aaagtctcat cataatgttg gtggaatccc agaagatgta gacttccaag
    16801 aaattgtaga accattaaga gagttattta aggatgaagt tagaaaaata ggattagagc
    16861 ttggtataga agaaggatta atatttagac atcctttccc aggaccaggt ctaggaataa
    16921 gagttatagg tgatgtaact aaagaaaaat gtgatatatt aagagaagct gatgctgttt
    16981 atatggacga gcttagaaaa gcaggtttat acagagaaat atggcaagct tttgcaacat
    17041 taccagatgt taagactgtt ggagttatgg gtgatgaaag aacatatgct tatttagttg
    17101 gtttaagagc agttacttct tctgatggaa tgacttctga ctggtataag atgccttatg
    17161 atgtattaga aagaatttct aatagaataa taaatgaagt tgatggagta aatagagtag
    17221 tttatgatat aacttctaag cctcctggaa ctattgaatg ggaatagtaa ataaaaaagt
    17281 cgtaagcctt gtattttcaa ggtttgcgac ttttaaaaaa atctttgtga tactaaaatg
    17341 atactatata tttaaattat ctagtttttg ggcaacttca agttgcttat ttgggtataa
    17401 atgtgaatat gtattccaag ttgtttctac tttttcatgc cccaatcttt ctgctatagt
    17461 taatatattt acatccatat ttaccaataa tgacgcatga gaatgtctca aatcatgcac
    17521 tctaattctt tttacattag ataacttaca gcatctatct agctctttag aaagataact
    17581 tttagcaaat ttaaatattc tttcattatc ttttaagtca tatagcttag atagataatc
    17641 ttttatatta ttatataaga aatgtgggat agggacaaca cgtttacttt taggagtttt
    17701 aggagaagaa acaatatcct ctccatttag tcttatgtag cttttttcga cgcttatttt
    17761 attttcgaaa atatcttttg gagtaagagc taacaactcc cctagtctaa gacctgtcca
    17821 aaataaaatt tcaaatgcta gtctaggttc tgatttcttt tcaaattcaa taaatttttt
    17881 aaactcttct aatgtccaaa agttcatttc atctgcgttt tttttaccga tagaacctgc
    17941 caaatgagca ggattagaag gtaagttata atatttaact gcataattta atacagcaac
    18001 aagctggttg tttatcgttt taatatatgt ttggctataa tcacttttta ataattcgtt
    18061 ttgccatctt cttatatgtg ttgcagtaat ttcatttatt tttagacttt taaaaaaagg
    18121 taaaattcta aagtttatta aatacttttt agtttctaat gtagacaatt ttaatctaga
    18181 agacatatcg tgcatgtatt cttctatgag actttcaaaa ctcatatcag tactcatttt
    18241 agatttattc aaaaattctc tttcaaactc tagagcttct tttttagttt taaaacctct
    18301 cttaattttc tttttcctat ccccatcgaa gtctgtaaaa taaaagctag cataccaact
    18361 ttttctttgt tcgtctttgt aagcaggcat tttagacacc cccaattatg tcattttaat
    18421 attacaataa ttataacatg ttagatatgc tatctaaata attgttttat tttcctcttc
    18481 tttttatttc ttgaagcttg taatatttac acagttctaa gaagtcttct tctagtgcta
    18541 tagataagtc taatatcgta gatataccta tatctttgta ctcttgattt tctagttttg
    18601 atatataact tctgttacgt ctcatttttt ctgctaattc tagttgtgtc attcttttct
    18661 tttttcttaa ttttttcaac atttattaaa atccaccttt taattatttt tatgacattt
    18721 ttataattaa tagtttgtgt aatttcttgt aaaaaatgtt cctcattaga acattttttg
    18781 gtggaaaaat gtgttaaaat gtagttaaga aattaaaag
//
LOCUS       DAGSWI010000077        18618 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.077, whole genome shotgun sequence.
ACCESSION   DAGSWI010000077 DAGSWI010000000
VERSION     DAGSWI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.077"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(141..518)
                     /locus_tag="MJF80_002413"
     CDS             complement(141..518)
                     /locus_tag="MJF80_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0281070.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
     gene            complement(705..1676)
                     /locus_tag="MJF80_002414"
     CDS             complement(705..1676)
                     /locus_tag="MJF80_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0281071.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            complement(1710..1907)
                     /locus_tag="MJF80_002415"
     CDS             complement(1710..1907)
                     /locus_tag="MJF80_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBZ0281072.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            complement(1975..3186)
                     /locus_tag="MJF80_002416"
     CDS             complement(1975..3186)
                     /locus_tag="MJF80_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HBZ0281073.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            3467..4069
                     /locus_tag="MJF80_002417"
     CDS             3467..4069
                     /locus_tag="MJF80_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBZ0281074.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            complement(4325..5296)
                     /locus_tag="MJF80_002418"
     CDS             complement(4325..5296)
                     /locus_tag="MJF80_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBZ0281075.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(5322..6803)
                     /locus_tag="MJF80_002419"
     CDS             complement(5322..6803)
                     /locus_tag="MJF80_002419"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBZ0281076.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLSYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENNENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            complement(6822..6970)
                     /locus_tag="MJF80_002420"
                     /pseudo
     CDS             complement(6822..6970)
                     /locus_tag="MJF80_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            complement(7139..8125)
                     /locus_tag="MJF80_002421"
     CDS             complement(7139..8125)
                     /locus_tag="MJF80_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBZ0281077.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            complement(8510..10264)
                     /locus_tag="MJF80_002422"
     CDS             complement(8510..10264)
                     /locus_tag="MJF80_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HBZ0281078.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVVKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKNYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            complement(10482..11372)
                     /locus_tag="MJF80_002423"
     CDS             complement(10482..11372)
                     /locus_tag="MJF80_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBZ0281079.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEVISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            11521..12381
                     /locus_tag="MJF80_002424"
     CDS             11521..12381
                     /locus_tag="MJF80_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBZ0281080.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            12837..13403
                     /locus_tag="MJF80_002425"
     CDS             12837..13403
                     /locus_tag="MJF80_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBZ0281081.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            13443..14024
                     /locus_tag="MJF80_002426"
     CDS             13443..14024
                     /locus_tag="MJF80_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBZ0281082.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(14081..14998)
                     /locus_tag="MJF80_002427"
     CDS             complement(14081..14998)
                     /locus_tag="MJF80_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBZ0281083.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            15128..16759
                     /locus_tag="MJF80_002428"
     CDS             15128..16759
                     /locus_tag="MJF80_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBZ0281084.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            16762..17709
                     /locus_tag="MJF80_002429"
     CDS             16762..17709
                     /locus_tag="MJF80_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBZ0281085.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(17994..18257)
                     /locus_tag="MJF80_002430"
     CDS             complement(17994..18257)
                     /locus_tag="MJF80_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBZ0281086.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(18356..18601)
                     /locus_tag="MJF80_002431"
     CDS             complement(18356..18601)
                     /locus_tag="MJF80_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281087.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
BASE COUNT         6328 a         2817 c         2304 g         7169 t
ORIGIN      
        1 ttccaattat tttaggcatt ttactaatta gagaacctat ttaatattac tttatgaata
       61 gtgagtagct gattaaataa taccatttat ggttttcagc catagattaa ttaaattctt
      121 agagttctat cctgatataa ttactcttct tctaaatttt taaaatgact ttcaaataaa
      181 tccaacatat ctttatcaac aacttcatca tcatgtagtg ctgaaattaa actctgtaaa
      241 gaattgtcgt gtatattgct taaaaaatct tttgtctcaa cacttaaata ctccttatgt
      301 ccaacaacta ttgtataatg agtgtatcta tctatttttt ctgcacttaa aaaatgtttt
      361 ttaacaagtc ttgataaaag tgtcaatgta gttgtttgtt tccaaccata ttttcgttcc
      421 atagcttcaa tagtttcttt tgatgttact gtatcatttt tttcccaaat aaacttcata
      481 acttttaatt ctgcttgtgg tatttttgaa attgtcataa ccttacgccc ctctcaaact
      541 tttattaaat taatactatc gcaatatagt ttttaagtca aagattttgt cgataataat
      601 attttccaat ttttaccata gggttatttt ttatataatt ctatgataaa ttacataaaa
      661 aatatttatt ttacatcata aaattagaaa tgtaaatagt atgattattt agtcttatct
      721 ttaataaaca atccagtaaa tttatacatc caaagtgcat tcataaacaa caataaactt
      781 gttgaaaaaa atactgttct aatacctaaa tgtgctgata tttgtccccc taaaaatgct
      841 cctgatgaac agcctatata ctgggcagat gcattatatc caaatatttt tcccacatat
      901 tcttctggtg caattttttt aagcaagctg ttaacagaag gattaagccc tgcaactgac
      961 aaccctaata aaaatctcaa caacatcagt tgccatgcag tttttacaaa agcttgtggt
     1021 ataaatataa tcccagccca taaaagtgaa attaacaata ctttttgagg acctatttta
     1081 tctgacattt ttccaagttt ttgagctgct aataaatttg ctaatcctga tgcagaaaat
     1141 gtcaatccag ctattaaagc tacatttgat gcaaaatttg taagttgtga tatatatatg
     1201 gttacaattg gttctataga gtataaagct agttgagtaa tgaatgtggt tacaaaaata
     1261 gatgcaatta aaaatttatt tggcaatata tttaattgtt ctttaggtat agaaattgat
     1321 tcttttattt ctttaggttt aaaattatct actacaaata aaatagagat gataaatgat
     1381 acaaacaaca atcctcctgt tataataaat gtacttttaa gacctaagat tgattctata
     1441 tatcctccta ctgttggacc aattaatgag ccagtagttg atgcagttgc aagagtaccc
     1501 aaagcccatc ctgaatgatt tttatctgtc tgtgttgcaa taagagtagt acatgcagta
     1561 gcataacccg taaaaactcc ttgaagtatt cttaatccta ataactgata gacattttga
     1621 acaaacgcca tggtaaatat aactattccc atcccaatac ttgcacgtaa aagcattgat
     1681 tttcttccat atttatcagc agctttccct caaattggcg aaaagaatgc tgctactaca
     1741 aaggtacatc caaaaattat acctgaatac tgttgtatta aactaacatc agttaccccc
     1801 aactgtttaa tatataatgg aagtataggt gctatttggc tcatccctat agaactaaaa
     1861 aacataccta tccaacatac aaataaattt cttttccaca attccatata gaattacacc
     1921 ccctgcattt taaatttttt caataatact aagtttatta atttatgcta ttgtttactc
     1981 aatatatcca tcttttcttt tataatattg tttaggaaat tcataacttg aaaataatag
     2041 cggtgataaa gaataggagt aacttccagc attactaatt gaaatcaaat ctcctatgtt
     2101 tgcttttttc aatttaacat tatttgcaat tacatctaat gatgtacaga gattcccaac
     2161 aacatttaca tactcttctt cttttaaatt atttaatact gaaagagaaa aggcatgatt
     2221 tgatgtatat aatggctctt gaccttctaa ttctactcca caagtaatat tttcaattaa
     2281 attagaaata ataggtctca taaatccatt aagtccattt tttacaataa gatatttttt
     2341 ccctcttgat actttaatat ctacaatttt aacataataa ttacctgatt tacaaactat
     2401 gaaacgtcct gattcaataa gaagtcttga ttttagtgtg tttgaattag taaaagcaat
     2461 cttttgagtt aattttgaaa gctctttaag attaagtgga ttttctctaa gattatcata
     2521 agctactcca agtccactac caaaatttat aaattcaata ttaatatact tacttttatt
     2581 taaatttaaa gctataaaaa aacatttttc ataataatgt cctaactttt caaaatccaa
     2641 tacctgagat ttaatatgta tatgaatacc aactacagat acataatcac acatacttaa
     2701 catcttaaaa acactgctca tatcttctat atctatacca aatttgcttg atttagcact
     2761 atcatcatcc atagtaaaat caggatttat tcttattcct attttaactt tctgattatg
     2821 ctttttagat atcttattta taagttctat ttctgtaata ctatctgcaa taattatgca
     2881 tttatctaaa acagtttcta tatcaatctc tgttttgcct ggtgatgaat aataaatatc
     2941 atctttagaa actccacatt ctgatgaaat cataacttca ttagttgaag aagcatctgc
     3001 tccaaaacct tgctctgcaa tgctttttac aattttgtta tatggatttg tttttataga
     3061 atataaaaaa tcaaaaccta aaatattatt tttcaattcc ttacattttt caattatatt
     3121 agtctcatca tatatataac atggtgcatt ttcttctata aatttatagt cttcatttga
     3181 gtacatattt tttacctcca agattttaaa tataatcata aattaagcaa ttataatcct
     3241 ataagataat tttacaataa attaccatag ttaactacac acatatatgt aaaaacatac
     3301 ctaaaatatt tttaattata gatacattcc aacttttatt gataaaacaa tttgtttcaa
     3361 ttttattaca aatatacaaa gtgtattgtt gttaacgtat tctcatagta tcctaaaact
     3421 atattaagca ctaaaaacta ttttaaaata ctgtggaggt atcaatatgg aagtatgcat
     3481 tcttatggga agtcctaaga aaaaaggtaa tactgctgct attttaaaac cttttattga
     3541 ggaattagaa aattataatt caaatattga agtaatatgg ttatatgatt gtaaaataga
     3601 gccatgtatt gcttgtaaaa aatgtcagga taacttattt gattttggat gttattgtaa
     3661 agatgatgtt cagaaaattt tcaagaaaat tcttatgtgt gatttaattg tttttgctac
     3721 tccaatttat tcttggtact gcacttctcc aatgaaagca ctacttgaca gattggtgta
     3781 tggaatgaat aaatactatg gtaatgagaa aggccccgct ctatgggcag gaaaaccagt
     3841 agcacttatt acaacatgtg gatatagacc tgaaaagggt gctgatattt gggaaaatgg
     3901 tatgaagcgc tactgtaagc attcacagtt aaaatatata ggaatgcttg ttgaacgtga
     3961 tttaggtaaa cctaatttta tgaataaaga aaaagaagaa cattctcgtc aatttgcaaa
     4021 acaaatttat tatattttag ataattcaat tgaagtagat aactgctaaa gacataatac
     4081 tccaagtaaa gtattgataa tataaataaa tcttacataa tttgcttatt taaaataaga
     4141 tatatattct tctaaaaagt aacaataatt aaaatatttt ttatcatttc aacaatgata
     4201 aagaggctta tctctatttt gagacagcct ctttattgct ttttatatgt taattatttt
     4261 catagtaaac atccttaaaa actctttaca tgttacttcc ttcaaaacct attactcatt
     4321 cctactataa attttttata aattcagtta tttcttcctt tgttgcatat gaacttatcg
     4381 cacctttaat agttgtacta cgagctgcat aataattaga aaattctaaa tactcattta
     4441 acgtttcttg tttcatttct tttatatcat tcaatgttat ttgtttatac aataaggtgt
     4501 ataaaaatga acctatataa gaatctcctg ctccggttgt atctattgca tttactttaa
     4561 cccctttaca catacctttt acttttttag tgtatgcatc tactccatct tttccttttg
     4621 tatataatac tagttcaaca ttccccacaa agagtttatg taatgatttt tctatatcat
     4681 tttctcctgt tataaattct aattcttcat cagatatctt aacaatatga gcaaaaggta
     4741 aaaattctga tattgccttt ttacaagatt gctcactatc ccataaaggt aatcttatat
     4801 tagggtcaaa gcttattata ctattacttt caagtgcgta ttctattgct tttttatgtg
     4861 ctaatttcat tggactatct attaaatcta ctgaacaaaa atgtaggata tgacaattat
     4921 taaaccactc ttttttaact tcatcagctt ctaaaagcat atctgcactt ggatttctat
     4981 aaaatgagaa atcacgatta ccatcttctt ttaatgatac aaaagcaagt cctgtatttg
     5041 ctttacttgt tctcaataca taatcagtat ttacatttac ttcatttaat acatctatta
     5101 tataatcacc aaatgcatct tttcctaact tagatataaa actcgatttt cctccaagct
     5161 ttgctacaac agcactaaca tttgctggag ctcctcctgc tactcttcta aattctgata
     5221 cattttttaa tttaccacca ttttgattcg gtataaagtc tattaaagct tctcctatgc
     5281 taattacttt tttcatcttt tctacccctt ataatgtgtt tttataattc aataccttgc
     5341 tttaatttaa atttagtata ctcatactct actattttat cactataaac ccttatttga
     5401 ttagaatttt ctttagcaaa tattctacta gtaaatacta cttctccttc atttataaaa
     5461 atttctaaaa tacttctatc cgctagtact ttgatattag tattttcatc aatatttata
     5521 tattcttttc tatttgttcc atattcaaca ccgaaaactt ttttaaagtt gcttctgtct
     5581 aaagttacaa tgtttcctaa tttgttaaat tctaatttta caccttcatc ttgtgatgaa
     5641 aataattcca gcccaaattt attgctgtca atatttttta tattaatatt aagctcatat
     5701 acattttcat tattttctat aatattgata ttattcttta tagtattttg cccacttgtt
     5761 tcatcatatc ttaatttaat taattcatct acaggcattt gcaatagctt attatttttt
     5821 atatttaatt ttcttggaac agttagacaa tgtgcccatt tgtttttatc tgtagggtat
     5881 aaaacttctc ccattccagc ccatgcaaat agtattattt gtgagtcctt tataaatgtt
     5941 tgtggagcat agaaatcaaa tcctttttcc agttctttca ttgtatctat ttcaaatgtt
     6001 aaattatcta aatccatatt accaatagca tatactacat tatatatatt ttgataatca
     6061 aatcctttag gttctactcc ttgtggtgaa aatattaata tatctttttc atctatatta
     6121 atataatctg gacattccca catgtaacca aaatcttcat ctatgttttt tacatttaat
     6181 tctcctttaa aattccaatc tatagagttt ttagattcat atactattat aactcctttt
     6241 ttatcacttg tttgtgctcc aagtaacata aagtatttat cctttctttt aaatactttt
     6301 gggtctctta catgtcctgt atatcctttt ggtatttcac ttactattgg attattttca
     6361 tatttagtta ttttaccatc cttgttcatt atagctaagt tttgatatgc atatctatct
     6421 tctgcactat attttatatt ccctgtataa tacatatgca attttccatc tacctctatc
     6481 gcattacctg aatatgctcc atgagattca taactttcga ttggtattaa tgctacatct
     6541 tctctattcc agtttacaaa atcatcagaa cttacataag cccaatgctt cattccatga
     6601 gttgcatcat atggatacca ttgataaaat acatggtact taccattata atatgataat
     6661 ccatttgggt cattaagtag tccatgttgt ggatgtatat gaaatagtgg tttatattta
     6721 tcatttaaag atatatttct taatttattt aattcttctt ttgtcgattc taatatggtt
     6781 ctatatttag gctctttgta catattttta ttccttttct tttataattt tatatttgct
     6841 ttattattgc tatctaaaac aattcttagt cttattacac aatgtgctac atcttttata
     6901 ttttgtttat tcctatgtac tctaataatt actttgatac tttgttacaa tcagttattt
     6961 ttgcactcat tttgctattc tatcggttta attaaaccgg tttcgaaacc ggtttaatta
     7021 aagattagca taaatttatt tcagatgcaa tatttctttc gaaaaagttt tcctaatttt
     7081 tagaattata gtttttttag taaaaaaata cacattacta atatatgtaa tgtgtatctt
     7141 aatttttatt tattgtactt tcaccttcta ttaattcaaa tttaatctcc tttaatagtg
     7201 gttcttttct ttcttgtatt aaatttacta tcgtatttgc tgctacttta cctgcatttt
     7261 tattttcaaa ctttattgtt gtaagtttag ggcttataat agacaataca tcatatcccc
     7321 caaatccagc tacagatata tcttgtggta tacttttacc tctcttattg atttctttca
     7381 ttgttgctat tgctatatta tcagttgcac atattattat atctggattt tcactttcta
     7441 gcatttctcc acttctttgt atagacgttt cttggtcaaa gtcagtataa aatacttttg
     7501 catcataccc tttatctttt aacccattaa gtactccatt ttttctattt aaaccaactg
     7561 atatatcact ttcatcaaca cctaaatata caatgttttt gtaaccttta ttagcaatat
     7621 attgacccat tttatatcct gctccataat catcattcac tatgctattg ataccatcaa
     7681 ctttttgtcc aactacaact atcggtatat ctaacttttc aatttcattc ttatgttctt
     7741 tagttatttc ggtaccaatt agtattattc catcaacttt tagactagct aactttgata
     7801 tactatctat ttctgacctt atttttctat tagtattaat aattagtgat gtatatttta
     7861 attctttgag ttcttcatct attgccatca taattttaga ttttacaaaa gaatctaaac
     7921 acggtgttat aataccaata aagtatgttt ttttagctct caaactttga gcaaatgcat
     7981 tcggctcata tccatattca tctattattg tctttatttt ctctttagta gaatcactga
     8041 tatctttatt atttagatat cttgatacag tacttttaga tactcctgcc aagttagcaa
     8101 tatcatttat tgttattttt ttcataggtt atcaccttct acatttgata tattttatta
     8161 cattatatta tatatttaaa ataaaatata gttatttgtc cagtcaatac tttttatata
     8221 ctacttgtac tatccatatc cacatccaaa gaattgagcc tttaacatta aattaattgt
     8281 aattttcatt ttttgttgtt atatttaatt tcaattttat ctactatgaa ttgataaata
     8341 ctttaattta tttataatat aaaaataagg ctatgatttt tacaaccata gccttatcga
     8401 taaatactta attatcactt aaaaaataat tttttattta aagaatatat ataaatattt
     8461 taaaattcta taatttttaa aactatataa actttatttt catatgtttt tattctccaa
     8521 ttgatatatt atttatgcca ttatttttaa cttcaataat tggttgtcca tttcctaaaa
     8581 tataattttt agaccaacct tctggcaatt tatttatccc atttccaatt aacttcaagt
     8641 gtaaatttga tggttctttt ttcagattta atttaaatat tccatcaaag gtacctttat
     8701 tatcgcttcc aaataaactt atattgccat ttatattcgt agctgttaca tttacaattc
     8761 cattgtttgc tctaaatttt atttcagatg atatagtttc agctgttaca cctgaaatcc
     8821 cactattagt atttatattg aaaggatttg ttacattgtt atcaacaatg ttagcaatcc
     8881 cgtcattgac atcaacagta gctcctcctt taatattata aagttctact gaaccacttt
     8941 caacatctac tttaaaatta gaatacattt tatcgggaac tccaattttt atcacattta
     9001 ccatcctatc tttatttgca tttatcatat tgtcgccatt catatcttgc aaatcactat
     9061 ctttcttatc tatttttttc ttagcactta caactagctt tccatttttt atattattag
     9121 aagaattttc tttatataca gaaaaagtac aatttttttc attagttaca cttacgtaat
     9181 caaactcaaa tttattggta ttcgtctttt caacaaccac acctaaatta tctgattcta
     9241 taagtatttc agaaacttca tttccttcaa ctgaagttaa gtgttttaaa atatttggag
     9301 tcatatccat attattatat ttaatcatat ctaaacactg tttatatata ttggcatcaa
     9361 tacctccaac ttttgttata cttttagctc cttttaaaat acctttatta cttccatcag
     9421 caactaaaac gattggtttt tctttggcta aaggagataa tgttagtgca tctactaatt
     9481 gatatccatt tgttatataa aaatctgtcg ctccattata aaacttttcc attacctttt
     9541 tattggtctc aaatctatca tttcctccga ttctagttgc tttagtttca tttactaaat
     9601 cctcatttat cgcagatttg ccacctatag cgtaaacatt taaactttca gtcttaaatg
     9661 gaactgattt cccatctgta agtattatag ctcctttatt ttttgcaact acaggtgcta
     9721 tactcatagc atcagcctct cctacaaatc catttgtaag cattacctct tttacactac
     9781 ttacttcatt tattttttta actacattat aactagtttc aattctattg ttaccattta
     9841 ttctttccac ttgaatacct tttcttttta tatctttttc tactgaattt tcaatagtat
     9901 tataaccacc aattatatat acttttttta caacatttaa actcttatta tctagtctat
     9961 ttaaagtagc ttttggtatt tcactttttt tagtcaaaag tattggtgcg ttaatagctc
    10021 cagctaatcc acttgatgaa agcccatcag ataaattttt atctccatta actaatatta
    10081 cagtatcata agtttgtcta tccgctataa gtcctgctgt ttcatatcta tcaattccta
    10141 ttattgaatc ttctttaatc tgtgcatttg cagttaaatt attaaaaaga aacatagtca
    10201 aaactaaaat taatatactt agttttttac tgaatttttt gaatttttta ttcataatat
    10261 acactattcc tcctgtaaat taaggtcttt aatttgctta taaattatcc ccttatttac
    10321 aaataataac ataatattat agtaacgtat atattacctt aaaattatca gtaactttat
    10381 atcaatcatc ttagcttatt tatataaatt tttttatatt acttttcaaa taacagtcat
    10441 actaaaacaa tttcatcata taatttgaaa attattacta tttacacagc ttttatcatc
    10501 atcttaccac catcaaatat atattcaacc atattattct catataaata agttatataa
    10561 gaacgtattg tacttccaac caacacatat tgattagcat caagcttgac attgtacaaa
    10621 tcacacattt tttcaataac ttcatcaatc ataactggct cataacaaac ctttttaata
    10681 ttatctatta tttcttccat cttcttctta ttaatattaa ttaaactttt tatatctgta
    10741 gttttgacag agtgacttgg aacaaagaaa tcagcctcta acattctcaa tttttctagt
    10801 gtcttaaact gtgaatctat atccagtagg aagaaaaaat gatatttagt aattatattc
    10861 tctggtgtaa gactatctgc aacaaacaaa acattatctg gagttctaac accaatcatt
    10921 tcaaaataat gacctggaag agaaatcact tcaagttctg tatctacaat tttgccattt
    10981 gaagggataa tataatcaac ttcactttcc tttgctaata aaaatttatt cttcagtgct
    11041 ttacttggat agccaccata taaaaatgct gattctaata ttggattttc tgtaaaatat
    11101 ccttcaattt tagtcgtagc aatcttgcat tttgtttgat tcttcaaata tgcatttcct
    11161 cctatatggt ctgcatttga atgagtgttt attattaatt taggatttaa attatgttct
    11221 tctaataatc ttaaaacttg tctagctgta tccttattat ttccactgtc aattaaaatt
    11281 gcatccttac catctacata tacacctact attgtaggga gtggaatata atatgtattt
    11341 ccttttatat gttctagagt taatttagcc attttataac ctccctttaa atttactttt
    11401 aattatagca ttaattttct gaaaattcca atcaaattgt ttttataata tatttttatt
    11461 tcttttatta aaaattggaa aaactatata atatatatta taatctatat ataaggagtg
    11521 atgttattga aaaaagatag aataactttt tcagaaattt taatattaat tgtaggctgt
    11581 atattaatgg ccatttctct taacttattt tttaatccac atgcaatcgc tgctggtggt
    11641 ataacaggac ttggagttgt tttaaattca ttattcggtg tagaactttg gattgtcaat
    11701 ttacttttaa atgttccatt attcatattt gcttacaaaa tactttcaaa aaaagattgt
    11761 tttaaaacgg ttttaggaat aatttttctt accatagcac ttaagcttac tgcaaatatg
    11821 gctacattag atataacaaa tgatatgtat cttgcaatta tatctggttc tattttaatg
    11881 ggagttggtc aaggacttat ttttagaata aatggctcta caggtggaac tgatttaatg
    11941 gcactcctac taaataaata ttttcctaca tttagtatac ctgttttaat gggtattgta
    12001 gactgcgttg tagttgtttt atcaggtata gtaaatagac aggttgaaat tgcactttat
    12061 tcaactgtgg ctttatacat tttagtaaaa gttagtgact tgcttattga aggatttaat
    12121 tattccaaat cattcactat tatatctgac ctttcaaaag atataagtaa aaaaataatg
    12181 gaagatttag atagaggtgc aactattcta aaaggagaag gtgcatatac tggagaaaat
    12241 aaaaatgtac ttttagtagt agttgaaaag aaagaagttg tcgaattaaa aaaattagta
    12301 aaaaatgtcg accctaatgc ttttattata attacagata tacatgaagc tttaggtaat
    12361 ggatttaaaa aaatagaata actaacaaat aaatagcaag tataattaac atatacaaat
    12421 tcaacttttt atatgttata ttatacttgc tatttatata ttatactttc tatttatata
    12481 ttataattgt tgtttttaag ataaaaatta aatgtatcaa ttattgtatt atacaagtat
    12541 ttccaaatcc gttctcattt agatagtatg cttagaaatt ttagaattat tgagtctaac
    12601 tttattactt acttcacttt ataacaatta aatacaaatt taaatattat aaaactacaa
    12661 aataatttaa agcatataat gtcaatatgt ttaaattata aaatattcat gtagttatat
    12721 aataaataat ttataaatat agccaactta gcctaaatac gtatataata tagctaatat
    12781 ggttcttata cttgatacca tcaataaaac taaaaatata aaaaggagaa cttaaaatga
    12841 gtgttagttt acaaaaagga caaagagtta gcttagtaaa agataataat cctgtaaaaa
    12901 atctagtagt aggcttagga tgggatatga ataaattggg taaaaaaaat tatgacctag
    12961 atgcatttgc aatagcattg actaaccaag ataaaatgaa tgttagaggt gacttggttt
    13021 attttggaaa tcttaaacac ccttctaaag ctattataca cactggagat aacttaactg
    13081 gaaaaggtga aggtgatgat gaacaaataa ttgttaattt agaagacata cctgaatatg
    13141 tacataaaat agtttttgga gtttctatat acaaagctaa aaaaagagac caggattttg
    13201 gtcaaatgaa caactctttt ataagattaa ttgattcaaa ttccaagcaa gaattattta
    13261 aatataatct tcaagaagac ttttctggta aagtaacagt tttggcagga gaaatttatc
    13321 gtagaaatga agaatggaaa tttaatgctt taggaattgg tcaaaatgaa gagcttcgtg
    13381 aattaattaa tacatataag taataattac aataaaaaat ttataatgag gagttggttt
    13441 aaatgggtat aactttagca aaaggacaaa aagtaagttt aacaaaaagt aaccctggtc
    13501 taaaaaaagt aatagtagga ctaggatggg atattaataa atatgatggt ggctttgatt
    13561 tcgatttaga tgcctctgca tttttaactg gaactgatgg aaaagtaacg aatgatggag
    13621 actttatatt ctataataat ttaaaacacg cttctggagc tgttgaatat atgggtgata
    13681 atagaacagg tgttggtgat ggtgatgatg aacaaataaa tgtagactta tctaaaatac
    13741 ctcaaaatat tgctaaaata tcatttagtg ttactataaa tgaagcaatt acaagaagac
    13801 aaaatttcgg acaagttgaa aattcatata taagaatata taatgaagaa actaatgagg
    13861 aacttattaa atatgaattg ggtgaagatt ttagcataga aacagcaata gttgtggcag
    13921 aactatatag acataatgga gagtggaaat ttaatgcttt aggttctgga tttgaggatg
    13981 gacttgcagg attgtgtaaa aattttggcg ttaatatagg ctaatatatc taaataaaaa
    14041 taagagtata ttgatatact cttattttta tttagatata ttataaactg tttacacttc
    14101 tactcttctt tctcgcttgt tttttcccat attctactga aatatcaaaa aatgctgaaa
    14161 tattttctgt aaaaaacttg cttctatgat atgcaatatc aaatgtccta tgtaaattaa
    14221 taccttcaat atcacaagcc cacaaaagat gcttttttaa ataatcctca ataagtaatt
    14281 cggatataac tgcaatgcca aaattatcta caactgctct taatatagct tcagagttat
    14341 aacatgacca tctaatattc attgggattt ttaattcttt taattgcttt tctaattgtg
    14401 ctcttgtacc acttcccaat tcacgcataa ttaatggttg atttgaaagt tctgaaactt
    14461 ttatagagtc tcttttataa aactcatgct tgtgtgaaca tataagtaca agattatcat
    14521 taataatgct ttttgtaaca atatctgggt gcttaatttt tccttctaca atcgcaatat
    14581 ctaattcact attaagtaat ttttttgcaa tcagtcttgt atcttctaca aatacttcag
    14641 gttctataag aggatttacc ttgtataatt ctaacattat tggacttatt acacatgtac
    14701 caacagttac tgttgcccca actcttatac ggatattttt tgcacaatgc ttcatacaat
    14761 cattcatttc ttcatttaca gctaaaagat gcctcccata tccaagaagt tttttccctg
    14821 cctcagttaa atataatttt tttgaaattc tatcaaatag cttaacacca tactcttttt
    14881 ctatttctga aatagcttgg ctaactgatg actgggtaat atacatattt cttgctactt
    14941 cactcatttt accacattca gcaaccttaa caaagatttc aagactacga attgtcatat
    15001 tatcacaacc ttagcaaaca ttagtaaatg cttatgattt tattaatatt ttagtatttt
    15061 acaaatatat tgtcaagaag tataatttta ttatagagtt ataaagtaga ttggagtgtg
    15121 atatattgtg aaagtattaa ttataggtgg agttgctgct ggaacaaaaa ctgctgcaaa
    15181 aataaaacgt gaaatgggtg ataattgctc tgttacaata ctaaataaag gtgatgatat
    15241 ttcttatgca ggttgtggtc ttccatatta cgttggtaag ataattgaag ataaatcatc
    15301 tttaatcgta aatacacctg aaagtttttc taaactaaca ggagcagaag ttcgttgtgg
    15361 agttgaagtt acatctattg atagaaatgc aaaacaagta actttgaaaa cgctttcaac
    15421 aggagaaaaa gaaaatctaa gctatgacaa acttgtaatt gctacagggg cagacccaat
    15481 aaaaccacca atacctggaa tagatttacc tggagtattc tttatgcgta ctccaaatga
    15541 tgctataaaa ctaagagatt ctatcgaaaa cggtattaag agagctgttg ttattggtgg
    15601 aggtttcatt ggtcttgaaa ttgctgaaaa tctttctgca atgggtatac gtgtaagtgt
    15661 tgttgatatg gctgaacatg ttatgcctgg atttgatact gattttgctg aatacgttga
    15721 aaatcatatg gctgataaag gcataatgat ttttacaggt gaccaggttg taggtattga
    15781 aggtgaaaat aaagttgaaa aacttcgtac taaaaataga gctattaaaa cagatttagt
    15841 ggttatgtca gttggaatcc gtcctaacac aggttttcta aaagacacag gtttagaatt
    15901 tgcccctaat aatacaatta ttgcagatga atatatgctt acaaatgaca aagatatata
    15961 cgtagttgga gactgtgctt ttgtaaaaaa ttctttaaca aataaaccta catggtcacc
    16021 tatggggtca tctgcaaatc atgagggtag aatttgtgca caaaatatat ctggaaaatc
    16081 taaaacttac aatggtgttc ttggaacaac agtagtaaaa ttacctgagc tcaatgcagg
    16141 aaaaacaggt ctaagtaaag aaatagctga aaaagaaggt tataatgttt gctctgtaac
    16201 catagctact gatgataaag ctcattatta tccaggtgca tctaatttta ttataagaat
    16261 ggttgctgaa aaaaatacta gaaaattatt aggtgttcaa gttatgggac ctggagcggt
    16321 tgataaaata gtagatatcg ctgctactgc aataacttta ggcgctgacc tttattcatt
    16381 agaaagtatg gaccttgcat atgcaccacc tttttcaaca gctattcacc cttttgtagt
    16441 ggcagtaaat gttttacaaa ataaacttaa tggagaactt gatagtgttt tacttgacga
    16501 aattgaggat tttgacagtt ggactaaact tgatgtttca aaagctcctt ctattcctag
    16561 tttacgttat attcctgttg gtgaaattaa tggagaaatt gaaggccttg ctaaggatga
    16621 aaaaattctt cttatatgtg ctaaaggtaa aaatagctat atggcgcaaa atcgcttaag
    16681 acgctttggc tatacaaata cgaaagttct tgaaggtgga atactatttt atccaaatct
    16741 taaggtaatg gaggagtaaa tatgagtaat gttacagcag aacaaaaaaa ggaagtaaaa
    16801 ggtagaggtt ttctaccaag cagggatgga gaacattttg cagcacgtat aataactgta
    16861 aatggtgtaa taaatgcatc acaagcaaaa aaaattgcag aagctgctga aaaattcgga
    16921 aacggtcagt tagcatttac tactagacta acagtagaat tacctggtgt aaaatttgaa
    16981 gatattgaag ctctaagtga atttattgct agcgaaaatc taataacagg tggaactgga
    17041 tcaagggttc gtcctgttgt ggcttgtaaa ggtactgttt gtgttcatgg tctagctgat
    17101 acacaggcac ttgctgctga aatacacgaa gaattttaca aaaattggta tgatgtaaaa
    17161 ctacctcata agttcaaaat tggtgttggt ggatgcccta ataactgtat taaacctgac
    17221 ctaaacgatc ttggaattgt tggtcaacgt gtacctgact atgattcaga gctttgtgtt
    17281 ggttgtaaaa aatgtgctgt tgtagaggtc tgtcctgtta aagcagctaa attaacagat
    17341 aaaggtaaac ttgaaataga tagtaatcta tgtaataact gtggtaagtg tatagaaagt
    17401 tgtaattttg atagtattga agaaaaagaa tctggttata aagtctatat aggagggaaa
    17461 tggggaaaat cagttcgtcc tggaacacag attgatagat tattttcaaa agaggaactt
    17521 atgactttaa ttgaaaaagc aattctttta tttagagaac agggtaacac aggtgaacgt
    17581 tttggaataa ctatcgatag aatcggtgct gataaattta tagaaatgct tttatcagat
    17641 gaagttttgg aaagaaaaga agagatacta gaggctcttt tacaccttac aggtggagct
    17701 gtatgctaac aataaatatt ttaattgtac agataaccta tggaaaaaat tttagttaag
    17761 aataattaaa attctatata tgatatatga tatatgaggc tatctcaaaa cacagatacg
    17821 cctctttttc attgttgaaa tgataaagta tatttgagtt catataaaaa agagtgtctc
    17881 gtgaattata aaattcattt tgaggcaccc acattatttt ataataattg cttattaatt
    17941 ttattttaac tatataaaat gtacaaaaag taagaatatc caatattcta aaattaattt
    18001 tgaattgtat tgattgaaaa gtttactatt ccactatttc catcttgatt tactactaga
    18061 taccatgttc cagtattagg aactctcatt ctacatggat ttttctttgc aaatccacca
    18121 tagtaatcat aatttctttg atttttaaat ttcacataat ttatatggtc taacaatatt
    18181 acattggctt gtttctctaa atttacctct acaatatccc ctttttttaa gttcatctta
    18241 ctataactat attccattga attactcctc cagtatcgaa taatctttgt tatttactca
    18301 tctcaaatat gtaaaaattc atttaaaata ttacaaatac tatataatta gactattatc
    18361 cagttttaac cttatttact acaatccaaa ggttattaaa aatattattg gcaacaactt
    18421 ctccatccca tacaactctt tcttctttat tttctaataa agcatagtac ccagagtcag
    18481 aactttcatc tttatagact gtaacataaa actcttcatc actatattca ggtatatttt
    18541 taaagtcttc agatatttta catgttaata tataaccttc taattcttca tctataaaca
    18601 aagcaattcc tccaatct
//
LOCUS       DAGSWI010000078        18549 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.078, whole genome shotgun sequence.
ACCESSION   DAGSWI010000078 DAGSWI010000000
VERSION     DAGSWI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18549
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.078"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(490..1362)
                     /locus_tag="MJF80_002432"
     CDS             complement(490..1362)
                     /locus_tag="MJF80_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBZ0281088.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(1756..2334)
                     /locus_tag="MJF80_002433"
     CDS             complement(1756..2334)
                     /locus_tag="MJF80_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBZ0281089.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(2392..3108)
                     /locus_tag="MJF80_002434"
     CDS             complement(2392..3108)
                     /locus_tag="MJF80_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBZ0281090.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            3299..4384
                     /locus_tag="MJF80_002435"
     CDS             3299..4384
                     /locus_tag="MJF80_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBZ0281091.1"
                     /translation="MIIISRENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIDFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(4539..5198)
                     /locus_tag="MJF80_002436"
     CDS             complement(4539..5198)
                     /locus_tag="MJF80_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBZ0281092.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(5415..6707)
                     /gene="lysA"
                     /locus_tag="MJF80_002437"
     CDS             complement(5415..6707)
                     /gene="lysA"
                     /locus_tag="MJF80_002437"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBZ0281093.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(6725..7918)
                     /locus_tag="MJF80_002438"
     CDS             complement(6725..7918)
                     /locus_tag="MJF80_002438"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBZ0281094.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(8279..8896)
                     /locus_tag="MJF80_002439"
     CDS             complement(8279..8896)
                     /locus_tag="MJF80_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281095.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(9066..10766)
                     /locus_tag="MJF80_002440"
     CDS             complement(9066..10766)
                     /locus_tag="MJF80_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281096.1"
                     /translation="MKRYLKTYGVALIIVIIYSFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(11163..11726)
                     /locus_tag="MJF80_002441"
     CDS             complement(11163..11726)
                     /locus_tag="MJF80_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281097.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(11885..12598)
                     /locus_tag="MJF80_002442"
     CDS             complement(11885..12598)
                     /locus_tag="MJF80_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBZ0281098.1"
                     /translation="MGKVAKILLSLIVIVLVLTGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            12890..14554
                     /locus_tag="MJF80_002443"
     CDS             12890..14554
                     /locus_tag="MJF80_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBZ0281099.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(14661..15560)
                     /locus_tag="MJF80_002444"
     CDS             complement(14661..15560)
                     /locus_tag="MJF80_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBZ0281100.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(15587..16225)
                     /locus_tag="MJF80_002445"
     CDS             complement(15587..16225)
                     /locus_tag="MJF80_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBZ0281101.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(16562..17560)
                     /locus_tag="MJF80_002446"
     CDS             complement(16562..17560)
                     /locus_tag="MJF80_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBZ0281102.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFITSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(17658..18233)
                     /locus_tag="MJF80_002447"
     CDS             complement(17658..18233)
                     /locus_tag="MJF80_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281103.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
BASE COUNT         6000 a         3062 c         1966 g         7521 t
ORIGIN      
        1 tttattttta taaagtatta tatttctaaa aaagtttccc tttccccaaa aatggctact
       61 taaaaaatca agatttcata cctaactact aattctatga tttttaaatt tttcaacttc
      121 tttgtcaaag ttaaaataaa atactaaaag acacatatta tagtgaacaa aaaagataga
      181 aatacatatt ttaatataat tttaataaga tattatatac ttaaatacaa ataaaattaa
      241 ttttataatt gttaattaag gaaatgatat tgtttagaga aataaaaaaa gcactctcca
      301 tgaatggaaa tgctttcttt aataaattag ttgacgtagt aaagatgtat tcagaataca
      361 tctttatgtt ttgagtctcg aaatatttat ttgtattatt taaacgaaaa tttatttttt
      421 aagtgataat cgagtattta ctaataaggc tatgattctc aatttcatag ccttattaat
      481 atacaaactt tactcataca cagaatatct tttccaaatt tgctctaaat catcaaaatg
      541 ctgtttaata tcttcttttt ccttcatccc aacagatata gcaagggcat ttgaaatact
      601 aagagctgga actagagaat ctacaaatga agccatatta ctcttaacta gaagagtgtt
      661 atctgcaagt gatgctactg gcgcaaataa gctatctgtt aatgatatta catgggctcc
      721 tttttcctta gcataattga caatttgata agatttctta gaataacgag ggaaactaat
      781 agcaactatt acatcttctt catttattct tacaatctgc tcaaaagcat ctcccatatc
      841 cattctaatt atatgaacat tgtcaagtat tatatctaag taaaaaccta aatattgagc
      901 tactacaaaa gaactcctca ttccaagtat atatattttt ctcgctctta aaagtttgtt
      961 agaagcttct ttaaacgctc tctcgtcaat ttcttctagt gtagacctta tattatctat
     1021 atcacttttt aaaactttgt ttagtattgc aaaatcatcg gaataatcat gtgccatttc
     1081 aactctttgt actgtagtta gtttattttt tatgagttct tgaagtgcag cttgtaattt
     1141 aggatatcca ctataaccta atgcatttgc aaatcttaca actgtagact cacttactcc
     1201 tacagtttca cccaatttac atgcagtcat aaatgcaact ttatcataat tatttaaaat
     1261 gtattgtgct ataagttttt gacccttact caatctcgta tattgagatt gtatatttga
     1321 tatcaaatgt ttactgtcct tcatttcatt tgtttcttcc atttttttct ccttttagtt
     1381 atataaacta ttttgtttta ttaacactgt ctttttctct aaaaatttta ttttttatct
     1441 caatttcatc ttagttttta tcatagcttc ttaaactcat aaaattctaa cttttctgta
     1501 ccttatactt cacatagtat tttcgattaa atttattata ccatctttcc ttgcattttt
     1561 taatatttat ccaatatttc ttttaaattt ttcaattttt actcatttaa aagttatcta
     1621 tcttattttg catatttaga tgtatatttt gttttgagaa taagctgttg tatacttatt
     1681 atttaaagta tttttaatag gtcacactat atatattaac tcttaataca atcttgtcaa
     1741 gttttttagt atttattatg cttttttctt ttcaattaaa atcaattgtg gtgggttatt
     1801 gacttgattt aaaaacttag attctaaaac attaaattca tcttgattta aattattgac
     1861 aaggttatat atgcaatctt tttcttcttg acctccatca tgccctgtat atatggcaat
     1921 gcttattact ccattttctt ttagcatttc taaacttaac tttatagcct ttaaagttgt
     1981 atctggtttg gttataattg aatgtttcgc tcttggaaga taacccaaat taaataaaac
     2041 acatgaaact tcttctttta tatattgatt cattttttca tgcccatcaa atatcaattt
     2101 tactctatct gtatatcctt ctttctctag tttctttctt gtagacttta ttgcttcttc
     2161 ttgtatatca aaagcatata caaagccctt ttctccaacc ttctcagcta gataccttgt
     2221 atcataccca tttcccatag tagcatcaat tactatgtca ccttcattta taatattttc
     2281 taaataaaat ttatttatat ctgtaatttt agttaaatac tttgcatatg ccataatact
     2341 atttctccct actattaact ttatttcatt ttttctcaat ttatttttat cttattttat
     2401 tgattttaaa tattttattt catcttctgt taggtatcta tattctccaa ctttggtgtc
     2461 ttttaatact attttcccca cagaaatcct tcttaaattt aaaactggat gatttatagc
     2521 tttgcacatt tttcttactt gtctatttct accctcatgt atagttattt cacacactga
     2581 ataatttttt tctttatttt ctttagttac ccttattttg gcaggtgccg ttgtgtaatc
     2641 ttctattttt agtcctgttt cgaattgtct tatctcatca tcacttataa ttcccttcac
     2701 acttgccaca tacgttttat caatttcatg ctttggatgg gtaagcttat atgttaaatc
     2761 gccatcatta gtaagtatta ataatccact agtttcataa tctaatctcc ctattggata
     2821 tactctttcc cttacatctt taagtatgtc tattacactg ggtctattga attggtcttt
     2881 tactgtagtt atatatccct ctggtttatt taatactata tatatataat tttcgcttgg
     2941 ttttactttt ttattatcaa attctactat gtctttttct tcatcaatat taaaagataa
     3001 ctcttttact acacttccat ttacctttat tcttcctcca agaattattt cttctgcttt
     3061 tcttcttgat gctattccac aagaagctat atacttatta attctcatac ttaatccttc
     3121 cttatcttaa attatgtgtg tttaatattt ttatcaatgt taattttgct acattattat
     3181 actatactag accttaatta aaaacaagca aaaaaattat ttaattttcc taaataattg
     3241 taatttcttt tcacatacta ttaatatgta atagctgact aaacactatt ttaaggaagt
     3301 gattattatt tcaagggaaa atataaaata ctatttaata gtcgtgttta ttggaatttt
     3361 attttttaaa tttattaata ctccatcaga ctttatatct agtattgaag gattcttaaa
     3421 gttttttagc ccatttttat tagctatttt acttgcttta cttttaaatc cacttgtgat
     3481 gctttttgag gtaaaattca aagcacatag acttttagcc atttttttat cttatatatt
     3541 tataggtttt attttagcct ttgccattag gttattaatt ccatcaatag ctaatacatt
     3601 aaatagattg ataaatgaaa tgcctatgta tactgattat atagatagtt ttatagaaaa
     3661 aaatatgtca aacatagatt ttctaaaaac tttaattcct catatacaac atagtttaga
     3721 taatttatta aaagaagcaa gtaattttgt tggtaaaatt cctaaaaact tcttaatcta
     3781 tacacttagt atatcttcta tgctatttaa tatgacaatg ggatttatac tatctatata
     3841 cattatatat gacaaagaaa aaattgcatt gggatttaag agatttctat attcttctac
     3901 tgctagaaac aaggcagata acattataga attttttagg actactcatg atattttcta
     3961 tgattattta cttggtagaa tattagactc tcttattatt ggtataattg catttttagg
     4021 atttcaattt gttatcagaa tagaaaatgc tttattttta gcttcaataa tattcttagg
     4081 taatataatt ccctattttg gtccttttat tggtgcaatc cctccaatag ccatgactat
     4141 attatatagt ccacaaaaaa ctatatgggt tattgcattt ctatttatat tgcaacaatt
     4201 agatggaaat tttatagaac caaaagttat gggtaatcaa gttggtatag gagctatatg
     4261 ggtaatatct gctattttgg tgggacaatc tctatttggt ttcataggag tttttctttc
     4321 agtaccaata gctgctgttg taaaaactta tgttgataaa tatatagata atcgtttaca
     4381 atagcttatc tctactgtga ctttaatttt tagtttaaaa tttaactgct atttattaaa
     4441 ttatttctca aaaaatatct cttagtaaga aaaaattgct aagagatatt ttttataata
     4501 tataatttgt tcattaaatt tatgcatata ttttatactt attgattatt tgagttttga
     4561 gaatttccac cactatctga accaccagta gtatcttgtg gagttggtgc tggtgttgga
     4621 ttactattat ttggcttttc ttgattttga gaagaactgt tattattgtc agacttatta
     4681 ctatctgatt ttttatcatt atttttagaa ctagatttat tatttttact acttccacta
     4741 taagaagatt tccttgcatc tgattcaact ctatacctat cttctccagc cgtatctttt
     4801 gcctttttct ttctttgttc ctctgcttcg ttttgttctt tttctaattt agttaactct
     4861 tctttctttt tagttgcctc ttctttaata acagaggaac catttttgat tttctctata
     4921 agtttaagtt ccttgattgc accctcataa ttttttaatt cttcaaactc cgtcatattt
     4981 gacattctca tagcttgcat atacatagct cttgcattct cattagtact atcttcttcc
     5041 agtgcagagg ataatgattc catagctttt ttatagttat gtttttctaa atatgttttt
     5101 ccttgctcta ctaaatttat ttcatctttt ttattaaatg aacatcctac aagaaatata
     5161 gataatatcg atattaataa aattactctt cctctcatta aatccctcct tgtcatatac
     5221 tttaaatttt atcataatta taaagttttt aaaatatttt atatttatta caagaaattt
     5281 gttagtaaaa caaaaaataa gcagatatgc tacgaacgat acaataaacc aaaaattaat
     5341 ttagagatac atatcctagc aaatctgctc ataataaact ttaatattta atttttatta
     5401 tatacttctc tattttatat ctctaaagct aataagtcct catagctttg tctcttacaa
     5461 attatcttat catttccatc aaatactgat accactgctg ctcttggtat tttattataa
     5521 tttgaagaca ttgaatatcc atatgcacca gttgatgttg ttataagtat atctccactt
     5581 ttaatatcca ttatgtttat atctccaatt aatatatctc cactctcaca acattttcca
     5641 gctattgtta catgattcat agcctcatga ttaattttat tcacaatact acactcataa
     5701 ctagcttggt acaacgaagg tcttacatta tctgtcatac ctccatctac acttacataa
     5761 accctaacat cttttatatc ttttatagaa cctactgtat acaatgtact tcctgcattt
     5821 gcaactattg atcttcctgg ttctattacc aatgtaggta cttctattcc aagctcttta
     5881 catgaagttt ctgctttagt gattatggtt tcacaaaact cttttatagt tttaggttta
     5941 tctccttcgc tataataaac gccaactcct ccacctaggt caatttctgt tagttgtatg
     6001 ttgaattttt cttttatttc ttttaccaag ttcatcatta tatccactgt atctatgtat
     6061 ggttctacat caaatatttg tgaacctata tgagcgtgaa gacctacaag tttaatattt
     6121 ttatattcac ttaatttttc tattgctcta aataaatctc catttactaa agcaaatcca
     6181 aatttagaat ctatctgtcc agtttttata taatcatgtg tatgagcttc tataccagga
     6241 gttattctaa agtatatctc ttgagtttta cctttatctt cacataatgc ttcaagcaaa
     6301 tctaattcat aaaaattatc cactacaaat ttacctacac ctaaatctaa tcccatgttt
     6361 atttcttctt tagtcttatt gtttccatga aagaaaactt tttccatagg aaaatttgat
     6421 ttataagctg tatataactc gcctcctgaa actacatcta aacacatttg ttcttcattt
     6481 ataagattac acatgtaaag aggtaaaaaa gctttccctg cataagcaac cttatttttt
     6541 ccctctctag ccttaaaata ttctatatat tctctacaat tttgtctaac taaagcttca
     6601 tcaaatacat aaagtggagt ttgatatttt ttactaagtt ctaaacaact cactcctcca
     6661 atatataatt catcattatt tacagtcata gttccatgaa gtttcataat agcctcccca
     6721 aattttatat attaaatact tctgctattt tttctatagc tacttgctta tcatcagaat
     6781 ttatagcaca agttatacga atctctgatg tagttataag ttttatcatt ataccatttt
     6841 cattgaatat cttaaatact ttagcagcaa ctccagaagt atttttcata ccaagaccta
     6901 ctaaagaaaa tttagtgata ttattatcaa tcactacatg ttcttcatct gtatatttag
     6961 atactatctt tttacattct cccaactctt cttttggaac tgtaaatgat acacttatct
     7021 tatctaaaat aggtgcagtt tggcttatca tatctacact tatacctata gttgctatat
     7081 cttcaaataa gctagatata ttttcagctt taaaatcttt tattgttata gaactgtcat
     7141 catcactagt agctaatcct gttattactt tatcctctaa tttcatgtct tttccccctc
     7201 ttatatatgt tcctttttct gtaccacatg tacgtccgac atagatttca acaccatatt
     7261 tttgtgcaag ctctatactt cttgaatgca ttacttgtgc tcctaaactc gctaactcaa
     7321 gcatttcttc atattcaatc tcatcaagtt tactagcctt tgaatatttt cttgggtctg
     7381 taaaataaat tccatcaaca tctgtatata tctcacattt tccatttaat ttaaccgcaa
     7441 gagctacagc agatgtatct gaaccacctc ttccaagagt ggttacatca ccatcttcgt
     7501 tgattccctg aaatccagtt actatcacga catttccttc atctaaacta tttttaattc
     7561 tgtttacgtt tatatcatca atttgagact ttccatgaag accactcgtt tttatattta
     7621 actgataagc attataactt atagctttac atccaagtgc atttaatgct attgataata
     7681 aagatgctga aatcatttca ccagtagaca ttaaagcatc taattccctc ttactaggtt
     7741 cattactcaa ttcctttgct aaagttatat attcatcagt ggattttccc atggcagaca
     7801 caactattac catttgtgga ttttcttttc ttcgttctat tatattttct gctattgatt
     7861 ttattttttc agtatctgct actgaacttc caccatattt ttgaacaatt atactcatgc
     7921 tttctccccc tcaagctaaa ttttgataaa taaaaacgcc caaaggtata ctttggacgt
     7981 gtaaatttca tagaccaagt aaccttgtag ctcaccactt taaaagtgac agccttatgc
     8041 atattctgca taagtccaga aaatttgtcc gtcaacaaaa tttcttcggc tatataccct
     8101 ttcatcaatt atcaacgctt accagctcct attgaatact attgaataca gctacctcta
     8161 ccctaggctc attattttta attaatttat taatatagtt ttatcatatt ctatataata
     8221 ttgcaacaat tattttacaa aatgtggcaa atcgatagtt ttcttttcta aagttagctt
     8281 atatacaatt aaatagttta tttaatccta aatctataaa atttatgcta tactcattat
     8341 catatctctc taaatcattt aattcggata gattatctgg tgtaactacc aaaacgtttg
     8401 gtactttaaa aaatggaatt gtagatttat ctattttttt agaaagattt tcgtatttag
     8461 aaatgtctat ttcattattt aagtcaacat caactaaaac ttgctttata ttaccaaatt
     8521 ggtctgtata ttcaataaat aaatcgtatt tcacatcatc tacactaaca tttcggtaaa
     8581 atctttttac atcacaacct gcagtattga ttctaacaac taattctgat cctactagag
     8641 cctttattaa atcatctccc ttaaaaagtt tactaccgtt taaatagtat acataagaga
     8701 ggtctttata attataataa tccacatcac aaggatattt ttttagtgta tattcactac
     8761 acatttttct caaagttttt ttaaaatttt ttttattgta tcttttttgg tctccataaa
     8821 tttttctaaa ttgagactct gtaacacatc taaccctgct aattaaaagt aatatctctt
     8881 tttccatatt cgtcatagta cattctcctt gaagtattat ttgcaaagtt gtactaatat
     8941 tatattaatt ttatgacatt aaatttacaa agaattaaaa taaaatagct aatttttata
     9001 gaattaaatt ctataaaaat tagctatcac tatgttaagt actattatca atctttttat
     9061 ttaaattata cattaccttg attttcagaa tctttggaat ctgtattttc atcagttcct
     9121 gttttttctt ttgctttttt atcttcaaag tttgcaatat ttaggttatc aaattctgac
     9181 atttctatta caccagattc ttcttttgaa tacgatattt tgactttatc accaacctga
     9241 gtcaatggca attcgctaga tacttttgaa gtagccctaa atattatatc tttatttgaa
     9301 tctaaagtta cataataatt agtgttacca cctcgcacat ctgggcttat tcttgtcact
     9361 gtgccttcta atacctcatc ttttaaatca ttatcaagtt ttaaatcatt acccttagat
     9421 tctaatgcat ccttatagtt tcttaatgct tcattcttat cttcacctat tccaagtaca
     9481 ttataatctt caacagatac aaatgcaact ctcttaacaa gaccagcctt atcttttaat
     9541 gatgatacat atgttggctg acccaatata ttatacatta cagggaacgt agcttcataa
     9601 tttttttctt gaacattacc ttcagctgat ttcattgcag ccgtttcagt agctcctggt
     9661 tgtttatata atctagcttc ttttgttcta gaatcaacta acataaatcc aattgtagat
     9721 tcatctccac ctgaagatgt tatacctgta taccaataag acctgttatc attgccataa
     9781 actaatgatg taccttctgt tggtactaaa actccctttt ctgatataac agagtttaaa
     9841 aatccattaa cataaacacc ccagtcttta atctggtctg ttacaaagct ttgtggttgt
     9901 attctatcca cccatttagg tgtgttttta acatcataaa catttatttt tccagtttct
     9961 gcatcaacag tggctattcc tgttgcattt gctcctccat agccaatttt atgttcatat
    10021 aaacttacaa cccaataagg tcttccttca tcatttattt caagagtgaa atctgtcata
    10081 ccaacattta caataccatg tatgtataaa tgtctttgta aatcctgatg taagtaggct
    10141 tctggttggt aaactatctt tattggcttt ccatctattt cttgaactaa ttggacgtct
    10201 tgaggattac tagctgatac ttttacataa ccacttgtac cacttagaga tgttatccat
    10261 ttaattatgt ctctgtgaac aagaggcgct acccagtaca actctccatc tacattttgt
    10321 atatgaaatt ttcctaattt ggatacactc cctattgcag gaacttctcc aagctttttg
    10381 tcacctaatt tcattgccat atcttcatct acaagacgaa tgtcatttac actgacaggg
    10441 ctaatgtcat cagtaaattt actttcagtt acttttccca atagttctct atatgatttt
    10501 gcatgaagta caggtgtaga agttataaag ggaactacta caatatacac aagcaaagct
    10561 attgctattg aaaaattata tttagccatt ttagatgctc tttcatttct atctaacatc
    10621 atatctagta ttcctgctac aacaaagaaa attattccta ctgaaaataa agatgtaaaa
    10681 tctaatctta gtacaggtaa tttaataaat gaataaatta ttacaattat taatgcaact
    10741 ccatatgttt taagatatcg tttcatttaa tactcctctc taaaaaatgt tattattact
    10801 attatatcca tatatttaca aaaaataaat atttatcata tttattatat attaagatat
    10861 ctttatatat ttatagttct gcttattcaa ctatatttaa cattatacgt gtatgtataa
    10921 atacttttta tacactgttt gtgctatcca tcccgcacct aaaggagtgg gatttttgca
    10981 ctattctgta ttatatatat ccaaaaatat tataaaaata caaatattag cagaaattta
    11041 aaaactgaag aaagtattct aactaagatt tacaaattct taatcctatc gaagttaaac
    11101 tactatcttc agcctaatta tctagcaata tttttttata tgaaaaactc taaatcaagt
    11161 ttttaaatgt ttgcgatttt tgatacataa gaatgttgtc tagaattata ttctgataat
    11221 aaaccttcat actctaaggt taagccagat aaattataaa aatcatttgg cgttacttca
    11281 aatgcttgtt tatttaaaat gtctatctca cttttttcta acatgtcagc aacaaaattt
    11341 gaacatacat atttatattc agattgtctc tgaatattca ttagtaaata tattaatgcc
    11401 attgtatcgt aatagtaatc ttctctataa tcactaatta attttatatt tttatataga
    11461 ttttcatatt gtaaattatc tacttctaac gaatataccc tacacacagt gttttttctt
    11521 atagcatata atccttgatt tatattttct tcaacaaatc ctccaataaa tggattcctt
    11581 ggatttagcc ttccaaaaga atacattggt tttagatttt catttaatga tattgacaca
    11641 tgtgtataca gctttttact tatattcctt attaagtaag ataatatagt tcctgtatat
    11701 gttgttacaa tatagacttt attcatcatt tccccccaat ctagtaacta aataatatta
    11761 tacacttaca atttaatatc ataagatatt ctaacataat aaatgtggtt ttacaaattt
    11821 tgattttaag gtactttaat attatataat ataataaaaa tataaatata agttttctat
    11881 atttttaacc aacagatgtt ttcttcaaat taatcagatg atttagtata tcttgaacct
    11941 ttccagagtc ctttaaatat cctaatgctc catttatttg tgattttaaa tctatttcta
    12001 cttccatatc caaatcaaca tttccttttt ttatagatat atttttaagt gttattgggt
    12061 aaagataact tttttccaaa gtaatcttat ttccattaac ctcaaatgga acttttttat
    12121 tgtagttact taatattttt tctagcattt tatcacttag tttaaatttt ccaattttca
    12181 cattctcaag acttacaact aagtttccat cctttaaagt aggtctcaat tcaagttcat
    12241 attgactatt taccaaacca aataccttat atggtcctgc tacctttatt ttaccatttt
    12301 tcatttcaac aaaattattt tcaagttcat taattccatg cttctgcatc aatgtatata
    12361 ttaaattttt aaactcttcc tctgaaacag atatttttcc aaccattcta agtggatttt
    12421 ttactacctc catagagttt atatatgagc cttttgataa atattcttca tctatgattt
    12481 tattattttg tgaactaaca tcatatcttt cttttggtgt caagatatat acaacaattc
    12541 ctgtaagtac taaaacaata acaattaaac ttaacaaaat ctttgcaacc tttcccataa
    12601 tcgtctcctt tacaaatctg tcatcagatt taaaataaat ttagataact aacttcacat
    12661 aatattctta acaatataca tactataata cagtctttta aaaattaatt caaccaatct
    12721 aatgttttgt aaataaactg taaaaacatg tttttagtat aaaagttaat attttgcata
    12781 atactcttaa ttacaccttt atttttttct aatcttggat taaaattaat attatgcaat
    12841 acagtgtagt gttctgtatt ataatcaaat taaataggag gtatagaata tgtcaaatga
    12901 aacgaactca tctaacttta taaagaatat tattataaat gaccttgaaa caggaaaaca
    12961 tgacagtatc ataactcgtt ttccacctga gccaaacgga tatttacata ttggtcatgc
    13021 aaaaagtata tgtcttaatt ttggattagc taaggagttt aatggaaaag ctaatttaag
    13081 atttgatgat acaaacccat taaaagagga tgttgaatat gtagagtcca taaaagaaga
    13141 tgtaaagtgg ttaggatttg attggaacga gttaaatttt gcatctaact actttgatga
    13201 aatgtataag agagctttaa tactaataaa aaagggtaaa gcttatgtat gtgatttgac
    13261 acaagaggaa atgagagaat atcgtggtac tctaactgaa ccaggaaaag aaagtcctca
    13321 tagaaacaga actattgaag aaaaccttga tttatttgaa agaatgaaaa atggtgaatt
    13381 taaagatggt gaaaaaacat taagagccaa aatagatatg tcttctccaa atataaacct
    13441 tagagaccca attatatata gaatatctca ttcaacacac cataatacag gagataaatg
    13501 gtgcatatat cctatgtatg cttttgctca tccaattgaa gatgcaattg aaggaataac
    13561 tcactctata tgtactttag agtttgaaga ccaaagacct ctttatgatt ggtttgttaa
    13621 agaatgtgag atggaaaata tacctagaca aatagaattt gctagactta atataaataa
    13681 tactgtcatg agtaaaagga aattaaaaca acttgtagat gagggtattg ttgatggttg
    13741 ggatgaccct cgtgttccta caatatccgg aatcagacgt aagggatata ctgcagaagc
    13801 attgcgcaat ttctgtagtg aaataggggt atcaaaagtt aactctacgg tagatagtca
    13861 aatgcttgat tatttcctaa gagaaaactt acaaccaaag gctcctctta ctatgggagt
    13921 tttaagacct ttaaaactta ttataactaa ttacccagaa gataagattg agatgcttga
    13981 aatagagaat aatgctaaag atgaatctca aggaaagaga ttagttcctt tctctagaga
    14041 attgtatata gaacaagatg attttatgga agaacctgtt aagaaatatt ttagattttt
    14101 cccaggaaat gaagtccgtt taaaaggtgc ttattttgta aaatgtactg atgtaattaa
    14161 ggatgaaaat ggaaatgtag ttgaaataca tggtacttat gaccctgaaa ctaagagtgg
    14221 ctctggcttt acaggacgta aggtaaaatc tacaatacat tgggttgatg ctaaatcagc
    14281 tataccatgt gaatttagat tatttgaacc attaattctt gatgatatac ctgaaaatga
    14341 aggtaaacat ttcttagaac aaataaatcc taattcattg gaaatattac aaggttttgt
    14401 tgaacctaca cagataaaag atgctaaacc ttttgataaa ttccagtttg ttagaaatgg
    14461 attctttagt atagataata aatatacaac tgatgaaaaa tttgtattta atagaatagt
    14521 tcctctaaaa agttctttta aaccaggtaa ataatcttac aatcaaaaaa tgaagactta
    14581 aaatataaat tacttctact ataaataaac ttagttagaa gtaatttttt atttgagtct
    14641 tcatcattta ctttaaatga ttaacttttt tcactacaga ttcccatgac ttctattcca
    14701 tcttttatgc attctatgtt aagaggaaaa tctggtcctt tttctccgtc aatatctgtt
    14761 ataagtgaat cttcacattc tatatgcacc tttttagttt taaagtatag tatttcattt
    14821 ccattatatt gatctaaata atcacctcta agcacactta tcaaaactgg tattgatttt
    14881 ggtattggca ttcctttaaa aataattaca tccaaatatc catcatctat ttcagcctta
    14941 tatgctagat ttatatttcc tgctgttttg ccattaaaaa tcaacataag atacatatct
    15001 ccatcataat acatttcctc agaagatact tttatcttaa atttcctcat atacaatgct
    15061 tcttctattc cctttatata ataagaaatt cttccaaatg aattttttaa gtctgtgttt
    15121 atcctttgtg atacatccgt aaacatacca gcacttgcaa catttataaa gtacttatta
    15181 ttgactttcc ctatgtctat tttctttggt tttgaattta tgatttcatt tatagcttct
    15241 tttatatcaa atgaaatttg aagagcattt gaaaaatcat ttgcagttcc tgtaggcaaa
    15301 attccaattg gaatttttat gtctaattct ttcattgcat ttactactaa atctacagta
    15361 ccatcaccac cagctatcaa tatgtgttca taagaactat caacttcctt aaatgcattc
    15421 tttacaggtt ctttaatagt taatctatag ggtatcaata aataattttt ttcttgatat
    15481 aattctatga tagtgtctaa atttgataat atcttttttt cacctgaatt aggattgtat
    15541 ataagtttta cttttttcat tatttctcct tactcatttt attcttttag tatccaaaat
    15601 cttcatttaa aaaaatcaca tgtattcttt ctggtgttaa ttgtcttttt ataagtgtat
    15661 aactacagca tattcttcct ggacttgaac agtctataca tataccagta gtcttacatc
    15721 cagtatttgt acttaatctt ttacaattgg ctggagcagc tattgttttt gttcttttta
    15781 tagcttcatc tacatcttta actattttat ttactcctgc tataactatt actttatcag
    15841 gtccatatat catagcagca accctattcc cattcccatc taaattgtaa agttctccat
    15901 cctctgttat cgcattagta cttgtaaaat aagcatctgc agaaaatgac tttctatata
    15961 tttctttcat ttcttctggt ttgatatctt ttgcatatct atcaagaaaa ttatattttt
    16021 ctcttataaa atcaatcact cctgtttcaa ataaggtcat agaacctcca catcctacta
    16081 gagaacctgc tttaactatt tcttcaattt ttttaagaag ctcttctttg ttacttacta
    16141 aatacccttt catattattg ttttccaaag attttattgt tttttctatt cttttttcat
    16201 taagccaatt taaattttga tccattttaa cttccccctt atttttaata tgtacttaat
    16261 tatttataaa ctttattttc tttataagtt tattttagta catctaaaaa aaaatacaat
    16321 ataaaactta aatagtttta cataattttt tccaaaataa cttactcaat caaaaaagga
    16381 aagtagatgt actattctac tttccctata ttgtatgtag tatttattgc ctaaaatcta
    16441 tatattaatt atatgtttca tattatataa tatcacagtt aattaaaatg taatactatc
    16501 ttctttttaa acatataaat tgtattatta aatttaatat atccaacttc aataaatctt
    16561 attaatttga tgaagaagat gctatagctg cacttgttgc tgccattaca catgcaattt
    16621 gaatagcaac aacaatattt aaaaatatat tattagttat agagcctata ttattctcat
    16681 ttcgtatcaa atctgcataa attgaagcta caatagcaga actaatcatt actctattac
    16741 tttttcctaa agaccaattt ccaaagcctt tttcttcttt aagtttattt gaaacatttt
    16801 ttatctcttc tattatttca tcttctctat aatctaataa tgaaattgct cctattattg
    16861 gatatccata ataattcatt ttacaatcat tttctttaaa tgctttttta agttcaatag
    16921 tctttttaca tttaagcata gttctatcat cactaaataa aagaatttgg gataatgctt
    16981 gtaaatcatt tcctttataa aaaccatttt catttagaaa ttcataacat ttttctatgt
    17041 attctaaatc ttcatctaaa ttttttgaat gaattgcaat aagtgctata ttacaatagt
    17101 catcactaga agttataaat ggatgttttt tcctcatgta atcatatatt attttcattt
    17161 ttgatataca tgtaagataa tccattttct ctctattttc ataaactatt gatgctgtaa
    17221 gtggtaaata tgtatcgccc caaaactttt cacttttaag ttctttgtat atatctaaaa
    17281 tcatcttaaa actactttct ggatttgatt caaacgaaag tagtgttgcc atataaaaca
    17341 tattatatcc tctaaaatta gagaataatc cagtattttt tttaataagc tctattcgtt
    17401 cttccactaa tctaggactt attctcttgt tcttaagggt aaatgcaagt gcacaactat
    17461 gttgtatcaa tcccatttgc caagaacctt taacttcatt taatgctttg taattttcta
    17521 ttaataagtt tacttttcca tttataaacg attgttccat attgccctcc tagtaatata
    17581 tctcattgta ctaatagttt tatatttatt atatatatat taaatactac aatcaataca
    17641 tattacttca aagaacatca ttcttcaaca ataatgtagc cttttttgta atgtcttccc
    17701 atattattga ctctatcata gtactctgat tcactttcat tactccattt gggcacaaat
    17761 acatctattg cattagaatt actaaaatac ttactagacc ccctatccat aacagttttt
    17821 aattttccat ctacaataat cttagttcca aaacttaagt gattgtttgc tacaaaacca
    17881 tcttctaaat catctcctgt agcagttatc gctccatatc cattttcttg attaagactt
    17941 gtgtaataac taacttcaaa agttataggt actaacttct ttattttaat acctaactga
    18001 ttttgtagtt tctccttttg tactttcaac ttatactcat cactattttt ataatcttcc
    18061 ataccttgat tatacttgga attaaagctt ttacctgact ttactatagt atctatattt
    18121 actatagatt ctttacaatg atctgcatct atttttgttt ctccatttac caataatgga
    18181 gttaaacata cactacatac aacacatgta atcaatgctt ctttaagctt cataaattcc
    18241 ccccttatta ttttgatata ttagcatatt taatattata tgtctacatt tagatttaaa
    18301 taccacaccc atttgcttta agaagttgtc ttatatttaa ttcaccttaa acacttataa
    18361 atcctctatc ttagaatcat atagaataaa gcttttatct ttaaatattt tttattaatc
    18421 ttttaaatct aaaactactt tatctaattt tctctttttt agaatttaag aattaaaatt
    18481 ctataatttt aaaacatatt gaatttattt ttttgagatg atataatttt cttaagaggt
    18541 ggttgatat
//
LOCUS       DAGSWI010000079        18451 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.079, whole genome shotgun sequence.
ACCESSION   DAGSWI010000079 DAGSWI010000000
VERSION     DAGSWI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.079"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            447..1097
                     /locus_tag="MJF80_002448"
     CDS             447..1097
                     /locus_tag="MJF80_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBZ0281104.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLAQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1311..1982
                     /locus_tag="MJF80_002449"
     CDS             1311..1982
                     /locus_tag="MJF80_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281105.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKA"
     gene            1979..3109
                     /locus_tag="MJF80_002450"
     CDS             1979..3109
                     /locus_tag="MJF80_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0281106.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3541..3651
                     /locus_tag="MJF80_002451"
                     /pseudo
     CDS             3541..3651
                     /locus_tag="MJF80_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3977..6064
                     /locus_tag="MJF80_002452"
     CDS             3977..6064
                     /locus_tag="MJF80_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBZ0281107.1"
                     /translation="MQLTKIILSNYRCFGKEPTTIEINNLTGFIGHNSSGKTALLSAL
                     IKLFGEKSSDRIIERSDFHIPESTDPDSVDMNELYIETQFEFPELERDEDNLSIPVFF
                     KNMVVNEPGGKPYLRIRLEASWEKSSNPEGSIDTNIYYITSTTEDINPEDKRNIKRHD
                     LDQIKIIYVPAIRKPDEQLRNVSGTIISRLLNGISWSEQTKTKIKTKANETEKIFFEE
                     KGVESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTETHRDYRVE
                     ELGDGLRSLFYLSLVNTFLEIEEEILKSINDDKEEEIFTLIPPVLTIVAVEEPENHIS
                     PHLLGRVVEKLNLISKKENSQTILTSHSPAIVKRIEPTDIRYFRICKEHECTKVKSII
                     LPNKEKEVEQFKFVKEAVLAYPELYFAKLVVLGEGDSEEIIIKKMIELKAESVDSSEI
                     SIVPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLIKIGRDKNV
                     LLDTKDGILQDNEFKDMHTWNVTKTEIMLEWIKMLKEYDVYFSSPLDIDFAMLQSFKD
                     KYIATLESSQGPIIKGYGKIQNLEINEDTEEGLKNAYQARIVSDIKSTLKQEGGDGST
                     YTEEEKKLMIWYSYFFLGRGKPTTHLSLFSNNTDLDFESFPECLKEFVNRVIEKMIDD
                     LGDEEDNEEELDC"
     gene            6042..7913
                     /locus_tag="MJF80_002453"
     CDS             6042..7913
                     /locus_tag="MJF80_002453"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024642.1,HMM:NF024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBZ0281108.1"
                     /translation="MKRNWIVKEEWHPASGFKLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCKYPKKILAISFKKDAADNLKERVELRCGKELALRFESKTYDA
                     FAKEIVDRFRNSLDEKYRPLNNYNIATNGDVRRAFEISGLNFRGNQTEFNKLYGNKLS
                     INKLPLHTNFVDKIFIDAWNVLLKGKEDNNFESSLTFEMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTTSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNRFNEDAIDLKTNEKWNHEDGEAYLRLFE
                     DYIKEADILSEEIVGLVESGIKINEICILVKQSVDIYSKEIIHKLSECNIKARNEAVY
                     QELLKEDIIKILVSLFRLSLNDRSPDDWDYIYDILGELYGIDEGYNPKVLNDFITKVE
                     LMINDIKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQQYKSKDYFDDLVDKFKKLV
                     WSEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQTHDCINEFYELFDDTKIVKRIEYCD"
     gene            8033..11869
                     /locus_tag="MJF80_002454"
     CDS             8033..11869
                     /locus_tag="MJF80_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBZ0281109.1"
                     /translation="MKKKLKASKRILSFIMVFITIITTILTSSVSVYASELDMSEHTG
                     FNYLGISPITGRKINHNIYRMKMDNKVVFCVEPGIKTASGGGYISEEYINSKKDILSK
                     IAYYGYTDTNKSKYDYALTQIIIWEELGDKFISTTLPNYYKRKAEILEKVNKHNILPS
                     WNNEKVAVKSGETIELKDKNGIIREMKLESNNTGTNIVLEENTLKITANEKSTSGVIS
                     YRKIPENEVGTSIVYRKPNYQSLAEFHMASSMSAILNINVIKLGNIKAKKVDEDTGKA
                     LANTKLKFEYDGKGKEIITDSNGIANIEDIPEGTTVTISEVIAPNGYFNKGEIKKVVI
                     EANKTIEVTLGNKEQLGKVIISKSGKDFGIEMPNDSYKLEGAIYGIYNENDQKITTIT
                     TDKFGKATSENIKLGKYYALEEKAPEGYLISKEKIPFELKYAGQTVEVTSTSISQKDT
                     EQKGKAILIKEDEKTGSIPQGRAKLDGAVYELRKSNNDELVETVTIKGGKAKVENLYL
                     GKYYWIEKKAPEGYLLDTTKHYFEISYAGENVETAIKETLVKEKVITGGFDLIKFGEY
                     DWKEKIKNIFKTKNKEIKSLKDVEFSVFSDTTGKLVKTGYTDEKGYLKFERLPYDTYT
                     VKETKTPEGYIAAKDFKVTVKKQNETHHYAIQNRVIEEKLKVVKIDSVTKKIIPVKGA
                     EFKIKSLQTGDFITMPKNNEDEKTDMFYTNDEGYLITPEALSYGEYELTEVKAPDGYV
                     LAKDPIHFKVDGSSNGIIEIRFENTSQKGIVEFTKKGQTPKDIKIEEMKYGKLHEIIY
                     EYKPLENVAYDIIAKENITTNDGTIHFKKGDVVETLTTDEKGKWKSSELYLGKYQAIE
                     KSAPNGYIISSNPIEFELSYSGQLVELTKTSLTANNDLQSLLVNIFKNEEKIEGWKDN
                     KPVIKDIKGNGKIFGIFTREKQVISDNIKIPKDSMVGIKEVKDGVAELNIKLPEGKYY
                     LKEIDSGENHILNEKEYDFEFNSTNNEKEIKVNIYEDGVDFGDKNENIKPILNKLQLN
                     SFKIKKINEKAIKNKRNGFSFEFTELGQGSKFILEDKKGDIIQELTIGKGSIGEFNNI
                     PVGTFYLKEKETSNDKYILSNKVIRIESTKEGIKAFDEDNNLISEKNNNEDKILLEIK
                     NELIKGGAKLIKTDLVTGEALPNTGIRILNENKNTLIEGKTDDKGVFYFDNLPKGIYY
                     FQEYEAPKGYQIDETPMKFEIKENGEVVTCKMTNKKIEKVSIPKTGDYNSIILYGIVL
                     LASGTLLLKMRRKKDK"
     gene            12027..12341
                     /locus_tag="MJF80_002455"
     CDS             12027..12341
                     /locus_tag="MJF80_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBZ0281110.1"
                     /translation="MKLKHIIPNMEKTFGNLEYAGEGDVEQKRINGKMTVISRSYNLY
                     SNIQRADDIVVVLPANAGEKCFEVEERVKLINPKIIAEGYKIGTRGFTNYILLADDIV
                     KA"
     gene            12355..12696
                     /locus_tag="MJF80_002456"
     CDS             12355..12696
                     /locus_tag="MJF80_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBZ0281111.1"
                     /translation="MRLANGIVIDKEKTFGMLKFSALRREVRVQNDDGTPSNEIKERT
                     YDLKSRGQGRMIQVSIPASIPLKEFDYNAEVDIINPIADTVATANFNGTDVDWYIKAD
                     DIVLKNKKTSK"
     gene            12815..13834
                     /locus_tag="MJF80_002457"
     CDS             12815..13834
                     /locus_tag="MJF80_002457"
                     /inference="COORDINATES: protein motif:HMM:NF025637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281112.1"
                     /translation="MNQFKYEELESRYIYYMKNYNPIFNYVHDMGETQVIIDPIDKVC
                     RFCGKKYPEVKFKKKAHAISEMLGNKEFVLRNECDSCNIFFGQKLEDNLGKYLGLGRT
                     LSQIYGKEGVPSYKSRDRKSRVDFTDKGIVIQATIDSNFIEQYDNYIVFHSIRDTYTP
                     IAVYKALVKMALSLIPFEQMSLFKDTVEWLKEENHVISKHNMDNYTYVIERYVPGPKP
                     MQLRAMGFVRKYDVLLMPYYQFILEFANYSYQIIVPCKKKDKELCGHEIDFIPIPGTH
                     EVMCTATDRVLTELKYMGNKEKVKDEPLDICMNFEKCELYEGNGKSINEILKEEGIDL
                     SKRLK"
     gene            13886..15064
                     /locus_tag="MJF80_002458"
     CDS             13886..15064
                     /locus_tag="MJF80_002458"
                     /inference="COORDINATES: protein motif:HMM:NF027613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4935 domain-containing protein"
                     /protein_id="HBZ0281113.1"
                     /translation="MLKYPLSVVLDTNIFIGCRYDLNEEGLLIKLKNLVNNNKVRIYI
                     SNIVLRETERHIKADISKAIQELKQARRNISKNISPTIVNNTSLENIFGLPCQNTIEK
                     TAITRFREFLDESKVIYLDNEGIDIDEILEDYFKGNAPFENNEAKKYEFPDAFIISKL
                     KKEFHKNRPVWVVSADRGFRKALDSRDGFNCLASINELLDMINKQDQKMYDTIVEYIE
                     NKDAYKEICNKIEEKIESDDIEVNGLDYDRKGCCGGYEYIDTYITDVSVEKFYLSSVD
                     EISNDIVNLTIFCKAKIDVSCFYNDYDNSIWDSEEKEYMFLLNEEVDEEHEPEFECRL
                     SLKVNHYGDKVEFSLSDISYDLVLDQSSRTKQSLVEPEDPRIAAEADMMDALEEYYNH
                     "
     gene            15158..16561
                     /locus_tag="MJF80_002459"
     CDS             15158..16561
                     /locus_tag="MJF80_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBZ0281114.1"
                     /translation="MKFYRCRGKRIRASDKSLFFRFLTSILSAIFIISLILFNGKQIV
                     QINWKNISLVLENGLLFLSIFCNILTIVLSISICIVTTFLYYYFKRDRITQLIHRQKL
                     ARMILENKWYESESIKDDGFFKDLNSNKQKEKIVYFPKMYYELKEGLIYVKVEITLGK
                     YQDKLLNLEKKLESGLYCELVSKELKDSYIEYVLLYDTIANRISIDEIKVRNGSLKLM
                     KSVYWEFDKLPHMLIAGGTGGGKTYFILTIIEALLSTDSILYVLDPKNADLADLRTVM
                     PNVYYKKDDMISCINNFYDEMMKRSETMKAMSNYKTGENYAYLGLPANFLIFDEYVAF
                     MEMLGTKENTSVLNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGR
                     MSELGYNMMFGESNKEFFLKQIKGRGYVDVGTNVISEFYTPLVPKGHDFLKEIDKIIK
                     SRQVEQAACEAKADGSN"
     gene            16753..17940
                     /locus_tag="MJF80_002460"
     CDS             16753..17940
                     /locus_tag="MJF80_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBZ0281115.1"
                     /translation="MSIEAWIKNLRNKRIEYGISQNKLALSVGITREYLNKIESGKII
                     IKNDMKEKLIKSIERFNPDLPLTMMIDYIRIRFPTTDVKNVVNDILKLKLEYMIHEDY
                     GFYSYSEHYVIGNIFVLVSQDIEKGVLIELKGQGCRQFENFLLAQHRSWYDFLLEALL
                     AGGVMKRMDLAINDMTGILDIKELTNKCNNEECISVFRSFKSYRSGELVRRDEKIGMG
                     NTLYIGSLKSEVYFCIYEKDYEQYIKLDIPIDEAKIKNRFEIRLKNERAYHAAVDLLA
                     NRDAERTAFSIINRYIRFVDKQKDKSRSEWKINEHWNYFIGEGREKLKLTTSPEPYNI
                     HKTLNWLSRQVAPTLKTIKRLDILNNTTLIEDLIKYANLTDRHKKIIEQNSVNIEDII
                     YDE"
     gene            18009..18230
                     /locus_tag="MJF80_002461"
     CDS             18009..18230
                     /locus_tag="MJF80_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281116.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLMAIVVIGVYLGFKREFSKLIGFLV
                     VALIAVGLVFNAAGVKDILLKLFNQIIGV"
     gene            18295..>18451
                     /locus_tag="MJF80_002462"
     CDS             18295..>18451
                     /locus_tag="MJF80_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281117.1"
                     /translation="MRESVKNFLVKKYNELDYKSSCTCLKYHYKYNGVNVNIYFDAYD
                     LNSLSLYL"
BASE COUNT         7602 a         1748 c         3283 g         5818 t
ORIGIN      
        1 aatgtaaata tgacgaataa aaatgataaa tataataata tatatattta cattaggagt
       61 tttctatact ataattattt aaaactaaat ataaatgttg gaattattta ggacttaaaa
      121 ttgtctaaaa ataaatataa gtgatgaaga ggtgatgtga tgagattaga tatggatagt
      181 atgttatatg tactaattta tatgacatta ttggtatcta tcattggaag tatattttat
      241 ttttgtaaag acctcttaaa tcataaaatt aagtatgttt taataagaga catatttgta
      301 aatagaatta catattctga agaacggttg cctaaacagt atatagtttt tcagaaatat
      361 gatatttgga ggtattgtag tttgttttaa tttgaaatga atctattata aattctaaag
      421 tttaaaaata aaaaggagag aataaaatgg atttcatttc aagtatattg caaggattat
      481 taagtatatt atttaatttt acaggtgatt ttggaatttc tattgtactt ataactttaa
      541 ttgttaaatt agtacttatg ccactatcat taaaacaaag attttctgta aaaaaacagc
      601 aagaattagc tgaaaaaatg gaacatataa aagaaaagta taagaatgat actaaagaat
      661 tggaaaagca aatacaagta agttctgtag aaagtatgaa gagtatgctg ggatgtctta
      721 tagttttggc tcaaatgcca gtagtatatg ctttatatca tgtatgttta gacatgacaa
      781 aggaatttac aacaattata attccgtgga ttgctaattt aggtacattt gataatttgt
      841 ttatattacc ttgtatatat acattaatta tgttagcttc aaatttagta aattatatac
      901 catatttaaa aataaattct caagtaaagt ttaacaaaca aatggctgtg gtgactacca
      961 taacaagtct tatactaact gttagaactc cagtagccat aggtctgtac tttataacaa
     1021 gtgcaattta ttcattaatt gaagatattt gttttagaat atattttaat cgaaaaaata
     1081 gaatagtgac aaaataatat aattattatt agaaatattt tagcctgcta acatagaaaa
     1141 agttagtagg ctatttttat ctatgatatc aattccatta agaagagtct aaaaaatact
     1201 atcaaaatgc tataaaaaat actatcaaaa tactataaaa aaatagttga aaaataaata
     1261 aattaatata aactaaaata aagcctacaa aaaagaggtg ataaatagaa atggctaaaa
     1321 tactaattgt agaagatgaa aaaaggatgc aaagtattat tgtagaatat atgcaaaagg
     1381 gaggatatac ttgtataact gctgatgatg gagtcgaggc acttacaata ttaaagagca
     1441 acaatataga tttaatgata ttggatatta tgatgccgta tttagatgga ttttcagtgt
     1501 gtagagtttc acgtgaaatg actaatatac ctattattat attgacagct aaaggtgaag
     1561 aggaggacaa attaaagggt tatgaatatg gcgcagatga ttatataaca aaaccattta
     1621 gcccaaaagt acttttagca aaagttaatg ctttgttacg tagatacact actgatattc
     1681 ccaaaaattc attgagttta ggaaagattt ttattatggt agcatcaaga caagtgtatg
     1741 tagaagataa attgattgat ttaacatata aagaatttga attattgcgt ttatttatgg
     1801 aaaaccctaa tcaagttttt tccagagaga aacttttaaa ttgtatatgg ggatatgatt
     1861 ttgaaggaaa cacacgaact gtagatacac atataaaaac cttacgcaaa aagcttggta
     1921 gtgaaggaca ccatatagtt acattaattc gttcaggata taagtttgag gttaaggcat
     1981 gaatagttta agaaagatta tagaaaaact gaaaaatgta gatatttcaa attggattaa
     2041 acaaaatttt ggactatatt cagttagaaa aaaagtgttt ttactttcta aattatcagg
     2101 agcgttaatt atattatttt atctggttgt tgaagaattt tcgataaact ctaaatttga
     2161 attttggatt tggtttataa tactggttat atttattatg agtattgatt tcttattggg
     2221 aaaatttatt tcagaaccaa tcacatctat aaataaatct gcaaaaagta tgtcccaact
     2281 tgatttttct aatccatgta ctgtaaatac taatgatgaa tttggagaac tttctaggag
     2341 cctgaataca atgtctacaa atctacaaca ggcattatca gacctagaaa gtgcaaatat
     2401 tcaacttgaa aaggatgtaa ataaggaaag aatgctattg gaacaaagaa aggagttggt
     2461 agatacaatt tcacatgaaa tgaagactcc acttggcatt atacgtgcgt atacagaggg
     2521 gcttatggat gaagttgatg aagaaaaaaa gaaaaattat atgaatgtta taatagaaga
     2581 aactgataga atgaacaata tgattgtatc attattagac ttatctgcat tggaagcagg
     2641 agtttctaaa cttaacccag agcgttttga ttttatagag tttgttgaaa ctgtggcagg
     2701 tcgtctttta attgatatac cagatgccaa tttttatttt acatatgatt tgccagagta
     2761 tgaagttttt gtagtggcag ataaaatgag aatggaacaa gtagtagaga atctagttat
     2821 taatgcaaag aagcatgttg tatataatgg taatttagat ttatcgatta catgtgaaaa
     2881 tggcttgctt ttatttgaaa tttataatga tggtaggttt atagagcaag atgagatttc
     2941 aaagatttgg tctaagtttt atcgtagtgt tcaatctcag cgtacaggag gttcaggtct
     3001 agggcttgca attgtttcac aaatacttac aatgcaaggg ttagaatatg gagtgaataa
     3061 tcgtgataga ggagtagagt tttattttat gattccaatt gatgaataat ttagtattaa
     3121 tttataaatc ataagtatct gatgtaaaat atttttattt gtatatgtaa gcatttaaag
     3181 agaataaaat aaaaaaactt aatgaaaaaa gcttaatgaa aaagtctaat aaaaagttta
     3241 ataaaaagct taataaaaag tcttgttttc ctgatgaaaa caagattttt tattaagctt
     3301 tttattattt cacttcgatt tcacaagaga gacactctga tttcaccaaa tagaaataaa
     3361 ataggttaaa taaattaaaa aatagaagcg taattaaata aggttacaag ggggagtact
     3421 tatgaaatgg tatacatggt tgactattat attgataata ttgctttcta ttctgcttaa
     3481 aattaaattt ataaaatggt gggatagacg acaaaaaaat gataaaggag gttatgatag
     3541 atgataaaag taagagagtt aaaattttct tatggaaaag ataaacagat attgcatgga
     3601 cttaattttg aagtaaaaga aggagaaatc tttggattcc ttggacctaa tgaaccatag
     3661 atggatagat aataaaaaat aaaatctata atcacattaa aaagtcattt gtatatacag
     3721 atgacttttt tagtgaaata aaagaattta tgtatagtat atattcaata cttatatata
     3781 atatatcttc taaagaaaga ataatctttt atgaagaaaa agtagagtat ttaaaaagac
     3841 ttttgaaata ttgaacaata gaattatttt tattaatata atgatggaaa aaatttaggg
     3901 aaagatgtat aataaaggaa aatttcagaa ttaaaaacat aagatatgga attgggtttg
     3961 ggggaggaaa accattatgc aattaacaaa aataattctt agtaactata ggtgttttgg
     4021 aaaagaaccg acaactattg aaatcaataa tttaactgga ttcataggtc ataatagtag
     4081 tggtaaaact gctttattaa gtgcacttat aaaacttttc ggagaaaaat ctagtgatag
     4141 aattattgaa agatcagatt tccatatacc tgaaagcact gatccggata gtgttgatat
     4201 gaatgaatta tatattgaaa cacaatttga atttcctgaa ttggagaggg atgaagataa
     4261 cttatcgata ccagtatttt ttaaaaacat ggttgttaac gaaccgggag gaaaacctta
     4321 tttaaggatt aggcttgagg caagttggga aaaaagttca aatcctgaag ggagtattga
     4381 cacgaatata tactatataa cgtctacaac agaggatata aatcctgaag ataagcgtaa
     4441 tattaaaaga catgatcttg atcagataaa gattatttat gtacctgcca ttagaaagcc
     4501 agatgaacaa ttaagaaatg tatcagggac aataatttca agattactga atggtataag
     4561 ttggtcagaa caaactaaaa caaaaattaa aacgaaagca aatgaaactg agaaaatttt
     4621 ctttgaagaa aaaggggtcg aatctttaaa gaaatcatta gaaactcaat ggaatagtta
     4681 tcataaagat attagatact caaatccaaa gataaacttt aatagtactg atttagaaac
     4741 aattttaaag aaagtggaag taactttttc acctacagag acacatcggg actatcgtgt
     4801 tgaagaactt ggggatggtt tacgttcttt attttatctt tcgcttgtaa atacattttt
     4861 agaaattgaa gaagaaattc taaaatctat taatgatgac aaagaagaag agatatttac
     4921 attaatacca cctgtgttaa cgattgttgc agttgaagag ccagaaaatc atatttcacc
     4981 tcatctttta ggaagagtag ttgaaaaatt gaatcttatt tctaaaaaag aaaattcaca
     5041 aacaatattg acatcacatt caccagctat agtgaaaaga attgaaccaa cagatataag
     5101 atattttaga atttgtaagg aacatgaatg tacaaaagtg aagtcaatta tattaccaaa
     5161 taaagaaaag gaagtagaac aatttaaatt tgtaaaggaa gcagtattag catatcctga
     5221 actttatttt gcaaaacttg ttgtattggg tgaaggtgac agtgaagaga ttattattaa
     5281 gaagatgatt gaattaaaag cagaatctgt agattcatca gaaatttcaa tagtaccatt
     5341 aggtggtagg catgttaatc atttttggcg tttattaaat gatttagaaa ttccatttat
     5401 tacactatta gatttagata gagaacgaga tggaggtggt tggggacgta taaagtatgc
     5461 aatacaacaa ttgataaaga ttggtcgtga taagaatgta ttacttgata caaaggatgg
     5521 aattttacaa gataatgaat ttaaagatat gcatacttgg aatgtaacta agactgaaat
     5581 tatgttggaa tggattaaaa tgcttaaaga gtatgatgtt tatttttcat ctccgttaga
     5641 tatagatttt gcaatgttac aaagttttaa ggataaatat attgcaacat tagaaagtag
     5701 tcaagggcct ataataaaag ggtatgggaa aatacaaaat cttgaaatta atgaagatac
     5761 tgaagaggga ctgaaaaatg cttatcaagc tagaatagtt agcgatatta aatcaacatt
     5821 aaaacaagaa ggtggcgatg gatctactta tactgaagaa gagaaaaaac ttatgatctg
     5881 gtattcatat ttctttttag gaagaggtaa acccactact catttatctt tattttcaaa
     5941 taatactgat ttagattttg aaagttttcc agaatgtcta aaggagtttg taaatagagt
     6001 tattgaaaag atgatagatg atttagggga tgaagaagat aatgaagagg aattggattg
     6061 ttaaggaaga gtggcatcca gcatcaggat ttaaattaga agttgaggcg atgaatacag
     6121 ttattaatga taaaaataca cttgttgtag ctggtcctgg agcgggaaaa acagaattat
     6181 tagcacagag agcttgcttt ttgttagaga ctaatacatg taaatatcct aaaaaaatcc
     6241 ttgctattag ttttaaaaag gatgctgctg ataatttaaa agagcgtgta gaattaagat
     6301 gtggcaaaga attagcttta agatttgaat caaagacata tgatgctttt gcaaaagaaa
     6361 tcgtagatag atttagaaat agtttagatg aaaaatatag acctttaaat aattacaata
     6421 tagctacaaa tggtgatgtt agaagagcat ttgagatatc aggattaaat ttcagaggaa
     6481 atcaaactga atttaataaa ttatatggaa ataaattatc gataaataaa ttgcctttac
     6541 atacaaattt tgtagataag atttttatag atgcttggaa tgtactactt aaaggtaaag
     6601 aagataataa ttttgaaagt tcacttactt ttgaaatgat atcacgttta gcagaatatt
     6661 tgataagaac caataggtat attaaaaaag caattgaaat gacttatagt catgtgtttt
     6721 tagatgagtt tcaagatact acatctattc aatatgatct tgttaaagcc tgttttttta
     6781 aaacagaaac aataattact gctgttggtg atggtaaaca acgaattatg ttatgggctg
     6841 gtgctagaaa atcaatattt gaagattttc aaaatgattt tttaagtcaa aaaagaacat
     6901 taattatgaa ccatcgttca gcgccaagac ttgtagagat tcaaaaagca atgtataaca
     6961 gattcaatga agatgcaatt gatttaaaaa ctaatgaaaa gtggaatcat gaagatggag
     7021 aagcttattt aagacttttt gaggattata taaaagaagc tgatatactt agtgaagaaa
     7081 tagtcggatt ggtagaaagc ggaataaaaa ttaatgaaat atgtatatta gtaaagcaga
     7141 gcgtagatat ttattcaaaa gagattatac ataaactttc tgaatgtaat attaaggcac
     7201 gtaatgaagc agtatatcag gaactattaa aagaagatat aataaagata cttgtttcgc
     7261 tttttaggtt gtcattaaat gacagaagcc ctgatgattg ggattatatt tacgatattc
     7321 taggtgaatt atatggaata gatgaaggtt ataatccaaa agtattaaat gattttatta
     7381 ccaaagtgga attaatgatt aatgatatca aagtaaattt aaatattact gatgaaaaac
     7441 agttctcaga tttagttgat tctaaggttg agtatatttc atacgaaaaa tttgcatcaa
     7501 agtttcaaca atataaatct aaagattatt ttgatgattt ggtagataaa tttaaaaagc
     7561 tagtatggag tgaatataat gaaactaagg attggattaa agcgattgat aactttgaag
     7621 ggaaaaattc aattccaata atgacaattc ataaaagcaa aggacttgaa tatgatacta
     7681 tcttttttgt tggtttagaa gactcagcat tttggaattt taaaaatcaa ccagatgaag
     7741 atagatgtgc attttttgtt gcattatcaa gagctaaaag aagaattgat tttacattta
     7801 gtttagatag accagcagga ttttcgaatt cgcagacgca tgattgtatt aatgaattct
     7861 acgaattatt tgatgatact aagattgtaa aaaggataga gtattgtgat taagattatg
     7921 aaaaatttag tatattatat tttaagatat tagttatatg agtagacatt gaatttatat
     7981 attcgatgtc tattttttat ataaaaatat attgaagaaa ggatgatagt taatgaagaa
     8041 aaagttaaaa gcatctaaaa gaattttaag ttttattatg gtttttataa ctataattac
     8101 tactatactt acatcatcgg tttcagttta tgctagtgag ttggatatga gtgaacatac
     8161 aggatttaat tatttaggta ttagtccaat tacaggaaga aaaattaatc ataatattta
     8221 tcgtatgaaa atggataata aagttgtatt ttgtgtagaa ccaggaatta aaacagcaag
     8281 tggaggcgga tatatatcgg aggagtatat aaattcaaag aaagatatat tatctaagat
     8341 agcttactat ggatatacag atacaaataa atcaaaatat gactatgcat taactcaaat
     8401 aattatatgg gaggagttag gagataaatt tatatctaca acacttccca attattataa
     8461 aagaaaagct gaaatacttg aaaaggtaaa taagcataat atattacctt catggaacaa
     8521 tgaaaaagtt gcagttaaaa gtggagaaac aattgagtta aaagataaga atggcattat
     8581 tagagaaatg aaactagaat caaataatac gggaacaaat atagtacttg aagaaaatac
     8641 tttaaaaatt acagcaaatg aaaaatcgac ttctggagtt attagctata gaaaaattcc
     8701 agaaaatgag gttggaacat caatagtcta tcgtaagcct aactatcaaa gcttagcaga
     8761 gtttcatatg gcaagttcaa tgagtgcaat tttaaatata aatgtaataa agttaggaaa
     8821 cattaaagca aaaaaggttg atgaagatac aggaaaggca ttagcaaata ctaagcttaa
     8881 atttgagtat gatggtaagg gcaaagaaat tataacggat tctaatggaa ttgcgaatat
     8941 agaagatata ccggaaggaa caactgtaac aatatcagaa gtgatagcac caaatggata
     9001 ttttaataaa ggtgaaatta aaaaagttgt tattgaagca aataaaacta tagaagttac
     9061 attaggaaat aaagagcagt taggtaaagt aataattagt aagagtggaa aagattttgg
     9121 aatagaaatg cctaatgata gttataaatt agaaggagca atttatggaa tttataatga
     9181 aaatgatcaa aagataacta ctataaccac agataaattc gggaaggcaa catcagaaaa
     9241 tataaagctt ggaaagtatt atgcacttga agaaaaggct ccagagggat atttaataag
     9301 taaagaaaaa ataccatttg aattaaaata tgctggacaa actgtagaag taacatcaac
     9361 atctattagt caaaaagata cagaacaaaa aggaaaagca atattaatta aagaagatga
     9421 aaaaactgga tcaatacctc aaggacgtgc aaaacttgat ggtgcagttt atgaattaag
     9481 aaaatcaaat aatgatgagt tagttgaaac agtaacgatt aaagggggaa aagcaaaagt
     9541 agaaaattta tatcttggta agtattattg gattgaaaag aaagctccag aaggatattt
     9601 attagatact actaaacatt attttgaaat aagttacgca ggtgaaaatg tagaaacagc
     9661 tataaaagaa acactagtaa aagagaaagt aattacaggt ggatttgatt taataaagtt
     9721 tggagaatat gattggaaag aaaaaataaa aaatattttc aaaacaaaaa ataaagaaat
     9781 aaaatcttta aaagatgtag agttttctgt atttagtgat acaacaggaa agttagttaa
     9841 aacaggatat acagatgaaa aagggtatct aaaatttgaa agattaccat atgatactta
     9901 cacagtaaaa gaaactaaaa caccagaagg atatattgca gcaaaagatt ttaaagtaac
     9961 agttaaaaaa caaaatgaaa cacatcatta tgcaatacag aatagagtaa ttgaagaaaa
    10021 attgaaagtt gtaaagatag attcagtaac taagaagata ataccagtaa agggagctga
    10081 atttaaaata aaaagcttac aaactggaga ttttataaca atgcctaaaa ataatgaaga
    10141 tgaaaaaact gatatgttct atacaaatga tgaaggatat ttaataacac cagaagcatt
    10201 gtcatacgga gaatatgaat taacagaagt taaagctccg gatggttatg tattagcaaa
    10261 agatcctata cattttaaag tagatggttc atcaaatggg attattgaaa taagatttga
    10321 gaatacatca caaaaaggta ttgttgaatt tacaaagaaa ggacaaacac ctaaagatat
    10381 aaaaatagaa gaaatgaaat atgggaaatt acatgagata atatatgaat ataaaccttt
    10441 agaaaatgtg gcctatgaca ttatagcaaa agaaaatatt actacaaatg atggaactat
    10501 acacttcaag aaaggcgatg ttgtagaaac acttacaact gatgaaaaag gaaaatggaa
    10561 atcatcagaa ttgtatctag gaaaatatca agctattgaa aagagtgctc caaatggata
    10621 cataatatcc tcaaatccga tagaatttga attaagttat tcaggtcagc tagttgaatt
    10681 aactaaaact agtcttacag caaataatga tttgcaatct ttattagtta atatatttaa
    10741 aaatgaagaa aaaattgaag gatggaaaga taacaaacct gtaataaaag atattaaagg
    10801 taatggtaaa atatttggaa tatttactag ggaaaaacaa gtgatatctg ataatattaa
    10861 gattccaaaa gattcaatgg taggaattaa agaggttaaa gatggagttg ctgaattaaa
    10921 tataaaacta ccagaaggta aatattattt aaaagaaata gattcaggag aaaatcatat
    10981 tttaaatgag aaagaatatg attttgaatt taactcaacc aataatgaga aggaaataaa
    11041 agttaatatt tatgaagatg gtgtagactt tggagataag aatgaaaata taaaaccaat
    11101 attaaataaa cttcaattaa atagctttaa aataaaaaag attaatgaaa aagctataaa
    11161 gaataaaaga aatggattta gctttgagtt tactgaatta ggtcaaggat ctaagtttat
    11221 attagaagat aaaaaaggag atataataca agaattaaca ataggaaaag gctcaatagg
    11281 cgaatttaac aatataccag taggaacatt ttatttaaaa gaaaaagaaa catctaatga
    11341 taagtatata ttatctaata aagtaatacg tattgaatca acaaaagaag ggataaaagc
    11401 ctttgatgaa gataataatt taatttcaga aaaaaataat aatgaagata agatattact
    11461 tgaaattaaa aatgaattga ttaaaggagg agctaagtta atcaagactg atttagtaac
    11521 aggggaagct ttacctaata caggaattag aatattaaat gaaaataaaa atactttaat
    11581 agaaggtaaa acagatgata aaggtgtatt ttattttgat aatttaccta agggaatata
    11641 ttacttccaa gagtatgaag cacctaaagg ttatcaaatt gatgaaacac caatgaaatt
    11701 tgaaataaag gaaaatggtg aagttgtaac ttgcaaaatg actaataaga aaatcgagaa
    11761 agtttctata cctaaaacag gagattacaa tagcattatt ttatatggaa tagtattgct
    11821 agcatcagga acattattat taaaaatgag acgtaaaaaa gataaataga agtatgtgaa
    11881 tgtcagatac aaataagtat ctgttttttt atacaatttt ttaggggagg taccatgaat
    11941 tttaaagtag tgtttattag atcaccgaca cttaaaagta ttaatttaaa gaattatata
    12001 attttagaaa agagaggtaa atgattatga aattaaaaca tattattcct aacatggaga
    12061 agacatttgg aaatttagaa tatgctggag aaggcgatgt tgaacaaaaa agaataaacg
    12121 gtaaaatgac agttatatct agatcatata atctttattc taatatacag agagcagatg
    12181 atatagttgt tgttttacca gctaatgcag gtgaaaaatg ctttgaagtt gaagaaaggg
    12241 taaagttaat aaaccctaaa attatagcag aaggatataa aataggaact cgtggtttta
    12301 caaattatat tttattagca gatgatatag taaaggctta aggaggatga gattatgaga
    12361 ttagcaaatg gaatagttat agataaagaa aaaacatttg gaatgttaaa gttttcagca
    12421 ttaagaagag aagtacgtgt tcaaaatgat gatgggacgc catcaaatga aattaaagaa
    12481 cgtacttatg atttaaagag taggggacaa ggaagaatga tacaagttag tattccagca
    12541 tcaatacctt taaaagaatt tgattataat gcagaagtag atatcattaa tccaattgca
    12601 gatacagtag ctacagctaa ttttaatggt actgatgtag attggtatat taaagctgat
    12661 gatatagtat taaaaaataa aaaaactagt aagtaaaaat caaaaataat acattaataa
    12721 gtttacttat atagtataaa gagatataat atttttacaa ataatggtct aatgtttata
    12781 ctatttagta ttagaaataa ggggatgggg aattgtgaat caatttaaat atgaagagct
    12841 agaaagccgt tatatatact atatgaaaaa ctataatcct atttttaatt atgttcatga
    12901 tatgggagaa actcaggtta ttattgatcc tattgataag gtttgtagat tttgtggtaa
    12961 aaaataccca gaagtgaaat tcaagaaaaa agcacatgct atatctgaaa tgcttggtaa
    13021 taaagaattt gtattacgaa acgagtgtga ttcttgtaat atcttttttg gtcagaagtt
    13081 agaagataac ttagggaaat acttaggtct tggtagaacc ttatcacaaa tatatgggaa
    13141 agaaggagtt ccatcttata aatcaagaga taggaaaagt agagtagatt ttacggataa
    13201 aggtatagtt attcaagcaa ctatagatag caattttata gagcaatatg ataattatat
    13261 agtatttcac tcaatacgcg atacctatac tccaattgct gtttataagg ctttagtaaa
    13321 aatggcatta tcacttattc catttgaaca aatgtcactg tttaaggata ctgttgagtg
    13381 gttaaaagag gagaatcatg ttatctctaa acataatatg gataattata cttatgtgat
    13441 agagagatat gttccaggtc caaagcctat gcaattaaga gctatgggat ttgttagaaa
    13501 atatgatgtt cttttaatgc cttattatca gtttatatta gaatttgcaa actatagtta
    13561 tcagattatt gtaccatgta aaaaaaagga taaagaatta tgtggacatg aaattgattt
    13621 tataccaata cctggaacac atgaagtgat gtgcacagct acagatagag ttttaacgga
    13681 attaaagtat atgggcaata aagaaaaagt aaaagatgaa cctttagata tttgcatgaa
    13741 ctttgaaaaa tgtgaattat acgaagggaa tggaaaaagt ataaatgaaa tattaaaaga
    13801 agaaggaatt gatttgagca agagattaaa ataaataatt aagtattcaa tctaatttaa
    13861 tgttcaatat gaaggaggga aataagtgct taagtatcca ttatcagtag ttttagatac
    13921 aaatatattt ataggatgta gatatgattt aaatgaagaa ggtttactta taaagcttaa
    13981 aaacttggtg aataataata aggttaggat ttatataagt aacattgttt taagagaaac
    14041 tgagagacat ataaaagcag atatttcgaa ggcaatacag gagttaaaac aagctagaag
    14101 gaacatatca aaaaatattt ctccgactat tgtaaataat acatcacttg agaatatatt
    14161 tggattgcca tgccaaaata caatagaaaa aactgctatt actagatttc gagaattttt
    14221 agatgagtct aaggtaatct atcttgataa tgaaggtata gatattgatg agattctaga
    14281 ggattatttt aaaggtaatg ctccatttga aaataatgaa gcaaaaaaat atgaatttcc
    14341 tgatgcattt attatatcaa agttaaaaaa agaatttcat aaaaatagac ctgtatgggt
    14401 agttagcgca gatagaggat tcagaaaggc attagacagt agagatggtt tcaactgctt
    14461 agcttcaatt aatgagcttt tggatatgat aaataaacaa gatcaaaaaa tgtatgatac
    14521 gattgtagaa tatattgaaa ataaagatgc atataaagaa atatgtaata aaatagaaga
    14581 aaaaatagaa tctgatgata ttgaagttaa tggactagat tatgatcgta aaggatgctg
    14641 tggaggttat gaatatatcg atacttatat tacggatgtt tccgttgaaa agttttatct
    14701 tagttctgta gatgaaatta gtaatgacat agttaacttg acaatattct gcaaagcaaa
    14761 aattgatgtg tcttgtttct acaatgacta tgataactcg atttgggatt ctgaggaaaa
    14821 agaatatatg tttttattaa atgaagaagt tgatgaagaa catgagccag aatttgaatg
    14881 tagattaagt ctgaaggtta atcattatgg tgataaagtt gaattttcat tatctgatat
    14941 ttcatatgat ttggttttgg atcaaagttc aaggacaaaa cagagtttag ttgaaccaga
    15001 agatcctaga atagcagcag aagcagatat gatggatgct ttggaggagt attacaacca
    15061 ttagcaagta ggataactat aatagatttt cattaatatt tgaaacagta gatttgtaaa
    15121 atttactgtt ttttattttt actaaaaagg agagaaaatg aaattttata gatgtagagg
    15181 aaagcgaata agagctagcg ataaatcact gttttttcgc tttttaacaa gtatattatc
    15241 agctatattt atcatatcat tgatactttt taatgggaaa cagatcgtac aaataaattg
    15301 gaagaatata agcttggttt tagagaatgg tttattattt ttatctatat tttgtaatat
    15361 attaacgata gtattatcta ttagtatttg tatagttact acttttttat attattactt
    15421 taaacgagat agaataacgc aacttattca taggcaaaaa ctagctagaa tgatattaga
    15481 aaacaaatgg tatgaatcag agtctattaa agatgacgga ttttttaaag acttaaattc
    15541 taataaacag aaagaaaaaa tagtatattt ccctaaaatg tattatgaat taaaggaggg
    15601 gttaatttat gttaaagttg aaataacttt aggcaagtat caggataagt tattaaattt
    15661 agagaagaag ttagaatcag gtttatattg tgagttggtt tctaaagagt taaaagattc
    15721 ttatattgag tatgttttac tgtatgatac tattgcaaat agaatatcca ttgatgaaat
    15781 taaagttcgt aatggaagtt taaaacttat gaagtctgta tattgggagt ttgataaatt
    15841 accacacatg ctaattgcag gaggaactgg agggggaaag acatatttta tacttacaat
    15901 tattgaagca ttactaagta cagactctat actatatgtt ttagatccta aaaatgcgga
    15961 tctcgctgat ttaagaacag ttatgccaaa tgtttattat aaaaaagatg atatgatttc
    16021 atgcataaac aatttttatg atgaaatgat gaagcgtagt gaaactatga aagctatgag
    16081 taattataaa actggagaaa actatgctta tcttggactg ccagctaact ttttaatatt
    16141 tgatgaatat gttgccttta tggaaatgtt aggaactaaa gaaaatactt ctgttttaaa
    16201 taaattaaag caaatagtaa tgttaggtag acagtctgga ttttttctta tattggcttg
    16261 ccaacgtcca gatgcgaagt atcttggtga tggtattcgt gatcaattca attttagagt
    16321 agcacttgga aggatgtctg agcttggata taatatgatg tttggggaat caaataaaga
    16381 gtttttctta aaacaaatta agggtagggg atatgtagat gtaggaacaa atgttataag
    16441 tgaattttat accccgttag taccaaaagg tcatgatttt ttgaaagaaa tagataaaat
    16501 tattaagagc aggcaggtag aacaagcggc gtgcgaagcg aaagccgatg gttcgaacta
    16561 gcctgctatt ggtatggctt tgccatacca atatttatta gcccccggta tctaacaggg
    16621 gggcacaaat cgactaaaat catttaagca aaacttataa acgtaaggta gaaacaatgt
    16681 tataagaaat taaaggtatg tgaaaaaatt taatgttatt tcacatacct tttttaatag
    16741 gaggataagc aattgagtat agaagcatgg attaaaaatt taagaaataa aaggattgaa
    16801 tatggtattt ctcaaaacaa actagcatta tcagtaggta ttacaagaga gtacttaaat
    16861 aaaatagaaa gtgggaaaat aataataaaa aatgatatga aagagaagtt aattaaatca
    16921 atagaaagat ttaatccaga tttgccacta acaatgatga ttgattatat aagaataaga
    16981 tttccaacaa cagatgtaaa aaatgttgtt aatgatatat tgaaattaaa acttgaatat
    17041 atgatacatg aagattatgg attttactct tactcagagc attacgttat aggcaatata
    17101 tttgttttag tatctcaaga tatagaaaaa ggagttttaa tagaattgaa aggacaaggg
    17161 tgtagacaat ttgaaaactt cttattagca caacatagat catggtatga ctttttatta
    17221 gaggcattat tagctggagg cgttatgaaa agaatggacc ttgctattaa tgatatgaca
    17281 ggaatactag acataaaaga attaacaaat aaatgtaata atgaagaatg tatttctgta
    17341 tttcgtagtt tcaaatcgta tagatcaggt gaattagtta gaagagatga aaaaataggt
    17401 atgggaaata ctttatatat tggttcatta aaaagtgagg tttatttttg tatttatgaa
    17461 aaagattatg agcagtatat aaaactggat atacctattg atgaagcaaa aataaaaaat
    17521 agatttgaaa taagattgaa aaatgaaaga gcatatcatg cagcagttga tttattagca
    17581 aatagagatg ctgaaagaac agcattttca attataaata gatatatacg ttttgtagat
    17641 aaacagaaag ataaaagtag gagtgaatgg aagattaatg aacattggaa ctattttata
    17701 ggtgaaggca gagaaaaatt aaaattaaca acaagtccag aaccgtacaa tattcataaa
    17761 actttaaact ggctatcgag acaagttgca ccaactttaa aaactataaa aagattagat
    17821 atattaaata acacaacatt aattgaagat ttaataaaat atgcaaactt aacagatcgt
    17881 cataaaaaaa taattgaaca aaattcggtg aacattgaag atattatata tgatgagtaa
    17941 aagaaagtgt agttggttca atagataaag caatatagat ttaataaaaa agaagggaga
    18001 tttgagaaat gaattttggt caaaatttat ataactggtt tttatcaaat gcacaatcat
    18061 tagtattaat ggctatagtt gtaattggtg tatatctagg attcaaaaga gagttttcaa
    18121 aacttatagg atttttagtt gttgcattaa ttgcagtagg tttagtgttt aatgcagctg
    18181 gagttaaaga tatattactt aagttattta accaaattat cggtgtttaa aattggaaaa
    18241 tattacatta actatgatat aatatgtcca taaaacgaat aaggaggaca ttatatgagg
    18301 gagagtgtta aaaattttct agttaaaaaa tataatgaat tggattacaa atcttcttgc
    18361 acttgtttaa aatatcacta taaatataat ggtgtaaatg taaatatata ttttgatgca
    18421 tatgatttaa atagtttatc tttatatctt g
//
LOCUS       DAGSWI010000080        18262 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.080, whole genome shotgun sequence.
ACCESSION   DAGSWI010000080 DAGSWI010000000
VERSION     DAGSWI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.080"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            344..1135
                     /locus_tag="MJF80_002463"
     CDS             344..1135
                     /locus_tag="MJF80_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBZ0281118.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALTAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            1164..2384
                     /locus_tag="MJF80_002464"
     CDS             1164..2384
                     /locus_tag="MJF80_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBZ0281119.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            2404..3099
                     /gene="pxpB"
                     /locus_tag="MJF80_002465"
     CDS             2404..3099
                     /gene="pxpB"
                     /locus_tag="MJF80_002465"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBZ0281120.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            3129..4115
                     /locus_tag="MJF80_002466"
     CDS             3129..4115
                     /locus_tag="MJF80_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBZ0281121.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(4334..4900)
                     /locus_tag="MJF80_002467"
     CDS             complement(4334..4900)
                     /locus_tag="MJF80_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281122.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            5252..6319
                     /locus_tag="MJF80_002468"
     CDS             5252..6319
                     /locus_tag="MJF80_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBZ0281123.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESVILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            6338..7498
                     /locus_tag="MJF80_002469"
     CDS             6338..7498
                     /locus_tag="MJF80_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBZ0281124.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAINMSLGL"
     gene            7511..9607
                     /locus_tag="MJF80_002470"
     CDS             7511..9607
                     /locus_tag="MJF80_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBZ0281125.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTVITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(9766..10110)
                     /locus_tag="MJF80_002471"
     CDS             complement(9766..10110)
                     /locus_tag="MJF80_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBZ0281126.1"
                     /translation="MEIKRYEGTGRMSRTVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            10402..11292
                     /locus_tag="MJF80_002472"
     CDS             10402..11292
                     /locus_tag="MJF80_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBZ0281127.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            11510..12796
                     /locus_tag="MJF80_002473"
     CDS             11510..12796
                     /locus_tag="MJF80_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBZ0281128.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(12885..13328)
                     /locus_tag="MJF80_002474"
     CDS             complement(12885..13328)
                     /locus_tag="MJF80_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBZ0281129.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(13352..14146)
                     /locus_tag="MJF80_002475"
     CDS             complement(13352..14146)
                     /locus_tag="MJF80_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBZ0281130.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(14712..15188)
                     /locus_tag="MJF80_002476"
     CDS             complement(14712..15188)
                     /locus_tag="MJF80_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBZ0281131.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDDPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(15289..16458)
                     /locus_tag="MJF80_002477"
     CDS             complement(15289..16458)
                     /locus_tag="MJF80_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0281132.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSTNIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            16621..17484
                     /locus_tag="MJF80_002478"
     CDS             16621..17484
                     /locus_tag="MJF80_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBZ0281133.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDTKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            17555..18148
                     /locus_tag="MJF80_002479"
     CDS             17555..18148
                     /locus_tag="MJF80_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBZ0281134.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
BASE COUNT         6992 a         2115 c         3117 g         6038 t
ORIGIN      
        1 tgaatgaaga aaaatgattc tgtcttgcat ataaagtgat taaaaatgaa aaatacttta
       61 aaaatgaagg ttaaagccct tattttaagt gaaaataatt aattattaac taaaaaatgg
      121 taaaaagatt ttttgaaaaa gaaatacaaa tgaatgttca attaaaataa ttgcaaaaat
      181 attccttaaa tggtaaaatt atattaatta aatgcaatag aatagttgca aaaataaaat
      241 tgaatacaag tgataaaata tttcatgcaa tatataaaat aaacaaaatt taaaaattaa
      301 aactacttat tctatcaaaa tgttagcgag gaggggtcat aaattgtata aagtagattt
      361 aaatagtgat ttaggagaaa gttttggaac gtataagatt ggtttagatg aagaagtttt
      421 aaaatatatt tcatcagcta atatagcttg tgggtttcat gcaggtgacc ctagtcatat
      481 ggaaaaaaca gtacagctag ctaaaaaaaa tggagtgaaa ataggcgcac atccaggttt
      541 cctagattta ataggatttg gaagacgtga gatgaaaata acaaagcagg aagctaaaga
      601 ttatacaaaa tatcaattgg gtgctttaac ggcatttgca agttcaaatg gatgtaatat
      661 acaacatgta aaaccacatg gagcattata caatatggca gctaaagata aagagttagc
      721 tatggggatt tgtgaagcta tatatgaagt cgataaagat attatactgt tggggctata
      781 taatagtgaa atgataaatt ctgctaaaga aattggactt aggtttgcaa atgaagtatt
      841 tgcagataga gcttatgata ataatggttt tttggttcca agaaatgtag aaggagcagt
      901 tatacatgat acaaaacatg caatagatag ggttgttagg atggtaaaag aaggaactgt
      961 agaaacttta acaggtgaag ttatacatat caaagcagat tctatatgtg tacatgggga
     1021 caatccaaaa gcaatagagt ttgtcaaaga aataagaaag cgttttgaat tagaaggtat
     1081 agaagtttgt ccattagaaa acatagaagt ttgttcgtca gagaacatag tataagactg
     1141 ataacatgag ggggagataa aatatgagta caaaaaatat tacagataaa actaaaaata
     1201 aaaaagatgt cggagcactt ataggggctg catttataat ggcaacatca gcaattggac
     1261 cagggttttt aactcagact gcacaattta cccaagattt tggaccaaat ttttcatttg
     1321 taattttaat tacaacaatc ctttttatag gggctcaagt aaatgtatgg agagtaattg
     1381 gtgtatcagg acttagaggt caggatatag caaataaaat aatacctgga ttaggttatt
     1441 tagtagcttt tttagtagct cttggaggat tagcttttaa tataggaaat gttggtggag
     1501 ctgcacttgg tatgaatgta atgtttaata tgaatatgac attaggaact gtattaagtg
     1561 gattaatagc tatatttgta tttatgtcaa aaaattcaaa ttctctagtc gataagataa
     1621 ctaaattttt agcattagga atgataatta tagttggata tgtagcaata tcaaatcatc
     1681 ctcctgttgg agaggcagta tcaagaatgg taaaaccaga aaatccaaaa ggactgatat
     1741 tcccaataat aactctactt ggaggaagtg ttggaggtta tataactttt gctggaggac
     1801 atagattaat agacggtgga ataacaggag aagaaaatat aaaagaaata actaaatctt
     1861 ctcttttggg tatattagtt gccacaatga tgagagttct tttattttta gcaatactgg
     1921 cagtggtttc aaaaggtctt caacttgacc cagaaaatcc agctgcttca gcttttaaat
     1981 ttagtgcagg agcaatagga tataaattct ttggacttgt attatggtct gctgcaatta
     2041 catcagttat aggagctgct tatacatcag tttctttctt aaagacacta aatcctttta
     2101 tagataaata tgaaaaatat tttataatag catttatagc aatatcaaca cttataatgg
     2161 catttatagg aaaaccagct actttattaa ttttagcagg agctttaaat ggattgatac
     2221 ttccaatcac attaggaatt atgcttatag catctaaaag aaaagatatt gttggagatt
     2281 acaagcatcc tacatggctt ttaatatttg gattaatagt agttttaata tctgcatata
     2341 caggaataac ttctcttagc agcctaggtg ctttatttgc ttaatttaga agaggtgaca
     2401 tttatggaaa ctagatactt gatatcaggg gataaggcag tggttgctga atttggaaat
     2461 gagatatcag aagatataaa caaaaaagta atttcattta tgagagcaat agaaatatcg
     2521 aatcttaaag gtattgtaac agaaatggta ccaacatata ggtcacttat gataagttat
     2581 aatccattag aaatagattt tgatagtttg attgagaatt taaaaaaaat agaagataat
     2641 ttggaaagta tagtattacc aaaacctaaa atacatgaga ttccagtatg ttatgatgag
     2701 gtttttggta tagatattaa aaacgttgca agctacaata atctcactgt agatgaagtt
     2761 ataaaaatac acacaagcag agagtacttg atatatatgc tagggtttac tcctggattt
     2821 ccatatctag gtggtatgga tgaaagaata gctacaccta gattagaagt gccaaggact
     2881 aaaatttatg gtgggagtgt tggaatagca ggttcccaga ctggtgtata tcctattgat
     2941 agtcctggtg gatggcaaat aattggtagg acacctttaa aattatatga tgaaaataga
     3001 gaagaacaga ttttgcttag agctggagat tttataaaat ttgttccaat aacattagat
     3061 gaatttatag aaatcgaaaa aaataactta agtatttaaa tatcgttata gtacagggag
     3121 tggtatatat gggattcaaa gttgaattag gaggtttcca aacattaata caagacagag
     3181 gtagagttgg ctatggtcaa tatggtgtat ctggatgtgg agcaatggat gagtatgcac
     3241 atagagtagg aaatatatta gtaggaaatt ctgaagatga ggcttcttta gaagttttaa
     3301 tgttaggtcc tacaattacc tttgatgaat atacgcaaat agcagtaact gggggagatt
     3361 taggggctaa aataaatgga aaagaaattc aaaactggag gtcatatcaa ataaatccag
     3421 gtgatgtatt atcatttaga ggtgtgaaat caggtgctag agcctatgtg tcaatagctg
     3481 gtggaataga tgttccactt gcaatgggaa gtaagtcaac ttatacaaga gctaaaattg
     3541 gtggatttga aggtagagca cttaaaaaag gcgattatat aaatactttt attcaggaaa
     3601 aagattttac aataaataaa aaattaagtt caaaatatat tccaacttat agttcagaaa
     3661 tcgtactaag aattgtaaaa ggtcctcaat ttgatgcttt ttctaacggt gaagtagaga
     3721 aatttttatc aaataaatac aaagttacaa atgaaataga tagaatgggt tgtagactag
     3781 atggagagag tataaagcac cttaatggag cagatattat atctgatggt atttcttatg
     3841 gagctataca agtacctggt catggaaaac cgattataat gttatcagat agacagacta
     3901 gtggtggcta tactaagata gggaacgtta taagtgtaga tttgtataag ttagcacaag
     3961 caaagccaaa tgatgtagta aaatttgaac ttgtagatat atatgaagca catagactat
     4021 tgagagagca agaagataag attcaagata tctacaagag tatgaaaaat ataagagttg
     4081 taaaggcaaa agtattaaat gatatagcag tttaatttaa ttttaacaaa tacaattttt
     4141 attataattt ataatataac ttacaaatag ctatttacta tttaccataa ttaagatggt
     4201 ttagattagt caaatagcta tttttttagt ttgagtaaat attttaaatt taaataaata
     4261 cttcaaattt aaaataaaag caatatttaa atctattata ttatctacaa aaagtaggtg
     4321 tgtcatattg agtttattta gattttattg gccaaattgg aggtgatata actccatatc
     4381 cacgagctat tgtatcttta cttggattga tgattttatg tggtacatta gcttttataa
     4441 atgttgagtc acctggattt aatctaacta ccttttcacc catacaaata tcaaccacac
     4501 cactcgttac caccataatt tcttctgatg catgatatat ataatctttg gcatcctcac
     4561 tttctggttt aagttcaaac tctacaaata aaattgaagg tgtaaactca gttgtaggca
     4621 ttggagatac tatctcgtaa aacatttcac tctttggaaa tttcatcata actctatctt
     4681 cttttctaat gactaaatca tcagtattat tgtcatccat aaacatatat aaaggagtgt
     4741 ctaaaacttc agcgattttt ctcaatgaag acaaggatgg ttcaatagca tctctttcta
     4801 tttgagagat ataacttata gataaatctg tcaatttgga aacatcactt aatgtttttt
     4861 tcttgctttt tcttatattt ctcatctttt cacctagcat aagaacctcc aaataataaa
     4921 attaatataa aatattcgct taaaacaaag taattatagc acaacataga agtagtggca
     4981 ataagaatac atagtagatt ttgacaatta aaatttaatt aaatccaaat aaaagtctat
     5041 gaaaatgaat tattgttaaa ttaatacaaa gaaaataata ataaaaaaat ttaatagaag
     5101 agtattaatt gaattaagat tatcacatta tattttggaa aagagtaaat tttcagtaaa
     5161 ttattttgac atatatattg aagtaataat ataatttaaa gtataaaatt ttaaaaattg
     5221 aattataact tcaaaaaata aggagggtaa tatgaagata acagatatca aatttgaaaa
     5281 gcttagaatt aaattaaaaa aacctgtagt agtttcattt ggagtaattg aatatggaga
     5341 aagtgtaatt ttaaaaattg aaactgatga agggtattgt ggatttggcg aagcagcacc
     5401 tcttgcagca gttacaggtg aagttttgga taatgttctt tcagtattgc ttatgtttaa
     5461 aaaagagtta ataggcaaag acccactaga tattgaaacc atacatacca tcatggatgg
     5521 aattattata ggtaatactt ctgcaaaagc agctattgac atagctttat atgatattaa
     5581 aggaaaaatt atgaatgcac ccctttataa agttcttgga ggatttgata gtaaagtcca
     5641 aacggatatt actataagta tagataagcc agaaaaaatg gcagtagaag ctttagaaag
     5701 agtaaaagaa ggttttagga tattgaagct taaagcaggt ataaatcctg aagacgatat
     5761 tgaagctgta aagttaatta gagaagccgt tggagagagt ataagaatta gaattgatgc
     5821 aaatcaagga tggaacgtaa atagcagtat aaatactatc aaaaagttag aagaatttaa
     5881 tgttgatgca attgaacaag cacttccaca ttgggattta gatggtactg cttacataag
     5941 aaataaaagt aacacaaaaa ttatgataga tgaatcatta cattcaccaa tagatgcaat
     6001 taaggctata aaaaagaatg cagttgatac atttaatatt aagctgatga aatcaagtgg
     6061 aatatatcct gctataaaaa tcaacaacat agctgaggca agtggagtaa attgtatgtt
     6121 aggatgtatg ctggaaacga gaattggtat aacagcagca gcaaatttaa tagcatctaa
     6181 aaagaacata acagaagctg accttgatag ttttatgttt tgtgaagaat tagaaggtat
     6241 atctggagga tttgtaatgg atggagatat tatgaatctt gtcaataaac caggtttagg
     6301 aatagaagta aacttatgat aaatttggag gttaaatatg aatacacttt tttcaaatcc
     6361 aggtagtata cttgctgtaa tgacatcaat gattgcattg ggtttttact tacaaagata
     6421 caaagccatt aaaagcttag gaccagcact tacaataatc ataatgggaa taatactttc
     6481 aaacttaaaa gtagttccag taagtactga gttatatgga actatttcaa catatgctat
     6541 tccagtttct atgactataa tgttgatgag tgttgactta aaagaaatga caaaattatc
     6601 aagagaacct ttaattgcaa tttttgtagc tgtattaact gtaagtatca tggcattttt
     6661 atttggatta gtatttgcag aaaaaatatc agaaggttgg aaggttgctg gaatgtttgt
     6721 aggtacatac actggtggaa gtgctaatct tacagctatt ggaacaggtc ttaatgtaag
     6781 tagacaaact cttgcagcag caaatgcagc agactatgta attggagtac caacattgat
     6841 atttatgttt gctctaccag caatattaaa aaattcaaag aagtttaaaa aactttggcc
     6901 atatcatgta gaagaatcag aattagaaga ttgtcaaaat gaagaattta tggagtcaaa
     6961 agaatggagt attaaagata tagcttggat gttggctata ggttttgttg ttacagaagt
     7021 ttcaacaata cttgctggat atttcaattc aagttttagt agtgcagcaa gaatactttt
     7081 agttacaaca atttcaatta taattgctca attaaagcca gtaaagaaac taaagggaaa
     7141 tttagattta ggattatttg ttgcattatt ctttttatgt acaataggtt tttcagtaga
     7201 tataaaagag tttttaggtt caacttttac tataacactt tactgtttta gcataatttt
     7261 tgcttcattt gtttttcatt taggcataac tagactttta aagataaagt atcaatatgt
     7321 aatactttct atagtaggag caattgctga tggtcctact tcagctttag tagctgcttc
     7381 tgcaaagtgg aattcgcttg taagtgtagc agtcgtaatg ggagtaattg gtggagtatt
     7441 aggaaattat gcaggtatat cagtggcata tgcaataaat atgtctctag gattataggg
     7501 gggagtttaa ttgataaaat ttaaattata catatctgga ctatacagtg gaaatgtaat
     7561 ctttgatgga gatttattaa ttgaaaaatt gaatcctttt acaaataaaa tagaaagttt
     7621 aaaaccaata agcaaagaag aaaatacata ctatttaaat cttacaaaaa tagatttaaa
     7681 gtctttattt aataattttg atgtatatac taaaagctta gtaaacacag ataaagacac
     7741 agttataaat aacttaggag aaacgcattc taaacttaat gaatatatat gggttcaaag
     7801 aaacaaaaag ttcccactag atataattat tgtagataat aaaatagtag gttttatctg
     7861 tttatccaga gaaacttgta ctatattaat tatggatgga tatgaagagt atactgtatt
     7921 aaaagaatgg gaaaaaactc ataaaaatga agagatatat tctataagat ttggtggaaa
     7981 ttatatgatt gatatgaaag atggtataaa actttctaca gatgtatatc tccctgattt
     8041 tgtagattct gccaaaaaag caccgacaat tcttatgaga actccatatg gaaaagaaaa
     8101 tgataaagaa atatattata aatatgtaca aagaggatat gctgttgtaa tacaagatgt
     8161 taggggaaga aatgaatcag aaggtaagtg ggaacctatg attcatgaga gggaagatgg
     8221 agatagtacc ataaattgga tagtatcaca ggaatggtca agtggaatag ttggaatgct
     8281 tggagcatca tatcttggtt atgtccaatg ggcagctgca tcttcaggca ataagcatct
     8341 taaagcttta gttagtatag taacatcagg aagtcctttt attgatatac caagaaaagg
     8401 aggagctttt gtatctggta tgcttgcatg ggcatttatg gtatcaagaa ataaagttga
     8461 tagaagcaaa atggtaagag atgattggga tgatgtttta aacatacgac caatagagaa
     8521 tatacctgtt gaagctcttg gatatagaat tgaatttctg gaagaatggt taaagagagt
     8581 agaaaaagat gagtattggg atttgatgga ttggcattta caaaaagata aaataaatgt
     8641 acctgcattg gttgtatctg ggtggtatga tgataatagt atgggaacta cagaggcctt
     8701 agatgttata aaggactatg aaaaaggaaa gagaaaggct attctaggtc catggatgca
     8761 taattcaaat actttaagag atataaatgg tatttcttta ggaaatagtt cattaagata
     8821 tgatttggat tataattatc tattatggtt tgataaatac ttaaaaggta tagaaaataa
     8881 catagatacg actgctccag tagagtatta ctctgtaggt tctaataagt ggaagacaga
     8941 agaaaattgg ccaataataa ataagataga taaaagtatg tatctaataa gtgatggaaa
     9001 tgctaacaca tctttaggaa atggtagatt agtatttgat aatgatttag aagaaaagta
     9061 tgatagttat atatataatc caaaagaccc atcagtacaa ctaattgata tgtcagagaa
     9121 tgaagttgga gttccaaata actacaaaga tttagaaaaa agaagtgata tgttgtgtta
     9181 tacatcagat gcttttagtg aagaatttac tgtaacaggt gatataaagt tagagttttt
     9241 tgcatcaagt tctgccaaag atactgactg ggttataaaa attatggacg tggatttaga
     9301 tggaaattca attaaattag ctgatggtat acttagtgct agatttagaa atagttttta
     9361 taagtcagag tttatggaag aaggagagat ttataagttt acggttataa cttcaaaaat
     9421 atcaaacacg tttaaagttg gtcataaaat tagactagat ataacatcta gtgctaaaaa
     9481 ctttattttc caaaatagca acacgacaga gggatataat agtattgaat atatagaagc
     9541 aaagaatacg atttaccatg gaggtaaata tccatctaaa ttgatattgc caatagagaa
     9601 taagtaaatt tatatagatt aaatagtgag gaggtgtctc aaaatgaatt ttttagttcg
     9661 tgagacactt tcttttatac gaaatcaaat atattttcat catttcaaca atgaaaaaga
     9721 ggcttatctc tctgttttga gatagcctcc tataagctaa attgattatt ttgtagctgc
     9781 aacaacgctc atttcaacta acaaatgctc tctagcaagt ctagcttcaa cacaagctct
     9841 agcaggttca aacccttttt caacccaagc atcccaaaca gagttcatag cttcaaagtc
     9901 tttcatatct cttaaataaa tagtaacaga tagcaaatgt tgcttatctg aaccatattt
     9961 gtttaataaa tcttctattt tagctaaaac ttcagttgtt tgttcaatta ctcctccttc
    10021 agcatgagtt tgtccacata aataaactgt attgttatga actacagttc tactcattct
    10081 tccagttcct tcatatctct ttatttccat attttaaata cctccatagt taaaaaatct
    10141 attaaaaatc taaataataa attaaaaaaa gtctatacta tataaaatta tattatatat
    10201 tctgataata atccataaaa acatataact ttttagctat aattattatt gttattaatt
    10261 gaaaagattt attagttact tgacatagtt tacactttta attatagtat aaattggaat
    10321 atttagataa ttattattta atgtataagc taagttttta attaatatgg ctttatacat
    10381 ttatatagaa aagaggtacg tatgagtagc acaatcaaat caaatcgaat aaaaggtata
    10441 atgtttataa tagcatcagc gcttggcttt gcaatgatgt ctgcattcgt aaagctagct
    10501 ggagatttac caagttttca aaaagtgttc tttagaaatc tggtatcagc tataatagct
    10561 ttttggctta tcataaaaca taaaggaagt ctaactggta aaaaagaaaa tttaaaagta
    10621 cttttatata ggtcaatatt tggaactcta ggagttatat ttaactacta tgcaattgac
    10681 aagttgatgc tttcagatgc taatatgcta aataaaatga gtcccttcct tgtagtaata
    10741 ttttgtgcaa ttgctctaaa agaaaaaata aatgtaaagc agataggagc tattataatt
    10801 gcatttatag gagctttatt cataataaaa ccaacattta gtgtagaagt aattccatat
    10861 cttggaggaa ttgcaggagc tgtatttgca gcaatggcat atacttgttt aagagttctt
    10921 ggagatagag aagattacta tacaattgta tttttctttt cagttttttc attagttact
    10981 gtagggccaa ttgcttttgc agtatatgaa ccaatgaccc ttatgcaact tatatatcta
    11041 ttactaggtg gagtatttgc aagtctaggt caatttggaa taactctagc atataaatat
    11101 gcacctgcta aggaaatctc tatatttgac tatagtaata taatcttttc agcaatttta
    11161 agtatattct tattcaatgt ttatccagat atgttaagtg ttgttggata tttaatagta
    11221 ttttcggcag cattttatat gtttttatac aataagaagt tagataaatt agacaaagaa
    11281 aagaagaagt aaactagata aaagtgtttt tataatttgt attgaatatt taagtctttg
    11341 tattaaaatt aaagaaaatt taattaaaaa tgtttggtat attaacttat aacagcagtt
    11401 aaaattgaaa aattataact gctgtttctt ttattaaaaa tatccaaatg tctgtaaaaa
    11461 tagactataa tagagtagta taaataatat aaaggggata aattaaagta tggaaaaagt
    11521 gaaagttgct gtgattggag caggtaatag aggaacgtat gcatatgctc catatattta
    11581 tgaaaattct gatgtatgtg aaatagttgc tgtagcagaa cctaaaaaag gaagacgaga
    11641 actatttaca caaaaatata acctagatag taaaaatgta tttgaaactt tagaagactt
    11701 ttttaaacat gacaagatgg cagatgcagt aataatagct acaaatgatg atagacatta
    11761 tgatgtagca aaactagctc ttgaaaaagg ttatcatgta ctattagaaa agccaatgtc
    11821 taatagttta gatggtttgg tacatataga tgacttgtgt gataagtata aagataagat
    11881 ttttatgatt tgccatgtac tgagatattc tccattttat aataaattaa aagaaattgt
    11941 agaaagcaaa aaattaggag agctagttag tatacaatat aatgaaaata taggatattg
    12001 gcattttgca catagcttta ctagagggaa ttggagaaat agcaatgaaa ccagcccttt
    12061 aattttagca aagagttgtc atgacatgga tattttgtta tatttagtag gaagtagatg
    12121 caaaaaaata tctgcatttg gaagcttaaa gcatcttaat aaccaaaatg ctagtggaga
    12181 gatggcacaa aattgtcttc agtgtttagt tgaaaaaaaa tgtccatatt cagcaaagag
    12241 aatttattta gaaaaagata gaagtataaa tagagctgtt cacataaatc caactgaaga
    12301 gaatctatta aatatactta agacaagtcc atatggaaga tgtgtttaca gatgtgataa
    12361 taatgttgta gacaatatgg taaatatact acagtttgaa aatggagtga cggctacttt
    12421 taatttatgt gcatttacta aagaaaatgg aagaactata aagttaatgt ttagccatgg
    12481 tgaagttggt ggggatttaa ataaaaatga gataagaatt aaagaatttg gaaaaaacga
    12541 agaaatagtt atgaatccat caaatcaaca gaatatggta gaggaatatg atagaaatct
    12601 tatagctgaa tttattaaat tagtttcaaa taaagaacta gaaaagggta gagtagcagc
    12661 aaaagaagct atacaaagtc atgtaatggc atttgcagca gaatattcta gggtcagtga
    12721 tgaagtagtg tatatagaag aattttttga tagtgctaag caaatgacta aggagataga
    12781 agaaactata ttttaattaa taaaaattta ttaaataaaa taaagataga ttaaataata
    12841 gaaatacgaa aatatgtgtt ctattaagga atctatcttt tttattaagg ttttttctta
    12901 tccctatcaa aaaaatccat acattctgat aaaagataag aaagtaaagc gaataagatt
    12961 tctgtactta aaggaatcgt tactgaatcc ataaaggtat caattatgtt catagctaga
    13021 aaagttagta taaaatcaat tataaattcg caaagtttat atattgagta tggtaatttg
    13081 tttacatatc tgaatatatc taaaatagta gtgcatagaa aatcaattgg agtggttata
    13141 caaaaatata ttaaaaagaa aattaataca gctccaagag atttatattc aaatcctaag
    13201 aatgataaga agccaattcc tataaccgtg aaaatacaaa aagttatacc aaaaaaaaca
    13261 gcaaaaaata aaaatacaga aataaattcg cctaaaggaa tccttctttt attacttatc
    13321 ttaatcaact ccttaattta tgatatgaaa tttatataaa taataacatt cttaaaaatt
    13381 cataatagtc ctctgtttca aatagtactg tttgagaatc tatttttttc atatttggat
    13441 aaaaagaggc cttaaaagct ttataatgtt ctctaaattt tatttctatc ttaaatttat
    13501 ctggcaattt tattaagtgc ctagataaat ctccagataa agcttttttg acaccttttt
    13561 caattttttt tagtgcaagg tttggatgga ttgaaattga accatttcca attccttttg
    13621 acacaggaac agtgactata tttggattta gtttttttgc actctcacaa agcatttcat
    13681 ctccacttaa aaatgctaca ggaacattat aatatgcagc aatataagca tttatagtaa
    13741 attcagatgc tatttcatca tttattttaa aatagtcata atcaatatcc atagtatggg
    13801 aaagtggatt accattacaa gtagctccag agtgatagcc agtgaatata catgcatcaa
    13861 agctttcatc aattccagcc atcataacta gtgggtcttt ggtccatcct ctaagtattt
    13921 tgacgttttc aggtaaaata tttggattaa tatttctacc actgtcatgt gcatctttaa
    13981 caagtatttc atgtacacca gattgagtag ctccatcaca tgctgctgtt atctctttac
    14041 tcatttgtgt tctatagtgt tgcgaaaatt cacattcaag ttttgtttca tcccaattta
    14101 caataccaca agtaccttct atatcagcac ttaaatacaa tttcataata tataccccct
    14161 tatgtttatt attgatgata ataacagata acagaaaata cataatcaat aaagggcata
    14221 aaaatattat gtcttactat taactattat taaatctgta actgaaaaat aactaaattg
    14281 gtataaaaaa atataacact atgtgtcatt tatttatata gttttttata tctgatgttt
    14341 atataaaatt gtaacatata agtggttaca tttactagtt taacatagtt aaaatagtta
    14401 ggtattattt ttaagttaaa ttgattatta taaaatcgca gaataaaaga aaaatttaat
    14461 ttattaaaat gcatgaaatg ttttttcctc aacataatgc taaattaaac tcaaaaagaa
    14521 ataatagaaa aatcttatgt agcttagaaa gaatgtgaat gatttaaggg aaaaatagaa
    14581 atttattata taatattagg ctgtattata taatacagcc taatattata tgtatatgtg
    14641 acataaaatc ttggtaagtg taaataaaat aaatgtggaa aacaagttaa tgaaatcaag
    14701 aaaaaagtaa tttaaaattc aatttcatct tcccaattaa attctcctga tttttctaca
    14761 ctcttaggcc agtgactaca ccatttagga acggacggat tttcaaccct atcaacgctt
    14821 ggagtatatg gctttaagtc aataacagga ctattatcat tagcatcaat atatggtatt
    14881 tttattatgc cattttcatg gtcgatattg ataatttgta cagcagtcaa tgctattgga
    14941 ttgggtctta taggtgacct tgttgcaaat attcctataa cgtctggtcc acttttataa
    15001 ggttttgggg tttctaaaat acttcttagt tcaggatcat caaacccatc agcccaccaa
    15061 aatacattta aatgactgaa atcgtcgagt tcatttaatg caggtacata ctttttgtct
    15121 aatttaataa agacctcttc gccttgaata catattttac ctataggatt tattaatatt
    15181 tctttcataa tattacctcc aactatttaa tttatcattt gtaattttag ggtataacat
    15241 gacatcatgt gagggtcaat ataaaataat aaataaaaat attaatagtt agaatgataa
    15301 tacatttttt acatggagca tgacaccagt aataaggcaa tcttcgtcaa tatcaaaagt
    15361 agaggtatgc aaaggggaag taatgttttt ttcacgattt gcacaaccaa ggtggtaaaa
    15421 agctccttca cagtgtctta gaaaatacga aaagtcttca gccccaagag tgggcgagtc
    15481 tttaaggata atattttctt caccaaacaa ttctacagca ttttgtttga cgtaatctac
    15541 tagatggttt gaattgataa gagctggata accttcctca atttcaacag aaatacttcc
    15601 tccaaatgtt ttacaagtaa aatcgcaaat ttctctaatt cgtttttttg aaaactctct
    15661 agtttcagga gtcaaggttc tcaaagtacc ccttataacc actttatcac aaactatatt
    15721 ttccttgatt ccaccttcaa tctttcctag agaaataacc acagaatcag ttggatttgt
    15781 gtttcgacta acgattgtct gtagagctgt aataatatga gcagatataa caatagcatc
    15841 aataccttga tgtggataag ctccatgaca ttttgaacca tgtacagtta attgaagtgt
    15901 atcggtggaa gcatttagag tgttgtattt taattcaatt agatttttgt tgatattggg
    15961 atttacatga agtccaaata tatattttac atttgggttt tcaagacatc catcttctat
    16021 cataagttga gccccaccaa tagtttcctc ggcaggttga aaaaagaatt ttacatttac
    16081 atttaagtaa tcctttaatt taaatagtat attacaagta cccagtaaaa tagcagtgtg
    16141 tgcatcatga ccacatgcat gcattttgcc aatattagta gatttataag aataattaag
    16201 ttcttctgtt ataggtaagg catctatatc agctcttata gcaactgttt caaagtttgg
    16261 actcacgtta atgtatgcag ttatccctgt atgattctta tattcaatat aatttatacc
    16321 tatttcattt aagtatttaa taattttttt cttagtttga aattcttcta atccaagttc
    16381 tggagtctta tgtaaatctc ttcttatatt tataacccaa tcatttatct catgacaaag
    16441 agtttttata ttaagcataa aaaacatcct aactatatta aaaatttatt tttcttcatt
    16501 aattatatta ttttatgtaa atcattatta tgtatcaagt acaaataaaa tatcttataa
    16561 ttatattaga gatatttagt atggagaaga atatatcatt ttataagaag aggtgtttaa
    16621 atgttaggaa taattgcgcc atctgggcca ttaagaaata caagtttgga ggaaataaag
    16681 tttaatttag aaagttatgg ttacgaagtt aagttttctg aaagttgtag tttaaattat
    16741 aaaggttatt tagctggtaa tgatgatatt agagcaaggg atatagaaga tatgttttta
    16801 gataaagatg tagatataat tatgtgctta agaggaggtt atggaacaac aagaatattg
    16861 gataaaataa attatgatat aataaagcaa aatccaaaac catttatagg tttttctgat
    16921 ataacaggtt taaatttagc tttctataaa aattgtggtc ttttaccata tcatggaata
    16981 atggcagcaa atgtaggtaa atgggataaa tttacctata aatcattggt aaatgcttta
    17041 gaatttaaag atgaattata tttagaaaat ccaaaagaag aaaagattta tactgtatgt
    17101 gaaggtaaag cagagggaat aataatggga ggaaatttat ccttaataat tgccacaatg
    17161 ggaacaaaat atgaaataga tacaaaagat aaaattttgt ttattgagga aattggagaa
    17221 cctcaatata aattggatag aatgttaaca caactatatt cttcaggaaa acttgaggaa
    17281 tgtaatggta taatttttgg agattttaag gattgtattg aagaaaatga tttgatggaa
    17341 ttactaatag aatttgcaaa taaggtcaat aaaccttcta tatataatct tcagtcagga
    17401 cattgtatcc caatgataac tattcctttg ggcagaatgt gcgaattaga tgctacagaa
    17461 aaaatcgtta aattaaaaaa atagtgtgta tataaatata atagaaatat ggtataatct
    17521 tatacaagta aaaatttagc taggaggatg aaaaatgtct gatgatagaa taatagactt
    17581 taacgaacta aagaacaaag ttaaagattc tgatgtagat aaatttgagc aatacatata
    17641 taacttatat ttttctgtta tggatggaaa gatgtctatg gctgagtttt caagaaaaat
    17701 attcgattat atgagagata acaacatatc tcaagaaaaa tttatgaaaa tacaaaagca
    17761 atttatggag agatatggta tggacactga agaagtagaa aaacaactta gaaactttgg
    17821 aatcgaccct tctacagcag gttttatgag taataatact tccagcaaag tatctacaga
    17881 agatttagag tcatttaaga agagtgcagg attttatgag aaatatggag aaaaaatcca
    17941 accaaagagt tgtataacaa catttataaa aaatgactta aatgatatta atgtaataat
    18001 cgaccaagaa aagataatgc tttgtagtga tagaaaaata aatcttatgg attcagaact
    18061 aaatgagttt ttattagaat ataagaatat gtttaataaa aaaataaagg tagtaatgtg
    18121 cgaaactacg aataagtatg attattaaaa aatgaattta aatactgaag tagtataaat
    18181 taattgtact acttaaaaat aaaaatcgca atttattatt ccttataaat ttgtataagg
    18241 aatatctaaa ctgcgatttt tt
//
LOCUS       DAGSWI010000081        18114 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.081, whole genome shotgun sequence.
ACCESSION   DAGSWI010000081 DAGSWI010000000
VERSION     DAGSWI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18114
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.081"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            15..572
                     /locus_tag="MJF80_002480"
     CDS             15..572
                     /locus_tag="MJF80_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0281135.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(811..1671)
                     /locus_tag="MJF80_002481"
     CDS             complement(811..1671)
                     /locus_tag="MJF80_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBZ0281136.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            2164..3567
                     /locus_tag="MJF80_002482"
     CDS             2164..3567
                     /locus_tag="MJF80_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0281137.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            3786..6263
                     /locus_tag="MJF80_002483"
     CDS             3786..6263
                     /locus_tag="MJF80_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBZ0281138.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRIYPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIETFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIIGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            6278..6964
                     /locus_tag="MJF80_002484"
     CDS             6278..6964
                     /locus_tag="MJF80_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281139.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            7238..8071
                     /locus_tag="MJF80_002485"
     CDS             7238..8071
                     /locus_tag="MJF80_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281140.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            8109..8564
                     /locus_tag="MJF80_002486"
     CDS             8109..8564
                     /locus_tag="MJF80_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBZ0281141.1"
                     /translation="MKFGGVLLIVKDINKSKKFFEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFIIQFQ"
     gene            8601..8840
                     /locus_tag="MJF80_002487"
                     /pseudo
     CDS             8601..8840
                     /locus_tag="MJF80_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
     gene            9301..9621
                     /locus_tag="MJF80_002488"
     CDS             9301..9621
                     /locus_tag="MJF80_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBZ0281142.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            9759..10403
                     /gene="trmB"
                     /locus_tag="MJF80_002489"
     CDS             9759..10403
                     /gene="trmB"
                     /locus_tag="MJF80_002489"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBZ0281143.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            10532..11278
                     /locus_tag="MJF80_002490"
     CDS             10532..11278
                     /locus_tag="MJF80_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281144.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEV
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGKAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(11407..11685)
                     /locus_tag="MJF80_002491"
     CDS             complement(11407..11685)
                     /locus_tag="MJF80_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281145.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     ILVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            11960..12406
                     /locus_tag="MJF80_002492"
     CDS             11960..12406
                     /locus_tag="MJF80_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281146.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            12676..13128
                     /gene="accB"
                     /locus_tag="MJF80_002493"
     CDS             12676..13128
                     /gene="accB"
                     /locus_tag="MJF80_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBZ0281147.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            13144..14505
                     /gene="accC"
                     /locus_tag="MJF80_002494"
     CDS             13144..14505
                     /gene="accC"
                     /locus_tag="MJF80_002494"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBZ0281148.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            14520..15374
                     /locus_tag="MJF80_002495"
     CDS             14520..15374
                     /locus_tag="MJF80_002495"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBZ0281149.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            15395..16339
                     /locus_tag="MJF80_002496"
     CDS             15395..16339
                     /locus_tag="MJF80_002496"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBZ0281150.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            16528..16788
                     /locus_tag="MJF80_002497"
     CDS             16528..16788
                     /locus_tag="MJF80_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBZ0281151.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            16941..17312
                     /locus_tag="MJF80_002498"
     CDS             16941..17312
                     /locus_tag="MJF80_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBZ0281152.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            17342..>18114
                     /locus_tag="MJF80_002499"
     CDS             17342..>18114
                     /locus_tag="MJF80_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281153.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIG"
BASE COUNT         6998 a         1954 c         2876 g         6286 t
ORIGIN      
        1 aagggggatt agaaatgtct gattctgaat atcctcaaat gaaaacctta gaaactagaa
       61 attgtatact aagacctgca tcattagatg atgttgaaga tttttttgac tgttataaaa
      121 ataaaatagt agtaaaacat ttaccattta gtgagcataa atctttagaa gatacaagaa
      181 agtttataaa atcatttttt ctaaatccat ataaaaaagg gaaaattggc cactttgcta
      241 tagtatataa aagagacaat aaagttattg ggaatatggg gtttaataat ataaatccta
      301 aagcattaga agcagaaata ggtatatgta taaatccaag ctattgggga catgattttg
      361 ctactgagat aacaaaaaga gtaatacagt atggatttag agaactaaac ttaaataaaa
      421 taattgcaat aacctatgaa gaaaaccaaa attctacaaa atcattagat ttattaggat
      481 ttaaatgtac aggaaagtat atcaaaaaaa ttcatactgg aaatactgtg aaaaatgttc
      541 cttgttatca atatgaattg aaaaaacaat agatataatc atttaatatc tatgctggtt
      601 atttagtaat gattgttttc taaataaaat ttcaataaag tggttgcctc aaaatataga
      661 taatcctgtt tttattgtta aaatactgaa aatgcatttg attttataca aaaaagagtg
      721 tctcatgaac taaaaagttt atgagacact ctttttgaat aaatcgtatt ttaattaaaa
      781 tagtatatat acttaaaata ttaattttta ttagttctct aaatttttaa atccttctcc
      841 aagaacctca tgtacatctg taacaataac aaatgcagta gggtcagttc ttttaactaa
      901 tctctttaga taaagttctt gttttttaga aactacgact aatagaactt ctttattttc
      961 tctagtgtat ccacctttac catcaagaat agtaagacct ctatcaagtt cttcagttat
     1021 agcttgtctt aaactttcag gctcatcaga gataataaaa aatgcttttg aatggtcaaa
     1081 tccttccatt atcatatctg ctatttttat taatacacaa agtgctatac cagagtaaag
     1141 agcagtttct aaactacgat ttactatacc agaagatata acaaccattc catctaggca
     1201 tgacatgaat tttgtagctt ttattcctgg gaatttctta ctaagtatga gtgcaattaa
     1261 gtctgttcct ccagtagaag catctgattt aaacattatt cctaaaccta taccaactag
     1321 gattcctcct gcaatagaag acaataataa gtcattagta aatcttagtt ttgataatgg
     1381 gtctgttaat tgaactatag ctgaaaatac aactgttcca aataaagttt ttaatgaatt
     1441 ttttgtaccc ataattctaa aagcaagtat tactaatggg acacctataa ttaacatgat
     1501 agctgatact ggaagtcctg taaccttgtt tagtactaat gatagaccac taagtcctcc
     1561 aggagcaatt gtatgtggtt ttaaaaacat atttatacca atactcattg atatacaacc
     1621 aaaaaatagt ccaagcattt caagtaaaat tggcgacttt tgttttttca tataaacacc
     1681 tccatgaact tgtcacaatt taatattata acatatttga ccagtttata ttatataaaa
     1741 tttcttgata taagcaattt caagtatttc attattctta atatttttga atttttatat
     1801 agtgttttca tatttttact gatgttgtta ctctcctatg aagtttttat atattataat
     1861 ttttttattc ttttataaag tatcccctat aaagatggtt ctatgaaaat tttctctata
     1921 ctgtttaacg tatatagaga ttttgtgtat aattataagg tatttaatgg aaaaatttat
     1981 cttactttta tatgttatca aaaggattta ttaaatacaa cagatagctt gataacatat
     2041 aattatataa atataattta tgtagatagt tgtattttta aataaatttt tacatatcaa
     2101 taattattgt ctattttata taagcaataa tcaaaataaa agtttaagaa aagagttgat
     2161 taaatgcata aacctagtta taaaacagta tttcattcat acattatttt tttcatgatt
     2221 tttatttgct taatcctgac agtaataggt attttcttat cacttattac tattcagaaa
     2281 ccaaatgggg aatttattcg tagtgactgg ccgaaaaaat ttacagaaaa ctttaatgaa
     2341 caaatcgtat ttatagataa taagccacaa gtaaaacagt ctggattaga aaaacttcga
     2401 gaaaatcaat tgtggttgca aattttagat tctagtggaa atagagttta tgggttttta
     2461 gaaccaaaag agcataaaga ttattattct aattctgaat tattagcatt agctaaccaa
     2521 aataattcaa gtaatggaac tgtatacttt aatacaatta aaaataataa aaatgactat
     2581 atttatatta tatattttcc attaaatata tctaaagtga atatgttttt tgatggaggt
     2641 aaatttatag gtggtaagtc aattgtattt tcaatcatag ggattatatt tttaataatt
     2701 ttggtttctg gaattgctta tggttactgg gtaacaaagt gcatatcaaa tattacatct
     2761 gctgttagcg atatttcaaa acgaagctat cttccagtta atattaaagg tgtttttgct
     2821 gatatatata atagtttaaa tattttaaat gaagaaattc atagaagtga tgagatacaa
     2881 aataaaactg atattatgag agaagagtgg atttcaaata taactcatga tttaaaaact
     2941 ccactttcac caataaaagg gtatgcagag cttcttacag atagtagtac taattattca
     3001 cctgaaaaag tatataaata tgcttcaatc atgttaaaga atatagaata tacaaataca
     3061 cttataaacg atttaaaatt aacctatcaa ttaaaaaatg gaataatccc tttaaataaa
     3121 aataattatg atttagtaag atttttgaaa gaattatcca ttgatatttt aaactatcca
     3181 gaatatgaat caaatatagt tgaatttaag agtaatatag ataatattaa ctttgatttt
     3241 gattgtacac tattaaaacg agcttttagt aatcttattg tcaatgcttt tacatatgga
     3301 gataaaaata ctaaagtttg tgtacatata acattaaatg aaagaattga aatcattata
     3361 tctgataatg gaaaagggat gtcaagagaa gaagcatcaa atttgtttaa tcgttattat
     3421 agaggaagca atacaaaaca aaaaatagaa ggaactggtt taggactagc aataacgaag
     3481 caaattattg aagttcatgg agggaatata tctgttgaaa gtatacctgg attaggtagc
     3541 tcattttata tcacctttcc tatttaatta aggttaatta aggtcaaata aaaaatatat
     3601 taaggttgat agattattat atttatatag tagtttatca atctttttta tttaaggggt
     3661 gatttgatta aaaatattat aaatagaatg ataaaagtat ggaggagtct attagagatt
     3721 taatttagat taaagtataa aaatattaga ttgaaatatc ttagataagg aaagaaggtg
     3781 taaatttgaa aattatatta aagtatattt tcacaaatat taaagaacga aaaattagaa
     3841 cagcagttat gttattgtcc atagttttat caacagtttt actttttgtt tcattctcaa
     3901 ttggattatc ttatgaaagt gcacaaagaa aaatggcaaa aggtatgtat ggtacagcga
     3961 ctatatctgt ccaaagtaaa aatccagata tattaaccaa tttagaagac atacctgatt
     4021 taaatactat aaaatcaaag gttggtgtat tggaaagctc tgctatttat aataaagggg
     4081 gatactatga agaatttagt cttatatcag cagacttaag tcaactaaat aaaatcaaca
     4141 agccacgatt agaaaatggc gatagtatta ctgatttttc tggtgataaa attattcttc
     4201 caaatcgctt tacatcaaaa tataaaataa aaaaaggtga ttctattact ttacagattt
     4261 atggaaagtc ctatactttt caagttagtg atattgcatc ttatgataca gtatttttga
     4321 ggaacacaag aggtgtaaat gcactgctac ccaaagagac tctatcaaaa attataaata
     4381 aaggcagtgg atatacaaga gttcttatag agtcagaaga agaaattaca gaaaatctag
     4441 taaataaatt atccgaagaa ctttctactg aaaaatatat ggtatcaaat actataaatg
     4501 aaactaagat aatttcagat gctagacaaa agacaatgcc tttcttttta ataagttttt
     4561 ttgcattaac ccttagtata tttataatat acagcagtta taaggttatt acattagaac
     4621 gtcttccttt tattggaaca tttagaagta ttggtgccaa tgaaaaaact gtaactcgta
     4681 tattgatgct tgaaagtata ttatacggaa gtataggagg attaattgct attcctattg
     4741 gtgtagttgt tttaaattta atgcttcatg ggttaggaaa ttctctcgaa caaggaatat
     4801 caattccagt agtaatatca cccattggag tgattatatc tgttatagta gcaataatcg
     4861 tttcttcatt tagtgcatat atacctgtta aaaaagctag ccatttgcca ataaaaaata
     4921 ttgtactagg aacagtagaa gaaaagaatg tttcaaatcg ttctatactt tttataggaa
     4981 gtataatgtt tattttatct attttacttc caagaatata ccctgaaaat acattgtatc
     5041 tagctggtgg attttcatta ctaggattaa ttgtagctac tattgtgttg attcctctta
     5101 ttactgatat catgtctata gtttttgagt ttgtttataa aaatatttta gggaatgagg
     5161 gtaaattagc tgctagaaat atgaaaaata acaaaaatat aatacagaat atcactttac
     5221 tatttataag tatttctgca gttattgcaa taagtgttgt tggtaacttt gtcaaaactt
     5281 atataacaga tgtgtttcgt gacgcagaat tacaaggttt tgcagatgga aagatgaatg
     5341 aagagtttat tgaagatgtt agacatatgg atggaattaa aaaaatatta cctctttatg
     5401 ttatgaataa tgagatttct ggtaatggtg taacattatc aagactagag ggtacagata
     5461 atataaaact atataactct atgtttggaa tcaattatac aaattttgag ataaaaaaac
     5521 aagtaataga aacttttaat gataaacgtt ctgttatttt gaatgaagat actctaaaaa
     5581 aagtaggctt atctatagga gatacaataa ctctatctaa tgataagtat gatttttctt
     5641 ataaaataat aggtagtttt aaatcccgtg caaatgatgt agaagctgtt ataccatcac
     5701 attatgcagt taatgatttt gataaaacta attatggttt tttagtatat acagcagtca
     5761 atccagatgc tgttatgatt cagataagat atttatttgg agatacatat aattggagtc
     5821 gtacagttga agaatttaat aatgattctt taaatactat aagtagtttt ttgtctccta
     5881 tgaataagat gacctatttc atttttcttc ttgctacagt aggtattatt aataatctac
     5941 tgattaatta tattcaaaaa agacgttcta tagctatgta taaatctata ggactaagta
     6001 ataaacaaaa tataaaagtg acattgattg aagggtttac ttcaggttta cttggagctg
     6061 ttattggtat tgtgatatct atacttgaga ttcaaactat ctttatagtt gcaggaccaa
     6121 aaatttcaat gaaaccagat ttagatttta aaacatttat tattgttggg ttattgggaa
     6181 ttatagtaac tttaataggt tcgatagtgc ctattataaa agggaagaaa atgaaactga
     6241 tagaagaaat taagtttgaa taattatgga ggttaatatg aaaattacac ataatagttt
     6301 agctgtagaa gcaaaaaata ttatcaaaga atataaaatt ggaaatacaa caactcgagt
     6361 gttaaaagag gtatctttac aagtaatgaa aggtgaattt gtttctatca tgggtcaatc
     6421 tggttctggt aaaagtactc ttttatatat tttaggtggt ttagatactc caacgagcgg
     6481 aaaagtgtat atgaatggag ctgatatatc acattttaat gatgagaaaa tgagcataat
     6541 acgtcgtaga aatataggat tcgtatttca attttataac ttaataccaa atttaaatgt
     6601 ggaggaaaat ataatgcttc ctttgttatt agatggtaaa aatttaaaag attataaaaa
     6661 tcaattagat gaaatcttag atatagttgg tttaactgat agacgtaaac atacaccacg
     6721 agagttatct ggtggtcagc aacaacgtgt tgctatagct cgagctttaa ttggaaaacc
     6781 agagatttta tttgctgatg agcctactgg aaatttagat agtaaaacag ggatagaaat
     6841 aatagattta ctgaataaaa taaacagaga caatggacaa actataatta tggtaactca
     6901 ctcaccagaa gcagccaaaa gtagtagtcg tactattaca gtttcagatg gtttaattgt
     6961 ataaggacta attattgtat ttgagataag catttttaac tatataaatt agacatatgt
     7021 aaagtggata taagttaaat actataaatg tgctataata ttaaaataac agataaaagt
     7081 actatatttt ttattgatta ttttaatttt atttgtttat taaatatcta atgagtattc
     7141 atactatctc tattattaaa agcagttaat gatatataga gataatatgg attcttctta
     7201 ctttaataaa tattcacgat aggacaggat ataatatatg ttaaaaaaag aaagtattca
     7261 ttttataaat aaaaataggt cacttatagt ttgtgaagac aattttgttt atctcttgcc
     7321 ttgtatacaa ttaagaaatt ggatatcgaa ttttactata tcgtttccaa atcaaacaat
     7381 aatttctgat aattacacga ttattcctca tggaagtgta actttggtgt ttttttatga
     7441 tgccacaggg ttacatagtc tattatttgg tccaactact aaaccaaaaa cagtaggaaa
     7501 tattgctaat agatgtgatg ttatactaat tatagaattt caacctgctg gttttttccc
     7561 gcttataggt atacagcaaa gtgaacttat tgataaagtt gttccatttt ctataattaa
     7621 tacttcatta gatttagaaa ttaaaaaaat atttaatgaa tctttatcta tagataaatt
     7681 gattttaaag ttggaagaat tattgatttc taatattaaa attgaatact catatgagtt
     7741 tattttggca attcagttga ttattcaaaa ctctggaaac atatcttcac aagaaatttc
     7801 taaaaaagta ttttatagtt ctaggcattt aaacagactc tttaatcagt acctagggtt
     7861 gagtatgaag tcattttctc gtttggtacg tattaataaa tctataaaac ttctaaacaa
     7921 taataaaact tcattgatgt ctatatgtaa tgaattagga ttctatgata taccacattt
     7981 tattaaagat tttaaaattg tctgtggaat aactcctcaa aaataccgag ctaatatgtc
     8041 tgatttttac agtgaaattg caaaatttta agttataata cttataacaa agaaaggata
     8101 gtgataagat gaaatttgga ggagtattgc taattgtcaa agacataaat aaatcaaaaa
     8161 agttttttga agatgttatg gagcaaaaaa tcacattaga tttaggagaa catgtatcat
     8221 ttgaaaatgg atttggatta caaagtaatt ataaagctat aattggatat gatttaccaa
     8281 tatatcaaca atcaaataac tttcagttgt attttgaggt taatgacatc aaacaatggg
     8341 aaagtaagat aaatagaata ggaaatattg aatttttgca ttatataaaa gaatacccat
     8401 ggggtcaaag aacttttagg ttttatgatt ttgataaaaa tattatagaa atttcagaaa
     8461 gtatggaaag cgtcataaag aggcttttaa aacaaggttt agctttagaa gaaatttcaa
     8521 aacgcactat gtatccagtt gaatttatta tacaatttca ataattataa aatattaaaa
     8581 tatataattt tttctttata ttgcaaaaaa caaaggcttg gggcataata gaaccagaaa
     8641 aacctgaatc tcaacagcaa atggctcata tggatttcgc tgttaatgat ttagaagaag
     8701 cagttcaata tgcaattcat tgtggagcta caatcgcaga ggagcaattt actgatgact
     8761 ggagagttat gattgaccca gctggacacc atttttgctt atgtcagatg aaatcaatta
     8821 tggaaagtag ccatttttca ttttatagac caaaatattg tttattaatt acaaaaaagc
     8881 acaacagtat ataccattgt gcttttactt attatacatg aatacactaa aaaatataaa
     8941 tagtagagct ttatcaataa tgataatttt taaaaaatag tattttttta ttgaataatt
     9001 tcttagatat tttaaagtat ataaattttg catatatgaa ttggacatta tcaaatgtgt
     9061 tataatatta aaacaacaaa taaaattatt atatttttta gaataataat tttatttgtt
     9121 tactaagttt atgatagggt atttatttaa ggtaataaaa tattatataa aatattttaa
     9181 agccttaggt aatattaggt agaagtttta gtaaaatatg caaaaactaa ttgattatgt
     9241 accttgtatg ggtatataat agatattgtt ttaataaaat atttgaaagg gaatgatttg
     9301 atgaaaatta ctttaccaga aacagctatt gatactttaa aaagtatctt aaaagacaat
     9361 caagataaac ctaataatat aagagtatac tttgcaggtg ttggttgtgg aggtccttct
     9421 tttggattag ctttagatga gaaaaaagaa gatgacttaa cttatgaagt tggagaatta
     9481 caatttgtaa tgagctctga tgaatatagt cagtatggag atataataat agaagataca
     9541 ggatttggat ttagagttat tcctgaaaat atgaaagatc aaggtggagg aggatgctct
     9601 ggttgttcag ggtgccacta agattaagtt tttagtaagt ttagaactct ctttatagaa
     9661 ataaatttct attttaagag agttcttttt attttttggt gagataatgt ataataaaaa
     9721 tgtattttac taatttttta tatgtaagga gaattattgt gagaagaaga cgaaaaaaag
     9781 gtgcagatga aaagctttta agttatacaa aatatgtttt aagagacgat atagacaaat
     9841 taaaaggcaa gtggaattta aagtttagaa atgataatcc aatacatgtt gaatttggga
     9901 ctggaaaagg taagtttata acaactttag ctaagcagaa ccctgacata aattatattg
     9961 ccatggaact taaggaagaa gttcttttaa aagctgttga aaaagcagat gcttctaatt
    10021 tgaataatat actattttta tggggagatg taagcaatat tttggattac tttgaagcta
    10081 aagaattatc gagaatttat ataaatttct gtgacccatg gcctaaaaat agatggagta
    10141 aaagaagact cactcattct ggatttttag aaatgtacaa tagggttttg gaagatgatg
    10201 gcgaaattca ctttaaaaca gataatgaaa aattattcga gtttagttta aatgaaattg
    10261 ctgcaaataa ttggctatta aagaatattt ctcttgattt aggaaatagt gagtatgaaa
    10321 ataatgttac aacagagtat gaagacaaat ttatgtcaca aggtatgcga atatttagat
    10381 gtgaagctaa aaaacgtaac tagtaatatg tttataagtt taatagtaga aatttatttt
    10441 aatttatatt aataaagtgt acgaaagtag gtatatataa caaggaaaca gaaccctctc
    10501 atagaaatta aattattata ataaaaaatt tatgtgggag ggtactatga atttagctaa
    10561 tttaatacca tttattattt ctgcagcagc tttaagtcca ataaattcaa atgataaggt
    10621 tttgagcgaa aatcaactta aagatatttt tcctgatgca aatttaagag cagttgtaaa
    10681 aagatatata aatccagatg agatgactat aagcaatata aaggctttag atggagaatt
    10741 ttatgcaact ggtgagagta tttcaaattt aaaaggtata tcatatcttg aaaatgtaga
    10801 caattttatt ttttggaata ataatataaa agaggttcct aaggaagctt taagtctaaa
    10861 agatatggat tcaataaatc ttgccaataa ttatttgatt gatgacgatg tagtaaattc
    10921 tctttctcat aatggtgtgg atgtaaattg cgatttaaat tttattgata caaaagataa
    10981 tcagtataaa ctggactcta aatatcataa tgttgatata gcaaaaggtg aaagtattga
    11041 tttgagaaaa ataattacga agaaaattga tagttattat aaatactggg aagttactga
    11101 taaccttcca aaagatttaa attttattgt atctgtaagt gataagagtg tactttcatg
    11161 tgaagatatg ataattaaag gtaataaaac aggtaaagca gtggtaaaag ttatactaag
    11221 cgacaaaaat gatttaaata catctcaaga agttctaatt actgtcaatg taaaataatc
    11281 tgtcaactta ttcaatcatt ctattttcaa tttaagtgta taatcaaaat aaaggttgtt
    11341 agtaacagaa cgctaatatt agtgatttgt tactagcaac ctttaaattt tatagaaata
    11401 gattatctaa aaaattccat ttcttctgtt agtagcccat attcctatta aaattatcag
    11461 tgaatatatt atatacaaaa ctattcttat atattggaca tctaatccaa aaaattcaat
    11521 gttactctgt gaggtatgca acatcacaag gattgcagat ataccaataa gcaaaggatt
    11581 gattactatt tgcttatcag ttcttaaaat cattatttta cccatccaaa ttattgataa
    11641 tataaaaaat aaaattttaa gaccaaattc taatgtatct accaaagcga aaacatccct
    11701 tcatttataa taaatttttc agctcaacaa aatataaaac cctacaaaca acttttgaat
    11761 ccataataag ataacttgat taagcactat gaaataagta ctataaaact tcatctacat
    11821 taatatatac tacactatac ttcaaatttc ttaaacataa attaatgaaa ttaacaaaat
    11881 tttattcaga aacatataaa gatatatttt actatttaaa tgcatatcat tatatataca
    11941 gttttaggag gtgaggtata tggaatctaa attttttaat tggtacactc aatccttagg
    12001 gggaactttt ggcattatat catgtatatg cgcatattta aatggataca tgtttgttta
    12061 tacaaatagt gctagcaaat attttgaaag tataggattt ttgggaataa tttcaagtta
    12121 tacactactt ccactttgta tgcttacttt gatttttgga gttatatatt cttatactcc
    12181 agaaacatcc gacaaaagta tttttaatat accttttttt actttaaata aaagattaat
    12241 acttattaca gtagtttttg gttttatagg tgctagagtt tactttataa ttcctgcaat
    12301 tctaataact tttaacctat atagcaatgt tttgatagct aaattaaagt acatcctatg
    12361 taaaaataaa gaaaatgata ctaatttgaa agaaagcgaa gtctaaattg cataatattg
    12421 gtatttgtct taaaaattcc cgaggtatta atttgtattc tcttgtattt tgcaaaaaat
    12481 attgaatttt attcatttta tacttataat ttatattaat aatggaaaat taaaacaaat
    12541 ataagttact ggttgttaat ttaaaattaa aagatatatt gatgtgttga ttctctagtc
    12601 agcacttttt cttttataaa tttgtttttt gtgatataat attagcacca ggtaataata
    12661 caagggggta acaaaatgaa catcaatgaa attaaggaat tattaaaggc catagactca
    12721 actaacttag aatatgtaaa acttgaaagt agcgatttaa gattagaagt atctaaaaaa
    12781 gctcaatcta caagttcacc tgttttaagt gtacaacaag aatcagtagt ggatctttct
    12841 ttagaaaagc cagttgtaaa cgatacacct gtcacatcta atgagaatct atcagtagta
    12901 gtagctcctt tgatgggaac attctatgat tctccaagtc cagatgcaga tagctttgtt
    12961 aaagttggcg atgtagttga agaaggcgac actttatgta tattagaagc aatgaagctt
    13021 atgaatgaga taacaagtga aattaaaggt gaaataattg aagtattagt tagtaatgaa
    13081 gaactagtag aatataatca acctttattt aagataaaac cattgtagta ggaggtattt
    13141 aagttgataa aaaaaatact agttgcaaat agaggcgata tagcagttag aatcatcaga
    13201 acatgtaaag aactaggtat aaaaactgtt gctatctatt ctgaaataga taaagattgt
    13261 tttcatagat atatagctga tgaatctatt tgtataggac caaataatat aagtaagagt
    13321 tataataata tagaaaatat aatctatctt gctcttaaat taaagtgtga tgctattcat
    13381 ccaggctttg gatttttatc agagaatcct gaatttgcaa agcaatgtga agataataat
    13441 attattttta taggtccaac aagagaacaa atgattttga tgggagataa atctagagca
    13501 agagaaacta tgatggaatt gaatatccct gttgttcctg gttcagaatc tgttttgaaa
    13561 actaaggaag aagctttaga agttgcaaga gaaattggat atcctgttat gataaaagcc
    13621 tcaagtggag gcggtggaaa aggtatgaga atcgttagaa aagaagaaga acttttttca
    13681 aattttgata tggcaagttc agaagctcta gcagcatttt ctaatagtga tttatatatg
    13741 gaaaagttta tagaaaatcc tagacatata gaagtacaag tatttggaga taaacattca
    13801 aatgcaatac atttaggtga tagagattgt tctatgcaaa ggcgaaatca aaaagtaata
    13861 gaagaatctt taagtccata cctttcagat gaagaaagac aaaaacttca taaaattgca
    13921 gtagatatag taaaaggagt aggttatatc ggtgcaggta ctattgagtt tatagttgac
    13981 aaagataaaa acttttattt tatagagatg aatacaagaa tacaagttga acatccagtt
    14041 acagagatgg taacaaatct tgaccttata aaacttcaaa tatctatagc taatggggat
    14101 aaaattcctt ttaaacaaga agatattact tttagaggtc atgctattga atgtagaata
    14161 aatgcagaag attcatcaaa gaattttgct ccatcaccag gtaagataga atcattaaat
    14221 ttacctggag gttttggtgt tagatttgat acttttgtat atgcaggata tactatacca
    14281 cctttatatg attctatgat aggtaagtta atatgttggg ctgaaactag agaagaatgt
    14341 ataagtagaa tatatagagc tctagacgaa attattgtag aaggtattaa tacaaatgta
    14401 gaatttcaaa aggcacttgt aacaagtgag gagtttagag aagatactca tcataccaaa
    14461 tttatagaag atgtctttat gaagaaagaa tttgctacat tataaggagt aaatagatta
    14521 tgataaaaaa atttttatca tcaaaagaat ctaagtatgt tacgatttct ttagatgaca
    14581 actttaaaaa gaatagtgta gatgataaat tttggaccta ttgcaaaggt tgtgatagtc
    14641 atgtatttag aaaggacata gaagaaaatt catttgtatg tccaaaatgt tcaagacatt
    14701 atggcttaag agcaagaaaa agaataaatt tattgattga taaaggaact tttatggagt
    14761 ttaattctga tgtagaattt caaaatccac taaattttcc taaatacaaa gaaaaagtag
    14821 actcttataa ggaaaaaaca aaagagtcag aagctgttgt cacaggttat ggaagaataa
    14881 atggaattaa aacagttatt tgtgtaatga atcctgattt tatgatggga agtatgggtt
    14941 ctattgttgg ggaaaaaata acttattcaa tagagtatgc tgctgaaaat aatttaccta
    15001 taattatttg ttctgcttca ggaggagcta gaatgcaaga aggtatggta tctcttatgc
    15061 agatggcaaa gacttctcaa gcactttcta agcttgaaga aaaatcattg ccatatatat
    15121 cagttctaac tgaccctact actggaggag taactgctag ttttgctatg cttggtgata
    15181 ttataatttc tgagccaaat acgttgatag gttttgcagg tccaagagta atagagcaaa
    15241 caataaatca aaaacttcct gaaggatttc aaacttctga gtttttactt gaaaaaggtt
    15301 ttattgacat gatagtagat agaagaaaaa tgaaggaagt attatatcaa attcttgcta
    15361 tgcacaagaa atagggcata aaggaggaac tgttttgatt gaaaataata gtgaaaaaat
    15421 aaaagtctta gaaaatgata ttaaacaatt aataactatt tctaaggcaa ataatataga
    15481 tttaagtgat aaaataaatt cacttaatga gaaattggaa aagcttaagg aagatgcttt
    15541 ttcacatcta tcagcatatg aaaaggtaac actaagtaga gatataaaga gacctacgac
    15601 attggaatac atagaacata tttgttctaa ttttttagaa cttcatggag ataggctttt
    15661 taaagatgac ccttctatgg ttggaggaat tggtcagata ggaaagttta atgttactat
    15721 agttggacac caaaaaggaa gagatacaaa agaaaatata aaaagaaatt ttggaatgcc
    15781 tcatccagaa ggatacagaa aagcattaag acttatgaaa caagctgaaa aatttaatag
    15841 acctataata acgtttattg atacatcagg agctttttgt ggtctagaag cagaagaaag
    15901 aggacaagga gaagctattg caagaaactt acttgaaatg agtaaacttt ctgttccagt
    15961 aatcactttt gtaattggtg aaggtggtag tggaggagct ttaggtatcg gagtaggaaa
    16021 tgatgtttgt atgttagaac attcagttta ttctgtaatt tctccagaag ggctttctag
    16081 tattctattt aaagattcat ctaaagctaa agaagcttgt gatgttatga agcttacaag
    16141 taatgattta tatgatttaa aaataataga taagattata aaagaacctc ttggaggagc
    16201 acaaaaagat gtagaagctg tttcaaaaga aataaaagct tatattttag aaagattaaa
    16261 tcattacaaa gatatggata aagaggaaat tatagctcaa agatataaaa aatttagaaa
    16321 tattggaaaa tgcttataga atataaagaa atttgtagtt ttttcataat aaaatggtta
    16381 aaaaaaataa aggttttctt aaaacgatta tagaagtata tttttataaa agtatacttt
    16441 aaatgatgat tgtaattagt aaatggcaat tatgatttag tactttaggt aatggatact
    16501 ttaacaaatt aacaaggaga tgttgaaatg gaggtactaa aagtttcatc aaaatctaat
    16561 cctaattctg tagctggagc cttggctgga gtactaagag aacgaggcgt tgcagaaata
    16621 caagctatag gagctggagc tctaaatcaa gcgataaaat caattgcaat agcaagaggt
    16681 tttgttgcac ctagtggaat ggatttagtt tgcattcccg catttactga tatagaaatc
    16741 gaaggtgata agaaaacagc cattaaactt ataattgaac ctagataata tatctcttta
    16801 ttagaattta attaagtttt aaaggttggc tatagaaata ggacactata gtgaaccttt
    16861 tttactttat atgttttatt ttatgtaact tttaatgtat aatcttaagt aaacaaataa
    16921 gattggagga aatatacatt atgaaattaa aattattggt agaagagtat gcagtatgta
    16981 gattaaataa tgacagtaaa ataccaacat ggatagatac agagaagttt tactctataa
    17041 ctagaactga tgatgaatta agtgttgtat gtttaaataa taatattcct tctgatgtta
    17101 agtcagaaaa ggagtggaga atacttaaga tactaggtcc tttagacttt tcacttgtag
    17161 gaattttatc taagttaagt agtttgttag cagacaacaa aataagcata tttgcaattt
    17221 ctacttatga tacagactat atcttagtaa aagaaaaaga tatagaaaat gcttgtaaaa
    17281 ttttaagctg taatggatat gaagtagagt agttaaatac ttttacatga ggacttaact
    17341 tatgatttat ataatcgaaa ttttaaaaat aagtgtaata aatatttttt gtttcatagg
    17401 aatatttatt gtatttggat tgatttttag cattacagaa aatctaaata ataaatttat
    17461 attttcatct tttggaaaaa ctggaattat tgtaactgga gctataggaa cgtttgttca
    17521 tgaattgagt catttgataa tgtgtttaat tttcatgcat aaaataaatt cagttaaatt
    17581 tttcagacca atagaaagta aaaatgatgg tatactagga tatgtaagtc atagctataa
    17641 aaaagataat ctttatcaaa gtataggaaa tttctttata ggaattgcac ctattatagg
    17701 tggaacaata atgattattt taatttttaa gatattactg ccagatagtt atatgaaagt
    17761 gactcaaaat atagatttaa agttatatgt aagtatgatt aataatttaa atataaatgg
    17821 ttttatacac attattttag gtgatatttc caattttata agaataatgt tgttaacacc
    17881 tagtgtatat tcattgaagt attttatatt tatgttttta atgtattcta tatctacaca
    17941 tatgtcactt agtagtgctg atttaaaaaa tagcttaaat ggtctgagct ttatagttat
    18001 agttattttt gtaataagtt caattacata cttatttgga tttgataact tgaatatatc
    18061 cagtttaata gttaagtata acatatttat aagtttcttt atggcaatag ggtt
//
LOCUS       DAGSWI010000082        17957 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.082, whole genome shotgun sequence.
ACCESSION   DAGSWI010000082 DAGSWI010000000
VERSION     DAGSWI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.082"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     regulatory      57..159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            253..1629
                     /locus_tag="MJF80_002500"
     CDS             253..1629
                     /locus_tag="MJF80_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBZ0281154.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1726..2871)
                     /locus_tag="MJF80_002501"
     CDS             complement(1726..2871)
                     /locus_tag="MJF80_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBZ0281155.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDINSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3124..3831
                     /locus_tag="MJF80_002502"
     CDS             3124..3831
                     /locus_tag="MJF80_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281156.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3878..5473
                     /locus_tag="MJF80_002503"
     CDS             3878..5473
                     /locus_tag="MJF80_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281157.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIGSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVKIFKNL"
     gene            5576..6658
                     /locus_tag="MJF80_002504"
     CDS             5576..6658
                     /locus_tag="MJF80_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBZ0281158.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKEDKVNSNLDKVIEKLN"
     gene            6807..6968
                     /locus_tag="MJF80_002505"
     CDS             6807..6968
                     /locus_tag="MJF80_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBZ0281159.1"
                     /translation="MKQDKKNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGDRI
                     DKKKMNRPS"
     gene            7309..8577
                     /locus_tag="MJF80_002506"
     CDS             7309..8577
                     /locus_tag="MJF80_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBZ0281160.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            8772..9560
                     /locus_tag="MJF80_002507"
     CDS             8772..9560
                     /locus_tag="MJF80_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBZ0281161.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSIRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            9560..10009
                     /locus_tag="MJF80_002508"
     CDS             9560..10009
                     /locus_tag="MJF80_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBZ0281162.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            10013..12277
                     /locus_tag="MJF80_002509"
     CDS             10013..12277
                     /locus_tag="MJF80_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBZ0281163.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            12417..12728
                     /locus_tag="MJF80_002510"
     CDS             12417..12728
                     /locus_tag="MJF80_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281164.1"
                     /translation="MAGNLNNMRAVNNFRGDKSILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLSTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
     gene            13014..13514
                     /locus_tag="MJF80_002511"
     CDS             13014..13514
                     /locus_tag="MJF80_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBZ0281165.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            13571..14557
                     /locus_tag="MJF80_002512"
     CDS             13571..14557
                     /locus_tag="MJF80_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBZ0281166.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            14607..15329
                     /locus_tag="MJF80_002513"
     CDS             14607..15329
                     /locus_tag="MJF80_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBZ0281167.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            15358..17292
                     /locus_tag="MJF80_002514"
     CDS             15358..17292
                     /locus_tag="MJF80_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBZ0281168.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMLIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            17285..>17957
                     /locus_tag="MJF80_002515"
     CDS             17285..>17957
                     /locus_tag="MJF80_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281169.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKHLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDR"
BASE COUNT         6829 a         1782 c         3092 g         6254 t
ORIGIN      
        1 tacgtatata ttgacaaaat taattatata aattaaaata atattcgtaa agaaaaattc
       61 atgttttgta ctgtataaca tataattttg acaatatggg tcataagttt ctaccggaat
      121 accgtaaata ttctgactat gtatactatt atttagttaa ttaaatattt aattaactga
      181 attatgaagg atacggctca gcttatcaac tactgtgtcg tttttttgta aaatttaata
      241 ggaggtgttg ttatgcaaac aacaaacagt agtactatgc agaaactttt tccaattttg
      301 actaataaaa atgtcgacat gaagaaggaa ataattgcag gagtaactac ttttttaact
      361 atggcttata taatagctgt aaatccaaat attttatctg agacaggcat gcctgcaggt
      421 gcacttgtta caggtacttg tttagcagca gcatttggat gtattttaat gggtgtagtt
      481 gctaaccttc cttttgcttt agcatcaggt atgggtctta atgcattttt tgcatataca
      541 gtagttttac aaatgggtgt tccttgggaa gtagctctta cagcagtatt tgtagaaggt
      601 attattttta tagtattatc agtaagtgga gttcgtgagg ctgttgtaaa tgcgatacca
      661 aaaaatatga aattagctgt tacaggtggt ataggaatat ttattgcact tataggtcta
      721 gttaacagtg gaattgttat tggagaccaa gccactctta tcaaaatggg tagatttaca
      781 ccagcagtaa taataacttg tgtaggatta ataattattg ctgtattaga taaaaaaaga
      841 gttaaaggtt ctatactttt tggaatagta gtgagttcat tacttgcttg ggcatttgct
      901 tttataaatc cagagtatgc acaaaaatta ggtatatatt tacctggtgg attatttaaa
      961 ttcgaaagtt tagcaccaat agcaggtaaa atagatttag gatatgtact acatccaaca
     1021 aatataggtg gttttttagt agtagtttgt acatttttat ttgttgattt ttttgataca
     1081 gtaggtacat tagttggtgt ttgttctaaa gcaaacatgt tagatgaaaa agggaatgtt
     1141 cctaatgtag gtagagcctt acttacagat gcggttgcaa ctacagttgg tgcaggactt
     1201 ggagtatcta cagttacaac ttatgttgag agttcaacag gagttatcgc aggtggaaga
     1261 actggatgga cagcaataac agttggaatt ttattcttag ctgcaatgtt tttctctcct
     1321 gtatttatag caattccatc atgtgctaca gcaccagctt taatatatgt tggatattta
     1381 atgcttggta cagttaagga tatagagttt gataatataa ctgaaggtgt tccagctttt
     1441 gttactattg cttgtatggc attaacatat agtataggtg atggtttaac tttaggtata
     1501 ttaacttatg ttttcgtaaa tatattttat aatatatttg gagctaaaaa ggcagaagac
     1561 aaaaaacatg tttctgtagt aatgattgtt ctggcaatat tatttataat aaaactttgg
     1621 ctactataat aggtattatt taaaatttta atttaatata aaataaaggg ctgtaattta
     1681 ttatcaattg tgatgataaa ctacagctct tttgtatttg ttgtattatt taataatgtc
     1741 tattagttct ccattttgta aagttttagg tgtaattact atattgaact ctttttgcaa
     1801 atcttttata atatctaatt ctttttttgt tattattgag attgtgttac cacttcgatt
     1861 tcctctagca gttcgaccac atctatgcag gtattcattt ttactttttg gaaaatccaa
     1921 gttaaaaaca tgacttacat ccacaatatc tagtccacga gcagataaat cagttgtaat
     1981 taaaatcttt gctttaccaa gtttaaattt atttattgca ttctttctat cttctttttc
     2041 catatttcca aaaattccta ttgctttgta attatgataa tttagttttg atactaggac
     2101 ttctatattt ttttcattat ttacaaatac aatagctttt tttggatttg tagctgctaa
     2161 tgcctttcta agaaaagtaa atttatccct tatctcacct aataaatatg agtgattgat
     2221 gttaggattt atttgtgatt tttcctttgt ttttattatt tcaaactcct tcataagttt
     2281 atcacaaatt tttatagtgc tatcagtaag acttgcagag catcctataa tttgtctatc
     2341 tcttaacgtt gttcttatta tgtcttctat acaggttata ttttttccat tcaatagatt
     2401 gtccacttca tctaagacaa tagtttttat attatgtgat tttagtttct tttgttttat
     2461 caagtctaaa actcttccac aagagccaat tactatgtgt ggttttactg cttttatatt
     2521 ttttatttgt ttttgtatat tgacctcccc aattaaagct aaagaagtaa cggagagttc
     2581 tgcattttta gccaataatt caacctggtt tgtaatctgc attactaatt catgagtagg
     2641 tgcaagtata agagcttgtg tttctctttt tgatgtatca attttttcaa atataggtaa
     2701 aaggtacgct agtgtttttc ctgttccagt ctgtgaatta atcaataaat ccttattttg
     2761 aattatattt cctataacaa gtgactgaac ttctgttgga gaatttatgt cttgtttttt
     2821 gagtccatct attagagtgg aacttatttt tagttgttca aatgtattca tattttgctc
     2881 cttatatttt aataattata gtcaactatt ttttattata cattgtttat tcaaatcgaa
     2941 ctatatattc ttttataatt atgtgtaatt tgaaagtaaa tatcaattat atttatacct
     3001 ttatatttct gatacctttg cattttatag atttctaaaa taaacaaata aaagttgtaa
     3061 ttgtttgata tttgttatat attatatata acaaatatca aaactataat atggagggaa
     3121 attatgttaa aagtaatcaa tgtatctaaa tcatataaaa ataaaaaagt agtaagtagt
     3181 gtctcatttg atgtaaatga tggagagctt tttgggttta taggacataa tggagcaggt
     3241 aaaactacta ctattaaagc aattgttgga atacacgatt ttgaagaggg agaggtacta
     3301 ataaattcta aatctataaa aaaacagcct gttgaatgta aaaaggaaat ggcatatata
     3361 cctgataatc cagatttata cgagtcatta actggtatac aataccttaa ttttatagct
     3421 gacatatttg aagttgccaa agataaaaga gaagagttaa taaaatatta ttcaaataag
     3481 tttgaactca ataaagcatt aggagattta atatcttcat attcgcatgg tatgaaacaa
     3541 aaattagcta taatttcggc tttgatacac agcccaaaaa tcttaattct tgatgaacct
     3601 tttgtaggtc ttgacccaaa agcttccttt atattgaagg aaattatgag ggagttttgt
     3661 gacaaaggtg gttgtatatt tttctcaaca cacgttttag aagttgctga aaaaatttgt
     3721 gataagattg ccataataaa agatggaaat ataattgctt atggaactac agaagaagtt
     3781 aagggaagta attcacttga aagtattttc atggaattaa tagagaaata aaatgttttt
     3841 tatacaatat agtgtctaaa gtgaggaatg ataaaatatg agcaatctaa gtatattaat
     3901 aaaaactaat ataataaatg aatttaaatt aaatgcgtta aaaaaagctg atggtagaga
     3961 aaaacaaaaa ataatgggaa tgatgctttc agttgttata atagcgggtc tattaatttt
     4021 ttatgtaacg agcttaagtc tctctttagt tgatatctta aagcaaataa atcaactgga
     4081 aatgctactg atatttggtt ttggattatc aacattaaca actattgtta catcattata
     4141 taaaacgtca tcatatctat ttcaagcaaa agatttagac cttttgacta gtttacctat
     4201 aaaagaatct actgtactag caagtaagat attaatgttg gttggagcaa actatttatt
     4261 ttcttcaata tgtatgatta tacctgcagt ggtttacttt ttaaattctg atgtaagtat
     4321 aacttatttc ccttatttga tattgctatt tttagcttta ccattattac caatagttgc
     4381 ttcatcaata atttttctat ttatagggca gatatcatca agaataaaat acaaaaattt
     4441 agtattaatc ataggtagca ttatattgat gttgctttat accctactta tgtcaagact
     4501 taattctatg tcagttgaaa tgctaaagaa tagtgtgtct atttcagaga caattagaaa
     4561 agtatatcct cctatatatt attttgttga tgctcttaaa acaggaagta tagtttcatt
     4621 tttagcattt atagccatct caataatacc attttttatg tttgtaactc tgtttgcaaa
     4681 aaaatttaaa gatataaatg ctaagatgag tgaaggatat aagtcaaaaa attatgaatt
     4741 taagggtcaa aaaacttcaa gaccttcaaa agctttatta aaaaaagaat ttaagagata
     4801 tacttcttcg tttatatact ttttaaattc atctgcaggt ttgttgtttc taatagtagg
     4861 tacaatttta atattattat ttggagcaga taaagtgggt gagttattga aattgaatat
     4921 agattttaat cttataaaaa tacaattatt gggagtattc ttctttataa tagtaatgaa
     4981 ttgtacaaca tactgttcaa tttctcttga aggaaaaaac ttatggatac tgaaatcttc
     5041 tccaatagat gaaagagaaa tattctggtc aaaaatattg atgaattttt tattgaatgc
     5101 accactatct attttatgtc taatattagt atctataaga ttagactttg aaattcagtt
     5161 tattattgta tcaatctgtt tgattggaag tttttcaatg tttgtagcat tgatgggatt
     5221 attatcaaat ttattatacc caaaccttga ttggaaaaat gaggtggctg tagtaaaaag
     5281 aagtgcaagt atgatagtta caatgttagt atcattagct tatgtaatta tacttggagg
     5341 tatatatgta ttacttggaa tttcgttttt aaatatatat attattttgg caagtatact
     5401 tactttgata ttagatttta tgatatggag aattatttct actaaaggag taaaaatatt
     5461 taaaaattta taagtgtatt tatgtcaaat aaagacttat cttttttagt cttgcaaata
     5521 ataggttaag tagtatacta tgtatagtga tatgaaaaaa tttaggagaa aataaatgag
     5581 aaaattatcc aaaagagaaa aagattttat tcaatatgga tttttattat ttttgatatg
     5641 tattactact tatttagtat taactacttt agatataaaa ctaataccta aaattataag
     5701 gctagtaaat aagacttata tatttcttgg catattgaca attatagtat atatgataat
     5761 tgaatctatt gtaactcatt tgataataaa tgcagttcaa aaaacaaaag taaaatttat
     5821 tggtattaaa atggctacaa tgggatttta ctataactta gttactccat ttgcatctgg
     5881 gagtcaacca atgcaaatat atgcacttac aaaatatgat gtaagcctta gtaaatcagt
     5941 ggctatagtt actaataaaa ctgtagtatt tcaaagtaca gttactgtat tttgtggaat
     6001 tttaattttg ctaaataagt cattactgat ggagcaagta cattctgtga aagtcttgat
     6061 aacttttgga atgattatga atgtaagtat gttgttaggt ggactactaa tagtatttag
     6121 tccaaaacaa gtaaaaatga tagcagaagt tttgattaat tttttatcta aatttaaaac
     6181 atttaaattt ttagattcaa aacgtgatag aataaatcat tatattgatg attataatta
     6241 ttctataaaa atatttatta aaaatgtaag aatcttagtt ttaagtatat tacttactgt
     6301 gttacaatta atcgcatatt tcagtatagt ttattgtgtg tataaagcct caaatttaag
     6361 aggagtttca tatgtacata taatgacatt acaggtcttt ttatatatgg ctatatcacc
     6421 aattccaaca ccaggaaatg ttggagctaa tgaggttaca ttttttacta tgttctccaa
     6481 tataattcca aaagaattat tggggtactc ggtactctta tatagtgggt ttgtatacta
     6541 ttttatatta atagtttcag gtttatttac atttggaacg cattatacat taaattcttg
     6601 gaaaaaggaa gataaagtaa attctaattt agataaggtt attgaaaaat taaattaata
     6661 aaataattag ggaaattcta tatataaata tggtgttgag taagctatat tatattgaga
     6721 tatttccctt ttttgattaa aattatagaa atttgaaata ataagtaata aacagaaaga
     6781 gtgatattgt tttgaaagga gaaactatga aacaagataa aaaaaatgaa aaacaaagtg
     6841 agatttctaa gataaaacaa agcaatgata ataaacttga aatagctcaa gaattacatg
     6901 taagtaaaaa tatagggaat atgcttggag atagaataga taagaaaaaa atgaatagac
     6961 caagttaatg aaaatgttga aaatggtaca ttttaaaaaa tatatataat gaaatgtttt
     7021 attgttaaat ttaaacttat tttaatggag gaaaaagtcg aaaaatatct aaaaagatat
     7081 ggatataaaa aatattaata tatatatact ataaacttga caaaatcaac cttttaataa
     7141 ctagcaaaaa atagcaaaat tatataagcg ttatttaatt gtcaaaaatg aaagctaaat
     7201 tgcatgtcct aaaaaaaatg attgataaca tttatttgtg tgctataatt atatagtaaa
     7261 acaataagta tattgcataa tgtatgcaaa tcgggaggag agacggacat ggaagcatct
     7321 atacacacaa ttgccagcaa taatttattg ataatttttg ctattgtaag tataactgga
     7381 ataatttgta gtaagcttag tgaaataata aaggttccag atgtagtgtt atatttactt
     7441 gtaggtattt taataggacc atcattttta aaatttatag atattcgtgg atttcaaata
     7501 gaaaatcaac taatacttac atttggttct gcatttattt tatatttagg tggaaaagaa
     7561 ataagtttaa aagtattgag aaatgtgaag atatctgtat tcttattagc tactttagga
     7621 gttgtgatat ctgcttttat aatgcaacaa gtaataggat ttacttttgg gataagtgca
     7681 atgactgctt tactagcagg aactataata gcatcaacag acccagccac tcttgtacct
     7741 atatttaatc aggtaaagat aaaggatagg gtaaaacaaa ctgtaataag tgaatcagct
     7801 tttaatgatg caacaggagc tatactaaca tcagcagtgt tggctgtaat tttgtcaaat
     7861 aaattttcat taggtgaaaa tatttatgaa ctggggacaa tggttattgt cggagtttta
     7921 gttggtttaa ttacaggaat actgcttttg aaattagtta atgataaacc ttatggaata
     7981 tttaaagatt ttgcaccaat aatatctgtt atttcagttg tagttgcata tgaattatct
     8041 acaaaattag gtggaagtgg atatatgtct tgctttattg taggtataat aactggtaac
     8101 aagaaaaact ttaaaatttg gttaagtcaa aaatcttatg atgcagattt ttatgttgct
     8161 gaaactttag gtactatttg tcgtatggca atatttataa tattgggtag ccaagttaat
     8221 ttggctcagt taagtaaata ttttatgcca tctgtaatta cagtattagt attaatgttt
     8281 atagcaagac cactttgcgt tttagtttgt acacttgtag acagggaagc aaaatggagt
     8341 aaaagtgaaa tattatttat gatgtgggta agggaaacag gcgtaatacc agcagcactt
     8401 tgtggtatta tttcagcaat gaaagttcct ggatacgaag tgatttcatc tgtagtattc
     8461 atgactatac taattacatt agtaatacaa ggaagtacta caaaattagt agctaaaaaa
     8521 cttggattat tagaagaaga agtgataaat ataagtgaaa aagtatcaac tttttaaagt
     8581 tttgctttaa aatagtacct gttaatggct attttaatat tgtctaatga tgattattga
     8641 aatagtcatt tttttatgta taagtaattt acagaattta catattagac atatttatta
     8701 tcaaaatttg ttgaagctat gatataatta gttgtattta aaataagaat agcaaataag
     8761 ggaggaaatt tatgtttacg gttatggata ttgtacagcc agatacagta gaagaagcgt
     8821 actcaatttt aaataaaaga aaaactaacc aagtaattgg tggaagtgct tttttaagaa
     8881 tggggaaaaa aagaatagga acaggaatag aattatctaa attaaattta gattatataa
     8941 aagagtatga agattacgtt gagattggtt ctatgactac ttttagaaca ttagaaacaa
     9001 gttccataat aaaaaataat tttggaagaa taatagaaga ttcagttaaa aatataatag
     9061 gagttcagtt tagaaatgta gtaacagttg gtgcaacagt attttctaaa tatggttttt
     9121 ctgacttgat agtagcatta ctttcattag atacggaagt agaattatat aatattggga
     9181 gaattagtct tgaagagttt ctaaatagag attatgagaa agatttactt attaagatat
     9241 acataaagaa gacaaataag aatgcttctt ataaatcaat tagaaatgca aagagtgatt
     9301 atccaattct aaatgtatct gtatcaaaac atatggaaca atttaagtta tgtgtaggag
     9361 caagaccgca gaaagctacg attgcaaaac aagctagtga atttctatct aacaatgaaa
     9421 tcaatgaaat taatatagat aaagcagtag aaatagcatc agaagaacta acttttggtt
     9481 ctaacatgag agcatctagg gagtatagaa aagctatgag taaagtactt ttaaaaagag
     9541 ctataatgga ggttatttaa tgttagtaaa gattaatata aatgggaaga aaaaaagtgt
     9601 tgatatagaa ccagaagaat atctggttga tactttaaga aaagttggta atttaagtgt
     9661 gaaaagaggt tgtgacacag gttgttgtgg tttatgtaca gttttaattg acaagaaacc
     9721 tacattatct tgtgcaacac tgacattgag agctttagat aaagaaataa ctacaattga
     9781 aggtttagaa aatgaagtta aagaatttag tgaagttttg gtaaaagaag gagctgagca
     9841 atgtggtttc tgttctcctg gctttatact tactgtactt gcaatgaagg atgagcttga
     9901 aaatcctaca gatgatgaaa taaagcatta tcttacagga aatctatgta gatgtacagg
     9961 gtatatggga cagttaagag ctatcaaaac ttatctggag gtaaaataga acatgaatat
    10021 agttggaaaa ggaatcaaga aaatagatgg aatgagtatc acaacaggaa agcctgtata
    10081 tacagatgat ttatcagcaa aagatgcttt ggtggtaaag atacttagaa gtcctcacgc
    10141 ttatgcaaaa ataaaagata tagatacatc aaaggcagaa atggttgatg gtgttgaatg
    10201 tgttttaaca tataaagatg taccaaaatc tcgatttaca cttgctggtc aaacatatcc
    10261 agagccatct ccatatgata gattaattct tgaagatgtt gtaagatatg taggtgacga
    10321 agtagctata gtggcagcta tagatgaaaa aactgcaatt aaagcaatga atcttatcaa
    10381 agtaaagtat gaagtactta cacctgtttt ggattttgaa gaggcaatag gaaataaaac
    10441 tatggtacat gatgaagata gacattgtaa ctttgatata ggtatgcaaa gagaaaaaaa
    10501 tatagtatcc catcatgatt atacaaaagg tgatgtagaa aaagtattaa gtaattgtga
    10561 tgtagtgatt gaagaaactt attatactca agctcaagca catgcaatga tggagacgta
    10621 tagggcatac aattatttag accatatggg aagattggtt gtagtaagtt caactcaaat
    10681 accatttcat gtaaaaagac atttatctcg tgcactagaa ataccagcta gcaaaattag
    10741 ggttataaaa cctagaattg gtggcggatt tggtggtaaa caaactgcat gtgttgagat
    10801 tttttctgca attgttactt taaaaacagg gaaaccagct aaaataattt atgatagaaa
    10861 agaaactttt aattgttcaa caagtagaca tgcaatgaga ttaaaagtta gactaggtgc
    10921 tacaaatgat ggcataataa aggcaataga tataaaagca ctttctgata caggagcata
    10981 tggagagcat gcttctacaa ctattggttt ggttggagaa aaaactttac caatgtataa
    11041 taaagttgaa gcaacaagat ttattgcaga tgtggtatat actaataaga tgccagcagg
    11101 agcacttaga gggtatggag catcacaagg atgttttgct gttgaatcaa ctataaatat
    11161 gcttgctaat aaattaaaaa tggacccaac agatttaagg ttaaaaaatg tggtaacaga
    11221 aggtgaaact acatttgcat atggtaaaac tttaaatagt gtcgatttaa taaaatgtat
    11281 aaataaaggc aaagaactta ttggatggaa tgaaaaatat ccttgtaaac atcttgataa
    11341 tggaaaaatt agaagtgtgg gtatggcaat tactatgcaa ggttctggta tacaaggtct
    11401 tgataagtct tcagctgaaa taaaattgaa tgactgtgga gattatactc ttatgatagg
    11461 ttctactgat atgggtacag gaagtgatac gattttagct caaatggctg ctgaggttct
    11521 tgatacaagc attgaaaata taacagttat atcagctgat actgacttag ttccatatga
    11581 cccaggttca tatgcttcaa gtactactta tgtaacaggt atggcagtag ttaaggcagc
    11641 tcaagactta agagaaaaaa taatttctgc tggagcaact aaactaggtg ttgagaagga
    11701 tgaagtagaa tttgatggta taaaattaac tagtaaaaaa ggtagcactt tattattcga
    11761 tatagcagtt gattctacaa ttggtccaga tactaatcaa ttaataggtc ttgcttctca
    11821 tggtagccca gtatctcctc caccttttat agcaggtttt gcagaaactg aaattgacac
    11881 agaaactgga aaagttgatt tagtaaatta tgtagctgta gtagattgtg gaacggttat
    11941 aaataaaaat ctagctaaga tacaagttga aggtggaatt gttcaaggga ttggaatggc
    12001 attatttgaa gaagttagat acacagaaaa aggaagcatg gacacaaata catttatgca
    12061 atacaagatt ccagctagat gtgatattgg aaatgttaaa gtagattttg ttgaaactta
    12121 tgaaccaact ggaccatttg gtgctaaatc tgttggagaa gttgttataa atacaccttt
    12181 accagcaata caagaagctg tgtttaatgg aattggtgta agaataaata cactccctat
    12241 tacacctgaa aaagttttta tggaaatgaa taaataattg tttatgaaaa tttagactag
    12301 ttttacatgt taaacaacca gttatgtcat acttgttgaa agatatttaa taagtagtta
    12361 ttataatatt atgaatttta cttataaaat acaaaattaa ataaggggat gatgttttgg
    12421 ctggtaatct aaataatatg agagcagtaa ataattttag aggagataag agcatcttag
    12481 aatgtttagt cagctttgag ggtcgttcaa taagtcagag aaaagtaagg gtatttttta
    12541 aagaaaaaca aaatcaaata gaaattgatt ttgcagaaga ggaaatttct aaattggttg
    12601 aaaatgttgt tttaagtaca tcatatcaag aaatgttata tgatgaaata gagaaacaac
    12661 tggaaattga ttgtataggt acttggatga tattatctaa attaaaagat ggtagtagag
    12721 ttcactgatt ttaaaataaa ttattagagc ctaattttaa ggctcttttt tgctgttttt
    12781 aattaacaat agaaaattta tctttagttt gtatataatt ttaatatcat atattctata
    12841 tatataaaat tgaatttgta tgtattatat tgtataatat aaaatgaatt gggaaaatag
    12901 ttcgctaaaa taatttgttt ttataaaatt actgtttaag tttatagatt ttgtataaaa
    12961 taaagttatg ttattaattt ttttaaaaat aaaactttta ctaggagaca gatatgagaa
    13021 aaaatagtat taatatagca ctaactgcag caattacagc aatctatgca gttttaacat
    13081 tatcattggg atttataagt tatggaccaa tacaatttag ggtagcagag attatgatgc
    13141 ttttggcatt tttagataaa ggatatatag taggtcttac attaggttgt tttttagcaa
    13201 atgtaatagg accatatggt gtacctgata taatttttgg aacattagcc acatttataa
    13261 gtgcaagtat gatttatatt actagaaagt taggtaaaca gaataaaagt actttagtta
    13321 tagcttcttt atggccaact gttataaatt cgattgtggt tggtctaatg ttaaatgtat
    13381 tctttgggct tccattaatt ctatctatga tacaggtagg ttttggagaa tttgtagtag
    13441 taacaattgt aggagtacca tttttctgtt ttttaatgaa aaaatataaa aatgtcatta
    13501 atattaaatt ttaaatagtg tatagtaatt taattaattg agagtattat tagtgaaaag
    13561 aggtgtttat ttgtcgaagg taaaaagatt gactgatgtt tggatatata ttaatgataa
    13621 gcaaaagttt acaataaaag agttgtcaga ggaatttaat ctttcagcca aaacaataca
    13681 acgatattta aaagaattaa ataaaatggg acttccaatt caagcagaac aaggtagaaa
    13741 tggaggttat agggtgctaa ataatagcta tatacctcct gttatattta cagaaaaaga
    13801 agttatgatc atactatttg cacttaagtc acttcagatt tataattata agttaatgga
    13861 aatagaaatt aattctataa tgagaaaagt aagttatgaa cgtaaaagca gtataaaagt
    13921 aggtatagaa aatattaaaa gatacattgg ctttgtagct aatgatacta atgatggaga
    13981 ttttaagagt agtaatgtaa ctgagttttt tagagcttct tcagaatctc taattttaaa
    14041 tattaagtat aagtgtggta atcagatttt ggaaaaaaac gtatgcccta taggtatata
    14101 tttaaataag ggagcatggt tttcaccagc atatgattgt aaaagtgata aaataatatt
    14161 agttcatata gataatataa ttgatataaa aagaacagga gaaagtaaaa aaatacatat
    14221 aaacttggaa gaatggttac atgaagtata taacaattta tatggaaata gttcaactac
    14281 taaaaaattt ggtgaaatta tttatctaaa tgtattatta actaaagaag gtgtttctaa
    14341 aataaaggac agttcttgga atttagatat ggagctaaat gaagatggta gtggaataat
    14401 tagtacattc ataaaggttg aagatataga ttgttatgct tcgattttat tttttattgg
    14461 aaataatgct aaggttatag agccaaaatt tttgaataag ctactttatg ataaggccaa
    14521 tgagttaaga tatttttatg aagaaaatat ggtataatat ttagaaatgt tgaaattaaa
    14581 taattattaa ggagatatac gttataatgg aaaattttaa taagaatgct aaaataattg
    14641 gtattggagc tgaaggtatt aatataataa atgaagtaga agaaaaaata aaagcaaata
    14701 tggatataga aaaaataaac ataaatcaag agattgaaaa agaatatgtt agaagtcttt
    14761 tagatggtgt agatatatta tttttgatat attcatctga agataaacat attagagata
    14821 ttataaaagc tgtttcatat atgtcaaacg aaagaagagt tctatctata ggaatggatt
    14881 gttcagagaa ggaaaataaa gaagatttag agcttgggag agagtttaaa atcaataatg
    14941 atagtatatt taaatttgtt gatttaatga atatcatggt agagtctatt tctgattctt
    15001 gcatgataaa tatagatatt actgatttaa aagaagctat tgtaggagat aaaggaatta
    15061 agtattcttt tgaagagttt gaagatacaa agtcatatag cgagatagct gacattttat
    15121 ttgacaggat ggaatatatt ggtgaggaat ttataagtaa gaaaggaatt gtatttgttg
    15181 aaggaagtcc agaattttct ataatggaat tgaatgattt aataagtaat attcagagca
    15241 aggtagaaga aagctatgaa gtaatatttt ctttatatat aaaggaaaat ttaaatggaa
    15301 atattagagt aggcttattg tataattagt atattttaga tattaaggag agtgtatatg
    15361 tctgaaaata taagaggtac agagtattgg ttaatggaag aattttttta tcaggaaaca
    15421 gacacaccac agatttttag agaagaaagc ataattgcac ttggatttga tataaaaata
    15481 aatttaaaaa aagtattcag tatggggaaa aaatcagaaa agtatattaa tgagaattgt
    15541 aatgataaag attttataaa aaagtttatg ttgattaaaa aaggagatta ttttttatat
    15601 aaagcatatc aaaatataga agaaaaagtt aaatgtaatg tgtctattgg catagttgaa
    15661 gaagattttg aaaatggata tgaattaagt catattttag ggcatacact accagtaaaa
    15721 tggatgcata attttgatac agtttcaaat tgtaaagtat atacaagtga attttataaa
    15781 ttaaataata atgatattag ctcatattta gaaattatta agtcacaaga taagaaatta
    15841 ttgagaaaaa gaatagaaga aagtgaaaat aataaatggt atttaaaaaa ctattattat
    15901 aaaaatgtag ataagtgtgt agatgatgaa gaattattta ttcagcccat taatctagaa
    15961 caattagaaa gtctaaaaaa tataaagaca gatgattata ttttattgta taatagcgag
    16021 aaagtaattt gtgaaaatga ccatacaatg aagagtctat ttggcgtatg tcaagttatg
    16081 gaagggttcg ataaagagaa agtaagtgct ttggagggtg gatatttttt tcctgttaga
    16141 tggatagagt atgaaaagga gaatatagac ggaaatgcat gggttatatt tgaaaataat
    16201 agtatagttg aaaagtatgt aaaaagattt actaataaaa tgccattaaa tactatactt
    16261 tatggaccac caggaactgg taaaacatac aatacaaaac agatggttta caatctagta
    16321 agtgaaacca gtctaagaaa cagatactat acaggttact tagattataa aaacaatgta
    16381 gaattttgta catttcatca atcatatgga tatgaggaat ttatagaggg tcttaagtct
    16441 gatggtgaag gcaattttaa gccagaagat gggatattaa aggatatatc tatagaagca
    16501 tgttataatg cattgaaatt tgaaaaaaag atgagctttg agttggaaaa agatagcttg
    16561 actttagaaa ctataaaaga caggaaaaag aaattagtat tagaaaatat agaatatagt
    16621 gagagtttta attttgaaga atcagaaaac tatgttttaa taatagatga aattaataga
    16681 gggaatgtat ctaaaatatt tggtgagcta ataacattat tggaagatga taaaagactt
    16741 actaaatcaa atcaaactat tgtcaaacta ccatattcaa aggaaaaatt ttctctacct
    16801 ccaaatttgt atatagttgg aactatgaac acatcagata aatcaatagc acttttagat
    16861 atagctttaa gaagaaggtt taattttgaa gaaattatgc cagattatga tttattgtct
    16921 attgtagatg gtatagatat tagaaaaatg ctatatacaa tcaataaaag gatagagttt
    16981 ttatatgata gagaccatgt tattggacaa gcttatctac ttgctgtaga aaatattaat
    17041 gatatagtag tagtatttag aagtaaaata ataccacttc ttcaagagta tttttattat
    17101 gattcagaaa aagtaggttt gattttagga ggaattggaa gaaatgaaaa agataaatac
    17161 attgtttata aagaaaaact tatagcttct gagttattta aaaataataa tgcctcaata
    17221 atggagagta aagttaacta ttgtataaaa caaaacatat ctagtgagga gctaaaaaat
    17281 atatatgagt aaaaatatat atgttaagga aacctatgaa tggataaggg taggtaatgg
    17341 tgaaaatgag cttacagaaa ttgaatatga aaaattgctt aagcatctag agaacaataa
    17401 tgatgtatta aaaagtaata taatagatat taaatacaaa aaacttagat ttataaacta
    17461 tgtaggcatc atatgttttg aaaatgtaat acttgaaata cttcctaaat tgtcattaag
    17521 tgataatctt gtaaaagata gagagatact attacagatg ctttctatat gtaataaaat
    17581 tccaattaca atgaatgaaa aaataagatt gagtttgaaa aattataatc tcttaaattt
    17641 ttttgttatg tatttcattg agagtatgca aacacagatg aaaaaaggta tttattttga
    17701 atatataaat aaaatagaga acttaaatgt tatgaggggt aaaatattat taagtacata
    17761 tgcaaaagaa aaaggcattt ctccaatgaa aataagatgt gaatatgatg agtatagtga
    17821 aaataatttc ttgaatcaag ttcttaaaaa agcatgtata tctattttat gtagaataaa
    17881 tgacaattca attcaaggca agattaaaaa aattttaagc tattttcaaa atgtagattt
    17941 aatttatata gatagaa
//
LOCUS       DAGSWI010000083        17917 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.083, whole genome shotgun sequence.
ACCESSION   DAGSWI010000083 DAGSWI010000000
VERSION     DAGSWI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.083"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            227..1156
                     /gene="glsA"
                     /locus_tag="MJF80_002516"
     CDS             227..1156
                     /gene="glsA"
                     /locus_tag="MJF80_002516"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBZ0281170.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1254..1817
                     /locus_tag="MJF80_002517"
     CDS             1254..1817
                     /locus_tag="MJF80_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281171.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2305..3141
                     /locus_tag="MJF80_002518"
     CDS             2305..3141
                     /locus_tag="MJF80_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBZ0281172.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3425..4420
                     /locus_tag="MJF80_002519"
     CDS             3425..4420
                     /locus_tag="MJF80_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBZ0281173.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4677..5081)
                     /locus_tag="MJF80_002520"
     CDS             complement(4677..5081)
                     /locus_tag="MJF80_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBZ0281174.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            6103..7017
                     /gene="queG"
                     /locus_tag="MJF80_002521"
     CDS             6103..7017
                     /gene="queG"
                     /locus_tag="MJF80_002521"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBZ0281175.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNISNKEFKR
                     KYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            7123..9360
                     /locus_tag="MJF80_002522"
     CDS             7123..9360
                     /locus_tag="MJF80_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBZ0281176.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            9558..10727
                     /gene="nth"
                     /locus_tag="MJF80_002523"
     CDS             9558..10727
                     /gene="nth"
                     /locus_tag="MJF80_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBZ0281177.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKEEINKKGMNKKDIIEKEEINKKGMNKKDIIEKEEINE
                     KGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKGETEKKTKKSSSNKATQ
                     KTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILSAQC
                     TDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELY
                     DGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQK
                     TEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            10921..11391
                     /gene="trmL"
                     /locus_tag="MJF80_002524"
     CDS             10921..11391
                     /gene="trmL"
                     /locus_tag="MJF80_002524"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBZ0281178.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            11427..12275
                     /locus_tag="MJF80_002525"
     CDS             11427..12275
                     /locus_tag="MJF80_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBZ0281179.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            12563..13567
                     /locus_tag="MJF80_002526"
     CDS             12563..13567
                     /locus_tag="MJF80_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBZ0281180.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFMGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            13693..14805
                     /locus_tag="MJF80_002527"
     CDS             13693..14805
                     /locus_tag="MJF80_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBZ0281181.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLLTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDFIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            15295..16056
                     /locus_tag="MJF80_002528"
     CDS             15295..16056
                     /locus_tag="MJF80_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281182.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            16059..16877
                     /locus_tag="MJF80_002529"
     CDS             16059..16877
                     /locus_tag="MJF80_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBZ0281183.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            17057..17659
                     /locus_tag="MJF80_002530"
     CDS             17057..17659
                     /locus_tag="MJF80_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBZ0281184.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
BASE COUNT         7404 a         1614 c         3288 g         5611 t
ORIGIN      
        1 tagattgcta aattattagg ttgttaaatt actggattat tagattacta aattgctagg
       61 ttgttaaatt attaaattat taagctattt aaactattta aattgtttga aaaatgaatt
      121 gattcttgta gaaaataatt ttaaagtata atttttttta acattgtttt attatagtat
      181 aaacttgtgc taaaatagaa tatataacat aatataagag gtgcatatga atacgttaga
      241 tgagacatta ttaaaagaaa ttataagttc taataagaag tatactaatt atggacaagt
      301 cgctagttac ataccagaat taaaaaacgc tagaagaaat gatttaggaa tttgtatcat
      361 agatagcgaa aataatcttt atagtgctgg aaactgtagc acaaagttta ctatacaaag
      421 tatttcaaaa ccaattgtgc ttgctatggc acttatggat aatgattggg aagacgtttt
      481 ttcaaacgtt ggaatggagc caagtgggga cccatttaat tcaataatga aacttgaaat
      541 taatgacaca aaaaaaccct gtaatccaat gataaatgca ggggctatag taaccacttc
      601 ccttataaat gggagttgtt tagaagaaaa agaagaaaga atgcttagtt tctttagaaa
      661 gttagcaaaa aatgataata taggaataaa ctatgatgtc tataagtctg aaaaaatgac
      721 tggagataga aacagagcta tggcttactt attgaaaagt gatggattta tcagagggaa
      781 tgtagaagat gttctagacc tttattttaa acaatgctca attgagatag attctgtaga
      841 tttagcgaga attggaataa atcttgcaaa ttatggagta gatatagaaa atggtgagca
      901 tttaatgagt gaaatggttt ctaggattgt caagacattt atgatgactt gtggaatgta
      961 tgatgcttct ggggaatttg ctattaaagt tggcattcct gcaaaatctg gagttggggg
     1021 tggtataatg gcttctgtgc cgggacgcat ggggattggt gtttatggac ctgcgcttga
     1081 caaaaaaggt aacagcgtcg caggagttaa agttttagag gagttgtcaa ataaactcaa
     1141 attaaatatt ttttaaaata ttaagaaagg atttttttga caacaacata taatttaata
     1201 tataattaaa ttgtaaagaa atatgaatta aataaatttt ttaggagggg tcaatgaaag
     1261 aggtattggt attacgagat ttggagtgca taaaggctat tgcgcatcca cgtagaatag
     1321 atattctaaa agcatttgac aaatcgccat tgtcagcaaa acaattatct caattattgg
     1381 aagaacctca tgcaaaaata aactaccata taaaaatgtt atacaaagtt ggtattttag
     1441 aattagttga ggaaaaaata aagtcgggga ttgtggaaaa gtactattat ccaagtgcaa
     1501 aaaatgtggt tatagggaat aggatactta atttttcttt agacaatggg gaagaaaaag
     1561 aagaattgta catatcaaaa tttgaaaata tgagtgaagt tttttataag gcagcagagg
     1621 aagatgtttt agaaaatgaa aatatagttg attatcatga tatttctctt actcatgatg
     1681 aattagttga acttagtgac actatgaagt caaagataga tgaaatttta aataaaagac
     1741 aacacaatgt tgaaggttct aagtatgata tagctatggt tatagttcct actcttgagg
     1801 aggaatgtcc tagttagaga attaattatg gattaattaa agatataata ttagagattg
     1861 aacatcaaaa tttacttaat aatcaaaaaa tagtttttgg tatgggtaaa acaatgattt
     1921 ttaatctcta tttttttgtt tttttatacc tattgtattt gtaaataata tgtatagtgt
     1981 aagatgtata cactatctaa gaagtgctta gataatatac aaagtatgta agattataat
     2041 atttgaaatt gattaaaatg ggtataatta aagtgtaaat attaaaatta aaaattaata
     2101 taaattaaat caatgctaga aatatgttaa aagaggaatg agattagttg ggatagaaca
     2161 tcccttagag cttacgaatt tttagtgaaa aagtactatc aaataggaat ctccatcttc
     2221 atatgttggc taaatgtagt taaggtggag tagttcaaaa ttaaaattga gtacaagtag
     2281 tagtacaaat aaaaggagat taatatgatt aaattaggaa cacatctttc aatagcaaaa
     2341 ggatttgcta atgcggctga gacagctgta tatattggtg caaatacatt tcaatttttt
     2401 agtagaaatc caagaggagg aaatgctaaa gaatttgatg aaaaagatat aattaaattt
     2461 caagaaatta gaaaagaaaa taattttgca ccactgcttg cacatgcacc atatactatg
     2521 aatctgggtg gtacaaagga tgatgtatac gaatttgcaa caagggtgat agatgaggat
     2581 ataagaagga tggactcatt aggaattgaa tacatgtgtt ttcatcctgg aagtcatgta
     2641 ggtggtggtg tagattttgg catagataga atagtaaaag gattgaacaa tgccataaag
     2701 ggtgatgaaa atataactat acttcttgaa actatgtcgg gaaagggaac tgaaataggt
     2761 tttaaatttg agcaattaaa aagtataata gacaaaattg agcataaaga aaaaataggt
     2821 gtgtgtcttg atacatgtca tattttttca gctggttatg atatagtgaa tgatttagat
     2881 ggggttttgg aagaatttga taaggttata ggcatagaaa gattgaaaac tgtccatcta
     2941 aatgatagta tgatgccatt tggagacaaa aaagacagac atgctcctat aggggaagga
     3001 aagataggat taaaagcatt aattgatttt atggaacatc ctagtctaaa acatttacca
     3061 tttttcttag aaactccttt tgatgatgaa ggtcacaaga gagagcttaa aatgataaaa
     3121 gaaatactat ctagtaaata aacctttatt aaaaatatgt gaaatactcc accctcaaca
     3181 taattagggt ggagatacaa tcatgaaatg tgataattta aattaatgga taggaataac
     3241 gaaaattgta tacaaattaa ctgggtataa agtatgatta tattatgaat agactgatat
     3301 aaaatataat tatattatga atagtatgta aatacattgc aaagattctc ataatttgtt
     3361 gtataattaa aggtgacata tttatattat ataaaaaatt attaaaagtt aggtggggaa
     3421 agatatgaga aatttaggca atactaacat gaaaataaag agagttggct ttgggggcat
     3481 tccaatacaa aggattactc aagatgatac taacttagtt ataaatgagt tagaaaaaca
     3541 aggaataaac tttattgaca gtgcaagagg gtatacaata agtgaggaag caatcggaat
     3601 tgccattgaa ggaaaaagag ataaattttt cttagctact aaaagtatgt ccagagatta
     3661 tgattcaatg aagagagatg ttgaaataag tttaaataat tttaaaactg attttataga
     3721 tttatatcaa ttccataatg ttaaagaaga ggagtatgat aatcttttta aagataaaat
     3781 ggcttatagt gcccttttag aggcaaagga gcaaggtaag ataaaacata ttggtataac
     3841 tagtcacaat ttaaatacta tagaaaaagc tatagaagat ggaaaatttg acactataca
     3901 attcccttat aatatagttg aaggacaagc agatgaagtg tttaaaaaag ctcatgaaaa
     3961 aggaattggg ataatagtaa tgaaaccttt agctggaggt gctttagaca atgcaacact
     4021 ggctattaaa tatatactat ctaaagagta catagatgta gttataccag gtatggagag
     4081 tgttgaacag gttagacaaa atgtcgctgt acttgaaaat cttgtgctag atgaaaaaga
     4141 taataaggaa attgaagaaa taagaaattc tttaggtaaa aaattctgta gaagatgcga
     4201 atattgtatg ccttgtgctg taggaataaa tataccattg agttttttat gtgaaggtta
     4261 ttatactaga tatggattaa aagagtgggc taaagaaaaa tatgaggtta tggatgttaa
     4321 acctactgag tgtattgact gtgggctttg cgagagtaga tgtccatatg aacttcctat
     4381 aagagaaatg cttaagactg ttgttgaaaa gctaggatag attttattta ttgaaatcgg
     4441 tgtattaaat ttactccgat ttctttttat tagatgtggt cttatataca aatattaaat
     4501 ttacatagtt atagctatta tataaattta atatttgttt aatacttatt aaatatttac
     4561 ttaatgcttt ttaaaaacat ttgtttgagt atagacataa cacaattatt attgttgtat
     4621 tagtatagtt tcaaatactg gagtatcaat ataatgtcga ataacacata aaatctttat
     4681 ttttcaaaaa tattatagga tatatcgtat ttagatactg gataaagttc aatttcaacc
     4741 ctagggttat ctttatctat aaaatcaaat aaaagtactg gcttaagttg tttatcattt
     4801 tttattattc cacttttttc aataccatca caaatacttt ttggataatt atgtaaatcc
     4861 cccattctct tatttgggaa gaataatttc aagactgtta ttagattttc ttcgacagtt
     4921 tccccttgat actgttggtt tataaaacct gcaatcatgt tttcgtatgc aagatactta
     4981 gaatattttc cctttgaagg catccatgct tgaccattta tgttatgaag cttaaaatta
     5041 gatttactaa taggtctacc tggaattgtt attttaatca aatttttcac catccttata
     5101 cagtatttat tataatctgt atatgattta aaagcaaatt gttatttaaa gtgtttttac
     5161 agagcttttt tgtaaaattt tccactttag atgtagtaat ataagataag tattttgctt
     5221 acaaatttta aaatttgata tatgatggac atattcatat tagtctatta taagaccttc
     5281 ataatattaa taagtatagt ttagtaaaga gagtaaaaag aaatccatat aagtcaagta
     5341 aagatttaat aaatggcaat gtaaatggga tattaattat aagaaaaaat caatcttttt
     5401 gcttaactat agaaagaatg tcaaaatata actaatgata tttgaagttt tagagtttat
     5461 attaaaaggt aagaaggtta agtgggtaaa aaaggttgaa tagattaaaa aggctaagta
     5521 tctaaaagag ttaagtaggt aaaaaagtta aataagtaaa tgaaaaaggc taagtatcta
     5581 aaagagttag tagttaaata agtaaatgaa aaaggctaag tatataaagg agttagtaga
     5641 taaaaaaatt aaatagataa aaagattaag tatctaaaag cgttagtaga taaaaaaatt
     5701 aaataaataa aaaagactaa gtattcaaaa gagttagtag gtaaaataat taaatagata
     5761 aaaagactaa ttatttaaag gagttaagta gataaaaaag ttaaataagt aaatgaaaaa
     5821 gacgaagtat ctaaaagagt tagtaaataa aaaagatgaa gtatctaaaa gagttagtag
     5881 gtaaaaaact aaatagataa aaagattaag tatctaaaag cgttagtagg taaaaaatta
     5941 aataggtaaa aagacgaagt atctaaaaga tttaagtagg taagaaaatt gaatagctaa
     6001 aaagattaag tatttaaaag agttagtagc tagaaaaggt taaaaagact aaaaaaatta
     6061 aataaattta aaaagtcaat tagattaaaa aggaataatt ttatggataa taaggaatta
     6121 aaagcatttt gtaatttaat aggattaaac tgtgtaggag tagcaggggt agataagtat
     6181 gataatttag aaaagatact taaggacaga caacaaaaag gttatttaac tggtatggaa
     6241 gaaccaataa ttgagaacag aattaaccca agacatataa tgaaggatgc taactctata
     6301 atagtctgtg catttccata tcatataaat agacaagaaa agaataaaaa ttcaaattta
     6361 tcaaaatatt gtcatggaaa ggattatcac attgtagtaa aggacttttt acaacaaatt
     6421 tgtgattata tttctaaaac agtaacagat tttaaataca agttatttgc tgataatgga
     6481 ccattagttg acaggtattt agcttattta tcaggaatag gatattttgg aataaataat
     6541 aatatcataa ctgatgaata tggttcttac gtatttatag ggtatatagt aaataattat
     6601 aaatttgaag aagataaacc actagaaaaa agttgtttta aatgtggaaa gtgtgtaaaa
     6661 tattgtccgg gaaatgctat attgggaaat tatgatatga accccaaaaa atgtttatca
     6721 tatataactc aaaaaaaagg agatttgtca gagaaagaaa aagttgtttt aaagaatggc
     6781 aaaaaggtat ttggatgcga tatttgtcaa gaagtatgtc cacataatgt tgaaatacca
     6841 acaactcata ttttggaatt taaagaaaat ataatagatt atttgaatta tgatgagata
     6901 caaaatattt caaataaaga atttaaaagg aaatatggag atagagcttt tagctggaga
     6961 ggaagaaata taataaagag aaatatggaa ataattttag ataagtatga tgactaataa
     7021 taagagtctg tttcatatac tttaatgtgt atttacttta acacttgttg tgtaaaagtc
     7081 atattatatc attaaagtta aatatgggga ggtgggggaa tattgaaata taataataca
     7141 tctgaaataa atccactaga ttatatattg ggtcaagaaa gagctgtaga agctatggaa
     7201 ttagggttga aaataaataa tccagcgtac aacatatata tagctggtga acctggaact
     7261 ggtaaaagta cttatgcctt aaaggtttta aatgagtatg catccaaaaa aaatacgcat
     7321 aaagattggt gttatatata taattttgaa aaccctagag agcctattat agttgaatta
     7381 gaaaaggggt ttggaagaga acttaagaaa gatatggaga aattaattga aagtctttta
     7441 gaagatttta aaaatgcgtt tgaaagtgaa aattttgaaa tagaaaaaaa taaacttctg
     7501 gatgaatatg aaatagaaaa agatatgctt ttgaagcaaa ttaaaaaata tggagaagaa
     7561 aaaggattta aattaaaaca gagtaaaatg ggaatagttt ttatcccttt gaaagaagag
     7621 gaagatgaaa gtgatgagtc tgatgaagag ttttacaaag ctaaaagaga actagaaaat
     7681 atggcgatac aggtcgtata taaaattaga aacttagaag aaatagctga aaaagcagtg
     7741 ttagagcttg aagaagagat agctaaactt gtagtagaac ctcatatcaa gagattgtgt
     7801 gaaaaatatg aaaattatga taagattcaa acttatctgg aaaatataaa gaaagatata
     7861 atagaatata tgtatttatt ctatttagat gaagaggagt taaaagataa gtatgataaa
     7921 gagcatttta ttaaatataa aattaaccta tttgtagaca atggaagctc aaagaataaa
     7981 gaatcagcac ctgttgtagt agagataaac ccaagtccag caaatctatt tggaaaggca
     8041 gagtatgatt atgcaaatgg aaatataaag actgatttca caaaactatt atcaggagca
     8101 tttcataggg ctaatggtgg ttatttagta ctttatgcag accaattact aaaatataca
     8161 atgtcttggg aaattcttaa aaagactttg cagactaaaa aagtaatatt ggaaacacaa
     8221 acagcaatta aaccagaaaa tatgccattg gatgttaaat tagtactgat tggaagccac
     8281 tatatatatg atattttata tagatatgat gaagattttg aaaaatattt taaagtattt
     8341 gtagactttg atagtgaaat ggataaaaat gaagataatg aagaaggaat agcaagattt
     8401 atagcatacc aatgtgataa gaataattta aggcatttta ctaaatcagc agttgaagag
     8461 gttattaaat ttagcactag attatctggt gatttagaaa aattatcaac taagtttaat
     8521 aaaataatgg aagttgtaat agaaggaagt gcttatgcag agtttagaaa ttcggagtat
     8581 acagagcagt gtgatgttaa aaaagcaata agtgaaaaga gaaaaagaat caatagagtg
     8641 gaaacacata tggatgaatc aattgaaaat ggatttactc taatagaaac agagggaaga
     8701 aaagtaggag tgataaatgg tttgtcagtc ttaagtacag gagaatactc ttttggtaga
     8761 gcttcaagga ttacagcgac cactagccca ggaagtaaag gcatagtaaa tatagaaaga
     8821 gaagtaaata tgagtggctc tatacataac aaaggagtac ttatattagg tggctatctt
     8881 tctgagaatt ttgcacagga tttactattg tcactaaatg catatgtttg ttttgaacaa
     8941 aattatggtg gaatagatgg agatagtgca tcttctgcag aattatatgt actattatca
     9001 tctcttagtg gagtatcaat taaacaaaat attgctgtta caggttctat aaatcaaaag
     9061 ggtgatatac aagtagttgg tggtataagt gaaaagatag aaggatttta ttctatatgt
     9121 aaaactaaag ggttaaatgg tgagcaaggc gtcataattc ctagaaaaaa tcaaagaaat
     9181 ctcgttttat cagatgaagt taatgatgca gtgcgagatg gaaaatttaa gatatatact
     9241 gttgaaagag tagaagaggc tatagagata ttgacagatg ttaaatttga agaaataaag
     9301 aaattggtaa aagaaaaatt aatatctttc agcaagatac aaactgtgtc aaaagaataa
     9361 gtttattata gaatttaatg ggtatttatg tataataaaa agcaagaact ttaataggtt
     9421 tttgcttttt ttgtaaatat atataaatta aattcttatt ttgtatgttt tatatattga
     9481 tgtttatttt tttgttgata tgattaaact atttataaat agcgttttat aaattggtat
     9541 tttgaagggg gataattatg aaaaatgtaa atctaaaaga gataaatgaa aaagatgtaa
     9601 ttttggaaga agagataaat aaaaaaggaa tggataagaa aaatataatt gagaaggaag
     9661 agataaataa aaaaggaata gataagaaag atataattga gaaggaagag ataaataaaa
     9721 aaggaataga taagaaagat ataattgaga aggaagagat aaataaaaaa ggaatgaata
     9781 agaaagatat aattgagaag gaagagataa ataaaaaagg aatgaataag aaagatataa
     9841 ttgagaagga agagataaat gaaaaaggaa tagataaaat gattggtaaa aaagatgaga
     9901 taattgatga gaaagatata gagaatttta tggatattga agaagataaa aaaagtatca
     9961 aagtaagtaa aaaaagtact aaaaaaggtg aaacggagaa aaaaacaaag aagagtagtt
    10021 ctaataaggc tacacaaaaa acaagtaaag aaactaaaag taagtctaaa attaaaaatg
    10081 agtcagcaaa tgaaaaagac gtaaataaga ttttagacga gttagaaaaa ttgtatccag
    10141 atgctaaatg tgagttaaat tatgggacag cctttgaact tttaatagca actattttat
    10201 cagcacaatg tactgatgta agagtgaata aagttacttc agagttgttt aaaaagtaca
    10261 acacagctag agactttgct aatttgagca ttgaagaaat tagtaaagag ataaagagtt
    10321 gtggtcttta caaaagcaag tctcaaaaaa taaaggatac atctgaacag ctatgtgaat
    10381 tatatgatgg tgaagtacct gatagcttag aaaagcttat aaaattacct ggtgttggaa
    10441 gaaaaacggc aggggttgta cttagtaacg cttttaatca tccagcaata gctgtagata
    10501 ctcatgtatt tagagttagc aatagaatag gaatagtgga cgagccaaat cctcaaaaaa
    10561 cagaatttgc acttatggaa gctataccaa aagaaagatg gtcacactca catcatgtat
    10621 taatttttca tggaagaaga atgtgtaaag ctagaaatcc agaatgtgca agttgcccaa
    10681 taaaagaaga ttgtaattat tataaagaac ttaatgaaac gaaataactt ttagaaagta
    10741 agttgtacaa gagggatttt ctgatataat aatacttgtg aaaatgttag ataaaaaact
    10801 attatataca tgaaataaaa attattatta acattatttt atataatagt ataatattat
    10861 tgttattctg tttataaaat aaaatttgat aaataaaagc gatacttagg gaggattaaa
    10921 atgtcattaa atatagtatt agtagaacca gaaatacctc aaaatacagg aaatataata
    10981 agaacctgtg cagcaacagg ttcaacttta catttagtaa aaccactggg tttttcattg
    11041 gatgataaac acctaaaaag atgtgggctt gactattggg atatagctga tatacaatat
    11101 tacgatagct ttgaagaatt acaagaaaag tatccaaata gtaaatactt tttttctaca
    11161 acaaaggcta aacagtcaca ttcagatgtg aaatatgaag aaaattgttt tatagtattt
    11221 gggaaagaaa caaagggatt accagaacct ttgttgaaag caaatatgga tacttgtatt
    11281 agagtgccta tgttggatgt tgaaaaagct aggtcattaa atttatcaaa ttcagttgct
    11341 attgtagtgt acgaggcttt aagacaaata ggatacccaa atatgaggta gtttcaacaa
    11401 gcctaaatga gaaaggagca tacttaatga aaaatctaaa gataatctgt gacagtttat
    11461 cggacgtgcc aaaagatttg ttagataaat atgatataga tatagtttca ttaacagtta
    11521 tactggagga aagagaatac aaggatagaa ttgatatatc tggagatgaa ttttataaaa
    11581 ggcttagaga agaaaatgta tatccaaaaa cttctcaagc tacttatgcc caatttaagg
    11641 aaatttttga gaaatataca aatgaggata gagagatact ttatatagca gggtctgcca
    11701 cagctacagg tacgtatcaa agtgctgtaa tggcaaagaa tgatgtagat ggtaaaatat
    11761 atacatatga ctctaacttg ctttgtatgg gaactggttt acttgtaata aaagcaggag
    11821 aacttgcaag tgaaggaaag tctgttgctg aaatattgcc tgttttggat gaattaaaag
    11881 agaaggcttt tgttatattt tcagtagata cattggaata tttacaaaag ggaggtagaa
    11941 tatcatctac taaggcagca ataggaagta tattaaatat aaaaccgatt cttgaaataa
    12001 gagatggatt agtttcgcaa atatctcaag taagaggaaa gaagaatgta atttctaaga
    12061 tgatggaatt gataaaatca aattgtggtg atgatttatc agaccaaatt gtgtatatag
    12121 gatatagtga tgattttaag gaaaaagaac aattatctaa ggttatgcaa gatgaattaa
    12181 gacctaaaga aattagattt ttccaaattg gtgtgtgtat aggtgcacat gctggtcctg
    12241 gtgttactgg tatcatatgc ttaaaaaata aataatatat ttttggatga cattatttat
    12301 ttttttatta aattaggata gaatattaat tgttgagatg ttaaattgga tttattttgg
    12361 atgaaattaa gtgttgatat attagattta gtttgtaggg aagtgtgtaa atagataaga
    12421 atctattgca cactttttta tagtaagatt tattaaaaaa atttcaaaat gtacttgtaa
    12481 attgtatata aaaaaattat aaataggatg taaaagtaaa taggatataa aaatatcgta
    12541 aatgtatgag gagtgaaatt taatgcgttt caaaagaata ttgatattga tgttaactgt
    12601 agcaatgata gctggaatgt tggttgggtg tgctaataac ccaagtaata atcctagctc
    12661 tagcaaagat aataaaagtg atataagcga taaaagtaat tcaaaaaaat ctgtatcaat
    12721 gatactagat attgaaggaa caaataatga agctatgaat aattcagctt tattagcatt
    12781 aaacaatgct caaaagaaac ttaatataga tacaaacaaa gtggaatcag atgattcctc
    12841 aactttttct aatagtatag atattttatg taatgacaat tacgatttaa ttatagctgt
    12901 aggagctaga tttgcaaaac cattggagat ggtagccaag aaatatccaa agcaacaatt
    12961 tgcaattata gactatgagt atgacaagca accttctaat ataacttcaa tttcttatga
    13021 agataataaa tctggatacc tagctggatt aattgctgga aaaatgactg aaagcgataa
    13081 ggttggtttt atgggaggaa caaagagttc tagtagagat aaatttgaat caggttttag
    13141 agagggtgtt aaattttcta atagtagtat aaaggatatt tcagttgagt atgcagatgt
    13201 ttttaaagat agcaaatcag tagaatctat agctaaaaaa atgatggata atggagtgga
    13261 tattattttt tctactactg aagatgattc aaaagctgta atagatgcag taagggcaaa
    13321 aaataaaaaa gttatagcaa cgaataaaga ccaacatgag ttagctccag aaaatgttat
    13381 aagttctatt gtaaaagatt ttgagaatcc tacttataat ctgatacaaa gttttgtaaa
    13441 aggaaattat aaaggcggaa aggttataga aaacacagtt aagtctggaa gtactggact
    13501 tgcccaaaat agttctcaaa atattcctcc agatgtgtta gaatatgtta ataagaacaa
    13561 taaataatct actataatat ccaagaatac ttatgagttt agttatgagg tgaattggat
    13621 gttataatag acatatatag aaagacatat gcagaggtag gtcagaccta tctaaatcag
    13681 ggagtgaatt ttgtgaaaat aaaaaggata ttgatggctt gctttgtagt agtttttaca
    13741 atttttatat tagctatatg taattttgat tttgggtttg ataaagtaaa taagaataat
    13801 tctaatatag atagtgctaa atcgaaacag agtgagtcta aaagtttgct aacttcagca
    13861 gaaaaggata aatcaaatag taaaagtaag aataaagaag atgacacaga agatgattca
    13921 tatgtaagaa aaactattgg agtattgtta gataccgaaa acttaaataa cgaatcatat
    13981 aataacaatg tactatcagc cttaaaaagg gctgacgaag attttgatgt taggataaaa
    14041 cttgaaaaac caaagacttc taaaagtttt aaagagagta tagaaaaact gcataagtat
    14101 gaaccagatt ttataatagc agttggagcc agatttgcag agccaattaa agaagctgct
    14161 actaaatatc ccaaacagca atttgcaata gttgactata cttataaagt tcagccagct
    14221 aatgtaattt ctgtatcatt tcaagataat gttactggat atttagcagg acttattgca
    14281 ggtaaaatga ctgatactgg gaaagtcggt tttataggag gagttgaggg ttctagtaga
    14341 gataagtatg aatctggatt tagaaagggg cttaaaactt caaataaaga tgctgagtta
    14401 ctagttaagt attcaaatat atttgaaaac cctgaatcag ctagaactat tgcaaaggaa
    14461 atgagagatt ctgatagaga tgttgtattt tcagctacag aagatgatag taaaagagct
    14521 attcaggtag caagagaaaa tggtggtaaa gttatagcta ttaataaaga ccaatacgat
    14581 atggctccag ataatgtaat aagttcaatt ataaaagatg tagaacaacc tgtatatgaa
    14641 ctgataaaga acgttgcaaa agatggtttt aagggaagta aagtaatgga atttaaaata
    14701 aaagatgggg aacttggctt gacttcaaag agcagtagaa atataccacc agatgttttg
    14761 gaatacataa aaaaagagta taacaaagtg aaagatacat attaattatt gatgtagaag
    14821 attataaaaa tatattgctt aagtttagat tagcaataaa aagatatatt tattaaattt
    14881 gtgctaactg tacaaaatat attgattaag tttagattag tgataaaaag atatatttat
    14941 taaatttgtg ttaacagtat ggaatatact gcttaagttt agattggtga taaaaggata
    15001 tatttattaa atttagtcta actgtacaaa atatattgat tgagtttaga ttagcaataa
    15061 aaagatatat ttattaaatt tgtgctaact gtacggaata tactgcttaa gtttagatta
    15121 gtgataaaaa gatatattta ttaaatttga accaactgta caaagctata ctaattaagc
    15181 ttagaataac tatataaaat tataacaata agtgtgtggg cagaaaaatt ctgctacaca
    15241 ctttatttat attcacataa aaaatttatc aaaaaaaaga aaaaggagta aaaaatggaa
    15301 tttacaagag aaccatttat actagatgac gatttgtgtc tagctttggt ggacaaaaga
    15361 attagttatc taatggaaaa tgaattaaaa ttaagaaata tagagataat aaaaacaata
    15421 gaatgtaaag aattatatga aagcataaaa tatcacccgg atatatgtat ttgtaattta
    15481 ggaaaaggag atataattgt tgcgccgaat gtatataatc aatacaaaga gttattaagt
    15541 agatataaat ttaatataat taagggagaa gctaatttat taaatagata cccttatgat
    15601 ataccttaca atattgcgat agttggtgac tatgctatac acaattttaa gtatactgac
    15661 aaagcaatat tagattatat tgaaaataat aaacttaaaa agataaatat agagcagggc
    15721 tattctaaat gctcaatctg tatagtagat agaacgtcta taataacatc agataaaggt
    15781 atttggaaaa gtatgaagaa tacagaaata gagtgtttat tgatagaaaa aggtcacata
    15841 aatttatttg agatgaatta tggatttata ggtggatgta cagggttgat ttctaaggat
    15901 aaactagctt tttgtggaga tgttaaaaaa catcctgact atgaacgtat aaaaagtttt
    15961 gtagaaagta aaaacaagga gatagttaca cttagctgtg aaaatttact agatataggt
    16021 tctatagttc ctctcatgac aagaaaggag cgataacttt ggttgagaag gataatattt
    16081 cttccatgaa atttttgagg aataataatg taaacgatag attaatatat acgaatgaca
    16141 ttgtagaagt agtgatagat tctgcaaagc aagaagtgtt aaataaaaaa atacttaatg
    16201 gtactaaagc tgaacaaaga gctttaaata ttgatgagat atcaacggaa ataactagta
    16261 tgataataag tataatgaaa gagaaacttt tgaaagaata cattctaaaa tgctatgggc
    16321 gtacatatcc tgaagataaa aataatatat acatatattc actgaacata tttgctaaaa
    16381 aagagatttt tatgagagaa acagtattta ctagggtgag aaattatata gaagaaaatg
    16441 attttataaa tgtagaaggt tttattagat ttagaatgag agaatttatg aaatatattt
    16501 ctgctatagg tgatattgca gttgaggagt atttaataaa gaaagaccaa gatgaattta
    16561 taagagtttt aaaatacttt atagatactc aagaagaaaa gattgatttg cttaaagttc
    16621 atataatgga agataataca tttgttttat acgataaaaa tggaaataag atagacagta
    16681 tagatgatga agaaattata aatatggtaa taagagaaaa tttaaattat gaggactttt
    16741 taataagtac cttattaact ctatgtccca aaaaaataga aatattggat ttgttaaata
    16801 ataattgctc aaaggaaatt atagatactg tagaagcaat ttttgaaaat aaagttagta
    16861 taattatgga aaactagaat ctaagcatat aaaagctatc catataaaat taaaaaaatt
    16921 ctttttaaat cgacacaaaa gacttataat tgtatataaa gctatagtat aactagagtt
    16981 attaagtata aagaatatct acttgattaa tgcaatatag ctggcaaata tacaattgat
    17041 aaggagtaat tttataatgg ataatttaat taggatactg atattagctg ttattggtgg
    17101 atttataggt tatgttacga atgtagttgc tataagactg atatttagac caatagagcc
    17161 tattaaaata cctattttaa atatagaaat agtaggtctt ataccaaaga gaagagcaga
    17221 aatagcagca aatgtaggag aaataattca agaagaattt ctttctatgg acgaaatact
    17281 tgcaaatatc attactgacg aagataagga agaagttgta agatacataa aagcaagggt
    17341 aaaaattata atacatgaga aagtttcatt tatcccgagt ggtataaaaa acatgataca
    17401 agattatcta ggagagataa ttgagtctga agttaagcaa agtatagatg aattgagtaa
    17461 aaatataata aataaagcaa atgaacgtat agatatacaa aaaatggtcg aagataagat
    17521 taatgaacta gatttatatg aattggaaga gataataata agaatagcta agaaagaatt
    17581 aaaacatata gagtttttgg ggcttgtctt agggttttta ataggtatag ctcaagggtt
    17641 aattactatg tttatttaaa tatatgagaa gtagcattta tttaagtact aattatattt
    17701 acctatttga gtgaattact tattaataac atttagatat taattgtatt tacctatttg
    17761 agtaaattac ttattaatag catttagata ttaattgtat ttacctattt gagtgaatta
    17821 cttattaata gtatttagat attaattgta tttacctatt tgagtgaatt acttattaat
    17881 agcatttaga tattaattgt atttatttat ttgattg
//
LOCUS       DAGSWI010000084        17673 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.084, whole genome shotgun sequence.
ACCESSION   DAGSWI010000084 DAGSWI010000000
VERSION     DAGSWI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17673
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.084"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..617)
                     /locus_tag="MJF80_002531"
     CDS             complement(<1..617)
                     /locus_tag="MJF80_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBZ0281185.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKM"
     gene            complement(931..3069)
                     /gene="vanT"
                     /locus_tag="MJF80_002532"
     CDS             complement(931..3069)
                     /gene="vanT"
                     /locus_tag="MJF80_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBZ0281186.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(3087..3893)
                     /locus_tag="MJF80_002533"
     CDS             complement(3087..3893)
                     /locus_tag="MJF80_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBZ0281187.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3890..4990)
                     /gene="vanG"
                     /locus_tag="MJF80_002534"
     CDS             complement(3890..4990)
                     /gene="vanG"
                     /locus_tag="MJF80_002534"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBZ0281188.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(5251..6333)
                     /locus_tag="MJF80_002535"
     CDS             complement(5251..6333)
                     /locus_tag="MJF80_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0281189.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            complement(6383..7084)
                     /locus_tag="MJF80_002536"
     CDS             complement(6383..7084)
                     /locus_tag="MJF80_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBZ0281190.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(7518..10049)
                     /locus_tag="MJF80_002537"
     CDS             complement(7518..10049)
                     /locus_tag="MJF80_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBZ0281191.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(10157..10774)
                     /locus_tag="MJF80_002538"
     CDS             complement(10157..10774)
                     /locus_tag="MJF80_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBZ0281192.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(11136..12020)
                     /locus_tag="MJF80_002539"
     CDS             complement(11136..12020)
                     /locus_tag="MJF80_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBZ0281193.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(12211..13461)
                     /locus_tag="MJF80_002540"
     CDS             complement(12211..13461)
                     /locus_tag="MJF80_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0281194.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(13683..14447)
                     /locus_tag="MJF80_002541"
     CDS             complement(13683..14447)
                     /locus_tag="MJF80_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281195.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(14441..15166)
                     /locus_tag="MJF80_002542"
     CDS             complement(14441..15166)
                     /locus_tag="MJF80_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281196.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(15168..15536)
                     /locus_tag="MJF80_002543"
     CDS             complement(15168..15536)
                     /locus_tag="MJF80_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0281197.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(15712..17256)
                     /locus_tag="MJF80_002544"
     CDS             complement(15712..17256)
                     /locus_tag="MJF80_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBZ0281198.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         6082 a         3106 c         1764 g         6721 t
ORIGIN      
        1 atcattttat catctttatc attgtgatgt tttttatgat ggaaatcata attatgattt
       61 tcaagatttt tatttactct atttccaatc ttccatgaca aaaacaccaa taatataaat
      121 gatgctatct ttaaaggact tgttataaaa ccttctatat cacttgccac gtaacttata
      181 actataaaca ttacaaattt tcctgccaac attgctggca caaaatcttt taaatctttt
      241 ttaccaaaag ctgatgcgat agtaactaaa caaccgggca taaatggaca ggcatacgct
      301 ataaataata acttaaatcc ctgtttatcc atccaactta ttgctttttc agttttacta
      361 tttctcaact tattaaaaag cttactatca ttaaatctgc ttattaataa gaataatgaa
      421 gttgtcccaa gtcctgagcc aatccatgat aacaaacatc caataaataa tccttgtatt
      481 gcagcatttg ctgtaactat agcaacaagt ggtaaggctg gtataaagct ttctataaat
      541 gcacttaata agccgattat tgtggctaac acccaataat tttgagtcaa atcaagtagt
      601 tcttgtatat actgcatatt gcttttcccc ttttttaact ttaactatta ttatatactc
      661 tatatgttta ttatatacca atctcaaaaa atgaatctaa tattaaaaag taaataatct
      721 atataagttt acagttaagg tataattatt ttattatata ttataacaaa aaaggtgatg
      781 tttcaaaata aaaatctgaa acatcacctt tttataactt aaattgtcat gtaagttata
      841 aaaaatatat attagacata atattactaa aaatgtctag tcagagtaaa ataaattaca
      901 cagtacaaca aatattacta tttaattgag ttacttcttt ataacattta accttcttcc
      961 tactctgctc aataattcat tcgatatact acatgagtta tttgcaactt cagaagcaga
     1021 taattcagat aaattatctc ttcctattat tattgccgta ttaccaactt ctacattagg
     1081 tatatctgta atatcaacag caagttggtc catgcaaatt cgacccacta ttgctgctct
     1141 acaaccattt attattacgt aactttttcc acaggataaa tttctaggta taccatcggc
     1201 atagccaata gataatatag caattacgct atctctgctt gcaataaaga ctcttccata
     1261 tccaaaacta tcatcttttt gtatttttct aataagaatt acttgtgatt ttaaggataa
     1321 aacaggtcgt aaatcaagtt gcaatttagt cgtatcattt ggagaactta atatcccgta
     1381 taatgcaatt ccaattctta cataattaca cttaagattg ggataattaa aaaaaccata
     1441 actactttga atatgtactt taggaatttt tatacctttt tttaacagaa aatcaattaa
     1501 cttataaaaa cattcaattt gtttatttgt aaagtctaca tcttcatctt tcagactatc
     1561 tgaaacacac aagtgtgtaa atatgccaca tatatctaag tattttaatt taaatgcctt
     1621 taaaattgac ctcacatcat taacatcaaa acccagtctg tgcatacctg tatcaatttt
     1681 aatgtgtgat tttatgttgt atttctgttt gtttaaagag atagcatgat taaggtcaat
     1741 aacactctgt gtaagattgt atttatgaag ttcctttgcc cttattgggt tagtatatcc
     1801 aagaatcaat atttctccta gtattccata tcgacgtaaa tcaatcccct catctattgt
     1861 tgctactgca aatgctttta cacctatttg gtttgcacaa gtcgctactt caaaagcact
     1921 atgaccatag gcattagctt ttacaactgc catcaattca caatcttctg gcatagcttt
     1981 ttgtatcatc tttacattat gttccaaatt atttaaatca atttcaatcc aagctctatt
     2041 ggtatttacc ttatgttctc ctttcaagtc tttttttaga ggtttaagaa tcaatataat
     2101 aattatagaa gctataaaag atgctatact taccactata taatgaataa ggctattgtc
     2161 aaccaagaca gtttgtattc cgaatatttt agaaaacata cgaataacaa caatcatcat
     2221 aggatgtata atataaataa ttaatgatga tgttcgcaat aacctagctc gagaaccttt
     2281 caaaaatgtt aaggctgtaa ataagaaata catacatggc aataacataa tatacatgct
     2341 atcatgacgc tgtaccccta gtttatgtaa aaacattccc tcacataaca taagtaagaa
     2401 tgatataaga aatccaatca agctattttt agttgacata taattagatt tattagatat
     2461 tattccacca agtatgaaaa atacaggtgc aaaaaatata ccatttcgtg tataatctga
     2521 tacttcaaaa atatacccat ataaattctt taatagtggt attttttcta aaaaaccata
     2581 ataactatca ccaaacatac caattacata aataacaata gtgattacta gtgagtactt
     2641 taaacctagt ctttttacaa gaaagtatgc aatacttgca ccaataattg atgcaggtag
     2701 ataccataaa tgatataaag tcccatcaaa tacaatgtcc ttaataatat tgggcactaa
     2761 attattcatg gaaaaatatc cagtgtaaat atttaaggga atataaatta caatagctat
     2821 cccgtaaata aataatacat tttttagaaa agatttcagt ttattacaat tataattata
     2881 tgttgaaatt agaaaaaagc ctgatagcat aaaaaagaat ggcacagcaa tacgagcaat
     2941 tatactagtt aacacaaagt cagccatttt actatatgat aataatggag atgtatgaat
     3001 tgcaattata agtaatgcag ctataatcct aaaataatca agccctgcat aagatttatt
     3061 cctaatcatt cttgttcctc catttcttag tcctttctat ttctccaaaa tgtcacaata
     3121 aaaccatcct tattgttacc cgatatttga tatacacatt gttcaggtat agagattctc
     3181 acttcttctt tagtatgaat aggcacataa aagatttcta taattttttc tttatcttct
     3241 atttttaaac aatttgtata cgaaaatctc ttaatatatt ctatatattc ttcaagagcg
     3301 agattcattt tattgataat ctcagaatgc ggatatccca cataacgaaa atgccatggt
     3361 tcttgagata ttcctgttat ttcttctttt ttacactgat aacgctctat aaaaccataa
     3421 tgtagtgctg ccttacgaaa ctcattacaa ataccattat aaggaaattc agggcagata
     3481 aaatctataa cttctttctt taaccctaaa tcaatagcaa gccctgtctg atgttcactg
     3541 tgatttggaa gtgctacaaa ctttttagta aagtcttttc cattgtcacg cagagaactt
     3601 gaatatatat cttgttgttc ctctgcagta cgatatccac ttacaggtac tatttcattt
     3661 attccacata tactttttaa aatattttgt aaaatgtttg tagcttcacg tttcataaga
     3721 atacttggaa accttgtatc tacaggaatt aaatctatat cttcagatgt tttaactggt
     3781 aaaaaagcat ttactagaat tagattccca tcataaactt tatctttagt caataagata
     3841 gtatttgtca ttttatcttc tttattttta tctaataaaa tattattcat cattttatgt
     3901 acacctcaat tatcttgtcc aatatttctt caaaagacaa tccaatacct ttcatcatat
     3961 ttggataacg actatgagaa gtaaagccag gaattgtatt gacttcatta aaaataattt
     4021 caccagatgg agttaaaaac atatctactc tagcaaatcc agaacatcca agagttctat
     4081 aaataatttt tgctgtctct tgtattcttt tctcacaatc caaatcaatt ctagctggca
     4141 tatgaatttt tgaacttttc aatgtatatt tttctgtata atcaaaaaat ccttttgaca
     4201 attctatttc atctactctt ccaacaagta agtcatcgtt tcctaaaact gcacacccta
     4261 cctcaaaacc atcaatattt tcctcaatta ttacttcatt atcatgttca aaagccaagt
     4321 ctactgctac ttgtaactct tcttttttat aaattttact aataccaaat gatgaacctg
     4381 cacgcacagg ttttataaat aatggataag ttaagtcttt tgacagttca gccacattaa
     4441 atttatctga gtattttagt aatattgatt ttggtacttt aataccttct gaatgaacaa
     4501 gtttatgtga tttgtctttg tccatacata atgctgaaga aagagtacca catcctataa
     4561 cctgtatacc tgccaattca aataacccct gcactgttcc atcttctcca ttttttccat
     4621 gcagtacagg aaacacaaaa tcaagtttag tgtatttaat atcattttca catgattcaa
     4681 tagcactatt tggaatatga ttctgagaaa cagcatcagg aattaaaaat tcaataatac
     4741 catttttgac acggttctgc gaaacaataa ctggaattat atcagtatcc ctttcaaacc
     4801 aagtgttatt ctggatatta ttataatctc caatataatg ataccaatta ccctcccttg
     4861 taattccaat tggtataata tcatattttt ccgtattcaa attctcaagt actgcaaaag
     4921 ctgattgtaa tgatacatca tattctgttg agtttccacc aaatataact gcaatttttt
     4981 tattattcat ctttgattct cctttgttga tttatttatt tttatacaat ctacagaaac
     5041 atgttaaaat actttttcta tttcaaatcc aatcaacctt cacttacaat tgcatctgtt
     5101 ccacataata tattttcaca tttttctatt gatataatta ctgtatcaaa ataaaagcac
     5161 tcttgttttg atacagtaat tatatcaata acaagagtgc tctttattta tttatcctaa
     5221 agaatttcct atttttttat acttaaattc ttacaaattt cctacaatcg gaataataac
     5281 cttaaagata attatattgt tctcgctatg agctgttatt gtcccgttat gaagctccac
     5341 tatttcttta gctatagcaa gtcccaaccc agctctacca ctgtttgaat tacgagatgt
     5401 atccaacctg taaaattgtt caaaaatacg ctcaagtttt tcctctggaa tcgtattgct
     5461 atgatttata aatttaagct ttatataatc atcctcctgt acagctgtta tttcaatagt
     5521 agtatcttca aaactataat aaacagcatt tcgtaataag ttatcaaata cacgttgcat
     5581 tttattaaca tcacacctta tcatcatatt tggtgtaaca ttcagtgtat attttaaatt
     5641 cttactcatc aaagtaggtt taaactcata tgttagttgt tcaagcatac gagttaagct
     5701 tactgtactg tattcaagtg atatatttga taaattaaac cttgtaattt caaagaattc
     5761 attaattaaa tcttcaagac gctctgcctt atccaacgaa atagataaat atttttttcg
     5821 cagttcttca gaaatctgac tttcatctcg aagtagtgtc agatacccta taactgatgt
     5881 aagtggtgtt tttaaatcat gtgcaagata aacaataagt tcattcttac gttgttctgc
     5941 catttgtgtc tcaaatttac gttgttcaag tatatgttta attgagttta ttttcttttc
     6001 tattgccaat aactctggag ataaagccac ttctccaaca tcttctttga taagagagtc
     6061 aataccttga tttatttcac taaaatattt tgtaaaccaa tttaaataaa cccggaatat
     6121 tataaaaaac acaatcacca tagctagtat catgaagagc tccatgctat tacggaaaat
     6181 ctgctgatat aaattcaatg cagctttata ttccatacga aaagcatttt caaagaatga
     6241 tactatccag atagaaaact taccatacaa tataaaagaa tataaaatcc atatgaaaat
     6301 aatggctata aatgccatta aagctgttct cataaaaatt ttatttctca cttgagtaaa
     6361 gtcaggattg actttaatat ttttactttt caattgtata acctactccc catacagttt
     6421 ttataaattt cgggttttta gaaggttcat gaattttttc acgtaatcgc cctatatgag
     6481 ccataactgt gttattatta tcaagatatt tttctcccca tacagcttca aacaattctt
     6541 cagaaggaac cacttttcct tgatgttcac ataaatacca aagaatagaa aattcaatcg
     6601 gtgttaatgc tacttctttc ccaaataaac tacatttatg tgtttcttta ttgataatca
     6661 atcctttaat atcatactca ttaacaatta tcgactgttg ttcataagaa ttattatatc
     6721 gcatatatct tcttaattgt gtctttactc ttgcaacaac ttccaaaggg ttaaatggct
     6781 ttgttatata atcatcagca cctatggtta aacccattat cttgtctata tcttcaattt
     6841 tagctgttaa cataatgata ggataatagt atttttctcg aattttctgg caaatatgaa
     6901 atccatcaat atcaggaagc ataatatcta aaatagccat atccaaattt ttagattcaa
     6961 tacattctag agcttcctta cctgtataaa acttgtatac tgtaaaacct tcatttttta
     7021 aatatacttc aattaaatct gctatttctt tttcatcatc cacaatcaaa atattttcgt
     7081 tcatagatta tttttcactc tccttattat cgaggtcatc atttataata ttggtaaatt
     7141 tacatatcaa ataattacaa gcatatttgt aactaatttt tataagaaaa gaactactac
     7201 atcagaatgt gttgatgcta ctattggaac acaaaaactg agattaattg ttattataaa
     7261 aactccccta acacgtcctt attatcgcat aacaggtatt aactttcaat aaaaatatct
     7321 atcagtcaaa cacaattcaa tttattatac cagagacaat ccatataaaa aacaattaaa
     7381 cctatgtatg taaccacaga cattactaaa atttccatgt tattttatat aaaaattcaa
     7441 aaaaggagtt gaatttaaaa tttgattaga aaaaaatcaa ttttgagaca actccttttt
     7501 attttaatgt atttaaatta tagtatatca gccttcataa tatcctcaaa atcacctttt
     7561 tcagatatta atcttatcaa tttaacagta cagcttaaat catttataag tatagctcca
     7621 actaatgaac catctttcat aaataatttt ttatatattc tttgaccttc atcttttgat
     7681 gaaatttctt tatcaaaatt ttgtatatta ccaatactaa atacttttac attcattgca
     7741 tctaatgaaa ctggatatac ttcactttca aatgtcaaat tatctccact tgcatttgcc
     7801 cctgctattt ttcccatttc tactgaagat ggccatattg ctaaactaat atttcccact
     7861 tgtgcaacat ctccacaagc atatatatct ttaacatttg tctgtaatgt cttatctaca
     7921 acaatacctc tatcaatttt tacagatgta tcctgaacca tttgagtgtt tgctcttaca
     7981 ccaattgaaa atactaccat atcacagtcg acactatctc catctttaaa tacaactttt
     8041 tgagcttttc catcaccttc aataccatca actgctacac ccaatcttac atctattcct
     8101 gtatctctta cacattgttc taatatttta ctaccttctt tatcaagttg ttttgatagt
     8161 atgctatcca tggcttcaac tactactact tctaatcctt ttagtctaaa ttcccatgca
     8221 gcttcaagtc ccaataaacc gccaccaatc actacaactt tcttagattt ttctagtgct
     8281 tcttttacac tatataaatc ttttatattt ctaagagtaa acacattttc taaatcatgg
     8341 cccttaattg gtggtataaa gtttctactt cctgtagcta ttacaagctt atcaaattta
     8401 atagctccat cattcactat aatagtttta ttcacttcat ctaatttatc tacattcgtt
     8461 cctaatatta ctactatatt atttttatca taccaatctt tatcttccat gtagaaatct
     8521 gaaccaagtt cttcatttat accatcagaa agtgcaggtc tatagtaagt caactcatct
     8581 tcattagaaa tcatagttat cttacaagtt ttatttcttt ttcttatagc atctgctgca
     8641 taaaaaccag ctgctccatt tcctactatt acgaaatatt catcagtatc ttttctaaaa
     8701 gtaacttcat cttcttcaac ttctataaat tggtcttcct tagctccaca tacagggcat
     8761 acactaggag cactatcacc ttcaaaaact tcattacaaa ttacacattt aaatttctta
     8821 gttttctttt tacttccaaa agtagaattt aacttatcta ggaatttacc tgtataagaa
     8881 tataatttat caatctcttc atttgatggt ttaaagttaa cagcaaaagg ttttactgct
     8941 gtcattctaa gctgacttat tctctccata gcattttcta taccttctcc actccatcca
     9001 tatgaaccaa atactgaagc aacttttcca ccatgaagaa ctgggtttaa cgatattaat
     9061 atatcccata taggctttaa cgcatctcct aatattgttg gtgttccaaa taatacacct
     9121 tgtgatactg ctatttttgc caaaatatct tcttgcttat gctctattac atcaaataaa
     9181 tttactttat agttgctatt tttttcaata tatgctttta tagcttctgc cataatcttt
     9241 gtatatccat gtgcagaaac ataacaaatt gtaacttctt tttctaactt tatttgttca
     9301 tttacactcc agttgtaata aaggtctata atttttctag gattttcagt taatactgga
     9361 ccatgacctg gacatacagt atctatatct aaatctttta tcttttctat agcagttacc
     9421 atagatggtt taaatggacc cattatacaa tcataatagt atcttaaagc atccaagtaa
     9481 tccttttctt cattttcatc aagtgtattt actatcttat cattactata gtgactacca
     9541 aatgaatcac atgttacaag agttttatcc tcttttatat atgtgtatat ggtatctggc
     9601 caatgaagca taggtacaga gaaaaattct aaagttttgt ccccaattga taatgtatct
     9661 ccatctgtaa ctgcaacata ttcaaagtct ttgtttacga tttccttaag atatttaatt
     9721 gctgtttctg aagctactac ttttgcattt ttagctaaat ccaaaagctt ttcaacgctt
     9781 cctgcatggt ctggttcagt atgacttaca acaatatagt ctattttttc aaaatctata
     9841 tttaaatcat ttaatctttc aatataattg tcaaaatgct tatctttaac tgtctcaaat
     9901 aaaactgttt tttctgttcc ttttaataca tatgaattat aagtagttcc ataaggtgta
     9961 tacataatta tgtcaaatac tcttaaatca gggtcaagcg aacctaccca atataaattt
    10021 tcttttattt ttagtgattt caatgacata ccgtactcct cctaaaatag ttatttagtc
    10081 tctttatacc ctgcttgtta tattttaaac aaaatttatt caaaaaagaa attattttaa
    10141 agatagttta aatcttctaa tcttcttttt ctaccttcaa tacagttccc tttttagaat
    10201 ctacttttat gtcatattca tttttatctt tatctattct tattttataa aaagtaacat
    10261 ctaaatcttt gtctaaactc cattctttaa ctattccatt ttgttctttt aatgcaattt
    10321 ccatagcttg ttgaggtgtc attatgtcat ttaaatctaa tttttctttt ctagcaactc
    10381 catttaaatc ttctgaatcc aacttttctg ttttatcttc taatacatct tttgtattag
    10441 catcaatttt catcttatat tcattattat catctacacc ttccacagta taaacaaatt
    10501 tatttaaatc tctctcaaga cttaaatcta ctatatcagc atccttatat ttatctttaa
    10561 atatgtttat tgcatctgtg tatggcactt taaaatcttg ttccattaaa ttttcattat
    10621 ttttttcatc ttttaaagcg ttatttgatg catttgcctc cttttttgta ttatcttgtg
    10681 cttgtcctga atccttagtt ggactcccac tacatgcagt taataaaccc gctataagca
    10741 tacttaccac taataaatac gtttttttac acataaaaac cctccttaaa tatattaatt
    10801 tattctatat tatgccctct aaacccttta tttacccttt tatattaaaa attcctccta
    10861 ttttatcaat tttaaaactt tatatttact aaaattaaag ttacataaaa attatactgt
    10921 ctcaaaatgg aaacaagcct ctttttcatt gttgaaataa taaaaatata ttttatttcg
    10981 tacaaaaaga gtgcctcatg aactaaaaag ttcattttga gacactccca tttttttata
    11041 tttttactta caaaataaca gtacttatta attttttaca aaccttataa gtatatctat
    11101 atgatattct taaatttaaa cactatatta aaaaattaaa ttttgtttaa ttcttttacc
    11161 gttaaatcca ttttaggata tggaatcgta attccttctt cgtcaaacct aactttaacc
    11221 tcttctagta aatcaagata aattgtccaa taatcttctg tcttgcacca aactctagtt
    11281 gcaaatttaa tagcattatc tccatgttct actactccta taaatggttc tggttcattt
    11341 attattaatt tatgattatt aacaatatct ttaagtactc ttctaacatg tagtatatca
    11401 tcttcatacc ctacactaaa agtcaaatca actcgtctag tattctttgc tgaatagttt
    11461 attagactat catttgatac agaaccatta ggtataagta tctgtttatt atctggagta
    11521 accaattgtg tataaaaaag acctatttgt tcaaccgttc ctccatgacc tgctgcttct
    11581 acgtaatctc ctactttaaa aggtctaatc aatagtataa taaatcctcc agcaaaattt
    11641 gatagactac cttgtaaagc caaacctatt gctacccctg ctgatgctat cacagcagca
    11701 aaactggaca aactcattcc ccaatattcc agaacaatga ttataacggc aactttaagt
    11761 acactcaaaa gaagagactt taaaaacttt cttaaagtta aatcaatatc tcttttacta
    11821 agtaataaca taacatggtt tacaaatttt tttattattt taaatccaat agaaagtata
    11881 agtatgccta ttataagttt tactccgctt gtagtagccc attctagcag actttccatt
    11941 atacctccca tatccatctt attaatatct aaattattta aatctaactt gcttatatca
    12001 tctattttcg tcgtatccat aaaaaactcc ttaccttata ttttagaacc aatattatag
    12061 tttaaagttc ttattttcta tttaatataa tacaatacca tttgtacata gtaaacaaga
    12121 tttatattcc aattatacat attaggaaaa tgcaaactat ttgttatata tatgcaaaat
    12181 ataatactaa attaaactat taataaaaca ctatatagcc ttaaaatcat cattttttac
    12241 ccctatatga gatgatacta tagttataat acttagtact ataaatacta caaaaccttt
    12301 tttatatcca tcaactatat tatatgaatt tacttgtaaa gagatagcta taatcaaatt
    12361 aataattcca cttcctataa attcaaaagt attaataaca ctagttgcca gtccagaata
    12421 aatttcttta tttttatact tggcatcatt aaaaacctgt agatgtgaca taactataca
    12481 tcccataata aagaatgata ttggtaaaac tataattggt ggttttacct ggtatacaac
    12541 tacaattaca atccatataa acaaatctat cattgctcca aattttatta tattaaattt
    12601 gctagacctt attttagcaa atacaaaatc cataattatt gaaccaaata taaatccata
    12661 tgtaaaaaat gatactataa acgcagaaaa actcttactc acaccataaa cgtccattat
    12721 gtatcttact ccccacaagc ttataaatgc acttgtaaga ccaacaaaag agaacattat
    12781 cattgaatta taccatgttg atttgttctt tataacagac ttaataccat caacaatatt
    12841 aaccttctct gacttttcat agggttcagt atctacattg aatccatact cttttggtgt
    12901 atctctaaca ataatataca taaaacatcc aattacaaca ccaattatac cgattaataa
    12961 aaatgaattt ctccaaccaa caagttcaga caaaaacaca agtggaaaag tagcaagaac
    13021 tccacctaaa tttcctacaa atgaaaattt agctgttgca gaagcaaatt ctgacttatt
    13081 aaaccatctc ccttgaattt ttaaaataca aagtaatatt actgatgttc ctgcccctac
    13141 aattacacga gaaatatatg ctaattctat actttgaatc aaaccaaata aaattgaccc
    13201 taaacttgcc attattattc caagactgct aatttttctt gctccatatt tatcaatcaa
    13261 aattcctgca ggtatttgca taaatgcata tgcatatagg caaaaggatg ctatatttga
    13321 aatttgtata gaattaaaac ctaactcatt tgctaaattg tctgaaacaa cagcagttga
    13381 catccttaaa aataatacta aaacaaatga aaatgtaatt ataccccaaa cactccactt
    13441 agcctttgta taacttttca tatcttctcc ctcaaatcca tatattttta aattttcttg
    13501 atataactta tataaagtgt atagttattc ttacaaattt ttacacgcaa tatcattata
    13561 caacatatgt atatatttaa ctagaaataa aacactattc atattatata catgaattaa
    13621 ctacataaat aataagagcc atcttttaac attactcaaa agatagctcc atataaaaac
    13681 atttattcta aatttaattt tttatctaat ataaattttg tagcgaaaaa taacgctatt
    13741 gcaactacaa gactaccaag tattgcaagc ataaaactag gactctgata aagatgatat
    13801 accatatttg tatcttcatt tggtaagatg cttccaactg catctccaac aattgatatt
    13861 actatattta tagctataaa agaagcaaat gccactatgt ttctatgttt attgaatttt
    13921 ggcaattgtc ccattgagat tgatgtatat aataacaaaa taaatgatga atatgaaata
    13981 aacattacta ctactaataa taaaatatct aaaaagtttt cagaaatcca tttaaaacta
    14041 gtgttaaaca aatctaaaat ttcaggtagt aaaattccag aagtaccaaa aagcataaga
    14101 actccaaaag tgaatactgc aactataaaa ctaagtacag cgtatataag tgctgttata
    14161 catttagata atataagtga actagtgcta acaggaagag taaacattaa ataaccttca
    14221 tcccccaaaa gattttttct aaacctttga attgtaacta caatagttaa aactcctaat
    14281 gccataaaaa gacttgttaa tactagtatt cctattccct gtacctgaag tatatttgta
    14341 ttcataaata ttccattaaa tacagctact atcaatatgg ctatatataa cggtataaat
    14401 attcttccac ttgcttttaa ctcatatttt aataatttac ctaacattta aattcctccc
    14461 taaataatgc atctatagat tttccttttt cttctcttat tgcttcaacg tctccttgta
    14521 acactatttc tccttttgat atgaatatta tctcatcaca tatgttttct atttcactta
    14581 ttaagtgggt agcaatcagt aaagtactat cttcagaata attttttaat atagtcttaa
    14641 gtatatatga ccttgcagct gggtcaacac cacctatagg ttcatccaat atatatatgc
    14701 tcgcatttct tgacattact aaaataagtt gaactttttc tttagttcct ttagacattg
    14761 tctttagctt ttcatttaca tcaatatcta agcttttaat catctcattt gctcttctaa
    14821 catcaaaatc actataaaaa tcatagaaaa acttaagtaa atctgatacc ttcatccaat
    14881 catttaaata agttctttct ggtaaatatg aaactatttt tttagtatct atggatggtt
    14941 tcattccatt tatcataatt tcaccattat caggctgtaa taatccattc attagcttaa
    15001 tcattgtact tttgccactt ccattaggtc ctaacagccc gactattttt ccctttggta
    15061 tatttaaatc aatatttttt aaaacattct tagttccata actcttgttt acacctttaa
    15121 attcaactat tccatcttga gaaacattgc ttatattttt atccatttta taattctcct
    15181 ttcagatttt ctgtaataag ttttagcatt tcatctctat catatccaag cttttctaag
    15241 gttactttta atgtatttat ctccacatta gctatttcct ctttcatagc ttttattttt
    15301 tccttatcat ctgttacaaa tcttccttta gttctctgac tataaactaa accttgtctt
    15361 tcaagttctg ttagagcctt ttgcattgta tttggattaa cttccgcatc ttctgctagt
    15421 gctctcactg tttctaattt agatcctatt ttaatttccc ctgaaattat ttttaacttt
    15481 aaatgttcta ctaattgtat atatataggt ttgttattat caagttccca ttccatgttc
    15541 atgcctcctt attatattat tgtattaagt tgttaataca ataatataat aatacaaagt
    15601 atatgtcaat tactttttag taaaagtata aaaaatttta caaaaaaaat gaacactaca
    15661 ttagagtcca tttttaacac aatttattaa attctttata ttacagatta attaccctct
    15721 taccaagtta gatacaatta aatgttcaaa ttcttcaatt ggcattggct ttccaaacaa
    15781 atatccttgt gccatatcac actttacttc ttttaaaagt ttaaactgtt cttctgtctc
    15841 aactccttct gatatgactt tcatacccaa ctcttttgcc atttctatta ctttagtaac
    15901 aattattttg cttcttttac tattataagt ttccataata aatttttggt caagttttaa
    15961 gatatccata ggaatttctt ttaacatatt taatgatgag taccctgaac caaaatcatc
    16021 catagatatt aaaaatccta ttttttttaa attattcatt atatcaatta aaatatcaaa
    16081 attatcaaat atgatactct ccgttaattc aagttctata tactttgcag gtatattgta
    16141 tttatcaatt atatttttgt atgtttctat aaaattattg caatataaat gtatccttga
    16201 aacattaact gaaacagtaa ctggatttat tcctgcatca atccattttc taaggtatat
    16261 acatacttct tcaagcacat ataaatctaa atttataaca aaaccgtttc tttcaaaaaa
    16321 tgggataaat tcatttggag gtattagccc tttatcagga cgtttccacc taacaagtgc
    16381 ttctgaacct tgtatctctc ctgtattaag ttctatctta ggttgtaagt atataataaa
    16441 ttctttattt tctagacttg aaaacattgt attttccatc tctttttctt ttatctcttg
    16501 gtcatgaatc ttattatcat aaaatgcata tgaactttta tgtcctccct ttgccatttt
    16561 atgaggtata ttagccctat ctatgattga gttgatatct ctatcttctg gaagtataaa
    16621 atagattcca caacttaaaa ctaacttata tttttcttct tgattattat taaacaagtc
    16681 taattcttca aatatagttt ttaacctctt tgttatatct tcttcttcta tatatttcat
    16741 aattataata aaattatccg cagatattct tgcaaacatt tcctcttcaa atatattgct
    16801 cttaataatc tttgttaaat tacgcaaaat ttcattccca acttcatatc caaataaatc
    16861 atttatatat ttaaatttat caatatctat atataataaa gcatacttta cttgagtatt
    16921 tttacatagt attttgttag catcaattac aaatttatct atagtatttg ctcctgtaag
    16981 attatctgta tatgctatcc tttgcaattt aattttaaaa tttaatttat catatattat
    17041 atagaatata agtattacac atattatagc cataataagt attatttgta ttgcatgttt
    17101 gctaataagc tgattattag acacaataga ctcaaagtta tttatggagt tgtttttata
    17161 gagtttgcat aaagaataga ctggtgttgt gtatgtaatt tgtaaaatta tacaaataaa
    17221 taaaaaaaac atcaattttt tttgtaaatt cctcataaaa atttcctccc attaaaacac
    17281 ctctatttta ttagcaaata agtacatctc aaaagtttcc atttcttgta aaatggatag
    17341 ttttgaaaag aaaatattaa aattataata taaaaataaa atttttcaga caatttaaac
    17401 aaaaataatc accttttaat aaataattat atgccaaaat aatccaattt attttctatt
    17461 atacctatat gaaatatata aatcaatagt taagacaatg aatatgctac attctataat
    17521 tatagtagca gaaaatttta caaaattaaa gtagatataa actatccatt ttacaagaaa
    17581 tgactacttt ttattaaaat acagtatttg caacatattc acactttatt ttttcatttt
    17641 tttcttatta caatggtttc gacaaatcag taa
//
LOCUS       DAGSWI010000085        17533 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.085, whole genome shotgun sequence.
ACCESSION   DAGSWI010000085 DAGSWI010000000
VERSION     DAGSWI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.085"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            151..1068
                     /locus_tag="MJF80_002545"
     CDS             151..1068
                     /locus_tag="MJF80_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBZ0281199.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            1146..1883
                     /gene="cas6"
                     /locus_tag="MJF80_002546"
     CDS             1146..1883
                     /gene="cas6"
                     /locus_tag="MJF80_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBZ0281200.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            1889..3766
                     /gene="cas8a1"
                     /locus_tag="MJF80_002547"
     CDS             1889..3766
                     /gene="cas8a1"
                     /locus_tag="MJF80_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBZ0281201.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNMIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIDKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKEYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKVQKSESSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFTDKYANKKIQSIYDYKLK
                     SNVLDLLLKNRDLKHITNNILRNKVKNDMESNNKSNISGIDCFRVVQLRVLINSYKKG
                     VYKMDSKNLEKNDEKLKIIYYLGCDIHDYFVNKNSKNKIDGVSYKLLNSVKVGNKSDF
                     MDTIIRVFMSAEKQIPAFILDIEIEKDLDFESIGHAFISGLISGKYEGKDKLPNEKEE
                     K"
     gene            3768..4682
                     /gene="cas7i"
                     /locus_tag="MJF80_002548"
     CDS             3768..4682
                     /gene="cas7i"
                     /locus_tag="MJF80_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBZ0281202.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNMYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGFADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGEIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            4684..5385
                     /gene="cas5b"
                     /locus_tag="MJF80_002549"
     CDS             4684..5385
                     /gene="cas5b"
                     /locus_tag="MJF80_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBZ0281203.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            5396..7717
                     /locus_tag="MJF80_002550"
     CDS             5396..7717
                     /locus_tag="MJF80_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBZ0281204.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEIVECNSNMN
                     IGEYTEKHLVKEFGSLREVQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIIGVGEANDTFLGLLHSTAIDYLEESNQENSNEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLADLTCNTEKIRHCVSIVENSIDENLGKII
                     QNGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            7732..8283
                     /gene="cas4"
                     /locus_tag="MJF80_002551"
     CDS             7732..8283
                     /gene="cas4"
                     /locus_tag="MJF80_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBZ0281205.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            8286..9266
                     /gene="cas1b"
                     /locus_tag="MJF80_002552"
     CDS             8286..9266
                     /gene="cas1b"
                     /locus_tag="MJF80_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBZ0281206.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            9269..9535
                     /gene="cas2"
                     /locus_tag="MJF80_002553"
     CDS             9269..9535
                     /gene="cas2"
                     /locus_tag="MJF80_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBZ0281207.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   9719..10076
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9719..9747
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            10266..10694
                     /locus_tag="MJF80_002554"
     CDS             10266..10694
                     /locus_tag="MJF80_002554"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBZ0281208.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKRFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(11148..11927)
                     /locus_tag="MJF80_002555"
     CDS             complement(11148..11927)
                     /locus_tag="MJF80_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281209.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTFYCCGNCDSEISLSQREHFKI"
     gene            12251..12796
                     /locus_tag="MJF80_002556"
     CDS             12251..12796
                     /locus_tag="MJF80_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBZ0281210.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            12828..13964
                     /locus_tag="MJF80_002557"
     CDS             12828..13964
                     /locus_tag="MJF80_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBZ0281211.1"
                     /translation="MEEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            13968..15359
                     /locus_tag="MJF80_002558"
     CDS             13968..15359
                     /locus_tag="MJF80_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBZ0281212.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRIIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDILNIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            15435..16310
                     /gene="dpsA"
                     /locus_tag="MJF80_002559"
     CDS             15435..16310
                     /gene="dpsA"
                     /locus_tag="MJF80_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBZ0281213.1"
                     /translation="MSNQYDITVIGGDLRQMYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     STKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            16313..16903
                     /locus_tag="MJF80_002560"
     CDS             16313..16903
                     /locus_tag="MJF80_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBZ0281214.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(17110..17310)
                     /locus_tag="MJF80_002561"
     CDS             complement(17110..17310)
                     /locus_tag="MJF80_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281215.1"
                     /translation="MKKLKTISIFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
BASE COUNT         7203 a         1683 c         2844 g         5803 t
ORIGIN      
        1 atatggtaaa tgtcaaaatt ataaaaaaat tctacttata taaaatataa agaggttata
       61 tgtacatatg tgtagaaata aataataaaa atatcaatta atgttattag gggggggtta
      121 ctcattagta aattagctaa tacacttaga atgatgatat taattcaaag cagaggcaaa
      181 atgaaatgca aagaattatc agaagagttg gaagtaagtg aaaggcaaat aaaaagttac
      241 aagatgtatt tagagcaagc tggtatattt ataaattcaa cacctgggat ttatggtggt
      301 tatgaaatag ataaatgtaa tagcatctca ttaattaaat tattagatag tgaagtttca
      361 attctagata tgataaattc acagttagag tataataatg atatttacaa aaatgaattt
      421 aataatattg tagaaaaaat aaaagcagta ttaaatacag gagaaaaaag cgatacatat
      481 atggattatt ttacagttca agcacaacgt aattgtgatt atgagtctga aaaaaataag
      541 tgtaatgaaa taattagagc atataccaca aaacacaaat tttggataga atattattca
      601 ttaaattcag gaaatagtga aagaattgtt catccatatg gtttatttaa ttataaatca
      661 gatacttata tggtagcttt ttgtgaaaaa agatttaagt ttatagactt taaattatgt
      721 aggattaaag attataaggt tttagaagaa aaatataatg tagataaaag ttttagttgg
      781 gatgaatata gtaaaaatag tataggaatt tataagggag aagaaattaa tgttgttata
      841 aaaattagtc atccattttc aacaattata aaggagaaag tttgggttaa taatcaacaa
      901 atcatagaat atgatgataa atctataatg tttaaagcta aaatgagagg ttatgaggag
      961 ataaaaagtt ggattttaag tatgggagct tatgttgagg ttgtagaacc agatagactt
     1021 agaaatgata tattgttaga gattgaaaaa atgaaaaaaa tttactgatt ttttcaaata
     1081 atttatactt ggaccaatat acttcacggt ctagtgttaa tatattaatt gagagaggtg
     1141 atattatgaa aatgaaaatt gaatttagta caggttgtat accaatatct tataattcgc
     1201 tctttatgag tattataaaa gaagcaataa gaaaatctaa tgaagattac tataaaaaca
     1261 tgtactatta taaagaaaag aataataaaa aaactaaaaa ttttacattt tcggtttata
     1321 taaaaaagta tagtattgaa ggtgacaatt ttattataga agataaagta atattaaata
     1381 taagtactcc agatttagaa ttgggttttc atatatacaa tgggttaatg acttctaaaa
     1441 aatgtttata taaagactac gaacttacaa gaattagaat cgatttatca agagaaaaga
     1501 aggtaacaga agaaagggta ctatttaatg cattgtctcc aatctgtgta aagtctaaag
     1561 aaggaaaatt cttggagata actgatgata gatacataga agaactaaac tatataacta
     1621 atgaagtggt taaaaattat agggggaatg gattaaaaag aaagttagaa tttgaaaata
     1681 taggtctcaa aaaggtagta gttaaagaga gtttaagaga atttaaaaaa ataacaggta
     1741 aagagtatca atatattaat ggttacaaag gtaaatttat attgaaaggt gatattgatg
     1801 atttaaatct tatctacaat ctaggaattg gttttagacg tgctcaggga tttggagatg
     1861 tagatatttt agaatggagg tgagataaat gaagttaaga gtttatagag gagattggtt
     1921 ttttaatatg ggtatagttg gatttttaaa tataataaag aaagcagata aacaagctga
     1981 aatatttatt atggaagatt atatcgaatt tgatagctta tttcttgaaa actttcatga
     2041 gtattatttt aattacttta tggatgaata tgatgtatca aaaagaatta aaaaaaatat
     2101 tgattacagt ataaatttta ttaaatctaa gcctgataga atcaaagatg gaattaagaa
     2161 aattaaagat agtgtgaaac aacaaaatga taagattaaa aaatttgatg aagaaaaatt
     2221 taacatgata aaagagaagc tagatagtat gtctaagata aaaagttatg atgaatttga
     2281 tagtttagaa gctttagtag atgagactat agatatattt aaaataaaat caataaatga
     2341 caggcttaca gcaaatcttt ataaatatgt agttcaagat aattattttg gacaagttag
     2401 tttttttaat gttgctaagg cgaaattaga tttagatgga ttaaaacagg taatgtttaa
     2461 tgattattta agacaaatta tatattttgg agagttagaa gatttattag aagagaatga
     2521 ctatgataag ttgaaaaact atttaaatga tagattaaat agtatagata aggatataaa
     2581 tgaaaaaaaa gttagtaagt ctagtataaa tactatagaa aaaattatga aggaaataaa
     2641 taaaaacttt ataaagaaaa ataaaagcat agaagatata aaagagtata tggatagttt
     2701 agaagtatgt gaaatgtgtg gtttatataa gggaatatta gatgaatact cagaaagtaa
     2761 ttttgctcca cttggagtaa gcaccaataa tgctaggaat atgttttgga atcaggcgta
     2821 tgtaccatca atatgtgata tttgtaagct aatattgttc tgtactccag caggggctac
     2881 ttatacaaga aagaattatt taattaatga agaaaatgag ttttatttat ttgttaacat
     2941 ggatacttgt ataaatgagc tttttgaaag aaataatagt ttaaaagttc aaaaaagtga
     3001 gtcttcagat tccaaagatg aaaatccttt taatcagctt ataaaaagta tagtagaaga
     3061 aaatacatta aaaagtgaat ggcaacttcg taatatactt tttgtggaat ttaaagcaag
     3121 tatagattct aaaaaatgta aaataaatta ttttaatatt ccaacttatt tagcaaagtt
     3181 ttttacagac aagtatgcaa ataaaaaaat acaaagtatc tatgattaca agcttaaatc
     3241 aaatgtacta gatttactct tgaaaaatag ggacttgaag catataacga ataacatttt
     3301 aagaaataaa gttaaaaatg atatggaaag caacaacaaa tcaaatatat caggaataga
     3361 ttgttttaga gtagtccaat taagagtatt aataaatagc tacaaaaagg gggtatataa
     3421 aatggatagt aaaaatttgg agaagaatga tgaaaagctg aaaataattt attatttagg
     3481 gtgtgatata catgactatt ttgttaataa aaattctaaa aataaaatag atggagtaag
     3541 ctataaactt ttaaattcag ttaaagtcgg aaataaaagt gattttatgg atacaattat
     3601 aagagttttt atgtcagctg aaaaacagat tccagcattt attttagata tagaaattga
     3661 aaaagattta gactttgaat ctataggtca tgcttttata tctggattaa tttctggaaa
     3721 atatgaagga aaagataaac taccaaacga aaaggaggaa aaataagatg aaaagagggt
     3781 taacatttac agtaatagca caagctcaaa gtttaaacta tggtgaaggg ataggtaata
     3841 tatcagaatt aaaaaaatta agtagagatg atggaaatat gtatacattt gcatctcgcc
     3901 agtgtttaag atatgacata gtacgattag cagcagaatt gtttaattgg aatttacagg
     3961 ttgtcgataa agaaaaaggt actattcaat ttaaagataa tataagtata agagaatcag
     4021 aagaaatgga tttatttgga tatatgaaaa caaataaaaa agatgagaaa gataaaaaag
     4081 gaggttcttt gactagagaa gctactgtaa gattaagtaa tgcgatttct cttgagccat
     4141 atagaagtga tatggatttt ttaaataata aaggatttgc agatagaata ggagaacacc
     4201 ctaatcttgc taacatagag caacatttaa gttactatac atatacagtg actatagatt
     4261 tatctaaaat aggcaaggat ggagagatag aacttgataa taaagagaaa tgtagaagag
     4321 tagtagaatt tttagaaata ataaaagtat tgaatagaaa tattagaggt agacaagaaa
     4381 atttatctcc tctatttgta gtaggaggag tatatgaaat agcaaatcca tttttcttgg
     4441 gtagaattaa attaaaagga gataaaaatg gatttaaaat aaataagcaa gctatagaag
     4501 aagtaattca aggtactttt ttaggaaaag atttaaaaga atttacctat gttggaatgg
     4561 tagatggggt atttataaat aaagaagaat ttaagggatt gtttgaagat aattttttaa
     4621 gtgtagataa attttttgga caactagtta aagaagtaaa ggaatattat ggggtgaatt
     4681 aatatgaagg ttttgaaatt agatttattt caagaaactg catgctacaa aaaaccattt
     4741 gctatgaaga ttacagagac atatccactg cctccatatt ctacaataaa tggattaatt
     4801 cacaaaatat taaatgctac agaatatata ccatttaata taagtgtaca gggaacatat
     4861 gaaagtatat ttaataatta ccagacaaca tatttttata aaaaagatag tattacatct
     4921 atgccaatga atagtcatat gctattgaat gtaaatttga taattcatat aggtggggat
     4981 tttgatttat tggaagaaat atatgatagt ttattaaatt atgacgagca tttatcatta
     5041 ggtagaaaag aagatttagt aagaattaat gatattagat ttgtagaagt caataaattt
     5101 gaagtagatg aggatataca attagatgat tatgaaaatt ataagttatc agaatgtaat
     5161 ataagaatgc ctatatatat accaaaaaca agtttacaag atacagatat agatggtata
     5221 agttacagac tcaataatta ctatgataat aatgctgtgg aagataaaaa aagggtttgg
     5281 aataaggtag atagttatta tgttgaggat ggaaatacta tatcaattgg agatatatta
     5341 ttagataatg aaggagattt attatatttt aataatataa attaggaggg agattatgtt
     5401 atatgctaaa tctaatccag tggaaacact tagagaacat actgatgaac ttttaaaaca
     5461 aatgaatgta ttaagagaaa gttatggaaa aaatataaat agcttagatt ttctagaaga
     5521 ggagatattt tggcaattac ttgactttgt tatagaattt catgatattg gaaaagcatt
     5581 ttcaccgttt caagagttaa taaaaagcag aatggatact aaaataaatg aacctaaaat
     5641 agtaactcat ttagaaaata atgtaggcca taattatctt tctcctgcat ttattgatta
     5701 tagttatata gacaggaaaa aaaataaaga acttagagca gtattaaatc aagttattgt
     5761 atatcatcat gaaagagata tctttataga taaagatttt aaaaatttaa tacaaaaaat
     5821 attagatgaa gatttaataa ataaagttta tgaactacag catgaattta aagttagata
     5881 tccaataaaa acagaaaaat tgagtaaagt atatctacaa agtgtagaaa aaagaataga
     5941 taaaaatcac aagtactata atttatatat tatgttaaaa ggaatactac atagattaga
     6001 ccacagtgct tctgcccatg agattgtaga gtgcaatagt aatatgaata taggcgaata
     6061 cacagaaaaa catttagtaa aagagtttgg tagtttaaga gaagttcagt catttgctaa
     6121 atcgaataga aacaagaata taatactaat tgcatctaca gggatgggaa aaacagaaac
     6181 tgctttgatt tggatagata aggataaagc attttttaca ctaccattaa gagtaagtat
     6241 aaatgcgtta tttgataggg ctaaaaatat aattggagta ggagaagcaa atgatacatt
     6301 cttaggtctg ttacattcaa cagcaataga ttatttagaa gaaagtaatc aggaaaattc
     6361 aaatgagatt gtagatttag caaagttatt atcttgtaaa ctgacatttt ccacaataga
     6421 ccaaatattt aaatttcctt ttttgtatag aggctatgaa aaagtgtact caacattagc
     6481 atattcaaaa gtagtaatag atgagataca agcatattca ccagaaatag cagctgtatt
     6541 ggtaaaaggt atagaaatga tacataagat aggtggcaga tttatgataa tgacagctac
     6601 tatgccaacc atatatatag atgaattaaa aaaaagaggt gtaatgaata gtaacttagc
     6661 tgacttgaca tgtaacacag aaaaaataag acattgtgtg tctattgtag aaaattcaat
     6721 agatgaaaac ttaggtaaaa taatacaaaa tggaatgaat aaaaaagttc ttgtaatagt
     6781 aaatacagtt aaaagtgctg tagaaaaata tgagttaata gaagaaatag ttaagccaaa
     6841 aggaatagat gtcaacttaa atcttttaca ttctatgtat atacaagaag atagagcaaa
     6901 attagaaaaa tatataaaag aatttgcaga tagtgatagt aatggtattt ggattactac
     6961 acaattagtg gaagcatctc ttgatattga ttttgatgag cttcatacag aaaattcaac
     7021 acttgacagt ctatttcaac gatttggtag atgttataga agtcgtgagt atgaagaaaa
     7081 tagcccaaat atatttatat atacagaaaa agctacagga ataggtagta tttatgataa
     7141 ggatattgta gaaaaaggac ttgagctact gaaaactttt ataaatggaa aagaatgtgt
     7201 gaagatgaaa gaaaaatata aggtagaaat ggttaagata ctatactcta aagaaagtct
     7261 agaaggtaca gcttttgaaa agagatttac cagtgcttta aatattttag atactataac
     7321 gccatatagt ttaggttcta aagatgcaca aaatatactt agaaatattg aaggatatac
     7381 agtaattcca gaggatatat ataataaaat tgaagaaact ttaatcaaag attatgaaga
     7441 gttgggacaa gagcttataa atgcatatag taatgatgat agacaattga taaataaaat
     7501 aaagtctaag agaaaaaaag caagaagaga gataattaaa aaaactgtaa atttaccaat
     7561 atataaagct aaacaaaatg taattgacat acaggtgaaa ggtcttgaag atttaaaaat
     7621 acttttatat aaatatgata tatacgaaaa taagcataca aaacagatat ttggtaaagg
     7681 tgtgcttata agtaatgaaa tagaccaatt tatttgattt ttatattaac aatgtggcta
     7741 aaatttacta aagaaggtga ttttgtgcca atagatttag aagtattaaa agttcaaggt
     7801 gtaaaaataa attattatta tgtatgtaaa agaaaattat ggctattttc taaaggaata
     7861 accatggaac aaaatagtag tagagtattg tctgggaaga tagtacatga agattcatat
     7921 aaaagaatga aaaataaaga aatattgatt gatgatatct tgaagctaga tataataaag
     7981 gacgagtatg taaaagaagt aaaaataagt tcaaagatga aagaagcaga tgaaatgcag
     8041 ttatactatt acttatacta tttaaagaat agtatgggta tagagaaaaa aggaactata
     8101 aattatgtta aggaaaagaa acaagaagaa ttgatattga cagaagaaaa agaaagaatt
     8161 attgaaaaca cacttataaa tataaatgaa ataaatatgc agttatctcc tcccaaaatt
     8221 gagaaaaaac attattgtac aaaatgtgct tattatgaat tttgttttgt gaaggaggag
     8281 taacaatgta taaagactat tatattataa gtaatgggga tataaaaagg aaagaaaata
     8341 cgatttacta ttacaattta gaaggtgaga aaaaaatatt accagtagaa caagttgatg
     8401 atattcatat atatggagaa gtagatttaa acacaaaact tataaattac ataagtaaat
     8461 atggggtgat gcttcatttc tacaattact atggatatta ttcaggtaca tattatccaa
     8521 ggaagaaaaa tgtatcagga ttcagtttag tatgtcaatc agcctgctat ttagataaaa
     8581 atgaaagatt atacttagca aaatcctttg tggaaagtgc atcataccat attttaagaa
     8641 atatgagata ttacaaagtt gatgaaagtt taattgatac tataaaggat gaactaaatg
     8701 atttgaatga tgtcaaagaa atatcagaac ttatgggtaa agaaggcaga ataagaaaaa
     8761 catactataa atcatttaat caaatattaa aagatgattt tgaattttat aaaagagaaa
     8821 aaagacctcc caaagaccca gtaaatgctc ttatgtcttt tggaaatagc ataatgtata
     8881 cgaatgtttt aagtgagata tataagacac aacttgaccc tacaataagc tttttacatg
     8941 aaccatcaac aaagagattt tctttaagtt tagatattgc agaaattttt aagccaatta
     9001 taattgacac aataattttt agcctgatta ataataaaag aattaataag aatgattttg
     9061 aatatcaaga caaaatttgt tatttaagtg aaaaaggaaa gaagaaattt ttgcaagaat
     9121 ttgaaaataa gatgcagaca actataaaac atagaaatct aaatagaaaa gtttcatata
     9181 gaatgtttat aaggttagag tgttataaat taattaaaca ttttataaat gatgaaaggt
     9241 acaaagtttt aaaggtgtgg tggtaagtat gtttgttatt gttacttatg atattgttga
     9301 agcaaggtcg ttaaatagaa ttagaaggat acttagaaaa tatttgactt ggacgcaaaa
     9361 ttctgttttt gaaggcaata ttactgaagg aaagttacat aaatgtattt ctgaaataga
     9421 aaatattatt gataatagcg aggattcaat ctatgtttat gagataaaaa atcctaattc
     9481 aattaaaaag aaatgttatg ggattgataa gtattctgat gaaatgttta tataggtttg
     9541 cagtgagcga tatttatgct aaaataggtg ttaacagttg gaatataagg gattgaaggt
     9601 gtatgataac tgttatcaat tgcactactg ctcgctcact gcaaattttg atgtttttat
     9661 tgaattataa ttgcttgatt gaagtgtttt caatgtattc aaatatacct attttggggt
     9721 tttatattaa ctaagtggta tgtaaatttt ttcattttta tttttttagt tgcattttca
     9781 actggtttta tattaactaa gtggtatgta aattattttt tagctttttc tctatatgta
     9841 ccaatatcac gttttatatt aactaagtgg tatgtaaatt agaaatacaa ttcggaactt
     9901 gtttctgttg aatttgtttt atattaacta agtggtatgt aaataggttt aaaaacctct
     9961 gttcttccac cataataccc agttttatat taactaagtg gtatgtaaat aggataaaga
    10021 aaagactcac acaagacaca gtgtcaggtt ttatatcaac taagtggtag ataagtagaa
    10081 tattagcaat atcaacgagt atttagaaac ttattttata ttaataaagc agtatataaa
    10141 attgtagaat caacaatagc atatactaaa acatccacct tatattaact aaatcatatc
    10201 taaataaaat acacttacct acaaacatta taaaatcaat acaaaaatga ggtgaaacaa
    10261 aatttatgat aaagaaatta aacagtaaag atataaataa aatcatggaa atatgggaaa
    10321 aaagtacaat caaagcacat gactttataa gtaaagaata ctggcaaaat aactacaata
    10381 ctgttaaaaa cgaatatata cctatatcag atacatttgt atatgatgat ggagatgaaa
    10441 taaaaggatt tataagcata atagataaaa gatttatagg agctttattt ataaagccca
    10501 aataccaaaa tctaggtatc ggaggtaaac ttttagatta tgcaactaaa aaatataaaa
    10561 gtctaagctt agcagtatat aaagataata aaaaagcagt tgtgttttat aataaaaaag
    10621 gttttaatat agtaaaagaa caagtaaatg aagattcagg atttaaagag tacataatgg
    10681 aatatagtaa ataatatgat tacattctat taaaaggata attaatttat ttgccatttg
    10741 attttttaag agtaattttt aaaatagact atatgaagat aaagatattt attatagatg
    10801 taagttaatc agaattgata tagctacaaa agaggaaaat aaaattgtat ggtgtatttc
    10861 agatttaaca aatttactat ttaaagataa taagtttgat ataatattaa agatactttc
    10921 acctataaac tatagagtat tgaaagaatt ttaaataatt atggaatgct aataaaggtt
    10981 tgttaaaatt taataattat tagattatag aaaattgcca tgaaattagt tcatcaagaa
    11041 ctagtttcat agcaatttta attcttataa gtgtatttat ttttacagat ttaaaaaact
    11101 tatacagtat ctaataatgt aatcttatta ctttagccta taaatattta aatcttaaaa
    11161 tgttctcttt gagataagct tatttcagag tcacaatttc cacaacagta aaatgttgag
    11221 acatctttaa atttttcagt attctcatca aagttcattt ttttctttgc aataattcct
    11281 accttagtta aattagaacc acaattatta cagttttgaa ttttgcatag gttatatagt
    11341 ttatcaagtg taatatcttc taataattca tcacaattac aacatctgat agaagaaact
    11401 tcactacaca tttcaatagg ttcagcttct ttagaagaaa atttatatgt tatgtcattt
    11461 gatactttat aacctgtatc aaatatatta gaattacaac ttggacataa gtactttgga
    11521 agatatatat cttttttttc aaaagtatca ctactaatat tacagtttaa ttcatctaaa
    11581 attggtcttt catctaggtt attaccttta tttaagctta gaagataatt ataaattttt
    11641 ttatcttcat tctgttttga tgtatctaac aaatgctcca ttatattagt gtattttttg
    11701 ttttttgaag gcatgtttaa atccagataa ttgttttcaa tagcaaagca tactatattg
    11761 aaatagttat atttaactgc aaaaaataat aacatttcat caaatacttt atctattatt
    11821 ttgtcagaaa tacttatcca aaaatctggt gactctaagt aaactttcct actaagttca
    11881 tctaactctt catctatatt atttatatta ttttcattaa ctttcatatc atcacttcct
    11941 tttaaatatc ttgtgtataa actatttgtt actatttttt atataaaata aaatcttata
    12001 taaaaatata tcatattata atttacaagt atagtctaaa tagagctgtt tttaagcaaa
    12061 gttttataaa tgactattaa aatataaagt aacagtataa agtttactta taaattaaat
    12121 gtaaacctaa ataattataa tagttgacaa aaaaacgtct ttattttata ctaaaacaaa
    12181 gctttaatag aagctttgta tatgtacata taaactaaat gaaataagac atatgaaggg
    12241 aattataaaa atgaaaaata ctaaagtaaa tattcaagat ttaactaaaa tgtcaatatg
    12301 tgtagcatta ttgtgtatat catcttacat atatatacca ttgccattta ctccagcagg
    12361 agtaacagca caaacaataa tgataaattt aatagctttg attttaacac caagacaagc
    12421 atttagtaca gttggtgtat atataatgat aggtttaatt gggattccag tatttggtgg
    12481 aggtacatct ggtattggaa agttgttaag cccaacaggt ggtttttatt ttgggttttt
    12541 gtttgcagca ctaattatta gtttattaaa aggacgaaac aatgatatta agagatatat
    12601 actaataaca atttttgttg gaatgcctat tatatatttt atgggaacag tatttatgtg
    12661 ctattttaat caaatgacag tagaagcagc actttttgca gcagtagtac catttttaat
    12721 tggagataca ataaagtcgg ttattgcatc ttttttaggc actaaactga acaatgtact
    12781 cagaagaaat ctatgataaa tctataatat aaggaagggt gtaatgtatg gaggaagttt
    12841 ttatattagg aggattgaga agtcatatag gtctaaaaaa tggaattttt caatttgttc
    12901 aacctgaatt actaggagca agtgttttaa aagatttaat taaaaagtat gaaattgata
    12961 aaattgatga gattatatgt ggaaatgcag ttgggacagg agggaatata gctagattaa
    13021 tgactttaac agcaggagtg tcaaatgaag tacctgcttt tactgtggat atgcagtgtg
    13081 cttctgctat gatgagtata gacatagctt tttcaaaggt taaatctggt cagtgtgatt
    13141 taattattgc aggtggtttt gaaagtagtt ctctacaacc aatgcgcaca tatcataaga
    13201 atgataagcg atataataca aataatccaa attatacagt tgctcaattt tctcctgatg
    13261 ataatagcca aaatagtatg ctcgaaggtg cggaaagagt agcagagtta tatcaaattg
    13321 agaaagcaga cttggatttt tgggtaaagg aaagtcataa aagagcaaaa gaagcaagag
    13381 aggaaaaaat acttgaagat attatatctc ctataaataa tagtacctat gatgaaggta
    13441 tacgtgataa gatgagtcaa aggttattag ataggatgcc atcaatttta ggaaaagaaa
    13501 ctattacaaa tgcagcaaat tcatgtctta ttaatgatgg agcttcattt attataatat
    13561 gttctaagaa gtatttagaa catgtaaaaa agaagcctaa agcaaaaatt attaatacat
    13621 gcacaatagg aacagatgca accttaagtc caacatcagc gatacaagca atggataaac
    13681 ttttagagat agaaagtctt aattatttag atgtatctgc tgttgaattt aatgaagcat
    13741 ttgcagttat agatgtgttg tttcaaagaa aatatccaga gttaatagat agatataata
    13801 tatttggtgg agctttagcg tatggacatc catatggtgc ttcaggtgca attattgcat
    13861 tacatctttt aaaagcatta gaaaagacta agggtagata tggaatttgc tctatagctg
    13921 cagctggtgg tctaggttca gctttactgc ttgaaagggt gtagattatg aactattatg
    13981 aacttttaag aataaaaaaa gagaggcata aaaataaaaa ttttttaatt atagatggag
    14041 aaaaatatac ttatgaaaat atattgagtt atagtgagga actaggaaaa caaatttctt
    14101 taggtgaaaa tatcccaatt ttaatatatt ctaaaaatat aatgtttcag ttgattagtt
    14161 tttttgctat taattattca aaaaatattc ctattatttg tcattataat ctttctaaaa
    14221 aagtattaga tgatatttta ttaaaaaata atatttcaat cattatatct gatgaatcta
    14281 tggacgttat tgatttatat gatgataaca ataatgaagt agcaaaaaaa gttaattttt
    14341 ctatttttaa tcctaaattt aatatatata tgtatcaata taagaacctt ataaattatt
    14401 tagataaaag tatttgtatg ggagctttat cttcaggttc tacaagtgtt ccaaaggtat
    14461 tatatagaac atatgagagt tgggctggat tttttcctat acaaaatggt atttttaata
    14521 tctctgggga atcaatcttg tttgttaatg gaacacttag ttttacaggg aatctaaatt
    14581 ctataatgtc agtattatat gaaggaggta gtattgtaat aagttcaggt cttaattgta
    14641 aatcatggat aaggataata aaacaatata atgtaactaa catatacctt atacctacaa
    14701 aacttcaact tttggttaaa cacttaaaag aaccagtact caaagttgta agtattttta
    14761 cagggtctca attattattt gaagatactg caagaaatct aaaaaaatat atgccaaagt
    14821 ctgaaattat tttatactat ggggcaagtg aattaaatta tattacatat ttatgctatg
    14881 atgaactaat tgaaaaacct ctaagtgtag gcagaccatt tccaggaata gatatatata
    14941 ttagagaagg aaaaattttt gtgaatacag aatatgcagt atataatgca accaaacctt
    15001 attctgtcaa tgatattgga tattatgata atgatggata cttaatattt gaaggaagaa
    15061 gtgatgacat tttaaatatt ggaggattta aagttagttc tactaagatt gaaaatgaag
    15121 ttaaaaaaat tcctcaaatt gaagatgcaa ttgtattgcc gtactcagac actataagag
    15181 gaaatcaaat tgtattattt gtaataacta tagacaaaat tacgaagaaa gatttgttga
    15241 ttaaaatgaa agataatcta atgaaaaatg aaatcccaaa gaaaattatc tttttaaatt
    15301 catttccata tacctcttct gaaaagatag atagattagc attattaaaa atgttataag
    15361 tataatattt agtaatttta gtgaaccaat gtattgataa aaacatattg tatatagtaa
    15421 aggaggctta caatatgtca aatcaatacg atataactgt tattggggga gacctgcgac
    15481 aaatgtacat ggtaaaaaaa ctaattaata aagggtattc tgtagtcact tatgggatat
    15541 caaatgaaat tataaatgga atcgttggaa aatcaacttc actagctgaa gcactatcca
    15601 aaagttccat tattttatgt ccaattccat ttacaaagaa tcaagttaat attgaaaata
    15661 cggatgattg tcttgatgct actattgata acttgctaat taatttatct cctaatcaaa
    15721 tattatttgg aggtaacata tcaactaaag tttgtaaatt ttgcgagcaa cactctattc
    15781 agatttatga ttttatgaaa atgaatgatg ttgctatttt aaatgcagtt gctacagctg
    15841 aaggggctat agcagaagct attaaaagaa gtataatcaa tatacatcaa agtaactgtt
    15901 tagtattagg ttatggaaga tgtgcacaaa tacttgcaaa aaaattatac gctttggatg
    15961 caaatgtatg tgttggcgca agaaataatg atgcaagaag tacggctaat gcatttggat
    16021 attccagtat ttctttaagt caacttgatg aaaaactttt acaatttgat tatattttta
    16081 acaccatacc agagtgtatt ttaacgaaag aaaggttaga aaaagtatca caagaagtaa
    16141 ctattataga tatagcttct tctcctggag gagtagatta ttctgttgtt aaagaagcag
    16201 ggattaatgc gactctttgc cttgggcttc ctggtaaata ttcaccaaag acatctggtg
    16261 aaattcttgt taacgctata gaaaatatta taaatgaaag aagggattag aaatgagact
    16321 tgaaggactt acaattggag taggatttac tggttcgttt tgtacttatg ataaaatatt
    16381 tatagagtta gagaatttgg ttaaagaagg agctaatgta cacactatat tttcagatgt
    16441 atcacaaaat atcgattgta gatttggaaa ttctgaagag tttatgaaaa aagcatatga
    16501 acttacagga aataaaccta tagttacgat tgaagaggca gagccatttg gacctaaagg
    16561 aattgctgat attataataa tagctccttg tactggaaat actgctgcaa aattagcaaa
    16621 tggtattaca gattcaccag tattaatggc agcaaaaggt catcttcgta atgataaacc
    16681 actagtaata tcaatatcaa cgaatgatgc tctaagcttt aatttcaaaa atattggcat
    16741 tctccttaat tctaaaaata tatattttgt accttttggt caagataatt gcaaagcaaa
    16801 gcctaattct atgattgcac atactgagct aataattcca actatagaac ttgctctaga
    16861 gaacaaacag ctacagcctg ttataaaaag tccacatcaa tagaattggt tcttataata
    16921 ttatgtatta gataaaatac tagcaaagag gctgtctcaa aatagagaga agcctctttt
    16981 tcttgttgaa atgatgaaaa tatatttaat ttcatacaaa aaaatgcctc atggactaaa
    17041 aagtctattt tgagctaatc tccgtaattt ttggtatatt aaaattattt acttaatcca
    17101 atttttaaat tagaatttat atcgttttga ctcttcaaat atcaatctaa ctgttgttga
    17161 tacaaaagaa gaggacataa ttaaaacaaa taccgatagt cctctaataa acaaattatc
    17221 tacataataa gttactattc caatacctcc aatcgttaaa attacactta taatcaaaga
    17281 aaaaatgcta attgttttta attttttcat gcttatcctc cttcttataa acttcaaatt
    17341 tatctatata atattaaata tattttattt catagtatat aaaaatcatg aatatatatt
    17401 tttttaatta tactatatgt tgtaaatgtt tgtacattta actatattta ctttaacttg
    17461 ttaattaagt ttttattttc attttcaagt atttttattt ttaaacttga tagagtatca
    17521 tattaaaaat tct
//
LOCUS       DAGSWI010000086        17156 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.086, whole genome shotgun sequence.
ACCESSION   DAGSWI010000086 DAGSWI010000000
VERSION     DAGSWI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.086"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            573..1568
                     /locus_tag="MJF80_002562"
     CDS             573..1568
                     /locus_tag="MJF80_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0281216.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKTSVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            1546..2409
                     /locus_tag="MJF80_002563"
     CDS             1546..2409
                     /locus_tag="MJF80_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBZ0281217.1"
                     /translation="MSIMSNYNEASSNYSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            2406..3200
                     /locus_tag="MJF80_002564"
     CDS             2406..3200
                     /locus_tag="MJF80_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBZ0281218.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWKQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYII
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLAMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            3485..4864
                     /locus_tag="MJF80_002565"
     CDS             3485..4864
                     /locus_tag="MJF80_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBZ0281219.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVSQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            5312..6025
                     /locus_tag="MJF80_002566"
     CDS             5312..6025
                     /locus_tag="MJF80_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281220.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(6297..7835)
                     /locus_tag="MJF80_002567"
     CDS             complement(6297..7835)
                     /locus_tag="MJF80_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBZ0281221.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            8717..9172
                     /locus_tag="MJF80_002568"
     CDS             8717..9172
                     /locus_tag="MJF80_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBZ0281222.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSSLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            9221..9805
                     /locus_tag="MJF80_002569"
     CDS             9221..9805
                     /locus_tag="MJF80_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBZ0281223.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSCVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(9810..10133)
                     /locus_tag="MJF80_002570"
     CDS             complement(9810..10133)
                     /locus_tag="MJF80_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281224.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            10285..10803
                     /locus_tag="MJF80_002571"
     CDS             10285..10803
                     /locus_tag="MJF80_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBZ0281225.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(10980..11648)
                     /locus_tag="MJF80_002572"
     CDS             complement(10980..11648)
                     /locus_tag="MJF80_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBZ0281226.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            11763..13178
                     /locus_tag="MJF80_002573"
     CDS             11763..13178
                     /locus_tag="MJF80_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBZ0281227.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            13401..13859
                     /locus_tag="MJF80_002574"
     CDS             13401..13859
                     /locus_tag="MJF80_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281228.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            13856..14440
                     /gene="clpP"
                     /locus_tag="MJF80_002575"
     CDS             13856..14440
                     /gene="clpP"
                     /locus_tag="MJF80_002575"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBZ0281229.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            14752..15828
                     /locus_tag="MJF80_002576"
     CDS             14752..15828
                     /locus_tag="MJF80_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBZ0281230.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            15895..>17156
                     /gene="bclA3"
                     /locus_tag="MJF80_002577"
     CDS             15895..>17156
                     /gene="bclA3"
                     /locus_tag="MJF80_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBZ0281231.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGTPGATGPTGATGPTGVGVTGATGATGATGADGATGVTGPTGA
                     TGATGANGLVGPTGATGAAGTPGATGPTGATGPTGVGITGATGATGATGPTGADGATG
                     PTGATGNTGADGVAGPTGATGATGNTGA"
BASE COUNT         6325 a         2062 c         2879 g         5890 t
ORIGIN      
        1 aatattacag taaaatgacc agttttaata tacgtattat atttttaatt tataaacgaa
       61 tattataaat gaaatttaaa ttcaaccttg aagaaataag ctaaatgtat tagtatataa
      121 atccatgctt gaacataaat aacctttatg atatgatatc aataataata ttatgagagg
      181 agtgccttga tgtcggagtt atataatggt aaccaacaat taaatagttg gagacacata
      241 tattctgtat aatcagaaat ttcaagtgta tttttaatac atttaatttt tgtgtcttat
      301 tctgccttta tagataactg acatatgaca acaaatagta tttaaaatat atattacttt
      361 gttaaattat ggtgtagtag gctttaatct gctacacttt tattaatttt gtatagtatt
      421 attttgataa ttattttgaa atagagagca ttaatttttc tagtccacag gagttttctg
      481 tttttgtgct gttaaatata agcacagaaa aaggcagata tatacctgtg gacttttttt
      541 tatataaatt atgaaatatg gaggtagtta aaatgagtat aattgaactt aatgaactga
      601 ctaaatctta tagaagattt gagaaaaagg caggatttat ggggagtgta aagtctttat
      661 ttaataggaa atatacagaa aaaacagcta tatcgtcatt tgatttaaag atagaagaag
      721 gagaatttgt tggattaata ggtcaaaatg gagctggcaa aactactctt attaagatgt
      781 taactggtat tattgcaccg tcttctggag aaatatctgt aatgggttat taccctaaca
      841 aattagaaaa taattttaaa aaacaatatg ctgttgttat gggccaaaaa agtcaactat
      901 tctttgaatt gacaccagca gatacgtttc ttttatttaa agaattatat gatatatcta
      961 atgaagaata taaaaaaaac ttggattact ttatcgaatt atttgaggta tctgactatt
     1021 taaatgttca agttaggacg ctttctttag gagaaagaat gaaaatggaa ctaatagtag
     1081 cccttctaca taatccaaaa atattatttt tggatgaacc aactataggc ttagatgcta
     1141 tagcccaaaa acaaattagg tatttcctaa aagaagtaaa tgaaacaaaa ggaactacta
     1201 ttatacttac ttctcattac atggaagata ttaagagtct ctgtagtaga agtattgttg
     1261 tagaaaatag tcgtaaaata tatgatggtt ctacagagaa attatttaat cattttcaaa
     1321 aatataaaaa aataacagtt agtttttcca atgaaactaa cattgtattt ccaaaagatt
     1381 gtaaagttgt tgaacaaaca aatcataaga catcagtaat tgtgccaaaa gaaagaggaa
     1441 atgaaacgat taaatggatt tttgataaat atgatgttaa agatgtaatg attgaagaag
     1501 aagatatagg tagtattgta gagagaatat ataagaaagg aagtgatgag tattatgagc
     1561 aactataatg aagcaagttc aaattattca atgttttata aatttaaaca gagtattaga
     1621 aggtattatg aagtagccaa aattaatttt aaagtgcaac ttatctggag atttgatgtg
     1681 gtggttaata tggtgtttac gattgtaaaa attttatttg cttatatttt atggaaagca
     1741 atttttggtg aaaataaaat agtctctggt tttacattta actctatgtt atcttactat
     1801 ataataagct catttttagc aggtattgat atgtcaaaga gtaccagtga agagatatgt
     1861 aatcgtgtaa aattgggcac cttttcaaag tacatgatta ttccaattaa tgttcaaaat
     1921 tattttattg ctaaaaattt tggaatgaca tgtttttatt ttgtatttaa tatgatttct
     1981 gcatttgtat ggatttttat atttaaaata gattttgcct ttacatcaaa tccagttcaa
     2041 atattaattt caattttgat gatttttcta gggtttattt ttatgatgaa actccacttc
     2101 tttattggga ttttgtcatt taaatttctt gagattagtt tttttagaat gataattgaa
     2161 aatattgttg aatttattac aggaacaatg ataccacttg tactcttacc agaaactata
     2221 gtaacagtta tgaaggtatt tccattttat tatacaacat atttgccatc tatgttacta
     2281 ataggtagaa acagcaatga ggcaatacaa ggattaatta ttctagcatt atgggtttta
     2341 tttttttctg tagtaaataa agttatgtat aaaagaatga gaacattatt tgatggggtg
     2401 ggtatatgat gaaaaaaaat ttgaaaataa tatttatgtt attaaaaatg aaattatcaa
     2461 agatgatggt atttagattt gatttttttg gagcattttt tgtagatagt tcattattta
     2521 tattacaact tctaatgttt aatagtatat attctcatgt agactcaata gggggatgga
     2581 aacaaggaga gatgttaata tttataggaa ctttctcatt aataaatgcg ataaatatga
     2641 cgattttctt ttttggtatt tatgatattc cacgaaaaat ccaggaagga gaattagact
     2701 actatattat aaaaccatta gatccactat ttaggttaac atttgaaaat attaatattg
     2761 gttcatcacc attaattcta gcaagtatac ttattgtttt atatggaata tctgagttaa
     2821 actcagagat aactttagct atgataataa tatatatttt catggtgtta ttgatgacat
     2881 tactttttta tgatgtatgt atcatactta gaacagtttc attttttgtt atatcatctt
     2941 ctgcgataat gagattagaa gataatttat tgccaatgaa tatgaaaatt cctggtgtaa
     3001 tatatgaagg tgtttttaaa gtattattct atttaatctt accatatgga attatgtcga
     3061 caattccgac tcaaattttg gctgggacaa taacaattaa tggattaata tactcaattt
     3121 taattgtatt tttgtttaca ttgtttatgt ggagattttg gaagttaggt atgagacatt
     3181 ataaaagtgc aagtagttaa taaattagta aaaatagaca atgtataact atatataaat
     3241 atgtttcata tgacactaaa ctatgtgaac aagtatttgc gctaaaggca ctgaaagcta
     3301 taaaattatt aaagtaagga tttaaaagtt tctttagtag aaatataatt gtgtaaaaaa
     3361 gcaagattaa ttaaaactat atccaaataa ttagatataa ttcttatgca gaaaggaggt
     3421 ctgtaaatgt ctaattgttt ggaatatatt ttaaaaataa ttaatgtaca gaaaaataaa
     3481 caaaatgagt gatgtatatt attcagaaaa tcatgaacaa gataatcttt atgagcaaga
     3541 taatttactg gcacaaagat ttaatttatt taccctattg aagtttgcat ttcctactat
     3601 ttttatgatg atttttatgg ggttgtatac aatcgtagat actatatttg tatcaagatt
     3661 tattaataca aatgctctat cagcaattaa tattgtatgt ccaattatta atcttatagt
     3721 tggaattgga actatgcttg ccactggagg aagtgctatt gtagcacgta agatgggaaa
     3781 tggtgatatt gagggagcaa ataaagcttt ttctttaatc gtattatcag gagttgtaat
     3841 tggtgtaata attactggta taggtattgt atttatggat tatatcatta tatggttagg
     3901 agcaagtgaa gttttaatta aatattgtcg ttcatatctt tttattatct tggtttttgc
     3961 ttctgcaaat atactacaag tattatttca atgtttattt gtaacagctg gaaagcctca
     4021 atttggatta tggctagtgt taggtgctgg gtgtgctaat ataattcttg actatgtgtt
     4081 cattgttctg ttacaaatgg gtattagtgg agcagcactt gcaactggag taggatacct
     4141 aattcctacc gtttcaggta tggttttctt ttcaaaaaac aaagggttac ttaagttttg
     4201 tatacccaga ctagataaaa gaatattatt tgaagcttgt tttaatggtt catctgaaat
     4261 ggtcagtcaa ctatcaacag cagttactac ttttttattt aatgctatga tgatgaaatt
     4321 aattggtgaa gatggggttg cagctattac aataattatt tattctcaat ttcttttaac
     4381 ttcgctttat ataggtttct caatgggtgt tgcaccaata ataagttata actatggaag
     4441 tgaaaataat acgcagttaa atcatgtttt taagatttgt atattattta tttcagtagc
     4501 atctttattg gtatttatct tttctatatc atgttcacca atcattgtag agattttttc
     4561 tcaaagagga agccgagttt atgaaattgc aaggtctgga ttttatatat tttcttttag
     4621 ttttattttt tgtgggtata atatttttac ttcgtctatg tttacagcat tatcaaatgg
     4681 aaaggtgtct gcaatattat cttttctacg tacatttgga tttataacta taggtttatt
     4741 gatattgcca aatatattaa atataaatgg gatatggtta gcagtaccaa gtgctgagat
     4801 tttaacttta tttttaacca ttttttgtat atgtaagtat aagaatagat ataaatatat
     4861 gtagatatta aataaatagt ttaggtagat attttatgtt ttaataagta attcaattag
     4921 tgggagatat atactattga aaattaaagt tataattgat ataaagaatt aataaaaata
     4981 acatataaaa gtttaaaagg gagttgaact taaagtttga ttagaaaaag tcaattttaa
     5041 gacaactcct tttattttac tattgtatta atctgtatct gtaagtgtat tagtctaata
     5101 ttataaactt tggatgaaat taattttcta taggttttaa tgtatcatac actttatttt
     5161 aaagaaactt atcttaattt tcatagtgat tgaatttgtc tattagagat gttactatta
     5221 gtaatgtaaa agcaagagta gtgaatataa aaaacaagat agtgatactg caagattaga
     5281 atataataaa tttttatgga gagatttcaa tatggataca tgttttaata aaaaaatatt
     5341 acttgttgat gatgaaaagg atatagtaga tttaattgaa gaagtactca taaatgatgg
     5401 ttttaaaaac attattaaag cttataatgg tctagatgct atttcgctgt gtaagatagc
     5461 atgtccagat gttgtcattc ttgatataat gttaccagat atagatggta tagaagtgtg
     5521 taaaaaaatt cgagagtttt catactgttc tattcttttt ttatcatcaa aaaatgatga
     5581 tattgacaaa atactaggtc ttagtagtgg gggagatgac tatataacaa agccattcag
     5641 tccaagagaa attgcttttc gagttaaagc gcaattacgt agacaacagt atcaaagcat
     5701 tgtaccatca gatagtgaag ttattaaaat aggagatatt actattgata tagaaggaaa
     5761 ccgtgtatat aaagatagaa atgagattga gttaactgga agggagtatc atcttttaag
     5821 ctacatggct caaaatgtaa ataaaattat aggaaaagaa agactctatg aacaagtatg
     5881 gggagtatat agtagtattt gtgacaatac aattatggtg catatacgac atataagaga
     5941 aaaaattgaa gataacccct caaatcctaa aatacttata acagttaaag gactaggata
     6001 taaacttgtg aatagaatcg attagaatag attttaatat taatgaagat atctcaagtt
     6061 gaatttttag ttaatgaggc attcttttat gggtgaaatc aaatgtattt tcgttatctc
     6121 aacaatgaag aagaggtcta tctctatttt gagatagcct cattaagttt atatcctggt
     6181 aatgtacttt atgttctaat aaaatttttt tagtttattt gcagtgaaac attaactaga
     6241 acattaaaat ttgcattgaa ataccaatta aataatattg tatattgtgt ataaacttat
     6301 actaattgtt tttctatatt aaatacttta tcaatccaac ctttataaaa tttttcttca
     6361 tgggaaacta ggattatact tcctctaaac tgtttcaaag cattttgtaa agcatccttt
     6421 gtctctgcat caaagtgatt ggttggttca tccagaataa gaaaattaca aggagataac
     6481 atcatacaac ataatttaac cttagattgt tcaccaccac ttaaagtaga tacactcctt
     6541 gaaacatgct cttccttcac accacatctg gctagatgac gtctaatctc ttttgtattt
     6601 aattttggat atttatctgc tactatttga agtggggttt tatcaggatt ttcccatttt
     6661 aaatcttgct cataataacc aattttaact tgttctgaaa actggaaatc tccagatata
     6721 cgaggaatat cttttactaa tgtctttaac aaggtagatt taccgattcc attaaaacca
     6781 gttataacta ttttttgacc tccatcaaca gaaaaattta attttggaag taaagaatag
     6841 tagtatccaa cttcaagatt tgttatagta agagcatttt gagccgatat ttcaatttct
     6901 gaaaattgaa tatttggttt tcctgtaaaa ctaggaggag ctattctttc tattttatcc
     6961 aattgttttt gtcttcctct tgcaattttt gaatttactc cagctttatt tttacgtata
     7021 aatgtttctt ctttttctat tttcttttgt tgtgcttgat accttctaat ataatcttct
     7081 cttaaatgtg atttttgttt aaggaactct gaatattttc catgatattt cttaatcatg
     7141 ccaaattcta tatcaagaat acctgtagaa attttttcta aaaagtcaaa atcgtgggaa
     7201 actacgataa atgccccatt aaatgtattt aaataatttg agagccattc aacatgttct
     7261 ttatctaaaa agtttgttgg ttcatctaat aatagaacat catggttaga aagtagtaat
     7321 tttgctaaaa ttacctttgc cctttgaccc ccactaagct ctccaacaac acgattcatt
     7381 cctattgaat ctaatccaag accatttgct attttattta tttcattatc tatagaatag
     7441 aaattacttg ctattagttg ttcctgataa tctgatgctt tgattagata ttgctcattt
     7501 tcagatatag cacttttttg atagagtaaa ttcatttcct tttctatttt ataaagttct
     7561 tcaaatgcag tgtgtaaata gagtgatatt gttgtatctc tattgatttc tgcatattgg
     7621 tctaaatggc ctatattgat attaggttgc cacttgattt ctcctgaatc tggaacaact
     7681 tctcccaata aaattttaat taatgtactt ttacctgtac cattttggcc aactacacct
     7741 atatgttcac ctttgaataa atcaaagtta gctttctcat acagtgattt atccataaaa
     7801 cattgtgata aattagttac ttttaataaa ctcataattc aaactccttt tcttttatta
     7861 aaaaatttat ggaaataaaa aaagcagagg aatggcattg taaaatgccg acctctgctt
     7921 ttggaaaaac aaaaggtcat agacaagcat tgttgtctat gaccagaaaa tctatattat
     7981 ctatcggcaa ataggtaatt ttaaataagc atagccaaaa aactatacta aacctatatt
     8041 tgccgatgaa gatagaaagc tttaagcttt cagattaacc tctacacaac gcttcatcgt
     8101 atacatttgt gtagagttaa gttttctaac aagtttaact aattctatat aaatcataat
     8161 tattcacatc ctaatcttag tattaattct agtttatcta tatttaaatt gtacttttgt
     8221 actttgtgga ttatcttatt tatttaaaat atgttttatt aattatagct taagttatgt
     8281 tatcataata cttagttata gtcaataaga aagttatatt tactattatt atattaattt
     8341 agtaaatcta tcattacaaa gttatttgaa gactcgctat caataaaatt ttatagtcta
     8401 taagaaaatt aagattatga gttttataat tttgatatag ggatactatt ttcataaata
     8461 ttacaacatg tagaagtata tgtctagtat tgttgaattt tcaatgatat attaaggttc
     8521 taatatatta tacaaaataa aaacattaat tcaacttaaa taattttaag tattatatgt
     8581 gttttgatta aagaaaacta aaattttaag gaaaaataat atatttgtat taaattttat
     8641 tgaaatgatt aaatttattt acttaatgta aattattagc taaaattgta ttaaataatt
     8701 aaggaggtga gtagctctgg atgaaataga tttaaaaata attaattctc taaaaaagaa
     8761 tagtagggct tcaacctctg aaataagcag acaaattaat ttatcagtac cagcagtctc
     8821 agagcgtata aggaaaattg agcagtcaaa tttaattcaa aagtacacta ttcaaattaa
     8881 tcgggaaaaa acaaaatata agcttttggc ttttatattt atcaatactg atttatctaa
     8941 aagtatgaaa gaatttaaga aaactatatt ggaatatgat actgtattag aatgtcatca
     9001 tatttcagga gagtatgatt acttaataaa agtattagca gaagatacaa gtagtcttga
     9061 agagtttata tcagtgactt tgaaggaaat tggggggatt caaaaagcta atacaaccat
     9121 tgttttatct acaattaaag aagaatttaa tacaataaaa gatatttttt aaataattta
     9181 ctagtaaagt gttaattata ttttaggagg aaaatagaat atgaaagttt tagttttttt
     9241 agcaaaaggt tttgaaacta tggaatttag tgtatttgta gatgttatgg gatgggctcg
     9301 taacgattat gggcatgata ttgatgtagt gacttgtgga tttaaaaaac aagttatgag
     9361 tacatttaat atacaagtat tagttgataa gacgatagaa gaagtatgtg ttgatgatta
     9421 tgatgctcta gctattcctg gaggatttga agaatttgga ttttatgatg aagcatatga
     9481 ttcatctttt ttaaatctaa taagggagtt taattcaaaa gaaaaaataa tcgcttctat
     9541 ttgtgtggca gcattgccag ttggaaaaag ttgtgtttta aaaaatcgta aagctactac
     9601 atatcatttg aaaaatggta aaagacaaag acagttaagt gagtttgatg taaatgtagt
     9661 taatgaacca attgttgtag ataaaaatat tataacctca tattgtccag aaacagctcc
     9721 acatgtagct tttaagcttt tagaaatgtt gacatctaaa gaacaaatgg atgaagttaa
     9781 attagcaatg ggatttaaac tgtaatggtt tatagatttt ctaagtaatc agaaccccag
     9841 tcacacatgg aatctaaaat tggaataata ctctttccaa aatcagatag cgagtattct
     9901 acttttggag gaacaacagg ataaaccact cttataatta aaccatctga ttctaattca
     9961 cgtaattgtt gagttaacat cttaggcgtt gctttaggaa ttaatttttt taattcattg
    10021 aacctcaata tagtgtcttt aagatgccaa agtataagtg tcttatattt accaccaatc
    10081 aatgcaagtg ttgcttctat tgggcaactg taattatcta aacatttttt catttttggg
    10141 ctcctttatt tattttaaag tatcttttta ggtactatat tacaaaaaag tgcattattg
    10201 taatatagta ccatagtgat aaaattatag cataggttaa ataaatcatc aacttagcct
    10261 aataaaacaa taggaggtaa ataaatgaat ttcgttgaac ttgcaaaaaa aagatattct
    10321 tgccgtaatt atcaagatag aaaggttgaa aaagaaaaac ttgagaaggt tttagatgtt
    10381 gcaagaatag ctccaactgg tggtaatcgt caacctcaaa gattgattgt tattcaggaa
    10441 aaagaaggta taaataaact ttctaaggct gccaatatat atgatgcgcc attagcaatt
    10501 cttgtatgtg gagataaaga caaagtttgg acaagacctt ttgatgggaa acaacttaca
    10561 gatattgaca caagtatagt tacagaccat atgatgttac aagcaactga acttggattg
    10621 gcaagtgttt gggtgtgcta ttttaatcca gatataatta gagaagagtt tagtttgcca
    10681 gataatctag aaccaataaa tatattattg atgggatatg aatctaagat accagagagc
    10741 ccagagagac atgaaaaaac tcgtgttcct ttaagtgaaa ttgtttctta tgaaactctt
    10801 taattataaa attaagttat tcattaggat aaatgtaata aagtgtttat tataaacaaa
    10861 attcaaaata aataatatct acatataatt aattttagtg aagtatatct agtttaacta
    10921 gaatgtatac ttcactaatt tatttattca aaatatttat gaggatttta atatattaac
    10981 taaaatatat caattgaatt aacttttaaa tctggaaaag cacttgaaat atagtgtttt
    11041 atgtcattta aatggtcttg aatttgcttt tctgatattt tatcaggtcc atatacaaaa
    11101 tacataatat ttttagtatc ttttgttata gctgttcgat tatcagggcc attaagaata
    11161 ctagataata taccaatatt atctgataaa aataaatcat cttttgttaa aatatgttct
    11221 ttaccaccta taccataaaa atgttcactt ccatttgcta attctatttt caaaggtaat
    11281 tccattttat caaggtcatg acctgctgtt agaagaagat ttttcaattc tgctaaaaac
    11341 attgactcaa ttgttacacc tacatctggg ataccttttg attttaatag aatagattct
    11401 aattgaagca atacagggta agttttcttg aattttttat aatactttat ataacttgaa
    11461 acaggctctg ttttaatttt ttctttgcga ttgtattctg ggtgttgttc tataatattt
    11521 ttaattgtag agtttttaag aactaaaaaa ttttctttat taggttttga atgtgtaata
    11581 tttataacca taactccaaa tttaatattg ggataaattt ctttaacttt atgtgatacc
    11641 tctaacataa tatagactcc tttttatttt atattttatg atataatctt agcaaattaa
    11701 aagtggattt tatagttcca tttttatagt aattttatag aaccactttt ggaggtatta
    11761 gaatgtggat tacgattaat agaaatagta atatatcatt atcaagacaa atttattgtg
    11821 agataaaaga tatgattctg gatggaagct taaagtttga gcaaaaactg ccatcatcta
    11881 ggactttatc aaaagaacta tctgtatctc gtaatacagt tttagatgca tataatcaat
    11941 taattgcaga aggatatttg gaaacaagga atggatatgg aacaattgta gctaaaggaa
    12001 tatcagggca taaagttgat tttaagttaa atgatacttt tagagatgaa aaagatttac
    12061 atactaataa acaaaaaaat tatatagatt ttcgttcagg agttccagaa ttaaaactat
    12121 ttccacgcaa agaactttca aagctatata aacaaatact gtatgattta tcagattcgg
    12181 atttcagata tggaagtaca gctggtgtat gggttttaag agtggaaata tctaagtatt
    12241 tatttcgtac tagaggaatt agatgtaatc ccaaaaacat tatgattgtt tctggttcaa
    12301 cacaaggttt atctttaatt tcacagcttt tatacaaaag taataaaaag gttgctgtag
    12361 aagaccctat tcataaaggc ttattaaagg ttatttcatc tattggatat tcagttgttg
    12421 gaattaaagt agatgataag ggaatgaata ccaacttaat aaaatcaata aaggggattt
    12481 cttttgttta tactacacct tctcatcaat acccattagg agggatttta cctattcaaa
    12541 gaagactaga gcttattaaa tgtgcaacag agaatgatta ctatataatt gaagatgatt
    12601 atgatagtga atttagatat gagggtcaac caattagttc tctttatgaa cttagcccaa
    12661 ataaagtaat ttacataggt tcttttagta aaatattatc tccagctatt cgtgttggat
    12721 ttatgttatt accagacaat attttagaaa gctataaaac cttaaaaatg tactctgatg
    12781 ttcatacaga aacaatattg caacatgtat tagctgaatt tattggaaat ggaggtttag
    12841 agaagtacat atttagaatg aagaagttgt acaatagaaa acgtaagtac ctcataaaag
    12901 ttttaaacac ggaatttaaa aatgaattta aaataaaagg tgaagcagca gggcttcata
    12961 tagttgctca tttctataat gttaaattta cggaaagttt aattcataaa atttttgaag
    13021 caggtataaa agtttatcct atacaacaat atgcaattga gaatatatta gaatatgacc
    13081 atgaaattat actaggttat gctcatctta gttttgatga aattgaaaaa ggtgtaaaaa
    13141 tactcagcaa aataatacat atgaatcaag gtacatagat aaaacatgta gcaatctaca
    13201 tataatagat gtaattaagt ttaaacttta aaagaaatat atattaatat ttttgtataa
    13261 aaatacctga tattttttta tcgatacttt attgctatac actaaattaa gatagcaatt
    13321 tatattaaga aaaaactttt tgcataggtt ataatactta aaaggagagg atagagttga
    13381 aaacaaatac agatgttatt atggagataa ttgattatat agaagaaaat cttacagaaa
    13441 atctaaattt agataaaata gcttatattg ctaattattc aaaataccat ttacacagaa
    13501 tgtttacaaa tgttgtagga tgtactatcc atcaatacat acagaggagg agacttactg
    13561 aagcagctag acaacttata tttacagaca aatatattat agacatagct attacagtag
    13621 gatattctac acatcaatct tttacactag catttagaaa tttatataaa gaatctccac
    13681 aagtatatag aaataaagat gaattttatc ctattcaaca gagatttgtt ttaaatctaa
    13741 atcatcaaat tcaaagtgaa aaatgtattt cttatgcaaa tatgaattat aaacaaagaa
    13801 gagatgaaat caattcttat cttaacttta atattaattg tgaggtgaaa gtagcatgag
    13861 tttagtgcca catgtaattg aacaaacagg tcaaggggaa aggtcttatg atatatattc
    13921 aaaattatta aaagatagaa ttatatttat aggagaagaa attaatgatg ctattgcaag
    13981 tttagtagta gctcaattac tgtttttaga atcagaagac ccagattcag atataattat
    14041 atatataaat agccctggtg gctctgctac atcaggattt gctatatatg atactatgaa
    14101 ttatgtcaga tgtgatgtat cgactatttg tattggtatg gctgcaagca tgagtgcttt
    14161 tttacttgca ggaggaacac atggaaaaag atgtgcttta cccaattcag agattatgat
    14221 tcatcagcca atgggaggag ctaaaggtca agcaacagat gtaaaaatag ctgtagataa
    14281 tatattgaaa ataaaagaac gattagataa aattctttct gaaaatacag gtaaatcaat
    14341 agaagatatt aggagagata cagatagaga taattttatg actgcactag aggctaaaga
    14401 gtacgggctg attgattata taatgaatag gaatgaataa agtatcaaag tatcatatta
    14461 aaatgtctgt tttacaaata tatatataat attaaaaaag agtgtctcaa aatgaacttt
    14521 ttagtttatg ggacactctt ttttgtatga aattaaatat ggttttatca tttcgacaat
    14581 tattaccaaa tttcaagttt aataaattct actataaaaa atacataaaa gataaggttc
    14641 tttagtaact ttctgtcgtc aaaagttttt ataaatttaa taacaaaaca agagaaaata
    14701 ccatatatta cttatgtctg caatatatag aagacaaagg agttgtacat tatgattaca
    14761 ataagtttgt gcatgattgt taagaatgag gaagaagtta taggcagatg cttagaatgt
    14821 gtaaaggata tagtagacga aattataatt gtagataccg gttctacaga ttccacaaaa
    14881 gaaatagtct caaaatatac agacatggtg tacgatttcg aatggataga tgatttctct
    14941 gctgcaagaa atttttcatt ttctaaagct tctaaagact atactatgtg gcttgatgca
    15001 gatgacataa ttttagaagc tgatagagaa aaattattaa agattaaaga aagcttggat
    15061 acttccattg atattgttat ggcaaaatac aatgtatcct ttgatgaaaa tggtaaccca
    15121 actctctcat attatagaga acgattgttt aaaagaagtt ctaatcataa atgggttgac
    15181 cctattcatg aagtagtacc tctttttgga gaggtatttt attctgacat tgcaatatct
    15241 cataaaaagc ttcatagaca ggaccctcta agaaatctta gaatttttga aaaaatgatt
    15301 tcagaaggaa aaacattgga gccaagacac caattctatt attctagaga gttgtactat
    15361 aatgctaggt ataaagaagc aatagaaggt tttactaagt ttttagactc atcaagagga
    15421 tggattgagg attgtatcag tgcttgcaga gatttagcca catgttatta cttaattaat
    15481 gatgaaaaaa gtgccttata ttcattgttt agaagtttcg aatttgatga gccaagagca
    15541 gaaatttgtt gtgacatagg aaagcatatg tttgatagac aaaaatataa agaagccatt
    15601 ttttggtata aggtagcatt aacaagagat aagaatgata ctaatggagg ctttaagtca
    15661 aatgattgtt atggatatat accatctata caattaagtg tctgttatga caggttagga
    15721 gaatcagata aagctattta ttatcatgaa aaaacaaaag aaatcaaacc taacgatagt
    15781 gctgttttgc acaatgaaaa ttattttagt aaatttaaaa atagttgata ggtcacaatc
    15841 atactaaatt tgacatatta tatatagagc ttaaaagctc aaatatatca ggaggtgctt
    15901 ttaataatga gtagaaataa atattttgga ccatttgatg ataatgatta caacaatggc
    15961 tatgataaat atgatgattg taacaatggt cgtgatgatt ataatagctg tgattgccat
    16021 cattgctgtc caccatcatg tgtaggtcca acaggcccaa tgggtccaag aggtagaacc
    16081 ggcccaacag gtccaacggg tccaacaggt ccaggagtag gggcaacagg cccaacagga
    16141 ccaaccggtc cgactggtcc aacaggaaat acagggaata caggagcaac aggattaaga
    16201 ggtccaacag gagcaacagg ggcaacaggc ccaacaggag cgacaggagc tatagggttt
    16261 ggagtaacag gtccaacagg cccaacagga gcgacaggag caacaggagc agatggagta
    16321 acaggtccaa caggtccaac aggagcaaca ggagcagatg gaataacagg tccaacagga
    16381 gcaacagggg caacaggatt tggagtaaca ggtccaacag gcccaacagg agcaacagga
    16441 gtaggagtaa caggagcaac aggattaata ggtccaacag gagcgacagg aacacctgga
    16501 gcaacaggtc caacaggggc aataggagca acaggaatag gaataacagg tccaacagga
    16561 gcaacaggag caacaggggc agatggagca acaggagtaa caggcccaac aggcccaaca
    16621 ggggcaacag gagcagatgg agtaacaggc ccaacaggag caacaggagc aacaggaata
    16681 ggaataacag gcccaacagg tccaacagga gcaacaggaa tagggataac aggggcaaca
    16741 ggattaatag gtccaacagg ggcaacagga acacctggag caacaggtcc aacaggagca
    16801 acaggcccaa caggagtagg agtaacagga gcaacaggag caacaggggc aacaggagca
    16861 gacggagcaa caggagtaac aggtccaaca ggagcaacag gggcaacagg agcaaatgga
    16921 ttagtaggcc caacaggagc aacaggagca gcaggaacac ctggagcaac aggtccaaca
    16981 ggagcaacag gcccaacagg agtaggaata acaggagcaa caggggcaac aggagcgaca
    17041 ggtccaacag gagcagatgg agcaacaggt ccaacaggag caacaggaaa tacaggagca
    17101 gatggagtag caggtccaac aggagcaaca ggagcaacag gaaatacagg agcaga
//
LOCUS       DAGSWI010000087        17020 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.087, whole genome shotgun sequence.
ACCESSION   DAGSWI010000087 DAGSWI010000000
VERSION     DAGSWI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.087"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..490)
                     /locus_tag="MJF80_002578"
     CDS             complement(<1..490)
                     /locus_tag="MJF80_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBZ0281232.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VET"
     gene            complement(555..1307)
                     /locus_tag="MJF80_002579"
     CDS             complement(555..1307)
                     /locus_tag="MJF80_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBZ0281233.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1338..2636)
                     /locus_tag="MJF80_002580"
     CDS             complement(1338..2636)
                     /locus_tag="MJF80_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBZ0281234.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYITKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2653..4917)
                     /locus_tag="MJF80_002581"
     CDS             complement(2653..4917)
                     /locus_tag="MJF80_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBZ0281235.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5172..5990)
                     /locus_tag="MJF80_002582"
     CDS             complement(5172..5990)
                     /locus_tag="MJF80_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBZ0281236.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(5983..6681)
                     /gene="cbiQ"
                     /locus_tag="MJF80_002583"
     CDS             complement(5983..6681)
                     /gene="cbiQ"
                     /locus_tag="MJF80_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBZ0281237.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6684..6962)
                     /locus_tag="MJF80_002584"
     CDS             complement(6684..6962)
                     /locus_tag="MJF80_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBZ0281238.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(6952..7704)
                     /locus_tag="MJF80_002585"
     CDS             complement(6952..7704)
                     /locus_tag="MJF80_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBZ0281239.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(7886..8078)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8210..9982)
                     /locus_tag="MJF80_002586"
     CDS             complement(8210..9982)
                     /locus_tag="MJF80_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBZ0281240.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(9996..10925)
                     /locus_tag="MJF80_002587"
     CDS             complement(9996..10925)
                     /locus_tag="MJF80_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBZ0281241.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11154..11342
                     /locus_tag="MJF80_002588"
     CDS             11154..11342
                     /locus_tag="MJF80_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0281242.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11335..12036
                     /locus_tag="MJF80_002589"
     CDS             11335..12036
                     /locus_tag="MJF80_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281243.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12038..12961
                     /locus_tag="MJF80_002590"
     CDS             12038..12961
                     /locus_tag="MJF80_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0281244.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13118..14041
                     /locus_tag="MJF80_002591"
     CDS             13118..14041
                     /locus_tag="MJF80_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281245.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14034..14771
                     /locus_tag="MJF80_002592"
     CDS             14034..14771
                     /locus_tag="MJF80_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281246.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            14782..15555
                     /locus_tag="MJF80_002593"
     CDS             14782..15555
                     /locus_tag="MJF80_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281247.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16096..16704)
                     /locus_tag="MJF80_002594"
     CDS             complement(16096..16704)
                     /locus_tag="MJF80_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBZ0281248.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(16882..16998)
                     /gene="rrf"
                     /locus_tag="MJF80_002595"
     rRNA            complement(16882..16998)
                     /gene="rrf"
                     /locus_tag="MJF80_002595"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5465 a         2971 c         2165 g         6419 t
ORIGIN      
        1 aagtttctac agtaacagat ttcataattt gtggagtttg tggcttgtca gctgaatctg
       61 ttctaactga tgctatttca tcaactgtat ccataccttc tatcactcta ccaaaacctg
      121 catactgacc atctaagtgt ggtgagtttt tatgcattat gaaaaattga gaaccagctg
      181 aattaggtgc catagttcta gccattgata tgactcctct ttcatgtttt aaattgttag
      241 taaatccatt accactaaat tcacctttta ttgagtgacc tggaccaccc attcctgtac
      301 catttgggca tcctccttgt atcataaatc ctggtattac tctatgaaat attattccat
      361 tgtaatatcc ttcatttact aatgatacaa agtttttaac tgtatttgga gctatatttg
      421 gatataactc aattttgatt tcttttccat tttccatttc tatagttact ataggatttt
      481 tattttccat aatttattac cttccttttc attagttaaa ttacataatt acatttatta
      541 tattatactc taaattaaag tctatcaagt atattttttc ctatttcata tccttcttga
      601 taaatagttt tacaaaactc atcatcaaag ttaagcgtgt ctttaggagc aaattgagta
      661 ttaggtctaa ctactattat tttatcttca tcatatactt ttttaatatc ttctggcaac
      721 tcataattat attttgtgga tattaaaata attttatcca cattatcccc aattaatggt
      781 tcaattaatg cacttctaat taaacctcca tctaatttaa aaccatcata aattccttct
      841 tgcacatctt tcacaaattg ttctttaaat ccaagagttg cgtttggatt tgatggtaac
      901 aacccactgt atgaaatgct gataatggct tcattacgag gcatttttga tacgtcaaca
      961 atcttttctg atactacttt attatcaact tcaacataat taacataaaa gttcatttct
     1021 tctatattac tatcatctag ttcccttagg ttgtctatag caggagaatt atccacagta
     1081 ttatttacta tttttacatt tccattctgg atatctttta tatttgtcca cattttctcc
     1141 aagttatcaa cattccctgt gtacaaataa tatccattta ttgctcctat tgatgtccct
     1201 gaaatcgaat aaaatttatt tattcctcca tcatacaagg ctttaactac tcctgcttga
     1261 taagcccctt ttgctcctcc accttctaaa cataatccta atttcatagt ctcatctcca
     1321 aattgtttat cttatattta tatttcaaaa aacttactaa atgttttata tatactatag
     1381 tggtcctcaa catttcctaa ttctcttata gaatgcattg ctaaaattgg acttcccaca
     1441 tccacagatg gtatatctat atgagtagaa gatattggac ctatagtact tcctcctctt
     1501 tcatctgacc tattaacaaa ttcttgatac tcaacattag cttctctaca tatattttta
     1561 taaatagcta tagaatatcc atcactagta tatgcctgac tagcatttat ctttataacc
     1621 ggaccttttc ccattattgg tttattagtt gggtcatgct tttctggaat gtttggatga
     1681 atagcatgag ctaaatctga tgatatcata aaagatgaat aaatagactc aaagaatccc
     1741 tctctatcct tacccattga tatgcaaatt ctctccaata tatttaacaa catgtttgaa
     1801 tctgcacctt gtttagtaga gctaccaact tcttcattgt caaatacagc cattacattt
     1861 acaccatgtt gacctttagt attaacaaga gcattcaaac tagcatgtac cattgcaaga
     1921 ttatcttgtc tcccaataga tataaattca tcattagccc ctattaaact tcctttttca
     1981 aattcatata aaaataaatc aaaatctata atatcttcca tatttacatc taagttagat
     2041 gctatttgct ttataagaaa attatctttt tctaatgtat catttaacat cccaactaaa
     2101 ggtaacatat ctttttgctt attatatgag tgaccatcat ttatacttct atttaaatgt
     2161 attgctagat ttggtattat acaaattggc tttttaaagt ctataagcct ttcatttggt
     2221 tttaatatac tatcaccttt taaaactact ctaccagcta tcccaagagg tctatctaac
     2281 catgtactta atattgctcc tccatagcat tctgtattta acttcaaata agatttttca
     2341 gcttctattt ctgcattcgg tttaatacta aatgtaggag aatctgaatg agagcctatt
     2401 attctaaatc catctctttc tataccttct gaatttacta caaaagctac tatagctgat
     2461 aaattcttag taatataata ctttccacca actcttagat tccatctttg attcaaaact
     2521 aattcttcaa atccattttt atctaagatt tccttagtac tctcgactgc atgaaaagac
     2581 gacggactat catatatgaa gtctattaag tttttagcaa agtttatact atccattcct
     2641 catttacctc cattataaaa tatttagtgt tgtatattct ttatttattt ttttaattcc
     2701 ctgttggtca aaagctatag taacatctgt accaattata gaaaccactg tcccaacccc
     2761 aaatttagga tgatgtacct tagaaccaag ttttatatca tctggattag tttctttatt
     2821 agaatctttt attgtagcat taactttatc tgcaacttta gatttgctta tagtactcat
     2881 atacttttta gtatatttat ctaacatatt ataacttgct ttagaatagc ttagttcttt
     2941 ttttactttg tacaatcttt ctatacattc ttctggcagt tcttccataa atcttgaagc
     3001 tattgcaaca tttgttttac catataatgt tctcttctct gtcaatgaca tatatagctc
     3061 ttctttagct ctagttatac caacataaca aagtcttctc tcttcttcta tttgtgaatc
     3121 actcatagat tttatagctc ttgatatagg aaataaccct tcttccatac ctgttaaaaa
     3181 cacaacaggg aactctaaac ctttagatgt atgaattgtc ataagcgaaa ccctatcatt
     3241 gtcttcttca tcatttgatt cagatgtaag tgctatactt gttaggaatg tctctaagtc
     3301 tttattttca taagtatcat cattactact ttcaaactcc aaagctatag aaataaattc
     3361 tctcaaatta tcaattctgt cttgagcttc ttcacctttg cctgttaaat ctttttcatt
     3421 tttatttctt atctctacaa gctcgtccat atatcctgta gtatccaaga ctttttctat
     3481 aagtttgcta acaggataaa cttcttttat agttctaaga gtccctataa tatcaacaaa
     3541 tccacttata cttgcccttg ccttagtaga tatatctgaa tttgtttcta tatcaattaa
     3601 tactgagtat atactttcct gttttaagtt agctctatct tcaatttttt ctatagttct
     3661 aagccctata cctcttctag gaacatttat aatccttttt atagatatat catcttgtgg
     3721 attttgaata actcttaaat acgccaacaa atcctttatt tcttttctct catagaattt
     3781 tgtaccacca tatatattgt atggaatttg acttctattt aaagcgtcct ctacaggtct
     3841 tgcctgtgca tttgctctat aaagtactgc aaaatcctta taaggtctat tttcttttct
     3901 agctatttgt gcaattgaat cagcaatgaa atcagcctct tctatttcgc taccagttaa
     3961 ttgtatcttt ataagttctc cttctttttt ttcactccaa agcttttttc tttttctttc
     4021 tatattatta gatatgactt tattagctgc atctaatatc acttgtgtag acctataatt
     4081 ttgttctaac tttacaacat gtacattgtc ataatctttc tcaaattcaa gtatgtttct
     4141 tatatctgca cctctccatc cataaatact ttggtcatca tctccaacaa cacaaatatt
     4201 ttgatgttct cttgctaata attttatcaa ttcatattga gctttacttg tatcttggta
     4261 ctcatctacc attatgtatc taaatctact tctgtaatat gctaacactt catcattagc
     4321 cttgaaaagt tctacagttt taagtattaa atcatcaaag tcaagtgcac tatttctttt
     4381 taaacggtct tgatatagcg cataaatatc tgcaatctta accattctat tatctgccat
     4441 attgatatct ttaaactgct ttggagtata aagtttgtct tttgctcctg aaattgcact
     4501 tataactgct tttggttcaa acactttatc acttaaattt aattctttta gacagtcttt
     4561 aaccaatgta acttggtctg ctgaatcata aattacaaaa cttctattgt aaccaatctt
     4621 atttatatct tttcttagta tacgaacaca gcaagaatgg aatgtactta tccacatatc
     4681 cttagtttca ggacctatat tttgttcaac tctctctctc atttcatttg cagctttgtt
     4741 tgtaaatgtt atagctaata tatttggtgc ttgtacacct ttatcttcta taagatatgc
     4801 tattcttgtt gttaatacct tagttttacc tgaaccagca ccagccagta taagtactgg
     4861 accttcagtc ttttctactg cttctctttg agcaggattt aatgtatcta aattcatatt
     4921 tctcctcctg ttaacagcca taaatatatt ttaatctaaa atatgaatta cttcaaatta
     4981 taattatgta ttatattaat agattatttc caatatatta caattaaata acatttttcc
     5041 atataattta tttcattgtt taataaaaaa aataacctta tgatttgggg aatcataagg
     5101 cacctttaag gggtctaggt taagggttat tatatataaa tctatttttt taaaataggt
     5161 ttagggctta attatttatt tgtattaagt tctctttcct tccaatactt atacaaatct
     5221 tcttctttct taaataaggg taaattcata tttctatgta cttttactaa ccatggggat
     5281 tctaggccag cttgtataat attattttca tttataaaca cattttctgc ttttccttca
     5341 tcaatcaaaa tccccttatc caatacatat atgtaatcac atatttcata cattaaattc
     5401 atatcatgac tggatattac tatttttata ttatttttat ttaatccctt tattatatct
     5461 acaatagacc tagtacttac tgggtctaaa ccagctgtag gttcatcaag taatacaatt
     5521 tcattttcca ttgctataac tgatgctatt gccactcttt ttttctgtcc atagctaaga
     5581 aaatgtactg gcctgtctat aaaatctatt ccattaacag ctactagagc tttgtttatt
     5641 ctctctttaa ttgttttttc atccatacca atgtttctca ttgcaaaggc tatatcatca
     5701 tatacttttg aataaaaaat ttgtttttct gggtcttgga agacaatacc tacatttttt
     5761 ctaaggttgt ataaacttct cttatcatat tttaactttt cttctttaaa aagaatttct
     5821 ccagatgtag gctttaatat acccattaaa ttcataaata atgttgattt tcctgaacca
     5881 tttgaaccta ttatacctat aacatttccc tttgaaaaat ccatattaat gtttaatagt
     5941 gcattggtat ttttttcata ttgatacgtt aaattattta ttttaaacat cattatcccc
     6001 tactatgtga aatttaccat catacaattt tatgtctaat gatatacaca tatcatcata
     6061 ccttgtcata agtcttttgt acagcatact tccaagaata cctaaggatt tatatgaagt
     6121 ttttaaattt atatatccaa atcttagctc ttgtgacttg tatatttctg aaaattcttc
     6181 taaaaatatg aatataaacc tatatattaa cattgataac tcgacaaaag tgtcaggtaa
     6241 atgaagtttt ttaaaaaaga atattaattg attaaaaggt gttgtaagta tacaaaaata
     6301 cacacaagtc aaacaagaca tcgctctaaa taacacatga atagacatgt caatagacgc
     6361 ctttgatatt cctacattaa aactaaaaaa ttcaaaacta tataataaat ctgctttatt
     6421 aaaagatata tttacaagag ttattcctat actaagaaat aaaaagtaca ttggtattct
     6481 aagtaatttt aaataatttt ttaaatctat tccagccaca caaacaacca aaatactcat
     6541 taaaatcata attccactta aaataaatat attttgtatt accatagaag caatcagaaa
     6601 aattactcct atggctactt ttttgtttgg atttacttta ctcaatctat ttgtatatgc
     6661 atatttatca attataagca tgattaactt ctctttcctt tttgaagacc taatatgtat
     6721 cctattacac cagcccctaa agctgcttgt gcagagaata ataaactttc tatttctcca
     6781 cttggtggct catatggact gctaaaccat ggtttgtaat ttggatttat cttagtaatt
     6841 tcactttcag cttgaccatc tgctcctcca tattcagcac ctgagttgac aagtaatggg
     6901 aatataataa gagctactac taataaaagt aataatatgt tagtttttgt tttagcactc
     6961 attagcttta actcctttat cattattttc tagtatgctt ttttctccgt tttctgtaat
     7021 taaattatat acaattacag taagtatacc ttctgctatt gctattggta tttgagtcat
     7081 aaagaaaatt cctaaaaatt taattgctga agccattacc ccacctgatg ggtctgggaa
     7141 agccagtgct aattgtattg aagttattgt atatgtggct aagtctccta ttgctgctgc
     7201 aagaaatact ggaatagcat tatttccatc ttttttcttt aaagccttaa atattaggaa
     7261 tgatacgatt ggccctatta tcgccattga aaaagcatta gctcctaaag tagttattcc
     7321 tccatgtgct aataacaatg cttggaatat taaaactata gttccaagaa caaacattac
     7381 gcttggtcca aacattattg ctccaagacc tacaccagta ggatgtgaac aacttcctgt
     7441 tactgaaggt atttttagtg ctgataatac aaacacaaat gctccacata aagctaataa
     7501 tactttcttt ttagggtctt gctttactat tttacctatt gattttaatc cgaataagaa
     7561 aaatggtata aatataactc cccatgctat agaccatttt actggtaagt aaccttccat
     7621 tatatgcatt gcatttgatg taactggagt taaaatgatt aacataagtg cagctataac
     7681 ccctaattta atattctgtt tcatcttagt atccctctct ccctttctaa taattaaaac
     7741 ataataatac atgtctgatt tatttaataa aataaaaaag cctaactcga tggttaggct
     7801 tctaattttc cacaatctaa atacaaacac ataaaacaaa catatctgta taattattat
     7861 aagaaacttt ctccatcccc gaagaaatta ctttaatatt ttaggcaggt ctcctgactt
     7921 agcttcatcc tactcctctt ccttcccatg agatataaaa aatatcatca tagtggctaa
     7981 caaaagtttc gtctgcatca cagtagcggg ggctgtaaag gaatttaacc tttttcccta
     8041 ttaatcttgc ttaaagaacc tataatatta tatatttaat ttttaaattc ttcttaaatt
     8101 attttctata taaatcattg tatacctttt ctgtgaaaat ttctacattt tttataaatt
     8161 ttattcattt ttatattttt atgtatataa attctttttt gtaagtttat taatttctaa
     8221 ttatcttctt taaatacatt gaaactacat cagaaaatct agttatatct cttgaatata
     8281 taaaatcatt tccatatatt aatctttcag cttcaagttc tgattctttt cctgtaaata
     8341 ctcctaatac taatatattt tgctttctag cttttctcac ttcattggct gtatctctca
     8401 aaccaactat acccctataa gccttttctc ctcttaatgt tctctcacta ctttttccta
     8461 tctttacatc atttggtctt ccatcgctga gaactatcag gattttattt tcttcatctc
     8521 tatttattag accactacat atagatttta ttgccaaacc atctctatta ttaccagcac
     8581 aaaaatattc aaatatattt tcactatttt ttatactatc atcataatct ttaaatcttt
     8641 ttaatattgt atagtctaaa aaactagaaa atcccatcac tctatttggt atgccaactt
     8701 ctgttagagc atttgcaatt atgtaagctt gaattgccac atttccctga tttctacttt
     8761 gagagccact tgcatccaat aatatatcaa taacgtattt ccctttttca ttttctatat
     8821 ctttataaaa tattttatta ttattacttc taccaactct ccaagctcta tttgctaata
     8881 tagtaccaca atcagaatat actctacttt tagcattttc ttctattaaa attcttgata
     8941 gactttcttt taatctcttt atttgctgcc tatacattct agcatgttct ctaaattttc
     9001 ctaaattatt ttctttttgt cttgttacat attttagttg aaatacatta ttacactcac
     9061 ttctaagaac tccatctgta aagtgtattc tacaaccttc atgaacattt ctacaatttt
     9121 gtgtctcaat ttttttaatt tcacctttac tcaaataagt ttttccataa taatgttcta
     9181 tttttgaata tatcttattt gcagtgtctt catcaacata tataactcgg tctgctgaat
     9241 tgctacattt agaatcatca aaatccccta caccttctac aagcatggat gaagatattg
     9301 aatctatatc actttcaata atattttctt cttcatcact ataaagttct tcatacatga
     9361 aatcagcaaa tgtatcaaaa tttacttcta cactttctac attattttca tcaataagac
     9421 tattttcttc tccatttaat atgtattcaa aataagttaa atatataaca tctaatgctt
     9481 ttaaaatctc cataatatct tgattggtat ctatatttct tatatcatta actattcttc
     9541 tagtcatact atctaatgct gggtgcttgt ccatactata aagtacaaat gtgtatttaa
     9601 ttttatccat aatattatca ttgtgtattt tggtaaaacg tgaaacaata tctttataag
     9661 catttcttct tatgtcttct atccctggcc tatctttaat gaccttttct tcaactattg
     9721 tatttaatat aacttgaatt agacccagta taatatcttt gttgtatcct tttcttattc
     9781 tatttgtaag atatttttta actatatccc aatctacata ctttcttcta gcacctgcaa
     9841 ttattgcaaa atatagacct atttcttttg aagagtaatc tttttctgat atatctatac
     9901 tttcattgta atcaccagct attgtccatg ataagttatt agctctattt tcaaattcat
     9961 aatcatcata tatccatttc ataatctcac ctatattaaa tatgacttat tggaaacaca
    10021 tctatactct cccatttatt aggtattcta gttttaatta catctccaac catttctttc
    10081 tcatatacat caaaagtctt tcctgtaagt cccatattaa ttgcaagagt tggttttaat
    10141 cctcttctta tagtcttaag agatgccata agaccacgta aatcaatagc tttactagat
    10201 atctctccat tttgagactt caattgtaaa tctaaaaata ttcctgcaaa gtgtattagt
    10261 ttttcttcat cagcatctga aaattcattt tttaaaatca tcattaactt atcttcttct
    10321 ataggaggaa tatctattac cataaatcta gataccagtg cttcattaag ttcttttgtt
    10381 cctgcatatt cataattcat agtcccaata aatcttgttg ctggatgtag attgactctc
    10441 tcatacccag gtacatcaat tattcttcta tagtctaaag ctgaatgtac cacaactata
    10501 gcatcatttt ttgccatatt aatttcatca aacaccccaa aacctccatt aatagcacac
    10561 tcataaactg agcctcttct aagtttaact tcattgtcta taaatgtatc tgtacctata
    10621 agtgttgaac tgtctgtatt tatatgaaat gatgtattcc actgaggtct attaaatatt
    10681 tcccctagat tatcagcaag taagtttttt cccgttgcct ttggtcccga taataatata
    10741 ttttcttctt ctaaaatagc tgctatacac atagacaaga tatctttacc ataaaaataa
    10801 gttttagact ttgttactct gtattctaaa tctttttcta atccataata atttctaaaa
    10861 tacagtacat cttcaataag tttttcatct accccttgca cccttagatt atctattata
    10921 tccatatttt tacccctttt atataaatag tatttgtttc atttcttatc accattttag
    10981 cataaaagga tataagctaa aattgatttt ttaatctccc ccattttaag taaaaccatg
    11041 caccatttta tcactacaaa attaagagta ctacttactg atatttcttg attttattcg
    11101 tctatacgta tataatatat actgataaat tatgaggaaa ggatgattga aatatggaat
    11161 atatgtcttg cccagaagta gcaaaaaaat ggggtatttc tgaaagaaga gtacaaaaac
    11221 tatgtgaaga aagtcgcata ccaggagttt caaaactcgg gtatatgtgg ctgataccaa
    11281 agaacgcaga aaagcctgtt gatggtagaa caaaaagaag aaaggaagta caatatgaat
    11341 agcgttttga ttatagatga tgacaaagaa ctttgtgctc ttatgaaaaa atgtgttgag
    11401 caagaaaatt tatctgctat tactgcacat ggtggtgcag aaggtctaca tttagtcgat
    11461 aaaaatagac acacactctc tcttgtaatt ttggacataa tgatgccaga tatagacggt
    11521 tttcaagttc tgaaaaaaat tagagaaaca agtaatattc cagtaattat gcttactgct
    11581 aaaaatgacg aggatgataa agtttccgga ctacgtttag gtgctgacga ttacctaaca
    11641 aaaccgttca gcataaacga acttatggct cgtgtcaatt ctcttattcg tcggtatact
    11701 atgctaaacc ctgcttttat aacagacaca gatttcataa taataaatgg catgattatt
    11761 gacaagacaa accgaattgt ttctgtcaat aatattcctg ttcaacttac aagcaaagaa
    11821 tttgatttac ttgcttttct ggcttctaat aagggacggg tgtttaccaa aaaacagatt
    11881 tatacacaag tatgggaaga agaatatgct tttgacgata gtaacatcat gtctttcatc
    11941 agtaaattga gaaaaaagat tgaaccagac cccaaccatc cattttatat tttgacagtt
    12001 cgtggtgtcg gttatcgttt taataaggag gcttaacatg aatacaagtg tctatctgct
    12061 tttgatattg gctcttagcc tgattattat agcgtacctt gtttctgtcc tgctacgtgt
    12121 ccgaatacaa cttacactta taaaagatgc tttagaggat ataaaaaatg gaaaccttaa
    12181 tcgacgtatt ttaacagatg aaaacgacat tacaaaacaa atttgctatg atattaacga
    12241 aatagcaatc aatagccaat ttcaacttat tcagcaaaaa caatcagaac aagcctacaa
    12301 acagctaatg acaagtctct cccatgatgt aaaaactcca cttgcttctt tggtcggtta
    12361 tttggaagca gtagaaagta aaattgttgt aggagaggaa aaagacgagt atatccatgt
    12421 tgcgtctaat aaggctcatt acttaaagaa ttttgtagaa aatctatttg aatgggtaaa
    12481 attggattct aaggaacaag tttttcattt tgatattttt gacttgaatg aattatcccg
    12541 taatattata tctgattgga tacctgtttt agaaagcagt cattttgaat atgaatttga
    12601 tataccagaa atagagtact tcttacgtat agacgcaaat gcatataccc gtatcattaa
    12661 taacctatta caaaatatta ttacccatag tagtggcaac aaaatgacat tgagaatttt
    12721 tgaaaacaaa gagcaagctc aaattataat aacagataat gggaaaggta tatcttcaga
    12781 caatctccca catatttttg aaaggttgta ccaatgcgac cattcacgag cttcaaaggg
    12841 aaatggttta ggcttggcga ttgcaaagga acttattaac gctcataagg gaaatattac
    12901 cgccaacagc actcccggta tgggaacaga atttacaata ttgcttccta aagccctgta
    12961 aaagtacgag gctttcttac tctaaaacaa gataaaagca agattatggc aaggttttgg
    13021 caaggttcat gttttataat tctaattgta gttgattttg acgttgtatc aactatcttt
    13081 tgtagaaaca aatttattaa aagtgaggaa attatatatg aatgattttg tgattgaaac
    13141 aaagcaactt acaaaaattt atgggaatca aacagtagtt gacaaagtca atctccatgt
    13201 aaaaaaaggc cgaatatatg gtctgttagg acgcaacgga gctgggaaaa ccacaattat
    13261 gaaaatgatt ttaggattga cttcaatcac gtatggcgag gtagatgtat ttggagagaa
    13321 tattaaggga cgtgaaaaac aagtatatcc ccgtatcggt gcaattatcg aaacgccagg
    13381 attttatcct aatctgacag gaacggagaa cttggagatt tttgcaaaac tgcgtggtac
    13441 agccgcccct aattctgtaa aaagagcatt ggaaattgta ggcttacctt ataaaaataa
    13501 aaagctgttt agtaaatatt cacttggcat gaaacagcga cttggtattg ccaacgctat
    13561 cctgcatgac ccagaactgt tagttttaga tgagccaacc aatggtcttg accctattgg
    13621 tattgcggag atgagaaagt ttattaggga cttgagcgtt gagcatggaa agacaattct
    13681 tatttccagt catattcttt cagaaatctc attgttggct gatgatattg gaattatcga
    13741 ccacggtgtt cttttggaag aaagtagtat gagtgaacta gaaaagaaaa acagtaaata
    13801 tatcctgcta caagtttcag atattccgaa gacatcactt attttagagc gtcaatttaa
    13861 cacgaaggat tattccgttc aagatgacta cacgttacac ctttataaca ctaatctgga
    13921 tatgggtgaa atcaataaag cacttgtaat gcagaatgta actgttatta gttctcaact
    13981 ttgtaatgat actttagagg actactttaa aaaaatcaca ggaggagagg gcattgctta
    14041 aacttattca aacagaattt cttaaactgc gtcgtaggaa acttatttgg ctgatgttgt
    14101 tgtctgctct cattatgccg tttttcgcac tactatactt caattatttt ggtaaaacag
    14161 gtgttgagcc aataaagttt tataaatggt ctgcatttgg ttatacactt tggattattt
    14221 tgcctgttgt tttgggtata ttttgcacca tgcttataca cgaagaaaac cagtatgata
    14281 tgctcaaaca gctttggatt gtacctatta gtaaaatggg atactttttt agtaaatttt
    14341 tcgttgtttt gatttactct atttgtttta tgctgattac agccgttgct tctgtgttat
    14401 ttagtgtact ttctggatat gtcgtattta gctgggcaag cattttatat ttgttgaaaa
    14461 aatgtttaga aattggtgta atcactgctt ttgtaatgtt gccgattttg gcaatcgcta
    14521 catctcaaaa aggatacatt ctcccaattt gtatcacatt ggtatatgcc ttttccggtt
    14581 ttgttataat gacaataaat atgtatttac acccattatc cagtatggga gttattatca
    14641 tgagaaataa agacattcca ggtcttatat tttcacagga aattaatgtc ccattagcat
    14701 ttctttgtat ttgtatttgg agtattgtat cagtattgtt tgcaaatatt gcactaggaa
    14761 gaagaaagtg aggtgtagat aatgtggaaa ttactttggg ctgaatgtca aaaaattcgt
    14821 cgttcaaaaa ttgtttggat tacagttttt gcatccatta tgatagcagt catagttttt
    14881 gcaggggggc aggatgtata taatgaccca gatttacatt atggcttaaa gagcgtttat
    14941 gacggttcac ggtatataga caatgcaggt tggtatatgg acgcagtaca accttggtca
    15001 actttttttg ttcttcctgc tgtcattgcc ctattaggaa gctatattat ttgtcgtgaa
    15061 gaagaggagg acactattaa atccctgcgt cttattccca taaatgaaac aaatttaacc
    15121 atagcaaaaa tgattattac ttttatattc agtatacttc tttatttgtt actctttact
    15181 ataacatttt tgactgaatc cgttttacat ttttccgatt tatcgacaaa attggtttta
    15241 agttgcatga aagagtattt tcttgatggc ataggtgtat tctttgcaat ttctcccatt
    15301 attgccttgg tatcgcgcat gaaaaaagga tattggcttg cattagtatt cacagaagtt
    15361 tattctattg ctggactatt tgcgggtatg tcaaatgtct tacaaacctt ttttcctatt
    15421 gcggcaatat ttaatatttc tggatactat ataactactg gagaaaaaat tatggggagt
    15481 atcataagtt tattactttg tgcctgcctt tctgcattta tacttaaagg gctaaaacac
    15541 aatgaaaaaa attgaaatag gtatatcaaa atgaaaaaga taaaaaaact gattattcta
    15601 attgtattct tgtttctttt tggcatgtta cactgttata ctgaatggaa attcaacaat
    15661 aacccaccta aaggggtaat agaaagaata aatgagtgga ctgataagat tggactggat
    15721 tagtaactta tgttaattat agtgaatgta atattagcaa gttattgttt cgtattttaa
    15781 tactataact ttaaatattt ggttttaatc ttccaaacga atataaaaaa actgtcacag
    15841 aggcaacata tttttgaata tttataaagt ggtagttttg attttcaaag ctaccgcctt
    15901 ttcttctata taagcaaact tatcctcaac aaagtgtaaa acatggtaca ttttaagaaa
    15961 aaagtaccac agacacaagc aataaaagaa acgccgagaa cctacaaaaa cgtagtgctt
    16021 atgcagattt acgacgcctt ctattttctc aaccgtctta atgaccttgt acaaaagaaa
    16081 tttttctaat attttttata atcttatctt gtgatgtttt ttatgtataa atgacttaat
    16141 aacaaatcca ataaaaatta tggatattgg tataattaga acgaggaatt tataatcatt
    16201 ataatgtgac attattctta cccaactctc agaaaaaata tatcctatac tgattaatga
    16261 agtattccat atggttattc ccacgataga tgatattaca aaagttataa tattttgccc
    16321 tgctatacct gctataaaag atatgtatgt tctacataaa gggattaatc tacatacaca
    16381 aacagaaata gaagagtatt tattaaaata atcttgagat gcaaatattc ctttctttgc
    16441 ctttggacat atctctatta ttttatttat gatagattta cctccccaat ttcccactgt
    16501 ataacaaaat atcgaaccaa gtatacctgc aattatactt aaaattaaaa ttgtcagaaa
    16561 tccaaaatga tttcttgcag caattgctcc acataaaggt aaaacaactt cgctaggtaa
    16621 tggaaaacaa gcatactcaa tcataattaa tacaaataca gagattaatc catattgtaa
    16681 aataaaacta ttgattacag acacttactt atctatctcc tttcttattt aaaggtttta
    16741 agggtacatg ttctaaggat tctcacccta tatttaaaga atcaatctct ttaaactatt
    16801 atatgtattt acacaaagca tttataacat actttaaaaa tagtacctta aaataagaaa
    16861 cttaataaat aaaaaaagat tacgtggcta cgtcctactc tcccaggcag tttcccacca
    16921 agtaccatca gcgctagaga gcttaacttc tgtgttcgga atgggaacag gtgtatcctc
    16981 tctgctattg taaccacata atctttatgc ttaatatttc
//
LOCUS       DAGSWI010000088        16766 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.088, whole genome shotgun sequence.
ACCESSION   DAGSWI010000088 DAGSWI010000000
VERSION     DAGSWI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16766
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.088"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            126..974
                     /locus_tag="MJF80_002596"
     CDS             126..974
                     /locus_tag="MJF80_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBZ0281249.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1038..2960
                     /locus_tag="MJF80_002597"
     CDS             1038..2960
                     /locus_tag="MJF80_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBZ0281250.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3042..3905
                     /gene="aroE"
                     /locus_tag="MJF80_002598"
     CDS             3042..3905
                     /gene="aroE"
                     /locus_tag="MJF80_002598"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBZ0281251.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4105..5322
                     /locus_tag="MJF80_002599"
     CDS             4105..5322
                     /locus_tag="MJF80_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0281252.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5558..7423
                     /locus_tag="MJF80_002600"
     CDS             5558..7423
                     /locus_tag="MJF80_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBZ0281253.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7601..8965)
                     /locus_tag="MJF80_002601"
     CDS             complement(7601..8965)
                     /locus_tag="MJF80_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0281254.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9144..10487)
                     /locus_tag="MJF80_002602"
     CDS             complement(9144..10487)
                     /locus_tag="MJF80_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBZ0281255.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10560..10661)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10990..11643
                     /locus_tag="MJF80_002603"
     CDS             10990..11643
                     /locus_tag="MJF80_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBZ0281256.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            11784..13055
                     /gene="brnQ"
                     /locus_tag="MJF80_002604"
     CDS             11784..13055
                     /gene="brnQ"
                     /locus_tag="MJF80_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBZ0281257.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            13257..14444
                     /locus_tag="MJF80_002605"
     CDS             13257..14444
                     /locus_tag="MJF80_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBZ0281258.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(14674..16698)
                     /locus_tag="MJF80_002606"
     CDS             complement(14674..16698)
                     /locus_tag="MJF80_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0281259.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKLLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT         6097 a         2075 c         2780 g         5814 t
ORIGIN      
        1 gataaataga aatcaaaaaa tacttttatt tttaaggtaa aataatacta ctagtaggat
       61 aaataaaaat ataaaatcaa aaatactatt aattataaag aaaataaagg gggcaaattt
      121 aagctatgaa taatcaaaaa aatcttaata ttccaattac aattagttct tatacattgg
      181 gaacagaagt ttcattccgt gatagagttc gtattgcaaa agaagctggg tttgatggta
      241 ttggattgcg tgctgaaaat tatattgatg caaaaaatgc aggtgttaca gatgaagaaa
      301 tgattgcaat tcttaatgaa tatgatatga aagttacaga agtagaatat ataactcaat
      361 ggggaagaga agaagataga acggaagctc aaagagaaaa agagcaaaat gtttatcata
      421 tggctcactt atttaatgtt aaacatataa attgtggatt attagagatg ataccagaag
      481 aacaaattat aactgcattg ggagaattat gtgacagagc aggtgaactt attataggtc
      541 ttgagtttat gccatatagt ggtgtaccag atttggctac tgcatggcgt gttgtgaaag
      601 gatgtaatcg tgaaaatgca atgcttatat tagatacttg gcactgggct agagcaaagc
      661 agacagcaga ggaccttgca cctgttccag cagataagat agtatcaatt caagtgtgtg
      721 atgttttaga aactccttat gaaaaactta gggatgaatc actacatgac cgtttggctc
      781 caggtgaagg atatggaaac acagaggaat ttgtgaaaat aataaaagaa cacaatatat
      841 caccaagagt cataggggca gaagtaatat ctgattctct aatatctaaa gggttatcca
      901 ctgctgctga aactgtattt aatgcaacta aaaaagtttt ggataaagca tggtctgaag
      961 tatctccaaa ataaacttat ataatataaa aatatacaga gtattataat ataaaacttg
     1021 aaaggaagga aaaccttatg aagattagtt ctatgtttac acctataaga attggttcaa
     1081 tgacagttcc aaatcgtttt gtagttcccc ctatgggaaa caattttgca aacacagatg
     1141 gtactttaag tgaaacttct aaggcttatt atcttgagcg tgctttaggt ggttttggat
     1201 taataactat agaatctagt gttgtagaca aaaaagcaaa aggaggtcct cgtaagcctt
     1261 gtttatatga tgatagcaca atagatagtt ttaaaaatgt aatagatgct tgtcatgatg
     1321 caggttcgaa ggtatctata caattacaac atgctggttc tgaaggaaat gaaaaagtag
     1381 caggacatcc attaaaagct gcttctgcaa tacctgcatc aaatggtcgt aatacaccac
     1441 tagcaataac aacagaagaa atatatgaac ttatagaatc ttatggagat gctgcattac
     1501 gtgcacaaaa agctggtgca gatgctgttg aagttcactg cgctcatgga tatctggtta
     1561 gtagttttat atcacaaaga acaaataagc gtgtagatga gtttggtgga tgttttgaaa
     1621 atagaatgag attacctcgt cttatcattg aaaatatacg taaaaaagta ggaaattctc
     1681 tagcaatatt atgtcgtata aacagcactg atgatgttcc aggaggaatt gatgtacacg
     1741 atagttcagt aatagcagca tatctagaag attgtggaat agatggactt catgtttcac
     1801 gtagtataca tatacatgat gaatatatgt gggctcctac gactttacat gctggattca
     1861 gtgcagaatt agttactgaa ataaaaaaag ctgttagtat accagtaata acagttggac
     1921 gttatacaga gccacagttt gctgagttaa tggtcagaca aggaagatgt gatttagtag
     1981 cattcggtag acaaagcttg gctgacccag aaatgcctaa taaagctaaa aatggaaaac
     2041 tagacgaaat gataccatgt attgcatgct tacaaggatg tgtaccaaat atgttccaag
     2101 gcaaacctat agcctgtctt gcaaatccta tattaggaca tgaagcagag cttaagccag
     2161 ctgaaattag caaagaagtt cttgttgttg gtggaggtgt tggaggtatg cttgcagcat
     2221 gggtatgtgc taaaagagga cataatgtaa ctttagttga aaaatctgaa gttcttggtg
     2281 ggcaaatgcg tttagctgca tatcctccag ggaaaggaga tattactaac ttagttagaa
     2341 gttatatttc aaaatgtaat caatatggtg taaaaatatg tactaataca gaagctacag
     2401 tagaacttat aaaggagaaa tcacctgatg tagttattat agctactgga gcaactccac
     2461 ttgtacttcc tataccagga atcaatgatt caggattaat tcatgctgta gaccttcttg
     2521 atggtaaaaa atcttgtggg aaaaaagtac ttgttgttgg aggtggaatg gttggatgtg
     2581 aagtggcagc cttccttgga gaacaagaac atgaggttac tgttattgag cttagagaag
     2641 aagttggtgc agatgtaatt tcagaacacc gtaaattcct tatgaatgat tttgaaaatt
     2701 ataatataca taccattact ggtgcaaaag tttctaaatt ttatgatgat ggtgtcgact
     2761 atttacttac agatggcaca gagcatgatt taaaaggttt tgattcagta gtacttgcta
     2821 tgggttctcg taattacgac ccattaagtg aagttataaa agaagttgta aaagaaactt
     2881 atattgttgg tgatgcagta aaagctcgta gggcattaga tgcaacaaaa gaagcttttg
     2941 aagtagcatt aaatatataa ataataaata aatttttctt tgtaaataac tgaataggtt
     3001 atttgcaaag aaaatgataa tattgagagg agattatagt tatggaaatt tcaggtagaa
     3061 caggattatt tgcattaata ggtacaccgg ttggtcactc taaatcacca gtgatgtata
     3121 attatagttt taagaaatta gatttagatt atagatattt agcctttgat atcacagtag
     3181 ataaggtaaa agaggcactt ttagcaataa aaacttttaa tataaaagga gccaatgtta
     3241 ctatgccatg taaatctgct gttacagaat atatggatga actttctcca gcagctcgta
     3301 taattggagc atgtaataca attgtaaatg acaatggaaa gttagttggt catataacag
     3361 atggagtagg ctatgttcgt aatttaaaag aaaatggggt agaagttaaa ggtaaaaaga
     3421 ttacaattat gggtgctggt ggtgcagcaa ctgctataca agtacaatgt gctttagatg
     3481 gagcaagaga aatatctatc tttaatccta aggatgattt ttataaaaga gcagagcaaa
     3541 cagttgagaa tataaaaaaa gatgtaccag aatgtgttgt taatctatat gatttagaag
     3601 atacaaacaa actttatgag gagatacaat caagtgatat attgacaaat gcaactctta
     3661 ttggtatgaa gccttatgac aatgaaacaa atataaaaga tactagtgtt ttaagaaaag
     3721 atttagtagt aacagatgtc gtttataatc ctaagaaaac aaaaatgatt gaagatgctg
     3781 aagctaatgg atgtaaggct attggtggat taggaatgtt attataccaa ggagcagaag
     3841 cttttaatct atatactgga ttagaaatgc ctgtagaaga agttaatgaa ttatgcttta
     3901 aatagataag agtaatatct taaacaatga aaaaacatac gtatttcaat atttttaata
     3961 ataaattaca aattgtcata gtgggttttg cctcatagtc cagttaaatt tgaagcttta
     4021 actgactata ctttaaatcc attatgggga tgagtttatt gtacacaaaa atgttaataa
     4081 attaacaaga atgggagaaa aattatgaac aataaaaaat attatcctac tgccatagcg
     4141 ttgtattttt catatttttt acttggtatt ggaatttcaa ttttaggaca atacaaacca
     4201 gaattttcat caatgtgggg ggctaaaaca ttatcagatg gaacattgga tgtaagtatt
     4261 gttttagctg ttattgctgc actaggatta gggagactta tatcttatcc atttgcagga
     4321 cctatttcag ataagtatgg aagaaaagta agtggtctta taggaaactt tctacatgca
     4381 attttctttg ttggaattgt attttcacca aatttttata tagcatatgt atttgcaata
     4441 attggagggg cagcaaattc attcttagat acctgtgtaa ctccttcttg tatggagata
     4501 tttgctagtt taggaacaat agcaaatatg tttactaaat ttacaatagc tttagcacaa
     4561 tttttgcttc cttttataat tggatttgtt gcagcaaatt caatatcatt taaagttatt
     4621 tttatagtaa cagcgattct tattgtagta gatgcctttt tgatagctgt gttaccattt
     4681 ccaccagcta ataatgttat tgataacaaa ggcaaaacag ttaaatcaga gaagatgaaa
     4741 tttacaccaa caagtatagc tcttgtttgt attggattta catgtacatc tacatttgta
     4801 ttatggttaa attgtaatca ggaattgggt aaattgtatg ggatggctga ccctacaaaa
     4861 attcaatcct tttactcaat gggagtaata tgtgcagtat taataacttc tttacttatt
     4921 aaaaaatata ttaaaccaat aagaatatta gttatatatc ctataatagc tttacttatg
     4981 ctattagttg tatattttgt tcaaactcca actatttgta tgttaggggg atttgtaata
     5041 ggttattcag cagcaggagg tgttcttcag ttaacaacgt caactgcaaa tgaaatgttt
     5101 ccaactaata aaggaaaaat aacatctata gttatgattg catctagtat agcaaactat
     5161 gttattttaa atatagcagg tattattaca aaatcagggg gagttaatgg accaaaatat
     5221 gttgtattgt ttaatgtggc aataacattt gttggaattt tacttgcatt gtttgtaaac
     5281 atgagatatg aaaaagaaaa agtgtatgat tatgatgtct aatattagta taatatatgc
     5341 atagagaagt ctaaatctag gatagatttt tattaaaagt aattaaaatt aataaggata
     5401 ttatttacag ttataagata gggtatttac ataattaata ataaggatat tactagaatt
     5461 tcaatttcta gtaatatttt tttgcgtata aatgtaaact tgatgaaata agtagtataa
     5521 tcaatataaa ttacatcatg taaaggagga taatttggtg gagaatgttt ttaagcgatt
     5581 gcaggaattt aatggttatg atggatacaa agaatcattt gaaatgaatt acctatgtat
     5641 atatgaaagt ataccgttaa gagaacaagt agagcttgca aataatctag tagatgaaat
     5701 tttaaatatg tataaaagtg aatctaatga aatttattta ttggagggta gtaattctaa
     5761 gtctttaatt tgttatttcg aaatttttat gaaaaagata aatactttgg taaaagaaat
     5821 gattattgat gagaaatggt tatataaatt aactaaagaa ttaatatata agagtaaaaa
     5881 agttgagtat gtaaaactag ggttagtttt aagtgagaaa tatctaaatg tagaaaactt
     5941 aagagaagtt gtagatacat tttctaaatc tggagaatac gtattttatc tgagtaatac
     6001 tattaaaaaa cttgaatttt acaatacata tttatttaat ttatctaaaa aggctacagg
     6061 ttcaataaaa gtatttgcta ttgttaatat ggaaaattta gattctaaaa taaattccta
     6121 tctaattgaa gatggttata aggatacaaa gtacgaacga ttattgatga actatattat
     6181 ttcaattgta gatttgaatg aatatcttga aaaacgagat ttagataaag aaaagataaa
     6241 taatttagca cgtcttatat gcaattactt gctaagtgta gaatttaaat atataggtaa
     6301 taaattagaa ttagtgaatc gatttttacc tactgtagta aattatggga ctaactttga
     6361 aagtttatat tcaattttcc taatagctat aaacgtctta aaagatgaaa atatagagtg
     6421 caataagatt gaatttgaaa aagaaataaa tggtatcttg ctcagtgaaa aatggaaaaa
     6481 tatatatttt gaagcattaa gagatgctag tggaaaaaca gaagatatta taaaaatgtc
     6541 agaaatttat gatgtaaact tatcttttga tgacttgttg ccatatctaa atagagatat
     6601 tagagatttt gaggtatatt ggcatatatc taaaaaggga acgacaagtt ctagattaaa
     6661 attattaaat ttttttgaag agacgtttaa aatagatgat ttaattggaa aaatgaaaga
     6721 tatagaaaaa gataaattga ctcaagaata ttatgatgat atgttatttt ttattgtgct
     6781 taaaggaagt aaatcactat atcctgaagg aaagaatata tctttaaaag gaatatttgg
     6841 aaatataaat gaagtaagaa aagaatccat aaatatctta aaaagataca gagagaaact
     6901 atctcttgaa gaattaaaaa ttgttaaaga agcgtatgaa aaagaaaaaa atgttatatt
     6961 gaaggatgaa ttaagaagag tattatatga aagtaataat ttaaaaaaag aatttgtaaa
     7021 tattgaaaaa ataaaagtag atgaacatgg taaggatatc tatttgactt caatagctgt
     7081 tgctggttca aggtttagaa atagagaata tcttgaaaaa gaattggaga aaagtaagat
     7141 atactattta actagagaga aagataatct atatgatgaa aaagctatta aaatagttgg
     7201 tgaaactgga tatgtgatag gctatgtccc aagaaaagaa aactatattt tgagcaattt
     7261 attagatgga ggaaaactgc tgtattgtag agtaactgaa tataatttat atgaagattg
     7321 tatctacgca aatgtgtact tgagttataa agatgtaatt gaaacagttg aaaactcttt
     7381 aaagatggtt ttagataaga gtagaataaa acttataaac taagacgttt atatattgct
     7441 tagatgatgt aatcctaact gattggttat tttttctgga attatggtgt attttatgag
     7501 caatgctaat gaatataaaa taaaaaaaca attgttttgt atatatgatg tatataaata
     7561 tatttatata ttcatattta caaaacaatt gaaatttatg ttattttata attttaaatt
     7621 tagatggaat tttaacttct gctttatctt ttattttaga agcttgtttt ttaactttat
     7681 ttaataattc agattcatct acaactaata cttttctttc cttcatcaac catttaccat
     7741 cacaaatagt agagtctaca ttttcactag tcatagaata gactatattt ccaattggat
     7801 tatgcattgg taaacaatga attgtatctg gatttagaat aatcatatca gctttttttc
     7861 ctacttcaag actaccaatt tctttttcta gtaaagcaca ttttgctcca tttatagtag
     7921 ccatttctaa gacttcttca gctggtacga cagttgggtt taaagttctt cctttatgta
     7981 ttagtgatgt aagatacatg tccctcataa ggtccattct attatttgaa ggagctccat
     8041 cagtaccaat tgaaacaggt attccttttt ctaacatttc aggaattgat gcaaacccta
     8101 gaaccacttt catagctgaa cctgggtcat gagaaacttt tacatcatgt aatctaaata
     8161 aatcaatttc cttatgagta agccaaactg tatgagcagc taaaagattt ggtcctaata
     8221 cacctagttt atcaaggtat tcaactgttc caactccatt gttttgcttt acaaatgaaa
     8281 tttcatcagc tatttcagca atatgcatat gtatacctgt attgtattta tcagctagtt
     8341 ctttagttct aacaagtaat tcttcagttg tattaaaaat agttcttaat gcaaaccata
     8401 ttttaattct ctcattagat gtattattaa acttatggaa taagtctaat tgagtgttga
     8461 tttcctcatc agtagttttt tgccatgcct caggaagacc atctcctgta tccataactg
     8521 atttagctaa acaggctcta agtccagttt tatcaacagc ttcaaccata gcatctacat
     8581 attgaccacc agattctaaa aatgtagtga caccagattt tatcaattca acacaacatg
     8641 cagtagatga aatcaaggaa tcttcataat tgaagctact ttcgtatggc caaattcttt
     8701 ctctaagcca agttaaaagt acaacatcat ctgcaagacc acgtcctaat tgttgagata
     8761 agtgaacatg agtattgact agccctggca ttagtatttt tcctttagca tcatatatct
     8821 ctgcatcttt atctacaata ttaatatcaa acttaccaac ctcttttata atattatttt
     8881 caataagtat gcttccattt tcatatacct ctctatctgc attcatagta attagatatg
     8941 cattttttat aagtaattgt gacataaaca ccactccttt tctatatatt tttatatcta
     9001 tatattatga taataaattt tctatacaaa gttaatagca aaatcaattt acatataaag
     9061 tgattttgct atttataaat ttaaaataaa taagatttta ttctatataa tttaaaatat
     9121 tgacctagaa ctatttaaaa atattaactt agaactaagt ttacaactgc aagtaaacct
     9181 ataatccaaa gtgtaatact gattttatca gtttctttag ctactacatg cattattata
     9241 tatgaaataa atccaaagca taaaccaata cttatactat aagttaaagg catcaataaa
     9301 actgtaacaa aagcaggtac agctattttg ctatcagcaa aatcaatctg ttttatgctt
     9361 tcaaacataa acacaccaac cattacaagt gcaggagctg cagcgtaagt aggaactata
     9421 ccaagtaatg gtgtaaatat aagtgtagct aagaataatg ctgctgttac tattgaagca
     9481 agtcctgttc ttgcacctgc tgcaatacca gaagcagatt ctccaaatgt tgtaacagta
     9541 ctagttccta agaatgagcc tattaaagta gaagaaacat ctgcatataa cattcttcct
     9601 aaatctttta tttcaccatt ctcatcttca agacccattt gtttacaaca agtaataaga
     9661 aatgctaatg aatcaaacat gtctataaac atgaaagaga atataggtcc aattaatgag
     9721 aatttcaatg cacctacaat atctaattta aaagcaactg gtgctatact aggaggtgtt
     9781 gagattattg ctgttggcaa ctccacaaat ccaaatataa cagatattat tgttgcaact
     9841 actatactta tcaatattcc acctttaact tttttatatt ctagtattgc cattaagata
     9901 agagcaaaaa gggctattaa aactgttggt gtaaaatctg caagtgaaac tatagtatct
     9961 ggattagcta ttataacacc catatttttt aaaccaacaa aagcaaggaa aagacctata
    10021 cctgctgaag atgctactgc caattctcta ggaattgcgt ctgcaattgt ctttcgaaga
    10081 cctgttactg cgagtgtaaa gaagacacat ccagatagaa aaactacccc aagacctgtt
    10141 tgccatggga ttcctgcacc tataaccaaa gagtatgtga aaaatgcatt taatcccata
    10201 ccaggagcta gagcaaatgg tgcatttcct aaaatcgcaa atataagtgt accaatggct
    10261 gtagctagta tggtaactgt tataagggcg gctttatcca ttccagcttc agataatatt
    10321 gctggattta cgaaaactat gtatgccatt gttaagaatg tagtaactcc acctattact
    10381 tctgttttaa ggctagagcc acgttcgcta aatttaaaca ttttgtctaa aaagcttaat
    10441 gatgaattat ttccacctgg ttgtacattg ttagtgtttg gtaccatttt tacctccaaa
    10501 aaactttaag atataaacaa aaaaatatat attcatatag atagaataga acatatattc
    10561 agagttgaca ttctattcat agaaaaccca tttaaggtga gtttgtagaa actattagac
    10621 cgtattacta atattatatg aacattttct atttgttttt ctgttgtatt atatcgtaat
    10681 attgtattat ttacaataaa tataataaaa ttattcgttt ttttgtcgat tttataaaaa
    10741 attaaggtta tttattgaaa aaaattactt tttccaataa ttgaatatgt atgagtataa
    10801 tgtcaattat tctaacttgt tttatactat taatggaagt ttattataaa tgagtttgtg
    10861 atataattat aaaagtactg aatacatgga tatttcgtaa aaacttttta atatatcaaa
    10921 tatgtatatt aagttaatat attaaaaaaa ataatatcaa aaataatata atttatagtg
    10981 gaggggaaaa tgactaaaat tattaaagtg caaaaaccta aatttacagg agaattagaa
    11041 attattgaaa atcttgaaga tatacttgaa gatatattta atgatgaaaa aatatttaat
    11101 aaaattatag aaaatgaagt tataagtgat ttagattctg taagagttag ctttgaatct
    11161 tgtatattta gaaatgtgtc ttttgaaaat tgtaatttaa aaaagattga tttacttgat
    11221 gtggtatttg aaaactgcaa tttatcaaat acattttttg atggaggaag tatatacaga
    11281 gttgaattta aaaatagtaa attgatagga gttagatttg atgattgtat acttaaaaac
    11341 gttttgttca aggatgtttt aggtaagtat tctaactttt cattctctaa atttaagata
    11401 gtttctatct tagattcaaa ttttgaaaat tctgtatttc aagaagtaaa aggtgacaat
    11461 ctagtatttg agtatacaaa tttaaaaaaa gcaaacttta atggaactaa actttctggc
    11521 atagatttta ctactaataa tattgaggga attgagattg gtatagatga tattagagga
    11581 gcaatttttg atgtgtcaca agcaattgat ttaacgaaat tgatgggaat tatcataaaa
    11641 tgaaaaaaat attcataaaa taaattggga aaacgttaac aatgtgtgaa tttttaccag
    11701 attgacattt ttctgaaaat aaagtaagat tatcttaata ttcaaaatat tttcaaaggg
    11761 ggtaatttag tgtgagtaag cagatgagag atatttttat agttggtttt gctttatttt
    11821 ctatgttctt tggtgcagga aatcttatgt ttcctccatt tttagggatg gaatccggca
    11881 aagactggtt aattcctctt attggatttg ttgttgcaga tgctggtata gcaatgcttg
    11941 ttattatagc aacagcaaga tgtaagggaa gcatggatga tgtacttagg agagctggta
    12001 aagggttagc aagagtaatg agtgtagctg ctctagcatg tttagcactt cttgtaatac
    12061 caagaacttg cgcaacaaca tatgaaatgg gtattatgcc tatatttgga gaccatggtc
    12121 cagtagctaa agctgtattt tcaataatct tctttgggct aactttagca tttactatta
    12181 ggtcttctaa ggttatagac ataataggta agtatttaac tccagcgtta ttattagtat
    12241 tatttgtttt aatagtaaag ggtataattt ctccagcagg ccctatgagt cctgagcata
    12301 tgatagataa aaatctattt ggtgaaggta tatcacaagg ttatcaaaca atggacgctc
    12361 taggtgcagc atctatggca actataatgt tgatgtctat aatagctaaa ggatatacat
    12421 cagataaaga ccaaatagga atgactatta aagctggttt tgtagcctgt gtatttctag
    12481 cagtagtata tggtggtctt acttatttag gtgcaacagt ttcaacttta tatgatacta
    12541 gtatacctca agcatcttta ttagttgaaa taactagtat attgttatat ggtcaagtag
    12601 gaaaggtaat tttaggtgta attgtggcac ttgcctgtct gactacttct acaggtttaa
    12661 cagcatcaat atcaacttat tttgaaggaa taagtaaggc gagttataaa caactggtta
    12721 taggtatatg tgtagctagt atgcttatat caaatttagg tgttgattct ataatagcca
    12781 tatcagttcc aatcctacaa actatatatc ctgttttatt agcaatagtt gttatggagt
    12841 tggctggtaa acatataaag aatgataatg cttttaaagg tgcagcatat gttacattag
    12901 tgattagttt attatcagct ataaatggaa tgacaggagc tgttccattt atacaaagct
    12961 tgccacttgc aggtttgggc tttaactggg taataccagc tatattgggt ggaatagttg
    13021 gtaactttat aaaaagcaac aaacaagtag cataataatt tgtaattaat ttaattttat
    13081 aatatattaa tttttagtac tatgggtagg tttaatctaa acctaatcta cccatagtac
    13141 tttttagatt ggattataat agcatgtata taatttttta taatataatt tttctttgaa
    13201 gtatgataaa attagtaata gtgataagaa tgtacaaatt taggaggttt taattaatgc
    13261 ttagaaaaat aacagcattt atgctatcca tatgtttaat tattggactt acaggttgta
    13321 gtttacttgt aggcaagaaa gataaagaga aaattataga tgattttaaa gttgcagtgg
    13381 taactcaacc attatctgaa aataaagtac aatacaatat ggtggaagag atggcaaaag
    13441 aatatgagga agagaataaa attgataaag acaaagatgg tcaaactaaa gttaaacaaa
    13501 ctataaagca tgttgtattg ccagagaatt ttacttctaa tatagacagt gctattaata
    13561 agatagtaaa gttggctgac gataaagaag ttcaggctat tgtagtttca actgaccaag
    13621 cagggctttt acctgcttta caaaaggtaa aggaaaagcg tccagaaata ataactattt
    13681 ctgcccctat gggagatgat aaaaaccaat taagccaatt tgttgatgtg aatctagggg
    13741 ttagtgcaga ggaaagagga aaagtattag ctgaacgttc aaaagaaatg ggagcaaaag
    13801 cttttattca ttatgcctct acagatgatt tgaaagatgt taatatagcc aaaagattag
    13861 aaatgattaa agagacttgc aaaaatatag gattgccatt tgtacaagtt aatactccaa
    13921 atataaatac tgaagaagac aaaaacaaag ttaagcagtt tttaaatgaa gatattgaaa
    13981 aacaagttaa aaaatatgga aaagatataa atgtatttgg tgtaaatgag tatatggatg
    14041 aagttatatt gactaaggct ttagaattaa agtatatagt tgcagagcaa agcaatccat
    14101 caccaataca aacatatcct agtgttatgg gtctaaaaat atctgaaaaa gatgctcaaa
    14161 attatgataa aataaacgac atgataagtg aaaaggctaa ggcttttggc atgtcaaata
    14221 gacttggtgg atatcctatg ccaatggatg cttttctacc atcagtagca atttatcttg
    14281 ctactgaaat ggtgaaacaa gatttaacac aagaagatgt atgtgaccca gattatttag
    14341 aggcgtttac agaattaaga tttggaatcg gttcagaatt tactccttta acagaggtac
    14401 tttataatta tcaatcagta attttaagcc aattaattta ttaatattaa ggacgtgtct
    14461 caaaatgaac tttttagttt atgaggcact ctttttctgt taaatcaaat atatttttat
    14521 ctttttaaca attaaaaaga agcttatcca tatgttgaga tagcttcgtt tttaatataa
    14581 ataagtttta ttattttata aaaaatttat agaaaaataa aaaattataa ttatatataa
    14641 attttgataa agtaaagatg aattactata ggtttaaatt atattatttt ctattatttt
    14701 tatgcctttc tcagttattg atgagccttt tcttccctta ctagaaatta agtatccatt
    14761 gcgtgtcaaa tattctagca tatttcttac ttcgtattct gtgacattat attccataaa
    14821 tttcattaac tttgtcctcc ccattggttt catgtgcaat gattttagga tagaaatgca
    14881 tatgtttttg tcatattggt ttaaatcatt gaatatggac atattacttg acatatgatt
    14941 attatttaat tttgaagata tgttttgagg taaatcattt attgtaagat aattggaacc
    15001 catgtaagcc atatattcaa cacaatttct cagttctcta atattcccca tccaagtgta
    15061 tgaatttaaa gcttctaaaa gactcttatc gagttttatg tttttatagg gaagttcttt
    15121 taaaaatgat tctaaaagaa gaggtatatc ttctaatctc tttctaagag gaggtaaatc
    15181 taaagtgaac atacttatcc tataaaataa atccatccta aatttatttt ctataattaa
    15241 tttctctaaa tctttatttg tggcagctaa tattctcaca tcaattggaa taatacttgt
    15301 accaccaatt tttctaattt ctttttcttg taagactctt aaaagtttaa cctgtaaatc
    15361 atgtggcata tctcctatct catctaaaaa tatagtgcca gtatgagcta attcgaataa
    15421 tccttttttt ccaccttttt ttgctccagt aaaagaacca tcttcatatc caaatagttc
    15481 actttcaagt aattcacttg gtaatgaagc acaatttatt gctacaaaag gtttattttt
    15541 tctatttgaa tgattgtgta ttgactgagt aaaggcttct ttacctgttc ctgtttcacc
    15601 tgttattaat ataggattat ctgtaagagc catttttttt gcctttttaa tacaatcttt
    15661 tataatagaa ctatttccaa taatattatt aaatttatat ttagctacat aacctctttt
    15721 gacagaatca cttcttattt tttgttctaa tatttctata ttgtttacat cttttaatgt
    15781 aattaagcaa ccctctatat ttttataaag ataaaatggt tttttagaaa gtatgaattt
    15841 tttatctatt tcgtctatgt atataatcat attatctata tttaagttct ttgtaatttt
    15901 atctaagaaa ctctttggca tatattccat taaggaaggt gattttataa gtttttcatc
    15961 tagttccaac atattaagta aagacttatt tacaaaagta accttattaa aggtattgaa
    16021 aaatattaga ccatcaccta tttcatcaat ggtcatgtaa agtatagttt ttaatttaga
    16081 tatattatct aatgatgtat ttaagaaatc atgacttagt gtctccttgg acaatttgta
    16141 taatttttct ataaatttct cattttttag ttttaatact actaataatg acatatatgt
    16201 atctaatgag atttttctcc aacctaaatc aacaatattt tttatgtatt ttggtataaa
    16261 tctaatctga cctggtgtta tagctgtatc taaatcatat atttcatcac aatctggata
    16321 tgtcggtaca aaatctatat gatttatacc aattttatat agataagtag ccatctcaaa
    16381 tgctgaagtt ttattggttg tcataagtaa caattttgta cctggaggca gtttcatcat
    16441 tttttcatat ccaagttttg aaagtgcttt cgttccatga ataatttcaa tatcttcctt
    16501 aaaatgtttt attaaaaaat tactagttaa aggtgataga gaaagcacta catctccctc
    16561 taaattttct tttattccat caagtatatt ttgtgtttct atgattattt cgtctccaaa
    16621 aaagtttgtt aattcttctt tgtagtggtc actattcaat ttatcatatg ttataaggat
    16681 taacttcttt ttatccattt tatttctcct aacaaaatag tatttatttt taaagtattt
    16741 aaagtataat gcaagttata ttatac
//
LOCUS       DAGSWI010000089        16761 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.089, whole genome shotgun sequence.
ACCESSION   DAGSWI010000089 DAGSWI010000000
VERSION     DAGSWI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.089"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            228..896
                     /locus_tag="MJF80_002607"
     CDS             228..896
                     /locus_tag="MJF80_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281260.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            898..1383
                     /locus_tag="MJF80_002608"
     CDS             898..1383
                     /locus_tag="MJF80_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBZ0281261.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1444..2565
                     /locus_tag="MJF80_002609"
     CDS             1444..2565
                     /locus_tag="MJF80_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HBZ0281262.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2846..4036
                     /locus_tag="MJF80_002610"
     CDS             2846..4036
                     /locus_tag="MJF80_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBZ0281263.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIANNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4170..4425
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4484..5485
                     /locus_tag="MJF80_002611"
     CDS             4484..5485
                     /locus_tag="MJF80_002611"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBZ0281264.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5589..5978
                     /locus_tag="MJF80_002612"
     CDS             5589..5978
                     /locus_tag="MJF80_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBZ0281265.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            6139..7347
                     /locus_tag="MJF80_002613"
     CDS             6139..7347
                     /locus_tag="MJF80_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBZ0281266.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7433..8509)
                     /locus_tag="MJF80_002614"
     CDS             complement(7433..8509)
                     /locus_tag="MJF80_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBZ0281267.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDVYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8738..9265
                     /gene="hpt"
                     /locus_tag="MJF80_002615"
     CDS             8738..9265
                     /gene="hpt"
                     /locus_tag="MJF80_002615"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBZ0281268.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9354..10286
                     /locus_tag="MJF80_002616"
     CDS             9354..10286
                     /locus_tag="MJF80_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBZ0281269.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10348..11574
                     /locus_tag="MJF80_002617"
     CDS             10348..11574
                     /locus_tag="MJF80_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBZ0281270.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11658..11879
                     /locus_tag="MJF80_002618"
     CDS             11658..11879
                     /locus_tag="MJF80_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBZ0281271.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            12068..12238
                     /locus_tag="MJF80_002619"
     CDS             12068..12238
                     /locus_tag="MJF80_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281272.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12331..12792)
                     /locus_tag="MJF80_002620"
     CDS             complement(12331..12792)
                     /locus_tag="MJF80_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281273.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKV"
     gene            12976..13443
                     /locus_tag="MJF80_002621"
     CDS             12976..13443
                     /locus_tag="MJF80_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281274.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            13486..15249
                     /locus_tag="MJF80_002622"
     CDS             13486..15249
                     /locus_tag="MJF80_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBZ0281275.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            15257..15865
                     /locus_tag="MJF80_002623"
     CDS             15257..15865
                     /locus_tag="MJF80_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBZ0281276.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            15937..16506
                     /locus_tag="MJF80_002624"
     CDS             15937..16506
                     /locus_tag="MJF80_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBZ0281277.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         6567 a         1694 c         2950 g         5550 t
ORIGIN      
        1 gataaggaaa atagcttttt atattgtaat gtatatttta aataattaat aaaaaaatat
       61 tatattttta aacttgaaaa gtaagtaatt ataagctatt taatcactgg aataaattta
      121 caataaaaaa ataaatacaa aggctttatc attaaggtat tggcataagt tttgcttatt
      181 aaacaagtat actaataaaa ttcatttaaa caggagggag aatttatatg agtaaattag
      241 aatcaacaac agaaaaagtt gaggtaaaat ggtatgggta tgtagcatta atattgggag
      301 cattattttt ttcaggaata tttaaagatg caccaggacc attaaaagta ttggatttta
      361 acaatgtatt aggttctttt ggaacattgg gaacaattaa tgatggtgta ggtacattag
      421 ctgcaaactt tagaggtgat ggaggtactg gccctcgaga tggatggcta tatgcattga
      481 cattaatacc atcagttatg tttgcacttg gagtagttag agttatagac cacttagatg
      541 gaatgaaggc agctcaaaag cttcttagtc cactattaaa gccattatta gggttacctg
      601 gatttgcagg actaactttg atagctagtt tacagtctac agatgcagct gcatctatga
      661 ctaaagaact taaagatgat gggtatatag atgaaaaaca aaaagctgta ttttgtgcat
      721 ttcaattttc aggtgcgtct gcaattacga atttctttgc ttctggagca gcactatttc
      781 catttatagg agatgtacca atatttatac cacttgcttt aatattaatt atgaagtttg
      841 ttggggcgaa cttacttaga ttatatttaa ataaatttga caagggggaa gcttaacatg
      901 agtgaggtag caaaaaaaga agtaaaagtt aaaaaaggaa atgtgataga tgcgtttatt
      961 ggaggagcta gaaatggatt ccaaataagt acaaattcaa tggctccaaa tgtcatcttc
     1021 ggttttgcta taatttctgt gttcaactta acaggtcttt tggatgtaat agggacaata
     1081 tttacacctg taatgagtat ctttggactt ccaggtgttg cagcaacagc tataatgaca
     1141 atatttatat caatgggagg agcatctgga gtaatcgcag gtttatttac agcaggtcaa
     1201 ataagtcaag cacatgttgc aattctattg ccaatgttat tgctttcagg agcgcagata
     1261 caatttatgg ggagactttt aggaacagct gagttaaaaa ctaaatatta tccacatatg
     1321 tttgttatag caactttaaa tggttgtatt gcaatgctta taatgaatta tttcgtagta
     1381 taggtaatta taataaaaat ttaatataaa tggaaagttt aaattttaag gaggagctag
     1441 attatgggaa ctaacaaaga ttatattgag tatgtacata aagtgtatga ttatctacac
     1501 actataccag aaaaaggatt tgaagaatat aagacatcag agtttgtttt aaaagagctt
     1561 gaaaaaatgg ggtttgaaaa tattaataaa ggagttgcag gaactggtat tatagtaact
     1621 atagattcta aaaaagaagg accagtattt gctataagag cagatatgga tgcattggaa
     1681 tttatcatag atggagaaaa gaaaatgata catgcttgtg gtcatgatgg tcatacttct
     1741 atgcttttaa cagctgcaaa gttaataaaa gagagaaatt tagtaaaaag aggtaagtta
     1801 aaaatacttt ttcaaccagg tgaagaaaaa ttatatggag cattaagagt aattgattct
     1861 ggtttgttgg atgatgtaga agaaatggta gggatgcatt taagacctgg acaagattgt
     1921 aggttgggaa aggcgatacc tgcaatgtac cattgtgcta gctgtataat ggaatttacg
     1981 attaaaggta tcacttgtca tggttctaaa ccacatttag gagtaaatgc agtagatgct
     2041 gtctgtttag cagtgaatgc aataaatgca ataagagtaa atccagctat accaaattct
     2101 ataaaagtaa caaatatatc agttaaggga gaaacttata ataatatacc agatgaagca
     2161 tttatggctt tagatataag ggcagaaagt aatgatataa tagaagaatt aaaagaaaaa
     2221 gcaatacatg ctgtaaaaac tgttgtaagt tctataaatg cagaagctgt actaatttcc
     2281 aaagatggag taccagcagc agaatttgat gaagaacttg taaacttggc tgaagaaact
     2341 ataaaagatg tattgggagc tgatgcatct ataggtatat ttaaaaattc tggtggagaa
     2401 gacttccatt attatacaca aaaactaaaa tgtaaaacta catatattgg attaggagct
     2461 gatgctacac caggatttca taatccaaat gtaacatttg atacgaaagc tttagaatat
     2521 ggtgtagata tatggtgtag attggttgaa aagagattgg gataacatcc aaactaataa
     2581 ttctacattt aaaattataa ttatagaagt tatagaaaaa gattgcttat gttaattaga
     2641 aaataattaa tatgagcaat cttattttga tataatgaaa ctgattttgg aatattgaaa
     2701 aaatatgatg gaaaagtaat tatattttaa atctattgga caaaaaagtg atattattat
     2761 aatattaaga attgtaaaat aataccaaag tgcatctagt aatagactta acaattataa
     2821 aaattttatt cttaggaggg aaaaaatgag taagtataat tttgacagag taatagatag
     2881 agtaggaaca gattgtgtaa aatgggattt tagaactaat tgttcaacaa aagcacaaaa
     2941 agatggtttg cctttttgga ttgctgatat ggattttgaa tgtgcagaac caataataga
     3001 agcattacgt aaaaaagttg aacataaaat atttggatat agttcaaatg atagtgataa
     3061 atactttaat gcagtttgtg attggtataa aaggagattt aactgggaaa taaatagaaa
     3121 agatattatt tttagtcctg gtgttgtacc tgctgttgct attttagtaa gaatattaac
     3181 taattctaat gaaggtgtaa tagtccaaaa accagtgtat tacccatttg aagctaagat
     3241 aaagagcaat aatagggacg ttgtaaacaa tcctctaata tatgagaatg gtacttatag
     3301 gatggattat gatgatttag aagaaaaagc taagtgtagc aacaataaag tactgatact
     3361 ttgtagtcct cacaaccctg ttggaagggt ttggagagaa gatgaattaa aaaaggttgt
     3421 agaaatatgt aaaaaatatg acctatggat aatagctgat gagatacatt ctgatttaat
     3481 tagaaaagga tttaaacata ctcctttaca gtcattatgc ccagagtaca aggataaaat
     3541 agttacatgt atagctccaa gtaaaacatt taatctggca ggaatgcagt tgtctaatat
     3601 aatagcgaat aatgatgaac taaaaaagaa atatcaagaa gaggttacag ctgttggagt
     3661 tgcaacttca ccaaatccct ttgctattgt agctacaata gcagcatata atgaaagtga
     3721 agactggctt aatgaattaa atgattactt agataataat atacaattta ttgatgaata
     3781 cttaaaagaa aacctaccaa aagtaaaact tgtttatcca gaaggaacat atctagcatg
     3841 gttagatttt actgcttatg gattaaatga agtagaactt gaggatttaa tgtttaaaaa
     3901 agctaatgta ttatttgatg aaggttatat atttggaaaa gaaggaatag gatttgaaag
     3961 aataaatgta gcttgtccac agtctttatt aaaagaatgt atggacagat taaagactat
     4021 atttgaaaat ttataaagta taggtaaaaa tttataaagt gtttaaaaat aaaaaaataa
     4081 taaacttgac aaattttaac taatcactta tcatactatt atgaataaat agaaaacata
     4141 agagagctaa attttattga attaaactct taaagacttt gaaaggaaga gtagttatag
     4201 aatgtaatta aagcgagttg gtgatggtgt gagaccaata tgaagtctat aatgaagaac
     4261 atcctggagt tgctaaccga aattttaaaa gagtaggctt agtcggaacc aaaccgttat
     4321 catattgggg tgtatcgagt aatatctgta cattgtgagc tgggtttgaa tttatttcaa
     4381 actaatcagg gtggtaccgc ggaaaagcaa cctttcgtcc cttttatata gggatgaagg
     4441 gtttttttaa tggaaaaata tataaatata gggagtgaat gatatgagca caataatacc
     4501 aaaggattat aaaagtagtt tgaacgtaat tgatacacaa atagctataa aaaaattaaa
     4561 agattttttt gaaatgagac taagtaatga attaaatctt ataagagttt cttctccgtt
     4621 atttgtattg ccagaaacag gtgcaaatga taatttaaat ggagaaaagg ctgtaagctt
     4681 tgatatacca tttatgaata aaaatgcaga aattgttcaa tcacttgcaa aatggaaaag
     4741 attagccctt aaaaaatatg ggtttgaagt tggtagtggt ttatatacag atatgaatgc
     4801 aataagaaaa aatgaagaat tagacaatat acattctctt tatgtagacc aatgggattg
     4861 ggaattggtt atagataaaa gtagtagaaa tgaaaagact ctaaaagatg ttgtaaaaag
     4921 attgtatgga gtatttaaag atactgaaat ttttgtatgt agtatgtatg aaggtataaa
     4981 agaaatatta ccagaagaaa taacttttat aacatcacaa gagttagaag atatgtatcc
     5041 agaattatca ccaaaggaaa gagaagataa aattgtaaaa gagaagaaag cagtatttat
     5101 aactcaaata ggaaaaacat taatatcagg agaaaaacat gatggaagaa gtcctgatta
     5161 tgatgattgg gaattgaatg gggatatatt attttacaat cctgttttag attcagcatt
     5221 agaattatct tcaatgggaa ttagagttga tgaagaatct cttgatagac aattgaaact
     5281 tgctggatgt gaagagagaa aagagtttga ctatcataag atgcttttaa atggcgaact
     5341 tccatataca attggtggag gaattggtca gtcaagaata tgtatgtatt tcttacaaaa
     5401 agctcatata ggagaagtac aagttggagt atggccacag gatatgattc aaaattgtag
     5461 tagtgcagga attgaattat tataatttaa atataaacta attgataaaa tttatatcta
     5521 atatgttcat caaattaatt atatcggtta taatgtagaa taacctataa ttataagagg
     5581 tgataaatat ggagttttta aaaataatca ttaatatagt tttagatatt ttaaaaaaaa
     5641 ttttggtcag atttaaaaat gctaaatttg gtttggtttt tgtatttgat ttacttaaac
     5701 tacctgattt tatgacagat aaaagaatca atatagttga taaaataaag gtaatttctg
     5761 tattaatttt tactatatcc tactttgtat ctggggttga tataattcca gagatgattg
     5821 caggggcatt tggttttata gatgatgcta tagttttaat ttggagtata ggtatagtga
     5881 atgaagaaat taataagtat agagttatcg caaaaaaaga taaacattca aatataattg
     5941 aaaatgtaga attttctata aaagatgaag aagaataaca atttatatat gttattcttt
     6001 tttttattaa caaaaaatgg ttaaattgtt gacggagagg taaaactcta ttatcataat
     6061 aagtggatat ccaatattta actaagtatt caatgtgcaa gaatgcttaa tttaaaaaag
     6121 ttatggaggg taaatattat gaagaaaaaa ataggtttag tgcccaaact aatcatcggt
     6181 atcatagtcg gtattttaat cggaagttat gcaccagaaa taatagtaca aatattagta
     6241 acagttagta ctttattctc agcattccta aaatttgtta taccatttat aattataggt
     6301 tttgtaacag caggtatagc tgatttagca acaggtgcag gaaagttatt ggggataact
     6361 acgggaatag catatggttc taccttaata gcaggattat tatcatttgc agtatctaca
     6421 ttaatcttcc caaattttat tgatgcaagt gttgcaagtc aaattggaga cccagaggca
     6481 ggaatgttag ctccaatatt cacaattcca ttagagccta tggtagatgt aacagctgca
     6541 atcgtatttg catttgtaat gggacttgga atatcagctt taagaaatca tggaaaagga
     6601 gagactttat ttaatttctt ccaagaattt caaggaatcg taacgaaaac gttgtcaaca
     6661 gtgattatac ctttattacc tttatacata gcaggaactt ttgcaaatat aactatagca
     6721 ggggaagtat ggacaatatt aggggtattc tggaaagtat acttagtagt aataccttta
     6781 cattttgtat atatggcatg tcaattcaca gcagcaggtg tattttcagg aaaaaatcca
     6841 gtaagaatgt taaaaaatca agtacctgga tatttaacag ctataggaac tcaatcttca
     6901 gcagcaacta taccagttaa tgttggttgt gcagaaaaaa atggagtaag taagcaaata
     6961 agagaatttg ttgtacctct ttgtgcaaca atacatttat caggtagtat gatatcaata
     7021 acaggattta caactgcagt tttaatgatg aatggtatgc cacatggaat aggtgtatta
     7081 ttcccttata ttgcaatgtt aggtatagca atggttgcag caccaggagc accaggtggg
     7141 gctataatga gtgcacttcc tttcctacca atggttggaa taccatcaga tggtgggctt
     7201 gcaagtttaa tgatagcttt atacttaact caagacagtt ttggtacagc atgtaatgta
     7261 tcaggagata atgcaatagc agtagtagta gataaaataa atgatggaat gagtaaaaaa
     7321 ggaaacaaag aagagcaaag tgcttagttt tttaataatc aaaaatgagt tatctcaaat
     7381 agagatagct cattttttat ggtataaaaa attataaaat aaagtgattt ttttatttaa
     7441 aaattgcttt aagagatgat attaataatt tattttcatt aggttttaat atacatactc
     7501 taaagaaata ctcatctaga ccttcaaatg aacaacaatc tcttattatt attttttgtg
     7561 ggagcagttg ctctcgaaga gatttagcag tcaattcctt tgtcttgatt ttacacaata
     7621 tgaaattacc tttagtattg tatacatcta agctttttat attcttaagc tctttaagca
     7681 ggtaatctct ttcagtgttc attaaggaaa tagtatttga tataaaatct aaatcactaa
     7741 acatgatttc acccatttta gaagctatta tgtttatatt ccataaatct agatttttat
     7801 ttatttcatt tttgacatta gtgttagata taagaccata tccaagcctt attccaggcg
     7861 ttgaaaaaaa ttttgatgta cctcttataa cgaataaatt agaataatca tcaactaatt
     7921 gagtacaaga gtatgtatct gtatcagtaa actctacata agtctcatca atcattaaaa
     7981 atgagtctgt attttttaaa atttctctca cttctacttt agtaaatgca aatccagttg
     8041 gattactagg attacagatt actaccaaat cataatcttt agcattgata gtttttataa
     8101 gattttctaa atttatatga aaattatctt cttcttttgc aaagtatttt tctatagaac
     8161 aattaatttt agataactct ttttcatact ctgaataggc tggagagagt aaaagagcct
     8221 gtttagggtt aattgtatgt ataaatgatg aaattagttc tgtagctcca ctgccaagta
     8281 ctatattatc tatagaacat ttgcagtaat tggatataga agtttttaag tctatgtaat
     8341 cagggtcagg atacatagat accatatcta tattgtttac aatgtattgt ttggctaaac
     8401 ttgatgtacc aaaaggattt atatttgagc taaaatccat aaattcttcc tttgaaaagc
     8461 catacttact agataaacta tataaattag ctccatggtt tgtgctcata aaagtacctc
     8521 caaaaataaa attatataat cgataaaaat aactgattaa tatatgtaca tatagattat
     8581 aatacaaagg aatcgttatt ccaaattttt aatgtaaaaa gcaaaaaagt atggataagc
     8641 tgttaattta ggtttaaaaa tgatgaaaaa gattttaatt gatattttgg tatgctataa
     8701 tatataagtt aatagaagga aagcgggagg atacaaaatg tacaaagtta caggaaaaat
     8761 gttgacagaa gaacaaataa aagaaaaagt ttatgaatta ggaaaaaaga tagaagaaga
     8821 ctttaaaggc gaagatttac tagtggtagg tatacttaaa ggagctagtg tatttgtttc
     8881 agatttaata agatgtatag atttggatgt aaatatagat tttatgagtg ttacaagtta
     8941 tggaaactca acagagtctt ctggaacagt taaaatatta aaagatttag atgtagatat
     9001 tgaaggaaag aatgtgttga ttgtagagga tataatagat tctggtttaa cattaagtaa
     9061 tctagttgca gctttaaaaa ctagaaatcc aaaatcttta aaactatgta ctctacttga
     9121 taaacctcag agaagaaaag ctaatatacc tgtagactat gttggatttg ttatagaaga
     9181 caaatttatt gttggttatg gaatagacta tgctgaaaag tatagaaatt taccttatat
     9241 aggtatagtt gaggatgtag aatagattta aatgtgaatc aaaagcacta atttctaagg
     9301 gagttagtgc tttttagtta attatataaa attttagtaa aggagacatc tatatggata
     9361 ttcagttaaa tatattatta tttttccaaa atttaagaaa tccaattttg aactttatat
     9421 ttcttgtctt tacaataagc acagaagcac cgttattaat attaattaca acaattatat
     9481 actggtgtat aaataaaaaa tgtggacaga aaatactgtt tgctataatt ggaaattttg
     9541 ttgtaaattt aggcatcaaa gagtttgtaa aagctcccag accaatagga ataaaaggtc
     9601 ttgaatcatt gagagtttct actgctggtg gttattcttt tccaagtgct catactcaaa
     9661 ctgcaacaac attttgggtt agtattatga ctatatttaa aaagagatat cttcatttaa
     9721 taggtgcagt tatggtttta ggagtaggat tgtctaggct gtatttagct gttcattggc
     9781 caatagatgt gatagcgggt tggatattag gaatattttt tacagttgta tttgtaaaaa
     9841 tatttgacta tattgatgat aataaaaatt atattttact tgtagtttta cttataccat
     9901 ttataatagt ggctatattt ctaaatagcc ctgaatattt tgaaaaattt ggtataatag
     9961 taggatttgt atttggatat atggttgagg atagatttgt taaatttaat acagataata
    10021 ataacaaaaa aataaatttt agcaataaaa ataaggggag taaaaatata attttttcaa
    10081 gaatatgtag gtttttggtt gggatagtaa caataggaat gttatatatt ggcattaagt
    10141 tgtttatttc aatgttaata gtcactttaa atataagtga ctctactatg agtataattt
    10201 ttatgaattt tattaagaat acagtagttg ttttttatgg aatagcagga gctccagcat
    10261 tatttaaact tttaaaatta aattgatttt aaggtataga gtcaatagtt taaattaaaa
    10321 aaattaaggg tagaaaagag tgattatttg aagaaggtag taataatagg tggtggacca
    10381 gcagggatga tagccgcatc aactgcatgt gagaaaggct atgatgtaac attaattgag
    10441 aaaaatcata aattaggaaa aaaacttgca ataacaggaa aaggtagatg taatattact
    10501 aacgcttgtg aaatagagga attaatagaa aatgtaccta caaatggaaa atttctttat
    10561 agtgcttttt atacttttac aaatgatgat gtaataagta tgtttaataa tcttggagta
    10621 aagactaaaa cagaaagagg aaaaagggtt tttccagaga gtgataaagc atttgatatt
    10681 gtaaatgctc ttgaaagaca acttaaaagt aagaaagtaa atatattatt aaattcgaaa
    10741 gtagaaaaaa taatatctaa aaataataaa attgaaaaag taattttaaa cgataaaaaa
    10801 gaaattaagt gtgattcagt tgtagtagca actggtggat taagttatcc acttactggt
    10861 tctactggtg atggatataa atttgcaatt tcacaggggc acacaattat agatacaaaa
    10921 ccatctttaa ttggtataga agtacaagaa agttttacaa aagatttaga aaaattatca
    10981 ttgagaaatg ttgaaataag ggtgtttaat agcaaacaaa agaaagttta tagtgatttt
    11041 ggagaacttg aatttactaa atttggttta gatggaccta taatcaagtc tgcaagttgt
    11101 agaatgaaag atacaaggaa agaaaattac actatattat tagatttaaa acctgcatta
    11161 gatgaggaaa aattggataa aagagttcaa aaagattttc agaagtatac aaataaaaaa
    11221 tttgaaaaag cattagatga tttacttcca aaaaaactaa tacctataat aataaactta
    11281 agtgaaatta atccaaatac ggttgttcac caaatttcta gagagcagag aaaaaactta
    11341 gtccatttat taaaaaattt aaagtttaca gtaaagagat acagacctat tgaagaagct
    11401 ataataactt ctggtggtgt aaaagtaaac gaaataaatt caagtacaat ggaatcaaaa
    11461 ttagtagaag gtttattttt tgctggagaa gtaatagata tagatgcata tacaggtgga
    11521 ttcaatttgc aaatagcttt ttctactgga tatttggcag gttttaattg ctaaaaatta
    11581 cctgaattaa aaatgtttaa aatcacataa tattatcagc aacacaaaat aaattaaaca
    11641 gaaggaaggt aatcaacatg gcaagtaaca ataacaacaa cagaacagta gtaccaggag
    11701 caaaagaggc tttaaaccaa atgaaattag aaatagctaa tgaacttggt atgagtaatt
    11761 atcaacaagt tgataaagga aaccttactg caagagaaaa tggttatgta ggtggataca
    11821 tgactaaaaa attagttgaa atggctgaac aacaaatggc tggaaaaagc aacagatagt
    11881 tataaataat tatttataac tattggctat tagtttagct agagctatct caaatataga
    11941 aagtaatttc taaattgaga tagctttttt ttattaaatt attaaataga tatattttta
    12001 tgtaataaat tttatgtatt tggataaaat aatacaaatg aatatgttac ataaggagtg
    12061 atgtaaaatg agtagtgtaa attttgagga tttaaaaaac aaatttatta atagtgactt
    12121 agatgaaaaa ataagaatat atacaacaac agaaggttta tcagtagaac aatttaaaga
    12181 gctactaaaa tattatccaa tacaacattt atctaaacta gaaaaagcac ttggatagca
    12241 gtatttaatc caaaattaaa aaggcaattt atttaatatt aaaaatattt tttattaaat
    12301 aaattgcctt tttataatag gtttaaatta ttatactttt gatatataat tgtagattct
    12361 ctcatttcca agttcaatag acccagtatt aataagagta actattcctt ctatatctct
    12421 agcagttaag tatttatatg gaattaataa attataacca gtattactta gtaagttttt
    12481 taattgggtt atttgagaga tattgaaaca ttttgcagtt ttagtagatt tttttcttaa
    12541 gttagataaa ttttcatttt gaatatttat taaagtttct atatagtttc tataagatat
    12601 cttaaagttt gtctcattga ctattttttc attaagtata ctactgataa aatcttgaga
    12661 caatatatta tctgcaaatt tatctgctgt taattttcca gatgttatgc ctttttgata
    12721 ttttctaaga atttttttag tatagcagta tattttattt ctcgtttcta aatcgagttg
    12781 ttctatttgc acaaaaccac ttccttctaa attataaaaa tatttattaa aaatctatac
    12841 ttaactaatc aagcttcaat aacttattta caataaatac taaataataa aatatttgtt
    12901 attttttatt attaccaagt ataaaaaata ttataattgt aaaacttaca aatatatgta
    12961 aaatatttag gagtgatgaa aatgttaaat gggataataa agctttttta ttctgaaatt
    13021 gtaaaaacaa agattaataa cttagattca agtggatatt ctttaagaga tgaatgtaaa
    13081 gaaatcagaa atataactct aaagatagat agtgtatttt ttaagctttt aaagttagct
    13141 tttggttcaa tattgcttat ggtagctttt aatttaaaca tggttttagg tataggagtg
    13201 tttttagttg gtgtagttta tatactatat aaaagaaata tggaagaaca agtgaaagag
    13261 catataaata atgtgaaaaa taatatcgac atctctaaaa taacttttat ggatgaaaaa
    13321 gggagaacag gtctaaatgc attaattaca ttacttctta taggattatt gacaggattt
    13381 aactggatat tagttgctag ttttgtcgtt gtttttgttt ttactataaa gaatgtatgt
    13441 taaaatacta ataaatagtt taatatatag ggggagaaat tgagtatgac taatggcagt
    13501 tataaaatga agaatttagt ggaaatatcc gctatggtga ctgaatctac agattttttt
    13561 aatataaaag atgacataat agaaaaaatg ttagaagttg ttcaccctac aaaggcatgt
    13621 gtgaatctat tttataaaaa tgattctaaa tatgcctact tagtatgttc gcaaacctta
    13681 gaatatgtac ctcaaatttt tccaataaat agtcttaaag gtgcaaaaat tgattttgac
    13741 acatatccag agtatataca tgaggctgta aatgaaaaaa agataatata tgtggaaaat
    13801 gtatttgaag attctagagc agaaggtgaa cgagatttag caaaagctga aggttatatt
    13861 gggagaatag tttttccttt tataataaat aacgtagttg ttggatttat gacttgtttt
    13921 ttaaaagagg atgattatat aacagaacaa gatatagatt ttatatcatc tgttgcatca
    13981 ctcttaagtt tatcaataga aataactaat aaaaataata atgtacaaat tttaattgat
    14041 aaactaagag gctcaataag tgccataaat gaagctacta aaaaactata ccttaataag
    14101 agtattgatg gctttttgga aaatttaagt aaacaagcgt gtaatattac taatagtaaa
    14161 gaagctttaa taattataaa tagtcatcat aataaggatg ctatagttag ttgttataat
    14221 gttgagcaaa aaaagaaaac aaatttatat cctatgattg atatgttttt aaagaaagag
    14281 tcattggggg ggtatctaaa taatttaaaa ccatgctcaa aaaagggaat aaatttggaa
    14341 agttatatat attacaaact tcaggataaa aatgatacta taggatgtat agtttgtgca
    14401 aatagcaaaa actatactag tgatgattta aatattctta gtatattgtc caaacaagtt
    14461 tctgttggta tgcaattata tgaacataat gaaaaggaag taaaacacaa agttcttgaa
    14521 aacgagttga acatattaaa taaacaacaa cagcttatta tggataaagg taaaatggat
    14581 tgtaatgatg agaaagaact ttatttttac cataaaccag caagggtagt aggtggagat
    14641 ttttattatg ctacaaaagt ggatgacgaa catatagttt atattgttgc tgatgtaatg
    14701 ggacatggaa tggtatcaaa ttatgttgtt gcaatgataa aaggtgcttt taaagtatta
    14761 tgcaatcaat acaatacacc aagagaaata atgacaaatt taaataggat gttatatgat
    14821 gaatttgaca agatgggagt atttacaact tgtttgatag gtattataga tactaaagaa
    14881 aatatgatta ctgtgtctaa tgcaggtcac tatagtcctg tagttgtaaa gaaagatggc
    14941 acaatagaaa ataatttaaa ttgtaaaaag ggcataccta ttggaataat ggaagatgct
    15001 acttatgaaa ataatactct ttcaatggaa gattatgcaa tggtatgtat gtacactgat
    15061 ggaattttag agataaaaaa ttcatcaaaa gaagagtatg gtataaaccg attagaaaac
    15121 tttctaaaag aaaattttag attaaatcaa cagttaatag tagaaaatct aaaattggat
    15181 ttaaagaact tctcatcaaa agataattat gatgatgata ttttaatagt tatgttaaag
    15241 gataggtaga ttgattatgg attcaaacga taaaaaagga cttaaaagtc cagagattat
    15301 aataataaat ggaagtccaa gaattaataa aaattgcagt tttatagcaa atgaagtaaa
    15361 agctatgcta gaagagtgtg gattaagttg taaaatattt aatatctaca atatggatat
    15421 tgaatattgc acagcatgtg gattttgtga aaaaacaggg tattgcaagt ttaatgatga
    15481 tatgaagcca ttatataaaa tgtttgatga ttcaaaagga actatagtaa taagtcctgt
    15541 acattttgat tcaatatcag ctaaattaaa gactgttgtt gatagaactc aagctatata
    15601 tgcaagtaaa tatgtattaa ataaaccttc aattgataga agtaagaaac ggataggaat
    15661 gtatattgca gtggggggga gtaaatcata tgattcacag tttaaaggtg gacagattgt
    15721 gatggatttt ttctttaaga gtataaatac taaactttta cacaatatat atttaaacaa
    15781 tacagatgat gtatcttata tagataatca agaatttaaa gatataatat ataaaaattt
    15841 aaagatatat attgattcaa tataatttga ttttgtaggt aatattatgt ataattgtat
    15901 tgcaataatt taaataatta aaaggaggga atttcaatga aattaccaat agcattatct
    15961 aataaacatg ttcatttgag tcaagctgat atagacgtat tatttggggc aggtcatgag
    16021 ttaactaaat ttaaaccatt atctcaacca gggcaatatg cctgtgaaga aaaagtggat
    16081 ttagtaggac caaaaggaac tttaaaagga gttagagtac taggaccagc tagagctcat
    16141 actcaagttg aaatatcttt agcagatggt ttcccattag gagtaaaagc accaataaaa
    16201 gaatctggtg atatagctgg aactccaggt gttaaattag taggacctgc tggagaagtt
    16261 gaaatgaaag aaggagttat agttgcttct agacatatac atatgagcaa tgaagatgct
    16321 gctaaatttg gtgtaactga taagcaaata gtaaaagtta aaacatctgg accaagagca
    16381 ttagtatttg aaaatgtatt agtaagagct agtgacagct ttaatctaga aatgcatgtt
    16441 gatatggaag agggaaatgc agcaggcgtt aaaaatggag atttagtaga attaatagtt
    16501 gattaatatt ataaatagac tttatgctag tagatatttt atttgctagc atatttttaa
    16561 atgtaataaa tttctatagg ataaatttaa tcgattagat taatttataa aataaagggg
    16621 tattataaaa aatgtaataa aaacttacaa aaatatgatg taatatggta aaattatata
    16681 tataaaaatt tacatgtaat aaggataagc attttacttt ttatactaat atgaacaaat
    16741 agataaaaaa gtttgaaaaa t
//
LOCUS       DAGSWI010000090        16660 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.090, whole genome shotgun sequence.
ACCESSION   DAGSWI010000090 DAGSWI010000000
VERSION     DAGSWI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.090"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(330..851)
                     /locus_tag="MJF80_002625"
     CDS             complement(330..851)
                     /locus_tag="MJF80_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBZ0281278.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKIKDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
     gene            1017..1583
                     /locus_tag="MJF80_002626"
     CDS             1017..1583
                     /locus_tag="MJF80_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBZ0281279.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(1800..3852)
                     /locus_tag="MJF80_002627"
                     /pseudo
     CDS             complement(1800..3852)
                     /locus_tag="MJF80_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(4233..7568)
                     /locus_tag="MJF80_002628"
     CDS             complement(4233..7568)
                     /locus_tag="MJF80_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBZ0281280.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYTNDSDLLLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTANYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLTSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKTETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRKLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(7618..8046)
                     /locus_tag="MJF80_002629"
     CDS             complement(7618..8046)
                     /locus_tag="MJF80_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281281.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDHLLTCEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(8036..8416)
                     /locus_tag="MJF80_002630"
     CDS             complement(8036..8416)
                     /locus_tag="MJF80_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBZ0281282.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTKALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(8449..8757)
                     /locus_tag="MJF80_002631"
     CDS             complement(8449..8757)
                     /locus_tag="MJF80_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281283.1"
                     /translation="MNQIELDLYHDMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(8790..9890)
                     /locus_tag="MJF80_002632"
     CDS             complement(8790..9890)
                     /locus_tag="MJF80_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBZ0281284.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVKVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGNVITNSKDWDTELLGEISNLKAGKNIKAKDIYEK
                     NSHELYPCYGGNGLRGYVKMYSHKGTFNLIGRQGALCGNVKYVNGKFYATEHAVVVQP
                     KVDINSYWLYFTLKELDLNRLSTGAAQPGLTVGKLNEVEIPKVPVYLQNKFVDFVRQI
                     DKLKSRMEDSLKELENNFNSLMQKAFKGELFN"
     gene            complement(9894..11357)
                     /locus_tag="MJF80_002633"
     CDS             complement(9894..11357)
                     /locus_tag="MJF80_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HBZ0281285.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            complement(11867..12736)
                     /gene="gatY"
                     /locus_tag="MJF80_002634"
     CDS             complement(11867..12736)
                     /gene="gatY"
                     /locus_tag="MJF80_002634"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBZ0281286.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(12756..14672)
                     /locus_tag="MJF80_002635"
     CDS             complement(12756..14672)
                     /locus_tag="MJF80_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBZ0281287.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSGVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(14675..15595)
                     /locus_tag="MJF80_002636"
     CDS             complement(14675..15595)
                     /locus_tag="MJF80_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBZ0281288.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(15697..16449)
                     /locus_tag="MJF80_002637"
     CDS             complement(15697..16449)
                     /locus_tag="MJF80_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBZ0281289.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
BASE COUNT         5199 a         3059 c         1640 g         6762 t
ORIGIN      
        1 ataaaactaa aaaagatgtt gtctcaaaat atagataaat ttatttttca ttgctaaaat
       61 gattaaaata tatttgattt aatatacttg atttaatata aaaaaaagag tgtctcataa
      121 actaaaaagt tcacttttag acactccttt atatatatta tctacttctt aattcattta
      181 ttacctctct acattttctt tcaactagtg gatacatctt tataagtact aattactcca
      241 cttaaaaatg attcaggtac tgtccatcag tcatattttt ctgccaaata taatggtgtt
      301 tcataatgag acaataaata tttatatctc tacttatctt ctctaagctc gtcaaatcca
      361 actataggta tatttaaatt atattcttta agtagctttt ctagctcttc tataaaaata
      421 cctttcccat ccaccatttg tatatccttt attttatcca tcaattcatt gcaaccactt
      481 aactcaccaa accaacgttt agaactacat gtcttattat aaccacaact tggactgcca
      541 tctattgcaa tgatactttt tatatcataa tcatttttta aatattcatc aaactgcctt
      601 acatgaggta ttaacatttg cctacattgt tctctaaaaa acaaattgtc aaactgttct
      661 tttacatgtc cccacctatt tattccatac ataatcattt ctgggcatgg tagttgaatt
      721 attccatacc ctttatttat taaatttatc gctatatttt gtgctccctt aaattctgac
      781 aatccctcca ctttagaatt acaatttaat atacaatgac atactactac aatctctcta
      841 tttcttttca taataatctc cttagttatt cttaatttta atgatactac atattttgat
      901 aattatatta tttatttgtt ctatatttcc aatattctat atagaaatat acaattagta
      961 atcttttata atctatacta tgataaatgt atatattttt ttaggaggta ttataattgg
     1021 atactaaaaa agtcacattt tcttcaatgt gcatagttat taatattgtc ttaggtatgg
     1081 ttatatctat gataaatata ccattattat ttttagatac agttggtact gtacttgggg
     1141 ctgtcgtatt aggtcctttt tggggtgctc ttattggtgg gtgtacaaac ttagttttag
     1201 gtgttatatc aggtcctaca aatatacctt ttgcccttgt aaatattgtt cttggtctta
     1261 ttgttggata tatttctaag aaaaaaggtt ttggttataa agaagctatt attacaggag
     1321 taattttatc tattgtttgt cctttaattg gtactcctat ttcagtttta ttgtttggtg
     1381 gtttaagtgg aagtggtgct gatttattgg ttggtttttt agttcagtct ggtcagaaga
     1441 tatttactgc tgcgttcttt ccaaggattc ttagtaatat agtagacaaa cctttgtctt
     1501 gtattatggt ggtatatttt ataagtaaaa tgcctaaagg ttttataaaa caatatagta
     1561 aaaatacttc tagttcatat taataaatca tttattatgt aaggtttatt actctttaaa
     1621 atgctatata ctcattttta tataacattt ttattaataa tttaattaga tagtatttgt
     1681 attactttaa ttaaacgtaa cctcttgata taaatttgag ctataaatct atatcaagag
     1741 gtgctatttt atttttaata taattaatat tctattacgc aaaaaatgca tatatttcat
     1801 tattttatgg aaaatattac ttcatttaac tctatatcaa actcattaag taaaaatcta
     1861 attatattta atttagcctc tgtatttaaa tttgtttcta aatatatatt ttctgatatt
     1921 ttaatacctt ttcttaattt atatgcatct ctagacttaa tattatctat aaaatctctt
     1981 ttataagttt tgtctacaat actcagaatc caaatcatat aaagtaagag caacttgttc
     2041 ataaaattct gtccaatttt tagccttata tgactctcct aatatactaa atgatactat
     2101 actttcattt gtaaaattaa catcatcatc ctctaacgta aatatatttt cactgttact
     2161 tggaggttca tatgtaacac ttggatattt ccaaatttct acagctctat ctagtaatat
     2221 cttagttcta ttttttattt cttcttcatt ccacttatct atagataaaa tatatttatt
     2281 tagataaagc ctactatctt taaatccatc ttccatttct ttcttttctt caaatgtttt
     2341 attacttagt tttgaattat atcctgtcaa agttaaattt ccaattgtat gtaaatattt
     2401 atcatgtatc tccttatagt tatctcctag tgattccttc catttatttg ttaatgtctg
     2461 tggcattata tgctctatat ttagcatatt attattgatt aaattttcta aatcaacaat
     2521 ttctttatta ttataattct ctaacctctc aagtatatat aacttattct ttgacttagt
     2581 attatatata tctttagttt caaaagcatt tgaaaattca ctgtcagtag gaaatctttg
     2641 agatgatttc ttatttataa aaatatatct taatatatta gaatagtttt ctttataatc
     2701 actaaatttt ttaatttccc ttcctaagtt cataaatact ttattcagtg cattcggagg
     2761 tacatcacat actattcttc ttaatataaa acattctact aaattaaggg attctacaag
     2821 tactttttta tttattatac cttcattata atcatcaaat aattctaata taaacggata
     2881 tgatacaaat atttcaagtt tgtttatcct tttcaaaact ttgtttacat cattatcatc
     2941 agtacatgaa taagatatct ctctataata gttagaaaat ttaagtaaat cttttaataa
     3001 aatttctata tctataccat tatctgatac atacctttta aaactaaaat atactttatt
     3061 tttattcggt atacttctct cctttatagt caaatagtct cttataaaac tacttacgtt
     3121 gctacttgta tttttttcaa ttttatacca ataattatta taaaattcct cttgtaactt
     3181 agagcttcta cccattaaca caaagtttct tactctatca gcttcactta aatctaagcc
     3241 agttgaattt agactttcaa agattaattg agggtcatct tcaccatttt ttaactctat
     3301 ttctactata gttaatttct gtatagcatc aaacaaatca tctattgata tattatcttc
     3361 ttttattcta ttataaaaat ataaataatt tgaaactata ttagaatttc tatctgtatt
     3421 catttcttct tcaaatagtt catagtacgc ttttctatct tctgctattg gttttaactt
     3481 tatttttcca tctttatctc ttgagtgctt gtttataaga taacactcct taatctgttc
     3541 ttctgttgta ataattcctt ttgattcccc aaactctttt attatttgac acatggcaat
     3601 caataatatt gacacagtag tcactctttg ttgtccatct attattaaat attcttttcc
     3661 catactatat atagatacaa ctgaccctaa gaaatgagtt ctaaatccag aatctattat
     3721 atcctctaaa tcatcaaaca atcttttaca ttgttctttc ttccagttat aatttctttg
     3781 atatacaggc actacaaaac ttttgtttga cccctctaaa taactaagaa attcaatttc
     3841 tcctgctttc atattaactc cccctataag ttaaataaat tccattcata actccatgta
     3901 tctatatatt atatcaaaat tttgactcat aaatgtatat gaaagcctta ttatatacct
     3961 ttttatacct atttttattt tattaaagta aatacatgat tattctataa aatatacaaa
     4021 tacttaattt taaaaattat tatttaatct tcttcatgag taattcatag ttatttatca
     4081 attaaaaaga gatattgcat aattattcaa tacctctctt tgtatattta cattatccat
     4141 atctagttat ctatttcaac ccaatattag actacctact aataaattta ttctctacat
     4201 cattaacaat aatttttcac taaataacat cattagccta tctctgatgc attttcttta
     4261 attttattta tagttttaat caatttattt ctctcttcaa tatgattttc aaataaatca
     4321 attatacttc caacagttct aaatgggtct tccatcaaca ctttgttatc ctctataaat
     4381 ccattcttga ctacataatc gactataagt ttaacaaaat gtatttgctt agtattaaag
     4441 ctcttattat ttaaaaactc tgaaaaagct tcatttgcag cttctctatc caatcccact
     4501 agttttctca ctaattgact tactggagta tctccaaact ccttcttata atcatcacaa
     4561 ttacctaact gttttaagag tatttcctcc aacgtttcaa catcttgctt tgttatcttt
     4621 ttattatttc ttaatttaaa tattgctaat tcatctttat ggctatttaa ataatactct
     4681 accttctttt tataattctt aagattgttt gcattatata cagaatcatt tctatcttct
     4741 gctacaatca tatcttcaaa acttgtataa tatatctttt gactagactt ttcaagatac
     4801 ttaataagct ctcttaatga tatacgtact tcttctacct caaatatatc tgaatcttcc
     4861 caaaaatcct ctgtcattgc tttttcaata gtatattttt gttgctgaat ctgtggtata
     4921 gtcccaagtt ttgaaagttt ttctgctgta gccattacat gccttatacc tttacttgca
     4981 ttattatttt gtaagtatgc aagttgtata ttatacataa ctacatcaaa tcttttagcc
     5041 aattcatcat cactatatga agttatgagt ggtgatatat attctcttat ctcattttgt
     5101 gctactgctc caatattgct ccaaacattt tcatttttat acttatgtac ataacttaaa
     5161 ttcatcctaa ctatataact atcctcattt aaagctttta cagaagttat aagttcttct
     5221 aacaattctt ttctaagttc tgcatactca ccttctatat atttaatatc ttgaagttct
     5281 ttaaccatac ttacctttat attaaataat ttttcagtaa gtgtttctgt tttatttccc
     5341 tcaaatcctt taggattaaa tttaaaaaat tcaaaattat tacaaaaatc aaatattaag
     5401 aatctttcct tatccatatc aacacctaat aaatctggac ataatctagt tcctctacct
     5461 atcatttgcc aaaacttagt cttacttctc actttcttga aaaaaacaag atttagtatt
     5521 tcaggtatgt caattcctgt atcaagcata tctactgaaa ctgctatttg aggtaattta
     5581 ttcttatcag aaaaatcatc tattatagaa tctacataat ttatactata gtcaactgct
     5641 tttacaaagt tcccaccata ctttggatac agtttattaa atctttcaac tattgcccta
     5701 gcatgctttg tattctttgc aaaaattata gtcttaccaa tcttttcttc tccttctatt
     5761 ttcaacccct ctgtcattag cttatttaaa actaaatcta ttgtgtcaga attaaatagc
     5821 cactcattta ctgcattatt ccctatttcg tctccaatac tctcatcatc attaaatgta
     5881 ttttcatatt cttccttctc ttcatcagaa agctcatcat acttaattcc atcttccata
     5941 atcttagtct taaactcaat actagtataa tctactaaat atccatcctc gactgcctta
     6001 ttatactcat aagcatatgt aggtactcca ttttccatgt caaaaactga ataagtattt
     6061 ttatctatct catcctttgg tgtagcagta agacccatta agtaagaatc aaaataatca
     6121 aagattgact tatacttttt ataaatactt ctatgcgatt catctattat aattaaatca
     6181 aaatgaccag gtgtaaacaa tctctttcca tccttagact tcgtatcatc aattgcattc
     6241 atcattgttg gatatgtaga aaatatcatc ctagcatctt ctgggctatc tttgctatcc
     6301 aataaattac ataacgccaa gttaggtaat agcttagtaa aacttttttt agcttgcttt
     6361 accaatgctt ttctatctgc tagaaataat atatttttaa tccaattatg tcttgaaagt
     6421 acatcaacta tagatatagc aattcttgtt tttcctgtac cagtagcaca taccaaaaga
     6481 gttttgcgtt gcttattttc aagagcctca catacagccc taatagcttc tttttgataa
     6541 tatctattgg atatttcatc atttattgtt acagaagtta aagacttctt actctttctt
     6601 ctatctatca taagttgtaa ttctgatttt ttataaaatc cataaacttt cctatctgaa
     6661 tagtcatccc aaatatacat atcaaaacca ttagtcaaaa atattaccgg tctttgacta
     6721 tatctctttt ccaaacaatc tgcatataat ttagcttgtt gttgaccaat tttcgggtct
     6781 ttactcgttc ttttcgcctc aactactgct aatggcttac cattttcacc ataaagaaca
     6841 taatctacat atcctacacc actttcatta ggcatacctt gtacttctat ttcttcccat
     6901 atatcttttc caaattccca acctgcaagc tttaattcta tatttatata tctttttctt
     6961 gtctcaaatt cgcttacttc gtctatcttg aaatcatagt ttgcagtatt aacttttctc
     7021 ttttcagtta gtgactttct taattcctca ttttctttta ttacttcttc aagcttttta
     7081 tcttttgaac ttaatctctc ataaagattt ttaagttcgt ctggtctcgt ccttttctct
     7141 tccccatgaa gtaataaatc ttcattaaag tcttctgctg tatattcatc tgaataacaa
     7201 tagtctatcc aactaacaaa ttgatgcaaa ttgttaagtg aaagtattgc ttctcctctc
     7261 tctatgttcg cactagtatg tacagataaa ttacctaatt taactatata ttttaataaa
     7321 ggtaacatat catagtctat aagttctata aaactattat tatgaatcaa cgtactaaga
     7381 ttatcttgat aaggcaataa taaatcacta tcattagtat atagccattt tactgcaagc
     7441 tctaaagctc ttcttgttat agttgcacaa gtagatgggc taactagtat acttttttct
     7501 gcttctaagc aagcagtatt aaaactattg aaatttttct tcttttgtaa aaaatcaaaa
     7561 ttacccaagg cttttctccc ccttttgtcc cttagttaaa ataaatctct ctaaaaacta
     7621 tttcttacag ctgtttctta tactaacaaa tacttcttca ttttctgtaa acaattcgta
     7681 catatcttca aactgtggta tatcatatcc atgagaatca tcatttctca attttctaag
     7741 tgtttctata actatagaag aatttttagg taataaattg tgatactcac aagttaaaag
     7801 gtggtcataa aagtttttag attttatatt taaatcaatt gatttataat acaacattgc
     7861 ttttgttaat tctagaaatg ataccatata gcttttaaag ccttctttta tgtatctagc
     7921 ccataactca ggcatttctt ccttatcata taagattttg aagttttgca ggtcatacaa
     7981 gcaagaacca aaatccctat ttacttcttc tattgagttc caagataatt tgtttctaat
     8041 taccatacat gtccctccaa tctaagatat ctattacatc actgttttca aaatagaatt
     8101 tatttatata atccttaaaa tctttagatt cataaataac ttcaccttct aaaatagctt
     8161 ttttaagtgc tattgtataa ttatctgtaa caatttttag gtctacatca atgtctaata
     8221 tatttcttag agcttttgtc ttagttgcaa gttctgtaat tggtactttt ttgataggaa
     8281 tccaagctat gtctatatca ctttctgatg tatgtttatc tgttccataa ctaccaaata
     8341 ttatgattgc tagagtattt tcttttggaa atacatttat tattgagttt ttaatctcat
     8401 ttggtatatt tctcatttaa tcacctcttg tttattaggt aatattgctt aattgaattt
     8461 atatccataa tcttttaatc tatttataca tatttctctt tcttcatcag taaacaactc
     8521 tttatacttt tcttttaata ctaatacttc taatgataaa tctaatctct tacactccca
     8581 tagccttgta aatccttcag tagctccttc tttattgatt aattctttag cagctaataa
     8641 ggctcctttg ttatttatca gttgtaaaaa tcttgttggc ttatagttac attgtaaatc
     8701 agcttcttta tagatattta ccatgtcatg atataaatct agctctattt gattcataga
     8761 ttttattccc ccattttaat acttgtattt tagttgaata actctccttt aaaagctttt
     8821 tgcattaatg agttaaagtt gttttctagt tcttttaagc tatcttccat tcttgatttc
     8881 aatttgtcta tttgtctgac aaagtccaca aacttatttt gaagatatac aggtacttta
     8941 ggtatttcaa cttcatttaa tttgcctact gtaagacctg gttgtgctgc tccagttgat
     9001 aatcgattta aatctaactc tttaagtgta aaatatagcc agtaactatt tatatcaact
     9061 ttaggttgta ctacaactgc atgttctgta gcataaaact ttccatttac atattttaca
     9121 ttaccacata atgctccttg cctacctatg agattaaatg ttcccttatg tgaatacatt
     9181 tttacatatc cccttaaacc atttcctcca taacaaggat ataattcatg tgaatttttt
     9241 tcataaatat cttttgcttt tatatttttt ccagctttta agtttgatat ctctcctaat
     9301 aactctgtat cccaatcttt actattagta atcacatttc caaacatctc aataaatctt
     9361 gatttaacta attcatccaa tgcttctatt tgctcttttc ttttatttat taactcttga
     9421 gctttattta aaacttttac cattttattt tgtatacaaa ttgtgaaata tggtattttt
     9481 atatcattta acttgctttt atttaaagtt gcacccataa ctgctctatc agtattatta
     9541 aaatttaaag ttctcaaagc ataatataaa tattcagatg ttataagttc attatttttt
     9601 atttgaaagt tagcaattgc ttcatttgta aacatgtctt cttttaatat ggcaactcta
     9661 cctatagaca acttaaaact cattacaacg gtatttttag gtactaattt catattagct
     9721 ttttcaacag ctaaatctgt tatctcttct tttgattttg atatatattt ttctttcaaa
     9781 tcagaaattg ataaccatct attaccactt ccccaataat cagaagtatt tctagatggt
     9841 gttttgccaa tatttatata gcacaattca tttaatttta tatactccat accctatacc
     9901 tctaacattc tttctaattc ttctaatcca acagctattt ccttctctaa ctccctaact
     9961 ctacctataa tcacactagg agcatcataa actacttcct catacactac ctctttatac
    10021 ttattaatag ataaatcata cccattctct cttatctctt ccacatcaac aaaaaagctt
    10081 tgctctgttc tttttctatc tacttcacca tcaagattat caaatctctt aataatatcc
    10141 tctatatcac tatcctttat ctcatttctc ttatcatcca aagaataacc atcagctttc
    10201 atatcataga accatacctt atcagttcca cctgttcctg ttttagtaaa tatcattata
    10261 gctgtagata ctcctgcata aggcttaaac actccacttg gcattgatat tatagcttcc
    10321 aatttatgat tatcaactat ttcttttctt atcgatttat gtccacctgt tgaaccaaac
    10381 aatactccat ctggcactat acatgcgcat cttccaccaa tttttaaaac tcttaaaaaa
    10441 agtgccaaga ataataactc tgttttcttt gtcttagtca cttttaataa atctgctgac
    10501 actgcttcat aatctaaact tcccttaaaa ggaggatttg ctaacaccaa tgtaaactta
    10561 tctttatctg tattaacctc cgataaagaa tccttatact ctatattagg attgtcaacg
    10621 ccatgcaaca tcatattcat agcacctatt ctaagcatag ttctatccat atcaaatcca
    10681 tagaacatac cattattaaa atgctccttc agcccttgca ctaagaaaag acttgaatga
    10741 ttatccctta agtattgttg tgatgatact aaaaaacctg ctgaacctgc tgccgggtct
    10801 actataatat cttctggtgt tggtttaata agtcttacta tcatatctat tatgtgtctt
    10861 ggagtcctaa attgaccatt agtacctgct gtagccactt tggataataa atactcatac
    10921 aaatctccct ttgtatctct atccttactt aattctaaat tactaatacc atcaactatt
    10981 ttagataaca taagaggtgt aggaatttta aatattgcat ctcccatgta ctttgcataa
    11041 gctgagtctt tatttccatg taattttttt atgaaaggaa acacttcatt ttgtactatt
    11101 tcatacattt tttctgaatt tccttcatta gaaaacttac tccatcttaa atgttgatgt
    11161 tcatcatcaa atatcctttc aaaagaaact cccaaaagag ttgcttctgc ttcatttttt
    11221 gtttctactt catccaatcc tttaataaat aaaagatatg taaattgttc tataacttct
    11281 aatggattag ttaatccacc agtccaaaat gtctcccaaa tcttgtctac tttgctcctt
    11341 aattctcctg taatcatata tgactttccc tccagtgttc ataaaataat ttaaatatat
    11401 attttagtcg tacgccttaa taattagtgt cttatattaa attcaaaatt ttactacaca
    11461 gttattaaat tatttttcct atttacatgt tttagttaaa aaactaatgc aatactctta
    11521 ttttatgatt attatacatc ccatacaaaa ttattttatc atatatccag catagaagtc
    11581 atctacctgg ataactctta ccatattata actttttaat attaatttaa acttagaaag
    11641 aaatttttta tttaattata tcatcttcct tatttcaaat ttctcaatta caatctcaat
    11701 caaaaaataa aataagatgc tgtctcaaaa tagatataat cttctttttt attttcggaa
    11761 tgatgaaaat atatttaatt tcatacaaaa aagagtgcct cctgaactaa aaagttcatt
    11821 ttgagacacc ccttaattta atcttactta ttctctccct ttattattaa cacttcacta
    11881 tctctattgc ttttcctaca catccacaaa cttccatcct actcttaaca aaatctttaa
    11941 ctgactctaa acctttttta ccatatttct tagggtcaat tacctctggg tcagaattaa
    12001 gcacatcctt aaccccattg ctataagcaa tccttaattc agttgcataa ttaaccttgc
    12061 aaatccctct ttttatagat tccttaataa cttcatctgg tattccagac ccaccatgta
    12121 atactaatgg aactgataca acatctctaa tttcagataa tctatctaaa tcaagctttg
    12181 gctctccctt atataatcca tgtgccgtac ctattgctac tgctaaagat gatacccctg
    12241 ttctctctac aaactctttt gcttctaatg ggtctgtata tgctccccca tcaccaccat
    12301 ctaaatcgtc ttccttacct ccaactttac ctaattcagc ttcaacaggt atatctgaag
    12361 gcatacatgc atctacaaca gatttagata ttgaaatatt atcttcataa ctttcatgag
    12421 aaccatcaat cataatagaa gtatatccaa ctctaagtgc ctgcatagct agtccaaagc
    12481 ttgaaccatg gtctaagtgc attgctactg gaacacttgc cttctctgat gctaccttta
    12541 cattcgctaa ataataatct agtcctgcat actttattgt tgaaggtgtt gtttgcaata
    12601 ttactggtga tttaagttct tcagcagcct ctattactgc cataaccatc tccatatttt
    12661 caacattaaa tgcacctact gcataatgcc cttcttgtgc atctaataat atttgtttag
    12721 ttgtcactaa tgccataatt tctcctccta tatgtttata ctgatttgat ttcttcgtta
    12781 tttaattttg atactatttc tctacttgtt ttgctattta ataactcatt taaaaattct
    12841 tcattcatta attttgaaga tagttgagat aatgttttta agtgtaaatt atcaccatta
    12901 tctgtagctg ctatcataaa tataatatgt gctaagcttc catcttctga ttcaaagtca
    12961 aacccttctt ttgatacacc tactgctaca cgtggtacct ttactgcatc tgtttttgca
    13021 tgtggtatcg ctatccccat accaaaacct gtagatgata tttcttctct tttaaggatt
    13081 tcttctttaa acttcttttt atctaaaaga acaccagatt tatcaagtat tccaatcatt
    13141 tcatcaatta catctgtttt attttttccc ttaacatcta aaattactgt ttcttcgcta
    13201 tataaagaga tattattaga tacgttttcc gattctgcac tttcattttt atgttcatct
    13261 tttgttacat ttgtattttc tatagtattt ttctcattta ctactttagg tgtaaccatc
    13321 atataaagta cagcacctac taatgaacct acaaatatac accctaccca taaaagtggc
    13381 ttattgacta atcctagaac caaaaaccct ccatgtggag ctggtacttc tattttcatt
    13441 atataagtta aaattgatga tattgatgat gcaatcataa gtactggcaa cacttttaat
    13501 ggattttttg ctgcaaaagg tattgccccc tctgttatat gagtactacc tagtatataa
    13561 ttgattaatc ctgctgctct atcttcttct gtaaatctat ttttaaataa agtagatgct
    13621 attgctatta ccaatggtgg tgttatacaa gctgcagata cacctgccat aaataaataa
    13681 tttccctgtc ctagaagcat agttccagtt acatatgctg ctttatttac tggacctccc
    13741 atatcaaatg ctgacataca tcctattact attcccaaaa ttatagggct tgaattttgt
    13801 aaccccgata gaaagttcat catagcattg ttaagtgctg aacatggccc accaagtaac
    13861 aacattgcta cacccacaag acctacgctg acaataggaa ttataaaaat cgatttaaga
    13921 ccttctaatt gtcgtggtaa ttttcttaac aaatgtacaa atagtttaac taagtaacct
    13981 gctaaaaatc ctgatatgat tccacctaaa aaccccgaac ctgtatcaaa tgatatcatt
    14041 cctccaacaa gtcctgccaa cataccagct cttcccgata tagattgagc tataaatgct
    14101 gccaatacag gaaccatcaa gttcatagaa tatccaccaa cagattttaa tgtagctgct
    14161 atttgattat attgttctga atttgggtca aatgaataaa tcccccataa aaatgatata
    14221 gcagtaagta ctccacctgc tactactaaa ggtaacatgt gagatacacc attcattaag
    14281 tctttataca acttttgacc aaacgataaa tttccttctt ctgcaatttt actgcttgat
    14341 aagtttcctt ttctaatagc tacttttcca tcaatacatc tttgtataag ttcattagcc
    14401 ttatgtatac cttctttgac aggtacttct attactggct ttccattgaa tctgtccata
    14461 tcaacatttt tatcacaagc aactattatt gcatctgcca tttcgatatc ttttgctgtt
    14521 aacttatttt ctacacctat agctccattc gtttcaactt ttatgtcaca gcctaattcc
    14581 ctggctgaat tttttaatgc ttcttctgcc ataaaagtat gagctattcc agttggacaa
    14641 cctgttaccc ctagaatcat tttctttccc atttttatac cacctttctg actcttatat
    14701 tttttatata atcttccaca ttctccaaat tacctatacc attactttca gccacacttg
    14761 ctcctgttgc tgctgacctt tttaatgctt cttcaatatg ttctggattt tccaaccata
    14821 tacttaaaaa agcagctaat gcagaatcac cagcacatgc agagctgact actgtaactg
    14881 gttgagcact agcataataa attgattttc cattataaaa ataagaacct ttttctccta
    14941 aagttagcaa tatattttga gcacctttta aatgcagtaa tttaagtaca tccttaatat
    15001 cttcttcatc tcttacaata attccaaata tatctttgat ttcttcatca tttggtttta
    15061 tcaaatatgg attatactgt agtaactcct ttaattttgg tgaacttata tcgagtattg
    15121 tctttatact tttattttta caaattttaa atatatcctc atagtaatta tcatcaatac
    15181 caggaggtag acttccactt attgataagt aacttatatc tgctaatgac tgtatcttat
    15241 ctagcatatc tagtttttgt ttttcattta catatgaccc agagtttaca aatttaaatt
    15301 catcacttcc atcatttaaa aatatattta tacgagttgt atcttctacc caagtaggca
    15361 atacgtcaaa tcctctcttt tcgctttctt caactatata ttttcctgaa aatcctccaa
    15421 aaaatcctaa tatcttagat tctataccat aatgacctag tacaaaagtt acatttaatc
    15481 cctttccatt aggagaatat acagcatttg atgtacgatt tactaatttt ctctttatcc
    15541 cattagtaga tatattcata tctattgcag gattcgtagt taatgtatat atcattgtct
    15601 tcccccttgc tcgtgctgtg tatgttttca ttataataaa aaagtctatg aaaatctttt
    15661 cacaaataga aaatcgattt tcatagactt ttcaatttaa ttttcagaat tattatatct
    15721 gaccatataa tctataatta tcctagatat aatatacaag ggcaatctgg aagatacttc
    15781 atattcagaa attgatatat gttcatgttt gtatcctaca atatataaat ccgaatactt
    15841 tataagtttt gcactttcac tacaacagca agttataacc ttagctccaa ctaaattggc
    15901 attctgtgct gattctacaa gctctatggt atttccattc aatgaaaata taattataag
    15961 ttcgtctttt tttattgatt tagattttat tttcattatg ttggggtcat ttataaaaac
    16021 aacattaaaa tcaagtaatt gcaatttaag actaaactct tttgctacat attcagtaag
    16081 ccctcttgcc aaaatatata tcttactaga tttatttata gtttttatta tattgattag
    16141 tgtagttaaa gatatttgtt ctataactct ctgtgcttct ataaaggatt tatttattat
    16201 atcccccata ttttgtatat catcattttc atcttttact gttaatctat acctaagttc
    16261 attgaatcca tttaaaccac attttctaat tgctcttgaa acggttgctg gagatgaata
    16321 tgtttcatga gcaatctcta ctatagataa ttctggtaat attgattcat tttcatttat
    16381 aaatttaatt atttcaagtt ccgtcttcgt tagattttca gaattacttt tgtttagctc
    16441 aattatcata tgttatctcc ccttatataa gttttatttg tctaaattta tcgagtaata
    16501 taatatatta cctcttaact acttattttt atatacaatt aagatttagt caatgtctat
    16561 atatgtaaat ttataatata ataatttttt gcttaaagtt tattatgtta tcttatagca
    16621 ttttaaaata aaaaagaatt gtacctctac caaaatttgg
//
LOCUS       DAGSWI010000091        16536 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.091, whole genome shotgun sequence.
ACCESSION   DAGSWI010000091 DAGSWI010000000
VERSION     DAGSWI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.091"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(174..1364)
                     /locus_tag="MJF80_002638"
     CDS             complement(174..1364)
                     /locus_tag="MJF80_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBZ0281290.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(1380..3335)
                     /locus_tag="MJF80_002639"
     CDS             complement(1380..3335)
                     /locus_tag="MJF80_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBZ0281291.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKASTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSEPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(3352..5418)
                     /locus_tag="MJF80_002640"
     CDS             complement(3352..5418)
                     /locus_tag="MJF80_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBZ0281292.1"
                     /translation="MKEVVGSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPNG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKDAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVVDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMNEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            complement(5734..6384)
                     /locus_tag="MJF80_002641"
     CDS             complement(5734..6384)
                     /locus_tag="MJF80_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0281293.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            complement(7328..7663)
                     /locus_tag="MJF80_002642"
                     /pseudo
     CDS             complement(7328..7663)
                     /locus_tag="MJF80_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(7761..8441)
                     /locus_tag="MJF80_002643"
     CDS             complement(7761..8441)
                     /locus_tag="MJF80_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281294.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTLVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(8438..9364)
                     /locus_tag="MJF80_002644"
     CDS             complement(8438..9364)
                     /locus_tag="MJF80_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281295.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVNPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(9437..10354)
                     /locus_tag="MJF80_002645"
     CDS             complement(9437..10354)
                     /locus_tag="MJF80_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBZ0281296.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLVEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(10354..11043)
                     /locus_tag="MJF80_002646"
     CDS             complement(10354..11043)
                     /locus_tag="MJF80_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281297.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIRYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(11451..11756)
                     /locus_tag="MJF80_002647"
     CDS             complement(11451..11756)
                     /locus_tag="MJF80_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281298.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            complement(12699..12917)
                     /locus_tag="MJF80_002648"
     CDS             complement(12699..12917)
                     /locus_tag="MJF80_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281299.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(12918..13325)
                     /locus_tag="MJF80_002649"
     CDS             complement(12918..13325)
                     /locus_tag="MJF80_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281300.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(13411..13899)
                     /locus_tag="MJF80_002650"
     CDS             complement(13411..13899)
                     /locus_tag="MJF80_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBZ0281301.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     IAVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(14104..15225)
                     /locus_tag="MJF80_002651"
     CDS             complement(14104..15225)
                     /locus_tag="MJF80_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBZ0281302.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(15598..15768)
                     /locus_tag="MJF80_002652"
     CDS             complement(15598..15768)
                     /locus_tag="MJF80_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281303.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(15940..16383)
                     /locus_tag="MJF80_002653"
     CDS             complement(15940..16383)
                     /locus_tag="MJF80_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0281304.1"
                     /translation="MIEIKTIEINKKSQIDLLMLADPCEEMIDKYLNKGTMHALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         5505 a         2852 c         1735 g         6444 t
ORIGIN      
        1 tttcttgata aaatcaagat tataatgcta aaaagaaaat attcttataa taaattaact
       61 aatataaaat tcgtttataa attaatactg actttctagc tttatttata aattaatact
      121 actttttagc ttctcctata aattaacacc acttttttaa ctatgaaacc tatctacaac
      181 attggtacca taagaccagc taccacaata gaaatcatac aagccatacc accaactacc
      241 agcttaggca taatcttaga catcaaatat gctctttctt cttccgtttc tccaacagca
      301 ttacacactt catttgaaat taaatatgta gttgggaatc ccaacatctg acaaaaccct
      361 actccaaaag caagaggttt agaaccgata attttccaac ctggtaacac cttcatcata
      421 aggaatatac caatgataga agctgcaaac atacctacaa ttggtacaaa aagactaatt
      481 aaatctgtca aagaaacttt tgctaaagaa ggaataacag ctgcaaacac aaccatgttg
      541 ataaatccct tgatttttcc tttttccaaa atgtcttttg gtacaatacc aatacgtgtt
      601 acaataacac caacaacaag acatacaata gaataattta catgaataat ctctgaaagt
      661 tcatacccta gataagagaa taatgctata aaaaatatac aaacatttgt agaataatat
      721 ttatcgtatt tttcacagaa agcttgtttt acttttgtgc ctgtattctc tgcttctttc
      781 ttatcagtat caacatttgc taaatcaatg ccttttgatt ttgcttcatg atattctact
      841 aaaagacggt ttgcctcttg taacgctgct cttgaagcta ttggtgtacc aataaacttc
      901 tgaattgcaa atacaactgt tgctgtagct gctgcaagtg tcagtccttt ttctagtgct
      961 gcttgtgtca taatagtagt agcaggtaaa gctccattta taactggtat gcttgtcaat
     1021 actgttgatt ttccaaacaa aggaatacat aatgagacac agactataac tgctacaatt
     1081 ccaagccatg ctgtcaatac tgttctccat tcgtctataa attgtttaac attcaacatt
     1141 gttcccatac taaacataat cattggaaca ctccaactag ctgctgcggt catacctgct
     1201 ttttcaataa tatcagctgg tattgctttt gtgacaaata gaatcaaaaa taaaagtagt
     1261 gtaacaaata cagctgatac ttttgctttt gttttgcttg caattaaatc cccaatagca
     1321 aataaaacta atacgacact aaaccataat aacatatcac tcatagaata acccccttat
     1381 tataaatatc tttcaaacca atctacaata gcatttgctc ttgcaattct attagatggt
     1441 cttccatttc tagacaatcc atgtgtatct ccgtggaaca aatacatttc tgtatctaca
     1501 ccaagctgtt ttaatgctgt aaacatttgg aaagtatccc ccataaaaca tctataatcc
     1561 ctatcactat gtaagaacaa tgttggtgtc ttaacatttt tcacatattt aataggtgaa
     1621 tgattccaaa gaacctcctc atgattccaa ggcgtcttat ctccacaagt ctgcttcata
     1681 attctatccc caaagtcaat aattaaagaa gctgtaatca agttactaat acctctttga
     1741 gaaactgctg ctttgaaacg gtctgtatga ccaattatcc agtttgtcat ataaccaccg
     1801 taacttcccc ctgcaacccc taagcggtct gcatcaatcc caggatattt ttcaatacaa
     1861 gtatctacaa attccataag atgctcatag tcaacatacc cccatctttc taccatttct
     1921 aagaattttt caccataacc gtcaccacca tgagggttac aataaatcac atacattccc
     1981 tttgatgtta cacactgctt caagtgtgta aacatatagc catatgccca tttaggtcca
     2041 ccatgaatga acaatacagc aggatatttt tcgccttctt tagcatctat aggcggtata
     2101 acaaaaccat caacttcttc tttatttttt gctacaaacg tcaatttttc aggtaatgaa
     2161 accttatgag tatctaaata tggttcagaa aaagcagtca tttttttcat tgtacctgta
     2221 gtcatatcaa tgctatatat ttctgctaac tcataatctt tcaacattac tgtgtacatg
     2281 gtgttgtcgt aaatatcaaa agatgtaaaa gcatctactt ttgtgatgat acgtatatta
     2341 cctgtctttg tatcaaactc attaaattct gtcatagttc tgcaaattct tgcaccaata
     2401 aatttgtctc ctataacttt attactacgt ccaccaccat atactgcatc tacatccagt
     2461 aatgtagcat acataccatc tggactagca atcatattta tttcactagt actcaaatca
     2521 agtataaaaa tatcatgatt ctgtgtaata gaacggtcta atgttgaagc ttgcaacatt
     2581 actttatctt tgccaatata ttttgccaaa cttatttggt actttccttg tggtacaagt
     2641 tctttggttt caccactgtc tatatgatac tcccaaagtc cagaaaaagg agttgcacaa
     2701 tcttcataaa tctgaccagt atataaaatt ctgtttttat cagaagaaac gtctacacct
     2761 gctacttcac aaaattttgg tgtaatggct tttgttatat tttcaccttc ttgatataat
     2821 tctaatgtta cacgtcttct ttgagaaaaa ttttctccat ctcttataaa aggtttatct
     2881 gtaaatgtcc aataatctac accctctact gccctatctg gttctgattt tttagaatcc
     2941 gttggatttt ttgttgtcag taaccacaat ttatttccaa gtgatacagg tacgctacaa
     3001 gcttttggaa tagaaaatgc tttttttgct tctcctcctt ctatagaaat agtgtaaaaa
     3061 tctgttgtac ttgcttttgg cttatttctt ttagatgtaa aaagtagttc tgtattagaa
     3121 atccaactaa atgatgttac atcttttgtt tgagtcatat gtatttgttt ttctgttttg
     3181 atgtccatca catataagtc atgttcatag cagtcttttt cctcgttgat atttgatata
     3241 atataagcaa tatgctttcc atcaggtgaa caagaaatat catgagggaa tatatattca
     3301 aaaatatctg aactttcaat ctttattttt tccattttta tattctcctt tctactgtcc
     3361 tatgttatct ttttttatat ataccgaaaa ccaatctagc atttcaacta atctttgtag
     3421 cttttgtttt ggtctaccag aacgagacag ttcatggttt tctccttcaa atagacaaag
     3481 ttttgtagga cacccatgaa ttttcaatgc tgaaaacatc tggaatgctt cttgcatata
     3541 gcatctatag tctttttcac catgtaaaaa taaagtcggt gtaacacatt tatcagcata
     3601 agcaagaggt gactgtttcc attgaagctc gtggttttcc atatgagaag cacctgtttg
     3661 tcctttgcag aaataataac caatatctgt tgtaccctcc atgccactcc agtttgcaat
     3721 agaacgctga gcacaagcaa cagcaaaacg gtttgtatga ccaattatcc aattcgtcat
     3781 aaatccacca taacttccac cttcaacagc caattttgtt ttatcaatgt ctgtatattt
     3841 ttcaagtaca gtatctgtaa attccataaa atctatataa tcaatatctc caaactgttt
     3901 cctaatatct gcaaaagcat tgccacgacc ttcagacccc cttgggttac agaagaatac
     3961 aaagtaacct gcacttgtaa atacctgtat ctcattcata aaatatggtc catatgtcaa
     4021 tcttggtcct ccatgaatcg ccaatacagc tggatatttt ttccccacct cataatctac
     4081 tggtttatat acgtatcctt gtatttccca tttagaactt ccttttgact ctatgtattc
     4141 tggcacagaa attttatatt cctctaaaat atggtcattt aaatgtgttt tcttacacaa
     4201 tgttccattc tctactgtat aaacttctgg taatccattt cctaaacaac ccacacatac
     4261 aatattgcca tcttttacat ctataaaatc aactgaacct gtctgtgtta ctttttcctg
     4321 ttttcctgtt ggaatatgta ttctttcaat aatgcagtcc atatctactg tagaagtgta
     4381 ataaatatat tctccatcta cctttgttgt ctgaccagca cccattttaa catcagatgt
     4441 tactgtattt ccaatacaat gatgttgatg ccctgaaaga agtttcattt ctttcgtatc
     4501 aatatcaata gtataaaaat ctccattttc tccatttcca taagataaag catcagttag
     4561 gtttagtatc acttgatttt gatataattc aaaacctttt ataatatact tatctttttc
     4621 taatatacag acatttgtgt ttgtgtttgt atcaaataca tagaccccat ttttcaaacc
     4681 ttttacatct ttaaactctt gccctatata taagattttt ccatcactta ttttcatacc
     4741 tacaacatta aacataggtg ctgtaattgc cttcaactca tttatattac tgtcgtatac
     4801 atataggcgt tttctttttc tgctaatata tccctcccca ttgacagcat aaggaatttc
     4861 tttgaaatgt tcataatgtc ttttgttata agcatttatt tttttctgtt gttcttcttc
     4921 tggcaatccc tctacaatag gtcggtcatt gtcataagaa tcaacaactg catacaaacc
     4981 ttttcctata ggattcaatt taccaccttt gatagggagt ctaaaaagtt cttctgcttc
     5041 tccaccattt aaagataaag catagtaact catgcattgc ttaccgtttt tgacagcatc
     5101 ttttactttt tcatttcgca aagcagggaa caagatagta ttttcatctt cccaggcata
     5161 tgctccaaca gagtttatag acgttatttg ttttaattcg ccagttttat tgtttaatat
     5221 ataaacttgg cttgtgtaac cattctcttt gatatcagct ttttttacaa taaatgctgt
     5281 atatcctcca tttggtgata tttggcaatc acttataaat tgatattcca aaaatgtttc
     5341 actcgtaaat agctttttca tagtattttc tatcctttct ttagtaattt ttttcaaact
     5401 ccctaccacc tctttcattt tttattttaa atttaaaata aaaaatttta aatttaaaat
     5461 aaaaaatttt ataaaggtta tttttttagg aagccttctg gcttccttgt aatatttgta
     5521 atttaaagta tatttaatcc atgcttaatt catacttaat ctatacttag tccatactta
     5581 atctatattt aattcatact tagacttatt tacactatat ttagattata tttattagat
     5641 tatatttaga ttatgtttaa actataatta gattatactt agactacatt tagcaactta
     5701 atagataagt atatatagta acttcaaaac ttattattca tcttcacaga atcttttaat
     5761 agttccttga atatgatttc ttagtttatc tactgctttt tttgtatctc tgtcttcaat
     5821 gaattttaat atttcaaaat ggtcttgtac tgttccttca taattacgat tttttaatgg
     5881 tcgaattaaa tagttcacta ctataaatct tccttgtata ccaacatata catcatttaa
     5941 aatctcatta tttgcccctt ctacaatggc tttatgtaat tccaaatctg ctagtgcaag
     6001 ttctacagat ttttcttgac gatagtactc ctcgcattta tcagagtatc tttttattac
     6061 atctagcttt tgttgaaaaa cctcttcctc ttcttgcatc attttttcta aagcatatgt
     6121 ttcaatcagt gttctcgttt cacaaacttg ttttaccata tcatattcat actctggtac
     6181 gtacacttta ccatttgtca taatagtaag cactccatct ttatttagca tttttactgc
     6241 ttgtacaact ggtgttctgc ttacttcaaa tttttcacaa aggtcattta gtaatatttt
     6301 ttgacctaca gagtattcac cacgaataag actttcaata atcttatgat ataaaatttc
     6361 atctaaattt gactttactt tcactactct caccttattt ctattctttt gccatattat
     6421 attgaattac tggactttaa ttacttttag taatattttt aaagtagaaa ttcttatttg
     6481 atagtacttt tgtactacaa tttaaattat tgtactgtaa atcatgctct tatactatag
     6541 attaattcat tgtattgcaa atcaagttat tgtactatag attaaatcgt tgtacttcaa
     6601 atcaagttat tgtactatag attaaatcgt tgtactttaa attaaactat tatactatag
     6661 attaattcat tgtacttcaa attaaactat tataccatag attaattcat tgtattgcaa
     6721 atcaagttat tgtactatag attaaatcgt tgtacttcaa atcatgctag tgtactataa
     6781 attaaatcgt tgtactttaa attaaactat tatactatag attaattcat tgttctgcaa
     6841 atcatgctag tgtactacaa attaaactat aaatgtttac tatctcttaa taatttgaat
     6901 aatatgttac aaaaagtata gcaccttttt atttttttcg caataaataa aaaatttaaa
     6961 attttttatt ttttattttc ttatattatc aatttgaaat acgaaagatt aagactaata
     7021 gaattgattt agttaattga aaaaaatagg taccttttat gaaaattcta atgataagta
     7081 tttttctgat gtaatggtaa attataaatg ataaaattaa aaatcacttt tagttttatg
     7141 aactgtcccc atattaagaa acaactaaga tacataaaaa tagttataaa gagatagtta
     7201 aactatatcc actattttat atagttaaaa tagtgagtat agtccaagct aagaataact
     7261 aagaaattag cgttaaatag catagataaa aatatattta tataaaaact ttttttgctg
     7321 caaacaatta tttccataac attttatata cttctctaaa atcttctaaa cttgataaat
     7381 attcaattaa agggacataa ttacctaaac tacaagaatt taaggagttt ctatacaatt
     7441 cctcactatc ccaagataca atttttatag gaagattatt ttctaacatc tgttttagca
     7501 taacaaatct acctatgcga ccattaccat cttgaaatgg atgtatcaat tcaaatctat
     7561 aatgaaattc agctataact ttaatagata cttcactctg actatagtac cattcaatta
     7621 attcatcaag ctttggctca acttcaaatg gttgtgcaat attattaaaa ctctaataat
     7681 attaataata gctgatataa tagcaaatca ttattatccc aagaagatac aattaataac
     7741 taccttccta gccacagtat ctagtcaatt tcagtaactt tatgatttag tatataatat
     7801 ataccaatca cagttatacc aactataata aaaacagaaa tcaaactgtc ttgagttgta
     7861 tttgtaccag ctaaaataaa ttgtcttaaa ttgataaaaa taattgaact gacaagggta
     7921 acaggtattg attttcttat cattccaaca actaatgtcc atatacctaa tgagcaaaat
     7981 acaataatac ttttaattgc tcctggtatc caataagata aaattgaaat atgaaaatct
     8041 ccaataacta aattaaaata cttatccaac acaacaacga atgtaaaaca acagatatat
     8101 ccaactatca ttgatgatat cataagagtc gtaatcaaaa gtaactttga taaaataagt
     8161 tttttacggt caagtggata agagaataaa atcaagattg ttttatttgt atattcattt
     8221 attatatatg ccacattcaa cacagcaaat aatataatat agtaaaaaca gaaaaataaa
     8281 ctgattgtcc taaatgtaac atcataatca tatattgaag tagatttatc attcaatcca
     8341 gtaaagacaa aaaacataca taagaaaatc cccagcattg aaaataaaaa atatcttttt
     8401 agcccaactt tttttagctc tagtttcatt aaatgtatca tagtgtttct ttacctcctg
     8461 tacctgtagt ttggaagaag taatcttcta aagaactttg tttacgtcca atgctattta
     8521 taccaatttg attttgaata aatattttgg atatttcttt tacatctttt cgtaaatcat
     8581 atatttcaat acaactttcc gaagtaattt taaagtcttt tattccaaat tctttttcta
     8641 acaagtatcc agcaagctcc acattatcta catctacttc aacataatct gtttgatatt
     8701 tgtgtatatc tgacatagaa atttctttaa taagtttacc atcttgaata attccaatag
     8761 tgtccgcaat ttgttctacc tctgaaagaa tatgactaga aataaatata gttattccat
     8821 aatctgtatt taacttacgg aataattctc gcatttctct tatcccctct gggtcgagtg
     8881 catttatagg ttcatcaaga atcaataatt ctggtttagt aagaattgct ctagctattg
     8941 caagacgttg tttcatccct aaagaatatt ttgaaacttt cttgtttgat tgctttgaca
     9001 atccgacaca ttccaatacc tctgatatat gttctttgtt ataataaccc atatattcac
     9061 agtgtaaatc aagattctgt ttagcagtca aattctcata taaatacgga ttttcaataa
     9121 tagtaccaat cttttttaat atttcaaaat cagtttctgc cactttctta ccgaacataa
     9181 ctatttctcc agaatttgga ttaacaagat ttagcaacat tttcattact gtacttttac
     9241 ctgccccgtt tggtccaaca aatccataaa tttctccctt tttcacatga atattaacag
     9301 cattaacaac agctttatct ttatatattt tctttaattg atgggtctct atgatatagt
     9361 tcatcatact ccctcctttc taaaaacagt atacaaaata aaattatctt ttctctcttt
     9421 taaatctaac ttttttctaa cttttcaatt ttaccgtaaa aactgttttt acattaggtc
     9481 tactatttac ataaattgaa cctcccatag agtttacaag cccttgagaa atagccaaac
     9541 ctaaaccatt gccttttttt ctgtcagcag tataagctct tgtgaaaata tgctcttttt
     9601 cagtttcaga aatacctgaa cctttatcct caatatctat aaaaacatgt ttttcctttt
     9661 catatagtga aatccctaaa aatctaccat ctgaaccata ttttatagca ttatcaatta
     9721 aatttttgag tatacgttgt aatgcttctg tgtcaacatt tgcatatata ggtctatctt
     9781 caatattaac ttcaacatta aagttttctt tatctagcaa atcataaaac tctaaaataa
     9841 tctcatgaca taattgtgtt agattggatt tttgaagatt taatatcata tctccagatt
     9901 ggatttttgc catattaaac aggttattta ttgaattaac aagctcattt gaatttgttt
     9961 gcattttttc cacagttgct ctcattgaag aagataattc ttctttttga ctttgaagat
    10021 atagcatttc aatataaccc tttaatactg tgatgggagt tcgtaaatcg tgtgatatgt
    10081 ttgtaaatat ttgcaatatc gctttttcac ttctattata attaatttgc ctgctgtaaa
    10141 acttttcaac aagtttattt atatctactg ctgtttcttt tacaatcgaa atatcagatg
    10201 gtactaaaat ataattaatt tcatcatcat ttgttatgtc acgaagtctt gaattgatat
    10261 acttataatc actttccatt ttctttgatt tatttcgttc ttttaagtag agagcaaata
    10321 gtacaatcga aattacaact aaaaataact ccattattct tcctccattt tgtagccaat
    10381 tccccaaaga gttttaatat aattattttt ttctatacca tcagacaatt tatttcttaa
    10441 cctgttaata tgagtattta gcacattttc atttccataa taaggttctt tccaaatagc
    10501 gttgtataat tgttcctttg aaaaagcacg tccttgatta gtcgctagta atttaacaat
    10561 ttcaaattct gtatgtgtta agtttaagtc aattccattc tttgaaacta catattttga
    10621 tatgtttata atcaaatcct tataccgaat aaccatatct tgagagtttc ttgaactgct
    10681 atacactgtt gcacgtctaa tattagcttt aattcttgca agcaattcta cgattgaaaa
    10741 aggctttgta atataatcat ctgcacctag attaagccct agcattttat ctgtgtcact
    10801 atctttcgca gtagtaataa taacaggaac aacactcaca cttcgtatat attttaatac
    10861 ttcaaaacca ctagactttg gtaacatcaa gtccagtaaa ataatatcaa atgaataatt
    10921 tttaaatatt tcaatagctg tatttccatc aatggcagtt ttaacagagt aattctcact
    10981 aatcagaaaa tcgtatacca tttctcgaat attcttatca tcttcaacca gtaagatgtc
    11041 cattattatt caactccttt tcgtcagtat acttcaaatg atatatttat taaaaagggt
    11101 tgtcaataaa taactgctta tgatattgta tctgcaattt ggtgatactc ttctaagtta
    11161 aagttctttt caataagctt tattgaaaaa agaattaaaa tatagtatac tctcttatct
    11221 ttatctgaaa tatacataat tttcttttat taacatctac aattaactat tttatattat
    11281 aaaacacctt ttaacttttg ctaaaaaata ttttatacat aaaaaagaaa ccacattcta
    11341 gttttataca gaacatagtt tctttaaaaa taatcaaatt tggattttaa ataattaaaa
    11401 acaaactttt atttgttttt aattttccaa ttttatagta gataatttag ttaattagtt
    11461 cttaaatgtt tatcttcttt agtaaatttt tttcttatta agaaatatgc aactattaca
    11521 acaagttcta ttgctaaaaa tacaccataa gtagctatag aatctatgct atctatgcca
    11581 tttctaaaga aaattaaagc aattcctgct gctataatac ctacagcatt tttccctata
    11641 gaattttgac caaaaccata aaaagaaggg aaatttcctt ttatttcaat gaatgtcaag
    11701 attaatccaa tagctataaa tagtactcct acaacttcca taatcatgct aggcattttg
    11761 acccctcctt attaataatt aactactact ataaaaatat ttattatttt tataattata
    11821 ttatatatgc gattacatgt ttttggaagt atatttggaa gtaatttcat atttatttaa
    11881 cttaattttt ttgtgataaa ataattctaa ttttcatata gatattatta taaattttct
    11941 agtttatgga gtcattcatt ctataattca caaatatgta ttttttaatc tattaaagta
    12001 ttcattttct ggcttataag catggacttc ttatctataa gcttggtttt tcatctaata
    12061 agttatttat tctttaatct atgagcttga tttttcatct ataagtcatt tattttttaa
    12121 tctataagtt tagttcttca tctataaatc atttattttt taatctataa gtttggtttt
    12181 tcatctataa ataagtcatt tattttttaa tctataagtt tagttcttca tctataaatc
    12241 attcattttt taatctataa gcttggtttt tacctataat taattcattc tctaatctat
    12301 aagcttggtt tttatttata agtcatttat tctctaatct ataagcttgg tttttattta
    12361 taagtcattt attctctaat ctataagctt acttcttaat ctataagtca tttattctct
    12421 aatctataag cttacttctt aatctataaa tcattcattt tttaatctat tagcttgttt
    12481 tttacctata attaattcat tctctaatct ataagctttc tttttcatct ataaatcatt
    12541 cattctttaa tctatgagct tggtttttca tctataagtc atttattctc taatctataa
    12601 gcttggtttt ttacctataa ttaattcatt ctctaataca taaacttgaa ttttttatct
    12661 attaattcat tctctaatgc ataagtaata catgaaattt aaatattatt ttcttcaata
    12721 aattcaaaca actcttctac agttgtatca aataccttag caatatccat tgccagtttt
    12781 aaagaaggat tgtagcgacc attttctaaa tgtacaatag tttcacgtct tactcctact
    12841 gactttgcaa gttcaccttg tgtcataagg ttcttttctc tataggattt aatttttgtc
    12901 tttagtaata ctgacaccta attgtatatc ccctttctgt caaaatatat aaatagaatt
    12961 aacttcaaca aggattgaat aaacaaaatg actaataaag acattcctat atttaaattt
    13021 gacacattta tgtccaaagt tacaagaaat atagatacta tagctacaga aaaaaacaat
    13081 acttttattg caatatcatt aactcttgat agtatacatt ttgatgcctc atcaattaaa
    13141 tcatcatact ttttattata gacccactga gctcccatag ctaataccca aataattata
    13201 ggaagatatt caatactact tcctttaaaa aaatacttat ttactaatgt agctccaata
    13261 caataagaaa ttcccaatat ttctgaaata agtgttgatt taataccagt ttgttgttta
    13321 gacatataaa atccccctta aaaagttatg tatttataac acctcttgtt ataaatatat
    13381 aacttttttg aataaatttc aataaaaata ttattttgca acaaattctt ttattaattt
    13441 tacattagat agaggcactt tagatgtcac tccttgcaat gtcaaatctt ctatattaac
    13501 ttcatttaat tttgaacaca gttctaagtc aaatattctt tcccaactta ccttcttttc
    13561 aatttgttca tcttcataaa gctctacagc aatatccatt aaaggtaaat gccataaatc
    13621 aaattctgat aataacacat tatcttcact tatttctatt tcaagtaaaa cacctttttt
    13681 tcctttttct aaataaccac tacatcttaa atcaggtctc ttcgtccaaa cccatatcat
    13741 actatcatca aattcatata ttctatttgt catttgacta tacatccatt tgtatggaat
    13801 atcaaagtct tctaatataa aagaataatc atttgataga tatttattgt ttttattgtt
    13861 attccaaaat tctaaagtct gtatagtata aaatttcata tataaatcaa cccctttgtt
    13921 tttctataag aaggagatgg ttcaaacttt tattttgaat tatctccttt atttatacac
    13981 ctctttgtac ctattattta tatatctctt tatatctacc atatttaaat agcaaagttt
    14041 tatattaatt taaatttaaa agaggaagct ttgtatttat ctagtgccat ctcaatcata
    14101 gtattagttt tgattataga ctttttcaac ctctgattta ttcaaatcat ctataatatc
    14161 gcatatttca ctcaaattgt atcctttatt ttttaatttt atgatgatat taaatttctc
    14221 ttctcttttc ccttttgcaa tacctttttc gatacctttt tcgatacctt tttcaatacc
    14281 ttcttgaaaa atttcttctt taaaatttct acttttattc ataatatacg cacctctctt
    14341 taaatttaat aaatagtcct cattactacc taattctttt aatctatcaa gtgctctttt
    14401 aataacataa tccgttttag atagtattct aatatctata tgagaaggat tatcaataag
    14461 tttaccccat attagaactc tatttgctat acaactggat ttacgaattt ttggaagttc
    14521 tataaaatat aatgcagttt tactagaaaa aatatctgga tgcccgtcca ttgttaatct
    14581 caggcaattt acataatctt ctatgtgatt atattttaca tgatttagta tatgtattcc
    14641 aacagttttt ccaatttctt tatattcttt accttcttct aactttctac catgtacacg
    14701 actaagataa tattcagctc tttccaaata atcctcttca agagctcttt gcatctcaat
    14761 atttatatgg ttacgttctt catcttcagc caaaatatca agtctaggtc ttttctcacc
    14821 aatgtaatta attgttgggt cggtttcaac cttaattatt ctttttattt gagtgaactg
    14881 tttgatattc ctattagcat atggagcatc tttgaggata tcatttataa aatttgcaag
    14941 taaatcgtaa tcattattta aagtaaacaa tgatttaaat acctcatcat actcaggctt
    15001 agcataccca tcttcagtta caacttctcc aacattttca ctagtcaata tttcttttgg
    15061 aattaaatcc ttgattttat ctaatttttc attagaaaaa tcacctctac taagtatgtc
    15121 atttaatttt ttggtccatt ctctgtcctt ttcttcaatt atagttcgtt tatactcttt
    15181 tgatgtttta ataatcttag atttttcttc taatttttct ttcatatcaa cacctctttt
    15241 ttattattac ccatttcttc ataccaaagt ctgaacttct aagtaaaatt tgcattattg
    15301 aaataagaac cttataaact tatatatcat tactaaagtt aagactttaa agagaagcac
    15361 ttagattaca atagatttta gatagctttc tttttaaaac tattgttgat aaatgaatga
    15421 atataagggt atgtattaaa atagagatac tcatcttttt cattgtttaa ataatgaaaa
    15481 tatattttat ttaatacaaa aaagagtgcc ttataaacta aaagttcact tcgagacact
    15541 cccttacatt ttgtttctta aactttacaa tattacatac ttatttatta atttaatcta
    15601 tgcatactta acccaataat aaagtacata tatccaactc attaagccat gaaaaatagc
    15661 ccatggaata gatttccatg cagtataaga aataatcatt gctaaacaag aaccaaaaga
    15721 aatacccttt ttaacagttg attttatagt attattattt gaatccataa ttaatcctcc
    15781 aaaattcaaa ataaacaagt tattatatat attatttgaa tataatacaa tgtaacagct
    15841 tctaaataaa ttatactctt attatacata atttataaat tataattccg ataattatat
    15901 gaattaatta attatattaa cctaaaaata ttatcctatc taaaagttat atgatagata
    15961 caacatgtca acacattgta attctccttc aaagattggt tctggataat tatctacaaa
    16021 aaagttttta attttatgtg aataaacaaa tccaaacttc tcataaaaag gaactccgct
    16081 ttcagatgta ccaacaacca ttgatgaata ttttttacta taaacatcta aaagatgata
    16141 aatcatcttt gaggcataac ccatattttg aaaatgctca tatgtagcaa tgttttttaa
    16201 ttcacatatt tctttggaaa tttcatttac aactgctata caagtaagtt ctttattatc
    16261 atacaatgcg tgcatagttc ctttattgag atatttatca atcatttctt cacatgggtc
    16321 ggctaacatt aaaaggtcaa tttgactttt tttatttatt tctatagttt ttatttctat
    16381 cataagttct ccaccctcac tttattcaaa ccttaattta acaaacttct tacagtactt
    16441 atttagttca ttttgatata tatttttact ttttaaaata tagtgataat tccctagaat
    16501 attaatacta ataataaatt ataatatcag ctctag
//
LOCUS       DAGSWI010000092        16518 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.092, whole genome shotgun sequence.
ACCESSION   DAGSWI010000092 DAGSWI010000000
VERSION     DAGSWI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.092"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..537
                     /gene="tuf"
                     /locus_tag="MJF80_002654"
     CDS             <1..537
                     /gene="tuf"
                     /locus_tag="MJF80_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBZ0281305.1"
                     /translation="VFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVE
                     MFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGG
                     RHTPFFDGYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFS
                     IREGGRTVASGVVATIIE"
     gene            787..936
                     /gene="rpmG"
                     /locus_tag="MJF80_002655"
     CDS             787..936
                     /gene="rpmG"
                     /locus_tag="MJF80_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBZ0281306.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            962..1183
                     /gene="secE"
                     /locus_tag="MJF80_002656"
     CDS             962..1183
                     /gene="secE"
                     /locus_tag="MJF80_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBZ0281307.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            1203..1745
                     /gene="nusG"
                     /locus_tag="MJF80_002657"
     CDS             1203..1745
                     /gene="nusG"
                     /locus_tag="MJF80_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBZ0281308.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1784..2209
                     /gene="rplK"
                     /locus_tag="MJF80_002658"
     CDS             1784..2209
                     /gene="rplK"
                     /locus_tag="MJF80_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBZ0281309.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            2280..2978
                     /gene="rplA"
                     /locus_tag="MJF80_002659"
     CDS             2280..2978
                     /gene="rplA"
                     /locus_tag="MJF80_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBZ0281310.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    3030..3158
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            3201..3707
                     /locus_tag="MJF80_002660"
     CDS             3201..3707
                     /locus_tag="MJF80_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBZ0281311.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3765..4130
                     /gene="rplL"
                     /locus_tag="MJF80_002661"
     CDS             3765..4130
                     /gene="rplL"
                     /locus_tag="MJF80_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBZ0281312.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            4522..5310
                     /locus_tag="MJF80_002662"
     CDS             4522..5310
                     /locus_tag="MJF80_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBZ0281313.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5781..9497
                     /gene="rpoB"
                     /locus_tag="MJF80_002663"
     CDS             5781..9497
                     /gene="rpoB"
                     /locus_tag="MJF80_002663"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBZ0281314.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9539..13024
                     /gene="rpoC"
                     /locus_tag="MJF80_002664"
     CDS             9539..13024
                     /gene="rpoC"
                     /locus_tag="MJF80_002664"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBZ0281315.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            13313..13735
                     /gene="rpsL"
                     /locus_tag="MJF80_002665"
     CDS             13313..13735
                     /gene="rpsL"
                     /locus_tag="MJF80_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBZ0281316.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13859..14329
                     /gene="rpsG"
                     /locus_tag="MJF80_002666"
     CDS             13859..14329
                     /gene="rpsG"
                     /locus_tag="MJF80_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBZ0281317.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            14378..16444
                     /gene="fusA"
                     /locus_tag="MJF80_002667"
     CDS             14378..16444
                     /gene="fusA"
                     /locus_tag="MJF80_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBZ0281318.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6485 a         2056 c         3383 g         4594 t
ORIGIN      
        1 gtattctcaa tcacaggaag aggaacagtt gcaacaggaa gagtggaaag aggagtacta
       61 aaagtacaag acgaagtaga attagtagga ttaacagaag caccaagaaa agtagtagta
      121 acaggagtag agatgttcag aaaattatta gaccaagcac aagcagggga taatatagga
      181 gcattattaa gaggagtaca aagaaacgag atagaaagag gacaagtact agcaaagact
      241 ggatcagtaa aggcacacac aaagtttaca gcagaagtat atgtacttaa aaaagaagaa
      301 ggtggaagac atacaccatt ctttgatgga tatagaccac aattctattt cagaacaaca
      361 gacgtaacag gagcttgtaa gttaccagaa ggaatagaga tggtaatgcc tggagataac
      421 gtaacaatgg aagtagactt aataaactca atagttgtag aagagggatt aagattctca
      481 ataagagaag gtggaagaac agtagcttca ggagttgttg ctacaataat agagtaattc
      541 tttactaaac tgatggatta aaaggaataa caaaaagagg ggttatgctc ctcttttttt
      601 aacatagaaa tacaaaaaca ttgacacagc ataggatttt gtgataactt atacaaggta
      661 atgattatag gaagttaata atatcttgaa tactgtagat ttttaataaa tacatatttt
      721 aaagatatta tattcacata tattaacaat atatattatt gtattacaaa gttggaggtg
      781 tatcagatga gagttaaagt aactttagca tgtacagagt gtaagcaaag aaactacaac
      841 actactaaga ataagaaaaa caatccagac agaatagaat tacaaaaata ttgtagattc
      901 tgtaaaaagc atacaactca taaagaaaca aaatagtttt ttataaggat gtgagtatgg
      961 aatggctgcc caaaaaaatg acggtgcaaa aactaaaaaa aggtttagtt tgtttggata
     1021 cttaaaagaa actaaacaag aattaaaaag agttacatgg ccgacaaaaa aggaactgtt
     1081 taaaaacaca agtatagttt taactgtagt tatttcatgc actatattag tatggggtat
     1141 agacactata ttatctggtg cactagcgtt attactaaaa tagacgaaaa gaggtaagtt
     1201 aaatgtcaga attacaagaa gcaagttggt atgtagttca tacctattcg ggacatgaaa
     1261 ataaagttaa agctacaatt gagaaagcag ttaaaacaag aggtatggaa gactgcataa
     1321 gacaagtagt tgttccaaca gaagaagtag tggaaactac taaaactgga aaagaaaaaa
     1381 ctagacaacg taaagtttat ccaagttatg ttttggttaa aatgataata actgatgaat
     1441 cttggtatgt agtaagaaat actaagggag ttacaggttt cgttggacct ggttcaaaac
     1501 cagtgccatt aagtgaagac gaagtcaaag ctatggggat agacacaaca gaccctaaag
     1561 tagtaaatag cgatattgat tttgaaatag gagatacagt aaaagtatct caaggaccat
     1621 ttagtggaca aataggtaac atagaagaaa ttgacttaga aaatagagaa gtcaaagtat
     1681 gcataaatgc atttggtaaa aggactctat ttgtaataga gcttgaaggt atagaaaaaa
     1741 tctaatacta tattatagaa aaatctgagg aggtgcgctc aaaatggcta aaaaagttat
     1801 aggtcaaata aaattacaaa tacctgcagg aaaggctact ccagcgccac cagttggacc
     1861 agcattagga caacatggtg ttaatataat gggattcaca aaagaattta atgctaaaac
     1921 tgcagatcaa gctggaatga taataccagt tgttataact gtatatcaag atagatcatt
     1981 cagttttata acaaaaactc caccagctgc agttttaatt aaaaaagcat taaacttaaa
     2041 atcaggttct ggagaaccaa acaagaaaaa agttgctaaa atgacttcag ctcaagtaag
     2101 agaaatagct gaattaaaga tgcctgactt aaatgctgca tcagtagaag ctgctatgag
     2161 tatgatagct ggtactgcta gaagcatggg tgttgtaatc gaagactaat taaaagatac
     2221 actttttttt ataaagtggg aggctagaag ccgaaattaa ccacgaggag gaaaataaaa
     2281 tggctaaaaa aggtaaaaga tatgcaggtg cattacaaaa agtagataga actaaatttt
     2341 atgatgcatc agaagcatta acattagttt cagatatagc tggagctaaa tttgatgaga
     2401 cagtagaagc acacattaaa ttaggtgttg actcaagaca tgctgaccaa caagtaagag
     2461 gtgcagttgt attacctcat ggaactggta aaacaaaaag agttttagtt tttgctaaag
     2521 gtgaaaaagc taaagaagct gaacaagctg gtgctgactt tgtaggagct gaagaattag
     2581 ttcaaaaaat acaaggtgaa aactggtttg actttgatat agtagttgct actccagata
     2641 tgatgggtgt agtaggtaga ttaggtagag tattaggacc taaaggttta atgccaaacc
     2701 ctaaatcagg aacagttaca tttgatgtag ctaaagctat agatgaaata aaagctggta
     2761 aagttgaata cagattagac aaaactaaca taatacacgt tccagtagga aaagtatcat
     2821 ttggtggaga aaaattaact gaaaacttta ctgcattaat ggatgctata ataaaagcta
     2881 aaccagctgc tgctaaagga caatatttaa gaagtataac agtagcttct tcaatgggac
     2941 ctggagttaa aataaaccca gcaagaacag ctgaataata aaactgttga cacaggttga
     3001 caaaaatgtt attatatcac ttgtcaatgt aaaaaagaat agatttgccg tagatagtgg
     3061 gtgcgagagc ttaatatccc gccgaggttt tagataaatt gagattacta aagcttttga
     3121 tgtctacttt tggcaacaaa agctttttat aatgttgcgg taaatactaa gcctatttgg
     3181 tgaaggaggt gcacatctat atgagaaaag ctatagaaat taaatcagaa gttgtttctg
     3241 agatagttga aaagttacaa aaatcttctg ctgctgttgt tgtagattac aaaggattaa
     3301 cagttgaaga agtaactgag ttaagaaaac aaatgagaga agctggtgta gattacaaag
     3361 tatacaaaaa tactttagtt agaagagcag ctaaagaagt tggtatagag caatttaatg
     3421 atgaattatt agttggaact aatgccatag catttggata tgatgatcca gttgctccag
     3481 ctagaatatt aaaaggattc atggattctc atccaaaaat gaaattaaag atgggtattg
     3541 tggaaggtgc attctatgat gaatctaaga ttgttgaaat ggctaacata ccatcaagag
     3601 aagttcttat cgctaaatta cttggtagct taaaagctcc agtatcaaac ttcgcatact
     3661 taatagatgc aatagcgaaa aaggcagaag gtcaagaaga agcttaattg cttaactaaa
     3721 aaattataaa tattaatttg aaataaaatt cggaggtgct aaaaatgaca atagaacaaa
     3781 tattagaagc tatagaaaat atgaaagttt tagaattaaa tgaattagtt aaagctgctg
     3841 aagaaaaatt cggtgtatca gcttcagctc cagtaatggt agctggtgca gctgctggtg
     3901 gaccagctgc tgaagaaaaa actgagtttg atgtagtatt aactgacgtt ggttcttcaa
     3961 aagttggagt tataaaagca gttagagaaa taacaggatt aggattaaaa gaagctaaag
     4021 aagtagttga caatgctcct aagacagtta aagaaggagc ttctaaagaa gaagctgatc
     4081 aaataaaaga gaaattagaa gctgctggag ctaaagtaga agttaagtag tttctttaaa
     4141 aagataccca ttgggtatct ttttttattt ataaaaaact ataaagaaag tatttggaat
     4201 tataaagaaa gtatttttat aaaataaaaa aaatttatat aatagtacta aattaagggt
     4261 atcatatatg gtgtaatggt attttaattt aacattatta tcaaattaaa atataaacaa
     4321 ttgttgtaat gtctgtatat aaagtatata attaaactaa aatgtaaaat tttacatttt
     4381 agtttaaaaa aatacacgag tgttttataa ataaaatgta ataaataatt aattaacaaa
     4441 tataaatttt aaaataagtt agatatttag aaaaatcgaa atttgtagga tttaaaggac
     4501 atattcaagg aggagttaat aatggaaaaa ttacaaggaa aaattgcagt agttactgca
     4561 gcaacaaaag gtattggatt agcatcagca gagatattag caaaaaatgg agcaactgtg
     4621 tacttagcag ctcgttcaga agaattagct aatgaagtta taaataaaat aagtgcagaa
     4681 ggtggttgtg ctaagtttgt ttactttaat gctcgtgagg aagagacctt tacctcaatg
     4741 atagaagagg tagttaaaaa agaaggtaag atagatatac ttgtaaacaa ttttggttca
     4801 acaaaccctt ctcttgacaa agaccttgta actggagata cagataattt ctttgacaca
     4861 gtaaatacta atttaaaaag tgtgtattta ccatgtaaag cagcaattcc tcatatgata
     4921 aagaatggaa aaggaagtat agtaaatata tcaagtatag gttcagtatt acctgattta
     4981 tctagaatag cttactgtgt atcaaaagca gcaatcaact cattaactca aaacatagct
     5041 acacaatatg ctaaagacaa tgttagatgt aatgctgtac ttccagggct tattgcaact
     5101 aaagcagcat tagacaatat gtctccagag ttcataaaag aatttttaaa gcatgttcct
     5161 ttaaatcgta taggggaacc agatgatata gcaaaagctg ttctattcta tgctagtgat
     5221 gactcatcat ttataacagg ggatttactt gaagttgcag gaggatttgg tttacctact
     5281 ccacaatttg cagataatat attaggataa ttaaattact aatatgcaat gaaagcttat
     5341 gcattagtta tagtagaaat gatattttta taaaggccat agatataatt tatattggaa
     5401 aggtttgagg ttcaaaattc caagttaaat ttatttatgg tctttatttt gtatatttta
     5461 ataaattgta tatatttgta taaatgtaat tatttacaat cattttaata tggaattata
     5521 attaaatagg ggggatggta cttgtataaa attttttata gaaaattaga cctataaatg
     5581 aaaaatatgg attttatatt ggaaaattgt acataataca taatatatgc ttaaaataat
     5641 ttacaaaaaa tcaaaaataa tgcatagttt acatataaga taaaaaatat attgaaaaat
     5701 acttgacatt atgctaaaat tattagatgc gttagaatga actattgttt tatttttaat
     5761 tattgagagg tgaaaagtga atgccacatc ctgtcacgat aggtaaaaga actagaatga
     5821 gcttttctaa aattaaggaa atagctgatg tgccaaatct tatagaaatt caagtagatt
     5881 cctatgagtg gtttttaaaa gaaggtttaa aagaagtgtt tgatgatatt tcaccaatag
     5941 aagattatac tggtaatctt atattggaat ttgtcgacta ttctctagat gataaaccaa
     6001 agtacgatat agaagagtgt aaagagagag atgctacata ttgtgcacct ctaaaggtta
     6061 aggtaagact tataaataaa gaaacaggtg agataaaaga acaagaagtg tttatgggtg
     6121 acttcccttt aatgacagaa agaggaactt tcgttataaa tggagcagaa agagttatag
     6181 taagtcaatt agttagatct cctggtgttt actatgcgga agaaagagat aaaacaggta
     6241 agagattgat ttcatctact gtaattccta atagaggagc atggttagaa tatgaaacag
     6301 attctaatga tgtaatatct gtaagagttg atagaacaag aaaacaacca gttacagttt
     6361 tacttagagc tttaggaata ggaacagatg cagagataat tgacctttta ggagaagatg
     6421 agagattatc tgctacatta gaaaaagata atactaagac agtagaagaa ggtcttgtag
     6481 agatatacaa aaaattaaga ccaggtgaac ctcctactgt agaaagtgca tcatctttat
     6541 taaatgcttt attctttgac ccaaaaagat atgacctagc aaaagttggt agatataaat
     6601 tcaataagaa acttgcttta tgttatagaa taatgaataa aatttcagca gaagacataa
     6661 taaatccaga aactggagaa gtatttgtaa aagctggaga aaaaataagc tatgatttgg
     6721 ctaaagccat acaaaatgca ggtataaatg tagtaaatct actaatggat gatgataaaa
     6781 aagttagagt tataggaaat aattttgtgg atattaagtc tcacattgat tttgacatag
     6841 atgatttaaa cataaaagaa aaagtacatt atccaacttt aaaagaaatt ttagatggat
     6901 atagtgatga agaagaaata aaagaggcta taaaatcaag aattaaagaa cttataccaa
     6961 aacatatatt attagatgat ataatagctt caataagtta tgagttcaat atattctata
     7021 atataggaaa tattgatgat atagatcacc taggaaacag aagaataaga tctgtaggtg
     7081 aattgttaca aaatcaagtt agaattggtc tttcaagaat ggaaagagtt ataaaagaaa
     7141 gaatgacggt tcaagatatg gaagctataa cgcctcaagc gttagttaac ataagaccag
     7201 tttcagctgc aataaaagaa ttttttggta gttctcagtt atctcagttc atggatcaaa
     7261 caaatcctct atctgagtta acgcataaga gaagattatc agcccttggg cctggaggtc
     7321 tttcaagaga aagagctgga ttcgaagtgc gtgacgttca ccattcacac tatggtagaa
     7381 tgtgtccaat agagactcca gagggaccaa atatcggtct tataaactct ctaggaactt
     7441 atgcaaaaat aaatgaattt ggatttatag aatcaccata tagaaaattt gataaagaaa
     7501 catcaacagt tactgatgaa atacattatt taactgctga cgaagaagat ttattcgtaa
     7561 gagctcaggc aaatgagcca ttaacagaag atggtaaatt cgtaaaccat agagttgttt
     7621 gtagaactgt aaatggtgct gttgaaatgg ttcctgaaag tagagtagat tacatggata
     7681 tatctcctaa gcaggttgta tctgttgcca ctgctatgat acctttctta gaaaatgatg
     7741 atgccaaccg tgcccttatg ggagcgaaca tgcagcgtca ggcagtgcct ctagttagaa
     7801 gagaagcacc aattatagga actggtatag aatacagagc agcaaaagac tctggagcag
     7861 ttgttgttgc tagaaattct ggtatagcag aaagagtaac agcagatgaa ataattataa
     7921 aaagagaaga tggaaacaga gatagatata atctacttaa gtttaaacgt tcaaactcag
     7981 gtacttgtat aaatcaaaca cctataataa ataagggtga ccagataata aaaggtgatg
     8041 ttatagcaga tggtccagca actgatttag gagaagttgc acttggaaga aactgtctta
     8101 tagcattcat gacttgggaa ggttataact acgaggatgc tatattaata aatgaaagat
     8161 tagttaaaga agatagatta tcaacaattc atatagaaga atatgaatgt gaagctagag
     8221 atacaaaact agggccagag gaaataacta gagatatacc taatgttgga gatagtgcaa
     8281 ttaagaactt agatgacaga ggtataataa gaataggtgc agaagtagac tcaggtgata
     8341 tattagttgg taaagtaact ccaaaaggag aaactgaact tactgctgag gaaagattac
     8401 ttcgtgcaat atttggagaa aaggctagag aagttagaga tacttcactt aaagtgcctc
     8461 atggtgaatc tggtataata gttgatgtaa aagtatttac tagagaaaat ggagatgatt
     8521 tatctccagg agtaaatgaa ttagtaagat gctatatagc taaaaagaga aaaataaaag
     8581 ttggagataa aatggctggt cgtcatggta ataaaggggt tatctcaaga gtattacctg
     8641 aagaagatat gccattcatg gaaaatggaa caccactaga cataatactt aacccacaag
     8701 gtataccatc acgtatgaac attggtcagg ttttagaagt tcatttagga cttgctgcta
     8761 agacattagg atggtatgta gcaacatcag tattcgatgg tgctaatgag tatgacataa
     8821 tggatgctct tgaagaagct gggtatccta gagatggtaa attaacttta tatgatggta
     8881 gaacaggtga gtcttttgac aacagaataa ctgttggata tatgtactac ttgaaactac
     8941 atcacttagt agatgaaaaa ctacatgcaa gaagtactgg accatactca ttagttactc
     9001 aacagccact aggtggtaaa gcacaatttg gtggacaaag atttggagag atggaggttt
     9061 gggctctgga agcatatgga gctgctcaca tacttcaaga aatacttact gttaagtctg
     9121 atgacgttgt aggtcgtgtt agaacatatg aagctatagt taaaggtgaa aatatacctg
     9181 aaccaggaat cccagaatca tttaaggttc ttataaaaga acttcaaagt ttatgcttag
     9241 atgtaaaagt attgacagat gaagaccaag aaatagaagt aagagaatct gtggatgaag
     9301 atgatacaat aggtgaattt gagctagatg ttgtaaatca tatgggagaa gttgaagaaa
     9361 gtaatataat agaagaaatt gaagatgatt ttgcagaaaa tgcagaagat gaggatatag
     9421 agaatttaga agaattcact gaagatgatt tatttgaaga agaaatagat tttgatagtg
     9481 atgactttga tatgtaaatt taattaatgt aaatctcaaa tagaagggag agaactcctt
     9541 gtttgaatta aacaatttcg agtcgataaa aatagcattg gcttctccag aaaaaataag
     9601 acaatggtct aggggagaag ttaaaaagcc agaaactata aattaccgta ctttaaaacc
     9661 agaaaaagat ggtcttttct gtgaaagaat atttggacca caaaaagact gggagtgcca
     9721 ctgtggtaaa tatagaagag ttagatataa aggtgtagtt tgtgatagat gtggagtaga
     9781 agtaactaaa tcaaaagtaa gaagagagag aatgggacat atagagctag ctgctcctat
     9841 gtctcacatc tggtacttca aaggtatacc aagtagaatg ggacttttac ttgatatgtc
     9901 accaagatcg ttagaaaaaa tattatactt tgcctcatat gtggtagttg atccaggaga
     9961 gactggatta aacgaaaaac aattgcttac agaaaaagaa tacagaactg ctcttgaaaa
    10021 gtatggatat acttttactg taggaatggg tgctgaagct gtaaagacat tactacaaaa
    10081 tatagattta gagcaacaaa gtaaagacct aagagcggag ttaaaagata gtacagggca
    10141 aaagaaagtt agaacaataa gaagattaga agttgtagag gcatttaaaa agtctggaaa
    10201 taaaccagaa tggatgattt tagatgcaat accagtaata ccaccagatt taagaccaat
    10261 ggtacaactt gatggtggaa gatttgcgac ttcagaccta aatgatttat atagaagagt
    10321 tataaataga aacaatagac ttaaaagatt attagagctt ggagctccag atataattgt
    10381 aagaaatgaa aaaagaatgc ttcaagaagc tgttgatgca ttgatagata atggtagaag
    10441 aggtagacct gtaacaggac ctggaaatag accacttaaa tctttatcag atatgttaaa
    10501 aggtaagcaa ggtcgtttcc gtcaaaactt acttggtaag cgtgttgact actcaggacg
    10561 ttctgttata gttgttggac cagaacttaa attttatcaa tgtggacttc caaagaagat
    10621 ggcattagaa ttattcaagc catttgttat ggataaatta gttaaagaag gttatgcaca
    10681 taatataaaa agtgcgaaat ctatagtaga aaaagttaag ccagaagttt gggatgtttt
    10741 agaagatgtt ataaaaagtc atccagttct tcttaaccgt gcaccgactc tacatagatt
    10801 aggtatacaa gcatttgaac caatcttagt tgaaggtaag gctataaaac tacatcctct
    10861 tgtatgtaca gcttataatg cagactttga tggtgaccaa atggcagttc atgtaccttt
    10921 atcagtagaa gcacaagcag aagcaagatt cttaatgctt tctgtaaata atatacttgc
    10981 tcctaaagat ggttcaccta taactactcc atctcaggat atggttttag gttgctatta
    11041 tctaacaata gaagcccaag atggagctaa aggaacaggt atggtattta aagactttaa
    11101 tgaactatta cttgcttatt acaataaatc agttcatcta catgcattag tgaaacttaa
    11161 ggtaacactt gaagatggaa gaagttcatt ggttgaaagt actgttggta gatttatatt
    11221 taatgaaaat atacctcaag acttaggttt tgtagataga aaagaaaatc catttgcact
    11281 tgaagttgat ttcttagcag ataaaaaatc tcttggtaaa ataatagata aatgctttag
    11341 aaaacacgga aatacagaaa cagctgaatt actagattat ataaaagctc taggatttaa
    11401 atattctaca ttaggtggca taacagttgc tgttgatgat atgagtgtcc cagaagaaaa
    11461 gaaagtattt atagctgagg cagaagctaa agttgataag tatgaaaaag catacagaag
    11521 aggtctaatc tctgatgaag aaagatatga aaaagttata gagacatgga cagaaacaac
    11581 tgataaagtt actgatgctc ttatgggtgg actagataga ttaaataata tatatataat
    11641 ggcacattca ggagccagag gttctaaaaa ccaaattaga cagctggcag gtatgcgtgg
    11701 tcttatggca aatgcatctg gtaaaacagt tgagatacca gttaaatcta atttccgtga
    11761 aggtttatca gtactagaat actttacatc ttcacatggt gccagaaaag gtcttgccga
    11821 tacagctata cgtacagctg aatctggata tttaacaaga agacttgttg atgtaagtca
    11881 agatgttatt gtaagggaaa tagattgtgg aacagaagat actacagaga tttatgctat
    11941 aaaagaagga aatgaagtta tagaagagat atatgataga attgtaggaa gatatactat
    12001 agaccctata ttaaatcctg aaactggaga agttatagtt gaggctgatt caatgataca
    12061 agaagatgaa gcagaaacta tagtagcttt aggaattgaa aaaattagga taagaacagt
    12121 tcttaactgt aaaactaatc atggagtttg ttctaagtgt tatggtagaa acttggcaac
    12181 aggaaaagaa gttaatatag gtgaagcagt tggtataata gcagctcaat ctatcggtga
    12241 gccgggtact cagcttacaa tgcgtacatt ccatactgga ggagttgcag gagctgatat
    12301 aactcaaggt ctaccaaggg ttgaagaatt atttgaagct agaaaaccaa aaggattagc
    12361 tgtaataact gaagtatctg gtagagttga aattgatgaa acaggaaaga gaaaagaagt
    12421 aaatgtaata ccagaagaag gcgagactca aacatatgta attccatatg gttctagatt
    12481 aaaagttaag caaggtcaaa tgctagaggc tggagaccct ctaacacaag gatttataaa
    12541 tcctcatgat atagtaagag taaatggtgt taagggagtt caagaatata tagttaaaga
    12601 agttcaaaga gtgtatagac ttcaaggggt tgatgttaat gataagcata tagaagttat
    12661 agtaagacaa atgctatcta aagtaaaagt tgaagaccca ggagatacag atttattgcc
    12721 aggtggatat gaagatgtat taacattcaa tgaatgtaat aaagatgcta tagataaagg
    12781 tctaagacca gcagttgcta aaagagtttt acttggtata actaaagcat ctcttgcaac
    12841 tgattcattc ttatcagcag cttctttcca agaaacaaca agagtattaa cagaggcagc
    12901 aataaaaggt aaagaagacc acttaatagg acttaaagaa aatgttatat taggtaagtt
    12961 aataccagca ggaacaggaa tgaagaaata tagaaatata gctgttgaaa aaattgaaga
    13021 ttaatagcat atagagtaaa gaaatcttga caaccattat ttgtggtgtt aaaatagtaa
    13081 agtgtgttgt ttaaaagtac actaagggct tgaacaagtt cttagtgact tttagatggc
    13141 aataaatgag aactaaaaag catcaagcta gaaaagtgct aatatcacat atttatgtgt
    13201 tatagacatt aaattgtaag taacgaatgt tacaatcagt cagtatgaat attcatattg
    13261 atgtagttgg cggtcactga taagatcgaa aatttggaag gaggtgcaga tgatgccaac
    13321 aattaaccaa ttagttcgta aaagtagaaa agcgctagag aagaaatcta ctgctccagc
    13381 attacaaaaa ggttacaact ctttaaataa gaaagtaact gacgctagtg ccccacaaaa
    13441 aagaggggtt tgtacttcag taaaaacagt tactcctaag aagcctaact cagctttaag
    13501 aaaagttgcc agagttagat taactaatgg tatagaagtt tctgcttata taccaggaga
    13561 aggacacaac ttacaagagc atagtgttgt tcttataaga ggaggaagag taaaagacct
    13621 tccaggggtt agataccata tactaagagg tactctagat acagcaggtg ttgataagag
    13681 aagacaatca agatctaaat acggtgcgaa gagacctaaa gaagctaaaa agtaaaaaca
    13741 attaatgatt gattatagtt cggtgcttta aatatatata tatttacggc atcacggcat
    13801 taaatgattt atgtcgagta cctatgattt aagttaaata attaaggagg gaagcgaaat
    13861 gccaagaaaa ggtaatattc caaaaagaga agttttacca gatcctatgt atgggagtaa
    13921 ggtagttact aaattaataa ataatctaat ggtagacgga aaaaaaggaa agtctcaaag
    13981 aatagtttat gatgctttcg caattgttgc tgaaaagaca ggagaagaag ctttagaagt
    14041 atttaataaa gcaatggata atataatgcc tgttttagaa gtaaaagcaa gaagagttgg
    14101 tggggcaaac taccaagtgc caatcgaagt tagacctgaa agaagacaaa cattaggttt
    14161 aagatggcta gtaaaatata ctagagctcg tggagaaaaa ggaatggttg aaaagctagc
    14221 taaagagata atggacgcag ctaacaacac tggagcttca gttaagaaaa aagaagatac
    14281 tcataaaatg gcagaagcta ataaagcatt tgctcattac agatggtagg atatatagat
    14341 atcttataaa attgaggata tcataaggag gataaccatg gctagaaagt ttcctttgga
    14401 aagaactagg aatataggaa tcatggctca tatagatgca ggaaaaacta ctactacaga
    14461 aagaatcctg ttttatactg ggcaaactca taaaatagga gaaactcatg aaggagcttc
    14521 tcaaatggac tggatggagc aagaaaagga aagaggtata acaataactt ctgccgctac
    14581 tactgcttca tggaaagacc atagaataaa cataatagat actcctggac acgtggactt
    14641 cacagttgag gttgaaagat ctctaagagt tcttgacggt tcagttgctg tattctgtgc
    14701 aaaaggtggg gttgagcctc aatcagaaaa tgtatggaga caagctgaaa cttatggagt
    14761 acctagaata gcttttgtaa ataaaatgga tatattaggt gcagatttct acaatgttgt
    14821 aagcatgatg aaatctagat taaattctaa tgcagttcct atgcaattac caataggtaa
    14881 agaagattca tttataggta taatagactt attaaaaatg gatgctgtta tatacaaaga
    14941 cgacctaggg gttgaaatgg aagaaacaga tataccagaa gatatgaaag aattagctgc
    15001 tgagtggaga gaaaaattag ttgaatcagt agctgaaact gatgaagaat taatgatgaa
    15061 ataccttgaa ggtgaagaac ttactataga tgaattaaaa gttgctataa gaaaagctac
    15121 tatagcttgt gaaatgaacc cagttttctg tggtacagct tatagaaaca aaggtgttca
    15181 gttagtaata gatgcagttt tagattactt accagcgcca actgatatac cagctataaa
    15241 aggtatatta gcagatggag aagaagctga aagacattct tctgatgaag agccattctc
    15301 tgcattagca ttcaaaataa tgactgaccc attcgttgga aaactagcgt tcttcagagt
    15361 ttattctgga acattagaat caggttctta tgttttaaat gcaactaaga ataagagaga
    15421 aagaattggt cgtatattac aaatgcatgc taatacaaga gaagaaataa ctaaagtgta
    15481 tgctggagat atagcagcag cagtaggatt aaaagatact actactggag acacattatg
    15541 tgaccctgct aatccaataa tacttgaatc tatggaattc cctgagccag ttatatctgt
    15601 tgctatagaa ccaagttcta aagcagctca agaaaaaatg ggtatagctc ttcaaaaatt
    15661 agctgaagaa gacccaactt tcacagttaa gactgaccaa gaaacaggac aaactataat
    15721 atctggtatg ggtgaattac acttagagat aatagtagat agattattaa gagaatttaa
    15781 agtagaagct aaagttggtg caccacaagt tgcatataga gagactataa ctcaaccagt
    15841 tgatgttgaa tacaagtact ctaagcaatc aggtggtaga ggacaatacg gtcacgttaa
    15901 gattagagtt gctcctcaag agccaggtga aggatacaaa ttcacaaaca aaactgttgg
    15961 tggatcagta ccaaaagaat atgttggtcc agttgacatg ggtatacaag gagctatgca
    16021 atctggtata gttgctggat atccagttgt agacgttgct gttgagttat atgatggttc
    16081 ttaccatgag gttgactctt ctgagatggc gtttaagatg gctggttcaa tggcattcaa
    16141 agatgctatg aagaaaggta atgcagttct tttagagcct tacttcaaag tagaagttgt
    16201 tactccagaa gattacatgg gagatgttat gggtgactta aactcaagaa gaggattaat
    16261 ccaaggtatg gaagctagaa gtggtgcaca agttataaat gcattcgttc cactttcaga
    16321 aatgtttgga tactcaacag acctaagatc ttcaactcaa ggtcgtgcaa cttacacaat
    16381 gatattcgat cactatgagc aagttccagc ttcagttgct aagaaaatag ctgaaggaaa
    16441 ataatttaaa aataattaat aatataaata taaaaaataa tcagctagat gagagatatc
    16501 tctcatctag cacaagat
//
LOCUS       DAGSWI010000093        16096 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.093, whole genome shotgun sequence.
ACCESSION   DAGSWI010000093 DAGSWI010000000
VERSION     DAGSWI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.093"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(118..804)
                     /locus_tag="MJF80_002668"
     CDS             complement(118..804)
                     /locus_tag="MJF80_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBZ0281319.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(853..1386)
                     /locus_tag="MJF80_002669"
     CDS             complement(853..1386)
                     /locus_tag="MJF80_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBZ0281320.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(1430..2062)
                     /locus_tag="MJF80_002670"
     CDS             complement(1430..2062)
                     /locus_tag="MJF80_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBZ0281321.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2152..3231)
                     /locus_tag="MJF80_002671"
     CDS             complement(2152..3231)
                     /locus_tag="MJF80_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBZ0281322.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3364..4710)
                     /gene="pepV"
                     /locus_tag="MJF80_002672"
     CDS             complement(3364..4710)
                     /gene="pepV"
                     /locus_tag="MJF80_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBZ0281323.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELNKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4779..6059)
                     /locus_tag="MJF80_002673"
     CDS             complement(4779..6059)
                     /locus_tag="MJF80_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBZ0281324.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6186..6446)
                     /gene="hfq"
                     /locus_tag="MJF80_002674"
     CDS             complement(6186..6446)
                     /gene="hfq"
                     /locus_tag="MJF80_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBZ0281325.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6560..7495)
                     /gene="miaA"
                     /locus_tag="MJF80_002675"
     CDS             complement(6560..7495)
                     /gene="miaA"
                     /locus_tag="MJF80_002675"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBZ0281326.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7533..9500)
                     /gene="mutL"
                     /locus_tag="MJF80_002676"
     CDS             complement(7533..9500)
                     /gene="mutL"
                     /locus_tag="MJF80_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBZ0281327.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9511..12354)
                     /gene="mutS"
                     /locus_tag="MJF80_002677"
     CDS             complement(9511..12354)
                     /gene="mutS"
                     /locus_tag="MJF80_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBZ0281328.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIENLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALIKQNDDKEKINQVDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(12514..13248)
                     /locus_tag="MJF80_002678"
     CDS             complement(12514..13248)
                     /locus_tag="MJF80_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281329.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(13217..14230)
                     /locus_tag="MJF80_002679"
     CDS             complement(13217..14230)
                     /locus_tag="MJF80_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBZ0281330.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSLSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(14252..15022)
                     /locus_tag="MJF80_002680"
     CDS             complement(14252..15022)
                     /locus_tag="MJF80_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281331.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(14997..15287)
                     /locus_tag="MJF80_002681"
     CDS             complement(14997..15287)
                     /locus_tag="MJF80_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBZ0281332.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(15418..15517)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         5041 a         2789 c         1663 g         6603 t
ORIGIN      
        1 atttatacta taattattaa tttgatgata aaagctattt tccttctaaa taaaaaattt
       61 gtctaaagtt tctaaaaaat ttagacaaat cttaggttat tactctttaa attattttta
      121 aattattcca aatacgccaa ataatattgg tataaatata gctggtaaca aattggctac
      181 ttttatattc gttaatttaa gtacatttag accaagtcct ataattaata aacttccagc
      241 tgctgtcatt tctgtgacta caggatcact taaaaatcca tttaaaaatg atgctcccac
      301 acatataata ccttcataca aaaatacaga tattgcagaa aacattacac ctatacctaa
      361 agaagcagta aatataattg aagctattcc atctataaca gattttacaa ataaagtatc
      421 atgacttcct gtaagaccac tttctaatga accaactact gccatggctc ctatacagaa
      481 taataaacta gatgatataa aaccctctgc tatagaatct tttttatttc ctttagaaaa
      541 tttactttgt aaaaaagcac ccaacttatt tacccatttg tcaatatcta ttaattctcc
      601 tatcagagca cctacagcta cggatattat tgtcactaat gtgttttttc cttctagtgc
      661 tcctgatata ccaatgtaca atacacataa agcaattcca ttcattatag tatcgcttac
      721 tctttgaggt aatcctcctt ttattataag acctactata catccacctg caattgctag
      781 actatttgct attactccaa acattttttc tcccctcgat tagctaattg aaatttatcc
      841 tattattaaa acctaaacat ctatactttg tattggatat tttaaatatt ccaaaaattc
      901 ttcaaaacct tttctagata tatttacagt tttagtattt atgttaggat gaaaatttaa
      961 gtaatcttct ttaattacat ctttatctat gtaaagctca acttttcttt ctgtatcatt
     1021 tattaatgaa aaaggattta cacttccagg aagaagttta agtacattgt atagtctttc
     1081 aggtgaagca aacgataatc tagaacttcc tatcttatct ttaagtgagt ttaaatcaac
     1141 ttgtttttct cctttaacta caataagata atacttattt cctttggcat ttcttaaaaa
     1201 taaattttta cattgacacc cctttgttat tccatctaaa atgactaatt gctctgctgt
     1261 atatacagct tcatgctcta ctacatcata ttcaacattt aatttatcca aaatatcata
     1321 tattttagtt tcattcatgc tagattcatt ttcatcttgt tcttttttat tatttaaatc
     1381 actcatactt ttctccttaa acacttcatc ataatatcat tgtgaggtat tattctacta
     1441 tagctattgc tcttactgga gctccatcag catcattata cttaaatggt gctgctataa
     1501 atagaaattt ttctggaaca ttttctaaat ttgtaagatt ttctatgatt atttttcctt
     1561 tttcaaataa aatatgatgc acttcaaata ctgatgtgtc atatctatcc actgatagca
     1621 tatctattcc tatgcctttt atattagtat tagctatata gtttgcagct tcttttgtta
     1681 atgtaggata acctacatag tactgctttt tatcccaaaa ttttgaccaa cctgatttaa
     1741 atattataaa atcactattt tttatttcat cttcatattt ttttagatat tctaattcta
     1801 tattctcatt tcccttttca agttccaata cataagcttt cccaacaaaa ttttctatat
     1861 ccaacttatc tagggtctca cctttagtat acatatgtct tggtgaatcc atatgagttc
     1921 cattgtgaga aaatacacta attaatgttt cttgataacc attttcttca ataatagcta
     1981 ctttcttgat atctggtcta ttaggctctg catatacagg catgtcatta tgtgtcacat
     2041 gagttaaatc aaaaaccttc atctttatat accccctatg tttttatttt ctattatata
     2101 cctaaattca ttttatttct atcagatttt attccattat ataccatact actatatatt
     2161 ttttataaac ctcaagggat ttaccgaaaa atcttggaaa gaattatcag taaatataac
     2221 atcttcaccc aaaaaacgtt tataccttcc ttcttctttc tttaaagtaa ttctctgttt
     2281 ttgtgaatga ttattaaaaa ccattatctt aagaggaggt ttttttctat tagtctgcac
     2341 tctttttaac atgtatttta tgtgcatatc agcttcagga aatgaatatc cacaaaatat
     2401 taaaaggtca gtatctctta aagttttttc tgtttcattc caaacattac ttaaaaatat
     2461 gtttgacata ttcttaaaat atgttggtgg cactataata ggttctgtta actcaccaca
     2521 ctctaaacat tttgtctctg aactattttc aataagcttc atgacaccac cttcatgtgg
     2581 tgtaagagtt acactattac aaatggggca ataaagccaa ttcaatgacc catgtatctt
     2641 atataatttg attaatggtt cttttggttt tctccaattt aactcctctt taaaatttgt
     2701 aaactccaca ccataatcaa gcataattgg caaatcattc ttatctctta atccagtaac
     2761 tgtattgtct atatgtatat cataatttgc actaataaaa gtagtatcaa gaagcaaatc
     2821 attttccaaa agattttcaa ctaaaagttt atgatattta ttatctgttc taggtgtatt
     2881 atgaattgct tcagccatta gcagtattaa atattgtctt aaaaaacgta tactatcact
     2941 tctgttattt agattttcta gtccaaaatt tctaaatgct tcttttcttt gttctgctaa
     3001 gtctaaaaga cctaaaactt cttcaaaggt aggaaaattt gcattatcta tattatcttt
     3061 tactatatca atattgaaca tctgtttaaa gaaaatatac aattctctat tcattttggt
     3121 attataattt gtgggattta gttttttaaa atattcgcta aaaagttcat tttgaatagg
     3181 tagattctct gctgctgaag ctcctgctcc taaaaatatt gctattttca tatagttcct
     3241 cctaatataa atattactcc tatttttata tccattaaat atccatatca tttattttat
     3301 gatacaacaa taaagctgtc ttttactaga aaaaatccta ttacaagaca gccttatatt
     3361 atattaaatc tttaataatt cgtaaataga aagagcatat atttgagtag cttttataat
     3421 atcctctact attaagtatt cattagcttg gtgttctgtt tctggttttc ctggaaaaat
     3481 agctccaaat gcaacacaat tatctaaagc tcttgcgtat gttgctcctc cagatgaaag
     3541 tggtgttcca tcaagaccag tttcttcttc atatactttt ctaagtgtct taacaagaag
     3601 agtatcttct ggaacataga ttgaatccaa gaaatcatat tcttcataat ttaaattgta
     3661 tttatctgtc attttcttta actcttttac aaaatcatcc tttttgtatg ttactggaat
     3721 ccttacatct attcctgcaa attctttttc gttatcaata gttactctac ctatatttac
     3781 tgttagttta cctgatacat catccttgca gttaggaatt atattgtttc cattagcatc
     3841 ttcaccaata acttctgcta aaaacttaat tatattagaa tctataccta tattattaag
     3901 agctatacaa agtctagcca ctgcatttat accagtatca cttgctgctg aatggacact
     3961 ctttccaatt atacagattt tattaccttc aacagtatac tcaaaattta atttattaag
     4021 ttcttttttt aatttatcag agtattttcc aatatagatt gctttacctg gaactgcatt
     4081 taaagccttt ccaactgaca actctatatc acttttgcta tcacaagtca aatgaacttg
     4141 taaaagacct ttctctgcat ttgttattgg gaatctagaa tctggagtaa atccatagtt
     4201 tggtatttct tcattatttt ctttatattt atttatacat ctccaaagat tttcttcatc
     4261 agccccaaaa atgaatctta tcctcttatt aaagtctaca tttaaatcca taagagcttt
     4321 cactgcataa attgctgata ttgtaggtcc tttatcatct tgtgttcctc ttccatacaa
     4381 tttaccatct tctaaaacag cttcaaatgg agggtaattc cagctttcta aatcaccttc
     4441 tggaaccaca tcaacgtgac caagtatacc tatcattttt tcaccttgtc ctatttctgc
     4501 atatccataa tatccatctt tgtatactgt cttaaatcct aaagatttac ataaatctaa
     4561 tatacctctt aaagctttat ctacattttc accaaatggg caattttcag ttgcttcaga
     4621 tataacacta ggtattttta cactttcttg tattgaagaa atcatttcat cttgtagaga
     4681 atttactttt tctttaattt gctctttcat attatcaccc ttcaattttc atcataatat
     4741 actaataaat attttgacct tttaaaaaaa aaatatgctt attttttcag tttttttata
     4801 aattcaatcg cttttaaagt ccccattttt gaatggtcaa atgtaagtcc accttggaaa
     4861 taaacattat aaggaggtct tattggagca tctgcactta attctataga agaaccttgt
     4921 ataaatgctc ctgctgccat tatgacttca ctttcgtatc caggcatgtc ccaaggaaca
     4981 ggttttacat aagaatcgac tggtgcagct tcttgtatac cttcacaaaa acttattact
     5041 tcatcagcat tattaagtct aatacattga ataatatcac ttcttaagtc atcatatttt
     5101 ggtaatacat catatcctaa tttttcaaaa gctcttgagc aaaagatagc tcccattaca
     5161 gcctgagata ctatataagg cgccaagaaa aaaccctgaa gtacatttct agttgttcca
     5221 aaagtaagac cacattcttt tcctattcca ggagaagtca ttctataaga tataagctca
     5281 atcaaatctt ttctaccagc tatatatcca cctgtcaatg caagcccacc accaggattt
     5341 tttattaaag agccagccat aacatctgct ccaacatcag taggctcttt ggtatctaaa
     5401 aactcaccat aacaattatc aaccattact atagcttctg gtttaacaga ctttattact
     5461 tctatagctt cttttatatc actaattgaa agtgattttc tccaagaata tccttttgac
     5521 ctttgtatca taacgagttt agttctatca tttattttgt ttttaattcc ttctaaatct
     5581 aagtttccat cctctaaaaa atctacatca tcatatgtaa caccatactc tttcaatgaa
     5641 cctttttctt ctcttatgcc tataactccc tctaaagtat catatggtct accagtaaca
     5701 gataaaattt catctcctgg tctaactata ccttgtatac ataaagtaag tgcatgtgta
     5761 ccatttacaa ttattggtct tacaagagca tcttctgtat taaatacttt agaataaatt
     5821 tcctctattt tttcacgacc aatatcatta tatccatatc ctgtagtcca gttaaagtgc
     5881 atatcactta gtttagattc ttgcatagct tttaaaactt tatattgatt atattctctt
     5941 atttctttta tttcttcaaa cttatctttt atttcttcca ttatttcttg cgatagttta
     6001 aaagtatcat catctattcc atagtagtct tttaataatt cttttgtctc attaagcatt
     6061 ttatcacctg tctctttatt attttattta aacatgtaaa ggagactatt ttactaaaac
     6121 aaataaatta tagtttataa gttagtataa gtctcctctc aatcatctta aattaaatta
     6181 attatctatc tgttgttatt attattgttg ttttggtttt gatttgttag attaatatac
     6241 tttccaggtt gtatagttga tacagcatgt ttataaatca tgttttgttg tctattatcc
     6301 ccttctatta atattatata actatcaaac ccctttacaa gccctttaac ttgcactcca
     6361 ttaactaaat atatagtaac aggtatcctt tctttccttg cattatttaa aaacaaatct
     6421 tgtaaattta aaactgtatt tttcattaga tttgtaccct cccaagatta aattgaactt
     6481 ctaaaatatt cctaccttat attctatata agttatgaca aattttcaat attaagttgt
     6541 atacttaaaa atttatatat tattttatag aatctttaat atataagatt atttcatttt
     6601 ttaattcgtc tatattttca tattggtcta aatcaaacca ttttgctgtt ttatatcttt
     6661 taaaccatgt tatctgacgt tttgcatacc ttctagaatc tcttttaatt atttcaatag
     6721 cttcttcgta tgtatattct ccatccagat actttaatat ctctttataa cctatacctt
     6781 gcattgatat catatctttt ttaaatccca tacttaaaag tttttttacc tcttcaacca
     6841 atccattttt aagcataata tcaactctca tatttattct ttgatacaaa tgttctctat
     6901 ctctatttag aactattatt attggttgat attcttcatt aaattttaaa tcacttgaaa
     6961 aatcattcat tttcttacca cttagacaaa cttcaagagc tcttatgacc cttttagtat
     7021 tgtttttatg gattctatta gcagattctt catcaagttc ttttaactta ttatgcatgt
     7081 aatctattcc cttttcagca agttgttttt gaagttcttc tcttaattca ttattggcat
     7141 cagattgtgc aaaatccata ttatatatta aagagtttaa ataaagtcct gtgcctccag
     7201 ttactaaaac acatttttcc tttttattta tttcatgtat atattttttt gctctttgtt
     7261 gaaactctga aactgaaaat ggatagtcag gcttaacttc atctataagg taatgcttca
     7321 ctccttgcat ctcttcctct gtaacttttg cacttccaat atccatatat tcatatattt
     7381 gcatagaatc agctgaaatt atctctgcat tcatatcttt tgctaatttt attgacaaat
     7441 ctgtttttcc aacagcagtt ggacctgtaa gtattataag tggtattttt ttcataacat
     7501 tcctttccat atattttaaa tatccaaata ttttacataa ttcttttaaa cattttttct
     7561 atttctgtct tagatatttc gaccattata ggtcttccat gaggacatgt aaatggattc
     7621 tcacactttt ctagctgttc caataaactt tttatttcaa tatcgtatat tttatcattt
     7681 gcttttatag cagacctaca tgccatagat gcaaatctct ctccttttaa atcataatta
     7741 cttgtaattt cttctatatt atcaattatt tgaagtataa atttctcggt ttcaggtact
     7801 ccaaatatag taggaacaca tcttaccatt atatgattat tgccaaaaat ttctagctca
     7861 aaaccaaatt tcataaataa ctccaagtta ttttcaattt gaagcatatc tacatttgat
     7921 acttcaataa ccactgggtc taaaagtatt tgcatattta tatcttgtct atagaacttc
     7981 tccatatatc tttcatatag tattctttca tgtgcagcat gttggtccaa caaatacata
     8041 gaatcgtctt tgctcaaaat tatataagta ttaaatacaa ctccaattac agaatatccg
     8101 tataaagaaa acttgcgttt tgaactacac aagtattctt cttcactgtc cttaatacta
     8161 tctcctaagt aataatttcc ttcattttta attccatcaa cttgaaactc ttcttgtata
     8221 ttatcatttg cactatcttc acttagataa ttaatgtttt ttacatcatc ttctacactt
     8281 gcatttagga cttcacttac tgattgaaat tcatttattg gtttttcact tgataaagta
     8341 acaacttcaa tgacttcatc cttaccttta gtaatattac tatttttagg agtagattct
     8401 aataaatcat tatttcttaa tttataatct gtactttttt ctctactgtt aatagcgatt
     8461 ctaggttgtg tttttttatc actataagta gcatattttc caataagatt tgagtgtatt
     8521 aatttaacct ttaaaaaatc tctaagttct atatacactt cttgttcttt ttcaaattta
     8581 atttcaagtt tattggggtg tatatttaca tctatacatg atgggtctac ttctatattt
     8641 aagaagcaca ctgcatgttt acctattgga attatagact tataactttc tgttattgca
     8701 tctatgatta ttttactttt tacaaatctt ttatttatat agatatgttg taagttttta
     8761 tttgaacggt atatattgtt attacctata tatccattca ttttaaagtg attgcactta
     8821 aactctacat ctattatatt ttccgttatt tcttttccat atatacttct aatagtgttt
     8881 acaagctttc catcccctgg agtatttagc atctgtttat tgttatttgt atatttaaat
     8941 tgaatgtttg gattacctat tgcaagttta tttatcaaat cacttatatt tatagtttct
     9001 gcatgagtag attttaaaaa tttctgtctt gctggtgtat taaagaaaat atcttttata
     9061 atgatggtag ttccattagt agaacctatt ggttctttcg atattatttt tccaccttca
     9121 acatatattt tagttcctat tatttcatct ttagttttag ttgtcatttc taattttgat
     9181 acagctgaaa tagatgccaa tgcttctcct ctaaaaccaa gagagtataa atcatacaaa
     9241 tcatcaattt ttttaatttt actagtagca tgtctcaaaa atgatttttc aacttcactt
     9301 gaaggtattc ctattccatt atctgtaatc ttaattagac tttttcctcc atcaattata
     9361 tctatggaaa ttttatttgc acctgcatca atggagtttt caataagttc ttttactaca
     9421 gaagaaggtc tttctacaac ttcacctgct gctatcttat ttatagtcaa atcatctagg
     9481 atgttaatta tatttttcat ttttacacct ctaagatttt atttcttttg cttttctttg
     9541 aagattataa agagagttta tagcatctaa tggagtcata tttaaaatat ccagatttaa
     9601 aatttcttct gatagtatat ctctatttac actatcaaaa gagatttgag ttaaggctac
     9661 ttcttttaca gaatccactt gattaatttt ctctttatca tcattttgct ttattaaagc
     9721 aacttcatca tttaatgctt taaatttaga atttaattta tcataatcct tttttatatg
     9781 tttgtagtct tttactataa gttcatgttc tatttttaga gattcatatt tattcttaaa
     9841 attactttga tttaccttaa ctaattcttc gtccaaatta ctattaatta tattattgtt
     9901 ttctttatct ccatttattg caacactgtt gtaaacatga tttttttcca agtctttaag
     9961 tatatatttt gctctttcta tgacttcgtc tggtaattta gctaatttag caacatatat
    10021 tccataactc ttgtctgctc cctgaggaat tatttttctt aaaaatatta ttccttcccc
    10081 atcttctttg actgctattg aataattttt aacctcttta aactcttctt ctaaatcagt
    10141 aagttcatga taatgtgttg caaataatgt cttacatcta atattttttt gaatatattc
    10201 cactattgac catgctaaac ttataccatc ataggtacta gttccacgac ctatttcatc
    10261 taatataact aaacttctct ctgttgcatt ttttaatata agggatactt cgttcatctc
    10321 taccataaaa gtagattgac cttgagataa atcgtcacta gctccaactc ttgtaaatat
    10381 tctatctaat attggaatat cagcagactc tgctggtaca aatgagccta tatgtgccat
    10441 aagagctatt atagcagttt gtctcatata tgttgattta cctgacatat taggaccagt
    10501 tattatgtta ataatgtttt ctccacgatt taaataagta tcatttggaa cgaaattttc
    10561 ctctcctact atattttcga caacaggatg tctaccattt cttatatcaa gtttattatt
    10621 ttcatttatt gctggtttta catagttatt tatatgtgca actgttgcaa gggacacaaa
    10681 aacatctata ttagctatag tcttagccac tttttgtatt ctatctatgt ttttatatat
    10741 tgtatctctt atctctacaa atatctcgta ttctaaagat ttaatctttt cctctgcatg
    10801 tagtatcttt tcttctattt cctttaattc tggagttata tatctttctg cattactaag
    10861 tgtttgcttt cttatatatg tctcatctag ctttgcctgt ttaaagttag cttttgttat
    10921 ttctatataa tatccaaaaa ctttattaaa gcctattttt aatgatttaa ctcctgtttt
    10981 ttctctttcc ctgttttcta tttcctttac aagaaaggct ccattcttag aaatctctct
    11041 tagttcctta agttcatcac taaaatctga tttaattatg tttccatctt ttatggttat
    11101 cgtaggttct tctagaatag cttcatctat taaattgtat atatcatcta atttatccat
    11161 ttctgaaaca tactctttta ggatttttgc atttgataaa tttatagtat cttttaaatt
    11221 aggtaacttt tctatcgaat tttttaagtg aattaactct tttggtgtta ctctctcaaa
    11281 tgctatcttt ccacatattc tttctatatc gtatatattt ttaagtatat catttaggtc
    11341 ttctcttaat ataaagtcat cttttatttc ttctattaca tctaatctat tttcaatttt
    11401 agatttattt atcaaaggtt cttctacata ttttcttaaa agtctacctc ccattgctgt
    11461 agaagtttta tcaagtacgt gtaataatga accttttttc ttattccctc ttatagtttg
    11521 agtaagttct aaattagttc tagtaaacat atctagaacc atatattctg atgaattgta
    11581 tatatttata ttattaatgt tagatgttat ttgtttttga gtattgtata tataattcag
    11641 aagaattgat aagcttgact ttattagacc tttatcatca aactttagtt tttgtagata
    11701 aacatctgga aaatattctt ttaatatatt tatatccaaa tagttatcac taaaactttc
    11761 atttatgtat atattactta cagtagctat atctctaaga ttttctataa agtctaaatc
    11821 atttataatt atttcagttg gatggatttt tgcaatttct tcaattacct tatcttcatt
    11881 taaacatgtt gcattagttt ctcctgtact tatatcaaca taagttagac ctatattagt
    11941 accatcctta tacaaagata ataaataatt attcttttta ttttcaagta aatttccatc
    12001 taaaactgta ccaggagtga tgactcttat aacctctctt ctaacaattc cttttgctgt
    12061 agatgggtct tccatttgtt cacctattgc tactttatat ccattttcaa ctaattttga
    12121 tatatatgaa ttggctgagt ggaaaggaac accacacata ggagcacgtt cttctaaacc
    12181 acaagctttt cctgtcaatg ctatttcaag agcttttgaa gctactaatg catcttcaaa
    12241 aaacatttca taaaaatctc caagtctaaa aaacagtata cagtctttat atctattttt
    12301 tacttcaaga tactgtttca tcataggagt taatttattc atatctattt gcattatcaa
    12361 acctccttct tttctaatta aatattatat catagtagat gattattatt aacacaatat
    12421 ataaaatttt gacatataac taaaaacaat ccaaaatgta tactcacatt ttggattgct
    12481 aagtttaatg cttattaaat aaaaatattt tttttacaat aaagatatta tttctttttt
    12541 aagtttaatg atattttcaa ggttatcttc atttaacttt ctactatcta taacaaattc
    12601 tttctttact attgatggtt tttttgaaat aacataaatt ctgtttgata gcattatagc
    12661 ttcgtcaata tcatgagtta taagaagtat tgttgaattt acttttttct taacatctaa
    12721 aagccatttt tgcatggatg ttttagttaa tgaatcaagt gccccaaaag gctcatctaa
    12781 aagcattatg tcattagaat ttataaaagt tctcaagaag ttagctctct gtctcattcc
    12841 tccagaaagt tctgaaggat atttatcttc atatccgctt aatccaaaca cctcaaaata
    12901 aggttttacc attgacctag ctttactctt tttttctttt tttaaaatta gtggtagtga
    12961 cacattatct attatagttt tatatggcaa gagtaaatct ttttgataca tataactata
    13021 cttaccattt atattcactt tgccactatc ataatctgta agtttagtta taatattaaa
    13081 aatagtactt tttccacatc cacttggccc taatatagaa acaaactcac cttcataaac
    13141 atcaatactt atatctttta aaacttcaat atctttataa gtttttgata cattttctat
    13201 agatactttt acttttttat tgtggtaaaa attcatttgt gtaagcatca cttgccttca
    13261 tatccttatc tattaattta tattctttta agaatgatgt atagttgttc catacactat
    13321 ctttcatttc tccccatcgt ggtgcatcat ctttgtactt gcttgctaaa tattcttgac
    13381 tctttaaagc tagtttttca tctacttcag gtgcatgttt tactaaaatt tttgctgatt
    13441 cttctggatt ttttattgca tattcataac cttttgttgt agcttttaag aattttttag
    13501 caagctctgg attatcttta agcacagttt cattagaaat tataagtggt gtataataat
    13561 ccaatctctc atctaaatct tttactggta taaagtttaa gtcatatcca attaaatcag
    13621 ctttaactgc atcccaacct tcaaatatcc atgcaaaatc tacagttttc atagctgcaa
    13681 agaaatcatc ttgaccagta ttaactatct ttaacttatt aaaatcagct ccatttttct
    13741 tcataactgc tttaaaaaca gcttcttctg atagagagcc ccaaccacca taagtttttc
    13801 cttcaaaatc tttagctgtt gttatattct tttcttttgg tgatgcaaac ccagaagtat
    13861 tatgttgtat aacagttgct acagctttta ttggaagtgg atcttcacta gtctttgcat
    13921 aagtaacttg ttcttgatag cttataccaa agtcagcttt tcctgttgct actaaagttt
    13981 ctgctccact ttcaggtggt tgtactattt caacatccaa accttcttct ttatagtaac
    14041 ccttatcaag tgctacaaat aatccagtat gatttgtatt tggtgtccaa tcaagcacca
    14101 tagttacttt tttagaagaa gtatctttat cattgttttt gtctttattg ttatttgaag
    14161 aacatccagt tattgttgcc aatgtaaaaa cacttactaa acctaatatt cctaactttt
    14221 taaaattcat aattgcctcc ctcaaatacc tttaatctat taatctatgt cttataacaa
    14281 ctttttttac agcctgtaca attcctacaa acattaaact aaatataact actaatatag
    14341 tagatgcaaa tactttatca agtgcatatg cactttttga cctaagcata taaacaccaa
    14401 gtcctttcgt tccacctagc cattctgcaa cagttgctgc cattacagca tatgttgcag
    14461 atattttaag tccagaaaaa aatttatcca ttgccatagg aaatttaagg tgaatgaatg
    14521 tattaacttt atttgagtcc atagtcttaa ataaatttaa ataatcttta tcaatattct
    14581 caatgccatc tacaaaactt attaatatag gaaaaaaaca agtaagcatt accatcaata
    14641 cttttggcat tgttccaaat ccaaaccata tcataaagag tggtgctata attattgtag
    14701 gtatcatctg tgtaaccaac ataataggat aaagacattt ttttattatt gatacaaaat
    14761 ccatcaatat tccaattacc aatgatatta taattgcgac tataaaacca agtattgctt
    14821 ctcctatagt aaccacagca tgcatggata aattttgata atctttaaaa aacacattaa
    14881 ttatatctac tggtgtgggt agtatatact gaggaacttc taaaactcct accatagatt
    14941 gccaaagaat aagtataaac aaaaatgttg ctattggata tatactatct ttgactttat
    15001 ctatgatttc taagtttttc atctatagtc agacctccag ctttttcatc tactctagct
    15061 actgttatga ctctttcaca tccaagatta aaagggattt tatgtacctt ttttaataat
    15121 tcaaaaactt catcaaaatc accttcaaca gtagtgctca ttgctcctat ttcacattta
    15181 agaccacttt tttcaatttc agcaatacaa gcatctacta ctttatacat ctcttcttca
    15241 cctttgtaag gtcttagtgg cattactgct atgtctgcga ttaccattta aatcactcct
    15301 ttaaaaataa ttaataccta ctaggtatat tttgtttttt attaaaaaat tttattacta
    15361 aaaagatatt atatttaaat aaaagaaaag tcttatccaa aataggataa gacttatttt
    15421 tatcttaact ggtctcccta cactggcatt atccaaacag gttcaaaggg tcgagataaa
    15481 atctcctctc agcacattag ctccccgaaa tatattatat cttttctact tgattatact
    15541 acacttatca tattttaaca aattttttat ttatattaga tattttatcc gttcaaaaat
    15601 atctaatata aattgtacat tattacacca aataaattat cttaaaatgg tattttatca
    15661 acatcttcca aaactagatt aactgaattt tctagttttt attttgctat ttcaagcata
    15721 tttggatgta tatcaattcc atacaaatat tgtgctaaat ttctgttttc actcatctaa
    15781 tttgcaatcg ttacagatta cagattagaa acagttttta gactctactt atctttatct
    15841 acaaaaaatc tttcaataaa ttcattaata atattcagta atctaaaaat aaacctctat
    15901 ctggattttt aagatacaaa ccttttagga agtatctttt gtgataatat aaaaattact
    15961 cgtcattatc atcaaaatcc tcagcaacca acattttata taagaatcaa actctataat
    16021 ataattattt tatccaattt atttttgaga aataaaatat ttttttaatt tttaaatttt
    16081 ttaatatgca ccttga
//
LOCUS       DAGSWI010000094        15938 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.094, whole genome shotgun sequence.
ACCESSION   DAGSWI010000094 DAGSWI010000000
VERSION     DAGSWI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.094"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            185..562
                     /locus_tag="MJF80_002682"
     CDS             185..562
                     /locus_tag="MJF80_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0281333.1"
                     /translation="MKEEILVEKLLRAELIVMEYLWKKDSLVTKKEIVKEMRQVYRWH
                     KSTIKILLKRLVDKGYLARDIIKFQSHYKIIIDNKEYYAFKKKVLKSSKSRKIMRSLT
                     TTHKSISKEKLDALEEYYRNLEE"
     gene            672..1055
                     /locus_tag="MJF80_002683"
     CDS             672..1055
                     /locus_tag="MJF80_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0281334.1"
                     /translation="MKISKLPEAELKVMRYIWEANDVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNAMVKEKKYLRFETKEFLKNIHKNSISSLISALH
                     SKDDEVDEDKLIELEEHFKNLKEKE"
     gene            1240..1824
                     /locus_tag="MJF80_002684"
                     /pseudo
     CDS             1240..1824
                     /locus_tag="MJF80_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            1850..2263
                     /locus_tag="MJF80_002685"
     CDS             1850..2263
                     /locus_tag="MJF80_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0281335.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            2267..3010
                     /locus_tag="MJF80_002686"
     CDS             2267..3010
                     /locus_tag="MJF80_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBZ0281336.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            3010..3480
                     /locus_tag="MJF80_002687"
     CDS             3010..3480
                     /locus_tag="MJF80_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0281337.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            3497..4255
                     /locus_tag="MJF80_002688"
     CDS             3497..4255
                     /locus_tag="MJF80_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBZ0281338.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     YTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            4239..5081
                     /locus_tag="MJF80_002689"
     CDS             4239..5081
                     /locus_tag="MJF80_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBZ0281339.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(5241..6146)
                     /locus_tag="MJF80_002690"
     CDS             complement(5241..6146)
                     /locus_tag="MJF80_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBZ0281340.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            6508..7242
                     /locus_tag="MJF80_002691"
     CDS             6508..7242
                     /locus_tag="MJF80_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281341.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            7453..8634
                     /locus_tag="MJF80_002692"
     CDS             7453..8634
                     /locus_tag="MJF80_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBZ0281342.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            8618..9190
                     /gene="mobB"
                     /locus_tag="MJF80_002693"
     CDS             8618..9190
                     /gene="mobB"
                     /locus_tag="MJF80_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBZ0281343.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            9309..9584
                     /locus_tag="MJF80_002694"
     CDS             9309..9584
                     /locus_tag="MJF80_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0281344.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            9839..11539
                     /locus_tag="MJF80_002695"
     CDS             9839..11539
                     /locus_tag="MJF80_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281345.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            11902..13089
                     /locus_tag="MJF80_002696"
     CDS             11902..13089
                     /locus_tag="MJF80_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281346.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            13149..14312
                     /locus_tag="MJF80_002697"
     CDS             13149..14312
                     /locus_tag="MJF80_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0281347.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            14463..15266
                     /gene="zupT"
                     /locus_tag="MJF80_002698"
     CDS             14463..15266
                     /gene="zupT"
                     /locus_tag="MJF80_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBZ0281348.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            15362..15922
                     /locus_tag="MJF80_002699"
     CDS             15362..15922
                     /locus_tag="MJF80_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBZ0281349.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKTNKVKSVLFMNMFVFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYVVQLYRRLMYYLRLKKFK"
BASE COUNT         6208 a         1655 c         2868 g         5207 t
ORIGIN      
        1 tagaattagt tattattcaa caggatagtt tgaaaattga ataattctat agatatgaaa
       61 gttatttttc ttctaagaaa acaaactaaa acagtagaat atttggtaaa aattacctat
      121 agtttttgtc ttaaaaagct cttattatta acttgaacta gaacaatcga aggagtgaca
      181 taagttgaaa gaagaaatat tagtagaaaa attattaaga gcagaattaa tagtaatgga
      241 gtatttatgg aaaaaagatt ctctagtgac taaaaaagaa attgtaaaag aaatgagaca
      301 ggtttataga tggcataaaa gtacaataaa aatactacta aaaaggctag ttgacaaagg
      361 ttatttggcc agagatatta taaagtttca atctcattat aaaataataa tagataacaa
      421 agaatattat gcttttaaga aaaaagtgtt aaaatctagc aaaagcagaa aaataatgcg
      481 ttctcttact actacacata agtctataag taaagaaaaa ctagacgcat tagaagaata
      541 ttatagaaat ttagaagaat aatttcgaga tggaaaaaat tggatggtaa tatttagtgt
      601 gattctcttg tttggaaaac tttattgaga tagaatttaa catatacata attcgataaa
      661 ggggtgtgac tatgaaaatt agtaaattac cagaagcaga attaaaagtt atgagatata
      721 tttgggaagc taacgatgta ttaacgtcta gggaattagt tgccgcaatg gaagaaaaat
      781 acgaatggaa tgaatcaact acattcacag ttttaaaaag gttagagcga agggagtttt
      841 taagtacaga gaaaataggt aaacttacac actataatgc aatggtaaaa gagaaaaaat
      901 atctaagatt tgaaacaaag gaatttttga agaacataca taagaattca atttcaagtt
      961 taatatcagc gttacatagt aaagatgatg aagtggatga agataaatta attgaactcg
     1021 aagaacattt taaaaattta aaagagaaag aataaataaa gattatttta ggtggcaaat
     1081 tatttttgcc accttttttg ccaccatgac agtttaataa aataaaatac aaataaagaa
     1141 atataaataa aatagaataa agaaattgtt ttagataggt tgtaaataaa tgtactcata
     1201 aaaacataaa taaatataaa taaacataaa taaaatataa gaatttctgt gagaaatgca
     1261 cttaatgaac ttgtaaaaga agatatatta tgtaaaaaac gtggtaaagg gacttatgta
     1321 acaataccag agagaataga agcaacatgt gcaggaaata gttttactaa ttcttgtcat
     1381 cgtattaatg caaaaccaag taccaaaata atctctgttt taataaaaaa agcagataag
     1441 caagtagctg aagctttaag tattgaacta gaagagaagg taatatgtat taaaagactt
     1501 agattaatag atgaagtacc tgttattttt gaagttgact attttagaat agattacatg
     1561 tttttattaa aagaagaatt agaagataaa tctttaatgg aggttatatc taataatata
     1621 agcacattac ctaaacgagt agaaaatata tttgaagtaa aacattcaaa caaagaatat
     1681 tctgaccatc ttaaatgtgc aagtaatatg ccactattga aagtaagaca atcggtgtat
     1741 acagaaaata atgatgtctt atattacaat gagcaattta taagaagtga taagtacaaa
     1801 tatgcagtat cagcagaaat ataatctata atttagtagg aggatgagta tgaaaaagat
     1861 aatattagca tctcatggag acttttcaaa agggctttta aatgcagtaa aaatgatagt
     1921 aggagattta gcagattgtg tcagttcgta tggtttgtac cctggacaaa gtgcttcaga
     1981 ttttgcttta gaattagaaa aaacaatatc agaagataaa gaatgtgaat atataatttt
     2041 atctgactta tatggagcaa gtgtatgtac agcaatgtta agattaacta atctacataa
     2101 tgttaggtta ttttctggaa tgaatttgaa tatggtatta gaacttctta caaggttttc
     2161 tgatgaatta acagttgaag atatagacca attagtggaa gaatctagaa gaggaataca
     2221 aagcgtgaca ttacaaatta aagaagaaga agaggtattt tagtttatga aaaaaaaggg
     2281 aatattatca gtatttaaaa gtaaaaaacc aattatagca atgatacact taaaaggaga
     2341 tactccagaa gatatctttg aaagagctaa aaaagagatt acaatctttg aagaaaatgg
     2401 tgtagatgga ataatgttag aaaattacta tggtaattat tatgatttag aaagaatact
     2461 tgaatatgta tcaaaagcta atttatcaat accatatgga gtaaactgtt taaatgttga
     2521 tactatggga tttgaactgg caacaaaata taatgctagt tatatacagg ttgattctgt
     2581 agtaggtcat gttaaaccta gagatgaagc tactttagaa gagtttttta aattacaacg
     2641 ttcaaaatgc ccagcgtatt taattggagg cgtacgtttt aaatatcagc cagtattatc
     2701 agaaaatgat gttgaagaag atttaaaaat aggaatgaca agatgtgatg ctatagcagt
     2761 tacagaaaat gctacaggtc aagaaacatc aatggaaaaa atagaactgt ttcgtaaaaa
     2821 tttaggagac ttcccattgg ttatagctgc tggagttaca ttagaaaatg caaaaaaaca
     2881 gcttaaatta ggagatatgg caataatagg aagttacttt aaagataatt acaaagattt
     2941 tggggatgtt agtgtagaac acgtaaaaac attcatggat gaaatcaaaa aaattaggga
     3001 ggaattataa tgattaaatt agtaagagta gaccacagac ttatacatgg acaagtagca
     3061 tttacatgga caaagttttt aagcacagac tgtatattga tagcaagtga tgacttatta
     3121 aaagatgaat tgagaatggc aggacttaaa atggctaaac catctaatgt taagttagta
     3181 atgaaaagca tagcagattc tataaaagca cttaactcag gtgttactga taaatacaac
     3241 ttattgatac tttgtgaatc tgtagaagat gtttatagac ttgctaaaga ggtaaaagct
     3301 attaaatcta taaaccttgg tggaacaaaa tcagatgata atcgtgaaaa tatatctaaa
     3361 gcagtgcatg tatcaaaaga tgatataaag atgattaaag agttagattc agaaggcgta
     3421 aatgtatttg tacaattagt tcctgatgat gatgctacaa atgtaatgaa attaatataa
     3481 ttttaggggg atgaaaatgg aatttacaca agttattttg ataactctaa ttgcattctt
     3541 tgcatatatg catagttttg taggttctac aatgcataat agacctatag ttgttgcacc
     3601 acttgttggt ttagcactag gtaatcttta tacaggtata gtaataggtt caacattaga
     3661 attagtattt atgggtgcat ttcctgttgg ggcaagtaat cctcctgatt ttgtgtctgg
     3721 ttctattatt gccacagcgt ttgtaatatt aacgggtcaa gatgtatcag cagcagttgt
     3781 acttgcagtt cctatagcaa ctctagtatt gcttatagat aacttcttaa tgacagtagt
     3841 acttacatgg ggagctcata ttgctgaccg ttatgcagaa gaggggaata ttgaaggcgt
     3901 agaacgtgtg caattattat ttggtatagg aaataaatta atattagcaa taatagtagg
     3961 tataggattt tcattaggtg ttccagtaat agaaaaaata ctttcattta taccatcata
     4021 tgtaactcat ggaatggatg ttgcagcagg cgtgattcct gcaataggtt ttgccatgtt
     4081 agctagaatg atgcttaata agaaaacagt agcattttta ttattaggat ttatattggt
     4141 tgcttaccta aacataacag taacaggagt tgcattgttt ggattagcaa tagctttaat
     4201 atatgttaac tttgtaggag agaaggaggt aatagtagat gacaacgagt tctaagaaat
     4261 tagagacaat ttcaccagat agtaaaataa cgaggaaaga cttttggaaa tgttttagaa
     4321 ggtcattgac attagattca tcttggaact acgaacgtat gcaaaatata gcatatgcat
     4381 acatgatggc accaataatc cgtagattat ataaagatga taaagaaaag aaatctaagg
     4441 ctttaaaaag acatttagaa tttatgtcag ttactcctca tataagtact cttttggtag
     4501 gtatatctgg agctatggaa gaagaaaatg caaagaataa agaatttgat gctaatagta
     4561 taaatgctgt taaatcaagt ttaatgggtc cagtttctgg gataggagat tcatttttct
     4621 ggggaacatt aaagcttata gcagctgggg ttggtatagc tttagcttct cagggtaaca
     4681 taatgggacc gattttattc cttttaataa taaatgttcc tcattttatt atacgctata
     4741 tatgtctaga taaaggattt aagtatggaa ctcaattctt taaagatgtg agtggttcaa
     4801 gtatagtttc aaaagttatg gaagcagctt caatgttagg gcttatggta attggtggta
     4861 tgactgcatc taatgtaatg ttaaaactta gtgttaatgt aggtagtgga gaatgggcag
     4921 agcctattca aacttactta gaccaaataa tgccatgtat gcttccagct atgatattcg
     4981 gtattatgta ttggttatta ggaaaaaagg taaagacaac tacgatatta atttcagtta
     5041 tgattatttg tatagtattg gctgctattg gagtagtata aataaaaaaa tcccaagttc
     5101 atttgaacga gggatttttt gactaaatta aataaaaaag aaagaaggtt cataataatt
     5161 tataagcatt tatgtgatag ttataattag cttattaatt agttataaac tcaaaattaa
     5221 gcactacttt attaaagtta ttatattata atttcgaaat actcacataa tctgtttaga
     5281 aatttatctt ctaatgggga aaaagttttc tttttagagt aaatgaagaa aaactttcta
     5341 gtaaaatcaa catcctttat tttataacat tttattttac ctgcattgag ataatcgata
     5401 gctgaaatgt acgatataaa ggacactcca agacctaatc taaccatttc cttaatagcc
     5461 tcatttgatt ctacatgagc gattacattt aatttactta ctggaaagtt atttttacta
     5521 agtgtgttta gtattaagtt tcttgtacct gacccttctt ttctcataat aaaatttaga
     5581 taagccaatt ctcctatatc gatacaatta tttttattat caattttaaa atctgaagga
     5641 gtgataagaa caagttcatc atctaataag tctaggtact ctatttgagg attattgatt
     5701 ttagaaccaa ctaatccaaa gcttattcgt tcatttaaaa tttctgatat agcatattga
     5761 gaatcataat ggcttatgga aaattttaca tctggataac tcatagaaaa actcttcata
     5821 aaatcaggta aaatataagt ttcaggtatg gaactacatg ctatatctat gataccttct
     5881 atttttcctg aatattcttt tatatcataa attgcacgtt tgcaattatt caatatatat
     5941 atagcatgtt cataaagaat ttcaccagat tttgtgagtg taataacttt gttgcttcta
     6001 tttataagaa cagtttctag ctctctctct aaattctgta tgtgtgcgct tacagtaggt
     6061 tgagttaaaa aaagttctct tgcagccttt gaaaaactct gatgttttgc aacagcaaca
     6121 aatacttcaa gttgtttaaa atccattatt taatcctcct taaattatct aaaactatta
     6181 taacatattt gataaattaa tagtcaaagg tgatttttaa aatagaaaac ctttaaatat
     6241 tcatggagtt tttgattgtt taggaataat ttggtataat gttgtaaggt acttacaata
     6301 aaaatatcgt aaattgaatt ttcaaaattc aactttaaag tgttttttat tacagaaaaa
     6361 ttacaattag cttcttaatc ttattaaatt caaataaata ttatataatt attttataat
     6421 ataataaata taattattga aaaaagagat aactgtaaat tgagatatag ataaatacta
     6481 taaaatttaa gaaaggagga ttgatttatg aagttaaaaa ggtcattagt ttgtgtaact
     6541 attttaggta ttatacttgt tggatgtcat aaagaaaata ctaaagagaa aaatcaagta
     6601 gcaagtaaag caacacagca aaaaactatg acaaaagttc aaaatgatgt taatgaaatt
     6661 atgaataaag attataagta cattataaag aatatgggta taccttataa tacattttat
     6721 tatataaaac ctaaagtttt aaaagaatca aatacaatgc aagatataaa tacatcaagt
     6781 tatatgacat tagtttatcc aaaatatact ggaaatgatg aactagatgg aagtgcttta
     6841 tatgttgata ttaacgaaaa taaagttgtc aatgttgaaa ccaattcttt ctcatcacaa
     6901 ggtatatcag tcattgatgc tgaatctagt atagtgattg aaaaaagtga ccatgaaaaa
     6961 tcggctgtat cactagaaaa ttttagacat attgacctgg gagagtatgt tggtgttgaa
     7021 gattccagaa taaatgaaat tgtaggagat gcgaattatg atttaacagc atataatcat
     7081 gaaggtagta aagtagttaa gagttataga ttgaaagaag ataacaaaat attaaaaaaa
     7141 gaggtactta caataagtat tgtggataac aaaataaaat ctataaaaac tattgaaagt
     7201 gataaaattg taaaaataat aaaaggaact ttattagaat aaaatattct aaagcatatg
     7261 atgctgtttc aagatagaag gaaatttcta gctgggaaca gtttttttat tagatttata
     7321 aaatttgaac tgcacttata gataactatt agttataatt aaatataaga taaaagtatt
     7381 tccaactttt ataatctata atatattaca tagaaatatc ttaaataaaa aatatattgg
     7441 ggagtattta aaatgataga attagaagat gccataaaaa ttattgaaaa aaatgtttta
     7501 ccaataaaca gggtcaagat agtaaatctt atagatgcag taaataaggt agttgcagaa
     7561 gatatatatt caacaattga tagtcctcca tttgataggt caccctatga tggttatgca
     7621 tataatgcaa attctgaaaa tagaaattta aaggttatag ggactttgta tgcaggagat
     7681 gtatttaata gggtattaaa taaaaatgaa gcagtgaaga taatgactgg tggaaagata
     7741 ccacagggtg caaattgtgt aataaaaaaa gaagatgtaa cagttatagg tgatatgata
     7801 actttaaata taaaattaaa ggaatacgat aattacatat ttaagggtga ggatataaaa
     7861 aaggggcaat tgattattaa taaaaatcag aatataggat atgatggtat tggagtatta
     7921 gcaagtcttg gaatatccaa agtaactgtt tatgatgatt taaaagttgg aattttgaat
     7981 acaggtacag aactacaaga tataaatgag actttagaga atggtaaaat atacgatagt
     8041 aatagatata ccttatattc aagattatta aaacttggaa tacaagcaga tacaatatgc
     8101 aattgttctg atgatatatt ggagttagaa aaaaaggtaa agaatatgtt acagagtgta
     8161 gattttttaa ttaccacagg tggtgtttct gttggagata aagatttaat tccagatgta
     8221 ttcaaaaaaa taggggcaac agaattattt tggaagataa acattaaacc tggtggagct
     8281 tgttatgtag gtacattggg agaaaagcta ttatttggtc tttctggtaa tccacatgca
     8341 gcaatagtgg tatttgataa tgtgatagtt cctgttattg agtatttaac atataagaag
     8401 ggaacacatc atataaaagc tatatttaaa ggagaattta ataaactttc aaataaagat
     8461 agatttgtaa gtggaaaact atatacaagt gaaggtaaat tgtatgtaga gctaaatgat
     8521 aagaaagatg caaaagctag actatctgca actctaagaa gtaattgtat gattaagatt
     8581 aaaaagaatg agacaataaa agaaaatcaa ttagtggatg ttgttattag atagttttat
     8641 gaaaggacac aattttatgt ataatatatt gtcagttgtc tcatattcag gagtaggtaa
     8701 aactacgttg attgaaggca taataaaaga attaaataaa aaagggtatc gtgttggagt
     8761 tattaaacat acctgtcatg attttgatat ggatgaggaa ggaaaagata cttataaaca
     8821 tagaaaatct ggggcgagaa aagtctgtat aatatcagat aaacgagtag catatattga
     8881 agaactaaaa gagaagaatc cattatataa aatgattcaa ttatatgaag atatggactt
     8941 gattatcata gaaggatata aaaattatag atttaaaaga ttggaagtaa ctagaaaagg
     9001 taagtatgag gatattttat ctaataagtc tgatttgata ggtattgtca gtgatataga
     9061 atataaattg aatattgaac agtttaattt aaatgattat aaaaatatat caaagtacat
     9121 agagtcttgt ataaaaaatg aaattttaag tataagcaat gttgaaataa aaaacatatt
     9181 aaaagaataa ttttaagatg ttcttcttat ataaaatgat ataattattt atataaataa
     9241 agatataaga aatttatcaa ttttattttg agttataaaa tattaaagaa attgtaggag
     9301 gacaatatat gaaaaaaata ttagtagcct gtggagcagg aatcgcaaca tcaacaatag
     9361 tttgtgacag agtagaaaga ttggttaaag aaaataatat acaagcagaa gttgagcaat
     9421 gtaaaattgc tgaatgttca acaaaacaag aaggtgctga tttaatcgta tctactacta
     9481 tactaccaac aacttatgat atacctacaa taaaggcaac aggatatata acaggtataa
     9541 atacaacagc attagataaa aagatattaa atgcattgaa gtagttttgt atttataaaa
     9601 aaaatttagt attaaatata gagttgtatc tttataaaat ttaaaatata gagatatagc
     9661 tctttttatg ttgtttcaag tatacaacta tatgatataa atcttcaatt ttatgaaaaa
     9721 gattgttatt actttgtaag tttataaaag atttaaagtt attgttatgt tatactaatt
     9781 tatgggtatt ttttagaagt actaatagaa gtactaataa aggagggatt taatacttat
     9841 gataaaacaa tttgtcaaat attataagcc atataagaaa atttttacac tagaccttat
     9901 agcagctttt ctattttcct tatgtgattt agtttatcct atgataacta gaaatataat
     9961 ggatgacgtt gttccaaata aaaacttaag aatgttagta gtttttgcag tagcacttat
    10021 acttatattt atagcaaaag cagggcttaa ctattttatg cagtattggg gacatgttat
    10081 tggtgtagat atgcaagctg atatgagaaa tgaggtattt acacacttac aaagacttcc
    10141 aaatacgtat tttgataata acaaatctgg agtaactatg tctagaatag taaatgattt
    10201 aatggatata acagaacttg cacatcatgg gcctgaagat ttattcattt ctatagtaat
    10261 gttggttggt tcattcttta tacttataga tataaatatt cctcttacgc ttataatatt
    10321 tgctatatta ccgtttataa tttggtttgc aatagctaaa aaagataaga tgaatattgc
    10381 ttttatgaag agtagagtta ctataggtga tgtaaatgct actttagaaa atagtatagc
    10441 aggtatgaag gtaactaagt ctttttgtac tgagaaagaa gagttaaata agtttgtaag
    10501 aagcaataaa ttatttagaa gagcaagaca agattcttat aaagtaatgg cagaatatta
    10561 ctctggtatg aatttataca tggatatctt agagtgggta gttgtaatag ctggtggtta
    10621 ttttacttat ataggaaaga ttactttggg tgattttgct gcttatatac tatatgtaaa
    10681 aatgtttata caacctatga aaaaattaat aaactttaca gagcaatatc aaaatggtat
    10741 gacaggtttt aaaagattca tagagataat ggaacaagac catcaaaaag aagctaaaaa
    10801 tcctattgaa cttgaaaatg taaaaggtga cattgagata gaaaatatat ctttcacata
    10861 tgaagataag actcaagttt tggataattt aagtttatct ataaaagctg gtaagactat
    10921 agcattagtt ggaccttctg gaggaggaaa gactacatta tgtaatctat tgccaagatt
    10981 ttatgaattt gataagggtg atataaaaat tgatggaaag agtataaaag atgttagttt
    11041 aaaatcattg agaaaaaata taggtatagt tcaacaagat gtatttttat ttacaggaac
    11101 tataagagat aatattcttt gtgggaaccc taatgctaca gatgaagaaa tgatagctgc
    11161 tgctaaaaaa gctagaatac atgactttgt agagactttg ccagatggat atgatactta
    11221 tataggagaa agaggcgtaa aattatcagg tgggcaaaaa caaagaattt caatttcaag
    11281 aatcttttta aagaatcctc caataataat attggatgag gctacatctg ctttagacaa
    11341 tgtaacagaa agagaaattc aagagtcttt agaggaatta agtaaagata gaacaaattt
    11401 agttgttgca catagattga ctactataaa aaatgctgat gaaatcatag tattgacgga
    11461 taaaggaatt gaagaaagag gtactcatga agagctagta aacaaaaatg gagtatatag
    11521 taggttacac aataactagt atacaaattt tacaccatag ctaacataca cattaaactt
    11581 acaaacatag gatatattaa gattcataaa gacaattcat tttatattta taagatgttt
    11641 tatgaataaa atgtatccta tgttttattt tttgcaaatt acatttaatt ctctttgtaa
    11701 gaatagagtt aaatagccaa tggtcgggat aacgttttta caaaaagaat aatttagaaa
    11761 actaacttta atttatggaa tttatcgaat tttatgaaaa aaatcgaatt ttgacttgaa
    11821 atgtttttca aaagtagtaa aataatggat aactatacat acattttata aaaatatatt
    11881 tattatgagg ggggattttg tatggaggca ataacagctt tttgtctagt tttagtatca
    11941 ctgacgatag gagatatagt ttcagcaaaa actaaagctt ttgttccgtc ggtttttgta
    12001 tcagcaatta tatttatagt aggattttgg acttttttcc cagaaaatat tgtggactta
    12061 gccgctttag gaacaccatt agctcaatta ggtatgcttt tgttaataac tcatatggga
    12121 actatgatga gtattaagga attagcaggt caatggaaaa caatagtaat agccttggca
    12181 ggaatagtag gaatatgtgt aggtgctttg gcattaggga cagtagtatt tggatgggat
    12241 acagcagtaa tagctacacc tcctcttact ggagggctag ttgcatctat aatgatggca
    12301 gatgctgcta cagcaaaagg tcttgcaagt ttatcagtac ttgcaatttt gatgtatgtt
    12361 gcacaaggat ttgcgggtta tcctattaca gcattaatgc ttaaaaaaga aggaaaaaga
    12421 cttttatcag actttagaag tggaaaagta actgtaaata cagatgaaga aaaagttaag
    12481 gatttacctg aacaaaaaag cagatttaag ataataccag acttaccaga aaaatatgat
    12541 acgacatata tgataatact tagattagga tttgtagcat ggttagcagt acaatttaca
    12601 aatcttacaa atgaagttgt atcaagatat gttatatgtt taatatttgg tgtaatagca
    12661 tcagaacttg gaatagttga tagaaaacca cttaataaat ctggttcatt tggatggtta
    12721 atgacatcat tgatggcttt tatatttgca gggttagcaa aagctacacc agcaatgctt
    12781 gttgaaatgg cagttcctct tgtaggaatc ataatatttg gtgtaatagg tatgggaata
    12841 ttctctatta tagtaggtaa gcttttaggt gaaagtaaag aaatggcttt ttcagtttcg
    12901 cttacatcac tttatggttt tccaccaaac tatatattaa ctgaagaggc agtaaaggct
    12961 ttggctgaaa caccagaaga aaaagaattt ttaatggata agatgcttcc aaagatgctt
    13021 gttggaggat ttactacagt tacaatagct tctgtagtaa tagcaggtat ctttgtaaat
    13081 ctaatatagt atttaagtga taaaaagtat tttaaataag aagaatgtgt actacttgga
    13141 ggtatggaat ggatattaaa gaaataacga agagttataa agactatgta attaagctta
    13201 gaagggaatt tcatgaaaat cctgagaaaa gtatggaaga agttagaaca tcaaagagag
    13261 ttaaagaaga acttgataaa atagggatac catatgtatc agctggaggt acaggtgtaa
    13321 ttgcaactat aaaaggagct aatccaggaa agactgtagc acttaggggt gatatggatg
    13381 ctctacaagt tgttgaatgc actgatgtag aatacaaatc taaaaatgaa ggacttatgc
    13441 atgcttgtgg tcatgatggt cacacatcaa tgcttctagg ggcagcaaaa gtgttaaatg
    13501 atattaagga tagtatcaat ggaactgtaa aattattttt tcagccagga gaagaagttg
    13561 gtaaaggtgc aagagctatg attcaagatg gtgctatgga aggtgtagac agtgtgtttg
    13621 gaatacatct ttggactgat gtagaatctg ggaccatatc ggtagaagaa ggaccaagaa
    13681 tggcttctgc tgatttcttt aaaattactg taaaaggtag aggtggtcat ggttcacttc
    13741 ctcatcaagg tgttgatgca gtgcttgcaa gttcagcaat agttatgaat ctacaatcta
    13801 tggttagtag agaagtaagt cctctggaac cattagttgt gagtgttgga gttttaaatt
    13861 ctggaactag atttaatgta attgcgagtg aagctatctt agaaggtaca ataaggctat
    13921 ttaatccaga gcttagaaag caaattcctg gaattttaga acgtatagca aaatctacag
    13981 cagaagcata tagagcagat gctgaattag agtatgggta cttaacacca gcagttataa
    14041 atgacaaaga atgttcaaag atagctacag atgcagcaat taagctattt ggagaagact
    14101 gtataacact atttgaaaaa gtaacaggag cagaagattt agctgagttt atgaatatag
    14161 cacctggtgc actagcattt gtaggagcta gaaatgagtc aaaaggtgct tgttatcctc
    14221 atcatcatgg atgctttaat atagatgaag atgctctaga aattggaact gctttatatg
    14281 tgcaatatgc agtggatttc ttaaataaat aattttaata tactataaag aaaaagcaga
    14341 gactaagttt atatctctgc ttttttattg tgtatatttt ttataaatat tgactatata
    14401 aaatatatat gatagcataa agaaaatgat aatcaatatc aattaaaaag aaggtgattt
    14461 atttgaataa tgtaatattt gcatttttga taacattatt ggctggttta tctactggta
    14521 taggaagttg tatagcattt tttgctaaaa aaacaaatac taagtttctt tctataagct
    14581 taggattttc tgctggtgtt atgatttatg tttctatgat agaaattttt cctaaagcac
    14641 aagatgcttt gacaaaatct atgggagaga aattaggaag ttggtcaact gtaatagctt
    14701 tttttatagg tatggccatc attgctgtta ttgataagct aattccacaa gaagaaaatc
    14761 cacatgaaat aaagaaaatg gagaatatag aagaaaagaa tatcaaaaaa aataagtctc
    14821 ttttgagaac aggtatattt acagcaatgg ctatagcaat tcacaatttt cctgaaggat
    14881 tagctacatt tatatcagct cttgatgatg ttacaatagc tattccgata gcaattgcaa
    14941 ttgcaataca caatatacca gaaggtatat ctgtatctgt accagtttat tatgccacag
    15001 gagataaaaa gaaagctttt tattattcct ttttatctgg tatgtctgag cctttaggag
    15061 cgattattgg atatgtactt cttagaaatt ttttaaatga tataactctt ggaattgtat
    15121 ttgcaattgt aggaggaatt atggtattca tatcactaga tgaattattg ccatcagcta
    15181 gagagtacgg ggaacatcat ttatctattt atggattgat tgcaggaatg ggtgttatgg
    15241 ctattagttt attactattt aaatagcttt ttaaatatat gattgtaacc taaaattagt
    15301 atcaaaatga tataattatt taaatagatt tttaagctat ttaaaaatat cattttggag
    15361 aatgagtgct atggaaaaga aaataaaatt caaatttgac tggtatagtt cacctgataa
    15421 gatagagaaa tggttagaag atattgaagc taaaggatat aatttatata agataaataa
    15481 atttggagga atattttatt ttcataaggg aagtccaaga aaaataaaat acactgttga
    15541 ttactactat gatgtagaga ataattattt tgatgaacat aaatcaaatg gctggaatct
    15601 tgtatttagg agtattggta gttttatggg tacatctgca tggtatatat ggtcgaaaga
    15661 atacaaagac atacctccta aattatatag tgattctgat aaaacaaata aagtaaagtc
    15721 agttttattt atgaatatgt ttgtattctt tatgttagta gtaatattta tatatttatt
    15781 gaatattgaa ataagaaatt tatatagtgg ttatggagca tctgtaactg ctattgtact
    15841 acagattata ttgttgattt tatatgtagt acaattatat aggagattaa tgtattattt
    15901 aaggcttaaa aagtttaaat aaataaaata aggtgtcc
//
LOCUS       DAGSWI010000095        15712 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.095, whole genome shotgun sequence.
ACCESSION   DAGSWI010000095 DAGSWI010000000
VERSION     DAGSWI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15712
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.095"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(136..753)
                     /locus_tag="MJF80_002700"
     CDS             complement(136..753)
                     /locus_tag="MJF80_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBZ0281350.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(880..1902)
                     /gene="argF"
                     /locus_tag="MJF80_002701"
     CDS             complement(880..1902)
                     /gene="argF"
                     /locus_tag="MJF80_002701"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBZ0281351.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            complement(2011..3198)
                     /locus_tag="MJF80_002702"
     CDS             complement(2011..3198)
                     /locus_tag="MJF80_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBZ0281352.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVILKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            complement(3228..4088)
                     /gene="argB"
                     /locus_tag="MJF80_002703"
     CDS             complement(3228..4088)
                     /gene="argB"
                     /locus_tag="MJF80_002703"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBZ0281353.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKETE"
     gene            complement(4103..5320)
                     /gene="argJ"
                     /locus_tag="MJF80_002704"
     CDS             complement(4103..5320)
                     /gene="argJ"
                     /locus_tag="MJF80_002704"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBZ0281354.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            complement(5361..6395)
                     /locus_tag="MJF80_002705"
     CDS             complement(5361..6395)
                     /locus_tag="MJF80_002705"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBZ0281355.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     misc_binding    complement(6649..6862)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7165..8256)
                     /gene="ytvI"
                     /locus_tag="MJF80_002706"
     CDS             complement(7165..8256)
                     /gene="ytvI"
                     /locus_tag="MJF80_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBZ0281356.1"
                     /translation="MNEQDHTSYQNKQRFLINFCYLVVILGLLMLLGKYAFSVLLPFV
                     FAFLIASLLNKPVLYISEKLLIKRGIVATISVFLFFLIAGIIVSVIGTYLIYGIKEIF
                     HFLPTMFEELVIPLTETVIKEVELFSGSFNFSFIELIEANMPVLMNEMSVFIVSLSNN
                     IVSGITDIVSSVPLLFMKTIITIVASIFIAIDYPTIKRFIVLQIPKNKSYLLAEAKAF
                     TIDTIIKCGFSYLLIFAITFSELYVGFAIINISYAGIIALFIALLDILPVLGTGSILI
                     PWCIISLFLGDYSMALGIALLYIVITIIRNIIEPKLVGKHMELHPVLALASMLTGLHF
                     FGFIGLFGIPLLIAFLKKLNDKEIIHILH"
     gene            complement(8271..9662)
                     /locus_tag="MJF80_002707"
     CDS             complement(8271..9662)
                     /locus_tag="MJF80_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HBZ0281357.1"
                     /translation="MKRRLAGEWEPIKGVMLAWPPFLPHKYIVDLTQNYSVYMCVPNK
                     EEIEIAKYYLSKWGGNLKNLIFCIAPQGFDTPWVRDWGMHPIFDENGKLFLAGAEYKM
                     STPFVSINKPEIMFDCEHNLLTTNSYEQKEDQSQELIAKQIGLPFMKLPYALTGGNLM
                     SDGFNKLMSMHILQTENRAKGISDEEFFSMVARDTGMNEYYIFSNFADYGLQHIDCYL
                     KIIDEETLLVSRTPKEHYLYERYEKILEEVKKVETRYGRPFKIFRIDIVPFQKDSDEL
                     TAYVNSVILNSRVYVPMYGIEQDKIAIKQWQNALPGYEIKGYEFDFEKEPDIIKNRTG
                     YVRTGWGNEDVIHCRTRAIWDENMLYISVKRLEEIVSENDALEVTIQIVDYSKAGLDY
                     ENCRLFYRYYGFQTWESIMLEETTEAEIFFANMIGKSGDMIEYFVQAKSCSGMCETMP
                     PVAPKGTYKVTIK"
     gene            complement(9681..10220)
                     /locus_tag="MJF80_002708"
     CDS             complement(9681..10220)
                     /locus_tag="MJF80_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0281358.1"
                     /translation="MSLTIQKVTSYFKDLEKFEELNNEAFPDEERMSIQEMLNLVQSK
                     KIEVSALYDKQQFIGFYVLVIENHIGYILFLAISPEQRSKGYGGKALSLMKKQYPNCQ
                     IVLDMETIEDSAPNLQQRILRKQFYLQNGFYETEYLMEYKGLEMEVLCNQKTFQKNEF
                     ETLLKNLKIREIPFHLWKK"
     gene            complement(10241..11566)
                     /locus_tag="MJF80_002709"
     CDS             complement(10241..11566)
                     /locus_tag="MJF80_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009295908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBZ0281359.1"
                     /translation="MIQLSDNFNYKRLLKFTFPTIIMLVISSIYGVVDGYFVSNFVGK
                     TAFTAVNFIMPFLLILGCVGFLFGTGGGALIAKTMGEGKKEEANEQFSLLIVTSAGCG
                     IILAVLGIVTMPWIASAMGADGQLLTDSILYGCVVIIAIPAYILQCEFQCLFATAEKP
                     KLGLYVAIMAGITNIVLDALFITVFKWGLVGAALATAIGQYVGGIIPLLYFSRQNNSL
                     LKFKKPKFDCDVLKKTVANGSSELMTNVSTSVVSMLYNAQLLKYAGEDGVASYGVLLY
                     VALLFQAVFIGYSVGTAPIISYHYGAKNYDELKNLRKRSLIVIICFAVFMFIIGEFLS
                     KPIAFIFVGYDNNLMEMTLRAFMIYSFSFLFSGFGVFGSSFFTALNDGLTSALIAFMR
                     ILVFQVVSVLIFPIFWGVNGIWFSIVGAEILATIMTAFFIIKKQKKYHY"
     gene            complement(11563..12186)
                     /locus_tag="MJF80_002710"
     CDS             complement(11563..12186)
                     /locus_tag="MJF80_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HBZ0281360.1"
                     /translation="MKIITISREFGSGGRELGKQLADILNFDYYDKEIITAIARKKKI
                     DENYVSYILENHGWRNVPLTMCTSFTNILSMQSMKTDLLIEQSNVINQIASTGKDCVI
                     VGRNADILLKKYQPFNVFVCANMNFKIQRCIEKKLNNEHISKKELERKIREIDKNRAR
                     SREFFTSSLWGNQTSYHIIVNTSEWNIKHLSYAIANFSEYWFRRYEQ"
     gene            12278..13087
                     /locus_tag="MJF80_002711"
     CDS             12278..13087
                     /locus_tag="MJF80_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0281361.1"
                     /translation="MIKKLYKTSEFAELCGVKKHTLYHYDEIELLKPSITHENGYRYY
                     TIDQFEKFNIISVLKKAGTPLEEIKQYLNDLDTNTFLNVLHRKLMDLQEEVKKIEKMC
                     SMLRNVISTMEIHLNVNTEKIEIVNCEEEYLIATKIPEAVLTDEKTMLQGIGNHLQYC
                     LLHDYNFHVGEIVLEKNIKQGIFRESYYYSLIKNKVDDERLFIKPKGNYAVMYHQGSY
                     DSLYKTYQNMKNQLEEQGYHIVGNIYEEDLIDYLSEHNAENYIQKISIQVI"
     gene            13521..13883
                     /locus_tag="MJF80_002712"
     CDS             13521..13883
                     /locus_tag="MJF80_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281362.1"
                     /translation="MEIDYKAIGQRIKIARIKKGITQESVANIINMTPSHMSNVETGK
                     TKVSLPTLIAIANALSVSVDTLLCDNVLSSKVIFEKEAKDIFSDCDEYEIRSLVELLK
                     SAKTVIRKDREIRKQFEK"
     gene            complement(14233..14790)
                     /locus_tag="MJF80_002713"
     CDS             complement(14233..14790)
                     /locus_tag="MJF80_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0281363.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
     gene            15048..>15712
                     /locus_tag="MJF80_002714"
     CDS             15048..>15712
                     /locus_tag="MJF80_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBZ0281364.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNS"
BASE COUNT         5308 a         2793 c         1770 g         5841 t
ORIGIN      
        1 tgacaatata gaaattaacc atgtatttca ttatattaat aaaaaggata ccaaaaatgg
       61 tatccttcat aaatatttat atgtttattt ataaaaataa tcatatattt attactttac
      121 tacacctaaa caatattatt caagtgtatt catagagtta gaaaattctt gtcctgctgt
      181 aacaagatta gataacaata catcagctac tgcatcattt actgaactta ttatccattt
      241 attatccttt ttagaacatt caaactctac attttttgta acataagtct ctttaacgct
      301 tttttgtttt tctttcataa ttgatactaa catttcagtt gttttctcat cagttaagtc
      361 ttgtcctgaa aaactcatac caatcatctt tgtaaatgcc tcagctacta tttcttgaag
      421 taatggtgtt gaatctataa atttacattc tactttgatt gtagccttat cacctttaac
      481 ctcgctgtct tttattgtat atgtgatttt agatgcattc tgttttagat aatctaaaac
      541 atattgggca tttgggtctt ttgtatctaa ttcctttaat atatccttat atttttcgtc
      601 attattttcc attaccttat tcatgccttc aaagtcaaac tttttcgcac tgttaaaaaa
      661 actatcaatt gtatcttctg gttttgcact tgaacatgct gttaaagata ttgctaataa
      721 aaaagttgtt aaagctaata tgattttttt catatctcaa atcctccctc ttaatttgtt
      781 gatacataag ttattttaac atataatgag agtttttgaa taatatattt aaaatatttt
      841 ataaattttc attaaagtaa agtctatatt aaaacatatc tatattaaat tattgttcat
      901 atttacatca gtcatagtag aaaccataac agcttttatt gtatgcattc tattttcagc
      961 ttcttcaaat acctttgaat attttgaatt aaatacctcg tttgtaacct ctaattctga
     1021 taatccgaac ttttcataaa tctcctgagc aaccttagtt tctaaatcat gaaatgctgg
     1081 aagacagtgt aaaaatataa cattttcatt ttcagttttc tttatcatat ccatatttac
     1141 ttgataatct ttaagttgat ttattcttgc ttcatattgc tcctcttctc ccatagaaac
     1201 ccatacatct gtatatataa catctgcacc tttaacatca tcaatatttt cagtcaacat
     1261 tatatctcct ttggatttct tggctatttc cttcatttcc ttaaccaatt cttcatttgg
     1321 ccaaagttcc ttaggtgcta gtgctacgaa atgtattccc attttagcac atcctatcat
     1381 cagtgagttc cccatattat ttctagcatc accaacatat acaaatttaa ctttattcaa
     1441 atctttatct acattttcaa ttactgttaa aaaatctgcc agtatttgtg ttggatgata
     1501 ttcatctgta agtccattcc aaacaggtac accagaatat tttgctagac tttcaactgt
     1561 ttcttgttta aagcctctaa attctattcc atcatagaat cttcctagaa cttttgctgt
     1621 atctgataca gactcttttt tacccatatg acaatcattt ggacctagat aagttacatg
     1681 tgctccttca tcatgagctg ctacttcaaa tgaacatcta gttcttgttg atgttttctc
     1741 aaataataaa gctatatttt ttccttgtag caaatcgcct tttataccaa cttttttctt
     1801 agtttttaaa taacttgata aagttaataa atatctaatc tcttcttttg taaaatcctt
     1861 taatgtcaaa aaactcttac cttttaaact tatattattc atattaattc gccccttctt
     1921 ataaactttt taaattcaat taaacttgtt agcactttac ttttttattt acttctaatt
     1981 tttatcttta atcaactatt ttttgaatat ttagttaatt tctagtaata tatcttttag
     2041 gatttcaaga gcctcatcta tttccttata actgattgtt aatggtggta aaaatctcaa
     2101 tacatttttt ccagcagtca aaataagcaa tccttttttt ctagctttct cttctataat
     2161 actagattca atattagttt caattcctat cattagacca ataccttttg tctttaatat
     2221 aactttgtta ttaaaacttt caatagattt tcttatataa tttccttttt ctaatacttc
     2281 attataaaag ccatctttag atatctcatc taatacaact gaagctgcca ccatagctaa
     2341 taaattacct ccaaaagttg taccttggtc tccttttcct agtacttttg aaagtttttt
     2401 atttaccaat actcctccta ctggtattcc tgctcccaac ccttttgcta cggtaactat
     2461 atctggtttt acatcaaaat attcatagcc aaaaagttta ccagttcttc ctataccgca
     2521 ttggacttca tcaaaaatca ctacaatatc cttttcttga catattttta ctatttccaa
     2581 aacataatct ttttcaagaa cattaactcc accttcgcct tgaatcgctt caagaattat
     2641 tgcacaaact gtggaatcaa gattattttt taaatcttca atattatttc tttctatata
     2701 tttaaaacct tcgggaagag gataaaaata cttatggtat ttgtccatac cagttgccat
     2761 taaagacatc attgttctac catgaaaaga gtcttttaaa cttatgatag ttcctctacc
     2821 attaccatat ttgtcactac tatactttct tgctattttg aatgctgttt catttgcttc
     2881 tgttccagaa ttacaaaaat atactttagc cattccactt gaattaatta ttttctctcc
     2941 tgaatacaac actggctcat taaaatataa atttgaagta tgtaataatt tatctacttg
     3001 ctcttttaga gcattcttta aattactatt actataccca agagaagata ctccatatcc
     3061 acttgtaaaa tctatatatt tattgccatt agtatcaaaa aaacaacttc cttcaccata
     3121 atctatgaca aaattgggtt ggttatatgt atctataaaa tattcattcc attttgaaat
     3181 tgtattgtcg ttattcattc ttattctccc ctcttaaact tataatttta ttcagtttct
     3241 tttcttatta atgtacctat tccactatca gtaaatagct ctgttattat agaatgtgga
     3301 actcttccat ccaaaatatg taccctattt actccgttat ttaaggcatc tacgcatccc
     3361 tctattttag gtatcatacc tccagtaatt ataccttctt caaataattt atcaacatcc
     3421 tctaatataa cttctgttat taatgatttt tcttcatctg gctctcttaa caaaccaggg
     3481 acatctgtaa gaagtatcaa tttatctgca tttaactctt tagctatagc agaagctgct
     3541 gtgtcaccat ttatattata agtctctcca tcatctccaa cacctatagt tgcaattact
     3601 gaaatataac ctgactttag acaagattct ataagctcaa catttacttt tttgatttct
     3661 cctacaaatc caagttcata atttttatat gggtcacata aaatcatatt attatctatt
     3721 ccacaaagcc ctactgcttt tccacctttt gtatgtattt tatttactaa gtctttatta
     3781 acctttccag caagtaccat ttttacaatt tccatggtct cttcatctgt atatctaaga
     3841 ccatttacaa atttactttc tatatccact tttgctagca tcttattgat ttctgcccct
     3901 ccaccatgta ctaaaactat attgattcca acataactca ttaacacaag gtcttccata
     3961 acagattctt ttagaccatc ttttttcatt gcacttccac catacttaac tacaatagtc
     4021 tttccctgat gtttttctat atatggtaaa gcttctataa gtgtatttgc tttttctata
     4081 ttaatcatta aagtatatcc tcctaatttc tataacttcc atttattctt acataatcat
     4141 agcttaaatc acatccccat gcatttcctc tggcattgcc catatttaaa tctactaata
     4201 tacttatttc gtcatcttca aggatttttt ttgctttttc ctcattaaaa tctaatccat
     4261 ttccattttt acatacttca atatatcctt tcatactctc aaaaataata tcaacttttt
     4321 ccatatctat ttcttcacct gcataaccta aagcacacag tattctaccc caatttgcgt
     4381 cagctccaaa tacagctgtc tttactaatg aagaagatat aactgttttt gctaatttta
     4441 ctgcatgttc ttcacttaat gctccattta tatagcattc aattagtttt gtacatcctt
     4501 ctccatcttt tgcaacaagt ttagcaagtt ctatacagac atatgtaagt gcactcaaaa
     4561 atacttgata atgttcatct tttttgttta ttctatcatt ttttgctttt ccatttgcta
     4621 gaatcattac catatcatta gtacttgtat ctccatctac actaactcta ttaaaacttt
     4681 tgtcaacagc aatttttaaa gcttctttta gcaaaactcc atcaatattt gcatctgtag
     4741 ttataaatcc taacgttgtt gccatattag gatgtatcat gccagaacct tttgccatac
     4801 ctccaatagt tattttttta tctccataca ttattgctac tgctatttct ttttttataa
     4861 tatcagttgt cataatagct tctcttgctc caatatgacc ttgtttactt agattattca
     4921 ctaattcatc catattttct tctattaaat ctatatttaa tggctttcct ataacacctg
     4981 tagatgcaac caaaacatca ctttctttaa tatttaattt atcactggtg aatttagcca
     5041 ttttataagc atttataagt ccttcttttc cattaaaggt attagcatta gcactattta
     5101 ctatgatagc ttgtgctttt ccatttttaa tgtgttcctg tgtaacataa acaggattgc
     5161 cctttacttt attttgagtt actactgctg ctgcatcaca taaaacatct gaataaacca
     5221 aagctaaatc tcttttttct ttattttttc tcaagccaca gtgaatccct gaagcaaaaa
     5281 atccttctga aactgtaact ccacccttta taatctccat atttatcctc ctaattatat
     5341 gagtttagta ttttttattt ctaaaatgat ggtgcaatat tttttaaacc tgtattttct
     5401 tcaataccaa atattatatt catgttttgt atagcttgac ctgctgctcc cttaatcata
     5461 ttatctatag ctgaacagat tatcaatgta tctccatttt catgtaatga tattacacat
     5521 ttatttgaga atctaacatt ctttaaagaa gctaccttat ctaatggaag aatttctata
     5581 aattctttaa attcataata atcagttaat ttcctatgta tatcatttat agaaatatta
     5641 tcttcttttg tacaatatat agttgataag attcccctat ttactgggat taagtttggt
     5701 gtaaaaacta cacttacttt ttctttgcag gattcactta aattttgttc tatttctgga
     5761 gtatgtctgt gtttccctat tttatatgca gtgatatttt cattaacctc cgtaaagtga
     5821 ctactaatcg ataattctct accagcacct gtaagacctg atttagaatc aattattata
     5881 ttattatttt ttatcaattt agaacttagt aatggcatca atggtaaaga aatactagtt
     5941 ggataacatc caggatttgc aattatatct gcatctttta tatcttcttt gtatatttca
     6001 cttaatccat atacagcttt tttatgtaag attttatcta taaaagatac tccataccat
     6061 ttactatata cttcttcatc ttttattcta aagtcagcac ctaaatctat aactttcttt
     6121 ttcgatttaa ttgcttttat tacaaacttt tcactaactc catgtggaag ggctgtaaat
     6181 acaacatcac acatatctat aactttttca ttttctatac atatcatgtt atttttatat
     6241 cctatacttg gatatatatc aactatatct tttccaacat acgaatttga acctatcgct
     6301 gtcacttcga ctttatcatg attcattaat aatctaatca actctgcccc gacatatcca
     6361 gttgcaccaa taataccaac ttttatttta ttcattttgt tccctccatt tttatattca
     6421 aatagtctta aaatcctatc tttccacttt actatatgat aatcatttga taatacgtag
     6481 attaaaatac tctaattaaa acaaaaaacc ccttagcatt ataaatacta agaggcgatt
     6541 ttccgcggta ccactcttat tggtaaaatt acaataatca ttaaaactca aattaaaaag
     6601 tatctacaaa atgtacattt aaaattaaaa aagcctcttg gtcaaagacc aagagacgaa
     6661 tatacacccg cgttaccact ctaattgata agataatctt acccactcta atctcttaag
     6721 gcgagattca ccaattacca tactattact tctggtaatc tcctccaaag ctctattcac
     6781 aataaaattc gttactaggc ttccaccatt cccagctctc tatcaactag ctttatgcta
     6841 ctctcttttt cacaggattt caatttattt tcttattgtt ttatatgata gtttattttt
     6901 ttaactttgt caatataatt tttaaatttt tggaaatatt ttctatataa ttaatttcca
     6961 gcattttgta tctaaattga gttataaata tctacagtaa taggacacca tgaatttcct
     7021 gctgtaatat attttaaaaa agttctccct cttgaatcaa gtcattcaaa agctatacct
     7081 ttgtcaaaat tatttttcat ccagcgtttt ttagaaaaaa cacaattttc tccataaaag
     7141 agacatacat acaactattc tttcctaatg taatatatgt atgatttctt tgtcatttaa
     7201 ctttttcaaa aacgcaatta acaatggaat accgaacaac ccaataaatc caaagaaatg
     7261 taatcctgtt agcatactgg ctaaggccaa tacaggatgt aactccatat gtttaccaac
     7321 taattttggt tcaataatat tcctgataat agtaatcaca atatatagta gtgcaattcc
     7381 taatgccatt gaataatccc ctaaaaataa actgataata caccaaggta ttagaatact
     7441 tccagttcct aaaacaggta gtatatctaa aagagcaatg aataaagcaa ttattccagc
     7501 ataagaaata ttaataatag caaaaccgac atataattca gaaaaagtga tagcaaaaat
     7561 taaaagataa gaaaaaccac atttaataat ggtatcaata gtaaacgctt ttgcttctgc
     7621 taaaagataa cttttatttt taggtatctg tagcactata aatctcttta tcgttggata
     7681 atcaattgct ataaaaatag aagcaacgat tgtaataatt gttttcataa atagtaatgg
     7741 aacagacgaa acaatatcag taataccaga tacgatatta tttgaaagag aaactatgaa
     7801 aacactcatt tcattcatca atacaggcat atttgcttct attaattcaa taaacgaaaa
     7861 attgaatgaa ccagaaaata gttcgacttc ttttataact gtttcagtca aaggtattac
     7921 caattcctca aacatggttg gtaggaaatg aaaaatttcc tttattccat atattagata
     7981 tgtacctatt acacttacta ttattcctgc aattaaaaag aataaaaaaa cagatatggt
     8041 tgctacaata cctcgcttta ttagtaattt ttcacttatg tataagactg gtttattcaa
     8101 taaacttgct attaaaaatg caaaaacaaa aggaagtaat acagaaaacg catatttccc
     8161 taataacatt agtaagccta atatcactac caaataacag aaatttatta aaaatctctg
     8221 cttattttgg tatgatgtat gatcttgttc attcaaataa aatgcacctc ctactttata
     8281 gtaactttat atgtgccttt tggagcaaca gggggcattg tttcacacat tccagaacaa
     8341 ctttttgctt gcacaaaata ttcaatcata tcacctgact ttcctatcat attggcaaaa
     8401 aatatttctg cttctgtagt ttcttctaac ataatacttt cccatgtttg aaaaccatag
     8461 tatcgataaa acagtctaca attttcataa tctaagccag ctttactata atcaactatt
     8521 tgaatagtaa cttctaaagc atcattctct gaaacgattt cttctaatct tttcacggaa
     8581 atatagagca tattctcatc ccaaattgct cttgtcctgc aatgaataac atcttcattt
     8641 ccccagccag ttctcacata tcctgtacga ttttttataa tatctggctc tttttcaaaa
     8701 tcaaactcat atcctttaat ttcatatcca ggcaaagcgt tctgccattg ttttattgca
     8761 attttatcct gttcaattcc atacattgga acatagacac gactatttaa aatcacagaa
     8821 ttaacataag cagtaagttc atcactgtcc ttttgaaacg gaacaatatc tatacgaaag
     8881 attttgaatg gacgtccata tctggtttct acctttttta cttcttctaa aattttttca
     8941 tatctttcat acaaatagtg ttcttttggc gttctgctca caagtaatgt ttcttcatca
     9001 attattttta aataacagtc aatatgttgt aaaccataat cagcaaaatt agaaaatata
     9061 taatactcat tcattccagt atctcttgcc accatagaaa aaaattcttc gtcagaaatt
     9121 cctttcgcac gattttctgt ctgtaaaatg tgcatagaca tcaatttatt gaaaccatct
     9181 gacataagat ttccgcctgt tagtgcataa ggtaatttca taaatggcaa accaatctgt
     9241 ttcgcaatca actcttgtga ttgatcttct ttttgttcat aagagttagt agttaatagg
     9301 ttatgttcgc aatcaaacat gatttctggt ttatttatgg atacaaatgg tgttgacatc
     9361 ttatattctg caccagccaa aaagagtttt ccgttttcat caaaaatagg gtgcataccc
     9421 caatcacgaa cccatggtgt atcaaatccc tgtggagcaa tgcagaaaat caaatttttt
     9481 aaatttccac cccacttact taaataatat tttgcaattt caatttcctc tttatttggt
     9541 acacacatgt atacagaata attttgcgtt aaatctacaa tatacttatg gggaagaaaa
     9601 gggggccatg caagcatgac acctttaatc ggttcccatt ccccagctaa acgtcttttc
     9661 atttctaata cctcactttc ttactttttc cacaaatgaa atggaatttc tctaatttta
     9721 agatttttca acaatgtttc aaattcattt ttttgaaagg ttttctgatt acataaaact
     9781 tccatttcta gtcccttata ctccatgaga tattctgttt cataaaaccc attctgaaga
     9841 taaaattgct tccttaaaat tctttgctgt aaattaggag cactgtcctc tatagtttcc
     9901 atatcaagaa caatttgaca attgggatat tgttttttca taagtgacag tgcttttcca
     9961 ccatatcctt tagagcgttg ttctggagaa attgcaagaa ataatatata tcctatatga
    10021 ttttcaatca ccaaaacata aaaaccaata aattgttgct tatcatataa agcagatact
    10081 tctatttttt tactttgaac taaattaagc atttcttgta tagacatacg ttcttcatct
    10141 ggaaatgcct cgttattaag ttcttcaaat ttttctaaat ctttaaaata tgaagttact
    10201 ttttgtattg ttaagctcat atagatacct ccaaattgat ttaataatga tattttttct
    10261 gtttttttat tataaagaaa gccgtcataa tagttgccaa tatttcagca ccaacaatag
    10321 aaaaccatat cccatttact ccccagaata tagggaatat aagtactgat acaacttgaa
    10381 atactaaaat acgcataaaa gcaataagcg cagaagttaa accgtcattt aatgcagtaa
    10441 agaaactaga accaaaaaca ccaaaaccag aaaataaaaa ggaaaaagag taaatcataa
    10501 atgctcttaa tgtcatttcc attaaattat tatcgtaacc cacaaaaata aaagcaatcg
    10561 gttttgataa aaattcgcct attataaaca taaatacagc aaaacaaata attacaatca
    10621 aacttctttt tcttaaattt tttaattcgt cataattttt tgctccataa tgataactga
    10681 ttataggggc agtaccaaca gaatacccaa taaaaacagc ttgaaatagt aacgctacat
    10741 aaagaagaac accataggat gcaacgccat cttcgccagc atattttagt aattgagcat
    10801 tatacagcat actcacaaca gatgtggaaa cattagtcat tagttctgaa gaaccatttg
    10861 caacagtctt tttcaaaaca tcacaatcaa atttaggttt cttaaattta agcaaactgt
    10921 tgttttgtcg gctaaaataa agtaatggaa tgataccacc aacatattga ccgattgcag
    10981 ttgccagtgc tgcacctacc aatccccatt taaaaacagt gataaataat gcgtctaaaa
    11041 caatatttgt aattccagcc ataattgcaa catataatcc aagttttggc ttttctgctg
    11101 ttgcaaataa acactgaaat tcgcactgca aaatataggc aggaatagcg ataattacca
    11161 cacatccata aagaatacta tctgtcaata attgaccatc tgctcccata gcagaagcaa
    11221 tccatggcat agttactata cctaaaacag ctagaataat accacaacca gcagaagtaa
    11281 caatgagtaa cgaaaactgt tcgtttgctt cttctttttt tccttcaccc attgttttag
    11341 caattaaagc tccaccacct gttccaaaaa gaaagccaac acaccctaag attagcaaaa
    11401 atggcataat aaaattgaca gcagtaaaag cagttttccc tacaaaattt gaaacaaaat
    11461 atccatcaac aaccccataa atagaactta ttaccagcat aataattgta ggaaatgtaa
    11521 attttaataa gcgtttataa ttaaaattat ctgacaactg tatcattgtt catacctcct
    11581 aaaccaatac tctgaaaagt tagcaattgc ataagaaaga tgttttatat tccattcaga
    11641 tgtattaact ataatatgat aggaagtttg atttccccat aaggagctcg taaaaaattc
    11701 tcttgatctt gctcgatttt tatcaatctc tcttatcttt ctttctaatt ctttttttga
    11761 aatatgttca ttgtttaact ttttttcaat acagcgttgt attttaaagt tcatatttgc
    11821 acaaacaaaa acgttaaatg gctggtactt ctttaataat atatctgcat ttctaccaac
    11881 aataacacaa tccttacctg tactggcaat ctgattgata acatttgatt gttctattaa
    11941 caaatctgtc ttcatggatt gcatagacaa aatattagta aaagaagtac acattgttaa
    12001 aggaacgttt ctccaaccat gattttctaa tatgtaggaa acatagtttt cgtcaatttt
    12061 tttctttcta gctatagcag tgataatttc tttatcgtag tagtcaaaat ttaaaatatc
    12121 agcaagttgt tttccaagtt ctcgtccacc acttccaaac tctcggctaa ttgtaataat
    12181 tttcataata tcttttcctc acatttcatt gaatataaaa gctttctatg ttatcctaaa
    12241 gtatctggta accctaaagt caataggaga tttaaaaatg attaaaaaat tatataagac
    12301 aagtgaattt gcagaattgt gtggggtaaa aaaacataca ctttaccatt atgatgaaat
    12361 tgaattatta aaaccgagta ttacccatga aaatggctat cggtattata caattgacca
    12421 atttgaaaaa tttaatatta tttctgtgct aaaaaaagca ggtacacctt tagaagaaat
    12481 aaaacaatat ctcaatgatt tagatacgaa tacctttctt aatgtacttc acagaaagct
    12541 aatggatctt caagaagaag tgaagaagat tgaaaaaatg tgttctatgt tgagaaatgt
    12601 catttcaaca atggaaatac atctcaatgt aaacacagaa aaaatcgaaa ttgtaaattg
    12661 tgaggaagaa tatctgattg caactaaaat tcctgaagct gtactaacag atgaaaaaac
    12721 aatgttacaa ggtattggaa atcacttaca atattgttta ttacatgatt ataattttca
    12781 tgtaggagaa attgttcttg aaaaaaatat aaaacaggga atttttagag agagctatta
    12841 ttatagcctt attaaaaata aagtagatga tgagcgcctt tttattaaac caaagggaaa
    12901 ttatgcagtt atgtatcacc aggggagtta tgacagttta tataaaactt atcaaaatat
    12961 gaaaaatcag ttagaagaac aagggtatca tattgttgga aatatttatg aagaagattt
    13021 aattgattat ttatcagaac ataacgccga aaactacatt caaaaaattt ctatacaggt
    13081 tatttgaaaa tataaaaagc gtgtatatct caaatcacat gagaaataca cgcctttttt
    13141 aatatctcgt attgtctata ataatttgct taaaatgtcc ttttttatct tctaaagttg
    13201 gtatcttatt tccaaatttt tgtagaaatt caggttcatt gctattattt gtttcctcaa
    13261 tgcaaactgc atttcctcat gaatttcaga tacagaaata gccttttatt ttcgttaacc
    13321 atttaatcag ttttttacta tctttttata aaatcttaac atggaggctt tatgcctcca
    13381 tttcttttgt agttgtctta aaagtagtgc aaattttttt cttaaatata tattatataa
    13441 attattcatt tatatagagt attatattat gctctgtaaa tagttacaat atttaaaaga
    13501 atgaaaggag aacaaaaact atggaaattg attataaagc aatcggacag cgaattaaaa
    13561 ttgcaagaat taaaaaaggt ataacacaag aatccgttgc aaacattatc aacatgacac
    13621 catcccatat gagcaacgtg gaaacaggaa aaacgaaagt cagcctgcca accttgattg
    13681 cgattgccaa tgcactttct gtatcagtcg atactctctt atgtgataat gtactttctt
    13741 ccaaagtaat ttttgaaaag gaagccaaag atattttctc agattgtgat gaatatgaaa
    13801 tccgttcttt agttgaactg ctaaaatcag caaaaactgt aatccgtaaa gacagagaaa
    13861 tccgcaaaca atttgaaaaa taaaaccaca gcttcaaggt ataaattgat atgtgttgtt
    13921 agctaaggtc ttttcttttt ttatataatc atttttatat aatcaaataa cctatccaat
    13981 aattttccaa ttactgtcct tatgaaatat caatttatac tatgatacct gtgtcgcttt
    14041 tgtctgatta tcaaggaatt ttacagctac cttttcatta tcaacgacaa cagtaaaaat
    14101 agggtttacc aattcagaaa gtaaatattc ttgaaatata ctattttcct ttcataaaaa
    14161 tgataaaatt ttattttaca tcccacgtaa tgaagtgttc aataaataag ttaaaataat
    14221 ttatttacct ccttaatcat attttttatg aatacctaat aacacagtat caatagaatc
    14281 gactagacct tttatagctt cttctttact caaaagtgat ttcccaagtt gcaaagtcga
    14341 ataaccatga agtacactca ttattaagtt taatacttca cctgtattct ttgtatttag
    14401 attacaagaa agaataagtt tttctaataa ggatttatat tcatcaaaga tttctaatgt
    14461 ttcattattt ccatgccaag tagcccactg aattgtttca taaacaccag gattagaaat
    14521 tataaaatta aagtatgcta tacttataca ctttatagct gtatctccaa aatttccaat
    14581 agccgccttt atcatttgac tattcatctc tctcattcct ttatgagcta cttcaagtaa
    14641 taaatcatct agactttcta tatgattgta caaagatggt gtacgtattc ctagcttttc
    14701 tgcaataact tttaaagaaa ctttgttaag cccatcttta ttaactatat ctgatgtagc
    14761 ttgtataact atttctctac taacacgcat gattttccct ctttcattta aaattaatta
    14821 ctttattagt ttagctaatc taattagttt cgtcaattat tgtaatttat aaaattcttt
    14881 atactattat aaaatcttga cagtttaatt ataatttaat ataatactaa ttgaattagt
    14941 tttaatacta atataattag tattatatta aattctatat tacaacatta ttttatagtt
    15001 tgattaattt aacaatcact aatatatcaa actgaaagga gatttttatg gaaaaaaaat
    15061 atttcgaaat tcatggagtt ccttcaatcc tttggggaaa tccttcaagt aaattatata
    15121 tttacataca tggacaatgt ggcaataaag aggaggctga agcttttgct aatattgcag
    15181 ttccacatga gtgtcaagtt ttaagtgttg atttgcctga acatggagaa cgtaaaagag
    15241 aaaaaaattc atttaaccca tggcatgttg ttccagaatt agtatctatt atggaatatg
    15301 caaagtgcca ctggaatcaa atttcgttat atgcaaatag cattggagca tggtttagta
    15361 tgttaagctt tgaaaaagag aacttaagag agtgtatttt tgtatctcca attctagata
    15421 tgcaacagct catttcaaat atgatgcttt gggcaaatgt atcagaagaa cagcttaaac
    15481 atgaactaat tatcccaact tcatttgggc atactctttc atgggaatat ctacaatatg
    15541 taaaacaaca tcctatcact caatggaatg taccaacaaa aatattatat ggtaaacatg
    15601 atgaacttac tgaaatcggt gttgtagaga aatttgtaga aaagtttaat tgcaacctta
    15661 ctattataga aaatggcaaa cattggttta acacaccaca agaattaaat tc
//
LOCUS       DAGSWI010000096        15535 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.096, whole genome shotgun sequence.
ACCESSION   DAGSWI010000096 DAGSWI010000000
VERSION     DAGSWI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15535
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.096"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            213..1148
                     /locus_tag="MJF80_002715"
     CDS             213..1148
                     /locus_tag="MJF80_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBZ0281365.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(1240..3111)
                     /locus_tag="MJF80_002716"
     CDS             complement(1240..3111)
                     /locus_tag="MJF80_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBZ0281366.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            3301..4227
                     /locus_tag="MJF80_002717"
     CDS             3301..4227
                     /locus_tag="MJF80_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBZ0281367.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            4366..4512
                     /locus_tag="MJF80_002718"
     CDS             4366..4512
                     /locus_tag="MJF80_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281368.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(4617..5141)
                     /locus_tag="MJF80_002719"
     CDS             complement(4617..5141)
                     /locus_tag="MJF80_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0281369.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(5194..5418)
                     /locus_tag="MJF80_002720"
     CDS             complement(5194..5418)
                     /locus_tag="MJF80_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281370.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            5615..6739
                     /locus_tag="MJF80_002721"
     CDS             5615..6739
                     /locus_tag="MJF80_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBZ0281371.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGILDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(7213..8037)
                     /locus_tag="MJF80_002722"
     CDS             complement(7213..8037)
                     /locus_tag="MJF80_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281372.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(8385..9440)
                     /gene="ispG"
                     /locus_tag="MJF80_002723"
                     /gene_synonym="gcpE"
     CDS             complement(8385..9440)
                     /gene="ispG"
                     /locus_tag="MJF80_002723"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBZ0281373.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(9623..10627)
                     /gene="rseP"
                     /locus_tag="MJF80_002724"
     CDS             complement(9623..10627)
                     /gene="rseP"
                     /locus_tag="MJF80_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBZ0281374.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(10640..11794)
                     /locus_tag="MJF80_002725"
     CDS             complement(10640..11794)
                     /locus_tag="MJF80_002725"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBZ0281375.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(11958..12161)
                     /locus_tag="MJF80_002726"
     CDS             complement(11958..12161)
                     /locus_tag="MJF80_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0281376.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            12498..13079
                     /locus_tag="MJF80_002727"
     CDS             12498..13079
                     /locus_tag="MJF80_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281377.1"
                     /translation="MLYLLKINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
     gene            complement(13167..14978)
                     /locus_tag="MJF80_002728"
     CDS             complement(13167..14978)
                     /locus_tag="MJF80_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBZ0281378.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATVLSKTFNIKETCARICSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
BASE COUNT         5340 a         2517 c         1591 g         6087 t
ORIGIN      
        1 gcgtttcata aatagctata tataaaataa taaataagga gagtgatggg caatggatac
       61 tagtccgttg cggagagaat tttttacatt ttcatcactt aataatatct aaaatcaaaa
      121 ctaatacata tattcagtta tataaattag ttaattaata gatactatta aatgatgaga
      181 tttatataaa actgaataaa ggatgtgttg ccatgataag atattacaaa actatagatt
      241 caaaattaga gaaacttagt ttttttgaag atggctgttg gataaatctt gttgaaccta
      301 atcatagtga aatcaacgaa atttctaatt tattaaatat agatgtagaa agcatagaat
      361 ctgcactaga tgaagaagaa cgctcaagaa tagatgttga agacaatcat actcttatac
      421 ttatagatat accagtagac gaaagtgact ctaattcttc tcattatact acaataccct
      481 taggtattat actaactgaa gaagctattg taacagtttg tgatgcacaa actaagattt
      541 taaatgactt tatagttggt catattaaag atttttttac gtttaaaaag actcgctttt
      601 tgcttcaaat tcttcacaaa aatgcagctt attacttgca ttacttaaga aaaataaata
      661 aaatgacaat cataatagag cgagaaattt acaaatcaat gaaaaacaaa gaattggttc
      721 aattattgga attagaaaaa tctttggtat acttctctac ctcactaaaa tctaatgaac
      781 ttgttttaaa caaaatggtt agaactgctg gtataaagaa gtatccagat gatgaagacc
      841 tattagaaga tgttatagtt gaaaatagac aggctcttga tatggctaaa atatatggag
      901 atatattgag tagaattatg gatgctttca gtgcaattat aagtaataat cagaataatg
      961 ttatgcaaat tttaacagta gttacactaa ttttctcaat acctacaata atatcaggat
     1021 tctttggtat gaatgtcatc aacatgccat tttctaataa cccaaacgga ttttggataa
     1081 tacttcttat tacctctata atatgtatag tgattacatt tttcatgtca agaaataagc
     1141 tattataaaa tattaataaa aaagccatta gaattaattg atttacattt atcctaatgg
     1201 cttttatata tttaaatcta tataatctta taatactttt taactatcat gttcttcatc
     1261 tataatcatt tcagtttcaa gcccttctag cattgaatct aaactttctt ttatatttag
     1321 tatataaatt ggtacatact cttcttcatc tattacctct tcttgcaact taaatggagg
     1381 aattaatact tcatcaaagt ctatatttaa atttggaaag ccttctacta tctcttcaca
     1441 attatcgtac tcttcatttg ctaaagaaat gtacatatct aggctctttt tataataaag
     1501 acctgcaagc atatactcac acttcttact tccatattta ataccttctt tataatattt
     1561 ctcagcttct gcgtcatcat ttaattttaa ataaatttga cctaaattat atattgattc
     1621 aacacaacct tgtgctctag ccttttcata ccaaaattta gaatttacat aatctttata
     1681 gaacatatca taaattaatc ctaccatatg ttgagcatac acattatttt cattggcagg
     1741 tgtatcaaac atcattttag actcttccaa tttaccttct ctaaaataaa ttctacctag
     1801 atgtatacga gcattcatat ggtttttgct acttgccatt tcaaaatact ttctagcatt
     1861 ttttgtattt cctaagctct catatataca ccccaatcta taactagacc tcgtatctcc
     1921 attttcagat gctttcttat accaacttat tgcttcttca atattattta aatcttcata
     1981 gatatttcct aatttgattt gacaagatac attatttgga atcctcgaat aatacgaaat
     2041 cgctttttct atatcttgat tctgataata taaatcccct agattttcaa gggaactttt
     2101 gcatccaaca gctatagcat cctcataata tttaatagca ttttcataat ctttttcttc
     2161 aaaatatata cctgccagat tattttttgc ttctgttata tcatcttcag cagccatttt
     2221 ataataagtt tttgccttct ctatctcatt ttctctatta tataaattag caagtctata
     2281 cttagaaagt gtatgatttt tttctattgc tttctcataa taataaattg catctcccag
     2341 ctcttctttt ctatcatata catatgcaag cctatactca gacttcacat catctctatc
     2401 agcactcttt ttataatact tctctgcatc ttctaaatta tcatttattt ccataatcat
     2461 acccaagtta aagcaacctt tcatatgacc tgacttgtac aactgtttat ataaacttac
     2521 tgttgattta ttatctttct tttttccatt taatattgct aaattatatt ttgcatcatc
     2581 atctaaaagc acagaacctt ttttatagta ttcatatgct tcatcatctt tctccaaaaa
     2641 agtatataat acccctaaat taaaacaagc tttacctaaa ttcttgtctg ctgctgtttt
     2701 taaccatttt tcacacctta caaaatcttt cattttaaaa tggcatacac ctaaattatt
     2761 ttgtgcttgt aaataaccta aatttgctat cttttcatac caatatatag catattcata
     2821 gccatctaac ttatagtaca tattacctaa atcaaaagca gcatcttcac ttccactttt
     2881 atatgccatt gaataccact tttctgcttc tttaaattgt tttttacttt catatatatt
     2941 tcctaaagta tattgagatt ttacgtaatt atccaatgca gcactcttat acaactctat
     3001 tgcgctttcc atcttaccca tcttttcaag gagggcaccc ttagtatgta cgactttcgg
     3061 gtcattagat atatttttta ttttttttat agaaaatttt gtatttttca ttattcgcta
     3121 cctacccatt ttataaaata tattctctca aacattatta tacattataa tctacaatta
     3181 atgctataat attgaatttt taccttttgt cactatattg taacattaaa ttttaaattt
     3241 tataatcatt agattttatt atgtaattgg tatataatgt atatataggg gggacatttt
     3301 atgaatttca gcaatatttt tatccaagtt gcagttttat ttataattat tttagttgga
     3361 tattttgtca gaaaattcaa cttattagat gaccactgca catctaagct ttcaactttg
     3421 acaatgacag tttttttacc atcaatgata attagctcca tgcaaattaa ctttgatagt
     3481 aaaatgatac aaaaaatact attgttatta tttacatctt tgattatgta tatagtttca
     3541 attataattg catttttatt gaaatacatt ttaaaatgta atgataaaaa agatttggga
     3601 atataccagt atatagttgt tttttcaaat gttgctttta tgggttatcc tgtaattgaa
     3661 gcagttttag gtcatgaagc aatattctat actgcaatat tcaatttgcc ttttaactta
     3721 ttttctttca ctttaggaat ttatctttta tctaaaggca gtacaagtag aggtttttca
     3781 ataaaatcac ttataagccc tgctacaata gctattgtta ttggactttt tttatttgta
     3841 acaggactta ggttacccca atttataaac gagccacttg agatgttggg aagtataact
     3901 acaccaatat ccatgattat cattggaagc ttacttgcta attcttcagc tctcgattgt
     3961 tttgtcaaca aaaaattata tatagtcact tttataagat tattggtact tcctgtgatt
     4021 gtgtatttta ttctaaaagg atggataaat gataagatga ttttagctat accagtagta
     4081 atttcttcaa tgcctgctgc tgcaaatact gctataatgg ctaatcaata tgactccaat
     4141 ataacactag cttcacaatg tgtgtttttt acaacactat tttcagttat atctatacct
     4201 tttataagta ttttactctt atcttgattt atttttttat gtattatata ataacatatt
     4261 caataaatta caaatataaa cattttattg tagtatattg atggaatttt ttcctagata
     4321 tttgtcaata atatctatat gtattataga ataggaggct taaatatgga tatggattta
     4381 ctctttaaaa tagtatttgc tatagttgct ataggtgttt ctgtaaaaat cattaaaact
     4441 gttgctagtt tagcttttaa aatagcgttg atattgctaa taattttatt tgtatataga
     4501 atgtttttgt aaacatttaa aaataagttt tttaacacaa tagagaagta aaatagatta
     4561 tctttaatgt aaatttttta ttaaagacac tctattttac ttctctatta gcttatttaa
     4621 attaaatttt tattatatct tcccataata taaacatctt caaattttcc atctctgatt
     4681 acagcatatt ttttagttcc ctctatttca aaacctagct ttttatataa atttaatccc
     4741 ttttcattat ccacaattac ttctaatcct actctagtta acataatcca attatctgct
     4801 atatctaaaa gttgttccat aagcttctta cctactcctc ttccctgata attctctgca
     4861 acacttattc ctatttctgc gctatgtctc aatcgtggag aagaatttac atgtaaccca
     4921 gcaaaaccta ttacatcttt ttctccatta ttattagttt cagctacaaa catataatca
     4981 ttttctccca aagaagctaa catagcttca gttttatcga ctctgtcact cattaatgcc
     5041 aaagtatttc tcataacact gtatgttctt ctaatttcat taaaagcttg tgcatcttct
     5101 cttcttatag gtcttattgt tatatctata ggcttactca tattggattc cccctcaaac
     5161 ataatcttta tttttaatta aaattttaca tctttatttt atactgttca aatctatgta
     5221 tgcttctacc tgaggtacat atatcatatt atcttttata tgtgtacttc caaattcttt
     5281 taattctgta ccttctttat ccttagttat tttatatcta atggattcca taccttcaac
     5341 tacttcagca tgaaaaaaca taccttcata ttgtttaaat ctaaaattaa aattgtcttt
     5401 agtaagtttt attatcatat ttttatctac tccttatata attgatattt ttcttattat
     5461 tagttgtata tatactgctt attcatatta atatattata acttatcatt tatttagtat
     5521 aaagttataa atagatatat ttatacttat tttacaatct ttcttcaatt gatatataat
     5581 atgtttatat aaactaatgt atgtgaggtg aaatatgagc aaaaaaacaa aatatttttc
     5641 tttaattctc tttcctattg ctttgctttt aaattttata gccagtaaag ttccaaatat
     5701 tgttgaaaaa tattactccc aattcataga taaaataatt gtacaaatat tgagtaaagt
     5761 ttctggaatt ttcccatttt ctctctatga gattacaatg tacataatag ttatatctat
     5821 cttcttattt ttatgttgta ctttatctac aatttttaat aagaaaaaaa aattaaacat
     5881 atatttaaaa aattccattt taaatatatt atccattgta tcaatagtct actttctttt
     5941 tgtagtgctt tggggaataa actataatag gattccacta gaaaccacac ttataaataa
     6001 ctataattta aaaaacaata cctctatcca atcaaaacaa tatagtgtta aagagcttac
     6061 taaattatat aaattccttg taattaaagc aaatgaaact agaaaactca cattacaaga
     6121 taaaaatggt attgtaaaat ctaatacaga ttataatggt gtcataaata gagctcaact
     6181 aggatacgat aatatcttgg atatattacc tagtgttagt ggtagttatt ctaagcctaa
     6241 gtatattatt tcatcaaatc taatgtgcta tacaggaata actggtatat actttccttt
     6301 tacaggtgag gcaaatgtaa atatagcaat acctgactta tatattcctt gtacagttga
     6361 acatgaaatg gcacatcaaa ggggatttgc tagtgaagat gaggctaatt ttatagcgta
     6421 tcttacttct ataaaacacc ccaatattga ttttaactat tctggatata ttttagcttt
     6481 aaattatact gcttctgctt taagtaaggt tgattataat gcatatgttg atatttctgc
     6541 tggcatttta gattctgtcc gtagagattt aaaaaatgaa agtgaatttt ggcaaaaata
     6601 tgaaggtaaa attaatgaaa tttctaatga gtttaacaat tcttacttaa aagctaatgg
     6661 cgttactgaa ggtactcaga gttatggtaa aatggttgat ttattgctaa cttattatga
     6721 attatatcca tataactaaa cactacaatt caaaatttta ttcatataca gctgatatta
     6781 aatcagctgt tttctattta aataaattat ttaacttcaa gcaatcttat ataatttgat
     6841 agtacttata ctatctgtat aacatatcaa ttctaactat tttagttaaa acatttctat
     6901 tatctttttt aataaatgtt ttaactaaaa tttttaaaac ataaaacttc cacattattt
     6961 cactatctat ataaaaatct aaaatttcac atataaaatt acataactaa aaaatcataa
     7021 atttatagaa aacatatatt caaaataaaa aagcatgtat caaaacttga cacatgctta
     7081 taatcttatg tatatatatc attttgtcac aataaaaatt tacttgtaac actgatattt
     7141 ttaaacaaaa tagtccttat ttaattaaat taacattaat taaattcgta ctcattttgc
     7201 tatcattaaa cattacttat agtaattttc taatacacta tttgaaactt ccttcccttg
     7261 aaaacttttc aaccaattct catatgctga gtttttccat acactaccat cattaaatgt
     7321 tacttgtttt acacaagata taatgtactt tacaccatgc tctccagttg attgtttcca
     7381 tttatcaaac aatagaaagg catcattttg attcttacct ggtgctattg cattttgctt
     7441 tgcatattct ttctccattt gtttccattc tttcaaccaa ttttctaccc atgccttgcc
     7501 ttgttgtttt tcaaacatac ttattatatc ctgtggtggt gattgttgca tttttctata
     7561 agtatgtgaa tatgattttg gagacacagg agatatgcct gtaacaatac catagtctac
     7621 accagctggg ctaaatccaa cacttcctac ttcgccatct gctgccacat tttgagcatc
     7681 ccaatatagc tcaagtggtt ttccattttt atcatatgct aagcatgtaa tttcataatc
     7741 cataattgcc ttttcactgc tatttgttaa gcttataaca ttgctaggcc aatgatatgg
     7801 agtatcatca tactgtattt tgctattatt tataaataat ggaatattct cattataaac
     7861 aagaccttta ctactttgaa tatctgatac cttctttgtg acagtactta ccgtatttgc
     7921 taagtataca tttgtttttt ctgaattggt cgtactaaga gaaaatgttg ttgtcgttcc
     7981 agtaatcagc aaagcaacaa ctaaaatagg taagctagat atttttttat atctcacaat
     8041 tcttcacctc tttttctatt aacttataat aaaacaattt accaaaacat taaaatctat
     8101 gttaacataa tttaccaact gttctagtaa ctatatccta tcatagttac tatcaagttt
     8161 caatactatt gatggaaatt ttcactatat taaatctttt tttaatttta taaatttaca
     8221 atatgattta actcatacat atatagtcaa attagaatat aagtttctta catttaatga
     8281 agtttataaa ataaaaaatg actagataat taaatactgt aaataaaaac ttattacaaa
     8341 agtatttaaa aatctagtca attaatatat atatgaattg tatactataa tttttcaatc
     8401 tcttctagca actcatcaac taatttgtta ccatcaatca ttctaactat ttcacccttt
     8461 ttaaataaaa gaccttcacc tttaccccca gcaataccta tatctgcctc tctggcttca
     8521 ccaggtccat ttacagcaca gcccataatt gcaacagtga tatttttttc catactacca
     8581 atcttttcct caacctcatt tacaacacta attaagtcta tattacatct accacatgta
     8641 gggcatgata taactttaat cttatcattt aaaagtccca aacttcttaa aatctgttta
     8701 ccaacaataa cctcttcaac agggtcgcct gttaaagata ttctaacagt atctccaatt
     8761 cctttaagaa gaagtgctcc tacccctata gaagacttta ttgttccttt gtgaacactt
     8821 ccagattctg taattccaat atgaagagga taatcaactt tttttgatat taattcatat
     8881 gcatcaacag ttttataaat atcggatgat tttagagata taactatatt atgaaaatct
     8941 aagtcttcaa gaatcttaat atgtcccatt gcactttcaa ctaatgcttc agcagttgca
     9001 gagccatact tttttaaaag ttctttctct aaagaacctc cattaactcc aattctaatt
     9061 tttaaatttc tttccttgca tttctcaact accatcttaa ctctctctat acttccaata
     9121 ttaccaggat ttattcttat tccatccaca ccttgttcta tagcttcaag ggctagctta
     9181 taatcgaaat gtatatctgc aataattggt atatttacac tagactttat ctttgctata
     9241 tttttagcag ctttaatatc aggaacagca actctaacta tatcacaacc agcttcctct
     9301 aatctcttta tttgagctat tgtagcatct gcatctcttg tatcagtatt tgtcatagat
     9361 tgtatactta taggattttc tcctcctatt tttacagttc ctacacttac ttctctcgtc
     9421 tttcttctct cgtaactcat attccacctc tatatattat tttatattga atctctttaa
     9481 atattaaaat attataacct acatgtatat tataaattaa aaagctatct agttaaataa
     9541 gaataaattt catacataaa aattaattta ttcttattta aaactacata gcctattttc
     9601 ttaaaatctc taaaaaacta aatcattgaa ataatcttag tatatcttta tatgtgataa
     9661 aaagcataaa taacattaaa gctccaaaac caacaacatt tatcatgcct tctttatttg
     9721 ggtcgagttt tttaccacct ctaacagctt ctagtaacag cataagtatt ctccatccat
     9781 caagtgctgg tattggtaat agatttacta tacctaaatt taagcttatg acagctgcta
     9841 aatacactac atttatgatg cctgtacgag cagcatctga taccatacca ataacaccta
     9901 ctggtcctgc tacagcattg ccaatccctc ctggtacacg tcctgtaatc atttgaccta
     9961 gaaatgttag catctgtttt gtcatatcta cagtagtttt acatgcattt actattgacc
    10021 ccaaaaagtc tttttctctt tgtggttgaa tccctatttc atactttcca ttgttatttt
    10081 taggtgttac atttacaact ttatcacttc catctctagt tatactaagt tttaattctc
    10141 caccagaaga agtgtttatt atatttgcaa cttcatccca agtcttaaca gaattaccat
    10201 taattttatt tatcttatct cctacttgaa gccctacagc ctgtgcagga gtatctggca
    10261 ttatctttcc taatttagtc gttggagaac ctatatacaa aaatactgga actaaaagta
    10321 ttgctgctaa tattatattg aatattggcc ctgctacaat agtactaaat ctttgtaata
    10381 tagacttgtt tccaaaagaa tttggactta tttgttcttc atcttcacct tccatgctga
    10441 catatccacc aattggaagt gctcttatag aatactctgt atcttttttt acactatata
    10501 tcttaggccc catacctata gaaaactcat ttactttgat accacttctt tttgcaaata
    10561 taaaatgtcc taactcatgt attaatacaa ttatactaaa tagtattaat gcggctatta
    10621 tagtcataaa ttcaccttcc tattttttta tcagatttgc aacataagct ctactccatc
    10681 tatctgtttc taagatttct tctaatgttg gttcacttat actactatga acttctaaag
    10741 tcttctctat gtaatatggt atgtcataga aaccaatttt atcctctaaa aattcactta
    10801 ctaaaacttc atttgctgaa tttaaaacag aagaataagt tcctcccatt tttaaacact
    10861 catatgccaa ttttaaacat ggaaaagttt ctaaatctgg ttcttcaaaa gttaaagtac
    10921 taaattttga aaaattcatt ctttcaaagc tactttccat tctatctgga tatgtaagag
    10981 catactgtat tggtagtctc atatctggac atcctaattg agcaattatt gagctatctg
    11041 tatactgcac cattgaatga attatgcttt gtgggtgaac tactacatct atattttctt
    11101 gttctactcc aaataaccat ctagcttcta tcacttcaag tcctttattc ataagcgttg
    11161 aagaatcaat acttattttt cttcccatac tccaatttgg atgcttcaaa gcttcgtttt
    11221 tagttatatt tgcaagttct ccctttttct ttcctctaaa tggaccacca gatgctgtaa
    11281 gtattatctt ttctatattt tttttatttt ctccatttag acattgaaat atagcactat
    11341 gttcactatc aacaggaaga atatttacat tgtatttttc tgcttctttc attacaagtt
    11401 caccagcagt taccaacgtt tctttattcg ctaatgctat gtctattccc ttctttatag
    11461 cacaaagagt tgggactaat cctatcatac ctactacagc agttaaaagg acatccactt
    11521 catcaagtga tgatattatt tttaatcctt ccattcctgc taaaacttct atttctatat
    11581 ttttaggcaa catttctttt aattttaatg ctttattttc ctcaaaaact gttacatatt
    11641 taggtttaaa ctctactatc tgttctagca ataattcaat attactgtta gctgatattg
    11701 caactatctc aaacttatct ctatgttctc taaccacatc caaagtttgt ttacctatag
    11761 aacctgtaga acctaaaatt gatatctttt tcatataata atcctttcta ataataaata
    11821 taccctgtca aaacattata acactactaa aacaattatt tttactattc atagaaattt
    11881 catatattat tccacagatt aaaattaatc tattattttt tgtattttgt ggaatattat
    11941 aaaatattct agtattatta aatgcccttt tttatttcca caacatcttc tattttacag
    12001 tctagtgcta aacatatctt ctccattgta tccatagaaa cagatttatc atttcctagt
    12061 ctgcctaaaa cagaaatact tatacctgat atatttacaa attccatttt ctttattttt
    12121 ctgtcaatta acaatttcca taatggagcg tatgaaatca tgttatcacc tcgcaaaata
    12181 tattctatca cattttatct attttggaaa attttcatct atttagataa ataattattt
    12241 atctaaatag atttcaatta tagtttatta aatataaata tgttaaaata aaacaaaagc
    12301 ataagttcaa aaattaaact ttatatatat ttatctcatt attttactaa aacataacta
    12361 tataaaaaat atttactatt atatatctat ttaaaacaca ttattatagt atcatataaa
    12421 catatgtaaa tatataatta aaaaagctaa agattagtaa atttataaaa attatatttt
    12481 gaataaaagg agaaagaatg ttgtatttat taaaaattaa tcaatttgaa agaagctatg
    12541 atataggaat ttttaaaaat gaacaatcaa tatataaatt tattgaaaaa ataccctttg
    12601 taaaaaaaga taatagtaat tttaattata caaattacta tatggaattt gaagatattc
    12661 ctgaatatta tgaagtaaat ttcaataatt atttatatat tattaccagg ttttcattca
    12721 ttccagatgg aactgagata ttttttactt gggaagaaat ccattgttgg gatgaaaaag
    12781 ttgctaccga gaaaactttt ataaatggcg aaacattagt ggattcatat aactttccaa
    12841 ataatgaagt gaaagactac atacaaaagc gagaatcact atatatagaa acaaaaaaat
    12901 attatgaaga aaaaggatta aaaattgaga gacatgcact cggttcagaa gatggtgaat
    12961 atgttgaact tacaaatgat tctatacttt atttactaga tatagaagca gttgaaataa
    13021 gagaacaatc aaaaaatata gaagaatttc tatataagta caaaaaacgt tttaaataat
    13081 ttatatttta aaatacataa caatacattg agtcatttaa cgaaatataa aatgactcaa
    13141 tgtatagact aataaaaata attatattat tttttagtta catatttgtt aaaaaattct
    13201 ttttcttcta tcggtttaga aaaataaaac ccttgtccta catcacattt aactgtattt
    13261 aaaaattcca agtctttttt agtttcaata ccttcacata caacagacat gcctatacga
    13321 tgtgacattt caataataca ttgaataata ctatatactt tctcccggtt aaaggtttct
    13381 ttaagtaagc ttttatctaa cttcaatcca ttaatagata gagaacataa aagacttaat
    13441 gaagagtatc ctgaaccaaa atcatccatt gaaattaaaa atcctaactt tttaagctcc
    13501 tctaaaatcg aaataactgg ttgagatatt tcattaaaag cactttcagt tacctctatc
    13561 tctatatact taaaaggaat ttcatattct ttaacagtat ctaaaaacct ttgataaaaa
    13621 ctattttggt aaatagtaac ccttgaaaaa ttaactgcaa tcggatatgt atattgattt
    13681 tttttcataa acgttttttt tacaaatgaa cacgcttttt ttaacatata aaagtccaat
    13741 tcaataataa atccactttt ttcaaacaat ggaataaatt catctggtga taaaaaacca
    13801 agttctggag aaaaccatct tacaagagct tctgcgctca caacatttaa agaagatatt
    13861 tgaaatttag gttgcaaata catatgaaat tcttcatttt ctatcgcttt atacatataa
    13921 ttataaatac tattttccat ttgtaactgt tttaacagat tttcattata ccatactgta
    13981 gaagaaattc ctcttgtttt acttaccttg tgtgcaatat ttgccttatc cattatagaa
    14041 ttgatagatt tataacctaa attatcaatt tcaactaagt aagctcctat acaataagat
    14101 atagtttgag ttacattcat atctaattca gagattatag cattagttag catttcccga
    14161 atatcctcta aaagagaatc tctatatttt gcaagtatta caaaattatc tgaacaaatt
    14221 cttgcacaag tttcttttat attaaaagtt ttagaaagaa cagttgcaac tattattaga
    14281 actttatctc catattcata tccatgtgta tcattaatat atttaaaatt gtcaatatca
    14341 atatttagca gtacatactc tttgttagat gcattgtgca aaatttcaat cgctctgttt
    14401 tcaaatgcaa cctgattttt tatttttgtt aaattatcag tataaaatat tcttttaaac
    14461 ctttttttat tataatattc ccaaattaaa agacttattg ttgagaatat aagataaata
    14521 aatagtgcag tagcaaaaga atacaatttt gcattagagt gtttttcaga tataaaaaca
    14581 atttgattag acaacacaga atactcttca ctcaatttat ataatttatc acctgaagca
    14641 ccatttctaa cttttataat ttctttcttc atagattccc acatagaatc catttgattt
    14701 agtttttgtt gaaattcttt attatcacat ctttgaaatc cattttctcc attacctgtt
    14761 cttagcttta tcagaatatt atccagtcgt tttatcaagt catcatttct ttcattattc
    14821 aattcttgct taacaacctt ttgagtttca ctgcgaatag taccagtgtg acttataatt
    14881 ttaatatgct ctatactgtg atatagagaa aatataaaaa gaataataat aactagccaa
    14941 atagcagaat aaattaaaaa atcatatttt ttcttcatta ttgttacctc atttgtaata
    15001 tatataattt gcaaataatc aatctcttat aaaaaatgat tatcaatatt ttacagtgat
    15061 atgtatttat cttaaatagt ttaatcattt ataccctata aattctacaa attcatcacc
    15121 tccaacacaa acagcaatat acgtttttct aaaatttctt tttaaacatt catcaacact
    15181 atcattaaat catttttaaa ttgctcaatt aatttactgc atcacttctt ttcttataaa
    15241 atatcacccc aataatatat ttgtataatg tctttatgtc tctttttaaa tttggcatat
    15301 acataatcta tattttaata aataattaaa atactattta cctacaattt aactctttga
    15361 atttatttta tcatattatg taaatatatt cctttttttt ggtatattac ttaaattata
    15421 tctatttttg tgcaataaga ccaataattt acttatctct tattaattat atttaaaaat
    15481 aaaatttata aatgtataat aaattagtta catttataca tatatattat aaata
//
LOCUS       DAGSWI010000097        15236 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.097, whole genome shotgun sequence.
ACCESSION   DAGSWI010000097 DAGSWI010000000
VERSION     DAGSWI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.097"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            60..1901
                     /locus_tag="MJF80_002729"
     CDS             60..1901
                     /locus_tag="MJF80_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HBZ0281379.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            2206..2913
                     /locus_tag="MJF80_002730"
     CDS             2206..2913
                     /locus_tag="MJF80_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281380.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            2907..4217
                     /locus_tag="MJF80_002731"
     CDS             2907..4217
                     /locus_tag="MJF80_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0281381.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            4334..5017
                     /locus_tag="MJF80_002732"
     CDS             4334..5017
                     /locus_tag="MJF80_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281382.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            5010..7397
                     /locus_tag="MJF80_002733"
     CDS             5010..7397
                     /locus_tag="MJF80_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBZ0281383.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            7677..8819
                     /locus_tag="MJF80_002734"
     CDS             7677..8819
                     /locus_tag="MJF80_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBZ0281384.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKKYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKVPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            8838..9362
                     /locus_tag="MJF80_002735"
     CDS             8838..9362
                     /locus_tag="MJF80_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBZ0281385.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIIFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKNCIK"
     gene            9462..10280
                     /locus_tag="MJF80_002736"
     CDS             9462..10280
                     /locus_tag="MJF80_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBZ0281386.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEVVSQLFAKKLEESLSPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            10358..10669
                     /locus_tag="MJF80_002737"
     CDS             10358..10669
                     /locus_tag="MJF80_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBZ0281387.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(10846..11529)
                     /locus_tag="MJF80_002738"
     CDS             complement(10846..11529)
                     /locus_tag="MJF80_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281388.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIHNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(11622..12263)
                     /locus_tag="MJF80_002739"
     CDS             complement(11622..12263)
                     /locus_tag="MJF80_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0281389.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            complement(12437..14440)
                     /locus_tag="MJF80_002740"
     CDS             complement(12437..14440)
                     /locus_tag="MJF80_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBZ0281390.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     NFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFNSTPPSSKMPIASRTAIVLLDNGI"
     gene            complement(14593..15231)
                     /gene="recX"
                     /locus_tag="MJF80_002741"
     CDS             complement(14593..15231)
                     /gene="recX"
                     /locus_tag="MJF80_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBZ0281391.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT         5885 a         1734 c         2450 g         5167 t
ORIGIN      
        1 ttatgtaaaa tataaatttt atattaaagc taaaactaat ttgaggaggc taatttatat
       61 tgaagattag aaggtattta attttggtaa ttataattct aattacaaca agtcagagtt
      121 ttatttctta tgggctagag cgatttgaag aaataaccat ttttcacact aatgatatac
      181 atggaagata tgctgaaggt gatgaccata tacaaatagg aaatctagcc acattgaaaa
      241 aagaaactcc aaactcaatt ttagtagatg caggagattg tttgcatgga cttccaatag
      301 taaatatgga tagagggaaa agtgcaattg aattaattaa atgtgctgga tatgactata
      361 ttactcctgg aaatcatgac tttaattatg gaaaagatag actgtttgag ttatctaaaa
      421 gtgctaattt aggagagaat actttaaaat ttttatcatc aaatattttt gaaaatggaa
      481 aaaaagtttt tgatagtaat gatataaaag aaatagatgg aataaaaata ggattttttg
      541 gactctcaac ccaggaaaca aagagcaaaa ctggatttaa aaatgtggaa ggattagagt
      601 ttaaaaatcc tatagaaagt gcaacagaac aagtaaatga attaaaagga aagggtgcca
      661 atgtaatagt tgcaatatgt catattggta caaatagttt aagtaagcca aatagtatag
      721 atatagcaaa tgaggttcaa ggaattgatt tgattattga tggacacagc cacactaagt
      781 ttgaaaatgg taaacaagtg ggtgatacat tgattgtaag tgcaggacaa tatttagaaa
      841 atattggcga gataaaacta actttggata ctaatgatgt agaaaatata aaaatagaaa
      901 ataaatcagt tagattaatt ggaaaagaag aagcattaaa gtataagctt gactccgctg
      961 taagtaaaaa ggttgatgaa ataaaagaac aacaagaaaa aatattagat aaagttatag
     1021 gggtaactac aaatactcta gatggttcat atgaaaatgt aaggacaaaa gaaactaatt
     1081 tgggtaattt aataagtgac attttactag ataaaactaa ggctgatatt tctttattta
     1141 atggagggaa tattcgagat acgatagaaa aaggcaatat aacaagaaga gatattgtag
     1201 atgtatttcc ttttagcaat actatagtta ccaaagaact tacaggagca cagattaaag
     1261 atgtattgga gcatggagta aaactttatc cagaaaaaag ttcagccttt ttgcaagtgg
     1321 gaggaatttc atattacttt aatcctaaac aaaaagaagg cgaaaaaata acaaatattc
     1381 aaaaagaagg aaagccatta gatttaaata aaaagtatgt agttgcaacg aatgattata
     1441 tagcatcagg tggagatgaa tttccttgtt tttcaaaaga gccagtacta aaggaatttg
     1501 gaaatttaga gagtgcagtc attgaatata tagagtacaa aaaagaaata tcaaaaaatg
     1561 tagatggaag aattggtatt aaatctaaag atgagttaat aagcaagaat catgagagtg
     1621 atttgatatt aagtgaaaat gaaaaaaata tcaatgataa tgaagagaat ctatctaata
     1681 atataaaaag ctctgatagt gataaaaata aaagcttaga taaaacaagt gctagagaaa
     1741 gttctaatct aggaatatct aaaagagaag ctaaagaaaa atctcctaaa acaggagatt
     1801 taggtttttc aaatagtata attatattta tagtttcaag tacattgata tgtttattga
     1861 attttaatca aaaagagtta aaggataaaa agtcaaaata aaaattaatg agcaaaaatt
     1921 agtagatagt aatttaattt tagataatag taaacagcaa aggaaattag ttatataaat
     1981 actaatttcc tttgttttat gttttaacat gattactttt aaataaaatt gtattttatg
     2041 aaaaataatt acactaaatg gcatagtatt atggggattt ttagttttta ttcaagtaaa
     2101 tgaaatcata ttaacaactt ctgatatatt tacaaattga taaaaaaatg attttattag
     2161 tatgatataa tttagctagt atatatgatg atggaggata tagggatgaa taatatatta
     2221 cttttggaag atgacaaatc tttaaataga ggaattagtt ttaaattaaa aaaagaagga
     2281 tacaatgttt ttagtgcatt tggtatagaa gaagcaaaaa atattttcgc aaaagaagag
     2341 atatgcttaa ttatttccga tataggtcta cctgatggta gtggttttga cttttgtgag
     2401 gagattagaa agaaaagtga tgtttacata ataatgttga cagctcttga tgaagaggtg
     2461 gatattgtca caggatatga tttaggtgca gatgattata tcacaaagcc ttttagcttg
     2521 atggtattaa tatcaaaagt gaatgcgctt atgaaaagag taaatacagt aaaaaattat
     2581 actctattag tttgtgatga tttattcttt tattatatag aaaataaatt aattataaga
     2641 acagatgata aggaagaaga gataatttta agtaagacag agacaaaact attaaaatat
     2701 ttgatggaaa attctatgca gacattgact aaagagcaac tgttagaatc tttatgggat
     2761 agtagtggta attttgtaga tgataatacg attgcagtaa atataagaag acttagacaa
     2821 aaggttgaaa aaaatccatc tgaacctaaa tatataaaaa cagtaagagg ggttggatat
     2881 atgtggggag aaaggagtat aaaaaaatgc taaaaaatat gaacctcaaa gatggtttaa
     2941 gtgtgaaaga tgattatata atgagaagag ttattgtata ttctgtttta tctgtactaa
     3001 tagtaaccct tgtatttata ctttttgaat atagcagatt acataaattg tatgaacatc
     3061 aaataaaaaa tactcaaaat attgctggaa tattgattga aaaatatcct aatgatgaaa
     3121 tagatatagt aaattctatt tataattctg attatagtaa ggtgtctact ggagaaaagg
     3181 catttaagaa atttggctat ggattggaaa ataaaatgtc aaatgataaa aattttagct
     3241 tatacttaaa atcttttttt aaggaatcat tttttatttt tttaatgatg tttatagtag
     3301 ttataggtat aatatattat tttataagat acataaataa aagattgagt aaaatttatt
     3361 ttatagtaga taagatgtca caagaaaact acttgaagga cactactgag tcagaagaaa
     3421 ctggttatct agtagaatat aatgaatact ttaaagaagg catatttagt aagataaata
     3481 actgtctata tgagcttaat agaagtttaa agataaagtt tattaaatta gaaaaagaaa
     3541 aagaaagtgt aaaatcatta gtaacagata tttcccatca attaaaaact cctttagcat
     3601 ccttaaaatt atacaataca ttacttatag aagaagaatt agaagaagat gaagccaacg
     3661 agttcttatt aaccaataag gcttctataa ataaattaga aaatttaata aattctttag
     3721 tgaacatatc aaggttagaa attagcatga taaacataaa aaaagaagat aatgatataa
     3781 aaaatactat tctaaatgca gtaaaaagtg ttacccctaa ggctaagagc aaaagtataa
     3841 cattaaattt aaatgaattt aacagtagaa ttataccaca tgataaaaag tggactgagg
     3901 agagtatttt taatgtatta gacaatgctg taaagtatac acaggtaaat ggagaaataa
     3961 atatatctgt agaagaaact gctaatttct ttaaaattat aatagaggat aatggcatag
     4021 ggattgaaaa aaatgagttt aataatatat ttaaacgttt ttatagaggt tctgctgatg
     4081 aaattgaatc tgtagaaggt agtggggtag gattgtattt aagtagaaag attttagaag
     4141 aacaaggtgg aaatattata gtttcttcaa aagtaggaat agggagcaag ttttctttgt
     4201 ttcttacaac tatgtaatat taaatgaaag gaaaatgtaa gcctctacat ttattatata
     4261 agtataaaca gctccagaaa taaaaaatta ataaaatatg gagtaatata acttagaatg
     4321 agaggataat tttatgaaaa tactatatac tgaaaattta tcaaagcatt atggaaaagg
     4381 agaaagctta gtaagagctt tggacaatgt tgacttagaa ataaatgaag gtgaatttgt
     4441 tgcaataata ggaaaatcag gttctggaaa aagtacactt ttgcatatga ttggaggtct
     4501 tgatatacct acatctggga aggtgtatat agataataaa aatatattta cattaaaaga
     4561 agaagaactt gctgtattta gaagaagaaa aatagggttt atatttcaaa gctataattt
     4621 aattccatct cttaatgtat gggaaaatgt agtgttgcca atcggtttag atggtagaga
     4681 agtagatgaa tcttttataa aagaactttt aaaatcatta ggacttgaaa ataaacacga
     4741 cgttttacca aatacattat caggtggtca acaacaaagg gtggcaatag caagagcatt
     4801 agctacaaga ccagctataa tacttgctga tgaaccaact ggaaacctag attctaaaac
     4861 gtctgatgag gttatgagta tattaaaatc tatgtctaag aagtacagtc aaactctagt
     4921 tatgataact catgatgaca gtatagccca aatggctgat agagttatat tcatagaaga
     4981 tggcagggtg tcaaaggttg gtgataaaaa tgattaatac tactaaaata gcaaaaagta
     5041 atttaaagca aaataaatca aaaagtattc taataataat tactataata ctttcaacct
     5101 taatgctttc ttctattgga atttatattg ttgatgcagg agcatatcaa aaagaaaaca
     5161 ctataaagta ttcaggtaat tatcaaggta tattagcaaa tgtagatgaa aaacaggctg
     5221 atatattgag taatcatgca gatgttgaat taactggtga aatgaatgga gtaggtgttg
     5281 aaaaactgga agatgattca aatatttctc tagcatacat gaatgaagat gctttgaaat
     5341 taaatagttt tgaatttatc aaaggaaaat tgccatcaaa agagaatgaa attgtgttag
     5401 atagtggagc tttaaaggca cttggttata aaaataaaga tttaggtgaa aagataaaaa
     5461 tatcttatga tgactataaa aatgataaaa aaattgaaaa agagttcatt ataagtggaa
     5521 tcttaaaaac tagcgaaata tctgaagcag gtaaatatta ttatgctatt atatcagaat
     5581 catatatgag aaatactaga gatatgtcac aagaagattt taatatttat gtgaaattta
     5641 atgataaaag taatttatca atagagcaag caaaagaaaa attagataaa attgctaatg
     5701 atataggact ggatactatt aatactgctg taaatgaaaa ttatataaat gctttaaaac
     5761 cagatatgga aacgattatg ggtggagttt ttgtagggtt agtaattgta ttatctagta
     5821 ttttagtaat atacaatata ttttatattt caatagttac aaaggttcaa gaatttggaa
     5881 aacttagagc aattggtgca actaagaaac aaataaaaaa tatagtgttt aaagaagggt
     5941 tcatattagc cggaatagca ataccaatag gaataatttt aggctatgta cttgcaaata
     6001 ttataataaa atcatttatg gatatagatg taaaatcatc acgattacca gttatattat
     6061 cggttgtggt aataagcttt atatctgttg ttttatcact tttaaaacca atgaaagtag
     6121 catcaaaagt atccattgtt gacgcagtaa gatatacagg aaataaaata agtaataaga
     6181 gtaagagaaa aggatataaa aatataaatc taaagagatt atcacatgcc aatttagaaa
     6241 gaaataaaaa acgtacttac atgacattgg catcacttat acttagtgga actatattta
     6301 taacagtatc aactgcctta gaaagttttg atgctgagaa aatggcaaga gaacactttc
     6361 cttatgatat agaagttaga ctatctggtt atgaaatgaa cagtgataaa aatcctaaag
     6421 ataatttgaa tattttacaa atggataatc ctctaagtaa agattttttt aatcaaataa
     6481 agaacatgga aggaattaaa agaatagaat ctgcaagaag tgtaaaaatt ggtatggagg
     6541 actatgatgt tgaatttaag tatgaccttt tacaaagtat aaatgaaaat gatgtaaaat
     6601 cattaaacaa gaatataata gatggaaaaa taaatcttga aagacttcaa actggagatg
     6661 aaatagtaat tactcatgtt gatacagcaa aagaaatggg agtaaaagca ggagataaaa
     6721 taagattaac tttatatgat ggagataaaa agataaaaaa agaatttaaa gttcaagcaa
     6781 tagctatggg ggttccaagt tttggtatag ggaaagattt tatagataga actttgaaat
     6841 acgattcaac tagtagcttg ggaatatata cagaagaagg aaagtatcaa gaaatcaaag
     6901 atagtatcaa gaagattgct aaaaataatg gatttttaga gactgacttt atagatagta
     6961 gaattgagtc aaataaggct actatttcat ttataaagat aatgggatat acgctaacag
     7021 gcataatagg tgtgattgga tttatgaatc ttgtcaacac aatgataact agcatagtta
     7081 ctagaaaaaa agaacttggt atgcttcaag ctattggtct tactaataaa caattagtga
     7141 aaatgcttaa ttcagaggca atgtattaca cttctggaat gatgatagga agtattctat
     7201 ttggaggtat attagggtat attgctgtta tatttctaaa aaagactgga ttatcttatg
     7261 caacatattc tttgccaata gtaccaatct tgttgatgat tgtgtgtatt cttatagctc
     7321 aatttataac aacttatttg ataggacgaa gttttaataa agaatctctt attgatagag
     7381 taagatacag tgaataaaat gcaaatattt atggttagtt taactagcaa aaaacatata
     7441 aaaaaatata gttaaagaaa ttcataatta aataggttaa agcatagtac agagcaaaca
     7501 atttaaacag atgtttgctc tgtattttat tatgtaatca ataatatatc taatatactg
     7561 atattattca tgttagaaaa tgtaccggta caattctaaa aaagtataaa attgtaccat
     7621 ataaaatgaa aattttgtat aataatcgta tagaaaataa ataggagctg atatatatga
     7681 ataagaagat taattatgca ccaggaccaa ctgaaactag ggaaaatgta agattagcaa
     7741 gagcagaaaa aacaacaaat ccagatatag atattgattt tgttgaattt tataaaaaga
     7801 catgcgagaa gtttggtaat ataattggaa caaaaaacga tgtttacata ttgagtggtg
     7861 aaggaatatt aggtcttgaa gcagcatgtg catcacttac agaaaaagga gatagagtac
     7921 ttgtaattga taatggtata tatggagaag gttttaaaga ttttgtaaca atgtatggtg
     7981 gggagtatgt gcttttttca agtgaatata ctaaaagtat agatattgat gaattgaaag
     8041 catttttaga tgaggatagt aattttaaat atgcaacagt agtgcattgt gatactccaa
     8101 ctggcgtatt aaatgatgta agcaaaatat gtccattact taaaaaatat ggaatattaa
     8161 ctgtagttga ttctgtagca ggtatggtag gagaaagatt aagtgtagat gaatctaaaa
     8221 tagatataat attaggtggt tcacaaaaag ctatttctgc accagcagga cttactatag
     8281 tcggaataag tcaagatgca aaaaattgta taaaaaatag aaaaactgat gtaataggtt
     8341 tttattgtaa tttaaatata tgggaaggct attatgaaaa aaaatacttc ccttatacaa
     8401 tgccaataag tgacataatg gggcttgata aagcacttga taatatatta gaagaaggaa
     8461 tagaaaatgt attaaataga catgaaaaga tagcttatag tgttagaaaa tctattgagg
     8521 aatatggtct agaactattt ttagaagaag gatattctaa tacagtcaca gctataaaag
     8581 ttcctgaaag tataggagct ttagaactta ctgattatat gcttaaaaat tataatacac
     8641 ttgttgcaac atcactaaat caatatatgg atactatttt aagaataggt catatgggag
     8701 agaatgcaaa tctaaataaa atagaacatg tactaaatgt tttggataaa agtttaagtg
     8761 cactaggatt caaaggaaat ggaaatttgt taaatttatt taataagtat tatttttaat
     8821 agataggggg atatagtatg gaagttacag ctaagcaaaa atttacaacg aaagacatgg
     8881 tggaaacctc actattaata gcattagtat ttattgcaac taaatttata aacataagat
     8941 taccaatatc tataaatggt gggcttgtgc atttaggaac tgcaatgttg ttcatatcag
     9001 caataatatt tggaggtaaa aaaggtgctt tatcaggggc tattggtatg tcactttttg
     9061 atttgatatc tgggtggact ttatgggcac cgtttacctt tatagttaga ggagttatgg
     9121 gttatttact tggaaagatt gcatgggcaa atggtaataa tgggaataat tttttaatca
     9181 atgtaatagg aatatgtgtc tcatcaattt ggatgctatt tggatattat gtaacagaag
     9241 ttatacttta tggtaatttt atagtacctc ttacttcaat accaggtaat ttaatgcaag
     9301 tattgatagg tcttataata gcgcttccta tttctaaggt attgaaaaac tgtataaaat
     9361 aaatataaag tgaactaaaa tttatgatag cataaattat actgtataat attaggatgg
     9421 agtacataat taaaaaattt tagtatagag gttagttcac aatgttatca gttacaagat
     9481 attcaatcaa aaatgttcgt ttaaagccgt gggtaaagtt tatttggtat tttgagacaa
     9541 aatcaaatat actgttaaat aataagttac ttccgacaga cagtatagat attattctca
     9601 atctttctga tgttatggaa tataaaattg aaaatcaaga ctatactgct agtaatatgc
     9661 attttaatgg aataagggat aaacatggat ttattattca acatggaaat atacgtgtta
     9721 tagggatttc tttttatcca tttggtttat atccattttt aaagatacca atttcagagt
     9781 ttaacagaca aattgttgac ttagaagtag tatcacagct atttgcaaaa aaattggaag
     9841 agtcattaag tccaacacaa tcagtagaaa aaattgtttt atatttagaa gaagtattat
     9901 tatccatatt agaagaagat ttaatttcaa ataaatatgt aaagcttcta aattcattta
     9961 tatatgttaa tagatatagc aatataaagg cattttgtga tgatacaaat ataaatatta
    10021 aaacattgga aagaatctgt ttaaaatata cagggtatac acctaagatt ttaaagagaa
    10081 tttacagatt taaaatggca agtaatcaac ttatttataa ttataaagat gatgaacttt
    10141 ttgattttat ttatgaaaat gagtactatg accaagcaca ctttataaaa gaatttaaaa
    10201 aattttcagg gacgtcacca attaaattta ttggtgagaa taagacgatt aaagaaaata
    10261 taaagtacag ctatttataa ttatactgtc gattttttac aatacctcca atttattttt
    10321 tgatatgata taggaaaata aattggaggt tttacatatg aaaattatta cagaaatagt
    10381 tgagtttaat gtggaagaaa atttatcaaa agaagagttt atagaaattg taaatgactt
    10441 agaattaaaa tttcattcta ttcaatctgg ttttattgat actgagcttt tatatgatga
    10501 aaagtctcaa aagtggatta tgatacaaca ttgggaatct atagaagacc tcaagagtgc
    10561 ttctagtaaa atgtttaaag attcttcaac tgagaaattt agaaaagctt taaagaatca
    10621 aactgttaag atgaatataa taccacaaat taaaagttgg aaattataga actgaatgta
    10681 aaagatagtt aaatagtatc aaactaaatt agccaaataa cattttgtat ttaaattgtt
    10741 atttggcttt tagtgtttat tttaatatga gtgttatata ctagaaaaac caacaaactg
    10801 ttagagtgtg gattatcata atgtcatatt cgggagctaa aagtcttaat actgatttgc
    10861 caattggtca atccatgcat gtccatctgg atatgctgtt attaaagcat ttacaagcca
    10921 atccctttgc tcttgtgata atagtgggac tgttacatcg aagtcttttt gataaccaag
    10981 cctatctatg tatattgatt tgcaaaacaa aaccacttcg ggagccatat atgggatatt
    11041 atctgatgtt atcagtctgg ccttatccat atctcttata attctttgat tttgggaaaa
    11101 aagaaatttt ccatctttct gtggattatg tatcaattca ataaaatcga actgtaactg
    11161 ttcgtcacac tgtatattca ttttatagtt atcatcctcg acttttttta attcaataaa
    11221 tgagcaatca ggcttaatcg caaaaacaca aatttgctct atatgttgct tgttaaggtc
    11281 gataataggt cgtaataaat aattacccaa gggctcatat atatcccatc cttgattaag
    11341 catatactga atgctctttt ctttatctgt gtcagaaatc catatgtcaa tatcactgtg
    11401 tgggcgaata tgcatattcg caaataattc tagggcatat ccaccacata ttgaatatga
    11461 aaagccacaa tcattaaaaa agtttacaca atctttaatt agtttgttca tagcaacctc
    11521 cttttgcatg tactgaaata aattaaattt ttaaaaatac caactaaatg tttgttttgt
    11581 gtaaatataa aactgccgat attaaggcag ttttatattt actatttaga aaatttatta
    11641 acaaattttt gatattcaca cattatctta tcattcataa aagaaatacc cataaaagca
    11701 gcttgctcac caaacaaagt aaaacgttct atcatctgtt gtggattacc tggataaact
    11761 gttggaatac accatgtgtt tagaagaaaa ccaaacattt gagcagcaat ctctggattt
    11821 ttaacttcca tagaattatc ttgatttcct tcttcaatca atgcttttat ccaaggcaca
    11881 atatcattta taatctcatc aaactgcgta gctaaatact taggattacg caatagtgat
    11941 gatgacatta aaaaaatgtc ttgtttattg tgttgttcat aagcatgaag caacctatat
    12001 tgtaattttt ctaatgcggt ctttccttca tcatttttta cctcataccc ataaaatcca
    12061 tcattataca accgacttgt gattgcctta attatttcgt cttttgattt aaagtgatga
    12121 taaatagcac ctcttgaaag gccatctaaa ttatctacaa tgtcttgaat cgttgtctgc
    12181 tcataccctt tttctaagaa taacttatgt gaaatatcaa gtattttttg tatagtatta
    12241 tcaggttctt tttttcttcc caaaaagctc actcctcaaa acaatcattt ggtatgttta
    12301 tcttagcaaa tacataatga tttgtcaatg tgtcttttta gcatgttgaa caggtatatt
    12361 gccatgcttt cattatttta tttttttgcc ttatactgga ctatatttgc agtttcctaa
    12421 tatatttggc tcatatttaa ataccattat ctaaaagcac tattgctgta cgagaggcaa
    12481 taggcatttt agaagaagga ggagttgagt taaagtatac atcataatct cttaagtttt
    12541 ctttttttgt gtttcctcca aactctacca tatcagtatt taatatcgtt ttgtagtttg
    12601 tattttgaaa ctttgaaata tcaactgatt gtattagatt agggtggaaa ttaaatataa
    12661 ataaatattt gcccttactg tacaccaaaa tctttttatc attatccata tgtataagtt
    12721 catgactatt ttggataaaa atatcactgt attttgtaag ttcgagcata gatttatcaa
    12781 aatttaataa ttgtttatac tttaagttgt tatcttctga aaggctccat tgtcttctgg
    12841 catacttata gctccaattg ttgccttctc gtggaaaatc aatccactca ggatgtccaa
    12901 attcatttcc cataaaattt agatatcctt cacctgctag agaaaaagtt attaatttaa
    12961 ttaatttaat aagagatatt gctctattga taacatggtt atttgagttt atttccatat
    13021 tccagtacat ttccttgtca gctagccaaa aaataattgt tttatcgcca actaatgctt
    13081 ggtcatggga ttcacaatat cctatatttt tttctccagg tcgtctagta gtaagttcat
    13141 accacatttt tcccaaatcc cagtcttcat cagataagtt agagatagtt tttatccaaa
    13201 aatcaggtac tcccattgca agtctatagt caaatccaat tccgccgtct tttataggga
    13261 tacacatacc aggcattcca ctcatatctt ctgcaatact tatcgaatta ggtttaattt
    13321 cttttattaa ttcatttgca aattggagat atgttatagc ttcaatatct gtattcatac
    13381 taaaatattt ttcataacta tcaaatgaaa ctccaagacc atggttatga tataacattg
    13441 aagttacacc atcaaaccta aagccatcaa aatggtactc atttaaccaa aattttatat
    13501 ttgaaagcaa aaaatgtatt acttcaggtt taccatagtt aaaaagtttt gtaccccaag
    13561 caggatgatt tccctttgaa cctgagtgga aaaattgatg ttcacttcca tcaaactcat
    13621 ttattccttc taaagtattc tttacagcat gagaatgaac taaatctaat aaaacagcaa
    13681 tccccattga atgagcagtg tttatgaggt tttttaagtc ttcaggagta ccaaacctag
    13741 atgagattgc ataaaagttt gaaacttggt atccaaaaga tgcataatat ggatgttcca
    13801 taatagccat aagctgaatg gtattgtatc cagctttttt tatcttagga agtatttttt
    13861 cagtaaattc attataagtt ccaatagatt cgctttcagt agacatacct atatgacatt
    13921 catagattag tggagaagtt atgtttttta agtcaaaatt attgtcagtc cagataaatg
    13981 gagtttttgg ttgccatatt tgtccattaa atccaccaaa atctttttga attactcttt
    14041 ttatatataa tggtatacgg tcaaatgttt ttccatttgc agtaacttgt acctttacct
    14101 ctgatttgtg aggtaagcta ttttgaccag gaataaatat ttcccaatta ccagacccaa
    14161 tttttcttag aggatgagat tttctattcc aattattaaa gtcaccaatt agtgacaaac
    14221 tatctgcatt tggagcccat tccctataga agaatccatc atttgtctga tgaaatccat
    14281 agtaaagatg agcatttgca aaagaactaa aatctttata gtttttcaat gtttttgatt
    14341 tcatatcctc ataatgtttc attcgctttt ttatatcaga ttcaaatggt tgtaggtata
    14401 catcaatatc taaaatttta aatctttttt gacttttcat tgtatttctc ctccataata
    14461 aatattacat ctaaaaaata atatttatct catactatct tctctgtata attgatatat
    14521 attatataat cctaataaaa agcttaagcc accaatgatt aatttcataa tttacctccc
    14581 tagtatttat acttatatat cgtattcatc aaaatttaaa agtttgttta aaactctttt
    14641 aataatatca taatcaaagc ctttatagga aagatgttga gaaatttttt gatatatttt
    14701 ctttttatct tctttcttta ctctttcata cctttttttt gctagataca tagcattttc
    14761 aaattcagtg tccttgtcta attcagatac tgcttcattg attgtacttc tattgatacc
    14821 tttattatat aaattctgct taattctgtt tttgccacat ttatttaggt taacatttgt
    14881 gttaacaatt ttttgagcta atacactgtc atttacgaga ttataacttt taagaaaatc
    14941 tatgaccctc tcaattatat tttcatcaaa ttctgatgat agtttttctt ttaatttttt
    15001 ttcagattgg tcagcatgag ataaaatatt taatgcttta tttttagctt ttatatacat
    15061 ttcatcatct aaaataggtt ttaattcttc ttcatttatt tcattcccct tttttaaatt
    15121 aaatgtataa acaagttctt taaatatagc aataaaaaat ttatcatcta cataaatatt
    15181 aactctatca tcatttcttt tttgttgttc tatttttgta attataccca taagtc
//
LOCUS       DAGSWI010000098        14777 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.098, whole genome shotgun sequence.
ACCESSION   DAGSWI010000098 DAGSWI010000000
VERSION     DAGSWI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.098"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            227..1402
                     /locus_tag="MJF80_002742"
     CDS             227..1402
                     /locus_tag="MJF80_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBZ0281392.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1575..2972)
                     /gene="asnS"
                     /locus_tag="MJF80_002743"
     CDS             complement(1575..2972)
                     /gene="asnS"
                     /locus_tag="MJF80_002743"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBZ0281393.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(3049..3282)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3451..3645)
                     /locus_tag="MJF80_002744"
     CDS             complement(3451..3645)
                     /locus_tag="MJF80_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBZ0281394.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3918..5591)
                     /locus_tag="MJF80_002745"
     CDS             complement(3918..5591)
                     /locus_tag="MJF80_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBZ0281395.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLEPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5601..8999)
                     /locus_tag="MJF80_002746"
     CDS             complement(5601..8999)
                     /locus_tag="MJF80_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBZ0281396.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(9166..10362)
                     /locus_tag="MJF80_002747"
     CDS             complement(9166..10362)
                     /locus_tag="MJF80_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBZ0281397.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSISYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(10383..11204)
                     /locus_tag="MJF80_002748"
     CDS             complement(10383..11204)
                     /locus_tag="MJF80_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBZ0281398.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            11668..12762
                     /locus_tag="MJF80_002749"
     CDS             11668..12762
                     /locus_tag="MJF80_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBZ0281399.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
     gene            12913..13269
                     /locus_tag="MJF80_002750"
     CDS             12913..13269
                     /locus_tag="MJF80_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281400.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTFEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(13362..14630)
                     /gene="eam"
                     /locus_tag="MJF80_002751"
     CDS             complement(13362..14630)
                     /gene="eam"
                     /locus_tag="MJF80_002751"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBZ0281401.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDTCDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
BASE COUNT         4726 a         2553 c         1756 g         5742 t
ORIGIN      
        1 tattctattc tttattttca gtagtaaact atctgtaaat cttaattata ataaagtatt
       61 aatcaataaa gttactcgac attatctaat aaattttatc tatcacaatt ttagattgtt
      121 acacacaata tattttgata taattaaatc ataaacgaag tattccaaat atctaatgat
      181 agaaaggaat acttattttt atgctttatt tttaatggag gcttatatga acttagaaaa
      241 aataaaaaaa ttttttggat ttcctctaga caaaaaacac aaagacaatg ttgagaaaga
      301 actctgcaat tatctttatc atacgggtaa aatcatttgc ataataatag taatatctca
      361 aataattatg atgatatcaa tctcagctag agaaggaggc ccttttgtca caccaagacg
      421 ccaatggtat ttttatatgt atgtaacact attttgtgta tcttctgtct ttatgtttgt
      481 attttcttac ctttggaata aaaaaaagga ttgctgtacg ttttatataa atttatggat
      541 aatttatgtg acttttttct gcctatgggg ttgtgtaatt actctacttg accagttagg
      601 tggaaatgac ctaagtgtat ttagttatat tatgttagca gctgcagctt tctcagtatt
      661 aaaatttgga gaatcagtaa tagtttttgg aggatgcttt ttgtttctta atttattctt
      721 acctgggatt caaactatac caggcaatga gtttagtaat attgtcaaca cattttttat
      781 aacatccctt gccatttttg tttctacaat gctatataga aatcgtgtat ttacttacta
      841 tgataaactt ttaattagtc aacaatatga agaaatatct catataaata atttacttaa
      901 cgacaaagtt atgacagatg accttactaa aatgaacaac cgtaggtacc ttgaagatat
      961 tctacaaaaa aaattaatca gccatgcaaa tcaagaacct ataactggaa tgatgataga
     1021 tatagattat tttaaacaat ttaatgataa ttatggacat caaaagggag actattgtct
     1081 acaaaatatt gccaaagtaa ttttagattt tacaagttct gaaaatgaac atgctatacg
     1141 atatggtggt gaggaatttt ttatatgtat ttttaactgt aatgaggcag atgctaaact
     1201 taaatcagaa tgtttacgaa aaattataga gtcctgcaat tttgagcgtg atgatgttcc
     1261 tttaaaatgt gtaactgtta gtattggagt ttatacaaag aaaattggaa agacaataac
     1321 attaaatgag tttataagta attgtgataa ggccctatat actgcaaaag aacaaggacg
     1381 taattgtgtt gcattttatt aaaaggttac atactattac ataaacctat aagtattata
     1441 tcgcaaaata aaagtaatca aattatatat ttatagtaca ataaaaataa cttgcatatc
     1501 tctaaaatag ataaatatgc aagtcatttt agtgtttata cttaaagtta tataagtttt
     1561 attatatata attattaaaa ttcagcattt ttaggagttc ttgggaaagg tataacgtct
     1621 cttatgttag acatacctgt catatacata attattcttt caaatccaag accaaatcca
     1681 gagtgagtag cagtaccaaa ctttctaagt tctaaatacc accaataatc ttcttctctt
     1741 aatcccattt cattaattct atctaacaat acatcttctc tttcttctct ttgagaacct
     1801 cctacgattt ctccaactcc aggaactagt aaatccattg ctctaacagt tttaccatct
     1861 tcattcattc tcatatagaa agcttttata tcttttggat aatctgtaac aaacactgga
     1921 caattatata tttgctccgt tatatatctt tcatgctctg tttgaaggtc acatccccac
     1981 tcaacaggat actcaaattc atgcccagat tcaattaata attccactgc tttagtatac
     2041 gttattctaa taaactcaga gtttactatc ttatttaatc tttctaacaa tcctttatct
     2101 ataaaactat taaaaaattc catttcttca ggagcatttt ctaatacata gcttataaca
     2161 tattttatca tatcttctgc aagttccatg ttatcttcaa ggtctgcaaa aactatttct
     2221 ggctctatca tccaaaattc tgaagcatgt cttcctgtat aagagttttc agctctaaat
     2281 gtaggtccaa aagtataaac gtttctaaaa gaaagtgcca ttatctcggc atttaattga
     2341 ccactaactg ttaaattagc ctcttttcca aagaaatctt gagtatagtc tattttgcca
     2401 tcttcagttt ttggaatatc atttaaatcc atagttgtta ttctaaacat ctctcctgca
     2461 ccttcacagt cactacctgt tatgattgga gaatgtgcat atacaaagtt tctttcttgg
     2521 aagaatttgt gtattgcata ggcagctatt gatcttactc taaatacagc tgaaaaagta
     2581 ttacttcttg gtcttaaatg tgctatcgtt cttaaatatt caagcgtatg tctcttcttt
     2641 tgtagtggat acgaactatc tgattcccct tctacaacta tgctttttgc atgtatttcg
     2701 actggttgtt tagccccttc tgtagatact aactctccct ctacaagtat agatgagctt
     2761 attgctaatt ttcctacctc tttaaaattt tctactttct catcaaatac tatctgcata
     2821 tttttaaaga agcttccatc atttagctct ataaatccaa aatttttaga tactctagat
     2881 gttcttatcc atccagctac ttttacagtc ttccctatgt actgttcttt ttctctgtac
     2941 aattctttta ctgttataaa ttccttttgc attttaaatc ctcctagtat aattgttatt
     3001 aatttctttt aaatataatt tcaataaaaa aagcccttca tccctatata aagggacgaa
     3061 agactattac tctcgcggta ccaccctagt tgtcataatg acctcttagt taagcaatgc
     3121 ttaaagccta taacggtgcc ttccgtctaa gtctacttac taaataagct ttcgattaga
     3181 aactccaaga tgttcttcaa tataaacctc gtattgagct ttcaccatcc tcaactcgct
     3241 agaactacat tttatatcta ctcttctctt cacagtattt atcttttatt atttctgcta
     3301 attaacaaaa ttataccaat ttataaattt ttagtcaata aatttatata attgatactg
     3361 attgttattt aaaatataat aattctacct tacataatga taataaatac ttatcttcat
     3421 tacttcttta ttacttcaat ttccaaaatt ttataattta cttccattgt caacccagtc
     3481 ttttgcttct tcgacaccct taattgaagg tattgaaact ttactatgag gttttaattt
     3541 ttcaacatca gcccatgctg ctgtcgactc atttccaact aaaagtgaac ttttaagtct
     3601 tttattatct ttacctttta atgctcttgc ctttttactt tccaaaacta tcacctctat
     3661 tatttatttt gtaatagttt tagcaatttt acaaataaaa ttcataagtc taaattataa
     3721 ttatttttaa attaacataa tatcttttca atacttaatt tatacctatt tatatacaaa
     3781 atatttactt tgtgtattca aaatataaaa ctgtttttta attttcatat ttatttttta
     3841 tgctattttt ctactctatc taaagtattt gatacaatta aactaataat caattataat
     3901 tttacatagg ttcttatcta tagagtattt gctatctgtt gaatcgtatt ttttaaattt
     3961 aaaatcccat atccctgcga tacatttgga tatgtatata tctcaagttt tgtagcaccc
     4021 aatattaaat atgtttttaa aacttgtgta aacaatgata atctcggtac attatcctgt
     4081 ttttctaagt attccatcaa taaggctaaa acacctgtga ctatagaact gctcactcct
     4141 gttcctgttc ctgtattata tgttccattt ttatatgtgg atattatatc tacacctgaa
     4201 gctactatat ctggcttaat tcctcttcct ctaattggtc ctttcgaaga accaatccac
     4261 atactatcag tcttattgtt aaatgtacca actgtaatta catcatcact agcagcatac
     4321 atagttatag tagctactga atcaggctct aaaaaccttg tgtctggagc tatcaaattt
     4381 ttatttggca agtatatgtc atattctcca tttattataa gttctgaagt caatcttaaa
     4441 atccatactc caggttttat atctcttaat cttatctcca aattttcttt tccagaggta
     4501 atatatggat aaataaatct cattgcataa gtagtatttt ctaagttaaa ttttcctcta
     4561 tatatataaa agtctggaga ataccttata tcatgactaa cttcacctga aggagatatt
     4621 atctgtgctc ccactttatc tggaccattc gtatttagtg ttatatcaag agcatagtca
     4681 tctccatctt gtattattac atcttgaact tctcccacac tgctaaatct gccagaataa
     4741 tgaatatctg tattcccttg atttcctgca ccactcacta ctaccacacc agcactactc
     4801 aatatattaa aagtatctag tattgaagta gttgctacag cacttgactt aactccaata
     4861 gtcaaattta ttatcagtgg tttattttct gttcttgcta tgttagtaac atacgttata
     4921 gctgctaaaa aatctgaaac actgtaattt atccttccag cataataagt atctgtatat
     4981 gattttaatt taactactat taagtctgat tctgtagtaa tacctctgta ctgagaattt
     5041 atccttccat ttccaactaa tataccagaa actaaagttc cagtacctat attatcttga
     5101 ctcaaactcc catcatttct atttatggct atatttaact cacttcttgt aaattcactt
     5161 ccaaaaataa aaccttctgg aggtggattt gtatttgctt cttggtccca taaatataaa
     5221 actttactag ttccatcatc atttataaaa tcaggatgca aataatctat cccagaatct
     5281 ataactgcta ataaaattcc tctccctgtt atatcattat atggattttc ataaatataa
     5341 tcagtttcag ctgctgttgt aatggtttct ccattattta cattattagt tatttcaatt
     5401 agactgctca ttggttcaga ttcttcccac caagcaactt gtattatatt atttaaaata
     5461 gtttcatcaa aatctaaagg tacatatatt actgcaagct gactatttaa aaccatatac
     5521 ctatttattc cattttcttg tagggcagat tctatatcac cttgataaat tatacaataa
     5581 gatttttcca taaatccctc ctatcttaaa acatcaaaca ttcctctaac atccaagagc
     5641 ccatatccag aatttgtatt tggatatact gtgtttgaat cttttctagc accagcttgc
     5701 ataaaagttt ttattttttg cacatatgct tggtttggat atctaccgtc aacaaaagta
     5761 tattgaaaat acattgcagc agcaccagct gcatgtgcac ttgctgctgc tgtaccagtt
     5821 atagttgcat acgtgttccc aggataagca gctattatat tgacacctgg tgctactata
     5881 tctggtttta acctgtcttc aatagttggc cctcttgatg aactttgcca taaactacca
     5941 ttaatggtat tgtaggctcc tatggttatt aaatcatctt gaatcgcagg ataatttata
     6001 gtgtaaaaag ggtctacctc tctaaatcta gttccacttt ttaaaagttc tctatttgga
     6061 aggtataagt tatatcttcc tgttataata taaactccta ctaatcgaat cttccataca
     6121 cctctttttg cattctttaa agtaacatta gtaaattgct gtccagaaaa agttgttggg
     6181 tatatatatg ttatagaata ttcagtcccc tctaaatcaa ataaacctgt tactttatta
     6241 taatttgaaa ttccaacact tttactttct tcaccagtgg gagatactat tattacatca
     6301 gctttatctg gtctatttaa ccaaagttct aatgataatt cttcctcgtc ttcatttaac
     6361 tctaattcaa cttcaacaga acctccaaca tatggtatta ttccagatgt atgtgtctgt
     6421 gtattgccct catttcctgc tcctgccgtt atacataacc ctctagtaaa gaacgctttt
     6481 tcactattac tcctattggt taaacctgct aggctactgg tacctaaaga catatttata
     6541 actaatggtc ttccaagttc aaaagctttt ttatatgcat actgagatgc agcaaacaac
     6601 attgcactat tataaaaatc atctatcttg ccgagtttta ttattattag ctcagaatct
     6661 tctgctatac ctgcatattc actatttaca tttccaagcc ctgcacatat cccactcaac
     6721 atagttcctt gaccaacttc atcctgagat agactagggt cattttctgc gatagctcga
     6781 tttatatctt ctcgtgtata ttctgtccca atataaaaac catcaggagg agttccttct
     6841 tttgtttggt cccataaata tactattttt gaagtgccat ctggataaat aaaatctggg
     6901 tgtagatagt ctattcctgt atctgcaatt gatattagag tccctcttcc tgttatatta
     6961 atattaggat tatttttaaa gaaatttacg cccatttctt ctgttgcaac tacacctcca
     7021 aaagtacctc gattaatttc tccaagtaat gtcatctttg tagtcgatac agttcttatg
     7081 attgaaggaa gttgcaaaac tcgttgtatg ctattatagt ctgtattatt tatagatagt
     7141 acacctaatg aatcacttat cttaaaaaac ctttgactca tacctaaccc agctagttct
     7201 tcttcaaatt caggagtatg gactatatct attcctgata gtattcctga ttgtctactt
     7261 gcatttttac ttatataaat ttcattctct aagtcattat gtatttgcat attataacca
     7321 tctataaact tataactatt ttcctcatct ttaaatgact tattaatact tctatatcca
     7381 atatcctctg tttctaaatc ttgatttata ttagatagag tgtacaaatc tatatctgat
     7441 aaatttaaaa aaccaaatcc tgatgaatta tttggataag attgattact taatctcctt
     7501 gctcccttta gaagtaatgc ccttaatttc tgtgaataca aaaataaatc atttccattt
     7561 acaattcccc attccataaa caatgaacaa acccctgtaa catgaggtgt agccatgcta
     7621 gttccagtta atgctccact tgtacctcct ggtaaaaaag atactatatc ttcacctgga
     7681 gctaataaat caggtttaaa aacacctaat tgtgtatctc cttcaccaga aaatatagat
     7741 actatatctg ttcttgaatt aaaactacct actgttataa ctctacttgc tgttccagga
     7801 actgttacag taagctcttg agtaggaatc agaaatctag tatttctatt tagaccttca
     7861 gaagttggca aatatatatt tacattacca gttactatat ctattggttc aaatacaatt
     7921 ttccataacc caggagttat ctgtgtattt gaacttaatt gtagagtaac tcttcttgtg
     7981 agagaatacg gtgctatagg gtaaaaataa ccagttattc tagtttcacc caaagtattc
     8041 cttatctcac ctgaagttag agagattgct tgtgtttgat tatttgatgg gtttaccaaa
     8101 tgtacactaa aatcatcaac aaaatctggc catatattta tgtttaatat acgctctcct
     8161 tccccaacta taaactcaac ttcttctgta atattatttt gaagcctaat ccttttatga
     8221 cctcctttat ctgcattatt tccagctgct acaactatat tgtttttcca aaataaacac
     8281 atatcatcta tatattgttc aaataaagat gttcctctat gtgaaccttc attacttcca
     8341 taacttatat ttaatgtaac aggcattctt aattctaatg ccctatctaa aataaattta
     8401 attgccctca taaactctgt acttctcgaa aaaatatcgg tctgtatatt tccaactcta
     8461 accactatta ttctggcatc acttgcaata gttgcacaaa tacctgcaac atgtgttcca
     8521 tgtaagctag ttgttgaaat aggtatatac atacttccat ctattgcatt atttatatct
     8581 tcattagtat ataatgttcc ttctctaaaa ccttctggtg gatttccttg aatagattgg
     8641 tcccaataat ataatatctt agaccttcca tcactatctc taaacacagg tagagtataa
     8701 tctattcccg aatcaattat accaattata gttccttttc cagttaaacc agttctattt
     8761 ttaaatcctg ttattcctgt acttgaaaaa ctttgcacat cctgagtctg taatataaaa
     8821 ggcttttcta taaattcaat ttctggataa gttaaaagta tatttacatc ttcttcatta
     8881 cttgatgtta ttattgcata tgatgaatta agtatctcta ctgatacacc taattcttct
     8941 tctaacctca atatatctcc attgtatttt acaattaatt cataatttat tataatcacc
     9001 tcgacttcta aaattattat taatgtatat gttttgtgtg agtctaaaat gtattagact
     9061 atataatttt ttaagtttca gttatacaat ttataataga tatattaaaa aaataaataa
     9121 tgatggtaca attttgaata aatcatacca tcatttatat ttactttatc ttgaaaccat
     9181 taaatttttc aatgctttct caagaccttt tacagtcaat ggaaacatat taaattcact
     9241 ttgtatcatt ctttgagttt gataccaata actaattgaa tcccatccat ctctaagttc
     9301 tggattcatc catacaattt tactgtactt tctcttaaac cttttaagcc actctatacc
     9361 aggtgtataa ttatatggac cacgatagtt accaccaata tgaagcaact cagaaggcga
     9421 catagaagca tctccaacaa ttataacttt atagttttta tctatatttt ttaatatcca
     9481 ttcagtatta atagatttac ttaaccaaca ttcaggtgtt gtaaataatc tatcataaat
     9541 acagttatgg aaataatata tctttacatc tttaaaatga tttgatttac ttacagactg
     9601 aaataatgta ttacataaag tgctataccc tctcattgaa ccacctgaat ccataagcaa
     9661 taaaagcttc actgtatttt ttcttggctt ctcaaaaaat aactttaaat atcctgcatt
     9721 attacaagtc tcttcaatag tcttatccaa atcaagctct gtctttggag catctattct
     9781 agtagaaaat tgacgaagtc tctttagtgc aacttgaaat tgacgcatat ttaaaacttc
     9841 atcatctcta aaatctctgt attttctttc tcctgcaact tcaagaacag ctctaccata
     9901 agtagaattt ccacctatac gtataccagt ttgccctttt cctgaatgtc ctaactctga
     9961 agtgcctcct gtgcctatcc aataattacc accattatga ggagaatctt gttcttttat
    10021 tcgttcctcc aatttagctc ttaatttatc aagatttata tttggatttt gttctaatct
    10081 ttcaaactct tctaaatcta tatctggttt tttaagccaa ttcataatct cttctggaat
    10141 ttcttcttgg tgttctatac ctttaaaata ctccaaaaac actgcatcaa acttatcaaa
    10201 atcactttca ctttttatta aaatcatcct gcaaagatag taaaaatttg aaaagtttga
    10261 atagcacagt cctttatcca atgcttcaat taaagtcatc cattcattta aagatacatt
    10321 taatcctctt gacctcaata aataaaaaaa ttcaataaac ataattatcc agctcctatt
    10381 aactataagt gtctttgcat agattcaata tcctcattat tcttaagtag tattcctgca
    10441 aaaggaactt ctttttctat tttttcaata ggcattccac tcaaagttaa tgcttgtatc
    10501 cagtctatta actctgatgt actaggcttt ttttgtatgt cctttaaact tctaatccaa
    10561 taaaaagtag tcattacttg ttccaatagg tgttcttcta ctttatcaaa atgtacttta
    10621 actatttctt ccatcatgtc tctatcagga aattctatat aatgaaatat gcatcttctt
    10681 aaaaaagcat ctggaagttc tttttccgca tttgaagtta taataactat tggtctttgt
    10741 ttagctctta cagtttcttt tgtctcattt atgtaaaact ccattttatc caattcccaa
    10801 agcaagtcat ttggaaattc taaatccgcc ttatctattt catctattaa aagaattact
    10861 tgttgatttg agctaaaggc ctctcctagt ttaccatatt ttatgtactt gctaatatca
    10921 tctacacctt ctcctccaaa ttggctatca taaagtcttt gaacagtatc atatacataa
    10981 agcccttctt gtgcttttgt agttgatttt atattccaaa ttactaaatc tttttttaat
    11041 tcatttgaaa ttgcttgtgc tagcatagtt ttacctgttc caggttcgcc cttaatcaaa
    11101 agtggctttt caagagcaat agcaacatta actgatgcca tcaattctgg tgatactaca
    11161 taatcactac ttcctctaaa gttagatatc tcttttttta tcataaatcc tactcctttt
    11221 aaacagtatt ttctaaatgt atatagactt tattactatt tttgatataa aaataataat
    11281 acaatacatt tgtatagtat tattctaaaa ttctatatct aaataaaatc acctctaaaa
    11341 tatttaattt ttatctttat ttgtcataat taaatgaaat atatatttta aagaataata
    11401 aaagatagct tattgtacaa aatttaaatt ataaaaactt agtttaatca aaggcaccac
    11461 tttttttcat tttagattat atataaacac aatataaata atttttatta ttttagctat
    11521 aaacataaag ttttaacgta tttataattt catattaatc atactatatt aatactgctt
    11581 tttattgtat aataagctat aaatattaac tttaacttag atttaagtat atctaaattt
    11641 aataaatata agaaaggttg tgttattttg caagataaaa aaatttatct aaatattgta
    11701 ctcactgtag ttgtttcata tatcttgatt aaagttattg ataattacaa atattttttt
    11761 ggagtgataa gtttattaat gtcactacta acaccattta taatagcatt tgtattagca
    11821 tatatattca acccactagt aaagttctta gaaagtaaat taaattttaa gagaatatat
    11881 tcgttacttc ttacttatgg tgtactcata gttatactaa tttcgtttat attatttaca
    11941 gttccatcca tagtaaatag tttagctgat ttagttgctc aaattcctac atacatagat
    12001 aaaacagagc agtttttatt cgacttaggc aagagtttac aatcagttga ccctaataca
    12061 ttaaaagagt atggtgacaa aattatgagt gtaatgccta aatttagtaa cttgcttatt
    12121 ggttcacttg gtgggatatt tagcactaca ttttcagttg gaaaatttat agtacaattt
    12181 cttttaggtt ttataatttg tttctatata ttattagaaa aagaaaaatt cttactgttt
    12241 acaaaaaaag tagtttatat ttccttaggt aaaaaatatg gagactttat aatagaacta
    12301 tgtcaaagct taaatttaaa tattggtaaa tactttacag gtaagatttt agattctttt
    12361 atagttggag tactttctgg aattggtctt tactttttaa aatctgaata tgcactatta
    12421 tttggtacac ttatgggtgt catgaatatg gttccatatt ttggtccagt aataggtatg
    12481 gcaccagttg taataattaa tttattctcc aatccaacaa ttgctttaac ttctttaatt
    12541 tacttaataa taattcaaca agttgaagtt acatttatag agccaaaaat cgtaggtgga
    12601 caactaggtc ttagcccatt ctttacaatc ctagcagtta ctgttggtgg aggattcttt
    12661 ggtatacctg gtatgattct atctgctcct gtgatgggcg ttataaagat atacttctgt
    12721 gagtttgtaa atcgtagaca tgataaaata caaatggaat aaaaatacaa tttaattacg
    12781 attttgtaat caaaagttac agctgtgtaa ctgtaatatt attctcttta atggtaatat
    12841 ttaattatag gaaacataca atattattaa gtaatgaatc ttatataatt aaacatgaaa
    12901 gagggtttta gtatgaaaaa agcacttata tctttattag ttttatcttg tttgttatta
    12961 actgcaccag taaatgaatc ttcttatgca gattctcaaa agaatgttcc tgtaagccaa
    13021 aatactaaat tgactcaaga acaagctaaa gaacttttag taaaatacaa caatgaagtt
    13081 gattacatat atcaaggaaa tgctagtgat tttgaagcat taaaagctaa aaatcttaat
    13141 ggttatgtat ttttaccaga tgcagatggt gatattggtt actttgtaaa tgaaaatact
    13201 tttgagatat attacttcca cccaagtgga tatcttgaat tagttaatca acaacaaaat
    13261 aataaataac taataaaaaa tggatgcaaa ttgatataaa tttgacatcc attttctttt
    13321 tattttaaag ttgtttcttt ttataaactt taaatacttt tttaatcctg cgcttgttct
    13381 tttataagtt tctttatatc tacagcaggt tcatcttcca tttttataac ttttccttcc
    13441 catgttctaa gcataacgta atctgttcct tttgatacaa ggtattgagg aagcaaagga
    13501 gtctttccgc ctccttttgg agcatttact atatatgttg gtatagccat tcctgatgta
    13561 tatcctctta aatactccat gatttcaaga ccatcatcta ctgatgtatt gaaatgtttt
    13621 gttcccttta catgtttact ttggaatata taataaggtt ttactcttat tttcagtaat
    13681 tcttgattta aacatctcat tacaaattta tcattattta ttccatttaa taatactgcc
    13741 tgatttccta atggaactcc tgcatttgct aacttttcac aagcttcttt agattcctta
    13801 gttatttcca ttggatgatt aaaatgagta tttatatata cagggtgata tttttttagc
    13861 atattgcaaa attcatctgt gattctttgt ggcattgtaa caagagtcct agtacctaat
    13921 ctaacataat caacatgtgg tatctcttta agttggctaa gaatccactc taaataatca
    13981 tcttttaatg taagagcatc tccaccagtt actagtacat ctcttatttc ttcattttct
    14041 ctgatatagt ctatagattc ttggataata gcttttgact tatgagaatc ttgttgtcca
    14101 atatttcttc ttctctgaca gtgtctacaa tacatagcac actcatttgt tacatttatt
    14161 attaatctat ccgggtatct tctagttata catcctgctg ggtttgtata ctcttctccc
    14221 attgggtcca aatcttcttg ttcatcttcc aactctatat gtgttggtat agacagtaat
    14281 tttattgggt cacatgtatc ttctgggtct ataagactca aataatatgg agatattgcc
    14341 catctaaatt gagtaccaac ctcttttata tattcttttt cttttttagt tagagttata
    14401 atttttgata aagtatctac atctgttatt ctatttgata attgccattt gtagttattc
    14461 caatcctctt cagttccatt taagatactt aatattttct gttttcgttt atttatctct
    14521 tcttcttttt caagacctct gttaatcgtt tttctagcct gaatataatc atcaattttt
    14581 gattttaatt cagcagctct ctctaaggat attctagttt gttcattcat aattttttgc
    14641 tatcaaagtt aactacttgt attatttgat agcgtcctcc tttctggtgt gtttatatca
    14701 ttctgatagg cagatttaat tttgccatat agattttata acaaatgaaa ttgaattaat
    14761 ttttgcattt tttctgt
//
LOCUS       DAGSWI010000099        14614 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.099, whole genome shotgun sequence.
ACCESSION   DAGSWI010000099 DAGSWI010000000
VERSION     DAGSWI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.099"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            184..1104
                     /locus_tag="MJF80_002752"
     CDS             184..1104
                     /locus_tag="MJF80_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBZ0281402.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILHIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            1219..2376
                     /locus_tag="MJF80_002753"
     CDS             1219..2376
                     /locus_tag="MJF80_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBZ0281403.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(2461..3960)
                     /locus_tag="MJF80_002754"
     CDS             complement(2461..3960)
                     /locus_tag="MJF80_002754"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBZ0281404.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            4556..5980
                     /locus_tag="MJF80_002755"
     CDS             4556..5980
                     /locus_tag="MJF80_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBZ0281405.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            6015..8144
                     /locus_tag="MJF80_002756"
     CDS             6015..8144
                     /locus_tag="MJF80_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBZ0281406.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIRK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            8174..8596
                     /locus_tag="MJF80_002757"
     CDS             8174..8596
                     /locus_tag="MJF80_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0281407.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            8615..9766
                     /locus_tag="MJF80_002758"
     CDS             8615..9766
                     /locus_tag="MJF80_002758"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBZ0281408.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEDILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      9872..9973
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10156..10728
                     /locus_tag="MJF80_002759"
     CDS             10156..10728
                     /locus_tag="MJF80_002759"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBZ0281409.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            10792..11442
                     /gene="fsa"
                     /locus_tag="MJF80_002760"
     CDS             10792..11442
                     /gene="fsa"
                     /locus_tag="MJF80_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBZ0281410.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            11594..14518
                     /locus_tag="MJF80_002761"
     CDS             11594..14518
                     /locus_tag="MJF80_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0281411.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGLGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         5922 a         1581 c         2587 g         4524 t
ORIGIN      
        1 ttatttttat atggagttaa atataagtat atttttaatt taaaatatgc ttatatttaa
       61 gtctattact ttatttgaaa caatttgtag aatttcaaat tccttgacag taataagtaa
      121 gtaattgtat aatcaaactg caatatatta ataatattag tgaaaatgaa aggattataa
      181 aatatgtata aatcagaaaa tttttcaaaa aaatctgtat taataaattc atttttaagt
      241 aaatttttaa aaataattgg atggggtttg gtaagcttat ttacattatc agccttagta
      301 ctttcaacta tttccattat aattggatgg gctgtaatac ttcatattca agtatgtgca
      361 gtatttttac ttataggagg agtttgtatt tttaaaggta agagattagg agaccaaatt
      421 tctcgttata aaaaatattg tacaattata aacaatagaa atattattcc agtagaactt
      481 attgcagaaa acacatctaa atcattaaat tttataataa aagatgtaca aaagatgata
      541 gacaaaaact actttataaa tacttatatt gataaaagaa ataatcagat tgtgttaaca
      601 aatgaagatt ttgttccacc aagatatgaa gatgtagcat atgaagtcaa agaagaagaa
      661 aagactgatg aatccaatga agttgataca attataaata aaggaatgaa ttatctaaca
      721 caaataaaag aagctaataa aaatattaaa tcagaatcaa tgggcgataa aattgtacaa
      781 gtagaagatg ttacaagtaa aatatttgat gttgtaaaac atgacccatc aaaacttact
      841 caaatacaaa aatttatgga ttattatctt ccaaccactt taaaactatt aaattcatat
      901 catacattgg aagaacaagg aattgataga gaaaatataa caacaactat ggaaagtatt
      961 gaaaatacaa tgaatacgat tgtagtagct tttgaaaatc aattggacta tctgtttgaa
     1021 gatgaagcca ttgatatttc tacagatatt actgttttag agaatatgtt agtccaagaa
     1081 ggattaacga gtggtaaatt ttaacttcaa tagaaaaaat attctgttat ttttaaacaa
     1141 ttaattagtc ataaagtata aaatgtgtat tatataagtt aatcagtact ttatgaagga
     1201 agaggagaga aagatacaat gaataattta gatgatatac cagttatgcc aactttgaca
     1261 cttgacccat ttggagaaga atcaaacact aatgatatag ataattctga cttattaatg
     1321 aaaaaggatg aaaaagaccc agaagaagaa aaactgtctg aatctgaacg caagatggta
     1381 aaggaatttg cggaaaaaat agatattaca aatacaaata tgattttgca atatggagct
     1441 ggtgctcaaa aaaaggtagc aagtttttct gaaacagctt taaagagtgt aaagacaaga
     1501 gatttaggag aagttggaga tatgcttaca aatctagtta ctgatttaaa agcattttca
     1561 gcagatgaaa aagaacaaag tggtttttta ggaattttta aaaaggcaaa taataaaatt
     1621 tcaaatttaa aaactaagta tgatagtgca gaggtaaatg tggataaagt aagcaaagag
     1681 cttcaaaagc atcaggttaa aatattgaaa gatatagcaa tgcttgataa gatgtatgaa
     1741 ttgaacttag cttactcaaa agagcttact atgtatatta tagcaggaaa acaaaaatta
     1801 aaggatatga aagaatatga gatgccaaag ttgagagaaa aagctcgttt atctggttca
     1861 acagaagatg ctcaatctgt aaatgatatg gtttcacttt gtgacagatt tgaaaagaag
     1921 attcatgact tagaattaac tagaatggta tcgcttcaaa tggctcctca gataagattg
     1981 gttcaaaata gtaataatct gatggcagaa aagattcaat caactatagt aaataccatt
     2041 ccattatgga aaaatcaaat agtgcttgct cttggaattt ctcacttaaa tcaagctatg
     2101 aaagcacagc gtgaagttag taatatgaca aatgagcttt tattaaagaa tgcagaaact
     2161 ttgaagatgg gaacaataga aacagcaaaa gaatcagaaa gaggaattgt agatatagac
     2221 acaattaaga aaactaatca atctctaata tctactattg atgaggtagt taaaatacaa
     2281 catgatggta gaataaagag acaagaagca gaagttgaat tatcaaaaat agaaaatgaa
     2341 cttaaaagta agcttcttga atttacagtt aaataaaaat tatactaata taaaaagaga
     2401 ctgtctcaaa atagagataa gtctcttttt cattgtttaa atgattaaag tatatttata
     2461 ttataaattt ctattatctt ttaacataac atcatgagct ccaccttcaa ctatactagt
     2521 agaagataca agtgtaagtt tagcattttt ttgaagttca ggaatgctaa gtgctccaca
     2581 gttacacata gtagaacgaa ctttacttaa agaaagagtt acattgtctt tcaaagaacc
     2641 agcataaggc acataagagt caacaccttc ttcaaaagaa agtttcttat ctccaccaag
     2701 gtcatatctt tgccagtttc tagcacgagc agaaccttct ccccaatact ctttcatata
     2761 actaccattt atattaactt tgttagttgg actctcatca aaacgagaga agtatctacc
     2821 aagcattata aaatctgcac ccatagctaa agctaaagtt atatgatggt cataaacgat
     2881 accaccatca gagcaaattg gaatataaat acctgttttt tcaaagtatt cgtctctagc
     2941 ttgagcaact tcaattatag aagtagcttg tccacgtcct ataccttttt gttcacgagt
     3001 tatacatata gaaccaccac caataccaac tttaacgaaa tcagcaccag cttcagctag
     3061 gtaaaggaat ccttcgcggt caactacatt tccagctcct accttaactg tatcaccata
     3121 tttttcacga atgaaatcaa gtgttatttt ttgccactca gaaaaacctt ctgaagaatc
     3181 tatacataaa acatcagcac cagcttcaac taaagcagga acacgttcag caaagtctct
     3241 agtattaata ccagctccaa ctacatatct cttagaagaa tctaaaagtt caagtggatt
     3301 ttctttatga gaagagtaat ctttacggaa taccatatac ataagattac cattatcatc
     3361 aagcacagga agagaattaa gtttgtggtc ccatataatg ttgtttgctt cctttaaagt
     3421 aacatctttg ttagcataaa ctatagatga cataggagtc ataaattcac taactttagt
     3481 atctaaatcc atacgactta tacgataatc acgacttgca acaataccca ataatttacc
     3541 agttgaagtt ccatcttcag taacagctac tgtagaatgt ccagtttttt cttttaagtc
     3601 aagtatgtct tttaatgtgt tatcaggttt aatatttgaa tcacttacaa caaaaccagc
     3661 tttgtgggat ttaacacgtg ctaccatagc agcttcattt tcaatagttt gagaaccgta
     3721 tataaatgaa ataccacctt ctttagcaag tgcaatagcc attttgtcat cagaaacaga
     3781 ttgcataata gcagaagtca aaggtatatt catataaata tctgcttcct cgcccttttt
     3841 aaatttagta acaggtgttt ttaaacttac attagcaggt atacaatcag tagaagagaa
     3901 acctggtaca agaaggtatt cactgaaagt atgtgatggt gtatcaaaat aaaacgccat
     3961 tttttaatca ctccttatat ttttatactt tttataattg atagtattaa ttataactta
     4021 ttctttaatt ttattaaagt attttgtcaa atattgagat ttttgaaaaa tttaaactat
     4081 tttaattaaa tttgtttaat atatattgcc ttatagggta aagttgtata ttaaataatg
     4141 tttagtgttt aatagtagct aatttaaaat taatctttag tgattcatat aattacattt
     4201 gtatagttta cattgataat attactagag agtgtataaa cctcaaaaat acagataaat
     4261 taaatctata ttagtatatc caaaagtaca tttaataaat ttatttaact gaattattat
     4321 tttcatttct attaaattta aatattttat aaaaatagta ttatttacaa aatcatatac
     4381 tccaaaatac tacataaata aaattaaaca aaaactaaaa tgacaaatgt cattatctaa
     4441 agtgacatag aaacggtttc ttaaagttgt tactataaaa tataaactct ataataaaag
     4501 tataacaaaa gggaatcata tcgatataga atataattca agaggaggta aaattatgag
     4561 tagttctaat caatcgacaa taaagacatc tatacagcga gtagggaaat ttttaagtgg
     4621 tatggtaatg ccaaatatag gagcttttat agcatggggt cttataacag cattatttat
     4681 accaacagga tggatgccta atgaaaaatt gagtactata ggtgacccaa tgataaaata
     4741 ccttttacca ctattgatag cttatactgg tggtaaagca atagcaggac aaagaggtgg
     4801 cgtaataggt gcagcagcag ctatgggagt aattgttggt gcagatatac caatgtttat
     4861 cggagcaatg ataatgggac catttgcagg atgggtaatt aagaagtttg ataaatttgt
     4921 agatggaaag ataccaacag ggtttgaaat gttagtaaat aacttttcaa taggtataat
     4981 aggtatgctt cttgcaatac taggatttta tgcaattgga ccagcaatag tagcagggac
     5041 agctcttata gaatctggag ttcaatttat agttagcaaa agtttattgc ctctagtttc
     5101 agtatttata gaacctggaa aagtattatt cttaaacaat gcaataaatc atggtatatt
     5161 agggcctata ggtatagctg aggcaaaaga agcaggtaaa tctataatgt tcttactaga
     5221 aagtaaccct ggaccaggtt taggagttct attagcatat tggatgttta gtagaggaag
     5281 tgtaaaacaa tcagcaccag gagctgtaat aatacatttc tttggtggta tccatgaaat
     5341 ttacttccca tatattttaa tgaatccagt tttaatattg gctacaatgg caggtggagc
     5401 agcaggaata ttgacttttt caatattagg agctgggctt gtagcagcac catcaccagg
     5461 aagtatattt gcacttatgg cactagctcc aaagggagga ttacttccag tattagcagg
     5521 agttgcagtg gcaacagttg tatcattctt agtggcagca ccatttgtta agagagcttc
     5581 agcaaatcaa agtgaagaag acagtacttc tttagaagaa gctaaagcaa aaatgagtga
     5641 tatgaaatct gcaagtaaga actctgagaa aaatattgag gaaaaacagc ttgaagtaaa
     5701 tgagattaag aagattgtat ttgcatgtga tgcaggaatg ggttcgagtg caatgggagc
     5761 ttccagattt aaaaatagaa taaaaaactt agacttaaat atagaaatta caaattcatc
     5821 tgttgataat cttccagatg atacacaaat agtagtaact cacaatacat tagttgaaag
     5881 agttgctaag aataattcga gtgttgagat agtttcaata aacaattttt taaatgaccc
     5941 aaatttagat acattattta aaagattaga atctaaataa aaataaaaag taccactaat
     6001 taggagtgga taatttgaaa aaaaagaaga ttacatctag gcaaaaaaag ataatactaa
     6061 tgatagtaga aaattcaaag aaaaacattc caataactat ttctgagatt gcagggactt
     6121 tagagttaag ttcaagaact gtacttagag atatgtctgg aattgaaaaa tggtttgatg
     6181 aaaatgattt taactttgta aaaaaaccag gagtgggatt gattttagag gaaaatatag
     6241 aaaatcaaaa ctttataatt gagttgttag aagaagaaaa aatagaaaaa gaatacagca
     6301 aggaagaaag aaatttaatt atattatcaa agctactagt ttctaacgag ccagtaaaat
     6361 cctattattt tgcaaaaata ttaaaggtat ctgaaggggt actgaataat gattttgcct
     6421 tagcaagtaa atggttggaa agatttgata ttgagcttgt tagaaaacca gggcttggtg
     6481 tctatttaaa aggtcaagaa aaaagcttta gagaggcata tgtaaatctt atatacgatt
     6541 cattcaatga aaaagaaata ctagatatgg tcagaaatat aagtgaaaat atacaaacag
     6601 ataaagcaat agaaatttta agtgaaaata gacttttaaa tttaatggac aggtgcataa
     6661 taagaaaagt tgaagctaca ttaacaaaga agctttcaga tttagatgta aatttagctg
     6721 atagtgcata tatagggctt gtagttcata tttcattagc cttacagagg ataaaaaatg
     6781 gtgaaaatat aactatggat aaagagtttc taaaagaatt atctataaca gaagagttta
     6841 aattagctaa agaaatcgta aaaggtatgg aaacagattt taacatggat ataccagtag
     6901 atgaggttgg atatataact atgcatataa gaggtgcaaa gcaaaggtca tcttcaaacc
     6961 ataaggcttt aaacttagat gatatagaaa taatggaaat tacaaataag atgatagatt
     7021 tggcggaaga tgaatttaaa ataagtttaa aaaatgacga gaggttattt aaagacctag
     7081 caaatcactt agggccttct ataaataggt tgaatatggg attagaaata agaaatcctc
     7141 ttttagatga aataaaaagc aagtattcat atgcctatga tggagttgaa aagatttcta
     7201 gaattataaa agataaacta aatataaatt caatacctga atcagaaata ggatatattg
     7261 caatgcactt tgcatcagcc atagaaaaaa atcttatgat gaatactaat ataaatattg
     7321 ttgtggcatg tcccactgga ataggtactt caagattctt atcaactaag attgaaaata
     7381 aatttcctaa tttaaatata cttgaaacca tatcagctat aaacatagat gaagagtatt
     7441 taaaggaaaa agatgttgat ttaatagtgt ctacagttga gttaaataca agtttaaatt
     7501 atatatgtgt aggtccattt atgagtttag atgatgaaca gataatcaag gaaaaaataa
     7561 aatctatagc tcaaaataaa ttaatcaatt taaatgcgaa aaatgatact aagagtaaga
     7621 ataaagtata tgaacagata acagaaagta tgaatatagg aaaagatatt cttcaatttt
     7681 tagaggaaat tagatttgaa aaatttgaaa gtaaagattt aagtgagctt attgaagact
     7741 catcaaaaat atttgcaaaa tctagtgaag atataatctc tataaaagaa tcgttaaaag
     7801 aaagattaaa aatttcaatt ccatatatag aagaatcaaa aattctattg ttacattgta
     7861 tgagtgaaag aatagatatt atgaagttat caataataaa actagagaat agaatcgtgc
     7921 tagattcaaa tgaagaaatc gacaatgtag tttttatgtt acttcctaaa aattcaccaa
     7981 gttatcaaag acaaataatg agtgaaataa gtggttcatt aatagacaat ataattttta
     8041 ctaataagat aaataaattc tctatagaag agatggcatt agaggtaaaa gatatagttt
     8101 ttaattttta tacaaacaga ttaaaagcat ttatagataa ataaaattaa tattaaaaag
     8161 gaaggttgat actatgagta aagtattgaa tgagaataat atatttttag ggttagatag
     8221 tgtttcaaag gaagaagcta taactttagc aggaagaaaa ttagttgaaa atggatatgt
     8281 aaaagaagaa tatataccag caatgttaga aagagaaaaa gtaatgacta catatatggg
     8341 aatgggagtt gctattccac atggtgtaaa tgaagcaaaa aaagaaatat tatcttctgg
     8401 aatagttata ttacaattcc ctaatggtat agattttgat ggagaaaaag catatttatt
     8461 aataggaata gctggtgttg gagatgaaca tttagaaata ctatctaata tagcagtagt
     8521 tttagatgat gacttaacag aaagactaaa aaattcaaat gacaaacaag cttttatgga
     8581 agcttttgcc aactaatctt aggagggatt taaaatgaaa aaggcaattc agtttggagc
     8641 aggaaatatt ggaaggggtt ttattggagg cttgttagtt aagtcgggtt atcatgtagt
     8701 atttgctgat gtaaatgagg atatattaaa ctcaataaac aaagataaaa agtatactat
     8761 acacataaga gatgtagaat gtatagatga agtaattgat aatataagtg cggtttcatc
     8821 tataaaagaa gaaataatag atgagattgt acaggctgaa ataataacta cagcagttgg
     8881 acctttagtt ttgactaaaa tagcctctac aattgcaaaa ggtataaagg ctagaaaaga
     8941 aaagggatta acaagtaatc taaatataat agcatgtgaa aatgcaatat acgcaagttc
     9001 ttctttaaaa gaagaggtac ttaagtattt aaataaggaa gaagtagaat atttagagat
     9061 gtatgttggt tttccaaact gttcagttga tagaatagtt cctcctggta aaaacgaaaa
     9121 tcctcttgat gtaacagttg aaaattttta tgagtggaat gttgaaaaac aaggatttaa
     9181 aggggaaata ccaactatag ttggaatgaa tttagctgat aatcttatgg cttatataga
     9241 acgtaaattg tttacactga atacaggtca tgctataaca gcttatatag gatatttaaa
     9301 aggatataag actatagaag agagtataaa agataaattt atctgtgata tagtcaaaag
     9361 tgcaatggta gaaagtggag aaggattgat taaaaaatac aattttgact cagaagtgca
     9421 ctataaatat attgataaaa ttttaaatag atttaaaaat ccatatctta atgatgatgt
     9481 attaagagtc ggaagggaac cattaagaaa actaagtgat aaagatagat tgattaaacc
     9541 tttaatgaca gcaaaatcat atggattatc agttgataat ttgattcttg gaataggtgc
     9601 agcgcttcat tataataata gtgaagatac tcaaagtgta gaattacaag agttgataaa
     9661 gtctattggg gtaaaaaaag ccgttgctaa aatagctaat ataagcaatg acgaagaact
     9721 tttaaataat atcgaaaaaa gttatatatt tatgaaaaat ttataaattt acttgaaaaa
     9781 aagtagttct gataaagtaa ctataatata ttattctaca cagtttgaat tatttgttga
     9841 ataatagaga attataatat ataattaaaa ggaataaatt aatatattta cctgtatatg
     9901 tttgataata tggttcaaat gtttctacta agctaccatt aatagcttga ctacaggagt
     9961 gaagaagata tagtttatac ttattttaaa ttatatatta agttaattac tttgaatttt
    10021 aagctcctgt ctatgatata gacaggagct tttttagtat tttaatataa aatacttaca
    10081 agaatagata gcaaaattag aacaaatata atttttttat taatataatt ttagaaaggg
    10141 ctaggtggaa gttcaatgaa ggctttacaa gaaaagatat tgagagaagg cagtgtttca
    10201 ggaaatgata tactaaaagt tgatagtttt ttaaaccatc aaattgatgt agctttttta
    10261 aatgaaatag gaagagaatt taaagagaga tttaaaggag aaaaggtgga taagatattt
    10321 acaatagagg cttcaggaat agctatagca tcaattgttt cacagtattt tgataatgca
    10381 cctgtagtat ttgcaaaaaa atcagaatct aaaaatttgg ataaagatgt atatgaaact
    10441 aatgtatatt catttactaa ggcaagagag tattcagtta aagtttctaa aaagtacata
    10501 aacaaaggcg aaaatatatt gatagtggat gattttttgg caaatggaag agctgctctt
    10561 ggacttaaag atttaattga gcaagcagaa gctaacttag ttggagtagg aattgtcatt
    10621 gaaaaaggat ttcaagctgg aggagcactt ttaaaggcta atgatgtaag attagagtct
    10681 ttggcagtag ttgaatctat agataatgga actgtaaaat ttaggtaatt tatgatatta
    10741 tatacttgtg tttagcacat attttaacta tgtaattatg gaggattttt tatgagattt
    10801 tttatagaca cagccaatat tgaagaaata aaagaagcaa atgatttagg tgtaatctgt
    10861 ggtgtaacta ctaatccgtc tctaatagct aaagaaggta gagattttat agaggtagtt
    10921 aaagaaataa gtgaaatagt agatggacca ataagtgcag aagtaataag tttagaacat
    10981 aaaggtatga tagaggaagc agaaaaatta tctaagatac ataaaaatat agttataaag
    11041 atacctatga cagcagaagg tctaaaagcg gtgaaagtac tatcaagtaa aggtataaag
    11101 actaatgtaa cgcttatatt ctctgctgga caagctcttt tagctgctag agctggagct
    11161 acatatgtaa gtccttttgt tggtagattg gatgatatat cacaaaatgg tcttgattta
    11221 attgaagaaa tcgttgatat atttagtgta aatagtattg aggctcaaat aatagttgct
    11281 agtgttagaa atccaataca tgttttacaa gctgctagaa tgggagcaga tatagcaaca
    11341 gttcctctta aagttataaa ccaaatgatt aagcatcctt tgacagacaa aggaatagat
    11401 agctttatga aggattggga aggtgcttct ttaaaattat aattataaac tacgctttat
    11461 aagtatatta aagaggttat tatggaaatt ctaagtaata aggctaggct attttgccta
    11521 gtcttatttt ggtgtataat atattgaaac atattattga aatagattaa ttttttaagt
    11581 gaggaggcga aaaatggata aaatattaag agagatacaa agagaagata aaaaaaatcc
    11641 atatactgac caagaacttg ctgaaatgtt aaatgtagct aggtctgaag tgatatcagt
    11701 taggaaaaaa aataatattt tagattcgag agaaagaaga aaaaaaatat taataaaaga
    11761 tatatcaaaa attgtaagtg aaaatcctca aatgtctgaa agaaaaataa ctgaaatatt
    11821 aatagaaaat ggatataata tatcgagaag tgcagtttca aaattattaa aagaggaaaa
    11881 tttaaatcag tcaaagattg ggattaagga taaagaccca gcatcatcag gaaagtatgt
    11941 gaaggtgagt aaagttgtag aagaagatgg atttgaagaa ttgataggta taaagggaag
    12001 tttgaaagaa aaggtaaatt tagctaagtc agcaataatg tatcctccta atggccttca
    12061 tactataata tatggagaaa caggagtagg taaaagtgaa ttagctacat gtatgtataa
    12121 atatgctata aaaaataaca ttaaagaaga gaacagccca tttatagtgt ttaactgtgc
    12181 agactatgca gaaaatccta atctattgat agctcaactc tttggggtag taaaaggtgc
    12241 atatacagga gcagatgcaa atagagaagg tcttgtagaa caagctaata atggaatatt
    12301 gtttttagat gaaattcata gattgcctgc tgctgggcaa gaaatcttat tttcattaat
    12361 agatagagga gaatttagaa ggttaggaga aagtagttct aatagaaagg caaatgttct
    12421 cataataagt gctacaacag aaaatccaga ttctaattta cttcaaacat ttagaagacg
    12481 tataccaatg gtaataaatt tacctaatat gagtgaaaga ccaaaatctg aaagatatga
    12541 aataataata aagttttttg aaagagaagc aaagcgagta aacaagaatt ttataataac
    12601 taaagaagtt atgtgtgcac ttatgaatta taagtgtact ggaaatatag gtcagttaaa
    12661 gagtgatata caagtaactt gtgctagagc ttttagtaaa tcgatatttt ctagcgataa
    12721 ggtaattata gatttagata gtttaaggga ttatataaaa agtggatatt atgattgtaa
    12781 ttgtaaaaat gaaaagtatt ctgagttttc agttgaggat atatatatag atattagtga
    12841 attggtgagt ggaaaaaata gaattttatc tgataatgag ataagtgaga tatataatta
    12901 tgcagaaaaa gagcttaagg ttttggaaag taagtgcttt tcagaagaag agttaaaaaa
    12961 tgcatttata gaaaagttag ataagaagtt taatgctatt aaaaacaata aaaatttatc
    13021 tgatagaaga agtcgaatag actggggagg acaattaaaa gagtctacac tggagataat
    13081 ggataaggta attgtattta ttaaggagca gtttaagcat gtaaatcaag gtctgtactt
    13141 ggctctagca attcatattg agcatgctat aggtaggata aaagatggaa aaactataat
    13201 aaatccgagt ttagataaaa taaaagcatc aatgccaatg gaatatgagt tgtcaagata
    13261 tataatgggt atagtagaag atttgacaca agtaacattt ccagaagatg agcttggata
    13321 tttggcttat tatataaata aattttgtta taatgaggaa agtataaaag ataaagttaa
    13381 agtagtaata gttacgcatg gtaaagttgg aattgaaatg tctaaagttg taaatcatat
    13441 tttaggtata gaatgtacac taggtataga aatagcactt acagattcac catcagaggg
    13501 gatagagcat gtattagaag agcttcaaaa gatagaagct agaaaaggta ttctagttct
    13561 aattgatatg ggttctttgg taattcttgg tgatgaggtt gaaaaaaggc ttggaattag
    13621 atgtaaaact gtaaatagag tagatacatt attggcaatg gaagcaggaa aacttgcaac
    13681 tattgaagga aaaagtttag atggtataat agctgattta aagaaaaata agaattatgc
    13741 tatgataaat acaaataaat ttagttacag aaaaaacgag tatggaaaga aaaatgtaat
    13801 cattacttta tgtttgagtg gtttaggtac agctttaaat ttaaaagagc atatagaaaa
    13861 acaaattaag gaatatgaca ctttaattga agtaaagcca gttgcttttt taaataataa
    13921 tttagaagaa gagttaaatg atattgaagc tgaatataat attattgcta tctgtggaac
    13981 tattgatata gattataaaa acgtgccttt tatttcttat aatgaggtac ttggtaaaaa
    14041 tggtattaat aaagtattag agcatttaaa tagtaaacaa ccagctttaa atattgaaaa
    14101 ccagttgaat aatttaatac atgaagattt gattttatac aattttgaag gaatttcaaa
    14161 ggagtatata attgatactc ttgtttcaaa acttgaagaa ggtggatatg ttgactcaaa
    14221 gtatatatta agtgtatata aaagagaagc aatgggaagt gttgtaatgg cctctaaggt
    14281 agccgtgcca catggacttc cagaaaacgt tataaagcct gcaatagcaa tcgcgaggtt
    14341 gaataaacct atagtatggg acaataaatt tatggtggat ttggtggtac tactggcact
    14401 taaagaaaat aataagaaag aaatacgaag cttattttca aagattaatg accagcatac
    14461 tttagaagtt ttattaaata ctgaagataa aaatgaaata aaagaacttt taagctaatt
    14521 attagtaccg agaagaaaga caaaaaaatt tgctttagta taaaatttaa aactactcaa
    14581 ttgctaagta gttaattact ctatataagg ctag
//
LOCUS       DAGSWI010000100        14611 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.100, whole genome shotgun sequence.
ACCESSION   DAGSWI010000100 DAGSWI010000000
VERSION     DAGSWI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.100"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            134..289
                     /locus_tag="MJF80_002762"
     CDS             134..289
                     /locus_tag="MJF80_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281412.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            419..1432
                     /gene="aroF"
                     /locus_tag="MJF80_002763"
     CDS             419..1432
                     /gene="aroF"
                     /locus_tag="MJF80_002763"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBZ0281413.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGVFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1454..2518
                     /locus_tag="MJF80_002764"
     CDS             1454..2518
                     /locus_tag="MJF80_002764"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBZ0281414.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2518..3831
                     /gene="aroA"
                     /locus_tag="MJF80_002765"
     CDS             2518..3831
                     /gene="aroA"
                     /locus_tag="MJF80_002765"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBZ0281415.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3809..4888
                     /gene="aroC"
                     /locus_tag="MJF80_002766"
     CDS             3809..4888
                     /gene="aroC"
                     /locus_tag="MJF80_002766"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBZ0281416.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGMELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4891..6087
                     /gene="pheA"
                     /locus_tag="MJF80_002767"
     CDS             4891..6087
                     /gene="pheA"
                     /locus_tag="MJF80_002767"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBZ0281417.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRALDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFPGKSGSFTKEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6134..6943
                     /gene="aroE"
                     /locus_tag="MJF80_002768"
     CDS             6134..6943
                     /gene="aroE"
                     /locus_tag="MJF80_002768"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBZ0281418.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6955..7488
                     /locus_tag="MJF80_002769"
     CDS             6955..7488
                     /locus_tag="MJF80_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBZ0281419.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7509..8348
                     /locus_tag="MJF80_002770"
     CDS             7509..8348
                     /locus_tag="MJF80_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBZ0281420.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            8616..10097
                     /locus_tag="MJF80_002771"
     CDS             8616..10097
                     /locus_tag="MJF80_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBZ0281421.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTNINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLLEDKIEKEMEYALESGQFIMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            10130..13567
                     /locus_tag="MJF80_002772"
     CDS             10130..13567
                     /locus_tag="MJF80_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBZ0281422.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            13585..14331
                     /locus_tag="MJF80_002773"
     CDS             13585..14331
                     /locus_tag="MJF80_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBZ0281423.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(14280..14588)
                     /locus_tag="MJF80_002774"
     CDS             complement(14280..14588)
                     /locus_tag="MJF80_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBZ0281424.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         6037 a         1442 c         2458 g         4674 t
ORIGIN      
        1 gagaagctga tttaagtaaa taaaaaaata ttttaaattg aaaatattta tagaaaatat
       61 tgacaattat attttctttg ttataattat taaacaagaa gaataataaa ttataaaaat
      121 ggaggataat actatgaaag gaatattaaa agaactaaca gcaaaacttt atagcttttt
      181 ttactttatg ttctggggct tttattttag cgctggctat ttcttttaca aaaatatatg
      241 tacttccttt ctggtgaggc cttattttag tgattttgag cataagtaaa ttttataaaa
      301 atgattattt tatatattta tataagattt agtgcttgat agattatttt agagaactca
      361 ttagtgagtt ctctttttta ttttaaatat aaaattataa tattgggagg tagtaaaaat
      421 gatagtagta ttaaaaatgg gagcagataa aaatgaagtt aaaaaattaa ttgaggctat
      481 tgggagagaa ggagtagaag ttaatcccat agatggaaca gaattaactg ttttaggttt
      541 agttggagat actagtaaaa tagatgccaa aagaatagaa gcaaataagg tagttgaaaa
      601 agttatgcat gtggtagagc cttttaaaaa ggccaataga aaatttcatc cagaaccttc
      661 aatcataaat gttaatggaa tggaaattgg ttcaaagaaa attgctatga tagctgggcc
      721 ttgttctgtt gaaacagaag accaaatagt atctatagct aaagatgtga aaaaatcagg
      781 tgcaggattt ttgagaggtg gagtattcaa accaagaaca tcaccatacg ctttccaagg
      841 attgaaatat gatggattgg atttattaaa aaaagcaaaa gaaaaaacag ggcttcctat
      901 agttacagaa ataatgtcaa cacaagatat agatatattt gaagaaaatg tagatgtaat
      961 tcaagttgga gctagaaaca tgcaaaactt tgatttatta aaagaattag gtaaaacaaa
     1021 taaaacaata cttttaaaaa gaggattatc tgctacaatt gaagagtggc taatgtcagc
     1081 agagtatata atggctggag gaaatgaaaa tgtagttctt tgtgaaagag gtataagaac
     1141 ttttgaaaca tatactagaa atactttgga tttaagtgca attttagctg tgaagaaact
     1201 tagtcattta ccagttatag ttgacccaag tcatgcagca ggtaaatcat ggatggttga
     1261 ctctttatct aaggcagcaa ttgcagttgg agcagatggt ctaataatag aagttcataa
     1321 tgacccagca catgcacttt gtgatggaaa acaatcgatt aagccaaatg aatatgatga
     1381 attgataagt gaattaaaga ctatcgcatc agcagttggt agagaaatat aatctataaa
     1441 aggatggtta aacgtggaag aaaaactatt agtaaattta aaagaaaata gctacaatat
     1501 atttattaaa aaaaatatac taaagaatat aggagaagaa ataaaaaaaa tatattttgg
     1561 aaataagatt tttattgtaa ctgatgaaaa tatagcaaaa ttatacttgg ataaagtaaa
     1621 aaataattta agtgaatctg gatttgaaat taaatcttta atattaaaat ctggtgaaga
     1681 aacaaaatca tttaatactc ttcctaaaat atacgatagt ttacttgact ttaagttaac
     1741 tagaaaagat ttgattatta ctttaggagg aggggtaata ggtgatttag gtggatttgc
     1801 agcagcaact tttttaagag gtataagttt tattcaaatt ccaacatctt tattagcaca
     1861 agttgatagt tctgtaggtg gaaaagttgg agttgacctt gagagaggca aaaacttagt
     1921 gggaagcttt taccaaccaa aggctgtgtt tatcgaccca aatgtattat tatcacttcc
     1981 agaaaaattc tatagagatg gaatggcaga agttataaaa tatggatgta taaaagacaa
     2041 aacttttttc aataaacttt taagtttaaa aaatagagaa gaagtaatgc aacatataga
     2101 atacattata cataaatgtt gctttattaa aaaaactgta gttgaagctg atgaaaaaga
     2161 ctttggagat agaatgcttc taaactttgg ccatacatta ggtcatgcta ttgagaaata
     2221 ctacaatttt acaggttata cccatggaga agctgttgcc ataggaatgt ataagataac
     2281 tttagaaagt gaaaagatag gaattacaaa aaaaggtaca tcagaactta taaaatcaat
     2341 tttaatgaat tataatttgc cttatgagat tgaattaaga gataataaat ctatagtaga
     2401 tgctatatct cttgataaaa aaaatctagg tgatacatta aaaataatac ttttaaaaga
     2461 tattggagag agtattatat ataatactac ttctaaattt tttcaggagg taagataatg
     2521 ggaagtctaa aaatatatcc aagtaaatta tctggagatg taaaaatacc accatcaaaa
     2581 agtatggcac atagggcagt tatttgttca tctctaagca atggaaaaag tagaatttct
     2641 aatatagatt tttctgatga tataatagct actataagag caatgacatc attaggagct
     2701 attatagaaa agaaagaaga tattttagaa atttcgggaa ttttttctaa agaaggtatt
     2761 ttaaatagag agaatcagtt gaatcaacct aaattgacaa tagattgtaa tgaatcaggc
     2821 tctacattga gatttttagt acctatatct ttaacatttg atggtgtaaa aaggtttata
     2881 ggtagaggta atcttggaaa aagaccactt gatacctatt atgagatatt tgacagacag
     2941 aatataaaat atagttacaa agaaaatcag ttagatttga ttatatcagg taagcttaaa
     3001 ccagatgaat ttagagtaaa aggaaatata agttctcagt ttatcacagg tttactgttt
     3061 atattgccta cgctagaaag tgattcaaag ataataatta ctactgagct agaatctaaa
     3121 ggatatttag atttaactct ttcaacgata aaagattttg gggtagaaat tataaacaat
     3181 aactataaag aatttattat aaaaggaaat caaacgtata aagcgagaga ttataaagta
     3241 gaaggagatt attctcaagg agcattttat ttatcagcag atgctatagg agaagatatt
     3301 agcatattag accttaaaga agattctctt caaggggata gtgaagtagt tgaaatttta
     3361 tctaggatgg gaatggaaat attaagagaa ggcaacaaaa taaaaggaat aacaaatggt
     3421 ttaaatggta ctttgataga tgcatctcaa tgcccagata taatacctgt attatctgta
     3481 gtagcatcct taagtatagg gaggactaca ataattaatg ctggtaggct tagaattaaa
     3541 gaatgtgata gacttcatgc aataaatgtt gagttaagta aacttggagc aaatatagag
     3601 gagaaagagg atagtttgat aatcgaagga gtaagcaagt taaatggtgg agtagaggtg
     3661 tggagtcaca aagaccatag aattgctatg acacttgcaa ttgcttcttg tagatgtgat
     3721 aaaccaataa tattaaaaga ttttgagtgt gtatcaaaat cttatccaca tttttttaaa
     3781 gattttaaga tgttaggagg tcgaattgat gagtggaata tggggaaata atttaaaagt
     3841 atcaattttt ggagaatctc atggaaatgc tataggtata aatatagatg gtcttccttc
     3901 ggggatggaa cttgaccttg ataatattga taaagaaatg aagagaagag cacctggaaa
     3961 aaattctata tctacttcaa gaaatgaaag tgatatacca gaaattctaa gtggatattt
     4021 taatggaaga actacaggaa cgccattatg tgcaatcata agaaatagtg atactcgttc
     4081 aaaagattat ggagagctta aaaatttaat gagaccagga catgcagatt tcacaggtaa
     4141 tgtgagatat tctggattta atgactatag agggggaggt catttttcag gtaggataac
     4201 agctccatta gtattttgtg gggctatatg taaacaaatt ttatcacaaa aaggaataga
     4261 gataggagca cacataaaaa aaattaaaaa tatagaagat atgagttttg attatgtaaa
     4321 tatttcaaaa caacagttat caaatttgca aactttggaa ctacctttac ttgatttatc
     4381 aaaggaagaa gccatgaaaa atacgattat agatgcaaaa aatcaaggag attcagttgg
     4441 tggtataatt gagtgtgcag tagtaggtat taatgtaggg cttggaaatc cattttttga
     4501 ctctgttgaa tctactttat cacatttatt gttttctgtt cctgctgtaa aaggtgtaga
     4561 gtttggttta ggttttgaac ttgcagatat gtatgggtct caaagtaatg atgaaatgta
     4621 ttatgaagga aatcaagtta agagcaaaac aaataacaat ggaggaatta taggagggat
     4681 tactactgga atgccaataa ttttcaaagt ggcaattaag ccaactccat caattagcag
     4741 acaacaaaac acagttaata taaaagataa aaaggacgat attttatata ttaaaggcag
     4801 acatgaccct tgtatagttc aaagggctat acctgtaata gaagcagtaa cagccattgg
     4861 aatttttgat ttgatgaaag gacgataata atggaggatt taacgagata tagaaatgag
     4921 attgacaata tagataaaga gcttattcaa ttatttgaaa agagaatgaa tacagttctt
     4981 gaaattgcca gacataaaat gaaaaataac actactatac ttcaaaaaga tagagaagaa
     5041 aaagtactaa gtagagcctt agataattta gataataaag catattcaca agaaacggta
     5101 cagtttttta attctataat ggaaattagt agaggattac aaaaaagatt gatagataat
     5161 gatggtgagc agaaaatcga gtttagttta gattcatcaa ataagaaatt tgatttgtca
     5221 aatgtaaata gatataagtc tttaaaagat gaattaaata aaaaaaatat cctagtaggg
     5281 tttccaggaa aatcaggttc atttacaaaa gaagcactaa ataaattctt caacaaaaaa
     5341 atatcaaaaa aacaatttaa agaatttgaa gatgttttca tagcacttaa aaataaagaa
     5401 ataaactatg gaataatccc catagaaaat tcatctacag gagctatctc tgaaacttat
     5461 gaccttttga gaaagtacgg tttttatata gttggagaag aatgtataaa aatagaccaa
     5521 aatttaatag ggataaaaga cacaaagctt gaagatataa aagaaatata ttctcatcca
     5581 caaggtatag gacaatgtag tgaattttta aaacaaaata gtacatggaa attgatacca
     5641 tttaataaca cagcaacaag tgcagaactt gtaagaaaac ttcaagataa aacaaaagca
     5701 gcaattgcaa gcaaaaaagc tgcaagtatt tatggattag aaataataag tccttgtata
     5761 aatgatattg caaacaacta tacaagattt gtagtaatca gtaatcaaat acacattgaa
     5821 gaagaatcaa acaaaatgag tgtagtattt tctgttgaac atgaagcagg aaaactttat
     5881 aaagtacttg gatactttgc taaaaataat ataaatatga caaaaataga atcaagacct
     5941 atgaaaaatg cctcatggag atatttcttt tatatagatt ttgagtgttc tatatacaat
     6001 gctaaagtgt acgatttatt agaattgata gaacataata cagcatattt taaatttatg
     6061 ggagtatata aaaataaaac tgaatagata agataaattt ttatttaatt tataattatt
     6121 aggaggtgta agtatgaatt ttttcggtct agtaggtgaa aaactctctc acagtgtttc
     6181 acctcaaatc cacaagagag tattcgagat attgaatatt gaaagtgctt ataaaaactt
     6241 tgaaatttca aaagaagata tatctaagct agacggtgct ataaaattat taggaataca
     6301 aggggtaaat gttactgtac catataagga gcgtattatg aaatatcttg attttatatc
     6361 accagaggca aaacgtatag gagcagttaa tacaatttta cttagagaaa atatgttata
     6421 tggttacaat acagattact ttggtcttga tagtatgttt aaaatggcta atattgatgt
     6481 tcaaggaaaa gtagcagtta tattaggaac tggaggtgct tctaaagctg ctcttacata
     6541 ttttattgat tctggtatag aaaagcttta tgtttcaact agaaaaaagg atgataaaaa
     6601 attattaaat agtaaagcta tattaataga ttatgaagaa cttaaacata taaaaggaga
     6661 tataatatta aatgctacac cagtaggtat gtaccctaat gtaggaattt caccagtgtc
     6721 aaaatctatt atacaaaatt ttgatatatt aatagattta atttacaatc ctggagaaac
     6781 agagttttta agaattggta atagtatggg taaaaaaact tgtgatggat tatatatgct
     6841 ggtggggcaa gcaataaaat ctcaggaaat ttggcaagat actaaaatag ataatagtat
     6901 tttagatgtt atatataatg aactaaaatt agaatttttg taaaggagat tgttatgata
     6961 aataaaacaa aagaaaaatt gatattaata ggtatgccag gtagtggaaa aactacaatt
     7021 ggtaaacttt tggccaaaga atataattgt agtttctgtg atatggatga ttatataatt
     7081 caaatatctc aaaaaagtat agcagagttg ttttcagaag gagaagatat ctttaggaac
     7141 tatgagactc aagcatgtag agaactttct ataagtgata aaacagtgat atctacaggt
     7201 ggaggtgtaa taaaaaagga tgttaatatg gaaatactaa aagaaacagg tataataatt
     7261 tttattgata gacctataca aaagattttg gaagatataa atattaattc aagaccatta
     7321 ttaaaaaacg gaaaagatag attgtataat ctttacaatg agagaataaa tctttataaa
     7381 aaattttcag atatagagat tttaaatgat aaatcattaa ataatgctgt atataatatt
     7441 acaaatgcag tcagtgaaaa ttttaaattt gactttaaag aaaaataatc tagggggaag
     7501 taaaaataat gaatattgtt attgtaggac taggagttat aggaggctct tttgctaaag
     7561 cattaaaaaa agctggatat gaaaatgtat ttggagtaga tgtagattta gaaactttaa
     7621 aaaaagcaga aaaagcaaaa atcataaaaa aaggatgtac tacaggtaaa gagttattta
     7681 aaaaagctga tttaattata ctatctatat atcctagatt agttgtagat tttctaaata
     7741 ataataaaaa cttttttaaa aaagggacta taattactga tactacagga ataaaagaaa
     7801 cacttatcaa tgatgtgttg caaattattc cagatgatat agattttata tttggacatc
     7861 caatggcagg tagggaaaaa aagggtattg attttgcaag tgaacaagtt tttaatggtg
     7921 caaattatat aattactcca actggcagaa acaacataaa aaatttagag ttagttgaaa
     7981 atttaatttt agagattgga tttaaaagag taaaaaagtt aacttcacaa aaacatgatg
     8041 agattatagc ttttacttct cagttacctc atgttatggc agttgctctt ataaatagtg
     8101 atgaagaagg tagagatacg ggtaaattta ttggagatag ttatagagat ttaactagaa
     8161 ttgcaaatat gaatgaagac ctatggagtg aacttttttt aggaaataga gataatttac
     8221 taaaagttat agaaaacttt gaatcagaag taaatttagt aaaagaagct atatttaaca
     8281 atgataaaaa taaattaata gagtatttta aaaaatcatc aataagaaga gaagctttag
     8341 aaaaataaat attaatattt aagctatata tagatgggta taaatataag cgatagtgta
     8401 tttaattgta ttaataaata tggattcaat ataattaaat atgctattga tatatttgta
     8461 cccttaacta ttgttatctt ttaaatgata atgatattta tttaaatact ctttattaac
     8521 aatttataaa acttaattgt aaaaaatact tattaatagt aaaataaagt gaatcaatta
     8581 tattataatt gctaaagaga gtgaggtgta gctatttgaa aaaaaataga ttaatagcaa
     8641 agaatatatc aatagcattt atagtactat tcttttttag tgtatttaca ttcttttatg
     8701 taggtaatat taatagagtg ttagaatatg aaacaaatga cataataaca gttacaattg
     8761 ctggatggat tatactttca tttttatttt taggtattat aatttacata ttatattcaa
     8821 aagcaaatag tcaaaaaact atagaaaaag tagcatatac ggattttgta acaggttatt
     8881 ccaactggag aaagtttgaa ttagatgtta caaatttatt aaagaagaca tcacaaaata
     8941 ataaatatgc aatggtcata ttcgatattg ataaatttaa agcaattaat gatatatatg
     9001 gccataaaaa gggaaattta atattaaaag atattgcaga tactttaaat gaattgacta
     9061 atatcaatga aacttttgct cgtgtaagtg ctgataattt caatatatta ttaacatata
     9121 acaaaaaaga ggatataata aatattatca aaaagattat ggcaaataat gaacttgtaa
     9181 acttatcttt tggaatttat gaaatcaaag ataaggattt atctgtaagt gtatatagtg
     9241 atagagcttc acttgcaaaa tcatcaataa aaaataatag tgatgtaaat tttgcatttt
     9301 ttaatgataa attaagagag aaattattac ttgaagataa gatagaaaaa gaaatggaat
     9361 atgctcttga aagtgggcaa ttcataatgt atttacaacc aaaatacaat attaaattag
     9421 ataaattttg tggaagtgaa gccctagtca gatggcaata tacagaaaaa gaggtcatat
     9481 atccaggtga ttttattcct atatttgaaa aaaatggatt tattagaaag attgatatgt
     9541 acatattaga acaagcttgt aaagaaatcc ggtctttatt tgataaagga atttcacctt
     9601 tacctatatc tgtaaacttc tcaagagttg atttttttaa aaaagatttt attgaaaaca
     9661 tagtaaatat atgtgataga tataaaatac catatagttt aatagaaatt gaaattactg
     9721 aatctagtat gtttggagat actgatactc tatttaatgt tagtagaaat ctacaggata
     9781 ttggctttat agttgcaatg gatgattttg gctcgggata ttcttctgta aatatgctta
     9841 aaaatatacc attaaatgta ataaaattag atagaggatt ttttgttgat gataaagatg
     9901 ttgataagag tcaaattgtt ataaaaagca tagtttctct cattaagcaa ttaggaataa
     9961 gggttgttgc tgaaggaatt gaaacaagaa gtcaaataga aatgttaaaa aaagcaaatt
    10021 gtgatattgt tcaaggatat tatttttcaa aaccattacc aattaaagaa tttgaaaaat
    10081 tagtctataa aatatagtca gcgagtctta aatgggaggt ggtgattaag tgataagagc
    10141 aaaatcgtta aaaaatgtac tgagtattgt tttaatattt ttatttacaa taatttcttt
    10201 ttctgatttt gcatttaaaa ctaatgtcca ggaagaagaa aatagtaaaa ttaatttaag
    10261 ggaaatatcc ctacaaagtt ccaatgctat aaaaataaaa ttagatagtt ctctatcctt
    10321 tattaaaagt gtggcaaata tggtttctta ttttaatact atagataact caaaaatagt
    10381 tccattatta gctaatatat caaaagaatc tgactttcaa agaatctcta ttgtatctcc
    10441 tgatggaata agtcatacca gcgatggcaa aattatcaat gtgtcaaagt atcagtattt
    10501 taagaaagct attaaaggag aaagtaatat atctgaagtt gtgaaatctc caattgatgg
    10561 aaaagatact tttgttatag ctactcctgt atttaataag aataataaaa taattggtgt
    10621 tttatacggt tcttactaca atgataaatt agctgaattt atagatataa caagttttaa
    10681 tagtgaaggg tacatagata tatttgaatc tagtggtcaa tttgttttaa aatcttcaca
    10741 tacaaacaaa ctgattaagt caagtagtaa catatatgat atatttaaaa aagcttcttt
    10801 tgatgatggt tatagctata aaaaaatata tgaagataca aaaaataaca agagtggttt
    10861 tacgtcatat gaatacaaag gagaaaatcg ttatgctact tatacaccca ttggtgtaaa
    10921 tgactggtat gtaatttctg tgataccaca atatactgta agaagaaaca ttgataaaat
    10981 aaatcaatta gcattttctc ttattttcaa ggtattctta ttgtttatta tgcttataat
    11041 atatattatt tataaaaaca ataaatctca gaaagatata ctaaaagcta acaaagaaat
    11101 ctcatctatt acaaatagca ttcctggagg agtacaaaag tgtaccctgg gtgaaacatg
    11161 caatattaat tttttaagtg atggatttat agagttgttt ggttatacaa gagaagaaat
    11221 aaaaacattg tttaataatg atttttatag tttaatacat aaagatgatg ttgaaagaat
    11281 aaaaaaagaa atttttaaac aactttacaa aaataaaact gttgaattag aatataggat
    11341 tataacaaaa aatggtaata cagtatgggt tttagataaa ggggagctta taacagaacc
    11401 tgatggaaaa gaatattttt gttgtgtaac tgtagatatt actagaacaa aacaattaga
    11461 acaagagatg gaacaaaaaa atctagaact tgaaatgata tcagaaaatg ttttaggtgg
    11521 tataattata acagaattta atgatgattt tactataaaa tacgctaact ctggttatct
    11581 ttcaatgatt ggatatacac gtgaacaatt agaaaatgaa cttaaataca aagcttgtaa
    11641 tcttgttttt tatgaagata gaaagaaggc tcttgaaagt atacaacatc aattaaacac
    11701 aaaaggaata gctgtatatc aacatagaat aagaagaaga gatggtaata ttatttgggt
    11761 ttctataaaa ggaaggctta ttgttgatga tgatggtatt gaaaaaggta tttggattct
    11821 aaatgatata acagatttaa aaaatgcaga acaaaaactt aaaataaatg aagaaagatt
    11881 tagaattgca ttaaagcaaa catcaaacac tatatttgag tatgatataa aaacaaagtc
    11941 acttttaaat actagtaaag agcttgataa atataatatt tataaaatta ctaaaaacgt
    12001 tccatattct ttagtagaat caggagttat tcataaagat tttagtgatg attttataaa
    12061 catgtatgaa aatgtaataa atggagcaaa aaaagcaagt tgtgtagtta agatgaaact
    12121 tccagatgaa aattatatat ggaataaagt taccttgact actatttttg atgaagatgg
    12181 taatcctgta agagcaattg gtatacagga agatataagc aaacaaaagg aagcagagat
    12241 aagatacaat caagaaggta agtatcattc agctatattg tcggaagcaa tgataaccta
    12301 tgaaataaat gtatcaaagg atacttttat caaaggaaat gaaagttggc aaaaaccatt
    12361 tggaattaag cctgacaata actattctca aatggtaaaa actatagcaa atagtattgt
    12421 acataaagac aataaagaaa catttttaaa tacattttta tgtgaaaact tattggatgc
    12481 atataaaaat aatgtgactg aaataaaatt agaatatcga agaatgaatc aagaaaataa
    12541 aatgatttgg gtcatttgta caatgcactt aataaaagat tctattacga acgatataaa
    12601 agcacttgct tatgtcaaag atattgacaa gcaaaaaaat gaagaaatta gattaaaatt
    12661 aaaagctgaa agagatactc ttacaggatt atataataaa ggtactacag aaaaattgat
    12721 taatgatttc ttgaactctg aggaagcaag cttaggaatg catgcatttt taattgttga
    12781 tatagataat tttaaatctg tgaatgataa tttaggacat gtatttggag ataaagtatt
    12841 atttgaagtt tctaaaaacc taaaacctat atttagaaaa gatgatatag taggtagaat
    12901 tggtggagat gaatttatag tattcttaaa aaatattcag tcttttgaga atatcaaaaa
    12961 gaaagcagaa aaaatatgcg atatatttag aaattcttat acagaaaata aaaagacata
    13021 taaaatatct ggaacagttg gaatttcatt tgtaccagaa catggaactg tatttgaaga
    13081 actttataaa aaagcagaca tagcacttta ttatgcaaag agcaaaggaa aagataagta
    13141 tgcactttat aatgattgta tctaccaaaa tgaacttgaa aataatataa gtgataataa
    13201 tataaaatta gcagataaat gtgagaaatc caaaccagta cttaaagaaa ttcttgattc
    13261 tattgataat tatatctata tagtgaatcc aaatacttat gaacttcttt ttgtcaataa
    13321 taaggtgtct cgtttggatt tagagataaa attaggagaa acctgttata aatcactatt
    13381 aaaatctgat agaccatgta ataattgccc tcttaacgga ttagaagaaa ataagcattc
    13441 aaaattcaaa atagagacaa atacatttgg taaaaatatc agtatagcaa gttggattaa
    13501 ttgcatagat ggagaataca actgtcttat agagtgtata gattcttctt tatacaaaaa
    13561 taaataaaaa aggactatga agatatgtta aatagtataa ctttgatact tggtataata
    13621 tttacaattt actattttta tctcaaaata gtttttggag gaatttcatt tagtgagatt
    13681 ttcttagttg taggaattgt actaattatc tatcaaatat ttaagaaaaa aattaaagaa
    13741 aagaaggttt tttatagggt gttaaaaata ataatcagca tttttttaat agtatttgta
    13801 gttactgaga gtctaattat tttttatccc aaaaatagtt tagaatcaaa aagtgattat
    13861 ctcttaattt taggagcttc tgtaaaaaag actacaccaa gtacaaccct gaagggtagg
    13921 ttagacactg cattgaaata cctaaaaatt aatgataatt gttatgtggt agtatctggt
    13981 ggtaaaggaa gtggagaaaa aataactgag gcaaaagcta tgaaagatta tcttataaaa
    14041 aatggtatag ataaaaatag gataatagaa gaggataaat ccacgaatac ttatgagaat
    14101 tttaaatatt caaaattgaa gatagaagaa catagtcaag gaaaactaag cgatttaaaa
    14161 gtaaaaatag taacaacaga ttttcatgtt ctcagaagta aaatattagc atatagaaat
    14221 ggatataaaa atacgtcttt ttatgctagt aaatcaaaac tttcatttgt accaacttac
    14281 tatacaagag aattttttgc attatggaaa acaatcattt ttgatagata ataaataaaa
    14341 agactagata taaactcata tctagtcttt tctattccct taattctaat ccattttcca
    14401 actttacttg ttgtagaaat ttcaacattg ggatttacaa gtaattgctt ataagaagtt
    14461 ttatatttta cgattccaaa atataatttt ccttcataat ccattataaa tccgaatggt
    14521 cttactcttg gtttattatc ttcaactgta gcaaaataga atgttgagtt ttctttaaaa
    14581 aaattcatta ttttttcatg attaaatcct c
//
LOCUS       DAGSWI010000101        14497 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.101, whole genome shotgun sequence.
ACCESSION   DAGSWI010000101 DAGSWI010000000
VERSION     DAGSWI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14497
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.101"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            953..2311
                     /locus_tag="MJF80_002775"
     CDS             953..2311
                     /locus_tag="MJF80_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBZ0281425.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2418..3212
                     /locus_tag="MJF80_002776"
     CDS             2418..3212
                     /locus_tag="MJF80_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBZ0281426.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3205..5511
                     /locus_tag="MJF80_002777"
     CDS             3205..5511
                     /locus_tag="MJF80_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBZ0281427.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5650..6927
                     /locus_tag="MJF80_002778"
     CDS             5650..6927
                     /locus_tag="MJF80_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBZ0281428.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7110..7832
                     /gene="yqeC"
                     /locus_tag="MJF80_002779"
     CDS             7110..7832
                     /gene="yqeC"
                     /locus_tag="MJF80_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBZ0281429.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            7939..8508
                     /gene="mocA"
                     /locus_tag="MJF80_002780"
     CDS             7939..8508
                     /gene="mocA"
                     /locus_tag="MJF80_002780"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBZ0281430.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8505..9524
                     /locus_tag="MJF80_002781"
     CDS             8505..9524
                     /locus_tag="MJF80_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBZ0281431.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            9862..11487
                     /locus_tag="MJF80_002782"
     CDS             9862..11487
                     /locus_tag="MJF80_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0281432.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            11686..12162
                     /locus_tag="MJF80_002783"
     CDS             11686..12162
                     /locus_tag="MJF80_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281433.1"
                     /translation="MVKKRLVAVVLSLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(12370..13482)
                     /locus_tag="MJF80_002784"
     CDS             complement(12370..13482)
                     /locus_tag="MJF80_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBZ0281434.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            13823..14200
                     /locus_tag="MJF80_002785"
     CDS             13823..14200
                     /locus_tag="MJF80_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0281435.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         5672 a         1492 c         2559 g         4774 t
ORIGIN      
        1 cttgtatatt ttatgttgca gatacaaata tagtaaagga gagtacattg gtacaactga
       61 gatacatagc ttattaagtt tttctgtctt atgagaatta tattataaaa tattcataaa
      121 tattttgagt ttaaattaaa taattatttt atgcttagat taattgatat ttaattaatt
      181 taataacaaa aacagtgaaa gagtaaataa tattatttac agggggtaaa gcaaagtatg
      241 ttaaataaat tgtacttttg gccccgtaca tagagaaatc tatgtaggac acaactttaa
      301 aaccagtaaa tcctaaagcc ttgcaactac aataaaagat agaaagaaac ttttatgaat
      361 gtggtgaaaa ccagaaacaa tagtgaggat ggtatatggt gagagcctaa gtacttaaaa
      421 atggacattt ggtcgccaag cctagaaata ggaaggtcaa acgactatct cgagagagag
      481 tacactataa gcgattgata gtggaaacgg gttgctctta gcaggtaatg ctgaagatga
      541 agaaatagtc tacgctcata tgaaagtatg aggtcactgg atttgaagtg acatctataa
      601 agtagcgttt ataggtaaat gtttaaaact tgaaacatgt aaatacttat gaaaacttat
      661 aacttataat gattaagatt gaaagattta aattgaatta aatttactat aaaaaattaa
      721 aaattcatag ctataatact cttatatttg taaagcatgg tatataatgt atacaaaatt
      781 aaagaatacg tttatttgtt aataattaaa tttattgcat ctgtttttta agactaaaaa
      841 cttaaatata tgttggagta tagttgatat ttatgaacat cattaattta atatattaga
      901 tttttaagta tttatataat tcaataagtt ttatgtaacg ggagttttta ttatgaaagc
      961 aaaaagtaca tcaaaatttg aattagacgg aataccacca ctaaaggaag caataccttt
     1021 agggttacaa cacttaatgg caatggtaat aggtagttgt ttaccagcga tattgattgc
     1081 aaatgcagct ggacttaatc ataagatgtc aacattattg attcaagccg cattagtttt
     1141 tgcaggatta tcaactttat tacaattata tccaatacct ttgtttaaag gttttaaatt
     1201 aggaggagga ataccagtta taatgggagc tacagctatg tttataggag ctggggtatc
     1261 tgttgcaagt aagtatggac taccagtatt atttggctct caaataatag ctggtatagt
     1321 aataattata attggttttg gtcttaaaaa aatacgattt atatttactc cagctgtatc
     1381 aggaacaata gttgcatgta tgggtcttgg actttttcca atagctataa aaaacttagc
     1441 tgggggtatg ggcaatgaga cttttgggaa tccaattaat tttatagttg gaatatctgt
     1501 atctcttatg atattaggat taaataaata tggaaaagga ttatttaaag atgcatcaat
     1561 attagttagc ataatttttg gatacatttt atcattaatt ttgggtatag taaatttttc
     1621 ttcaattcag gagtttactt tggttgcact tccaaaacca cttgcttttg ggctagatat
     1681 aagacttgaa gttgttgtaa tgttttcaat aatatattta gttgagatag ctgatataat
     1741 gggtgcttgt acactttctg cggtaggagg gcttaataga caagtaacag atgaagaact
     1801 ttcttctgct gttttaggaa atggagtact ctctacaata ggtgcattat tttctgcaac
     1861 tccaatgggg atgtttggac aaaattctgc aatcgtttcc aatacaaagg ttgtaagtaa
     1921 atttgtacta gctattggag gaattggatt atttttggca ggaatatctc ctttattagc
     1981 taatttaata agaacaattc ctccctgtgt ggttggagga gcaacactcg ttatattttc
     2041 aactcttaca acatctggtt taagacttgt tagtatggat ggatttaatc aagaaaatag
     2101 catgatatta ggtctttcta tggcttctgg tattggattc atggtagctc ctcaagttct
     2161 tgaaaaattt ccaaaattta tagagacatt acttgcagat tcaagtgtag tttcaggagc
     2221 aatggtagcc ataataattc aagctttata tatggtaaaa tttcctggaa ataaaagtga
     2281 taaggttgag gataaaaata aaagtttata atgtaaaggt ttaatatatg tgaattaaat
     2341 atactacaat tataaatata aataaaagat ttattaaaaa cttttgtgtt ttaataagtt
     2401 aatgatgggg ggataagatg gttactataa aggaatattc agttccgcag agtttagaag
     2461 aagcatataa aattttaatt tctaaaaaaa ataatgttat tttaggtgga tgtggattta
     2521 taaaattaag taataaaaat ataggaacag cgatagattt aaaagatata aatctaaatt
     2581 atataaaaga ggatgaaaaa aatattttga taggtgcaga tacttcactt agaagtttag
     2641 aaattgataa aactataaaa aattactgta gtggaatact ttcaagtgct gtttctaata
     2701 tagttggtgt tcaatttaga caaggggcta gaattggtgc aagtgtattt tcaaagtatg
     2761 gcttttctga cttaatacca gcacttttgg ttgttggagc gaaggtgaga ttatacaata
     2821 aaggaatact agagttaagt gagtttttgg atagtgaatt aagtagggat atattggtcg
     2881 aagtaatact accaaaaaaa gatgcaatag ctgtttttga ctcaataaga aaatgcacag
     2941 gagatttttc tgtattaaat tcagcaatgc taaaagaaaa tgatacttac aaaatagcaa
     3001 ttggggcaag acctcaaaaa gctagattag cacttgaagc gagtaatata ttaaataaag
     3061 aaaaagatat agataaggca tctattgttg ctagtaagga acttacttat ggaagtaata
     3121 tgagagctag taaagagtac agaaaggata tggcaagtgc attagttaaa aggatgtata
     3181 atgctattga ggagggaatg tataatgatt aataagggga taagaaagat agatagtaca
     3241 gcaattgtca caggaaaacc tctatatact gaagacctca ttatgcataa agatgttttg
     3301 acattaaaac tacttagaag tccacatgct catgctaaga taaagaatat agatacatca
     3361 aaagccgaaa aaattccagg ggttgtgggt atttatacgt ataaagatgt accacaggtc
     3421 agatattgtg ttccaggtca aacagaacca gaagcatcac cttatgatag agttttactt
     3481 gaagatacag ttagatttat tggagatgaa gtagctttag tagctgctga agatgagaaa
     3541 acagcactta aagccttaaa aatgataaag gtagattatg aggttttaaa gcctgtgttt
     3601 gatgcaagag aagcagaagg aaatgaagtt gttatacatc cagaaaaaga catagtagag
     3661 ccataccctt ttggattgga tgcaaaaaat aatatagtat caaaagaatt ttttgaattt
     3721 ggagatgttg aagctaagct taaagaaagt gattttgtgg ttgaatcttc atacaaaacc
     3781 caaagtcaat cgcattgcat gatggaaact cataggtcat attcatataa agatataaat
     3841 gatagaattg ttgtagtttc atcattacaa tcaccacaca atgtaaagag aatacttaga
     3901 aaagtattgg gtataccgtc ttcaaaaata agagtaatta aacctagaat aggtggtgga
     3961 tttggaggaa agaatatagc agcaacagat atatttactg catttgttac tatgaaaact
     4021 ggtagaccag caaaatttgt atttacaaga agtgaaactt ttgtaggttc taatagtaga
     4081 catgaaatgt tttttgatgt taaagttggt gcagataaag aaggaaattt aaaagcaata
     4141 gagctaaagt cattaaacaa tacaggtgct tatggtggga atggtgtctc tgtttcttcc
     4201 gaatctggac acaatacctt accaatatat aatgatattg atgctgttag atttaatgct
     4261 agaacagtat atactaatag gttaccagca ggagcactta gagggtatgg tgctacacag
     4321 ggaatatttg caatagatag tgctattgat gaattggcac ataagattgg aatggatcca
     4381 cttgaattta gaattaaaaa tataatagat aggtatacca atggaaaagc agctgaagac
     4441 gaaatattaa gttgtgagct gagacaatgt atagaaaagg gaaagaaatt aattaattgg
     4501 gatgataaat atccatgtaa agatatagga aacaacaaag taagagcagt aggtatggca
     4561 gtagcaaatc atggttctgg tataccgcgt gtagatatgg caacagtcac tataagaatg
     4621 gatgaagatg gcacatataa actattatct ggtcttgctg acttaggtca aggggctgat
     4681 acaattcaag ctcaaattgc tgcggaagct ttaaatacta ctgtagatag aatttctatt
     4741 tatacaggtg attcagattt atgtccctat gatacaggag catttgcttc ttgtacaaca
     4801 acggttacag gaaatgcaac aataaagaca gcaaataaat taaaagatat attactttca
     4861 gttgcagagt caaagctagg atttaaaaaa gaagatttag agttgggaaa agacagggtt
     4921 tttgttaaaa atgataatag caagtttgtt ctattagagg aattgggtag agaatctgtt
     4981 ggaggaatag gatttgctag tgatgatgta ccagtagttt tggaagcaac agaatctttt
     5041 ggcatgttga aaaaaataaa accatttata gcaggatttg cagagataga attagataaa
     5101 aatacaggcg aatataaagt cataaatttt gcatgtgtac ctgattgtgg aacagttata
     5161 aatccaactt tagctagaat acaagtagaa ggtggtgtga tgatgggtat agggtttgca
     5221 atgtatgaag accctatatt tgataaagat ggtagattat taacagatag tttcctacag
     5281 tataaagttc ctacaaaaaa agaagttgga aatatattgg tagattttgc tgagaatttc
     5341 gacccaggag gtccttatgg tgctaaatca ttaggagaaa tagttataca tacaccagca
     5401 ccagctatag caaatgcaat atttaatgct acaggagttc gtttaagaga gcttcctatg
     5461 acttttgaaa aagtttatat gggtatgaaa aataataata aacaattata ataaataata
     5521 aggtgtataa aagcaagtaa atttaaatta ttttgtaaaa taaagtaatt aaatggacat
     5581 tatatatggt gttttgtaaa aataccgtat ataatgtcta gtagagtata ttctaaagga
     5641 ggtgagttca tgtcaaacag aataaaaaat atacactatg gaactcagag acaacaatta
     5701 aaaaacttaa ctaaaaaaga atatcaagct ctacgagaga tgtgcttctt agctaagaac
     5761 ttatataatg ttggtacata taatgtaaga cagcattatt tcaatcaagg agaacatctt
     5821 aactatgaag ctaattatca tgtatgcaaa gaaaatgaga actataagct tctcaatagt
     5881 aatatagcgc aacaaatatt aaaagaagta gacagttcgt ttaggtcatt ttttggatta
     5941 attaaattag ccaaagaagg aaaatatgac tttagaggga taaaatcacc taaatattta
     6001 gataaagaag gattttttag cataataata ggtcaaataa gaatcaaaga agatgggatt
     6061 ttaaatgtac ctatgtctcc agcatttaaa aaactatatg ggaaagtaag tataaaagtt
     6121 ccacaaaata tactagagaa aaaaattaaa gaaataagaa taatacctaa acataaagct
     6181 aggttctttg aaattcagta ttgttatgaa ataccaaaat cagaggaagt ttcaaatcaa
     6241 aaaaatgcac tagctataga cttgggttta gaaaatttat gtacatgtgt taccaattta
     6301 ggtgatagtt ttatcataga tggaaaaagg ctaaaatctg tgaatcagtg ggcaaacaaa
     6361 cagaatgata aaatagaagc cttaaaatta aaaaagaata taactgcaat aacaagaaaa
     6421 caatttaaag tttggaataa gagaaataat caagttaaag actatgtaaa taaaacctgt
     6481 ttttatataa ttgattattg tgtaaaaaat aatattggaa atttaattgt aggatataat
     6541 gctaagttag aaaaaagtga taatatggag agaaaaatat atagaaattt tataagaatc
     6601 ccatttggag agataaaagg aaaattagaa tatctgtgta aaataaaaaa tatcaatttt
     6661 attaggcaag aagaaagcta tactagtaaa tcagattttt ttgctgatga ttttctacct
     6721 gatatcaatg tggacaatgt taaaaaatat gactttaaag gggaaagaat aacgcgtgga
     6781 cagtacaaat ccagtgttgg aatcattata aatgcagatg taaatgcgag tcttaatata
     6841 ctaaaaaaat cacaagtagt taatttatct aatttacaga ataatcctca aatattaaag
     6901 caaccacaga gagtaagaat atcttaatta tatttaaaag ataaactgat agcgtaagct
     6961 tacagcttag cggcgaattg gatccgcctt gttgtttaca acagaatcta tttactttcg
     7021 agtttatgga atttcaaaaa tcacttttac taaaaaggtc taactataat aattaagaga
     7081 ttagaaaact ggttataaga ggtgaaagtg tggaactatc aaatataata gaaaagaatg
     7141 aaataataac tgtagttgga gcaggaggaa aaacttcgtt tataaactat tttgcaaatt
     7201 tttatagaga taaattgaaa gttcttctta caacaacaac taaaatatat gtaccaaacg
     7261 attatgacaa tataattatt acaatagatg gaactgttat tccatctatt tgtcatggaa
     7321 taacagtatg tggtagttat ataaataatg aaaataaatt agtaagtatt gattcctcta
     7381 ttttggatga aattgtagac caatttgatt tagttttaat agagggagat ggctcaaaga
     7441 gaaaaaaact taaaggttgg aatgctaaag aaccagtagt atatcataaa actacaaaaa
     7501 ccatagggat attagatata acatcatttg gaatgaatat taatgaagag aatatacata
     7561 gagtagaaat atttaaaaaa atagcaaatt tagatacaag tagtattaat agtagttcta
     7621 cagtaagtat tgaaaactta aaaaatatag tattaaatcc aaatggatta tttaaaaatt
     7681 attctggaaa aagagtatta tttattaata aagttgaaaa tgaaaaatat aaaaatttag
     7741 ctataaaatt aatagaaaat attaaagaat atgaaagtga aatagagata ttttatggaa
     7801 gtgtaaaaca aaaattttgt gtaagatatt gaagtattaa aatagtttat atgatattat
     7861 atatatagat aatatcaata gagatattat aaaaatcata gaactatcaa tatattataa
     7921 ataccaagga tgatatatat gataaatgca gtgataatgg catcaggctt tgccagaaga
     7981 atgggagcaa ataaactttt attaaattat aaaggctgtt ctataataga acatgtattt
     8041 agagaagtta gcaagattga ttttcatcaa gttgtagtag taagtcaata tgaagaggtt
     8101 ttgttattgt gtaataaata tggttttgaa tatatagata ataaaaatgc aaatataggt
     8161 caaagtgaat ctataaaatt aggagtttta aatagctcaa aatgtgatgg ttatatgttc
     8221 tttgtaggag accagccatt tattgataac ttatatatag ataaaattat aaatactttt
     8281 aaattagata gagattttat agtaattcca agatacaaag atatgtgtgg aaatccagtc
     8341 atattcccat atagtaagaa agaagaattg ttggcactta aagaagatga aaaaggtaaa
     8401 accattttaa aaaattcatc aaaaataaag tatgtagaag ttcctaaaga aatgttattt
     8461 gatatcgata caaaaatgga ttatgagaga attgggggta aattatgaag ctaataaaaa
     8521 ctatagatgc tgttggacaa gttttatgtc acgatataac tcaaataata cctggagaat
     8581 ttaaaggaag aaaatttaaa aagggccaca tagtaaaaga agaagatatt ccagtgttat
     8641 tatctcttgg taaagataat ttgtatgtat gggaaaagtc agaaggaatg gttcatgaaa
     8701 atgaaggagc attgttttta aaagaactaa ctgctggaga aaatttagat tttagtgaaa
     8761 taaaagaagg gaaaatagat tttatagcag catgtgatgg tctattaaag atagatgttg
     8821 atgcactttt tgatttgaat tgtgttggag aaataatgat ggctactctt cataataatt
     8881 ttatagtaaa taaaggttta aaagtagctg gaacaagagt gataccttta gttatagatg
     8941 agaaaaaatt agaagatgca aaaaaagtag ttggtaatag aaaaatagta aatgtagtac
     9001 cttttaaacc taagaaagta ggaattgtta ctacaggaaa tgaagtgttt tatagcagaa
     9061 ttgttgataa atttggacca gtaatagaag aaaaagtaaa aggctttggt tgtgaagtta
     9121 taggacagac tatatgccca gatgataaag agattataaa gagtgcaata aaagaattta
     9181 taagtcaagg agcagaactt atctgttgta ctggtgggat gagtgtagac ccagatgatg
     9241 ttacgccaac agctataaaa gaaactggag ctgatttagt gacatatggt tcaccaatat
     9301 taccaggagc tatgttttta cttgcttatt atggagaagt tccaattatg ggtataccag
     9361 gatgcgcaat gtatcacaaa actacagtat ttgatatagt tttatctagg gttttaattg
     9421 atgaaaagct tgataagtat gatatagcta gatatggtca tggtggcctt tgtatgaact
     9481 gtgatgtatg tacttatcct gcatgtaatt ttgctaaaat ataaagttat taaaaaagag
     9541 tatttacaaa ttaagtactc tttttaaata tgaaacaaaa cagctagaat gatgttttaa
     9601 aaaaagcata tttatttaga aattataata acttattttc aatgtgtaaa agaatgaata
     9661 aagttgcaat ttgatttctt tataagtata atataagaga ttgttaattt ttataaattt
     9721 acaattttta gtattatttt aataaatatt tcacaaattt agtataaaaa atatactatt
     9781 tacgtgataa tatataataa aaatgttatc aaagttttat gataaaataa attatgagca
     9841 aaaagaaagg aaaatatatt aatgttacaa gttacaggtg ttggacttag atttggtgat
     9901 aaggaactat ttaaagatgt taacttaaaa ttcactaaag gtaattgcta tggaataata
     9961 ggagctaatg gtgcgggaaa atctactttt ttaaaaatat tatcaggaga aatagagcct
    10021 aatacaggta gtatatctat aactgataaa gaaagaatgt cagttttaaa acaggaccac
    10081 tttgaatatg aagaagaaac tgttttaaat gttgttataa gaggtcatga gagactttgg
    10141 aacataatga aagaaaaaga tgcattgtat atgaaggaag actttaatga agaagatggg
    10201 ataaaagctg ccgaacttga aggtgagttt gcagagcttg acggatggga tgcagaaact
    10261 aatgctgaga aaatacttat gggtcttggc ataacgaaag atatgcatta caaacaaatg
    10321 aaggaactag ttggtgggga aaaggttaag gtattgttag cacaatcact ttttggtaag
    10381 cctgaaatac tattaatgga cgaacctaca aaccacttag attttaaatc aataaactgg
    10441 ttaaataatt ttataatgga tttagaagaa tctatagtta taatagtatc acatgataga
    10501 catttcctaa atcaaatatg tactaatata gttgacgtag attttggtaa aatacaaatg
    10561 tacgttggaa actatgattt ctggtatgag tcaagtcaat tggctttaca attggctaag
    10621 gaccaaaata aaaagactga agaaaagata gctcaattaa aggaatttat agctagattt
    10681 agttctaatg cttcaaaggc taaacaagca acatcaagaa agaaacagtt agagaaatta
    10741 gaagttgaag atatacaacc atcaagaaga agatatccat atgtaggatt tacacctgca
    10801 agagaaattg ggaatgaagt tttagaagta cacaatttaa ctaaaactat agatggggtt
    10861 aaggttcttg ataatgtgtc atttagattg gatagagatg ataaagtagt atttatggga
    10921 gatgaaatag ctactactgc acttttcaac atagttatgg gagaacttga accagacagt
    10981 ggagagtata aatggggagt aactacttct caagattatc ttccaaaaaa tcataataag
    11041 ttttttgacg gagtagaata ttctttggtt gactggctta gacagttctc tgaagaaaaa
    11101 agtgaaagtt ttataagagg atttttaggt agaatgttat tctcaggtga agaagcatta
    11161 aaagaagctc aagttatatc tggaggagaa aaggttagat gtatgcttag taagctaatg
    11221 ctttcaaatg caaatgtact tgtattagat gaccctacca accacttaga cctagaaagt
    11281 ataacttctg taaataaagg acttgaaaag ttcccaggag tacttttatt cacttctcat
    11341 gaccatgagt ttatatctac tatagcaaat agaattatag agataactcc aaatggaata
    11401 atggatagaa aaatggactt tgatgagtat ttagaaagta aagagataca agaccaatta
    11461 gctaaaatgt atggaaaaga taaataatta ttagtataaa accttttaat atttgagaca
    11521 tgctatgcat gagtgtgtaa aattttaatc aaatattagg aggtttttta tgttgtttaa
    11581 acatgtttat tagcagaata gagtaatatt ttgtatatta agaatttaaa tataatatag
    11641 tagatgatat aataaaaata tataacattt tggggggaat agattatggt taagaaaagg
    11701 ttagtagctg tagtattgag tttagctatg attagtgtag ggaatttttc atatgctcac
    11761 tcaggagggc ataattcatc aggaggtcat cataataaaa ataaatcaga gttaacacgt
    11821 tattattgtg gaggacattc agcacatcaa cacaataaag gtttttgtcc ttatgctagt
    11881 acaaaagaaa attctggagt aacaatatct tcagtacata acgaaaaaaa tcataaaaac
    11941 tattttgcag aaaaagggta taataaaggt tatgaagatg ggtgtaaagg gctttataat
    12001 ttaagtgtca cagaagccaa atctgataat ttatatgaaa aagagatttt aaaaagttca
    12061 tatgaaaaag gatatttaat tggatacaat gaatataaag caaaaaaagt agatgatgta
    12121 atgaagaaga tgttagatat tccaatgtta gcaagtatat aataatatag tatttaaaaa
    12181 gatattttct ataagggaat atctttttta ttgtaaagta taatttttcg taaaaaagta
    12241 taataactga ggcgataaat taaaataaaa cagattattt aagtagttat taatttgaga
    12301 tagatacatt taatattaat gaatctatct caataagcta agctaattta aagctaagat
    12361 aaattaatat taattagcat taaataaact ttcagaaatt ttgtcagaag cctctttatc
    12421 catttcttta agtacatgag aataaacgct tagagtagta cttatatttg agtgtcctac
    12481 tctctcggat ataaccttta taggtacttt agaatttata agtaaagtaa catgtgagtg
    12541 tcttaaatca tggaatctaa tatgaggtaa ttcattcttt tctaaaaatc ttttaaattt
    12601 tttactcata acatcttcag caataggttc acatttctta ttgaaaaaca acaaattatt
    12661 attatttcta atggaagatt ttaataaatt tttattctgt tttattttat attcttttaa
    12721 taaagacatt aattctttgg gagaagatat ttttctaaca gagctttctg tctttggctc
    12781 tttgagaata acagaaccat taattcggga agttatctta tttactgtta ttgtattttc
    12841 ttcaaaatca atattatccc aagttagacc taaaacttct gaaattctaa gacctaatcc
    12901 aatagccaaa ttaataggta attccatttc tgtatttttt gctaaatcta ataattttat
    12961 catttgttct ctatcataaa tctcattttt aaatttttta tatcttggag cctctatact
    13021 atccataaca ttttctttta ttaatttgag tctataagct ctttttattg caagtttcaa
    13081 tatatttata tgaatcttaa ttgattgagg atttagttta taagatagtt tatctatgta
    13141 gttttgtatg tgaaccggat gtaagtcttg tattttatat tttccaatag atggattaat
    13201 gtaattctta caaatactaa cataattatt atatgtagct aaagatatat tttcactata
    13261 tttatttaga aaatctaata gaaattctga cactgtaatt tcgcttggta ctaaaaaacc
    13321 atcattatat aagctttctt ttacctcatt tagtctttta cttgcttccc tttttttatc
    13381 aaaagtaccc atattttttt gttttctttt acctgtcttt tcatcttcaa attccaggta
    13441 aacaacatag tttttatttc tttttctaat aaatgcattc atatataacc caccaatcaa
    13501 aattttatac atttattata acatatatat atcacaaaat aattttgaaa tagagttgca
    13561 tacattttaa tacattgtat atatttattt aaaaaatgta tatattatgt caaatattag
    13621 aattttaatt tgataatctg ttatacttat agaatagata tttatcttta tattaataga
    13681 ttgtgtgttt aattttgaat aaaaatataa attttttgta ttaataaatt tggatgagat
    13741 attttaaata aaacctttga ctcgaaaagt ctattaatat agtatttaat tatgtagaag
    13801 tttgagagga gtgtaaaatc atatgactat tactaagata ccaccagcag aattaaaagt
    13861 catgaaattt atttggagtt cagattttac agtagcatca aaagatgtca ttgaagctat
    13921 ggaaaaacta tataattgga agcaaactac aacactaaca cttttatcaa gattagtaaa
    13981 taagcaattc ttaagtgcaa gaaaaattga tagacataca cattatacag ttcttattgg
    14041 agaagaagag tatttacatt ttgaaacaaa aactttttta gataatatgc atggaagttc
    14101 tattgagagt tttatgaaat ccttgttcaa aaagggggta aatgaagaag aattgaactc
    14161 tgttgaaaaa tgggttaaag atattaaaaa agatgaataa tttggtgtag ttaattatca
    14221 taaaataaat agtttttaca ataattagtt aaatgaatca aatataaagt tttatgtaaa
    14281 atttgtgatt tttataaata tttttgtctt gttatttaat attaatacaa taagtttgat
    14341 gtaaaatcaa acctgtttga atttaattta tattgggttt gtcattaata ttattttagt
    14401 ttgatattgt atacgaattt aaaagtcaga atttttgtat attaatcatg taaattaatt
    14461 gttttagata taaagggcat aattttattt ttgtttt
//
LOCUS       DAGSWI010000102        14302 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.102, whole genome shotgun sequence.
ACCESSION   DAGSWI010000102 DAGSWI010000000
VERSION     DAGSWI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.102"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            155..1306
                     /locus_tag="MJF80_002786"
     CDS             155..1306
                     /locus_tag="MJF80_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0281436.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRSHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1424..2062
                     /locus_tag="MJF80_002787"
     CDS             1424..2062
                     /locus_tag="MJF80_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBZ0281437.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2063..3085
                     /locus_tag="MJF80_002788"
     CDS             2063..3085
                     /locus_tag="MJF80_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBZ0281438.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3075..3695
                     /locus_tag="MJF80_002789"
     CDS             3075..3695
                     /locus_tag="MJF80_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281439.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKIGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3822..4508)
                     /locus_tag="MJF80_002790"
     CDS             complement(3822..4508)
                     /locus_tag="MJF80_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBZ0281440.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4522..5067)
                     /locus_tag="MJF80_002791"
     CDS             complement(4522..5067)
                     /locus_tag="MJF80_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0281441.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5295..6542
                     /locus_tag="MJF80_002792"
     CDS             5295..6542
                     /locus_tag="MJF80_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBZ0281442.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSSGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6629..8623
                     /locus_tag="MJF80_002793"
     CDS             6629..8623
                     /locus_tag="MJF80_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBZ0281443.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDVVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8623..9945
                     /locus_tag="MJF80_002794"
     CDS             8623..9945
                     /locus_tag="MJF80_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBZ0281444.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            10224..11510
                     /locus_tag="MJF80_002795"
     CDS             10224..11510
                     /locus_tag="MJF80_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBZ0281445.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            11596..12903
                     /gene="grdB"
                     /locus_tag="MJF80_002796"
     CDS             11596..12903
                     /gene="grdB"
                     /locus_tag="MJF80_002796"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12643..12645,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBZ0281446.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            13180..13911
                     /locus_tag="MJF80_002797"
     CDS             13180..13911
                     /locus_tag="MJF80_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281447.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            13913..>14302
                     /locus_tag="MJF80_002798"
     CDS             13913..>14302
                     /locus_tag="MJF80_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0281448.1"
                     /translation="MINLFSSDLFLKYSFFFSFIVYRILMVIVQYYFLINFTNFNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIY"
BASE COUNT         5719 a         1421 c         2461 g         4701 t
ORIGIN      
        1 taactataaa ttaggtttat attttataaa aatttgctaa aaaaaacaat taaaaattgt
       61 aaagaaaaaa gatataatta gaactgtagt aataaatgtt ttattataga taatagagtt
      121 ttaagtatct taaataatat ttggaggaat atgaatggaa ttaagaccaa gaagtgcaaa
      181 attagaagaa tttgataaag taatagaatt aataaactat gtatttagaa tatcaagaag
      241 tcataaacca actatgatgg aagaatttcc tcttttacta agtaaaaata atatagaaaa
      301 tatgattata atatcagaag atgataaagt ggtttctgat gtaaactatc taattcagga
      361 tgtatctatt caaggaaaca gattgaaggt tgcagctata ggtggagtat gtactcatcc
      421 agattatgaa aagagaggct attcatctaa gatattggat aaagtagaag aaaaaatgtt
      481 ttatgatggt gtagatattg taattatatc aggaacaaga agtctataca gtagaagaaa
      541 ttgtagtttg gtaaagagtt tttataaata tacaataaaa cctgaagatg taaaaatagc
      601 atatgaaata gttgaatttg atgaaactaa ttttgaaaaa gataatgatt tagataaaat
      661 gattgaacta tataatcaaa atagtactag atttataaga acgagagatg agtttcaaaa
      721 actattgcat gcagcaacta ttgcctgggg accaattggt tacaagaagg tatttataaa
      781 ggaaaataat aatataatag gatatttgat aataagaaca ataaaaaaag aagattctac
      841 tgtaggagaa gttgctgaaa tagggttaaa ctctgtaaat gttgaaaata tattgaaata
      901 tgttgcaaat aaatttggat tagaatattt aaattataaa gtacatgtga aaaatcttaa
      961 agaccagttg aaatgtaatg ggacaaaatc tttggattat caacaaggaa ctatgaaaat
     1021 aataaatttt actaagttgt gtgatagttt aagaagttat tttagtcaat atgtagattt
     1081 tgaattacta aaatacatgg aatttaaaca agttgaaaat aaatatatta ttaaatacaa
     1141 agaagaagaa cttgtaatag aaaatttgga taagcttaat aaactgtttt ttgaaaaaaa
     1201 tgaggaacag tacaatgaat ttaaacatct aaaaaatata tatgaatttg ctacaaaggc
     1261 atttcctgta gattttccat ggactgcaaa tttaaattat caatagtttt agatgatatt
     1321 aaataaaatt gattgatacc tagagttagt ccttgttaaa taagagaaat tattggatat
     1381 tgaaaaagca attttgtaag taattagaaa ggatgagtat tatatggaca tacttgatga
     1441 tatagcagaa tgtatattaa atatgggtgt agataatata gagaatttag taaaaattgc
     1501 tatagataaa aatattgatg tggaagatat ttatgaatat ggtttaaaca aaggtatgat
     1561 aggagcttta gataagtttg agaataagga atactatctt tcagaagtta tagtatgcac
     1621 agatgcatta aataaaggta ttaatctttt gaagggaact ggaaaagtaa agaaaaaatc
     1681 aaagggagta attctgatgt ctgtagtaga aggagatact catgagatag gaaaaaatat
     1741 tgtaaaagta atggtagaag ccactggata taaggttatt gacttaggtg taaatagaaa
     1801 aagtgaagat ataatagaag aagctataaa aaacaatgta gatataatag gcttatcttc
     1861 aatgatgaca actacaatgg aaaatatgaa atcggttata gacgaactta atatgattga
     1921 gatagataaa agaccaaaag taataattgg tggaggtcct gtaagtatgg agtttgcaga
     1981 agaaatagga gcagatggat acagttcaaa tgctccaaaa gcagttaaat taataaataa
     2041 acttatagga ggtgaagttt aaatgttttc agatatgact ttacttcagt atataaatag
     2101 tgaagataga ggtttttttc tgcctgatat gggtacaaat ggattgttct taataaataa
     2161 aaaggcatat gaagtatatg atagtgtaga tttgcaatta gaattagcaa agactatgga
     2221 caagtatttt gaatcagatt ttatatactc attctgtgat gggattacat tttgtgaaac
     2281 tctgggttta gagacattaa gaccagatta tgattttcca agtgtattga cacatactat
     2341 aactgacaga gataaattaa gaaaattaga tgttccagac ccttatacga gtggacgtat
     2401 gccactcaat atagagagct tatcagttat atcaaaaagt attaaaaaac cactatatga
     2461 atcgatacaa ggtccattta cacttgcagg tcaattagct ggagctactc aacttttaag
     2521 atgtataata actgataaaa aatttgtaga agaattattg gaatttacaa ctgaattggt
     2581 aagaagatat gctgtagctg caaataaagc aggagcaaaa tatatttcaa tagcagagcc
     2641 aacatcagta accctaagta aagatagatt tgatgaattt atagtaaaaa atttaaataa
     2701 aatctacaat gaacttgatt gttggaaagg tatgcatata tgtggagaca ccagagaatt
     2761 attagataat atgctaagtt gtaatataga tgcagtaagt ttagaccaaa ttttagacta
     2821 tgaagaaatt gctcctatga ttcctagaga tatagttttg attggtaatt tagacccaat
     2881 aaaacttctt ggacgttcta agccagataa gattagaaga gagacgctaa aattgttaaa
     2941 gaagatgaga agatatgata actttttatg tgattttggt tgtaactgct taaataccac
     3001 tccagtagaa aatttacaag cagctataaa ggcaggaaga attagttata aagaattaga
     3061 taggattgat attgatgaat tataatagta ataaaaataa atatctttta aaggatataa
     3121 atttagatga tatttttata gataagaata ttgtgctaaa gtttttgggg tatggaaata
     3181 gaaaagctcc agatacaatt agtaaaatcg ttgaaagaga aataagaaat ataaatgata
     3241 ttttagatat caagatatat ataagtgaag ttgatattaa taaaacacct aaagaatgta
     3301 aaaaagcatt tgctgtacta tatacaatag gggataaaat tgattttaag atgaatgact
     3361 atatggaaaa ttgcaatatg atggcagggc tagcattgga taaaataggt atagtatgtt
     3421 tagattgtat taatgagaaa ataaaaatgt atttaaaagg aaagtatgat agtctcaaaa
     3481 tatcgcatga aatatatcca ggagataaag attttgaagt agagagacaa aaggatattt
     3541 ataactatgt aaaaaataaa tataatagta tagaaataga aatcaatgat tatcatcaac
     3601 tatcccccat aaaaagtgtt gcaatgctta ttttaatggg ggatgaagaa aatttagaat
     3661 caagatgtag taaatgtcct agaaaatgct tttagatagt tttcaagagt aagatttgtt
     3721 ttgtgtatga ataattgcaa taattttttt ggtcatttta tgtataaact aaaaaaatta
     3781 ttgcaaagta atattctatt taatttgaac agacttacaa actataatag tttctttaat
     3841 tcaataagtg tatttatttc ataagttggt atatgattag tataatttat actgttattg
     3901 gaatttaacc aacaagtatc tattcctgaa tttattccac ctaagacatc agaggttaag
     3961 ctatccccaa tcattaaggc agaagattta tcatgacaat taaacttttt gagagcgtat
     4021 ttaaatattt caggatttgg ttttgatatt ttcaactctt cagatacaac cattccatca
     4081 atatatcctt ttatttttga attttcaagt cttttctttt gaactgcaga aattccattt
     4141 gttattataa caatagtata ctttttttta agatattgta atatatcgat tgcacctgga
     4201 attaaaaatg tacattcacc taaaaaattt aagtacattt tgctaagagt ttcagaatca
     4261 acatccaaag atattttatt tgcaaaaagt tcaaatctaa gtaatttaag ttcattcaaa
     4321 gttataatat ttttttcaag gtcgagccaa agcttatcac ttatatttct atatgactct
     4381 atataattct caaaattaaa ttctaaatca aactcagata gaagtttttt aaaagcatgt
     4441 gattcacttt tcttaaaatc aaataaagta tcatctgcat caaaaaatat aaatttatat
     4501 gtcttcatgg tttttactcc tttacaagat gactgcaaaa attactttac tttcactatt
     4561 tgagtcattt tgccatttat gctttgttcc taaaggaact tttacactat ctcctttgga
     4621 caaagtaaat tcatcatcat ccataaaaag tttaacttct ccctccaaaa catatgcaat
     4681 ttcatctcca ttatgagtaa tcctatcaac acaagaagaa gtgtttggag ctaaatccat
     4741 aattgcaaat tctaaattac cactagagtc tggaattaaa atctcatata taacttcttt
     4801 gcttccaggt aaaataactt tttttctatt atcatgtctt actatcaagt tttttttatc
     4861 tacttcagat acaaaaaatg taaataaagg aacgtttaga gaagaggcta tagattttaa
     4921 tgaatttaca gatggattag caagacctcg ttctatttgg ctcaataatg atggtgtaac
     4981 atcagctagt tttgctaaat ctcgaatact taagttttgt ttttttcgaa cttcagctat
     5041 tttttcccct aaatttaaat cattcatata aatatcctct ctttttaatt taaaaaatat
     5101 taacttaaaa taatcaagta aaatgattat gtagtatgaa attattataa catatagaat
     5161 taggttttga aaaaactatg ttcaatataa ttaaataaat taaaatataa ttgacaaagt
     5221 ggatacagaa tacttaaaat atatttaaat aaaattaagc aaaagatagt taatttatga
     5281 taggagggga tattatgcaa ataacgactt ttggagctat atttggttta ttaatagcaa
     5341 ttattttaat tataaaaaaa tttcaagctg tatatagttt aatgttagga gcatttattg
     5401 gaggattagt tggaggagca aatataacac aaactgtaga ttttatggca aatggagcta
     5461 tgaatatatc accatcaatt cttagagctt tggcatcagg tgtattagct ggaagtttaa
     5521 taaaaacagg ggcagtagat aaaatatcag aacagattgt aaaaatattt ggtgaaaaga
     5581 gggctttatt ttcgattgcc atttcaacta tggtacttgc tggagtaggt gtaaatttgg
     5641 atgtatctat aataactgta gcacctatag gattatatat aggtagaaaa ttaaattatt
     5701 ctaaattgtc aatactatta gctatgttgg gagggggtaa agcaggtaat ataatatcac
     5761 caaatccaaa tactatagca gtagcagata atttttctgt caatctctct agtgttatga
     5821 tggcaaatat aattccagca attattggag ttgttattac agttatttta gcaagtatat
     5881 taataaataa aggaaacaaa gtacaatcat atgaaatttt agagcaaaga gaagatttac
     5941 caagtttatt taaatcatta tgtggtccaa ttatagctat atttctattg tttttaggaa
     6001 atgtatctcc tatagttata gacccgatga tagcccttcc aataggagga atcgtaactc
     6061 taattgtcac aggaaacttg aacaattcaa gagaatattt agcttttgga ttatctaaga
     6121 tgcaaggggt atgtatatta ttacttggaa cagggactat cgcaggaatt atacagatgt
     6181 ctgaattaca acagagtaca ataggtgctt tacaattttt aaatatgcca caatttttat
     6241 tggcaccagt ttcgggtata cttatgtcat tagcaacagc atcatcaaca gctggtgcta
     6301 ctatagcatc atctactttt catgatgcta taataaatgg tgggctatca ccgatttcag
     6361 gtgcatcaat agtaaatgct gggtctagtg tattcgagca attaccacat ggctcactat
     6421 ttcatactag ttcaggtagt ataaacatgg atataggtga aagatttaag ttaattccat
     6481 atgaagcatt gataggaata gtaatgacta taatttcaac atctattcaa ttagttttat
     6541 aataaatata aatatttaat gaaaaaagta ggattttgtt attaaatatt gaatttatta
     6601 tagtaaaaaa gaagattgga gttttaaagt gaataaaaaa aagattgtta ttatagggat
     6661 tatttattca tttttagtag tattttcact tacaaatatg tatgtaaata tggagtataa
     6721 tctaaatgta tttgaatata ttaagaagtc acttccattt acagaagaag aaaaaaagtg
     6781 gttagaaaaa cacaagaatt tgatttacag ctctgaccaa agttcacctc ctttgagata
     6841 taaaggtaag gaagatggac agtataaggg aattattgtt gatttaataa attctctgtc
     6901 aattcaaatt ggtagagatt tttattttaa accaaataat tggtggaaag aatcttttgt
     6961 aaattcaata gatgatagta taaagttttt tgacttgatt ccatcaaaag aaagggcaaa
     7021 taaatttata tttactgacc caatatacac tttgagtgca aatatattga aagataaaaa
     7081 gtcccaagat ataaatagtt acatggattt aaaggggaaa acagtagcaa ttccagaagg
     7141 tgattattct ataaattttt taaagcaaaa aattcaagat ataaacattt tgttgactcc
     7201 agatataaag actggagtca accatttaat gtctggaaaa gtagacgtag ttgttggtga
     7261 tgaaccagta ttgcgatact atataaataa ttatggactt tcaaataaat acagtgtttt
     7321 aagtaatcct atatatacta aaaaagcagt tcttgctgta cctaaacaat atgaagagct
     7381 ggttagtatt ttaaataaag gcatttttaa acttcagaaa aatggagtgt ataaagattt
     7441 aaagaaaaaa tggtattcta cgtataatga agttgatgat atattgtatg agcgaggaat
     7501 tgtgcctagt atttatcttt ttataggtat aattttaata tcaatttatg ttttttatag
     7561 ctatacatat ttgttaaaga tagaaatcaa aaggaaaaca gaacaggtaa tagaaaataa
     7621 aaaaacttta gaggcaactt ttaatagtat tacagatata ataatgcttg tagatgaaaa
     7681 taacaatata gtagaatcga acaaagtact atatgacttt atgggtgaaa tgagttataa
     7741 gatagcagat ttgattagca tgataaaagg tgttattgaa aataccttct ctgaaaatac
     7801 taataaaact agtgaaatag aaatacacaa taaaatttta aagataaaca cttttcctgt
     7861 agagtataaa aaaaataata ctgagtatat agtagttctt attaaagata taactaatga
     7921 taaaatagta gaggcgaaat tacttagaga aaacaaaatg atatctatag gacaattagc
     7981 ttctggggta gcacatgaga taagaaatcc tttgggtata ataagaaata attgctatct
     8041 cttaaaagat aacgttacaa tggaagaagt aaatgattgt gttaaatcaa tagaaagtaa
     8101 tgtagataga gcaagtaata taataaccaa cttgttaaat ttcgcaagaa tatcagatga
     8161 taatttagaa catataaata taaaaaattt tattgaaaat atagtgaaac ttcaatataa
     8221 aatgttacag ttaaagaatg ttgaaataaa aattgactgt gaacataatt taatatgcta
     8281 tataaatgga gaatcgttaa aacatgtatt tataaattta atttctaatt caatagatgc
     8341 aatacatcaa gatggaaaaa ttattatata ctgttatgaa aaaaatcatt gtttatttat
     8401 agattttaaa gataatggtg aaggaataaa agaagatgtc ctaaaggaca tatttaatcc
     8461 cttctataca acaaaaccaa ttggtgaggg aactggttta ggtttatata ttacttataa
     8521 tgagattaaa aagaataatg gagatataag tgttgaaagt aaattggggg ttggaacttg
     8581 ttttcatata aaaattccat taaataaaga ggtgactata taatgaaaat actattagtt
     8641 gatgatgagc tagaatatgg agtggtaatg aaaaaaattc tacaaaagaa gggctatctt
     8701 gtagatatag cactaagtgg tgaagaagct ataaatataa ttaaaaaaga taaaaattat
     8761 gatttggttt tatctgatgt tatgatgaaa aatatggatg gagtacagct attagataga
     8821 attaaatcta ttaatagaga tatagaggtt atattagtta caggatatgg aagtattgaa
     8881 aatgcagttg atgctatgaa aagaggagct ttatcttatt ttataaaaag taatcccatt
     8941 gaaaatttat tagaagaagt tgaaaaggta aaggcaagta aaatatctgt ttctgtacaa
     9001 aaaaataatt ttgaatttac tttggaatct aaaaatagag attttaataa tatcataaaa
     9061 atagcaaaaa aagctgcttg taaagatgtt aatatattaa tcttggggga atctggtgta
     9121 ggaaaagata tattagctag atatatacat agcataagtc caagaaagaa tgagatattt
     9181 gtacctgtaa attgttgttc tttttcagaa aatctattgg agtctgagct gtttggtcat
     9241 gaaaaaggtt cttttacagg agcagtagat agtagaaaag gtagatttga actttcgaat
     9301 aaaggaactc tatttttgga tgaaatagga gatatacctc taaatgttca ggtaaaactt
     9361 ttgaggacac ttgaggacaa atctatagag aggataggaa gtaataaatc tattaaagtt
     9421 gactttaggt taatttgtgc tatgaataaa gaaccaaaag tggaaatatc aaatggaaat
     9481 ataagagaag atttttttta tagaataagt acaataacaa taactatacc accactaaga
     9541 aagagaagag aagacttaac tactcttata gaatttttct taaataagta ccaaattgaa
     9601 catgataaaa agatacactc gattgataag gaagtaaagg attttttatt aaattacaat
     9661 tatccaggaa atataagaga attaaaaaat ataataaata gacttgtagt tttaagtgaa
     9721 gaaggtaatt tatcaaaaga caatttaaat ttaatatcaa ataatgtata catagatgat
     9781 aaaataagta ttagaccttt aagagaaata agaaaagagt ttgaatgtga gtatattgaa
     9841 aaagttttat ctttgtgtgg aaataatatt tcaaatacag ctaaaaaatt ggagattagt
     9901 agaagacagt taaccaataa aatatcagag tataatatta agtgagaaaa aaacttctca
     9961 ttaagtgaga aaaaaatttc tcaaactgta ttaaaagata aacagaaaaa atctgtttat
    10021 ctttttttat tataatttta aaaattaata tttacaataa ttattgctaa attcaatatt
    10081 ggtgtaaatt aaaaatgaaa atttctatga cattaataca tagaatatat attttaaata
    10141 attggcatag aaaatgcttt atatattaat aaacgtaata aatactttta attgcgatta
    10201 aatatattaa gggggatttt catatgaagc tagagctagg gaatatattt ataaaaaatg
    10261 ttgaatttgg agaaaagaca gaagtaaaag atggagtact atatattagt agcgaagaaa
    10321 tagaaaagat agtattagaa gatgaaagga ttgtaagtgt aaatgttgaa cttgcaagac
    10381 ctggcgaatc aataagaata gctccagtta aagatgtaat agagcctaga gttaaaattg
    10441 gagatgaaag caaaatattt cctggaatta taaataaagt gaagactgta ggaagtggaa
    10501 gaacccatgt acttttagga gcttgtgttg taacttgtgg aaatatagtt ggatttcaag
    10561 aaggcgtaat tgatatgagt ggtcctactg ctaaatatac acctttttca aaaacaaata
    10621 atatatgtat tgttataaaa gcaaaggatg gtatagatac tcatgattat gaagaagcag
    10681 ctagaattgc aggattaaaa ataggtgcat atgtaggtga ggcaggaaga gaagttgagc
    10741 cagatgaagt cattgtatat gagacaaagc cattattaaa acaagtaaaa gaatatccag
    10801 acctgccaac agtagcatat gttcacatgt tacaatcaca aggactttta catgacactt
    10861 attactatgg ggtggatgct aaacagatag tgcctacatt tatgtatcca actgaaataa
    10921 tggatggagc tataatatct ggaaactgtg tagcaccatg tgataaggtt acaacattcc
    10981 atcatttaaa taatccagtt atacatgact tatataaaag acatggaaaa gatttaaact
    11041 ttataggagt tattttgact aatgagaacg tattcttagt cgataaagag agaagctctg
    11101 atatggtagc taaattaata gaatttttag gagttgatgg agttctagtg acagaagaag
    11161 gttatgggaa tccagatact gacctaatga tgaattgtag aaaatgtagt gaagttggag
    11221 caaatgtagt tttgataacg gatgaatttc ctggaaaaga tggcaagagt caatctatag
    11281 cagacgctac taaggaagca gatgcagttg tttcttgtgg tcaaggaaat ctagtggttc
    11341 atttcccagc aatggaaaaa attataggaa ctttagatta tgttgaaatg atgataggtg
    11401 ggtataaagg atgcttaaat gaagacggaa gcatggatgc agagctacaa attataatag
    11461 catcaactat agcaaatggc tataatcatt tgacagcaag atattattaa ataagtattt
    11521 gaatataaag ttctgttgat gtaatcattg gtctaactga tgtaataact ataaagtgat
    11581 ttggaggatt aagagatgag taaaatcaga gtggttcatt atataaacaa tttctttgct
    11641 ggtataggtg gagaggaaaa agcagatatt ccgccagaaa aaagggctgg tgtagtagga
    11701 cctggaatag catttcagag tcaatttaat gatgatgcag agataatctc aactgtaatt
    11761 tgcggagata cgtattttgg tgaaaatatt gagagtgcaa ctcaagaact tttaaaaatg
    11821 ataaaagaag aaagccctga tttatttatt gcaggcccag catttaatgc aggtagatat
    11881 ggagtagctt gtgggactat ttgtaaggca gttgaagaag agttaaatat tcctgtaatt
    11941 acaggcatgt ataaagaaaa tccaggtgta gatatgttta aattagattt gcatatagtg
    12001 tctacaggaa attcagcagc aagtcttaga aaggtagttc caataatgac gaaactaggt
    12061 cttaaattgg ttagaggtga ggaaatagga cctcctgaag aggaaggata tataatgaga
    12121 ggaataagaa aaaatttctt ccatgaactt agagggtctg aaagagcaat tgatatgttg
    12181 gttaaaaaga tgaaagggga aagctttgaa actgagtatc caatgcctga atttgataga
    12241 gttacaccta tgaaacctgt taaagattta tctaagataa aacttgccct agtaacttct
    12301 ggagggatag ttccaattga taatccagac aaaattgaat cttcaagtgc tactaaatat
    12361 ggaatttacg atttaacaga tatggattct atgtcaaata aagaatttac aactatacat
    12421 ggggggtatg acagagcata tgtccttgaa aatccaaatt tagttgtacc actagatgta
    12481 gttagagagc ttgaaaaaga aggtgtaatt ggagaattag tagattattt tataactact
    12541 acaggaacag gaacgagtgt tggcaattct aagagatttg gagaagagtt ttctaagaaa
    12601 ttgttagaag atgatgtaga tgctgtaatt ttaacttcta catgaggtac ttgtactcgt
    12661 tgtggagcaa cgatggtaaa agagattgag agagctggta tagcagttgt tcatatatgt
    12721 acagtagtac caatatcact tacaatagga gctaatagaa ttgttccagc agtagggata
    12781 ccatatccac ttggaaatcc tatgttagga gaagaggaaa gtaaaaaaat tagaaagaat
    12841 atagttttaa aagctttaaa tgcactaaca gaagatatag aagaacaaac agtatttgaa
    12901 taataaaaat acaacacaac actgaaataa aaaaagatat caaaaaataa ttttttaaaa
    12961 ttattttacg atatcttttt ttattaggta aattcgaaat ttgcattttt ttaaattaaa
    13021 tttataaaag tgataaatta gtttattata atattgatac acaaattcaa attagatata
    13081 ctatgaaaag cgagattatt acattttgga acaaatattc aacatattag atcatattaa
    13141 ttgaacagaa aaaattaact ttatataata attagaataa tgataagaaa gaaggtgtat
    13201 atattgattt caattgccat ttgtgatgat gaatcctata tgctaaatca tttaaaggaa
    13261 aatatagaat tatacatgaa gcagaagaat ttattatata atatagagct ttttaataaa
    13321 gtagaagatt tactatgcaa tcatgaaaag tttaatattg tatttttaga tatacagatg
    13381 gaagagatta acggaatgca ggcagctaga aaattaaggt catatggtaa tgagtgcttt
    13441 ataattttcg ttacagtttt aaatgaattt gtatttgatg cttttgaagt agatgctata
    13501 aattatctga taaaacctct atctaatgag aaactatata aaactttgga taaaatagtt
    13561 acaaaacttg atgataatga aagtaattat atcacaatac aaaaaggtta ttcatattgg
    13621 aaattaaaac ttgctgatat tttatattgt gaagttataa aaagaaagat ttatgttcat
    13681 ttaaagaaag agactgtaga ttattatgag actattgaaa atttagagag aagtttacca
    13741 acaaattttt ttaggtgtca tcgtagttat ataatcaatt tacaatatgt atatggatat
    13801 gaaaatggta atgcagttat ggaaagtgga gatagaatac ctgtttctag actacgtcaa
    13861 caaatatttt caaaagttat gcttgagttc atgaaaaata ggaggatata agatgattaa
    13921 tttattttct tccgatttat ttttaaagta cagttttttc ttctctttta ttgtatatag
    13981 aattttaatg gtaatagtgc aatactattt tctaatcaat tttacaaatt ttaattcaaa
    14041 attttggtgt tttattttat attcgataat ttcaattttt ttaatcatta taaatacata
    14101 ttttaattta tataatttat tttcttcaca gattctattt tttcttatga cttttttatt
    14161 tgtaaagtac ataataaaac aatctactgt tttatctata gtatcaactt tgttagtata
    14221 tttaattgaa caattagtat atggatttac agctccatta aattacttga tatttactag
    14281 tgatataaat acagatattt at
//
LOCUS       DAGSWI010000103        14272 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.103, whole genome shotgun sequence.
ACCESSION   DAGSWI010000103 DAGSWI010000000
VERSION     DAGSWI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.103"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            154..618
                     /gene="bcp"
                     /locus_tag="MJF80_002799"
     CDS             154..618
                     /gene="bcp"
                     /locus_tag="MJF80_002799"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBZ0281449.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            628..1371
                     /gene="yaaA"
                     /locus_tag="MJF80_002800"
     CDS             628..1371
                     /gene="yaaA"
                     /locus_tag="MJF80_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBZ0281450.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFAIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            1499..4156
                     /locus_tag="MJF80_002801"
     CDS             1499..4156
                     /locus_tag="MJF80_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBZ0281451.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVAGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      4210..4312
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4422..5687
                     /locus_tag="MJF80_002802"
     CDS             4422..5687
                     /locus_tag="MJF80_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBZ0281452.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            5717..6607
                     /gene="metA"
                     /locus_tag="MJF80_002803"
     CDS             5717..6607
                     /gene="metA"
                     /locus_tag="MJF80_002803"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBZ0281453.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            7098..7541
                     /locus_tag="MJF80_002804"
     CDS             7098..7541
                     /locus_tag="MJF80_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBZ0281454.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            7572..9389
                     /gene="ftsH"
                     /locus_tag="MJF80_002805"
     CDS             7572..9389
                     /gene="ftsH"
                     /locus_tag="MJF80_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBZ0281455.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            9567..12269
                     /locus_tag="MJF80_002806"
     CDS             9567..12269
                     /locus_tag="MJF80_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBZ0281456.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            12303..13001
                     /locus_tag="MJF80_002807"
     CDS             12303..13001
                     /locus_tag="MJF80_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281457.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKETWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            13358..>14272
                     /locus_tag="MJF80_002808"
     CDS             13358..>14272
                     /locus_tag="MJF80_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBZ0281458.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCII"
BASE COUNT         5626 a         1607 c         2561 g         4478 t
ORIGIN      
        1 tttcagtcta ttttaacata taaatacatg aaaattatat atttatgaaa ctttatacat
       61 aaaaaataaa atattttatt tatatataga tttatagggt aataatataa gcatagtata
      121 aaattattaa taaaaacaaa aggagagata tttatgttga gtataggaac aaaagcacca
      181 gaatttacac tagaagataa agatggaaat aaggtaagta tgtcagattt taaaggcaaa
      241 aaagtagtag tatattttta tcctaaagat aatacaccag gatgtacacg tcaagcttgt
      301 gcttttagaa atgcatatga tggttttaaa aaggaagata tacaagttat tgggataagt
      361 aaagatagta taaaatcaca tcaaaaattt gcagaaaagc atgaacttcc ttttatttta
      421 ctttctgacc ctgacttagt tgctataaaa gcttttgatg tatggaaaga gaaaaaaatg
      481 tatggaaaaa ctgcattggg agttgtacgt gcgacatata ttatagatga aaatggaatt
      541 attgaaaaag tctttgaaaa agcaaaacca gacacaaatg cacaagagat tcttgaatat
      601 ttggaaaaac aggagtaata agaatttatg attataatgt tatcaccagc taaaaatatg
      661 aagaatatag aagtatttga tagagatttg agtttgcctt gttttataga taacacaaaa
      721 gaaatagtgg aaaatataaa gacatttgct atagaagatt ttaaaaataa aatgaaaatt
      781 aatgaaaaat tagctgtatt aaataaaaat agatttgaaa gcattaaatt tgatagatta
      841 ggaaaccctg caatcttaac ttatgatgga atacaatata aaaatataga agcagaaaat
      901 tttacaagga aagatgaaga gtttgcaaat agctgtattc gaatcataag tggattgtat
      961 ggtgttgtaa aaccatatga tagtatatat gaatatagat tagaaatgca gacaaaatta
     1021 cgagtaggtg agtttaagaa tttatacgaa tattggggaa atcgtatata taaagaacta
     1081 atcaaagaaa aaacagctat cgttaatctt tcatcaaatg aatacagcaa atccatagaa
     1141 aaatttatta aagatagtga tacatatatt acatgtactt ttaaagtaaa taaaaatggc
     1201 atattgaaag tagaatctac acaagcaaag aaagctagag gtatgatgac aaaatacatt
     1261 gtaaaaaata ggataagaga tattgaagaa ctgaaaaagt ttaatttgga aggttataag
     1321 tataaagaaa acttatctaa caatagtgaa tacatatttg taaaagagta gaagttttgt
     1381 taaatattaa atggcattgt taaatttagg taaaatatac ttagtgtgta ttgaattgaa
     1441 cttgatttta aaatataatt attcttaaat taaaaaaaga aaggagtggt cttccaaaat
     1501 ggaaaatcac aaacagatgc cttggcattc aagagcaacg catgaaattt tagaaagtct
     1561 agattcatca gaggaggggc tcaccgatac cgaggcggaa aaacggctta aagagaatgg
     1621 tttaaatgaa cttagaaaaa aaccacctag aacaacttta caaatgcttt ggagtcaaat
     1681 tacagatgct atggttatga tactaatagg agcagcaata ttatctttga tatttggcga
     1741 atttacagaa gcttttgtaa tactaataat tgtaacagta aatgcagtta ttggaattgt
     1801 tcaagagaaa aaagcagaat catcattaga agcattaaaa aatatgaact cacctacagc
     1861 tcgtgttatg cgtgatggtg aagaaaatgt tattccagca agtaatcttg tagcaggaga
     1921 tattgttttt ttagaagatg gagctatggt tccagcagac cttcgtttaa tagaaacatc
     1981 aagtttaaaa attcaagagg cttctttaac aggagaatct gttccatcag aaaaagatgc
     2041 agatacaatc ttacctaaag aatgtgtatt aggagaccgt agcaatatgg cgtatacatc
     2101 ttcaatagtc acatatggta gaggtgtagg tgttgtagta gcaactggta tggatacaga
     2161 agtgggtaac atagcacatc ttttagattc acaagatgat tttgatactc ctataaaacg
     2221 taaacttaat actgttggaa agacattaag tgtcataggt attattgtat gcgttgttat
     2281 atttgcaata ggtgcatttt atcagagacc tctcttgcca atgtttatga ttgcaatttc
     2341 ccttgccata tcaattattc cagagggatt acctgcaaca gctactattg taatggcttt
     2401 aggcgtacag cgtatggcta aaagaaatgc tttaattaga aaattacctg ctgttgaaac
     2461 attaggagga gctacagtaa tatgttgtga taaaactggt acgttaacct taaataaaat
     2521 gactgttaca catattgctg tcaatggaga ttttgaatct ggaaaagtga catcaataga
     2581 tactgcatct gaaaaacatc cattagtgta taaagagatt gtttatgctg gtgcactttg
     2641 taatgatgca tgtattaatc cagataaaac agatgaaata ttaggtgacc caacagaggg
     2701 cgcattaatt tttatggcca aaaaatttgg aatagatcaa gaagctcttg aagaagaata
     2761 tccacgtgta tttgaacagc cttttgattc agatagaaaa tgtatgacaa ctgttcataa
     2821 tatagatgaa aagattattg cttatactaa aggagcagta gacgaaattc ttgacctatg
     2881 tactaaaata ttaacttcaa aaggtgaaag aaacattact gaagtggata aaaagaatat
     2941 acacgaatta tgtcttaaca tgtctaaaga tgcattgcgt gtacttggtt ttgctaagcg
     3001 tgagatttca tctataccaa aagaagatag tgaaaatata gaatatgatt taacatttat
     3061 aggtatggtt ggtatgattg acccaccaag aactgaggta attgatgcag taagtacttg
     3121 taaagaagca ggtataagaa ccataatgat aacaggtgac cataaagtta ctgccacaac
     3181 aatcgctcat gagttaggaa tatggagtga agaaaatact gtgatatctg gtgatgaact
     3241 tgataatttg agtgatgatg aactagatga agctgttaaa aatactacag tatttgctcg
     3301 tgtatcccca tttgataaac ttagaattat acaatctcta aaaagaattg gagaagtacc
     3361 agcaatgact ggggatggtg taaatgactc tcctgcctta aagtctgctg atattggtat
     3421 agccatgggt ataagtggta cagatgttgc aaaagattct tctgacatga ttttaatgga
     3481 cgatagtttt acaacaattg cttatgcaat aaaagaaggt agacgtgtat atagaaacat
     3541 acaaaaagtt atacaatttt tgcttgttgg taatatagca gagatactta cgttgtttgt
     3601 agcaatatta ttaaattggg atactccgct tcttgctgtt catattctat gggtaaatct
     3661 tgcaactgct acgcttccag cccttgctct tggagttgac ccaccaagca gaaatataat
     3721 gaagcaccag ccagtgaaat caaccacttt gtttgaaaaa aatcttatac aaagagttgt
     3781 tacccaaggt atatttgtgg caataatgac tattttagca tataaaattg gtcttaatat
     3841 agaaaatcat tctgtaggtc aaacaatggc attttgtgta ttagcatttt cacagatgtt
     3901 acgagcattt agtcagcgtt caaatacaga ttcaatgttt aatagaaata atggtaaaaa
     3961 tccatttctt ttaatctcat ttttaacttc tgcattttta atagcagtaa tactatttgt
     4021 acctgttttt agaaatgctt ttaacttaac agttttaaat aacttagagt ggcttataac
     4081 gattgtttta gccataatgt ctgtaattca agtagaggtt gggaaaatta ttaaaagaaa
     4141 aaaattaaaa aaatagttga caaaattagg aaaaatatat aagatataaa acatagaaaa
     4201 attaaataaa tcttatccag agaggtggag ggactggccc tatgaaaccc ggcaaccagt
     4261 atatttatat acattggtgc taaatcctgc ggcgaaagct gagagatgag tataattaag
     4321 cttatacaga gcctgagtta tatactcagg ctttatttat tttaactctc atctggtaaa
     4381 tagtttaatt ttttttacta aaatctaagg gggatacaag aatgtataat aaagaaacaa
     4441 tatgtgtgca aggaaattat aaaccaggta atggagaacc aagagtacta cctttatatc
     4501 aaagtacaac ttttaaatat agcagtatag accaacttgc tgaattattt gatttaaaag
     4561 ttgatggaca tatatattca agaataagca atcctactat tcaagctttt gaagaaaaaa
     4621 taagtttact agagggtgga gtatcttctg tagctgtatc atcagggcaa tctgcaaata
     4681 tgttggcagt tttaaatata tgtaaatcag gagatagtat actttgttct tcaaaagtat
     4741 atggaggaac attcaattta ctaggaccta gtcttaaaaa atttggtata gatttaatat
     4801 cgtttgactt agattcaagt gaagatgaga tagtagaact tgcaaaggaa aatactaagg
     4861 ttgtgtttgc agaaacactt gcaaatccaa ctcttgaagt catagatttt gaaaaaatag
     4921 caaatgtagc taagagaatt aatgttccat ttattgttga taattcatta gcatctccag
     4981 tgctttgtaa ccctttaaag tatggagcaa atatagttac tcattctacc acaaaatatt
     5041 tagatgggca tgcttcaagt gttggaggaa ttatagtgga tggtggaaac tttaactggg
     5101 ataatggaaa atttccagaa ttagttgagc cagacccaac atatcatggt ataagctata
     5161 ctcaaaaatt tggaaatgcc gcatatgcaa ctaaagcaag agttcagttg cttagagact
     5221 atggaaattg tttaagccca ttcaatgcgt atcttactaa tttaaatgtt gaaacactac
     5281 atcttagaat ggagagacat agtgaaaatg cacttaaaat agctagattt ttagaaaaac
     5341 atgaaaatgt agattggatt aattacccag gacttgaaga taacaagtat tatgagaatg
     5401 ccaaaaagta tttatcaaga ggatgtagtg gtgttttatc atttggagta agaggtgggt
     5461 tagaaaatgc caaaaaattt gtggaaaaat tacagatagc atctttggtt acacatgttt
     5521 cagatgtaag aacttgtgtt atacatccag cttcaactac tcatagacaa ttaacagaag
     5581 aacaattaat tgcatctgga gtattgcctt cactaataag attatctgtt ggaatagaaa
     5641 atgtagagga tttaatagct gatttaaatc aagctttaaa tttctaatat tctaagagaa
     5701 agagaagtga aaagatatgg ctttaatact accaaaggga ttacctgtta ttaataaatt
     5761 attagatgaa ggaatagatg taatttataa agaagatttt aaaaaggaat taaagtatga
     5821 agaaaacata gacactaaga tagctatact aaatctaatg cctataaaga tagatacaga
     5881 attagattta ctaagaagga tagataaaac aggattcaac gtgtctgtgg aatttattaa
     5941 aatttcaaca agagaaagta aaagaagttg taatgaatat gttaaagaat tttataaaac
     6001 atttgatgaa gccaaaggag agtattttga tggctttata ataacaggag ctccagttga
     6061 acaaatggag tttgaagaag ttgattattg gaatgaacta gaagaaatta tggattattc
     6121 taagagaaaa actaaatcta cattatatat atgttgggca gctcaagcaa gtctttacaa
     6181 atactacaat gtaaaaaaac ttccactaag tcaaaaatgt tttggagtat ttaagcataa
     6241 ggtagataaa gatagtaaaa tagtagatgg atttgaaaat gaattttttg ctccacattc
     6301 aagacatact actgtaaata ttgaagcttt gaaagagaat aaagagttga gcattgtgag
     6361 tcattctaaa gaagctggtc cctatataat tacaaattca agggatgtgt tcgtaatggg
     6421 tcatagcgag tatgacaaat acactcttga taaagaatat aagagggata taaataaagg
     6481 agataaaata tctatacctc aaaattacta tattaatgat gacccttcag aagagccaac
     6541 tgtaaaatgg aaaaaacatt cagaattgtt gtttagaaat tggataaaaa actatttgat
     6601 acaataaatt ttaagagtat ataaatgtta actaaaaaaa tatagaaatt tataggtata
     6661 aagttaatct taatgatact ttatacctta tttttttaaa atttgacctt ataatagtag
     6721 ttttatattt taagaaaatc actttaggtg tatgtagata gaggtggctt taattttata
     6781 tatggtgcga taatgggaga ctaaaccttt agagtatcaa taaatttgta ttttaacttt
     6841 taaaagagat taaaaaggga gaaatcagtg taaaatatat atatcgattt tttattaaat
     6901 tttattaaaa atatcctgta taaaattaaa tatcaataag ataacaaaat tccaattgaa
     6961 gttacaatat tgtaattgac aataaatact taatactata atatttaata cattaaacat
     7021 tatagtgtta agtatttatt tacagtatgc acttcagcct aaatttgaaa taaagtattt
     7081 tcgtagggag cgtttgtatg gatgagatga gttttaagaa agagctacta gagttgacta
     7141 gagatataaa tatgaaattt acaactttat taagtgattt ttatcagcca cttggtatta
     7201 cagcagtgca agctttgatt ttatctgagt tatgtgaaca tggagagaaa aaaataagcg
     7261 atttagggaa gaacttaaac atgacaaata gtaatgtatc agtaatctgt caaaggcttg
     7321 aaaaaaatgg ctttttaaat cgcataagag atatagaaga ccaacgaatt gtaaaagtaa
     7381 aagttacaaa taaatcattg gatatacaag aacatatttc aagtagtatt tttgatagtt
     7441 attttgaaaa tatgaccagt gaaaaattgc aagatatgga agatataata gaaggtttag
     7501 agaaattaaa taaactttta acttacattg attgcaaata acataaaaat aatttaaagg
     7561 agaataaaat tatggatgaa cataaacccc aaaaaaggtc agcactctat tattatacag
     7621 ttataataat agtagtaatg tttttaaatg ctttaatatt tccaagttta atggataaaa
     7681 aaatggttga gattgactat ggaaagtttc tcacaatgtt ggatacagga aaggttaaat
     7741 ctgttgagat agaaaataat agaatagcaa taaatccatc tagtagcaag gataagaata
     7801 tatacataac tggtagaatg gatgacccag aattggtaga tagactaaaa aatgcaaaag
     7861 tagaatttac taaggttatt cctaaggaaa attcacctct tttaagtatt ttacttacat
     7921 ggataatacc tataggtatt cttatgctat ttggtaactt aatgatgaag tctatgcaaa
     7981 agcgaatggg tggtaatgct atgcaatttg gaaaaagcaa cgccaaaatg tatgtatctg
     8041 ctcaaagtgg aaaaagattt aatgatgtag ctggtcaaga tgaggcaaaa gaagctttaa
     8101 ctgaaatagt agatttctta cacaatccag aaaagtataa aaagatagga gcacaaatgc
     8161 ctaaaggagc acttttagta ggacctccag gtacaggaaa aacactcctt gcaaaagctg
     8221 tagcaggtga agcaaatgta ccatttttct caatatctgg ttctgagttt gttgagatgt
     8281 ttgtaggaat gggagcttct aggattcgtg atttatttaa acaagcaaaa gaaaaagctc
     8341 catgtatagt gtttatagat gagattgata ctattggtaa aaagcgtgat aatggaagtg
     8401 gattaggtgg aaatgatgaa cgtgaacaaa ctttaaatca gttgttaagt gagatggatg
     8461 gttttgatgg tggaattgga gtagtaatac ttgctgctac aaatagacct gacagtcttg
     8521 acaaagcatt acttcgtcca ggaagatttg atagacgtat accagttgaa cttccagacc
     8581 ttggaggacg tgaagctata ttaaaggtac atgctcaaaa tgtaaatgta agctcagata
     8641 tagattataa tgcaattgca aggtctacat caggagcatc aggtgcagaa cttgcaaata
     8701 ttgtaaacga agcagcactt ttagcagtta agtctggcag agatatagta gaacaacagg
     8761 attttgatga atctgttgaa actgtaattg ctgggtatca acgtaaagga gctgtaattt
     8821 cagataagga aaagaaaatt atagcatatc atgaaatagg tcatgcactt gttgctgcta
     8881 tgcaaaaaca ttctgcacct gtccataaga ttactataat acctagaact tcaggagcat
     8941 tgggatatac tatgcaagtt gctgaagatg agagtgtact tatgtctaaa gaagaagcaa
     9001 ttgataaaat aactactttt acaggtggtc gtgctgctga agaggttatc tttaacactt
     9061 gtacatcagg agcatcaaat gatatagaac aagctactaa aattgcaaga gctatggtta
     9121 ctcgccttgg tatgagtgaa gagtttgata tggtcgctct tgaaactgta agcaatcaat
     9181 atttaggagg agatacttct cttgcttgtt cttccgacac tgctgctaaa atagatgaag
     9241 aggtatttaa aatagtaaag gcttgtcaca aaaatgcaat tgaaatctta gaaaataata
     9301 ttgataaatt gcatgaactt gctaattatt tattagaaaa agaaactatt acaggtgatg
     9361 agtttatgga aatacttaac ttaaactaaa atgattatac acaagataaa aatgcctatt
     9421 gttaggcatt tttattttac aactaaattt tttaaattca tatttaaatc tttacataat
     9481 cttaatcatt aaaaatacat cttaactaca attttatatc ggaattgata ttataatagt
     9541 aatgaatggt gtaaggaggt tttttaatgg atagacctaa tcctgatatt ctattagaaa
     9601 aaattaaaaa tgaagaagaa aaactaagta gaggtcaatt aaaaatattc tttggttatg
     9661 cagctggagt aggaaaaacc tactctatgt tggaatctgc acaaaattta aaaaaagtgg
     9721 gtgtagatgt agtagttgga tatattgaac cacacactag accagaaaca ttggctttac
     9781 ttgatggctt ggaaattctt cctgtaaagg agatagaata taaatctata aaactaaaag
     9841 aatttgattt agatgctgct ttagcaagaa aaccagaagt tatattagta gatgaatttg
     9901 cccattctaa tgttgctggg ttaaggcata caaaaagatg gcaagatata gaagaattgc
     9961 tgttagcagg cataaatgtc tacactactg taaatgtaca acatttagaa agtctaaatg
    10021 acattgttga gataattaca aacgtatcag ttaaagagac tataccagat aagtttttgg
    10081 atacaaatac acagttagaa cttatagatg ttgaacctga tgtacttctt gaaaggttca
    10141 atgaaggtaa gatatataag aaagaacaag ctactagagc aaaaaataat ttttttataa
    10201 aagataacct ggttgcactt agagaaatcg cacttagaaa aactgcagag agagttaata
    10261 aagaagttca aatgaccaga ctatcaaagg gtgatgtaac tgtaatacct acatcagata
    10321 cacttcttgc atgtataagt ccatctccat cttcagcaaa agttattaga actgcatcca
    10381 gaatatcaga cagtacattt gcaaaatgga tagctttata tgttgaaact ccaaacacag
    10441 caagacttcc atttgatgaa caaaaacaac tacaaaataa cctaaaatta gctaagaaat
    10501 taggtggaga aataattgtt ttacatggtg aaaatataat agagcaaata cttagaattg
    10561 caaagctgag aaatgtaaca aaaattgtaa taggtagaaa tcattcaagt aataaaagat
    10621 ttagtaaaag atttaaaaaa gatatagttg acaaattaat tgatgaagtt gattatatag
    10681 atatacatat aattccatat aaatttgcct cagatgttaa atacaaacct aaaaaggata
    10741 aatcatcaat aaaatctaaa tttaaaatta gcaaagttga ttttataaaa cttatattta
    10801 ttactttgat gataagtatt ttagcatatg cagtacaaag catgggattt ataagagaaa
    10861 atatattgtt aatatatatg ttgggtgtag ttttagtttc tatgtggact aaagggtata
    10921 gtacaggtat aataagttct gtatttaata tagttctttt aaattatttt tttacagcac
    10981 cattatatac attatctata gcagattcaa actatatagt aacactggtt gtattctcaa
    11041 tagtaggtat aataactagc actcttacgt ctaagataca gcatgaagca gaaactgctg
    11101 ctaaaagaga agaaaataca aaaatgatat atcaaataat taagggattt ttaagattat
    11161 caaataaaga tgatatagta aacaaaggta tagaactttt gtctcttagc ttaagtagag
    11221 atgtaatttg ttatttaaat gtagataagc ataataaatc aaagctttac aaaaaaaata
    11281 ctaataataa ggataaaaat gatttggata gtgaagatga aaaagctgtt gcaagttggg
    11341 ttttatccaa ttcaacagtg gcaggaaatg acacggatac acttcctggt tcaaagggat
    11401 actatatacc aattgtagga atgaatttaa ctttaggagt agtgggaata tcatgtattg
    11461 attcaaaatt ggatacagaa gatataagct taattgaaac aataattgct caaatggcaa
    11521 tagcgcttga tagagaaatc ttgtcagaag ctaaagaaaa taccaatctt gagattgaaa
    11581 gggaaagatt aagaagtaat cttttaagag cagtttcgca tgaccttaga actccactag
    11641 caggaatatc tggtgcagta agtacaatta taaaaaacaa gggtactata ggtcaagata
    11701 taatagatga attacttagt ggtgtatatg aagatactca gtggttaata agacttgttg
    11761 aaaatttact tagtatgacc aagatagatg aaggaaaatt ggaagttaaa aaacattctg
    11821 aactggttga ggaaattata tcagaagcgt tacaaaaaat aaaaaaaaga attgaaaatg
    11881 cacttataga tattaatata ccagaacaga tattatttgt tcctatggat gcaaaattaa
    11941 tagaacaagt tttaattaat ttaatagata attctttgaa atattcaaag gaagattgta
    12001 agatagagat aaatgtatat gagaaagatg attatgtttg gtttgaagta tctgataatg
    12061 gtcctggtat atcaaaggaa cttaaaaagc atatctttga cagatttttt actggagaag
    12121 aaggagctaa agattccaga aagggtgttg gacttggatt atcaatttgt aaatctataa
    12181 ttcaagctca taaaggtgaa attatggtag aaaacaataa agataagggt tcaactttta
    12241 agtttagttt gcctaaggaa aatgaatagt tttaaatttt aaaattagtt ttggaggaag
    12301 aaatgagtaa aaaacttata ttgttagtag aggatgataa gacaataaga aaatttatat
    12361 ctactgcact tttaacacag gattatgatg tcaaggaggc tataactgga aaagaaggta
    12421 tatctattgc tgtctcatat tctccagatg ttgttctttt agatttaggc ttagaagata
    12481 tggatggaat agaggtaata aaagctatca gacaattttc aaatatacca ataattgtag
    12541 tttcagctag agagcaagat agagacaagg tagaagtatt tgatgcaggg gcagatgatt
    12601 acttaacaaa accattctcc atagtagagt tattagcaag agtaagagta gcatttagac
    12661 attcacaagt agaagctcaa caaaaagatg atgttaaatc tacgtttgaa gtagataaat
    12721 tattgattga ttttgataaa agaaaagtta ttgttgatga tgttgaagtc catttgacac
    12781 ctattgaata taatattctt tcattacttg caaagcatca tggaaaagtg cttactcata
    12841 attttataat aaaagaaaca tggggtagtg ttatagggaa tgagactaag tctttaaggg
    12901 tctttatggc aacgttaaga cgaaaaatag aaaaacaacc tgcaaaccca agatatataa
    12961 ttacagaagt tggtgtagga tacagattaa atgatgaata gttgtaatat attaattatt
    13021 agattatgtg cttattaata tatattaaaa ttatatagtt tagttaaaat aagaatgaag
    13081 tgtatgtgct tgttgtcagt ttagcacatg cattttattt tttagctttt ttagtatcaa
    13141 taatttttta gtatcaataa tatgaaatat gttttaaaat aagtatatag tgtaatctgt
    13201 aataaaaact ttggttaaat aataaatgta tttaacactt accttataat aaatttaata
    13261 taaatattaa ataataatta ttaaaatagt gtaaaataat tacagtaagg attataaaaa
    13321 atttttagaa tgtaaattga aatttaggag tagatgtatg tatatactga atttaaatac
    13381 aagagagact attgaagatt ttagagataa attttatgta gcagaaaatt cttatcttat
    13441 attaagtgct ccaaaaaatt taaaattgct taaggaaact ttggatatag atgaaataac
    13501 ttttaatgat tgtcttaaat ttgatgaaat aaccaaactt gatttatttg ataattacga
    13561 ttttttaagc ctaaatacat ttgaactaag agatggagaa gctgttatag aagaggtaaa
    13621 tatgtattta tcagataatt ttatattggt tgtagtgaat gaagaacact ttttatttga
    13681 atttgtaaaa aatattattt tgaagaactc tcaacttgaa aaaaatccag ttattaatct
    13741 atttaagata aactacttga ttcttagaga agttataaaa aatggttttg agtctttgga
    13801 aaaagttgaa gagttaattc ttcaaataga agatgaaatg atggacaaca ttaataagaa
    13861 tcatgtatct agaataagtg atgtaagagg attaacaaga attatagtta agaatactag
    13921 acctcttttg tatattgggg atagaatagt taaggaaaat ataagatatt taaaatattc
    13981 aaatgtaaaa aaatataatt tagaaaattt tcaaggtata gattttggaa ttgacaaatt
    14041 atacaatttc gcattgtcaa cacgagagtt agcagataaa ttattagata tttattcttc
    14101 cagagtaggt gagaaaacaa ataacttaat tacaaaatta accttgttaa cagccatatc
    14161 agcaccactt acaattataa ctggtatata tggaatgaac tttagatata tgccagaatt
    14221 aaactggatt tatggatatc cagcaacttt attttttatg ctatgtataa tt
//
LOCUS       DAGSWI010000104        14072 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.104, whole genome shotgun sequence.
ACCESSION   DAGSWI010000104 DAGSWI010000000
VERSION     DAGSWI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14072
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.104"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            402..1805
                     /locus_tag="MJF80_002809"
     CDS             402..1805
                     /locus_tag="MJF80_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBZ0281459.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2263..3285)
                     /locus_tag="MJF80_002810"
     CDS             complement(2263..3285)
                     /locus_tag="MJF80_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBZ0281460.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3320..4918)
                     /locus_tag="MJF80_002811"
     CDS             complement(3320..4918)
                     /locus_tag="MJF80_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBZ0281461.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(4960..6225)
                     /locus_tag="MJF80_002812"
     CDS             complement(4960..6225)
                     /locus_tag="MJF80_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0281462.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAE
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6367..7794)
                     /locus_tag="MJF80_002813"
     CDS             complement(6367..7794)
                     /locus_tag="MJF80_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HBZ0281463.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLFASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQIYMGVEE"
     gene            complement(7832..8839)
                     /locus_tag="MJF80_002814"
     CDS             complement(7832..8839)
                     /locus_tag="MJF80_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBZ0281464.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDK
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(9036..10358)
                     /locus_tag="MJF80_002815"
     CDS             complement(9036..10358)
                     /locus_tag="MJF80_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281465.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIGTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10716..12356)
                     /locus_tag="MJF80_002816"
     CDS             complement(10716..12356)
                     /locus_tag="MJF80_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBZ0281466.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHVSHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12461..13117)
                     /locus_tag="MJF80_002817"
     CDS             complement(12461..13117)
                     /locus_tag="MJF80_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281467.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(13143..13844)
                     /locus_tag="MJF80_002818"
     CDS             complement(13143..13844)
                     /locus_tag="MJF80_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBZ0281468.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     RKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
BASE COUNT         4780 a         2649 c         1672 g         4971 t
ORIGIN      
        1 aacaaatcct ataattatat tctaatatac tacctactac tttgtgctac aacaatttct
       61 ctatttatat gatttgtaaa atatagtaca acatttttta tcattttcat ttcaactaaa
      121 tttgtaatat tttattcaat acttaataaa tacttaatca atacatgctt tataatactt
      181 tactcaatat ataatcaata cataatttat aatttgtaat actttattca atacataagt
      241 agaaatctct atatacataa attcattttt tcatacatct tacaaattta tttttattta
      301 gataacatgt atattttttc catgaaaaat aaataataaa aaatagtaaa tatggaaaat
      361 ataaaattga ttaacaataa ttaattacct gaggtgtgaa tatgaaaatg ctaaacaact
      421 tttttaagaa aatcaacaat ccagctcctg tctttgcaca gacaaaaatg gaaagagata
      481 atcaaacaga tgattcactt tctacaaaac caaagactac atttagagac gttgctggat
      541 tagacgaagt aaaagaagaa ctatttgaaa tagtcgattt tatgaagtca ccacaaaaat
      601 atcaaaaaat gggtgcaaaa atacctaaag gagtattatt ttatggtcct ccaggaacag
      661 gtaaaaccct acttgcatct gctgtagctg gtgaaacaaa ctcatcattt ttcaatgtta
      721 ctggttcaga gttcgttgaa aaatatgtag gtgtgggagc aaaaagagtt agaactctat
      781 ttgaaaaagc tagaaaagaa gctccaagta taatatttat agacgaaata gatgctgtag
      841 gagcaaagag acatcttgaa agtaacaacg aaaaagacca aacactaaac caacttttag
      901 ttgaaatgga tggatttaat aaagattcca atgtattaat aataggtgct accaatagac
      961 ttgacttatt agatgaagct ttacttagac caggaagatt tgacagacac attcatatag
     1021 gtgcaccaaa ctatcacact agatttgaaa ttctaaaggt acatacagat gacaaaccta
     1081 tagacaaatc tgtaaatcta gaacttttag ctaaaaaaac tcatggtttc aatggggctc
     1141 atctttcaaa tatagcaaat gaagcagcta tatttgcagt tagagatgac agtgagtgta
     1201 ttacatcaga acattttgac aaggcacttg aaagagttat tgcagggcta gaatctaaaa
     1261 actctgcatt ggttgaaaaa gagaaaaaaa tagtagcata tcatgaagct ggacatgctc
     1321 ttgtaagtga tatagtagga atttgtccta tacaaaaaat atctatagtt cctagaggac
     1381 aagcattagg ttatgttctt caattacctg atgaagatag atatatttat actaaggatg
     1441 agttgattgg aaaaataaaa atattacttg ctggaaaggc ttcagaagaa ttaatattta
     1501 atcataaatc tactggtgca aaggacgact taaagaaagt cactgaaata gctaaccaaa
     1561 tggtttgtga atatggtatg agtaacttag gatttatgac tattgatgga aatgataaga
     1621 ctttcttatg tgataaggtt caaaaggaag caaatcgtat agttgaaatt tgttataaag
     1681 agactttaga gatgttaaag gataatcttg aagatttaca ttctgtttct aaattcttat
     1741 ttgaaaaaga aactatgact catgaagagt taaaagattt gataggaaaa gaagctgtaa
     1801 actaatatag ttttaaaaat ttgaggctgt ctcaaaatag agataagcct ctttttattg
     1861 ttaaagttat aaaaatacat ttgattttat acaaaaaagt gcctcataaa ctaaaaattt
     1921 cattttgaga cacttacata ttttaattac atattttaat tttgtctatt tacagaatat
     1981 tatatctaca taaaactaaa ctgctgtaaa cttaactacc tccagtaatg tttgaagtgg
     2041 tataaaagct agtagcctag tttctacact tataatttat attttacata actaaattta
     2101 gtagattttt atactcacaa tttagaacga ttgaaaactt aaaaatttct tatcagatat
     2161 ctacaactta attaggtatc tatcacttga ttaaatatct accacttaat taaatatcta
     2221 ccacttaatt aaatatctat cacttaatta aatatctacc acttaaagtg tagaacctct
     2281 ttcaatcaat tcaggtttta acatttcaac aactgactct ggattttctt catttaataa
     2341 ggacttaagt atgttaattg ttttttcaat caacaaatct agtggctgtc taatcgtcgt
     2401 taaattataa ctcttccatg aagccatttg aatatcatca tatcctatca ctgataaatc
     2461 ctctggcacc ctcaacttgt actcaaacct tgcagcatcc attacaccaa tagctatcaa
     2521 gtcacttgtg caaaaaattg ctgttggagg atttgataat gctaacattc tcttacctgc
     2581 ttcatatcca gaatcataat catagtttgc agattcttct ttaaggttat gaatcccact
     2641 ttgtcttaac ttagaataaa atccaatttt cttttccatt tcttcacttg tttctctcgt
     2701 aaattgaata tatcctatat tcttatgacc tttcttaagc agatattcag cagccattcc
     2761 cccaccttca attgggtcta catagactgt acttattttt ataccaggta taaaacgatt
     2821 aaaaagaatc acaggaatat tgttttcttc acaagtctca gccatcacct tattcatggc
     2881 cgaagctgta ataatcacag cttctacttg aaactgaatt acttttgaaa ttatactatc
     2941 tatactttgt tgtctaggaa ctttaaaaac gagacactgt ttacctatct cttgaatttt
     3001 ttctacaaat aaattgataa ttctattgta aaatggacct agactatcac caactatcaa
     3061 tcctataaca ttagtttttc cactaatcat tccccgtgct accaaattag gaacatatcc
     3121 tatttcgttt gctacacgaa aaattctttc tcttacttca ggttttacgc cctttccatt
     3181 tgcactacca aatgctcttg aaacagatga ttgagatact cctgccaatc ttgcaacatc
     3241 ttttgaggta gccgatttat tataatgaac tttcttttga ttcatttagt atttctcctt
     3301 aaatatctag tatttcttct taaaattgta tcaaaaataa tataatatat tggataatga
     3361 aaaaagctaa tacaattagt tcttgtttac ctaaagaaaa tggcactata gatactccat
     3421 ctactttatc cattccttga gaaataaggt gatttctcat accaaatgca gtgtattttt
     3481 taatatcatc tgcattatga tttttcttta aaatgacact aaaaatatat ccaaatatca
     3541 atgttattgt agcacctatt gctggattcc acaaccaact cacattatat ttattagaaa
     3601 taaaaaatcc taaaactaac cctacaataa aaccaataga tactccttta tcattagctt
     3661 tatatgtaaa cattgctagt acaaatgcac ctgtagctgg tccagcaaag tatgagatat
     3721 agttaccaac aaggtcaaga attgatttga tgcttcctcc aaatcccata ataataaaac
     3781 ctgtcataac aactcctata actacagtta taatcattgt aacttttaat gatgattctt
     3841 tgttgtcttt tttgaaataa aactcataaa tatccttcgt aaaaacagtt gtcatactgt
     3901 ttaatataga gtcgatacta gacattgctg ctgcaaacac acctgcaata ataattccaa
     3961 tggctcctac tggaagttca tttaaaataa aagtaatcat tatttcattt gaagtttcaa
     4021 atgtccttcc cccataaaaa acaaataaaa ttactcctac caatagcata aagtagtaaa
     4081 ctaaattcat aacaaatgca ctaactacaa aagaattttt tgcacatttt aaagatttag
     4141 atgttaaaac tctttgtact tgagattggt caaaacaaaa gtatcttatc cacattacag
     4201 ttccaccaat taaagtagcc caaaaagtat ttgtcactga aatatcccta ctgaaatcaa
     4261 atgtatttaa ttttccactc tgttgtgcaa tttgtaatgt atcaaagaaa cttagattca
     4321 ttccttttaa ggctacaata attactataa atacagcact tattactact atcatttgaa
     4381 tactatcagt ccatataact gccttaatcc cacctaatag agtatatata attgatacaa
     4441 gtacaactat tgggactaaa gcataaagtg accagccagt aatcatttga ataataagca
     4501 ctggaatata taccatagaa ctagcttgaa tcaatgaatt aataaaaaat tgaataatag
     4561 ttaatcctct tgaataattt cctaaacgaa gtcccatata ttcatatata gaggttattt
     4621 ttaatttata gataactggg gtggtaatgc tcagtgcaaa aaatattgct aaaggtactg
     4681 taatattaac cattactgga tacattccat ctacatatgc ccatccagga ccacctataa
     4741 agccatttgc actaatcata gtagctgcaa ctgataaagc aattggaatc catggcattg
     4801 acctacctgc caagaaataa tcttcttgat ttttattatc ttttcctgaa taataaccaa
     4861 taactaacat taatagcaaa tatcctatta taatacatac atcaattgat gaaatcataa
     4921 gccacccctt tctaagtaac aaaataaatt cctagtattt tattttttat tatttagcgt
     4981 ctccggtaag aataaccata caatagcacc tgctacaaag actgcgattg atatgtaaaa
     5041 gatagtcatc atacctaaag taccagaagc tgtaccagct atagcaggta ttgcaaacgt
     5101 agatataatc cttcctccat taaatgacat accagatgac aacgaacgat atttacttgg
     5161 gaacaactct gtgtagtatg ctccccatgc tccagaatat ccaacagcaa cacctagtaa
     5221 tactgatatc atatagaatg ttgttgtatt atctccacct aattttgtat atataaaaac
     5281 aaatactgct gctaatgcca tacctataaa aggtattttt cttccaaatt tatctgcaat
     5341 tgaaccccag aataaatatc ctactaattg accaattcct tgtaaactat atatagactc
     5401 agccactgct gctgacattc ctattcctgt cactaggtat gtagttgcat tatttgaaaa
     5461 tgctgaatat gctgtaaaat ttgcacctga taaaagaatc gttcccaatc caatccataa
     5521 gtattttccc ttatacatac ttatatagct aatcttttca ccagtttctt ttcccttttt
     5581 aactaattcc tttgtatact ccaaatcatc tggcacaaag aagattgcta agatagctcc
     5641 aactataggt ggtataatcg cagtatacat caatatttgc caatttgcat cacctaaaac
     5701 gccatatact ttcccaccaa aaatcattgc taatgaaaat aaagcagtta atatagcagc
     5761 taacttacca cgatatttac ttggaaacat atctgcaact aaaggcatag ctgttccaaa
     5821 tacgcctccc aacactactc ctgtacacaa acgtgctatt ttccataaca taatatttgt
     5881 aggagataac attggtataa atgttgtcaa tccaatcagt aaagcaacgc taattaaagt
     5941 cctcttcttc ccaatttttg tagaaatcac cccaaaaata aatgtacctg gaattgtacc
     6001 aaaagacata actgcaaata tttcttctgt ttgttttaat gaaaaaccaa atcccccatc
     6061 tgctaaagca taaatcattg ttggtttaat aaaagtaccc accataaaat tgtagcaata
     6121 aaaaaagtat attaaagcaa ttgaaaaaaa tgcaattcct ctttttaggt ttgaatagtc
     6181 ttttcctaat atgatatcat tttctatttc aaccgttgtt ttcatatttt tataattccc
     6241 ccttttaaat ttagtcataa ttttgctaaa agtttaaaca tctatttttc atatacattc
     6301 cccacttaaa ttttataaag caatacttcc ctatatattt caaaactatt tatactctaa
     6361 acagctttat tcttctactc ccatataaat ttgttttaat acatccatac catcaaaagt
     6421 tatccactcc tcctttactt taccttgtag tatatgataa tgattgattc ccattacttc
     6481 tatatgcttt ccagaaggct gtccaaaaaa tcctatacct tcatttattc cacgtaatct
     6541 ccatcttaca gatacatcat aaccttcatt tttttctctt tgattacaag ttactctttc
     6601 cacttcataa cttccatttg gaattgatgc aaataaactt acaatcattc cttgaatctc
     6661 atcatagcca tttaaatctt tatcacatat aaaatgtact actgcattat catgatagta
     6721 ttttttaact tcatttatat atttataatt ataaatacgg cttgtcattt caagcatcat
     6781 ttcgccaaca gaatcatctt ttgcttgata cttagttggc ataaattgac catccataga
     6841 ttcacagata ccataagttg attgtaaact aagcacttta gattcacttg cctttgccat
     6901 actctttgct acttcctgag ggtttaatcc taattgagta acaatccata aattatctct
     6961 aaccaaccat tcttcatgga ttctattgtt agttgtggca caatcaatta cagttctaaa
     7021 gtttattttt ctacctgttg ctggtccaaa attactatca cctaagttag ttgctgtaga
     7081 caaaacacgg tgagaagata aaaaaccatc agcaccaaag ttagaccata ttacattttg
     7141 accaattaat cttctatcag gaaacgcatg taatgtttga agagtattgg aaataacatc
     7201 tttaatacca actaaatttg aagagccaca atgcattgta acattattgt gatatgtatc
     7261 ataaataaca cctatacctt tttcttccca gatattatgt gtaatcttca taatataatc
     7321 tacaaaatct ctatacttag aatcaaatcc aggtatttcc tgttcccttt ttgtctgttt
     7381 tgaaaaatca ttgtaatcca ttactccaac aggcgttact tctaatgaat ctccatagta
     7441 aatttttgta gatgacatat tatcttcccc cttaaatata acttttttat ttttttcagt
     7501 aagagtattt gtatttaatt tatctgtatc cactgtatct atgttaactt tatttatgtc
     7561 ggatttattt gtgtttgttt tatctatatt ccctttatct atatttcctt tatctatatt
     7621 tcttttatat gtgttcgttt tattctcatt tgtttgattt tcatttatat tactaaaatt
     7681 agtttgattc attttagctt tgttttcttt aaccgtacat gattcaagcc cacttgtaga
     7741 aacttctttt tcttcctctt tacttgttgc attatttttg ttggtagatg ccactttata
     7801 tccccctttg ttttagaata cgtatgcata actaagcatt aaaaatactt ttagttctct
     7861 caagaatatc aagcccttgt tgtttcaacc aataaggaat atcaattaac acccagtttt
     7921 cttgaagttt atcgccctta cgataataaa catcaacaac ttgcatatct gctttaacct
     7981 ctccacctgg taatcctaaa aaacctccac atggtacatt agtcagatta ggccatccaa
     8041 agaaacaagc aaaattacct tctgcaaatc tacatacatg cccattaaaa gtctttcctt
     8101 ttaaattatt tctaaatggt agctggtgtt gctcttgata tctaggtata gtataagaag
     8161 caccaattcc acctggacca taccaaatca tatctttagc ccaagatttt tcaagtattt
     8221 ctggtgggca tcccatagca ccactttcat ttaattcaga taaatcatct accattttat
     8281 ttactagtgc taaagttttt actccttcaa cttcatctgc atcttcaaaa agtaatccat
     8341 tatggtcaat tggccctgga tatacaaaat atgaacctgt ttgtggtggc aatggattaa
     8401 ctcctgcttg aatcataaac ccaattatat ctacaaataa tccagtttta gcaattttac
     8461 catcttgaac acagttaaat tctgcatatc ttaaactaat cattttgcga gtatgtttaa
     8521 tgccaagcca atccttatca aaaagtccca taaaatgccc catactcatt acccattcac
     8581 tattgtcaat ctcatttgtt cctgcaatga aaatatcttg tcgtctttgc atattactca
     8641 atgatttttt taaaggcttc caaaatacat ctgctacatt ttcagtgcct tcttgttcac
     8701 gaaatggata cacacctttc cagttataat ctgatttcat atgttgcttt aagacatttt
     8761 caacctcatc cccagtagca ttttctaatg cttcaaaata atttcttaca actttttttg
     8821 aatcctgata ttttcccata attatctcct cccagattta tttaagttat aaattaactt
     8881 tctcatttta tgcatacgat ttccttttta atgatttttt agctgttttg agtttatttt
     8941 atgaatacat atgcattttt ctaaatatta acatatatct aaaaagttgt aaagaaaatg
     9001 ttatcataaa aatttaattt ttgacttata tctatttaaa acaacagaaa aggtttaaaa
     9061 cgtaatattc tactgggtct accatacttg ctcataagct gttcactcac tacctcacaa
     9121 taaccagaat cacacaatct aagtataatc ctgtccatag ttctaatact tacaccacat
     9181 atagtagcta attcctttga tgtaaattca gtttttccac tatttattat tttattgaat
     9241 actttatata cagtattagc acttagccct gtctctgtag caatttgata aaaacgttca
     9301 tcaaaactat ttttctcttt gacagtagga tttggcttta gtggtcccat aatttctcca
     9361 ttttcaaaaa ctacatatgc tgcatcattt ttttgacttt gagccatctt aatactctca
     9421 taagcgttaa atttagcttc atttgctgtt tttccataac caattcctat ataagtgttt
     9481 ataactgaaa cttcacgaat catatccaac aaataaatat ctctgtattt ctttgtttct
     9541 aactcaagta tttttttagt tgtaaaaatc ataaattcac ttctactctg ctctaccaaa
     9601 acaccatcta tacggctatt gaaatggtat agtttatcaa gaatcttaat cctttgtgaa
     9661 agataagcgt actcatcttc cttcattagt gaatattctg aaggcatgtt cagtttaata
     9721 gcaagaacaa taatctgatt ctcattgttt ttctttgcaa tataacgcat ctgaatgttt
     9781 tttatacttt gcaccattac gtcctcagaa ggcaaggcta aaactactgg aatattatct
     9841 tttttcatta tatgataggc ctcttcaaga cctgtaatac aacaacatac atcattattt
     9901 tgataattat atttatgaaa ttcaagcaca aactttatat agtcctcttc taaatacttt
     9961 tgctctgcaa aaaacatatg ttcatcctta aattcaactc caatttcttt atacaatctc
    10021 ttcatcatag ccttatcata agtacctata ctaagtctac taatatcgaa tttaagcaaa
    10081 tatgtaattt caagcaacac tctgtacaat gttgtttcat gtctaggaat ataatcagac
    10141 aatacaaaat ctttttgtct tttttcaaaa aatttaaatg gcgacttacc tgtaaaaaga
    10201 actgcatcca ctctacctct atatttttcc aataaatcag gtacttcaat atatctatca
    10261 tatctaagaa aatctgcttc aatattagga aaattctttt tacttactgc tagtatttta
    10321 tcgattacca tacctggtcc tacataaagt attttcattt cgtctctcct ttcctaatga
    10381 tactattatc tcttaaaaca ttttctaaac ctaccatgac taaaggcaca ggactcctga
    10441 ttcatagaat tttgaataat caattatacc taatataaaa agttacttta agttttttaa
    10501 ttacgactat ctgttgaaac atttttgtat ttaaattttt gtatttaaat cttattcatt
    10561 aatattaatt acattttaca attaatatat tgttttttct acttaaagtt aaattcttta
    10621 gaaaacacaa gtagagtaat ctttattaat ttaacaaaaa tagccttcct taatattcaa
    10681 agaaagacta tttttataaa agttcttaat gtattctatt ttgctatgct aagaggattt
    10741 ttaattacac gtccagcaca tacatcagtt tgctttcctc ctgccaatgc aattttacca
    10801 ttcaccaaca catattccac tcccactgta tactgtttag gattactata agtcgcttta
    10861 tcttctattg tatctgggtc aaataccaca atgtcagcaa aataattttc tttaagtaga
    10921 cccctttctt tataaatgcc taagcgtttt gctgaaagat aagttatctt tttaactgct
    10981 tcctcaaaag tcaacatacc tttttctcta acatagtaag agaaaattcg tccaaaagta
    11041 ccaaatgcac gaggatgagg ctttccaaga gaaagtaaac cttctgtaga aaggtccata
    11101 ctatcagaac ctatacatga ctttggatgg caaacaacct tatctgtatc ctcttgacct
    11161 tgtgtatgaa caactacttg aattttactt ttttcttcaa tcaaaagcct aaagaacgta
    11221 tcaaatggat tttcaccatt gattttggct acctccatta aagttttacc ctctacatat
    11281 ttatttgctt cagtcattac agaggctagt acaacaccgt tccacccacc tgctaattgg
    11341 tagaaacatt gccatccagg aatcccttcc tccaactctt ttataattct gtctctattt
    11401 tcttgtactt ctaatcgttt taccatattt tctacaccgc cttcaaaagc ccaaggaggt
    11461 aaaagtgcac ccaaagatgt cattccagct gtatatgggt aacaatcaaa agttacttcc
    11521 aaaccatctg cctcagcttc atctagcttt ttaaatacct catcaatagt tccaaaattc
    11581 ttatgaccca taacttttaa atgagaaaca tgtagaggaa ctcctgcagt cttagctata
    11641 taaattgctt catcaagagc ttccatttct tcatcacctt catctctaat atgagatata
    11701 tagtatccat tgtatggtat caattcctta cataattcag ccatttcttc tttctttgtg
    11761 tatgctcctg gtaaataaac taatcctgat gaaagagcta cacatccagc ttccatagct
    11821 tttctaactt ccaacttcat atttttaagt tgctctaaag tagctggttc ctttgaaaat
    11881 cccatttcat tgatacgtac aggtccttgt gctacataag ctacaacatt gactgctgga
    11941 gaattttcag ttaagtaatc aaagtattct tgcattgtat tccaatgaag ctctaatttt
    12001 gttggaattg taccaattat cctagttcca agataatcaa ttaactcttg cttcttctct
    12061 tctcttatcg gagcaactcc tataccacag tttgcaataa catctgttgt tacacctgta
    12121 tggatacggc ttcttgtgta tttatcatac acaagagata aatcagaatg agtatggaaa
    12181 tctataaaac caggtgatac aaattttcca gttgcgtcta tagtctcttt agcctctttt
    12241 tgacttaaat ctccaatagc catgattttg tctcctacaa tccctacatc acttttatat
    12301 cttgaatttc ctgtaccatc aacgattatt ccattgacaa ttttaacatc aaacatgctt
    12361 tttctccttt caagacttat ttatattcat atttcttaat aaacaatcct attctttttt
    12421 ctcaacaaat aattctagtc tttctcctta ataagcaatc ctattctttt gttttattgt
    12481 tatcaattac aaagaagacc cttgtgataa gtatcccacc taacacaata aacaatgtta
    12541 ataatccacc tggtatacca attttttcac caaagtacat aattgctaac gctacagcaa
    12601 tcgtacatcc accagctcga atatccttac gtgcaatatc aacataaatt gctccaaata
    12661 atgctggaag tatataatta aatgaattag taacaaattc aggtaacaaa ggaattactt
    12721 tatagcctac aatggtaaaa atggtaatca tagaagctga tacaaagatt gaacaacatg
    12781 taccaattgt tccaacaact tcaccctcat gagttcctgc ttctacacct gcaattttct
    12841 gtgccattga tgctgcaggc atacgtatat ctccaacaga acctgcaagc caacctatat
    12901 aagaacctgg agctcctagt gtaggataat aagctatagg ttgaacaatc caactaaccc
    12961 cataagtcgc agcgactaac ccccaaattt gaaatataac agaaagcatt ggcatctgac
    13021 caaagcgaat acctacatag atagcgggaa taaagtttgc aataattgca agacctatag
    13081 aaattctccc tatttttatc atctttttat catacataat tatcacctct tatccgtttt
    13141 aatcacgata atataatctg tgtaccaaac ataccaatta acatggcaat acctaaggat
    13201 aattcttgta atcgttgatt ctttttaaat aaagttgtca atactagcat aactatagct
    13261 gaaataattg ctggtgtaag ttttggtatt tcaaatgttt ttatttgatt tgaaagaagg
    13321 taagcaaata atccaactgt agctcctgca agtagttttt taacccaagt aggattatac
    13381 ttcatgttca tcttttcaac aattccacgc attctatgac ccaaaataaa tacaacaaat
    13441 agccaaccta agttatttaa agccatcccc catagtgcat acgaaaaagc ttctgctcca
    13501 aatgcagcac tacctacttc taccccagat aaattggatg taagagatat tactccaagc
    13561 tctgttctag ctgctccgac atcacagagc ctcatccaag tcgtaggagc gccaatcaca
    13621 gctaccagtg ataataatgt aataacagga gatagagaag gtccaatggc tgtaacacta
    13681 gctgagcgaa ttgctgcttt aattcctttc ctttcaatac caagatttga agcctctttt
    13741 agtgcctctt tcataaatac gattgcttga acaactgaaa taattactaa aaaactagaa
    13801 acaatccaaa gacctgggct attaatcatt tcttgaaact tcatacttta ttcctcctta
    13861 aaataaattt ttcagcattt tattgtgagc tttctttctg taatcttgtt atgtttttat
    13921 attgttttaa tttattttat ctaagatata gtatctttaa taggagatta tctgtcttaa
    13981 agtctcacta aaaacattaa tacttaaata ttttattcat aattagtaaa tatcagaaaa
    14041 ttcacatatt atttttttaa cctgtaatta ac
//
LOCUS       DAGSWI010000105        14056 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.105, whole genome shotgun sequence.
ACCESSION   DAGSWI010000105 DAGSWI010000000
VERSION     DAGSWI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14056
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.105"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            29..391
                     /locus_tag="MJF80_002819"
     CDS             29..391
                     /locus_tag="MJF80_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBZ0281469.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            827..1528
                     /locus_tag="MJF80_002820"
     CDS             827..1528
                     /locus_tag="MJF80_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBZ0281470.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1799..2617
                     /gene="larE"
                     /locus_tag="MJF80_002821"
     CDS             1799..2617
                     /gene="larE"
                     /locus_tag="MJF80_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBZ0281471.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2672..3820
                     /locus_tag="MJF80_002822"
     CDS             2672..3820
                     /locus_tag="MJF80_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBZ0281472.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRILDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            3956..5344
                     /locus_tag="MJF80_002823"
     CDS             3956..5344
                     /locus_tag="MJF80_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281473.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSIGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            5373..6071
                     /locus_tag="MJF80_002824"
     CDS             5373..6071
                     /locus_tag="MJF80_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281474.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            6074..7507
                     /locus_tag="MJF80_002825"
     CDS             6074..7507
                     /locus_tag="MJF80_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0281475.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            7529..8266
                     /gene="truA"
                     /locus_tag="MJF80_002826"
     CDS             7529..8266
                     /gene="truA"
                     /locus_tag="MJF80_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBZ0281476.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    8377..8584
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8837..10036
                     /locus_tag="MJF80_002827"
     CDS             8837..10036
                     /locus_tag="MJF80_002827"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBZ0281477.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            10283..11329
                     /locus_tag="MJF80_002828"
     CDS             10283..11329
                     /locus_tag="MJF80_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HBZ0281478.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            11437..12600
                     /locus_tag="MJF80_002829"
     CDS             11437..12600
                     /locus_tag="MJF80_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0281479.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSDYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12689..13069)
                     /locus_tag="MJF80_002830"
     CDS             complement(12689..13069)
                     /locus_tag="MJF80_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBZ0281480.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(13072..13731)
                     /locus_tag="MJF80_002831"
     CDS             complement(13072..13731)
                     /locus_tag="MJF80_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBZ0281481.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         5662 a         1376 c         2388 g         4630 t
ORIGIN      
        1 taaaaaatta gaaaggaatt tttataaaat gattaaatta tatggatata caaaatgctc
       61 tactgttaaa aaagctaaaa attggcttaa ggaaaataat ttagaatttg aagatataga
      121 tatggttcaa aaccctccta gtaaagagga gttaaaatca atttataaga ctagtggata
      181 tgatataaag aaatttttta atacaagtgg tatgaaatat agagaattgg gtcttaaaga
      241 tattgtaaaa actgaatctg atgataaact tttagaaata ttggtaagtg atggtatgct
      301 tattaaaaga ccattacttt tggatggtaa aaatgtatta ttgggcttta aagaagatgt
      361 atggaaaagt actttgctaa aagaagatta ggaagagata ggtgtgttga ttaaaattat
      421 taattaatac acctataaat ttatgcatta aatctaattt ttataaatat taattagcaa
      481 attcaaatca aaaaaattca tatcaacaaa catattactt cttattacaa attatatcat
      541 atgttttata ttaaaatatt aaaatattac aacgaattgt tacaaaatta cttattatta
      601 accatgaaca tttgttttgt gctatcatat ttatataagg gaattgtcat aaaaaatatc
      661 ttgttcatac agttttatat agaaaaaaga aatgaatctt ttctttatat aagtatattt
      721 tgtaaattgc ctctatattt atagtttaaa taatagtggt attaaaaatc tgacaacaaa
      781 aataaaactt tcaaaaaatt aaatctaaaa agaataggag ataaatatga gtattgaaga
      841 tttatatgat tttgaatgtg ttcctttgaa caatgagatt gatattgatg ttttatttga
      901 aaagatgata gaagatggaa aagaaaaaga atatacacca attatagtca ttgatgaaaa
      961 agtagggcta ttaagaaaaa atatagatat gattactaaa gagtatggtt ctcttgaaaa
     1021 ctatagagag tattgtttaa caaaatataa tgaaattgat gtaaatcaat tttttgattt
     1081 taggaagagt gagatttcag aattattaaa ggagctaatt gatttagaag atgattttta
     1141 tgattataaa cattttagac caattatgaa tatagatata tttcatgaat ataacaaagt
     1201 ttacatagct aaaattccaa caattaaacc atatgaagta tttgcataca tacctattgg
     1261 cggatttaat gattgtccta gagatgaaga acatatagct attgcaaaat attggtatga
     1321 aaagcatgga atctttccaa tcgcaattgg atgtgataca gttcaatatt ctgtaaaaaa
     1381 tttaactaaa gatggtaaaa aatttgatga cttatgtatt gaattagtat tttattgtga
     1441 ggatattatt atacaagggt atgaaacctt aaaggctctt aaggatgtat taaaacgctc
     1501 tacaatagtg cttttttggt gggattgata ttctatcatt tagatagtat taaattgtta
     1561 gtcaccaaga atccacttgc tttagttatg gggagtgtgt caaactaggt ttttatacac
     1621 agtgtagcat agtaatatgt ttaaattaca aaggttaaaa aaatacttta atatttttaa
     1681 cattataatt aatagattta aaggtaatta taatttataa aataaatact ttatatgtta
     1741 taatcattaa gaatgtaaaa aaataatata tagttttata gttcatattt ttccagatat
     1801 ggaggtaaat tttgtgaagg aaaaagaaaa attagataaa ttaaaaaaaa tgttacttga
     1861 attaggcagt gttgtagttg cttattctgg tggagtagat agtaattttt tgcttaaggt
     1921 cgcaaaagat acattaggtg aaaatgttat agctgtgact attcatgcaa tgatgcattc
     1981 aagtagggaa attgaagagg ctaaacaata tactcaaaat tttggtgtta aacatatcat
     2041 attaaatata gaaaactttg atttaaaaga atttaaagaa aatggtgttg atagatgtta
     2101 tcattgtaaa aaatatatat tctctaaaat aaaagaggta gcaaaagaac ataatataaa
     2161 gtacatagta gatggaacaa atatagatga tttaggagac tatagacctg ggcttaaggc
     2221 attaagtgaa ttaggagtaa ttagtccatt aaaagatagt agtttaaaaa aagaagaaat
     2281 taggtcgctt tcaaaaacat taggattaaa tacttttaat aaaccatcat ttgcatgtct
     2341 tgcgagtagg ataccttatg gagttgaaat aacagatgaa aatttaagaa tagtagaaaa
     2401 gagtgaagaa tatctatcca atttaggttt ttctcaattt agagtgagaa tgcatggaga
     2461 tatagctaga atagaagttg gacaggaaga actaggtaaa ttttttgaaa ataacaattt
     2521 taataaagtt gatactaaat taaaaatatt tggctttaaa tatgttacac tagatatgtc
     2581 aggatataaa atgggaagta tgaatttaaa tgtgtagatt attttaaaaa taaataagtt
     2641 ttatagttaa tataaggaaa caggaggttt tatgaaaaat tatacgttaa tatcgccatg
     2701 tttttttgga atggaaaaaa tgctggctag agagattact aatttaggat atgaaataat
     2761 aaaaacagaa gatggaagaa taacatataa aactgatgag tttggaatag caaagtcaaa
     2821 catgtggttg agatgtgctg aaagagtaca tcttaaaatt gctgaatttg aggcaaaaag
     2881 ctttgatgaa ttatttgaaa atacaaaaag aataaactgg tctagatata taccttatgg
     2941 agctcaattt cctatatcaa aagcttcctc tataaaatca aaattataca gtacaccaga
     3001 tgtacaagcc atagtaaaaa aggctatagt agaaagctta aagaaaagtt atttggagga
     3061 tggtttgctt aaagaagata aggagaaata ccctattttt gtatttatac acaaagataa
     3121 agtaaccatt tctatagata caacaggaga tgctttgcat aagagaggat atagagaaaa
     3181 ggcaaataaa gctccaataa gagagacttt agctgctggg cttatttatt taactccctg
     3241 gaaagcaggt agagttttag tagaccctat gtgtggttct ggtactatat tgattgaagc
     3301 tgctatgatt ggtataaata tggcacctgg cttaaataga gagtttatat cagaaaagtg
     3361 gagaattctt gataagaaaa tatggtggga tgtaagaaaa gatgctttta ataagataga
     3421 taatgaatct aaatttaaga tttatggata tgatatagat gaagaatcta tagatatagc
     3481 tagagaaaat gctgaaatag caggtgtaga tgaatatata gagtttaatg taggagatgc
     3541 aactcaattt aaaagtgaag atgagtttgg atttataatc actaatcctc cttacggaga
     3601 aagattagaa gataaagaca gtgttaaaca gttgtataag gagcttggtt atgcatttag
     3661 aaaattaaaa aattggtcgt attatttgat aacttcttat gaagactttg aatatgagtt
     3721 tgggcaaaaa gcagacaaaa aaagaaaatt gtataatggt atgttaaaaa ctaacttttt
     3781 ccaatatccg ggtcctaagc ctcctagaaa taataaataa gttttacatg atttatttgt
     3841 aaaatataaa attttattaa aataagtaaa acattatgat gccttgaaac gtctaattat
     3901 tgtgaggaga tttttgtgaa caaatttaaa gatttatagg attaggagga gttaaatgaa
     3961 agtgttaaag cttattactg tagtagccct gatgatgtta tttgttactg gatgtaatat
     4021 aaaaactgaa tctccagaac aattagcgaa gatacctgac tatgatgata caaaaaaagt
     4081 attgtatgat ggtattgacc agttactaaa accagattca tctatgatat tgccttctaa
     4141 tacaaaagaa gtaggaaaaa taaataaggt agatttaaac agcgatggaa ttgatgaatt
     4201 agtagtattt gaacaaaaag aagatttaag taataatgtg agtcaggtag ggtttgtaac
     4261 cttgagttac aatggagaaa aatacgtact tggagaccat tttttagaaa atggcgaatc
     4321 aatagaatat gctaattttt atgatttgga ctcagatgga tataaggaag ttatcttatt
     4381 ggttaaatcc aaagacaaaa cgaatatgca tatatataaa gtagtagata atgaaataac
     4441 aaaaatatat gacttggatg catcatggct tcaaaataaa gaagatttta atgacatgaa
     4501 agtaaaaatt ggatatatag atggagatga caagttagac atattgatgc ttcatttgaa
     4561 taataagact aatgaaatgt ttgcaagtgt tgcaaatttt gatgggaaaa tgaaaatcaa
     4621 agattctgtt aagtttgaaa atgtaaaaaa cttaagtgaa ttatatataa cattaggtaa
     4681 tgttgcttca tctattggaa attcttctgc agctataaaa ggtattgtac ttgatattcc
     4741 tatattgaaa gataataact atattacgca gatactttat atgaaagatg ggaaaattaa
     4801 caaagctttt agtgattatg ataaaactat tactaaatct tattatatac ctgtagatga
     4861 tattgacaaa gataagatta tagaaatacc tatagttgca ggcagtacag gtaataataa
     4921 aaatacttat agttctaaat catctgcaac tataagttgg tatagatgga atggaaaaga
     4981 aggtgctgac tcaagtttaa tatttacaag tcagatatat tataattata aatataattt
     5041 taaattattt attccaaata atttatttga taaaatactt gttgaacagg aatttgttgg
     5101 agaaaaagca gtatttaaat tttactattt tgactattca gaccgtaaga atttatttaa
     5161 tatagtagta gagagtaaaa ataaacttga agatagaaaa aatacaggta ctcaaaattc
     5221 tattgtactt caagagagcg atgaatatac tttcttacta gttgtaaaca atgcaaatga
     5281 aatggataag ctagatatga ctatagacgc attaaaagaa tatttctcat taatatatgg
     5341 ttaatattat actcaattgg aggttttatt ttatgaagga aaagatactt atactagaag
     5401 atgaaattgg tattagaagt tttgttagta taaacttaaa aagggaaggc tatgaaatag
     5461 ttgaggcagg tactggaaga gaagctatag aaaaaatgac aacagaaaag gatattacaa
     5521 tagctctttt agatgtaatg cttccagata taagtggtat agaagtatgt aaatttataa
     5581 gggagaattt tgaccaagta ggtataataa tgcttactgc taaagctcaa gaagatgata
     5641 aaatagaagg atttatctca ggagcagatg attacatcat aaaaccattt agtataaaag
     5701 agcttttagt tagagtttct gctcttctta gaagagtagc aaaggatgat tcaagtgtta
     5761 agtcgagtga gatagtttct ccaccattta tacttgatat agacaaaaga aaattattta
     5821 aaaatggaaa ggaaattgag cttacaccaa ctgaattttc aatagtgaaa tatctaatat
     5881 caaatgctaa gcagtcgtta agtagagacc agatattaga cgaagtatgg ggaactaact
     5941 atttatatga ttttaaaata gtagatgtaa atataagaag gataagaaat aaaatagaag
     6001 atgacccatc aaaaccaaaa tatatacaaa ctatttgggg ttatggatac tgttttagaa
     6061 aggaagaata atagtgttac attttgaagg tctgagaaaa aaagtaataa aaaactattt
     6121 tataataatt attataatgg ttactctgtt tgaaggattg ttcatgtttt acatacaaaa
     6181 ctactattat gattctgtca aacagctact tgaatctgaa ataaaatatg ctgatgaata
     6241 taatgctatt acaatggaaa ctacaagttt tgagaaaaaa gtaaaaaata tatttgataa
     6301 acaaccctta actaaaaatt cagaatttgg aatttcaata atagataaag ataaaaatat
     6361 tatattagac caatatggtt ttaaaagtaa agaaaaagca aattatgaag atgttaacaa
     6421 tgcattaaaa gatataaaaa ctaaaaattt aacaccttat acttatagaa taccagatac
     6481 aggggagcat gttatgagta tctccttacc acttaaggta aataatataa tagaaggtgt
     6541 tgttagatat acagtatcat tagatgctat tgataatgcg atattaaagc aagctacatg
     6601 gcttatatta gcaggaatat ttatacttat tatagctata ctaataagtc ttaaatttgc
     6661 tgagactctt ataaaaccat tacgagagct aaagaagttt gcaaatgaat tggctgtagg
     6721 aaattataat ataaaattag aaaagatgaa gattgtagat gatgaaattg gtgatttagc
     6781 acagacattt gaacacatgg cacatgagat tgacaaaagt gaaaaattaa aagaggaatt
     6841 tatatcatct gtatctcatg aactaaggac tcctcttaca tctataaagg ggtggagtga
     6901 aaccttaggt tatgaaagta taacaagaga agaactagac ttaggtttag gcattataca
     6961 agatgaaaca gaaagactta taaaactagt tgaagaatta ttagactttt caaggctttc
     7021 ttcagacagg ataaaacttc atgttgatat agttgatgtt gaaggtttaa ttgtaggtgt
     7081 tgtaaatcaa cttaaagtta aagcagctga aaaagatata tcacttttat ttgaatttga
     7141 aaatgaattt atagagaaca ttcaaggaga taaaaataga ttaagacagg ttctcataaa
     7201 tttaatacaa aattcattta aatttacgag tcaaggtggt tacataaaag ttgtagcttc
     7261 tcaagatgaa gagataacaa ccatatcagt agaggataat ggttcaggta tagaaaagca
     7321 aaatttaaat aaagtgttag ataaattttt ccaagaagat tataataagg ctggtagtgg
     7381 attaggacta gcaattagta atgaaattgt aaaacttcat ggtggaagaa tgaaaataga
     7441 aagtgaaaag aatgttggaa caaaaataac ttttaatata aaaaataaat ttgctaaaca
     7501 agcataaatt cttagaaaga ggattaagat gagaaacata aaaatgatag tagcatacga
     7561 tggttcaaga tataaaggat atcaaaaatt aggtgacaat aatatgacta ttcaagaaaa
     7621 acttgaaaat gttttaagta aaatgactaa tgaaacagtg gagataatag gttctggaag
     7681 aactgatatg ggagctcatg caagaggaca agtggtaaat tttagaacta attgtatgga
     7741 ttctttggat aaaattcaaa aatatttata tgaatattta ccagaagata tagttgtaaa
     7801 aactgttgaa gaagttgatg aaagatttca ttcaagatac aatgtaaaat caaagactta
     7861 tatgtataaa attgacaata ataagtatca taatcctttt ataagaaaat atgctactca
     7921 tgtaagtaaa aaattagatt tagatagaat gagaaaagca agtgagtatt tagtaggaga
     7981 acatgatttt actagttttg cttcttctaa atctaagaaa aaatcaaatg taagagaaat
     8041 ttattctata aatataaaag aagatgataa tgttatagaa atatatgtag aaggaaatgg
     8101 atttttgtat aacatggtaa ggataatagt aggtgctttg atagatgttg gtctaaaaag
     8161 aagagctcca caagatatta agtatatgct tgaatcaaaa gatagatgtc aatcatcaga
     8221 tactgcacca gctaaaggac tatgtctttg gaaagtaaca tattagtcaa ttatataaaa
     8281 ctctctttaa aataaaaata aaataaatta tatagttgac ataaatggaa aaataaacta
     8341 ttatatattt tataagagag atataaaact ttatatttaa atcctgtgag aaagagagta
     8401 acttctatgt gttttataga gagctgagga tggtggaagc ttagcaaata agtattagtg
     8461 aaaagaactt tggaggatac taattgaatt tagtatgtta gtaggtaatc tcgccttaag
     8521 agaaagagtg gataagttta tcttatcaat tagagtggta acgcggatat atttcgtctc
     8581 ttggtttttc caagaggcgt tttttaatat atttaattat tagtgtttat ctggtatgtt
     8641 gaacatttac taattgatta gctaagttta tcaataagag tggtaccgcg gatatttcgc
     8701 ctcttagtat tttaaaatac taaggggttt ttttagtatt aaaatgattt tatgtgagtc
     8761 taaattctca gagtcattca atattaagaa tttctataaa ataaaaatta taatttaaga
     8821 ttgggaggaa ttaaaaatga aggaaaaagt agtattagca tattcaggag gacttgacac
     8881 atcaatcata ataccttggt taaaggaaaa ttatgaagat atagatgtta tagctgtttg
     8941 tggaaatgta ggtcaggaag ataaaatgga agatgtatat gaaaaagctt tacaaagtgg
     9001 agcatcaaag gcatatgtgg atgatataag tgaagaattt gtaactgaaa ctatatttaa
     9061 agccgtaaaa gcagaggcta aatatgaagg aaaatactta ttaggaactt ctttagcaag
     9121 acctataata gctaaaaaat tagttgaagt tgctcataaa gaaggtgcaa aatatatatg
     9181 ccatggatgt acaggaaaag gtaatgacca agttagattt gaagctacaa ttgcagctct
     9241 tgacccgact ataaaagtta ttgcaccttg gagaatttgg gatataaaat caagagaaga
     9301 tgctatagac tatgctgaaa aacataatat aaaggtaaca gcaactaaag ctaaaatata
     9361 ttcagtagat gctaaccttt ggcatgtttc aacagaaggt ggagatatag agcacctaga
     9421 aaatgaacat aaaaaagatg tttataaaca atgtgtagac ccagaagatg catgtgatgt
     9481 agctgaatat gtagaagttt actttgaaaa aggtgtacct aaaaaggtaa atggcgaaga
     9541 attatctcca gttgcactga ttcataagtt aaatgagttg ggttgtaaac atggaattgg
     9601 tgttatagat atagttgaga atagacttgt tggtatgaaa tcaagaggta tatatgagac
     9661 tccaggagga acaatccttt atgaagcaca taatatttta gaaagtgcta ctttagacaa
     9721 agatacatta catttcaaac aaatggtatc ttataaatac ggagaactta tttacaatgg
     9781 actatggtat tgtaaattaa gagaatctat agatgcattt atggaacaaa ctcaagataa
     9841 tgttacagga acagtaaaag ttaaacttta caaaggaaat ataaaacctg ctggaatatt
     9901 tactgaaaat gctctttatg atgaaggaat atcttctttt ggaaatagtg aactttatga
     9961 ccacaaagat gcagaaggat ttattaattt attcacttta ccattaaaaa tcagagctat
    10021 gaaagctgga aaataataaa ataaattatt taagagtgta taaaaactat ttttaggttt
    10081 ttatatgctc tttttttatt tgaatttatt aaattataca gtaaatagta taaaaaaata
    10141 tattatagtt ccaaatggct taataatgtg aaaatataag acctaaaata taaattttat
    10201 aaatacaata gttattgact ctctcattgt aagagggttt atactgaatc taatgaaaat
    10261 ttatagtgag gagatagatt atatgagaaa aataattgaa attaaacatc attcacatga
    10321 ttatgagtgt atgtggaatg gtatagaaga tttatatata aataaaaccg gtgaatgcct
    10381 tccaaacgat tttttctttt tactgtcagg ttttggttca ttttgttata tgaaaacaaa
    10441 taaagctgat ttgaaaagaa tggtagcttt gggagatgga aggacaaaac agatgtataa
    10501 atttttagca ccaattgttg gatttgaata caaacatcat gagtttaaaa agtatgagca
    10561 agcattaaag aaagcaaaat ctgaaattga tgatggatat ccagttattt taggagcttt
    10621 agatatgtat tatctaccat attataagaa gctatatcat aaagaacata ttccttttca
    10681 ttatatatta atggtaggat atgatgatga aggaatatat ctgtatgatt gtggtagaac
    10741 agaattatta catctttctt atgataaatt acgtgaaagc atgaattgca attcccctgg
    10801 attaagcaaa gaaaatacta tttgcacaat aagaatgaat acttcaaaga ataaatataa
    10861 cattgctaga gaagctattg ctataaagcg tgatatgttt ttaaatccac ctaaaggatt
    10921 tttgggatat aaaggttttg aaaagtttat taaagagtta ccacagtgga acactgaatt
    10981 ggataaagat gattatgata agattttgac caatatggtt atgttttttg gaacagtacc
    11041 aacaattcca aatgcaatca aaggaattaa agaaccagat actgttcaat ttaaaggtgg
    11101 atttgataaa atgagtagaa tgctcaacag tattggggag gaatacaaaa acgactcttt
    11161 tatacaagta gctgatattt ttgataaaac tgctattata attgagaaaa tatccaatat
    11221 tataatagat tacttaactc aaacatgtga tgatacagaa caacttccgt tattgttctc
    11281 tgaggtctta gagcatatga aaacaggata tttaatcttg gatgtataaa aaatatattg
    11341 ttgaccctaa cattacgtaa tagtgtaata tgaaaatata gcttgaatat attagtaggt
    11401 attgatggct gtaaaattaa aatttgaggt gtggatatga gaacagtgaa acaagtttct
    11461 gatttaactg gaataagtgt gagggcatta cattattatg atgaaatagg tcttttaaaa
    11521 ccaaataaaa ttacagatgc aggatataga ctttatgatg atgaatctat taaaactctt
    11581 caacaaattt tattttttaa ggagattgat attcctttaa gagaggttaa ggaaataatg
    11641 tcaagccaat attttgataa ggttgaagct ctaaaaaatc aaaaaaaatt acttatttta
    11701 aaacgaaaga gactggatga attgatagag cttataaatc aaactctaag aggagaagga
    11761 aatattgatt ttaaagagtt tgatatgagc gagtattttg atgttttaga agaatttaaa
    11821 agagagcata ggaataaggt aattaaaatt tatggtagtg tagaaaaata caatgaatat
    11881 atagaaagag ttaagtctaa tgaagagaaa attgctaaaa tggctataaa acaatatgga
    11941 actattgaga agtttgctaa agctataaaa acaaatttta gcagtgatat attgaattta
    12001 ggagaaaaat ttgatagata taaaaatgat tgtttaaaag aaaaacatcc aaaattaaaa
    12061 gaattatata gaaagcttgt agaagattta agtaggaatc cttcttcaac agaccttcaa
    12121 gaaatagcaa aagagataac agatatatct aaaaaagatt atgaaatttt ctctatggat
    12181 actggtgatg acaactggta ttatatggtt caaaattatc tagtaaatcc tatgtggata
    12241 gaagaagtag ataaaaagta tggaagtgga gcaggaaagt ttattggaca agtactaaag
    12301 acttatctaa gagatagaaa accaaagata aatacattgt atgaaaaact tgtagaagat
    12361 ttgagtagag attgttcttc aagggaagtt caaagcattg ttgaagaaat agacaatgaa
    12421 atgaaaagaa gtaatgaatt ttataaaata gacaatggtg aaagatactt tgattatatg
    12481 tcagagcttt atttacaaga ttctgactat ataaaagtaa ctgataagaa ttatggtgat
    12541 ggtgcatcta agtttattgg agaagctttt aagatttatt ttgataataa taattgttga
    12601 aaatttgaaa gtaaaaaata tgcaagtttt ataaaagcac aaaaataagg atgtagatta
    12661 gctccatcta tatccttatt taaagttatt aaaatatata gtttacatca aacttaacac
    12721 tttcaataat atttcccaaa tatgttttta tactttcaac ttcttctaaa gattttaatt
    12781 tattttcagt ataaattgct ttgtccagaa tattaaacct taatcctatt tcatcaaagt
    12841 actcatgatt taaaactttg taccaatggt ttttttcttt attccaactc gtagatattg
    12901 aatcagcttc tttttgagct ttttcccaat cttcattttc tatagatttt tctaaaacag
    12961 atatattgtc tttatatcct tctgtaaaat catatagttt attgttaaca taaaatccaa
    13021 ataatacaaa taagatagtc catagtataa caaatgttgt agatttcaat attaatcacc
    13081 ctttttattt tgaataaata ttttgtcatt ttcatctaaa acacaaataa gaacatcttt
    13141 aaaagtttct atgtgattag attttaaaat acccattaac cagttagtat ctttttgagc
    13201 tatgtctaga tttttattta ttattctacc atccagtata agtgggatag gtaaatgatt
    13261 aaaagctctt ggaggagtac tattgtagct tgatgctggt acaacactta gattaccatc
    13321 agtctccaaa atagcatatt gtacatcttt taggttaaag tatccttgaa tcctaagttg
    13381 ttctaataat tcatctatac taattctttc ttttttaagc tctttatata caatttttcc
    13441 tttatctatt aatacagaag gtttacctga taaaaatgaa cttaattttc tactttttaa
    13501 tgataagaat gaaattagag tctgcatgat tacaagtcca gatattgctg caacaccatt
    13561 tatgattggt atattattat tttccattgg gacagatgca acttctgcaa ttaatagtgt
    13621 tataactaaa tcaaaacagt tcatttcagc tatttcacct tttcccatga ctctaagtgc
    13681 aattagcact gttatgtata atataatact tcttattaat actactgtca tatctatcaa
    13741 ttactccttt aaaaaatatt tacacttagt tagtatatcc tatataaaaa atttaattca
    13801 aagtataaaa aaccttaaat aattggattc aactatttaa ggtttttttg ataattttaa
    13861 tattaaattt tttatggatt ttctgttact tttatttatt ttaataacag attcaacaaa
    13921 actcttttta tctcctaatt ttaagataaa atcaggatat tcttcaaatt cattgagttt
    13981 tattaatttg aaagaagaag atagattttt aaagttaata gttttagagt tattcactaa
    14041 tacacttctt ttttta
//
LOCUS       DAGSWI010000106        13894 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.106, whole genome shotgun sequence.
ACCESSION   DAGSWI010000106 DAGSWI010000000
VERSION     DAGSWI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.106"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(134..967)
                     /locus_tag="MJF80_002832"
     CDS             complement(134..967)
                     /locus_tag="MJF80_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBZ0281482.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCGSNSGDSGDSSKVSVSGSTSI
                     GPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSAGLK
                     ETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREEGSG
                     TRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSLKIN
                     DVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFIKVN
                     Q"
     gene            complement(1219..3003)
                     /gene="pepF"
                     /locus_tag="MJF80_002833"
     CDS             complement(1219..3003)
                     /gene="pepF"
                     /locus_tag="MJF80_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBZ0281483.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KIIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3069..3734)
                     /locus_tag="MJF80_002834"
     CDS             complement(3069..3734)
                     /locus_tag="MJF80_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBZ0281484.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(3885..4046)
                     /locus_tag="MJF80_002835"
     CDS             complement(3885..4046)
                     /locus_tag="MJF80_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBZ0281485.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4124..4633)
                     /locus_tag="MJF80_002836"
     CDS             complement(4124..4633)
                     /locus_tag="MJF80_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBZ0281486.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            4839..5057
                     /locus_tag="MJF80_002837"
     CDS             4839..5057
                     /locus_tag="MJF80_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBZ0281487.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5075..7021
                     /gene="feoB"
                     /locus_tag="MJF80_002838"
     CDS             5075..7021
                     /gene="feoB"
                     /locus_tag="MJF80_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBZ0281488.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(7200..8255)
                     /locus_tag="MJF80_002839"
     CDS             complement(7200..8255)
                     /locus_tag="MJF80_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBZ0281489.1"
                     /translation="MTIQDYMLETAVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(8303..9103)
                     /locus_tag="MJF80_002840"
     CDS             complement(8303..9103)
                     /locus_tag="MJF80_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBZ0281490.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(9096..9887)
                     /locus_tag="MJF80_002841"
     CDS             complement(9096..9887)
                     /locus_tag="MJF80_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBZ0281491.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(9927..10349)
                     /locus_tag="MJF80_002842"
     CDS             complement(9927..10349)
                     /locus_tag="MJF80_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBZ0281492.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(10365..10844)
                     /locus_tag="MJF80_002843"
     CDS             complement(10365..10844)
                     /locus_tag="MJF80_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0281493.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(10976..13732)
                     /locus_tag="MJF80_002844"
     CDS             complement(10976..13732)
                     /locus_tag="MJF80_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0281494.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIIIKISTLITNRLDMYLNNVDIIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         4491 a         2410 c         1590 g         5403 t
ORIGIN      
        1 taaaatacct attttatcta aagatattta tatttctaca ttcaataata gacaataccc
       61 tgcaagttaa agcatcattt ttatgagctt atctcacagg gtattatatg tttactttat
      121 attataactc ttgttattga ttaactttaa taaatccttt ttctgaaaca agtgcttgtc
      181 cttcatcact taatatataa tctattaaag ctttaccttt ttctgacaaa ccatcttctt
      241 tattaactaa tataaatggt cttgatattg gatatttatt agctattaca tttgcatcag
      301 ttgcttcaac atcatttatt tttaaactat ttactttctc atcaacatat ccaaatgata
      361 tatatcctat agcattttca tttccttcaa ctgtagtttt tatatttcct gaaccatcac
      421 ttatttgtgc atctttagtt agttcttctg aatcaaaccc tacaatttct tggaatccat
      481 ctctagttcc tgaaccttct tctcttgata ctattactat tggtgcatca tttcctccta
      541 cttctttcca gttagtaatc tttcctgtat atataccttt aacttggtcc attgtcagat
      601 tttttactga gtttgcagta ttagttatca atgctatacc atctattgct attttagttt
      661 cctttaatcc agcacttttt tcttcatctt ttaaatctct tgaactcatt cctatttcag
      721 atgttccttc tatagcattt tttatacctc cagaagaacc aatctgttgt acttctacac
      781 taacaccttg atttttttct tgaaactttt ctccgatagt ttccataaga ggacctattg
      841 aagttgaacc tgaaacagaa actttgcttg aatctcctga atctcctgag ttagagccac
      901 atcctactgc taaagctcca actaacattg tactagctaa aagaacgcct attttctttt
      961 taaacatttt acttaccccc gcttatattt taattttttg ttgtcatttt tctatatttt
     1021 aataatacag attctttgtt aaatgaatat tagagcaggg ttaagtaaat gtaaatattg
     1081 ttaaatacat gtaaaaactt aattttatat gatttattct taattagatt aactttttaa
     1141 agttacttta ggcagatata aacataatac aactcatatt atcagaagtc ttcattgaag
     1201 ctatcaacct atcacatcct ataattcttt ttctagttta ttaactaatt cagcaaatac
     1261 atttaaggct tcctctatag attctttttt cgtcatatct acaccagcag attttaattg
     1321 ttctagtggg tattctgacc ctccactttt taagaagttt atatatctat ctactgcagt
     1381 acttccttct gaaagtatct gttgacttaa agcacttgca gcagaaaacc ctgtggcata
     1441 cttatataca tagaagtttg aataaaaatg aggtattctc gcccattcta ttcctatttg
     1501 tttatctact attgcagatt ttccataata cttttcattt aatttgtagt atatattagt
     1561 aaattcatca gcagtaagtg gttctccact ctctaccctt tgatgtacaa ttttttcaaa
     1621 ttcagcaaac atagtttgtc tatgaactgt agttctaaat tgttctaaat aataatttaa
     1681 taagtatata gtttcatcct tagacttaga attttctaat aaataattta taagaagtaa
     1741 ttcatttaat gtagatgcaa cttctgcaac gaatatctta tagtccgaat ataaaaatgg
     1801 ttgacttgtt cttgaataat gactgtgaac agagtgccct aattcatgaa ttaaagtgaa
     1861 tagagaatta aggtcatttt tatagctcat taatatataa ggatgtgaat cataacttcc
     1921 ccaagaataa gctcctcctt ttttaccttc attatcatat acatcaatcc acccttcatc
     1981 aaaagcactt tttatgatct tcaagtattc ttctcctaaa ggtttaagtg cctctaatat
     2041 gatatcctgt gctttatcat atggtatatc catgtcaaat ttatctgtca aaggaacata
     2101 caaatcatac atgtgtattt cattcaatcc taaaaatttc tttttaagct caacatattt
     2161 atttagatta ggaatacttt tatctatagc tgatataaga ttattgtaaa catctaaact
     2221 tacatcatct gaaaataaag acgcttctat tgctgaatta tactttctag tttttgcgta
     2281 aaatatctct gattttattc caccataaag cgttgaggca aatgtatttt tatatttctc
     2341 atatgttgaa aattcacctt caaaagcgtc ttttctaact ctactattct tactttttat
     2401 aaataaagag tagtttgaat gagaaagctt aaccttattc ccctcctcat cttctatctc
     2461 aggaaattcc atatctgcat atgctaacat atcgtataca ttttctggaa ttgtcgataa
     2521 ttctgatgta gctgcaagta tttcttcctc tcgttcactc aaagtatgtg gtttatctct
     2581 caatatatcc tttatatatt ttgcataaaa ggatagcttc tcatctttta agtattcact
     2641 taacttattc tcttctatcg ccagtatttc tggaactata taagaagtag ccacacctaa
     2701 atcagtagat aacatttctg tcttagtggc tcttccttga ttatcattaa ttctagtatc
     2761 ttcatgttgt ttcatatgag tatatacata taaattttga acaatcctag atgcattttc
     2821 agagatattt aatactttat ataagttctc cttactctca gaaagcttcc ctttatattc
     2881 ttttacttct tggataagat tttttacttt acttatgtct ttttcaattt cctctttact
     2941 ggagtacatc ttatctatct tccatttgta cttttcttct attgtacttc tatcagttcc
     3001 catatcatac acctctattt taatttgttt tatattttat tcactttaca aaatatattg
     3061 tatatatttt agtccaaata atctacattt attactcctt ctatcatatt tatttctttc
     3121 ataacgtcgt gtaagtgtat tctcttagca taagatacaa aaaaattaac cttccagtat
     3181 tcttcattac gacattctat ttccatattt cttacgataa gttgattttt ttcaaattta
     3241 tcatataaaa aaggtataga gtccaaattg cttaatttta atatcaacac attcttttct
     3301 tggctaaata aaaacgtatt gattatccta agcacaaata atgctatcat tacaaataat
     3361 ccacctagaa ttgccaattc ataataacca tatccaactg ctataccaat acatgcagtt
     3421 gcccacatcc cagcagctgt agtaagccct tttactccac ctttattttt taaaattgct
     3481 cctgcaccta aaaaaccaat tccagataat acttgagcag gcattcttgc tgggtcagca
     3541 tttgtataca aattataatc aaagaataat tgaatcgaag ttattgaaac aatacaagaa
     3601 cctattgcaa ttagtatatg tgtcctaaat cctgcaaact gtcttatttt ttctctctct
     3661 aagcctatta ttcctcctat tattagtgct aatagtaacc ttattgctat ctccttactt
     3721 cctatatcaa tcataatata aaaccctcct gacttaatta caacttatgt cctacagata
     3781 atttacacat cagaaatact attttatatc ttaatattat tattaatata tgtaaaatat
     3841 agccatctgt taatttttga tacaaaaaaa cccacaactg tgggctattt gcttaggtac
     3901 atgtatttat ttattaatat atctattttc ttacttgatt ctactaactc ttcttgaaat
     3961 atttctggat attctatgta tgtatttatc tcttccctaa ctgcttcaat ctcatttttt
     4021 aacgcttcta attcattgtt cgccataata atcaccacta ttacaaattt gtatattttt
     4081 atatacattt tatcaaacaa tattatattt tgtcaatatt atgtcaataa aacttactat
     4141 aaaaatgctt taatttaggt attgtatacc tggatactaa gcctacaaac ataccagtta
     4201 ctaatgatgc cattaacata aatggtaagt accctatagc aagtatattt tctatgatta
     4261 aacttgcaac tattaattga cctatattat gccctattgc acctataatg cttatcccta
     4321 ttagtgataa atcttttatt ttactcgcta aatacataaa taaaagactc agataagctc
     4381 ctcctatact aaatagtaga tatgataaag ggcctgcaaa tattgatgac aaaattaccc
     4441 tgatagtaac aattataaca gtatctttga cacctaacaa cattaatgaa attaaagtta
     4501 taatattgct caaacccaat tttgcccctg gaaatataac tataagagga tttggtatat
     4561 atgtctctaa tacatataaa gctaaactat accctatcat aagacctaaa aatgtcattt
     4621 ttctagtttt cattaagtta actcctccaa attttatcta tcatcttaca ttataataca
     4681 aaatatataa aaacacctaa tttagactaa aatattttta atctatttag aataaatatt
     4741 agttatttca atgtgcaatt acctctataa atcttgactt tttgtatcat acttgttaca
     4801 attttaatga taatgaattt cattaggagg tgttaataat gactgtttac gatttaaaaa
     4861 taggagaaaa aggatttatt aatagtatag atggagatat taaattatct aaaagattgc
     4921 ttgcacttgg atgtataaat agtactgaaa tagaagttaa aaaggttgcc ccacttggag
     4981 acccaattat aattagattt agaggctttg accttgcgat tagaaaatct gatgctaaaa
     5041 atatttcttt acaataatag aggaggagct acccgtgatt aatgtagcat tattaggaaa
     5101 cccaaatgtc ggtaaaacta ctgtttttaa tctacttaca ggttcaaatc aatatgtagg
     5161 taactggcct ggtgttacta tagaaaaaaa agaaggattt cttggaaaag agataaaggt
     5221 tgtcgattta cctggtatat atgcaatgga tactttctca aatgaagaaa aggtttctaa
     5281 atcatattta gaaaatgaag atgttgatgt aatagtaaat gtagttgatg cttctaattt
     5341 atcaagaaac ctttacttaa caactcaact tatgcaattt aacaagccta tagttatatt
     5401 gcttaatatg ttagacattg cagaatctaa aggagttaat atagatgcca aaaaattaag
     5461 tgaagaactt ggagtaattg ttgttccaat tatagcaaaa aagaaaaatg gtgttgatag
     5521 gatagaagaa gagattaagg gagctacgca aaaagctttt atatatcaga aaaactttgg
     5581 aagtgaaaca gaaacatata aaaaattaga atccatttta tcaagatgta caaaaactac
     5641 atatacacaa aagaaatcta tcagtgataa gatagacaat atagtcttga atcctatatt
     5701 agcatatcca atatttattg gtgtattgtt cctcttattt aaatttacct ttgactgggt
     5761 cggaggtcca ctacaagagg gatttgctag tcttatagag gcttatattt ctgcacctgt
     5821 tagtgatatg ttatctaact ctagcccatg gtttaaatca cttatagttg atggaataat
     5881 tggaggtgtt ggtggtactt taccattctt ccctttaata tttacattat tctttggtat
     5941 atcgttgttt gaagatagtg gatatatgtc aagaacagca tttttaatgg ataaagttat
     6001 gagaaaggtt ggtctatctg gtaaagcttt tatacctata gttatgggta tgggatgttc
     6061 ttctccagct ataatggcaa ctagaacatt ggaaagcgaa aaagatagaa gagttacagc
     6121 tcttattgca ccacttatga cttgtggagc aaagctacct atatatgcac tatttgtagc
     6181 tatattcttt ccacataatg ctgctttagt aactacatca ctgtatttgg tgggtatagt
     6241 tatggcaatt gtagttgcat tattcttaaa taaaacagcc tttaaaacag aggctgaacc
     6301 atttatatta gaattaccag aatatagaat ccctactata gatgcactta ttaaaaatac
     6361 ttggaataaa tcaaaaggtt tcttgataag agttgttact gtcatgtttg caatgtcagt
     6421 cgtaatttgg ggactttctt cattcaatgt ttttggatat acagaagata taaatgtaag
     6481 cttcttggct actctaggtc atattatatc tcctatattc aaacctcttg gttttgatga
     6541 ttggagaact tcagttgcta ttttaagtgg tcttggagca aaagaaatcg tagtaaactc
     6601 tttaaatata ctatatggta atctaactgt tgtcttacca acaatattta atggagttac
     6661 tgcatataca ttcttgatat ttactgcttt atatactcca tgtatagctg cacttgcaac
     6721 tatgaaaaaa gaatatggca ataaaatgat gtttacatca tttgcatatc aatttatact
     6781 agcatggata atggctttta tagttaaaag tataggtgga gtactattta tgggtaattc
     6841 aataatagaa gctttgatag gtggaataat tatagtcctt gcactcttta ttctgtttaa
     6901 taaattcaag tctacaaaaa acggaaatgt ctgtagtgga tgttctattt gttctggatg
     6961 tccaagtgct aattcttgct caagtaagga agacagcaat aaaaaaaata atgcacacta
     7021 atatataaat attttagttt aaaataagat aaaagtgggt gtctcaaaat taacttttta
     7081 gttcatgagg cactcttttt tatagaaaat caaatatatt ttcatcattt caacagtaaa
     7141 aaagagctta tctagtaaaa aagaggctta tctctatttt gagacaacct ctttttttgt
     7201 tacttattca gtttcttgct acctaacttc atgtggaacc tagtatcttt tggaacactt
     7261 gggtctacac ctcttaatgt aggtatttcc ccacatatta gttgtatagg tactataaaa
     7321 ttaaatgttt gatagtaagg attatctgtg atatcaatct ttaaattttt atcattttct
     7381 gtaacatctc tacctatagc aaatacattt tccgtccaac cagaaagtac atcaatcatc
     7441 ttagttactc taggttcttt tcctgtatct agtataaata tagtagaatc actatttata
     7501 gcattatata taccatgtat aaactcttca aactcatacc cagttacagg tattctcatt
     7561 gtttccaata attttaatgc tgcttctaat gtatctccat atatatcaga gtgacctatt
     7621 attcttattt ctttagaatt aactaacttt tctttgtttc tttcaatcca ttgtttagag
     7681 agcttataca ctgcttcaaa tctattaatt gcgtctaata tcttatttat ttcctcattg
     7741 tacttttcac tagatattat acctttttct ctagctattt gaagacctaa taacattaag
     7801 tttaactttg tacaataata tccttttgtt ttagctccag atttttcttc accacaattt
     7861 actgttaata tataatcaga aatctcatct attaaagcat tcttacaacc tgccatagat
     7921 gctattttac agcccttatc ttcagctaac ttcatagcgt tgtatgtaga ataactactc
     7981 ccaccttgag atacacctac tactagggta ttttcattat caaacttgaa tgtatcttca
     8041 gttatcataa atggatacat tttcacaaca tctatatcta ataaattttg taaatatggt
     8101 tgtacttgca ctcctgagtg atagctagta cctgaacctg ttattataat cttttttaga
     8161 tttgttctct taacttcatt aaataagcta tctgcattag atataatttc tctcattcta
     8221 actgctgttt ctaacatata atcttgtatt gtcattttat acaccttcct tatctatata
     8281 ttcaataaat tataattaat ttctataaca aacctatagc atgtaaagcg attcctgtta
     8341 ctatagtgcc tatagttaat tttgttacac ttacctttct ctttaacatg tagtatatta
     8401 caaatgcaaa ggttaaaggt aacatattag gtattatttt atctagtata tcttgtaaaa
     8461 ttacttctgc accatttaag ttgaatttca gaggagtagt tatactcaac atagttgcaa
     8521 ccattcctcc tacaacgaat aaaccaacag ttgtagccat agacattatt ttctccataa
     8581 gtccctctct ttgtattctt tcaagagaat ttacccctac ttgataacca tacttaagtc
     8641 caaatattct aagggcaaat gctggtatat tgtataatag taagaataat agtggtccaa
     8701 agatattacc ttcttttgca agtgatactc ctactcctgc tgctataatc ctaaatgttc
     8761 cccagaataa tgaatcacca attcctgcaa gaggtcccat aagagctgtc ttaacagcac
     8821 taattgaact ttcatcaaat tctggattat tagcattctc ttcttccata gctgctgtta
     8881 tacctaatat agcaggaact actactgtcg ttgtattaaa aatctccaaa tgcctttcta
     8941 ttgcttttgc ttgattttct ttcttattgt atagcttctt tattgcaggt aacattgaat
     9001 aacagtaacc taagttttgc attctttcgt aattaaatgc accttgaaga gcaaatgaac
     9061 gccaaaacat acttcttaat tctttttttg ttaatttatt caaagtcaat ctctccttcc
     9121 gttaaagtat tgattgctaa ttgaggattt tgattatttt ctttgttatt gatataaaag
     9181 tttagtacaa atgctacaca tactccaaat aaagctatag cagttatatc taatccacca
     9241 taagatgcta acaaaaaccc tataaagaaa aatacagaca tcttttttga aagtaacata
     9301 tccataagca atgcaaaacc tactgctggc aacaatgttc ctgcaacacc cagtccgtct
     9361 aatataactt taggtattgc atttatcaat gctgtaactt tatcagcacc aatcataatt
     9421 gctaaaaagg ttggaactga tgtacttaaa aagaataata ttactggcaa ccacatcatt
     9481 atggtaactt tcttaaaatc agcttgttta gcatagaaaa ctgccttttg agaaaagtac
     9541 gaatttatta ttctaactaa tactccaaga gattgtgcta agactgctac tggtactgct
     9601 actgctaaag caacttctgc accattacct gataatatag caaaagctgt acctaataca
     9661 cctcctgtta ctacatctgg aggagtagct gcacctattc ctgcaattcc catccaaatt
     9721 aattcaagct gtgcaccaat cataattcct gattttatgt ctcctaaaac taatcctact
     9781 aacatacctg taactattgg cctatcaagc atagattgac caaatatacg cccatctagt
     9841 ataccaactc ctgctataat agctaacata gtagcttgta ctaacatatt gaacctccat
     9901 tttaacttag tttaatttac attaaattac attacatttt tgattttgtt ttgattttct
     9961 aataactcat ttacataaac aattccattt tttgcttcct ctaatcctga tttaactatt
    10021 agttttgtat cctcctcaac taataaaagt atagataaaa gcattggtaa atttactcct
    10081 gtcaataatt caaatttgaa cccttgtaaa agtaagtttg tacatatgtt tgccacactt
    10141 ccaccaaaaa catctgttaa gacaacaaat tcttcactat ctttattatt tgataatata
    10201 cttctaattt ctttctcagc atcatctgct gatttatccg ttgtcataca aaaatactca
    10261 atattagtta atgaaccaat tattaattta cttgtgtcaa caagctctct ggacaattct
    10321 ccatgtgttg caattagaaa ttttttcatt ctctttctcc taaattattt tattaaatct
    10381 gatagttgtt tctttttgtc tctaggtaac attctaaatt ctatatcaat ccctaaatca
    10441 cctatttgtt taaagttatt tatatcatca tcatcaacag caactaaatc tgtaagtttc
    10501 ttcttccctt cctcatatct taatcctcct acatttattt cttttaatac tcctcctgaa
    10561 tttttagcta caactaaagc atctttggta ttttttacta acactaatac attgcttttc
    10621 tttgaagaag caaatttttc tactatttct ccagattcct ctacccccct tatatataaa
    10681 gttacattgc taggtttcgc taagttcagt gacatagctt tcatttggtc attttttgct
    10741 tcatcatttg caactagtat tacatttata cctagtgatg ttgcccatcc atatgcaacc
    10801 tgaccatgta tcaatctgtc atcaattctc atcaaagata ccattttccc caaatcctcc
    10861 atcatacatt aaaattttta ttgtttatac tctcatttat ctataaaagc aaaaagtatg
    10921 ccaaatactg gcatactttt ttaaatttca ttcacttatt ttgtaatctt gtattttact
    10981 gtggtagacc atagataata ttataaatat atcctatctc tgaaattggt atttgcacac
    11041 tataactctt ttctagcaca ctaaatgcat tttttattag ttttacttca ctatgatggt
    11101 ttttgattaa caaatccata tcagtatact cttcaatttc agtttgtcta actaatcttt
    11161 caatcataca acttaagtga acatataaag aaatcttttt atcatttgaa agttttttct
    11221 tttgaatatt ttctatagca ttaattcctt cttctattct ctctattatc ttttttgaat
    11281 ctaaaatagt tagactatct attactctaa ttaatgaaaa attctttaca atagaatcat
    11341 ttatgttctt tattttttca ttttctacta ctctgctaaa tattctaaaa acatcatctt
    11401 ctccttttcc tgatattata tcctctaata atataaaatc taccccattc acatttggat
    11461 tggcagttcc tactatagcc atcacatcat acatttcaaa aactggttct ttatctccca
    11521 tagaaacaag actatcataa tcataaggta ttattttgac atcaactatt ccaattaaac
    11581 tttcggaaag tagatgttgg atttttatag cagtacctat ccctgtaaaa caagatgata
    11641 ttatagcata ttctttattt ttttgaggat aatacaattg tatattaggt ttagtttctt
    11701 catccataat ttctgcaatc tcttcaatgt ttttaccttg cattaataaa tttcctactt
    11761 ctagtgcaga aatagtggat atttggtcta taaataacat tgggctattt atcttattct
    11821 ttaaatcttc atatataaga ttaagagagc ccatatcaaa taacacaatt aaaccattct
    11881 ttatcctatc tccctctagg tatctagaca tttgttttga tacatcagca atagtagcat
    11941 ctataggcat atcaatagct gtaaatatat ttttacccaa catccgatta catacattcg
    12001 ccatactact ggcagtagca tatccatgtg ctataatagt cgcattaact tgattaaatt
    12061 tcattcttat aattgttgat ttaataaata taatcattat gattatatct acattattaa
    12121 gatacatatc caatctattc gttattaatg ttgatatttt aattattata gagtattctt
    12181 tgtaactctc tttttttaaa tatattagta atttattttc taaagcttta tactcatcag
    12241 acaattgata agattcgtcc ttcaaataaa gataagacga aaatgccaat acattattcc
    12301 catcatattt tatattgtta ttaacttcta aatttctaaa tatatcctgc attatcgata
    12361 ttataagctc cataagtacg ttattatttt catcattatt tgaaaagact aaattgtcca
    12421 ttagtctaat cattagatta gaactctttt tactaaattt ttcctttgtt atttcatttt
    12481 tttctagtct atcaaagtaa tctaaacatt ttatgaaact ttgatagatt atatttcttt
    12541 tatttcttcc atttgacatt tcaatactaa attgtttagg agaaaagata atgcttttgc
    12601 ttttaccact ttggaattga aatatatcta cattttctat ataaagattt ctaggaaggt
    12661 cttgaagagt aacaaaaata tttccatttc ttctttgatt ttttgcataa gctcgtccac
    12721 atgcatattt tattacattt ttacattcac ctatattccc cttgaatttt atctttaaaa
    12781 tcgcatttaa tgtaatttga gttatctcta taggcaaacc aagtacctta ctttcttcat
    12841 aaataaaatg ttctacaatc tttctttttt caatagtccc tctttcttct atattgggaa
    12901 cataaatatt tatgggtatt cttcttaaaa aggtttgtaa aaattcagat agactttctg
    12961 ttgtagcaaa tattaatctt accctagctt ttctaattac tccattctct ccaatcctag
    13021 aaaacgtacc tgtatccata aatgtaaata acttttcctg accttcacta tttaatctat
    13081 gtacttcatc taaaaataaa acaccaccat ctgattgttc tattagacct gctctagatt
    13141 catttgctcc agtatatgct ccttttacat gcccaaataa tagactagac aataactcca
    13201 aattattata atactgcgca cagtttaaca cattgtatgg agaatttttt tctaatacat
    13261 tactgcttac actatatttg tatattatct tggctaaaaa agtttttcct actccacttt
    13321 cacctgtcag cattattgta agtccgccat ttggataaag tacagcggtt tttatttgtt
    13381 cgattacttc ttttaagcta ttatcatatc ctatgatact attaaacaca gtctcttgct
    13441 ttaataattt acactcattt ttaagttcat ctaaattttc ataaatactt ttttttgtag
    13501 gtcctactac atcatttatt ttatttttag gtaaaaaaat aacaggtcta ctgtttatct
    13561 ttataagatt atcagatttt actaaatcat ttaataatct acttgattga gttctactta
    13621 aattaaactt ttttactata tcttctgtta ttatgccact ttctttttta agttcatcaa
    13681 agttgttaaa taatacacat tcatttacaa aacttaaaat ttcatccctc atgttcaacc
    13741 tccaatacaa atatcaataa tactataata ttagtatatc atttatatat ttatgaaaaa
    13801 gataattata ttcctatctt gattctcttt tgtaagattg taaaatcaaa ctttttataa
    13861 gtataaaatc tagatttttt aacataatct caag
//
LOCUS       DAGSWI010000107        13886 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.107, whole genome shotgun sequence.
ACCESSION   DAGSWI010000107 DAGSWI010000000
VERSION     DAGSWI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13886
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.107"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            105..245
                     /locus_tag="MJF80_002845"
     CDS             105..245
                     /locus_tag="MJF80_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281495.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(420..740)
                     /locus_tag="MJF80_002846"
     CDS             complement(420..740)
                     /locus_tag="MJF80_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBZ0281496.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(860..1597)
                     /locus_tag="MJF80_002847"
     CDS             complement(860..1597)
                     /locus_tag="MJF80_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBZ0281497.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1812..3635
                     /gene="acd"
                     /locus_tag="MJF80_002848"
     CDS             1812..3635
                     /gene="acd"
                     /locus_tag="MJF80_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBZ0281498.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3850..8148
                     /locus_tag="MJF80_002849"
     CDS             3850..8148
                     /locus_tag="MJF80_002849"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBZ0281499.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIAQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8352..8813
                     /locus_tag="MJF80_002850"
     CDS             8352..8813
                     /locus_tag="MJF80_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBZ0281500.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8843..9997
                     /gene="nusA"
                     /locus_tag="MJF80_002851"
     CDS             8843..9997
                     /gene="nusA"
                     /locus_tag="MJF80_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBZ0281501.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10004..10285
                     /locus_tag="MJF80_002852"
     CDS             10004..10285
                     /locus_tag="MJF80_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBZ0281502.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10275..10586
                     /locus_tag="MJF80_002853"
     CDS             10275..10586
                     /locus_tag="MJF80_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBZ0281503.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10608..12548
                     /gene="infB"
                     /locus_tag="MJF80_002854"
     CDS             10608..12548
                     /gene="infB"
                     /locus_tag="MJF80_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBZ0281504.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12591..12971
                     /gene="rbfA"
                     /locus_tag="MJF80_002855"
     CDS             12591..12971
                     /gene="rbfA"
                     /locus_tag="MJF80_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBZ0281505.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            12952..13629
                     /locus_tag="MJF80_002856"
                     /pseudo
     CDS             12952..13629
                     /locus_tag="MJF80_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
BASE COUNT         5554 a         1584 c         2607 g         4141 t
ORIGIN      
        1 ataaaaaaaa ttttatttat tagagttttt ggaaataata ataacagata ttataattaa
       61 ttaacagttt ttgtaagtat ttgacaatag agagggtgta acaaatggga ataatagagt
      121 cagcatcaaa attggctgag atggttcatt tattggctgt tgaaaagggg ataactgata
      181 tagaagcatg ggatgaagct gtgaaagaat acagcaaaat ttatgaagaa agaagaaatg
      241 aatagaatga taaaaaatga aaacttgatg ataaaaaata actttattta aagaaattca
      301 atttttatag aagtattaaa aataatataa ataaaaagat ggtatttcaa atgtaagtaa
      361 aaaatcattt tgggatacca tccttattat ttattttaat aatatataag taataaatct
      421 tacttatcca tattttttat gtaacctctt ttttctagct tttttatatg ctccttacgt
      481 atttcatcaa gattaatacc atattgttct tctagcttaa acatcatagt aacacatgtc
      541 tgcgctacat ccattagttc cttagcagtt ttctttataa tatcaacttc atctaaatca
      601 ttattttctc cacttagatt tctgaattta cctatacatt cagctaattc accttgttct
      661 tcacccattt tgataaatgt agattctagt gttgggtcta aattattaag ctctggtaaa
      721 gatatagttt ttagtttcat aaaagctcct ttcaagttat tcgtatttat actaaaacat
      781 cataacaaaa ataatactaa aaaaaaactt taaattcaac ataaaaactt acatatataa
      841 taaaaatcgt gaaataaatc tatatttgtc ttaatacagc ctcatataaa acttctccaa
      901 taggtttatt tattacaaga gaagctgact tatcatagac tgtatttgct ttattaataa
      961 gagcaatata atcacctttg taataatcta taaaacttgc tgctgggtac acagctaatg
     1021 atgtacctcc tataatcaat aaatcagcat tacttatagc agaaattgtt ttagtaatga
     1081 catcactgtc aagagcttcc tcataaagta ctacatctgg ttttactata gaaccacagt
     1141 tgtcacagta aggaatatta cctcccagat ttaacataga ttctaagtca aaaaatttac
     1201 cacatttagt acaataattt ctatgtacag aaccatgaag ttctagcaca tttttacttc
     1261 cagccatttg atgaagtcca tcaatatttt gagttatgac agctttaagc ttacccattt
     1321 cttcgagttt tgctaaagct atatgagcat tattgggttt tgcattagga tatattaatt
     1381 tatccttata aaattcaaaa aattcttctg gatatctaac aaaaaaagta tgtgaaacta
     1441 attgttcagc agtaaattgt ttattgagtt tttggctaaa taatccagta gaacttctaa
     1501 agtctggaat attagattca gttgaaacac ctgctccacc aaaaaataca atattattgt
     1561 gattagctat taagtctttt aagctatttg catccatagt tacacctcca aattattata
     1621 tataaattgt actacatatt aggtcgttta tgttttatta atttaaataa atatatagtt
     1681 taataagtta aataaaggga atataaataa aatgtataca gagtataaat agacaatatt
     1741 tactaaaata acaagttgtc gtataattat attaaaattg ttttagtgta taagaagggc
     1801 gggggaagta cgtgaagaaa aaagcagctt tagcaacatt ggctatgtta cccttaggtg
     1861 tggtaaatgc acatgctgat ggagatatag gtatagtgac tataaattat ttaaatgtaa
     1921 gaaatgaacc aactgccgaa agtagcatag cctttgttgc taaaaaagat gataaagttt
     1981 tgattaaaga ctcttctaat ggatggtata aaataaaagc tgaatctgga caagaaggtt
     2041 gggcttcatc aaaatatata gcaaaatcaa atagtgactc tttaagaacg tctacaaata
     2101 aagaaaaaca agttatttca aatagtttaa atatgagaaa tggagcaggt acaagttaca
     2161 gagtaataac tgtactaaaa aaaggtcaaa aagtagaagt aatatcagag agtaatggat
     2221 ggtctaaaat caaatatgat ggaagactag gatatgtatc tagctcttat ttaggagacg
     2281 tttcaaactc aactaataaa tcaaaaacta aacaagtgaa tacaacttca ctaaatgtaa
     2341 ggagtggacc aaatacaagt tatggtttgt taggcaagtt gccaaaagga agtaaagtag
     2401 aagtaatatc agaaagtaat ggatggtcaa aaataaagta taatggaaaa gatgcctatg
     2461 tatctagtat gtacttatcg gatgtaagtc aaagtaattc agacaattct agtcaaagca
     2521 atgataaaaa gaatactgat aaggttgtaa atacagcttc tttaaatgta agaagtggac
     2581 caggttctac atatagtaag ttgggaaaag tttataaagg aagtaaagta actgtactat
     2641 cagaaagtag tggatgggct aagattaatt ttaacaataa agaagcattt gtagtaggca
     2701 attatttatc tacttcagca gatacttcaa ataataactc aaatagtaac tctgataaca
     2761 gttctaatag taatggcaat aattcttcat catcaggtca agtaaatggt atgtcaggta
     2821 taagtggggc taaaattgat tataaatctt tgagttatac tttagaatct catataagta
     2881 aacaagttga gaaagcagca tcaggaggaa atgtgatagc tccaagtaat aggaaaagca
     2941 ctccaagtcc tgaatttagt actttttcag ctcaaagaac aagctcattt gtaaatgcaa
     3001 gttcaagtga tatagaatat tatttaaatc ctaaaaattt tacaaatact actaaaggta
     3061 tgatgcagtt tttaaagatt aacagttata gagatggtat ttcagaatca agtcttaact
     3121 cgtatcttaa tggtttatct tctagtgtat ttaaaaacca gggagcagcc tttataaatg
     3181 ctgccaagaa gtataatata gatgttgtat atctagtatc tcatgctatg tgggaaactg
     3241 cttatggtaa gtctacactt gcccaaggtc aaacattaac atcttataag ggacaacctc
     3301 ttagtaagcc agttaaagta tacaattttt ttggtatagg agcaatagat aaaagcgcta
     3361 atgtttcagg tgcagaagca gcgtattcca atggctggac tagtgtagaa gctacaatag
     3421 atggctctgc aaagtggata tcacaaaatt acgtaaatag ttctaagtac aatcagaata
     3481 ctatctacaa gatgaagtgg aattatgatt atacatggca tcagtatgca actgatgtaa
     3541 actgggctaa tggaatttct ggaattatgg aaaatttaat tggtctttat ggaggaggaa
     3601 gtagtttggt ttttgaagtt cctcaatata agtaatacat aaataaaaaa tatagactat
     3661 gggtagtttt cattttattt aaattatcta tagtcttttt gtatataatt tataatctgt
     3721 aaatttaatg ttaacaaaac ttttatgtta taatattaat tctaagtaaa tataagtgag
     3781 taataggtag aaatagataa aaagtggtaa taatatatat aaagataatt ttgagaaagg
     3841 aggatatata tggaaagtat taaggaatat ttggataagt tagaaattaa taatagtggt
     3901 cttggaaagc aacttaaaga agtttatata aatagagtag tttattttaa ggaagataag
     3961 atagtttatt tttatctaac ttctaaagat attgtttctc atgaactttt agataaattt
     4021 aaagaagaat tgatgtataa actcgattat tttaaagata tgagaatgaa gataagattt
     4081 actggtcttg aaagaaaatc caataaagat gttataaaaa aatattggaa taatatattg
     4141 tatatattaa agtatctttg tccatctatt gcagggtggt ataaacaagt agaatttctt
     4201 tgtttagaag aagaattaaa aataaaactt ccaaaaggta tattttatga gcgattaatg
     4261 aaaaaaaatg taacttatgt acttaaaacc gtgcttagcg aagagttggg attagattta
     4321 aatataacaa tagaaaaagc tgttgatgaa aaggtaaata aagaaagact cataagaatc
     4381 aatgatagag aaatggaaga aaaaattagg gctttagaaa ttggaaaagt taataactgt
     4441 gaagaaaatg aagaggaaag ctatgtcatt aagtctgatg tagatgaaaa tttaatctat
     4501 ggagataatg caaatgctat gattgaaaat atagttgaat taaatgcatc ttctggaaca
     4561 gttgcagttg ttggagatat atttgatgtt gacactaaag aacttaaaaa cggaaaaata
     4621 cttatgatag cttcaattac agattatact agctcaataa gctgtaaact ttttttaaca
     4681 gatacaaata aggatggagt acttgaaaat gttaaaaaag gagcatactt aaaaatcaag
     4741 ggtgacatag tttatgatac atatcaaaga gaaattagta tgatgattag tgggataaga
     4801 aaagaaacta gaatagagag agaagataaa tcagaggaaa aaagagtaga actccatgca
     4861 catacacaaa tgtcttctat ggatgctatt tgttctgtta aaaagcttgt agagcgtgca
     4921 gccaaatggg gtcattctgc tattgctata acagaccatg gtgttgtaca aggatttcca
     4981 gatgctatga atgcaggtaa gtcaaataat ataaaaatac tttatggagt agaagggtac
     5041 ttggttgaag atgattctct cattatagag gatgcaaatg ataaagaact ttcgcagaca
     5101 tttgttgtat ttgacataga gacaactggt ttttctaata caaatgataa aataactgag
     5161 attggtgctg taaaaataga aaactttaag attgtagata gatttagtga gttaataaat
     5221 cctgaaaaag atatttctta taagatacag gaattgacag gaataactaa tgaattaatt
     5281 aaagacaagc caaccattga agaagttctt cctaagttta tggaatttgt tggagatagt
     5341 gtattagtag cacataatgc agagtttgat accggattta tatcgcaaaa atgtagggaa
     5401 caaggcttgt tatataataa caaaaaagta gatacactta tgttagcaag agtaatgttg
     5461 ccaaacttaa aaagacatag attaaatgtc gttgcaaaag aacttggtat tcctctttta
     5521 aatcaccata gagcagtgga tgatgctgag gctacagcac ttatatttaa taagtttttg
     5581 cagatgctta cagaaaaagg tgcaaaaact ctaagtgatg taaacaatat acttggaaag
     5641 attgattaca caaagcttag cacaaatcat ataaccttga tagcaaaaaa ttctataggt
     5701 ataaaaaatt tatataaaat aatttcagat gcacatgtaa atcatttttt tagagcacca
     5761 agaatcctta gaagtgtatt aaatgaatat aaagaaggat taataattgg ttcggcttgt
     5821 gaagctgggg tggtttttca agctgtaaag aaaaacgtgt ctgatgagga aatgaaaaaa
     5881 ataatagatt tatacgatta tattgaagta atgcctatag ataataatag atttatgatt
     5941 gataaaggag aagtaaagga cgaagaagag ttaagggagt tgaataggaa actaattgat
     6001 acagccaaaa aatttgacaa aattccagta gctactgggg atgttcattt tttagacaaa
     6061 catgaagctg tcttaagaaa agttcttaaa tattctcaag gttttaaagt agatgaagaa
     6121 gaaacttatt tacattttag aacaacagat gaaatgttgg aagaatttag ttatcttgga
     6181 gaagaattgg catatgaagt tgtagttgaa aatagtaatc tgattgccga tatggttgaa
     6241 gatataaaac ctataccaga tgatacttat cctccaatta tagagggctc agatgtggag
     6301 ttaagagaaa tgtgttacaa aaaagccaaa cgaatctatg gagacccaat accagaaata
     6361 gctcaaaaaa gactggatag agaattaaat tcaataataa gcaatgggta tgcagttatg
     6421 tatattatag ctcaaaaact ggttgcaaaa tctctttcag atggatattt agttggttca
     6481 agaggctcag ttggctcatc ttttgcagct actatgagtg atataacaga agttaatcct
     6541 cttccagcac attatatttg tgaaaacgaa gactgtaaat attcattctt ttatgaaata
     6601 ggtgaatggg gctcaggtgt tgatttacca gacaaggatt gtccaaagtg tggaagaaaa
     6661 cttgctaaaa acggtcatga tattccattt gaggttttct taggttttga aggtgataaa
     6721 gaaccagata tagacttaaa tttctcagga aattatcagc caacaattca taaatacaca
     6781 gaagaactat ttggagaggg gtatgtatat agggctggga ctataggtac tgtagcagaa
     6841 aaaacagctt ttggatacgc aagaaaatat gtggaagaaa ataatataag tgtaccaaat
     6901 gcagaagtac ttaggctttc aaatggatgt actggagtaa aaagaacttc aggacaacat
     6961 cctggtggag ttatggttat accagactat aaagatgtat acgattttac acctatacag
     7021 tatccagcaa atgatacttc ttgtggtgtt ataactactc actttgatta tcattcaata
     7081 agtggtagaa tacttaaact tgatatactt ggacacgacg gtcctaccat tataaggatg
     7141 cttgaagata tgactggaat aaatataaca gaaattcctt tagatgataa agagactatg
     7201 tctctattta catctacaga agcattaggt gttacaccag aagaaataaa ctgtcctata
     7261 ggttgccttg ctgtaccaga gtttggaact aagtttgtac gacaaatgtt acttgacaca
     7321 aaacctacta catttgcaga acttgtacgt atatctggtc tctctcatgg gacagatgtt
     7381 tgggtaaata atgcacaaga cttagttaga gaagatatag ttggattaaa agatgttata
     7441 tctacacgag atgacataat gaactattta atatttaaag gattacctcc caaaatgagc
     7501 tttacaataa tggagagtgt aagaaaaggt aaaggtttaa aaccagaaca cattgaagaa
     7561 atgaaaaaaa ataatgttcc agaatggtat ataggctcat gtaaaaagat taagtacatg
     7621 ttcccaaaag cacatgctgt tgcttatgta atgacatcat ttaggattgc atattgtaaa
     7681 gttcactatc cagaagcatt ttatgcgact tattttacga caaaagttga ggattttgat
     7741 gcagatttaa ttgttaaagg attagatgct ataaaatcaa aaataaatga aattgagtca
     7801 ctgggaaatg atgcaaccac aaaagaaaaa ggtatgttga ccgttttaga agttgctctt
     7861 gaaatgtatg ctagaggaat aaaattatta ccagttgata tatataaatc agatgcaact
     7921 gagtttatag ttgtaggaga aaaaacatta cttccaccaa tggcagcaat acaaggtctt
     7981 ggaggaaatg cggctataaa catccaaaat gaaagaaaaa atggtgaatt tatatcaaaa
     8041 gaagatttga gaaagagaac aaaaatatca aaaacggtaa tagaaactct tactaatcat
     8101 ggttcacttg agaatatgag tgagagaaat caattgtcat tgttttaagt tagcccaaaa
     8161 agttgcaaat aaaagaattt tatgatatca tagtatagaa aatagtatac tttgcagaaa
     8221 ctaagagtga gacagctgtt ctcactcttt tattttaaat atattattaa aaagctggtt
     8281 tatttttatt aaatatattt tatttaagat tatataaact ggtcatttac aattgaatag
     8341 gaggaacttt aatgaaaaaa aatttagaag ctacaattga ggagattgta actaaaataa
     8401 cggatgaaca tggatttgaa atggttgatg ttgaatatgt aaaagaagca ggagaatact
     8461 acttaagagt atacatagat aaagaagaag gaatatcttt aaatgaatgt gaattagtta
     8521 gtagagaatt aagtccaata ctagatgaaa aagaccctat aaaagaaaat tactttttag
     8581 aggtatcttc tccaggactt gatagagcat taaaaaaaga cagagatttt gttagatatc
     8641 aaggtagaga tgtagattta aaattatata aaccattaaa tggatgtaag caatttgaag
     8701 gtgaacttgt aggattaaca gaagataata atataaaaat cattgcaaat ggtaaggaaa
     8761 tggaatttaa tagaaaagat gttgctattg tcagattagc aataaagttt tagtggtata
     8821 ttaaataggg aggatataaa aaatgaatca tgaatttatg gaagcattag atgaattagt
     8881 agttgacaga ggtatagata aagaaatact tatagatact atagaacaag ctcttttaac
     8941 tgcatacaag aaaaactttg gttcagcaca aaatgttaga gttgaatttg atagagacaa
     9001 gggagatata aaagtatttt ctcaaagagt tgttgttgat gaatcagatt tatatgatac
     9061 tttcttagaa atagagttag cagatgctag agagataaac cctaattatg aattaggtga
     9121 tattatagaa catgaagtta cacctaaaaa ctttggaaga attgcagctc aaacagcaaa
     9181 gcaagtagtt gttcaaagaa taagggaagc tgagagagaa atagtgtata atgaatttat
     9241 agaaaaagaa aatgaaatag ttacaggaga ggttgctaga gcaaataaaa atgttgttta
     9301 tgtaaatctt ggaagaatag aaggtataat gactcaaagt gaacaaatac catttgagaa
     9361 ttataaagca ggtcaaaaaa taaaattcta tatacttgaa gttaaaaaga ctaacaaagg
     9421 acctcaaata gttgtatcaa gaagtcatcc gggtctagta aagagattgt ttgaagaaga
     9481 agttcctgaa atatatgaag gaatagttca aataaaatct gtagcaagag aagcagggtc
     9541 aagaactaaa atggcagtaa gatctataga tgaaaaaata gacccaattg gagcttgtgt
     9601 tggaccaaaa ggacttagag taaaaaatat agtggatgaa cttggcgatg agaaaataga
     9661 tataataaaa tatagtgaag acccagcaga gtttatatca gcagcactta gcccatcaaa
     9721 ggttgttaga gtagatatag atgaagatga aaagtctgcc ttagtaatcg ttccagatta
     9781 tcagttatcg cttgctatag gtaaagaagg tcaaaatgca agactagcag caaaattgac
     9841 taactggaaa atagatatta agagcgaatc tcaagcagaa caagaaatgt taaatcttga
     9901 aaattccaaa gaaatagaag ctgaggatgt tgaagctgaa tttatcgacc aagaagagtt
     9961 agatacttca gaggataatt ttagtgaaga agagtaagga gggatgattt tgaaaaaagt
    10021 aaaaaaagtg cctcaaagaa aatgcatagc atgtcaagat agagactcta aaaaagagct
    10081 tataagaata gttaaaaata aagaaggaaa aatattttta gacccaacag gaagagccaa
    10141 tggaagagga gcatatgtat gtaaaagctc tgaatgtcta aaaaaagcta taaagagtaa
    10201 agcattaaat aaagctttta aaatagatgt tcctgatgaa gtctatgata atctacttat
    10261 ggagttagaa gaatatgaaa aataataaag aaaaagtact ttcgttctta gggctcgcaa
    10321 ctcgtgcagg gaaaatagtg tctggtgatg attcaacttt gcttgacctt aaaaaaggaa
    10381 aagtaaattt gattttaatc gcagaagatg catcaaataa tactaaaaaa ctttttaaag
    10441 ataaatcaac ttttagaaat attccatatc tatttttttc aacaaaagag gaaataggat
    10501 ttgcaatagg aaaatctcca agagcagttg taggaataaa agatgaaaat ttttctaaaa
    10561 agataattga actaatagaa atttaaaata aaataggggg tgatcttgtg tcaaaaacaa
    10621 gagtatacca aatagcagaa gaattaaata tatcaaatga agagcttata aataagttag
    10681 ctgaattaga tataaatgta actgacaaag attcagtatt agaaggtgaa gagttagaat
    10741 tagcacttga aatgttaggt gaagatttat ctcaagaaaa tggaaatgtt atagaaatag
    10801 atggaaagtt gacagtacaa gtattagcaa cgaaattaga taaatctcca tcagaaataa
    10861 ttatgaagct tatgaaaatg ggaactatgg caacaataaa tcaagagata agttttgaga
    10921 ttgctgcttt ggctgccaaa gactatggat ttgaattaac agtagctgaa agtgatgata
    10981 cagaagcttt agaaatagaa gctttaatgg aaattgaaga agataaagaa gaggatttaa
    11041 aacctagacc accagttgtt acagttatgg gacatgttga ccatggtaag acatctttac
    11101 tagatgcaat aagaaaaact gatgtgattt caggtgaagc tggaggaata actcagcata
    11161 taggtgcttc agaagttaaa atcaatggac acaaaatagt atttttagat actccaggac
    11221 atgaggcttt cacatcaatg agagcaagag gagcacaagt tacagatata gcaatattag
    11281 tagttgcagc agatgatggt ataatgcctc aaacagttga agctataaac catgctaaag
    11341 cagcaggtgt acctttaata gttgctatta ataaaataga taaaccaggt gcaaacccag
    11401 ataaggttaa acaggaatta gcagaccaag gattattagt tgaagactgg ggtggagaag
    11461 taatagcagt tccagtatca gctaagaaaa aagaaggaat agatacactt ttagaaatgg
    11521 tattattggt tgctgaaatg gaagagttaa gagctaatcc aaataaaaga gcagtaggaa
    11581 ctgttataga agctgagctt gataaaggaa gagggccagt tgcaacagta cttgttcaag
    11641 gtggtacttt aacagtaggt gacccaatcg ttgcaggtgt tgcatgtggt aaagttagag
    11701 caatgataaa tgcaaaaggt aagagagtaa aaacagcagg accatctaca gcagtcgaga
    11761 tattaggtct atcagaagtt ccacaaggag gagaccaatt tgtcgaagtt ccaactgata
    11821 agattgctag aagtgtagca gcaagaagac aacaaatagt tagagatgaa atgttaaaat
    11881 caactcaaag attatcacta gatgctctat ttagtcaaat gagtgaaggt tctataaaag
    11941 accttaatat agttataaaa gcagatgtac aaggttcagt acaggcagtt aaacaatctt
    12001 tagaaaaatt atcaaatgaa gaagttcaag ttaaagtaat acatggtggt gttggagctg
    12061 taacagaatc agacatatta cttgcagcag cttctaacgc aataataata ggattcaatg
    12121 taagaccagt tccaggtgca gaatctctag gagaaaaaga aaatgttgat ataagaacat
    12181 atactataat atacaaggca atagaagata tacaagcggc tatgactggt atgttagacc
    12241 ctgaatatgt tgatgaagaa acaggaaaag ctgaaataag agaaatatat aaaatatctg
    12301 gtgttggaac agtagctgga tgttatgtga ctaatggtaa gatatttaga aattgtaaag
    12361 taagattagt tagagatagc ataataatac atgaaggtga gcttgcagca cttaagagat
    12421 ttaaagatga tgtaaaagaa gttaattctg gatacgaatg tggtatgagt tttgtaaatt
    12481 ataacgacat aaaagaaggc gatatagtag aagcttatat aactaaagaa gtagaaagaa
    12541 aattataaat tcgtatcaaa gaaaatcagt tagtaaagaa ggtcgatggt atggcatcat
    12601 ataatagaac aagaaggata gcagaagaaa taagaaaagt cgtaagtact atgcttataa
    12661 atggagtaaa agaccctaga ataacatcta tggtgagtgt aactgatgta gaagttacaa
    12721 atgacctaag atatgcttat gtatatgtaa gtattctagg cggagatgaa gaatccacac
    12781 taacaggtct taaaagtgct ggaggatata taagaagaga agtaggaaaa aatataaaac
    12841 ttagatatat tcctgagata gtatttaaac ttgatgattc aatagaaaaa ggaatgtata
    12901 tggatagcct tataaaaaga gttaatgaaa aaaatgctca acaaaatgag gatgaaaatt
    12961 atgatgaata atatggatag tattataaac tatatttatg agggtgacta ttttatagtt
    13021 acctctcata tcagtccaga tggagacaat attggttcta cactttcaat gtattataca
    13081 ttgaagaatt taggaaaaaa tgtatattat gtacttgatg atgaagctcc attaaattta
    13141 aggtttttgg ttgaaggtgt aaaaatctat aagtctaatg aatttaatat gaagaactat
    13201 tctctaattg cacttgattg tggagataag atgagaatat gtgtgagtga tgaaatcaaa
    13261 aataatgcat caaaattaat ctgtattgac catcatgcta gtaatgattc atatggagat
    13321 ttaaattatg tagatattga tgcatcttct acatgtgaat tagtttataa tttattagtt
    13381 agatttcagc aaataaaaga tattaatatt ataaatgaag atatagcaac atgtttatat
    13441 acaggtcttg taactgatac tggaaacttt tcgtattcaa atgtacatgc aagtagcttt
    13501 gaaatggcaa aaaatttact tgttttaggt gcacaaaaaa atactataat acaaaatata
    13561 tatcaaagca attcttctgg ttattacaaa ttattaggag aagctctaaa gggtttggag
    13621 atatttgatt aaaaaatagt tacgcacact cgtgacttta gtcatgagtt agtagctaaa
    13681 atagtcagca tatagggaaa cttatatgta gagataggat aaaacctatc caatactact
    13741 tgaattgctg gaaaccccta aagctaacca aactaaaaca taaggatgaa ataaacctaa
    13801 gtgtgaaagt tacgaaagta gaaaaaattg gttagatggt ataaggttaa accctaagta
    13861 ctttaaaatg ggcaatcagc aggtaa
//
LOCUS       DAGSWI010000108        13820 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.108, whole genome shotgun sequence.
ACCESSION   DAGSWI010000108 DAGSWI010000000
VERSION     DAGSWI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13820
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.108"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            169..1674
                     /locus_tag="MJF80_002857"
     CDS             169..1674
                     /locus_tag="MJF80_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBZ0281506.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            2064..3161
                     /gene="dinB"
                     /locus_tag="MJF80_002858"
     CDS             2064..3161
                     /gene="dinB"
                     /locus_tag="MJF80_002858"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBZ0281507.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            3424..4560
                     /locus_tag="MJF80_002859"
     CDS             3424..4560
                     /locus_tag="MJF80_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281508.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            4553..6100
                     /locus_tag="MJF80_002860"
     CDS             4553..6100
                     /locus_tag="MJF80_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBZ0281509.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            6434..7300
                     /locus_tag="MJF80_002861"
     CDS             6434..7300
                     /locus_tag="MJF80_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBZ0281510.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            7441..8583
                     /locus_tag="MJF80_002862"
     CDS             7441..8583
                     /locus_tag="MJF80_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBZ0281511.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            8967..10205
                     /locus_tag="MJF80_002863"
     CDS             8967..10205
                     /locus_tag="MJF80_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281512.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFITLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            10234..11121
                     /locus_tag="MJF80_002864"
     CDS             10234..11121
                     /locus_tag="MJF80_002864"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBZ0281513.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            11155..11784
                     /locus_tag="MJF80_002865"
     CDS             11155..11784
                     /locus_tag="MJF80_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBZ0281514.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKLKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            11789..12253
                     /locus_tag="MJF80_002866"
     CDS             11789..12253
                     /locus_tag="MJF80_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBZ0281515.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            12503..>13820
                     /locus_tag="MJF80_002867"
     CDS             12503..>13820
                     /locus_tag="MJF80_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBZ0281516.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISF"
BASE COUNT         5220 a         1461 c         2377 g         4762 t
ORIGIN      
        1 taggtatatc ttatttacca taaaactatc ttgaaaaaaa ttaatcttaa ttgtataata
       61 ttactttgag tgaaaaagta ttaacatttt tttataagat gttttactaa aaaaatatat
      121 ataaatatac tgttatcaac aagaaattta atttagtgag gtgttaagat gaaagtagga
      181 tttgaccaca aaaaatattt agaagagcag tctaaatata tcttagaaag agtaaataat
      241 tttgacaaac tttatttaga gtttggagga aaattgatgg gagaccttca tgcaaaaaga
      301 gtacttccag gatttgatga aaatgcaaag ataaaagtat tacaacatat gaaagaaaaa
      361 gttgaagttg taatttgtgt ctatgcagga gatatagaaa gaaacaaaat aagaggagac
      421 tttggtatca cttatgatat ggaagtttta agattaatag atgatttaag aggttatgaa
      481 ctagatgtaa atagtgtagt aattactaga tttgaaggac aacctgctac aactgtattt
      541 ataaataaat tagaacgtag aggaattaaa gtttataagc attatccaac aaagggatat
      601 ccgtcagatg ttgagactat agttagtgat gagggatatg gagcaaatga atatatagaa
      661 actacaaagc caatagtagt agtcacagca ccaggaccta atagtggaaa acttggtact
      721 tgtctaagtc agttgtatca tgaaaataaa agaggtaatg tagttggata ttctaagttt
      781 gagacattcc cagtttggaa tgtacctgta aaacatcctt tgaatatagc ttatgaagca
      841 gctacagttg atttaaaaga tgtgaatatg atagattcat tccaccttga aacgtatggt
      901 gaaatgtcag taaactacaa tagagatttg gaattatttc cagtgcttaa aaaaataata
      961 gaaaagataa ctggaaaaga atcaattttc caatctccaa ctgatatggg tgttaataga
     1021 gttggctttg gaataataga tgatgaagta ataagaaaag cttctataga agaaataata
     1081 agaagatact ttaaaactgc ctgtgagtat aaaaagggac aagttgataa aggtgcttat
     1141 gatagaatga aaatgattat ggaagagctt aacttaaagc cagaagatag aaatgttgta
     1201 ttacctgcta gaaactacag taataaattg aaagaaagtg ccgacaagaa tgatacttgt
     1261 cctgtagttg cattagaatt agaagatggt acaatattaa ctggtaaggg ttctgattta
     1321 atgaatggaa ctgcagcagt agtattaaat gccattaaac atttagccaa tatatctgat
     1381 gatatgcact taatatcacc tgtaatatta gaacctatca taaacttaaa aacaaagact
     1441 ttagctagta gaaatgtttc acttagttgc caagaggtgt taactgcact tagtatttgt
     1501 gctgtaacta atccaactgc acaatttgct atggaaaagt tagcattatt aaaagggtgt
     1561 caagctcatt ctactactat attaaataga gatgatgaac aaacatttag aaaacttggt
     1621 atagatgtaa cttgtgaccc agaatatcct tcacaaaatc tttactatag ttaaaattat
     1681 ataaaaaaat atgggctgtt ttaaagtaat agtttgagca gtccattaca attataataa
     1741 aatcagtagg ctgtcttgta taagacagtc ttttgttttt aaaacatccc ataaattaaa
     1801 tatatatact gttatccata aggaatttta aatttagttc cttggaatac ctattgtaaa
     1861 gcttttatat agtttttgca agtgttttta aggatttttt ataaattata ttgaagttaa
     1921 tggattatag atggttttat ttatggaaaa tatgaatata taaagaaaat aatgaaaaaa
     1981 taccaaaaag aaacataagt ttggtataat taaaaagtaa tataaataaa attaatctat
     2041 ttatacttta aaaggtgata ataatgaaac attatagaaa aataattcat atagatatgg
     2101 acgcttttta tgcatctata gaacaaagag ataataaaaa attaaaagga agacctgtta
     2161 ttgtcggtgg aaatccacaa agccgaggtg tggttgcaac atgttcttat gaagctagaa
     2221 aatttggtat ccattcagct atgccatcag ctgtagctta caataggtgt ccatatgcag
     2281 tttttgtaag acctagattt gatgtttata agagtgtgtc agagcaaata agagatatat
     2341 tttatagata cacggattta gttgaaccat tatctttaga tgaagcttac ttagatgtaa
     2401 caaaaaataa aaaaaatatt gattcatcaa ttgagatagc aaagcaaata aaaaaagata
     2461 tatttagaga agttggtctt acttcttcag ctggtgtgtc atacaataag tttcttgcta
     2521 agattgcatc agacttaaga aagccaaatg ggttaactgt aataaccgaa gaaaatgccc
     2581 aggacttttt agataaatta cctgtgaata aattttttgg tgttggaaaa gttacttcaa
     2641 atactcttaa aaatttaggt ataaaaactg gatatgattt aaggtgttta aatttatttg
     2701 aacttgaaaa tatattcaaa aaaagaggtt atgaattata taaatttgct agaggaatag
     2761 atgatagacc agtggaacca aatagggtta gaaaatctgt tggggctgag actacattaa
     2821 gtcataattt ggatattgat gaagaagaaa cacgcaacat tcttgatgaa ctttgtgaag
     2881 aagtatgtca tagattaaaa aactctgaga agtttggaaa aacattaaca ttaaaaatta
     2941 aatatgagga ctttacaaag ataactagaa gccttagttt agaacactat attgacgaat
     3001 ataatgatat aagaagtggt gtagataatt tgttgagaaa tgttgaggtt aatggaaaac
     3061 aaattaggct attgggagtt acaatatcaa acctaagtga taaaaaagaa acttataaag
     3121 atattacatt atttgaatac atggatagta tccaaatgtg agttttgttt aaatgaaata
     3181 acactagctt taatactata taattgaggc tagtgttatt ttatgaatat ttttatattt
     3241 tttgtttttt gacattatat attatatact atatataaat actttatatt tgtagcaata
     3301 ttcagacaaa tagtgaccta aaaatcgtac tttgtttgca taaataaaat agtatgatat
     3361 aattataaaa acaacttata atattgaaga aggttgcttg ttcgaaaaag gggatgataa
     3421 attatgatag agataagaaa tgtaacaaaa aaaataggaa ataatgttat attagatgat
     3481 atttctctag ttgtagaaac aggtacattg gtagttttga ttggttcaag tggttgtgga
     3541 aagactacta ctttgaagct tataaacaaa ttaataaaac ctacatctgg tgagatatat
     3601 attaatggca aaccaatttc tcaggaaaat gaaatagagc ttagaagaaa aataggatat
     3661 gttatacaga acacaggcct attccctcat cttacaataa aagaaaatat agagctaatt
     3721 ccacgcctaa aaaaggaaaa atccgttgaa gaaattgaaa aaagaacgtt gcaactactt
     3781 gagatggtag gacttgatag tgatgaattt ttgaataagt atccttcaga acttagtggt
     3841 ggacagcaac aaagaatcgg tgttgcaaga gccattgcta ctgatgctga aataatattg
     3901 atggatgagc cttttagtgc tttagaccca ataacgagaa catctttaca agaacaatta
     3961 ttttctcttc aagatgaatt gaaaaaaact ataatatttg taactcatga tatggatgag
     4021 gctttaaaga tagctgataa aatttgtatt atgaaggatg gaagaatagc acaatatgat
     4081 actcccgaaa atatacttag aaagcctgca aatgactttg taaaagattt tatcggagaa
     4141 gatagagttt gggataatcc agaatatata aaagcaagag atattatgat aaaaaatcct
     4201 attgctgtta attctactag aactgtaact caaggaatag aaattatgag aacgagtaag
     4261 gttgatagct tactaattat agatagagca aaaacattaa aaggtattgt tactgtaaaa
     4321 gatatgaaag atattgatga taagtcaata ttacttgctg atattatgtc aagtgagcct
     4381 ttgcatgtaa atgaaggtga taatttagta gaaatactaa atgtaatgaa tcgtaattcg
     4441 gttggatata ttccggttat ttctgatgaa aataaactgg tagggcttat tactagaagt
     4501 agtttacttt ctgttttaag tgaacaattc ttagaaatgg aggtgagtgt tcttggctaa
     4561 tttttttaac tttatactat tacaaaaaga taagattata gagcttttaa tacaacatat
     4621 gagtctaact gttacatcta ttttaatagc tataatagtt ggtgttcctc ttggaataat
     4681 tatttcaaga atctcatctc ttagaaagtt tgtacttgga tttgtaaact tagtccaagc
     4741 agttccgtct atggcacttt tgggtcttct tgtacctata cttggtatag gaagtaaacc
     4801 agccatattt atggtagtgg tttattcatt actaccaatt gtaaaaaata cgtatatagg
     4861 tataacaagt attgacccag tagtattaga atcagctaaa ggtattggtc ttacaagaaa
     4921 tcagacatta tttaaaatac aattcccttt agcacttcct ataataatgg gtggagttag
     4981 aatatctgca gttacagctg ttggtttgat gactcttgca gcatttatag gtgctggtgg
     5041 tttaggatat ttagtatttt caggggttca aactgtaaat aataatatga ttttagcagg
     5101 ggcaatccca tcatgtataa ttgcactgtt agttgattat ttatttagta aaattgaagt
     5161 tgcagttact ccaaaaggat taaatccaaa agcacctaag aagaactata tagcacttaa
     5221 agtaatatct gtagtgcttg ttgtttcaat gttgttcgtt gtttttagtt ctagtttttc
     5281 aagtaaaaaa gatacaataa ctattggttc taaagattat acagaacaac ttatacttgg
     5341 taatgtttat gctgaattag tagaaaaaaa tacgaatctt aaagttaaga agaatttaaa
     5401 tctaggtggt tcatctgttg cgtttaatgc aattaaatct ggtgagttag acatgtatgt
     5461 tgactataca ggtacacttt tggtaaatgt aatgaaacat gctccgatta aagatgctga
     5521 tgaagcatat aatgttgtaa aagatactat ggaaaaagaa aatcagttaa ctttacttga
     5581 ccctcttgga tttaataata cttacacatt agctatgatg cctgaaactg ctgaaaagta
     5641 tggaataaat actatttctg atttgactaa atatggtaaa gaatttacat tctcaccaac
     5701 tttggagttt gaaaatagag aagatggtct tgtaggactt tctagagatt atggtttaaa
     5761 attcaaagat gtaaaagcaa tgaatggaag tttaagatat acagctttag ataataatga
     5821 atctcaggtt atagatgcat ttttaactga tggtcttatt aaaaagttta atcttaagat
     5881 attagaggac gataaaaatt tctttgtacc atattatgca gcaccattag taagagaaga
     5941 tactttgaag aaatatcctg agttagaaaa agttatgaat atgctttcag gaaaaataaa
     6001 tgaagaaact atgagagaac ttaactatca agtggatgaa cttggtaaat caccagaaga
     6061 agttgctcat tcattcttag ttaaagaagg cttagtataa aaaattaata gtttatatca
     6121 aaaatttaag ggagtgtctc aaaatgaact ttttagttca tgaggcactc ttttttatat
     6181 gaaatcaaat atattttatc atttcaacaa taaaaaagaa gattatcttt attttgagac
     6241 agtctcttat ttttatgtat accaaaacca tacctacgat ttttatttat aattaagttt
     6301 caaatagaat gaacaagtac aaccaatttt gaatataaat atagtaaatg tgcttatata
     6361 cgattttatt gtctggttga ttgatattga tagatagctt tctttattga atcgaaggga
     6421 gggggaattt gttatggata actataagaa agtgaagcaa gtattgtgga taattttatt
     6481 tgctaatttt gcagtagcat tattaaaaat cattattggt aatcaaataa aaagctatag
     6541 tatgacagca gacggatttc attctttgtc agatggagca tctaatatag tggggcttat
     6601 aggtattttc tttgcatcta aaccaaaaga taaaaatcat ccttatggac ataaaaagtt
     6661 tgaaattata accagtttat ttataagtgg tatgttgttt gttatagcaa ttaagataat
     6721 tcttagtgca gtgttaagaa tagctaatcc agttgtacct gctataacta tagaaagttt
     6781 aattgcttta attattacac tttttataaa tatttttgta tgcatgtatg aatatagaat
     6841 tgggactaaa ctaaatagtt atgttttgat ttcggattca ttgcatacaa gaagtgatat
     6901 atttgtgtct ctaggtgtat tggttacttt agttggtgtt aagttaggat ttccagtaat
     6961 aatagaatct atagttccta ttattatttc tgcattcata atttattcgg cttatggaat
     7021 atttaggcca tctattggaa ttttggtcga tagagtggct gtagatgagg attatataaa
     7081 agaaattgtg tttgaattta atgaggtaag ggatgttcac aatatcagaa gtagaggtag
     7141 caaaagttct atttatattg acatgcatgt aatggtagac ccatttataa gcgtagagca
     7201 atctcatgat ttaacacata aaattgagaa acaaatacaa gaagaaatta atgaaaatgc
     7261 tcaagtaata gtccatattg aaccttttta tagtttttaa cttagttata aaatacaagt
     7321 tataaaaaaa tataatttat aaaaaaaata ttataccata aataatttat aaaaattatt
     7381 tatggtataa tattttctat aaagaaatta agtcttaata tatctaagga ggaatttaca
     7441 atgagatacg ttgaatatgg aaagacaggg aaaatggttt cagtagttgg ctatggagga
     7501 ttaaggtttg atttagaaaa aagtaatcaa gaaaatgctg atttagtcaa atatgcgtat
     7561 gaaaaaggta ttaattactt tgatacagca ccagggtatt gtgatgacag aagtgaagat
     7621 atatttggac tagcctttag acaaatgata aaggaaggta agaccgattt ttatgtatca
     7681 actaaagaaa agccaaaagc atgtgataca tcagaaaaag ctatagaggc tgtaaaaaaa
     7741 tcaatagaac gattaggcgt atctaaaata aatttttatc atgtttggaa tattagaaag
     7801 atggaacatt acgaactagc aatgagacct ggaggtcaat atgaaggatt attgaaatgt
     7861 aaagaagaag gattgataga ccatatagta ttttcatctc atcaaccagg agatgaggtt
     7921 atagatattc taagtgaaaa taagtttgaa ggtgtaacta tgggaataaa tatattaaac
     7981 tttccatata ggctaaaagg tgccaaatat gctgttgata atggatatgg tgttgtggca
     8041 atgaatccat taagtggtgg aactattcca aagtataata aagaactttc ttttctatca
     8101 aaagatggcg aaagtactat agaatcagct ttaagattca acataggaat ttcacaaata
     8161 agtatatctt tgatagggtt taataaaaaa caagatatag atgatgcatg taaaattgca
     8221 gacgaaaata gaatttattc agatgaggat atacatgata ttgagactag attaaataag
     8281 aatatgaacg aaatatgtac aggttgtggt tattgtaaag tatgtccaaa aggtattaat
     8341 accccagcct atatgctttt ttataatgaa aagcagatgt ttaaaaagag tgatgaagaa
     8401 atgacaaagt tagtttatgg cttaggacat tggaattaca caatgaatag taaggcaaaa
     8461 gctaaagaat gtatatcttg tggaaaatgt gaagttgaat gtactcagca tttacctata
     8521 atagatagat taaaagaaat taaaaaatgg gaagaagatg gagctaatag tgttaaagtc
     8581 taattaattt tatactatat ttgaaatttg tatagtattt agtaatgttt taaaatatag
     8641 ttttaatatt taaggagaca gattataatt ttgtctcctt aaattatgct ttaatttgtt
     8701 atagtttaat tccataagca acattgtata tttttatata aagtttttca aatattttac
     8761 attaatattg cttacaaatt ctcattttat acaacactaa tctatgttat ataaacaata
     8821 cttgactgat tataacatca ataaagtaaa tattgtggtt gtaaataaag agttattcaa
     8881 ttacaataaa acaagtaaga acattatgaa gatggctatt aaatataagg aataaattgt
     8941 aggtgtcagc aaatcctata taatgaatgg atggtgatat attgatgagt aaaatattaa
     9001 aatataaaaa cgagatgttt ttgtttatat tagttgtaat aacttactta ataataacta
     9061 agatattttt ttctaagact actatatttt atgatttaaa caatacttat gatgtattgt
     9121 tagatacaga tacaggagtt ttattcaatt taaatgtatt tgctataagt caagacaata
     9181 gtaagcacat attgttttca gcaattatat caatttttgc atatccaata tacctatttt
     9241 gtacttcaat agcaaatcct gggacaacag attttaatag tgcctatggg tttggactaa
     9301 tttgtctaca aataataact agtgctatgt caataacttt agtctttaat catattaaaa
     9361 agataaaaat gcagagatta actttaattt tacttactat gataatgata ttttcttttc
     9421 cacagttatt tataacttta aatgtagaga gatttatata tagccaattt tcattaatat
     9481 ttttcatagt tattgcaaat aagatgaaag gaaaaaattc ttatttagta gaattagcag
     9541 ctattcctct atttggaata actattagta atatatattt gtattttttt aatatgatat
     9601 ttgagtttaa attaaaaata ggaaaaatgt taaaacattt aattacattt atcctaatgg
     9661 catatatttg tgtagtttca acgaagtctt atgagagttt tatgaacctt ggaaatgtaa
     9721 tacagtatga tacgaaattt ataagtggag aaccaatttt ggaaaagatt gcaatgatta
     9781 tagagagatt attgtattcg gttttttatt ttcctggagc aagcattaaa aaagggttat
     9841 ttttgcaaaa tggagaagtt gctacaatac cagtaattct tacattatta gctttatgtt
     9901 tttgtgtttt atcagttatt gaaaattcag aaaaaagagt tcctaagcta tgtatgggaa
     9961 taattatatt caatctaact ttacatggta tagtaggata taatctagtc aattcaagta
    10021 ttatggctat aaattttagt tttgcagtta taattttatt ggcttacttt acgaaagcgc
    10081 ttagaaaaaa tgaaaagaat atgtataata tatttctatc gctgttattg gttactatag
    10141 taatcagtaa tgtcaatgga tttatagaaa ttttaaacat agggataaaa tcatatcctg
    10201 tttaaaaaat aatattgaaa gggtagtaag tttatgaaac agaccaatac aagtataagg
    10261 cttcttacta aaatttcaga ttatataaag ttaatgagaa taaagcaata tataaagaat
    10321 ttctttgtat tttcagctat aattttttca aacaacatac taaatataaa tttattttta
    10381 aatacgttta ttgcgtttgt gtgtttttgc ctaatgtcat catcagtata tgcactgaat
    10441 gatgctatag acatggataa agataaaaaa catcctaaaa aatgtaatag accagtggca
    10501 agtggaagaa taagcaaaaa aagtgctaat atactatttg tagtattagc tttaatttca
    10561 gtctgtttat caacagttgt tagttttaat ctttctataa ttctcagtct ctatttgata
    10621 aacaatatat tatattctct taaactaaag aatataatac tattagatgt gttttcaata
    10681 tctcttggtt ttatacttag ggtttatgca ggatgtgtag ccataagtgt tagtttatca
    10741 aattggataa ttttatgtac atttttctta tctctgtact tagctcttgg aaaacgtaaa
    10801 aaagaaatag aaactctaag agatgatgca gttgaacata gaaaaatatt ggaagattat
    10861 gacatagaaa atctaaatca aatgatgata gttatattaa gttctacaat tgtatgttat
    10921 gcattgtatt ctacaagtaa tccagaaaag ccacatatga tatttacaac aatatttgta
    10981 gtttatggtg tacttagata taattatata attaacacta caaatgaaaa taatcctact
    11041 gatattgtcc taaaagataa tgctttaaaa ataaacgtaa tcttatggat aattacatgt
    11101 ttaataatat tgatttttta gtgatatttt tagtgatagg atggggaaat acttatgaaa
    11161 aaaagtgtat tattacttga tgtagattat actgttataa atactgatag catgatagat
    11221 ttttttattt actctttaaa aaataaaacc tttaaaacta ttataaagtt gccatacatt
    11281 atttttattc tatttatgta tttaattaga atgatacctt tgaagaaggc aaaagaggca
    11341 atcttttatc caatagtaga ttttagtgaa gaagatttaa aaaagttttt tgatgattgc
    11401 ataatgaaaa aaattaacga atctatgaaa aaggttattt acaaaaataa agaagaagat
    11461 aatgttataa ttatgataac tgcatctcca tatgcatata tgaaatattt taagtattat
    11521 ggtcttgcag acgaggttat aggcacggag tttttttatg aaaattcaag atataaaaat
    11581 caattcattg gagaaaattg taagggaatt gaaaaattaa aaagaataaa ggctgtatta
    11641 ggtaaacttg ggatagagat tgattatgaa aattcatatg catattcaga ctctaagagt
    11701 gatttaccaa tgctttcatt gactaaaaat gcatttttag ttagtaaaaa ggatggtgag
    11761 gttatagagc aaataaattc ataacttaat gtcacttaga ataggaggga atagtttggg
    11821 atatttacac atattaattt tatctatggt tcctgtaaca gaattgaggg gagccattcc
    11881 tataggtatt gcaatgggct taaatcctat atgggtttat gtatttagtg taataggttc
    11941 tacattggta tcgattccct tgatattgac attcagacat gtattacaat ttttaagagg
    12001 gaagaaacta tttaaaggga ttgctgatgt aattgataga aaaataaact caagaatgaa
    12061 gaaattaaaa agtgtttcga taattggtat aatactattt gtaggtatac cactgccaac
    12121 tacaggaaca tggactgcat ctgctatagc ttccatatta aaaatgagaa taaaagatgc
    12181 ttttatggga gtatttcttg gtaatttgtt atcaggggtt atagtttcag ctctttcttt
    12241 acatataata taaaactatc taagtaatat acttggatag tttttttatg agtatgtgtg
    12301 aaaaattttt aataataaat aaaaaatgag ataaagtatt taacttcagt tagttctaaa
    12361 atgtttacta tggcaaaaga aatatagtat agtattactt gtagggaaag tactctaaat
    12421 ttgtctgcta ataaaatgaa atttttatat agaataaaat tttagtgata cattttaatt
    12481 attggattga ggaggtttta aaatggaaaa ttcaaacact aacttaggta gtgaaagtgt
    12541 gggtaaatta ttatttaaac ttgctacacc tgctattatc gcacaaattg taaatgtttt
    12601 atacaacata gtcgatagga tttttattgg tagaatggaa aatggagaag ttgctatggc
    12661 tggtgttgga gtggcatttc caataataat tataataact gcatgtagtt atttaatcgg
    12721 tatgggagga ggtccattag ctgctataaa aatgggtgag caaaacaatg atgaggcaga
    12781 gaaaataatg agtaacagtt tttctgtact ggtgatttta gcaatacttt tgactatagg
    12841 ttttaagatt ggaaaagagc ctttactttg gatgtttgga gcaagtgaat ctacaattgg
    12901 atattcaatg gactatttaa atatttattt aataggtaca gtgtttgttc agatttcaat
    12961 gggtatgaat acttttataa atacccaagg gtttgctaca actggaatga tgacagttgc
    13021 tataggtgct ttaataaaca taatacttga cccgattttt atatttggct ttaatatggg
    13081 tgtaaaagga gctgcattag ctactataat tgcacaaggc gtttctgcta tttgggtact
    13141 tatgtttctc tttggaaaga aaagtatact taaaataaaa aagaagtata tgataccaaa
    13201 agcaagtatc atcttaccag tgcttggttt gggaatctct ccatttataa tgcaatctac
    13261 agaaagttta gtacttatag ccttaaatag taaattacaa atgtatggag gagatttagc
    13321 agtaggttca atggctataa tgagcagtat aatgcaaatt cttatgcttc ctaatatggg
    13381 ggttacacaa ggagcacaac caataataag ttataattat ggttcaggtc aacttgatag
    13441 agtaaaaaag acatttaagt tgtgtttgtt gtcatgtttt acatattcta ctattttatg
    13501 gcttttatta atgatatttc ctgcattttt tgttagtata tttaataaga atccgcagtt
    13561 acttagtatg actagttgga gtataaaaat atattttgct ggtgctttta tgtttgggat
    13621 tcaaatagct tgtcaacaga catttttagc acttggaaaa gcaacaatat ctttggtttt
    13681 agctctactt agaaagattg tacttttaat acctttaata tttatactcc caacattctt
    13741 taatgaaaaa ctttttgcag taattttagc agagccagtt gcagatataa cagctgctac
    13801 tataacagca atttctttct
//
LOCUS       DAGSWI010000109        13757 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.109, whole genome shotgun sequence.
ACCESSION   DAGSWI010000109 DAGSWI010000000
VERSION     DAGSWI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.109"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            353..2452
                     /gene="nrdE"
                     /locus_tag="MJF80_002868"
     CDS             353..2452
                     /gene="nrdE"
                     /locus_tag="MJF80_002868"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBZ0281517.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2465..3436
                     /gene="nrdF"
                     /locus_tag="MJF80_002869"
     CDS             2465..3436
                     /gene="nrdF"
                     /locus_tag="MJF80_002869"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBZ0281518.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3512..4138
                     /locus_tag="MJF80_002870"
     CDS             3512..4138
                     /locus_tag="MJF80_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBZ0281519.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            4533..5162
                     /locus_tag="MJF80_002871"
     CDS             4533..5162
                     /locus_tag="MJF80_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281520.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIITECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            5163..5945
                     /locus_tag="MJF80_002872"
     CDS             5163..5945
                     /locus_tag="MJF80_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281521.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            5938..6816
                     /locus_tag="MJF80_002873"
     CDS             5938..6816
                     /locus_tag="MJF80_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281522.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNKEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIRSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            6849..7808
                     /locus_tag="MJF80_002874"
     CDS             6849..7808
                     /locus_tag="MJF80_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBZ0281523.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKGLIYNAK"
     gene            8122..8814
                     /locus_tag="MJF80_002875"
     CDS             8122..8814
                     /locus_tag="MJF80_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281524.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            8824..9828
                     /locus_tag="MJF80_002876"
     CDS             8824..9828
                     /locus_tag="MJF80_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0281525.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV
                     "
     gene            10076..10927
                     /locus_tag="MJF80_002877"
     CDS             10076..10927
                     /locus_tag="MJF80_002877"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBZ0281526.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            11031..11777
                     /locus_tag="MJF80_002878"
     CDS             11031..11777
                     /locus_tag="MJF80_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281527.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            11777..13693
                     /locus_tag="MJF80_002879"
     CDS             11777..13693
                     /locus_tag="MJF80_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281528.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDTKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     IKPISFEDNECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
BASE COUNT         5406 a         1336 c         2460 g         4555 t
ORIGIN      
        1 caatatatca gaatatattt ttaagctatt tagagataat ttaaattttt aaacaggaca
       61 taataagctt gtattgtttt atatttgagt tgataaaaat ttctttaaat aaaaataatg
      121 tcaattataa agtaataaaa gtttagatat aaaaaaattt gtgtatatac tatatgtagt
      181 atataataga taaagcactg actatatgta gtgcttttgt ttttgaaaaa tacttattgc
      241 atttctataa ttatagatta attttgggat ttcaactgat aagtaccaaa agaaataata
      301 agcaaattaa gtgaaattat ggcacaagat tatgttaaaa ggacggtaaa taatgaattg
      361 gatagagctt aacaatcaag tgataattaa aaatgaacaa ggcaagtacc aattggaaaa
      421 ggataaggaa gccttaagta gttacataga agaatttata cagccaaagt tgagaaaatt
      481 taataaccta gaagaaagat tgaagtattt aatagatgaa ggttattatt caaaagaagt
      541 aataaatcag tatagcatag atgttattaa aaatatatat gatatgattg ataaacataa
      601 gtttgaattt caatcatata tgagtgcaaa taagttttat caaaattatg cattaaagag
      661 caatgatgga aaggaaatat tagaaacata taatgataaa gtgttaattg tagctttgac
      721 actaggaaat ggcgataaag atttagcttt aaacttggca gataaaatag taaaacaaga
      781 atttcaacca gctacaccta cttttttaaa tgctggaaga aaaagggctg gagaaatggt
      841 atcatgtttt ttactatctg tagaagatag tacagaaggt atatcgtatg ctatatcttc
      901 atcaaatcac ttatcaaaga ttggtggagg agttgcacta aatttatcta aacttagagc
      961 atctggcgaa tctatcaaag atatagaagg tgcagctgga ggtgttgttg gagtagctaa
     1021 aatgttagaa caatcattta gttactttaa tcaaatggga gctagacaag gttctggtgc
     1081 ggtgtacctt actgtattcc atcctgattt tgaattgttg atggatacta aaaagataaa
     1141 tgcagatgaa aaaataagac ttgctacatt atcattagga gctataatac cagataaatt
     1201 tatggagttg gcagaaaaaa atgaagtagc atatgcattt tatcctcata ctgtgtataa
     1261 gaaatatgga gtttctcttg atgagataga aatggatgag tggtatgata aattggttaa
     1321 tgatagtgat ataagaaaaa aagaaataaa tcctaggcag atgttgacaa aaatagcaca
     1381 gatgcaacaa gaaagtggat atccatatgt agtgtacata gatacagcta atagagaaca
     1441 tacattaaaa gatgttggaa ttattaaaat gtctaactta tgttgtgaga tatttcaata
     1501 tcaaacacct tcagaaatag aggggtatgg aggaaaaaat gaatggggac aagatataag
     1561 ttgtaaccta ggttctttaa acatagctaa tgtaatggat aataaaacta tagaaagtac
     1621 agttgaaaca gcaataagag cactgtcatt tgtagcagat agcacagata taaaaccagt
     1681 gccaacggta agcaatagta acagcaagtc tcattctata ggattaggag ctatgaattt
     1741 acatggatac ttagtaagag aaaatatttt atatacttca gatgatgcta tagatttttc
     1801 taatgtattt tttgcaatgg ttagatacta ttcaattaaa gcttctatga aaatagctat
     1861 agaaagaaat caaacttttg aaggatttga taaatcagaa tatgttaagg gaagaaatag
     1921 caaggtttta tcaaaatact atgaacaatc ttatctacct aagtcagaaa aggtaagagc
     1981 attatttgaa gggatttata taccaacaaa agaagactgg acaaaattat tggatgaggt
     2041 aaaagaaaaa gggatatata atgcatattt aatggcagta gcaccaactc aaagcattag
     2101 ttatgttcaa aatgctacat caagtataat gccaattact gagccagtag aagtaagaac
     2161 atatggagac tctactacaa tatatccaat gccattttta acaaatgata atatgttgta
     2221 ttatcaatca gcatacagga tggatatgag aaaggtcata gatttagtgg caacagttca
     2281 aaaccatgtt gaccaaggta tatcaacaac actgtttgtt acagatgaaa agacaactag
     2341 agatatagct agacattaca tatatgccta taaaaaaggt ttgaaaagtt tatattacac
     2401 aagaacaaag atgacaagag atactcatga gtgtctagtg tgttcagtat agggagagaa
     2461 atatatgaca tttaatttac ataaaataca taatgcagtt aactggaata gagaagaaga
     2521 tggatttaca caagcttttt gggaacaaaa tgtaaaacaa ttttggcttc ctgaagaaat
     2581 atctgtttct aaagacataa aggtatggaa tgaactaagt aataaagaaa aggaattata
     2641 taaaaaagta ttgggtggtt taacattact ggatacaaaa caggggaata atggaatacc
     2701 atctatgatg agtttaacag aaaacctaca aagaaaagct gtcttgtctt ttatggggac
     2761 tatggaagaa atacatgcaa aaagttattc ttctatattt atgactctac tttctaattt
     2821 agaaatagat gaattatttg aatggataga aacagaacca acattacaaa gaaaggctga
     2881 tttagtttta gctcaatacg aaaatactac aaatcaagag ggattatacc tatcaatggt
     2941 tacaagtgta tttttagaga gtttcttgtt ttattcagga ttcttttatc cattgtatct
     3001 atctggtcaa ggaaagatgg tagctagtgg agaaataata tcgcttattc ttagagatga
     3061 gtctttacat ggtaagtata taggattgct agcacaagaa atatacgatt catttgataa
     3121 aatggataag gagatgttag aagagaagat gtattctata ctttatagcc ttatggagaa
     3181 tgagatagag tatacaaatg ttatatacag agaaagtggg cttgaaaaag aggttgtgaa
     3241 ctttttaaaa tacaatgcaa atagagcttt agaaaatcta gggtttgaga agctatatac
     3301 tgtagatgct ataaatccaa ttgtattaaa tggtcttagc actgaaacca aaacacatga
     3361 ttttttttca actaagggta atggatacca aaaaggagtg tatgaggaat tagaggatga
     3421 agattttata atataattta taattatgac acactgttgt cacttttagc gtgataatat
     3481 caatgtataa aatatatatg gaggtatttt tatgaaatat gaatggagaa aaagagataa
     3541 agagttatat ttacctaaaa agaatcctgt aatagttgaa ataccagaac agaaatttat
     3601 catgttaaaa gggagtggag acccaaattc taaagaattt actgaagcaa taggagtact
     3661 ttattctttg gcatatgcaa taaaaaccat gcctaaaaaa ggaattgtac cagaagggta
     3721 ttttgactat acggtttttc ctttagaagg tatatgggat aagggagaaa aaagtaaagg
     3781 cttagatgta cttattaaag aagatttaat ttacactata atgatacgtc agccagattt
     3841 tgttacacaa gacctgttgg ataaagctac tgatatagta aagaagaaaa aacctcataa
     3901 tcttcttgaa aaagttagat ttgggtctat taatgaaggg ctatgtgttc aaatgcttca
     3961 tataggttca tatgataatg aaccagaaag ctttgacatt atgaacgaat tttgcaaaaa
     4021 aaataaactt ataagaaaat cttacacaca tcgtgaaatt tatataagtg atgctagaaa
     4081 agtatcgcct gaatcattga agacggtttt acgttttgaa gtagaaaggg aagtataaat
     4141 attttgaata atttataaga gttataaaaa gtgaagtctg ttgagacttc attttttgat
     4201 gcaattttaa aattaaattt gatgtttcat aatattttaa aaactttcta cttttattag
     4261 tgtgaataac tgttgctatt aaataataat actaattata taagtattat tatataatat
     4321 ttatagatta ttatataaat agattattag taagatgtat gcattattaa ttaaaataaa
     4381 ttacatatac tatttaatat ttcatttaaa atattaaaaa atgtagataa aaattgtttt
     4441 attaagaaaa aattaatata ttgacaaaat ttaagtgctc atttaaacta ttaattgata
     4501 ataatattca atattaaata ataggaggcg ttatgaataa atatcttcta ataaatccag
     4561 tggcagaaag aatgtatgga gaaaagtttc accttattaa agcaaatttg ataaaaaagg
     4621 ggtacataat aacagaatgt gaaccacagt tagaatatgt aaaaaagcag tacaaagagt
     4681 atacaaaaaa gactgaaaat acaatgttgg attgtagatg tccagaaagt ataagtcttt
     4741 taaaaagaaa taatttgata aataattttg aagtacctat gatagaacca atattagtaa
     4801 ggacttgtag ggttttatac gataaatata taaaaagtaa agatgatatg ttggtagtaa
     4861 cctgtccatg tacacaatta agagactttg caagtgaaaa gtttaaggat aagggtaatg
     4921 ctatgtttta tacatggaaa gagtttgctg aacaagaagg tgtaaaatca cttggaaaaa
     4981 tagatgaatc accaattcca ttaggatatt tcaaagatac ttttgaaaaa gttcttgaag
     5041 tatcaagtga agacgaaata ttaagtgaaa tacaaaaaat aagaaatggt tttgaagata
     5101 agtatgacat agtagaaatg ttttattgta aagatggttg taataatgga gatggtttgt
     5161 aaatggtcaa taaatttaat aataagtttt taaggctagt aaagagtgtt ctgatatttt
     5221 ttataattct tttattatgg aaaattacaa attatcttgg tatttggagt gattatatat
     5281 taccatcacc tgaaaaggta tatagtacat ttttaaatat gataagtgat ggttcaatat
     5341 ttattaatgt atatgcaagt atgaaaagag ttttgatagg ttttgcaata agtactgcta
     5401 taggtgtgcc tttagggata ttctttggaa tttatagtgg agtatacgaa tactttaaat
     5461 ctctaataaa ttttttaagg aatacacctc cccttgcttt aataccaatg cttatattat
     5521 ggtttggtat aggagaggaa tcaaaaatta taattatagt tttagcatct tttttcccaa
     5581 tctttacaag cacattgaaa gggattaaaa attgtgactc aaaactgatt gaagtgggta
     5641 gagtatttga gttttcaaag cttcagataa tatttaaaat aataatacca aatgccattc
     5701 ttgatattgc tgttgggtta aagttagctc ttggatatag ctttagagca attattgggg
     5761 cagaacttgt agcagcttca tctggtcttg gatacttgat atctgatgga aaagaaatgt
     5821 ctagaacaga tgttgttatt gtagggataa tcgtaattgg acttttagga ataattacag
     5881 attacatatt ttcgattatt gtgaagaagg taagtaaggg aaagatggtg gaagcatatg
     5941 aataatttag tatgtgactc tggatatata attagaaatt tatataaaag ttataaagtt
     6001 gacaataaag aacatgtagt tcttagtgat atctctttaa acatagatag aaaccatata
     6061 acagtaattt taggtgaaag tggttgtgga aagactacct tgcttagagc aattgcatca
     6121 cttgaaggta taaatagtgg tgaaattagg tttattaatg ataataaaga gtatagacct
     6181 aatgttggtt ttgtttttca ggagagtaga ttaatgcctt ggttaaacgt aagtgaaaat
     6241 atagcttttc ataatcaaag aaaaaatact attttaaaca aggtgcttag aaaatttaaa
     6301 cacaataaaa gaattaggag taaaattata aatgataatt ttagaagctt agaaacaaat
     6361 gataatttga acaaatcaaa taaatataat aggaaaagta atattgatgt agaaaaatat
     6421 cttaagatga tgaacttgca gaaatttaaa aactcttatc caaatgaatt gtcaggaggt
     6481 atggcgcaga gagtaagcat agctagagct ctttctttca atccagatat gttgcttatg
     6541 gacgagcctt tttcggcatt agattatttt acaaggattg atatgcaaaa tgaagttata
     6601 agaatacata agtcaactaa taaaggcgtg atatttgtaa ctcatgatat tgatgaagcg
     6661 ttaaagatag gcaagaaaat agttgttttc acagacgaaa gaaaagtaaa agaatttaat
     6721 atagaagatg gttataatag agatttaact tcagattact atattaaatt aaaaaaagat
     6781 attttaatga caatgaagtc aaataaaggc atttaaagaa attaataata ttttaaggag
     6841 taaagtaaat gaaattaaaa aagctattga taggtataac gatattaaca cttgcaacag
     6901 catctttagc tggatgtgct aaaaaaaata atggagaaaa actttcagaa ataaatttaa
     6961 cttatgtaaa atcaccatta aatgttcctt ctataattca gaaacaggat gatttatttg
     7021 gaaaggaatt taaaaaggat aatataaaag ttaacttcca cgaaattact acaggtccag
     7081 agcaaacaca agctttagct gctggagaaa tagacttctt acatgcacta ggtggtacat
     7141 cagctttaat agctgcatct aatggagtag agttaaagat attaaacact tatagcaggt
     7201 caccaaaagg atttatgatt ttgactaata ataattcaat aaaatcagca gcagatttag
     7261 taggtaagaa ggtggcaggc ccaaaaggga ctatattaca tcaggtattg atttcagcac
     7321 ttgataaaga aggattaagt atggatgatg tggaatttgt taatatgggt attccagagg
     7381 catcagcagc tctttctgat ggcagcgtag atgctgcatt gattgctggt ccagcagctc
     7441 ttaaagctat gaagtcaggt agtaaacttg tagcaaatgg tgaaggttta gtagatggta
     7501 taatagtaac tgctgttagc acagactttg cagagaaaca tccagaaata gttgaaagat
     7561 ttatgaaggt tgaaaaggaa actttagaat atgtaaataa taattttgat gaagctatgg
     7621 aaaaagtagc taaagaagtt gatttaagtc ttgaagaaac taaagaatta tatgcttggt
     7681 atgattttag tttagatata acagataaag acatatcttc gcttgaagat acacaagact
     7741 tcttaataaa aaataagtta caagagaaaa aagtgaacat aaaaggatta atctataatg
     7801 ctaagtagaa actatgataa aataatacta agtaagaaat ataataaaag tttaagttac
     7861 cttattaagg taactttttc tattttgaat tagtaatttc aatttatttt agaatgatta
     7921 gttgataata atgattaatt aatcataaaa agatactttt ttatagagtt ttattaagat
     7981 gtataataat atggttatac ttatgaagtc agtaattgtg aagttagtaa tatgattaaa
     8041 tttatatatt gtaaaaagat tataatatat taaaattgga ctgttatatg atgagtaaat
     8101 aggtatatta ggaggaatta tatgagttat aaagtatata ttgtggaaga tgatatatca
     8161 ataagtacat tacttaaaga ttatattgag aaatatggat ttgaagtgat tgttgaagag
     8221 aactttgatg atataatgat tacatttgaa agttttaagc cacacttagt tctattagat
     8281 gtaaatttac ctaaatttga tggtttctac tggtgtagat gtattaggca acagtcgaat
     8341 ttacctataa tttttatatc agctagagat ggtaatgtag accaaattag agctttagaa
     8401 agtggagcag atgattatat aacaaaacct ttttactatg atgtagtaat ggcaaaaata
     8461 aagagtcata taagaagggt atatggtgaa tattcaccta aaatagacga aagaatagtg
     8521 gagctagatg gattaaagtt ttatccagaa aggttagaac tagaattaaa tggtgagaat
     8581 ctaattataa ctaaaaaaga aggtatactg attgaatgtc ttatcaagag atacccaaaa
     8641 gtagtaagta gagattatct tttagaaaag atatgggatg atattgagtt tgtagaggaa
     8701 aatactttaa atgtaaatgt tagcaggata aggaaaagat ttgaggaatt aggtatagat
     8761 aatgctgttc agactgtccg agggttagga tataaattaa ataagaattg gtaaggagaa
     8821 agtatgaagt tatttttaaa agattataaa ggttatattt ttatatatta tataggcata
     8881 gttatgacaa ttatttactg taatcttatg aagtttatta gttttggaga gagtttatat
     8941 attcttttat ttagttcttt tatattgtta tgttttttaa tatataagta ttataaaaat
     9001 agagaagtat ataatctgtt tgaaaaagga gtacattctt tggaggagtc atttttgtat
     9061 ttaggaagct cattcttggg gagaaatata tcagttattt taaaacaaca acataaattg
     9121 tatcaatcaa tgatacaaga gcataaaaag atacatagtg aacatttaac tttcatcaac
     9181 caatggattc atcagatgaa aacaccaata tcagttataa gccttcagct tcaagattat
     9241 gagggagaag aagtaactga aaatataaga aaagaaatag atagattaaa taaagggtta
     9301 gatatggcta tgcattttgc aagggcagat aattttcaga aagactttat agttgaaaaa
     9361 gccaaattaa atgaaattgt atctagtatt gtaaatgaag aaaaaaggtt atttataaaa
     9421 agtggaatga ttcctaagat tcaagtagac aaatttactt atgtttatac agatgtaaaa
     9481 tggatgaaat ttataatagg tcaattaatt acaaatggag tgaaatattc taaagattat
     9541 agtaactacc taactataaa atctgaagaa aataataaat atgtaatttt aagtataata
     9601 gatgaaggaa ttggaattgt ttctaaagat ttaaaaaggg tatttgaccc attttttaca
     9661 ggagaaaatg gtcgtaagtt tggagaatct acaggtatgg gtctttatct agtaaaaaag
     9721 gtatgtgatg atttaggtca taaagtaagt ataaaatcag aagtaggaaa aggtacagaa
     9781 gtttcaatct actttaataa agatgtaaat agtttaataa aggtgtaaat tatctgttac
     9841 aatagggttt ttagttaatt gtactttttt attatacaat ttattaaatg aaatattcat
     9901 agttaaataa tcaaaattta agagagttta tttgcaaacc ttacatagat gtaaggtttg
     9961 tgtaatgttt gcagatagtt aaattttcta atctaaatta caattaatat aagaaataac
    10021 ataagagaaa taacataaaa aaagaaattt tgtaaaaaag gaaggatgtt tattgatgag
    10081 tttagatgtt atttttatat tgaaatcagt cataatagct atagtagagg ggcttacaga
    10141 atttattcca gtttcatcta caggtcatat gattttggtt ggtaatttaa tagattttaa
    10201 ggggcaattt gctgaaatgt ttgaggttgt aatacaactt ggggctatat tagcagtagt
    10261 agtactttat tggaaaaaga taaaagatag cgttatagaa ttttttaaat ttatatttac
    10321 tggtggaaaa gaaggaaaga ttggttttag atttggaatg aatgtaatta taggttgtat
    10381 accatttgcg attataggag ttctatttta cgataatata aaatcattgt ttaatttaca
    10441 atctgtaatt ataggattta tagtgggtgg gattttatta ttagtagtag aaactttgtt
    10501 tagaaaaaag aatcattcca cagataatat agataaaatt actccaatac aagctttaaa
    10561 ggttgggaca ttacaagttt tgtctgcatg gccaggtatg tcaaggagtg catctactat
    10621 tatgggtgga tggatagcag gacttaattc tcctacagca gctgagtttt ctttcttttt
    10681 agcagtacca gctatggtgg cttcttcagg aaaagattta tttgaatttg attattcaat
    10741 aatgacacca acattgtgga ttgctttggt agttgggttt atagtagctt ttattgtatc
    10801 aataatagtt atggaaaaat tcgtgaattt tttaaagaaa aaaccaatga gagtgtttgc
    10861 tgtatacaga ataatcatgg gtgtagtact tgcagtatta gcttttacta acataattag
    10921 tgtatagaaa aaatagattt attatctata aaaattaaac taagataaat tataattaac
    10981 aattatatat atgttatttc aaaaattaaa ttaggaagga tagttaaata atgaaaattt
    11041 taactgtaaa taatttaagt aaagtatatg gtaaaaaaat aattttcaac gcattaaatg
    11101 acataaattt tagtatagaa gatggagaat ttgtaggaat tatgggtcca tctggtagtg
    11161 gaaaaactac tcttttaaat atgatttcta ctattgacaa gccaaccaca ggaactatgg
    11221 aattaaaggg caagaatcct ttattgttaa gaggtgagga acttgcatta tttagaagaa
    11281 gagagttagg ttttgtattt caggatttta atttattgga tacattaact ataggagaaa
    11341 atatagtatt gccattgaca ttagataagg tcagtgtaaa agagcaagat gagagattga
    11401 atgaagtatc tacaatatta ggtataaaag atttattagg aaaaagaact tttgaagtat
    11461 caggtggtca agctcagagg actgctattg ctagagcatt gattaacaat ccatcaatac
    11521 ttttagcaga tgaaccaaca ggtaatttag attcaaagtc atcaaaggtt gtaatggagc
    11581 tatttcaaaa gataaataaa gaaaataaag ttactactat gatggtaact catgacccat
    11641 tagcagcgag ctactgtagt agaatcttat tcataaaaga tgggtctata tataatgaaa
    11701 tatataaggg tagtagtaga gagcagtttt atcaagagat aatggatgta ctcacattat
    11761 tgggaggcga taactaatga atataaaaca gtttgctatt aacaatattt caagaaatat
    11821 taaagcgtat ttaggatact tagcaagtat tgttatatca tcttcactgc tattttcttt
    11881 tattatgttt acaagtcatc cagatttaga tgtatctcaa tttcctgatt atttaaaaga
    11941 aggtctcaag atgagtaaga taattgcata tctattctta ttcttctttg tgttttattc
    12001 agttagtgtg tttcttaaaa gtagatataa agaatttgga atactttata ttacaggtat
    12061 atcaaagcgc caagtaatga aattaatctt tatagaaaat atgataataa atatagtatc
    12121 ttctgtaatt ggaataataa taggcttaat ctttgcaaag atatttttag tggttatgtc
    12181 tactttttta gaattatcgc cattaaaatt ctatatacca tttaatgcga tattgtctac
    12241 tttaatctat tttatgattt tagctgtatt aacatcaata tttacttctt ttatagttaa
    12301 agaaaatcag gtgttgaagt tacttaaagg tacaaaaact ccaaaaccag agcctaaaac
    12361 atcactaatg cttgctatat taagtatttg tttgtttata atagcgtatt ataatgctat
    12421 tacctcaacc gaccaatata caacatatct gcgcttagtt ccagttacat cccttactat
    12481 agtagcaact tacttgttct tttctcaatt tagtgtattt tttatgaaga agctaaaatt
    12541 aaacaagaga ttttatagaa aaaatacaaa tatgctttgg atttcgaact tgatttataa
    12601 ggtaaaggat aatgctagaa tatttttctt gataactata acttctgcag tggcatttac
    12661 tgctataggt acagtttatt ccttttggaa agatgtagag cgtcaaatta atttaatata
    12721 tcctaacaca atatattatt caacaatgac attgcataat gacactaaaa agcctgatag
    12781 caaagaaaaa gacaaggaaa gaatgatgtt tatagaaaat aatctaaaaa aagagaatgt
    12841 agactataca aggattaatg gtgaatttaa aactgtattt cccaagaaaa gtgattttaa
    12901 tgtcagaatt atgaaagaat ctaagtactt agaaattaca gaaactatag ggattaagcc
    12961 tataagtttt gaagataatg aatgtatatc attattatca tcaagtttac caggaaataa
    13021 gaacgtcaaa gaaaatatta ttgtagggaa tagaggttta aaggtggcaa aacaagtgga
    13081 agaatgtgtt atgccagcat attacaaaaa tatgtatgtg gtaaaggata atttttatga
    13141 tagtatagaa tcaaaattta tagtggatag atttactgca tttgaagtag aggattcaag
    13201 taaaactata gagatatgca gagaatttga aaataaattt aataatgagt cagggatgca
    13261 accatatttg tttttctcta aagaattaat gataggaact gggaaattaa tgtattctac
    13321 atttatattt ttagcaatat ttataggttt aattttcttt gttaccacaa gtagtttttt
    13381 atataataaa ttttatatgg attgccaagt tgataagaaa aagtatgaac agttaaataa
    13441 attgggtatg acatataatg agattaaaaa agcttcaaca attgagattg gaatagtatt
    13501 tttgtttcct tatgtagtgg ctgttataca ttcaatattt gcacttactg cacttaaaaa
    13561 ttcttttgat atagaagtag catcatcagc tgttttggta atgggaagct tgttcttggt
    13621 tcaaatagtt tattttttaa ttgtaagaaa taattattta aaagagataa ggttaaattt
    13681 agtcaatttt tagttactat tattaaatga taataaaaag ggtttaaaat taattaatat
    13741 tactggaatt atagatg
//
LOCUS       DAGSWI010000110        13512 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.110, whole genome shotgun sequence.
ACCESSION   DAGSWI010000110 DAGSWI010000000
VERSION     DAGSWI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13512
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.110"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(332..1213)
                     /locus_tag="MJF80_002880"
     CDS             complement(332..1213)
                     /locus_tag="MJF80_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBZ0281529.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEADQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNEND"
     gene            1669..2643
                     /locus_tag="MJF80_002881"
     CDS             1669..2643
                     /locus_tag="MJF80_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281530.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(2759..3820)
                     /locus_tag="MJF80_002882"
     CDS             complement(2759..3820)
                     /locus_tag="MJF80_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBZ0281531.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(4431..5282)
                     /locus_tag="MJF80_002883"
     CDS             complement(4431..5282)
                     /locus_tag="MJF80_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBZ0281532.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(5299..6219)
                     /locus_tag="MJF80_002884"
     CDS             complement(5299..6219)
                     /locus_tag="MJF80_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBZ0281533.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(6329..7243)
                     /gene="murQ"
                     /locus_tag="MJF80_002885"
     CDS             complement(6329..7243)
                     /gene="murQ"
                     /locus_tag="MJF80_002885"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBZ0281534.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(7342..8439)
                     /locus_tag="MJF80_002886"
     CDS             complement(7342..8439)
                     /locus_tag="MJF80_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBZ0281535.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(8672..10018)
                     /locus_tag="MJF80_002887"
     CDS             complement(8672..10018)
                     /locus_tag="MJF80_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBZ0281536.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(10107..10415)
                     /locus_tag="MJF80_002888"
     CDS             complement(10107..10415)
                     /locus_tag="MJF80_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0281537.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(10676..11074)
                     /locus_tag="MJF80_002889"
     CDS             complement(10676..11074)
                     /locus_tag="MJF80_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281538.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(11076..12236)
                     /locus_tag="MJF80_002890"
     CDS             complement(11076..12236)
                     /locus_tag="MJF80_002890"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBZ0281539.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILSKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(12667..>13512)
                     /locus_tag="MJF80_002891"
     CDS             complement(12667..>13512)
                     /locus_tag="MJF80_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBZ0281540.1"
                     /translation="YINFRNSYTNLYNCIENSGEIDSYLVNGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSLDS"
BASE COUNT         4342 a         2369 c         1474 g         5327 t
ORIGIN      
        1 atctattcct atttatataa ttgtttctta ttataattaa tatacttttt aatcaaattt
       61 aaacagaata attataccaa tatataaaaa aatttctaaa aaacaataaa attaagttat
      121 aaacaccaat atatttctat tatttcaata agtatacaat tagattagaa tctaacgcta
      181 gtaataaaaa attactttcc caaaatagat ataagcctca ttttatcgtt caaataatga
      241 aaatatattt gatttcatgc aaaaagagtg cctaatgaac taaaaagttc attttgagac
      301 actcattttt ttatataaat ttatatagtt tttaatcgtt ctcattcaac cactcagtta
      361 agacttttat atatttctca ttttcttcaa cgaatggcat atgtcttgaa tactcaaata
      421 attcccactt actattaggt attctatcat acatagtctt tgcaatatat ggtgaacata
      481 aatctattgc tccactagtt actagacaag gctcttttat ttcatgtaat ctatctgtaa
      541 attcatatcc agaaagagtt cctgtaggag aaaattcatt ttgtccccat cctacaatat
      601 atgcttcaga acctgatttt ttaggtcttc tcaggcactc tggagaatct tctgtcactt
      661 cacctgcaca atacatttcc ataaatcttt ctaatgcatc attatattct ttgctagagt
      721 aatctccagt atttactgca tcaagtaatg ctttttggtc agcctcactc atatatgata
      781 ttcttctttt ttgttctttc tcccaaagtt ttgcagaaga aagagtagat gaaaggatgt
      841 aagacttaat accttttggt ttatattcaa tagcatacca aatagcctgc atacctcccc
      901 aagattgacc taacagatga atttcatcaa gtcctagatg ttttcttagt tcaataagct
      961 cctctatcca agtatctgca ttaaatagct caggatgccc ttctacaaat gaatttccac
     1021 aaccaatttg gtcatacatt attacttgtc taccactttc agctatctta tccaatacct
     1081 caaaatagtt gtgtgtagaa cctggacctc catgaagtaa tactaatggc ttcttccctt
     1141 ccgtattttc tcctactata cggtaatacg ttttaaaacc tttaaaaggc atatatcctt
     1201 cagtaatttt catgtttatc tcctccaatt tgtaattata aacaataatc aagttcatta
     1261 acaatactat ttaaaacctc ttatacatat ttgattatgt attttaatct ttatacctac
     1321 ttttataaat agtataacat aaaagttaaa aatctctata ctaaaccaat tataaaatta
     1381 tattaactat ataattttat tttttaataa tagtatatac atatttgatt attttcattt
     1441 gctgcttaaa atcaatttta agttttcttc ctataataaa cttcctcttt gtggtctcat
     1501 gttagtctct aaatttaaaa ttttacaaaa aattttcata aaataaaatt atattgattg
     1561 tatctaaaca aatagggcaa taaaataatt aagtatagtt acttaccttt caagcaatat
     1621 tagttttatg ttagaatttt attatattga tttacaggag gtttgcattt gaataatatt
     1681 aattcactta aagtaataaa attatctctt ggatgttctc ttgctatatt tattgcttgg
     1741 ctattaaaat tagaatattc aatggttgct ggagttatag tactacttac agtaaaggac
     1801 actaaaagag aaactcttaa aggctccatt ggaaagattt atggttttct tttatgtact
     1861 attttttcat atttatgttt taatatttta ggatataatt tgacttcatt ttcaatattt
     1921 atatttataa ttataccact ttgttttttg ttaaatattc aagatgttat tgctatgtgt
     1981 gtagtcatag catcccatta ctaccttcaa ggagaaactt ctatcaaatg gattttaaat
     2041 gaagctggaa tatttgcagt aggaactgga ataggtgtct taataaatat gtatattcca
     2101 acaaatatac ataaaatcca tgaagttcaa aaaaagttac aggaagaagt gagtattgta
     2161 cttatagaca ttgcagatat tattgtaaat cctaaaaaag aaaatggata tagtagagat
     2221 ttatatacat taaattcact tattgacagc agtatttctg aaacttacga taatattaat
     2281 aatactttat taagtgatac tagattcttc ttagaacata tggatattat aaaaagccaa
     2341 cgggatatct tagaaaattt gtatagttat gtatctcaac taaattctac accaccacaa
     2401 gctcatattc tatctgcttt tatacataag ataggttata ctgaatttga agctgaaaca
     2461 ggcaatcttc ttcttgaaga attaaagaga ctaatgctta gtatgaaaaa ccagcctctg
     2521 cctgttgaca gaacagagtt tgaaaataga gctattttat tcctatgctt gactgagtta
     2581 aaacagtttt tagttaatcg taaacatgct cagatgctta gagataataa tttttataaa
     2641 taaaaagggg agaaatctag cagttgacct gactacaaaa tatcagatat aatattaaat
     2701 gcgcccctag taagagggcg caatgtttat aacttattct actgtcatga ttaaatcatt
     2761 attcatatcg aacatacatt atttgaccaa gtccaatatt agctttttca tcagtgattt
     2821 ctattttaat agtcacatca ttttttttac agtacttcgc taattctgca ccagaacgat
     2881 ttttaccact aaaatctaaa gaaaatggct tatcatattt aaaaggatat tcaacaggtt
     2941 taccctctgg tgctaatgca aacatcccat atggtgtgtt cataaatcct attacagcaa
     3001 tcgtaagatt tgatgggtct aaatttgatt cactacctgt aaatgtaatt gtatttactg
     3061 atgaattaac tacactacat cgaacacctt catacattac atgtttttca tttatatctt
     3121 ctcctttttt tccaaaattt ccaacagcct ttgccccttc ttgatgtggt aacatagtgt
     3181 gcttcccagg tggtgctggt ttctgtgtct gttctggttt taccgtctgt actattgcgc
     3241 caacttttgg atatgctgtc ttatcaaatg gtaatgggtg gctaagactc ttgtatatga
     3301 tatcctttcc tgctgattta ttagcatcaa tatctacatg atacaaacgc ccatcaccaa
     3361 aacggataag attccactgg tggtcatttg atgtgctgtg tacataataa gattcaattc
     3421 ccatagcatc acacaaagct ttcatgctcc tagccattcc tgagcatgca ccttttttag
     3481 taaccaatgt ataggcagta tttgctccct ctactgcatt ccagtcaaca tatgttgtgt
     3541 tatttttaat gtgattgtag gcaactaaaa tcttagcatc tgtggtcata ttctttgtga
     3601 ttttcttgtt cataaaatct gctgttgctt gtgctactga gtttagttgg gcttgtgaca
     3661 gctttggacc atatacgcta ttggttccag tgacatagct ttttggtgtt tttgctgtta
     3721 cctctttggc tgtcatacca taaactgtca tactcatacc tgagattaaa actattgttg
     3781 taatcaataa cttggtagct aacttcctaa ttttcttcat acttaatatt ctcctttata
     3841 aaacattgtt acttcctatt ctctaccatt aataaaatta atttaggatt taaaaaggtg
     3901 gattgtcaac atgtatattt ctccattcat acattcgtga aatatttaaa tttcatggaa
     3961 aatctaatag atattttcta gataatttgt atacaataca actcgcaaat cagggtgttt
     4021 gctgttagtt tgagaaaatg atgatgatta atacattcgg aatctttgac ctataataaa
     4081 taccattgtc ttcaatcatt aataatggta agagtaaaga gaattatcaa taatacagtt
     4141 aaaatcaact ttgtatacat ttcatacatt ctctcccaac aacattatac ttcttataat
     4201 ttgtatattc aatacgtttt ctgggaaaat atagatatat ctataaaaat ataaaaaagg
     4261 agttgaactt aaaatttgat taaaaaaaat caattttaag acaactcatt tgtattataa
     4321 aaatgctata tattattatt aagtaagtca atattttaga tatttatcta tttcttattg
     4381 acttacgctt atagtctaac ttatttgttt agttgatata taacctagtt ttaagcatta
     4441 ttaatattta aataatcaag tatccttttt gtttcttgta gtttctgttc tgcctcattt
     4501 aagttttctt gaattataga aatatatatt atatctatca acgtaagcat tgctattctt
     4561 gaagaaatag ctccttctct aagccttttt tctatatttg gtactactag ctttaaatca
     4621 ctaatcttac taagatgatt gtcactatat ttagttatac aaatgacctt acatcctttt
     4681 aattttgcat tttctataga ctttattact tcttttgatt ctccagagta agatattgct
     4741 atagctatat cttctttatc tacatttgaa gataccataa gttgtgtatg actatctaaa
     4801 gaggtaaatg cctgtttatt tatccttaaa agtttcatct gtaaatctag tgcaactaaa
     4861 gccgaaccac ctactccaaa tatatataca tttttagcct ttattatctg ttcaactgct
     4921 tcttttatta tatctttttc attcaacaac cttgtttggt tgatagtctc tatgtttttg
     4981 tttgtaattt tgtttataac actatctata ttacaatcat agttttcatc tacatatgtg
     5041 tattcatttt ctttgcccat caaatcttta gctatctcaa tttttaaatc cccaaaaccc
     5101 gaatatccca tttttctaga aaacctaact atacttgctg tacttgtttt tgagtttgac
     5161 gccaattctg atacagaaag cttacttata gcctctgtat tattaagtat gtaatcagct
     5221 atttttttat ctacttccgt taaatcttca tatatctgtt ttaattttaa aatgcaactc
     5281 atactctatc acctctacct atttactata atatttcttc ttagcaagta aacatgctcc
     5341 taaatctgga gtcaacactg gtctcactat accaaatctc gttttattca aatatttttt
     5401 aagtggctct aatattaaat ctcctgactt aaacactcca cctgaataag atacctttat
     5461 ctttgtacct tgttcaaaat gatgctctaa tcctattata agcctactta attcataagc
     5521 tgcatcatca aatattttac tagcagtatt atcaccttca ataactgctt ttaaacatat
     5581 cgtagcaaac tttgctattt ctattctatc tccttgtatt tcatcattta catattttat
     5641 tatgtcataa tcattctcta tattaagatg tctttttatt aaatcatata attttgtttt
     5701 tgaacacctt ccatcagact gcattgtgaa catttttaag gtagccattc ctatataata
     5761 cgctgaacct tcatctccta atacatagcc ccatcctcca catctgtgaa gattcccatc
     5821 tttatctaaa gcctgtgcta ttgaaccagt acctgcaatt atatttatac ctttttcccc
     5881 atttaaagaa cctgctaatg ctatttctac atcattacct agagtataat ttatatgact
     5941 atatgcattt tttgttgcta cttctaatgc atacaatact tcttttattt ttccatatcc
     6001 agcaagtcca acaaatgcat acgttatttc ttctacattt atcttagcat ctttacaaac
     6061 ttcttccaac ccagttatta ataatttcgt tagattatca aatcctattt gattataatg
     6121 acaagttcct ttggttatgg ttcctactat atttaattct tcatctacta atgtaaatgt
     6181 tgttttcgtt cctccaccat ctacacctaa aaaatacata ctatcctcct acaatatctt
     6241 taataattct ttcaatactt aatatctaaa aataaccttc caatgctgtt tttttaatat
     6301 tgaaaggtta tcagtactat tatttatttt acaatttttc tattgatttt aaagcttcac
     6361 ttatataacc ttcttgtcta tctaggatac tctttgcact atccttatct aaaccacttt
     6421 ttatcataaa tatagccaat ttaacgctat tatctgcttc ttctaagaat ttttctgcaa
     6481 cgtcatattt tacatttgta gctatcatta ttattctttt tgctctttca accaactttt
     6541 catttgatgt cttaacatct accatcaagt ttgagtatac ttttcccatc ttaaccatag
     6601 aagctgttgt taacatatta cataccattt tttgagctgt accacttttc attctagttg
     6661 accccattat tacttctgcc cctactataa tgtttattgg taaatccgct tttttactca
     6721 tttcactatt cggattcata gttattgata gtactgcaca ccccaattcc ttagcatact
     6781 ccattgcacc tataacataa ggagtccttc ctgatgcagc tataccacat ataacatcct
     6841 tagatgtaac tcctctatct attatatctt gctttccaag ttctttatca tcttcagcac
     6901 cttctattgc tttaaatatt gcttgatttc caccagctat tatagcttga acaagctcaa
     6961 aatccactcc gtaagttgga ggacattctg tggcatctag tattcctatc ctcccactgg
     7021 tacctgctcc tatatagaat aatctacctc cctgtttcat tctatctact attatattaa
     7081 cagcttttgc tatactctcc ttttgttctt caacagcata ggctacagtt ttatcttcgt
     7141 tatttattaa ttctattatg cctaatgtat cttctttatc tatttgcaat gtattcttat
     7201 ttctaccttc tgtaactaaa ttttctaaag tttttattaa catatcattt cctcattttc
     7261 ttaatattaa atttttaaac acattttaat tccaactttc ataattatat ttttataata
     7321 aattaatatt ttcataataa attattttgt tgagggttta aaattaaact tttgccatgg
     7381 tttaatataa tctaatataa ataattcttc ttttcttata tatcctacta cattcgattt
     7441 accactgttt ttcatatcag tcaaggctat ttgtaactcc cctgcataat gaccatattc
     7501 actactttca attactatat ctcctctttt tattatttta ggagtattaa atagttcaaa
     7561 tttgtatcct ttatatttaa cacgagattg agtagacctt atcagactat cagatatgtc
     7621 ccctctatta aaatgtattt cttcaaagag tatcttcttc tcaacttctc gtatattttc
     7681 ctctattgct acatcaaatg taaccatatc ttttctcata gaacctaata cctttagctc
     7741 ctcatatgtt ggataacaat ttgatattat tatatcattt atattattca tagctattat
     7801 atgctttact tgaatatcta taggtagatt tctgtgcatc tcaattgttg gaagcccatc
     7861 agttacaggc catggtccaa atgtagtttc attttgactt gttacaaaaa ctgctgttct
     7921 taatccatac ttattaaatc tttctgtaca ttttataaag tgttctaaat ttaatccact
     7981 atatctatgt ggataaaagt tatgacatcc tatcaaattt tctttatttg gttggtagtc
     8041 cattattgta tctatataat tagtattatt actcatattg atttctattt taagattttg
     8101 aggattaaaa gtcattaatg cctcttcaga acctgtaaat cccatatcaa gccttattcc
     8161 gtctgccttc gtctttttaa aaaaattcaa atctttgtag cttatttcta attcaccaaa
     8221 tactctggga tttacatcaa gtattacctc aaaaccttta aacttagcgt attcatttat
     8281 acttttaaat tttttaatta tattttcttt agtatcttta actgataata aacatgaaaa
     8341 tatacgtgta aatccatttt cagcagcttt atctatataa tcatatattt ccttagatgt
     8401 tgttttttct ggataaattg atattcctaa tcttcccatc tgtgtcatct ccttatatcc
     8461 tatatgtgtt taaatttatt tcgaattaca cgaaactttt ttaaaaattt gatgaaaaaa
     8521 agtttcatat atatttataa aaaaagaaaa gtttaccttt tcttttttta taaacaatca
     8581 acaaaatatc ttcgttactt atttcataac tgttatactt tgaaatttat actaatctaa
     8641 tatagttttt aatttccaat atagtttcta attaataagc ttcttgctct tcaacttttt
     8701 cttcatgaag caacctctta tcgtacatct tgacaaatgg atagtaaatt attattgata
     8761 tagctataca taataagtta agaactgaag ctctccaatc tccaccagtg gctaaatatg
     8821 cccctactgg tcctggtaat gtccaaggag cagtaactac tactctggat actaaattag
     8881 cactcatagc aaaccatgaa actgttgcca ttactattgg tgccacaaca aatggtatta
     8941 ttaatattgg gtttaatact attggtgctc caaatattat tggttcattt atattgaata
     9001 ttgctggtat tacagttgct tttcctaaag actttccata agctgatttt gctctaaatg
     9061 caagaagtaa agctaagcct attgtagctc cagaacctcc aatccatata aaccattgaa
     9121 aaaagggttc agcagcaata cctggaactg cttgacccgc agcatatgaa gctgtattag
     9181 catctaataa ttgtatccat aaaggtcttg ccaatgaacc tattattgat acaccatgta
     9241 ttccaaatga ccaaaaaaac gttattaaaa atattagtat aattactgat ggtaatgtgt
     9301 cagtggctga aactaatgga gttactaatt tagctatgaa gccatgccaa tcaaacccta
     9361 ggaaataagt aacaatagcc attaaaatta ttattaatgc ggctggtgtc agagcttcaa
     9421 aagaccttgt gactgatgct ggaactgctt ctggcatggt tatcttgaat ttagatttgt
     9481 cagtaaatct ataaatctca acacctatta tagatgttac aatagcaaca aacattgaac
     9541 ctccaccaag attagccatt ggcatttgcc atccttgagg aattgaatta taccaagcta
     9601 aaagttctgg actcttttca actatcttta ataaatcagg ggtaagaccc tctattgcag
     9661 taggaactat tgtcaaaagg aatgtagctg tagctaataa acctcctgtc aatccatcta
     9721 acttatatga ctttgccaaa ctataaccta tgccaaatgt agaatatact gatataataa
     9781 aaaatgtcat tctatatggt agtaatattg taactgcatt ttcctttaga tactgagtta
     9841 ccccccatga atctggtaat ggaggaaatg ctaatatcaa gaaaaatgac ccaactatta
     9901 taagtggtaa tgaagctacg attccatctc ttacagctct taagtgacgt tgttctgcca
     9961 atctggccat agaagttgat aatttagtgt ctaaaaattc tgtaaattta tctagcatct
    10021 tttctctcct taattaatat ttatttttat tatcattaaa atttttataa aattaacttt
    10081 cttctaacct aacaacactg caactactat ttactcaaca gctcttgaac ttgttttaat
    10141 aactttggtc ctcctaatgg actataagct tgtgcttgta ttaatgcaca tggtacacta
    10201 acctcatcag caaaagcttt taaatagtca aatctatgtc tgacttgtgg agctaccata
    10261 gcaatatccc atccattttt aacttcatcc tcaaactctt gagtaccaac agccaaaaca
    10321 tctatatcta ttccattttt tgctccttct ttttgtaatg cattaactgc tatagcactt
    10381 gacatccctg atgaacaaac aaataatact ctcatattga ttccccctgt ttttttattt
    10441 cttttcaatc attatagcat gtatttttga ttacaggaat acgtttgtat atattttttc
    10501 atattttaca tttttatttg taattttatt tcatgaattt atatcttgtt ttaattgctt
    10561 tatttatcta atcattcttt tgtctaaaat tacacactac ttaaaatact catatctttt
    10621 cttaaaaaga aaaaagacta tcttattatt aacataaaat agtctcaata cttttttaaa
    10681 tatgtattcc tttatatatc cagaagtaac ctattattgc aaatgataaa gcaaataaaa
    10741 tatacagtct aaaaaatccc aaagaaaaac ctctcttagt ttcaacaaag tatcttgtat
    10801 atgcaactgc aaaagatgta attaatgaat ttcctataac aactccaaat ctattatcta
    10861 ttccaaatat agataataac aaaggaaaaa ctacagtact tacaagagat gaaactatta
    10921 gtattaatcc acttttaaaa cttatagttg gaatattata atctgttctt tgatttttct
    10981 ccatttgttt cttagtattt ttattacttt ttgtactttt tgcattttta taattattat
    11041 ttttctttct tttctttttt ttattattag acatgtcatt tacctccaaa aggtttaggt
    11101 gttatattcc ctaaaataac tttattttta gcccctgttg cagatattac attagaaaaa
    11161 gaattattta atgtttcatt gcccaagatt acaaatgcca aagcctcctt tgcatcagaa
    11221 gaatacccat attcatcttg tgttaatact tcaacctcta ataataactc ttttatatat
    11281 cctattaaag ttaaattatg agctcctcca cctccaatta ataaagtatc tacattaacc
    11341 tttgaaagta tgaagttttt ataattataa tatatagctt ttgccgtaaa catagttact
    11401 gttgctatca aatcatattt atctacatgt ttatatttgt taagcatgtt atccaaatag
    11461 acttgtccaa atacctctct tccagtgctt ttaggtggtg cttgactaag atacttatga
    11521 ctcatcaaat cttttaacat ttcttcatta attttacctt ttgatgcaaa gtatccattt
    11581 ttatcatact ttctattaaa taatctttga catactccat ctattatcat atttcctggt
    11641 cctgtatcaa atgcaaatac atcatctatg ttacatgatt taggtattat agtaatattt
    11701 cctattccac caatattttg aagtgctaaa tttttattgt tatcactata taataaaaat
    11761 tcactatatg gtactaatgg agcaccttct ccacctgctg ctatatccat tactctaaaa
    11821 ttagatatta cctttgtatt tgtttcatat gctataactg ctggttcacc tatttgaagc
    11881 gttgaagata caaaattatt ataacttctt ggtatatgaa agattgtttg accatgagat
    11941 gcgataaaat ccaaatttgc tatatgaaag tttgctttat gacacactga ttttacagct
    12001 ttactaaata aatatccaag cttaaaattt aagctacaga ttaaatcaac actcgattcc
    12061 tcttctatac agcactttct tatttcgtct tttatatctt taggaatttc tagagttata
    12121 aattctattt gcttaacttt agtatttcta ccattcccac taatttcagc tagtactgca
    12181 tcaactccat ctaaggaagt tcctgacatt aaacctacac tatacatagt tatcatcctt
    12241 ttaagtttat agttattcct ccccaaatca aggtattaag tttaataatt ttagaattaa
    12301 tcttatttca tctttaatta cgataattta ctcttatgaa attaattgag ttaatcttat
    12361 acacgttttt taccttagct aaatcttaca ttaatttatt ttcagtgtca atgtaatacc
    12421 atgtaatttt ttttcacaaa caaaatatat tctctctcca aaaaccatat taaaatagtt
    12481 actacaaata aaaaacatac caggtaccta tctgatatgt ttttcatata taataattat
    12541 tattttataa tatagataga gcatcattta aggcactaat taaatcttca ctattttcaa
    12601 gaactaaatt tattcttatt gtatatgtcg atttgtatat gatgatagat tttatagtct
    12661 aataacctaa ctatctaggc ttaaataatc cgatatatca taaaagtaga tattaaaaat
    12721 tgtatatgaa ccaattttcg aacatacctc aatctcatgc cctaaactat ctatcatttt
    12781 ctttacaaaa tacatcccca ttccagtaga cttatacaat tgatttcttc catttttccc
    12841 cgtaaatccc ttgtcaaaaa ccctagatat atcttcctca tttataccta tgccaaaatc
    12901 ttttatagat aataccactc cattttctaa cttttttgaa ttaaattcaa tcttaccaac
    12961 ttcctttgaa tactttatag cattattaat aatttggtct aaaacatatg acatgcattt
    13021 agaatctgtg taaacatcat tttctaattc attcaaacta acttctacat tattttttat
    13081 aagcaaaaat gaattatttt ttatagacat ttttaccaat ttttctaaat taaatttatt
    13141 tataaataca tcttcaaaca tagtgttgct tctactacta tacaaaatag agtttactaa
    13201 aaaatttatc tttgcaacct gattcttaat atccaaacta tcttctatgc tacttaacct
    13261 atctgtaatt atacttaaag aagatattgg aatttttatt tcatgaaccc atttagctat
    13321 gtactcatcc aattctttta atgattgttt ataagtctct atgtcactat tattttttat
    13381 ttttaagttt tctataatat cttttattag attttcttca aaagattgtc cattaactaa
    13441 ataactgtct atttcaccac tattttctat acaattatat aaatttgtgt aactatttct
    13501 aaaatttata ta
//
LOCUS       DAGSWI010000111        13371 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.111, whole genome shotgun sequence.
ACCESSION   DAGSWI010000111 DAGSWI010000000
VERSION     DAGSWI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.111"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            241..2253
                     /locus_tag="MJF80_002892"
     CDS             241..2253
                     /locus_tag="MJF80_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0281541.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNRKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            2622..3842
                     /locus_tag="MJF80_002893"
     CDS             2622..3842
                     /locus_tag="MJF80_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0281542.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKVDYIFGLHVMPHIETGFIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            3879..5228
                     /locus_tag="MJF80_002894"
     CDS             3879..5228
                     /locus_tag="MJF80_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBZ0281543.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            5342..5904
                     /locus_tag="MJF80_002895"
                     /pseudo
     CDS             5342..5904
                     /locus_tag="MJF80_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6012..8345)
                     /locus_tag="MJF80_002896"
     CDS             complement(6012..8345)
                     /locus_tag="MJF80_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBZ0281544.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSSLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(8342..9025)
                     /locus_tag="MJF80_002897"
     CDS             complement(8342..9025)
                     /locus_tag="MJF80_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281545.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(9096..10337)
                     /locus_tag="MJF80_002898"
     CDS             complement(9096..10337)
                     /locus_tag="MJF80_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0281546.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            complement(10348..11034)
                     /locus_tag="MJF80_002899"
     CDS             complement(10348..11034)
                     /locus_tag="MJF80_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281547.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKILLTVR
                     RMGYKWNVIK"
     gene            complement(11272..11616)
                     /locus_tag="MJF80_002900"
     CDS             complement(11272..11616)
                     /locus_tag="MJF80_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281548.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKQLTVTGNEYAIINY
                     YMSNFSQYIIFAILLASMRFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
     gene            complement(11947..>13371)
                     /locus_tag="MJF80_002901"
     CDS             complement(11947..>13371)
                     /locus_tag="MJF80_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281549.1"
                     /translation="LSLGTIIISVSKPLMLSVKTDPMICIRRNNEKVDIKQNSFINKF
                     MIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKSTSNFLSDGVLKYWI
                     QGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKWFNIKIDDKSINKN
                     SDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLINGKENIDKFSKQPY
                     LYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCGIITTLPVTSQVGA
                     EFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITEKTNKGILIPYKAE
                     TAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREFALFRGIGISKHEI
                     NKIVKLESYIYIIVSFCISIIPILIVRSVIIKPFESINLINWKFIGAIILIVFILVSI
                     IMITTLKTLNKIQNENFMEEIKTL"
BASE COUNT         4870 a         1880 c         1652 g         4969 t
ORIGIN      
        1 gtgtatatta taatttaatc gaatagaaat gaaaaatata catacagttt aagttttaag
       61 caacaattag ggggatttgg tgacaagatt tttaattata agaaagaaag tgtcttaatg
      121 caacttttat aaagtcatta tagacacttt cttttttatt tgataaattt aaattatcaa
      181 aattatattg ctttatacaa ctattttgtt ggaaactatt tattatataa ggagaaataa
      241 atgaaaaaat cagtagctct ggtaaatgat agtagaaaag atttaataga ctttttagaa
      301 aataatttga aattagtttt tggagattct ataaatatta atagatactt cataaatgaa
      361 ataaatgata atgatattat aaatgatgat gtgatattag taatgtcagt agaaagatta
      421 gacaaaatta taaataatat actagataag aaaaaagtga ttgtggtaag aagaactttt
      481 agggaagata aaatttataa tctactatct ttaccacagg gtacaaacgt tctaatcgta
      541 aatgattctg atgaaactac tctagaaact ataagtttat tttataaaat tggagtgact
      601 aatattagac caattccata tatgaatgat aataactata aaaatataaa aatagctata
      661 actccaggag ttccagaaaa agtacctagc tttatctcag atatatttga tttaggtcac
      721 agatatatag atatctctac atttatagaa ataataaatt tattacaaat tgattcaaag
      781 gaaatacaat ctaatctagt taaatactca gaagaaataa taagtttaga tactggtata
      841 aaagataaat ataaggaatt atttttaaaa atagaagaat tagatacaat cttgaattta
      901 tcaaaagatg gtatattatt tacttcaaaa gatggtgaaa taaatacata taatagtaaa
      961 gtaaaagata ttttagacat aaatgaggat atatatggaa aatacattga ggatatattt
     1021 gtagatagtt taaaagtttt attgagtgag aaagagatac tagataaagt tgtcgtattc
     1081 aataggaaat atataaatgt aaataaaaaa aacatataca atagagatga aaaaatggga
     1141 acatattata gtttacaaga aataacatac ataaaaaaat tagaacaaaa tcttactaaa
     1201 aagttaagag aaaaaggaca aatagcaaaa tatacattta aggatataaa aacaaatagt
     1261 cctaaaatgt ttgaatgtat agatttagct aagaaggttt ctaaatctga cttaagcata
     1321 cttatcagag gtgaaagtgg aactggtaaa gaactcatag ctcagtctat acataacaac
     1381 tcaaatagaa aaaatcaacc ctttatagca gtaaattgtg cagctgttcc tgaaaattta
     1441 ttagaaagtc agctattcgg atatgacaaa ggaactttta ctggaggtct aaaagatgga
     1501 aaacaaggat tatttgaact tgctaataat ggaacaatat tcttagatga aataggcgat
     1561 atgcctttag aattacaaac aaaactactt agagttcttc aagaaaagca gataatgcct
     1621 gttggatctc acaatgtaat taatatagat gttagaatca tatctgctac aaataagaat
     1681 ttagaacaaa tgattgataa tagccaattt agagaggatt tatattatcg attaaataca
     1741 attccaataa atatacctcc tctaagagaa agaaaagaag acatattaat tattatggaa
     1801 gatttaatta ataagaaatt agtaatcaca ccagaagcaa aaaaacttat acaaaactat
     1861 atgtggaaag gtaatataag agaactccaa aatgttacat cctatttaaa cattatgtgt
     1921 gaagatattg tattagagaa agatttacct ccaaacttaa ggtcttcaga taacaaaaat
     1981 acatcattaa aactaaaata tagtaagaat gatatattga atatattaga gatactaatt
     2041 ttaaataagg aatcagatgt tggtattggt agaggattaa ttttaaaagc actactcgat
     2101 aagaaccttc aaattacaga aggaaaaatt aaaaaaatat ttgaatatct aaaaaaagaa
     2161 gaacttataa tttgtagttc aggaagatat ggaagtaaaa taactcaaaa aggagaagat
     2221 ttttacaata aattaaaata taaaggtcta taaaattata atttatcaaa caaagaaatc
     2281 tattcaggat taaagtactc cttcaatagg tttttttatt tttcaagtac cctaaaatta
     2341 taatttattt tgtacataaa taataggaat taataggaat aggataattt ttttatttcc
     2401 tatttttttt atataaacta gcatataaaa tatattttct tcaaaataat attttttact
     2461 gaaaattgaa actatgcaat cattttttat actatacata tagacaaatg attatattta
     2521 ttcaatattc ttataaaaaa caaaattctc catcgaaaat tatggcataa taattgctta
     2581 ttatattaat aaaacaataa aaagcaattg gaggaataaa aatgcaacag gctttgaaat
     2641 taaaatatca aaccaaagaa ttaggaaaag attttgtaat agaaagttgt aattctataa
     2701 aaccatggct tataaacata agaagagaat tacataaaat tccagaatta gctcttgaag
     2761 aaaatcttac taaacagaag gtaatttctt atttaaaaga aatcggaatt gattatatgg
     2821 aatttacaaa acacaatgga attatggcat atattttaaa agaaagtgca gataaaacga
     2881 tttgtataag agcagatatg gatgctcttc ctattgaaga agaaaacaac ataccttata
     2941 aatcaataca ttctggaaaa atgcatgctt gtggccatga tgcacataca accatgctac
     3001 ttggagcatg taaagtttta cattcaataa aagataaact taatgtaaat gtaaaattct
     3061 tatttcaacc agcagaagaa ggttttggag gagcaaaatt cttagttgaa gatggatgtt
     3121 tagaaaatcc taaggtcgat tatatattcg gacttcatgt tatgccacat atcgaaacag
     3181 gatttatcga aacaaaatat gatacattaa atgctagtgt agacactata aaaataagta
     3241 tcaaaggtaa aagagctcat ggtgcatatc ctgaaaatgg tattgatgca atagttacag
     3301 cttcacaaat tgttacatca ttacaaacta taataagtag aaacttagaa ccaaataatg
     3361 ctgctgtttt gactataggt aaaatatatg gtggtgatgc acacaatgta atatgtgaag
     3421 atgtaaaact agaaggcact ttaagaacct taaatagcaa aacaagaaac tttatgattg
     3481 ataaaatagc taaaattgtt ggagacactg catctgcatt tggttgtgtt gggactttac
     3541 atgtaagtga tgaaaactat cctgctgtta taaatgaaaa agaattagta gatacagtta
     3601 tatcaaatac taaagaatta cttggagagg aaaaatttat attgagacca aacccgtctt
     3661 taggtggtga agacttttct ttttacacag aacattgtaa aggagcattc ttccatttag
     3721 gatgtaaaaa tgaggaaaag ggcttaattt cacctcttca tacctctagc tttaatatag
     3781 atgaagattg tttaccaata ggtgttatga tgcatgtaat gaatactttg tattttaatt
     3841 agtacagtaa ttaataaaag aataggagat aaattaatat ggaaaaagct aagaaattag
     3901 gtctatttag tatgatatta ttagggatta atagcataat aggttcaggt atattcttac
     3961 taccaggtaa agtttataat ctagcaggtc aaaacagtat gtttatatac atttttgcta
     4021 cactcttagt tctatcaata cttttatgtt ttgcagaagt tggaagcatg tttgataaga
     4081 atggaggagc atatctatac tccaaaaaag cttttggaga ttttatagga tttgaagttg
     4141 gtacaatgtc ttgggttatt agaattatat catggtctac tctagcagta ggatttgcaa
     4201 cagctttagg ttcattttgg ccagaatctg ccactgaata caaaggttat atagctgcaa
     4261 tactcgtaac actactttca ataaatagct tatttggaat taaaagcaca aagataatga
     4321 ataatgtaat aactatagct aaattagtac cattaatagt ttttataata gttggaatat
     4381 tcttcataaa atttgtaaat attgttcctt caggaaatgt agttaactct agtatgggtc
     4441 cagccattat attagtattt tacgcattta ccggctttga atcatttatt gtggcaagtg
     4501 gagaaatgga aaatcctaaa aaaaatcttc cagtcgcatt aataaccaca atatttatat
     4561 gtgcaataat ttatatatta attcaaattg tctgtatggg tatacttgga gatagactat
     4621 ttgaaaacag tattccaatt gcagatgctt caagtgtatt tttaggcaat tatggtaaag
     4681 ttttcatatc tgtagcaaca ttaatatcta tatttggtat aaatataggt tcatcaatag
     4741 taactcctaa atgtggttct tctctagcag aagaaggttc tcttcctgca tttataggaa
     4801 aaacaaataa atatggtgct ccatatgtag caataataat atctttaatt tgttgtatac
     4861 cacttgtgct aactggaagt tttgaacaac ttgcagtaat gagtgtaatt gcaagatttg
     4921 ctcagtacat accaacttgt ttatcagtaa tagttttaag aaaaagaaca gatgttaagg
     4981 cctcttttaa gattccattt ggtcctgtta taccaatggt agctatttta ggtagcttat
     5041 ggttgttaca acaagcttgg gcagaagata tttcaaaacc tataactcaa aatagagttt
     5101 taataggatt aggggcaatg ttgttaatag ctcctctata tatatttatg aaaaaaaata
     5161 aggaaatcga gcataaaaat aaatcagaag attctaaatt atatatttcg agagaaaaaa
     5221 ttcaataata ttttacaaac tatttaaaaa ttatttttct aatactatag aaataattag
     5281 aaatggagat gttacgtatc taaaagtagt tgataacacg gatattggta cttttataga
     5341 tatgggactt acaaaagaca tactagttcc ttttaaagct aagacttatc cattgtttag
     5401 agatgaaaaa tatttattct atatatactt ggataaaagc agaagaatag ctgctactac
     5461 agacatagat tcatatttag aaacagacca tacttacaat gtaggagatg ttgtaaatgg
     5521 agttgtatat ggattccaaa ccaacaactc tgctatgata tgtgttgaca ataaatatgc
     5581 tggtgtaatc cttcataatg aatactttac agaattaaaa gctggggatg ttcttgaaaa
     5641 tttacatgtt ataaaaatat acgatgatgg taaattagga ctttctccta gaggaaacag
     5701 aaaagatgaa cttgatacct tagaaaataa gattttaagc tatttagaag gttcagatgg
     5761 atatatgaga ttcaatgata aatctgatcc taaagatatt tctatcttat ttaattcaag
     5821 taagaaaatt ttaaacgtgc tcttggagta cttatgaaga aagacttaat ttatcaagat
     5881 gaagatggaa cttatctaaa gtaaattata taacacaaaa atcgaagtaa attatgaaag
     5941 atattttcta tttcatattt acttcgattt tttatttaaa ttactacctc tatttaaatt
     6001 actacttata tttattgtgc atttactacc tctattatgt ctaattcaca aatcttttta
     6061 attggttttc ttactgcaag caaagttatt attatcatag ttactacaat gataaacagt
     6121 aaatcaaaag gtatttgcca gtctatatgg aattttaatg ttactaagga tacaaatata
     6181 tatctatgta atattaatcc aagtacactc cccaataaac agccacttac agcatatgta
     6241 cttgattcta ctattaccat ttttcttaat tgcttattgg acatgcctat tgcacgcata
     6301 ataccatagt aatttattct acttgtaacg cttatattca tactgtttat gatattaaac
     6361 atacttataa tcgcaattat aaatacaaac ccatatacaa aaactgccat agtgtaaaat
     6421 gaatttttag cctctaagtt accttgtcgc ttatctcgaa cctctaaatt atcatctaga
     6481 acatttctta tattttggat taaatcttca ggagcatctt catcaacttg agtatcaatt
     6541 attatatagt cttttatatc tgttatacta gtaaaagtat tttcagatgt tataatattt
     6601 ccaatatatt tgtctttaac atcaaatgaa attgaagaaa gtatacctgc tacctctatc
     6661 tctttcttag aatttccatt tttaatatat atcttatctc caatattaaa cccatgacct
     6721 tcttctacca atccgatatt ttctgactct aatttatctt cattgatttc cccttttact
     6781 agatactttt tggcccagtc aaattgattt ttttcataag aaattagctg agatgttgcc
     6841 tccttattat tgtatgtagc ctctacatcc gtcatcattc tactgtatac tttttttatt
     6901 cctttgatat tacttatttc attaatatcg tctttagtaa gaacagactt aaagtcagcg
     6961 tcaacaatag atatatgagc agcttctggt tttataggtt taagtgcctg atacataaag
     7021 tctataaata cactaaaacc taaaaacaat acaatactaa tagcaaatga agaagtcata
     7081 agtataaaag atttttttct tgaaaaggca tgatttattc ccatagaaac atcaatttta
     7141 gaacttcctg cgttagcaat tttacttgct tttccactag ttaaaatttc tttgaacatt
     7201 agattaccac gtatagcact tagtggagac accccagaag ctttttttgc tggaacaata
     7261 gctgataata ttactgttaa aaatcctact attattccaa acacaatacc aataaagcta
     7321 acctttaaaa ttggcatcga accaaataac tctgtattca catatttgag aaatatagag
     7381 gatataatgg ttacaataca tcctaataac aatcctagtg gaatagcctt tacacttaga
     7441 attaaacttt ctaaaacaac aaattttttg acttggagtt ttgatgctcc caagcaacgt
     7501 aatagtccaa aaaactttat tctctctatt acacccatat taaagctatt atatatcatg
     7561 ataatacttg ctattaacac aagtataaaa agtcctcctg ctgttaagta aagtggttta
     7621 gaataatcag agtcaccttg acctataata gaaagtaatt tttcattttt accgatttgt
     7681 ttatcactta aattgaaggt attcttaata tcagaagtag cttttctaat attaaccttg
     7741 tctttaaatg taatataata ataatcatat gtttctgatt ttttgatagc ttgtgtacct
     7801 aatattgaaa gcattaatcc atgcttatct gaggctctta agcttgataa tgatgaatat
     7861 tcacctacta tcttataatt tttatttcta ccatctgcta attttatttt gataaaatct
     7921 cctactttta gattaaagct tttaactgca tctacatcta ttaaagtctc attctccaca
     7981 acaggataac gaccttttat tatttctaga cctaattgtt ttgctaaatc ttcatctaaa
     8041 ccttgtatca aaatctcctt tgatttaaac tctccattat caatgacttg tcctctacca
     8101 tagtgagcta tatctactct actacttatt tctgatatag cttcttcatt aagattttta
     8161 agtactatgt gccaatttcc atcatctttt ataaattgta ctttttgaca ttgtatagcc
     8221 atgtctgcta gtccaaaaat agcagtaact aaacatactg caatgacaat acataaaatt
     8281 gtagttctat tttttttctt atgtaccttt gcatattgtg gtactagttc taaatagctt
     8341 ctcattctct attcacccct aggtctttaa gcattccatc agaaacctgt agtatcctat
     8401 ctgctgtaga agctatattt gtattatgag ttatcataat aatagtttgg ttatattttt
     8461 ctgctgtcac ttttaaaagt gaaattacat cactgctatt tttactgtca agatttcctg
     8521 ttggctcatc tgccaatata attgatggct ttgtaatcaa tgctcttcca attgcaaccc
     8581 tttgttgctg accaccagat aattgatttg gaagatgact ttttctatct ttaagaccta
     8641 aaattgtcaa tacttcatct agataaatag tgtctggttt tttgtaatct agtagtaaag
     8701 gaaatattat attttgttcc acagttaatt cgggtataag attatatgat tgaaagataa
     8761 aacctatatt acgacgtcga aaaatagtct gtttttcttc tttcattgaa catatttctt
     8821 taccatcaac aaatattttt cctgaggttg gagtatcaag acctcctaaa agatttaata
     8881 atgtactctt acctgagcca gattctccaa ctacagtaac aaactctccc tttgaaactg
     8941 taaatgatac atcttttaat gcctctactt ttgtatatcc atttccatat gttttacata
     9001 aattattaac cacaagaata tccatttgct ctatctcctt tctgatgatt aatataagaa
     9061 taattcatac accttactgt aagatgtatc ctatcttaca tattagtaag ctttaaaaaa
     9121 cttagagtaa atgtacttcc ttgtaaaaaa ttactttcta cagttatggt tccattgtgc
     9181 tgttctatga tagatttaac tagtggcaac cctaatccaa ggccttgatt actttttgaa
     9241 aacttactcc tataaaatct tttaaatata tgatgtatat cttcaatatg aattccattt
     9301 ccggtatcac tcactgaaat aatagttgtt ataggtgttt caacccattc tatcattatt
     9361 ttatcgttct cttttgtatt atctaatgca ttttttataa ggttgcttaa agcttctgat
     9421 atccattcat aatcgcaaaa caagtttgta ttttctagac ctcttataga gatactcttt
     9481 ccttcttttt ctgctctagt ttcaaaacta tataaaattt tagtaatcaa cttttttacg
     9541 ttaacactag ctttgttaag aattattgtc tttgaatcta tctttgtaat ctttaacaag
     9601 ttttgtatca atgattctat acgctctaat tcattttgac tcttctgcac aaaattctca
     9661 acaatttcac tattatgact ctcttcctgc aatatttgat tatacatcat caatgctgta
     9721 agtggagttt ttagttgatg tgatatatct gaaattgtat ttttaagaaa ttctttattc
     9781 tgcttttctt tcaatatatg tgtgtttaga gatgttgaca tagcatctat tgaaccaaat
     9841 aactttgata aactaccttc ttcataagta tttaaccgta catttatatt tccattcata
     9901 aatgaatcaa ttttataact agcttcttct atcttgttct gttgcctaaa aaaatacaca
     9961 tacaatataa aaataatcat agtaaagaac acaaataaat acaaaaacat agtttttata
    10021 tatttacctt gaaacaattt cacacttggc ataaaactat catctgtacc aaagtcatat
    10081 ctagctgtat ttaagatttc ttttcctttt aaagtatatt tatcttcatt agtagtaaat
    10141 actgctggta tttttgattt atcaacaccc atactattta agtatccagc tatattataa
    10201 tcatgttcta acagtgcact tttataatca ttagttatat ttataactac tatttgacct
    10261 actactataa acatcaataa aacggatata atcaccaaaa aaaatatttt tatgtcttta
    10321 ttagaaaata ccttcatatt aacaccctca ctttataaca ttccatttgt aacccattct
    10381 tctaactgtt aatagaattt ttggattttc aggattatct tcaatcttat ttcttaacct
    10441 tcttacatat actgacagag tattatcatc tacaaaacta ccatttccat cccataactt
    10501 atccaatata ttttttcttg taagtacaat atttttattt ttcataagta agcataataa
    10561 tttatattct gctgtagtaa gctcaagctc aatttcattt ttaaaaacac gattttcaag
    10621 taatttaaca gtaatattat ttgattttag ttcagttaca ttttcacttg taaaattata
    10681 acgtctaagt aaagctttta ttcttgaaat taactcattt aatttaaaag gttttgttat
    10741 gtaatcatct ccacccatat caagtcccat cactacattt acttcttcat cagaagcagt
    10801 taaaaatatg attggtacat tagaagcttc tcttaccctt ttacaaatat caaaaccatc
    10861 accatcaggt aaggatacat ccaaaagcag taaatcataa tttttttctt tataaatttg
    10921 caatgcttct tctactgttc ttgctacatc aacactaaat ccattctttt gtatagaata
    10981 ttctaatcca tatatcaaac ttaaatcatc ttcaaccaac aatatactac tcatctcaat
    11041 cacttccatt tttttatttt ttactttata tatattatat acctaactta ttatattttt
    11101 actcttttaa aaatacaata ccttgaaatc aatgatttaa atattttaaa attattttta
    11161 tatgatttta tatagttaat ttaatacttt gaaatcttaa taataaaaaa agcttaaaat
    11221 acactaagta ttctaagctt tattcaatca cattttatat atttatatta tttatttttt
    11281 taaaatacta ttttcaatat tgcctctatt aaacatttca ttatcattat ctaaagagat
    11341 atcacttata tttctaacag ttgttgaata gttaccacta agttctatct cactgaaatg
    11401 catatgcact atcttttgta ttacaaatct cattgatgca agtaaaatag caaatattat
    11461 atactgacta aagttactca tataataatt tataatggca tattcatttc ctgtgactgt
    11521 taactgtttt gtagatagca tagtatatat atattcagta caatttataa atacatatat
    11581 accataaata gccaacacta cagataaaca atacattgtg attgaaatct ttgatatttt
    11641 tttaaactta ctatctttac aacacttaat atttgttatt tatcataaac aaggttattg
    11701 tattatcaat aatttttagg ttacacttat tattagtctt gtgttaaaac gacagtagac
    11761 ttaagagtag agttttatta agccaatatt tatatttgct atacattgta cccctattac
    11821 accatacata taacttggat aacttatttt atttttattt ctaaattatt taaaatcaat
    11881 atctacacca ttatcgataa ttgcaattaa attcatataa aacctcttta atttataggc
    11941 tgttcactat aaagtcttta tttcttccat aaaattttca ttttgaattt tattcaatgt
    12001 ttttagtgta gttatcatga ttatagatac taaaataaat actattagga taatagcccc
    12061 tataaatttc caatttatta aattgatact ttcaaaaggt tttattatta cacttcttac
    12121 aattaaaata ggtattatag atatacaaaa gcttactata atatatatat aactttccaa
    12181 tttaacaatt ttatttattt catgttttga tattcctatt cctctaaata gtgcaaattc
    12241 cctacttctg ctattaatac tagtaactat agtattgcaa cagttaaata aagataatat
    12301 aactataatt ccagttacta ataccataat tagtgttttt tgattatcaa ttttttcata
    12361 ttcagctgtt tcagctttgt atggaattaa aattccttta tttgtttttt ctgttatttt
    12421 gtttaactca ctttcaacat atttatcatt ggctagttta ctcgtccata tgtcaaatct
    12481 ttcataagat gatattcctg taaacttatt aaattgattt gttgatatta ctccgccaaa
    12541 ttcagctccc acttgtgagg ttactggtaa tgtagttata attccacata ctataaattc
    12601 ttgagatatt gttttggtaa aattatttgt ttttgaatct ataatatcaa agtatacttt
    12661 tattctatct ccttttttta tattaagcga atcactggat ttctttgtta tatacagata
    12721 tggttgttta ctaaatttat ctatattttc ctttccattt atcaagaaat cctctagtat
    12781 atctgtatct tctattccta aaaattcaaa agaattatta tataccttaa ttccatcttt
    12841 tacttctgat ctagcttcaa tattatcttt attttttttc caataatcag agttcttatt
    12901 tatactttta tcatcaattt ttatattaaa ccacttatgc ctatatgagt ttacttttgt
    12961 tacaccatca attgccttta tctcttgtag tacattttta tcataacctt tattatttga
    13021 acgtatagta cttatatcaa tattatgtaa taaataatct ccttgtatcc aatattttaa
    13081 tacaccatca cttaaaaagt tactagtaga ctttgtgtat attgttgcca ttaagaaaaa
    13141 tataattacc attgatgcta tagataaatt tgtacgtttc ttatttcttt gtatattttt
    13201 tgatgctatg ttaccatagt ctttgaaaag ttttatcata aatttattta taaaggaatt
    13261 ttgttttata tccacttttt cattatttct tcttatacaa atcattggat cagttttaac
    13321 tgataacata agaggttttg aaactgatat tattatagtt cctaaactta a
//
LOCUS       DAGSWI010000112        13322 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.112, whole genome shotgun sequence.
ACCESSION   DAGSWI010000112 DAGSWI010000000
VERSION     DAGSWI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.112"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(225..1892)
                     /locus_tag="MJF80_002902"
     CDS             complement(225..1892)
                     /locus_tag="MJF80_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBZ0281550.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(1966..2721)
                     /locus_tag="MJF80_002903"
     CDS             complement(1966..2721)
                     /locus_tag="MJF80_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBZ0281551.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EILTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            complement(2731..3267)
                     /gene="lepB"
                     /locus_tag="MJF80_002904"
     CDS             complement(2731..3267)
                     /gene="lepB"
                     /locus_tag="MJF80_002904"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBZ0281552.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(3433..3969)
                     /gene="lepB"
                     /locus_tag="MJF80_002905"
     CDS             complement(3433..3969)
                     /gene="lepB"
                     /locus_tag="MJF80_002905"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBZ0281553.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(4069..5379)
                     /locus_tag="MJF80_002906"
     CDS             complement(4069..5379)
                     /locus_tag="MJF80_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBZ0281554.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            complement(5458..6174)
                     /locus_tag="MJF80_002907"
     CDS             complement(5458..6174)
                     /locus_tag="MJF80_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBZ0281555.1"
                     /translation="MKLDLFLLAIIPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            6419..7690
                     /gene="sleC"
                     /locus_tag="MJF80_002908"
     CDS             6419..7690
                     /gene="sleC"
                     /locus_tag="MJF80_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBZ0281556.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(8116..8772)
                     /locus_tag="MJF80_002909"
     CDS             complement(8116..8772)
                     /locus_tag="MJF80_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281557.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGVRILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(9884..10294)
                     /locus_tag="MJF80_002910"
     CDS             complement(9884..10294)
                     /locus_tag="MJF80_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281558.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(10510..11892)
                     /locus_tag="MJF80_002911"
     CDS             complement(10510..11892)
                     /locus_tag="MJF80_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBZ0281559.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVILRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(11903..12262)
                     /locus_tag="MJF80_002912"
     CDS             complement(11903..12262)
                     /locus_tag="MJF80_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0281560.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(12467..13246)
                     /locus_tag="MJF80_002913"
     CDS             complement(12467..13246)
                     /locus_tag="MJF80_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBZ0281561.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         4537 a         2409 c         1319 g         5057 t
ORIGIN      
        1 aaaataatca cataaattaa caaaatatta aacaaaaaag taaaatttaa aataaaaagc
       61 ttgcttcata caaaagtata aaacaagcct ttttatatat caactcaaac tttaaaatct
      121 gtatcaattt aattctccac tttacactta atatacttaa cataaaccaa taacactact
      181 tgctatgcca tttaatcttc ataaaacaat ctataataca cacattaatc aactatttta
      241 cttcctccaa taaattccct aagaattgaa atacctggta tgtcagaagt atcgtccaat
      301 tcaacaaaat actgcaaaaa ctttagtaat ctagtagctt caatatgtgc aggattatct
      361 tcctttatct cttccaacaa aggtttcgca aatcgtttta aaactgccaa ttcatataca
      421 caagcagagt taaatatagc atctgcctcc tcttggtacg ggaatatatt cttcttttct
      481 cctcttctaa ctgatgccca ttgtaaaata gtattatcag cactatatcc tctaaaatta
      541 tagtctctaa caattcttct tatcattctc aaatcagcag tagggattcg attatggtca
      601 tccaaattta tttgagtaag ggcacttatg tatatcttaa atttatcttc atctggtata
      661 gatgatgtta aaataggatt tagcccatgt ataccttcta atataattgg ctgatttgaa
      721 tctatcttta tctttttacc agtgtattct ctcttacctt ctttaaaatt gaatcttata
      781 tcatcaattt cttcaccagc taaaagtttc tttaagtcta agttaaactt ctctaaatct
      841 attgcataaa ttgattcaaa atcataattt ccatattcat ctaaaggagt atgttttcta
      901 tctataaaat aatcatctaa agaaattgaa ataggattta aattatttac tctaagatgt
      961 attgataatc tgtgagcaaa acttgttttt ccagatgaag atggagctgc tattaaaact
     1021 actctcttat tgccatcctt tatcatatcc gctatttggg ataacttctt ttcatgcaat
     1081 gcttcaaatg tccttattat atctccatat tcattgtttt ctattatttt attaagagat
     1141 actaccttgt caattcccat aagtcttgac cattcttctg cttcataata tacacttgat
     1201 agcttaggtt gaggtacaaa tttcatagga agagtcttat cttcttcact aggtcctaaa
     1261 attataacgc ctccattata aagcttaata tcaaatgatt ttatataacc agttgatggc
     1321 aacatataac cataaaaatg gtctatatag tttctacact tatatacttt agcactatca
     1381 tactctttgt atttcaaaag ctctgccttt tcatacattt cattattttc aaagatagaa
     1441 atagcttcat ttcttgtaac ttcaattttt tctataacat aatcgttgtt tactatctct
     1501 ttcatcttgt tttttatatt ttcaacatct gcttctttaa gctttctatc tatatgaact
     1561 tcacaatata gcccatctga tagtgaatgt tctattgtaa ctctgctatc ccaaaagatt
     1621 tctctacatg ccattacaaa tataaatgac atgactctaa agtaaactct ctctccatct
     1681 tcattagtag tatctaaaaa atttatctca caatccttct taactctaca atttagctct
     1741 tttagtttat tgtctacaac tgcaagtgtt atataaccct tatgctcatc acaaaattct
     1801 tttgctatat ctcccaatct gattccaggg gaattttctt ctaatgaata ttccttatct
     1861 tttccttcta tttttaagtt tatttttttc acttcaacat ctccttcctt ttgatgaata
     1921 taaattttaa ttaatgaggc atatcaacaa gataaagaga ggtatttaac cctctcctac
     1981 aatattattc aatttactta tagtatcttc aatgtcttcg taatttattt ctaatactaa
     2041 atattctgca tctaatgtta agatctcttt aatcccctct tcagataaaa ttcctcttcc
     2101 tataccaaca tgtgtatcat tgataccatc attatcactt aagtgtatca gtcttatttt
     2161 atcctttaag ttctttacat aagcatcctc atcaattaaa cagtgtccag tatcgtaaca
     2221 aaaacaaatc ttagaatttt taatcctctt aaatatatat tcaaaatcat aagatatatt
     2281 tccaatattt gaaaaatccc caccttttga ataagtattc tctatacata catatgaatt
     2341 tgtatttaat ttatctaaaa aatccacact atttcctaaa tatttgtctc tattctttct
     2401 aactctattt gtcataacat atcccaaatg aaggttaaaa taccttaaat caaatccttt
     2461 tagttcaaat tctatcttct caataaaatt tatccatgaa tttcgtatat actctatgtt
     2521 ttcgcaagta tttaattcca taggcaaatg tatccctata gataatccta acttagaaat
     2581 tctctcttta tatttacata actcactaca ttcagatata ttgtcaattc caatctctaa
     2641 atgagttatt tgctttatac tctcacaaat gtttaaagcc tcctcaatat taaacaataa
     2701 ggacgctata ccaactgcca tacaaatcac ctactcaaaa atacctatat ctgtaaaagg
     2761 aaatactctt aaaataactt ttcctttaat attttcttca tctactaatc ccacttcttt
     2821 atatctactg tctaaactaa cttctctatt gtctcccatc gcaaatactt ttcccttagg
     2881 aatcaccata tcaatatcgc cttcagtata attatcatgt atatatgaaa cttcatttaa
     2941 taacttacca tttatataaa ccttagaatc cttaattttt agatggtcac caggaacacc
     3001 tataaccctt tttacaagct cttttttcct tccattctct tgtaataaat cagtcttaaa
     3061 tatcattata tctccacatt ttggttctga taacttatat gtcatcctgt ttgctaccaa
     3121 ataatcatgt gatttaagtg ttgggtacat tgattctcca tttactaatg ttggttttat
     3181 aaaacgagtt ataataaatg caaaaaaaag tgcagtaata attattttta tccactctac
     3241 aatctcttct ttaatagttt cattcattca caaattctcc tttatagtta tattatcttt
     3301 attgataatg tctcttaaac actatctttt agataagtct actaaataag gtgttgtctt
     3361 ttttatatag acaactattg tgtatggtct ggtgatttca gctaatcagc gattgctcca
     3421 aattttatat gtttagtcaa tagttcctat cttagaaaat ggatacaatc tgactagaac
     3481 ctttcctaaa atggtatgct cgtcgaccaa tccaacctca tcgtatctac tatctaaact
     3541 tttttctcta ttatctccca ttgcaaataa ttttccttct ggaactacga tatcaatatc
     3601 tccatcagta cgattattat gtatatagga agtctcatct aacaacttat catttacata
     3661 taccttggag tcttgtattt ttacatggtc accaggaacc ccgataactc tttttactaa
     3721 atcttttttc tttccatttt cctctgttaa gtcggttttg aatactatta tatctttgta
     3781 ttttggctct cctaccttgt acgcaattct gttaactatc aaataatcac gttcaactaa
     3841 tgtaggatac attgattctc ctttgactat tgttggcact ataaaacgag ttattgcaaa
     3901 tgccaaaaca agagctatga caatcacttt tatccattct acaacttctt ttttaactgc
     3961 ttcacccact aaaatcgtct cctctgtgtt aaatattaag tcaatctaca gcaaaataat
     4021 ctaccataaa cctacaatat aaatattata cttctttttt ctaatagttt aaaatacttt
     4081 tctaagacct ttaggataat gattcttaat tacaccagaa gattcttttc cccaacctaa
     4141 aggtattcct tcaaccgaaa ccaaaaccca tcctctacta tcattagaat ttacaacatc
     4201 cccttttaaa tatttaagaa ttgattcact ctgtacactg aagttttcaa cattttttac
     4261 atcttcaact tttaagtaat gagatagtgc atgagaaggc tcaaatctat tcttctttaa
     4321 aacccctaaa tgcaaaccat atctcaatac ttttagtttt ttcgtatctg gatatatttc
     4381 aggaagtagg tacaaatttt ctcctcttaa ataaaattta ttttttttat tactataaaa
     4441 atcaaccttt aaaaatttct tttcaaactc cctataatcc tttatttctt tatctaaatt
     4501 ttttattttt aattctttga cattacattc ttcttcttct aattttttta ttttggcaac
     4561 aaaatgacct tctcccttta tcttatgagg ccataatctt tccatttcaa taagttctgc
     4621 atgtggatat tcactgataa actcatttat aacatgctca ttttcctccc ttgaaaaagt
     4681 acaagttgag taaattaaaa tgccatcctt ctttaacatt ttatatccat ctcttataat
     4741 ctctttttgg atggattgac attctactac ctttgcataa ctccaatctg atatggcaac
     4801 ctcatccttc ctaaacatac cttgcccaga acaaggtgca tctataataa ccttatcaaa
     4861 atacccatta aacgtctttc ttaagctatt agaatcggtg tttgttataa tacaatttct
     4921 cgctccaaat cgctctaaat tttcccctag agccttaatt ctactagcat taatttcatt
     4981 agaaaccaat aaacctgtat cgtttagctt agatagtata tatgtagatt ttccaccagg
     5041 agcagcacac atatcgagaa ctttttcacc ctctttaata tctgcctttg gtattacact
     5101 cattgcagaa ggctcttgca aatagtatgc tccagcctca tgcagtggat ttctaccagg
     5161 tctgtcaatt ttatcatcat aataaaaacc ttcttctgcc catggtattt gattcaaatc
     5221 aaacaactcc aaatcaaata attttttctt gtcaattttt aaagtattta ttctaagccc
     5281 agtagtctta ctttcctcat aactttttat aaaatcatca tattcatctt ttagtatttc
     5341 ttcaatatct tctaaaaatt cctttggtaa ttctgtcaaa tctataatcc cccttcaatc
     5401 tgctttactt gtgtgtcatt tattatactt taattattta tgtctttcct ttaaatttta
     5461 tttcttcttc tttttcctaa gattaccgaa aaacactttt ttagagtgat tcatatattt
     5521 ctcaagttta tcaagattta tcttccacaa aattattaca taaactataa gtaatataat
     5581 agcccatcta tattctatca tcaatatgaa atcaaaaact ccatgtaata aaattggaat
     5641 taacaccgcc ataaacaaat actcacgtct tttattttta tttccctcaa atttatactt
     5701 agaaatatag tagcccatag taattgcaaa cattacatga gctggtatag agataactgc
     5761 cctattaata ccaacttgca gtgctagatt tctactttca gaaaaaatat aaaccatatt
     5821 ttcaattgtt gcaaatccta aagctaaaaa tacagagtaa ataataccat ctaacttttc
     5881 agtaaagtgt ttttctttaa ttagggcagg aatcagtatc agagctttta caccttcttc
     5941 tacaagtcct gcaactacaa atgatacata tattagttct gaataccctt caaatacatt
     6001 aaacttcatc agtaaatttt ctaaaagtat aattataaca cttagaaaag cacctattaa
     6061 aaaaaattta attagatgga ttaggggttc tctacaatat ctatctttag accttatcca
     6121 aaacatccct atcaatatag gaattattgc taataaaaat aaatctagct tcatagatta
     6181 ttttcctttc aaaatttttg atttatttat gatttatatc atctacttat ttttaactta
     6241 ttcgtgtttt aaatacatac taaaatatac attaaattat tttaaattat ttaaatacaa
     6301 atatactcaa atactcaaaa aaatttaaat ttatatctat attatttaac aaatttcaag
     6361 ctatattcat atcttgaaat ataagggaac taaattcatt taaagaaagg gtgatttgat
     6421 gcaagatggt ttcttaacag taagcataat tgatgctact aacaatagac ctattcaaaa
     6481 tgcagtagta aacatatatt caatgtctaa tggtagccaa agctcatcaa ctttatacca
     6541 aaacctaaga agtaatgaat ctggtcaagt gactggttta gtattaccag caccagatgt
     6601 agattactct ctacaaccat ctgatgttag accttatagc cagtatatag tggaagccat
     6661 agcagatgga tatgagacag ttgtcataga aggaacacag ttgttagcta caattgaagc
     6721 aagacaagga gttcccatgt ctcctagaac aagaagtaag cgttctttct caagacaaag
     6781 tgagttgatt tttgatatag gagaacatac tctatatggt acttatcctc ctaagattcc
     6841 agaaagtaac ttaaaaccat taccacctcc tactggtttc gttgtattag ataaccccgt
     6901 agtgcctgaa tttatagtag ttcatgatgg tttgccagaa gattcatcag ctccaaacta
     6961 ttggattcca tttaaagagt acattaaaaa catagcgtca tctgaaatat attcaacttg
     7021 gcctgaacag acaatctatg caaatgtaat agctataata tcatttactt taaatagagt
     7081 ctttactgaa tggtatagaa ataaaggata taactttaca ataacttcaa caacagcata
     7141 cgaccataag tttataaata atagaaactt atttgaacca attaacgtag ttgtagatgc
     7201 tatttttaat acttttataa aaagacctcc tacatcaaga cagccactat tagcacaata
     7261 ttgtgatgga caaaaatccc aatgtcctga ccaaatgact caatggggca gtaaagactt
     7321 aggtgaccaa ggatatgact atgagagtat actaagatat ttctatggag atgagatagt
     7381 atttgaaaga gcacctatag taagtggtgt accagtgtca ttccctggta ctacactaca
     7441 agttggttct tctgggcagt atgttagaac catacaaaat cagttaaatg ctatatctaa
     7501 ttcttatcca gctgtaccaa aagttataga agatggtatc tatggaacag atacagaaaa
     7561 cgctgttaag atattccaag gaatctttgg tttacctcaa agcggagtag tagattttaa
     7621 aacatggtat gaaatatcca gagtttatgt agctacaact agaatagctt ctttaaatcc
     7681 tttaatttaa agcttgattt agaacataaa ctcaaattat tgaataaaat tattaattta
     7741 aaataaaagt ggctgttatg aactaatata taccataagt attacttata agtattgtag
     7801 tcttaacagc cattttttag ttataacttg tttttttaga atcttaaaac tttaaatggg
     7861 ggattttaag cttcttaaaa acagtgctta gcaacttaga tttctttaaa atattatatt
     7921 tatttttaag tttaataaaa tgctattcta acgaaaaacc tactttttat ttaaaatctc
     7981 acttattatt taaaacacta tccatcactt agaatattac ttcttatata aattattatt
     8041 tattataaat acaaataata ttaatttaaa aagttattcc taatttaaat aatactacta
     8101 tcttcatcag tgaacctaaa ctgcaacttt agacttatta gctaaagcca atattagacc
     8161 taaagctata gatgcaaatg cagaagcaat tactctatac tcagcaggga ataaaaatac
     8221 aattgtatga catggtatcc agaaaaatgg tatagttttt aaaacactaa aaccaaggta
     8281 agcattccag tccacaatat ttaccacatc tttaagtttt accttaatcc cctttccaac
     8341 ctcatatttt ttatctataa aacagtccat aaccttatgg aataaaaata cagttggtga
     8401 cattagtata ttcatagttg cactcttaaa tagagcattt gcaaaagcta ttccctcaaa
     8461 tggaagcata ccatccactt gtgcttgacc tacacctgat gagtagattg acattccata
     8521 tgtggtaact acacctatta ttccccaaac tattgctcta taaaatactc ctactggtat
     8581 tttccaatcc ttatacaaaa ttctaacacc aagtaaatct cccattgtag ctaatacagc
     8641 aaactttaaa aaacctccaa tatatggatg tgcacctgtt gcttccataa atacttctct
     8701 actacttggt attgctaaga cagcaataat acaaataaga actccactcc atataaaatc
     8761 gccttttttc atatttataa atactattta atatataaat agttctcctt tctattaaag
     8821 atttatttaa tttacaatct atctacaatt atttattaat cacttattat tagatattca
     8881 ttttccatat ttaaatttat tctatccctt tatctattta ttttcttact actctatcca
     8941 ccattatcta tatatttatt ttacgattac tctttcaccc taacccttta actatctatt
     9001 ttttcttact attcttatcc acatcatcca tatatttatt ttacgattac tctttcactc
     9061 taacccttta actatctatt ttttctgata ttcttatcca ccatcatcca tatatttatt
     9121 ttacgattac tctttcaccc taacccttta actatctatt tttttctggt attcttatct
     9181 actattatct gtatatttat tttatgatta ctcttttaac ctacccccta tactttatag
     9241 taatatgttt ttaacctttt tcattattcc caatatgctt tataactaca aatactctcc
     9301 ccccttaaaa ataataataa aattaatcaa taaatttata atcatattat tactctatgt
     9361 attaagcaat tactatgcca ataaactttg atttttttaa acaaatctta aaaatcctaa
     9421 aatgctaatt ttctttaact tttaatgaat ctaatagata acatttaatt ttcgttatat
     9481 ttttcagaat atttctatta ttatttttaa gaaaaaaaaa taatgttaac atttcttaac
     9541 atacattaaa aaatgttaac attataaatt gggtttaata cttttacttt gactcgattt
     9601 acttttattc tacttcttac tttattttta acttagactt tattttttta ttacttattt
     9661 gagtcttatt tattttattc tcactccata tgttcaatta catttgtcta aattctttga
     9721 tacttttatt acataatttt aacaagctta agtgttattt gggtagtgca gttgataatt
     9781 atttaaataa tcatcaactg catgtttata tcattacagt agcttaatgt atttaatcat
     9841 acttacttag gattttgaaa tatcttagag attatattta acattagtca attgctgcat
     9901 aattgtaagc aattgaaatt tgagacccat cttcattttc tatagtgata ggcttcataa
     9961 tttttaatcc ttcagttttt atcttttcta attcttgttc cattttctta attgtttcct
    10021 caaggtcttt ttgacttaca tctttgactg cattagggtc tttagcatag tcttttaatt
    10081 cttgaatatg tttagcatac tcttcataag tataatgctc aacaccatac ttttccaatt
    10141 cagtagggtc agttattcct gattttatta gagattcaaa tttaggtaaa gcaatattag
    10201 atgaatcatt tccatttgca aatactgctc cagttcctcc tagtaccatt gcagatacca
    10261 tcattgcact tataagggat ttttttattt tcataaccaa tcttcctttc ttagagattt
    10321 atttgggtag tgtagtttaa aatgactatt gcaatagtta tcaactacac tcttagacta
    10381 tcacagtagt catgtttatg tcaagccttt ttaaaaaact tttcaataaa aaatactatc
    10441 tcaaagcaaa attaattatt ttgagatagt attttaattc taaatctttt atattactaa
    10501 gatttatggt tattctatcc atgttctata ttgttcttta ttaactgcta acaaattccc
    10561 ttttcttaat atgacaaagc gattatcatt atagccactt cgctcaattg aaacttctcc
    10621 tgaatcagta taaagtacat ccttactatt actagattta tttacatacg tttcttcact
    10681 attctcagaa ttagttactt tgctcataga tgattcttca tgctctctaa attcaattac
    10741 agatactttt gccgtactaa aatgagtctc tccatcactt ttagctttac taacaagaat
    10801 atcagattct actttgggag aatataaatc agttgattta ctacttattg aaaatccaat
    10861 tttatcactt tgttcatttt ttgttaaacc aaaagattca acctcattaa tctctttatt
    10921 ttctgttgca aaaacaagac ctgtcccaca aactaataca gttgaaacta aaaaagtaac
    10981 ataagttgat tttttaaatt tcataatatt aactattctc ttttctattt ttgtctttcc
    11041 aaatccacta catattgtca ttgaaaatgg attattctcc tctgctagac ttattagtgt
    11101 ggttgcataa tcagccctat attcttcacc aagtatactc ataacctttt catcacaact
    11161 aaattctatg tctttattta gtaaaaaaaa catcacccaa actaagggat taaaccaatg
    11221 gacaacaaga gctacaagca taaataactt taacagatta tcaaagctat taatatgaac
    11281 catttcatga gttaaaacaa agtctaaagt ttgattatta gcataattca tttgctttgg
    11341 caaaataatt cgtggtttta aaataccaga tgtaagtggt gtcctagtaa attcagatat
    11401 gcaaacaata aatcttctaa aacggctatt cttacacatc cattttttaa tgaactcatg
    11461 attttttata tgaattgaag ttttaaaaag attaattatt ttaaaatatc taaagcataa
    11521 aagtatgcta aacataatag taactataaa agacaaaatg cccaacattt ccaacatcat
    11581 aatattttca atattagaca ttatttcaaa ttcgtcagaa ttgtttttac ctgtagagtc
    11641 actagctttc ttaagagtat aattttctgt tgatgtatta cccaattcca catcaaaagt
    11701 cgattcaatt atactataaa tactaaaacc agaatttaaa cttactggta ttagaagttt
    11761 aaataaaaca atcaaccaca ataatacaaa taccttttta ggtaattttt taaataaaca
    11821 tgctcttata aaaataacta ttaaaagtaa tactccagaa gtaaaactca tttctaacaa
    11881 tttaatgaac ataaatctta cctcacttta atttttcaac aatttgttta agttttgtaa
    11941 tctcatcact tgaaagattt tctttattaa taaaagctga aagaaatttt tctgaagacc
    12001 ctccaaacat tttattaatt aaagtttctg tttcctgttt ttgtacttcc tctttgctta
    12061 ttaaagcttt acaaacaaaa ttcggctcaa ttctctctat tgcacctttt gacactaatt
    12121 tttttataac ggtatacgta gtgtttctat tccatcctgt ttcttctttt aaaattttta
    12181 ctaactctcc agccttgctt tctcctctct cccatagaac ttccattact ttaagttctg
    12241 aatcaaataa ttttatctcc atataaaatc gctcctcaaa tatagttatc acaatagtca
    12301 ttactataat aaaaaactat catagtagtc tatactatgt caatcattta ttttttaata
    12361 ataaaaactg tctttttaaa aaattcttat aaaagacagt tttatcaatg ttatacttta
    12421 atgtttaatt aaagccatat tttatttaaa tatataacta tctatcttag tacatagtat
    12481 aagatttttc tttatctaat agtatatcct ctacatctgg gttttctttt actaattgtc
    12541 tataaaaatt acaattagta acacccacat atggaattag ccatagtaga cctattccaa
    12601 gagtagctat tgaaagtata taccatccta taaagctaaa gtttagtaca aataatttaa
    12661 gtacatttcc tttcatcata tctaaactag cacccattga tttaaataca cccatattat
    12721 ccttgtcttc acaaattaag aaagtagctt gagaaacata taatagcaat atataaactg
    12781 gtaacataag aacaagtatt ataagaacta gagttattcc agtagaaata atagtcccta
    12841 ttccaacatt atcacttgat aaatattcaa ttgctccact agtaagtaaa actccaaatg
    12901 ataaaaacgc tcctataatt gtgattataa aagaataaat atacacaata attgattcaa
    12961 ataaataaaa accaaaactt cttaaataaa ttctcccact tacaaagata tctcctatct
    13021 tgactttttc atctttagta attttcaaga acatacatgt aaatcctgct gaaacgcaaa
    13081 aagctattac agccactaca atagtagtca atggttcaaa aggtaatcct acaattgata
    13141 ttaatactat caatgcaata tatactgcca tagaaattgc acatatcccc cagtttccag
    13201 ataactgagc tttagatact cgttttaatt cttgccttga taccataaaa aacacccctt
    13261 tcattttata aaaaaacttt taaaaatata aataatcttt taacgtataa tattaatata
    13321 tc
//
LOCUS       DAGSWI010000113        13281 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.113, whole genome shotgun sequence.
ACCESSION   DAGSWI010000113 DAGSWI010000000
VERSION     DAGSWI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.113"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(288..3893)
                     /gene="cbpA"
                     /locus_tag="MJF80_002914"
     CDS             complement(288..3893)
                     /gene="cbpA"
                     /locus_tag="MJF80_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HBZ0281562.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAGGDANAGNAEGDANAGNAEGDANAGNAGGDANAGNAGGDANAGNAGGD
                     TNAGNPEGDANAGNAGGDANAGNAEEDANAGNAEGDANAGNAEEDSNAGNAEGDMNAL
                     VVEEATAEDPTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIV
                     IPKDVKAELIFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGM
                     RAGKGTTLIIDDSVVNRDIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRG
                     SLKVVGNYYSAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGD
                     ITINGGTITAKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSY
                     GGAHGSAFGDGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSL
                     KATTFKSLGGAVAYGDLEKKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKIYPYG
                     APSYTDDQGVLYLWFSEQFKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQFLKQ
                     YEEFTIDDTPPYLIIKRYDGLPFEEDVEKSFLDWILKDGGIPIITPKGEKLTDKKFMT
                     ISSQRLNEETMEPDTNLGSDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHRAYYK
                     AEIIPADTETSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYIN
                     GKEYKDPVELKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIE
                     GSEDEKKLKLDVTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGE
                     WKPSTEGGNGDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGS
                     GGYDTGNPNLNKPDPDNGGNSKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNG
                     GNSKPDPDNGGNGKPDPDNGGNNKPDPDNGGNSKPDPDNGGNSKPDIDIDIDTDGDGK
                     PNINIDTDGDGKPDINIDTDGDGKPDVNIDIDGDGKPDVNIDIDGDGKPDINIDTDGN
                     GKPDINIDTDESLDSNVGQNVQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
     gene            4162..4733
                     /locus_tag="MJF80_002915"
                     /pseudo
     CDS             4162..4733
                     /locus_tag="MJF80_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
     gene            complement(5745..6407)
                     /locus_tag="MJF80_002916"
     CDS             complement(5745..6407)
                     /locus_tag="MJF80_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281563.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(6424..8979)
                     /locus_tag="MJF80_002917"
     CDS             complement(6424..8979)
                     /locus_tag="MJF80_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBZ0281564.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPNDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKSDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLFRLMGAKKRNVRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEALYSEAGLNPKLYINNEIVIKAILVGIISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLIAVVVSNRDFESMNIIEGIKENE"
     gene            complement(9212..9406)
                     /locus_tag="MJF80_002918"
     CDS             complement(9212..9406)
                     /locus_tag="MJF80_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBZ0281565.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTKEN"
     gene            complement(9418..9597)
                     /locus_tag="MJF80_002919"
     CDS             complement(9418..9597)
                     /locus_tag="MJF80_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281566.1"
                     /translation="MIHVEISEKEFFRKIEEVFDKGEGNIEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(9602..9859)
                     /locus_tag="MJF80_002920"
     CDS             complement(9602..9859)
                     /locus_tag="MJF80_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281567.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLENLEYTKEQSSQVLH
                     YSMTKDKKGTKEILSIQCVKDFSYNEKLSEGDKFIMAIYNI"
     gene            complement(9832..9999)
                     /locus_tag="MJF80_002921"
     CDS             complement(9832..9999)
                     /locus_tag="MJF80_002921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281568.1"
                     /translation="MKIKKITITMVIAISLTLGLITGCTNKVSDTNEKIYTIEQISSI
                     KKTCQRKYQNQ"
     gene            complement(10120..11513)
                     /locus_tag="MJF80_002922"
                     /pseudo
     CDS             complement(10120..11513)
                     /locus_tag="MJF80_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            complement(11507..12182)
                     /locus_tag="MJF80_002923"
                     /pseudo
     CDS             complement(11507..12182)
                     /locus_tag="MJF80_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(12822..13226)
                     /locus_tag="MJF80_002924"
     CDS             complement(12822..13226)
                     /locus_tag="MJF80_002924"
                     /inference="COORDINATES: protein motif:HMM:NF019976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HBZ0281569.1"
                     /translation="MKKYTNKIIINAHKVYINNEKIITLSNEIDLIAVVTKKLFIIEC
                     KDIYYKFTPYGFKCDINKTYSFISKMEKKYKSVLENKQNLEIKFGCEIEEIQPIIVFK
                     TYNMAINSKLNNNRIKIINFNGLEKLIRDTKN"
BASE COUNT         4255 a         2209 c         1355 g         5462 t
ORIGIN      
        1 taaaactaaa atatttatta tcatcttcag caacaatata aatctactaa ttaaatacct
       61 aatatttatt catatttttc taaacataat tactaaaatt gaattaatga tatcaattcc
      121 attaatctct tttaataaaa aattctcttt tctatatgaa aattatttca aaactttaaa
      181 gttaaataca aaaatgacta taaataggtt tttatttaca caaatctatc tatagtcact
      241 atagtttatc caataaaata ataaaataaa tatgtacaca ataaatgtta tttacgtctt
      301 aagtatttat ttgttagatt tttaattaaa aaaaacaagc taatgaacat taatgctaaa
      361 tctagcatta tattgctttg atctcctgtt tgtacatttt gacctacatt actatctaaa
      421 ctttcatctg tatctatatt tatatctggt tttccattcc catctgtatc tatatttata
      481 tccggctttc catctccatc tatatctata tttacatccg gctttccatc tccatctata
      541 tctatattta catctggttt tccatctcca tctgtatcta tatttatatc tggttttcca
      601 tctccatctg tgtctatatt tatattcggt tttccatccc catctgtatc tatatctata
      661 tctatatctg gtttgctatt tcctccatta tctgggtctg gtttactatt tccaccgtta
      721 tctggatccg gtttgttatt tcctccatta tctggatctg gtttaccatt gccaccgtta
      781 tctggatctg gtttgctatt tcctccatta cctgaatccg gtttgctatt tcctccatta
      841 cctgaatctg gtttgctatt tcctccatta cctgaatctg gtttgctatt tcctccatta
      901 tctggatccg gcttgctatt tcctccatta tctggatctg gcttatttag atttggatta
      961 cctgtatcat aacctcctga gccagtatct acatttttat caggcttcca ataatcatct
     1021 actccatcat tatctgtatc tattgctgga cgattataat ctgtatcagg gtttccatct
     1081 ccattggtat ctatattttt atcaggtttc caaataccat ctccatttcc accttctgta
     1141 ctaggcttcc attcacctgt attatcagta tctatgttaa tatctggctt ccaatcacca
     1201 ctcgtatcta tattgatatc tggccttcca tcaccatcag tgtctatatt tatatccggt
     1261 attccatccc catctacatc tacatttgtc acatctaact ttaacttctt ttcatcctca
     1321 ctaccttcaa tgtaatttat atcatctcca atatatttta cagtaatagt ttgttcagta
     1381 gtaaaaatat gatgattgct atctaaaggt ctccattcaa tagatgcagt actataatta
     1441 tgactctcac tacccttatt atgagttttt aattctactg gatctttata ttcctttcca
     1501 ttaatataga attgtacgta acctcttgga gaggcacaat ctggtccttc tttcttatct
     1561 ggtgaaatag taacatttaa tgtaaacgta tctgttggtt taaaaacatc agttttagct
     1621 ttagactctt ctacaactaa tgaagtttct gtatcagcag gaataatttc tgccttataa
     1681 tatgctctat gcccccaata agcttcatta aaaggatata catttgaaaa ctgtgttgat
     1741 gtaatgctta attgatattt tccagcatca acttgctttg taaggtcatc acttcctaaa
     1801 tttgtatctg gttccattgt ttcctcattt aaacgttgag aactaatagt cataaatttt
     1861 ttatctgtga gtttttcacc tttaggagta attattggaa tacccccatc ttttaatatc
     1921 caatctaaaa atgatttttc tacatcttct tcaaatggta atccatcata tcttttaata
     1981 atcaaatatg gtggagtatc atcaatagta aattcttcat attgctttaa aaattgagaa
     2041 cctgaaccta tatctttaac ataaaaatca tctggtttta tatttcctcc agtaatttta
     2101 tctataacaa ctaaattaac agatacttca gatcccttaa attgttcaga aaaccataag
     2161 taaagaaccc cttgatcatc tgtataacta ggtgctccat aaggataaat ttttccatca
     2221 attagcattt taaaatcatc aactaatgta gtactaactt tatcttgtcc taaatacttt
     2281 aaagatattt tattcataaa aacttttgtt ttcttttcta aatcaccgta tgcaactgct
     2341 ccacctaaac ttttgaatgt tgttgctttt aatgaaccac ctgttactat aacatctccc
     2401 gttgtagcac ttaggtcaaa aatccaatct cccactcctg tcatataagg taaaatcgtt
     2461 cctccagtta caagtatacg atatcctcta tttgttcctg gattaacaca tccatcccca
     2521 aatgcactac catgtgcacc tccatatgaa taggaaagtc caccattaat tgtaatattc
     2581 ccactataac cagaacctgt gctaggatct actactgagg gacttaatgc attctttgtt
     2641 gttgtatagt atcctccacc tattccagct ccatggtatg cacctttagc agttatagta
     2701 ccaccattga ttgtgatatc tccaccacta ccattaccgc ctccacctat tccagctcca
     2761 tatcctgtat tacgactcga ttcttgacct atagcagtta tagcaccacc attaataata
     2821 atatttcctc cactttcagt atttccaccg ccaatacctg cagaataata atttccaaca
     2881 acttttaaac ttcctctatt attactatct agtagtgtaa ggcgatctcc tttttttaac
     2941 ttgtctccat tttctagagt tacatctcta ggaatttttc caccctcagg aactataaac
     3001 tcgccttcaa tatctctatt tacaacagaa tcatcaataa ttagagtagt accttttcct
     3061 gctctcatac ctggataata tacatcacct gactctagct tgttttcact accatcttgt
     3121 aatataacat gtgcatatgc accaccatct ttatctggtg ttaaagtaaa tgggctatta
     3181 gatgcagttt taatatttac tccatcaaat attaattcag cttttacatc tttaggtata
     3241 acaatcctat gtgttgttgg agtatttgga ttattatttt gaattgtcat ttgttttcca
     3301 ctaagaatag ttaaaacgcc acttgcaaat ttaaaatcga catttatttc tccaccacta
     3361 attttaaagt ctcctacaga tgtaggatct tctgcagtgg cttcttccac aactaatgca
     3421 ttcatatctc cttctgcatt tcctgcatta gaatcttctt ctgcatttcc tgcattagca
     3481 tctccttctg catttcctgc attagcatct tcttctgcat ttcctgcatt agcatctcct
     3541 cctgcatttc ctgcattagc atctccttct ggatttcctg cattagtatc tcctcctgca
     3601 tttcctgcat tagcatcccc tcctgcattt cctgcattag catcccctcc tgcatttcct
     3661 gcattagcat ccccttctgc atttcctgca ttagcatccc cttctgcatt tcctgcatta
     3721 gcatcccctc ctgcatttcc tgcattagta tctccttctg catttcctgc attagtactt
     3781 tcttctatag tatctgcgaa agtatttgac gcattaaatg atgctactga tccaagtccc
     3841 attgagatac ttaaaatcat tgatagtgct ttctttttac atttttgttg cataaattcc
     3901 ccctcccctt aacaattttt aatatacact gtatacatcc ttatatacac tctaataatt
     3961 cttaatatac actgtataca tccttatata cactctaata atttttaata tatacttaaa
     4021 acttatattt gtcaatgaat atacagtttt tttcaacctt atatattata agtattcatt
     4081 atttgctaaa taactacaat aaaaatttta attatataaa atttatttaa tcttttatat
     4141 aataatacaa ggggtaatta attgaaaaaa ctagtaaaaa tattaataaa tattatttgt
     4201 attattacgc taactttctc tagcctttcg atttacataa aattatctga atataaaaag
     4261 ctgatgaagt atatacggaa ctcagagaaa acactatcaa taatagcaaa catcaagaac
     4321 tttatgataa aaataatgat tatagattct ggcttaaaat aaacaacgta aatatagact
     4381 atcctgtagt acaaggatat aacaatgatt tctacctaac tcatgatttt tataaaaatt
     4441 atcttccttt aggtagtatt ttcatggatt atagaaataa ttttgaaaat gataagagtt
     4501 taatagtcta tggtcattat atgaaaaata aaactatgtt tggtcagcta gaaaactata
     4561 ctgatgaagt atttttcaaa gaaaataatt tagtagaaat aaattataaa gtacaaactt
     4621 atacttatga aattttttca gtatatactg ctgacttaat taacagagat tatttaagta
     4681 tacactttaa taataatgat gagtttaaat attctttgaa ttatataacc taaaaatcag
     4741 tacacaaatc tgatataaag gtagatattt cagataaaat tataactttg tatacttgta
     4801 aatatgaatt taaagatgct cttactatag ttcatgctaa attaatttca aaaaagagac
     4861 ctataagtag gtttgtgtaa atgcagactt atctacagtc ttttttgaac gtaaaattgg
     4921 taagtataga ataatattca tattaaactt ttgtaggcaa agaaatgaaa aatcaaagag
     4981 aaataattat tacagattta gattaccaag ctgaattaag agagtgtaaa actatggagg
     5041 atgtagttgg tgaagataat atataatagt ttaacttata aattatcttg gtgatattaa
     5101 tgaagtttca aagtatatat tatttattga tataaataaa attaaatctg tttctaatag
     5161 atgtaccttt gtttggagac aaactattga agaaatataa ctaaaatgtt gaagtagcta
     5221 cctgacttta ttttgaaatg cgaacaagat tttggcatta agataattta gaaagaatca
     5281 ctggtacaca aggatgtgaa cttagaatga atagaagtat tcaggcataa ggagctttca
     5341 cccaaataaa acatagtatg aactttaaaa gaattctaat caaaggcaat caaaatattc
     5401 tttcagagtt tatacctgta gtgttagcct atagtatttc taagtttcac aaataagata
     5461 atcattaata aatgtagtat gtatattcat cctctcaaaa atatttttta atcttacact
     5521 ttgtaaatta ttctatttta aaattgtatt tttatacctt tatttcttat gaccgttttc
     5581 aagctcaaat tttgcataaa tataaattaa tgaaaagcaa gtaaaaaaaa actgtcgcac
     5641 agcaatttaa aattactcat tcgacaatcc ttttttcgtg gatttaaata gtggagaaga
     5701 atcacaagaa taatacccac gattaaacct ttatacaata cattttatat gactttacca
     5761 tcactaatag ttattataca atcagccatc ttagctattt catcattatg agttatcata
     5821 accaacgtct gcttgtattc atttacacaa tactttagta aattcatcac ctcattagtt
     5881 gttttactgt ctaaatttcc agtaggttca tctgcaaaaa ttattgatgg cttattggca
     5941 agtgatcttg ctattgcaac tctttgttgt tgaccacctg ataattcatt aggaaattta
     6001 ttaatctgac tttctaatcc taacttagtt attaaatcat taatataatt attatctatc
     6061 tttcctttag caattaaaat aggaagaact atattatcat aaacattgat aacaggtatt
     6121 agattatagt tttggaatat aaacccaaat tctttacttc taagattaga tagcttttca
     6181 tcacttaaag tatataaatc taaattatcc ataaatacat tacctaaagt tggtttatca
     6241 agccctgcca tacagtgaag taatgtgctt tttccagaac cactttgtcc aattattgca
     6301 ctaaatttat ggggttctat ctctaaagta attccatcaa cagcctttat ttcattttct
     6361 ccttttccat atattttttt aatatctata acttttaaca tcttcataca tttacctccc
     6421 aaattattca ttctctttta taccttcaat aatattcata ctttcaaaat ctctattact
     6481 tacaactact gctattaaac atacaaatac agatatagca aagaatagta aagattcctt
     6541 aattgggaat gtaaaagaca taaaattctc tattccatag tttatatacg catcattatt
     6601 ccaatttgta aatttaacta gatttataaa tgatggaatc atagctatta taaagctaaa
     6661 aactgcatat gctaatgctt catatataag catttttttc atatttttaa gactaacacc
     6721 taatgctcta agcgttgaaa tttctttttt tctaattagt agatttgttc ttatagtaca
     6781 gaaaatatta attgatgcta ttattaaaac tagtgcaatt attgctattt gtgatttaat
     6841 gtaactttta tatatacttt ctgacatttc atgataactt aatctactat taaatagaga
     6901 ccctggtata cttcttgaaa ttttctgtat ctcattatta acaatctcat catatggatt
     6961 ttctaaattt atgcctaatt gagtgtattt ttgttctcct gttatgtttt tagagtgaat
     7021 attacttgtt ataatttcta gatctggcat aaaatcgcca tctcccatag caatccattc
     7081 atcctttaaa attacagcca ctcttacctt catctcttta taaacaattg tattattttc
     7141 ttcaacagga atttttacag ttattatgtc tcctatattt aattctttga atataggctt
     7201 tatagtatga tcatcaacta tatcataaaa ataattatat actgctacat ttggatatcc
     7261 atcagtgtcc tttttaatat catctaagct accttcttca acaaagtcat ttaacttttc
     7321 atctatataa tcatcatcat aaagctttaa aactaattta tcttccatta tctctttttt
     7381 ttctactcca ttataggttg taaattcttt gtttatatcc ttattattaa aagtaaacat
     7441 tccatttgtt atttgaattt tatgtatact tttaacaccc ttaatttttt ctatattctc
     7501 tacatcacta tccgtgtatt ttgatatttg cgggtctgca tttactccaa attgcattgt
     7561 tatattatat ctattttgca tatcttcaat tttactgttg acctcttctt gcatacttga
     7621 gaatgtagta atgaatatat atcctcctaa agcaatagac aacatagata tagcagctct
     7681 aaccttattt cttccaatat ttttaagtcc cataaatcca taaaatccaa atattttgct
     7741 tactagtctg tctattccaa gtctacttct gtgattttta attttgtctg tatttattat
     7801 tgattcaata ggtgatatct tgccgatttt aaaagttggt ataatacaag atataaaaac
     7861 agaaattatt ccaaccaata tagcttttat aactatttca ttatttatat ataatttagg
     7921 gttaagacca gcttcactgt ataaagcttc gttataatta ctaattccta tatatgaata
     7981 tgtaacccca gctactaacc caagtagtat acctattatt gttataatta atgcttgata
     8041 gattatcata caccttacgt ttcttttctt tgcacccatc agtctaaaca gtcccatttc
     8101 ttttactgat tgatttagtg taatattgaa aatattaaat atgaaaatta tagatactgt
     8161 agttattaaa acatttcttt taaaaacatt cggatcactc ttatcatctt ctacataacg
     8221 aagttccctt acaagaggta catttggtag aaagcttttt tgtcctaact tatatttttt
     8281 cataagataa tcacattcac cttcaatatt tctccatcca cttttaaaac ttaatatact
     8341 attataacta atcgcatcat taggtataat gttgtttgtt tcatcattgc cataagttac
     8401 tgcttcgtat tgccaaacca catcatagta cccttttggt ttttccataa ctcctactat
     8461 tttaaattct ttatttatag aaaataattg attacttccg ttagtatctt tatagttctt
     8521 ttttattgtt aaatttaatg tagatcctac tccttcactt aaattcattg cttctagtgc
     8581 ttttttctct aatactattt cattgttgtt tttaggtagt ctaccttcta ttattttgtt
     8641 gtatctactt tttagatact cttcgtttga tgaagttaat ataaatgagt ttcctttttt
     8701 atctgaaata cgtcctagat tttgtaccga attacccttt gatacttttt catcagtttc
     8761 taatttttta gcatcatcaa gtgtcaaatc actaaactct acatggtatc cactgtaaag
     8821 ttcatgtatt gctactacat tgttttcatt ttgtgaatct tttataacac taggtccaaa
     8881 tatcaacata acacctaaca taactcctat tattaaaagt aatgttctaa gtttattttt
     8941 ctttaaatat gctagtgaca tttttaagta aatccccata taatcctcct attttactat
     9001 aaattattta tccaactcat ctgttaagta gattggtgga tattttaaat cttttaattc
     9061 ttcattattt tttgaagctt actaccatct tcctcatgat aaacttgtag tatcacctta
     9121 tttacatgta gtgcataata atcaccacta taaaaatatt ggtattcata acctgaataa
     9181 tttttgtaaa atgatttata aagtacccat cctaattctc ttttgtatac ttttcttttg
     9241 taatacgctc tatatcatca cctgaaactc cttgatatgt catatattta tctatatatt
     9301 tttcagcttc attccatttt ctaaaatagt tgttgtatat aataaatcct attatggcta
     9361 taatgcttat aattaaagtt aataaaatag ctttgtattt tctcatagtg aatcttccta
     9421 cttcaattta aattctttaa taagttttaa ttctaaagac ttagttttat catcaaattt
     9481 cttaacttga atttttatat ctcccaaatt atcaacctct atatttcctt cacctttatc
     9541 aaatacttct tcaatttttc taaaaaattc tttctcactt atttctacat gaatcaatga
     9601 tctatatgtt ataaatggcc attatgaatt tatcaccttc tgacagcttt tcattataag
     9661 aaaaatcttt aacacattga attgataata tttccttcgt acccttcttg tccttagtca
     9721 ttgaataatg taaaacttgg ctagattgtt cttttgtata ctccaagttt tccaaagaca
     9781 aaacaaaact tccatctgat ttattttcag ctaccctaag accactattt tctattgatt
     9841 ctgatatttt ctttgacaag tcttcttgat actagaaatc tgttcaatgg tatatatttt
     9901 ttcatttgta tctgaaacct tatttgtgca tcctgttatt aaacctagcg ttaagcttat
     9961 agctataacc atagttattg taattttttt tatcttcata taatccactt tatatattga
    10021 aaactcgtta tttatcataa aaaaaagacg ctatcttttt ctattaaaag ataacatccc
    10081 tatatgttta gtatcaatat atgttcccca actggtaggt taatcgaccc aactatcagt
    10141 attttgattt aatggtatat aaatattcaa tataaatttg tcttttttat cttcaaatga
    10201 taaatctcct ctatattttt ttatagaaga tttgatgctt taaatcccta ttccatgtac
    10261 aagtttatct tttttatctg ttaataataa acttttattc tttctaattg aatttatttt
    10321 actattctca catttaatta cgaaatagga ctttgagata gttcctttta tttgtatata
    10381 tttatcaata cttttatcat gtatcttatc gcaagcttct attgcattat ctaagtattt
    10441 gcaaatatat tagatacatc tattggtttt ataaaattca ctttggaaaa attgatatca
    10501 caaatgaatt taatttttct tttttcactt acatcacttt ttttatttat tattatatcc
    10561 aatataacat tacctgtatt gtaaattgat ttaaattctt taatttaatc ttttaaacta
    10621 tttacatacc catcaatttt ccttttttat atttgagatt ttctatacaa tatatatgat
    10681 tatttatatc atgataaagc ttcttaactt tagcgtgtga ttcttgaata cttagataat
    10741 gagaatactg catatctagc ttatttttta ttacttcatt ttgcgatttt aacttatttt
    10801 ccttaattgt ttttataaac actataataa ttactaagct actagctagt attaacctag
    10861 gtatcacaat atacccaact atatatattt cctaataccc accaaatcga tacatttttc
    10921 tatttatata aattattaat atagtaaata aatttgttat taaagcaaca gataagtata
    10981 tatagtttat tttattatct ttatctagtc ccaatctttt attaataaat ttaaatatag
    11041 cacaagctac tatataacta ttattataag ataatagtat atatctactt tagcattatc
    11101 ataactaaac actaatagag cttcctctga tgatattaca ttacttgtaa ctacaaaaaa
    11161 taaatttata gatataacat cttttattaa agggtgatat acgtcagata agaaaaatga
    11221 aactatatat ttaacaattc ctatcttata aaaataatta tagtaagttc ccaagtaata
    11281 aatatcaaat aatagattat atcatataga tttttcttca tattataatc atgggtctta
    11341 tcaacgttaa ataatataat gattttagtt ataagataag ctaataatac agtaaaaaaa
    11401 gatattttct tagtcttatc tgtatcttta tcgtaagtta atatactttt tagtatcatt
    11461 attggtgtaa acaatgataa tattaataat attttattta tatggtttaa catattctat
    11521 caccaataaa ttttagaaac ccctctttta tatctttata tctatatcta ctaataggta
    11581 cttcttcttg actgtctaat attgcagtat ttgactttat attttctaca tagtttaagt
    11641 taattatata acttttatga catcttacaa gttcctctat atttaaatct ttttctattt
    11701 tttctagtat acatatagca tcaaaagttt tattttttgt atgtattagc atgttctttt
    11761 tttgaacttc tatatatttt tttgctagat tagattttgt atgtgttaga cttattttta
    11821 tttttttcaa tttttttgat acatgttaat atgtgttttt ctagttcttg aaaatttatt
    11881 ggctttaata agtatcaata agctcttact tcatatcttt cctgtacata atttattaga
    11941 gatgttataa atattattat ctacttttct tatgtttctt gcgatatcta tactatttag
    12001 ttctgccatt tttatatcta agagtagtgt atcctctcaa ttaactttgt aatggttctt
    12061 cttttgagct atattttttt aattcgatat tctttgtttt tattttttaa tatattatca
    12121 acttgatttt ttattacgat ttgtatttct ttttcatcat cacaagctgc tattgtaatg
    12181 atgacctctc ctagtattgt atttttattt agattattca ctttaggtta acacttttta
    12241 aatgtattta tcaataatat ttaaattaaa aatgtataaa aatacctata tccaattgga
    12301 tataggtatt cgtggatttc aatggtggat acgaggggag tctataagtg gttatttgat
    12361 tgttttgttg gttttgtaaa agttgataat acagcagttt acatataata ctatataaaa
    12421 tttttataat ttttttgtta tttgttttta taattggggt atagttgggg tataattata
    12481 taaagttatt acacactata atattaaact attttacacc ttttgatagt gatattacat
    12541 agtcattatg tatttacctg taaaaaatat tttattgtta tcaattggac atatgttatt
    12601 ctcatcttct agtactttca tagtttcttt atacatcaat tttaccttta taaaaacttc
    12661 ttctgttaag aaacaatttt aagttcaaac agaatcaaac atatcataaa ccttgataat
    12721 gctaaattac aaacaatcaa tataagaaat attcacttat aaaattatca tttttacttt
    12781 aattaataat taaaccataa aagtatatta ataatatcat gctaattttt tgtatctctt
    12841 ataagttttt caagtccatt aaaattaatt atctttattc tattattatt taacttagag
    12901 tttatggcca tattatatgt tttaaatact ataattggct gaatctcttc aatttcacaa
    12961 ccaaatttaa tttcaagatt ttgtttattc tctaatacag atttatattt tttctccatt
    13021 ttacttataa atgaatatgt tttgtttata tcacatttaa atccatatgg tgtaaattta
    13081 taatatatgt ccttacattc tataataaat aatttttttg taactactgc aattaagtca
    13141 atctcattag ataatgttat aatcttttca ttgttaatat aaactttatg agcatttatt
    13201 ataattttat ttgtatattt cttcaatata ttgctacaat ctataactag attatttttt
    13261 atttctcttt ctataatttt t
//
LOCUS       DAGSWI010000114        13129 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.114, whole genome shotgun sequence.
ACCESSION   DAGSWI010000114 DAGSWI010000000
VERSION     DAGSWI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.114"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            205..1086
                     /locus_tag="MJF80_002925"
     CDS             205..1086
                     /locus_tag="MJF80_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBZ0281570.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1294..2157)
                     /locus_tag="MJF80_002926"
     CDS             complement(1294..2157)
                     /locus_tag="MJF80_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBZ0281571.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2312..4246)
                     /locus_tag="MJF80_002927"
     CDS             complement(2312..4246)
                     /locus_tag="MJF80_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBZ0281572.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4358..5371)
                     /gene="galE"
                     /locus_tag="MJF80_002928"
     CDS             complement(4358..5371)
                     /gene="galE"
                     /locus_tag="MJF80_002928"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBZ0281573.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5395..6264)
                     /gene="galU"
                     /locus_tag="MJF80_002929"
     CDS             complement(5395..6264)
                     /gene="galU"
                     /locus_tag="MJF80_002929"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBZ0281574.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6462..6782)
                     /locus_tag="MJF80_002930"
     CDS             complement(6462..6782)
                     /locus_tag="MJF80_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281575.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6772..8763)
                     /locus_tag="MJF80_002931"
     CDS             complement(6772..8763)
                     /locus_tag="MJF80_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281576.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8886..11429)
                     /locus_tag="MJF80_002932"
     CDS             complement(8886..11429)
                     /locus_tag="MJF80_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBZ0281577.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11707..12041)
                     /gene="rnpB"
                     /locus_tag="MJF80_002933"
     ncRNA           complement(11707..12041)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="MJF80_002933"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12173..12850
                     /gene="srtB"
                     /locus_tag="MJF80_002934"
     CDS             12173..12850
                     /gene="srtB"
                     /locus_tag="MJF80_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBZ0281578.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(13010..13084)
                     /locus_tag="MJF80_002935"
     tRNA            complement(13010..13084)
                     /locus_tag="MJF80_002935"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13050..13052),aa:Thr,seq:tgt)
BASE COUNT         4046 a         2297 c         1339 g         5447 t
ORIGIN      
        1 gattttattt ataagataat tcttgtattt ttctattagg tcgtataaat atatttatct
       61 tataattcag acaaaaaacg ataaattttt ttaaaagctt ttattcttac tctataatca
      121 tatataataa aaatgtaaaa atatcttaaa ggttaacttc tacaaatata taaataaaaa
      181 tacctatgaa aggatgaaca aaaattgaat ttatatcact tacgctattt tgttacattg
      241 gcacatttag aacactatac aaaagcggca gaaaatttat ctataacaca accaagctta
      301 agtcatgcta tttctttact agaaaatgaa cttggggtgg ctctatttga aaaagaaggt
      361 agaaatatag tccttactaa gtatggaaaa atatttttaa aagatgtgga aaaatcatta
      421 gaaattttag attcaagtgt aaaatcatta aaaataactg gtactggtga aggacaaata
      481 gacttagctt ttcttagaac acttggtact gattttatcc cagacatagt gcacaaattt
      541 ttaaaatcta atcctgctaa atcaatagat tttaaatttc atactggtgt aactactgac
      601 attattcaag gtcttaaaga aagaaaatat gatattgcat tttgttcgaa acttgaaaaa
      661 gaaaagggaa ttgaatttat acctgttgcc aaacaagatt tagtactaat tgtaccatat
      721 agtcatccac tagctgcaaa ggatactata gatttaaaag aaaccatacc ttatcctcaa
      781 atagtattta atcagagaag tggtcttaga tatataattg atgatatgtt taaaaaaata
      841 aatcaacagc ctaatatagt ctatgaagta gaggaagacc aagtaattgc tggattagtt
      901 gcaaaaaatt tcggaattgc tgtagtccca aatatgaata tgcttagctt tacaaaagta
      961 aaagtaattc agattattca cccaagttgg gaaagaaact tttacttagc atttataaaa
     1021 gatagatatt tgcctcctgc aattaaaaac tttaaaaact ttgtcattaa aaacgctcaa
     1081 ttataattta ttaataaagg ggttattttt aaatatcctc ttttattctt tttataaaat
     1141 aaaaaaatct atacttttaa aagtatagat tcctaactga cagtcggcat ccgagtctca
     1201 gtaattatcg ttatacaaca gcaactggat tcagttcctg aataactagg ttacttacat
     1261 tatatcagat tttttaaaat ttgtcaattg tatctaacgc attttttcaa gccatggtcc
     1321 aacaggctct acaataggaa cttctttaaa tccccaattt cttatatcat tcacactcgt
     1381 tggagtatat attttgtttc tgacagattt gaatattttg tttttttctc catattgcat
     1441 acactttgga ttagcttcta ttaaccctct tcttgctctg ctaagaggat tacataaaat
     1501 atctgctatt tctaaacctt ctctatagtc agcatcttct tttttggcaa ttataaaacc
     1561 cttaacctta tctcttaatt cttcatctat atctaagtat attgttccat tattaaatac
     1621 atcaaaaaac gccttttgta cttttaagtt tgagacatca tcccttgact ctataactat
     1681 tcgtgcagat tctacagttt cattatttac aaatgaataa aaattctgaa gaacatgtat
     1741 aaaagccaca acatacggtt cctttgaagg tgcataatat ttctttaatc tagctttatc
     1801 tactgttact gatattatag tacactctaa tctagataga aataaaggca actcatcata
     1861 gaataactta atctgtgaat catcaaactt ctcaaatgct cttgattttc ttgatatatc
     1921 tgtcaaatgt attcctatgt tactggttcc aaaacaatac tctttaaatg catccatttc
     1981 tttcctcaaa gaacatacat aatcgccttg acttactgca tatttatatt caaaaattac
     2041 tcctgtcaat gtaaatgggt cttcatgatt tatatcagct cttccacttt catcaataaa
     2101 cattatatat tttttcttta taatcttact caccctctta acatatgatg atagcatcta
     2161 tattcatttt atatgagttt taaaaatatt ccaagatttt tatataactt aaacttaatt
     2221 ttatactaaa aaattgagat gtaaaacaat caagctttta ttgattgaat ttcacatctc
     2281 aattataatt atttttcaat atatattttt actattttgc aagaatatcc attaatttat
     2341 ccattactat ataagatact ccgcctccta tttgataagg agtttcaact tttttatttg
     2401 caaatactct ttgttgctct gatgataatc ctgaattatc cattaaaact attggtgaat
     2461 gtaattttac agctaatggt ccagctgata aagcatctac taaagcatct gatttactaa
     2521 ctaatacagc ctgtaaatca gtatctccat agaatttttc tatcactttg gcattagtat
     2581 ctactctact atctccatat attctatttc ctgaaatatc ttcagtagtt atatcattca
     2641 tttttgctat tactgaatcg ttaattgcac ttggtccacc tataaagtat gcattttcta
     2701 attttctttc tgttagccat ttataagatt cttctgtaat tccatcttta ttgactaata
     2761 ttataggttg tttttcttcc ccagctttag aagctacaga taaagcatct gcctccccat
     2821 ttccagaaac catatatgca gtttttattt cttctcgatt tccaagttct tcagctattc
     2881 ttgtagcagt ttgatatcta tcttgacctg ctattctctt tgtttctgtg ttgaaagtag
     2941 tttttatttc ttgctctaat ttagaactta ttgcattttc tccacctatc aagattactg
     3001 ttttaggtgc taataacttc atttcttcaa cagtttcagt tggtaaatta gctgtattag
     3061 taagtagtat tgttgagtca tagctacttg ctaaaggagt tgctgttata ccatcagcta
     3121 ttgcattacc atttactaat attacagtat ctgaactatc agcattccag tttccaactg
     3181 aacttatctt tttagctgta tcaaatctat ttgcacctat aattgtatcc catgtaggat
     3241 ttatagttcc atcttcatta aaagtataat ctttcccatc tattttttga gaaccagtta
     3301 gcatctttcc atctttgtct aaatagtaag acttaccatc tatttgttgc catcctgtct
     3361 gcattattcc tgttttgtca aagtaatact tattaccatc tatttcttgc catcctgtta
     3421 ctgctaaacc atcagatcct agatagtatt tctttccatt cttttcttgc catcctgttt
     3481 gcattactcc atctgagtta aagtaataag tttttccgtc tattactttc catccagata
     3541 ctcttgctcc atcagttcct aaataatatt ttttgttatt aattgttttc catcctgttt
     3601 gcattactcc tgaagcatca aagtaataca tttttccatt tacttttagc caactattct
     3661 tttggtatgt tccatcagat tttacataat acttcttacc attttctgtt ttccatcctg
     3721 tttgatttag atttatctta tattttgccg ctatctttac atagctgtcg ttactataat
     3781 ctataattac atctgaacct acaggaactt ggtcaaataa ccatctaaca tctttattgt
     3841 gcattcttat acatccacca gaaatatgct ttcctataga actctcatta ttatttccat
     3901 gtattccata tgtatctcca taagttcctt ttaatgctaa tcccatccat ctatctccta
     3961 atggatttct tggacttcct ccaggtatat tacctttata atatggtcta ttttttatct
     4021 tgttaactac ttttgttttc cctgttggtg tttctgaccc ctttttacct gttgcaactc
     4081 taaattcttt tacaagttta ttatttacaa aatatcctag agtatttttt cttgaattta
     4141 ctataatcac atgtttactg gctgcgcttg attctggtac gccacatgca aatactaaaa
     4201 ctaaagtcag tgctagtact aaatactttt ttagactttt tttcactata acttccccct
     4261 cttttagaaa cattactaca tgtgtctaat tataacataa aattaccaat accttatatt
     4321 tacaaatttg ttacatttat acatataagt atgtattcta ctcttcatac ccattaggat
     4381 tcatactttg ccatctccat gaatcttcac acatctcttc aagttcatac ttagctttcc
     4441 atccaagctc tttttctgcc tttgaagaat ctgcataaca catggctaca tctccagccc
     4501 ttcttcctac aatcttatat ggtatttctt ttccacttgc tttagaaaat gcttttacta
     4561 aatcaagaac actatacccc tttccagttc ctaaattata aacaacaagt cctggatttt
     4621 cttctaattt ttgtaatgct tttacatgac cagcagctaa atctaataca tgtatatagt
     4681 ctctaactcc agttccatca tgtgttggat agtcattacc ataaacactc aattccttca
     4741 atttacctac tgctattttt gttatataag gcattagatt gctcggaact ccatttggtt
     4801 cttcacctat tctaccactt ttatgagcac cgactggatt aaagtatctt aaaagagcta
     4861 tatccaatga cttatctgct tttgatatat ctactaacat ctgctctatc ataagtttag
     4921 ttcttccata tggattggtt acagaaagtg gaaaatcctc taatatagga caagtatgtg
     4981 ggtctccata tacagtagct gaagaactaa atacaaactt tttaacacca tattctctca
     5041 ttaattcaaa taaatttaag gtacttatta gattattgct gtaatattca attggttttt
     5101 ctactgattc accaactgct tttaaagctg caaagtgtat aattgattca atattatttt
     5161 ctttaaatac aacatgcatt tcatctttat ttctgatatc aatattataa aattttactg
     5221 gttttttaga taattccttg attctatcaa ccactattga gttgctatta cttaagttat
     5281 caactataac aacttcataa cctgattcta aaagttctat tgctgtatgg cttcctatat
     5341 atcctgctcc tccagctact aaaactgcca ttgtaattac ctcttttcta atatttattt
     5401 ttcacttact ttttttctca tatagtccat taaatcatct tttaaatttt ctctctttag
     5461 agcaaagtct attgttgcct ctaaaaaacc aattttatct cccacatcat atcttctacc
     5521 ttcaaagtta taagcataaa tagcctcttg ttttccaaga gtttgaagtg catctgtcag
     5581 ttgtatctct ccacctttac caggtgtctg tttctctaat atgctaaata ttgcaggagt
     5641 tattatatat cttccaagta tagctatatc agatggagct tcttcaattg caggtttttc
     5701 aaccatatct ttaactttat atactctatc ttcgatatgt tttacatcca atataccata
     5761 tttattagta tcttctttag ctatcttttg aacacctaat acagttgttt tatactcatt
     5821 ataagtatct attaattgtt ttaagcacgg aacatctgaa tctactatat catcacctaa
     5881 taatacggca aaaggttcat cacctataaa actttttgca cagtaaattg catgtccaag
     5941 accttttggc tctttttgtc ttatataatg aatatttacc atatttgata tgtctcttac
     6001 catttctaac atctctgtct tacctttttg ctctagttct aactctaatt caactgattt
     6061 atcaaaatga tcttctatag attttttatt tctacctgtt attataagaa tttcttcaat
     6121 tcccgattct atagcttctt ctattatata ctgtaaagtt ggtttatcta ctataggaag
     6181 catttctttg ggctgtgatt ttgttgctgg taaaaatcta gttcctagcc ctgctgctgg
     6241 tattattgct tttttaactg tcatctgcat tttttcaccc ttcttcgtaa attttctatt
     6301 gtaccaaatt aatataccat atttatactt tttcttcaaa ctctatatta tcccaatatt
     6361 taatgctata agataatata aggtagatga aatttcatct acctttagtt ttataagtta
     6421 atattgtatt gtttatattt tatccaaaac ttatacaggt actaaatttc atctaagatg
     6481 ccttttatat cactatctat atcttcatct agataagaat ctaaatcttc taagtcaact
     6541 tctagttctt gatatgtcat atcaacactg acatcctcat ctatatccat ttctgatatt
     6601 aattctccta aaatactatt tccatcagaa tctaaaccac tatctatatc ttcatctata
     6661 tagttttcat ttaaaatttc atctaaatca atctcgtaat aaacttcatt atttttttca
     6721 tcccctgagt ccaatcctaa gaactcatag ttaaaagtcc aaacattgta tttacttgac
     6781 atactttatt cctcctgtga aaaacatatt ttcatctagt ttattggttc cataatcaac
     6841 agagttatat attttttcaa ttatgtcttt tcttctttct tttgtactat tatttaatgt
     6901 caattgttta aatacatatg tatttgttat tctctcgaat aatatttctt ttatactatc
     6961 atcatcaata tttctatagt ttttccacaa taactcaaat aacttaaaca tttccctact
     7021 agtttcatct acataataag gatacatatt ttctactaaa tggtttaatc ttgatatggc
     7081 ttgttttaaa aatacatcaa gacttgcact ttcactttct tttaacattc tataattgta
     7141 tttcttctta tcttctcctg aaattacaaa gatgatattg tctaaacttt tttcaacttc
     7201 atttaaataa ctgcttatat tatatgataa atcagacaat agatttttaa tctgcttaaa
     7261 cacttgtaca tcaagtttag tattatactt ttcaaacaaa tctatttctt gattttctaa
     7321 tattcttggc atatatgctg acaataaatc taaattgttt ctttgttctt gtatttgctt
     7381 ataaagtgaa tttataactt ctattcttat ttcatcattt aactcttttt ctaaaatctt
     7441 atcaaattca gatttcatat agtaaatatt tccaaagaaa ttaaatatta tcatatcatt
     7501 catagcttta gaccaattct tatatatcac ttcaatagaa ttttctttaa gtagaggttg
     7561 ctttgcatat ataaatacat cttgcataag tttttgaacc atacttttac ttaagtttaa
     7621 aagtactttt tgattattaa attttatttc cgtttctaca aaattagaat tttcgttttc
     7681 aagttcttta ttaattacaa tgtcttttat atcactattt tttatagata tgatttcttt
     7741 ttgaaaagac tttagaatag tcaagctatc gaaatcttct tgaataaaaa atggagcatg
     7801 ttttaatgta tatcccacta tttcattttc tatatctttt aggtcatcta tttgcataat
     7861 ttctctattt ttatcattta tatgtaggtt aaatacattt ttattatcat ataatattat
     7921 atcttctcct aaatttaact tagaacaatt atctttcgct gactttacaa tagtcttctt
     7981 agatttctta tttataaaaa ttaattggtc ttgatatttt actaaaagca tgtttgtgtt
     8041 tttataatca aatccatcta tatttcctga tatcttgcag aatactattt cacaatcttt
     8101 aggaagattt cctatttttt ggttatcttt aactctctca tttaaatcag atgggactac
     8161 actatctata ttgttattaa atatagatac agaccttact ataatcccct ctctttccat
     8221 atgaaagtat cccttcaatg acactctttt atcttctaaa atgtatcttt ctatatcatc
     8281 aaaatttatt tcaaatctat ttttcccact gataaatata aacttctcat tatctttatt
     8341 aattccaccc atataaaact tattgtcatc taaacttaat atagtcatat tatcctttag
     8401 atagaatata aattctcttt gacttttgca gattaaatca tattcatcca aagttatttt
     8461 catagtcaaa tttgctgtaa catttttact tgaattttca ctttttatgt aaaaaggcaa
     8521 gttatttcca tagacaatta tttgagaatc ttctaaaact gcctctaatt tttctttatt
     8581 gtctgaaaaa ctatatatat attttacacc tgtatcctca ttaacttcat ctgatttgct
     8641 gattattata ttccctagat ttgttatatg aagcttttta tattcttttg agtatctact
     8701 tatgccacta agctcaaacc catctgaaag ctcatatatg attttttctc ctaatttcaa
     8761 cataccttca ccacctaaac agaattataa gtattttata aaggtgaata aaatgtctca
     8821 aaacaaataa tatttttttt aatacttttt attctgagac atagtttctc cttttttata
     8881 atgctctaag caccttcaac tatttcttca taaagtttat ctaaatgttt aacatattca
     8941 cgatatccag atttgttctt taactcactt cttaaatctt catatttttg tttaagttct
     9001 tcagcactca aagaaggagc tgattctttt aattcttcta acttataatc atagtactta
     9061 tcatacatag ctatatatgt atcaatagac ttcttagcta gttcattaca tttttcttgt
     9121 gcttctccat attgagaata atcacctatt tttattttat agtatccatt ttcttgaatt
     9181 attgttgaag aaataccttt agcttcatat tcttttgctg tttcttttgc tgttttttca
     9241 actttaagtg aacctgcata aacagtgtaa tatacacctt taccatcttt atatgcttcc
     9301 atttcttttg ctttcttttc agcttgtgct ttttcttgag cctctttttc agcttttgcc
     9361 ttttcttctg cttctttttt ctctttttca gcttgtgctt ttttctcttc ttcagttaat
     9421 ttattgttag cttgttgttg agttgatgac tttggaatat tctctttatc atttttctta
     9481 ttgttgccta ctgttataaa atatcctaca ccaacaataa ccactataac tactgctata
     9541 atagtttctt ttaatctact cttcttagaa acaacctctt catcatctaa agaatcttct
     9601 ttatctgata cataattttt catttctttc ttgttttctc tatatttgtc cttagaagaa
     9661 tttttagatt ttttattact attttctttc tttttaatag gtattactac tgatttttca
     9721 ttttctaatt tttcttcttt tatttctttt gcttttttat cttctaagtt tttactttct
     9781 ttttcaagct tcgattcatt atttgaagat actttttcat ctaagttatt atttattggt
     9841 ttttctattt ttacactacg ttcttcttct atatgttgac ttggttcttc ttgttttatt
     9901 tctttcaatt cattttgctc tatttcttct gatttcactt gatgattttc aactggttta
     9961 tctaaaatta tatctggaga aatatcattt aaattctctt cttcttgttc agcttcttca
    10021 ttgtcttttt taataaatcc aaataattta gccttctttt tactcttttt attttctggt
    10081 tcaattatat cttctttatt gctgtcgtac tctttatctg tagtatcttt atcttctaca
    10141 acgtcaccta tatcttgatt attactatct acttcttctt tagtcccttc ttcagcatca
    10201 tcttctattg aaatactact tctaaagcaa atatctttat ctgcattttt tatcatgtaa
    10261 atgtattctt caatatcgat atctttattt cttctagttc tttttgatgc aatttcatga
    10321 ttttctaatt cactatagtt atcttttatt agattctgtg ctttattcca tgtatcttca
    10381 ctaatttctg ttttattttt aattacatag tccacaaatt ccttaaaatt ttcaggacca
    10441 aattctccaa ttatagtcag tctcattgca ccttctataa ggtctctatt tcttctcttt
    10501 aaaaattcta atacaactgt tgcacaagca aaatctttaa taatttcttt gccaaaacca
    10561 tatgcttcaa cagaatgaag ttcttctaat ccacataact tcttatattc attgtgtatt
    10621 gtttgagata tactttcatt atttaagcta tttaacttat ctataaactt cgattctata
    10681 tcatttgatt ttgattcatg ttttactttt gaatttttaa tttcatttag tagcatgtca
    10741 ttttctgaat tatctttacc taattgcaaa tcctcaattt cagttgcttt tatttctact
    10801 ttgttttgtt cgttattctc ttccacatta ttaggtacca catctttatt actagctaat
    10861 attttgttta cacagaatat aatttctctt tttaattctc ttttcgcatc tgcttgattt
    10921 ttaagaaata aatctccaca ttctttcttt tctaaactta tttttctaag agatatatta
    10981 agaaggtctt tatataggtt taaaacctta taatcctttt ctaaacgtct tcttctctct
    11041 gatagttcca ttttatcata agctaaaaaa ctatttaaat cattggcttt tagagagtct
    11101 aatccctctt tcatagcctt atttacatac tttctacatt ttgctgtagt taactctaaa
    11161 tctttttcta attcatctat ttcaggtatg tacttatcta ctatccattt gccaataagt
    11221 ggttctgtat ttcttgctaa tgtcttataa aacaatatat taaattttaa aagctctgca
    11281 tattttgctt cattaaattc tactttttca aatttctttt ccaaaacacc atctagcttt
    11341 tcatttattt tagactcttg ttttaaaaaa cttgctgtga catttaaaac agtgtttctt
    11401 attatattat tttcactcaa aaccttcact ccttaaatca attattttat tgtaatattt
    11461 acattgtgca aagttgctaa tttgattatg atatctaaat aattatttac taaatttgct
    11521 acatcttata tcatatcata ttatttacat cattttactt tttcttttta tatctatctt
    11581 atattatacc atttttaata tctgtactaa cttatattac aatattcctc aaaattttaa
    11641 aagtagatta gacaaattta caaataaaaa aacatcagtt taaactgatg tttttgacta
    11701 tcgaaataat taagcaagtc tataagcgga gttctgtatt agataatcat ctttctaggc
    11761 ctattgtcac caataggctc aagcgaccta cctaccggag agtacgagca gcactcttta
    11821 ttcctatctt ggtcttgctc caggtggggt ttacatagcc aactagtcgc ctagctgctg
    11881 gtgagctctt acctcgcctt tccatcctta ccacaatagt ggcggtttat ttctgttgca
    11941 ctatccttag gatcgctccc actaggcgtt acctagcacc ctgctctatg gagctccgac
    12001 tttcctcatg taagattaca tgcgattatc tggcttactt aatatttatt ttacttagtt
    12061 atgttaacac aaattctttt acatgtcaaa gtaatattat actaaaaatt taaatttatt
    12121 caaaaatagt gtaatataga tatataaact acatttaact cgaggtgatc tattgaaaaa
    12181 attatataga atagtaatta acattatact agtattagta atactttaca gtggatttaa
    12241 tatatactca aaacttacca aatacaatca tgatactaaa atatcctcag aacttcaaaa
    12301 aaaagaatat aaaaaagagg atttatcaaa gattaacagt gattttaaat tttggctttc
    12361 tgttgagaat acaaatataa attatccagt tgtacaatct aaagataatt cttactatct
    12421 tgacaaagat ttttataaaa aagattctat ttctggtaca ctgtttatgg attatagaaa
    12481 taaatctatt gatgacaaaa atatcataat atatggacat aatatgaaaa ataaaactat
    12541 gttcaacaac ctaaataagt ttaaagatgc tgacttcttt aaaaaaaata ataaaataaa
    12601 aataaccttg aatggaaaag aatttttata tgatgttttt tctgcttata ttgtagaatc
    12661 tgattatgat tatctaaaaa caaattttaa caatgaatct gattatcaaa attacataaa
    12721 tgatattact tctaaatcat tgtataaatc tcctattaag gtaaatagca atgataaaat
    12781 agttacactt tctacatgta cctatgaatt tgatgatgcc agaatggtga ttcatggtag
    12841 attgatttaa aactcacaga cacagtttaa aacaataatt ttaaactgtg tcatctttta
    12901 tacctataat ttgtaaatta gaccactaaa agtatttaat acaaaataac aaatacatca
    12961 aacaataaaa aacaataaaa aaagactatg ctaaaaacat agtctttact ggagctggtg
    13021 ataggaatcg aacctacaac ctgctgatta caagtcagct gctctaccgt tgagccacac
    13081 cagcacacct attttattgt aaattagcaa aactaatttt taaaagtta
//
LOCUS       DAGSWI010000115        13117 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.115, whole genome shotgun sequence.
ACCESSION   DAGSWI010000115 DAGSWI010000000
VERSION     DAGSWI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.115"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            120..749
                     /locus_tag="MJF80_002936"
     CDS             120..749
                     /locus_tag="MJF80_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0281579.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            724..1128
                     /locus_tag="MJF80_002937"
     CDS             724..1128
                     /locus_tag="MJF80_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBZ0281580.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            1131..1604
                     /locus_tag="MJF80_002938"
     CDS             1131..1604
                     /locus_tag="MJF80_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBZ0281581.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            2151..2816
                     /locus_tag="MJF80_002939"
     CDS             2151..2816
                     /locus_tag="MJF80_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBZ0281582.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            2838..3770
                     /locus_tag="MJF80_002940"
     CDS             2838..3770
                     /locus_tag="MJF80_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBZ0281583.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            3791..4432
                     /gene="pcp"
                     /locus_tag="MJF80_002941"
     CDS             3791..4432
                     /gene="pcp"
                     /locus_tag="MJF80_002941"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBZ0281584.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(4552..4884)
                     /locus_tag="MJF80_002942"
     CDS             complement(4552..4884)
                     /locus_tag="MJF80_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281585.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            5149..5769
                     /locus_tag="MJF80_002943"
     CDS             5149..5769
                     /locus_tag="MJF80_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBZ0281586.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(6094..6918)
                     /locus_tag="MJF80_002944"
     CDS             complement(6094..6918)
                     /locus_tag="MJF80_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBZ0281587.1"
                     /translation="MDFLKKGISGFTIKILALIFMTFDHIAVFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(6929..8332)
                     /locus_tag="MJF80_002945"
     CDS             complement(6929..8332)
                     /locus_tag="MJF80_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0281588.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NTPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(8336..9061)
                     /locus_tag="MJF80_002946"
     CDS             complement(8336..9061)
                     /locus_tag="MJF80_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281589.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKVREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(9173..9946)
                     /locus_tag="MJF80_002947"
     CDS             complement(9173..9946)
                     /locus_tag="MJF80_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281590.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(9952..10839)
                     /locus_tag="MJF80_002948"
     CDS             complement(9952..10839)
                     /locus_tag="MJF80_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281591.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSGDNIQSVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(10853..11875)
                     /locus_tag="MJF80_002949"
     CDS             complement(10853..11875)
                     /locus_tag="MJF80_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281592.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            12450..12626
                     /locus_tag="MJF80_002950"
     CDS             12450..12626
                     /locus_tag="MJF80_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281593.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         4822 a         1894 c         1523 g         4878 t
ORIGIN      
        1 ttattatcca taaaaatata ttaacttata ttatttaata atttattgac agtattaaaa
       61 ttaaattata caataacagt gtaaattaac ttaacaccgt taagtaaaga ggtgattata
      121 tgagtatcaa agaaagacgt aaaaatgaaa aagaagaaat gaagaaaaag attatggatg
      181 ctagcatcga aattataaac caacatggat atgaaaattt atcaataaga aaaattgcta
      241 caaaaataga atattctcca actacaattt atctttacta caaagacaaa gcagagatta
      301 tcagcgatat gaccaataaa ctttataaca cagtagaaag taatgctatt gacattatga
      361 ataattgctc atcactacca atagataaac aaatcataga aattatgact agctttataa
      421 aaactctttc tagtgaacca gagatggcaa aagctatcat gcatagtaga atgaatatta
      481 tatttgcatc tgaaaataca aacaatacgc ctagcaataa tggtataaaa atgcttgata
      541 aattcctttc cattggaatt gaacaaggaa tattcaaaaa aaatatagat aaaagctcat
      601 ggatgcttat aagtgcattg ttaggattcg ttttatgtgt tgttgaaaat aaactatatt
      661 ctttacataa tttttctcaa cttatagaaa atttcttaga tattttagtg ggaggacttt
      721 acaatgaaaa ttcgcaacaa ggctactaat aaaaagaaaa aatatgtaaa aatatctatt
      781 gctctattct ttgtatttat atgcattatt attgtaattg taagcaactt aaagccagaa
      841 aacttggtag taaaagatat agatattaat aatgttaaaa atggcattta tactggaagt
      901 gctgataata acttggtaaa agctactgtg tctgttgaag ttaataatgg taaaatccaa
      961 aatattaata tcttaaaaca cgaccatctt ttaggtaaac ctgctgaaaa aattacaaca
     1021 agtattatta aacaacagtc tttagatgtg gatgctataa cttctgcaac ttatagcagt
     1081 aatactatac gaaaagctgt agaaaatgct ttaagaaagg gagaataatt atgaatacta
     1141 ttattatata ttcaagtaaa tatggttgta caaaagactg tgcaaatata ctaaaaaata
     1201 aactatcaga taatgtcact attgttgata ttaataataa taataaaatt gagttatcaa
     1261 aatttgataa aattattatt ggaagttcta tatatgttgg ttcagtttca aaaaaaatac
     1321 aagtgctatg taatgataat gtagagttat taaacaaaaa acaagttggc attttccttt
     1381 gctgtggctt ttctgaacaa gcagataaat atttaaaatc aaattttcca tcatccttat
     1441 tagaaagtgc aaatgctatt ggaatttttg gtagtgaagc tcgattagaa aaaatgaaat
     1501 ttttagataa acttattatg aaggctgttt caaaaggtaa ttatgacagt tttagaatat
     1561 cacaggataa tatagataat tttttaataa atcttaatag ctaaaataaa gctaattatt
     1621 tctatgataa attcttttat ttataagtaa tattgctcaa aatagtcttt aaactttatc
     1681 aaaattttat ttttcttagt gaaatctaat gaaactagca cttaatatat gttttattaa
     1741 tcataacaaa aagagagtgt ctaatgaact aaaaaattca ttagacactc tctttttgta
     1801 tgaaatcaaa tatattttta tcatttcaaa agagaaaaat acacttgtct ctatgtttgg
     1861 cacattctct ttttataata taatatctaa tttaaaacct atttcaaata ttgacatata
     1921 cttaaactat agacttacaa aatcaattaa tattagctta aaatataatc tttgttattc
     1981 aaattcctat acacaaccat tatttacttt gatactttaa aacatcttcc ctatttttta
     2041 ttaaatttta taataatagt cctattaacg caaatactat aaaaagagtt taactttatc
     2101 cattaaataa atttaaatta aaacatatag aaatggaagg agaacaattt atgataaaac
     2161 taatcggtat attgatagtt gtaattggat ttatacttaa aattgacaca ttatttactg
     2221 tattattagc aggagtggca acaggaattg ttgcaggatt agattttaat caaattttta
     2281 caattttagg agattctttt gtctcaaaca gaggtgtatc attattcata ttaacattgc
     2341 cagtaatcgg tgtattagaa agatatggtt taaaacaaag agctgtttct ttaatagaaa
     2401 aattaaaaag acttacaaca ggaaaagtac ttactatata catgatagct agacaaatag
     2461 ctggtgctct atcaataaga atgagtggac atcctcaatt tgtaagacca cttgttaatc
     2521 ctatggcaca agctgctggt ttaagtaata gtgatgaact aaaagaatcg gatgaagaag
     2581 ccataaaggc attatctgct gcatcagaaa actatggaaa tttttatggt caaaacttat
     2641 ttgcaggtag tagtggagta ttacttatag catctactct gactcaattt ggatacaatg
     2701 taactggact taatatcgta aaggctagta ctattatggc agtaatagca cttgtagtgg
     2761 ctacaatcca gtttacctta tatgataaaa aattaaacaa aagtcataaa aaatagtttt
     2821 atttaggagg aataaaaatg caaaatttta taaacatatc attggaaatc ttttacgcac
     2881 taatgggatt tttaatgatt gttatagcat ataagtcttt tactaccata aataacaata
     2941 agaaatatgg tacatcatta ttttggatac ttatatcatt gccattcata tttggtagat
     3001 tgattccagc caatataata ggaataatat taatattatc tagtttatta acactttcta
     3061 aacaagttgt atttgcaaag tatgaagaac cagatgaaaa ttttggaaaa gaacaagcag
     3121 ataaacttaa aaataaaata tttataccat cattaatatt agcatttgct gcagtagtag
     3181 tagctatgag cctttctaat tttgataatt cttctcaatt tgcaataggt gtgggttcaa
     3241 taattgcatt aatatcagcc cttataataa ctaaggcaaa acctgctaca tcagttcaag
     3301 atggctcaag attacttcaa caaatgggac ctgctagtat gttaccacaa cttttagttg
     3361 cattgggagc tttatttact caagccggag tcggtgaagt tatttcaacc atgatatctg
     3421 gtgtagtccc tgctgatagc agattatttg gtgttatagc ttatgtatta ggtatggtta
     3481 tatttaccat gataatggga aatgcatttg ccgcattttc agtcataacg gcaggaattg
     3541 gtattccttt tgtattgtct caaggtggaa atcctgctat aattggagca ttagcattaa
     3601 cagcaggata ttgtggaact ctacttactc caatggctgc taattttaat atagttccag
     3661 cagccttgct agaatgtaaa aatgattata ttgttataaa atatcaagct ccagttgctt
     3721 tggtattaat tatagcccac atattagtaa tgtacttttt aggattttaa tataattagg
     3781 aggtaaaata atgaaaattt tgttaacagg atttgaccca tttggtggag aaccaataaa
     3841 cccagctcaa gaagcagttg aaagggtcaa taataatata aacggtgcag aaatcataaa
     3901 gataactata ccaacagtta tgacaaaatc tgttgaagct atagataaag ctattcaaga
     3961 gcacaatcca gatatagtaa tatcagttgg acaagctgga ggtagatttg acattacacc
     4021 tgagagagtt gctataaata tagatgattt tagaataaaa gataatgagg gtaaccaagt
     4081 tatagatact attataaaag aagatggtga acctgcatat ttttcaaaat tgcctgtaaa
     4141 agctatggtt aaacatatga atgaaaataa aattccagct agtgtttcaa acactgcagg
     4201 aacatttgtt tgcaatcatg tgatgtatgg aattctttat atgatagaca agaaatatcc
     4261 taatataagg ggtggattta tacacatccc atatacaact tcacaagtaa tagataaaaa
     4321 aaatacacca ttcatgtctt tagaagaaat agtaaaaggc ttagaattag ctatagaagc
     4381 ttgtattata tataaagaag atgtaaaaga aattggtggt gaaatttcat aataaatttt
     4441 ttagtatttt tataaattaa aaatactaac tattaattta aatatagact gtgagtagat
     4501 tattcaaaca attttactca cagtcttttt atggtatcta cagatacaaa attaaggtct
     4561 attcctctct ccccactcac acattgcatc taaaattgga atcagagatt ttcccctttc
     4621 tgatagacta tattctacct taggaggtat ttgaggatat tcagtacgaa taatcaagtt
     4681 atctgcttct aattctttta atgataaact caaagtttta taggagatag tatttatata
     4741 acgttttaat gcattatatc ttacaacttc aaattctaca agacagtaaa gaattatcat
     4801 cttatattta ccactaatca gtgaaagtgt ataactaaat cctgtttcac ttaaatctgt
     4861 atttgatata cagtctatac tcatatatta cactctcctt ttggttagta ttgaacaaaa
     4921 atgtgcgtac ttgtttttag aataatttat aataaaatta tagtgtaaga ataattgaaa
     4981 gtcaatttta taataggagg tgtatttact aaaaaaaagt tattttaaat aggattctaa
     5041 gattagacat ttctatataa attgataata ttcatgaaaa tcctatttta taaaaaataa
     5101 tgtctcttag aaaatctatt aaataaaatt cattggagga aatttataat gaaagtttta
     5161 ttgataaacg gtagcccaaa ccaatatgga tgtacttata cagcactaaa tgagattact
     5221 aaagtattga gtaaacacga tattaaaaca gaaatactgt atttaggtaa agaaacaatc
     5281 ccaggatgta ttagttgcgc atcctgtttt gaaactggaa aatgtattcg aaatgataaa
     5341 gttaatgaat tgattgaaga tctggataat attgatggta ttattattgg ttctcctgtt
     5401 tacttttcta gtgcaactgg tcaattaact tcttttcttg accgtttatt tttcatagct
     5461 gggagtcgca tggctacaaa attaggtgct tcagttgtgt catgtagacg tggtggtgca
     5521 agtgcaacat ttgaccaact aaataaatat ttttcaatca gcaatatgcc tattgtatca
     5581 tcacagtatt ggaaccaggt acatggcttt actccagagg atgttatgaa ggatgaagaa
     5641 ggattacaga ctatgcgaac tctaggagaa aacatggcat ggcttcttaa gtgtatatca
     5701 gcaggtaaaa aagctggaat taaagaacca caatatgaag aaacaattat gactaatttt
     5761 ataaaataaa taaaaaatgc actaatatta attaaaacaa aacactaatt tataaataaa
     5821 ataagcataa atgtaggata aaaagcacta aaacagaggt attgaatttt atatcaaacc
     5881 tctgttttat tattacatta ataaccaaac catatataac atcatatatt tctaaataaa
     5941 ttataatatt atattctaat gttattgaaa ttacaataac tgaatttctg ctcataaatt
     6001 taaatattct acaaatctca tctaaataac aattttaata gacctaaaac aatcctcata
     6061 ttgttcattt tgtattaaaa atatcactta caatcaaaaa ccatttatta agtatattcc
     6121 taagatatac aatatatata catggattgg atagtataaa taaaataaat acttcatact
     6181 cttccctttt tctccattgt aaagtaacat taaaggtaga gaagcaatca tcatccattg
     6241 gtaattttgt aaaatagaat tttttaaact ataatctcca tttgacattg ttgtaaatat
     6301 aaatatacac attaaaacat aacaaatact caaactgaac tttttacctc tacacaaata
     6361 aaaaataatc ccaagtataa tccaaattgg tccaccttct actaaaaatg gtactggtac
     6421 caaaatcata aataaagcaa taactctgtt agtgacttta aaaagtaaag ctactgtaaa
     6481 tattcctaaa attataggaa tgattatcat caataatcct ataataaaat atttaatttc
     6541 tttctctttt ctaaatttct ttatgaactc tattgcttgt aaataaatcg ctattaaaaa
     6601 taatgttgaa aaaatgttat ttataattat agctccttct ggatgtgaaa atacattatt
     6661 aattatttga tttcctatag ccataataac agaccaaata tacaatctac ttatatattt
     6721 ttttctgtta ctagtgtgat aaaatccttc aacagccatg aatataaaca gtggtgctga
     6781 tattctgcct acccaatgaa accaaattgg tatttgcatt gtttgtggca taaatactgc
     6841 tatatggtca aatgtcataa atattaatgc taaaatctta atagtaaacc cacttatacc
     6901 tttttttaaa aaatccatat catatctcct aattttgttt tatctctata ataatttttg
     6961 ttcctattcc aatttcacta cttacataaa ttttaccttt atgaatctct attatttctt
     7021 tagaaatagc cataccaaga cctgaacctt tatgcatctc acctgtatta gtacctctat
     7081 aatatttttt aaaaatatgt ttcaaatcac ttttgcttat tccaattcca ttatctttta
     7141 ttataatttc tatattatct ttttttacaa tttctacact tatcaaagtt ccttcgctat
     7201 tatgaactat tgaattaaat ataagattgg ttatagccct tctgaataag attgaatcta
     7261 catatacttc aattaaatct tcattacttt caaattcaat attcctattt ttatatctat
     7321 tatcatttaa aatatcaatt attatatctc tgactaaact gactaaattt atttttttct
     7381 tgtccagaac tatagtatta ttttttaatc gagtagataa atttaaatca tcaactaaat
     7441 cttttatata cttagactta ttatatataa tttctgtata ctcttgtatt tcatcttctt
     7501 caaactcata atctttgtca ttaattatct ctgcataccc ttgtatagat gctagaggtg
     7561 tctttatatc atgtgttata tttgccagcc attcctctct caactcttca ttttcttttc
     7621 tctcaacttc atttactctt aaagtatcag ctaacatatt tatattttta aaaacttctt
     7681 catatatacc tctgtcctta agatataaac tataatttcc attagccata gtctcaatac
     7741 tccatagtac gcttgaaatt ggttttgtaa gttttctact aaataaatat cctacaccta
     7801 aagctaccac gctatcaatt actagtgtta taatgataac tttatttaaa gtttttttaa
     7861 tgttattttg agaataggtt aaaattattc ttgatatatc tctactaggt atacctacta
     7921 ttatgtttag atgttttttg tttaaatatt tttctagtac aaaattaaca tactttgttt
     7981 ctatatactt gtaattattc accatttgaa agggtgtata ttttttggga gtatcctttg
     8041 gcacatttac tccataaact tcctctccta aatcatttaa aacctgtatc caggaatttg
     8101 atttttctaa tttttcagct ccaatcttag atagtttaaa ttctccatct ttttcatata
     8161 aatctttttc aaaagaacgt gcaaattctt caggagtatt tccagaatct acaactttaa
     8221 aaaaactatt agttgaaatc acatacaata tagatatgat atttataatt actactgata
     8281 tagctacaaa cacaattgta aatataaaat ttctagtaat cttccacttc atattctaat
     8341 cttcctttac tgctaattta tatccaagac cttttattgt cagaatatat tcaggctttg
     8401 aaggattatc ctctaatttt tccctaactt ttcttatatg aaccattata gtattgtcaa
     8461 atccaacata atcttctccc cacactttat cccatagttt ttctttactt ataatttgat
     8521 tttgattatg tgcaaataaa gataggattt ttagttcctt tgcagacagt cctatagatt
     8581 ttccatcttt tataagttct gccctacttt catttaatat atatgggcca aatgatataa
     8641 ttttttcttc attattattt agatttttac tttcgttttg agcatcacta aaataactta
     8701 atcttctcaa atgcgcttta actctaaaag ccacttcctt agggctaaaa ggttttgtaa
     8761 cataatcatc tcctccaatc gctagtccaa gtaacttatc taattcttcg gttttagctg
     8821 ataaaaatat tattggagaa ttggaggttt ttcttatttc tttacaaata tcataacctt
     8881 cgccatctgg taacataata tccaatacaa tcaaatcagg attaatactt ttaaataatt
     8941 ctaatccttc ttttttagtt tttgcagtat atatattatt aaggccctct ttttttagga
     9001 cagtttctaa aagcttcaat atttctactt catcatctat tattaaaaca cgattttcca
     9061 taaacaacct cctgtaataa tttacatata tattagcata tattttatct aaaattcatt
     9121 tatcttattt ttaattaaac taaaatacaa ataaaattac tattattttt ggttaaaacc
     9181 ttttgcttga tgtaaaattt tcatacgtta ttttagttaa cactatcatt aatataattg
     9241 agattactat agaaaaaact atcaaaatac ttggtacatt atacttaacc atttgttggc
     9301 tcatcataat ccaatttgga gtcactgaca taaattttat agctttatct ggtataaaac
     9361 ctacactaaa gtatggaatt gccattatca atccacctag tataaatgga attacagccg
     9421 atttaaatat agatgaaagt gttaagccta tttgtgcaaa tataaaagaa gcaaaaatag
     9481 tagtcaatat aacaattata tacatttgta ttactgtaag atttaaatta ctatttccaa
     9541 agtaccataa gttatgaatc gattcattta aatttttaaa atcaaaaaaa ctaaaacctc
     9601 taattcctac taacaatata aaagaaatat taataataga taaaattaat ccattagcta
     9661 ctagttttga taatactact gatttttttc cctttttagt tgtatttatt aaacctgtca
     9721 tattagaatc atattcttta atgtaatttc cagaagttat tataaacatt atcaaaccta
     9781 ttagaggtat tgagccttct atcttgtaaa atatatctgc ataagtatct aaaacatcaa
     9841 actttctaat cagaaaaaat ccaaaaaata ttaaaaacag ccaaattatc caaacactct
     9901 ttgattttaa tatttttatc atttcagctt taactaaata catcatttct actacacctc
     9961 ccttgcatct tccaaatcaa aataaaacat gtatacatct tcgaattttg cttgaactga
    10021 ttgtatatta tccccacttg gaatatcctc tgaaacaatt ctaagtacta ctgaatcaat
    10081 atcactttga atacttacaa ccttatattt agattgtata tcatcaactt cacattcatt
    10141 tttaacttga acattatata ctttattttc catgtgactt aatatgtctt tgtgtgttcc
    10201 tttcattaga agtttgccat ctttaatcat tattgtttcc tttgcaactg attctatatc
    10261 ggaaattata tgagtagaaa gtattattat cttatctcta gacatttttg ctattaaatt
    10321 cctaaatcta gctctttctt gtgggtcaag acctgctgtt ggttcatcaa ggactattat
    10381 cttagggtcg tttagtaaaa cttgtgctat cccaactctc ctcttcatac ctcctgaaaa
    10441 ttttcctata gacttatttc tcacatcata aagacctact aaatctaata attctcctat
    10501 cttactattt aaatattttc cccttaaacc ttttaaagct ccaatatact gtaaaaattc
    10561 ttttgctgta aaatttttat aaactccaaa ttcttgtggc aaatatccta aaatatttct
    10621 ataatcagca tccattttaa atatatcctc accattataa agtatctgac cttcatcagg
    10681 ttttatcaac gttgttattt gtttcattaa tgtagatttt cctgctccat ttggtcctag
    10741 aagaccataa actccatttt ccagagttac tgtaatcttg tcgtttgcta cttttttacc
    10801 atatatcttt gaaagatttt ctatttctaa tttattcata ttaactcccc ttttatctac
    10861 ttatataatc tgattttatt actaaaaaac ataatgacat agcacctatc aatattgcca
    10921 tcaaaagtcc tattgctata ttgacaatgt ctatattcac tttaaatatc ataatatcac
    10981 ctagataatt tcctattatt ttattaaaac caaatatcaa atcataataa tttacatttg
    11041 atacaactga aagtaatttt ttaggtaata tatggattac cgacattatc ttagttatag
    11101 atataataaa tataaaaaaa cttattgaag atattgaaga cttacttata aaagacccaa
    11161 atatggctat catggcccat ccttcaaaca tcaaaagctc tatgcctata tttaatatta
    11221 catactttgt tatacttaaa ttagttttca atgcaactac attgtctact attctaaaag
    11281 attctaagtc tccattaaca ggtactcctt gttgcataca agttataata aatattccca
    11341 ttaaataaac agaataaatt actaatggaa ctaataaagc aacaaatatt ttagacttta
    11401 atgctttttt cttctctttt gatgatagta tgatatcttt taccgaagta acgacttcat
    11461 ctacataaat attagatata attatagata taatgattaa cattcctaca tttataagtg
    11521 aatttgtaac tctatagtta aatactttcc aaaaactaat atccttatat tcattcacac
    11581 ttagattatc aagttttttc tttgacatta attcaatttt tttagaaaat tcacctgtat
    11641 ctgtttgatt tgaaaactgc tttaaaacat taatcttatt ttgaaatttc tcattaccta
    11701 tgtctatacc acttctcgta tcttctatac gtcctttttc tttatctata taataacttt
    11761 ctgtttctaa aacaggcgta ataaagatac taatcattag tattaacact aatatcccta
    11821 gagatataaa catactctta gatttagcca atttttttat ttcccaattt accaatatat
    11881 ttctcctcct aaatcatttt aattacacat atatattaaa acttaaatct tatataaata
    11941 taatactaac cttaaataaa gcttaaatta ttttataatt tataggtgtg tttattattg
    12001 gagttacatt tattattttt ctgggaaatt atacattttc aattattttt attatttttg
    12061 atatttttat tgattggaaa ttttattgtt gttcaaattg tagttattct ttaaatttaa
    12121 agatggagtt ttttaagaat agaacctact gtccttatta tggaaagatt attaaatagt
    12181 tgcatataac tttatttatc ttgaatctta tttttaaaaa caattataat tttagaatat
    12241 tttctattct caaacaagca tgttgaataa tggagcatct tctagtatta tatacttgta
    12301 gaataaaact aaattggtaa aactgggaat atttaacctt aaggagttaa gttatagaaa
    12361 ctagtactaa aaaattttat aaattatgtt aattgactaa tagaatgcga ctttttattt
    12421 ttactaaatt ttttaggggg atggataata tgtttattgg agaaatagac aaatgtacac
    12481 atatactaac tgcttacatc agtagttcat atgattattg taatttttta gatacacagt
    12541 tagatgattt tatatcagaa tatggagaaa cggtagttga aacctgctta catcaagtct
    12601 tattattagt aagtagatac aattaaaact aaatttttct agaaaaacct ttttagattt
    12661 atctactcta ataaatatat aattttttgt actttataag taactataac ttctgctaaa
    12721 gtaaagatgt attgggagta catctttact ttcccattat agaagttaaa agcatacacc
    12781 ataaaattaa tcatccaata caaattatat atttcctatg tctaacttaa tttacaagaa
    12841 tcattctata tgcatattct gcaatccact aaacatggct ctaaaccttt actattttat
    12901 acattctcta taaatcatac aaatcatata taaaatcccc ctaatatgtt taaaccacct
    12961 ataaaataac accaatattt agaatattca atatcaactg cataactggt caataaaaac
    13021 gtataactag acaaatttca atatttatat aaataataca agaaaaacaa gcattttatt
    13081 tatataagtt tttcattttt tgtttatatt aaaaaac
//
LOCUS       DAGSWI010000116        12884 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.116, whole genome shotgun sequence.
ACCESSION   DAGSWI010000116 DAGSWI010000000
VERSION     DAGSWI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.116"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(39..476)
                     /locus_tag="MJF80_002951"
     CDS             complement(39..476)
                     /locus_tag="MJF80_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBZ0281594.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSIPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(733..882)
                     /locus_tag="MJF80_002952"
     CDS             complement(733..882)
                     /locus_tag="MJF80_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBZ0281595.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(895..1125)
                     /locus_tag="MJF80_002953"
     CDS             complement(895..1125)
                     /locus_tag="MJF80_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBZ0281596.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(1942..2163)
                     /locus_tag="MJF80_002954"
     CDS             complement(1942..2163)
                     /locus_tag="MJF80_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBZ0281597.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2743..3000
                     /locus_tag="MJF80_002955"
     CDS             2743..3000
                     /locus_tag="MJF80_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBZ0281598.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            3469..4707
                     /locus_tag="MJF80_002956"
     CDS             3469..4707
                     /locus_tag="MJF80_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBZ0281599.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYITYDG
                     SVRERKKYENVRYWNPICEI"
     gene            5491..6792
                     /locus_tag="MJF80_002957"
     CDS             5491..6792
                     /locus_tag="MJF80_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBZ0281600.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            6868..7143
                     /locus_tag="MJF80_002958"
     CDS             6868..7143
                     /locus_tag="MJF80_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBZ0281601.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            8478..8708
                     /locus_tag="MJF80_002959"
     CDS             8478..8708
                     /locus_tag="MJF80_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBZ0281602.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            8701..9282
                     /locus_tag="MJF80_002960"
     CDS             8701..9282
                     /locus_tag="MJF80_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBZ0281603.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(9861..10538)
                     /locus_tag="MJF80_002961"
     CDS             complement(9861..10538)
                     /locus_tag="MJF80_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBZ0281604.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(10727..12091)
                     /locus_tag="MJF80_002962"
     CDS             complement(10727..12091)
                     /locus_tag="MJF80_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBZ0281605.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            12219..12740
                     /locus_tag="MJF80_002963"
     CDS             12219..12740
                     /locus_tag="MJF80_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBZ0281606.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         5006 a         1391 c         2054 g         4433 t
ORIGIN      
        1 tgcaattagt gatattaata aattacttta gatttttatt aaatgtttag cttttcttta
       61 atatttttta tataacgacg atttataata actttttcgt tgttttttaa agtagcttgc
      121 atgcgattat gtgatattac ttttatactt tttatgtagt ccatatttaa aatcatggat
      181 ttagatattc gtgagaaaga tgtattagct aataattttg ataatttata tatttttaaa
      241 tcaatttcaa atacatcatc ttcataataa gcaaatgttt tatttccaac tgtctcaaag
      301 tataaaatat cacagacatt gagtttataa atctctcctg attttttacc ttttatatca
      361 agtaacggta tactcaataa tgaaattatt ttttcaatgt tattatctag agcagaacat
      421 tttatggtta tttcaatatc atcacaatta ataccttgtt cgacatgtat tttcattaat
      481 catcacctca ttttattact gtcattagaa tagcattata tcttattgag tccaactaga
      541 tgcgttctaa atttcaaatt aagcatagtg aaattcaaaa ataaaacagt gaaattcaaa
      601 tttgaggtgg taaaattcaa aaaaatacta tggatatttg gataaattat ccaaatatcc
      661 tagtatttca catagaatat gtctaattat tttacaaata gacctatttt atgacttaca
      721 tatattgtca aattacttct taaatatcaa aatatttttg gataatgtat caatactgtt
      781 tgataaagta tttaatttac cctcaattct aaccaacaag tacatggata gagctatagg
      841 aaaaccaaca ttggttatta acagttgtaa actatcatcc ataaaattag ccccctattc
      901 tataactaag tcataaggag tggtatttgt ttccacaacc ttggcttcaa caacagaagc
      961 taggtcaaat cctccaaccg aaaaaatatt tttagatact atcaaatcca tgacagattt
     1021 tatattttct tcagttaaat catcctttgg attttctata gaaaaagaaa cttgtttatc
     1081 ctttacattt ttaaagctca tgactaattt tttcttaatt tccatataat tacctccgat
     1141 ataataattt atattttaat tgtttgcttg ttaatattgg tattttaatt atcaatatta
     1201 atgttttgct tgttagtatt aatatttcaa ttgtcaatat taatgttttg cttgttagta
     1261 ttaatatttc aattgtcaat attagtgtct tgcttgttag tatcagtgtt tcaattgtca
     1321 atattagtgt tttgcttgtt agtattaata tttcaattgt caatattagt gttttgcttg
     1381 ttagtattaa tatttcaatt gtcaatatta gtgtcttgct tgttagtatt aatatttcaa
     1441 ttgtcaatat tagtgttttg cttgttagta ttagtatttc aattgtcaat attagtgttt
     1501 tgtttgttag tatcagtgtt tcaattgtca atattagtgt tttgcttgtt agtattagta
     1561 tttcaattgt caatattaat gttttgcttg ttagtattag tatttcaatt gtcaatatta
     1621 gtgttttgct tgttagtatc agtgtttcaa ttgtcaatat tagtatttta attgttgact
     1681 ctagttattg ctgatataga aaatatttaa tttatttcaa ttaattagat taaagtaggg
     1741 tagcatctaa aatagctaac atgtttaagt tgaaatacgt ttaatatatt aatttttcaa
     1801 agcgttaata tattaaagtt ataaccaaaa ttgaagttat aatctgccaa atctatagcg
     1861 tattaagtgc attatataac ttaatctttt atataaagag ttaatttttt aatttgtaaa
     1921 gcagagtacg tttaaaagac actatttaga tagtgatgta ctatctaatt ttaaaatatc
     1981 tacaataggg ttctcttgaa gacttgctat tgcttctgca acagcataaa catcttcatt
     2041 taatgcctct gcttttacat ttgcaaatga tttactttta acaactgcct tacccttatc
     2101 atcaaaacca gcatccaatt ttagttttat actagataaa ttttttgttg aaacaacttc
     2161 cataaaatta cctcctaaaa atttaatttc aaagatttgt cgactttctt tgttagctat
     2221 aatactatca tctaagataa tatcggagtt aaacagtaat ttctaaagat tttttgtgta
     2281 aatttttata ggaaaaattt tacttttttg ctgatgaaaa agtttacaag ttatttgtga
     2341 agatatgttc ttagtgtttt gaaatagtat ttgtatatat tcatgcttag ttgagatagt
     2401 tttgtatgga ttttcatatg tgtagagtta aatgttaggt actaaagttt atgttaaatt
     2461 aaatgttaaa gcttatgtta aaaattttat taaatttaat ctgtaaataa aaaagttaaa
     2521 agtccaatat taacatttta cttttaaacc attaaaatct ttattaaagc taacaaagtt
     2581 ataagttatg agaaatttta ttgaagttaa tacaaaaatt aattttaaag ctaagaaatt
     2641 ttttattaga ttcaataaaa ctaataaata tagtttagtc aataatgtat taaataaaaa
     2701 ctttgttaag tctataaaaa tatcttaaag gaggtgtaac ttatgaagat aaaagtggag
     2761 gatataccag ttcaatttca tgcaatggta gaaataatcg gaattgataa gttcatagag
     2821 gttgccaagt tgtatggagg aacaaatact tatataccta cgtataaggg gctttttaga
     2881 tatgctagaa atagggatat agtaaaacaa tttaatggag tcaaccacaa tgaattggct
     2941 attagatata atatgtgtgt aagcaatatt aagcgaatac tcaatgaaaa tagtgtttaa
     3001 atataataaa ataatgttta aacataatga aaatagcctt gttaaacgta agagtaaaga
     3061 caaagaagat gttttataaa gtttttttag acaaattaac taaatttact acttattaat
     3121 ttaaatacat tgtatattct ataaaaaaat ttgttaaata ctggagatat attgagaata
     3181 caaagaaaat ggtcgaaaat atttgttggc taaaaaatag ataaaatgat acaattgctt
     3241 gtataaaggg gaaaaataaa taaataaact atataaataa agaaatagca attaatatga
     3301 cataatatat ttataaaatt aagaatataa atagatattt taagggatta gttgttaaat
     3361 taggaaatat taaaaaaaat atatatttat atttaaacta ttttgttatt tatataaaaa
     3421 tacttatcta gttactagca attattagta gaaatggggg aaatagagat gaataaggga
     3481 ttttacaaat taaattcagt ggtattgaac tttgatatta agaatgatgt taacaagata
     3541 agatttaatc attatataat gagaaaagaa aacatacaaa gacttcaagg aactctgcca
     3601 aacggacaat tttatatgac agtgagaaga gctgctacag atttacaact ttctatttct
     3661 actataagta ggcttgtaaa agagtttttg gatttaggga taataagatt agtgtctaag
     3721 ggtgtaaaag gtaatcgttc tgtatatagt tacgtaagta tagagtttaa tggaagttta
     3781 tttgatgata gtaaatatat ggaacttgac tgtgtatgta atgatgaatt tattgataat
     3841 agtggcatag agtttgattg tgatagcaaa gataattctt ttaatggaga tataaatgta
     3901 gaaactgtgc attgcattaa tgagtatttc tctgatgatg atgtagagca taattataac
     3961 aatttccata acaaagatac tataaaggat aaagccattt ctaatattag cactatcaaa
     4021 aaatatattg atgaatcttt tgatacaaat aaatcagaaa attctaactt acaacaggag
     4081 tcaataaatt tcaactttaa tgatacatgc aatgaaatag ataaaagaac gaataatagt
     4141 atagaaaata atcaaaataa aaggcctatg agattgagtg acatcagtct tttaagtgat
     4201 aaaggtatga caaataaaaa gaattacttt aatcataatc atggtataca aaaagggact
     4261 aaatctgtaa caaagaaaaa agaatcctta aataaattta ataaaaaaaa tatacagcaa
     4321 agcattataa atatgctaaa taaaaaatca ggtaagaatt ttaaaatcga ttctccaata
     4381 agcataaaac ttatcaatga aaggctaaag gaaggctatg tcttagaaga tttctacaaa
     4441 gttattgagg taaaagtggc taagtggaaa aataccataa tggaaatgta tataagacca
     4501 gaaactttgt ttagctataa gtttgaatcc tatgtaaatg aaaacatatc tatgaaaagt
     4561 caaagtaatg ttcacccatg taaagaaggc aatagtaatg atagcagtaa ttatatacat
     4621 tctgattata taacatatga tggcagtgtg cgtgagagaa aaaaatatga gaatgtaaga
     4681 tactggaatc ctatatgtga aatttaatta gtttaaaaat gtatttatga taaataagat
     4741 attaatagag aatagtgagc tatagcaaat gatatatcaa tagttggtag taagttatat
     4801 taagtaagat attactatca ggtagtgaat tatattgaac gatatattaa taattggcag
     4861 taagttatat caaataagat attactatag agtaatgaat tatattgaac gatatattaa
     4921 taattggcag taaattatat taaataagat attactatag agtaatgaat tatattggac
     4981 gatatatcaa tagttggtag taagttatat taagcaagat attactatca ggtagtgaat
     5041 tatattgaac gatatatcaa taattggtag taaattatat taagtaagat attactatca
     5101 ggtagtgaat tatattgaac gatatattaa taattggcag taaattatat taaataagat
     5161 attactatcg agtaatgaat tatattgaac gatatatcaa taattggcag taagttatat
     5221 caaataagat attactatcg agtaatgaat tatattgaac aatatattaa taattggcag
     5281 taagttatat caaataagat attgctatta tgtagtaaat tatataaaac aatgtatcag
     5341 aataaatagt atattaataa tttgtagtat atcatgacga ataatacagt cataacaaat
     5401 agtgagtcat aacaaataat gtatttataa ttgataatgt atttaaaaat caaaatataa
     5461 aaacttataa ggaaagagag attaatttat atgggtaaat caaaagatgt tgaaatagag
     5521 aagtctattt taggcacaat tcttttagac aataaattaa gctataagct agatgaatta
     5581 aatgaaaaca tgtttatgaa tgatatatgt cttgagatat ttaaaattat gaaagaactt
     5641 aaaaaagaaa acatagtgat tgatgtggcc actgtgaaat caaagataga tagaaaatct
     5701 ctagcaataa aaacaagtga tgtgactaat ctaattactt ggggtcagaa ttttggtctt
     5761 gatggtcata ttaaaatttt aaaagaaaat ttagctagaa gaagcataaa tcaaaactgc
     5821 caaaaccttt tacacagcct aaaccttgga gaaaatatcg atacttgtat ttacaaattt
     5881 gaaagtaata taaaggagat tttggacaag gatacttatg aaaatgatga tgtaaatagt
     5941 atagcaggga aggtgcttga ttttttagaa aacaaaaaag atattggatt taaatttggt
     6001 ataaaattac ttgataccac tataggaggt ctttttaaag gagagctaac cacaatagca
     6061 gctaaatcgg gggttgggaa gactgctttg gcacttcaaa taatgttaaa ttcatttaag
     6121 caaggtaaaa aaactttatt tataagtaga gaaatgacca gtgagcaagt atttatgaga
     6181 aatatatgca gagtaactgg tgtaagtaca aggaatatga aatctaaaga aatagatgaa
     6241 aatgattgga aacttattgt aaatgcgata ggtgatttaa gcgaaaataa cttaatttat
     6301 atcaatgata agatagatac aattagtgca attagaaaga ggataaggca ggtaaagcca
     6361 gatttattaa tagtggatta tgtgcaactc cttacatctc agaaaagcat ggataagaga
     6421 gaaagggagg tagctacttt ttcgagagaa cttaaaaata tgactttaga ttttaatata
     6481 cctgtaatcc aattaagtca gttaaatgat gaaatgaaag attcaagacc ttatggagat
     6541 agacctatga gggatagcaa agccatctac cacgatagca acaatgtggt ttatatacat
     6601 caattaaagg ggagtgatta tgaagaagca gtgagagata ttggagaaag tgaggaggca
     6661 gttagagcat cagaatacag gggaattaag atggttgatt taatagttgc gaagtgtaga
     6721 gatggacaga ctaggcataa gcatttttgt tactttgggg ataagttaca ttttcaagaa
     6781 ctaaattatt aggtcaataa agtagtaata acaagcttat taaaagtcgg gaaatagcaa
     6841 atagattata aaatatgagg tgaaaatatg ttggaattaa gaaaaattat agaattactt
     6901 gaagaacaca agatagaagt aagtattcca aaggagttac ataagtatag tttattggaa
     6961 taccttgaat tgtgtcaaca aagtggggga acatattttt atattaaaac actggaaggg
     7021 gctttaaaag aggctagaaa tgagcttatg attaaattgt ttaatgggaa aaattatatg
     7081 gaattagcga ggatgtttga aatgagtgtt attaatgtca ggcatattgt tagaaaaaaa
     7141 tagatatttt tggagaatac aagtgtgata tacttctgaa atagaagtta ttataagagg
     7201 agtataagtg tgattaacaa catgattttg tgaataaaat tgaggatata tatgagttaa
     7261 atgtgtgatt aataaggtaa gattgtgtat aagattatga gtatttaaaa ggaggggcta
     7321 taattaatag tgtagagttg tgtgtttgct taagattata taataattta atagattaaa
     7381 ctatactaat aaggtaaaat cgtgtgttta gttaaagctt attataaaat cttgatatgt
     7441 gattaatgat gcaatatgtg aataaagtta agattataat agatttaacc tgtggctaat
     7501 agaataaaat tgtgaataaa gttaagatta taataggttt aaattgtgac taatagagta
     7561 agagtgtaaa tagagataaa cttcaatatg agcttaacct atgattaata gagtaagaat
     7621 gtaaatagag ataaacttca atatgagttt aacttgtggc taatagagta agagtgtaaa
     7681 tagagataaa cttcaatatg ggtttaactt gtggttaata gagtaaggtg taactaaaga
     7741 taaacttcaa tatgagctta acctatgatt aatagagtaa gaatgtaaat agagataaac
     7801 ttcaatatga gtttaacttg tggctaatag agtaagagtg taactaaaga taaacttcaa
     7861 tatgggttta acttgtggtt aatagagtaa gaatgtaaat agagataaac ttcaatatga
     7921 gcttaacttg tgattaatag agtaagagtg taaatagaga taaacttcaa tatgggatta
     7981 acttgtgatt aatagagtaa gagtgtaact aaagataaac ttcaatatga gcttaaccta
     8041 tgattaatag agtaagaatg taaatagaga taaacttcaa tatgggttta acctgtggtt
     8101 aatagagtag ggtgtaacta aagataaact tcaatatgag tttaacttgt ggctaataga
     8161 ataagattgt gaataaagtt atagttttag atgggtatta acctgttact aatagaatat
     8221 gattaggtgt aaaattatag agctgcataa gaaaatatta tagatatatt aaatattaga
     8281 taataaatat ttatatagat acacaaaaga aaaggcgata tgaagctaaa cttaatgact
     8341 tcatatcgtt ttttatttga aactcctttg tatttagagt gtgattttta atattgaggt
     8401 tttatgtgat taagtcacag ataaaaagta agtatcttga tataattctt atagattaat
     8461 aatataggga ggttattatg tctaataaaa aaaagaagta tgcaaatatt ttagcaaatg
     8521 agtgcgttgc ttgtggtagc tgtataaaag catgtccaag aagtgctatc tcagttccat
     8581 gtggcattag tgcaaaaatt aatagagatt tatgtgttgg atgtgggatt tgtgaaaaga
     8641 tatgtccagc atctgtaatt gagattataa caatattaaa agaggaagaa ggtaagtgtc
     8701 atgaataata aaaaacattg gtatgactat ttatggattt tttcttcaat atatttgata
     8761 ttgggatttg taaatattat tttcgcttgg attgggctaa tttgtttctt tgttccacta
     8821 gctatttcta ttgttaaggg aaataaagct tactgtaata agtattgtgg aagagggcaa
     8881 ctgtttaatt tgcttggaaa taaattgaag ctgtcaagaa atagagatat tcctaaattt
     8941 attagaagta agtggtttag atatgggttt ttgacatttt ttatggtaat gtttgccaat
     9001 atgctttttt caacttattt agtgtttgaa ggaagtagaa attttaaaga agtagtgact
     9061 ttgttgtgga ctataaaaat gccttggcaa tgggcgtatc atggaacttt agtttcacct
     9121 tgggtggtac agtttgcatt tggtttttat agtgttatgc ttacatcaac tgttcttggt
     9181 ttgattacta tgatattgtt taaacctcgt tcatggtgtg tttattgccc tatgggtact
     9241 atgactcaga tgatatgtaa ggcaaagagt aagtctgtat agaaaggcct tatgtgatat
     9301 ttacagtttt gttcaagtag agatgagtag taggtttgaa tatatatgac aaatatatga
     9361 gtataggggc atagatttag tttgtgtaag gtaaactgat ttatgcttct tttttatgtc
     9421 ttagattatt ggctaagtgg aaacaatgaa tttagatatt aagaattatt cagttatttg
     9481 aagaaaatag aataataatt aacattatat taatggatat tcattacata taagccagtt
     9541 aatagcaaga tttatttaat atgatatagg tcaagtattt aaactaaata aggtcatgac
     9601 aattaatata agtatagata taaaaagatt agaagttata acttataaaa ggtattctat
     9661 ataaatagta tggggaaaat agtcaaatta acttattaga aacaaaaaat atacataaaa
     9721 aataaatgta aatatcaaat aagatactat atcaaaatag atataagtat cttttttatt
     9781 attgaaatta tgaaaatata tttgatttta cacaaaaaag agtgcctcat gaactaaaaa
     9841 gttcattttg agacacactt ttacagtgtc aaagaacgaa gtttatcctt atccaatacc
     9901 tttataccac cacgagataa agacacaata ccttctctag caaaatattt caacattctc
     9961 gatacaactt ctctggcact acccatatac ttagcaattt gctcatgagt catatttata
    10021 gtgctactgc catttttagc tatctcatca attaaaaaag atgccaaacg cttatcaaaa
    10081 ctcataaaaa gaatttgttg catagcccac ataacatcag aaaaacgttc agtagctaat
    10141 ttatatgaaa ataactctgc atgaatattt tctgtagata attttgcaaa aacagaggaa
    10201 ctaatctgta taatatcgca gtccgtttca gcatctacat agacatcaaa agttattgtt
    10261 tttagaatac aggaagctga taaaatacat acatctccat catcaagtcg gaacagtgta
    10321 acctctcgac cttcatctga aagtatgtat gtacgcactg ttccagattt tataatcaaa
    10381 ataccaatac agtcactatc accacaatgt actggattcc cttttttgta atgcgaggta
    10441 tttgctgaag aaattaataa ttctttttgt aagtcagtaa gtttatccca aaaaggcaag
    10501 ctatgtgtaa gtatatcaat ttcattttgt gaaatcatac aatatctcct ttcttaaatt
    10561 gttataatcc catgatacaa gtgaatagat aaattgacaa gtagtttgtt aagaggatta
    10621 tttagtcaga tagtaatagt atgatttatg tagaaaaaat atgataatat atattcttgc
    10681 tattaatgag aaagtaatat gttttgatga tagtaggttt gtataactaa tgcataccgt
    10741 ttatttttaa aagattaagt tccttatgta accttataga cataaaacca gcaattatag
    10801 cagccatcaa atcagctatt ggttgtgcat ataagatacc atttaaccca tagaatacag
    10861 gaataatcaa aattataggg ataaaacaga caccttgtct acacatacct aaaaatccac
    10921 cttcctttgc ttttcctagt gcgaggaata atgtagaata cactgtatag aatccaaata
    10981 gcataaatga aaacccattt gccctaagag ctttactacc attttctatt aataaaatgt
    11041 caccacttgt aaattgtgaa attatagatt ttgggaagag tatcaatata agaccaagca
    11101 ctacacaaaa tactgtagtc cataatatgg aagtttttat tgctatatgt aatctatcgt
    11161 agttctttga accaaaatta taaccagcaa ttggttggaa accttttata aaaccaaata
    11221 caacaagact acctaaagac gttattcgtg ttacagcacc cattccagct atcacagaat
    11281 caccatactt tgtagcctcc atattagtta atgtaatagc aagactagtc aataattgaa
    11341 atattaaggt aggtatgcca atttttagga tttctgaaaa aatttctttt gaaaaacagc
    11401 attcgttaat tctaaaacta aatatacttt tcttgcttaa aatatatcct acataaacaa
    11461 gtgttgaagc aatctgtgag atagctgttg caatagaagc accccttacc cccagaccaa
    11521 aagtataaat gaatatcggg tctaaaatca tattaagcaa agcacctaat aacatagcac
    11581 acatagttgt tttagcagct ccttcacttg tgacaatatt agtcatagtg acattgaata
    11641 tattaaaaat agatgacacg acataaatgc tagcataaga tattgcataa ggcataatac
    11701 tttttgtagc tcctaacaat tttaaaattg gattgagaaa aatcataatc acaataataa
    11761 ctaatgtacc cactataata ctactgtaaa tagatgtact taccactttt cctgcttttt
    11821 cattatcacc atggcctaaa agtctggcaa catatgaagc agctccattt ccaaacaata
    11881 atcctaatcc cacaacaagc tgacctaatg gaaatgcaac ggatatagct cccatttgac
    11941 ttgttccaag tccacccaca aagtatgcat caaccaaatt gtataatgca gttatcatca
    12001 tacctatcat ggtaggtaat cccatagata acaatgcttt tgagattggg gcagacccta
    12061 ataattcaat tcttttgtta ctttctttca tacttaacac tcctcttcta ttgacattaa
    12121 gtctaaatta taatactata aattatagta agtatggaaa atagtactat aatttatagt
    12181 atttgtcaat agtaagaaaa ctaaaggaga tgatttttat ggctacaata gatttaattg
    12241 tattgggaat agtaaagcaa caacccaaga gtgcttatga tattcaaaag gcagtggaat
    12301 acaggaatat atcacgttgg gttaaaataa gtacaccatc aatttataaa aaagtaattc
    12361 aattagaaca aaagggatat attgaaagta aaactgtgaa gaatggaaaa atgccagaaa
    12421 aagcagttta ttcattaact gaattaggtg agacacattt tttagattta atgtttgata
    12481 catctagcaa accaataaat attcttttag attttaatgc tgttatagta aatttaaata
    12541 gccttacacc agaaaaacag aaattatgtc tcaataatat tgaagagaat gttcaagagt
    12601 taaagaaaac tattgaggaa aatataagta taaagacaga agtaccagac acagcaaaag
    12661 cagtgttgga gcaacagttg gtgctagcac aagcattgga aacctggata aattctttaa
    12721 aagataatat gggaaattag aattagagaa gctaacttaa taatcattaa acaaaaacca
    12781 gcatatttaa taatgagata ttagaaaaaa taaaacaaat gatataaact gaaaagtata
    12841 tttaaaataa agctcactta attgagggag aatgttaaac tttt
//
LOCUS       DAGSWI010000117        12838 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.117, whole genome shotgun sequence.
ACCESSION   DAGSWI010000117 DAGSWI010000000
VERSION     DAGSWI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.117"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..600)
                     /locus_tag="MJF80_002964"
     CDS             complement(<1..600)
                     /locus_tag="MJF80_002964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281607.1"
                     /translation="MYIEELKQNLNNKIGDIINIYLKIYWNRISNRGEKNLYNIYFNR
                     EIKRVLYNKYLENINIDKRDISVVYDCIINDLNSEIDKRIRNSNINELMAILYGIACF
                     QAEFHGRVFITLNNNTSNFSNILHIEKDILRYIERNLKKRFIVDSISATDKSSIQYLI
                     EKCMHFSSDSDNQNNFGVKLFTDVYMMSLIITNLIAEKNM"
     gene            complement(687..1814)
                     /locus_tag="MJF80_002965"
     CDS             complement(687..1814)
                     /locus_tag="MJF80_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBZ0281608.1"
                     /translation="MTIPKLEPSDRKREFDKYTSQQINKVVYNYLFEKNMSNRKIEGE
                     ILNLSSKQNKGYQAMSILHHLGINKNHKGVFMGYTIDKAIEVLKEDEIYFKQIINILL
                     ESKNINKYPIIVDSWELLDEKIALKTIDKSTLKHNRTGVPIEIRHFFEINNLGKNEKK
                     NVNFRYDDNYYSAVIEMDKLENPRSRIIWKSKFRDLLRNIFPKYYEKFLEDEIEGKTN
                     LPKIRFVKFDSNNFDIEFIFTSEIEDDVLEEISQDNPLDIDTINIELETEIRKEGKVK
                     YVYGKQYERNPKNRIEAIKYHGTKCVVCGFDFEKTYGNRGKGYIEIHHIRPLSSVGKE
                     SNINPKTDLVPICSNCHRMIHRKKDSVLSIDELKQLIKDKK"
     gene            complement(2060..2335)
                     /locus_tag="MJF80_002966"
     CDS             complement(2060..2335)
                     /locus_tag="MJF80_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281609.1"
                     /translation="MYGEIIEIYFDYWINEHMYRDILEKDKYYIESWKEIITTNELLE
                     KSLLEEDRRYVLVELNDLFNSLIEFERELSYKFGFKAGVKFIIELLK"
     gene            complement(2583..3909)
                     /locus_tag="MJF80_002967"
                     /pseudo
     CDS             complement(2583..3909)
                     /locus_tag="MJF80_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
     gene            complement(3922..4665)
                     /locus_tag="MJF80_002968"
     CDS             complement(3922..4665)
                     /locus_tag="MJF80_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281610.1"
                     /translation="MSNLGWIKLHRKIKESPLYKDCNSKQRDILINLLLSVNHKPNKW
                     VYGGKEYEVKSGQLITSLESIANMCSKDVSIQNVRTALKKFERYGFLTNKSTNKNRLI
                     TIENWELYQSNEENQQTNQHAINKQLTTNKNINNEKSKIYSEIIEKFNITCTELSKVK
                     KLTEARKKVINARIKEYDKETVLEIIHIVSKTSFLNGSNEKGWKASFDWIMSPNNFLK
                     ILEGNYQDKSINVKVGADAYSPDDSQLKW"
     gene            complement(4686..5000)
                     /locus_tag="MJF80_002969"
     CDS             complement(4686..5000)
                     /locus_tag="MJF80_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281611.1"
                     /translation="MADTNNLEVLKTLQATLNILVKQEQEAQEKAKGYKEIQTYRRDK
                     AQLITCKEASKIYPIGESKFRQLCHSKNKGFPCIWIDNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            complement(5004..5198)
                     /locus_tag="MJF80_002970"
     CDS             complement(5004..5198)
                     /locus_tag="MJF80_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281612.1"
                     /translation="MKLKLKRIEKGIKQYELAQKVGISRYYLSSLERGKSKNPSNMLM
                     LKLAQELDTSVEELFFDKGE"
     gene            5340..6200
                     /locus_tag="MJF80_002971"
     CDS             5340..6200
                     /locus_tag="MJF80_002971"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281613.1"
                     /translation="MINNTIGEKIKQYRKSLNMSRDELANKIGCSVHAIAKYEQNQRK
                     PNLDIIKKISTALSIPINRLIIEENDLDISTNNYTNLKEFDSVVKTFDNYSAKLIGNN
                     ISKFRNINNISIKELSEKINCSENEIINYENGTKMPRIEILKKIALATYTNIEDLFGF
                     PLEEIKIDNQTILYRNPQTGYIINDFLIDFINKDIDIDNTPVSHYIDKSEFDLVIHIL
                     ENYGYDINTDYKTKFEITDNVDTIKPINLNFSDFKNFSKNLNWVINGLVENFIQENSN
                     GLIENEDEEY"
     gene            6289..7041
                     /locus_tag="MJF80_002972"
     CDS             6289..7041
                     /locus_tag="MJF80_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBZ0281614.1"
                     /translation="MNKSYIPKYTYKFPTNLPHGDKKYTLTYIRRMISEFVYDMGKLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNSN"
     gene            7031..7661
                     /locus_tag="MJF80_002973"
                     /pseudo
     CDS             7031..7661
                     /locus_tag="MJF80_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7777..8007)
                     /locus_tag="MJF80_002974"
     CDS             complement(7777..8007)
                     /locus_tag="MJF80_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281615.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVADMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(8013..8492)
                     /locus_tag="MJF80_002975"
     CDS             complement(8013..8492)
                     /locus_tag="MJF80_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBZ0281616.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFNSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            complement(8492..8707)
                     /locus_tag="MJF80_002976"
     CDS             complement(8492..8707)
                     /locus_tag="MJF80_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HBZ0281617.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            complement(9045..10184)
                     /locus_tag="MJF80_002977"
     CDS             complement(9045..10184)
                     /locus_tag="MJF80_002977"
                     /inference="COORDINATES: protein motif:HMM:NF038233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron St85 family RNA-directed DNA polymerase"
                     /protein_id="HBZ0281618.1"
                     /translation="MHLKYSNLMTLESLNLPKIYNLESFSTEIGLSTTILYLLSQKQD
                     KFYHQVIIPKTKSGNRTLSIPSISMKIVQKWIKVKILDNIPVSNCAMAFRKGTNYGIK
                     MNAELHKYNTYILKLDLKDFFNNIEREKVFYLFREIGYNNYISNILSNICTFNEVLPQ
                     GGVTSPSISNLICKRLDRRLENLASKRDITYTRYADDLIFSCNDEVLLIKIKNVILDI
                     LKSEGMEINKLKTKFIRPGSRKKITGLHIDKNKVVVPRDTKRRVRAMIHYAITKEDYS
                     KLDEIKGYISFISSIEGNYISKIKSYINKIVNNGQCRLFGPIVESYNKNKIFKNLELV
                     SCIEEMDWVITKGAFYNREKYELKEVIEKSTEYGEDNINGETSPF"
     gene            complement(10371..11252)
                     /locus_tag="MJF80_002978"
     CDS             complement(10371..11252)
                     /locus_tag="MJF80_002978"
                     /inference="COORDINATES: protein motif:HMM:NF038232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron St85 family effector protein"
                     /protein_id="HBZ0281619.1"
                     /translation="MKIKNSYLNSVNKIYTDIYKNINKEYIDVFLCGGVSTNKSCIRD
                     MVRIELENNKIRVLYPEDLFMDILSKNKSMDLLSLENFLADNSDVICIIPESPGSLVE
                     LGAFTNNKRTLENLFVVINEKYKTQKSFITTGPIKYIMNEKGSNRITYYNEDLNALIT
                     LLKFKFRKFIRMSKNKTMDVDTIIGQYYFMQLVIYFTKHISNKDLKKLINYIYTENKY
                     EKEKFNMIFSSSLKLLYKNRHIKRDIISGKISLTDKGNIYVEGMIYNLNIKNRGKLYD
                     SIRYGILYSELYGKIPL"
     gene            complement(11597..11946)
                     /gene="ssrA"
                     /locus_tag="MJF80_002979"
     tmRNA           complement(11597..11946)
                     /gene="ssrA"
                     /locus_tag="MJF80_002979"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12104..12559)
                     /gene="smpB"
                     /locus_tag="MJF80_002980"
     CDS             complement(12104..12559)
                     /gene="smpB"
                     /locus_tag="MJF80_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBZ0281620.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         4396 a         2035 c         1227 g         5180 t
ORIGIN      
        1 catatttttt tctgctatta aatttgtaat tattaggctc atcatataaa catcagtaaa
       61 taattttaca ccaaaattat tttgattatc actgtctgat gaaaaatgca tgcatttttc
      121 aattaaatat tgaattgaac ttttatcagt tgcacttata gaatcaacta taaatctttt
      181 ttttaaattt ctttcaatat atcttaatat atctttttct atatgtaata tattactaaa
      241 gttgcttgta ttattattca atgttataaa tactctacca tgaaattctg cttgaaaaca
      301 tgctattcca tatagaatgg ccataagctc atttatatta ctattcctaa ttctcttatc
      361 tatctctgaa tttaagtcat ttattataca atcatagact acactaatat ctcttttatc
      421 tatattaata ttttctaaat atttattata taataccctc tttatctctc tattaaagta
      481 tatattatat aaattttttt ctcctctatt agatattcta ttccaataaa tcttcaaata
      541 tatgttaata atatctccta ttttattatt taaattttgt ttcaactctt ctatatacat
      601 tgtagtataa ccccacttag gctttagatt tttttcatta tattacaaat ttcaatctat
      661 tatataagtt caaaaagtat caaatattat ttcttatctt ttataagttg cttcaactca
      721 tctatactta aaacactatc ttttttccta tgaatcattc tatgacaatt tgaacatatt
      781 ggcactaaat ctgttttagg atttatatta ctttcttttc caacagaact taatggtcta
      841 atatgatgaa tttctatgta tcccttacct ctgtttccat atgttttttc gaaatcaaac
      901 ccacatacaa cacactttgt accatgatat tttattgcct ctattctatt cttaggatta
      961 cgctcatatt gttttccata aacatattta acttttcctt cttttctaat ttcagtttct
     1021 aattctatat taatagtatc aatgtctaaa ggattatctt gacttatttc ctctaataca
     1081 tcatcttcta tttcagaagt aaaaataaat tctatatcaa aattattact atcaaatttt
     1141 acaaatctta ttttaggcaa atttgttttt ccttcaattt catcttctaa gaatttctca
     1201 taatacttag gaaatatatt tcttaataaa tctctaaact tagatttcca aattattcta
     1261 cttcttggat tctctaattt atccatctct ataactgcac tataataatt atcatcatat
     1321 ctaaaattca catttttctt ttcattcttc cctaaattat ttatctcaaa aaaatgtctt
     1381 atttctattg gaacccctgt cctgttgtgt tttaatgtag atttatcaat tgtttttaaa
     1441 gctattttct catctaataa ctcccaagaa tcaactatta taggatactt atttatattt
     1501 ttactttcta aaagtatatt aatgatttgt ttaaaatata tctcatcttc tttaagcact
     1561 tcaattgctt tatcaattgt atatcccata aaaacccctt tatgattttt atttattcct
     1621 aaatgatgca atatactcat cgcttgataa cctttatttt gttttgatga taaattcagt
     1681 atctctccct ctatctttct attactcata tttttttcaa ataaataatt ataaactact
     1741 ttatttattt gctgtgatgt atatttatca aactctcttt ttctatcact aggctctaac
     1801 ttaggtattg tcatattatc ctcctcaatt gattatttta gattttaata aaattattgt
     1861 atcatatatt tattattaaa atatgttatg ttatttattt aaatataaaa ataatacaat
     1921 gtaattgatt ccacaaaaaa ttaaagaggg catccttaat accctcttta taaaatatat
     1981 ttaattcaag gtgttatagt acaatttatt cataatctca atctacaatt taattaatct
     2041 actgaaacat acaaaataat tacttcaaca gctcaattat aaattttact cctgctttaa
     2101 atccgaattt ataactaagt tctctttcaa attctattaa tgaattaaat aaatcattta
     2161 gttccaccaa tacatatctt ctatcttctt ctaataaact cttctctaac aattcattag
     2221 ttgttataat ttctttccaa ctttcaatat agtatttgtc tttttctaaa atatctctat
     2281 acatatgttc atttatccaa taatcaaaat atatttctat aatttcacca tacatgttag
     2341 tacctccttg actttcatct ggcacttaat atatacttaa cttgtgtagg ttagtgtaca
     2401 ttaagtacct aatttataaa ggtagttgtt atatttatat ggcaactacc tttttctaat
     2461 aaatttttca atctaaatac tatatatttt aaatctttat tgcatacaat taaggattat
     2521 atacttttct tctaacactc cttaaacaat agtttatata tcacttaata aattatattt
     2581 tttcatacct tcttacattc cttaaaataa aacaaacttc ctatatacca ataaggtcta
     2641 aagcctgatt ccccatctct atttttagca actataattt cacttaaatt agctccaaaa
     2701 cctttttctc tacgtcctaa ttcttttata tcctctattc ccattatctt acaataatca
     2761 agagcctctt tctctgtagg tctatgaata tatataactg aattagaatc catatatatc
     2821 tgttttgaac ttctcattac tctatcacct tttggtcttt catccttcaa ttcatcattc
     2881 aattgagcta actgtataat tggaatatta aaatctaaag taatcttttt tatttgcctt
     2941 gataaatcag caaatttagc ttctccaact tctttacctt cgtaatccaa aatctgaaga
     3001 taatcaatta taacaacatc tggtttaata ctttttattt ctctcttaat tcctgatatt
     3061 gtattgactg aatcattaat atagagatta ttttcctgat ttaaagtact taaagcattt
     3121 atgtatttaa ttattttctt cttcatttag atttctattc ttcattttta caactgatat
     3181 tccagttaaa gaagctatgt ttctaaacaa aatttgtata tcactcattt cctcacttat
     3241 aaaaagtact tttttacctt gaaataccac atttctcatt atctgtaagg atagtgctgt
     3301 ttttcctctt ccacttttgg ccgctattgt aattaattca ccactatata gacctccaat
     3361 agtttcatct aaaaatttta ttcctatatt aataccttct tcaatttcat catttatata
     3421 ttcaaaggct ctttctgata tagctgttat actagaatcc ttcttaacca atttctgatt
     3481 gacttcatta atgtattttt ctaattgata tataagtgta ttatcatcta tttcccctaa
     3541 tttgaattta ttaaatttat caattatatt tctattttta tatttttcaa taactatatc
     3601 aatataatga gatacattac ctatagttgg aactattgtt gatagttttg tcaaataact
     3661 tatacctcca acataatcta taagcccctt tttctttaaa tcttctgtaa gagttattat
     3721 gtctataggc tcatcccttt tatgtatatc tttcactgaa ttgaatatat gtttgtgttc
     3781 ttgatgataa aacatatctt cacttattaa tatttcaatc tcttctattt tcctcgaatc
     3841 tagtaaaata ctacctaata tagattgttc tgcttctgtg ttcttaacta aattactcat
     3901 attatccata atataaactc cctaccactt taattgtgaa tcatctggac tatatgcatc
     3961 tgcccctact ttgacattaa ttgatttatc ttgataattg ccttctaata tcttaagaaa
     4021 attatttgga ctcattatcc aatcaaaact tgccttccaa cctttttcat tgcttccatt
     4081 taaaaagcta gttttactaa ctatatgaat aatttctaat acagtctctt tatcatattc
     4141 ttttatccta gcattaatta ctttttttct agcttctgta agtttcttta cttttgatag
     4201 ttctgtacaa gtaatattga atttttctat aatttcactg tatattttac ttttttcatt
     4261 atttatattc ttgttagttg ttagttgctt gttaattgca tgttgattag tctgttgatt
     4321 ttcttcatta gattgatata gctcccagtt ttcaatagtt ataagcctat ttttgtttgt
     4381 tgatttattt gttagaaatc catatctttc aaatttcttt aaagcagttc ttacattctg
     4441 aatacttaca tcttttgaac acatatttgc tatactttcc aaagatgtta ttaactgtcc
     4501 agatttaact tcatattctt tacctccata aacccattta ttaggcttat gattaactga
     4561 cagtaatagg tttatgagta tatctctttg tttggaatta caatctttat atagtgggct
     4621 ttcttttatc tttctatgaa gctttatcca acctagatta ctcaaatact atcacccact
     4681 taagcctata attttatgcc atttgcattt tcaataaacc aatcatctag tttatttttt
     4741 attatataca ctctattatc tatccatata caaggaaatc ctttattttt agaatgacat
     4801 aactgtctaa attttgattc ccctatggga taaattttag aagcttcttt acaagtgata
     4861 agttgagctt tatctcttct atatgtctgt atctccttgt acccttttgc cttttcttgt
     4921 gcttcttgtt cttgtttgac aagtatattc aatgtagctt gtagtgtttt aagaacttct
     4981 agattatttg tatctgccat agatcattcc cccttatcaa aaaataattc ttctacactt
     5041 gtatctagtt cttgagccaa ttttaacatc aacatattac ttggattttt ggatttgcct
     5101 ctttctaaag aagataaata atatcttgaa atgccaactt tctgagctaa ttcatattgc
     5161 tttatacctt tttcaattct ttttaattta agcttcattg atttcctcct tcctcacata
     5221 cccatattgt gcctttttat ctttctacaa aacagaataa cacccatttc gtgctttatc
     5281 aatatattta agtgtattaa tacacccatt tcgtgctata atgtatttga ggtgaaatta
     5341 tgataaataa tacaattgga gaaaagatta aacaatatag aaaatcatta aatatgtcaa
     5401 gagatgaact agctaacaaa attggttgct cagttcatgc tatagctaaa tatgaacaaa
     5461 accaaagaaa acctaattta gacattataa aaaaaatatc tactgcttta tcaattccca
     5521 taaatagact tattattgaa gaaaatgatt tagatatttc aacaaataat tatactaatt
     5581 taaaagaatt tgattcagtt gtaaaaacat ttgataatta ctcagcaaaa ctcatcggaa
     5641 ataatataag caaatttaga aacataaata atatctctat taaagagctc tcagaaaaaa
     5701 taaattgctc tgagaatgaa attataaatt atgaaaatgg aacaaaaatg cctagaattg
     5761 aaatattaaa aaaaatagca cttgctactt ataccaatat tgaagattta tttggattcc
     5821 cattagaaga gataaaaata gataaccaaa caatccttta tagaaaccct cagactggat
     5881 atataataaa tgactttctt attgatttta taaataaaga tattgacata gataatactc
     5941 cagtttctca ctatatagac aagtctgagt ttgatttagt gattcatata ttagaaaatt
     6001 atggttatga tataaataca gactataaaa caaagtttga aattacagat aatgtagata
     6061 caataaaacc aattaaccta aacttctctg atttcaaaaa cttctctaaa aatctaaatt
     6121 gggtgataaa tggtcttgtt gaaaatttta tacaagaaaa ttctaatggt cttattgaaa
     6181 atgaagatga ggaatattag tactatgcat tttcacaata taaatttaat tgttaatttt
     6241 gatatagtca taattagata agaattaaat aaggaaggag ctacaatcat gaataaatca
     6301 tatataccaa aatacacata taaattccct actaatctac ctcatggaga taaaaaatat
     6361 actttaacat acattagaag aatgataagt gaatttgtat atgatatggg taaactagaa
     6421 aataacccct ttacattccc agaagtacaa acattacttg atggtataac agtaggtgga
     6481 cataaattaa gtgatcaaaa tcaaatacta aatataaaat catcatggga ttatgtaata
     6541 aaattaagta acaaagaaaa cttatcttta aataaagata caatttgctc tatacataaa
     6601 atagttgcaa aagatgaagc tctaatcgtt ggagatttca gaaatggtaa tataggtata
     6661 gctggtacta ctcaatatga atgcatagaa gccactctat taaatgattt atttgtaaat
     6721 gacataaata tcataaataa aataactaac ccattagaaa aggctataat aataaattta
     6781 tggttgtcct attgccaatt cttctatgat ggcaacaaaa gaactgctag acttacctca
     6841 aacctaatac ttataagtaa tgatatagga gttctatcaa taccagctaa acataaacaa
     6901 gaatacaaca cattaatgct aaacttctat gaaacactag aagctgatga agttataaaa
     6961 ttcttattag aaaaatgtat tacatttttt gatggatata actataaatc atataaggag
     7021 ctgtttaaaa atagcaacta aaagagctaa tggagaatgc tctatcggaa aatataaaga
     7081 tggttggcgt tcccgtataa tgattggcta taatgaagat ggtaaaccaa taagaaaaga
     7141 attttatggt aagactcaaa aggaagttaa agataaactt gatgcttata aaaaacaata
     7201 ttatttaagc tctgatattt cagatgataa aataacttta gagcattggt tttacaattg
     7261 gttatttgaa tatagaataa aagatttaaa acctaagtca tttgaaagat atgaaagcat
     7321 ctatcataat tatattaaag atactgattt aggtaatgtt aaattaaagg acttaagagt
     7381 atctcacata caaaaatatt acaatagatt gcttgattcc aataaaagta tacctactat
     7441 aaggcaaata aacactaaac tgaaaacctg cttatcagaa gctgaaaaac aaggtcttat
     7501 acaacgtaat tattgtacta tggtaaatat accagttgag aaaactgtac aagttttaat
     7561 gggtcattat gatatatcaa taactttaaa tatatactca agttatggaa gacaagaaag
     7621 ttgaagctgt agaaaagttg gataaaatat tttcattgta aaaatcaaat acatataaat
     7681 attgtaattt catatagtta attacaaaat caaaataagg atacaaatta attttatatt
     7741 atcttgtatc cttatttcgt atctagtttt aattatttat cttggatagc ctttatgata
     7801 cattaatcca ttttctaaca ttaattcttg agccaagtat atatgaccct tacagtgcat
     7861 atctgccaca ccttcactta taagtttata tgtttcagaa gtatataaat aatccatact
     7921 ttcttccaag ctcttacctg tttgcttaga aaattctaat atgatttttt tatatagtat
     7981 ttgtaataat gttttatttg cttccataat cactatacct cctcttcata cctaaacttt
     8041 aaatatttat ctattacatt ttgattggtt atacatattt gttcatttgg tttatagtat
     8101 tttaatcttt ttaaagcttc atctttttca ataagattat cctgatacag ttctattgta
     8161 ttgtaaatct tatcatcagc aataccacct attataatgt catatttcat atatatatca
     8221 ctcatatttc tacattctat tataaaatca agccattcta aattataact attaaagatt
     8281 tttaccttgc agttttcttt taatttatca atatctaaag aataactatt taaatatgct
     8341 tttttacctc tttttttaaa acgctctgtc cagttaattg cttgtgtctt tatactggtt
     8401 acatagaatc cttttccaaa atctaaagaa tctcttgaaa aggtaatact aggagtcttt
     8461 accacacaat atgaaccatg atatacaatc attatatcac acccttttct ttaagtactt
     8521 gcattaaatc ttccactatg taacctttac tttgagaatg tagtgcttca tagcaaggta
     8581 ttatatagtt atctaaaata tcagtatcat gtttaagaag tttataaact ccttctgaat
     8641 ctatattcaa tttttctgct aaagcttcaa tgcaaaaaat agaaaattct agttttcttt
     8701 catccattac aaacctcaac tcctatcaag cttatataat atatattttt actaattaac
     8761 aacttaaact gtcttacatt tataaaaata ttatatcata aagtagatat taaaattctg
     8821 cattagttaa tctctaataa acaaataaag actttttata caataaaatc cctcccaatg
     8881 ttctttcaaa aaagctcttt ttttgtaatc gaaatacaat ttaatggatt tcatcacata
     8941 aataaaaaat actagccaat aaagactagc attttcaatt attttatcat tcatttttca
     9001 aaatagataa atactattta caaaactccc acaatcactt ttatttaaaa tggacttgtt
     9061 tcaccattta tattgtcttc accatattca gttgattttt ctataacttc tttcaattcg
     9121 tatttttctc tattataaaa agcacccttt gttattaccc aatccatttc ttcaatacaa
     9181 cttactaatt ctaaattttt gaaaatttta tttttattat atgattcaac aataggacca
     9241 aataacctac attgtccatt attaacaatt ttatttatgt aagattttat ctttgatata
     9301 taatttccct ctatagatga aataaaagat atataccctt ttatttcatc taactttgaa
     9361 taatcctctt tagttattgc ataatgtatc attgctctga ctcttcgctt agtatctctt
     9421 ggcactacga ccttattctt atctatatgt aagcctgtaa ttttttttct tgaacctggt
     9481 ctaataaact ttgtctttaa tttatttatt tccatacctt cagatttcaa tatatcaagt
     9541 attacatttt taattttaat taataaaact tcatcattac atgaaaatat taaatcatca
     9601 gcatatcttg tataagtaat atctcttttg cttgctaaat tttctaacct tctatctaat
     9661 ctcttacaaa ttaagttaga tatagaaggt gatgttactc caccctgagg caatacttca
     9721 ttaaaagtgc atatattact taatatattt gagatgtaat tattatatcc aatctctcta
     9781 aacaaataaa ataccttttc tctctctata ttgttaaaaa aatctttcaa atctaattta
     9841 agtatatatg tattatactt atgcaattct gcattcattt taataccata attagttcct
     9901 tttctaaaag ccatagcaca atttgaaact ggtatattat ctaaaatttt tacttttatc
     9961 catttttgaa ctattttcat tgatatactt ggtatactca atgttcgatt gcctgattta
    10021 gtttttggta ttattacttg atgataaaat ttatcttgtt tttgagataa taaataaaga
    10081 atggtagtac taagcccaat ctcagtacta aatgattcta gattatatat tttaggcaaa
    10141 tttaatgatt ctaatgtcat taaattacta tattttaaat gcataatgac aagtacctcc
    10201 ttaccataaa tcttttgagt taattgtttc aatttttcat acttaatgtt gaataaattg
    10261 aaacaattac gaaatataaa tacacttaaa ttctaacaga gacgaatcat ctctaactat
    10321 aataagtacc atataacatg cgcatctata gcaacttata acagtgttct ctaaagaggt
    10381 atcttgccat ataattctga atatagtata ccatacctta tagaatcata caactttcct
    10441 ctatttttta tatttaaatt gtatatcatt ccctcaacat atatatttcc tttatcagtt
    10501 aaagatattt taccacttat aatatctctc ttgatatgtc tatttttata taataatttt
    10561 aaagaagaac taaatatcat attaaatttt tccttctcat atttattttc agtatatata
    10621 tagttaatta attttttcaa gtctttatta gatatatgct ttgtaaaata tatcactaat
    10681 tgcataaaat aatattgtcc tattatagta tccacatcca tagttttatt ttttgacatt
    10741 cttataaact ttctaaattt aaattttaaa agtgttatca gtgcatttaa atcttcatta
    10801 taatatgtaa ttctattact tcctttttcg ttcataatat actttattgg tccagtcgtt
    10861 ataaaacttt tttgtgtttt atatttttca ttaattacta caaataaatt ctccaatgtt
    10921 cttttattat tagtgaatgc tcctagttct actaatgatc ctgggctttc aggaattata
    10981 caaattacat cactattatc agctaagaag ttctctaaac taagtaagtc catagactta
    11041 tttttactaa gtatatccat aaataaatct tcaggatata aaactcgtat tttattattt
    11101 tcaagttcta tcctaaccat atctcttata caacttttat tagtacttac tcccccacat
    11161 aagaatacat ctatatattc tttattaata tttttataaa tatctgtata tattttatta
    11221 actgaattca agtaactatt ttttattttc acttaatcct ccatattatt tagtttattc
    11281 ttattctact aattatacta tttttaaaca aatatatttt agatacgtat aggtagcaat
    11341 aatctatata ctattaatta tatcaattat ttagaagatt taaaatcaat gtcttatctt
    11401 aattctatta tttatatgta taatactatt tttagtatat ttataattta atgtataatt
    11461 atacacttaa atgcacccca atataccccc aaaaaaacct ctttttctgt ggttggggta
    11521 cggttggtgg attttactcc aaaataaaaa atactagtca ataaagacta gtatttcatc
    11581 attctttttt caaaattggt ggagacgagg ggagtcgaac ccctgtccgc aaatcttttc
    11641 cttgctgcat ctacatgctt agccaacttc actaatatct cacctcacat agcgccaagt
    11701 ggcaggctct atgcttagcc agtcccaatt aatctacttc aagtcgagac acccctaaag
    11761 tagttccctg cataatgacg tccagtctta gtcggcagga ggactaagag ggacgagcag
    11821 caactaggct gctatcgcga aattatcgtc tgcgtttatt ttaaagttcc ccagttttta
    11881 cgtgacccag agtaacacga catgcagcct caagttccaa acccacgtcg aaacctttac
    11941 gcccccttga gtagtttata gtccagtaga cttttcgcaa actttatcac catttttatt
    12001 aaatttttaa tgtatatata atataatact tttaagaata aaaatcaatg ttatgttcta
    12061 gataataaag aattttattc taaaaaatac agttttatct agtttaatac ttacctgtca
    12121 tttctctttc tatacgtctt tgtgcatctt tcttagccat atcttgacgt ttatcatata
    12181 gttttttacc ttttgctaag gcaatctcta ctttaacttt accgtttttt agatacaaac
    12241 taagtggtat aagtgaatac ccctttatag tagttgctcc aataagtttt cttatttcat
    12301 gcttatgaag taataatttt ctaactctta aagaatcaac attgaatata tttccttgct
    12361 catatggact tatatgaact tgttttaaaa atacttcgct attatctaca gatgcaaatc
    12421 catcacttag gttaactttg ccttgtctta ttgattttac ttctgttcct tttaattcaa
    12481 ttccacattc ataagtttct tctataaaat attcatgtct tgctcttttg tttgttgcta
    12541 atattttttt accagccata taaatctcac cttctttgct agaaatttgt tccataaatg
    12601 ttagaaattt actaaatatt taatgttgcc aaattatttg attaacttgt atgataacat
    12661 atttaggcat tttagtcaat atttcactga tatattaagt tagttaggct atatataatt
    12721 aagtaatata aatgtatgaa aatagctaaa aaactacttc gaatatactt aatactaaac
    12781 taactctaac cagtaatact aatttataaa tttcaaaaca aaaatattac atattata
//
LOCUS       DAGSWI010000118        12805 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.118, whole genome shotgun sequence.
ACCESSION   DAGSWI010000118 DAGSWI010000000
VERSION     DAGSWI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.118"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(44..4147)
                     /locus_tag="MJF80_002981"
     CDS             complement(44..4147)
                     /locus_tag="MJF80_002981"
                     /inference="COORDINATES: protein motif:HMM:NF016121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBZ0281621.1"
                     /translation="MRKKSKIIAGGILITSVIGATSFAIIDPYNILSNKNMKIPEQSR
                     KLEKSNNKEKEDIDNKKDENNMNLNGIKDGTYLGEAKGYGGNIKVKVIIESGKIKNIE
                     VLSHSETPKYYENGSKVIGNIIRANSTDVDAVSGATLTSNGIKNAVRDAISKAGFNVS
                     KDNKEVSVASNSAKSRPVSSNNMVKNIDLKEYKIKDGEYIGEAIGFKGNVKVKVIISG
                     GKLSDVKVISHNDDAEFFNKAKSVIIKILRNQGTAGVDTVSGATYSSRGIINAVNSAL
                     NKVAKESNGIFNTIKIFENDNIPRKDSNPKFNVKDIVKEALHQKSISIDKDDNISSRE
                     HKFKDGEYIGEANGFKGNVKVKVIIKNGTLVNIEIINHNDDKEFFNNAKRLIFKILKN
                     QGTTGVDTVSGATYSSKGIINSVNRALNKAVNKNITTQDTIVQSKGDKVKENVDIKDI
                     ENPNDKFTQKENQEISKDNKEKESSKSVKYLDGSYKVSGIGFTGKPIKSVVTFENNKI
                     KSIDIGTIESGDFGDNEPFRPIAIKVVDHILSDNGGKSINDLILHGEIVDKIFKSNNY
                     YEVGKNLIGDYAGELKGIYVSGRIAHEKISSVVKKYMKAKNSSTVLDSVSGATFSAIG
                     IAKSVKDAMNKSANDYETDNIVNELKIKTPSEKRIYVDLVYSTKDKKLDLSNLKVIIS
                     MRDGKEKEISYDKFKENDIEIHDKETGKAITNGMDLSSYESGHGIYATIKHKKSLSTD
                     TLLIIPRFFDKMDTNHLTRMEYSIDNGTSWKALSSLEMNEKNINFYQTIKLPKEFKGK
                     DISLRVVSTNKNTYLLKPEKSGQSLKVEKEKYDLITNEEDRLSNHNLRDRYRIVFEFI
                     ENSGNVNKEIELNNKDIKYLDGKYIGIGKGWTSDPIKSKVIFENNKIKNITIEDFPDD
                     IECRDKAEKVIPLLKENPENIINDMLLLDEIAGKIVSSDNYYEKGKELIGNYAENLNG
                     FEKNTFVTVAKYLREQKKAAVFDVVSGATYSAKGIVRSVKDAMDKSANDYKTGNIINN
                     LKIKSPSNKNMRVDKKEKLDLSNLKIVISMRDGKEKEIGYNELKENGIEIYEEETKKT
                     IYNGMDLSSYERNHAICAVIEHKNSLNKDKLIIIPEIIDKNYITGMEYSVDNGVTWKN
                     ISGLKKMNNSNNIHFNQELKISKEDERKVLLRAVSINGDKYGLNLKEKIEGPNGYCIF
                     EPSKDDKAKNKHVANGFNISFKFTNTTDNNVATSLEKVSYARIYLNDSVIYKQYQGKL
                     KVGQKIDWTGAKFKFKNSEQESIKDLFTEDEIKNLKNSNSYKKEGYFNYEDLEKLGIT
                     ISPNQNSEVTTDLNVTLTYKGKTFKVKGGGINGDDDFVGIILEKQEK"
     gene            complement(4160..4555)
                     /locus_tag="MJF80_002982"
     CDS             complement(4160..4555)
                     /locus_tag="MJF80_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBZ0281622.1"
                     /translation="MNKKVIFSIGIVSLGVALSGCSEKGMDIKDNKSERVSITANSAT
                     FKDGTFEGEGEGFENGKTKVSVEVQDGKIKKIDVLNSEDDESYLDEAKKLIPEIIKKQ
                     SSDVDSISGATYSSKGIISAVENALKNAK"
     gene            complement(4846..6183)
                     /locus_tag="MJF80_002983"
     CDS             complement(4846..6183)
                     /locus_tag="MJF80_002983"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281623.1"
                     /translation="MTRMERRLRKIKNLKNKTAMIGVTASLALSTISVVTPNIVVHAE
                     SLSKGAMITNLEDNTYNKIEEITKLFSENNKDKAPVYKEKILKIKSEYLEKMNGIEDP
                     SGIRNYYTEAIKKINEIFREAKKEEVKKITESKKAEEKKTDASKPKTPSNPDTGKTQA
                     KPEEKKPEAESKDIAEDTLNMFKEIKQIYHIYKFKADFNKDIIKKFKDPVEYIYVYEN
                     EMKKLKYEPEKLKEYCKTNPKINELREIHKILKEERKKYDESKKAEEKKTDASKPETP
                     SNPDTGKPQSKPEEKKPEAESKKEERKKYDESKKAEEKKSDVSKTETLSNPDTGKPQV
                     KLEEKKPEVDVKQNEEKPDAKHYNKNTEKELNKDELNKSKPNEISMVKKENNKNVIKD
                     QSKVMSSRESKAETKNIKDVPKTGEASSLPYIGGILISLAAFLKLNMKKFKKN"
     gene            complement(6242..7195)
                     /locus_tag="MJF80_002984"
     CDS             complement(6242..7195)
                     /locus_tag="MJF80_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBZ0281624.1"
                     /translation="MKETLNVRDNSPENKFFMKGFYFDTVVEISICCCQSKGDDILNK
                     SFDLCKYYEELLSKTKKGSDIYNVNNSGVDGVDVHDETLNIIKKSLYYSEISNGNFDI
                     SIQPLLRLWNFKNEEKIIPSQKDIETNLELVNYKNIDVNKNNVRFKISGMSIDLGGIA
                     KGYIANKIKEYLISEGVRSGIINLGGNVLCIGKKDNGEKFKIGIQRPFSERGEVLGIL
                     EVCDKSIVSSGIYERFFEKEDKIYHHIINPKNGRPFESDLVQVTIVSDDSMDGDALST
                     IALSLGEKEGIKLINSIDRVEGLFINKNGTIRVTENFESIL"
     gene            complement(7803..8870)
                     /locus_tag="MJF80_002985"
     CDS             complement(7803..8870)
                     /locus_tag="MJF80_002985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281625.1"
                     /translation="MNKKVKILIASAALSCGVSNFIVPQRVEAKEISTMARESRLESK
                     KSNHEVNNNKSTEKVNEARENAKSKLKAKFEEANKELKKLGDKALGKSFAEINAIYEV
                     GNIEINKAQDTNKVNEALNSATESMDKTLNEALNSAGELERILNELKPNETEKPKHEQ
                     NQMNSTLKEKVKNMFPKLEKIASANYGRYYIQGPISVLKGIVDKNFAGYSDETIKTII
                     DDIQYYFGHSVGAEVGENGVDGFIAAIDKLYSKFENKSKHEQNQMNSTLKEKVKNMFP
                     KLEKIASANYGRYYIHGPISVLKGIVDKNFAGYSDENIKTIIDDIQYYFGHSVGAEVG
                     EDGVDGFIAAIDKLYAEFENR"
     gene            complement(9159..9677)
                     /locus_tag="MJF80_002986"
     CDS             complement(9159..9677)
                     /locus_tag="MJF80_002986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281626.1"
                     /translation="MKVVRNLIIVLFLLLLTGCSSTPATVENHKNIVDPGNDTNKIEN
                     KKDSIASNDGTPIEIKEKMFIAQLDDIFNNSDDYIGRTIKIEGLYNSLEADGKTLNYV
                     YRHSPGCCGNDGIVCIEFVGNDKMTMLNPDDWIEVIGKIEAYKDKTFNSVRIRLETLN
                     VKTQRGAEFVSQ"
     gene            complement(9674..10504)
                     /locus_tag="MJF80_002987"
     CDS             complement(9674..10504)
                     /locus_tag="MJF80_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBZ0281627.1"
                     /translation="MNLVSEMMSYPFIVRAVIVGILVSLCSSLLGVSLVLKKYSMIGE
                     GLSHVSFGAIAIAVALGVSPLKLSIPIVIIVAFLLLRIKESSKIKGDSAIALISSSSL
                     AIGVISIAITTGMNTDVCNYMFGSILAMSKNDVYLSVVLSIVVIFLFTIYYNKIFAIT
                     FDENFTKSTGINVNFYNMMIAILTAITIVLGMRMMGAMLISSLIIFPSIISMKIFKSF
                     KSVVISSGLVSILSFCIGMTISYKFSTPTGASIVVVNIIIFILSIIGEKIKSNNRSYI
                     "
     gene            complement(10500..11210)
                     /locus_tag="MJF80_002988"
     CDS             complement(10500..11210)
                     /locus_tag="MJF80_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281628.1"
                     /translation="MNLVEFNNTSFSYENKLVAKDISFTVSKGDYLCIIGENGSGKST
                     LIKGILGLVKPCKGSIFISEELKDSTIGYIPQQSDIQRNFPATVYEVVLSGCIKRNSV
                     FPFYRKSDKVLAHENMEKMKISDLKNKCYSDLSGGQQQRVLIARALCATDKLIVMDEP
                     ITGLDQKTTLNLYDMLHNLNKNYGLSIIMVSHDIKTSLNYANKVVHMKNRMLFYGDVK
                     DYKQSDCGKKFLGGEIND"
     gene            complement(11213..12241)
                     /locus_tag="MJF80_002989"
     CDS             complement(11213..12241)
                     /locus_tag="MJF80_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBZ0281629.1"
                     /translation="MRKVTVIGMSIILIILSVLTGCTRTESKENDKNNISSRKDKLKI
                     VTTLFPQYDFAKQIAGDRADVKMILSPGQDSHSFEPTPEDIKKIQNADIFIYNGGEAE
                     EWIEKIIDSVDTKKVNAIKMMDYVKAVKEEEVEGMEPEVEHDHDHEHDKEKSHKHDEN
                     DNHNHDEEYDEHIWTSPKNAKNMVEAIKNTLVKVDNTNEKYYNANYENYTKKLDELDK
                     SLTNTVAKAKRKMIVLGDKFPFRYLAKDYGLTYRAAFNGCSTQTEPSIDTVTYLIKTV
                     RENKIPVIYHIEMSNKKIAKTISDETGAKVLLLHSAHNVTKEDFDKGITYIDIMNNNI
                     GNLKEGLQ"
     gene            complement(12248..12670)
                     /locus_tag="MJF80_002990"
     CDS             complement(12248..12670)
                     /locus_tag="MJF80_002990"
                     /inference="COORDINATES: protein motif:HMM:NF013629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBZ0281630.1"
                     /translation="MNNKTVYKTKQKEIILDYLKSNEDSHVTIDEVFNYLSEQGNKVG
                     RTTVYRHMEKLADDGILRKYYIEEGIGSCYQYIGEREDCHQHFHLKCTECKELIHLKC
                     SYLEDLNNHILKDHNFKINNTKTVFYGICENCRDKLER"
BASE COUNT         3777 a         2269 c         1186 g         5573 t
ORIGIN      
        1 atttttaaac cgttcaaaat aatacaaaca ggcatgtagg gttttatttt tcttgttttt
       61 ctaaaattat tccaacaaag tcgtcatctc cattaattcc tcccccttta actttaaatg
      121 ttttaccttt atatgttaat gtaacattaa gatcagttgt tacttctgaa ttttgatttg
      181 gagatattgt tattcctagt ttttcaaggt cttcataatt aaaatatcct tctttcttat
      241 aggagttact attttttaag tttttaattt catcttccgt aaataaatct tttattgatt
      301 cttgttctga atttttaaat ttaaacttag cacctgtcca atcaattttt tgaccaactt
      361 ttaatttacc ttgatattgt ttatatatta cagaatcatt cagatatatt ctagcatagc
      421 ttactttctc taaacttgta gctacattat tatcagtagt gtttgtaaat ttaaagctta
      481 tattaaatcc atttgcaaca tgtttattct ttgctttatc atccttactt ggctcaaaaa
      541 tacaatagcc attaggaccc tctatttttt cttttaaatt taatccatat ttatctccat
      601 tgattgatac tgctctaagc aatactttcc tttcatcttc tttagaaatt ttcaattctt
      661 gattaaaatg aatattatta ctattattca tcttttttaa accagaaata ttcttccaag
      721 taacaccatt atcaacgcta tattccattc cagttatata atttttatca ataatttctg
      781 gaattattat caacttatct ttgttcaaag aattcttatg ctcaatcaca gcacatattg
      841 catgatttct ttcataacta cttaaatcca ttccattgta tatagttttt tttgtttctt
      901 cctcataaat ttctatacca ttttctttta attcattata tcctatttct ttttcttttc
      961 catcacgcat tgatattact atttttaaat ttgataaatc taatttttct tttttatcta
     1021 cacgcatatt cttattactt ggagatttta tctttaaatt atttataata tttcctgttt
     1081 tgtaatcatt agctgactta tccatagcat cttttacaga ccttacgatt cctttagcac
     1141 tatatgtagc tcctgaaacc acatcaaaaa cagctgcttt tttttgttcc cttaaatatt
     1201 ttgccacagt tacaaaggta tttttttcaa atccattaag attttctgca taattaccta
     1261 ttaactcttt ccctttttca taataattat ccgaacttac tattttacca gctatttcat
     1321 caagaagtaa catatcatta ataatatttt cagggttttc ttttaaaaga ggaataactt
     1381 tttcggcttt atctctacac tctatatcat ctggaaaatc ttcaatagtt atatttttta
     1441 ttttattatt ttcaaatatt acttttgact taattggatc acttgtccat ccttttccta
     1501 tcccaatata ttttccatcc aaatacttaa tgtctttatt atttaattcg atttctttat
     1561 taacgttacc agagttttca ataaattcaa aaactattct ataacgatct cgaagattat
     1621 ggttgcttaa tctatcttct tcatttgtta ttaaatcata tttttccttt tctaccttta
     1681 aagattgtcc agacttttct ggctttaaca aatatgtatt tttattagtt gatactactc
     1741 taagtgagat atctttacct ttaaattctt taggtaactt tattgtttga tagaagttga
     1801 tatttttttc attcatttct aaactagata aagccttcca tgaagtcccg ttatctatac
     1861 tatactccat tctagttaaa tgatttgtgt ccattttatc aaaaaatctc ggaattatta
     1921 atagtgtatc tgtggatagt gattttttat gtttaatagt agcatatatc ccatgacccg
     1981 attcataact acttaaatcc attccatttg taatagcttt tcctgtttcc ttatcatgaa
     2041 tttctatatc attttcttta aatttatcat atgatatttc tttttctttt ccatcacgca
     2101 ttgatattat tacttttaag ttggacaaat ctaacttttt atctttagtt gaatacacca
     2161 aatctacata tattcttttt tcactaggtg tctttatctt taattcatta acaatattgt
     2221 ctgtttcata gtcattggct gatttattca tagcatcttt tactgatttt gcaattccta
     2281 tagcactaaa agttgctcct gaaactgaat ctaaaacagt agaactattt ttagctttca
     2341 tatatttttt tacaactgat gatatttttt catgtgcaat acgaccactt acatatattc
     2401 cctttaactc tcctgcataa tcacctatta aattttttcc tacttcataa tagttatttg
     2461 atttaaatat tttatctact atttctccat gtaaaatcag atcattaatt gatttccctc
     2521 cattatcact taatatatga tcaacaactt ttatagcgat aggtctaaaa ggttcattat
     2581 ctccaaaatc tccggactct attgttccaa tatctatact ttttatttta ttattttcaa
     2641 atgttactac agacttaata ggctttcctg tgaatcctat tcctgatact ttgtagcttc
     2701 cgtctaaata cttaacactc ttgcttgatt ctttctcttt attatcttta gatatctctt
     2761 gattttcttt ttgagtaaac ttatcgtttg gattttcaat atctttaata tctacatttt
     2821 cttttacttt atcaccttta gactgtacaa ttgtatcttg agtagtaata tttttattaa
     2881 ctgctttatt taaagctcta ttgactgaat ttattattcc tttgcttgag taagtagctc
     2941 ctgaaaccgt atcaactcca gtagttcctt gattttttaa tattttaaat attaatctct
     3001 tagcattgtt aaaaaattcc ttatcatcat tatgattaat tatttcaatg tttactaatg
     3061 taccattttt tattataact tttaccttta cattaccttt aaagccatta gcctctccta
     3121 tgtattctcc atctttaaat ttatgttctc ttgaacttat attatcatct ttatctatgc
     3181 tgatactttt ttgatgtaat gcctctttaa caatatcttt tacgttaaac tttggattgc
     3241 tatcttttct aggtatatta tcattttcaa aaatctttat tgtattaaaa attccattac
     3301 tttctttagc tactttattt aaagctgaat taacagcatt tattattcct ctacttgaat
     3361 aagtagctcc tgaaactgta tcaactccag cagtcccttg atttcttaat attttaataa
     3421 ttacactttt agccttatta aaaaactctg catcatcatt gtggctaata accttaacat
     3481 ctgataattt accaccactt attatcactt ttacttttac attcccttta aaccctatag
     3541 cttcccctat atattctcca tctttaatct tatattcttt taaatctata tttttgacca
     3601 tattattaga tgataccggt ctacttttag cactattact agctacactt acttctttat
     3661 tatctttact tacattaaac ccagctttag atattgcatc tctaacagca ttttttatcc
     3721 cattagaagt taaagttgct cctgaaacag catctacatc agtagaattt gctcttataa
     3781 tatttccaat aactttactt ccattctcat aatatttcgg tgtttctgaa tgagaaagca
     3841 cttcaatatt tttaattttt ccgctctcta taataacttt taccttaata tttcctccat
     3901 acccctttgc ttctcctaaa tacgttccat cttttatccc atttaaattc atattgtttt
     3961 catctttttt attatcaata tcttcttttt ccttattgtt gctcttttca agttttctac
     4021 tttgctctgg aatcttcata tttttattac taagtatatt ataaggatct attattgcaa
     4081 aactcgttgc accaattaca cttgtaataa gaataccacc agcaattatt tttgactttt
     4141 ttctcataag ttcttcctcc tatttagcat ttttcaatgc attttccaca gctgaaatta
     4201 taccctttga actataagtt gccccactta tagagtctac atctgaactt tgcttcttta
     4261 ttatttcagg tatcaacttt ttagcttcat ctaaataact ttcatcatcc tctgaattta
     4321 gaacatctat ttttttaatc tttccatcct gtacttcaac tgataccttt gttttgccat
     4381 tttcaaatcc ttctccttcc ccctcaaaag taccgtcttt aaaggtagct gaattagcag
     4441 taatactaac cctttcactt ttattatctt ttatatccat acctttttct gaacacccag
     4501 aaagtgctac tcctaaggaa acaattccta tcgaaaaaat aactttttta ttcatctcaa
     4561 tactccttaa aaagttttag tatcttatat caaatttact actttttaat ataagattta
     4621 ttcttaatgt aaataaatta aaattaataa cgcttatact ttaaatatca ctaccatttt
     4681 gaaaagtttc aatatggtag tgatatttat tatttaagat aatagttatt tttattaata
     4741 tttaaatatg cattttctag ttatacaata ttgattctca ttatcaatat gttaattgat
     4801 tcatacattt aaatttatta aaaaccatct tatttaataa aataactagt ttttcttaaa
     4861 ctttttcata tttaacttta ggaaagctgc taaagaaatt aaaattccac ctatataagg
     4921 caagctacta gcttcccctg tttttggtac atctttaata tttttagttt cagctttgct
     4981 ttctctacta ctcataactt tactttgatc ttttatcaca tttttattgt tttctttttt
     5041 taccattgat atttcattcg gtttactttt atttaattca tctttattca attctttttc
     5101 agtattttta ttataatgtt ttgcgtctgg tttctcttca ttctgtttaa catctacttc
     5161 aggctttttc tcttcaagtt taacttgtgg ttttcctgta tccggattgc ttaaagtttc
     5221 agttttactt acatctgatt tcttttcttc cgctttttta ctttcatcat actttttgcg
     5281 ttcttctttt ttactttctg cttctggctt tttctcttca ggtttagatt gtggttttcc
     5341 tgtatccgga ttgctcggag tttcaggttt acttgcatct gttttctttt cttccgcttt
     5401 tttactttca tcatattttt tacgttcttc ttttaatatc ttatgtattt cccttaactc
     5461 attaatttta ggatttgttt tacaatactc ttttaacttc tcaggctcat acttaagctt
     5521 tttcatttca ttttcgtata catatatgta ctctacagga tctttaaatt tctttattat
     5581 atccttatta aagtctgctt tgaacttata tatatgataa atctgcttaa tttccttaaa
     5641 catatttaat gtatcttctg ctatatcttt actttctgct tctggctttt tctcttcagg
     5701 tttagcttgc gtttttcctg tatccggatt gctcggagtt ttaggtttac ttgcatctgt
     5761 tttcttttct tctgcttttt tactttcagt aatttttttg acttcctctt tttttgcttc
     5821 tctaaaaatt tcattgattt tttttattgc ctctgtgtaa taatttctta ttccagatgg
     5881 atcttcaatt ccattcattt tttcaagata ctcagatttt atttttaaaa ttttctcttt
     5941 atatacaggt gctttatctt tgttattttc actaaataat ttagttattt cttctatttt
     6001 attgtaagta ttgtcttcta agtttgttat cattgctcct tttgaaagac tttctgcatg
     6061 tacaactata ttaggtgtca caacacttat tgtagataat gctaatgagg ctgttacacc
     6121 tatcattgct gttttatttt ttaaattttt tatttttctt aatcttcttt ccattcttgt
     6181 cattaaaaat cctccttttt agcttgaagt ttcccttaga attttgctat gatttattta
     6241 attacaatat gctttcaaag ttttcagtta cccttatagt tccattctta tttataaata
     6301 gtccttccac cctatctata gaatttataa gttttatacc ttctttttca ccaagcgata
     6361 atgctattgt acttaacgca tcaccatcca tagaatcatc agatactata gtgacttgaa
     6421 ctaaatcgct ttcaaagggt cttccattct taggatttat tatatggtga tatatcttat
     6481 cttctttttc aaaaaatctt tcatatattc ccgaagaaac aattgattta tcacatactt
     6541 ctaaaattcc taatacctca cccctctctg aaaaaggcct ctgaattcca attttaaatt
     6601 tttctccgtt atctttttta cctatacata atacattccc ccctaggttt attattccgc
     6661 tacgtactcc ttctgaaatt aaatactctt ttattttatt tgctatatat cctttagcaa
     6721 ttccacctaa atctattgac ataccactta ttttaaatct gacattattt ttattaacat
     6781 ctatattttt ataatttact agctctaaat ttgtttcaat atctttctga ctaggaataa
     6841 tcttttcttc atttttaaaa ttccaaagcc ttaaaagtgg ctggatacta atatcaaagt
     6901 ttccattaga aatttcgctg taatataaac tcttttttat tatgttaagt gtttcatcat
     6961 gaacatcaac cccatctaca ccactgttat ttacattgta gatatcacta ccttttttag
     7021 ttttactaag aagttcctca taatatttgc ataaatcaaa agatttgttt aatatatcat
     7081 cacctttact ctgacaacaa catatgctaa tttccacaac tgtatcaaaa taaaaacctt
     7141 tcataaaaaa cttattttca ggtgagttat cccttacatt taatgtctct ttcatttaac
     7201 ttttccgatc ctcctaactc tatttttaaa cgaagttgat tattattatc atttaaaaaa
     7261 taaaaaattt aatgccatta tctaagatag tatcccaata aactctataa cttattaata
     7321 atactaccat aactcaattc attattttta tcattttttt cttaaatata taaaaaaaaa
     7381 gaaagatcct tacttcttta tatgttatat tctcattaaa ctaaattttc tttcataaga
     7441 ttacatagtc tgtccacact actttttagt ataataatta attgtatcat ttcagtagtt
     7501 tgcacagact atttataagt tatctagttc cacccttcat ttattttata ttttaaagac
     7561 taatataaat taccatcttt ataattcaaa aactatatct atgttttatt tggtaataat
     7621 actaatcaaa atatcaatgt gaagtatctt agtataaacc atataccata actatgaata
     7681 taaatactat aatggctata aatatatagg ccaaaggcat aaccatactc ctctcaattc
     7741 cataatctga atatagattt aaattcaggt taaagcttaa taagttctat ataagtaaaa
     7801 aattatctat tttcaaattc tgcatataat ttatcaattg ccgctatgaa tccatctact
     7861 ccatcttctc caacttctgc tccaacagaa tgtccaaagt agtactgtat atcatctatt
     7921 attgttttta tgttttcatc cgaatatcca gcgaagtttt tatcaacaat tccttttagt
     7981 acactaatcg gtccatgtat gtaatatctt ccataattag ctgatgcaat cttttctaac
     8041 tttggaaaca tattttttac tttttccttt agtgtagaat tcatttgatt ttgctcgtgc
     8101 tttgatttat tttcaaattt tgaatataat ttatcaattg ctgctatgaa tccatctacc
     8161 ccattttctc caacttctgc tccaacagaa tgtccaaagt agtactgtat atcgtctatt
     8221 attgttttta tagtttcatc tgaatatcca gcgaaatttt tatcaacaat tccttttaat
     8281 acactaatgg gtccctggat ataatatctt ccataattag ctgatgcaat cttttctaac
     8341 tttgggaaca tattttttac tttttccttt agtgtagaat tcatttggtt ttgttcatgt
     8401 tttggttttt cagtctcatt tggctttaat tcatttaata ttctctctaa ttcacctgct
     8461 gagtttagcg cttcatttaa tgttttatcc attgactcag tcgcagaatt taatgcttca
     8521 tttactttat tagtatcttg tgctttattt atttctatat tgcctacttc atagattgca
     8581 ttaatttcag caaatgattt tccaagtgct ttatcaccta atttttttaa ttctttattt
     8641 gcttcttcaa attttgcttt aagcttactt tttgcatttt ctctagcctc atttactttt
     8701 tcagtagatt tgttattatt aacttcatgg ttactttttt tactttctaa cctagattct
     8761 cttgccattg tactaatttc ctttgcctca acacgttgag gaacaataaa gttgctaact
     8821 ccgcaactta atgcagctga tgctattaat atctttactt ttttattcat tcttttcatc
     8881 ctttcttttc tttacatttc taaattaatt ataattttgt acttttaaaa tactataatt
     8941 taacatctat ttaacctcaa cattttctaa tttttaaaaa aaatattaac caagaaattt
     9001 tagaaaagtt gatatgataa gaaagaaaaa tttaactact tttttacttt taagtcctat
     9061 taacatccct ttttataaaa actaaaaagc acatttataa tttataaatg tgctttcata
     9121 gatttaataa tactaacctt tctttatctt tatgaaattt actggcttac aaattctgcg
     9181 cctctttgtg ttttaacatt taaagtttct aatcgtattc taacactatt aaatgttttg
     9241 tctttatatg cctctatttt accaattact tctatccaat catctggatt tagcatcgtc
     9301 attttatcat ttcctacaaa ctcaatacat actattccat catttccaca gcatcccggt
     9361 gaatgcctat aaacatagtt aagagtttta ccatcagcct caagagagtt atataacccc
     9421 tctattttta tagttctacc aatataatca tctgaattat taaaaatatc atcaagttga
     9481 gctataaaca ttttttcttt aatttctata ggagtcccat cattagaagc aatggaatct
     9541 tttttatttt ctattttatt agtatcgtta cctggatcaa ctatattttt atgattttct
     9601 actgttgctg gagtacttga gcacccagta agtaataata aaaataatac tattattaag
     9661 tttcttacta ctttcatatg taactcctat tattactttt tattttctct cctattatag
     9721 ataatataaa aataattata tttactacta caatacttgc gcctgtaggt gttgaaaatt
     9781 tatacgatat agtcatacca atacaaaatg ataatataga tactagcccg gaactaatta
     9841 ctacagactt aaaactttta aatattttca ttgatattat agatggaaaa ataattagac
     9901 ttgatataag catggctccc atcattctca ttccaagtac aatagttata gctgttaaaa
     9961 tagctatcat catattataa aaatttacat ttataccagt tgattttgta aagttttcat
    10021 caaaggtaat tgcaaatatc ttattataat aaatagtaaa taaaaatata actactattg
    10081 ataaaactac acttaaatat acatcatttt tactcatagc taaaatactt ccaaacatat
    10141 aattgcaaac atctgtattc attccagttg ttatagctat tgatattact ccaatagcta
    10201 gagaactgga tgatataagc gctatagcag aatcaccttt tattttacta ctctctttaa
    10261 ttctcaataa taaaaatgct actattataa caataggtat tgatagcttc aatggtgaaa
    10321 cacctagagc tacagctata gctatagctc caaatgaaac atgggatagt ccttccccaa
    10381 tcattgagta tttttttaaa actaaactta cacctaataa ggaactacat aatgatacta
    10441 aaattcccac tatcacagct cttacaataa atggataaga catcatttct gacactaaat
    10501 taatcattaa tctcaccacc taaaaacttt tttccacaat cactctgctt atagtctttt
    10561 acatctccat aaaacaacat tctatttttc atatgtacta ctttattagc ataatttaaa
    10621 gatgttttta tatcatgaga taccataata atagaaagtc cgtagttttt atttaaatta
    10681 tgtagcatat cataaagatt taatgtagtt ttttgatcta atccagttat aggttcatcc
    10741 attacaatca acttgtctgt ggcgcataaa gctcttgcaa taagaactct ctgttgttgt
    10801 cctcctgata aatcactata gcacttattt tttaaatcag atattttcat tttttccata
    10861 ttttcatgag ctagaacttt atctgatttt ctataaaatg ggaatacact atttcttttt
    10921 atacacccag ataatacaac ctcataaact gttgctggaa agtttctttg tatatcactt
    10981 tgttgtggta tatatccaat agtcgaatct tttaattctt cacttatgaa aatactccct
    11041 ttacatggct ttactaaacc caagattccc tttatcaatg tacttttccc tgagccattt
    11101 tcacctataa tgcataaata atcccctttt gaaacagtaa aactaatatc ctttgctaca
    11161 agtttatttt catatgaaaa actggtatta ttaaactcta ctaagttcat acttattgta
    11221 aaccttcctt cagattacct atattattgt tcattatatc tatgtatgta attcctttat
    11281 caaaatcttc ctttgtaaca ttgtgtgctg aatgtaataa taaaacttta gctcctgttt
    11341 cgtcactaat agttttagct atttttttat tactcatttc aatatgatat ataactggaa
    11401 tcttattttc tcttactgtt tttattaagt atgtaactgt atcaatactt ggttctgttt
    11461 gtgtactaca tccgttaaaa gcagccctat atgttaatcc ataatccttt gcaagatatc
    11521 taaatggaaa tttatctccc aaaacaatca tctttctttt agctttagct actgtatttg
    11581 taagtgattt atctagttca tcaagttttt tcgtataatt ttcatagttt gcattgtaat
    11641 atttctcatt tgtattatct actttaacta aagtattttt aatagcttct accatattct
    11701 ttgcattttt aggtgatgtc cagatatgtt catcatactc ttcgtcatga ttgtgattgt
    11761 cattctcatc atgtttatgt gatttttcct tatcgtgttc atgatcatgg tcatgttcaa
    11821 cttcaggttc cataccttct acttcctctt ccttaacagc ttttacataa tccatcatct
    11881 taatagcatt tacctttttt gtatctacag agtctattat tttttcaatc cattcctctg
    11941 cttctcctcc attataaata aatatatctg cattttgaat tttctttata tcttctggtg
    12001 ttggctcaaa agaatgacta tcttgccctg gagataagat catttttaca tctgccctat
    12061 ctccagctat ttgctttgca aagtcatatt gaggaaatag agtcgtaact atttttaatt
    12121 tatcttttct tgaactgata ttatttttgt cgttttcttt actttcagtt ctagtacatc
    12181 ctgttaatac cgacaatatt attaaaatta tagacattcc aataacagta acttttctca
    12241 ttttacacta cctttctaac ttatctctac agttttcaca tataccatag aaaacagtct
    12301 ttgtattatt aattttaaag ttatgatctt ttaatatatg attatttaaa tcttctagat
    12361 aagaacattt taagtgaatt agttctttac attctgtaca ttttaagtga aaatgttgat
    12421 ggcaatcctc tctttcacct atatattgat aacatgatcc tataccttct tcaatataat
    12481 attttcttaa aataccatca tcagctaatt tttccatatg tctatataca gttgttcgtc
    12541 ctactttatt accctgctca cttaaatagt taaatacttc gtctattgtt acatggctat
    12601 cttcatttga ttttaaataa tctaagatta tctctttttg ctttgtttta tatacagttt
    12661 tattattcat gaataatacc tcctgaaatt atattattaa ttattataat atctgtcaat
    12721 acgaaattga aattcaattt caaattttaa gtattatatt atacttattt acatataata
    12781 cttatattaa gtaaactatt tacaa
//
LOCUS       DAGSWI010000119        12669 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.119, whole genome shotgun sequence.
ACCESSION   DAGSWI010000119 DAGSWI010000000
VERSION     DAGSWI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12669
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.119"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..1525
                     /locus_tag="MJF80_002991"
     CDS             <1..1525
                     /locus_tag="MJF80_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBZ0281631.1"
                     /translation="IYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILLTLFSINVF
                     ISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCVMACFDLCR
                     LKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLENREQKIPYL
                     VNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMARLKSKGLKH
                     IEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIGSEALIRWL
                     HPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPVSINLSRVE
                     LYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSLGMNISLDD
                     FGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLNVTVICEGV
                     EDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDKCMTVEEE"
     gene            1528..1926
                     /locus_tag="MJF80_002992"
     CDS             1528..1926
                     /locus_tag="MJF80_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBZ0281632.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            1966..3699
                     /locus_tag="MJF80_002993"
     CDS             1966..3699
                     /locus_tag="MJF80_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBZ0281633.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            3931..5142
                     /locus_tag="MJF80_002994"
     CDS             3931..5142
                     /locus_tag="MJF80_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBZ0281634.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            5335..5595
                     /locus_tag="MJF80_002995"
     CDS             5335..5595
                     /locus_tag="MJF80_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBZ0281635.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            5949..7661
                     /gene="ptsP"
                     /locus_tag="MJF80_002996"
     CDS             5949..7661
                     /gene="ptsP"
                     /locus_tag="MJF80_002996"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBZ0281636.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            7984..9534
                     /locus_tag="MJF80_002997"
     CDS             7984..9534
                     /locus_tag="MJF80_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBZ0281637.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            9560..12262
                     /locus_tag="MJF80_002998"
     CDS             9560..12262
                     /locus_tag="MJF80_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBZ0281638.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIG
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVQVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSVQQIVHKQCANDILSCIENCGLDKKYICVEVTETVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQIAKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
BASE COUNT         5127 a         1121 c         2196 g         4225 t
ORIGIN      
        1 aatttacagt ttaaataaaa taatttttat tataattgga attttaggaa tagttttact
       61 cacaagacat attgaggtag atattaaagt tttaatttta attttattaa ccttgtttag
      121 tataaatgta tttatttcat atcttaaaat taagttgtat gaggataaaa ttaatttagg
      181 attgataaat aattcagata agtttataaa tattactaaa gatgagaact tcctaaaaca
      241 tgttcaaaat tatattatca gtaatgaaaa agaaaactgt gtaatggctt gttttgattt
      301 atgtagactc aaacttataa atgatatata tggttatgag ctaggggatg aagtgttaaa
      361 taatatttta agtaacttaa aaaattattt tggaaaagaa gctatttatg gaaagttaaa
      421 aagtgatgtt tttgtattaa taataaagct tgaaaatagg gaacagaaga ttccttattt
      481 agtaaatctt attaagaata aaattgtgat actaagtcag attgacagtt ttaaaatgga
      541 tataaatatt gaggcttcta ttggaattta taaatttaac aataatgaaa ttgatataaa
      601 aaaagctata gataatgctg atatggcaag gcttaaatca aaaggactaa agcacataga
      661 gtatgtagaa tttgataatg ctatggaaga agaaatacaa tcaattatga aaatagagag
      721 agatttattc ttggctatta aaaataaaga gtttgtaatt cactatcaac ctaaagttga
      781 ttcaagtaca ggtaacatta taggttcaga ggcattgata agatggttgc acccatcatt
      841 aggcatggtt ggaccaaata agtttatacc tatagctgaa aaaaatggtc tgataaacaa
      901 tattggtaga tggctaattc aagaagtttt tattaccatt aacaagtgga tttcagaagg
      961 tataaatgtc ttaccagtct caataaatct ttctagagta gaactatata aaaatgattt
     1021 agttgatttt tttaaactga tgtttaatac atataatatt ccaaaagaac ttatagaatt
     1081 agaaattaca gaaacgacag cactaagaga tgtaaaattt ataagtgaaa ggctttatga
     1141 gattaaatct ttaggtatga atatatcatt agatgatttt ggagtaggta attcaaattt
     1201 tatcaatctt aaggggatac cactagatat tataaaaatt gacaggtcac ttatattgga
     1261 tatagttaca aatacaaaaa caaagtttat ggtgaaagca attgttaacc tttcacatga
     1321 tttaaacgta actgttatat gtgaaggggt agaggatatg catcaggtca aagtcttaag
     1381 tgaactggga tgtaatttca tacaaggtta tgtatttttt aaacctttgg atgagatgaa
     1441 ttataaaaaa cttcttactg acggaagtta tataaatctg aaagatactc ttttagataa
     1501 atgcatgaca gttgaggagg agtaaggatg atttcagaag atgtagatta ttcaataaga
     1561 atcataagag ctatatttaa taatggagaa gataaaccaa tagatgctaa aatgatatta
     1621 aaaagtgaag atataccaat actaacacta tacagactgc ttagaaagct ttgtgcaaac
     1681 aatattctta cttttaacaa agaagatggc aagacataca tgttaaatgt gtcattggat
     1741 acaataagtc taagaactat tgtagaagta attgatggaa aaatatatat aaataaatgt
     1801 acgagatatg tatctaattg cagtaattgc ttaaaaggtt gtaaagtaaa tcgtgaattg
     1861 ctagaaatag aaaaagaaat ttataaactt ttatcgagaa aatctataag acaaatacta
     1921 gaataaaaaa ttttcagcta tgataataat tggaggtttt cggttatgga tgatatatct
     1981 caagataatt ttctactgtc taaagagtat gagaatagct tagatgtaga tactaaaaaa
     2041 gcaagtggaa tatattatac tcctaaaata attgtagatt atattgtaaa aaaaacttta
     2101 aaaaatcacg atataattaa aaatccatat ccacgaatac ttgatatttc ctgtgggtgt
     2161 ggaaattttc ttttagaagt atatgatatt ttatatgact tatttgagga aaatatatat
     2221 gaattgaaaa agaagtatga tgaaaattat tggactgttg ataacataca ccgtcatata
     2281 ttaaactatt gtatatatgg tgctgatatt gatgaaaaag ctataagtat attaaaagat
     2341 agcttaacaa ataaaaaggt agtaaatgat ttagatgaat ctgatataaa gattaatcta
     2401 ttttgttgtg atagtcttaa aaagaaatgg cgatataagt ttgattacat agtgggtaat
     2461 ccaccctata ttggtcataa aaaacttgaa aagaaatata aaaaatttct cctagaaaaa
     2521 tatagtgaag tttataagga taaagctgac ttatattttt gtttttataa aaagattata
     2581 gatattttaa agcaaggagg gattggcagt gttataactc caagatattt tttagaaagt
     2641 ctatcaggta aagatttaag agaatatatt aaatcaaatg tcaatgttca agaaatagtg
     2701 gattttttag gtgcaaatat atttaaaaat ataggggtat ctagttgtat tctaactttt
     2761 gataagaaaa aaacaaaaga gacatatata gatgtattta aaatcaaaaa tgaggatata
     2821 tgtataaata aatttgaaac attggaagaa cttctaaaga gtagcaaatt tgaacatttt
     2881 aatataaatc agagattgct atctgacgaa tggatacttg taaataaaga tgatgaaact
     2941 ttttataata agatacaaga aaaatgtaaa tatagcttgg aagatatagc aataagtttt
     3001 caaggaataa ttacagggtg tgataaagcg tttattttaa gtaaagatga tgtaaagttg
     3061 aaccttgtag atgataaatt tttaaaatgt tggataaaaa gtaagaatat aaataaatat
     3121 atagtagata aaagtgaata taggctaata tactcaaatg atatagataa tgagaataca
     3181 aataaaagga tactagatga aattataggg ctatataaaa caaaattaga aaacagaaga
     3241 gaatgtaaaa gtggaataag gaaatggtat gaacttcaat ggggtagaga aaagcttttc
     3301 tttgaaagaa aaaagataat gtatccttat aaatcaaatg aaaatagatt tgctatagat
     3361 tatgataaca atttttcaag tgcagatgta tattcttttt ttataaaaga agagtattta
     3421 gataaatttt catatgaata tctagttgga attttaaatt ccagtgttta tgataaatac
     3481 tttaaaataa ctgctaaaaa aatgagtaaa aatatatatg attactatcc taataaggtt
     3541 atgaagataa ggatttttag ggataataat tatgaagaga tagagaattt atcaaagcaa
     3601 ataatatcaa ttttattaaa taagtctata gataaaggaa aagtagaaaa attgcagata
     3661 aaaatggata acttgataat ggattcttta ggtatataag cagttgtaag tattaaaaaa
     3721 ttataatctt ttaattgttt atttaaattt agaaataaaa agttagccat ataaaaaata
     3781 ttaaaaatat atacttttca atattttttt gataaaataa gaatgaattt gtgcaatttt
     3841 atttttctcc aataactaaa aatagttttt tgaattaata tttgcttaag aataaaaatc
     3901 agaaacgttt ttgatatagt aggtgataat atgaaaggat tagttttaga aggtggagga
     3961 acaaaaggag cctaccaaat aggtgcatat aaagctttaa gagagcttgg tatagagttt
     4021 caaggagtaa ctggcacatc aattggtgct ttaaatggag catatataat tcaaaatgat
     4081 attgaagtaa tggaagatat ttggttaaat tatgactata ctcattttat gaatattgat
     4141 agagaaacct atgagaggtt taaaaatgta gatttcacta ctaaaaatct caatgcagtg
     4201 tttgagctta taaataaagc tagaaaaaat gaaggtatag atataagccc attaagacaa
     4261 ttaatggaga caacattaaa tgaagacata ataaggaaat caaaaaaaga ctttggactt
     4321 gttacggtct ggtgggataa aaaaattaat ccatatccat tgtatatcga agatatacct
     4381 aaaggaagat tgattgatta tttaattgct agttccagtt tgcctatttt tcaaacgaac
     4441 atgatggatg ataagttgta tcttgatgga atgttttgtg ataacatgcc aataggactc
     4501 ttggaagaca aaggttatga agatgttgtt gtaattaggt tgattgatga ttttattggg
     4561 aaaatgaatc taaatcgaca tcaaaatata aatataaaaa ctataatacc atcagaatat
     4621 ctaggaggtt ctcttaataa agatagagat aatgtagaaa aaaacatccg actaggttac
     4681 ttagatacta tgaaagcata tgaaagatgc actggaatta agtattattt tgatgtagag
     4741 tataaatata atgaagaata ttgttttgat aaatttagaa gtatgagcaa tgaaacaata
     4801 caatatatct gtaatttatt gaatataaaa agagatgctt cacttagaac tgttatggaa
     4861 agtgtaatac caaagcttgg tgaggtgctt ggattgccaa aggatttttc atataaagat
     4921 ttgttttact gtatatatga aaagaaactt gaagaaaata atataaacag gataaaatta
     4981 tatgatttta acaaggttat agatgttgta aatacaaata ttgattctaa tagaaattta
     5041 gcttctaatt ttgaagttag acctaaaatt agtattaaaa agaacaaaaa cacaaaatta
     5101 ttgacaaatt gtattataaa agacataaaa aatcaaagtt aaataaaaaa caaaaaaaaa
     5161 tttgtaatta tactcgaaaa aagtgtatat tagtagtata atatttttgt agcactagtg
     5221 ggaacaaaaa atgaccatag gacaaaaaag taccacttag taccattaaa gtaaaaaaca
     5281 ggaaaacaat tttaaaaaat ataaaatgta aagtttatag gaggatttaa aaaaatggaa
     5341 aaagtagtta gtatcaaaaa tgcaagtgga ttacatgcta gaccagcagg aatgtttgtt
     5401 aagaaggcag ctgaatttaa atcaacagta gaggttgtag caaaagacaa aactgtaaat
     5461 gcaaaatcaa taatgggaat aatgagttta ggtttagctc aaggagaaga attaaagtta
     5521 gttgtaaatg gagaagatga agaagtagct ttagctgcaa tggttgaatt agttgaaagt
     5581 ggatttggag aatagatata ataatttttt aaaaatttga tatttagaat ctaatattac
     5641 ttaaagtaaa gtaacagaga agtattaaac atatacagac caacatttgt agatatttaa
     5701 tttctttgtt actttcatgt ttatttaatc agaagattaa gactattgtt aattgggtat
     5761 aaaaatataa atattttaga aaatgaaata agatattttt aatcataaat tcagagtaaa
     5821 aatacagtaa caaatagttt ataaaatttt ataaaaaatt ataagtaaat aaaactataa
     5881 aaatgtaaat tatttatata aatttataaa gtttatgtgc tagatgatat tttttaggag
     5941 gtagggttat ggcttacaaa ggaataggtg cttctcctgg tgttgcttta gggaaagcgt
     6001 tagtagttga acatagtgaa ttagtaattg aaaaaaaatc tatagataat gttgaagctg
     6061 aaattgctaa attagaagat gcagttgcag tttctaaaga agaattagta aaagttaagg
     6121 aaaaggcttc tgaagaatta ggagcagagg aagcagaaat atttgaagca catttattag
     6181 ttttagaaga cccagagtta attggttctg caatagataa aattaaaact gaaagtgtaa
     6241 atgcagagta tgctttaaat gaaataaaag agatgtttgt tagcatgttt gagtctatgg
     6301 acaatgagta catgaaagaa agagcggcag atataaaaga tgttacaaat agaatactta
     6361 gacatatatt aggaattaaa gttgttgatt tatctgcttt aagcgaagaa gttgtattaa
     6421 tagcacatga tttaactcca tcagacacag caactatgaa taaaaaaatg gttttaggat
     6481 ttttaactga tataggtgga agaacttctc atactgctat catgtctaga actttagaaa
     6541 tagctgcaat cgttggatta aatgatataa cttcaaaagt taaggatgga gattttgtgg
     6601 ttttcaatgg agacacaggt gaagtaatcg tgaatcctga tgaagagact ataaataaat
     6661 atactgaatt aaaagctaag tatgaagatg agagaaaagc attacagcta ttaaaaggaa
     6721 aaccatctgt aactttagac ggaaaacatg tggaattagc tggcaacata ggaacaccta
     6781 acgatataga aggtctaata aagaatgatg ctgaaggtgt tggtctttat agaacagaat
     6841 ttttatacat ggatagagat tctttcccaa cagaagaaat acaatatgaa gcatacaaag
     6901 ctgtattaga aggaatggat ggaaagccta tagttataag aacattggat ataggtggag
     6961 ataaagaatt aagttattta agtatggaac ctgaaatgaa tccattctta ggttacagag
     7021 caataagact ttgcctagat agaaaagata tatttaagac tcaattaaga gctttatata
     7081 gagctagtgt tcatggaaga ttaagaatca tgttcccaat gatttcatct ttagaagagt
     7141 tgttacaagc taaagaagtt gtaaaagaag ttttagcaga acttgattct gaaggtgtgg
     7201 cttatgctaa agatgtagaa attggtatga tgatagaagt tccatctgct gctgtaatat
     7261 ctgatgtatt ggctaaacac gtagatttct tctctatagg aactaatgat ttaattcaat
     7321 acacttgtgc agttgataga atgaatcaaa agataagtta tctttacaat caatttaatc
     7381 cagctgtttt aagacttata aagactgtta tagacaatgc acataaagaa ggaaaatggg
     7441 caggtatgtg tggagaatct gctggagacc aaaaaatgat accaatactt ttaggtatgg
     7501 gattagacga gttctctatg tctccaatat caatacttcc tgctagaaag ttaataactt
     7561 cagttaaaga gtcagatatg aaaaaattag ctgatgatgt acttaatatg ggtactgctg
     7621 aagaaataaa aagctatata gaaaagactt ttaatatata attaatgtta tatagttact
     7681 aaaattcatt tttctaaata acaaaattat attttacgaa taaaaaaact atagtatatc
     7741 atatatacta tagttttttt gttatataaa ttacgtatca aaatttgtaa aatgaataga
     7801 tataattact aaatatgtga tagtattggc tataatagga tataaaaaat aatattgtaa
     7861 taaaaagttt atatttagta atgaaaattt aacgttttac ttgaaattat gtgttttgta
     7921 atgtattatt aaggttctaa taggaacatt ttcatcaatt tttctatagt gggggtagga
     7981 attatgaggt acaaaaaaag aaatatagat aagtcaagtg aactttcatt tcaatctcta
     8041 gcaaaatatt ttataacttt ctatatagta attatattaa ttattctttc tatgttttgt
     8101 ataaatacaa tatgggttat gaaaaaagat aatcaagatt tattatctac aacatcaaat
     8161 cgtattgaag atagggtagg agaaacaatt aagctattga actctttgag taaagataat
     8221 tacataatag ataatagtgt gaatggcata gataaaagta aagaactttc aatgtatagt
     8281 gatgaatttg ggtatatgat gatatgtttt gttgataaga atataattgt gtgggatgaa
     8341 gaaggtggta atattagtct agctggtcgt gattatatgc agaaactttt tgcaacaaaa
     8401 aaatatcaag ttacagatag ttttgcagca ggggctgatg gaaaaacttt aaactacact
     8461 attgcatcac ctataataaa gaatggaaat gtagatggtt gtgtatttgc agcaatttac
     8521 tttgatgaga taaaggatat actcaaacaa ggaaaaggtc aacagcaaca gaatttcata
     8581 ttatttggaa gtgaaaatca aataatgaat ttaacaagtg atgaaactgc atatggagag
     8641 aagtatatga agttatcgca gaaaacaaaa tatcttggaa ccaatgcaaa aaaagtagaa
     8701 aaggatatgt ttaataataa atcaggtgat tattgggctt ttgaaaatgg aaaattatac
     8761 tttatgtcct ataaaccaat taagaacaca aattggagtt tattttgtaa agttaatttc
     8821 ttagatattt ttgcattaaa tttaattcct atgggagcta ttctatctgt tataacttta
     8881 gtatttatta ttttgatttg tatggagaaa agcttcatga aaaaacgaat ggaagttatt
     8941 gatatgcttt taggctctgt tcaagaatta gaaaaacgta tatatcaaac agaaagacct
     9001 agtgaagtag actttaaaga aattattcag ctcacaagta aaggtctgac tgatggatta
     9061 actggtgttg taacacgtac tgtatttttt gaccaaattt ataatcgtgt tgaaatggag
     9121 gaaaatgata aattatctgt aatttcattt gttgatttag atgatttgaa atgtattaac
     9181 gacttatatg gacatgaaat tggggatatg gttttgaaaa atgttggata tgtacttcgt
     9241 gaatatgaga taaaatataa tggtttaatt gggcgctatg gtggagatga atttgttatg
     9301 ttgttatctg gttttacatc gagagaagag gtggaggatt tattggatga gattgctatt
     9361 cgcttacata ctgaagtact aacagatgat ggtatcattc aaactcattg tagtgttgga
     9421 gctgtagtat gtagtgtaaa agacaataaa gatatagata atttattaaa acaggctgat
     9481 gaagctttat actatgttaa acgtaatgga aaaggtaatt atcatttagt ttaatgtatg
     9541 tggaaggagt ttatatcata tgaaaaatgt atctaaaaaa ttttttgtat gtactattat
     9601 tgtattaata acatttttat gttttagtaa atttgctatt tatgtttttg acaaacaaga
     9661 aaagaaagaa acaaaagtag tagaaaacat gttaaatgtc tacaagaata acattatttt
     9721 aaatctgact gaaaaattta attatagcaa agacttgaca tcagttttag tttcaaacaa
     9781 ttattctata gataaattaa aacctattgc agatgaaatt cttaaaaaca attcttgcat
     9841 atatatttca gttgtagaaa aatataatgt agctgcattt ttttctaatt ctgtcaaaaa
     9901 agatattatc aatactccaa tacaggaact gaattattct tattcattag caaattctat
     9961 caaaggatta gtcgtagaag gtccttctaa agaccagttt tcaaatggag atgtgttttt
    10021 aattactaat cctatttata aaaatggaga gtactgggga gaaattacag tagctttaga
    10081 tgccaaaaaa gttgtagatt cttttaactt aaataaatta aaagaatctg gatttgagta
    10141 tgaactttgg cgtgtacacc tagataatgg ttcaaaatct acaatagaaa tttctactaa
    10201 agattttaat ttttccaaat cacatgaaat taagtttaag cttccgactg tttggactct
    10261 tagcattgtg tacaaaaatg gatggaataa taagaatata gattttattg tatttgttat
    10321 attgttaatt atttccatat taataggtgt gatgacattt atttacattg aaaagaagtc
    10381 gattggtaag gaattaaatc aacagagtct taaaaattta gaaactggta ttttgaatag
    10441 agaaggtttt ttaattaagt tacaagagca tctgaataga aaaaataatg aaatagtatt
    10501 gctgtatata tgtattacta attatagtaa atttgctcca gttatgagct ttgaaaaaag
    10561 acaagattat cttacacata tagtaaaaaa tctagataat tacataaaaa gttctcattt
    10621 tattgctcgt ataagtgagg aaggttttgt tctagcttta gaagaaaatt gtgataaaaa
    10681 tgaattgctt aacattgaaa aagggatttc cttagagctt ttacgaaaaa ttaaagtaga
    10741 aaataaacaa gtatttttaa cgattggtta tggctcatct aggtgtgttg atgatagaaa
    10801 agatgcagaa tctctgttac tttcagctat tgaagattat gagagaagat ttccagcaat
    10861 gtctcctatt catgatttta ctaaaaagtg tgaagatatg cttgatgaaa agaaacagat
    10921 tgtatttggt gaatatccag atttagaaat aaacaagttg tctaaagtaa tatacaaata
    10981 ttataaaaaa gtacagcgta ttatgtataa agattataat attgatattg gaaatagacg
    11041 agggtatatg cgtgatgttc aagtattaat tgattataat tctgagagaa tgttatactt
    11101 atttttaata gacatatgca agtttggaaa atttaatgaa ctgttttcta ttgaaatagg
    11161 agatgttatt ttaaaagaag ttagtaacaa attaaaaaca attttttcta attatctata
    11221 tcgtattaat ggagatgtat ttttaggcat ttcaatagat aaagaatctt acttagatac
    11281 tttacataag gttaatgaag tatttaatag ttctttaagt gtagaagatg tactttttga
    11341 tgtaaaatgt aatattggta tttgtcaata tccatatcat ggtgaaaatc catatgacct
    11401 tctggaaaag gtacaaattg cattggaata tgcaaaaaag gatgtcaaaa gtaatgttaa
    11461 agtttatgat aaggaattga cagaagagat ttaccatgaa aataatatta ttaaggattt
    11521 aaaaaaaggt ctggaagagc aaacactaga agtttggtat cagccaattt ttaatgttag
    11581 taaaaactgt tatataaaag ctgaggcact aattagattg agaaataaag agggcggtta
    11641 ttattctgct tatgaagtta ttacttatgc agaaaagcat ggacttatta aggatgtagg
    11701 tctatatgta ctaaaaaagg cttgtgatgt aactacaaaa ataaaagata aaaatataga
    11761 ttttgaacaa atgcaattaa atttatctgt tcagcagatt gtacataagc agtgtgcaaa
    11821 tgacatatta tcatgtattg agaattgtgg tttagataaa aagtatattt gtgttgaagt
    11881 tacagaaaca gtacttatgg aaacttttga aattgtaaat gttactcttg ataaactaaa
    11941 aaaagaaggc attagcatag ctttagatga gtttggttct ggatttagta gtataaatta
    12001 tctggcaaat ttacctattg aaaatttaaa aatagataag gaattagtag aacaaattac
    12061 ctattctaaa aaacaatttg aattattaaa aactatagtt caaattgcta agattaatgg
    12121 catggatatt gtagcagaag gtgttgaaac aaaagaaact ctggatttag ttgtaacttc
    12181 aggtgtgaat ttgattcaag gtcattattt ttcagaacct ttaagtgaag ataaatttat
    12241 tgaacttatg attgaagaat aaaggttaat aaaataaaga tttaaggtat tgaaaaataa
    12301 ttaaaagtat ttaataatag aattagggaa gaataagttt ataattaaaa ataattgatt
    12361 agtagttaat aaaataaatg tgatatccaa ataaatccta aatctaattt gagttttaat
    12421 atagatagtc taaagtttga ttattaaaga tttaattgta agttgttaca gttgatgata
    12481 ttgattagag attaaagttt agagtagaaa gttaccttaa atttaaaaat gcaagataac
    12541 ccaatctaaa aattataatc actcgatagg tttaaggatt tatttgttta ttataaatag
    12601 gagggtgtct caaaatgaac tttttagttt atgaggcact ctcttttata tagaattaaa
    12661 tatatttta
//
LOCUS       DAGSWI010000120        12660 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.120, whole genome shotgun sequence.
ACCESSION   DAGSWI010000120 DAGSWI010000000
VERSION     DAGSWI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.120"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            43..780
                     /gene="cas6"
                     /locus_tag="MJF80_002999"
     CDS             43..780
                     /gene="cas6"
                     /locus_tag="MJF80_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBZ0281639.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKEREFYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            785..2260
                     /locus_tag="MJF80_003000"
     CDS             785..2260
                     /locus_tag="MJF80_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281640.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFCELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDIVIKSN
                     IEIDEIDKLDLKQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRIDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     MNTRNLGKKMINNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGREEYKMKNYIESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2280..3296
                     /gene="cas7i"
                     /locus_tag="MJF80_003001"
     CDS             2280..3296
                     /gene="cas7i"
                     /locus_tag="MJF80_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBZ0281641.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYRDKVHA
                     KTSIATITYDLRRILHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKEKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEREENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEEDGKLLLPEERKFMSKELREK
                     AVKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGV
                     IETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMW
                     YLKE"
     gene            3312..4109
                     /gene="cas5"
                     /locus_tag="MJF80_003002"
     CDS             3312..4109
                     /gene="cas5"
                     /locus_tag="MJF80_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBZ0281642.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4136..6544
                     /locus_tag="MJF80_003003"
     CDS             4136..6544
                     /locus_tag="MJF80_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBZ0281643.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            6985..7932
                     /gene="prmA"
                     /locus_tag="MJF80_003004"
     CDS             6985..7932
                     /gene="prmA"
                     /locus_tag="MJF80_003004"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBZ0281644.1"
                     /translation="MNNWIEVTIKTTTESVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKGFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            7951..8709
                     /locus_tag="MJF80_003005"
     CDS             7951..8709
                     /locus_tag="MJF80_003005"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBZ0281645.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8711..10009
                     /gene="mtaB"
                     /locus_tag="MJF80_003006"
     CDS             8711..10009
                     /gene="mtaB"
                     /locus_tag="MJF80_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBZ0281646.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10052..10402
                     /locus_tag="MJF80_003007"
     CDS             10052..10402
                     /locus_tag="MJF80_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBZ0281647.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10565..10744
                     /locus_tag="MJF80_003008"
     CDS             10565..10744
                     /locus_tag="MJF80_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBZ0281648.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10774..11217
                     /locus_tag="MJF80_003009"
     CDS             10774..11217
                     /locus_tag="MJF80_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBZ0281649.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11328..11825
                     /locus_tag="MJF80_003010"
     CDS             11328..11825
                     /locus_tag="MJF80_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBZ0281650.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12018..12581
                     /gene="raiA"
                     /locus_tag="MJF80_003011"
     CDS             12018..12581
                     /gene="raiA"
                     /locus_tag="MJF80_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBZ0281651.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
BASE COUNT         5364 a         1113 c         2323 g         3860 t
ORIGIN      
        1 atagagaata atgaattata ctagaaagta ggaggtgaaa atatgagcag aatttcaatt
       61 tttatggaat catgtaatgg caaatttcca aatgaaaatt caatgttgtc agtttccttt
      121 ataaaaaata ttttaaatgc agaaaacaaa gattttgcta aaaacatatt taactatggg
      181 gaagaaagaa aaaacaataa gcaaataaag gatataaata cagccattta cataccaaat
      241 cttataagaa gcaaaaatga gttacttgtt gatggagata taatattcaa tataagtttt
      301 tataattatt ctatgttcag taaattatac aatggaattt taaaagtaaa ggagctagaa
      361 tataaaggtt atagatttaa aataaaaaat ataaaaatac ataaagaaca cgagataaaa
      421 gaaaatggtg ttatatttaa aactatgtct cctataatag taaagaataa agaaggtaaa
      481 tatttagatg tagaagattc aaattatata gaatgtttaa actatatagc taatctaact
      541 ctggagagta taagaggtag tgggcttaga aagccattgg aatttatacc tcttaacttc
      601 aaaaaaagag tgttaaaaga aaagattcgt ggatttaaag agagagaatt ttactatata
      661 aatgcttatg caggaacatt ttttttaaaa ggaaatatgg aagatttaaa tgcactgtat
      721 aaaatgggaa ttggatatag aaggacggaa aatgcaggaa tggttgatat attaaagtag
      781 cgaggtgaga gaaataaaag ctaaatttta tagatcaggt gatattgtaa gagatattgg
      841 cattgtttac ttttgtgaat tactaatgga tttaaaaaac gaactagaaa aaaatgatta
      901 taagctaaaa tttacttgtg agttgaatag aaattattta tctttagaat ctgcagaata
      961 tatagaccct aagtatatat ctgattatat attagaaaat caacttttta aggtatttaa
     1021 aaatgggaaa aaggaaacct tagaaaaaat atttagtaat atagataatt taacatatgt
     1081 aaattttatt gaagaattgg ataaatcttc tgcaactgag aaacaaaagg aagggattaa
     1141 aaaagatttt aaaaacagaa gatttccata tgttagaaat agtggtaagt atggattaaa
     1201 cacgagtcta gaaaatatgg aaacaaatgt aaaaagaata gtcgatattg tgattaaatc
     1261 aaatatagag atagatgaaa tagataaact tgatttgaaa caatatgaag aaaaagatag
     1321 tgtatgtaat gtttgcaata taaataaaac aacaaaacta gatattgatt taagagaacg
     1381 aaaggactct aagtataact ttttatttag aggtgcagaa aaatcaggtt ttaaaagaag
     1441 tggacaagtt gaaagtaata tttgttttga gtgtgaattt ttcaacttaa tgtgtctttt
     1501 atatataaat ttaaaaagac caatggtttt tgcatatacg aatgatttaa gagaattagc
     1561 atttttaaat cataagataa tgcttaaaag aaaaatgtat tcagataaat cattttataa
     1621 aaaacttttg catgaaaaaa taagttcaat tagactatat aggattgata ttgatacgaa
     1681 taaaggtata attctaaaat ttgactctat tatagagtat aaagaattat taaaaaaaat
     1741 tgaacttata gatattatag ataattataa tttttcaaga gagccaatga atactagaaa
     1801 tttgggtaaa aaaatgatta ataatgggaa tttatcaaat ttaaaagaat tattacttga
     1861 taatataagt gttttaaggc aactaactgg aacttctcga gaaatggatt ttgtatctag
     1921 ttcatataat atcggtttat atataaaatt gtgttggatt gtagatggaa gggaggagta
     1981 taaaatgaaa aactatatag aaagtaattt aatatattca agagtaggta aagacttatt
     2041 taataaaatg acagatgaat ccagaagaaa aaatttttct atgaaagtga ttcaactact
     2101 taagtctaat gatagaactc agctatttca aactataatg catgtgttag tttctaatgg
     2161 aatcttaata ggagaagggt ttgtagaagg tctaatgcag agttctgaat tagaattaaa
     2221 tacgaatgta ggtctattta ttcaagaaat catgaaataa aaaaggggga aaatttaaaa
     2281 tgaatgaaaa aagagtaaaa ggagtaacat taactatact gacagaaagc ccagttccac
     2341 tttcgtatga ccaagggtat ggaaattata caccaataaa aaaagaacaa tatagagata
     2401 aagtacatgc aaaaactagt atagctacaa taacatatga tttaagaaga atactccatc
     2461 aagaatatgg ttggaattta agtaatattg tttttggaaa aaaaggaaat atatatccat
     2521 ctattaagaa aaatgtagga tgtgtagatg aaaatggatt agagactgat gtatttggtt
     2581 atttaatacc acttaaagag aaaggttcta taagtaaggc ttctccgctt agaataatac
     2641 cttttaggtc attaaattca tatagaggtt caacacagtt gataacaaat agaggttttt
     2701 taagtagcga atttggtaga aaatattatg atgagaggga agaaaatgaa gtacttagag
     2761 atgaaaactt tccaacaaca caagcattag caatagaaga gactttatca gattactacg
     2821 tttatacaat aacattggag cttgatagaa taggagtagt agaagtagaa gaagatggaa
     2881 agttactttt accagaagaa agaaaattta tgagtaagga gttaagagaa aaagcagtaa
     2941 aagatatatt agatgcaatt actgtgttta caagaaacat aaaacattca tcagtactat
     3001 taaaaccact tgcagtaatg ggtggagctt ttgataaagt tgtaccattt ttttgggatg
     3061 atgtagatta taatgctgat agtggtgaga taaatttaga gggagtcata gaaactatag
     3121 aaagttatag ccttaaagaa agtaacacaa tacttgctat caatgacaga ttaaaaattt
     3181 ctaataaaaa ggaattaaat aagtttaatt taagtaaata ccctgtaaaa gagattaaaa
     3241 atctagcaga tagattggaa attggagaag ataatatgtg gtatttaaaa gagtaggagg
     3301 ttttttgaat tatgaaagta ttacaatgta aacttaagca accaactgct cactatagag
     3361 acccaaaagt atttcaaaat gaatatataa gtactttaaa tttaccatct aagaccacaa
     3421 taatgggaat gataacgtat ttatgtgata gaagattaaa ctctgatata gatataggaa
     3481 taattggaac acatcatcat agagaactag aattttcaag aggagagaac attgattttt
     3541 ggaatgaata tacaaatatg agaaaaggca aagataaaga aaagtttttg cttcaaggaa
     3601 actactatga ttactataaa gaacataaag ctcaaaatag catattaaac tatgaagttc
     3661 ttaaagaagt agaactgact atatttattt cttgcaagga tgatgaagaa cttgaattta
     3721 ttagaaaaaa gcttgaaagt ccatgtaaat atgcaaattt aggaagaaaa gaagattttg
     3781 taataccaag tgaaaaaggg tgttttgtga aagaagttga gttaaaagag gttataccta
     3841 tgaatactcg tgatgctata aaagaaaata taaaattaaa gaatacatat gtaagagttg
     3901 acttaagaga taaagagaat gtcgagacta taataaatca aggtgtattg attgcattac
     3961 cttataaata taaagattta gaagcaaata gagatgatag agtttatgag ttttgccatt
     4021 atatatatgt tgataatgat ggtatatatc ctaaaaatat taaggtaaat gtatgtattg
     4081 atacaaaaga agtttttaca tggctttaaa ttattttgaa agataggtgt ttatcatgaa
     4141 aaatatcatg caaaaagaaa tattagctaa gtcatataat ttaagaacta atcaacaaga
     4201 gcaaacatta gttgaacata ttaaggattt atttgaagta ttagaatcta tattagaact
     4261 aaatctatat tcagataaag atgtagagat actaaaaata tgttgtgctt tgcacgattt
     4321 aggaaaaatt aattcaatta tgcaacaaaa aatggagatt aataataaaa tatcgtatag
     4381 ttgctcagaa gaagagagga aaaagttaga gtctgacaaa aaatctttaa aaaagatagc
     4441 aagacataat atattttctg gagcattttt aaaagatata ttagaaaaaa tgaacttgtc
     4501 agaagaagat aagttatata tatataaaag tataatgtta catcatggaa actatgaaga
     4561 ttatatgagg ttaagtatta gtaaagttca agaagaaatt tatgagtata ttgaaaaagg
     4621 cattttagaa aaagaagagt ttaatctgaa agatatagaa agttatataa atagcatttt
     4681 aaatgtagac tttaaatttg gtgaagatgt attggattat gattacatag ataaattaag
     4741 tgaaagtatg tttatagaca gtgattataa caatggtcaa atagatgatt cagttttaaa
     4801 ttatagaaag tttaagtata ttttatataa agggatgctg aacttaatag accatagtgc
     4861 atcaagtaga cagaaaggta taaaatttta taacgatttt actgatgaag aaattgataa
     4921 tatgatttta aatgagatat ataagagtca gggtaatttg gaaaaaaata atatagagtt
     4981 caatactata caaaaaaggt tgagagaact ttctggaaga aatgttctga ctgttgcatt
     5041 tacaggttca ggaaaaacag ctgcagatta tagaaagact tttaaaagaa aattcttttt
     5101 agtgccaaat aaaatatcag cagaaagttt ttatagaaaa aatatttttc aaaataaaaa
     5161 tgatttagat acaagaagta gtaatgatta cattggatta cttcatggag atataaactt
     5221 atattcagag aatgaagata atggagaaca tgattttgta ttgacattaa gggatataga
     5281 tttgagtata aacttttgta aaccttgtgt aattgcaaca gttgaccagt tattacttag
     5341 tatgtttaaa tttccgggat atgaaaaaat attcgcagcc gtaaaaaatg catctataac
     5401 tgttgatgaa gtacatttat tggaaccaaa gatgtttttg attttgatat attttatgca
     5461 gtttgcatgc aagtacttag atgttgattt tcacttaatg acagctacac ttccaaaatc
     5521 atataaagag caaatgataa acaagggaat tatttttcaa gaagaaagta atgaaaatgt
     5581 tactgataaa ggagaaatag tttttattga gagcaacaag gaagaagaga tttgtgaagg
     5641 taaagatgta aaggtttctt ttataaagga aaaagaaatc aagtcaatag ttgaagatgc
     5701 tttagaaaat aaacaaaaaa ttcttataat aaagaatact attgattcag tcaatagaac
     5761 atatgagttt ttaaaagaaa atttatctga taagtatggt ggtgtagata tagatgtttt
     5821 acatagtaga tttaagttca aagataaaaa agagaaatat agtaaaatat taaatggaga
     5881 gggtgatatt tggatatcaa ctcaatcagt agaaatatcc ttggatttgg atttcaatat
     5941 aataataagt gatttagcaa ctatggatag cttgatacaa agaatgggta gatgtaatag
     6001 aaataataaa tatgaatatg gaaactttta tattctacct agtgaagata aaatttatga
     6061 taacaaactt aagaacacaa ctaaaaacat cttaaaggac atattaaaaa ctaactctat
     6121 ttttacaatg gggattagaa aaactatttt ggatgattac tatgataata gtgtagttaa
     6181 aaaatatttt gaagagaatt ttataagttg tgataaagaa attaagaata tttatggtat
     6241 aaataaagaa atttttgatg gattggattt aatttttaat tttgaacctt acaaaaatat
     6301 tgttgatagt aagaaggaag cagctaaaat ttttagagat gttgatgtta gttacaaaat
     6361 tattctggaa gaagattttt ataaggaaga tagggagtta caacaagatt cgattcaagt
     6421 atcagggttt atatttaata gattatatta ttatggatta ataagcaaag tagaaggata
     6481 catggtacta aaatctagta gtaagtttga gtataatagt actgtaggct taattttgaa
     6541 gtgagcatat gtttaaatat aatatacctt tagacattga aaatacaaaa agtactactg
     6601 taagtctact gtaaaaacta ttttttaaaa ggaaaaataa gttgtgagat agagtgttta
     6661 caagtgtata tagataataa atttgaggtt taagattaac taagtgggtt tcaggtacat
     6721 cgcgaaaata attataagtt ggagattttg tatgtttaag attaactaag tggatttcaa
     6781 atattgatat agtagatata atagaagttt tattattagg tttaaaatta actaatcgga
     6841 tttttaaata ttctgactat tatataagga tattattaga ttgaatttaa aaccaataat
     6901 ataaaacaac aaaatattat atttatgata taataaaagt tatggaaaaa caggaatatt
     6961 actatagaga agagaggtaa acttatgaat aattggatag aagtaactat aaaaacaaca
     7021 acagaatcag ttgaacctat aacgaatata ctatatgaac aaggagctgg aggagctgta
     7081 atagaagacc caaaagactt tttattccaa aagaaaaatg aactagactg ggattatgtt
     7141 gaagaagaag tatttaaaaa gaatgaagaa gatgatgttc ttataaaaac ttatgtttct
     7201 gaagaaaaaa atgtgatgga atttgtcgaa ataataaagc aaaaagtttt aggattaaaa
     7261 ggttttggta tagatatagg tgaaggaagt gtatcattgg accaagtaaa tgaagctgat
     7321 tgggcaaatg catggaaagc atactacaaa ccaacaaaag tgggacaaag ggtagtagta
     7381 aaacctacat gggaagatta tgctatgcaa gagggggatt taatcataga gcttgaccct
     7441 ggaatggcat ttggtacagg gacacatgag actactagta tgtgcattag agaattagaa
     7501 aagtatgtga ataaagattc taaagtattt gatattggat gtggtagtgg aatccttgca
     7561 atagcagcag ccaaattagg agcaaaagaa gttgtagctg tagatttaga tgaagtggct
     7621 gtaaaagttg caaaagaaaa tgttcttgaa aataaagttg aaaaaagtgt atctgtaatg
     7681 catggtaatt taaccgatgt aataaaagat aaagcggatg taatagttgc aaatataata
     7741 gctgatataa taaagatact tgcaaaagat gttcaaaact ttatgaaaga agatgctata
     7801 ttcatatctt caggtataat tttggataaa gtagaagaag ttaaagaaag cttaattgaa
     7861 aatggatttg aaatagttga agttcaaaaa ttaggtgaat ggtcagcaat agtttctaag
     7921 cttaaaaaat aagggatatg gtgacgtata atggatagat tttttgttga aaaaaataat
     7981 ataaatctac aagataaaac ttgcactata gaaggtgaag atgtaaaaca tatttcaaaa
     8041 gttttaagat gcaaattagg tgagaaactt gaaatatgtg acaagaataa taatgagtat
     8101 atttgtgaga taatgaatat agataagagt atagttaatt tagagatttt agaaaaagtt
     8161 gatataaata gagaatcaga acttaaagta aggctatacc agggacttcc aaaagctcca
     8221 aaaatggaaa tgatacttca aaaattaact gaagtgggtg tagaagaaat aatattagtt
     8281 caaactaaga gaagtgttgt taaagttgat gacaaaaaag aagataaaaa atttgaacgt
     8341 tgggaaagaa taatttatga agcagcaaag caaagcaaaa gaggtaagat accaaaatta
     8401 agaggtgttt taagttttaa agaagcttta gaagatatga aaaaaaataa tgtaaatatt
     8461 tgcccttatg aaaatgaaag aacagtttca ataaaacatg cactaaaaaa atgtgattct
     8521 aatatagata gtgttggaat ttttataggt ccagaaggtg gattttctga agaagaaata
     8581 gaacaaatac aaaaaaataa ttgtaatgtt gtatcattgg ggcctagaat attaagaact
     8641 gagacagctt ctgttgtagc atcaacaata gctttgtatg aactaagtga tttaggagga
     8701 gaaaaataaa ttgaaaaaag tagcttttta tacacttggg tgtaaagtta atcaatatga
     8761 aacagaagct atgctagaac tatttgagaa agatggttat gaacaagtca atagtgaaga
     8821 gtatgctgat gtttatgtta taaatacatg tacagttact catatgagtg atagaaaatc
     8881 tcgtcaatat ataagacgag ttaaaaagaa gaatccagat gcaataatag ccgttgtagg
     8941 atgttattct caggtatctc cggaagaaat tttggatata gaagaagtaa atttagtcat
     9001 gggtacaaat gatagaagaa agattgttga agaaattaaa aagataaatt caagtaagaa
     9061 agttagtaca gttgacgaca taatgaaagt taaagctttt gaagaaatag aaatcagcca
     9121 aactaatgga aagactagag catttatgaa aatacaagat ggatgtgaca gattttgtac
     9181 ttattgtatc attccttatg cccgaggacg cgttagaagt agagatattg atagtatagt
     9241 cgatgaagtg aaaaaattgg ctaataatgg ctataaagag gttgtattaa caggaataca
     9301 tgttgcctct tatggaaaag atttaaagga tagggatatt aaacttcttg atgtaataaa
     9361 acaaataaac cagatagaaa aaatagaaag aattaggctt agttctgttg aaccaatttt
     9421 atttacagat gaatttgtaa atgaagtttt aaagatggat aaagtttgtc ctcattacca
     9481 tttatcactt caaagtggat gtgatgaaac tctaaaaaga atgaatagac gatacacaac
     9541 tttagagtac aaaactattg tagatagatt gagaagtaaa atgccagatg tggcaataac
     9601 tacagatgta atagtaggtt ttcctggaga gacaaatgaa gagtttaaaa aaacgtacga
     9661 gtttttaaaa gaaatagagc tttcacaaat gcatattttt aaatattcac caagaaaagg
     9721 aactccagct gcaactatgg aaaatcaagt tgaccctcaa atgaaacact ttagaagtga
     9781 acaactttta aatttaagta aagtaaattt taataaattt gcaactaaat ttatcggccg
     9841 tgagttggat gttttgtttg aacaaaatat agaaggcaat aaatatgagg gattaacttc
     9901 aaattatata agagttgttg ttgaaagtga caaaaatata caaggtcaga tattaaaagt
     9961 gaaaataaat gatgtaaaag atgaatatgt agaaggaatt ttgctataac atgtagaaat
    10021 tataatattt ataagatagg aggtggaaag aatggattgt attttttgta aaatagcgaa
    10081 cggagaaata ccttcaacta aagtttatga agatgataga gtcttagctt ttaatgactt
    10141 gaacccagtt gctccatatc atatattagt tgtacctaaa aaacattatg atagtttaat
    10201 tgatattcct gataaagaga tggacattgt gtcacatatt catgttgtta ttaataaaat
    10261 agctaaagag aaaggttttg accaaactgg attcagagtc ataaataatt gtggaagtga
    10321 tggagggcaa gaagttaaac atttgcacta tcatatttta gctggtaaaa agcttcctaa
    10381 ttacgaagcg ggacaaaact aaactaaatt attgaaaaat tgagttcctt cttgataaaa
    10441 atacttgata aaagtaaaaa tttagtttat aataaacaaa gtgttaggtt gtaatgcata
    10501 ttatgcaaaa agaacatagt tacagtccca gcataattgc gctattggag ggagggaaaa
    10561 agaaatgtca gaagtaagag ttagagagaa tgaaacatta gacagcgctt taagaagatt
    10621 caagcgtcaa tgtgcaatgt ctggcatcat gtcagaagtg agaaaaagag agcactatga
    10681 taagccaagc gttaagcgta agaaaaaagc tgaagcagca agaagaaaaa acgctaagaa
    10741 atagtagtaa ataagaataa agaggtgaag gggatgtccc ttaagcaaaa gttacaagag
    10801 gatttgaaat cttcgatgaa aaacaaagat acagtgagaa aatctgtagt aactttaata
    10861 agagcttcta taaagcagta tgaggttgat aatagggtcg agcttgatga agatggaatt
    10921 atcgatgtaa tagcaaaaca attaaagcaa cgtagagatg cattagttga atttgaaaaa
    10981 gctggaagag aagacttaat aaaagaaaca gaaggtgaaa ttgaagtttt aaaagagtac
    11041 ctacctcaac aattaagcga agaagagtta gaagaaattg taaaatctac tatatctgaa
    11101 gtaggggcaa cttctatgaa ggatatggga aaaataatgt cagttatcca accaaaagtt
    11161 aaaggaagag ctgacggaaa acttataaat aagttggtaa aacaaaactt acaatagatt
    11221 gatatagcct agagagaaat ctctaggctt tttatagttt ttgggaataa cgagtcatat
    11281 ttgagcatat ctataatcaa agaaaatttt gaaagaaggg gaaaaatttg attatatcag
    11341 taaaagagat taggaatttt ttgataaaca tatttatacc actaataata ggttacttaa
    11401 gtagcatctt aacaatgatt atcagtggaa cagatatttc aacctattat ttacaactca
    11461 caaagccaag ctttgcacca ccagctttta tctttcctat agtttggact atattgtaca
    11521 tattgatggg tatatcttca tacctaatat taagaaaagg ttacaactta cctaaagtaa
    11581 gagatgcaat tttttattat ggattacaac ttgtattaaa ctttatttgg agtatactat
    11641 tttttggatt tggacttaga tttacagctt tgatagatat aataataatg attttggttt
    11701 ctataataat gatttcgaag ttttctaaaa tagataagag atctggatac ataaatttga
    11761 tttatttaat gtggcttgtt tatgcaggat ttttgaacta ttttatatgg tttattaata
    11821 aatagaatgt aaagattttg taaattaccc tgtactttta ataatatcat aacaaacgaa
    11881 aaataaatag actattaaaa ataatcattt tcatatacaa taatattaat ttaaatacaa
    11941 taaatttata aaaattgtat ttaaagatta acatattaaa gtattagaat tggaaaggct
    12001 tgaagggaga ggtttttatg caaataatag tatctgggag acaaatgaaa ttaactgacg
    12061 gaataaaagg atacgtagat ggaaaattaa gtaggttaga aaaatacctt gatcctgaat
    12121 cagaagtgaa agtaacagta agtgcgaaaa aggatagaca aaaagtggag gttacaataa
    12181 ttcctattaa cggtcaaata ataagagcag aagatgtaga agatgactta tatgcagcaa
    12241 ttgatattgt ttgtgacaaa ttaagtagac aagttgtcaa atataaaact aaagttaaag
    12301 acaaagttca aaataataag agcataagat ttgaaaatct tgactttata gataatagca
    12361 gtgaatttga tgattatgat tatgatgatg aagaagatga aaatatagta atagaaagaa
    12421 gaaagaaatt taatgtaaaa cctatgagtt ctgaggaggc gatacttcaa atggaactag
    12481 taggacacaa tttctatatg tttagaaacc aagataattt tgaaataaac atagtgtata
    12541 agcgtaaagc tggtggatat ggtttaatag aacaagatta ataaaaattg tatacgttta
    12601 gattttatat aagaaaaaat aaaaaggtaa tcatgttgat tttttatcaa tatgattacc
//
LOCUS       DAGSWI010000121        12577 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.121, whole genome shotgun sequence.
ACCESSION   DAGSWI010000121 DAGSWI010000000
VERSION     DAGSWI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.121"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(86..358)
                     /locus_tag="MJF80_003012"
     CDS             complement(86..358)
                     /locus_tag="MJF80_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281652.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGNHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(472..1344)
                     /locus_tag="MJF80_003013"
     CDS             complement(472..1344)
                     /locus_tag="MJF80_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBZ0281653.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(1640..4747)
                     /locus_tag="MJF80_003014"
     CDS             complement(1640..4747)
                     /locus_tag="MJF80_003014"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBZ0281654.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(4840..5072)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5247..5774)
                     /locus_tag="MJF80_003015"
     CDS             complement(5247..5774)
                     /locus_tag="MJF80_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBZ0281655.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(5809..6594)
                     /locus_tag="MJF80_003016"
     CDS             complement(5809..6594)
                     /locus_tag="MJF80_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBZ0281656.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(6604..6861)
                     /locus_tag="MJF80_003017"
     CDS             complement(6604..6861)
                     /locus_tag="MJF80_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBZ0281657.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(6880..7338)
                     /locus_tag="MJF80_003018"
     CDS             complement(6880..7338)
                     /locus_tag="MJF80_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBZ0281658.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(7378..8082)
                     /locus_tag="MJF80_003019"
     CDS             complement(7378..8082)
                     /locus_tag="MJF80_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBZ0281659.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(8092..8727)
                     /locus_tag="MJF80_003020"
     CDS             complement(8092..8727)
                     /locus_tag="MJF80_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281660.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFKKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            9108..10148
                     /locus_tag="MJF80_003021"
     CDS             9108..10148
                     /locus_tag="MJF80_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBZ0281661.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(10636..11991)
                     /locus_tag="MJF80_003022"
     CDS             complement(10636..11991)
                     /locus_tag="MJF80_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBZ0281662.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(12017..12286)
                     /locus_tag="MJF80_003023"
     CDS             complement(12017..12286)
                     /locus_tag="MJF80_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBZ0281663.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
BASE COUNT         3878 a         2283 c         1374 g         5042 t
ORIGIN      
        1 aataattttt acttaaaaca ttcaattttt atttaaacct tcatggaatc acaaaataaa
       61 aatctcttat aaatttaaaa tgtttttatt tatgttcatg tgtatgccca tctgaatgtt
      121 catgaccatg tgtatgtgta tgctcatggc tatgctcatg attacctgaa aaaccatcat
      181 catgattatg gtcgtgacct acaccattta catgctcatg ttcatgccca tgactgtgag
      241 aatgttgatg tgaatgttca tggttatctc ctccacagca tccttctcct ccagtcccac
      301 aacacccact tgtatgtgga ttatgatgat ttcttactac aaatctctct cttaacataa
      361 ttcttatacc tcctagtata ttattgagta cttcatgtac cccattaact acagtataca
      421 tctaactagg tataatctca atacatatgg cattttttaa catatataat cttaaagaaa
      481 tttatcctta aaacctccac cttttacttc ttcaacgtta ttgacagaaa taaaagcttc
      541 actatccata tcataaatta aatctttaaa tttacctatt tcatttgcag atattataca
      601 ataaatcatc tttttcttat tttgtgtata tgcaccttct gcctccaaaa aagtgactcc
      661 tctacccatt ttattcatta tcactttgga tatttcatca tatttatttg atattaaaag
      721 tatagatttt tttctatcaa agcaatcttt tcctatatcc atagtaaaag aaattatata
      781 cattgtaatt aaagtgtaca tcaccaattt agctccaaat aaaaaagctc ctgcacatac
      841 tacaaaaaaa ttaataaata aaaatgtatt tttcataggt atatcatact ttcttttcat
      901 tatggctgct attatatcaa gtcctccagc agtagctctt gctttaaaag ttaaccccat
      961 acctatacca gttaatgcac caccataaat acactgtagc atagtatcat ctattgggaa
     1021 atatttagca atatcttgtg ttagccccag tgctagagaa aatagtaaca tattgacaaa
     1081 acttattagc aaaaatttat tatcaaaata ctttctagct ataataaata ttggtatgtt
     1141 catcaagaaa cttgatattc cctgatttat accaaataat ttgtataata taacacaaat
     1201 tccagctact cctccactta ataacttggc tggtgctaga tataaattta ttcctaaggt
     1261 tgatagaatc atacccaata taataagaca atattgtaaa aatatgttat tatcaatact
     1321 tagcttttta ctttcaccat acatcaaaac gcaccccttt gcataataaa taaaccaccc
     1381 tgcagaacac acgataaatt tacaaataaa tttatcagaa gtagcttact aaaaatatta
     1441 ttcatcctca cttaaacgtt tattcattaa tattctatca catgacataa ataatttata
     1501 tgatttatat agatttattt attgtaaatt ttggaatatt aaaatatttt attgaataga
     1561 taattatatt acataaaaaa tccctataga aatctataaa ctaatttcta tagggatttt
     1621 ggttaactaa ctattctctt tattttttta taacttttat acctgtcata tggtcattta
     1681 agttttgttc ctctaaagat ttatcatcta ctctgtttat ttctaaagct aaagtttcag
     1741 acttgatata ttcattaaaa tggtcaacag ccttagcaat ttcatcatca ccacagtatt
     1801 ctattactat attatctaat acttcaaagt tattagattt tcttaattgc tgtactttag
     1861 atataaattc tctagcataa ccttcattaa tcaactcttc agttagctta gtatctagta
     1921 taacaaaaag attgttttca acagatacat taaatccttc cttagcagtt atacctatta
     1981 atactaaatc cttattaaat tcaaaagctt caccatctaa atcaactgtt aaagtctcac
     2041 ctttttcaag tttattagca gtttcatgag catttaattt acttaatgct ccaacgaata
     2101 aattcatctt agaacctaat aatggccctg cttccttaaa gtttggtttt aaactaaagt
     2161 tcatatactc atctaaatcc ttagcaaata ttacctcttt aacattaagc tcttctttta
     2221 ttaaatcaac tacatcactt attgtatctt caaacttacc atctactaac actttttgta
     2281 atggttgacg aacttttatt ctttctactt ctcttgaagc tctacctaaa gtaactaagt
     2341 ttttagctaa atccattttt tcttctaatt tagtatctac taactcttca ttacatttag
     2401 gatattcagc taaatgaaca gatacttctc ctgttaagtt tctatacatt tcttctgaca
     2461 tatatggtgc aaatggagct atagctctac aaagaccatg taacaattca taagttgtat
     2521 tgtacactgc ttttttatct tctgttaatt cagttgccca gaatcttctt cttgaacgtc
     2581 ttatatacca gttagataaa tcatcattta taaaatcttg tatcattctt aaagttttat
     2641 ttaactcaaa tatagctaaa ttttcttcta catctttcat taaattattg aacttagata
     2701 atatccatct atcaagttct ggtctatctt tatattctac aaaaaattca gttggattta
     2761 tatcatctgt gtttgcatat aaagtaaaga agttatatac attcttcata gtacctataa
     2821 atttactttg gatttctttt aatccatcca tatcaaattt agttggagtc catggtggag
     2881 aaacatatag taaataccat cttaatgcat ctgctccata ttggtcaaat aattccattg
     2941 gatttactgt atttcctctt gatttactca ttttcttacc atctttatca agtacaaggt
     3001 ctggaactaa aacactctta tatggtgctt ttcctaatac aaatgttgat actgcaagta
     3061 atgagtagaa ccaacctcta gtctggtcta taccttctga tatatagtca gctgggaaca
     3121 attggtcaaa gttttcctta ttttcaaatg gatagtggtg ttgagcaaat ggcatagccc
     3181 cactatcaaa ccaacagtct ataacctctg ttactcttgt cattggtttt ccacattttt
     3241 cacattttaa gtgtatatca tctacatatg gtctatgaag ttcaactgtt tctggattta
     3301 catcttctat agctttttca gctaattcag ctcttgaacc aactgaatct gtatgaccac
     3361 attcacatct ccatatagga agtggagttc cccaatatct agttcttgat attgcccagt
     3421 catttagatt ttctaaccag tttccaaatc tcttctctcc tacaaaactt gggaaccatt
     3481 ttacaccatt attattttct attaatttat ctttaagttt agtcatctct atataccagc
     3541 ttggttttgc atagtatact aatggagttt gacatctcca gcagtgtgga taattatgaa
     3601 ctactttttc tttgctatat aatttatttt cagaagctag ccattttatt atttcaacat
     3661 ctagcccatc ttccattaca aatctacctt cccaaggagt agatgtaaat ttaccagttt
     3721 catctactgg ttgaataact ggtaaatcat atcttttacc tgtgttgtag tcatcttcac
     3781 caaatgctgg tgctgtatga actataccag ttccatcttc agtagttaca taatctccta
     3841 ctgtaataaa aaatgctttt ttgtcaactt ctacaaaagg cattaattgt tcatattcta
     3901 aaccttctag gtctttacct ttcatttctt ctaaaacttc ataatcttca cctagtactt
     3961 tatcagctaa aggtttagat acataatata cctcatcacc ttttttaact tttaaataat
     4021 ctacatcagc atttactgtt aatgaaacgt ttgatggtaa agtccatgga gttgttgtcc
     4081 atgctaagaa gtattcatca gcatcttttt tcttaaattt agctataact gtattagttt
     4141 ttacttcttt atatccttgt gcaacctcat gactagcaag accagttcca catcttgggc
     4201 aataaggaag tattttatgt ccttcatata tatatccttc tttgttgaat ttgtttagta
     4261 tccaccatac agactctata tagttattat ctaaagttat atatggatta tctaaatcaa
     4321 cttcataagc cattctctca gtcattattc tccattgttt ttcaaatgag aaaactgact
     4381 ctctacattt ttggttaaat tcagctattc catattcttc tatttgttgc ttgctagtaa
     4441 gacctaactc tttctctact tgtatttcaa ctggtaatcc atgtgtatcc caaccagctt
     4501 ttctctttac ttgataacca gacattgttt tatatctaca aactgaatct ttaagcgttc
     4561 tagataaaac atggtgtact ccaggatttc cattagctgt tggaggtcct tcatagaata
     4621 cgaaacttgg gtcatctttc cctttttcta aagttttttc aagtatattt atgtctttcc
     4681 aataatcaaa aacttgtgct tctgcttgtt ttacagaact gtctaccaat ggcttaaatt
     4741 tcgccatgta aatcaccctt tcctactaaa tattataatt ccaaaatttt ttattctttc
     4801 acataaattt aaatactaaa aaactcgccc cctttaaacc aaagggacga gttataaatc
     4861 gcgttaccac cctaattcta tatgtaaact aaatactagc tacatataac actctaatag
     4921 tataacggta ctaaccggca cagcttagta gaatttaatc gttcagcctg cagctcaaga
     4981 gtgattttta tctttataaa ttcattggtt ctcagcatac ccaattctct gtaaaattat
     5041 ctaaagataa atgtctctat caacgctttt aaaaatttat ctgtgtttat atgaaattct
     5101 ataacaaata atttgaatag tcaatatatt tttgaaaaat ataataattt ctatttttct
     5161 tacattttta tcctattttt aatctttgtt atagatttct caaactcaca caattattct
     5221 acataaaaaa taaacaaatt acaagcttat tctaaagttg tagcagcttc atcattacta
     5281 taattataca ctgttgttcc ttcaaatgcg tttacacatt tttcatctac atcataaaaa
     5341 atctcatcaa cacttcttat ttcatcttct aacagtgatt taaatttatt tctaaatatc
     5401 ttaaattctt tgactaaagc atcatactct tttcttatct ctataacacg gttattagct
     5461 tgttctataa tttgtctagc ttttaaatca gcttcttcaa caattatctt agccttttta
     5521 ttagccgcgc tacaagtgtc ttctgctgca ctttgagcag ttattaaagt agcttttagt
     5581 gtttcttcaa tattttcata cttgtttact tggtcttgat ataaccctaa tttttcttta
     5641 agagctgtat tttccctaca tagagattca taatcttcct tgatgatatc taaaaattca
     5701 tcaacctctt catctctata ccctcttaaa ccttttttaa actctttatt ttctatctca
     5761 attggagtta gcattacatt tcctcccagc tataatttaa attagtcctt atacaattaa
     5821 tcttgcttga acttttattt ttcctttttt agtaatatct cctatttgtg ttaccttagc
     5881 tcttcctttg cctctaatag aaatcaaatc atcatttttt actattttag aagttgaagt
     5941 tatcttttca taattgacat ggactttttc tccatttatg tattttgcac tatcttgtct
     6001 agatatattg taaataccac ttatcacaca atctaaccta tctgaagata ctgtaaatga
     6061 tacttcttta tattttgaag aactattaac aatattttct ctggaaattt cctttacaat
     6121 tacattattt cttgaaacct tttctaagtt tacaattata aaatcgcaaa tatcactact
     6181 tacaactact tgacaaaagc tatcatgtat aattatatct ccaatttttt ctctttttat
     6241 acctagaccc agtattgacc ctaagtaatc tttatgagat atatttttaa atttgaaatt
     6301 cccttctatt tgtaaaaatc taagagtatc ttctatatct tcatattcca tgtaaaatgg
     6361 ataaataaat acagtgcttc tttcagcttg ttcataacct ccatccacac tgtattttat
     6421 ttcatttgta gaatttaata ttgctacagc atttttcact tcatatggat taagaaactc
     6481 cgtagactta acatcatagt tttttataca actattagcc ttgtctatca ctttaaacat
     6541 tttatttttc aagtctatat cctttatgtg atttgttagt tttaatttat ccatcctatc
     6601 accttagaat ataaccaata ctaattgtct taggaacatc aataatacta ttgcaattaa
     6661 aggagaaaaa tctataggtc catttgtata tttatacatt atagacctta ttggcccttc
     6721 tattggctct gtaagatttt ccaaaatatc aaaaatttta ctttgtcttc catttggaaa
     6781 ccaagtcatc aagcttttta taactatcat ccatgctatt atatctaaaa gataataaag
     6841 tgcaattctt attgttccca tatattaacc gcctttttac tatttatttt gccaagggaa
     6901 gaatgcttta ctttctaatt cttttttcat atttgcatct atatccacat tattaggagc
     6961 tagtataaat attgatttag aaaccttttg tatacttcca tctaagacat aaactgcccc
     7021 attcataaag ttaaatatag atttacgaac ttcagaatta gtcaatcctt ccaaattcac
     7081 tataactgcc cttctttgtt ttagatggtc tgctatagtt gcagcttcat cataaacctt
     7141 aggttcaact attacaactt tcatttggct agttgtatgt aaatttacaa ttttatttgc
     7201 ttttgcagta ctaaagccac tgttcatttc ttcttcttga actatatcgt ccatgccact
     7261 ttcatattca tcttcaatgt aatcttcttc ttcatcaact atccaattct taaatttaga
     7321 tattattcca tttgacatta ttaacttcct ccttaaaatt tattcaaatt tgagtaatta
     7381 ttttttattg tagtttcttt ctccaaagat tgatgttcca actctaacaa tggtagcccc
     7441 ttcctctata gcaatttcat aatcgtgact cattcccatt gacaaaacat ccatccctac
     7501 attagggatg tttagttctt ttatttgttt tgataaatct ttaagacctt taaatacacc
     7561 tcttatctca tcttcatctt ctataaaagg tgccattgtc attagaccct ttatttttat
     7621 atttttatat ttttctgaca cttctttgac aaagtctaca acttcttctc tatcaagtcc
     7681 atgcttagac tcttctttag atatattcac ttgtactaag caatctattt ctctgtttat
     7741 ttgttctgca cgtttttgaa tttcttcgca taatgcaatt ctatctaaag agtgtatcat
     7801 acaaacttta tcgattatgt acttaacttt attggtttgc aaagttccta tcaggtgcca
     7861 gtttaattta tcacctataa tatcatactt tctagctaat tcttggggtt tattttcacc
     7921 aacagaagtg attccttccc tcattgcctc attaactaag tctacatcaa cagtctttgt
     7981 gactgccaaa agggtaatct ctttgctgtc tctatctact ttttttgcag ctttatcaat
     8041 tttgtttctt atctcagaca aattatcttt aatagaaccc attattatca cctactttat
     8101 ccttatattt gtgtttatac tgttaggtct taaaattatt ttgtcatata gattaacact
     8161 tgtaactcca tttatgtcat ttttataata atccacaaat acagaagatt cattctcata
     8221 agttattcct tctaatatga caaattttac actttgtgat tgttcatcaa ttacatatac
     8281 accacgttta ttgtcttttg tttttataga agttttaggt atttttaaac attccatctg
     8341 attatatatt atatcaaatt cttcgacact tgtatcctga tttgcaacaa tttggttact
     8401 tatttttaat tttaccatta ttttattatt attttcatat attttgtaaa cttttgcatc
     8461 taatttttgc tcatgatatt caattattac attttgattt tttttaaagt tattagaatc
     8521 ttctttactg atgtatgtag atagataatt actctggtca ttggcatctt tttctatagt
     8581 tccttttaca tgaatctttg catcaacttt ttcatttttt aacaccaacg tatctacatt
     8641 tttgctaatt atcattacta atatattaaa tactaaatat ataagaatac caagtatcac
     8701 taatctggta tattttacct ttttcaaaga ctctccccct ataaaattac tttcattata
     8761 agtacttctt ctaaaaaatg tttattcctt ttttataagc taatatttta cgttctaata
     8821 attatttttt ccatattaaa tttatttttt atcaatcttc ctatacaaga ctgcattatt
     8881 tatatataat tgtattataa agtataagtt taatttacat tttatacgtt aagcttactt
     8941 tatatgtttt tatagttatt tttattttta gatgcaatat aaaaccaatt aagatttaaa
     9001 ataacttttg ttatgttgac atttatttag gtaatcattt atgaattagg ataacgttat
     9061 cataacaaaa gtttatgtcc taaaaaaaat acaaggagga ttttcttatg tcagaaattt
     9121 tattaggaac aggtttactt ttattggttc tatgttgttt cacaattttt agttacaagg
     9181 ccccatatgg tatgaaggct atgggagctc tagcaaacgc tgcatgtgct actttcttag
     9241 tagaggcatt caacctttct ttacttggtg atgttttagg tatggaattt tttagaagtg
     9301 ttggagctgc taatggtagt cttggtggag ttgctgctgc tatacttgtg cctttagcct
     9361 taggtgtatc tccagtttat gcagttttaa taggattatc ttgtttagat ttttctatat
     9421 tatctggatt cgttgctgga tactttgttt ctttcttaat taagtttata gagaaaaaag
     9481 tgccacctgg agtggattta gtggttgtaa ttcttctatg tgcaccacta tctcgtctta
     9541 tagcatcaaa tgtagaccca ttagttaata gtacactact acaaattgga caagttttga
     9601 tagaagctac caacattagc cctataataa tgggaattat actaggaggt ctaataacag
     9661 tagttggaac gtctccactt agttcaatgg ctcttacatc tataattggt cttactggtc
     9721 ttcctatggc aataggagca ttaggtgctt ttggttcagc ttttatgaac tcaacactat
     9781 ttgcaaaatt gaaattaggt tctaaaaaag atattatatc tgttgctatt gaaccattaa
     9841 cccaatcaga cataatttca gctaatccaa tacctatata ttctactaat ttctttggag
     9901 gagccttttc tggtatcatt gtagccttga tgggtcttgt aaacatgaca ccaggtacag
     9961 ctacacctgt ggctggtttt gctataatgt ttgcttataa tccagttcaa caagttctta
    10021 taacagctgg tttatgtgct ttattcaatg taattgcagg atttttaggt tcaaaaatat
    10081 tcagtacctt taaagttctt actgctggtg aaatacgtgg tactgatgta gaagaagaaa
    10141 ttgcttaaaa aacaataaga tttataaatt tatatctcaa gtagtagagt tttatattgt
    10201 aggcaatttt attaattgca aacgttttat tttttattta tgtttatgat tattatgtgt
    10261 gttgaatatt tagataaatt tttatttatt atttttattt agatttttat ttattatttt
    10321 tatttagatt tttgtttagt tttttatttg gttgatatat taggaataaa aattagtata
    10381 gtttattact agttagatag cataactttt tagatacata aaaaatttta tattaattag
    10441 atattattaa aataaaagcg atacatgaat ttttaaatgt ccatgtatcg ctttattttt
    10501 gccataatct taacaactaa tttttaaagt ttattatctt aacaaagttc attatcttat
    10561 ataatattta aatgcataga cttctttaat ttaaccatct aaatatttaa ttaaatatta
    10621 ttatatgtaa tataactaat ttttaaatga atgtatagga gctggaatcc ttcctcctct
    10681 ttgagtaaat aattcagatg aatttgtatt tataggcatt actggtgctc gtcctaaaag
    10741 tcctccaaat tctaccatat cacctacatc acatcctggg gctggtataa ttctaacggc
    10801 tgtagtctta ttattaatca tacctattgc tgcctcatca gctatcatgg ctccaagcgt
    10861 acctgctgta gtatttcctg gcacagctat catatcaaga cctacagaac aaatagcagt
    10921 cattgcttca agcttatcta tacttaaagc acctttaata acagcatcaa tcatacctgc
    10981 atcttcagat actggtataa acgctccact aagtcctcca acatgtgaac atgccattac
    11041 tccacctttt ttaacagcat catttaaaag tgctaatgct gctgtagtcc catgagtacc
    11101 aacaacttca agacccattt cctctaatat atttgctact gaatcaccaa tagcaggtgt
    11161 aggagctagt gataagtcta ttatcccaaa tggaacatcc aatctactag aagcttcctt
    11221 tgccacaagt tgtccaactc ttgtaatttt aaatgcagtc tttttaactg tttctgaaac
    11281 aacatcaaaa ggctctccct ttactttctc taaagctctc ttaacaacac cagggccact
    11341 tacaccaaca tttataattc tatcagcctc accaacacca tggaaagctc ctgccataaa
    11401 tggattatct tctactgcat tacaaaatac tactaatttt gcacaaccaa aaccttttgc
    11461 atcttttgtt aattctgcag tttcttttat gatttctccc atttctttca ctgcatccat
    11521 atttatacca catcttgtag aacctacatt tactgaagca catactttat tagtacttgc
    11581 tagagctttt ggaattgact taattagtat cttatcacct tttgtatatc ccttttgaac
    11641 taatgctgag aatccaccaa taaaatctat acctaaatcc tctgcagcct tatccaatgt
    11701 ctgtgcaaac tttacatagt cattttcatc tgttgcacct gcaataatgg aaattggagt
    11761 tactgatacc cttttattta caattgggat accaaattca cgctctatat cttttccaac
    11821 cttcaccaaa tctttagcag aactcataat cttattgtat attttttctc tagctttatc
    11881 tccatcaggg tctatacaat ctaataaaga aatccccata gttatagttc ttatatccaa
    11941 ctgttcttca tctatcatct ttattgtttc aagtatattt cctacattca ctattatcac
    12001 ctccccttac tgtctactac agattatgca ttgaattaaa tatttcttct cgctgaattt
    12061 ttacatccac tgataatttc ttacctcttt caaccaagtc ctcaaccgtt ttctcaaaag
    12121 aatttaatga tttctctaaa tcaactatca taatcatagt aaaataatcc tgtaatattt
    12181 tttgtgatat atctactatg ttgatatttt gcttgtataa ttcatcacta attcctgcca
    12241 ctatacctgg tttatccttt ccaattactg ttaaaattgc tttcataata atatcctccc
    12301 ttattttttt ataaatctta tatttttaca aattgaacta tgtgttaaca tatatttcat
    12361 ttatttgtta gtaaaccact ttttatgtat atatttaata tattttaata cctatacatt
    12421 catttgtcaa ttttttttca atattctatt atttttatac tatttagaaa attcatatat
    12481 tatatatgtt ttctcaacaa caaatacagt tttcataata atgaaaggta ttacttagca
    12541 atctcatgat tttctaaata atacctttct aaaaatt
//
LOCUS       DAGSWI010000122        12516 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.122, whole genome shotgun sequence.
ACCESSION   DAGSWI010000122 DAGSWI010000000
VERSION     DAGSWI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.122"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            127..777
                     /locus_tag="MJF80_003024"
     CDS             127..777
                     /locus_tag="MJF80_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBZ0281664.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(917..2293)
                     /locus_tag="MJF80_003025"
     CDS             complement(917..2293)
                     /locus_tag="MJF80_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBZ0281665.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2309..4222)
                     /locus_tag="MJF80_003026"
     CDS             complement(2309..4222)
                     /locus_tag="MJF80_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBZ0281666.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4358..4990
                     /locus_tag="MJF80_003027"
     CDS             4358..4990
                     /locus_tag="MJF80_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBZ0281667.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5129..5668
                     /locus_tag="MJF80_003028"
     CDS             5129..5668
                     /locus_tag="MJF80_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBZ0281668.1"
                     /translation="MVFVEELWKKAIKFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5844..6014)
                     /locus_tag="MJF80_003029"
     CDS             complement(5844..6014)
                     /locus_tag="MJF80_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBZ0281669.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6299..7366
                     /locus_tag="MJF80_003030"
     CDS             6299..7366
                     /locus_tag="MJF80_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBZ0281670.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7536..9437
                     /gene="cooS"
                     /locus_tag="MJF80_003031"
     CDS             7536..9437
                     /gene="cooS"
                     /locus_tag="MJF80_003031"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBZ0281671.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9430..9903
                     /locus_tag="MJF80_003032"
     CDS             9430..9903
                     /locus_tag="MJF80_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBZ0281672.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            9878..11104
                     /locus_tag="MJF80_003033"
     CDS             9878..11104
                     /locus_tag="MJF80_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBZ0281673.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFNIKENGEFCYSV"
     gene            complement(11243..12430)
                     /locus_tag="MJF80_003034"
     CDS             complement(11243..12430)
                     /locus_tag="MJF80_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBZ0281674.1"
                     /translation="MNITKNFLKDYLERFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNTRIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
BASE COUNT         4716 a         1594 c         2003 g         4203 t
ORIGIN      
        1 ctgcgtgagt agaatgaatt tgtcaagttt aatttagttg taattttatc ttttattaaa
       61 aataatataa attaaaacat ataaataaaa tattttatac aatatattgt aaaggaggta
      121 actttgatga aagcagaaaa aacaagtata tatccaatta ttaaaaataa tttaaacaaa
      181 atatcaattg ttgaatataa aaagggtcaa agatttgtag catcagataa agacttacaa
      241 gaagtgttct ttataataga gggagttgca ttagtggagt gtactactag aggaggaaat
      301 aaatttcttg ttgacattgt acctgaaaat gagtttatag gcaaaattag ttacatatat
      361 gaacataatt tgaaatgtga tatatttgca aagactaata taaaattatt tcgatttgaa
      421 aaaaatgtat ttgaagagtt ttatctcaaa ccagatttta ttgcactatt tcacagaaaa
      481 tgtacaagaa gaatatatga attatataaa accagaatgg taagagaact attttcttgc
      541 acagaagtta ttgcctattg tatatcaaat aaacaagaaa ataatgtctg caatataaaa
      601 gctattcgta atttcagtga aatctattca attagtagaa aaagtagata ccattctctg
      661 ctaaagttaa ctgaaaagga gatagtaaaa aaaatgggta atacatatga aattttaaac
      721 tatgatgaat tacataaatt ggcatttgaa gtaaaagaat ttttggaaga tgagtaaaaa
      781 ttgttactta atcaaaataa agtataataa tttctgtaaa aaatagaaat agtctcactg
      841 aaaatcaaaa atactttagt tattttcaat gagactgttt agaaatattg tatacttata
      901 atgtatagta taattattaa tcaatttcct ttactatatt aatagcccat tttttagata
      961 atgctctagg aacaccaaat ataaacttaa ccacttcttc actataagtg agcaaaacga
     1021 gtacaaatat aggtgcacct ttaaatgcag ctataaaagt tagaggaata gaagctaacc
     1081 acatacatcc taattctaaa aatagagcat atttagtatc tcctccactt cttaaaatac
     1141 caattataac caatgtatta aaagatttaa gagccattag tattcccatt ataaagaata
     1201 tcttagttat atcagaagct aagctatcag aaacactaaa catttttaat aataatggta
     1261 tatttaaaat taaaataatt ccaagtatta aaccagctga aaatacaagt atagaaaact
     1321 ttttagcata ttcaatggct cttttaatat ggtcagctcc aagttcattt ccaagcataa
     1381 tagatgcacc agatgcaata catacagctg tcattatgaa gaaattacct gtactggttg
     1441 ctatctggct agctgctata gcagaagtac cagctcttgc atatgcaaca gaatacagga
     1501 cagttccaat tgcccataca gagtcattta caaatatagg aaagcttttt gatgaaaaag
     1561 attttataaa aattttatca atagctttta gattttttag accaaacttt aatatatagt
     1621 cttttttata aaggtaaaca taagtaatca ttaaaattaa ttcacatatt ctagcaataa
     1681 cagtagctag agcagcacct tttacaccca atgcaggaag accgaagtta ccaaatataa
     1741 atccataatt taaaataaca tttgttacaa gtgcaaatgc actacaaatc atacctaact
     1801 taggatttct aacacctctt gaaccagtac taaatacagt acttacagca attaatggat
     1861 aactaaatac tgcaatacta aagtaatcta tacaaagttt taccacctca gaatcatacg
     1921 aaaatatgtg tataattggc tttggtgtta gtaaagcagg aataacaaat acaaaactta
     1981 aaacaattgc caatacacaa gtaagacctg tgacttttct tatattactc acatccttct
     2041 ttccataaaa ctgtgaaata aaaactccag cacctcctgt gataccagat aaggacatat
     2101 taaaaagaaa gaaaaattgg tttgcaactc caactgaagc tacagatgtt tcccccaaac
     2161 tacttatcat aattgtatct attacattta cagaagtaga aattaagttc tgtatgataa
     2221 taggtataca taaagataga agtattttat aaaatctttt attttcgtct tttaaagaaa
     2281 atagttcttg caaataatcc tctccttttt acataaattc ttcccactca tcatatagag
     2341 aggcaaggtt attttctaaa ttaatttttt cttggctaac ttctttagct ttatctggat
     2401 tagagtatac ttcttcttga cataaaagta tatctaattt ttcaatttca atctctaact
     2461 tttcaatttc tttttctata ttttgtcttt ttattctatt tttcttttct atttctcttt
     2521 gttctctctc ttttcttttt tcatctttta tttgagttct agttttttct tctttttctt
     2581 ctataatact catctcttgg atttgccttt ttttatctat ataataatca taatttccca
     2641 gatattcagt aattccattt tcatctaaaa ctaaaatttt atcaacaaca gtatttagaa
     2701 agtatctatc atgtgatatt gtaaatatag tcccatcata attagttaat gcctcttcaa
     2761 ggacttcctt agaatcaata tctaggtgat tagtaggctc atctaataac aataagtttg
     2821 catttgatag tataagttta agtatggcaa ctctagctct ttctccacca ctcaatgtag
     2881 aaatcttctt aaaaacttct tcatctacaa ataaaaatgc ccctaacata tttctcaaag
     2941 tagtttgagt aagatgagta ttattttccc aaatctcatc aattattgtg ttatctaaat
     3001 taagagtttt ttgttcctga tggaagtagg aaacatgtac attagttcca aatttaatat
     3061 tgccactaag aggttgtagt tcattagtta ttattttaaa taatgtagat ttgccaacac
     3121 catttgcacc tataagtgca actttttccc ctctatatat atctaaatcc aagtctttaa
     3181 aaagtattct ttctccataa cccatagata catctctaat ttgaagaaca tcattaccac
     3241 tctggacaga aggcgtaaat tgtatacgag cttttttcct ataagcttct ggtttatcta
     3301 aaacgtctac cttgtctaat gctttttctt tacttcttgc acgctttagg tgtttttctc
     3361 taccataagc ttttagtctt tcaattgatt cttcttgctt ttttatttct ttttgttggt
     3421 cttcaaattt cttaagctct agttcttttt ctattttaga taactcaaca aatttagagt
     3481 agttaccatt atatacttta agttttttgt tgtgaacttc aaaaattcta tttacggatt
     3541 ggtctaagaa atatctatca tgagatatta acatgacagt ccctttatat tgttttaaaa
     3601 atacttctaa ccattctatt gcttcagagt ctaagtggtt agtaggttca tcaagtaaca
     3661 atagagttgg tttcttaaga agtaattttc ctaaaagaac tctagttttt tcaccaccag
     3721 aaagtatgct tattggctta tccatatcat tgtcactaaa tcctagtcct tttagaactc
     3781 ctttggcttc agatttatat ccatatccat ttaaatctga aaataattct agtttatgtg
     3841 aatattcatc catgattttt tctataattg gagagtttgt ttttgtactt tcttcagata
     3901 ttttaacctc caattctctt aaataggatt ccatttctat caaattttta aaaacttcta
     3961 gtacttcttc caaaatagta ttatccgaat aaaaattggt attttgttct aaatatccga
     4021 tttcacaatc tttagaagtg taaatatctc cactatcata tccataaatt cctgaaatta
     4081 ttttaaataa agtagttttt ccagtaccat taacaccgat tataccaatt ttatcgcctt
     4141 catttactgt aaaactaata ttttctaata tagaatctat accaaaactt ttatttaagt
     4201 tattacatga caaaacaatc ataaatttct acctccttga actatctaaa attataatgt
     4261 tttgatataa tataatcaag gttatatgta ttagttttga aaaagaaata ttttattgtg
     4321 aatcaaacaa aaaagaggac acttttagga ggtgaagatg ttgggaaata aaaatatatc
     4381 aatggcagtt ataagaaggc tcccaaaata tcatagatat cttggagact tattagatag
     4441 ggatatacaa agaatatctt ctaaagaatt gagtgatata atagggttta ccgcttctca
     4501 aataagacaa gatttaaaca actttggtgg atttggacaa caaggatatg gttataatgt
     4561 agaagctctt catactgaga taggtaaaat tcttgggttg gatcgaccat acaacgcagt
     4621 tcttgtagga gcaggtaact taggacaagc tatagccaat tatgcaggat ttagaaaagc
     4681 tggattcgag ataaaagctt tatttgatgc aaatcctaga atgataggtt taaagataag
     4741 agagtttgaa gtattagatt cagatacttt agaagacttt ataaaaaaca ataatataga
     4801 tattgctgta ttatgtatac ctaaaaatgg agcacaagaa gttattaata gagttgtaaa
     4861 agctggaatc aaaggtgtat ggaattttgc acctttagat ttagaagttc cgaaaggtgt
     4921 tatagttgaa aatgtaaact taacagaaag tttatttacc ttatcgtatt taatgaaaga
     4981 aggaaagtag acaacttaaa catattaata atgagcagtt gtgaagagta ttgagaaagt
     5041 cagaagattt aatattaaat cctctggctt tttgaaatat taggataaaa atttaaataa
     5101 tgtatggaaa taaacaatat taaggagagt ggtttttgtg gaggaattat ggaaaaaggc
     5161 aattaaattt catggtcatg aatgtccagg attagctata ggatttaaag cagcacttgc
     5221 agcaagagaa tatttaggat gtaaacagtc aggagatgag gaaattgtat gtatttctga
     5281 aaatgatgct tgtggaatag atggtataca agtcgtgcta agttgcacaa tgggtaaagg
     5341 aaatttacta cttaggatga ctgggaaatc agtatatcat ttctttaata gggaaaatgg
     5401 aaaatctata agaatatatg taaagtcaaa gccatttcaa atggatagag aaggatacaa
     5461 aaagtactta ttagaaagtg attttgaaga aatatttgag attacagata ctaagttaag
     5521 acttcctgaa aaagcaagaa tatttaaatc tcttaaatgt gaagtgtgtg gtgaatttgc
     5581 atctgagcaa tatataagga ttgaagaagg aaagaaagta tgtcttgatt gctttaataa
     5641 atatgaaaga ttttatgagg agtattaaaa ttataaatta gattaaagtt aaaagtatta
     5701 gatatgtgct aacagtattt taagatgaat taggtagcaa cataataaag tatataaata
     5761 atattaattg aatcatacat aattaaaaag aggctgatta gtatttataa aataaaatca
     5821 gtctctttat gaataacgaa ttattattct ggttgaggtg catctacagg acaagctcct
     5881 gcacaagaac cacagtctat acaagaacca gcatctataa catatgcgtc gtctccagct
     5941 gatatacagc taacagggca ttcagcctca caagcaccac agcttataca agcgtcagta
     6001 attttatatg ccattttgaa atcctccctt ttatatgtat tgtaattcaa taacattata
     6061 acaaatatta ttgaatataa caattaaatt aattctacaa caaaaacttt attatgatat
     6121 tcttgtttta aagttttaca ataaagttta atctgcttat atttttattt attttaaaga
     6181 agtttatttt aaaaataaaa ttaaatttaa aaagttatgt attgattata tttaaaaatg
     6241 ttatactcta aaaaagtata ttttatttaa aaatttatta atacaaagga gatttactat
     6301 gaaaaataaa ttagtttaca tatgtattat gagtctattg ctatttgtag taggatgttc
     6361 taataatcca aataaggaaa gtaccattaa aaaagactct aagaataacc aaagccaagt
     6421 taaagaagaa aaaagagaac ctgtgaaaaa agatataact ataagtttcg ctggtgatgt
     6481 aactatgggg aactataaag gttcatcata ttatggcagt tttgaccatg aatttaaaag
     6541 acaaggagaa gattacaatt attttcttaa aaatgtaaaa cctgtatttg aaaaagatga
     6601 cttaacagtg gttaatttag aaggtccact tactacagcg tcaaatgcaa aagaaaaaaa
     6661 atttgcattt aaaggaaagc ctggatatgt aaatatttta aaatctggaa gtgtagaggc
     6721 agtaagtatt gcaaataatc attcagaaga ttattttgaa gaaggaatga aagatacaca
     6781 gtttatattg gacgagaata aaataaatta ttttggatta gggaaagatg ctgttgtaga
     6841 tgtcaaagga attaaagtgg gtctattagg gtataatgga ttatcaactg aatataatga
     6901 aaaaaattta aaacaaatgg aagatgatat taagtcctta aaaaagaaat cggatgttgt
     6961 tgtagtgtac tttcattggg ggatagaatt agattattat ccagataaaa aacaaaaaga
     7021 ttttgctcat tatgctatag acaagggagc agatttagtt atggggtctc acccacatgt
     7081 gattcaagga atagaaaagt ataaaaataa gtatatagct tatagtctag ggaatttttg
     7141 ctttggagga aataagaatc cttctgatac agatagttat atatatcaac aaacctttac
     7201 tttttcagat aataaattaa cttcaattaa agagccaaat ataataccaa cttcaataac
     7261 atctagtaac tccagaaata attatcaacc aaaaatattg gatggaagtg agaaggatag
     7321 agttttaaat aaacttgaaa aaataagcaa agatttaaat aaataaaatg taaaaaaaaa
     7381 taaatatata tatttatttt aataatttat attttgttta gggtatggta aatcgtggta
     7441 ttaaataagt agattttaat agttaaatta tagtcaatta taataaagct atttaatcta
     7501 cttttttaat ataaaaatat aagggggaat ttgtaatggc ttgtaacact tgtaaaatgt
     7561 gcgaaagtgc agacaagaaa ttagaaagtt ttgtagcttc aaaggatgtt gaaacagcat
     7621 ttcatagaac agaagatcag aaagttaagt gtggttttgg acttcagggg gtttgttgta
     7681 gactctgttc aaatggacca tgtagagtaa ctcctaaatc accaagaggt atatgtggag
     7741 cagatgcaga cactatagta gcaagaaact ttttaagagc agtagcatca ggagcagctt
     7801 gttatttaca tgtggtagaa aatacagcta agaatttaaa aaatgttgga ataactaaag
     7861 gtgttgttaa aggagaaaaa acattaaatc aattagcaga gatgtttgga atagaaagtg
     7921 atgaaaagta tgataaatgt ataaaggttg cagacaaagt tatcaacgat ttgtataaat
     7981 caagagatga taaaatggaa ttagtagaaa aaatagctta tgcacctaga gtgaaaaagt
     8041 ggaaagagtt aggtataatg cctggtggtg caaagtcaga agtttttgat gcaatagtaa
     8101 aatcatcaac aaatttaaat agtgacccag tagacatgct agttaattgt ttgaacttag
     8161 gtatatcaac aggattatat ggtcttacac taactaatct tttaaatgat gttatgttgg
     8221 gagaaccagt tataagaatg gctcctgtag gatttaatgt aatagaccca gactacataa
     8281 atataatgat aacaggccat caacattcaa catttgcaaa tttccaagat agattaaaag
     8341 atgaagatgt tataaaatta gctcaatctg ttggtgcaaa aggctttaag ctagtaggat
     8401 gtacatgtgt tggtcaagat ttacaattaa gaggagaaca ttatcaagaa gtatttgctg
     8461 gacatgcagg taacaatttt acaagtgagg ctgttttatc aactggagca atagatatag
     8521 ttctttcaga atttaactgt actataccag ggcttgaacc aatagcagat aaatataaag
     8581 taaaaatggt ttgtttagat gatgttgcta aaaaagcgaa tgcagaatac ataggtttag
     8641 acagaagtaa attagatgaa cttagtaaca ctttaataga aaaagcattg gagtcttata
     8701 aggagagaag aggaagtatt gaaatagata taccaaaaga ccatggattt gaacaatctt
     8761 taactggtgt aagtgaaaag aacctgaaag cgtttttagg agattcatgg aaacctctta
     8821 ttaatttaat tgcagaagga aaaattaaag gtgttgcagc tgttgtagga tgttcaaata
     8881 tgacagcagg aggacatgat gtaaatactg ttgaacttac taaagaattg attaaaaaag
     8941 atataatagt actatctgca ggatgttcta ctggaggatt agaaaatgtt ggtcttatgt
     9001 cacctggagc agaagaatta gcaggagaaa acttaaaaga agtatgtaag actttaggaa
     9061 taccaccagt attaaacttt ggaccatgtc tagctatagg aagactagaa atagtagcaa
     9121 cagaattagc agctgaactt ggaattgacc taccacaatt accattagtg ctttcagctc
     9181 cacaatggtt ggaagaacaa gcattagctg atggagcgtt tggtcttgcc cttggcttac
     9241 ctcttcattt agctttacct ccatttataa caggaggaaa attggtgaca gaagtattaa
     9301 cagaaaaatt aaaagactta acaggaggac atgttatagt gaatccagac cctaaatctt
     9361 cagctaatca attagaagaa ataataatag atagaagaaa aaatttaggg ttaaaggatg
     9421 tggaatgcaa tgcatagaat ttttataaat aaagatttat gtacaggatg taagagctgt
     9481 gtactagcat gtatgctaaa acacaataaa gattatgaca tgtacactct tgatttagaa
     9541 aatatagata atgatagtag agggcatata gaattagata gtagacataa taatccagtt
     9601 ccaattcttt gtagacactg tgatgaacct gaatgtgtat tagcatgtat gagtggtgca
     9661 atgcataaag atagtgaaag tggaatcgtt tcatatgatg aagagaaatg tggttcatgt
     9721 tatatgtgtg taatgtcttg tccatatgga ttattaaagc cagatgatag aagcaagcag
     9781 aacatactta aatgtgattt atgtaaagat gaggagtatc ctagatgtgt agctaattgc
     9841 ccaagtggag caattgaatt acagaaggag gaaaatgatg aattatgtag tgttaggggc
     9901 tagtgcagct ggtataaatg cagtaaaaac attaagagaa ttagataaag attcaaatat
     9961 agtagtaatt tctaaagatg aaaacgtata ttcaagatgt atgttgcatc acgttatatc
    10021 tgaacacaga actttaaaac aaataaattt tgttgatgaa gattttatgg agcaaaataa
    10081 tgtaaaatgg attgcaggta agactgttaa aggtatagac ataaataaaa aggtagttca
    10141 gacagaagac attactgtaa attatgataa acttttaata gctacaggtg catcttctgc
    10201 tattcctcct ataaaaaatt taagagatgg aaattttgta tattctgtga gaaacataga
    10261 tgatatttat aaaataaaag aaaaagcaca aaattctaaa aatgtagtta taataggtgc
    10321 tggtttagtt ggtatagatg cattagtggg attatttaaa tatgagcagt taaatatttc
    10381 tgtagctttt atggaaaagt acattttaga tagacaattg gatgaatata cagcatcagt
    10441 gtatgaaaat aagttcaaag aaaaaggtgt aaaattttac ccaagtgcat caatacaaga
    10501 aattgtattg gataacagta aaaatgttac aggtgttgca ttttcaaatg gtgaagtttt
    10561 agatgcagac atggtaattg tagctacagg ggttaaacca aatgctgact ttttagatgg
    10621 aacaggaata gaatatgata gaggtataat aatagatgat atgtgccaaa caacacaaaa
    10681 agacatatat gcagcaggag atgttgtagg taaaaatgct atttggcctc tggcagtaaa
    10741 gcaaggaatt gtagctgcct ataatatggt tggaaaagat aagaagatag aagatgaatt
    10801 tgcttttaaa aacagtatga atttcatgga tataccaact atttctatag gaatgaatac
    10861 acctgtagat gatagctata aagtattaac cagacatgga attaatgatt ataaaaagtt
    10921 tgtttttaaa gataatgtaa tctatggagc agttatacaa ggagatattt catatgtagg
    10981 tgtattaact tatttaataa aaaataaagt tgaaatatat gatttagaaa ataggatatt
    11041 tgatgtatgt tatgcagact tttttaatat aaaagaaaat ggagaatttt gttatagtgt
    11101 atagtatata cgtgaagtaa ggtttataaa tacagtaaat caataaatag agttattcta
    11161 aaataagaaa aatgacaaat cttatttaga aatagctcta ttttttgcaa ttaaatttaa
    11221 attatattca atttgtaaaa tattattttg aacaattata catatactct ctagttagag
    11281 gtaaatcatt ttttatacct ttagtgaaaa gtatttgatg tatatctata ataccagtgt
    11341 tgaaagcagc agcacaagaa caaaggtata aatcccacat tcttataaac ttatcatcaa
    11401 acatttcttt aatctttgga agattctcag agaaattttt ttgccagtat agtaaggttt
    11461 ttacataatg taatcttaga ctttctacgt ctatagtatg atatttgtaa tcactacaaa
    11521 tatgtatcat ttctcttagg ctaggaataa ctcctcctgg gaaaatatat ttttttatcc
    11581 aagggtctcc ataagattcc tgcaatccac ttatatagtg aagtaaaaac aatccaccat
    11641 cttttaaaac agcatttacg tttttaatga aaagttcata gttttctcta ccaacatgtt
    11701 caagcatccc aacgcttaca actctatcaa acataaggtc tgattttttt aattctctat
    11761 agtccataag tcttacctca agatagtctt gaaggttctc ttctttaatt cttgtgttaa
    11821 attccttaaa ttgctcttcg ctaagtgtaa ttccaacgcc gtgaatttta tatttttttg
    11881 cagcttctat tagtaaaaat ccccaaccac aaccaatatc gagaaggctc ataccttcct
    11941 ctaaatttag tttagttaat atgtggtgaa ctttattgac ttgagcatca taaagagtat
    12001 cattatcatt tctaaagtat gcacaagaat aactaagtgt cttatctaac cataagctat
    12061 aaaaatcgtt gccaatgtca tagtgggcat gtatttcttt cttttgatgt ctcattgaat
    12121 ttgatgagtg caatagatgt tttacagctg aattatcaat attgaatttg tctatttgtt
    12181 ttaaaagtag atttaaagtt aagaataaat ctccctcaac ttcaatgtct ctatttatgt
    12241 aggcttctcc aagtgctaaa gatgtacttt ttaatagttc agacttgcta atatcattat
    12301 ttactataac tttgaactga ggggagccct ctccaattag aaattcttcc tcatctttaa
    12361 gcctaactag aaatggtgtt tcaccaaatc tctctaaata gtcttttaaa aaattttttg
    12421 taatattcat attaccagct ccttctaatg taaatccagt aagtttccct gcttaaaata
    12481 tattatagtc ctttttttaa aaaagtaaaa tttttt
//
LOCUS       DAGSWI010000123        12506 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.123, whole genome shotgun sequence.
ACCESSION   DAGSWI010000123 DAGSWI010000000
VERSION     DAGSWI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12506
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.123"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..322
                     /locus_tag="MJF80_003035"
     CDS             <1..322
                     /locus_tag="MJF80_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBZ0281675.1"
                     /translation="CIEDLQVKNMIRNRKLSRLISDVSWSEFIRQLEYKANWHGRQIV
                     KVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLIT
                     IQNK"
     gene            832..2238
                     /locus_tag="MJF80_003036"
     CDS             832..2238
                     /locus_tag="MJF80_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBZ0281676.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            2266..3114
                     /locus_tag="MJF80_003037"
     CDS             2266..3114
                     /locus_tag="MJF80_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBZ0281677.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLSEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3775..4467
                     /locus_tag="MJF80_003038"
     CDS             3775..4467
                     /locus_tag="MJF80_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281678.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            4464..5399
                     /locus_tag="MJF80_003039"
     CDS             4464..5399
                     /locus_tag="MJF80_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0281679.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYMDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            5547..6470
                     /locus_tag="MJF80_003040"
     CDS             5547..6470
                     /locus_tag="MJF80_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281680.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            6454..7224
                     /locus_tag="MJF80_003041"
     CDS             6454..7224
                     /locus_tag="MJF80_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281681.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            7234..8016
                     /locus_tag="MJF80_003042"
     CDS             7234..8016
                     /locus_tag="MJF80_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281682.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            8393..8929
                     /locus_tag="MJF80_003043"
     CDS             8393..8929
                     /locus_tag="MJF80_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBZ0281683.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8961..9350
                     /locus_tag="MJF80_003044"
     CDS             8961..9350
                     /locus_tag="MJF80_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBZ0281684.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            9387..9773
                     /locus_tag="MJF80_003045"
     CDS             9387..9773
                     /locus_tag="MJF80_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBZ0281685.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9900..11342
                     /locus_tag="MJF80_003046"
     CDS             9900..11342
                     /locus_tag="MJF80_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBZ0281686.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            11639..12454
                     /locus_tag="MJF80_003047"
     CDS             11639..12454
                     /locus_tag="MJF80_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBZ0281687.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         4523 a         1546 c         2385 g         4052 t
ORIGIN      
        1 ttgcatagaa gatttacaag taaagaatat gattagaaat cgtaaactat ctcgcttaat
       61 ttctgatgta tcatggtcag agtttattcg tcaattagaa tacaaggcta attggcatgg
      121 tagacaaatt gtaaaggtag gtaaattctt tgcaagttca caaatatgca ataaatgtgg
      181 gtataaaaac gaggaagtga aggacttaaa tataagagaa tggatttgtc ctagttgtaa
      241 tgaaactcac gatagagata taaatgcaag tataaatata ttaaaagaag gattaagact
      301 aataacaatt caaaataaat aacttatgta agaaccgtag gaactacggg gatagcctgt
      361 ggagaattag taagacatat tttagtatgc aaaattctat gaagcaggaa ccctgtgact
      421 ttagtcatgg gaggttcagt ttgggcaagt agtttcttgt aaatctagtt atgtttcaac
      481 caatgataat ataatttcat tagtattagc tggtaattaa gttaatttga ctagagataa
      541 gtaaattgat ttaaaaggaa ataagtaaat caacaattaa atacatctaa aagtaagttt
      601 attgcaatga attgttaaag aaaataatta atatggagta aagctattgc aatgaagtaa
      661 caaagaaaat aattaatata gagtaagttt attgtaatga attgttaaag aaaataatta
      721 atatagggta aatctatctc aatgaaataa caaagaaaag agttaatata gagtgaactt
      781 attgcaatga atcaacaaag aaaataatca atataaaaag gagaaatatt tatgaataca
      841 ggatttccaa aagacttttt atggggagca tcatcttctg catttcaagt ggaaggagct
      901 tgggataaag acaataaagg aaaaaccgta gccgactata attcttttaa aaagtctcat
      961 ttacaggcag atactaaagt cgcatcagac ttttaccata attatgaaga agatattgaa
     1021 ttaatgaaag aattagggat gaaaacatat cgtttttcaa tttcatgggc aagaataata
     1081 ccagatggtg aaggagagat aaaccagaaa ggtttagaat tttataataa gataatagac
     1141 aaactgatag agtgtgatat tgagccattc gtaactttat accattttga ccttccattt
     1201 aaattagtag agaaatataa tggttgggaa agtagagaaa ctgtctatgc ttttgaaaga
     1261 tttgctaaaa tatgctttaa gcattttgga gatagggtaa agtattggca acctcataat
     1321 gaacagaatt taattgttag agtggaggag agaattaata tatatgatga aacagattca
     1381 tggaagatag ataaaatgcg tgctcagatg gattataatc tttgtttagc acatgcatta
     1441 gcagtcaatg catgtcatga gatgataaaa gaaagtaaaa ttggagcagc agtatcatca
     1501 tctgtaacat atcctcttac ttctaagcca gaagatgtat atgcagctag aatgaatgat
     1561 aattttaaag tgtactatat gcttgatatg catcattatg gagaatatcc agggtattat
     1621 atgaagtatc ttgaaaaaag aaatatagtt cctcacatgg aagatgggga taaagagata
     1681 cttaaaaaag ctaaaatgga ttttatagct gtaaattatt ataggacaaa ttgtgcagaa
     1741 gctttacctg aagattcaca acatccattt ggattaagag aaggaactgt tgattttagt
     1801 atgtatggac tatttaaaat gtcaatgaat cctaacttag aggcttctga gtatggagca
     1861 gcaatagacc catcaggtct tagagttgcc ttaaatgaat attggcaaag atatcactta
     1921 cctgttataa taacagaaaa tggtctagga gctaaagata tattagaaga tggaaaaatt
     1981 catgatgact atcgtataga ctatttaaga agtcatataa atgcatgtaa actagctatt
     2041 gaagatggtg tagagatgat tggatattgt ccatggtcat ttacagacct tttaagttct
     2101 tctcaaggtt ttaataagcg ttatggactt gtttatataa atagaactga ccatgaagtt
     2161 ttagatttaa aaagaatcaa gaaagatagt ttttactggt ataaagaagt tatagagaac
     2221 aatggaatag taaaataaac ataaatatat attgagggtt ttagtatgta cattacaaag
     2281 gtattaaata attcgttgct acttgcaaag gatgataatg atgaagaaat aatattgatg
     2341 gggaaaggca ttggccataa ttataaggcc gggtctgagt taaataaaaa agatatagaa
     2401 aagatttttg ttcttcatga tgaaaatata aaaaagagta ttatacaatt ggcaacagaa
     2461 atagatgcag aatattttgg tatagctcaa atgataattg catatggaat agaaaaatat
     2521 aacttaaaac ttatgaacca tatatatttg gccttaactg accacattgc ctttgcagtg
     2581 agacgtttta aagaaggtat aatgattgaa aatcactatt tgttcgaaat caaggaattt
     2641 aatccaaaag agtatgatat aggaaaatat gctattactg tttttaagga ggttcttggt
     2701 ttagacctat cagaagaaga gattggtaat atagcaggcc attttataaa tgcacaacaa
     2761 gataatcctt atagtgatag aaataagaga agtgctaaaa ttgtaaatgc tattttgcaa
     2821 atagttcatt atcacttttc tattgtatat aataaagagt cattttatta taggagattt
     2881 gtaatgcatc taaaggcctt ttcacaaaga tttctggcta atgaaccatc aagagaaaaa
     2941 atagatttta tatatgagca agttcaaaaa aattgtaagg aagagtatga atgtgtgaaa
     3001 aaggtaggag cttatatgta taaggaatac tccagagaat taccaagaca agaggagtta
     3061 tatttgatga ttcatattca taagatttta ggagaattat gcgaaaatag ttagaatatc
     3121 ttaatatgct agtaatatta taaaaaatta aaaattaact acactttaaa tatgaataat
     3181 tgcattactt aagggggttg tcttaaaatt gattttttta attaaatttg aagctcaact
     3241 ccttttttaa acctttatat cattacaact ttaatgttta tacgcctgta ctttagatgt
     3301 atatctactt tttacatttc tcaacatgca aacccaccat tttttgcagt atgttaattg
     3361 aaataaacca ttgagttaac cctaaattta ttctggactt ataggaatag ttccaaagat
     3421 actttaaaat attttctgga ataattgctt atttcacata tgttttattg tgatgttttg
     3481 atttaataaa ttcttcaact ctgtatatag taaatgtatt atcatgtttg ttttattcaa
     3541 gcacacttat aatatgcttg attttttcac aatgatgaaa tatactaatg ggtgttatag
     3601 gtataagaat agcattacac ttgaagaagt aagtgataaa tgagatataa atcctcatat
     3661 gataaactac tatttcattg aggagcatat tctaagtgat gtcaagatac tacaatatga
     3721 ctaaattcct agtgataaaa aagtctacga atgagtgtag aaaggggaac taagatgaat
     3781 aaagttttaa ttatagatga tgataaagat ctttgtattt tgttaaaaaa gagtatctca
     3841 attgaacaga ttgaagcaaa ctattgccat tctgggagag atggactggt tgaactttca
     3901 aaacaagatt atcagttagt agttctagac gtgatgatgc caggtatgga tggttttgaa
     3961 actatggaac aaatacgaaa atatagcagt attcccatct taatgctgac atcaaaaagc
     4021 gataattaca gcaaagtgca tggattgcga gctggtgcag atgactatct aaccaaacca
     4081 tttgaaatta cagaatttac tgctcgtgta ctgtctttaa tccgacgtta cacccgtttt
     4141 aatccaaaaa atgaggagcc acagcaactt tctttcaaag gtttgttgat tgaccttgac
     4201 cagcatacag ttactaccaa tggcgaacct atgttgcttc cacccaagga atttggtgta
     4261 cttctttact gtgcaaggca tcagggtaag attttgacaa aacagcagat ttatgaagcc
     4321 gtgtggggtg agccatatgt atatgatgac agcaatatca tggctattat cagtcgccta
     4381 agaaaaaaaa ttgaactaga cccatcatct cccatctatt tgcaaacaat aaaaggaatt
     4441 gggtatcaat tcaacacgga ggtatgaaaa tggacgaaca aacgataatt cttttcttac
     4501 tcttgctgtc gattggtcta ctttttcttt ccatatttgc tttttccaag cttgggcata
     4561 tttacaagcg cctaaaagat atagaggaga tactggctga tgtacaaatt ggagacgaaa
     4621 atcgtaaaat actaattaag ccatgtgatg ttatggctcc attggtttat cagcttaatg
     4681 agattgtcta cgattatgaa aataagttac tttctctgaa aaagtcggat aaagctagca
     4741 aacaacttat gacaagctta tctcatgatg ttcgtacacc attaacaact ctgattggtt
     4801 atatggatgc tgtacacagt ggtatagtta tcggacaaga gcgggaggaa taccttgaaa
     4861 tcgctaggcg tagagcctac gacctcaagg attacattga tgttcttttt gactggtttc
     4921 ggttgaactc tgatgaattt acgctgtcaa tagaatctgt ggaaattgca gagttgtcac
     4981 gcaatatttt gaaagactgg attcctatat tccatgaaaa gaaactgaat tttgaaattg
     5041 atattccaga aaatcgacta atggtaaacc tcgacccaga tggatattcc cgtgtggtca
     5101 ataatctggt tcaaaatgtt cttgctcaca gtaaggctcg gcaaatcaag attaccatga
     5161 gtgaggattc cagaatggta ttactccgtg ttgaggatga tggtgttggg attgccagag
     5221 aaaatttacc acatattttt gagcgactgt ataagtgtga taaaggacgt tcagaaaaag
     5281 gaagtggtct tggtctatct attgtcagtc aaatggtgga gcgaatgggt ggtcaggttt
     5341 ccgtagaaag tgagattgga aagtataccg tatttacagt gagcttacca ttaatataaa
     5401 attgtttttc ccccatgtga catgtataca cagggggatt ttttgtaagt tataaagttg
     5461 caaggttaat acaaggttgt tgcaaggtta atgtaagatt gaggtggtat gataacttta
     5521 ataaacaaag gaggctttta ttatttatga ataatttaat tattgaaaca aaaaatctca
     5581 ctaagcaata tggtgctcaa aagagcgttt ctaatttgaa tattcacgtt cagaaaggtc
     5641 gcatttacgg attgctagga agaaacggtg cgggtaagac gacaactatg aaaatgttat
     5701 tgggtctaac aaaaccaact actggagaaa ttaaaatatt tggtcaggac attcacaata
     5761 acgagaagaa gcttcttcct cgaattggat gtttgattga atcaccaggg ttttatccta
     5821 atctgacagg aacagaaaat ctcaaaatct ttgcaaggct tagaggaata ccaaaaaaaa
     5881 atgcagtaga atcagcactg gatgtagtcg gtttgccaaa caatgacaaa aagttatttt
     5941 ctcaatactc tcttggtatg aaacagaggt tggcgattgc tcttgccatt atgcatgacc
     6001 cacaactgtt gattttggac gagcctatca atggacttga tcctattggt attgcagaag
     6061 taagaacgtt tattcgtgat ctctgcacag aacgaggtaa aactattttg atttccagtc
     6121 acattctttc tgaaattgcc ttgttggcag atgacattgg cattattgac catggtattt
     6181 tgttggagga agaaagtctt gcccaattag aagaaaaaaa cagtaaatac atacattttg
     6241 ctgtatctga tgcagcacag gcagcaaggc ttctggaaac ccaacttaac ctgaaaaatt
     6301 tcaaagtagc agataattac aatattatgc tatatgatac agatgtttct gttgcagaaa
     6361 taaatcgtgc tttcattcta aacaacattg aggtatcaga atctcattta tgcgaagata
     6421 ctcttgaaga ttacttcaag aaagtaacag ggggtgaagg aattgcttga tataatttca
     6481 tgtgaatttt caaaactcaa gcggttaaaa tttatactga tttctatttt aggtgcttgc
     6541 ttatttccaa ttcccgcaac aattttgatt gcaaaggaca acttgccctt tgagcaattg
     6601 ttcaaattag ttgtcaattt tggatatttt ttgctattac caattgtatt aagtattgtt
     6661 gctagccaac tattttttat agaaagagat aatgatgtat taaaaaatct tgcaacagtg
     6721 ccagtgcaaa aaggaaaact ggctctggca aaattggctg tgttgctgtt tatatcattg
     6781 ttctactctg ttgcagggtt aggagctact atcattggag gtttcattgt tggaatagta
     6841 gagggggttg ctgtaaaatt aggaatgagc attgcttttg gaattatgtt ttttgttact
     6901 gtacttcctg ttgtggtact cattgtatat ttcaatcgca gctatatttt ttctattata
     6961 ctttcttttg tatatgctat tttcaatttt agtatatcct taaatattat caattttgaa
     7021 ccaaataacc caatattaag tgtattacct gctcctgtta taatgcgttg gtggatggca
     7081 ttttggggag atcccactgg cgaatacacg gcacttagac agccatattt gctctcaact
     7141 cctgcttgtg taggtatttt atgtttgata accattatag cagtcctgct gatttgtatg
     7201 atatacaaaa agcaggagaa ttaggaggta tctatgcttg atataattac tacagaattt
     7261 caaaaaataa aaagatataa tattctatgg attggtgttg ttgctgttct gttttcagca
     7321 ctattggcag tttttcaggt tgtaagttca cacggcagtg accctctaac gtatgagagc
     7381 tttgcaaacg gagttatctg gaataatttc agtcttagtt ttccatttgg gattacactt
     7441 attggaggat atcttattaa ccgtgaatat acagaccaaa ccttaaaaaa catcctaact
     7501 gttcctattt cattacgcaa attgctagtg ggaaaattaa ttgcggttgg aggaattact
     7561 gtattgttaa gtcttttcag cttcttatgc acatttattt tggggttgtt gttttgcaat
     7621 atcaatatat ctgttacact cgttgtaaaa tccttagttc agattttgtc tgttaatctg
     7681 tattgttatg ttgctgtttt gccgattatt gcttattttg gtcgtaaaca aaatgcattt
     7741 ctgacgggtg tagggattgc atttgtatac ggtttttgtg gtgtgtttgt tgctggaaga
     7801 aatctgacag acttttatcc aatcacagca ggtcttggtc tagttggata caacaatggt
     7861 gctggtactg tttatcagcc acttatcggt gctatgactt tgattgtaat tctaatcttg
     7921 acaactattc tacttgtatt tacacctaat tatgataaag ttatgaccat atctaagaaa
     7981 aaagggataa agcataaaaa gtattctcat aaataacata aatattgttt ctattattta
     8041 agttgctaga cagtgaaaag aaaaaatctg tcgtacagtg tctatatgct catgtattca
     8101 agaagctaca gttttgattt ttcataactg tagcttcttt ttgtatttat taaatcacga
     8161 ctatcataat acttgcaata ggtatagcga caagagagag tactgtcata tgtgttttat
     8221 atattacatt aagtatattc taaaatatat gttttaaata atatatttga aaataggaat
     8281 atttaaataa aaaaaataat attatatata gttgacaaaa attggcaaat gagatactat
     8341 ataaatatag aaatagtaat cattactatt attaaaaagg gaggaattaa ttatgaactt
     8401 aaaaggaact aaaacagaaa agaatttaat ggcaggattt gcaggagagt cagaagcaag
     8461 aaataaatat acatactacg cttcaaaagc aagaaaagaa ggttataatc aaatagctgc
     8521 tatatttgaa gaaactgcta acaatgaaaa ggaacatgct aaattatggt ttaaactttt
     8581 acatgatggt atgccttcaa cagaggaaaa tttaaaggat gcagcagcag gtgaaaacta
     8641 tgaatggact gatatgtatg caaaatttgc taaagaagct agagaagaag gatttgacaa
     8701 gatagcttat ttatttgaag ctgttggaaa aatagagaaa gagcatgaag aaagatactt
     8761 aaagctttta gaaaacttaa atgaaggaaa aatatttaaa agagatgaag aagttgtttg
     8821 gcaatgtcaa aactgtggtc atgtgtatgt aggtactgaa gcaccagaaa aatgcccagt
     8881 atgtgaccat ccaaaagcgt atttcaatat aaaagctgaa aattattaaa aattatcatt
     8941 attaaaaagg aatgttaact atgaaatttt ctaaacaacg agaactgatt ttaaatgaaa
     9001 tattaaataa tccggttcat cctactgcgg attacctata tgaaaactta aaaaaagata
     9061 atccaaattt aagcttagga actgtgtata gaaatttagc tcagctaacg gaacatggct
     9121 ttataagaaa ggttagtatt ccagggtatc cagatagatt tgatggtcga atagataatc
     9181 actatcatat catatgtgaa gtatgtggag aggtatacga tttagaatcc gaagttctta
     9241 ataatttaca agaattaata tctgaggaga cagacataaa aataacatct tataacataa
     9301 gttttaaagg gatttgtaat aattgtaaaa ggtgtagcca agtaggttag ttttcaaatt
     9361 ctaaaaataa ttaatgaggt gagtttatgt gtagtgaaca aaaatttttt atatgtgaga
     9421 cttgtggaaa ccttgtagga atgatacaaa gtggtggtgt acctattttc tgttgtggaa
     9481 aacctatgaa ggaacttgtt cctaatacaa cagatgcagc agttgaaaaa catgtacctg
     9541 taattgaagt ggatggaaat aatgttactg ttaaagtaag tagcacaact catccaatga
     9601 ctaaagaaca tcatatagca tgggtgtatc ttatgactga acaaggtggt caacgcaaat
     9661 gtctagcagt agatggagaa cctgtagtta aatttgcttt gaatgatgat gataaagtga
     9721 tttctgccta tgcttactgt aatttacatg gattatggaa ggctgaactg taatatttaa
     9781 ataaattgag tcaaaaagga ggctataaat gtattttcat acaaatagcc tcctataaat
     9841 ttatataaat tcaagtttta attttgttta atgtcaatat aatataagga gaaaataata
     9901 tgtcaattta taaatgtagt gtttgtggat atatttatga tgaatctaag aatgataaaa
     9961 catgggatga attaagtgaa gattgggaat gccctgtatg tacaaaaggt cgtagttatt
    10021 ttggaaagat aagtactgtt tactatgaag aagatgaaaa gatagcagaa gacatagttg
    10081 aagatgaatc taaattaaat actgaaaaag agggagattt aaattactta agtacatatt
    10141 taagaagaga tgatgaagtt gagaaacata tggatataat tcacgaaatg gctgttacag
    10201 gaaaatctat aatagaacct atgagaacta aactaccagt aatttcttgg gatgatattt
    10261 taattatggg tgctcaatta aatccattac ctttaaatga gcatgatgaa gtaaatacaa
    10321 caacaataat aggaaaaaaa gcaaagaaac ctatgattat agaaaatcca gtttatattt
    10381 cccacatgtc atttggagct ttgtctaaag aactaaaaat agctttagca aagggtgcag
    10441 cccaaaataa gacagctatg tgtagtggtg aaggaggtat attaccagaa gagaaggaag
    10501 cttcatacaa gtatattttt gagtatgtac caaataaata tagtgtaact gaagaaaatc
    10561 ttaaaaattc tgatgctata gagataaaaa ttggtcaagg aacaaaacct ggtatgggag
    10621 gtcatcttcc tggcgaaaaa gtaactgaag aaatagcaaa agtaagaaat aagcctgtag
    10681 gtcaagatgt aataagtcca tcttgttttg aggaaataca atccaaagaa gatttaaaga
    10741 aacttgtaga tgaacttaga gaagtttcag aagggcgtcc aataggtgtt aaaatttcag
    10801 caggtcatat tgaaaaagat atggaattta tagcttatgc taaacctgat tttgtaacta
    10861 ttgatggtcg tggtggagct actggagcaa gtccaaagtt gcttaaagat gcaacttcaa
    10921 ttccaacaat atttgctctt tatcgtgcta gaaaatacat agatacacat ggattagata
    10981 tagatttggt tataacaggt gggcttcgta tatctactga ttttgctaag gcaattgcta
    11041 tgggtgctga tgcagttgct attgcaagtt ctgctcttat ggcagcggcg tgtcaacaat
    11101 atcgtatatg tggttctggt aagtgtcctg taggagtagc tactcaggat gaggaattga
    11161 gaaaaagatt acatatagaa aacagtgcta atagagtggc aaacttttta aatgtaagtt
    11221 tagaagaact taagactttt gctagaattt ctggtcataa agatatacat gatttaagtg
    11281 ttgatgattt atatactgtt aactctgaaa tatcaaatta tacaaacata cagcatgtat
    11341 agattgaagt ttgaaactat tagattttag atagctataa aagtaaatga taaatatatt
    11401 ttataaaaga gagtgtctta aaatgaactt tttagttcat aagtcactct tttttttatg
    11461 aagccaaata tatttatcat tttagcaatg aaaaagaatc ttatctcttt attttgagat
    11521 agactccttc tatttatgaa aattaaagat attaaattgt aaaatattct gtgatgtcgt
    11581 actattaaac taaagattct gtactttaaa gtaaataaaa atttaaggag tgattattat
    11641 ggaaagattg aagatacaag ttttagttga caataatact tatattgata gatactttgt
    11701 tggagaacca gcagtaagct attatattga gattgatgga aataggattt tgtttgactc
    11761 agggtattca gatgtattca tatcaaacgc tgaaaaatta aatatagatt taggaaattt
    11821 aactcatgtt gttttttctc atgggcataa tgaccataca agaggtatcc agtttctaga
    11881 aaataggtat gatttatcta cagttgaact tatttcacat cctaactgtt ttatacctag
    11941 aaaacatgga gagaaaagca taggagctcc attttctgct gaggaaatta aaaatatttt
    12001 tatgtataat cctaaggata agccatttaa tctaagtaag aattgtgtgt ttttaggtga
    12061 aataccttct ataaatgatt ttgaaaaaag agctaaaatt ggtaaatgta aaataggaga
    12121 tttgtgggaa gatgattatg ttttagatga ttctgctata gtttgtaaaa ctgataaagg
    12181 tatttttatt gttacaggat gttctcatag tggaatttgt aatatagtag agtatgctaa
    12241 aaaggtatgt ggagatgata gagttattgg tatactaggt ggttttcatc tttttgaatt
    12301 gaataatagg ttagatagta caatacaata tttggataaa gaagaaatga gaatgttgta
    12361 cccttgtcac tgtgtttctt ttaaggtaaa agcgaagatg aatgaaatat tgaatataaa
    12421 tgaagttgga gtagggctga ccataagtat atagtatttg aaagtatata agatttaagt
    12481 tgaaggttaa aaagatattt tctata
//
LOCUS       DAGSWI010000124        12418 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.124, whole genome shotgun sequence.
ACCESSION   DAGSWI010000124 DAGSWI010000000
VERSION     DAGSWI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.124"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            404..766
                     /locus_tag="MJF80_003048"
     CDS             404..766
                     /locus_tag="MJF80_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBZ0281688.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            831..1448
                     /locus_tag="MJF80_003049"
     CDS             831..1448
                     /locus_tag="MJF80_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBZ0281689.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSISDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            1445..1930
                     /locus_tag="MJF80_003050"
     CDS             1445..1930
                     /locus_tag="MJF80_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBZ0281690.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            1973..2491
                     /locus_tag="MJF80_003051"
     CDS             1973..2491
                     /locus_tag="MJF80_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBZ0281691.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            2627..3586
                     /locus_tag="MJF80_003052"
     CDS             2627..3586
                     /locus_tag="MJF80_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBZ0281692.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            3612..5315
                     /locus_tag="MJF80_003053"
     CDS             3612..5315
                     /locus_tag="MJF80_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBZ0281693.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            5576..7678
                     /locus_tag="MJF80_003054"
     CDS             5576..7678
                     /locus_tag="MJF80_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBZ0281694.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            7691..8131
                     /locus_tag="MJF80_003055"
     CDS             7691..8131
                     /locus_tag="MJF80_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBZ0281695.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            8167..8970
                     /locus_tag="MJF80_003056"
     CDS             8167..8970
                     /locus_tag="MJF80_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBZ0281696.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDRTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            9010..9606
                     /locus_tag="MJF80_003057"
     CDS             9010..9606
                     /locus_tag="MJF80_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBZ0281697.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            9712..10575
                     /locus_tag="MJF80_003058"
     CDS             9712..10575
                     /locus_tag="MJF80_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBZ0281698.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            10642..11631
                     /locus_tag="MJF80_003059"
     CDS             10642..11631
                     /locus_tag="MJF80_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBZ0281699.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            11723..12334
                     /locus_tag="MJF80_003060"
     CDS             11723..12334
                     /locus_tag="MJF80_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBZ0281700.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         5020 a         1219 c         2335 g         3844 t
ORIGIN      
        1 aaatataatt aagtaaataa acaactatct attagatatt gagaaagtca aatcctatat
       61 gaatctacag aaatgatttt aatgaatctg aaaatttttt ttatacaaac ataaaaaagg
      121 tataaagttg ttttattata atttaattac ttttgataac tttttatgtt ttatacaatt
      181 atatttttaa taactcgtca aaacgccaaa catatattac aatagatata taagtttttt
      241 tgttgacaaa ttatgttaag agaattaagt ataatatggc ataaataatt acttttattt
      301 atcaatgaaa tgtatttatt gtgtagcgaa aaatagtcag atagtataga caataactga
      361 agataaaagc gtttattgtt gtacatatgg aggagttagt tttatggaaa ataaaatttt
      421 gatagtagat gatgctattt ttatgagaat gataattaaa gaagctctga gtaaaagtgg
      481 ctataataat ttaatagaag caaatgatgg agaagaagcg tgtaatatat ttaaatatga
      541 aaagcctgat ttgatgttgc ttgatattac aatgccaaaa aaagatggtt tagaggtgct
      601 aagagaaata aaaaagttgg attcaagtgc taaggttgta atgtgctctg caataggaca
      661 agagaatatg attgtagaag ccataaaact tggagcgtta gattttatag tgaagccctt
      721 caaaacagaa acattagtta aagttgtaga taatgcttta aaataaaagt gtaaatatac
      781 actgatacaa tgaaaagcaa ataaatgcaa taataaaggg aggggtttat attataaatt
      841 atttagaact tgatgatgtt catatagatg ctcttagaga aattggtaat ataggctctg
      901 ggaatgcaat tactgcactt gcatccatga taaatagcaa tgtagaagta ttaataccta
      961 tggtcaagat acttgaatat aatgaagcga caaatttgct tggtggtcca gaaaacaaag
     1021 tagtatgtat tttactcgat atgaaaggtg atatcaatgg aatgtttatg tttttgttgg
     1081 atgaatcaat aactcaactt atgctaagtt ctctttttaa taaagaagaa gcttttttag
     1141 atgagattga ggcaattgaa atttcagcaa ttaaggaaat aggaaatatt atggcaagtt
     1201 catatgtaaa tgctattgca tctatgttaa acatgactat aagtgtttca atttcagata
     1261 tatgtattga tatggttgga gctgttctaa atgtacctat gataagattt tcagatgttg
     1321 gtgacaaagt cttatttata gaaaataaat ttaaaatgag tgataattat tttactagtc
     1381 acattttgat gattccagaa atgagctcac ttagagaaat actcgtgagg ttgggattag
     1441 aagtatgaat agagagatag tagtgaacat tgcagatatg aagatagcct atagaccaaa
     1501 tgttttagtt acttatgcac ttggttcttg tgtaggagta tgtttgatag ataaagtagc
     1561 tggaattggt ggaatgttac acgttatgtt gccatatagt aaagatgcta ttaacattga
     1621 aaataagtgt aaatttgctg acacaggaat aaatgagctc attaaatcta tggagaatat
     1681 aggtgcaaat agaatatata taaaagcaaa aattgctgga ggtgctcaga tgtttttagg
     1741 aagtagcaat tcagtaattg caagtatagg acataggaat gttgaggcag taaaaaaaac
     1801 tcttttagga ttaaatattc caatattagc tgaggacaca ggtatgaatt atggtagaac
     1861 aatacgtttt tacactgaga cgggtgaatt acttgtagat tcaataaata aaggtaagaa
     1921 gaaattataa tatatatttc atagaaagtg tattttagga gggaggtatt aaatgagatt
     1981 tccagatgac ccatatttag attatggact aattgatgaa ggaattaagt atctaaagtt
     2041 taaagtagat gagcaagatt ttggggttga acttgaaaag gtaatagaaa ttactggaaa
     2101 tcaagaaatt atatttattc cagaactacc tacatattct aaaggcataa taaatctaag
     2161 aggaaaaatc attccgataa tagatatgcg tttgagactt aaaaaagatg aattaatgac
     2221 tacaaattgc atgtatatag tagtaactga aataaaggac ttggtagttg gatttgtggt
     2281 tgatagagtt gatgatatag taagcttaga aaaaagtaaa atatcacctc caccaagagt
     2341 tactatagaa tattcggatt attttgtaag tggagttgga gaattagaag gtaaaataat
     2401 tactttacta gatttggaga aacttttgac agataaagat gaagttttaa tagatgaatt
     2461 aatcaatggg tttgattgca ctatcatgta acatagggat gtagttggag tgttattata
     2521 aaaagattgt gatgagaaca tttaagaata tagtggtata attattaagt ataaaaattt
     2581 taaatcatta aaatttgaat atattatgtt taagagaggt ttgtatatga aaaataagca
     2641 acagggaaca attttagtcg ttgatgatag tgttctgatg tgtgacttaa tagataaaac
     2701 acttacaaaa gataatttta aggttaaaaa agcatttaat gcaatagagg caaaagaatt
     2761 tattgaagaa tttataccag acattatttt gcttgatata attttaccag ataactctgg
     2821 ttttgagttt tgtaaggaag taaaatctaa tgctcgtaca gcagatatac ctataatttt
     2881 tataacttca aaaaatacag atacagacat agtaaaagga tttggtgtag gagcaattga
     2941 ctatatggtg aaacctttta gtatgacaga attaaaagct cgtgttatag ctcatcttga
     3001 agtaaagaaa tctcaagata aattaaaaaa gataaataat gaactggaat cttctcttga
     3061 aaaattgaat agactcgttg taagagatta tcttacaggt ctttacaata ggcgacatat
     3121 aattaatcag cttatggagc aaagaagaat taataaatat agtgaaacat caatatccct
     3181 tatccttggt gatattgatg actttaaggt tatcaatgat acttatgggc atgaagcagg
     3241 ggatttcgtg ttaacagtaa tatcaagtat aataaaaaag aattgtagac aaattgatac
     3301 tgtatcaaga tggggtggag aagagttttt gataatgctc acaaatactc caatagaagg
     3361 tgctaagata ctttcagaaa gaatccgtaa agagataaag gagtttgatt tcaactataa
     3421 agaacataaa ataaagtgca ctattacatt aggagtagta gaagtaaact caaatatatc
     3481 attagaagaa aacatatcct tagcagataa agcaatgtat gagggtaaaa acttaggaaa
     3541 agattgtagc gtagtgagta caatgaaagg attaaaaaag atataaactt actttatgaa
     3601 ggagtacaga tatgaaaaga gaagtaaagc tttctaaatt agcaattgta ttgctgatta
     3661 ttatagtatt ggctgttcta gcagggattt tttcttctat ttcatcttat aaagcatttg
     3721 aaaaagtaga aaaaagtatt aatgatatga ataatttgag gactcaaagt cagtatataa
     3781 aagatacaac tagagagata agtgatgtat taagaagata tgtatttaca ggagaaaaaa
     3841 agaataaaga tatttatgaa aaagaattat acggaaaaac aagagaatca gctttaaaca
     3901 aattagaaaa aattggtctt acaaagagcg agtcaaataa aatcttagaa gcaaaagaaa
     3961 tatcagattc tttagtacca acagaacaaa gtgcaataaa ggcagtcgaa agtaatgata
     4021 agagtaaagc tcaaagtata atttttggta gtgactatat aaactcccaa gagaaaatgg
     4081 attctttaat aaatgaattt caagaggata tagttacccg ttcaaattcc aaaattgtag
     4141 aagtaaaaaa agaattacaa agtctaatat taagaacaat atttatactt tcccttttaa
     4201 taatattgac aataatagaa tatacagtaa taaaattaaa aatagtgatt cctattaata
     4261 aagtagaaaa acacttttca agaattgcat ctggggactt aagaacaagt atagacgttg
     4321 aagaaagtaa atctgaaata gggctattag tgagttctgt caaaaagatg caaagtatgc
     4381 ttttaaatta catagacctt atagacagta cattaactag tattgcaaaa ggagatttaa
     4441 gagtagagat agaccaagaa tttataggtg attttaaatc aattaaaata tctttagaaa
     4501 gtataataaa ctcttttaat ggtgattttt ctgaaataaa tttatcagca gagcaagttg
     4561 caagtgcttc agaacaagtt gcagcaggtg cacaagctct ttcacagggt gctactgaac
     4621 aagctagttc agtagaagaa ttagcttcca cgattaatga gatatcagat ggaattaaga
     4681 agtgtgcaga tagtgctagt cttgcatgtg aggtatctga ccaatctgca tcacaagttg
     4741 gttttggaag tagatgtatg gatgatatga tgacatcaat gggtgaaata agtgaaaaat
     4801 cgagtgaaat atcaaaaata atcaaaacaa tagatgagat agcattccaa actaatattc
     4861 ttgcattaaa tgcagctgtt gaagcagcaa gagcaggtca atatggtaga ggatttgcag
     4921 ttgtagcaga tgaagttagg tctttggctg gtaaaagtgc aaaagcagct caaaatacag
     4981 ctattctcat agaagaagct attaaaacag tagaaaatgg tgtcagaatt gctaaagaga
     5041 ctgcaaatgc attggacttg gtagtaaatg gtgtagaaaa aactactaat tatattaatg
     5101 gtatttcttc ggctgtacat agtcaagaag tagcaattac acagatttta tctggaatag
     5161 agcaaatttc catagtagtc caaatgaact cagcaacagc agaggaaagt gcagcagcct
     5221 cagaagagat gtctgctcag gctcaaattt taaaagaatt agtagaaaaa tttcatctaa
     5281 aggaaagtga agtaagagaa tcatcatggg tttaaattat gaggtgaata ttgaagtaag
     5341 agaattatca tgtatttaaa ttatgagatg tatatatatt gaaataagag aattatcaag
     5401 gatttaaatt atgagatgta tatatattga agtaagagaa taattgtgta tttaaattat
     5461 gaggtacata tagtgagata agagaatcat caaggattta aattatgaga tgcatatata
     5521 atagaaaatt atttgtaggt cgtaggtgat aaatgtgaat tatattgaac cgagtatgag
     5581 acaaatgtta gatatgttta tatttgagac aagtttatta ctcgaacaat tagatgaaat
     5641 tatgattcaa atagaaaaag agaaagtatt tactcataac aacattaatg aaacttttag
     5701 aataatgcat acgataaaag gttcagcatc tatgatgggg ttagacaata tatctgcttt
     5761 agcacatagt gtagaagact tgttttatgc tatacgtgaa ggtaaaatag aagttttaga
     5821 caatgaccta ttatttgatt tagtctttca atcattagat tttataagaa gagatataga
     5881 tgagttacaa tcaagaggtt gtatatcttc tgattactct aattttattg aaggaattga
     5941 agagtatgtg agtactttgg aattatcaag ttctatgaaa tcaaaggata atgcaaattt
     6001 agaaagtaaa gacgaaaaag aagtaatcga ttcaaatttt gaatacaaag aagactctga
     6061 tgtagtaaaa ataatctttg aacaaggttg catgatggaa aatgtgagag ctttcatgct
     6121 tgtatttgaa cttagagatt actgtgaatt tatagatttt tatccaaaag atgtagaaac
     6181 aaatgcagag tctacagagt atattaaaag ttatgggttt tatatacaat ttgtagctaa
     6241 agaggataag gcaatagttt ataaaactat agaagataat ctctatgtaa aagaatacat
     6301 aaatattagt aaaaaagaaa tagaaatgaa aagtacatca aacaatgatg cagtagaaaa
     6361 taaattaaaa gataataata aagtaagtaa aagttatcaa aataactata cagaagatga
     6421 gatacaagaa gatgttttaa atgaaaaaac tgatatggaa aaagatagtc taagtgaaga
     6481 aaatgaacat ttaccaataa gtaagcagag tattatgagt gtaaatttag aaaaattgga
     6541 cttacttcaa aatatagttg gagaaattgt aattatggaa tcaatggttg taaatagcgt
     6601 aaattctaca ggtgttgata tagataattt caataaggca acaagacaat tacacaagct
     6661 tacagatgaa ttgcaagata tagtaatgtc tatgcgtatg attcaaattg ctggtgtatt
     6721 tcaaaaaatg catagaattg tacgagatat gaataaaaaa ttagataaaa atgtagagtt
     6781 aataactata ggagcagaaa cagaggtgga taagagtgta attgataatt tagcggaacc
     6841 ttttatgcac cttataagaa atgctatgga ccatgcaata gagcctattg aggagcgtat
     6901 agcgaaaggt aaagaagaag tgggaaagat agttttatct gctgaaaatc taggcggaga
     6961 agctgtgata agtatttcag atgatggtag aggcattatg agagataaga ttttggcaaa
     7021 agctaaggaa caaggtcttc ttaaacatca aagtaatgag tatactgatg acgaggtcaa
     7081 ttctttgatt atgactccag gcttttctac aaaagaatct ataactgaat tttctggaag
     7141 aggagtaggc atggacattg ttcgtaaaag tattgaaaaa gttggaggtt caattgaagt
     7201 aaaaagtaaa caggatgagg ggacaacctt tactataaaa ataccattaa ctctttctat
     7261 agtagagggt atgaagttta aagttggaga ttcactattt actttaccaa tatcatccat
     7321 aagaagaaca tttaaactta cagacttaaa tcaaataatt acagaaggaa ataagacaga
     7381 gttaattaca atatatggag catgtattcc cattataagg ttacataaaa tatataatat
     7441 tcaaacaaaa gtaaccaatc tgatgaatgg taatatgata attattgaaa atgagcataa
     7501 agcagtatgc atttttgttg atgaaataat aggtaatcag caagttgttg tgaaaccatt
     7561 tccagtttat tttaaccgat ttaatataaa aaataagggt ttatcgggat gtacaatact
     7621 tggggatgga agtataagtt taattataga tgcagatagc attatagaaa tatattaggg
     7681 gatttaaagt atggctaaag atgaaattat taaacaagta ttaacatttt atgtcaatga
     7741 tgttatttat ggaatagaat tagaaaatgt gattgaaacc ataagatttc aatcaataac
     7801 gtatgtacca tgtcttccaa catatataag tggagttatt aatttgagag gaagaataat
     7861 accagttatt aatatgcata taaagtacaa tcttcctaaa gctgattata atgagagaac
     7921 ttgtataata attacaaagg ttgatgagta tcaagttgga attattgtag ataaagttgt
     7981 tgatgttatt catatagaaa atctaaattt actagagacg actaattcta acaacactaa
     8041 tattaataaa gatataaaag agattgcgaa agttgaagat aattctatat taattttaga
     8101 tattcgaaag tttttgatta atagcatata gttagttctg ataaattagc aggaggaagt
     8161 ttttgcatga taaaactcac agatgaagaa tttatagttc ttgtaaacca tgtaaaaaaa
     8221 gaatatggta ttgatttaag taaaaaaaga gcgttaattg aaggtcgctt atacaataca
     8281 atgatagaga aaaaacttag ttcttttagt caatatatga atctattatt taaagatagg
     8341 acaggaaatg aagctataaa cctaataaat agattgagta ctaaccacac attttttatg
     8401 agagaacctc aacattttga gtttatacag aatagtatac ttcctttttg ggaagaaaat
     8461 aataaaataa gaaacataaa tatatggagt gcaggatgtt ctagtggaga agaagcgtat
     8521 tcaatagcta tgactttgga tgattatttt ggatataata aagagttgtg gaacattaaa
     8581 atcatagcca cagatatttc aactaactca cttgaaaaag ctaaaagagg tatatacata
     8641 gaatcaaatg ttaacaatgt tcctgagtta tggaagaaga agtattttat agacaacaag
     8701 gatggtacat ttaaaatttg tgataagatt agaaaaagtg taatttttaa gccgtttaat
     8761 ttaatgaatg atttctcata tcaacatttt gatttgattt tttgtagaaa tgttatgatt
     8821 tattttgatt taaaaacaag agaagaactt ataaataaat tttataatgc taccaaaaaa
     8881 gatagttttt tgtttgtagg acatgcagaa gtaatcaaca gaagtagtac taaatacaaa
     8941 tatgtaaaac ctgctatata taaacgataa aaaggtattt ttgatatatg attttttgga
     9001 ggcggtaatg tggaacgttt taataatatg gttattgcta taggcgcatc tgtaggtggt
     9061 acagaggcaa tacttgaaat tataaaagac ctaccaaaat caacaccagg gattgttgta
     9121 gttcaacata tgccagctat atttacttat atgtatgcac aacggcttga caagcagtgt
     9181 attatgaatg taagagaagc taagaataat gatagagtag aacaaggaaa tgtgttaatt
     9241 gcacctgggg gttaccagat gaaattgtgt acggataaac aaggatatta tgtaacttgt
     9301 gaaaaaggag aaagaataag tggtcactgt ccatcagtag acgttttgtt tgattcagta
     9361 gcagaagtag caggtaaaaa ttcaatagga ataatactta caggtatggg ttatgatgga
     9421 gcaagtggac ttttaaaaat gaaagaaaat ggttcattta caattggaca ggatgaaaat
     9481 tcttgtatag tatatggaat gccaatggta gcttttgata gaggttctgt tatgttacaa
     9541 ttacctcttg gtgaaatttc aaactgtttg ataagacata ttaatatttt aaaacaaaaa
     9601 gattaatttt ttataaataa aaattaatct tttattaaat tatatggata aaaaacccta
     9661 gaaactccaa atataataag agagatttat atttaggagg aaaatgataa tatgaaattt
     9721 tttaaagtcg atacaaaagc taaagtttta agatatgtat ttgttttaac attgactata
     9781 atggctgttt tggcaattaa acatgactat aaatataata atttaaggaa agatgcttca
     9841 attaatttaa atagtaataa ggaagcagtg actgttatga gtaaccagtc attaccaaag
     9901 ggtaatacaa atagtaaaca aactttatca gtaagtacaa taggaagtaa aacaatagtg
     9961 aaaaatgatg agtatttaga aagtactata aacatgcctg taataacaaa ttcaaataaa
    10021 attgttgaaa ggtctacaaa tgataagata aaaaatgaca tatttgaatt ttataataaa
    10081 tcatataaag aagcaaagca gtatcttaag gataatcctg acgagaaaaa taagtttgtt
    10141 gcaaatgtag actttgaact taaaaagaat actgattctg ctcttagtat aaaagtgaga
    10201 tattatactt atagtggtgg agcacatgga ttttatcaag atatagcata taatgtggat
    10261 atgagaactg gaaaattttt agaattaatg gacttgttta aggataatac taaatataaa
    10321 gaagttatag atgaagaaat taaaagacaa atagctgaac ttgaaaaaaa ggatgaagaa
    10381 aatatcggaa tatataattt taaaggtata aaagagaatc aaaactttta cttgcaagat
    10441 gagaatctag ttgtatattt tgatttgtat gatataactc catatgcagc aggaatacca
    10501 gaattttcaa taaataaagc gttgataagt tctatgctaa aaccagagta tgtagattta
    10561 tttgatttga aatagaagat taaattaaat gttatgctat aatatttagc atagagactt
    10621 attaaaattg ggggtataaa aatgctactt ttaaaaaaag acgacataaa aaaagtattt
    10681 acaatgagag atgcaattga agctgataag gaagctttta gaatttattg tgaaggaaag
    10741 agtgtaaatc ctttgagaac caatatatct gttccaagtc aagatgctag tatgttattt
    10801 atgccaggat acgtagaaga attaggctgt gggggaataa aaattgtatc tgtatttcct
    10861 aaaaatgctc aaaaaggcaa accagtaatt ccagcaacag tactattatt agatggagaa
    10921 actggagaag ttagtgcagt attggatggt acttatgtaa ctcagattcg tactggagca
    10981 gcctctggag cagctataga cgtattggct aaaaaagatt ctaagattgg agctttgata
    11041 ggtctaggag gacaaggtga gccacaactt gaagccatga tagaagctag aaatttggat
    11101 gaggtaagag tttttagtag aaacaaggaa agtagagaaa aatttgcaga agaaatgaac
    11161 ttaaaattaa gtaaatataa tactaaaata gttgcagttg aatcatctga cgaagccata
    11221 gataacgcag atgtgatagt tttagctact ccatcaaaac aaccagtact taatggaaac
    11281 ttagtgaaaa aaggagcttt aataagtgca gtaggttcat atatgccaga tatgcaagag
    11341 ttgtgcccaa actgtttaac tagagcgtct aagatatttt ttgaatcaac agatgcagtt
    11401 ttatctgagt caggagatat attaattcca ttaaaagaag gtaagataag taaagatgat
    11461 tttagtggtg atttaggaaa tgttataaac ggaactattc ctggaagaga agatgatgat
    11521 gagataattg tatttaaaac tgttgggata ggtgtacaag atgtcgtaac agcaaaaaga
    11581 atatacgata aagcaaaaga aaacggtatt ggaatagact ggaagtggta aaatattcaa
    11641 aataattcta tagttagaca aatttatgtt ataatgttga taaataaatt ttttattatt
    11701 ttaattgagg gaggatgttt gtatgaaaat ggtaaaaaag ttaatattat ctacgattat
    11761 ggctctagtt ttagttctaa ctatagggtg ttctaatgca gaagaaaaag taaagtcgaa
    11821 ttttgaagct aatttagctg cattagaaat gtataagtat gatgaaaaag gaatttctga
    11881 tgcattaaca gaagctgaat ataaacagat tcaaacttat aacagcaagg acaaggtttc
    11941 aatgattgct caattgacac ctctttatgg taaagaaaat gctgaaaaga tatacgaaac
    12001 aattgttaaa ttaattcaag gtgttgaaaa gacagtaaca gtggaatctt ctagtaagga
    12061 taagataact ttgaaagttg taagtaaagc tttagatact agtaaagtaa tgagtgaagc
    12121 gaacacttta gtaaaatctt atgtaactga tgaaaagaat tacgataaaa aagaaaaaga
    12181 agttcaagat gatatgacaa aaatgattat agacctttac aataagaaac caactaagga
    12241 agttacaact atgattacat atactaaaaa agatggtaaa tggcaaccac ctagtaatat
    12301 acaaaagtta tgtgaaggaa tatatacaat ttaatttatt ttaaagacac aaaactatct
    12361 tttaagcata taataaaatg tttggaagat agtttatttt attttcaaag tataaatt
//
LOCUS       DAGSWI010000125        12068 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.126, whole genome shotgun sequence.
ACCESSION   DAGSWI010000125 DAGSWI010000000
VERSION     DAGSWI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12068
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.126"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..857
                     /locus_tag="MJF80_003061"
     CDS             <1..857
                     /locus_tag="MJF80_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBZ0281701.1"
                     /translation="GAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLNSFQNLKQ
                     ISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRLFNNLNKT
                     FIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITAVHSYIDE
                     ESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDFSKEIERL
                     TEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDENLYVARVN
                     AIYTFANA"
     gene            1217..2515
                     /locus_tag="MJF80_003062"
     CDS             1217..2515
                     /locus_tag="MJF80_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HBZ0281702.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIVNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLETLEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            2705..4096
                     /locus_tag="MJF80_003063"
     CDS             2705..4096
                     /locus_tag="MJF80_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBZ0281703.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSREIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            4273..4860
                     /locus_tag="MJF80_003064"
     CDS             4273..4860
                     /locus_tag="MJF80_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0281704.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            4893..5684
                     /locus_tag="MJF80_003065"
     CDS             4893..5684
                     /locus_tag="MJF80_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBZ0281705.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5865..8282)
                     /locus_tag="MJF80_003066"
     CDS             complement(5865..8282)
                     /locus_tag="MJF80_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281706.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLEKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(8295..8960)
                     /locus_tag="MJF80_003067"
     CDS             complement(8295..8960)
                     /locus_tag="MJF80_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281707.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(9042..10073)
                     /locus_tag="MJF80_003068"
     CDS             complement(9042..10073)
                     /locus_tag="MJF80_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0281708.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(10063..10749)
                     /locus_tag="MJF80_003069"
     CDS             complement(10063..10749)
                     /locus_tag="MJF80_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281709.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(10907..11971)
                     /locus_tag="MJF80_003070"
     CDS             complement(10907..11971)
                     /locus_tag="MJF80_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBZ0281710.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
BASE COUNT         4374 a         1543 c         1725 g         4426 t
ORIGIN      
        1 atggagctat tatcagattt gatgatacaa aaaataatta taataagata aggaaaatct
       61 ttaaattata tgctttagaa atatctaaaa actttgaaag agattgttta aatagttttc
      121 aaaatctaaa gcaaatatct gatagaggtt taagtttagg agaaaaatat atagaagagt
      181 cttttaagaa aggaattgag actatagtaa gttttggaat tataactata gatatcgata
      241 cctttaaaag tgtttattgt gaaaaatatt tagattttaa gagacttttt aataatttga
      301 ataaaacttt tattatacca aataaaaata aaaaaaatgg ccacgcgagc ttcttagaaa
      361 tagaatcgac aataaagaaa atgagcaatt atttatacaa tgattgtttt aaaattcatt
      421 atgctgttgt agatgccctt atagaaaatc gtataactgc ggtacattct tatattgatg
      481 aagaatctat aaaaaaatct aatgccttat ttaataatta taaagatggt tttattggga
      541 aggctgacga atgcaaagta gtaaaacaaa taataacttt aaatccatat agggaagatg
      601 tatacgaatt tttggtaaaa gaagatggag attttagtaa ggaaattgag agattgactg
      661 aatttttagg ctatgatata agagaatata aagcgtcatt aatggatatt tatataaatg
      721 agttaatgga gagcgatgaa aatgatgttg aaaatgcaaa agaaaagatt gaaaaatatg
      781 ctagatatat tggatgtagt gatgagaatt tatatgtagc aagagttaat gccatatata
      841 cttttgcaaa tgcataattt tattaaacat taaatattag atgttcgatt atataaattt
      901 aaataaaaaa atatccttat taataacaaa tagtttatat taaaaaccat tcttaaaatg
      961 agtggttttt tattttgaat agatatattt taaacctaat agcatttcct atctttaatt
     1021 agccaaaaat caactggatt tcatatagac tacataattt taaattacta aaaaagacaa
     1081 tatattgaca aagatggaat atgttgataa aatgaaatag tcaatatggt gtcttaattt
     1141 aaaaatttat ttatgttgca ttagataaag ctgttttata taaaacataa ataattatta
     1201 taaggagaaa tagattatga aagtaaatgt atacaatgta gaatcatttg tggatgaaag
     1261 acttaagatt atggtttcgg acttaattcc aaatactaaa cttaaaataa gtgtgaaaat
     1321 ggaatttcca tggtgcaagg gagaagaatt ttcatcttat ggagtgtttt gttcaaatga
     1381 aaaaggagaa gtagatttag atttagttga acctatagaa ggtacttata aggctgataa
     1441 ttgtatgggg ttaatttatt cgctggaaaa atctaaaact gaaggtaaaa attttgcaga
     1501 aaatatatct attgataaac caataattgt gaatatgata tttgaaactt ctattgaaag
     1561 aaaagaagtt gaattaaaga gaatatttaa atcagaagat attaccacaa aatacatatc
     1621 tgatgagttt ataggaaaat tattttataa acaaaattct aataataaaa caattttaat
     1681 gctaggtggt tctgatggaa atttagacgc tctagacctt ttagcagctc cacttgcatc
     1741 tagaggattc aatgtactaa cagttgcata ctttgcttta gaagggttac cagataaatt
     1801 agaagaggtt ccattagaat attttgaaaa agtatttgag tggattagtg aaaatgaaat
     1861 taccagtact aaagaaatat ttgtacatgg gacatcaaag ggtggagaat tggccttttt
     1921 acttgcttca agatatgaac aaattaaaaa ggttgtagta tcacaacctc acatttattg
     1981 tttccaagct cttaatggat tgatgagtgg aaatgatact tcatcatggt catataaagg
     2041 agagccattt ccatatatca aagtagacaa tgatatattc tttgaagaac agaaaaaaaa
     2101 tatatcaaaa ggtattccat ttggatttaa tagtacttat aagaaaagtc ttaaaagagc
     2161 agagaataga aaagaggcta gaataaaaat agaaaattcc aaagcagata tacttatgat
     2221 tgctggaaaa gaagataaca tatggaatag ttatgaagct tgtttagaga cattagaaat
     2281 ctttgaagaa aataattatc catataacgt agaattatta acatacaata atatgggtca
     2341 tccgttacca attccatata ttatgcctct aaaggagact ttatgtatga gtatgagtgg
     2401 agggattttt tcttcaggtg gaactgtaga aggaaattca aaaggacagt ttgaatcatg
     2461 gaatagaact attgaatttt ataaaaaacc tttatcaagt gatttaaata actaatatta
     2521 agtgatttaa gtaactaaaa atgaagcggt ctttctatat atgcacaaaa tctatcaatt
     2581 ttatagattt aatttattgt ttatataatt tgcatgttat atactaattt taatgacatt
     2641 gacaatttat aaacaattat aaagttgtaa acttataaga gttattgaaa ggtggagtga
     2701 agttatggaa agactcgcta gaagggaaca acctcatata aaatttggaa tgtttaagat
     2761 aagaattcca tttatacatt acagatttga gaaaccagaa gctattcaag gtataatatc
     2821 aagtatgaca agtttaggca caattggcct tagtacacaa attttgggat taccctatga
     2881 agtggcatgg agtatggcta tcattaactc aatattatac tgtttgcatg tttttatggg
     2941 agacccagtt gtacctggct ggattacagc ttctataact cttacaactg catatttgtt
     3001 aaagttcagc atgggcattg aaagaataca agcattaaca gctttacaaa tagatttagg
     3061 aataatattt attttaatgg gtataactgg tgtagctgga aagttagtta gtaagatacc
     3121 aaattctatt aaaggtggaa tactaatggg tgtcggtata tcgactataa tatcagagtt
     3181 taatcctaaa gggagatttg attcttatcc tatatctata acagtaggaa ttttggttgc
     3241 ttgttttgta atgttttctg aaagatttga tactctaaaa gttaagaata aatttttatt
     3301 tcatttagga gaatatggtg tagtatcagc tattttagtg agtttaatag taggtatatt
     3361 tagtagagaa atagaaattc ctagatttag ttttgacaat ttagtttaca tagtagattt
     3421 taaaaatctg ataaatacag tatctccttt tggaataggt tttccaagtg taatgttatt
     3481 tatacaaggg ataccaatgg cttttatgat atacataatt gcctttggtg attttataac
     3541 aggtgaaaac ttagtacttt ctgaaagtga aaatagaaaa gatgaatata tagatttcaa
     3601 ttcaaatagg tctaatgtta taagtgggat aagaaatatt tttatggcaa tcttttctcc
     3661 atatatacca atgtgcggtc cattagctgc gacacttaca ggaagtgttg ctcaaaggta
     3721 caaggttgga aaggaagcca tgcagtctgt ttttagtggc atgggtacat tagtatgggt
     3781 ttcagcaatt tttatctgtt tttatccaat tgcgcagatt gctacacctc ttataccatt
     3841 agcattgtca gtaacacttt tagttcaagg atatttatgt acaaagctat caatggagct
     3901 atgtaataca ggtgttgaga agggattgat tggacttatg ggaggtgtaa ttgctgctaa
     3961 aggcggaaca tggggacttg ctgtaggttt tatactgtat ttcattttaa ttgattctaa
     4021 aaaaagaaaa gaaaaagata ttaattcaat agaagattgt gtatatgaaa tattagacaa
     4081 aaagaagagt atttaaagga aaatatgagt tgctgttatc tattaataga ttaattgcaa
     4141 ctcatgtttt atttataagt tttaggaatt gcaaaaaaat gcaaagcttt ttcttgacat
     4201 aatttcttga tatgttacta tagttatata acgtatgtgt tttataaaaa tttaatggag
     4261 aggtgaacat atatgccacc taaagtaaag ataacaaaag aaatgatttt gaatacagtc
     4321 ttggatatta caagagaagt gggatttgaa gctgtaaatg cgaggagtat tgcaagtaaa
     4381 ttacagtgtt ccactcgacc aatttttact tgttacgaaa acatgaatga attaaaaaat
     4441 gaatttcttg cttttgcata tgaatattat aatcagtatg ttgttaatta tcgtaattct
     4501 aaaagtgtaa gcccttattt actgcttccg ctttcataca ttgaatttgc tcaagaagaa
     4561 acgtatttat ttaagttgtt atttataaat gatttagatt tgatgatgaa agaggcgaat
     4621 gatttttata aagaaattga caatgaaaaa aaggcaagac ttttttcgga aagcattgga
     4681 atagagttgg aatgtgcaaa agtaatattt ttagatttat ttctttatac tcatggcata
     4741 gctgtcttaa cagcaacaaa aaaattaaca ttagatagat atagtgctga aaaaatgttg
     4801 aaaaatatat taacagcttt tataagacaa gaaaaaccag attggaattt gtctatttga
     4861 tgtttattaa ctaatataga agaggtgaaa ttatggaaac aaaacaatac ttaattgact
     4921 tttataatac ttatgatgaa gatagtcgac ttgcattaaa acatggaatg gtggaatttc
     4981 tcactactat gcattacatt gataagtaca taaaatcagg tgattgtgtg cttgaaattg
     5041 gcgctgcaac tggtcgttat tctcacacac tggcacgtca gggatatgat gttgacgcag
     5101 tggaattagt cgaacataat atagaggttt tccataaaaa tactcaatca aatgaaaata
     5161 taagcattac tcagggtaat gctatggatt tatctgtttt cccagataat aaatatgata
     5221 ttacgttact gttaggacca ctatatcatc tttataacaa agaagataaa caacaagctt
     5281 tacatgaggc aattcgtgta acaaaacctg gtggtgtggt ttttgctgca tatgttatat
     5341 ctgatggctg tctcattgac gaggggttcc atagaggaaa tattaatgta tctgaatata
     5401 ttgaaaaagg tttgattgat ccacagacat ttgctgctaa gtctgaacca aaggatttgt
     5461 ttgagcttgt acgtaaagaa aacattgatg atttgatgtc tgcatttaat gtaactagac
     5521 tgcattatgt cgcatcagat ggtttggctt tgtatatgcg tgaagcagta gactctatgg
     5581 atgatgatgc atttgcatta tatttaaaat atcaccttgc tacttgtgaa cgtgaagatt
     5641 tagtaggggt tacaagtcat gcaattgaca tatttagaaa ataaggaata tgcttgttta
     5701 tgtctctggt gatgttgctc ggagatataa gtaagtgtat ctgtataaaa taaaaacaag
     5761 tatagggata gaaaattatg taaagtcaaa attttctatc cctttagctt ttaaaaaatt
     5821 atgttaaaac ttgtataaaa aatggctatt ttttatatac attcttactg agaacgtaag
     5881 cgttctgtaa gtgatttttt ttctatattt cttacaagta attcagtcac taaaacttgt
     5941 ataccaatta atacgaatga aaaaattaaa atagtaaata tagaaaattt aaaattaata
     6001 aagtctaatc taattacatc ttttataaaa gtgcaaagta gatttgaacc aaaataacct
     6061 agtatacttg aagtacatat agctttgata cttatgttta gaccttctct ataaagcata
     6121 tttcgcagtt gctttcttgt cattccaacg gcttgaagta atgcaaactc tttttttcta
     6181 attaaaacgc tgtttattag ggtattggtc atatttagta taccaaagca agaaattaaa
     6241 ccaacaaaaa tgtatgaagc tataattata tattgaaatg ctctagtaat cattctataa
     6301 tcatcttcat atgaataaag cattaaattt gaatttttct gaattatact taacaacttt
     6361 tcttcaactt ctttagaatg ctttttatct attaaaatag aaaaatctaa atttgtatta
     6421 tatggtgtta gagattctaa agttttgtct gaagcaaaaa acatacctcc tgtattggaa
     6481 ttgtcaatga ttcctataat tttcattttc tttttaataa tatcatttcc actaaagata
     6541 ttaaatgtaa ctgtatctcc aacttttaaa tttaatccat aatattttgt tctatattta
     6601 tttataatta aactatctga atctaaatgt gtatagtcta tttttccact gacaatatat
     6661 tcttcaagtg attttgaaag ttcaggcgta atacctttaa ttagagtaat aaattgtttt
     6721 tcgtctctat ctttgatgta aggttctata atattacttt taattgattt ggattcaatt
     6781 aattttttta ctccatgaat agatttaatt tcattcttaa gagaatctgt tagtggatta
     6841 ttttgttgga tttctgtaat atgctcatat aagttatcac tcttaatgct aattatataa
     6901 tcttcgttat agacaaattg ttgagatagt tgttttttga aatctattga attagcaaga
     6961 ttagcgattg aaataaataa aactccacta atgcttagcg atattaatgc aatcaaattt
     7021 ttctttctat ttgactcgat attctttaat gctagatttt ttatatttat ataactaaaa
     7081 ttttttgctt tttttctaaa tttagtttct gtatattgca tggcttcaat agcagatatt
     7141 ttagaaataa ttctagctgg tttaaataaa gaaattctaa cagttaaaaa cattaccagt
     7201 gataaaatta aaattataaa taaatcagct atcatttgcc atgctaatgg ttgaattatg
     7261 taagcaatta tacaagaagc tataacccct aatggaataa aaataattga caaaatattt
     7321 ccttgtagag aaataatctt ttctaactga aaagaagtca tacctaaaga tttcaactta
     7381 gcatacatct gaaccgaatt aacaactgaa atataaaaaa tactatatat aacaaaaacg
     7441 ctagaaaaaa gaacaactat tccaactact aaaactgtaa taatctgctc accatcaatt
     7501 aaatttgatt caatatagtt atcatttaag tatatattat atttcttaat acctaaagat
     7561 aaaccaattt tctttaactg tgtcttaatt gaagctgaat ctttctctgt attttcttct
     7621 gatagtttaa ctaatacatc tacagtatca ggtcttgact cgtcaaaagt attactttta
     7681 ttttgtaaag gtgtagaagc tatattctta aaataatcat ttgaaacgac tgtatagtat
     7741 tgcttggcat tatcttgata ataattttct agaaatccaa caataataaa atcacattga
     7801 acttctttat tcgtaatatc atcataataa tttaatttaa tgctatctcc aatatcttta
     7861 tctaaaccaa attcctgtaa atatttttct gaaatagcaa tttcattttt atttgtaggt
     7921 agtttgcctt tcaatggttg aaaattcata agtttaattg ctgtatcatc tgcataaaca
     7981 atcgacataa tacaatctgt ctttttatta cttccaatta atttgtaagt tcctacgcca
     8041 ctaaagtttt tatctttata tagtatattt tttgcttttt catctacaga tttatatata
     8101 gcatgatatg gagatgcatt aactaaatct acagaggcat tatatgaaac taaggtcact
     8161 gttaataata aacatgtaga aatcattata atgatacctg aaagatatgt tttaagttta
     8221 ttaattttta aatcatttaa agctattttt cttataaatg tgcgattatt atttctaaac
     8281 atatactaca cctcctagtt aacaattttt ccatcttcaa tacgaataat tctatcagtt
     8341 agttgtgcaa tttcattatt gtgtgtaatc attacaattg tttggttgaa ttttttactt
     8401 gtcactttaa gtaaactcag tacatctgaa ctagttctac tatcaaggtt acctgttggt
     8461 tcatcagcta gtataatagc tggttttgtt acaagtgctc tagctatagc aacacgttgc
     8521 tgttgaccac cagaaagatt acttggcata tcatataatt tttcttcaag agaaagcata
     8581 tttataattt catttataaa atccttatct atggtctctc catccaatcc tattggtaag
     8641 ataatatttt catatacatt aagtaaagga acaagattat aattttgaaa tataaaacct
     8701 atatttctgc gtctaaagat tgtaagctgt tcatcagtca tagtagataa ttccttaccc
     8761 tttataataa cctttccaga tgtaggatta tctaatcctc ccatcatatt taataatgtg
     8821 gatttgccac taccagatgt acctacaata gaaataaatt cgcctttttc aatagataga
     8881 gttacaccat caagagcttt tgtaatgttt gaactttgtc cataatattt ctttaaatta
     8941 attacttgta aaatactcat aataatacct ccttaacatc ttgtaaatac agtataaaac
     9001 caaaatgttg cagaatcatt acaaatgaaa tatttaaaaa tttatttatt aggtaagaaa
     9061 atagaaaaag tcgacccttt tccaatatct gatgtaactt taatatagcc accttgaaga
     9121 gatataattt ttcttgaaag atagagacct attccaatac cttctatgtc atggacatct
     9181 tcttcacgat aaaatctttt aaagatttct gcttggcgac tttctgatat tccttttcca
     9241 ttatcaataa tatctatttt tgtatacata acccaacatt ctgtagctac acgtatctta
     9301 ccattagaac ttgtgtattt tacagcatta tctaatatat taaaaatagc ttcagaagtc
     9361 catttcttgt catgaattat atataaattt gggtcacaat caactgtaac ttcaatattc
     9421 tttttttcag catcaaacaa aattccacta agtgctgtag caattgtttc ataaattgaa
     9481 tttttctttt tagatagtgt gattatacct gtctctagtc tggaggtttt taccatagat
     9541 tgcattaaaa aatctaattt atctaattgg ttttccatat ccattaaaaa atccttttga
     9601 atttctgttt taaatgtttg atttagtaga gtagtattaa tcatttttaa atttgcaata
     9661 ggagttttaa tttgatgaga tatatcagaa actagttctt gtaaatcagc tttctctata
     9721 gcaatactat tgcgattttc ttgaataact tcatacattc taattaatct gtgattgata
     9781 cgtgataaaa tagtttcttc ttctaattca atttcaatat tatcttcact attcatcata
     9841 ctatctaaag tgtgacatat tttagaacaa aatataacta tttttttatg aagtaaaaat
     9901 attattaatg ctataaatcc acaaagtact attgtatata ttaataatag agcaattagt
     9961 tttaaatcat cagttattag ataaattgag atactaaata aaatagctgt aaaaattata
    10021 ccaacagcaa ttattaaata aatagaacta aaagaaaatt ttttaaaatt catttctttc
    10081 acctaaccac atatatccca taccatatac cgttttgata tacttaagat tgtctttttc
    10141 aattttgctt ctaatcctac ttataacaga tgttaaagta tgttcatcta caaagttagc
    10201 atctatatcc cagagttttt ctaaaagtac tttccttgtt aaaatacttt taggattttc
    10261 agtaagcagt tttaaagtac gatattctaa tggtgtaaat ataatttgat ttccatttat
    10321 attggcagat aactcagaaa agttaatttc aagatatcca tccctatagt aatcttgttt
    10381 tgtttttatt gtaagatgat ttaaaaaagc tttaattttt tgtttaaata tattgatatt
    10441 aaatggtttt gttatataat ccaaagctcc aagttcatag cctttaatca tatcacattc
    10501 catatcatta gcagttaaaa aaattattgg tatattgtaa ttttccttta tttcattaca
    10561 taattcaaac ccacttttat ctggtaaatt aatatccaaa attattaaat caaattcaca
    10621 ttcaaaaata gactttagtg cagatttagc tgtaaatttt gaagtaatta tatatccatc
    10681 ttcaattagg ttatatgata gagttttatt taatatagaa tcatcttcta ctactaatat
    10741 tttttccata cagttactcc ttttagttta atattttata atgtcaatta tagactaaaa
    10801 acattgctga attgttataa acgagaaaaa gtatttaaaa atttatatat ttaataaaga
    10861 acctgtccta tgataaattc tagtacagat tctttattaa ataaaattat ttcaaccaag
    10921 atggatttcc aggtataaat attcttttac caaattttga agtatactca tctaaagctt
    10981 tttgaataag ctcattatta cccttaaatg aagtagtatt ctttatagtt tcgagatttt
    11041 ggctaaatgg gcaagcagat atacacatac cacaatctgt acctaaatat ctccatttaa
    11101 tatagcatgt ctcatgttca attacccaat tatattttcc atcatcacca attttaatat
    11161 cattagaaag cgaatgagaa gggcagttaa aagagcattt tttacagacc ttacaaaaat
    11221 cttctagtcc aaaatcaaca tattcatctt ctaaaagagg aatatctgta gtaacaacac
    11281 ctaatctaag tctagagcca taatctttat ttgtaagtat agcatttctt cctatatctc
    11341 ctaaaccagc atcacgagct ataaaaacag gcattactaa ataattagca tccatatgat
    11401 ttctagcatc atatcctaaa tttcttatat agtaacttaa aatcatgccg acgatagatg
    11461 catccacata gcactttgat gtttctataa cttcacatat cattggagct ctatttatca
    11521 tttctttatc catctcacat ccaaatacaa ttgcatactt atgagttaga ttaatttctt
    11581 caccgtaatt ttcttcatgt ctacccctat ttgtataaat atgatagtct tttaatttag
    11641 ttataccaac tacagaagct ccatactgct ttgcaagacc cttaattttt tttgtcattg
    11701 actttgcatc tacatctact ttagtatcag aaacttttcc ttcgcataaa gatttgatat
    11761 cagataaaaa atcgaatgca ctacttgcca ttggagagtt taactcattg taagtcattg
    11821 ttccttcact acataaattt ggtctatttc ttatactatc atcaatttct tttttgtcag
    11881 gatttttctt ataatagtca ttataagcag tactatcttt tttgtaattt gctcttgcaa
    11941 acatagtatt tctctcatca acccttttca attctattcc ccctagtaaa aatatattta
    12001 aaaaatataa ctttattgtt gtataattta attatataag aataaagtta tattttcaat
    12061 ttaagcgt
//
LOCUS       DAGSWI010000126        12053 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.127, whole genome shotgun sequence.
ACCESSION   DAGSWI010000126 DAGSWI010000000
VERSION     DAGSWI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12053
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.127"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            182..1573
                     /locus_tag="MJF80_003071"
     CDS             182..1573
                     /locus_tag="MJF80_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBZ0281711.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1827..3620
                     /locus_tag="MJF80_003072"
     CDS             1827..3620
                     /locus_tag="MJF80_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBZ0281712.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIAPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3701..5083)
                     /locus_tag="MJF80_003073"
     CDS             complement(3701..5083)
                     /locus_tag="MJF80_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBZ0281713.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5341..5688
                     /locus_tag="MJF80_003074"
     CDS             5341..5688
                     /locus_tag="MJF80_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBZ0281714.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5706..6740
                     /locus_tag="MJF80_003075"
     CDS             5706..6740
                     /locus_tag="MJF80_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281715.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6789..7838)
                     /locus_tag="MJF80_003076"
     CDS             complement(6789..7838)
                     /locus_tag="MJF80_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBZ0281716.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8113..9648
                     /locus_tag="MJF80_003077"
     CDS             8113..9648
                     /locus_tag="MJF80_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281717.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9833..10519)
                     /locus_tag="MJF80_003078"
     CDS             complement(9833..10519)
                     /locus_tag="MJF80_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HBZ0281718.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10644..12026
                     /locus_tag="MJF80_003079"
     CDS             10644..12026
                     /locus_tag="MJF80_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBZ0281719.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
BASE COUNT         4591 a         1473 c         2012 g         3977 t
ORIGIN      
        1 tgctgaagat actaaaaaaa taatattttt cataatttta aaaataataa taaaaaaatg
       61 aaaatacagg cgataatcta ttgagtaaac atagaaaaga tgatattata agtatgtgct
      121 aaaatggtta aagcaatatt aatattaaat ttaactataa aacaaaaggg ggcgtttttt
      181 tatgtcaagt acagaaagcg tatctaaaaa aagtggtcat ccgaagagtt tttggcttat
      241 ttgcatgaca attttgtggg aaagatttag ttattatggt ttaacagctt tattggtatt
      301 attttttaca gcaagtgtag ctcaaggtgg tttaggtctt tctactcaag aagcaacatc
      361 catgtttggc ttatttacag gtcttatata tcttacacca ttagtcggtg gctggttagc
      421 agataaagtt ttaggtcaac aaaaatgtat ttctataggt gcacttttaa tagcaatagg
      481 agatttcgta ctatttgctt catctacatc tattgcaatg gtttgggtag gtctatcaat
      541 aattatttta ggaaatggtt tttttaaatc aagttgctca aatatagttg gggatatata
      601 tcctaaaact gatgtgtcaa gaaaagatgc agcatacagt ttattctata tggcagtaaa
      661 tgtaggagca tttatagctc caataatatg tggattgctt tctgataatg tatttgcagt
      721 aagaggagca agtggagata tagtatctta tggatataag atggcatttt taatttgtgg
      781 tataggcatg atgttaggta tgattatatt tacacttctt gcaccaaaat tattaaatga
      841 agtcggaaaa tatcctgtga ctaaaggtaa taaaggtgaa aaaattgagt atggaccact
      901 tactaagttg gagaaaaata ggataatagc aatggttatt ttatttttat ttgtggtctt
      961 attctggaca gcatactatc aaacacaaag ttcttttatg atttatgtaa gggataatgt
     1021 aaatagaact ctatttggat ttgaaatgcc agtagcatgg cttacatcac taaatgctat
     1081 attatgtgtg gctttatgcc cagtattagc taccctatgg gttaaattat ctcataccaa
     1141 aagaggtgat ttctctattc cagttaaaat gggattagga atgataataa tgggtgttgg
     1201 gtttatagtt atggtatttg ctacattagc taatggtcca gatggaatgg taaaggctag
     1261 tatattgttt atagtattga cttatatatt tacaacagta ggtgaattat tcttatctcc
     1321 tataggactt gcaatgttca ataaattagc tccagctaag tatgctacgt tgtctatggg
     1381 agcctggtat ctaacaagtt tttttgcaag tttattatca ggaaagattg ctggttttac
     1441 agccacactt ggttttttac agatatttgg aggaatagca acagttttaa taatatttgg
     1501 tttaatatta ttgggaataa gaaatgtatt aacaagatta atggctatgg atttactaac
     1561 aaaagaaaaa taattcatga cgaattattg taaataaatg aattacttta caataaagga
     1621 agttacattt aaatataaaa ttaaatgtaa tttcctcttt gattatgaaa aaaagcttta
     1681 cacaaataga tattataatt aaaattataa ttgtgataat tagagtgtat aagatttata
     1741 taaattatac aaaaaatatt tatgtaataa actttacata cttatatatt tagttattca
     1801 aactattatt tcgaggaggt cttgttgtga atataaaaga tagattaagt ggattaagaa
     1861 aatttatgga agaaaaaaat atagatgcct atatgattcc atcttctgat aatcaccaaa
     1921 gtgaatatgt aggagattac ttcaaatcaa gagaatttat atcagggttt aatggttcag
     1981 cagggacggt tattgtgact aaagacgaag ctgggttatg gacagatgga agatatttta
     2041 tacaggcaga atctcaatta gaaggaagta caataaaatt attcaaaatg ggacaagaag
     2101 gttgcccaac tacagatgaa tatttatata aaaatattcc tgaaggagga actttaggat
     2161 ttgatggtag agttatatct gctagagaag gagctacatt agctgaaaaa ttatctaaaa
     2221 aaggtataaa gatagaatat cagtatgatt taatagatag tatatggcca gatagacctg
     2281 ctttatcaga tagtaaagca tttttattag atgttaaata ttgtggagaa agttttagct
     2341 caaaattagc tagattaaga gaaaaaatga gtgaaaaagg tacttctact catgtaataa
     2401 ctactcttga tgatatagca tggttattca atataagagg tggagatgtt aaatataatc
     2461 cagttgtact ttcttatgca gtaataactt taaaagaagt ttatctattt gttgatgaaa
     2521 gtaagttaaa tgaagaaata ctaaatgaac tagctaagga aaatgttcaa ataaagccat
     2581 ataatgatgt gtatgaattt gtaaaaaata tagacaaaac tgaaaaagtt ttattagatg
     2641 gaactaagct tagttataca atttacaata atataccttg tgaagttgag aaagttgatg
     2701 agttcaatcc agtaatgttt tttaaagctc aaaaaaatga ggtagaatta gaaaatataa
     2761 gaaattcaca tgtaaaagat ggtgtagctt tcactaaatt tatgtactgg ttaaagaaaa
     2821 atgtaggtaa gatggaaatc actgaaataa gtgctactca aaaattagag gacttaagaa
     2881 gagagcaaga aggtttcttt gagccaagtt tcaatacaat tgctgcatat aaagagcatg
     2941 ctgcaatgat gcactatagt gcaactccag agtcaaatta taaattagag gcagaaggat
     3001 tatttttagt agattcaggt ggacaatatt atgatggaac tactgatata actaggacta
     3061 cagttttagg acctataagt gatgaattaa aactacactt tacttctgta gcaagaggaa
     3121 tgataaatct ttctaaggct aagttcttgc atggttgtag aggatataac ttagacatac
     3181 tatctagaag ttgcatgtgg aatatgggaa ttgattatca atgtggaact ggacatggta
     3241 taggatttgt gctaaatgtt cacgaagctc caaatggatt tagatggaga gtagtacctg
     3301 aaagatttga tagtgcagtt cttgaagaag gaatggtaac tactaatgaa ccaggtatat
     3361 atatagaagg ttctcatggt ataagaacag agaatgaaat tgtagttaga aaagctgaaa
     3421 agaatttcta tggtcaattt atggaatttg aagttgttac gttagctcct attgacttag
     3481 atggtatagc accagaatta atgaataagg atgaaaaaga ttatttaaat tggtatcata
     3541 agttagttta tgataaaata tcacctttct taactgatga agaaagagaa tggttaaaag
     3601 tttacactag agctatatag tttgtactag atacaataaa aatcgaggta ttataatgcc
     3661 tcgatttttt attgtatatt ttattaactt taaaaaattt ttaatttgat aattctacta
     3721 attttttatt cattataagt agtataattc caaatatcaa taaaaatgat ggtatcacta
     3781 tgaagacttg taacatacca agttggtcta taatagcttg cgtatagcca gataatttat
     3841 ttgctgcaaa tttgcctaaa aaccaaacac ccattaggaa tgaaatatac ttttcaggcg
     3901 aaagtttatt taccatacca tacccgactg gagaaaagca aatttctcct atactttgga
     3961 atgttaaaaa tccaactacc caaaatacac ttgctttgtg aattgaatta gggtctgttc
     4021 ctgtttgtat aactgctact atcataaaca aaaatgctat ggctaatagt ataaaaccta
     4081 agctcatttt ttttggcaca cttaaatcgc cttttttagt ttgagataat tttacccata
     4141 ctaaagcaga gataggtcca agaataacac aaaggagacc attgtatgaa tctatccatg
     4201 aagtaggaat ttgaaaattg ccaacagtaa gatttatgtg ttcctttgta tagagtgcaa
     4261 tagacataga ggcttggtta tatgctatcc aaaaaaatac tgtgaaaaaa gctaacacca
     4321 atattactat tattcttttt ttctcaatca aagttaaagg tcttgaagcg agcattttct
     4381 tatattctag tgattttctt atagactctc tcccgttaat agggtcatta ttcaaaggtt
     4441 tataaaactt tctgtttcca aatataaaga agaataaacc tattagaacc gtaattgatg
     4501 aaaataaaaa tacatatcta tatccataca tacttataga taatccgctt ttcatagcaa
     4561 accattcatt agcaactaaa ccgcatatta aagggccaaa taaagtacct agattagtag
     4621 ccatatacgt aatagagaaa gctccatctt ttcttgggtc atcattggta tataagtttc
     4681 caaccatagt atgtaagttt cctttataaa aacctattcc taatccagat aaaaatatca
     4741 tgatgtttat atccatctta ctatttgcta tgtatcctag aaaataacca attgaaacca
     4801 ttagcattcc aagtataata cagtttttgg gacctaaaaa tctatcactg acccatccac
     4861 caataactgg agagaaataa gtccatgcta aaaaagatgc aagcattgat gcggattctg
     4921 ctggtgttaa ccctaaacca cctttggaga cttgttcagc taaaaacaat aataatacca
     4981 atttcatacc atagtatgca agtcctacaa aggtaaatat tgaacagcag aaatataaat
     5041 ctctaggttg tttaagtttt tcttgcgttt ttaaaactga catatgtcga gaacctccag
     5101 ataaatagtt taacaaaatt ttaccatact atatgagtat atgttttttt catatagacg
     5161 tcaacttgta agataattta attaagtcga aatatatgta aaaatagtat catttttgtg
     5221 tattcagtaa tattttttta tgataatata tgttattatt atattaaaat acaaacatat
     5281 ttatggaaag gatatgccaa tttagtagtt agattgattg tttggcaggt gggatgagct
     5341 atgataagaa agaaatctga attacaagta gaagtaaaag aaaatttaaa tggtggtata
     5401 ggaaaggtta agcttgaaaa cataatacaa aatgaagagc ttaaaggaaa aggaagacta
     5461 tttaaaagag tagctttacc agtaggaagt tcaattggtg tgcatgacca tacaacagat
     5521 tttgaagtat attatatact aaagggaaaa ggtaaagtat ttgataatgg ggaatttgta
     5581 gaagtaaatg agggtgatgt agtgtataca gctgatggcg aaaaacattc tatagaaaat
     5641 ataggagaag aagatcttga gtttgtagca ctagttttat atatgtaaaa tatggaggag
     5701 gagtcatgac aggcaagata gaagaaatta aggacaagat aaatagattt tgcaactcca
     5761 atttaaatca agaatacaaa gatataagct ttaaaatttt gcaaaacctt ttagataata
     5821 ataaagagat aattcattct tctagagctg atatatggtc ttcagctata ctaaatatag
     5881 ttttagaaca aaatctttta tacaataaga agcatccact acatataaca aaaaaagagt
     5941 tttcaaaaca aataggtgtt tctttaaata ctataaataa taaatcgtct ttaataaaag
     6001 atgtttgtag tattgacttt ttaaatcaag atactatagc tatttctaat gtagaatttt
     6061 gggtaaaaga gactaattcc aaatcaaagt ctgtagattt agatttagaa aagaagaaaa
     6121 tactatataa aagatatatt aagctatcac aagatgcgaa tagtgatatt gaatcaatta
     6181 gatatatgga ggaagctgta aatataggta aaagtatgat tactaaagaa gataaagaac
     6241 ttggattttg gaaaggttta tctaccagag cctatatgat agcacttgaa tcccttgcta
     6301 gaaagttaga aagtattaat aatctaaagg agtccaaaaa agtattagaa tatttaatag
     6361 aaattaatcc tagtgatgag cagggaataa gatacaaatt attaaatgta ttaattagat
     6421 taaatgatag aacttcaata aataaattat ttgaacttta taaagaagaa aaatctgcta
     6481 catggatgta cagtaaagct ctttattact ttaaaaataa aagtatgttt ttggcaaatg
     6541 attctattaa ggttgctaga agtaaaaata agtacgttgg attatatttg ttagattggc
     6601 gaaatgcttt tggtagagaa tttgttacag aggaagaaaa ggcagaagct gtctattact
     6661 atgatgaaaa catagtgatt tggaatgaag ttaaaggttc tatggattgg ctattaaaaa
     6721 aaatgttgga attttcataa aaaaaactac tccttacttt tatataagtc aaaggagtag
     6781 tttttaattt atacatctag ttttaaatct tcaggtttta ttagaccttt ttttctcata
     6841 aattctgaaa tcaacaaagt tacaatagca ggaagtataa aattaagtaa aagtatacca
     6901 acccacatag atatacctcc ttttccagca ctttgcatag cagttactgt tccaaattgt
     6961 ccaaccaatc cacaagtacc cataccagaa ccaataggaa tattctccat tttgaatatt
     7021 gttgtagata atggtccaag taatattgaa gctacagtag gtggtatcca aattttccaa
     7081 ttttttatta tattaggtac ttgcaacata gatgtcccaa gaccttgtgc cacaaggcca
     7141 ccaactccat tttctctaaa gctcataaca gcaaagccta ccatttggca acaacaacct
     7201 acagttgctg caccaccagc aagaccacct aaacttaaca tcatacaaat agctgcacta
     7261 cttataggta atgtaagagc tattccaaca agtgcagaga ctaaagctcc cattattata
     7321 ggttgcatag ttgtagcttg cattattata tttccaaatg aagtcataaa agcagaaaca
     7381 gctggaccaa taagttcagc tacaagtact ccaattagta ttgttacagc aggtgtaacc
     7441 aatatatcta tttttgtttc ttttgaaact aattttccaa cctctacagc aaaaagtgta
     7501 gcgacaaatg ctccaacagg tcctcctaat tcattaccac aagctccaac aagtgcacaa
     7561 gaaaatagta caagtggagg tgcttgaagt ccatacgcaa tagccactgc tatagctgga
     7621 ccagtaacct gtttagcaag agggcttata acttcagtaa aaagagaaat attgaaatta
     7681 tctcctaatg tattgaaaat tgttccaatt aaaagggacg aaaaaagacc gagtgccata
     7741 taacttagtg cgtcaataag atacctttga acagaaattt caatattttt tcttttagca
     7801 aatgatttga aatttctttt tttataattg atttccaatt taaaatcctc ctcatataat
     7861 ataagtataa tttcttttta attaattata taacttttca gctgacaaga caaatgttat
     7921 ttcactaaaa ttagactatt gtaatagtaa gaaattaaca ttaattttag ttttaaattt
     7981 taaatttatg gtttataatt agtataggga tattctgaaa tataataatt ttccagttta
     8041 tcccaatatt taacttaggt agattgctta caaaacaaat gattatatat taggctaaaa
     8101 ggaagtggat agatgatatt tgtaaaaaat aagaaataca gaaaaattgc cataattata
     8161 tctattccta tattgcttgt tgcgcagtac taccttttta aatttggaat acttagacca
     8221 atggtaaagg gagtagaaat tgaaattgta gatggggaat atgttaaaga tatagataaa
     8281 tatgtagtaa aattaaatga aacagttact ttgtctacag gtgaatatat taaggttcct
     8341 tcgtatgcta aaaatccaaa tatttggttt aatgtattgg ataactcagg gactttaaaa
     8401 atagatggaa ataaaatgac agcattaaag gttgggtatt cttctgtggg tatcatgaaa
     8461 aattcaagag tattaaaaaa ggctactata aaagtagtag acccagaggt agaatcacta
     8521 gatatggaga ttaatgggga tttaaaacat gttggtgata gtgctactat agaaagtaca
     8581 atagaagttg attatgataa gtttaaaaaa tcatataagc cagtttataa gagtagcaat
     8641 gaaaatattt taaaaatcaa aggaaataaa gtaagtgcag ttggagttgg taaagctaca
     8701 atatatgcta agtgtggaaa taaagaaatt gagagaacat ttaggattca agctagagtt
     8761 gctgaaataa atatggatga tataaaaata gaagttgacc agaatgaaaa attgaaacca
     8821 gatataataa cttctccgcc tggtttagaa ccaccaaaaa tatattatga gttagtagat
     8881 tcaaaattac ctgtagaaag agcaataagt ttaagtagta atggaaatgt tgtagggctt
     8941 agagaaggta aagatagggt gagaataact tgtggagata aatctaaagt aataacagta
     9001 acagtagtca aagaaactat aacgaatgac tatatacaaa acttggttgg ttcacataaa
     9061 atagtaaaca ataaagttat tattaaactt gaatgggatt atatgaaaga tgtctttgat
     9121 tatgatatat atcttaaaga taagttgtca ggagaatcat ttaagaaaat taaatctata
     9181 actataaatg aaagtgattt aggtaagtct aacaaggtat atgcaactat agaagtagat
     9241 atgaagggta atcaaaatat agattttgat atttatgtca tagggaaaaa ggattcagat
     9301 acaactaagc caagtaatgt aataaatatt accaatggaa atggaaattc aaataatgat
     9361 aataatatta aagatatgag ggtagaaaat atatcagcta atgttgatag agataataat
     9421 gtcattagag ttaactggag tagtatatct cataaggact gtacttacag tatatatgta
     9481 aaaaataata ctaatggaga tggagaattt actctatatc aaagtgatat acagggaaat
     9541 gagcatacta taagtattcc tgaaggagat ttaaatctag atatttatgt tgttgcaaat
     9601 tataatggaa atagctctca aaatagtgat gttataaatg taagatagat ttgtatacaa
     9661 attgaaagtg ttgagtttat ttaaatatta aattaagaaa aaggatagta tagggtaatt
     9721 atactatcct tttttgttac aatatatagg tttaaaatct ctagtaatat aaattataaa
     9781 aagtcttatt tacacaaatc tatagatatt ttttttatta atcaatattg tcttaaattg
     9841 aacttagtgc ggcttctatt gcatgtatta ctgtcgtatc aaatagtgga atagaagtgt
     9901 cattttgttt aattaaaaga cctatttctg tacatcctaa tataacagcc tctgcacctt
     9961 gtttgccaag cttatctata atagataaga aagctttttt tgaagattca gatataattc
    10021 ctaagcaaag ttcattaaat attgtgtcat ttactattat acggtcttct tcatttggaa
    10081 ttaagacttc aattccatta ttaataatta catttttata aaaatcttgt tccattgtat
    10141 atttagtacc taataaggcc acttttttta tttctttttc ccttaatacc gtagcagtta
    10201 catctgctat gtgaagtaat ggaatatgta tactttcttg tattttatct gataccttat
    10261 gcatagtatt agtacagata acaataaaat ctgcccctgc ctcttgtaat ttaattgcag
    10321 catcagctaa aattttggcg cttttttccc aatttccact agattggcat tcttcaattt
    10381 cttgaaaatc tacactgtaa agtatacatt tagaagagtg tagtccacct aatctttctt
    10441 ttattactgt attaataact tgataatatg taatggtact ttcccaactc ataccaccaa
    10501 ttaagcctat agtcttcata ttatacctct cttatttatt tattttgaaa tagctattta
    10561 caaatacaag tatagcatct aaaatagaag catacaatga taaaattgta tggagtacaa
    10621 ttataattaa gaggagtaat tatatgccaa taaattcttt tgaaaactat cctatgacat
    10681 ggaaaccaaa attagataac cgaaaaccac caatatataa aactttagca atgttattag
    10741 aagaagatat taaaagagga aatttaaatc ctggagataa actgcctcca caacgggaat
    10801 tagcagattt tctagattta aacttaagta caataacacg agcatttaaa ctttgcgaag
    10861 aaaagggact aatatgtgca aaagtgggaa agggaacatt catttcttct gatgtaaatg
    10921 taagtaatac attattatat caaacggaat caaaagacat aattgaatta gggacagttc
    10981 atcctccgta tgagcaaaat acatatatta ttgactttat taaaaatgta ctaaaacaac
    11041 cagaaatgga taggttttta caatacatgt ctccatctgg cacatatatg caaaaaaagt
    11101 caattgcaaa atggatagaa agaaataatg tatacaccag tgaagaaaat atattattat
    11161 ctacaggagg gcaaaatgct atatgtgcaa ctttactagg attatttaaa gctggtgata
    11221 gaattgctac agattcttta agtttttctg gaattaagtc aatagctaaa atgataggta
    11281 ttcaattagt cccaattcct caagaaaata atgaaatatc gattgaatat cttgaaagtt
    11341 attgtaaaaa tgaaaatata aaaggcatat atcttatacc agattatcat aatccaacga
    11401 cacatactat gtctgattta tctcgtaaaa agatagccaa gatagcaaag caatataata
    11461 taataattat tgaagatgct ataaatagca tttttagaga tggtatacaa actcccatat
    11521 tttctttagc tagtgataat actatatata tttttagtac atctaaattt ttatgtgctg
    11581 gtcttagagt agcatttgta gttgctccaa aacgatatat agaaaattta gaaaatgcac
    11641 tatataatat gaatctaatg gtttctccat ttacagcaga aatagtacac cgtttattat
    11701 attctcctat tatagataaa attatagaag agaaaagaga tgcaattata gaaagaaatg
    11761 aagttgctga taaagttttg tcagattata atttgattgg tgataagaat tgcagttttc
    11821 gttggttact tcttcctgat gaattggatg gaaaatcatt tgaaatttct gcaaaaaatc
    11881 taggagttca agtatattgt gctgaacgat tttcagtagg aaactctaca gttccaaaag
    11941 cagttcgtat ttgtgtaaca gctccaaaag atgtagaaga attagaaaaa gggcttaata
    12001 taataaagtc acttcttaag agttaaattt attttactat tatttaatta aat
//
LOCUS       DAGSWI010000127        11689 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.128, whole genome shotgun sequence.
ACCESSION   DAGSWI010000127 DAGSWI010000000
VERSION     DAGSWI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.128"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            459..596
                     /locus_tag="MJF80_003080"
     CDS             459..596
                     /locus_tag="MJF80_003080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281720.1"
                     /translation="MGNSDCSKMLAFYEIMKMLETIDEAKFYNCKEEIKSEFKHIKKE
                     D"
     gene            complement(624..1016)
                     /locus_tag="MJF80_003081"
     CDS             complement(624..1016)
                     /locus_tag="MJF80_003081"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281721.1"
                     /translation="MYKDESNIFSKRLREEREELGLMQKEMANKLSLPANTYNGYETG
                     KRSPALDVVRHIADTLDISTDYLLGRTNIKINISNITEKELIKKLNPSDDMKEILDIF
                     SELDDDSKNKALKIAKLFLEEQNTKNKE"
     gene            1204..1398
                     /locus_tag="MJF80_003082"
     CDS             1204..1398
                     /locus_tag="MJF80_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008422227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281722.1"
                     /translation="MKNYILKSLRVRQGLRQRDLAKILGMNPSTYSSKENGDRRFTVS
                     EAIKISDFFNTDLRDIFLNK"
     gene            1434..2225
                     /locus_tag="MJF80_003083"
     CDS             1434..2225
                     /locus_tag="MJF80_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBZ0281723.1"
                     /translation="MNNLQVIERNNKRVLTTQQLADVYETDARNISNNFNNNKDRFIE
                     GKHYFCLQGDDLRIFKRGSYDIGIAPNVNKLYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQQKLTCIEDVLIESLKEMKDLRLQVNQANNIALEAKTEVKTIKEVVSLNA
                     TDWRKDTQQLIARIAKKQGGFEHINMLRRESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            2287..2454
                     /locus_tag="MJF80_003084"
     CDS             2287..2454
                     /locus_tag="MJF80_003084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281724.1"
                     /translation="MENKKIEEIKNALIGVNQIEWSLVKSFIDMYFSKKAAKLEIDDL
                     DNFDLYIKRKF"
     gene            complement(2451..2690)
                     /locus_tag="MJF80_003085"
     CDS             complement(2451..2690)
                     /locus_tag="MJF80_003085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281725.1"
                     /translation="MTKIYACLAGNWVNLCDDPECKMGSNHSSPYIWWEEGADVWSPS
                     NKEEEHTMYQQDYVHIYYKGVDYRIHPIFIQIASN"
     gene            2766..2963
                     /locus_tag="MJF80_003086"
     CDS             2766..2963
                     /locus_tag="MJF80_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281726.1"
                     /translation="MLIGDNISQILRKRNIKPYKLAKELGIDESGLYKIIRNEKKNPT
                     INTLIKIADYLDVTLDELVGR"
     gene            3002..3211
                     /locus_tag="MJF80_003087"
     CDS             3002..3211
                     /locus_tag="MJF80_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281727.1"
                     /translation="MAKAVAKEPLFYRAKDVAKFLDICEATAYKIIAELNEELEKEGF
                     KTFSGRVSVAYFKERYCYKPRKGVI"
     gene            3212..3352
                     /locus_tag="MJF80_003088"
     CDS             3212..3352
                     /locus_tag="MJF80_003088"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281728.1"
                     /translation="MNVRVLIAYIQFCNDKQIKASFEGLRKYNRDIELVNLYLPAHLK
                     IK"
     gene            3384..3524
                     /locus_tag="MJF80_003089"
     CDS             3384..3524
                     /locus_tag="MJF80_003089"
                     /inference="COORDINATES: protein motif:HMM:NF015807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBZ0281729.1"
                     /translation="MQREQTTIRLPKELKEKLEKQASKKGRSFNSILLSILQEFIQNP
                     NV"
     gene            complement(3502..3690)
                     /locus_tag="MJF80_003090"
     CDS             complement(3502..3690)
                     /locus_tag="MJF80_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HBZ0281730.1"
                     /translation="MAKMGTYKNPHFAIRIPKEKLDKLKYIAEYNARSANKEIEFLVT
                     KHIEMFEKEHGPIHLDSE"
     gene            3846..4004
                     /locus_tag="MJF80_003091"
     CDS             3846..4004
                     /locus_tag="MJF80_003091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281731.1"
                     /translation="MLANRVRTGLRIPYDLNTKLILIAEERGMSKNSLILQILWDYIK
                     EIENKEVK"
     gene            4004..4231
                     /locus_tag="MJF80_003092"
     CDS             4004..4231
                     /locus_tag="MJF80_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281732.1"
                     /translation="MVELVKEFDLQTIKVGNAVKVNCKRFGFEIDCIVVVATEKELNL
                     AYFDEGRGCMEYQALITEDIQDGDYEIKILS"
     gene            4426..5316
                     /locus_tag="MJF80_003093"
     CDS             4426..5316
                     /locus_tag="MJF80_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBZ0281733.1"
                     /translation="MDWLKNRQLGIGGSDASAVAGLNPWKTSVQVYIEKKEEIPIETK
                     SFRMELGNRLEGLVAELFTEETGLKVRNVNGMLKNEKYPFAIANIDRAIVGEKAFLEC
                     KTTNSFSIKEWENGVPLHYEIQCLHYMAVTGATHCYIAALLGNEKFVWHKINRDNEVI
                     KNLMKIESEFWEENVLKDILPIPDGSDAYSEFLKTRYKNSVKEKIELNLLEDGISKLK
                     RYDDIVLQMKELKGEKQLIEQEIQSEMREFELATLGGRIITWKGATKRSIDTKRLREE
                     MPDIAEKYTNISSYRTFKIK"
     gene            5329..6162
                     /locus_tag="MJF80_003094"
     CDS             5329..6162
                     /locus_tag="MJF80_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBZ0281734.1"
                     /translation="MASEKAKGALEKKVSGANTVKVSPSKGMEQLMNKMASQIKKALP
                     SMVSSERFQRVALTAFSNNPRLQSCEPMSFIAAMMESAQLGLEPNTPLGQAYLIPYGN
                     KVQFQIGYKGLLELAQRSGKIKTIYAHKIRENDKFEIKYGLHQDLVHEPKLNGDRGEI
                     IGYYAVYHLDTGGHSFSFMTKEEIIEFAKSKSKSYSSGPWQTDFDSMAKKTVIKQLLK
                     YAPLSIELQKAMVGDETIKSEIDEDMSMVVDESESLEVDFEVKENMDGKVSVEEAINV
                     D"
     gene            6177..7115
                     /locus_tag="MJF80_003095"
     CDS             6177..7115
                     /locus_tag="MJF80_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0281735.1"
                     /translation="MNEDKSVIEKLNILSGGYGLMPRIIARDRWLTVGARMLYSYLTS
                     FAGNDGTCFPSRDLICYELDISKDTFTKYKKELEMSGYIRVHKNKSKQGKMQNNIYEI
                     VFDRTYIDECISKRGLKEEKKKKKPCTKKQDTEPYPKNVDMEPCPTFPDTTQPDTENM
                     DTNSNSINSNSINNMYIVKQPVDNFLKEFKKLYEENIGVIYPVTAEWLLEVSNEVDIR
                     VFKRAIEICAEKMNMNLAYLKGILKKWKDANITTYEQLESYRLQQENKKTKKVVNNHV
                     SKNKFANFEQTFTKYSENELDDIIKKSQKEKFGVGS"
     gene            7135..7533
                     /locus_tag="MJF80_003096"
     CDS             7135..7533
                     /locus_tag="MJF80_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281736.1"
                     /translation="MELKFREWNKNGKEMYSYDEMVCYSKNLLREWVYSGVYLPTSNE
                     NFEVMIYTGLKDCIRKEIYEGDIVSYILSFEEFIGEVKFEEGFFVIDNEVLGECVGLF
                     HEIAVVKVIGNIYENPEMLEKIRKPKVLEV"
     gene            7536..7808
                     /locus_tag="MJF80_003097"
     CDS             7536..7808
                     /locus_tag="MJF80_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281737.1"
                     /translation="MKHECKKLFLECDKGSFEINDTRIGEVMFESTEIDNPFERVKYE
                     GKATFEIVSGWEYLQREMLWLKILHLSAVVAKIMQYKMLGILIREM"
     gene            7813..8193
                     /locus_tag="MJF80_003098"
     CDS             7813..8193
                     /locus_tag="MJF80_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281738.1"
                     /translation="MAKIWMDAGTFLEKTIDIEDMFELNLRAIRKRNEKIKNEIEKQI
                     KYEDSEIKTTNRGGPGKIIKIFNINTGEVKILKSAEEASRYIKVSRSYASYLARENKS
                     TEDGWKAEYIQGATDGISKCGTSN"
     gene            8418..8594
                     /locus_tag="MJF80_003099"
     CDS             8418..8594
                     /locus_tag="MJF80_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281739.1"
                     /translation="MDDRLEIINASVNYIQMICESSNIAIIAERGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
     gene            8708..9397
                     /locus_tag="MJF80_003100"
     CDS             8708..9397
                     /locus_tag="MJF80_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281740.1"
                     /translation="MDLNKIMNDALVELEENGFVEEVVKKQLAETIKRVVNEVFGNWG
                     DFSKKIEKHLSENININLDKLDIQKYNVLVATTVKEKIDMTMKVEAIEHLKRNLDNML
                     VGAEKEYKMSELLEILKMDKYDIDEYEGYEDCITFILDENKYGSGWIEIDKEPNKSKY
                     RCEHSILLRKDGTIASYRYENREISSKDIMSGFDNFGDLLFKIFAHGSKIILDLGNDI
                     EDYDLTNGEDY"
     gene            9415..9636
                     /locus_tag="MJF80_003101"
     CDS             9415..9636
                     /locus_tag="MJF80_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281741.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSIENLKKKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMAEYFEDEGVEV"
     gene            9633..10637
                     /locus_tag="MJF80_003102"
     CDS             9633..10637
                     /locus_tag="MJF80_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBZ0281742.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDIEAFFQKKISKVSR
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNNSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDKYHLRVLDKDAEKEEANSKFVTEFLNATKIKDDKYKTKKFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLREKHEIDINDFVDKTIKDDKLKDSFKEHMEEKGLVEGFSI
                     DKKWVDKKLKKRNIKTDNGFEIKGNLTDFEDPMKYTVRQNQNGSIDIVIKNVTFYEEK
                     "
     gene            10719..11048
                     /locus_tag="MJF80_003103"
     CDS             10719..11048
                     /locus_tag="MJF80_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281743.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNDTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            11085..11456
                     /locus_tag="MJF80_003104"
     CDS             11085..11456
                     /locus_tag="MJF80_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281744.1"
                     /translation="MFNIYKVKIKTKRTLEQVRNQSVDFEYSEKGLKNTLKYYNLIDD
                     LKVIVVKFGDEYCLANYNEEDRKIIMEAHYLLEQDEYTGCYINEYERFKKDWENGNCD
                     GEACMVFSDDEIEIIEKLREG"
     gene            11459..11641
                     /locus_tag="MJF80_003105"
     CDS             11459..11641
                     /locus_tag="MJF80_003105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281745.1"
                     /translation="MNKRIKMKKRLIHKKCDERCVNYDFVISNNLITCNVCIGCKYKE
                     NMDKVCEENYKKLRSK"
BASE COUNT         4967 a         1100 c         2179 g         3443 t
ORIGIN      
        1 tttaaaagct tataaatata aagtaaataa tttgtaaatt aaatctaaga ataatgtaaa
       61 aaatgcaata aaaaaataaa aattaaaatt atgaaaaata aagctagtcg actaactaaa
      121 aatagaaata tacagataat ctaaaatact agaaataaaa aaatagaagt gaatttttaa
      181 aatcaaaact aaaatctcta tattagtcaa gttgcaagat aatataaatt tatatataat
      241 atatttacaa agaaaatttt agaaatttta aatattatat ttgttgaaat aaatacatca
      301 agtctataag ttaaggaatt tttataccat tttttacgaa cataagttct gaaaagggat
      361 ataaaatata atattataaa aagaaggtga ttggctaaat attttacata tttaaggggc
      421 aaggaataaa tattataaat tataagaggg tgacgcaagt gggaaattca gattgttcta
      481 aaatgttggc attttatgaa attatgaaaa tgttagagac tattgatgaa gctaaatttt
      541 ataattgtaa agaagaaata aagtcagagt ttaagcatat aaaaaaagag gattgaatcc
      601 tcttttttta tatattttta tgtttattct ttatttttag tattttgctc ctccaaaaaa
      661 agttttgcta tctttaaagc tttattttta gaatcatcat caagttcact aaaaatatca
      721 agtatttctt tcatgtcatc agatggattt agctttttaa taagttcttt ttctgtgata
      781 ttagaaatat taattttgat attagttcta cctaataaat agtcagttga tatatctaaa
      841 gtatcagcta tatgtctcac aacatcaagc gctgggcttc ttttaccagt ttcatatccg
      901 ttataagtgt ttgctggtaa acttaattta ttagccatct ctttttgcat taatccgagt
      961 tcttctctct cttctcttaa tcttttagaa aatatattgc tttcatcttt atacattgta
     1021 attgtccccc tcttaaaaac tataacgaat gtaattactc ttattctact acaaaatacg
     1081 cattttgtga ttttaattcg caaaaaggaa gaaaaaaaca aaaaacgtat tgacaattca
     1141 caaaacggag tatattatat aaatatagaa acttcaaatt gagaataaaa ggagtgatat
     1201 atattgaaaa attatatatt gaaatctctt agagtaagac aaggattgag acaaagagat
     1261 ttagcaaaaa tattaggaat gaatcccagt acttattcaa gtaaagaaaa tggcgatagg
     1321 cgattcacag taagtgaagc aataaaaata tcagattttt ttaatacaga tttgagagat
     1381 atttttttaa ataaataatt cgcgaaatgt gaataaaaag gaggtagata aacatgaata
     1441 acttacaagt aatagaaaga aacaacaaaa gagttttaac tacacaacaa ctagcagatg
     1501 tatatgaaac agatgcaaga aatataagca acaattttaa caataacaaa gatagattta
     1561 ttgaaggtaa acattatttt tgtctgcaag gtgatgattt aaggatattt aaacgcggtt
     1621 catatgatat aggaattgca cctaatgtaa acaaacttta cctttggact gaaagaggag
     1681 caaacagaca ctgtaaaata ttagatactg ataaagcttg ggaacagttt gataacttag
     1741 aagaaacata tttcaaagtt aagcaacaga agctaacttg tatagaagat gtattaatag
     1801 aaagtttaaa agaaatgaaa gatttaagac ttcaagttaa ccaagctaac aatatagcat
     1861 tagaagcaaa aacagaggtt aaaacaataa aagaagttgt ttcattaaat gctactgact
     1921 ggagaaaaga tacgcaacaa ttaattgcaa ggatagcaaa gaaacaaggt ggatttgaac
     1981 atataaacat gcttaggaga gaaagctacg aattactaaa taagcgattt ggagttgacc
     2041 tacatagaag attaatcaat aaaagaagaa agatggcaga agaaggtgta tctgaatcta
     2101 aaagagagaa agttaacaat ttagatgtaa tacaagatga taagaaactc atagaagggt
     2161 atgtggctat tgtaaaagat atggctttaa aatatggaat atcaagtgat ttaagcaaaa
     2221 attaagttaa atcacaacag cactttgaaa actaaataca gaatattttg aaaaggagtg
     2281 gttaaattgg aaaataaaaa aatagaagaa ataaaaaatg cattaatagg ggtgaatcag
     2341 atagagtggt ctttagtaaa atcttttatt gatatgtatt ttagtaaaaa agccgccaaa
     2401 cttgaaattg acgacctaga taattttgat ttgtatataa aacgaaaatt ttaattacta
     2461 gcaatttgta taaagattgg atggattcta tagtcaacac ccttatagta aatatgaaca
     2521 taatcttgct gatacatagt atgttcttct tctttattag aaggactcca tacatcagca
     2581 ccttcttccc accatatgta aggcgaagaa tggttactac ccattttaca ttctgggtca
     2641 tcacatagat taacccaatt accagctaaa caagcatata ttttagtcat aatatcacca
     2701 actttcatat aaatttggaa tatattccat atttatatta taccatgtag aacgaaggtg
     2761 attaagtgtt aataggcgac aatataagtc aaatactaag aaaaagaaat ataaaacctt
     2821 ataaattagc aaaagaatta ggaatagatg aaagtggatt atacaaaatt ataagaaatg
     2881 aaaagaaaaa tccaaccata aacactctaa taaaaatagc tgattattta gatgttacat
     2941 tagacgaatt agttggaaga tagaaattaa atacagaata ttttgaaagt aggtgtattg
     3001 tatggcaaaa gcagtagcta aagaaccatt attttataga gcaaaagatg tagctaaatt
     3061 tttagatata tgcgaagcaa ctgcatacaa aataattgca gaattaaatg aagaattaga
     3121 aaaagaaggt tttaagactt tttctggcag ggtatcagtt gcttatttta aagaaagata
     3181 ttgttataag ccaagaaagg gggtgattta gttgaatgta agagtattaa tagcttatat
     3241 acagttttgt aatgataagc aaataaaagc aagttttgaa ggtcttagaa aatacaacag
     3301 agatatagag ttagttaact tgtatttacc agcacattta aaaataaagt aagctataaa
     3361 taattaaaga aaaaggtgat tagatgcaaa gagagcagac aacaatacgc ctgcctaaag
     3421 aacttaaaga aaaattagaa aagcaggcga gtaaaaaagg aagaagtttt aatagtattt
     3481 tattaagcat attacaagaa tttattcaga atccaaatgt ataggaccat gttctttttc
     3541 aaacatttct atatgtttag tgacaagaaa ttctatttct ttgttagctg aacgagcatt
     3601 atactcagct atatatttaa gtttatcaag tttttctttt ggtattctaa ttgcaaaatg
     3661 aggattttta tatgtaccca ttttagccat aatatcaact cctttgtgta gtctatttgg
     3721 caacaattat atcacaaaaa taaatacaaa aaaatatata ggcaacatat agacaacata
     3781 ataataaagt gttatacttt gtattaaaga taggcaacac atagataaca atataggagg
     3841 aaattatgtt ggcaaataga gtaagaactg gattaagaat cccatatgat ttaaatacaa
     3901 aattaatatt aatagcagaa gaaagaggaa tgtcaaaaaa ttctttaata ttacaaatac
     3961 tttgggatta cataaaagaa atagaaaata aggaggtcaa ataatggttg aattggtaaa
     4021 agaatttgat ttacaaacaa ttaaagtagg aaatgcagta aaagtaaatt gcaaaagatt
     4081 tggttttgaa attgattgta tagtagtagt agcaacagaa aaagaattaa atttagcata
     4141 ctttgatgaa ggtagaggct gtatggagta tcaagcctta ataacagaag atattcaaga
     4201 tggtgattat gagattaaaa ttttatctta ggaggaaata aaatggtagg tttatttgca
     4261 atatgtttag caggattatt tcaataaaaa aagtgctggt caaagtaacc aacacataca
     4321 aaaaattcaa cttatttagg aggataccat aaaatgaata aaatttcaag tcgtagaaaa
     4381 tatttagatg cttttatagt aactgatact aagaacatag ataaaattga ttggcttaaa
     4441 aatagacaat taggaatagg gggtagtgat gcgtcagcag tagcaggatt aaatccctgg
     4501 aaaacttctg ttcaagtata tatagaaaag aaagaagaaa taccaataga aactaaaagt
     4561 ttcagaatgg aattaggcaa tagattagaa ggattagttg cagaactttt tacagaagaa
     4621 actggtctta aggtccgtaa tgtaaatgga atgttgaaaa atgaaaagta tccttttgca
     4681 atagctaata tagacagagc tatagtagga gaaaaagcat ttttagaatg taagacaaca
     4741 aatagttttt ctataaaaga atgggaaaat ggagttccac ttcattatga aatacaatgc
     4801 ttacactata tggctgtcac aggagctaca cattgttata tagcagcact tcttggaaat
     4861 gaaaagtttg tatggcacaa gataaatagg gataatgaag taattaaaaa tctaatgaaa
     4921 atagagagtg aattttggga agaaaatgta ttaaaagaca ttttaccaat tcctgatggt
     4981 tcagatgctt atagtgagtt tctgaaaaca aggtataaaa actcagtaaa agagaaaata
     5041 gaactaaatc tacttgaaga tggtatatca aagttaaaaa gatatgatga tatagtttta
     5101 caaatgaaag aactaaaagg agagaaacag ctaatagaac aagaaataca aagtgaaatg
     5161 agagagtttg agttagctac attaggcgga agaataataa cttggaaagg agctactaaa
     5221 aggtccattg ataccaagag attaagagaa gaaatgcctg atatagcaga aaaatataca
     5281 aatataagtt catacagaac attcaaaata aaataggggg taatatatat ggctagtgaa
     5341 aaagcaaaag gagcattaga aaagaaagtt tcaggagcaa atacagtcaa ggtaagtcca
     5401 agtaaaggta tggagcaact tatgaataaa atggcaagtc agataaaaaa agctttacct
     5461 agtatggttt caagcgagag atttcaaaga gttgccctaa cagcttttag taataatcca
     5521 aggttacaat catgtgaacc tatgagtttt atagcagcaa tgatggaatc agctcaatta
     5581 ggtcttgagc ctaatacgcc tttaggtcaa gcatatttga taccatatgg aaataaagtg
     5641 caattccaaa ttgggtataa aggtctttta gaattagcac aaagaagtgg aaagataaaa
     5701 actatatatg ctcataaaat aagagaaaac gataaatttg agattaaata tgggcttcat
     5761 caagacttag ttcatgaacc taaattaaat ggtgatagag gggaaataat tggatattat
     5821 gcagtatatc atttggatac aggaggacat agtttttctt ttatgactaa agaggaaatt
     5881 atagaatttg caaagagtaa aagtaaaagt tatagtagtg gaccatggca aacagatttt
     5941 gattcaatgg ctaaaaagac agttataaaa cagttattaa aatatgcacc acttagtata
     6001 gaattacaaa aagctatggt aggtgatgaa acaataaaat ctgaaataga tgaagatatg
     6061 agcatggtcg tagatgaaag cgaaagttta gaagttgatt tcgaagtaaa agaaaatatg
     6121 gatggtaaag ttagtgtgga agaagctata aatgttgatt aagtaggtga ggcaccttga
     6181 atgaagataa gtcagttata gagaaattaa atatattaag tggtggatac ggtcttatgc
     6241 caagaataat agcaagagat aggtggttga cagttggcgc tagaatgctg tattcatatc
     6301 taactagttt tgcagggaat gatggaacat gttttccatc tagggattta atttgttatg
     6361 aactagatat atcaaaagac acatttacaa agtacaaaaa agagctagag atgagtggct
     6421 atataagggt tcataagaat aaatccaaac aaggcaagat gcaaaacaat atatatgaaa
     6481 tagtatttga tagaacttat atagatgaat gtatttctaa gagaggttta aaagaggaga
     6541 aaaagaagaa aaagccatgt actaaaaagc aagacacgga accgtatcct aaaaatgtag
     6601 acatggaacc atgtcctact tttccggaca cgactcagcc ggacacggaa aatatggaca
     6661 ctaatagtaa cagtattaat agtaacagta ttaataatat gtatatagta aagcaacctg
     6721 tggataactt tttaaaagaa tttaagaagc tgtatgaaga aaatatagga gtaatatatc
     6781 cagttacagc tgaatggtta ttagaagtat ctaatgaagt agatataaga gtatttaaaa
     6841 gagctataga gatatgtgct gaaaagatga atatgaatct agcatactta aaaggtatcc
     6901 ttaaaaaatg gaaggatgca aatattacta catatgagca actggaatca tatagattac
     6961 agcaagaaaa taagaaaaca aaaaaagtag ttaataacca tgtgagtaaa aataagtttg
     7021 ctaactttga acaaacattt actaagtatt cagagaatga gcttgatgac attattaaga
     7081 aaagccaaaa agagaagttt ggagtaggaa gttaaaatat tggagggatg aaagatggag
     7141 ttaaaattta gagaatggaa taaaaatggt aaagaaatgt atagttatga tgaaatggtg
     7201 tgctattcta aaaatttgct tagagagtgg gtttatagtg gtgtttattt accaacaagc
     7261 aatgaaaatt ttgaggttat gatatacaca ggtttgaagg attgtattag aaaagaaatc
     7321 tatgagggtg atattgtttc atacatctta tcatttgaag aatttatagg agaggtaaaa
     7381 tttgaagaag gtttctttgt aatagataat gaagtgctag gagaatgcgt tgggttattt
     7441 catgaaattg cagtggttaa agttattggc aatatatatg aaaatcctga gatgttagaa
     7501 aagataagga aacctaaagt gttggaggtc taaaagtgaa acatgagtgt aagaaacttt
     7561 tcttggaatg tgataaggga agttttgaga taaatgatac aagaattgga gaagtaatgt
     7621 tcgagagtac agaaatagac aatccgtttg agagagtaaa atatgaaggt aaagctactt
     7681 ttgaaatagt atctggatgg gagtatctac aaagagaaat gttgtggctt aagatattgc
     7741 atttatcagc agttgtagca aaaataatgc aatataaaat gttaggtatt ttaataaggg
     7801 agatgtaata aaatggctaa aatttggatg gatgcaggaa cgtttttaga aaaaactatt
     7861 gatatagaag atatgtttga acttaattta agagcaataa gaaagagaaa tgaaaaaata
     7921 aaaaatgaaa ttgagaaaca aataaaatat gaagattcag aaataaaaac tacaaatcgt
     7981 ggtggaccag gaaaaattat aaaaattttc aatataaata ctggagaggt aaagattctt
     8041 aagagtgctg aagaagcaag tagatatata aaggttagtc gtagctatgc aagttattta
     8101 gctagagaaa ataaatcaac tgaggatggt tggaaagcag agtatattca aggagcgaca
     8161 gatggtatta gcaaatgtgg aacaagtaat taagttagct gaaaagatat taaataagaa
     8221 aaagtgttct gttaataaag ctattgatat agctataaaa atattaagta ggtatgagtg
     8281 tgaggggatg ttagaaaaat gaatatatta gctattacaa tgcttataat aggaagtttt
     8341 atagctggta gagtttatga gtatagattg aatctaaaag agtgtgaaaa ttgcgacaat
     8401 aaaagaggtg tataagaatg gatgatagat tagaaattat aaatgcttct gtaaattata
     8461 tacagatgat atgtgaaagt tcaaatatag ctattatagc agagcgagga agagttagaa
     8521 tattagattt agaaactaaa gaaaaatatg atttattaaa aaataaactc gaggaaatgt
     8581 tagaagaaat ataagtgaaa atatctaatt aagacagttt agagagttgc aaaatgtctt
     8641 ttggtataaa ttattgttga agtgttttgt gactctcaaa aatgaaaata gagaggggaa
     8701 taatataatg gatttaaata aaattatgaa tgatgcacta gtagagttag aggaaaatgg
     8761 ctttgtagag gaagtagtta aaaaacaatt ggcagaaaca ataaaaagag ttgtgaatga
     8821 ggtttttgga aattggggtg attttagtaa aaaaattgaa aaacatctaa gtgaaaatat
     8881 aaatataaat ttagataaat tagatataca aaaatataat gtgctcgtag caactacagt
     8941 aaaagaaaag attgatatga caatgaaggt agaagctatt gaacacttaa aaagaaattt
     9001 agataacatg cttgtaggtg ctgaaaaaga atataaaatg agcgaattgt tggaaatatt
     9061 aaaaatggat aaatatgata ttgatgaata tgaaggctat gaagattgca taacatttat
     9121 tcttgatgaa aataaatatg gttcgggatg gattgaaatt gataaagaac caaataaaag
     9181 taaatacaga tgcgaacact ccatattgtt gagaaaagat ggaactattg catcatatag
     9241 atacgagaat agagaaatat catctaaaga tataatgagt ggatttgata attttggaga
     9301 tttgctattt aaaatatttg ctcatgggtc aaaaataatt ttggatttag gaaatgatat
     9361 agaagattat gatttaacaa atggagaaga ttattaaaat aggggatgga acaaatgtca
     9421 aagtatgtat tgagatggca aaaaggatta ttactagatg aacgcaagat aaattattca
     9481 tgtggaagta tagaaaattt aaaaaagaaa gctgaattat tagctaaaga tgacaagata
     9541 ctgcttgtaa caatagataa agttgaagaa gttataaaag atactagaag ccaaaaaatg
     9601 gctgaatatt ttgaggatga aggggtggaa gtatgataat acacaaattt ataatacatg
     9661 ttttagataa gaatagcgac acaccaatac taaatgattt tgagggtaga gtcagccaag
     9721 atattgaagc tttctttcaa aagaaaataa gcaaagtatc aagagataat gacatcagaa
     9781 cagcagtatt taatgactat agtaacaatc taattaagaa gtgttgtgaa caaattattt
     9841 atgatgaaag ttcattttta aataactcta aagagattgc agcttattta tttgatgtta
     9901 tgaaattgaa tgctacatta gaatcttgcg acttagcaat ttgtttatac tctcaaaaag
     9961 atgaaaagaa agttgctata ttaaagcttg attacaataa ttcgtatact cattcaattg
    10021 agtttaaaga tgataaattt aatatacaga tgtctaaaaa tgaaattaat atacaagaga
    10081 ctaagacggt taaaattgct gctttggttg gattgagtgg aatgaatgac aaatatcatc
    10141 ttagggtttt agacaaggat gcagagaagg aagaagctaa ttctaagttt gttacagagt
    10201 tcttaaatgc cactaagata aaagatgata agtataagac taagaagttc aaaaatacag
    10261 ctgagaattg gataactaat gctcttagta atgatataaa acaagcagag gatgtaagaa
    10321 gtatattaaa ttatactttg agagaaaagc atgaaattga tataaatgat tttgttgata
    10381 aaacaattaa agatgataag ttaaaagata gttttaaaga acatatggaa gaaaaaggtc
    10441 ttgttgaagg atttagtata gataaaaaat gggttgataa aaagcttaaa aagagaaata
    10501 taaaaactga caatggcttt gaaataaaag gtaacttaac tgattttgag gacccaatga
    10561 aatatacagt aagacaaaat caaaatgggt ctatagatat agttattaag aatgtaacat
    10621 tttatgagga aaagtaggta ctcatgtgac tattggctag agaaggagaa gtaaataata
    10681 agaggatgta aatttaaact agttaggagg aataacttat gaagattttt ttattgacta
    10741 tactgctaat aattatttgt atattagcaa attatgtgaa aaatcgcata tataaaaaat
    10801 ctataaataa tctaaaatat aaatattctg taggggaaaa gattatatat catcaaataa
    10861 actgttacta taacagaatg gttggttgtg aaattttaga aaaatgttat agtacgaaat
    10921 ttagaaaaag aaataccccg ctttataaag taaaagcata tgtaggtgat aacgatacaa
    10981 catgggttat accagagtgg agaattgaat gtcttgctac gacttatgga gaatttccta
    11041 aatattaaat aataaaaatt ggctggagaa ggagattgta aattatgttt aacatctata
    11101 aagtgaaaat aaagactaaa agaacattgg agcaggtaag aaatcaaagc gtagactttg
    11161 agtattcaga aaaaggatta aaaaatactc tgaaatacta taacttgatt gatgatttaa
    11221 aagtaatagt agttaaattt ggagatgaat attgtctagc taattacaat gaagaagata
    11281 gaaaaataat aatggaagca cattatcttt tagagcagga tgaatatact ggatgttata
    11341 taaatgaata tgaacgattt aaaaaagatt gggaaaatgg taattgtgat ggggaagcct
    11401 gtatggtatt ttcagatgat gaaattgaga taattgagaa gctaagggag ggttaaatat
    11461 gaataaaaga attaaaatga aaaaaagatt aattcataaa aagtgtgatg aaagatgtgt
    11521 caactatgac tttgtaatta gcaataacct tataacttgt aatgtgtgta taggatgcaa
    11581 atacaaagaa aatatggata aagtatgtga agagaactat aagaaattaa gaagtaaata
    11641 gaataaaata gtcaaggtaa gtttgtgaat gaaactagaa tgttatagg
//
LOCUS       DAGSWI010000128        11570 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.129, whole genome shotgun sequence.
ACCESSION   DAGSWI010000128 DAGSWI010000000
VERSION     DAGSWI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11570
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.129"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     regulatory      235..339
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            549..3023
                     /gene="gcvPA"
                     /locus_tag="MJF80_003106"
     CDS             549..3023
                     /gene="gcvPA"
                     /locus_tag="MJF80_003106"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBZ0281746.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSILRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYIATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            3023..4480
                     /gene="gcvPB"
                     /locus_tag="MJF80_003107"
     CDS             3023..4480
                     /gene="gcvPB"
                     /locus_tag="MJF80_003107"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBZ0281747.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIYDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            4903..7260
                     /locus_tag="MJF80_003108"
     CDS             4903..7260
                     /locus_tag="MJF80_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBZ0281748.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(7346..7663)
                     /locus_tag="MJF80_003109"
     CDS             complement(7346..7663)
                     /locus_tag="MJF80_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBZ0281749.1"
                     /translation="MSEFKNITAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            complement(8247..9518)
                     /locus_tag="MJF80_003110"
     CDS             complement(8247..9518)
                     /locus_tag="MJF80_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBZ0281750.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIMLEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
     repeat_region   10165..11499
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10165..10193
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
BASE COUNT         4434 a         1300 c         1860 g         3976 t
ORIGIN      
        1 atctcaatat aaatttacat ttggttcttt gtgtgttatt ttaataataa attaagtgaa
       61 aatgttattt tacattaata tataatatct ttgcataaga gtgaataata taaaagagaa
      121 ataattaata ataaaaactt aacaaaaaaa ttaatacata aaaaaaaata aatataatgt
      181 gttgacattt aattgaaata ttcatacaat aaaattgaaa ataaatattt ccaaatgaga
      241 ttagcgagag agtcctttat acgaaaaggc gccgaagaag aaatttaaga aatgccaatt
      301 tttagagaag ctttcaggca aaaatatcgc taattgatgg gactctggag agacttaagt
      361 taagatacta taagtcaccg aagaagcaaa ataatattaa attattaaat ctttcaggta
      421 tacaaacagg gatatatagg ctaggattat tttccatgct gatatatctt ttttttatga
      481 aaaagtacag gaaataatat caaagttact taaatacagt aggtctaatt ttctagggag
      541 ggttatatat gaatgaattg aaaagagtat ctttgtacaa tatacacaaa gagcttggag
      601 caaaacttgt tgaatttgct ggttgggaaa tgcctttaga atatgagggt ataaataaag
      661 agcatgagaa ggtcagaaaa agtgcaggta tatttgatgt atctcatatg ggagaggtac
      721 agataaaggg agcagagtca gaaaaattta tacaaaacct agttacaaat gatataagcg
      781 cattaaagat aaatgatatt atatatacac ctatgtgtta tgaaaatggt ggagtagttg
      841 atgatttatt aatatataaa tttggtgaag aagattattt attagtaata aatgcaggta
      901 atatagataa agatgtagca tggataataa aacaaagtga aggatacaat gtagatataa
      961 aaaatatatc tagtgaagtg agtcaattag caattcaagg acctaaagca gaagaaattc
     1021 ttcaaaaaat cacagatata gacttaaact caataaaatt ttataagtca attccatcaa
     1081 caaaagtatg tggttgtcct tgtttagttt caagaacagg atatactggg gaagatgggt
     1141 ttgagatata ctgtaaaaat aaatatgtag aaataatttg gaatgaagtt ctaaaggttg
     1201 gaggagaaga tatatgtcca gctggtctag gttgtaggga tactttgaga tttgaagcag
     1261 ctctacctct atatggacat gaaataaatg aacacatatc tccaattgaa ggtggtttat
     1321 ctatctttgt gaaaacaaat aaagaatcat ttattggcaa atcaatttta tcaaaagaaa
     1381 aagaaagtgg agctaaaaga aagttagtag gatttgaaat gcaaggtaaa ggaatgccta
     1441 gaaatggata tgatataagg attggtgata aaacagttgg atttgtaaca acaggatgtg
     1501 catctccaac gacaggaaaa attttaggta tgggaattat agattctgaa tatgcaaaag
     1561 taggaaatga aataggtata gcaatcagaa aaaaagttgt tcctgctgtg atagttaaga
     1621 aaccttttta taaaaaacaa tataaaaaag ataatattat attaaacaaa gaaaataaat
     1681 tttcatatat acctgctaca agtgaagata aatcaaaaat gttaaaagta gttggattaa
     1741 attcggttga tgaattattt tcagatatac cagaagaagt aaaattaaaa agagatttaa
     1801 atttggaaat tggaaaatct gaattagagg taagcaaaat agtaaaaaga ttgtcagaag
     1861 aaaatttaag tttagaagac ttgacttgtt ttcttggtgc tggtgcatat gaccattata
     1921 taccatcaat tataaaacat ataacttcaa gatctgaatt ttatacagca tatactccat
     1981 atcaagctga aataagtcag ggaactttac aagtagtttt tgaatttcaa tctatgattg
     2041 cagaaattac aggtatggaa attgcaaatg catcaatgta tgatggagca actgctgcaa
     2101 tagaagcatg tataatggca atgaatcaga ctagaaaaag taaaatagta gtgtcaaaaa
     2161 ctattcatcc tgaaactttg tcaatattaa gaacttactt acaatataaa gattgtgaga
     2221 tagtagaaat tgacttttgt aatgaatatg gcactacaga tatagaaaaa ttaaaagctt
     2281 ctgtagacaa agatacagca tgtgttctta tacaaactcc taatttcttt ggaattattg
     2341 aagaaatgga agaaatagaa aagataactc atgaaaataa ggctatgtta attatgagtg
     2401 tagacccaat atcattagga gttttaaaaa caccaggtga gataggtgca gatattgttg
     2461 ttggagaagc tcaatcactt ggaaaccctc ttaattttgg aggtccttat gtaggatttt
     2521 tagctagcaa atctaaatat actagaaaaa tgccaggaag aatagttggg caaagtttag
     2581 atgtagaagg taaaattgca tatgtattaa ctttacaaac tagagaacag catgtaagaa
     2641 gagaaaaagc aacttctaat atatgttcaa atcaagcttt aaatgctctt gtagcttcaa
     2701 tatatatagc tactatgggt aaagaaggat ttaaagaggt tggtatgcag tctatgaaaa
     2761 aagcacatta tacttacaat aagcttgtac aaactggaaa atataagcca atatttaaag
     2821 gtaagttttt caaagaattt gctgtacaag gaaatcttaa tatagaaact ataaatgata
     2881 aactcttaga agaaaatata ttaggtggtt ataatttaga atacaattat ccagaattaa
     2941 aaaattcaac tttgctttgt gtgactgaga aaagaagcaa agaggaaata gataagttag
     3001 ttggaataat ggagggatta taatgaaaga atacaatagt ttgttgatag atatatctaa
     3061 aaaaggtagg aaagcatact cacttccaaa gttagatata tatgatataa aaatagaaaa
     3121 tatgattgac caagatatgg caagacaaag tgagttgaat ttgccagaag ttggtgaatt
     3181 agaattagtt agacattata cattattatc aaataaaaac tttggagttg atacaggttt
     3241 ttatcctttg ggttcttgta ctatgaaata taatcctaaa ataaatgaag atatggcagc
     3301 acttccaaac tttactggaa tgcatccata ccaatcttcg gatacagctc aaggttctct
     3361 ttctttgatg tatgatttat caagaagact tgcagaaatt acaggtatgg atgaagttac
     3421 actacaacct tcagcaggtt ctcatggtga gtttacaggg cttatgatta taaaagcata
     3481 tcatgaaaat agaggtgacc ataagagaac caaagtgata attccagatt ctgcacatgg
     3541 aacaaatcca gcaagtgcag ctatggcaaa ctttgatgtg attcaaatag catctgataa
     3601 aaatggagct gtagacataa atgcattaaa agaggttttg aatgatgaag tagcagctct
     3661 tatgcttact aatccaagta ctcttggttt atttgaaaaa aatataaaag aaatagctac
     3721 tcttgtacat gaagcaggag gacttttgta ctatgatggt gcaaacatga acgctattat
     3781 ggggataaca agacctggag atatggggtt tgatgtggtt catcttaatc ttcataagac
     3841 tttttcaact cctcatggag gtggaggtcc aggagctggt cctgttggag ttaaaaaaga
     3901 attagtacct tttttaccaa taccagtaat tgaaagaaaa gaagataagt atgtactaaa
     3961 ttatgataga gaaaaatcta ttgggaaaat taaaaatttc tatggtaatt ttggagttct
     4021 tgtaagagct tacacatata tactaactat gggaagggat ggacttaaag aggctagtga
     4081 aatggcagtt ttaaatgcta attatataaa ggaaagtata aaagatgatt atattttgcc
     4141 aatagatacc ttgtgtaagc atgagtttgt attaggtgga ttgccaagag gagaggccaa
     4201 tataaaaact atcgatattg caaaaagact actagattat ggataccatc ctccaacaat
     4261 gtactttcct cttattataa atgaggcact tatgatagaa cctacagaaa gtgagagtat
     4321 agagacatta gatagtttta tagaagctat gaaaagtgtt gctaaagagg caaaggaaga
     4381 accagaacta ttgaaaactg ctccacataa tactttggtc aaaagagtag atgatgcaag
     4441 ggcagtaaag aaacctatac taacatggtc acaaagataa atagttaaaa agtagagatt
     4501 taaagtataa agagaattaa atttgtaaga aaaagctatc taacttaatc tagtttagat
     4561 agcttttttg tatattagta tgtatggtat tttctatata aagttttaaa ttatattagt
     4621 gcttatatag aatattgata tgatgattat gtatagatat taataagaat tgttgaattt
     4681 atcttgcctt aactttattt actcttttat attaagaaaa acttaactaa aatatagcga
     4741 aattcataaa aatttaggaa atcttaagaa ttataatatt taaatcttta gatttatatt
     4801 ttattatata aactatagaa taataaaata aaaaaattga gaattttata atatatttgt
     4861 agatttgagt ataaaaaagt gctaagttga aaggagtaaa atatggataa ggttaattta
     4921 gaaaggtata gttgtgatat aaatgttggt ttaaataaaa atcaacttca aagtagaata
     4981 gatgataatt taattaatgc atttgataat ggaaaaacca aaacatataa acaaattttt
     5041 aaagacaata tatttacact ttttaatctc ataaatattg ttttagtctg tttattagtt
     5101 tcagttggtt cttttaaaaa taccttattt atatttattg ctgtaattaa tactattata
     5161 ggagtaatac aagagataag agctaaaaga ttattggaca atctatctgt tatagtttca
     5221 aataaggtat ctgtcataag agaaaaagag aaaaaagaga ttgatgtaca agaattagta
     5281 ttagatgata ttatgatttt aaaaacaggt aatcaaattt gtgcagattc aaaagtttta
     5341 aatggaaaat tagaggtaaa tgagtctttg gttacaggtg aatctgatat tattataaaa
     5401 aataaaggcg attttttata ttctggaagt tttgtagttt ctggagaagc ttttgtaaga
     5461 gttgaaaata taggaaaaga taactatgca aataagatag ctaatgatgc aaagatttct
     5521 aaaaaacata attctcaact tagaaattca ttaaatgcaa tattaaaaat tgtaggtata
     5581 ataataattc ctttaggaat tatattgttt gcaaaacaac attttggaaa tggagattct
     5641 atagctacat ctgtagtagg tacagtggca gctgtaaatg gtatgatacc agagggatta
     5701 acattactta cgagcattgc tctagctgta ggaactataa aacttgctag tcataaaact
     5761 ttagtgcaag aactgtactg tgtagagact cttgctagag ttgatacact atgtcttgat
     5821 aagacaggta caattacaga aggtaaaatg caagttgatg atatagtaat gttggaagaa
     5881 gtggatatta atgaaataat ggggaatata tgtaattctc tcaaagatga taatgcaaca
     5941 ctaaatgcaa taagagataa gtataatgtt ttagatacgt ataaagctaa gaatttaata
     6001 ccattttctt cagaaagaaa atacagtggg gttacctttg aaaataaagg tacatattta
     6061 ttaggtgcat ttgaatttat atttaaggaa aaaaataaaa aacttaggat agaagttgaa
     6121 agatattcta aaaaaggaaa cagagtaata gttttagctc atagtgattc ttatatggaa
     6181 gataataata ttcctgaaga tataaagcca ctagctttta tattgatatt ggataaaatt
     6241 agagatgaag ctaaggaaac tttagaattt ttttacaatg aaggtgtaga tataaaaatt
     6301 atatctggtg acaatcctat aacagtaagt gaggtagcta gaaaagcagg tcttaaaaca
     6361 gctagtaatt ttattgatgc aacaacactt aaaaatgata gtgatatata caaagcagtt
     6421 gataaataca gtgtttttgg aagggtaagc ccacaacaga aaaaacaaat gattttagca
     6481 ttaaaaaaac aaaatcatac agtagctatg actggagatg gtgtaaatga tgtgctagca
     6541 cttaaagaag cggattgtag cattgccatg gcatctggaa gtgatgttac taagaatgtg
     6601 tctaatttag tcttattaga ttcaaacttt gcatcaatgc caaaggttgt tatggaggga
     6661 agaaaagtaa ttaataacat tcaaagagca tcttctttgt ttttagtaaa gacaattttt
     6721 tcattcttct tgtcgttatt aacattattc ttcttcagta gacagtatcc atttgttcct
     6781 atacaattat cactcattgg ggctgtaaca gtgggtatac catcattctt cttagctctt
     6841 gaagcaaaca aaagtagaat ttcaggaaat tttttactca atatattaaa aaatgcttta
     6901 ccaggagcac tatgtgtgac agtaaatgta attattgttt acaatatagt tgaatattta
     6961 ggttatagtt caagtgtatt ttccacaatg tgtgctatat taactggtgt atgtggtctt
     7021 ttaatattaa gacaacaatg tttgccattt aataaagaaa gattattttt aatagtaagt
     7081 atgactattg catttgttat tgggattcta tttttaggtg gattattctc atttgtagca
     7141 ttgagtagag aattaattct cataactatg gcattagcaa tattaatgcc aattatgata
     7201 aaagttatgt tatttgtctt agatgagata ttagagacaa ttgttccaga tttaaattaa
     7261 ttttaaaatg atataaataa gagcagtttt agttgaactg ctctttttag atgtgtttaa
     7321 ttttatgtaa gaatatgtat ttttattaat cagctatata tgagcaacag tagtcaacaa
     7381 cttcattaac ctttaagtca aaacttgaat cactaggaac attaaatttt tgcccttctt
     7441 tataagttac aaactcattg cttccaggca gttttacatc catacttcca gcaagcatct
     7501 ccattatttc ttcttctcta gtagaaaaag tgtattctcc tggcaacatt aatcctaaag
     7561 tttttctttc tccattaggc agtattatta ctctgctact taccttacca tcaaaataaa
     7621 cgtttgcctt ctttactgca gtaatattct taaattcact cattagataa aacctccaaa
     7681 attatttgta catatatatt atataaaaat aatgtaaaaa atgctatata tttacaaaaa
     7741 aatttagtat ttattaaaaa aattaataag aataaaaaag agatgaaatt gcttaaattg
     7801 ataaaaaata taaaataaat tgcaaagtaa gcaagaaaag aataagttga agagttatat
     7861 attataaata ttaaaaattt tagtattaat aaaaaagata ttacattaat atgaaaatat
     7921 aggagattag aagtgtttga taattacagt acatgttaga tatatcattg taaattaaca
     7981 tagctttgaa tataataatg ttcagaaggc aatatttttg aggtctgaat ccaataataa
     8041 tatagtctta tgattgtaga tatcaaaaaa tgtaagttac taggaatacg ttttgtagat
     8101 ttatatatga ttaataacta aagctataaa taaacaaaat aaaaagagta tgtttcaacc
     8161 tatagctttt agatttaaaa tatactcttt tattaattat agttgtataa attaaccata
     8221 atatataatt aaaattttta ttataattat atattaggtt cttcaattag ttttccacat
     8281 acatatctat gaatgatatt tctatcatca ccaacatata taaatctttc aagtctttca
     8341 agtatagaat aattgtcatg atttaaattt gaatcatcaa taatcaatgc atcaaaatca
     8401 taaccttctt caaagctacc aacttttcca aagaaacttc caccactttt ggttgcaata
     8461 taaaaagcct cagataaaga taagaaatta tacttcttac cagagttaac ccaatataat
     8521 ttagataatt gtattgcaga taccattgct ttaaatattg acaatgtatg tccaccactt
     8581 atatctgatc caagtgctaa tctaatatta ttatcgatta attttctgat aggcatagca
     8641 ccactaccaa cattaaagtt agaagctgga cagtgaactg ccataacatt attttctctc
     8701 attaaatcta tttcatcctg agaacaatga acacaatgag ccattaaagt aggagtttga
     8761 ccaaataaat tatatctatt atatgcatct ccatagaatt tagattcagg ttctagactt
     8821 ctaacccatt caatctcatc ataattttca gataagtgtg attgaatagg aatattatat
     8881 tttaaacata aatctcctaa accttttaaa agttcacttg tacagcttgg tataaatcta
     8941 ggtgttatta ttggttttac aatagaatct gtatcactat acttatttat aatttcttct
     9001 gtatcagtaa tagaatcctg agtgttttct aataaagtat cagggcaatt catattcata
     9061 tttactttac caacgtatgc tccaagacca acttctttga atatatccat aagtttcata
     9121 gaggcttctt tatgtactgt tgcaaacact gctattcttg tagttccaga tttccaaagt
     9181 tcttttacaa attttttata catttttgtt gcatattcta tatctttata atttgattct
     9241 tctggaaatg tataagtttc aagccatggc aaaagctctt tatccatagc cattccaaga
     9301 tttttaaatt gagaggcatg ggcatggagg tcattcatac caggtattat aagcttatca
     9361 gtgtaatcta ctacttttag gtttttgtat tccttaggta tctctttaaa tattcccttt
     9421 actttacctt tttccagcat aatataacta ttttcaaata ctgtgaaagt ttctgaagtt
     9481 tttgtaaaaa ttatatttcc ttttagtact tttaacatat aggtctcctt agttttaaat
     9541 ttgttttaat atatgtaggt aaaattttgt tttacaaatc aaaattttac cacatgtatt
     9601 atatcaaatc taaactataa atactattag ataatagcaa atattagtga aatacagttg
     9661 tttactatta ttatatataa ctgacattta agtgacattt aagaaaaata taatgcctac
     9721 ttacataaaa tggaatgtta tttaaagaga actttgatta tattttcaga agttttttta
     9781 tccatatcgt ttaaaacaag aaaatatcta ttcatagtta tttttatatt agtatgtcct
     9841 aatctttcag agatgatttt tatattagtt ctagctagaa gaagaactat tagaatagat
     9901 aatatagtaa gtatttacaa atatgtaggt gttcttaaat tgataaatta ttccatttta
     9961 attttatagt ttgaatttta tgatataata aaaatatata aattttgcag tgagcgatat
    10021 ttttgataaa gtagggttta acagttgcaa tgtaagggat tgagggtgta tgataaatgt
    10081 tatcaattgc actactcatg gttcactgca aatttgagag agttgtatgt gtgtaagtac
    10141 tgaaaatact tagtttattt tggggtttta tattaactaa gtggtatgta aataaacctg
    10201 gattagcttt tatccaacat tcttctctgt tttatattaa ctaagtggta tgtaaatgct
    10261 actagaccag ctaccactaa cagtacgaat gagttttata ttaactaagt ggtatgtaaa
    10321 tatagtataa acactttcaa ccgtccttga aagtgttgtt ttatattaac taagtggtat
    10381 gtaaatttac ttagaaacaa atatattgtt attaacatac caagttttat attaactaag
    10441 tggtatgtaa atttaagagc atcttctata cctattttaa agtttactgg gttttatatt
    10501 aactaagtgg tatgtaaatt tgctatctga gtattaattt cttctatttc ttgcctgttt
    10561 tatattaact aagtggtatg taaatattat aatatgtaac tatatctaac ttttatatta
    10621 tcgttttata ttaactaagt ggtatgtaaa tgtatgtctt ttaaagttgc taatacaact
    10681 gttgggtcgt tttatattaa ctaagtggta tgtaaattga atatcactac accaaatttt
    10741 tagacattca aacgttttat attaactaag tggtatgtaa attaattagt ggtgttataa
    10801 gactcatttt ttgattttag ttttatatta actaagtggt atgtaaattt gacttactat
    10861 gtattacttc taagttactt atatcgtttt atattaacta agtggtatgt aaatcaagat
    10921 atttttaata aaatttctga gcgaactgtt ttgttttata ttaactaagt ggtatgtaaa
    10981 taaaaataga acaaattatg gaaaaatact agtaaatgtt ttatattaac taagtggtat
    11041 gtaaattctc atcacaacac tatatacata gtaaattaac atagttttat attaactaag
    11101 tggtatgtaa atgcatgtta aagtggctaa ttggaactat gattacagtt ttatattaac
    11161 taagtggtat gtaaatagtc cagaccctaa tcctcctgtt gacccagagt tttatattaa
    11221 ctaagtggta tgtaaatata gtcaaaagca acatcataat cacgtatatt atcgttttat
    11281 attaactaag tggtatgtaa atcaacatgt cagtaatatt gaaacaggaa attcgagtgt
    11341 tttatattaa ctaagtggta tgtaaataac actttaaaaa ctaatgtatt cagtataaga
    11401 gattgtttta tattaactat gaggtatgta aatcttgtta atgcaaataa acaacatagc
    11461 actattaata gttttatatt aactatgtgg tatgtaaatt tgcaacaagt ataggtaaaa
    11521 taccccaata atttatacag cattttctcc tttaaaatat aattattttt
//
LOCUS       DAGSWI010000129        11532 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.130, whole genome shotgun sequence.
ACCESSION   DAGSWI010000129 DAGSWI010000000
VERSION     DAGSWI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.130"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            435..1013
                     /locus_tag="MJF80_003111"
     CDS             435..1013
                     /locus_tag="MJF80_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBZ0281751.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            1052..1630
                     /locus_tag="MJF80_003112"
     CDS             1052..1630
                     /locus_tag="MJF80_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBZ0281752.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1716..2345
                     /locus_tag="MJF80_003113"
     CDS             1716..2345
                     /locus_tag="MJF80_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBZ0281753.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2335..5094
                     /locus_tag="MJF80_003114"
     CDS             2335..5094
                     /locus_tag="MJF80_003114"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBZ0281754.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            5181..6743
                     /locus_tag="MJF80_003115"
     CDS             5181..6743
                     /locus_tag="MJF80_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBZ0281755.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6744..7880
                     /locus_tag="MJF80_003116"
     CDS             6744..7880
                     /locus_tag="MJF80_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBZ0281756.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7944..9020
                     /locus_tag="MJF80_003117"
     CDS             7944..9020
                     /locus_tag="MJF80_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBZ0281757.1"
                     /translation="MKYFDYICKDDLEKIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNRHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8998..10236
                     /locus_tag="MJF80_003118"
     CDS             8998..10236
                     /locus_tag="MJF80_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBZ0281758.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10290..11378
                     /locus_tag="MJF80_003119"
     CDS             10290..11378
                     /locus_tag="MJF80_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBZ0281759.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLISKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
BASE COUNT         4634 a         1158 c         2127 g         3613 t
ORIGIN      
        1 tatatgaaga actcttattt ttataggtag tatcagatat aaattcaatt agaggttcag
       61 tagacttttt tattgaaatc tttaatggtt taatagtaca aatcatattt tttctcctat
      121 aataagatga tataatctat tataaaacaa cttaaaacaa tatagtacaa taattttgta
      181 gtttcaaaat aaaatatgta cttgtttata taaatgatat ataattacat attaaacata
      241 gcttttatgc atttaataaa gtatgtaaaa tctatgtaaa aagtaagaaa tataaacatt
      301 tttttaatta tattttaaaa atatcaagca agttttcttc acaaatgaat aaaaaatgtt
      361 ataatatgat taaagaaaaa gataaataat tagcaagatt tgtttagcta aaattaacaa
      421 gggagcgata aattatgggt ataacattat caaaaggcca aaaggttgat ttaactaaag
      481 gaaatccagg ccttaaaaat attttagtag gtttaggatg ggatactaac aagtatgatg
      541 gtggatttga ctttgattta gatacagcag catttttaac aggagcaagt ggaagtgtta
      601 ctaatgatgg agattttgta ttttacaaca atttaaaaca tacatctgga gcagttgagc
      661 atctaggaga taacagaact ggcgaaggtg atggagatga tgaacaaata gttgttgatt
      721 tatctaagat tcctggagaa ataagcaaga tttcttttac tgttacaatt catgatgcaa
      781 cagaaagaag acaaaatttt ggacaagtaa gtaattctta tataagaata gtagataaag
      841 acacaaatga agagttaata aagtatgaat taggagaaga ttttagtata gaaacagcta
      901 tagtagtagc tgaaatatat aagcacaatg gagaatggaa gtttaatgca ctaggttctg
      961 gctttgaagg tggtcttgct gcattatgtg gaaactttgg aataaactta taatttcaac
     1021 tataacaata aactaaaggg ggatattttc catggctata gtattaaaaa aaggacaaaa
     1081 aatcgattta actaagggaa atccaggtct taaaaatata aaacttggac taggatggga
     1141 tacaaattca tttgatagtg gctatgatta tgatttagat gtaagtgttt tcatggttgg
     1201 agaatctcaa agagttgaaa aagatgaaga ttttatattc tacaataact taaaacatcc
     1261 atctggagca gttgaacact taggtgataa tagaactggt gaaggtgatg gagatgatga
     1321 ggaaatatta gtgaatttag agacaatgcc aaagcatata caaaagatag ctgtaacagt
     1381 tacaatatat gaggctgctg aaagaagaca aaattttgga caagtaagta actcatatat
     1441 aagagtatta aattctgaaa atgatgaaga aatattaaga tatgacttag gtgaagaatt
     1501 tagtatagaa actgcaatta cagtatgtga gatatataag tataatggag aatggaagtt
     1561 tagtgcagtt ggagcaggat ttgaaggtgg cttagaagca ctttgtagaa actatggctt
     1621 aaatgtttaa tttaagcaat ataatatatt aaatataaat aacctataca aataatgtat
     1681 aggttatatt atataaaatt ttgtggaggt ttagtatgtc gattaattta tcaaaaggtg
     1741 ataaaataga tttaaaaaaa tctaacccag gactgagtaa catacttgta ggtctaggat
     1801 gggaccctgt gcaacaatct ggcggaggtt tttttaaatc gttatttggt ggaggacaag
     1861 ctgatataga ttgtgatgca tctgtattta tgcttaatca agaaggtaaa ttaagtggaa
     1921 taaaagattt aatttatttt ggaaatctaa aaagtgcctg taaatcagtt ttgcatacag
     1981 gagacaatct aactggtgag ggtacaggtg atgatgaaca aatcctagtt aatttggata
     2041 aagttccatc aaatattcat aaattattgt ttgtagtaaa tatatacaat tgtgtagata
     2101 gaaaacaaca ctttggaatg atagaaaatg cctatataag agttgaagac caaggtaata
     2161 agaaagagat agcaaaatac aatctatctg ataattattc tgaaaaaact actcttatag
     2221 taggagctat atatagaaaa gatggaagtt ggcaatttaa agcaattggt gaaggaacaa
     2281 aagatgcggg tcttaaagaa gtaatgcaaa atttagacag gattgagtgt gcatatggaa
     2341 tttaataatg atatcaaata ttataatgta gataaagaag aagttacaaa agacttatct
     2401 gttaatcctg aaaaaggttt atcagaatca gaagtcaaga ctagaagaga aaaatatggt
     2461 ttgaatgaat ttactccaaa agaagagggt agtttttggg atgatttaaa ggaaagttta
     2521 agtgaaccaa tgatagttat cttgattatt gcagcagtag taagtgcagt aattggggaa
     2581 actcatgatg caatagggat tgtaggtgct atagcaatag gtatagcaat aggtatgatt
     2641 acagaaggtc gttctaagaa agcagcagag gctctttcta agttgactga aaacatagaa
     2701 gtaaaagtat tgagagatgg ggaagtacat caaatttcta agagtgaatt ggtaccaggt
     2761 gatatagtat atattgaaac tggtgacatg gtacctgctg atggtagact tatagaaagt
     2821 ataaacctta aaattagaga agatatgtta acaggagaat ctgatgacgt atctaaaaag
     2881 tgtgatgtag tagtttctat ggaagagata gaatctaaag gagcagtagt agtacaggag
     2941 ccaatacctg caaaacagat aaatatggtg tttggtggca cacttgtagc ttatggtaga
     3001 ggagctctag ttgtaacttc aattggagat aatagtgaaa tgggtaaaat tgctcaaaac
     3061 ttatcagaaa cagatgagca aactccactt cagataaaac ttggaaattt aggtggtttg
     3121 atagccaaag tttctagtgc agtagcagga ttactattta tatttatggt tttccaaatg
     3181 ataagtaaga atgtattaaa tgtagatatg tctggaatat taccgttttt agaatcaata
     3241 gacccagtta aaacagcatt tacagtatgt gttgcgttga tagttgcagc agtgccagaa
     3301 ggtcttccaa ctatgataaa tatgacactt gcaataacaa tgcaaaaaat ggctaaaata
     3361 aatgcattgg ttactaaaaa agaagcttgt gaaacgatag gttctgtttc tgtaatatgt
     3421 tcagataaaa ctggtacatt aactgaaaat agaatgactg tagaagttgc atatgttgat
     3481 ggcagatata ttgaaagttc agaagaggaa ataaatagtt attttgaaga aaactgtatg
     3541 ataaactcta cagctgatgt agagcacaat gatggtgata ttaagtattt aggaagtgct
     3601 actgaatgtg cattgctttt atattacaaa aacgtagatt atagacaagt tagaaaaaat
     3661 tctaatatag tagctcaaaa tccttttact tcagattcaa agagaatgac ttctgttata
     3721 ggtcaagata atcatcatgt actattatca aaaggagcac cagaagtctt acttgaatta
     3781 tgttcacata ttcaaagagg aaaagacata gttccattaa ctgaaaatat aaaacatgag
     3841 attttagaag aaataaagaa attgcaaata aaatcaatga gaactttagg gtttgcatat
     3901 aaagaaatct cacaagctga agaagaagca gcagtaactg ctgaaagtga tgctatgaat
     3961 gtaagtgcaa tggaaaacaa tttagtattt agtggttttg ttggtataag agacccactt
     4021 agaaaagatg ttatagagtc tgtaaataca gcaaataaag ctggtgtttc tgtaaaaatg
     4081 cttacaggag ataatataaa tacggcaaga gctataggtg aagagttagg cctattaaaa
     4141 aataatatga gagtggttga agcttcatat atagatactt tagatgatga agaattaaaa
     4201 caagagatac aaagtatttc aatcgtggca agaagtaaac cagatagtaa aatgagaata
     4261 gtttcagctc tgcaaaagag cggagatgtt gttgcagtaa caggtgatgg tataaatgat
     4321 gctccagcac tttcaaaagc agatgttggt atagcaatgg gtatatctgg tacagaggta
     4381 tctaaaaacg cagcagacat aattttaact gatgatagtt ttagtaccat tgtaaaaggt
     4441 attaaatggg gtagaggaat ttatgataac ttccaaagat tcgttcaatt ccaattaact
     4501 gtaaatgtaa ttgcattttt agtggctata atatctcaag ttatgggaca agagatgcca
     4561 tttactacaa tacaattatt atgggtaaac atcattatgg atggaccacc agcactagca
     4621 ttaggtttag agccagttag ggaccatgtg ttaaatagaa aacctgttga tagaaaagcc
     4681 aatataattt ctaagtcaat ggtgtataca atagtattaa atgcatttta tataacagca
     4741 atactaatgt tacaatctac atttaatttc ttaggagttg atgaagcgca taagggaaca
     4801 gtaatgtttg cgttatttgc ttttagtgca ctgtttaatg catttaactg tagagagttt
     4861 aatgcagata gtatcttccc taattttaca cataacaaat tagcactaca aataatagtg
     4921 ttgacaggaa ttgcacaagt aatatttact cagctattcc aagatttctt taactctgta
     4981 gcaatggatt ttatgacatg ggtaaaagtt ttatgtgtag cagcaagtgt aattgtggtt
     5041 aatgaagttg taaaatgtat aataagactg gtaaaaccaa aaaagactgc ttaaaacaaa
     5101 ttttttagaa gaaaacagac tgactcaaaa tatgaatttt gggtcagttc tgaataaaca
     5161 tttaaattgg gaggatagtt atgggtgact caaaggtatt tgaaaaaata ttctccagcc
     5221 aatcagatag aggaaattac actccttcaa agggatatct aagttatttt atttcgtata
     5281 ttggtttaga agatgaagtt ttgtacaatc tcgagatatt taagacaaaa caaaatattg
     5341 atagcaaaaa agatattgct ttatttactg atgttatagc aaatcctagt gattttgata
     5401 ttataaatta ttttaaaagt ggacttcaaa aatatagaac tagcatggaa gatgtagata
     5461 taaatatttt gggatttgaa gagattgatt ataaaataaa gcaagcaatg gatagagtgt
     5521 taaaagaaga ggaaaaagaa tttactaatg atagagttaa acaaaatttt atagtgaaaa
     5581 taatggcatg gataaaaatt tatatcggtg ctttagatat aaataaaaat gaagctccaa
     5641 aagtaatatt ttatggagat ataaaaaaac atgaagttta tttactctta atattatact
     5701 tggcaggttt tgatgtttta tatcttaatc caaattcaaa gagtaatata gatattttaa
     5761 aatcagaaag atacaatata gagtttgaag aagccaacat aatagaagaa aaaatttctt
     5821 ttgaagaaag ggttatttta ggggaaaaaa tagataaaag ttctgtaaaa aaagctttta
     5881 ctgttggggc agaagcatca aagcgtataa gtgaggaatt gttaaatgat gcaggattta
     5941 taaaaccttg gcaactccaa gatagaaaaa taaaaaatct acttctaagt tcaactgttg
     6001 atgaaatatc aatatactgg aatcaaccac ttaaattaag accaggattt aagtttaatg
     6061 atgctattgt agaagctcca aattttcttt ctaagattaa tggaatatat aacgataaga
     6121 atgaatatat taagttttta gatttattaa gagattcgga aagttcaaca tttattgaat
     6181 ttaatggaga tgtagataga ttttcaaaag cttttacaag agaagctttt agtttatcct
     6241 ttttattgga ttcaaaaggt gcaatagata aaaactctgt tttaaataat aaagattatt
     6301 ccatatcaac actagcattg aatcaacaaa ttatgatttt agagaaagtg gaagagttgt
     6361 tagaaggaag tatgtttttg aatggtttaa gtggagaaga caagataaaa ggtctattta
     6421 cagtacttca catggataag aaatttgttc atatgatgaa caatttcgat tactcactta
     6481 taaacccaaa attgattata tacatgtaca agagtattgt atttgataaa gaaatagttt
     6541 ttcttatgct tttacttagt aagattggat ttgatattat catattatgt ccaggaggag
     6601 aaaacaatat agagaatgtt ataaataacc aattgattga tgtacacaga ttggataaaa
     6661 tggtatatga ccttaaatta aattcgcttg aaaatgatat acctttactt aaaaaaattt
     6721 ttggaaagag gagacgattt tagatgggga taaatataaa tagtttttca aaagataaga
     6781 actctgtaga ggttactgag aatttgccta cagcagttca agaagaaaac ttcgatataa
     6841 tggaatatac aaataataaa aaaaatgaac tgagaaagtc taaggaagtt gaggcattga
     6901 catcccttat agaagtagaa aatccagata cgattttaca gtttggtagg aaagcatctg
     6961 aaggagtagc aagagtttct gatagccttt taaatacaat aaaattaaat agaaatgaag
     7021 aaaattctaa aatgctggtt catttaacta aaataatgga caagtttgat ttagacgatt
     7081 ttcaagaaac taaggaacca aatttcgttc aaaaactttt caaaaaagct aataatgcca
     7141 ttgaaatgat gtttcaaaag tatgaaacat tgggaggaga agttgagaaa attcaaattg
     7201 aacttgaaca atacgaaaga gatatagcac tttctaataa gcaaataggt gctatgttaa
     7261 atgaaaactt tgagttttac aatgaacttc aaaaatacat tgttgctgga gaaatggcaa
     7321 tagaagaaat ggataatgaa atactacctt tctttaaaca aaaatcagag actagtggag
     7381 accaaatgga tgttgtcaat tatcaagaac tattaaaagt ttatgatatg ttaaatcaaa
     7441 gagtctatga tttaaggata gcagaaaata tagctatcca aacaatacct atgcttagag
     7501 gtatgcaaca taataattat ggtttaatta gaaaaataaa tagtgcattt gtcgtaactt
     7561 taccagtatt taaacaatgt ttatctcaag ctatattact taaaaagcaa gaattacaag
     7621 caaaatctct aaaagcactt gatgacaaaa ctaatgaatt attgcttaga aatgcacaaa
     7681 atgtaagtac tcaaagtgca caaatagcta gaatggctgg aacaagttct gttcaaatag
     7741 aaacattgga aaaaatgtac aatactatca aatctggtat agatgaaact atgagaatag
     7801 aagaaaacaa tagagcttta attaaggata atacaaagag attggaagaa ttaaatacga
     7861 ctataattta taataagtag tattatatta agggctaatt tgtataaagt tggccctttc
     7921 tatgtatatg aggaggctag acattgaaat atttcgatta tatctgcaaa gatgatttgg
     7981 agaaaatttt tttgaaggag ccagaagatt ttagtgctaa gactgaaaaa gatgtgttga
     8041 aatatgcact gggcgctttt ttatacgttc cagctactca atataatatg atttataaga
     8101 gtattatagg ggatgttaag ggagttagac cattagctat ttgtttagaa gatgctgttg
     8161 gagtaaatgg agagttggag gctatagaaa atcttagatt aattttaaaa aatattagca
     8221 atgaaagtat aactaataaa gatggaatac cactaatttt tgtaagaata aaagatgtgg
     8281 agcaactgtt gagaattaaa gagattataa ttaaaaatag acattctata acaggaatat
     8341 taattcctaa agcaaattcg gagttaatag aaaattgtat agaagcttta gattctatga
     8401 atcttcaaga tatgtatgtt attccgataa tagagacaaa agaatttata tataatgaaa
     8461 aaaaagaact tagttttact aatttgtaca atgctatttt aagacataag tcaagaatat
     8521 taagcattag agtaggcata actgatatat tagggatgta tggtataagg agagataaga
     8581 acttctctat atataataat ttgatttgtt cttcatttat attagatata atcacatatc
     8641 ttcaaagacc agagttagat atacctataa gtggtggagt atcagaattt tttgatatga
     8701 cgaataagga tataagaaat aagtatatag aagaaatatt attggataaa tatcatggat
     8761 taattggaaa gaccgtaatt catccaatgc aaattcaaat tgtacaagcg ctatctacag
     8821 tatcatatga agattttact gatgctttgg atatactgga tagtacagac agtaagtatg
     8881 gtgtgagcaa gggtgtatta ggagagagaa tgaatgaaac aaatccacat tttttatggg
     8941 ctaaaaaaac attgattcta tctaaaatat atggtgtatt aaataaagga gtagactatg
     9001 aagaattgct taaattttaa ttgtgagatt gacattattg ataatccatt gaatttagaa
     9061 ctaaatgatt ttttagacgt aggaataaga aataatagca aaagacgttt tttgtttatc
     9121 tcaaagaaac tcggtaaaca tttagcatgt aagccaagtg agatggataa tttgggaaaa
     9181 ttaatggcta caatttataa tgaaaaaaat aaggaagaat caaatggtac tgttatagct
     9241 tttgctgaaa ctggtacagc tgttgggcat agtttttttg attacttatg tggagattat
     9301 gaatttattc atactactag agagcagttt gacgagttag aaaatctgga atttttagag
     9361 gaacattccc atgcaacaga ccagaattta tactttggta tgcttaacaa ctttaaattt
     9421 ggagatgaga taatattggt tgatgatgag attaccacag ccaatacatg catgaatata
     9481 attaggaaaa ttcaaagcat ttatcctaaa aagaaataca ctatatgctc aatactcaat
     9541 tgggtaagtg atgaaaattt aagtaaagtt actcgtttag aaagagagct agattgtaag
     9601 atagattttg tatacttatt taaaggaaac tttaaatttt atatagatga agatattatt
     9661 tcaaattaca acgataatta tagtattact aaattagaga ctatttcaag tgataaagta
     9721 aatttagtag taaatcatat ttatgttgat ttagaagatt atattggaaa taagaaatat
     9781 gtaaaatata caggaagatt tggaattaat agaagagaac aatttagatt aaaagaaatc
     9841 gttaagagag aaagtaaaaa aataactatg gccagtaaag aatctttaaa aaatgaaaaa
     9901 gaaaagattc tatttttagg tactgaagaa tttatgtata tacctatgct gtttgcaaaa
     9961 gaaaaagaaa atcaatatga tatatactat cattcaacaa caagaagtcc aattattaat
    10021 attgataaga ataattatcc tattaaaagt aaatttatgc taaagagttt ttataatata
    10081 gatattcaaa attatattta caatatagat aaacataatt tttctaagtg ttatttattt
    10141 gtagagctga ataaaagtga aaattcttat ctagaattta ttgatataat taaaaaaaca
    10201 agtataaaag aattaactat agtatgttgt agctaaagtg ttttaacagg ataaaatata
    10261 tcacgatttt aagataggag ttgagtcata tgagagattt tagcacgtat tctaaagatg
    10321 atgttatttt tttattaaaa gatatatcaa atttaattga agaagaagga aataaagaaa
    10381 gagaattaaa aatacaaaat ggtagacatt attcagaaat gataccaata gagtatgaag
    10441 ttagtgaaga ttatctgaat ttatatcatg agaaactaag agaaaataaa gataaacttg
    10501 cattttctat aggagttatg tgtgaaaaaa taattaaaaa aaatggaaat gatgtagtac
    10561 tagtttcact tgctagagca ggaacaccta ttggtatttt ggcaaaaaga tatataagaa
    10621 gcaagtataa tgtaagttta cctcactata caatctctat aataagagat aagggaatag
    10681 atatgaatgc aattgaatat atagtaaaga atcatccgag ctcaaaaatt caatttttag
    10741 atggatggac tggtaaagga actatatcaa aagaacttga aaaagcatgt gatgaactta
    10801 atgttaaatt taaaaaatat tttgactcaa cacttgctgt attagctgac cctgctggat
    10861 attcaggact ttatggaatt agagaagatt tcttaatacc aagtgcatgt ttaaattcaa
    10921 cagtttctgg tttagtaagt agaactgtac taagagagga tttaattagt aaaaatgact
    10981 ttcatggagc taagttttat agacatttaa aagataaaga tgaatctatg aactatatcg
    11041 aaactataga agaatgtttt aaaaatcaat ttaagaatat atctgatgaa gttgaaaact
    11101 gggaaagtga cattataaca agagatggat actttgatgt tttaaatata aaagaaaagt
    11161 ataacataac agatattaac tttatcaaac caggtgtagg ggaaacgact agggttttac
    11221 ttagaagagt tccgtataag attcttgtta aagacttaaa tgataaatct ttagaccata
    11281 tattcatatt agctaaggag aaaaatgttg aagttgaaca aatggattta aaagcctata
    11341 aatgttgtgg aataataaaa aatatgaaag atatttagga aatatattag acaggagaat
    11401 attgaatgat tgttttttca gatttagaca ggagtatcat ttactccaat aaatttttaa
    11461 atgctgatag taaatatgct aatatagaaa tttataggga aaaagaaata tcttatatat
    11521 ctttagatac ta
//
LOCUS       DAGSWI010000130        11359 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.131, whole genome shotgun sequence.
ACCESSION   DAGSWI010000130 DAGSWI010000000
VERSION     DAGSWI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.131"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            100..708
                     /locus_tag="MJF80_003120"
     CDS             100..708
                     /locus_tag="MJF80_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBZ0281760.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISCQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKCLA"
     gene            1035..3932
                     /locus_tag="MJF80_003121"
     CDS             1035..3932
                     /locus_tag="MJF80_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBZ0281761.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            4253..4465
                     /locus_tag="MJF80_003122"
     CDS             4253..4465
                     /locus_tag="MJF80_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBZ0281762.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            4478..4699
                     /locus_tag="MJF80_003123"
     CDS             4478..4699
                     /locus_tag="MJF80_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBZ0281763.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            4799..6955
                     /gene="feoB"
                     /locus_tag="MJF80_003124"
     CDS             4799..6955
                     /gene="feoB"
                     /locus_tag="MJF80_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBZ0281764.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            6981..7133
                     /locus_tag="MJF80_003125"
     CDS             6981..7133
                     /locus_tag="MJF80_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBZ0281765.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSECSSSKS
                     CHSAKR"
     gene            7313..8023
                     /locus_tag="MJF80_003126"
     CDS             7313..8023
                     /locus_tag="MJF80_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBZ0281766.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            8108..8878
                     /locus_tag="MJF80_003127"
     CDS             8108..8878
                     /locus_tag="MJF80_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281767.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            8871..9602
                     /locus_tag="MJF80_003128"
     CDS             8871..9602
                     /locus_tag="MJF80_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281768.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            9636..10595
                     /locus_tag="MJF80_003129"
     CDS             9636..10595
                     /locus_tag="MJF80_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBZ0281769.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(10693..11289)
                     /locus_tag="MJF80_003130"
     CDS             complement(10693..11289)
                     /locus_tag="MJF80_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBZ0281770.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         4366 a         1167 c         2033 g         3793 t
ORIGIN      
        1 aatgattaat ccaaaaataa atttgatttt agaaaagtaa attgacatgt aaagaagaat
       61 atattaaact aagtataaaa aatttgctgt tttgtaaagg tggtggataa tctgataaag
      121 tatatacata aaagtgatgt agctattgtt gttttgcatg aaatctatgg gattaatagc
      181 tttatagaag atgtttgtca aaagtattat aaatatggat atgatgtatt ttgtcctgaa
      241 cttctgggag aaaaaagagt ttttccatat tcaaaagtcc aagaggcata tgattttttt
      301 ataaataatg ttggttttga tatttataaa aaagttgaaa agcttataaa tgatttaaaa
      361 tcagagtata aatatgtatt tgttgctggg tatagcgtgg gagcgactat tgcatggaga
      421 tgcagtgaag attctttttg tgatggcata atttgttgtt atggctctcg gattcgagat
      481 tacatggatg taattccaaa atgtcctacc ttattagtat ttgcaaagca cgattcattt
      541 gatgttgatt ctatttcttg ccaactttct aggaaacaaa atatccaaat tgagatttta
      601 gatgcaaatc atggttttat agatagatat tcaaataatt attctgatat tcaaacaaaa
      661 gtatttacta ttaaagaaaa aagttttctg tctaaatgtt tagcatgatt tagattatta
      721 taaaatatat agaaagattt aattaatctg aaaagttttt atgacactat tgatatttaa
      781 aatattagta gtgttattat attttttgaa ataaaattaa tcactagaat tatagtgtta
      841 gttgtgcttt tatgacttat aagaaactaa tattagatag tgtatttaaa tagataataa
      901 taaatttatt atagttgaat aagaatatta tatgagaata ttgcaatttg atatatttac
      961 cattacaata aaagtagtta caactagaaa accaaataag ttaaagtaag ttggcaaatt
     1021 ggaggttttt aaatatgaaa gcatttaaaa agcacttctt gatactttct ttgatatttt
     1081 tacttattat atcatgtttt aacaatatac tatgctttgc tgatgagtct gaaaattcaa
     1141 aaaaaataag agttggatat tgtgatgact atggaataat atcctcatca aaagagcatt
     1201 cttatacagg atatggttat gattatctaa gagaaatctc aaaatataca agatgggaat
     1261 atgaatttgt aaaagggtct tgggaagaat gtttagacag actagaaaag ggtgaaatag
     1321 acttactcgg acctttacag aagaatgatg aaagaaataa actatttaat tttcctaaac
     1381 taagttcagg atatgaatat agtgcactat atacaaagga tagtaacaat aatatgtttt
     1441 atgaagatat aagttccttt aagggaatga gagtagctgt acttagagga aactttcata
     1501 atactgcatt tgagaagtat agagaagaaa ataatttttc agttgaatat atatactgta
     1561 attctattga tgaattaatt gagtccgtca atgaaaaaaa ggcagatgca tttgtttgtg
     1621 gaagtattat aaatgctaaa ggaatgaaaa tagttagcaa attttcagta gaaccttttt
     1681 attttgctac tgcaaaagat aaacctaatt tggtaaaaga attagattat gctttaaaag
     1741 aattaaaaat aaatgatatg tattatgagt tggaattata taaaaaatat ttcaaaagag
     1801 aggtcaacaa ttccatagca tttactcggc aagaaatgaa ttttattaaa gcaaacccaa
     1861 aacttactat ggtatatgat tcagagtggt ctcctgtaga gtattatgat aaagaaagca
     1921 attcatttaa aggtataagt tccgatttaa tgtcaattat atcaaaaaaa tgtggtataa
     1981 aatttgaata tattaaaaca aaaaattata atgagagtct tgattacata aaaagtggta
     2041 aggcaactat gctatgtggc tcaatcaatg aaaatgataa ggcaaaacga tttaatatga
     2101 aattgacaaa tccatacata aatattccaa tgattatggt aggaaaattg gataccaatc
     2161 tcaataatga tttaaatatt gctttaacta gtagttataa gtctattgat tcttatatag
     2221 aaaagagttt tgaaaatgct aagacaacat cttataagag tgtagaaagt tgtctaaatg
     2281 ctataaatga aggtaaagca aacttaatga tacttagttc ttatcagttt gatgaacttt
     2341 taagagaagg aaagagtgag aatttatctg ttatatctgt tttagataca agctatggga
     2401 tgcgtatagg ggtatcaaat aaaactgacc ctatattagt gtctatttta aataaatcaa
     2461 tagacaaaat tactgaagaa gaattaagtg attgtatata ttctaatact attgacaaac
     2521 cttataaagt accatttggt gttattttta aagaatattc aatacaaatt atttcttttg
     2581 tatgtatttt attcttaatt gctattaaat atataattta taataaaaag aagaaagagg
     2641 attatctaag gaagatagca tatacagacc cattgacagg agcagatagt atagataaat
     2701 ttaaaataaa ttctaataaa ctgtttgcta aaaataatcc agaagaatat gcgttgtttt
     2761 atatagatgt tgacaaattt aaatatataa atgatatgtt tggatatgat atgggaaatg
     2821 atactttgat acatatatca aatactattg caagtgaatt aaaggaagat gaaatttttg
     2881 caagagtttc tgctgaccat tttgttcttc ttattaagta taagacagat gatgatatta
     2941 aaacaaggtt aaacaatatt tacaataagg ttcaaatact tagtaatcct aaaataaact
     3001 attataaatt gattttagat tgtggtatat ataaaataag taaaagtgat aatgacataa
     3061 atactattat ggatagagct aatactgcaa gaaaaactat aaaaggtggt cataaaaatt
     3121 catttgcttt ttacgataag gaaatgcaca aaaaaatatt aaaagagaaa gaaattgaaa
     3181 attcaatggt agatgctctt aataatggag aatttattgt ttattttcaa cctaaatata
     3241 gcctttcaga ttatcaaatt ataggggcag aggctcttgt tcgttgggat aatccacaaa
     3301 aaggtctaat accaccaata gaatttattc ctgtatttga gaggaatggt tttattgtaa
     3361 atattgattt ctatgtattt gaggaagtgt gtaaaaagat tagagaatgg atggatgaag
     3421 gacaaaaagt agtacctata tctgtaaact tgtctcgaat gcattttgta aatagtaatt
     3481 ttatagagga gtttaaatta attgtagata aatacaaaat tccgacaaga cttatagaat
     3541 tagagctaac agaaacagca gtattagata atatagaagg attacttgat acaatgaaca
     3601 atttgaaaga aaagggattt gtaatctcta tggacgattt tggtacagga tattcctctc
     3661 ttaatctttt aaaagaattg cctgttgaca tattaaaact agatagagca ttttttacag
     3721 aaaaagatga gagtaataat gaaaaaattg ttatatctaa tgtcattaaa atggcaaagg
     3781 aactgaaaat gaaagtaata tcagaaggtg tagagactat ttcgcaagta gaatttctaa
     3841 agcaaattgg gtgtgatatg gtacaaggat atttgttttc taagccaatg cctgtaaaag
     3901 agtttgaaaa aatcgctttt aaaaaagaat aaagcaatgt gtaaataatg acatatataa
     3961 taaaggtgct ttaatattag ttgagtatac tattttagag catctttatt tttgtattaa
     4021 tttttacgat tttattaata aaataaatca aatgagaaaa aaaatcaaaa gaatacaata
     4081 atcacttgca aaaaatatac tattgtgata gacttaaatc aaaattagga attgtaaaaa
     4141 gaaggaaaaa ataggatatt attttaatag attaagattt aaggtattgt aatttatata
     4201 taagataacg attgcttgta gatttaaatt actttaagag gagggatatt atatgatgcc
     4261 attgatatta tcaaatacag gtgaagaagt agttataaag aaaatttcgg gaagtgataa
     4321 gacgaggact tttttaaata gtataggttt tgtagtgggt acaaatgtta aaatagtttc
     4381 taagatagat ggtaacttaa taatagacgt aaaagatact agagttgcat tagataaaaa
     4441 aatggcaagt agaataataa tatagggagg taatgacatg aacagactga aggatattaa
     4501 gtgtggagag actgtaaaag tcaaaaagtt agaaggagaa ggagctacac gaagacgtat
     4561 aatggatatg gggattacaa gaggtgttaa tatatttata agaaaagtag ctcctcttgg
     4621 tgacccaatt gaggtaacag tcagagatta tgaactatca attcgtaagt cagatgcaga
     4681 aaaaattatt gtggaataat aaagattaat tagagtaggg ttaatgtaat tatataatga
     4741 gaatgatttt ctatatcaca aacagtgatg tatttaaata ataatggagg gaatggaaat
     4801 gtcaataaaa ataggtctca taggaaatcc taactgtggt aagacaacaa tgttcaatgg
     4861 gcttacaggc tcatcacaat atgttggtaa ctggccagga gtaacagttg aaaaaaaagg
     4921 tggaaaatta aaaggaaata aagatgtgga aatagtagat ttaccaggta tttattcttt
     4981 atctccatat acattagaag aagtagttac acgtaatttt atgctagatg ataagccgga
     5041 tgcagtaata aatattgtag atgcatcaaa tatagagaga aatttatatc ttacaactca
     5101 agttttagag ctaggtatac cgaccgttat agctcttaat atgatggata ttgtaaataa
     5161 gaatggtgat aagataaata ttaaagaact atctgaagtt ataggttgcc cagtggtaga
     5221 agtaacagca gttaaaggtc aaggaattat ggaggctgct gaaaaggcag ttgaacttgc
     5281 aagtagtaac aataaattaa atttcaaatt accctttgtt gatgaaagta aggatgcaat
     5341 agaaaaaatt gaaaaaataa tagaagaaaa gactccatat atagatgtag aaacacgctg
     5401 gttagctata aagctatttg agagagatga aaatgttata caaaaacttg atatttctaa
     5461 aacaatattg aatagtattg aagaaataac tagaaattgt gaagatgaat tggatgatga
     5521 tagtgaaagt ataataacag ccaatcgtta tgaatttatt agttcaataa tttcaagtat
     5581 aataaagaag aatcgaaaag gtaaggaaac tgtatcagat aagatagata agattgtaac
     5641 aaatcgtata ctagcattac ctatttttgc acttataatg tggggagttt actatatagc
     5701 agtaagttca ttgggtacta tagcaactga ttggacaaat gatgtactgt ttggagaaat
     5761 tattcaaggc aatgtaagta attttctagc ttctttaaat gtagcagagt ggttacaagg
     5821 acttgtagtt gatggtctaa ttggtggagt tggagcagta ctaggatttg taccacaaat
     5881 catgctttta ttcttattat tatcaatatt agaagattgt gggtacatgt cacgtgtagc
     5941 ttttataatg gatagaattt tccgtaagtt tggtctttca ggaaaatcat ttataccaat
     6001 gcttataagt tcaggttgtg gagttccagg agttatgtca actcgtacta tagagaatga
     6061 tagagataga aaaatgacta ttatgttaac tacattcatt ccatgtggag ctaagattcc
     6121 aataatagca ttatttgcag gggcattatt tggaggtgct tcatgggtag ctccatcaat
     6181 gtatttctta ggtattgcaa tgataatcat ttgtggaatt atattaaaga aaactagttt
     6241 atttgcagga gaaccatctc catttgttat ggaattacct caatatcata ttccaagtgc
     6301 aaaaggtgtt ttaatacata tgtgggatag aggaaaagca tttataatca aagcaggaac
     6361 aattatattt gttgcttgtg gagtaatctg gttcttacaa tcatttaatt ggtcattaca
     6421 aatggtagat gctggagata gtatattagc tagcttagga aatatagttg caccaatatt
     6481 tgctcctctt ggatttggaa attggcaatc atcagtagca acagttacag gtctagtagc
     6541 taaggaaaat gtagttggaa catttggagt tttatttggt atatcagatg caacagagca
     6601 agacccaaca cttcttgctt cagttgcaag catgtttaca gtagctagtg cgtttgcatt
     6661 tatggcattt aatatgcttt gtgcaccttg ttttgcagct attggagcca taaaaagaga
     6721 aatgggttca tggaaatgga catggattac attaggattc caaactttaa cagcgtatat
     6781 aattgctctt cttataaatc aagttggaag tttagtttta ggtactggag gaagtattgc
     6841 tggggcaata atatctatat ttatagcagt agcagtagtc tttgtagttt taacttattc
     6901 aaataaaaat atgaaaaaag aaaaaatggg taaattaagt tatatgaaaa attaattcat
     6961 ataaataagg agtgatgaat atggctacat ttattatagc agctattgta gtagtattaa
     7021 tggcactagc tgtaatgtat atggttaaaa attcaaaaaa gaatggtagc agttgtggat
     7081 gtggttgttc tgaatgctca agtagtaaga gttgtcatag cgctaagaga taaataggat
     7141 aagagcctga taaaaagtta aattgcttta ttatcaggtt cttatttttt gtataaataa
     7201 caaatttcat atttgaagta ggttagacta tgctgtaatt ttactgtata attatatata
     7261 aatatatgat taggagggat tattgctaaa ttaagcaata taagtgaata ttatgtttaa
     7321 atttgatgtt acaaacgagc ttatgtgtat agcaagggga agtggtaaat tttttgcaaa
     7381 aaaaggtgct atggtagcct ttaaaggaaa ttttaatttt gaaaaattgc tgttaggtcc
     7441 aagtaatggc ggtggattgg gaagagcatt attaggtcat gttcagaggt cattgacagg
     7501 agaacaaatg ccaataatgg tggttgaagg tgaaggtgaa atttacctag ctcaaaatgc
     7561 ttaccatgta gatgtaatat caattgaccc aggtgattcc atttatgttg agagtgaaaa
     7621 cttattggca ttttctgaac aattaaatta cagtgtaaaa ttgattggtt caggggtaat
     7681 atctcaaaaa ggtcttttta ctacacattt agtaaataaa acaggtcaaa atcaagatgt
     7741 tgctataata acagatggta atcctctaat tcttgaagga ccttgttgtg ttgacccaga
     7801 tgcattagta gcttggactg gaagagaacc atatcctaaa gtatcaaaac tatcatggaa
     7861 gacttttatt ggtcagactt caggagaatc ttatcatatg gaatttgttg aacctggaca
     7921 aatagtcatt atacaaccat ctgaaagatt gtctggctta aaaacagatt ctacaccttc
     7981 tgatagaatg agttcaggtg gattaagact tggcatagat taaatcttat gatatagctt
     8041 aattattatt taagatatta aaaaataaaa agggtagcag ggatagatat gttaaaagga
     8101 gttttatatg tttgagaaaa ttcataaaag gcttaaagga tattatattt ttttaataat
     8161 tttaatttta tggaagatag tatgtgtttt gggtatatgg agtagctata ttctgcctcc
     8221 tcctgaaata gtcttagata cttttataaa aatgatatat gacggttcta ttttcttaaa
     8281 tgtttatgcc agtgtaagaa ggatattaat aggtttttta ataagttcat tgatggctat
     8341 accacttgga gttttatttg gagtaaataa aaaaatgtat gaatatttca aaccactatt
     8401 tgaatttatg cgaagtacac ctccactagc tttggtgcca atgcttattt tatggtttgg
     8461 tataggtgaa gaatctaaaa ttataattat tattttggca tccttttttc caatattttt
     8521 aaatacattg aaaggtataa aaaattgtga caataaatta atagaagtag gtaaatcttt
     8581 taatttatct aaaaagagaa ttttttataa aataataatc cctaattcta tgctggatat
     8641 tgttgttgga cttagtttag ggcttgggta tagttttagg gctataattg gagcagagct
     8701 tattgcagct tcttcaggac taggatattt gatttctgat ggaaaagata tgtctagaac
     8761 tgatgttgta ttggtaggaa tttttgttat tggattttta ggtatacttt cagattatct
     8821 attttcaaag ataataacaa aatttagcaa agggaagcag gttgatttaa atggttagaa
     8881 gtggattttt gctagagaat atatataaga aatatttagt tgataataaa gagcatttag
     8941 ttcttgataa tatatcttta aatatatcta gtgaagagat aactgttata ttaggtgaaa
     9001 gtggatgtgg gaaaacaacg ctacttagaa ttttagcagg gcttgaaaat gctactagtg
     9061 gaaacatata tttttttaat aatgataaaa agtgtactcc aaaagtagga atggtatttc
     9121 aagaaagtag acttatgcca tggctgaatg taagtgaaaa tatactttta catactgaaa
     9181 aagataatag aaataaagtt gatttagaca agtatttaaa aatgatgaag cttgaaaagt
     9241 ttaaaaactc atatccaaat gaattatcag gaggaatggc tcacagagta agtatagcaa
     9301 gagcactttc cttcaatccc gatattttgc tgatggacga accatttgca gcactagatt
     9361 acttcacaag aagaaaaatg caaaaagaag ttgtaaatat ccataaaaat actaaaaaag
     9421 gtgtagtatt tgtaactcac aatattgaag aagctatgga aatagcaaag aaaataattg
     9481 tttttagtaa aaataaaagg attaaacaat ttagtgtcga agatgaatat aatagagatt
     9541 taactaaaaa ctattatata aacttgaaaa aagaaatatt aagggaattg ggggagtttt
     9601 aattgattaa aagaaacaaa aagattttag cagtaatgac atctttaata atggtgttgg
     9661 ttgggactgt aggatgttca acaacaaatg ataagaaaga tgaacctgca aaaaaagagg
     9721 ctagtttacc taaggaaata aatttaactt atgtaaaatc accacttaat gttcctagta
     9781 tattagaaaa aaaggatgat ttatttggaa aggaatttaa aaaggacaat ataaaagtca
     9841 atttccatga attgacaaca ggaccagagc aaactcaagc attagcagca ggtgagctag
     9901 actttttaca tgcattagga ggaacgtctg caataatagc tgcatcaaat ggtgttggtc
     9961 ttaaaattac gaatatttat agccgttcac caaaaggata tatgcttata actaataatg
    10021 ataatattaa atctataaaa gacttaaaag gtaaaaaaat tgtaggtcca aaagggacta
    10081 tacttcatca actgttgatt gctgcacttg ctaaagaagg ctatactata gatgatgttg
    10141 aatatgtaaa tatggatatt cctagtgctg cttctgcatt agaaagtaaa agtgctgatg
    10201 tagcaatgct tgcaggtcca gtggcattga aaacgataaa ttcaggtgcc aaaatgattg
    10261 taaatggaga aggattagta agtggaataa tagttactgc tgtaagtgat gactttgctg
    10321 aaaaatatcc tgaattagta aaaagattta tgaaggttca tgaagaaact ttaaaatata
    10381 tgaatgaaaa caaggatgaa gtgatggatg ttgtatctaa agaagttggt ttatctttag
    10441 atgaaacaaa agaaatgtat tcttggtatg actttagttc taaaataacg gacaaggata
    10501 taaaagaatt agaagataca caggaatttt taatgtctaa tggaatgcaa caaaagaaaa
    10561 taaatattaa agatatgttg tacaatcaaa attagaatac tttaaaatat taaagtggaa
    10621 aatacaccct tatttattat ggaaactata tgataaaaga gggtgtattt ttattattat
    10681 acaaaaatac aattaaaaac agcttactat tttatctaca cttatgtagt ctagtaattt
    10741 tctcattttc ttacggtgaa gtaaaaactt cgaatatgtg tcctcggatt gttttgtgtc
    10801 accataaact ttccaatatt taatcataga aacttcataa ttactatatc ccataaatcc
    10861 aatcacatct ttaagaggat atcctaaaaa aatacctatt tcatcaggaa aaacatcact
    10921 cttaagttta tctactaaat gctctaaata agcattaaca ttcgtatttt gtttgtatcc
    10981 taaaaacttt aaaaagttta ctgttttttt acattttaat ttttctgata atgctttttt
    11041 atttacaaat aagattttta atcccttttt ttctttatca ataactataa aatctatttt
    11101 cttacattta tatagatatt tataaatatc attaattttt tcctctctat ttacatcagt
    11161 aaacgaaata tttattattt cagcaggttt agagcctaaa aagactggac ctaaaacttc
    11221 taaaagccat tttatatatg atgattcaat tttattttta caacaaaatt gtgaattaca
    11281 acaagacata aaagataacc tccttcaaat gaaaattaat atcaagtaat tagattatac
    11341 tatgaatgat aatcaatat
//
LOCUS       DAGSWI010000131        11014 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.132, whole genome shotgun sequence.
ACCESSION   DAGSWI010000131 DAGSWI010000000
VERSION     DAGSWI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.132"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            86..8218
                     /gene="tcdA"
                     /locus_tag="MJF80_003131"
                     /gene_synonym="toxA"
     CDS             86..8218
                     /gene="tcdA"
                     /locus_tag="MJF80_003131"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBZ0281771.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(8553..9251)
                     /gene="tcdC"
                     /locus_tag="MJF80_003132"
     CDS             complement(8553..9251)
                     /gene="tcdC"
                     /locus_tag="MJF80_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HBZ0281772.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPSCSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            9677..9922
                     /locus_tag="MJF80_003133"
     CDS             9677..9922
                     /locus_tag="MJF80_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBZ0281773.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(10140..10409)
                     /locus_tag="MJF80_003134"
     CDS             complement(10140..10409)
                     /locus_tag="MJF80_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBZ0281774.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT         4314 a         1390 c         1544 g         3766 t
ORIGIN      
        1 agaaaatcaa tttaaatttc agaaggaata aatgtggtta tagaagtgga tttattatca
       61 aaaataataa tactaggagg tttttatgtc tttaatatct aaagaagagt taataaaact
      121 cgcatatagc attagaccaa gagaaaatga gtataaaact atactaacta atttagacga
      181 atataataag ttaactacaa acaataatga aaataaatat ttacaattaa aaaaactaaa
      241 tgaatcaatt gatgttttta tgaataaata taaaacttca agcagaaata gagcactctc
      301 taatctaaaa aaagatatat taaaagaagt aattcttatt aaaaattcca atacaagccc
      361 tgtagaaaaa aatttacatt ttgtatggat aggtggagaa gtcagtgata ttgctcttga
      421 atacataaaa caatgggctg atattaatgc agaatataat attaaactgt ggtatgatag
      481 tgaagcattc ttagtaaata cactaaaaaa ggctatagtt gaatcttcta ccactgaagc
      541 attacagcta ctagaggaag agattcaaaa tcctcaattt gataatatga aattttacaa
      601 aaaaaggatg gaatttatat atgatagaca aaaaaggttt ataaattatt ataaatctca
      661 aatcaataaa cctacagtac ctacaataga tgatattata aagtctcatc tagtatctga
      721 atataataga gatgaaactg tattagaatc atatagaaca aattctttga gaaaaataaa
      781 tagtaatcat gggatagata tcagggctaa tagtttgttt acagaacaag agttattaaa
      841 tatttatagt caggagttgt taaatcgtgg aaatttagct gcagcatctg acatagtaag
      901 attattagcc ctaaaaaatt ttggcggagt atatttagat gttgatatgc ttccaggtat
      961 tcactctgat ttatttaaaa caatatctag acctagctct attggactag accgttggga
     1021 aatgataaaa ttagaggcta ttatgaagta taaaaaatat ataaataatt atacatcaga
     1081 aaactttgat aaacttgatc aacaattaaa agataatttt aaactcatta tagaaagtaa
     1141 aagtgaaaaa tctgagatat tttctaaatt agaaaattta aatgtatctg atcttgaaat
     1201 taaaatagct ttcgctttag gcagtgttat aaatcaagcc ttgatatcaa aacaaggttc
     1261 atatcttact aacctagtaa tagaacaagt aaaaaataga tatcaatttt taaaccaaca
     1321 ccttaaccca gccatagagt ctgataataa cttcacagat actactaaaa tttttcatga
     1381 ttcattattt aattcagcta ccgcagaaaa ctctatgttt ttaacaaaaa tagcaccata
     1441 cttacaagta ggttttatgc cagaagctcg ctccacaata agtttaagtg gtccaggagc
     1501 ttatgcgtca gcttactatg atttcataaa tttacaagaa aatactatag aaaaaacttt
     1561 aaaagcatca gatttaatag aatttaaatt cccagaaaat aatctatctc aattgacaga
     1621 acaagaaata aatagtctat ggagctttga tcaagcaagt gcaaaatatc aatttgagaa
     1681 atatgtaaga gattatactg gtggatctct ttctgaagac aatggggtag actttaataa
     1741 aaatactgcc ctcgacaaaa actatttatt aaataataaa attccatcaa acaatgtaga
     1801 agaagctgga agtaaaaatt atgttcatta tatcatacag ttacaaggag atgatataag
     1861 ttatgaagca acatgcaatt tattttctaa aaatcctaaa aatagtatta ttatacaacg
     1921 aaatatgaat gaaagtgcaa aaagctactt tttaagtgat gatggagaat ctattttaga
     1981 attaaataaa tataggatac ctgaaagatt aaaaaataag gaaaaagtaa aagtaacctt
     2041 tattggacat ggtaaagatg aattcaacac aagcgaattt gctagattaa gtgtagattc
     2101 actttccaat gagataagtt catttttaga taccataaaa ttagatatat cacctaaaaa
     2161 tgtagaagta aacttacttg gatgtaatat gtttagttat gattttaatg ttgaagaaac
     2221 ttatcctggg aagttgctat taagtattat ggacaaaatt acttccactt tacctgatgt
     2281 aaataaaaat tctattacta taggagcaaa tcaatatgaa gtaagaatta atagtgaggg
     2341 aagaaaagaa cttctggctc actcaggtaa atggataaat aaagaagaag ctattatgag
     2401 cgatttatct agtaaagaat acattttttt tgattctata gataataagc taaaagcaaa
     2461 gtccaagaat attccaggat tagcatcaat atcagaagat ataaaaacat tattacttga
     2521 tgcaagtgtt agtcctgata caaaatttat tttaaataat cttaagctta atattgaatc
     2581 ttctattggt gattacattt attatgaaaa attagagcct gttaaaaata taattcacaa
     2641 ttctatagat gatttaatag atgagttcaa tctacttgaa aatgtatctg atgaattata
     2701 tgaattaaaa aaattaaata atctagatga gaagtattta atatcttttg aagatatctc
     2761 aaaaaataat tcaacttact ctgtaagatt tattaacaaa agtaatggtg agtcagttta
     2821 tgtagaaaca gaaaaagaaa ttttttcaaa atatagcgaa catattacaa aagaaataag
     2881 tactataaag aatagtataa ttacagatgt taatggtaat ttattggata atatacagtt
     2941 agatcatact tctcaagtta atacattaaa cgcagcattc tttattcaat cattaataga
     3001 ttatagtagc aataaagatg tactgaatga tttaagtacc tcagttaagg ttcaacttta
     3061 tgctcaacta tttagtacag gtttaaatac tatatatgac tctatccaat tagtaaattt
     3121 aatatcaaat gcagtaaatg atactataaa tgtactacct acaataacag aggggatacc
     3181 tattgtatct actatattag acggaataaa cttaggtgca gcaattaagg aattactaga
     3241 cgaacatgac ccattactaa aaaaagaatt agaagctaag gtgggtgttt tagcaataaa
     3301 tatgtcatta tctatagctg caactgtagc ttcaattgtt ggaataggtg ctgaagttac
     3361 tattttctta ttacctatag ctggtatatc tgcaggaata ccttcattag ttaataatga
     3421 attaatattg catgataagg caacttcagt ggtaaactat tttaatcatt tgtctgaatc
     3481 taaaaaatat ggccctctta agacagaaga tgataaaatt ttagttccta ttgatgattt
     3541 agtaatatca gaaatagatt ttaataataa ttcgataaaa ctaggaacat gtaatatatt
     3601 agcaatggag gggggatcag gacacacagt gactggtaat atagatcact ttttctcatc
     3661 tccatctata agttctcata ttccttcatt atcaatttat tctgcaatag gtatagaaac
     3721 agaaaatcta gatttttcaa aaaaaataat gatgttacct aatgctcctt caagagtgtt
     3781 ttggtgggaa actggagcag ttccaggttt aagatcattg gaaaatgacg gaactagatt
     3841 acttgattca ataagagatt tatacccagg taaattttac tggagattct atgctttttt
     3901 cgattatgca ataactacat taaaaccagt ttatgaagac actaatatta aaattaaact
     3961 agataaagat actagaaact tcataatgcc aactataact actaacgaaa ttagaaacaa
     4021 attatcttat tcatttgatg gagcaggagg aacttactct ttattattat cttcatatcc
     4081 aatatcaacg aatataaatt tatctaaaga tgatttatgg atatttaata ttgataatga
     4141 agtaagagaa atatctatag aaaatggtac tattaaaaaa ggaaagttaa taaaagatgt
     4201 tttaagtaaa attgatataa ataaaaataa acttattata ggcaatcaaa caatagattt
     4261 ttcaggcgat atagataata aagatagata tatattcttg acttgtgagt tagatgataa
     4321 aattagttta ataatagaaa taaatcttgt tgcaaaatct tatagtttgt tattgtctgg
     4381 ggataaaaat tatttgatat ccaatttatc taatactatt gagaaaatca atactttagg
     4441 cctagatagt aaaaatatag cgtacaatta cactgatgaa tctaataata aatattttgg
     4501 agctatatct aaaacaagtc aaaaaagcat aatacattat aaaaaagaca gtaaaaatat
     4561 attagaattt tataatgaca gtacattaga atttaacagt aaagatttta ttgctgaaga
     4621 tataaatgta tttatgaaag atgatattaa tactataaca ggaaaatact atgttgataa
     4681 taatactgat aaaagtatag atttctctat ttctttagtt agtaaaaatc aagtaaaagt
     4741 aaatggatta tatttaaatg aatccgtata ctcatcttac cttgattttg tgaaaaattc
     4801 agatggacac cataatactt ctaattttat gaatttattt ttggacaata taagtttctg
     4861 gaaattgttt gggtttgaaa atataaattt tgtaatcgat aaatacttta cccttgttgg
     4921 taaaactaat cttggatatg tagaatttat ttgtgacaat aataaaaata tagatatata
     4981 ttttggtgaa tggaaaacat cgtcatctaa aagcactata tttagcggaa atggtagaaa
     5041 tgttgtagta gagcctatat ataatcctga tacgggtgaa gatatatcta cttcactaga
     5101 tttttcctat gaacctctct atggaataga tagatatatc aataaagtat tgatagcacc
     5161 tgatttatat acaagtttaa taaatattaa taccaattat tattcaaatg agtactaccc
     5221 tgagattata gttcttaacc caaatacatt ccacaaaaaa gtaaatataa atttagatag
     5281 ttcttctttt gagtataaat ggtctacaga aggaagtgac tttattttag ttagatactt
     5341 agaagaaagt aataaaaaaa tattacaaaa aataagaatc aaaggtatct tatctaatac
     5401 tcaatcattt aataaaatga gtatagattt taaagatatt aaaaaactat cattaggata
     5461 tataatgagt aattttaaat catttaattc tgaaaatgaa ttagatagag atcatttagg
     5521 atttaaaata atagataata aaacttatta ctatgatgaa gatagtaaat tagttaaagg
     5581 attaatcaat ataaataatt cattattcta ttttgatcct atagaattta acttagtaac
     5641 tggatggcaa actatcaatg gtaaaaaata ttattttgat ataaatactg gagcagcttt
     5701 aactagttat aaaattatta atggtaaaca cttttatttt aataatgatg gtgtgatgca
     5761 gttgggagta tttaaaggac ctgatggatt tgaatatttt gcacctgcca atactcaaaa
     5821 taataacata gaaggtcagg ctatagttta tcaaagtaaa ttcttaactt tgaatggcaa
     5881 aaaatattat tttgataatg actcaaaagc agtcactgga tggagaatta ttaacaatga
     5941 gaaatattac tttaatccta ataatgctat tgctgcagtc ggattgcaag taattgacaa
     6001 taataagtat tatttcaatc ctgacactgc tatcatctca aaaggttggc agactgttaa
     6061 tggtagtaga tactactttg atactgatac cgctattgcc tttaatggtt ataaaactat
     6121 tgatggtaaa cacttttatt ttgatagtga ttgtgtagtg aaaataggtg tgtttagtac
     6181 ctctaatgga tttgaatatt ttgcacctgc taatacttat aataataaca tagaaggtca
     6241 ggctatagtt tatcaaagta aattcttaac tttgaatggt aaaaaatatt actttgataa
     6301 taactcaaaa gcagttaccg gatggcaaac tattgatagt aaaaaatatt actttaatac
     6361 taacactgct gaagcagcta ctggatggca aactattgat ggtaaaaaat attactttaa
     6421 tactaacact gctgaagcag ctactggatg gcaaactatt gatggtaaaa aatattactt
     6481 taatactaac actgctatag cttcaactgg ttatacaatt attaatggta aacattttta
     6541 ttttaatact gatggtatta tgcagatagg agtgtttaaa ggacctaatg gatttgaata
     6601 ttttgcacct gctaatacgg atgctaacaa catagaaggt caagctatac tttaccaaaa
     6661 tgaattctta actttgaatg gtaaaaaata ttactttggt agtgactcaa aagcagttac
     6721 tggatggaga attattaaca ataagaaata ttactttaat cctaataatg ctattgctgc
     6781 aattcatcta tgcactataa ataatgacaa gtattacttt agttatgatg gaattcttca
     6841 aaatggatat attactattg aaagaaataa tttctatttt gatgctaata atgaatctaa
     6901 aatggtaaca ggagtattta aaggacctaa tggatttgag tattttgcac ctgctaatac
     6961 tcacaataat aacatagaag gtcaggctat agtttaccag aacaaattct taactttgaa
     7021 tggcaaaaaa tattattttg ataatgactc aaaagcagtt actggatggc aaaccattga
     7081 tggtaaaaaa tattacttta atcttaacac tgctgaagca gctactggat ggcaaactat
     7141 tgatggtaaa aaatattact ttaatcttaa cactgctgaa gcagctactg gatggcaaac
     7201 tattgatggt aaaaaatatt actttaatac taacactttc atagcctcaa ctggttatac
     7261 aagtattaat ggtaaacatt tttattttaa tactgatggt attatgcaga taggagtgtt
     7321 taaaggacct aatggatttg aatactttgc acctgctaat actcataata ataacataga
     7381 aggtcaagct atactttacc aaaataaatt cttaactttg aatggtaaaa aatattactt
     7441 tggtagtgac tcaaaagcag ttaccggatt gcgaactatt gatggtaaaa aatattactt
     7501 taatactaac actgctgttg cagttactgg atggcaaact attaatggta aaaaatacta
     7561 ctttaatact aacacttcta tagcttcaac tggttataca attattagtg gtaaacattt
     7621 ttattttaat actgatggta ttatgcagat aggagtgttt aaaggacctg atggatttga
     7681 atactttgca cctgctaata cagatgctaa caatatagaa ggtcaagcta tacgttatca
     7741 aaatagattc ctatatttac atgacaatat atattatttt ggtaataatt caaaagcggc
     7801 tactggttgg gtaactattg atggtaatag atattacttc gagcctaata cagctatggg
     7861 tgcgaatggt tataaaacta ttgataataa aaatttttac tttagaaatg gtttacctca
     7921 gataggagtg tttaaagggt ctaatggatt tgaatacttt gcacctgcta atacggatgc
     7981 taacaatata gaaggtcaag ctatacgtta tcaaaataga ttcctacatt tacttggaaa
     8041 aatatattac tttggtaata attcaaaagc agttactgga tggcaaacta ttaatggtaa
     8101 agtatattac tttatgcctg atactgctat ggctgcagct ggtggacttt tcgagattga
     8161 tggtgttata tatttctttg gtgttgatgg agtaaaagcc cctgggatat atggctaaaa
     8221 tatatgtttg ataaaaaatt attcctgtgc tactaagaaa ttatttttat ataataaata
     8281 ttgagattta attaaaagtc atgtgttatt gtaatacatg acttttagtt aaaatttttc
     8341 tatcatttaa taatctatta ttcttgacta ttttataata aaattcatat atggaaatat
     8401 taatactaaa taattaatag ttgataaaaa atagataata tgctaaaagc aaaaactaat
     8461 ttagagcctt gtaactgttt atttgcaatt ataaaaacat ctttaaacat attgactata
     8521 atataaaata ttaactataa tacaaaacaa tattaattaa ttttctctac agctatccct
     8581 ggtatagtta tatttccacc catagttgat tcataagtta tagttccagc acttatacct
     8641 cttatagtta tgtaatcatc ctctaatact cttgaaggag ttattgattt tttataacta
     8701 catagtacaa ccttatcata atttccagac acagctaatc ttatttgcac ctcatcacca
     8761 tcttcaataa cttgaataac cttaccttca aattttacct ttttgtactt ataatcatca
     8821 ggtgttctag ctaattggtc ataagtaata ccagtatcat atcctttctt ctcctcttct
     8881 tcttttttgc gttgttcttc agccttttta gcttcttcag cttttttagc ttcttcagct
     8941 ttacgttgat tttcagcttc aatagctttc ttttcgtcgt ctttcatttt gaaccatggt
     9001 tcagcatcag acaatttgct atttaaagtt tctatttctg ttttttgaga atctataact
     9061 gatgtttggt tagaaatgac ctcctcatgg tcttcagaac aagatggtga ggatatattg
     9121 ccaaaaaaca cgcctaaaat aaatgccagt agagcaatac cctttgtgct cttaaagaat
     9181 ttaattattt tcttagagct tccttttcct tcattactaa attcgttacc ctcatttttt
     9241 ttagaaaaca taatacaatc tcccttttat tagatatatt ttattttaaa agttaaaaac
     9301 ttaaaataac tagttgacta aaaattatat attcatattt tcctaaaata tatttcccct
     9361 aagcataatt ttacaaaata aagtaagaag attcaatata ttttgaaaaa ataagataaa
     9421 aatgtcagat tataatataa ttttcttgaa ggtgaatttt atatattaaa ttaaacataa
     9481 agtattttat ttgtataaaa ctatattatg atgtaattaa gtgactcagt gatttagagg
     9541 tggtaaaata tatatatatg aaagagcata aataaattga tataataaaa aattaaatat
     9601 taaatattaa ctataataaa aaaatatctg gtaataggta taatataaaa agaatatacc
     9661 agaaagaagg tgaagtatgg gagaactaca aaaaattcca ggggtaggga aagcaacaga
     9721 aaaatcttta atcatgttag gatatacaac tattaaatca cttaaagatg caaacccagc
     9781 acaaatgtat gaaaaggaat gtttaatgcg tggacagcac atagacagat gtcagttata
     9841 tgtatacaga tgtgcagtat attttgcatc aactgaaaat ccagaaccag aaaaattaaa
     9901 gtggtggtat tggaaagatt aaaaaatcaa aaaagttaga cataaaaaag agttgaatat
     9961 ccgttttaaa taataaaaac taatactcaa ctctttttta gtcgtaaatt ttatgtaaat
    10021 ctatttgtat ggctaaatat acccatcaat aaatgtataa atatactaat caacaaatga
    10081 ctaaacatac tacctaacaa aggattaaat aaattcctta acaaagcatt ctaaaacaat
    10141 taattagaaa ctgtttcttc aagccatttt ctacattgct caatatttac catagggtca
    10201 aaaccttggt cttttccctt atttaaaagc atatcacaag ggaaagtatc acattcacca
    10261 caatgattaa gttccttact ttcacagcaa gttttaacct tacattctcc accccaaaat
    10321 ggtttttcca tagttgggca accagtgcaa tttacttgtt ctttttttgt acaactgtca
    10381 caacagacac cacatcttga ttcaaacata ttcaaacatc ctttccatta tttaaaaata
    10441 ttataaaatg attttagatg aatacaaaag tgcaaatagt tgattttagg tcatctaaat
    10501 gttaaacata aaattattag tactggtaaa tttataaaaa ctattccaaa aatctttttg
    10561 aatatttcaa aacacaataa attacttcaa atccccttga atatatttta cagcattata
    10621 gattgcttta aatctctttg aatattctac aagactatag attagtttaa atccctttga
    10681 atatatttta caacattata gattgtttta aatctctttg aatattctac aagactatag
    10741 attagtttaa atccctttga atatttcaaa acaccataga ttactttaaa tccctttgaa
    10801 tgttctataa cactataaat tacttttaac ttttatataa ccataaagtt tttatctggc
    10861 actttaaatg gagttttata tcaaatttgc cttccatcga ataaccaaac ttaatttaat
    10921 tacacattcc aagattcaaa tattcattca atatatttga taattctatg ttatagctat
    10981 aattaaatat agattattaa agttaaatat ttat
//
LOCUS       DAGSWI010000132        10975 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.133, whole genome shotgun sequence.
ACCESSION   DAGSWI010000132 DAGSWI010000000
VERSION     DAGSWI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.133"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..594)
                     /locus_tag="MJF80_003135"
     CDS             complement(<1..594)
                     /locus_tag="MJF80_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBZ0281775.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNI"
     gene            complement(666..1019)
                     /locus_tag="MJF80_003136"
     CDS             complement(666..1019)
                     /locus_tag="MJF80_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBZ0281776.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1097..1300)
                     /locus_tag="MJF80_003137"
     CDS             complement(1097..1300)
                     /locus_tag="MJF80_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBZ0281777.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            1429..1638
                     /locus_tag="MJF80_003138"
     CDS             1429..1638
                     /locus_tag="MJF80_003138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281778.1"
                     /translation="MVQVTAKQPFQRLFTNHYIVALLIKVMAYSICWLSVSQTYLSTL
                     FSCQRTVGIDLPIKNHNSLWAFIDK"
     gene            complement(1690..1920)
                     /locus_tag="MJF80_003139"
     CDS             complement(1690..1920)
                     /locus_tag="MJF80_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0281779.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1922..2329)
                     /locus_tag="MJF80_003140"
     CDS             complement(1922..2329)
                     /locus_tag="MJF80_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBZ0281780.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            complement(2689..3138)
                     /locus_tag="MJF80_003141"
                     /pseudo
     CDS             complement(2689..3138)
                     /locus_tag="MJF80_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3152..4078)
                     /locus_tag="MJF80_003142"
     CDS             complement(3152..4078)
                     /locus_tag="MJF80_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281781.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            complement(4203..4985)
                     /locus_tag="MJF80_003143"
     CDS             complement(4203..4985)
                     /locus_tag="MJF80_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBZ0281782.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5141..5761)
                     /locus_tag="MJF80_003144"
     CDS             complement(5141..5761)
                     /locus_tag="MJF80_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBZ0281783.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5849..6547)
                     /locus_tag="MJF80_003145"
     CDS             complement(5849..6547)
                     /locus_tag="MJF80_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0281784.1"
                     /translation="MYKLSYVVRVSTKNLDELKRRCDEVKDFYDDLSIKLVRPFGDML
                     GLHGEFIPVSKRYINDYIQYVTSDFLAGLGFGATQTLGEHEDIYLGYNLDTGKNVYLK
                     PALASQGVKGSITNALASAFIGSLGGGKLFSNNMLVYYAVLFGGQAVIVDPKAERGKW
                     KETLPEIAHEINIVNLTSNDENKGLLDPYVILSRKKDSESLAIDILTFLTGISSRDSD
                     KFLQKNDNWKIIME"
     gene            6648..7463
                     /locus_tag="MJF80_003146"
                     /pseudo
     CDS             6648..7463
                     /locus_tag="MJF80_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8135..8902
                     /locus_tag="MJF80_003147"
     CDS             8135..8902
                     /locus_tag="MJF80_003147"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBZ0281785.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9230..9907)
                     /locus_tag="MJF80_003148"
     CDS             complement(9230..9907)
                     /locus_tag="MJF80_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281786.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10057..10374)
                     /locus_tag="MJF80_003149"
     CDS             complement(10057..10374)
                     /locus_tag="MJF80_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281787.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10396..10734)
                     /locus_tag="MJF80_003150"
     CDS             complement(10396..10734)
                     /locus_tag="MJF80_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281788.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3638 a         1737 c         1563 g         4037 t
ORIGIN      
        1 aatgtttgat tgaatttttt tatatggcca atcccaccat ttaatttcta ataattttga
       61 aattgtatca ttattaaatc tcttttttat aatttttgca ggtactcctc caacaataga
      121 atacggtgga acgtcattag taactactgc tcttgttcca ataattgcac catcaccaat
      181 atttacacct gacataatta tagaatcata acctatccac acatcatttc caattataat
      241 gttaccctta ttgtcccaag cgtccgttat atggttgata tcgagtttcc attcttcata
      301 aaagatggga aaagtatatg tggattttga tttcattgag tgatttccac tagtcattag
      361 aaattttgca ttacatgcta ttgagcaaaa cttaccaatt attaatttat cattgtttat
      421 agggtaatga tacaatacat tgtttttctc aaaatctctt gggtcattat aaaagtcatt
      481 atatattgtg taatctccaa cttctatatt ttcccttgtt attacatttt taagatatac
      541 agtttgataa tcattacttc ttggatatac cttatttgaa cttggaatag tcatatttaa
      601 atctctcctt taatatatat tatgtttttg actattccac tactacaata catctcttca
      661 cagaatcact ccttgttaag aatcttcata agataatggc gtcctttttt agtgttaagt
      721 cggatattct ttcttagtcc attcttttta gcgtataact gacaaactgt cattttctta
      781 ccaatagtgt caatgctatc atcaaggtca ttttgtattg ctttttcctt atcccttaat
      841 gatatatcat cacgttttcc ctttgggggt ttatcagtag gtacaagttt ccaagagtaa
      901 acaaattgtg gtttaccaaa tgaatataca tacttatatg cgtatcttcc atcttttctc
      961 tggctctctc cagttcttaa aattctatct ttattatctc gtcttttttc agttgacatg
     1021 ataagaagct cctctcaata accgaattga gcttgatacg tcatatgaat attataacat
     1081 ataaggctca atttcattag ataacgtcaa gtgtatcaac gattttctca aattgttttc
     1141 tctttatttg aatgcgattg ctattcataa tgacccagcc agaatcaagg ttttcatcag
     1201 cacgtttacg taattttttc tctccaatac gaaagtattt tgacgcttct tcaatcgtca
     1261 aggtatattt ttcccaaata ggtacatcag tattgttcat ataatgtatc actcctttct
     1321 atgttcgtag ttcagaagta gatagatggc tttgataagc tccattggta tttcaaccaa
     1381 tcccattctt atggacagaa ccgtgtcata caggtgtatc attatactgt ggtacaggtt
     1441 acggcaaaac aaccgttcca aaggttattc acaaatcact atatagtagc tctcttgatt
     1501 aaggtcatgg cgtatagtat ttgttggcta tctgtatcac aaacgtatct atctacttta
     1561 ttcagttgtc aaagaacagt cggcattgat ttgcctataa aaaatcacaa tagcttgtgg
     1621 gcttttatag acaaataatt agtgattctc catgtaccaa atagcttgat acatggagag
     1681 agggcgagat taagacaaat taaatctcaa aatagcagtg attagttttg ttcctagcat
     1741 tcgctttgtt tcttcttcta catgaatata gggatttccg tattcatcaa ataaagtttt
     1801 ctgtgaaaga cgaatgatat agtgttcaaa atgtttaagt attacgttta ttgcatttac
     1861 atcgccatgt acagcaagta aaataattgt aaaaggtaat acttctttag taggtttcat
     1921 ttcaatgttc ctgcatgagt tctttcaact cttttagtgc atttgttttg tgataatgta
     1981 tggtattttc tgctaaatcc atcaatgttg ctatgtcagc atttgtcatg ttgaaaaaga
     2041 atgaaagtag aattatatct tgttttcttt ttgtaagttt tccgattgct tgcgccatta
     2101 atacatcttc aattcgtatg ttgtagtcgt aagaaaccat acagatgtag ttagaatcat
     2161 aatcatctaa aatactcaat ttacttaatt ctgcaagtga caaagattcg agagatacca
     2221 aataatcatt tctgcgttta ttttcgtcat aaatatctct agcatagtta cgcaaaactg
     2281 ttttacagaa actgtcgaat tgatgttcaa ttgtcttttc atcaaccata agatttcacc
     2341 ctctttccat cgcaaggagt taaataacct tgcttactcc tatcacggtg ggagggcgtt
     2401 aaatcttcga taaatttcaa aaaaaagaga aaaaaataca cactctgcct atagggcaaa
     2461 aatgtgtatt ctttaaaacg tatttaattt atattggttg taagccacca ccatattgat
     2521 gtatggaaat atgcattggc tagcttttca taaaaataca aagatgcata agaaattaat
     2581 agaggggaaa aacttattct gttattaaaa agtaggttaa ttctaataaa tgctattgcg
     2641 gtcgaattaa actatataca tggcaatcaa tcatgtttct attctttatt atgaattgtg
     2701 acagtattct tcaatcttaa agccacattt ttccaatact ttacttgact ctctattgta
     2761 ggaatataca ttagcatgaa ttttgttaag gttgtatttt tcaaaggcaa ttttgcatat
     2821 ttcttttact gcatttgttg ctatcccttt gcctcaatag ttcctagcaa gccagtaacc
     2881 taattcagca tttttattaa tagaggtatc ttcttcaaat gttaggctga tacttccaat
     2941 agccctttct tgataaaaaa tagcatagct aagttgttta acatctgaag ttgccaaaca
     3001 aaaactaaca aaatctctag cgttttgctc cctataaggg tgggggaagt tgtcacgaag
     3061 attctttgca atttctttat cattcgcaaa ctcaactaaa tcggtaatat aagcataatt
     3121 ccactctata agttccattt ttcttctcct tttatgaaac agctacaaga aatctttcta
     3181 agccaatacc acttatcaaa cagcgttctc ttttatttcc aagcatttga taagtccagt
     3241 caacaaatcc accgtcaact atttcaacgg tttcatttcc ttgtttgacc gataatttga
     3301 aattgatacc tttataataa ttattatcaa catcatcatt atgaattgat aactctatat
     3361 taggaaaaat aagtttgata gtttcaacca ttctgtcgaa aaatccgtca ttatctttgt
     3421 atccgtttct tttgcgtaga ataatagaga aacgaacatt ttctatttca cttaatatat
     3481 ctttataata gtttaagtgc ttttctaaca acattttttc acaagtgtaa gaccctgtat
     3541 ctataccaga tgaaaccata caatataagc caaaatgtga atataaacct ttacctacaa
     3601 aattttgtgc tcttattacc ctagcagtag tagccatgtg gataggtatg gtattatttt
     3661 cttcttttct ttttaattta tcagcaataa ttattgccaa catatttgag gggtctgcaa
     3721 gtgtttctgt ccctcgtaga gcacttacta cgttatattg atctacggag ccaaaaacgc
     3781 tacagctacc taatggggca gagggagaaa gcataatcgt cttgatatcc atattgaatg
     3841 cttttctcaa taattttgat tccaaagcat gaatttcttc tggatctata ccgctaggga
     3901 tagtaaatct atttagttgg tatgacttta gaatatcagt tgcagttaaa gtatttgctt
     3961 gtctatcaaa cacttctaat agaagtgagt tcaaatctga ttttgataga gctgataact
     4021 tatcaactat attcttatcc ccaattttat ttgtaattct acttaaaata tcactcatag
     4081 ttatcctcca tactgttact ttcaaaataa ttatagaatt gttacaacaa ctaatctagg
     4141 tcatttgacc aaaaatgaaa aaacactgaa tatttactat tcaatatttt tttactttgg
     4201 gtttatttga ttaaatcttg aaaatactgt aatgcaaccc tttcagcttc aacctttcgg
     4261 acaataggac tttgtgataa ttcctctctt aactcaagat acaggttgat agtagcctgc
     4321 aatgcattaa cacagctttt atattcatgg ttagcaaccg tcttttctaa tgcttcaata
     4381 tattggggtg cgtccaattc aatttttcta actccacgag gtaatttacc taagagaagc
     4441 tgtattaatg gggcaagtac tgattgtctt aaaaaagata aaaaatcaat tgtttcaaat
     4501 agttctctac gtccaatctt agtgcaagcg taatgaaccc aaatccaaaa tctatcttca
     4561 atccactgta agtctggagt tgggaagtat gcttcttttt tagaataaat ttctgttatg
     4621 cagttgtttt cttcatagag aatagctgaa tcttcaaccc tatctagtaa accatctaag
     4681 gttgtaaact taaaatcaac atgaagaaca ggggaatcat agagggaaat tatcaatcga
     4741 ggttctccaa cgtgttcgcc agtaaaagca gaaactaatg ttccaaactg ttccacaata
     4801 agtaatctct cttcgataac ttcttcatag gatttatcat ctgctacaat cagaaaatcc
     4861 aaatcagaat attcatctaa cgattttgtg atataagaac tgctaattgc aagtcctatt
     4921 atcctactat ctctttttga aaattctaat acgttttgta tcattttctg ttgtgtttta
     4981 gtcataatat aacacctctc actgtctttt attagctatc aatccaacat aattatgcat
     5041 aggagtatta tataatgaag aaataaaaaa aaataggtca cttgacccga aataaaaaag
     5101 cattgaataa ttattattca atactttttt gaaattatac ttatgttgta ggaagattct
     5161 ttaatacctc tcgctgatta attacactaa atcttttgtt ttcataagca attagatgtt
     5221 cttgtacaca ttcatttata gcacgattaa tgcttctaat tggagcgttt acttcgctag
     5281 caagctgttt tttggtaagc agatttaatt cacttctata atagtgtaag gatatacacc
     5341 tcagaagtcg ttctttaaca cttaaagatg ataattcttc aattaattca gcatttacaa
     5401 ctaatttact agatatttct cgaatcagaa attttgtagc ttcaaaatct ccccttaacc
     5461 agtttaaaaa atcatctcta tgaagtttat atacattaca atctgtgaaa gcgacaattt
     5521 caacgggttt tttcccatca ttaaattgtt ccatttctcc aaaaaaacta ccacttctat
     5581 aaagatgaat cgttgagatg tttcctttca catttaagat gaatgcttct gcaataccgt
     5641 caatcataag aattacatat tcattttctt gctcagcaaa aagaatagtc tctttctttt
     5701 tatacgtttt tttaacaagc ttctttttaa tatcttcatt cataaaatca attaaatgca
     5761 tattcatccc tccttaccaa atctttgatt aaattatatc atctttcaat tagttaggta
     5821 agatagtaaa aatatattta catagaaact attccattat tattttccag ttgtcatttt
     5881 tctgcaaaaa cttatcactg tcacgactgg agataccagt aaggaatgtt aggatatcaa
     5941 tcgctaaact ttcagaatcc tttttacggc tcaaaatgac gtaagggtca agcaatcctt
     6001 tgttttcatc attactggtt aaattgacaa tgtttatctc atgggctatt tctggtagcg
     6061 tttctttcca tttgccgcgt tctgctttag ggtcaactat gaccgcctgt ccgccgaata
     6121 gtaccgcata gtaaacaagc atattgttac tgaataattt accaccaccc aatgaaccga
     6181 taaacgcaga cgctaacgca ttggtaatag aaccctttac cccttgacta gcaagggcag
     6241 gttttagata gacattcttt ccagtgtcca gattgtagcc aagatagata tcctcatgtt
     6301 cgccaagggt ttgtgtcgct ccaaaaccaa gtccagccaa aaaatccgaa gtgacatatt
     6361 gaatataatc attgatgtaa cgtttgctga caggaataaa ttcgccatgc aatcccaaca
     6421 tatccccaaa gggacgcacg agcttgattg ataagtcgtc gtaaaagtct ttgacttcat
     6481 cacaacgcct ttttagctcg tcaagattct ttgtagatac acggacaaca tagctaagtt
     6541 tatacataga ttctttacag ttaaaagtct gtttttaaaa aaatgtataa attataaatc
     6601 aaaatataga tactatcaat gacccactag gtgcaaaaag cattgatatg gttaaattat
     6661 cagacgctaa aagctgggct atccgaatga gtgaagatgg gtgtgcttat aaatccattg
     6721 acaattataa gcgctcactg aaagcgtcgt ttttcatggc aattcaagac gactgtatta
     6781 gaaagaatcc atttaatttt tagttaagtg atgttttgga agatacaaca gaaccaaaaa
     6841 tcgctctaac accagaacag gaaatgggct tattatcctt tatggggagc gataagactt
     6901 attgtaaata ttatgatgat gttgttatcc tcttagaaac aggactacgc atttctgaat
     6961 tatgcggttt gactttagaa ttggatatga aaaataaagc cattatggta gaccatcaat
     7021 tgttgagtga tactaagatt ggctattatg tagaagttcc aaagaccaca cagggtaagc
     7081 gagaaattcc aatgactgaa agagcttatc aatcatttga acgtatcgtg gaacggcgta
     7141 agaacgctaa agcagaacca atcattgttg atggttacca gaacttccta tttcttaaac
     7201 aagatggatt accaaaacaa gcaactcatt atcaaagcat gttaagagcg ttagtcaaga
     7261 agtataataa gacccatgag gacaagctac caaagattac gccacatacc tttaggcata
     7321 cactctgtac aaatatggct aacaaaggaa tgagtcctaa tatattgcaa tatattatgg
     7381 ggcataaaaa cattactatg acgcttggct attatgcaca tggttcattt agttcttcga
     7441 gagctgaaat ggaacggtta taatcctagt agtatgacta gagtttacta cttatttact
     7501 acttttgatg gcattcttat acggttaaat gccaacttat gcaaagtatc ttccaaaaag
     7561 aaaatgccga tactccttgt agatacagga tatatcgaca tttaccaact tatgaaaaga
     7621 taatcaaatt taatataagt ttttcaaata aaaagatacc aaattctttt tcgagaatct
     7681 atataacatt acttaagctt ggttggggta taaacaattg agtagctact tttgtatggt
     7741 gttcaacttt agtcaaaact cttaagtaat gcaaatgatt tagattcatt aatattcaat
     7801 ctcttcataa gtagtgaaat tcgagttatt tctgcataat tatactatga tttatagatt
     7861 aattctatta gcatccagaa taatattgtt tatatgagta aaatttaaag aatattgtga
     7921 aaattatagg atgttaaaat aaatttaggt aaaaaagaag tgtttgtata ttatagagta
     7981 ccttttctaa aatatgtggg attgttttaa gaattagaat aaaaattatt tttaaattag
     8041 tatttttata atgtatagta tttttaatgt ataattaaat tgtgcagaaa atagaattta
     8101 aagtcctata gaatttctga ttatggagga gaatatgaaa agaaaagttc aagtaaaaaa
     8161 tattactatt ggagaaggta gacctaaaat ttgtgtacca attattggga aaaataaaaa
     8221 agatattata aaagaggcta aagagttaaa agatgcatgt ttggatatta tagagtggcg
     8281 agttgatttt tttgagaatg tagaaaatat taaagaagtt aaggaagttc tctatgaatt
     8341 aagaagttat atacatgata tccctttatt attcacattt agaagtgtag tagaaggggg
     8401 agaaaaatta atatcaagag attattatac tactttgaat aaagaaattt ctaatacagg
     8461 tttagtagat ttaatagatg tagagttatt tatgggagat gaggtcatag atgaagtagt
     8521 taattttgct cataaaaaag aagtgaaagt aattatatct aatcatgatt ttaataaaac
     8581 tccaaaaaaa gaagagattg tatctcgttt atgtagaatg caggaattgg gcgcagattt
     8641 acccaaaata gctgttatgc cgcaaaatga aaaagatgtg ttagtgctac ttgaagctac
     8701 aaatgaaatg tttaaaattt atgcagatag accaataata acaatgtcaa tgtcaggaat
     8761 gggtgttata agtagattgt gtggtgaaat atttggttct gcattgactt ttggagctgc
     8821 aaaaagtgtt tcagcaccag ggcaaatatc atttaaagag ttaaattcag tacttaatct
     8881 attacataag agtattaatt aaattaatta tatagaaata ttagacggta tttttgaaga
     8941 tataaattaa atataaaaaa cttttcagat agtaaaattt aaattgggca attatatctg
     9001 aaaagcaact atttagtgtg tattttgaaa gtgatataga aaatcttata aatcaactat
     9061 ggcatattta aaatatgcca ttaaaaccta ctagacatta gattatagat ggtatactag
     9121 catttctatt atttatagat agtaagtttt cataataact gttttatcta aagtagaaga
     9181 tatatttgta ttatgtatat ttgtcattat ttttttgtaa ttatgtatat taatggttat
     9241 atatgtaata taatattatc actctatttt ttgtatattg tacattacaa catttatatc
     9301 tatcatcatc aattacagta gaatgtatgg gatacttatt ttcacttaac caatattttt
     9361 gcatattctt tgtccaagac tttggaaact tcaatacttt cttgtttatt ataacatcct
     9421 catcttcaaa aatattaact tctctttcta tttttatttt tctgttgtat atcttattga
     9481 tagattcttt gtcgagtttt atgtctctat acatatcagg gcaatagcaa caaacataat
     9541 ttcttttttt tcgacgttta aatttcacaa ctttctctat gctaatttct aatttttttg
     9601 taccatcttt tataaattta atatcttcta tgttctcgct tgaaaaaatt tctgtcaatc
     9661 ttaaagtatc tacagtttct ctaattattt gctttttata agaagagaat ccatatccaa
     9721 aagaaaggtt taatttatta gggatgtagc catttttata tttaaacatc ttaatttttt
     9781 ccaattcagc attatcttta aatttcaact tttcatacct aaaagttact ttgctaaaaa
     9841 gagtatattc tacatcaata tattcttttg caatcttaaa tttatcgcta ccttcaatca
     9901 caatcatagc ctatctccaa tcgttttata ttaaaataag gccatagaac atggtctaat
     9961 agtactcatt agcactataa gacaatgtta acacaaaaag acaaaaaaag gttatttatt
    10021 tcaaataacc ttttttttta tataaaatat aaagaattat attttattct tttttatgat
    10081 gtctataatc tgtgatatca gatttagttc tcttgaattt aagtctttta actcttcatt
    10141 tatatcattt tctaaatatg tttttccagt ggaagtaata tcttttataa gaacatctgc
    10201 agaaatattt aatatattta caaattttat gaatgtttta agacttggat tctttttacc
    10261 actttcgatt tgagttatat accaaggcga tactccaacc atatttgcaa attcttttga
    10321 agttaaattt gcttttttcc gttcttcttt aagtctttta cctatataat tcattattgt
    10381 tactatttcc ctcacttata tttctttgta tttaatgatg cactttatta actctagcat
    10441 tgcaatttgt tctttttcag ttaatttatt aatcatttca taacattcta aaggtacaga
    10501 gttatcaacg ccttctgttg taataatatt tgcaaataac aattcaagtg gtatgtctaa
    10561 agcagtagca attttcatta taatattgat agatgtattt gtctcagctc tttcaatctg
    10621 accaatatat gttggatgta aacctgcctt ttctgctaga aactcttgac tatatcccaa
    10681 ttttgttcta tagactttta ttctatctcc tattattttt cctatatttg ccaattatta
    10741 tccctccact ttagattata ttcatagttt aagttgtttt atatagacta tttatacttt
    10801 tttagtctaa atatagacta tatatgtttt tatactgaaa taagtgctat ttatttttgt
    10861 atagtctgta tctacaataa acaaatatgg ataaatcata tttttaatta gagaaatatt
    10921 ttcataattt catagccata ctacaataat aaaacgaatt aataatatgt taatt
//
LOCUS       DAGSWI010000133        10740 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.134, whole genome shotgun sequence.
ACCESSION   DAGSWI010000133 DAGSWI010000000
VERSION     DAGSWI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.134"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            144..608
                     /locus_tag="MJF80_003151"
     CDS             144..608
                     /locus_tag="MJF80_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBZ0281789.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            615..1109
                     /locus_tag="MJF80_003152"
     CDS             615..1109
                     /locus_tag="MJF80_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBZ0281790.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNVFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1125..2150
                     /locus_tag="MJF80_003153"
     CDS             1125..2150
                     /locus_tag="MJF80_003153"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBZ0281791.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2137..4584
                     /locus_tag="MJF80_003154"
     CDS             2137..4584
                     /locus_tag="MJF80_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBZ0281792.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4764..6137
                     /gene="radA"
                     /locus_tag="MJF80_003155"
     CDS             4764..6137
                     /gene="radA"
                     /locus_tag="MJF80_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBZ0281793.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6140..7210
                     /gene="disA"
                     /locus_tag="MJF80_003156"
     CDS             6140..7210
                     /gene="disA"
                     /locus_tag="MJF80_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBZ0281794.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7299..8393
                     /locus_tag="MJF80_003157"
     CDS             7299..8393
                     /locus_tag="MJF80_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBZ0281795.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8522..10699
                     /locus_tag="MJF80_003158"
     CDS             8522..10699
                     /locus_tag="MJF80_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281796.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         4485 a         1086 c         1995 g         3174 t
ORIGIN      
        1 cataattaat gaaagaataa attcatatct aaatttctat aaaaaattta aatttataca
       61 atttagggct ttaaaaattt aaataatagg tgtaaaataa tagtcaaaga aagtcaaaaa
      121 taaaaaggtg tgtggtgata ttaatggcaa ctatgacaga tataattgaa aaatttatta
      181 aagatttgat ggaagaagat aatagcatac aaattcaaag aaatgaacta gcaaacttgt
      241 ttagttgtgc accatcacaa ataaattatg tcttaacaac aagatttaca atagatagag
      301 gatattacat tgaaagtaaa aaaggtggcg gaggatatgt tcaaatagaa aaaatacgaa
      361 aaagtaaaga tgggcatatt agagaactac taaatgaaaa aataggaagt cagatatcct
      421 ataaaaaagc aaaagaatta ttagaaggtt taaaagagtc ggatttgatt aacgaaagag
      481 aattaaaatt aatattatat gcaatagatg ataaatcatt gtgtatgcca atatatgaat
      541 taaaagagaa ggtaaggtct aatattttaa agaatataat aataggatta tttagtatag
      601 aggagtgata atttatgcta tgtcaaaaat gcaataagaa taaagcatct gtatattata
      661 ataagatagt caatggagaa aaaacagaga tgtatttatg tagtgagtgt gcaaaagaga
      721 atacagaaat gaattttaac ttagatatgc ctttttcaat gatggatata ttttctaatt
      781 taggttttca acctaaaaaa gaacttgagg aaaagctagt ttgtcctaaa tgcaatacaa
      841 catatagtga gtttaagaat aatgggagat ttggatgtag tgaatgttat aatgtattta
      901 gtagtcaagt aaatccaatg cttcaaaata tacatgggca tatagaacat acaggaaaag
      961 cacctaaaaa atctttttat aagataagtg tagaaaatga gattaaagaa ctaaaagaag
     1021 atttagatag agctataaaa aatgaagagt atgaattagc agctcaattt agagataaaa
     1081 ttaaatattt aaaaggttct attgactaag attggagtga tattatgaaa gagaacatag
     1141 tcatgaaatc aagagttagg ctagctagaa atttaaataa ctatccattt ccaaataaat
     1201 tagataaaga atgtgccatg gaaataatag aaaaagtaaa aaatgcgttt attaattcta
     1261 acttagaaca aaaagaagaa tttgatttct ataaaattga agatttagac caatctaaaa
     1321 aaatgcttat ggttgaagaa catataataa gcccagattt agcagaaaat gataaaagtg
     1381 cagttatagt aaaaaaagat aaaactataa gtataatgat aaatgaagaa gaccatataa
     1441 ggatacaaac tatatgtgat gatttaaact tagaatatgc atatagtgta gcaaatgaaa
     1501 tagatgattt gttagaatca agtttagaat atgcctttaa cacaaagcta ggttatctaa
     1561 catcttgtcc aactaataca ggaacaggaa tgagagcttc tgtaatgatg catttaccag
     1621 cattaagtca actaggctat atggatgaac tttataaaat ttcatcacaa ataggtatag
     1681 caattagagg gatatatgga gaacgaacag aagcattggg aaatatatat caaatatcta
     1741 atcaacttac acttggaaga acagagtcta atattataga aaatgtaagt ggacttacaa
     1801 aagatgcaat ttcaaaagaa ataaaagcta gagaaatatt gcaaaaaaaa ttaggtataa
     1861 aactagaaga taaaatattt agatcgatag ggacacttga aaattcaaga gtaatgagta
     1921 gcgcagaggc aatgagccat ctatcaaata taaaaatggg aatagaaatg aactatatag
     1981 ataaactaga tttaagggct atagaacaac ttatgatagg tatacaacca gcgcatcagt
     2041 caataatgta taagagtgat gatgttgaga atagagatat aaatagagca acatatatta
     2101 gagaaacttt ggaaaaatta agaggaggga tgaattgtga acttcaatag atttacacaa
     2161 agagctaaaa aggcaattga tttagcgttt gaatctgcta aaagtctagg acacaatatt
     2221 gtcggaagtg aacatatact tttaggtctt ttaagagaag aagaaggtat agctgctaaa
     2281 gttctaagta aagttggatt tacagaagct tatttagaag gcaaaatagt tgatatggaa
     2341 ggtaagggag aagaaatctc agaagatata gtattaagtc caagaagtaa acaaatactt
     2401 gaattatcag gaatgtttgc aaataaatta aaaacaaatt atattgggac tgagcatata
     2461 ttattagcaa ttattcaaga aggtgaagga atagctaata agattttaaa ttatgctgga
     2521 gtaaatgata ggactttagc ccaattaaca attgatatga tgggtattag tgataaaaat
     2581 caatacaaag cagaaaatag ttacacgggc aaccagaatc aagcagaatc taaagtactt
     2641 gataagtatg gaagaaatct tacactatat gcaaaacaga ataaaataga ccctgtaata
     2701 ggtagagaaa aagaaataca aagagtaata caaatattaa gtagaagaac taaaaacaat
     2761 ccagtgttga taggagaccc aggtgtaggg aaaacagcca tagctgaagg tctggctaca
     2821 aatatagcct taggaaatgt tccagaaaca ctaaaaaaca aaactttata ctctttagag
     2881 atgggttcat tattggctgg ggctaagtat agaggagaat ttgaagaaag aattaaagaa
     2941 gttgtagatg aagttgttaa aaatggaaac ataatcttat ttatagatga gatgcacaca
     3001 ataatagggg ctgggtctac tggagaaggc tctatagatg cttcaaatat attaaagcca
     3061 gccttagcaa gaggagaaat acaagttata ggtgcaacta caattgatga atatagaaag
     3121 catgttgaaa aagattctgc tctagaaaga agattccaac cagttatggt agatgaacca
     3181 actaaagagg actcaataaa aatattagaa ggattaagag ataaatatga agctcatcat
     3241 aaagttaaaa taactgatga tgctataaag gcagctgtag aattatcaac tagatatata
     3301 tcagacagat atctgccaga taaagctata gacttgattg atgaagctgc atcaaaagta
     3361 agattaaaag aaaatacacc tccttcagaa ataaaaaaat tagaactaga aatagaaaat
     3421 atagataaag aaaaagaaga agcagtaaga tgtcaagact ttgagaaagc agccaaaata
     3481 agagatgaac aaggtctact taaaaaacaa ttagaagatg ttagagaaag atggaacaag
     3541 tcatctaaac attcagattt agttgatgga gaagttattg ctgaagtagt gggcttatgg
     3601 acaggaatac cagttaataa aattcttgaa gaagaggctg atagactttt gagacttgag
     3661 gaaatactgc acaatagagt tataggtcaa gaacaagcag taaaatctat ttcaaaggca
     3721 atcagaaggt caagagcagg tcttaaagac cctaatagac caattggttc atttttattt
     3781 ttaggtccaa caggagtagg taaaacagaa ttatctaagg cattagcaga agtgcaattt
     3841 ggagatgaaa atcaaataat tagaatagat atgtctgaat atatggaaaa acatgctgtg
     3901 tcaagaatga taggttcacc tccaggttat gtaggtcatg atgaaggtgg tcaattaact
     3961 gaaaaagtaa gaagaaatcc atattcagtt atattatttg atgagataga aaaagctcat
     4021 ccagatgtat ttaatattct gttacaaatt ctagatgatg gtagactaac tgattcaaaa
     4081 ggaagaactg tagattttaa gaatactatt gtaataatga catcaaatgt tggagcatct
     4141 acaattggta gacaaaaaac tttaggattt agtatagcta aaggagacga agaagaaaaa
     4201 tctcaatatg aaaaaatgaa agaaaatata atgggtgaat taaaacaaag attcagacca
     4261 gagtttttaa acagaataga tgatataatt gttttccact cactaaatga aaatcatata
     4321 tcaaaaatag tacttttaat ggcagcgaaa ctacaagaga gattaaaaga aatggatata
     4381 aaattagaaa tgagtgatga ggctgttaaa ttaatttcta aatctggatt tgacttagaa
     4441 tatggagcaa gacctcttaa aagagcttta caaaaagagt tggaagatga gctatctgaa
     4501 gcaatcttaa aaggtaatgt gaaaaaggga agtaatgtag tagcaaaagt caaagatgaa
     4561 aaaatagttt ttgaaactaa ataatttaat agagttgcat tttaaaatat tgcaaatata
     4621 agttttataa tctaaacaaa attttaaggg caatgaatct ataataattc attgtcctta
     4681 attattttat ttaatcttta catattgtct ttcagatatg ctaaaattat agtatagaat
     4741 aaaaaattaa ggatgataaa agtatggcaa aaataaaaac aaaatatgta tgtcaatctt
     4801 gcggatatga aacagctaaa tggcttggta aatgtccaga atgtacaaaa tggaatacat
     4861 ttgtagaaga aatagaccaa aaaagcacca aaaaagaggt ctttataata gataaatctt
     4921 catctaaacc agtcagtata aactcaatag aaagtaaaga agaagagcgg tttacaacag
     4981 atataaatga gttagataga gtgcttggag gaggtatagt taaaggctct ttagtgcttg
     5041 ttggtggtga ccctggtata ggtaaatcaa cacttttaat ccaagtatct agtaatgttg
     5101 ccaatttagg aaaaactgtt ttatatataa ctggagaaga atcagaatct caaataaaaa
     5161 tgagagctaa aagattaggt attaactcag aaaatttata tatatttgct gaaaataatt
     5221 taagtataat tgaatcttat ttagaaagtg taaatccaga attaataatt ctagactcaa
     5281 ttcaaactgt cttcagtcca gaaatatcct cggcaccagg aactgtcagc caaattaaag
     5341 aaggtacttc aaaattcatg aaaatttcta aaaaaatggg aatttcaacg ttcatagttg
     5401 ggcatgttac aaaagaaggt tcactagcag gaccaaaatt attagagcat atggttgata
     5461 ctgttctata ttttgaagga gaaagatata atacttatag attagtaaga gctgttaaaa
     5521 atcgatttgg ttcaactaat gaacttggag tatttgaaat gagggattta ggattagtag
     5581 aacttgataa tccatctaaa attttaatct ctgaaaaacc aaaagatgta gcaggttcag
     5641 taataatatc tactgtagaa ggtacgagac caatgttact tgaattgcaa gcattagttt
     5701 ctcctacaag ttttggtata ccaaaaagaa catctacagg agtcgattac aatagagtag
     5761 gtatgttatt agcagtatta gaaaaacgtg ttggtttgca aatacaaaat caagatgtct
     5821 atataaacat agttggagga ataaagataa atgaaccctc aattgattta gggatagcca
     5881 tttctgtagc atctagtttt agaaatatac cgatagatga agatatagct gttacaggag
     5941 aagtgggact tacaggagaa gttagagcag taagctttat agaaaagaga atagctgagt
     6001 gtaaaaaact tggatttaaa aagatagtag ttcctagaag taattatgat gttgtaaaag
     6061 agaccaaagg catagaaata tggccagtag ataacttaag acaagctata aatatagtac
     6121 ttgggaggaa tcaataaata tggagaattt tctagataat aaaaatatgc tatatgcatt
     6181 aaaaatgata tctcctggaa ctccacttag attaggtcta aacaatgtac taagagctaa
     6241 gactggtgga ttaattgtaa ttgcaacaaa cgaagatgta atgaaaatag tagatggagg
     6301 atttgctata aatgcagaat attcaccatc atatctatat gaattagcta aaatggatgg
     6361 agctatagtt ttaagtggtg atgtaaagaa aatattattt gctaatgcac aacttatacc
     6421 tgactatttt atagaaacat cagagacagg aacaagacat agaacagcag aaagagtagc
     6481 aaaacaaact ggtgctatag tcataggaat ttcacaaaga agaaatgtta taacagttta
     6541 tagaggaaat gagaagtatg tagtcgaaga tatatctaag atatttacta aggcaaatca
     6601 ggctatacaa actctggaaa aatataagac agtattggac caagctgtaa caaatttaaa
     6661 tgccttagag tttaatgatt tggtaactat ttatgatgtt gcattagtca tgcaaaagat
     6721 ggaaatggta atgagagtta caagtataat tgaaaaatat gtgatagaat tgggtgatga
     6781 aggaacttta gtaagtatgc aattagaaga attaatgggt acaaccagaa tagaccagaa
     6841 attaatattc aaagattata ataaagaaaa cacagaaata aaagaactta tgaaaaaggt
     6901 caaaaattta aattcagaag aactaataga attggtcaat atggcaaaac tattagggta
     6961 tagtggtttt tcagaaagta tggatatgcc tataaaaaca agaggttata ggattcttag
     7021 caaaatacat agactaccaa cagcaataat agaaaactta gtaaattatt ttgaaaactt
     7081 tcaacaaatt ttagatgcat ctattgaaga attagatgag gttgaaggaa taggtgaaat
     7141 aagagcaaca tatataaaaa atggactcat aaaaatgaaa caattagtct tattagatag
     7201 acacatatga tgaattttaa atgaatttta ctactttttt cttgaatata atatacactt
     7261 tggtctataa tagataagta taactagggg gtgaagcatt gataaggaaa gtaacaagaa
     7321 tattgtttat tttacttgga tttacaatag gtataacaac ctatttaaca ttaatgaaag
     7381 attttgaaat attaacattt ggaaaagaaa catatggtta tatcgcagct gtagtagcag
     7441 gtataataat agccatttta ggatatctta tagaaccatg ggttgtaaat aaagtaaaag
     7501 aaattgctaa aatagtggat aaagaattgt caaaatatcc tcagactgat atattgttag
     7561 ggtctatggg tcttatagta ggatttgtta ttgcgtattt attaagtgga ttagtaaata
     7621 gaataccaat agtaggtgga atactatcct tactattata tttattccta ggatatcttg
     7681 gaatgaaagt tgcgcttaaa agtaaaaatg atttatttaa tattggaaaa ctaggaagat
     7741 tagcaaatcc tattaaggat aaagataagg ataaagaaaa taaaaaagaa gtaaaagcca
     7801 ttcctccaaa agtattagat acaagtgtaa taattgatgg aagaatagct gatatttgta
     7861 agactgggtt tatagaagga aaacttatta ttcctgcttt tgttttggag gaactaagac
     7921 atattgcaga ttcttcagat gatttaaaaa gagtaagagg tagaagaggt ctggatatat
     7981 taaatataat tcagaaagaa ttaaatatag aagttgaaat aagtgagaga gactttgatg
     8041 atatagcaga agttgatagt aaattattaa agcttgctca ggttttaaat ggaaaagttg
     8101 ttacaaatga ttataactta aataaagttg ctcaattcca aggtgttgaa gtattaaata
     8161 taaatgagtt agctaatgct ataaaaccag ttgctatacc aggtgaagat atggttgttc
     8221 aagtggttaa agaaggaaaa gaggctcaac aaggtgttgc atatctagat gatggaacta
     8281 tgatagttgt tgatggagga agaaaatata tgaatgagac tataaaagta ctcgttactt
     8341 ctgttcttca aactcctgca ggtagaatga tatttgcaaa acctaaaaat taatatgttc
     8401 aaagaaacct ataatttggg ctgtttattt taaatagatg gtccaaattt atttatttat
     8461 aacgataatg tttaatgtat tcttaaagat ggggatttaa atcagaaagc agggagaatt
     8521 tatgagaaaa aagattatat ctttcttagc aacatttatc ataatattaa catcagcaag
     8581 tcagtatagt tttgcagatg atatatcaac acgtggaaaa gtaatattta tagatatgaa
     8641 tagaactagt atgagtaata tgttaagaat aaaatcatta agagaagaac tagacaatag
     8701 agggtatatt ggcttgatga atataagagg cgataaagga agtgatgata gaaggtcata
     8761 tgctagtatg ggtgctggtg gtagagctaa tgtggctaat gaagaagata ttaattttga
     8821 aagctcgagt aaggatagaa atatagtttt tgaatcagct acaggaaaaa gtgcaaaagg
     8881 tataaataat ttaactataa ataagtctat aaatgaaaat ttaaactttg gagagtatgg
     8941 ttctgtatta gggtcattag gacaaagttt atctgataat ggattaaaag catctgtatt
     9001 gggtaattca gatataattg aaaatggtca acttataaaa aatagaaact tatgtctaac
     9061 tgcaatggat gaatatggaa gaatacctaa tggaaatgta gatactataa ataaaaaaga
     9121 cttatctatg ccttatggaa tatcaactga ttatgataaa ttgattgtag agactaaaga
     9181 agcttacaaa aataatgatg tgatttttgt ggagcttgga gacacatata gattagattt
     9241 atataaacca aatttaaatg aaaaaacata tgagagtatg aaagataaca tagaaaaaaa
     9301 tatagatata tatttaaaaa atatattttc tatggttgaa gaaaatgaca ctgtctacat
     9361 agcaagtgca tttccaagtg atttagatta taaaaataaa agaagacttt cgccaataat
     9421 aaaattaaat ggagaaggga aaggtatatt atcatcttca actactagaa gagaaggtat
     9481 tgtaacaaat ttagatgttg gggttgaaat attagacaac tttaatataa aaaatcaaaa
     9541 tatggttgga agaaaatatg aacttattaa cagagatgat aataaagaat ttttgatgga
     9601 tgaatatcaa aagatagtat ctatttcaag cataaggtct acaatactga atggttttgt
     9661 aagtatagtt ttcttgtcat ggatagttgc tatgattgca attttattta gaaagcatat
     9721 atcaaaaaat tataaagaaa caacattttt tatattaaag gaacttttaa aaataggaat
     9781 agtaatgcca ctgtcattta tgataacacc aataatgaat ttcaaaacac cattagcaat
     9841 atctttagga ataattataa taacacttac tatatatctt ataagtaaag ttttaataaa
     9901 gaacgattta aaaaacatgc tgttttttac tggattaacc atagtaatta tggttattga
     9961 tgctgggttt ggctcatatt taatgaaaag caatgttatg agttatgatt gtataattgg
    10021 agctagatat tatggagtag gaaatgagta tcaaggtgtg gcaatcggta gtgcaatatt
    10081 tacatttgca attttactta catataaaaa tataccaaag tggtctataa tagttttttc
    10141 tttggttatc ttaattacaa gtgcatctcc aattatggga gcaaatgttg gaagtgctat
    10201 ttctgaatgt gttgcatttt tactatttat actattgata tataatgtaa agatagattt
    10261 caaaaaaatc gtattattag gtatagcagt attatttgta ttaggagttt ttgtagcaat
    10321 agatatgatt ttgggaagta attcacattt aggtatgttt gtaaaagaaa tttactttaa
    10381 tggaccagga gaaataatac aaacatttag tagaaagata gagatgaatt taaagctagc
    10441 tcaaaccagt gcatgggtaa atatactgtt aactggaata ggtgtaattt tagttcttat
    10501 gattaatcag attagatact ttaagcaact gatggatgag tatccaattg tatttaaagg
    10561 atttatagca tcaatagcag gatgtttagt aactctttta gtgaatgact ctgggattgt
    10621 gtcatcagct acagccttta tttatgtaat tgtacctatg ataacattaa gtataaatct
    10681 cacagcatta aaagaataag tactgtatga ataatatgta aatattaata cattaattta
//
LOCUS       DAGSWI010000134        10734 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.135, whole genome shotgun sequence.
ACCESSION   DAGSWI010000134 DAGSWI010000000
VERSION     DAGSWI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10734
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.135"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            137..1198
                     /locus_tag="MJF80_003159"
     CDS             137..1198
                     /locus_tag="MJF80_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBZ0281797.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLTVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            1289..2902
                     /locus_tag="MJF80_003160"
     CDS             1289..2902
                     /locus_tag="MJF80_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBZ0281798.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            3233..5377
                     /locus_tag="MJF80_003161"
     CDS             3233..5377
                     /locus_tag="MJF80_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBZ0281799.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            5465..5800
                     /gene="spoIIAA"
                     /locus_tag="MJF80_003162"
     CDS             5465..5800
                     /gene="spoIIAA"
                     /locus_tag="MJF80_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBZ0281800.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            5814..6242
                     /locus_tag="MJF80_003163"
     CDS             5814..6242
                     /locus_tag="MJF80_003163"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBZ0281801.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            6265..7029
                     /gene="sigF"
                     /locus_tag="MJF80_003164"
     CDS             6265..7029
                     /gene="sigF"
                     /locus_tag="MJF80_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBZ0281802.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            7309..7746
                     /gene="spoVAC"
                     /locus_tag="MJF80_003165"
     CDS             7309..7746
                     /gene="spoVAC"
                     /locus_tag="MJF80_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBZ0281803.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            7913..8926
                     /gene="spoVAD"
                     /locus_tag="MJF80_003166"
     CDS             7913..8926
                     /gene="spoVAD"
                     /locus_tag="MJF80_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBZ0281804.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            8940..9302
                     /gene="spoVAE"
                     /locus_tag="MJF80_003167"
     CDS             8940..9302
                     /gene="spoVAE"
                     /locus_tag="MJF80_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBZ0281805.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            9485..10501
                     /locus_tag="MJF80_003168"
     CDS             9485..10501
                     /locus_tag="MJF80_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBZ0281806.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         4306 a         1107 c         1952 g         3369 t
ORIGIN      
        1 gctttaaagt ataaaacaga ttatatgtta ataatgtggt gaaattatac ccatatataa
       61 ataaaataaa acaaataaat agctaattaa ggagaaaata ttttttattt tttgtttagg
      121 ataaataaat tttattatgg aaaaaagaaa aagcatacat cttcacaaac tgaatattta
      181 tttaataggg ggaggtgata gattgtttat cacagtggta ttatttttag taggtttcct
      241 attaattact aaaggcgcag atatctttat aaattgtact gtagagatag gtaaaaaaac
      301 gaatatatca gaaattatac ttggagctac tatagttagt tttgcaacaa cattacctga
      361 atttacagtg tctctacttg cttctattga tggtcatact actatgagtt taggaaatgc
      421 agttggttct ataatatgta atacagggtt agcattaggg ttggttgttt ttataagtcc
      481 atttaatgta gataaaaaaa tgtttttttc taagtcttta ctattaatag tatccgttat
      541 agttttaatt ttattgagtt tagatggagt tataacaaga ggtgattcat tattacttat
      601 aataatatta atcttttata tggttaataa ttatagaagt gtcgttggaa aatctgatac
      661 taaaaataga aatattaaaa ataacactat taaagacata tctactaaaa aagggaaaaa
      721 ttataggggt ttttccgtat tagaaattgt aaaaatacta ttactttttg ctacaggatt
      781 gataatgatg ataataggtt cacaaatcct tatagagagt ggagtaataa tagctagttt
      841 tttaaatata cctcaaggta tagtaagttt aactataata gcacttggaa cttctctgcc
      901 agagatagtt tcgtcgatta ctgctataag aaaaaatcat catgaaatat cagtaggaaa
      961 tattttgggt gcaaacatat tgaatatagt atcagtaata gctgtatcag caataccaaa
     1021 taacatacca atactatctc aaaatagaca attggatata ccattcatga tattattatt
     1081 attaacagta ataataccaa cacttaaatc taataaactt agtagaatac aaggtatatt
     1141 gatgttattc acatattttc tgtacatatc aattttgtat ttcatgtata ttatttaact
     1201 atactgttta aaatattaat tgcagaatat cctaaaaaag tatataatgg ataggttaag
     1261 attaattgat aacggagagg aataatatat gaatataaca aagtttgaag atttaccaat
     1321 aagtgaggga ataaaaaaag ctatagctga aatggggttt gaagaaccat ctcctataca
     1381 agcacagtct atacctgcga ttttgtcagg taaagacgtt ataggacaag ctcaaactgg
     1441 tacgggaaaa acagctgctt ttagtatccc tatactagaa acaatagacc caaataatag
     1501 aagtttacaa gctgtagtgc tttgtccaac aagagaactg gcaatacaag tatcaacaga
     1561 aataagaaag ttagctaagt attcgcacgg aataaagaca ttacctatat atggtggtca
     1621 gccaatagat agacaaataa aatctctaaa gagtggtgtg caagttgtaa taggtacacc
     1681 aggacgtaca atagaccata tcaatcgtaa aacattaaag atggataatg taaaaatgat
     1741 tatattagat gaagcagatg aaatgctaga tatgggattt agagaagata tagaaatgat
     1801 tttaagtaag attccagaag aaagacaaac aactttcttt tcagctacta tgccaagagg
     1861 aattttggaa ttaactaaga gatatcaaaa agacccagag catataaaag ttgttagaaa
     1921 agaacttaca gtttcaaata caaagcaata ctatatagaa actaggtcat caaataaatt
     1981 agaagtatta tgtagattag tagatgtata tgaccctaaa ctatctgttg ttttctgtaa
     2041 tactaagaga aaagcagatg aattggtagg agatttacaa gcaagaggat attttgcaga
     2101 tgcattacat ggtgacttaa aacagactca aagagatata gtaatggata agtttagaaa
     2161 tggtacaata gatatacttg ttgctactga tgttgcagct agaggtatag atgttgatga
     2221 tgttgaatgt gtattcaact atgacttacc acaagatgaa gagtattacg ttcatagaat
     2281 tggtagaact ggtcgtgctg gtagagaagg tatgtcattt acttttgtat ttggaaaaga
     2341 aatgagaaag atgaaagata tagaaagata tactaaatct aaactaataa agcataatat
     2401 accaacaata actgatgttg aagagaaaaa agtaggtact ttctttgcac aagttaaaca
     2461 aactatagaa gaaggtcact taactaaaca attacaatgg ttagaaggtt tctgtaatga
     2521 tgaagattat gcaatggttg atatagctgc tgcacttgta aaactatctc taggtgaaga
     2581 aatgaaagaa gaaataatag aagaaaaacc tagaagagag cgtggagata gaaaaggtgg
     2641 tactggagct aaagatggta tgattagatt atttatcaat attggtagaa atcaaagagt
     2701 acaagcaaaa gatatagttg gtgccatagc tggagaagtt ggaataccag gtaaagtagt
     2761 aggtactata gatatatatg ataagtatac ttttgttgaa atacctaaaa aagatgcaaa
     2821 aacagtaatt gaaaagatga aagatattaa gataaaaggt aataagatta atatagaaaa
     2881 agcaaataag aagaaaaaat aacgtatagt atacaaaact ataattagaa aatatataaa
     2941 ataatatatt ggatatttaa aaagcctgat gtgttttcaa aacagatagt agataatctg
     3001 taagagttaa cagcataagg cttttcttgt tttatataag gaatctgaat aacttactgt
     3061 taaatctttg aattactgca cctcatttac gttaaggatg gagtattcta cctatttttg
     3121 ataaaatata tctatgtttt atatactgta gttttgaata tataaagttt aataaattat
     3181 agtatatatg ttaattttta ataaggtaag aaagtagaaa gaaggatatt ttatggatat
     3241 aataaaaaaa ttaagtcatg ggtgtacatt agattgtcat gactgttgta aattcaatgt
     3301 ttatactaaa ggaaataatg tagtaaaaat tgaaggagat aaaaatcatc cttatacaaa
     3361 aggttttata tgcaaaaaag ggatggccca tttagataga ttaaatcata aagatagaat
     3421 aaagactcct atgcttaaag ttgatggagt atggaaagag atttcttttg ataaagctat
     3481 agaaataatg gcagaaaagc ttacatacta taaagaaaag tatacatcaa agtctgtcat
     3541 gcattatgac caatatggaa gtgggtctgt gctaaaatac ataggagata tattttttaa
     3601 tttttatgga ggtgttagca gacataaagg tggtccctgt tggagtgcag ggatgcatgc
     3661 tcaaaaatat gattttggag tagctaagag tcatgctata gaagatatgt taaatagtaa
     3721 aagtatattt gtgtggggaa aaaatccagc atatactaca attcatacta tgcaaataat
     3781 taaaaaagct aaagaaaaag ggataaagat agtagtgatt gaccccatat atacaaaaac
     3841 agctcaaata gcagataagt atgtacaagt aaatcctgga acagatggag ctttagctat
     3901 agctatggca aaaattatag tagaagataa attatatgat gaagaatata taaactcgta
     3961 tgttattggt tttgaagaat ataaaaaata tttatcttca ttggaattaa gttttttaat
     4021 tgatgaatgt ggtgttaaag agaatgatat aagagagtta gtggatttat atactaacaa
     4081 atattcaagt ataaatgttg gatatgggct tcaaaaatat aaaaatggtg gaaacacaat
     4141 aagagcaata gatgctttag gtgctataac tggtcaaata ggatttagtg gtggaggggt
     4201 aaactatgct aataaagttt atccgagtgt tataaattct gacccttaca atagccaatc
     4261 atatggtgaa gatagggagt tttatgtaag taatatcagt aaatttatag aagaatcttt
     4321 aaaaaataca tcaaataaag taaattatgc atcagatgaa cttgatatga catcaaataa
     4381 agtaaattat gtatcagatg aacttgatat aacatcaaat aaaacagatt atatatcaaa
     4441 tgaattgtat aatttatcta ataagtcaat caaaggtaat attcctataa agatggctgt
     4501 aataactaaa agtaatatgc tgaatcaact gccagattta gtagaattgg agagggtgtt
     4561 ttcaaaaatt gaatttaaag tatgttttga tatgtttata actgatacag ctactttatg
     4621 tgacatgttt ataccgtgta caaatacact agagagtgaa gatataattt atagctcaat
     4681 gaccaatcca tatataacct ataatgaaag agctgtaaag ccagcacata aattgatgga
     4741 tgaatattac ttctttatgg agctagctaa aaagatggga ttaaatgatt atccttttgt
     4801 agaaaagaga acttatttag aaaaagtgat tgaaccactt aagcgttttg acaaaaatct
     4861 ggatatagaa aaacttaaaa acaattactt tactatacat aaccctgttg cttgggaaga
     4921 taagaagttt gagactccat ctggaaaata cgagctttat tcagaaagta taaaaaactt
     4981 gggaataagt ccaacacctg tatatataag caataaatat aaagaaatag aagataaaaa
     5041 tatttctttt agattattaa ctaatcatca tgcagatact ttatttagtc agcattttat
     5101 ggacaaaaaa tctatagctc aagcatatat aaatcggaga atggctaaaa aagtaggtat
     5161 tgaggacaaa gatattgtga tattgaggtc aaaaaaagca aaaataaatg tacaaataaa
     5221 tatagatgat ggagtaggta attatatagt aaaaatgtat gttggttggt ggaagaaaca
     5281 tggaaatcct aatagtttaa cagacactgg tatatcagat tttgggggtc aggtaacata
     5341 taatgaaagt atggtagaaa taatacgaca aaactagtta aatttcattg aaggaataaa
     5401 aatataatta tagaattgat taaaagttac tatttttaca aaactattga aggagggatt
     5461 tttgatggta aattattcct tagaacataa gaatctatac atagaattta tgtgctcaga
     5521 actagaccac catgtagcca atgaaataag ggaagaaata gataacttat taagtgtaaa
     5581 tcaagtaaaa aatgttgtat ttaattttga aaatattaat tttatggatt cttcaggtat
     5641 aggcgtcatc ataggtagat ataaaaagat ttctaatgaa gggggacgag tttcagtaat
     5701 taatataagt tcacgagtta agaagatttt tgatttatct gggttaaata aaattattgg
     5761 catttatgat acgtatgaag aagctctaag ttctttgtag ggaggagtat gtgatgaata
     5821 atattatgga agttaaattt tcggcaatat ctgaaaatga aagtttagca agagttatag
     5881 tagcatcctt tgcagctaag ttagacccaa cagtagatga gttagtagat ataaaaactg
     5941 ctgtctctga ggctgttacg aatgctataa tacatggata tgaagaagac agttctaagt
     6001 ttgttttctt aagatgtgag atagaaggaa atactataaa gattatagtt gaagatgaag
     6061 ggtatggaat agaaaatgtg gagaaggcaa tggaaccact ttatacatca aaaccagaac
     6121 ttgatagatc aggaatggga ttcacagtaa tgaaaagctt tatggacgat gtagaggtaa
     6181 gttctgtaaa agataatggg actagaattg aaatgactaa aaaaataaat gtgcctaaat
     6241 aaagtccaaa agtgaggttt tattatggaa gtaactgttg ccagagaaga aaaaaaacct
     6301 cttcttagtc atgaagagac tctagaactt attgaaaaag ttcaaaatgg agatgaagag
     6361 gcaaaagaaa tattaatatc aagcaattta ggtctagtta ggagcgttgt atctagattt
     6421 ttaaatatag gatatgatag agaagattta tttcagttag gttctatagg cttaataaaa
     6481 tcaatatata aatttgaccc aaaatttaat gtaaaattct ctacttatgc agtaccaatg
     6541 attttaggtg aaataaagag atacttaaga gatgatggta tgataaaagt atcaaggtcg
     6601 ttaaagcaaa tagctataaa ggcaaagatg gaaagtgaag ctttaactaa aaaattaggt
     6661 agagagccga gtatagaaga attagctaaa gcaatagatg tagaaaaaga agatttagta
     6721 atggctatgg aagctaattt caatgttgag tatttgcaag gagttattca tgaagaggaa
     6781 ggttctccaa tatgccttat tgataaaata agcatgaaag gagaaagtga agaagaaaaa
     6841 gtagtggata acatattgtt aaaagacata cttggaagac tggataagag agagcgtcaa
     6901 ataatagttc tcagatactt tgaagacatg actcaaagtg aaataggaga gatgctcaat
     6961 atatcacaag ttcaagtatc taggatagag aagaaagtat tgtctaagtt aaaagaatat
     7021 atatcgtgat aatacttatg atatgtagaa taataaaata taataaatat attactaaaa
     7081 taaaatgtaa taacttttta tgttaagttt ttaaattatt aaaagatatt aaaataataa
     7141 aaagcattaa aaaagtaaaa atataaaaac aaggtgtctt tatggaaaaa tagatttttt
     7201 ctttattagg catctttttt attttttgac caaactttag atagtcaaaa taaataatag
     7261 gtttattttt ttaaaattaa caaataatac tttagagagg tgtttagtat ggataaaaat
     7321 tataaaaaat atgtagacca aataagccca aaacctacat atttaaaaaa ctatacactt
     7381 gcatttattg taggtggaat tatttgtatg atagggcaag caataaatga tttgtatatg
     7441 aaagttggag gtcttgataa actaggtgct agttctgcaa catcaataac attaatattt
     7501 ataggggcat ttttaactgg attaggagtt tacgacctaa taggaaaaag agctggagct
     7561 ggttctataa ttccaataac aggttttgct aactcaatag tatctccagc aatggagtat
     7621 aaaagagaag gctatgtatt aggtgtaggt gcaaacttat ttaaaattgc aggaccagtt
     7681 ttagtttatg ggataggttc ttctatttta tgtggaatta tttattatat ttttaagatg
     7741 ttttagatga tttttattta atttaaattt tattaattat gaagaaggta taaaatctga
     7801 aaatagctac taaagtaact aattgaaaaa taattctgca gatttaatta atatttttaa
     7861 ttaatattaa aaatttgaat agtaaaatgc aaaattaaaa aggtgtgaaa atatgaaaaa
     7921 taaaagaatt ggaaaaagaa cagtcaagtt agaaaataaa ccaaccataa tatcaactgg
     7981 aactatagta ggtcctaagg aaggtgaagg accactaaag gactattttg atatgattat
     8041 gactgacgat ttatatggtg aaaagacttg ggaattagct gaaagtaaaa tggtagagac
     8101 tgcatctcaa caagctatcc aaaaggcagg taaaaaattg tcagatgtaa attatatgct
     8161 aggtggcgat ttaataaatc aaatagttcc agcatccttt gcagcaagag aactggctat
     8221 tccattttta ggtatatatg gagcttgttc tacaatggca gaaggtcttt gtataggttc
     8281 tatgcttata gatggtggtt ttgctgattt agtactgtca ggtacatcaa gtcactattg
     8341 tacagcagaa agacaattta gattcccact tgaacttgga aatcaaaaac caatgacagc
     8401 ccagtggaca gttacaggag caggaagtgt attattagct cctaatggag acggtccaaa
     8461 agtaaagtat gttactgttg gtaaagtaat agatgagggt attgatgatg gtaataatat
     8521 gggaccagct atggctccag cagcaataga tactatctac tcttactttg aagatactaa
     8581 tgatgaccca aatagctttg atataattgc tactggagat ttaggtactc ttggaaaaca
     8641 gatagcagaa gactttctaa aagaaaaagg tgtagatata tcaaaagttt atactgactg
     8701 tggtatagag atgtttaacc ttaaagaaca agatgtccac tgtggtggaa gtggatgtgg
     8761 gtgttcagct acagtgtttg caggatatat atatgataag ttgagaaaaa aagaatttaa
     8821 caaagtaatg cttgtatcaa caggagcact actttcaccg acaagtacac tacaaaagca
     8881 gacaatacca tgtgttgcac atgcagtagt aatagtaaat gagtaggaag tgatataata
     8941 tgttaatgga ctatgtaaga gtatttattg taggtggaat tatatgttta atagcccaag
     9001 taatgatgga ttatttcaaa atgcaaactc catatgttat ggtaacgtat gtaacaactg
     9061 gtgtagtttt aacttttcta ggactttatg agccattagt taagtttgca gcagcaggtg
     9121 caacagttcc tataatagga tttggatatt cacttgctac aggagtaata aaatcaattc
     9181 aaagtgatgg ttttttaggt atttttttag gaggtacaac tgctacagct ggtggaatag
     9241 cagcagctat attctttgga tatattatgt cagtagtatt tactccaaaa gcaaaaccat
     9301 aaatctttat taaaagaata ataagttttt gaaataataa ctttttataa agcaggtgga
     9361 tatcttttag attccacctg ttttttatat aaagaagcgt ttacataatt gaagttgaca
     9421 attatttaca acatagtgta gtattatatt atcaaaataa aatgaagtga agtaggggat
     9481 aaagatggta actattaaag atattgcaag gaatctaggt atcagttatt caactgtttc
     9541 taggtgctta aacaataatc ctaatgtatc tgaaaaaaca aaaaagaaag tggtagaaga
     9601 ggcaaataga ctagggttcc attttaatgt aaatgcaaga aatttagcaa aaaaagaaac
     9661 taacaggata ggggtaatct tttcaaataa ttttaatcat caagatacta gaaagttttt
     9721 tagtgatata atggatagtt ctataaattc tattgagact aataaatacg attttataat
     9781 acagcctaat aataacatta gtggtgatag taatgtatat aaaatggtaa atggacaaat
     9841 ggttgacgga ttagtaattg tgtctcaatc aataaaaaaa gaagagtatg attttttaaa
     9901 agacaataat tttccacatg tatttatata ttttaaacca tcttttatag gagaagtaga
     9961 taatttcttt tgggacgata acgtgtatgg aggttatata gctaccaagc atttaattga
    10021 ccatggtcat aaagatataa taacagtaac ttcagatgat aagagttcaa aaatgcatga
    10081 agatagaacc aagggatatt tgaatgctat ggaagaagca aatttaaaga cagaagtcat
    10141 taaatctaaa atggattttg aatctcaagt tgaattttta gaaaaataca ttgataaaat
    10201 aaaaaaagct tcagctatat ttgtacaaca agatgtacca gcgatttcta taatacaaga
    10261 gttaaaaact acttatggta taaatgtacc agaagatatt tccataatag ggtataataa
    10321 tattgaatta atatcatatt ttaggtcgca tttatcaaca atagatgacc caagagagca
    10381 agttataaaa aatggagtag atagtttagt aaatataata aataaaaaga gtacagaaca
    10441 cattccaaga aaattatatc caaggttaat aatacgaaat agcgttaaaa gaataaagta
    10501 gtaagctttt aagtatcaaa cgtttattaa aaatattata gattatgtga agttttttaa
    10561 caaataatat aaataaaaat taaaaggagt aggtgtatat aagtacattg tactccttta
    10621 tttattgaga tagtttttat aatatgaatg aataatttta ttcaattatt tgaaaatccg
    10681 ttattttaac aatataaatt aataaattat ttgtatttta tatcatgaat ctat
//
LOCUS       DAGSWI010000135        10686 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.136, whole genome shotgun sequence.
ACCESSION   DAGSWI010000135 DAGSWI010000000
VERSION     DAGSWI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.136"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            180..638
                     /locus_tag="MJF80_003169"
     CDS             180..638
                     /locus_tag="MJF80_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0281807.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMAIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            699..974
                     /locus_tag="MJF80_003170"
     CDS             699..974
                     /locus_tag="MJF80_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0281808.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1033..2307
                     /locus_tag="MJF80_003171"
     CDS             1033..2307
                     /locus_tag="MJF80_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBZ0281809.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2437..3489
                     /locus_tag="MJF80_003172"
     CDS             2437..3489
                     /locus_tag="MJF80_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBZ0281810.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DNHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDTNPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3489..4520
                     /locus_tag="MJF80_003173"
     CDS             3489..4520
                     /locus_tag="MJF80_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBZ0281811.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGTTVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4612..5424
                     /locus_tag="MJF80_003174"
     CDS             4612..5424
                     /locus_tag="MJF80_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBZ0281812.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5427..6347
                     /locus_tag="MJF80_003175"
     CDS             5427..6347
                     /locus_tag="MJF80_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBZ0281813.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6522..6968
                     /gene="rpiB"
                     /locus_tag="MJF80_003176"
     CDS             6522..6968
                     /gene="rpiB"
                     /locus_tag="MJF80_003176"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBZ0281814.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            6998..7663
                     /locus_tag="MJF80_003177"
     CDS             6998..7663
                     /locus_tag="MJF80_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBZ0281815.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7773..9275
                     /locus_tag="MJF80_003178"
     CDS             7773..9275
                     /locus_tag="MJF80_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBZ0281816.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9587..>10686)
                     /locus_tag="MJF80_003179"
     CDS             complement(9587..>10686)
                     /locus_tag="MJF80_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBZ0281817.1"
                     /translation="NNYNLKDMSITYVFFSNLLSMYNKKTINKKLAIVFLISIVVQAI
                     IQYLVLIFFMYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIEN
                     GNLKYKLEVIGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPL
                     TSILSYVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITK
                     TDIRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYS
                     MPNTRVYIDMFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSI
                     AQNIIELENAHMEIIIDADLFKVMLTFRRVN"
BASE COUNT         4250 a         1210 c         1961 g         3265 t
ORIGIN      
        1 aataaaaatc aaaaaaaatt tatcaatgaa agttagctaa aatcctggaa gagagcatta
       61 tttttgggaa aacattttct tttgtttcac aaaaaaagcc actttttggc acacaacatg
      121 ctttatataa agtcaagaaa aactaaggta taagacaaag gcaattagga ggtgtaaata
      181 tggagtttat aaattttgta agtgataaag ttatagacct taaaatggat tgtaaaacga
      241 aagaagattt ctttaaagaa atacataata aagtttttga tttaggcttt gtaaaagaag
      301 aatttggaga aaaaatatta gcaagagaaa atgtatttcc aacaggttta aatttaggtg
      361 attatggagt agcaatacca catacagatg cagaatatat taaagagcaa tttatatcag
      421 tctgtacttt taatgaacca gtagtattta gttctatgga agaccaagat gaaaaagtac
      481 ctgtaaattt ggctttcgta ttaggactta atcaaccaca cagtcaatta tctgtattaa
      541 cagaattaat ggcaattatg caaaataaag agttagttga taagttgatg agttctactg
      601 ataaagaaga agttttaaag acactaaagt ctttataaaa ataaaaaagt actaaaaata
      661 aaaaatataa aaataaaaat tattaggagg aaataaaaat gaaaaaaata ttagtagcat
      721 gtggagcagg aatagcaaca tcaacagtag tttgtgacaa ggtagagaga ttagttaagg
      781 aaaataatat acaagcagaa gttatacaat gcaaaatagc tgaatgtgca acaaagcaag
      841 aaggagcaga tttaatagta tcaacaacta tactaccaac aacttataat ataccagcaa
      901 taaaagcaac agcatatata actggagtaa atacagcagc attagataaa aaaatattag
      961 atgctttaaa ataatcctat catagataca ttgaaataat attttataaa acagaaaaag
     1021 gaggatttat aaatggataa attattagca gtagtacaat acattttaaa tatgggaccg
     1081 acagtaatac taccaataac aattgtagta ctaggaatga tatttagaat taagtttaca
     1141 aaagctttaa agtcaggtct tacaattggt ataggatttg tgggtataaa cttagttgta
     1201 aacctattaa catcaacact agggccagcg gctcaaggaa tggttgaaag attcggactt
     1261 aatttaacag taatagatgt tggttggcca acaacagcat cagcaacatg ggcttcacca
     1321 gttgcaccaa tattaatacc agtttgttta ttagtaaact tactattatt atacttcaat
     1381 aaaacaaaga tattaaacgt tgatatatgg aattattggc actttatagt agcaggtgca
     1441 gttggatata tagtaacagg aagttatgtt tgggctatat tctgtgcaat actaatggag
     1501 ttattagtac tgtttattgc agaaaagaca gcaaaaaatg ttcaggagtt ttatgattta
     1561 gaaggtatag cattaccaac tggttcaaca gcttcatttg ctccacttgg atatttagta
     1621 ggtaaatttg ttgaaaaaat accaggaata aataaaatac aagcagaccc agactctata
     1681 caaaaaagat ttggtatatt tggagaacca atgatgatgg ggattatact aggtataata
     1741 cttggagcac ttgcgggata tgatgtagca ggaatattca atgtaggtat atcaatgggt
     1801 gcagtaatgt tacttatgcc tagaatggtt agaatattaa tggaaggttt aattccaatt
     1861 tcagaatcaa taagagattt cttacaagca aaatatggag acagagaact ttatataggt
     1921 ctggatgcag cagtagcaac tggacatcca gcagttatat caacagctct tatacttgtt
     1981 cctataactt tgttcttagc agttatacta ccaggaaaca aagttttacc atttggagac
     2041 ttagcgacaa taccatttta tatggcattt atagtgtgtt ttagaaaagg taatatagtt
     2101 caatcagtta taacaggaac aatagtaata gctatatcac tttatatggc tacaaacttt
     2161 gcagatgttc atacacaatt attacaacaa gcaaatatga tgccagaagg aacaacaatg
     2221 gttacaagta tagatacagg tggaaacttg ctaaaatggg gaatattgaa attatctcaa
     2281 ttagtttctt catttggaat attctagtat gtagttaata gagattactc ttttttagta
     2341 aagtatgcga aaatttaaca tttagcagct actaaaaaag agtaatattt tttataaatt
     2401 tttaaaaata caaaatacaa gaggaggaga tttatcgtga aagcagcagt attacatggc
     2461 acaaatgata tgagatttga agatatagaa ataaaaccat gtgaaagtga tgaagtaaaa
     2521 ataaaagtta tggcagcagg aatatgtggg tcagatcctc caagagtatt aaaacattgg
     2581 aaatatccag ttccagctat accaggacat gagttttctg gagtaattgc tgaagttggt
     2641 aaggatgtaa aaaatgtaaa agttggagat agagtagttg caattccttt tataccttgt
     2701 aatgaatgtg aatattgtaa aagaggatta ttttcattgt gtgataacca tggaatgtta
     2761 ggagctaaaa gttttggagc ttttgcagag tatgtaaata taaaagctac aaatgtactt
     2821 ccaataggag atatgaactt tgaagatgct gcaatgatag agccactagc agttgctatg
     2881 catggagtac ttaatatagg tgttcaagtt ggagacactg tagctgtaat gggtagtgga
     2941 acaatggggc agttggtaat acaaggactt aaaatagcag gggcaggaac aattatagca
     3001 gttgatatat ctgataataa attaagagaa tcaaaggaat taggggctga tataataatt
     3061 aatgctaaag atactaaccc agtagaaaaa ataaaagaac ttactggtgg aaaaggtgtt
     3121 gacatagctc ttgaatgtgc aggttcaaaa ataactcaag aacaatgttt acttataact
     3181 aaaaagaaaa gtaagatagg atttttaggg atagcatatt cagatataac tttatcagaa
     3241 gaagcatttg aaaatatatt tagaaaagaa ctagaattaa aaggtttttg gaactcttat
     3301 tctgcaccat ttccaggaca agaatggact aagggaatca atctagttaa tgaaggaaag
     3361 ataaaattaa aagaaatggt atctcataga ttctcacttg aagatactta caaagcattt
     3421 gaaatgataa gagatagaaa agaagaattt aacaaaatat taattttacc acaaggggtt
     3481 gaaaaataat gaaagcagta gttaaaacta aaccaggcta tgataatgta gaagttttag
     3541 aagtagaaga accaaaagct acaggagaca aggttaaaat aaaagtagaa tatagtggta
     3601 tatgtggttc agatatacat tcatttaaag gtgaatatgc aaatataaaa gccccagtaa
     3661 cattaggtca tgaattttct ggaattgttg tagaagtagg agaagatgtt aaaaatataa
     3721 aagtaggaga tagagttaca tcagagacta cttttgcaac ttgtgagaaa tgtatatatt
     3781 gtgaaactaa agattataac ttatgcccaa ctagaaaagg tattggtact caagtaaatg
     3841 gaagttttgc tgagtatgta ttatcaagag aagaaagcat acatgtactt cctgaaaatg
     3901 tatctttatt atcagcagcc cttacagagc cactagcttg ttgtgttcat gcagctcttg
     3961 aaaagactac tatagaaagt tctgatacag ttttaataat aggaccagga ccaattggtt
     4021 tattattagc tcaagttgtt aaatctcaag gtactacagt tataatgtct ggtgttacaa
     4081 aagataagga aagattagaa atagctaaaa atcttggagt agatagaact gtaaatgtaa
     4141 tggaagaaag tttggcagat gtagtgaatg aaatgactga tggaaatgga gtaaacaaag
     4201 gatttgattg ttctgggttt atgccagcag ttaacgaagc cttaagactg cttagaaaaa
     4261 aaggtacttt cgtacaagtt ggaatctttg ctaaaaaatt aaatgaaatg gaccaagaag
     4321 caataatcca aagagagtta caatatatag gaagcaggtc tcaaaagcca agttcttgga
     4381 ttacagcatt agatttaatg gcaaatggga aagtaaatac agaagcttta gttactagaa
     4441 ctgttggttt agacgaattt aaagaaggta ttacagcttt aatgaacggg gaagaaataa
     4501 aagtagcagt aaaatcttaa aaaaacattt gtattatttt aaaaaaaatt gtaaaatcta
     4561 ctgtaggata aaaaaatgta aggtcttaaa aaataacagg aggaagaaag tatgagcact
     4621 cttagagatc attctaataa tattagaaaa aacataataa aaatggttgc agaagctaaa
     4681 agcggacatc caggtggttc attatcaata gctgatatgt tgacagtatt atattttgat
     4741 aagatgaacg tatgtgcaga aaatccaaaa atggctgata gagatagatt tgttctatca
     4801 aaaggtcatg cagcaccagc tttatatgct acattagcag aaaaaggatt tttcccagaa
     4861 gaagaattaa taacactaag aaaatttggt tctaagttac aaggtcaccc agacatgaaa
     4921 aaagtagcag gaatcgatat gtctactggt tctcttggtc aagggctatc tgcagcaaat
     4981 ggtatggcac ttgcaggaaa attagataac aaagactata cagtatatac tatattaggt
     5041 gatggagaaa tccaagaagg tcaaatatgg gaagctgcaa tgacagcatc acattacaaa
     5101 ttagataatt taattgcatt tgttgactta aatggattac aaatagatgg ttctaatgaa
     5161 gaagtaatga atgtaaatcc agtagatgac aaattcaaat cttttggatg gaatgtaata
     5221 gtaataaatg gacattcttt tgaagaaata ggaaatgcag ttgatgaagc taaaaaagtt
     5281 acaggaaaac caactgtaat aatagctaaa acagtaaaag gtaaaggtgt ttcatttatg
     5341 gaaaataatg ctgcatggca tggaacagct ccaaatgcag aacaagcaga gcaagcattg
     5401 aaagatttag aaggaggtct ataggaatgg gaatagcaac tagagaagct tacggacaag
     5461 tgttaaaaga acttgcagaa aataaagata tagtagtatt agatgcagat ttaggaaaag
     5521 ctacaaaaag tatatcattt aaagaagttg ccccagatag attttttgac atggggatag
     5581 cagaaggtga catgattggt acagcagcag gtcttgctac ttgtggaaaa ataccttttg
     5641 cgagtacttt tgcaatattt gcagcaggaa gaggctatga gcaaataaga aactctgtag
     5701 catatccaaa tttaaatgta aaaatagcag ctactcatgc aggtgtaaca gtaggtgaag
     5761 atggtggtag ccatcaagct atagaggata tatctctaat gagaggtata ccaaatatgg
     5821 ttgtattaaa tccagcagat gctttagaag caagacaagc tattcttgca tctattgatt
     5881 ataatggacc tgtttatata agattaggta gagctgcaac tcctgatgta aacagtgaaa
     5941 attataaatt tgaaatagga aaaggaactg ttttaagaga aggttctgac ataacagtaa
     6001 tagctacagg aataatggta gcaaaagcct tagaagcagc agaagaactt gctaaagaag
     6061 gtataaatgt agaagttgtt aatatatcta caataaaacc attagatgaa gcattaataa
     6121 aagaaactgc taagaaaact ggaaaagtag ttacagcaga ggagcacagt ataataggtg
     6181 gtttaggttc agctgtatgt gaagctttag cagaaactaa agatgtagta gttagaagaa
     6241 ttggtgttaa agatgtattt ggtcaatcag gtacaccagc tgatttatta aaacactatg
     6301 gtttaactac agaagatata gtaaaaaata taaaagagtt attatagata taaatatatc
     6361 taaataacaa attgatttga cataagtcaa ttaaagctgt cttaaaaata gaaagaaatt
     6421 tctacaatgg gacagcttta tttttacaat actttatttt tatgataatt ataaaatagt
     6481 ctgaaagtaa tcaaaaaaat tataaaaaga ggtgctattt aatgaaaata tctattggat
     6541 gtgaccatgg tggttatgaa ttaaaggaat atataaagga aaatttaata aataaaggta
     6601 tagaagttga agatgtgggt acaaattcaa aagaaagtgt agattttcca atatatgcaa
     6661 aaaaggtaag tcaaaatgtt caaagtaaaa aatctgattt aggcatactt tgttgtggaa
     6721 caggaatagg tatgtctatt gcagcaaata agtttaaagg aatcagagca gctgttgttt
     6781 ctgactgttt ttcagctaag gcaactagag aacacaataa tacaaatgta ctttgtttag
     6841 gagaaagagt tattgggaaa ggattagcaa tgagaattgt tgaagaatgg ttaaattcaa
     6901 gttataatgg agaaagacat gcaagaagat tgcaaatgat tgatgatata gaaaaagaaa
     6961 atttataaat atataattta taacaagggg gagcgttatg tctattattt gtgcttcaat
     7021 aatgtgtgca gaccaaatga agttaaaaga ggaattagaa gctcttgaag aagcaggtgt
     7081 aaaattactt cattgtgatg ttatggatgg aatatttgtt aaaaacttgg ctatgggacc
     7141 agagttatta aaatcaataa gtgaaaatac aacaatacct ttagacatac acttagccac
     7201 agaaactcca gataaatata ttgatatgat gtcttatata aagccaaaat atatttcttt
     7261 tcatgtagaa tcaagtacaa atgtaaaagc tgatatacaa aagttaagaa attatggtat
     7321 agggccagtg ttagcaataa gccctcaaac aagtgtggat aaaatagaag aatatataag
     7381 tttagttgag ggtatcttag taatgacagt aaatccaggg tttgcaggtc aaaagtttaa
     7441 tctttctgta ttggataagt tggataaact tacagagata ctaaaagatt atgataatcc
     7501 tccatttata gaggttgatg gaaatataaa taaggatact attaaactta tgaatggtaa
     7561 aaaagtagat atatatgttg ttggtacttc agctttattt aatgataagc ctccaatttc
     7621 atataaagat aaaatagaag aactaaaaga aagtataaag taaccatgta ataaaattat
     7681 aatatgtttc tgtttaatag taaaaattta ggtggaaaca agaaatttta taatatttaa
     7741 taaaaatttt ggttgagtaa ggagaatttt ttatgggaga cctatataaa ctacaaaatg
     7801 gtactgatat tagaggaata gcgtatgaaa atgattctaa agaagttaac ctaactgtag
     7861 aagaagtaaa aaaaatagct aaagcgtttc atatttggtt aaaagaaaag actaagaaag
     7921 ataaagtaac tgttgccata ggaactgatt ctaggataac aggaagccaa tttagaagta
     7981 ctgttattga aactttaact aatgatgatt gtaatgtaat tgattgtgag attgccacaa
     8041 ctccagctat gtttatgaca actattatgg atggatacaa ttctgatggg gcgattatga
     8101 ttacagcaag tcacttacct tattactata atggtttaaa gtttttcaca gaaagtggag
     8161 gtttagaaaa aactgatata aaagagatgt tggatatagc tgttaaaaat gatagtgtag
     8221 actatgagga agtaaataaa aaaggtgaag ttatagtaaa aaatttaata gaagattatt
     8281 ctaatttatt aatagataaa attagaaaag gtgtaaattc aagtaaaaat tatgaaaagc
     8341 ctttttctgg gcttaagata ttggttgatg caggaaatgg ggctggtgga ttctttgctg
     8401 aaaaagtttt acacatactg ggagcagata cgacaggtag tcagtttttg aacccagatg
     8461 gtatgttccc aaatcatata cctaatcccg aaaacaaaga agcaatggaa agcatatgta
     8521 aagctgtgtt agataataag agcgacctag gaataatctt tgatacagat gtagacagag
     8581 ctgctattgt aggtaaaaat ggcaaaccaa taaataaaaa tgctcttata gctgttatat
     8641 catcaatagt attagaagaa catccaaaaa cagctatagt aacagattcg attacttctg
     8701 aaggacttgc taaatttata aatgaattac aaggaagaca tcatagattt aaaagaggat
     8761 ataaaaatgt aataaatgaa gctataagat taaatagtga aggtgaagag tgccatttag
     8821 ctatagaaac gtctggacat gctgctttaa aagaaaatta tttcttagat gatggggcat
     8881 atttaatagc taaaatttta ataaaagtag caaagctatc cttagaagga aagactatag
     8941 aagaattgat agaaaacctt gaagaagctc aagaagagaa agaagttaga ataggcatta
     9001 ataaagaaga ttttagacca tatgcagagg atatattaaa agatttagat tctcatgtca
     9061 agagtattga tggatggtct gtagcaccaa ataattttga aggcataaga gttaattgtg
     9121 atgaatcaaa tggagatgga tggtttttac ttagaatatc actacatgaa ccattattag
     9181 ctttaaatat tgaatcaaat aaaattggcg gagcagaaca aatttattct aagttaaaac
     9241 ttttcttaga aaaatatgat ttaaaaggtc tttagggtaa atagaaagaa tatatttata
     9301 ttgaagaata aatgtaaatt tgtacctaag attacaaata ttacaaaatt gtaatcttag
     9361 gtaacagaga tgtaactgat tatggaaaca ctatgtgata ttatagatac agagagatga
     9421 aatatgtgat gaattataga gaaatataat ttaaaatgta ttaatatcac accaaaaagg
     9481 tataatcaaa aaatatgtaa atataaaccc caacatttag aaaaagacct ctaaggaggt
     9541 ttttttcttg tttaaataac ttaaaatata tttttatatc aatatatcaa ttaactcttc
     9601 taaaagtaag cattacctta aataaatcag catctataat tatttccatg tgtgcatttt
     9661 ctaattcaat tatattttga gcaattgata aaccaagacc agagccatct gtttttctag
     9721 atatatcacc ccttctaaat ctttctatta actcttctgg ctttagattt agtttatcat
     9781 ttgaaatatt tttaaacgtt aatattatat tttcttcctt tataaacata tctatataga
     9841 ccctagtgtt aggcatagag tattttaata tattgcaaat tagattctca aaaactctcc
     9901 aagttttttt accatctatc atggtaaaaa cagcatattc aggtgtttct attataaaat
     9961 ctaaactgct attttgaatt ttgtcttcaa attctacaat actttgaatt aaaagttctc
    10021 ttatatcggt ttttgttata ttaaattcta catctccact acttagctta gatgcttcaa
    10081 atatgtcatc tattaataat tttaatcttt gtgatttttt atctaaaata tttatatagt
    10141 catttatagt ctctctgtca aagtcattat tttttaacat atcaacatag cttaatatag
    10201 atgtaagtgg agtttttaag tcatgagata cgtttgtgat aagctctgcc ttcatccttt
    10261 cattttttat attagcatct acagcagttt caagaccgcc tgttatatta tttatacttt
    10321 tggctaaagt agatatttca tcatctccta taacttctaa tttatacttt aaattgccat
    10381 tttctatatt ttttatattt tttgatatgt tatttatgta attaaactta acagataaaa
    10441 agttgtatat ataaaaatga aaaactgtgt ttacaagtgt aaataaaatt actatagcta
    10501 ttgcctcagc ataatatata tacataaaga atatcaaaac aagatattgg ataatagctt
    10561 gtacaactat agatattaaa aatactatag ctaatttttt atttatagtt tttttattat
    10621 acatactaag taagtttgaa aagaatacat atgttataga catatctttt agattatagt
    10681 tattat
//
LOCUS       DAGSWI010000136        10511 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.137, whole genome shotgun sequence.
ACCESSION   DAGSWI010000136 DAGSWI010000000
VERSION     DAGSWI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.137"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..447)
                     /locus_tag="MJF80_003180"
     CDS             complement(<1..447)
                     /locus_tag="MJF80_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein complex assembly protein"
                     /protein_id="HBZ0281818.1"
                     /translation="MEVSIILVKDIKQDIESMPRKILDISLIEDNKSDLTFFNIEEED
                     GDETLKALKQSYVTTNYKEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKN
                     YITSAINMVNKALEINPKDIDILNLRGLLKLLKCDFAKSFESFYTAL"
     gene            complement(578..1171)
                     /locus_tag="MJF80_003181"
     CDS             complement(578..1171)
                     /locus_tag="MJF80_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBZ0281819.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(1236..2690)
                     /locus_tag="MJF80_003182"
     CDS             complement(1236..2690)
                     /locus_tag="MJF80_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBZ0281820.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(2805..4196)
                     /locus_tag="MJF80_003183"
     CDS             complement(2805..4196)
                     /locus_tag="MJF80_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBZ0281821.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(4362..4610)
                     /locus_tag="MJF80_003184"
     CDS             complement(4362..4610)
                     /locus_tag="MJF80_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281822.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            4876..7005
                     /locus_tag="MJF80_003185"
     CDS             4876..7005
                     /locus_tag="MJF80_003185"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBZ0281823.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLARPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            complement(7232..7831)
                     /locus_tag="MJF80_003186"
     CDS             complement(7232..7831)
                     /locus_tag="MJF80_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBZ0281824.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(7849..>10511)
                     /locus_tag="MJF80_003187"
     CDS             complement(7849..>10511)
                     /locus_tag="MJF80_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBZ0281825.1"
                     /translation="SDAIDIINIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTW
                     EWSKFLEEANYDLSNNINIKKEYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTI
                     WENKEGFTYEYRPGQYELTKTIRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEA
                     RINKKRLLISTDTKELQIQLINKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAY
                     IDDYESQNPTKGEILSLIFLKRLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPN
                     MCRPKKCKKDCLYKNRIEELKEEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEK
                     GYDFFSSIINSKSLRYLLQEIYPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGR
                     EDKQKIARSINFIIEEIDSILKFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGID
                     TEISYRAYSEKIKLSCEKIIKNLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLED
                     IKIIFEIFLEYDEKDDYARIVEIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLS
                     ATLSLSESMSYFKNTLGIDRVKNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEM
                     SKIISNISKITEGHTLALFNSKDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNR
                     KCVVLGSKGCFEGVDIPGDGLVCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQM
                     TIKVKQAMGRILRSKYDYGCFVIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIR
                     RHLNKSRSLILKSVIFDTIKALNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHI
                     DIIKKDMKVKYFDRNYLINLDIFMREEDKN"
BASE COUNT         3399 a         1733 c         1090 g         4289 t
ORIGIN      
        1 taaagcagta taaaaactct caaatgattt agcaaaatca cattttaata attttaaaag
       61 acctctaaga tttagtatat caatgtcttt aggatttatc tccagagctt tattaaccat
      121 attaatggca ctagttatat aatttttata tgctaaatcc aatgcattgt tatatctttt
      181 atatgattct aattttaact ccttagacgt tgtatctgac tttgaattta aagctccttg
      241 caagtttaat agagtttctt ctttataatt tgtagtcaca taactctgtt ttaatgcttt
      301 taatgtttca tcaccgtctt cttcttctat attaaaaaaa gtcaaatcac ttttattatc
      361 ttctatcaaa gatatatcta aaatttttct aggcatgctt tctatgtctt gttttatatc
      421 tttaactaaa ataatactca cctccactct aaaatatata tttaagataa catatttaat
      481 atgtcaagtc aaaactatac atatttttcc accattctca taataaaaaa ggtaatcctc
      541 ttcttttcat aaaaaggtta cctctttttc aatactatta acttttaatg ttgaattttt
      601 ttacttttct atagagtgta gctataccta tatttaatgc ctcagctact tttttcatac
      661 cttctgtatt acttccatat ttctttatgg cattttctat agcttccctc tcaaggtcat
      721 ctatagtctt aacttcttgt ttataaagtt catctagttt aaaagatatt aaatcagaca
      781 tactccttac aaaagcagtt aactccttat cttttgaaag tatcttttct ttttgttctt
      841 cattaaacgc agctataccc aagattccaa gtatatgctc tcccatttta acgggagtac
      901 atatatcagc taactcttta caattttctc tatttgcaca tttattacag ttctcttcta
      961 tttctttatt tataacatag gattttccat cttttaaaac aagagagtac actgacttct
     1021 ctgatattgt ctctcctatt ttacctctga atattcctgt acctgctatc ctagttaaat
     1081 cttttgtaac taaagtaaca tctacatcca agacattaga aatattctcg caaatacttt
     1141 gaacgtaatt cgttatatta tttaaattca aagtaatccc tcctatttag atttatattt
     1201 tttattactc tacataaatc ctattcttga gaactttaag cttgcggttg tggttgtgtc
     1261 tcattaggct tattatcagt tggtggtgtt tctggttttt gttgattatt tggattttga
     1321 ttattcttat tttcgtctgt atttggtttt tcctctggtt ttggttccat agtcccaact
     1381 aatataatac cttccttctt aggataatat gaattagcta ttttttcagt tttaattttt
     1441 ttaccatttg catcattaaa tgttctatat gttgatacag aatatgcatt tcttccactt
     1501 ttgtctactt tttctttacc tttaggcatt gtagggtcat taactctttt cattgtagtt
     1561 tgtgaaacgc catctaattt tgttgatatt gttatatttt gtttatcttc tgcacttcca
     1621 tatatattgc aaacaatttg atttcctgaa acgtaatttt ttatgtaaac tggatgcttt
     1681 agattatttt taaataagaa atctattcca ctatctgcaa cagttgcatc tcttcctttt
     1741 ggaacataac ttgatggtat agtatgattt tttacattaa caatttctaa acctgcgtat
     1801 aacacagaat tgtataacgt agatgaaact tgacaaactc ctccaccata atcttcttcc
     1861 atctcacctt ccattattac aggtgcactt ttatatccat tttccttact tcttttccct
     1921 gtatgtttat taaatgagaa agtttctcct ggcataagta atacattact tatattatct
     1981 gttgctaatt ttatgtttat acttctattt atttgagaag ttttaaatgt tgttgaatac
     2041 gtacctaaat ttgtatctat attagcaagt tgctcttttt ttacttttgc tggagtagct
     2101 tttactacta actcattttt atagttacct tttttagttt gattatcaaa attctccata
     2161 ctcttagaaa tatccatttt aagaccatca gaatctggta taaccttgac tttttcacca
     2221 cttatatcaa gtgtagcatc ttttacgtct acatctattt ctttagctat cttttccatt
     2281 tctgctttta atttattttt atcataattt atcgcaacta ctaagttgct ttttttgcca
     2341 aaatttgctg aaatagtttt tgatagattg ccaaagaaag cactctttct atttaaatta
     2401 tatgcgtttt ctactgtttt atccaggtca gtagataaat ttaaatcttt gctctttact
     2461 ttccaacttt tatcattgta attaaaatca acattctcta atttgtattt tttagctaat
     2521 tcctgttttg cttgcgattt agttaatttt ccaatattta ctccattaac atatgtgttt
     2581 tttgctattt tctccccttt aaattgcatg cacattatgc ctataaaaat tattactact
     2641 aatgctacag ctccaatagc tataactagc tttttgttca cattactcat cccatacttc
     2701 tccttacaat aaaattattt atatatccct attattatat tactacataa tgcgccttaa
     2761 aaccccaaaa actttttaat tatatattaa tttaatatta ttaattaata ttcatttagt
     2821 ccattttctg atatggcata tattttttca ttgtaaatat taatagcata aacttctttg
     2881 cctctaaata agcctttatt tactacagca taataataaa tatttttata ttttccaaac
     2941 aattcttcta aactcttttt actttttaca tatctatctg ctttctctat atctaatatc
     3001 tcaatctttt tatacttatt ttctttttca tcaagagtat tagttaacac aaacttagct
     3061 ttatcttttg taataccaaa ttgataactt ccatcttcta atattctaaa gtatccatca
     3121 ccataatcta ctttgtaatc ttttttacca tctttatatg aatataaatc tttagttttt
     3181 gcacttacca aaacttcatt tattaattta tcatactcat atacttcagc ataatagtat
     3241 ttattatttt ttatcatttg tggaagtgaa tttatagata gatttgcctt gtctttgcca
     3301 ctttttttag ctattacatt tataatgtca tcagatgtta aagtatacaa tgataaattc
     3361 tttttatatt ttttttctaa actctgtacc ttttcatcat ctgaataatc gtcttttaaa
     3421 tactcaatat attgtaatgc tctttcataa tctccagaag tatttgcctc ttcagctcta
     3481 cttatatagt aatcatacat ttcttcaata cattgttctt tcccattttg agccatactg
     3541 taatactttt catcttcttt taatacttta ttatactttt caatagcttc ataatactta
     3601 tatgcttttt ggtcttttac tgctgagtca taaacatctc tagactcttt gattgtttct
     3661 atattctgtt tgtatacatc taggttacta cccataccat ttaatattga aacagtactt
     3721 atgtagttaa tggcaatatc ataagtagta ttttcttctt tatacatttc tctaactcta
     3781 tcagcttgag ctaataagtc atccacatct atacttattt tgtttaattc tttcacggta
     3841 tttattaatc caataaaact ttcttgactt atgtctccat ctaaatattt atcaccacta
     3901 tttaatagca atttatttat ttttttagaa acagacttat taaacctttc catttttgaa
     3961 tcagaaaact ttttttctat acttgtgtag taatcaacag ctatagaata ctgtcttttc
     4021 tctataatct ttaacaactc attagcttca tttcctacac taaactcgtt actacttaag
     4081 aaaaatacta ttatcaaagc aattacacct aatgttgata atgaaatcgc aactatagat
     4141 ttcctttttt catcaataaa atctacagcc ttctcaaata aatctctaaa attcattata
     4201 gttccctctc catttaataa tctttatatt ttatggtaaa ctaaatttct tattggttat
     4261 tatatcataa ttcttgaaaa aaaataaaaa cacaagttaa atcgtgtttt tattttacct
     4321 atcaatcatt attttataca taaatatttc ctttacataa attatgatat ctttcttttt
     4381 atatccctag aatgactctt ttgtttttta ttattactga cagttttcgt tcttgtcttg
     4441 tgtatagctt tgctatcaaa tatttttcta gcattgcata aaacaattcc aattgttaaa
     4501 caaactgtaa gcacacttcc catcaaaaag taataatcca atcctatatg tactcccata
     4561 actgaattta ttaacactaa tgcaaaagtc attgcaaaac atatcatcat ggagataact
     4621 aaaattctta gaattttttt caatccactc acctcgtata atcactaaaa ttgacactac
     4681 cccttatcca ttactatacc aaattagtaa aaaaatagca attactattg tcatttatat
     4741 taggaaaata ttaaatcttt ttaataaata tctttttttg acataaggat tacttaactt
     4801 ttaataaaat agtaacacta atatgtatta attaatagaa taaaataaat gataatttat
     4861 tgaggggtga tttacatgcc aaatttgcca agtttagggt caaaggctcc tgattttaaa
     4921 gccaatacaa caaatggtcc tattagactc tctgactata agggtaattg gattgtttta
     4981 ttttcacatc ctggtgattt tacaccagtt tgtactacag aatttttatg ttttgctaaa
     5041 tattatgacg aatttaaaaa aagaaataca gaactaattg gtctaagtgt tgatagtaac
     5101 agttcacatt tagcttggat gtataatatt tctttactta caggtgtaga aattccattt
     5161 cctattatag aagatagaga tatgagaatt gccaagttat acggcatgat atcaaaacca
     5221 atgagtgata catcaactgt tcgctctgta tttattatag ataataatca aattctaaga
     5281 acgattcttt attatccact aactacagga agaaatattc cagaaatact tagaatagta
     5341 gatgcacttc agactagtga tagagataat atagttactc ctgcaaactg gtttcctgga
     5401 atgccagtga ttttacctta tcctaaaaac tataaggaat taaaaaatag agttaacagt
     5461 tgtaataaga aatattcatg tatggactgg tacttatgtt ttgtaccaga taattataat
     5521 gatgaagaag tgagcaagaa aattgataat acttgtagct ggaaaaaaga acatactaaa
     5581 aacattgaaa atgaatgtaa ctgtgaacat gaacatcatg actacctgaa caaagctctt
     5641 gactgtaaac aagaacacaa gactgatatt aaagatgatt gcaaccatga gaaaaaacat
     5701 actaaaaata ctaacaaagt tcacaactcc aaacaagata agtttaaaga taagtcttgt
     5761 gatgaaatga attttaacta tgataaagat gaatcttgtg acaaaataaa ttctagctat
     5821 aacaaagaag atagtagtta tgaagatttc tataaacata attataaaaa ctacgattat
     5881 actagtgaaa aaaatactaa aaaaatagct atgaagactt taaaagattc aaaaaaacta
     5941 gctagaccac aaataacaga cccatacaat ccaatagttg aaaatgcaaa ctgtccagat
     6001 ataaatccaa ttgtagcaga atatgttctt ggaaatccaa ctaatgtaga tgctcaacta
     6061 ttagatgcag ttatatttgc ttttgctgag atagaccaat ctggaaattt gtttattcct
     6121 tatcctagat ttttaaatca attacttgct cttaaaggtg aaaaacctag cttaaaagta
     6181 attgtagcta ttggaggttg gggagctgaa ggattctctg atgcagcatt aacacctaca
     6241 tccagatata attttgcaag acaggtcaat cagatgataa atgaatatgc tttagatgga
     6301 atagatatag actgggaata tcctggaagt agtgcatctg gaataacatc aagacctcaa
     6361 gatagagaaa attttacact cttactaact gccataagag atgttatagg agatgataaa
     6421 tggcttagtg tagctggaac aggagataga ggatatataa attcaagtgc tgaaatagat
     6481 aaaatagctc ctataataga ttattttaat cttatgagtt atgattttac agcaggtgaa
     6541 acaggcccaa atggtagaaa acatcaagca aatctttttg attcagactt atctttgcca
     6601 ggatatagtg ttgatgcaat ggtgagaaat cttgagaatg ctggaatgcc ttctgaaaaa
     6661 atccttcttg gtataccatt ttatggaaga ttaggtgcta ctataacaag aacttatgat
     6721 gagcttagaa gggattatat aaataaaaat ggatatgaat atagatttga taatactgct
     6781 caagttccgt atttagttaa ggatggagat tttgcaatgt catatgatga tgctttatca
     6841 atattcttaa aaactcaata tgttcttaga aattgtctag gtggtgtatt ctcatggaca
     6901 tcaacttatg accaagcaaa tatactggct agaaccatgt ctattggtat aaatgaccct
     6961 gaagtattaa aagaagaact tgaaggtatt tatgggcaat tctaatagaa tgtagtaaaa
     7021 ttttatagtt atataaaaag cagattttat tatattaaat aagttgtatc taattaatac
     7081 tattattaat tgtttataaa ttagatactt attttttcat aatacaatct gcttttttaa
     7141 tcaaaattta tttaattaca agttgtttaa ttataaagta ggaatttaga tatactctta
     7201 taccatgtaa ttatgtataa atattttttt attatacacc aatagctttt ttagctaatt
     7261 tatcagcttc ctcattatac ttatttccag aatgagcttt aacctttgta aattttacat
     7321 taagcttatt cttaatactg tcatagaaat ttttatatgc tatagttcct tctttatttg
     7381 ttttccaaac tccagtacac cacttttcaa tcccttcata gtcaaagtat aaaatcaagt
     7441 tttgaacatt attgtcaata cagtactgca ttgcaatttt agaagcctct atctctccag
     7501 ctacatttct catacttact aaagttttct cttttccttt ttcactataa gtttttataa
     7561 cttcattatt ttttaaaatc acaacacccg aaccatacat ttttactgaa tgttcataac
     7621 ttccatctac atatgcttct ataaactcct tatcttcttg aaaactcaat ttttctgacc
     7681 catcaatata ttctttagct tcttgaaaag tagaaaaact cttatattca gctcctgaga
     7741 atccatttac ttgttttttg cactcatccc atgtattata tacacctata tttttacctt
     7801 ttttcacagc ataaaacttt tgttttgcca aatatatcac tcccactctt aatttttatc
     7861 ttcttctctc ataaatatat ctaaatttat aagatagttt ctatcaaaat attttacttt
     7921 catatctttt tttataatat caatatggta cacttctcct tttacagctc tttgtcttat
     7981 attttcattt atatcttttt ttataatatc cttatcaaca tcattgttat ccattttagc
     8041 atcaacattc aacgctttta tagtatcaaa tataacagat tttagtatta aacttctaga
     8101 tttatttaaa tgtcgcctta tataagtata aaactcattg ctattaacct ttgaaatttt
     8161 acaatcgtgt aaatctttct ctaatctctt taacacactt atattagttc ctacatcaaa
     8221 aatcacaaaa catccatagt catatttgct tcttaaaatt ctccccatag cttgctttac
     8281 ctttatggtc atttgaggat aatttatagt atagtagtct ataccatact tcttcattat
     8341 agtaaaatac aaagggtctt taggatttag attcggaagc ttgtctaaag ttacacaaac
     8401 caaaccatcc cctggaatgt ctacgccttc aaaacatcct tttgagccaa gtactacaca
     8461 ttttctattt aaatcgttaa ggtgtctaat gcccttttta tcagcataaa tttctaagtt
     8521 aaaactatgt aagtattttt tcaatatttc ataagttttt tcttgtctat ctttactatt
     8581 aaaaagagct aatgtatgac cttctgtaat ctttgatata ttagatataa ttttactcat
     8641 ctcatttgga aattcagaat ttctgtactc acaaatatca gaaaatccaa ctacagagac
     8701 tctattttta taatcaaaaa ttggttctat aatcttttcc acattcttaa ctctgtctat
     8761 tcctaatgta tttttaaaat aagacatact ttcagataaa ctcaaagttg ctgataaaaa
     8821 tattcctttt tctaattgag ataaaatatt ttcttcaaat aaatctgcta tttttaatgg
     8881 tactactcga aattcaaagt cattataatt cttatcaatt tcaactatcc ttgcataatc
     8941 atccttttca tcatactcta aaaagatttc aaatataatc tttatatcct ctaaatctct
     9001 tgtctttgcc tttccaaatt tatatatatc tgcttcttta tctatactat catcatcaat
     9061 attcctataa attattatta ttattgaaac taaatttttt attattttct cacaactcaa
     9121 ctttatcttt tcactatagg ctctataact tatttcagta tctataccat ctttttttag
     9181 ttttcccaca atttcatcta tttgcaaatt cagttcccat cttaaattat aattacttac
     9241 attattatat tcattacaat taccaaactt taatattgaa tctatctctt caataatgaa
     9301 atttatactt ctagcaattt tttgtttatc ttctcttcca atttttaata cattataaaa
     9361 tttatcaaaa gcctttattt ttctcatatt tctactatat tttttatata taaagctact
     9421 attttgaata aactcgtaag gataaatttc ttgtaataag taccttaaag actttgaatt
     9481 tataatactt gaaaagaaat catatccttt ttcagtcaaa ttatgtgctt catctactat
     9541 tatattttct aaaggttttt catccttata cggccattta gctaatagtg aatgatttac
     9601 tactgttata tgttcttctt ttaattcttc tattctgttt ttatataaac aatctttttt
     9661 gcatttttta ggtctgcaca tatttgggtc acaacttaca tttcttaagt gagtacttat
     9721 ttccttgaag ttgtcaaaca ctaaataatt tatttcttct atatctccat acttgccacc
     9781 ttcaactaat ctcttcaaaa atattaaaga taaaatttct cctttagtag ggttttgaga
     9841 ctcataatca tctatatatg cttccagtct atctatacaa atatagttat tttttccctt
     9901 tatatatccg taacttactt ttccatttag acccaaagaa tctaacacat ttggaatatc
     9961 tttatttatt aattgtatct gcaattcctt tgtgtcagta gaaattaata atcttttttt
    10021 attaattcta gcctctaata ttgcaggcaa taaatatcct acacttttac ctataccagt
    10081 tggtgcctct atacatgcaa ttttttcttc atcttcgcta tttctaaaca actctcttat
    10141 tgttttagta agctcatatt gaccaggtct atattcatat gtaaatcctt ctttattctc
    10201 ccaaatagtt ttgtatttta ataattcttc ataatttttt tcttcttcat ttaaaatttt
    10261 taaggtttct ctttccttct tcttttcttc ttttgagtca aatatattat attctttttt
    10321 tatatttata ttatttgata aatcataatt tgcctcttct aaaaatttgc tccattccca
    10381 tgttggaaga ttaaattttt taagatatga atttatttta aaactcaatg gctccaaagt
    10441 agttttttca tagttattaa attttactaa aaggcaattt acaatattta ttatatcaat
    10501 agcatctgat a
//
LOCUS       DAGSWI010000137        10378 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.138, whole genome shotgun sequence.
ACCESSION   DAGSWI010000137 DAGSWI010000000
VERSION     DAGSWI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.138"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..828
                     /locus_tag="MJF80_003188"
     CDS             <1..828
                     /locus_tag="MJF80_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBZ0281826.1"
                     /translation="SLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLY
                     DKLRISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAI
                     EKLLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIK
                     LTNINVKLTISKNTWNLSRNQSIALYRLIQESLSNSSRHGKATEIRIFITFNTSNLII
                     TISDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            821..1468
                     /locus_tag="MJF80_003189"
     CDS             821..1468
                     /locus_tag="MJF80_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281827.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1597..2532
                     /locus_tag="MJF80_003190"
     CDS             1597..2532
                     /locus_tag="MJF80_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281828.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINDLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSIIDKNDSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2546..3643
                     /locus_tag="MJF80_003191"
     CDS             2546..3643
                     /locus_tag="MJF80_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281829.1"
                     /translation="MKLYMSVKTMLKGLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3646..4710
                     /locus_tag="MJF80_003192"
     CDS             3646..4710
                     /locus_tag="MJF80_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281830.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            4954..5451
                     /locus_tag="MJF80_003193"
     CDS             4954..5451
                     /locus_tag="MJF80_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBZ0281831.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5627..5875)
                     /locus_tag="MJF80_003194"
     CDS             complement(5627..5875)
                     /locus_tag="MJF80_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281832.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(5941..6957)
                     /locus_tag="MJF80_003195"
     CDS             complement(5941..6957)
                     /locus_tag="MJF80_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBZ0281833.1"
                     /translation="MEYNGLLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7015..7782)
                     /locus_tag="MJF80_003196"
     CDS             complement(7015..7782)
                     /locus_tag="MJF80_003196"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBZ0281834.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7850..10039)
                     /locus_tag="MJF80_003197"
     CDS             complement(7850..10039)
                     /locus_tag="MJF80_003197"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBZ0281835.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3613 a         1590 c         1194 g         3981 t
ORIGIN      
        1 tctcttatta ttagtttaga taaaaatata tatttggcac tggagtcaag ctctatattg
       61 ctaattataa ctgtactttc tatatatata gaaaatgaga atttatcaaa attatacagt
      121 caaaatcttt atgataagct tagaatttct aaagatgaat taaaaaaagt taatgctgat
      181 ttggaaactt atgcaagttc tatagaagaa ttagcaattt taaaagaaag aaatcgaata
      241 tcaagagaaa tacatgatag tgtaggacat tctctttcta caacaataat acaattaaat
      301 gctatagaaa aactcttaaa agacaaacct ctaatttatg acttagtaca tgaacttaga
      361 gaatttgtaa ctgaaagttt tcaagatgta agaagggcta tatctgagtt aaaacctgtt
      421 gaatatgaaa attaccaaag tttatttaaa atcaaagaat tagttaaaag ctttattaag
      481 cttacaaata ttaatgttaa attaactatt tcaaaaaata cttggaactt atccagaaat
      541 caatctatag ctttatatag actcatacag gaatcccttt ccaactcatc taggcatgga
      601 aaggcaactg aaatcagaat ttttattaca ttcaatactt caaatcttat aattactatt
      661 agtgataatg gtataggatg tggcaatata aaaaaaggaa atggacttaa tagtatcaat
      721 gagagaatat atgaattaaa tggtaaagtt gaatttgata attctaataa tggtttcaca
      781 attagagctt cttttcctaa attttcaggg ggtgattttt ttgaataaaa ttaaagttct
      841 tatagtagat gacgaaaaat taattagaaa aggtcttaaa attatattat catcttataa
      901 tgatttagaa attgtaggag atgcttctaa tggatatgaa gctttagaat tttgtaaaat
      961 gaatgatgta gacatagttc ttatggatat acgaatgaaa gtatgtgatg gggttctagg
     1021 tacaaggctt ataaaggaat ataataattc tataacatta ttaattttaa caacttttaa
     1081 tgatgatgaa tatataaaag atgctatgaa gtttggtgct tcaggatacc ttttaaaaga
     1141 tagctctgat gaagtattgc atgaagggat tcgttcttca ttttttggca atattgtgct
     1201 tgataaaagt gtagctgaaa aaatcatgac ctctgaaaaa acggttaaac aagagcatct
     1261 ttatgacatg tacaatttaa cagaaaaaga aatatctata attagactaa ttgcaaatgg
     1321 acttaataat aaagaaataa gtcaagaact attcttatct gaaggtacaa taaagaataa
     1381 cataactaat attttagcta aactagaact tcgtgacaga actcaactag caatctttgc
     1441 tttcaaaaat aaaattgtaa tagaataaat ctaaaaatat aaagtgactt aagtcacttt
     1501 ttttagtgac ttatagtaat agaattataa ttcacaattc tttattataa tattatagct
     1561 ttttaactta tacaaaatta ttttggaggg attactatgg atatagtcag agtaaataat
     1621 ataacaaaaa gatttaatga taaattagta cttgataaca tcagcttttc tgttaaaaaa
     1681 ggtgagatat ttggacttat tggcccaaat ggtgcaggta aatctacact tataaatata
     1741 attacaaatc ttatgttacc aaatagtggt agtattcaaa tcaatgattt agatttatca
     1801 aaagattata taaaagcaaa gtctataata gggttagttc cacaagaatt agcaattata
     1861 gaaactctta ctccttttga taacttagag tactttggag ctttctacgg tttaaaggga
     1921 aaactactta aagaaagaat cattgaagca ttagaagtta caggtcttac tgaagtcaaa
     1981 aagaaaaagg tcaaaaaatt atctggtggt atgcaaagga gattaaatat tggaatagca
     2041 ctcttaaatc atcctaaaat attaatattg gatgaaccaa ctgtaggtgt agacccccaa
     2101 tcaagaaatc atattttcaa ttttataaaa gatatatcta aaaaacatga aactactgta
     2161 atatatactt cccattatat ggaagaagtt gaacaccttt gttctaaaat ttttattatg
     2221 gatgaaggta aagaaattgc ttttggagat aatgattatt taaaatcact cgtatctaca
     2281 aatacaaaat tgattatgga aataaaaaat ataaatgccc aacttatctt tgatttaaaa
     2341 aatactaaag gtgttatatc tgttttagaa aataattcat tattagaatt ggatatagat
     2401 aaaaaattac aacttacaga tatactctct ataattgaca aaaacgattc aaaaataatg
     2461 aaaatttctt atgaagaacc tagtctagaa gatgtatttt taaatctaac aggcaaaaat
     2521 ttaagggact aatggaggtg attttatgaa attatacatg agtgtaaaaa ctatgctaaa
     2581 gggtttaaaa agctccttta tacttaattt aatatatttt ttagctttac ctctgatttt
     2641 atcttggttt cttggtatgg ttacagaaag catgtttcaa aaccctataa aaacagaatc
     2701 tacccccatt gttatatatg acaaagataa cacaagatta tcaaatgatt tgacaaagta
     2761 tttgaaaaat gatttatctt atatattaac agttaaaaaa gatgattcta aggcagaatt
     2821 aaagcttact atacctaaag gctatgagag tagtttatta aatgaaaaat caaatactct
     2881 aaacatagaa aaacttggaa ctcgtgatga tattgcaata ttgcttcaag atatattaga
     2941 tacttaccat gaaaaatttt atttaaataa ttctcagaaa ataagttcag aagatttttc
     3001 taaattattt aataaaaatt ctattgatac ttctataata aataacaatg ttaaacagag
     3061 ctcttatgaa tactttgctt tagtttcact aggatttctt gtaataattt ttataatgaa
     3121 caatatttta tcaaattata taagtgaatc aaagggactt tcaaaaagac tttattctat
     3181 gcctataact agagtacaat tcttaattta tgactttgta ggtttatgga tttattcatt
     3241 tatattctta ttattatatg ttttattctt tagaatactt ggaataacat ttaaaggtaa
     3301 ttttgccata ttgctcctac tttgtgcttt atcatcttat tttatgactt caatatctac
     3361 ctttgtaacc agctttttta gcaaaaaata cggtacagca atagtttatg cattactctt
     3421 tttacagaca atttttggag gtatcttcag tatgataagt gatacattta ctaagcttac
     3481 aagtttatct cctacctatt taataggaga attgtttagt aattatgaaa catttaaaac
     3541 tatagattca ataggagatt taattttcac atgtttaatt atctctacaa tattaattgc
     3601 acttgctatt gtaaaggaaa aatataaatg gagggaggtt taataatgat tagattaact
     3661 attgcaaata taaaaagata tctaaaaaat catactcttt taattaacat ggttatgctt
     3721 cctattatac taattttttc acttaatttt tttatcaaca actcaggaaa tcaatcaatg
     3781 tacttttctc cagtagcaat tgtatctgac tcttcaggaa agtatgaaca taagttaata
     3841 aatagttcta aattaaaaga aaattcattt agattaaatg aacaagataa ggctatgaat
     3901 ctattaaaaa ataataaggt ttctgctgta ttcgttttag ataaaaattt ttcaaatagt
     3961 atagataaat tacaacgacc tgtagttaaa tgctttaaaa ttgaaaatgg tggtggtagt
     4021 ctttgggcag aatcacaaat agaatctttt attactaaat cgttaaaatt gaaagtggat
     4081 aaaaatatag atgataaatt gacaaagaca aatataattg ataacaaacc agataagaat
     4141 aaaggttcat ttttagtagt cttcttgata tgctatttta tgtatattaa tgcggcacat
     4201 cttgcttctg atttatttac attaaaaaaa tcaaatgtgc taaaaagact tatctctaca
     4261 gataataaag atataaaaat aattttcagt atatttttag gactattttt tattcaatca
     4321 attgtataca cttttgctct attatgtttt tcaattatag aagactttaa tttgagtctg
     4381 aatgtattga tgattgtact agcaaatagt tttgtttcca ctggatttgt attttgggta
     4441 gctagagtat ttaaaaatga aagttctata agtcttatat ctacatttta ttctttaata
     4501 ggtctagcta tatctatctc tagtctaata ccatcaatgg ataaattatc ctttatgact
     4561 aatctttcaa agttcacacc attttattgg actatagatg ctattaaaaa caatggtagt
     4621 attttaataa atataatagc tctaatatta attggaatta tatttgtaac tgcaggaagt
     4681 cttaaactaa gagattttgc taaaaactaa taattattaa atataattag tatctatttt
     4741 tatataattg taaaagaata tttcaacata ttaaattatt ttatcttata tttttctctt
     4801 tagtttcaca catcaccaag tctaaacttt atattatata ttaatatttt tgacttgtca
     4861 agttatttat gttataatat taatcattag ataattaaag cattgtacct aagttgcaat
     4921 atctaaaatt caatttggag gtataactag catatgtata aaattggtga aagtgtaatg
     4981 tatccaaaag agggagcctg ctctgtaaat gatattgtaa caaagaaaat aaatcatgag
     5041 atgcaaaaat actatgaatt aagtgtcatt tttaatagta accttaaaat ttcgattcca
     5101 gttctaaatg cagatagaat tggcatacgt ccagttatgg atggaaatga tgttgataat
     5161 tttatccaat ctattaataa aacagatggt gtatggattt ttgatagaaa agaaagactt
     5221 aaactgtacc aagataaatt tcattcagga gatgtttttg aaattgtaaa attaatcaaa
     5281 atgcttatga tacaagacag ttcaaaacaa ttgtgtagca cagataagga gtttttaaat
     5341 aaagctcaaa aatttgcttt atcagaatta gctgctgctc aatgtaaatc ctatacaatg
     5401 gtattagagg aaatgaaaaa acatatatta aattcaaaga atacaaatta aaccagtttt
     5461 ttaaactggt tttatttttt atactaatca tccatgctac tttagcttat tttaaactgc
     5521 ctaatctaat attaataaag ttatctcaat tagatagatg tattttattc aaataaaaaa
     5581 aactaagagt aaatcatatt actgatttac tcttagctaa taaaatttaa tcttcttcaa
     5641 gttctttttt ctcttgtaat aatacactta attcatttgc ttctaaaact tgtgcctctg
     5701 caatatcaag taactcttgt tgccattcca cacatttatc tttgtaatca aaaggtagcg
     5761 ttctgtcaac tatgtactta tcaatacagt cctccgcact tttaattatt tcttttcttt
     5821 catcaataaa tttttctaat tctactaagg cttcttcata agcttttcta ctcatataaa
     5881 ttcccccttt gtacaattat tctacattaa aatgattttt cctctttttt gtataaaatt
     5941 ttatttgtat ataacttttc catcaatatc ttttatagtt tgacatccag ttaatatcat
     6001 agaactactt agctctgatt gaagtttatt tacataagtt tctacaccat cagttgcacc
     6061 accaaaagaa gcagtcacaa aaggtcttcc tattaatact gcatctgcac ctaaacctat
     6121 catttttagt acgtctaaac cagttctcac tccaccatct gcaagtattg taactttacc
     6181 tttaacagca tcagcaattt ttggtaaaac ttcacatgct cctggtgtac agtcaagcac
     6241 tcttcctcca tgatttgata caactattgc atccacacct gcttctactg ccattaaagc
     6301 atcttcaaca gtcattatac ctttcaatat aaatggtagt ttagtagact ttactaactc
     6361 ctttatttgt ttaacatttt taggaagtac tggttttcca tgtaatgata aagttactag
     6421 accacaggca tctatatcaa ctccaactgc aaatgcacca gcttcttcag ctaatcttat
     6481 cttttttata atattttcat tatcccaagg tttaataaat acaattccag ctccattata
     6541 ctttttgact acatctaaat tttccattaa aaaagcatct actgccgtat caccaaccat
     6601 tgcataaata ccactattag cacatcctgc aactactggt tctatgtaat ctctttcatt
     6661 aatttttcca cccatattta aagtagttcc tgttactgga gctgcaaata taggtgcact
     6721 cattttcttt ccaaacatct ctatagatgt atcaggattt gatacatcat gtattactct
     6781 catatttact tttacttttt ctaaactctt gctattttct ataaaagaag aaccacttcc
     6841 tttacctccc atgccaggca cttcacctgc acaagcaacc ccattacaaa ccttacaaac
     6901 tttacaactt ccatttaaat tttctctagc actttttaat aatccattat attccatctt
     6961 taatccccca ttaactatag taatttatat tatatacatt atatcatatt atatttacag
     7021 tatatttttc aaaaaacttt ttctgtggat aggagttatg ccatattttt caatagcttc
     7081 atagtgttct ttagttccat atcctttgtg agatttaaac ccatattctg ggtatactct
     7141 atcatattgg tacattatac tatccctagt aactttagca agtatacttg ctgctgctat
     7201 tgatatagat tttgaatctc ctttcacaat tggattttga gatatgtcaa tacctggtat
     7261 tgttgctgca tcaactaata aataatctgg tgcttttttt aaacaattta tagccttttt
     7321 cattgccata tatgtagcat ttaatatatt aaattcatct atttcttcat tgtttactat
     7381 acctattcca taatctagtg cttcttcttt tattacttca aaaagttcat ctctttttgc
     7441 ctcactcagt ttcttagagt catttacacc ttctattttg gtatcttttt taaatactac
     7501 aacagaagct acaacaggtc cagcaagtgg ccctcttccc gcctcatcaa ttcctccaat
     7561 atataaataa cctttatcat agccttcgtt ctcaaatata tttatagttt ccaatctttc
     7621 ttcttctttt ctaatattat ccagcttttt tgctagtttt atagctagac cttgtacaga
     7681 ttttctttca tcaactctta acagctctat atattccata tatttttcta cttctaaagt
     7741 ctctattatt tctttaatct ccctcacact tttatcctgc ataccattcc ctcatctttc
     7801 gtttttaaaa atttaatttt taaatatatt aaaatttttt aattagagtt taattgcatc
     7861 cactatagcc acattttaaa catatactac atccacctgt atgtgcaatt ccttcacttc
     7921 cacactcagg gcacacaaat ttttcatctt cttcaaaatt tttatttatt atttctttgg
     7981 ctttttcatc tttattatct ttttcttttt tattattttt ataatattct tttttactat
     8041 tttctttttt atcttcttta ttattttctt cctttttttc atttattaaa attccactcc
     8101 ttttacatcc atctctaaat attgttactc ctttaagacc agcctcccat gccttaatat
     8161 atatattgaa aacatcttca actgtagttt caaaaggtat atttattgta gatgatatag
     8221 aagcatcaat atacttttgc catacttttt gcatgtttat tctttcttca tatcctatat
     8281 tcatagcagt aacaaaaaaa tctggtaaac attcttcatc tgtaatacct ttcttgtcca
     8341 tatactcttt aacaattgga gtatatactt tataatacac atcctcatca tgcaagcttt
     8401 cagtttttct tatatatgaa agattaaata taggttctat tccaccactg atacctatca
     8461 ttgtagatat ggaacctgta ggaggtatgg ttaaaagttg tgaatttcta agcccatact
     8521 ttttaaccat ttccttagtg tcttcattta gattttctat aaaaaactta gatttagaaa
     8581 tattttcata tttatactca ctaaatgttc catattcttt agcaattaat gctgattgct
     8641 tcacagcttc atttaacata gtcttaccaa ttaaatcaca tagttctaaa gattcatcag
     8701 aaccatatct tattcccatt tttataagca tatcagctat acccattaca cctaaaccta
     8761 tctgtctata cttatgtaca ctatctcttt gttcttgtaa tggatgcaag ttcaatccct
     8821 catctaagac atcatttaaa gctactacag cttctctaac gcaagatttt agtttattaa
     8881 tatcaaaagt agcatgttca ccaaatggtt gtattataaa ctctgacaag tttattgagc
     8941 caagtagaca acttcctcca cttggaagtg gttcttctgc acaaggattt actcctgaaa
     9001 agcaaaagtt cttatcttca ctcataaaat tatatgtttt tattttatcc caaaacaata
     9061 tacctggttc tgcataatcc cagttatttt tcacaaattt attaaataaa tcataagcat
     9121 ttactatctt aactattttt tctccagtag tttcaacttc aaaagtacaa ttatataact
     9181 ttttttctct tacagcttgc ataaattcat ctgttactct cacagaaata tttgctttag
     9241 taaccttctc caaatctgtt tttatatcta taaagtcttc tatctctgga tgatttacat
     9301 ccatagaaat cataagagct cctcttctcc ctctttgacc aattaagcca gttgtcatac
     9361 tgtaaagttc cataaaagag actgctccag tcgtatgttt tgcagcattg ttaactttcg
     9421 ctccttttgg tcttagcttt gaaatatcaa ttccaactcc tccaccataa ctaaaagttc
     9481 tagcaagttt ttttgcagta tcaaatatat cttctatgct atcttttggt ggttctatta
     9541 catagcaatt agaatatgtt actttttttc cctctttctg taatcctcta tttgctaaaa
     9601 ttcttccagc aaatagaaat cttttttgtc ttattagttt tgatattctt gaactattat
     9661 ttgaaattct ctttatccat tcatcaaaac tctcaccatt atgtctatac ttgttcttcc
     9721 atattgattt ttgtaattca tcaaactccc aagctttagt ccttatttca gagcgtcttt
     9781 ctctatataa tatgtagtgt tttgctatat cttttctatt tgattccata agtagtactt
     9841 caactatatc ttgtatttct tctacggata agcatttttc catagaatta tagtcattag
     9901 ctatttttct tgatatatca tatgccaact ctctatctac gccaccttca ctatttatac
     9961 tagctttata gacagcatct tcaatcttac ttttactaaa aagttctttt gtaccatctc
    10021 tttttattat gtacttcatt tttaaacctc caataaatta gtattaaatt aatacacaca
    10081 ttaactataa ctctaaacaa actttttata tatttgtata ttttcatatt aattatctaa
    10141 cataatatat attatactat tttatattca atataacact ccttatagtt ttcttaactt
    10201 gtaaaatatc ttcctaaaaa actcaaaaat aatataaaaa agtatataaa agtcaaatta
    10261 tactcttata tactttattt ttttatattt ttttcatttt aactttgatt aattaataag
    10321 tatttagaaa ataaattctt tattttttct ttacaaaaat atgtattagt gtttttat
//
LOCUS       DAGSWI010000138        10329 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.139, whole genome shotgun sequence.
ACCESSION   DAGSWI010000138 DAGSWI010000000
VERSION     DAGSWI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10329
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.139"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            98..2599
                     /locus_tag="MJF80_003198"
     CDS             98..2599
                     /locus_tag="MJF80_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBZ0281836.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2759..3427
                     /locus_tag="MJF80_003199"
     CDS             2759..3427
                     /locus_tag="MJF80_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0281837.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3411..4688
                     /locus_tag="MJF80_003200"
     CDS             3411..4688
                     /locus_tag="MJF80_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBZ0281838.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5089..5310
                     /locus_tag="MJF80_003201"
     CDS             5089..5310
                     /locus_tag="MJF80_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281839.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(5599..6363)
                     /locus_tag="MJF80_003202"
     CDS             complement(5599..6363)
                     /locus_tag="MJF80_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBZ0281840.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            6637..7311
                     /gene="fsa"
                     /locus_tag="MJF80_003203"
     CDS             6637..7311
                     /gene="fsa"
                     /locus_tag="MJF80_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBZ0281841.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(7433..8827)
                     /locus_tag="MJF80_003204"
     CDS             complement(7433..8827)
                     /locus_tag="MJF80_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBZ0281842.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(8905..10251)
                     /locus_tag="MJF80_003205"
     CDS             complement(8905..10251)
                     /locus_tag="MJF80_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBZ0281843.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         4201 a         1230 c         1602 g         3296 t
ORIGIN      
        1 atatagattt ctaaataagt aatttatcaa tattacatat caaaataaaa cgttacaaat
       61 tagctgaaaa tatattgaaa gaaaggaaga ttttattatg agttcaaata aagtggtaga
      121 aagttataaa attacaggta tgacatgtgc agcttgtgca aaggcagtag agagagtaac
      181 taagaagatg gatggagtat atgaccaaag tgtaaatata gctactgaaa aacttaaaat
      241 agaatatgat aattctaagg ttaattttga tgatataaag caggttgtag aaaaagcagg
      301 atatggaatt ataaaggaag aaagtaataa aaaaattgat atgaaaattg atggaatgac
      361 atgtgcagct tgtgcaaaag ctgtagagag agtggtaaaa aaattggatg gagtagaaag
      421 tattagtgtg aatattgcaa cagataaagc taatatagac tatgacccat caaaagtaaa
      481 attatctcaa ataaaagcag ctattgaaaa agctggatat aaaccaatag aagaggtaag
      541 aaataaggtt gatgtggatg aagataaatt gagaaaagaa agagaaatga agtctttatt
      601 tgttaaattt atagttgcaa tagtatttgc agtacctctg ttttatatag caatgggacc
      661 tatgataata aagcctatag ggccatggcc tttgccagaa attataaatc ctatgacaaa
      721 tacttttaat tatgcattaa ttcagttaat tttagtaata ccagtaatga tagctggtta
      781 taaattttat ataaatgggt ttaaatcact tttttcttta agccctaata tggattcttt
      841 agttgcaata ggaacattag ccgcattttt atatagtcta tatacgacac tacaaatagc
      901 caatggacaa attcaaggta tgcaccatca tcagttgtat tatgaaagtg caggaataat
      961 aatagcactt atcttacttg gtaaatattt agaatcaaaa tcaaaaggaa aaacatcaga
     1021 agcaattaaa aagcttatgg ggctacaacc taaaactgca atagtgttag ttgatggtaa
     1081 agaagttgaa actcctatag aagaagtaga gataggagat atactattgg taaaaccagg
     1141 aactaaaata cccgtggatg gagttgtaat agaaggatat acatcagttg atgaatcaat
     1201 gcttacaggt gaaagtatac cagtagaaaa gaatgttgga agtaaggtga caggagctag
     1261 tattaataaa aatggtgtta ttaagtttaa ggctgaaaaa attggtggag atactgctct
     1321 tgcccaaata ataaagttag ttgaagatgc acaaggtaca aaagcaccta ttgctaaact
     1381 tgccgatact gtatctggat attttgtacc aatagtaatt gcaatagctg tagttgcatc
     1441 attattgtgg tttttaatag gaggaaaaga tatagtattt gtacttacta tatttatatc
     1501 agtacttgta attgcttgtc cttgtgcatt aggattagca acaccaactg ctataatggt
     1561 tgggactgga aagggcgcag aaaatggaat cttgataaaa ggtggagagg cactagaatc
     1621 tgcacataaa gtaaatacag tgatatttga taagacaggt actataacag aaggaaagcc
     1681 aaaagtaaca gatatagtat taaataataa tgtaaaagaa gaatatctta tcaagattgc
     1741 ttcaagtgca gaaaaaggtt cagaacatcc tcttggagaa gcaatagtta agtatggaga
     1801 agagaagaat ataaaatttg aaaaagttga taattttaaa gctataccag gtgctggtat
     1861 acaagttact attaatgatg aaagtatatt acttggaaat agaaagctta tgaatgacaa
     1921 taatataaag cttggggatt tagaagaaaa atcaaatata ttggcaagcc aaggtaaaac
     1981 acctatgtat atagctgtag atggaaattt atcaggtata atagcagttg cagatgtagt
     2041 caaagaaagt agtaaaaaag caatagaaat acttcatgat atgggaataa aagttgcaat
     2101 ggtaactgga gataatgcga aaactgcaaa tgctatagca aatcaagtag gtatagatat
     2161 ggttttggca gaggttttac cagaagataa gtcaaaagaa gttgaaaaac tacaaaatca
     2221 aggaaagttt gttgcaatgg taggggatgg aataaatgat gccccagctc ttgcaaaagc
     2281 agatataggg atagcaatag gaagtggaac ggatgtagcc attgaatctg cggatatagt
     2341 acttatgaag agtgatttaa ttgatgttcc aacagcaatt aaacttagtc atgaaactat
     2401 aaaaaatata aaacaaaacc tattttgggc atttggatat aatacaattg gtatacctgt
     2461 agcagcagga atattatatg tatttggagg acctttatta aacccaatga ttgcagcagc
     2521 agcaatgagc ttaagttcag tttcagtcgt atcaaatgca cttagactta aaaacttcaa
     2581 ggcatataag agagattaga ttaataaatt aaataaaatg tatccaaaag agctatcttt
     2641 aaattaaaag gattaattta caaattaaga tagctctttt ttcatgtaaa attcacaaca
     2701 ataagataaa atataagtaa tatacgaaat tttaaataaa atagaaagga ttgatagaat
     2761 gaattcgtca atacttgtaa ttgaagatga ttcaaatata caagagttaa tatcagaatt
     2821 tttaagtgca gaaggttatc aagttgatac agcaaatgat gggttggaag gtatacaaaa
     2881 gtttaagcaa ggaagttatg atttagtaat attagatata atgatgccaa acttagatgg
     2941 ttatggagtt tgtaaaatga taagaaagtc atcaagcgtt ccaattatat ttttaactgc
     3001 tttaaatgat gaaggagacc aacttaaagg gtttgactta gaatgtgatg attatataac
     3061 aaaaccattt tcatttaatc ttcttataaa aagagtagaa gcaattttaa ggcgaagtaa
     3121 caagactata aatgacaaat ttatagtttt tgaaaaatta aaattagatt taaacacata
     3181 tattgcagag atagatggag aacctataga acttacacta aaagagttta atatattaaa
     3241 agctttgata gaaaagtatc cacaagttat aactagggaa ggtcttttgg atagtatatg
     3301 gggttatgac tattatggag atactaggat tgttgatgca catataaaaa atataagaaa
     3361 aaaaatatca ttaccatata ttaaaactgt aaaaggaata ggatatacac ttgagaagga
     3421 tatttgataa gtgggaaaag ttaagtataa agtataagtt attttcaata acgacatcat
     3481 tattaatagc attagcttta ataatatatc taattttata ttttttattg ccatcatatt
     3541 atcatgagta taaaattgag tctcttcaag aaagtttgaa atctttagta gatagttcaa
     3601 ttcatttcga tacatatact ttagaagaaa gactttatta tatggctaaa gaccaaaatt
     3661 tggctatttt attaaaagat aaccaaggta aaattgtata tggtaagaat gaagtagtta
     3721 ttttaagata tagtaagtac atgattaata gtcttgagga tgagtataga acttctatac
     3781 ctatatatac taaagatgca aaagatggtc catatacttt agaactcgtt atgccattac
     3841 aaccaattga cgaagcaaac gaagttataa gaaaactaat gccatacatc atatcaatag
     3901 caatattgat agcgattatt ggagcatata tatattcaat tgtaataaca aagcctctta
     3961 taaacataat agagagtgag agagaacagg aatatagaag gaaggatttt gttgctacaa
     4021 tatcacatga actaaaaact ccaataacca taataagtgg tcaaattgaa ggtatgattt
     4081 atagtgtagg taagtataag gatagggata cttatctgaa aaaatcgtat gagtgtacac
     4141 aagaattaaa agatttagta aatgaaatga tagaggtttc aaaatcagaa atacttgaaa
     4201 aagatttgaa attggtatca ataaatataa gtgaattatt aaatagactt gtaaagagac
     4261 aagtattttt aatagaagaa aaacatatga aaactatttt aaaaatagaa gaaaatttgg
     4321 aaattaaagc agaccaagag agaataacta aggcaatcaa taatataata aataatgcca
     4381 taaaatattc tcctgaagaa tcagagctta taattagact ctatgataaa aataaacgaa
     4441 taagtaaaaa aacttctaat caaagagtgg ttttagaaat tgaaaatact ggtgtaacta
     4501 tcgagaaacg ttatttagaa gaaatattta atccatttta tagaatagaa aaatctcgtt
     4561 caagaaaaac tggaggaagt ggacttggac tttatatagt cagccaaatt tttaaaagtc
     4621 atgggtttga ttatagcata aaaaacaaag aaaactctgt agtattcaca gtagaattta
     4681 aaaattaatt acttattata aagaatatat aactttcaat aatagttcat tttaaaatca
     4741 caaaaaattc ataatgaaac tgtattatat aaatataaag aagcttaaaa ccataaaata
     4801 caccaactat aatgatgaga tttccttaca tcctccatat aagtcatcat tatagtaacc
     4861 caacaattaa gttactatga atttaaaaaa taacaccgag catataatct atgcttagtg
     4921 ttatttttat tttgaaataa aaaatattta atttattgaa atgtatagat ttagatagtt
     4981 taaactatat ttaagactta aaaattataa ttgatattac aaataaatat atgtactttg
     5041 ggaaaaataa atataggaag tattatattt acagaggagg tagttttgat ggatagatta
     5101 aaaagagata tgaaaaatat gaaagaagat ttaaaagaag gtgttaaaaa gacagcttat
     5161 gttatgaaaa atacagctga agatttgaaa gatgatgcac aaggtgctat ggtaaactta
     5221 gaaatgaaaa aagatgagat gatggaaaga tatgaacaaa agaaattttc taaagaatta
     5281 gagaagcaaa tgaagaatga aatgaaataa gcttttattt atgaataaca aattattgta
     5341 ttcatataaa aagtagccag taatattaaa tagtttattt aaggaaatag actttctaaa
     5401 aataactgta ttagatttag aaagtctatt tcaaattagt tttataaata gacataagga
     5461 taaagtagat gttatagaac tagattttaa aaaatagata ctaaggataa aataaatatt
     5521 atagaaatat gtttttaaga atatttatca aataaaaatt tatatttata agaaatccta
     5581 taaaacatat aattaaactt ataaaacatt tacattaaat tctgataaaa tatctactat
     5641 atcaggagta ggcgtttttt ctgtaattat agaactaata tcttctaaat gagcaaactt
     5701 gaaatttcca tcaaatttaa actttttatc ttccataact ataaatactt ttttacttga
     5761 atttattata gccttcttcg taagaccatc ttcaatttca aaagttgtaa gtgaagagtt
     5821 gaatacatct actccacaac ttcccataaa agtcttatca aacttatatt tcgatataaa
     5881 ctctatagtt ggagtaccga caaaaccgtc taaactgaga tttaataaac ctccagtgga
     5941 aattatattt aaattatttg gttttgaagt aactacattt agtatatcca gcatatttgt
     6001 aacaagtgtt acttttttat tactttcagc taacaacttc gcaagcaaga tgttaattgt
     6061 agagatatct aaaaatatag tttctctatc ttcaattaaa ttaaatgctt tttctgctat
     6121 tattttcttt gttggaatat taatttcttt tctgtctttt gtatcctgat tctctagtgt
     6181 ttctctagct agtacagctc ctccataaat tcttcttatt gaagaaatct tttctatatt
     6241 ttttaaatct tttcttatac aatcttctgt aacattaaat ttagaactga gctctttgac
     6301 agtaactcta ccttcttttt ttaggatatt taatatagct tggattcttt cttctgcaaa
     6361 cattttctac ctcttttcgc aatatagtat tatagtaaat attataagat aaaatacaat
     6421 atatcaacta ttttaacatg aaatacaaaa ttaatttaat ataaaaagta tgttttttct
     6481 aactatatat ttagaattta gtaaataaaa aaattataaa actaagaagg aaaagtaaaa
     6541 caataataaa aaataaaaaa caataagcaa aatgttgaaa ataataccaa atcaatatat
     6601 aataagaata aagaaagatt aaagggggat attttaatgc aactaatctt agatacaggt
     6661 aatgtggagg agattaagga gttatgtact tgtttaccaa tagatggtgt aactacaaat
     6721 ccaagtattg tttcaaaaga aaaaaagaat tttaagcaac ttataaatga aattggtgag
     6781 attataggtg aggatatgcc tatacatgct caagtgttaa gtacaaagta tgaagaaata
     6841 ttagaagaag cattatatat aagtagtttg agaaaaaata tatatgtaaa aatacctgta
     6901 acacaagatg gtttaagagc tataaaagac cttcacagaa aaggtgtaaa aataactgct
     6961 actgcaatat tcactgctca tcaaggtttt ttagctgcaa aggctggagc aaattatatt
     7021 gcaccttatg taaataggct tgataatata tctggtgatg gagtagctat ggtttcagaa
     7081 cttataaaaa taatagatac ttataaaatg gatacaaaag tacttgcagc cagttttaaa
     7141 aatgcacaac aagttataga attaatgcaa catggggtgc atagtgttac agtaccatat
     7201 gatatttgta aatctatgat gaatcatcca ttaactgatt ggagtgtaga taagtttata
     7261 gaggattggg agaatacttt tggaaaagga agtaaaacaa ataatattta gtattatggt
     7321 aaaagagcag ttttgtccaa ttttacaaga acttatataa aaagatgaaa atatgaattt
     7381 tctttttaat aatttcgtta ttatcttaag tcaattcata ttttcatctt ttttagattt
     7441 ctagtcctat aaatttccac caagggaatg ttatacatat tattataaag taggttaaag
     7501 gaatcaaagt taaaccgtat ttgaaaataa ccttgctatt atacagattt tcaccatgac
     7561 caaccattat tgtagcatga tgaaatggta atatgtactg tatgcttact ataatgtaga
     7621 caaatagaca taaagtaaca ctattttcct ttagtatatg taaacttcca agtaatggta
     7681 ttactacaga tgatgtagtg acagcacttc caagacatat attcaagatt attgtgagag
     7741 ttatcaaaaa caatattgaa ataatttctg aagagtttgg tataaacttt atcaaataat
     7801 ttcccattat atctacaact ccagagtact ttaacactcc tccaattgaa aatgctgcta
     7861 taaaaaaaat aaggaccttc caatttattt cttttaaagt actaaatcca actatttttc
     7921 ttagtaataa aagaagtaca caaattgaca ttataattaa tgtgtttatg ttatgtatga
     7981 actgagttaa aaacataaaa aacattattc ctaggcataa aaatacgtgt ttttgctctt
     8041 tgtttagcaa attaaattct ttttttaaat tagaatatga aaaatgaagt ttattaccct
     8101 caagattttc attagaaata aattttattg aatgcaattc ttttttaaag acaaagataa
     8161 ataaaaaata agttataaaa caagtaatta tagttggaac agacatataa aaagcccatt
     8221 gaccccaatt tatattacaa tttccaagtt ctagtacaac ataatttaga agagtgtctc
     8281 catttacaaa aaacattgat gtaaaggtag atgctgtaaa tatactaaat aaaagtatag
     8341 attttgaatc ttcagttatc tgttgttctt ttaaaaactc cttgtaaaaa gcagatacaa
     8401 gaataaccct tggaaatggt tgtgggataa aaaatattaa aagtatacct aaaatatacg
     8461 ataaaagaat cattttttta ggggtattta cagtatttaa aattaacttc tcagaaataa
     8521 tttttgcgac acctgttttt gtaactgcat tagtaattat gtatgcaaga attattatgt
     8581 aaaaattttc tgtaaataaa aactgtaaaa taacctttat tggtgcaatg ttaaatataa
     8641 acgctagaat tacaaaaaac aatgcaacaa aagttttttc aactgagtct acagcccata
     8701 tcaagatagt taaaaaagat aagcatatag aaaattttat gttgaaactg taattaagag
     8761 agaagtagag aattatggta gctataacag tcatataaaa ttgttttggc ttaagaataa
     8821 tacccatttt tatattcacc tccataattt attatatcat aatggaggtg aatacaatga
     8881 atgtgaatac attttataaa aagattattc attgattcta tgatttacaa tagctttagt
     8941 tttccaatgt cctttcttat agtagataac tgtaacaata agacctaaag tccaaccaaa
     9001 aggaataccc atccaaattc cgtctgttcc aaaaaattta gaaagcatat aagaagctgg
     9061 aagtcttcca agccataaag atactattga gcttataagt gcaaacatgg aatcaccagc
     9121 acctttcatt gcacttgaaa gtacaaaaga agttccaata aataaaaaga atggtcctat
     9181 tataaataag taagaagagc caattttaat gacatcttta tcagtattga atagagcaat
     9241 tagattttcc ctaaatagca aaattaaagc aataactgta actgctaaaa ctaaggtcat
     9301 ttttatagat tctcgaatgc cttttttaac tctttcattt tctccagcac ctatgttttg
     9361 agcaacaaaa gtagaaacag cagaaccaag attcataata ggtaagctta taaaattttc
     9421 tatcctaaga ccagcaccaa aagctgccat agcagatgta ccataactat taactaaaaa
     9481 ttgaagagcc atatttccaa ttgaaaacaa catttgttga actccacttg gaagtcctaa
     9541 tctaagtcca tctttaaata ttttgttatc atatacaatt cctttgattt taaatttaag
     9601 cacttcatgt tttttattta aataaattat acttattata aatgatactg cctgagccat
     9661 tatagtggca agtgcaacgc cctcaacacc cattcttagc actacaataa aagttaagtc
     9721 taaaactata tttagaattg tggctattat cagaaaataa agtggagtta ctgaatctcc
     9781 aagtcctcta agtattgcac ttactgaatt atatccagca gaaaataata ttccactaaa
     9841 tattatagta agatacaatt ttgattgagc aaaaacggat tctggtgtat tcataagctt
     9901 aagcatgggg ccaccaaata ctattccaag aatacttatt aatatagaac caataaataa
     9961 aagtatatat gtagtatcaa tagtagcttt tacctttttt aaatctcctg agccataaaa
    10021 ttgtgacact aaaatagaaa atccaaggct aacacccata agcgctgcca ccaataaaaa
    10081 cattataggg ttagctccag agactgccgc cattgcttct tttccaataa atctaccaac
    10141 tattatactg tcagctgtat tgtatagttg ttgaaataaa ctaccaataa gcataggcat
    10201 ggcaaaaaag aatatggact ttccctcatg acctgtagtt aaatctttca ccttattcct
    10261 ccaaataaaa aaattattaa tttattatag tataatctta acataaactt tatatccaaa
    10321 ctataaata
//
LOCUS       DAGSWI010000139        10263 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.140, whole genome shotgun sequence.
ACCESSION   DAGSWI010000139 DAGSWI010000000
VERSION     DAGSWI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.140"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            198..1079
                     /locus_tag="MJF80_003206"
     CDS             198..1079
                     /locus_tag="MJF80_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBZ0281844.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESIKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            1288..1437
                     /locus_tag="MJF80_003207"
     CDS             1288..1437
                     /locus_tag="MJF80_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBZ0281845.1"
                     /translation="MTTVAEGIETSFQKDFLQEIDCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(1697..4264)
                     /locus_tag="MJF80_003208"
     CDS             complement(1697..4264)
                     /locus_tag="MJF80_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBZ0281846.1"
                     /translation="MIYYVLPLEHKQATIEIIGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKTAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     FLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPMHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSSAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(4267..4872)
                     /locus_tag="MJF80_003209"
     CDS             complement(4267..4872)
                     /locus_tag="MJF80_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0281847.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTNTALVT
                     RLLINSMLSISYTSRPSDSLEKMLDETMNIFLHGIMNDGGE"
     gene            complement(5246..6706)
                     /locus_tag="MJF80_003210"
     CDS             complement(5246..6706)
                     /locus_tag="MJF80_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBZ0281848.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRGNIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            7076..7654
                     /locus_tag="MJF80_003211"
     CDS             7076..7654
                     /locus_tag="MJF80_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0281849.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            7945..9195
                     /locus_tag="MJF80_003212"
     CDS             7945..9195
                     /locus_tag="MJF80_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0281850.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAVPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            9356..9925
                     /locus_tag="MJF80_003213"
     CDS             9356..9925
                     /locus_tag="MJF80_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBZ0281851.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3444 a         1752 c         1484 g         3583 t
ORIGIN      
        1 taatttttat tatttgtact tatttaaagt ttcatctaat tataaattgg acaattttta
       61 tgttgatttt attcatacaa attaaagtaa cttataaaat ctccacaata aaaaacattt
      121 tttattcact aaactgctcc atcattggtt tagcaataaa tatatttttt aggtctttaa
      181 tgtcaatagt gttaaatatg ccagcatatt tatttgatag taattctatt gcaaatattt
      241 acagagtatt tcctatatca tttagcttta tagttacagg actatatttc agttataaaa
      301 ataataaaaa taataagatt aactttgagg cagttttaga ggacaaaaaa aacttaattt
      361 tcttattgag ccttatgttt ggaatgtact tgtatctatg tttgagtttg tcaatatact
      421 atactgttgg aaatgtattg attttaaaat tatatggaat caagtctgcc ataatagttt
      481 tggtaggtaa ctatattgca gaaagatata gctttagaat tactcaaata aaaggttatg
      541 atattaaaaa tcaaatagaa tatgtagaaa gtataaagac agagtatgaa attaataaat
      601 tgaatgatat tgtatataca gatttactga caggcttcta caatagacct tttgtaaatc
      661 aaaaactatc agagtggaca tcaaatcact acaaatttac cttatgtttt actgatttaa
      721 atagcttaaa acctgtaaat gataaatttg gacaccaatt tggtgataaa tacatatcca
      781 ctactgctca aataataaaa aaacttataa atgtagaaaa ttccttacta tttagatatg
      841 gtggagatga atttttattc cttttacaga atacaagttg tgtagaagca gaaaagataa
      901 tgattaatat aaattatgaa ttatcaaaat taagtaatac tgacgagtat ccttatgctc
      961 tttccataag ctatggatta gttgagtggg atggaatatc cgatattgaa actttaatat
     1021 caaaggcaga ttctttgatg tatgaaaata agcttaaaaa taaatctact ttaaactagc
     1081 tagttaagat attctacaaa atatatgtta taagagagta ctaagaatgt caaaatatat
     1141 agattttgca aatattgaat acagtttact aatgaatgca ctggatataa gtgttagtaa
     1201 acacctatta gatgaacact ttactgttgt ttgggctaat gactgttatt atgaaatgtt
     1261 tggctatacc tagctaaaaa gttaaaaatg accactgtag ccgagggaat agagaccagt
     1321 tttcaaaaag atttcttgca agaaatagac tgtgatatgt tacaaggata cgtgttctct
     1381 agacctttac caataaaaga ctttgaaaaa cttatgtttc aaaactcctc caattaacaa
     1441 tataaaattg cttattataa gtgatattat tgtatcaaca aataaaggaa ttgtctcaaa
     1501 attgattttt tctaatcaaa tttttatttt gagacattct catttttttg cataagaaaa
     1561 tccccaccaa gctacggttt ttcttaccgc tgttcggtgg gtattagtag tattatgaaa
     1621 tctgaaaact ttaagtatta ctacttccgc tagcgcaatc acaaaggtcg aaagcttcaa
     1681 agcgaaaata ggctttttac tcatcaaacg tcaccgtacc tgccaacccg tccaccgtta
     1741 tcatttgtcc gctcttaata gttcgtgtgg caatgtgtgt agccataacg gctgggatgc
     1801 catattcacg tgcgacaatg cttgaatggc taagaggacc accaatgtcc gttacaatcg
     1861 cacttgcaga tgaaaataat ggtgtccatg ctggagttgt ggtaactgca accaaaacac
     1921 ttccaggttg aaagctttca aaatctgctg gactattcag tacacaagct ggagctgtca
     1981 caacaccagt actggtacca atgcccgtca acacaatttt cccatctttc tgcttctgtt
     2041 gtgcttgtga tatagttttt ttgttttttt ctggcaactt ggatggtgac acataaccca
     2101 tgtatttttt aagttcagct tttcgaacag gtattatccc gttcatatca gatagagact
     2161 tgtttttatc caactgctct ataagtacct ccaattccga ttttgtgagc caataaatat
     2221 catctaaatg tgaaatagct cctcctcgta tgaatcgttc tgagatttcc tgaaacatac
     2281 aacgaatcag aggatgtccc attcccatca aataaatagc attttcacgc ataggagagg
     2341 tttcctgtgc ccaatggagc aacttaaaaa atagtttttt acgcaatcca cctatgtgtt
     2401 gtaaaatctc ttcttctgca tgttttctac gcttttcaaa cgtggtctgg cgtaaaaaag
     2461 gactttcccc ttttccttcc acaaatgttt ttacggattc aaaagttggt gtaagtgttt
     2521 cctgcggtgt ggaatgcgca aaatcaaact cataagcggt acgaccaaac tctttaaagt
     2581 acttattgat tcgatttttc cattctatcc atacttcctg tgaaactcca acaggcacaa
     2641 ctgaagacat gaaatcttct gctatcttta ttgcagaact gttttgcaga taaaggttga
     2701 tgctgttatt ttgctttacc cattctgaaa taccccacaa ctccttttcc gattgcagaa
     2761 caatcgtatc aaacccaagc aggaaaacgg aagtatccgt tataccagca cgtcgaactg
     2821 ctccttgaaa taattttgta aataatgttt cactgataga agcagcaggc aatgtaagct
     2881 gaatccttgt gaaataaata catgcggcat aaaatattgt ctgaataccc tccataattt
     2941 gatgggcgtt catagtatgc atgggctttt cttcccattg tttaatagca tcctcatatc
     3001 cttttcgtgc cgtttcccag cgtgcaacgc tgttcataag tgtcctacgt aaagagcgtg
     3061 gggaaaggga tttgatagca atcaaaaatg gctttgattt agcagaaaga taaacatatc
     3121 cattaataac cgtatatgcc ccgttttttg gtaataattt tttcgcactt ttaccaaaca
     3181 tatctatcca taaaagctct gatgcacggt ttacgatttc aagaccaagc gtggcgaaca
     3241 aaggtgtaac aggatttggc aagtgttctg ccaaacttcc ctttgtgtat acaacatcct
     3301 gttctggcaa tacccattct ggtggcagaa cagtaattgg acgagcctga acaatgtaca
     3361 atttatcttt ttccagtgcc cactctacat ccataggcat ttgataatac ttctcaattt
     3421 tctttccaag ctgtatcagt tgcattacct gattacgagt aagagcatat ttttttctca
     3481 acctttcagg aaccatggtt tcttctgttc cttctgaagt acgaaccgtc ataatctctt
     3541 tatttgccac ttcatacgat ataattcgtt cggaatcctt atccaccacg attgtatcgg
     3601 gtgtaactaa gctagaaacc accgcttcac caagccccca tgcagcgtta ataatcattt
     3661 cgctacgtct accattgatt gggtttaacg taaacataat accagatgaa tcggaaaatg
     3721 caagcttttg tactacaaca gcaagtgcga caatctcgtg ctttatatta ttcttcacac
     3781 gataagcaat agcacgagct gtccataagg aagcccagca tctctttaca gcgtcaataa
     3841 ctttatcttc accttgtatg ttaagataag tttcctgctg acctgcaaaa gaagcgtcag
     3901 gtaaatcttc agcagtcgca gacgaacgga cagccaccga tatgttccct aatccggcat
     3961 aagccgtttt aattgcatcc gacacttcct gtggcatttc tccattatgg aaaagctccc
     4021 ctattttctt agatacatct tcaagttggc tggtattatt ggaatcaata ccatctagca
     4081 gtttattaat tcgagactga atatggttcg tctcaacaaa aatctggtat gaagcagtcg
     4141 taacatgaaa accgtctggc acagggatgc ctgctgtcaa tagttttgat aaagacatcc
     4201 cttttccacc aataatttca atggttgctt gtttatgttc cagtggcaag acataataaa
     4261 tcataatcat tcacctccat cattcataat tccgtgcaga aatatattca ttgtttcatc
     4321 caacattttt tccaaactat ccgatggtct tgacgtgtag ctgattgata acatggagtt
     4381 aattaacaaa cgtgtcacaa gagcggtatt cgttttgcga aagatgcctg tggtcacacc
     4441 attatcaatt accgatttta taatatcttg atatgcataa cgagcatctt taagtctctt
     4501 tcgatactct tcttctaaat aatcagattc tgtatttaac cacatcagta cagttctata
     4561 ctgctctgga aatctaagat gattaagcat gatttttcta aggcactgtt gtggtgaagg
     4621 ttcaccatca ataatcctat gaacttgttg gatagttttt tctatttctt tctcaactgc
     4681 atatacaaca atctcgtctt tcgttttgaa aaaatcgtat agacttgact ttcccatgtt
     4741 ggctaaaact gcaatttctc tcattgatgt tttttggaat ccattttcag ccatgagact
     4801 caaagcaaca gcaactattt tttctcggcg ttctgcttgt tgttcgggtg taagcacaat
     4861 tccttttggc atagggcgat acctcctttt agcgaccact ggtcgtttct tttttattta
     4921 tcatacagcg accaacggtc acttgtcaag gttaattttt ttcattgtat atggtactcg
     4981 agataaaaat gttcatttta gaaattgctc tatttaagta gaaataggct tcatcattag
     5041 aaaacaagac tataaatttg agatatttat catgttcaaa tttcatcaat atcaaagttt
     5101 aagatattaa taaaaaaaga attactaaga aataacatat aaagattcaa aaaaggagtt
     5161 gaacttaaaa ttcaattaca aaaaatcaaa tttaagacaa ctcctttatt ctttatctct
     5221 actattcttt atctctattt atttattaca actccctaac atttaccact actgccttag
     5281 gtctagtcat agcctcaatt gaatacttaa caccttgagt tcccatacca gatgccttta
     5341 cacctaaaaa tgggaagtgg tctggacctc tttcagtctt attatttatt tgaacagtac
     5401 caacttcaag tttatcagct atataaaatg ccttatcaat atcacttgta aatactgatg
     5461 attgaagccc atactctgat tgattagcaa tttcaattgc ttgatttata tctttaactc
     5521 ttataattgg aagtactgga ccaaatggtt cttcccaagc aattttcata tcaacattta
     5581 cattatctaa caatgttgga tagaataaat tattttttct aacattgcct gttattaact
     5641 ttgctccttt atgtaatgca tcatcaacca acccttgaac aaaatcagtt gctttattat
     5701 ctataagagg agtgataact acttcatcca ttggatttcc aattgtcaac ttctctacca
     5761 atggctttat ttttccgact aatttatccg ccacactctc ttgtactaaa attctcttta
     5821 ctgccgtaca tctttgtcct gaatatgaat acgctccaga tactatattc ttagctgcaa
     5881 aatctaagtc tgcatcttct aatactatag ccgcatcttt tccaccaagc tccaataata
     5941 atggtaccat tccagatatt ttagatatat gctgaccaac ttcagtacta ccagtaaagt
     6001 taataaaatt aattccttta tgcgttacaa cataatcccc tatctcacta cccctacctg
     6061 taatagtatt aagtactcca tctggtatcc cagcttttct cataatttct accacatgaa
     6121 gtgcgctaat agccccttgt gtagcaggtt ttaatacgac tgcatttcca ccaataagtg
     6181 ctggagcaat ctttgacatt gataaattta caggataatt aaaaggagat atagctaaaa
     6241 cagtacctaa tggaaccctt gaaacataag acatcttatt tcttgttcca ccagggaaat
     6301 tatctccact aatggcttct ccctccatat tttttcccac atcagctgtg tatcttaaaa
     6361 aatctgctgt tctctcaact tctgcccttg cagattttat gtctttggct atttcaagta
     6421 ccagtatatt agctatctca tctatatttt ctaacaataa atcagcagcc ttatgaaata
     6481 tattgcctct cttgtataca ggcatttcag cccatacttt tatactctct tttgtgtttt
     6541 ttataacctc atctacctca tgttttgtca tagattgtac tttcccaaca agagaattat
     6601 ctattggtga gtaaatttct attggctttc tcgaattaga ttctacccat tttccattta
     6661 ttaaattttt atatacattt tcttttgttt taagttcatt aaacatattt ttattcctct
     6721 ctctgatata actttgtgta tttaaattat agccatttat tcgttatgta aacatttctt
     6781 tttttcataa acgtttacct ataaaaaaat tttaagttaa cgtttttcta cattcttcta
     6841 ttttaataac atactcgctc caaatctata taggtcacac tttaatacta atatatcaat
     6901 ataccaatat accaatatac caatatacca atataccaat ataccaatat accaatataa
     6961 atattataaa cataaatcac ttgacaaatt tataagtaga caatattata tatctatgac
     7021 tcaaaatatt tttaaagtca tatatatcat atatagacag aaaggattga taaaaatggc
     7081 taaaagtttt acagaaatag aaaaagacaa tataagaaaa agactaattt cagaatgtga
     7141 aatcaactgg tctaaatatg gatacaaaaa aactaatatt gatgaacttt gttcaaaatc
     7201 aggaattgca aagggttctt tctatacatt ttttgattca aaagaaaagt tattttttga
     7261 agtattaaat ggaatccaaa acagacttat atcatcagta gatgaaattt taagtaacat
     7321 gcctccaaaa gaaggctttg caaaatcctt aaaaatgtta tatcgattat atgataaaaa
     7381 tccattttta tattcaccaa ataatcctga tttcatcagc ctaataaata aactacctca
     7441 agatatgttt ttacaacttg agtccaataa catacaaagt ttttacgact taattgagag
     7501 acacaatctc aagttgaaga tagaaaaaga aaaagttttt ggtgtttgta atgctctact
     7561 atcaagtctg ttggtaaaag aaaatattga atataactat attgaagtgt ttgactttat
     7621 ggtggataat cttgtagaac acatacttga ataacaattt aaaataattt ttctaagtat
     7681 ttttttatac taaataggat taatatgatt gaattaatgc aaaaaataaa caatataatt
     7741 tataaaataa atgataaact aaatatcaca caaaacttat agaccctaga gcaaacccat
     7801 tccactctac tctaagccat ctataatagt ttgtatattt ctaataatat tagatattaa
     7861 agtaagaaaa aaattcctta taataattgc tattatatca attattgtaa atgttttaat
     7921 aatcttagat aggtaggtac atatatggta aaatcctcga acacttttaa taaaaacttc
     7981 aacttgatga tattaggtca aataatctca ttgtttggtg catcaatact taaatttgct
     8041 ttatctttat acatacttga tataactggt aaagcagaaa tatttgctac tatactagca
     8101 gtatcttcaa tcccaattat agtattctcc cctattggtg gagctattgc agatatattc
     8161 aacagaagaa acttaatggt tatttttgat ttttcaagta gtcttacagt actaatctta
     8221 gcagtattct tattcaacaa taatggttca atattacttg taggaattat tatgaccata
     8281 ttatcagtca taagcacaat gtatcaacca actgttcagt caagtacacc ccttttagtt
     8341 gataatgagc atttaatgaa tgcaaatggt attgtagctg gagttgcatc tcttacaaat
     8401 attgcagtac ctgtattagg aggtgtcttg tatggtgtga ttggaattaa tgcaattata
     8461 gttataagtt gtgtaagttt ctttctatct gcaattatgg agatatttat acagattcct
     8521 tttatcaaac aagagcaaaa cagcaacatt ataaaaacta tatttactga tataaaggat
     8581 ggtattgttc atatatgtaa agaaaataga tttatactaa aaatactttt tttagctata
     8641 gcaataaact tatttttaag ctctatgtta atagttggta tcccctatat agtaaaaatc
     8701 actatgggag ctagtaactt cttatatggt ctagcagaag gagcaatttc ttttagttca
     8761 ctggtagctg ctgttagtat tggtattttc tcaaaacatc taaaaatcac taatttgcat
     8821 atttccttta ttatatgtgc agttatgttc ataccaatgg cggtttcagt atatccattt
     8881 atattaaaca caggatttcc agttccattt ttaatcttta tagtatcatc tatgctaata
     8941 ttttttacaa cgactcttat ctcaatatat gttataacag tcacacaatt aaaaactcca
     9001 aatgaactct taggaaaagt aatgtcaatt ctttttgcag gaacagcaat tgcaatgccc
     9061 cttggtcagg ttatgtatgg taaacttttt gatttattat cagatagagt gtacatcatt
     9121 atactaggag ttggatttat tacattactt atagcatttg tatcaaagat aacattgagg
     9181 aacgaaaatc tttagataca aagaatttag atataaagat ttaattttca ctaatttctg
     9241 aatttaaatg caaaattgtg tatcagtatg ttgacgtttt agaaattgat tgatataatc
     9301 aatggaaaat aaactttata aaaattcaaa atattcagct agaggagtga taaaaatgag
     9361 ttacatgcca actttagaac aagcatggga aatattaaga aaatacaata aggaagaatt
     9421 tcatataaga catgctcaaa ttgtatctgg agttatgaga tactatgcaa aggagtacga
     9481 ccctgaaaga gtagaatttt gggaaatagt tggactttta catgatttag attttgaaat
     9541 gtatcctgat gaacattgtg ttaaacaaag agaattaatg acagagcttg atttagataa
     9601 atcaataatc gattctacaa taagtcatgg atatggatta actggttcag atgttaaacc
     9661 agagcaattt atggagaaag tattatttgc tgtggatgaa cttactggtt taattggtgc
     9721 tgctgcaata atgcgtcctt caaagagcgt ttctgattta gaattaaaat ctgttaagaa
     9781 gaaatttaaa gataaatcat ttgctgctgg ctgttctcga gacgttataa gaagtggtgc
     9841 agaaatgctt ggatgggaac ttgataagct tatccaaaca actatagaag ctatgagaag
     9901 cttgattcca gagatggcaa tatagctcct ccatacatat accatagaag ttacatcttt
     9961 aaagagcatt tctctttttg atgcaacttc tttttatatt ttgtagaata aaaagtttaa
    10021 tattttaaac tcactatata ataaataata taatgtataa tagtcataaa ctttctaacc
    10081 ttaaaagttt ctaataatca ataaacaatt ctaatataca aatgtcatta tatgatttaa
    10141 ttatatatct tcaatttata tattaaaata aatatatata tattaaattc ttatacacca
    10201 aatttttaca ttaaacttct atgcaccaca tttatacata ttaaattctt atatattaaa
    10261 ttt
//
LOCUS       DAGSWI010000140        10239 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.141, whole genome shotgun sequence.
ACCESSION   DAGSWI010000140 DAGSWI010000000
VERSION     DAGSWI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.141"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            502..1011
                     /locus_tag="MJF80_003214"
     CDS             502..1011
                     /locus_tag="MJF80_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBZ0281852.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            1039..1233
                     /gene="rpmI"
                     /locus_tag="MJF80_003215"
     CDS             1039..1233
                     /gene="rpmI"
                     /locus_tag="MJF80_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBZ0281853.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1289..1645
                     /gene="rplT"
                     /locus_tag="MJF80_003216"
     CDS             1289..1645
                     /gene="rplT"
                     /locus_tag="MJF80_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBZ0281854.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            1955..2500
                     /locus_tag="MJF80_003217"
     CDS             1955..2500
                     /locus_tag="MJF80_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281855.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            2487..2660
                     /locus_tag="MJF80_003218"
     CDS             2487..2660
                     /locus_tag="MJF80_003218"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281856.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLLGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            2935..4860
                     /locus_tag="MJF80_003219"
     CDS             2935..4860
                     /locus_tag="MJF80_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBZ0281857.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            5261..7165
                     /locus_tag="MJF80_003220"
     CDS             5261..7165
                     /locus_tag="MJF80_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBZ0281858.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
     gene            7849..8628
                     /locus_tag="MJF80_003221"
     CDS             7849..8628
                     /locus_tag="MJF80_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBZ0281859.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(8938..9759)
                     /locus_tag="MJF80_003222"
     CDS             complement(8938..9759)
                     /locus_tag="MJF80_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBZ0281860.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEIWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
BASE COUNT         4186 a         1083 c         1747 g         3223 t
ORIGIN      
        1 ttagaatata atgtaggatt tgttatgtag tataatagta tattagagta caatatagaa
       61 tttgttatgt agtatagtag tatattagaa tacaatatag aatttgttgg gtagtatagt
      121 gttatattag aatacaatat aggatttgtt atgtagaata tatagagtca tgtagaatat
      181 acatatataa aaggattttg ggtataaatt taaaaattaa tagcaaaatg agtagtatag
      241 acatataatt agtagtagga agtaattaaa tttttcctaa acaaaaaaaa tagttgcaaa
      301 taatttgatt aagtgatata gtaaataagg taagaataaa acaaagaaga agtccgcttc
      361 tcaccttacg ccggaggtta gtaaggtaaa tataagttaa agcttaattt taatttaagt
      421 ttgtttaaat ttggtaactt atattgcgga tgattacgtc atccgctttt tattttataa
      481 aaattttgga ggtgtcctac aattagcaaa gaattagcaa tcaatgaaca aataaaagat
      541 aaggaaataa gagtcctttc accaacaggt gaacaacttg gagtaatgcc tactaaagaa
      601 gctcaagcaa tggctaatag caaaaactta gatttggttc aaatctcgcc gaatgcaaat
      661 ccaccagtat gtaagataat ggattatgga aagtttagat atgagcaagc gagaaaagag
      721 aaagaagcta agaaaaaaca aaaaactata gttgtaaaag aagtaagact tagaccaggt
      781 attgaacaaa atgatttaaa tacgaaagct aataatgcta taaagttcct taaaaaaggg
      841 gataaagtca aagttgaact tagatttaga ggaagagaat taggacataa agacataggt
      901 aaagaagtta tgcttaagtt tttagatata ataaaagagt ttggagaacc aacaaaagcc
      961 cctgcatttg agggtaataa tatggttgta attatagatc caaaaaaata gatgattaac
     1021 tgagaggagg aatttgtcat gcctaaaatg aaaactcata gaggtgcagc aaagagatta
     1081 aagaaaacag gaactggaaa gttaaagaga gctaaagctt ttaaaaaaca tatattaact
     1141 aaaaaatctg caaaaactaa aatgaactta agaaaatcaa ctttagtaag tgatggagat
     1201 gctaagagaa tagcacaatt attaccatac taagatacag attgtaaata ataactatag
     1261 aagagggata taaaaggagg tcataacgat ggcaagagtt aaaaaagcta tgaacgctcg
     1321 taaaaagcat aagaaaatat taaaacttgc aaaaggattc agaggatcta gaagcaaatt
     1381 atatagacca gcaaacacat tcgtaatgaa agcattaaaa aatgcttata taggaagaaa
     1441 gttaaagaag agagacttca gaaaactttg gatacaaaga ataaatgcag ctgctagaat
     1501 gaatggaata tcttattcaa gattaatgaa tggattaaaa ttatctggtg ttgaagttaa
     1561 cagaaaaatg ttatctgaaa tggctataca agacccagaa ggatttgcaa aattagcaga
     1621 agttgctaaa gcaaaattag cttagtattg aatcatgaga tgtaaagtct ttgagttata
     1681 taaataattc aaggactttt ttattttttg aaaaatactt attagtgtca tgtacaacca
     1741 attttataat gataaataaa ataagcaata caatagaatt ttatgagatt tattataaga
     1801 attgttacaa ttatttatga tagctataaa aaatcatagt aaaaatgata ctgatgtatg
     1861 ttgatatatg tataaagttt ccaatcaatc ttgaattaat attatcaaca tgataatatt
     1921 atcttattaa taatataaat aagggggaga taatatgaat gaaaaactaa tggattgttt
     1981 tgtaaaccca attaaatgca agttattgat tgaaattttt tcaaataatc gaactacagc
     2041 taaacaatta ggagaaaaat ttcaagatat atcaaaggca acactttatc gttaccttaa
     2101 taaaatgttg aaagatgata ttataaaagt agttgaagaa aataaaataa ggggaactgt
     2161 tgaaaaagta tatgctatga agttagatat agctgatgat gtgaacaaaa ttatagatga
     2221 gaactctgga gaagaatatg ctcaactgtt tactcaatat atgatgggat ttttaagaga
     2281 attttatgaa tacgctgaaa gaaaggaaaa ggatatccta aaagatggtt caggattttc
     2341 tgtctgtcca gtatatgcta gtattgatga gatagttgaa gtgagtaaaa aaattgctag
     2401 tatactagaa cctctaagaa ataactcgca cgaagaaggc agaagaatgc attctatagg
     2461 agtgataatc actccaccta aagaggatga agggaaataa atctgattta catgaggtag
     2521 agccaattta cacagaaaat cgttttatga tttacatatg tatccagagc aaagtatctc
     2581 tgcttggatt gtacttcatt gtaccatttc atccaatgac ttggagtggt acaatgaagt
     2641 tttttaattt ttcatattaa tgctaaagaa atgaacttta gaccacatat gcaattaggt
     2701 tgcattatac aattagacca catatacaaa ataaaaatca gtatttttat agcagataaa
     2761 ttgactgtca ttcttcttaa aaatcatcta ttagttattg ttagcatatt gaaagaaatt
     2821 atagtaatct attgaataaa tgaatattgc ctataatcat ataaactgct atagtttaaa
     2881 ataaagcagg acgttaaatt ttatagtaat ctaaaaattt aagaggggga ttttatgaaa
     2941 ataaaatttg ttattatgcc attggtgata tttttaatgg tattatttgt ggggtgtcaa
     3001 agtacaaaag atacagaagt taaagcaaaa gatacagaaa ttaaaatatt agggacaggt
     3061 gatttacatt caattttgac agactctatg gtttcgtatg taaatgagga aagggaaaag
     3121 aataaaaatc tgcttatggt agatgcggga gatttctatg gtacacaaag tagagaaatg
     3181 tgggaatggt cttctggaaa aaagttaatt aatattaaaa gaaatggtag agcagagtat
     3241 gatgacataa ttaagagtag taaagatgaa gtacctatag ttaaagatat ggcaaagttg
     3301 aaatatgatg ctgtagtttt gggagataat gaatttgttt caaatgataa gcaaagtctt
     3361 gataaactgg tttctgattt taaaaataat aatatgccat tggtatcagc aaatatatat
     3421 gaacaatctg gagaaaatta tgtacaacca tatgtaatga aaaatattaa gacagatgag
     3481 ggcaatgtaa aagttggaat actaggtctt actataaaag aagtagggga aacccttggt
     3541 cttgaggatt ttgaaaagga taaaaaagct agagagcttg gagaacaagc tgattacaaa
     3601 ggaaaattat atgcaaatga tttagtagaa gatgcaaaga aatggataaa agttatggaa
     3661 aaagaaagtc cagacataat agttgcagtt gtacactcag gggaagaacc taaaacagat
     3721 agaaatccag gaaatagaat aaaagagtta gcaactacag tggatggaat agatgcaatt
     3781 gtagctggtc atactcatga aaaaatagat gaacacaaat ataaaaataa atctggagat
     3841 gaagtaattg ttacacaacc aggttggcat ggagatagag tatcagagat taatttcaaa
     3901 ttaaataaag agaatggaaa gtgggacata aaggaaaagt ctagtaaatt aaaaataatt
     3961 gataaagaag taagtatagt tgctactgca gatttacatt cacatttctc tgataaatta
     4021 acagttgacc ttgttaatga acgtagtaac ccaattgaac ctgttgtagt agatgcaggc
     4081 gacttcttag accctcaaac agatgaaatg actcaatggt ataaagagtg gaaaagtata
     4141 agagataata atagtgataa aacgattagt agatgcccaa tggtcatgga tatgaatcag
     4201 ggaatgtatg atgcagtagt acttggaaat catgaatttg tatctgaaaa agatgaattt
     4261 tggtcagatg aacgcctttt agatgctgtt atagaggatt ttgaacaggt ggcaatttct
     4321 gtgttatcgg caaatatata taggcaatct ggtaaaaatt atgttaaacc atatattata
     4381 aaagatgtgg agacgagtga aggtaatgta aaagtaggaa tattgggact tactataaaa
     4441 gaagtaggta aaggtcttaa aaatgtaaac ttacaagagc aatttcaata taaggataaa
     4501 ttgtatgcaa atgatttagt ggaagatgca aagaaatggg taaaagttat gaaaaagaaa
     4561 aaaccagata taatagttgc agttgtacat tcaggagaag aaccaaaaaa tccaaaacat
     4621 ccaggaaata gaataaaaga actagcaact acagtagatg gaatagatgc aattgttgct
     4681 agtcatactc atgaaaaaat agatgagcat gaatataaaa ataagtctgg agaaaaagtt
     4741 atagtgacac aaccaggtga gcatggtaaa tattattcta aaattacctt taaagtaaat
     4801 aaagaaaagg gttcgtggat tattaatgat aaattcagta aaacaattga ggttgaataa
     4861 ctttataaaa tttatagata aagtaaatta tctgatattg acatgatata taagataaag
     4921 tttctaatat tagtataatg cataatgtga aatattttaa taataagaga ataaacaaga
     4981 caaataaaaa tattttaaca tgtaagatat agtatgaact attatactaa tgcttacttt
     5041 ataatttgaa catgttagaa taagtcttat ttgaaaattt tagtgtatta gagtaagtat
     5101 tattttacca gtaaaaaata ttgagaacta tatgtagatt tgcttaaaaa ggaatttaca
     5161 tatagttctt ttcgtataat aaatagtaca atcttatatg gtaaagataa agtgtgatat
     5221 taaattttat tatcatccaa aatctaagga ggagtctctt atgaaaataa aatttattat
     5281 agtagcatca ataatatttt taatagtatt acttgtgggg tgtcaaagta ctaaagacac
     5341 agaaatcaat gtactcgcca caagtgattt acattcaatt attccagata atttaatttc
     5401 ctatgtagaa gaggagagaa aatatgatga gaatctactt atggtagatg caggagattt
     5461 ttttgacatg caaagtggtg agatgaataa gtggtttact ggtcaaaaat tagtaagatt
     5521 taaagatggg ataccagagt acaaaaaaat agctgaacca cgtgaaggag aagttccatt
     5581 ggctaaaaaa atggcaaagc tgaagtatga tgcagttaca tttgggaatc atgaatttgt
     5641 atctaatgac aagcagagcc ttgataaatt ggtttctgat tttaaaaata acaatatacc
     5701 attagtatca gcaaacgtat atgaacaatc tggagaaaac tatgtaaagc catatgtaat
     5761 gaaaaatata aatactgaca agggaactgt aaaggttggt atacttggtc tgactataaa
     5821 agaagtaggg gaacgttcaa ggtttaaaag ttttgaacaa gataaaaagg ctagagaact
     5881 tggggaacaa cctcaataca aagggaaatt atatgcaaat gatttagtag aagatgcaaa
     5941 taagtgggta aaggttatgg aaaaaaaaga aaagccagat ataatagttg ccattgtaca
     6001 ttcaggtgaa gagccaaaaa atcctaaaaa tccaggaaat agaataaaag aactagcaac
     6061 aactgtagag ggaatagatg ctattgtagc aggtcataca catgaaaaaa tagaacagca
     6121 tacatataaa aataattctg gtgaagaagt aatagtaaca cagcctggag ctcatggtaa
     6181 tagtatatca aagattaatt ttaagttgga taagaagaat gataagtggt ttataaaaga
     6241 caaatatagt gaattaaagg tttttggaaa ggaagtaaat ataattacaa cttctggtct
     6301 gaatccaaaa ttttcagatg aaatggcagt taatttgttt aatgaaagag atagagatgt
     6361 tcaacctata tttatagata ctggagattt tttaaatact aatacaaaag aaatgactaa
     6421 gtgggctaaa gagtggcaat acaatgaaaa taaaggcatc tataaaaaaa taaatgaatg
     6481 gccaattgta tatcttggtt atgatgctgt agtacttggt agccatgaat ttacagagga
     6541 taaagagact tttgaggaaa aaaaatctac attggattat attatagaaa actttgaaga
     6601 aatgaagata ccagtattat cagctaatat atatgatgaa tctggagaaa attatgtaaa
     6661 accttatata attcataatg tagaaactag ggaaggaaat ataaaggttg gagtattagg
     6721 tcttactact actgataagg atgaagaaga cttaagtaac tcaagcttaa gcaaagactc
     6781 aaagacttcg aaaattcctg aagaaacagg aaagttatat actaataatc tagtaaaaga
     6841 tgcaagcgaa tgggtaaaag ttatgcaaaa ggaaagtcct gatgtgattg ttgcagttgt
     6901 acattcagat aataatagta ctctaaaaaa tccaagtagt gaagtcaaaa aacttgcaat
     6961 gtcagtagat ggaatagatg ctattgttgt aggtcatacg aaaaataagg tagaagaaca
     7021 tatttacaaa aataagtctg gggatgaggt tatagtaaca caatctggag aggattgtta
     7081 ttcaaaaata agtcttgaac taagaaaaga aaatggcaag tggaatatca taaacaaata
     7141 tagtaaagca ataaaaatga agtgaattta ttgatatata aaccattatt aatactgatt
     7201 ctacaatata attaacactg atttttataa tataataaat actgattcta taatataatt
     7261 aacactactt tttataatgt catcaacact gattctataa tataattaat actacttttt
     7321 taaatataat caatactgat tccataatat aattaacact acttttttaa atataatcaa
     7381 tactgattct acaatattat taatactact ttttataata taatcaacac tactttttac
     7441 aatataatca atactgattc tataatataa ttaacactac tttttaaata taatcaatat
     7501 cgattctata atattaacac tactttttaa atataatcaa tatcgattct ataatattaa
     7561 cactactttt tataatataa tcaatactga ttctataata taattaacac tactttttaa
     7621 atataatcaa taccgattct ataatataat taacactact ttttaaatat aatcaatacc
     7681 gattctataa tattaacact attttttatg ctatataaca attattgaaa atgatataaa
     7741 tataatatct caaataatat gaatataaca tctgaataga gggtgtttta tttgtaaaaa
     7801 ataagtttac agtttgagcg tataaactta tggtaaagga gaataattat gagttataag
     7861 aagattgcaa ttataacggg tgctaatagt ggttttggta aagagtttct aaagttatta
     7921 ataaatgaag aagaaataac agaaatatgg gcagttgcaa gaaacaaggc gagattaaat
     7981 caattagtag atgaatttgg aagtaaggta aaaatttttt caaaagattt atcaaaaata
     8041 gaagaagtaa aagaaattgg tacattttta agtaaagaaa atgtatgtat aaaatacctt
     8101 ataaacaatg ctggttttgc aaaattctgt tcatacaatg atttaagtat agatgaatct
     8161 attaatatga ttgatttaaa tattagtgct gttgtagcat tgggacttat atgtattcca
     8221 tatatgcaaa agggaagcca tatcatgaat atttcttcac aagcatcatt ccagccattg
     8281 ccatatcaaa atatttatag ttcaactaaa gcgtttataa gaaattatac aagagcatta
     8341 aatgttgaac ttaagagtaa agggataaat gcaattgctg tatgtccagg ttggatgagt
     8401 acaaatctat ttaagagagg tatagttgta gctgaaaaag ggacaaaaaa tttcccaggc
     8461 atagtgtctc cagacattgt tgccaaaaaa gcattgaagg atgcaaagaa aaataaggat
     8521 atttctgtgt atggtataaa tacaaaatcc agtcatttat tagcaaaact attgccacaa
     8581 aaaatgatga tgaaggtttg gttaatgcaa caacatatta aggaataaga gtttcggtaa
     8641 ttaagtatca gttggagatt caatctgtta tatattatga gaagttttgt tgagcaaata
     8701 ataattgtga caaaacggct tatctagtgc cgataagccg tctataatgt atataaaata
     8761 tatttttgat tttactttat ttagatattg aagtattaaa atatgtagga cttgaaattt
     8821 tataaagata aatactatga tattttgtaa aaactgagat atgttgatat tacatagttt
     8881 aactatactg ttaaattgaa gttttcttat tttgcattat gcttattact taatgattta
     8941 gactataatt tccataccat catatgcaaa ataaatgtta tcaagtttct tttctaattc
     9001 actgtaataa ccatatgatt ttccccatat ttcgtcaata tgtgtaatga taatcttttt
     9061 aattctataa tagtttttaa tttcaacaat ctcatctaaa gtaaatattt catctttaaa
     9121 tggtgtgtct ttaagttttg taccatcatt taatatacca tcatcagaaa ctagacttat
     9181 tattaaaaca tcagcatcaa agtacatatc actatgagta aatggcttgg gattacagca
     9241 ggcatatatg actttttttt cattctcttt gataacatag aaggttatat tcctgtctgg
     9301 gttattgtta atcaaatcaa tatcaaaatt atttatcttt acatgcgaag tttcattaat
     9361 agaaatgcaa tttataacat caccataata tttaagacaa tcgagattcg ttcggtttat
     9421 atcttcaatt acttcaggaa gcgcataaaa gccaattgga ttattttctc ctgtgataaa
     9481 gtcacatcct attttttcga caattctaat acccttaaca tggtctgggt catggtgaga
     9541 aattgaaata tgttttactt ccgatatttt ttgtctgtta caagctactg caatgtcttc
     9601 aggtgtgtct atcagcattt ttatatcatc aatatataaa cttggcccta atcgttcata
     9661 tcttccgcct ttttgtctgg cttcttcaca tattttgcat ttgcaaaatg aacttggaat
     9721 aattgacata ccgccactac caacaatttt taatttcaaa gttattacac caccttctta
     9781 aaatattaga ttatataaat aatatacaag cacaactgat taacaaaatt atgagttaat
     9841 aaattatatc acatgcaaat atgaaagaat agtatgaaat ttttataaac atatattaaa
     9901 tgtttctcaa tgaaattgac tttgtatatg aactgaatga cttattcaat atggataaga
     9961 catttttctt gtaataagat atgattgtcc tataaggttt atgtaatttt gatataattt
    10021 gatataatat attgattaac aacatacatg ttattggtga gcatatttct atagaatgga
    10081 atatataatt ttttatattt atcattgtat tattgtacaa agaaagttaa aaagagtaga
    10141 gtacaaagga attatttgta ctttactctt ttattgtttt gttaaaaaaa ctaatttata
    10201 ttagtgatta aaatatattt aactagagta taaggatag
//
LOCUS       DAGSWI010000141        10238 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.142, whole genome shotgun sequence.
ACCESSION   DAGSWI010000141 DAGSWI010000000
VERSION     DAGSWI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.142"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            92..385
                     /locus_tag="MJF80_003223"
     CDS             92..385
                     /locus_tag="MJF80_003223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281861.1"
                     /translation="MEEYIKNNNPIPNSYKKDKIFINTLKYSKDKTDLPFWYHIRRVR
                     MSDETLKKLSAKADISIKTLKSIQNKNLTLVRTADVAKRKELTFILGGEGITI"
     gene            474..977
                     /locus_tag="MJF80_003224"
     CDS             474..977
                     /locus_tag="MJF80_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBZ0281862.1"
                     /translation="MEEMRVYIANLGKYNEGELVGDWFTPPIDWDDLKERIGLNSEYE
                     EYAIHDWELPFEISEYTSIEEINRLYSMAEELASTDMGEAISDIQKAFFSSFEELYEH
                     KDDIVYYPGCSDMSDVAYYLIDDCNMLGEISENVRNYINYEAYARDLEINYSFVVTNH
                     GVFEYIN"
     gene            1294..2181
                     /locus_tag="MJF80_003225"
     CDS             1294..2181
                     /locus_tag="MJF80_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281863.1"
                     /translation="MKKNENVEITKQAYSILENGYALQKSSCCLQGSFFEGVSDEDYK
                     KIDYKLKAIRQDFAKILVIMNSIDRTYSLYQKDEYVPSYISIMGNQATNEIGCFIEYL
                     YAKYRVILEYFQQILEICIPPRLSDPQSEQYNKLKKSHKKYNFLLKYLAENIDETNNI
                     LNMEWFQHIRVERDFIIHDGATCLVFGDKENLLFKVMTTDALDREYDNKQDEFFSNEK
                     GLIYYNRYWGVQISKLIVFSETIFRFLISISNMSEENKFILEDLFPQERNKFIDSYGT
                     EYNDVQDVLINMLRKIIEI"
     gene            2362..2766
                     /locus_tag="MJF80_003226"
     CDS             2362..2766
                     /locus_tag="MJF80_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBZ0281864.1"
                     /translation="MKKIKSYTGIWNVEKVIYAINDFQLPFPVTFTQMTWFVLTLFII
                     ILFGKLPPLYMIEGVFLKYLGIPVFITWFMSQKTFDGKKPYGFLKSIILYSLRSKITY
                     SGKSIKFKNKIIDENITIVRSELYVPDKIYRK"
     gene            2738..5185
                     /locus_tag="MJF80_003227"
     CDS             2738..5185
                     /locus_tag="MJF80_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0281865.1"
                     /translation="MFPIKYIENNLVFNKEGECFAYYELIPYNYSFLSSEQKFIVHDN
                     FRQLIAQNRNGKIHALQIATESSIRSTQERSKKNITGRLKEIAYEKIDEQTEALVSMI
                     GDNQIDYRFFIGFKLLVTEEEMNIKNIKKSILANLSDFIYDVNNKLMGDFISINNDEI
                     MRFSKMEKLLENKISRRFKVRRLDKNDFGYLIEHNYGKKGTVFEDYKYHLPTKKLKRE
                     TLVKKYDLIKPTRCLIEEKQKYLKIIHEDSESFVAYFTIESIIGEMDFPSSEIFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALTTVRNKKKELKDLDNHAWQAGNETGTNIVEALDAVN
                     ELENNLDQTKESMYKLSYVIRVSADSIDELKRRCDEVKDFYDDLNVKVVRPMGDMIGF
                     HNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQQLGENEGIYIGYSLDTGRNVYLKPS
                     LASQGVKGSVTNALAAAFLGSLGGGKSFANNLIVYYAVLFGGQAVLVDPKAERGNWKE
                     DLPEIAHEINIVNLTSDIENKGMLDPYVIMKEVKDSESLAIDILTFLTGISSRDGEKF
                     PILRKAIRNVTQSKCRGLLKVIDELRKDNSQISNSIADHIESFTDYDFAQLLFSDGNI
                     KGSINLEKQMNIIQVEDLVLPDKDTSFEEYTTMESLSVAMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWTFLNVAQGKTLSNKLVRAGRAMNAGVYFVTQNAGDVSDENLKNNIGLK
                     FAFRSTDINEIKRTLEFFGVDKEDDNNQKRLRELENGQCLIQDLYGRVGVIQIHPVFQ
                     ELLTAFDTRPPIRKEVD"
     gene            5185..7401
                     /locus_tag="MJF80_003228"
     CDS             5185..7401
                     /locus_tag="MJF80_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0281866.1"
                     /translation="MKSKVFRNISWMIILIVILVLFFIYISESIVYAEGLVDETVNSN
                     NVYSMYPLRNYQLDFYVDNSWGWLPWNWTDGIGKSVQYGLYCITNFIWIISLYLSNAT
                     GYVVQEAYKLDFINDMADSIGKSIQTIAGINQKGISNNGFYIGFLLLFILILGIYVAY
                     IGLIKKETSKAINSVINFVVIFIISASFIAYAPEYIKKVNDFSSDISTAALNLGTKIV
                     TSNSNSEGKDSINLIRDNLFSIQVQQPWMLLQYGNSNIEEIGKERVEKLISTDPGEDD
                     GKKREEIVKTEIEEKHNNNLTITEVMSRLGKVFFLIIFNIGITIFIFLLVSMMIFSQI
                     LFIIFSIFLPLSFLLSMIPSYESIGRQAVVKLFNTIMMRAGITLIITVAFSISTMFYT
                     ISKDYPFFMISFLQIVTFAGIYMKLGDIMGMFSLHSRDSQNMSSRIFRKPYRKMQNKS
                     KHIERYIRQALISRGKDKNKLSFNRSNKGNINNLQKRVDYLPSRDDYNKSINKESFLK
                     HMGNNIGAVIDTKKNIADKSRQVKENIKDIPIQTKYAFHNTKEKTKDNISDFKRGIVE
                     EKEKRQQGRDKKQQNHRETIAQKRAKLGNVVVSKDKNSKDNKNIDNYSNLKDKPSIGS
                     ENVKKKKGENIRPTVSEKIVNDKNLEKSNSINKIEMPTEEILHKKRDITNQGKESSNK
                     VKNNQNEKFKNSKAIKIERHNINSNSKDSRYNHINSKERKIKNKPNFTPENKVRKQ"
     gene            7398..8399
                     /locus_tag="MJF80_003229"
     CDS             7398..8399
                     /locus_tag="MJF80_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBZ0281867.1"
                     /translation="MKIKISIIFISIFLCIFGVILLFIPLISDENFNQDRNQNIIGMN
                     LSPEVLKYKPIVERYAQKYDIKEYINYLLAIMQVESGGILVDVMQSSESLGLSPNTLG
                     TEESIEQGCRYFSNLLKTSKEKGCDLNSTIQAYNYGFGYLNYIQSNGKVHNYNLAERF
                     SQNMAQGVKVTYTNPIAIAKNGGWRYKYGNQFYVELVLQYIGTSEFSNETVQIIINEA
                     LKYKGWKYVFGGDNPNTSFDCSGLTQWCFKKAGINLPRTAQEQYDIMQHITLDQAKPG
                     DLVFFHGTYNAGTYVTHVGIYVGNNKMYHAGDPIGYADLTGSYWQKHLIGSGRIKNR"
     gene            8409..9302
                     /locus_tag="MJF80_003230"
     CDS             8409..9302
                     /locus_tag="MJF80_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBZ0281868.1"
                     /translation="MLRKKDKKHKVNKISVIRLGINRKSVVVLWIVLIVSIVFGVYKN
                     FTAIDKHTIHEKEIIEQKIIDTNGIENFVKNFAKEYYSWENNKESIERRTSKINEYLI
                     KELQELNRDTIRNDIPTSSSVKDIKIWSVNQNKENELDVKYSVEQTIKEGEDVKDVIS
                     SYEVVVRRDNSGNMIIIKNPTLSQMPEKLNYEPKKEETDSTIDQKTIKEITEFLNTFF
                     KLYPNASKNELEYYVKEDVLKPINKEYIFSELVNPIFTREEKNIKVSTSVKYIDQQTK
                     AIQISQYNLTLEKDGNWIIRK"
     gene            complement(9622..10023)
                     /locus_tag="MJF80_003231"
     CDS             complement(9622..10023)
                     /locus_tag="MJF80_003231"
                     /inference="COORDINATES: protein motif:HMM:NF013412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBZ0281869.1"
                     /translation="MENIMSFLGDANTPIAVSNIHNGKPQVRFISFKMIEGGKLYFLT
                     SKKKDFFNQLVENNNIQICSMPNENNELLRITAKVDFIEDLDLNKKAFEILPLLQKVY
                     ETPANKDIILLSISYQNIVKYSLSEILYCWL"
BASE COUNT         4213 a         1009 c         1636 g         3380 t
ORIGIN      
        1 taatatacat aatgaatacc ttaaagaaat tccacaggaa atattaaatc aaatattaga
       61 aaataatcat ttagttgaat tctatagtta tatggaggaa tatattaaaa ataataatcc
      121 tataccgaat tcatataaaa aagataaaat atttataaat acattgaaat attcaaaaga
      181 caaaacagat ttgccttttt ggtatcatat tcgtagagta aggatgtcag atgaaacatt
      241 aaaaaaatta agtgctaaag cagatatttc tataaaaact ttaaaatcaa tacagaataa
      301 aaacttaact cttgttagaa cggcagatgt agctaaaaga aaagagttaa cctttatatt
      361 aggaggagaa ggtataacaa tttaaaatta gaaattataa attttgcagt aaatcttata
      421 tagatttgct gcttttttat tatctaaaat aaaaaaggat gtgaaataat attatggaag
      481 agatgagagt atatattgca aacttaggga aatacaatga aggtgaatta gttggagatt
      541 ggtttacacc accaattgat tgggatgatt tgaaggaacg tataggatta aatagtgaat
      601 atgaagaata tgccatacat gattgggagt taccttttga aattagtgaa tacacttcaa
      661 tagaagaaat taatcgttta tattctatgg cagaagaatt agcatctaca gatatgggag
      721 aagcaataag tgatattcaa aaagcttttt tctctagttt tgaagaatta tatgaacata
      781 aggatgatat tgtttattat ccagggtgta gtgatatgtc tgatgttgca tattatctta
      841 tagatgattg caatatgtta ggagaaatat ctgaaaacgt gagaaactat attaattatg
      901 aagcatatgc acgtgactta gagattaatt atagctttgt agttactaac catggagtat
      961 ttgagtatat taattaaatt ttaatagatt ttataaaaga cagttaaata tagctgtctt
     1021 ttatatttta aagaaagctt atgttttaat taagtgtttt ttgtatgagt ataagtatat
     1081 ttagtaaata ttttagattt attagcaaag taaaagatgt tataaaatag agtaatttaa
     1141 aaatataatg ttaagtttaa tttggtattt atggaattat aataaataat tcatgtataa
     1201 taaaaaatat atattattga ataaagctag taaaagtcta gttagtttga ttttaaaact
     1261 acttgataaa tagtaatttt aggaggtaat tttatgaaaa aaaatgaaaa tgtagaaatt
     1321 acaaaacaag catattcaat tttggaaaat gggtatgctt tgcaaaaaag ttcatgctgt
     1381 ttacaaggtt cattttttga aggagtttcc gatgaggatt ataagaaaat agactataaa
     1441 ttaaaagcaa taaggcaaga ttttgctaaa attttagtaa ttatgaattc cattgataga
     1501 acatattcat tatatcagaa agatgaatat gttccatcat atatttctat tatgggtaac
     1561 caagcaacta atgaaattgg atgttttatt gaatacttat atgcaaagta tcgtgtaata
     1621 ttggagtatt ttcagcaaat actagaaata tgtattccac ctaggttaag tgatccccaa
     1681 agtgaacaat ataataagtt gaaaaagtca cataaaaaat ataattttct attgaagtac
     1741 cttgctgaaa atattgatga aacaaataat attctgaata tggaatggtt tcaacatatt
     1801 agagtagaaa gagattttat aatacatgat ggtgctactt gtttggtatt tggtgataag
     1861 gaaaatttat tatttaaggt tatgactact gacgcattag atagagaata tgataataag
     1921 caagatgagt ttttttcaaa tgaaaaggga ttgatatact ataatcgtta ttggggagtg
     1981 caaatttcta agttaattgt tttttccgaa actatattta gatttttaat aagcatcagc
     2041 aatatgtcgg aagaaaataa atttatatta gaagatttat ttcctcaaga aagaaataaa
     2101 tttattgata gttatggtac agaatacaat gatgtacaag acgttttgat aaatatgcta
     2161 agaaaaataa ttgaaattta aaataaataa ggattataaa tatatgtatt agactaataa
     2221 attccaattg agtgattaga atttgaagaa caatcttaat atattgcgaa ttaaaagtgg
     2281 acaattagtt tgttcacttt ttttatgttc aatatcaaca ttagtttaaa acaaagccca
     2341 aataaagaag gaggaatatg cttgaaaaag attaagtcat atacaggtat ttggaatgta
     2401 gagaaggtta tatatgcaat aaatgatttt caacttccat ttccagttac ttttacacaa
     2461 atgacatggt ttgtattaac actatttata atcatattat ttggtaagtt acctccatta
     2521 tatatgattg aaggagtatt tttgaagtat cttggaatac cagtttttat aacttggttt
     2581 atgtctcaaa agacttttga tggaaagaaa ccatatggat ttttaaagtc aataatacta
     2641 tattcactaa gatctaaaat aacatactca ggaaaaagta ttaaatttaa aaataaaatt
     2701 attgatgaaa atataactat agtaaggagt gaattatatg ttcccgataa aatatataga
     2761 aaataatctt gtatttaata aggagggaga atgttttgct tattatgaat taataccata
     2821 taactattca ttcttatcat cagaacaaaa atttattgta cacgataatt ttaggcaatt
     2881 aatagcacaa aatagaaatg gaaaaattca tgcacttcaa atagccactg aaagttctat
     2941 aagatctaca caagaaaggt caaagaaaaa tattacagga agattaaaag aaattgctta
     3001 tgaaaaaata gatgagcaaa cagaagcact tgtttctatg attggcgata atcaaattga
     3061 ttatcgtttt tttattggct ttaaactttt agttacagaa gaagaaatga atataaagaa
     3121 tataaaaaag tcaatattag ctaatttatc agattttata tatgatgtta ataataaatt
     3181 gatgggtgac tttatcagta taaataatga tgaaattatg agattttcaa aaatggaaaa
     3241 gttattagaa aataaaattt caagaagatt caaagttaga aggttagata aaaatgattt
     3301 tggctattta attgaacata attatggaaa gaaaggtaca gtttttgaag attacaaata
     3361 tcatttacct acaaagaaac ttaaaagaga aacattagta aagaaatacg atcttataaa
     3421 gccaactaga tgtcttatag aagagaagca aaaatactta aaaattatac atgaggattc
     3481 agaaagtttt gtagcttatt ttactattga aagtattatt ggagaaatgg attttccatc
     3541 tagtgagata ttctattatc agcaacagca gtttacattt cctattgata ctagtatgaa
     3601 tgttgaaatt gtaacaaata aaaaagcttt aactacagtt agaaataaga aaaaagaatt
     3661 aaaagattta gataatcatg cttggcaggc cggaaatgaa acaggaacaa atatagtaga
     3721 agcattagat gctgtaaatg agcttgaaaa taatttagat caaacaaaag aatcaatgta
     3781 taagttaagt tatgtaatta gagtatcagc agatagcatt gatgaattaa aacgtcgttg
     3841 tgatgaggtt aaggattttt atgatgatct aaatgtaaag gtagtacgtc ccatgggaga
     3901 tatgatagga tttcataatg aatttatacc ttcaagtaaa agatatataa atgattatat
     3961 tcaatatgta actagtgatt ttttagcagg attgggcttt ggggcaactc aacaacttgg
     4021 agaaaatgaa ggaatatata ttggatatag tttagatacc ggaagaaacg tatatctgaa
     4081 accatctctt gcaagccaag gagtaaaagg ttctgtaact aatgcattgg cagcagcatt
     4141 tttaggttca ttaggagggg gaaagtcatt tgctaataat ttaattgttt actatgcagt
     4201 attgtttgga ggacaagctg ttttggtaga tccaaaagct gaacgtggta attggaagga
     4261 agacctacca gagatagctc atgaaataaa cattgttaat ttgacaagtg atatagaaaa
     4321 taaaggaatg ttagatccat atgtaattat gaaagaagta aaagatagtg aaagtttagc
     4381 tattgatata ttaacttttc ttacaggaat aagtagtcgt gatggagaga agtttcctat
     4441 acttagaaaa gcaatcagaa atgttactca gagtaaatgt agaggacttt taaaagtaat
     4501 agatgagtta aggaaagata attctcaaat atcgaatagc attgcagatc atattgaaag
     4561 ttttacagat tatgattttg cacaattact tttctcagat ggaaatatta aaggttcaat
     4621 taatctagaa aaacaaatga atataataca agttgaggat cttgttttac cggataaaga
     4681 tacttctttt gaagaatata caacaatgga aagtctttca gtagctatgt tgatagtaat
     4741 tagtaccttt gcattagatt ttattcattc agatagaagt atttttaaaa tagtagattt
     4801 agatgaagct tggacatttc ttaatgtagc acaaggaaaa actttatcta ataaattagt
     4861 tagagcagga cgtgctatga atgcaggagt gtattttgta actcaaaatg caggtgatgt
     4921 atcagatgaa aatctaaaaa ataatattgg attaaaattt gcatttagaa gtacagatat
     4981 taatgaaatt aagagaacac ttgaattttt tggagttgat aaggaagatg ataataatca
     5041 aaaaaggtta agagagttag agaatggaca gtgtttaata caagacttat atggaagagt
     5101 tggagtaata caaatacatc cagtttttca agaacttctt actgcatttg atacaagacc
     5161 acctataaga aaagaggtgg attaatgaaa tcgaaggtat ttagaaatat aagttggatg
     5221 attattttaa tagtaatact tgtattattt tttatctata taagtgaatc tatagtttat
     5281 gcagaaggac tagtagatga aactgtaaat tcaaataatg tatattcaat gtatccttta
     5341 agaaattatc agcttgattt ttatgttgat aattcttggg gatggcttcc ttggaattgg
     5401 actgatggaa taggcaaatc agtacagtat gggttatatt gcataacaaa ctttatttgg
     5461 ataataagtt tatatctatc aaatgcaact ggatatgttg ttcaagaagc atataaatta
     5521 gattttatta atgacatggc agattcaata ggtaagagca ttcaaacaat tgctggcatc
     5581 aatcaaaaag gaatatctaa taatggattt tatataggtt ttctactttt gtttattttg
     5641 attcttggaa tttacgtagc atatatagga ttgattaaaa aagaaactag taaggcaatt
     5701 aattctgtta ttaatttcgt tgtgatattt ataatatctg catcgtttat tgcttatgct
     5761 cctgagtata ttaaaaaagt aaatgatttt tcttccgata ttagcacagc tgctttgaat
     5821 ttaggaacga aaattgtaac ttctaattca aatagtgaag gaaaagatag tataaatcta
     5881 attagagata atttgttttc tatacaagta caacaaccat ggatgttatt acaatatgga
     5941 aattctaaca tagaagaaat aggaaaagaa agagtagaaa aattaatttc gacagatcca
     6001 ggtgaagatg atggaaaaaa acgagaggaa atagttaaaa ctgaaataga agagaaacat
     6061 aataataatt tgactataac agaagttatg agtagattag gcaaggtgtt ttttctaatt
     6121 atatttaata taggaataac tatttttata tttttattag tatctatgat gatattttca
     6181 cagatacttt ttattatttt ttctatattt ttgccattaa gttttttact atctatgata
     6241 ccctcatatg aaagcattgg aagacaagca gttgtaaagt tatttaatac tatcatgatg
     6301 agagctggaa taactcttat tataactgta gcatttagta tatcaacaat gttttatacc
     6361 atatctaaag attatccatt ttttatgatt tcatttttgc agatagtaac atttgcaggt
     6421 atttatatga agttaggcga tataatgggg atgtttagct tacactcaag ggatagtcaa
     6481 aatatgagta gccgtatctt tagaaagcca tatagaaaga tgcaaaataa gagtaaacat
     6541 atagaacgtt atataagaca agctttaata tctagaggca aagacaaaaa taaactatca
     6601 tttaacagaa gtaataaagg taatataaat aacttacaaa aaagagtaga ttatttacct
     6661 agtagagatg attataataa atcaattaat aaagaaagtt tcttaaaaca tatgggtaat
     6721 aatattggtg cagttataga tacaaagaaa aatatagctg ataaatcaag acaagttaag
     6781 gaaaatataa aagatatacc tatacagact aaatatgcct ttcataatac aaaagaaaaa
     6841 actaaagata atatatctga ttttaaaaga ggtatcgtag aagaaaagga aaagcgtcaa
     6901 caaggtagag ataaaaagca gcaaaatcat agggaaacta tagctcaaaa aagagctaaa
     6961 ttagggaatg tagtagtatc taaagataaa aattctaaag ataataaaaa tatagataac
     7021 tactcaaatt taaaagataa accttcaatt ggttctgaaa atgtgaaaaa gaaaaaagga
     7081 gaaaatatta gaccaacagt ttcagaaaaa atagttaatg ataagaattt agaaaaaagt
     7141 aattctataa ataaaataga aatgcctaca gaagaaattc ttcataaaaa aagagatatt
     7201 acaaatcaag gtaaagagtc atctaataaa gttaaaaata atcaaaatga aaaatttaaa
     7261 aacagtaaag ctattaaaat agaacgacat aacatcaatt ctaattctaa agattctagg
     7321 tataatcaca taaatagtaa agagagaaaa ataaaaaata aacctaattt tactcctgag
     7381 aataaggtga gaaaacaatg aaaataaaga tatctattat ttttattagt atattcttat
     7441 gtatctttgg agtaatttta ctgtttatcc ctttaatatc agatgaaaat tttaatcaag
     7501 atagaaatca aaatataata ggaatgaact tatcaccaga agttttaaag tataagccta
     7561 ttgttgagag atatgctcag aaatatgata ttaaagagta tataaattac ttacttgcta
     7621 ttatgcaagt tgaatcaggt ggaattttag ttgatgttat gcaatcttca gaatcactag
     7681 gcctttcacc taatacctta ggaacagaag aatcaataga acaaggatgt agatattttt
     7741 caaatctttt aaaaacctca aaagaaaaag gctgtgatct taattcaact atacaagcat
     7801 ataactatgg atttggatat ttaaattata ttcaaagtaa tggaaaagtt cataattata
     7861 atttagcaga aagattttca caaaatatgg cccagggagt taaggtaact tatactaatc
     7921 caatagctat tgctaaaaat ggtggttgga gatataaata tgggaatcaa ttttatgtag
     7981 aattagtact tcagtatatt ggaacaagtg aatttagtaa tgaaacagtt caaataatca
     8041 taaatgaagc attaaagtat aaagggtgga aatatgtatt tggaggagat aatccaaata
     8101 cttcttttga ttgtagcggt ttaacacaat ggtgttttaa aaaagcagga ataaatttac
     8161 caagaacagc acaagagcaa tatgatatta tgcaacatat tacactagat caagcaaagc
     8221 caggagatct agtgtttttt catgggacat ataatgctgg aacttatgta actcatgttg
     8281 gaatatatgt tggaaataac aaaatgtatc atgcaggaga tcctattggg tatgcagatt
     8341 taaccgggag ctattggcag aaacatttaa ttggatcagg aagaataaaa aatagatgag
     8401 gtgaaaatat gttaagaaaa aaggataaaa aacataaagt aaataagata tcagttataa
     8461 gattaggtat aaatagaaag tctgtagttg ttttatggat tgttttaatt gtaagtatag
     8521 tgtttggtgt ttataaaaat tttactgcaa tagataaaca tactattcat gaaaaagaga
     8581 ttatagaaca gaaaattata gatacaaatg gtattgaaaa ttttgtgaaa aactttgcta
     8641 aagaatatta ttcatgggaa aataacaaag aatctattga aagaagaaca tctaaaatta
     8701 atgaatatct cataaaagaa ttgcaggaat taaatagaga tacaataaga aatgacattc
     8761 caacaagctc tagtgtaaaa gatattaaga tatggagtgt aaaccagaat aaagaaaatg
     8821 aacttgatgt taagtattct gtggagcaga ctataaaaga gggagaagat gtaaaagatg
     8881 ttatatcttc atatgaagtt gtagttagaa gagataattc tggaaatatg ataattatta
     8941 aaaatcctac attatctcaa atgccagaaa aattaaatta tgaacctaag aaagaagaaa
     9001 cagatagtac aatagatcaa aaaacaatta aagaaattac agaattttta aatacattct
     9061 ttaaactata tccaaatgca tctaaaaatg agttagaata ctatgtaaaa gaagatgtac
     9121 ttaaacctat aaataaagaa tatatatttt cagaattagt aaatcctatt ttcacaagag
     9181 aagagaaaaa tataaaagta tcaactagtg taaaatacat agatcaacag acaaaagcaa
     9241 ttcaaatctc tcaatacaat ttaactttag agaaggatgg taattggatt attagaaaat
     9301 aaaaaattac tatatataaa taataaaaat aaactgttta gattaaaagt tttggagttc
     9361 aaaacaaaaa gagataatct gtagtaatag aaaaggagtg gttaatgcca ctcctaaaaa
     9421 gctagtttca aactatatgg tctgcacttt acacgctata taatttggtc tacatttttg
     9481 tacaataaaa ttctaataat aattttatat tttctaattt agttaagtta attaagtatt
     9541 tttttattaa atgttacctt aatctttaag taaaacttct ttttcgtcag atagaagaat
     9601 actttacgat attcagataa tttatagcca acaatataat atttcagata aagaatattt
     9661 tacgatattc tgatagctta tagacaacaa tataatatct ttatttgctg gtgtttcata
     9721 aactttttgt aacaatggta aaatttcgaa tgccttttta tttaagtcta agtcctctat
     9781 aaaatctacc tttgcagtta ttcttaacaa ctcattattt tcattaggca tagaacaaat
     9841 ttgtatatta ttgttttcaa ctaattgatt aaaaaaatct tttttcttag atgttaaaaa
     9901 atataatttt ccaccttcaa tcattttaaa tgatataaac ctaacctgtg gcttaccatt
     9961 atgtatatta gaaactgcaa taggtgtatt tgcatctcct aaaaaactca ttatattctc
    10021 catattatta tacctccaac tcaaataact aataatttat tcaatatgaa aatttaaaat
    10081 tttgattgat aataaaaaaa gccatatcat aaaaaatatg actttttgag attatatagc
    10141 tttaaaattg tgaaatttat gagatatact tcatcaactg atatatattt aaatttgttc
    10201 caagtatcag agaaattttt attatttatt ttaacttt
//
LOCUS       DAGSWI010000142        10179 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.143, whole genome shotgun sequence.
ACCESSION   DAGSWI010000142 DAGSWI010000000
VERSION     DAGSWI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10179
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.143"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1432)
                     /locus_tag="MJF80_003232"
     CDS             complement(<1..1432)
                     /locus_tag="MJF80_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBZ0281870.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGINKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYN"
     gene            1713..3890
                     /locus_tag="MJF80_003233"
     CDS             1713..3890
                     /locus_tag="MJF80_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBZ0281871.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYKQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            3904..4326
                     /locus_tag="MJF80_003234"
     CDS             3904..4326
                     /locus_tag="MJF80_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBZ0281872.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            4734..5537
                     /locus_tag="MJF80_003235"
     CDS             4734..5537
                     /locus_tag="MJF80_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBZ0281873.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            5530..6198
                     /locus_tag="MJF80_003236"
     CDS             5530..6198
                     /locus_tag="MJF80_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBZ0281874.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            6434..7096
                     /locus_tag="MJF80_003237"
     CDS             6434..7096
                     /locus_tag="MJF80_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBZ0281875.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAIGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            7156..7953
                     /locus_tag="MJF80_003238"
     CDS             7156..7953
                     /locus_tag="MJF80_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBZ0281876.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            8071..9261
                     /locus_tag="MJF80_003239"
     CDS             8071..9261
                     /locus_tag="MJF80_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBZ0281877.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            9370..10119
                     /locus_tag="MJF80_003240"
     CDS             9370..10119
                     /locus_tag="MJF80_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281878.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         3953 a         1084 c         1759 g         3383 t
ORIGIN      
        1 cgttatatat ttttttcaat ttaaacttct cagaataata tatatctcca aattccttta
       61 gagattctaa ttgtaaatct aaaaatgtaa ataggtcatg gtcagttcct ttaaacacat
      121 aatgattatt ttcctttgta aattgtgaag aagtaagttt attttcaata gtagcttcct
      181 tttgtatgtt ggggagcaca aattttttat cttcatcatc atatatatac tttacatcac
      241 aacatatgtt gccatcaatc atatcaaagt agaactcaga tttaaaatct tttgttatat
      301 tattttttat gttatcatct aaatgaactt cttgagatat agattttaat ctaggaacta
      361 gcaatcctaa aacatcctta gaatcctctt tatcaaattt taactcttta gtctttgata
      421 atatttgata tatttttgaa taatcatgag cttttttatg tgataataaa tagatattgc
      481 catcatataa aagtacatca ttttttttag taagtggaac aggtagtgaa tctttagaat
      541 ataagactat tttatcttct atatttttaa aatcaaacct tacaggtaga gtatcattta
      601 atatctttgg atgatattcc tctttagaaa aaataagggt aaaatccttg tacttaatag
      661 catttaaaaa ttgttttaaa gagcttgcat ttatctccat aaattttttt gaatgtgatg
      721 aagaaaagtt gtatgaaaga tttgtcccat attcttctat aatatctact aacttctcat
      781 cttcatcagc aaaataatga gtgttggggt tgtaagtaaa tttcttccca aaatctaatt
      841 catatccatt aagttttgaa tatgcaaatt cagtaatgtt ttttaaaatg tacattttat
      901 taattcctat tttaaattca gcaattgctt tatttgaatc cacatttatc aatacatcta
      961 aattaaccct ttctctttta gtattgtcta acaaagaaag taattgcttg cttaaatcta
     1021 agctggtttt agaatttgta ttagaaagct tatccttatc tgcacctaat atatctatta
     1081 gaataggagg aacatctttt ttatttttta tttgttttaa gctttttatc acaatagcaa
     1141 ctatatgctt acatataaaa atacttttgc cctttgattg acagtcagcg caactacaat
     1201 cattttcaat tatcttaaag tgctttatat ctatagttat tgaattaaaa taaaagctat
     1261 aattatcttc agaattgact ccaccacgaa atgtcaacag attattttgt aggtcatcaa
     1321 tatctatgtc tatataatca actaattgat tagtataata ttttacacct cttgaccatc
     1381 ttgatggttc tgtatttctt cttattagtt tatatatcat atctattttc atattattca
     1441 ccttacctca ctgtatatat tataatctaa atgcattcaa tttacactct aaatttaaga
     1501 ctaaaattaa ggcagatatg aatagctttt atctatcacg atatatgtgt aaataacaat
     1561 ttactattag atttttattg aattatctta taaactatga atgaaaaata attatataaa
     1621 tagattatta ataaatataa aatcttatta ttaaaaatat tcttagaaat tacactgtat
     1681 aaaatataaa gtatgatatt tgaataaaaa gaatgtataa taagtataat aatttgtatt
     1741 tattattggg aaaggaaaat gttaatatgg atagttataa acaacttaaa gctaaaatac
     1801 cctgtaagga aacaggtata gaaataaaga ggactttatg tgatatttgt tcgccaggag
     1861 tacattgtgg aattgatgca tatgttaaag atggtaaagt aataaaaata gaaggtacaa
     1921 aagaacatcc aatgaataaa gggttgttat gtaccaaagg tctttgcaat agagaatata
     1981 tctatagaga agatagaata aaaacacctc taaaaagaat tggaaaaaga ggtgaaggaa
     2041 aatttaaacc aatatcatgg gatgaagctt atagagaaat agcagaaaaa ttaaatagag
     2101 taaaatctga gtatggagca aagcatgtag ctttttttac gggttatgga aagtggtata
     2161 gacctttttt acaaaggttt gcatcttcat ttggttcacc taattatggt acagaatcta
     2221 gttcatgttc aacatcagca cagatggctg gaaatgttgc tactggaaga aactttacaa
     2281 tgcaagatat ggagaatgca aaaacttatt tagggtgggg atttaatcca tactactcta
     2341 aatttttaaa tagtgtgaat ctaataaaat taaaagaaaa aggtctaaaa tttataattg
     2401 tagaccctag aataacaccg acaactcaaa aactagcaga tttacattta agaattaaac
     2461 ctggaactga tggagcttta gctcttggaa tggcgaatct atttatacaa aaggatttaa
     2521 tagatagaga atacatagaa aagcatgtac atggatttaa tgaatataaa gaatatgtaa
     2581 aattatttga tttagatact gtaagtagaa ttacaggagt ttctaaagat aatatagtaa
     2641 aagctgttga gatgttgtca caaggtccat tttgtataaa tgaaagtgca gctccaatag
     2701 ttcaccatca aaatggattt caaaattata gagcaatgat gtctttatcc gctataacag
     2761 gtaattatga tagaataggt ggaaacatac caaaacaaga ttactttgct ggtaagatag
     2821 gtggattcca aacactagaa aaagagtttg taaaagaagt tctacctaaa aatataaatg
     2881 aaagaattgg tggagaaaga tttcctctat ggaatgaaat ggtgactcaa tttcaagcta
     2941 tggatttacc taggcaaata gaagaaaaga caccatatga aataagagct ttgttttgtc
     3001 atgggataaa tgctagaatg tttcctaatt cagggagact ttttaaagcg ttgaatagtg
     3061 tagatttttt tgtgaatata gatttattta tgacagatac atcaaaatat gcagatatac
     3121 ttttacctgc atgtacatca tttgagagag aagaatgtaa gagttatcca ggagggtata
     3181 ttacatatac caaaaaagtt attgataaat tatacgattc aaaatcagat gtagaaatac
     3241 tttcagattt agcaaatgct atgaatatag atgatgattt attaaaagca ggttatgaag
     3301 caagtgtaag acatatgttt aaaaatacat gtgtagatgt agataaactt aaagaaagtg
     3361 atttaccatt aaaatgtaaa gattttaagc catatatagt aggttcatat acaagagaag
     3421 ggtatgatac tagcacttct aggtttgaat taaaatctac aattattgaa aaatataaag
     3481 attttgggct ggatgcacta ccaacatata gaggatttaa tgataatata gatgatgaat
     3541 atactcattt attgacatca ggagcaagaa tttcaaatgc actacattca agacttcatg
     3601 acatcaaact ttcaagaata ttaaaaaatg aagctagttg tgatatttct agcttagatg
     3661 caaaagaatt gggtgttatc agtggagatt atataaaaat aactactaag ataggaagtt
     3721 tggatgtaaa agtcaatgtt actgatagag tcttatataa gaatatacat atgtatcatg
     3781 gatatagaga agctgatata aatagtatta tttgtgataa ttttgaccct tatagtggat
     3841 tccctgcgta tagaagttgt aagtgtaaga ttgaaaaaat gaatagataa atggagagat
     3901 ataatgaaaa gtttatttaa atttaataaa gaattatgta cagcatgtgg agcatgtttg
     3961 cttgcatgta tagaccaaaa tgataataat atagacaatg gtgaactttt tagacatgta
     4021 tttacaatag aggaaggtga aaatatagaa tatttttcat tagcatgtat acattgtaat
     4081 gagccaaaat gtttaggtgt atgtcctaaa aattgtttta aaaaagaaga aggattcgtt
     4141 gtattagaca atcaaaactg tatagggtgt aaactttgtg aaaaagcttg tgaatatgga
     4201 gccttaattt atggaaatga tggtaaggca aataagtgta atggttgtat tgagagatat
     4261 aagtgtggta tggattcacc ttgtgtaatg gtatgtccta caggagcatt aaaatttaat
     4321 aaataaatta aatatagaga atgacttata cttaggtata agatattttt acaaaaagct
     4381 gtattgagtt tgttataatg aaaactcaat atagcttttt tatggatata gttggatact
     4441 ataatgtgat tcttagtaat agttttttgc tattggacaa agactgtaaa acatattaaa
     4501 cttatgttgt tcaaagtcga agtataatat aataactaga aaattggttt atagtgctat
     4561 aaaagcaaat ttagtaaggt ataaaatcag aggcaatcct ttactataat atcaatattt
     4621 gggagttctg ttattgcaga gctattaaac aattataatg gtacaagtga ttcaaccttt
     4681 gaaaatgtat tatataaaaa attaaaataa gattaaaaag gagagaataa attatgaaaa
     4741 aacttaaatt aattattatg tttgccttgg tatcagcact gttagttaca ggatgttcat
     4801 caggaaataa taaaaataaa gaagaacaaa aagttataag agttgcaaca tcaggaacat
     4861 attacccttt tgcttttaaa gataaagata aacttaaagg gtttgaagta gatttttggg
     4921 atgaatttgc taaacgaact aattgtaaag ttgaatgggt attaactgat tttagtggat
     4981 tatttggatt attagaagct gataaagcag atgttgtatc tgctcaactt actgcaacac
     5041 ctgaaagaga gaaaaaatat gctttttcag atgcttataa ttatagtgga acaaacatta
     5101 ttgttagaga agatgatact agtataaaat caatagatga tttagttggc aagaaagtag
     5161 gtgtgggtac tggagcagta gcaaatgaaa tattaaaaga aaaataccca aatgatgaaa
     5221 taaaaatagt aaattattca agtgcgactc ttaaagggaa ttatcaagac ctagaaatgg
     5281 gaagattaga tgcagtagtt gcgcaggatg ttgaggcttt aatagctata aaagaacaaa
     5341 aattaaatct taaaatgata gaacctccaa ttcaatttgg tgcctgttct tttgcgcttc
     5401 aaaaaaatga aaaaggtgaa aaatggacaa aagaaattaa cgaagtaatt gaagagatgt
     5461 ataaagatgg gactttgact aaaatatctg aaaactggct aggaaaagac attactaagg
     5521 agcctattaa tgagtaattt taattttgaa ttttgtctaa gtatatttcc atttttgatt
     5581 aaagcattgg gaataacaat actaatatct attgttgcat tttttttatc aatattatta
     5641 ggtgtaatac ttgccttttt cacacagaca aaatcaaaag ttttacaata cataacaaga
     5701 gtgtatgttt cattctttag aggaacacca cttttaattc aaatattttt cttttatttt
     5761 ggatttccaa tgatattttc ttttatgaaa gattgttcac cttatttagc aatagtaatt
     5821 tgtttaggtg taaattcatc tgcatatatg gctgaaatta tacgagcatc tattgagagt
     5881 gttgataaag gacaacagga agcatgttat gcttttgggt taagtaaata tcagtcaatg
     5941 gttaaaataa ttctacctca agcagctaca tctgcagtac ctcctattgt aagctgtaca
     6001 ctggacataa ttaaaggttc ttctttaggt ttaactatag gaatacaaga tttaatggga
     6061 gtagctcaag tagaggctgc ttcaagtttt aaatattttg agatatatat gataacgata
     6121 ttaatttact gggcagtatc tataatattg agtaatatac aaagcaaagt tgaaaaaaga
     6181 ataagaagag catattagat ttcaatattt aaaaatggga tgatatagat atattattac
     6241 ttgtaaataa atttttaatt ttagaaaaat aaacttagta atctaaatta ttgagaaaat
     6301 atagtatcaa aatggactgt ataatcaagt taaaaaatag tttttctatt gaaaaaataa
     6361 ataaaattct attgtaattt aatatgcgat aaagttttat aaaaaacaac attaagctag
     6421 ggggacttat tttatggctt ttaatatgaa atatttttta gaattatttc caacaataat
     6481 gaaatcgttc aatgtaactg tgattttggc aataagttca ttagtatttt cattaattat
     6541 aggtacaata gtagcgttat catcttatta caaagtgaaa gtattgaatc aaatatgtaa
     6601 agtatatgta tctattttta gaggtacacc tcttatgcca cagttattct ttttatattt
     6661 tggactagca tatatgagtg attttgttaa aaatatggac ccagtatttg caacatctat
     6721 agtacttagt ttaaatatgg gagcttatat gtctgaaacg ataagggcat ctatactttc
     6781 aattgataca ggtcagatag aagcagcata tgcaatagga ttaacaaatt tacaaacaat
     6841 gaaaaggata gtgataccac aggccgtaag agttgcactg ccatcattat tcaataattt
     6901 tatagattta attaaagggt cttctgtagc ttttgttgtt ggagtaaatg atataatggg
     6961 tgctgccaaa tcacaaggag cgttgagttt taagtttttt gaagtgtatg gagcagttat
     7021 ggtaatttac tggtgtataa taactccttt aggttggggg caaaaaaaat tagaaaagaa
     7081 aataagcaat tattaagtta ttaagaaaat acataaaagt atttttatat aaaaaatggt
     7141 gtgaggagaa gagatatgat aaaaagaaat attataaaat gtttagtggc agttttatcg
     7201 ttaacgtttg taattactgg ttgttcaaaa aaagaggaag taaagaatga aaaagttata
     7261 actgtagctt catctaattc agctgaccca tattcttatg ttgataatgg tgagcataag
     7321 ggttttgaag ttgacatgtg gaaagagatt ggtaaaagaa gtggatataa gataaaaatg
     7381 gaaccaacag gttttagttc tatatttggt atgttagatt ctggtaaagt tgatgtggca
     7441 gccaacttct ttggaatgtc taaagaaaga ttagaaaaat acgatgcttc tataccttat
     7501 ggttctgatt ctgtgtgtat agctgtaaaa gatggaaata acgaaattaa taagcttgaa
     7561 gatttaaagg gaaaagttgt agcagtttca gaaggttctc aaggtcaaca ggttgcaact
     7621 agtgttaata aagataattt gtttcaagaa aaggtttacg gtgatggaac tagtggtctt
     7681 caagatttag atttgggaag agttagtgca tggattgatg cagagcttac tattgtggga
     7741 gacgctaaaa aagctaatat gaaagtaaga gtattagata aaaaattatc agctactaat
     7801 atagcatact tctttaagaa aaacgatgat aaatctaaaa cgataaaaga agatgtaaac
     7861 aaagcaatag aagaaatgct tgcagatgga acagtgaaaa aaatatctga aaaatggttt
     7921 ggaatggatg taagtgctga tatacaaaaa taaatacttt aaaattaaaa tgtaaaactg
     7981 tgataactat agataagtat actaaattta tctatagtta tctgtgtatt acaatgtatt
     8041 tagattagtc tttataaatc aaaatgagtt atggaggtat tgcaaatgat tgaaactaaa
     8101 ataaaatcca ttgcactaaa aaatagagaa aaaattataa aaataagacg acagatacac
     8161 agtaatccag agttagcatt taaagaatat aaaacatcta aacttataaa agaagagcta
     8221 aataaactta atatagaata cataaatatt gctggtacag gagtattagc tactataaaa
     8281 ggaaaaaata atggtggaaa aactatatta ctcagagcgg atatggatgc tcttccaatc
     8341 aaagaggaaa atgatttaga atttaaatca ataaatgata atatgcatgc ttgtgggcat
     8401 gatgcacatg tttcatggtt gttaggaaca gcaatgattt taaatgacat aaaagaagaa
     8461 ttgaatggaa atgtaaaatt actatttcaa cctggcgaag aaaaaggtgg ttctgatatt
     8521 gtaattaaag aaaatgtact tgaaggtgtg gatgcattag cgactggtca ttgttggcca
     8581 actattgagt caggaaaaat tggaatcgct agaaattgtg ctatggcagc gactaatact
     8641 tttgaaataa caataattgg caagggtggg catggagcag agcctcataa ttgtatagac
     8701 ccaattgcag ttggaaatgc agtatattca tccattcaac agatagtttc aagaaaaatt
     8761 gacccagttg tacctgtagt agtgtctgtt tgttctttta attctggtgt ttctaaaaat
     8821 ataataccag atgtatgtac acttcaaggg actattagag cgataagcaa ggaacaagta
     8881 attgaagtaa gtaaaattct agaatatata gtaaaaaatg tatgtaaagc acatggagca
     8941 gattgcaaat ttgaaaagag tacaggagga gatgctgtta taaatgacaa aaacatgata
     9001 gagataggaa gagaaagtgc ttgcaaaata cttggagctg aaaatgttga aataattgat
     9061 tttcctgcaa tgacaggtga ggattttgct atttatatga aagaaaaacc tggtttattt
     9121 atgtatatag gtgtaggaga taaagaaaaa aatataaatt acagattgca tagtaacaag
     9181 tttaatatag atgaaaaatg tcttagtata gcaccttcat tattttcaca acttacagta
     9241 gattatttat gtcaactata gagtacattc ataaaaatat atttagccta tttttaaatt
     9301 taatgctagt atattaattt tgatgtagaa catattattt attatattta tttaaaaagg
     9361 tggataacaa tgataaaagt agaaaattta cataaaagtt ttaaagggac agaagttctt
     9421 aaaggtataa atttagagat aagtcaaagt gaagtagtag ctatacttgg atcatctgga
     9481 acaggtaaat ctacgttact tagatgttta aattatttag ttgagcctac aaaaggaaag
     9541 ataatgattg gtgatgtaaa agtagatgta gaaaaagcaa caaaaaaaga tataaccaat
     9601 ttaagaaaac atacatctat ggtgtttcaa aattataact tatttaaaaa taaaacagca
     9661 ttagaaaatg taatggaggc attgatagta gttcataaaa agagtaagaa agaagctaga
     9721 gaaatatctt taaaattaat agataaagta ggtatgttgg ataggaaaga tttttatcca
     9781 tcaaagttgt ctgggggaca gcaacaaagg ataggtatag cgagagcgat ggcagtaaat
     9841 ccaaatgtaa tattatttga tgagcctaca tctgcacttg acccagaact tgttggagaa
     9901 gttttaagcg ttataaaatc tcttgcaaaa gaaggtataa cgatgcttat tgtaactcac
     9961 gaaataaatt ttgcaaaaga agttgctgat aaaattatat ttatggataa tggtgtaata
    10021 gctgaagaag gaactcctga tgaaatattt aataatccta aaaatgaaag aacagcaaaa
    10081 tttttaaata tcattaggaa agaagaagta tttagttaaa agtgtataaa ttaagcatta
    10141 gaagatatgt aagatacccc cattaaattt ataagttag
//
LOCUS       DAGSWI010000143        10049 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.144, whole genome shotgun sequence.
ACCESSION   DAGSWI010000143 DAGSWI010000000
VERSION     DAGSWI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.144"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            288..1763
                     /locus_tag="MJF80_003241"
     CDS             288..1763
                     /locus_tag="MJF80_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBZ0281879.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1850..2263
                     /gene="speD"
                     /locus_tag="MJF80_003242"
     CDS             1850..2263
                     /gene="speD"
                     /locus_tag="MJF80_003242"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBZ0281880.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2293..3144
                     /gene="speE"
                     /locus_tag="MJF80_003243"
     CDS             2293..3144
                     /gene="speE"
                     /locus_tag="MJF80_003243"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBZ0281881.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3134..4012
                     /gene="speB"
                     /locus_tag="MJF80_003244"
     CDS             3134..4012
                     /gene="speB"
                     /locus_tag="MJF80_003244"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBZ0281882.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4192..4392)
                     /locus_tag="MJF80_003245"
     CDS             complement(4192..4392)
                     /locus_tag="MJF80_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBZ0281883.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            4859..6349
                     /locus_tag="MJF80_003246"
     CDS             4859..6349
                     /locus_tag="MJF80_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBZ0281884.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            6629..8125
                     /locus_tag="MJF80_003247"
     CDS             6629..8125
                     /locus_tag="MJF80_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBZ0281885.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            8259..9185
                     /locus_tag="MJF80_003248"
     CDS             8259..9185
                     /locus_tag="MJF80_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBZ0281886.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            9303..9494
                     /locus_tag="MJF80_003249"
     CDS             9303..9494
                     /locus_tag="MJF80_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281887.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            9717..>10049
                     /locus_tag="MJF80_003250"
     CDS             9717..>10049
                     /locus_tag="MJF80_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281888.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRK"
BASE COUNT         4030 a         1048 c         1806 g         3165 t
ORIGIN      
        1 actaaaactc aaaaaagtaa aaaaaccaaa ataataaaaa ataagaaagt ttataaatac
       61 taagttttaa aaaaattaat ataaacaaca aaaaattaaa aagtttataa atattgatat
      121 agctaatgta tatgtttttg agtaacttca aaaaaacact tgtaaaaatg ctgggatgtt
      181 aatataataa atatcatgag tttaataaaa gtaaaaataa aaaatagaaa taataaataa
      241 taaatattaa aaatatttga atatagagag gagatgaatt tgaaatcatg gatagcgagt
      301 ttgcaaatgc agttgaatta agctatgagt gtgctccatt attagagtcg ttaaaggaat
      361 actcagagaa agatatagct tgttttgatg tgcctggaca tgtaaaaaat agaggtgtag
      421 ctatattaaa taaatatcta ggtgaaagtc tcatgaaaat ggatataaat tcatcaccaa
      481 ctatggataa tgtatctgcg ccaaatggga ttataaaaaa tgcacaagat ttgctagcac
      541 aagcatatat ggctgatgag gcattcttta taacgaatgg cacaactcaa gcaatacatg
      601 caatgatttt gagtgttata aaaccaggtg aaaaagtatt acttccaaga aatatacata
      661 aatcagtaat aaatgcgtta atactttgtg gaggaatacc aatatttata caaccagaat
      721 ttgatgaaaa actaggtata agtttaaata ttactcttga aaaagttaga acagagatag
      781 aaaaagattg taatataaaa gcattatttt tccttaatcc aacttactat ggagtttgtg
      841 cagaccttga gagtattata gaactatgcc ataaaaataa tgttttagtg ttagttgatg
      901 aagctcatgg tgcacatttt ccatttcatt tagatttacc tccttctgca ataagtttgg
      961 gagctgatat ggtagctgta agtatacata aaactggagg agcattgaca caatcttcag
     1021 cactcttatt gaatagagat aatgtaagtt ttgaaaaggt tcttcaatca atcaatatgc
     1081 tacaatcaac atcagcatct tatttgctta tggcaagtat agatggtgct agagttaatc
     1141 tagttgagaa tggtgaaaag caactgtcaa aagcattaaa tctatctagg tatgcaaaat
     1201 ctaaattaaa caaaattgat ggaataaaag ttttatctac agaaatattg aaacaaaaag
     1261 gtgttaaatt tatagatgaa acaaaattgt gtataaatgt aaaagaatta aatttgactg
     1321 gatttgaagt atacgattta ttatataaaa atttttcaat tcaagtagaa ttgggagatt
     1381 catataatat actggcactt gtgtccattg gaacaaataa atcagatata gatagattag
     1441 taaaagcact ttctataatt gcaaagattt atagaaaaga atcaacatta aatgaattta
     1501 atatggttca aataaatcca attattaaac ttaatccaag agaagctttt tatgcaccaa
     1561 aggaaagtgt agaaatcaat tcatgtatag atagaatttg tggagaatca ataatggcat
     1621 atccaccagg tataccgatt attgctccag gagaattgat aacagaagaa attatggagt
     1681 atattatttt cttaaaaaat agcaatgcat atcttacaga tgtacaagat aaaaacttag
     1741 atagaatatt agtaataaaa taaattactt tattttgcaa gggcaagtat ttgttaagta
     1801 gagtttatca aatagaactt attaaaagta taaaggagtt ggggttgaaa tgaaatttga
     1861 aacactagga agacatatac tagtagaata ctataattgt gatgaaaacg tacttaaaga
     1921 cccacgtctt atagaaaatt ttatgaatga ttcagcatta aatgcaaaag cgacaattgt
     1981 ggattctgta ttccaccatt ttaatccttg gggggtttca ggagctgtta taattgctga
     2041 atctcatttg acaatacata catggcctga atatggctat gcagcagcag atttttttac
     2101 ttgtggtgat atagacccat ggaaaagttt tgaattatta gagcaacttt taaagtctga
     2161 aagaagcgag tctacagaaa ttccaagagg cttaactaca aaaattaaaa aatattcaaa
     2221 aaaagattta ggtaagatta ctcataaacc agaagcagta taaaacatac aagagaaagt
     2281 gaggattatt atatggaact ttggtataca gaagaatgga cagaaaatgt aagattttct
     2341 ataaaagtaa ataaacatct atttgaagga aagtctcaat ttcaaaggat agatgtgttt
     2401 gatagtgatg aatttggaaa atttctaaca atagatggat taatgatggt aacatataaa
     2461 gatgaattta tataccatga gatgataact catgttccta tggctacaaa tttaaacata
     2521 aagaaagtat tagtcatagg tggaggtgat ggaggtacag taagagaatt aagccgttat
     2581 cctcaaatag aaaagattga catggttgaa atagataaaa tggttgtaga tgtatctaag
     2641 gagtatatgg atatatgctc atgtaaatta gatgataaaa gagtgagttt atattttgaa
     2701 gatggtgtta attttgttaa gtgtgcacat gataaatcat atgacctgat aatagtagat
     2761 tcaactgacc caataggacc aggtgaaggg cttttctcaa ctgattttta taaggattgt
     2821 tatagaatac tgactgatga tggaatttta gtaaatcaaa gtgaaagtcc gtattttgac
     2881 tttaatgcta aagagatgaa aagagctaat aaaaaattaa aacagatatt tccaatatca
     2941 gaagtttatc aagcacatat accaacatat ccatctggtc actggttgtt tggatttgct
     3001 tcaaaaaaac ttaatccagt aaaaaatcaa gatagaaatg gatgggagaa attaagttta
     3061 aaaactaaat attataatag tgatatacat ttaggttcgt ttatgttacc tcaatacgtg
     3121 aaggagatgc tagatgaaga ataatttcta tcatatgaac acttttatga gtatggacaa
     3181 aaattatgaa gaatctaatc ttatagtatt tggtgttgga tttgatggaa ctacttctaa
     3241 cagacctggg gctagatttg caagtagctc tatgagaaaa gaattttacg gtcttgagac
     3301 atatagtcct tttttagatt tggatttaga ggattataat atatgtgatt atggagattt
     3361 agaaattagt gttggaagta cagaacaagt cttaaaagaa atctatcaag agacatataa
     3421 gattgttaga gattcaaagg taccctttat gattggagga gagcatttag ttacattacc
     3481 agcctttaaa gcagtacatg aaaagtacaa tgatatatat gtaattcatt ttgatgccca
     3541 tactgatttg agggaagaat ataataatag taaaaattct catgcaacag taattaaaag
     3601 aatatgggat attgtaggtg ataataaaat atttcaattt ggtataaggt ctgggacaaa
     3661 agaagaattt aaatttgcta cagaagaaaa acacacatac atggaaatag gaggaataga
     3721 tacgtttgaa aatatagtta atatgctaaa tggaaagaat atttatctaa ctatagattt
     3781 agatgtattg gatgcatctg tgtttccagg aacaggtaca ccagaacctg gtggtgtaaa
     3841 ttatagagag tttcaagaga tttttaagat tataaaaaac tctaatataa atatagttgg
     3901 ttgtgacatt gtagagttaa gccctgatta cgatacaaca ggtgtatcga cagttatagc
     3961 ttgtaaaatc ttaagagagt tatgcttaat aatatctgat aaaattaaat agagtatttt
     4021 atataaatag tatatattat aatataagaa gttattttaa aaaagttagt ttaagtttga
     4081 cgcttttcta aagtaacttc ttatttttgt aattaaaaat aaatataata aacaacaaaa
     4141 aagagccctc gatagctcct tttctaaaat atatatattt aaattaaata attatattct
     4201 atttacgttt tcagcttgag gtcctctatt accttgagtt atatcaaagc taaccttttc
     4261 gccttcttct aaagttttgt atccatcatt ttgtatagct gagaaatgta cgaaaacatc
     4321 atcttctccc tctacagata tgaacccaaa tcctttttca ttgttaaacc attttactat
     4381 tccgtttttc attataagaa aacctccgca ttattaaaaa atacaaacta aaaaaataag
     4441 tctgttctat taatataaca taaattttaa ataaaagcaa ataaaatttt taaaaaaatt
     4501 aattactttt agaatttaat aaaaattgtt aaattataca gtgagaagta ttgtaaaatt
     4561 tgacgaaaat gtaaatgtag ataataaatt taatatcaaa aaatgtaaca aaaaggtatg
     4621 taagtaaaaa gtaaaattaa aatatataat aaaaaatatt atatattaga ttgaaaagta
     4681 aaaataaaaa tagtgaaatt tcagaaatat caataatatt atataataaa acaaaaaaac
     4741 actatatata ttcgataaaa taaaatttaa acatataata ttttatgtat atattaataa
     4801 aaggacatat tgtgttataa tggtatcgta taaaatcatt tacgggggtg gtattaaaat
     4861 gtttcaattt gaaacacaaa tacacaaatt aagacatgat gttttaactg caatagctag
     4921 acttggaaaa gaagataacc ttaccagaga agcaatggat aatattcagt atgaaataat
     4981 aaaaggtgag aaacctacat acagatgttg tgtttataaa gaaagagcta taatatctga
     5041 gagagtaaat gtaagtatgg ggttacaacc aggtcaagat aagatagatt tagatacaat
     5101 ggatatagaa gaagatgaac aaattatata tattttggaa gctgcatgtg acagttgtcc
     5161 aataaatgaa tttacagtaa cagatatttg tagaggatgt ctagcacata gatgtaaaga
     5221 agcttgtaaa tttggagcta taacacatgt cggaggaatg gcctatataa atcatgaatt
     5281 atgtaaggct tgtggaatgt gtaaaaaagc ttgtcaatat gatgccatct cagaggttgt
     5341 tagaccatgt aagagtgttt gtccaactaa tgccttagga tttgatagag aaaatatgaa
     5401 agctatgata catgaggata aatgtttaaa ctgtggagca tgtatgtcag catgtccatt
     5461 tggagcaata tctgataaga gtttgatagc tcctgtagct agaaagttag ttcaaaaaga
     5521 gaaaatgtat gcggtggtag ctccagctat aacaggtcaa gttgaagcaa atgttacata
     5581 tggtcaaata aaaaatgcta taaaatcact aggatttgtt gatgtattag aagcagcttg
     5641 tggagcagat gcagttacag ttcatgaaag cttagaattt gctgaaagaa tggaaaaggg
     5701 agaagctttt atgacaaatt cttgttgtcc aggatttgtt ggttatatag aaaaaacata
     5761 cccaaatcat gtagggaaaa tatcttctac agtatctcct atgatagcta gtggtagatt
     5821 tataaaaagt catgaaccag atgctaaagt tgtattcata ggaccatgta cagccaaaaa
     5881 gagtgaggca gctaagaaag aattaaaagg agctgttgat tatgtactta cttttgaaga
     5941 aataatggca ttatttgagg catttgatat agacttggct aagtgttcaa atgaagatat
     6001 agatgatgca tcaatttttg gaagaggttt tggagctgct ggtggtctta caaaagctat
     6061 agaaaattat cttgctgaaa aaggtattgg tgttaatttt gaacctgtaa agatttctgg
     6121 ttcaagagaa atcaagaaaa ctatgactat ggctaattta ggaaagctag aaggtaattt
     6181 tatagaagga atgatgtgtg aaggtggttg tataaatggt tctggtaaat tcttatcagg
     6241 tgcaaaggtt aaagccactt ttgatagaaa aaattctcaa tcaactaaga aaagtgtctt
     6301 agcaaataca aagataaaag attttagtaa tataaattta gaaaaataaa gataatacaa
     6361 agcaacaaaa atacatattg ttaatagagt tgttctaagt tgaggtttaa cttttggaca
     6421 actctatttt tatttatata tttaattatt gaacaactaa aataaaaatt atatacactt
     6481 aaaataaaaa ttacatatat aatattttgt atataactta acaaagtata tgcctatgtt
     6541 ataataattt atagaaataa ataaaaaata caatatacat aatagtagaa atatagcaaa
     6601 aaggtgtgat ttatagggag gattacttat gtttgaattt gaaacgcaac taaaaaggtt
     6661 aaaacatgaa gttttaacag cagtagcaag actatctatt gaagaaaatt taacaaaaga
     6721 aaatatagaa aaaattccat atgaaataat taaaggagaa aaggcgagat atagatgttg
     6781 tgtttataaa gagagagaag tagttcttga aagggcgcaa cttgcattaa gtttaactcc
     6841 aaatagtaaa tatggagata taaatccaga ttcagttagt ttggacacag atgagcaaat
     6901 tatatatata gttgaagcag catgtgatag atgtccaata aatgaattta cagttacaga
     6961 gctttgtaga gggtgtttgg ctcatagatg taaggaagca tgtaaatttg gggcaatttc
     7021 ttatataaat gggagagcat atataaatca tgatttatgt aaggcttgtg gaatgtgcaa
     7081 aagttcatgt caatatgatg ctatatcaga agttgtaaga ccttgtaaaa gcgtttgtcc
     7141 aacaggagct ttggatttta atagaaacac tatgaaagca atgatacatg aagataattg
     7201 tataaattgt ggagcatgta tgtcagcatg tccttttgga gcaatatctg ataagagttt
     7261 gatagcacct gttgcaaaaa aacttgcaaa gaaagaaaat atgtatgcaa tagtagctcc
     7321 agctataaca ggtcaaatag atagtaatat tacatatggt cagttaaaaa atgctataaa
     7381 atcactggga tttgtagata tgatagaagc ttcttgtgga gcagatgcag ttacagttca
     7441 tgaaagcaat gaacttattg aaagaataaa atcaggagat aattacatga caaattcatg
     7501 ttgtccggga ttttttaagt atatagagaa gatgttccca gatgaaaaag atagaatatc
     7561 atctacagtg tctcctatgg tagcaacagg aagattagta aaaaaaatag attctgatgc
     7621 taaagttgta tttataggtc catgtacagc taaaaagagt gaggcaacag agaaaagttt
     7681 aaaagattca gtagattatg tacttacttt tgaagaacta atggcattat ttgaagcatt
     7741 taatatagat ttatcaaaat gttcaaatga agatataaat gatggttcga tttttggaag
     7801 gggatttgga gcatctggtg gacttgccaa atcagtacag aactatattg ctgaaaaggg
     7861 gcttagtttt gattttgaac ctataaaagt atctggtcca agacaaatta aaaagacaat
     7921 gacaatggct aaattgggaa gattagaagg taattttata gaaggaatga tgtgtgaaga
     7981 aggttgtatt aatggagccg gtaagttttt atcaggtgta aaagccaaaa atacttttga
     8041 tagaaaaaat ggacaatcta ctaaaaagag tgttttatct aatgataaga ttaaagaatt
     8101 tgaagatgta aatttagaaa gataattgtt ttggtacagt cattttttta ttatgccaca
     8161 aatatgccta aaatttgccc tttttatatg tgtattttac agagttaagg ttgctatact
     8221 ataaatatgt aataaataaa atttatagga gcttttggat gaggaaaata gtattaaaag
     8281 atatcctgtt tttagttact acattatcta taatagtaat attattgctt gtaacaaata
     8341 taactactaa tagtaagata aaatcaacaa gtgaatctta tttaaaaagt gacaaaaata
     8401 gtgtgaggca aaatggtatc cagaggagca caaagagtag cacaaaaaat actgcattta
     8461 agactactat actaaacact ggaaagtctg attgcatatt ggttgagata ggtaacaaag
     8521 tcataatgat tgatacagga gaagataaaa atggaaaaca aatagtggat aggttaaaag
     8581 aaaaaggaat aaatacactt gactatttga ttttaactca tttagataag gaccatatag
     8641 gtggagtaga tagtgtgtta tctagtgtta aagtaaagaa tattatacaa gcaaattata
     8701 agaaagatag taagcaatat gatgaatata ttgactcact taaaaaagct gatatagaac
     8761 cagtgctact aaaagataat atgaatattg taatcaatgg tgcagaaata aacatacatc
     8821 cggcttcaaa aagcaagtac gagagttcaa atgattattc aataatcact aatataagct
     8881 atggagcaca taagttttta tttgcaggag atgcagagga agaaagattg tcagagttta
     8941 taaatggaaa tactttaaaa tatgattttg ttaaaatgcc tcatcatgga agatatgaca
     9001 aattaactga aacattttta gagtctattt cacctcaata tgctgttatt acatgttcag
     9061 agaaaaaaga accagaggaa ggtgttttaa aaatattgga aagattgaat ataaaaacat
     9121 ttttgacttc taatggagag gtagttatca atagtgatgg taagacattg agtgtgaatc
     9181 aataattttt ttaaaaatat ctaaaaaaat ttaaaaaaat cattagcaaa tattacccct
     9241 taaacatagt atattttagg agctgaaact ccacatacaa aatgactagg aggtaacata
     9301 ttatgttgga tagatttttt ggtgaaggcg gatttggtgg atgtgcttgg tggataataa
     9361 tattattctt cttattctta gcattccaag attgttgggc tgaaatggac ttatgtgcat
     9421 ggataccttt cttaatatta ttattaatac tttgtgaatg tggtgacgtt tttgatggag
     9481 gatgtggatg ctaaaaaaat caatttagat gatttaataa agcttatggt gggtataaat
     9541 aaaataccaa accaatcaca atctagtgtt tgaaggtagt cggaaatccc ccgctacctt
     9601 ctttttttta ttgttaaaat ttatttttga attatttcat ttacatataa taatttttac
     9661 gataaagtct gatataatta aaaagaaaat aaaatttatt tataggagat gtaaaaatgg
     9721 ctatagtgga taagattttc aatgagataa aacttatggt tgcaagactt ttattgggaa
     9781 aaaaatacag tgaatatgaa gaaaaagatt tagtgtataa tacagaagaa gaagttatac
     9841 ttataacttt aaaaagatta gtttttcagg gaaatgtaaa cgaagcagaa gaaattttat
     9901 ttgataaagc taagagtgta aatagtgaaa atatgcagta tatagcaata gaattttata
     9961 ctatgcttat ggaaaagact gatgaagaat tagaagctat gaatttttct aaacaagaag
    10021 tttatcaagg tatagaagat attagaaaa
//
LOCUS       DAGSWI010000144         9876 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.145, whole genome shotgun sequence.
ACCESSION   DAGSWI010000144 DAGSWI010000000
VERSION     DAGSWI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.145"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            235..1068
                     /locus_tag="MJF80_003251"
     CDS             235..1068
                     /locus_tag="MJF80_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBZ0281889.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1161..2093
                     /locus_tag="MJF80_003252"
     CDS             1161..2093
                     /locus_tag="MJF80_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBZ0281890.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2145..2627
                     /locus_tag="MJF80_003253"
     CDS             2145..2627
                     /locus_tag="MJF80_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBZ0281891.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQESMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2639..3508
                     /locus_tag="MJF80_003254"
     CDS             2639..3508
                     /locus_tag="MJF80_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBZ0281892.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIRAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3551..4306
                     /locus_tag="MJF80_003255"
     CDS             3551..4306
                     /locus_tag="MJF80_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBZ0281893.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNELLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVNKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4348..5169
                     /locus_tag="MJF80_003256"
     CDS             4348..5169
                     /locus_tag="MJF80_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HBZ0281894.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5174..5935
                     /locus_tag="MJF80_003257"
     CDS             5174..5935
                     /locus_tag="MJF80_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBZ0281895.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKINPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIFVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6096..6353
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6466..6705
                     /locus_tag="MJF80_003258"
     CDS             6466..6705
                     /locus_tag="MJF80_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBZ0281896.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
     gene            7009..8004
                     /gene="ilvC"
                     /locus_tag="MJF80_003259"
     CDS             7009..8004
                     /gene="ilvC"
                     /locus_tag="MJF80_003259"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBZ0281897.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDHT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            8057..9745
                     /gene="ilvB"
                     /locus_tag="MJF80_003260"
     CDS             8057..9745
                     /gene="ilvB"
                     /locus_tag="MJF80_003260"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBZ0281898.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
BASE COUNT         4217 a          949 c         1764 g         2946 t
ORIGIN      
        1 atgacatttt aactagaatt aattgtaaaa aacactatat aatactcaat tttcaacatt
       61 ctatacaatt atacagaaat tatacagcat aataatcacc gaaataacaa attttaaaaa
      121 ttaagcaaaa ctttttgtcg aagttcaacg tctatatata tgtagtgtaa atttttggga
      181 taaaatacta caaaaatata aatgtaaaaa tatagtaata ttggaggtcg aacgatgaga
      241 aataaaaaga aaaaaataaa tagaaagaag ttgtatttgc ttttaagtgc tattgtagtg
      301 tgtctggctt ttatagtagt tggtgttcgc gtcagctctt taaatatgaa ggaaaatgaa
      361 attagtagaa atgcaaaact gtcaagttct actattacag atatagtatc taatacatca
      421 atagaagtta gtaaagggcc aagtaaaagt tctggaaaaa tagcatacat aactatagat
      481 gatggtccat ctaaatttac tgacgagatg ataaaaactt taaataaata caatgttaaa
      541 gcaacttttt ttatgataga tgggaatatg aaggaatatc cacagcaagt taaaaatata
      601 attaaaaatg ggaacacagc gggttttcat agtgtatcac atgatattca caagctttat
      661 gttactagta catcagcaaa agaggaattt gatacaaatg atcaaacttt ttataagata
      721 actggtaaac attcaaaagt aataagaata ccttatggaa gcaaaccata tacaccacaa
      781 gcctcttatc aggctttagt tgatgctgga tataaaattt gggactggga cttagataca
      841 gaagactgga gatctaattc ttctcaaatt gtacaaaatg taaaaaatca tattaagaat
      901 agaaaaggtg aagataaaga ccaacttgta gttttaatgc atgagaaaaa acagagtgca
      961 gaagctctag attcagtttt aaaattctta tcagatgaag gttatgaatt tgcgccagta
     1021 gatcaaaacc aaataccaaa aaattattgg ttacgtaatt tagagtagtt aaaatataag
     1081 aatattgatt gatattttta gaaattgaga ataaaaatga gtgaggtgta ggtatattga
     1141 ataaaaaaat ttatatagga atggtcggaa tattgtcttt gatgatggtt ggttgtaata
     1201 aaagtttagc aaaagtaaat gatgtggaaa tcacaaaaga acagtacaaa aaaacaaaag
     1261 cagttttatc tgctactaat aattatataa atgggcagtc tttagatgaa ttagaaaaaa
     1321 ctttggataa aaaaggtaga aataaattag aaaatgttat aatatcgttt atggtggata
     1381 atgaactttt atatcaagag gctaaggata aaggactaac tccaagtaaa agtgaagtag
     1441 actcaaaata tcaagaacta gaagataaga tgaatttgaa tacaagctat aaggaaaaaa
     1501 tggacaaagc aggtgttgat aaagattatt taaaacaaga aatatctaga gatttagcaa
     1561 tagataaaaa taaaaaggcc tttgaagata gaataaacat aagtgataat gatatggaag
     1621 cctactatac aagtcataaa aaagatttta atgtagaaga agtaagtgct tctcagatac
     1681 ttatatcgac tttagataaa aataaaaaag aagtaagcaa agataaaaaa gaagctttga
     1741 agaagaaggc agataatata ttgactaaaa tcaaaaatgg agaaagtttt gaaagtttgg
     1801 ctaaaaaata ttcggatgat aaagcaactg gaaaaaatgg aggacagcta ggatatttta
     1861 caaaggatga taaaaatgct gagtttacaa aggaagtgtt caaattaaag aaaaatgaag
     1921 tttcaaatgt gtttgaaact agttatggat atcatatagt caaagttact gataaaagag
     1981 aaagacaaaa aagttttaat gagtgtcaga gtttaataag agaatctatt ttaaatgaaa
     2041 agtatattga gcatataaaa aaactgaatg aagatgctaa aatagacaga tagaatttct
     2101 aacagctcaa tcctagcctg taatcggagg gagattttac aggaatgttt aagagtttaa
     2161 agagaacgaa agttgagaaa tacattatcg ataataaaga ctctttttat agaatagcat
     2221 atagttatac gaagaatgaa gaagatgcct tagatgtggt tcaagaatcc atgtataagg
     2281 cactatactc agtggaaaat attaaagaag taaattatat aaagacatgg ttttataaaa
     2341 ttttagttag aacgagtata gattttatta gaaaaaaccg taaatataat aatatgacag
     2401 atattgacct tatagatgag actggagagt atgataaata tactgatttg gatttaagaa
     2461 gagctttgga agaattacct attgaataca aatctattat aatattaaga ttttttgaag
     2521 atttaaagat tgaagaagta gcaataatat tggatgaaaa tgtgaatact gttaaaacta
     2581 ggttatatac tgctttaaaa aaactaaaat taaaaattga agagtaggga taaaaagaat
     2641 gacaagcaaa gaaaggttaa ataagttaaa agatgagtat cataaaattc caatacccaa
     2701 gaaattagat actataataa atcatgaaaa aatagaaaac atatatcgag agaaaaataa
     2761 aaaagtaaat agattaagat ttaaagttgc aatagccttt gcatgtatat ttacagtatt
     2821 agtaaatata agtcctgtat ttgcagacaa tttttctaag atacctgtaa ttagagcaat
     2881 agtagaagta ataactataa aaaattatag tttaaaaagt gaaaattatg aagctgagat
     2941 tgatattcct aaaatacgag gtttaaaaga taaaaactta gaacaaagat taaacagtag
     3001 ttttatggaa gatggaaaaa gactttatca tcaattccaa gagagaatgg agaagataca
     3061 atcatcaaag aataaaggat ataaatcttt aagtttgagt tatagtgtaa aaaataattc
     3121 taaaaaattt ctatcaatag agatgacaaa aaatgagata gaggcttcat catatgtgag
     3181 taaggtacat tatactattg ataaaaaaag acaaatagtt ttgactttgc caatgctttt
     3241 taaagatgat aagtatataa aagttataag tgataatata aaagaacaaa tgagagaaca
     3301 aatgaaaaag gatagtacaa aatcctattt tatagatcaa aaaaaagact tgcctgttga
     3361 agattttaaa acaattaata agtatcagga tttttatttt aataaaaatg aagaccttgt
     3421 aatttgtttt gatgaatatg aagtagcacc tggttatatg ggcgcagttg agtttgtaat
     3481 accatataaa gtaataaaag atttgtaaat tgaatagtag tttatatata aatcttttaa
     3541 ggaggagaat atgactacat ataaaaaact taaaaaatct atatttaaaa ttgctatatt
     3601 ttcaatatgc ttattagttt cattagtaga tacaaaccct atatttgcag gtagttcctt
     3661 taatgaaact tcaaatagtg atacgataaa ggtgttaaat gctaagagta tcaactttaa
     3721 acaagacaat tatgaagtga atgtgagtat tcctaaaata aaaggtttaa aagataaaac
     3781 tttagagaat aaattaaaca atgagctttt gcaagatggg aaaaaaattt acagtgaatt
     3841 tcaaaataaa atgaaaaagg aagataataa gggtcataga tacctaaatg taatccataa
     3901 tgtcaaaaat aattctaagg actttttatc aatagaaatg acaaaagaag agatagaagc
     3961 ttcctcatct ataactaaga agcattacac tgttaataaa aatagaggaa tactattaaa
     4021 tttaccaata ttttttaaag atagtagata tattagagct ataagtgata atataaaaga
     4081 gcagatgaga aatcaaatta agaaagatga tacaaaatct tattttatag accaagataa
     4141 agactcgcca gtgaaagatt ttgaatctat aaataagtat caagattttt attttaataa
     4201 aaatggaaat cttgtgatta gttttgatga atatgaagta gctcctggtt atatgggagc
     4261 agtagaattt gtgataccaa ataaagtaat aaaaagacta agatagatat atagaagatt
     4321 tttagataga ctggtaagga gaattatatg aatatatatg aacaagcaat aagattaata
     4381 gaaaataatg aggatttcgc atttgctaca ataacgtctc attcaggttc aacaccacgt
     4441 gaaactggag caatgatgat tgtgaaaaat gattctacta tatttggctc agtaggagga
     4501 ggtagtgtag aagcagaatg tataaaacat gcaattaatg ttataaaaaa cagagaatca
     4561 atgttgtata aatttactct aaacaaatct gatgttgcaa aactaggaat gatatgtgga
     4621 ggtactggag aaatacaaat agattttata gatagtaaat taaaaagtaa tatagaaaaa
     4681 tttaataaga gactaaagga aaatacaagt aaagcatata tatttggtgc aggtcatatt
     4741 tcaagagatg ttgctgtgat actatctctt ttagaattta gaacagtagt aatagatgat
     4801 agagaagaat ttgctaatca tgaaaggttt ccagaatcag aagtagtagt tttagattct
     4861 tttgaaaaca taccagattt tccaactgac gaaaatagtt atataattat tttaacaaga
     4921 ggtcatctat atgattcaag tgctttagag tgggctctta aaagagaggc tggttatata
     4981 ggtatgattg gaagtagaac aaaaattgga ttgacttacg aaaaactcat gaaaaaaggt
     5041 tttaagaagg aagaattatc aaaagtacat gcaccaatag gcataaaatt gaatgctcaa
     5101 acaccagctg aaatagctgt atgtatagct gctgaactta taaattgtag agctaataag
     5161 gaaaaataaa ctaatgaaca cagggagtaa agatattgat actttaaagg ttaattttag
     5221 agggattgaa ataaaagcag ttttagtata taaaaataga aaaaatattt ctataaaaat
     5281 aaatccattt ggcaaaataa ctgtaatgag tcctcctaaa atatccaaaa aaataataaa
     5341 agatataatt attgaaaaag gagactggat acttaaaaag tcagaaaaat gtaaaggaag
     5401 agaggaagta tataaacaaa gaatgtttat aactggcgaa aaatttcttt atttaggtga
     5461 agagtaccat ctagttataa aagaactgtt gagagattct gtcaaaaaaa gtaactgtag
     5521 aataactatt aacgaatatc aaatatttgt tcagacaaat gatacaagta cagattttat
     5581 taaaaaatct ttaaaatctt ggtataagat ggaaagtgaa agaatagtat tagagagaat
     5641 agattttctt aaattaaatt gtgaaataat gaagcaatta gttccagcga gtgttaaggt
     5701 aaaagaacaa aataagagat ggggaagttg tacagctcaa aaaaatattt atataaactc
     5761 aaaaatatct atggcaagat tagatgtaat tgattatgta ataatacatg agtttagcca
     5821 tttagtacat atgaatcact ctaaaaaatt ctatgattta gtaaaatcca ttatgcccga
     5881 ttataaagat aaagaaaatt ggctaaaaat aaatggatat aaaataataa tctgaaaacg
     5941 attattccaa aatataggcg taaagtataa aagaagtatt attgtgaaaa aaataaataa
     6001 tctgaaaatt ttaaaaaaag tcattgacat tgtaaatgat agggaatata atctatatta
     6061 acaacaaaac agaattaaaa tataaaatta aaaaattaaa agcaatgaca gagacaacaa
     6121 tactaatagt gttatccaca gagaaccaat gttgctgaga attggtgtta acagtagtat
     6181 aatgatcact ctggagcttt tgtactgaat atttttagta agtacaaacg tatgtcaacg
     6241 ttacatgact aaggttaagg taattaatta cccgacctga aaaaagaggg gtagatgtat
     6301 tttttatatc tacaaataag gtggtaccgc ggaatataac tttcgtcctt ataagtaata
     6361 tttatattgc ttataaagat gaaagttttt ttattttaag cgatataaat aaatttaatc
     6421 catataaaat taataaaaaa ataataaaaa taaggaggga tttttatgga aaatactgta
     6481 tatgctaaat taaaccaagg aatagattct tttttaagag tggctatgac tctaagaaga
     6541 agagaggttg atatacaatc aataagtatg actgtagata atatgaataa ttcaggtata
     6601 aaattaatag taaatgaaga aaagacatct ttagattctg tcttaaacca tatgaaaaaa
     6661 ctacatgata taagagagat aactgttgaa agaatgagac attaattaaa tatattttat
     6721 aaaactaaag ataaatatgc ttattttaaa ataaaaaact aaagataaat attaaataag
     6781 aaaaatctta gattaaaata taagtgtcat tgaatttatt agtttatcac agttaataaa
     6841 tttattagtt tgttgtagct aataaaaact gaaaattcta aaattataat tgtaagatta
     6901 tataaattta gaatttaaat ctgttgaata atattaaaac aatgataatg agtatgaatt
     6961 tctaataaaa ataaatatca taaaaataag aaaaagtatt gggaggaaat gaaaatggca
     7021 agaatgtatt atgaaaaaga tgtggattta gaggttttaa aaaataaaaa ggttgcagtt
     7081 ttaggttatg gaagtcaagg acatgcacat gcacaaaatc ttagagataa cggtgtacat
     7141 gtaatgatag gtctttatga cggaagtaaa tcagcacaaa aagcaaaaga agatggattt
     7201 gaagttaaga gtgtagcaga agctacaaaa gaaagtgatt tgacaatgat gttaatgcct
     7261 gatgaaaaac aaaagaaagt ttatgaagag agtgtaaagg ataacttaaa agaaggtcag
     7321 acattagctt ttgctcatgg attcaatata cattataatc aagtacaacc accagaattt
     7381 gttgatgttg taatggtagc tcctaaagga cctggtcact tagttagaaa tgtatttaca
     7441 aagggaagtg gagttcctgc actatttgca gtatatcaag accacactaa aaaagctaca
     7501 gagacagttt tagcatatgc aaaagggata ggagcaacaa gagcaggtgt tttagaaact
     7561 acttttaaag aagaaacaga aacagattta tttggagaac aatcagtact ttgtggtgga
     7621 ataagtgaac ttataaaatt gggatataaa acgcttgtag atgcaggtta tcaaaaagaa
     7681 gtagcatatt ttgaatgttt acatgaaatg aaactaatag tcgaccttat atatgaaggt
     7741 ggatttgaaa gaatgagata tagtataagt gacacagcag aatatggaga ttatgtatct
     7801 ggtaaaagag taataactga tgctgcaaaa caaggtatgc aaaatgtttt agaggatata
     7861 caaaatggaa aatttgcaaa agcgtggatt aaagaaaatg aagaaggacg tgaaaacttc
     7921 ctaaaaacta gagaagaaga atataataca gaaattgctg aagttggtag aaatctaaga
     7981 agtatgatgt cattcttaaa ataaatatat attaaatgga ttagtttgag ggaaagagtg
     8041 gtgtatagta gtgcgtatga atggagcaaa ggtaatttta gaatgtttaa agaaagaggg
     8101 aattgacaca atatttggat atcctggtgg agccgtgata cctctttatg atgctctata
     8161 cgattattca gatgatttta aacatataag gacatcccat gaacaaggtt tagttcatgc
     8221 tgcagatggg tacgcaagaa gtactaatac agtaggagtt tgttttacaa catctggtcc
     8281 aggagcaaca aatgccataa ctggaatagc aacagctttt atggattctt cacctatggt
     8341 tgtaatttca gggcaggtgc caactagttt attaggaaaa gattcttttc aagaaattga
     8401 cataactgga gcaacattat ctatgacaaa acataattat ttagttagaa acactaaaga
     8461 attagttcct acaataaaag aagcttttag agttgcaaat tcaggtagaa aaggacctgt
     8521 acttgtagat gttccaaaag atttattttt agcagaaatg gattttagtg gtgaggacta
     8581 tgatttatgt caaatagatg attatatgga ttataagagt gattttgatc tagatgatga
     8641 aactaatatt aagcttttaa atgaagcaat agatatcata aaagaatcta aaaagcctgt
     8701 aatatatgca ggtggaggtg ttaaatcttc agacagtgag gaaattcttg aaaaatttgc
     8761 aactaagata gatacacccg tactaaatac acttatgggg cttggaaata tagatagaaa
     8821 gaatgaacta tctcttggaa tggttggaat gcatggaagt agagaaagta atttagcact
     8881 ttctaattca gatttagtaa tagcgatagg tgcgagattt agtgatagag tgataagtaa
     8941 aagttctgaa tttgctaaaa atgcaaaaat aatacacata gacatagacc catctgaaat
     9001 aagtaaaaat atagaatcta atgtatcttt ggttggagat gtaaagctag tccttagttt
     9061 attaattgaa agggttgaaa gtaagaataa tagcaattgg aaagaagaga taaagagatt
     9121 tagaaagagt gagggtgtac aaacatatga atttcatcca caaaatatac ttaaaaaaat
     9181 aaatgaaaaa tatgaaacat taaagaaacc cactgtggta gtaactgatg tcggtcaaca
     9241 tcaaatgtgg gctgctaagt actggaattt taaaggtaat aaaagtttta taacatcagc
     9301 agggttagga actatgggat ttggtttagg tgcagctatt ggaactaaag ttggtaatgt
     9361 agataaaaat gtggttttag tcactggtga tggaagtttt agaatgaact gtaatgaact
     9421 agctacagtt gcaaattata atgtacctat gcttatttta ctactcaata atagaacatt
     9481 aggaatggta aggcaatggc aaaagttatt ttccaatcaa agatactctc aaactgacat
     9541 taatgaaaat gtggattatg ttaaactcgt aaatgcatat aacattgatg gatataaggt
     9601 atccagtatg gaagaattag gaaaggcgct agatatgata gattttaata aaccagtatt
     9661 tttgcaatgt gatatagata aggattatga tgtttatcct attgtagcac ctaatgatgc
     9721 attggaaaat ttgatatgta actaaaactt atactgaata ttgataaaat tttttattaa
     9781 atttttatta gtattcagtt tttgttgttt agagatagat tggcattttt tatttttgca
     9841 aataaatcaa tgactatata attaagaact tattaa
//
LOCUS       DAGSWI010000145         9707 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.146, whole genome shotgun sequence.
ACCESSION   DAGSWI010000145 DAGSWI010000000
VERSION     DAGSWI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.146"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            417..2495
                     /locus_tag="MJF80_003261"
     CDS             417..2495
                     /locus_tag="MJF80_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBZ0281899.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            2495..3274
                     /locus_tag="MJF80_003262"
     CDS             2495..3274
                     /locus_tag="MJF80_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBZ0281900.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            3267..4076
                     /locus_tag="MJF80_003263"
     CDS             3267..4076
                     /locus_tag="MJF80_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBZ0281901.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            4096..4578
                     /locus_tag="MJF80_003264"
     CDS             4096..4578
                     /locus_tag="MJF80_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBZ0281902.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            4599..5003
                     /locus_tag="MJF80_003265"
     CDS             4599..5003
                     /locus_tag="MJF80_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBZ0281903.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(5159..6319)
                     /locus_tag="MJF80_003266"
     CDS             complement(5159..6319)
                     /locus_tag="MJF80_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBZ0281904.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNQNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            6527..8077
                     /gene="xylB"
                     /locus_tag="MJF80_003267"
     CDS             6527..8077
                     /gene="xylB"
                     /locus_tag="MJF80_003267"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBZ0281905.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            8173..9510
                     /gene="xylA"
                     /locus_tag="MJF80_003268"
     CDS             8173..9510
                     /gene="xylA"
                     /locus_tag="MJF80_003268"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBZ0281906.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNISRINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
BASE COUNT         3692 a         1121 c         1800 g         3094 t
ORIGIN      
        1 tcataaatta tttataatta taaaatccat tgttttgaac aatatctaat atattattat
       61 ctgtatttat aaggtcacta tacatatttt caagtccgat attatctaca taataggcaa
      121 acgttattcc tgtatgtgtt ttaaaaaaac tagatgtata taggtatcat cacagatatt
      181 aaaagtataa ataaaagata ttaagaacga ctttcaagca acatattaga aaattttact
      241 aaaatccgga aagcgattga caaacaaagt caaataaagt atattattgg taagttagtt
      301 taatgtacaa atgaattata tataaaacct aatttataaa taacgatact aaacgacatt
      361 atttaactta agctacacaa agatagaata ttataccatt gatttaggag gtagccgtga
      421 taaaaatatc tgaagactat attgaaaaaa gatttgacaa tgaactattg cgaatagaag
      481 catggggaaa aaactcttta cgtattaggt cgtttgtaga ccaaaatttt gttgatgaaa
      541 actatgctct taacgaaaaa cctaagttaa ataaagatga tattacaatt aataaaaatg
      601 aagatggaag tgcaatcatt aaaaatggaa agataaaagc tgtcttagac catagagata
      661 gaattacttt ttataatgat aaaaatgaaa tattattgaa agaatatatt cgattaagag
      721 ctgtaaaaca tgatgatggt ggagaagatg tagggacaat tgagattacc aaagacttta
      781 actctactct aaagttaaaa tcaagagaat atcgaccaag ctatagtgga gaatttgaag
      841 taactactag atttgaatct gagccctctg aaaagatttt tggaatggga caatatcaac
      901 ataaattttt agatttaaag aatactgttt tggagttagc ccaaagaaat tctcaaatat
      961 cagttccttt ttatatttct agtctaggtt atggactttt atggaataat ccgggaattg
     1021 gaaaagtatc ttttgctaaa aatatgacag aatggaagat gttttctaca aattttatag
     1081 attattggat tacttgtgga gaaagcccaa aagagttaaa taaaaattat tctcaagtta
     1141 ctggaactgt tcctatgatg cctgaaaacc ttttaggact atggcaaagt aaactaagat
     1201 atagaacttc agaagaagtt ttagatgttg tgaaggaata ttctaaaaga ggaattaagc
     1261 tttcatcaat tgcaattgac tattttcatt ggcctaagca aggtgaatat aaatttgact
     1321 tagattattg gaaaaatcct aaagaattag ttaaaaaatt gaaagaagag tattcagtag
     1381 aaccaatagt atctgtatgg cctacagttc aaagtgatgc agaaaactac aatgattatt
     1441 tagaaaatgg ctatttagtc aatgttaatc gtggtgttag aatgacaatg caaattcaag
     1501 gaaatacagt ctttgtagat atgacaaatg aaaatgcaag agaatatgtt tgggatagaa
     1561 tagataaaaa ttataaacaa cttgggattg actattattg gttagatgta gctgaacctg
     1621 gctattctgt gtatgatttt gataactata gatataaaaa aggaaatgtt ttaagttgtg
     1681 gtaatattta tcctattgat tatttaaaaa tgatttatga tggtttacat aatgacatag
     1741 agtctgttgt aactcttgta agaggagctt gggcaggagc tcaaaagtat ggagctttag
     1801 tatggtctgg tgacattgat tctagttttg aagcttttaa taatcaagta aatacagggt
     1861 taaatatggg actggcaggt ataccatggt ggactactga tattggaggt ttccatggag
     1921 gaaatcctaa agacccagaa tttagagaac ttatggtaag atggttccaa tatgctactt
     1981 tttcaccaat tttgagaatg catggagata gattgcctca tagtaaacca ttatcaaata
     2041 aaggtggagg ctcaatggta actggagctc ctaatgaaat atggtcttat ggtgaagaag
     2101 ttgaagtaat tcttacaaaa ttcataaaaa ttagagagag cttaaaaact tatttaaaaa
     2161 aactgatgaa agaagcgcat gaagatggaa caccagttat gagaacttta ttttatgaat
     2221 ttcctgaaga tgataaaact tgggaggtgg ataatacata tatgcttgga gatgaaattt
     2281 tagtagctcc aataatgaat tataaagata gaagtagaaa agtatattta ccaaaaggac
     2341 atacatggga aaatatattt agtggagtta gttatgaagg tgggaaaact tatgaagttg
     2401 aatgcccact ggaagaaatt cctattttcc taaagcaaga ttccagttat aattttaaag
     2461 aactaaaaaa tattttggga gaggtgaaat cataatggag ccacaattat ggcaattatt
     2521 attaatcgta ctatatggat tttttataaa ctatgaaaaa aattcaacaa tgtttggaac
     2581 atatcaacca gtaacagcag gttttattac tggtcttatc ttaggagata taaatacagg
     2641 gttatatatt ggaggtacat tacagctatt atcattaggt attagtaact ttggtggagc
     2701 atcaatacct gattatcaaa cagcaagtat agtagccaca tttataacta ttacgactaa
     2761 gcaagaagca tctgtaggaa tctctatagg tattccagtt gctttattga tggtacaatt
     2821 agatgttctt agaaatacta ttggtatatg gctagttcat aaagcagaag atggagctaa
     2881 aaaaggaaat tataaaaata taacctatat gcaaatgtta ggagtactat taacagcagc
     2941 tacaacaggt attccagttg ctctatctgt aatatttgga ccttctttaa ttaataccat
     3001 tttgaaatat acacctgaat ggttaacagg tggtttaaca gtagctggag gactacttcc
     3061 agcagtaggg ataggattat tacttagata tcttcctgct aaggagtatt ttagttactt
     3121 agtaattggt tttgttttag cagtatatat gaaagtacca ttattaggtg tagctttaat
     3181 tggtggagca attgcactta taatttacaa aaagaatttg gaaaatcaag aacaacaata
     3241 tactgtagta ggaggtatgg acgaagatga gtgataaggt atcattaaat aaatcaaatg
     3301 aaaaggtgaa aaaaagtgtc ttatggcgct ggttttttac tagttctgtt tcttcaaatt
     3361 atgagaagat gcaggccttg gcatactgtt atgcagtact acctttttta aaagtagtat
     3421 acaaagataa tccagaaaag ttacaaagtg ctgtattaaa tcatttacaa ttttttaaca
     3481 ctaatccttg ggttgcccct tatattttgg gtattaatgt agcaatggag gagttatcaa
     3541 gtgaagagac tgaagaggct gttgcttcaa ttaaaacagg cttgatggga ccagtagcag
     3601 gtttaggaga tagtttattt gtcgttattc cgtggacaat ttttggagct atagcagcta
     3661 atatggcgat agatggaagt ccatttggaa tcttactgtg gattgtagtg agtgtagcat
     3721 tgaaattatt aagtatacca ctatttaatg ctggttatac atcaggtact aagctaatat
     3781 cttcaattga aaaaagttta aagattttaa ctgagagtac atctatttta ggtttaatgg
     3841 ttgtaggtgc attaatacct tcagttgtaa aagctaatgt tgctttgaac tttcaacaag
     3901 gtgattttac aatgaaaggt caagaaatat tagaccaaat tatgcctggg ttagttcctg
     3961 caattctagt tgtgattgtt tattgggctc ttaaaaagaa tataaaacct atatacttaa
     4021 ttttaggagt aatggttgtt tcaataattt tatatgcatt aggtatttta aaataaggaa
     4081 aggatgatat tagatatgga aatgaaaggt attaaaaata ttcgtataga tgaccgttta
     4141 atccatggtc aagttgcaac tatgtggagt aataaattgg gtgttacaag attaatggta
     4201 gtaaatgatg cagtagctaa caattctgtt caaaaacaag ttttaagaat ggctacacct
     4261 gcaggtatag cgtcatctat aattacagaa gagactgcta taaaaaatat tactgcagga
     4321 aagtatgaag gacaaaatgt attattaatt gttaagtctc ctgtggacct tattccattt
     4381 gtagaggcag gatttaaaat acataatata aatgtaggaa atatgtcaag tagaaaagat
     4441 acaacagttc taagacctaa tatcagtgtt actgaagaag agaaagaagc atttaaaaga
     4501 cttttagatg aaggaattga aattacaact ataatgacgc ctgatgataa gaaaacttat
     4561 cttgtagaca tcttatagat ttaaagaggt gtattaaatt gaatagtatt attttgatga
     4621 gtcatggcaa tatggctgaa gaagtcttaa attctgcaaa aatgataata ggggattcta
     4681 tagattatcc tactgtaaat atgaaacaag atgatggaat agaaggaaca atagaaaaat
     4741 taaaaaaagc tttagaagtg tacaaggaaa gttcagaaat aattataatg gcagatttac
     4801 taggtggaac tccatgcaat gctgctttat tagtagccaa taatgacagt cgaattaaaa
     4861 ttatttcagg gctaaattta ggtatggtta ttgaatcagc ttttttagat acaaaggaat
     4921 tggcagcaaa attagagaaa ataggtaaag ataatatttc tatggttgaa caaaatgcaa
     4981 gtttattatc tgatgatgag taggaagatt atattaatat ttaaaacgaa atacttatgt
     5041 taatacttaa ttgatataca atttttaaaa aatatagcta atcttgcata aactatataa
     5101 taaatctaat tttagaatgt ataatttaaa gggttatggc tgtatatagt cataaccctt
     5161 atatttcatt aattaaagtt aaattttgta tattcaaaaa gctcttgata tttacggcag
     5221 agccaccata tagagatgcc tcagacccta atgatgaaat ttctatattt attcctttgt
     5281 taaaagagct aactaacata tcttttgtaa tctgtaatat ctcagggata tcactaatta
     5341 tttggctatt taaatatatt atttcaggag cataagttgt aatagcatta tttatagcta
     5401 ttgttaagta actacaaaac tcatgaataa cttttttggc attctggtta tcttcataat
     5461 atagttgctt tactatatca gaatctattt taggaatatt ttctaaagaa gaaagctgtt
     5521 caaatacctt tttttctgaa caatactgtt ctaaacagcc acgatttcca caaggacata
     5581 gtttaccatt aggcatgatg atagtgtgac caatttctcc actcatacca tttctgccac
     5641 tatataattt attatttatt ataatgcctg aaccaaatcc actgtgaata ctgagactaa
     5701 gtagtgagtt atgtacagtt gaaaaagtat tctcagctaa agctgttaag tttgcttcat
     5761 tttctatgtg aataggaaag tcatactttt tgctaaggat gctatataag tcaatctcat
     5821 taagattgta atatggagta aacaatactt tattttcaca agtaatccca tgtatagcca
     5881 aagtaagacc aattacctta taaggagtgt ctattttgga tatgttataa ctatttataa
     5941 tttcatctat caattgtata acattgtctt tacttacttg tatatctgtt aatttcttag
     6001 aatttataat agttccatct aagtaagata gagaagaaaa tatatagtcg tatccaatgt
     6061 ccatacttaa agaaatccct gcacatttat taaatactaa taatatgggt tttcttccgc
     6121 cactatgagt actatttcca attccgattt catgaactag agattcatca ataagttttt
     6181 tggtaatagc ggatatagtt gctttattca atcctatagt agaagctata cttgccctag
     6241 aaataggacc attttttata atttgttcaa gtaccaatct ttcattcatt tctcgaatag
     6301 tgtatttatc agtaaccaat ttgatattcc tccttaaaat aatattgtaa tactttttac
     6361 acaaaaataa aaggttattt tgcattgaca aagataatta aatattttat tattagttca
     6421 taagttagtt taatatacta acaaaaataa agcaagtaaa atatacctaa aatataaaaa
     6481 aattaggata ggaaaacgat agttatgaag tggcattcaa ggagggatga agttggagta
     6541 tgtattaggt gtggatattg gtacatcagg tacgaaaaca gttttatttg ataaactagg
     6601 aaatactata aaaagttgta catatgaata tcctcttata caagagaaat caggttgggc
     6661 agagcaggat gcaaatgatt ggtggaaagc tgtagtcgaa tctataagag aagtagttca
     6721 aagttcaaat ataagcagtg aatgtataaa aggaattgga ttatcaggac agatgcatgg
     6781 tctagttatg ctggacaatg aaggtaaaac attgagaaac tctataatat ggtgtgacca
     6841 acgaacagta aaagagtgtg aggagataac tgatttagta ggtgaagaaa gactgattga
     6901 aataactgct aatccagcac ttacaggatt tacagcatca aagatattat gggttagaaa
     6961 caatgaacca gatatatata aaaatactaa taaaatactt cttccaaaag attatataag
     7021 atacaaattg acaggtgaat atgctacaga ggtatcagat gcaagtggta tgcagttgct
     7081 cgatataaga aaaagagatt ggagcgatga agtactagaa aagttaaata tagataaaaa
     7141 tcttttaggt aaagtttatg aatcacaaga gattacaggt tatgtgacta gagatgtagc
     7201 aagtttgaca gggcttaaag aaggaacaat agtggtagga ggagctggag accaagcagc
     7261 aggagctata ggaaatggaa tagttaaaga tggagtagtt tcatctacta taggtacatc
     7321 aggtgtagta tttgcttata caaaagagcc aaaaattgat aaagaaggta gagttcatac
     7381 tttctgtcat gcaataccaa atacatggca tgttatggga gttactcaag gagctggctt
     7441 gtcattaaaa tggtttaaag ataatttctg tcaaagtgaa gtggaagttt caaattcttt
     7501 aggtgaagat gtatatgaga ttataaataa tcaagtaagt caagtaccaa caggatgtaa
     7561 tggattgctt tacttaccat atatgatggg agaaagaact cctcatcttg acccttatgc
     7621 aagaggtgtg ttctttggat tatctccaat ccattctaaa aaagaaattg caaggtcaat
     7681 aatggaaggt gtttcatata gtcttaaaga ctgtatggat ataatagaga atcttaatat
     7741 agacgttaat gaagtaagag cctcaggtgg aggtggaaaa agtaaagtat ggagacagat
     7801 gcaagcagat atgtttaatc aagatgtata tactataaat tcaagtgaag gaccagcact
     7861 tggagttgca attcttgcat tagtaggagc tggaatatat gaaaatatac aaaaagcatg
     7921 tgatgctata ataaaaactt caactaaatt agaaccaata agtgaaaatg tagatacata
     7981 caatatatat catagattgt ataaaagaat atacaaatct ttgaaagatg actttaagtt
     8041 attagatgaa gtagttaatg caaaaataaa taattaaatt agcttaaaaa gaaaaataaa
     8101 gatagaaaat agttttaaat taagaaaaca tactacatat attatatgtg taaaatatta
     8161 ggaggacatg aaatgagtga aatatttaaa ggaataggac agataaaatt tgaaggagta
     8221 aaatctgaca atgaattagc atttagatac tacaatcctg aacaagtggt aggtaataag
     8281 actatgaaag aacacttaag atttgcaatg agctattggc atactttatg tggagagggt
     8341 aatgacccat ttggagtagg gacagtagag agaccttgga ataatataac agacccaatt
     8401 gagatagcaa agattaaagt tgatgcagga tttgaattta tgtctaagat gggaattgag
     8461 tatttctgtt ttcatgacag agatatagca ccagaaggaa gagatttaga agagacaaat
     8521 aagatattag atgaaatagt tgagtatata aaagttaata tggaaaagac aggtataaag
     8581 ttattatggg gaactgccaa tatgtttgga aatccaagat ttgttcatgg tgcatctaca
     8641 acatgtaatg cagatgtata tgcatatgca gcagctcagg ttaaaaaagc tatggaaata
     8701 actaagtact taggaggaga aaattttgta ttctggggtg gaagagaagg ttatgaaact
     8761 ttacttaaca ctaatacaga acttgaaatg gataactttg caagattctt acaaatggct
     8821 gtagattatg ctaaggaaat aggattcact ggacaattct taatagagcc taagccaaaa
     8881 gagcctacta aacatcaata cgattttgat actgctactg ttttaggatt tttaagaaag
     8941 tataatctgg ataaatactt caaaatgaat atagaagcaa accatgcaac acttgcagga
     9001 catactttcc aacatgaatt aaatatatca agaataaaca atgttttagg tagtatagat
     9061 gcaaatcaag gagacctatt attaggttgg gatacagacc aatttccaac aaatatatat
     9121 gatgcaacac ttgctatgta tgaagtgtta aagcaaggtg gaatagcacc aggaggattt
     9181 aactttgact ctaaagtaag aagagcatct tttgaggtag aagacttatt cctagcttat
     9241 attgcaggaa tggatacatt tgcaaaggga ttattaatag ctcataaatt attagaagat
     9301 gaggtatttg aaaactttac taaagaaaga tatgcaagtt ttagtgaagg tataggtaag
     9361 gatatagtag aaggtaaggt tggatttaaa gagttagaat cttatgcact gcaaatgcca
     9421 gttataaaga ataagtctgg aagacaagaa atgctagaag ctatacttaa cagatatata
     9481 tatgaagtag atacaatttc taataagtaa ttgtgtagta attaaacttg gatgtttaaa
     9541 taaaataaca ggatatgttc ttgtttttat tctaaaacgg acaaagataa gtatttgtaa
     9601 aaattctcat aatcatgata aattaaggat atagacttaa gtataattag tttatatcct
     9661 taaaatttct ataaaaaata atcatttcta tagttatcaa tattttt
//
LOCUS       DAGSWI010000146         9552 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.147, whole genome shotgun sequence.
ACCESSION   DAGSWI010000146 DAGSWI010000000
VERSION     DAGSWI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.147"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            51..938
                     /locus_tag="MJF80_003269"
     CDS             51..938
                     /locus_tag="MJF80_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBZ0281907.1"
                     /translation="MIIRIILFRDVPIYAIFKGTFRNVNLIPFYTIYQFIVDSNLDFM
                     KATVNIIENIGIFIPMGIFLPIVCKNLNKKTIIIVIILVSLAFELTQYIFALGSSDID
                     DVILNSLGGIIGINIYINMNKLFPNDINRFKAIIGTSLVLGVIGLGVISKNYHNLLTF
                     KFRPDRKISKILIEENREIIKDINKDTVDIVGTFESFKKGIITIKAGSNNKVKRPENL
                     IDSDGNIRIYLNENTKLVSYIITKESKMDIVKYEEFDVKNLDLLRRYDTINVWIDKEN
                     QPKDKHGIMASKLLIGLYE"
     gene            1053..1838
                     /locus_tag="MJF80_003270"
     CDS             1053..1838
                     /locus_tag="MJF80_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281908.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            2130..3449
                     /locus_tag="MJF80_003271"
     CDS             2130..3449
                     /locus_tag="MJF80_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBZ0281909.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(3524..3952)
                     /locus_tag="MJF80_003272"
     CDS             complement(3524..3952)
                     /locus_tag="MJF80_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281910.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(4043..5419)
                     /gene="hydG"
                     /locus_tag="MJF80_003273"
     CDS             complement(4043..5419)
                     /gene="hydG"
                     /locus_tag="MJF80_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBZ0281911.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(5420..6505)
                     /gene="hydE"
                     /locus_tag="MJF80_003274"
     CDS             complement(5420..6505)
                     /gene="hydE"
                     /locus_tag="MJF80_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBZ0281912.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(6541..7749)
                     /gene="hydF"
                     /locus_tag="MJF80_003275"
     CDS             complement(6541..7749)
                     /gene="hydF"
                     /locus_tag="MJF80_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBZ0281913.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(7764..8015)
                     /locus_tag="MJF80_003276"
     CDS             complement(7764..8015)
                     /locus_tag="MJF80_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBZ0281914.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(8396..9436)
                     /locus_tag="MJF80_003277"
     CDS             complement(8396..9436)
                     /locus_tag="MJF80_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBZ0281915.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         3398 a         1327 c         1348 g         3479 t
ORIGIN      
        1 aaaaattgtt actgtcaatt atatacataa tatttttggt atatatttta atgattataa
       61 gaataatttt atttagagat gtaccaattt acgctatttt caagggaacg tttaggaatg
      121 taaatttaat tccattttat acaatatatc aatttatagt agatagtaat cttgacttta
      181 tgaaagctac tgtaaatatt atagaaaata taggtatatt tattcctatg ggaatttttt
      241 tacctatagt atgtaaaaat ctaaataaaa aaactataat tatagtaatt atacttgtta
      301 gcctagcatt tgaattaaca caatatattt ttgctcttgg aagtagtgat atagatgatg
      361 ttattttaaa ttcattagga ggcataattg gaattaatat ttacataaac atgaataaat
      421 tatttcccaa tgatattaac agatttaagg ctataatagg tactagttta gttttaggag
      481 ttataggatt aggagttatt tctaaaaatt atcataactt actaactttt aaatttagac
      541 ctgatagaaa aatatcaaaa atattaattg aagaaaatag ggaaataatt aaagatataa
      601 ataaagatac agtagatata gttgggacat ttgagagctt taaaaaagga ataataacta
      661 ttaaagcagg ttctaataac aaagtaaaga gaccagagaa tttaattgat tctgatggaa
      721 atataagaat ttatttaaat gaaaatacaa aattagtatc ttatataatt accaaagaat
      781 caaaaatgga tatagttaaa tatgaggaat ttgatgtgaa gaatttagat ttattaagga
      841 gatatgatac tattaatgtt tggattgata aagaaaatca gccaaaggat aaacatggta
      901 ttatggctag taaattatta attggattgt atgaataaaa tattaaacaa gaataaaaga
      961 ataacaaata taaaaaacga aaaaactatt tctataatta tttttacgtt gtgcataaaa
     1021 taagaacatt agtgaaggag tagagataaa ttatggagat aataaaatat attttgtact
     1081 tttttatata ttcattttta ggatggacag tagaaagtat aggatgttct atagcaagca
     1141 aaagaataat caacagaggt tttttaaatg gtccaatatg ccctgtttat ggttttggag
     1201 cggtaattgt gatatcacta ttgggaagat ttaataatgt agtaatagta tttctattag
     1261 gaatgatttt aactacgata ctagaatatt ttacaggatt tattttagaa accttatttc
     1321 atgctaaatg gtgggattat tctgacagaa aattcaatat aaagggtaga gtatgtttaa
     1381 aaaatgcaat atactttggt gtaatgtctg ttttaataat aagatttata catccattta
     1441 taaaatattt tgtaagtata attccatata gaatattaat ttcaatggct ataattacaa
     1501 cactttggac tatacttgat ttgattgtta ctattataac acttaaaaaa ttagatataa
     1561 agttaaactt attagacgat ataattacag accttaatga tataaatgtg aaattggata
     1621 aatttgatag aggagaaata caagctttat ttaaaacaat aaataacgac gaattagaag
     1681 ttagagaaaa aataaacaaa ataaatagta agttggaccg tatagagtct aacattattt
     1741 tacaaaaaag agttataaaa gcttttccac atataaaaca taaaaaacaa caagagcaac
     1801 tagaacattt taaaaaaatt attaatgaga aaaggtaatt atacaattta atgaagacga
     1861 atagaataat ggtatttata agatgatttt cagaggactc tataaaaata gaatagagtc
     1921 ctttattttt tgaaaaataa tgtttatttg ataaaaataa aatatattgt ttctgaataa
     1981 caaatatttt gaaaattata aaaacatata ttgacaattt gtgtcaaaaa tactaacatt
     2041 aagtatataa agaattttat atgagaatat ctttataaat cgcaataatt ttaaaacata
     2101 aatttattta aagaggggat gatttgccaa tgagcgagat aactagtcaa atgataagta
     2161 cagaagaaaa aaaatatgtt gctaaaactc aaaagatacc ttattatcca gtagcattta
     2221 aatcaggtga tggtgcaatg ctatatgatt atgaaggtaa tgaatatgta gattttttag
     2281 caagtgcagg ctctgcaaat gtaggtcatg gtaataagga aatctcacaa gcagttaaag
     2341 aacaaatgga tgatataaca caatatacac ttgcatattt ccatagtgat cctcctgtaa
     2401 aattagctga gaagcttgtg gaaatagccc ctggagataa tgataaaaaa gtactataca
     2461 gtgcaactgg ttctgcttgt atagatgctg ctataaaatt agcaagaggg tatactggaa
     2521 gaacaaagat aatatctatg tgtgaatctt atcatggaag tacttatgga gctatatcta
     2581 tatctgcttt aagtacaaat atgagaagaa aaatgggtcc attgttacca gaagtttatc
     2641 acttccatta tcctgataaa aatagaacag ctaaagaatg tttagatgag atagaatatg
     2701 cttttgcaca ctatctacca gcagaagagg tagctgcaat atttatagag ccaattgcag
     2761 gagatgcagg aataatagtg cctccagtag agtgggtaca aggtttaagt aaaatatgta
     2821 aagaaaatgg aatattactt gtaagtgatg aaatacaaca aggtatggga agaacaggta
     2881 agtggtttgg tatagaaaat tttggtgttg aagcagacct tatagttctt ggaaaatctg
     2941 ttggaggtgg attacctcta ggagcagttg ttggaagaac agaaataatg caaagtcttg
     3001 atgcaccagc acatttattt actttagctg gaaacacaac agtatgtgtg gctgctttaa
     3061 aatctataga gataattgaa aaagaaaact tacttcaaaa gagtatagaa atgggagatt
     3121 atattaaagc aggatttgaa aaactaaaag aaaaatacga tataattgga gaaataagag
     3181 gcataggatt atctatagga gttgatatag ttaaaggaaa aggttctaat gaaaaacatc
     3241 cagatgctac agctaagata tgttatagat gtatacaaac tggattaata atgatattct
     3301 taggacaatc tactttaaga gtgcaaccac ctctagtaat aactaaggag caagtagata
     3361 aagcaatgaa catcatagat tctgcaatag atgattattt aaatggtaga attggtgatg
     3421 aagtatatga agtaactcaa ggttggtaaa ataaatatta aatgaaaata ggagttatct
     3481 caattttaga aaattatttc tgaaatgaga taactctttt aagttaccca caaataagaa
     3541 aattgtttga ttcattaaag actatatttc ggatgtcgtt tttgtgagta tcagagacag
     3601 tattacgaga ataattacct ttattcgtac tatctgttaa tttagttata attaagatag
     3661 ctaaaagaat aagaattact gtgtataaag caataacata tggttttctt ttatatttgt
     3721 atttgttatt gaaaaaagaa tggccataat tatttttatt tactttagat gtatttgtat
     3781 tctttttctt gttacctttc tttttatcat ccttttttct atttttaggg taaggaatga
     3841 ctttgtgtgt attatcaagt ttgatatttt tatttttttg aatattgttt tccaggctag
     3901 cctttttttc tccaaattga atagttttat tggtattgtt gttatctttc aatgttaatc
     3961 tccctccctt aaactttccc tttagataat attactacta acctatggaa tatacaataa
     4021 ataatatata agtaataaaa ttttataggt ataaatctct ttctccacgc tctaaggcag
     4081 tcatattgtt agatactatg tttcttatat cttctctttc tatcttacct atttcttctg
     4141 cgataagttt tttacccata attttaagtt cttcatctcc ataatcgatt aaaaattcat
     4201 ttaatgtagt aagagcattt ggtgtacata cattatggat ttgtcgactt ttagctaagc
     4261 tcatgaatct atcgccagtt ctacctttac gataacaagc tgtacaataa ctaggtatat
     4321 atccatcagt aatcagttct ttaagcacct caacagggct tctatggtct ccaacttcga
     4381 attgttctgt attgtcacca tcttcatatg ctttatatcc tccaacacct gttagagaac
     4441 ctgcactaat ttgtgataca ccatatttta gtaactcatt tctcatttca gcagtttctc
     4501 tagtggacat tatgattcca gtaaaaggaa cagctaatct agttatagct actatttttt
     4561 tgaacatatc gtcagaaact aagtgaggaa aatcttctaa gctcatacct tcagcttttt
     4621 tcaatctagg gaaagatata gtatggaaac ctacaccaaa tttttcctct aagtgttcgt
     4681 tgtgcatcat aagtcctaga acttcaaatt tagggtcaga taaaccaaat aaaactcctg
     4741 caccaacatc atcaacacca gcttccatag ctctatcaaa agctgttaaa tggtaataat
     4801 agtcattttt tatagattga ccatgcattt ttataaatgt tggtttgtga taagtttctt
     4861 ggaataatat ataagttcct attccttttt cttttagtaa tttatactca tctacacttg
     4921 tagctgctat gttaacgttt acccttctta tatttccgtt ttcattatat gttgaataaa
     4981 ttgcatctaa gcaatctaag atgtaattta tatcacaatt tttagggtct tcaccagctt
     5041 ctagagcaag tcttttatga cccatttttt ctaatatttt aacctcttct tttatctctt
     5101 ccatggtaag tttttttctt ttaaatttgt tgcatttact aaaaccgcaa taaacacatt
     5161 catttacaca gtagtttgat acataaagag gggcaaataa aaccactcta ttaccatata
     5221 tttcattttt tatttgccca gctatactaa ataatctatc tagttgtttt ttatcttcca
     5281 cttcaagaag tgtagctata tctttataac taagtttttc ccttctatca gccttatcaa
     5341 gtaccttttc aatatcatca gaagttgaat tttttgcatc ttctaataaa gaattaataa
     5401 gttcatggtt tatgaacatt tacaaacgcc tccattcaat attatcaccg cgagaatagt
     5461 caacttcaag tcctaatgac ttgatgtttt cttctaaact ttggtgatat tcacaagctt
     5521 cttgaccagt aaaggctttg ttatcgtaaa gagaatattg ttttctaaat tcttgaggag
     5581 aaaggtttgg cataattaca ttgcaaccag ctaaaagacc tgcatttcta ccactagggt
     5641 ttatagatgc caaggctgtt gtagcgggta ataaaacttt tggaagtaga agtcttgtaa
     5701 ttgaaagcat aagtaatgtc ttctctaaat ctccatgttt ataatcctta aaaattgtat
     5761 catggtgtgg aatgaaaggt cctattccaa ccatatgagg atttaactct tttaggtata
     5821 ataaatcacg aacaaggtct tcgttgttct gaaatggtga ttcaaccata aatccagcac
     5881 ctacttgata accaatttct ttaaggtttt ttaggcattc ttttctagtt ctaagctcaa
     5941 tgtttggcgg atgtaatttt ttataatgac tgtctgtagc agtttcatgt cttaaaagat
     6001 atctatctgc accacataaa aagtattttt tatacgaatc ataactcttt tctcctagtg
     6061 atagagttat ggcacaatcc tcatatttat tttttatact tgaaacaatt tcacatattt
     6121 tatcatcagt aaaatatgca tcttcaccac cttgcaaaac gaatgttctg tatcctattt
     6181 tataacctat atcacaacat tcaagtattt catcaagtga aagcctgtat cttatgatat
     6241 ttttattaga agattgtatt ccacaatagt agcaattatt tttacaatag tttgtaagct
     6301 ctataagtcc tctcaagtag actctatttc cataatgttt atttctcatg tcgctagctt
     6361 gtttataaag atagtttctt aaattttgtt cgaagttata ttcctcattg tcgatatttt
     6421 ctattaaata tagtagttca tcaaaactta atgaattaat gctgtaaagt ttatctataa
     6481 agtatttaat cttattttta gtcatattca aatatgtaat tattaagtat agttaaattt
     6541 ttaataattg aaaagttcaa gtgatctttc caaaatacct tttacatatg ctattaagac
     6601 accatagttt acaataggta cattgaaaga ttttgccttt tctattctag ataacattcc
     6661 agctctattc atcatacaag ctccacagtg aactacaagg gcatatttat ttatatcttc
     6721 agtgaaggat actccagaac taaattcaaa agtaatgttt ttgccagttt tctttctaat
     6781 catattagga atttttactg ttccaatatc atcagtttgt ctatgatggg tacatccttc
     6841 ggccatcaag attttatcac catcttttaa attttctaaa gcataagctc cattaatcaa
     6901 ttctttaagg tctccctttt gtctagcaaa caatatagaa aaagatgtaa gaggaatatc
     6961 ttttggtgta tctttatcta cttgtggaaa tacttgagag tcagtaataa caagttttgg
     7021 ttttttgcct aaattagata gagtttcttt taaactatct tcttttgtaa ctatagaaat
     7081 tgcaccatta tccaaaatat ctcttataac ttgctgttga ggaagtataa gtctaccttt
     7141 tggagctgct ttatctattg gaacaactaa aacaactaaa tcatttggtt caattaaatc
     7201 tgataccaat ttgaactcag tgctatcctt aggcaaaact tttattattt cattttttaa
     7261 attttcaatt ccaattttat ctgttgatga gacagaaaca actggacatt taactttatt
     7321 tttagttaag tttaaaattt ctgattgatt cttaatagta tcaattttgt taagaattag
     7381 aatatgaggg atattcttat cattaaattt ttcaattagt gacaaatctt cttgtgatat
     7441 accaatttgg caatccacta ctagtaaagc tatatctgtc ttttctaaaa catctagtga
     7501 tttacctatt ctaagttcac caagttcacc aacatcatca agaccagcag tatctataag
     7561 aacacaaggt cctataggaa ggatttccat tggtctaaat actgggtctg tagtagttcc
     7621 agcaatatct gagactattg cagcactttg atttgttata gcatttatta tgctagattt
     7681 acctgcattt cttttaccaa agagacctat atgtactctt accgattgtg gagtagaatt
     7741 tagactcatt tgtactcacc accttaaact tctttttttg atatactagt ttttacagat
     7801 acatgaggta tgttgcctaa tttaccagtc aaactattaa tagtatccat ttcgccaaca
     7861 actgctatac atacgattga gacaccttct tcttcgcaag gaatgcccat tcttccttta
     7921 ataatacctt taaaacctga tacaacctca ttgaatttaa attgacattc ttttggctct
     7981 tccaaaatag cacttataac tgcaaccttt ttcataaata tcctcctaac tcagaaaata
     8041 tgtcttaaac aaatccaaaa tcaaaaaact tttctttgta aagaaaagtt tctacaaaag
     8101 aaaagttatt aattacacaa gtataatgtg ctatattaaa ctatttcctt acagattaga
     8161 taactctttt ctggtagggt tagcttgtaa aaatttaatt taacaacaag taactgaaga
     8221 aaatttttat acaatttata taaattataa taaaaattaa ctaaagaaaa ttgaagttac
     8281 ttatataata gtagtaaatt gaaattaagt ctatagaaaa accaattttt gtataaaaat
     8341 aaaaattaaa gttaattatt tgttaagaat tgactttggt aaaatatata attaattatt
     8401 ctttaagcat ataatttata agtaatttta gtgtattttc aatagcttct atatgtgttc
     8461 tttcataatg gtgactattg tttgttccag gaccaacaca tgctatttga acatcttctc
     8521 cccaacgaat agcttctgaa gcatctgaac catagaaagt aaatacatcg acagcgtagt
     8581 ctaaattatt gtcttctgca acatcgacaa gcttgtttct taaattaaaa tcatataatg
     8641 ataaattatc ttttgcaact atagttacac catgttcact agatgtttga cttggtccag
     8701 taggtgctat atctattgct acaaattctt ttgttttttc aggtaaacat ttagaaacac
     8761 catgtccaat ttcttcataa ttacttatat aaaagttagt agtatgctta ggtgttaaat
     8821 tgttttcttt aaaatatcta catatttcaa gaaccattgc tacagctgat ttattatcta
     8881 tatatctaga ttttataaaa ccagattctg tagcctcaaa tctatggtct aggcatacaa
     8941 aatccccaac acttattcct aaatctaata catcttgctt tgtaaatact ttttcatcaa
     9001 gtctaataat cattgtttct tcatttcttt cagtggcagc ttttgtttga ccatatatat
     9061 gagtagaagc atacttaaat acaacacttc ctctgatttt tttacctttt ctagttataa
     9121 cagtacagtt ttctccctct atagctgccc aacatccacc accaacttta tggtatttaa
     9181 gagtaccatc agatgatatt tcttttacta tagctccaag tgtatccata tgtgcagata
     9241 ttgttatttg atttgaatca tcttcaccag gaagagtagc tataagtgca cctttttttg
     9301 taagaatagt gtcaagacct aaattttcaa aatctttttt gatttctaaa actgcatttt
     9361 cagtatatcc agctgggctt ggaatgtcca aatatttttt cattaaattt atagtattag
     9421 ttaaatttaa attcatattg tttcctccta aataaatata tttatacaaa ttataacata
     9481 aatacaaatg ttctgaaaac taaaattaaa agaatagaat ctaaacatat ttaaaaaaaa
     9541 agtatataaa at
//
LOCUS       DAGSWI010000147         9531 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.148, whole genome shotgun sequence.
ACCESSION   DAGSWI010000147 DAGSWI010000000
VERSION     DAGSWI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.148"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(230..1600)
                     /locus_tag="MJF80_003278"
     CDS             complement(230..1600)
                     /locus_tag="MJF80_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HBZ0281916.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            1883..1981
                     /locus_tag="MJF80_003279"
                     /pseudo
     CDS             1883..1981
                     /locus_tag="MJF80_003279"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            2000..2421
                     /locus_tag="MJF80_003280"
                     /pseudo
     CDS             2000..2421
                     /locus_tag="MJF80_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2652..2924
                     /locus_tag="MJF80_003281"
     CDS             2652..2924
                     /locus_tag="MJF80_003281"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBZ0281917.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(3287..3367)
                     /locus_tag="MJF80_003282"
                     /pseudo
     CDS             complement(3287..3367)
                     /locus_tag="MJF80_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(3407..4075)
                     /locus_tag="MJF80_003283"
     CDS             complement(3407..4075)
                     /locus_tag="MJF80_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBZ0281918.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(4911..7331)
                     /locus_tag="MJF80_003284"
     CDS             complement(4911..7331)
                     /locus_tag="MJF80_003284"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBZ0281919.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVETFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(7685..8035)
                     /gene="rsfS"
                     /locus_tag="MJF80_003285"
     CDS             complement(7685..8035)
                     /gene="rsfS"
                     /locus_tag="MJF80_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBZ0281920.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(8152..8724)
                     /gene="yqeK"
                     /locus_tag="MJF80_003286"
     CDS             complement(8152..8724)
                     /gene="yqeK"
                     /locus_tag="MJF80_003286"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBZ0281921.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(8725..9414)
                     /locus_tag="MJF80_003287"
     CDS             complement(8725..9414)
                     /locus_tag="MJF80_003287"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBZ0281922.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         3134 a         1669 c         1102 g         3626 t
ORIGIN      
        1 tattactttt tacattataa taatattctt ttaatcaaaa aaacacctac tattaaagta
       61 aatgttttta agtattttta attttaaaat ccatatagtt catatgaaac taaatataat
      121 aactcaacat atgagcaata tcgagacatt tacataccac atagttaata taaaacgaaa
      181 ataaaaaaat taaacttata aaacccttat taatttacat acccactagt tatttatatt
      241 caaaaaaatc ttctccatct gggttataaa ataatattct acttgccaat ccttgttcat
      301 atttataatt ttctttatca cctacataag aaaatgttaa tgtcatgctt cttgatggat
      361 ctaatgaaat tatatctaaa taaatagtac atttttctcc tggctttaat acaccgaaac
      421 tattaaaatc catttttatt ttagcatatt gccccatata taatgttgaa ttagcttctc
      481 cataaacttt taattttgtt acatctaaat tttgtgtact tgtattttct atatttatag
      541 gaactgttgg gtctttatat ttttcagtat aatattcaag gttatcatca taacattcaa
      601 gataacttcc tttatcttgc caaattatgt gctttgaatc aaaatcaatc tttaatccac
      661 atattatatt tttatttgaa gacggaagac taatattatt gatggtattt tcacactcta
      721 gcataatttt atcacttaga tttcctatcc cagtcacttt tgcagcattt ttaagtatat
      781 ctttattact tccaatatca actagtacaa taggattatt tttagccatt ggtgaagcaa
      841 ctaaagcata aactaaatca gtaccactag ctatataaaa ttcttttgcg ccattataga
      901 acttatttac tattttttta ttagtatcat atctatctac tccacctaat ctagttgaat
      961 tagtatcatt aactagtttg tcactcattg atgaagtacc acctattaca taactttcta
     1021 caccagttgt attaaatgga acactttttc catcagttaa tactatagct gctttatctc
     1081 taactgctac aggtgcagca ctcattgcat caggctctcc tttaaaagca ttagtcaata
     1141 taactttatt tactttgtta attgagttta tttcttttgc aacattatag cttgtcttaa
     1201 ttctatcact accttgaagt cttttagcct ctattccttt atcttttaga agagtttctg
     1261 ttgctttatc aatagaactt tctccaccta ttatgtatac ctttttagct ttctctactc
     1321 ttttaagagt tgcattaggt atgttatttt tcttagttaa aaaaattggt gcatttgttg
     1381 ctcctgcaag tccactcgca cttaatccat cagccattgt gctgtctgca ttaattaata
     1441 tagctgtagt ataattttgt tcatcagcta taagagcagc cgtttcatat ttatcttttc
     1501 cttgaatagt ataaattgta tcaagtgcat tagctgatat aggaccagca gcaaacattg
     1561 acacggctaa ccctagtgat aataatttct ttaatttcac aatatctccc ccttttatat
     1621 atgaaatact atatatctta cataattcta cattaaattt tcttttcctc ctttaatgat
     1681 atttatcttc ctgttaattt aagtgtatac aaatacgtgt tataatgtaa ctgtaaagaa
     1741 ttaaaaagat aatgacataa ttagagcagt ccaactgttc ttttataaaa ctaacttata
     1801 aagggagcgt acatatatga atatcaaatc agcttttata agaaaaagat gggaaaaatt
     1861 ctatgtatat gtggaataag aaactggcaa aaagaaacaa aaaagttatg gaagctataa
     1921 aaagaaaaag gatgctgaaa aacatttaat tgaaataaag tctactataa ataataataa
     1981 gtttgttgct ccaaaaggta tgacatttgt agaaagatgt tataaatata tagacatgaa
     2041 taaaaataat tggtctccat atactgttac aaatacaaaa tcttgggtta aaaattacat
     2101 agaacctttt ttaagaatct aaaactcaca gaagtaaatc ctagttcagt acaagctttt
     2161 gtaaattata actttaataa ttctaccttt actagtgcta aagtaaggta tagctttcta
     2221 agttctgtaa taagggaagc gtatagatta agagaaatac aagaaaattc atgtgatttt
     2281 ataaaacttc cttctaaaga aaaatattct acaaatgaaa tatacaatag agaagaagta
     2341 ttattgctaa tagaaaagtt acttggtaat aatatagaaa tacctattct tttaatgtta
     2401 cttctaggtt taagaatgtg agaagctact gggcttaggt ggtcagatgt tgacttagat
     2461 aacaatctga taaatgttaa tcaaattctt gtctattcta atggcaaaat atcttttaaa
     2521 gagccaaaaa caccaaaatc aaaaagagct ttatcagctc caaaagaatt aattgaaaaa
     2581 ttgaaaattg aaaaactaag acaaagcaaa ttaaaattgt aaggtatcct ggaaaacaga
     2641 aataacttag tttgtctaaa caccaattta aatccgtttc taccttcttc actaagaaaa
     2701 acgtttcaca attttacaaa aaaaataatt taaaaaagat tagaatacat gatttaagat
     2761 atacaagtgc cactctgttg cttttagggg gaactaatat gaaagttgtt tctgaaagat
     2821 tgggtcatac agatataaaa ataactatga atagatattc tcatgtactt aaagaaatgg
     2881 acaaagaagc ttccgacaat ctaagcgaac ttctatttaa atagtaaaaa ctgactgtca
     2941 gttaaatgtc agttaaacct aaaaaaatag gttttgtcag ttgattatca gtcaattatt
     3001 ttataatagt ttcacttaaa aacgtctata tctacacatt ttactatttg tttatgctta
     3061 ttaatatcta tctgttatta ttaattctaa tagcttctat ttaattattt agttcagttt
     3121 acaggaaact acacacaaac cataaaatag gatgttgaca atataacaga tagtatgctc
     3181 ctgtaaaagg taacatacta tctgttatgt tgtctggatt tccaaatggt taccctttgg
     3241 cacacgactt tgctatctcc ttggtaaact aaaagtttgt aatctaaaat cctaatccat
     3301 ctctataaaa tttaattgac ttctccatac tttttacacc taaacatata aaagtaattt
     3361 tattcactta atgacctcca taattcccaa attagctatt tggaacttaa ctaaaaaatc
     3421 ctaatataag ggcaactatt ataatggcaa tcggaataca tactgtgtaa tcctgttcca
     3481 tagagtcatc atctgaatgt agttttacaa ttcgcaattt agcaatcagt ttgaagcaaa
     3541 aatagcctat tattgttcca attacgttcg tgagtaagtc gtctatatcg gttgctctgt
     3601 gtaacgtaaa aagctgaata gtttcgataa aaagagataa tccaaaacca atcaagaatg
     3661 tatttctcaa acgttcaaag gttttgctta taagcggaca taaaaaacct aatgggacaa
     3721 aaagtaaaat attcaaaata aaactcaaac taaatccatc actaaaagga ataagattta
     3781 tgtttggatt aaagaacgtt tcccctaact gtgacagtct tatatattca ccgaatgtag
     3841 gtataccaac aatatgtgat agtgttaaac ataagtagta atacattgcc agtgataaaa
     3901 tagatgtttt tacctttgga atatcactat ttttcttgaa tattacaact aagaaaatac
     3961 caatcaagat aattcccaac aagaaaatag gattaattaa caataaatct ttgatagctt
     4021 caaacagact gagttgaatt tctacacctg attgattatt tgttacagtc tccatttgta
     4081 catacctcct cataacattt caattatttc taaaaaacaa aatagaagat aaatcttaaa
     4141 atcatcctat caaaattttt aaatttgcct ttatcagttt ataccaccag ttaattcctg
     4201 tcttacttct gtatggtatt cttgggattc tttcatcaaa ttagacaaaa aatggttacc
     4261 atatcaataa actggttacg ctccatatct cccgaagcaa ccatcccctg agaataaatc
     4321 cgtatcagga atctccacat ctactgcacc tatcacaagt tatttgataa catcctcaga
     4381 caatcccaac ttggatatat cgacaacacc cattagaata ccactgctga ccatacttgt
     4441 tttcctgctg ataattttaa tcaactggga agttgaaacg ctatcttttt tacaagttcc
     4501 tttttagaaa catggtagct attatatgaa tctgaatttc gttgtctggg tgtttcatat
     4561 aaagcataga tatgcacctt ttttgcctgt tttcattata tagaaaaaaa tttcaaaata
     4621 caataattat atttttttcg tttaagctta gagcagttcc ttgatgaaca acactttgga
     4681 aactgaatga tttacatacc acttaataca ttaactcgat ttaacattaa ttataataat
     4741 atttaaaaca acaactaggg tatataagaa tgaacttttt agttctttaa tcattttttt
     4801 gtataaaatc aaatctattt ttataatttc aacaatgaaa aaaggcttat ctctattttg
     4861 agacagcctc atttatttta taaatttata tttttgttca tatttttgag ttatttaaca
     4921 actatattta ctaatttttt aggtacagct acaactttaa ctatagtttt accttcaact
     4981 aatcctttta ttttttcatc ttctaaagct accttttcca tctcatcttt agatatattt
     5041 gagttaacag ttaattttcc tctaactttt ccattaactt gtacaacaac ttctatttca
     5101 tcttttacta aagctttttc gtcatattta ggccagtcta tatcaaatac acttccttct
     5161 ttacctatca tagtccatag ttcttcacct aaatgagggg caaatggtgc aattatagtt
     5221 actatagttt gtacaccttc ttttataact gcttcgttta tattatctaa ctctttatat
     5281 ttatacatat catttattaa ttccataaga gctgatatag cagtattaaa tccgaatttt
     5341 tcacttaaat cagcagtaac ttttttaagt gtagagtgta tagtatatct catatcctta
     5401 tcttgggaat ttaattctcc aaactctaca tcctttttaa ccacatctgc taactcgtca
     5461 accaatctat aaaccctatt taagaatcta aagcatccat caacaccttg ctctgaccag
     5521 tccaaatctc tttctggtgg tgctgcaaat aatacgaaaa gtcttgcagt atctgctcca
     5581 tactcatcta ttatatccat aggagatact gtattccctt tagacttact cattttagaa
     5641 ccatccataa gtaccattcc ttgagtaagt aagtttttaa atggctcatt gaagtctacc
     5701 attcccatac tcttaaatgc ctttacaaac catcttgcat aaagtaaatg cattatagca
     5761 tgttctactc cacctatata ttggtctact ggatgccatc tattaactaa ttccttacta
     5821 aatggttcat tttcattatt gctatccaca tatcttaaga aataccaaga agaatcaacg
     5881 aatgtatcca tagtatcaac ttcccttcta gctggttttc cacagtgagg acaagttgtt
     5941 gacataaatt gctttgaagt cgtaagtgga gattcccctt ttccagtaaa ttcaacatct
     6001 gttggtaaaa gaactggtaa atcttccttt ttaactggaa ctattccaca ttcatcacaa
     6061 taaaccacag gtataggaca accccagtat ctttgtcttg aaactaacca gtctctaagt
     6121 ctataattaa ttgttctctt tcctctacct tctttttcta acttatcgat tattccttca
     6181 aatccttctg aagcatctaa tccattaaat tcttctgaat ttatcatttt gttatcttct
     6241 gttattacat caattatgtc taaattatat ttttcagcaa aatctctatc tctttcatca
     6301 tgtgctggaa ctgccattat agcacccgtt ccatactcaa ctaatacata gttagctatc
     6361 catagaggaa ctttcttgcc atttaaagga tttataacat atctacctat aaacattcct
     6421 tctttttcta catcagctgc tgttctttct atttctgtca tagtatgcat tttttgaaca
     6481 aaagtttcaa catctgcttc atattcagtc cctgcaacca attctttaac taattcatgc
     6541 tctggagcaa gtaccatata agtaactcca taagttgtgt ctggtctagt tgtaaatact
     6601 gtcatagatt tatcagttcc atctatatca aaaactaaat ctgctcctgt acttttacct
     6661 atccaatttt tttgcattgt tttaactttt tctggccatc catctaatgt atctaagtca
     6721 ttaagtaatt cttctgcaaa ttcagtagtc ttaaaatacc attgttctaa atctttcttt
     6781 aatactgttg ctttacatct ctcacatgct ccttgaacaa cttgctcatt tgcaagaaca
     6841 gtctcacaag aaggacacca gttaacatat gattttttct tatatgcaag cccatgctct
     6901 aaaaacttca aaaatatttc ttgtgtaaat ctatagtatt ctggagttga agttgcaact
     6961 tctttatccc aatcataact aatccctaat aaatttaatt gctctttcat ttcttctata
     7021 ttttccattg tccatttatg cggatgtatt ccatgtttta tagctgcatt ttcagctggt
     7081 aatccaaaag aatcccatcc cattggatga agtacattat atccttccat ttttttgaat
     7141 cttgcgacaa catcacctat agaataattt ctaacatgtc ccatgtgtat ttttccagat
     7201 ggataaggga acatttctaa tgtgtaataa ttaggttttt cagtttgtgc agtatccatt
     7261 ttatactgat tattatcttt ccagattttt tgccatttcg attctacttc tttaaaatta
     7321 tatacgctca tattttgcct cctattttta attgtgtata actaccttat ttagtattat
     7381 ttacttataa tttccaaata attctttgaa aataaaaaat cctcgtcact aaacttacgt
     7441 ttagggacga ggataaatct tcgcggtacc accctaatta gcctactaca tatatagact
     7501 ctcttagata gcttttaatc cagccatgga gaatagattt taatctagta agttcaaagt
     7561 tatataatat tagttctcac cttccactaa ctctctgaaa aaatataatc ttttactgct
     7621 ctagtctatt caatttaata taatttttga aattttctaa ttataataac atatattatc
     7681 atgtttatgc taatgtgtca atatctatat aaggtgcatc tttccataat ttttctaaat
     7741 tataaaaccc tctattttcc tcattaaata catgaaccat tatgtctcca taatcaagga
     7801 gtacccaagt ttgagttcct tgtccttctt tacctcttgg ctctaatcct agttttgcta
     7861 attcatcttc cacattgtca gcaatagctt ttacctgtct ctgagaagat gctgtagtaa
     7921 taataaaata atcacacaaa ctagaaactt ttcctatatt tattataact gtatcttgac
     7981 ccagtttatc ttctatggca tcatatgcta tttttgtcat ttgttccact gtcatataaa
     8041 aattcctcac tttcctaatt aaaatacact tagttattgt aacatattaa tacgattttg
     8101 taacatagtc aaatttaaat ttattaatat gtttgccttt atatagcttt tttataataa
     8161 cttttcttta attagatagt ttcttgccat aactgttctt ggatgtatct cttctttttt
     8221 attgatgaca aacattaaag tattattaaa agaagttaaa agtgcttcat caagcttccc
     8281 tccatatgct aattctctta atgtatctac aactgggaat tttcttcctt cttcaattaa
     8341 atcagctatg taaattattt tttctaaaag agacatgtcc tctctccctg ttgtatgata
     8401 tttgattgca tttataatat cttcatcttc tacttcaaat tcatactcgc atatgtacgc
     8461 cccaattaca ctatgagaca aggaacgatt tccatcctct attgggtcta aatatatttc
     8521 atacttattt acataatcct ctatttcctt gtcacttaaa tactttgcac aatcatgaat
     8581 cattcctgct aagtaagctt tttctttgtc acatccataa atctcacata atttttcagc
     8641 acattttgca acattttttg aatggctaag cctccccact ggtagcattt gatttagtct
     8701 ttggttaata ttttctaagt tcatttaatc atctccatat tgatatagtt tattttcata
     8761 taaataattt tctacatttt cgggtactaa gtaacgtatt gacttgcctt catttagctg
     8821 ttcccttata tatgtggacg atatatccag tgatggaaca taaactgtta taatgtcagc
     8881 attatacttc ttagttagtt tttctatagt ctcttgtgac cttaacaaac taattcctgg
     8941 tctagttgcg gctataaaag tagctaactc aaagtttttc tttacatctt tccactcttc
     9001 aacactacaa attgcatctg cacctgttat aaaataaatg tctgcatttt tgtatttttt
     9061 ctttaagtat tttaatgtat ctattgtata agtccttttc tttctttcga tttctacttt
     9121 tgaaactaaa aaatcttcgt tgctttcagt tgctagaagt gtcatattat atctatcata
     9181 cttatctgta gtgatacata atttatgtgg tggatttcct gatggtataa agattatttt
     9241 gtcaatgtca tacttatctc taataaattc tgctgttgct aaatgagcat agtgtatagg
     9301 gtcaaaagta cctcccaata tacctatttt gattttgcct ttatggcgat taaatttctc
     9361 tagtttttca gtatttttca actctgccat cttaactaga ttttcacttc tcatattttg
     9421 tctcctttta atgttataat actattgtac cattattttt tatttcttaa agcttataat
     9481 ttgttttttt gggtatataa ttatatttat atgctaaaaa acacttaaaa a
//
LOCUS       DAGSWI010000148         9488 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.149, whole genome shotgun sequence.
ACCESSION   DAGSWI010000148 DAGSWI010000000
VERSION     DAGSWI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.149"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(262..915)
                     /locus_tag="MJF80_003288"
     CDS             complement(262..915)
                     /locus_tag="MJF80_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0281923.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERVLREHWKTIPIAEMKKEKLL"
     gene            complement(1160..3025)
                     /locus_tag="MJF80_003289"
     CDS             complement(1160..3025)
                     /locus_tag="MJF80_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBZ0281924.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKNADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(3030..5450)
                     /locus_tag="MJF80_003290"
     CDS             complement(3030..5450)
                     /locus_tag="MJF80_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBZ0281925.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5464..6906)
                     /gene="glgA"
                     /locus_tag="MJF80_003291"
     CDS             complement(5464..6906)
                     /gene="glgA"
                     /locus_tag="MJF80_003291"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBZ0281926.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSIN
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6935..8065)
                     /gene="glgD"
                     /locus_tag="MJF80_003292"
     CDS             complement(6935..8065)
                     /gene="glgD"
                     /locus_tag="MJF80_003292"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBZ0281927.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(8067..9227)
                     /locus_tag="MJF80_003293"
     CDS             complement(8067..9227)
                     /locus_tag="MJF80_003293"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBZ0281928.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         3005 a         1718 c          920 g         3845 t
ORIGIN      
        1 tagttggact aaataatttt cctatttttg tattttaatt ttaatataaa atttattaaa
       61 ttttttaatt gataaaataa attctatatt tatgatatta aaattctata agttcattga
      121 tttatatatg ttaatttaaa tacattattc tatcgtagtg ccttagagtt tttatttgta
      181 tttaaagtta aatttattct tgtttttcct ttaatattta atagtaaaac aacaaaatta
      241 taaaaaaaat aaagagaaac tttataaaag cttctctttt ttcatctctg ctataggtat
      301 cgttttccaa tgttctctca aaactctttc aacattttct aaatcattct ccctcataga
      361 ttttactact tcaatatgtt gattattgct gtatgtcaac attttatata actcatcttc
      421 attatcacta gtatatgact ttgacataaa attatatctt aaagagttca atagatttac
      481 taacacctga ttatctgatt tagttatgta tatatcgtga aattcattac ttaattttaa
      541 atagctattg taatttttat actttataga gaccgccatc atttctgtta attcttccat
      601 ttttactagg tctgattcag ttaaattttg taatgctgaa gaaccggcta acgcatccaa
      661 tactccaatt acggaatagg tgtctaattt ctccttttcc tgaaattctt ttactacaaa
      721 tcccttatta ggtatttttt ctagtaaatt ttcacctgct aacctagtta atgcttctct
      781 cgctggtgtt ctactaactc cgatagattt acaaatctca gattcagtaa ttctctgatt
      841 agcttcatat tctccatttt ggattttctt tgatatatag tcatatactt gagttgtaag
      901 tggatctacc ttcataagac gttcctccat attaagatta taaaagttaa atctccctac
      961 aactttggta gagagttcag ttttatctta ctaatttata taatatcaca attatgatat
     1021 tttttatact tctttgtaat attttgaaat actaaaattc ttatgataat ttatacaaaa
     1081 aaagtagaaa aagttttgat aaaacaaaac tctttccaca ttcaccttat tttctattta
     1141 tttttctaaa aaaagatatt taatttactc taaaaattct aaatgattta gagtctaaat
     1201 ctactttcaa ctcattattt tcaatttgta tagtactatt tgcattttta agactatact
     1261 ttataggtat ttcctcaata aaactactct ctatacataa tgatatactt tccacatttt
     1321 cacttctatt tactaaaatt aacattctat cattattttc attaactcta ataaaagaaa
     1381 atacagaatc attattagta tatataaatt ctgtatttcc agaacttaaa agtttattct
     1441 tatttctaat ttttataaca tctctataaa aattaagcat atcttcgtct tcatttttcc
     1501 atggataagt tcttctatta tctgggtcaa cacctccaca aagacctgct tcatctccat
     1561 aatatatata tggtactcct tcaaatgtca tctgtatagc tacagcaagc ttcactaaat
     1621 ctacatcatc atttaattcc gttttagctc ttgtaacatc atgtgttcca attaaattta
     1681 aattagacct aaatgattct tttggataat tttctttaat ctgcatatat ctattacaaa
     1741 gttcatatga attaatttct ccttttaaga aagaaaacat gttatttcta aaaggataac
     1801 ccataacaga atctaattct tcacctaata aataacttct tctctgacca tagctaatct
     1861 tatttgatgc atcttcccat acttcaccta taagaattga gtcatcatct gcatttttta
     1921 attccttttt taactctctt ataaacttag ttggtaattc gtctgcaaca tctagtctcc
     1981 agcccttaat acccatattt acccatttat ttataacgct atcattgtca tatattatat
     2041 aatccatata gctatgttct aattcattta tatttggcaa tgtcttaaca tcccaccaac
     2101 ttttatattt ttgaggaaat tcttcaaaca tataccatga ataatatgga gattctttag
     2161 attgatatgc ccctaaacta ttatagtttc catacatatt aaaatattta ctatctgctc
     2221 cagtatgact aaaaactcca tctaatacta ttgatatacc tttttctttt gctttatcaa
     2281 taagttcttt aaatgtatct tcatctccaa acattgggtc aattttttta tagtcacctg
     2341 tatcatactt atgattacta gaggcttcaa atatagggct tagatatagt atacttacac
     2401 ctaatttttt taaatatcca agtttattga ttatccctct taaatttcca ccatggaaat
     2461 cccatcttat aacatcccct tgactatcct ttatgtacat aggtgtatct tcccaatttc
     2521 catatatgaa actatttttt ttaggattat caaccttacc actacgattt ccattattga
     2581 atctatctac aaatatatga taaagtattc cttctttgta ccatgttggt actttaaaat
     2641 ctttataaac agtgatttga tatttattaa tatcactata attatattca caagaatatc
     2701 cattatctct gttttttcca taaaataaag tctttatttg accatccatt tcaacatcaa
     2761 ctttaaagta ataaaaataa acacctgtac catcaaactt ttcaatctta ccacaaaagc
     2821 actttccaag tttatcatta tctaattctt catttaaagt tattgttctc atttcttctt
     2881 ctctatttat ttctagggaa atagacttga tattgtatcc ctcatttact ttaacacaaa
     2941 tggtcaattc ctcatcaaat tttaaagctc caaacggagc cttaaaattt ttatcccaag
     3001 agttatattc tattacactt tgcatttaat cacacccttt taaaccaaat gtcttttgca
     3061 tactcagaaa ttgttctatc agaagaaaaa aatccagagt tagctatatt aactaaagac
     3121 attttattcc atttttcttt atctctatat aacctgttta tatctgcttg agcttgacca
     3181 taattttcaa aatctctcaa tacaaaatac tcatcattgt aagtagtgag tgaattataa
     3241 atacttcttc cttcttcgcc aagattagga ataaatccat ttattaaatc atctactacc
     3301 ctttttatat ccatattaga atgatatagg tcaagtgaag aatatccacc atatttatta
     3361 taatttagta cttgttctgc actaagtcca aataaaaaca tattttcttt accaacctgt
     3421 tcacatatct ctacatttgc accgtcaagt gtagccaaag ttatagcacc attcatcata
     3481 aacttcatat tacttgttcc agatgcttcc ttagttgtag ttgaaatttg ctcactaaca
     3541 tttgctgctg gtattattat ttctgctaat gatactccat aattctctaa aaatactact
     3601 tttaacttgt cctttactct aacatcatta tttatagtac ttgcaacaga atttataaat
     3661 ttaattatac atttagccaa ataatatcct ggtgctgctt ttgcgccaaa tataaaagtt
     3721 cttgggtcaa gattcaaatt aggattatca agtaattcat gatacatatg aagtacatta
     3781 aatatattca gtaactgtct tttatacgca tgcaatctct taacttgaac atcaaatatc
     3841 gaacttgggt ctacatttaa atcgtattta tcttttataa actttgctag atttgcttta
     3901 ttattgtatt ttatatcatc aagtctttgt aaaacagagg aatcattttt aaatttttca
     3961 atatttttta attgtaatgt atctttcttc catgaatcac caattaaatc tgtaattaaa
     4021 ttgctaagtt gaggatttga acttataagc catcgcctat gtgcaattcc attagtctta
     4081 ttattaaatt tatttggttc atcttgatag aaatctttta atacctctgt ttcaagaata
     4141 tgagtatgaa gcttagcaac tccatttaca ctatgacttg tgacaataca taagtttgcc
     4201 atattgatat tgtcacagtc tattatactc attcttttta ttctatcttg gtcatatcct
     4261 ttattattta attcctctac atatcttcta tttatttctt ctattatcat ataaattctt
     4321 ggtaatagct ctttcatcat acttacaggc catttttcca ttgcttctga cattatagta
     4381 tgattagtat aggacactgt ttttttagtt atttcccatg cttcatccca agataaattt
     4441 tcttcatcaa gcaaaattct cataagttct ggtatgcata aggtaggatg tgtatcattt
     4501 atatgaattg ctaccttatc gaataaatta ttaatattta atttgttctt tttatatttt
     4561 cttattatat cttgtatccc tgcacttaca aaaaagtatt cttgtttcaa cctcaacaac
     4621 ttacctgcat aatttgaatc atcaggatat aatacctgtg atatttcctc agtataatac
     4681 ttatgtttta aagcctcttc atagctacca gaataattta gagcatttga ggtaagttca
     4741 ccaaaatctc tctttggtat ttcactctta aacaatctta aagtatttat acattggttt
     4801 tggtatccga ttattggtat atcataaggc attgccatta caggaaggta gtttttatga
     4861 attactttta aatgactacc ttccttgatt aattctacct caccaccaaa cttcaccatt
     4921 gttgcttcat ttggtcttac agtttcccat gcatatcgtc cttctgtaag ccagttatca
     4981 ggcacttcta cctgataacc atttacaaat ttttgttcaa acagaccata tttatacctt
     5041 attccatatc cttgaccact tatgttcaaa gatgccatag aatctaaaaa gcaagctgct
     5101 aatctaccaa gtccaccatt tcctaaggct gggtctactt cagcttcaat taaatcatcc
     5161 aagttgattc ctaattcaga taaaccttct ctaacttctt cttcaatatt taaatttaat
     5221 aaattagatt ttaactgtct ccctattaaa aattctaaac taaaataata aacttctttt
     5281 ttcttatcta actttgtagc aacccatttt tttgctatct catctttcaa tagactacat
     5341 aatacattta acaattgttc atcagtagct tccttgatag attgtgcata taagctatac
     5401 atttttactt caaatttttg cttgaatttc tttttgctta ttgttaccaa aaaaaccact
     5461 cctttaatca catatgtctc tatatgtttc tatatactcc ttagctgatt ttttccaact
     5521 attatctgtt ttcattgcat tttcaactaa tttaatccat ttttctttgt ccttaaatac
     5581 tttgattgca tttttcaaac agaaaaacat ctcatgagca ttgtaatttt taaaactaaa
     5641 tccattgcct tctccagtaa atttattata aggtgttact gtatctctaa gccctccagt
     5701 ctctctaact attggtaaag aaccatatct catagctaac atttgaccta taccacaagg
     5761 ctcaaataat gatggcatta agaacatatc acttgcagca tatatctgtt gagccaatga
     5821 tgcattaaaa gtaattctag ctgatacttt atctgaaaaa ttagaatcgt aatagttgaa
     5881 cattgattga tattgatgtt ctccagttcc taaaacgaca atctgtaaat cttctctcat
     5941 aagttctggc atcatgtatg ctattaaatc aaatcctttt tgagaaacta atctcgaaac
     6001 tatacctatc aacggaatag atgaatcttt ttttagtccc aatatatctt gtaatctcaa
     6061 tttattttct acttttttgt taattgagtc tacatcataa tgaacaaata tatctttatc
     6121 ttttgcaggg tcatttaaat cataatctat accatttaat atacctttta attttcctga
     6181 ctcttttcta agtaatccat ctaattgttc gccataaaaa gatgtttgta tttcatttgc
     6241 ataagtagga ctaacagtta taattttatc tgcaaaattt ataccagctt tcataaaatt
     6301 tattgagcca taatactcta tatctccatt atcaaagtat ttttgtggta aatccaatat
     6361 atcatataaa acatgcttat caaatacacc ttgatattgg agattatgta tagtatacat
     6421 agtttttata tttttatacc cctcaagggt tgcatatctt tcttttagaa ttaatggtaa
     6481 catccctgta tgccaatcat ttatatgtat tacatctgga taaaaatcaa ttctacttat
     6541 agcttctaaa actgcatctg taaagaaagt atatctttct gcatcatctc catatccata
     6601 gatactcttt ctttcatctt ctcttctaaa ataaaattca ttatctataa agtaaaattt
     6661 tacaccatct aattccattt ctaaaagacc acagtattga tttctccatc caacacgtac
     6721 actaaacaca gctatttctt ttagttgatc ttttaaataa cttggtatag ttgaatattt
     6781 gggcattatt acccttacat ctacaccttc tttttttaat tctttaggca atgcataaga
     6841 aacatctcct aatcctccag tttttgcaaa gggccaacac tctgctgtta cataaaaaac
     6901 cttcattagt cattccccct tctaaaaaat ttatttatat atttgtattc ttttccacta
     6961 caagaggata tttttcatct cctcttaatt ctttcccatt tgatacactc gtatttttat
     7021 ctaatattgc attatatagt tttgcatttg actttattac acaattttgc attactattg
     7081 agttctctat aactgttcct ttttcaacat ttacctttct aaatatgata ctatctttta
     7141 cagtaccttc tattaaacaa ccagatgcta taaagctatt ttctacttta gcactatctg
     7201 tataaattgt aggtgattca ttcttttctt ttgtaaatat ctttctttca gaatataata
     7261 attcatctgc aacttctaaa tttaataaat ctttgctcat ttcaaaatat gattcaacag
     7321 aatttacaca ttttagatat cctgtatatt cataggctcc tattttcatg ttttctgctt
     7381 cttcaataat aaattcttct aaataacaat aattgccttt atttacaatg ttatatatac
     7441 attcaacaaa atcttcacgc ttcataatat atgtctccat acatatgttt gctctaggga
     7501 acttacctaa attatttcct acatttatta ccctattgtt actatctaaa tttagagtta
     7561 agttaccagc aaaatcttta tttgcattat ttacattttt atatattata gttatatcat
     7621 tatgagattt tttatggtat tccaatgctt gagaataatt taaattgcat agcatatagc
     7681 ttggagctat taaaatatat tcttcttcac tcttttctat ataatctaga ttagctaaga
     7741 tagtatatat atcaccttct ctatgtgagt aagtatttct atacttagtg ttttctggac
     7801 taaaaattga taggccacct ttttttctac ttaaatccca atctttacca ctacctatat
     7861 ggtctgttaa tgatctgtat ttttcttttg cgaatattcc aactttagtg attcctgaat
     7921 ttatcatatt agataaagca aagtctatta ttctatatct tccagctatt ggtgtagatg
     7981 ctattggtct tccatagtta agtttgttta ttgctgggtc tccttttttg tttaaattta
     8041 ttatccctaa acattcattt ctcatcttaa agacctcctt ctattaatac gcatctaggt
     8101 tcgatatttt catactcaga aactacattt atttcattat ttacattctt tatctgaaca
     8161 ttatcattta tagttgcaca ctcacctatc atagcttttt ctatagttac attttcacct
     8221 attacaacat ttggcattat tactgaatca attactttac ttcctttacc aacaacaact
     8281 ccatgtgata atacagagtt acctacttca cctaaaattc tacaaccatc agctatcatt
     8341 gatctatgaa catttgcatt tttacctata tattgtggag gcattgccat agtatttgta
     8401 tatatcttcc acttagggtc tgctaaatca agtgtttctg gagattttat tatatccata
     8461 ttagcatccc atagactttg tatagttcct acatctctcc agtatccttt aaatggataa
     8521 gcatacattc caacattatc ttctaacatt ttaggtatta aattttttcc aaaatcatca
     8581 taatttattt ctggattttt ttctgcttcc ttaaaataat ttctcaacat tttccaatca
     8641 aatatgtata ctcccataga tgccaaattg ctcttaggtt cacttggttt ttcttcaaat
     8701 tcatagatac ttgaatcttc atttgtattc attatcccaa atcttgaagc ttcatcccaa
     8761 ggaacttcaa tcactgctat tgtagcttta gagccttttt ccttatgaaa ttttaacatt
     8821 ttgctataat ccatcttgta tatatggtca ccagataata tcaatacata ttctggatta
     8881 taggtatcta caaagtccat attttgatat atcgcatgtg ctgtaccatt ataccaattt
     8941 ccttcttttt cattcataaa tggttgtaaa acatagacac caccatttat tctgtccaaa
     9001 tcccaatgac ttcccatgcc tatatgtgaa tttaatatca acggtctata ttgagttaaa
     9061 acacctacag tatcaatacc agagtttgaa caattgctta acacaaaatc tataattcta
     9121 tacttacctc caaatgatac agctggtttg gcaattcttt ttgtaaaaac acctaatctt
     9181 gaaccttgcc ctcctgccaa aatcatagct aacatctctt ttttcattta atgacactcc
     9241 cctcatctag taattaatat ttataattgc atttttactt ttataaactt tatcagtcaa
     9301 taaatgtcgt gtataaatgt tgcttaaata ataccttaat aaaaataaaa taatatcaac
     9361 tatacaattg atataatgta taccccataa tggacttacc gacgtataca ttattttcca
     9421 taagcactat agtatttatt aatattcaat tatcggttaa aaaataatac aaattttatt
     9481 ttataaat
//
LOCUS       DAGSWI010000149         9389 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.150, whole genome shotgun sequence.
ACCESSION   DAGSWI010000149 DAGSWI010000000
VERSION     DAGSWI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.150"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            955..1329
                     /locus_tag="MJF80_003294"
     CDS             955..1329
                     /locus_tag="MJF80_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0281929.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            1331..2188
                     /locus_tag="MJF80_003295"
     CDS             1331..2188
                     /locus_tag="MJF80_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281930.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            2191..2829
                     /locus_tag="MJF80_003296"
     CDS             2191..2829
                     /locus_tag="MJF80_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBZ0281931.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            3835..4569
                     /locus_tag="MJF80_003297"
     CDS             3835..4569
                     /locus_tag="MJF80_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBZ0281932.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDRLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYN
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            5908..7164
                     /locus_tag="MJF80_003298"
     CDS             5908..7164
                     /locus_tag="MJF80_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBZ0281933.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSEVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            7227..8420
                     /locus_tag="MJF80_003299"
     CDS             7227..8420
                     /locus_tag="MJF80_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBZ0281934.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIAGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            8621..9001
                     /locus_tag="MJF80_003300"
     CDS             8621..9001
                     /locus_tag="MJF80_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0281935.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
BASE COUNT         3487 a          983 c         1523 g         3396 t
ORIGIN      
        1 aacaaaactt taacttgatt tatctataaa tagggtattt aataatattt ttggaaaatc
       61 tttagaaaga attaaaaaga ggagaaaaaa gggagtgcca caaagtgaac tttttagttc
      121 taaggcactc ttttttgtac gaaatcgaat atattttcaa agttttaaaa gtgagaaaga
      181 gacttatctc tagtttgaga tagccaattt taattattaa aattattttt tgttttattt
      241 atcaaagagt attcgtgttt tagaagttag tattttttat tttatatata caaggttttc
      301 cgtttctaat gttttctttt ttaaaataat tagggtattc aattatggta aaaggtggat
      361 ggtattttgc agtgatatta tttaaagcac agtattgaat gttgaaattc tcacatttta
      421 gagtattatc ttttttaatc aaaaaaatct tctatactta tagcaaggtt acttctgcaa
      481 atattccatg catcataata tccaaattta aattttttta atatagagtt tacctctagt
      541 ctacactctt gaataactct ttcctctatc caaattctta taaattcatc ccatttatta
      601 ctatctattt attactatct aaatttttta gtatgtttgt aattagaaat ggatttattc
      661 ctttatgttt tttataaata gtttataact taatttatca tcaaacaaat cattcataca
      721 ggattcctct tttttttaga tattcaattc ttacttttaa taaatattta atacatccta
      781 tcttatgtgg ttttaaaaag tgtttatagc tatcaaaaat ctctaatatt tcattcatat
      841 ttattttgtc atagtaatat tttacatctt ttatattgac aaaaaagtgc tctaagtgta
      901 tagtgtgtat ataacaacat cacactgaaa atgataaaaa gggggtgata aaaaatgaaa
      961 gtgctcattt ctaacacatc agataagcca ttataccaac aaattaaaga gcaaattaaa
     1021 gaagcaatat ttactgaaga actgaaagaa ggggatgcaa tgccatccat aagaaatttt
     1081 gctaatgatt taaaagttag tattttgact attagacgag tgtatgaaga acttgaaaat
     1141 gaaggctttc ttacacgcca agttggaata ggaacattta tttcaactgg aaatttagaa
     1201 atactaagag actcaaagag acgtatagtt gaacaaaaaa tggcagatat gattaaggat
     1261 gcaaaagcac taggaattac acagatagag ttacaagaaa tgatggatat tctatatcag
     1321 gaggattaat atggaaaata ttttagaagt ttataactta aataagactt ataccgattt
     1381 ttcattatct aatataagct ttgcattacc agaagattgt ataactggat ttataggtgt
     1441 taatggagca ggaaaaacaa caacaatccg ttcgatattg gatgtcgtaa agcgtgatag
     1501 tggaaaaata aaaatctttg gtaaagattt cgatggaaat gagaaggaat taaaaaatcg
     1561 aattggagtt gtatttgatg attcatattt ctatgaggag ttgtcaatgg cagaaatgaa
     1621 aagtattatt gcaccttcat atgataattg gtgtgaagaa gattatcaac aaaatatgaa
     1681 gcgttttaat cttaacccaa aacaaaagat tgatacactt tcaaaaggaa tgaaaatgaa
     1741 atttgcttta gcattagcat tatcgcataa agctgagttt ttaattatgg atgagcctac
     1801 aagtggttta gacccactaa tccgtagtca gatacttgac atactaacag gatatatgaa
     1861 gaaatgtggt cgtggtgttt tcttttccac tcatataaca tctgatttag acaaaatagc
     1921 agatatgatt gttatgattg acaatggacg tattatatta gagcgtgaaa aagatactct
     1981 attggacgaa catagaaaag taaaagggga tattcaattt ctaaatgatg aaaccaaaaa
     2041 attattttta aatttagaaa ttacaggtta tggttttata gggattacct caaaaattaa
     2101 tgaagtgcaa aaaaatatgc cagatatttt aatagagcgt cctacaattg aagatattat
     2161 gcttgcatat attgaaagga gagagtaatt atgttgattc atttagttaa aaaagatatt
     2221 ctgattgtaa aaaaatatgt attgttgata ttatttattg caataggaat cccattattt
     2281 atattatggc gtttacctcc atttgcacaa atattgggat ttgtactatc aattatattt
     2341 tcagaattta tgctgtgtca gtatctatta ttaaaggaga gtcaatattc aaaagcatct
     2401 gtattacttt gttcaactcc ttatcctaga cgtggcttag ttcaatctaa gtatgtttta
     2461 tttgcaatgg tatttatcta ttgtacatta gtttactgga ttgaaaattt acttattcca
     2521 caaataggaa cttttgactt gacatatatt ttgattgttt tattaataag ttctattatt
     2581 tatggagttt atatgcctat tcaatataaa ttaggatatg aaacaacaaa attctttttc
     2641 gtggtgataa tcatggcttc atcatttgta ttaccattaa ctatagctca cagcaatttt
     2701 gaaattccat ttatatatca gtatccatcc atgatagtga atattgtatt gttcctttta
     2761 agtttaatta tactgttagc ttcaatgttt atttctatta aaatttttag taataaggag
     2821 cttgtataag gtaaggggga cttttatatg ccacatttgc cagattgggc tgttttactt
     2881 tgtatcttac ttccaatagt atataaagct tgtcagatat ttaaaagtaa aaaatagagt
     2941 acatatttat attgccattt ttattgagta aagttagatg gtatgaaaag gttacttgaa
     3001 aataaagtta cttggtgtgg cagagtaaca tcataatatt atagattata tgtttcttgt
     3061 gaatcaattt gtatttttta atagataata tccagtaatt ttattttcca ttgattattt
     3121 ttcaattatg agtatgattg tgtgtattgt ataccatttt ttaagatgtt tttaaaaaat
     3181 tatatcgtca aaatataaat ctaaggaatc ctataaataa agcttttcaa ttttagaaaa
     3241 ctaataaaaa gtaggaagtt aataaaaaga agctgtctca aaatagagat aagcttcttt
     3301 ttcattgctg aaatgacgaa aatataattg gtttaatata aaaaagagcg ctttatgaac
     3361 tatttatttt gaacagtaga tacacttgca ctcaatttag ttactcctaa gctttatgaa
     3421 ctaaaagttt attttgaagc ctatttttat taaaagagat tttatttttc taaataatca
     3481 tctatataca gtattgcgtt tcttttattt attcataatt tattttctta ttaaagtttt
     3541 tctatattca tattttccat attactttac ttttaatttt atacgaatta tcaaaaataa
     3601 aatagttatt agaaaaaaat aaaagtaatt gcaaagcttg caattactta cttctatatt
     3661 aattactata ccataaaata acaaaaaaat caagtcgagt aatccttcaa cttaagtgat
     3721 atactaggta ttaataattt ttaattaata aaatataact agaggaaaat ctacattttc
     3781 tcaatttctt taattaaatt gacctctaat taagtaatta taaggagaaa aattatgaat
     3841 ttagcaaagg gattagatgt tttaaaaatt tcatcaaatg tattcggtga agataaagtt
     3901 atgtatattc ctgtaatcta cacagaagat gatgcaacac ttattgacac aggtcttccg
     3961 ggtcaaggtg atttaataat tgatgctctt aataaaagta acacgagctt tgataggtta
     4021 aaaaatataa taattactca tcatgatata gaccatatag gaaacataaa ttatctaaga
     4081 gaaaaatcta agaataatat caaagtatat gcatacaaaa gtgaagtcag ctatataaca
     4141 ggagaagaaa ctccttttaa actttatatg ttggaacaaa tggtagataa aattgatgat
     4201 aagatgctta gtatgctaaa tgttatggga cttggattta aatcatcata tactaaagta
     4261 gatgtgtcat tagataacca tgaaaaattg aatctaggtg aggaaataga agttatacat
     4321 acaggaggtc acactagagg tcacatatgt ctttatttaa aagagtctaa agtacttatt
     4381 gctggagatt tgttacaggt agaaaatgga gaacttaagc ctgttgatgt gatgtacagc
     4441 aacaaacaag agttaaaaga tgctattaaa aatataagta attataatat agaaacgata
     4501 gtttttagtc atggtggatt atatcaaaga aacataattg gaacattgaa aaacctaatt
     4561 attgagtagt actatatgat ttacaataat ttcttaaatt acttaaaatt atatctaaag
     4621 atgtagaatc aaaattaaat ataagtaata agaattttgc tttaaaatta tacttaagtg
     4681 ttaagggtga tatgtagtat tacaagtatt ttagaatata gttttttata gtaattttaa
     4741 ggttctacaa tacttcagtt gaaaaatatt taacagcata gtataatggg aaagaaaata
     4801 ctttaaataa actttagtat acaaagtgaa gaaaaatatt taaattatat ataatattgt
     4861 agagtgaggt aaaatattta agttatatat cataatacag agtgaagaaa aatatttaga
     4921 taatgtacca taatacaaag tgagtaaaaa tatttaaatc atgtatcata atgcaaagcg
     4981 aagaaaaata tttaaataat gtaccataat gcagagtgag taaaaatatt taaataatgt
     5041 gttataatac aaagcgaata agattattta aagcatgtat tataatataa agtgcgtaaa
     5101 aatatttaaa tcatgtatca taatacaaag taaaaaagat tatttaaacc atatatcata
     5161 atgcaaagtg aagaaaaatg tttaaattat gtatcataat ataagcagag tgaagaaaaa
     5221 aatttaaata atatatcata atgtagagtg agtaaaaata tttaaatcat gtatcataat
     5281 ataagcagag tgaagaaaat atttaaatca tgtatcataa tataagcaga gtgaagaaaa
     5341 atatttaaat aatatatcat aatgtggagt gaggaaaaat atttaagtta tgtatcataa
     5401 tacaaagtaa agaaaatcat ttaaattata tatcataaaa ataatatgaa atattgaaca
     5461 ctcataatat tagagacttt aatattttaa ttgataggtt atattattta taacattaaa
     5521 aatacaattt acataaaata gacatatgaa aaataaaaaa ttatataata attatgggaa
     5581 tgaggttctc ccttggatga taatatccta aactgccaat gagctgatga cttctatgat
     5641 atcatagatg ccaccagctt tttttgttgt ttaaatcaat aaaatggagg tgaagtactt
     5701 actaaatttt tattttttaa atttgaagaa atatttaatt aatagaaaaa atagtagatt
     5761 atatgttaat tattttatta agaatgggtt aagagtgtac ctatatccta tcaatttaaa
     5821 aaacaattat gatagtcatt acattgacta cagttgatgc aataaaactt tcaaaaaatc
     5881 ttatgatgca aatagaaagg aattaaaatg atagaaaaat ttaaaacttt aatatggatt
     5941 aaaatgataa taagaattaa aagcgtttat aaaacatatg gtggactatt ttttttagga
     6001 ctcataggta taccagttgg tgcaattata ggtcttatag acaccatatt tggaacggtt
     6061 cttctaaaag ttacagatat tcgagaaaca tatccaatgt atctgatacc atttttagca
     6121 gtagtaggag ttgttattgc ttattgctat tttaaatttg gaggaaaaag tagtaaagga
     6181 atgaatctaa tatttgaggt agggcatgga gaagaagaaa ttattccttt aagattggtt
     6241 ccatttataa tatcaggtac atggttaacc catttatttg gtggtagtgc aggacgagaa
     6301 ggagttgctg ttcagattgg tgcaactttt tcacattggg taggtaaaag gttgcctata
     6361 aaaaatgctt ctagtatttt tctagtaact ggtatggcag ctggttttgc aggattattt
     6421 gagacaccaa tagcagcaat actatttgct atggaggttt tggttgctgg ttcactggag
     6481 tatcaatcgc tttttcctgc gtttactgct tcttttacag caagtgcagt atcaaaagct
     6541 ttagggttag agaaattttc ttttgcactt tcttctgaag ttgtatttga cttgtcaata
     6601 ttttggaagc ttattgtttt aggaattatt tttggaatgg ttggtggagc atttgcatgg
     6661 tgtctgaaat tgtcaaaaag aaaaataggg aatagattaa aaaatcctat gataaggatt
     6721 gctattattg gagtatgtct tagtgtatta ttccttttat tttataaagg aagatattca
     6781 ggtttaggga caaatttaat tcaaaatagt ttctatggtg gagaaattta ttcatttgac
     6841 tggctgttaa agtttatact tacaatactg actttgtcag caggttttca aggaggagag
     6901 gttacacctt tgttttccat tggagctagt cttggtgtat tgttagcagg attttttaat
     6961 ttgccaattg aattagttgc agcgcttggc tatgctagtg tctttggtag tgctacaaat
     7021 actttttttg cacctgtatt tataggggca gaagtttttg ggtacagcta tttaccttat
     7081 ttctttgtag tatgcgctat ttcatatata tttaatatgg ataaatctat ttattcacta
     7141 caaaaaattt caacaaaaca ataaataaaa caacataatg tatgattaga agagaaagaa
     7201 tattagagaa aggatagggt aggaatatgt tgacaagttt agctttgatt tttttattag
     7261 gaatggcctc gggaggtatt tttaagagga taaagttacc taacttattg ggaatgttac
     7321 ttacaggaat tatattaggt ccatatgttt taaatttgat tgataattca atactagata
     7381 tttcttctga ccttagaaag attgcattga ttattatatt aacaagggct ggattatctt
     7441 tagatataaa cgatttgaag aaagtgggaa gacctgctgt acttatgtgc tttattccag
     7501 ctacttttga aattatcgga atgatagttt tagcaccaaa gttactaggc gtttctatat
     7561 tggaagcagc agtcatgggt gcagtagtag gagcagtttc tcccgcaatt attgtaccta
     7621 aaatgcttaa attaatggaa gaaggttatg gtacagaaaa aagtattcca cagatgcttt
     7681 tagcaggaac ttctatagac gatattttcg taattgtgat gtttacagta ttcacagggt
     7741 tggcacaagg aaatagtata tcagctatta gttttttaca aattccagta tcaattatat
     7801 taggtgtaat agctggagca gtgattggaa tctgtttagc agtatttttt aagaaggttc
     7861 atatgagaga ttctgctaaa ggggtgttgc ttttaagtat ctcattttta atgataagtt
     7921 tagagactgc tttagagggt attgtgccat tttctggatt attagcagtt atgagtattg
     7981 ggattttcct tcagataaaa tatcgtgtag ttgcaagaag attgtctata aagtattcga
     8041 aactatgggt tggtgctgaa attctcttgt ttgttttggt aggggcaaca gtagatataa
     8101 gttatgcatt taaagcagga attggagctg taattttaat atttggagtt cttttattcc
     8161 gtatggtagg agtattcttc tgccttatta agacaaattt gacaataaag gaaaggttgt
     8221 tctgtatgat tggctatatt ccaaaggcta cagtacaggc agcaatagga ggtgtaccac
     8281 ttgcaatggg gatggcatct ggacaactta ttttgacgtt agcagttcta gctatactta
     8341 taactgcacc tcttggagct tttggaattg atgtaactta taaaaaatta ttaacttctg
     8401 taaaaggcga gagtaagtaa aaataaagat tattttaact ggtaagttat attttggaaa
     8461 aggattatac tattgaatac aatataaaaa aatcatttgg gagattaaga aggtagaagt
     8521 taagaacaga ttttaaggta tgttatgatt attagaattg attttgtgaa attattattg
     8581 taatatttaa ttatatagat aaagaagcgg agtgataact atgtgtttaa aaaaattatc
     8641 aaaattagag ttagttatta tgaaatttat ttggaattta gatataaaaa ccaattcata
     8701 tgagattatt gattatatga aagaagaaca taatttacca gaaaaagtag ctttaaaaac
     8761 tttatcaaaa ctatcaaaga aaagattctt atatgttcaa gaaacaggca agtgtatgta
     8821 ttatacagtt gcaataaaag aaaaggcata tttagagttt atatcaagaa atgtgcaaaa
     8881 tcttttgaag aataatttta taaggaattt attagtatca tttcacgaag aggagctaac
     8941 agaggaaaag ataaagtcgt tagaaaactg ggtagtaaat tgggaagaag cttatgtata
     9001 aatgttatta tttttattac aatttagata attttggttt tgtaagctct gcaactattt
     9061 ttagatggtt gcagagttta tttttatttt ttattccctt aagcaatact taaaagtaaa
     9121 cgcatttata tttactaact tcaattactt ctaatactta aattactttt taaaaatatt
     9181 ttgatatgtc atctctatag taatctagaa cttgtttcta ttctttatat aaagttttta
     9241 tgaatgatat atataatatt cgttcatttg attaaaaata acaaaatatt aaataattct
     9301 actctgatag tttgttaaaa aaataataaa aaatattaat aaacaaaaaa aaattatctt
     9361 aagagaggag aatgttctaa aatataaaa
//
LOCUS       DAGSWI010000150         9243 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.151, whole genome shotgun sequence.
ACCESSION   DAGSWI010000150 DAGSWI010000000
VERSION     DAGSWI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.151"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            191..574
                     /locus_tag="MJF80_003301"
     CDS             191..574
                     /locus_tag="MJF80_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281936.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
     gene            1082..1465
                     /locus_tag="MJF80_003302"
     CDS             1082..1465
                     /locus_tag="MJF80_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBZ0281937.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1467..1817
                     /locus_tag="MJF80_003303"
     CDS             1467..1817
                     /locus_tag="MJF80_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281938.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1918..2376)
                     /locus_tag="MJF80_003304"
     CDS             complement(1918..2376)
                     /locus_tag="MJF80_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281939.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLKEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2421..3209)
                     /locus_tag="MJF80_003305"
     CDS             complement(2421..3209)
                     /locus_tag="MJF80_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBZ0281940.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3348..3785)
                     /locus_tag="MJF80_003306"
     CDS             complement(3348..3785)
                     /locus_tag="MJF80_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBZ0281941.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            4607..5116
                     /locus_tag="MJF80_003307"
     CDS             4607..5116
                     /locus_tag="MJF80_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281942.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVELEVDSLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIGFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFVTRKKI"
     gene            5143..5292
                     /locus_tag="MJF80_003308"
                     /pseudo
     CDS             5143..5292
                     /locus_tag="MJF80_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            5521..6057
                     /locus_tag="MJF80_003309"
     CDS             5521..6057
                     /locus_tag="MJF80_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBZ0281943.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKI"
     gene            complement(6491..7291)
                     /locus_tag="MJF80_003310"
     CDS             complement(6491..7291)
                     /locus_tag="MJF80_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281944.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(7486..9084)
                     /locus_tag="MJF80_003311"
     CDS             complement(7486..9084)
                     /locus_tag="MJF80_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBZ0281945.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
BASE COUNT         3328 a         1357 c         1188 g         3370 t
ORIGIN      
        1 accttgaaaa tagaacctta tttgccattt ataaattata taaaaaggga ttatataaag
       61 tcatatatga aaaaactata ttatactatc ttttcttttt ggaataaact atactttaaa
      121 tatagatatt taatttaaat aaattttaca gtattaatag taaattatct tagaaaggaa
      181 tgtgctttat atgtctacag acaaagttgt tggaatcata gatgaagaaa cggcagaact
      241 tgcaggaatt gaatatactg gtaaaattta tgcttcttct ggtgttataa aacatataaa
      301 gaaaaaacat agatgtcaac tgagtaagga tatttttaat gatataatag atactataaa
      361 aatggtatta aagtcacctg aatatatagg aagtcatcct aaaaaacctg gaaaaagtgt
      421 ggagtttata aaaaaaattg atgattatat tttagtagct acagaattag atacaaaaaa
      481 aggatattta tatgtatctt caatgtatgc tataaaagaa gcaaaattag aaagtagagt
      541 aaacagtgga agagtaaaac aatatgatgg ttaacataat attattaatt ttccctaaaa
      601 tattgaaaaa tattattata taagttataa tattaatata cacgtagata tttattagtt
      661 agatttgagg ttggaaaagg cacccaatac gctactatag agtagttaat agagatgcag
      721 gatacgccgc cctgccaaat ctaattaaat taaaaagaga ggattaattc ctctcttatt
      781 gtttttttga gatactaatt taatctataa tagaaagatt gaaggtcgtt tatacaaaaa
      841 aattttcagt tatatacatt aaactaatta aataagatat ttataatcta tatgtgatac
      901 atattatata ttaataaaag aaaaatatat agaaaatcct tataaaaatt taaattgtaa
      961 tgaataggaa gtaattaaaa agaattttta ttgattttta aaaatttagt tagaaactat
     1021 atcattatgg taaaaaatgt attataatgt ttagaaatat tataataagg agcgattaaa
     1081 catgaatcta tcgaagaaaa tagaagataa aagaaaagct aaacagagag ctgaaaaagt
     1141 taagaaagct aaaattgcta cagctggtgt ggtattagga gctgttactg gtgcagtgag
     1201 tggagtactt ttggctccta aatctggaaa agaaacaaga gaggatataa aagatgcttc
     1261 acaacaaatt gctgaaaaga taaatatgaa aactgtagat gtaaaaggca aagtatctga
     1321 gaagctagag gataaaaaag gaaactttat agaatcaaag aaaaaaataa aaaaatatat
     1381 agataacaaa aagagtgttg aaaattctag tgaggaagat aaaataatag atatagttga
     1441 accagttagt gttactgagg aataatatgg acaatacaat aacgattaac ataagtataa
     1501 tatatgtatt attagctata cttgcttgta ttgctttagt ttatttaatt attgtacttg
     1561 ttaaactaca taagatttta aataatgttg gaactatgtt aaatgaaaat actcaaaata
     1621 taaatacaac tttaaataat cttcctaatg ttgtagaaac ttttggagaa gtaggagaaa
     1681 atatgaaaga tgtaacagaa gtagttactg aagttacagc agattttctt gttactaaag
     1741 acagtgtcaa atcaaatatg gatgttatag ttgatatatt gactataatt aaaaacattt
     1801 ttctagataa aaaataataa gttaatataa aataagccta taagatataa tttaaacgac
     1861 cttaaaatag catttataag gtcgtttttt attagcttga tttgacacta tgtgttttta
     1921 ataattggta atattcctct ttatcatttg aacatatata tctatgcttg ttgtttcaat
     1981 tacatccata agaacatctt tacattcaag ctcaaaatct tccattcctt ctaaatattc
     2041 atcatcttca tcaaaaaaga tataagaatt atatagccat tcttcaaatt tttcttcaag
     2101 tatatcccaa tacgaactcc aaaataatcc acaacctgat atataagatg ctctactttg
     2161 atttatataa acctccttta gaagcttttg taatctactg ctataaagaa aatctgctct
     2221 tccactgctt gatattaatt catttaaatc ttctattgac attaattcaa ctttaccact
     2281 tttatcataa ggagtataat tttcatcaac ccatttatgt ggtttgtttt ctctgcatac
     2341 tgttgctatc aactctacaa cttttgttct tatcattttt ttatcctaac ttatttattt
     2401 tttataaaat aatcatgatt ttataattta tctatttctt gtaatgctaa tttaacagtt
     2461 tcatatctat cagcaccatt tattactaca tcacctttta caagtttatt agcttctcca
     2521 cctacaacat aaaccttttt acctaaacct tgcttataac tagacgcctc aattattaaa
     2581 gaatctttca attgccaatg taaaatctga gcagccactt tatcagcatc acctgtataa
     2641 acgattacat tttcatacac tttattttcc cctccccctt gagaatttga tatagatttg
     2701 tttagtatac cttctgctat caatttagca actttatctt tattattaac ataataatct
     2761 gtatctgctt tactatctac aaaacatact tctattaata ttgctggtgc aatagtatga
     2821 ttaagccaat aaagacctct aacatcagac tttgcacccc tatctttgaa tactgtagct
     2881 aacctagtat tcactctttc tgcataggtt ttgccattat tagtcttata tattgtttct
     2941 gtccctactg gagttgaagt tgtagcattt gaattaaaat gaatctgaac agccaaatca
     3001 gtttcctgtt tatttgctat agcacattgg tctgctaaat gattacttga ttcatcaact
     3061 cttcctgtat aaacagtagc tccacctatt ttaagccatt taacaattaa gtcattcagt
     3121 attcttgttt ctttgctttc gtttatatac ccaacagctc ctgtaccttt tccaattaaa
     3181 gtgtgccctg gtattattac tactttcaaa ctaacatctc ctttacaatt aaatttaact
     3241 tctatttaat atatgcataa cctagaattt tgtttctttt tatatcttat acttttatta
     3301 taacccattt tgtaaaaata tagaatatta agtaaataat gttaaaatta taattttgta
     3361 tataagacat tacttgattg caataatttt atagcatatt catctattct atatccagtt
     3421 ctataatata tatgtttaat tccagcttga attatagcct tagtacaatt taagcaagga
     3481 aagtgagtta cataaataga gcaatcttta actgaaatac cttcttttgc acaataataa
     3541 agggcatttt gttcagcgtg aatggttcta atacaatgtc catctctcat ttcatgacca
     3601 acttcactac aatgcttatc tccagaaata ctaccattat atccagtaga cactattctg
     3661 ttttctgaat taacaattat agctcctaca taagctctat cacaagttcc tctttcagct
     3721 actgtttcgc acagtctcat aaaatattct tgccatgaac atctattttc aagtttttta
     3781 ttcaaatcat atacctccaa aatttatatc taatatataa gatttatacc ttaaaaataa
     3841 tattaaaaac aaatttatac gataaataaa atttattaat actgttttta gtctaaatta
     3901 tttcatttta aattaaagtt acaaggaatc taaaattata tatatctttc ttcgtaatat
     3961 gaatgttaca atataaaaaa ctgtctcaat aaaatagaga cagttttaca gtcaaagttt
     4021 ataatacaag ttcatatttt tctttaaatt cttttacagc ttcgggccat gccttttgtt
     4081 ctgtaatacc tttttcttta gctattttaa ttgcaatctc tcttatcatt gcagcgcctt
     4141 ctaatattcc catttttaat ctcccttcaa atatcttttt gtatagaata taaatagtgc
     4201 taagatttat tacatgctgc atatacaata tctatacaac acataacttg taaattcctt
     4261 ttattttcat acgtctttaa ccgacatata attatattca ataagtttta aaattatgaa
     4321 ctcaattata attattttta tttttttaga atctaaacac taaaaattgt aggtaaattt
     4381 gtcttatgcc tttataaata tttctacagt tttaattact ggacatatct atttttatta
     4441 taaaaagcgc ttaaaaatcg agaatatccg cataaaaact gtatataagc aaaacggtta
     4501 aaggaaaaag aagaaattta cttgaatgtt aaaatgcgtt gcttgaagca atgggcagtt
     4561 ttgcctataa tatcggtaac aactgaaaga aaggtggtta gccctaatgt taagcgaaaa
     4621 cattaaaaca atcaggaagt caaaaggact ttcacaacaa gaacttgcta taaaactgaa
     4681 tgtagtgcga caaacaatat ctaaatggga acaaggactg tcagttcccg attctgatat
     4741 gttgatttcc atatcagagg tgcttgaaac gcccgtaagc actttacttg gagaaactat
     4801 tgttgagttg gaagttgata gcttaaaagt gatttctgaa aaattagaga ttataaactt
     4861 gcaacttgca caaagaaaaa acatgagaag aaaaatgttt cattggttat ttattttatt
     4921 atgtgcagtc atattaatag gttttgtggt cttaggggga ctaaatagtc cttacttgga
     4981 ttggaattat accgaccctg aaaccgctgt ttttggagta gcttttcatg cttttgagtg
     5041 gctatttgtc agattagtac cgattgtcct tataggagca attattggaa tctttgtgac
     5101 acgtaagaaa atatgaagta actctgcttt ttataataca gcaagcgttc cttaaaagcg
     5161 tcattctaca tggcaatcga ggacgactat gtaaggaaga acctttttga tttccaacta
     5221 agcgaagtta ttgaggacga cagtaggctc aaaaccgcat taagtaaagt acaggaagca
     5281 agactgctgg acttatactc tcatgcgtca gatgcaggaa atgcgaggtc tggtagcatg
     5341 atttacaacg atttgtacaa cgactacgag tagaaatata tggagataag tattatctac
     5401 gaaatcagaa aagaaatgac ttgataattt acccatgtta taattattga tgtgaaatta
     5461 ataaatcgaa attccttttt tagatttaac aatactgagt aaggaaaaag tgaatgactt
     5521 ttgcaaggag gtgttaatat caggcaagta aagtcttttg tttttccagt tatatcctta
     5581 atattcttag accaaattag caaagttctt ataggattat tcttaatgga ctttgaaatt
     5641 gatataattg ggaaattttt aagattcaat cctgttcaaa atacaaatct ctcttatggg
     5701 ggaaacttta ttggtattct atctaattta tgggtattgg tattgtttaa cattttagtt
     5761 atattagtta ttatatctgg atatgctttt tataaatcaa aaaatgaaca aacaagctat
     5821 tcagtaaaag taattatgtc ttgtggactt gctggtacaa tatgtagctt gatagataaa
     5881 ttattttggg gaggaagttt agattttttg cagataccaa gcttttttat tttcgactta
     5941 aaagactgct accttactgt tgcagaaata atatttgttg tcataggaat tttgcataat
     6001 agagaaatat caatgaaaga atacatatat ttttgctatc gtcagtttaa aatataaatt
     6061 tagaagataa atttaagttt caaggtaaac ttatttaatc acagcaaata gttggcttac
     6121 ttaagctttt gggctaaact gtgagcacta attaagtctc tgctatctaa caaacggagc
     6181 acaaaaataa aaattaaata ttaaatataa agcacagtta attttgatta aaaagttgac
     6241 tgtcttttct tttgtagaaa tttaagcata ggtaataaag aaatgactgc atttaagcat
     6301 tatgtatata attggaggca ataaaaagcc agcaaagccc ataaaataga gctgtgctag
     6361 caacatgatg agttataaga agataatcaa aaacttacgc tttattttaa tttaaaaaaa
     6421 tgattactgt aaagaaatca ttttttatta taatcactga tacatcaaat attctaaata
     6481 aaatttttat ttatctggaa ttgtatattt tttctataat attaactaca gcgtacattg
     6541 cccaagcaca gatagccaat acaaatactc ccatcattac taaatctaat ttaaatactt
     6601 gagaaccata tactatcaag tagcctattc catatcttga aactaaaaat tctccaacta
     6661 ttacacctac ccatgccata ccaatattaa ttttagttaa atttattaaa tttcctgtgt
     6721 ttgcaggtaa tatgagttta aataatattt gagatttagt agctccaaag ctttttaaca
     6781 tcttaatttt ttcttcatct atattcataa aataattata agctgaaagt atagtaacaa
     6841 caacagaaat cgttacagct gttacgacga ttccttctat tccagcacct acccaaacta
     6901 taattatagg agctaaagca gtcttaggaa gagcatttaa aactaccaaa aatgggtcta
     6961 atatttttga taatttttct gaccaccaaa gagcaattgc taccaaaata cctaatacag
     7021 tacctataac aagaccaaga acagtttcat atacagatat tccaatatgt ttaaataatt
     7081 gaccactaaa tgcatattga ataaataaat tgtaaatatc acttggtttg ctaaatagaa
     7141 atgtatttat aacatttaag tcagctaaaa gttcccagat tacaataaaa ccaattaaaa
     7201 ttaatagctg gtagaataat acatttcttt tttctctttt tacaccttta acatagttta
     7261 tatatccttc agatctttta ttaagcctca tgtgtatcta gctccttcca taatatgtcg
     7321 aaatactttt gaaaatcagg aactttacgt gttttgagag gcgtttttgt tccatccatg
     7381 ttaagttcaa tatcgtaaat attttttatg gtagctggtc ttttagatag tattgcaacc
     7441 ttatctgata tgcttattgc ttctgctata gctaaaaaag tgcaactaat tgcttaaaga
     7501 aacagaatta tccacttcag cacccatata aacaatatta agatttccta aactgctatc
     7561 ccaaacgata ctattaataa tagtagataa taacaatctc ttatcatcta aactggcatt
     7621 atcaacttca tcattaaatc tcttcaaatt actaacaata atatcaatat taatactgtc
     7681 actactaata ttgatgttac tagattccaa agtattaatc ttatccttta aaatactaat
     7741 ctcattattt aggctctcaa tctgggaaac tatataatca gaagcagtac taccagtatt
     7801 ttttgcaagc tctataacta aatttttaat aagtctttcc ttatctaaaa tttgattttt
     7861 aagtaaatta gtctcagatt taaaaatatt aaaaacatta tcagtcttaa tgttattact
     7921 attgaatgca cttataatag actcttcttt acaattctta attttatcta taactaaact
     7981 ttcaactaat ggaccattaa gatttttaca gttgcaacta tcagacccaa gtgttttttt
     8041 agtaccacaa atataataat actttaaatc cttgtcagtt tttttgcctt tataggtaat
     8101 tctcatatta gaaccacatt taccacattt tataagacca gataaaagag cagtttttcc
     8161 agttgatatc cttggagttt tatcacgatt tttatctaat tgattttgta cttcaagcca
     8221 tatttcaggt tctatgactc catggtgttt tgcaattgct gctattgagt tagatgtatt
     8281 tttagcatag gtaaggattc cacattttct atctggagta cctacaacat ccataccact
     8341 atttttaagg taatctaaaa tttcatcatt agcttttaca tatgctggat ttctaagtat
     8401 tctagataaa gaactagggt caaatttacc accacgagga ccccttattc cattattata
     8461 gacatattta tataacttag ataaggacct aagttctaaa tatttattgt atattagctt
     8521 tacaatctcc atttgttcat catttacttg aagcttatac atacttcttt cttttagatt
     8581 ttcatctaag aatgtgattt tttcactgtt gaatccatga ggtgtattgc ctccaagcca
     8641 tctaccacta cgggctaatt catacatatt gtcttttata cgttctgcta ttgtttctct
     8701 ttctaattga gcaaatacac ttgatataaa catcatagct cttcccattg gagttgaagt
     8761 atcaaattgt tctttgatag atataaatga tatgtttagt tcatttaatt tttctattaa
     8821 agtagagaag tcagatacat ttctcgatat cctatctaat ctataacaaa ctaggtaact
     8881 aaatttctta tctttggcat cctgcatcat tttcttaaat tgtggtctat ctatattgct
     8941 accactaaat ccctcatctt catatatcaa aaaatctttt acaccaatag actttgcata
     9001 atcaatacat aattgtattt gattttctat agaatcacct tttccagtaa atcttgattt
     9061 tcttgaataa attgcagctt tcaaaattaa aacctcctat aagttaggat atatatttga
     9121 aactatttat ctacaacctt ttaagtaaac ttattagaat gaatttatta attaggtaaa
     9181 tttatataat tctacttttt ataatatatg actatttatt ttatttcttt actattcaag
     9241 ttc
//
LOCUS       DAGSWI010000151         9241 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.152, whole genome shotgun sequence.
ACCESSION   DAGSWI010000151 DAGSWI010000000
VERSION     DAGSWI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.152"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            442..1647
                     /locus_tag="MJF80_003312"
     CDS             442..1647
                     /locus_tag="MJF80_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBZ0281946.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTNRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1718..1939)
                     /locus_tag="MJF80_003313"
     CDS             complement(1718..1939)
                     /locus_tag="MJF80_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281947.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2080..2679)
                     /gene="recR"
                     /locus_tag="MJF80_003314"
     CDS             complement(2080..2679)
                     /gene="recR"
                     /locus_tag="MJF80_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBZ0281948.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2692..3042)
                     /locus_tag="MJF80_003315"
     CDS             complement(2692..3042)
                     /locus_tag="MJF80_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBZ0281949.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3101..4738)
                     /gene="dnaX"
                     /locus_tag="MJF80_003316"
     CDS             complement(3101..4738)
                     /gene="dnaX"
                     /locus_tag="MJF80_003316"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBZ0281950.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4815..5079)
                     /gene="ffs"
                     /locus_tag="MJF80_003317"
     ncRNA           complement(4815..5079)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="MJF80_003317"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5117..5208)
                     /locus_tag="MJF80_003318"
     tRNA            complement(5117..5208)
                     /locus_tag="MJF80_003318"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5172..5174),aa:Ser,seq:gga)
     gene            complement(5209..5664)
                     /locus_tag="MJF80_003319"
     CDS             complement(5209..5664)
                     /locus_tag="MJF80_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBZ0281951.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5985..7256)
                     /gene="serS"
                     /locus_tag="MJF80_003320"
     CDS             complement(5985..7256)
                     /gene="serS"
                     /locus_tag="MJF80_003320"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBZ0281952.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7382..7587)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7715..8596)
                     /locus_tag="MJF80_003321"
     CDS             complement(7715..8596)
                     /locus_tag="MJF80_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBZ0281953.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8608..9117)
                     /locus_tag="MJF80_003322"
     CDS             complement(8608..9117)
                     /locus_tag="MJF80_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBZ0281954.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNVYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         2960 a         1604 c          975 g         3702 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttctttt tccttttttc
       61 aaggacaagt aatactatat catctttatc tattctagtc aatataattt tttacttttt
      121 ttaatttttt tatttatcat gtattcttta tctttaattt ttaaaactta atgctaagta
      181 aacctgttta tttcataaaa ctaatatata ttctatgctc tattttcctt tttattctat
      241 atttaaattt tttatttaca agaatatttt taatataaca tatttgataa aattaattta
      301 catttctttc acttgtaatt tttgcaatct tgtattagat taatattaaa aataatttat
      361 caataacctt attcacaaag acagttttta tatatcaaaa tataatataa atactatgct
      421 acattttagg aggattttat tatgagcaaa aaagtattaa taatgtctgc ttccactggt
      481 ggtgggcata atagggctgc tctagctatt aaagaagaac ttacttccaa aacacttgat
      541 ggagagccaa ttgaatgtga aataatagat agtttgaaat tagttaataa tacaatggat
      601 aaaataattt caagaggata cgaaaaatct gctatatata ctcccaaagc ctatggaagt
      661 gtatatagat tatctgaaac aaatcttttg tcaaaaaatg aatttaaaga taatctacta
      721 ataacattca tggctaaaaa atttaaaaag cttattagaa gcgaaaaacc agacttgatt
      781 ataggaacgc atccttttcc aatgattgct ctaagtacat tgaaaaaaaa ctttaattta
      841 cataataatg aatcaaatgc ttacacagag catttttata aacattatac aaataccatt
      901 aatgttcctc cgcttatttc tgtattaact gattatacta ctcattctac atggatacaa
      961 aatgaaattg attactatat tgttggtcat gaatatgtaa aagaattatt ggtgtttgat
     1021 ggagttgaac cgagtaaaat tagaacattt ggaatccctg ttgaaaaatc ttttctctct
     1081 cacagagata aagatattgt tctttctgag ttgaatttat ctcctgataa attaactgtt
     1141 ttacttatgg ggggtagctt tggggctgga aatataaaag agacacttga tgaattactg
     1201 gatactaata gagactttca aatacttgta attactggta agaatgaaag cctaaaagaa
     1261 aaaatcgaaa aaaaacttat gtctcgttat catgataaaa atgtatgtgt tcttggatac
     1321 actgataaga tgaatgatat tttagcttct atagatgtat tagtaagcaa acctggtgga
     1381 cttactacaa ctgaggctct tttaaaagat gtacctatga tagttcctta ttatatacca
     1441 ggtcaggaag aagagaatct tgatttctta tctaattgtg gtgcagcact tagaactaca
     1501 aaaaaatata acttgtcaat attactaaaa gttttaatag atgaccctag cagattagaa
     1561 atgctaaaaa gaaatattaa atcaataaga aagtcaaatt cagcccaaaa tatagctaat
     1621 ttaattttaa atataatttc tggttgataa aataaataaa atccctatag tatatatatt
     1681 atctattagg gattttttat tttctaatta tttaatatta aattggttca taaaagtctt
     1741 cttcataatc atactgataa tttttataat tatttctaaa ttcagcattt ttatttcgta
     1801 catttttttt agtttttttc ttattagaaa aacttatatc ctcaaaatca tcatatttag
     1861 gtttatcttt tgatttcatt tttttaactc ttttttcttt cattgcatta tgttcttcta
     1921 agttcataat tggtttcatg ttgcacccct atcaaaattt ttaaatgatt tatacaacaa
     1981 attatacaat acagaagaaa tttattcaat aaatctaaat caaaattaat ttgaatttaa
     2041 ctcaaaaaat tacaaaaatc gaggtattaa cctcgatttt tatatttctc ttctgccttc
     2101 tagtgctttt gatattgtga cctcatctgc atactctaaa tcgcctccaa caggaagacc
     2161 atgagctatc cgagtgactt tgattcccat tggttttact aacctagcaa tatacatggc
     2221 tgtagcttca ccttcaatag taggatttgt tgccattatt atttctttga cttcctgatt
     2281 gccaagtctt tggataagtt cctttatttt taacatatct ggtccaattc catccattgg
     2341 agaaattact ccatttaata catgatactg tcctttaaac tctctcgttc tctccatagc
     2401 tgctacatct cttggatctt ccacaacaca tataacactt gaatctctac ttttatttga
     2461 acacatgcta catgggtctg tatctgttat attacaacaa attgagcaat atcttatttc
     2521 tctttttgca tctataatcg ctttagataa agcttccaca tcattagtat tcatattaat
     2581 aatatgaaaa gccaatcttt gagcagtctt tcttcctact ccaggaagct ttgaaaattc
     2641 ctctataagt ctcgttatgg gacctgtata aacttgcata aataccacct actagaataa
     2701 cccaggtata ttcatacctc cagttacctt actcatttga cttgcttgta tatcatcaat
     2761 atttctcaat gcttgattta ctgcacttaa aactaaatct tgtagcattt ctatatcatc
     2821 tggatcaact acttctggct tgatagttat atctataact tcttttttac cattaacttt
     2881 tacagtaact gctcctccac caactgaagc ttctacttct ttactttcta actcttgttg
     2941 agctttttgc atattttctt gcattttttg agcctgcttt aacaagttgt tcatatttcc
     3001 accaccaggc atcattccgc ctccaaaacc ttttttagcc ataaacacta ttcctcctaa
     3061 acttattttc attttattat tatatcataa tagtatttta ttatttactc tcattttcat
     3121 caatactatc ttttatttct aatatctctt cactcactat gttctttaat atttcctcac
     3181 caatgtcctt tttctcttta acttcaagat ttatatcttt aacttctgat gtcagtacta
     3241 tttttacatt aaaacttcta tttaaaacat ctcttattgt tttttctaaa taatttattg
     3301 tatcttgaga gctcagtcta gtttttgcaa aatcaaattt atcatcaaat ataacataca
     3361 acatattaga atatacatta aaacttttta cttcacttaa gagtgcgtat attggcattt
     3421 ttttatcttt tttaatttgt ttaagtatat tcttccaaga tgactctata agtataacgt
     3481 cctcattttt tacatcttca taaactacat tttcttgttt tacatctatt tctctatcga
     3541 cctcatactc tttttcttta ttattcccaa ttttttcaga cttaaagttc cccagttcaa
     3601 tcattttttc taagttctca actcttttta taagagcttc tttactttca tcaaacattg
     3661 gttgagcaat tttcattatg gttatttcca ctaaaaccct tggatttgaa gatgacttta
     3721 tatcatcctg agttattgat aatatattta aaatccttat taaatcattt atatctatat
     3781 ttttagactg aattttaagt tgttctatag tttcttctgg taatgaaatt atttcatcca
     3841 attctgatga aaccttacat accattaaat ttctaaaatg atcaattaag tcatttataa
     3901 gatttcttat atctttgccc cataagacaa attcatttaa tatctcaaga gactttttag
     3961 tgtcctgctc tacaatacat tgagacaatt caaataattg ttctatattt acgcttccca
     4021 ttaattcaac aacatcttta taatctatct tactttctcc aaaagacata cattggtcta
     4081 atatactaag tgcatctcta agagctcctt gagaatttct agctattaaa ttaagagctt
     4141 tatcatctat atcaattcct tcatcttcac aaatcttttt cattctaagg gtcatatctt
     4201 taactgtaac tcttttaaaa tcgtatcttt ggcatcttga taaaatggtt gcaggtattt
     4261 tgtgaggctc tgtagttgct aatataaaaa ttacatatga tggaggttcc tctaaggtct
     4321 ttaataatgc attaaaagct ccttgagaca acatatgaac ttcatctatt atatatactt
     4381 tatatttagc attggctgga gaatatttca cactttctct aagctctctt atatcatcta
     4441 cactattatt tgaagctgca tctatttcta caacatccat tgtattatct tttaacacag
     4501 attcgcatat ctcacactca ttacacggtt cttcatttat tttattcaaa cagtttactg
     4561 ctcttgaaaa aatctttgca gtagatgttt taccagtacc tcttgttcca caaaacaaat
     4621 aagcatgtcc tatattatca ctatatattt gattctttaa agtcttaata atatgatctt
     4681 gaccaataac atcttcaaat ttttgtggtc tatatgctct atataatgct ttatgcatct
     4741 ttttacctct ctttagcaaa attaataaaa aaatccctca ttatctaagg gattagtctt
     4801 ttatatatta aatgctgtac accatctatc gacttaattc cataggcgtt atcaaaacag
     4861 ttagctcaac ttaggcaccc ccacggcaca caaaagtgat cacttaccgc tgcttccttc
     4921 cggatctgac ggggttcatg agctttcatt gcgcaggacc taagcatcaa caccacttat
     4981 ttagggcggc cctacagaaa ataagccttt agatggaatt caatcctgct atagcggatt
     5041 gcaggttaca gggcaccgct acctccccat ctagtacagc aaaattacaa ccatatttaa
     5101 ttactttcat tgtttctggc ggagagagtg ggatttgaac ccacgataca gttttaccca
     5161 tatactcgct ttccaggcga gcgccttcga ccactcgacc atctctcctt aatctctttt
     5221 tctcaatgcc ttaaaaaatt cttgcaatat gatcgagcat tcttcttgca aaacatcaaa
     5281 agctactttt acattattat tattaaaata atctaattta aactcatgct gtttttctat
     5341 atatgaattt tttacatgtc ttgtccctat tataagcttt tttattctag aattaactat
     5401 tgcgccacta cacattatgc aaggttccat tgtaacatat aaatcacaat cagtcaacct
     5461 ccaaccacct aatttttctg atgcttgttt tatcgctaaa atttctgcat gtgccgtaga
     5521 gtcttttaac gtttctgtaa ggttatgagc tctagctatt atttgattat ctttaactat
     5581 aatagcacct attggtgttt cttttttatt atatgcttta tatgcttctt ttaatgcttc
     5641 tttcatataa aatgaacttt ccataattcc tcccaaaact tataacaaac ttatcttatc
     5701 ataattcatt ttataattca acttaatttt attttgttta tttaattatt actatatata
     5761 gctatatttt tttaaaatta gttctaaaat aatttttaga tattcataca aaagtgtttt
     5821 ataaaaatat ttagtgtttt atgatgaaaa ttctgctgtg taaattaaca taaaaaaact
     5881 atgaaattaa tatttataat aatttcatag tttttttatt tattgtttac tcataatata
     5941 ttctcttcat caaagcatac tctacatata caattaatat aaatttattt tataacatca
     6001 acacccatat aaggtcttaa ttcttctgga actactacag aaccatctgc ctgctgataa
     6061 ttttctaata tagcagccaa acatcttcct attgctaatc cagaaccatt taatgtatga
     6121 acatattctg cttttgattt tttatctctt ttaaatctta ttcctgctct tcttgcttgg
     6181 aaatcttcaa aatttgaaca agaagatatt tctacatatc tattgtaact tggcatccaa
     6241 acttctaaat catatttaaa tgcagctgta aatcccaaat ctcctgtaca tatttttaca
     6301 actctatatg gaagacctaa catttgtaac atagtttcag catcatgagt taatgattct
     6361 aattcattat aagaatcttc tggtttaaca aatttaacta attcaacttt attaaattga
     6421 tgttgtctaa ctaaacctct tgtatctctt cctgctgaac ctgcttcaga tctaaaacaa
     6481 ggagtatatg cacagtattt aattggaagc tctgcaacat ctaatatttc attagcatgt
     6541 atattagtaa caggaacttc agctgttggt attaagaaat actctaatcc ttctatttta
     6601 aacatatctt cttcaaattt aggaagttga ccagtcccta taaaactatt tctattggcc
     6661 ataaatggag gtaagacttc cgtatagcca tgcttagctg tatgagtatt taaaaagaaa
     6721 ttcattaaag atctttctaa tctagctcct aatcctctat ataaagtaaa ccttgaccca
     6781 gttattttcc ctgctctttc aaaatctaaa atacctagac ctgtacctat atcccaatgc
     6841 gctttatgct caaaatcaaa tttagtaggt tctccccaag ttcttatttg aacattgtct
     6901 tcatctgttt caccttgtgg tacctctgga tgaggaacat ttggtattct cattaatgta
     6961 tattccattt ttgcttctac ttcttttact ttctcatcaa taccttttat cttatctgat
     7021 aactctttca atcttgcctt ttcttctgtt acatcttttc cttcttttat taattgaggt
     7081 atttttttag aatttacatt taactcattt ttcataactt caacttcttg aagtatttct
     7141 cttcttttat tatctaattc cactacagca tcaaggtcaa attctctttc acctcttctt
     7201 tccatagctt ttttgatatc atctagattt tctcttattc ttttaatatc taacatatta
     7261 attcctccta ataattttga taatataagt tataattcca acttaattta caaacattat
     7321 acttgtaaac tcttagttta aacaaatttt aaaaataaaa aaagacctcc gtttacccta
     7381 taaaagggac gaaaatcttt tatccgcgtt gccaccctaa ttgactatac taaaatgtat
     7441 aatcctctca gacctataac gtaggtaaac gtctagcttt aactagaagc tctagagtag
     7501 attcaaaaac cattgtaatg agttttcacc atccactcat tctctgaata caatctgttt
     7561 tttactattt tccttcacag ctttttaact aattgtttta tttgataatc attatattat
     7621 atatattaac atctttcaat actattattc aaattttttt agaattttct ttaatttttt
     7681 atattaaatt cattttttct atttaattaa gaccttatct tttatccatt gtatttagat
     7741 tacctgtatt attaaataat aatgaagaag gaacaaatgg aacttttata tcattctcat
     7801 caaatttatt ttttaccttt tccattacat caaaatatac atcccagtaa tcatcagttt
     7861 ttacccaagg tctagcaact atttgcatgg tgttatttcc tatagagttt aaacctatta
     7921 taggctttgg atcatttaat attctttttt cgtttgtaaa aatttcattt aatatagact
     7981 taactacatc tatatcacta tcatatgaaa tattatatga aaaatctatt cttcttttgt
     8041 tttgatgtgt gtaattaatt atattattac ttgtcagttg aaaattaggt ataattatag
     8101 ttttattatc tggcgtctta agaacagtgc taaatatttg tatgtcagaa actgtacctt
     8161 ctacacctga accttgaata taatcaccta tattaaatgg cttaaaaaat aaaataatca
     8221 taccagagcc taaatttgat aatatttctt taaaagctaa acctatacta aatccagcag
     8281 ctcctaacac agctacaact gatgtagttt gtatccctat aatacctaag cttatcaatg
     8341 atataataga taacatagat atatatattc catatacaat aaaattattt acacctttac
     8401 tcattccata tttgattaga aactttgaaa ccatccttct tactatttta gatatataaa
     8461 tacctatcac aaatacaatt attgcatata taatatttgg tatattatca ctaaaattat
     8521 cgagcatatt ttctaaagtt ctcttagtta tattgctatt tgttgaggca gtcttagcca
     8581 atgataaaaa attcattcaa ctcctcctta tgataatatt ccgttgtcct ttaactcttt
     8641 tactatagct tctatagttt cttcttttat tgcctcaatt gtatgaatat ggctacattc
     8701 tgtcagaacc gataattgtt taaattcatt tgtctcaact ttcttcataa agttgtctat
     8761 atccatttta gaataaacat taagttctgc agttatctga ccatatgttg gatgatttac
     8821 aatgacatct tttataatac cacctaaatc tacaattatc tttaattcat cataaatttc
     8881 tttagaatcc ttatgatttt tacactttat ttgttttgta aaatacggtt gattatatat
     8941 tatatatcct tgaggtgtag ctattatttc aaatcctcta gctcttataa gggctatatc
     9001 ttgaactatt acttgcctac ttacatttaa tagttcagct agtgttccac cttttacagg
     9061 atgcttagat tcttttaaaa tatctattaa tttacttctt ctttcattag aattcatatt
     9121 acaccttctt tccaaataaa aactttacct atactatata attttatatt ttatcttatt
     9181 ataaatcaat attgacttta tattctattt aatcttaact tttataataa ataaaaaaag
     9241 a
//
LOCUS       DAGSWI010000152         9235 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.153, whole genome shotgun sequence.
ACCESSION   DAGSWI010000152 DAGSWI010000000
VERSION     DAGSWI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.153"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(245..853)
                     /locus_tag="MJF80_003323"
     CDS             complement(245..853)
                     /locus_tag="MJF80_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBZ0281955.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(962..3700)
                     /locus_tag="MJF80_003324"
     CDS             complement(962..3700)
                     /locus_tag="MJF80_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBZ0281956.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRTFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3848..4639)
                     /locus_tag="MJF80_003325"
     CDS             complement(3848..4639)
                     /locus_tag="MJF80_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBZ0281957.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4675..5331)
                     /locus_tag="MJF80_003326"
     CDS             complement(4675..5331)
                     /locus_tag="MJF80_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0281958.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5324..6289)
                     /locus_tag="MJF80_003327"
     CDS             complement(5324..6289)
                     /locus_tag="MJF80_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBZ0281959.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6395..6501)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6791..7735)
                     /locus_tag="MJF80_003328"
     CDS             complement(6791..7735)
                     /locus_tag="MJF80_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBZ0281960.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNVVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKCCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            7846..8313
                     /locus_tag="MJF80_003329"
     CDS             7846..8313
                     /locus_tag="MJF80_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281961.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8483..9082)
                     /locus_tag="MJF80_003330"
     CDS             complement(8483..9082)
                     /locus_tag="MJF80_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281962.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         3029 a         1508 c          877 g         3821 t
ORIGIN      
        1 ataaaataaa ctaattatct atacaaattt tataattttt aataaaatac acaaaaaaat
       61 taatttttat agctcatttc gccaaaatat atctttcttt atatcattaa aataattata
      121 aatttattaa atatgtttgt aattctataa agttattttt atcaaactaa aaattctata
      181 aaaaaataag atacatacct ataaaatacc ttcctataga tacatatctt acctatataa
      241 aaacttattc tatataaaaa cgccataaaa aatcttttgc ttcttctgca taatctatat
      301 ttatacgttt actagttttt atatctaact catctttact aaatactttt tttcctttat
      361 catcataata aaaatcactt atatataatt cttcacccat tatactctta ctatttagac
      421 ttctatctat ttttaaagct ttgcaaagtt ttcctggtcc gttagtaata ttttttactt
      481 gatatttact caactcagtg taagctttgt tgtatctatt catagatatc tcatctaaag
      541 aagttatcgg ctctacccct cttattaata cacactctgg cacattttct atatttgcag
      601 ataaattcaa gcaattatac ataccataaa ttaaataaac atatatatgt ccaccatcta
      661 aatataatgg cttagttcta tctgtctttt tattgccata tacatgtgct cctttatcat
      721 ttacacccat gtatgcttct gtctctatga ttttggtaac aattacttta ttttcatatt
      781 tccttattag atatttacct aatattgatt tggctaaatc aattccattt tttctaaaaa
      841 aatccttttc caaaatttca cctcataaaa tttatttaaa acgcatcaaa tacaactaaa
      901 gtaataaata aaattaactc ttattaatgt gatatatata aacaataaat ttagacattt
      961 attatttata tatatcaaaa aattctatct caaacttttc aatattatca ttgtaatttg
     1021 aattatcata tacttcttca acaacctctt taattgggaa tataactttt acattagttc
     1081 cttctcctac cttactatct atgtaaatct tcccattttg aatttcaatc aatgattttg
     1141 taaggcataa acctatacca cttccaaatt catttgatat accactgttt tcaatttgtt
     1201 taaatcgatt aaatatatca tttaattttt ctttgtctat gcccacccca gtatctttta
     1261 taattatctc tactgtttcg tcttttttat atatacctac aaggatttct cctccttcta
     1321 aagtaaattt tatagcattt gataatatat ttagtattat tctttctatc gattctgggt
     1381 caagccctac cataacagtt tcttcatctg tatcaaaaat taaatctatt cctttagact
     1441 tagcataact actaatagat gtaactattc tttctgttat gtcaataata tcaaaattaa
     1501 ctgctttaat cttatatatg tctgacttaa cttgagaaat atttattata ttatctatta
     1561 atctcaataa tctaaacata ttttgtttag ttatttttat atgttctttt agtatatcct
     1621 gaaaatcaat tatttttcct tttttataaa ggttttcaat aagttgaact gaactatata
     1681 tattatttaa aggtgtctta agttcatgag atatattagc ataaaactcc atcttcattt
     1741 ggtcatactg tctttgtttt attaatgctt ttttaagtct ttcagactct ctttgcacac
     1801 ttaaatcttt aataattctc actctacttc ttttctgtcc aaagtataat gttcttgata
     1861 tcatttttac ttctttaatg tgattatttt catttactat atggatactc ttctctcttt
     1921 ctcctatatt taaattgttt atgtattttt ttactaatat atttctatat ttttgtgttg
     1981 taactcctaa tacatcacta gaaattccga acaactctct aaaggttcta tttgtatata
     2041 gtactcttga tgtacttaaa tcctcaagaa aaatagcctc tggtataaca tcaagtaatt
     2101 tttcatatat tattttttgt tcatttactg ttctaagaat atttatctct ttagttatat
     2161 ctctaaatgt aagcaattta aagagtttat tatctgacca atagtctcca actctaactt
     2221 cttcaacatg ttcttcacct tctgagtcag acatcacaaa attaactata cagtttgctg
     2281 tgtcattgta acttactatt tcagctttct tttcttgtgt tagatttaat cttttaaatt
     2341 ccctattgtc aaagaaatct atgttaaaag tttcaaaata ttgttctaat gtcatatcga
     2401 ctaaatcact tgaaacacat ttaaaactct tttcatattt tttatttata cttattacct
     2461 tattcttctt tatgccaccc tcagcacttc ttacaagtat aggagatgga atatttagta
     2521 taatgttttc atagtcttta tttatatttc ttatttcttc atctattttg atttcatttg
     2581 taatatcatt taaacttatt aagtaattgc tttcatcttt attattactt aaacttaaca
     2641 aataaatttt atcatttaac tctaccagtt tatcaattat acttatatcg ttatcaaatt
     2701 tcttcaattt ttcagcaact ttttcatctt cctttataaa agttaatatt ttataataat
     2761 tattcatatc ctttgaaaat tcatctttaa atttattatt tgtgtaactt atattaaaat
     2821 catagtctgt tactatccat acatttggta ttgtatccag agcttgtcta agtaattctt
     2881 ttctctggtc tatgtagttt tttaatgttc tttgtataat catatccttt tctattgcta
     2941 aatttatctt tcctaattgc tctgactttt tacttaattt tttattgatt gaatcaagtt
     3001 cataatatgg atacactact ctattattaa gactgtatac atatacataa taagtatggt
     3061 tacataatag taaatatatt actcctatta atctatctga atctactata ttaatcttag
     3121 aaattgctac aaaacatata atagttgtaa aaaaaacaaa atatgttaaa tcttttatta
     3181 acttatagtg ctcaatgtta ctatcaagtt tattcaacaa atatagtaaa ttaaacatag
     3241 atatacaaat attaattaat ataataatca tattccagtt aatatcacta aaaatatttc
     3301 ttataaagta atctaatatc acaaaagcac tataaaaaat tagtattggg cttattctta
     3361 gtttttttac tttagaaatg gttaagtata taaaagtaga catattaatt atgcttaagt
     3421 taagcaatac aatttggaat ttttgaattt ttaaatttac tactcctaaa atacttacaa
     3481 aaactaatac aagacaccct actttcaata tatttataaa ttcatctgtt ttaaagctac
     3541 ttatataaac tatagaatat ccaaatatcc aaagtaaaat tattctatac atatttatag
     3601 aaactgtata caaatttata tttaaaagtg caataaccaa aaataaccca ttagctagta
     3661 tatagacctt attcttactt aaagttcctt tagattccat attccccacc acttcttcat
     3721 ttttatatag ttttacctat aaatatgtta ccacaattct tccaaaaaat ttgtcacttt
     3781 ctagttataa aaaaaaatga gtaaatattt ttaattttac tcattttgta gattttgtat
     3841 attcagttta aaaagctgga actatacttc ctttatattc atcttgtata aattttttaa
     3901 cttctttact attcatagcc tctgataaag ccttaatctt atcactatct ttattatttt
     3961 ctctacaagc tattatattt acatatggag aatcacttga ttctattact attgcatcct
     4021 tagtaggatt taagttagct gttaaagcat aattagaatt tattactgct ccgtcaacat
     4081 cttttaatac tgttggtaat tgctctgcat tcatttcttt tattactata tttttaggat
     4141 ttttagttat atctttttta gttataagct ctccatcttt tacttctatc aatttatttt
     4201 ttgctaataa tttaagtgct ctagctccat tagttgcatc atttggcaca gctataacag
     4261 ctccatcttt taattcatct aatgacttaa ctttttctga atagaatccc attggctcta
     4321 tatgaacttt agatgtataa gtaagtttat atcctttctc cttcacagtt tcctctaaaa
     4381 atggaatatg ttgaaagaag ttagcatcca aagacccttc atctaaagct gtatttggaa
     4441 gtacataatc atcaaatatt tttacttcta aatcatatcc tttttctttt aacaaaggtt
     4501 ttgcaacttc caatatcttt gcatgtggat ttgatgaagc tcctactagt atttttttat
     4561 cctctttgtt tgaacaacct actgctgata ttgctattgc tgacaccaat gctactgata
     4621 ataatttctt taatttcatt tttactcccc ctctttaaaa tgtttataat ttatttattt
     4681 tagtttctta taagccagat ttcctactcc ctggattatc tgaactaata ttataagtgc
     4741 tattacagta tataccatta ctgctgtatc aaatctttga tatccataaa taagtgctat
     4801 attacctaat cctccagctc ctactgcacc tgccatagct gtataaccaa gtatagatat
     4861 tattgataat gtaattccag atactattga aggcatagct tcctttatca taactttaaa
     4921 tactatttgt ctttttgtag ctccaaaaga ttttgctgct tctatcaatc ctttatcaac
     4981 ttcatttaat gagctttcta taattctagc gataaacggt gctgccccaa tagttattgg
     5041 cactattgca gcagtttccc ctatacttgt tccaacgatt aatctagtta ttggaatcat
     5101 tgcaactata agtatcataa atgggaaact tctaaatata tttactataa atcctaatgc
     5161 actatttatt gtgctatttg gttttaatcc atctggtttt gtaactacta atatgattgc
     5221 taatataaat cctaatattg ttgccactat agttggtact ataaccatat acaaagtttg
     5281 taacaatgca tttggaaata gtgttgttaa aaagtcaatt aaactattca tttatcattt
     5341 cctcccatct tatgccctta ctagttagat attgttttac ttgctctcca cttttatctg
     5401 aaatgtttat aataagtgaa cctaaaatat catctttaaa ttgttctaat ttcccaaata
     5461 ttatagacac atctatattt agttctcttg ccatagtcgt aataatagct tcattagaaa
     5521 tatctttagg gaatagtatc tttatatttg ttccttttgg caatattata ctttcttcac
     5581 ctattaattt tctaagtcct ttagtatttc ttaagaatat ttcttctgta tctcctacct
     5641 ctaatacttc tccattttcc attattgcta ctcttccaca aatctgcttt ataacttcca
     5701 tttgatgagt tacaactatt attgttattc ccaatttttt atttatatct tcaagcaatg
     5761 aaagaattga ctttgtagta tttgggtcta atgctgaagt tgcttcatca caaagaagta
     5821 cttgtggatt tagtgctaaa gctctagcaa ttgcaaccct ttgtttttgt ccgccactta
     5881 gatttctggg tttatcattt ttcttttcag atattccaac tacttctaat aactctaaaa
     5941 ctcttttttt aatttctgcc tttgagtatc caaaacattc taatggcaat gctacattat
     6001 caaatacagt ttttctttca agcaatgaaa agtgttgaaa aatcattcct aactcttttc
     6061 ttaaatctct catttgcttt tcattcaatg actttacttc tttatcagaa acaagtacac
     6121 tgccttcttg gtattcttct aatccgttta tacatctcag tagtgttgat ttccctgcac
     6181 cactatgacc tattattcca aaaatttctc cagattctat ttcaatactt acatctttaa
     6241 gcacttgaat ctttccataa tacttattaa cattttttat gctaatcata aaatttcctc
     6301 ccatatattc tgctcagttt ttatgtttat cctctcttat gtattacaac aaaataaaag
     6361 cccgagtata aactcaggct tagaaagcta tttatactca tctatcaact atattagttg
     6421 caggatttag caccaatgta tataaaatat actggttgcc gggtttcaaa gggccagtcc
     6481 ctccaccact cttgataaga cgttatttaa tttaattcta ttatcatttg ttgctatgtt
     6541 atatatgata tattcattaa tagtatttgt caataaattt atttaaattt tttatttttt
     6601 attatcgtta attaactatt tttaattagt tttctagctc ctaacacttt atattttaat
     6661 gtctatataa ctatagaaca tcattataag aaataaatta aataacatca atctatctaa
     6721 atcatctgta aagtaaatta agaaaattaa cattttatcc aaactctatc ctactattaa
     6781 ataaaataat ttaaatacaa tgatgattat atagctctag tactttagac tttatcaaat
     6841 gtttaagttc tataggttct attattgtag catttttccc aaaagttaaa aaatattcag
     6901 ccacccagtt aagtgtagat tttggtatat acatatctat gtatccacct ttagacaaga
     6961 ttttcaatcc tcttgccaac aaaaattctg tttcacaaca ttttactccc tcatctgtca
     7021 aattaatttt aatatgataa tctgtaccta cttccatatt cttaagataa tcatgtatag
     7081 aagataaaac cttatatttc ttacttgata agttttctat actcaaaatc ttcacaattc
     7141 tgtctacacg aaattcctta atagattctg attttataca gtaagctgga caataccaaa
     7201 gtccattcat ggcatacaaa ccaataggaa caatggtacg tacactattt ttttgctttg
     7261 atgaatattc aattttaatt gtagattcat tcattactac attaaacaat tctttcaata
     7321 atggtgttga acagtgccta tctggtgtcc aaaaaacaaa tttacgcctt atattctcaa
     7381 tattatattg aatatcattt ggtatacaat ttaaaaattt ttttaaaaca gaaatagttt
     7441 cttgctcaaa tggcaaatca ttataatatt ctagtgactg atatgcaaag aatatagatt
     7501 ttacctcact ttctgaaaat gcaatcggtg gaagtatacg ctctttaagt acatgatatc
     7561 caccagccgc tccaactttt gaatataatg gaaatccaat actctccagc acctgtaagt
     7621 ctcttagtat agtacgtttt gatacagaaa attcttctgc taattctcct gctgtaaaac
     7681 ttctttttgc atttattgtt atcatcagtt caattaaacg ctcagctctt gacatattta
     7741 tactcctaat ttaaatatag tgacaataat tgtcactatt atatgataag atattcttac
     7801 atttaaagca ataataatag taaagaaaga agggactgat actttatgga aaataatact
     7861 attaaggctg atgatattat caattattgt ctatcaaact tagatgatgt tttattaatg
     7921 aatagttggg gggaacgagc aatttactac aatcctaatg gtattttaaa gcgaggagta
     7981 tatgttctta ccattaaaga aaaagacagt aatcatgata aaggttcgtt ggttagtcgt
     8041 ccaaatgtat accgtgtgaa tataggatta aaaaaagaaa cttttattga aatgtttgga
     8101 tatattccaa agcgtccagg tgtaggtcaa atagttgata tggattttga ttttacaaaa
     8161 ttggacacaa tcatgccaca tcctatctac tcatggatgg gatggatatg cgtcctaagc
     8221 cctactgaaa agacatttga gaactttaaa actttaatag gagaatctta caattttgca
     8281 aaacaaaaat ttaaaaagag aaaaaacaag taaaaacata tgaaaataat ccctaaataa
     8341 attataataa aaatattaag gttacaagat aaaaattaat atcttaataa catgcaaaaa
     8401 agcgaagcca aaaaatgtat tatttataag gcttcgctat atctcaaata atttatttca
     8461 aatttcaaaa ctatttattc tattatttct tattatttcc tgttattcct aatctatcat
     8521 tcatatcttt tatcattttt ctaaaaccac tacctatatc atttccaaca tcttcagttc
     8581 catgaactaa ttttataaag tcattttgac cttttttcat atcttcatca gataccttat
     8641 ctaatttttc tattctctta tctatgtctt ttactaatct ttccccttca gcgattaggt
     8701 catcattcat tttctttaac tctgtatctt ctgttttacc atctttttta agagatgtta
     8761 caaatgattg tattttatct ctactatctt tatatgcagc ctttacatca cttagatatt
     8821 tttcatttcc tggatattct ttatctttaa actgattatt tatactttct ggagtatcat
     8881 aaatagaata tctatttaga cctgctatat aatcattata gcttgttgaa taataattca
     8941 tatatccttc attattattt acagattcat taggattttt tcctgtattt tcacttggtt
     9001 tttcattatt tttagtacat gcagttatac ctatagctaa tactagtgct aatcctacaa
     9061 ataaagttct ttttaatttc atttttatta ccctcctaga tgttttatac tataaaaaat
     9121 tttaataatt ttctttctta atttagtatt tctatatttt tatattttat ttactaaaat
     9181 taaacaagtt ataatttatt tttttggaaa tcataatgaa taaataatta aataa
//
LOCUS       DAGSWI010000153         9224 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.154, whole genome shotgun sequence.
ACCESSION   DAGSWI010000153 DAGSWI010000000
VERSION     DAGSWI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.154"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            192..821
                     /locus_tag="MJF80_003331"
     CDS             192..821
                     /locus_tag="MJF80_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0281963.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(935..2449)
                     /gene="glpK"
                     /locus_tag="MJF80_003332"
     CDS             complement(935..2449)
                     /gene="glpK"
                     /locus_tag="MJF80_003332"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBZ0281964.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVDDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            2693..3235
                     /locus_tag="MJF80_003333"
     CDS             2693..3235
                     /locus_tag="MJF80_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0281965.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3249..5741
                     /locus_tag="MJF80_003334"
     CDS             3249..5741
                     /locus_tag="MJF80_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0281966.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            6162..6560
                     /locus_tag="MJF80_003335"
     CDS             6162..6560
                     /locus_tag="MJF80_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0281967.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(6777..6986)
                     /locus_tag="MJF80_003336"
     CDS             complement(6777..6986)
                     /locus_tag="MJF80_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281968.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(7018..7227)
                     /locus_tag="MJF80_003337"
     CDS             complement(7018..7227)
                     /locus_tag="MJF80_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281969.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            7526..7945
                     /locus_tag="MJF80_003338"
     CDS             7526..7945
                     /locus_tag="MJF80_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281970.1"
                     /translation="MDTLGERIVYLRKSKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPFNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            8173..8628
                     /locus_tag="MJF80_003339"
     CDS             8173..8628
                     /locus_tag="MJF80_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281971.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(8836..9096)
                     /locus_tag="MJF80_003340"
     CDS             complement(8836..9096)
                     /locus_tag="MJF80_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281972.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
BASE COUNT         3409 a         1316 c         1187 g         3312 t
ORIGIN      
        1 atttttataa ttaattcttt atatagtttt aagtataata aaatcatgta tactaaatgc
       61 atgttaaaat attattttaa aacatgcaca atatttgtac atattgtaga catataaacc
      121 tataaattat acaatagtat tgttaagatt atatattttt ttaacataaa ttttgtattg
      181 ggggaaaaac aatggataaa aactatacga tgccaatata ccaaaaaata gcgttggata
      241 ttgcaaataa aatatacacg ggtgaaatac aagaggattc agtactattt ggacgctctg
      301 ttttggctgg taaatacaat gtatctccag aaacgataag aagagctgtc aaaattttag
      361 aggacattgg cgttgttaaa agcataaagg gtaaaggtgt tattgtacta tctccagaca
      421 aagcttcttc ttttataaaa aaatatagag atattacaaa tatatcatca tataaatcaa
      481 cactttataa tttaatagat actaaatcta atttagaaaa tgaaatatta gatactataa
      541 ataaaatttt agactattct aacagacttg aaataatcaa tcctttagtg cccgttcagt
      601 tcaccataaa ttctaattgc aaatatattg gtcaaacagc tgcacaaact aaattttggc
      661 aaaatacagg tgctacaata gttgcaatta aaagaggtga agaacttata atatctcctg
      721 gaccatatat tgaattttta gaaggagaca ttctcttagt agttggtgac caacacattt
      781 acaattctat accaatgttt ttatatgaaa acgaaaaata aattatataa tttagtatga
      841 ttatataaaa atgcaaaatt aacaacaaaa aaagagcttc atattttaca taaagctctt
      901 tttaaattca aataaattta attttgatta atttttaaat atccaattca tcttcttttt
      961 cccagcttaa agctctcgat acagcttttt tccatccttt atataattta catctcttct
     1021 cttcactcat atttggatta aactcttttt ctactgacca ctttgaagta atttcatttc
     1081 ttccaatata aaatccaaca gacaaccctg ctaaataagc agctcctaaa gctgtagttt
     1141 ctactatttt gggtctatct atatctacat ttaatatatc tgattgaaat tgcattaaaa
     1201 aattattatt acttgctcca ccatcaactt ttaatgattt tagttttaat tttgaatcgt
     1261 tttgcattgc ttctaaaaca tcctttgtct gataagcaat tgattccaat gtggctctta
     1321 ctatatgttc tttttttgct cctcttgtaa gtcctaaaat tgaaccccta gcatacatat
     1381 cccaatatgg agctccaaga cctgtaaatg ctggcactac atatacacca ttagtatcat
     1441 ctaccctatt tgcatacatt tcactatctt ttgcagattc aattatcttt aattcatctc
     1501 taagccattg aacagatgct cctccaataa atatacttcc ttctaatgca tatgttactt
     1561 ttccatcaac tccccaagca attgttgtaa gtaacccatg cttagattta actatgttac
     1621 tgcctgtatt cataagcaag aaacaacccg ttccataagt attttttgca gtaccttctt
     1681 caacacaatt ttgaccaaat aatgctgctt gttggtctcc agcacatccc gcaataggta
     1741 ttttagctcc tgataacatg cgctcatctg tatgaccata aacatagcta gaaggtttta
     1801 ctaatggcaa catgcttatt ggtatgtcca attctcgtaa tatatcctca tcccattcta
     1861 aatcatttat attgtacatc attgttctag atgcatttga gtaatctgtt acatgtactt
     1921 ttccacgagt taaattccat attaaccatg tatctatagt tccaaataat aactctcctt
     1981 gttctgcttt ttccctagct ccttctacat tatctaatat ccattttact tttgttgctg
     2041 aaaagtatgc atctattaaa agacctgttt tttcttttat tgattcttcc aatcctttat
     2101 ttttaagttc atcacatatt tctgacgttc tcctacactg ccatactatt gcgttataaa
     2161 taggtttacc agtataccta ctccaaacaa ccgtagtctc tctttgatta gttatcccta
     2221 ttgaagcaat ttcttctggt cttatacctg ctgtttctat aacttctctc attaccccac
     2281 tttgtgaccc ccatatttcc atagggtcat gctctaccca tccagctttt ggataaattt
     2341 gattgaactc tttttgtgca attttcacta tttcgccttt tttattaaat agtatagctc
     2401 ttgaactggt tgttccttgg tctaatgcca ttacatactt cttttccatc accatattct
     2461 ccttatcata taatatttaa tttattaaaa agaaaaattt aatatcacat atttcaataa
     2521 ataatcatac ttaagtacaa atttaatata atagatattt agtaagaaga cacttatttt
     2581 ctatcatatc tatataataa tattatacat tatagcttac atattgatta ataaatttta
     2641 aaggagtata ataaacagtg ttcataataa ttttaataaa agaggtggta atatgccaaa
     2701 gattttagaa aatgtaaaag aagatatatt aaaagtgtct cgggatatga ttttagaaga
     2761 agattactcg aatattagca taaggaaaat agctcaaagg tgtggtataa gtgcaggtac
     2821 agtgtataat tactttaatt caaaacaaga aataatagaa tatatcacaa aatctgaatg
     2881 ggatttattg ataaggcgaa ttgaatactc caataaaaac actgaagatt acataaaaaa
     2941 actaaacatt atttttactg aaataagaaa ttttataaac aaagttcata atatccaata
     3001 caatgacttt ttaaacactt ttgaaacaga gagatttttt gaaatgaaaa aacacaaaga
     3061 cgactttcat gaacagcttt caaataaagt atatgaagct ttagaaaaag aaagtttttt
     3121 taataatgac aaattagtat gcaatatcat aataaaaatg tttttttcat attcaacata
     3181 tcttcatata gaatttgaag atttaaaacc ctatattgaa aaattaatac attaaggtgg
     3241 tgtttacctt gaatcataat actagtatta agaacaagga cagtaatcca aaaatatttt
     3301 tagtacttac actttatcta ttaggtatat ttatgggagc tattgatact ggaatagtat
     3361 caccagcaag aacgattata caaaatagcc ttggagtaaa tgaaaaaacg ggaatttgga
     3421 tgattaccat atacacatta acatacgctt ctgtaattcc tatctctgga aaattagcag
     3481 ataaaatagg aagaaaatat gtgtatctag ttagtatatt tctatttggt agtggttcat
     3541 taatatgtgg attatcatca ctattttcca atttctatat tttattaatt ggtcgtgtta
     3601 ttcaagcaat tggtggtggt ggaatcatgc caattgctac agcagaattt ggaactacct
     3661 tcccagaaaa taaaagaggc atggctttag gtttagttgg ggcaacatat ggtattgcca
     3721 atattttagg ttcaagcatt ggtagcacaa ttctaagtat ctttggaact caaaattgga
     3781 agtggctatt ttttgtaaat cttccaattt gtttaatcat acttattggt ggagttttct
     3841 gtattaaaaa caataagtct gaatctaaag aaaaaataga taagcttggt acattgatgt
     3901 tgatatgtat aatcgtatca ctattatatg gtcttatgaa tatagacttt tttaatttta
     3961 aaaattctat acaagatata tcagtttatc catacttatt gacatttatt gtacttatac
     4021 ctattttcat atttattgaa aatagagcta aagaccctat tttaagcttt gaatattttt
     4081 taaatccgag aatattgata gtacttattt tatctttaat tgttggtatt ggaatgatgg
     4141 gtatggtatt cgttccacag tatgctgaaa atgcattaaa gataaatgct ggttctgggg
     4201 gatattttgt agctatattg ggattatttg caggtattgc tgctccttta agcggaagac
     4261 ttattgacaa gtttggggca aaaaaaatac tattactagg attttctgta tctatgatag
     4321 gctctcttta cctagtttta attgctctaa aaaccaatac tgtatttagt gtatgtgtaa
     4381 gtttaatatt tatgggatta ggaatgggat ttactatggg aactcctcta aactatatga
     4441 tgttatcaaa tacaagatta gaagaatcta attcagcact tgcaacatta tccttgatac
     4501 gctcaattgg tacttcaatt tctccagcta taatgattgg atttatagca catgcaggat
     4561 tatctgtgca agataatatt atggacttag ttggaaagcc ctcaactcct aaaatagttc
     4621 aacttgaaga acttgaaaac atgatagatg acttaaaatc agaccctgaa atggcaaaaa
     4681 atttaaaaaa tgtctctatc ccaaatatga atgatacttc aaatataaat atggacatga
     4741 caggtggcaa acttccaaaa tccttactta ataaagttca gtcagcagat acaaccaaca
     4801 ttaccaatat aacaaaagaa atatcaacaa caatgtttga tgaaaaagtt ccatctataa
     4861 ttactaaaat acagaaaaat gtacaaaaag gaatagatgg gacacaaagt ggaataaacg
     4921 gtattgaaga tggtgaaaat aaactaaata gtggtattaa aggagttcaa actggtattg
     4981 acaatatggt aaaagctaga gctggtatta atcaaggaat agatggtgta aaaaaaggta
     5041 ttgctggtgt tgataaaggt ataaaaggta tggaacaagg tcttgaaaaa caagatgagg
     5101 ccatcaagga acttacaact gcttataata aaatacctac tacagaaaaa actcctactg
     5161 ataacgaaaa taactcaaat gaacaaaacc agagtaatac cagtaattct gaatcagatg
     5221 acactaagaa tgattctaca gaaaataaga ataacaattc cagcgagaat aaaccaaatg
     5281 taaatgaaca aaaagaaagt ttaaatgctc aaatacagca actaaaaaag acaagagatg
     5341 acttaaatac aaagttacaa aaaaataaaa ctcaaaagaa cgaattaagt aaaaaactaa
     5401 gatcaatgga atctcaaaag aaagagctac aaagcaagtt aaataattct ataagccaaa
     5461 agaaagacat ggaaaaaagc cttgatacaa tgcaacagca aaaaaaatca ctccaagcag
     5521 ttttagcaaa gacacaagag gtaaaaactg aaataccaaa ggcttttgat aaatcaaaat
     5581 tggattatat aaattccata gaaaataata gaacaaaaat agaaaacaca ctacaatcta
     5641 ctctaaactc aggatttaaa cagatgtata taacagtatt ctgtgtaaat ctactagcat
     5701 ttataatatt attgttctat aaagaaaata aaactagata aaagagtgtt atagtattat
     5761 attgtaaatt tatcttattc ttaaatattt tattctaaag aataaattca taaaattttt
     5821 gtaattgatt aatataaatt acaatataat actatttttc tcttaatctt tttctctttt
     5881 acagacaatc aaaaagcaat cttagtttat cgaaataaat ttatacaatc tattcataca
     5941 cccctttaca tttttataac aattaaacct attgttatat atacaaatca aaataaacca
     6001 tatattttca actataatat gttaaaatca tattatgagc atattaattt aagcaaaatg
     6061 tcatttagac tttattttag tagttttata ttataaattt atatattttt aattttaaat
     6121 gtcatttaga cattactaat aaaaagaaag gagtgtattt tgtggatact ttaggaaaaa
     6181 gaattgctta tttgagaaac tccaaaaaac ttactcagcg caaacttatg gatattttaa
     6241 aatttgaaaa cttaggcaag tatgaaacag gagatagaaa acctaattgt gatatactaa
     6301 tgtcaatagc tgattatttt aatgttacga ctgactggct tctctatggt aaagagaagg
     6361 taaatgtcaa tagtagtgta aaagaagata aagaagatta cttacatgtt actaatgatg
     6421 aaatgacgat attaaatcta tacagacaac taaacgaacg tgataaaata aaaatagaag
     6481 gaatacttga acttaagata tctgaatata aagacttaaa aaagcattcc tctaataata
     6541 atgaagataa aatggtttaa acatacataa tttgatacga ttttacatca tctattaacc
     6601 ttcttttttt gtagtatatt actaaaatat ggatattgga ttatttaaaa acaagtatag
     6661 tctaatttca agcacattta taatatttag attaccttat ccatatttta gtttaagtac
     6721 tttgaaaaat ataatacatc tctatgtata acttatattc ctaagaaatg cattactcat
     6781 gtttttaatt cttccaacat ctttctatac acaattgtac atccttcata attcccctct
     6841 ttaagaagga ttgaacatgt actagaatat ttagttaaag tatttaatct ttccatattt
     6901 aagcttttta tattactgag tgctgtaata aaatcatcaa ttgctctaca acactctata
     6961 accccatctg aatccaattt acacacaaga atattcacct actttatata ctatttatta
     7021 atcatcttct gaatattcta tattaagtaa ttgataaatt gatgatttat attttttacc
     7081 tgttcttctt ccatttaaaa tagctgacaa atagttttca ttaacaccta ttttcttcgc
     7141 taattccctt tgagtcatat ttaattcaat aagtctttgc ttgacttgca ctcccaaaaa
     7201 aggtatccat ttttttcgag catccattta tcaacttcct ttctaagtga tttatattta
     7261 cttttatatt attttagctt acgcaccccc tctatttagt atatattttt aatatgtaca
     7321 catattaagt atataaattt atttaatact aaataagatt ttttgattac tttgtgctaa
     7381 aattaggatg taaattcgta tcattaagtt aattataaag tcattttgac cttaagtcaa
     7441 tggtttttat gtcattttga cctttaattt gaaactattt acatcttaaa aagtcttttt
     7501 gacattaatt gaatggagag tgtttatgga tactttagga gaaagaattg tttatttgag
     7561 aaaatcaaaa aaccttaaac aatatgaact agaagaaatg ttgggctgtg acaacttaag
     7621 taaatttgag agaaacatta gaaaaccaaa ttatgaaata ttaaaatcta tagctgagat
     7681 atttaatgtt tcagtagact ggctattaaa tggagataat ctatcacata aatcagattt
     7741 aatttgcgat tcctcctcaa actatccatt taatagcata aactctaatg aaataaagct
     7801 tttaaacaac tttagaaagt taaatgatta tgataaagcc aaaatagaag gtatgattga
     7861 attaaaactt catgaatatg aaaaagagaa ggatttagga aaaattgagt ataacaaaaa
     7921 taaagatgag aaaatagata aataattttt atgttgcttt tgatacgatt ttacatcatt
     7981 tctttgttct aatattttta tttcatttat tttctttttt ataattttat gataaaataa
     8041 ttagtatatt ttgctttact tgaatgtaat tatttataca aaagtcaaac ataatataaa
     8101 tgtagtaaat ttttagtttc aagtaatatc tcgttattat aactaaatat ttattaaaat
     8161 taggaggata aaatgggctt aagagacaaa ttcgcgcaat catttgctag atcaaaaact
     8221 atgagtggcc ctgagaaaaa ggctaatgaa ataatgggta aattgcttct taagaaagct
     8281 atattaccta tcgttttaat gttcgtgata ataatcgctg gagctatgct taaaatcaat
     8341 tcttgggtta cacttggcat aaatttagtt attgctgttg gtgctttctt ctacataaga
     8401 aattctagta aaaagtatca gaactttaaa ccttatgttg gcaatttgat tagtttagag
     8461 aaaaaaggta aaaaagagta cgttgctatt ataaaacaag gtaagttacc agttaagtta
     8521 caaatagctt atggtggtga agaccttgaa catgttaaga aaaatcaaat ggttcagata
     8581 agttataatc cagatgctaa aatagctatt ttggttaata gacaataatt taataaaaaa
     8641 aaggagttgt cttaaaattg attttttcta atcaaattta agctcaactc cttttttaat
     8701 ttttatatat tatttttatt acttttttta attaatattt ttaaatatca aatccttctc
     8761 aataaaatag tgcactatct acaatactac tttatctttt cctaacaata tataatctgg
     8821 tgctactctt tcatgttata caccgttttg taacatacct tttataccag taacatcttc
     8881 cttaatttcc tctacatctg ttttcattat acccatttga acaagtatat ttttgtttat
     8941 ttcttgttgt tgtgtagata attctataaa attttctaca gtttttttat acatgtctct
     9001 atcttctttt ttctcctgca tagtattttt aaataataga gcacatagta tacctattgc
     9061 ccctagacta ctcaattcgg ttattaattt atccattata gcctcctatt gtctaaattt
     9121 tttatactta tagctttaat ttttgttgta ttcttacatc atgcaatatt atattttcag
     9181 gattatcaat ttaatcttct gtactttaaa ttgtaggttt aatt
//
LOCUS       DAGSWI010000154         9175 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.155, whole genome shotgun sequence.
ACCESSION   DAGSWI010000154 DAGSWI010000000
VERSION     DAGSWI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.155"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(71..253)
                     /locus_tag="MJF80_003341"
     CDS             complement(71..253)
                     /locus_tag="MJF80_003341"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281973.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            complement(342..503)
                     /locus_tag="MJF80_003342"
     CDS             complement(342..503)
                     /locus_tag="MJF80_003342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281974.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            complement(582..1082)
                     /gene="tcdE"
                     /locus_tag="MJF80_003343"
     CDS             complement(582..1082)
                     /gene="tcdE"
                     /locus_tag="MJF80_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBZ0281975.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            complement(1204..8304)
                     /gene="tcdB"
                     /locus_tag="MJF80_003344"
                     /gene_synonym="toxB"
     CDS             complement(1204..8304)
                     /gene="tcdB"
                     /locus_tag="MJF80_003344"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBZ0281976.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            complement(8622..>9175)
                     /gene="tcdR"
                     /locus_tag="MJF80_003345"
     CDS             complement(8622..>9175)
                     /gene="tcdR"
                     /locus_tag="MJF80_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBZ0281977.1"
                     /translation="QKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGET
                     DLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDEN
                     ILNMKNNYQSKSVFEEDICFFEYILKELSGIQRKVIFYKYLKGYSDREISVKLKISRQ
                     AVNKAKNRAFKKIKKDYENYFNL"
BASE COUNT         3081 a         1522 c          891 g         3681 t
ORIGIN      
        1 tattgatttt ttataattta agttttcata tttaaatcac ttcctaacat ttcaatttaa
       61 agatactcaa ttagttaatt gactcttcta ttagataagg agattgtctg atagtaattt
      121 gtaaacatat cttatatttt tttaatgttt ttatttcttt atatattttt cctataactt
      181 ttaaactatc aattaaatca tttattaagg tttgatactt attttctata tcattttgtc
      241 cttcatcacg tatttttgtg tttttgaata atttccatca caatttacac atatttctat
      301 gtttaactat tatagtttgt gatatctgta actaatgtag cttaatagat tttaccaact
      361 gaccatgcac catctttgtg aaccaatgtt tgatatacta ccttgtcatc tatatagtaa
      421 tgaacacttg cttcgaaatt atatgtattt aatacttgtg tgtctcttgg catctgctcc
      481 ctcgttttca ttattagttt catgaattat gataactttc agctattctt atttggataa
      541 cactttttat ttgttaatat ctttattata tctaccatta cttacttttc atccttagca
      601 ttcatttcat ctgtcattgc atctagtaaa attgctattt tttcctttaa tctcttaggt
      661 actggtaatc cacataagca catatttttt aatatactta cactttcata taaaataaat
      721 aaaatagaga aaaattcaga tattccaaga tgttttagtc ttaaaaaatt gatacaatct
      781 tgtggtaaca taaataaaaa gttaaacttt gtaagaatgt caactactga taaaaaaaat
      841 atacatgcta tcattgctac ttttctgatt cctccatcta ttccaaaact agaattaaat
      901 ttacgacttt ttattgcact taaacatcct aataatgtat caaatgatat agttaaaatt
      961 actaacttta taaatatatg ctctgataaa aaagatattg tcatattcat aacgcctcct
     1021 aggtttatat aaaaaaatat tttgttacca ttagaaatat aaaaaggtga actactgtgc
     1081 attcatcata gtccaccttt ttattggtaa atcttcatat tagatagaac actttatgta
     1141 gtgtatcatt ttgtttattt ttatatattt aagtataaga ggatatattt aacatatttt
     1201 tatctattca ctaatcacta attgagctgt atcaggatca aaataatact cctcaccatc
     1261 aataataact gaaccagttg ctgcaatata ttcatctgta aaataatatc tcttttcatc
     1321 taaatctaac caaccagtat agtttattga ttctccctca aaattctcat ccaaagtatt
     1381 ttgatgtgca aagtatttaa atccatctgg tgtattaaat actccaatct gcatgacacc
     1441 atcttcacca aaatagaaca tcttatcttc tatatttata taaccaaatt gcatttcacc
     1501 attctcatta aagtaataat tattattttc aaatgatata agacccgttc tcattatgcc
     1561 cttctcatca aaataatatt ttatatcatc aattaaatta atacctttgc atgctttttt
     1621 agtttctgga ttgaaataat atttatcact ttcattttcc atatcatata tccatccagt
     1681 ctcaattgta tatgtttctc caaaataata tacatcttcc ccaactctaa ctaaaccact
     1741 atattcaact gcttgtccgt aaatattatc atttacagta ttagcaggtg caaaatattt
     1801 atatccatct gaagtatcaa atacaccaat ttgaactata ccattatcat ctatatagaa
     1861 ataattgtca tctatgtttt gtactccaga ttctataatt ccagagtcag agaagtagaa
     1921 gactttatca tttatagtga caaatccaac ttgcataatt ccatcatcat taaaataata
     1981 ttgaccatca tttattaatg acaaacctat atatgcttct gctgtatctt catcaaaata
     2041 atactttgaa ccatcctcta aatctttcca tccaactaca gctgtaaatg aatcatcaaa
     2101 atagtaaatt ttattattga aatttaatat accagaatat gagatttctt caccttcttc
     2161 atttcctaaa tcttcattat gatgagcaaa atatttaaat ccatcttctg tattaaatac
     2221 tcctatttgc atttctccgt tttcagcaaa gtagaaatgc ttgccatcta tttcagtgta
     2281 acctactttc ataacaccag aatcatcaaa atagtgttta ttatcattta tactaacaaa
     2341 tcctttttgc ataactccat cagaattgaa atagtattta taatcaccta tttgatttag
     2401 acctttaaaa gctttacctg tttctgggct aaaatagtgc atttcaccat ctaattcttt
     2461 ccattctaca gctcctctat aattatcatc aaaataataa atattttcgt caataattaa
     2521 ttttccagta aaatcaattg cttctccttc taggttttca tcaagtgtat tagctggggc
     2581 aaaatattta aatccatctt ctgtactaaa tacacctgtt tgtaacactc cactttggtt
     2641 gaaataataa tttttgtcat caattattgt ctctccaatt gaagcagctc caccattaat
     2701 tggattaaag tagtatttat catcgcctac agtcacaaat ccagttatca aattatttac
     2761 tggtggttta aaataatata atgaatcatt aatatatatt aatccagata ccatcattcc
     2821 aaagttatta atataaaatt tctcattata taaagaaact agacccaaat catagccaat
     2881 caatccatcc tcataatatg aaggtgtaaa tgataagatt atttcactta caggtacatc
     2941 ttgtttatca ctaaagttaa aagatagctt atttgccaaa gtcttatctt taaaaacatt
     3001 aacgaatctt attttaactt gtgacacctt attttcttca ctagttgaca taagaataaa
     3061 atcattacca tcattactcc atacatatcg tatagataga tcattgatat taacatttat
     3121 tttttcattt atataatttg catctaatac aataacttct ggataagtat tatttgtttc
     3181 atatacaggc gttatattta tttcatctgt ataaatattt ggtgaaatta caactttatt
     3241 aacacaactg tctattccat aaagatactt ttgagagaaa ttgataacag ttgaagatat
     3301 atctccagaa tcatctaaat cataatttgg ttccactatc atattttgtc tatttcctac
     3361 atataaagta taattagttt ctagtgtatt aaacttaatg aaatatggtt gtatattatc
     3421 attttcatca caaataaact caaattggcc aatagaagta gtaccactta ttataaaatt
     3481 agcatctaat ataaacttaa tattagattg taagaaatta acgaaaatac tttttatatt
     3541 catactttct aaaaagctca ttaaagaatc tgaagtattt gtattacctt ttctattcat
     3601 gaacttcaaa atctcagcta ctccactttc atctaaatgc acactattta actttatagt
     3661 tttttcatct tgtagagtca aagaaagaga gatttttata tcatccttaa gataataacc
     3721 tgttaatata ttaacattat ctttagttat aactttgaca tctttcaaat tattactata
     3781 atatccaaat gaaggcttac tatcatccat ataaacctta cttataagaa ctacatcagg
     3841 taattcagat acaaataaac cttcttttgt tgaaccatta ataaaaccat tctcttttcc
     3901 ttcactatct acaaagctat atggtatatt tttctgtaat tcgctattga atcctatata
     3961 atctattttc tgttgaatat gatttgaatt taacatcaat atttttaatt cgccagaaat
     4021 aagtaattta tatgatttag ataataaatc aacttctata attgcattta ttccttctaa
     4081 aattgaaaat gttaaagaaa caaatccatt acttccattt acctcaccag aaaaattaat
     4141 ctcatggcta tttaagataa ttttattctc ttcaatactt agtgtagata aaataccttc
     4201 tattaaatca ccttttttaa ttttatcaga ttctatagtt acatctctca caacattatc
     4261 aacatctata atccaaacat cactttcact taattctata tttataccca tattatattg
     4321 agaaagagac aatgcataag ttcctcctga accatagaaa gaatatgata atttttctct
     4381 tatatattct gtagttatta ttggaactat aaaacttcta gtattactat ctaaatttat
     4441 tcttatatta gtatcttcat atcttggttt taatgttgtt attaaagcat cagctataaa
     4501 agcaaaatat ctccaataaa actcaccttc atagttatct cttatacggt ctaacagttt
     4561 tgtgccatca ttttctaagc ttcttaaacc tggtgtccat cctgtttccc aagcaaatac
     4621 tctatttgga gcattaggta ataccattaa atcttttgac aaatcaagtt cttctttttg
     4681 tacttccaat acgtcatata tagataagtg tggctctcta tatgttattg atggtgctga
     4741 aaagaagtga tctatatcat cagttacagt atgacctgaa ccaccttcca ttctccagat
     4801 ttcacattta cctaaaacta ttgaattatt attaaaatct atttctgata tcactaaatc
     4861 atcttgtggc atcattattt tatcatctaa taaagtaaat actccttcag tttcaactaa
     4921 tgaaacatgt ttaaaataat ctacaacctt tgttgcctta tctcgaagta caagttcatt
     4981 gtttactaag cttggtatac ctgctgaaat tcctgctaaa ggaactaaaa gtatactaaa
     5041 tccactagct acccccaaag atgaagtaat gattgcagtt gtagctgttg ttaaatttac
     5101 tgccattata cctatcttag cttctatttc ttgtcttaat aatgggtcac tcgtttcact
     5161 tagctctttg attgctgcac ctaaacttac accatctata atagttgcaa ttataggtaa
     5221 tccttcagat aatgtaggaa gtaagtctat agtttcatct aatgcagttg atactaattc
     5281 aacaactttg gctgcatctg taatagtatt taaaccagta ctaaataatt gagcgtaaac
     5341 ttggactttc attgctacac ttaaattact aagagattct ttagaactat tatattctat
     5401 taatgattgt ataaaaaatg cagcatttaa agtatttact tcgtgtgtag tatctaaatt
     5461 tacttttttt actaacttac catttacagt atcaaatata gtacctttta tcttagaaat
     5521 ctcttcagtt atatgattag catattcaga gaatattgtt ttttcagttt ctacaaatat
     5581 agattctcca gtttctttat taataaatct tatactaaat ccctcatcag tctctgatat
     5641 gtcctcaaaa gatataaaat gagaatcttc taattcattc tgttgtttta agtcacatag
     5701 tgcatcagaa atagattcta ttagtttaaa ttcatctttt atataattaa tagagtcaga
     5761 agttaaattc ttagcttctt caatcctttc ctcaacaatt tgcgtatcta tatttgaaat
     5821 aacatttatc tcacattctg ttaacattac tttttcttct agttcaatat cacttgaatt
     5881 agaattattt ctaatttctt gtaataatgt agatagctca ggtaaatttt tagattttac
     5941 tgtaatttta ttttctttag gattaaatga tatatattct tttgatgaaa tatcctttat
     6001 aatactttct tctttattta tccattcacc agaatgatcc aataattctc ttcttccttc
     6061 actatttatt ctaacttcat attgatttgc acttactata atagagtctt gacttataga
     6121 tggcattaat tctgatattt tatctttaac tttaagtaat aattttccag gataagtctc
     6181 ctctacgttg atagagtagc taaacatatt acatcctaat aaatttattt ctattgactt
     6241 aggagaaata tcctctttag ctaaatctat tgctgcttct atttctgtgg ataatgaatc
     6301 tacatcaaaa cctgcaaata tatcagtatt aaattcatct ttaccatgac caataaatgt
     6361 taatttaatc ttaggtctat cagaaattat acttggaatt ttatacttgt ctatttcttg
     6421 tatttcacca tctccaggat tataataata tgcaatttct gaatcttcta tatttttctg
     6481 aaatagtaca ctatcataag gagtctttgc aaataagtta catgctgctt cataactaat
     6541 tttatctcct tgtaactgaa caatatagtg tatatatcct ctctctgaac ttcttgctaa
     6601 tgaagatatt ttttctaaaa gatactcctt gtcaactact atattttgag aaaaatcaag
     6661 attatcatct tcaccaagag aaccttcaaa ataattcctt ttatattctt caaattgagc
     6721 tttagctctt gcatcgtcaa atgaccataa gctagccatt tcttgttcag ttgattgaga
     6781 aatattagtt ttagagattt caaagtttct taaatcagct tctatcaaat ggatattcat
     6841 actgccttct ttaaacatta ataaatcttg ataagctgcc gcatatgctt cagggccact
     6901 taagttaata gtagttttaa catctgggaa gaaaccaact cttaaatact ttcctagttc
     6961 catcataaat ctaccattat ctgcattagc ttcagccatt atactatcaa taaaggtatt
     7021 cgttgtagta ttaaaatcat tatcctcgct aatagctgga tttaaactat tattcaatat
     7081 tttatatcta ttctcgattt gttttactat taaattgcta caatatgagt ctttcacaga
     7141 aattagccct tgatttataa tacccttact attaaatgca attttaactt ctagtggtga
     7201 tgcctccata tcaccaagtg atgagaatat ttctgattta tctgacttag aagctagaac
     7261 agattcaaaa ctactttgaa cttcttcgtc taacatgtca aaatgttctg aggtatattc
     7321 tggtatatat tctttgtatt tcattatagc ttctaacttt gtcatttccc aaaaatccac
     7381 tgttactgaa ctaggtttct ctatagactc aaataagtct ggttgtattc ctggtaacat
     7441 atcaacatct aaatacatac caccaatttc ttttaatgca gatattctta atatgtcaga
     7501 agcagcagct aaattccacc tttctaccaa ctcttgttca tataagttga atgactctcc
     7561 atttttaaat tcttcaaagt ttctaacatc atttccacta ttctgtgtaa ttttatttaa
     7621 ggattcttca atataggtat taagttcatc tatctccttt gaatactcat ttgaaagata
     7681 tgtctttaca atatcatcaa ttataagttc aggattttct tctctttgag ctttatagta
     7741 gtttatgaaa tttttctgtt tatcataaat tatttccata cgttttctga agaatttatt
     7801 atagtcaaat ctagggtcat ttaagttttc tctaaatgat tcaagtgtat catttattgc
     7861 tgattctact acagtttttt tcaatgtgtt tatcaaaaat gcattactat cataaaaaac
     7921 attaacatta taatcactat ttacatcttt ccattgattt atataattaa tagcagtgtc
     7981 atttatttga cctccaatcc aaacaaaatg taaatttttc tcaactggag ttaaattatt
     8041 attctttagc tctaatactt ctgtaactag atattcctta aattttttta aggctttatt
     8101 tctaccagat tttttatatg tatctatata aatatctgtt aaactattta tatcttttaa
     8161 ttttaaatat ttttcgacta cagtattctc tgacatatta tgatattctt ctaaagcatc
     8221 caatattgca acatattcat cttcttgagt acgaaatctt acatttgcca ttttttctaa
     8281 ctgttttcta ttaactaaac tcataaaatt ttctccttta ctataatatt tttacatcta
     8341 aatgctaaaa ctcttttata tatcctcctt tctatttata aaatatacta atatctactt
     8401 aggttttcat atacattcac ttcctaacat tttaaattta agacactcag ttgattaatt
     8461 tgctcttcga ttgaataaag acgttgtctg aaataaatat gtaaactttg ttctatataa
     8521 aatataaaaa ttaagatatt cttagattaa gttattgatt ttacaataaa gttaattcta
     8581 aaatttgatt tctattgtaa atatttcttt aagctcatta attacaagtt aaaataattt
     8641 tcatagtctt tttttatttt tttaaatgct ctatttttag ccttattaac agcttgtcta
     8701 gatattttta atttcactga tatttctcta tcagaatatc cttttaaata tttataaaaa
     8761 ataacttttc tttgaatacc agataattct ttcaaaatat attcgaaaaa acaaatatct
     8821 tcctcaaaaa cagacttact ttgataatta tttttcatat ttaagatatt ttcatcaaat
     8881 tctacaaaat taaacttcat tttagaatat cttctagaca actcaaaagt cttattcagt
     8941 aatgatttat gaatatattt gactataata gcatcgcttt tttctgaaaa gctacttaat
     9001 ttaatatttt ttattaattc aataaaaaat attattaaat ctgtttctcc ctcttcataa
     9061 tgtaaaactc tactaagttt ttttactaat ggcttaaaca taaataaaat ttcttgcaaa
     9121 tcatctactg agttatttct tgctaaaaca attaattcat aaaaagactt ttgca
//
LOCUS       DAGSWI010000155         8820 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.156, whole genome shotgun sequence.
ACCESSION   DAGSWI010000155 DAGSWI010000000
VERSION     DAGSWI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8820
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.156"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            134..1498
                     /locus_tag="MJF80_003346"
     CDS             134..1498
                     /locus_tag="MJF80_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBZ0281978.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            1520..2011
                     /locus_tag="MJF80_003347"
     CDS             1520..2011
                     /locus_tag="MJF80_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBZ0281979.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            2284..2955
                     /locus_tag="MJF80_003348"
     CDS             2284..2955
                     /locus_tag="MJF80_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBZ0281980.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            3040..3906
                     /locus_tag="MJF80_003349"
     CDS             3040..3906
                     /locus_tag="MJF80_003349"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBZ0281981.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            3939..5441
                     /locus_tag="MJF80_003350"
     CDS             3939..5441
                     /locus_tag="MJF80_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBZ0281982.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            5571..6023
                     /locus_tag="MJF80_003351"
     CDS             5571..6023
                     /locus_tag="MJF80_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBZ0281983.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            6183..6986
                     /locus_tag="MJF80_003352"
     CDS             6183..6986
                     /locus_tag="MJF80_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281984.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            7044..7931
                     /locus_tag="MJF80_003353"
     CDS             7044..7931
                     /locus_tag="MJF80_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBZ0281985.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            7950..8798
                     /locus_tag="MJF80_003354"
     CDS             7950..8798
                     /locus_tag="MJF80_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBZ0281986.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
BASE COUNT         3537 a          850 c         1559 g         2874 t
ORIGIN      
        1 atatcaagac ctgttaaaaa acaacagcaa taaacaaata aaatagataa atatatccaa
       61 ttaaggtata attaagtttt atatgatata gttatatttg ataatttaca atagtaaaac
      121 ttggagggaa aaaatgggtg aaaagaaaat aaataatatg aattattcac ataataatta
      181 ttccaaaaag acaggtgaac caactagaaa aataaaaaat agccatatca ctcttaaaag
      241 taaagaagaa aaaatttgtt ctagttgcaa taaagaaaat aatactaagg ataattactg
      301 taaattttgt ggaaatgaac tttatgaaat agcatcatta agaccacttg aaacaaaact
      361 agacttgaaa tctaaaataa aagagctttc atatcatgca aataaaagag gtgtattttt
      421 aacaacattt acaaccatat ttatattgtt tattattgca ttgatattta aagctattat
      481 aactattcaa tttaatgata ttagttattt aataaatcca gcacatataa tcctagctct
      541 caacttgggt caaattagtg tatctatgtc taccatgatg ggttctgggt ttataaatgc
      601 caatataggt ttgttgatat tacttataat ccctattttg gtattattaa tatcaaacct
      661 aatttttatg agaaaaagat gtagagattc aaaaactgta ttagcaaatt ctctaggagt
      721 tggaattttt tatggattag ttttagctat tttatctatt tttactaatg taaaaactag
      781 ttcacatagt atgttagaat atggatatgc attagaatat agttatgaat tttttagtgt
      841 tcttttaaat ggatttgtat tagggtttat atgtacatac ataactactt ataaaaaaag
      901 ttatgaaaag gaaaatatgt atttatctct tttttcaaat tctataagaa cattcatatt
      961 aggttatgtt ttagtattag taatactatt ggtactaact atatctgata gtagttactt
     1021 aaatgaacta gatatgagta gttattcaaa tggattaaat ttatttacta tactcccaca
     1081 aatagctagt tatatgtggg cttttgcaaa tggaatttca gtaaccataa taaactcaac
     1141 agtatcaatg tttactttaa gttcttcatc tttatttgga gatactaaat taatgtttta
     1201 tgcaatggga gcattgtcta tgttaatatt attattaaat ggatacaagc taagatttaa
     1261 gtataataca gatagtataa gacctataat agtatttagt atatattatg catttttgat
     1321 gggaatattg gctttattta gtacatttat actagatagt aatattaatt tctttaatac
     1381 aactaattat ggaacaactt taataatgca atttaaagtc ttacaagcta ttgtaatttc
     1441 atttgtatat agttttgtga tatctttaat aggatataag ctaaattcag ctgattaaat
     1501 atgattggag ggaatttctg tggaaggaaa tactaataat aaagtagatg ataaattttc
     1561 taaggttaag aaaaagtttg gtagtggaaa atttaaagtt gaattatata aagaaaaatc
     1621 aaaattaaat agagagattg atgatattca agaaaagaaa gcaaagatat tcttggaaat
     1681 gggaatactg acatatcaaa aaattagaga agattctatt aatgataata gttttgataa
     1741 attctgtgat gagttactag aacttgataa gttaatttat gaaaaaaata tggaaattaa
     1801 tgaaatggaa atggcagaaa gtaatgtaac ttgtgagtgt ggttatgttg gaaatgtaaa
     1861 tgataaatat tgtgctgaat gtggtaaaaa atttgaattg gaaaatgaat gtgatgattt
     1921 tataatttgt ggttattgtg aaagtgagat agatacagag gctgaatttt gtccatgctg
     1981 tggaagaaaa attataatgg atttagaata atattgggaa acctatgtat agtatacatg
     2041 ggttttttta ttgaaattat ttaaatggac ttataacagt agttatataa aatgaaaata
     2101 aacaccaaaa aaatgaaata aaatttcttt taaatgtaaa aaacaagcta atatataaaa
     2161 taaatcagta aaaatataga tgcgagaaaa aaatatgaaa aaatattgca aagaaaaagt
     2221 tttcatgtta ttattaatac ataaatcaag aaaataaatt tcagaaacga ggtaatttaa
     2281 aaaatgctag ataaggtaaa gggaagatta atagtatcat gccaagcttt agaagatgaa
     2341 cctcttcata gtccttttat aatggggaga atggctaagg ctgctatgga aggtggagct
     2401 gttggaatta gagctcaagg agttgaagat attattgaaa taaaaaaagt tactggactt
     2461 cctgttatag gtattataaa gagaaactat gaagattcag atatatatat aacgccaact
     2521 aaaaaagaag ttgatgaact tttaacaaca gggtgtgaaa tgatagcatt agatgctacc
     2581 aatagagtta gaccaaataa tgaagactta aaagaactta taaagtatat aaaagaaaac
     2641 ggtgttctag taatggcaga tatttcaaat tatgatgagg ctatcaaagc acaagagtac
     2701 ggtgtagatt gcgtttcaac tacactatct ggatatactc catatactaa aacattagaa
     2761 ggtccagatt ttgtattaat ggaaagatta gttaaagatt tagaaatacc tgtaatagct
     2821 gaaggaaagg taaatactcc tcaagattta aaaaaggtct ttgagttggg tgttcattca
     2881 tcagtagtag gttcagcaat aacaagacca caattaataa cagaaaaatt tgttaaagca
     2941 attgaaatta attaaaaatt aatttataaa ttaaaaaatt attatattta actatatttg
     3001 aaaattaaaa ttggaggtaa agatatgaga acaacagata tgaagggaat ttattcagca
     3061 ttattagttt catttgataa ggaaggaaat ataaatgaaa aagggttaag acaaataatt
     3121 agacacaata tagatgtttg taaagtagat ggtctttatg taggaggaag tacaggggaa
     3181 aacttcatgc tttcaactga tgaaaagaaa agaatatttg aaatagctaa agatgaagtg
     3241 aaagaagaaa taaaattaat agctcaagtt ggttcagtta atttaaaaga agctgtagaa
     3301 cttgctaaat ttacaactga tttaggatat gatgcaataa gtgctgttac acctttttac
     3361 tataaatttg attttgaaga aataaaacac tattataata caataataaa ttctgtagat
     3421 aatagattaa taatatactc tataccattt ttaactggtg ttgatatgag tttagaccag
     3481 tttggagaat tatttgaaaa tgaaaaaata ataggtgtta agtttacagc agcagatttc
     3541 tacttattag agagaatgag aaaaacattc ccaaataaat taatatttgc aggatttgat
     3601 gaaatgatgt taccagctac agttttaggt gtagatgggg caattggttc tacattcaat
     3661 gtaaacggtg tcagagctag acaaatattc gaattaacta agaatgaaaa aatatcagaa
     3721 gcacttgaag tacaacatgt gactaatgat ttaataacag atatattagg aaatggctta
     3781 tatcaaacaa ttaaattact acttgaagaa caaggtgttg aggctggata ctgtagacaa
     3841 cctatgaaag aagcaactga tgaaatgaaa tcaagagcaa aagaaatata cagaaagtat
     3901 ttttaataaa atacttttag tatgggagga ttagtcaaat ggtaggattt acttggatag
     3961 attttgcaat actagtggtt tacttattgg cagttttatt tgcaggattg ttattttcaa
     4021 aaaaggaaat gaagggaaaa gagttcttca aaggtgatgg aactattccg tggtgggtta
     4081 catcagtatc aatatttgcc acattattaa gtccaatatc atttttatca ttagcaggaa
     4141 attcttatgg tggtacttgg attttgtggt ttgcacaatt aggtatgttt atagcaatac
     4201 ctttgacaat aaaatttttc ttacctctat atagtaggtt agaaatagat acagcatatg
     4261 aatacttaga aatgagatac gaaagcaaag gtcttagagt acttggagct ttgatgttta
     4321 taatatatca aataggacgt atgtctatca taatgtatct tccatcaata gttctggctg
     4381 gtcttacagg aatacctgta aatgtactga taatagttat gggtgtaata gcaataattt
     4441 actcatatac aggtggatta aaggctgtct tgtggaccga ttttatacaa ggaatggtat
     4501 taatagtagg tgttacaggg acattgttct atttaatagc aagtataaat ggtggttttg
     4561 gaacagtaat ggatactctt acaactggac acaagttctt agcaagtaat gaagttatgt
     4621 ttgataaaaa tatactatct acaagtgcat ttataatatt tgttggagct ggtcttaata
     4681 ctttttcatc atatgtatcg agtcaagaca tagttcaaag atttactaca acaactgata
     4741 taaagcaact taataaaatg acatatggaa atggtgtttt atcaatggga cttgcaacag
     4801 tattttatct aattggaact tgtttattta tatactatac tcaaaaccca gatttagctc
     4861 aaacagttca acaagaccaa gtatttgcat catatatagc ttatgaatta ccagtgggta
     4921 taacaggaat actacttgca gcgatatatg cagcttctca atcaacttta tcgacagggc
     4981 ttaattcagt tgctacaagt tggactttag atatacaaac tatcataaca aaagatataa
     5041 gtatggaaaa gcaaactaag atagcacaat atatatcatt aggtgtaggt atactttcta
     5101 tagctgttgc aatagtatta gcaaatggag aaataaagtc agcatatgaa tggtttaata
     5161 gttttatggg gctagtacta ggtgttttag caggaatatt tgtacttggt gcattttgta
     5221 aaaaagcgac taaaatggga gcatatgttg gattcatagt atcagcaatt ttagttgttt
     5281 atttaaaata ccgtatgcca gaagtaactt cttgggcata ttctctaata acaatcacga
     5341 cttctgtagt ggtaggtaca atagttagtg caatagaagc taaagttaaa gcaaaagtat
     5401 ctttaccaaa agctgagact actgtttatt atgaaaaata atatttaaat gataaattgt
     5461 cttatagagt gattagataa ataatttagt tactctataa gattgtataa tatactttgt
     5521 atataaaagt ataaaaatag actttataat tatataggga gatagtgctt atgatatttg
     5581 gaaatataaa tcatagtaag acatattcaa cattgcatga agatttactt agatgttttg
     5641 aatatgctaa agataattca ttaatagatt atgaaaaagg tacttataca atagatggtg
     5701 atgatatttt tgtgaatatt gtagagtata aaacttgtga aaaagaagat agattctggg
     5761 aagctcataa gaagtatata gatgttcatt tgatgcttga tggatgtgaa cgaatagata
     5821 taaattttat agaaaattta gaacaaaaaa tatatgaaga aaagggcgat ttcttgcctc
     5881 ttgatggtaa aaataatgga tacatggaac ttagaaaagg tgatttttta gtttgttatc
     5941 cggaagatgc tcatatgaca ggaataaaag ttttagaaaa agaaaatatc aaaaaagcta
     6001 tttttaaggt tttggttaga taatgataat ttatataacc actagggctg aaaatagtga
     6061 tttaactttc agcgattagt gagacttcgt aaattttagt tagattacta ttttcagcct
     6121 tagtttttta tgagaaaatt gaaaataatt aaaagcttaa aattttgtaa aggagttaac
     6181 ttatggaaaa tatagatgta ttcttattaa taggacaaag caatgctaga ggattgggaa
     6241 atccaaaaga gagcatcatt ccaaatgaaa attgttttga atatttgagt actgatgaaa
     6301 ttataaatat gagatgtgag ctagagactt cagaaggtga tggtactata gcacctgcat
     6361 tttcaaatga atggaataag cttactggaa ataaagtttg ttttattcac aatgcaaaag
     6421 atggttcaag aataaaaaac tggaatcatg ataataactg gtttctaaat gacactatag
     6481 aaaaatttaa tacaggctgt actaccttaa gtaaacatta taatatagaa aataaatacg
     6541 ttatttggat tcaaggtgaa agtgatgcaa aatatggtag tgatgtattg tactataaag
     6601 aaagtctaaa aaaaattgcg tataggctta aagaagaatg tatgattgat aaaatgtttg
     6661 taagtttaac tggttattgg cttggagaag atgaatattt tattagaaca agacgaattg
     6721 cagcagcaca ggagtcggct tgtagtgaat gtgatatatt atgtgtagga agcaaaattg
     6781 ctatgcagtt tcatgacaga ggattaacta ttgatgatgt tcattacact caagaaggtc
     6841 ttaatatgtt aggagaggac ttatgtaaaa atatttataa gtatcatata accaaagaaa
     6901 aaagtgtatt aagtgatact atagacttat cagaagcaag gaaatatatc tgtgaattgg
     6961 aaaagataag taaaagattt gtgtaatata aaattttaaa tagagttgtt aatttataaa
     7021 aataatttgt atggagatat gttatgaaaa attacgtttg tatagatgtt gggggtactt
     7081 caataaaata tggttttatt agagaagatg gatttataat tgataaatca agtcttgata
     7141 ctgaagcaaa ggaaaaaggt ggagaaggaa tattagccaa aataaaagat atagtaaaaa
     7201 aatatataga ggaaaatgaa ataagtggta tatgtatatc aactgcaggt atggttgacc
     7261 cagttgaagg taaaatacta tttgcattag aagaattaat tccaaactat aagggcatgc
     7321 aattaaaaaa agaagtagaa aaagagttta atataaaatg tgaagttgaa aatgatgtta
     7381 attgtgctgg acttggtgaa atgtggcttg gagcaggtaa agatgcaaca tcttctatat
     7441 gtttaacaat aggtactgga attggtggat gtattataat aaataataaa ctgataaatg
     7501 ggtttagtaa tagtgctggt gaagtaggat atatgaatgt aaatggaagt tcttttcaag
     7561 aattggcatc tactagcagt ctaattaaaa aagtagcaaa aataaaaaat ttgaatgaaa
     7621 atgacttaaa tggaaaaata atttttgatt tagcaaagaa taatgatgaa gattgtttaa
     7681 aagaactcga taatatgata aaatcattag cagttggaat cgcaaatata tgttatgtaa
     7741 taaatccaga agtggtaatt ttaggtggtg ggattatggc acaagaaaag ttcctaaaac
     7801 caaaaataga caaagcatta aaggaggttc ttatagagag agtttacaaa aacactaata
     7861 tagaatttgc aaagagacaa aatgatgcag gaatgcttgg agcattatat aattttttaa
     7921 acaaaatata aagaatagaa gggtttttaa tgagtatttt agaacaattg gaatcaccaa
     7981 cttttaaagt cacaaaatca gataaattat taatagctta tataaaagaa aatatagatg
     8041 atgtatttta taaaccaata tcacaaatag ctaaagagag caatattggt gaagcaacta
     8101 taactaggtt tgtaaaaaaa atgaatttta atggattaca ggattttaaa gttactttag
     8161 cacaagaaat atcaactatc aataaaaaaa atattataaa taaaaatata caaaatgatg
     8221 agccagcttt ggatacagca aaaaaacttt taagttcaaa tgtaaccact ttagagaaca
     8281 cagtagagat aatcaatagt aaggatgtac atgactgcgc aaggcttata ataaatgcaa
     8341 agaaagttta cttcataggt attggatatt ctggtataat agcacaggat tcaaactata
     8401 aatttatgag aataggtctt aattgtgtaa gttttgacag tagtcataca atgataatga
     8461 tgtcttccat aatggaagaa ggagatttaa taattgcaat ttcacattct ggtgaaacag
     8521 aagagattat aaaaactgta aagcttgcaa gagccaataa tgctaaaatt atatcaatta
     8581 ctgaaaataa aaattctgaa ttaaaggata tttcagatgt acacttatct tatgtatcgg
     8641 gagaaacagt actggaaact ggttcaatat cttctaaatt agcacaattt ttcattattg
     8701 atttagtata tacacaggta gtaaaagaac tttctaatga agctattgaa aggaaaatta
     8761 aaactacaaa tgctataaaa ttgtttaaaa atgaataaaa taagaaattt gtaatgtttt
//
LOCUS       DAGSWI010000156         8532 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.158, whole genome shotgun sequence.
ACCESSION   DAGSWI010000156 DAGSWI010000000
VERSION     DAGSWI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.158"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..290
                     /locus_tag="MJF80_003355"
     CDS             <1..290
                     /locus_tag="MJF80_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBZ0281987.1"
                     /translation="RNLKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQI
                     CNKCGYKNEEVKDLNIREWIYPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            730..984
                     /locus_tag="MJF80_003356"
     CDS             730..984
                     /locus_tag="MJF80_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281988.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1020..1343
                     /locus_tag="MJF80_003357"
     CDS             1020..1343
                     /locus_tag="MJF80_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281989.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLSQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1617..1769
                     /locus_tag="MJF80_003358"
     CDS             1617..1769
                     /locus_tag="MJF80_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281990.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            1963..2886
                     /locus_tag="MJF80_003359"
     CDS             1963..2886
                     /locus_tag="MJF80_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBZ0281991.1"
                     /translation="MVGAFVLPNYAITLSEMSNLWGNFTMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMALVSVLFFTYSIYLCATWKDKSLIADNKVETVKA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VEKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   3153..3900
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3153..3181
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4091..4243
                     /locus_tag="MJF80_003360"
     CDS             4091..4243
                     /locus_tag="MJF80_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281992.1"
                     /translation="MKNCRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            4363..5176
                     /locus_tag="MJF80_003361"
                     /pseudo
     CDS             4363..5176
                     /locus_tag="MJF80_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5228..5389
                     /locus_tag="MJF80_003362"
     CDS             5228..5389
                     /locus_tag="MJF80_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281993.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5717..6181)
                     /locus_tag="MJF80_003363"
     CDS             complement(5717..6181)
                     /locus_tag="MJF80_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281994.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
     gene            complement(6317..6634)
                     /locus_tag="MJF80_003364"
     CDS             complement(6317..6634)
                     /locus_tag="MJF80_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281995.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(6917..7213)
                     /locus_tag="MJF80_003365"
     CDS             complement(6917..7213)
                     /locus_tag="MJF80_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281996.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKNYLNRF"
     gene            complement(7232..7645)
                     /locus_tag="MJF80_003366"
     CDS             complement(7232..7645)
                     /locus_tag="MJF80_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0281997.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7629..7862)
                     /locus_tag="MJF80_003367"
     CDS             complement(7629..7862)
                     /locus_tag="MJF80_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0281998.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8081..8455
                     /locus_tag="MJF80_003368"
     CDS             8081..8455
                     /locus_tag="MJF80_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0281999.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
BASE COUNT         3408 a          909 c         1358 g         2857 t
ORIGIN      
        1 ttaggaatct taaactatct cgctcaattt ctgatgtgtc atggtcagag tttattcgtc
       61 aattagaata caaggctaat tggcatggta gacaaattgt aaaggtgggt aaattctttg
      121 caagttcaca aatatgcaat aaatgtgggt ataaaaacga ggaagtgaag gacttaaata
      181 taagagaatg gatttatcct agttgtaatg aaactcacga tagagatata aatgcaagta
      241 taaatatatt aaaagaagga ttaagactaa taacaattca aaataaataa cgtatgtaag
      301 aaccgtagga actacgggga tagcctgtgg agaattagta agacatatgt tagtatgcaa
      361 aattctgtga agcaggaaat ctgtgacttt agtcatggga ggttcaggct agtattactc
      421 attattgtat tcaaaagcta aagtggaaac ctagtgaata tatgaattta gaagttaatg
      481 agagagcatt agcagccgac tcaatactta ttaaaattga agatgaagaa gaagcaatga
      541 aagaagatga aagagagaaa aagagaggta gaagaaggta gaatttaaag ttaaaatagt
      601 cacattgcta atatatgaaa atgttacaat tttggaaata gatggatata tatgttgtat
      661 agtataatgt agataatata tcaccaagta aaaaacaaca aataaatcta aattatgagg
      721 ggtgtattta tggaaaataa agatattaaa cttacaatat gtatatttgc ttttgtaatt
      781 ggaataatag tgttattttg ctgtataggt ctaggtcaat ttaatatgga ttgtattata
      841 aaacaaaatg gtgggtcaat agctaccgaa caatacaaca tatatttaga acaatctatt
      901 tcacaatata gaaattttgg ttctatactg gttttactag gaggaatagg aatactgttt
      961 gataaaagtg tcagaaaata ctaaaaaagt atatttttat ttaggatgtg atgaatgata
     1021 tggcaagaga tgtatttagt gtaaagatga caccactaga agcacagaaa atggtagaaa
     1081 aaaatttgga agcagattta gtttctgttg atatttataa cctagaaaat aataagtcta
     1141 taatagttac aatctttgaa aaatattatg caagaagcaa tagtgatgca ggtttggtag
     1201 tgatttgtga aaatactaca ggaaaaacac ttgttaaaat tacctcaact ggtagtgcaa
     1261 ttggactatc tcagattgat tggggaacag gtaataattt gattaaaaga gtaaaaaaaa
     1321 tacttattga ttatattatt taattatttt atgtagatat tcttaggttg gtatggaatt
     1381 tgtactaacc tattaagtaa tcaataaaac taaataaaca tagaaagcac ttacaaatat
     1441 gtaggtgctt tttattgaaa aaatagaaaa taaaataaaa agtaagatat aggaaaaata
     1501 tgtaagaatt atatattata ataattgtag caagaagatg taatctacaa tttatagagt
     1561 ggagttcatg tattaaaaaa ttatcctccc aacgataaga agggaggtga atatacatgg
     1621 ataatttttt atttaatgtg ttagctagtg taacagctag ttacatagtt tatttgatta
     1681 gcaagttaat caaaaaagta aaaagccact ctaccgcaaa gagtgactta aagattgata
     1741 taaattttaa atatcatcgt aaaaaataac tttcagttat agaacttcac tctaccgcaa
     1801 atagattgta gttcttcttg cttttattat accacaaatt agaaaaaata ttgcatataa
     1861 tattttttat agtcaataaa aatatgaaat tttttataca caatgatgat gaatctaact
     1921 agatgaataa atttaattgt aaataagaaa gtacttactt aaatggtagg tgcttttgtt
     1981 ttgccaaatt atgctataac attgagtgag atgagtaatt tgtgggggaa ttttacaatg
     2041 tggatattag taccaataat aacaatagta ttacttataa tagctgtttc ttctatgcaa
     2101 tatattttag ttatgatagc ttttttgttg attatatatt cttttataga aaagaaaata
     2161 gttatggctc ttgtgtcagt tctttttttc acttattcaa tttatctttg tgcaacttgg
     2221 aaagataaat ccttgatagc agataataaa gttgagactg ttaaggcaca aagagaaact
     2281 gtagaaagag aaaaagaaat ggaaagaaga agaatacaag aagaagtaga caaagaaaga
     2341 tatattgaaa aacatggaat ggaaatatca gaaaatgatt taaaagttaa gctagaggct
     2401 ttagtacctc aagaatataa ggggaagaaa tatgaattaa aagtagaaaa atttaaaaga
     2461 tatagtatgt attttgattt aactgtacaa aatgaaaaat ttagtaattc tgaagaatgt
     2521 aaaaaatttg taaaagaaat tgctaatgat cttaaaaaaa ttaaaataag caaggcgtac
     2581 tttaagtttc attcaaaaga tgatggcggt atatataatt ctgtttatat tgattatttt
     2641 agaaacatac aaaataatgt agataatgtt gaaaatttag aattcaatga gtttgaactt
     2701 aaaaccgaag aagaagagaa gcgagaacaa gagaaaatag aacaagaaaa aaacagttat
     2761 aataactaca ttcaaaacag agtggttgac ccactagata gaataaagaa actaaaagag
     2821 ttgctagatt caggagcaat tacacaggaa gaatataata aaaagaaaaa agaattatta
     2881 gaatagataa tatagtaagc acttacaagt atgtaggtgc ttttaaattt acaaagtatt
     2941 ccattttaat tttatagttt agattttatg atataataaa aatatagaag ttttgcagtg
     3001 tgcgatattt gttacaaagt agggcttaat acttgaaatc taagatgttg agggtgcgtg
     3061 ataagtgtta tcaattgcac tattgcccgc tcactgcaat tttaagagta ttgtatatat
     3121 gtaggtattg gaaatgctaa gtttattttg gggttttaga ttaactatat ggaatgtaaa
     3181 tgaaattagt tgaactattg cttgatattc catatacgtt ttagattaac tatatggaat
     3241 gtaaatcaat aaacccaaaa acatttaaag aatacatgtt taagttttag attaactata
     3301 tggaatgtaa attgcttacg aatggttgga attacatcta ttgctaacgt tttagattaa
     3361 ctatatggaa tgtaaatcaa tatgattttg cattagattt tagtgaagga ttagcgtttt
     3421 agattaacta tatggaatgt aaattttctt taaaacagac atcaatttcg ttatatctgg
     3481 ttttagatta actatatgga atgtaaattt agaatcaaaa gcacttaatc ctagttcttt
     3541 catagtttta gattaactat atggaatgta aatagctttt tagcttcgtc tactacttta
     3601 taagcaaagc gttttagatt aactatatgg aatgtaaata aaagttgcac gcctatttga
     3661 tttttcaata cattatgttt tagattaact atatggaatg taaatgacaa agcgactatt
     3721 actatctcag gtgataaaaa tgttttagat taactatatg gaatgtaaat gttcctgttg
     3781 tgttttttac taatcccatt ttatatgttt tagattaact atatggaatg taaattgctg
     3841 tgatttcact aataaaacaa ttaataattg tgttttagat taactatgtg gattcaaaat
     3901 taaataaaca aagaaagcac ttacaaatat gtaggtgctt ttattctgct caaaattggt
     3961 cggttgggta aaatcattag aaaaaattag taaaaacctc ttttctgtaa ctcgttacaa
     4021 tattattatt aatgtaacga gttacagaaa agaggtgaat aaaatagcaa ctaaaagtag
     4081 ggcagagtat atgaaaaatt gtcgaaaaga taaaagaggt tttagtgtac ttttagacaa
     4141 agaaaagtta gataaatttg atgaagtatt agaggaaaag aatctaacca agaaagaatg
     4201 gctagaagaa aaaatcgacg aggaactgga acaaaaggaa taaaaaataa gggtcactcc
     4261 caccgaccaa agtttgagta acccctatga tgtatactat cgtatatcaa ttataatata
     4321 tgtcattcct taaaaaaatc aattattaag gagtgtaata ttatgaaaaa tgaattaatg
     4381 atgtttgaag gaaaagagat tgaagtattt gaatttgaag gaagaatttt attcaactca
     4441 aaagatgtag caaattgttt agacataaaa aatgttaatg agaatataat acttatgaat
     4501 gaaaagcaag ttgtaaagtt aagaaattcg gatatcagta atactgacat ccgaaaactt
     4561 aataatgctg gagaaaattt tcttactgaa agtggagtgt ataaattaat ctttaaatca
     4621 agaaaagaag aagctgagag attccaagac tggataagcg atgaagtttt accaagcata
     4681 agacaaactg gtgcatacat aactaataat gtaatcccga aaagttgaga gaaaaagcaa
     4741 gtgagattga aaaattacag ttgcttataa cagtacatct atgttaaaag aattgctaga
     4801 tgatgcaggc tttgacaata aatcaaaact attaacagct aaaacattat ataagaaagc
     4861 agaaattgat ttaccaatag agataaacga agaagaacat tattttgata caaaacaaat
     4921 agcatctaaa ctgaaaatat attctaagag taataaacca gctcagatgg ctgtttgtga
     4981 gattattaaa aagattgatt tagaagatgg tgaagttaaa ggtgtttggg aaattaatgg
     5041 ttcttggact ggtactgtaa ataaatatac aaagagtgta atagataagg tgagaacttg
     5101 gatagaggaa aataatagac ctaccaagat tgcaggtgag aagaagaatt attatgtatt
     5161 ttacaaaatt gagtaaattt atcagttgta ttaaataatt tagtttagtt ttggggggat
     5221 taatacaatg tgtgagaatt tacttgataa tatgtatatt gaaaagagaa aagaagaata
     5281 tagaattaaa cttttgaaaa taagagaaac agatatagat atatataata agttagaaag
     5341 tatagtatat aaactttctg agaaaaaatt agagaaaaat aataaataga catataaagc
     5401 acttggatat tctactgttt caagtgcttg tttggtataa aatggtataa tggagataga
     5461 gaattatatt aaccaaaaaa tggagaaaat agagaagatg atttagttga cgctttatca
     5521 gtaggagtac ctgcaggtaa gacagaatct ctagtacgat tagttggaga tggaataagt
     5581 gatactcaaa aaagtttaat aaataaaaga gaatttagaa atataactca aattggtgga
     5641 aatggaaatg agaaagcatt tagtgaagta gaaaacttag ttaaatagat tagaaggtag
     5701 caactagagt tggttgttac cttctttttt tatactttct ctctctaagt attttatttt
     5761 tcatatcttc taatacatct aatgcagtag tataaaattg tatattattt ctataaggta
     5821 tagctattgg aagaatgccc ttgttaacta aaaaaataag aagtacaata aataaaagtg
     5881 tcataatcat tctaataaaa taattagtga tatctgataa atcaaatgtt aagttaattg
     5941 ttgtagaaaa tacaacagcc ataatagata tatacaaagc aaaaatgggg ttactatttt
     6001 tttgtaactg tcctttaagc ctagattgct ctatttctat atcttgaata ctttttatgg
     6061 aatccatttc actataatac ttataaactt ttaaataata ttttttatac ttgtttaaag
     6121 cttcttcatt atctttaagg tctttgtcac ttaactctga tttaacttcc ccaaaatcca
     6181 taaaatccct cctacaatgt tatataatat actttaattt atagcatatt taagaactta
     6241 tatcagcaat ttgtcgaacg attttagcta aaaatagagt ataatgatag gcttattatt
     6301 tataagatat tattttttat atagttatat atttctttct aattagataa tataatgctg
     6361 ttgttataat ggctactaat attattgcta ttattgtaat atctaatctt cttgtaaata
     6421 agcctattgc tatatactca agacctgtta catagcgata aagtacttct agtttaacag
     6481 tttttttctt atctatattt ccatatattg attctaattt attaaaatat tttttactac
     6541 aaaaagcagt tttaggttta attaaatata ttattgtagt tcctattata actattattc
     6601 caataagtat aagattgata tttccaaaat ccatagcaga tacctcctat taaatttgct
     6661 tttcttaatc aaagtatatc agtaacacaa gtacaaagag tatctaaaat attacaaata
     6721 ttaacaggat agataaaact atagtaaaaa ttgtaataat aaagcgaacg aaaattatca
     6781 tactaaaaca taagacatgt tataattgta ttagatagaa gattaacata caccaaagca
     6841 ctccttataa taaagagtgc ttattttttt gaaattcatc aatatataaa ctatcaagaa
     6901 ccttattcaa cacaccttaa aatcgattta aatagttctt tttgataaca taatttatat
     6961 gatataataa aaaaatagaa gtgtaccacc aatacacttc tatagctata actaagcatt
     7021 ctcttgctgg gggagtgctt ttttcatttc tccaaacaac attatttact tcatcagtaa
     7081 ttatcaaatc aatctcatta tctttcaaaa tctctgttgc agtacaaatt agattatcta
     7141 agtcaaaact tcgagttatc tctagttgtt tgttatttac ttctcttttt agtatataag
     7201 ttttaaaata catttatatc accccttgta tttactcttt tttctctaaa aatatattga
     7261 taagtaaata tgcaacaaat aaaaaaatct caatgacaca tagtgttata gctataacta
     7321 gaagtgattg atttttccat gcactataag ctatgatgaa aagaaatgta aaagtaaata
     7381 atgtataatt cataatttta actaagatat gacttgtttt cattgttaag tacaagtctc
     7441 tttcatctgt actatcgtgt aattcttcaa caatgccttt tcttgaaaaa gcattacaaa
     7501 aattaaagat tgctattaca attaaaagaa atgatgaaat taggaatctt ttatctttta
     7561 aaatgcaata aataactaaa ctagcacaac ataacacaca aactataata cccactataa
     7621 aacttcgttt actctttact ttcatgtttt ttatcctcca tttctttatt ttcttttaaa
     7681 caacatagtt cttcaatcgt tgtaccaaac acttgtgcca tacgataggc aagcataaga
     7741 gaagggctat attgctcttt ttcaatagaa ataattgttc tgtttgatac atggactaaa
     7801 tcagcgagtt gttgctgtgt tagacccata tttgttcgaa attctcgtac ttttgttttc
     7861 attccataac ctctttctat atgaagctaa cttcatgtga agttaacttc ataatatcct
     7921 aaaatgagta aaatgtcaat ctcattagat aagtattata aaacctaaaa gaataatatg
     7981 gtaaaaattg gatggaatta tttaccttaa ttctcttgtt tgtaaaactt tattgatata
     8041 gaatttaaca tatacataaa tcggcaaagg ggcgcgaatt atgaaaatta gtaaattacc
     8101 agaagcggaa ttaaaagtta tgagatatat ttgtgaagct aacaaggtat taacttctag
     8161 ggaattagtt gctgcaatgg aagaaaaata tgagtggaat gaatcaacta catttacagt
     8221 tttgaaaaga ttagagcgaa gagagttttt aagtacagag aaaataggca aacttacaca
     8281 ttataatata ctagtaaaag agaaaaaata tctaaggttt gaaacaaagg aatttttgaa
     8341 gaacatacat aaaaattcaa tttcaagttt aatgtcagcg ttacatggtg aagacgacga
     8401 agtggatgaa gataaattaa gtgaactcga agaatgtttt aagaatttga aataagaaaa
     8461 tgacaaattg atacataata aataaaatga aggtatgaaa ttataacttt ttctaccttc
     8521 attttgttta tt
//
LOCUS       DAGSWI010000157         8508 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.159, whole genome shotgun sequence.
ACCESSION   DAGSWI010000157 DAGSWI010000000
VERSION     DAGSWI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.159"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            124..1983
                     /locus_tag="MJF80_003369"
     CDS             124..1983
                     /locus_tag="MJF80_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBZ0282000.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2257..2856)
                     /locus_tag="MJF80_003370"
     CDS             complement(2257..2856)
                     /locus_tag="MJF80_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBZ0282001.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2959..3756)
                     /locus_tag="MJF80_003371"
     CDS             complement(2959..3756)
                     /locus_tag="MJF80_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBZ0282002.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3869..3974)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4066..5052)
                     /locus_tag="MJF80_003372"
     CDS             complement(4066..5052)
                     /locus_tag="MJF80_003372"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBZ0282003.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5536..7266
                     /locus_tag="MJF80_003373"
     CDS             5536..7266
                     /locus_tag="MJF80_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBZ0282004.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7376..7456
                     /locus_tag="MJF80_003374"
     tRNA            7376..7456
                     /locus_tag="MJF80_003374"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7410..7412,aa:Leu,seq:tag)
     gene            7791..8306
                     /locus_tag="MJF80_003375"
     CDS             7791..8306
                     /locus_tag="MJF80_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HBZ0282005.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
BASE COUNT         3062 a         1057 c         1303 g         3086 t
ORIGIN      
        1 aacaaggttt atatattcta atagtataga aaaatttatt gtaaacgcat gtatattatt
       61 agaaaaaata gtttgaaggt attcaataaa cataaaaaac aatatagggg ataaaggtgt
      121 tttatgaaga gatttttgag gagaattatc ttagttttat ttattttgtt gatttttata
      181 agttttattt ctattaaatt aattcataat gttggagatt atggaaaatt gattaattat
      241 gttgggattg ttcgtggagc tagccaacgt ctaactaaac tagagatgaa tcatcaacca
      301 aatgatgaat taatagaata tattgatgaa atcctacagg agttgattac agggcatgga
      361 gattatggtt tagtaatcac tgattgtaat gaatacaatg aggacttact tttattagaa
      421 aaaaagtggg aagatttaaa tattgaaatt aaaaaagtaa gaatgaaaga acagaataac
      481 cagttattaa gtataagtga agagtttttt tctcttgcaa atgatacagt gtttaaaata
      541 gaaaactttt ctaaagaaaa aagtaattac ttaatgactt taataataat tatatctatt
      601 ataggtatat tagcatgtat catattgatt ttacaatata gtaaaaaaat ggttagactg
      661 gaaaaattaa atgtagattt aaaaaatatt gcatataaag atgaactaac aggagtaaat
      721 actatagaaa aatttaaatt ggatgcaaat caaaatattt gtatacataa ggataagaaa
      781 tttgctgtat tctatattga ttttgaaaac tttaaataca ttaatgatat atttggatat
      841 gattatggtg acatgatact gaaaagatat gctaatctta tgatgaatga catagggaaa
      901 tatgaaatat ttgcaagaga aattgcagat agatttgttg cgttaagatg ctatgtagat
      961 aaggaagatt tagtggtaag acaacgagat gtagacaatg aactaattaa tataacaaat
     1021 gaaattaaaa ataaacatag tattactgtt gttagtggaa tttgttgtat tgaagatgta
     1081 aatgaaaaat taagtataga tggtttaata aatagagcaa attttgcaca aaaaactgtt
     1141 aagaataaac caggtactaa ttatgctttt tataatgaca gtattcgtaa aaaaatgatt
     1201 gaagaaaata ctatcaagag tagaatacat gaagctattg aaaaaagaga atttatagtt
     1261 tatttacagc ctaaagttaa tttacatagt caaaaaataa attgtgcaga agctttagtt
     1321 cgttggctta ctccggataa aggtattata tctccagcaa tatttattcc agtacttgag
     1381 aagaattttt ttattgcctt agtagataag tatgtttttg aggaggtttg taaatggatt
     1441 agaaagaggt tagatgaaaa taaacctttt gttcagatat cagttaatgt ttcaagaata
     1501 caattttata acactaaatt tgtagaaact tattccaata tacaaaataa atacaggata
     1561 cctaaaaata ctatagaaat tgaatttaca gagagtgttg cttttgagaa tcaaaaacat
     1621 cttttggaaa ttatacatga tttgcatgaa aatggattta cttgttcttt agatgatttt
     1681 ggaaaaggtt attcgtcttt aagtgtttta aaagatttac catttgatgt tttaaagctt
     1741 gattctatgt ttttcaaggc tagtttagat aaagataaag agaaaatagt aataaaaaat
     1801 attgtacata tgttaaaaga acttaatatc acaactgttg ctgagggcat tgaatgcaag
     1861 gaacaagttg aatttcttag ggatattggg tgtgatttag ttcagggatt tgtattttat
     1921 aaaccaatgc caatattaga atttgaaaaa atattggata aagagtttgt ttataatagc
     1981 taaaatgtac tttataaaat atgaactaat taagtaaaat tattttgtac tgaataaatt
     2041 tttactttat ttacagaaaa gcgcgaaaag tgtattcctt taagtgtggg atggatagca
     2101 caaatagagg agaaaaaata ttggccatat aagaatgcat aataatataa atatttgaaa
     2161 ggtcagcatt atataaaaat taaaaagaaa ataaataatt tttctataaa aaagagacta
     2221 tgagaaaatt tactcatagt ctcctaaata atgtatttat tttcttcctg caacccattt
     2281 taatccccaa tgaaattcag tatccatttc tctatgacct ttgaagtatt taactacttt
     2341 ttcaacactg aatgtctcat catttacatt ttctatcaat gctattgaac acatattata
     2401 gccattttta tgttcatcaa gtttgacaac taagtctgaa tcttgttttt gtttgattgt
     2461 tacaattcca tcagcttcag accagttagc tacaccttcg tatataaaag cataaataag
     2521 tattcttttt atatctttta ttttattacc atttattctt aaattttctc cttgtgtatt
     2581 acttcctgtt ctgtcatctc catctaattg agcaaaaggt ggattgtcta aactaccaaa
     2641 ggcatttcct aatgcttgaa cagctccttt tacaccattt ttcatctcaa ataggcaacc
     2701 caaatctaaa tctatattac tgcttcttaa agagccaaaa aaaccctttt tttgtacttt
     2761 ttgattccaa tttaaattaa ctaagatttc acctaaatcg ctattttcct tttttgtaag
     2821 gtttattttt tgtccctttt ttaattctat agccatagta atcttcctcc acaaacatag
     2881 atttatttaa atggtagcac ttaataagtc aatattcaac aagattagaa taataatatt
     2941 aaaattatac taaatatact aaaaagtagg aatcacaata cctttgtatt tttcttcaat
     3001 taagcttctt gctttatctg atgtaagaac atcttgtaga actttaatct tttcactgtc
     3061 tttatcttca gttctaaatg ctaaaagatt tgcatactcg tcatttatag gagctgtata
     3121 aattccgttt ttcttggcat caagaccagc aggtacagca taagtagtat ctataaatgc
     3181 agcagtcaca tctggaagag aagatggaat ttgagcaata tctaactctt tgaattgtat
     3241 tccctttgga ttttctatta tgtccttagg actaactaat ccatcccctt taggtaacgt
     3301 taaaagtcca atactttcaa ggtatctaag gttcttagaa caagaagatg ggttattgct
     3361 tattgcaatt gtatctcctt ttttaaattc atcaacagat tttatcttat aagagtaaag
     3421 tattgctggg catacatata gttttttacc agccattatt ttgtaacctt tggccttaac
     3481 agcttctttt agataaggtt catgttgaaa tagatttgca tcaatttcac cattatttaa
     3541 agcttcgttt ggaagtatgt aatcatcaaa gtttttaact tctaaagtat atcctttttc
     3601 tttaataagt ggttttaatt cttctaataa ctctcctcct ggaactaaag tagcaccaac
     3661 aacaattttt ttatcatctt tagctttaga acatccaacc acagctaaag ttaatactag
     3721 acataataac aaacataata attttttgaa tttcatataa attccctcct gaatataatt
     3781 gattagttaa aaataaaata ccattcttta tcacactgca aaataaaaat aagcctgagt
     3841 ttaaactcag gctttaaaag ctaatttaga ctcatctttc agtataatac tgcaggattt
     3901 agcaccaatg tatataaaaa tatactggtt gctgggtttc atagggccag tcccttcacc
     3961 actctggata agaagtatca atttttaatt atgttataaa tattatcatg attttttacg
     4021 tctgtaaaca taattttata aatattttaa aatctttaat gtattttatt taacacctaa
     4081 aattccagaa actagaatat cagtatcagc acctaagaaa tagtcattta tttcaataga
     4141 atccagagta tttctcaaag cattttcttc atgccttata ccaataagtt tattttctac
     4201 aaatgcaaca tcacattttc caaaaaaatc tccaaaaaat tttatactag ttattatgcc
     4261 tttatcaaca tttaaattaa actcaacatt tccaccagaa tattttaatt catttgaaag
     4321 agtatatttt ggtgaattac caaaattcca atcccaagtt tcgtattttg atttagtaag
     4381 cttttctatt tgttttatat catgctcact caaacaataa ttagtattat cagtatttgt
     4441 tttagaaaga taatccatta tagcatcttt aaattctaat atgtccattt ttgaatgtaa
     4501 atgttcactt atattggtta cacgagattt tacagatttt attccttttc cttcaaattt
     4561 tataggttta acctttaatg cttttgagag gtcatttatt tcagatgaaa ataaaagagt
     4621 accatgatgc attactttgt ttttgtagtt gtattgagca tttccagaaa atttcttgcc
     4681 atctataaga aggtcatttc ttccagaaaa ttcggcattg acatctagtg ttttaagtaa
     4741 atctattatg ggctgagtga atcttctgaa atcactaaaa ttattattgt tacaagctat
     4801 aaatgtaaaa tttatatttc ccaaatcatg aaatacagct ccaccaccag actgtctcct
     4861 aacgacaggt atagaatatt cttttatata ttctaaattt atttcagaaa gtgtgttttg
     4921 gttttttcca acaattatag aaggttcatt cctccataaa ataaataagt cttcatcaaa
     4981 attttttaga agatattctt ccattgctaa attaaaataa gggtttgttt tttcattata
     5041 aattaataac attaaagtag ttcctccata gaattgataa aatttttatt atgaatacaa
     5101 ttaattttat aattcgacaa taatataaaa actcctttaa attagaaatt ttaaaaacta
     5161 ccttgcaaag tttaaataaa taatatatac tcaaaatatg tttaataaaa gtgaatagaa
     5221 gggtagaggc gcaataatct atcagtacta ataaggagtt aagcacagag atttattagg
     5281 aaaggagata ttgccgaagt tttataatcg atgctttaag attatattgc tgggggtaca
     5341 tagaaaatat gtacaactgt cacaggttta tttgtggtga gctatcattt aaggaattat
     5401 attatattta tttttactat tgttgatata ttgtaatgtt taagccttga gtgtttacca
     5461 ctcaaggctt ttttagtttt attgtaacat aataccctgt acagttcaca aaattaaaag
     5521 gagagggaga tttatatgag gaacaaaaaa attaatatta tttttctgac gacaatcatg
     5581 ttcataatgt caacagtaat ggtttttgca gaagaagata tagacactat tgcactagcc
     5641 aatgcagaaa aatttgggat tttaacttta ataccaccat tagttgctat aatacttgca
     5701 tttataacta aaaacgtaat aatttcttta cttataggta ttctttctgg tagttttata
     5761 attaaagcta gtggtattaa tgtttttgcg acatttatac aggcattttt agatttagta
     5821 gacagagctt tagtatcttt agcagaccct tggaatgcag ggataatact acaggttttg
     5881 gcaattggag gagtaataaa tcttgttgct aaaatgggag gagctaaagc tatagctgaa
     5941 gcacttgcta aaagagcaaa gtcagcaaaa gggacacagc ttataacatg gtttttgggg
     6001 cttttagtat tctttgatga ttatgccaat tcactaatag taggtcctat gatgagacct
     6061 gttgcagata aaatgaaaat atcgagagaa aagctagcat ttataataga tgccacagca
     6121 gcaccagttg ctggtttagc tataatatct acatggatag gtcttgaggt tggtttaata
     6181 catgatgctt ttgaaagtat aagtatagat gtagatgcat ttggtatatt tttaaataca
     6241 ataccattta gattttacaa tatattaata ttagctttca ttgtaatatc agcattatta
     6301 ttaaaagaat ttggtccaat gagaaaagct gagataaaat ctagaagtag aaaaattagt
     6361 atagaccttg atgaaggcgt agaagagttg gatgatttag ctccaaaaaa tggagtaaaa
     6421 ttgagtgtat ggaatgctat aatcccaata ggaacattga taatagttgc attagcatca
     6481 ttttattata gtggatatac atctataatg ggaggagacg ataaggctct gatacaacta
     6541 tttacaaatt ctccatattc ttttgaagca ataaaagaag catttagtgc atctgatgca
     6601 tctagagcat tattccagtc tgcacttgtt gcaagtttag ttgctattat aatggctgta
     6661 gtaaaaaaga tttttacaat atcagaagca attgatgttt ggatagatgg aatgaaaagt
     6721 ttagtaatca ctggtgtaat attaatactt gcatggtcat taagttcagt aataaaagag
     6781 ttaggtacag ctaaattttt aatacaccta ttatcaggtt cactaccacc attcttatta
     6841 ccaagtttaa tatttggttt aggagcaata atatcttttg ctactggaac agcttatgga
     6901 acaatgggta tacttatgcc acttgcaatt ccattagcat actcattaaa tcctgatatg
     6961 tcttatgtaa tagtaagtac tagtgcagtt ttaacaggtg caatatttgg agaccattgt
     7021 tctccaattt cagatacaac aatactttct tcaatgggag ctggctgtaa tcatatagac
     7081 catgttaaca ctcaaatgcc atatgctata tttacagcag taatcactat tgtatttggg
     7141 tatataccag ctggacttgg attgccaata tatatagtat taccagtggc aatagctgct
     7201 atatttgtag gtattcaaat aattggtaag aaagttgatg aagcggaaat agaacttgta
     7261 gaataaagat ataaataagt ttcacctttt attatcacta ttttgttagt ttttagataa
     7321 ttttaaaaag ttattgaaaa taatggcgaa atatagtaaa ataaaagagt gataagcgga
     7381 tgtggcggaa ttggcagacg cactagactt aggatctagc gccattggcg tgggggttcg
     7441 actcccttca tccgcacttt acagtactta attgtactga aaaaaactgg ggttgctggc
     7501 tttcggagtg ggctatcccg tttttatttt tttgtatata taatagattg gcactttagt
     7561 ataaggtgct ttttttatag gcaaaaacag tacatttatt tctgttttat tatcattcat
     7621 ctattagaat atactttagt taagaatgtg ttaataagca gtgttcacat tcttgataaa
     7681 atgtggaata atatgtattt ttactaagac ggaaatgaaa taattaaacc attaaataat
     7741 attataagat gatgaataag tagataaatc aaattttgta ggaggatatt atggaaaaat
     7801 atatatggtt gtttccatta ctttttattt ttcatgatat ggaagagata ataggttttg
     7861 gaatatggct taaaaaaaat aaatcaatgc ttgataagaa gtatccattt atcagtaaag
     7921 tttatgaaaa ttatagtact gaaggcatgg catttgctgt gtttgaagaa tttattttat
     7981 gcattatttt ctgtatactt acagttataa cagagaatca gtatgtatat ctactatggt
     8041 taggtagttt tatagcatat accttacatt tggtaattca tatagggcag tcaatcataa
     8101 taagaaaata tataccttca cttattacca gtatcatatg cttaccaata agcatttggt
     8161 gtataagtaa gagtatttat atagttgatt gtgaaatgag tacaacaatt ctgtacagta
     8221 taattggcat tataattgta gcattgaatt taaagtttgc acagtcgctg attggaaaat
     8281 ttacaaagtg gatgtcaaac atataaattt tagtttattg gacagttcaa ggaatcagaa
     8341 attggaattt tatagtatta atattttatg gtattaatat aatgtataaa attttatagc
     8401 aatgtatttc ataagtataa agtgactaga attttaagaa atatacaaat tctataaaaa
     8461 ctaaataatt tcaagtatat tttaaaaatc ttatttttca caagttta
//
LOCUS       DAGSWI010000158         8392 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.160, whole genome shotgun sequence.
ACCESSION   DAGSWI010000158 DAGSWI010000000
VERSION     DAGSWI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.160"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            56..1507
                     /locus_tag="MJF80_003376"
     CDS             56..1507
                     /locus_tag="MJF80_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBZ0282006.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1705..2346)
                     /locus_tag="MJF80_003377"
     CDS             complement(1705..2346)
                     /locus_tag="MJF80_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBZ0282007.1"
                     /translation="MTDNYIFDSYNNLVDGLSAYLGEGYEFILYKFNGVHYSINKIAN
                     GKYNTVKEGDIITDFDLALINKLENDADGDFISYFNRNKLGEPLKSTDIVIRGVDNKI
                     IGLLCIHFYLNTPLTRIIGSYIPEHNAMQSAPVLENIVEDTDEVILKSYQDAKVIVEN
                     DSNISSNDKNKSIVSILNESGIFKIKDSVVKVADLMGLSKNTIYMHLRNLNKN"
     gene            2820..4199
                     /locus_tag="MJF80_003378"
     CDS             2820..4199
                     /locus_tag="MJF80_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBZ0282008.1"
                     /translation="MAQDKIVVEILENIGGKENIKEVAHCMTRLRIKVCDSSQVNQEK
                     LKKIKGVLGLVLKGEQYQIVIGPNVSQVYNTLNEVIGIIPEKDSKKTISEGKQNSIAK
                     ILDTIAGIFNPIVPALAGAGLIKAILALLVASKFLSADSETYIILNTISDGVFSFLPF
                     MLAYSAAKIFKMNTYVAVAIAGAMLHPNFTNLIQQGLTKISFIGIPMSLIQYNGSVFP
                     IVLTIWFASYIEKGVDKITPKALKIILVPALTILFAAPIALIIIGPIAQMLGNYIAGG
                     ASILFERGGIFAGLIYGGIYSFMVVTGLHHGMVPVLVDGITRYGFNYISPVSGSSNMA
                     QAGSAFGVWLKAKDKDLKAVAATATISALTGVTEPAIYGVNLRLKKPFLCAAIGGAAG
                     GGFAAYFQCKAFAMGGPSFLTLPMFIGEGGHVGLVAIAFTIAFIVSAIATYIVGFEEE
                     VTADSHANL"
     gene            4243..4977
                     /locus_tag="MJF80_003379"
     CDS             4243..4977
                     /locus_tag="MJF80_003379"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010990049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBZ0282009.1"
                     /translation="MKIYLTKDYEEMSQVAAQMVLKYMYSNDNKRINLSITAGQTPKR
                     TYEILSEFVKNKPYFENVHYYNFDEIPIKGEKVGVTIRNLTNMYFREAQIPEERIHVL
                     DENNYQGFDRKIEADGGIDMVLMGLGWDGHFCGNLSGTVNSFDEETRAVSTYITDDMA
                     KLLTKSVGGNPEKLFDYYVTYGPKTIMHCKNIIMIVNGKHKAQILKQTLFGPLDLLCP
                     SSLLRLHPNFTLIVDEEAASEIKDLI"
     gene            5066..6265
                     /locus_tag="MJF80_003380"
     CDS             5066..6265
                     /locus_tag="MJF80_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBZ0282010.1"
                     /translation="MYNHMKKVFQGLEGGLFSSVEKADVGDSYQKLGEQGVSLMGWAD
                     PFFPDYCIPRHILDAATLAISSNMSTHYTAPVGNDLLKIEIAKKLKEVNNLDVDFKRN
                     ILITPGSDSGLYYSMLPFINEGDEVLIPTPCYPNNIQNTKLMGGVPIFVELKESDGYQ
                     IDITEFEKRLTNKTKLVVLTHPNNPTTTIFNKESLEKLAKFIIDNDLILICDQAFEDF
                     TFENELITPASLEGMFERTVTIFSTSKGMGLSGFRVAYMVCSDKLMDKYYGAAVTVLG
                     ATNTVAQMSTIEAFKDRSFMEDFERVFDKRRQEAYKIINEIPNVSMQMPESGFLGWIN
                     VSKLGTSSEIQKYLVDEAKVCINDGINYGPGGEGHLRIILGSYKDDSKVIGALNRIKD
                     ALIKKQQ"
     gene            6289..6771
                     /locus_tag="MJF80_003381"
     CDS             6289..6771
                     /locus_tag="MJF80_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBZ0282011.1"
                     /translation="MLSIFKSKKNDNKLKAFASGKVIPIEDVDDNVFSSKMLGDGIAI
                     YPTNEYVVAPCDAEVNIIMKDTKHAVGIKLFNGMEILFHVGIDTVSMNGEGFEIFVSP
                     GDKIKRGDKLISFNSKLIKEEGLQDVVIMVVTNSEQYKNIKYHIGLEAVIDDTIIAEI
                     "
     gene            complement(7089..7686)
                     /locus_tag="MJF80_003382"
                     /pseudo
     CDS             complement(7089..7686)
                     /locus_tag="MJF80_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7930..8358)
                     /locus_tag="MJF80_003383"
     CDS             complement(7930..8358)
                     /locus_tag="MJF80_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282012.1"
                     /translation="MKIHSIQNLTLDTRNLIKKYSLTLNEDLIWEFKHGKYHTIKYFT
                     HKFAVKESVLALLCNIHKLCYAKIKYFESSFSKYEPYKYSYQNEFVKCEMYDMNFMFH
                     KYSNIMIDLRNLQEIKTIEEFRLFCDYLESFEDNLQDLII"
BASE COUNT         3092 a          973 c         1394 g         2933 t
ORIGIN      
        1 tttgatttta agatttattg agaaaacaaa actaaatagg ggggtaatta atataatgga
       61 ttttttcaat ggtattttaa cgagtattaa caatgcagta gtatttgtaa acaattactt
      121 atggtcgtat atcttaatta taatgttagt tgtaattggt atttacttta ctattaaaac
      181 aaattttgta caattcagat attttataga aatgtttaga cttctgggtg atggtacagc
      241 gaataaaaat gcaaaaaagg aaggaaaaat ctcttcattt caagcatttt gtataagtac
      301 atcttcaaga gttggaactg gaaatatagc tggaatagca attgcagtgg tagcaggagg
      361 acctggtgct gtattttgga tgtggcttat agcattaatt ggttctgcat caagctttgt
      421 tgagagtaca ttagctcaga tttacaaagt gaaagatggt caagctttta gaggtggacc
      481 agcgtattat atggaacaag gtctaaataa aaaatggatg ggtattttat tctcaatact
      541 tataactata tgttatggtt ttgtatttaa tgcagttcaa gcaaatactg tttcacttgc
      601 atttaataat gcatttggaa taagtaaaat ggctatggga ataggtcttg gaattttaac
      661 tgcaatagtt atttttggtg gagtgcatag agttgcaaaa gtttctgaaa taatagttcc
      721 tgtatttgca ggattatata tattagttgc tttggctata gttgtaatga atataacaga
      781 gataccaagt gttatagcat tgatagttga aagtgcattt gattttaaag gaatggcgat
      841 tggtacattt atgggagttg taatgactgg agtgaagaga ggtctatttt ctaatgaagc
      901 aggtatgggt agtgctccta atgctgctgc aacagcacat gtaactcatc ctgtaaaaca
      961 aggtttaatt caatctttag gtgtttttac agatactata ataatatgta gttgtacagc
     1021 attcatggta ttattatatt caggttatag tagtagtgga gcaactggaa ttgaattagc
     1081 tcaagaagct ttaactcatc atataggacc agttggaaat atatttatag cagtttgtat
     1141 attcttgttt gcatttagtt ctatagttgg taattactat tatggtgaat caaatatgga
     1201 gtttatgagt ggaagtaaga ctaaacttaa tgtatttaga gtttttgttg taggtatggt
     1261 tttatttggt tcacttactc aagtagatgt agtttggaat cttgcagact tatttatggc
     1321 attgatggcg ataataaact taatagcaat agcactttta ggaaaatatg catttattgc
     1381 tttaaaagat tatacaagtc aaaaaaaatc aggaataaaa gaccctgtgt ttattgcaga
     1441 tgaaatagaa ggcctagaaa aggtatcaga gtggcatagg gaattatctg aggaacattt
     1501 aggataaaaa atagtataaa attataaaac cattttgaaa gactagagta tgtatatact
     1561 ttatatgaat ataatattga attaatttat attactggaa tttttaattc cagtaatatt
     1621 tttttaatta caaggatatt atgatgactt aaagactgtg gaaaactctt ttttagtttt
     1681 cattattaat attaattgac aaatttagtt tttatttaaa tttcttaagt gcatataaat
     1741 cgtatttttt gataatccca ttaagtctgc aactttaaca acagaatcct taattttaaa
     1801 aataccactt tcatttaaaa tagaaacaat agatttattt ttatcattgc tagatatatt
     1861 agaatcattt tcaacaatta cctttgcatc ttggtatgat tttaaaatta cttcatcagt
     1921 atcttctacg atattttcta aaacaggagc actttgcata gcattatgtt ccggaatata
     1981 tgaaccaatt attcttgtta gtggtgtatt aagataaaaa tgtatgcaga gtagaccgat
     2041 tattttatta tcaactcccc ttatcactat atctgttgat tttaaaggtt cacctagttt
     2101 gtttctatta aaataactga taaaatctcc atcagcatca ttttcgagtt tattaatgag
     2161 tgccaaatca aaatcagtta taatatctcc ttcttttaca gtattatatt taccattagc
     2221 tattttattt attgaatagt gtactccatt aaatttatat aaaataaact cgtatccttc
     2281 tcctaaatac gcagataatc catccactag attgttataa gaatcgaaaa tatagttatc
     2341 agtcatgtta aaagtcatat gttacctcct aagaaatatc ttaatatcat attaacatat
     2401 aaaatgaatt taataaaata tatttcagtg aaataataaa tataatgaaa aaaaaactat
     2461 tgactgaaaa atatttcagt gatattatta aaatacagta aggcaaacgt attattgaca
     2521 gaatatataa aatgttaaaa atagtatcta ggaattaaac aaattaaagg actagtgatt
     2581 tgaatttttt tagtataata tgtgacttcc aattaatatt tattaaacac caataaaatt
     2641 gtaaatatgc ttaatgatat actttagcga tgaagtgaaa atattttatt tttatgatta
     2701 acctatagga attaatttag catactgttt agctagtaaa tctaaataaa ttttagcttt
     2761 agctgatatc atgttgaata aagtttctat tggattttgt ataacaaagg agtggggaga
     2821 tggcacaaga taaaattgtg gttgaaattt tagaaaacat tggtggtaag gaaaatatta
     2881 aggaagtagc acattgtatg actagactga gaataaaggt atgtgatagt tctcaggtaa
     2941 atcaggaaaa actaaagaaa ataaaaggtg tactaggtct tgtattaaaa ggtgagcaat
     3001 atcaaattgt tataggtcct aatgtatcac aagtgtataa tactcttaat gaagttatag
     3061 gaattatacc tgagaaggat agtaaaaaaa caatatctga aggtaaacaa aatagtattg
     3121 caaaaatatt agatactatt gcaggtattt ttaatcctat agttccagca ttagcgggtg
     3181 caggtctaat aaaagctata ttagcattac tagtagcatc aaagttttta tctgcagata
     3241 gtgaaaccta tataatatta aatacaatat ctgatggtgt attttcattt ttaccattta
     3301 tgttagctta ctcagcagct aaaatattta aaatgaatac ttatgttgct gtagcaatag
     3361 caggagcaat gctacatcct aatttcacta atttaattca acaaggctta actaaaatat
     3421 cttttatcgg tattccgatg tcactaatcc agtataacgg aagtgtattt ccgatagtac
     3481 taactatttg gtttgcatcg tatatagaaa aaggagtaga taaaataaca ccaaaggcac
     3541 ttaagataat attagtacct gcattgacta ttttatttgc agctcctata gcattaatta
     3601 ttattggacc aatagctcaa atgctaggaa actatatagc tggtggagca agtatcctat
     3661 ttgaaagagg aggaatattt gcagggttaa tttacggagg aatttattca ttcatggtag
     3721 taacaggatt acatcatggt atggttcctg tattagtaga tggaataaca agatatggat
     3781 ttaactatat aagtcctgtg tcaggtagtt caaatatggc tcaagcagga tctgcatttg
     3841 gagtatggtt aaaagcaaaa gataaagatt tgaaggctgt agcagctaca gctaccatat
     3901 cagcattaac tggagttacc gaacctgcaa tatatggtgt taatttaaga ttaaagaaac
     3961 cgtttttatg cgctgctata ggtggtgcag caggaggtgg atttgctgca tatttccaat
     4021 gtaaagcatt tgctatggga ggtccatcgt ttttaactct tcctatgttt ataggagaag
     4081 gtggacatgt aggattggta gcaatagcat ttacaatagc atttatagta tctgcaatag
     4141 ctacctatat agtaggattt gaagaagagg taacagcaga ttcacacgct aacttataat
     4201 aaagagaaat tatttaacat atcaaaaagg agattaatta ctatgaaaat ttatttaact
     4261 aaagattatg aagaaatgag ccaagtggca gcacaaatgg tgttaaagta tatgtacagc
     4321 aatgataata aaagaataaa cctttctata acagcaggtc aaacaccaaa aagaacatac
     4381 gaaatattaa gtgaatttgt taagaataaa ccttattttg aaaatgtaca ttattataac
     4441 tttgatgaaa taccaataaa aggcgaaaaa gttggagtta ctataagaaa tttaactaat
     4501 atgtacttta gagaagctca aataccagaa gaaagaatac atgtgttaga tgaaaataat
     4561 tatcaaggct ttgatagaaa gatagaagcc gatggtggaa tagacatggt attaatggga
     4621 ttaggttggg atggacactt ctgtggaaat cttagtggta cagtcaactc ttttgatgaa
     4681 gagacaagag ctgtttcaac ttatataaca gatgatatgg ctaaactatt aacaaaatct
     4741 gtaggtggaa atccagaaaa attatttgat tactatgtga catatggacc taaaacaata
     4801 atgcattgta aaaatataat aatgatagta aacggaaaac acaaagctca aatattaaag
     4861 caaacactat ttggaccatt agatttatta tgtccatcat ctttattgag attacatcct
     4921 aacttcacat taatagtaga tgaagaagca gcatctgaaa taaaagattt aatataaata
     4981 agtaaggaat taaaattaaa aatgaattta ggtgcaaagt aatattttat tttgcacctt
     5041 tgctttaata aaaaggagaa taaaaatgta taatcatatg aaaaaagtat tccaaggatt
     5101 agagggtggg ttattttcaa gtgtagagaa agctgatgta ggcgattctt atcaaaaatt
     5161 aggtgagcag ggtgtaagtt taatgggatg ggcagatcca tttttcccag attactgtat
     5221 acctagacat atattagatg cagctacatt agcgatatca agtaatatgt caactcatta
     5281 tacagcgcct gtaggtaatg acttattaaa aattgagata gcaaaaaaat taaaagaagt
     5341 aaataattta gatgttgatt ttaaaagaaa tatattaata actcctgggt cagactcagg
     5401 gctttattac tcaatgttac cgtttataaa tgaaggggat gaggttctta taccaacacc
     5461 atgctatcca aataatatac aaaatactaa attaatggga ggagttccta tatttgtaga
     5521 gttaaaggaa tcagatggat atcaaataga tataacagaa tttgagaaga gattaactaa
     5581 taagacgaag ttagtagttc ttactcatcc taataatcca acaactacaa tatttaataa
     5641 agaatcttta gaaaaattag ctaaatttat tatagacaat gatttaatat taatatgtga
     5701 tcaagcattt gaagatttta cttttgaaaa tgaattaata acaccagctt cattagaagg
     5761 tatgtttgaa agaacagtaa caatattttc aacatcaaaa ggtatgggat taagtggatt
     5821 tagagttgca tatatggtat gcagcgataa attaatggac aaatactatg gagcagcagt
     5881 gacagtatta ggtgctacta atacagtagc tcaaatgtca acgattgaag catttaaaga
     5941 tagaagcttt atggaggatt ttgaaagagt ttttgataaa agaaggcaag aagcatataa
     6001 gataatcaat gaaataccta atgtatctat gcagatgcct gagtccggat ttttaggttg
     6061 gataaatgta tcaaagcttg gaacatcatc agaaatacaa aagtatctag ttgacgaggc
     6121 taaagtttgt attaatgatg gaattaatta tggtccagga ggagaagggc atttgagaat
     6181 aattttagga agctataaag acgatagtaa ggtaattggt gcattaaata gaataaaaga
     6241 tgctttaatt aaaaagcaac aataaataaa aaataattgg agtggagtat gttatcaata
     6301 tttaagagta aaaaaaatga caacaagctt aaggcatttg catcagggaa agtaattcct
     6361 atagaagatg ttgatgataa tgttttttca tctaaaatgt taggtgatgg aatcgctatt
     6421 tatccaacta atgaatatgt tgtagcccct tgtgatgcag aagtaaatat aataatgaaa
     6481 gatactaaac atgcagttgg aattaaatta tttaatggca tggaaatctt atttcatgtt
     6541 ggaatagata ctgtatctat gaatggagaa ggttttgaga tatttgtttc accaggtgac
     6601 aagataaaaa gaggtgataa attaatatct tttaatagta aattaataaa agaagaaggg
     6661 ttacaagatg tagttataat ggtagttact aattcagaac agtataaaaa tataaaatat
     6721 catattggac tggaggcagt aatcgatgat actattattg cagaaattta aaaacagtta
     6781 tttaagtaaa tacttagata atttagatat agttaaaatt aaatccttaa aatcaaatta
     6841 tttagttttt gtgtaatttt aactataaag ccgattaata atataaagta tactaaataa
     6901 tatggttatt atgattgtta ctgacaaagc ctaagtagat ataacaattt gctctaattg
     6961 agtattgcta tatctacttg tttttatatt taaaataaaa gagtctgcat tgaaaagata
     7021 attctgatga gagtaaatag ggtatttaat aatagtttaa ttctgactta tcaagatgaa
     7081 cctattcata tacctaaaac atctttaaca ccttctaaat tcatacaaat agctgtatag
     7141 gctgccttat tgattatttt tccgtcaatt tttactttgt agtgaacagc atatataaat
     7201 attatcgaat agattgtatc aagagctcta ttttgctatt cattagctaa agatagtact
     7261 ctgtcagtta tttttgaaac taatgcatga gatatatcaa ttccgtaaat attatttaaa
     7321 tgtatgatat atatcgcgag tcgacattcc ttttgagtac atgccaatta cttggttctc
     7381 tattccagaa atttcatttt cgtaattttg aataacccta ggctggaaat catccctagg
     7441 gatatttaat tttacctctc caaaatctga tttaacattc ttagctctat atccatttct
     7501 tgaatttgat gttgctttac tttggttatc atatctctcg tacattaaat gatcttcaat
     7561 ctctggctct aacattttgt gaattgtgct tgcgaataaa tcttttaaca tctcttggat
     7621 gtctttagta ttttttacat catactcttg aactaagtgt tttaatattt aatttttgtt
     7681 gtacataaaa aatgtgtacc tcctattttg gtaattagat ttaatatctt gacttttagg
     7741 agaatacaca ttttatttta cagaccctct tttctagaaa agtttttatg aaaattgtga
     7801 tataaattat taataaaaaa taattttata tttattttat tatgtaattt agttttatta
     7861 aaaatttttt attattggat atagattatc tattataaat tttatcttta agtttattct
     7921 cctaaaatac tatataatta aatcctgtaa attatcttca aagctttcta ggtaatcaca
     7981 aaataatcta aattcttcta tggttttaat ttcttgtaaa tttctcaaat ctatcattat
     8041 attactatat ttatgaaaca taaaattcat atcatacatt tcacatttaa caaattcatt
     8101 ttgataacta tatttataag gttcgtattt tgaaaaactc gactcaaaat attttatttt
     8161 agcataacat aatttgtgta tgttgcacaa aagtgcaaga acagactcct taactgcaaa
     8221 cttatgtgta aagtatttta tcgtgtggta tttaccatgt ttaaactccc aaattaaatc
     8281 ctcatttaaa gtaagagaat atttttttat aaggttacgc gtatctagtg ttaaattttg
     8341 tattgaatgt attttcataa tttattctcc tcataaatat ttaataagta tt
//
LOCUS       DAGSWI010000159         8386 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.161, whole genome shotgun sequence.
ACCESSION   DAGSWI010000159 DAGSWI010000000
VERSION     DAGSWI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.161"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            1..1064
                     /locus_tag="MJF80_003384"
                     /pseudo
     CDS             1..1064
                     /locus_tag="MJF80_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            1305..1994
                     /locus_tag="MJF80_003385"
     CDS             1305..1994
                     /locus_tag="MJF80_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282013.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            2215..4587
                     /locus_tag="MJF80_003386"
     CDS             2215..4587
                     /locus_tag="MJF80_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBZ0282014.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            4602..5504
                     /locus_tag="MJF80_003387"
     CDS             4602..5504
                     /locus_tag="MJF80_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBZ0282015.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRTQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            5524..6075
                     /locus_tag="MJF80_003388"
     CDS             5524..6075
                     /locus_tag="MJF80_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0282016.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            6239..7111
                     /locus_tag="MJF80_003389"
     CDS             6239..7111
                     /locus_tag="MJF80_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBZ0282017.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            7275..8255
                     /locus_tag="MJF80_003390"
     CDS             7275..8255
                     /locus_tag="MJF80_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282018.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
BASE COUNT         3408 a          796 c         1490 g         2692 t
ORIGIN      
        1 cttggatgct gttatatata gaatactatt ttttgcaatt acaggtaatg ttatttcatc
       61 taaagaatcc ttattagtac ctcacttaga atctataaaa attcagatat atagtaattt
      121 aataattatt cttatatatc ttatatttag cttttttagg aagtctataa aattaattag
      181 ccaagataag agaaattatg tatatttatt atttgcctta ttagttaata caatgaatat
      241 gcttgtaaat tatgtattaa aaaaattaga acggtttggg tcattacatt cagaaggata
      301 cttttgataa ttttgttaat ccaaaattgg taggagctag tagtatattt cttatactat
      361 tgtttaaaga aataataaaa gagaatagat taaaatctca agctgaatta ataaaaaata
      421 agttagatat gcaatatgcg cattatttaa gtattcaaga atctcatatg cgagttaaga
      481 aactatatca cgatataaat aatcacattt attgtataga caaccttaga aacaatagca
      541 aagaaattaa tgaatatgtt aacaatttaa aagatgaaat aaaaacattt aaatatatct
      601 ataatacagg aaatatgata ttagacatta taatcaacga aaagagtgaa gtatgtttaa
      661 aaaaaggaat taaatttact tgctctataa atttttcaaa agtaaatttt gtaaaaccta
      721 tagatgtgtc aagcatattt tctaatattt tggataatgc aatagaagca tgtgataaaa
      781 tagtggatga aaatattaat aaatatatta gaattaaagg aactataaca aggtcatttt
      841 ttgtacttaa atgtgaaaat agtaaactaa atcagcttac ttttaataaa aatatattgt
      901 taacaaacaa aatggataag ttcgtacatg gaataggtat tcagagtatt aaatcttctt
      961 tacaaaaata taatggagaa ttattatttg aaaatagtat cgataaattt atactaaata
     1021 tatatatacc attggaccaa gatactgaca gttggggcga ttaagttacc agttgggaca
     1081 tatgtattga tacaaaaaaa taaggatgtt atcttttaat aacaaaagat aacatcctta
     1141 tttctgtatt aaggagctta taattttact aatagtaagt tataagaatt ggataggaag
     1201 gtgatatgta ttgaataata aatctttaat ataaagaaaa tataaatata ttctagtgca
     1261 atgaataata ctttagagcg gttagtgtga tgggaggatt gtgtatgaaa gtaaaaagga
     1321 atataaatag agtggcaata attgtggctt taaatcttag cttagcattg atggtaggat
     1381 gtatgtctcc tgtatctgat aagaacttaa gtagctcgga acaaattagt aaagattcat
     1441 tgaaagaaca agttgaagat aaaaaagatg aatctatcga gctatttaat aattactcac
     1501 aaaagattaa tgatgcgaaa ggtagagaag aagtaaaaaa agatttatct caaagatttt
     1561 cgaaagctat aatagacagt ggttatagta ttactaactc aaaatcagat ggtagctttg
     1621 tagtatctat aaatggtgct gaaaaaccac ctgaaaaaaa tattcgaagt ttattctatt
     1681 ctatgtctga agacgataaa gaaggaaaag taacaataga tattctctgt gctaaagagt
     1741 atccagaaga tgataagtta tctgagagtg ataaatatat taaatttata tacaatttat
     1801 ttaaatcatt gactaatacg aacttaactg agaaagaaat tttttctatg gttgaagaag
     1861 actttaataa gggagtaggt gttgtagagc ttccttatat gaaggatata catgttgaag
     1921 ttaacaaagt taaacaatca actaaggttt taaaactaag tctagtatac aaatttgata
     1981 tttcaaatca ttagtatatt agaaataact ataacaatat agaaaatata tcatagaaaa
     2041 tttttttaga ttttataaat ttaacaaaaa aagtattaat atataggtgt tcaaggtaga
     2101 tgaaaaaaaa taagttattt tgcttccatt ttatgctaaa ggacagtata atataattaa
     2161 agtatgttat aacatacaaa aatgtagtat atattatcca aagggggatt ttaaatgagt
     2221 caaactaata gaatagaagc atttagacaa gaatatatta attctaaacc tatgatatgt
     2281 tgtgaaaggg ctagaatttt tacagaatca cataaaaaaa ctgagggaga agctatctgt
     2341 ataagaagag ctaaagcttt tttggagaca tgtaaagaac ttcctataaa aatctttgaa
     2401 aatgaattaa tagtaggtac agcaggaaaa tttagaagaa cagggatatt gacaccagag
     2461 ttttcttggc aatgggtaga caaagaaatg gatacttttg ataaaagaac tcaagaccca
     2521 tatgtaatat caaaagaaca aatagaattt ataagaaaag aaatatttcc atattggaag
     2581 ggaaaatctt tggaagaagt tttcttagca agaatcccag aagatacagc taagatatta
     2641 gtagatacag gaataataga taatgattct aagtggagac aggcagttgg tgaagtaaca
     2701 ccagattatc aagatatatt gtttgtaaaa ggatataaag ggattaagga agatgcagat
     2761 aaaaaaatta aagaattaga tatatcagtt tcagaaaata ttgaaaagat agatttttac
     2821 aaatctgttt ctatagttgc acagggtatt atgacattgg cacaaagata ttcaaatctt
     2881 gcaaaagaga tgagtaagca agaaacagat gaaaagagaa agttagaact tataaaaatt
     2941 tcagagatat gtatgaatgt accagcaaac cctccaacta atttttatga agcaatacaa
     3001 tttgtatggt ttgttcaatt aggtggtatt ctatcagaaa atccattggc attaaattta
     3061 ggtagatttg accaatatat gtatccatat tatgaaaatg atgctagaga aggtaaaata
     3121 actgaatcag aagctcaaga attgatagaa gcactttgga ttaagttatc agaatgggta
     3181 tggactatat cagcaaatac agcaaattac tttgcaggat ataatcaatt ccaaaatctt
     3241 actgttggtg gtaaaaagag aaatggtact gatggaacga atgatatctc atatatgtgt
     3301 ttaaaggcta cagaaagtgt aaaaactcac cagccaggat taagtgttag agtgagtcaa
     3361 ggagcaccag ataatttcgt aatggcagtt gctaaattgg taaaacaagg aactggtttc
     3421 cctgctatac acagcgatag tgcaggggca caaatgttat tacaagatgg atatgatgca
     3481 gaagatgcta gagattggag taactgtggt tgtgtagttc ctcattttag aaaaacagga
     3541 cagtggactt cagccgtaaa tatcaatttt gcagcagctt tagaatatgc tatgaatgaa
     3601 ggtaagagta gattaactgg agaaaaaatg gggttagata caaaaaatat cacagaattt
     3661 acttcttttg aagaacttaa ggatgagttt ttaaagcaat tagcatatct tgtaaaaagt
     3721 tctgttatag gaactactgt tgctcaacaa attcataaag aaatggtacc aagaccattt
     3781 ttatctactt gtgtagatgg atgtttggat aagggagttg atttaagtaa aggaggagca
     3841 aaatataata taggtcctgt attaactggt atagggttag gtgtagtttc aaattcattg
     3901 gcagctataa aaaaattagt atttgaggat aaggtgacta cgttagaaga attaacaaaa
     3961 gcactaaata atgattggga aggttatgaa gaattaagaa aacttgcgtt ggatgttcct
     4021 aagtatggaa atgataatga ttatgtagat tcattagcaa ttgaagtttc tgatttttat
     4081 tatactgaaa ctagaaaata caaggatatt tttggctcta aatttaatag tgcatttatg
     4141 ggtatatcaa actatgtacc aactggaaaa atagtaggtg caactccttg tggaagaaaa
     4201 gcgacaaaac ctttgacaga gggagtttct ccatttgttg gtactgatac aacaagtcca
     4261 ctagcagcaa tgaaatctgc atcaaaaata aatcatgatg ttcatactgg tggaacactt
     4321 ctaaatttaa gactgaatca agatttggtg gaaactgaaa gaggacttag aaatctaaca
     4381 tctatgatta aatcatattt tgctttagga ggattccatg tacaatttaa tacgatatca
     4441 aatgatactc tattaaaagc tcaagaaaat ccagaagaat ataaagattt attagtgagg
     4501 gttgctggat atagtactca atttgttaat ttatcaagag aaatgcaaga tgctataata
     4561 gctagaaatt cacatagcaa tttttaatta tgaggtagat tatgagtaaa aaaggtagag
     4621 ttgtaaaagt acaacatttt tcagtaaatg atggagatgg aataaggact actatatttt
     4681 tagaaggatg taaattaaaa tgtaagtggt gttctaatcc agattcctgg agtaacatag
     4741 taaaattagg tgttatgaaa gataaatgtg tatcctgtaa tagatgtata gatgtttgtc
     4801 ctcaaaatat aagttcacta tttgatagaa cgcaaataaa taataaatgt gatttatgtg
     4861 gagagtgtat caaggtatgt cttaaagatg ccatttgtat aatgaccgaa gaaatgagtg
     4921 ttgaagaaat agtagaggaa gttgaaaaag attttatatt tttctttgaa tctaatggag
     4981 ggataacttt ttctggaggt gaacctacac ttcagattga ttttttaaga gaattagttg
     5041 atatttttta tgataaaggc ataaatattg caattgaaac ttgtggatat tttgattgga
     5101 ataaggtaaa tgatgtattt gagaaaatag accacatatt tgtagatata aaatctatgg
     5161 atgataatat tcataaagaa tatacaggag taagtaataa aattatatta gataatatat
     5221 gtaggctttc aaagttgaat aaatctatgg ttataagagt gccaattatt tatggagtaa
     5281 atgatagtga agaaaatata agaaatactg cattatttgt taaacaaaat gttccaggag
     5341 gcaagatgga gttacttcca tatcataaat ttggaatcga taaatataag gcattagggc
     5401 ttgaagatta tatatatgaa tttgatgaaa tttgtaacaa tcatatgtta aaattaaaag
     5461 agatagtaga acttacagga gtaaaaatca tagaatacaa atagctatca ggaggatttt
     5521 aacatggata ataaatttcc taatgtggga gaaaatttaa ggttattgag acaagaaatg
     5581 ggcataagtt tagacaaagc ttcaaagatg acaggtgtca gtaaagcaat gcttggacag
     5641 attgagagag gtgaatcaag tccgacagta tctactttat ggaaaatttc atctggattt
     5701 aaaataaatt ttactacact attaaatgaa aatacaaata cctatgaggt tataaagaaa
     5761 gaagaagtag agcctattgt tgagcaaaaa gggaacatga agttatatcc aatataccct
     5821 tttagccctc aaagaaggtt tgaacttttt ataattgagc ttgaggaaaa ctgtactcat
     5881 gtttcatcta cacatagtca tgttttagaa gaatatgtgc tcgtaataga aggtaaactt
     5941 gaccttaatg tggatgggaa aacttatatt ttagagtctg gacattctat taggtttgat
     6001 ggaactttag agcatatata taaaaattta aataaaggaa aaactatatt tcacaatata
     6061 gaggtttata gataatttta tagttatcct tttctaaaat aaatgaaaat tttagaaaag
     6121 gataacttat ttttataatg tcgaaaagat ttatatgtat gtgatgatgt tattgtttta
     6181 aaaaaaacat aaaagcatta taataatatt aacaaaatag aataaagagg aagagaatat
     6241 gattataaag gtttcaatga cagtattagt attaatatta ttgtatattc tgtataagta
     6301 tgaaactcag catatagaaa cgacagaata tactattttc aacaaaaaaa ttccaaagga
     6361 atttgataac tttaaaatag ttcaagtgag tgatttacat aataaggtgt ttgataaaaa
     6421 taataaggta ttgatagata aaattgaatc attaaaccca gatgttatat ttataacagg
     6481 agatttagta gatggagaaa ataaaaattt tcaagttgca ttagatttaa tagataattt
     6541 ggttgaaaaa tatgaagtat atcatataat tggaaatcat gaacaaaaat ctttaataaa
     6601 aaagtataaa catctatata aggattattt taaggaacta tattcaaaga aaataataaa
     6661 tatggaaaat gaatctgtaa gaatatatag aggtaatagc catcttaatt tatatggttt
     6721 aattatacca ttggaatgct atccatattt atttgctaat aacaaaaaat tagaactaag
     6781 tgacaatttt gtagaagaca atctaggtaa agttaatgaa aatgaatata acattctttt
     6841 agcacataca ccatttttct ttgaaaaata tgcaaaatgg ggagcagatt tggttttagc
     6901 aggacatgtt catggaggta taataagaat accttttgtt ggaggtgtat tatctccaaa
     6961 tagagagttc tttcctaagt atgactgggg aaaatatgaa aaagataata gcactatgat
     7021 tttaaataaa ggcttaggag gctcaaaagt tttaattagg ctcaattgta aaccagagat
     7081 agttaagatt acattgaagt atgagaaata agattttata taacatatat aaacatcaat
     7141 aacatataaa taaggttgat gttaaatcca tttaaacata tataatgacc aattttgaat
     7201 aattgttgaa ttaataaaaa taaagtgcta tttttagcat agaatattta aactatgggg
     7261 ggtagtaaag aatcatgggt gatacaagta gattttatga gtctgtaaaa aataaatatc
     7321 cgaaattaaa agatggaact agggttcatc tgtggccaaa agaaaacatt ataattggcc
     7381 ctatttatga tgaagaagat ggatataaaa aaagtatcgg aaatgtggat agcttaagtt
     7441 ggaagcaaat tgttatactg gctgaatgtg atgggaaaaa tactactgac gatataataa
     7501 atatattatc caatagatat agaaatatcc ataatatgaa agaaaaagtt atggaatttt
     7561 ttatgtttta tgagaatgta tacttaacgt ttgaagatga tatacagaca ctatcaagtg
     7621 tatttgagat aacaggaaat aaatcataca ttactccttt gtactttact atagaaatag
     7681 atggagataa taatacaaat gattattttt ctgaaatcac aagtttattg aactgcatgt
     7741 atgagaaagg ctgtagattt atagaggtta ttggtgagga tattttaaaa aataaaagta
     7801 tgagagaaat atttcaatat atgttagacc attttgattt aattgtggta actaaagata
     7861 gttttactat tgatagaagc ttaataaagg aattagataa ttatcgtcat aaagtgatat
     7921 ggaaagtata ttcaaaaaat gatgatacac gtattaaatt aaaagaagat ataaaaataa
     7981 ccagtttaat aaaaaggggt cacactgtag taagaggaga cagagaaaaa gcaataaaaa
     8041 tagaagatat gaggtttaaa gatacaaagg agaggttttc aaaatatgga gcagagtgga
     8101 gtcatttata tatttcttct gatggtagtg taaaatctta ttcatttcaa aaagataaaa
     8161 gaatctattt aggcaatata tttaacaatt ctgttgaaga aattttctta gaaatgcaaa
     8221 ataaaattga caaactagaa attgtgtatc aataggggga aagtttaaga tttatagaag
     8281 atttatagat tgtttattta agtttgtgtc aataattata atgaggtgag attacttgtt
     8341 aaaattattg gacaaatttt tttatgatgt tttacttaaa ttttgg
//
LOCUS       DAGSWI010000160         8365 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.162, whole genome shotgun sequence.
ACCESSION   DAGSWI010000160 DAGSWI010000000
VERSION     DAGSWI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.162"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            134..547
                     /locus_tag="MJF80_003391"
     CDS             134..547
                     /locus_tag="MJF80_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBZ0282019.1"
                     /translation="MIELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            749..1390
                     /locus_tag="MJF80_003392"
     CDS             749..1390
                     /locus_tag="MJF80_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBZ0282020.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1414..2667
                     /locus_tag="MJF80_003393"
     CDS             1414..2667
                     /locus_tag="MJF80_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBZ0282021.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            2713..3363
                     /locus_tag="MJF80_003394"
     CDS             2713..3363
                     /locus_tag="MJF80_003394"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBZ0282022.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKSSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3414..4010
                     /locus_tag="MJF80_003395"
     CDS             3414..4010
                     /locus_tag="MJF80_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBZ0282023.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4023..4862
                     /locus_tag="MJF80_003396"
     CDS             4023..4862
                     /locus_tag="MJF80_003396"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBZ0282024.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5107..6261
                     /locus_tag="MJF80_003397"
     CDS             5107..6261
                     /locus_tag="MJF80_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBZ0282025.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNSKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            6567..8264
                     /gene="ade"
                     /locus_tag="MJF80_003398"
     CDS             6567..8264
                     /gene="ade"
                     /locus_tag="MJF80_003398"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBZ0282026.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKLYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3414 a          819 c         1496 g         2636 t
ORIGIN      
        1 ttatacagcc tctttattta ttgattgtat tattaataaa ttttaaatta tgataaaata
       61 taaaacaagt aacttatttc agaataaaat actttttaaa agaaagggac aagatatggg
      121 agattataat aaaatgattg aattaaagaa taacttaggt atcagtgaaa gaaagttaaa
      181 atatccttgt atctataaac attttaaagg gaaatattat gctacaatgg gtcttagcaa
      241 ggctattgat gatatagaaa acatatgtga aatatatggt aaagaaaatt taatccaaaa
      301 cagaaataaa tataaattag ttattagaca tactgaaaga gaagaagata tctatgttta
      361 tagggattta gatggaaact tttatcataa aaaagaagaa gatactaatg atttagtttt
      421 atacaagacc ttatatgatg atacaggaat atttgcaaga cctttggata tgtttttaga
      481 aaaagtagat acagataagt atgtcaattc aattcaagaa tacagatttg aagaagtata
      541 taaataatat aagagaaata tgcttataaa tttagaaaaa ttttttaaca gtacaataat
      601 ttgcagaaat acaaatgaga tttattgtaa ataatatgtt agattagatt ataaaataga
      661 aacaaagtaa agtattatat gatataatta agttttgaga agtgataatt tatatttaat
      721 aaaaaatagg catatattag gaggacatat gggaaatact ttttatatag taagacatgg
      781 acaaacggat tggaatatat tgggaaaaac acaaggtcat ggtaattctg accttacgcc
      841 tcaaggtata gaacaggcaa aagaactttc tgaagatatt ggcaagtatt caattgatta
      901 tatttttagt agcgaccttg ggagagcaat gcaaacagct caaattttag gagataagct
      961 caatattgaa gttcaaaaaa ctgaggcact tagagaaatg ggattcggag tttgggaagg
     1021 ccttttaata aaagaaattc aaaaagatta ttcagatata tatgcaacat ggagaaatga
     1081 accacactta gtaaatatac cagaaggcga aactcttaag attataaagg aaagagtaga
     1141 tgcttttata aaggaactaa atgaaaaata tgataataaa aatataattc ttgttacaca
     1201 ttcaattaca ttgagggtta tgttgttgtc gtttttagaa tcaggtatgg aaaatatata
     1261 tagaattaag caagataata ctgctttaaa tatagtagaa tttaaagatt atggacctgt
     1321 tatagtaaaa atgaatgata caagtcatat aaaaaatcat gtaaagataa ataattctgc
     1381 actggagtag gaatcaatta taaggagaaa tttatgagta aagttatagt agttggtgga
     1441 ggagcttctg gaatgatggc agctttgtca gcttcaaaaa acaataatga agtaatatta
     1501 gttgagagaa atggagaatt aggaagaaaa cttagggcaa ctggtggcgg tagatgcaat
     1561 tttaccaata atagagagat agaagatttt tttgataaag ttgtcagtaa taaaaaattt
     1621 ctctatagta gtttctatac atttacaaat aaagacctta tttcgtattt tgaaagtaga
     1681 aatttggaat ataagattga agaagaaaat gaccacaaag tatacactaa aaatgataaa
     1741 tctatagaag taatagaagt attaaataaa gatttactca atcacaatgt aaagataatg
     1801 tataataaga aagttataga tattataact gaagaaattg ctctaaaaga tgattctaat
     1861 aaggacaagt caaagtatct aataaaaggg attattttag ataatggaga taagatacta
     1921 ggagacaagg ttattatatc aactggagga gtgagttatt caaagacggg ttcagatggt
     1981 tcaatgtata agattcttaa aaagcatgga catacattaa acaaactata tcctgcttta
     2041 gttccactta caatagagga aaaatggatt aaagatttac aaggaatatc catgaagaat
     2101 gtggaaatat cttgtaagat taagaaaagg aaaatatcaa aatctggaga catgctattt
     2161 gcccattttg ggataacagg gccttgtgtt cttataatgt cttcttatat taacaagatt
     2221 attgaaaagg aaaaggtaga attaaatata gatttcttac caaatttaag tactgatgaa
     2281 atctctagta taatcagagc atttccaaat aaaaatgttc taaataatct taagcaaata
     2341 ctacctcaaa atttcttaaa agagattttt tctctattat ctttagttga taaaaaggca
     2401 agtgatttgt ctaaggcaga tgaaattaga attattgagt atataaaaaa tatgaagcta
     2461 acttgtaatg ggactacagg tataaacact ggtatggtta cttctggtgg tatttcagtt
     2521 aaagaaatta attcttctac tatggaatct aaattagtta agaacttatt ttttacaggt
     2581 gaagtaattg atatagatgc agaaactggt ggatataatt tacaaatagc attttcaact
     2641 ggatatcttg cgggtataag tgtataaata taatttttta ataattaaat atgaataaat
     2701 agaggtgtat atatgggtaa tttagttata gctgtagatg gtccagcagg agctggaaaa
     2761 agtacaatag ccaagatagt agcaaaaaag ctaaatataa attatataga tacaggtgct
     2821 atgtacagag cagttactta taagtgttta aaaagtggta ttgatgtcaa taatgaaaaa
     2881 gaagttattc aaatagctga aaattcggat atagatttta aagataacaa tatatatttg
     2941 gacaaagaag ttataaatga agaaataaga acaatagaag taagcaataa tgtttctaat
     3001 gtagcaaaaa taaaagaagt aagacagttg atggttgaag ttcaacgaaa aataggaatg
     3061 aaaagttcag taatactaga tggtagagat ataggttcat atgttttccc agatgcagat
     3121 tacaaattct ttttagtagc tactccagaa gaaagaggaa atagacgcta taaagaactt
     3181 tgtaataaag gatataatac tactttagaa gaagtaattg aggatataat aagacgagat
     3241 gaaattgatt caaatagaga atttgcacca ttggtaaaag ctaatgatgc actagaaata
     3301 gatactacag gaaaaactat agaagaagtt gtagaagaag ttgtatctaa aataaatctt
     3361 tagtttttta tgcatcataa tgaaaataaa tgtagtgttg aagggagata aacgtgaatt
     3421 tttatagatt tgtaataaat atttttaagg gtttttctaa aatatttttt aaatatgaag
     3481 ttataggtgc agaaaatatt ccagatagag gaaatatagt aatagcatca aatcataaat
     3541 cgaatcttga ccctatattc ttggctgctg ccatagaaaa tagagaaata gctgcaattg
     3601 caaagaaaga attgtttaaa gtaaaaccat tgggatttat attaaaaaaa ttgcatgtta
     3661 tgcctataaa tagagaaaaa ccagatgtat ctacaataaa aactatttta aggtcagtta
     3721 gagatggtta tgtattagga atatttccag agggtactag aataaaagga gattcttttg
     3781 gtaaggctaa ggcaggtctt tctgtattta caataaaaag taaatctaaa gttgttccag
     3841 tatcaataat atctaaatat aaacttttta gtaaagtaat tgtatatatt ggagaaccaa
     3901 tatcatttga agaacatttt aaggaaaaat tgagtaatga tgaccatgaa aggatatcac
     3961 aagagatttt agaagtgata aaacaaaatt actttaaata ttcaaaataa gggggtaaag
     4021 ttatgaacat aaaaatagca aaaaatgctg gcttttgttt tggagtaaaa agagcaatga
     4081 aaatggcatg ggatgaagtt gaaaaaaatg attctggcat atatgcttta ggtcctctta
     4141 ttcataacaa gcaagcagta gcaaaatatg aagaaaaagg tcttaagact gtgaatgaaa
     4201 ttgatacaat accaaatcat gaaaatatga taataaggtc tcatggagtt cctgaaaata
     4261 tatataagga agccaaagat aaaaaattaa aaattgtgga tactacatgt ccatttgtaa
     4321 agaaaataca tacagtagta agtgagtatc ataataaagg ctatgaaata atagttattg
     4381 gagatatgaa gcatccagaa gttataggta taaatggatg gtgtgaaaat tcagctataa
     4441 taataaagac cttagaacaa atggaaaata tggaatttga taactccaaa aaatattgtc
     4501 ttgttgcaca gactacaata aatccagagt tgtatataag tatagttaat aaactatcag
     4561 ataaattaga agaaatagta tttaatgata caatttgttc agctacaaaa acaagacaag
     4621 aatcagctaa agagttagct aaagaagttg attgtatgat agttatagga ggtaaacaca
     4681 gttcaaatac tcaaaagtta gtaaaggttt gtgaagactt agtaccaact tttgcaatag
     4741 agactaaaga tgaattggat gtaaatacgc ttaaaaaata taaaaatttg ggtattacag
     4801 caggggcatc tacacctaat tggataatag aagaagttgt aacattcctt gaaaatttat
     4861 aatttgatag gtaataaaaa taggtggaca ttgttataat ttatcataat atccaccttt
     4921 gatttcatat tacatgtggt aaatagataa cctaatatca aaaagtgtgt atcttaagat
     4981 gaaaaactaa gagtataaga tgcgcacttt ttaatattag aaattcaaaa gaaatattga
     5041 tattgattat caatagaaaa cagaatataa tataattgtt gcaaaattag tctaggaggt
     5101 aaattaatgg aaatgttgat atccgcttct gaagaggcgt ttttacatgt aggttctatg
     5161 attggatttt ttattttgtt atttggatat attaattata aaactagtgg aaattttact
     5221 aatatcatat caaaaaacag aaaatttcaa ccattaatag gtgctttaat tggagctata
     5281 ccaggttgtg gcggaagttt ggcaataatg ccattgtata taaatggaaa attaagcttt
     5341 ggagcaataa ttgcaagttt aatagctagt atgggagatg cagcatttgt acttatttca
     5401 tcaaatatta aaatgtattt ttttgtcact atagttagta caattacagg aataataact
     5461 ggacaattag tggattactt taaactagaa gaaaaattag ggttaaaaaa ccgtagaaaa
     5521 tcagataaat atgatataag taaaaataac tctaaagaaa ataaagaaca acataatcat
     5581 gatgaaataa ttttagacac tctagctaaa tctcatggaa atacaaatag acttgcgttc
     5641 ataataacac atggaaaggg atataaaatt tatataggaa ttatagtaat cggttttata
     5701 tttatgtcac ttgctcactc tggacttaat cttcctataa ttgagaagtt acattcattg
     5761 gaagaagtaa tagcagtgat tggaatatta ttttcaataa tttatatgtg gtgttttaaa
     5821 aaggtattta aaaattctaa tcaaaaagag gaagaaaata aaaagatttc tttgcgagaa
     5881 atgcttatcc attctgttgg agaaatatca tttgtaatta catggatttt tattgcatat
     5941 cttatttacg atttgataat attaatttta ggtggagatg aatacttagt taaactagta
     6001 ttatcaacag gtgtaataag tgtatttata ggtgcaggtc ttggacttat tccaggttgt
     6061 gggatacaaa ttgtgcttat gtcattttat ttgaagggaa ctataccact tggtgcagtg
     6121 atagccaact ctatttctca agatggagat gcacttttcc cacttttagc aatggataaa
     6181 aaatcttctt tatgggctat gattattact actataccag caatacttgt aggtatgata
     6241 gtttatgcat ttttaggtta aatataatta cgaataaaga agtattaaaa aaataataaa
     6301 gcacataaat ttatttataa gaatagtgaa gattaaatat aaaaataaaa aattcaatat
     6361 gaattaaatt attgcttaaa tattaatgag aacatgggag ataatattat tcctcatgtt
     6421 ctctctattt aattaaagga attatggaac aaatttcaag tgatactttc tatcaaaact
     6481 catatataat atatattaat aaattaaact ataatacgta atacagtcag tatattgata
     6541 taaaaaactt aggaggggtt ataaaaatgg gtaatttaaa aagacaatgt gatgtttcaa
     6601 gtggaagaga aaaagctgac attgtactaa aaaatgggac aatcataaat gtatttacag
     6661 aagagttaat aacaggagat gtagcaatag taggagacac tatcgttggt attggcgatt
     6721 acaagggcaa tgtagaaatt gattgtagca acaaatatat atcacctggc tttattgatg
     6781 cacatatgca tattgaatct acaatggtaa tgccaattga attatcgaaa aaattattaa
     6841 aatcaggtac gacaactatc atagccgacc cacatgagtt ggtcaatgta aaaggagcta
     6901 gtgcaataga ttttttatta gaatcgacaa aagatatacc acttaatgta tatattatgg
     6961 tgccttcatc cgtacctgca acaagttttg aaactaatgg agttggtaaa tttagtgcaa
     7021 aagacatgga gagttatgta aataatccta gaattttagg tttaggagag gtaatgtgtt
     7081 ttaatgatgt tataaattct gaaaatgaaa tcttggataa actagagctt tttaaaaata
     7141 aagttgtaga tggacatgca cctaatataa atggtaaatc acttcagaca tatgtatgtg
     7201 caggaataga aaatgaccat gaatgtataa cttttgatga agtatatgaa aaattgagag
     7261 caggattaaa aattttaata agagaaggaa gcgcagcaaa aaatttaaag tctatagtaa
     7321 gtggtatgct aaagcataac ttgccaattg aagaatttat gttttgcaca gatgataagc
     7381 acttagatga tatagaaaaa caaggtcaca taagatggaa tataaaatgt gcgattgatt
     7441 taggtatgga acctgttagg gctataaaag ttgcaactta taattctgca aaagcatatg
     7501 ggttaaggaa aattggagca ataggtgcgg gttataaggc agatattgtt gtattgaatg
     7561 atttagacaa aatggaagtg gatagtgtat ataaagatgg taatttggta aacgaagaga
     7621 tgttttctaa ttacaattat gagattaaag ataaagaatt gcttaataca gtcaagttta
     7681 aatatattaa taaagaaaaa atacagttaa aagtaagtga aaaaaattat gtaatggaga
     7741 ttgtacctta tcaaattttg acaaataagt tatatgaatc tttaccatgt gcagatggat
     7801 attttgttcc aaataaagag tattcaaaac tttgtgttgt tgagagacat agaatgactg
     7861 gaaatgtagc aattgctcca ctaaaaggat ttggcataaa aaatggagca attgctacta
     7921 ctgtggccca tgattctcat aatattatag ttgcagggga taatgatgat gatattctag
     7981 tagctataaa ttacttaaag gaaatacaag gtggttatgt aattgtatca aatggaaaag
     8041 ttcttgcaca cctaagtctt caagtagctg gattaattag tacttttaca gctgaagaag
     8101 tacaagaaat tactgataat atgttggaga tagcaagaaa aatgggagtt ccagagtatg
     8161 tagacccttt tattacactt tcttttatgg cacttccagt tgttccacaa ataagactta
     8221 cagatttagg tctatttgat gttgaggaat ttaaatttat atagataata taaaagtaat
     8281 aataaatgag gctttctcaa aatagggata agtctctttt tattgttgaa atgatggaaa
     8341 tatatttgat ttcatacaaa aaaga
//
LOCUS       DAGSWI010000161         8235 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.164, whole genome shotgun sequence.
ACCESSION   DAGSWI010000161 DAGSWI010000000
VERSION     DAGSWI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.164"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     regulatory      189..232
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            333..1301
                     /locus_tag="MJF80_003399"
     CDS             333..1301
                     /locus_tag="MJF80_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBZ0282027.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            1303..1866
                     /locus_tag="MJF80_003400"
     CDS             1303..1866
                     /locus_tag="MJF80_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBZ0282028.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPI
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            1874..2563
                     /locus_tag="MJF80_003401"
     CDS             1874..2563
                     /locus_tag="MJF80_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBZ0282029.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNYFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKDMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            2690..3418
                     /locus_tag="MJF80_003402"
     CDS             2690..3418
                     /locus_tag="MJF80_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBZ0282030.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            3432..4106
                     /locus_tag="MJF80_003403"
     CDS             3432..4106
                     /locus_tag="MJF80_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBZ0282031.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            4132..4968
                     /locus_tag="MJF80_003404"
     CDS             4132..4968
                     /locus_tag="MJF80_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282032.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            4984..5655
                     /locus_tag="MJF80_003405"
     CDS             4984..5655
                     /locus_tag="MJF80_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282033.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            5645..6877
                     /locus_tag="MJF80_003406"
     CDS             5645..6877
                     /locus_tag="MJF80_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0282034.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            7027..7344
                     /gene="trxA"
                     /locus_tag="MJF80_003407"
     CDS             7027..7344
                     /gene="trxA"
                     /locus_tag="MJF80_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBZ0282035.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            7361..>8235
                     /locus_tag="MJF80_003408"
     CDS             7361..>8235
                     /locus_tag="MJF80_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBZ0282036.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
BASE COUNT         3408 a          770 c         1465 g         2592 t
ORIGIN      
        1 atgcgccaca tcttattaaa gttggttcat aattctaaat aaactttaat aagatacatt
       61 aaaatataat aaattctttt atgctataaa atttatttta tagctattaa atcagatgtt
      121 taaaaatttt ttaacagtct atagttttta agagtctata gtaagtagcc taaaaatatg
      181 attttacatc tgtacttgtt cgagaactcc aagtataaaa aactaaggaa ttatatagtg
      241 gcagtatata attaataccc ttagacacac ctttttgaaa gtggaaaaaa gatgtgtagt
      301 tctcctcgac cacaaatcaa ggaggataaa aaatggaaaa gagaaaagta ataattgatt
      361 gtgacccagg aattgatgat tctttggcaa ttcttctggc tttaaactca ccagagctag
      421 aagtaattgg aattaccaca tgttgtggaa atgttccagc aaatataggt gcagaaaatg
      481 cactaaaaac acttcaaatg tgttcttcac taaatattcc agtatatata ggagaagaag
      541 caccactaaa aagaaaactt gtaacagctc aagatacaca tggagaagat ggtattggag
      601 aaaactttta tcaaaaggtt gtaggagcta aagcaaaaaa tggagcagtg gattttataa
      661 taaatacttt acataatcat gaaaaagtat caataatagc acttgcacca cttacaaata
      721 tagctaaagc acttattaaa gataagaaag catttgaaaa tctcgatgag tttgtgtcta
      781 tgggaggagc atttaggatt catggaaatt gctctccagt agcagagttt aattattggg
      841 tagacccaca tggagcagat tatgtttaca agaatttatc taaaaaaatc cacatggtag
      901 gtttagatgt aactagaaaa attgtactta ctcctaatat tattgagttt ataaatagac
      961 ttgataagaa gatggcaaag tatataactg aaataactag attttatatt gatttccatt
     1021 gggaacagga gggaataatt ggctgtgtga taaatgaccc tctagcagta gcatacttta
     1081 tagatagaag tatatgtaaa ggatttgaat catatgtaga agttgtagaa gatggaatag
     1141 ctatgggtca gtctatagtg gattctttca atttctataa aaaaaatcct aatgcaattg
     1201 ttctaaatga agttgatgag aagaaattta tgtacatgtt tttaaagagg ctttttaaag
     1261 gttatgaaga cattatagac tctgtggaag gagtgatata gtgtgaaaag aaaaatatgt
     1321 gtaaaaacta ttacacttat agcttttggt atttctataa atataatagg cgcttttata
     1381 gctatgggat taagattacc tgtatactta gattcaatag gaactataat gatagcttca
     1441 cttttaggac ctaaatacgc tgttgttaca ggtgtgtttg gaagtttaat aagtggaatt
     1501 acgtttgatg tgtattctct ctattttgca ccagttcaaa tatcaaccgg attacttgct
     1561 ggaatagcat ttaaaaagaa ttttttaagg ggtttaaaaa ctccaatagg agtactttta
     1621 tttgccattc caacatctat aataagttca ataatttcag catttttatt tggaggtatg
     1681 acctcttcag gttcatcata tatagtgcaa atcttgaaag tgttaggact gggagatgtt
     1741 tttagtgtgt ttgtaaccca agtatttact gattatggag ataagctgtt agcagttgtt
     1801 ttagtgaatt taggattaaa tgcagttcca aaaactttaa aggtatcatt gactggagga
     1861 aaatagatag aaaatggata gatatagtga gataacaaat aaaaatcaaa gagaaattgt
     1921 tctgcttaaa ggatttcctt gtatatgggg aaagtgttct ttttgtgatt atatagatga
     1981 taattctaat ttagaagaag agatgaataa attaaatcta aaggttttaa aaaacgtaac
     2041 tgggaaatat ggagttcttg aagtcataaa ttctggaagc tgttttgaat tacctaagga
     2101 tactttagaa aaaataaagt gtattataaa agagaaaaat ataaaaaaat tatttttaga
     2161 aagtcattgg tcttacaaaa ataggcttaa ggaaatgaga gagtattttg aaattcctgt
     2221 ggtatttaaa attggagttg aaacctttga caattatttt agaaataata ttttaaataa
     2281 aaatgcaaat tttaagactc cacaagatgt aaaagaatat tttgattcac cttgtattat
     2341 ggttggaata aaaggacaaa caaaagatat gatagataaa gatatagaga ttatcctaaa
     2401 tacttttgaa aaagcgacag tgaatgtttt tataaataat tcttctagta taaaaagaga
     2461 tgaagagtta gtgaaatggt ttgttagtaa gtataaattc ttggatgaaa atccaaatat
     2521 agaagtttta tacaataata cagactttgg tgtaggagat tagcattaat ttagtttaaa
     2581 tttaaaattg aaaagtttct tatgtttttg aaaaagcttc taatagttag aagctttttt
     2641 tgtagagttt tatgttacaa taagaaaaaa ggaattaagg agcaattaaa tgatgtttga
     2701 taggggaaaa tggaataaat tggattataa tgaactttta gaatatatga tgtctataca
     2761 agatataaaa tatagagatt ttaataaaaa gcttatacct ggaacagaaa atataatagg
     2821 tattagagtt cctaatttaa gaaagttatc aaaagaaata agccaaggaa attggaagga
     2881 atttttagaa gtagcagagg atacatacta tgaagaagtt aggctacaag gtatggtaat
     2941 tggcaatata aatagtaatt ttgaagaaac tttatactat gtaaaaagat ttatacctaa
     3001 aatcgataat tggtcagttt gcgatggatt ttgttctgat ttaaagtcta tgaaaaaata
     3061 taaagaaaat atgtatgata tacttaaaaa atatgtttat tcaaagaatc catgggaaat
     3121 tagatttgca ttagttatgt ttttaatata ttatgttgat gataaacata ttaaagaaat
     3181 atttgaatac tgtaataata tacaaagtga agaatattat gttaaaatgg gtatggcatg
     3241 gttgctatca atttgtttta ttaaatgtga agaggaaact tttctctata taaaaaataa
     3301 taatttagat gatttcactt ataataaaat gctacaaaaa ataattgaat caaatagagt
     3361 agatttagaa aagaagaata taataagaag tatgaaaaga aagacaagaa gatgttgacg
     3421 gaggaatatt aatgaataac aatgaagaca gaattgacaa atttttagaa cttcaaagca
     3481 aaggatttaa atttgcagag atagctcaaa atctaaagat tagtcaaagt actttaagaa
     3541 cttttttaaa taaaaaagga tataagtcag aaaaaggtaa atacatatta aaagacgaat
     3601 taaattataa taaaagtttg gataatagaa acttggaaga agagaaagct aaggagtatg
     3661 aaaaaagtaa aaagaatact ataagtaata gaaaaaaagt aagcaccaaa aaaaagattt
     3721 ctaatgaaaa ggctttaagt aaaagtgttg aacaaattga gtttaatgat acaggtttaa
     3781 aaaatgcaaa gccaaaacaa aatgttaagc ctaagaaaga tagaaaaata aatataactc
     3841 aagaagattt agataagctt tgtgaagtat atgattggta tttagaagta aaagattaca
     3901 atataaagcc taaaaaagta tctaataaaa aagatgtaca tatagatgat gaacacatag
     3961 atgaattaaa aacaacaaga atacgtatag ataaagaaat atggaatgat tttgaaagat
     4021 tatgtagtaa ttcgaatttt gaaaaacatc aaatacttac acaggcattg aaagaattta
     4081 tgaaaaagta taaaaatcta ctataaaata ctactatatg aggtgcttaa tatgaataaa
     4141 atagtaaaaa aatcaatttc tctaatgatt atattaacaa tatttatttt tatgttgaca
     4201 gcatgtgaaa aagatgaaca gcctgattca gttgatattc aaacagaaga taaaaatgag
     4261 ataaaaatag atgagggaaa tgcaaaagta ttgaatatag gaaaatcaga aattataaat
     4321 atagatgaag gtgataaagt agatacatca acaaaggtag aaaataattc aacatttaat
     4381 atatccaatg ttgaattgat atataatgaa tatgatgaga ataaaaaaat tatatcttca
     4441 gatagtaagg ctttgttgga catgacttta atgcctggga aagttgctta catagaatgt
     4501 gggcataaaa cttttgtaaa gggtgtagaa gtttatgcat atgaatatga ggcagagggt
     4561 aagattgtgt atgttaattt aaaagaaaat actattaata taagaaataa taatattaag
     4621 ctagaaaata gtagtcagta tgaggtgcta tctacatcag aacttaaaaa agtaaatgaa
     4681 tcaaatgagg gaattacgta tcaagtaaaa gtaaaaaatt catcatcaaa agatttagga
     4741 aatattattc ttaagacagc agaagttaat gaaaatggag aatatttaac agtaaatcgt
     4801 gtgccatcat ataagatttt aaagccttca gaagagacag atatagatat attgtgttca
     4861 acaaaggcaa aaagtgttga aatagtggga tatacgtatg atgatataaa agaaaaagcc
     4921 aatgtggata ttgacttaaa atctcataaa gttaagatag acaaataaag gtaggggata
     4981 cttatgaagg ttttaatagt tgaagataat aagatacttt tagaaagtgt agtagaagaa
     5041 cttagcaagc attttgaaac tgaaaagtgt gaagatggag aagaagcttt atacttagta
     5101 aatcaaaata tatatgattt agtcatttta gacttaatgc ttcctaatat aaatggattt
     5161 gatatcctaa agaaaatgag agttaataat atagatacac ctgtacttat attgacagca
     5221 aaagaaacat tagatgacaa agtagaagca tttactatag gagctaatga ttatttgaca
     5281 aaaccttttt atatggaaga gttagtagct agggtatacg ctatattaag gactaatgga
     5341 aaaataaaag aaagaaacgg attagaattt aaatctttat atttagatac actagaaaaa
     5401 agagtgtata ttgaaaaaga agaaataaaa cttcaaaaca aacagtttaa tttgcttgag
     5461 tattttgtat taaataaagg ttcaatttta ttaaaagagc agatttatga tagaatatgg
     5521 ggaattgatt cagatgcgac tattgaaata gtggaagtat atgtaagtaa tctaagaaaa
     5581 aaactaagta aatatggata tgataaatat attaaaacta aacgtaaggt aggatatata
     5641 ttcgatgata aataagaatg tatttaccag tactaaaaat catttaataa aaatgtatat
     5701 tattgtagta ggtagttttt taataatctt ttctatattt atatactcat attttagggg
     5761 acttacatat agtggaattg atagtgagat aaatgatgaa cttgaatata tagtctctca
     5821 atttaaaaga acttcatttt tgaacccaat aagactgaaa gatcctaaag atatggtgta
     5881 tgtatatgaa gatggaagga taagttacta tactcaaaat gaatattttg atgagttact
     5941 tccagataga agactagata aaaaaaattc gttttttaaa tacacagaaa atggatatac
     6001 ttttagagaa ttaaatgtag atgtaggtag ataccaaata cagataatta gaaatataga
     6061 ttctgagatg aattctttaa gacagcttac atctgttctt ataattggaa tattaatttc
     6121 tgtaataatt acttactttg tggctgtata tctcactaga aaagctttaa ttccaataga
     6181 aacagcatgg aaaaatcaag ctaagtttat acaagatgct tctcatgaat taagaacacc
     6241 aataacaata gtttcatcaa aattagaaag tatgttaaaa tccccagaaa gtacagtaaa
     6301 tgatgaagtt gaaacaatag ctactgcaat gaaggaaact agaagactta aaaaaatgat
     6361 aactgattta ttgtctctta ctaaagagga ctctattgtt aaagttaatt tagaagaaat
     6421 agatttagaa aagttattgg aagaaatatc agaagactat attgacattg ctgagtttca
     6481 ggaaaaaaga ttcgtgttta attctaaact gaagaataaa gttataataa cagataaaaa
     6541 taaattaaga caacttatat taattttcat tgataatgcc tttaagtata caaaattggg
     6601 tgatgagata gcattagaat tgaaagaaga tatagaggat gaggtaactt tacttataag
     6661 tgatactggt ataggcataa aaaaagaaga gatacctctt atatttgata gattttttag
     6721 aagtgaaaat gtaagaaata aagatttaga aggttctgga ataggacttt caatagctcg
     6781 catgattagt ttaaacttat ctattgatat aaatgtaact tctgatgtag atattggaac
     6841 tacatttgaa ttgtcaatac caaaaaagtt aaaataataa aaatataaaa aattgttttt
     6901 agaaatgaag aatgggtata ctaattagta taaaatataa aaagacctta taaaagtaaa
     6961 tttatattta taaatttcat agtataatta tatagggata ataaagttta ataggaggat
     7021 ttaaaaatgg caaaagttat aaatacatct gaatttagaa gtagtgttga aggtagtaag
     7081 ggagtagttg tagttgattt ctttgcaact tggtgtggac cttgtaacat gttgggacct
     7141 gtatttgcag aattaggtga agagatgaag gataaagcta gatttgtaaa agttgatata
     7201 gatgagagct tagaaatagc tcaacaattc aatgtatcta cagtaccaac tatgattata
     7261 tttaaagatg gaaaaccagt agaaacttta attggattca tgccaaagaa caaaattgaa
     7321 atgcaagtaa aatcatattt ataaaaaatg ggtgagtatt atgagatatg atatagctat
     7381 aatcggcagt ggtccagctg gattgtctgc tgctataaat gcaaaaataa gaaataaaac
     7441 aattatcatg tttggtaatg ataatttaag taataagctt gttaaagctc cttcaataga
     7501 taattattta ggtttttatg atataagtgg tgatgaatta aaagacaagt ttaaatcaca
     7561 tatagatagt atggatattt caattgaaaa taaaagaata aataatattt atgctatggg
     7621 agaatacttt acaataatgt ctggaaatga tatgtatgaa gctactactg taatccttgc
     7681 aactggggta gagtatacta gaccaatcaa gggtgaagaa gaatttctag gaagaggtgt
     7741 gggctactgt gctacatgtg atgcaccact ttatagaaat aaaaaggtag cagttatagg
     7801 atataatgaa gaatctaaag aagaagcaaa ttttttaagt gaactcactt caaagactta
     7861 ttttatacct atgtacaaaa aagataatct tatgagaagt tcagataacc tagatgattc
     7921 tattgaagtt atacatgaca gaccagtaca gatagatgga gataaacttg taaacaaagt
     7981 atcttttaaa gaaaaccaca ttgaagttga tggagtattc gttataaaag atagtacagc
     8041 tccaagtgca ttggttcctg gaatagagat tgatggcatt catattaaag ttgacaataa
     8101 tatgaagact agtatagatg gatgctttgc tgctggagat tgtgttggaa aaccctattc
     8161 ttatataaaa gcagctggtc aagggcaaat agcagcttta aatgctgttt actatttaga
     8221 taaattaaag agggc
//
LOCUS       DAGSWI010000162         8171 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.165, whole genome shotgun sequence.
ACCESSION   DAGSWI010000162 DAGSWI010000000
VERSION     DAGSWI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.165"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            405..926
                     /locus_tag="MJF80_003409"
     CDS             405..926
                     /locus_tag="MJF80_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBZ0282037.1"
                     /translation="MDFKIRELINDITQDIIQTYKIQIPIVNINQVVDALGGKVIEDS
                     SLSGYSDGFIRKVDDSFEIVVSPYQPDTRKNFTIAHELGHLFLHMGYGIDDELWNSQD
                     GNQYFRSGNTNKEYQSNEFAAALLMPKHEYKRIMDENTVGNKVDTSKIAEYFNVSSYA
                     ASNRGKWLGYLQW"
     gene            902..2488
                     /locus_tag="MJF80_003410"
     CDS             902..2488
                     /locus_tag="MJF80_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282038.1"
                     /translation="MVRIFTMVDDKEYKTQNVNNVHNSAKNENAFNLKKYKEKLQENI
                     NTDIYEKEKEPNHPEVILFFSFDIANSSLYKNINYSGWAKVLSHIIRKLQYRVYENLK
                     AQLWRVLGDEVIFIIVLKNYDEIYKYIDIIFDILTSTAKDIKSGNIFSTLEGFSESEK
                     YLMKLQNIISLKGAAWIAIVSRNPNFNALENNEQYENISAMYDLSNNYKIFEFLGNDI
                     DAGFRISKQTCPERLVLSFELAYILSRKTDILSKLHIITYKKLKGIWKDKLYPIIWYH
                     NKGKNNDIEFDDSFSFDEIEENELVREYFFNKKGESKLLIDSFMFNSVDKALDKILID
                     RNLSDKIEKIGDVISKTNPSYDKNTIDKDYIKVDLMELHCVAVCYNKSTSKILIAKRS
                     DNRNNNASKWEFGCAKASLETSIINTIKDEYEKDFNINIEPITDCTRKDDCQPIPLAI
                     YQVKKSDGLHKGIITLAEIINDYDISKFEPTSKHNELAWIGEDELEDFNENTVPDFKE
                     TLKLAFKKLNENQLQESTNM"
     gene            complement(2552..3070)
                     /locus_tag="MJF80_003411"
     CDS             complement(2552..3070)
                     /locus_tag="MJF80_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282039.1"
                     /translation="MDKKIDIAKFIVNRIDYYIEKSDNKASFLLLLNSAIIGFLFSGK
                     EKIMNHLSISNIRCLEMLFYIVILCIFVISIYFSIMVLKPRNSKIENEYKSIFYYKEI
                     ASLNNEQYKEAFENAFKDEENLINDALVQIKELSLICDKKMYNVKKSVEAFILGGIIL
                     FIYILVVVFNAI"
     gene            complement(3236..3598)
                     /locus_tag="MJF80_003412"
     CDS             complement(3236..3598)
                     /locus_tag="MJF80_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282040.1"
                     /translation="MEDLCMDALVYERFVRAAFSIKLNNLINRSEDLGGLAEADIFRA
                     ANNLHELNEIKIGTGYAIAIFNNEILESCNVSDNDSNRMIELFDRSLIATSREEILDI
                     IREYETYRGRYLTFNWKR"
     gene            complement(3679..4491)
                     /locus_tag="MJF80_003413"
     CDS             complement(3679..4491)
                     /locus_tag="MJF80_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBZ0282041.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKNEEKSYKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSN
                     KGLEYATRICDKLGTVFKNRGAKLDKRLYILNSSKPTAVLIESFFCDNKEDYDKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVVGGGACEKISSITKEKFIMIKGNDRFDTLYKALDFINR"
     gene            complement(4491..4748)
                     /locus_tag="MJF80_003414"
     CDS             complement(4491..4748)
                     /locus_tag="MJF80_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBZ0282042.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLSPVAVLQGVICWGISIGINQTYKQLKEENK"
     gene            complement(4768..4998)
                     /locus_tag="MJF80_003415"
     CDS             complement(4768..4998)
                     /locus_tag="MJF80_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBZ0282043.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQRGIF"
     gene            complement(5030..5167)
                     /locus_tag="MJF80_003416"
     CDS             complement(5030..5167)
                     /locus_tag="MJF80_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBZ0282044.1"
                     /translation="MNWYKIITDFYNNGNWTKEQVKTAVEKNKITASEYKEIVGEDYI
                     A"
     gene            complement(5168..5467)
                     /locus_tag="MJF80_003417"
     CDS             complement(5168..5467)
                     /locus_tag="MJF80_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282045.1"
                     /translation="MIYLGNLMDTEEQNIKYVGMIHYEPNLLSEENLKQGILIEELPT
                     PKYTENKETKLFINIDTKEVFYRYTDIKSSIEDKVNSTEQTIADLTFQLMSNGVI"
     gene            complement(5464..6696)
                     /locus_tag="MJF80_003418"
     CDS             complement(5464..6696)
                     /locus_tag="MJF80_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282046.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENNFTSDNVEGALKEVFQS
                     GVNAKNNVVTALNSKGAEVTTSDTWEEIKNKIDIKEGRLDLRETTLSNNYPYLVTNGA
                     IKYIERCSGNFKTFEYEEPYFYVIKETHLIKINAIDETVVFDITLANANFSCICVTQE
                     YLFISDNTKLYKINKTTGIEVQSIEGAYYKLCTYGEFIYGVYGDETSSTLHKIRISDM
                     YIMLTKNLVSNGIYNFKGGKFVCNKNAIYATTEHSNSSSITTCHLSKINFDFAIAKDF
                     RIGGYLHMKNIKFLNDFVIASDAERGIEIDSNKKSALVKYDANLNLIVYSDDSKYDNF
                     DIYNGYIYAIYSLSSSPFVKISLNTLKRIDSYRKLTETYPSAGMFVINDIVFFISSGI
                     FRNILSKKVYSDEKGESL"
     gene            complement(6731..6913)
                     /locus_tag="MJF80_003419"
     CDS             complement(6731..6913)
                     /locus_tag="MJF80_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282047.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGIEPTA"
     gene            complement(6913..7209)
                     /locus_tag="MJF80_003420"
     CDS             complement(6913..7209)
                     /locus_tag="MJF80_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282048.1"
                     /translation="MNVPNRVIYDQTGKIIFETGESCGDVLPHNKITELHYIDIPYGS
                     IDYIKNRIVGINIETKQPILEEIPVYVSEAEKEKQELENQLLLLTNQEIGGGIL"
     gene            complement(7224..>8171)
                     /locus_tag="MJF80_003421"
     CDS             complement(7224..>8171)
                     /locus_tag="MJF80_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282049.1"
                     /translation="LITLLQALPFLTVSSYNSTQATFKADHTDPTLLTKIEVFIDDVL
                     KETITTDLTTEKTINYTLEDNALHTLKIVVTDSNNATAEKVLSISKNIMPLPENVNLN
                     DISTKLVEVNAGFKVGKTSIINTLALKNIEASLNNTLVELSEKIKTSFDSSDASVQDL
                     MNQLTQANNTITQLNTKYKVASGTVTPFKGDDTKIAYPYLTDRVFKPSAWVKISNLDF
                     KPNIFFADFDYYDTEYKNNYKLFLFACCGVATQRGVDFSSVTSFVRKSSDEYFHANGW
                     LYNNSEGDVYFNNTGVQIPAYSFDSTQRHIYKWYAIKFI"
BASE COUNT         2853 a         1268 c          921 g         3129 t
ORIGIN      
        1 caacttctac tatagttttc atactttaac atcaactaaa tgaatttaat taagattact
       61 agttaatcgt tttttggttc tttcatattc tgaaatctaa aattttatta tttgtttatt
      121 gttattattt aacacatgtt ggtatttcaa cgatttatct tattgttaat attcttattg
      181 cttccaaagt atatctaatt atttttaaga taatttacta atttttattt tttcaaacat
      241 acattcgaca aaaaacagtt tttatatggt ataattatat tgtataatac aaaaggttaa
      301 tgaaaataat taatattaaa tgtaccaaaa aaaatagttt ttgatataat gaatattata
      361 ataaataatt atttaaataa aatattatat aaggatgtga ttttatggat ttcaaaatca
      421 gagagctaat taatgatata acccaagata ttatccaaac atacaaaatc caaattccaa
      481 tagtaaatat aaatcaagtt gttgatgctt taggaggcaa ggtaatagaa gatagttctt
      541 taagtggata ctctgatgga tttattagaa aagttgatga ttcatttgaa atagtggtat
      601 ctccttatca accagatacc agaaagaatt ttaccattgc tcatgaactt ggacatttat
      661 ttttacacat gggttatggc attgatgatg aactatggaa tagtcaagat ggaaatcagt
      721 attttagaag tggcaatacc aataaggagt atcaatccaa tgaatttgca gcagccttgt
      781 tgatgcctaa acatgaatat aaaagaatta tggatgaaaa cacagtaggt aataaggtcg
      841 acacttcaaa aattgcagaa tactttaatg tttcctctta tgcagcatct aatagaggga
      901 aatggttagg atatttacaa tggtagatga taaagaatat aaaactcaaa atgtaaataa
      961 tgttcataat tcagctaaaa acgaaaacgc ttttaatctt aaaaagtata aggaaaaact
     1021 tcaagaaaat attaatactg atatttatga aaaagaaaaa gaacctaatc atccagaagt
     1081 gattttgttc ttttcttttg atatagctaa ttcatcatta tataaaaata taaattatag
     1141 cggatgggct aaggtattat cacatataat tcgtaagtta caatatagag tttatgaaaa
     1201 ccttaaagca caactttgga gagttcttgg tgatgaggta attttcatta tcgtactaaa
     1261 aaattatgat gaaatttata aatacataga tataatcttt gatattttaa caagcactgc
     1321 taaagatata aaaagtggta atatattctc tacactagaa ggattttctg aatctgaaaa
     1381 atacttaatg aaacttcaaa atattatttc attaaaagga gcggcctgga ttgctatagt
     1441 atcaagaaat cctaatttta atgctttaga aaataatgaa caatatgaaa atatttctgc
     1501 tatgtacgat ttatccaata attataaaat atttgagttc ttaggaaatg atattgatgc
     1561 tggtttcaga atatcaaagc aaacatgccc agaaagactt gttcttagtt ttgaactagc
     1621 ttatatatta tcaagaaaaa ctgatatttt atctaaatta catataatta catataaaaa
     1681 attaaagggt atctggaaag ataaactata ccctatcatt tggtatcata ataaaggaaa
     1741 aaataatgat atagaatttg atgatagttt ttcttttgat gaaatagaag aaaatgaatt
     1801 ggttcgagaa tattttttta ataaaaaagg agaaagtaaa ttactaattg attcttttat
     1861 gtttaattct gtagacaagg ctttggataa aatacttata gacagaaacc ttagtgataa
     1921 gattgaaaaa ataggtgacg taatttctaa aacaaacccc agttatgata aaaatacaat
     1981 agataaagac tatataaaag tagatttaat ggaattacac tgtgttgcgg tttgttataa
     2041 taaatcaact tcaaaaatat taattgcgaa aagaagtgat aatagaaata ataatgcgag
     2101 taaatgggaa tttggttgtg ctaaggcaag tctagaaact tcaattataa atactattaa
     2161 agatgaatat gaaaaagatt ttaatataaa cattgaacct attactgatt gcacaagaaa
     2221 agatgattgc caacctatac ctctagcaat ttatcaagtt aaaaaaagtg atggtttaca
     2281 caaaggcatt ataactctcg cagaaataat aaatgattat gatatttcta aatttgaacc
     2341 cacatcaaaa cataatgaac ttgcatggat aggagaggat gaacttgaag attttaatga
     2401 aaacacagta cctgatttta aagaaacttt aaaactagca tttaaaaaat taaatgaaaa
     2461 tcaattacaa gaatctacaa atatgtaaat agtattcttc tatgatttga ttctatcttc
     2521 atagaggaat actaaccaaa taaaacattt tctaaatagc attaaaaact actacaagta
     2581 tatatataaa caatataata ccacctaaaa taaaagcttc tactgacttt tttacattat
     2641 acattttctt atcacagatt aaagataatt cttttatttg aacaagtgca tcattaatca
     2701 agttttcttc gtctttaaat gcattttcaa atgcttcttt gtactgttca ttatttaaag
     2761 atgctatctc tttataataa aaaatagatt tatattcatt ttctatttta gaattccttg
     2821 gctttaaaac cataatcgaa aaataaattg aaataacaaa tatacataat ataacaatgt
     2881 aaaatagcat ctctaaacat ctgatattac ttatacttaa gtgattcatt attttttcct
     2941 tccctgaaaa taaaaaacct ataattgcac tatttaataa tagtaaaaaa ctagctttat
     3001 tatctgattt ttcaatatag taatcaattc tatttactat aaacttcgct atgtctattt
     3061 ttttatccat gattttacta cctcatttca ttaaatttta tattgattca aaattttatt
     3121 ttctacttat tatcattgaa taaattatta tttggaaata atcttattga aataataaaa
     3181 atatgtgaaa gaaagtaata actaactata attactactt cctaacttaa acattctatc
     3241 tcttccaatt aaaagtaaga tatcgtcctc tataagtttc atattctctt ataatatcca
     3301 atatttcctc cctagaagtt gcaattaaac ttctatcaaa tagttcaatc attctattgc
     3361 tatcattatc agaaacatta caagactcta atatctcatt attaaatatt gcaatagcat
     3421 acccagtacc tatttttatc tcatttaatt catgtaaatt atttgctgct ctaaagatat
     3481 cagcttcagc caatccacct aaatcttcgc ttctattaat caagttatta agtttaatac
     3541 taaaagctgc tcttacaaat ctctcataaa ccaatgcatc catacataaa tcctccatta
     3601 tcacaataat atatttacat tatattacaa taatagagtt atcacaacaa acaaccactt
     3661 ctagttgata acttttatct atctattaat aaaatccaat gctttataaa gtgtatcaaa
     3721 tctatcatta ccttttatca taataaattt ttctttagta atagaactta tcttttcaca
     3781 tgcgccacct cctacaacat aaagattttg cgtctgacct ggcacgtaat cttttatatc
     3841 acatatcagt attttcccat cattataacc ccaaccaact acagttgcag agattttgtc
     3901 aacttctcca tcataaacaa ttgtatgttt gtacatctgt ttaactccct cattatttat
     3961 atttttattt aatacacctt ctacaattaa cttagcaata ccttcatgac ctagtttctt
     4021 agctttatca taatcttctt tattatcaca gaagaaactt tcaattaata ctgctgtagg
     4081 ctttgaacta tttaagatat ataatctttt atctaattta gcacctctat ttttaaatac
     4141 tgtacctagt ttatcacata ttctagttgc atactctaag cctttattac tataatatag
     4201 gacttctgaa cctttacctt gaccgttact tgcatttaaa tgtaactcta taagtaaatc
     4261 atatcctcca ctattaactc taggtatttt ataagacttt tcttcattct tagttttaaa
     4321 ctgcttttct gggcatatta ttacatctac cttatgccct tcttttctaa atgtatctgc
     4381 taatactggt gcaagagatt tgttgtattg atactcgtta actactccat cagcagaagt
     4441 acatgctcca ctttttaaaa tactgtgtcc tactgttata catattttca ttatttattt
     4501 tcctccttca actgtttgta agtttggttt atacctattg atatacccca acaaatcacg
     4561 ccttgtaaga ctgcaacagg acttagtcct aacatccaaa tagaaaatcc tataccaagt
     4621 attaataaca ctactggaat atatttgtta tctagttgct tatacttctt acagccctta
     4681 cctataatag agagagcagc tactaaaatt agcaactgct ctggtataaa acttattaaa
     4741 ttatccatct cttatcctcc taattaatta aaatattcct ctttgaactg caaatataaa
     4801 gaaccctaca agtgttgtaa tcattgtacc aattagccat ttgagcatac ttgtaagtga
     4861 gtttagattc tcacacaatg cttttaactc tgctttagac tctatatttg ctatttttaa
     4921 ttcgtctatt tcttctccat gtttatttat tcttgtttca tgtcttttta aatctgcttc
     4981 gaaaagttct tcattcatga aaacctccta atttttgaat taaaaaagac tatgctatat
     5041 agtcctctcc tacaatttct ttatattcac ttgctgttat cttattcttt tctactgccg
     5101 ttttaacttg ctctttagtc caattaccat tattatagaa atctgttatt atcttgtacc
     5161 aattcattta tatcacccca tttgacatta attgaaatgt taaatctgct attgtttgtt
     5221 ctgtagaatt tactttgtct tctatgctac ttttaatatc tgtatatcta tagaaaacct
     5281 ctttagtatc tatatttata aataactttg tttctttatt ttctgtgtat tttggtgttg
     5341 gtaattcctc tatcaaaata ccttgtttta agttttcctc cgataacaaa tttggttcat
     5401 agtgtatcat accaacatat tttatatttt gttcttctgt atccattaaa tttcctaaat
     5461 aaatcataat gattctcctt tctcatccga ataaaccttt tttgacaata tatttctaaa
     5521 aattccacta ctaataaaga aaactatatc atttattaca aacataccag cacttgggta
     5581 tgtttcagta agttttcggt agctgtcgat acgtttaaga gtatttaaac ttatttttac
     5641 aaaaggacta ctagaaagcg aatatatagc atatatatat ccattatata tatcaaaatt
     5701 gtcatatttg ctatcgtctg aatacacaat taaatttaaa tttgcgtcat attttactaa
     5761 agcacttttt ttgttgctat caatttctat acctctttct gcatcagatg caataacaaa
     5821 atcatttaaa aacttaatgt ttttcatatg caaatatcct ccaattctaa aatctttagc
     5881 aatggcaaaa tcaaaattta ttttagataa atgacatgtt gtaatactac ttgaatttga
     5941 gtgttctgtt gtagcataaa tagcattctt attgcaaaca aacttacctc ctttaaaatt
     6001 gtaaatacca ttagaaacta aatttttagt taacattata tacatatcag atattctaat
     6061 tttatgaagt gtagaagaag tctcatctcc atatactcca taaataaatt ccccataagt
     6121 acataactta taataagcac cttctattga ctgcacttca attcctgttg tcttatttat
     6181 tttatataat ttagtattgt cagatataaa taaatactct tgtgtaacac agatacatga
     6241 gaagttagca ttagctaaag taatgtcaaa aactactgtt tcatcaatag cattaatttt
     6301 aattagatga gtttctttaa ttacataaaa atatggttct tcatattcaa aagttttgaa
     6361 gttcccacta catctttcaa tatattttat agctccattt gtaactaaat acggatagtt
     6421 atttgaaagt gttgtttctc ttaaatctaa tcgcccctct tttatatcaa ttttattctt
     6481 tatttcttcc catgtatcgc ttgtagtaac ctctgcacct ttggagttta atgctgttac
     6541 tacattattt ttagcattaa ctccactttg aaaaacctct tttaatgctc cttctacatt
     6601 atcacttgta aaattattct ctgtatcttc tatagttaca ttctttgctt ctaatacaag
     6661 atttctaact ttattaacta actctttaaa agtcatttag tcaccttctt tcaataaaaa
     6721 aaagaaccta ctacgctgtt ggttctattc cttctactac tccactattt tttataatat
     6781 aatcctctac tgcttttctg tattctgtgt tagttacatc atcaagttga aactctctat
     6841 ttttcaaagg gtttaaccct ccatttaata ttctttctgc taatattctt accacaacat
     6901 tatttatatt cattataaaa ttcctcctcc gatttcttga tttgtcaata aaagtaattg
     6961 attttctaac tcttgttttt ctttttctgc ttcacttaca tatactggta tttcttccaa
     7021 aattggctgt tttgtttcta tatttatacc tacaattcta tttttaatat aatctatact
     7081 tccatatgga atatcaatat aatgtaattc agttatttta ttgtgtggta acacatcccc
     7141 acaggactca cctgtttcaa aaattatttt acctgtttgg tcatatatta ctctatttgg
     7201 aacattcatg tttcttctcc ttcttaaata aatttaatag cataccattt ataaatatgt
     7261 ctttgagttg agtcaaagct ataagctgga atttgaacac cagtattatt aaaataaaca
     7321 tctccttcgg aattattata gagccagcca ttagcatgaa aatactcatc actacttttt
     7381 ctaacaaaag atgtaacact tgaaaaatca acacctcttt gtgtagcaac accacaacaa
     7441 gcgaaaagga aaagtttata attattttta tattcagtat catagtaatc aaaatcagca
     7501 aagaaaatat taggcttaaa atctaagtta ctaattttaa cccaagcgct aggtttaaaa
     7561 actctatcag ttagatatgg ataagcaatt ttagtgtcat cacccttaaa aggagtaact
     7621 gtgccacttg cgactttata ttttgtattt aactgtgtta tggtattatt agcttgtgtt
     7681 aattgattca tcaaatcctg cacactagcg tcagaactat caaaacttgt ttttattttc
     7741 tctgataact cgactaatgt attatttaaa cttgcctcta tattctttaa tgctaaagta
     7801 tttataatgc ttgttttccc aactttaaat cctgcattaa cctcaactaa ttttgttgat
     7861 atatcattta aatttacatt ttcgggcagt ggcattatat tcttacttat acttaaaact
     7921 ttttcagcag ttgcattatt gctgtctgta acaactattt taagcgtgtg cagtgcatta
     7981 tcttctaatg tatagttaat tgttttctct gttgttaaat ctgttgttat agtttctttt
     8041 agtacatcat ctataaatac ttctatctta gttaatagtg tagggtctgt gtggtcagct
     8101 ttaaatgttg cttgtgtgga gttataagag gatacggtta aaaaaggtaa tgcttgtagt
     8161 aatgttatta g
//
LOCUS       DAGSWI010000163         8112 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.166, whole genome shotgun sequence.
ACCESSION   DAGSWI010000163 DAGSWI010000000
VERSION     DAGSWI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.166"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..1181
                     /locus_tag="MJF80_003422"
     CDS             <1..1181
                     /locus_tag="MJF80_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBZ0282050.1"
                     /translation="DVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDND
                     HFILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDL
                     LIDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKY
                     SLDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKW
                     INKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNIL
                     IDIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIIS
                     KIVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD
                     "
     gene            1328..2074
                     /locus_tag="MJF80_003423"
     CDS             1328..2074
                     /locus_tag="MJF80_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282051.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            2074..3954
                     /locus_tag="MJF80_003424"
     CDS             2074..3954
                     /locus_tag="MJF80_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282052.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            4073..5464
                     /locus_tag="MJF80_003425"
     CDS             4073..5464
                     /locus_tag="MJF80_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBZ0282053.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            5571..6611
                     /locus_tag="MJF80_003426"
     CDS             5571..6611
                     /locus_tag="MJF80_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBZ0282054.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            7100..7753
                     /locus_tag="MJF80_003427"
     CDS             7100..7753
                     /locus_tag="MJF80_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBZ0282055.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            7829..8038
                     /locus_tag="MJF80_003428"
     CDS             7829..8038
                     /locus_tag="MJF80_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282056.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
BASE COUNT         3252 a          765 c         1372 g         2723 t
ORIGIN      
        1 tggatgtcaa taaatttaaa tatataaatg agatgtttgg atatgctgaa gggaataaga
       61 tattaattca tatatcacag gttttaaata ataatacaaa taaatatgaa atttgtgcta
      121 ggatggataa tgaccatttt atattgttga ttgcttgtaa cactgaagat gaacttttaa
      181 atagattatc taaaataaat aaagaaattt gtaacttaaa tactactaac tcatctaagt
      241 ataaaattgt tatgtcaagt ggaatatata agattactaa aaaggatgat attaaaaaga
      301 ttgacttgtt aattgataga gcaaatattg cagcaaaaag taaaaaggaa aaatatgaac
      361 attcatattc attttttaat gaagatacaa gaaatcgatt gtacaaggaa aaacgattgg
      421 aagataatat gaataaagct ttagaaaaag gtgaatttat agtatattat caacctaagt
      481 atagtttaga tgatgtaaat gaaattgaag gagcagaagc cttgataaga tggaatagtc
      541 cagaatttgg gtttatttca cctatagatt ttgtaccttt atttgaaaag aatggtttta
      601 ttgtaaatat tgatatgttc gtgtttgaag aagtatgcaa aacattaaac aagtggataa
      661 ataaagggta tactcctgta ccaatctctg taaatatgtc tagagttcat ttatataggg
      721 ataactttat agaaaatata actgatttga taagtaaata caatatatct cctgagttta
      781 ttgaacttga actaacagaa agtgttgtgt ttgataattt aaatatattg attgatataa
      841 tgaaaaagat taaagagata ggattcctta tatcaatgga tgattttggt tcgggatatt
      901 catctttaaa tctgttaaaa gacctttctt ttgatatttt aaaattagat agaggatttt
      961 taatcgaaac aacagataca aaacgtggaa aaattattat ttctaagatt gtggaaatgg
     1021 caaaagcgat tgatattaaa gtaatctgtg agggcgtaga aacttatgaa caagtagagt
     1081 tcttgaaaga gataggttgt gataaggttc aaggatactt atttgctaaa cctatggtat
     1141 tagatgaatt tgaaaaacac ttaaacttta aatttgatta atcattaata attattagac
     1201 tataagatac cctaacaact tacttacaaa tatgtaagtt aattgttagg gtatttttat
     1261 ggttaaagta gcatattcac gatattatgg tattgtagta aaaataaaaa attatggaga
     1321 atataaaatg aacatattga gtattagaaa tatatcaaaa acatattgtg gaaatattcc
     1381 atttaaagca ttagataagg ttagtctaaa tatagaaaag ggagaatttg tgtctgttat
     1441 gggaccatct ggaagtggta aatctactct tttaaatata atatctacag ttgatagaca
     1501 gagtgaaggt gaggttgtat tagatggata tgatgtaagt aaattgaaag gagaaaaact
     1561 tgctgaattt agaaggaaac aacttggttt tgtatttcag gattttaatt tgattgacac
     1621 tttaactgtt ggcgaaaata ttatgttgcc acttacacta gagggggcaa gtatcaaaga
     1681 tatgaatata caaactaaaa gtatatctaa atttttagga atagataaaa ttttagatag
     1741 aaaaacctat gaaatttcag gaggtcaagc tcaaaggtgt gctattgcta gagctattat
     1801 aaataaacct gccatacttc ttgcagatga gccaactgga aatcttgatt caaaatctac
     1861 tgatgatgtt ttgaaattat ttactaggat aaacaaagaa caaaaggtaa caacacttat
     1921 ggtaactcat gaagcatata gtgcaagtta ttcagataga gtaattttta taaaagatgg
     1981 ttgtatatat acagaaataa aaaaatctga aagcagtaat tctttctatt ctgatatact
     2041 agctgttttg tctcaaatag gaggtgtaag atagtggatt ttagaaagtt tgcttataat
     2101 aatatagtta gaaatttcag ggcatattta gcatattttt taagttctgt tttttctata
     2161 atgatatttt ttgtttttgc agtgtctatg ttccatccaa ttataacaaa atctggaatg
     2221 cagagtggtt ctactgcatt tatggctctt atgacttcag aattaataat attcggtttt
     2281 agtctacttt ttatattgta ctctttagga aatttcttaa aatatagatt gaaagatttt
     2341 ggagtattga tgataattgg aatgagtaac agacaactga aaaaattgat attaattgaa
     2401 aatttgataa taggattttt agcagtgatg ttaggaatat tacttggttt atcaatttca
     2461 aaattgtttt tattgtattt aagcaaactg ttttatatga atttaactga attttatttt
     2521 ccagtaaaag caatgctatt gactattgta tcatttatga ttttattttt gataacagga
     2581 ccaatgagct taaaattatt gagtaaaaag agcattttag agcttttagt aggtacaaaa
     2641 aagcctaaaa aagagataaa atcttcaaag ctatttggaa taattggtgt aatatgtctt
     2701 atattaggat atgctatgat actatttggc aataagttaa aaataaatgg aggtatgtat
     2761 acaacagcac tattaattac tttggggata tttttatttt tttctcaatt tactattttg
     2821 atattaaagt atttaaaaaa taaagaaaaa ttttataaga acaaagtaaa tatgttactt
     2881 ataagtaatt tgatatataa attaaaggat aatacacgat tgttattttt aataacaata
     2941 cttttatcgg ctactttagt agcatttaca acaacttcaa cacttgtaag ttctcaagga
     3001 gaagcttcta aaaataattt cccaatggtt tactcatatt tttcagagca taataatgaa
     3061 aaagaatatg aagaactaca acaaattaga gaagcaatca aaggatatga ttataaggag
     3121 ttaagttttc ctgtacttaa atataatagg gaagtcttac taagtgtaaa gaattataac
     3181 aaattatcaa aggaattggg attagataaa attagttcaa agttagaaaa atatgaaggg
     3241 attataattc ctagtagcaa ttcacaagaa catataaatt ctttaaaaca attaaaaaat
     3301 tatagtataa aaaatttaaa tatagaagta aaaaaatcaa aaaataaaat aatttttcct
     3361 acaggactgt ttagtaaagt tgtagttata agtgatgact tatacaatga agtaaaaggt
     3421 gaattttcaa aaattaattt tcatggattt gattataaaa actggcaaag cagtgcaaag
     3481 ataactgaaa atttaaaaga aaaacatttt aatttggata gagaatataa caggtcatat
     3541 tttttaacat taccagatat gtatttagta gagttacaac aaagtaaaat tcttcaattt
     3601 atgggtgttt ttataggagt gatattattt atagcagttt ttagtttttt atacttcaga
     3661 ttatatacag acgagataga agataatgta aagtacaaaa aactatcaaa aataggtcta
     3721 tcttttaaaa atatgaataa gattgttagt atagagatag gtacattgtt ttttataccg
     3781 tattttatag caattataaa tagcataatt tctttactgt ttttaaatca agtattaaat
     3841 aatagtttta agttagaaaa tttgggtata ttactactga tatcatctat atattcactt
     3901 tatttttact tattaaaaaa aatttatact agaaaagttt ggttttttga ttaaagtggt
     3961 atataataga tatataaatt aatatttaaa aagtagttaa tattagggga gggcatttgc
     4021 acctcccttg taatatgtgg aaaattgaat attagaagaa ggaggagact tgatggaaaa
     4081 tcaacaatta ttaggaacag aacgaattag taaattacta ctaaaatatt ctataccagc
     4141 tataataggt atgttggtaa atagtttata taatgtagta gatagaattt ttattggaaa
     4201 tataccaggg gttggaccac ttgcaataac aggtcttggt gtaacaatgc caataatgac
     4261 aataatactt gcatttggga tgttgatagg tataggaact actactacca tatcaataaa
     4321 acttgggcaa ggtaaagtag aagaggcaag aaaacttata ggaaatgcaa tgacgttatc
     4381 tgtaataaca ggaattatca taatgatact tggaatatta tttgcaaata aaatacttac
     4441 attgtttgga gcaagtgaaa atactttaat atatgcaaaa tcatatatca atattatatt
     4501 attaggtaca gtagtaaatc ttctttcctt ttctttaaac cactcaataa gagctgatgg
     4561 tagtcctaaa atttcagcag gaataatgat agtaggatgt ttaactaata tagttttaga
     4621 ctggatatta atatttggat tcaatttggg aatacaagga gctgcaattg ctacagtaac
     4681 ttctcaggct cttacagcta ttttaactat agggtattat ataagtggaa aatcaaactt
     4741 acgattcagt aaatctaatc taaaattaga taaaaagcta ataaaagcag tgtttgcaat
     4801 aggtatgtcg ccatttgcta tgcaacttgc agcaagttta gttcaagtaa tatcaaatat
     4861 tgcacttaaa actcatggtg gagatttagc tataggggct atggctacta tttcttctat
     4921 tgcaatggta tttttgatgc ctatctttgg aataaatcaa ggagcgcaac ctattatagg
     4981 tttcaattat ggagctgaaa aatatgatag ggttaaaaaa gcatatttag gttcattagt
     5041 agtagctact ataattttat gtatgggtat ggtagtagtt atgcttttcc cagaagctat
     5101 tataggtata tttaataaag accctgaact tatgaatata tctgttaatg gattaagaat
     5161 atacttactt atgttgccaa ttgtaggctt atcagttaca ggaacaaact ttatacaatc
     5221 tataggaaaa gcaaaaatgg ctatgttatt gagcctttta agacaagtaa ttttattgat
     5281 accagcagta ttaattcttc ctacattctt aggtttacag ggtgtatgga cagctcaacc
     5341 agtgtctgat tttatagcta cagtaattac aggtatagtt gtatttagag aattaaaaag
     5401 atatactcct aaaactgaaa aattaaatga aaatgaaaga ctaaacgaga taactactga
     5461 gtaaaaatat aaattcagat aattgagcaa gaaattgcaa aaaatacgat tatatgataa
     5521 aataaaaccg ctgtcaagga taagagaaat tctaattatg gagtgatttt atggaattta
     5581 caaagaaaag caaattaaaa aatatgtatg taaacatgaa tgtagctaga atgttgtcta
     5641 agataaatga gtataagggt agacaactgc tttacaaaaa acaaccaaaa gaaattttag
     5701 aaaacttaga aaaaaaatct ttagtagatt gttctgaatc tacatacatt ggaaatcaaa
     5761 gagatagtag taattttaat ctagaaaagt tgataagtaa tgaagttaca cctagaagta
     5821 gagaggaatt gagtattgtt gaatacagag atgtggtaaa aactattaac agtgcttatg
     5881 agagtattcc catcagttca caaacgattc ttgagcttca tggatactta tataaatttt
     5941 catctactag aggaggaagt tataagtcag ataatgattt tattgaacac aatttaaaac
     6001 ctagcgaatt tataagtact gattcaagtg tcaataaagt tgaaaaagct gtagaagaaa
     6061 tatgtgaagc ttataataca ttgatagagg aagatgaaat tgatatatta atattaattt
     6121 ctgcatttgt gttagatttc atattgatac atccattcaa agaaggtaat ataaaaatgg
     6181 cgagagtact tatcctattg ttactaaata aaaatgggta tgaagttgga agatatataa
     6241 gtttaggaaa gatatttgat gatagttcat atgaatatta tagtaattta aattatttga
     6301 aagcctcaat aggtagtgaa aaagcagata tgaatgcatg gattgagtat tttttagaga
     6361 cgatattaac tgcatatgag aagctggatg atagtttaaa tatatctgat aagaaaagac
     6421 aaacaaaaac tagtagaata gagaaaataa taaattctac ccttggatat tttacaaaag
     6481 aagatataag agatttatgt cctgacattc cagaacctac tataaataga gtttttaata
     6541 atctaagaaa acaagataag atagaagttg ttgcaagagg aagaagtgca aaatggaaga
     6601 aaaaatatta aaaaatgttt agataactaa aatggtatag tgttttacac tataccattt
     6661 ttaatgtcat tagcatgagc aataatttaa caacttaagc aatatcaatt ttatttattg
     6721 attatttttg aactgaaaaa tggcgttata tggtatacct cagtggtaat taattttgga
     6781 tgagaagata taatattaca gaatatatta ggagtatatg tagaaaaaat ataataaaag
     6841 tgtgaggctt agtaattaac aaattatgtg gcttcacact tttttataaa ataaagatac
     6901 ttagcagaat aatattttag ttgatatgtt tataagatgg tacttgtttg ggtaaaagta
     6961 taaatagtaa aaaagtagaa gtaatgtgta taaaaacata agttacattc tgtaataata
     7021 gttattttat aattcaacaa aagaatagaa tgggtatatt tgtgaaataa taatataaaa
     7081 taatcttagg agtgagctta tgaagaaaaa attattatat ataaatgtaa attcaaaacc
     7141 tgaagactta tcttcaagta aaactgtagc aagaaaattt ataaataaat ttatggaaaa
     7201 aaacaaagat tttgaggttg aagagataga cctttataaa gaacatatac caagacttga
     7261 gtatcagtac tttgaaaaaa gaaatagcat agttagtgaa gaaaatgcta aaaatttaga
     7321 cgataaggat agaaaagagc ttacaaagat tagaaatctt tgtgaccaat ttgtgagtgc
     7381 aagtgtatat gtaatagcag cacctatgtg gagtttatct tttccagcac cacttaaaga
     7441 gtatatagat tgtataattc aagatggaaa aactatatct tttgaaggca atgataaacc
     7501 acaaggaatt ttaaatgata ttgatagaag tatggtatat atacaatctt ctggtggaca
     7561 cataccatgg gtgttgaagc cagttatgaa taaagggtta aattatgtgg aaagtattat
     7621 gaaatttata ggaattaaaa aatttgatga attgttagta gatggaactg gaactagtga
     7681 agaagaaaga caagctgcaa tagaaaaagc atctgaaaaa atagatggta taatagatgg
     7741 gatgaaattt taattataat attttattaa aaatataaag gaaaaacctc ttttaatgta
     7801 gaaacttata ttgaaggagg cgagaatcat gcgagaagaa aaaagtaatg aaaagtatga
     7861 ttgttattgg tgtaatcaag agaataattt ctgtgtagaa ataaaagata atatagtcat
     7921 gatagatgat ggcactggta cgttaaaaca agccgttttc atagggtata aacaaatcca
     7981 aattaattta aattgttcac attgtcaaaa cttaaataga ataaaattaa atttgtagaa
     8041 ttttaaaatg gaaattattg aaggaattaa aaatagaaag tgttaatatt tagactgaaa
     8101 attatatttt at
//
LOCUS       DAGSWI010000164         8090 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.167, whole genome shotgun sequence.
ACCESSION   DAGSWI010000164 DAGSWI010000000
VERSION     DAGSWI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.167"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            27..101
                     /locus_tag="MJF80_003429"
     tRNA            27..101
                     /locus_tag="MJF80_003429"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="MJF80_003430"
     tRNA            116..200
                     /locus_tag="MJF80_003430"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="MJF80_003431"
     tRNA            210..293
                     /locus_tag="MJF80_003431"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="MJF80_003432"
     tRNA            317..391
                     /locus_tag="MJF80_003432"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="MJF80_003433"
     tRNA            416..492
                     /locus_tag="MJF80_003433"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            500..575
                     /locus_tag="MJF80_003434"
     tRNA            500..575
                     /locus_tag="MJF80_003434"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:533..535,aa:Gln,seq:ttg)
     gene            584..659
                     /locus_tag="MJF80_003435"
     tRNA            584..659
                     /locus_tag="MJF80_003435"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:617..619,aa:Lys,seq:ttt)
     gene            662..750
                     /locus_tag="MJF80_003436"
     tRNA            662..750
                     /locus_tag="MJF80_003436"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Ser,seq:tga)
     gene            754..829
                     /locus_tag="MJF80_003437"
     tRNA            754..829
                     /locus_tag="MJF80_003437"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:787..789,aa:Phe,seq:gaa)
     gene            836..909
                     /locus_tag="MJF80_003438"
     tRNA            836..909
                     /locus_tag="MJF80_003438"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Met,seq:cat)
     gene            924..1000
                     /locus_tag="MJF80_003439"
     tRNA            924..1000
                     /locus_tag="MJF80_003439"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:958..960,aa:Ile,seq:cat)
     gene            1018..1094
                     /locus_tag="MJF80_003440"
     tRNA            1018..1094
                     /locus_tag="MJF80_003440"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1052..1054,aa:His,seq:gtg)
     gene            1103..1178
                     /locus_tag="MJF80_003441"
     tRNA            1103..1178
                     /locus_tag="MJF80_003441"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1136..1138,aa:Lys,seq:ttt)
     gene            1186..1259
                     /locus_tag="MJF80_003442"
     tRNA            1186..1259
                     /locus_tag="MJF80_003442"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1218..1220,aa:Cys,seq:gca)
     gene            1267..1343
                     /locus_tag="MJF80_003443"
     tRNA            1267..1343
                     /locus_tag="MJF80_003443"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Arg,seq:acg)
     gene            1356..1431
                     /locus_tag="MJF80_003444"
     tRNA            1356..1431
                     /locus_tag="MJF80_003444"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1389..1391,aa:Val,seq:tac)
     gene            1507..1920
                     /locus_tag="MJF80_003445"
     CDS             1507..1920
                     /locus_tag="MJF80_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282057.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVYGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2229..5660
                     /locus_tag="MJF80_003446"
     CDS             2229..5660
                     /locus_tag="MJF80_003446"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBZ0282058.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5902..7830
                     /locus_tag="MJF80_003447"
     CDS             5902..7830
                     /locus_tag="MJF80_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBZ0282059.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3175 a         1022 c         1606 g         2287 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatatt ggggattagc caagtcggta aggcacatga ctttgactca
      541 tgtatgcgta ggttcgagtc ctgcatcccc agccaaacag tatggtctat tagctcagtc
      601 ggtagagcac ctgactttta atcagggtgt cccgcgttcg agtcgcggat agatcaccaa
      661 tggagaggtg tccgagtggt ttaaggagct ggtcttgaaa accagtgact tcgaaagggg
      721 ccgtgggttc gaatcccacc ctctccgcca aatgcccaga tagctcagtc ggtagagcag
      781 gggactgaaa atccccgtgt cggtggttcg attccgcctc tgggcaccaa aatgtggcgg
      841 tatagcttag ttggctagag cgttcggttc atacccgaaa ggtcataggt tcgactccta
      901 ttaccgctac taaaatttaa tgtgggccat tagctcagtt ggttagagcg cccggctcat
      961 aaccggtagg tctggggttc gagtccctga tggcccacca cattaaatta acttatggtg
     1021 ggtatagctc agttggttag agcgccagat tgtggctctg gaggtcgtga gttcgactct
     1081 cattatccac cccaacttat atggtctatt agctcagtcg gtagagcacc tgacttttaa
     1141 tcagggtgtc ccgcgttcga gtcgcggata gatcaccaaa aaggcggcga catagccaag
     1201 tggtaaggca gtggactgca actccttgat ccccagttcg aatctgggtg tcgcctccaa
     1261 taaaatgcgc tcatagctca actggataga gtgtctgact acgaatcaga aggttagggg
     1321 ttcgagtccc tttgggcgca ccatatgaat agcgagggat tatagctcag ctgggagagc
     1381 acctgcctta caagcagggg gtcacaggtt cgagccctgt taatcccacc agtgcaaacc
     1441 tctagtaaaa ctactagagg ttttattttt gattgaaaaa atttttatat ttaggaggta
     1501 aaaaatttgt atatagtaat aggaaatgaa atagttgata gtgaagaact taaaataatt
     1561 atagataaaa atagtgaatt taaagttgaa aaagatttaa gtaaaagcac aaaaagagaa
     1621 gatgtaatag catatcagtt aagtatagat ttaaattacc tagattctct tataaatgag
     1681 caatgtaatt tagccagtct atctgatgaa gagaaatttg atgaatatat gactttgtct
     1741 gatgaacttg cactggattt agaagagtta atgccaaaat atacaataat aaatgctaga
     1801 gcatataaat tagacgaggt atatggaatt gtaaaaataa tactagctgt agcgtatgca
     1861 gatttagggc acttaaaact ttctgatgta gtaaagaggc tatctagaca agtagattag
     1921 gatatgaata tgaactatat taaaaaaatg tataaaatct tacgtaaaga tgaaaataat
     1981 tatgtaaaca tagaaaaaaa cagtataaaa tcaagtcaat tgtagaaatt ttcacataaa
     2041 ttcatctgaa attctataaa aaatgcctta attttaatga ggtaaatcga gtttacacag
     2101 tctaaaaatt acttatattt ataaatttat cttattaatt aaggaacttt cttaaacatc
     2161 ttcaaaaata aagtatttta gtctataatc tttaatatta cacaaacaat aaaggagaag
     2221 aatagagtat gcttaaaaag tttaataaaa tactagtggc taatagaggc gaaatagcta
     2281 taagaatatt tagagcctgt tcagaattgg ggataaaaag tgttggtata tacagtaagg
     2341 aagataagta tggattgttt agaacaaaag cggatgaatc atatttgata ggagaaggta
     2401 aagggccaat agatgcatat ttagatatgg atggaataat agacctagca aagagaaaaa
     2461 aagttgatgc tatacatcca ggatatggtt ttctagcgga gaatgcagaa tttgctcgta
     2521 aatgtgaaga gaatggaatt acttttatag gaccatcatc taaggtaatg aatatgatgg
     2581 gggataagat taactctaaa aaaatcgcaa aagaagttaa tgttcaaact ataccaggag
     2641 tagaaaaagc aataagaagt actgaagaag caaaggaagt tgcaaacaaa ataggatatc
     2701 ctgttatgat aaaagcatct aatggcggtg gcggtagagg tatgagaatt gtacatcgcg
     2761 aagaagactt agagcttgaa tatgaaactg cttgtagtga atcaagaaaa gcttttggag
     2821 aagatattat atttatagaa aaatatatag ctgatccaaa acatatagaa gttcaaatat
     2881 taggtgataa ttatggaaat atagttcact tatatgaaag agattgttca gttcaaagaa
     2941 gacatcaaaa aataatagaa tatgcaccag ctttttcgtt agatgataaa gtaagaaaag
     3001 agatatgtga agatgcagtt aagttatcaa aacatgtggg ttattctaat gcgggaacat
     3061 tagaattttt agtagatgca aatggtgggc attattttat agagatgaac acaagagtac
     3121 aggttgaaca tactgtaaca gagatggtta caggaataga tatagttcaa agtcaaatac
     3181 taatagccca aggttacagc ttagatagtg aagaaattaa tataaaatca caagatgatg
     3241 tggaaataag aggttactct atccaatgta gaataactac agaagaccct aagaataaat
     3301 tcatgcctga tacaggtaaa attcaagttt atagaactgg ttcaggattt ggtataagac
     3361 tagatggagg aaatggattt acaggggcaa acataagtcc tcattatgat agtttattgg
     3421 ttaagacaat atcttgggat agaacattcc aaggagcaat aaataaaact ataagatcta
     3481 taaaagaact tagagttaga ggtgttaaaa ctaatgtagg attcctagtt aatgttttaa
     3541 ataatcctat attctcaaat ggtaaatgta gtactaagtt tatagatgaa aatccagatt
     3601 tatttgaaat aacagaaagt aaagacagag gaacaaaatt acttcaattt ataggagatg
     3661 taattgtaaa tgataatgct tgtaaagaaa aaccattatt tgatgcatta catgacccaa
     3721 gaatggataa ggatggttca aaatcagaag gaagtaaaat acttttcgat aagttaggta
     3781 aatctgcata tatagaaaag ataaaaaatg ataagaaatt actattaaca gatacaacaa
     3841 tgagagatgc acatcaatca ttattagcaa caagaataag aacatatgac ttattaaaag
     3901 ctgctaagcc aacagaaaaa tatcaaaagg atttattctc acttgaaatg tggggaggag
     3961 ctacttatga tgttgcatat agatttttaa aagaatctcc ttggcgtaga ttacaaaagc
     4021 taagagaaga aataccaagc ataatgttcc aaatgttgct tagagcatca aatggagttg
     4081 gatataaaaa ctatccagat aatgtaatag aagaatttac aaaagaatct gctagacaag
     4141 gtatagatgt atttagaata tttgattcat taaactgggt tgaaaatatg aaaccatcta
     4201 ttaatacagc tttagaaact ggaaaaattg ttgaagcaac tatgtgttat acaggtgata
     4261 tactagataa gactaaaact aaatacaatc tagaatatta tattaaaatg gcacaagaat
     4321 tagaaagctt gggagcagat ataatagcaa taaaagatat gtcaggtctt cttaaaccat
     4381 attcagctta cacactagtt aaagagttaa agaaaaatgt aaaagcacca atacatttac
     4441 acactcatga tacaagtgga aatggtgtag caacttgttt aatggcgtca gaagctggag
     4501 tagacataat tgatgctgca ttagaatcta tggcaggact tacaagtcag ccatcactta
     4561 atgctatagt agaggcactt aagaatacag agagagatac aggaatagat ttatttggat
     4621 atgatgagtt aggaaaatat tataaggatt taagaaaagt ttataataaa tttgaaagtg
     4681 atttgactaa ttcttgtgca gaaatatata attttgaaat accaggaggc caatatacaa
     4741 acttaaaacc tcaagctgat agtttaggat tagttaatag atttgatgaa gttaaagaaa
     4801 aatataaaga agctaatgaa gttgtaggag atataataaa agtaactcca tcttcaaaag
     4861 ttgtaggaga cttagctatc tttatgacta aaaataaatt agataaagat aatataatag
     4921 aagaaggtaa aaatttatca ttcccagatt ctgttgtgga ttactgtaaa ggtatgattg
     4981 gtcaaccaga aggtggaata ccaaaagatt tacaagaggt agttctaaaa ggtgaagaag
     5041 ctataacagt aagaccaggt tcattattac cagcagaaga ttttgatgaa atagctaaat
     5101 atctaaatga aaagtatgat ataaatgcta atataagaaa tgttataagt tatgctttat
     5161 atccaaaagt atatgaagat tatatcaaac atctacaaca ttataatgat atatctaaac
     5221 ttgaaagtga tgtattcttc tatggattaa ataaaaatga agaatgtgaa gtagaaatag
     5281 aagaaggtaa ggtattaaca ataagattag ttgaaattgg tgaagttaag gaaaatggat
     5341 ttagaactat aggatttgag ttaaatggaa tggttagaga agtagaaata aaagataaga
     5401 atttctctgg aaaaattaat aatgttgaaa aagctgatat gaatgaccct cttcaaattg
     5461 gagcaagcat accaggaaaa gttataaaaa taatggttaa agaagaagat gaagtaaaag
     5521 caaatcaacc attaatcgtt atagaggcta tgaagatgga aactataata gttgctaaga
     5581 ctgatggagt aattaagtca atcaaagtaa aagaagatga tatggttgaa gataaacaat
     5641 tattaatgat aatgaagtaa tttaattaaa ataaattaaa aagcactctg aaatgtttaa
     5701 taaaaaatgt tttagagtgt ttttatttat tttaattaaa tatttgttaa aaaaaagagg
     5761 acaattacct ccagatgtag aaataaagct agtaaggaaa atgttatcta aaaattctaa
     5821 atatagtaaa tattttccat aatatttctt aggaaataac agcaaagata aaatacataa
     5881 atttaaaagg tgggtgctta tatgaaggtt tatgatagta aaatgttgag aaatgtagcg
     5941 gtattaggac atagcggatg tggaaaaaca aatctaatcg aaactatagc ttacacagca
     6001 aacactaata agatacctaa attaacagac aaggtaaata tgacttatag catgggatta
     6061 atacctatag agtataatga ttataagttt aacttattgg ataccccagg atattttgac
     6121 tttagcggag acgttgtgtc atctcttaga gcaagtgatg cagcaataat agtaatagat
     6181 gcaacagcgc ctattcaagt tggtacagag aaatcattag aactaactga gagtattcct
     6241 aagataatgt ttataaataa aatagataat gaaaaggcaa gatacaaaga tgcaatagca
     6301 atgttaagag aaaaatacaa taataagata gttcctatga taagtcctat atacaaagat
     6361 aaaaactttg taaagttaca taatgttttt gaaaacatag atgatttaga aggtgaattt
     6421 aaggaacaag ctatgagtgt taaagaagca ttaatggaac ttatagcaga aactgatgac
     6481 caaatattag ataaatattt taatggagaa gaactaacta cagaagagat acaaaaaggt
     6541 attattatag gtatacaaag aggtgatatt atacctgtaa tatgtggttc aactataaat
     6601 aatataggca caaaggaaat attagataca atatcaagtt atttagaacc tatctttaca
     6661 gaggaaagca aaccatttag gggattagta tttaaaacta tggttgaccc atttgttgga
     6721 aagatgtcat atataaaaat tactgagggt gtactttcta aggataaaga tgtttttaat
     6781 ataaataaaa atgtaaaaga aaaaatagct aatatatata cattgagaaa tagtgaatta
     6841 gtagaaatag aaaaagctaa agctggtgat atagtagtta taactaaagt taattctcta
     6901 aaaacaggag acactatatc tgcagataaa gatgcagaag ctttagaaaa gatagatttt
     6961 cctaaaccac aaatatatta tgcagtaact cctaagaata aaggtgatga agaaaaagta
     7021 gcatctgtat taaataaact agtagaagag gaccctacat tacattggta tagaaataca
     7081 gagacaaaac aagcactttt aggaggtcaa ggagaacttc atataaaaac aattaaaaat
     7141 aaaatgaaag ataagtttgg tgttgatgtt gaattgaatg accttaaagt tccttataga
     7201 gaaactataa aaggaacagc tgatgtacaa ggaaaacata aaaagcaatc aggtggtcat
     7261 ggtcaatatg gagacgtaaa aataagattt gaaagatgtg aatctgattt tgaatttaca
     7321 gaagaaatat ttggtggttc tgtaccaaaa caatatatac cagcagttga aaaaggttta
     7381 aaagactcta tgcaaaaagg tatattagca ggatatcctg ttacaaacat aaaagctacg
     7441 ctttatgatg gttcatacca tgatgtagac tcttctgaaa tggcctttaa aatggcagca
     7501 agtgcagcct ttaaaaaagg tatggaagaa gcacacccaa tactactaga acctataatg
     7561 aaacttaaga taactgttcc agaagaatat atgggagatg tcatgggtga tataaataag
     7621 agaagaggaa aaatatttgg aatggaacca gatgacaaag ggaaacaaat aatatttgca
     7681 gaagctccac aagcagagac atttaaatat gcaatagatt taagagcaat gacacaaggt
     7741 agaggatact ttgaaatgga attagagaga tatggagagg ttccatcaca gtttgcagaa
     7801 aaaataattg ggctagctac tgcaaagtaa tgaacaaaat aaatagaaaa ataattaata
     7861 tatattatta aaaatatttt tagggtttta actaggtata ggtttatgtt aggtaaactt
     7921 atacctagtt tttatacatt tttgatgaat aaaaaaatat ataatacttt cttaaatatt
     7981 ttaataaaaa gaaagtattt ttttattatt atttttacaa atgtttagta ataaagatga
     8041 gaaggtatat ttaaaatata aagtatttaa agttctgaaa tctaatcatg
//
LOCUS       DAGSWI010000165         7984 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.168, whole genome shotgun sequence.
ACCESSION   DAGSWI010000165 DAGSWI010000000
VERSION     DAGSWI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.168"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            105..815
                     /locus_tag="MJF80_003448"
     CDS             105..815
                     /locus_tag="MJF80_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282060.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            809..2125
                     /locus_tag="MJF80_003449"
     CDS             809..2125
                     /locus_tag="MJF80_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBZ0282061.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            2137..2277
                     /locus_tag="MJF80_003450"
     CDS             2137..2277
                     /locus_tag="MJF80_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBZ0282062.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            2336..2917
                     /locus_tag="MJF80_003451"
     CDS             2336..2917
                     /locus_tag="MJF80_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBZ0282063.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            3064..4773
                     /locus_tag="MJF80_003452"
     CDS             3064..4773
                     /locus_tag="MJF80_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282064.1"
                     /translation="MSSIKVISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTKMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKVNSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSYMAYKINLSSKSNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            4788..5660
                     /locus_tag="MJF80_003453"
     CDS             4788..5660
                     /locus_tag="MJF80_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0282065.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLTDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            5661..6449
                     /locus_tag="MJF80_003454"
     CDS             5661..6449
                     /locus_tag="MJF80_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282066.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVFYIARKSTVLKKSK"
     gene            complement(6545..7183)
                     /locus_tag="MJF80_003455"
     CDS             complement(6545..7183)
                     /locus_tag="MJF80_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HBZ0282067.1"
                     /translation="MIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGYASDIRMQ
                     KTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLNKESKLDK
                     AVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRAKKDNRFL
                     EALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLEK"
     gene            complement(7210..7839)
                     /locus_tag="MJF80_003456"
     CDS             complement(7210..7839)
                     /locus_tag="MJF80_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBZ0282068.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         3235 a          754 c         1130 g         2865 t
ORIGIN      
        1 aaatctaaat agctatttac tacagaagta tgttaaaata tgtaaaatga atagaaattt
       61 atatattttt attaaaaata tataagaaag gatgtttaag aattgtgatt aatataggaa
      121 tatgtgatga tgaactacat tatcgaataa aaataaaaga tatattaagt gaaatattaa
      181 gttcatatcc tataaattat aacatatatg aattttcagc aggagaagag ttactaaata
      241 actatcctaa agatttagac atattaatta tggacataca aatgaaaaca ataaatggta
      301 tggatactgc taggaagata agagagtttg atcataagtt agaaataata tttgttactt
      361 catttgtaga gtttatgcaa gaaggttatg aggtaaaagc atacagatat atactaaagc
      421 caataaataa agaaaaaatt tctaaaagtg tattaccttg tataaatgag atgatgaaaa
      481 agagaaataa ttacttaact ataaatgtaa aaaattatgt agaccgaatt aaaatagatt
      541 ctattactta tatagaaaca gatagaccga atatattgat ttatacacat gatgatatgt
      601 atattactaa aatgagtata tctaaaatag aaaagatatt aaatgaatat ggatttttta
      661 gatgtcataa tagctatata gttaacttaa aattagtaga atcaatgagt ggaagtactg
      721 ttatagtaga tggaaaaagt attccaataa gtaaatatag ggttaaaggc ttgaagcttg
      781 cgataactaa tatattaggg gacatagtat gctaacaaat ttaagtgtag gattttggaa
      841 tattattaat ttagtttcag gtactataga ttggattata ttttttttag taatccactt
      901 agttggaaag aggaaaatat ctaatataag atatatattt acaacaataa ttataattat
      961 atttatggca tacataaatg tagttaaagt gtttccaaat attaagattc ttctatgtat
     1021 ggtaatagga atatcatttt atataacatc atatgaagat aaaatctata aaagtataat
     1081 agttagttta ttgttttggc taggattaat ggttgctgaa ggttctgcag taggtctagt
     1141 agtatttata aataaattaa ataatattaa cataacacaa catgaaaact tatttagaat
     1201 acaagctatt attatgtcaa aggtattttt atttataggt tttatattgt ttaagtattt
     1261 taaattatct ttagatttta aacctaaaga tatgatacta ataggaatac ctatattatc
     1321 taatatagtt agcttattac ttatatttga atataattta agagtcaata caattactac
     1381 agaaaactta tttatattta tatttgcaat tttattgatt ttatcatcaa gtataatact
     1441 tttaatagta atagggaaaa ttgtacaaga cgataaatta aatctagagt atgaacttat
     1501 aaatgaaaga ataaaaacaa actacaggaa ttatgaaaat ataaacgaag ttcatgatag
     1561 attaaaatat gtttatcatg acttgaaaaa ccatatgatt tgtataaaaa attatgacac
     1621 taaagaagaa attatatcat atataaataa cttagagttt cagataagtg aatttgaaca
     1681 ttttaaaaat actggaaata aaacattaga tttaatttta gcagaaaaaa taagtatttg
     1741 taaaaagtat gatattcaaa ttgaagataa cataaatata tctaaattaa actttattaa
     1801 aaataatgat atatgttcaa tatttgcaaa ttcactagat aatgcaattg aagcatgtat
     1861 ggatataaat aatgagatag aaaaaagaat agaagttaaa gctacatata taaataaatt
     1921 tgcaataata aaatttataa atactaaggt aaatgatatt aaacttattg ataaacgtat
     1981 tcaaactagt aaggatgatg ataagataca tggaatagga cttgctagta ttaaatatat
     2041 agtaaataaa tatggtggtg aaacaatagt taattactca gataatgagt ttatattaaa
     2101 aataatgata ccaataaaaa gttaggagag tacaaaatga aaaaaatagc gttaaactta
     2161 cttaaaaaca tctcagcatt aagttttggg atagctgtat tatcagcaaa cagtgcaagt
     2221 tcttgggtag cacatcaggc aaaagagcca caagctttac aaaaactaaa aaaataacat
     2281 taataaaagg acctcttctt attaaaggag aggttttaca tgttgaggag gaaatatgtt
     2341 taaaagatta tcatataaat ttgccaatat attagttaat aatgagattg tagaaagtga
     2401 agattttgaa atatatagat atggatttga aactttgatt tatttcatag ttaatatttt
     2461 ggtagcatta tttataggaa ttatttttga tagatttata catacagtta tatttttaag
     2521 ttgttattgc actttaagac agttcacagg aggttatcat gctaggaatt ataaagagtg
     2581 tacattaaca tttgcagtta tttatttgat aactattttt tcagctaata atatagatat
     2641 aaataaatat aagtatttat tagtattatt aatgattatt agtatactaa caatttacaa
     2701 gttagctcct ttagaacata gaaacaaacc tcttagtgaa agtgagaaaa agcattatag
     2761 aaagactgtg caaaaaatac tattcgtaat aatatgttta attattttat gtaaaatact
     2821 taatattttt cagcaatatg tgatttatgc aataatttct atatattgga ttgctatatt
     2881 aatatatatt ggaatgaagg ttaatagtga tcagtaagat tataaaacat tttctaaatt
     2941 aattaacttc actaattaat attaacttta aaaaaataat gtcaaggtaa gtatttgaac
     3001 ttattaaata tagttgaatc tattgtaggt tgtatcaaaa gactggctag gaggggcata
     3061 aatatgagta gtattaaggt tatatcagac tttataacca aaataattag aaaaaatatt
     3121 ttatttgcaa taacaattat tattataaat taccatctca tacaacgatg gacacatata
     3181 tccaatagag gaatttcatt taatgattac ttatccttga gtgtatatac ttgtatgaca
     3241 attagtattt taaccatgtt aaatgtaatt ttttatatga gaaaaagtta tgatactgta
     3301 aaaatattat gtagaaataa ttttgattat tttttaggta tttctgttgc tgtaactaga
     3361 aacaacttta tattttgttg cataccaatt atatatgcta tagcatactc aagtttaata
     3421 agaacattgt caattgtaac gattaaatca actatattta tattgatatt tgaatggttt
     3481 ttaccactta ctattttatc tatagtgata tcatttattt cagttattat aaataattta
     3541 atactaaaga ttaccttatc atcagtattt ttatatttaa catcaactaa gatgttggat
     3601 agagttattg atattaaaga accatttttt aaagcattat ttaaatcact taacatattt
     3661 gaagatcaat catatgctta ctattctgat tttgtaggga aagttaataa ttcagcatat
     3721 gttatagata aaattatacc tacactattt gcaattactt taataatagt agcatatctg
     3781 atattaaatg atattagaaa gaaacagatt aaatcattaa tagttatagt agtgtacagt
     3841 attttattta ttggattaaa ttatatatca atagatatgt caacatatag agatgaggag
     3901 cgtagttatg gagagtattt aaagaaatct aatattagtg atattaaaat aaaatcgcat
     3961 gatatgaaga ttgactttaa aagcaagtca aatataaaag atagaattgt tttattaaac
     4021 acatcaaatg aagagttaac aaatattaat atgattttag atgaaatatt tgatataaaa
     4081 tatataaaag taaattcgga aaatataaaa tttaacaaaa aaaaggattt aattgaagtt
     4141 aatttaaagg aaagtttaga gcctaatgac gaggtgacat tagatataga gtatgaaggc
     4201 tatattaatg tgttgacatc ttggaatcaa aataaatata tagctactaa agatgatatt
     4261 atgcttccac caggttcttt agcgtggtac ccaaaagtaa atcaatcaag ttatatggct
     4321 tataaaataa atttaagttc taaaagtaat gtctattcaa atttaccttt actgaataag
     4381 actagtaatt ttttaaaaaa agattatata tttgaaggaa agtcaaatga tattgcaata
     4441 tatgcaggtg agtttaaaga agaggtaaaa gatgatataa aaataatcta tccatcagat
     4501 agtaatgtaa atatggatat agaggaaaaa attaaatttt taattgaaac aagaaatgaa
     4561 atattaggtg atagcaaagc atctgtagat gctaaaaaat atgtagatga agtagataat
     4621 gttatggaat caataaaaag aaaaaaaatt aatatgatat tagttgctga cttacctagc
     4681 aatgaatttt tatatgaaga ctcaaaggga gagaaaatta gcataactat ttatcattgg
     4741 ctttcaggaa atatccttat aatagataaa taataaagga ggttaatatg ataaacaagt
     4801 tagaaataag aaatctaaat tttaattaca aaaataaaaa agctttggat aatataaatt
     4861 taaccctaac agatggagta gttgcattat taggtcctaa tggtgctgga aaatcaacat
     4921 taatgaggtt attagtgact ctttatgaaa cttcaattgg agagatagaa ttaaataata
     4981 taaagtatag caaaaacaat gagaaaatca aggcaaatgt aggatatgta cctcaagatt
     5041 ttgatatgta taataatata aatgggcaag aatacttaga atttgtagct aaaatgagag
     5101 gtgtatctaa gaatgattta aagaaacata tacaaaaagt agtatcaaaa gttaatctgg
     5161 acaagtttat aaataaaaaa ataggtacat actcaggtgg tgtaaagaga cgactaggta
     5221 tagcacaggc tttaataggt gattctaagt taatagttat ggacgaacct acagttggtc
     5281 ttgatccaga gcaaaggaat gaatttagaa gattgttacc agaaataagt aaaaattcta
     5341 tagtattaat ttcaactcac atagtagaag acatacaatt taactgtgac aaattaatta
     5401 tattaaatca aggcaaaata ttatatgatg gtacaattaa taagtttatt gatatggttg
     5461 aggtgtattc ggtagtagtt tctaatgatg aatttgaata tttagaacgt aatattgata
     5521 ttattgatta taggagagtt aaagatggtg tgcaagtaaa gtatataggt aaaaatacag
     5581 agaaacttaa agatagctta aggatagata caactttaca agatgcttat ataaactttt
     5641 tacataagga gttaaaataa atgtgtatat tggagttaat aaaatttgag cttaaaaaaa
     5701 tagagcttaa acatgtgtta atatttgtaa ttatttctat cttatgcata tttattacag
     5761 gcatattaga ttataatgag ttaatatttt ataatgatgt acctataatg gatacatatt
     5821 ctatagcagg atggttagga attataaaga tatcagatgt ttttatacca ataatgtcag
     5881 caatcacaat aatttatgtg ttttcaaaag attattcggg taatataaac gagatactta
     5941 ctttatataa taaaaaaaaa tataatcaat ttatagtatt tagatggtgt gttatattag
     6001 ctatatatac tgtattatta ttaatttcaa ttacaatagt atatacattt acgtcagtga
     6061 aaggttttaa catagacaaa atagattttg tttatagtgt aaagccaata gatgtgtttt
     6121 taaagacatt tcctacatta ttatggtata caacatttcc actaatgata ttaacattaa
     6181 caaaaaatag atttacaaca atagcaatat taattacata tacatttgct gatatatttt
     6241 ttattttaca tatgtaccca tttgttagta tgattaatgt aaatggattt tatattcaaa
     6301 agatgtttta tatgcctaac aatatagatg ttagatttaa tgaattaaag aattatttct
     6361 ttataaatag aggttctcta gtagttattt ctataataat agttttttat atagcaagaa
     6421 aatcaacggt tttaaaaaag agtaaatagt ttaatatatt ttttatattg ttatatttat
     6481 atagaatagt gactttaaaa aatttaaagc cactattttt taatatgttt tatatatata
     6541 actattattt ttctaggttt tttaatattt cgtaatagtg tgttgtggct atttcacttg
     6601 actcgccttt tttacaaaaa gataatttgg atagttttgg aactacacgt tcaacgacaa
     6661 ttttattacc tatcatttta gattcaagta tcttcaatgc ttctaaaaat ctattatcct
     6721 tttttgcacg attataaaat gataaaacgt agacataaag gaataggtta taattacgga
     6781 aaggatattc aacttgcata aataatgtgc caattccata gtgacaaggg ccgattggtt
     6841 ttttaatagt ccaatggtca agtagaaaat ctacagcttt gtcaagtttt gattctttat
     6901 ttagataatt actaaaacgg aatgcattta atatattaag agttggtagt ggatttgagt
     6961 attcagtttc tggacctcga ccaaaactaa atttattgca acgccaccca ccatcagtat
     7021 actgtgtatc taaaaaatgt tgaaacgttt tttgcattct aatgtcagat gcatatccca
     7081 tatgacagag tacgttagca gcatttgcag tatgacaagg gtaaatacct ccatttggat
     7141 atagtttgaa acgtccatct tctcgccatg tgctaaatat caaaaacttt attataatat
     7201 tatttatatt tataattgat ttattttatt taaaattgca tttatagctg tatcaggaac
     7261 aactgctaaa tcttctgcat tgaagtaata aactatatta gaatcaaata atatatttga
     7321 atttggagga aattcatcat ctccaaacca aagtataaat atcatagatg tattacccat
     7381 atatgtaaac ctataagcta aatctcccat gtttactttt tcagctccaa ttttctccat
     7441 aactaactta tatttgtcta tttgagatgt aaaaacttct gaaagcctat atagagtcct
     7501 gtttaaaaaa tttggataat aaacattgcc tcctggtatt tctttaaaag ttacatattt
     7561 attagttcta ggaatacctt tagcattgat taaatatcta actatcaaga ttttaagctc
     7621 aaagttattg caaaggctac cattactatt ccaaacttct ccacttggat agtctataat
     7681 atattgtcta tcaaaaaaat tgagtgtgaa tctatttaaa tcattattga aaggtatatt
     7741 acttaaatta ctaattgtat gagggtctaa ttctttgagt ttttttcgaa catagtcaaa
     7801 tggaacatta taattatttt ttgttatctt ttcatccata attttttcct ccaatttaaa
     7861 caattaaaag tattaaaaag ttttcttatt attaatttta ttatatattc aaaatattac
     7921 aataataaat tgttaaataa atttttatat ttaagtaaat acgtgtaaaa tgaatataaa
     7981 gttt
//
LOCUS       DAGSWI010000166         7619 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.169, whole genome shotgun sequence.
ACCESSION   DAGSWI010000166 DAGSWI010000000
VERSION     DAGSWI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.169"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            11..1069
                     /locus_tag="MJF80_003457"
     CDS             11..1069
                     /locus_tag="MJF80_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBZ0282069.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGSYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1173..2357
                     /locus_tag="MJF80_003458"
     CDS             1173..2357
                     /locus_tag="MJF80_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBZ0282070.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2564..3277
                     /gene="rpsB"
                     /locus_tag="MJF80_003459"
     CDS             2564..3277
                     /gene="rpsB"
                     /locus_tag="MJF80_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBZ0282071.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3356..4267
                     /locus_tag="MJF80_003460"
     CDS             3356..4267
                     /locus_tag="MJF80_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBZ0282072.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4352..5056
                     /locus_tag="MJF80_003461"
     CDS             4352..5056
                     /locus_tag="MJF80_003461"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBZ0282073.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5082..5639
                     /gene="frr"
                     /locus_tag="MJF80_003462"
     CDS             5082..5639
                     /gene="frr"
                     /locus_tag="MJF80_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBZ0282074.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            5642..5845
                     /locus_tag="MJF80_003463"
     CDS             5642..5845
                     /locus_tag="MJF80_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282075.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5874..6608
                     /locus_tag="MJF80_003464"
     CDS             5874..6608
                     /locus_tag="MJF80_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBZ0282076.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6621..7400
                     /locus_tag="MJF80_003465"
     CDS             6621..7400
                     /locus_tag="MJF80_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBZ0282077.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         3138 a          785 c         1448 g         2248 t
ORIGIN      
        1 atatatattt atgaattgta aatggatatc taaaatagat gagagaaaaa aatgtcatag
       61 agaagcagat tcttctggat attgtatctt tcataaagaa aataagtctg atgaagagat
      121 acagttgatg atggatactc ttcataaaga agagataagt gaatttaatg gatttgtttt
      181 tgaaaatgaa tttaatgcgg aagaaatatt aacttacaat tataaaatac tggatttttc
      241 tgaatccata tttaaacaaa aagcaaattt taaaaaatat atttttaaaa agaatattat
      301 ttttaactat acagaattta gagataaagt tttatttaat ggttgtgtat tcttagaaaa
      361 ctgtgatttt aatagaacca tatttagtaa acactatata aatgaccgta tatttgaaaa
      421 agtgaaattt aaaggacctg atttggtagt taataaagta gaaaattttc cacgaatgga
      481 tggaataatt tttagcatgt gtacaaaatt tgtgttaaag aatgttgaat atggaaaaag
      541 tgagtatgag catggcaaga taaattatag aatagctaga aatcaagcaa caaaaattgg
      601 agaatatgag atgataggat cttactatta taaggaaaga atttatagta gtaaaataat
      661 gaaacgttct aattatccta ctttcagtga ttatttagtt gaaaaatttt ttgaccaaat
      721 agcacgttac acaacaggtt atggagaaaa gccatggaat atattattag ttattatagc
      781 aataataagt gtttttgctt tattatactt atttgtaggc atagaaagtt caaatagcac
      841 acttgtagct ttagatataa acaatatagg tgattactca ctaagtgaga ttttcaaaat
      901 gtatatggat ttatggtatt ttagcatggc tacatttagt acagtaggat atggagatat
      961 ggttgctaca agtttgattg ggaaagcatt ggcaggtata gaagtatttt ttggagttac
     1021 aataggagca atttgggcat ctgtgattat taaaagaatg ataaggtaat atttgtgtaa
     1081 atatggatag gtattaatta ctagatataa attggactaa atagggaaaa tttaaatata
     1141 aatatactta ataatccaag aaggtgaaaa atttgaaaaa tgcactaaaa aaaattatat
     1201 cattagccat gattctagtt tttataatac caaattattc atatgcagat gataaatctg
     1261 tagacaagta ttcaaaaaca tctatactta tagaccaaga aacaggtaga gttttatata
     1321 gtaaagaacc tgatatgaag gtaccattag ctagtttaag taagatgatg acttttttac
     1381 ttgcaataga agctattgaa aataaagaag taaaagaaac tgataaagta aaaatagaca
     1441 aatctattgc atcagtaaaa gggtcaagtt ataaacttaa agatggtgaa gaagtgccac
     1501 ttatagagct tatgagaggt cttatgattg tatcaggaaa tgatgcggca attgctatag
     1561 caaaacatat ttgtaaaacg aaagaagcat ttgtagatag aatgaataaa aaagcaaaag
     1621 aaataggaat gagtaataca cattttctaa atccaaatgg tcttccaata tacgatttaa
     1681 gcaaccccaa aaaaccagca aaagaaaata tctctactgc aaaagatata gctattcttg
     1741 gaaaatacat gtttgaccat tatgaaaaac aagtaacagc tataactgat atggagacat
     1801 atacaaattc agatagaagt tttgaaaaaa gcaatacaaa tgcattatta agaattatac
     1861 cagaagtaga tggtataaaa actggatata ctggcaatgc tggatattgc ttatcatttt
     1921 ctatggttgt aaataaaaat gataaaaatg agaaaaaacg tagagtaata ggggttgtat
     1981 taggtactaa ccataaaaat aaaagaacct ctgcttcatt agctctactg aaatatggaa
     2041 aagaaaattt caatatgaaa aaagtaattg ataaagatag ctttattggt aagaaatata
     2101 taaaaggaat gaaagagtta gaggttacat taaaagcaaa agatgaactt tatacaatat
     2161 taaaagatga tgaaagtata aaatcagaag ttaagattaa aaatatagag taccctgtta
     2221 aaaaaggtga tactatgggt attataaaat attatactaa ttcaggtgac ttacttggaa
     2281 gtgtagatat agtaagtaat aatagtatag ataacgtatc attaaaaaca aaattaaaaa
     2341 tgaaatttgc aaattaaatt caaaaaatgg tataaaatac acaaaaaaat ataaaaatag
     2401 aaaatatgga aaaaatattg ttatttcttc acctctgtga tatactacta gaggtgaaaa
     2461 aataaaataa atcacacaca gcattgggat ccttaaaagg tgcctattac atggtttttt
     2521 agggagaaga tcaagctgga ggtataaaac caggaggtaa gatatgtcag taatatcaat
     2581 gaaacaatta ttagaagcag gtgttcactt tggtcaccaa acaagaagat ggaaccctaa
     2641 aatggctaag tatatattca cagagagaaa tggaatatat ataatcgact tacaaaaaac
     2701 agttaaaaaa gttgaagaag cttacaagtt cactaaagaa gtagctgaaa caggaaagcc
     2761 aatcttattc gtaggaacta aaaaacaagc tcaagatgcg ataaaagatg aagctgaaag
     2821 atgtggaatg tactttgtta atgaaagatg gttaggtgga atgttaacaa accacaaaac
     2881 tataaaaaca agaataaata aattaagaga attagaaaaa atggaagaag aaggcgtatt
     2941 taatgttctt cctaaaaaag aagttataaa attaagagct gaaaaagaaa aactagaaaa
     3001 atacttaggt ggaataaaag acatgcctga attaccaggt gcaatgttcg tagttgaccc
     3061 aagaaaagaa aacatagcta ttcaagaagc tcatagatta ggtataccag tagttggtat
     3121 agttgatact aactgtgacc cagaacaatt agactttgct ataccaggaa atgatgacgc
     3181 tataagagcc gtaaaattaa taactggtgc tatggcaaca gctgtaattg aaggtagaca
     3241 aggtgctgag gaagaagtag ctgaagacca agaataataa tttgacaatt aaatggtaag
     3301 ggtcgccctt accatttata atatggaata gaataagtag gaggaaactt aaaaaatggc
     3361 taatataact gctcaaatgg taaaagagtt aagagaaagt acaggcgcag gaatgatgga
     3421 ctgcaagaaa gctttacaag aagctgaagg aaacatggaa aaagcagtag atttattaag
     3481 agaaaaagga ttatcaaaag ctgctaaaaa agctggtaga gttgctgctg aaggtctagt
     3541 tgcaatagaa atgaatgatg ataatacagt tgcttctatg gtagaagtta actcagagac
     3601 agatttcgtt gctaaaaacg aagacttcaa agtgttcgtt aaagatgctg cttgtatggc
     3661 attagctact gataaagaag atatagcttc tttattaggt gaaactcata aagagggaat
     3721 aactttacaa gaagttttaa ataatagagt tgcaaaaata ggtgagaaat tagactttag
     3781 aagatttgct aaagttgtaa ctaatggaca agttgctgga tacattcacg gtggtggaaa
     3841 aataggtgtt cttgttgaaa tggaaacaga agctagagat gctaaagttt tagaattagg
     3901 taaagatgta gctatgcaag ttgctgctat gaatcctaag tatgtttcaa gagatgaagt
     3961 agatgctgaa tacatagcac atgaaacaga agtattaact caacaagctt taaatgaagg
     4021 aaaaccagct aatatagttg aaaaaatggt taaaggaaga ttagaaaaag aattaaaaga
     4081 ggtttgttta ttagagcaaa ctttcgttaa aaatccagat ataactgtta aacaattagt
     4141 tgctgatgtt gctaaggcag taggttctga tataaaagtt gttaaagttg tgagattcga
     4201 agttggtgaa ggtatacaaa agagagaaga aaactttgca gaagaagttg ctaagcaact
     4261 taagtaataa ccaaagagag aacacgcagg tgttctcttt tttaaaaaat agaaaaaatt
     4321 ggatatgaag gattgaaata ggaggtttct gatggataaa ccaatgtaca aaagggtatt
     4381 gttaaaatta agtggagaag cattagcagg agaaagaggt tttggtataa ataatgatgt
     4441 agttaatgat attgctattg ctataaaaaa aatacaagaa ataggtgttg aagtagctgt
     4501 agtagtaggt ggaggaaact tctggagagg cagaaccagt gaaggaatgg acagaactac
     4561 tgctgactat ataggaatgt tagctacagt tatgaatgca atggcgctac aggatgcact
     4621 agaaaatata gatgttgcta caagagttca aactgctata gacatgagac aaattgcaga
     4681 accatatata agaagaagag cagttagaca cttagaaaag gaaagagttg ttatatttgg
     4741 tgcaggaaca ggaaaccctt actttacaac agatacaact gctgctttac gtgctgctga
     4801 gatggaagca gaagttatat tgttagctaa aaatgttgat gcagtatatg acaaggatcc
     4861 aaaagtacat gcagatgcta agaaatttac agaattaagt tacatggaag ttatacagaa
     4921 agaattaaaa gtaatggatt ctacagctac atctttatgt atggataata aaattccaat
     4981 aaaggtattt gagcttacaa cagagaatat aataagagca gtaaaaggcg aaaatatagg
     5041 aactactgta aaataatgaa gaatgaagga ggagtaaaat tatgaaactg gaaatacata
     5101 agcaattaga agagaaaatg aatgggacaa ttgatgcttt aaagtttgaa tttggaacta
     5161 taagagcagg aagagctaat gcacaaatgc tagacaaaat aagagttgat tattatggaa
     5221 ctccaactcc aataaatcaa ataggtgcta tatctgtacc agaaccaaga atattaatga
     5281 tatctccttg ggataaatct gctatgcatg aaatagaaaa agcgatagct aactcagact
     5341 taggattaaa tccatcaaat gatggtgagg ttataagact ttctgtacct gctttaacag
     5401 aagaaagaag aaaagaatta gctaaaaaag ctagtaaagc tgcagaagaa tttaaagtta
     5461 gaataagaaa tgaaagaaga gacgccaatg agaaaattaa aaagatggaa aaaggcggag
     5521 aattaactga agatgaatta aaaaaagcac aagatgaagt tcaaaaaatg actgataaat
     5581 ttataaaaga aatagataca ttattatcta aaaaagaaaa ggacataatg gaggtataat
     5641 attgaaaaaa tattataacc ttttaggtct tcatgtagat gaggtaaaac aatattttga
     5701 tgagcaaaat ataaattata ccgtaaaatc tattgaaggt aaaaaggata aagaaaaact
     5761 tgtagttcct agagttataa aaatatctga gataggtgat agtgtagaac tgataatcac
     5821 atacttttct gactccttag tttaaggagt cagaacaata ttggaggcaa aatatgaata
     5881 acaacattat atatgatata gacttaaata acatacctac tcatattgct attataatgg
     5941 atggaaatgg aagatgggca aaagctagat ttcttccaag aactgcagga cataaggctg
     6001 gagtagaaac tataagagat atagtaaaag aatgctcaaa attaggtgtt aagcacttaa
     6061 cattatatgc attttcaaca gaaaactgga aaagaccgaa acttgaagtt gacacactta
     6121 tgaacttatt atctacatac ttaagaaatg aaatagctga acttcaccaa aacaatgtaa
     6181 aagtaactgc tataggagat ataagtgctt taccaaaaac ttgtataaga gaattaaact
     6241 cagctaagga actgacaaaa aacaatacag gagttaactt aaatttagca ttaaactacg
     6301 gaagtagagc agatataaaa aatgctctta tagatatagt aaaaaattgt gaaagtggta
     6361 aaatagatat aaacaatatt gatgaagata ttataaaaaa ctatttaagc acaaagtcta
     6421 taccagatcc agacttggta ataagaacaa gtggtgaaca aagattgagt aactttttac
     6481 tttgggaagt tgcttattct gagttttact ttactgatat acattggcct gattttaaaa
     6541 aagaagaact acagaaggct atatacgtat atcaaagaag ggatagaaga tttggaggac
     6601 ttaagtaagg agattatttt atgcttacaa gaattattgc ttcactagct ctggttccat
     6661 tatttctatt tgttgtatat ggaggtattc ctctatatat agctgagaca gcaatagttt
     6721 atattgcatt gcatgagttt tataaggcat ttaaaataaa agatgtacat ccaatattca
     6781 ttataggata tttattttct atttatttag ctgtaaaaaa tatttttaat ttaccattag
     6841 aatatactta tgcagtaatt tttattttat ttttagcaag tataatttat atgttgatgg
     6901 gcaaaaacaa tgttatagat gtttcgatta cgtttttagg agttttttat ataggagtat
     6961 ttttagattt tataattata actataaatg gatttgaaaa aggtagtata tatgtatggc
     7021 ttatatttgt gatatctttt atgacagata tatttgcata ttttagtggt tatttacttg
     7081 gaaaacataa gctaattcct aaagttagtc ctaaaaagac tatagaagga gctattggag
     7141 gaataatagg aagtacactt tgttgtattt tatttggata tttatttggc attgatttgc
     7201 ttcaattagc gataatagga agtataggaa gtgtaatagc tcaattagga gatttatttg
     7261 catcttctat aaagagatat gtagggataa aggattatgg aaagataata cctggtcatg
     7321 ggggtatatt agatagattt gatagtgtta tattagttgc accttttgta tatagtgcga
     7381 ttaaattttt tattagataa agattggttg gatttaaaag agcaatctat aattgctagt
     7441 aatgaaaata gctatgtaac ttttaaatgc agagatattc tgcagttatt agttacatgg
     7501 ctattttcat tgttcaaaaa tttagaagac cttggggaat tgtataagaa taggtattac
     7561 tttataatag tatactatat ttgtagtgag tatatttttt atactataac tatcattaa
//
LOCUS       DAGSWI010000167         7615 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.170, whole genome shotgun sequence.
ACCESSION   DAGSWI010000167 DAGSWI010000000
VERSION     DAGSWI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.170"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..622
                     /locus_tag="MJF80_003466"
     CDS             <1..622
                     /locus_tag="MJF80_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBZ0282078.1"
                     /translation="TTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSE
                     KIDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTN
                     ILESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISN
                     KSYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(737..1291)
                     /locus_tag="MJF80_003467"
     CDS             complement(737..1291)
                     /locus_tag="MJF80_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBZ0282079.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTHKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            1487..2689
                     /locus_tag="MJF80_003468"
     CDS             1487..2689
                     /locus_tag="MJF80_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBZ0282080.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            2746..3726
                     /locus_tag="MJF80_003469"
     CDS             2746..3726
                     /locus_tag="MJF80_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBZ0282081.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            3974..4927
                     /locus_tag="MJF80_003470"
     CDS             3974..4927
                     /locus_tag="MJF80_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBZ0282082.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            4917..5873
                     /locus_tag="MJF80_003471"
     CDS             4917..5873
                     /locus_tag="MJF80_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBZ0282083.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            5870..6625
                     /locus_tag="MJF80_003472"
     CDS             5870..6625
                     /locus_tag="MJF80_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0282084.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            6717..>7615
                     /locus_tag="MJF80_003473"
     CDS             6717..>7615
                     /locus_tag="MJF80_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBZ0282085.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSGNSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDGKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTW"
BASE COUNT         2948 a          788 c         1282 g         2597 t
ORIGIN      
        1 tacaactact agaacagtgg agcaatttaa gcgtatagaa tttaataaat atgggattta
       61 ttttccttgg agtataactt caaatggagg agttatactt aaagataatg aaatattgaa
      121 atcatggagt gaaaaaatag ataaactaaa atctgattat gagcccattg aaagtatgat
      181 tcataaattt aaagactatt tgaatgtaga tggaataact aattttaaag ttgcagaaga
      241 tacatttttt tatatagttg ttgatttatc aagatttaat ttggattcaa tcaaagaata
      301 tacaaatata ttggaatcaa aaaattggaa gttttatgtg agtggcagaa aaatatattt
      361 tataccaaaa gaaatttcaa aagaaaatgc tataaaatac ttgactaaag aattaggaat
      421 agaatatttt tatgcagttg gagattctat tatggactat ggtatgctta atatctcaaa
      481 taaatcctat gtattgaagc atggtgatat aaataaaaat gaaatagaaa attcatttat
      541 atcaagttct ttcaatggaa tgagtggaac tgaagagata ctatctaaca ttttagatga
      601 gaactgccta cttaatatat aatgactgat atataaataa taaactagaa aaatctgaaa
      661 taaattatag taaagtttat catactaatt tatttcagat ttttatcaat ttatatttaa
      721 gtaagaactc aatatgttaa aatgaaggtg gagtcattgc ccagactgag atacatacct
      781 catcatcata attttcatac acatgtggta tagtactgtt aaaataaaaa ctatctcctt
      841 cctccaatag atattcctta tttccaagtg ttatcttcat tttacctttt attatatagc
      901 cacattcttc tccttcgtgt gtcacaaatt cactgttttt gtgtgtatga ccatctagtg
      961 taacaagtat aaattctatt tttttcccag ataaattagg aacaagaagt tcgtaaaatt
     1021 ttgtagaatc attagttact attttttttc gattattttt ctttactact atatcagagt
     1081 cttctcttcc aatctcttca aaaaaataac ctatatttat atcaagagct tttgatattt
     1141 tccacattgc tacaatagat ggaacaacca tattacgctc aatttgactt attaagcctg
     1201 tacttaaatc tgaatttttt gctaaagtag agatacttat atttttagat tttctaagag
     1261 atctaatttt gcttccaaca tctatattca ttagatacaa accccctcag acatgattat
     1321 aacatataat ttataaagat taataaaata aaaaaaaagt tttacaatac aagtattttt
     1381 tatgctatat tgaattttct gaaaagatgc attataatag tattaatatt caatatattg
     1441 aatattgata acgatatttt aatttaatga aatggaggta attaagatga agagttttgt
     1501 aagtcctgat aatatttggg tattatgggc agttgtaact ggatgggcag catttagtat
     1561 ttatttggaa caaaagtaca attgggcatc aaaagtgtcg ggagcaataa tagctcttgt
     1621 aggagcaatg ttattatcaa atctaaatat tataccagta gagtctgttg tatatgacca
     1681 agtatgggga tatgttgttc ctttagccat agctttattg ttatatcaat gtaacattaa
     1741 aaagatatgg aaagaaagtg gaagattgct cattatattt cttgtaggtt ctgtaggaac
     1801 tgtacttgga gctatgattg gatttttagc attaaaaaat gtagtaccag atttaaatat
     1861 tgttgcagct atgatgacag gttcttatat aggtggaaat gtaaactttg ctgctatgtc
     1921 aggggcgttt gatgcgccag gagaattagt atcagcaaca gttgtagcag ataatctttt
     1981 aatggcatta tatttctttg tcttaatagc tataccatca attggatttt ttagaaaaca
     2041 ttttaaacat ccacatgtag atgaaatgga aagtataggt ataactgaag gagaaacagt
     2101 agcagcaaat tattggggaa gaaaagaaat atctttaaaa gatatagcaa tagccattgg
     2161 tagcgctttt ataattgtcg cagtatctgt agaactatca acttggttta aagctataat
     2221 accattatca aatccttttt tagcaatgtt aaatacttta tttggaaatc aatatctaat
     2281 aataactact ttgactatgt tgtttgccac atttatgcct aatttctttg gtgatataaa
     2341 aggagcacag gaattgggta catttttaat atatatattc tttgttgtag taggtgttcc
     2401 tgcttcaata acattaataa ttcaaaaatc accattacta cttttatact gcggaataat
     2461 ggtactaata aacatgctag ttacatttat agttgcaaaa atatttaagt ttagtttaga
     2521 agaagctatt cttgcatcta atgcaaatat tggtggtcct acaacagcag cagctatggc
     2581 aatatcaaaa ggatggtcta agctagtagg gccaatactt atagttggaa catttggata
     2641 catagtcggt aattattttg gtcttttagt aggaaatatt ttgatataat tttacgtgaa
     2701 aaaaagttgt tttttagaaa gatttttatt taattggagg ttgttatgat tttcgatgga
     2761 cattcagata cttgggcaga tgtaacaata agaaggctta acggggaaaa agatgtattt
     2821 agaagaatcc atcttgataa ttttaaaaga gcaaatatag aaggagctat ttttgtaata
     2881 tggatagacc caccatatga taaagagcct gaaaaaagag ttaaacaaat tataagctct
     2941 ataaaagatg aaattgaaga tatgaaagat gttgtaaaca tagtaaaaaa atattcagat
     3001 atagataaag gtataagtga aaacaaaata aatattttaa ttggtcttga aggattaagc
     3061 catattggaa gaaatataga tttactagat tatttttata aagaagttgg tgcaagacat
     3121 gcatcattaa cttggaatga acagaatgaa cttgctacag gatgtttagg ggatgaaaat
     3181 cgtggtttaa ctgaaatagg taaaaaagca gtcaataaaa tggaaaaatt gggcatgata
     3241 gtagatgtgt cacatttaaa tgaaaaatca ttttgggatt taatgtctgt tgcaagtaag
     3301 ccagttatag cttctcattc taatgctagg agtttatgtg atgctaaaag aaatcttaca
     3361 gataatcaat taaaagaaat ttcaaataca ggtggattag ttggaataaa tagttttaga
     3421 ggatttacta atagtaatcc taagaagcaa gatttaatac atttggttga ccatatagac
     3481 tatatggtaa atcttatagg tatagaacat gtatcttttg gttttgattt tagtgattac
     3541 ttagggaaag agactatagg ttcttttact ggaaatgcta gtgtgccaag tgtagatggt
     3601 cttgaaagca cacatacagc acagaacata gtagtagagc ttgaaaatag aggttataaa
     3661 aagaaagata tagagttaat ctctagtggt aatttttata ggctctttaa agatatacta
     3721 aaataatata ataaagaact taaatttata aaatagtata tttatacaaa taatatctac
     3781 atatttaaat tggcgttcat aatttattaa attaaatttt aatgttacta gaagtttcaa
     3841 ttctagtaac attttttatg ttaaaaacag atatagatat atattgacaa tgataattat
     3901 tattgataaa ctataaaagt gaaaatgata ataattttca ttttaattaa taaaagattt
     3961 ttaaggaggg agaatgaaga aaaggtattt agtcatagga ataataattt tgtcttattt
     4021 ttctttattt ataggagctg aagatataaa tataatgcat atatttgcca aaaatcagca
     4081 taaacttatg atatttatca tgagtagaat accaagatta ataagtatat tgattgctgg
     4141 tgtaggaatg agcatagcag gtcttataat gcaacagata agtaagaata aatttgtatc
     4201 acctacaaca ggtgctacaa tagatgcagc acaatttggg atagtaatat gtatgctttt
     4261 agtaccaact gcctctattt ttacaaagac aataatagca tttgtatttt ctcttgtagg
     4321 gacttttacg tttatgaaaa taatagggaa gttacaattt aaaaatataa tctttgtacc
     4381 attagtaggt ataatgtttg gaaatataat aggttctatg acggatttta tcgcgtataa
     4441 atacgatttg agtcaaaatg ttagttcttg gatgcaaggt gacttttcta tgatacttaa
     4501 aggaaattat gagatattat atatcacaat accccttata atattagcat atatttatgc
     4561 aaataaattt actgttgttg gtatgggtat ggattttgcg acaaacttag gtcttagtta
     4621 caaaagaatt gtcaatatag gtctgataat agtagcactt gttacagtat gtgtagtcgt
     4681 tacagctggt aatatccctt ttataggtct aatagttccc aatatcgtat ccttgtatat
     4741 gggtgataat ataagagcaa gcatatggta tactggttta cttggagcga tatttgtact
     4801 tatatgtgat atttttggaa gaattattat ttatccatat gaaatatcta tagggcttac
     4861 agttggagtt ataggaagta tactattcct ttatttaata ttaaggagga atgtaaatga
     4921 agcttagtct caatcaaaaa atttactttg taagtatatt actattcata tcaatagcac
     4981 tttttataat ttatggaata gacatgaatc atttagaata tgcattatca caaagaatac
     5041 caaaaatatt tgctatggta ttaggtggag gatgtatagc atttactact gtagtgtttc
     5101 aaacaattac aaacaatcaa atattaacac caagtgtact aggtttagat tcattgtatg
     5161 taatgattca aactataata gtattcatat ttggttcaag cagtagtctg attataaatg
     5221 aaaattataa ttttattatt aatgtagcta tgatgattgg agcttcattg ttattatata
     5281 agacgttatt tgaaaaaaat aaaaataata ttttcttttt aatcctagta ggaatgatat
     5341 ttggaacctt atttaaaagt gcaacaacat ttattcaggt aatgatagac cctaatgaat
     5401 ttttagcttt acaaactagt ataatggcaa gtttaaacaa tataaacact aatgtattgt
     5461 taatagcatt tataataatt attgccataa taccttttat atatgatgaa ataaagtatt
     5521 tagatgtatt gtcgttaggt aaagaacaag caattaattt aggtgtagat tttgataaag
     5581 cagttaaaaa aatgataata ttaatagcaa ttttagtctc aatatcaaca gcacttattg
     5641 gacctatgac atttttagga cttttacttg ctaatataac aagagaaatt tttaaaacat
     5701 ataagcatac atacttgatt tcgggttcta tgttgatagg gatgattaca ttgataatag
     5761 gtcaattttt tatacaacat gtatttaagt ttgatacaac tttaagtgta gtaataaatt
     5821 ttataggagg aatttacttt attcaattac tattaaaggg ggcaaataga tgatagagat
     5881 aaaaaatata tttaaaagat ataagaataa aaatgttgta gatgatgtct cttttagtat
     5941 agaaaaggga aagataacat cttttatagg accaaatgga gctggaaaaa gtacagtact
     6001 atcaatagtt acaagactta taggaggtga tggtggagaa gtaataatag aaggaaaaag
     6061 cttgactaat tatagcaata aagaattagc aaaaaaaata gctattctta aacaatctaa
     6121 caatataact ttaaagctta caataagaga attagttgga tttggaagat ttccatatag
     6181 tgaaggtaat ctaactaaag aggatgaaaa ttatattgat gaagcaatag aatatatgaa
     6241 gttgactgat atacaacata agtatttaga tgaattgagt ggaggtcaaa gacaaagagc
     6301 ttatatagca atggtaatag ctcaagatac agagtacata ttacttgatg aaccactgaa
     6361 caatttggat atgaaccatt ccgtacagat gatgaaggta cttagaagtc tttgtgatga
     6421 gttagataag actatagtat tagtaatgca tgatataaat tttgcatcat gttattcaga
     6481 taatatagtt gcattaaaaa atggaaaagt taaaaaagta ggtcgtacag atgagatagt
     6541 aaatgaaaaa gttttagaag atatttatga gatgaatttt aacattaaga atataaatgg
     6601 aaatcgaatc tgtatttatt tttaaaatat tgtatatgaa aataaacttg ctatatgatt
     6661 ttttataaag tttttaaatt aaaataaaga ttaataggag agaggaaaat attgatatga
     6721 ataaaaaagc agcaatagta gcagcagtag ctattatagg attagtgaca gtgtttgcat
     6781 taggtggaag taaaaagaat gaatcaaaaa catcaggaaa ttcaaataat accatgaaga
     6841 taactcataa cttgggagaa acagatgtaa aattaaatcc taaaaaagta gtggtatttg
     6901 attattcggc tttagataca atggatgcat taggtgtagc tgagaattta gtaggacttc
     6961 caaaagcgag tttaccagca tccttagaaa aatataagga tggcaagtat gcagatttag
     7021 gaggattaaa agaaccagat ttagaaggta taaagtcggc aaatccagat ttaattataa
     7081 taaatggaag acaagaagat ttttatgagc aattgtcaaa aatagcacca acaataagta
     7141 caagtaaaga tgataagaag tacttagaat ctgttaaaaa taatatagac aaaatagcaa
     7201 aaatatttgg agtagaagaa aaagcaaatc aagaatttag taaaattgaa aagaaaatag
     7261 aaactttgaa taaaaaagta acagataaaa atttaaatgc tttaactata atggtaaatg
     7321 aaggtaattt aagtgtattt ggtgaagaat caagatttag tatattatac aatagttttg
     7381 gatttgaaaa taaagataaa aatataaaag aatcaagtca tggacaaaat attacttttg
     7441 aatatatagc taaacaaaat ccagaagtta tgttcgttat agatagagga atagctactg
     7501 gcagtgatgt aaaagaaagc tcaactgcaa aatctgtttt gaacaatgat ataataaaat
     7561 ctatggatgc atataagaat gataatataa tatatttaga ttcaccaaca tggta
//
LOCUS       DAGSWI010000168         7591 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.171, whole genome shotgun sequence.
ACCESSION   DAGSWI010000168 DAGSWI010000000
VERSION     DAGSWI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.171"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..1653
                     /locus_tag="MJF80_003474"
     CDS             <1..1653
                     /locus_tag="MJF80_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBZ0282086.1"
                     /translation="ILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSK
                     NNKIHKNKALYINDVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDY
                     QINGLNWFKVLDYYKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKN
                     EFEKFAPDIKVLLIHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCIL
                     DEAQNIKNPVALVTESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKF
                     QELFINKEDNVKNLKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYS
                     KDIQDKMKDKNLKKDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSI
                     NENHKILLFSQFTSVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVF
                     LISLKAGGTGLNLTSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTI
                     EEKIIKLQESKKELINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(1938..2702)
                     /locus_tag="MJF80_003475"
     CDS             complement(1938..2702)
                     /locus_tag="MJF80_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBZ0282087.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(2858..3091)
                     /locus_tag="MJF80_003476"
     CDS             complement(2858..3091)
                     /locus_tag="MJF80_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282088.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(3165..4658)
                     /locus_tag="MJF80_003477"
     CDS             complement(3165..4658)
                     /locus_tag="MJF80_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBZ0282089.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            complement(4986..5528)
                     /locus_tag="MJF80_003478"
     CDS             complement(4986..5528)
                     /locus_tag="MJF80_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBZ0282090.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            complement(5818..6765)
                     /locus_tag="MJF80_003479"
     CDS             complement(5818..6765)
                     /locus_tag="MJF80_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282091.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGYIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            complement(6793..7284)
                     /locus_tag="MJF80_003480"
     CDS             complement(6793..7284)
                     /locus_tag="MJF80_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282092.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
BASE COUNT         2655 a         1127 c          743 g         3066 t
ORIGIN      
        1 attttgaaag cttttcaaga aaaacgtact ttttataaac ttaaagatgg aaactttata
       61 gatttatcag aaagagaaac aaaagatttt tttgaattag ttgaaaatct agatataatg
      121 gataaatcaa aaaataataa aattcataaa aataaagctc tatatataaa tgatgtaata
      181 cgtagtaaaa atttaaagtt tatagatggt aaaaaagaac tagacagtat atgtgaaaaa
      241 tttagaaatt ttgatagcat aaatttagaa attcccgcaa atttaaatgc aaaccttcgt
      301 gactatcaaa taaatggcct caattggttt aaagttttag attattataa atttggtggt
      361 atattagctg atgaaatggg tcttggtaaa acaatacaga ctattgcatt tttattatca
      421 ttatctaata aaaaaagtct tattgttaca ccgacatcac tcatttataa ttggaaaaat
      481 gaatttgaaa agtttgctcc agatataaaa gttttattga tacatggaaa taaaagagat
      541 agagaaaaat gttttatgga attagaaaat tttgatgtaa tacttacaac atatggtact
      601 cttagaaatg atttagaaaa atatagtgaa ataaagtttg attattgtat attagacgaa
      661 gctcaaaata taaaaaatcc agtagcttta gtaactgagt ctgtaaaaag tataaatgca
      721 gaaaataaat ttgcactcac tggtacacct atggaaaata atctactaga attatggtct
      781 atatttgatt ttataatgcc tggctatcta tatagtaaag caaagtttca ggaattgttt
      841 ataaataaag aagataatgt taaaaacctg aaaaaattga taaaaccttt tattcttaga
      901 agaagtaaaa aacaagttat gaaagaactt ccagataaga ttgaaaaaaa cttttttgtt
      961 gaattgaata aagagcagaa aaagatatat agtgtatatt caaaagatat acaagataaa
     1021 atgaaagata aaaatctaaa gaaagataaa atagttatat tttcttattt aactaaacta
     1081 agacaacttt gtttagaccc aagtatagta gtaaaagatt acaataaaaa aagttcaaaa
     1141 attgaaacat gtttagaaat acttagggat agtataaacg aaaaccacaa aatattattg
     1201 ttttcacaat ttacttctgt gcttaagaac ataagtaagg aacttgataa gtataaaatt
     1261 aaataccatt atatagatgg taaaactaat gccaaagaaa gacttgaact tgtagatgag
     1321 ttcaataata gtatggataa aaaagttttt ttaatatctt taaaagcagg cggaactggt
     1381 ttaaatctta catctgctga catggtaatt cactttgacc catggtggaa tccatcagta
     1441 gaaaatcaag ctagtgatag agctcataga tttggtcaaa aaaattcagt acaagttata
     1501 aaattgattg ctaaagggac tatagaagaa aaaataataa aattacaaga aagtaaaaaa
     1561 gagcttataa atcaatttat aaatggagaa ctttctaatg aaggtgtatt aaaaagtcta
     1621 tctgatgagg agataataaa cttatttaac taaattattt aatcctacag cttaactata
     1681 tgaatttgtt ctaacattta tatagttaag ctaaaatatc tgaataaata aaatttaata
     1741 ttgttattta atcccaactt tacaatctaa tgatatctct ttctatacat attgcatata
     1801 caaatgtaat aacttgacta ttagaatatt ttgttttaac aatcctattt catcataata
     1861 atgaagtata tgtacactca cttttgtaag ttttgcaatc cattttacag ttttcattat
     1921 tgtaaatttt ggttaaatta ttcctctaaa aatttctttg ctacatttga aaaatctaat
     1981 ccattcgata aaattgcagg atgaccacct ttatcaaaca aatacatttt accattttta
     2041 atcttttcaa gaagtaatga aaaagtattt tcaaaaaaat cactagatat tagagataca
     2101 aactcatctt ctctacttcc tgttaaaagc acctctggtt gcatagtttc tagaggctta
     2161 tgaaaaaact ttcctatagt tttataatgt tcaaaaatag cttctgtatc attatctaca
     2221 actttttccc agttttctcc ttgattataa atataaaaag cttttgcacc atcatcttgc
     2281 ttagatgctt cacgctctat tttcacattt tgtacaaatg actctagagg aacttctcct
     2341 tcaaaactat ctgcaattac cttattcact aaatcaggtc tctcaagtgc aacatttaat
     2401 gcaacaagag ctcctccact actaccaatt atgtttactt ttttatagtt cattgcttct
     2461 aaaaaacata tcacttgcat agcctcatca aaccataagt cagcagaaaa tttatcgacc
     2521 atttgagatt ttccatgacc tacaaaatca attaatatta ctttatattc atctttataa
     2581 aaattgatta tatcattaaa catcttggaa gaggctgtat ttccatgtaa aaaaagtaat
     2641 ggtgtaccat ccccaatttc attatagtag cactgtttgt cgttatagtt aaaatatgcc
     2701 atatatttcc cccctttaat atccctatag gtatatattt attacttata tttaaaatga
     2761 ctattccctt agccacttaa aaatagtata acttagaatg tagataaaat caacattcct
     2821 tgctaattta tctattgttt gtctaattta ttataattta acttctgtaa aatttctttt
     2881 ttgtacgaaa atattccttc catttacaac aatcattcaa atgaagtata acatgacgag
     2941 taggaggcat aagcaaaatt ccagcaatat ctttattccc ccaaaagtcc aacaaccaaa
     3001 tagaatcttc atgctttatt actcctccag tcaccaaaat cctttctaag tcaattggag
     3061 agacctttcg tttcatatct tcagctttca aatccttaac aacttctatt gtgttttttg
     3121 caacttcatt tcgataacaa agcaattcat caatattgac taaattatcg tatatctctt
     3181 ttcactttaa ccattttttt ctttataggt atctccttat atttttttaa tatcaaatct
     3241 cccttataat ttaatatatc tacatatgaa cataaatctt gaacttcaat tacacctata
     3301 tcatcattag ttataccttt tatattacta aatgcaccaa ctatatctaa aactcttatt
     3361 ttcttcttct taccagcatt taaatataat ttaacaactt cactttctat atttagtttt
     3421 atatctttac ttttgtaaga atttttatca actttttttc tctttgatac tttttttagg
     3481 attccttttg aatattcttc aaacaaaact ttgccatgtg taattctact tttttctata
     3541 tcttttaatt catttatttt atatcctata tacgtttcta tattctctat atatttttca
     3601 tccttttcag ttactatagt aattgccttt ccactatttc cttttctccc agttctacct
     3661 attctatgta tatagttttc tttatcttgt ggcacatcat aatttattac taaagaaata
     3721 tcatcaatat gtattcctct ggacgctaca tcactactaa ctaatatatt aaatttttgg
     3781 tttttaaaat ccttaattac aaaaattctt ctttcttgtg ataaatctgc atgtaactct
     3841 cttattaaaa aaccctcttt actcatcttg ttatataatt tagatacttt ctctttggta
     3901 ttacaaaata ttatcacact ctttggattt tcgctgtata ttaattcttt taaaaggatg
     3961 tacttctctc ttccatttgc cttgattatt ttgtcatcta tttgtcttgt tttttgatta
     4021 gtatcgctct cattttcttc tatatttata acactgtaat ctagcatata tttttcacat
     4081 atatatttta tctcttcatc tattgtagct gaaaaaagac ttactatttt ctccttaggt
     4141 aaatttaata atattttttc catttgttca acaaaacctt tattaaacat tttatctgcc
     4201 tcatctatta ctaaatactt tacattttcc aacttaatag aacctctatt tatatggtca
     4261 agtattctgc ctggagtagc tacaacaata tgaacccttt gtttcaattc agctatttgg
     4321 tcttttatag attgtttgcc aaatatagca ctacatctca ccttctttag tctccctata
     4381 tctgatatct catcttttac ttgcaaggct aattctcttg ttggaaccac tataagagct
     4441 tgaatattat tgtaatctac atttatattc tcgcataaag gtattgcaaa acttgctgtc
     4501 tttccacttc ctgtttttga cctaactact aagttttgac cttttaataa ttttggaata
     4561 acttctcttt gtactctaga aggtatatta tatcctaaac tcttcaatga cttaagtatc
     4621 ttttcattta acttaaattt ttcaaatgta tatttcatat attaccacct tattatattt
     4681 atcttatcta tatatttttc tacattatat tattataata caaggttcta ctatttttat
     4741 attatttatt agactgaaga taaaagtata tactctataa acctatagtt tagtagcaaa
     4801 ttaattaaat ttttatattt aaatacctcc ttaaatatac tgataaatca atattttcaa
     4861 ggaggtatct cttgtcaata atcaaactaa tctatctttt gaaagtttaa attatactaa
     4921 tacattgtat ttttatttat atctaatatt aaaaggtatt aatcaatacc taaatcacca
     4981 taatctcaca tatttatatc tttattcaat ataccttctg taatcaatcg tgctatataa
     5041 tcataacctg attcatttgc tttgttacaa tcattcacat tatcacagaa aaaactttca
     5101 atctgtattg caactggatt tgtatttctc aatatatata aattttctct ttttttagct
     5161 cctctatttt caaataattc tccaagtcta ttagatactc ttttagcata ttctaatcct
     5221 tcagtgccat agtaaagcac ttctgtacca cattgtgttc catcagcttt atttaaatgt
     5281 agctctacta ataaatcgta tttaccactg tttacttttg gaactctata gaaaaattct
     5341 tctctttctg acataaagtg cctttctgga caaattatta catctactgt attaccttga
     5401 cttataagca tctcagctaa cataggagct aattttttat tataattgta ttcatcaact
     5461 ataccattta cacctgtact tttaccacct tttagtatac tatgacctac tgttatgcat
     5521 attttcattt acttttttca ccttctttta agccaatttt tatataatag taatataatt
     5581 ttaaagttat tactaataat aatatattaa ttgaagaagg taaaagttct ccgtgattat
     5641 cattttttta acatatttgt cttattttat cataaatcta tattttttaa atattatact
     5701 atacttatta tttataattg tttaattaat catttatata tatgataaat atatcaatat
     5761 ctattttcct ttaaccaaat gctattttaa taagtttaag ttttagcttt aaaatactta
     5821 ataatacaat aaaagtgatg tatttacaaa taattcttct ggatttaatt catttattga
     5881 tgtatgttca atttttatat ttatattttt catatcttca taaataattc caactggaga
     5941 acaaatgaaa caagaaaatc caaaagtttt tgttattttt tttaccttat tatcaccaaa
     6001 acttatttgc tttttacaat cgtttatatt ttcattgtta ttttgtatat catttttata
     6061 atacttatct tttttaatgt cattagactt atttactgtt ttaaaattac tcctattttt
     6121 acaactatag tctatttcaa cttctatata cccaaaaact atcaagttac ataatttcaa
     6181 attactacct ttgtttataa gatttttcat aggtttaaca tcaaatattt tactatttac
     6241 atttactttt aatatatctc ttagattttt aggagctttt aaagtattac ttatattcaa
     6301 ttgtttgaat acaattttat ttacattagt ataaactcta ttactttcta ttgatattct
     6361 tgttgggaat ttatctagat tacacttata ataatagtat atattagcac tattctttat
     6421 aatcctatgt gcagaatttg gtaaaactac actcttaaaa gatattattt ttttacaact
     6481 tgcatctatt ctccctatgt aaaaaccttg ctgtgggtct aatccacgtt ttataatgcc
     6541 attaactttt accgtattat ttataaactc tatatattta gaaatataat ctttgaaaaa
     6601 tatatttcca gttttgtacc tacttgtatt tgtaacaata atagtgtagg tcaaaatctc
     6661 aaatacagat gcacattttt tatcaacata tttttctact tttataatac ttccatttgc
     6721 atcatttgaa aatacatcat taaaagtagt tttcgttatt ctcattgcat ccacctttct
     6781 ataatttaaa gattactcta tcttggaact attttcataa cttataattg cagtaacact
     6841 aataaataaa ttatcatcta aaatagatga agtaatatct tgtataagat actttaaatt
     6901 aattttatct ttattttcta tctttctagg catttgtata aatgtactaa agggtatttt
     6961 taatttaaat gtattttttt tactagaatt tctatttctt ttattgcctg cataatctaa
     7021 tattgcatca atatctatat caccaactaa aatcaacttc tttcctgtaa gttgttcatc
     7081 ttctaatgat atacattcta tagtatcaac aagacttata ttgttaatat tcatattgta
     7141 aacatatgtc tctaatgaat ttattacagt ttttttagaa gctaatttaa aatttttata
     7201 tgtattaaat tgagataaat aaagagtctt ttctagtttt tcacatcttt caaggttgta
     7261 tattacctta ttattttcat acataatcgc atcaatctcc ctgtatctat atattaatac
     7321 tattcataaa aattatttca aattaccatt ttttattatt cactatctag ttgttattta
     7381 cttttaagaa agaatccttt tttacttcat tgatttcaat ttacaagtaa aagtctttct
     7441 gaaaaaaaaa tatatatatc tctattcact tttttataag taattataat ttttatagac
     7501 taacggtcag ccttaaggga aaaggtgttc tttaagtgca gaatttagat aaagtaaata
     7561 atccttttat aaataattcc aagttttaac t
//
LOCUS       DAGSWI010000169         7569 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.172, whole genome shotgun sequence.
ACCESSION   DAGSWI010000169 DAGSWI010000000
VERSION     DAGSWI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.172"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(35..697)
                     /locus_tag="MJF80_003481"
                     /pseudo
     CDS             complement(35..697)
                     /locus_tag="MJF80_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(857..1393)
                     /locus_tag="MJF80_003482"
     CDS             complement(857..1393)
                     /locus_tag="MJF80_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBZ0282093.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSIRAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1466..1879)
                     /locus_tag="MJF80_003483"
     CDS             complement(1466..1879)
                     /locus_tag="MJF80_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBZ0282094.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2345..2899
                     /locus_tag="MJF80_003484"
     CDS             2345..2899
                     /locus_tag="MJF80_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBZ0282095.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISGIDDTISDMQGSISSMNGTISDMQDSITDMQVEIKEVNSRLDN
                     MEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVKDTSDIK
                     NDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3507..3686)
                     /locus_tag="MJF80_003485"
     CDS             complement(3507..3686)
                     /locus_tag="MJF80_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBZ0282096.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(3912..4445)
                     /locus_tag="MJF80_003486"
     CDS             complement(3912..4445)
                     /locus_tag="MJF80_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBZ0282097.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            4762..5112
                     /locus_tag="MJF80_003487"
                     /pseudo
     CDS             4762..5112
                     /locus_tag="MJF80_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6193..6765)
                     /locus_tag="MJF80_003488"
     CDS             complement(6193..6765)
                     /locus_tag="MJF80_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBZ0282098.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6827..7090)
                     /locus_tag="MJF80_003489"
     CDS             complement(6827..7090)
                     /locus_tag="MJF80_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBZ0282099.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(7090..7311)
                     /locus_tag="MJF80_003490"
     CDS             complement(7090..7311)
                     /locus_tag="MJF80_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBZ0282100.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2653 a         1286 c          884 g         2746 t
ORIGIN      
        1 gactaaagtc acaagtgtgc gtagctattt tttaatcaaa ctggaataca gactccattc
       61 ctaggaaatt ggctgaaata tagtctgctg tcaaagatgc tgtcatcaca gaaattaaaa
      121 cagatactga aaagttttta tggacttctt caagtgaaaa cataacaccc gccagtggtg
      181 catgaaaggc tgctgataat ccagcacttg ccccacaggt taacaggaat ttttcctctg
      241 tcttccctcg gtcaagagct ctagaaatac ctttacctgc catagcacca agctgaatgg
      301 aaggaccttc ccttcccaaa gcaagaccac ccatgagtga taagaatcct ccaataaatt
      361 ttgttggcaa cactcgccac catacttgtt ttattttccc tgtcatttcc ccctcaagct
      421 gagggatacc acttccagaa atcattggct cccagtccac caactttgat acaatccatg
      481 caagtactgc aagtactgca aaccaagcta ttatttttat tgtagaacct tctacaaatt
      541 ttaaaatttg gctaagccaa gtaccagcct gccctaaaag tatcctgtat aaaagcacaa
      601 tcaaaccagc cacagcccca accataagtc cttcaccaat caaaattacc tgaaagtgct
      661 ctgcccgttt gataatatga gatgtatcct ttttcatact attcctcctt catccaataa
      721 ttctacacaa tctgaatctc caatgactaa agtcatgagt gtgtacagat attttttaat
      781 caacaaaaat agatttttac ttactaaata taagaaaata attgctcaat ttctcaataa
      841 atgtgaattt attcttttag gtatttattc tccacagtcg tgcttcttcc agcaaaatat
      901 ttattaattc ttgctttctc tgtgtataga ttttacgatt gtcctcaaac tgtaatgcta
      961 atttttgttt atgactatca tagagttttg ctttgtctgt aaaagtattt agataatctc
     1021 gaaaattgat ataattattc catgcaacac tattccattc aacaacatga atatggtgag
     1081 tacgagtatt tttctcaaaa tctcccatag caaaaagtaa ttgttttggt acatcctgac
     1141 ctcgaaaaac gattccacat tgctctaacc tttcgaggta taatttagct tcctctaaag
     1201 aagcaacacc tacaacaata tcaataatag gctttgccct aatactggga atagaagtgc
     1261 ttccaatgtg ttgaatatca atggcagtct tatcccatat acttttcaat aatataatga
     1321 catcttttgc acttttatcc catatcatct gatgttccaa taatttaacc ataccacatt
     1381 ttaaacctat cactatatac cacctcaata taatccacct ttatatatat tacaattttt
     1441 tctatcttga taaacagtga tttagctatt tcactttttc ttgacactcg ctacattccc
     1501 tatcatgaat agatataatt cctccacatt tagaacaagt atatctctcc ttctgctttt
     1561 gcataaactt ctctaaaccg tactgctgaa caaatttact attttccata aggcttgctt
     1621 gatatctctt gttataactt ttttcaagat tttttatgag tttacaagga aaattgaaac
     1681 attcaaaaca ataaaacaac cctttttgac agacacaatc ctttatttta cacttacggc
     1741 agtgttctgg ctttcctgta tcgccattca aacaaccagc acatggcttt ttgtgataac
     1801 aatgcttata gcaaaccata caattcattc cacaaggagc aaacataatc gtatcaatac
     1861 tctcttttgg cattttcata acattttact cctttcaaaa atccaatttg tcgaattgtt
     1921 atatattcat attgtaacat aaaaaatagc taaaaaattc atgcactttc attgccaata
     1981 aattttcaag gaaatatgaa agtttaaaac taagaataaa aagagataaa aaggaagcta
     2041 ctaatcaaaa tattcaaata caagtaatca atataaataa cttaaggtat atttaaaagt
     2101 atatgatatt accagcagtt ttaaatacaa atataaccac acatgcctca aaaacactta
     2161 ttacagtatc ttagaaactt agacaataca taagattcat taataaaaaa gttctcaata
     2221 tagatatatt taaccatgtt ttgaatagca gataattata tgattaaaaa ttaaatatga
     2281 catagcgttt gtggaatata acacctaaat tatgttataa taataaaaca ggagtgtgat
     2341 agtgatggat gaaaaaatat tagagttatt acaggaaatg caaggtaata ttagtaatat
     2401 gcaaggtagt atcagtggta tcgaaggcac tattagtgat atgcaaagca atattaatgg
     2461 tatcgaagac actattagtg gcatgcaagg cactattagc gatatgcaag gcagtatcag
     2521 tggtatagat gacactatta gcgatatgca aggcagtatc agtagtatga atggcactat
     2581 tagcgatatg caagacagta ttactgatat gcaagttgaa attaaagaag tcaatagtag
     2641 gttagacaat atggaacctg atattaaaga cttaaaaact ggacaagttg aaactagtag
     2701 tagacttgat actattcaga gtgatgttac agaaattaag tcagatgtaa aaattaacag
     2761 aaatacatta gatacagtga ttaaacatac ctcttcatta gtaaaagata catcagatat
     2821 taaaaatgat ttaaataata tagagatagt cacatctaag aattcgaatg atattgctaa
     2881 attaaaatca gttaaataat ataccatagt aaaaaatacc tgaaaatttc acatacttct
     2941 ttaaagttat accaaatttt atacagaaat actgattatc cgacaacaca ctttacttat
     3001 aaataattta ttaaaaaaat actcatttta tcctaaaatt aacacgacta aatgagtctt
     3061 gatataataa taaaaaataa gggagattga acactaaaaa gttgtgtatc tcccttatat
     3121 tctataatat agttatataa tttttcagcc taaaataaaa gtccatcttt gattaaaaca
     3181 catcccctta tcacttataa aaggataaaa tcttgttttc atttttccat ttatatcttc
     3241 ttgcttaaga gtaataattt tttcttttga tactttaagt gtttggttaa atttaaccaa
     3301 tcgactaagt tttgttcaac atctattttt catattttcc aatacttaat ctatttttta
     3361 taaataattc tttcatttct aactcactta cattttttac ataaaacgaa gcatataaat
     3421 ttcaacctta tcttgtatgg ttcttaatac ttaatgagta gcttaagtat caaacattat
     3481 aaattttccc cttacattta acactcttac catttccaaa atatacaaaa tatattttga
     3541 acatttgtac aatgatgaaa tacaatacat gaaaatacaa tatcataact gttgtctaaa
     3601 aatagaagtt cttcaacata acctttcaca aaaaacatat tatctaaata ttttttgtct
     3661 gctccttgtt ttcctgtttg tagcatataa attactgcat ctaaacatat aactactcac
     3721 atgaaaaaag aaaataaact tccaaaatac aagctctaaa aactacctct aaagttgtat
     3781 aatgtttact aggaataagg ttgtataaca tatagtttaa ataatcattt tttaaaattg
     3841 caagttattg ctttcaaaat tagatgtttg tatatcaaat aaatgattaa gaatttctaa
     3901 atttaatggc tttataatgc gctccctttc tttgggataa aataatgttg cacagatact
     3961 ttttctaaag ataacaattt tatttttcta tcaattccac cagcataccc agtcaaactc
     4021 ccatttgttc ctacaacacg atgacatgga ataataatag agattccatt atgccctact
     4081 gcaccaccga ttgcttgtgc tgacatttta gatattcctt tttgttgagc aactttatat
     4141 gcaatttctc cataagtgat tgttttacca tatggtattt gttgtaaaat tttccacact
     4201 gtagtctgaa aaggagtacc taccatatgg agtgatggtg taaaattggg ttcttttccc
     4261 gaaaaataaa tatctaacca tttctttgtc tgctcgaaaa ttggcaattc tttttcttga
     4321 tgttcttggt caagattata agcataatac ttttgattct caaaccaaag accagttaac
     4381 ccaatattat ctgatgctaa aagaatattt ccaattggac tatggtaata gctaatatat
     4441 tgcatacaat tcctccttat attctgaaaa cttcactcaa atcattagac acttcttttc
     4501 tatctaccat tatgtaaaaa catactaata taatttcctt ctaactacac tttcgttata
     4561 atatctatcc tcatatcata ttagttttat atttacctgc atctgttcat ccttttttcc
     4621 ctttcccttt gatgaatata aaattcatac atttaagctt cctactgtat taacttttat
     4681 agaagtgtat attataattt tatcactaaa taggccaaaa aaatatcgat atagcatcaa
     4741 gtaatatcat aatactttca aatgggaaaa gtaagaatag gctgtaaaaa agaaaagtat
     4801 ttttatgtta gaaatccagt aaaatcatta gtagatagaa taacagaaaa cgaacaattt
     4861 gctatttatc attttacaca taaatataat tgacgcaata aagtgttttc tattctacaa
     4921 cgattaataa ggttagaaaa aagtaaaaat gaactgtata tgtatgaagc attagtttct
     4981 gtttctttcc tttggcttat tatgataaaa aatattgttt tgctaccaat aaaacacaaa
     5041 aatataatta atatccatat gcaaaaaatt ttacgttata ttgaaaaata ctattgtttt
     5101 acattatatt gaagggtact attgtgaaaa cttaattttg gagtactatc aagcagtggt
     5161 aatatcaata aatcagagtg ctccagatga tttgaactaa gtttaaatac tataccttat
     5221 aaatatttta ctgagttttg tttattaaaa gcaactcaat ttctaaaagg cacaaatgag
     5281 ctaataagtg acattgctat aaaagttgtt tttcatcaaa taatctattt tagtaaatgt
     5341 tttaaagaaa aaacgggata ttctccaaaa gtgtacagaa atataaaaaa agagatatga
     5401 ttttttattt cttttgatta tccaaagagt aaataaaaaa tattgtaaaa aacttactaa
     5461 caacggcaat gctttgaact atggtaatac tttgaacttt tgattggact ttttttgcta
     5521 aaataatata tttaaatagc aaagtaatct gtttaatcaa taaaaaataa gagataaaag
     5581 aaatcaacta aaacttttac ctcttatttt gatagtttat cagagctaat atttacactc
     5641 tttttatttt tgatacttca ttaaaaatga tgcttactct ccattcactt ttaattcttc
     5701 attaaaaatg atgcttattc tccattcact tttaattctt cattaaaaat gatgcttact
     5761 ctccattcgc ttttaattct tcattaaaaa tgatgcttat tctccattca cttttaattc
     5821 ttcattaaaa atgatgctta ctctccattc gcttttaatt cttcattaga actatgctta
     5881 ctctccattc acttttaatt cttcattaaa aatgatgctt actctctgtt cacttttaat
     5941 tcttcattaa aaatgatgct tattctccat tcacttttaa ttcttcatta aaaatgatgc
     6001 ttattctcca ttcacttttg attcttcatt aaaaatgatg cttattctcc attcactttt
     6061 aattcttcat taaaaatgat gcttattctc cattcacttt tgattcttca ttaaaaatga
     6121 tgcttattct ctgttcactt ttaattcttt attaaaactg atgcttattc tccattcact
     6181 ttcaattctt cattaaaact gatgcttgca ctctgtaaac tcataaacat ccattaatgc
     6241 ttcaccaaat ctttgatagt gaactacttc cctctcaccc aaaaatctca atatatcttt
     6301 caaatcaggg tcatcagtta aatttattaa ttgatagtac gtagccaatg ctttttgttc
     6361 agcagccata tcctcatgta aatcagttac cgaatcgcca aagacattaa tataacttgt
     6421 agtaaagttt actccattag aatctactgg ctgaagacca tgtccaaaat tagcataatt
     6481 actaccaagt ccagcagcct taagttcttc tggagtacaa ttgcctatta attgatataa
     6541 cactgaagaa attatctcaa catgagccat ttcctctgta cctatatcag ttaaaagtgc
     6601 acgagatttt ccagttggca tagtatatct ttgttgaaga tatctagttg cagctgccaa
     6661 ctcaccatta ggtcctccca actgagtcat gatatatttt gccattctag ggtctgcttg
     6721 tcttatgtta actggatgtt ccagtgtttt ttgataaatc cacatattat cttactcctc
     6781 cttaatatat atttaataat aaatctaatt ttaaagttta ataattttat ttataaaatg
     6841 atttgtccca tggccaaggt tcagatatcc atgaccaagg atattcactt gattcatatc
     6901 caaaattagt taatgctcca tatttagatt cataatcaca cacagcttgt gtaaatttat
     6961 cgcagtatct gttgtatgaa tcaagagctt tcttatccct tggattgtta tctaaatata
     7021 aatttaattc tatacaggca aattgatatt ctgaaattct ctctaaaagt tctcttctat
     7081 ttaatgacat tagtacatgt tacccccttt tttagctcta actctatttt taggatttgc
     7141 atatagccaa tcattatagt tatttgactc tattaaatct aattcaggaa ataaagtacc
     7201 tctttttaat gctgtttttg gattgtatgt gtcatcataa atttgacgat tgatgtatgg
     7261 tctagcaaat tcacaacaat agtcttgttt atcctctttt aaatatccca ttaattatcc
     7321 tccttaattt ttagaatttt aaaatagcat tatttttaaa aattgtatac tggttctata
     7381 ttataactgg tagttttaat aggtctttaa taagattaaa tgattaaata agtatctttt
     7441 tttcttattt tacctttaaa atttttctaa ttaatctttc ataaatatct attttaaata
     7501 aataatatag agtttataca attttttata gcttaattac tatttactat atattattca
     7561 tttttaaat
//
LOCUS       DAGSWI010000170         7483 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.173, whole genome shotgun sequence.
ACCESSION   DAGSWI010000170 DAGSWI010000000
VERSION     DAGSWI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.173"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(476..865)
                     /locus_tag="MJF80_003491"
     CDS             complement(476..865)
                     /locus_tag="MJF80_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0282101.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(2625..4031)
                     /locus_tag="MJF80_003492"
     CDS             complement(2625..4031)
                     /locus_tag="MJF80_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0282102.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLVLKDGKFILDDSSKEILHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(4076..4783)
                     /locus_tag="MJF80_003493"
     CDS             complement(4076..4783)
                     /locus_tag="MJF80_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282103.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     KVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKEGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            4954..5868
                     /locus_tag="MJF80_003494"
     CDS             4954..5868
                     /locus_tag="MJF80_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282104.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            5868..6629
                     /locus_tag="MJF80_003495"
     CDS             5868..6629
                     /locus_tag="MJF80_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282105.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            6641..7378
                     /locus_tag="MJF80_003496"
     CDS             6641..7378
                     /locus_tag="MJF80_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282106.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         2548 a         1208 c          806 g         2921 t
ORIGIN      
        1 acttttgttt ctacctcttc agtatcttat atcaatcttt tattttttca atatcttctg
       61 ttaacacttt tatctctatc tctttaatat cttatattaa tcctttattt ttattttttc
      121 aatatcatca ttaattccta tttattttat catcttaaat acgaaaacac tgtgtcatat
      181 ttgcaaaata tatattatca tctatattaa agcttcacaa tttatattta aaaattctct
      241 ttaattacac tacctctatt taaaaagcta ccttaattat acttataagt aaaggtagct
      301 ttttaaattt aattaaatag aatatccact attaatttat atttaattta tttagattca
      361 cattatagta tcataatcca cttataatca tttgtcatat tacttaagta gactaatata
      421 ttacataaat acacttacat acactttata ttgatgctat aagaaatacg tttgcttata
      481 cgtctttctc ctcttctttt agatttttta tccactcttc aataagtaaa atatcatctt
      541 tgctcaaatt aatattttca tgtaatgaca acaataaact ttttatagaa gaatcatgaa
      601 tatttctaaa aaaatctctt gtctcaactc ctatgtattc cttttcttta attaaaactt
      661 cataatgcgt atatttatct atcttttgag aatttagaaa tctttttact actaatctag
      721 ataaaagagt caaagttgtc gtttgtttcc attgatattt ttgctccatt cctaaaacaa
      781 tatctctaga tgttactttg gaatctgatt tccatataaa tttcattatc tttaattctg
      841 attgtggcaa cttttttata gtcatgataa aaccttgcta ctcagaatat agacaagcaa
      901 tatccataat aaaatttttc gattaaatac atcttatacg gatttatata agctcagact
      961 tataagatat ttttcaatct taatcctaaa tttagctatt tgtacacggt aaatatatat
     1021 aaataatgtc aaaattctct tgtattatat tgcttaattt tatgaaatat tttgtactca
     1081 gctatctatt ttggatactt gtccagtagc tctcctttct tttaaactct ttgattttaa
     1141 atatttgtat ttcagtaaat cctacttcct aaatttttta tttactacaa cttattactg
     1201 aacttcctac ttcctaaact ttttatttac tacaacttat tactgaactt ctcacttact
     1261 aaatttctta cttactataa attataacta aacttctcac ttactaaatt tcttacttac
     1321 tataaattac tactaaactt cctactttct aaatttctta cttgctacaa attattacta
     1381 aacttcctac tccctaaatt tcttacttgc tataaattat tactaaactt cctactttct
     1441 aaatttctta cttactataa attattacta aacttcctac tccctaattt tcttacttac
     1501 tataaattat tactaaattt cctactttct aaatttctta cttactacaa attattacta
     1561 aacttcctac tccctaaatt tcttacttgc tataaattat tactaaactt cctactccct
     1621 aagtttctta cttgctacaa attattacta aacttcctac ttcctaaatt tcttacttgc
     1681 tataaattat tactactaaa cttcccactt actaaatttc ttacttacta ctacttataa
     1741 ctaaacttcc cacttcctaa atttcttact tactaccact tatcactaaa cttcccactt
     1801 actaaatttc ttacttacta ctacttataa ctaaacttcc cacttcctaa atttcttact
     1861 tactaccact tatcactaaa cttcctactt tctaaatttc ttgcttctta agaataatgt
     1921 taattttaat tttaagatta tagtttttac cttgtttaat tgaaacattc tatataatta
     1981 tatccttcta ttttagagtt tttttattta tatattacaa agtttaaatt ttttcattaa
     2041 ttatattata aattttccat atatttcaac ataaatacat atatatatat gttatttcta
     2101 aaaatactac acaaaaactc tactttagta gaacttaatg gcgcaaaata cttcaattta
     2161 aaacatttat cgtactttaa ataaataact ataataatat acttacaata gttacataga
     2221 agctattata actacacaga acagcgtata attaaaactt ttttaggatg tcattatatt
     2281 ttttcttaaa attaaaaaac actataccat aattctattt tagtagaatt tttttgtcaa
     2341 taataaaatt aaatattaac taaaaaataa aaaaaatgga attatctaaa gaatttaata
     2401 accttacaat aattccaaaa tattatttct atgaaaattt tgataccatg agtaactcag
     2461 ccataagcgc ataaagcaca agttactctt tactttaata aaacttgaca agaaaagaat
     2521 ttatccttat tctactctaa tagagttaat ttgtcaataa agaataaaat tattttcaaa
     2581 atattttcat tcttcattct tatctgttat tattttataa aatatcactt aattagaggt
     2641 aattttagta ttgtttttag tccctgacta tcaatgctcg taatatctaa tttacctcta
     2701 tgtgcattaa taatgccaga tactatcaaa atacctagac catgattttg acctttggca
     2761 tatgcctcac ttagtccatt ttggctcttt aataaactat taattctttc ttgactcata
     2821 ccttttccat catcttcaac aattatatag caaaaatcta aatcagactt agaggtaacc
     2881 cttatattac atccttgttg gttatgagtt atactatttt gtattaaatt actaatagcc
     2941 ctttttaata attttttatc tgcatctata ataatatctt catttagtat atcaagttca
     3001 acgtaaaagt cattatccaa attattattt ataaactcag aaataagctc tctcaaaaac
     3061 gcagaaactc taatcttatc acaattcaat gcttgcatat tatattcaag tttagacact
     3121 aaatttaggt catttactaa ttctctgatt ttaatccctt gattgcatat aatagacact
     3181 tgctcttgtt ctctatttga aaggctactg ctttcttcaa gttctccagc atatccaatt
     3241 atcattgaaa gtggagttct tatatcatga gatattcctg caatccagtt agacctagct
     3301 tcctctttct tcctaagttt ttcatctttc atttgcaact ctttagatac agtattaata
     3361 gattttgaaa tatcacttag tattcctttt tcttgtaaaa gtactggctc tccttttgac
     3421 ataagcttta tgccagtaat aattggtttt atagatttca ccatttttaa acctatgaat
     3481 agagctatta aaaaagtaac tattgcatta accaaaaaca tacctattat taacatagga
     3541 aattgttcta tttccttaac cgtataagta aggttaaact tagtgtactt gtcttttgga
     3601 taacctgtta ctaatattcc atttggatgt tcccatacat acacaggata gtcctttaaa
     3661 aagaaccttg aaaactttgc tacatctgtc aaagtataat gacttggaat ttcttttggt
     3721 aaatcaaatc cccatattct atctccattg ttatcaatta acattgccca aactttattt
     3781 ttatgtagta tttccttact agaatcatct aaaataaact ttccatcttt taaaaccaaa
     3841 ttatcttcaa tcataatcaa gtttgatttt atttctctgc tatcacttgt atctaaatta
     3901 tcagagtatt tagatacaaa taacatatat gaaaacagaa gaaaacctgc tatatttaat
     3961 acaataaaaa gtaatgaaat caagatactt gaacctataa atcgcctcaa tatgtacttt
     4021 acactatcca aaattaaatc actcctcttg atttctttta ttctaatatc taaaattaat
     4081 taatcatcaa cttatatcca agacctctta ctgtaataag atgtttaggt gtagatggag
     4141 ttttttctat cttctctcta acccttctaa catgaaccat aagggtattt tcatatccat
     4201 acatatcatc tccccaaatg gcttgacata gtgaatcatt agtaacaatt ctccctttat
     4261 tttcatacaa ctttaaaagc aacgaatgct ctttagctgt taaagaaatc tcttgccctt
     4321 ctttctcaac acatgcacta tttaaattca caacaacaga atctcccagt ttaaatactg
     4381 gcaactcctc ttcttctata ggcacatata cacgatttaa tatacccaca agccttaaag
     4441 taagttcctt tggtaaaaat ggctttacta tataatcgtc agcccctaag cccaaaccaa
     4501 tcaatctatc ttcatcttca cctcttgcag agagaaaaat cactggtatt ttagatgtct
     4561 ctctaatttt tctcataata gaaaaaccat ctccatctgg caatacaaca tctaaaattg
     4621 cacaatctgg ctttacattt ctaaatacct taacagcttc ctcataactt gatgctaaaa
     4681 atactctact aaatccctct tttctcagga tttcatctat catttttaat aattccttgt
     4741 ggtcatcaac cacaagtatt tttttatctt ttattgactc cataatcatc accctccttt
     4801 agtataccat atacccattt tcacaacttt gcctaaatag acttttaagg ttattgtaag
     4861 gtaaatttca gctaattgaa atgtaaccaa gttattatac tgatataagt taaaagaatc
     4921 aataagcaca gattaaaaaa ggggatgaaa catatggaaa tgataattag aacacaaaga
     4981 ctttcaaagg aatacaataa aatatttaga gtaaaggata tagacctaag agttccacaa
     5041 ggagctgtat atggtttttt gggtcctaat ggagctggaa aatctaccac tcttaaaatg
     5101 cttcttggac ttgctaaacc cacacaagga aatattaata ttttaggtaa agagttaaat
     5161 gaaaaaaata gaatatcaat tttaaaagat ataggttctt tgatagaatc tccatcatac
     5221 tatggtcatc ttacagggct tgaaaacatg accataatgc aaagactctt agatttacct
     5281 aaaaaaaatg taaatgaagc attaaaaatt gtaaggttag aaaatcaaaa aaataaaaaa
     5341 gttagtcaat attctttagg tatgaagcaa cgtcttggta ttgctatggc aataatgaag
     5401 tttcccaaat tattaatact tgatgaacct acaaatggac tcgacccagc agggattgag
     5461 gaaattagag aattaataaa atctctacca gaaaaatacg ggatgactgt tctaatctct
     5521 agccatcttt taagtgaaat tgaccaaatt gccacatcaa taggaataat aaatcatggc
     5581 gaactggttt ttcaagatag cataaaagaa cttcataata gaggtcaaag tcagattgct
     5641 atcaaaacta agaatgttgt atctgcacaa aaactattaa atactaaagg atatatgtca
     5701 cttatacaag atgggtactt gactattgaa aatttaaaag actcagatgt ggctaaaata
     5761 aatggtatgc ttgtccattc aaatatagat gtatttagaa tagaagaaca taaaaaaagc
     5821 ttggaagata tattcttaga acttactggt aaggcggtga ctttataatg aaataccttg
     5881 gtctagaatt ttataaactt aaaagaaaga agattattct gatgaccttt ttatttgcag
     5941 ctgttgaaat aatgtggtgt atcgtaaatt caaatagagc tattacacgt aatccagata
     6001 tgttaggtgg atttgaatat tcctatatgc ttatgagttt tgcaagttta aatggtctat
     6061 tctttcctat cttaatatca ataatcacat cacgtataag cgatatagaa cataaaggag
     6121 atacatggaa gcttttaaaa tcttctgtga catctttaaa tagtatttat ttatctaaat
     6181 ttttatgttc tgctattttg gtatctatac ctgtattgat gcaagtgtta tctattgtgt
     6241 tgtttggaca ttttagaggg gttattgagt caatttctgt atctttactt ttaaaataca
     6301 cattaggaac aattcttgta agtattgcta tcattgcact acacctatgg attgcaactg
     6361 taattccaaa tcaaatgatt gcaattacat ttggcatgat tggtagtttt ataggtttga
     6421 catcaggttt gttttatcaa ggtattcgta gattgtttat atggtcttat tacctagagt
     6481 tatcaccttt aagttatgct tatgataatg ttttaggttc atctgtctat agaattgata
     6541 tgaatttttc tatcacgatt ttagttttct taattggaat gctaatttat tatattggaa
     6601 gaaatcattt attaaaaaaa gaagtatagg agggatttaa atgttaagga agtcagtctt
     6661 gggagaaatt ctaaagttaa aaaattcttt tatatggtat gtattattag ctttgccact
     6721 tataagtgtg ttgattggtt ctggtaattt ttatctaaat caaggcatac tcaaaaaaga
     6781 atggtacagc ttatggactc aagttagtct tttttatggg gaatttttct ttcctatctt
     6841 aattgcaatc ttttgtgcat atatttgtag gcttgaacat atgaatcata attggaataa
     6901 tgtaatgaca ctgccagtta atttaaaaaa tatcttttta tctaaactta cagtagtttc
     6961 aatacttaca gctattacac aagttttctt tgttatcttt tatatagttg caggaaatat
     7021 gtttggtttt tctggtagtg tacctaaaga attgactggt tggattgcta aaggttggat
     7081 tatttctatt tcaatagctt ctatacaatt atacatgtct ataaagatta gaagttttgc
     7141 aactcctatt ggtattagtc tttgcctttc tctatttggt atgggattaa tagtagcaaa
     7201 acctgctgtt ggtatatttt tcccttattc tatgttaggt agtgctatgg gggttataaa
     7261 gcaatctgct ttaaatgtta atgacactat agttacaata tgtgttgcat ttattactac
     7321 tatagtattt tctgaaattg ccaatagaaa ttttagaaag aatgatgtta tttcttaatg
     7381 gtaaatgcta gatatttttg ggtttgaaat ttatattctt tttagttgaa atctatatcc
     7441 attttattta aaaatatata tcttttattt gaattttata tgt
//
LOCUS       DAGSWI010000171         7424 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.174, whole genome shotgun sequence.
ACCESSION   DAGSWI010000171 DAGSWI010000000
VERSION     DAGSWI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.174"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            49..954
                     /locus_tag="MJF80_003497"
     CDS             49..954
                     /locus_tag="MJF80_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBZ0282107.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKNVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            1078..2031
                     /gene="msrA"
                     /locus_tag="MJF80_003498"
     CDS             1078..2031
                     /gene="msrA"
                     /locus_tag="MJF80_003498"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBZ0282108.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNFHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            2184..3314
                     /locus_tag="MJF80_003499"
     CDS             2184..3314
                     /locus_tag="MJF80_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0282109.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(3431..4399)
                     /locus_tag="MJF80_003500"
     CDS             complement(3431..4399)
                     /locus_tag="MJF80_003500"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBZ0282110.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            4734..4922
                     /locus_tag="MJF80_003501"
     CDS             4734..4922
                     /locus_tag="MJF80_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282111.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTCFKNII
                     EVMYISKSLIMDEISKRD"
     gene            5182..6381
                     /locus_tag="MJF80_003502"
     CDS             5182..6381
                     /locus_tag="MJF80_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBZ0282112.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            6433..6936
                     /locus_tag="MJF80_003503"
     CDS             6433..6936
                     /locus_tag="MJF80_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0282113.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            7043..7360
                     /locus_tag="MJF80_003504"
     CDS             7043..7360
                     /locus_tag="MJF80_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282114.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
BASE COUNT         2946 a          721 c         1276 g         2481 t
ORIGIN      
        1 ttgattaaaa tttaaaatat aaggtgtttt atgtgggggt atatgtatat gaaaaattct
       61 aattttaata tattggcatt tgatggtggt ggattaaaag gggcattgag tatatccata
      121 cttgagagga tagtaaaaga gtatccaaat ctattaaaca atataaatat gtttggaggt
      181 acatctacag ggagtctaat tgcactaggg cttgcgtatg gtgtaagccc taaagagata
      241 aaagaacttt actcaattga aaattcaaag tatattttta ataaaagtta tgcagaaata
      301 ctcagaccaa agtatgaaaa caaaaactta aaagaagttt tattaagtat atttccagag
      361 gaactagaat taaaagattt aaataaatta gtaatgatac caagttttta tataggaaat
      421 gaggaaaatg catggaaacc agtgttttat aacaatatgc caaattcttt tacaaagact
      481 agcaaagttg tagatgtagc aatggcaagt agtgctgctc cggtattttt tccaacatat
      541 aatcgtcatg ttgatggagg aataatagca actgacccga gtttagcgtg tataattcat
      601 gccattgaca gtggatttaa acttaaaaat acaagacttt tttcaatagg gactggatat
      661 gtatataaca gtataaaggc agatactact gaatggggag ctattgactg gataattaat
      721 aaagaacccg atttaccaat tatatctatt accttagaag gaaattctca aatgagtcag
      781 ttattttctc aaaaacttct tggagataat tactatagat taaatcctaa gatggaaaaa
      841 aatgtagcta tggatgattg tgatgcatta gaatatctaa tgtctttagg agaaaattgt
      901 gatataaaag atgcttttgg atggataaag aaaaagagct tttatagaaa ttagattcaa
      961 tttgcaattg tatttttaca catgatttca ttgagtaggt aaaaataata aaatgcagat
     1021 tattaagtta cttcttaggg aataaataaa gtataattta tttaaggagt gatgaaaatg
     1081 ataaaacttg ctacttttgc aggtggatgt ttttggtgta tggtaaaacc ttttgataag
     1141 tatgatggag ttattaaagt agtttctgga tatacaggtg gtcatgttga aaaccctaca
     1201 tatcaagagg tatgttcagg aactactggt catttagaag caattcaaat aacatatgat
     1261 gatgatataa taagttatga tgaacttcta aatatcttct ggaaacagat taatcctaca
     1321 gatgatggtg gacaatttgg tgatagggga actcaatata agactgctat attttatcat
     1381 gatgaagaac aaaaaaatat agctttagag tcaagagaac atctggaaaa tagtaaaata
     1441 tttgatagta aaatagtgac tgatataatt ccagcaacta aattctacga agcagaagaa
     1501 aatcatcagg attattataa gaaaagacca ttaaactaca atatgtatta taaagggtcc
     1561 ggaagatata attttgtaaa aagtcattgg gatggaaaca atataaatag ggaagagtta
     1621 aaaaagactt taactcctat acaatttgaa gttactcaaa atgatatgac agaagtacct
     1681 tttgaaaatg aatattatga caataacaaa gagggaattt atgttgatat tgtaagtgga
     1741 gaagtgttat tttcttcaaa agataaattt gattctggat gtgggtggcc aagtttttca
     1801 aagccagtta tagataaatc tatcattgag agaagtgatt tctcacaagg tatgtacaga
     1861 gttgaagtaa gaagtgctaa aggaaatttt catttaggtc atgtatttga tgatggtcca
     1921 aaagaacttg gaggtttaag atattgtata aattctgctt ctcttaaatt tataccaaga
     1981 gaaaatatgg aagctttagg atatggagaa tatttaaaac tttttgagta attaggaata
     2041 tttattataa acttatctaa aaggtgaaaa tctaaaaaaa tattttcacc ttttgttttt
     2101 ttgtcaatag tatgataaat tattaattgt aatcttattt ataccaatag attaagaatt
     2161 taattttgaa agagagaaaa caaatgaatt taaaagactt taatggaaag agattaaaat
     2221 cagctagaat ttttagagca aagacgatag aacagttgtc taaagaaact aaaataaata
     2281 aaaaagattt aaaagctttt gaggaaaata agtatgtacc aaatatagaa aatacattaa
     2341 aattatctaa tatattaaat tttccaaaag agtattttta taaaaatgaa aatataaata
     2401 tcgttgtaga ggatagccac tttaaccctc aatctaaact acctagagta gaagagattt
     2461 cttatagaga aaaaataatt ataatacata aaatatattc atttatggag aattatatta
     2521 agtttccaaa agaaaacttg ccaaatagaa aagtaatgtc agatattaat ataaatgata
     2581 tagaatctct tgcatataag actagagagt tttgggattt aaatagtact ccaatagtaa
     2641 atatggtaag tttgttagag tctaaaggga ttataatatc aggtatgaat gtagatagaa
     2701 aaggggcaac tatctttact caaaaacaga gaattagtaa agaatctaaa tatctaatat
     2761 ctctgggtaa tgacaaaaag tcagcatcaa ttagaaatta tactttggca tgtgagcttg
     2821 gatatataat atcaagtgaa ttaagaatac caagtaagca attttcagaa gatgagtatg
     2881 cctgtgcatt tttattgcca aaagagagtt ttttaaaaga tttgactcat ccagaagatt
     2941 tagattatta tgtagaacta aagaaaaaat ggattgtacc tatatctgct atgattttta
     3001 gagcatataa tctagaaaag ataaattaca aaaaatacaa ttatcttatg aatgaaatgg
     3061 ataaaaaagg ctggttgata gaagaaccac tagacaaaat gaaaggttca agcccaacgt
     3121 atttaaaaag agcagttgaa ttattgatag aaaataaaat aatgagtgta aatagtatag
     3181 tatcaagttt agaggaattt ggaataaatc tttatccaga agacttagaa ttattgatgg
     3241 gattgaaaaa aggattgtta tctcaagagg taaataaaaa aagtaaagtt ataaaatttg
     3301 atggtaaaaa ataggtttat attttagtat taaaattaaa agcaggtaca aatgatttaa
     3361 acattaactt agttaaaagt tttttcaata agtcatttac ctgcttttag tgtaagaaag
     3421 aatattgaat ctaaagtgtt tttgaaagtg caatatattc ccttaaaaca gaacaagatt
     3481 tattaaattc gttttgctca tattcagtca tattgatttc aataatttct tcaataccat
     3541 cttttcctat aacacatgga acacctgcaa atacatttct ttcgttatat tgaccttcta
     3601 gtaatgtaga aactggaaga acttttttct cattgtgtag tatagctttt acaatttcta
     3661 ctaaagcagt acctatacca aattctgtac aatctttacc atttattata cttattcctg
     3721 tttttttatt tttttcaact attgatggta agtctaaatt gctataagta cttgggttgt
     3781 cttttatcat gtctaagatt ggtttaccac caattgaaac atgagaccaa ggaaccattt
     3841 gagaatctcc atgttcaccc atagaataag cttgaattga ttgttgagcg attccagttt
     3901 cttcagataa aatccttcta agtcttgttg aatctaaagc tgttccagtt ccaatgactt
     3961 tgtttttagg aaagccagat ttttcccaaa tataatttgt aactacatca acaggattag
     4021 atatgactac aaatatacca tcaaagccag aatcaactat gggttttact atagatttta
     4081 tcattccaat agttggtctt agtaaatcta atctagttgt gttttgtcta cgtaaaggcc
     4141 cttctgaaga gtctgcaaca cttatcacta ctatgtcaga atctttacag tcagaaaaag
     4201 ttcctttttc tatatgtact ttatgtggca gatatgaaac tgcatcagct aaatctaaag
     4261 cttgagcttt tgatttagat tcatctatat caatcataaa gagttcatca caaactcctt
     4321 gagttattaa actaaatcca caatgagaac caacatgccc tgaacctaca atagatattt
     4381 ttcttggctt tattgccatt ttaaatcact ccccctaaaa taacgataca ttgaaaatta
     4441 tagatgttat taaaagctgt ttttgaatat tttaatattc tattatttca gaaaatttat
     4501 ttaatatgat tttatactaa aatttttaaa aaagcaatgt taatatactc tctattagtg
     4561 aatattaaag tattatatgt atattaataa ttattaaaat atgtaatgtc actgatagtt
     4621 agaatattta agtgctaaaa gatgtgtata aataataatg atgtcagctt ataatgatat
     4681 taaattgaga tatactgttt ctctaaatta aaaaataaag gagtaaatgt gttatgaatt
     4741 atcatacaaa agaagaattg gtggaagcac tgagagtagt atcttcaagt attattaatt
     4801 gtggaaaagg acagaagaaa ttttctgagg agacatctca tcatacatgt tttaagaata
     4861 ttatagaagt aatgtatatt tcaaaatcat taataatgga tgaaattagt aaaagagatt
     4921 gaagcttatt ttgcaattta acatgtttta atatacattt tattatataa gaaaataagt
     4981 aaacatacat gttataaaat attggaataa tatacaatta taaatttgtt aacactaata
     5041 tctatatgtt aaaatatagt agaagatgtt agtaaataat aggatgagtt ctattttaat
     5101 acaattaaat taagcttagt ttgaaatttg tatctaaaaa gtaaagtaat ctagaattaa
     5161 gaaaatggag atagaattat aatggaaaaa ttatactata caaatcagta tataagagat
     5221 tttacagcag aaataattga aataaaagaa gtagaaaata agtatcatgt tttattagat
     5281 aagacagctt tttttcctgg tggaggaggt cagtttggag acctgggaaa aatagagaat
     5341 gatgatgtaa taaatgtgta tgaagatgga aaaaaaatat accatgtatt agataaaaaa
     5401 cctataaaga tacataaggt aaagtgtagt attgattggg atagaagaga agatggaatg
     5461 catcaacatt ttggacaaca tgtattgtct ggatgttttt ttaaaacttt taatgcaaat
     5521 acgacaggtt ttcatttggg aaatgagtat agtacagtag atatagaagg taatttagaa
     5581 gaggataaaa ttagagagat agagttgttt gccaatgaaa ttattcgtca aaatatacaa
     5641 gtagagacat tacttccaac gaaaaaagaa ctaaaaaaga tttggttaag acgtgatttg
     5701 ccaaatactg atgaagagat aagagtaata aaaataggtg atttagatac gaatgcatgt
     5761 tgtggagtgc atccaaaatc aactggtgaa ttgagaatga taaaaataaa gaaatgggaa
     5821 aaacataagt caagtacaag aatagagttt ctagcaggta aaagggcagt ggatgaagta
     5881 ttgaagaggg atatatattt gactaaaata tgtagatatt taagctgtag tgaggaagat
     5941 gctataaatg gtattgtaaa tctaaatagt aaagtggaag aggctttaat taaaaaaaga
     6001 aggcttgaag aagtagtatc tacatatcag gtcaaagaaa tgattgaaaa agcaaataag
     6061 gttggcaata tttcggtcat taaaaaaatt tatgatgatg aagatttgaa atatgtaaat
     6121 aagatagcaa ataaaattgc agaatatgaa aatagtattg cattattagc tgttaaattt
     6181 aatgataaaa taaatttagt ttttgcatgt tctaaaaatt taaaagatat taatatggga
     6241 gttttattaa aggatgcaat aaatcttata gatggcaagg gtggtggaag taaagtatta
     6301 gctcaaggag gaggtaagaa taatggaaat ttagaggcta cacttgatta tgcatttatg
     6361 aagatagaaa aaactctata aatatattat ataaaaaacc ttagtagata aaatagatat
     6421 ggaggaataa gtatggagca acaaaaggac tacatagagt tatatgaaaa tgaagaacta
     6481 atagagataa gagaagcacg tatggatgat ttggatacaa tagcaaaatt taattataat
     6541 ttggctaaag aaactgaagg aaaagaactt gatatggatg ttttaactaa aggtgtaaaa
     6601 gcattgttat tagatgagag aaaaggaaaa tatcatgtat atacagtatt tgataaagtt
     6661 gttgcacaga ttatgtatac atatgagtgg agtgattgga gaaatggaaa tttcctttgg
     6721 attcagagtg tatatgtaga taaggaatat agaagaaaag gtatctttaa ttatttattt
     6781 aattatataa aaaatatatg tgataaagat gaaaatatag ttggaatgag attatatgta
     6841 gaaaaagaaa acataaatgc aaaagcaaca tatgagtctt taaatatgta tgagtgtgat
     6901 tataatatgt atgaatacga agtaatacat tcgtaatata aatttgtaat actgttatac
     6961 tgatgttata aagtattgat tatattgacg attatatgaa ataaactgga tattataatt
     7021 agaattttta gggggtataa aaatgggcgt tttaaaagga atgttttatg tatttttatt
     7081 tttagactta gtgtcaatat tctgtttctt tttcaataaa ggaaaaatag catctaataa
     7141 aattgtgttt aatgcaatag gagtacttac atttgtactt tgttttatgc tttttagtta
     7201 ttatcctaat aataatctta ttggaaaatt tattgcttct ctatttttta tatttggagt
     7261 tgcaggagtt atgttgaaag aaaaaaattt tttatatgct agacttcttt taactgtagt
     7321 tatagttttt tctacattaa gattgttcgt tattcaataa aatgtatttg tatgaaaatt
     7381 tattaaagtc tacacattta agtgtaggct tttttgattt tttg
//
LOCUS       DAGSWI010000172         7002 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.175, whole genome shotgun sequence.
ACCESSION   DAGSWI010000172 DAGSWI010000000
VERSION     DAGSWI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.175"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..398)
                     /locus_tag="MJF80_003505"
     CDS             complement(<1..398)
                     /locus_tag="MJF80_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBZ0282115.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANKIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDY"
     gene            619..1443
                     /gene="pdaA"
                     /locus_tag="MJF80_003506"
     CDS             619..1443
                     /gene="pdaA"
                     /locus_tag="MJF80_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBZ0282116.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(1540..2325)
                     /locus_tag="MJF80_003507"
     CDS             complement(1540..2325)
                     /locus_tag="MJF80_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0282117.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            2790..3155
                     /locus_tag="MJF80_003508"
     CDS             2790..3155
                     /locus_tag="MJF80_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0282118.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            3233..3787
                     /locus_tag="MJF80_003509"
     CDS             3233..3787
                     /locus_tag="MJF80_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBZ0282119.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            4007..4681
                     /locus_tag="MJF80_003510"
     CDS             4007..4681
                     /locus_tag="MJF80_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBZ0282120.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      5036..5132
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            5186..5362
                     /locus_tag="MJF80_003511"
     CDS             5186..5362
                     /locus_tag="MJF80_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282121.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(5549..5788)
                     /locus_tag="MJF80_003512"
     CDS             complement(5549..5788)
                     /locus_tag="MJF80_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282122.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDRKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNIDRNWCKDKK"
     gene            complement(5933..6742)
                     /locus_tag="MJF80_003513"
     CDS             complement(5933..6742)
                     /locus_tag="MJF80_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0282123.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         2554 a          998 c          758 g         2692 t
ORIGIN      
        1 ctataatctt tatgatatgg ttctaaaata actgctaatt caattaaatc aataaattta
       61 ttttttaact ttggcatatg atactcaaaa aacttttttt tcagacttaa atcatgacat
      121 ataatgaaac tatcttctac aaaatttaca agtctatctt ccaccagttc tattttatta
      181 gctatttcta aatcattttt atctaaattt ttacatactg agaatacttc tataggtacc
      241 tcaaatttgt tttttattaa tgattcatag gtatatattt tccagtcttt aaccttgaca
      301 gctccaactt ctaaaatttc tgaatttaaa caatctaatc cactaacttc tatatctaaa
      361 aaaacaacat cattcaatac acttattata ttatccattt ttatatccct tcaagttaaa
      421 ccagcctaac ttatttacaa tcatttctct ttatatttta tcatttatat acactttatt
      481 taagataatt aatatcaact ttatatctta cagtaaactt ctttcattta ttatttgtat
      541 atgtgaatat ttttaactat aaatattcaa actatattat aagaaatata ctttaaattt
      601 taaataagag gtgaaattgt gaaaaaagat agcctaaaaa aatacattat gataggagca
      661 tttgctctaa tactttttgg aattgctagt ataaatttta aatcattaga taaaacaaaa
      721 actcaaattt ctagtccaac actcgacaca catgaatatg attggtattt taaccctagg
      781 gaagatggta aacagccatc acctataaaa gaagctgatt tttttaaaaa atacggagct
      841 tattatgtag gaaatcccaa tgaaaaagtt atatatctat catttgatgc tggatatgaa
      901 agtggaaaca ctccaaaatt attagatacg cttaaaaaac acaatgctaa agctcaattt
      961 ttcgtagttg aaagttatat aaaaagcaat ccagagttaa taaagcgtat ggaaaaagaa
     1021 ggacatttag tttgtaatca ttcaaaaagc catccatcaa tggcaggtat tacagatttt
     1081 gaaaaattca aagaagaaat tacaagtgta gaaaaagcat acaaagatgt tactggaaaa
     1141 gaaatgccta agtattttag acctccaatg ggtaaattta gcgaacaatc tttaaaatat
     1201 acacaagatt taggatataa atcaatattt tggagttttg cttatgtaga ttggtatgaa
     1261 aagaaacaac ctactcatga atttgccaaa aacaaaattt attcaagaac tcatcctggt
     1321 gcaatagttt tactgcatcc aaattcatca acaaatactg agatattaga tgaggttctt
     1381 acacattggg aaaaagaagg ttataaactt aaaacacttg attatttaaa taataaaaaa
     1441 tagtatcata aatttaaaaa aactctatca tgaattatag taaaaatata gttatttata
     1501 aatactttaa ttctcgatag agttttttat tttttatagc tatttaaaaa gaattgtcca
     1561 ttcatttatc tctttgtatc ctatattatg atatatttta cctgctacat cattattata
     1621 aaaaagacat ggaacttttc cttctaacaa caattcttta ctcaaattgt aaaccatata
     1681 acttgcaagt ccttttcccc tatatctttt atctgtactt acagagacca ccattgccaa
     1741 aaaactagtt tccataccag ttgacacagt gctaatcata gtatcatcat ttctaataaa
     1801 atatgctctt acatttccct cttttaaatg aacttttctt tcttctatgt aatttttact
     1861 tactttatag tctgacctat ttaaaagttt tcctatttca tcaatatctt tttcctgagc
     1921 attttctatt ttatactcct tcatgttgga aaaatctatt tcttttattt cttttaatac
     1981 acaaaattta ttatctagtt tttcataaaa atctttgtat ttacttatta gtctatctat
     2041 tacacatttt tttccactta tgctttctac atctaaatct tttatatgat tcttcatttc
     2101 tcctatatta aaattatttt taaaactata aattataagt gttgacttat tttttaatat
     2161 tatagaggtt atatttccat cttctacctg agaccatata tcttgatatt gagagctaaa
     2221 tccataattc tcaacgtatc caattatgaa taaatttaaa ctttcttcat gtgatacata
     2281 ttgccaaaat tctttttcta aagtattatc tatttttaca atcaagcaga tatcctcctt
     2341 ctatgttaat aatgatatac cttataattt atagtatcat ttaaaatcat tatattctac
     2401 caaatattct aaatttttaa atatctatgc aatatgttcg tatttatttt tatattctgt
     2461 tactgcttca ctccaagctt cttctactga aatacctctc ttatttgcaa ttttttgtgc
     2521 taattctatt attttcatag cttttcttga tatattcata tatattcctc ctctaaatta
     2581 tcataagtta ttttaaagtt aaatttctat agtcatgtta tatatttatt tacttatatc
     2641 ttactaatta tttatgtact aaatatgaac tacaatagaa gaaatttaga acttaataaa
     2701 acataaaatt tatatacagt tcatatatat ttgtcatatc acaagacaag tgatataatt
     2761 tattatcatt ttactggggg tgatatgcta tgaaaataag agctgaatat acatgtcctc
     2821 ttgaaattgt acatgacata atcaaaggaa aatggaaaac tatccttata tttgaattaa
     2881 gacaatctaa taaaacattt tctgaattgc aacataatat ttatggaata aatcaaaaaa
     2941 tgttgattca acaattaaaa gagttacgtg attttgggat tattgataaa ataagttctg
     3001 ctggatatcc attacatgtt gaatattttc ttactaatcg tggtaaaaaa atgttaaaag
     3061 cagtggaaat aatgcaagaa ataggaatcg aatatatgct tgaacatggt caacaagaaa
     3121 ttctagataa taaaggaatt tgctacaata agtaatccta tacataccaa aaagtaagta
     3181 atttacattt tttacaatag tattataata aaaataaata ggaggtataa tcatgtctat
     3241 agctttaata ttgattgata ttcaaaatga ttattttaaa aatggaaaat gtgaattatt
     3301 tcaatcagaa gaaactgctg aaaatgctaa aaaaatacta ctgtttttta gaaaaaacaa
     3361 tttaccagtt attcatatac agcatatatc aacagataaa acagcttctt tttttcttcc
     3421 taatactcat ggttcagaaa taaataaaat tgtttttcca ctagataatg aagaaattat
     3481 aattaaacac acaccagata gtttctttga aacagatttg caaagtatcc ttgagaaaaa
     3541 aaatattact aatctagtaa tctgtggaat gatgagccat atgtgtattg atacaagtgt
     3601 aagaacagca aagaaacttc gatacgacat aactctaata agcgatgcat gtacaacgaa
     3661 gaatttatct tggaataata tagaaattaa tgcaaatact gtacatcaag tattcatggc
     3721 atcactacag aacagttttg cagatgtaaa aaacacagat aaatttttat ctaactatca
     3781 caaataaaaa catacactat gttaaaagag caagtttgat ttatttaagc ttgctcttta
     3841 atcatttaaa attagatgct ttctatcatt tttatataca taatatacgt ttatacattt
     3901 tatattcata tataactatt atataaattt taaactttac tatattcaca taaaaaatgt
     3961 agtattatag tatatataat attatataga agggggtgct agataaatga accatcatat
     4021 attgattcaa tttgaatata tagcaagaat cattactgct ggtttctgtg gggctttaat
     4081 tggatatgag agaaagaata gactaaaaga agctggaata cgcactcatt ttatagtggc
     4141 attagcttca gcacttataa tcattgtttc caagtatgga tttaatgata tagtgggtac
     4201 tccaggtatt ggtctagacc cagcccgcgt tgcagcacaa gttgtcagtg gtataggttt
     4261 tttaggtgct ggattaatat ttattagaaa tcaatcagta agtggtctta ctacagctgc
     4321 tggtatatgg gcaacagccg gtacaggtct tgccattggc tctggattat acttagttgg
     4381 tattgtttca agtatcatta ttgttattgt acagataaca cttcatagag ataggaaatg
     4441 gatgaaactc cctacagcag agcaagtttc tcttaaaatt tctaacagca ctgattccat
     4501 agaatatatt gaaggtaaat ttaaaaaatc taatattgaa attataaaca tgaatattaa
     4561 acgccttgat ggggattggt tagaagtagt tatatatact aagttaccta aaggatataa
     4621 aaaaacaaat ttattaaaat tatttagcga taatcctctt atagaaactt tagaaatata
     4681 atataaaaat tgaagcttaa aaataataat atatttaatc aaggtcaata tataaataat
     4741 tacgggatga gtctatataa aattcatccc ttgttttttg ccattcatat atattttttc
     4801 aagatatttt tgttcatcta ttatctagcc aagtagctct tgtgaactaa actttttaat
     4861 acaaaaactt caatacaaaa ctaaatataa cttttattaa acattctaca tatatagttt
     4921 gtttcttgag tgaaaaatag atttcatatt gttaagatac tgttaaatat ttcaattttc
     4981 tgacaagtat gttgaacaat aacttatcct agtgtattat tttagttgta gaataaaact
     5041 aaatcggcaa aactagagaa atttagtgac gcaaagctat agggactaac gcttataaaa
     5101 taaactatga gttatgtcag ccagttgcca aaaagatatt gttctttttg ttttatgaaa
     5161 gttttttagg gggatagtaa tatatatgtt tagagatgaa atggataaat gtacacacat
     5221 gttaactgct tatattagta gtttatatga ttattgtgat tttatagata cacagctaga
     5281 tgattttata ctagagtacg gagaaaatgt agtagaatct tgtttacatc aagtgatggt
     5341 attggtaagt aagtataatt aaatagaaat tgttttctag agaacccttt ttagttttat
     5401 ctacatttaa aaatgttatt atattaaaaa actaatctcc cctatatgca aagtttcttc
     5461 catataacaa tttcttccca tttatacttt ttcacttata tctttaataa aattcaactt
     5521 caaatattaa tatacttcaa aaattattct atttcttatc tttacaccaa ttcctatcaa
     5581 tattatcctt tatataggtt ctaactattt cgccacaatc taaacataaa tctgaatata
     5641 tagtagattg tactggaaaa gttttattta taactttatc taattttcta tctcctctat
     5701 attttaaccc aatagtttca gcatatatgc caccagttgt taaaattccc ttttcaatat
     5761 ttacacttcc acaattagaa catttcacat ttataacccc ctaaacaatt attttttcat
     5821 gctatcttat aatttaaaat ataatttgct accgatttta taacacagtg acaaatcggt
     5881 ggcaaatcaa cattttttca ttctatttac catctaatcc attgaaatat agctatttag
     5941 ttataggaac ttgaatttcc gttacaaact cctctacaat tccagtctca tgtatatcta
     6001 tcttgtaaat ttctataggg tcagcaatta tcttataatt gttttcttct ataaatttaa
     6061 acaccttctc catatacatc ttattattag aattactccc agtataattt aaagttacat
     6121 aatatccttc atcaaaaact atattatata ctttctcatt actttccaga aaacaaaata
     6181 cagatttaaa ctcattaaat atacctttat taattgcttc agtagaaaag gcagaaccaa
     6241 tattgttatt tccaagtata ttaaatctat cttcatattc tttctgaagt ttttgaatta
     6301 aaaaatcgaa gtcttcatcc ctctttatat cagcatttaa ttctaaagct tttctttttt
     6361 ctatatatac aacctctatc tcatctattt ttgtattttg aattacactt tcaattgaac
     6421 tcaatctttt tataatattt tccttatggc ttataaattc agctattttt ttatcaatta
     6481 aatcgagttc ttcatttaaa atatttttcg tagactctat acttctgtca tctaggtatt
     6541 cttttatttt cttcattgga aagttcaagc ttctaaattc ttttattaaa tttaatttcc
     6601 atatatctga tatattatac attctatatc catttatatc tctaactggt cttagaatgc
     6661 ctatttcttc ataatacatt aatgaatctc tacctattcc atatatttta gatatctctc
     6721 ctattttata gtaatctttc atttatataa taaactcctc cgaaatccta tttttatttt
     6781 aaatcaacat tattactcta taatactata gtattagaat atactaaaaa aatctttaaa
     6841 acaaggatag gaaaataaac atcctaataa ttaattatta aaatattttt atattgtaat
     6901 attaggtata gtatttataa cttcaacttt tttataaata tattttagtt ttatatagtt
     6961 ttatgtttac agctattata tccatctcta tgtaatattt tt
//
LOCUS       DAGSWI010000173         6977 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.176, whole genome shotgun sequence.
ACCESSION   DAGSWI010000173 DAGSWI010000000
VERSION     DAGSWI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.176"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..564)
                     /locus_tag="MJF80_003514"
     CDS             complement(<1..564)
                     /locus_tag="MJF80_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0282124.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINLLLLPDIEKINK"
     gene            complement(567..1034)
                     /locus_tag="MJF80_003515"
     CDS             complement(567..1034)
                     /locus_tag="MJF80_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBZ0282125.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(1048..1536)
                     /locus_tag="MJF80_003516"
     CDS             complement(1048..1536)
                     /locus_tag="MJF80_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282126.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIRQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(1550..2881)
                     /locus_tag="MJF80_003517"
     CDS             complement(1550..2881)
                     /locus_tag="MJF80_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBZ0282127.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(2895..4460)
                     /locus_tag="MJF80_003518"
     CDS             complement(2895..4460)
                     /locus_tag="MJF80_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBZ0282128.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(4629..5414)
                     /locus_tag="MJF80_003519"
     CDS             complement(4629..5414)
                     /locus_tag="MJF80_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBZ0282129.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(5581..6780)
                     /locus_tag="MJF80_003520"
     CDS             complement(5581..6780)
                     /locus_tag="MJF80_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBZ0282130.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNGWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
BASE COUNT         2423 a         1220 c          706 g         2628 t
ORIGIN      
        1 cttattaatc ttttcaatat caggtaataa tagtagattt ataataagta atactacaat
       61 taaaccaatc gctacagata atccaaaata tgagaatcta agaatcaagt aagatgctac
      121 catacttcca atagcccatc caatcgaccc aaaagtctta ataaatgaca aataattatt
      181 aatattatct ccacaaccta ttatccatgt atcatataaa ctacagcaag tatttaataa
      241 tcctcctgat aaagcaacaa gtaataagta tattataaaa gttgcagatt cagtagaaaa
      301 cataataact gatgcaattc cataaactga taaactaatt aaaacaattt ttttcattgt
      361 ttgatactta tcagctaata ctccaaataa taattgaaat aatatattgg ttattgcata
      421 actcgataat ataattcctc tttcaaacgc tgagtaaccc aattttgata agaaaggtgt
      481 gaattttgtt gaagcaaagg caaaaataat atatgagaaa aaatagaata tgataaattt
      541 aaatttaatc gctttattaa tcataatcaa tcaaaattga taactttaat accactattt
      601 ccttcctgat ttaagtttga aagatgatat tttaccgtat taggattctc tacaataact
      661 aaaggaatat ataaagtatt ttcaagtaac ttattatcaa tttctactaa cttttccccc
      721 atattaactt cttgaccttg tttaacttct aatttaaagt tttctttttt tgttgttgca
      781 ctatccaatc caatatgaat aagaatttga acatcatttt gcaatttaat tcctatagca
      841 tggttagttt gagcaataaa acttattact cctttagtcg gtgatttaag aatattgtcc
      901 tttgctatca tagcaaatcc atctcccatt accttagttg aaaatacttt atcaggaatt
      961 tctccaagat taattatttt tccatcaatg tatgaattaa ttgtaattgc ttcttttttt
     1021 cttttaaaga acattataga cactcctcta cataattaac tttattctaa ttatcattac
     1081 aaataacgat attaagcata ctgcaattga ggaaaaaaga gccttatagc tctttcccca
     1141 ttgtagttct ttaatactgc ttatgatttg tctaattcca acaaatttat aaatataggt
     1201 aaacatgata acaatagtta ttgtgtaaat ttggaatatc tgccctaata agactaaata
     1261 gaaagcaatc atgttttctt ttatctcttt attctttttt atattgtaaa taacatatgc
     1321 actgtatact gcactaaagt tagagattaa atttaaactt gcattaacat cattgtttaa
     1381 aagttgtgaa aatgaaatat ttgtaaataa tgatgtcaat tctaaataca ctatatataa
     1441 aacaggtaat attagaaaac aaaccataac cactgaaaga aatatatttt catcttttaa
     1501 tactgctgtg atatttttta ttttatttgt tatcatgttt ttgttctcct tatttaaaag
     1561 ttggccagaa atccttatta gccacaatca tttcatctaa gatttctttt gctacttttg
     1621 cacttggaac agttttactc atagtaaacg actgccataa tttttgataa cttttttcta
     1681 tccatgcttc tacagctaat ttttcagaag caacttgttg ttccattaaa cctttttgga
     1741 aagttggaat tttacctata gacataggtt caaccatatc ttttccaaca aggcaaggaa
     1801 tttcaaccat tgcatcatct gcaaaatttt caatagctcc attattagga acaatcagta
     1861 aaaatctttc ctgagtatta aatgctaatg cacaagctaa ttcaacaata aattgagaat
     1921 gttcacttgc atcaaaccat gtatctcttg ctgtgccatt ttttataatt ctttcacatt
     1981 cttcgaatgt atccttttct cttctatcca taatttcatt tgctcttgta taatttggat
     2041 tagcatgttc aacagtttca tcagccatta aataatattt taaataagta ttaggtaatg
     2101 tagtcggttc tatttccaat aaatcctttg ctgctctcat agtttctaac caactagatt
     2161 ctttgtgttg accttgaggt gtaaattcct catatccttt tttagcacaa tattcttgta
     2221 atttaggcat taaatcattt ccttctttgt ccttaatact tgtccaccac ccgaaatgat
     2281 tcaatccata atatctaaca tcaaagtctt tacgagaatt taaacctaaa actctagcaa
     2341 atatttcctc taaacatact ggcatatcac aaatattaat aattcttgaa tttggtctta
     2401 atacacgaca tgcttctgca actatagctg ctggatttga ataattaagc atccaagcat
     2461 ttggagaata tttttccata taatcaatca tttcgattat tggacctatt gttcttaatc
     2521 cataggcaat tccacctgcc ccacaagttt cttgaccaac aaccccatat tttagtggaa
     2581 ttttttcatc ttgttcacgt aaagcatata aaccaacacg aatttgtgcc attacaaaat
     2641 caatatcagt aaatgcttct tctggatttg ttgtataact aaatttaatt tctggatttt
     2701 tctctctaac aattattgca catgcttcag caacttttct ttgtctctca tcattattat
     2761 catataattt aattgattca ataggaaatt tagcaacact ttcaattaac attgttacaa
     2821 ttccagcagt ataagtacta cccccaccag caattagtat cctctgtctt tttctttcca
     2881 tctttccttc tcctttataa taaattttca aattcttctc ttacacttgg tactgaaagc
     2941 ccaataataa tctgaatagc ttttccatta tgcactaaac catgtgcacc tactgcctta
     3001 aaatcttctt ctttagaaat aattgagtca tctttcaagg ttaatctcaa cctcgtagca
     3061 cagtttgtaa catctacaac attttctgct ccaccaacta attttatgta ttcagatgct
     3121 tgttgagagg ctttactcaa tttatttcct ttttcatctt tagtttctaa gtattcattt
     3181 ttagtatata gttttgtttc ttcactatct tcttctctac caggagtttt catatcaaac
     3241 ttcttaatta agaaagtaaa tacaaagaac cagataaatg aaaatataat ccctacaaca
     3301 acttgaacaa catatacctt ccagtgatac attccaagag gaagccaatt taatacaacc
     3361 cagttaataa ttcctccacc aaaatcacca acaactccta aggcaaacga aatcgtttgc
     3421 aagcacgcat ctaaaaatgc atgtactagg aataaaagtg gtgctacaaa taaaaatgta
     3481 aattctaacg gctcagtaat tccagttaat actgcagtaa gagtagcagg aatcattaaa
     3541 cctaatactt ttttcttttt ttctggcttt gcagttacat agaaagcacc aaatacacct
     3601 aatgaaccaa acaccttcga taatccggat aatgaaaatc cgattggata catttccttt
     3661 agagtcttag ctgaagtttg aaagtcctga atatgagtag cccaatatac tgatgtacca
     3721 cctggaacaa caacactatc ataacaaatt ggtgcatata caaaatgatg taatccagtt
     3781 ggtattaaaa tcttttgtaa gaatgcataa caccaaactc ctatatttcc actatttata
     3841 aaaaacgttt gcaaatgtct aataacattt tgaatatgag gccaaataaa acaagttaat
     3901 aatgcaactg gtatcataac aaagaaacaa attgcaacta caagtgatga gcctctaaat
     3961 aatccaataa aatctggtag ttctttatca taaaatctat tatgaatata aatgacaata
     4021 cctgatatta acaaagcacc aaccatacca gtatctaatg ttttaatacc agcaatgctt
     4081 acaagtccac ttccagcact tatttcagct gcgaaatcaa ctccaaagct ttttccccaa
     4141 aaacttaaca tagaacctac aaaataatta aaagtcaaat acgttgctaa agcctccata
     4201 caagctcttg cttgttgctt ttttgcaagt gctattggta aagaaatagc aaataataat
     4261 ggtaattgat taaataccat ccaagctcca ctagatatta caccccaaaa ttggtaccaa
     4321 atattttctg gtgtagcaag tgaacccata attgactgat tttgaaatac aattgagaat
     4381 ccaaccatta tcccagcaaa agcaaataat agtgttggtg taaacattgc ccctccaaaa
     4441 cgttgtattt tcttcatcat ataaattact cccctcctca tatttttagt ttatctttgg
     4501 ccttagatat ctattatttt aacaggttaa taaatatata caatctttag ggtacattcg
     4561 gagcttattc acagtcaaat aagtttttgc acatttttat gttcaaattc tcataaaaaa
     4621 aagaatgact acaatttgca atcaatccct tctgtaatca ttctatgcct tttataaata
     4681 ctatatttaa taaataataa ctcaatcaat ataaaatata acattgtcaa tttataacta
     4741 gggtgcattt gcaaaaatga gatattagtc gctgaaatat aaattgattc atcacttaaa
     4801 tcagtcaatg tattattttt aaactctgta attgaaatta ttttagttcc tattaatttt
     4861 aatcttctag caatattaag tgtattctca ttctcaccac ttagagatat aataatcata
     4921 acatcatttt tactgacaat attgtaaaaa gaatcatcaa atgttcttcc ctcaaaatca
     4981 tatattattt cttgacatga taagaacatt ctctttatct gttttgcaac cacattttga
     5041 atatcacctg aaccataagc gtatattgtt cttgattcat aaagaagttt acacaatgca
     5101 tcataatctt tatttttttg atcttcaaca tacttaatta ttgaattaca tgcaatatca
     5161 aatgactgat tttctatgac atgaaccttt tctgattccc agcttaataa tactttaaac
     5221 tcactgaaac cttttaaatc taatttttta acaaatcttg taatagttgc acgagacaca
     5281 ttacatttca tagcaagttc atttattgac atcttactac aatgtttttt atttgcagtt
     5341 atatatttcc atattaataa atctgtatca ttcaatttat tataatttaa aaaaatcatt
     5401 tcttctaatc tcataaacta ttaccccctt taattaaaaa tcgtcataaa tattatacca
     5461 atattcaaca ttaaattaca gcgtattctt atatttacta tttagcccat aaaattgact
     5521 ataaaaaata ctgctataat tgatgtttaa aaaaatgtag ttttaatttt attcttttat
     5581 ctaataatta atattttcaa gttctagtgc ttttcttata caattatcat ctttaaaatc
     5641 agcagtcctt ttacaatctt ttactttaaa ttctggaata gttttaaatt cctcatcaca
     5701 tattccattc ttatttaaat acatacaact tgccattcta actattagac cactattttt
     5761 taaattaaac aatacagggt ctatacaccc tccatctccg ccagtagttt caccaactaa
     5821 agtcgcaaat ttattttctt tacaaaacat tgcaaatgac tctgatgatg aatatatttt
     5881 tctatcaaca agtaagtata tattaccctt aaattttgaa tcatctgtag attttatagt
     5941 ataggaagta tcttcaaaat cattaaaatc ttttataatt tctttaggtg cattttcctt
     6001 tacacttgct ggtaaatttt ctacaggatt taattttata tttcttgcat ttgtatattt
     6061 ttttactatt tcattattat ttctataaat tctataacca tttattttga catcattttt
     6121 tatgagttta gatacgattc cctgccaata actatcactt ccacctaaat tacctcttat
     6181 atcaataatt aaagctttat gtttttctaa agtgttaata taatctccaa ttatttttaa
     6241 atcattattt atagagccat tctttgatga cattgatggt aaatacatat atccaatctg
     6301 acccttaata acatccttta aaattaattc tttttttaca aaaatatcat ctggcatttt
     6361 tattttaaga ttcatagagt catatctatc cacaactttt ttatcattaa gaaaatcata
     6421 ccaaccatta ttagagtatg atttcttaaa aagttcatat cttttcttat tatctattac
     6481 ttctgtgtgt ttattattta aatcagataa tatagatgac agttcttcta taaattcttc
     6541 atcattttca gtgtttttta ttcgttttgt atattcatcc ttatttgcta accaatcaat
     6601 attattaagc ctcttattta cacctagata tggatatcct gtctttacaa cattatacac
     6661 ataattaaag tcatccacct tttcctcttc agacaactga ttcttttgag aataagtaga
     6721 attacctaat aagattataa atgctaaaat aattactaaa aataatactt tctttttcat
     6781 gtaattctca tccactttca gattttatat tttaatacat tgtatcatta ttttattatc
     6841 acctaaacag ttaaaagaaa ttcttgaaga tatattccac tattattcta tttaaaaatt
     6901 tttatttgta ttttcaaaat tatttcatga aagttataaa ataacaatta tattcaaata
     6961 tctattttac tgctata
//
LOCUS       DAGSWI010000174         6971 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.177, whole genome shotgun sequence.
ACCESSION   DAGSWI010000174 DAGSWI010000000
VERSION     DAGSWI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.177"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            230..1390
                     /locus_tag="MJF80_003521"
     CDS             230..1390
                     /locus_tag="MJF80_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBZ0282131.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1732..1893
                     /locus_tag="MJF80_003522"
     CDS             1732..1893
                     /locus_tag="MJF80_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282132.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2086..2715
                     /locus_tag="MJF80_003523"
     CDS             2086..2715
                     /locus_tag="MJF80_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBZ0282133.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2954..4213
                     /locus_tag="MJF80_003524"
     CDS             2954..4213
                     /locus_tag="MJF80_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBZ0282134.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKGITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4471..5466
                     /locus_tag="MJF80_003525"
     CDS             4471..5466
                     /locus_tag="MJF80_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBZ0282135.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQFKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5712..6779
                     /locus_tag="MJF80_003526"
     CDS             5712..6779
                     /locus_tag="MJF80_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBZ0282136.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
BASE COUNT         2762 a          677 c         1197 g         2335 t
ORIGIN      
        1 attttagtaa taaaataatt gttgaaaaag attttaaatt cagattattc atttctaaag
       61 taattaatgt gcaaatcttt attaatatat gttaatagtt aggtatatat gtatttaatt
      121 agtatgatat agataaaagt atttatttta attgtctatt atctgaaaaa gtctttactt
      181 agaaatcata aaaatcaaat taattaggaa aagaaagggt gtgtataatg tgattgagac
      241 tggatttgta tattgtagtt tcttggttgt attcgtaggt gtaatttttt ttatggttga
      301 taaattgaaa tggaaggtat ttgatgttat taatccaatg cttttaatat acttaggttc
      361 agctatactt ggatcatttg gtttatttgc acagaatgaa gatgttggaa cgtatcaaaa
      421 cataataaca gtgaacttcc ttccattaat gttagtgttt ttgttaattc aatgtgattt
      481 aagaaaaata ttaaagatgg ggcctaaaat gttattatca tttttctgtg ctacatttac
      541 atttatgctt ggatttgtta tagcattcat aatttttaaa aattcaatgg gtgcagattc
      601 atggaaaatt cttggggcag catctggttc atggataggt ggctcatcaa atatgattgc
      661 agcagctaat gctctaggag ttgcagaaga aggacttagt tatgcaatac ttatgggttc
      721 tataggatat acagtatgga tgtctgtttg tctaatgtct gtgaaatttg gacctaaatt
      781 taacaaatgg acaaagtgcg atacaagttt tatagatgag gttacatcaa aacttgaaaa
      841 agaaaataaa gataatacgg cacctacgtt tgtagaactt atgattttac tttctgttgg
      901 gtttggagtt gcgtcttttg cacaaattgt atccaagata cttccaactt caggtatact
      961 taatgaaact atgtgggtag tacttgttgc atctacagta tcaatattat tgggtatgac
     1021 aaaaatatca aagcttaaag ggagtacact ggtaggaaat gtgtttatgt atttactcct
     1081 tgcagctaca ggtagtaaag caaatttctt tggattgtca gaagcacctg tttacataat
     1141 gtttggattg gtagtagtcc ttgtacatgc tatttcattt atagggcttg ctaaactatt
     1201 taaacttgat ttatttactt gtgaagtagg aagtatagct aatataggag gagtaactac
     1261 agctccaatg attgctgggg ttcacaatcc tgtattaata ccagtaggtg tacttatggg
     1321 gcttctagga aatattatag ggacttattg tgctcttatg gtaactcaaa tattacatat
     1381 gttagcttaa taaaaatatg gtataaaatt aataatttaa ataagaaaag tcatttcaat
     1441 ttaagcagaa gcttttactg aaatggcttt tttgtttgac aagataatct atttcaagaa
     1501 gatgttttga catatttagt gtataatatt gtaaaattta ctaaaaacga gatatattta
     1561 atttttatat tgaatcgaac gtctgtatga tgtataataa atatacagaa cgaatgtttg
     1621 cagaacgggt gttttttgaa tttattttaa taatttatga atatatatca gaatgcataa
     1681 atatatttaa atataaagat taaaaagaat ttaaaggagt gttgataact tatgataatt
     1741 ccatcaagaa gaatacaaga tataacatta acaaatttaa aaaatggtga agtaacttta
     1801 attgaattgg atgaaatata caagaagatg ggatttttat ttgttgttag tgaaggcaaa
     1861 ttgaaaaaga taaaaaagga gaatcatcat tagaaatgaa tgaaataata aatattataa
     1921 tagtttataa aacctaactt atgtagaata taaatactta agttaggttt ttttgtgata
     1981 aagtttataa gtactcaatt ttataagata aataatggtc ttaagtgaaa agtaaataag
     2041 tttaaatttt agtaagtatt atattgcata agaggtgata ttattatgaa aatagtaacc
     2101 tacaatattc ataaaggtat ggattcaaat aatagattaa ctctaactaa aatggggtta
     2161 tatttaaaac aattggattg tgatgtaatt tgtttgcaag aagtattata tcctcaattt
     2221 ttagctttaa aagctgtttt aaatatggat ggtgtctttg cgacaaatgt aaaaaaagta
     2281 aatatgatat atggtatttg tacatttaca acatctaaaa tgttaaacag taatcatttt
     2341 ttcttaacta gcaaaaaaga acaacgggga gctctttgta taactataga tgcatatgga
     2401 agaattataa atgtaataaa tactcacttg ggacttgata ggcaggagag agcaaaacaa
     2461 ttagacgaaa taatagacta taggaacaga ttagtgggga tagttatttt atgtggtgat
     2521 tttaatgaga aaaatgtctt tttaagtatg tttaatgata tggctatttc actaaacaag
     2581 tcttacttac caacatttga aaaatcaaac tctagaatag attacatttt tgttaataaa
     2641 aatactgaac taaaaggata cacagtagaa aaaatttacc tgtcagacca ttatcctgta
     2701 ataggttaca tttaaatttc aatataaatg aaattagttg ttataaaata aacaattttt
     2761 tgttaagaaa tagtaaaaga aatataataa aaaatatcaa aaataaaact aattataaga
     2821 cataaattta caataaagtt acatttgatt aaattccttg tttaacaaaa gctattcaaa
     2881 tataatctaa ggtagttttg aaatgggaac tctattatgc aaatttaagc tagtgttaaa
     2941 atgaggtgaa gttatgggca gaagaacaac aagagtttca agattaggta aagaaaaaaa
     3001 acgaagtgtt ttaattgtat tggcagcttt agttctaata atgggtggat ttacttattt
     3061 ttttaacagt aaatttctgt acaatgggaa aatcgctaaa aatgtatata tagaaggagt
     3121 aaatgtatct gatatgacta aagcagaggc attaaaaggt ataacagata agtatactcc
     3181 agaagactta aatcttgctt atgatgggaa aaaatataca atatcaccaa aagatattga
     3241 tttaaaatat gatacagaag atgttgtaaa agatgcttat gaaagtacaa aaaaagggtc
     3301 ctattttcaa aacttaaaaa aatatattga tataagagtg aataaggcaa atatgaagat
     3361 aaaagctgaa tatgatgagg caaaacttag ttccaaagtg tcttctatag cagatagtat
     3421 aaatgttaag atgaaaaatg cttctattag tgttggaagt ggtggactaa gttatacaga
     3481 ctctgttgta ggtagagaat ttgatttggc agcaaataaa gagtctatat ataatatgat
     3541 aaaaaataaa gagcataaaa ctttagaact taaagtaaat cttcaaaaac cagatataac
     3601 aactgaacaa gtaaaaactg taaattctgt tataggtcaa tattcaacta cttattcaca
     3661 agctgtagag ggtaggtcat ataatgtagg attatcagca agaaaaacta gtgatgtatt
     3721 attaatgcca ggtgaagaat tttcatacaa taaacttaca ggacctagta ataaagcaaa
     3781 tggttacaaa gatgcacctg ttatagttta tggtaagcta gaacaatcag caggaggagg
     3841 tgtttgtcaa acatcttcaa cagtttataa tgctgctctt ctttcgggta tggagataac
     3901 tcaagttaca aatcattcat ctgcatctac ttatgtgcct aaagggagag atgctactgt
     3961 gagcgatgga ggattaaact taaagtttaa aaatccatat aagcatcctg tatatattaa
     4021 aaattatgca ggtggaggaa gtgtatcatc agtaatctat ggtaattctg gtgacaaacc
     4081 aaatatatct atagaggtaa aacaaactgg tcagaataaa tattctacat atcgaatatt
     4141 taaagattca agtggaaagg tcataaaaaa agaacatata tcaaatagtt cttataaaga
     4201 attaaaaaaa tagataattt tttagagaaa tatttgtaat ataaaaggga gtgtctcaaa
     4261 atgaactttt tagtttagag acactctttt tatgtgaaat caaatatatt ttcatcattt
     4321 caacaataca aaagaagcgt atctctattt tgagacagcc ttttttgtaa aaattaacac
     4381 tattagttct tgttttaata ttacaatcat taatatatag aaaatattcc tatatattgg
     4441 tataattgta aatagattta gttatataaa atgtatgagg aggtgacatg gttgaatttt
     4501 ataggcaata gatatgaggt agtaaattct gatgacgttt tagaaattaa caaaatctat
     4561 aaagctagag atgtttttta caggaaaaat gtgttgataa aagttataaa gcacaataat
     4621 tatatctgtg aagattttgt ttcaaattta attgatgaaa gtactgcttt agatgagata
     4681 aactctcctt atatactaaa aataatagat gttgggattc actgtacaga agaaacaaca
     4741 ttatattatg ttgtaagtga agactttaat ggtatcggac tagatgaatt aattctaggt
     4801 aattatcttc atttagaagc aattgtaaac ataatgatac aagttttaaa agctttagaa
     4861 atgatacata ggaatcattc atatcatggt agtttaaaat cgagtagtat aatagtagat
     4921 acagaataca atataaaaat ttgtgatttt ggaattacaa aagcaaataa tggggttaat
     4981 acaagaagct atggaaacag gagatattta tgcccacatc aactatgtat aaattatact
     5041 gataaagaaa gtgatttttt tgcaacaggt cttattctat ttgaatctat atttaaaaaa
     5101 ttgccttttg gagaatcaaa ttcagaagaa aaaatgctac aatctattga taagggatta
     5161 gattggaata gtataaaagc aattaatgga aatactgaac ttataaatgt tataaaaagg
     5221 cttcttggaa gaaataataa gtataccaga gccaatgata ttattattga tttaagtaaa
     5281 gtaatgtatg aaaaagcata tatagaagtt gaagatgaga aagaagaaga gcagaaacaa
     5341 gacaaagtta tacaatttaa aaaagaatat aaaagtaaaa tgagaaaaaa attacataaa
     5401 aaacttgttg ttgccggtct agctatagtc atgatttcaa tgattattat tagttccata
     5461 atataaaaat tgtatattaa gtattttaaa atcaatgtct ataaaggata tacatagaaa
     5521 ttttagtgta gtcttaaatt atgaaaggaa gtggttatta tgttaacaaa aaataatgag
     5581 gtattaaacg tctaggagta ttttaacaaa tagccacctg cttttacagt ttagaataaa
     5641 aaagctgtgg ctaaaggtca cagctttttg ctacttttta aaggcaggag aatattaaaa
     5701 aggagcaaat tatgagcaat ataaaaataa tagcaagtaa taaatgttgg attgaagata
     5761 tagcaaggaa acaacttgaa gatatttcaa agttaaatgg aattttaaga gtagtaggat
     5821 tacctgactt acatgcaggt aaaatacctg taggtttagc agttgagact aaaaatatta
     5881 tttatccaca tataattgga aatgatatag gatgcggaat gactttattt aaaactgggg
     5941 ttctaaaaaa gaaatttaaa aaagacaaat ggattaagtc acttagtaag attaaagatt
     6001 tgtctgatat agaaataaaa aacacatata aagaagaatg tccaatttta aatttaggaa
     6061 ctatagggag tggaaatcat tttgtagaaa ttcaatgtat aagtgaaatt tataataaag
     6121 aacaatttga acaattaaaa ttttcatctg atgatattat gatgttagtt cactgtgggt
     6181 caagaaatta tggagaagat attttaaaaa aattttatga taaagatgga cttgaagttg
     6241 aaagtgagaa agctatagat tatattaaaa aacatgataa tgctttaatg tgggcagagc
     6301 gtaatcgtga agctatcagt aaaaaaatta tgcagtcaat aggtacatct ggagatgttg
     6361 aaacaatatt ctctataaat cataatttta tagagaaaag ggaagataaa tttatacata
     6421 gaaaaggtgc tgtttcaagt gaaagaggag ctgtgattat accaggttca agaggcagtc
     6481 tatcatatat agttatgcct actgaaaata cagagatatc tttatattct ctatcacatg
     6541 gagcaggtag aaaatggtct cgttcagttt gtaaatctcg ccttaaaagt aagtatagta
     6601 aagatactat aaaacaaact aaatttaaaa gtcagattat atgtaatgat gtaaatttat
     6661 tatttcaaga agctccagag gcatataaga atatagagca gattattgaa agtttgatag
     6721 aatatgaact tattcaagta gtggcaacta tgaagccatt gattacctat aaaggataat
     6781 ttttaaagtt aaaaaagatt aaaattatat tataaatagc taattttatg gaacatttaa
     6841 aagtgtttta ttattagcta tttttttatt ttgtagagac gataatttat agagctaaat
     6901 tctttgatat ataatgatta caaagttaga ctgtattgga aaatgcacat acatgaattg
     6961 atgacaatat a
//
LOCUS       DAGSWI010000175         6782 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.179, whole genome shotgun sequence.
ACCESSION   DAGSWI010000175 DAGSWI010000000
VERSION     DAGSWI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.179"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(287..1183)
                     /locus_tag="MJF80_003527"
     CDS             complement(287..1183)
                     /locus_tag="MJF80_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBZ0282137.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHKEVSNVILKEDMK"
     gene            1324..2751
                     /locus_tag="MJF80_003528"
     CDS             1324..2751
                     /locus_tag="MJF80_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0282138.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2947..5541)
                     /gene="clpB"
                     /locus_tag="MJF80_003529"
     CDS             complement(2947..5541)
                     /gene="clpB"
                     /locus_tag="MJF80_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBZ0282139.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5578..6174)
                     /locus_tag="MJF80_003530"
     CDS             complement(5578..6174)
                     /locus_tag="MJF80_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282140.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(6191..6595)
                     /locus_tag="MJF80_003531"
     CDS             complement(6191..6595)
                     /locus_tag="MJF80_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBZ0282141.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         2174 a         1095 c          710 g         2803 t
ORIGIN      
        1 cttatttaat agttttattt ctttgattaa atatctttca gaatctaagc cagatgtctc
       61 aaaaaaactg atttataata taatcatttt ttctgctatt cttatttttc caattttagt
      121 ttcatattaa aatatacttt tcatagacac tacattcttt catactcatt ttcacattta
      181 actatacaag tagtcttact catttatgta ctatacttta ttaatattta ttaagaaaaa
      241 ctcaaagatt taaaaatata tattttctat aaagtatatt tatgaattat ttcatatctt
      301 cttttaaaat aacattacta acttctttat gttcatttct atacttagtt ggagatatat
      361 tataattcct tttaaataag cgactaaaat aatttatgct atcaaagcca catctcatag
      421 caatttctgt catatttaaa tctgtttcct ttaaataata tatagcttta tcaagcctga
      481 ttccatttat aaaatcgatt gtagtcttac ctgttatttg cttaaatgtt ctacaaaagt
      541 gatgtgcact caagttcaca tgactagcaa gttcttttaa tgtaatttta tccatatatt
      601 tttcttctat caaatcaaag atacattcaa atttttctaa agaatacttt ctttttataa
      661 attcatcttc agaatatatt ttatcaatat gacttcttaa taagagtact attaattgat
      721 agatattagc ttttatagat aactcatatc caacttcttt attgtgatat tcatcaagta
      781 tagttgtaat acattttaaa atcagagaat cattttgaat taaattatta aatacaatat
      841 gatttaaagc caatggtgct aagtacttgg tttgtaacaa atcaacttga ttactaaaaa
      901 ggaatgtggg ttcaatacga atactataga attttaaatt atctgataag ctttctaaat
      961 aatgtaattc attactgttt gctattacaa tatttccagg cactacctta aaattatcca
     1021 atccacattc aagatatgct ataccatcaa taaaataata tatttgtaga tgctcatgcc
     1081 aatgtctttt aaagtttgct ccttttttaa tgtcatcctt taaaaaaata tctataaagt
     1141 tttcttgacc tttcctgtta aaatatgtat ttttatcctt cattattccc ccctcatcaa
     1201 aatagtgcta attataagca atatagttat tgtattcatt atttcatatc gctattatat
     1261 aaaatgtaac tatcattgta aacattaaca taatgcagtc attataataa ggaggtaata
     1321 aaaatgacaa actcttttaa aaaagatgat aatagaaaaa aaatcacagt tttatttgta
     1381 gtcattgcca tgacttttat ggcaacatta gattctagta ttataaatgt tgctctccca
     1441 gttcttgcaa gcaaactaaa tgtatctcta gcatctattg aatgggtaat agcaagttac
     1501 tcaattatta tttgttcaac actattattt tttggaagac ttggagatat tattggtaaa
     1561 tcaagagtat ttcaagtagg tactattctc tttacatcag cttcattact atgtggatta
     1621 agtaattctt taactttact tatagtttgc cgctttattc aaggaatagg cgcttctgcc
     1681 tacatggcaa acaatcatgg aattatcaca gaactatttc caaaagaaag cagaggaaaa
     1741 gccctaggga ttttagttac tgcagttgct attggaaata tggttggacc atctgtggga
     1801 ggatttattt tgtcaatatt tgattggaat gttatatttt ttataaatat tccaatcggt
     1861 cttattgtaa tctttttaaa tacaaaattt cttcctaata gtaaaaaatc ttcagaaaat
     1921 atggataaaa ctggtgctat acttcaattt ttaggaacaa cattattttt tagtgctcta
     1981 attagtgctc aacaaacagg attactaaat ccatatatat taattgcact attattatca
     2041 attattttta ttatattatt tttgatatta gaaaaaaaac atcctcaacc gcttcttgac
     2101 cttgaaattt ttagaaattt taaattttca ttgaatttaa tttgtgctct tacatcattc
     2161 atttgtattg catcatcaag tattttaatt ccattttatt tacaaagtac tatgaaactt
     2221 ccccctattc aagcaggttt atttatgata ctatcacctt tgattttagc cattttttca
     2281 cctatatttg ggaatatatc agataaaatt aagtcagaaa aaattatttt aattggtctt
     2341 ctcgttatga gttttggatt ctttctaatg tcaagattga aagaaagttc tgcccttatt
     2401 ttatttgtaa tttatatatc gataatatca ataggacaag ctatatttca acctgctaac
     2461 aatgctttga taatgtcaag ctgttctaga agtaagctag gagttgtagg aagcataaat
     2521 tcccttgtaa gaaatttagg acaagttata ggtattacta tttctacaac acttttatat
     2581 aattttatga gtattaaagc tggatatagg gtcaatgatt atgtgattaa taatgataaa
     2641 atatttgtat ttggaatgag aaatgtttat attattgtta cacttgtttg cttaataggt
     2701 gcaatattaa ttggctttta tttatttaaa tataataaaa atgaacaata gtttttataa
     2761 ttatattcaa attatgatta tattataaga aatttactaa aatacttcct ttgagttaaa
     2821 tttaatttgg acatattgac ttaaaaatgt tttattaatt tgataaaata tttcttttcc
     2881 tatagtttat aaataaaaat cagctgagca aaatatttag tatattatgc tcaactgatt
     2941 cttttattat ttaactaaaa cttcaatatt ttcatctttt ccatctataa ctatagtaca
     3001 tccattatac acatctccag caataagttt ctttgcaatt atagtttcta atgtatttcc
     3061 tatatatctc ttaagaggtc ttgctccata tacagggtca tatccttctc tcaccataac
     3121 atcttttgct gaatttgtaa cttcaatagt aatatctttt tcttttaatc tattttttaa
     3181 atctttcata aatatatcta taattttctt gatattttct tggtctaaag gcttaaacat
     3241 tataatatca tctactctgt ttaaaaactc aggtttaaat ctatgcttca tttcattcat
     3301 tactaaattg ttagtagtct ctgttatatt tcctcctgct tctaatagat aattacttcc
     3361 tatattagaa gtcattatta taagagtatt tttaaagtct actgtcttac ctttattatc
     3421 agtaagtcta ccatcatcta atatctgtaa gaacatgtta aatacatcct catgtgcttt
     3481 ttctatctca tcaaatagta ttacagaata aggagcacgt ctaactgctt ctgtaagttg
     3541 accaccctct tcatatccca cgtaccctgg aggaggtcca actagtcttg atacagcatg
     3601 tttttccatg tattcagaca tatctattct tattatatta tcttcactat cgaataaatt
     3661 tctagcaaga gtttttgcaa gttcagtttt accaacacct gtagggccta agaaaataaa
     3721 tgaaccaatt ggttttcttt catcttttaa tcctgctctt gcacgtatta ctgcatttga
     3781 aactgctgta actgcttcat cttgtccaat aactctttta tgaagttcat cttctagttt
     3841 aagtaatttt tctctttctc cttcaactag tttagttact ggtatacctg tccactttga
     3901 tacaatgcta gatatttctt cttcagtaac ttcttctttt aaaagagaat tttcagaacc
     3961 atcattcata ttttcttcgt actgctttat ttgttcttct aacttaggta tttcaccata
     4021 tttaacctct gctgctttgt tgaaatcata ttctctttca tacttttcag ctttaccttt
     4081 agcttcgtct aattgagcct ttaagttttt aatatctaat atttgactct tttctttttc
     4141 atattttgct gtcatttcat catttttaga ttttaactct gctatttctt tttgaatatc
     4201 atccaatctt gatttacttt tatcatcatt ttctttaagt aatgcttctc tttcagtttc
     4261 taatgtaaat aactttcttc taacaacatc taattctgtt ggcaatgaat ctatttcact
     4321 acgaatcata gctccagctt catcaattaa atcaattgct ttatctggta aaaatctatc
     4381 ttgtatatat ctatgagaaa gttttgctgc tgctacaata gcattatcat gtattcttac
     4441 tccatgatgt atctcaaatc tttcttttaa acctctaagt atagatattg tatcttcaac
     4501 agtaggttct tctgcaatta caggctgaaa acgtctttca agagccttat ctttttctat
     4561 atactttcta tattcatcaa aagtagttgc accaatacag ttcaattcac ctcttgcaag
     4621 cataggttta ataagattac ctgcatccat tgaaccttct gttttaccag ctccaacaat
     4681 agtatgaatc tcatctataa acagtataat ttttccatca gaactttgaa cttcttttaa
     4741 aactgccttt aatctttctt caaattcacc tctatatttt gcaccagcaa ttaaagctcc
     4801 catatcaagt gagaatacaa tcttatcttt taaaccttct gggacatctc ctcttactat
     4861 tctctctgct aacccttcta caattgcagt tttacctaca cctggctcac caatcaatac
     4921 aggattattt tttgttcttc ttgataaaat tcttataatc cttcttattt cttcatctct
     4981 cccaattaca gggtctaatt tatttttctt ggctaaatct actaaatttg ttccatatct
     5041 agcaagtgca tcatatgtgc cttctgggtc ttgtgtttct actctttgac tacctctcac
     5101 ttgtgataaa atatttaaaa agtcattctt atttatatta tattgtttta atattttacc
     5161 aacagctgat tttgattctg tttccatcat tgcaagcata acatgctcta cacttatata
     5221 tgaatcttta aattctttag aaatactctc tgcctttata agcacctcat ttatttttct
     5281 agtagctgat actccttgtg agtcagcacc ttctccatat acttttggaa gcttatcaat
     5341 ttcaaagttt acagtatttc tcaaagaatc aattgatata ttcatttttt cgattatatt
     5401 tggtatcaat ccatcctctt gattaataag tgctgagaaa agatgaatta catctacttg
     5461 ttggttatga ttttttactg cttcgttata agcctcattt aaactttttt gaactcttaa
     5521 agtcattttt tctacgtcca tataaatccc tccattttaa tattatatcc aaataaatta
     5581 aattctctca tttataatta ttgattttct aagctctaaa agactatcaa gtaaattctc
     5641 gtttttttgt atatcgaaac tcaatgttaa attaaatgtt ccatctgaat taaaaaaatc
     5701 gaatacttct tttgattttg ctattaaata caagtcttca tctataagtc ttttttcacc
     5761 tattaataaa tactgacatt tatctataaa actcttttta aaatcaatag tgtatagatt
     5821 ttctaaaata ttatattgat aaatagcaat attattaaac acttttaata tgtttgaatt
     5881 tacaaaattg taaatttgat tattgttata cagtgatatt tgttcgtata tatattccct
     5941 aaataaagga tgaatgtttt gcttaagttt taaagtaact tcagctggta tttcaaaata
     6001 aagcttccag tacttagagt tatttttttc taaaataaaa gctactttgt tcaaactggt
     6061 agaactaatc tcctttagtg tacaattaaa attatagtgt ctcaaactag ttttttcatt
     6121 ctcaaataca tagtcaaacc actgtagaac atttctatca agttttaaac tcatataaaa
     6181 accttcttcc ttatttacta accttattag gtctttttat ctgattatat tctgctaata
     6241 aactttcttt atcagctcta ctacctttat atgctatatg tggatttatc atatactcac
     6301 catttttcac tctttgtaaa aaatccattt ttttcagctc cgtaaacaat gaagataaag
     6361 aagtttttgg atattcaaat tcacaagata attcatataa tgtttttcta atcttattat
     6421 ttttatccat agagtttatt aattttaatg gaattaaatc cctcttagta tacattcctt
     6481 ctactacttt aatataattc ttaaaacaaa cattcatata cttactttta ttcataacta
     6541 aacttttgct ctctgtatat tcctgattta aatccttttt taatgtctta tccaaattaa
     6601 atatctcttc tcactctcct tatctctaat tgataatata aatatattac aaaaaaatat
     6661 gtatgtcaaa actgaaaatg caaaaagttc accttataag caaactttta tttagctata
     6721 agctaaactt tattattttt ttaatttagt atttccaatt tattggcagg atttttgaac
     6781 aa
//
LOCUS       DAGSWI010000176         6568 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.180, whole genome shotgun sequence.
ACCESSION   DAGSWI010000176 DAGSWI010000000
VERSION     DAGSWI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.180"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(236..1681)
                     /locus_tag="MJF80_003532"
     CDS             complement(236..1681)
                     /locus_tag="MJF80_003532"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBZ0282142.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(1825..2733)
                     /locus_tag="MJF80_003533"
     CDS             complement(1825..2733)
                     /locus_tag="MJF80_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBZ0282143.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            2990..3814
                     /locus_tag="MJF80_003534"
     CDS             2990..3814
                     /locus_tag="MJF80_003534"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBZ0282144.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3854..4384
                     /gene="lepB"
                     /locus_tag="MJF80_003535"
     CDS             3854..4384
                     /gene="lepB"
                     /locus_tag="MJF80_003535"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBZ0282145.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDENEVLG
                     KVMVRLLPLDNLGKVD"
     gene            complement(4466..6247)
                     /locus_tag="MJF80_003536"
     CDS             complement(4466..6247)
                     /locus_tag="MJF80_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBZ0282146.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         2130 a          971 c          795 g         2672 t
ORIGIN      
        1 agtacaatag tttttgatga atgtttaaat ttaattagta atcttatcat ttttttattt
       61 ttatttatat tattcctaat taaatattat ttatatagct attttattaa attcatggta
      121 tagtaagtaa agaagaactc ttcacagcgg tggagagaag acgctagccc attaaaatgg
      181 ctatcttatt tataaataag atagccgttt ttaattatat tttatctgta caattctata
      241 ctcttaggtc tacatctact tttcctaagt cttccttata atcttttata gcaggttcta
      301 caacttcatt tacaaattct acaacttgtt ctggagctct acctataaag ttttttgggt
      361 ccattataga tagtatttct tccttgctca ttttaaatga atcatcattc attataagtt
      421 caattaagtt attatccttg ccttcatgct ttactctata ggcagctttc atagaatatt
      481 ctcttattat ttcatgtaat tcctgtctat ctccacctct ttttacagct tccataagta
      541 ttgtttctgt agccataaaa ggaagttctt catttacacg tctatttatc atattttcat
      601 atactactaa accatcagtt acatttatcc ctatttcaag tatagcatct gctgccataa
      661 atgcttgagg tattgccaat ctcttattgg ctgagtcatc taatgttctc tctaaccatt
      721 gagtggcttg taccattgca gggcttatag attcagagat tatatactta gataaagatg
      781 ctattctctc acttctcatt ggattcctct tataagccat tgctgatgaa cctatttgat
      841 ttttttcaaa tggctcttct aattctttta aactttgtaa aagtcttata tcattagtca
      901 tcttatgcat actttgagct atccctgata gtatgcttat tacttgataa tctaactttc
      961 tagtatatgt ctgtccactt actgcatagg aagaatggta tcccatcttt ttacaaattt
     1021 tcttatctaa ctcttttact ttttcgtggt ctccttcaaa taagcttaaa aaacttgctt
     1081 gagtaccagt agtaccttta actcctctta acttcatgtt gtctattcta tagtttaaat
     1141 cttccaaatc aagaattaaa tcttgagccc ataaacaagc tctttttcct actgttgtaa
     1201 gttgagcagc ttggaagtgt gtaaatccaa gagctggcat atctttgtac ttaagtgcaa
     1261 attcttttaa attgtttatt aaatttacaa gctttactct tagaagtttt aaagcttcat
     1321 tcatttgtat aacatctgta ttatctccaa catatgcaga tgttgctcct aaatgtatta
     1381 ttcctttggc tttttcacat tgtaaaccat aagcttttac gtgagacatt acatcatgtc
     1441 ttacttcttt ttctattttt ctagcttcat caaaatttat attatctata ttagctctta
     1501 gctcatctaa ttgttcttca gttatattga ttcctaactc atgttcacct tctgcaagtg
     1561 caacccataa ccttctccat gtagagaatt taaattgtgg agaaaatata taactcatgt
     1621 ccttgctaca atatctgtct gtaagtgggt ttgagtaagt agtatattta ttatctatca
     1681 ttatattagc accttcctta aatcttttgt cgtgtgatta ctgtgtacac attagtacat
     1741 aaataattat atataatttt acgaactata acaagacttt tttatttttt gttcacattt
     1801 atatatagta ttttttattt attactattt tttatttata tccaaattat ctttttcaat
     1861 taagtaatcc actatttcac tatcaacaaa atttaataca tacttatatc taattgcata
     1921 gtatttatca ctccatccaa gctgattttt tacatcactt tcatctgccc caagccttaa
     1981 acttattgct gcaaatgaat gtctaaaatc tcttgctgaa tattgagaaa gtccagctaa
     2041 atctaatgct tttttgacta taagtctgac atttctatct gttatagaat tactcttttg
     2101 agttgtaaaa ataaaatcat cactaggtat tattacctct ggaagcccag aataattcct
     2161 gtaatcttct aaaagtttaa aaaagtatgg atgtaattta actactcttt cttttcttcc
     2221 cttacctaac cttacatatg agttatcgtt ttcatctacc ataaggtctt tccactttaa
     2281 gctaactaat tcatttaata aacacccagt agtaactaga cagactatta ttactctatc
     2341 tcttatattt aattttggta ggatacctat aagtttattt atttcttgta tacttagaac
     2401 atcatctttt gtttttattc ttgttactgt gggtttttct acatatctaa atggatttat
     2461 atctatgtat ccttcttttt ttaagaagtt ataaaaacta tgtaaataac tatatatctt
     2521 ttcacaagtt gatttcgcat atttagtctg tatatagtct acaaaatttt tacaatcttc
     2581 tttcgtagca taaatcaatt ctttcccttt taaaaattct ttaaacagta taacctttga
     2641 taaataatcg tgtttcgtgt ttggatttaa ttttgtagaa aaaacattaa aagtttcata
     2701 ctcataatca tctagtattt ttgtactata cataataact cctttccttt tcataaaata
     2761 aaatagctat acttttacta taccatatat ttccccctca aatatacccc ttattcaata
     2821 gataactttt aagtttattt tgttatatta tgaaggtttt atgaaaatat caatcttcca
     2881 ttgtgcacta cacagtacta tggtattata agttagtaaa aggtggcatt tttttattaa
     2941 tctatgaata aaatatgcta ctataaattt gtattggagg tattttgatt tggaaacatt
     3001 tagtttgctt gaaattttta aggcagtcat tttgggcata gttcagggaa taacagaatg
     3061 gctaccagta agtagtacag ggcatatgat attagttgat gagtttataa aattgaattt
     3121 ttcaaataca tttataagta catttttagt tgtaattcaa tttggttcaa tattagctgt
     3181 acttgttata ttttttagaa aattaaatcc ttttgatagc gcaaagaata ttaagcagaa
     3241 aaaagaaaca gtccgtttat ggcttaaggt tataattgca gttataccat ctggagttat
     3301 tggaatactg tttgaagatg atatagatag attattcttt aattcaacag tagttgctat
     3361 tgctcttatt gtatatggta taataatgat agggcttgaa aagagaaata aaaaacctaa
     3421 gtataaagat ttttctcaag ttacatataa attagctctt tgtataggat tattccagtg
     3481 tctagctctt ataccaggta catctaggtc tggttctact ataataggtg cagtattact
     3541 tggtacttct agatatgtag cagcagagtt ttcattcttc ttagccatcc ctacaatgct
     3601 tggagctagt gcattaaaac ttctaaaagc tggatttgga tttactggat ttgaatggct
     3661 tattttaggt gttggttctg tagtagcatt tgttgtttca attgtagtta ttaaattttt
     3721 tatggactat atcaaaaaac atgacttcaa agtatttggt tattatagaa tagtcttggg
     3781 tatagtggtt cttgcttatt tctttctatt ataatgtatt ctaatttaat aacaaatgat
     3841 aaggagaaaa gttatgagtg ttaaaaaaga aatatttgat tggattaagt caatagctat
     3901 ggctattgta cttgcatttg taattctaca atttataaga ccttctattg taagtggaga
     3961 atcaatgtat cctactttag atgataaaga ttatctgatt ttaaatagga tatcatacaa
     4021 ggttggtaaa cctgaaaaag gcgatattgt agtttttaaa accaatttag ttgatggaga
     4081 aacaggaaag aaaaaagact taataaaaag agttatagct actgaaggtg acagaataaa
     4141 aatatcaaat tctaaagtgt atgtaaatgg aaaattatta aatgaaccat atatacacaa
     4201 taactatact tctggagata tagatactgt tgttccaaaa ggtaaactat ttgcaatggg
     4261 agataataga gaaaatagta atgatagtag attccctgat gtaggtatgg ttgatgaaaa
     4321 tgaagttctt ggtaaggtta tggtgagact attacctctt gataatcttg ggaaagtaga
     4381 ctaaaaaata agagactgaa ttggtaaagt ttattttaca aatctcagtc tctttttatt
     4441 ttcttaaatt acaaatattt ttttactaaa tatcttttga ttctgtaata agtcttatta
     4501 tatcacttgc ttcttcatta aaagttgtta ctatacttag gtagtctgtt gctctactca
     4561 tagctaaata aaccttattt aaatctccta caaagtcatt tacttgataa tcttgttcaa
     4621 tatcatttat agtttgattg tataacatct ctaattcaca tactattacc gctttatact
     4681 ctaaattctt tattgcatat atatttgcta ttgtagttcc taccttctta cttatatttg
     4741 tcaaattatc ttctgcacat atataaggaa tttttgcctc ttccaatgct ttttttaaca
     4801 tatattgaaa gtaaatggtt tttccacttt ttaatttctt cttattgtaa gggtaaacga
     4861 tagcaacttc agaataatca agaccttttt tacttattaa atactcaatt tcccagatta
     4921 cagaacttat ttggtcatca aggtcactta cttttattat atcaacagac ttatttccac
     4981 ctctaagtgc tttagtataa aagaaagtac taaattctgt atttggtctt aattcgttga
     5041 tatatgagtt agaattattt gaaaatttat ttgtaaaatc tacaatctct ttagcttgtc
     5101 tataattttt acttaagata atcttatcat caaattctat accattatat aattttttaa
     5161 atatattcaa tctattagag atattcaagg agttacaaga acacacatta aatatgaatt
     5221 ttgttttata taaaaactcc cttataaaat ctatttcata ctctaaaaag ctttctgctt
     5281 catcaataaa aatagccttg aacatatttt tattttttat aatgttctgt gcttgtttta
     5341 caagattatt aaaggtcttt tcgtaatcat tttttaacat gttataatct acaattaaat
     5401 tgaatttttt agctagttta aatataaaag aactaaatgt gtgaacttct aaattgttat
     5461 tatctttgta taataactct atcctctctc gaagctcatt gcacaattgt tttgtatgtg
     5521 taaatatgac taatttatga tgtggatata cttttgctaa ttttatagcc ctagaaagca
     5581 taattgctgt tttacctata ccagaaccac cttcaataag tgtattccca tatttaatag
     5641 aaacaacatc tttaatttga acgtcttcca tcatagttgc tgtatactta tagtcatcat
     5701 tgtagaatga aattttttta aatttttcat ttagatgaag tttatcattt attatatagt
     5761 attctgaaca tatgtctagt aaaaataaat ttaagtttat ttcatcattc tcatcattaa
     5821 gatattcctc cagcgaaccc ttattcatta tatcttgaag cttattttta tctattatat
     5881 tattattaac aaattcttca aattcatatg tttcctcaac ttcaacgtat ggcataatgt
     5941 aaacgtagtt ataacttata tttctattct tttgtcccat ttttagtttc aaaagttcat
     6001 attcctcatt cataacttca agtaactctt cttctaaaat aaaaaatata tcttctgttg
     6061 tatccataaa ttttataaat aaaattcttc catctttaac atacaacaaa tcggtattta
     6121 ttcctttaaa aggagtaact tttggcacta ctttaacgtc ctcattgata ttttcgtaaa
     6181 atattttctc gaactccttc aattttaggg cgtctaactg agaatttata tttatgtatt
     6241 ggctcactta cattacctcc ttctgttata cttaattata ccattttgaa ctatattttt
     6301 atatatactt tttataggca aatttagtaa ataaattcaa aataaataca aataaataca
     6361 aaactgctaa aataatgttg caataaatat agatatttat tgttatagaa tactttctgt
     6421 aaataataaa acgataaatt tcataattaa tttttaaaat aataagtaaa gttttaaaaa
     6481 aaataaatct tattgactaa aaataagatt tattataaaa aatttaaaaa cctgctaaaa
     6541 ataatctatt ttcattagca ggttttat
//
LOCUS       DAGSWI010000177         6478 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.181, whole genome shotgun sequence.
ACCESSION   DAGSWI010000177 DAGSWI010000000
VERSION     DAGSWI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.181"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     misc_binding    277..515
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            579..2498
                     /gene="thrS"
                     /locus_tag="MJF80_003537"
     CDS             579..2498
                     /gene="thrS"
                     /locus_tag="MJF80_003537"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBZ0282147.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            3266..4357
                     /locus_tag="MJF80_003538"
     CDS             3266..4357
                     /locus_tag="MJF80_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBZ0282148.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            5027..6367
                     /locus_tag="MJF80_003539"
     CDS             5027..6367
                     /locus_tag="MJF80_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBZ0282149.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT         2569 a          596 c         1118 g         2195 t
ORIGIN      
        1 caaaacctaa aaagagtaca aaaattagaa gttagtttta ataaaaatta gttttagtat
       61 ttatctagtc atatctatca tgcatgtgga tacgttttaa aaattttaaa tttatgaaaa
      121 tattataata taactataaa tcaagagatt aatttaatat aaaaatataa gaatattggt
      181 ttacagctga ttgagatgaa aaataatggg taattaatat attacattaa tgttgattga
      241 tgtataacta tctgttataa tgaaaatata ttaaaaatca tagtgttgaa gaggaagagt
      301 agagtagaaa ccattctcag agaatttggg tctatggctg agagcccatg taataggtct
      361 atttgaaaac taccttggag ctgagcttat tagcagtatt tgtatttata ctatacgtga
      421 aagtgctcgg gtgacgacct tatagtcatc aagagggttg tatatacttg tatacgccaa
      481 cttgggtgga accgcgaatt aatatcgtcc caaacatatt tggggcgatt ttttattaca
      541 aaaaatctaa aaattatttc aataaggagg aaaaaacaat gattaaagtt gctttaaaag
      601 atggctcaat aaaagaattt gaaaatgcta tttctgttat ggatgtagct aaatccatta
      661 gtgaaggatt agctagaaat gtggtagcag cgtctgtgaa cggagaggtt gtaggtctgg
      721 accacattat agatactgat tgtgacttga atttatttaa gtttgaagat aaagaaggta
      781 aggaagtttt tagacataca tcagcgcata tattagctca agctataaaa agactttacc
      841 cagaagcaaa gcttgctata ggaccaagta tagaaaatgg attctactat gatatagatt
      901 tagaccatag attagttcca gaggacctag agaaaataga agctgaaatg aaaaaaatag
      961 ctaaagaaga tttaaaaata gaaagatttg aacttcctag aaatgaagct ttagagctta
     1021 tgaaagaaca aggagaagac tataaggtag aacttatttc tgatttacct gaaagtgaaa
     1081 taatatcttt ctacaagcaa ggtgatttta ctgatttatg tagaggacca catttaccat
     1141 ctactaaaaa ggtaaaagct gttaaattac aaagtgtagc aggtgcatat tggcgtggtg
     1201 acgaaaataa taagatgctt caaagaatat atggaacttc ttttgagaaa aacaaagact
     1261 tagaagagta cttacattta ctagaagaag ctaaaaagag agaccacaga aaattaggta
     1321 aagaattagg attatttatg atacctgaag aaggtccagg attccctatg tttttaccaa
     1381 aaggtatgga acttaaaaat gaattattaa aattctggag agaaatacac agaaaagctg
     1441 gatatattga gatagaatct ccaatcatat taaatagaaa attatgggag acttcaggac
     1501 actggtatca ttacaaagaa aatatgtata cagttaaaat agatgatgag gattatgcta
     1561 taaaacctat gaactgtcct ggtggattaa tttactataa ctctcaacta cattcatata
     1621 gagatttccc aatgagagtt gcagagcttg gtagagttca tagacatgag ttatctggtg
     1681 cattacaagg attaatgaga gttagagcat ttactcaaga cgattcacat atattcatgt
     1741 taccagagca aattaaggac gaaataaaag gagtagctaa cttaatagat ggtatataca
     1801 aaacttttgg atttgaatat aatttagaat tatctacaag accagagaac tctatgggaa
     1861 gcgacgaaga gtgggaagca gcagaaaatg gattaagaga agctttagaa gagttaggtc
     1921 taccatatac tattaatgag ggtgatggag cattctatgg tcctaagata gacttccatc
     1981 taaaagattg tttaggaaga acttggcaat gtggtactat ccaattagat atgcagcttc
     2041 caagacaatt tgataatact tatattggtc aagatggtga aaagcataga ccagttatga
     2101 ttcatagggt tgcttttggt agtatagaaa gatttatagg tatattaata gagcattatg
     2161 ctggtaaatt cccagtttgg ttatctccaa ctcaagttaa gatacttcct atatctgata
     2221 aatttatgga ttatgcaaat gaagttaaga aagagttatt tgataagggt ataagagttg
     2281 aattagatga tagagctgaa aaaattggat ttaagataag agaagctcag cttgaaaaag
     2341 tgccatacat gttaatagta ggtgaaaaag aagtagctga taataatgtt tctgttagat
     2401 caagagataa aggtgaaata ggtagtatta aattagatga atttatagct agtatttcaa
     2461 aagaaattga atcaagagaa agtattattc aagactaata tatattatat tagtagctaa
     2521 gatattgtta tataagataa gtttaatcaa tatgttgata tgttgatgca tagtgattaa
     2581 atttatgaaa agatgagtat aattagaggt acagttaatc atatttttat gattaatgta
     2641 cctctagttt tttgtatggt agatttatta ataaatatat catagaagtg ttttgaacat
     2701 ggaaatattt atgtgcattc tttaaataaa agtttatttt ttgagtgata attaagtgct
     2761 tgttggtaag gttatggctg taaaaatcat aactttattt tcatatttgt tccattttca
     2821 taaaaattat tatcccttta agaaaatttc ttaatcctct tgaaatattt cctttttagt
     2881 ctaaagttaa atttctttct tcaaaatgaa tatttaaatg aactaaaatt taagtacatt
     2941 ttctaatctt atgagtctat ttttcataga taattgccct ataatagaag aatctatgtt
     3001 ggtgctatgt ggtgacaata acagcattgt cagtgattaa ttaatgcttt ataaatgatt
     3061 aattagtgtt ttatgttatt tttggactta tcctctatac atagattaat ttatttaact
     3121 ttagatagag agagaatgtc aagtacatac acagacatta gagtttaaga aagtgtttat
     3181 ggagcaagaa cttttaaaag aggtagtgac ttagtttaat ttcaagaaat gataaataca
     3241 aaagaggaaa aagagaggtg ttgaaatgat agaaaaagta gaagaaaaat ctaagattat
     3301 taaaacatca aaagaatata agcgaactat aattgaagaa aaagataaag aatggaccaa
     3361 aaaattaaat gatatactta gtagaagtag tttttctaat gatgaattag ataaaatcaa
     3421 ggcattaatt ccaaaagaga ttttgactaa tgaaaatgtt ggagaagttg tgactgaaga
     3481 tggatatgct aagcctgagt atgatgaggt atttaaatca ttgttcactt taaataatga
     3541 atatgattta cttgtaaatt ttataaatga tattctaaaa gatgctccat atgctaacag
     3601 gaatatcaaa ccgttcactc aaataaagag aataattaga gttgaaactg atccaacaat
     3661 taattacatt ggagagaaaa gacctaaact tgatattttg gctgaagatg aagaaaataa
     3721 tcatataaat attgagatgc aaagagcttt tgagaacgat tatttggaaa gagctgaata
     3781 ttatcttagt cgtgtacatg gtagaaagtt agaagaaggt aaagagtata aagaaattgg
     3841 aaaaactatt ggaatacata tactaaatca tgtaaaatat aatcacatag aagattatgt
     3901 aaattgcctg agattaacaa tggacgggca tccagatatt tattctagta aaactgcatt
     3961 atattttata gagcttccaa aaattcataa atctgactat atagtaaata gaattatgct
     4021 atggggaaaa cttattagta atccttctaa cttagatgta agagttatag ctaagaatga
     4081 ttctattatt aaaaaagcac ttgatagatt aaaagaatta ggtagcaatg aagaatattt
     4141 attaaatctg aaaagaggtg cttatatcat gaataaaagt agaaatttta aagaagagat
     4201 tgaaagagag acagcaatta gagttgcaaa agaaaaagat tataaaataa ttataatgtt
     4261 gaaaaaaaat ggttttaaat tagatgacat attaaataaa tttaatgatt ttgacttaag
     4321 tgaagatgag ataaaagaag tatatgaaaa taattgatga taaataaaaa gtgaacttat
     4381 ataagtttgc tgaatcggag tctagaaata tttatgtgta ttttttaatg aataaaaatt
     4441 tattttaaaa acaataattt agaggtatag ttaattatat ttttatgata ctgtacttct
     4501 agttttttct attgtagatt tattatttac taataaatct acaatagaag tgcttttgtt
     4561 gtaagaatca ttttatctaa tgattgtttt ttataatagt taaatttcca tttgtagtgt
     4621 ttaaatataa atctataaca gcatttctat aaatgttata aatcactata atgattcaac
     4681 tcaaagttac tgcttaaaga agcagagatt ttatcaaggt atatcatgga aaaaaatatt
     4741 ttattgtaat atttataaga ttttattata gaaataatgt aaaaacttta taaataatat
     4801 attttatacc tagttataga taaatttata tagttattat taaaaaaagc aataaaaagt
     4861 ttaattcaat ttagcaaatt tagtgatata attaaaaaag aaaagaaata tggacttatt
     4921 tggaataatt ttgacaatct atttactaca tgtgtagagt cgtatataat taaattatac
     4981 ataaaatata aaaaaataat ttagtaatac gttaggagaa agtattatga taaagtcaag
     5041 cttattttgg aatattttca ttgcgatatc ctttatgata gaatggtttc tttgtaagaa
     5101 aatgctagat tatacaagtg agaaaaggat ttctaacaaa aaaataaact tttgtatggt
     5161 aggtattatt gtttttgctg acataatata tttttttgat gtacctccaa atataagagt
     5221 aattatatct atgataatta ctattgcatt ttataaaatc tcatatgatg tggggtttat
     5281 aaagtctgta ttaatatcct taatttattg gatgttacta atcgccacag atgctttgag
     5341 tatatcttta acagtttggc ttaattctat aaatgacatg agtagtatgg ggacgggaga
     5401 tatatacaga attgagtcta taatattggg aaagatactt ttgattgtgt ctttgctttt
     5461 atatagagcc ttaaagataa aacttaatat agaaataaat aaaaggacat tattttatat
     5521 agttattcct atagtcgcta atatagcatc tttttttgta atttttgaat acatatttaa
     5581 tccagggtct ggtagcttaa tacataattt ccaattcgtt atgatttctg ttgtattgct
     5641 tatatcaagt atcactttag ttttaattgc taaaaaatta cagcaatata atactttagt
     5701 tgtagaaaac aatatgatta aaaatacttt gaagtatgaa aatgagtatt atgaagatat
     5761 agaaaagaaa tatttaaaaa ctagattact aagtcatgat atgaaaaatc acatattgtg
     5821 tataaatgct atgattaaaa aaggtattga tgttacttct tatttaaata atttgcaggt
     5881 agaaattcag agtaatgact tgttatttaa tactggtaat tttatattag atgctatatt
     5941 aagtgaaaag aaagaggttt gtgataagaa tgatatagaa ctaaaaatag gaataaattt
     6001 tagttgttgt gaagctgttg aattagtgga tatatgtcat atattttcaa atattttaga
     6061 caatgcaatt gaggcatgta ataaaattga tagtggaaat agagttattt gtgttaaagg
     6121 tgatgttatg caaaattttt atttaataag agttgaaaat actaagataa ataagattat
     6181 tactaaaaat ggagatattt tgacagacaa aaaaggttct cttcatggac ttggaataaa
     6241 aagtgtaaaa aactctgtaa ataagtatgg tggtaatgtt gtaattgaat atgatgataa
     6301 aaaatttgta gttaaaattg ctattccact tagaaatgtg aagaaagcag ttaatcatgt
     6361 gatttaaatg tatataaatt tgatgtgtag aaatttaata tataagaatt aatgtataaa
     6421 agtgatgcat agaagtttaa tatataagaa ttaatgtata aaagtgatgc atagaagt
//
LOCUS       DAGSWI010000178         6463 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.182, whole genome shotgun sequence.
ACCESSION   DAGSWI010000178 DAGSWI010000000
VERSION     DAGSWI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.182"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            146..1549
                     /locus_tag="MJF80_003540"
     CDS             146..1549
                     /locus_tag="MJF80_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBZ0282150.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1640..1880
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1923..2621
                     /locus_tag="MJF80_003541"
     CDS             1923..2621
                     /locus_tag="MJF80_003541"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBZ0282151.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELLNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2644..3645
                     /gene="trpS"
                     /locus_tag="MJF80_003542"
     CDS             2644..3645
                     /gene="trpS"
                     /locus_tag="MJF80_003542"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBZ0282152.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4297..5864)
                     /locus_tag="MJF80_003543"
                     /pseudo
     CDS             complement(4297..5864)
                     /locus_tag="MJF80_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(5839..5982)
                     /locus_tag="MJF80_003544"
     CDS             complement(5839..5982)
                     /locus_tag="MJF80_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282153.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(6200..6382)
                     /locus_tag="MJF80_003545"
     CDS             complement(6200..6382)
                     /locus_tag="MJF80_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282154.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
BASE COUNT         2288 a          771 c          996 g         2408 t
ORIGIN      
        1 tttcaaagta tttaaggttt attattaaaa ctgtttttga atatgcaaat attttgaaaa
       61 atatcagttt aataataatg aatctatttt attaattttg aaattaaatt taaaataatt
      121 aatagagagg ggaaattaaa ttaggatgga aaagaaaaaa ataggcctat ggagtttggt
      181 atttatgaat gtatcagctt tatatggtat aagatggata gctaaatcta cagcatcaag
      241 ctttggttta ggattaggtg caatacctat gtggttatta ttctcattta tatactttgt
      301 accaggagct ttaatttgtg ctgaattagc ttctacatat caaaaaggtg atggaggtct
      361 ttacgattgg gttaaggaag cctttggaga aaagtatgga tttttagttt cttggttaaa
      421 ctggacagct aaaatatttt ggtattcttc atttttaact ttcttagcag taaatgtttc
      481 ttatacaata ggaaatccag cccttgctga taataagatg tttgtactta tattatcttt
      541 aataatattc tgggcattat ctcttatagc aactaaagga ataagtttta caaaaatatt
      601 tactaataca ggtgcattag gttcaacaat accaagtgct atacttataa tattttcatt
      661 tgtagcagta tttgtgttga aaaaacatga tatagcttct acatacacaa tagcttcaat
      721 gactccaaaa cttaatatgg atgcatttgt tgctatatca gctataatgt ttggtttagc
      781 aggagcagag actgctgcta acttcataac agaaatagat aagcctgaaa agaacttccc
      841 taaggcaata ttaatatcag ctggaatagt agcttcatta tatgtattag gttcaattgc
      901 aataacaatg ataataccac cagaccaaat tactgcttct aaaggtgtat tagatgcact
      961 ttctgctgtt tgtgcgtctt taggaattgg ttcttggttg gttcaattaa tagcttttgg
     1021 tatagctttt tcagtattag gagcaatagt tctttacata gcatcaccaa taaaaatgtt
     1081 atttggtagt gttaaaaaag gaatatttcc agatagttta gtagaggtaa atgaacacaa
     1141 gataccttct aaagcagtta tacttcaagc aataatagtt actataatac tattggtgac
     1201 tacattaatg ccttctgttg atgctatata caatgttctt gtaactatga cagcattaac
     1261 tgcattattc ccatatgtat tgttatatgc ttcttatatt aaactaagaa aagagagacc
     1321 agatgaaatt agaccttata ctatggcaaa atcaacaagt acttgcctag gtttagcaaa
     1381 aatggtttta gttgtaactg tagtgggtat attattatct gcagcaccag ttatgcctac
     1441 atttgctgaa aatgttatat atgagataga gatgattggt ggaggtcttc tagttatatt
     1501 atctggtcta tggttatgga atagatatga aaagaaaaca gctaaataaa atgtatttta
     1561 aaaatttctt ttggatatta taatatatag ttgacaattt atagtgtaag gaatattata
     1621 aaataaagaa attaataaat taaatcctga gataagaaat agtaaataaa tatatttttg
     1681 cacagagaat taaggatgct gagaacttaa taaaaagtat ttattgaatg ggtctttgag
     1741 gagtaaaatg aaatgtaatt taagattaca gagtatttga tatcgtgtat gcacgttata
     1801 gcatatatta agatatatat attcaatttt gagtgtatat aaatttaggt ggtaccacgg
     1861 aaaatatatc cgtcctattg atttaggacg gattttttaa tatagaaaat ttaggaggaa
     1921 aaatgaacgt aataggaatt ataggtgcaa tggatgaaga agttagtatt ttagttgatt
     1981 taatggatat tagagagact ataaaaaaag ctagcttaga attttataag ggaattttgg
     2041 aaggtaagaa tgtagtgtta gtaaagtgtg gcataggtaa ggttaactct gctctttgtg
     2101 cccagatact aataagcgaa tttaaggttg atgctattgt taatacaggt gttgcaggag
     2161 ctttaaacga aaaacttgat gttaatgata tagtaatatc aactgatgct atacagtatg
     2221 atgtagacac tacagcattt ggagacccta agggagttat accaagaatg aagacttctg
     2281 tatttaaagc agatgaaaga cttatagatg ctgcttacaa atcttctgtg gaagaagtaa
     2341 agactcataa agtattaaaa ggaagagtag ttacaggaga taaatttata aattctaaag
     2401 aattaaaaga agaattatta aatgattttg gtggatattg tggagaaatg gaaggtggag
     2461 ctatagctca tgtatgctat ttaaataata ctccatttgt aataataaga gcaatgtctg
     2521 ataaggcaga tggaagtgct gatgttactt atgatgtatt tgtacatgat gctgccaata
     2581 actctaaaga catagtttta aatatgttaa aatcaatata aatatataag gaggtacaaa
     2641 aaaatgagtg aaaaaaaagt tatattaagt ggtgcacagc catcaggcaa aatgacttta
     2701 ggaaattatt taggagctat aaaaaactgg actgaacttc aagacaatta tgattgttat
     2761 tatagtatag ttgatttgca tgcaataaca gtacctcaag accctaaagt tttaagagcc
     2821 aatactatag aattacttgc tcaatatata gcttgtggat tagacccaga aaaaaatact
     2881 atatttatac aatcacatgt caaagagcat gttgaactta tgtgggtatt gaatactatg
     2941 acttatatgg gtgaattaag tagaatgact caattcaaag ataaatcaca aaagagtgaa
     3001 gcaaatctaa atgcaggttt attcacatat ccagttctta tggctgctga catattgttg
     3061 tatcaaactg acctagttcc agttggagat gaccaaaaac aacatttaga attagctcgt
     3121 gatttagcaa atagatttaa taatagattt tcacctactt ttgtagttcc agaaggatac
     3181 tacccaaaag gtggagcaag agtgatgagt ttgcaagagc caactaaaaa aatgagtaaa
     3241 tccgattcaa atgaaaatgc atttatacta ttagcagatg attcagattc tataaagaga
     3301 aagataaaaa gaagtgttac tgattcttta ggtgttgtaa aatacaatga tgaacaacca
     3361 ggtattaaaa atctaattga tatatattct aatttaagta aaaagagtgt agaagagatt
     3421 gtaaacatgt atgaaggaaa gggttatggc atatttaaag aagatgtggc tgaagtagta
     3481 tcagaagctt taagacctat aagagaaaaa tatgttgacc ttcttaataa taaagattat
     3541 ttagaaaaaa tatatagtat gggagcagaa aaagccgaaa aacaagctag aaaaactctt
     3601 agaaaagttt ataaaaaagt tggatttata gagagaagat actaattatt agctatcttt
     3661 acttgataaa taaattttaa agttattaaa aaggtatatg atatgaaaga gagagtctca
     3721 aagtgaactt ttgttcatga ggcactcttt tttgtatgaa ataaaatata ttttaatcat
     3781 ttcaacaatg aaaaagagga ttatctctat tttgagacag catcttttga taataaaaaa
     3841 agatgagctt ttgctcatct caaaggggtt agattattct tactttgggg aaagtagaat
     3901 aaaggtaaaa gttaactgat gtcaactttg tcttttttgc atatcactac tgagcataaa
     3961 aaaatcagaa aaccatagcc ttctgaaaca acttctaaca tacattgtat tttataccca
     4021 atatgtaatt tatgagttaa ttttaactct tataatgaaa attgtcaata atatatcaaa
     4081 aaaatatttt ttaaatttat ataattaaat gaatttatgt ttatatatag gtgatatgat
     4141 acagataatt atctttttaa tacaatatag ttctaaaaaa gaatatatta gaagaagata
     4201 atatttaaat aaaaaatacc ttataaagtt aattttttaa tactttataa ggtaaataca
     4261 tattgtattg tggggataaa tttaaatcat ttgatactaa tcaacactaa gaaccaataa
     4321 ctctctatca tcttgagtaa ttgtatatat tcttaatttc ttgtatgtca taatattttc
     4381 tccacctgta aaataactcc taagattaac acaatcagtt ttaggttgat ttgtatctcc
     4441 taaataatta cgcaattctt tatctttagt ttcaatatct ttaaatccaa aatattgtaa
     4501 agtagcttca actctatatt tttggacata atcgagagct tctttattag tttatgttgg
     4561 tgtaaaatac ataccattta tataagcctt tattgtactt ggattaaatt taaaatctgc
     4621 agaatatatt ttatgtgtaa ctattatttc ttgaacagtt ggaattttaa cttctggttc
     4681 atcaagtatt tcaggagtac tatttagctc tgtaatagat aagttatcta agtatattgt
     4741 atcactatca attaatgcta tatttatatt agaagaatct ttttcagtag tttcaaattc
     4801 ataactaaat tttgtatatc cttgttctag taccaaataa tccgtttttt cttcttttac
     4861 ttttatattt acaattattg aattatatgt taaaggattt tttgaatttt actaaaaaca
     4921 taacgtttat aaggttttag ttcagacata ggtattttaa tcttcgtctc accatttaat
     4981 ctatttgctg agccttttaa accatcttga tttgtagtat taacattact ccatatacta
     5041 gggtaattat tgtaatcatc aaaattagta aagtaagttg gtgtttttat aagtatattc
     5101 attcctcttt caagtttaac attacatatt tgtttatcag acaaagtttt taaactgtct
     5161 ttaatcttat tgcctatatt atcatcaaat atgagttcaa cacaactttc atctattggt
     5221 atattattaa aatataacaa accatctttt ttagtagtgc caaaagtttt ttcaattgct
     5281 tctccaattg taagttcagg tgttttatct tctgggacct gtaaactttt agcagtaatt
     5341 cttcttttgt aagattcatt ttctgtatct aatataatag atacagaaat actgtcaatt
     5401 tgacctatat cgtcttacca actatttcat tctgttacta tttgtccaga accatttttt
     5461 gtaccaaaat ttccacttac ttgtgttgtt tctaatttaa tttgctttcc atcatctaat
     5521 ttttttaatt aattataatt tataggaatc agtctagaac taaattgata cattgtgtta
     5581 agagccatag gtgaaaatcc ttttttgaga taagtatttc caggagatag attattgcca
     5641 atttgatttt cttgtgcttt gatagttgat aatgcatctc catctaacac taaatttgtt
     5701 gttggtgtca ctttgtacat aggtgcagta cctgtgttgt agtatctaat atttgcattt
     5761 atatatgcag attctccttt gtttatactt aatccagtat ttcatgatta tccattgcta
     5821 ttttgaacag cagttgaatt atctgttgta tggtaataat ttgtcttgta tagtttcttt
     5881 aaactcatta aatttttcta atgaaatttc gttttgattt tttgttctta ttactttatt
     5941 aaatgcaaat ttttctggag attcaaaata atatacatac ataggtttat ctattttatt
     6001 ttttgatata gcatttcgaa gttctctata ttctttttct caagaatttg cttctatttg
     6061 ataatcataa aaataatttc ttgtctgaga atatttgctt atctctatag aatctttttt
     6121 atatgatcct aatacttctt tttcagattt ttcaagtttt tgatcttttc tttctcattc
     6181 tttagccttt tctttatctt taaaaaaatc cttctgatct ttctatagga gccttgtaag
     6241 tagtattaca aacttttcca ctatagcttt gtaaatcttg tgcataaatg ttattaggta
     6301 acaaactacc taaagttaga tataatatca acaatataga tatacaatta ctaatacttt
     6361 tatgtttcct aaattttttc atttattctc cctacaaata ttagtttagt taacttttga
     6421 attttaattt atttagatta tataatctaa ataagaattt ttt
//
LOCUS       DAGSWI010000179         6405 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.183, whole genome shotgun sequence.
ACCESSION   DAGSWI010000179 DAGSWI010000000
VERSION     DAGSWI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.183"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            419..1693
                     /locus_tag="MJF80_003546"
     CDS             419..1693
                     /locus_tag="MJF80_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBZ0282155.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKAFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGINPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1900..2217)
                     /gene="trxA"
                     /locus_tag="MJF80_003547"
     CDS             complement(1900..2217)
                     /gene="trxA"
                     /locus_tag="MJF80_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBZ0282156.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2484..3236
                     /locus_tag="MJF80_003548"
     CDS             2484..3236
                     /locus_tag="MJF80_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBZ0282157.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3223..4104
                     /locus_tag="MJF80_003549"
     CDS             3223..4104
                     /locus_tag="MJF80_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBZ0282158.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(4330..5685)
                     /locus_tag="MJF80_003550"
     CDS             complement(4330..5685)
                     /locus_tag="MJF80_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBZ0282159.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT         2448 a          933 c          816 g         2208 t
ORIGIN      
        1 acatctttta taacatataa aatctttttt tctaattata tacaatatat tttccattat
       61 gttctaatca atttctttac tatttttaca actttattag tcatattcat aattatttct
      121 aattagtgtt taacatttta tgtaaaattt ataaaaaaat gtatataaat taaaataagt
      181 gactatacta aaaatatgat acggataaac aataagttca acctatctcc cccaaaatca
      241 agtgagctac agtttccccc aactgtagct cattttcgtg taatttaata aacatctcaa
      301 caagaattaa ttaacaaaac cccttataaa aacataacat atattaaaca agctcttgat
      361 taaaacttga gcttttttaa taaaaaacag aatagtttat tagataaggg gtttttttat
      421 gcaattcatg gatgtatcta aatacattga aggtgcaaat cttaaagtta aaactaaaaa
      481 tggtgaaact gttaaaagaa tatattttaa caactctgcc actccattag ttttaaaaaa
      541 cgtagtggat aacttaaact gtgagattcc ttggcttact tatattaatg ctccaggaat
      601 aatctcagaa aaaaatactt taaaatatga aaatgtacgc tctactatcc tcaaacttat
      661 tgatggagac gaagaaaagg attctgttat ttatgtcaaa aatgctacag aaggaataaa
      721 cttcttagca aaatttttta aagatgaaaa tccagataaa tttgttatta ccacagcaat
      781 ggagcatatg gctaattacc tgccttttaa agttaatttt cctacaaaag ttgttggaat
      841 tacagaaaaa ggagaacttg atttatgtca attagagaat atacttaaaa attatgctgg
      901 taatgtatct ctcgttacag ttacaggagc tagtaatgtc acaggtataa ccactcctat
      961 atatgaaata gcaagaatag ctcataagta tggtgcaaag atattggtgg atattgtgca
     1021 agtaattcag cataaagctt tttcaatgat gccacatgaa tgtgatgagc atatagattt
     1081 tgttgttttt agtgctcata aatgttattc tccactaaat ggaggagctc tggtaggacc
     1141 taaaaaattc tttgaaaaat ttaatcctgt actatatggc tcaggttcta ccaaatttgt
     1201 aaatgataac aaaatactat atgcaaacgt accccaacgt tttgaagcag gatacccaga
     1261 gtattttgga actcttgcaa tgggaaaagc actgaacact ttaaataaaa ttggtctaaa
     1321 cagaatttca aaatacgaaa gagaattatt tttatatgct aaagaagaaa tgagtaaaat
     1381 acctaatata ataatatatg gagataaatc aaataatgta attatcccaa atatatcttt
     1441 taatataagt aatatgtatt acaaagatgt agcaaaatat ttagttgata actttggaat
     1501 agaaactgga gctggagtag taggtgcaga tatttatgta caaaaattat taggtataaa
     1561 tccaaaagag gcatacgtca gatttatgag ggaaaatcct gtaggtcttg ttaggataag
     1621 cttaggaatg tacaatactt ttgatgaggt ggatagcttt atacatgcta ttaaaacttt
     1681 ggcttttaaa taagattttt ctattctttt ttatcaataa atagagagta gttttgtttt
     1741 cacaaaaaaa caaattatat caacagtcaa tttcattagc aattagacac tcatttgtaa
     1801 ctatacaaac tattttacac tatacaaaca aaaaactcat tacaatgagt ttttttgttt
     1861 atatatgaga tttattttaa ttacttcaat ttactttaat tacatatatg attcaatttt
     1921 gttttttaaa ttctccttag gcataaaacc tattaatcta tctacaggtt ttccatcctt
     1981 aaatattagc atagttggta ctgtagatac ttcaaatttc tgagctaatt ctatactttg
     2041 gtctatgtcc accttcaaga agtttgcttt ttcaaccatc tcatttccta aagcttcata
     2101 tattggtgat agcatcttac aaggtccaca ccaagtcgca aagaaatcta caactactaa
     2161 accatcttta ttttctactt catttataaa ttcattatta tttattattt ttaccataat
     2221 atacatctcc tttattatct tcttgatatt aatatactac gccctatata acagttcaat
     2281 taggcacttt tttgtaaccg cctattttta atagatttaa tattatgata aatagattta
     2341 taagattaaa tatttatgag actaaatatt tctaatatat tttatttttt tcttaatata
     2401 gttgacattt tttcccttaa attataaaat caaattaata actacatagg tagttactaa
     2461 tcttttatca aaggagtttc tatatggata atataattaa tgaattacaa aatataggat
     2521 ttactaaata cgaatctcaa atatacatat cattattaaa ggaaagccct ttaacaggat
     2581 atgaaataag taaattatca ggtgtaccac aatcaaaagt ctatgaaaat ataacaaagc
     2641 tattaaacaa taatataatt atcaatgttg gaactgaccc tattaagtac ataccaataa
     2701 accctaatga attattaaaa aataagaaaa aagaatttga caattctatg gataatctaa
     2761 agaatagctt acagatatta aataaaagta aaagtgtaga atacgtatta aacataaaag
     2821 gagataaaaa tatattaaaa aaagcaatag aaatgataaa aaatgcaaaa caagaaatta
     2881 taatttcttg cccttatgaa gaaattcttg aattaaaaaa agaactccta attgcttaca
     2941 aaaaaaatat taatattgaa atactacttt tagaaaacaa aagtctgaaa ggtcttgata
     3001 atattcatat tcatggaggt aataaaacta cacttgaaaa taaaatgctt tacaaaggaa
     3061 ttattttgat tgtagataat gatgaaatat tgacaggaaa cttatcagaa ggcagtgagg
     3121 ccatttcaat ttggacaaaa aactctaaca ttttatatat tggagttcaa tacataaaac
     3181 atgaaattta tatttcagaa caaataaaag gaggttataa aaatgaacaa aattaagatt
     3241 aaacaagttg atgcttttac aactatacct tttggtggaa atcctgctgg tgtcgttact
     3301 gatgcctcta acatttctga tgaaattaag cagaatgtgg ctaaagaaat gaatttatca
     3361 gaaactgcat ttgtttcaga gtcaaatgtt gcagatttta aggtacaatt tttcacacca
     3421 ggtttagaag ttgatttatg tggtcatgca acaataggta ctttccatgc acttttggaa
     3481 gaagggaagc ttaatactaa taagagtata ttttatcaag aaacaaaagc tggtgtatta
     3541 tctgtagagc ttaaggaaat aaataatgaa aatatattta tgatggaaca gaatacacct
     3601 gaatttgaaa atttagataa ttgcagaaaa gaacttgcac aaatgatagg tgtgcatgaa
     3661 gatagacttc ttgaatatcc tattataaag gtaaatactg gactttggtg gcttgtattt
     3721 ggtctaaaat ctttagatga tttaaaaaat atctctccag atttaaataa aatcaaaaaa
     3781 tttagtggtg ataataatat aattggtata actccatttt gtatagaaac tatagataaa
     3841 aagtgtgact accatttaag atctatagca ccatatgttg gagttacaga agaccctgta
     3901 tgtggaactg ggaacgcttg cgtatcatct tatatagtac atcacaatat tttagataaa
     3961 actagtttta ttggtgagca aggtaacttt ataaacagac ctggaaaggt atatgtaaat
     4021 ataactagag aaaacaatga cataacttct gtaaaaattg gaggaagtgc ttatactgtt
     4081 ttaagtggtg aaattatgta ttaatcccat acctaataaa aacaaataaa aatactagct
     4141 aactaaagct agtattttta tttgttattt ttatttaata atcatactct aacaaatatt
     4201 ataattatat atatcataat attgtattta gaagtaaaat tttattttta ttattaatta
     4261 gttataaatc aatctaatac aacataatta cagtagatta acctataaaa cataataatt
     4321 gcttactgat tatttatcgt cctcaacaag tctacttaat gtcttatcag ataagaataa
     4381 taatatcgct gcaacaaata gtattactgg aattatagta tatactttaa tcatatcaaa
     4441 tttaagagta aatgcatata tatatccgta tcctttacca gctataaaag tagcaaatgt
     4501 ccatactccc ataagaacac ctagaagctt cttaggtgca tacttactta caaaagagtt
     4561 accaagtggt gagaataaca tttcaccaag acttaaaaga ataccaaaca tgattatcca
     4621 tagtatacta gctttagcag tttcaggagc tccaacccct cttgaaaatt ctgcaccaac
     4681 aagcattaag aatgcaagac ctaagaatat taatccaaga cctgtctttt taaataaact
     4741 catatctcct tgtggtctac tagcaagttt aaaccataat gctccaagaa ctggtcctaa
     4801 tactatacat acaagtgaat ttaatgagtc aaaccaagca agaggaacat caaatcctcc
     4861 aacattcatg tttacaaatg ctgcaccata atcataaact gcaagataag ttagatacca
     4921 gaatacccag aaaattacag agaagaatga tataagtata atagcccaaa ttctcttctt
     4981 atccatgctt gtaagaggag acttatcttt ttcttcaatt ttttcagcaa ctacaccttc
     5041 tttaaaaggt ctctttcctg cattcccaag gaatctccat ccatatatga accatattgc
     5101 acctattaca caaagtacag ctgctataaa gaaacattga gaaaatccaa gtacaccatt
     5161 ttttgcaaat gttttaaggt aaagaatacc aactgctata gtaccaataa aagaacctat
     5221 attaacaaaa gaatattgta ctgaaaatgc tgtatcaagt tcttcttgac tatcaaaaag
     5281 ctgtccattt accgcagata cgttaccttt aaagaatgca gtaccaatcg aaactaagat
     5341 tatcattgca tttaccatag aaactgtagt tgcaatagaa ccaagatagt atcctacccc
     5401 catgataagc atacctatag gtatagtata tctagctcct atccatctat ctgatatata
     5461 tcccccaatt ataggtgcaa ggtatgtgaa tgcaacaagg ttggcttgca ttatagcagc
     5521 ttgtccttta tctattccaa gaccaccatg aactatagct gttgtaagga atatatatat
     5581 taagtacttt gaagcataat atgctgctct ttcaaatgag aaagttgttg agcagatata
     5641 aaatcccata ggaaatttac ccttcttttt tactgttgag ctcataattt taaatccttt
     5701 cttaattgta tttttatttt ttacgttttg tccaaattct ttcttttatg aaagatataa
     5761 tattattatt ttgtaaaaca atttgtaatt attattaaga taattgcgca tttatttgta
     5821 gagtacatat tctcatggaa taaaattaat acacttctta atcataaatt taaattatat
     5881 aaattcacta ttttcagata tagttataaa ttaatatatt ctcatctaat accatgaaaa
     5941 aaccttcgcc atatgcaatt cttatttttt acgtgatagt attttacaaa ctaaaatccc
     6001 ccttttgtag caacttctgt aattacattt tagtgctttt ttaaaggagt ctcgaataaa
     6061 gcaacatata ttttgttcaa ataatgtttt tatttacttt ttaaatttat atattacttc
     6121 acccaaccaa ttctcaactt aaataaataa actactatct attcacagat tagccctatt
     6181 tcttacacat tagtttataa taaacactgt gaatctaaac ataaaaactg ttattttaaa
     6241 gtaattaccc atatattcgc tttgatacta tttattgagg ttatactctt tatgtataat
     6301 atattattta taaaatacct caaaatatat tattcataaa atacctcaaa ctgaaaactt
     6361 tttcacgaat atttattttt cacctcccct tttttatttc accat
//
LOCUS       DAGSWI010000180         6355 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.184, whole genome shotgun sequence.
ACCESSION   DAGSWI010000180 DAGSWI010000000
VERSION     DAGSWI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.184"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            188..1132
                     /locus_tag="MJF80_003551"
     CDS             188..1132
                     /locus_tag="MJF80_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBZ0282160.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1154..1780
                     /locus_tag="MJF80_003552"
     CDS             1154..1780
                     /locus_tag="MJF80_003552"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBZ0282161.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1814..2863
                     /gene="hisC"
                     /locus_tag="MJF80_003553"
     CDS             1814..2863
                     /gene="hisC"
                     /locus_tag="MJF80_003553"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBZ0282162.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2866..3444
                     /gene="hisB"
                     /locus_tag="MJF80_003554"
     CDS             2866..3444
                     /gene="hisB"
                     /locus_tag="MJF80_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBZ0282163.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3446..4051
                     /gene="hisH"
                     /locus_tag="MJF80_003555"
     CDS             3446..4051
                     /gene="hisH"
                     /locus_tag="MJF80_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBZ0282164.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4048..4770
                     /gene="hisA"
                     /locus_tag="MJF80_003556"
     CDS             4048..4770
                     /gene="hisA"
                     /locus_tag="MJF80_003556"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBZ0282165.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4752..5510
                     /gene="hisF"
                     /locus_tag="MJF80_003557"
     CDS             4752..5510
                     /gene="hisF"
                     /locus_tag="MJF80_003557"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBZ0282166.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5538..6206
                     /locus_tag="MJF80_003558"
     CDS             5538..6206
                     /locus_tag="MJF80_003558"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBZ0282167.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2590 a          533 c         1253 g         1979 t
ORIGIN      
        1 taaagggatt gtaatatgaa attaattcat agacacaagc cctgttttta gtgtaattgg
       61 tcttgtatct atgatgggaa gtaattttaa gagaaaatta tagccatata ggtataaagc
      121 ctatatggct tttttattgt tttaaatttg caaatataag agtaaattta atgaagaggg
      181 tgagaatgtg aaatttttga ggattgaaga tgaggttatt tattctaaaa aaagatataa
      241 attaaaaagg gaaatagata aacttttttt ggatgaagaa tattttcaaa tagaaccaca
      301 attatttgaa gaatatgatg aatttacaac tataaataat aaaattccag aagaatctat
      361 ggtaaaggtt gtaaatggaa aagtaatggt acttagagct gatataacta caagtataat
      421 aaaaagcctt gtaccaagat gggaagatgg tttaaagctt aagctttttt ataattcatc
      481 aatatataaa aataaaaata cagttggaat aaaagagata agacaaatag gttgtgagta
      541 tctaggagaa gcctctgtag aagcagatag agaagtagtg aaattagctc ttaagatact
      601 tgaaaaatac aataataatt ttattctaga ggttgggagt agcaagtata ttcatggttt
      661 gttagaagaa ttaaatttaa ataagaattg tgaaaatcaa attaaaaatt tactatatac
      721 gaagaacact catgaattaa aagtatatat tgaagaatta aaaattaagc gtgaagtaaa
      781 agagttatta tcaaatattt taagtttaca agggaattta tataatgtta ttgaaaaatc
      841 aaacaagttc tattgtaata ataagatgaa gcaagctctt gaagaattaa agcaagtaaa
      901 taatttgatt gaggaatgta attttttaga taaggctaga tttgatttat caatgattac
      961 tatgcttgac tattatgaag gaattatgtt tagaggatac tacccaaact catataaaga
     1021 aattcttagt ggtggaagat atgattcttt aactaaagag tatggaaaag aaattccagc
     1081 cattggattt acactaagtg ttgatgagtt aatgaagtat gtatataagt aaagtttaga
     1141 aggagggatt gctttggatt atttgactat agcactagca aaaggacgaa ttgaagggga
     1201 atcatttaaa aaatttaaaa aaatgggtct aggagatagt atagacacag atacgagaaa
     1261 actaattttt aaagatgaag aaaataaaat tatatatata catgtaaagc catcagatgt
     1321 agttacatat gttgaaaaag gagtcgctga cctcggtata gcaggtaaag atactatttt
     1381 agagaatgag acagatgtat atgaaatata tgatttaggt tttggaaaat gtaaatttgc
     1441 tgtagcagga ttaaagggag atagcatata tagagaagat gaatatttaa aagtggcaac
     1501 aaagtatccc aatatagcaa aaaagtattt taaagagaaa ggtcaaaaaa ttgaaataat
     1561 aaaattaaat ggttctgtag aactagctcc aatagtaggg ttgtcagatg taattgtaga
     1621 tatagttgag actggcaata cacttaaagc aaatggtctt gaaatattag aagacatatg
     1681 taatataagt gctagaatta tatcaaatag agctagctat agatttaaat atgaacaaat
     1741 tcagaatata attcgtttat ttgaagaact tgataattaa aatataattg agctttataa
     1801 ggagggattt aagttgagag aaaaagaaag cataagagag ttaaggggat atgaaccaaa
     1861 tcacgtaaac tgtaaagtaa aacttgatgc aaatgaagga agtaagaggt tgtttaaata
     1921 ccttattaaa gaaatttcag atagtgatat agatttaaat ttatatcctg aagattctta
     1981 tagtgatttg aaagaatcta taatagatta cataaatatt tctggagtaa ataagaaaaa
     2041 tcttctagta ggaaacggtt caagtgaaat aattgattta ataatacata cttttgtaga
     2101 taaagatgaa gtaatattga gtttctctcc aagttttagt atgtattcta tatatagtca
     2161 gataaatggg tctaaattta taggtgttga aagtgatgaa aatttagtta taaatataga
     2221 taatgtgatt gaaaaagtaa aggagaataa tcctaaaata gtaattgtat gtaaccctaa
     2281 taatccaact ggaacaatat taaagaggga agagataata aagcttttag attcaactaa
     2341 tagtctagtg gttttagatg aagcatatat ggattttgga gaagaaagca tgttaagtga
     2401 tgtttttaag tatgataatc taatagttct tagaacttta tcaaaggcat ttggattggc
     2461 tggaataaga acaggatata tgttatctaa tagtagttta ataaattctg ttgagaaagt
     2521 aagacctcca tataatttaa attcattatc agactttatt gctacaagag cacttagaaa
     2581 taaagatgtg gtaaaggctt atataaagga agtaaaagag gaaagagaag ttttatatga
     2641 agaaatgatt ggtatgggaa ttaaagcata caaatcacaa gcaaatttta tattatttta
     2701 ttctgaaatc gaaaatttat cacaaaaact tattgataga ggagttttaa taagaaaatt
     2761 tggtggaaag ttagaaaatt attacagagt tacaattggt gataaagaag aaaattctat
     2821 gtttgttgga gctataagag atattttgaa aaaggagaaa taggtatgag aatttggaag
     2881 gtagaaagaa atactcttga aacacagatt ttagtagaat taaatattga tggttctggt
     2941 aaagctgaaa tagatactgg aataggattt ttagaccata tgcttacctt aatgtcattt
     3001 catggcaagt tcgatttaaa agtgatttgt aagggtgata catatgtaga tgaccaccat
     3061 agtgtagaag atataggtat agctataggt gaagcattta aaaatgctct aggtgacaaa
     3121 aaaggaatac gaagatattc taatatctat atacctatgg atgaatctct atcaatggta
     3181 gcaatagata taagcaatag accttatctt gtatttaatg ctaaatttga tacccaaatg
     3241 attggtagta tgagtactca atgctttaaa gaatttttta gagcttttgt aaatgaatcg
     3301 agagtaacat tacatatcaa tctcttatat ggggaaaatg accatcataa gatagaaagt
     3361 atttttaaag catttgcaag agctttaaaa gagggtagtg aaatagtttc taatgagata
     3421 gcatcttcaa agggggtttt gtagaatgaa tataatagta gactatggtc ttggaaatat
     3481 agattctgtg tctaggggtt ttagaaaagc tggaatagaa actaaaattt ctagtgatat
     3541 agatgaaata aaacaggcag attcacttat acttccaggt gttggagctt ttagagattc
     3601 tataagtgcc ttagataaac taggattgat accaataata aaggagcatg tttctaaggg
     3661 taaatttatg ataggtatat gtctaggaat gcaattactt tatgaaaaaa gttatgagta
     3721 tggagaatat gaaggattag gacttataaa aggaagtatt gataaactgg atataagttt
     3781 aaaagtgcca catatgggat ggaataatct aaaatttaat aaggcaaatg atgatatact
     3841 taaatatata aatgaagatg attatgtata cttcgttcac tcatattatg caaattcatc
     3901 aaatgaagaa ctaattgcat tttcagaata tgaaaagaaa atccctgcta ttgttagaaa
     3961 aggcaatgta tatggtatac aatttcatcc tgaaaaaagt ggagaagtag ggttaaatat
     4021 attaagagca tatggggaga tgataaaatg ataatatttc cagcaataga tataaaagat
     4081 aataagtgtg taagactcac acaaggagaa tttgacaaag taaatgtata ttatgataat
     4141 ccattagaag tagcatataa atggaaaaat gaaggagcag aatatataca tatagtagat
     4201 ttaaatggtg ctagaagtga atttggtgta aatacaaaaa taattgagga catagcaaac
     4261 aatatagata tacctattca agttggcggt ggagttaggg ataaggaaaa agtcaaaagt
     4321 ttaataaatg ctggtgttac tagagtaata ttaggcagta tagcgattga aaatttgaat
     4381 ttagttgaag aattggtcaa tgaatataaa gagaaaattg tagtttctat agatgctaaa
     4441 gatggaaaag tagcagttag gggatgggaa gttgtaagca atgtagactc attaacatta
     4501 tgtaaacaac ttgaaaagat aggtgtacaa actattgttt atacagatat atcaaaagat
     4561 gggatgttgc aaggtccaaa ttttgacata tatgaaagaa tagcaaagga aacatcacta
     4621 aatgttattg cttctggagg cgtaacttct atagaagacg taaaaagact aaaagctatg
     4681 aacttatatg gtgcaataat aggtaaagcg ctttatgata aaaagataga ctttaaggag
     4741 gcacaacaat tatgcttact aggagaataa taccttgtct agatgtgaga aatggaagag
     4801 ttgtaaaggg gaaaaaattt aaagatatag tagatgttga tagtccagaa gtacttggaa
     4861 aattttatag tgattgtggt gcagatgaac ttgtattcta tgatataact gcatcaaatg
     4921 aagagagaaa gacatcttta gaatttgtga caaaagttgc agaaaatata aatataccat
     4981 tttgtgttgg tggaggagta aataaattag aagatttcac tgatatttta agaaaaggtg
     5041 ctgataaagt ttctataaat tcatcagcag tcaaaaatcc tgaattaata agggaagctt
     5101 cattaaaatt tggagcacaa tgtgttgttt tatcgataga tgcaaagaaa aatgaagagg
     5161 gttcatggag cgtatacgta aaaggtggaa gagaaaaaac taatctggat gcaattgaat
     5221 gggctgttaa aggtgtagaa cttggagcag gagaaatcgt tgtaaatagt atggatgaag
     5281 atggaatgaa aaatggatat gatatagaat tattatcaaa gataacttct ttagtaaatg
     5341 tacctgttat agcatctgga ggcgctggaa aaaaggaaga tttttatgag gcggttaata
     5401 aatccaatgt agatggaatt ttagcagcat ctgtttttca ttttggcgag ataaaaataa
     5461 acgatttaaa gaaatattta aaagacatgg gtgtagaagt aagattataa gttaaatact
     5521 aaaaggagat tagaaatatg gataataagt gtaacaatgt atacagtgat gaagtagaga
     5581 aatttataag aagtataaag tttgatgata aagggttagt ccctgttgtt gttcaagagg
     5641 tagttagcaa agatgtactg atgttagcat atatgaataa agatgctata aagaaaacct
     5701 taaaagataa agtagcttgc tattttagta gaagtagaca agaactttgg gttaaaggtg
     5761 agacatctgg taatactcaa aaagtagtta agatgtctta tgattgtgat gtagatacta
     5821 tactactttt tgttgaacaa actggtgtag cttgtcatac aggcaattat tcttgttttt
     5881 acagagactt gtttgatgat agttcaaaaa tggagcttga agtacaaaca aatatattga
     5941 aagaattata tgatttaatc aatgaaagaa aaaataatcc agttgaaggt tcttatacta
     6001 attatctttt tgaaaaagga atagataaga ttcttaaaaa agtaggtgaa gagagtagtg
     6061 aggtaattat agcatcaaaa aataccgata aaagtgaact tatttacgaa attagcgatt
     6121 tagtatatca tacattggtt ttaatgatag aaaaaggtgt tgaaattgat gaaataaaaa
     6181 aagaattact taaaagaaga aaatagaata agatagatta atgggtaata agaaaatata
     6241 ccgcttttaa taaaaagtgg tatatttttt attttatatt ttgatttata gaataatatt
     6301 taaattactt gataaaatta actatatata taatttattg ataaaataat tctag
//
LOCUS       DAGSWI010000181         6340 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.185, whole genome shotgun sequence.
ACCESSION   DAGSWI010000181 DAGSWI010000000
VERSION     DAGSWI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.185"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(107..622)
                     /locus_tag="MJF80_003559"
     CDS             complement(107..622)
                     /locus_tag="MJF80_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBZ0282168.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(985..2055)
                     /gene="leuB"
                     /locus_tag="MJF80_003560"
     CDS             complement(985..2055)
                     /gene="leuB"
                     /locus_tag="MJF80_003560"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBZ0282169.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(2062..2553)
                     /gene="leuD"
                     /locus_tag="MJF80_003561"
     CDS             complement(2062..2553)
                     /gene="leuD"
                     /locus_tag="MJF80_003561"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBZ0282170.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2568..3845)
                     /gene="leuC"
                     /locus_tag="MJF80_003562"
     CDS             complement(2568..3845)
                     /gene="leuC"
                     /locus_tag="MJF80_003562"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBZ0282171.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3802..5544)
                     /gene="leuA"
                     /locus_tag="MJF80_003563"
     CDS             complement(3802..5544)
                     /gene="leuA"
                     /locus_tag="MJF80_003563"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBZ0282172.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5668..5914)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6062..>6340)
                     /locus_tag="MJF80_003564"
     CDS             complement(6062..>6340)
                     /locus_tag="MJF80_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282173.1"
                     /translation="QGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKI
                     IEKIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
BASE COUNT         1914 a         1271 c          649 g         2506 t
ORIGIN      
        1 ttttagtata actaatttat ttttatcttg tatttttatt gtatttttga attttatttt
       61 ttctaaatat aaaatttatg ttttaaaata taaaattttt atttaatcaa agttcaatat
      121 attccacttt ttctttcgat tcttcaaaaa accattcttc attagaaaaa tcaccttctg
      181 caatttgtcc aataggcatt ttcatatact ttccagttgc tgtcactgct atatctccat
      241 ttggtagaat tatttctcct gtaccttcaa ataattttct gttttcttta gttattcttc
      301 caacaacttt tatagtagca tctgttggta ctggtttctt atatttcaac tctaaagata
      361 ctgttactcc ccatatttgg tcatcattta tacttacagc tcttccaata gtctcatcca
      421 atattgcagc agacatacct ccatgaactc tcccaggata actttgatgc caatcctttg
      481 tattacaaat tgatacaagc tctccatttt caagttcata aaaatctgct ttaagtccta
      541 gctcattttg tgttccacaa actaaacaag atttactact attttgcttc ttcaaaactt
      601 tatactttgc caaaaaatcc atctcctaac cgacattagt agtcgcttaa tattaaataa
      661 ttctaaaata tattatataa tattaataaa atccctgcaa acttctaata ataaaaatag
      721 gctacttcaa tatgataagt ttaatttaaa gtattatttt ccaaaaaata actttatcaa
      781 aatagaaact caaagtagat ataaacctct ttttcgttat taaaatagta aaaatatatt
      841 tgacttaata taaaaaagag tgtctcatta actaataaag tttattttga gacacccctc
      901 ataaaacaaa ttatactaca atagattttt actaattcta caagttatat atttaaatta
      961 tattattcaa attaaactat aattctaaat ttcatttact attaattcgc ccatttccct
     1021 tgttcctaca acatttccca caccattata gatatcaata gttctataac ctttattgag
     1081 tacactttgt acagcatctt caatacactt agcttcttct tctaagttaa aactatgtct
     1141 taacatcatg cttacagata gaatagtcgc aattggattt acaatatctt ttccagctat
     1201 atcaggtgca cttccatgta ttggttcata cataccaaaa ctatcttctc taagtgatgc
     1261 tgatggtaac atacctatag aacccgtaat catagatgct tcatcagata ttatatctcc
     1321 aaatatatta ttagtcacta ttacatcaaa ttggctaggg tcttttacaa cttgcatagc
     1381 agcattatca acatacatat gtgacagttc tacatcttca aactctttag ctaagtcttc
     1441 cattatactt ctccaaagtc ttgaactctc aagcacattt gccttatcta cacttataag
     1501 ttttttattt ctatttctgg ctatttcaaa agctctctta ccaattcttc ttatttcatt
     1561 ttcgttatat atttctacat cataagcatt ttcgatacca tcaatgattt ttcttccacg
     1621 ttctccaaaa taaatgcccc ctgttaattc ccttacaaca acaaaatcaa tgcctttctc
     1681 aactatatcc gttctaagtg gtgaagcttc ttttatactt tcatacattg tagctggtct
     1741 taaatttgca aataaaccca atgactctct taattttaaa agaccagact ctggtctttt
     1801 atctccctta catttatccc actttggtcc tccaacagca ccaagcaata cagcttcgtt
     1861 ttttcgacaa gtttcaacag ttttattagg tagaggttct ccagtttcat caatcgcgca
     1921 tcctccagct aatacatatt cacaatcgaa tctatgatta aatttacaac aaattttatt
     1981 tagaaccttt ataccttcat ctataatttc cggtccaaca ccatcacctt ttatgactgc
     2041 tatattacaa ttcatacttc actaccccct atttgctttt attgagttaa ttaagccttc
     2101 atttaatata atattttgca taaactcagg aaatggttct gctttatatt cttttccttt
     2161 tgttatattt tttatcacac ctgtagaaaa atctacttct atattatccc cttcatctat
     2221 gttattggca gcttcttcac attcaagtat aggaagccct atatttatag agtttctaaa
     2281 aaatattcta gcaaaagtag aagcaatcac acaggatact ccactttctt ttattacaat
     2341 tggtgcatgt tctcttgatg aaccacaacc aaagttttta gttgcaacta taatatctcc
     2401 tttttttacc ttagaaataa atttatcatc aatatcctcc atacaatgcg ttgctagttc
     2461 tttataatca gctatattta aatatctagc tggtattatt acatctgtat ctatattgtc
     2521 accgaattta aaaacacttc cattggcaat cataaatttt acctccctta aattatttct
     2581 gatggttttg atatttttcc agttatagct gatgctgctg ctacagctgg gcttgcaaga
     2641 tacacctctg aatcaacatg ccccattctt cctacgaaat tcctatttgt tgttgaaatg
     2701 gatttttctc ctgctgcaag tatacccata tgtcccccta gacatggacc acaagttggt
     2761 gtagaaactg ctgctcctgc ctctataaaa gtggtaatat accctttttc tatagcttca
     2821 agatatactt tttgagttcc aggaaatatc ataactctaa ctccatctgc tacttttttg
     2881 cctttcatta tctcagctac tacttcaagg tctgatattc ttccatttgt acaagagcct
     2941 attacaactt ggtctatctt tatatcctta tctatctcat ctactgttct agtattttca
     3001 ggaagatgag gaaaggctac tgtttctttt agttcagcta aatctatttc gtatacttca
     3061 tcatagactg catcttcatc tgcttcatat atatttacat cttttgtcat agttgaacac
     3121 ttatgtgaat ttatatattc catagtctta tcgtctactg gaaaaattcc attttttgct
     3181 cctgcttcta ttgccatatt acaaatagta aatctatcat ccatagataa gtattctact
     3241 ccatctccac aaaattccat tgacttgtaa agtgctccat caacacctat ctcccctatt
     3301 atatgaagta ttatatcttt accacttatc catttatttg gtttattctt caaaacaaat
     3361 tttattgctc caggaacctt aaaccaacat tttcctgtag ccatacctgc tcccatatca
     3421 gttgaaccta caccagtaga aaatgctcct aatgctccat atgtgcaagt atgagagtca
     3481 gcacctataa ttacatctcc agcagttact ataccttttt caggaagaag aacatgctct
     3541 attcccatat ctcctacatc aaaaaaattt acaactcctt ttgattttgc gaaacttctt
     3601 gtaaatttac attgttctgc actctttata tctttattag gtgtaaagtg gtctaaaacc
     3661 atcactactt tagtcttatc ataaacatct tcaattccta atttttcaaa ttctctaatg
     3721 gctactggtg atgttatatc attacctaaa actacatcta aatctgcttc gattaattgc
     3781 cctttcttaa cttcatttaa tttagcatgt ttggctaata ttttctgcgt catagtcatc
     3841 cccatttttt atttcctcct caatataact tttatttatt gcattcataa gtgcattaat
     3901 agctgctgta tttatgtttt ccgatatact tacaccaaaa tagacttcat tattgtgctc
     3961 tattcctaca taagctaccc ctcttgaatg tgacccttcc tctaatgcat gttcccagta
     4021 atatctaaat ttataatctt tcatgccaca ttgctttagt gcattattaa atgaatctac
     4081 aggtccattt cctataccaa caatcctttg ttcatttccc atataactta ttcttgcagt
     4141 catctcaatc atgttagtat cattagaatc atcattttca tagtttaatt catccattga
     4201 ttttatttta tacttcttaa ctttataagg gctctcaact tcaatatatt ctttcttaaa
     4261 caaattaaat attgcttcac tactaagttc ttccccaatt ctatcagatt ccattttgac
     4321 tacatttcca aaatcagaat gcatattctt aggtatcata aatccataat ctgtttccat
     4381 tatataaaca gctcctcctt ttccagattg gctatttatc cttataattt ctttgtattc
     4441 tcttccaata tcatgagggt ctattggtaa atatggaact tcccagtaat cattatctct
     4501 gtttttcatt gctatcatgc cttttctaat agcatcttga tgtgaacctg aaaaagcaca
     4561 atgtacaaga ttacctgcat atggatgtct atcatgtacc ataagttttg tacagtcttc
     4621 atatatacct attatcttat ctatattgct aaaatctagc tctgggtcta ttccttgtgt
     4681 ataaagatta agtcccacat ttactatatc catattccca gttctttcac catttccaaa
     4741 tagtgttcct tcaagtctat cggcaccagc caataatcca agttcacttg cagccgtaca
     4801 agttcctcta tcattgtgag tatgcaaact tagtataata gaatccctac aacttatatt
     4861 tttacaaaac cattctattt ggtctgcata tacgtttggt gttgccattt caactgttga
     4921 tggaagattt attattactt ttttttgagg agttggtttc caaacatcta tcactgcctc
     4981 acatatttca agtgcataat ccatctctgt tcctgtaaaa ctctctggtg aatattcaaa
     5041 agtaaattct gtttctggat atttttttac ttcttcatta aataattttg ctccttcaac
     5101 tgctatatct attatttctt gtttactcat attaaaaacc acatctcttt gaagaacaga
     5161 tgtagagtta tataaatgta taattgcttt tttacatccc cttattgatt caaatgtttt
     5221 ttctataaga tgagccctag attgtgtaag tacttgtata gttacatcat ctggaatcat
     5281 attctcatct attaattttc ttaaaaatgt atactctgta tttgatgctg atggaaatcc
     5341 tacctctatt tctttaaatc ctgtatcaat aagcattttg aacattctga ctttttcatt
     5401 tacactcata ggtgttggca gtgactgatt tccgtctctt aaatcgacac tacaccaaat
     5461 tggagcctta aatatttcat tatctggcca tttacgattt actatttgca ttttatcata
     5521 ttttttatac tttccacact tcatgttatt tccccctttt tttgtttttt gtattcagta
     5581 tacctcatat cgacgcaatc ctgcacaatt cataaggata aatgtcaatt ttatcattag
     5641 aataaaaaaa acctcatctc taataaaatt agagacgagg ttttgctcgc ggtaccactc
     5701 tatttagctt tctaaaaagc tcccttagta atagccaata caataattat aatgactata
     5761 tttcctataa cgtggaatta ctccgattat tctaagattt aaatcttaga aaacagctcc
     5821 tagacaagtt cagaactatc aactactaaa tcacaccatc ctttagctct ctgaaagttt
     5881 cattgttctt actacttcta ttcatcactt ttttaatcat ttaaattttc taaatatttt
     5941 gatttaatag ttattatata cacattaaaa tgtattgtca acacaaaaaa taattatctc
     6001 aattttttat ttttgataag caattctata aaacatgcta actattgaaa acagtatgat
     6061 attatgcctt aaactttttc atcttttcat ctatgatatt caataatttt ttttcatctt
     6121 ccaaactcaa tgcccttaat ctaattttat aattagattc gtcagtgtat atatttaata
     6181 tattttttac tacttctatt ttttctatta tcttataatt aaacttttga ctttgtttag
     6241 attttatact gaatatacat tgattattta ttaatattga atgattatcg agagtaatat
     6301 atacttttcc ctcatattta tttgaaaaat cacgaccttg
//
LOCUS       DAGSWI010000182         6291 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.186, whole genome shotgun sequence.
ACCESSION   DAGSWI010000182 DAGSWI010000000
VERSION     DAGSWI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.186"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(313..720)
                     /locus_tag="MJF80_003565"
     CDS             complement(313..720)
                     /locus_tag="MJF80_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282174.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1194..2078)
                     /locus_tag="MJF80_003566"
     CDS             complement(1194..2078)
                     /locus_tag="MJF80_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBZ0282175.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2369..2845)
                     /locus_tag="MJF80_003567"
     CDS             complement(2369..2845)
                     /locus_tag="MJF80_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBZ0282176.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQAKDLEEHHHHDENCGCGCNHHHEN"
     gene            3112..4287
                     /locus_tag="MJF80_003568"
     CDS             3112..4287
                     /locus_tag="MJF80_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBZ0282177.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4368..5171)
                     /gene="proC"
                     /locus_tag="MJF80_003569"
     CDS             complement(4368..5171)
                     /gene="proC"
                     /locus_tag="MJF80_003569"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBZ0282178.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5211..5795)
                     /locus_tag="MJF80_003570"
     CDS             complement(5211..5795)
                     /locus_tag="MJF80_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0282179.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2074 a         1065 c          712 g         2440 t
ORIGIN      
        1 taccaatagc ttgtagtgta cttccctcaa gaggaatagt cgttgaagtt tctcctattc
       61 ggagtttacc tgctgattgc ccattttaaa gtacttagga tttaacctta taccatctaa
      121 ccaatttttt ctactttcgt aactttcaca cttaggttta tttcatcctt atgttttagt
      181 ttgattagct ttaggggttt ccagcaattc aagtagtatt ggataggttc tatcctatct
      241 ctacatatag gtttccctat atgctgacta ttttagctac taactcatga ctaaagtcac
      301 gagtgtgcgt agctattttt taatcaatat tttagtaaat tttacggtaa gtaaaaacct
      361 ctctccttca aatcctatag caaatatact tacaagttgc tttgagaaaa taactcctaa
      421 tatagctaca actatgcata taccaactat tatatttaaa acattgttta aaaactttag
      481 tgcttgcttt tcaccttgtt tactacttat atcttgatac attggaataa aagtagtaac
      541 tatagcagta cctattgctg caaatataat atttggtata ttcatagctg ttaaataact
      601 ctctgtatat aagcctgtac cataaattga tgataatact aattctctac ccattccaag
      661 aatcttagag attatagtaa ctatcatcaa ataaaaagtt gctttagcta ccttgctcat
      721 tttatcactc cacttctaac atacctcttt ttaatcaagt aaaatttagc tcattagata
      781 taattctatc aactaatttg tcttgcttag ccctatcaaa atataattaa cacctatttc
      841 ttaatatatc acaaactaca ttattcttca tattttttta tttctccaat aagttttaca
      901 tctttcctat ataaattgta tagcttcaat tatattcaaa gaaatggagt tgctacccga
      961 taactttaaa agtaaaaaac agattttatt atctttttaa actatattct aacatatttt
     1021 gtccatattg agaaatttta ctaaactatt atgtacttta ttaaataaat aaaataacag
     1081 cacacctata aaatacaagt gtgctgttca taaacagatt ttataaaaaa ataattccta
     1141 agtagagatt aacatgcttt ctcatactct catatataat gtgtataaaa atattaataa
     1201 aaatgcttta attgttttaa cttacttgga tgtcttaatt ttcttattgc tttagcttct
     1261 atctgtctta ttcgttctct ggtaactcca aatacctttc caatctgttc taatgtttta
     1321 gggtcatcat catcaattcc aaatctcatt cttaaaactt gctgttcttg ctctccaaga
     1381 cctgtaagta gttcttctat cttttctttt aaattatgct gttcaacttc gtgtattaca
     1441 acatctttaa aatcagcatc tgatggtata aattcaacta aactactatc ctcatcttct
     1501 tttaatggag tatccaaaga tacaacattt ttatctcttc ttaataattc caacactttg
     1561 tcaacttcta agccacatgc atctgcaatc tcatctatag ttggctctct taataataca
     1621 tttaaaagtt cttgtttctt tttctttaca aaatttattc tctgatgaag atgtataggt
     1681 atcctaatag tattttcgca atcgtcgata tatcttgtta tactttgttt tatccaccaa
     1741 gttgcataag tgctaaattt gtatcctttt ttataatcgt acttttctac agcttttata
     1801 agtcctatat ttcctgcttg aattaaatcc aacatatcaa tactatctct tttatatctc
     1861 tttgctatgc tcactaccaa cctataattt gaatttataa atttttcttt tgctactgaa
     1921 gatgagttga ttatttcctt agccagctct acctcttctc ctgctgacaa cattttgtat
     1981 tcctctattt cctttaaata cattttcatc gcatttgata catttgatga acccacacaa
     2041 atataatttt cataattatt ttctttttta gtatccataa tcaaacccct tctattcata
     2101 gaatttgcta aaacaaaaat aaataattta aacctacata atctacaatt acataatatc
     2161 ccattcttta aatgaattgt tttgtatatt atgtatacaa atttataaaa actataccta
     2221 taatattatt ctacttctaa cagcaatatc ctccttatat atataattta ttttattata
     2281 tgttgttata ttcttagttt tttgaatgat atatgtctaa aaaaagactg tttactaaac
     2341 agtctttttt tatatatatg ctcaatattt aattttcatg atgatgatta catccacatc
     2401 cacaattttc atcatggtga tgatgttctt ctaaatcttt tgcctgttgt tccatttcat
     2461 gaaactcttt taccattata tcataagttg gaagagctgc ctcagtatcg tattcttccc
     2521 ctctccaatc agcgtacatc atagcatctc cattagttaa aaccaatcta cctgacatag
     2581 ttatattatc cacttcttct aaaaattcta tttgtttttg taaaacttct ccttctaact
     2641 ccacatcttc atctaaacat tgcactatca ttacaggtgc ataataatct tcttcatctt
     2701 taacatttac aacaatatct aaaattcctt ctgcaccttc ttcaaattct gccaactcaa
     2761 catttgtatc aatcatatct tgaggtacta ataaatcttc tattaaatat tttattactc
     2821 tatcttttac taattctttt gacatttatt tttctccttc cgattctata acattatcat
     2881 aatcatttct atattatagt atactacatt aattgttttt tcaatctctt acaaaaatta
     2941 tttttttatt atcttcctta tattcccttt tttattacaa agttgtaatc gcattgattt
     3001 aaaaaatagt ttatatttag tataatagaa atgtagcaca tatttttatt caatatatta
     3061 ataaaaaaat atattgaaaa tgtaatgtat gttaaatcta ggaggaatat tatgaagttt
     3121 aaaaaacaaa ttagttctat tgttgtgtct actatgcttg tacttgggag tatgaacctt
     3181 tcttatgcag acggtacaaa tgtagttaca cttggagcta acttatcaga gtctcaaaag
     3241 cagcaaatgc ttaattactt tggagtaaaa aaagaccaag tcttagtttt agatgtaaca
     3301 aatgcagaag aaagacagta tttacaaggt gttgcaacag agggtcaact tggaaaagtt
     3361 actatctctt gctcatacgt tgaacctact aaaaaaggaa atggaattaa tgttaaaaca
     3421 gcaaacttaa cttgggtaac tagttcaatg attgcttcaa cccttacaac agctggtctt
     3481 gaagatgcaa atgttattgc tgctgctcca tatcctgtaa gtggtactgg agcattgaca
     3541 ggaatcatga aagcatttga ggatgcttca ggaaagaaac ttgatgaaac taagaaagaa
     3601 attgcttctg aagaattggt agttacaggt gacttaggag atgatatagg gcaagaaaaa
     3661 gcaacaggtg taatgaatga tataaaaaca gaaattgtaa agaatggtac aaaagatacc
     3721 aatcaaatag caaatacaat aaataatgtt gtaaataatt acaatattac tattacacca
     3781 gaacaaaaag aacaaattaa aggtgtaatg aaaaaaatag ctgaccaaaa ttacgattac
     3841 aatagcatga aaaatacact agataatgta tcagataatg taaatgaaca attaaagaaa
     3901 ttaggtgaaa gtgttaaggg ttctggtata ctagatacta ttggtggatg gtttagtgga
     3961 ataggagact ggttctctgg tttattctca ggcggagata aaaaagattt gggaatctta
     4021 aataacacta atgatgagtc tcttggctct gatgctgtaa taaattctac agaaggtgtt
     4081 caaataaatc cacaaaataa tactaattct aacaatgata gtgatacaaa tagctcaaaa
     4141 aatgatactt caagtaataa tgaaaataac gatagttctc aaacaaatga aaacaacaca
     4201 acaaataatt cagaaaatca atcaaataat aatgactcag ttggtttctt tgaaaaaata
     4261 aaaaattggc tttcaggctt attttagaaa ttaaacaaat aaaaagaatt gcctaaaatg
     4321 gaaattttct ataaaaggca attctcttta tttacaataa atttttatta ctcactcatt
     4381 tttttagatt tttctataca tgcacatatt gcttttatca cagaagctct aaaaccttct
     4441 tcttcaagta ctttaactgc ttctatagta gttccaccag gtgaacatac catatctttt
     4501 aattcaccag gatgttttcc tgtctcaagt accatttttg cagaacccat aacagcctgt
     4561 gatgcaaact tatatgcttg tggtcttggc attcctgcaa ttactgctgc atctgctata
     4621 gcttctataa ataaaaatac atatgctggt gcagaaccac ttgcaccaat tacagcttct
     4681 ataagatact ctggtactac ctcagtattt ccaaaagaat taaatacttc tgtaactgct
     4741 tctaagtctt ctttgtttat atttttattt atagaaagag aagacatggc ttcatttaca
     4801 agagctggag tatttggcat agttctaact atttttttat cttctcctat aatagactct
     4861 atatctttta tagattttcc tgctgctatt gtaactataa ttttattgtc atctataaag
     4921 tcttttatct cttccaaaat atcatcatat atatctggtt taacagctac aattactata
     4981 tcagaggttc tagttacttc cttcgagtct tttgtagtat ttattccaaa atttgcatct
     5041 attctatcga gagttccttg actatatgct gatgctataa ccatattagg ttcaactaat
     5101 tttgaattta ctattccccc tatcatagcg cttgccatat ttcctgcacc tatgaatcca
     5161 atttttttca ctttagtacc tccttattga ctaatagtaa tcgttaaaat ttaagcttct
     5221 ttaactgcat cagacttttc aaatggattt ggtatactta ctaccaaaat tttcactacc
     5281 ttattagtat agttgaccca attatgattg ttcttttcac tattatagtg tattgtgtct
     5341 cctggataca tttctatttg ttcatctccc aaaaacactg taagagtacc ctctaataca
     5401 tacacaaatt cctcaccttc atgtacgtaa caacaaattt cctcactact cttacttgga
     5461 agaatttcaa ttaatcttgg tagcattgtt ttttccttta aactagatga taaatgataa
     5521 tgtataaatg ttgagttttc tacatcaaat acttcttttt cataacttct taatacagct
     5581 cttttatgct cttttggctt cataaaaaag taagttaatt ctacatctag tacacttgct
     5641 atttttccta aggaatctgt tgccacactt gttaatcctc tttcaagttg tgataaaaaa
     5701 ccaattgaca agtttgtttt ttcactcata tccttcagcg tcatttgctt ttgagttctg
     5761 agttgcttta tttttgctcc tatgtcctta ttcaaaacca tcacccctaa ataattttaa
     5821 ttttttcata atataaatac aaaaatataa atataggtac tgaacctatc tttatttcat
     5881 ttaaaaaaat ttttataagt attactataa tttatcaatc tttttttgtc tacattttta
     5941 cgattattta cgatttattt ttcacatatg tatatttaat cttgccaact aaagatacat
     6001 tttttataag catttactct ttatataata aaacttaagt tgttcatgta gtcttcttct
     6061 ggtttaatta ttaaaatttc aaattatatt attatttcta cacattatta catcctttag
     6121 tacaattata acataatttt acaatttaat gatatctatt caatgtcttt ttttactcta
     6181 caacatattt tattttagag tttaatacta tatatatttt ttatatacag ccttgtcatt
     6241 ttttagattg tcaagagtta ttgaagtatt ttatgtagat ttagattcta t
//
LOCUS       DAGSWI010000183         5994 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.187, whole genome shotgun sequence.
ACCESSION   DAGSWI010000183 DAGSWI010000000
VERSION     DAGSWI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.187"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..676
                     /locus_tag="MJF80_003571"
     CDS             <1..676
                     /locus_tag="MJF80_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBZ0282180.1"
                     /translation="LNATISKEPSGRYYVSLCCTDVDIEAFENTNNQIGLDLGIKEFC
                     ISSCGEFIENPKYLKKSLNKLVKLQSELSRKTIGSLNRNKARLKVARLQEHIANQRKD
                     FLQKLSTKLIKKNDIICIEDLQVKNMIKNHKLSRSISDVSWSEFIRQLEYKANWYGRQ
                     IVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASRNILKEGLRL
                     ITIQNK"
     gene            803..1972
                     /locus_tag="MJF80_003572"
     CDS             803..1972
                     /locus_tag="MJF80_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBZ0282181.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(2135..2677)
                     /locus_tag="MJF80_003573"
     CDS             complement(2135..2677)
                     /locus_tag="MJF80_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBZ0282182.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2825..3109)
                     /locus_tag="MJF80_003574"
     CDS             complement(2825..3109)
                     /locus_tag="MJF80_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBZ0282183.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(3172..3936)
                     /locus_tag="MJF80_003575"
     CDS             complement(3172..3936)
                     /locus_tag="MJF80_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBZ0282184.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(4218..4484)
                     /locus_tag="MJF80_003576"
     CDS             complement(4218..4484)
                     /locus_tag="MJF80_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBZ0282185.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(4526..5335)
                     /locus_tag="MJF80_003577"
     CDS             complement(4526..5335)
                     /locus_tag="MJF80_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0282186.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            5648..5929
                     /locus_tag="MJF80_003578"
     CDS             5648..5929
                     /locus_tag="MJF80_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282187.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT         2170 a          764 c          857 g         2203 t
ORIGIN      
        1 acttaatgct actatatcta aagagccaag tggtagatat tatgtatcat tatgctgtac
       61 tgatgtagat attgaagcat ttgaaaatac taacaatcag ataggtttag atttaggaat
      121 taaagaattt tgtatctcaa gttgtggaga atttatagaa aatcctaagt atcttaagaa
      181 gtcattaaac aaacttgtta aattacaaag cgaattatcg agaaaaacaa tcggtagttt
      241 aaatagaaat aaagcaagat taaaagttgc aagacttcaa gaacatatag ctaatcaaag
      301 aaaggatttt ctacaaaaac tatctactaa attaataaaa aagaatgata ttatttgcat
      361 agaagattta caagtaaaga atatgattaa aaatcataaa ctatctcgct caatttctga
      421 tgtgtcatgg tcagaattta ttcgtcaatt agaatacaag gctaattggt atggtagaca
      481 aattgtaaag gtaggtaaat tctttgcaag ttcacaaata tgtaataaat gtgggtataa
      541 aaacgaggaa gtgaaggact taaatataag agaatggatt tgtcctagtt gtaatgaaac
      601 tcacgataga gatataaatg caagtagaaa tatattaaaa gaaggattaa gactaataac
      661 aattcaaaat aaataacgta tgtaagaacc gtaggaacta cggggatagc ctgtggagaa
      721 ttagtaagac atattttagt atgcaaaatt ctgtgaagca ggaaccctgt gactttagtc
      781 atgggaggtt cagggtagaa agataaatga actttgtaaa gatgaagttg tagagttttg
      841 caatagaata aaaggtaaag tggaaagtgt tgcaataagt ggagtatttg ctccagtaaa
      901 taaacaacaa gaagaagaag ttgcaaaatg ggttagagaa attttagggg atattcctat
      961 atctttatca aatgaaatag gtaatatagg tatattagaa agagaaaatg gagctatttt
     1021 aaattctgca ttgtctcaag taggtaaagc tgtatcttta ggatttgaaa aagcacttaa
     1081 agacttaggt atagatgctc aaatatattt cagtcaaaat gatggaacat taatgaattt
     1141 agagtataca atgaaatacc caatttttac aattggatgt ggaataacaa attctataag
     1201 aggagcttcc ttcttatcaa aagtaaaaaa tgcaatagta ttagatgttg gtggaactac
     1261 aagcgacttg ggtgtactat ataatggatt ccctagagaa tcatctattc cagttacaat
     1321 tggaggagta catacaaact ttagaatgcc agatgtttta actattggtc ttgctggtgg
     1381 tacatgtgta aaaggcgaaa aagataatat aaagataggt cctgaaagtg tgggatatag
     1441 aataactgaa gatgcaataa tatttggtgg agacactcta actttatcag atgttgttac
     1501 aaaattagat atggcgtttg aagatagaaa aagcaatgtt gaaaacttag ataaagagta
     1561 ttgtaatgag gtatataaga aggttatttt aaaattagaa gatgctgtag accaaatgaa
     1621 gactagtcaa gatgatgttg aattggtttt aactggtgga ggaagtataa ttgttcctga
     1681 aaaactaaaa ggcataagca atgttataag acctcctcat tatactgctg caaatgctat
     1741 tggtgcagct ttaggacaaa taagtggaga tgtagaaaaa gtatacagtt tagataaaat
     1801 gtcgagagaa gatgccatag aagatgcaaa aaaagaggct gtaaataaag ctctaaaagc
     1861 tggtgctaaa gaagatacta tagaagtatt aaatatagaa gatgttccac ttgcatacct
     1921 tcctggtaat gcacttctta taaaagtaaa agttgttggt gatttaattt aaagaataaa
     1981 tataagtatt tccagaatta tataaatatt tctggactca tttttaaact tattttatca
     2041 ataaaaacag cataactttt gactatattc ttataagttt ccgaacagcc gatatatacc
     2101 tattttttga ggtttatcgg ctgtttgtca tcttttatcg cattccaact atttttctta
     2161 ccgtatttgc tgtacgaatt gtaatcttat cacttacatt tgaactggca gtctttgacc
     2221 accaatttgt ctttttataa tctttacgac taaaagacca aaagatagca tttttataat
     2281 gatatacttt ttcatactct tctttaggat ttccaacctc atcataaaac tcgtcataag
     2341 ataagtcagg aaacataaaa ataagattat catatatttc tttgttgtta tctccccacc
     2401 aattaggagc attatttagt atatctttta actcctcttg tgaaataata aaaactggta
     2461 tttccaattc aaactgtttt tttatcatta atcttattgt atttgagata actttgctgt
     2521 catctacatc acttaaaaag acaacattac cactattaag gtatgtagaa acttctgaat
     2581 aaccaagttc tacaaagctt gcttttaatt cagacataat tatcttgttc ttaccactta
     2641 tattgatacc tcttaataat gcgatataac ttttcatata ataatttcac ctctaaacat
     2701 tcacattttt ctatgtagta caaacttgaa tttgcatctt ttataattct ttcaaaactt
     2761 cttcatatgt aattttttta atataaaaaa atcggtgctt aatttgtacc gatttttgtt
     2821 tttcctagta aagaactcgt ttttttgctt ctgctatagc tctagcagca ctttttgctt
     2881 gcattctttt ttcttcttct tttttaaatt catttattgt ttcatttaga ttcttatcat
     2941 gttcattttt ttctttatct tcgttttctc tgtatactaa tatataatct attattgatt
     3001 cgtttattat ttgagatata cttttatttt cattaattcc tattctctta acttccttaa
     3061 gtagagtctc atcaatatga accgttgttt gctttgtatt ttttgccacc ttaatcacct
     3121 cttaaaaaaa attctaacat taaaattttt tttagtcaat aaattttaat attaaaattc
     3181 tttaaatgtt ttatttgatt ttctaaaatt ttctattgta tttatcatct tattttttat
     3241 attttcacct agtacagaag actttagtct aggattttcg atatggtcat aaattggttc
     3301 taaaaattcg acttcgacag gaacagagtt aattttacca ataggttggt atccttcata
     3361 agtatctttt gaatttttta ttactagtgg aacaataggg cattttgctt tatatgctat
     3421 ctttaaagct ccacttctaa actccgaaac caatgaattt ggttctttaa tccatgttaa
     3481 atctccttct ggaaacactg ccatactttg acctgtaagt atattttgag aggcttgggc
     3541 tatactcttt ataccttcac gattgttttt tctgtttaca tatacacatc ttagtaattt
     3601 tgcccattct ttaaatatag gtatatttct ccaaactggt tcatctgcaa ttacacatcc
     3661 aattggtctt tctacacttg ctgttagtat gaagctgtca agcatactag aatgatttac
     3721 aacaaataga agtggttctt ttggcagtgt ttccttacca attatattaa gctctatgtt
     3781 cactatatct aaagattttt tagcttgttt ttgcataaat tcaaattttt cctttaagct
     3841 aaatttatca ggattctttt taattttaat aagcctaggt atagaagatg taaaacctat
     3901 aagttgatat gtagcaaatt ttaagtaatt attcataaaa ttatctcctt aaaataaaat
     3961 tatttattac tgtaaagatt ttaacataaa ataaaattta agtctatttt aattcaattt
     4021 tgtaacatat attttaaata gacagagatt gagattttat atttccaatt ttagtaatca
     4081 gattttatca ataaattact attctattaa tttaattaaa taacctttaa atttaattat
     4141 aaaaactgtt gcagaaattt ctacaacagt ttaaaaaatt taccttacaa aaacttattt
     4201 tataacaaga gagtatatta taaggctagt acagcccatt ttttgcactg agaaattctt
     4261 ttgccatcat caccttgttc tttgtcatat gcaaattgat taagcatttc acatggaaat
     4321 gtatcacata gaccacaatg tgtaaggttt ttatcttcac aacaagattt tacaggacat
     4381 tgttcacccc aaaatggttt ctcaatctct gtacaacctt tacaattgac tttttccttg
     4441 tatccacatt cataacataa aattccacat cttgattcta tcattttaag tcactcctta
     4501 aatataaatt ttatatgatt gtatatcaaa atttatactt ttcattgtaa aaatcggaca
     4561 aattttttat ataggttgtt ggattaacac cacaaataga tttaaaatca tttataaaat
     4621 gagattggtc gtaataacca atatcttgtg ctaaagttgc aaagggtatt ttattatttt
     4681 gtatttcttg taatacaagg tttatacgaa gtaatctaag gtatgattta acactcatac
     4741 caagagaatt attaaaaatt ctatttaaat gtcgttcact ataacaacta atttgtgaaa
     4801 tgtttttaac agacataata gagttctgtt ttctagcatt ttcaagaatt ggtatagtca
     4861 tatctactat atttgatttc aataagtatg ataagaataa catatctaat tgttctacta
     4921 attcctttat agtacttgtt ctttcaaaaa tggaattaac ctctaagcta aataatgtgt
     4981 taaaatcttc aagtgaaatt tttaaatctg tagattctct ttgagaaaga cctgtcaaat
     5041 aatatactcc acctgggcga aactcaacga atactctaaa aagtacattt tctatatcat
     5101 tttttacaat agtagttttt gttgtggctc cccagaatgc gctttctata ccttttttgt
     5161 caaatttaaa tatcatacat ccactagcat caggtattaa tgtaagattt tctttaatac
     5221 ttgtatctgg aatagatatt gtatagtgtg ctatatactg tcttaacaat ttatgtggca
     5281 aaatatatgt atactgtttg caggtattta aaatgttatt gattatttta ttcataagta
     5341 tatcctcctg atatataaaa aataagtata ctaatttatt ttttatataa taaagcagtt
     5401 ttgttttaat tgaaatattc taataagata ttttgttaaa tttagataca ataattgtaa
     5461 agtaaaatct caaaatatac aattattata aatcatagat atttattaaa taaatatatt
     5521 aaagaagaag gaatataaaa caagattaga aaataatagt gttactttca taataaatac
     5581 atataaagat atttttaaga ataaaattta ttattaaata aaaaataaag gagagtgatt
     5641 taatagcatg gcatcaaaac aaataccaag aaacaattta gaaaagtttt taagaataag
     5701 agtcttatca catacaggcg ataaaataca tataaagtta cctgtaaatt ttgcaaagag
     5761 aatgattgag aataatgctt tggatttatt taatggaaaa gatgatgtag tagatggtaa
     5821 aaaagttact gaaatattag ttaaggcatt tgactatgat ttaactggag aagtagttca
     5881 cttggagaga aaaaatggag acattattaa agtttcaatt gtacaataga attattttat
     5941 atgaatttta tttttgaata gttataaata attagaaatt tgtaaaaaag tata
//
LOCUS       DAGSWI010000184         5961 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.188, whole genome shotgun sequence.
ACCESSION   DAGSWI010000184 DAGSWI010000000
VERSION     DAGSWI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.188"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(267..2099)
                     /locus_tag="MJF80_003579"
     CDS             complement(267..2099)
                     /locus_tag="MJF80_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282188.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2092..4338)
                     /locus_tag="MJF80_003580"
     CDS             complement(2092..4338)
                     /locus_tag="MJF80_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282189.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4406..4867)
                     /locus_tag="MJF80_003581"
     CDS             complement(4406..4867)
                     /locus_tag="MJF80_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBZ0282190.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5172..5738)
                     /locus_tag="MJF80_003582"
     CDS             complement(5172..5738)
                     /locus_tag="MJF80_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBZ0282191.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1905 a         1091 c          640 g         2325 t
ORIGIN      
        1 ttaaagaata aaattaaata tacattttga taatcacctc caacattttc cttactacat
       61 attataataa aataaacact ttgtaaagac ataaaattat gtggctatct caaaatatag
      121 agaatcctct ttttcattgt taaaatgata aaaatacact ttatttcata cacaaaagag
      181 tgccttatga attaaaaagt tcattttaag gcaccctcat ttattttatc ttattaatta
      241 catgtctatg ctaagatagt tttttgttac attgcttcat cttcttcaaa ttgagaattg
      301 taaagattgg catagaatcc gcctttcttt agaagttcct cgtgatttcc ttgttcaact
      361 atatctcctt cattcattac aagaatcata tcagcatcac gtatagttga taatctgtgg
      421 gctataacaa aacttgttct accttccata agattatcca tagctttttg aatcaatact
      481 tctgttctag tatcgacaga acttgtagct tcatctaata ttaaaatttt aggatcagct
      541 aaaatagcac gagctatagt caataattgt ttttgacctt gagaaacatt acttgcttct
      601 tcatttaatt tcatcttata tccatctggt aatgtcttaa taaatctatg aacatgtgca
      661 gatttagcag cctctataac ttcttcatct gtagcattca acttaccata tcttatattt
      721 tccataattg aaccactaaa taaccatgta tcttgaagaa ccataccaaa catttctcta
      781 agctcactac gattaaaatc tttaatatta tgaccatcaa tcagtattga accgctatta
      841 acatcataaa atctcattaa cagtttaact attgtagttt tacctgctcc tgtaggtcca
      901 acaatagcaa ctttttgacc aggtttaaca tttactgaga aatcatttat tatagtcttg
      961 tttgggttat atccaaagtt gacattttca aaatcaattt taccttctaa accatctatt
     1021 gaaactgcat tttcaacaat ttggacttct tcttcctctt ctaaaaactc aaatactctt
     1081 tcagaagctg ctgctgttga ttgcaataaa ttggcaacct gtgcaatctg agtcattggc
     1141 tgtgtaaagt ttcttacata ttgtataaat gactgtatgt caccaacctc aattgttttc
     1201 ttaattgcaa gccaacctcc taagattgat accattacat atcccaagtt tccaataaac
     1261 atcataagtg gttgcattat acctgataaa aattgagatt tccacgctga attatacagg
     1321 ctatcattta gtttatcaaa ctcttcaatt acttcatcct cacgattaaa tgcttttact
     1381 attgtttggc cactgtaagt ttcctcaact tgaccattta catttcctaa atattcttgt
     1441 tgatatctaa agtatttttg tgatttcttt attattcttg aaattacaaa cattgatatt
     1501 ggtaatatta aaactgccac taatgtcata attccactta tagataacat cattatcaaa
     1561 acacctatca tagttgttac tgatgttata agctgtgtca tactttgatt taaactttga
     1621 tttaaagtat ctatatcatt cgtaattctc gataatattt caccatgcgt cttagtatca
     1681 aaatacttca ttggcaatct atccaatttt gctgaaatct cttttcttaa attataggat
     1741 actttttgag aaattccact cattataaat ccttgtataa aagagaacag tgcacttatc
     1801 aaataaagga aaagtaaaaa ggttaagatt tttccaattg cattaaaatc tatacctacg
     1861 tttcctccag aaactttact cactagccct tcaaatatct ttgtagttgc ctttcctaaa
     1921 atttttggac ctactataga aaacgacgca ctaccaatag caaatacaat tactagaatt
     1981 attgatattt taaatttaga taaatatgca attagttttc gcatagtccc tttaaagtca
     2041 tttgcttttt gtcctgctcc catagatttt cccataggac ctcttcccat tctactcatt
     2101 agctagctcc tcctttgaaa gttgtgataa agctatttgc tcataaactt cgcaattttt
     2161 tagaagctct ttatgtgttc ctattccaac tatttcgccc tcatttaaaa caattatctg
     2221 ttctgcattt aagatagtac taattctttg tgcaactatt agaactgtac tttcagcagt
     2281 ttttgatttt aaagccttac gaagagctgc atcagttttt aagtcaagtg ctgaaaaact
     2341 atcatcaaat atataaactt ctggattttt tgctattgct cttgcaatag aaagtctttg
     2401 tttttgacct ccagatacat ttgttccacc ttgagaaatt tcagttttaa aagcttccgg
     2461 tttagaatct ataaattctg tagcttgagc tatttcagct gctgttctca tttcttcatc
     2521 tgttgcgtct tttctaccat attttaagtt ggattctata gtaccagaaa acaatactcc
     2581 cttttgaggt acatatccaa ttttttccct taaatcatgt tgagaaacat tttttatatt
     2641 aactccacca agtaatattt ctccttcagt aacatcaaag aaacgaggaa ttaaatttat
     2701 aagagttgac ttaccactac cagtacttcc tataaaggca gttgtttctc ctggctttgc
     2761 tgtaaaagat atatttgtta atataggttc ttctgcgtct ggatacatga acgaaacatt
     2821 tttaaattca acaagaccct ttttatcttc cttaaactct tgtgcctctg atttttctgg
     2881 gtcttttatg cttatatctg acactaatac ttcatctata cgtgtagctg aaacggatgc
     2941 tcttggaagc ataatagata ccattgatat cattaagaat gacattatta tttgcattgt
     3001 atattgtata aatgccatca tatctccaac ttgcattgct ccagaatcaa cactgtatgc
     3061 acctctccaa actataagaa ctgtgataag gttcattata agcatcatag caggcatcat
     3121 acatgtcata actctattta caaaaagatt tgttttagtt aaatttttat ttgctttatc
     3181 aaatctttct tcttcatgtt tttcagtact aaaagctctg ataactggaa taccagtaat
     3241 tatttcacgg cttactaagt ttaacttatc aacaagcttt tgaaccagtt taaacttagg
     3301 cataaccact gagaataaca ctacaattaa tgataagata gcaactactg ctactgctat
     3361 aatccaggcc attgaagtat ttgtgtttaa aacctttatc actccaccta tagccattat
     3421 aggtgcatag aatactattc taagcaacat aaccatcaac atttgtatct gttgaatatc
     3481 atttgtacta cgagtaatta atgaagctgt agaaaattca ttcatttcag tatttgaaaa
     3541 tgacataact tttctaaata ctccacttct taaatttcta cctacacttg cggctactct
     3601 tgctgctaaa aatcctactg ttattgtagc taccatacta atcattgcta taccaagcat
     3661 tttagcacca gtaaataata tataattatt ttgagttttt tctgtatcaa tacctatttt
     3721 ctcatattct gaccttacat atgaaacaga gctttgtgtt atcatactct ctggcatact
     3781 ttcaaagcct ttgctcatct ctttagtcaa tgtatctaac tgttcctttg gcattgctga
     3841 taacaattta aatatatcaa catctccact ttgagtagct tgtggtggaa gtttgctcat
     3901 taactgagct ttcatagctt ttacctcttt actatcgccc tcaagccctg atactattaa
     3961 aatagcttta ccaaaaataa catttaaatc atctatagtg tctttgtctt tagtatttaa
     4021 ctcataaaga gattcatctt ttagacctgg atacttatct ttatacttaa cataatcctt
     4081 tttattcagc aaagtataat tatctagtac tttatcctta ctacttttat ccataaataa
     4141 agtaatttta tttaattcac tctctctaat cacagatgga acagagtttt ctactccacc
     4201 ttgttgtata cctacattta ctatattgga tgtgtattct ggtaaagaaa ggtcacatac
     4261 cgcttgaatt accaatagac agactattat tactattgat aatgccgatt tctttaaata
     4321 tttaattaat ttaaacatac atttcttccc ttctgtaata ttaattagtc taataacatt
     4381 aaattgaaaa acattatttt aaactttaaa aatatttttc atcttcatgc ttatgacatc
     4441 ctgcttttaa attatctcgt acttgcatta gaagtcttct aagtactatt ttttcttctt
     4501 cactaaatcc taaaaaaaac tctctttcca attccttgtt aaattcttca actttattac
     4561 atatttctaa acctttgtct gtcaaaaaaa tcctagatac tctttggtcc ttttcatctt
     4621 gttttctttc cacaagtcca gctttttcca tcctctttat cataactgta gttgtagatg
     4681 cttttacttt ccttttatct gctaattctt tttgactttg cccatctttc atctttaaag
     4741 aaaaaagtat ttggggttga ccatgatata agcctaattt atcaaaataa aaatgagttc
     4801 ttataaaata tattcgcatt aattgcaaga aaacatcatt taatttttct tcagaattgc
     4861 tattcatact tgccctcctt attttttata ttacttagtc ggctaagcat tattttacta
     4921 tactgcatgc attttttcaa tatatttata aaaaattcat ataaatttca tttattaaat
     4981 atttttatat catatagtta taattttaat aaataatagt aagattttta attagtcagc
     5041 tacttatatt ttttattgta aagcttaaaa taaacctttt catatatttt caaataaaaa
     5101 agttgtccca aaataaattt tgaaacaaca tcatattcaa cagtatcttg attatataac
     5161 attatgatac tctacttatc ttcatatcca tttacaacaa cgtctaactt cccagtttca
     5221 ggtgatataa ttagaccatg tacaataata ccatctggaa ttaaaggatg cccttttatt
     5281 aaatcaatac tatcttttac agattcttct gcagaatcga atccatgtaa ccattgtttt
     5341 gtatcaattc cagcactacg aagtgtaagt attacatcat tagatattcc tctatctaaa
     5401 attttaccaa gtaacttatc cgtatcaaca ttacacatac cacatccatg atgccctaca
     5461 accataacct catctacatc aaattcgtaa attgcaacta aaacacttct cataatactt
     5521 ccaaaaggat gcattatagt agctcctgca ttttttatta gtttcacatc tccatttttc
     5581 aaattcatag ccttaggcaa aagctcagtt aatcttgtat ccatacaaga taagactact
     5641 atttttttat ttggatgttt tgatgtcaca tattgttcat attctttgtt ctctacaaat
     5701 gatttattat attctaatat ctcttcaagt tttctcatat gtaatatcac cctctttaaa
     5761 aattttttta ttattatatc actattaaag tacaatatat acacatataa tttaatttat
     5821 atttattaaa ttattttata agattattac tctactataa atcttgtata taacttgtat
     5881 ttataagtat ttgtatattt attttttata atctaaacta atattatata ttttcattat
     5941 ttttttaatt ataatttttt t
//
LOCUS       DAGSWI010000185         5897 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.189, whole genome shotgun sequence.
ACCESSION   DAGSWI010000185 DAGSWI010000000
VERSION     DAGSWI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.189"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            128..952
                     /locus_tag="MJF80_003583"
     CDS             128..952
                     /locus_tag="MJF80_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0282192.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            1051..1659
                     /locus_tag="MJF80_003584"
     CDS             1051..1659
                     /locus_tag="MJF80_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0282193.1"
                     /translation="MGKFLRGKIMKSDSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            1763..1939
                     /locus_tag="MJF80_003585"
     CDS             1763..1939
                     /locus_tag="MJF80_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282194.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            3030..3782
                     /locus_tag="MJF80_003586"
     CDS             3030..3782
                     /locus_tag="MJF80_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282195.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            3772..5883
                     /locus_tag="MJF80_003587"
     CDS             3772..5883
                     /locus_tag="MJF80_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282196.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCTVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISVVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         2208 a          774 c          805 g         2110 t
ORIGIN      
        1 acttaaatat aatttactct ctataattta ttgcaacatc tggaaaataa ttgtatttcc
       61 acaaacgctt gaagtatata aaaacaaaat atatactgat tataagtgaa aaggaggtca
      121 tattttcatg tttaaaatag gagaggtttc aaaactaaca caaatttcta ttcgtatgtt
      181 gcgttattat gatgaacttg gtattttaaa gcctgcagaa atagacaaat atacaggtca
      241 tagattatac tcagttgaac agatttcaat attacaaaga attgtattat tgagagattc
      301 aaaatttagc gttgcagaaa tagcaaatat cgtacataat tggaatgatg aatttgtaat
      361 taaggaattg aatagaaaaa agaatgaaat acaaaaggaa ataaagcagg aacaacaaag
      421 aataaataaa attgataaat ttatagaagc tataaactgt gataaagatg aaattcatta
      481 caatgttgtc tttaaaaaga ttccaagtta taaaatcata tcacttagag agataattcc
      541 agactaccaa tctgaaggta tactctggga aaagctatct aaatttatca aagaagaaca
      601 tatagaagta tcacgtcaat ccaacaataa catagctttt taccatgatg aagaagtcaa
      661 agataacggt gtagatattg aagttggaat ggttgtaaag aagattggaa agaacaaatc
      721 tggatttata tatcgtgaga cagaagaaat tgatatgatg gcatgtacta tggtttatgg
      781 tccatatgaa aatatagctg gtgcatatga atcgttctgt tattggttag ataaaaatag
      841 tgattatcaa atatcaggta taaatagaca aatcggtcat aaaggtgaac ataatgaaat
      901 aaatcctgaa aattatctta cagaaattca aatccccctt ataaaagtct aatcgattag
      961 tacttaaata cttaagtact aatcacccat tgacatccca aaattttcat gatagtatct
     1021 aaacgtgtat taaacatgtt taattttttt atggggaaat ttttaagggg aaaaattatg
     1081 aaatcagatt caacaaagga acaattaatt aatgctacaa tccagttact atcttcacat
     1141 agagatgttt caagcattac ggctagagaa attgtagcta aagcaaatgt caatttagct
     1201 atgattaatt attattttaa atctaaagag gagttaatta atatttctat taaaaaattt
     1261 ttaaagcgtc atatggatga atataaggct acacatgata ctaaaaattt agcacctaaa
     1321 cagcaattaa gggaaatact aattgcattt tttgatttta taggtgaaca tagtcaattt
     1381 ataaagattt ctgtacccta tatattgata caggaagaaa ttgtttatcc atttgagata
     1441 ctacctttaa ttaaggctca ctatgaaaat cagaataaca aaaaaactga agctgaatac
     1501 agaactattg cgtatcaaat aacttcattt attcagttgc tttttcttcg ttctaatgct
     1561 tttttcaagt atagtggtat tgatattctt aatgttgaac agagaaatgg tttaatagat
     1621 tttcaattag atttattatt ggtagatatt acaaaataat actatattaa ttttttatgt
     1681 acattttttt attttatggt aacgtttgaa aatattctca ttgactaaaa atacttgcat
     1741 taaataaggg ggaggaattt taatgttttt tacatttata ggagtaatat cagcaatttt
     1801 attgttgtgt acaattatat gtggtttatg gataaaactt aataatcgta ataatgactc
     1861 taattctcac tttacttttt caatactttc aattgctttt tcactgttaa ttgtggtggc
     1921 ctttttagta agaaattaat gtctttgtta tgctggggtt tattattata gatttttatt
     1981 attgcatgat ttttactata ttatagtatt tttattatat tacagggtct tatttattat
     2041 agtgttttgt tgttacagca ttttattata ttacagagtc ctatttatta taggattttt
     2101 attatattgt aaaatcttat ttattatagg attttgttat attacagact tttattatat
     2161 tgcaatctct tatttattgc agtgttttgt tattacagta tttctattat attgcaaact
     2221 cttatttatt atggtgtttt gttattgcaa catttctatt atattgtaaa gtcttattta
     2281 ttacagttct ttattatact acagaccttt attattacag catttctatt acattgcaaa
     2341 gtcttattta ttacagttct ttattatact acagactttt attattacaa cattttatta
     2401 ttttaacttc tttactataa tatagtaata ttttaatatc tcaaaatcat atcaatcact
     2461 tttcagcata gtctaaaata cttccacttt ctaaaattat ctatcatacc tatactaata
     2521 atacttttta acctttttat tatacatttc aattgttttt aacaacctat ttatttccaa
     2581 aactccaata tattttctca attacattgt gttaataacc cctaaatcct ttcaaattat
     2641 ttacaataca ttccattaaa taatcttact gctctataat tctttttttc tttttaaccc
     2701 tactatacat tatttacatc atttttttca atacaacatt cgttattcta taaccttttt
     2761 acttttacta tatacctctt ttctaactac accttcaatg ttccacatca tattgcttta
     2821 gcacaacaat taatacctaa aaataaccct ctaaatcctc caataatata atcaatttaa
     2881 ctataataat aacctctcac actatccaaa caatatattt tcaaacatta caaaaaaaca
     2941 tccaactatt acaatatatc tcatatccct cctccccttt caaatgctat cattgactta
     3001 caagataaat gaaagtgagg atgatatata tgcttaaagt aaaccaatta tgtaaatcat
     3061 ataaaacagg cagtaaaaca tatcctgtat taaaaaatat ttcttttgaa atcaacaaag
     3121 gagaatttgt cgcaatcatg ggttcatcag gaagtggaaa aactactctt ttaaattgta
     3181 tttcatgttt tatcccaatt gataatgggg aaataattct tggaaataat aatataagaa
     3241 atttaaaaga aaaggaactt tcagaagtta gaaatcaaca actaggattt gtattccaag
     3301 atttcatgct atttgatggt cttagtgtat ttgaaaatat atgtgttcca caaattatca
     3361 aaggtagtca agtcttacaa atggaaaacc gtgcaattaa tctatgtgag ctatttggta
     3421 ttgaacatat cagaaaaaaa taccctgccg aaatatctgg tggagaaaaa cagcgtacag
     3481 cagtagcaag agcactcatg aacaacccag atttaatatt agcagatgaa ccaactggta
     3541 accttgatag taagtcttgt cgtacagtca ttgaatcatt tttaaaagca aagcaagaat
     3601 tagatgctac tatactcatg gttacacacg atagctttgc agcaagcttc tgtgaccgag
     3661 ttattgtgct taaagatgga ataatttata aagaagttgt aaaatcaaat tcaagacata
     3721 aattcctaga tgaattacta gaagttttaa aagaaatggg aggcgctcaa gatgacaatt
     3781 aataaattat acagtaaact tcgagcctat aattggaaga actatacaac tttattcttt
     3841 tgtataatac tttctgtggt attagtaact tcatatgctc taatttattt tagcccaaca
     3901 atacaaaaca tacttccaac tggtggagat agcagaaaac aggctacaat gatattctca
     3961 gttgctatat taggatgcac tgtctttact acatacgcat cttccctctt tttaaaattt
     4021 aaaagcaagg aaattggtat tttcatggct ctaggaacag ataagaagaa attaaaaaaa
     4081 cttctatttt cagagatatt gttaatttca gtagtatgct gttttttagg tcttatatta
     4141 agtattcctg tatcttttgg tatatggaaa atatttcaat tagtgattat tgataccaga
     4201 gaaatgacat atcattttgg aactcaagga ttcatatatg gatttatatt tagtgtattt
     4261 gtgacattat gcatatttat actttctgta aaatttatta atcgtacaaa tataattgat
     4321 atattaaatg accagagaat atctgaacca atacaggatg taaaggactg gtatggtata
     4381 ttaggtataa tttttattat actcggtatt tttctaggat atctcgtacc ttcactaact
     4441 ataaacaaat taaattataa actaccatct atatggagct ttacttatct actctctctt
     4501 attggaatat atatgattat gacatacata gttgtacatg ctaaaagagg aaaacatcca
     4561 gagaaatact ataaaaacat catatcaact agcatgatga gatttatggg aagacaaaca
     4621 gtaaaaaaca tgtgtgttat ttcatttttg attgcaggag cattatttgc tgtattctat
     4681 gttcctaata tggttacagg tatctatgat actcttaaaa ataaccctat agattatact
     4741 tttacctaca atcaaagaga taaacaggta tcaaaaaaag atattgaaca attagcagat
     4801 aaacacaata taaaaattca aaactatatg gaaattcctt ctatagaatt aattgttgat
     4861 ggtgtagaat caatatatca taaagatgga actatcgaaa aggtatatat gaaaaaaagc
     4921 tcctatgata tgttctttaa ttccaaagat ttttccaagc taattggcaa accaataact
     4981 ataaaaaatg gtgaatatct tgctgttgta aataaaggaa atgaaaatca agattataaa
     5041 tataccaaat ttatatcaag cccagtcaca gaaaaagaaa tcccctttag atatgctgga
     5101 aaaataatat ttaatggttc tttttttcaa aatagaagta tgaacgccta tgtccttaat
     5161 gaggaggatt ataaatcatt tactaaagat ttatccataa aaaattacaa taattctata
     5221 ctatttaatg tagataaaga tacctatgct tttgccaatg acttaaagaa agaaattata
     5281 aaaagaacat ctaaggaaat gggcatgttc agtggttatg atgaatacgt aaaaaaacgt
     5341 tatgaatcaa ctggaagaaa atattttatg gatgaagaat atccaagtgg tgaaggtcat
     5401 ttaaaactag acccaaacaa caatcagttg tatcttaatt ggaaatacta tcctaacttt
     5461 aaaattttac aaagccaaga tatgataaaa aacatggcag tcttcttaat gttattcata
     5521 tatgtggcaa tcatatgttt tacagcagta ggaatcattg catatacaag gggtgttact
     5581 atagctataa atcacaagca ggtctttatg gatttaaatc gattaggagc aaacaatgat
     5641 tatataaagt tctgtattag aaatcaatta aagaaagtat tttcataccc tcttattgca
     5701 ggaagttttt taatttatct gtttaatttt atgattatgt taaataatga tggtagaatc
     5761 gtcaatagtg aggtcaatgc tcttttgata aacttaggtt ttatagggat ttgtggggtt
     5821 tatatgtacc ttatatatct attaacttat aagaaattca aaaaaattat tggtatttta
     5881 taataaaaaa ggttatt
//
LOCUS       DAGSWI010000186         5749 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.190, whole genome shotgun sequence.
ACCESSION   DAGSWI010000186 DAGSWI010000000
VERSION     DAGSWI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.190"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            75..2306
                     /gene="pflB"
                     /locus_tag="MJF80_003588"
     CDS             75..2306
                     /gene="pflB"
                     /locus_tag="MJF80_003588"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBZ0282197.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            2466..3200
                     /gene="pflA"
                     /locus_tag="MJF80_003589"
     CDS             2466..3200
                     /gene="pflA"
                     /locus_tag="MJF80_003589"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBZ0282198.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNIDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3322..4992)
                     /locus_tag="MJF80_003590"
     CDS             complement(3322..4992)
                     /locus_tag="MJF80_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HBZ0282199.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYEEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRINYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
BASE COUNT         2036 a          821 c          823 g         2069 t
ORIGIN      
        1 taaaataaat taagtatata aaaattaatt ttaatagtat tatttatcat ttaaatctaa
       61 ggaggaaata cttaatgaat gcatggcaag gatttaaaac aggaagatgg actaaagaaa
      121 taaatgttag agagtttata caattaaact attctccata tgagggaaat gactctttct
      181 tagctggtgc cactgaaaat actaaaaaat tatgggacga agcaatggtc ctatttaaaa
      241 aagaaagaga aaatggtggt actttagatg ttgatacaaa aactgtttct ggaatagctg
      301 catatgctcc aggatattta gataaagaat tagaaacaat agttgggtta caaacggatg
      361 cccctttaaa aagagctgtc atgccatatg gtggtataaa gatggttgaa aattcatgcg
      421 aagcttttgg atatgaatta gacccagaaa taaaagatat atttactaaa tacagaaaaa
      481 cacacaacca aggagttttt gatgtttata ctccagaaat gagagctgct agaaaatctg
      541 gtataataac aggtcttcca gatgcttatg gtagaggtag aataattggt gactacagaa
      601 gagttgctct atacggtgtt gatgctttaa tagaagataa aaatgagcaa aagaaatcat
      661 tagaagtttc ttgcatggat gaggaagtta tcagattaag agaagaaata actgagcaaa
      721 tatcagcttt aaatgaactt aaaaaaatgg ctgaatctta tggatttgat atctcaaaac
      781 cagcaactaa ctcaaaagaa gctgtacaat ggttatactt tggttattta ggtgctgtta
      841 aagaccaaaa tggtgctgca atgtctttag gtagaacatc tactttctta gatatatact
      901 ttgaaagaga cttaaaagct ggaatcgtaa cagaagaaga attacaagag tatatggatc
      961 attttgttat gaaattaaga atggttaagt tcctaagaac tcctgattac aataacctat
     1021 tctctggaga cccaacttgg gtaactgagt gtataggagg tatgggaata gatggtagaa
     1081 cattagttac taaaaactca tttagaatgt taaatactct atatacatta ggaccttctc
     1141 cagaaccaaa cttaacagtt ctatggtcaa ctaaattacc tcaaggattc aaagatttct
     1201 gttctaaagt atctatagat acaagttcag tacagtatga aaatgatgat ttaatgagag
     1261 catactgggg agatgactat ggtatagctt gttgtgtatc tgcaatgaga attggtaaac
     1321 aaatgcagtt ctttggagct agagttaact tagctaaaac tttattatac gctataaatg
     1381 gtggtgtaga tgagaaatct ggtgttcaag ttggaccaag atttgaacct ataacttctg
     1441 agtacttaga ttatgatgaa gttatgagta aatttgaacc attcactgat tggttagcaa
     1501 cattatatgt aaatacttta aatgtaatac attatatgca tgataaatat tcttatgaag
     1561 cattagaaat ggcattacat gatagagata tatttagaac tatggcttgt ggtatggctg
     1621 gattatcagt ttgtgcagac tcattatctg ctataaaaca tgctaaggtt aaaactatta
     1681 gaaatgaaca aggaatagct gttgattttg aaatagaagg agattatcca aaatacggaa
     1741 ataatgatga tagagttgat agtatagctg tagagttagt tgaaagcttt atgaataaaa
     1801 taagaaagaa taaaacttat agaaattctt atccaactca atctatactt acaataactt
     1861 caaatgttgt ttatggtaag aaaactggta atacaccaga tggtagaaga gctggtgctc
     1921 catttgctcc tggtgctaac ccaatgcatg gtagagatac taatggtgct ttagcttcat
     1981 tatcttcagt tgctaaatta ccatatgaac atgctcaaga tggtatatct aatactttct
     2041 ctatagtacc tgctgcttta ggaaaagata tgactgaaag aataaacaat ctttctgcaa
     2101 tgatggatgg atacttcgct caaaatgctc atcacttaaa tgtaaatgta tttgatagag
     2161 ctactttaga agatgctatg gaacatccag aagaatatcc acaattaact ataagagtat
     2221 ctggatatgc agttaacttc ataaaattaa ctaaagagca acaattagat gttataaaca
     2281 gaacattcca cggtaaaatg gcttagtctt taaattttta tactttaact aagattacta
     2341 ctgtgtaaaa aataaaaata aaaggctatc ttatatttga attatttctg gtataagatg
     2401 gcctttttta gagaaatatt ataactattg ataaggttat taaaatatat tgtgaggtga
     2461 actctatgat taagggaaag atacattcta tagaaacgtt tggtactgta gatggaccag
     2521 gaataagata tatattattt tttcaggggt gtcctcttag atgcaaatac tgtcataaca
     2581 gggatacatg ggatattaaa tcaggaaaag aatacacagt tgatgaaata ataacggatg
     2641 ctcttaagta tacttctttt atgaagtttt ctggtggagg tataactgcc tctggtggtg
     2701 aatccactct tcaaccagaa tttttaagtg aattatttaa aaaagcaaaa gaaaatgata
     2761 tacacacttg cttggataca tctggatttg tagatataga aactatagac ccagttttag
     2821 ataatattga tttggtttta cttgatttaa aacatatggt tgaggaaaaa tctattgatt
     2881 tgactggtgt tggtatggat aaggcattaa aacttgctag acacttagaa tcaagaaata
     2941 ttcctgtatg gataagacat gttttagttc ctggcattac tgatgatata gataatttag
     3001 aaaaactagg tcaatttgta gctacattga aaaatgtaga aagatttgaa ttgctaccat
     3061 accattctat gggaatacac aaatgggaaa gtttaggtat cgattatgaa ctaaaagatg
     3121 ttcctgatgc cactaaggaa gatgtccaaa aagctagtga aataatctct aaatttggag
     3181 tgaaagtata taatagttag tgtagtatat taatccttta tagaagacat aacaaaatag
     3241 ggtaaagtgt aaatttttaa taaacttcaa aacttccact ttgccctatt tttatatact
     3301 atatatctca ttttatatac atcactttga tagctgaaat ttataaaatt cagaggcact
     3361 tacaggtctt ccaaataaaa aaccctgaat aatatctaca cctaagcttt ttactatatc
     3421 atactcttca taagactcta ttccctcaac acaaactttt aaatttataa tatgagctaa
     3481 tttaataata tattcaagaa aagtatattc ataactatta tatgtaatat tcttaacaaa
     3541 acttctttca atttttatta tattaacaga cagttcttta aggtaattta aagatgaata
     3601 tcctgtacca aagtcatcta ttgcaatata tactcctata ccctttaaac taataaattt
     3661 atcatttagt aaatttatat ctggtatcca acaattctct gttaattcca aaattaaaaa
     3721 ttcaggtctt aattgatact ctactaaaca ttctactata aaatctctaa aaaaatcttc
     3781 ctttaactga atatacgata catttacaga tattttaaag tctggattta ttttatgcca
     3841 ttctttacac tgttttacag cttcttttat aatccacttc ccaactggta taattaaatt
     3901 actctgttct agtattggta taaattcaac aggagatact tctccataag tactcgaatg
     3961 ccatcttaaa agtacttctg cccctattac ctcttttgta acagcattta cctggggttg
     4021 aaaaaacaac tcaaaatcat taaaattgtt ttcaacgcac tctcttagtt tttgttgcat
     4081 tgaaattacc tttagtttat tttcatagag ctcttgtgaa aaaaacttaa ttctattttt
     4141 accactaatt tttgctatat ccaatgcaat atcagcatgt ttaaataaat ctaaataatt
     4201 atctccatct tctggataca tagcaactcc tgcagtcact gtgcaatagt acttatttga
     4261 ttcaatttca tgctgagtat ttgtatacat ctgtatcttt tcatatattt tctctatagt
     4321 ttctttagtg cacatagggt aaaaaaatgc aaattcatct ccatctaacc tatacaatct
     4381 tacatctttt ggaagatatt ctaatacttc agtagcaatt gcacgcaata ctttgtcacc
     4441 ataagaatgt ccatatttct catttatatt tttaaaatta tcaatatcca tgataaacat
     4501 agcaccattt ttataaattt tctcttttat aagataattt attctatgtt caaattgttc
     4561 tctattccac gttcctgtga tagaatcaaa tttatttttt tcaccaatat tctttattct
     4621 cccaactaga aatatagttt ttgggtcatc cgacacataa actttacctc tacaagaaat
     4681 ccatactatt tcttcatatt tattaatcaa cctatattct aaattgtgct cacctttttt
     4741 tccatgtagt aattcttgta tatcatcttt ccatatttga acatcatccg gataaacaat
     4801 tttactccaa caattgacca aatcagattc tatctcttta cttaagttaa attcatcaaa
     4861 aattgctgaa gaaattttaa atttattcct atttatatcc caaaaaaata tataatcttc
     4921 tgtactttga gataaaagtt caaagaaaat ttctatatgg tctaaactta aagagttcga
     4981 ttcttctctc atcttttccc cctacaatat tactattagt gtagtttaat caacaaaata
     5041 tccttattta gattatacta ttttactaat tttttatctc atcaaacaca tttccccaac
     5101 tttagttatc ttatcaaatc tcttactctt aactaagttt tttgagattc ataaagtaat
     5161 ttttctataa tcattactgg tgatttattt ttacctgcta aaacaccatg ttaaatcatt
     5221 tattaaatca ttttctctta aaattctatt tttcattata taaaaagttc tcagttcttt
     5281 atcctataaa actcttctat tactcttgaa tttattttat ttttttctcc gcctattctc
     5341 tttggatttg gtattgcttt tctatagatt ttggcaaaga agtacctctt actataatat
     5401 aagattttga tatttcttca tcatctataa attttttaat agggattata tcatttttta
     5461 tatttattta tataaatatt ttttgttttt aattttttct ttcttcttat gtacttacat
     5521 ttaatttatt cttttcagtt aataaaatag gtacattttt agtacatgaa agtagagttg
     5581 cttacagggt atataggatt gatgtactat ttactaatat tacattttca actgattcaa
     5641 cccctctttt acttacataa tttaagtata tttttctgga tttttaaatt tatgtacttt
     5701 aaaatggata tacttatttt cattgatttt gtaagaatta ataaaaaat
//
LOCUS       DAGSWI010000187         5652 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.191, whole genome shotgun sequence.
ACCESSION   DAGSWI010000187 DAGSWI010000000
VERSION     DAGSWI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.191"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..288
                     /locus_tag="MJF80_003591"
     CDS             <1..288
                     /locus_tag="MJF80_003591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282200.1"
                     /translation="KEGTIVETEENGCNGIVEIDKNKQEKSKDSNSVVNNKNEDKKDN
                     LSKQNTSINKLKESPATGDENIIIYIFSILMVIAFLLKKYIISFTKSLLKR"
     gene            648..1355
                     /locus_tag="MJF80_003592"
     CDS             648..1355
                     /locus_tag="MJF80_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282201.1"
                     /translation="MCKIAICEDEKVQVEKICEILKKFELNEGIKLNVDTFSSGEELI
                     ENKFEKYDIVFLDIKMKKLTGIETSKIIRKSSEKIKIIFITALEQYWPEGYTVSAYRY
                     ILKPIKEDELYSIIKDLINEINKNKRFILLKKDGAIEKVLISDIKYLGIENRKVVVYT
                     VNGVYSTSISLGEWESKLYGYDFAKPHSSYLVNFKYIKQVNKNHVVLTDGESVHVSQR
                     KYKEFKDKFIKYINEIR"
     gene            1386..3077
                     /locus_tag="MJF80_003593"
     CDS             1386..3077
                     /locus_tag="MJF80_003593"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019874.1,HMM:NF025854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBZ0282202.1"
                     /translation="MSNMRKKRNIYIAIVLICISSIGVLMYKYFIDNKKEYQERLKDT
                     NEIVIDTQKKRIKEIVDRTINDIELEEKRVINTKEKEIEEISLLFDSFSQDLETEKIV
                     NLVQKVGNKFDENSKVKLIIWNGARKSIEYSNDKNINDNKDINKEDILKYTKDNYILR
                     KVKNIGNSKLLILGVCQSDIDDRVKQIAIKKIRDTKLDEGGYIWVNEILNYNGGDNYA
                     KRLVHPNLIDREGEFLSTNMKDSKGKMPYLKELEDIKAKGESYNEYNFKKVNSNDESL
                     KLSYAKLYPKYNWVIATGVHLDDLQNYNKKYNQEYTEKFKKQFFFRVMNIILLFIYAI
                     ITGGIIFNYNEIRKKMIIKEKNKLLKSHYDILSDKNDDANKIIHDIKNHLITIYTLAK
                     EDGNNKLIEYLYSVGKDIKKYGNKVITENKIVDIVLNEKIELMKKKNIKFTHNIEKID
                     LDFIENKDLVGILSNLFNNAIESAEKCSERKIEFILYSFNKGYVIIKMINTCEKEPIV
                     KGGKLITTKGRKEYHGYGVRIMESIIEKYDGDIEWEYNDEKNIFSVLIMIPIKEI"
     gene            3322..3558
                     /locus_tag="MJF80_003594"
     CDS             3322..3558
                     /locus_tag="MJF80_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0282203.1"
                     /translation="MKVENKNYRLLPYSVILEASKGDISAIEVVLKHYEMYITKLCTR
                     TVCDKQGRTFIYLDENKKSRIELKLITKILTFKP"
     gene            3920..>5652
                     /locus_tag="MJF80_003595"
     CDS             3920..>5652
                     /locus_tag="MJF80_003595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282204.1"
                     /translation="MTRSRKNLKNNRKRLIKNNLTKTITTTVIATGVCAPFSLTNVYA
                     EEIKSVDKQIEAKKIDEKYPETGITGWGLEDENGKVIEGHGSEAKKVDKKYPETGITG
                     WGLEDENGKVIEGHGSEAKKVDKKYPETGITGWGLEDENGKVVEGHGSEAKKTDKFSE
                     QLKVNTEGEKDKIKTQNESNKEYIFEIFDLKKLQEIKDKLDKYKGKLNKELSIGLERQ
                     KSNIEIYIGLFKANNDCSNFKDLVKKPDVDENAYYRILNFINEEYDKFKEACDKYDRE
                     QTGKKSEQGKQPEDKKPDHSELEESEIAIEKPLKDIKDKALSDNARLHSEVDSLLDKY
                     GSVLGLDKSGIAAEFGAIEEIIKNADSYQKILTVGSMSECVKKEYDVTVVSNFLEKEL
                     KKIEKVININTETEENKELLREVRDISKKVLELNKLKGFIRELNGKNDTLIAKDEFEE
                     IYKNIREIKEKYNSIFDEGYVEKARLLYMKLKNGNLEKDQSSIGKERAEVMDKIRKSK
                     NKEELVDILKKPIPGNIDEFDKQYMESILLRTTNDELESQKNKLLDLLSRERGNLRWE
                     GIKEEDKQPEV"
BASE COUNT         2561 a          429 c          984 g         1678 t
ORIGIN      
        1 aaagaaggaa caatagtaga aacagaagaa aatggttgta acgggattgt tgaaatagat
       61 aaaaataagc aagaaaaaag caaagatagt aatagtgtgg ttaataataa aaatgaagat
      121 aaaaaagaca atttatctaa acaaaatact tcaataaata aattgaaaga atctccagct
      181 acaggagatg aaaatattat aatatatata tttagtattt taatggtaat tgctttttta
      241 ttaaaaaaat atataattag ttttactaaa tcacttttaa aaagatagat ttgtaaaaag
      301 taaaggggag ctaggttgaa tttttaaact tagttccttt ttagttttta tagggaattt
      361 atgatttaat gtatcaaatt tattgttatt ttatgaaaat attagaacaa attgataaga
      421 gtgagtggta ttatataatt ataaaaaaat aaattttaaa gttaaattta ttttttataa
      481 agataaagca aaataaaaaa cgtgaaattg aaagcttata caatctatat ttatatagat
      541 tttttagtga gtttataaac tgtttattag tgaaaataat ggtttgctaa ttatattata
      601 ttttgataat tatgagctat aggattttaa ggtaaaggaa gatgagtatg tgtaaaattg
      661 ctatatgtga ggatgaaaag gttcaagttg aaaaaatatg tgaaatatta aaaaaatttg
      721 aactcaatga aggtattaag ttaaatgttg ataccttttc ttcaggagaa gagcttattg
      781 aaaataagtt tgaaaaatat gatatagttt ttttagatat aaaaatgaaa aaactaacag
      841 gtatagaaac atctaaaatt ataagaaaga gtagcgaaaa aataaaaatt atatttataa
      901 cagcactaga acaatattgg ccagaaggat atacagtaag tgcatatagg tatattctta
      961 agcctataaa agaggatgag ttatacagta taataaaaga tttaataaat gaaattaaca
     1021 aaaataaacg ctttatatta ttaaagaaag atggagctat agaaaaggta ttaattagtg
     1081 atattaaata cctaggtata gaaaatagaa aagtagtagt atatacagtt aatggagtat
     1141 attctacatc gatttcctta ggggaatggg aaagtaagct ttatggatat gattttgcta
     1201 aaccacatag tagctattta gttaacttta aatatattaa gcaagtcaat aaaaatcacg
     1261 tagtattaac tgatggagaa tcagtacatg tctcccaacg taaatataag gagtttaagg
     1321 acaagtttat aaaatatata aatgaaattc gttaattaga tatattataa tatgaggttt
     1381 gagatatgag taatatgaga aaaaaacgca atatatatat tgctattgta ttaatatgca
     1441 taagctcaat aggagtttta atgtataaat attttattga taataaaaaa gagtatcaag
     1501 agagactaaa ggacactaat gagattgtta ttgatacaca aaagaaaagg attaaagaga
     1561 ttgtagatag aacaataaat gatattgaat tagaagaaaa aagggttatc aatactaagg
     1621 agaaggagat tgaggagatt tcacttcttt ttgatagttt tagccaagat ctagagacag
     1681 agaagatagt taatctagtt caaaaagtag gaaataaatt tgatgaaaat tcaaaagtaa
     1741 aattaattat atggaatgga gctagaaaaa gtatagaata ttctaatgat aaaaatatta
     1801 atgataacaa agatataaat aaagaagata ttttaaaata tacgaaagat aattatatat
     1861 taagaaaggt taaaaacata ggcaattcta aactattaat tttaggagtg tgtcaaagcg
     1921 atatagatga tagggtaaag cagattgcta ttaaaaagat aagagatacg aaattagatg
     1981 aaggagggta catatgggtt aacgaaatac tcaattataa tggtggagat aattatgcta
     2041 aaaggttagt tcatccaaat ctaatagata gagaaggtga atttttatca acaaatatga
     2101 aagattctaa aggaaaaatg ccttacttaa aagaacttga agatataaaa gcaaaaggag
     2161 aatcttacaa tgaatataat tttaagaaag ttaactcaaa tgatgagagt ttgaaattaa
     2221 gttatgctaa gctttaccca aagtataatt gggtaatagc gactggagtt catttagatg
     2281 acttacaaaa ttataataaa aaatataatc aagaatatac ggagaaattt aaaaaacaat
     2341 ttttctttag ggtaatgaac attatactat tgtttattta tgctattatt actggcggaa
     2401 taattttcaa ttataatgaa ataagaaaaa agatgataat taaagaaaaa aataagttgc
     2461 taaaatctca ctatgatata ctttctgata agaatgatga tgctaacaaa ataattcacg
     2521 atattaaaaa tcatttaata actatatata cattagcaaa agaagatggc aacaataaac
     2581 taatagaata cctatatagt gtaggcaaag atataaagaa gtatggaaat aaagttatca
     2641 ctgaaaataa gattgtagat attgtgctaa atgaaaagat agaactaatg aaaaaaaaga
     2701 acataaaatt tactcataat attgaaaaaa tagatttaga ttttatagaa aataaagact
     2761 tagtagggat tttaagtaat ttgtttaata atgcaattga aagtgcagaa aaatgttctg
     2821 aaagaaaaat agagtttata ctatatagtt ttaacaaggg gtatgtaatt ataaagatga
     2881 tcaacacttg cgagaaagaa cctattgtta aaggaggaaa gctaattaca acaaaaggaa
     2941 gaaaagagta tcatggatat ggtgttagga taatggagtc aataatagaa aaatatgatg
     3001 gagatataga atgggaatat aatgatgaaa agaacatatt tagtgtttta ataatgattc
     3061 cgattaaaga aatttaatta acattagaat gaaacttgtc aaggagaact cataaaatga
     3121 gttctctttt tatttactat aaaggctaat aatatagtga atttaatata ataatttagt
     3181 tataagttaa ttttacataa aagtaaatca aaattacata tagtattgtg tagtagggga
     3241 aatttattat actaattata aatagatgat tttatagata aaaaatataa aaattgaagt
     3301 ctaaaaataa cttggaggta aattaaagtg gaaaataaaa attatagact acttccctat
     3361 agcgttattt tagaggcatc aaaaggggat ataagtgcaa tagaagtagt cctaaaacac
     3421 tatgaaatgt atataacaaa gttgtgtaca agaacagtat gtgataaaca aggtagaacc
     3481 tttatttatt tggatgaaaa caagaagagt aggatagagt taaaacttat aaccaaaatt
     3541 ttaactttca agccttaatt aaaaaatagt attatgagat aaagataatt tcaaaaataa
     3601 aaatttaata ggaaagaata caattaaaaa gttattacac aaggaataga cattgaatgc
     3661 aaatttcaat gtctattctt tatataaaaa aatagatttt aaataaaaaa ataatattaa
     3721 gttttcaatg tttatatagt atattattac cttgtagatt taaaaaatgc attttagtac
     3781 ttctttgatt ttgtcatttt tttatttttt taacacaaat tttggtgaaa aaatagaatt
     3841 ttgaaaatat ataataatac ttgaaagtaa tatgtgtaat ccgtatgtaa ggaaatttat
     3901 tagaaaaggg tgaaattata tgactagatc aaggaaaaac ttaaaaaata acagaaaaag
     3961 gttaattaaa aataatttaa ctaaaacaat aacaacaact gtaatagcaa ctggagtatg
     4021 tgctcctttt agtttgacaa atgtttatgc agaagagata aaatcagttg ataaacaaat
     4081 tgaagcaaaa aaaatagatg aaaaatatcc tgaaacaggt attacaggtt ggggtttaga
     4141 agatgaaaat ggaaaagtaa tagaaggtca tggatcagaa gcgaaaaaag tagataaaaa
     4201 ataccctgag acaggtatta caggttgggg tttagaagat gaaaatggaa aagtaataga
     4261 aggccatgga tcagaagcaa aaaaagtaga taaaaaatac ccagaaacag gtattacagg
     4321 ctggggctta gaagatgaaa atggaaaagt agtagaaggg catggatcag aagcaaagaa
     4381 aacagataaa tttagtgaac aattaaaagt taacactgaa ggtgaaaaag ataagattaa
     4441 aactcaaaat gaatcgaata aggaatatat atttgaaatt tttgatttga aaaaattaca
     4501 agagattaaa gataaattag ataaatataa aggcaaatta aataaagaat taagcatagg
     4561 tttagaaaga caaaagagca atattgagat atatattggt ttatttaaag cgaataatga
     4621 ttgctcaaat tttaaagatt tagttaaaaa accagatgtc gatgaaaatg cctattatag
     4681 aattttaaat tttattaatg aagaatatga taagtttaaa gaggcatgtg acaaatacga
     4741 tagagaacaa acaggtaaga aatcagaaca aggtaagcag cctgaagata agaaaccaga
     4801 tcatagtgaa ctagaagaaa gtgaaatagc aattgagaaa cctttaaagg atataaaaga
     4861 taaggcactg agtgataatg ctagattaca tagtgaagta gattcactac tggataagta
     4921 tggaagtgtt ttggggctag ataaaagtgg tatagctgct gagtttggag cgattgaaga
     4981 gattatcaaa aatgctgatt cctatcaaaa gatacttacg gttggatcta tgtcagagtg
     5041 tgtaaaaaaa gaatatgatg ttacagtagt tagtaatttt ttagaaaaag aattaaaaaa
     5101 gatagaaaaa gttataaaca taaatacgga aacagaagaa aataaagaat tattaagaga
     5161 agtaagggat atttcaaaaa aagttctaga gttaaataaa cttaaaggat ttattagaga
     5221 acttaatggg aaaaatgata cattgattgc aaaagatgaa tttgaagaaa tttataaaaa
     5281 tatacgtgaa attaaagaaa aatacaactc aatatttgat gaagggtatg tagagaaggc
     5341 tagattactt tatatgaaac taaaaaatgg taatttagaa aaagatcaga gctctattgg
     5401 gaaagaacgt gcagaagtta tggacaagat aagaaaatca aagaacaaag aagaattagt
     5461 agatatactt aaaaagccta taccaggtaa cattgacgag tttgataaac agtatatgga
     5521 gagcatttta ttgaggacaa caaatgacga gctagaatct cagaagaata aattacttga
     5581 tttattatca agagaaagag gaaacttaag gtgggaaggt attaaggaag aagataagca
     5641 gcctgaagta aa
//
LOCUS       DAGSWI010000188         5624 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.192, whole genome shotgun sequence.
ACCESSION   DAGSWI010000188 DAGSWI010000000
VERSION     DAGSWI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.192"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(70..2481)
                     /locus_tag="MJF80_003596"
     CDS             complement(70..2481)
                     /locus_tag="MJF80_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBZ0282205.1"
                     /translation="MRKYKSKKLSKLLALLTVYFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGEALT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGNDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            2709..3107
                     /locus_tag="MJF80_003597"
     CDS             2709..3107
                     /locus_tag="MJF80_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBZ0282206.1"
                     /translation="MIFKKHKETILYLFFGTLTTFVNIISYLFFTRVVSLNFIIANIL
                     AWILAVLFAYFTNKFFVFESKVIEIAFLFKEFLSFVSFRLFSGITEMLIMYIMIDLLF
                     INDIIVKVFTNIIVIVLNYVFSKMIIFKKL"
     gene            complement(3168..3875)
                     /locus_tag="MJF80_003598"
     CDS             complement(3168..3875)
                     /locus_tag="MJF80_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBZ0282207.1"
                     /translation="MIEATLFFSPHQDDEILSMGSAIIEHVEKSDTHVILCTDGSKSV
                     IRKVLDDGGACSYHIKDIHKYSLSEDEFSYNRDDEFKNSCKEMGVKESNIHIEENRAH
                     DGELNKEKAKEIILKYLEEYPNAKVKTVTPYKASGIHEDHRALGEAALELYKEGKIKD
                     LRFYVEPYDYNDFKKVNPNVKVWKLANSQENKLLNAMNAYKKWNPESGKYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            complement(3946..4383)
                     /locus_tag="MJF80_003599"
     CDS             complement(3946..4383)
                     /locus_tag="MJF80_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBZ0282208.1"
                     /translation="MEYVNLIFLSMIPIVELRGAIPMGLALNLNPVLVYISCIIGSTV
                     IGIPILLTFRYMLDYLKVKGYFKCFVDFVENKLHNRAKKLKSLSIVGIVLFVGVPLPT
                     TGTWSAACIASLLRMRLKDAILGIFLGNIVSGCVVFAISYHIF"
     gene            complement(4456..>5624)
                     /locus_tag="MJF80_003600"
     CDS             complement(4456..>5624)
                     /locus_tag="MJF80_003600"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBZ0282209.1"
                     /translation="YKEIKEIINANIIGALILTLILFIIKLINYSRIVLMLFVMYNVI
                     LTSLSRVVLRSILRKYRSKGFNQKHCLVVGATETGKKLIGKIKKNKQWGYNIIGCLDD
                     DLYHLDRFEEILILGTIDEIETILEKTHVDIVFITIDSEKFYKFGEIIKQCEKAGVKT
                     NIVPYYYNYVPSKPYMDDLDGFPIIDTRHVPLDNYFNNACKRLFDIIFSIIGIIITLP
                     IMLFSILMIKLTSSGKIIYKQTRVGLNRKNFDMYKFRSMKTQKEEEEITKWTTKNDSR
                     KTKWGSIMRKTSIDELPQFFNVIKGDMSIVGPRPERPYFVDKFKYEIPRYMIKHQVRP
                     GITGWAQVNGYRGDTSIEKRIEYDLYYIENWTFLFDVKIVFFTIFKGILNKNAY"
BASE COUNT         1713 a          931 c          656 g         2324 t
ORIGIN      
        1 ttatatttat aaacttctat tgaatatttt ttaataataa aatgtatatc cttactatct
       61 tttcattcac tattttccta aaagagtatt tagttcatta aaagcacttt catttccatt
      121 tccaccaact ctaataggag tttctatttt cttagactta agtacatttt tttgactttc
      181 atctaactta tttccaacta gaactactgg cgaatcagtt ttagcaccta atgctcctac
      241 tgctaatcca tctatcaagt ctccttgact ctttgaacca tctttaacaa caaaagcata
      301 tttcaaatct gaactagggt agaatttatc tattatctta gcattagttt catttctatt
      361 acttcctgca agtcttgttg gatttgaaag cttactttct aaatttgatg ataaagtagc
      421 tgttccacca attatataag acttagttat ttttgatgaa tttaaaaact catcagcacc
      481 ttgcaactca ctcttttcat tagtaagtat tattggcata ttgttatcag cagctgctgc
      541 accaacactt attgcatctg caagtccatt tacaccattt gccactgcaa cttgtgagac
      601 agattttata ctatttaact tttgagctag agatatacta gtcttgtatc tatcattacc
      661 tgaaattctt tctaccgata tacccatatt gctaagttga gtttgtacat tcttacttaa
      721 agaattttct ccacctatta gataaacttt tttagttcct aatctttgta actccgcctt
      781 agtttttgaa tctaagttat cttttcctgt taaaagaatt ggagagtcga ttgctgctgt
      841 aaatggtgta gctgttaaag catccactat tgcgtctcca tttactaata ctgcattttg
      901 tgaagaagtc catccttttt ggctaacttt aactgctgtt tcatatctat ctgctccagt
      961 taaagcttct cctgaagtag cttgtccaga agatgttttt gttatagctt ttataacaaa
     1021 gttacaagga gaaaattcac tatatgtatt tatatcttca aacttattgt tttgaagtat
     1081 gtagctttca cctaagtttg ctttagcctc atatagatag tatccatcta tattagtttc
     1141 atatgctaag gttgattttt ctctattagg atttgctgtg ttgtctataa ctactactat
     1201 tgctccttta ccttttggta aactgtaaga attagtaggt acatttatgt atccagaata
     1261 tgatactgtt ccacttgcaa gttttgtcat tgaattgttt tgaggaactc catttactac
     1321 tggtgcataa tatacttcat attttgctcc tacagagtct gtttcaaaca taacagaatc
     1381 aagtttttca gaatctctgc taaaatcaaa tacatttgct gctgttactt tatttgacat
     1441 tatcttacta agaccagcat attcaagttg gtacattttt ttatcactat ctggttttga
     1501 tactgatttc attgcgtagt tatctgtatc tgttagtaga gtcttatctt cataagaaat
     1561 ccagaagaat cctttcatag aattaaactc tccccaactg ctttttacta accatgctcc
     1621 atttgattct ggttttacat cagatgcaaa gttatctttt gaataattat catcccatcc
     1681 tactatcgct acagcgtgat ttaatggagc tctcttattt gtacagttat atgctgtttc
     1741 atctttatta aaatatgttg aataatgtgc ataacctgat gtcactgaac catattgcat
     1801 tatagcattt tttacagttt ccttatcttt attaagacga acaacatctg ttacattaaa
     1861 ttgagtaggt gcagtatcca tatttgaagg tttagttgca ccttgtgcct cagatttaag
     1921 attatatggt atatcttttt ctaacttagg acctgcccat gctgtcagat atcctatggc
     1981 tgttacattt gaactacctg acatatcatc taagttccat ccatattttc caccagttgc
     2041 ccaccatctt aaatgttctt ctgataagtc atacgttcca tatcctttta atttaagata
     2101 tgcttctaaa gttgacatac ctgaaaaaga ccagcatgta ttaagacttc cttgattttt
     2161 tgctggtgta gtaagaccaa gattcattgg gttgtatgct acacttgatt tatatgctga
     2221 tggaagacta cttctaccct tatttcgaag tgttgtacct tccatttcat gtggaaaagg
     2281 tattactccg ttatattttg ctgtgtctcc atttttgaca tcttctaggt attgaagata
     2341 gctttcagaa tactctgcag tttctactcc atctagagtt ttatggtttt ctgctgagac
     2401 aggtattgtt gacactatta aaaagtaaac tgtcaacaat gctagtagct ttgacaattt
     2461 ttttgatttg tactttctca tgtttacccc cttatatgta tttaattttt attatatcag
     2521 atttctaaat ctaggtcata ttttattttg ataaaataaa aattgtaagt aaatattaat
     2581 tgtaaaatat tataatttta ttttttatga tattttacaa tatgtctatt tgttaactga
     2641 tatgctatac taaagtgtat tagtgttttt aaagatagtt atatttgatt agtttaggag
     2701 ggttatattt gatttttaaa aaacataaag agaccatatt atatttattt tttggaacac
     2761 ttactacttt tgtaaatata atttcatatt tattttttac tagagttgta tcacttaatt
     2821 ttataattgc gaatatttta gcatggattc ttgctgtctt atttgcctat tttactaata
     2881 agttttttgt atttgaaagt aaggtaattg agatagcttt cttatttaaa gaattcttat
     2941 cttttgttag ttttagatta ttttctggta ttactgaaat gttaattatg tatataatga
     3001 ttgatttact tttcataaat gatattatag taaaagtctt tactaacata attgttatag
     3061 ttttgaatta tgtatttagt aaaatgataa tttttaagaa actataataa aaaatagtct
     3121 gctgaagtag cagactattt tttattatat atgaggcgaa ttgtaagtta aggagcatgt
     3181 acatattgaa tcttatttga ctttaactca tcaaaatgtg attttacaga atgatatcct
     3241 attgcatatt ttccactttc tggattccat tttttataag cattcatagc atttagtagt
     3301 ttattttctt gactatttgc taacttccag acctttacat ttggattaac ttttttaaag
     3361 tcattataat catatggctc tacataaaat cttaaatcct ttatttttcc ttctttatat
     3421 aactctaatg ctgcctcacc tagtgctcta tggtcttcat gtattccact tgctttatat
     3481 ggagtgactg tttttacttt tgcatttgga tattcctcaa gatattttaa tattatctct
     3541 tttgcttttt ctttgttaag ttctccatca tgagctctat tttcttctat atgtatatta
     3601 ctttctttga cacccatttc tttacagctg tttttaaact catcatccct attatacgaa
     3661 aattcatctt ctgataaaga atacttatgt atatctttta tatgatatga acatgcccct
     3721 ccatcatcca aaactttcct tatgactgat ttacttccat ccgtacataa tataacatgc
     3781 gtatcacttt tttcaacatg ttcgattatt gcacttccca tacttaaaat ttcatcatct
     3841 tgatgtggtg aaaaaaataa agttgcctct atcaattacg ttcctcctat tctataaata
     3901 aaaataaatg tttatttaaa agctttacta ttctactaaa tgtacttaaa atatatgata
     3961 agatatagca aaaactacac atcctgaaac tatatttcca agaaatatac ctaaaattgc
     4021 atctttcaat ctcattctaa gtaaagaagc tatgcatgca gcactccatg ttccagtggt
     4081 tggtaatggg actccaacaa ataaaacaat acctacaata cttagcgact tcaatttttt
     4141 tgctctatta tgtagtttat tttctacaaa atctacaaaa catttaaagt acccttttac
     4201 ctttaaataa tccaacatat atctaaatgt taataatatt ggtataccta ttactgttga
     4261 accaattata caggagatat aaactaatac aggatttaag tttagtgcaa gacccatagg
     4321 aattgcccct ctaagctcta caattggtat catggataaa aatattaaat ttacatattc
     4381 caatatattt ctccttatta taagtaattt aattctttaa ttgagatttg ttttagttat
     4441 aaaataaaat ctatctcaat atgcattttt atttaatatc cctttaaata tagtaaaaaa
     4501 aactattttt acatcaaata aaaatgtcca gttctctatg taatataagt catattctat
     4561 tcttttttct attgatgtat ctcctctata tccatttact tgagcccaac ctgttattcc
     4621 gggtcttact tgatgtttta tcatatatct aggtatttca tacttaaact tgtctacaaa
     4681 atatggcctt tctggcctag gacctactat tgacatatct cctttaatga cattaaaaaa
     4741 ttgtggtaac tcatctatac ttgtttttct cattatcgaa ccccatttgg tctttctaga
     4801 atcatttttt gttgtccact tagtaatctc ttcttcttct ttctgtgttt tcatagatct
     4861 aaatttatac atatcaaaat tttttctatt taacccaact cttgtttgct tatatattat
     4921 tttccctgaa gaagtcaatt ttatcattaa tattgaaaac aacataattg gcaatgttat
     4981 aataatgcca attattgaaa aaattatatc aaatagtctt ttacatgcat tattaaaata
     5041 attatctaat ggtacatgtc tagtatctat tattggaaat ccatctaaat catccatata
     5101 aggttttgat ggtacataat tataataata tggaactata tttgttttta cacctgcttt
     5161 ttcacattgt tttattattt ctccaaattt ataaaatttt tctgaatcaa tagttataaa
     5221 tactatatcc acatgtgttt tttccaatat agtttctatt tcatctatag ttcctaaaat
     5281 taatatttct tcaaatctat ctaaatgata aagatcatca tctaaacatc ctataatatt
     5341 atatccccac tgtttatttt tctttatttt tccaattaat tttttaccag tctctgtagc
     5401 tcctactact aaacaatgtt tttgattgaa ccctttactt ctatactttc tcaatatact
     5461 ccttaacact accctactta aactagttaa tattacatta tacattacaa ataacataag
     5521 aacaattctt gaataattta ttaattttat tatgaataga attaatgtca atattaatgc
     5581 acctataata ttagcattaa ttatttcttt tatttcttta tata
//
LOCUS       DAGSWI010000189         5445 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.193, whole genome shotgun sequence.
ACCESSION   DAGSWI010000189 DAGSWI010000000
VERSION     DAGSWI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.193"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(204..1388)
                     /locus_tag="MJF80_003601"
     CDS             complement(204..1388)
                     /locus_tag="MJF80_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBZ0282210.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1761..4112
                     /locus_tag="MJF80_003602"
     CDS             1761..4112
                     /locus_tag="MJF80_003602"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBZ0282211.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDNLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4134..4673
                     /gene="nrdG"
                     /locus_tag="MJF80_003603"
     CDS             4134..4673
                     /gene="nrdG"
                     /locus_tag="MJF80_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBZ0282212.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2136 a          613 c          866 g         1830 t
ORIGIN      
        1 ttataataaa tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata
       61 aaagacttac tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa
      121 gttgttgaat atatcttata ttacttacct tatatattaa ttaccattaa tacattatat
      181 ctaattacta ttcatctaaa ttactatgcc attattttat atacaaattc ttttaattta
      241 tctaatccag ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc
      301 ataccaaatg ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa
      361 aattctatag aaaaactatc tttatattca aatttctcag atactttaga aagatctata
      421 aatatataga atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt
      481 gatttaatta aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag
      541 caatctttca aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga
      601 tgtccttgta tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat
      661 cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta
      721 gcttcttcgc ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat
      781 atttcatctg ctaaaatata tattttattt tgtatgcata catctactat ttctattaat
      841 tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac aagaattttt
      901 gttttatctg ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt
      961 ttagtatcaa taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc
     1021 caataaggct ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt
     1081 gttattgagt tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta
     1141 ttttcttcaa taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa
     1201 tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt
     1261 acattaaaat cgggttctcc tatactaaga tttattactt taactccatt actttccatt
     1321 tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt
     1381 gataacataa aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat
     1441 ttacataagg aaatttgtag atatatacat taatatatat aatagcctac actttttgcc
     1501 taaaattagt gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgctg
     1561 ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca
     1621 aatttggtaa tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga
     1681 tttttttaga cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata
     1741 gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc
     1801 catttaatga agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa
     1861 gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt
     1921 ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag
     1981 ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa
     2041 ctgaaagaag tagaataaga aattcaaaaa caaggttgat gaaggcaata gaggaaatta
     2101 cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata
     2161 cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac
     2221 atatattaaa accagagcat tcttttgctc atgataatgg agatatacac atacatgata
     2281 tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca
     2341 atggtggatt ttcaacagga catggctttt taagagagcc acaagatata ataagttatg
     2401 gtgcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta
     2461 taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca
     2521 taggaaattt agctaaagca ttgaagttat ttaaaggaat tgaaaataat gatgtgataa
     2581 agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg
     2641 aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac
     2701 ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa
     2761 catatcaatc tatggaagca tttatacaca atctaaacac aatgcactct agagcaggtg
     2821 cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg
     2881 taactaagaa tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt
     2941 ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt
     3001 atgatttatt taaattatca tgcagagtat cagctaaaag actattccct aattttagct
     3061 ttttagatgc gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaggcta
     3121 catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg
     3181 gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata aaacatggta
     3241 ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat gagaagataa
     3301 acttaattat agaacaatta ttggagcgcc ttgaggttca aggaaataaa aagatgaaga
     3361 attttccatt cttaatgggg caaggtgtat ggaaaggttc tgataattta ggaccagaag
     3421 atacactaaa agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag
     3481 agtgccttat tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag
     3541 gattaaaaat agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat
     3601 taaatttctc cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag
     3661 ataaaaaagt ttatggagaa attaaaggta tcacagataa agaatattat actaattcat
     3721 tccatgtacc agtttattac aatataagtg catatgataa aattgagata gaagctccat
     3781 accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg
     3841 ataatttaga agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg
     3901 gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa
     3961 gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa ttcgaaagaa
     4021 taagaagaat aacaggatac ttagttggta cagttgatag atttaataat gctaaaaaag
     4081 cagaagtaag agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa
     4141 taagaatgtc atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg
     4201 taatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg
     4261 atttatgtgg aggattttat atggatacag aagaaattat aaataaagtc aaatcattga
     4321 aattacaaaa aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag
     4381 aagaaatagc aagagaagct aaaataaatg ggttagatgt atggtcatat actggattta
     4441 cgtttgaaca actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa
     4501 agcaaattga tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa
     4561 agtttagagg gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaaa
     4621 aagtgttttt ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa
     4681 ataaacagga ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa
     4741 gcttgtataa tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt
     4801 atagtttata ggtgttttat gtggattacg aatgatatag tatcgataca ttatttaata
     4861 gattcttatg ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat
     4921 ctagataagt tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt
     4981 gtgaatatta agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac
     5041 taaacacata agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat
     5101 gtaatatcta tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat
     5161 aatgtggata atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg
     5221 gataatatta taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt
     5281 tgttgactct aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca
     5341 aataaagcac aaagaacttt gaaaattaaa cagtaggtta atttataaaa caagaaacaa
     5401 accataaagc cagatatttt gataacaata gtatctgagc ctgat
//
LOCUS       DAGSWI010000190         5429 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.194, whole genome shotgun sequence.
ACCESSION   DAGSWI010000190 DAGSWI010000000
VERSION     DAGSWI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.194"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            60..1241
                     /locus_tag="MJF80_003604"
     CDS             60..1241
                     /locus_tag="MJF80_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBZ0282213.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1383..2300)
                     /locus_tag="MJF80_003605"
     CDS             complement(1383..2300)
                     /locus_tag="MJF80_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282214.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2553..3347
                     /locus_tag="MJF80_003606"
     CDS             2553..3347
                     /locus_tag="MJF80_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282215.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3348..5408
                     /locus_tag="MJF80_003607"
     CDS             3348..5408
                     /locus_tag="MJF80_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBZ0282216.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2072 a          622 c          902 g         1833 t
ORIGIN      
        1 agaatattag aaataataaa taaataatac attgtactta aaagggggag aaatttaaga
       61 tgaacaaaaa atatattaaa gcagggaatt taattgatgg aaccggaaat agtattatgc
      121 aaaataaggg tataattatt gatggagata ctatagttga aattgaagat ataagagatg
      181 atttaaatgg atatgatgtg tacgattact tagataaaac tgttatgcca ggcattatga
      241 attgtcatgt tcacttgaca atggagccag ttggaaatcc aaagatatat tatgataatg
      301 tatcagatgt agagcttgta gtaaatacga taaaacaact tgatgcttat ttggattcag
      361 gagtaacata tattagaagc ttaggttgtc ccaaatatgt ggatgtacaa cttaaaaatc
      421 ttatagacaa agggtctata gatggtcctg gtatagttac ttcaggtcca gttatatgta
      481 tgacaggagg gcatggatat tattttggta ttgaaagtga tggagtagat gaatgtagaa
      541 aatcagccag aacagtttta aaaaatggag tagattgtgt aaagataatg gcaactggag
      601 gtgtaacaac tgatggagtt gagccagggt caccacaatt aactttggag gaaatgaagg
      661 ctgctgtaga tgaagctata aaagcaggta aaataactgc cacacatgca caaggaagaa
      721 ctggtataaa aaatgcagtg cttgcaggaa ttacttctat agaacatggt gtttatttag
      781 atgatgaaat aattgatttg atgcttgaaa gaggaactta cttagttcca actgtggctg
      841 caccatattt tattctaaat gcaggaaaag aaagtggaat aactgaagct actcttagaa
      901 agtgtgagat tgttgccatt ccacacaaag agtcattcct aaaagcatat aaaaaaggaa
      961 ttaaaattgc agttggttct gatgcaggaa cttcttataa tgaacatgga aaaacatatt
     1021 atgaaatgaa actaatgtca gattatggta tggataatat ggatataata gttgctgcta
     1081 caaaaacagc atctgaactt ttaagaattg ataaaaatta tggaactcta gaaaaaggaa
     1141 agaaagcaga tgttatagtt gtaaatggaa atccagtaga gaatatagac gtacttgcag
     1201 atgtgttagc agtatttaaa cttggtaaaa aagttagata aatagtataa aaataaaaaa
     1261 gagtgtctaa agaaattttt aaataattat agacactctt ttttattagt aataattaaa
     1321 tcttttatac tttagaaaat agattctatt ttatatttag atataactct ttaaaatcat
     1381 tcttatgcaa tataatctat agaatctaca catacaaagt atattcttga atcattagaa
     1441 tttccaagta ctataacatc atttttctta cctagaactt caacaccagt tagaaccaaa
     1501 ctaccagcta ctacagtaac ttgattattt atatttgaca tataaagatt atctaggata
     1561 cctttattac aacagcaatc atcacaatcg cagttgcaat tacattcttt gtaatgtttt
     1621 ttatcaagat agtaacctaa agatgctatg aaatcttcat aatctgcagg tgtaatatca
     1681 aatgcaacag ctttaagatt acacaatgat gctaaatcta cgtttgctaa tggagtagat
     1741 gcagcaagta taaaatcaat tatagcagca agtattgcat ctaatatagc cccaaggtct
     1801 atcgtaggag gaataactgc atcaataagt gcaattaatt cagctactcc tggtatagtt
     1861 cctatagtat taattaatcc ctcaagagtt aaagggactg gaataggata agatactcta
     1921 ccagctatat ctattaaatc acagttacaa gcagaaaatc tttcaaaagt accatcaagt
     1981 ccagataaat tatcttttgg aggagctgaa agatctatac ctaccaaatt agcacctact
     2041 ataaagaaat ctgagataaa agcaaattga ttaaagttta caaaaggtct tagagcatca
     2101 tatcttagaa gttctaatgc ttctctcata ctttttttac aacaacattg ttttatttta
     2161 ggttctttat atttatttgg ataattatag tatctatcag ctacttgata attttcatag
     2221 tatctttcat ttgtatttgg ataatcttcc tcatattctt gtgtaaatct aaagtcctct
     2281 ctacatttat tattttccac taaatacact ccttttatat tttaatttaa atgtatagat
     2341 aattaagctt atctatatag taatattata tttataatgt aaaaaaatgt tacaaaagat
     2401 attataaacg aataaaaaat aataatttgt cagataaact tgtaaatttt tatgaataat
     2461 ccattctaaa acaaaaaact gtgtataaaa aaggtttata tgttatattt atataaatat
     2521 aacatatgaa cgagaaagga gtggtttgat aaatgattga ggtagataaa ttgtgtaagt
     2581 cttttacaag ggttgtaaaa gatgataaga ataaaagttc aataagaaag ctaaagaaag
     2641 taaaaactaa aaaagaagaa ttttttgctg taaataatgt ttcatttaaa gttatggaag
     2701 gtgaaattgt tggaatatta ggtccaaatg gtgcaggaaa gactacatta cttagaatgt
     2761 taggtggcat attaacacca acatcaggaa gtataaatat ttctggatat gattattcaa
     2821 tagatagaaa ttcagcaaaa aaagaaatag gttatctctc tggaaataca aaactatatg
     2881 gaaggctttc tccgagagaa cttctaacaa cttttgcaag tctatatgag atgtcaaaag
     2941 aagatataga agaatcaatt gaaaatattg taaaaataat ggatatgagt gaatttatag
     3001 ataatagaat tgaaaatcta tcaactggtc agactcaaag aacatctata gctcgttgtt
     3061 taatacattc tccaaaagta tatatttttg atgagccaac actaggtctg gatgttttaa
     3121 gtagtgcatc tattatagat tttatgaaga atgaaaaact aaggggaaaa actgttcttt
     3181 actcaactca ttatatggaa gaagcagaaa ctttatgtga caagattttt atgattcaca
     3241 atgggaaaat aattgcatca ggaacaccac agagtttaaa agaggaatct ggagtaaata
     3301 atttgagaga tgtattcata aaacttgcca gaaaggagga catttaaatg agaagtaaga
     3361 ttgtaaaata catatttaaa aaagaaatgt tagacatatt gagagataaa aagactttat
     3421 ttatgatgat tgttgtgcca cttcttttat atcctttact tatgttatta atgattcaag
     3481 taatgaatat gtctgcaagt agtatgaata gtaaggacat taatatagct tttaataata
     3541 aacctaataa ggtgttagta tccatgattg aagatgataa tttagaaaaa gatactaaaa
     3601 atacttcaaa tgataaaggc aaaataagag tgttagatgt agataactat aaaaagtcat
     3661 tagaagaaaa taaaattgat gcttatataa agatggaaga aaaaaaatca tctataaatt
     3721 atcaaatata tataaattct tctcaagaaa attcttctaa tgcactaaaa agaattgaaa
     3781 ctgttttgga taaatataaa agatttacca tagaaaaaac tttatctgaa aaaggattag
     3841 atgtacaagc tactttagaa ccaataacat atagcactgt agatgttgca aagacggaag
     3901 aagtggcagg atacttttta ggtcagatac ttccatttat attaataata ggagttttac
     3961 taggttcaat atatccagca atagatgtta tggctggaga aaaggaaaga ggtacattag
     4021 aaacattatt tacactgcca atatcaaact tagaactggt tatgggaaaa tatatggcag
     4081 tttctcttag tgcaatagta acagcaattt taaacatttt atctatggga ctgactatgg
     4141 catttatttt agttagtgga ggtattagta gccaatttgg ctttggaaac tttaattatg
     4201 gagacttagt cttaccaatt ataactactt taatttgcat atgtcttttt tcaatggtag
     4261 tttcagcaat aagtatgtgt gtatgttcat tagcaaaaag ttttaaagat gctcaaaact
     4321 acataacacc tcttatgtta attattatga taccatcata tgtttctatg ataccaaata
     4381 tagtattaga tagagtgaca gctgtaatac ctgtagtaaa tatatcttta cttataaaaa
     4441 gtgtattatc ttttaaaagc gatttaaatt taatagcctt agttttagca tcaaatgtag
     4501 cctttgttat attggcaatc atactactgt ctaagatgtt caattctgag gaaatacttt
     4561 ttggtaatag taagagtttt tctttcttag agaaaagaag taatataaaa aaagggtcta
     4621 taccaactgt aagtgatggt gttattttat atgcagtagg attagttcta ttgatttatg
     4681 ttggttcttt agttcaaatt aaatttggta tggcaggtat tataatgaca caattcatga
     4741 taatttcttt accattatta tttgcatact atattaaagc tgattttaaa gaggtattta
     4801 gcttgacagt tcctaaatta aagcatgtgt ttgggagcat tgttctttgg attggtggat
     4861 acatacttat tatgattata acacagctaa ttttattctt atttcctcaa aatatggaag
     4921 ttgctgaaag tttaaataag gctcttttta tcaaagatag tgttttactg aatttactag
     4981 caatagcttt attaccagct atatgtgaag aaatgttttt taggggattt atattttctt
     5041 catttagtaa atcaaaagat aaaaataagt ctatcaaact cgctataata tgtagtgggg
     5101 tactatttgg tattatgcat atggatttta ttagaataat tccaacatca atattaggta
     5161 ttatatttgc atacagtgtg tataaatctg gttcaatatt tgtatcaatg ttattgcatt
     5221 ttttaaataa tagtgtagca gttgtgttaa atcattatac aactggaaat ataaccaact
     5281 tatataaatt tgttgaagtt gatttttcga acttaaatgt gcttaaattt gccttaattc
     5341 ttctaataag tcttatatta attatgttag gattaagagt tttagataga aagtctttaa
     5401 gaaattaagt ataaagttat tatatagat
//
LOCUS       DAGSWI010000191         5425 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.195, whole genome shotgun sequence.
ACCESSION   DAGSWI010000191 DAGSWI010000000
VERSION     DAGSWI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.195"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            63..980
                     /locus_tag="MJF80_003608"
     CDS             63..980
                     /locus_tag="MJF80_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBZ0282217.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGNIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(1109..2038)
                     /gene="pfkB"
                     /locus_tag="MJF80_003609"
     CDS             complement(1109..2038)
                     /gene="pfkB"
                     /locus_tag="MJF80_003609"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBZ0282218.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLVEIDKYQDLVEVKKLS"
     gene            2305..3030
                     /locus_tag="MJF80_003610"
     CDS             2305..3030
                     /locus_tag="MJF80_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0282219.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            3113..4267
                     /gene="nagA"
                     /locus_tag="MJF80_003611"
     CDS             3113..4267
                     /gene="nagA"
                     /locus_tag="MJF80_003611"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBZ0282220.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNNTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYIPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(4966..>5425)
                     /locus_tag="MJF80_003612"
     CDS             complement(4966..>5425)
                     /locus_tag="MJF80_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBZ0282221.1"
                     /translation="LKENHITFEEYDHEPILSFEKAKEIREKLQYTGVESKSLFIKDR
                     SGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPFGYEKNISL
                     VIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF"
BASE COUNT         2003 a          709 c          757 g         1956 t
ORIGIN      
        1 caaatttatg tgtctgaatt ataatcaatt ctgtaaatat aaaaacctta ggagaaagta
       61 ttatgaaaaa tataaaaatg ggaatgatag gtcttggctc catagctcaa aaggcatatt
      121 tacctatatt aactaagtct gagagatttg agtttgtagg agcttttact ccaaataaag
      181 ttaaaaggga aaaagtctgt agtgattata gaatcatgcc attcgattct attgaatccc
      241 tagctaaaaa atgtgattgt atttttctac acagctctac agaaacacac tacgaaatta
      301 taaaaatatt attaaattta ggtgttcatg tatatgtaga caaacctctt gcttcaacag
      361 taagtcaagg agaagaactt atcgaattaa gtacgaaaaa aaacttaaat ttaatggttg
      421 gattcaaccg tagattctgt cctatgtaca aagaaattaa aaataatgct acagaaattg
      481 tatctatcaa tatatgtaaa catggattaa attccttaag aaatgttaaa ttcgacagta
      541 ctcttataga tgactatata catgttattg acactgcttt atggttagca aatgaagatg
      601 ttgaaattag tggagaggat ttatttttaa cagacaataa gaatcttata tttgtaagtc
      661 ataaacttaa aggtaaaaat ttctctataa acacctctat gcatagagat tctggaacaa
      721 agttagaaca agttgaaatt ctttcaaagg gtaatattca aagagttaag aatcttaatg
      781 tactggaaat agaagaaggt ggaaacctaa ctttaaagca aagtggtgca tgggtaaaca
      841 tattaaaaca aaagggtttt gaggatatat caaatcattt tattgattgt atagaaaata
      901 atataaaacc agctattaat ggagaagagt gtatcaaggc tcaaagatta ctagagaaaa
      961 ttataaattc agtaaaatag atatttatta taaaaatagg ttaaaaatga atttttctaa
     1021 cctattttta tataagacta tatcttttat atcaaaatta aaatctatta caacaacatt
     1081 aaatttatta tattcccatt aaatgtattt aacttaactt ttttacttca actaaatctt
     1141 gatatttatc aatttctacc aaatctacaa atccagtttc catatttaaa gcattactta
     1201 taccacaagc atttgcaaat ttaagtaaat tttctatttt ataacccctt aaaataccta
     1261 cactaaatcc agccactgtg ctatctccac tacctacagt atttatacac tttacaactg
     1321 gtacttttac atcatataca ttatcttcgt ttaaatacac cataccatct tttcctaaag
     1381 atatacatac attctctgca cccatttcaa taagtttttt tcctgacaag ataatttctt
     1441 ctcttgattc tatattcatt cctaataaat gtcttatttc atctatattt ggctttatta
     1501 aatatggttt tgattttaaa gctattttta atgcttctcc tgatgtatct aatataaact
     1561 ttatattatt tgctctacat atatttccta tcttttcata atagtctatc ggcatattct
     1621 cacaataact accagaagct accagtattt tagtattttt tagtacttcc tctatatcat
     1681 tttcaaatct ctctaaatcg cttttttcta ctattggtcc tttttctaaa aattctgtac
     1741 taactttatt gtcctcaatt atgtttaagc aatttctagt ttcctgctct atttttgtaa
     1801 atttattttg tatatctagt tttctaagtt cattttttat aaattctcca ttaaatccgc
     1861 ctagaaatcc catagctaaa ggattttctt ttaaaatttt acatacctta gctacgttta
     1921 atcctttacc tccagcagtt gcattcttac ttactaccct ttgcacctca cctatgttta
     1981 tattattcac tctatacatt ctatctatag atgggttgaa tgttattact gtaatcatac
     2041 tagattctcc tcattattaa tttttacttt tctaaacaat aaattaatct gtaaaataaa
     2101 aaatacctca tataatatat ccaaagtggt tattaccaat ttgaatataa attattttac
     2161 ttgttttgtc aatgtaccat aattttataa taaattaata gccttactta ttgaattttg
     2221 attaaacttt attcattgac ataaataatt ctatattcta taatcaaatt gtggtaataa
     2281 ccacttagaa aggactatat ttttatgata aaaagaaatg ataaaagacc aatatatgac
     2341 cagcttgttg agatattaag aactaagatt gaaaatgaga tggaaccaaa cgatagaatg
     2401 ctttccgaaa gaaaaatttg tgatgaatat ggagtaagta gaaccacagt tagactagct
     2461 atggctgagt tagagcatat gggatatata tacaaaagac atggaaaagg aacttttgtt
     2521 gctgcactaa gtcaaaattc tcaaaactta atggaaagtt atagctttac agattatatg
     2581 agaacacaag gaaaaaagcc aggtacaaag gtattatcat ttgaggtagt agaaagtaca
     2641 aagtatttct ctgaaaaatt aggtataact cctggtgaaa aaatgataaa aataatgcgt
     2701 ttaagacttg ctgatgacct tcctatgatg cttgaaagaa catatcttcc tatgaaagaa
     2761 tttataggat tgacaaaaga aaaaatcgaa caaaaacctt tatatgaaat atttagagaa
     2821 gattatggcg agattattaa agttgctgat gaagaatttt cagctggaat aatctctgat
     2881 aaggaagcta agttattgga agtaccagga gattcacctt gtctgaaatt acttcgtacc
     2941 acatataatg atgacaatat agttattgaa tttactttga gtgtcgctag aagcgataaa
     3001 tttgtatata gagtaagaca tgtaagatag taagttaaat agctttcaag aaaatagaat
     3061 gatattataa gcgtcaatgc cacaatgata gtataattat aaggaggtta ttttgaaaag
     3121 ttttatttat gcagataaat tttttatgaa acatggaatc aaagaaaaag gttaccttag
     3181 tataattaat ggcaaattcg gtgaatttca acaagaaaga cctaataata ctgataatgt
     3241 tatagatttt tcaggtaaat atattgctcc tggcttagtt gatactcaca ttcacggtct
     3301 tttaggtgca gatgtcatgg acaatacatt tgaagcaata aacacaattt caaaaggctt
     3361 attaaaatat ggagtaacat ctttcctacc tacaacttta actgattctg tagatacact
     3421 aagtgattct gtagaaaata taagcaatag cttcaatcga gttgagggtg caaaaataca
     3481 agggatttat ttagaaggtc cattttttac agaaaaatat aagggcgctc aaaatgaaaa
     3541 atattttaaa gaccctgaca tcaatatatt aaaattatgg caaaaaaaat cgaaaaattt
     3601 aataaggaaa atagcaattg ctccagaacg taaaggttca atagaattaa caagatatgc
     3661 aactaaaaat aatatatcag tttctcttgg tcatagttca gctacatttg agcaagttaa
     3721 acaagtcgtt aattcaggtg ctaaagtgtt tgtacataca tataatggga tgagtccttt
     3781 aaatcataga gaacctggaa tggttggggc tgctatggta ctaaaagata cttatgctga
     3841 attaatttgt gatggtcatc atgtaagccc aatagctgtt aaaataatga tggatgctaa
     3901 gtctagtaat aacatagctt taattacaga ttgtatgaga gctggtgcta tggaggatgg
     3961 aaaatatact cttggtaagt ttaatgtaaa tgtaaaaaat agtgttgcaa gattaaatag
     4021 tgggagttta gctggaagtg tattaactat ggacaaggct gtgagaaata tagttgattg
     4081 gaacatatca actttagaag atgcaataaa aatggctaca tatatccctg ctttaagttg
     4141 taatattgat aatgtctgtg gctctatata tcaaggtatg gatgcagact ttatagtaac
     4201 agataatgac tttaatatac acgaaactta tataaatggt atctgtaagt acaaagttaa
     4261 taaataggtt caatataagg ttatggaggc tgtctaaaaa gagtctggaa atatttaaag
     4321 ttatttctcc aaaatctgat ttaacacttt tatttccata tcgttaataa attattgcga
     4381 tagaggttat tttaaacaaa tatatattat aagtattttt gtgataatat tttcgataat
     4441 atgtacattt aaagtaactt ttctattatt atttgtaagt ttttatcttg agcaaacata
     4501 taaattttta ataaagcatg agataaaccc tatatttcaa ttactaatga tgagattttg
     4561 attgataaca gatatatttt caaatgtaaa aatactaaaa atcatatcaa ttttaaagat
     4621 attgtaggtg taaataaaca agataatact atagaaattt ttacaaatga aagcacttat
     4681 tgtatatatc taaaagttct aagcattgag agcgaagaat atctaataga tagactaaaa
     4741 tcattttgtt gattttatat attttatgtt aaatataaag cagagtagtt aaaactaccc
     4801 tgcttttgta attaaaattt aaaaataata actcataata cattgaattt gagcttaaca
     4861 gtaactattg taataatagc atcaatcttt tgtgttgaat ataatttatt tacactatag
     4921 ttaagcatga tttctaatgc tattttctct aatttacaat tatgattaaa attcattaat
     4981 atatatatat ttataagttg tattgtttat attgtttatt acttttttaa atccactagc
     5041 tgtaataata aatgtctttt ctggtatccc tggtgaaaag attattttat catggttaaa
     5101 tatatcttga tctattacaa gagagatatt tttctcatat ccaaaaggag atacacatcc
     5161 aggcaaacat tgtgtttttt gtgttaattc ttcttttgaa catatagata cttttccacc
     5221 taaaacattt tttatagttt ttggatctaa tttttttcct tctgtggtta caaatatata
     5281 gtaatttcca gacctatctt ttataaataa acttttactt tctactccag tatattgtaa
     5341 tttttctctt atttctttag ctttttcaaa tgataaaata ggttcatggt catattcttc
     5401 aaacgttata tgattttctt ttaaa
//
LOCUS       DAGSWI010000192         5342 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.196, whole genome shotgun sequence.
ACCESSION   DAGSWI010000192 DAGSWI010000000
VERSION     DAGSWI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.196"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(324..656)
                     /locus_tag="MJF80_003613"
     CDS             complement(324..656)
                     /locus_tag="MJF80_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBZ0282222.1"
                     /translation="MNTREMKEYRVFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(900..1541)
                     /locus_tag="MJF80_003614"
     CDS             complement(900..1541)
                     /locus_tag="MJF80_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBZ0282223.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLIYKISGKNN"
     gene            complement(1522..1815)
                     /locus_tag="MJF80_003615"
     CDS             complement(1522..1815)
                     /locus_tag="MJF80_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBZ0282224.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            2208..2267
                     /locus_tag="MJF80_003616"
     CDS             2208..2267
                     /locus_tag="MJF80_003616"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBZ0282225.1"
                     /translation="MTEYIYYYNNKRIKKINLA"
     gene            complement(2783..3535)
                     /locus_tag="MJF80_003617"
     CDS             complement(2783..3535)
                     /locus_tag="MJF80_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBZ0282226.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(3594..5249)
                     /locus_tag="MJF80_003618"
     CDS             complement(3594..5249)
                     /locus_tag="MJF80_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0282227.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIYIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
BASE COUNT         1941 a          836 c          456 g         2109 t
ORIGIN      
        1 ctatctgaaa aagttatcta ttaaaactgc tatgaattta taaagaatat tctagttcat
       61 aaaaatacaa atatgtaaat ataatcacct taatattaat ttatttaaaa taaaaaatgt
      121 ataaaagatt taataatgac aatatactat tctcaataca atctatgatg taaataattt
      181 aaaactgcta tattatttaa aaatttatat ttatatatgc aattctaaaa acttaataag
      241 aagtgaactt agaaaataaa gcttaatttc aaagacttaa atagactatt attattagtc
      301 taaataaact acgtctatca aaattatata ttaaattctt ctaaaatttt tattgcaaat
      361 cttgcgtttg gatatgtaat agacccgcca ccaattacac ctatttttat agcttctata
      421 atttcatctt tacttgctcc agaattaata caaccctcaa tatgataaca aatacactca
      481 tcacatcggc ttaaaattga aatagctagt cccattaatt ccttattttt tttacttata
      541 gccccttctg aataagtctt atcatcaaga cttccaaata ctctaaaaaa ctcatctgtt
      601 tcgcttaatt ttttattgta aaatacccta tattctttca tttctctagt attcatcttc
      661 tacctcctat gtttttctta taagaatatc acaccgtact tatattttca atattaaata
      721 caaattttca ttttatgttc attagcctaa tttatattat tttttgaaat ctcaataagt
      781 tatcatataa gtaagatttt ttcagaacta aaatagaaat cttagtaatt tctttattat
      841 ctatctcagt tctgaaatat attattctta tccaaacatt attatagtga ttaattatat
      901 tagttatttt tacctgaaat tttatatatc aaataagaat atataaatgg tagtattaca
      961 attacaaaga ttacaaagat attataaaaa ggaatattaa taaatgcatt aataattaat
     1021 aatattccac caataagcca aatccatcca gctaaatggt gtgtcttatt ccaattatct
     1081 tcatcattaa gagtccatgg taaaattatt cctactgagt aatttcgttt acatttagga
     1141 agatagtttc caaataaaat aactattatt cctaaaatta atcctgtata aaaattagac
     1201 tccaaactaa ctcctgttga atataaaacc atcattattt gtataattac tgaaagtata
     1261 gcaactcccc attttgataa attttgtata acatttgaaa aattagcttt cttaggctca
     1321 ttctcaagtc taaaccatgt atacaaatgg atacatagca ttaacaatgg tacaccaaaa
     1381 gctgctataa cctttggagc ataattatct ggatttccat aattatcaaa atgaattgca
     1441 acttcatttg gtaatctatt ataaaaatat attgatacaa taaatggtat aaaacatatt
     1501 aaagtagata aaataaattg tttatttatc gcttttttca ttatcttttt ctcccttctc
     1561 cataaattgt gaaaaccaaa gcatgacctc ttcgaaaact gaaatattta tttcgtagaa
     1621 aataaagttc ttatactttg tttcaaatat gagtcctgcc ttttttagtt gagatagatg
     1681 ataagaaatt gtagcattcg tcatattaaa tttttcacta atttctcctg ctgacatttt
     1741 attctccctc aacataacca atatttctct tcttataggg tctgaaagag ctttgaatgt
     1801 ttctggaaac cccaacacca tcacctcgct atttagaatt tcatctaaat agaatataac
     1861 ataacatcca ttttatttca atagctattt agaaattttt ctaaatagtt atttctaaat
     1921 atttattgaa aatttttata ttaagattct caatttactt aagtcgcttt tatttaatct
     1981 tactttatct aattttttta acttaacaaa agtgtattgc accataattg ttaaagcaaa
     2041 aacctaatga ttgaggtact tatatatata acaatacttt taattttaaa ttaaaagtag
     2101 ctaaacccaa tttaaaaagt agttaagtcc tatttaaata gaaaagaatg aaacgagaaa
     2161 tatactgtat aaaagtacat aaaaacatct actaattaga tacaactata acagaatata
     2221 tttattatta taataacaaa agaataaaaa aaattaattt agcataacct tgttaaatat
     2281 agatttgaat atgaatttaa agttatgtaa aattaaaaat acaggaatga aaattccatt
     2341 cctgtataaa atctaacttt tgagtctcag tacaatctta ttttgagaca atcttttttg
     2401 ttttatattt tcttatattt aaaatataac gcatatctta tgcaacatat tcatattttt
     2461 ctttatattc tttcatcgca tcattccaag cgtcttgtac tgttacacct cttttattag
     2521 caattttttg tgccagttca attatcttca tagctcttct tgatatgttc ataaacattc
     2581 ctcctacaaa ttaccatata tttcatatat ataaatcaag gttatatttt tatgatatat
     2641 aatccttcta ttgtaagatt acagtttata tgtgtattat atatgaacta tattagaaga
     2701 taattagaat ttttataaag ctatatgatt agattaaata taaaagcttt acaattactt
     2761 ataaatttaa aaagtctata agctaaacta atgtttgata taccaatttt acgaccataa
     2821 ataatgccat tactaaaaat attggtttta tcatctttga acctttcttt atagcaactt
     2881 tagcacctac ataagctccc attatcatca ctaatgcaaa cactatagca attttataat
     2941 ctacttgacc atttatcatg aataataaca atgaagttat attacttgtt aagtttaata
     3001 tttttgtatt tgcagaagca tgtaaaaaat catatccata tatttttata aatccaaaag
     3061 tcaaaaaagt tcctgtacct ggaccaaaaa aaccatcata aaaacccatt ataagagcca
     3121 taagcatccc tagtcttaaa tttttcttat taacagcttc aaaattatct tcttgaccta
     3181 aatttttaga aataaaggtg taaatagcca ctattaaaat cataaccaca accaaaacat
     3241 ttaatatctc ttcacttatt gataatacac atctcacacc tagaacacta ccaataatag
     3301 taaatggtac taattttttt aaaaggttgt tattaatttt tccagacttt gcatatctat
     3361 aggcactact tatacatcct gctgatgctg caaatttatt tgtaccaatg gcaagatgta
     3421 ctggcactcc aattgccata agaacaggca tgcttataag tcctcctcct cctgctatag
     3481 aatctacaaa agcagctaaa aaccccccta tacataaaaa aataactgaa aacataaaat
     3541 tcctccatca ttctctttca atttttctta caatatatat tatataatct tatttaatac
     3601 ttattatcca gcatattctt tctatattca tttggagtta tactattaaa cttcttaaat
     3661 atttgtgaaa aataactact gttattaaaa cctactttct tacaaatatc actaatactc
     3721 atgcttctat ctaacagata ttttttagat tcttctatcc taagaagatt tatatactca
     3781 gttattgaaa ctttatttct ttcattaaaa atagaactca aataatttgg acttataaaa
     3841 aaaacatctg atatactatt caaatttata ttttctttat aatatttttt tatatattcg
     3901 tttatatttt caattagatt tcctttatct tttctccaaa atgctctatt cttatcaata
     3961 cactctttgg aaaattcaaa taatacattc cataaatctt catcactgtt tacaagtgat
     4021 atttttttat tatatttatt tcttaaataa gatataagtt ttaaatcctc taaatctcta
     4081 agcactgttc tagacaatac aaatatcatt tcctgaatag tatctctaac atcattatat
     4141 cttaatttgt ttgtatctat tagattataa acaccttcta aattcatatt tttaatttct
     4201 ttttcaatat aaagttctaa ttcaaaggga tattcttttg aagaattatt gaacatatct
     4261 atcggattaa taatatactc ttcttctaat aatctactat ttgctttttt aagtgcctct
     4321 tcaagtctta tattttcttt tctactcttc aatatctcct catcttttat actaagttga
     4381 ttttcaaaaa aattcctctt agctatttcc tgaataaatt cagacatcat taataaattt
     4441 tcttttaata catataatct acttttagga tttaatggaa attcattgta aattttgttt
     4501 atgtatttta tatcaatacc agaactactt gataattcag ataaattttt atctatataa
     4561 atagacttat atactataaa attaccacat tctatatatc caactatatt ttcttctaat
     4621 actataggta tctctaaact ggaaacacca tactcacaat gatacacttt ttcaaagtta
     4681 tcattattta tgcttttaac aagtttttta gtacacggtt ttatattttt acaaatatcg
     4741 caaaatttat tttctttctt agtctcaaat atttctttat actttaagtc atatacagat
     4801 attctaagct tcaatatatt agatatttga tttgcaagat tttcaattat gtttacatta
     4861 actaactcct gtatattgtt ttcagaaaat actataggta tttgtctcaa actcgaaaat
     4921 ttatgaggaa cataagtaat taatattact aaatcaattt taccagtgtt tataatttca
     4981 tgttctgaat atggtgggca ataaactata gatgtttcct ttatattgac ctttttacca
     5041 tcaattattt gtattccttc tccctgaatc acatataaaa gctgttcttc acttaaatga
     5101 aaatgctttt cttggcagtt tcccggaaaa aatcttacaa ttccagcact aagtctattg
     5161 aattgtgttt ttgatggttc gtgtatccac ataacctctc cccaatcaaa tttttgtact
     5221 tcattatttg actcaataaa atccaccata aactcacctc gtagtttttg tctaaatatt
     5281 ttttcttccc ttcttatatt aaaataatat catcatcatt aactttcatt actataaaat
     5341 ca
//
LOCUS       DAGSWI010000193         5308 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.197, whole genome shotgun sequence.
ACCESSION   DAGSWI010000193 DAGSWI010000000
VERSION     DAGSWI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.197"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            24..1907
                     /locus_tag="MJF80_003619"
     CDS             24..1907
                     /locus_tag="MJF80_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBZ0282228.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2131..2811
                     /gene="pgmB"
                     /locus_tag="MJF80_003620"
     CDS             2131..2811
                     /gene="pgmB"
                     /locus_tag="MJF80_003620"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBZ0282229.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2808..5093
                     /locus_tag="MJF80_003621"
     CDS             2808..5093
                     /locus_tag="MJF80_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBZ0282230.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLLYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKCIKIDK"
BASE COUNT         2200 a          471 c          986 g         1651 t
ORIGIN      
        1 ttataaagga gggaaatttt aaaatgtcta ataaaaataa agaaaatata actttaagta
       61 atcaaatcaa aggaacaatt caaaatagtt taacaaaaga agctatatta catataccaa
      121 tgagcaatta tgcctatggt tatgatagag aaacactaca tataagactt agaactaaaa
      181 aaaatgaagc gaaaagagta attttacgaa taggagacca atatgtgtgg gataaaggag
      241 gtgcaggagg aggaaattta aatgcatctg gagttggttg gtctggtgga acaaatatag
      301 taatgagtaa agaagtagaa actgaattat ttgactattg gatagctaag tgtaagcctt
      361 taaataaacg ctcacgatat ggctttataa tagaaggtca agaggaaaaa cttttattta
      421 cagaaaaaag aataatagag ttgggaaatg aaaatgatga aaaggaatta tgtgaaatag
      481 gtaatttttt tggatatcca tatttaaatt atatagatat acctaaagtg ccaaattggg
      541 tgaaagatac tgtttggtat cagatatttc cagatagatt tgcaaatggt gacccgtcta
      601 taaacccaga aggagtagat aaatggggag ctattccaac cagagataat tttacaggtg
      661 gagatttaca aggtgtgata gaacacttag attatttgag tgatttggga ataaacggaa
      721 tttacttttg ccctattaca attggaaaaa ccaatcatag atatgatacg gtagattata
      781 tggaggtaga ccctacatta ggagataaag agactttaaa aaaactaata gaagaagcgc
      841 ataagagaaa tataaaaata atgttggatg ctgtttttaa tcatatagga tattattcta
      901 aacagtggca agatgtagtt caaaataagg aaaattctcg atataaaaac tggttttata
      961 taaaagatat gagtaaaatt gatactccaa tagaacaaat agatgaaaaa aatatacctt
     1021 atgaaacctt tgggtgtgaa aaatatatgc ctaaacttaa tacagaaaat tcagaagtaa
     1081 taaaatattt attggatgta ggcaagtact ggatacagga atttgatata gatgcgtgga
     1141 gattagatgt ttcaaatgaa gtagaccatg ttttttggag aaagtttaga gaagaagtca
     1201 aaaaagtaaa accagacatt tatatattgg gagaaatttg gcatggtagt ttaccatggt
     1261 taatgggtga ccaatttgat tcagttatga attacttgat gtcagaagcc atgaaaaaat
     1321 tcttttgtac agatgagata aacgccgaag aatttaagta tatgataaat gatgtaatgg
     1381 taagttatcc aatacaagtt agtgaagtta tttttaattt acttggaagt cacgatacta
     1441 caagaatatt gacttatgct aatggaaata ttgatagatt taaacttgca tatatgttta
     1501 tgtttgtaca aacaggatgt ccttgtatat actatggtga tgaaatcgga atggaaggag
     1561 aattaagttc aatatctgaa ggtcaaagaa aatgtatgga atggaatgaa gaaaaatgga
     1621 ataaggatat atttgatttt atgaagaaga ttattagact tcgtaaagaa aataaagaac
     1681 ttaggtctat atcaaatgaa tgggtgttag cagataagga aaatggaacg attatattga
     1741 aaaaagaatc tatttctata ataataaata attcatctaa aaatataatt ttgacgttac
     1801 cagattattt agcaaataaa aaagtaaaag atttatatga agaagaaata gttgatttaa
     1861 aagaagaact aatgttagag aaatacaagt tcattatttt aaaataatac aaaaaagtat
     1921 ttattttaga tttcatgtta aattattata cagtttaaac aagtctttat aaacgataaa
     1981 aaatatggtc tacaagtgag tgaattgaaa gtagatttta aattaaagac tgagttagag
     2041 tagataattt tataaataat atcttaaata caagatagaa aataaattga ttattagtct
     2101 aaaaatttct tgtagaaaga ggaaataaga atgattgaag catttatatt tgatttagat
     2161 ggtgtaatta cagatactgc atattatcac tatatggctt ggagaaaact agctcataaa
     2221 gttgggatag atatagacac aaaatttaat gaatctttaa aaggcataag tagaatggag
     2281 tcattggaca ggatattaga atttggaaat aagaaatatt cattttcaga agaagaaaaa
     2341 gtgagaatgg ctgaagaaaa aaataattac tatgtatcat tgatagatga gataacttca
     2401 aatgatattt tgccaggcat agaaagcttg ttaattgatg taaaatctaa taatataaag
     2461 ataggtctat cttctgcaag taagaatgca ataaatgtat taaatcattt gggtataagt
     2521 gacaaatttg attttatagc tgatgcagga aaatgtaaaa acaataaacc acatccagag
     2581 atatttctta tgtcagcgaa agggcttaat gtaaatccac aaaattgcat agggatagag
     2641 gatgcaagtg caggtataga cgctataaac tcagctaata tgttttctgt tggtgttgga
     2701 aattatgaaa acttaaaaaa agcaaattta gttgtagatt caacaaatca attaaagttt
     2761 gaatatatac aagaaaagta taatgagtat atagttagga gaattatatg aaatgtttat
     2821 ttagtaatgt agatgagtgg aagattatac aagataagat tgaaatcaaa gaaaatagat
     2881 tagcagagtc cataatgagt ataggcaatg gatatatggg tatgagagga aactatgagg
     2941 aaaattatag tggagataca cacaaggggt cttatatagc tggagtatgg tttccagata
     3001 aaactcgtgt tggttggtgg aaaaatgggt atccagaata ctttggtaaa attcctaatt
     3061 cagtcaacta tataggtgta agaattttca tagatgatgt agaattagat ttagcaaaat
     3121 gtaaagtaga agattttcat agagaattag atatgaaaaa tggtatttta aatcgtaggt
     3181 ttattgtaaa tataaatgat aaaaaatttg aagttagggt tactaggttt ttaagtatat
     3241 ctgttaagga acttgcagta attaaataca gtataaaatc attaaattct aatactaaaa
     3301 taaaatttgt accatattta gattcaaata taaaaaatga agattcaaac tatgaggaaa
     3361 agttttggaa tggaatttct tcaagttctt tagaatataa agggagtatt ctttctcgaa
     3421 caaaggagaa tgcatttaat acaccagtat tcactgtggg ttcaatgatg tatattaaga
     3481 caaatggtga tgtagaagct gtatctcatg agagtgatta taattactgt gaaaattcga
     3541 taacaatgga tgttttaaaa aatgaggttg caagtataga aaaatatgta tctataacct
     3601 caactagaga ttataaggaa agtgagctat taagaaaatg tgaagacatt ttaaatagag
     3661 aactatctaa aagttatgat gatatattaa atgaacatat aaagttatgg aataaaagat
     3721 gggaaagtgc agatgtaaaa ataaatggag ataaaagtag ccaacaaggt ataagatata
     3781 atttattcca actattttca acatattatg gagaagattc gcgattaaat ataggtccaa
     3841 aaggatttac tggtgaaaag tatggaggag ctacttattg ggatacagaa gcattttgtg
     3901 ttccagtata tctaggtgta gcagataagg aggtaactag aaatctttta gaatataggt
     3961 acaatcagtt agaacaggca aaggataatg caaaaaaact aggtcttaaa ggtgctttat
     4021 atccaatggt aacatttgat ggagtagaat gtcataatga atgggagata acatttgagg
     4081 aaatccaccg aaatggaagt atagtatatg caatatataa ctatacaaat tatacaggtg
     4141 actataaata tataaaagaa aaaggaatag atgtaataat agaaatagct agattttggg
     4201 caagtagagt tcatttgtct acaaaaaaag atttgtatat gatacatgga gtaacaggtc
     4261 caaacgagta tgaaaacaat atcaataata attggtatac taattatata gctaaatggt
     4321 gtttagatta tgctgtagaa aatatcttac gattagagaa agagtgtaat gaatgtattg
     4381 acagaaataa tgtgactagg gaagaagttg agttttggaa aataatatca aataaaatgt
     4441 atctgctgta tgatgaggaa ctacaaataa taattcaaaa tgataatttt ttagataaag
     4501 aatttgtaga agtaaaggat ttaccaaaag aaaattttcc tcttaatcaa aattggtctt
     4561 gggataagat tcttaggtcg tgttttataa aacaagcaga tgtattacag ggaatttact
     4621 attttggaaa tagatttact aaagaagaaa aaaagagaaa ttttgacttc tatgaaaaat
     4681 atactgtaca tgaatcttct ttatcttcta gtatttattc tattattgcc tcagaaatag
     4741 ataacctaga aaaggcttat gaattatatt caagaacagc taggcttgat ttagataatt
     4801 acaataatga tacaaatgat ggactgcaca taacttcaat gtctggttca tggctttcca
     4861 ttgtacatgg atttgcaggc atgagaacat ggaatgaaac tctatctttt gaccctaagt
     4921 tgccaaaaga gtggaaaaat tattctttca atataaatta tagagataat attattaatg
     4981 tagatgtatg tagtgattta attaaaatag aaaatttaaa aggtaaacca ataaaaatta
     5041 ttgtatatgg gaaaaaatat atattagaaa aatgtataaa aattgataaa tagatttaat
     5101 aatattttta ttatataaat agttaaaata agctatctaa ttatttattt attttaacta
     5161 ttttgtgtga tagaaaacta tatattataa caatagtagt gcttttaatt aaatatattt
     5221 cataaatctt cataaaattt tcaaaatttc gtaaagaata tgtcacaaaa gtaaggtata
     5281 ttatattcag cagatagtat aagggagg
//
LOCUS       DAGSWI010000194         5302 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.198, whole genome shotgun sequence.
ACCESSION   DAGSWI010000194 DAGSWI010000000
VERSION     DAGSWI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.198"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            322..1848
                     /locus_tag="MJF80_003622"
     CDS             322..1848
                     /locus_tag="MJF80_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBZ0282231.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1876..2433
                     /locus_tag="MJF80_003623"
     CDS             1876..2433
                     /locus_tag="MJF80_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282232.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2955..3998
                     /locus_tag="MJF80_003624"
     CDS             2955..3998
                     /locus_tag="MJF80_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282233.1"
                     /translation="MLKKWFGIVKKRQKSESAKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VEGEKIDLNKFEGLDQNENYNLEKNVIEEKEVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            4109..5251
                     /locus_tag="MJF80_003625"
     CDS             4109..5251
                     /locus_tag="MJF80_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBZ0282234.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYIFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2247 a          434 c          942 g         1679 t
ORIGIN      
        1 tatattgtaa tattaaaaaa ataatagtaa actgattgca taaaaatgat ttttatataa
       61 aatttaagca gaagaataaa tttctaacga aattggatat ttcttctgta aatacatatt
      121 caaaaaaaga attataagag tttagtcagc agtcgtgtaa gtaataagga ctttataaat
      181 atatagaaat ataatgatat taatataacc aagcgatttt taatttagct ttttaataag
      241 cttatttttt atactattta taataaataa actccatgat gggaaaacta ttactattta
      301 atatgtaaag gagtatttta aatgaatgaa tcatattggt ttttaaactc aagtccaaaa
      361 gaatataaca aattaggtga aaatataaaa acagattgtc ttatagtagg tggtggaata
      421 acgggattga caactgcata tttattagca aaagaaggta aaaaagtagt gcttgttgag
      481 gctgataaaa taggatatgg aacttctgga aggaatactg gtaaggtaac ttgtcagcat
      541 gatatatttt actctaagat agagaaaaaa tatggattag ataaagcaaa atcatattat
      601 aatgcaaata atgaagcttt aaatttagtt gaacaaataa tagaagaaaa caatataaaa
      661 tgtgatttta aaagggaaac ttcttttgta tttacagaaa aagaggatac tataaagaat
      721 attaaagatg aatatcgtac atgtaaaaaa ataggaataa attgtgagta tcatgaaact
      781 atagaaaata ttccattaga tataaagggg gctattagtt ttactaatca aggtcaattt
      841 aacccaaaga agtatattga tggattggca aaagcagcag taaatttagg gctaaaaata
      901 tatgaaaata caccagtagt agaccttgaa aaaggaaaaa tatgtagggt taaaacaaga
      961 gaggataata taatagaagc tgagaatgtt ataatttcat ctcatagtcc atggtatgat
     1021 ggattgaatt tttattttgc aaaagaatat gcagagcgtg cttatttaat ggttgcagtt
     1081 ttggaaaata aattagcaga tggtatgttt ataagtatag atgacccatc aataactttt
     1141 agacaatata atgatggaag tgaaaattta ctgatttttg gaggaggaga ccataaagtt
     1201 ggtcagggag gaactgagaa agaaatattt gatgatttag agcattatgg taaagaagtc
     1261 tttaaggtaa aagattttaa ggggaaatgg tctgctcaag acaatatgag ttttgacaat
     1321 gttccttata ttggttatat caacaaaaga gaagacaata tttatgtagc tacagggttc
     1381 tctaaatggg gaattactaa tggaactgct gctggaataa taataaaaga tttgataata
     1441 aataataatt ctgattataa agatactttt aatccatcca gactaggtag ctacttttca
     1501 aaagatttta taaaggaaaa tgcaaacgta gcaataaatt atgtaagtgg aaaattaaaa
     1561 ataggtagtg gagatatgcc taaaaataat ggagaaggaa agattgttaa tattgatgga
     1621 aaaagatatg gtgtatataa ggatgataat ggagattttt atattgtaga cactacatgt
     1681 actcatttag gatgtgaact taattttaac agtgaagaaa aaacatggga ctgtccatgt
     1741 catggttcta gatttgacta taagggaaat atacttgaag gaccagctct taagccatta
     1801 aaactttatg gacatggtga taatgatgtt aatcctaagt tattataaaa ttatttttag
     1861 gaaggtaaag tgtatatgaa taaactttta aagccaataa ctatactatt gtatatagtt
     1921 tatattttat ctgccgtatt ttttgtagca aacaaagaat atggaagtct aggtctggta
     1981 gcaattagtt tagttggtac tttcattttg agttttataa ataagcaaac ggaaagatta
     2041 ttgagtaaag aattatatat aatgcttgct ttatttataa tgttttcatc attgttaggt
     2101 acttgtttta atttttatga tataaattat tatgatgatt ttttgcattt atggtcaggt
     2161 ataatatctt gtattgtggc tttttctata attaaatact tttatacaca aagagagata
     2221 agtaaaatga gtaaagtgtt tttagttata tttttattta tgtttagtat gggagtagcg
     2281 agtttatggg aaataggtga atttttaatg gatacttttg ttgggactac tacacaggcc
     2341 ggagggttag aagatactat gattgatatg gtagatgctt taataggcac aattataact
     2401 attcctttta tagtaaaaaa atctaatata tagttttaaa ttttttatat taattataat
     2461 ggatttaatt aatcatgttg agtttcaagg tctatatgat taattaaatc catttttgtt
     2521 atttataatt attattgaat atatttttac atataagata taaggtgata gatagaaatt
     2581 gttggattat gtaaataatt aatatataat accattatgg gcaataaata caaatcaaat
     2641 gataatatag tctatttatg taaatgttat attgtatact aagtataata gtaagttttt
     2701 tgatagtggt gtagaaagta aaaatctact agcctaaatt gatgtatgga ttagtttagg
     2761 aacagtaatt gcatatctta agtttgatgt catgttgggc tactagaaat aggtcatctt
     2821 tttcacttga atatcttttc tttaaacatg gagagtatca tatcaaaaac atatgaaaat
     2881 taagtgattt ataagaacag taatatttta tatgatagta tagaatagta ataaagtatt
     2941 aggaggaaca ttgagtgtta aaaaagtggt ttggtattgt gaaaaaaaga caaaaaagtg
     3001 agtcagcaaa agaagaaggt gaagtaatat taaaaaacga aaaaatatta tctgaagaaa
     3061 agttgataga tgaagaagga gttgtagtta atattgataa tgaaatatta actaaagtag
     3121 aagtagtaaa tgatgataat gaaatagaag aaaaaataat agaagaagat tggttaatta
     3181 gtgaaaatac cataaaatta gatgataaag aagcaattat taatgataaa aacatagaat
     3241 tatgtaaaaa agaagttcaa gttgaaggtg aaaaaataga tttaaataag tttgaaggac
     3301 ttgaccaaaa tgaaaattat aatctagaaa aaaatgttat tgaagaaaaa gaagtaagtg
     3361 aatgtttgac agaagaagat ttagagtaca taaaagaaat taaaataaaa agaggaaaaa
     3421 gtataaaagc tataaacttg tatactaaag aagagtgggt ttttgacact catatacagt
     3481 gtagtaaaaa actcaaagtt ccattagggt acataagaga aaatttaaaa tatggatata
     3541 tggattactt tggagatgca ataaattatt taagtgaagt attaaatata gatgaatact
     3601 gcaaaagtga atggagctat ctcgataata gcaaatctcc atctgagata tttaatattc
     3661 taaacaataa aatatttagt ataaggcttt ctaatgaaaa aagaaatgaa attttgacaa
     3721 atgataaaat tgaagcatta aaaatgaatt atagatttga atgtattgat gaagaatatg
     3781 atgaatattt taaaaaatat aagtctataa tcaaaagagg tggaaagaaa aaagttgaat
     3841 tagtaaataa aaaaggtgac attttagaaa tatttaagtc tttagaagaa tgtgccattt
     3901 atttgcaaaa ggaaaagaat gaagttatac aaatgttaaa atatggagat acaaaagtag
     3961 gaagaaactt tataaggtat agtttgagaa gtatttaatt tcattttaaa ttcataagag
     4021 tttcatttct gattcacaca tttaatatta ttttaatgta aaataataaa ggtgaagtat
     4081 tgtacaatta aaattaagga gggtattaat gtctgaagaa aaaaatccaa aagaaaatac
     4141 attagatgaa tctaaaaatt cagataatgt aaattctaaa aaagatgatg tgtcagatga
     4201 aaaaaatact gataacaaag ttaaaaaaag ggaaataaag ctaaacaaca aaacacttaa
     4261 agctattgta gcaattgttg cagtaggagc tatatgttta ggtggaggct atatttttgg
     4321 caaagaagct ggaagaaaat tgcctgctac ttcaaaacac tataatagca gtgaggtagt
     4381 agctactatt ggggataaaa aaataacagg tgaaatgtta agaaagagaa tggagccatt
     4441 attttattta aatgcaaaaa ctactatgac tgatgacgaa atacgttcat atgaatcaaa
     4501 tatgattgaa actatgatta catctgatat attatcctct gaagcagcaa aagagaaaat
     4561 aagtataact aatgaccaac tagatgctca gtataataat ttaatgagta gtatagaatc
     4621 agcaatggga atgactaaag aagagttttt aaaacagttt gatttgactg aaaaatatat
     4681 aaaagatgat atgaaaaaag agctagtagc atcaaaatat ttaaatgaaa aatcaaaagt
     4741 tactgataaa gaagcagaga actattacaa taaaaacaag agcaattatt tacaagttag
     4801 agcttcacat attttaataa aaactgttga tgataaaggt aagcaagtta gtagtagcaa
     4861 aaaagctgaa cttaagaaag aagctgaaga aatacttaaa aaagcccaag ctggggaaga
     4921 ttttgctact ctagctaaaa aatactctga agatagtagt gcagaatcag gtggagattt
     4981 aggtttcttt ggtaaaggtc agatggtgga atcttttgaa aaagcagcct ttgcattgaa
     5041 gaagggagag gtatctaaca aacttgtaga aagtgattat ggatatcata taattaagaa
     5101 aactgatgaa aaatatcagc catttgaaga ggtaaaatca gatttagtaa gtagtttaac
     5161 atcagaaaaa caaaacctac ttatacaaaa tctaaaagaa aaatataatg ttaaaattac
     5221 agataaatat caaatgaaag ctaaagaata gataaagtca atttattaaa aaaaagaaaa
     5281 attaactgta tggataaaag ct
//
LOCUS       DAGSWI010000195         5255 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.199, whole genome shotgun sequence.
ACCESSION   DAGSWI010000195 DAGSWI010000000
VERSION     DAGSWI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.199"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(196..2475)
                     /locus_tag="MJF80_003626"
     CDS             complement(196..2475)
                     /locus_tag="MJF80_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBZ0282235.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2746..4200)
                     /locus_tag="MJF80_003627"
     CDS             complement(2746..4200)
                     /locus_tag="MJF80_003627"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBZ0282236.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4364..5233
                     /locus_tag="MJF80_003628"
     CDS             4364..5233
                     /locus_tag="MJF80_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0282237.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
BASE COUNT         1790 a          824 c          561 g         2080 t
ORIGIN      
        1 ccatatactc ttacctcaaa aacattcaaa tgtaaaaaat actacatatt aataaacttt
       61 gcccaataat tcctaaaata atatcctaac aatgatttat gtttttataa tagtatattt
      121 ttacataaaa aagtgaacat tgattaacaa agttcacttt tttataaatt tttatgttat
      181 aattcacaat tttatttaat ttgtttcttt tctatttaaa ctatatctat ctaatattaa
      241 tttttcatat tcttctattg gcattggtct agcaaataaa aatccttggg ccatatcaca
      301 acctataaac cttaaaaact ctgcttgctc tacagtttca acaccttctg aaacaacttt
      361 tatatcaagt tgctttgcca tttgaacaat acttgaaact acaatttgac tcttttttgc
      421 atttcccact gttataaaga attgcctatc taatttcaat acatcaattg gcatatcttt
      481 aagcatattt aaagaagaat aaccagaacc aaaatcatcc atagaaattt taaatccaac
      541 agatttcaaa gtttgcatta ttttaaataa tatttttgta ttgtcaaata ttgcactttc
      601 tgtcaattca atctcaatta aatttggttc aatttcatat tcttttgtta ttctttcaag
      661 ctcagaaata aagcctggat tctcaagatg tactcttgat aagtttattg atataggaaa
      721 tgtggggaaa ttttcatttt cccaacgttt aaaattttta caaacttggg taaatacaaa
      781 catatccaat tctgttataa aaccattttt ctcaaaaata ggaataaata cacctggact
      841 tattagacct tttttgggat gttgccaacg tacaagtgct tcagcaccta caattttagt
      901 agttgataaa tcaaccttag gctgtaaata aactttaaat tgttcatttt ttaaagcatc
      961 atgcatttct tgttcaatat cacgttcttc tatgagagtt tcttttaaat tttcagcata
     1021 aatctcataa acttttaaat attttctttt tactgtagtt tttgcaatca tagctttgtc
     1081 aagacaggta gaaatatttg tttctccctc ttcaacaaaa tatacaccta tggatggaat
     1141 taaattgagt tctactccca aacttgtttt tatcattgac aattttttac gtataagttc
     1201 acatatttct ataattgact ctctattatt tttcttttca aatattatag caaaattgtc
     1261 atttgaaatt cttgaaaata cagattgctc tttaaatata ttcttaatta tctctgctat
     1321 ttcctttaat actctatctc cttcttcata cccaaaccta tcatttacta gcttaaactt
     1381 atctatatca aaataaatta aaactagatt ctttttctca tgagaggata aaaattttcc
     1441 cccttcaaga ttaaatttat ttgaattgcc aatacctgta acgttatctt taaaagcaaa
     1501 ttcttccaat ttttccttac tctttttatg tataaatagt atataaattg ttaatgccgt
     1561 aaagattata atcaagacta cacatgctga taatgtaagt tttataatag catatgattt
     1621 ctcagatata gcagttttag gaacttcaga aaaaagatac caatcattga ttcctattgg
     1681 ggcataactt aaataatatc cttttccaaa catactataa cttaatgaac ctgatttaaa
     1741 gttttttata tttgacttca tattgttgat actagatttt gatattgaat cacttctgcc
     1801 aatgtaagaa aataaatttt ctttagtaga gttggcgttt ttactgcttg gatttaaaac
     1861 aatatcacca ttacttctag ttatataaga atatcctttt ccattaaatg taggtacaga
     1921 aagtattttt tcatacaact tcgtactatg tgtggcaaat ataactcctt taacctcatt
     1981 atctttataa attggtacag aatatacatt aatattacta ttggtatttt tatcatcttc
     2041 tattacatct tttaattttc ctgttataac cacttctcct tgcattgatt ttaagaaata
     2101 atctctttta gagaagtctt ctatataacc atctgtagtg tatgctgtac catttggtaa
     2161 aataactccc attcttttaa atgaactatt aatagcttgt ctttttagta tagaaaggat
     2221 attatctact tcaaaagact cttctccttc aataaatatt gaaattgact ctagtaactt
     2281 tatatctcca ttaatttgtt tttttaacac attgacgctt tgatttgcta tttctgaaag
     2341 atattgattc gtttcttcac ctaatgtctt attcaacctt gaaccataaa tcaaacagga
     2401 aattgtaatt actaagatac ttatgacagc aattatggac aaaccatacc tattattttt
     2461 tgcatcactt aacattatgc ttcctgcctt tctcgaatct atgtatgtat aatcattatc
     2521 ttatgacaat tattgtaaac attttataat aatacttatt tttccatatt tactgtaatt
     2581 atacaaaatt ataaacttta ttacaacaat aaattaatta atatagatat tagtgtttac
     2641 attattgcat tatctatttt acaattattt acatataaaa aaatgtaata tcaaagtgaa
     2701 gcttgatatt acatttttgt tcttgtattt tgtttaattt ttatgctaca tcttacttaa
     2761 acactcattt gcaacttctt tatcatcaaa atgatgctta actttaccta taatttgata
     2821 agtttcatgt cccttacctg ttataattac aacatcatcc ttttttgcca tacttatagc
     2881 ttctgctata gcttgttctc tatcaacaac aacatggtaa ttttcttttt tcttgtcaat
     2941 tccttcaagt atatctttta ttattgcctc tggttcttca gttcttggat tatcagaagt
     3001 tataatacat aaatcagaat atttttgtcc aattgctccc atcaaaggtc tcttttcagt
     3061 atctctatct ccaccacacc caaacacaga aattattttt ccttctgcaa attgttgagt
     3121 tgtctttagt acattttcaa gtgcgtcagg agtatgagca tagtctacaa ttacgcttat
     3181 acctttatca tttggaactg tttcaaacct tcctgatact ccattagaaa gtctaaggcc
     3241 ttctttatat attggtttag gtatgccaag tacatagcaa gcagctataa ctgctaaagt
     3301 attatatact gtaaacatcc ctggaactgg tataaatatt acttcttcat atgaaggtgt
     3361 tattaatcta taagatactc catccgaatc tgattttata tctctagcca taaaatcagc
     3421 cttagtatca acaccataag tgtaacaagg tacatttata ccttttatat tttcatatat
     3481 cttttttcca ccttcatcat ctatgtttat tatatttgcc atagtagtct taaaaaataa
     3541 tttttctttt gcttttctat aatcctctaa atctttatga aaatctaaat ggtctggtgt
     3601 taaatttgta aatatgccta gtttaaagtc tgtttcatca actctattta atgccaatga
     3661 atgtgatgaa acttccattg cacagtagtc acatccatta tccaacatat tattaaaatg
     3721 atattgtaaa tctatacttt ctggagttgt tgaattggaa acaatgtctt tttctccatc
     3781 aaaaattttt atagttccta taagtccaac tttattctta tttaaagtta atatctcatt
     3841 tagtatagta gttatactag tttttccatt agtacctgta actcctataa cgttaaattt
     3901 ttgagatgga tgattgtaaa aattatcagc tacttttgcc atatcttttc ttgtatcttt
     3961 tacctttata aatgtacatc cttttattgc tacatcttct tcaacaagaa atgcacttgc
     4021 tcccttttct atggcatctt ttatatactt atgaccatct gacacaaatc cttttataca
     4081 tataaataat gtaccttttg taacctttct tgaatcatat tgtacattat taatatctat
     4141 atttaattca cccttgacat tgattatatc caacccctgt ataatatcat ttaatttcat
     4201 aaatgctttc ctccctttaa actctttctg ctaaaattac actacatata atattatatt
     4261 aagaattttt tactatgtga atggttaaaa atgcttgaaa cattgcaaaa aatgctctat
     4321 aattttctat aatataactg aggaaaggtc ttctataaaa actatggata ataaaatcgg
     4381 gtacttaaac gataacttta aaatatttaa tattagagat aaaaaagata tacaatttga
     4441 ataccataat catgatttta ataagataat tatatttata aatggaaatg taacttattt
     4501 tgttgatggt attccctata aactaaagcc ttgggatata ctctttatca gcaataatga
     4561 aatccataaa ccagttatag attcaaatgt cttttatgag agaattgcaa tatggattag
     4621 cccagaattt atgaaaaaaa atagtgatgc tagtagtgac ttagaaaaat gttttaaaat
     4681 agctacagag aataaatgca atagattgcg tttaaatatg catgatattg aatctattaa
     4741 gagttttatt gagcaaataa atatttccga atccaatact gaatttggaa atgaaatatt
     4801 gagaaataca ttatttatac aacttatgat attcttaaac agaattttcc taaaaaatga
     4861 aaatatggaa tcaacagaag atgtttttta tgatgaaact attaagaaag tattaaacta
     4921 tataaattca aatatagatg ataagctttc aatagatagc atatcgtctg aattttttat
     4981 aagtaagtat tatcttatga gaaaatttaa atctcatact ggaacgtcta tacacaatta
     5041 tatagttcaa aaaagattaa ttttggcaaa gtcactaata accaatggtc accttatgag
     5101 tgaagtttgt aataaatgtg gattcaacga ttattctagt tttgtaaggg catttaaaaa
     5161 aaactacggt gtatctccta aaaattatat aaacaataaa aatcctttag atattactct
     5221 tttagatgaa tagataattt atgtaaattt tattt
//
LOCUS       DAGSWI010000196         5246 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.200, whole genome shotgun sequence.
ACCESSION   DAGSWI010000196 DAGSWI010000000
VERSION     DAGSWI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.200"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            20..823
                     /locus_tag="MJF80_003629"
     CDS             20..823
                     /locus_tag="MJF80_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282238.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            835..1965
                     /locus_tag="MJF80_003630"
     CDS             835..1965
                     /locus_tag="MJF80_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0282239.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISRSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            1969..3378
                     /locus_tag="MJF80_003631"
     CDS             1969..3378
                     /locus_tag="MJF80_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282240.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3589..4098
                     /locus_tag="MJF80_003632"
     CDS             3589..4098
                     /locus_tag="MJF80_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBZ0282241.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2185 a          438 c          880 g         1743 t
ORIGIN      
        1 atagaaagaa aggcacatta tggaaaaatt gagagttatt gaaatgataa aaaataatcc
       61 caatattata gctacaatag ataatccaac tgatgaaatg aaattgttag ctattaaaga
      121 aaatgggctt gtactagaat atataaataa tccaaccaga gagatgcaag aactagcaat
      181 agataataat attagagcaa ttaaatttat agataatcca acagaagata tgatgataaa
      241 agcagtaaat gagggatgga gtatactgga ttatatcaaa aacccaactg ataaagttat
      301 tgaaatggct ataaatcaag caggatgggc aattaaatat gctaaaaatc caagtgaaga
      361 attacaactg ttagcagtaa gaaaaaatta tgattcgata aagtttataa aacaacctta
      421 tgaaagtgta caagaggagg cagtgagaat aagttatgat gccttgagat atatagattc
      481 acctagttat aatgcagaac ttatagctat aaaaaataat gaagcagcta ttagctttat
      541 aactgattta gataaaaata aaatgttgga atttttaaaa gtgaatattt tagtgattaa
      601 gtacattctg aaaaaagtat ctaaagatga attagagagt gtattaaaag aagtattatc
      661 caaagaagat gttgaggaaa agtatattag agattttttg aattgtagtg ttatagatag
      721 aaatagtgaa agtataccaa tagataaaat tatgtttatt tataaatatg gaagtaagac
      781 agcaaaaaaa atagctgtag atgagaaatt aaagattatg taggagagag aaaaatgaat
      841 tttatagaca cattaaaaag cgttgactta atattatcca ctggagaagt acctttaata
      901 gtaggtgaaa gtggaatagg aaaaacagct ttagcaaata agcttgccaa agaaaatgat
      961 tggagtttaa ttgttattga tggaaactta cttaaagagg gtgaaatagg tggtcttcca
     1021 actatagaat cttatgtagg agttaattct aatggatata agactgaaaa gaaaacaaca
     1081 gtgtatgctg ttcataacaa attaagagaa attgatgaag agatatctag aagtaagaca
     1141 gttcttttat ttatagatga gataaatcgt tgtgagcata cagtacagca agaacttatg
     1201 aatttaattt taaatagaga aattaatgga tataaactgc atgatgatgt aaagatttta
     1261 gcagcaatga atccatcaag taaatatggt tcagattttg attatcaagt ggtagatatg
     1321 gatgcagccc aagaaaatag gtttgtttgg ttaaatatgg agagtgacca tacacaatgg
     1381 ataaaatggg caatagatga gggtattgaa agaaaagtta tcgagtttat ttccactttt
     1441 ccagaatatt tgcataaaat aaatgaagat gatgtaagag ctactcctag aagttatgag
     1501 agagtatcta aaatttataa agtttataaa gaaaaaaata attctattcc tagagcagta
     1561 tttttaaatg ttgtaaaggg taatgtaggt aaagttattg cagaagaatt tataagtttt
     1621 attgaatcaa actctgaacc attaatatcc tatgaagatg tttttttagg agaatctatt
     1681 gatgagtcca ttgtagaaag agtaaaaaat gagagtcata caagacttta tctatcagct
     1741 atgaacattc taaaagactt agaattaaat attaagaatg ataaatatga atcaaaccac
     1801 tatattaata gatttattga atttttaaaa atgtatcctg tagatttaat gataggtatt
     1861 atgaaggata ttagaaacag ttatattgaa gtatataaaa aagctataga aaatgaagag
     1921 tttgtaaaat catattttga atcttatagt ttaataaggg gataatgtat ggaaaattat
     1981 tttgataaac aagcaaaaga gctttataat aaagcaagtg aaattataaa tactcattct
     2041 atgctaaagg caaatcgtgc taatgagaaa tttgatatag atataccaca agattttaag
     2101 tatgagtttt ttagtcttgt agataaagtg aatttaagtc ttatggaaga agaagataat
     2161 ttttatggat attttttgtt tcagatgtca agagaaataa gatttgatat aagtagtcct
     2221 acaggtgtga attttaaggg agctaaatat attatatatt ttaatccaat aatcttttta
     2281 acgcttgaca taaagcaaat ggagactaca attaaacatg aaattcatca tatattatct
     2341 atgcatttga tgagagcaaa ggagttaaaa ggaaagtata gtacattagc aattaatatg
     2401 gcaatggata tagtagtaaa taaatttcta aacaatttac caccatatgc aactacctta
     2461 gagtgggtaa atttaaagta ttccttaaaa cttgaaccat atgaaccttt tgaatattat
     2521 gtagaaaaga ttcagactga attagatttg atggaagtgg atgaagatgg tgaggaagat
     2581 gatagtgata aatatggaga tatagaaatt gattatagcc ccgaaagaac tcatgatata
     2641 tgggatgaat ccaatgagat agatgaaaat acccttagag aatttactga aaaatttatt
     2701 aacagttctc aaaaaggtaa agttcctgct tatttagaaa gtatgatatc atcacttaaa
     2761 aatagtaagg gtgaattgcc atggaattta tatcttaata gaataatggg aacagttgaa
     2821 aataataaaa agaagactat aacaagaaga aatagaagac aaccaaatcg tttagatttg
     2881 aggggagaac ttagaagtca taaggcagaa atagcagttg cacttgatgt aagtggaagt
     2941 attagtgatg aagagtttaa acaagctatt aaagaagttc ttagtatagt gaaaaactat
     3001 aatcatgaaa ttacaattat agaatgtgat aatgaaatta gacgtgtata taaagtgaga
     3061 actgaaaaag atataaaaga tagaattagg ataggtggag gtactagatt ttctccagtc
     3121 tttgagtatg ctaataataa aaagattaat ttgttagtct actttactga tggtaaaggt
     3181 gaaaataaac tacaagtaat acctagaggg tataaaattt tatgggttat ttctggaaga
     3241 ggagataaaa tttcattaaa agaaccttat ggagcagtta aaaaacttac taaggttaaa
     3301 acaaatgatg aaatattaga aataaatgat gtaagaaatg atgggtactc aatgaatagc
     3361 caagcaccaa ttttataggt attcacagga gataagcttt attaatttat tttgatatgc
     3421 ttgttataaa aatctaaaaa atacattaat caatggttaa tgtatttttt aattttaata
     3481 atataagttt tacttacatt taagtcttaa aatgtgttaa aatataatat atagcagata
     3541 gttttggcaa ggtggattta gttgtcaaaa ctattttttt tattaaggat ggtgatgttt
     3601 ttgtttaaga ttgatgatta tataatgtat ggtatgacag gagtctgcaa agtcatggat
     3661 ataacaaatg aacgatttac aaatggaata aaaaaagagt attatgtatt gagtcctatt
     3721 tattctaata atacagtaat aaaaattcct gtagataatg aaaaagtacc aatgagaaag
     3781 ttgctttcta aaattaatgt gctttcatta ataaatgata ttcctaatat ggatacatca
     3841 tggatagata atgaaaaatt aagaagtgag caatttaaaa aaatactcag aggtggtaaa
     3901 tgtgaagagt taataaaatt agtaagaagt atagataata acagagagta tgtaaaatct
     3961 attggtaaaa aaacacatca agcagatgat aatataatga aagaagctga aaggttatta
     4021 aatgaagaat ttgcaactat tttagatatt tctcctaatg aagttagttc atatatttca
     4081 agtcatatac ctcaataaag tgttttagta tcctttattt aatctatttt aagtattatt
     4141 tatgtatgct ggttttggta aaataaaata tataattaaa ttatataaaa aataataatg
     4201 attgtattga agaattacag aatagtgcgg agagcccact cctttaggtg tgagatggat
     4261 attacttaaa gatttaaatt gttattgtag atgtttttgt actttttaat ataaagcaag
     4321 attaataagt gtattgtaag ctttaagtga aattgatact ataatgagtt acttagattt
     4381 taaaatatat aatattataa aaaatccttt taggttttga atgaataaga tggatactat
     4441 gttgaataga atccatctta tttaagtttt atttatgttg tttatttgta agcattattt
     4501 ttgtacatga aaacacagtg taaaaatatg cttagaatta tgaagtttta ttaaaaacat
     4561 attcgacaaa atgagactaa aaatattctt aaacttataa ctaagataaa tattgtatat
     4621 ttatagtgta gcctgaaaaa ttgtggatgt acgaaattta tttagtattt tattgaaaca
     4681 aagtagaaga agtactatag tttatttttg aatagattgt tattttttag tgaaaaagtt
     4741 attaggttaa tacataaata taatagttta taaatagttt aagcatgtta aaaaagtagt
     4801 tattagagat atatagttac attgcttata agatacttaa aatgatagca ttcacttata
     4861 tttagaaaat taagataaaa atcattttta tatagtaaag catacctaaa tttagaaaat
     4921 aattgaattt ataaaattaa ttaaatacat aaaaaagtat tcaatcaact taacaaattt
     4981 ttttaaattt aatttaaatt ttttattcta ttaattatta atattatttt caaatcaatg
     5041 cagttataag aagtgtataa tttgattatt atatactgca tacaaaaata tatttgagca
     5101 aataagtaaa gtaataaaaa aatcaagatt tcatatctaa aaacaattct gaacattaat
     5161 ttaatttata ttttcatagg aaatatttta tataatatac ttgataaata taatttgaat
     5221 ttttctaatt atttactgtc gattaa
//
LOCUS       DAGSWI010000197         5236 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.201, whole genome shotgun sequence.
ACCESSION   DAGSWI010000197 DAGSWI010000000
VERSION     DAGSWI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.201"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            99..1562
                     /locus_tag="MJF80_003633"
     CDS             99..1562
                     /locus_tag="MJF80_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBZ0282242.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHEGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1618..2754
                     /locus_tag="MJF80_003634"
     CDS             1618..2754
                     /locus_tag="MJF80_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBZ0282243.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            2990..4106
                     /locus_tag="MJF80_003635"
                     /pseudo
     CDS             2990..4106
                     /locus_tag="MJF80_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            4258..5127
                     /locus_tag="MJF80_003636"
                     /pseudo
     CDS             4258..5127
                     /locus_tag="MJF80_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT         2018 a          540 c          984 g         1694 t
ORIGIN      
        1 gattttttag atatattaat ataaagatta tataaaatat tgaaatatag ctgtttaaat
       61 tattgtctat aaatataaaa tgaatggtgg tgagttaagt gaccgattta gttaacacca
      121 ttaatggaat tgtttggtca cccgtattag ttgcaatgtg tttgtgtata ggattatatt
      181 tttcattgaa attaaaattt tttcaaataa gagatgtaaa agaaatgttt actctcttgc
      241 ttgaaggaga tagttctcat gttggtatat catcatttca aggttttgct acatctatgg
      301 ctggaagaat agggactgga aatatagctg gtgtagctgt agcaatagca atgggaggtc
      361 caggagcatt agtatggatg tgtataatgg ctacattagg ttcagcaact gcatttgtag
      421 aatctacttt agctcaaata tataaagatg agcatgaagg gcagtataga ggaggacctc
      481 catattattt tgaaaaaggt cttgggtgga aaaagtgttc agttttattt tcaatagttg
      541 caataataag ttatacattt tttatgcctg gtacacaatc aaacacttta tatctagcaa
      601 tgaatcaagc atttggattt tcaaaaatga caatagcagt agcatcttct attgcattag
      661 cgataataat ttttggagga attaaaagga ttggtttatt tgctgaaaaa gtagtaccta
      721 taatgggtgg agcatacctt attatgacat tagtaataat tataacaaat attagtttag
      781 ttccagaggc tattgtaaca atgtttaaat ctgcatttgg atttggttca gtttttggag
      841 gaatgctagg ttcagcaata tcttggggag taaaaagagg tatatactca aatgaagcag
      901 gagaaggttc tgggacttat ggtgcggcag cagcagaagt aagtcatcca gccaaacaag
      961 gattagttca agcattttct gtttatgtag acacattatt aatatgtatg gctacaggta
     1021 ttatgattgt aataacagga atgtataatg ttacaggtgt ttgcacagca ctaccaggtg
     1081 tagaaccggg accacaatat gtacagagtg caataaatac attacatcca caattaggtg
     1141 atatttttat agcaatagtt ttattattat ttacatttac aactttatta gccttctcat
     1201 atatgatgga gactaatgta tcttacttag taactagaat taaagcagaa aaacatgaaa
     1261 agtacataat taatgtctgt agatttatgc ttatagcatg tgcagcaatg tgttgtttta
     1321 tagaaccact tacaagctgg gcattaggtg atatatgtat aggtgtatta acatatataa
     1381 atttaattgc catctttaca ctaaaaaaac cagctataaa ggcatattat gattatataa
     1441 gacagaagaa gatgggaata aaaaggagtg atagaacttt taatcctata gagttagata
     1501 taagaaatgc tgagttttgg gaaaatagat acttaaaaga ggataagcat gagttaaatt
     1561 aaagttataa taaaatataa aaattactaa ataaatttag tgggaggtaa gattggtatg
     1621 aaagaaagaa taatacaagt ggctgattca aagaaagaaa aaattctagg gctatgtcaa
     1681 tctttatatg atgaaccaga aattgctttg caagaatata aatctgctaa aaaaatatca
     1741 gaatttctaa gagaagaggg ttttgatgtt gaggaaaact tggcaggtat ggctacagct
     1801 tttaaagcaa ccaagaaaaa tggagatggt cctaaaatag catttatagc agaatatgat
     1861 gctcttccag gcaatggaca tgcatgtgga catcatttaa ttgcatctat gggagttgga
     1921 gctggaatag ctctttcaag tatactggat acttacaagg gagaagtaag tataattgga
     1981 acacctgctg aagaaactgg agatggaaag ccttacttga tagaacatgg tgtatttgat
     2041 ggctatgatg cagcaatgat gatacatcca aattcaaaaa cttgtgtaac acctgaaatt
     2101 atagcaatag gaggtctaga tttcatattt actggaaaag catctcatgc aggagctaag
     2161 ccatataacg gaataaatgc tttagatgca gtagtactac tatataataa tataaatgca
     2221 ctaagacaac aattagtaga tggaacgaga atacatggta ttatactaga agcaggaact
     2281 gctgcaaatg taatacctga tatgggaaaa gtaagattag agataagggc aaaagaacaa
     2341 aattactttg atgaagtagt agaaaaagtg aaaaattgtg ctagaggtgc tgcaatagcc
     2401 actggatgtg aacttgaatt ttatcacttt gaaccaactt gccaaggatt aaatgaaaat
     2461 aaagttttgg tagacatatt tacaaagatt atggaggaat ttggaatata tgaagatgaa
     2521 caagtaatgt taggttcaac agatatggga aatttaagtc aaataatgcc atgtatacat
     2581 cctttgatga agttttctga aaatggagaa gaactacata caaaagaatt tttggaggct
     2641 tctataaatt catatgcaaa agatagagta attgatggta taaaaatatt agctttaaca
     2701 ggatttaatt tatttgaaaa tccagagtta ttgaaaaaaa tgaaggaaga gtagaactta
     2761 gactattttt aattatactc tttaaaataa gaagagtgtc tcaaaatgaa ctttttagtt
     2821 tatgagacac tcttttttgt atgaaattaa atatattttt attattttaa caatgaaaaa
     2881 gaggcttatc tgtttttata taaccttttt taacttgtag cacaaaatat catgaatgat
     2941 tacaattcga gtgataaaat cgattataag atatatagga ggggtacata tggaaacttt
     3001 agaagtaaga aatattgaat tgagtggtac aaattatgag ataggataca gactaggaga
     3061 attggttgct aatatgccag agatactaga aggtcagatt aataaatcta atgtggtaag
     3121 caaaaaaaga agaaaaagag atgatagagt tgtttgataa atattgtcca ggtcttaatg
     3181 aagaattgca aggttttgct gatgcaattc aagtgaattg taatcaaatt ttatattata
     3241 ctatgactta tcttaaacca ggttgtagtc aagttgcatt agcaccagaa ttaactgaaa
     3301 atgggcatgt actatttgca cgtaatttcg atttctcaca caatatggag gattttgttt
     3361 tatgtaaaac taaggtaaat ggaaaatatg cacatatagg tactaccgtt atgcaatttg
     3421 gaagaggtga aggtatgaat gaatgtggct taggtgtaag tcagtcatca tgtggtattc
     3481 ctgtaggcaa ttcagatggt ttaagaaaac cagcaattgt aggtttacag ttttgggcta
     3541 ttattagata tttgttagaa aattgcaaag atgtagatga agcattagaa tatttaaagg
     3601 atatgccaat tgcatacaat attaatttat tgcttgcaga taagtctgga aacattgcac
     3661 ttgtggagac tttagatggg aaaaaagaag taaatataat caatagttta gaaagtaaaa
     3721 gagaatattt tttacactct actaatcata cacatattga taaacttcat aaattagagc
     3781 cacaatcaat gaaaaattct attcatagat ataaattaat taaagaatat ataaataagt
     3841 ctaaaaaaat tggagagaaa gagttaatga atttattgtc ttcaaaatat ccaaatggct
     3901 tatcttgtaa ttactataat gatttttttg gaactttaaa aagtatagtt atggatttaa
     3961 atataggaaa gtttaatatt ttatggggtg gactagagaa taaatgggag agctactatt
     4021 taaaaaatga cattaaatct ataacacaga ggataaacat aaatattgaa aaagcaccat
     4081 ctgacttttt tgatttcatt gaataaaaaa tgtattcaat tattctaaat aattaggaca
     4141 tataaatcaa ctaaaagttt ttatttatcg tattgaatta gttataatat tttagtatag
     4201 taaaaaatag tgctatttta catatttaaa gagtttaagt agaagagagg tgccatattg
     4261 gattacttaa agtattttga aaaagctgta atgtatattg agaataattt acatgaaaat
     4321 ataactgtaa atgatgtggc aaaagaaact ggatattctt attatcattt aacacgctta
     4381 tttaagtcta tgtttggtga aagtgtagga agttatataa aaaaaagacg attagtaagt
     4441 agtacaaaag aacttttata tagtgataaa aaagtgatag atatagctat tggtagtgga
     4501 tttgaatctt cagaagcatt tagcagagca tttaaatcta tatacaaggt tagccctata
     4561 gaatatagaa aaaatagaat tgatgtattt gttggtaaaa agaaaaaatt agagttagat
     4621 tttatgagac atcttgttgg aaatataacg attaaaccag ttataaaaga aatagaaaaa
     4681 ataaaggtaa taggtataaa ggataaagta attttagaaa ataattcatt acctgacttg
     4741 tgggagaaat tcagaaaagt acatcatatt gtcccaaata ctttgccatc aaagagagtt
     4801 tttgaaatct gttaagcaat ctctgaaata cacctagtta gtgaaagtat ggaatttaat
     4861 gagataatag gacttgaagt taatagttat gatgtgattc caaattcatt tgtttcaaaa
     4921 actattaagg aaggaaagta tgctatattt actcatactg gtagcttaga taatttagac
     4981 aaaacttatg aatatatatg gggtacttgg tttttaagtt caaaagaaga acttgatata
     5041 agagatgatt ttgaagtata tgatgaacga tttttaggtc ctagtaatgt gaactcacaa
     5101 attgatattt atatacctat aaaataatct ataagaaaac taaaaaataa tttgattaag
     5161 aaatagatgt tcgtaaaaga gcatctattt tttattttta tcagatttag ataattttaa
     5221 taataaaaga aataaa
//
LOCUS       DAGSWI010000198         5213 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.202, whole genome shotgun sequence.
ACCESSION   DAGSWI010000198 DAGSWI010000000
VERSION     DAGSWI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.202"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            12..431
                     /locus_tag="MJF80_003637"
     CDS             12..431
                     /locus_tag="MJF80_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBZ0282244.1"
                     /translation="MNKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFLEMIDNEN
                     SYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPNITKYKEN
                     LINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
     gene            1059..2366
                     /locus_tag="MJF80_003638"
     CDS             1059..2366
                     /locus_tag="MJF80_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282245.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRRTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    2452..2714
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2840..3643
                     /locus_tag="MJF80_003639"
     CDS             2840..3643
                     /locus_tag="MJF80_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBZ0282246.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGETVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3681..4292
                     /locus_tag="MJF80_003640"
     CDS             3681..4292
                     /locus_tag="MJF80_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBZ0282247.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            4324..4995
                     /locus_tag="MJF80_003641"
     CDS             4324..4995
                     /locus_tag="MJF80_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282248.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         2118 a          472 c          895 g         1728 t
ORIGIN      
        1 tcaatttact tattaataaa ttatattcaa aaaatcataa tgaagcatat aaaacttttt
       61 tattcttaga aaatgaaagt ttaaaatcaa atattacata ttgttttttt gactcattcc
      121 tagaaatgat tgataacgaa aactcatata ttcgaacaag gggattgtta ttaatctctg
      181 caaatgctca gtgggatatt gataataaaa ttgaaataaa tatagatagt atactaagtc
      241 atatcgtaga taaaaaacct tctgtatcaa gaatgtttat taaatccata cctaatatta
      301 caaagtataa agaaaatctt attaatcgca taaaaatgga attaagtaat gcagatataa
      361 gtatttataa taataatatg aaaccattag tagaaaagga tataaacgat acattatcta
      421 atattagtta aatattaata tagaaattga atattatcta gttacaatta tttataacta
      481 agataataaa acaacaatgg tgctaattta tatgtataaa ttaattatga aaaatttata
      541 taagtaaaat atattaacat aaaggtaaaa ttttgtatac taaaagggaa tatttacagt
      601 ttaatggcag ttttaaaata ataaattaaa gctgtcattt ttatttaaaa atttttagaa
      661 aaagtacaat agtgttaatg tgtgaattag ataaggtgta tataatagtt tagatgatat
      721 gatataaaat atgttgtaaa ttaaatgttt tctttgacaa tatatacaca aggatgataa
      781 tatctatatg aataaaagat agtgtgtaat ttgataatat gtaattataa cattatgata
      841 taattacaat aaagtatgta ataatctgta gaaatttcaa gctagttatc taagtcggat
      901 aactaaatct aaacggaata cataatgtat taaataaatg atgatactaa aatgatacta
      961 tttttataga aatttattga ggtatataat taatgaaaat aatatattaa attatataat
     1021 atattgagtt ttacataaaa gcaaaggtgg gaataaaaat gaaagaaatt gctgatgcta
     1081 taacatcaga gtatttaagc tatggtatca atgaagatat tagtatctat gaaggtagat
     1141 tttgcatata tttagataaa aaatatagat gtaatgggaa aatttattat aaaatgacac
     1201 ctccaatatc tataagtttt aaagcagaca taggttgtgt tgaagagata gacaatgaag
     1261 atgacaattt agctttagat tatgataatg ctattcttga agttcatgga tataaaatta
     1321 tttctataac aataaatact ttaagtgaat ttagtgtaga aggttatatc aatgatgact
     1381 gcataaaatc taagaactcc tatgttgaat atgtagattt taatataata aacttagata
     1441 aaatacctgg aaaactcatt aaatacaatg ataaggtata cgcaggtaga attgaatttg
     1501 atataaatga ttatgttgta actatagaca agcgatatga ttatagaaaa gaattaaaat
     1561 cagaattaaa gtctaaatct ggagcaataa taactcatat aggtaggatt agaagaaaag
     1621 atggtagaat ttttagaaca aataatacaa taaatctttt agataggata tcaacagctt
     1681 tgagttttat gtgtggaaga tatgtagggt tttgtttggc caaagggtat agaagtggta
     1741 atgaagttta tagaatatgg aatgaaaatc aaatatctcc ttttagatat gtaccaacat
     1801 ggtcagatac actttcaaat tatcataata tggaaaaata tataagcctt atgtgtaaaa
     1861 agctagaaga tttttattat ggttctgcta taaagagtgt tgttgattgg tacatagaat
     1921 ctcttggaag tgctaccatg gaaaacaata taatatctgt acaaattgca cttgaaactt
     1981 tgtcatatgt aattctagtg gaacaaaata agatacttac agatgaagca tttgactgta
     2041 atttggcatc taaaaatata agactcttac ttgatacatg taagatacca tatggtaaac
     2101 atgaattaaa tatatttgat aatataataa aaaataaatt tgatgatgga gtggatttag
     2161 ttatttattt aagaaatagt atagttcatc caagtagaag gactcataga gctgtgttag
     2221 aggttgaaga tatttggaat ataatttcaa taggaacgag atatattgaa cttgttttac
     2281 tctttatttt aggttataga ggagaatatt caaacagatt agtagaaaga tgttatggtg
     2341 aagtggaagt tgtaccttgg aactagattt ttatatttat tttaaaatat atattgacat
     2401 atattgcctt atgctataaa atcaatatca taataataat tcagaataac attaaatgct
     2461 aagaaaagaa atagtaaaag tagttaatct taaacagaga gtttccgatt ggtgagagga
     2521 gcaatagatt ttactttgaa cacatctttg agcaggactc tgaaagagta gaaatacttt
     2581 attaggagat ttccggaatc cttgcacccg ttatagtgct agagtataat aattttattg
     2641 tacttaaaga ggttagtgtc gtgaggcatt aatgaacaga ggtggaacca cgagagttaa
     2701 ctcccgtcct ctagcagaag ctagaggcgg gagtttttta atataatttt ttaagcgcgc
     2761 agagaattat attgcaatag atacactttt taaaaaataa tcaaaatatt ttaaaaatta
     2821 aaagacgagg gagaatatta tgaaaaatat attaaaaaaa gtaggaatat ttacaattat
     2881 gttaggttta ttaggtggag ttgtaggttg ttcaaaacca gataatgaaa aagataagga
     2941 tgcttcaaag gaatctaaaa aagaagtagt tgtagggttt gataatacgt ttgtaccaat
     3001 gggattttta gatgaaaagg gagaaacagt aggatttgac gtagacttag ctaaagaaac
     3061 atttaaaaga cttggaatgg aagttaagtt tcagcctata gattggtcaa tgaaggaaac
     3121 tgaattaaat gattcaaaaa ctgtagatgt attatggaat ggatattcta taacagacga
     3181 aagaaagaaa atagtatcat atacagaacc atatttacaa aataagcaga taatagttac
     3241 tctaagtgac tcaaaaataa attcaaaagc tgatttaaaa gataaagaag ttggaacaca
     3301 acaaggttca acagcacttg atgcagtgga aaaggataaa gattttatga atagcttaaa
     3361 aggtggagca ccagttcttt atgatactta tgataaagca cttagagatt tagagatagg
     3421 tagaactagt gcagttgttg gtgatgaagt tttaattaga tactatatgg gacaaaaggg
     3481 tgaagacaaa tacaaggtat taaaagacga ttttggatta gaagattatg ttgtagcaac
     3541 ttctaaagaa aatccagaac tttgtgagaa aataaatgaa acattaaaag aaatgaaaaa
     3601 agatggaact tttgacaaaa tttatgataa gtggtttaaa tagaagataa gttagctgtt
     3661 aagaaggggg cactaaaggc atgttacaag gtttagagat agttatagca atgttttgta
     3721 taacattgat agtatcaatt ccattgggga ttggagttgc atttttaaga ttatcaaaga
     3781 ataaattagt aagtggtatt acacaatgtt atatattgat aatgagaggt acacctttac
     3841 ttttgcaaat gatagtcata ttttacggat tacctctttt aggaattgta tttgacagat
     3901 ttacagctgg agtagtagct ttcttcttaa attatgcagc atattttgca gaaatattta
     3961 gaggtggaat acagtcaatc gatagaggac aatatgaagc ttcaaaagta ctaggatttg
     4021 ataagtttac tatgtataag agagtaatct ttccacaagt ttttaaaaga atactagcac
     4081 ctatttcaaa tgaagtaatc acattagtaa aagatacatc tttagtatat atattaggat
     4141 taaatgatat tttaagaata tctcaaatag caatgaacag ggaagcaagt ctgttaccat
     4201 tatttgaggc aggagcgata tatcttatat ttgtagctat acttactaag ggatttgaat
     4261 tacttgaaaa aaaatattct tattatagat aaagttacaa tattatagga ggaggttttt
     4321 tatatgttga aaatcaaaaa cttaaataaa tcatttaaaa aaaatagagt acttaaagat
     4381 atttcttttg agttagaaga aggtcagatt ggagttttac ttggtaagtc tggtgcagga
     4441 aagaccacaa tattaaggtg tataaatggt cttgaagaat ttgatagtgg agagattata
     4501 atagataatg aggtaataaa aaataagaga gatatggcaa aaatacgagg aaaaataggt
     4561 atggtttttc aaaatttcaa cttatttcct catatgacag tccttgaaaa tataattgaa
     4621 tccccagtta atgtttttaa agttcctaga aaagaggctg aagagagggc tagagaactt
     4681 ttaagattag ttgatttaga agataaatta aattcttatc catttgaatt gtcaggagga
     4741 cagcaacaga gagttgctat agctaggtca tgtgcattga tgccaaaagt cctttgtttt
     4801 gatgaaccta cttctgcact tgatattgat actattcaaa gagtagtcaa tataatgaat
     4861 agacttaaag acaaaggtat gacaatatta ataattacac atgatgttgt cttctcaaat
     4921 aatgttgcag ataaaattat aagtataaaa gatggtatag tagaaaatgt tcaaataaaa
     4981 gaaaaaattg tttagagttt tataaatata aactagggta gaattttata atataaataa
     5041 agggcaagta aaatcaaata tactatgccc ttttgttatc tcaaaattta atcttatatt
     5101 ttatgtttaa gttacattat aatttataat aaaaataaaa ggactattta aatatataat
     5161 gtgaataaat gtttaataat ttgaaatatg ataatttttt atgttgaaag ctt
//
LOCUS       DAGSWI010000199         5151 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.203, whole genome shotgun sequence.
ACCESSION   DAGSWI010000199 DAGSWI010000000
VERSION     DAGSWI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.203"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            63..839
                     /locus_tag="MJF80_003642"
     CDS             63..839
                     /locus_tag="MJF80_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBZ0282249.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(931..1683)
                     /gene="xth"
                     /locus_tag="MJF80_003643"
     CDS             complement(931..1683)
                     /gene="xth"
                     /locus_tag="MJF80_003643"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBZ0282250.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(1844..2794)
                     /gene="galT"
                     /locus_tag="MJF80_003644"
     CDS             complement(1844..2794)
                     /gene="galT"
                     /locus_tag="MJF80_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBZ0282251.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            3179..4375
                     /locus_tag="MJF80_003645"
     CDS             3179..4375
                     /locus_tag="MJF80_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBZ0282252.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(4710..>5151)
                     /locus_tag="MJF80_003646"
     CDS             complement(4710..>5151)
                     /locus_tag="MJF80_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBZ0282253.1"
                     /translation="VKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRALVFI
                     KNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEEGDKI
                     ATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
BASE COUNT         1847 a          777 c          620 g         1907 t
ORIGIN      
        1 attttattta taatataatc tatagtaaaa gaaataataa aaaaatttag gagataaact
       61 ttatggaata cataattttc gatttggaat ttaaccaagg atttgataaa aaactcaata
      121 aaactgtatc caatgaaaag tgtccttttg aaattataca aataggagca attaaattgg
      181 attctaaatt caatataata gatacgttta atagctatgt aaaacctaca atttataaag
      241 acatacatcc ctttattagt cgtatgacta atattaaaaa ttctgatttt aatgattcgc
      301 ctactttccc tgaggtctac aacaatttta taaaatttat atcatcacaa gaccctatat
      361 tatgtgtttg gggagctgga gatttaaagg aattatacag gaatatcaac tatcacaagc
      421 taccatcaaa ttccttacct aaatcttata ttaatataca acaacatgca tctaaatatt
      481 tcaataatcc agcaggaaaa agcattggcc tacaaaatgc tatttctata ttagaacttg
      541 atgaaaaaat gtcttatcac aatgctttaa atgatgcata ttatactgct aaagttttta
      601 ttaagatata caatccaagt atagtcccag atatatatct ttatacgagt ataaaaccta
      661 aaactataag atattctaac aaaaaaagag ttgattatga taagttattt gatgaattta
      721 ggaaaatttt aaatcgtgaa ttaactaaag acgagaaaaa aatcattaac ttagcttata
      781 atatggggaa aacaaatcaa tttacattag aaaatgttaa gcaaagaaaa aacaaataga
      841 gtttatttag ataaaaaata ggcatgtaga ttaaaaaact agttaacttt aaatttatat
      901 ctacagccca atttttatca taattaattt ttattttatc tctaattcaa ctggacaatg
      961 gtctgaacct aatatttcag tgtgtatatc cgcacttact aatctatctt ctaatttttt
     1021 ggatgcacaa aaataatcta ttctccaccc tgcattattt tttcttgcat taaatctata
     1081 tgaccaccaa ctataaacac cttctttatc aggattaaaa tatctatatg tgtctataaa
     1141 cccactgtct aataatttag aaaacttctc tctttcttct ttagtaaaac ctgcattttt
     1201 catattattt ttaggatttt ttaaatctat ttctttatgt gcaacgttca tatctccaca
     1261 cacaattaca ggcttatgat tatctagttt ggttaaataa tctataaagt cgtcttccca
     1321 tctagttcta tattctagtc tctttaattc agattgagaa tttggcgtat atactgtaac
     1381 aaagtaaaaa tcttcaaact ctagagttat cactctaccc tctttgtcat gttcttctat
     1441 atttattcca tacattactt ttaaaggctc tttttttgta aatatagcag tacctgaata
     1501 accctttctc tcagcataat tccaatattg aaaatatcct ggtaaatcta actctatttg
     1561 cccttcttgc aatttagttt cttgtaaaca aaatatatct gcatctactt ctttgaaaaa
     1621 gtctaaaaat cctttcccaa cacaagctct tattccatta acattccatg atataaattt
     1681 catacttata ctcctcatct aaacattgac acactcccca tagctaaaga tgggggcttt
     1741 tcaacaattt tattgtaaat tcttaattca cttttatttt aacaatgcta tactataatt
     1801 tcatctaaaa ttactattta cttaatacct aaaaatatat gttttaatca atttttaact
     1861 tttttgttaa atcttcaggg ctcattgaag aaacatacat atttgttcca agttcaaatc
     1921 ctccaaaatt atattttctt gggattatgt gcatatgaac attaaaatac gattttgttt
     1981 caatctttgt tggatgagtg tgaatacata agttaaatgg catatatcca ttgatattgt
     2041 ataacttctt aaacaacttt tcaaatattg tagaaagctc ctgtatatta ttatcattta
     2101 actcaccaaa ctctatttta tctttaaata ttattcttac ttctccacta tacttggttg
     2161 catatggtat aagtaccaaa aaagcttctc cattataaac aactcttcta tctaaactta
     2221 tttcattttc tattaaatca tcatacaaag agcttttatt gctgtcataa tactcctttg
     2281 aaaccaacag ttcattcgta atttcaggtg gaataaaaga catagacaat atttgagcat
     2341 gtggatgcat caaagatgct cctgcctttc tcaagaagtt tttgaagata ctaacatact
     2401 ctactttatc atctttcgac aaatctctaa atctatttct atacataaaa aaaacatctt
     2461 caaattcttt ttgactcatg ttataaaaac tacgattatg tttatcgctc tctatcatta
     2521 cttcatgaac accatataca tctcttgcca tatcatcaat tataggaaat ttattgtaga
     2581 cagattttac aagccatcct tcttcactat ctatttcaaa taatgcatca gttgcatgag
     2641 cttcatttcc tttgcaaaat gggcattctt catcatattc attagtttct tcatcttcat
     2701 ttgggatatc tgctgtatct aaaggtcttt ttaacctatc tgtagcaaat ataacaacat
     2761 cattggtaat tggatctatt cttaattctt tcataattaa cacctcttag ttaatagcct
     2821 ttaatataat tatatgatat ttatcgtatc tgttaaataa atttataata ttttttataa
     2881 ttttgcatat ttattattat atattttgtc tatagctgaa agattataac taaaaaatca
     2941 ctatcagtac atcagaatat tatcttacta taaattaatt tatgaataca taatttagtg
     3001 gcataaacat gggtatttta ctataatttg ttaaaagttt attttaaagc tatattaatt
     3061 ttttttgatg atttttaccc ataagtatgt tattattatc ttgtaaagat gtcttgtatt
     3121 caaacataca atttacataa tacaatatac gtatttaaat taaatagggg gtattaaaat
     3181 gaattattca aacagagtta gtgcaatgca agcctctcca atcagaaaac ttgttccatt
     3241 tgcacaagca gcaaaagata aaggaatcaa agtatatcac ttaaacatag gacaaccaga
     3301 cataaaaact cctaaaggat tttttgatgc tgttaaaaac tttgatagcg aagtcttaga
     3361 atatgctaca tcagaaggta ttccagaatt attagaagca cttcaaaatt actataaaac
     3421 ttacgatatg aattttgaaa aagatgaatt attagtaact aatggtggta gtgaagcttt
     3481 acttttcact atgatggcag tttgtgaccc aggagataat ctattagttc cagaaccatt
     3541 ctatacaaac tacaatggat ttggtcaatc tgtaaacgta gaagttaatg ctgttacaac
     3601 aaaggcagaa aacggtttcc accttccatc taaagaagaa atattgtcta aagtagatga
     3661 taaaactaag gctataattc tttctaaccc aggaaatcca actggtgcaa tttatacaaa
     3721 agaagaactt aatatcctag ctgaaatagc taaagaaaaa gatttatgga taatagcaga
     3781 tgaagtttat agagaatttg tttatgatgg gcttgagtat acaagctgtg gtaacttaga
     3841 gggtgtacag gatagagtta taataataga cagtgtttct aagagataca gtgcttgtgg
     3901 tgctagaatt ggttctatcg cttgcaaaaa taaaggtctt atcgctcaaa tacttaaatt
     3961 atgtcaagga agactttgtg ttcctacatt agagcaaata ggagctgttg agttatacaa
     4021 aactcctgtt tcttacttca aagaagttaa tgaagagtac aaaaaaagaa gagatgtatt
     4081 atacaatgaa ttaatgaagg ttgaaggtgt tatatgtaaa aaaccaactg gcgctttcta
     4141 tatagttgct aaattaccag ttgaaaatgc agaagatttc acaatttgga tgttaaaaga
     4201 atttaacaaa gataatgaaa cagttatggt ttgccctgct gaaggattct atgctactcc
     4261 aggtttagga cgtgatgaaa taagattagc ttacatctta aatgaaaaag accttcatag
     4321 agctgctaca ttattaaaag aaggtttaga gcaatatgta gctttacaaa aataattttt
     4381 taaacttaat ttatataaat ttaatgatat aaaatgtccc cttttcagat attatttgaa
     4441 aaggggacat ttcttaatta atatattaat taagacttag acctgactat ttttcatttt
     4501 ctttatatag tgttggccag taatctttat tcactttaat taaagcatct aaaacttgtc
     4561 ttgccttagt tagagaaatt ataaaaaagt acatcaagaa aatttaagat gtacttttta
     4621 gattgtattt agagttataa ttatatagtt aaatgaataa caaagaatta atcttagtac
     4681 cattaaatac aaaaattttt atattttttc tatctaaatt cttctttatt taatatatat
     4741 tgaagagtat ttattgcttc taaatatttt tttctgtctt cttcagttaa tttagaaagt
     4801 ttttctccta aaccatcact taatttatcc ataaaatcag ttgctatctt atctccctct
     4861 tcagtaatgt caattagata ttttcttcta tcaactaaat ctcttttcct tgttacatac
     4921 ttcttatcag ttaaatcatc aaccatacta gttaaactgc ctttttctat atttaactta
     4981 gagcataaat cagtcatact tattactcca taatttttta tgaaaactaa tgctcttaat
     5041 tgtgttttat ttacattata atctggtgca aattttttta ggtcttctaa gtacaggcta
     5101 gaataaatct taggaaatgt ttttactaaa aaacttatgg tatcttttac t
//
LOCUS       DAGSWI010000200         5086 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.205, whole genome shotgun sequence.
ACCESSION   DAGSWI010000200 DAGSWI010000000
VERSION     DAGSWI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.205"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(249..1841)
                     /gene="hcp"
                     /locus_tag="MJF80_003647"
                     /gene_synonym="priS"
     CDS             complement(249..1841)
                     /gene="hcp"
                     /locus_tag="MJF80_003647"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBZ0282254.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(1901..2626)
                     /locus_tag="MJF80_003648"
     CDS             complement(1901..2626)
                     /locus_tag="MJF80_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBZ0282255.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            2791..3495
                     /locus_tag="MJF80_003649"
     CDS             2791..3495
                     /locus_tag="MJF80_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBZ0282256.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFKK"
     gene            complement(3659..4930)
                     /locus_tag="MJF80_003650"
                     /pseudo
     CDS             complement(3659..4930)
                     /locus_tag="MJF80_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         1673 a          894 c          619 g         1900 t
ORIGIN      
        1 ttcaaacctt taatacttaa aataaaatat ataagatatt atatatctaa gtattataaa
       61 tttaatacaa agattcttaa aaaaaatata ctgaaagcag gtttattttt caattattac
      121 aaaaaataaa taaagggtca tctcaaaatt gattttttaa tcaaatttta agtttaaccc
      181 cttatcttaa ttgtggcata cttaaaaata gtttaactat aaatatcttt aagtatagat
      241 atatttattt atcctaaaat cttcttcata tcttcttcag gagtagatat aggtgtcaat
      301 gcaaatttat caaccaatat atctaataca ttcttagata agaatgctgg taaagaaggt
      361 cctatataga tatttttgat acctaaatga agtagtgtaa gaagtataca aacagctttc
      421 tgctcatacc atgacagtac catgctaaga ggcaaatcat ttactccaca ttcaaaagca
      481 tttgccagtg aaactgccac tttaattgca ctataagcat cattacattg tcccatatcc
      541 attaaacgag gtaagccacc tatatcccca aggtctaaat cattaaatct atattttcca
      601 catgcaagtg tcaatataac agtgtcttta ggagcttgtt ttacaaattc agtgtaataa
      661 tttcttccag gttttgcacc atcacaacct ccaaccaata aaaagtgtct tatagctcca
      721 ctctttacag catctactac tttatcttgt acagatgaaa ctgtacctct tgcaaaacca
      781 gtagttaatg tatctcctcc attaatacca gtcatatgaa catcttctct atatcctcca
      841 agttcaagag ctttttctat tacactagaa aaatctttat cttcatcaat atgaaccata
      901 cctggataag caactacttc tgtagtaaat actctatctc tataactatc tttaacaggc
      961 attaaacagt ttgttgtaaa taatattgga gcaggaatat tatcaaattc tttttgctgg
     1021 ttttgccatg ctgtaccaaa attacctttt agatgtttat attcttttag ttttggatat
     1081 gcatgtgctg gtaataactc accatgagta tatatattta tacccttatc tttcgtttgt
     1141 tctaataatt tgtgtaaatc ataaaggtca tgtcctgtaa ctacaataaa tggccctttc
     1201 tcgattttca tagatacctg tgtaggttct ggtgttccat aagtttcagt atttgctcta
     1261 tcaagcaatt ccatacattt taagtttatt tctccagttt tcataactag acctaaaagt
     1321 tcgtcaatag acaagtctcc tccaactgct attaatcctt catagaaaaa ttcatttacg
     1381 tctttgtctg tataaccaag taccattgca tgatatgcat aggcagccat acctcttaat
     1441 ccaaataaga ttaatgattt taatgaacgt atgtcttcat tatcattcca taaatccttc
     1501 atatcatagt tatcatttct attacaagtt gttgaacaat ctgaacatct aggtacaagt
     1561 ttactttttt ctacctcagt tttttcaatt tgtcttttta tagcttcatc atcaaaactt
     1621 acattcgtta tagtagtgaa tagcccctct atcataattt tatctgtttc acttgttggt
     1681 ctattcttat ttccaatagt tgctcttgaa agaccaatca acactcctgt aagttcgtct
     1741 tgtaagttag ctgtaccccc ctttttacca cagacaccat ttatagtaca acctttacct
     1801 cctgctgttt gttcacattg aaaacaaaac attttttcca ttttttattc ctcccttttt
     1861 atttattaaa atttattata tttttctata attcacttaa ttaaatttct ccatctacaa
     1921 tttttccatc tacagatata gtaactattt gccatggtat catttttcca ctattttgta
     1981 gagctgtctt aacagcattt actataccac cacagcaagg aacttccata cgtgttacta
     2041 ctatattttt aatatcattt tcttttaaaa tctctgtaag tttttcagca tagtcaactt
     2101 catcaagttt agggcatccg attaatgtaa ccttattttt cataaattta ttatgaaaat
     2161 ttccatatgc atacgctgta cagtctgctg ctattaaaag acttgcattc tttaaatatg
     2221 atgcattagt tggtactaat tttatttgta ctggccattg attaagttga gaacctttta
     2281 tctctactat atcattgctt atagacgtac tatttgaaac ctctctattt attgacttag
     2341 actgagaacc tggacatcca catgctaaag ttttcttttg tgctttcttt gcttccatat
     2401 ttgcttttac tgcagcttca tcatattctg ctgcttctct atactcaaat gaaatcgcat
     2461 ttgttggaca agttggcaga caatctccca gcccatcaca atagtcatct cttaaaagtt
     2521 ttgcttttcc atttaccatt cctatagctt cttcatgaca agcttcaaca catagtccac
     2581 aaccattaca cttttcctca tcaattttaa ttatttttct catcatttat atcacctcat
     2641 aaaatttttt taatttaaag tattgatttc ttatatattt atttttaata cctatatttc
     2701 tttatttcaa atttcttgta gtaggatttt tatatcccct tgcttttata aacttagtat
     2761 agtacaatga aaacaaatta tcggttgcat atgcaacaag gaagtgatta tttgaaaaaa
     2821 tttttagaaa agcatataga cctacttaaa aaaattcctc tatttgaagg aattaaaagt
     2881 gatgaacttg aagaaatgtt ggaatgttta ggtgttgtag ataagactta cccaaaagat
     2941 agtataatat tctctgttgg aagtgaagtt acttctattg gaattatgct taaaggaagt
     3001 gctcatataa taaaggaaga tatcgaaggg aataggaata ttgtagctga actcttgcct
     3061 ggagatttat ttggagaggt attcgcatgt actagactac ataaaagtaa tgttacagta
     3121 accacaacat cttcttgcga agttttattt ataaagttta aaagtgtaac tggtatttgt
     3181 tcatctgcat gtgtatttca taataggctt gttgaaaata tgctacaatt aatagctgaa
     3241 aaaaacattt tacttcacaa taaaatagaa ttactttctc gtaaaacaac tcgcgagaaa
     3301 ttgcttatgt atttttcaaa acagatagaa caaacaggta gccaccagtt tacaattccc
     3361 ttttcaagaa atgaaatggc tgatttttta tgtgttgaca gaagttctat gtcaagagag
     3421 cttggcaaga tgcgagatga aggcttgtta tcttttaaca aaaataaatt tgaaatcttt
     3481 tattttaaaa aataaaatag atatttaaac atatttattt caatatatac tatattagtt
     3541 gattcaaatt ataaatatca tatttgaatc ttaaatttat atttttactt aaaaagttaa
     3601 taggtatcta atttattaaa attaagatac ctattaactt tattaattaa gtgtacaatt
     3661 agcttatttt ctttgtagta attgctgttt tatctataag ccaatctttt ccttctacta
     3721 atcttgaaac taacattgat gatactgtat ttccagttgt atttaatata gtattaggaa
     3781 tatcagttat agtagttata actgttaaca gtggaattgc ctccactgga aaaccgaaga
     3841 tgctaaccac gagcatctca ccaataagtc agccacctgc tacaggagcc ataaatactc
     3901 caacaacaaa tacagttata aataattgac ctatcgttgc tggatttgcc attccactac
     3961 ctaaaatacc tgataaaaat gccactttta ttattactgc taaaactgac ccatctttat
     4021 gtatatttat tccaagaggt ataactgttt ctgatatatc atcaggaact cctatatttt
     4081 tactagcttc taaatttgat ggtatacatg ctgaacttga agaagtagct agtgccgttg
     4141 ctattggttt tatagcattt ctccagaatt gtctaaaccc ttgttttcct gcagataaaa
     4201 aagcatacag actgtaaaat aataagaaat acatggctgc tgttagtgaa taaattacaa
     4261 acacccttac atatccttga attattttac ttccaagttc cccaattatt gtagctgtat
     4321 aacagcctag ccctatagga gcataaagca tgaatatact tactactttt aatattgctt
     4381 ccgttccttc tgataaaagc tttcttattg tatttcccct atctcccatc atagctattg
     4441 ctgttccaat taaaactgca aatataacta attgtagtaa attactactg gaaaataaat
     4501 ctccaaaatt tttaactgta atacttgata ctacttggtc taataacgtt ccttttgatt
     4561 ctaaagtttc tgcatcttga gccaccatta aattttttac acttgataca tctaatcctt
     4621 tagacggatt aaataataat gcagatgtaa tacctaaaaa tgcaattaca agtgttgttc
     4681 ccataaatac tatagctgta cccttcatta tttttccaaa tcgttccata ttttttatac
     4741 tagcaacacc tgaagaaaca ctaagaaaaa ctaatggtac tactatcatt aatagagcat
     4801 ttatgaataa atctccaaat ggttttagta ttgaagcttt tggaccaagt actaatccta
     4861 ctatcgcccc tactatagta catgctaaaa gtataattga tgacttataa ttttttaaaa
     4921 agtccctcat ttatattttc ctttctaatg ctttatatta taaattaccc caaatgctaa
     4981 aaaaattaaa gcaaattatc taatgcgcat taataatttt tgtaattttc cccaacagct
     5041 actttagcaa atactggaat atatgtcaat ttctatagtt tttttg
//
LOCUS       DAGSWI010000201         5020 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.206, whole genome shotgun sequence.
ACCESSION   DAGSWI010000201 DAGSWI010000000
VERSION     DAGSWI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.206"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            169..816
                     /locus_tag="MJF80_003651"
     CDS             169..816
                     /locus_tag="MJF80_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBZ0282257.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNKALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(983..1552)
                     /locus_tag="MJF80_003652"
     CDS             complement(983..1552)
                     /locus_tag="MJF80_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBZ0282258.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1557..2456)
                     /locus_tag="MJF80_003653"
     CDS             complement(1557..2456)
                     /locus_tag="MJF80_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBZ0282259.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2467..2616)
                     /locus_tag="MJF80_003654"
     CDS             complement(2467..2616)
                     /locus_tag="MJF80_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282260.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2609..3193)
                     /locus_tag="MJF80_003655"
     CDS             complement(2609..3193)
                     /locus_tag="MJF80_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBZ0282261.1"
                     /translation="MKRFFIVVLGCIFVLGVISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            3582..4907
                     /gene="cdeA"
                     /locus_tag="MJF80_003656"
     CDS             3582..4907
                     /gene="cdeA"
                     /locus_tag="MJF80_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBZ0282262.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIIALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKGRYKY"
BASE COUNT         1731 a          765 c          609 g         1915 t
ORIGIN      
        1 gtaaatctta tactaatcgc atatttatgt tataactata ttgtaactat tttcgtaatc
       61 ttaggaaaag attacttata aaattaacct tatacaataa tatatcgtat aatcttaatt
      121 agcataatag ttacaatatt aaaatttaaa tatgggagtg attttagtat gataagacaa
      181 acaccattag agaaacgata tgacaagcta ggtcctcata ttgtaacagc actcaaaaaa
      241 cgttattttg atgcttatta ctgtaaaact agaaataaag ctttgcaaca aattttagat
      301 ttaattcctc aaaatcatgt agtatcatgg ggaggatctg aaacacttaa ggaaatgggc
      361 gtacaaactg ctgttagaga gaaaggattt aaagttattg atagagattt agcaaaaact
      421 ccagaagaaa gaactgaaat tatgcgtcaa gcacttctat gtgatacttt tttgatgagt
      481 agcaatgcaa ttagtgaaga tggacaactc tttaatattg atggaaatgg caatcgtgta
      541 gctgcaatga tatttggtcc aaaaaatgtt atagttgtag ctggtatgaa taaaattgta
      601 aaaaacattg atgatgcaat acaaagaact agaacaattg catctccagc agttgttcag
      661 cgttttcaag atgtcaatac accatgctat attaatggaa gttgtataga ttgcacaagc
      721 cctgaaagca tttgtgcata catggttact acaagaatca gtcgtccagc aaaaaaaata
      781 aaggtaatat tggtaggaga aaatcttggt ctataaaata tacatacatg taaatagctc
      841 tataaataat aaaatcatat gaaataactt atttctaaaa gtggtagaat aaaagcttct
      901 acaagacttt cattctacca cttttatatt cttttaattt gtgtttctat attattaatt
      961 gctccgattt tcatatatat tactaactat ttttattttc ataatttttt tgttcttcat
     1021 caagctgttt ataataatct ttattatagt cagctagata atctatacca cctcctgtaa
     1081 tcacatttaa taatttcatt gcagattctt gattaacttt acaaacataa ttatcaccag
     1141 atttaacaag ctctaatgta atattaaatt cttttttctt tgcagaatca atatctttca
     1201 tagttggatt tggcatcatt tctcctagtt taggaggatt cttttttaaa atagaaacca
     1261 tatcagtatt gttaattttt aaatctacag tcactttatc cttattattc tttatatctg
     1321 aaatttccca agtcatattt ttagaataca ttttaaatcc ttctttatct tcgtatataa
     1381 gattacctaa ccctgatttt gggaaatact tatcaacagt atctgcatct tgtgttttta
     1441 ttgaattaaa catactatcc acttcatttt ttatcttatt atcattagta caaccagcta
     1501 acaccagtac tgatattgca aacattataa tacatataat aaatttcttc attaaattat
     1561 atccccctat acttttttgc acaaatatta ttctcttttt tatcttttga aaaagttgta
     1621 gtaattgcat tttgtggaca aacttttata cattcaccac acctaataca gtctggacta
     1681 tttacatctt tatatattgg tatatcaagc ttacattttg tttgacatgc tgtacaattt
     1741 gtacatttat ctttatcaat tttaaatcta tatattgaaa taggattaaa aaaaccatag
     1801 attgctccta aagggcatat atacttgcaa aatggcctat aagtaatgat tgacatcaat
     1861 aatatcaata gcaaaatact taacttccag ctaaataaaa atcctattga ttctcttaaa
     1921 ttctcatttc ctaaaataag tggtattcct cccaaaagtg ttcctgaagg gcaaatccat
     1981 ttacaaaacc aaggatttcc accccctgct acatttacaa tagcaactgg caaaataatt
     2041 acaaacatta ttaagactat atactttaga tattttagat atttatcacc ataaactttt
     2101 tttatcttta ctggaaaggg tactttatgt agtaaatctt gtacaagtcc aaagggacaa
     2161 agccaaccac aaacaaatct gccaaaaaaa gcaccaaatg ccattaaaaa accaacaaca
     2221 taaaatgaga atttgtactc tctgcttgat aaaactgctt gtaatgaacc tattggacat
     2281 gctccaagtg cacctggaca tgaataacaa ttcaatccag gtacacataa ttttttactt
     2341 tctcctccat atatagttcc ttctaaaaat ccatttacat atccatttgt taatgctgta
     2401 aatgcaactt gagtaataag tcttatatta ttatttacct tagataataa cttcatccct
     2461 cttctcctaa ccaataccaa tacactcaag acaaatcata attgctttag ttaatactgt
     2521 atttgtttct ccacgatatg tacctaatgc aataaatata atactaattg caataataga
     2581 aatgcctttt ttattatctt taataaagtt actcattggc ttttaccatt tttagtcttt
     2641 catctataat ttttttccaa tcttccttac tatttgcacc tacaaccttc tcacccacaa
     2701 agtttccttt cttgtctaca aaataagttg ttggaaactc cataactctt ttaaaatcag
     2761 tttttttaag ttcttcagat acagtcaatt gttggtaagt tgcatctgac tttttcataa
     2821 tgtctttaac taaatttttc tcttcatttg aaagaccagt tctcatatca gttttatcaa
     2881 cttctacaac taatcccttt atagctacat tggaattatt actctcatat tcttttgata
     2941 atgcttcaag ctctggcatt tcacccacac atggtccaca ccaagtagtc cagacattaa
     3001 taagagtaag ttctttattt gaaaatatct cttttgttac cttatttcca tcaacatcct
     3061 ccgtatctaa attactaaac cattcactat tattcttgtt tttttctact ggcttagatt
     3121 cttttttgct acatccagaa atcacaccta aaacaaaaat acatcctaaa actactatga
     3181 aaaacctttt catatctcac actcctagaa tttatatagt atataaaatc gctgcttgaa
     3241 ataaaaataa cttatctact atattaaagt aattatattt aataaataag agctaattta
     3301 aaaaaagata tacagttaaa atctatttct tttcagacaa aatcttatat atatataata
     3361 gcttgtaaat cttaagatta tatacatata tttctaaaga ttttcttaag aaacagttag
     3421 attttactaa tataagttta ttaattagca ttatactaat ttatcttaat atgatttttt
     3481 gttgatttta agctgtttat cgtataaata aaatttttct tgactatata gttactatat
     3541 agcttattat atatagagag taataaaagg aggtaaaatt tatggaaaat ttatttacaa
     3601 gaaaattcac tacttttgaa tttctaaaat ttgtttctcc tgcaattata tccatgatat
     3661 ttatatcttt gtacacaata atagatggca tctttgtatc gacattagtt ggttctgatg
     3721 ctcttgctag tataaatatt gtactaccta taattaacct tgtttgtgga tttggaataa
     3781 tgatggcaac tggtggaggt gctatcgttt ctatacgtat gggtgaaaat agacaggatg
     3841 aagccaactc tacattttct tttatagttt tgttttcatt gattgttggg attttattca
     3901 cagtaatctc atatttcttt atcaaagaaa tatctatttt gcttggtgca acagataagt
     3961 tattaccata ttgtataact tatggtaaag ttatgatttt atgtacacca ttttatattt
     4021 taaaatttat atttgagtac tttgcaagaa ctgatggaaa ttctaaattt agtttatttc
     4081 tatcagtcat tggtggtgta acaaatataa tattggatta tgtatttatt aaatattttg
     4141 gaatgggtct tttaggagct gcagttgcaa ctgctatagg tattatttta acttgtgttt
     4201 taggtattat ttacttctta tctaataaat ctacactaaa attaagaaaa ccaaaaaccg
     4261 attttagact tataagagat actatgataa acggttcttc tgaaatggtt acagaattat
     4321 ctacaggaat tacaacattc ttatttaatg tagtagcttt aaaattagca ggagaaaatg
     4381 gacttgctgc tcttactata gtattgtatg ctcatttttt aatgacatca gtctatctag
     4441 gattcgctgc tggagtgtct ccattaataa gctataattt tggtgctgaa aacagtgata
     4501 aattaaaaga aacatttaaa cattctctaa aatttatatt tgtttcttct cttttagtgt
     4561 ttattattgc tttagtattt gcaccattta ttgttagggt ctttgtaagt ccagataaca
     4621 cagtatttaa actagcctta caaggattaa aaatatttgc atttgctttt ttgtttgttg
     4681 gtataaatat atttgcatca ggatttttta cagcatttca caatggaaaa atttcagcta
     4741 ttatatcttt tagtcgtgcc tttgttttta taatcatagg aatcataatt cttcctccta
     4801 tgttaaacat gactggatta tggcttacag ttccatttgc tgaggttata accatattta
     4861 tatctattct atttataaaa aaatataaag gtagatataa gtattaaaaa ttaatactta
     4921 aaatcaacat ttattaaaac taataaacct gctttaaaat acaaatttac aaatttgatt
     4981 tttaagcagg ttttatttta gttataataa gtcatttgca
//
LOCUS       DAGSWI010000202         4976 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.207, whole genome shotgun sequence.
ACCESSION   DAGSWI010000202 DAGSWI010000000
VERSION     DAGSWI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.207"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     misc_binding    250..481
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            589..2070
                     /locus_tag="MJF80_003657"
     CDS             589..2070
                     /locus_tag="MJF80_003657"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBZ0282263.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISSDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2080..2973
                     /locus_tag="MJF80_003658"
     CDS             2080..2973
                     /locus_tag="MJF80_003658"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBZ0282264.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3097..4158
                     /locus_tag="MJF80_003659"
     CDS             3097..4158
                     /locus_tag="MJF80_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBZ0282265.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4313..4825)
                     /locus_tag="MJF80_003660"
     CDS             complement(4313..4825)
                     /locus_tag="MJF80_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBZ0282266.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         1856 a          609 c          768 g         1743 t
ORIGIN      
        1 tagtgtagta taaaattgta atattacaga tatttaatag ttatcttttg attactttca
       61 ttaaaaacag cttcatcact atgaaaatct aggtttttaa acttttaaaa aagttaaaaa
      121 aatatttttc taatactcaa tttgattaca atttttttac aatttaaaaa atattttttc
      181 caaattttta tattaatact tgactttgtc tgttttataa actagaataa ttaataatat
      241 taagaatatt gaaaggcatt gattaggaag agtaaatctg aatcgattac cagagaagga
      301 aactactagc tgaaatgttt ccctaattag aaggattgaa aactacctag gagcttctat
      361 ccgaagttat cttaatgatt ttagtaggaa tagacgtttt tattacttgc gttaaagtat
      421 caagtggatt ttttaaaatc aatttgggtg gaaccgcggg aaacagattc tcgtcccttt
      481 tttagggatg agggtctttt tttattacct aaattcagtt tttaaaatat tctgtatcta
      541 atttaattag ctaattaaaa atttaaaata tatggaggat tataaaaaat gtattattca
      601 agtacgagag gcaccgaaga aaaagtaact gcatcacaag caataataaa aggaatatca
      661 agtgatggtg gtctttatgt tccaagtgaa tttcccaatg taaaaaatga acttataaat
      721 ttagtaaatt taacgtattc tcagatagca ttttttgtac ttagtaaatt tttatgtgat
      781 tttactgaag atgaaattaa aaattgtata gaaaatgcat atgatgaaaa gtttgattgt
      841 agtagtatag ctccattaaa taaagtaaat gatacttatt tcttagaact atatcacggt
      901 ccaactttag catttaaaga tatggcactt actataatgc ctcatcttct taaaacatct
      961 ataaagaaag ataatttaga gaaagatgtt gtgattctta ctgctacatc tggagataca
     1021 ggtaaagctg cacttgaggg atttaaagat atagataaaa taaagataat tgtattcttc
     1081 ccagaagatg gtgtaagccc agtacaaaaa cttcaaatga aaactcaaac tggaaaaaat
     1141 acatatgtag ttggtataaa aggaaatttt gatgatgcac aatcaggtgt aaagaagatt
     1201 ttttctgata aagaatttaa tcaaaaacta ttggataata attatatatt atcttctgca
     1261 aattcaatta atataggaag acttcttcca caagttgcat actattttta ttcatatatg
     1321 aaattagtta gcaatagtga tataaattta aatgataaaa taaattttgt tgtcccaact
     1381 ggtaattttg gtaatattct agctggatat tatgccaaac aaatgggtct tccaataaac
     1441 aaattgatat gtgcatcaaa tgataataat gttctttatg atttctttaa aactggtgta
     1501 tatgataaaa atagaacatt gaaattaact acttctcctt ctatggatat attaatttca
     1561 agtaatttag aacgattact gtttgaaata tcaaatagaa atactgatat tgtaaataag
     1621 cttttatcag accttagtga taaaggagtc tacaaaataa gtgatgatat ggaaaaaaat
     1681 ttagcctcat tctatggaga atattcaaat gaagaagaag ttaaaagcac gataaacaat
     1741 gtgtttaaaa attataatta tttgatagat actcatactg ctgttgcata caattgttat
     1801 gaaaaatata aaaaagaaac ttctgataac acaaaaactg ttattgtaag tactgcaagt
     1861 ccattcaagt tttctgaaga tgttctaaag tctatagatt gtgattttaa agaccttgat
     1921 gactttgcta taattgatag attatcaagt atttctaaaa tagatattcc aaaacctatt
     1981 aaaaatctta aaaatgcaga aatactacat aaagatattt atgaaaaaga tgaattaaaa
     2041 ctagctataa aaaaattctt aaaggtatag gtgattatta tgttagagat tatagttcct
     2101 gcaacaagtg ctaatatagg tccaggattt gattgtcttg gaattgcgtt aaatatatac
     2161 aataaatttt atgttgaaga aattgaaagt ggtcttgaaa tagaaggttg tgaagatgct
     2221 tacaaaaacg aaaacaactt agtttataca tctatgaagt acttttttga tagagttaaa
     2281 ccagaaaaaa tacctgctgg tatcaaaata aaaatccaaa gtgaagtacc tatatgtaga
     2341 ggtcttggca gtagtgcctc ttgtatagta gctggagtta tagctgcaaa tgctctatca
     2401 ggtgctaatt tagataaaaa tcaattacta aatatagctt cagaaataga aggtcaccca
     2461 gataatgttg ctcctgctat tttaggaaat atgattgtat ctgttactga taatgagaat
     2521 atacattatg atattataaa aattcctgaa gaacttaaat tttgtgcaat gatacctaat
     2581 tttaagttat caacagaaaa agctagaggt gttttaccaa aggaaatacc atattctgat
     2641 ggggtattca atgttagtag agttgcttta ctaatatctg ccttactaaa caaaaacttt
     2701 gacctactta aagtagcttg tcaagacaaa ttgcatcaag attatagagg aactctaatt
     2761 gagaactaca atgatattgt tgaaaaatct gaacaattaa atagtattgg agtattttta
     2821 agtggagctg gacctactat aatgtcttta ataaaagaaa atgatgatag ttttgtggat
     2881 aatatgaaaa attacctaca aaaactaaaa tctgattggg aaataaaaga gttatgttgc
     2941 gattctaatg gagctgtttt aaatataata taattatcgt cttctataca aaatataact
     3001 actcttttat tataatttgt agtataataa ttaggactag gtaataatat taagtattat
     3061 aatctagttc tttaaacatg tttacattga ggagggatta tcataaaaga gttaataatt
     3121 agagatttag ttgcaaaagt tcctataatt caaggtggaa tgggcgttgg tatatctagg
     3181 tcatcactag ctggtgcagt tgctaagctt ggtggtgttg gtgtaatttc tggagttcaa
     3241 attggatatg atgaggaaga ttttgaaact aatactataa atgcaaattt aagagctata
     3301 aaaaaacata tttctaaggc caaagaaatc tcaaatggtg gtattattgg tattaatttt
     3361 atggtagcta tgaaagaata tgaaacatac gtaaaggaag ctgttaaagc tggtgtagac
     3421 cttataatat ctggagctgg attacctaat aagctaccat cattagtaaa gggaagtaat
     3481 gtaaaaatag cacccatagt ttcaactgca aaagctgcta atgtaatttt aaaaatgtgg
     3541 gatagaaaag agaaaactac tgctgactta atagttgttg aaggtccaaa agctggtggt
     3601 caccttggat actctaacga ggaacttgat aacatagatt ctatagacta tgataaggaa
     3661 tttgttgaaa ttttaaaggt tgcaaatact tatggagaaa aatttggtag aaacatacca
     3721 gtagttgctg ctggtggtat aacttctagt agtgatgtta aaaaatatat agatatgggt
     3781 gcaagtggtg tacaagttgg tacaagattt gttgctactt acgaatgtga tgcccatgag
     3841 aactttaaaa tggcatacat aaatgctagt gaagaagatg ttcatatagt taaaagtcct
     3901 gtaggtcttc ctggacgcgc cataagaaac aaatttatag aagaagttaa aataaatcgt
     3961 ccagaaataa agaagtgcta taattgtttg attccttgta atccaaaaga aactccttac
     4021 tgtatttctc aagctctaat caatgcagtt aaaggagatg ttgaaaattc acttttattt
     4081 tgtggaaatg atgcctataa aattgataaa ttaagtactg ttgaagatgt tatcaatgaa
     4141 ttgatttctg aattataatt taaagaaaac taaattaaaa ttttatcaag ctaaggagtt
     4201 taaatacgtt atttattaat ctttataaac atatttatgt ccttagcttt tttgtgtcaa
     4261 tttcctttta tggaaatttt aatttaatta gagatagtat taacctttat aactaatctt
     4321 cttttacaag tacattggtt atttcagcta tatataaagt atgataatct ttttgtggat
     4381 accatcttcc atctatacct tcagcaataa aacattgagg ttcaagtttc tgactataca
     4441 tttttttgca tataatagcc attttagatt ctttaaaata aggtgtatca tttaagtgtt
     4501 ctatattaaa tccaatctgt gatatcttat cctcttctct accagatact tttccacaat
     4561 atgaaagctc tttacgaaaa tcttcatcaa aaaatgtaag agaaaattta tctgtattat
     4621 ctataaattc cttagtataa cgctgtggtc ttattactac aaaagcaaca tcttttcccc
     4681 acataactcc aagccctccc cagctagcag tcattgtatt cactttacct tcattttctg
     4741 ctgttataag tgtccaatct ttacctatca atccaaatgg attattttta atctcttctg
     4801 ccaaaatttc tttaaaatca ctcatatctc taataacctc tcattctctt atatttatat
     4861 tccttttatt ttcatgataa attataaatg aagtctagtt ttgaaaataa aatacttttt
     4921 taaatatatt attgcaaata agtaaatata tatatacact cttatttttt aatata
//
LOCUS       DAGSWI010000203         4884 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.208, whole genome shotgun sequence.
ACCESSION   DAGSWI010000203 DAGSWI010000000
VERSION     DAGSWI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.208"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            120..791
                     /locus_tag="MJF80_003661"
     CDS             120..791
                     /locus_tag="MJF80_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282267.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            793..1701
                     /locus_tag="MJF80_003662"
     CDS             793..1701
                     /locus_tag="MJF80_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0282268.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1778..2464
                     /locus_tag="MJF80_003663"
     CDS             1778..2464
                     /locus_tag="MJF80_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282269.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGELL"
     gene            2461..>4884
                     /locus_tag="MJF80_003664"
     CDS             2461..>4884
                     /locus_tag="MJF80_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBZ0282270.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWSLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINP"
BASE COUNT         2001 a          502 c          808 g         1573 t
ORIGIN      
        1 ttattttgaa aacaaattta aatacaatct ttttagtgaa ataaataaaa ttaaaatatt
       61 tttagtatga taatgcaaga aaataattat ttatctaaaa aatctaaagg agagtaaaat
      121 tgaacttatt aattattgaa gatgatataa acttaaatga aggtcttttt tatgcatttg
      181 agaatgatgg atttaatgta tttaaggctt atacaaaaca agaaggatta aatatattta
      241 atagtaaaaa tatagatttt ataatcttag actgcaatct tccagatgga gatggatttg
      301 atgtatgtca aataataagg gaaaagtcag atataccaat tataatgctt actgcaagag
      361 actcagaaat tgatgaagta aaaggattag aaatagggct tgatgattac attacaaaac
      421 cattttcttt atctgtatta aaggcaagag tcaaagtagc aataagaaaa aaatcgaata
      481 ataaagtaat atattccaat ggaataaaat tggaccaaaa gttactaaaa gtatataaaa
      541 ataaagaatg tttggaactt agctctgtag aatataagct tctaagttat ctaattgaaa
      601 ataaaggtca aatattatta aaggaacaaa ttttacacca tatttgggac agcgaagaaa
      661 attatgttga tgataatatt gtatctgtaa atataagaag acttagagta aaagtagagg
      721 atgacccatc taatccaaaa tatataaaaa cagcttatgg aatgggttat ttatggaatg
      781 aggtagaata atatgtttat attagccttt ataatatgta cagttgttat atttatagta
      841 tctactaaaa taaatcgaaa aagatatgat gaattaaatt tgatgttaaa tcaaatattg
      901 gaaggtaaag aagttactta tccagataca aaggatacaa gagcgtcaaa aatttctcac
      961 caagtaaaaa aaattaagga tatgatagaa atagaggtag aacagtcaaa gtcagaaaaa
     1021 gaagcgatta aaggtttaat atcaaatatg tcacatcaat taaaaactcc actttcaaat
     1081 ataaccattt attgtgaact tctggagaat ataaatattt ccacattaca gaagaaggag
     1141 tttttacaaa agatgaaaaa tgaaactttt aagattgact ggctgttaca atccttattt
     1201 aaaatgacta aattagagga tggagttata gaatttgaag tagaagaact actaattaaa
     1261 gatactctta ttcaaagtat cagtactatc tttaataaag cagaagctaa aaatataagg
     1321 ataaatctcg aaccattttc agacataaaa ttagttcata ataaaaagtg gaccatagaa
     1381 gcaattgtaa atgttttaga aaatgcaata aaatactcac catcagatag cacgattact
     1441 atttctgtaa taaaaatgga actatatacg aagattacta ttaaagatga gggaataggt
     1501 attgatagta gagaactaaa tgatattttt aaaagatttt acagaagtaa aaatgttgaa
     1561 aaccaaaatg gcacagggat tggtctatat cttacaagat taattttaga aaaagaaaat
     1621 ggaaacatta tagtagaatc taaactagga agtggatgtt gttttagtat cttcttacag
     1681 aattgtaaga gtttaaacta attttgtagg atgagtgtaa ttatcgtcac ttacaatatt
     1741 ttatagaaca tatatctgta ttgaaaggag aaattaaatg agtatattag tgacgaataa
     1801 tttagtaaaa cactatggaa aaggtgagac taaagtaaaa gccttaaatg gagtaagtat
     1861 agaaattgaa cgagatacat ttactgctat agttggaact tctggttctg gaaaaagcac
     1921 attgttaaat attataggtg gattagataa tccaacctca ggtgatgtaa taattaaagg
     1981 aaagaacata tctaaaatag gaaaaaaaga tttgacagtg tttagaagaa gaaatatagg
     2041 atttatattt caaaactaca gtttaatgcc cgtattaaac acttatgata atatagctct
     2101 tcctgttaca ttagataaag gaaaccatat agaccatgaa tatattgaaa tgctgatgaa
     2161 gacattggga atttgggata agcgtttaaa atttccaagc gaactatctg gtggtcaaca
     2221 acagagggta gcaattgcta gagctttggc aaataaacct gcgcttatat tagcagatga
     2281 accaacagga aatctcgata gtaaaactac aatggaagtg gtatgtttat taaaagaaag
     2341 tagtgcaaaa ttccatcaaa cgatacttat ggtaacacac aatgaaaata tagcacaagt
     2401 atgcgactca attatccata ttgaagatgg agttgtggtt aatactggtg gtgaattact
     2461 atgatgtctc tagtaaaatt ttcaattcaa tatataaagc attataaaaa acaatctata
     2521 tctatagttt taagcatagt actatcagta gcattattga cgggaattgg ttctttagtt
     2581 catagtgcag acaaaagtag aattgaaaag ataagggaag atagtggaga ttatcattat
     2641 ttctataaag tagataagga acaacttaat aaaattaaag aaaataaaaa atcaaaggaa
     2701 tacactgtag atagacttgg cgttacaaaa cctaaaaatc agatagatga accttttatt
     2761 atagaatttt taaatgctga ttcttcatat ttagacatga caggtagaaa attattaaaa
     2821 ggaactcttc ctagtaaaga tggagagata gtacttgaca catattctct aaacaattta
     2881 aatgtaaaca aagaaattgg aacagaaatc aatttagatg gtaaaatcta taagttatgt
     2941 ggtattttat caagtctttc aaacccagaa actgctctgg agggatttgt aaacgattat
     3001 tttcagccta aagaaaagga ttcttatata gtttatgtaa aatatgatga aaataaaaat
     3061 attaaaaagc aaaattataa atttatgaaa aaattcaaca tatcaaaaga aaaaacttat
     3121 ataaactggt ctctttcagt tgaatttgga gtaaaaccac caaaaaatat agaaaaatat
     3181 aatatatttg agttcttaaa aaatttagaa ctaaatacaa atgcaattat attgcttgtt
     3241 ggagtgtttg gtgcagcagc agtatatagt attttccatg tttcaatact tcagagaata
     3301 tcacaatatg gggttttaga agtattaggc gctaataata aacaactttt atttctacta
     3361 tttttagagt tatttttact ttttattgtt ggatttccta taggttgctt cttaggtatt
     3421 ggagtagcgt ctacaatata tgaacaattt ccacatatat ttttgagtag tgatattgtt
     3481 ccaggagcat tttttataag tgaaaaatct atttatttag gctttttatt tatcttattg
     3541 ttgttaattt taattgctgt acgtgttgta catcagttga ataaatactc tagtatagaa
     3601 tctatgaaaa actttaagat tactaataag aaaaagagaa tattagaaag tgtaaaacac
     3661 tctaacatga caaaagtatt atctcataaa tacatgactc aaaaaaaggg aatgtttatt
     3721 ggaattttgt tttcattatc acttggtgga attatatttc tatgttctag ttactctata
     3781 cagcttacta aaaataataa tgaacttaca atgaaagctg atgatggttt aaattctgac
     3841 taccaaataa ctatgcaaac aacagatttt gatgtaggta taccaaaaga taaaatttca
     3901 agattaaaca atgttgaggg aatatcaaaa gtctatccag taagatattt atttggagct
     3961 atgcaaatta aagaagaaca actattttgg aaaaatttct ttaaacctct tgaaaaagat
     4021 tacaggataa agactttttt tgatggtatt tgtacaaagc aaaatgaagg tggctatctt
     4081 ttaaaaacaa atatttatgg ttataacaat ggtatgttag aaggattaaa ttcttatatt
     4141 atagatgggg agattaatac ccatgatatg gttaaaaata ataaggttat agttagacta
     4201 cctatggatg gaactgggat gtatgatgca gttgatataa atccaggaga tacaataaaa
     4261 gttaaagtgc caaagacaat gaaaccaaca gatgaaattg ttaaatttaa agaagaaaat
     4321 aagaatgatt atacaacaaa agaatttgta gttgctgcta ctgtaaaacg tgtgatggca
     4381 aataacatat atttcattgg tgattatggt atggatatag ttatgacgaa tggtcaaatg
     4441 aaaaataact ttgatatcga aaattataat tcagtaagta taaaaaagac agaagatagt
     4501 aacagtgagg aattgtctga gaacataaaa agtgttgtaa gtgatgtaaa aagatgtatc
     4561 gtaacagatt ataccattgc tattgaaaaa aataatacct acttaaatca aaaactctta
     4621 tttatatatg gtattgtatt tgtacttctt gcaataagtt tgttccatat aatcaataca
     4681 gtaagctact taattttttc aagaagacat gagtttggaa ttttaagagc tatgggtatt
     4741 acggataata agtttttact tatgatgata agagaaggtt ttttatatgg attctatgca
     4801 agtattataa tggtaattgg aagtgttatt ggacaattta tgatttattt tatggtaaaa
     4861 cgtgtatatt tatatataaa tcca
//
LOCUS       DAGSWI010000204         4849 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.209, whole genome shotgun sequence.
ACCESSION   DAGSWI010000204 DAGSWI010000000
VERSION     DAGSWI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.209"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     repeat_region   45..1920
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=112..140
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(2579..4672)
                     /locus_tag="MJF80_003665"
     CDS             complement(2579..4672)
                     /locus_tag="MJF80_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBZ0282271.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         1773 a          739 c          477 g         1860 t
ORIGIN      
        1 atgctaatat aactagataa tcataagata acacattaaa agaaaattat attctgtata
       61 gttagtataa aacgtaagta atataaattt atgtaaagca acaaaagatg gatttacatt
      121 ccatatagtt aatataaaac gcttatgacc ttccctatat ccctggttct tatgatattt
      181 acattccata tagttaatat aaaacaaaga tttgattttt gtttctgttt atttatttat
      241 aatttacatt ccatataatt aatataaaac ttaataaaga catatttgtt attttcaaat
      301 cattttgatt tacattccat atagttaata taaaacctct tataagtatg actcctatac
      361 aaaaatttac gatatttaca ttccatatag ttaatataaa actaaagata gaataaagaa
      421 agtatgcttt tcaaagaaat ttacattcca tatagttaat ataaaacaca cagttgatga
      481 aacagaaaaa gaggttcttt tggaatttac attccatata gttaatataa aacaatgata
      541 taaaagacgt ttttggcgaa aaagttatag atttacattc catatagtta atataaaacg
      601 aaatctttat tcagcgatta caagaggaga tattgcattt acattccata tagttaatat
      661 aaaactaata tactttgtta ctattccatc tttaaatcca caatttacat tccatatagt
      721 taatataaaa ccaaaattaa aattttttag tgacctattc tttaaaaaat ttacattcca
      781 tatagttaat ataaaaccag aataataaaa gaagcgaaaa tatattagac tcgtatttac
      841 attccatata gttaatataa aacaagttgt agaggaacta ggttatccga tttttttgca
      901 atttacattc catatagtta atataaaaca cgacagcaga aaaccaaatt gatttatacg
      961 tgtgggaatt tacattccat atagttaata taaaaccgga ttttaaacct ccttttgatg
     1021 ttcctgttag taaatttaca ttccatatag ttaatataaa acttgcctgt tcgagaagta
     1081 gttaatactt tcgtgattat ttacattcca tatagttaat ataaaaccac ataatacttc
     1141 tcaaattcct aataattgtt atttatttac attccatata gttaatataa aaccttgacg
     1201 aaactaaact agtcataccc gttcaaaaca tttacattcc atatagttaa tataaaactt
     1261 ctagtcctgt atctataaaa acaatctcgt ctaaatttac attccatata gttaatataa
     1321 aacaaaagca aaaagccact ctcgtgcaaa gagtgactta catttacatt ccatatagtt
     1381 aatataaaac aatatgagct tatgaaagag cttgaaatga atatttaatt tacattccat
     1441 atagttaata taaaacaaaa atcaatacct ataataaaaa agttttgagt ttatttacat
     1501 tccatatagt taatataaaa cgcaaaatca aaaatttgac ttaataacac cattaattaa
     1561 tttacattcc atatagttaa tataaaacgg tgatactttt gggtaagctt ctttcaccta
     1621 gttggattta cattccatat agttaatata aaacctttaa ctgtatatac tttgttgtca
     1681 taggcagtac catttacatt ccatatagtt aatataaaac gaagtgttgg agaatataaa
     1741 aatttatatt taaaagttca tttacattcc atatagttaa tataaaacct gagctgatag
     1801 gagctgtgtt tggaggtgta aaaaaattta cattccatat agttaatata aaactggtgg
     1861 agctactgat attagactta ttggtggtaa catttacatt ccatatagtt aatataaaac
     1921 cccaaaataa acttagtatt ttcaacactt acacgtatac aactctctta aatttgcagt
     1981 gaacgagcaa tagtgcaatt gataacgctt atcatgcact ctcaacgcct tatatttcaa
     2041 ctgttacgcc aaattttttc acaaatatcg ctcactgcaa aattcctata attttattat
     2101 atcataaata tattactttt gaatattgat accaatttat accataataa aagcaaaaaa
     2161 acgtaatcta tctacattag atttaaattc tataacttaa agttttttac ataatattta
     2221 tgcttgttta atcaatctaa tatatcattc ttgtatattg cttttaatag tcttttttct
     2281 taaataaata tctatctgaa caagcatagt ttaattaaaa acttttgtat tcccttaaaa
     2341 ctgtatctac ctcttattat tttatttatg atatattatt tttaatctaa aaatagatat
     2401 ctatttttat tatggttatc cctttttaaa atactttaat catatactat tcccaattta
     2461 acatccattg aaatgctatt ttcttatatt gcaatttata attgatatat cttcaatata
     2521 ataatttaat gtatttttga ttttaaagct tttcagtgat attattgata aattagaatt
     2581 aattatctag ctttaaaagg tcaaagattt ctgttgctgt ctgcggtttt ccaaataaat
     2641 atccttgtat gtaatctggc tttatttgtt taactaaatc atactcttct tgtgtttcta
     2701 caccttctaa acatactttt attcctacgt catgacaaat tgcaacaata aaatgtataa
     2761 atgtagcatc aaacttactc tttaatatat ctttaacaaa tactctatca attttcacta
     2821 tatctattgg agaaaatttt aaaacttcta gagacgagta tcccgtacca aaatcatcca
     2881 tagcaatata aattcctaaa tcttgtaaag ccttaaattt aaactgtaaa atactcatat
     2941 tttgtactgt atgactttct gtcaattcta aaacaagatt ttgataagga aaattttcat
     3001 catctataat ttttactata tcttcaataa atgtgtcttc taatatttga acagctgaaa
     3061 cgtttacact cactttaaaa aaaggatagt aatcaatcca tttcctaaaa gtacgtaatg
     3121 ccattcgaag cacccaagcc cctacaatat ttatcatatc attttcttct aatattggaa
     3181 taaattccaa aggagaaatt gctttgcatt tgtcattagt ccaccttaaa agaacttcta
     3241 cacctattat tttatgactt tctgtatcta cttgtggctg gaatcgaaga ctaaaccccc
     3301 taaaatcatc attaattgat gcctttaaat cccgcatcat ttctaatgaa tacattttat
     3361 tttttaatat ttcttgggag aaaaaaacaa gcctgttttt accatgctct ttcgcatatt
     3421 gtaaagagta actagcacac ttataaagtt cttttactgt atctccatgt tttggatata
     3481 ttacacaacc agcagaaatt gttatattta atccatattt tctccatagt tgaaggtgta
     3541 taatcatatt ctgtatttga ttatatagag ttagaatttc attttcttca acattatcaa
     3601 caagaatacc catttcgtcc ccatcaagtt tataaagttc agcattatct ggtaaaatag
     3661 attgtataga ctgtgctagt gtttttatta tattatctcc aaaatcccta tcatacatct
     3721 cattaacatt tttaaaatcg tcaatattta ataatactat gcataatttt tcaatcatag
     3781 gatttgaaat tttatcttca aatacactca taaattcatg ataattcagt aactgtgtca
     3841 atgaatcaat cttattttgt ttacctaatt gcgtcattat tcctgcaaaa atacttggtt
     3901 ctccaaattc atcacgcatt agttgacctc tacatcttaa ccatatatat tctccactac
     3961 gatttttagc cctaaattcc actgtatgat aatccatctg gtttttacct atttcagtgt
     4021 ttgacttata aaaacgattc cagtcttctg gatgtacaat ttttttccag taaataagcg
     4081 gattttcaac aatttctcct ggtaagtcaa ataattcaac ttgtgatggt gaatatcgaa
     4141 atacgcctgt tttcatattg caaatgtata tatattcatc tgtacctcta atcaaagcat
     4201 cgtaaagcaa tgtagaatta taatcaaaat tgtctcttga aaaattaaaa actccttttt
     4261 cttctttatt atttgatatt tcatgaaatt cattaatctt aattggttta tttaagcttg
     4321 ttgagtttgc aatatctatt gattgatgca tatgaacaac tgtgccatct tgccatgtaa
     4381 ttaaactatc ataattttca aaaacacgat ttaacttatt acatttttca taccacttta
     4441 ttaatactcc ttttttatca ttctttaata attctaaaac tttacaaaaa ctacaagtag
     4501 aatttttttc aggatataat acttgccaac aaacttttcc ttctggatct aaaatattat
     4561 attcttcttt catttttttg ttcataaaaa taatttcatt agtatgaata tttgtaacat
     4621 atatattaat ctgtagttga tttaatataa cttcaaaatt atgtttgttc atatacttat
     4681 ctcctccata gttactgctc tttataacaa cattgttaaa attattcttt ttagaagatt
     4741 tacttataac ttatacatat acactaaata ccttaattta attagaaagt ttattacttc
     4801 atattttaaa ttataattga taaaaaaaaa tacagcaata tagtattac
//
LOCUS       DAGSWI010000205         4844 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.210, whole genome shotgun sequence.
ACCESSION   DAGSWI010000205 DAGSWI010000000
VERSION     DAGSWI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.210"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(83..619)
                     /locus_tag="MJF80_003666"
     CDS             complement(83..619)
                     /locus_tag="MJF80_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBZ0282272.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(732..1406)
                     /locus_tag="MJF80_003667"
     CDS             complement(732..1406)
                     /locus_tag="MJF80_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBZ0282273.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            1609..3483
                     /gene="asnB"
                     /locus_tag="MJF80_003668"
     CDS             1609..3483
                     /gene="asnB"
                     /locus_tag="MJF80_003668"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBZ0282274.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(3575..>4844)
                     /locus_tag="MJF80_003669"
     CDS             complement(3575..>4844)
                     /locus_tag="MJF80_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBZ0282275.1"
                     /translation="KIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYDS
                     SKFNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLC
                     WIDYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDL
                     YPECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTN
                     LTSKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLH
                     VVNDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVNRKCKPIPVKVSKKPVRGQK
                     IVAIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLIS
                     AGYDDGQNLNLAVESSLVKIFANNFIEIVN"
BASE COUNT         1877 a          579 c          699 g         1689 t
ORIGIN      
        1 aatttttagt tcttttgtaa actaaaatat ataaaactta tttatactta gttcaaaata
       61 ttgttattta ttttttctac aactataata aaatataaaa ttgatatcca atcaaaaaaa
      121 taactatgaa atttaatagc ttagatatga atttttctgt ttttgagtta gtttttatag
      181 gtagtaaaga aatgtttatg gtaatgggat aaaacaattc aataccttct ttagtaataa
      241 ggtcaagaca caagtgagat agatatccta ttataaaccc actggacaaa catgagaaaa
      301 ctatattatt gttagtgaag attgtcagag atttaaaaaa actataaata agtaataagg
      361 caattagact gtgagtaaac cctctatgtc taaacctact tccaaataat ttgtatatta
      421 acataaattt tttacttatg taagaacctc tcatatctat atctggaaat aaagaaccta
      481 aatatgcaaa ataaatataa attataatta atactatttt atatgcaata tcgtatttaa
      541 ctaggtatag attgtttata aaggggagta tcaaaagtgc gaatatataa cctccccttt
      601 catgtgtttc ttttatcata taaaaatcct cctgaatcgt cactataaac attatattgg
      661 taaatttaga ctatctttta ttatattata tagcaaacat aagttttttt caaaaggatt
      721 ttaatattta attaatcttg agattttaaa tttttgtaat tttctaaagc accattccaa
      781 tttactacat taaaccagtt gtcaatgtat tcatttcttc tattttgata ttttaaatag
      841 taagcatgct cccaaacatc aagtcctatt ataggagtta ggtttttact tacagggcta
      901 tcctgatttg gagtagtcat aatagataat ttgccatctt tagtagctac aagccaagcc
      961 caaccagaac caaagacatc taaagcagat ttttggaact cttgcttaaa tttttcaaaa
     1021 gaaccaaagt ctctatcaat agcttctttt aaagattcag aaggtatggt tttttctggc
     1081 gtcattatat caaaaaagaa tttatgatta taagctccac ctgcattatt tcttacagtt
     1141 gtagcaatat ctttaggtaa agaatctaaa ttttgcaata attcacataa agaataatta
     1201 taaagctcag gatatttttc aagagcagca tttaatttat caacataagc ttgataatgc
     1261 ttatcatgat gcagtttcat tgtttcttta tctatataag gttcaagtgc atcatatgca
     1321 taaggtaatg gtttaacctt aaatttgtta ttttcaggtg taaaagcaaa tgaagttatg
     1381 cactgtgaga ttataaataa agacataata actggtatta atattttttt cttcataaaa
     1441 atcacctcct aatatagtat ttctagaaat aaaaaaaata tgaaatatta acagataatt
     1501 tataagaatt taatagcaat ttcattttaa attgaataag ttttaaagcc aaagacaaaa
     1561 ctatgaaata tagaataaga ctaaaggaca gaaaggaatt ttgattatat ggacggcaat
     1621 atttcaattt attataaagg tgatatatct aaaaaagaag atattaaatt taaaataaaa
     1681 gaatctgtaa ataatacaag actttctagt agaatagagt ataaggatga ggaaaaaact
     1741 tttgattttg aaagatttaa tgtaattgat gctatttatg gaaataaacc atttgaaaaa
     1801 gatggaaaag ttattgtttt taatggaaat atatataact atcatgaaat aaaagaagaa
     1861 ttaattaaaa aaggacacaa ttttactaca aatgatgata ttgaagtctt attggcatct
     1921 tatatagaat ttggtaagaa ttgtgtacat aagattaaag gaatgttcaa ctttataatc
     1981 tatgatagag aaaatgaaag tatatttgga gcaagagatt tatttgggat aaaaccactt
     2041 tattatataa ataaggaaaa tgcaataatt ttttcctcag agtataagtt tttacttgaa
     2101 tatatgaaaa atttaaatat aaatgaaaga agcttacaaa gttatttttc atttcaatat
     2161 gtacttcctg aagatactat gatacaagga ataaggctga taccagcagg tcactatttt
     2221 agggtagaaa atggtatact atctttaaag agatataata aacttgagtt tagaagtagt
     2281 acaaaatttt tttatactaa aaatcactta ggcaatagag atgtgaacga ggatgatata
     2341 agaaatatag tagtagattc aatagtaact catatggaag aggagaaaga aattggaaca
     2401 tttttgtctg gtggaataga ttcatctatt ataactactg tggcttcaca aattaatcca
     2461 aatataaaat ctttttctgc aggctttagt gtcaaaggat atagtgaatt agaagtagca
     2521 aagaaaacag ctgataagtt aggtatagaa aatatccaga ttaatataac acaagatgaa
     2581 tatataaaat cattaccgaa tgtaatttat tctttggatg accctatagc agacccttca
     2641 gaagtaggtc tttatttctt aattaaagag gcaggtaaac atgttaaagt agcattgtct
     2701 ggagaaggag cagatgaatt atttggagga tataatatat ataaagagta tagtactatg
     2761 aaatcagttg tcaattcacc tacttatatt aaaagtatat taggaagagt ttcagaactt
     2821 atgccaaaca taaaaggaag aaattatctt tatagagcta caacaccatt agaaaaaaga
     2881 tatataggaa atgcaaaagt ttttgaaaac agcgaagtaa aaagattctt ttttaagtat
     2941 aaggaaaaga atatatatga atacctgttg tctaatttgt atagagatgc acaaaaaaat
     3001 aattacgatt atataagtaa aatgcaacat atagatgtga atacatggct tcaaggcgat
     3061 atacttcaaa aaattagcaa actttctaca gctgagcagg ttgaacttag agtaccattt
     3121 ttatataaag atgtatttga tgtagcaaaa aatttaagaa tggagcaaaa aataaataag
     3181 aataatacaa aagttttact tagagaggcg tttagagaaa tagtcccaga acatgttgtt
     3241 caaaggaaaa aattaggatt tccaactcca ataagagttt ggttaaaaga ttctctaggg
     3301 gatattgtaa aggaaactat ttataattca aatgtagatg aatttataga taaaaaatat
     3361 gcaataaaac ttttagatac gcatcttaaa ggtcatagag ataattcgag aaaaatatgg
     3421 actatattta cattttgtct atggcatcaa ttatttattg agcataaaaa tgtcgagtat
     3481 taatactgtt ttcaatatta atcagagggc aaaagcatgc aatgttttca ccctcctgat
     3541 tattatggaa taggataagt agaattttga ttgattagtt tactatctca ataaaattat
     3601 tggcaaatat tttgacaaga ctactttcaa cagctaaatt taagttttgt ccatcatcat
     3661 atccagcgga aatcaatcct agcaagtttc caaacatatc caaaagagct cctcctgaac
     3721 taccactaga gattggagct gtaaattgta tcatttgaac cgtttcaaaa tcacgaaaag
     3781 ctgagataat tccatctgaa actgtattaa aaagacctaa agggcttcca attgcaacta
     3841 ttttttgacc tctcacaggt tttttactga ctttaacagg tattggcttg cattttctat
     3901 taacctttat cacagctaaa tcataatctg aatggtattt tataatttgg taagatgtat
     3961 aaactttatc atcattttca aatctaacta aaaatgaata cccatcattg actacatgaa
     4021 gattcgtaag tatataacct tcattattta ttactactcc agaaccgctc ttaaacggtt
     4081 tattttcgtt attgcacaca acaatcataa caacagaagc tgataacttt gcaaggtctt
     4141 cataagaaag ttctacttcc tcatcttgta caattttttt agatgttaga tttgtgttat
     4201 taagtgtatt tttagagtta ggtttattcg tagtaggaga agtcttattg gtattttcac
     4261 tgtaatttat atttgtaata tgcctttcaa aaggtatttc taatatattt ggagtcttcc
     4321 tagaaagttc aaatagagta gaaaattttg aacattcagg atataaatca agaatatctt
     4381 tatttggatt tatatataaa atatcacacc caagttgtga taaaaaatat aagaaaaaaa
     4441 gttcttgcct tttaatattt ccaaagtata ctatctttgg tgaaatagta gtcttcacct
     4501 tgttataaaa taatttaggt agaaaatagt ctatccaaca caaaagttta actgcaaagt
     4561 ttttgatgat agaggatgta caatttggca tacttgtgga aaataaatct aaagtatttt
     4621 taaatgcaat ctttttagtc caatttaaag tgtcattttc tattttacat ggaaagtcga
     4681 tattatataa atcccaactg ttaaacttag aactatcata aatattaaaa attttaagca
     4741 tcttatctgt ttgttctttg tcaaaattta tttttagatt gtttaatcta atatagggaa
     4801 ttgtaggatt attaaaaaaa taagtatcta tatttttaat tttt
//
LOCUS       DAGSWI010000206         4830 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.211, whole genome shotgun sequence.
ACCESSION   DAGSWI010000206 DAGSWI010000000
VERSION     DAGSWI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.211"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            64..1692
                     /gene="aspD"
                     /locus_tag="MJF80_003670"
     CDS             64..1692
                     /gene="aspD"
                     /locus_tag="MJF80_003670"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBZ0282276.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEGLFKFISKNASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1813..2808
                     /locus_tag="MJF80_003671"
     CDS             1813..2808
                     /locus_tag="MJF80_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBZ0282277.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(2997..3380)
                     /locus_tag="MJF80_003672"
                     /pseudo
     CDS             complement(2997..3380)
                     /locus_tag="MJF80_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3606..3703
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3845..4340
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3845..3873
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(4626..4784)
                     /locus_tag="MJF80_003673"
     CDS             complement(4626..4784)
                     /locus_tag="MJF80_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282278.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
BASE COUNT         1714 a          664 c          736 g         1716 t
ORIGIN      
        1 tttttgcaac ttgtttattt tattataaaa atgtgaaact tattacataa ggaggaattt
       61 aacatgaaaa atcatactta taaagaaatc aaaaacatat atggaaaaat aagccctttt
      121 gaatttaagg acaaattaat agatattgca aaatacactg caaaagaaaa taacagagaa
      181 ttacttgatg caggtcgtgg aaatccaaac tggacttgtt caactgctag agaagccttt
      241 tttacttttg gtcatttcgc tattactgaa actcgttcta attgggattt aggtcatttg
      301 gctggaatgc ctcaaaaaaa gggtattaaa gaaagatttt ttaaatttat aaatgaaaat
      361 atagatatgc ctggagcata tttagctaga gatattataa actttggtat aaatgaactt
      421 ggatttgatg gagatgaatt tgttcatgaa ctagctgatg gaattattgg tgataactat
      481 ccacttccag atagaatgtt acctcatatg gaaaaaatag tacacgatta cctagttcaa
      541 gaaatgaaat atgatatatc aggtaaatat ggtgatgtag aaatttttgc tgttgaaggt
      601 gctacagctg ctatgtgcta tatatttgat tcattaatgg caaatgaact tctaaaaaaa
      661 ggagatacaa tagccttaat gactccaata tttacacctt atcttgaaat acctaacctt
      721 cctcgttacg actttaaagt ggtaaatata aatgctaatg aagttgatga aaaaggtgct
      781 cacacatggc aatatacaaa aaaagaatta gagaaacttc gtgacaaatc tattaaagct
      841 ttattcgttg ttaatcctaa taatcctgct tctatagcta tggatgaaac atcttgtaat
      901 aatctaatag atgtaattga aaattataat aaagatttaa tgataatatc agatgatgta
      961 tatggaactt ttgtagaaga tttttcatcc ttaatgtcaa aactacctta caacactgtt
     1021 ggagtatatt cttattcaaa atattttgga gttacaggat ggagacttgg tacatttgcc
     1081 cttcataaga aaaatgtttt tgataagaaa ataaatgatt taactggaga attaaaaaaa
     1141 tcagtagata agcgttacag tgatatgagt cttaaccctt ctagcctttc atttatggaa
     1201 agagttgttg ctgatagcag acttgttgct ctcaaccata ctgctggtct ttctactcca
     1261 cagcaagtac aaatggcgtt tttctctgct tttgcattga ttgacaaagt agatgcctac
     1321 aaaaaactca atatgagtat ctgccataga cgtcaaaaac tattatttga agctttagag
     1381 cttccaatta atgaaaacaa aaacaatgct gcatattaca ctcaatttga tattgaagaa
     1441 tgggctaaat taaactacgg agaaggctta tttaaattta taagtaaaaa tgcatctcca
     1501 gtagatgttc tttataaatt agctaatgac tattcagtag ttttacttag tggaaatgga
     1561 ttctatggtc ctgaatggtc aattagaata tctctagcaa atctatatga tgaagcttat
     1621 actaagatag gaaaagccat aagagagata ttaaatggtt atatcgttga atggcaaaaa
     1681 attaagaaat aattatatta cactgaaata tctaaattat atgaataaaa gagtttatgt
     1741 agaccttaac tacttttttt gatataatcc tatcggaatt ataaatttta tttatgaaag
     1801 gcagagaata gtatgagtat taagaaaaaa gttgccatag tttttactgg tggaactata
     1861 tctatgacag tagatgataa agtcggagca gctataccaa ctttatcagg tgaacaaata
     1921 ctatcaatgg ttaccaacat tgataaagtt gcagatgttg aagtttttaa ttttgatgaa
     1981 attccaggac ctcacataac accttataga atgatggaat tgaaaaacta tgtaaatgat
     2041 ttattagcaa gaaaagatat tacaggtgtt gtaataacac atggtacaga tagtttagag
     2101 gaaacagctt attttctaga ccttacaatt gacaacatca aacctgtaat agtcacaggt
     2161 gctatgagaa gtagttctga acttggatat gatggttcaa gtaatttatc tgcatcagtt
     2221 tgtactgcag tatccaaaga tgctatggga aaaggagtcc ttgtagtttt aaacaatgaa
     2281 gtattattag cttctgaagc tactaaaaca aatactcttt ctctaaatac atttaaatct
     2341 ctaactagtg gcccattggg aataattgac tgcaacgaac ttgtccttac aagagatatt
     2401 gtaaatagaa caattataga tactgataag gtagaatcta aagttgcctt atttaaagca
     2461 tatgtaggag aaaatgctga ctttataaga tttgctgttg atagtggtta taaaggtata
     2521 gttattgagg caatgggcag aggtaatatt cctcctcaaa tgctttctgg agttgaatat
     2581 gctagagaaa aaggacttcc tgtagttata gtttctagat gtcattcagg tagagtattc
     2641 gatagttatg gttactttgg ttctggtaga gatttaaaaa atcttggttg tatatttggt
     2701 ggggacttac caggtcaaaa agctagaata aaacttatct tagcacttgg aaaaactgat
     2761 aaccttgatg aaatcaaaga tttctttgaa aaaggaatct attgttaatt ctaataactt
     2821 ctatttaatt atttattgat atttcaacat ttttaaatca aaatctaata cttatgtcag
     2881 ttaaatgtca gcaaaatatt ttatccacaa tttttacaca aattttccac aacttatccc
     2941 caatattatc cacaagaaaa aaggtagcac ttatattagt agctaccttt tatttattaa
     3001 tcttcatctt tccagctttt tatccaattt cctatttcta ttattttttc ttcagttatt
     3061 tcttctccat catgtaattt tgaaattagt tctgataatg gattgttatg tagacctcca
     3121 aaaattcttt ttccctctat tttgagatat tctttctctt ttattaatac tctatagtgt
     3181 acatgtttcc caattctctg cgatatgaaa aaaccttttt tagttaatct agctaaaagt
     3241 gtaattgtag ttgtatgctt ccaagcatat gttttttcta tttaatctgc aacatctttt
     3301 gatattacag ttttataacc agtattccat atgtatttca taatttttaa ttctgcatct
     3361 ggtaattttt tattcaacat aatatattcc ttctttcaat ttatgtcact tcctcaaact
     3421 tatctagctt aaattaataa taggagcttt ttatcacaaa aactacaggt aaatttttct
     3481 atatattcta cgtatttagt ctgtttttaa gtgtgaaatt acgttcatac ctataaattt
     3541 attcaatttt caaacaatct tgttgaataa taactaattc tattgtatta tttagttata
     3601 gaataaaact aaatcggcaa aactagagaa atctagtgac gcaaagctat agggactaag
     3661 acttatataa atatcttatg agttatgtta gccagttgcc aaagagatat tgttcttttt
     3721 gtttttatga aagtttttta gggggataat aatttatatg tttagagata aaatggataa
     3781 atgtacacat atattgactg cttatattag cagttcgtat gattattgta attttttgga
     3841 tacaatttac attccatata gttaatataa aacttgtgtt tattgtgata gtgccgaaca
     3901 ggtgttaata cgatttacat tccatatagt taatataaag ctctaattta atagctttat
     3961 ttttagcaaa atacccaaat ttacattcca tatagttaat ataaaaccgg ttccctccga
     4021 agttcataga gattcttatc agcatgattt acattccata tagttaatat aaaacaaatg
     4081 ctgaaagata attcggttgg ggatttaatg aaatttacat tccatatagt taatgtaaaa
     4141 ctaaatagag cagatgaggc ttttcaagaa gccgatagat ttacattcca tatagttaat
     4201 ataaaactct gatgttgtga gatttgaaaa aaacggtaaa ttgaatttac attccatata
     4261 gttaatataa aacgctgata aagttattaa aagtaaaatt gctgaaatac tatttacatt
     4321 ccatatagtt aatataaaac cccaaaataa attgagtatt tccaatgcct acacatatac
     4381 aaatctctca aatttgcagt gaaccatgag tagtgcaatt gataacactt atcacaagtc
     4441 ctcaatgcct tatatttcaa ctgttaagcc ttaatttctt caaaaaatcg aacactgcaa
     4501 aatttctatg actttattat atcataaaaa tattattttt gaatatctgt accaatttgt
     4561 gatataataa aagcaagaag aactacaatc tattttgctg tagagtggag ttcataattt
     4621 agaaattact tagttttatg gaatctgatt tttaaatcaa attcccaacc actcttagtt
     4681 gccgctttga gtggtttttt acgctttcta aatagtttgc tagctatata aactattaag
     4741 ctagcagata gacttgctag tatgccttgt aaaaaattat ccatgtatat tcacctccct
     4801 tcttagcgtc gggaggataa ttttttgata
//
LOCUS       DAGSWI010000207         4689 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.212, whole genome shotgun sequence.
ACCESSION   DAGSWI010000207 DAGSWI010000000
VERSION     DAGSWI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.212"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            422..4564
                     /locus_tag="MJF80_003674"
     CDS             422..4564
                     /locus_tag="MJF80_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBZ0282279.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTNPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDAVVESIPN
                     PNPTNNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVVSADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDLPASIINPEYSLDGGVTFQPWNGSLNIGTLEVGETK
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NISTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVIFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVILLRGIIKSTCLDRLTNI
                     AEVTSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKNCCCNCCKNDF
                     WC"
BASE COUNT         1795 a          696 c          802 g         1396 t
ORIGIN      
        1 tatcttaaat aattaagttt aaattttacg atattttgtt tgatgattta taatataaaa
       61 gcatactctt aatcttcaaa atttaaaaag agtaaaaaat aacaaaaaaa tcactgacat
      121 ttcactgaca aattgtaatt ttgttattat aaatacattg aaaatacttg attattgaaa
      181 aatggcataa aataatacat tttatgccat ttactattat ttgatagttt ttaacttatg
      241 acataagata ttttttaaca tatacaataa aatataaaaa aattgatatt gcaaataaaa
      301 aaaatacata ttagatgtta tagaaaaaaa gcgcacttat aggcttaaaa tgtcacataa
      361 aagagtttta aagaataata tattatgaac acaaattttg taatcacaaa ggagtgataa
      421 tatggcaaca gtatcaggac aaatagtttt tgatagaaat agaagtgcta ccattgattc
      481 tggagactca ggaatagcca atataccagt tgtattacaa gatatagcaa ctggagttag
      541 acttgtggtt ctcacagatg catcaggaaa ttatgttttt actaatgttc caaatggtaa
      601 ctatagaatt gtagaagctt ttggaactac aggaggagta ccaacaccag ggaattttgc
      661 aacagcgata gtaggtccag ttccaattgc agaaatgcca cctattagtt ctgtgacaaa
      721 tcctcctatt ggttcaacaa acttagatgg tacaacacct aacacactat taataacagt
      781 agcaggttca gatataacaa atcaaaatat attaaatggg cctgtgattt atacacctat
      841 agagaatata ttggattctt gcgtatctgt atctaatata aaccttatta cagatgcaga
      901 taacggaaca ttcggttttt ttccaccagg tacaccaagt aatacaggac ctgaaataga
      961 accttaccca aatgtgacgc cagattttac gtatgttgta ccagacccaa ctaagtttac
     1021 acctttagat ggagaatata cagtacaaaa tattatgaca aatgcaatga gtaatgttat
     1081 aggagcttgg tggcgtattg ctgaccatac tacaggaaat gaaacaggaa gaatgatggt
     1141 ggtaaatggt tttaatcctg gagctgtatt ttttaaatca acagtacaag ttacacctaa
     1201 cactaattat ttatttagca cttggatttt aaaccttttt aaggtaacag gatatccacc
     1261 accacagcta ggtgtaagaa tatttgacca aaataataat atactatatc aagcaacatt
     1321 aggagctcaa attccagtta atacaaacgc accagaatgg aaagaagtag gtactgtgat
     1381 taattctcaa aataataatc aacttacagt agaatttttt agtgaaggag aaagcgccat
     1441 tggaaatgac tatgctatag atgatgtttc tttaagagaa atagctgttc caacttttac
     1501 tccaataaaa acaagtaaca cagaaactgc taatgtaggt gatataatca catatacagt
     1561 tacattagat aatacttgtg atagcccttt aactaatgta ttttttaggg atttaattcc
     1621 aaatggttta gagtttatac ctggaactgt aacggttgat ggagtatctg tgccaggagt
     1681 agaccctaat acaggtttcc cacttccaga tattggagga ggaacagggg ttgaagtaac
     1741 ttttgatgct gtggttgaaa gtattcctaa cccaaatcct actaataata tagctaatat
     1801 agattactcc tatacacctg tagaaggcgg tataccaaat gatttttcag tagattctaa
     1861 tccagttcct gttgaagtag tatcagcaga tattgaagtt acaaaattat ctgaacctac
     1921 tatagtgaat ccaggagagg aattaatata cactataaaa gtagtaaata atggaccatt
     1981 cccatctgag aatgtggttt taacagatga tttaccagct tctattataa atccagagta
     2041 ttcattagat ggaggagtaa cttttcaacc atggaatgga agtttaaata tagggacatt
     2101 agaagtagga gagacaaaag ttattataat taggggtatt gtaaatccat caacagtagg
     2161 aattataaca aatacagctg tagtatcttc aactacagca gacccaaacc ttaataataa
     2221 tacatctaca atagagacag aagtgaattt attagcagat attttagtaa tgaaaacagc
     2281 agaacctaat tcagctgttc caggtacttt attgagatat actattcaag tagaaaactt
     2341 aggaccagca aatgctgaaa atgtaatatt aaatgatgat attccagctt ctattataaa
     2401 tccagaatat tcattggatg gaggagcaag ttttcaacca tggaatggaa gtcttaatat
     2461 aggaacttta aactctggaa tttcattaac agttttaatt caaggaacag ttagtttaaa
     2521 ttcttcagaa tatatagtaa atacagcaac agtttcttca actacacctg acccagattt
     2581 aagcaacaat atatctacca ttattacacc agttaaccca caagcaggta tttctattat
     2641 aaaagtagct gacgaggatg tagctgttcc aggagaagaa tttgtatata caattgaaat
     2701 ttttaatgaa ggaccatcta atgctacgaa tgtagtttta acagatgata taccagatgt
     2761 tattttaaat ccagaatatt cattggatgg aggagcaact ttccaaccat ggactggaag
     2821 tcttaatata ggtacagttg caccagggca attaataaga attatcataa agggattagt
     2881 aagttcaact gctacaggag atattactaa tacagcagaa gtaagtgcag atgtaccaga
     2941 accagttacg gattcatcaa cagtgacaac tcctatagtt ccatcagcag atatagaagt
     3001 aataaaaact agcaatatgg atacagcagt tccaggagaa actttcagtt atactataac
     3061 agttacaaat ttaggaccat ctgctgcaca aagtatcgtt ttgacagatg atataccaga
     3121 tgttatttta aatccagagt attcattgga tggaggagta actttccaac catggaatgg
     3181 aaatcttagt ataggcactt tggatgctgg agaaataaga agtattataa taagaggaac
     3241 agtgagccag acagcagtag gtacaattat aaatacagca acaataagtt caactacacc
     3301 tgacccaaat ccagataata atacttcaac agatgaaaca gatataagtc cattagcaga
     3361 tatttctgtg ataaaaggca acgaaccagt tgctatacca gggggaagat ttatttatgg
     3421 aattgaaatt gcaaatgcag gaccatcttt tgcagagaac gtaactttaa cagataatat
     3481 accagcttct attttaaatc cagagtactc aatagataat ggagtgactt tccaaccatg
     3541 gaatggaagc cttaatatag gtactttaga tgctggagag ataagaaata tcataataag
     3601 gggaacagta agtcaaacag caataggcac aattataaat acagcaacag taagttcaac
     3661 tacacctgac ccaaatctaa ataacaatat ttcaacatca gaagcagagg taagttcatc
     3721 agcagatatt tctgtagtaa aacgaagcaa tcagactgtt gttgtaccag gagatgtact
     3781 agactataca attgaggtga gaaatgcagg accttctact gctcagaacg taactttaac
     3841 agataatata ccagcttcta ttttaagtcc agaatattca atagataatg gagtgatttt
     3901 tcaaccatgg aatggaagtc ttagtatagg cactttagat gctggagaga taagaaatat
     3961 cataataagg ggaatagtaa gtcaaacagc aataggtaca attataaata cagcaacagt
     4021 aagttcaact acacctgacc caaatccaga taataatact tcaacatcag aaatagatat
     4081 aagttcatca gcagatattt ctgtaataaa attagcaaat aaaacagaag catgtgtagg
     4141 agagcaaata gattttgtaa tagtagtatc aaatgcagga ccagaaaatg ctcaaaatgt
     4201 tactttaata gacaatgttt cagataagct taagaaagca gtattttcac ttgatagagg
     4261 tgtaagtttc caaccatgga ctggaagtct taatataggt acactaccag ctggaacttt
     4321 aagagttatt ctattaagag gaattataaa atcaacatgt cttgatagat taacaaatat
     4381 tgcagaagtt acttcaacaa caccagattc aaaccttaac aataatatat caagagtgca
     4441 agtagaaata aaacaatgtt gttgtaatga ttgctgttgt aacaactgta gtaattgttg
     4501 ttgcaatgat tgtggttgta aaaattgttg ctgtaattgt tgtaaaaatg acttttggtg
     4561 ttgattttat aaaataattt tgtatacctt aaattaaact gaaataaaaa ataaattcac
     4621 atatgagtaa aatattttat aaatattaat aaattttcta tctttgatat ggaccacatt
     4681 cgtaatact
//
LOCUS       DAGSWI010000208         4688 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.213, whole genome shotgun sequence.
ACCESSION   DAGSWI010000208 DAGSWI010000000
VERSION     DAGSWI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.213"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            30..2936
                     /locus_tag="MJF80_003675"
     CDS             30..2936
                     /locus_tag="MJF80_003675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282280.1"
                     /translation="MDLSVSTIINDIHHRKYLESLNLPNPKEKAIIEINKNISFYLFE
                     KDIFNFTYEDLHKLIGEIYPRCDYISLNKIIAYIAESFFDTSMTTGNYIFSYKHRRFS
                     EYFTILKLEDKIRNDLNYLRENNIIINHDLFESMLIPYLQIKACNKKDLPLAFQIGLF
                     NVYLGNDKAWGIDKEFYIWSDWIIYAIAGLDNEIFESIMLDSSLPISKFFNEVPKKIK
                     ALLSSKSKKNLSYNDELRQNFKIYVVLISLMHKFGKLEFLSVLLQDYKEIRRLCKENK
                     FCFYSTSNKDNFLVWRSIIYINTVILNDNIDELISLVLQNSKDTNIDGLLKDRTSMNI
                     LHLNSLYYNMLIYHKNKCANMIENMNINQLSIFAITATNPECLNNIMKDKSLIEKIKQ
                     VLEDEISNENLSGVICLALKKFLGGSLVKKEEELVKKYLENIEFIGSSIFWKGHCDIS
                     AFILSVFGKGQIKLKIDQEIKRYVNVYKVFLELIDKSCSIERFICCVQKNIFNNTQDS
                     YYIRILLGKVLSLLNDEDFNIKGSLDYLNDSINDSRILIIYCTMKLYNPSRFKSLLSI
                     TELTKLTEPRVYEDVNFTSTSESLFMLSFILSNHDKLLGHELLLKGISNGIMRMNDRK
                     DTIGDYQLLDSLEILLINNWISTEKLIILLKRILKITNKMNLYNIENDTHARVMDIIQ
                     KYDFAAAEFYYNKIAHLEECYNLIHYRYALSMIQRGRNIKYIEACLDNIKLSFDRYHQ
                     KAEWGSFYYKIEVYLKISASDFYSSYEQKLSFEKACYKIDELEHAGWSRELKKEEYDI
                     YVELCKKYNKKIDIEKEKENEYCYSVKPLEKENQDILEIINSINTEEDLCEFIKRLDG
                     EYYLENLEINELFIKKCVDINGNIEVIIDMFTRYNYPSSLYYSRNSHNFWMTVLSALK
                     NAKSKNSMMQYLLENGGGHDGFSELIKIYGYLGNKDICIKLFERMLTCVEFLLC"
     gene            3280..4356
                     /locus_tag="MJF80_003676"
     CDS             3280..4356
                     /locus_tag="MJF80_003676"
                     /inference="COORDINATES: protein motif:HMM:NF025448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4263 domain-containing protein"
                     /protein_id="HBZ0282281.1"
                     /translation="MKINDFIFRFKYRNDEKSLCRVRTFLGRENKIINLITDLEDLNI
                     SKVSLINSIEFACSELIEKNMTCEDAIFIEHTESESLEEQKFDMVTFNPRGGPIWKNI
                     NINEVKKILNCIDDELINTTKKNRNLVETIDRIRYRINPETDFLYKESPEVIKRKLEI
                     KENRISKSKLEDLIKNRSKEQEIARLLKSDLSVFGEVYSFPKDEYICFSEYPIYDRYI
                     DYVVITGRSRMQVYLIEIKGAEFNLVNKDSKFNEKINEADSQISRTLGYIFRNYMAFK
                     DDIHEKRRLVEKGEKIFNSFRGPISKLEVDRQKDITIHSVIIGGVTNDDIKESRLRHD
                     YENKNDFRIKLESWDTFIRKLSNR"
BASE COUNT         1961 a          372 c          733 g         1622 t
ORIGIN      
        1 ttaatgggaa gagttttttt acaaacttaa tggatttaag tgtatcaaca ataataaatg
       61 atatacatca cagaaaatat ttggagtctt taaatcttcc taatccaaag gaaaaagcta
      121 ttatagaaat aaataaaaat atatcttttt acttatttga aaaagatata tttaatttta
      181 cttatgaaga tttgcataaa ttgataggtg aaatatatcc cagatgtgat tatatttcat
      241 taaataaaat aatagcatat atagcagaaa gtttttttga tacttctatg acaacaggaa
      301 actatatttt ttcatataag catagacgtt tttcagaata cttcacaata ctcaaattag
      361 aagataaaat tagaaatgat ttaaattatc ttagagaaaa taacataata ataaatcatg
      421 atttatttga atctatgctt attccgtatt tgcaaataaa agcttgtaat aaaaaagatt
      481 tacctttagc atttcagatt ggattattta atgtttactt aggtaatgat aaagcatggg
      541 gaatagataa agaattttac atttggtcag attggattat atatgctata gctggattag
      601 ataatgaaat atttgagagt ataatgctag acagctcatt accaataagt aaatttttta
      661 acgaagtacc taaaaaaata aaagcattac tttcttcaaa aagtaaaaaa aatctttctt
      721 acaatgatga attaagacag aattttaaaa tttatgttgt acttatttcg ttaatgcata
      781 agtttggaaa actagaattt ttatcagtat tgttacaaga ttataaagaa ataagaagat
      841 tgtgtaaaga gaataaattc tgtttttata gtacttcaaa taaagataat tttttagttt
      901 ggagaagtat tatatatatt aatacagtta ttttgaatga taatattgat gaattaatta
      961 gtctagtatt acaaaattca aaagatacaa atattgatgg tttgcttaag gatcgtactt
     1021 caatgaatat attacatttg aattctttat attataatat gttgatatat cataaaaata
     1081 aatgtgcgaa tatgatagaa aatatgaata ttaatcaatt aagtattttt gctattaccg
     1141 caacaaaccc agaatgttta aataatataa tgaaggataa atcattaata gaaaaaataa
     1201 agcaagtact tgaagatgag attagtaatg aaaacttaag tggtgttatt tgtttggcat
     1261 taaagaagtt tttaggaggt tctttagtaa agaaagaaga agagcttgtt aagaaatatc
     1321 ttgaaaatat agaatttatt ggatcatcga tattctggaa ggggcactgt gatataagcg
     1381 cctttatatt atctgttttt ggaaaaggac aaatcaagtt aaaaattgat caagaaataa
     1441 agagatatgt aaatgtatat aaagtttttt tagaattaat agataaatca tgttcaattg
     1501 aaagatttat atgttgcgtt caaaagaata ttttcaacaa tacgcaagat tcttattata
     1561 taagaatttt gctaggaaaa gtattgtcat tacttaatga tgaagatttt aatataaaag
     1621 gatcgcttga ttatttaaat gattctatta atgatagtag aattttaatt atatattgta
     1681 caatgaaatt atataatcca tctagattca aaagtttatt aagtataact gaacttacga
     1741 aattaactga acctagagta tatgaagatg ttaattttac tagtacgagt gaatcacttt
     1801 ttatgctatc atttattttg tctaatcatg ataaattact aggacatgaa ttacttttaa
     1861 aaggaattag taatgggata atgaggatga atgatagaaa ggatacaata ggagactatc
     1921 agctactaga tagcttagag atacttctca taaataattg gatttcaacg gagaaattaa
     1981 ttattttgct aaaaagaata ttaaaaatta caaacaaaat gaatttatat aatatagaaa
     2041 atgatactca tgcaagagta atggatatta ttcaaaaata tgattttgca gctgctgaat
     2101 tttattataa taaaattgca catttagaag agtgttataa tttaatacat tatagatatg
     2161 cactatcaat gattcagaga ggcaggaata taaaatatat agaagcttgt ttggacaata
     2221 ttaaattatc ttttgataga tatcatcaaa aggcggaatg gggaagtttt tactataaaa
     2281 tcgaagtata tttaaagata tcagcaagcg atttttattc atcatatgaa caaaagcttt
     2341 catttgaaaa agcatgttat aaaattgatg aactagaaca tgctggttgg agtagagaac
     2401 ttaaaaaaga agaatatgat atttatgttg aactatgcaa aaaatataat aaaaaaattg
     2461 atattgaaaa agaaaaagaa aatgagtatt gctattcagt taaaccttta gaaaaagaaa
     2521 accaagatat tcttgaaatt attaattcta ttaatacaga agaagattta tgtgaattta
     2581 taaaaagact agatggagaa tactatcttg aaaacttaga aataaatgaa ttgtttatta
     2641 aaaaatgtgt agatattaat ggaaatattg aagttattat tgatatgttt acaagatata
     2701 attatccaag tagtttgtac tattcacgaa atagccataa tttctggatg acagttctat
     2761 cagctctaaa aaatgcaaag tctaaaaata gtatgatgca atatttatta gagaatggcg
     2821 gaggacatga tgggtttagt gaattaataa aaatttatgg atatttagga aataaggaca
     2881 tatgtattaa attatttgaa agaatgttaa cctgtgttga atttttatta tgctaaaata
     2941 aatttttata attatgtcaa ttgacaaata tttcatgtgg atggaagagg gtatatcctc
     3001 attgggaaat gatggtatat taattggata tataaagagt ttaatattta ttgtaataaa
     3061 gtaaaaatat gaaatattaa tttaaattgg agtatcgata ctgtaaagta tcgatgcttt
     3121 tatatatttg aataatttaa gctataaaac ttaaaacttg ttattgcaag ttatataaag
     3181 tgggctagtt atgaaataac tgttatttgt agagataatc tatactcctt ttttttctaa
     3241 atggacatta gtataataat attagaggtg gtagaagaaa tgaaaataaa tgattttata
     3301 tttagattta agtatagaaa tgatgaaaaa agtttatgta gagtaagaac attcttagga
     3361 agagagaata aaataattaa cttaataaca gatttagaag atttaaatat ctcaaaagta
     3421 agtttaatta attccattga atttgcttgt tctgaattga tagaaaaaaa tatgacatgt
     3481 gaagatgcta tatttataga acatacagag tcagaaagtt tagaagaaca gaaatttgat
     3541 atggtaacct ttaatcctag aggaggacct atttggaaaa atatcaatat aaacgaagta
     3601 aaaaaaattt taaattgtat tgatgatgaa cttataaata caacaaaaaa gaataggaat
     3661 ttagttgaaa caatagatag aattagatat agaataaatc ctgaaactga ttttttatat
     3721 aaagaatcgc cagaagtaat taagcgaaaa ttagaaatta aagaaaatag aattagtaag
     3781 agtaaactag aagatttaat aaaaaataga tcaaaggagc aagaaatagc tagattattg
     3841 aaatcagatt tatctgtatt tggagaagta tattcttttc ctaaagatga gtatatatgt
     3901 ttttcagagt atccaattta tgataggtat atagattatg ttgttataac cggaaggtca
     3961 agaatgcaag tatatttaat tgaaattaaa ggagctgaat ttaatttagt taataaagat
     4021 agcaaattta atgagaagat taatgaagct gacagtcaaa tatctagaac tttaggatat
     4081 atatttagaa attatatggc gtttaaagat gatatacacg aaaaaagaag attggttgaa
     4141 aaaggagaga aaatattcaa ttcatttaga gggccaatta gcaaattaga agttgataga
     4201 caaaaagata taactattca ctcagtaata ataggtggag taacaaatga tgatattaaa
     4261 gaaagtagat taagacatga ttatgaaaat aagaatgact tcaggataaa actagaatca
     4321 tgggatacat ttataagaaa attaagtaat agatagtttt tagatattaa aaaattaatt
     4381 aactataatt actattaagt tttttattta atttgatagg cataaaaaga atttaatcgt
     4441 taaatatgtt tagaataggt caatagagtt agacaatgtt atatgtaatt agtagggaag
     4501 ggtaatatta ggaagattag ggcccttcct ctttgatgtt gttatgatta attatataca
     4561 attaaaatat ataatagaag atgtttatag gtaattatac tactactgtc tgtgaaactt
     4621 tatatattgt tactatatat ataacaaatt atcttatata aggttatata tagtatagat
     4681 gtatctat
//
LOCUS       DAGSWI010000209         4675 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.214, whole genome shotgun sequence.
ACCESSION   DAGSWI010000209 DAGSWI010000000
VERSION     DAGSWI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.214"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            163..1314
                     /locus_tag="MJF80_003677"
     CDS             163..1314
                     /locus_tag="MJF80_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBZ0282282.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1360..2535
                     /gene="thiI"
                     /locus_tag="MJF80_003678"
     CDS             1360..2535
                     /gene="thiI"
                     /locus_tag="MJF80_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBZ0282283.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEETTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2671..3474
                     /locus_tag="MJF80_003679"
     CDS             2671..3474
                     /locus_tag="MJF80_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBZ0282284.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKDKNEVIKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3717..4358
                     /locus_tag="MJF80_003680"
     CDS             3717..4358
                     /locus_tag="MJF80_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBZ0282285.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYSAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
BASE COUNT         1875 a          451 c          904 g         1445 t
ORIGIN      
        1 ttactataaa aatttgtaat aaagtgaaaa aactagcttt tcatgaaata atattttata
       61 atttaagatt gtctttttaa taaagattaa caaaaaaaat actataatta gtggtataat
      121 agttagagtt gaagatttta aataaaggta ggtgtaatta agttggaaat atacttagac
      181 aatagcgcaa ctacaaaacc ttatcaagaa gttatagata aaatggttta tgcacttaat
      241 acggaatatg gaaatccttc ttctgtccat agaaaaggtg tagaagttga aaaagctata
      301 aaagaagtaa gacaagatat agctaaatct ctaggagcga aagaaaagga gatttatttt
      361 acatcaggtg gtacagagtg taataatact ataataagag gtattactag tttaaataaa
      421 aaaagaaaaa atcacataat atctactaac atagaacatc cttctgtttt aaatacatta
      481 aaagatttag aagaagatgg ttttgaagta acttatttag aggtcggaaa agatgggaaa
      541 ataaatatag aggatttaaa aaatgcgata aagtcaacta catgcttagt aagcatgatg
      601 catgttaaca atgaaatagg aactatacag ccaataggtg aagttggaaa gtatttgaag
      661 ggtttaaaag aaaaaattta tttccatgtt gatgctattc aatcatatgg aaaaattaac
      721 tttagaccat ctaaatacaa tatagatttt atgagtgtaa gtgcacataa atttcatggt
      781 ccaaagggaa ttggatttat gtatataaaa gaaaataatc gccttaagcc tatgttaaca
      841 ggtggtggtc aggaaattgg aattaggtcg ggaacagaaa atgtacctgg aatatatggt
      901 cttggagaag ctgttagaat actaaacaaa gatttagatg ctgttatttc gaaagtagat
      961 aacttaaaaa atatattaaa aaatgaaata attgataata tagaagatat aaaaatcaat
     1021 tctccagaag atggagtatg tcatatatta aatgtatcat ttagagggac aaaaggagaa
     1081 gttttacttc attacttaga gcaaaaaggt atttatgtat caactggctc tgcatgttct
     1141 tctaaaaaga aaggtagtta cgtattaaat gctataggtc ttacaaatga agaaataaat
     1201 ggaacaataa gatttagttt atctgatatg aatacagaag aagaaatgtt agaagctgtg
     1261 aaagttttga aagaatcaat ttgtgactta aggtcaataa tgaaaagaaa ataaagaata
     1321 ctgtataaat agtgataata aattagttta agaggtgaaa tggtgtataa tatattaata
     1381 gtaaagtatg gagaaattgg agtaaaagga aagaatagat atatatttga gaataggctt
     1441 ataagaaata taagaaatat gttaaaacca attggaagat ttaatgtata taaagagtat
     1501 ggaagaatat atgttgattt agaagactat gattatgaag aagtaattga agaagttaga
     1561 aaagtatttg ggatagtggg tgtgtgccct ggagtaagag ctaaaaaaga ttatgataca
     1621 ttaaaagaaa tagctttaaa aatgttagaa gaaaaaatag aggctggata taaaactttt
     1681 aaggtagagt caagaagagg agataagtct ttcggattga cttctcaaga aatgagtatg
     1741 gatataggtg gatacttatt atcaaaggta ggagatagga ttaatgttga tgtaagaaat
     1801 ccagaagtta aaataaagtg cgaatacaga gaatttcata ctatggtcta tagtgatact
     1861 atacctggat atggtggatt gccacttgga acgaatggta gagctatgtc tcttttatca
     1921 ggaggtatag atagcccagt agctacatgg atggttgcaa aaagaggtat ggaagtagag
     1981 gcagttcact ttcatagtta tccatttaca agtgaaaggt cacaggaaaa agttaaggat
     2041 ttagctaaaa tactggcaaa atactgtggt agagtaaggc ttcataaagt taatatctta
     2101 gaaatacaaa aagcaattgg cgagaattgt aatgaagaag aaactactat tctttcaaga
     2161 agatttatga tgaggatagc acagagactt tctgaaaaaa gacattgtga tgctttaatt
     2221 acaggtgaaa gtattgggca ggttgcatct caaactatac aagggcttac ttgtactaat
     2281 gccgttgtag acttaccagt gtttagacca cttatagcaa tggataaatc agatatagtt
     2341 gatatagcca aaaaaatagg gacttttgaa acatctatag tgcctgaaga agattgttgt
     2401 agtgtatttt ctcctagaaa gcccgtcact aaacctagat tagagaaaat agaaaaatct
     2461 gaaacagcac ttgacattga aaaattagta caggatgcta ttgacaaaat agaggtagag
     2521 gatattgagt tttagcatac gagttattta attactaaat tgaatattga gtatattagt
     2581 agttctgttg aaaggattta tgtagacaat ttattaatat gtagtattta aaaataagta
     2641 ctataatttt atttaatggg aatgattatt atgaataaaa agggttttac attaattgaa
     2701 ttgttggtag ttatatctat aataggaatt ttagttatag tagctgttcc agcgttattt
     2761 agaaatatag aaaaaagtaa agcagttaca tgtctttcta atagagaaaa tataaagact
     2821 caaattgtta ttgcaatggc tgaggaatca agtaaagaca agaatgaagt cataaaagag
     2881 gtattagaaa acaaagatgg taagtacttt gaaacagaac caaagtgtaa gtcaggtgga
     2941 atatattcag caacgtttga tgatggttat gatggaataa ctggaataga aagcattgca
     3001 aaagtgtatg ttacctgtac aaaacatcca gacggtgttg aaatggctag ggatgtacat
     3061 caaagtatga aggatttgat tgcatcattt gcacaagacc cttctataat accaggagct
     3121 tcaaagggca atgatgattt tagaaaatat ttattagaca ataaatataa aaatgggtgg
     3181 cctacaattc cagatgaatt taaggcaaaa tatggattaa gtaaggatac actatatata
     3241 caaccatatg catataatcc tactaaatct gatgctactg tagttgtatt tgcaaataat
     3301 aagactggag gtaattggta tacttcccta gtttacgatt atgatgaagg tagatggtat
     3361 aaaggtaaaa atggtatttc tgttgcaggt aggtcatggg atgttgacac agatagtgtt
     3421 aagtctgtaa aaacagagat tcattctaaa gagggatggg gtcctttaaa ttaatataac
     3481 atgttttatt aatctggtat tgattaaact atagaaaaga gcagtttcta tagatgacga
     3541 ataccagatt ttcatttata tatataattg agatatatta aaagtgttta tatttttaag
     3601 tcttcacata caagctaaaa ataattataa taatattgta aagaaattta attataagat
     3661 taattttctg aagatttagt atttgtacta agaaatacat aaaaagggga atagatatga
     3721 ataataattt tcaagacaac caaacaataa acttaataca aagtcgtaga agtatacgaa
     3781 aatttacgac agaacaaatt agtgatgagc aagtaaacac attattacac tgtgcatttg
     3841 cagcaccttc aggatgtaat aaacagcctt ggcacataac tgttgtgcaa gaccagaaac
     3901 ttttaaaaga gattagtgat gatactttat ctaggattca tgaggtgagt aatgttgaga
     3961 taaataaaaa ctttaagtta ttttatagtg ctccaaccgt tttatttatt tcttatgatg
     4021 aaagtagttc atgggctcca tatgatatag gaatacttac agggaatata acaacagctg
     4081 cacaagcact agggcttgga agttgtataa taggaatggt tagaggttta tttacacctg
     4141 ttgaacaagg ggatattgaa ggtcttgtga gtgtattgga taaagaagat gtaaaagaat
     4201 cagagtcaat aaagatgaag tttgatacaa ataaaaaata cagagaacta cttgatatac
     4261 cagaagggta ttctgtacca tttggtattg ctgtaggtat tccagatgga aatttaccaa
     4321 atgctagaga ggtagtttat aaagtttcca gagtttagga taaagtattg caagaaacaa
     4381 tcagttactt gaagaattaa atcagtaata tacaaataaa ttgatggaag aggaagctat
     4441 ttaaaaagta gtttagattt ttcgttgtaa aataattaaa taaagaataa ggtgataagt
     4501 attttattta ttatcttatt cttttttatt tttgttttgt agaatgataa aaataaggat
     4561 taagtgtaat aaaatagaaa acaagtatgt taattttaag tatatgcttt aaaatttatc
     4621 caagttaaaa atttaagata tcttttcagt ataatggaaa aaatgtcaaa atatt
//
LOCUS       DAGSWI010000210         4662 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.215, whole genome shotgun sequence.
ACCESSION   DAGSWI010000210 DAGSWI010000000
VERSION     DAGSWI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.215"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(135..911)
                     /locus_tag="MJF80_003681"
     CDS             complement(135..911)
                     /locus_tag="MJF80_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBZ0282286.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLIDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(968..1939)
                     /gene="asrC"
                     /locus_tag="MJF80_003682"
     CDS             complement(968..1939)
                     /gene="asrC"
                     /locus_tag="MJF80_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBZ0282287.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(1952..2743)
                     /gene="asrB"
                     /locus_tag="MJF80_003683"
     CDS             complement(1952..2743)
                     /gene="asrB"
                     /locus_tag="MJF80_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBZ0282288.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(2744..3751)
                     /gene="asrA"
                     /locus_tag="MJF80_003684"
     CDS             complement(2744..3751)
                     /gene="asrA"
                     /locus_tag="MJF80_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBZ0282289.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            complement(4142..4483)
                     /locus_tag="MJF80_003685"
     CDS             complement(4142..4483)
                     /locus_tag="MJF80_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBZ0282290.1"
                     /translation="MQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
BASE COUNT         1508 a          892 c          421 g         1841 t
ORIGIN      
        1 tatatataac cgatatattt tcgtatgctc ccatattttt tttgtttcaa atattttttc
       61 aaaaatataa aaataactct ctcaacaata ctaaaaatgc atttatcaag agagttataa
      121 tttatacaat aaatttaatt aagcttttct ttacctaata tataagttcc aagacccata
      181 aataaagctc ctccaacaat atttccaagt gttacagcta ctaaattata aatagctcct
      241 ccaatagtaa ctgtatttat tgttggtgaa aataatgaaa cactataaat tgtcatattt
      301 gctacactat gctcaaagcc tgatgttata aaagcaaata aacataagaa tatcataatt
      361 atttttgcag tatcactatt agttctaaac gaacacaaca ctgatacaca tactagaata
      421 ttacataaaa tccctctaaa gaaaagagct gtaaatggca tggatgcttt actagctgct
      481 gtatttgcaa aaaattcagc tacaggtcct ttgtctatta gaccagttcc tacaaatata
      541 atgcctaaaa ccaaagctcc gattaaatta cctacccaac tgtatgccca tattttagaa
      601 gtatctttta tacttacacc tttatttaac ataccagcag acataaccat attgtttcca
      661 gtaaataact ctgttcccgt cattataacc aaacttagtg ctattgcaaa tgataacccc
      721 attactatct tagtcatagg tgaacctgca tcagtcaaaa gtcctccaat agtaaatatc
      781 aaaagtatac ctatacctac ataaagtcct gcaaacgcag aagataccaa atattttact
      841 ttacttgtgt ttaataaatt tatcttattt atagctgcat tagttaattt atccaaagtt
      901 tctttatgca taattccctc ttccttctat taaattttaa tttattcaaa tatatgtttt
      961 actcaattta tagatattta attccactcc agtatatgtt ttcgtacatt ttagttattt
     1021 ctggaagctc tacaccttca agtgcccatt ttttaaattc cataaatcct gttctatcaa
     1081 ttatatatcc tatatgttct tttcctcctg gtgcatcttt atctatatac ttttctacat
     1141 atctataagt attaagtatt atctttatta tgctgtcctc atctacccat aaaagaaaat
     1201 cttctgctag tctagggttt ttctttcctg ttctacccat tatagctaat ctatagtatt
     1261 ttttctcatc tctagtccat gcatttgttg ggcaatttag cacacactct ccacagccta
     1321 tacacttatc tttatctctt accaccttat tattttctgt ttttaatgct ccaacagata
     1381 tcttagaaca tttttttaca catgctccac aatttacaca tctatccttt tcatatattg
     1441 gtaaagccat accaataatt ccaaagtcat tcattctagc ttttatacaa tcatttggac
     1501 aaccagttaa agccacttta aaatgtaaat catttggaaa tatagctttt tctattcttt
     1561 tagcaaattc agttgtatta tattgagcct ttggacagac tttatttcct atacaagctg
     1621 ctatatttct tgtacctgct gctggataac ctgcgttttt ctcactttga tttatattca
     1681 ttttttcaat aacaggttgt ataattttat ttatttcttc catatcttcc atatttattc
     1741 ctaatatttc aaacccctgt cttgtagtta tgtgcacctg tccattgccg tactcacttg
     1801 caattttaga aactacagct aaactctcag ggtcaataag acctccaggt actctaactc
     1861 ttaaagaagt ctcatatttt gtttttgtaa ttctataagc atttttcatt acttttttgg
     1921 tatttaaatc tcttatcaca aacttcaccc cctaatcaat aagattttta gcatatgaat
     1981 aatcaaatac tggtccatct aaacatatgt aagtatcatc cattttacaa tgtccacatt
     2041 tacctattcc acaacacatt tttctttcat aagaaaccca aatgttcttt tcatctaaat
     2101 ctctttttaa aaattcttct actgtaaatt tcatcatcat tggaggtcca actacaacta
     2161 tagaagtatt gtttatatct ttcatatcca attctggtat gtattttgtt actaatccta
     2221 cattaccact atatccttcc tctgctccat ctacagtaac taaaatatca agttttttac
     2281 tccatctctt taaatcatct gcaaataaaa tatcttttgg tgatttaaat cctactatta
     2341 atttaaactt ttcgcatttt tctttattat tataaaaata ttcaactatt cctcttacag
     2401 gggctaatgc acttcctcct gccactacta caatttctct tccttcataa agggatacat
     2461 caaatccatt tccataagga cctcttataa aaaaagaatc tccttcttta taattaaaca
     2521 actcgctagt tacttttcct acatttctta ttgtaaagtc aatataatcc tctccatatc
     2581 ctgatactga tattggactt tcaccgtatt taggtataga tatctcataa aactttccag
     2641 gcaatacacc ttttgtattg cagtttactc taaaagtcca ttctttatca gtatgtttaa
     2701 ctatatctag tatttttgca cttactggta tatatgggtt catttatact tcctccttac
     2761 taattactaa atcatttatt ttttctatac actttgaaaa tgatatgtat tgcggacagg
     2821 catcatcaca tcttccacag ccaacacaca tatgatatcc gaatcttttt ttaaaatcat
     2881 gtattttatg catagtttta aatctcattc tttcgccttg tttttttcta aaagaatttc
     2941 cacctgctat atctgtatat ccatctactt gacaagaagc ccaaaccctt cttctctcac
     3001 ctacattttc gttttcctta tagaaaatat cctgcattgt aaaacatgaa catgttggac
     3061 atacaaaatt acatctacca catcctatac atcttgtatc atattcatcc catattgaat
     3121 ttcctatcat ttcatttgaa tcaatattct ctggcacatc tacagatatt aaattatcag
     3181 ttacataatc aacattaaat tcagttgctt ctccattaaa tacttcaaat tcactatcct
     3241 ttatatctaa gtacagtgta tctccttcta tatttaatcc aattgaataa ttatcagttt
     3301 tgttagaatc catgctcaca caaaaacagt ttctaaaact ttccttacac cctacaagtg
     3361 caaactttac tttatctctg atatttttat aaaaataatc tttttcttca ccattattta
     3421 gatatatttc atcaagtctt tttataccat ttatgtcaca agctcttaag aaaattaaaa
     3481 ttttcttgtt gtctaaatcg ctttccttaa actctttttc agtaaaataa aacaatactt
     3541 gatttatggg aagtacaact tcttttggtg aaaagtttgc tttcttagca aattccattt
     3601 cttcaaaagc gtttacctcc ttgtatctta taacatcagt atctgaaaaa gtacctttaa
     3661 atgggattaa aactggtgca aatatcttgt agtctttttt tagataattc agaccttcat
     3721 tgaacttaga tgaatctaat tttagtttca tttttatcac ctttcttata ttttagtttt
     3781 acttacttgc tatcttgttt aaatcttaac actttatttt ctatgcttcc gtgataatta
     3841 tcacagtttc aaaaataata aaaaaaatat taatggagtt tattaagttc tgttaatatt
     3901 tttttattat ctacattatt cgatttcaaa tctaatcact taaatacaat cattaagtac
     3961 aataaaacta ctatctactc aattttttga ataaaaaaga atcttatttg tttttttata
     4021 caattcaata gggtaaaata aaaattaata aattagacaa taaaaaagaa tctataaata
     4081 gattctctta ataatatttt tatttaatta aaattctaat aatactctta cttaatcata
     4141 attaattatt tttaaaatac cttgataatt cagtttcttt aacataaaaa cctttattgt
     4201 caattttaat aagttcttct ttttgcaata cctttatcat tctggaaaca gtctcccttt
     4261 tacagccaag catatcagct aaatatgtaa ctgtaatatt tacatttagt aaagtccatt
     4321 ctccacagca aactccaaat tctctactta acctatataa ttttgctgca agttttttat
     4381 ctacttttat ggatatagaa tttttcaact gtctatataa acgtcttatt cttctttcca
     4441 tataagaaat aatatccttt gtaagttgaa aatcattttg cataatttct aaaaattctt
     4501 caatactata ataagctaca attgaatttt caaatgcttc acagccaaca gtagagtttt
     4561 tagaagcgtc aagtgtaata tcatttatga cttccccact atttaatatg aaaattactt
     4621 ttctctctcc attttcactt atcttaaaaa tagatacctt tc
//
LOCUS       DAGSWI010000211         4607 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.216, whole genome shotgun sequence.
ACCESSION   DAGSWI010000211 DAGSWI010000000
VERSION     DAGSWI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.216"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            231..1310
                     /locus_tag="MJF80_003686"
     CDS             231..1310
                     /locus_tag="MJF80_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBZ0282291.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIVKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="MJF80_003687"
     CDS             1634..2002
                     /locus_tag="MJF80_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HBZ0282292.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2916
                     /locus_tag="MJF80_003688"
     CDS             2119..2916
                     /locus_tag="MJF80_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBZ0282293.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQL"
     gene            3315..4205
                     /locus_tag="MJF80_003689"
     CDS             3315..4205
                     /locus_tag="MJF80_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBZ0282294.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKEIIKAYKRSESYIQIKFF"
BASE COUNT         1753 a          496 c          865 g         1493 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagccttta
      601 tcatcaagga cgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggct gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacccg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgtaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctac taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagct atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttagcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaaag gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatactttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgagcactat ggaagaaaaa tacatcttat ctgaccgagg taaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttagtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttataatata taaaaattac atacccatta
     2941 ggtaaattat gtagtatatg tgtttacaga tggttataga aataaatatg taataaacta
     3001 cttaacactt aaaaagtgga tttgtccaga gtataataca aagcatgata tatataaatt
     3061 tttaagcgaa atacatcaaa agaaggatta gaattagtgg agacagaaca gctattatag
     3121 attataaata aatagtttta aaggagaggt actacgaata tagaggaagg taaatcatac
     3181 gttattcaag tattataaat gattagttta aaagtactat cgaataggag tctccatatt
     3241 taaaccttag taaaggtagt taaagtagta gtttaaaaac taaaaaatta tataaaggaa
     3301 ttaggtataa gaaaatgatt gagagtatac cagcaaaaca aattttacaa aaagtgaaat
     3361 ttgacaatac aagatggttt ggaattgact ataatatgaa tctctatcgt ggttgttctc
     3421 atggatgtat atattgtgat agccgtagca caatatacaa catagagaat tttgacaaag
     3481 ttagatataa agaaaatgtg atagaaattt taagtaaaga attgcgttca aaaagaaaaa
     3541 aaggggtagt aggcattgga gctatgtctg acacatataa tccttttgaa aagcaacttt
     3601 gtattacaaa gcaggcacta gatttgattt cggaaaatca ttttggagtg tctattgata
     3661 ctaaaagtag tttggtggtt agagatatac cgattttgca gaaaattaaa aaaaataatt
     3721 ctgctattgt aaagttgact ataaccactg caaatgatga attatcaaag aaaatagaac
     3781 cttatgtaaa tccaagttct gtacgatttg aggcagtgaa agaacttaat gattctggaa
     3841 ttttttgtgg tatattattg actcctatgc ttccattttt gactgataat aaagatgaaa
     3901 tacgagcaat tgtagaaaaa gcacacaagg ctaatgcaaa atttatttat tgcatgtatg
     3961 gtgtaacaat gcgtagtggt cagagagagt ttttttatga gcatttaaga gatatatcac
     4021 caaaattggt acttaaatat cagaaaacat atggtttaaa ctatgtatgt actgtacaaa
     4081 ataaagattg ttgtgagaag ttgttaagag aagaatgtgc acgttatggt attttgacgg
     4141 atatgaaaga gattataaaa gcgtacaaga ggtcggagtc atatattcaa ataaaatttt
     4201 tttaagatag gctttggatg agatataata gtggtgattg atgtgttgat attatagttg
     4261 attgttagag aggatattat agatgaatat tagtattgat attttaggta attgctagag
     4321 ctgatattat agatgaatat tagtattgat attctaggta attgctagag ttagtattat
     4381 agatgaatat tagtattgat attctaggta actgctagag ctaatattat agatgaatat
     4441 tagtattgat attctaggta attgctagag ctaatattat agataaatat tagtattgat
     4501 attctaggta attgctagag ctgatattat agatgaatat tagtattgat attctaggta
     4561 actgctagag ctaatattat agatgaatat taatattgat attctag
//
LOCUS       DAGSWI010000212         4595 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.217, whole genome shotgun sequence.
ACCESSION   DAGSWI010000212 DAGSWI010000000
VERSION     DAGSWI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.217"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(441..2828)
                     /gene="cadA"
                     /locus_tag="MJF80_003690"
     CDS             complement(441..2828)
                     /gene="cadA"
                     /locus_tag="MJF80_003690"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBZ0282295.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="MJF80_003691"
     CDS             complement(2851..3216)
                     /locus_tag="MJF80_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0282296.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="MJF80_003692"
     CDS             3415..4263
                     /locus_tag="MJF80_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282297.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
BASE COUNT         1567 a          798 c          498 g         1732 t
ORIGIN      
        1 cttaaaaata attcgtcaat actttttaaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaacgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatacttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaaaaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctagaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgttaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggctctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcatgcc taacaatgtg cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggca tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctgtac aaggtatacc acaagctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtgttgg tgattcagaa
     4501 tagtatggta ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attga
//
LOCUS       DAGSWI010000213         4592 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.218, whole genome shotgun sequence.
ACCESSION   DAGSWI010000213 DAGSWI010000000
VERSION     DAGSWI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.218"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(64..348)
                     /locus_tag="MJF80_003693"
                     /pseudo
     CDS             complement(64..348)
                     /locus_tag="MJF80_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            556..1374
                     /locus_tag="MJF80_003694"
     CDS             556..1374
                     /locus_tag="MJF80_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0282298.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(1507..3627)
                     /gene="feoB"
                     /locus_tag="MJF80_003695"
     CDS             complement(1507..3627)
                     /gene="feoB"
                     /locus_tag="MJF80_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBZ0282299.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKENQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(3653..3880)
                     /locus_tag="MJF80_003696"
     CDS             complement(3653..3880)
                     /locus_tag="MJF80_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBZ0282300.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(4206..>4592)
                     /locus_tag="MJF80_003697"
     CDS             complement(4206..>4592)
                     /locus_tag="MJF80_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0282301.1"
                     /translation="AKSRYEVFACEQKIFSLNDYDDIDKSSYHIFLKENGLICAYARI
                     IPKEYSSYNDVYIGRVLVLSSHRRKGLAKQMMDCAIDFIKVNLHENNITLSAQTYIKN
                     LYLSCGFKEISEVYDEAGIEHIKMRL"
BASE COUNT         1712 a          665 c          590 g         1625 t
ORIGIN      
        1 agaaatccta ttaccttttt attattaatt ttatcagtgt tttattataa atagcttaca
       61 taattacaca ttttttctga ttaaataata tgatgctatt gttataatag atactaatgc
      121 taatgatgtt attgcagtat ctaatcttcc tattaataaa ctcatttcta tatattcaag
      181 ttctattaaa tattaataaa atatttctag tttaacagct ttttctctat ctatatttcc
      241 atactttaac tctaatttat ctaaagattt tttattacaa taattaactt tatgtttaat
      301 taaatatctt attgtagcac ctattacaat tttcactcta acaagcataa atataatatt
      361 ccatccaaaa gttaagaggg tatatctttt tttatgatgg gaatgggtaa aatatttata
      421 actgacatct aactgatatt taagctttaa aatcaaaacc atattgattt tctaaaataa
      481 taactacttg atttagtaaa taataaagaa tatagtaact atatagacaa caaaaagaaa
      541 aggagtgtat aacccttgga aaaacagtat tttacaacag gtgaatttgc taaattgtgt
      601 ggtatatcta agcagacatt aattttctat gacaaaatag gtattttttc tcctgaatat
      661 aaagataaaa ataattatag atattactca gtttatcaat atgatacact tgatatatta
      721 caatcattaa gagaaatagg aatgagttta gaggaaataa aagagtatat acaaaataga
      781 actccccagc tttgtgtgaa aatgcttaaa gaagaagaaa aaaagataag agaaaaaata
      841 aaaaaactaa gaaagataag ttctaagatg caaaatagaa taaatataac agttgaagga
      901 atttctaaaa tgaatagtga agaaatttat atatccaaaa agtttgaaga atatttagtt
      961 ctatcaagcg atttgagtaa tatgagtaat agtgatttta tgatggaatt aatagatttt
     1021 accaattatt gtaaatcgaa aaatttgtat caaggatatg agttgggagt aatagtaagt
     1081 aatgaaaata taaaaaatgg ggactattta agtatatcaa gtttttattt gaagatagat
     1141 aaaaaaataa aggataaaaa gctatatgta aagccagaag gaatgtatgc ttgcataaaa
     1201 catattggaa aatatgagga ttcatataaa tcttacgaaa aattgaaaaa atatatatat
     1261 gaaaatgaat ataaaattat tggaaattcg tatgaggaga gtatacttga ctttttttgt
     1321 gaaagtaatg aagataatta tatgactgaa atttcaatcc aagtatccag ataagaatta
     1381 aaatagagat atattcaaat gttaagtaat tttacttatc attgaatata tctctatgct
     1441 ttataataaa gtatataaat ttttaaatag tttctctaat atatgtctac tgtcagttaa
     1501 tactaattat ataaataaat tgtaaactgt agttgttaaa aaacaaacaa caaaagcaag
     1561 tatagtaggc attaagaatc ctaaggcagt ccattttaaa ctaccagttt ctttttttat
     1621 tgttaaaagt gttgttgcac aaggccaatg cagtaaactg aacagcataa catttaaagc
     1681 tgtcaaataa gtccatccat gttctctaag aacttgacct aaagcactaa agctatctaa
     1741 ttctatcata gaaccagttg ctaggtaagc cattaaaagt ataggtacaa ctatctcatt
     1801 tgctgggaat ccaagaataa aagcaagtaa tataaaacca tcaagaccaa ttaatttagc
     1861 taaagggtct aaaaagtttg caacatgtga taatatactt aaatctccta tatatatatt
     1921 tgcaaatatc caagttatga caccagcagg tatggctacc attacagccc ttcctaaaac
     1981 aaatattgtt ctatctatta tagaggtata taaagttctt cctatttgag gaactctata
     2041 aggaggaagt tctaatgtaa atgtagacgg aactcctttt aatagcgtct tagaaagagt
     2101 gtaagaaacc aacaatgtta ttataacacc tagaattatc agtaaagtta tgcaaagtgc
     2161 agttgctaca cttgatacga aagagttggt tataactgag ctaaaaaata ttgtagatat
     2221 agctatcaaa gtaggaaaac gaccattaca aggaacaaag ttgtttgtaa gtatagctat
     2281 aagtctttcc cttggagagt caattatcct acaaccaata actccagctg cattacatcc
     2341 aaatcccata cacatagtca aacactgttt tccatgtgca caagcttttt taaataggtg
     2401 gtctaagtta aatgcaactc ttggcaaata tccaaaatcc tcaagtaagg taaataatgg
     2461 aaaaaatatt gccataggag gtagcattac agagattacc catgctaatg ttctgtataa
     2521 tcctaaaacc aacatatcat ttaaccatga tggacaatta atactattta atatacccga
     2581 aatacttggc tcaaatccga gtaataaatt agataataaa gtggatggat agttagctcc
     2641 ttctatagta atccacaata tagtacctaa taataagagc attattggaa gcccaaatat
     2701 tttagatgtt aatattttat ctactttttc ttctctatca gttgattttt tagcaacatt
     2761 agaacaacac tcatcactca gttttttagc ataatcatag ttgattttag taaattcatc
     2821 tcttattttc tgtttattta tattgtctgg aatctttttc tttacttcat taatagcatc
     2881 tatagaatct ttgtctatat aattactcat agattcaaat atactttcat cgccatcaat
     2941 aagtctaagt cccaaccacc tagaatttat acctggtata atattatcca attctggttg
     3001 aattgatttt accacattct ctatattttc attgtatcta acaggtttat tatttaattt
     3061 atatttgtca aaagatacat catttaaagt atccaatagt tcatccatac cagagccatt
     3121 tcttgcagca gttaaaataa ctggaatacc aagagaatcc tctaaaagtt ttttatcaat
     3181 agtaatgcct ttttttctag cttcatctat taaattaata cacaaaataa ctttatctgt
     3241 aagttccatc acttgaaata ctaaatttaa atttctttca agacatgtag catcacatac
     3301 tactattact gcatctggat taccaaaaca tataaaatca cgagctacaa tttcttcttg
     3361 tgataaggca aataaagaat atgttcctgg taaatctatt aatgcatatt cggtattttt
     3421 atatttaaaa ttaccacgag ctgtagctac agtttttcct ggccagtttc cagtatgctg
     3481 tctaagccct gtaagatgat taaatacagt acttttacct gtatttggat tgccagccaa
     3541 tgcaattaca aactggtttt ccttattgtc tatatcaaac atgtctttta aagaactcat
     3601 tttagttgag ttatgtgtca accccatatt aatatcctcc tgctagaaat ttttaaatgt
     3661 cagaaactaa tattaagcta ctttcttctt gtcgtagagc tatcttgcta cctctgattt
     3721 tatagacagt taaattattt tttggacctt ttctaactac atctataaca gctcctctag
     3781 taagaccaag cgctaacatt ctttctctta aattaccact agataatata tcttcaactt
     3841 tgacagtttt tttcacttga atatcgttaa ggtttttcat aatagcacct ccatgcttga
     3901 aatttagcct gggctaatct tcttatttta aactatgcat tatattatta ccttgttact
     3961 tttacaaaaa aagataggca agatgcctat catacaaagt tgggtataat tcttactttt
     4021 ggggaaagta gaatatagaa taaaaattaa cactttgaat ccatataatt attgaatttt
     4081 gataataaaa aagtcagaaa gcaaaagcct tctgactcta tgtaaggtat tttcaaatac
     4141 ctaaatatca attcaatatt taatttatac ttaaattata tattaacaat attagcttgt
     4201 caacactata atctcatttt aatatgttca atacctgctt cgtcgtatac ttctgagatt
     4261 tctttaaaac cacaagataa gtaaaggttc tttatataag tttgtgctga taaggttata
     4321 ttattttcgt gtaagttaac ttttataaaa tctatagcac aatccatcat ttgttttgct
     4381 aatccttttc ttctatgact agaaagaact aaaactctac caatgtaaac atcattataa
     4441 gagctgtatt ctttaggaat tattctagcg taagcacaaa tcaatccatt ttcttttaaa
     4501 aatatgtgat aagagctttt atctatatca tcataatcgt ttaatgaaaa tatcttctgt
     4561 tcacaagcaa atacctcata tcttgatttt gc
//
LOCUS       DAGSWI010000214         4489 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.219, whole genome shotgun sequence.
ACCESSION   DAGSWI010000214 DAGSWI010000000
VERSION     DAGSWI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.219"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            86..2104
                     /locus_tag="MJF80_003698"
     CDS             86..2104
                     /locus_tag="MJF80_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBZ0282302.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIINTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            2319..3176
                     /locus_tag="MJF80_003699"
     CDS             2319..3176
                     /locus_tag="MJF80_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBZ0282303.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            3273..4142
                     /locus_tag="MJF80_003700"
     CDS             3273..4142
                     /locus_tag="MJF80_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBZ0282304.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         1885 a          395 c          734 g         1475 t
ORIGIN      
        1 aaagtcctac cgtaaaaaag cgcattttga ataagataac aaatatgata aaattggtct
       61 tgttgaaatt tttagggggg atatgatgac aaagaaacta gtaatatcaa aaacaaagtt
      121 gtttattttt ataatagcat ttatttctat atttatatat ttatttggaa gtaaaaatac
      181 acttataggt gtaggtatag taacagctat gctaacgctc ttggaaagag atttaactat
      241 ttctccaatt aaaaatttac tgaagtattt agcaataaat ataatcttag gaatactatc
      301 attttttgca gtacaaaata tgtatttagg agttttatta aattttatag cattatttat
      361 aatagggtat gtatttagtt atgacttaaa aagagcagta tatgtaccct ttggtctaat
      421 gtatatattt atggtaagta tacctgtagg aaaaagtgaa tttcctatga gattgtcggc
      481 tttagctgtt ggagcagtta taataatgat tgctcaattt gttatgaata gaaataggat
      541 gaaaaatgtc ggagataaag aactgatatc tatatgtgat gaattattag aaaaaattag
      601 tcttttaaaa aatactatta atgatgatag ttctataatt aaatctagta tgagaaaaat
      661 agatagctgt aattatagaa taaattctat atctaaaaat ttgaaaatgg ttatttttga
      721 taatagaaaa gatgactttt atatcagtat tagaggtata gacataatga atattttatt
      781 ttcattagaa agaataagtt taatcctaga aagatacaaa aaggatagta aagaatttga
      841 agatgaagat ataaaaaata ttttagaaga aagtagcata aatagtaaat cagatgttct
      901 agttgtagca acgaaagaaa taaactatat taagatgtgt ttagaaaata aagataccat
      961 aactgataaa gagatattag gatttagaga ttatgttatt gctcaggata ctaaaaatat
     1021 aaatttaaaa gaaatttata gtgttttaga aaatttatat gagtttttag tagattataa
     1081 aaaagtaaaa tcaagagacg aaaagaaagc tgaaagaaaa tcaaagatac ctcatgaatt
     1141 taaacgacta tcaatatata agaaaaactt taatttaaat tctataagat tttcttatgc
     1201 agtaaagatt gctttagcta cagctgtagc gggttttatt atggattatt tccatttaag
     1261 agatggaaga tggataatgt taactgtctt ttcattaaca caaccttacg cagaaaattg
     1321 tatacaaagg tctagaaaaa gaatagaagg tacatttata ggagctgtaa tatttattgt
     1381 attattttca ataataaaag atagtacatt aaggtcgtta atagttcttt tagcagggta
     1441 tataaactct tatgtagttg attatagaaa attaatggta tgtgtaactg tttcagctct
     1501 tggttcagca gtagttatgg gagacccaaa tgttttaacc ataagcagga tttcatatgt
     1561 agcattagga gcaattattg cattaatagt aaataagttt atattaccat atgatgctaa
     1621 aacaggctat caacatgtta tagaaatgta taaaggaata gttaaaaata taattgatga
     1681 agtcaataag tctatagaaa atgtagctga tgtttactat ataaaaaatt tattattgat
     1741 accatcactt attgaagata gacttatgtt gataaatgct atatataaag ataaacatca
     1801 agaggatttt ttagaaaatc agaaattact aataagtaat atgtataatc tatatataaa
     1861 tgttaataaa aataaaataa aagatgaaga tgttgaaaaa atattgagag atactaatta
     1921 tatttcaaat tacaatgtag ataagtatga tgaaggaaga agtgtaattt tagagagtat
     1981 tataaatact aaaagtctag gtgacaagat aatatgtcta aacttactgc aaactctaaa
     2041 tggtgtcaaa gaaatgtata gaataagtaa tataactaaa gtttcaataa aagaagcagc
     2101 ttagatatta aattatagaa tagccatata tttaaaataa agtatatggc tatttgtttt
     2161 ataaaatact aaaagaaaat aatatataaa aaacaaaaaa catatattta tgaattttat
     2221 atgaaaatag ttaaaatatt gatttttttc attggttgaa aaaacaattt acttaggata
     2281 aactataata gtattcaaat attatggggg ttagatttat gagtaaagat gtaaaggtaa
     2341 agcgtaataa gaaatatgat acatttagta taactatgga acttgagaga cctttacttt
     2401 atgatgaggt tgagaacaaa gatgataaaa ataataatag aaaatttaac ataaagaata
     2461 agatatttaa gggaataggt atactatttg tattgatttt gcttggcttt ttcatatgga
     2521 taaataaaac atatgaacct caaaagctgg caaaagaagc tctagtcagc aatagtaaag
     2581 ttgaagtaac tgtaaatgaa aacatttcat ttacaccaaa aggaagaaaa gtttctaaag
     2641 gtttaatttt gtatccagga gctaaagtag aaatagaatc ttatgctcca cttgcaagaa
     2701 aaatagcaga aagtggatat gaagttgtaa tcgtaaagat gccacttaat ctagctatac
     2761 ttggaactaa taaggcacag aaagttatgg attcttacaa caacatagaa cattgggtaa
     2821 taggaggtca ttcattagga ggagttgcag catcaaattt tgcacgtgat aataaattaa
     2881 ttgaaggcgt tgtattttta gcttcttatc caatgggtga tgagttgaaa gaattaggaa
     2941 aaaaagttat atctatatgg gggagcaaag atggagtagt caattttaaa agtcttgtag
     3001 aatcaaaaca aaaacttcct gatgatacaa cttatgttga gattgaagga ggaaatcatg
     3061 cacaatttgg agattatgga aaacaaaagg gagataatga tgctattata agtcaagaaa
     3121 aacaacttaa tattactgcc aatagtatca ttaaattttt aaagaatata tcttaaacta
     3181 aaatttaaaa aatataattt tataagttat tatttaaagt aaatattaat aatataatag
     3241 aatgaatatt ttattacaag ggggatttta atatggaata cgatatgcca ctttacagac
     3301 caccaagtga ggcttatagt cttataatac aagtcacact aggatgttct cataacaaat
     3361 gtacgttttg tagtatgtac aaatctaaaa aatttaaaat aaagccacta gaagtaataa
     3421 aaaatgaaat tgatatattt agaagatatt ataagcatgt agaaagaata ttcttagcag
     3481 atggagatgc attgataata ccaatggaga aattaagaga tattatttta tatataaaag
     3541 aagtttttcc agagtgtgaa agaattacta tgtacggctc tcctaaatct atagagaaaa
     3601 aatctgatga tgaacttaaa gaattaagaa aacttggtgt taaaatgatt tatctagggc
     3661 ttgaaagtgg aagcaatgaa gtattagaag atattaagaa aggttttagt agtgaacaat
     3721 taataaaagt tggtagaaaa gttaaaaaaa gtgggattaa attatcagcc actgtaatag
     3781 caggtcttgg aggaactaaa aaaacacatc agcatgcagt tgatacagga aaaatgctag
     3841 gagcaatttc accagattat gtgggtgtac ttagtttaat ggtagaacct aatactgaac
     3901 tttacgattt acttaaggca ggagagttta caatactaga ggataaggct gtgttacaag
     3961 aaattaaaga gatgataaag aatatagatg ctaatgaaaa aattgtattt agaagtaatc
     4021 atgcatcaaa ttatgctaat ttaaaaggtg tattacctga tgataaacaa cgtctaattg
     4081 atgaaattga ttactatcta agtaaccaaa agctaaaaag agaagaatat agaagactat
     4141 agtttttgat atatcaatct atactgatag gtaaatacta agattattta gatacaaaaa
     4201 atcatgctac attaatagtt gcttactatt gatgtagcat gattttttat taaaaaacaa
     4261 aatggtatat tcatatataa tatatcaata ataatgaaaa tgtgaactag tttaatagaa
     4321 ttttacctat tataataaaa acaggaattt aaataataaa aagtaaagta actttttatt
     4381 aagtaacttt actttttatt ttatttaatg ttgtattaaa tttatatata ttaaaataat
     4441 atacaaattt tgaaaaagct cttataaaat gattattaaa gtaaagttt
//
LOCUS       DAGSWI010000215         4474 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.220, whole genome shotgun sequence.
ACCESSION   DAGSWI010000215 DAGSWI010000000
VERSION     DAGSWI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.220"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..732)
                     /locus_tag="MJF80_003701"
     CDS             complement(<1..732)
                     /locus_tag="MJF80_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282305.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICI"
     gene            complement(733..1608)
                     /locus_tag="MJF80_003702"
     CDS             complement(733..1608)
                     /locus_tag="MJF80_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282306.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSSDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(1612..1980)
                     /locus_tag="MJF80_003703"
     CDS             complement(1612..1980)
                     /locus_tag="MJF80_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0282307.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(2163..2822)
                     /gene="deoC"
                     /locus_tag="MJF80_003704"
     CDS             complement(2163..2822)
                     /gene="deoC"
                     /locus_tag="MJF80_003704"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBZ0282308.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(2858..4045)
                     /gene="murJ"
                     /locus_tag="MJF80_003705"
     CDS             complement(2858..4045)
                     /gene="murJ"
                     /locus_tag="MJF80_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBZ0282309.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(4122..>4474)
                     /locus_tag="MJF80_003706"
     CDS             complement(4122..>4474)
                     /locus_tag="MJF80_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBZ0282310.1"
                     /translation="TKLIKENDIICIEDLQVKNIIRNRKLSRLISDVSWSEFIRQLEY
                     KANWHGRQIVKVDKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASIN
                     ILKEGLRLITIQNK"
BASE COUNT         1494 a          767 c          437 g         1776 t
ORIGIN      
        1 tatgcaaata actataattc ctacctttgt tccaaataaa ataaaaggat tctgaaatga
       61 ataaataatt ttttctaaat acaatagcat tttatgcata tttttagttg caaaattaac
      121 tatataaatt accattaata tcggtaatcc tatcaatata gtaatactag taacttttct
      181 aactctatat gtaaaagctc caagtaacac tcctataaaa cctatacata tttcatttat
      241 acatatttgt acaaaataat ttaaataagt tagtggcata cttgatgttg aaggatctat
      301 cagtgctgat atagatgtcc aaatcatatc tctctttagt aaaactgata gttctatact
      361 gtaacctgtt ataatttcta taaataactt cgataaaaaa atcaaaacta tacctaaaat
      421 agaaatcaca aagcttaaaa taattaaatc tatgacaata gcttttatac aactttttct
      481 atctgctctt atacttaaag ctcctgaaaa gtctttattt gctatattta ctccatacac
      541 aaatagcagt atagttatat atgttaaaat aggattcaca atcgtggata aattagtttt
      601 agatgcttct ccaaaaatct tgagaataaa cactggcaaa atattcataa caattcccac
      661 aatcataaat ataattatat acaatttagt atttttatta aaaaatttta tatatggctt
      721 taattcattc aattacaatt cctccttctt agtcatgtta acaaacatat cttgtagccc
      781 tatgtatcct acatcaatat ttgaattttt aagtattttt tcatcatttt cactaaaatc
      841 accatagtaa aaataagcaa ctgcatttcc aaatgccttt ttaggtttta tattttttat
      901 acattctaat ttttctaact cttctttatt tccagtaata taatgagctt tttgttttat
      961 gacatcaatt tcttcatcta taataatttt tccctcattt aatattatta catgctccag
     1021 taaatcatct atttcatcaa tcaaatgagt tgatattatt attgtccttg gattttttat
     1081 ataactatct aaaattatgt tataaaactc ttgtctattt actgcatcca aaccaattgt
     1141 tggctcatca aatatggtga tagctgaatt agaacaaata cctattatat ttgaaagtat
     1201 agttttcata cctcttgaca gttgtttata ctttttttta gtatttaaat caaaatattt
     1261 gcataattct ttttctaagc ctttatcata acttttataa aaactagaag aaaattcaaa
     1321 tatctgacct actttaaaat ctgaactata aaattctttt tccctgacaa tacaaacttc
     1381 ttccaaaact tttatatctt cttttgggtt ctttcctaat atttctatct caccttcatt
     1441 ttgaattaac tgatttacca ttatatttaa taatgttgtt tttccagctc cattcttacc
     1501 taataaacca tatatcttat ttttttcaaa gtttagagac atattattaa aaatattttg
     1561 cttcttaaaa ctgtgagata aattttctat cttaatagaa ctactcatat attaatcctc
     1621 cttgaaatta attataatat caaccaattc ttctctattt atttctaatt tgttagcttc
     1681 ttgaagtagg ttttttacaa aattatgttt aaaattttct tttctagctt cttttataat
     1741 agtttttgca ccatctgata caaacatccc tatccccctc ttcttatata aaatattttt
     1801 atcaaccaat atatttatac cttttaatac tgtagctgga ttaattttat aaagctttga
     1861 aagctctgtt gttgatggaa cctgttcttc ttcttttatt ccatttgata gtatctcatc
     1921 ttcaattgct cgtgctatct gaataaatat aggttcttca ttatttaata ctaatttcat
     1981 aggttcctcc tttctccatt agttagttac tcaactaatt aactatataa cttataatat
     2041 cttgaaaaat attttctgtc aatactaaat aaaaaacaga gggaaaattc cctctgtttt
     2101 ttgttcttta tatttataat taaattttaa tttttgtaat cctatcccat aagttaaaaa
     2161 ctttaataat ctgttgtatc ttcatttttt aatattttac atattgagct agttcctaac
     2221 ctttctgctc ctgcctctat aaatttttct gcatcagaaa ttgatttaac tccccctgca
     2281 gcttttattt ttacattttt tccaacatgc tctttcataa gctttatatc ttctaaagta
     2341 gctccacctg ttcccattcc agtagatgtt tttataaaat ctgaacctga cattgttact
     2401 atttcacaca tttttatttt ttcatcttca tctaaataac atgtctctat aatgactttt
     2461 aatactttat ctcctatagc tttttttatt tcttttattt cttttcctat attatcataa
     2521 tctttatttt ttatatctga aatatttaca accatatcaa cttcatctgc tccattttca
     2581 atagcatctt ttgcttcaaa tacttttgta gcagtagttt gatatcccaa agggaaccct
     2641 atcactgtac atatctttat tttacctttt agatattctg atgctctttt cacatatgat
     2701 ggtggtatgc aaactgatgc tacactcata tctacagctt catcacataa aattttaata
     2761 tcttcccatg tagttgtagc ttttaaaata gtatggtcta ctgtttttaa tatatgtttc
     2821 ataaaatcct cctaaattta taattaatat aattagatta cttttttaaa tgcaattttc
     2881 tctttgcaat gtttagtatc atatcaacct cttcaacttt aaaaataatc attagtagtg
     2941 tataaattcc acctccaact ataactgaaa tagctagtga tacaaactca ttaaatgttc
     3001 ctactcccaa aattccaaat ataaatttat acgtaaagta tgataaaaca cccataatta
     3061 aagatgcaac taccgatttc aaagttgctt ttattatttt atcttgtcca aaatatccaa
     3121 cttttctctt taggttcaag aagagtaaca atatacatgc aattgatgat gaactagttg
     3181 caagagcaag accaccatga gccattggtt ttattaaaac taagtctaac actatattta
     3241 cccctacaga tataatccca tttaccatag gtgtttttgt gtcttgtaat gaataaaaaa
     3301 cctttcccaa aatatctctt aatccaaatg cagtcatacc aactgcataa aaaattaagg
     3361 cagttgctgt catttgggtt gccctcgcat caaaagcacc tctctcaaaa atgattctaa
     3421 caacaggagt tgcaaaaaaa atagaagcaa ctgaaatagg aatcatagaa attattatca
     3481 tatttatact agatttaact gaactagtaa acttcttttg attattttct gcacttaact
     3541 tagataacat aggatacata acagatacta ttgaagctgt aaatgtccct gtaacaaaac
     3601 catttaatct atctgcataa gtaagtgctg gaatgcttcc tttaactaat gttgaggcta
     3661 aagttgtatc taccaatgag ttcacttgat taacagcaac acctataaat actggtgaaa
     3721 gtaatgctag taatttaata aggctatcat ctttaaagtt taagtagtat aagtatttgt
     3781 agttcgtttt tttaacaaaa aacatgtaaa agagcagttg aactaccata gcaaccacag
     3841 caccaattgg aagtatgtat ggtccaaata tggtacttaa cattattgaa ataattatta
     3901 ctatattata gggtatactt ccaaatccaa ctacaataaa gttctcttta atttgtaaaa
     3961 atgctgacat aacacttgta atacctatga atattattcc tgttctgaac ctcccatgac
     4021 taaagtcaca gggttcctgc ttcatagaat tttgcatact aaaatatgtc ttactaattc
     4081 tccacaggct atccccgtag ttcctacggt tcttacataa gttatttatt ttgaatcgtt
     4141 attagtctta atccttcttt taatatattt atacttgcat ttatatctct atcgtgagtt
     4201 tcattacaac taggacaaat ccattctctt atatttaagt tcttaacttc ctcgttttta
     4261 tacccacatt tattacatat ttgtgaactt gcaaagaatt tatctacctt tacaatttgt
     4321 ctaccatgcc aattagcctt gtattctaat tgacgaataa actctgacca tgatacatca
     4381 gaaattaagc gagatagttt acgatttcta ataatattct ttacttgtaa atcttctatg
     4441 caaataatat cattctcttt tattaattta gtag
//
LOCUS       DAGSWI010000216         4413 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.221, whole genome shotgun sequence.
ACCESSION   DAGSWI010000216 DAGSWI010000000
VERSION     DAGSWI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.221"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..514
                     /locus_tag="MJF80_003707"
     CDS             <1..514
                     /locus_tag="MJF80_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBZ0282311.1"
                     /translation="FLLYSMVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYL
                     AGSYLLKKYSSLNLLKLSAIMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKR
                     LIFEVSPKKLRSSSQLIALSIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAY
                     GLIVLLNYLQ"
     gene            834..1586
                     /locus_tag="MJF80_003708"
     CDS             834..1586
                     /locus_tag="MJF80_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282312.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            1587..3494
                     /locus_tag="MJF80_003709"
     CDS             1587..3494
                     /locus_tag="MJF80_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBZ0282313.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NMLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVMIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIISITVIGYGYY
                     LSTNIRTGNITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYDERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            3646..4327
                     /locus_tag="MJF80_003710"
                     /pseudo
     CDS             3646..4327
                     /locus_tag="MJF80_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT         1776 a          415 c          719 g         1503 t
ORIGIN      
        1 attcttatta tactcaatgg tagtagcaaa taactgtact gtaatagata aaatgatagc
       61 tttaaatgct acaaattctc agatatcttt aaagtggtct ttacagtcat tattagagat
      121 accaacatat ctggctggtt cttatttatt aaaaaaatat agcagtttaa acttattaaa
      181 gttaagtgct attatgataa cagttcagtt tctattattt gcattaacaa atgattcaaa
      241 tatcattatt atactgtgtg tttttcaagt gttttctact cctttaattt taattacttc
      301 aaaaagatta atatttgaag tttctcctaa aaaacttaga agtagtagcc aattaatagc
      361 tttaagtata tttatgggag gttcatctct gattattcca actgttgctg gttttttgag
      421 cattaatatt gggtataatt atacattaat ggtattaagt ctttttggat gcttagctta
      481 tggcttgata gtcttattga attatcttca gtaataattg ttttaatata tagcagtaat
      541 atcacttgtt tatagtacct gtatttgcca caatcagggt tgtattttgg ttttttatag
      601 agaagaataa ttgtatagat tagaggcttc ttaggagctt cttttctttg tttatacaaa
      661 gaagtaaatc atgcttttat tttaagatat ttctactata attataatag tcttccatat
      721 gttacaaaag tgtaatgcta acagttaaaa tgtaatattt acttaaggat ttatttatat
      781 aaaaccttta aaataaatat atattaaaaa attagcaact ggaggctatt gttatgaaag
      841 aaatattaaa aataaaaaat atatcaaaag attatggaat aaaaggattt aaaacaaatg
      901 tattaaaaaa tatatctctt acagtcaatg aaggtgattt tatagcaata atgggtccat
      961 ctggtgcagg aaagacaaca ttgctaaatt taatgtctac acttgacaaa caaacttcag
     1021 gagaaataat actagatgga attaatatat caaaagtaaa aaataatgaa ctatcaaaat
     1081 taagaagaga aaaaataggg tttatatttc aagactataa tctattagac aatatgaaac
     1141 taatggacaa tatagcactt cctttagcat taggaaaaaa gaaaagcaag gagatagaag
     1201 ctaaggtatt ttctatagct aaaaaatttg gattagaaaa tcatttagat aaatatccat
     1261 atcagttatc aggaggacaa aaacaaagag gcgcagcagc taggtcatta attacaaatc
     1321 caacagtcat atttgctgat gagccaactg gtgctttaga ctctaaatca gcatatgaac
     1381 ttttagaatc attagaaaaa ataaacagag aaaataacgc tactattata atgattacac
     1441 atgaccctct aacagcaagt tattcaaatg aagtatatat gataaatgat ggaaatataa
     1501 agtgtaaatt aaacaaagga aatagtagaa aagaatttta tggaaaaatt atggatatgc
     1561 tagcctctat gggaggtgag atgtaagtga gtatacttgg aattgcttat aataatttta
     1621 agagtaacat aagaacttat ctagcatttt ttatatctat ggttttttca gtagttgtgc
     1681 taactaattt tgaattatta agatatggtg atgctataaa tatgttacaa gaggaaaata
     1741 aaaaatttac actatcagtg ttagtagctg taataatgat actatcagta tttttatttt
     1801 tctttatatg gtatgcaaca aatatatttt ttaagaatag gtctaaagaa attggaatat
     1861 tgtcttttat gggattagat ttatatacta taggaaaaat atattttgta gaaaatatgc
     1921 ttataggaat aagctcttgt ataacaggaa taattatagg gataataaca tctagatttt
     1981 ttcaagtaat gataataaag ttatctggat ttaatataaa ggtatctaat ggacttagca
     2041 tagaagctat actgtatgct actttaatat ttatttctat atttataata atggctgtaa
     2101 aaggttttat aacaatatgt agaagtagtg taataaatct aatcaatata tctaaaaaac
     2161 aagaaaaaat accgaaggta ggaataggta tatatatatt agctataata tcaattacag
     2221 taataggata tggctactat ctttctacca atataagaac tggaaatata actagtgtac
     2281 tacctgttat aggtataata atagtaggta cttatggctt attcaaatca gttatgcctg
     2341 ttatatttga tattataatc aaaaataaaa aaatattatt taatggaaat aatataattg
     2401 caataaacaa tataaactac cgattaaata aaaactataa aacttatgct ataatagcta
     2461 taattataac tacaacaata tcaactcttg gtgcttcagt tgcgatgaag cgtatacata
     2521 agaatgccca agaacaaaga aatgtgtaca ctattagcat agtttcaatg gataaagatg
     2581 aagtgaataa aaaacaaata gagaatataa taaaagatac taataagata aagtactcag
     2641 taaatccaga actaacaatt ttaaagaaaa cagatataaa agaagcttat gatacaacag
     2701 atactataat gaaatatagt gatttttgta atactcttaa agtgaatggc aatgataaag
     2761 aattaaaaga gtatgatgaa agattagtta gtggaaataa tgtaatagag ttaaaaagcc
     2821 ctcaaacgat agcatcaata gctacaaaag aagccaagat aaaacttgat ggtcagttat
     2881 ataatatttc aaggggagaa gtaaaagttc cagtactagg gacaggactt aatcaaagta
     2941 ttatagtggt aaatgatgat acatatttta aactaagaga taagggtcaa caagtgtatt
     3001 tttatggtgc aaaggtattt caagaagaaa atagtcaagt aatgttcaac aatattgaaa
     3061 agaatttaag tttaaaaaat gggtatattc aaaatggata taaagctcaa ggtgagagtc
     3121 aatggatgaa gtttgcatat gtagtgcttg tgtttttatt cttagtcttt gcaattgtag
     3181 ctggaagtat aatatatatg aaaatatata gtgatgcata tgaagataaa gagaaatata
     3241 agattttgtt aaaaataggt actacagaaa aagatataaa tagagctata cttaaagaag
     3301 tagctatttt ttatactttg ccaatgttaa gtgcaactat atctagttat tttgcattga
     3361 gattagcagg agatttattg atgaccgatt tatttggtat atatatacta agtttagtga
     3421 tatgtttgat aatattcata atatatggag cagtatctgt taataagttt aagagtgtag
     3481 tgtataaaaa ttagttgata tattaataaa gagaaaggag attcttgtaa taagagtctc
     3541 cttgttgatt aactaaataa tttgggtaat aaattcatat aaaatatgtt atgatattaa
     3601 taattcatag catagtgaaa tattaggata atggagaata tacaaatgta tagaatttta
     3661 ttagtagagg acgatattga tttatcaaaa gagattgcat tagcacttga aaaatgggga
     3721 tttaaagtag aattaattga tgattttgag gtagtattag atgaatttat agataaaaaa
     3781 ccagatgtag tacttttaga tgtaaattta ccattatata atggatttta ctggtgcgaa
     3841 aaaataagag ctatttcaaa tgtaccatta atatttttat catcaagaga ttctgatatg
     3901 gatttaataa tgggtataaa taatggagca gatgattaca ttaccaaacc attttctata
     3961 gaaatattgg ttaccaaaat aaatggtata ataagaagag tctataatta tagtgattct
     4021 aatagtattt tgtattgtga agatttgatg tttgatgtgg ggaaagggat tataaaaaca
     4081 taagtataag gataaaagta tagagttgac taaaaacgaa ataaaaattt taacattatt
     4141 attaaaaaat aagaataggg ttgtatcaag ggaaagtctt atgatgactc tatgggataa
     4201 tgatgagttt gtgacagaca atgcacttac tgtaaatatg aatagattga gaagtaaagt
     4261 taaagaacta ggttttgatg attttataaa aactaagaag gggataggat atataataca
     4321 atgctagtaa aatatttaaa aggtaggtct aagtatattg ctctaccttt agttgtagtt
     4381 agtataataa atatctactt atttgctata gat
//
LOCUS       DAGSWI010000217         4391 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.222, whole genome shotgun sequence.
ACCESSION   DAGSWI010000217 DAGSWI010000000
VERSION     DAGSWI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.222"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(213..1256)
                     /locus_tag="MJF80_003711"
     CDS             complement(213..1256)
                     /locus_tag="MJF80_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBZ0282314.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
     gene            complement(1277..1723)
                     /locus_tag="MJF80_003712"
     CDS             complement(1277..1723)
                     /locus_tag="MJF80_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBZ0282315.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            complement(1897..3477)
                     /locus_tag="MJF80_003713"
     CDS             complement(1897..3477)
                     /locus_tag="MJF80_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBZ0282316.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRLSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSTLIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT         1419 a          766 c          426 g         1780 t
ORIGIN      
        1 tatataatat tatataacac tttttacatt agtaaaggat aaattgtata aagattctgt
       61 taatttattt ttataatttc atctttctaa taaaaacaaa gaagaagact taaaaattta
      121 taagtcctct tcttttcaac gtgcattttt gtgctatatt taattaattc gacctttaat
      181 ttccatatcc ttcctatgat aacaaaactc ttttattttt ttaatgattc attatcattt
      241 aaaacttctt ttatatcaag tacttctttg ttatcatcac tcataacttc ctttattcct
      301 ttgaatgttc ctaggaaatt aactgcttct gaaggtaaaa ccagttttgt tgacttacct
      361 tcagccattt tttcaagagc ttccatagat tttaatgcta gaatattatt gtctatatca
      421 gcctctttca ttgctgaaaa aactaatttt aaaccttctg ctgttgccat ttgagttctt
      481 tttatcattt cagcttcacc ttgtgcctta gcaatagctg tctgtcttat agcttcagct
      541 tcaccttctg ccactaatat agcagattct ttttcacctt ctgctacacg taccatagct
      601 tcttttttgg cttctgctgt taatattgca gattgttttt caccttcagc ttgaagtatt
      661 gcagctgact tattaccttc tgcttgaagt attgcctctc ttctttctct ttctgctctc
      721 atttgttttt ccattgcaac ttgaatatcc tgaggaggca ttatattttt taactctact
      781 ctatttactt ttatacccca tttatctgtt gcttcatcaa ggatagttct catttttaca
      841 tttattatat ctcttgatgt taatgtttca tctaaatcaa gttcaccaat tatatttctt
      901 agtgttgtag ctgttaaatt ttcaatagca gctattggat tagctatctc aaacacatat
      961 ctaactgggt ctgtaacttt ataatataca actgtatcaa tctgcatagt tacattatct
     1021 ttagttataa ctggttgagg tggaaaatct attacaattt ctctaaggtc aattacatat
     1081 gccattttat ctaaaaatgg tattaagaaa tgtacacctg tttcagccac tttttgaaac
     1141 ttaccaagtc tcattattat acctacttta gattgtttta taactcttat acaagttaaa
     1201 cttattgcta cgactactac tattaataca atacttaaaa caattttagt acccatacta
     1261 taaatcctcc tttattctat tggtttgtca cattaactgt ggcgtctact tttttaacaa
     1321 ctaattttac cccttcaatt tttaatactt ctactgtaga acctttttct atcttttcac
     1381 catctataga aatagccgtc catgtttcat gttcaactac aactagacct gtgttatttg
     1441 gagaaatctc ttctgtaaca ataccttttt tagacatcat tgcttgtaaa ttagtgttag
     1501 atttatagcc tttatctttt tttactattt ttttagtagc tacgactaac attgctatag
     1561 aaattactgc aaatattaaa atttgtaaga aaatactctc tataaagcta cttaaaaata
     1621 taagtattac agctcctaca ctaaaccata tcaatgttaa gcttggtgtt aacatttctg
     1681 ctattccaaa taagactgct actatcaacc atattaactt catactatct accctctttt
     1741 catatgaatt ataaaaccct atataaatta tatgtttata caatgttttt gaatataaat
     1801 ttaaattaca aaacaataaa ataaattaat taattaatag tattatatca acatactttg
     1861 ttaaaattgt tacaaagtag tatcaaatat caccaattat atatccaaca actctttcat
     1921 tgttaatgaa atgttactta ttaaatccgt agcatttacc gtatacattg ctttgctcaa
     1981 tttatctcct atatagccat gtatatatgc tccactaact gtagcttcca atatatccat
     2041 accttgacct ataaatgaag taatcattcc aagcaaacaa tcacccattc caccagttgc
     2101 catacagcta tttcctgtag gatttatata tgttttttta ccatctgtta tgacagtttg
     2161 ataacctttt aaaagcacca ccagattata tttttgagca aattcttttg caacatctac
     2221 tctatgttct cttatatacc caattgaatc tcctgtcaac ctggccattt ctcccaaatg
     2281 aggtgtaatt acaaatctat ttttccattc taaaaactta taacatctgt ccttcataac
     2341 gttcaatcca tctgcatcta atactattgg gcaattagaa ttttctacta tcttaagtaa
     2401 tttatcaaat gtttgactat tatctcccat acctggacca aaaccaattg catcactaga
     2461 attaagtaat ttatttaatc tttcttcttc aaaatttaaa gtcattgctt ctgtaagtct
     2521 aatagatacc ttttcttgca catcttcact actaattaat gttacaagtc cactcccact
     2581 cttaacagca gattcagttg ctatgaaact tgcaccataa aatccttttg aacctgcaac
     2641 tatagatact tttccaaaat cacttttaaa accatgctta tctcttcttc taatattatt
     2701 ttttacaaaa gtcttatcag ttatgaactc ttcatcatgg tattttcttg taataaaatc
     2761 tggaacacct atcttttcta caactacatc acctataagt gtgctagtgt catagtttag
     2821 aaaacctctt ttataaaact caaagctaat cgttttgttt gctctaatac aagttcctaa
     2881 tacatttcca ttatcgctat ctatacctga aggaatatct atagaatatg tatttttgct
     2941 attatcgttt actatgtcaa taacttcttt aaaaatacca gttatatcac gttttaatcc
     3001 agtcccaaat atagcatcta ctacaacatc acttttagat agtctatctt taaaaattgt
     3061 taaattttta ttttttataa tattttgttt ctcaacttta ataattttat ctacttcatt
     3121 ttcatctcca taattttttt cacttaaatc atttttatta atatttataa tattaattcc
     3181 catatttaaa agtacattgt aatttatttt agagcattca cttagttttt caagattacc
     3241 tactaaaaat acgtttactt cttttccata tgtatttaga tgtcttgcta ttccaagacc
     3301 atctcctcca ttatttccaa caccacatac aactgtataa ctattataca tgtctatatc
     3361 catatttttc attgcagata taaccgcatt ttccatcaat acaatcattg gaatccctac
     3421 ttcttctaca catgcagtat caacatttct gcttaattta gatgtaccta ttagcataaa
     3481 ttatcactcc tataaagtta aagacttctt aaatacttac caaaaatatc tattgaaatc
     3541 tttttaatcc cataaatatc ataaataaaa tcactaaaac taagagtttt gaaataattt
     3601 aaaataacaa aaaattttga attaaaacaa ttatttaatt tattatacca tataatttaa
     3661 gttattatgt tgatttaaaa cctatttaca ctaattatct aaatatctct ttatttttat
     3721 aatcttttat gacttattta tatttttcat ttttataaaa aaacaataat taagaatata
     3781 actatattct acatgtaaaa atttttttat ttaaactcaa atttctgata atttgataat
     3841 ttttcaaaat ttatagttga cttttatttt ttactgtatt aaactaatta ctatacaaaa
     3901 cagaaaacaa aattttcaaa attattaaat taaaaggaga ggtaaatatg aagactttat
     3961 taaataaaca agtacgattt tttagtagcc aatttagtta gggtttgtac ctaagttaag
     4021 acaaattgtc ataacataga tacaagcccc ttaggattat gatttttaag gattgtatct
     4081 atgttggcta tagtaattgt tgttaaagtt tttattttat catcataata tagagccaca
     4141 tggattatac tgtgtggctt attcgaattt ttgccataca gttatatata ctgtgtggtt
     4201 tttttatatc aaaatcattt tttaaaacca aatttaaaaa accatttaaa atattttcaa
     4261 aggggtgttt tatttatgtc aggtattata tctgtaatga cgcaaagttt aattttatcg
     4321 attatggcac tgggggttta tataacttac aaaatcttag attttccaga tatgtctgct
     4381 gatggtagct a
//
LOCUS       DAGSWI010000218         4359 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.223, whole genome shotgun sequence.
ACCESSION   DAGSWI010000218 DAGSWI010000000
VERSION     DAGSWI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.223"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(100..645)
                     /locus_tag="MJF80_003714"
     CDS             complement(100..645)
                     /locus_tag="MJF80_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBZ0282317.1"
                     /translation="MKKFVCTVCGYIHEGDTAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(763..2586)
                     /locus_tag="MJF80_003715"
     CDS             complement(763..2586)
                     /locus_tag="MJF80_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282318.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDINTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMVSNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTILSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(2599..>4359)
                     /locus_tag="MJF80_003716"
     CDS             complement(2599..>4359)
                     /locus_tag="MJF80_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282319.1"
                     /translation="KYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGVSNKNL
                     DYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLKQIDKF
                     GRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIILIAVLI
                     IFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSFEELND
                     ELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYVNYMTR
                     ILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFVNCDII
                     FENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYIIDKGDI
                     KIGGRSIYSISEESLRERIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSCEIANA
                     NQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMDINTET
                     IIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATHEDLLV
                     SSPIYKKIFALQNLVSEDVN"
BASE COUNT         1419 a          780 c          390 g         1770 t
ORIGIN      
        1 ttagaaataa aaaataaagt acaaataatt aaaaaatatc tcactcataa ctttaaaaat
       61 tataagtgag atactcctaa aatctacttt atattaaatt tattttccaa aatatctatt
      121 taataatcct aagaatgctt taccatgtct agcttcatct ttacacattt catgtactgt
      181 atcatgtata gcatctaatc ctaattcttt tgctttctta gctaatttta attttccatc
      241 agttgcacca tactcagcat caactctaac tcttaagttt tcttttgtat ctgcaactac
      301 aacttctcca agaagttctg caaatttagc agcatgttca gcttcttcaa gagctattct
      361 cttataagct tctcctactt ctggataacc ttctctatca gcttgtctac tcattgctaa
      421 atacattcct acttctgtac actcaccaac aaagttagct cttaatcctt cgattatttc
      481 ttcatctaca ccttgagcta ctcctattct gtgctcatca gcccaatttt tttcgccttt
      541 catttcttca aatttatcag ctccaacttt acatactgga cattgtgctg gtgcagtatc
      601 tccttcatgt atatatccac atactgtaca aacaaatttc ttcataatta ctttcctcct
      661 aaattttata tatcaaatta aattttttat ttattaagtt agtctatata tacccgcacc
      721 atataccttt aaacaatttt tttaaaagtt ttttctaaat acctattttc cctgtgtcaa
      781 taatatgttt ctataataat gtccctttaa ttttatcaat tctttatgat ttcctgattc
      841 cacaattcta ccattttcta aaactattat attatcacaa tttgatatgg tacttaatct
      901 atgagctata attatacttg tctttccata agttaaatta tttattgctt cttgaatttt
      961 agcttctgtt gctatatcta tgttacttgt agcctcatcc aaaataagta ttggagcatt
     1021 ttttaatact gctctagcaa tacttattaa ttgtttttga ccttcactta aatcttcacc
     1081 cctatccgat aatatagtac tatatccttc acgcatttta actataaatt tatgtgcatt
     1141 tgctatttta ctagcatata taacttcttc atcagtagcc tccagatttc cataccttat
     1201 attttccata actgtatcat tgaatatata accttcttgt ggaactactc caaaacaagt
     1261 tctcaaatag tttctgttaa tagagtatat atccttacca tctaaaaata tacttccttc
     1321 atctatatga taaaaatttg taattagatt tataatagtt gtttttccac ttcccgtctc
     1381 tcctatgatg gcatttgatg tgccagcttt gatttttagg tttatattat ctaaaatttt
     1441 cttttttgaa tcataagaaa aacttacatc tttaagctca atttctccct ttacaaaatg
     1501 agagtttaaa ttaccatctt tattatcgtt aatctcactt ttaaacatgt tatgcacttc
     1561 ttgctcttct tgttcatcta taatttcaaa aactctttca catccagcta tggctgaaag
     1621 taatgtatta aatagatttg ctaattcatt taaaggtctt gtaaattgtc ttacataact
     1681 taaaaaacta gctataaccc ctatggttat aatatcttta gatgcaagaa tgcctcctat
     1741 aatacatata gttgcaaatc ctatattact aatcacattc ataataggca ttaaaagaga
     1801 agtgtaaatt tgcgatttaa gggaaatatt tagaagtcta tcattgactt ctttaaaatc
     1861 atttacagat tttctttcat aattaaaaga ttttactaca tttatgtttg ataccatttc
     1921 ttcaatatgt gcatttagtt ttcccaactc aacttgttgt tgcttaaaaa agactctagt
     1981 tctctttgca ataaaattac tcacaaacat tactatcggt attaatatta cactgcaaaa
     2041 ggttaaaatt gggcttaata caaacatcat aataaaagtc atcaatatgt ttaagattcc
     2101 tgtaattaac tgtactaatg aatttgatat tcctgtactg atattatcaa catcatttgt
     2161 cactctactc ataatatctc cagtagaact tttatcaaaa aaggttattg gtagattttt
     2221 aaattttaaa aataaattac ttctcatttc tctgactata ctttgagata atctagctgt
     2281 acatatcccc ttagacagtt taactgaaaa ctcaattata taaaatgaaa gtaaaataat
     2341 tattcctttt aagaaaatgg attttgaata agtattgata tctattatgt caactacttt
     2401 tccaatagta tatggtatta cagttgatat aattgaatca attattacta gcaaaaaaat
     2461 aaatctagta atcttcttat cttttttaaa gtacataaaa attcttttaa acgttttttt
     2521 aaaatcttta ggcttttctt ttttctttaa tctactgacc ttctgatttc cagtacttaa
     2581 attcaatata cttcctcctt aatttacatc ttcacttact aaattctgca acgcaaaaat
     2641 ttttttataa attggagaac taaccagtaa atcttcatgt gtagcaaatc caaccacctc
     2701 accctcatcc aagactaaaa tcttatcaca gtcaattata gaactaatct tttgtgaaat
     2761 agttattaca gtacatttct tactttcctt ttctcttaaa ttacttatat actcttttat
     2821 attctttctt ataattgtct ctgtattaat atccattgca ctaaaacaat catcaagaat
     2881 taatatttgt ggttttttgg ctatagctct agctatagta attctttgtc tctgtcctcc
     2941 tgataaattt ttaccccttt ggaataaaac ttcatcataa gtattttcga gttttgttat
     3001 aaattgattt gcatttgcta tttcacatga ttttacaaca tcctctttgc atatatctct
     3061 atttcccata actacattct ccaatatact ttttccaaac acactactat tttgaaatac
     3121 tacacctatt ctttccctta aactttcttc agaaatactg tatatactac gtcctccaat
     3181 cttaatatca cctttatcaa ttatatataa tttttgaatt aaatttacta aagaagtctt
     3241 tcctgaacca gtttgaccaa tcacaccaat attttgacct tcttctattt taaaggatac
     3301 gttttttaat attttttcat ctgtattttt gttatacgaa aaatctacat tttcaaaaat
     3361 tatatcacag tttacaaact ttaaatcttt ttctccaacg tctaaacttt cttctctctc
     3421 aattacttct tcattctcat caaccaaaac ctcacttatt ctctcataag atgcttttgc
     3481 cctaacaaaa acattaaaaa catgtgaaat cattataaga gatgttaata ttctagtcat
     3541 atagtttaca taagcaacta taatcccaat tttaatttct cctgaataaa ttaaatgcct
     3601 tccaattagt aatattatta ttatggaaac attaatgatt attgtaattg tcggtctaaa
     3661 aaatgacatc atagttgtag cttttctact atttttaaaa agttcatcat ttaactcttc
     3721 aaagctttgg ttctcataat cgtatctaca aaatagttta actactttta ttccacttaa
     3781 aaactgtctt aattttgaat ttactttatc tgtactttct tgtacatttt taaataatct
     3841 ataccctatc ttcatattaa gcaatattat aataaatata attaaaactg caattagaat
     3901 aattattgac atctttaaat ttaattttat agctataatt aaacttccta tacacattat
     3961 tggtgacttt ataaaaactc tcatcattcc attaacgaat tgttgcattt gattgatatc
     4021 attagtaatt cttgttatta atgaagctct accaaattta tctatttgtt ttaatgagta
     4081 tgtatttatc ttttcaaata catcctgtct taaatcataa gaaaaatgct gagaaactct
     4141 tgccgagatt acacttctta ttattgcaaa tattgcacct aaaaaagtta tacctatcat
     4201 tatagctcct gttgatagta tgtagtctaa gttcttattt gaaaccccta tatctattat
     4261 ttttgccatt atagttggtt gagctaaatc acaaaaagct tccaatgtta aaaaaaatac
     4321 tgctgttaga aataatacta tatatttttt tatgtattt
//
LOCUS       DAGSWI010000219         4344 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.224, whole genome shotgun sequence.
ACCESSION   DAGSWI010000219 DAGSWI010000000
VERSION     DAGSWI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.224"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            472..951
                     /locus_tag="MJF80_003717"
     CDS             472..951
                     /locus_tag="MJF80_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBZ0282320.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAINNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            948..1727
                     /locus_tag="MJF80_003718"
     CDS             948..1727
                     /locus_tag="MJF80_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282321.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANDVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1977..2477)
                     /locus_tag="MJF80_003719"
     CDS             complement(1977..2477)
                     /locus_tag="MJF80_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0282322.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(3089..3796)
                     /locus_tag="MJF80_003720"
     CDS             complement(3089..3796)
                     /locus_tag="MJF80_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBZ0282323.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
BASE COUNT         1591 a          641 c          465 g         1647 t
ORIGIN      
        1 ttatgttcat ctctatctaa aataatgaca tttctaactc ttaattctca tttaagtttc
       61 attgtttttt cttaaaagcc tacttttagt caagatactg ttatctttac tcattattta
      121 aaaaacttat tttaaatctg tacatgaagt aaataatcca aaagcattgt tattctaact
      181 actcaacatt aaatacttac tattttctaa tttactcaat ataaacactt actataaaac
      241 taaatatata aatggcttct aaagtttaat atatctacat ggctttatat atgaggggaa
      301 aatactagaa aaaaatatat tatagaatat ttgtatagaa taggcaaaac tttcaaactt
      361 aaatttatca aataatcata aaaaaagtga accatctata gtaaaatttt atctaataag
      421 tgaaaggcaa aaaagtagcg ctacgatttg catagaaggg aaggaactta gttggataaa
      481 actacattta caaacaatat acttgaatca gaacagactt tgtatcgagt atctaagtct
      541 attctaggaa acgaccaaga ctgtgaagat gccatcaaca atgctatttt aaaagcatat
      601 gaaaagttgg attctcttaa ggaagaacaa tactttaaaa catggctgat tagaatagtc
      661 ataaatgaat gtaattcatt aagacgcaaa cgattaaaat ctctatcatt tgaagatgtc
      721 ttcaaaaaca aaaaaattga tgaaaaagat gactatagcg atttgtatac tgctattcaa
      781 agtctaccta aaaaaattaa aattccaata gtcctttact atattgaagg ttattcagta
      841 gatgaagtta aggaaatatt agatatacct caaggaactg taaaaagtag attatcaaga
      901 ggaagaagat tattaaaaac taaattagaa aacacggagg taatcatatg aataaaaata
      961 tattaaataa ttcgtttcca gaagttccag aaagttttca taatagttta agcaaaacac
     1021 taaatagttt accagaaaga gaggaaaatt ataaaatgat taataataaa acacacaaat
     1081 taccatttag aaaaggctta gttgcaactt tagccattac tttgatattg agcacaacag
     1141 cccttgcagt aggacatata tatagtttag ttggaaaatc aagtactaag gctacctata
     1201 catctatccc aactacagaa caagtaaaaa aagactttaa ctttacccct aatatagttt
     1261 ctgaatttag taatggatat aaatttaaag gagcatatac tgtaaataaa aaagcatttg
     1321 acaaagaaaa taatgtttta ggaaaatcaa aagcacttag ttttgaatat aaaaatggca
     1381 atgatgaaat atctcttgac acagcaaatg atgtacttgg gaagagttct gacaatctaa
     1441 aagttgtaga tacttacaaa gatattaata tttcttatga agcattcgca caaaagtttg
     1501 taccagaagg ttataaaatg acagaacagg ataaaattga tgaaaaatca ggcaaataca
     1561 catttagtgt ggggccaaat aatgaatcag acaaagttga agttaataaa tttaagtttt
     1621 tgacatggaa acaagatgga gtttactatt cttttacagt tcaagactca aatttatcta
     1681 tgaatgactt agttaaaatg gctcatgaag taatagatgc aaaataaaca taagtaataa
     1741 taagttgttt atgagtttat tatttatgac tttaattttt ttattagtaa acatggttgt
     1801 atcttccatc tttatatttt ctttaaattt aatatataaa attttagata tttagctacc
     1861 ttcaaaagaa ccattattaa aaagccctta aaaatatttc ttttatatgg aattttaagg
     1921 gctttaactt tattttatac aagtgtatat ggaattttaa agactttaac tttattttat
     1981 ataagtgtaa gctccatttg aattacttct tcttttacag gtctatcatt ccatgtaaaa
     2041 ccactagata ttgtatttac ctctacaaac cctaaagatt tataaacatg aatagctcta
     2101 ctattacttt taaatacatc aagctcaatt ttttcatacc catattcttt tgataaatca
     2161 ataatttgtt ttaaaagact acttccgata cctttttccc tatactcttt aagaataccc
     2221 atgcctagat aaccaacttt ttcagtttta ggtaaaacgt cacaccaacc aatacagtta
     2281 tctgattcta aatcaattat aaacagttgt ggtaagtttt tatttataat attttttata
     2341 aattcaactg tttcttcaaa aggaaaggct tcatccatag ctagatattt cctctctttt
     2401 gctattttat caaaagtttg ccaatacgaa taaatatact tttcttctgc ctttataaat
     2461 tttatgcctt ctatcaactt aacatttccc ctttttaatt tttgattgta gttaacttat
     2521 tttatatgat tctattttat aatgctatat aaattactat atattagctt aaaatatctt
     2581 tgtatatttc tataaaatta aaaccaatat atctatctct tgtatctcta ttaaaataat
     2641 ttttaatagt ataataaata tagctcttta atcttttgag tatatatact taatcatttt
     2701 gaaatttgat ttaaagtata ataaaatata taaaaaaata tataaaatgg tactataaaa
     2761 tttttagtct tcatcaatac cttttttgct ttcattgtta aagctatttt ttctattagt
     2821 ttatttctta taaaacaaac atttagtatt aaaatatata tcacacaata gtatttgcaa
     2881 tttaacttta aaaaaataaa aatacccaac tgacctaagc aactcgattg ggtataaata
     2941 atctaaaaaa tcatttttgt ataaactctt tatttaactt acatttttat ttaccttcta
     3001 ctttaattca acttatattt ctatgcttct tatactctca ttcaacttat atttctactt
     3061 atcttatact tttataaatt catcacaatt atttagttct cgctatcaaa gaaattgtat
     3121 taaaatactt tccattctct acttccatca tttttttgtc atttatagca tatgaattat
     3181 cacaatttat ccaatccttc caagcttcat catgacaatt taaagcttca catttctcta
     3241 caatcacaga ctccgattca gaccaaagtt tattccacca tgtaattgaa tggaagttca
     3301 tattatcttg ccaaaatgga atgagttcat ctggtataca atccacgaaa tcttctttta
     3361 atccaggcat tgccatagca agtattcctc cctcttttac aagaggtgat atatgattgt
     3421 ctaaaaatcc ttctttgttt ccaaagtagt gataagaatc gatacttatc accgcatcaa
     3481 agaaaccttc tgcataaggt agttcatgtg cctctgcctg tattggaaat attttatcat
     3541 ccaatttaaa ctctttaaat cttttatagt tttcagttgg ttctatccat aaatctgttg
     3601 caaatacagt agcatcaaat tcctttgcta aaaatatcga agatataccc ttaccacaac
     3661 ctaagtctaa aatacgcatt cccttttcta gttttatctt acttgttaat tcttccaata
     3721 tttttataca attaggtccc atcatatttt tcatgagaaa gtccttatcg tatttattac
     3781 tttttgtaaa tatcattcta tctttccttt ccctatcagt tattatttat taaattttat
     3841 acgtttgatt ttttattttt tatatgcttc ttatattgag caatctcttt tttattcatt
     3901 ctttaacaga caattttaat gtgtaatttt atccactctg tgatagtaac tttaatgaat
     3961 aatcttatct actgtgatag ttaactttga tgggtaattt tatctactcc acagcaatcg
     4021 ctttaatgag taatcttatc cactctatga tagttatttt agtgagtaat tttatctact
     4081 ctacagtaat cgctttaatg agtaatctta tccactctat aatagtcact ttaatgagta
     4141 atcataattt ttatatcatt ttagatactt actctaataa gaagtcattt tctaatactt
     4201 aataagtttt aattacttcc actttaacct atatactaat taacttattt aatttagtgc
     4261 atatttgaca ctttaccttt tattatacca tttattatca taaatataat aattctattt
     4321 ttttaaaata aatatttata ataa
//
LOCUS       DAGSWI010000220         4198 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.225, whole genome shotgun sequence.
ACCESSION   DAGSWI010000220 DAGSWI010000000
VERSION     DAGSWI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.225"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            431..1339
                     /gene="trxB"
                     /locus_tag="MJF80_003721"
     CDS             431..1339
                     /gene="trxB"
                     /locus_tag="MJF80_003721"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBZ0282324.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1429..3252)
                     /gene="typA"
                     /locus_tag="MJF80_003722"
     CDS             complement(1429..3252)
                     /gene="typA"
                     /locus_tag="MJF80_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBZ0282325.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3464..3706
                     /locus_tag="MJF80_003723"
     CDS             3464..3706
                     /locus_tag="MJF80_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282326.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3854..4045
                     /locus_tag="MJF80_003724"
     CDS             3854..4045
                     /locus_tag="MJF80_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBZ0282327.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1537 a          543 c          669 g         1449 t
ORIGIN      
        1 atagttaata atgtttaatt tgaagaaatt gttagtgtga aattacatat atattcaaat
       61 taaattcata aatatttcac ataataatag tatactgtaa acaaaaggag gtattaaata
      121 tgggaatagt acttataaca gcagcattag ttttggttat gtatggactt tcaattctat
      181 catatatatc agaaagcaat atattagaag acactgtaaa tgataaccaa taaggttttg
      241 aattatataa ataagtgaga actgtaaata aaagttaatt tttatttgca gtttttttat
      301 atatatgtaa aataaagtta attaataaaa aataatgttt tgtttatagt atatatggat
      361 aataatagta aagtataaga atgatgagaa taatatataa gaaataataa tataaagaga
      421 ggtaaacgtt atggtagata tcattgtaat tggtgctgga ccagcaggtt taacttcagc
      481 aatttatgct atgagagcag ggttaagcgt tacagtattt gaaaaaaata tttatggagg
      541 tcaagttgca agtacctctg aggtagaaaa ttatccagca gttcaaaaga tttctggggt
      601 agaattttct aataatatat acaatcaagc agttgctcaa ggtgttgata ttcagtttga
      661 tgaggttgaa gagataaatt tagagggaaa agtaaaggtt gtaaaaactt cttcaggtga
      721 acataaagca aaggctgtta ttcttgcaaa tggagttgaa agacgtaaat taggttgtgc
      781 aggggaacaa gaatttacag gtagaggagt atcttactgt gcaacatgtg atggagcctt
      841 ttttaaagat aaagaagttg ctatagtagg tggaggaaat actgcactag aagatgcttt
      901 atttttagct aataattgta caaaagtgta tttaattcac agaagagata gttttagagg
      961 tgaagaagtg ttggagaaat ctgtgaaagc aagagagaat attgagatac tttacagtca
     1021 tggtgtagaa aaaatagaag gagaaaagac tgtatcgaaa atagaagtaa aaaacttaaa
     1081 gactgaagaa aaaagaacta tagatgttag tggtattttt attgctatag gtcttaaacc
     1141 taataataaa atgtttgaaa atgttcttga tttggatgaa ggtggatata taatttcaga
     1201 tgagtcttgt acaacatcag tagaaggtgt atatgtagca ggagatagta gaactaagtt
     1261 tttacgtcaa ataataacag cagcatcaga tggagctatt gcagccgttc aagcagctaa
     1321 ttatataaat gttgaataat ataaatttca aataaaaata gccatataaa ctgaaataat
     1381 ttttcagtac tatatggcta ttttgttaat tagaggtaaa tgtacgtttt atttaccttg
     1441 tctagatttg ttatatctta atctatcatg ttcatttaag aatctttttc taagtcttat
     1501 agaatcaggt gtaatttcaa ctaattcatc atcttctata aactctagtg cttcttcaag
     1561 agtaaatatt ctaggaggag ataattttat agcatcatct gaaccagaag cacgtacgtt
     1621 actcattttc ttgtttttac aaggattgac taccatatca tcttttctgg aattcatacc
     1681 aattatcata ccttcgtaaa cttctacacc agggtctaca aacattacag ctctatcact
     1741 taaggcatta agagaatacc ccatagtaac accaggtcct tgtgctatta aaacaccatt
     1801 tcctcttgaa ggtatttctc ctttaaattc ttcaaatttt tcaaatgaac gaactaaagt
     1861 accttctcca cgagtagcgt ttataaattc acttcgatat cctaaaagac cacgagtagg
     1921 tgctaagaac tctattttta catagtctcc ttctattgac atagactcca tcataccttt
     1981 tctcatgttt aattcattta taatagtacc agagtaaact tcaggacagt ttacaacaac
     2041 tctttctatt ggttccatta gttttccatc ttctttatgc attaaaactt caggtttaga
     2101 aacaccaact tcataacctt cacgtctcat attttcaagt agtatagata agtgaagctc
     2161 tccacgtcca gaaactttgt atccatcagt agtatcaaga ggttcaactt taagaccaac
     2221 atttacttca agttcttttt caagtctatc tttcaaatgt ctagttgtaa cgaattttcc
     2281 actttttcca acgaatggag agtcgtttac taagaagttc atagaaagag taggctcttc
     2341 tatttttatc atttccatag gtagtggact atctaaatcg catatagttt cacctataga
     2401 tatatcagga ataccagcta taacaactat ttctccacta gtagcttcat caacttcaac
     2461 ttgttttaaa ccttcataca cagaaagttt tgatactttt cctttagata caacagaatc
     2521 ttctctacat atagcaactt gtgtattgtt tttaagagta cctttgtaaa ttctacctat
     2581 accaagtcta cctacataat catcatatgc aagagctgat atttggaact gtagaggttc
     2641 attatcgtaa ttaggataag cttcaacgtg atttacaata gttttaaata gaggagaaag
     2701 gtcttcacta tcatcatcca tttcaagttt agcaatacct tgtttagcaa taccatatat
     2761 tataggaaat tcgcattgct catctgttgc atttaagtct acaaataaat cgaaaacttc
     2821 attaactact tcttccgctc tctggtcttt tttatcaatc ttgtttataa ataatattgg
     2881 ttttaaaccg aactctaaag atttttgtaa aacgaatcta gtttgaggca ttgggccttc
     2941 actagcatca actaatagaa taactgtgtc aacagttttc ataacacgtt caacttcaga
     3001 agaaaagtca ctgtgtcctg gagtatcaac tatgtttatt ttgtaatctt catagtttat
     3061 tgcacagttt tttgaatata tagttatgcc tctttctttt tcaaggtcgt tactgtccat
     3121 aacgcagtct ttaactactt cgttctttct aaaaacaccg ctttgagaaa ggaaagcatc
     3181 aaccaatgtt gattttccag cgtcaacgtg ggctataact gcgatattta taattttatg
     3241 tttttgtgac atttttgttg ctcccttcga ttaaaataac ttattaatta tagcacaata
     3301 tattattagg tacaaggagg aatatattaa attgtattaa tatagtttaa attggataaa
     3361 actgttagtt ttgcatctga aaattattct gtttaaaaaa attttttaga caaataataa
     3421 ctttaataga taaaaagatt aataaaagag taaaggatga tatatgttgg aagtaaccaa
     3481 aataataatt aaatattttg caagtgaaag tgatactttt gtaaaagaag aagtttgtaa
     3541 ttttgaagaa cttcttaagt ggtctcataa atttattgaa ggtaaaaaga tagaagatgt
     3601 tttgtatata aagtctaaag aaaactataa agtaaatgtg attaagtctt tagaaattaa
     3661 ggaattggaa atagtgagaa aacaacagat accaccgtat atgtagtatt aataatatat
     3721 aaaattaata tgtaatactc gcaataggat ataaatggta tatatggtaa taatttatat
     3781 taaaaaaact tttataaagt ttatatttgg gtataaaagt attaattaga cattaattag
     3841 gaggaactta aatatgataa gagtatgtcc aaactgttca aatgtggatg taaataagct
     3901 aaaaactttg gttggagaag aaaatgtaaa gactggttgt ataggtcagt gtagagcatt
     3961 taaaaaagaa gctgttggaa taatagacaa tgagcttgaa ataaaggaaa cagaagcaga
     4021 gttttttgaa gcatgtaaag aataaatttt aaataaaata taataaatag agcatctaaa
     4081 ttgtataagc attaaacaat ttagatgctc tatttttata tatacattta attatgagta
     4141 tacttttcta ttaaatacaa ataaatataa ctaatacatt tatttattaa aaaaatat
//
LOCUS       DAGSWI010000221         4102 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.226, whole genome shotgun sequence.
ACCESSION   DAGSWI010000221 DAGSWI010000000
VERSION     DAGSWI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.226"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..191)
                     /locus_tag="MJF80_003725"
     CDS             complement(<1..191)
                     /locus_tag="MJF80_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0282328.1"
                     /translation="MIAVKKAYKFRLYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNL"
     gene            complement(831..1196)
                     /locus_tag="MJF80_003726"
                     /pseudo
     CDS             complement(831..1196)
                     /locus_tag="MJF80_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(1217..2296)
                     /locus_tag="MJF80_003727"
     CDS             complement(1217..2296)
                     /locus_tag="MJF80_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBZ0282329.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            complement(2350..3639)
                     /locus_tag="MJF80_003728"
     CDS             complement(2350..3639)
                     /locus_tag="MJF80_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBZ0282330.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     misc_binding    complement(3802..4013)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1322 a          808 c          460 g         1512 t
ORIGIN      
        1 tttaaattgg tcaaatcaga actacactgt ttataagtaa aagtttcttt attgttttta
       61 taaacatcta ttcttttagc aagatactta ttatatacaa atctacaaca tccaaaagtt
      121 ttgttaatta actcttgttg ctttttattt ggatacagtc taaacttata agcttttttc
      181 actgctatca cctcacttta attgtttttt gatacacttt ttaatgattt taatatatta
      241 tataatgctg tggtgaacaa tttattaacc acctatcata atattaacaa atgtaattat
      301 ttaaaacaat tatttagaag gttttatatt taattttaaa taacatattt aagaaccgta
      361 agtcttaact tttatcattt ttgaggttgt cgttcacata agctcgctac tacctatgca
      421 gttctcttat gaacttctta cactttcatg taagcacaga ctatatctta tccttcggca
      481 ttatccgtta aggtctaccc acttccacta ccaatagctt gtagtgtact tccctcaaga
      541 ggaatagtcg ttgaagtttc tcctattcgg agtttacctg ctgattgccc attttaaagt
      601 acttagggtt taaccttata ccatctaacc aattttttct actttcgtaa ctttcacact
      661 taggtttatt tcatccttat gttttagttt ggttagcttt aggggtttcc agcaattcaa
      721 gtagtattgg ataggttcta tcctatctct acatataggt ttccctatat gctgactatt
      781 ttagctacta actcatgact aaagtcacga gtgtgcgtag ctatttttta atcaaactca
      841 tatccaccag ataccaatat tgaatctaat tctatttttt ctcttaagtc ttctggccaa
      901 tctataaatg gaagaatggc agtagtggct ggctttccta ttcttatcat agccacatta
      961 ttaagtcttt ttctttctac aatagcattt gtacaatgag ttgtaccaag catagcatat
     1021 tttattaatg aatggtcaat cttactttca tctataactt tttttaaggc attgtaaaca
     1081 ccagtttcaa catcttttgt agttgccacc ttaactgaat gaattacttt taattcccca
     1141 tccagtatac aagcatctgt atttgttcct ccaacgtcaa ttcctacacg atacatcaaa
     1201 atttcctcct aaacttttat tttccttgta attcttctac tgttttatat tccacatcat
     1261 atccaaagta tccaggtcca actgtctcaa ttcctttttc tgttctccac ttttcattac
     1321 aaggaaaagc tactaccata acacgagaac catattttat tctctctgta gttattggaa
     1381 atccagtttc taaatctaat actgctatta aatctggtgt aatacaaagt ggtgtatcac
     1441 cttttttggc aattaaattc tcattttgga ataatataga atactctcca tcatactcat
     1501 taatcccagc aaaaaaggca tgtcctctag taaatccacc tttcaactct ctcttaatat
     1561 caattacttt tccttcaaat aacttgtatc cacgcaattc ttttagtata ttctcaacag
     1621 caccttttga atcagattta ttttcaagta aaaaccctcc tatagtttca gctattgtaa
     1681 gagtattacc tatagcagct tttttaactt gagctccact taaattatag tcactaatag
     1741 aagaacttcc tcccatttct acagttaaag ttctagctaa tctttctgac caaattccat
     1801 ccacaggata aaggatggca gaattacctt tttcatcaga cattacggca ggagatgcct
     1861 catatccttc aagataaaaa gttaccattt gagcttcagg gaacgctcta cccatagaat
     1921 ctgcatctaa gataggaagt ccttttttag cagcaactgc tataggcata agtgagttta
     1981 tccctcctat ttctatagga attataacat ccacttttct atttaataat ttctccatta
     2041 tttctaaagg ggtagttaac tctacttctg aaagtaattt ttctattgta attacaggag
     2101 aacccatacc agatactgga actaccaaag catcatcatc aagttcatct aaacttatta
     2161 cttttacagg accatacttt ttaatggctt gttttgcaac caatttacct acatatgggt
     2221 cacctccacc tccagttcca agcactgctg caccaactgc tattttatct acactttctt
     2281 ctgttaaaaa tctcatgtta ctttctcctc atatttctaa tttattttat actttataaa
     2341 caaatttctt tatactgctt tgtctatttc ttgcttattt tttgttgaac aaagcacttt
     2401 tccaataata gattgtacta caaaccctgc tataaaacca tctaaaggtg ctatagtagt
     2461 aagagttata aatccatatg ttgaaaggta agttattaat ataccaatta tccaagacac
     2521 tattgaacgt ttaactattg gtttatataa tactccttta tcaaagcctt taagttcttg
     2581 ctttactatg taatattctg ctgtatatac accaccaaca ggtgcaatta caattccaag
     2641 taaatttaaa aatcctataa aaccttcata tattccaaat acagccaata atgttgctat
     2701 aataccaagg actatagtta atactttctc aggagccttc ttaagtacaa gagctatact
     2761 taatgatgct gaatatacat tacttgtatt tgtagtccat tgtgctaatg taagtactat
     2821 tatacctggt atagcaattc ctaatgtaat aaaaattctc ataatatctg aagttcccat
     2881 acattttact aatactatcg aaactattat cataaaagca tttccaaatt gtattcccaa
     2941 aaagcttgaa agagcacagt ctcttctact tttagcccaa cgacttatat caggagatac
     3001 aattgcacca actattatta tactcattga cagagaaact ccacctgcaa atgtcatagt
     3061 agatttcata ttatcatcaa ctgaaattcc atttgctcta aaagctaaga atatagttag
     3121 catcataaga actaataaaa atggtactga atacacactc aacttctcca ttgccttata
     3181 tccataaatt gcagtactca tcatcaagag accacctatt aggcttaaaa tctgcatact
     3241 gacatctaaa ttaaatatat cttttattat tatctgtgca ttttgagcaa agaaacctac
     3301 ttgaactcca aaccaaccta atagagaaac ccctataact agagaaaata tttttgctcc
     3361 agcttctcca aatacatact ttgttatcat agatgtagat aaacttgtag atgaaccaac
     3421 caaagagcat gtagtataca aaattgcaga aaaaaatgaa ccaacaatta ctgacaatat
     3481 tgcttgtttc attggcatac tctgtgccaa ctcagcgcct aaaataacag atgataaatc
     3541 cacacctatt gctactaaaa ctgaaaacat agctacccaa ccacgttttt gattctctgg
     3601 aatctgttcc agagcatagt taattttttc ttcttccatt ttcatccctc catttaaatc
     3661 gtaaaactac tgaccttagc atattttaca attttctaat ttataaattt attaactatc
     3721 atatttttat aattttcatt taatatatag ttttaaattt tctgaaataa gtttataata
     3781 aaaaacgcct cttggaaaaa tccaagagac gaatataaat ccgtgttacc actcttatta
     3841 gtaaaaatta attttactca ctcattttct taaggcgaat taaaccacta ttctaacata
     3901 attacaatag tattctccaa agtccttttc acataaattc caatattagt tctcagccaa
     3961 atctaactct ctgtaaaatt cctttatgct actttcttct tctacgaata tatcatattg
     4021 atttgtagtt aatttatgat ttatatcata atatatatgc aatattttgt caacttaaaa
     4081 agtcaattac ttgcattttt ta
//
LOCUS       DAGSWI010000222         4097 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.227, whole genome shotgun sequence.
ACCESSION   DAGSWI010000222 DAGSWI010000000
VERSION     DAGSWI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.227"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            383..1060
                     /locus_tag="MJF80_003729"
     CDS             383..1060
                     /locus_tag="MJF80_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282331.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     gene            1132..3147
                     /locus_tag="MJF80_003730"
     CDS             1132..3147
                     /locus_tag="MJF80_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBZ0282332.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILFPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFEAYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            3197..3877
                     /locus_tag="MJF80_003731"
     CDS             3197..3877
                     /locus_tag="MJF80_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282333.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
BASE COUNT         1637 a          409 c          674 g         1377 t
ORIGIN      
        1 gagatgcaga atataaacct tttgtaacaa tatatttacc aaaataatac aatttatttt
       61 atattataac catataaatc atataaatca atactcaaac tagccaatag acaagtttct
      121 agaaaaataa taaatttata cgtgaaaatg actttaagct ccattctcca acaatcggag
      181 actttaatta gtatttaata atgaatctta atgtttaata caatattcaa gaattattca
      241 aaaatactta agtattatag tctagtttgt aaagatatta taaaataata ataccagtta
      301 tatatgaaaa taaaacatta taatttgtaa taactataaa acttggaatt ataattaaaa
      361 tacctttgaa tggagtggtg taatgtataa tttactggtt gtagatgatg atgttgatat
      421 tttaaacata aatcaaattt atttttctaa tataggtttt aatgtatata catcagagac
      481 tgctaaagat gctatggata tagtagagag tatagtttta gactgtataa ttttggatat
      541 ttcattgcca gatatagatg gatataaaat gtgcaatgat ataaaagaaa aggttaacat
      601 acctataata tttttatcta attatgagca tgaagaagaa aaagttcggg gatttttagc
      661 tgggggggat gattatataa caaaacccta tagtttaaaa gaacttgaac ttagaatata
      721 tgctcgtatg aggcagtaca aaaatattac atcagttcct aatgtgttaa agttttcagc
      781 gttagtaata aatgtaaatt caagaaaagt aacttttgat ggagaaagta tagatttaac
      841 tactatggaa tttgaaatat tattgtttct agctgaaaac aaagaacaag tattttcgaa
      901 gatagagata tataaccaag tttggaaaat gcctgatatt ggagatcaac atacagtgca
      961 ggttcatatt gcacagatga gaaaaaaaat taattcttta agcagggagc atcaatacat
     1021 tcaaaccgta tggggaaaag gatataagtt tgtaccataa gtgagatact gtaattaata
     1081 aatagttctt gttttattta atactactat gaaagagatg tggttgatac aatgaaaaag
     1141 tataatatac taaaaggaac tctcattgta ttgatagtct ctctgttggc aataacagga
     1201 ttaaatcttc tgtataaata tgataataaa tatacatata agtctattag agcaagtaat
     1261 ggaattttaa ctatagataa tgatactttc actaaaggta aacttgtctt tttgatagat
     1321 gactgggagt tttataatca taaaatattt aaaccgaatg attttacaga gcaagatata
     1381 aaaccagaat atgtttttat aggtcaatat ccagattttt ctatgagaaa ggggactcag
     1441 tcaccatatg ggaatgctac ttatcgtatg agaataaaaa atgaaggcaa ggataagcta
     1501 ctaagtttag agcttcctga gattttttcc gcaagtgaag tatggataaa tggagaaatg
     1561 gtttctaaat taggagatgt aggaacagaa aaatatagac ccaaaattaa aaattcagta
     1621 gtaagtttta ttgcaaagaa aaatacagaa attataatca atgtatcgaa tttttcacat
     1681 tattatagtg gtttgtatta tccgcctgca ttaggagaaa caaaggacat aagtaatatg
     1741 atgttttatc gattactttt ttatagtata atttgcttca caacttttgc aatagctata
     1801 ttttcattgt ctgtatggtt tctttcaaac aaaagtaaaa tttacttata ttttggatgt
     1861 atgtgtgttt tctttgccat ccatgtatcc tatccctttg tacatttgtt aggactgcct
     1921 ttggtaagct ttacatatgc ggttgaagat ttgtcatatt ttatggtagt tttatgtatt
     1981 atttgtataa atggaagtat atccaaaata gaagaaaata aagtgtacag atttttaatt
     2041 ttaccattag gaattacaat gagtatcata tctatagtga taccaattat tctttttcca
     2101 tatgatatgt actttgtcaa catatatggg aagttaatag acatatataa atatattgta
     2161 tttatatata tattaatgtc ttcgctggtt tcaatatata aaaagacatg ctctgagcat
     2221 ataagttctt atattttagt ttcagtaaac actgtttttg gaattagcat attatttgat
     2281 gcagttacga gtaatagatt tgagcctata tttacagggt ggcaaacaga atattgtggg
     2341 tttgtaattg ttattttatt ttctgttatg atgataaaca ggaatagaat aataataaaa
     2401 gagaatgaaa aacttacaag gaatcttgaa catgaagttg aagtgagaac taatgaactt
     2461 acaacattgt taaatgaacg taaaagattc ttggcagatg tagcacatga tttaaaggca
     2521 cctgtttcgg ctatacaagc atttatagat ttgataaagg ttggtaatat tcatgtagat
     2581 gaagagacaa gaacctatct ttatgctata aatcaaaagt caaatgaagt acaaaataga
     2641 gtacgtagtt tgcaagaatt tacatcacaa gataaaagca ttggtattag tgaaaaaata
     2701 tgtctgaata attttattaa agagatatat gaaaataatt tgccagatac acaagcatgt
     2761 ggtgtaaact ttgaagctta cgtattggag aaaaaaatat atgcaaagat aaataaagag
     2821 atgcttacaa gagcatttga gaatttaatt tataatgctt tgacctttac tccatttgat
     2881 ggaaagataa ttttatcaat ggataaagaa gatggatttg caattattaa aatttctgat
     2941 aatggaaaag gtatacctcc agaaaatata ccaaaggtat ttgacagatt ctttacagaa
     3001 agagacaata atgagccaaa agggcaaggg ttagggctat atattgtcaa atccattata
     3061 agagagcatg gtggagaaat ctttgtagat tcagaactag gaaaaggaac tgtttttaca
     3121 ataaaattaa gtctatgttt tttataataa tagcttattt aatataaaag aataatgata
     3181 tttgggagtg attataatgc aaactaagat attgataata gatggagaca aggataattg
     3241 ccagaagcta aaaggatttc tggaggaaaa aggaataagt atagatttag catataattg
     3301 tgaagaagct attggaaaaa tatttagcaa taaatatgat ttaatatttt tagaaataat
     3361 tctaagtgat ggtgatggat ggactctttg taaaaaaatt agaaatgtaa ctacttgtcc
     3421 aattgtttat atgacttata taaatgaaga ccaaagcatt ttaaatgctt taaattctgg
     3481 tggagacgat tacttaataa agcctctaaa tttagaaata ttatacgcaa aagtaaaagc
     3541 tattttaaga agaatgaact catatgtaaa taataatgaa aataatacaa aattagagga
     3601 atataataga actagtggag taataaagtt agaagataaa ataataaaat tgactccaac
     3661 agaaaataag ttgttaaact tttttataga gaatcctgaa aagactttga ctactaaaga
     3721 gatatatgaa catgtatgga tgaatgaata cttagaggat aactatagtg tggtagttgc
     3781 agtgaatgga ttaagaaaga aaatagagaa agattataaa aatcctcaga aaattataac
     3841 aataagagga gcaggatatt attttaataa agaataataa atatattaat atcattaata
     3901 attgttataa atgtgaaata ctaacgatat ctaaactaaa ttattataaa atagctgact
     3961 gtaatttaat tgataaagcc tattcttcta gactttgcca attaaattac agtcagtttt
     4021 attatataaa aaaatgcgta acagaactat tattttatag attatatttg aaaagataaa
     4081 taaattgtat atctaaa
//
LOCUS       DAGSWI010000223         4060 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.228, whole genome shotgun sequence.
ACCESSION   DAGSWI010000223 DAGSWI010000000
VERSION     DAGSWI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.228"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            401..1294
                     /locus_tag="MJF80_003732"
     CDS             401..1294
                     /locus_tag="MJF80_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBZ0282334.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1294..2688
                     /gene="gltA"
                     /locus_tag="MJF80_003733"
     CDS             1294..2688
                     /gene="gltA"
                     /locus_tag="MJF80_003733"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBZ0282335.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIVF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3054..>4060
                     /locus_tag="MJF80_003734"
     CDS             3054..>4060
                     /locus_tag="MJF80_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HBZ0282336.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNVKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     K"
BASE COUNT         1607 a          400 c          772 g         1281 t
ORIGIN      
        1 agccttatac acgtttagtt tttaaagaaa ttagtgttat tatgaaaacc taagttaatt
       61 tattaaataa ataattgaat aaaaaaattt tattatgtat ttataaaatt tttgttacaa
      121 atttggcata atatagtata ctttaagtgt gtattaggtt aaatctataa caaattagac
      181 gtatgatata gacttagtgt ttattggcat ttacaagaaa tttgttaata tcaactattt
      241 gtagtttggg cagttataat tattataggg gggatgtcca atacaaaaca atcaaccaag
      301 aactaatatt atattataaa gagttatagt tttgtgtgtt tatgttttta attaaattta
      361 aagtgggaaa aatatatttg ttagaatgga gaatagcgag atgagtaata aaatagttag
      421 taagagacaa ttaacagata gtatttattt aatggaaata gaggcaccta gagttgcaaa
      481 atcttctcaa ccaggtcaat ttataataat taaaaatgat gaaaaaggag agaggattcc
      541 tcttacaata gctgactatg atagagaaaa aggaacagta actattgtat tccaaacagt
      601 tggtgcttca actaaaaagt tagcaatgtt tgaagaaaac gattttgtaa tggattttgt
      661 agggccatta ggacaagcaa gtgaatttat acatgaagat atagaagaat taagaaataa
      721 aaagatatta tttgtagctg gtggagttgg ttctgctcca gtttatccac aagttaaatg
      781 gtttaaagag catggattag atgtagatgt aataattggt gcaagaacta aagaattaat
      841 tatattagaa gatgatatga aaaaagtagc taaaaatgta tatgtatcaa cagatgatgg
      901 tacatatgga tttaatggaa gagttacaga cctattaaaa gatttagttg ataatcaagg
      961 taaaaaatac gaccaagcaa tagtaattgg accaatgata atgatgaaat ttatgtgcca
     1021 attaactaaa gaactaaata tacctactat agtaagttta aatacaataa tgatagatgg
     1081 aacaggtatg tgtggtggtt gtagagttag tgttggaaat gaaactaagt ttgcatgtgt
     1141 agatggtcct gaatttgatg gtcatttagt tgattttgat caagcaatga gaagacagtc
     1201 tatgtacaag actcaagagg gaagagctat gctaaaactt gaagaaggcg atagtcatca
     1261 tcatagtaat tgtggatgtg gaggtaacaa ataatggatg ctaaaaaagt aaaagtacca
     1321 gttagagaac aagaacctgc tgtgagagca acaaattttg atgaagtatg cttagggtat
     1381 aataaagaag aagctatggc agaagctaat agatgtttag cttgtaaaaa accaaaatgt
     1441 gtgggtggat gtccagtagg aatcgacata ccagggttta taacaaaaat taaagaagat
     1501 gatatagaag gtgctgctaa agtaatagct aagagcagtt cattaccagc tgtttgtggt
     1561 agagtatgcc cacaagaaag tcaatgtgaa ggtgtatgta tacttggaat aaaatcagat
     1621 gctgtatcaa taggtaaatt agaaagattt gtagcagatt ggtcaaaaga aaatgacata
     1681 aatttatctg atacagagcc taagaaaaat caaaaagtag cagtaatagg aagtggtcca
     1741 gcaggtctag cttgtgctgg tgatttagct aaaaaaggct atgatgtaac aatctttgaa
     1801 gcaatgcatg agccaggtgg agttttaact tacggaatac ctgagtttag acttccaaaa
     1861 caagctgttg ttcaacctga aatagacaat ataagaaaat taggtgtaaa aatagaaact
     1921 aatgtaattg ttggtaaaac aattacagta gatgaactta tagaagatga aggatttgaa
     1981 gctatattca taggttcagg agctggtctt ccaatgttta tgaatatacc aggggaaaat
     2041 gcaaatggag tattttctgc aaatgagttt ttaactagag taaacttaat gaaggcttat
     2101 agagatgatt atgatacacc tattagttct ggtaaaaaag ttgctgtagt aggtggtgga
     2161 aatgttgcta tggacgctgc tagaactgca ttaagattag gttcagaatc atatatagtt
     2221 tatagaagaa gtgaaaaaga gcttccagca agagctgaag aagttcatca tgcaaaagaa
     2281 gaaggcattg tatttaatac attaacaaat cctaaggaaa ttttagtaga tgaaaatgga
     2341 tatgttaaag gtatggtttg tataagaatg gaattaggag agccagatga ttctggaaga
     2401 agaagaccta tagaaataga aggttctgaa tttgtacttg atgtagacac agttataatg
     2461 tcacttggaa caagcccaaa tccactaata tcttctacaa caaagagtct tgatataaat
     2521 aagaagagat gcttaataac agatgaaaat ggtcagactt ctaaagaggg tgtatttgca
     2581 ggtggagatg ctgttactgg agcagcaaca gtaatatctg caatgggtgc tggtaaaaca
     2641 gcagcagcat caatagatga atatttaaaa gcaaaagtta atgcttaatt tgtacattaa
     2701 agagagggtg tctcaaaatg aactttttag ttcatgaggc actctttttg tacaaaatca
     2761 aatatgtttt aatcatttca acaatgaaaa tttcattcct gtagaaagtc taacttttag
     2821 gtcttagtac aatctctatt ttgatgtagc cactttttta gatggtttaa taatttatca
     2881 gttattattg taatatttga tactaagtaa cataactttg tctaactatg ataaaatagt
     2941 tatacaataa aggtcttgta aggagtgaat aatgtgtggc taataaaatc agatttgtca
     3001 aatctaacat taaataatag aaaaataaat tgtgtaatta atggtatttt aatatgaaaa
     3061 aaagagaaaa agtatttgat gggctagttt ttaagcatat attaatctta ttaatactat
     3121 tagttttgat tgatttaaca agtaccatca tatatgcaag taactctatg gaaattactt
     3181 ctagaaatat gattgaggca tcaaaaagag aacttgaaaa ctatttagaa gtaaacatat
     3241 ctttattaaa agctcttagt caagatgata gattttctaa tggtgaaaca tcactaattg
     3301 aaaagggaaa attattaagt ccatatcaaa aggaatataa tctttttatg attggtataa
     3361 cagatacaaa aggaaatgct tcaagtacat atagggaaaa tgttaactca ataaaagata
     3421 agcctagttt tgagaaagca attaaaacaa agcaagtagt agtttcagat attgaagtca
     3481 gtaatgtaac aggtgacaaa gtatttatca tatatgtacc aattgtaaaa aataatgaga
     3541 tgattgggac catttttgct tctttttatt ttcaagatgt aaataattta ataagtagaa
     3601 gcaattttga tgattctatt aaatttctaa tgatagataa agattatact attatttctc
     3661 atccaaacaa aaaatatgta aatgataaaa gtaagatgct tgatttagaa gggaatatta
     3721 taggaaccac taaaagtgaa atcttaaaaa atataaataa aaaatgtcaa ggtgattttt
     3781 tatcttggga taattggaga ttatataatg taaaatatac gaacgtaaag tggactaatt
     3841 ggactttagt atcaaaatgt aatatattta agaactttaa aaatctaata gttaatttta
     3901 tgataaagtt gtatttttat atagtaatat ttatgatatt gtggaaatta agtaatgcta
     3961 aattaataga acaacttaaa aaattggctt attatgattc tttaagtgga ataaaaaata
     4021 aagaaaagtt tagaaaagat tctatgtata ttttaaagaa
//
LOCUS       DAGSWI010000224         3994 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.229, whole genome shotgun sequence.
ACCESSION   DAGSWI010000224 DAGSWI010000000
VERSION     DAGSWI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.229"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            124..996
                     /locus_tag="MJF80_003735"
     CDS             124..996
                     /locus_tag="MJF80_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282337.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
     gene            993..2282
                     /locus_tag="MJF80_003736"
     CDS             993..2282
                     /locus_tag="MJF80_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282338.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDHILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLEALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            2212..2856
                     /locus_tag="MJF80_003737"
     CDS             2212..2856
                     /locus_tag="MJF80_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282339.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3123..3587)
                     /locus_tag="MJF80_003738"
     CDS             complement(3123..3587)
                     /locus_tag="MJF80_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0282340.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIISLRLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
BASE COUNT         1506 a          468 c          600 g         1420 t
ORIGIN      
        1 atctttagaa aaaatttaga aatttataaa taatatctta agttttagaa tttattatga
       61 aaatacagta aaaatttata aaaataaaat tctataaaat tatttctggg ggtgtattta
      121 tatatggaaa tgaaaatagc agtatccaat cttagtaaaa gatttgggaa aaaacaagct
      181 cttgataata taaatcttga aatcgacagt ggtatgtttg gtcttttagg aagaaatgga
      241 gcaggaaaaa ccacatttat gagaatttta gccactttat tatcaaaaac agaaggttca
      301 atacatatgt gtggagttcc tattgagaat actaaggaaa ttagaaatat ggttggttat
      361 ttgccacaag atttttcaat gtatcctaac atgacagtat ataaagcaat ggattattta
      421 gcagttttat cggatatacc actaagaaaa agaagaaaaa ttatagcaaa gttattaagc
      481 aaagtaaacc ttactaatta tcaaaatgtg aaagtaaaat ctttatctgg aggtatgaat
      541 agaaggcttg ggatagcaca agcattaatt cataatccca gagttttaat agtagatgag
      601 ccgacagcag gtcttgaccc agaagaacgt ataagattta gaaatttact atgcgaaatt
      661 gcaaatgata gaatcgttat tttatctaca catattgttg gagatataga agctacatgt
      721 gaaaacgtag ctatattgaa tagtggaaaa gtcatataca atggctcaac agaagcatta
      781 gcaaaactag ctgaagggaa agtatatagt attgaagtag ataaaaaaga cattgaaaat
      841 ataaaaagta gatttatagt tataggaatg ttgactcatg gtggaaaggc tatacttagg
      901 attatatcag atgataaacc ttttgagaca gcagtaaatt gtaatccaac tattgaagat
      961 ggctatatgc ttataatggg aggagataat atatgaatct atttataaaa gaatgtaaaa
     1021 atatattaaa gagtatggta tattatattt ttattgtagt tttagttttt ttctatattt
     1081 ctcaacttgg aagttcagtt tcaaatgatg taagtaaaaa tattcctaat gatggaaatc
     1141 cattagttgc tccacaaaaa gtagagaata ttgaggaata ttattctgta cacaatcaat
     1201 atccatatgg tcaaaaagaa tctatgaaaa cagaaaaggt cattccaaat gcaactataa
     1261 acctaattag agaatatcaa aaaaacacat atacaacgta tccaacaggg gtagttaagt
     1321 atataaaatt agaccaaaat aaaacgaaga ccatagaaaa atatatagaa gaaataactg
     1381 gtaaaagtat tagcgaaata aattcacttt ggaaaactga tgaaagtata aaaatagatt
     1441 attctaaagt atctcttgaa agatttgaag aaataatgca aagagtagac cacattttag
     1501 gaggagggtc tgcatatgct tttgacaagt tacaaagatt tggagtagtt ccagttacct
     1561 atgatgaagc tatgaagaat tacaactata caatacaaaa agataaagtt agtaatgggt
     1621 atgcaagaat tttttgcgat tatataggta tagttttaag tatatttcca gtatttgtgg
     1681 cagtttttat gtcacttaaa gataagcgtt caagaatgag agagattatt tattcaaaac
     1741 aagtatcttc atgcaaaata gttttatcaa gatattttgc acttgtcttt atgatgatat
     1801 tgcctgtatt aattttaggt atgaaagatt tacttccttt gattttcttt gctaaagata
     1861 aaggtatatc tattgataac ttagcattta taaaatatac attatggtgg atactaccaa
     1921 ctcttatgat agttacagct gtaggaatga gtattactat attaacagat acaccagtag
     1981 caattattgt gcaattaatt tggtggcttt acagtataaa ttctacagga ctcaaaggtg
     2041 attattcaac aatgggacta atgataagac ataatagtgt attaaatgga gatattatta
     2101 tacaaaactt caatcaaatt atgattaata gaattgtgat aagcttggaa gcattggtgc
     2161 ttgttttgtt agctatattc ttatatgagc aaaaaagaag ggggaatctt gatgtttggg
     2221 caaagttttc aaaactgttt agttctaata aaagaaaact tcaaattaag cactttaact
     2281 aatttaatat gttcaattat attgctatgt atgattcctc taatacaagg agtagctaat
     2341 ttagacagaa tttcaacagc tatatgttta gaaaattatg ttgcaataat agggatagta
     2401 atgttggtac ctgtattttc gctagagcaa tcaaaagaaa ttgatgagat agtatcttca
     2461 aaagcattat ctcaagttat tgtacaatta attaggatac tgatgtcaat tataggtata
     2521 gtggtattaa tcataggttt tgtaaatgtt ttaatatatc ttggatgtaa ttttccagta
     2581 gttgagtata ttattggaag tttcgcatca gctatgttta taggaagttt aggaatgttt
     2641 ttctcaaatt tatttaataa tacaatcata ggttatatga tatctatagg atatttaatt
     2701 ttaaatatga tgactaaagg caagtatgtt ggaaaatttt ttataatgtc tatgagtcaa
     2761 ggtagttttg atgagaaata ttggctgata cttggaagtg ttgtgttgat aattgcatct
     2821 ctcttaataa aaccagtaaa aaggaagatt agataataaa atgagagctt agataaaata
     2881 aagttaatac aaataaaagg tacatttatg aaatataaat ctatttttat atacacaaac
     2941 ctacttacaa tctctttatg tatgtagata gatttatata tccgtagtat aaaatatata
     3001 tgtataccaa attttatcaa aagctctcaa atttatattt caaagacact tattgctatg
     3061 acctacacat atgtattttt ttattttaat tgttgtatta acaataagac tattgttata
     3121 aactattgat acctcataac aaaaaattct ccatctccta aatgcgtttt ttgtacctta
     3181 gtctcaacta ttttaaatcc ttgagactca taacatttta cagctctcat gttccaggtt
     3241 cttacattta atacaattgg tttagtacta tatttatttc tacattcttg taatcttagg
     3301 ctaattattt ctttaccaat tcccattcca caataattcg gtttcatacc aataccaaag
     3361 aatacagagc ttccttcatc taataagttt ataaaaccta tcaattcttt attgtcattt
     3421 ataaaactta taaaattttt tcttttatct tcttttgcta gtgaaaaatt cttcttttta
     3481 atttcctccc atggaggtag attgtatatt gaatatttac cttcatattt ccaattagta
     3541 acttcaatag catgttcttc agtaaattta actttattta aattcatatg tcctcctatt
     3601 aaaatattta aggtaattaa tggtatttta aagttttaga caataagtaa aactacttat
     3661 atttatccat aaaatcttta aaaattttac taacaattct tgtaccctgt actttttatt
     3721 ggattcacag aagactataa actaattttg aattaccttc taattcaaaa acatcacgat
     3781 atatataatt tcttagcttg attaaattct gttaaatccg tttcttatag ttgtataatc
     3841 aacatcaata tctttatgtg tattagttaa tttattggct ttaggaactt attattaata
     3901 tattgtattt actccaagta gtaggtaaat tataggcaaa ttatcaaatg tatatctatc
     3961 catgcttaaa ctttagagat acaataacta ctaa
//
LOCUS       DAGSWI010000225         3953 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.230, whole genome shotgun sequence.
ACCESSION   DAGSWI010000225 DAGSWI010000000
VERSION     DAGSWI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.230"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..1730
                     /locus_tag="MJF80_003739"
     CDS             <1..1730
                     /locus_tag="MJF80_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBZ0282341.1"
                     /translation="KKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFEKMEYK
                     RPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNNIDSSD
                     EFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIGKLNSK
                     EVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYSDYLKK
                     YNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVKDYIVP
                     LYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDYSSGKN
                     KSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQPSIDV
                     CEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIYKNPYM
                     SLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDVSLQIW
                     QLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYDYFGID
                     NPLELKNAS"
     gene            complement(1900..2088)
                     /locus_tag="MJF80_003740"
     CDS             complement(1900..2088)
                     /locus_tag="MJF80_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282342.1"
                     /translation="MKIITIGSSLITVLLFLSTMICGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYI"
     gene            complement(2085..2666)
                     /locus_tag="MJF80_003741"
     CDS             complement(2085..2666)
                     /locus_tag="MJF80_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0282343.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2834..3652
                     /locus_tag="MJF80_003742"
     CDS             2834..3652
                     /locus_tag="MJF80_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBZ0282344.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
BASE COUNT         1505 a          512 c          538 g         1398 t
ORIGIN      
        1 agaagaaaat atttgttttt ctaattttaa ttgcagtttt tattatattt gcatcataca
       61 taagaaatta tactaatgat acaaaaaaag attctgttag aggaactatt ccttttgaaa
      121 aaatggaata taagcgtcct aatatacagt caatttgcga aaacattaac aagtgcaacg
      181 acaaactttt aacagctaaa acagctaagg aacaattaaa tttatttaat gaagttgata
      241 aaatttatca agatttttac agtacactta ctatagcaaa aattagaaat aacatagatt
      301 catcagacga attttactct aaggaatata agtatcttat gagtaaatca gtagatgttg
      361 acatgtcata caaagacttt caagatagat ttgtaacttc taaattctct aaagaattag
      421 aaaaagaact tggaaaggat acatttaatt atctaaagaa cattggaaaa cttaattcta
      481 aagaagttga aggtttgctt aaaaaagagc aagacctagt tgtaaaatat gaagacttat
      541 tagctaaatc tacagtatca atagatggta tagaggtaga ctttgaagaa gccttgtcaa
      601 gaccaaatct tagccctgaa gaatatgtga aaatttattc agattactta aaaaaataca
      661 atcctatatt tggtaacata ttcttagaac ttatacagac aagaactgaa atagcaagta
      721 agcaaggatt taagaattat atagattatg cctatatgaa cctaaacaaa gactattctc
      781 aagaagaagc taaaaaattt agacaagatg taaaagatta catagttcca ttatatagag
      841 agatttcatc taaaccatct gattcttcta tttatattaa agtatataaa aatcgctcat
      901 ttaggaagtt tgataaagta cttgaagata tatctccaaa actaaaagag agcttcgact
      961 atatgaaaaa atatgacttg tacgattatt cttctggaaa aaacaaatca cctggaggat
     1021 atactactta tataaataaa tataaagcac ctttcttgtt taatacatgg gataattctt
     1081 ttttaggtgt tactagtttt gctcatgagt ttggtcattt ttataattac tacaacagta
     1141 ttaacttaaa caataagatg caaccaagta tagatgtatg tgaagttcat tcaacatctt
     1201 tagaaattct attttataaa tattttgatg atttttttgg taaacaatct gaagctataa
     1261 aaaaagaaca cttatcgatt gttttaaata ctattattga tgcttgtctt tatgatgaat
     1321 ttcaagaaat tatatacaag aatccatata tgagtctaaa tgatatcaat aaattgtttt
     1381 ttgatttaga ggaagaatat ggagtttcca ataatatact tagagaaaaa aatgctcctt
     1441 tttggatact tgtatctcat aacttccaag ttccttttta ttacttgagt tatggtcttg
     1501 catctgatgt atcacttcag atatggcaat tatcacaaga agattacaga aaagctgttg
     1561 atgtatatat ggatttttta aatcaaaata ctgatgctgg atttaaagat gtggtagaaa
     1621 aggtaaattt acaattacct ttccaagatg gaaatttaga ggaaataagc tctactttat
     1681 atgattattt tggtatagat aatccgcttg aactaaaaaa tgcttcttaa tttctaatac
     1741 tttatactca ctcttaagat ttagtattag attgaaataa gtgatatttt ttcacacaca
     1801 attaatgttt aataaaatta tatggtaaag tatcagaaat ttttaagact cttgtcttta
     1861 taatttctga tactttattt attaataata ttattatttt tatatatata taattaaaag
     1921 gattgtggaa atcaataaaa atatccctgt aaaaatagca ctattcatat ggaacttaat
     1981 agaacttgca tctgttacct tattattctt aatccagaac ccacaaatca tagttgaaag
     2041 aaaaagtaaa acagtgataa gacttgaacc aatagttata attttcatct tacatcaaca
     2101 tcctctccaa taatattttc tattaaaata tcaatgaatt tatctcgctc atatttattc
     2161 tttatattta taccactgta taactgaaat gattctgtag ataaagcaga aatctgtatt
     2221 ggtaaaataa tttgcagggc aataattctt agactcattt catctttttt atttccaaaa
     2281 attttacgaa gcatagggac aatatcaagt tctgcttgca tatctccatt ggttatacat
     2341 tgattcaata caaaaggaag cactttttca taatgtaatc cagtatcaca caattttttt
     2401 aataaatttt ttaaatcttc aataggtctt agagtatata cactatcgtc atatagctct
     2461 gctattatat ttgacatcac atctcctatg gcagtactca acaaattatc ctttgtttta
     2521 aaatgataat ttattaaacc aactccaaca tttgcacgct cagcaatttt tctaactgta
     2581 atctcctcaa tatcatctac ttcatttaaa agattcaatg taacttcaat tatacgttct
     2641 ctagcatctt tatcattact tttcataata gacctccaaa acaattaaca ttttcagttc
     2701 tgaacatgtt cagtataata tttttttcat catatgtcaa tataatttaa ttataatttt
     2761 agttgactct aacattatga tatggtttat attaatatta agctaaaagt acctttataa
     2821 aggagacaaa aacatgttta agataggcga tttttcaaag ctaagtaaaa ttagtattcg
     2881 tatgttacgt cattatgatg aaataggact tttaactcct tctcatacga ataaaactaa
     2941 tggatataga tactatagtg cagaccaact ttctactaca aacagaatcc atgcactaaa
     3001 agatatgggc tttggcttgt attctataaa agaaattttg acagaatata acgataaaga
     3061 aagtctcata aaatatctaa atatccacca ttctcaagta aaagaacaat tagaagatac
     3121 acagaaaaaa ctattaaaaa tagaaaccac tattaaaaga attggaggaa atgatattat
     3181 gaaaaattat gatgtaacta taaagaattt tgcaccaaaa tatatgatga cacttagaag
     3241 ggttatacca acttatcaag acgaaggaat gttatggcat caagcatttt tagagacaaa
     3301 agaccagaat gttcaaattg aacccccaaa gtattctaaa gcagtttttt atgatactgg
     3361 atataaagaa gattatgtag atgttgaagt acaagttgct gtatctggaa agtataaaga
     3421 tactgaacat gttaaattta agactgttcc aagtgtgact gctgcaactg caattgttaa
     3481 tggaaatttc aatcaagttg cagacgcttg tgaggctatt ggtaattgga tatcagataa
     3541 taactatgat gtggatggtc ctatgtttaa tatttatcat gtaagtcctg gcaacgatag
     3601 taatcctgat aattgggtaa cagaggtatg cttcccagtt aaaaagaagt aatttttata
     3661 ttaattggat aatattttaa tttgttagac aaattacttt tagtgttatg tataaatacc
     3721 tacaatttaa ttaggatatt atcttaattt gttaaacaaa ttacttttag tgttatgtat
     3781 aaatatctac aatttaatta ggatattatc ttaatttgtc agataaatca cttgtgatgt
     3841 tatgtttgaa tgactagaat ttaatcagga tattatctta atttgtcaga taaatcactt
     3901 gtgatgttat gtttaaatga ctagaatttg attgggatat tatcttaatt tgt
//
LOCUS       DAGSWI010000226         3945 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.231, whole genome shotgun sequence.
ACCESSION   DAGSWI010000226 DAGSWI010000000
VERSION     DAGSWI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.231"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..382
                     /locus_tag="MJF80_003743"
     CDS             <1..382
                     /locus_tag="MJF80_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBZ0282345.1"
                     /translation="HVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNVKINLNIK
                     GYKFLPYVKNSKASRDNLCFKINTNIDEPKKYLEQNEIQIERGIVECTGVFGNMKSIY
                     LKKTNGNLIELSNHRITESKPWKI"
     gene            370..1308
                     /locus_tag="MJF80_003744"
     CDS             370..1308
                     /locus_tag="MJF80_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBZ0282346.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1692..1916)
                     /gene="secG"
                     /locus_tag="MJF80_003745"
     CDS             complement(1692..1916)
                     /gene="secG"
                     /locus_tag="MJF80_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBZ0282347.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2509..3801)
                     /gene="eno"
                     /locus_tag="MJF80_003746"
     CDS             complement(2509..3801)
                     /gene="eno"
                     /locus_tag="MJF80_003746"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBZ0282348.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1342 a          642 c          451 g         1510 t
ORIGIN      
        1 acatgtggtc attaccaata ataatataga aaattatatc aatttttata aaaaatttgg
       61 tttttcatct aaagaaatga gcaaaaatta taaacttaat gctagaaatg ttaagataaa
      121 tcttaacata aaaggttata aatttttacc ttatgttaaa aattcaaaag caagtagaga
      181 taacttatgt tttaaaataa acacaaatat tgatgagcca aaaaaatact tagaacaaaa
      241 tgagattcaa attgagcgag gaattgtaga atgtacaggt gtctttggta atatgaaatc
      301 tatttatttg aaaaaaacta atggaaattt aattgaatta tcaaatcata gaatcacgga
      361 gagtaaacct tggaaaattt aatcctatca ataaatgttg ttcttccttt gtttctcaca
      421 atgtcacttg gatatgttct aaaaaaatta ggtctatttg atactcatac tttaaataaa
      481 atgaataatg tctgcttcaa gtctttcttg ccattattat tattttttaa tatatacaat
      541 acagatttag atggttctct taattttaag cttatggctg tagctgttat aattattttt
      601 tcaatcttct taatcttagt ctttattatt cctaaaattg aaaaagataa taagacaagg
      661 ggtgcattaa tacaaggtgt atttcgtagc aatttcgtta tatttggtat tcctgtgaca
      721 atttcactat ttggcgataa gagtattgga gtcacatctt tattaattgc agttattgta
      781 cctatgttta atgtattatc tgttgtaatt cttgaaattt ttagaggtgg caaagtaaat
      841 ataaaacata tgattaaaag cgtcttaaca aatccactca taatcgcctc ttttatagga
      901 ttgacaatgc taattttaaa tattaatata cctacatttt tacacaaaac tattggtgat
      961 atttctaaaa tagctactcc tttatcactt ataattctag gtggctcttt tagtttttct
     1021 gctataaagg ggcatataaa ccccattata ttgggcgtat gtggaaaact tatacttgta
     1081 cctgctatat ttttaccaat tagcattttt cttggattta gaaatgtaga attagcttca
     1141 cttatgataa tgttatctgc tccaactgca gtttcttcat ttacaatggc acaacagatg
     1201 gattctgaca gtgaattagc tggacaaatt gtagtattta cttctggatt ttctataatt
     1261 acagtatttt taataatatt tattttaaag cagaataatc ttatttaatg atatttgata
     1321 acacaaattg gtattatgag aaacatatat aaaatcgatt taattgcgta aaaacatatt
     1381 attatactat ttttactata taatataact ttgtgtatat tatctcatta aacatggtga
     1441 aagtagtgtt gctatatatt ttttagtttg aaaaaaatat ataaatacaa acaatatata
     1501 aatacaaata ataaacgcca acaaatagtc aatctataaa ataaaaactg gactaatcat
     1561 ataaaatcat tagtccagct tttattttac gtacttttta tgttgatgta cttttattaa
     1621 aaactaaatt taatttttaa atgtctagca aatgcactaa ttaaataatg gccagttatt
     1681 caacaatatc actacttctt aactataagt aatgccaatg catttataaa aaataatact
     1741 gctgttatct ttgtaactct agcaagaaat gcttgttttc cttttggttt aaagtaagct
     1801 tgacttcctt ctccaccaaa ttctgaaggt aaagcattct tagaatcttg aggcattatg
     1861 ctaattacta gtaaaatccc tagtactact tgcacaccca ttaaaatgtt gctcattctt
     1921 gcccctccta tctgtttatt acttaaattt cattactctt tagtgtatca caataatata
     1981 gccatttcaa gattattatg ctataaataa catacaaaaa aatatattgt tactataata
     2041 tattactata ttctcaatat aaaaatgaat aaaacttaga agagaaaact atttaaatta
     2101 atacataaat taacacttaa aataatctac tctcattgta tatattataa atagccacca
     2161 aaataaaaca atcattttaa aatattgata taacttttaa tttcaacata atttaaaata
     2221 aataatccac atttagcaat aatacttcaa tgacaattat gattaaatca aaatatgtct
     2281 tcacttttat tttttaataa aaacctccta tgcaattaga taaaaatgag atagaaacat
     2341 ttattcatat tttttctgaa tctatgaata aaattaagta ataaaatata aaaaaggata
     2401 agtgaattga aaaatcaatt aacacttatc tcttttatat cttataagat gagcttaaat
     2461 atcttatatt ctctcttcat taatcttttt tagtaaccat tttaataact atttttttaa
     2521 gttatagaaa gattttaatc cacaatatct agcttgctct ccaaccattt cttctattct
     2581 taataattgg ttgtacttag caactctatc agttcttgat ggagcaccag tttttatttg
     2641 accagcattt actgcaacag ctaaatctgc tatagtagaa tcttcagttt ctcctgatct
     2701 gtgagatata acagcagtat atcctgctct cttagccatt tctatagcat ctaaagtttc
     2761 agttattgta cctatttgat taactttaac taatatagag ttagctactc cattttcaat
     2821 tcctttttct aatctctctg tgttagtaac aaataagtcg tcaccaacta attgtaattt
     2881 atttcctaat ttttcagtta ataatttcca tccatcccaa tcttcttcgt ctaaaccatc
     2941 ttctatagtt attattggga agttgtttga ccaatcttca tataaagcaa ccatttcagc
     3001 agcagttaac tcttttcctt ctcctgctaa aacgtatttt ttagtttctt tgttgtacat
     3061 ttctgtagca gcaacgtcaa gacctaacat aacgtcttca cctggcttat atccagcttt
     3121 tgttatagct tcaactatta attctaaagc ttctctgttt gaacctaagt ttggagcaaa
     3181 tccaccttcg tcacctacac cacaagctaa tcctttttct cctaaaactt tctttaacga
     3241 atggaatact tctgctccca ttcttaaacc ttctttgaaa caacaagctc ctactggtaa
     3301 tatcataaat tcttgaacat caacgttgtt atcagcatgc tctccaccgt ttaatatgtt
     3361 catcattgga acaggtaatt gtttagcatt tactccacct aggtattgga ataatggtaa
     3421 acctatttca tcagcagctg ctctagctac tgccattgaa acacctaata tagcattagc
     3481 tcctaattta cctttgtttg gagttccatc taattctatc atgattgcat ctatagctgg
     3541 ttggtcaaat gcatccattc cttctatttc aggtgctatt atttcattta cgttagcaac
     3601 agctgtttct acaccttttc ctaagtatct tcccttatct ccatctctta attcaacagc
     3661 ttcaaaagct cctgtagatg ctcctgatgg aactattgct cttcccattg ctccatcttc
     3721 taatactact tcaacttcta cagttggatt tcctcttgag tctaatactt ctctagcata
     3781 tactaattca ataactgaca tttgtatatc tcccttcgat tttctattag ttaaattttc
     3841 atttactgta aacatccgtc tactatttta atctactttc tatattatag tacataaagt
     3901 tgcttttgaa aattaggtac aaatttattt gatgggtaaa tttgt
//
LOCUS       DAGSWI010000227         3916 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.232, whole genome shotgun sequence.
ACCESSION   DAGSWI010000227 DAGSWI010000000
VERSION     DAGSWI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.232"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            163..690
                     /locus_tag="MJF80_003747"
     CDS             163..690
                     /locus_tag="MJF80_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBZ0282349.1"
                     /translation="MHEQIDCCERKKIKTINIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            674..2077
                     /locus_tag="MJF80_003748"
     CDS             674..2077
                     /locus_tag="MJF80_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBZ0282350.1"
                     /translation="MEEKYKETLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(2596..2907)
                     /locus_tag="MJF80_003749"
     CDS             complement(2596..2907)
                     /locus_tag="MJF80_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282351.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(2963..3556)
                     /locus_tag="MJF80_003750"
     CDS             complement(2963..3556)
                     /locus_tag="MJF80_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBZ0282352.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT         1483 a          449 c          612 g         1372 t
ORIGIN      
        1 aaatatgcaa aaaaattaat ataaaaggta attaaatatc aatgaagatt taaaaatagt
       61 taaatcaaag taagagtcta atatataaaa tttatagtaa ctttatggtt taaatataaa
      121 cttgtgtgct attgacaaag tataaaatta gcagtaaaat aattgcatga gcaaatagat
      181 tgttgcgaac gcaaaaaaat aaagacaata aatattaaag gagagaatat gagagagcta
      241 ttaaaatggg tttccattat aaatagatac aatacaaatt atattgacag gaaattggcg
      301 gatattggaa tcaataatag ccaatatttt tatgtattac acatatgtaa taatccaggt
      361 attacacagg acactatatt tgagaaaatt cttgtgaatc ctagtaatat aacaagagct
      421 ttggcaaatt tagaaaaaga aggctttata acaagagaac caggtgtaaa ggataaaaga
      481 acatggcatt tatatcctac agaaaaatct ttagaatgtt atgatgaaat actaaaaata
      541 acaaatgggt atgttgagga gttgttgagt tcatttgaga aagaagagca agagttgttt
      601 atgtcaatgt taaagaaagt tgcatttttg gctattgata tgaatgaaga agcaaaattg
      661 gaggcgaagg aaaatggaag aaaaatataa ggagacattg gaatatgaaa aggtttcaaa
      721 gttattaact aagtacgcaa ttcctagtat tatagcaatg ttagtaagtg ctttatataa
      781 cattgttgac caaatattta ttgggcaagg tgttggtgta ttgggtaatg ctgctacaaa
      841 tgtcactttt ccactaacaa caatatgtac tgcaattgca ttattgttag gaattggagg
      901 agcgtctaag tgttcattgg aattaggtgc taaaaattca gaaaaagcta caaaagcagc
      961 aggaaattct atatgcttga tggctgtttt tggaataagt ttatttatag tagtatcaat
     1021 atttttaaca ccactattaa aattctttgg ctctacatca gaaatactac cttatgcaca
     1081 cacatataca aaaattacat caattggatt accatttctt ataatgtcca ctggaatgag
     1141 taagttgata cttgcagatg ggagaccgaa agcttctatg ttttgtatgt taataggagc
     1201 agttattaat acaattttga atccattatt catttttgtt tttaatatgg gaattgctgg
     1261 gtctgcttta gcaactgtta ttgggcaact tatatctttt ggaataagtg ttcattatat
     1321 aaagaatttt caacatatta aattacataa gaaaagtttt aaattagata gtataattta
     1381 taaacatata tttgcgctag gttcaagctc atgttttaat caattagcaa tgactgtaac
     1441 acagatagtt atgaataata cattggcata ttatggggct aggtcagttt atggaagtga
     1501 gattccactt gcttgtgtcg gtatcattat taaagtaaat atgattttta tatctataat
     1561 tattggtata tcacaaggta tgcaaccaat tatagggtat aattatgggg ctgaaaagta
     1621 taatcgtgta aaagaagcat ataagtttgc agtgggaagt gcaactatta tatctgttat
     1681 tgcattctta tgtttccaat tcttcccaag acaaattacg tctatatttg gtaacggaaa
     1741 tgagacttat tttatatttg cagaacatta ttttagaact ttcttgttct taacatttat
     1801 aaatggtgtt caaatagtat catcaacttt ctttacatca attggtaaat cagtgttggg
     1861 actttttact tctttgactc gtcaaatttt atttttaatt ccattgatag taattttacc
     1921 aatgtttatg ggaattgatg gagtaatgtt tgcaggacca atagcagatg cagcagcggc
     1981 aatggtatgt atatactttg cagttcatga aatgaaatca ttgactataa agcaaaataa
     2041 tttgatgagt aaccataata agattgcaga ggtataagac aatatttaag aagtatagaa
     2101 aattaattta aagaagtata ataaactaat ttaggaagta tagaaaatta atctaaagaa
     2161 gtataataaa ctaatttaga aagtatagaa aattaatcta aagaagtgta ataaactaat
     2221 ttaggaagta tagaaaatta atctaaagaa gtgtaataaa ctaatttagg aagtatagaa
     2281 aattaattta aagaagtata ataaactaat ttaggaagta tagaaagcta atctaaagaa
     2341 gtataataaa ctaatttagg aagtatagaa aattaatcta aagaagtata ataaactaat
     2401 ttaggaagta tagaaaatta atttaaagaa atacaataca ttaattttta agtaacatta
     2461 aattaatgta agtaaaataa tgttcatatg aagatttaaa ggtgcatttt ctgaagctaa
     2521 gtatatgttg tagagtgtta tgtctctcta gcctgtgaag tttaatagct caagaaaatg
     2581 cactatttat gatgattatt ttgttttatt tccaattttg gaaataacta ttctaattaa
     2641 atctaaaatt ataccataaa taagaaaaga aagtatagat gctatgtacc aataaatcga
     2701 aattgaccct gtaattgttc tacatgggat ttgcatacct gaatccataa aaacaaaaaa
     2761 actatcaact ctggaaataa tagacaaaat aggatttaaa cctattagaa caatactttt
     2821 tgagtcttga ccaatttgat gcatataaat ctcaaataat gcaattaaaa aaaaccaaaa
     2881 tgtaaaagat ttaataattt ttttcatcaa aatacctcac ataatataaa ttttgtttgc
     2941 actatttgct tactacaact atttatctcc attcagtcac atcacttaat tgtaatctag
     3001 gtcttcttga tggttcttca tcaggataac ccaaagatat tgctgccact aattgactat
     3061 ctgtatttaa ccattcacat aattcacgat atgcaaagta tacatcacaa atccaaagac
     3121 tacctagacc taattccaca gctgtaagcg acatattctg aattgcagct ccaattgatt
     3181 gcatgtttgc catttcatag aatcgttctt caggggatag cttttctaaa ggtgatttac
     3241 ctaatatatt taaaataaaa attgttactg gagcttgttt catgatttca actgtgtaat
     3301 ttgcaccagc gatatgttga cgacttcctg gaagaagccc attatcatta atttcatttt
     3361 gaataccttt gctcatggct ttaagcatag attctttttc cttttcagtg atgacaacaa
     3421 aacgccatgg ttgcctgttt ttagatgaag gtgcatttat tccagcttca attattttct
     3481 ctatagtttc atgtgaaatg ctttgatttt tatattttct gatacttcgt ctttttgata
     3541 tagaatcact tatcatttta agtaacctcc gtttattttt tggaaaaatc tatttaattt
     3601 ttattttcta ttttaattat tttagcatta atcagaaaaa aagtcattga ttttagtata
     3661 caattattaa tgattatatt aagaattgta tcaaaaagta tgtgattata ctaagggatt
     3721 atattaaata ttatatttaa aattgtatga aaggttgtat ttaatattat attgagagag
     3781 atatatatta ggatttatat atattcagat ttattattat attgagcctt atattaaatt
     3841 aaggactata atttgaaaat aagctataaa aactcaatat tgttttgttc agagataaaa
     3901 aaagaatact ttaggt
//
LOCUS       DAGSWI010000228         3913 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.233, whole genome shotgun sequence.
ACCESSION   DAGSWI010000228 DAGSWI010000000
VERSION     DAGSWI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.233"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(233..433)
                     /locus_tag="MJF80_003751"
     CDS             complement(233..433)
                     /locus_tag="MJF80_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBZ0282353.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(658..834)
                     /locus_tag="MJF80_003752"
     CDS             complement(658..834)
                     /locus_tag="MJF80_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282354.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(888..977)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1157..1357)
                     /locus_tag="MJF80_003753"
     CDS             complement(1157..1357)
                     /locus_tag="MJF80_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0282355.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1359..1709)
                     /locus_tag="MJF80_003754"
     CDS             complement(1359..1709)
                     /locus_tag="MJF80_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBZ0282356.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1871..2060
                     /locus_tag="MJF80_003755"
                     /pseudo
     CDS             1871..2060
                     /locus_tag="MJF80_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2537..2707)
                     /locus_tag="MJF80_003756"
     CDS             complement(2537..2707)
                     /locus_tag="MJF80_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282357.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESGYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(3096..3590)
                     /locus_tag="MJF80_003757"
     CDS             complement(3096..3590)
                     /locus_tag="MJF80_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBZ0282358.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKSDYGSSIDIMNEQNKIDIVIKFLND
                     KSNT"
BASE COUNT         1512 a          516 c          425 g         1460 t
ORIGIN      
        1 ttgatgtgcc acaactaaaa ttattatatt actatatatt ctaattaaaa gagtacttgt
       61 aggtttaagt acatacttta taaatatatt tttgtcagat ttattttata aaaagtattt
      121 agactaagta ttatttttaa gtattttttt gttgaagtta ttttttcctt ttttacaaaa
      181 tgcgtaaata aaaaaaagac tcttaaaaga gtctttccga aaaatctata tattataata
      241 tagttatatt ttctgcttga ggacctctag cgcctttaac tatatcaaag cttacttttt
      301 gaccttcttc taatgattta aatcctgaag tttgtatagc tgagaaatga gcaaatacgt
      361 catctccact ttccattgat ataaatccaa atcctttttc attattaaac cattttacta
      421 ttccattatt cattaaaaat actcctatta acctatatca agatatactt gatttacttg
      481 tgtattacca caaatactat tgtatcatag attaacataa atgtctattt aattataatt
      541 tttagttaac atttttattt attcttaata tgaaaataaa tttaaacatt ttatattacc
      601 aatatattaa tgtaaatgta gataaaacta aaaagggttt tctagaaaaa aatctgtcta
      661 attatactta cttaccaata ccatcacttg atgcaagcaa gattccacaa ctttttctcc
      721 gtactctaat ataaaatcat ctaactgtgt atctataaaa tcacaataat catatgaact
      781 accaatataa gcagttaaca tatgtgtaca tttatccatt ttatctctaa acataatgta
      841 tttatccccc taaaaaactt tcatgaaaaa caaaaagaaa atatcttttt ggcaactggc
      901 tgacataact tattttataa gcgttagtcc ctatagcttt gcgtcactaa atttctctag
      961 ttttgccgat ttagttttat tctacagatt aataatacaa tagaattagt tattattcaa
     1021 catatctagc tgaaaaatta ataaatttat atatgaacgt taaattatct gaaaaaataa
     1081 aaagcccctc tgtcaggagt gacttaaaaa ttgaatttaa gtttgtattt aaaacacatt
     1141 tttaataatg caaaaactaa tcaaaaataa acaattcatc aataggtgta tctactattt
     1201 ttgatatatc tattgctaat ttcaaagaag gattatactt tcctgcttct aaacgcataa
     1261 tggtttctct tctaactcct actttttctg ctaattgttc ttgagttaat tcttttaatt
     1321 gtctatattt ttttaggtta caagtgaatt taggcatatt atttttcctc taaaatataa
     1381 agttttatag cataaacatt taaagtgatt gcatatgtaa ttgatagtaa aacacacatt
     1441 aattctatat tcctagacac tatagtaata caccataaaa tgaataattc tataatagca
     1501 agctgtcctg taaatgccat agctgctttt tcatcttgta catacctttc atctgcttta
     1561 tcaccattta tttttgctat aataaagttt gagaaaaaag caaaaaatcc aatatatgta
     1621 aggtcagcaa cattgccaga tgaaaaatat ctaagactta aaaatccaag gaaaccacaa
     1681 aaacccataa aaaatttttt ctgaatcatt ttttatcctc cttagtaatt ttaaaagaga
     1741 tatatttata tctttatgat acaaatatat ctcttttata ttcttttgtc aatagaaaat
     1801 cagtattata caatatttgt actgcaccat aattgttaga gcaaaaatct actgcttgag
     1861 atgtttatgt atgactaaat atgattttaa tttcaaatta aaagtagcta aattttattt
     1921 gaatagagaa ggaagagata ttttaatagt aaaacaatat ggagtttata accattctca
     1981 agttgaaaga tgggttaata tattaggaga gtgtgcacta aaaataaaaa aaattttcta
     2041 tactatatgg ttcaagttag atgttataaa ctatatgatg ataactaaat atagtgtaca
     2101 ataaatcgct aagtattttg gaataaacaa tagcacagtt atagcaacat gaaaataaaa
     2161 atttttacat agtggtgttg atgccttttc taaaatgaaa ggaagaccat aatgaataaa
     2221 aacaaaaaca ctaaagaaaa aataaattaa caagagaaca agaattagaa tatgaaaata
     2281 aatttttaaa agatgagctt gagtttttaa aaaagatgtg tgcctcaggg aagccacatt
     2341 aaaaaacagt ggtatcatta ttaatcaaaa gaaagttaga tgtattatca aaaaattaga
     2401 acttaaatgc atagcatttt ttcataaatc acataaatat aactcctata aatgtataat
     2461 tggaaaagta aaaaataata aaacaaatag aagatttaaa atatcaatag catatcagaa
     2521 aataacaaat gatatgctag aatttataaa atatatctaa tgataaaaat ggaaaatcaa
     2581 ttataaaaaa ggcatatccc gattcatttt ttgttatata gtcaaataag aatactatag
     2641 atgaaagata cgattttgca tatgtattaa gtaagtattc tgatgttttt ggttgataca
     2701 catgcaataa agaagttgtg tttgcagata aattagcaaa actatagtat aagttgcaac
     2761 aataaagaaa atgaaaatta tcatactaaa gcataaggca tgttacaatt ttattagata
     2821 ggacattaac atacattaaa gtacttttta ttataaagag tacttatttt tttgaaattc
     2881 attaatgtat aaactatcaa gaatattact caacataatt taaaaacaac ttgaataggt
     2941 atttttaaat tatgtgatat aataaaaaat agaagtgtat gccaatacac ttctgtaatt
     3001 ataactaagc attctattct tgaggagtgc ttttttcatt tctccaaaca agattgttta
     3061 tttcatcagc aattattaaa taaaatttat tgtatttaag tattcgattt atcatttaaa
     3121 aattttatta caatatctat tttgttttgc tcgttcatta tgtctattga acttccataa
     3181 tcacttttta atttatctag accacttttg cttatttctc cttctggaag tatacttaat
     3241 tctagatatt tttcatgacc aggagatgac attaatagat atgaaccatt ataaggcata
     3301 cctaaatttt ctattttatc tgctaatggg tcatctttaa agccaccatt tattattatt
     3361 tttccatctt tctttaccca tgatatcatt cctccatcag tatattttga ttcatctgca
     3421 tagtagctaa gagacgcact ttttattgca cttatatcag cttcaagttt agctatcttt
     3481 gctttttcta tatttttaaa taatgctgga agtgctacta ttgccaatat tcctattata
     3541 gcaattacta ctaataattc cactagagta aatccttttt tatttttcat ttatttcccc
     3601 tccatataaa attaataata tataaatttt tattttgttg atttctactt tctatatttt
     3661 accatatata gacaaaaaaa tcactttcta tgacaaatat actattattt tacaatttat
     3721 ggaaatgatt taaaatgaca tttcttgtta gaaaagatat aagaaatgga ttaaaataaa
     3781 gaaaactaca gtcatatcaa tgtcaataat attatttaaa agcagatgaa aataaaaaga
     3841 ggactcagaa aataatgtct atttaattat acttacttac caataccata acttgatata
     3901 aacaagatta tac
//
LOCUS       DAGSWI010000229         3893 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.234, whole genome shotgun sequence.
ACCESSION   DAGSWI010000229 DAGSWI010000000
VERSION     DAGSWI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.234"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(206..1456)
                     /locus_tag="MJF80_003758"
     CDS             complement(206..1456)
                     /locus_tag="MJF80_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBZ0282359.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1488..2429)
                     /locus_tag="MJF80_003759"
     CDS             complement(1488..2429)
                     /locus_tag="MJF80_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBZ0282360.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2669..3745)
                     /locus_tag="MJF80_003760"
     CDS             complement(2669..3745)
                     /locus_tag="MJF80_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBZ0282361.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT         1166 a          713 c          418 g         1596 t
ORIGIN      
        1 taaatataca aatacaatta tatgaatttt catcctcaat tatagcatag aatatgacta
       61 tctatatcct aaaattatcc atttctaaaa aaaaggtaac tattattaag aatatcaact
      121 tatagaattt atattccaca tacgattttc aatcaaaaaa acaagggcta aaaagtgaat
      181 tgtgcacttt ttagcccttt attaattatt catttatttc atgtaagaat agaccacatc
      241 ttactttagg ttcaaaccat gttgacttag ctggcattat cttatttgca tctgctactc
      301 ttattaaatc ttcaatacta gtaggataca ttgcaaatgc tactttcata tcttcattaa
      361 ctcttttttc taattcttca agacctctaa ttccaccgat aaaatcaatt cttttatcta
      421 ctctaggatt atcaattcct aaaatagcat caataacata gtcttgtaat atagaaatgt
      481 ctaatgagtc aacagggtct tctatttcta ataaattttt atttgctttc attttatacc
      541 acttatcacc taggtacatt ccgaaagttc cttttttgtc aggtttatat ttttcttttc
      601 cttctatttc ttctagttca aaattttctt ttattttatt tataaattct tcctcgctta
      661 aaccatttaa gtctttgata accctattat aatccaaaac cattaaattc tcatctggtg
      721 caatcattgc tatgaagtaa ttaaattctt cttttccagt ataatttgga tttttagctc
      781 tcatttcttt ggctacattt tctgctgaag ctgtcctatg atgaccatct gcaatgtata
      841 gataatctaa tttttcaaat ttatctacta ggttatctat tatatcatca ttatcaatct
      901 tccatactgt atgctctatc ttgtcatctg ttatgaaatc atataaaggt tcattattat
      961 ccataaaatc attgattata tcatctatca ccctttgatg tttgtaagtt acaagtatag
     1021 ttcccgtatt agcatcacag tattttatgt gtttagttct atcaatttcc ttttctggtc
     1081 gagtgtactc atgttttttt attgttccat tcaagctttc atcaactgat atacatgcta
     1141 ctataccaac ttgagctcta ccatccatga tttgtttata tatatatata cactctttgt
     1201 catcttttac tagaatttct tgttttcgaa actcatctaa attttctcgc gcttttaaat
     1261 aaactttttc atcatataaa tctatatttt catctaaatc aatttctgat ttatctatat
     1321 gcaaaaatga atatggattc ccttctgcaa ttcttctagc ttctttacta ttcataacat
     1381 cgtaaggaag tgctgctact ttatctacta tatatttatt tggtctaatc gccttaaatg
     1441 gtttaatcgt tgccattact aatttcctcc taattataca aaatatttta taatgctacc
     1501 cctattagat tgttgaagtc aagaaagaaa tcttgaatca catttactat ttcgtctcct
     1561 attctttctt gagcttctac tgtagatgcc ccaatatgag gtgtaggaga aactcttgga
     1621 tgatttacta agtctaaatt taatgtaggt tcttgttcat aaacatctat agcagctgct
     1681 gataatttac cttcatctaa tgcttttagt aaatcttttt catcaactaa accgcctctt
     1741 gcacagttta ttaaataagc ccctttcttc atcttattta tttctttttc agttattact
     1801 gcccccttat tcttatcaaa tggtatgtgt aaagataaga aatctgcatt ttctaaaact
     1861 tcttccatat cacagaattt ataattgtta aagccttgcg cctcacccat tatgtcagta
     1921 tatataacat tcatacctaa taattctgct ctctttgcaa cttcttttgc aattcttcca
     1981 aaacctataa gtccaagtgt ctttccatta atttctgtac ctttatactt tttcttctcc
     2041 cattttccat ctctcatagt tacatttgca gtatttatga atcttgcaag aacaaataat
     2101 tgacctattg taagctctgc aacagatata ctgcttgcat ttggagtatt cataactttt
     2161 atgccattag cttgagcata tttaacatct atattatcca gacctactcc acctcttact
     2221 atcaacttca actttttacc ctctaatgct ttatctatta catctttagt aactttagta
     2281 gctgaacgta ctacaagtac atcaacatct ttaagtttat ctcctaaaac atctttttca
     2341 taaaactgct ctatcacatt aaaatctaac tctctcaatt ttctcgctgc ttctttctct
     2401 attccatcag taactaatat attatacata acaaactcct ccaattttta ttcacttaat
     2461 ttttccaatt tttatttttt aaagtatttt catcaataaa aaactttact taatttaact
     2521 ttctgtttat ataattttca aaatattttt ttattaaagt catatcaaat ttttctattg
     2581 tttgttaaaa atttaatatg taatttttat aaaatattat ttattatata ttctaaattt
     2641 atcttaaaac atacatattt ttacaatatt atttattaaa acctaatata tcatcaatat
     2701 tatctaataa ttcttgtaca tcttcgacag tatagtcacc catatgtgaa attctaaaag
     2761 ttttttcttt taaatctcca tagccatttg ctatttggaa tcctcgttct tgtaattttg
     2821 aatttaaatt tgatacacta ataccttgag tattttctat aacagtaagt gtattggata
     2881 agtgattttc attggtaaaa atttggaagt gttttttagc ccagtttcta actaagtttg
     2941 ccatgtcttc atgtctgttg aatctgttat ctaggccttc atctaatata ttatctaatt
     3001 ggaaatctaa agcgaacatg tgagaaagag atggtgttga agggtattgg tagtttttct
     3061 tgattaagta ttcatacatt gctaacaaat caagatatac tcctctaaaa ggtacttgct
     3121 tagctctatc gattgctttt tgagaaaatg tgcaaataga cattcctggt ggtaatccca
     3181 gtgctttttg tactgatgtt atacaaatat ctattccaat cttatcaacc tcgactttta
     3241 ttccaccggc tgaacttaca gtatctacac agtagattac atcctcatac tttttaacaa
     3301 cttctccaat ttcttctatt ggatttctta ttccagttga agtttcattg tgagttacag
     3361 ttattaaatc gtattctccg gtagaaagca ccttatcaac catttctggc gttgttgcct
     3421 ctcctaattc aactttaaat atatccgctg ggacattatt agccactgcc attttgtacc
     3481 atctatcacc aaaggatcca caggaaaata ctgctgcctt tttggatgta caggaacgaa
     3541 tggagccttc cattaaacca gttccggatg acgtggataa aagtatcgtg ttgtttgtat
     3601 agaatagttt ttgcatcttt tcacttatac tcttttggag catagatgca tcttttcctc
     3661 tatgtcctat cattggtgta gccatctttt gcaagacttc ttctttaaca tcaataggac
     3721 caggaataaa taatttctta ctcattataa ttcccccctt tttagtttgt aatatggata
     3781 gcgtttttac tgtccttatt tttaataact ataatatcaa gctgaattaa ctatgtcaac
     3841 ataaaattta ttttttatat ataatttatt ttatatattt tatgaaaaca ttc
//
LOCUS       DAGSWI010000230         3882 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.235, whole genome shotgun sequence.
ACCESSION   DAGSWI010000230 DAGSWI010000000
VERSION     DAGSWI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.235"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..223
                     /locus_tag="MJF80_003761"
     CDS             <1..223
                     /locus_tag="MJF80_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBZ0282362.1"
                     /translation="DFLKKIMKFIPVAILTSLTAKDVFFNGDSLYLSIANPKIISFMI
                     VLLVAYKFKSIGISIITGVISIYLFGIIL"
     gene            complement(429..2489)
                     /locus_tag="MJF80_003762"
     CDS             complement(429..2489)
                     /locus_tag="MJF80_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBZ0282363.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMVISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(2685..3377)
                     /locus_tag="MJF80_003763"
     CDS             complement(2685..3377)
                     /locus_tag="MJF80_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282364.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
BASE COUNT         1293 a          621 c          338 g         1630 t
ORIGIN      
        1 tgattttttg aaaaaaatca tgaaatttat acctgtagca atacttactt ccttaactgc
       61 taaagatgta ttttttaatg gagatagtct atatttatct atagctaacc ctaaaattat
      121 atcttttatg attgtacttc ttgttgcata taagtttaaa tctattggta tttctattat
      181 tacaggagtg atatctatat atttatttgg aattatcttg tgatatttgg ataatgtttt
      241 attgtataag ttattttaaa actatgtgta caattttaat ttaagttgtt atattattga
      301 ttttaaataa aaaacagagt gacttaaagt tactctgttt ttactcttca ttctctactg
      361 taaatattct aatatattat aataaaagct ttcgacagta aaaaaaatat atatacttaa
      421 atactatctc attccatatt tgttatttct tcttcatatt tttgaaactc taaatttgct
      481 ttaaataaat ctccatctat ttctatataa aattttccac cttgtaaagc tactaaatct
      541 cttgcaatag caagccctaa tccacttcct tcagtattcc ttgattcatc agctctttta
      601 aatctttcca taatctcact tgggtcaaaa tttaattcat aagaagatat atttttcata
      661 gttaccttta cattttcacc atagtcatat acatcaatat agactctagt ttcttctaag
      721 gaatattttg ctatattaga taaaagattt tcaaatactc tatacattct tcttccatct
      781 gcccatatat acaccttatc atttggaaca tttaatttta aatataaatt atttttagat
      841 aatttttctt caagttcacc tatactttgt cttaatagtt gcactaaatc aatcttgcta
      901 atttgtaatt ctatattgcc actacttact ctactcgctt caaataaatc ttctatcaat
      961 aattttagtc tttttgactt tgactcaagt acatttatat aatcatttat atactctggt
     1021 tgtattgatt cttctttttt aattaaatct acataattta ttatagaagt aagtggagtt
     1081 ttcaaatcat gagatacatt tgttataagg tcagacttca ttctttcact ttttacttgc
     1141 tcttctatag ctctatcaag accttctcca atattattta tattttcagc taaagacgta
     1201 aaattatcat tcccagtaat ttttatctta taatctaatt ctcccttttt tattctttct
     1261 gtaccttcta ttacgtaact aagatatgaa agtcttttag ttatatatat tgtaaatcct
     1321 aaaaaaatac atatagaaaa cagtaatccc atacctatac ttcgagtgct aattaccatg
     1381 attaaagcta aagatattat cactataaca ataactttcc ttgctaaaga tactttcttt
     1441 atagcgttac tgacctccct tacagttttt attaaccata aaatcactct tactataata
     1501 cttgtcttaa atatacacat tttattttct aaataactca attgcacatt tataaaaaat
     1561 atattatttg atactatcag taaagataaa gaaactaatg caattacatc aatatgataa
     1621 gatacagcaa gagcatatat agttaaaatt gcaattgaaa tagatactaa tattacatta
     1681 aattccatag gtatcttact gtatattagt aaaaatacat tatttctttt taaaacttca
     1741 ccttttattt tgatatataa aattatagat aataaacata taattgctaa acttaaacta
     1801 gctacaaaaa ataatcttac tttttctgta gcttgtttaa atatttgaaa attagtatat
     1861 actattccat cttctatagt tggctcttct ggaaaagata catatgcctc aaaaccttca
     1921 tctacttcat ttagttccag actcagatta ttcagttcaa atttttctcc atttattgtt
     1981 ttaatatatg agctctttcc tatcttattt attatctcta cattacaata gctcttagtt
     2041 ttttgtttaa aatcatataa actagcatat gacgtatttg tataaaatac tcctgtgtat
     2101 gtattaataa catacacttt tatatttttt agataactta aatattcttc agaagcttgt
     2161 tcattaatca tgattttatc atactcagaa tcactactac ttaaatctat acttttacct
     2221 tcttgttgga atttaccttt ttctttacta gttaatttac cacctacaga tagcttaaac
     2281 ttgggtaata gacttgccat tgttccttcg tcaaatatat tactatcata aaaatcatca
     2341 gatatatcaa aaatcccact ctttgtagaa gttttttgta tattgtattt aggctcagca
     2401 agtcctattg tcaccaacaa tatagttaat ggaaatgaga tagatattat gattaatgaa
     2461 actaacttta atactctata tttctccaag ttgcccacct catctacatt gtcagtattt
     2521 tatataaaat aaagtttaag aaaagcataa tccccataga gtaaattaaa ctctcagaga
     2581 aaaaattaaa acatattaaa attcctaaca tttgtgtaaa tgtaaaaatg actaaaatca
     2641 aaatgcaatt aaatattaaa ctcttttctt ttataatttt tccactaaaa tttttcaatc
     2701 ttataaccaa ctccccacac cacctttaaa aatcttggtt cttttggatt tatttcaatc
     2761 ttttctctta ttcgtctgat atgtactgct actgtatttt ctgaattaaa actttcctca
     2821 ttccatactt tttcgtatat ctcatctatt gagaaaactc gtcctttatt ttctaaaaga
     2881 agttcaatta ttttatactc aattggagtc atttttatgt tttcaccatc aattttaact
     2941 tcttttgtta gtgtatttaa ctccaaagct ccacttttta gaatttcttt acatgtatct
     3001 tcataattac ctaaagttac atatcgtctt aagtttgatt taaccctagc tataagctct
     3061 aatagattaa atggctttgt tatataatca tcagctccaa tatttaatcc tattatttta
     3121 tctgtatctt cactttttgc tgaaattaaa attattggaa tatttctttc ttctcgtatt
     3181 tttacagttg ctttgattcc atctaattta ggcatcatta tatccattag tattagatga
     3241 atgtcattct ctgttattat ttccaaagct ttcattccat catatgcttt atatatttta
     3301 aatccttctg atttaagata tatttctatc gaatcaacaa tttctttatc atcgtctact
     3361 actaatatat tgtacatatt ttcacttcct ctattgtata ataatactta tctatattat
     3421 acaatatctt tcactccctt tctcaacaat gactaaattg ttatattact ttggcttaca
     3481 tttttgtaac tttcccttat aactatataa taaaatataa atcttaagaa aatattatcc
     3541 taattcttaa atatttctta agatttaaga taaattgagg tttatttgaa taaattgatt
     3601 tgaattatgt tgttttcaga ttactttcat ttaagtattt tgattgcatt cagctgaatt
     3661 aatgtatatt aattcttatt aaacagtttc tattttctag gtggaagaat ggtggaagaa
     3721 ttacatttca ctaattttct tccaccattc ttccacccaa atatacttag aaacattcat
     3781 ttcactgcaa taatagctac ttatttaaat tctattttta tacattctat ctactatata
     3841 ctgtacatat tctattaaaa tttatataac taaaatacaa ac
//
LOCUS       DAGSWI010000231         3829 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.236, whole genome shotgun sequence.
ACCESSION   DAGSWI010000231 DAGSWI010000000
VERSION     DAGSWI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.236"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..431)
                     /locus_tag="MJF80_003764"
     CDS             complement(<1..431)
                     /locus_tag="MJF80_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282365.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKL"
     gene            complement(434..631)
                     /locus_tag="MJF80_003765"
     CDS             complement(434..631)
                     /locus_tag="MJF80_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0282366.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(823..1689)
                     /locus_tag="MJF80_003766"
     CDS             complement(823..1689)
                     /locus_tag="MJF80_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBZ0282367.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(1915..2784)
                     /locus_tag="MJF80_003767"
     CDS             complement(1915..2784)
                     /locus_tag="MJF80_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBZ0282368.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(3290..3511)
                     /locus_tag="MJF80_003768"
     CDS             complement(3290..3511)
                     /locus_tag="MJF80_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282369.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
BASE COUNT         1321 a          652 c          373 g         1483 t
ORIGIN      
        1 tctaatttat tgtatacaac aacactacaa ataaattgaa ataaaataag caatccaaga
       61 acaactaaaa cccacttgct tattaccatt acagaaaata cactcaatat agaaacaata
      121 aatataagaa ttacaagaac ataggacatg atttgattag tgatacgtcc agctttatca
      181 cgtaacataa ttcttctttc atcattcatc tctatccttt gattttttat cttttcttca
      241 taatctactg ctctttcagg tttactccaa tacatatatt gaagaatcat acatacacct
      301 ggacctaaag cagctccaga taatccccaa ataaaagcct caaatgaaaa ctcagtaaat
      361 gtagccaata aaacaagtaa aattccacaa ataaaatata taattccaac atataacata
      421 ttctttttca tttttactcc tcctcataaa taaatatttc ttcaatactc atatcaaagt
      481 atctagctat cttaaaggct aagatgatag aaggattata cctaccattt tctaatgaac
      541 ctatagtttg tcttgatact tgcaaaatag aagcaagttc ttcttgtttt atgcctcttt
      601 cttttcttat ttcttctaat ctatttttca catctatccc cctttaatat ctcaactacg
      661 gaaagtctac tttccataat tatacaacta tattccattt atgtaaagct tactttccat
      721 atttacaaaa aaataacaga ctattttcag cctgtaattt tagaatttca tgttgtttat
      781 taattttaat gttatttatt aattccatat tatttattaa ttttaacttg gtttctgaat
      841 tggaatccaa acttctgtag tataatcagg agcattgata tcgccattgg gataaagttc
      901 aatatctggt aaattagcat acacataccc agaacttggt aaccactcag taataattct
      961 tttttgcaat tcctgtattg ccagagcatt tggcattgga gaaacacatt caaatactgc
     1021 ccatgtgcct gatggtatta agtattcatg agtatcttct aatacaggct tatctgttgg
     1081 agcagcaata taataatcta aacccttact atttgtacac acactaactc caagcaatcc
     1141 acaaggttca ttatttatta actctgaaag tttatcaatt gttccatttt ttatgcattt
     1201 agcccacaac ttaggaactt tctcaaagca ctcttctaca atcatgttca tactttcttt
     1261 tataccaaca atacgaaacc ctttcttttc tacaatacga tattgcattt ctatatttcc
     1321 ttttacataa atgcttaatg tcatcttagg atatgctttt aaaacaacac cctttgctct
     1381 agctgcagat ggagaaatgt tatgtatatt ttgaaaagct ctattaaaag atgttggtga
     1441 atcgtaacca tacttctcag atagttctaa aattttgatg ttactgttct gaagttcaaa
     1501 tgcagcctta gtcatacgtc ttcgtctaat atattcagat aatggtactt ctgcaatata
     1561 tgaaaacatt ctttgaaaat gaaaggttga acaacatgca atttttgctg cttctgcata
     1621 atccactcca ctttcgagat tttcttcaat ataagtaata gcttgattta atttatttat
     1681 ccattccatt tgatattacc tccaagcaaa ttatacagtt taataaaata atatacctct
     1741 ctattcatgc acaaaattgt aagcatttaa attcataagt atttttcaat caatacaata
     1801 aatataaaaa ttgtattata caataaaaaa gttcaataac attatattat aaaatgttat
     1861 tgaacttttt ataaaaatta atttaagtgt agttaaatta agtattacac tatcttatat
     1921 attacaatat cctactatct tatagtcttc tggcacttta taatctttgt ctggagtacc
     1981 taaattccat tttaaatcaa acatagttgc acttattatt gttgcaaaat aaagcataac
     2041 tataccaatg tctatttttt cttcatataa attagtatga ccatcttttc taacagctaa
     2101 aactacagtg cctccatcaa ctataaatct ccatggttgt ctatttaatg tagatggagc
     2161 taatcttgca tagtgaaatg catcaagaag tgctctttct tctaagattt ctatacttgc
     2221 aacatttccc cattcgttca tataaacaac atcttctaca cctagtctag tagacccctt
     2281 atctgttaca acttgcatat cagattttga ataattttgc ccagtttttg tagcatttaa
     2341 tactttagca ttacttacat tttctccata tccaatagct ataattcctg ttacctcttt
     2401 atctgaatta attccaagtt tttcttttac cttttttcca tcttcaaaag taacccaaca
     2461 agaaccaatt ccaagttctg ttactttaaa gattatactt tctcctatgt accctgaatt
     2521 ttcaatgtaa ttatctttta tgtcagataa tattataata taacttggag aatctatcat
     2581 atttcctttg taccctgcta ctttatctaa attattaaaa acttcacttc tattcataat
     2641 tcttacatca acatctattt ctggaactag tttcttacaa gattctgagt atttcttaat
     2701 ttcactaaga tattcttctt taatatctgt gttcttatat tctctaacag actttctatt
     2761 taatatgatt gttttataat ccataatttt atcctcccca aactagtatt gtcttaatta
     2821 tatacccatt aatatattat tttactcatt tatttaataa ataaagatta caattttgta
     2881 attattattt attaataata ttccactttt acattaataa tgaccagttg tgcaaaatcc
     2941 attgccaacc accaaataaa aacatattat aaaggtaata taaaataaca aacaaatctt
     3001 aacaaaaaat taaacttttt ttacacgaaa gaaggcttcc cgttgccttc tttattttta
     3061 taaaaaaata agctgtttct aaaatgattt cacatttttg aaacagccaa gttattctat
     3121 aatgacaact ataatttgag gatgtgaggg agagttgtca ttataaattt agggtaatta
     3181 tattatgttt taagctagtt ctttctatat ttatataata cattttatat atgaatttta
     3241 tatgagattg gtataaatga tatatgaaat tacatatgtt tatattatat taaattttat
     3301 tattacattt tccacaacaa tactcatatg tctccacatt atttggaata tttacaaata
     3361 caaattcttt acagttctca caatacttta atatagatat agttccacaa gtgtaacatt
     3421 caaatattac acaatcctca ttttcctcta ctatttcaaa ctttttacta tgttcaccac
     3481 actctgggca cttgtgatta tttacattca taaatactcc tccttattag tatagttaca
     3541 tgtcaaaaac ttaattaatg tattctatat tacttattat atattctata ataaatcata
     3601 ttctaatata ataaaacaaa ttaaataaaa aaaaaccata aattttaaat ttatggatta
     3661 attcttgtaa tgataactct atttgaggat gtgagggagg gttatcatta caatagggta
     3721 ttatattatg gtttttaagc tagtctttct acacctcata tgatacatat gctatatgaa
     3781 ttttatatga tttttacata aatattttat gaaatttaaa ttttttagt
//
LOCUS       DAGSWI010000232         3811 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.237, whole genome shotgun sequence.
ACCESSION   DAGSWI010000232 DAGSWI010000000
VERSION     DAGSWI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.237"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            289..2199
                     /locus_tag="MJF80_003769"
     CDS             289..2199
                     /locus_tag="MJF80_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBZ0282370.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEEYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(2262..2885)
                     /gene="ytaF"
                     /locus_tag="MJF80_003770"
     CDS             complement(2262..2885)
                     /gene="ytaF"
                     /locus_tag="MJF80_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBZ0282371.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            3073..3717
                     /locus_tag="MJF80_003771"
     CDS             3073..3717
                     /locus_tag="MJF80_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0282372.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
BASE COUNT         1655 a          381 c          566 g         1209 t
ORIGIN      
        1 tcactgtaca aaaacattaa tgacattatt tttattgaca taaatatata atttatggaa
       61 taatattaga tgcactgata taagtgttta aataagaata gaaaaggaga agtgttttat
      121 gaaaaaaaat actaacatta atgagtccgg agttaaactt cttaataaat aaggagtttg
      181 atatataata actccttatt tattaagttg ttcgcaaaat aactattatc aaagtaaatg
      241 atagggtgat ttaaaagacg ataacttata aataaaggag aaaaaataat gaatagtaat
      301 acatttgaaa gattacaact aaatgaagtt aaagaattaa taaaaataca ttgtgtaagt
      361 tcactaggaa aaaatttaat agataaatta actcctagtg ggaatattgg agtagtaaga
      421 aggaaattag cagaaaataa agaagctaga aagataattg aaaatagtaa tcacatacca
      481 cttgaaggat tgtttaatgc aggttctaca atagataaaa ttgaaaaagg aatgataata
      541 gaacctgttg aacttgtaaa tattgaagat tttttaagag gttgtaggaa gatgaaagct
      601 tttatgttag aaaaagaatt ttattcacct acattaagtt cttatgcctt aaatataaca
      661 gaatgtaaaa gtatagaaga tgagataaat tactgtataa aatctaataa agttgattct
      721 aatgctagta aagaattaaa aaagataaga cgaaatatag aaattacaga aggaaagata
      781 aaagatagat taaataaatt tataacatca actgtaaata aaaagtatat acaagagttt
      841 ataataagta aaagaaatga tcgatatgta ataccaatta aatcttctta taaaaatgaa
      901 gttaatggta ctatattaga tacatcatca aaaggaaata ctgtatttat tgaacctatt
      961 agtgtttcaa atttaagtac agagttaact atgctaaaag ctgatgaaac tattgaagaa
     1021 tacaaaatat tatcttattt aacagaactt atatttgaga agataagtca gataaaatta
     1081 aatatagaga tactttcaga atacgatatg gtatttgcaa aagctaagta tagtcaaaaa
     1141 ataaaaggaa ttactccaaa aataaataat aatggctata taaaaataat taaaggtaaa
     1201 catccacttc taacaggaga tgcagtacct ttagattttg aaataggcaa aaattataga
     1261 agtcttataa taacaggtcc taatgcagga ggaaaaactg taacccttaa gactgttggt
     1321 ttactaactt taatggttca atgtggattt gatatttctg caaaagaagg tagtgaattt
     1381 agtgtatttg aaaaagtttt tgtagatata ggggataatc aaagtattga aaatgcctta
     1441 agtacttttt catcacatat aaaaaatatt gcagagatta tgagtttatc aaataattct
     1501 actcttgttc tgtttgatga aataggaagt ggaactgaac ctaatgaagg agctggttta
     1561 gcaatatcat tattagaaga gttttacaaa atgggatgta taactatagc atcaactcat
     1621 tatggagaga taaaaaagtt tgctacattg catccagaat ttgaaaatgc tggtatgatg
     1681 tttgataaag aaacgttaga accattgtat aagttaacta ttggaaaatc tgaagatagt
     1741 aatgctcttt ttatttctaa aaaaatggga ataaaaaata gagttttaga tagagctaaa
     1801 gaatatgtaa ttgatagaaa ctacaattta gatttagtta aaagaagtaa gctacaatct
     1861 aatattgaag ataaaaatag ttctgaagtc attttaaact atacagatta taatgtaggg
     1921 gataaggtaa aattactaga tgaagatgat tttggaattg tatataaacc tatggacaag
     1981 tttaataatg tagaagtatt gtttaatgat aattttattt cagtaaatat aaaaagatta
     2041 caacttagca taaaagcatc agaattgtat ccagaagagt atgatttaga tgtattattt
     2101 actagtttta aagaaagaaa attagaaaaa gatattcaaa gaggttcaaa aaaagctctt
     2161 aaaaagattc agaaagaaat tagagagaat agaaaataaa tataaaaagc acaaaagaaa
     2221 gcaatctaaa gagcaaagtt tatctttaga ttgctttctt attaacatat cttcataaat
     2281 ccaatgacta acaagattaa accacttaac caagataagt ttaaatttac gtttttaaca
     2341 aacatatttc ctagtaaaca accaagtaat actgcaatta tatttgatat taaagaaaat
     2401 agaataactt gtatataatt cacacctaca aggccactac caaaaccgat agctaatcca
     2461 tctaaactta aagcaagtgc aagtgtaaat gcttcagcag atttaagcac tttagattta
     2521 cttgtatcta agacatcagg ttcgatactt acattaaaat ttaatttaag gtctaacata
     2581 ttaaaattat aattctttga gtgtatagat tttttcttaa aataagattt aaaaaaactt
     2641 tcaattagtc tagcacttcc taataaaaat aaaataaaga aacatattaa cattgttata
     2701 ttttgaggta aaaagtctct aactattgta cctgcaaaaa gcgatattgc taaaatagct
     2761 gaacaaacta cgctgataat tatatttgaa tataagggaa ctttaacttt atttacgcca
     2821 tatccaaagc ttgtcacaaa ggcatctata caaagagaag taactaataa aattgattca
     2881 atcacagtag attaacatcc tttctcttaa attttgtact tatattagtt atagtattgt
     2941 aaaaatggat aaattgttac agataatcca ataaattgtt gataaaattc caacatcata
     3001 caacacctaa ataacaaaag aatcatatca tataataata taaaaccaat cataataaag
     3061 cgggtgaaaa taatggaaca aaataataag gttgggaatt tcggtgattt gttgaagatt
     3121 ttattagatg aaaaagaatt atcaatggga gaactaagta aaaaaagtgg aattgataaa
     3181 tctactatat ctcgtatagc taataataaa caaaagccaa atataaatca tttagaaaag
     3241 atggcaatac atttaaatat taacctagaa gaactgttga aagcttcagg atatgaattt
     3301 agaaacagca ataatcaaat atttaatgca gatagtgatt ttagtaactt tgatgatatt
     3361 cttggatttg caaatttaat caatgataag aactttaatg gaaatattga aaaagagttg
     3421 agtaagtgta agttatatgt ccaaacagat gaaggtaaaa aattactttt tgataatttt
     3481 aataaaaaaa tagattctat tgaaaaacaa ggtcaattta cagacagatt aagacaaatg
     3541 tatgctgatt tttgtacaga tggacttaca ataaagaaat atttattgat tggaagtatg
     3601 ctactttact ttgttatatc aacagatgtg attccagact tcgtgttccc aataggattt
     3661 atggatgatt tagtagcatt aaatatagtt acaaaattac ttaaaaatga taagtaagtt
     3721 ttagaatcat atttaatata taaataaatc aaagtatata aaatttatta ttttttatat
     3781 gaaacaaatt aaaagcacaa tgaagtagat t
//
LOCUS       DAGSWI010000233         3803 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.238, whole genome shotgun sequence.
ACCESSION   DAGSWI010000233 DAGSWI010000000
VERSION     DAGSWI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.238"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            228..1562
                     /locus_tag="MJF80_003772"
     CDS             228..1562
                     /locus_tag="MJF80_003772"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBZ0282373.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPSIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNISK"
     gene            complement(1704..2621)
                     /locus_tag="MJF80_003773"
     CDS             complement(1704..2621)
                     /locus_tag="MJF80_003773"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBZ0282374.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            2829..3713
                     /locus_tag="MJF80_003774"
     CDS             2829..3713
                     /locus_tag="MJF80_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBZ0282375.1"
                     /translation="MKVLKKITILLLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
BASE COUNT         1479 a          411 c          668 g         1245 t
ORIGIN      
        1 ttttgaaaaa tgtgattaag tacagaaaaa tagggtataa taaatgttta aagaatttga
       61 attgtggtaa gaaaataaaa aatagctttt ataagatttg atttcaaatt aaaatgtgtt
      121 aaataaataa ctaaaaatta ttataaaaaa tgttttcata gaatatatat caaatgaaaa
      181 gcttaaaata agtgaagaaa ttaactagac atcaaaggag agataaaatg agagtaataa
      241 taattggtgg tgttgcagct ggtatgagtg ctgcagcaaa attaaaaaga ataaaacctg
      301 agtatgaagt tgttgtttat gaaaaaactg aaatagtatc ttttggtgct tgtggattac
      361 catactttgt tggtggattt tttaatgatg ctgatgaatt attagcaaga acaccagaga
      421 aacttagaga agctggaatt gatttaaata tatttagaga agtcgttgag gttgatagtg
      481 aatctaagaa aataaaagta aaaaacataa aaacagatga aatatatgaa gattactatg
      541 ataaattgat gatagcaact ggagcaagaa gtattatgcc ttctataaaa aacataaaat
      601 tgaagaatgt ttctacatta aaaagtttat atgatggaga atatctaaaa aaacttttaa
      661 gtaatgaaga taataaaaga gtgacaataa taggagctgg ttttataggg ttagaagcag
      721 ttgaggcatg taagaaatta ggtaaagatg tagatgtaat tcagttagaa gatagaatat
      781 tgccacaagt atttgataag gagatgacgg atgtattaga agaagaaata aatagacata
      841 atgttaattt gcacttagat gaaatggttg tagaactttg tggagaagat aaagtagaaa
      901 aagttataac aaacaaaggg gaaatagata cagatgtagt aataattgct actggcgtga
      961 gacctaatac cgagtttttg tcaaatacta atataaaaat gctaaaaaat ggagctatag
     1021 tagttgatga gtatggaaga acttctgtag aagatatata ctctgctgga gattgtgcaa
     1081 ctataaagaa tatagtaagt aatgaaaatg tatatgttcc tttagctact ggagctaata
     1141 aattgggtag gatagtagga gaaaacctag caggaagaga agtatcttat caaggttcac
     1201 tatcttcaag ctgtataaaa attatggata tggaggcagc atcaactgga attacagaaa
     1261 ggcaagctaa agatttaggc ataaatgtca aatctaaatt tatttctgat tataatcaaa
     1321 ctcattatta tccaggaaga aataaaatat atgtaaagct tatttatgat gcagatacaa
     1381 aagttatttt aggcggacaa gtggctgggt ttaaggatgc tgtccaaaga gcaaatgtat
     1441 tggcagctgc tatctttgga aaaatgacca caagtcaact tggaatgtta gatttatgtt
     1501 atgctccacc atttgcaaga acatgggatg ttttaaatgt agctggaaat atatctaaat
     1561 aatatttaga ttatagctaa tatagttgac aagcaattaa gggaatgaat ttacctaaac
     1621 tatttttatc atggtaaaat tcattcctaa atttttattc caaattttta ttccaaattt
     1681 ttagaaataa ggattccatt aaattattca tcaaaattta aacgcaagct taatctatct
     1741 tctccaagaa tagtattttc aaaaacatta gtagcatttt ctaaatacat actaggtatg
     1801 tctaaagaag gactaaaatg agtcagtaaa agttgtttga catttccaag ttttgcaaga
     1861 ttagcagctt ctctaaaagt catatgtttg tttctaacag cttttgatat atctaaatca
     1921 tcaccataca tggcttcaca tataaataaa tcactatctt ttatgaaatc tggtattgat
     1981 tctataggtc ttgtatcggt tgtaagactt atcttaactc cctttcttaa ttctcctaag
     2041 accatatctg gagtgtattg ttttgaatct aaaactatat tttgaccttc ttggagtttg
     2101 ttccataata ttttaggaac ttcgttttta gttgccttat ctatatcaaa tttagggttt
     2161 cttctaaaat taaaactgta tcctaaacac tctatagagt gttctaaact gattgttgaa
     2221 atttctaaat ttcttaaaac tttattatct aaagaaaaat tgccttgagg gttttctata
     2281 atttttaaag tgtatggtac atattctact aaatttctca tagaacgtat acaatcgact
     2341 attccaactg gaccaactat agttaaatca cttgtacgac cactattccc aattgttgat
     2401 aaaagtccta aaagtccaaa tatatggtca ccatgtaaat gggttataca aatcaagtct
     2461 atatctttaa agccacattt ttttagcttc atagatacct gagtaccttc accacagtca
     2521 attagtattt ttctaccttt atagtttata aatacagagg atagaaatct gtttggcata
     2581 ggtacatttc caccacatcc aagtaaagtt aaatctatca ttttttcccc tttctaaaat
     2641 taacacaaat ctgttgtttg tttttattat gatatcctat tataattatt ttaacattaa
     2701 atataacaat aaatgtgtat ttgagtcaag aaatattgtc aaataaagtt tttatataac
     2761 aatgtataaa aatttggagg acaaaaagtg aaaattatat ttagaagtat ttaaaggagg
     2821 aaaatttaat gaaagtatta aagaaaatta ctattttgtt attgattaca ttaactttag
     2881 ttggttgtag taacggagaa gagaaaaaag tggttgaagg ttctaagaaa actcaaaatt
     2941 ctaaaaaaga tgaacagcct ataatagtgc ataaagatgc aaatatattg aaagataggt
     3001 atgagccacc aaaaggattt aaaagagttg atgtagaaaa aggtagtttt gaatcatttc
     3061 ttaggaatac taagctaaaa aattatggag agaaagtaaa gtattatgat ggaagaacta
     3121 aggaatcaaa aggtatttat gatagtgtat ttgatgttga tattggaaat agagatttac
     3181 atcaatgtgc agatgcaatt atgcttatga gagcagaata cttgtatcag aataaactat
     3241 atgatgaaat aagttttgat tttgttgatg gttttaaggc gaagtattca aaatgggcac
     3301 aaggatatag aatttcagta aaagatagtg gttcaagctg gtataaagca actgaatatt
     3361 caacatctta tgagagtttc agaaagttta tggatatagt gtttgcatat tcaggaacgt
     3421 tatctcttga aaaagaattg gagcctgtaa aagttgagga tatgaaaata ggagatgtgt
     3481 ttatagttgg tggaagtcca ggacatgcaa gtataattat ggatatggca gaaaatgaaa
     3541 agactggtga aaaggtattt atgatagccc aaagctatat gcctgcacaa caaacgcaac
     3601 ttcttattaa tcgtaaaaat ccaaatttga gtccttggta tagtttagat tttgaaggtg
     3661 atttgacaat acctcaatgg acttttaaaa gagaccagtt gaaaagattt taaaataata
     3721 attttatata ataaaagaaa aatatagatg tgaaaaaatg taattagatt aaaactaatt
     3781 ggatatggac aaaattaaat tga
//
LOCUS       DAGSWI010000234         3787 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.239, whole genome shotgun sequence.
ACCESSION   DAGSWI010000234 DAGSWI010000000
VERSION     DAGSWI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.239"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..920
                     /locus_tag="MJF80_003775"
     CDS             <1..920
                     /locus_tag="MJF80_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBZ0282376.1"
                     /translation="GFIYWYSSKKFNIKVMNFDKYIAILMIPLLLIILFMQSFEYSSN
                     INIDTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQALEDDKERAIM
                     NQQIHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKAQDYLNKLEKVT
                     SDLSFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDFDLCIVFANAID
                     NAIKACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGLLNIKEIAKK
                     YQGAISIDKTSDYFQINVLLVISLHCASI"
     gene            959..1444
                     /locus_tag="MJF80_003776"
     CDS             959..1444
                     /locus_tag="MJF80_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282377.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(1707..1943)
                     /locus_tag="MJF80_003777"
     CDS             complement(1707..1943)
                     /locus_tag="MJF80_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282378.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTYKIQTAS"
     gene            complement(2099..2323)
                     /locus_tag="MJF80_003778"
     CDS             complement(2099..2323)
                     /locus_tag="MJF80_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBZ0282379.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2420..3673)
                     /locus_tag="MJF80_003779"
     CDS             complement(2420..3673)
                     /locus_tag="MJF80_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBZ0282380.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
BASE COUNT         1503 a          487 c          491 g         1306 t
ORIGIN      
        1 ctggatttat atattggtat agttctaaaa agtttaatat taaggtcatg aactttgata
       61 aatatatagc aattttgatg attccattgt tgcttataat attatttatg caatcatttg
      121 aatatagttc aaatataaat atagatacaa gtcttgggat tgtaaagctg ctattaaata
      181 cttctataac tgaagaaatt caagcttttc ttttttctat aattggaact atatgtgtat
      241 ttttttctct atttaccttt aagaaactaa ttcaagctct cgaagatgac aaggagaggg
      301 caattatgaa tcaacagatt catgctcaaa aaaactatat tgaagaagcg aaatcacgat
      361 tatcacaaac tatatccttt agacatgatt ttaataatca tttagctatt gtcaatggat
      421 tgttgaaaaa agaccaaatt ttaaaagcac aagattattt aaataagctt gaaaaagtaa
      481 caagtgacct atcattttct tgtaatacac agaatattgt tattgatgct cttttcaaca
      541 ataaattaag tatagcaaaa caattaggca tagaggtaga ttgtgaagta attatacccc
      601 aaagcactaa catgaacgat tttgacctat gtatagtgtt tgctaatgct attgacaatg
      661 caattaaagc ttgtagagtt gtagataaac aaaataggta tttaaagctt tccactaagt
      721 tagagggtgg attttttatg atagaaataa aaaatagcta taatccaata aataattata
      781 caagtggctc aggtataggt cttttaaata taaaagaaat tgctaaaaaa tatcaagggg
      841 ctataagtat agataagaca tcagactatt ttcaaataaa tgtattgcta gttatttcat
      901 tacattgcgc tagtatttga gaacaatttc attgaaaata gtatttgtaa gctatattat
      961 gtacttgaat agagaaagtt tgatatatag aaatgaggag gttactaaaa tgaaagttgg
     1021 aaatataaat ggtcaagcac caattacaaa tggtttagat agttgtcaaa gtgaggtaca
     1081 aggcttagaa caaaagaaaa aagcattaca ggatgaaata gagaaagtaa gaagcgataa
     1141 aagtattgat ggtgaaacgg ccaaattaaa aatagaagct ttaaaaaaag aaatggaaga
     1201 gattgaaaat aaaattcaag agttaaaagg aaataaagga aaaaaaacaa ataaagaaga
     1261 aaataacgtt tcagaagtaa ataataatct attagagaga aaaaaagaac cttctaataa
     1321 tgaaaatgta aaacaattat atgatgtttt tgaaagaagc aatgtagacg aacaatggga
     1381 taatacttat cgtgttctcc aagatgataa aaacttaaaa gtaaaattta atagacctat
     1441 atagtttatt tataaaaata gttaatattt taattatcac cactttttag aagaatacac
     1501 actttatttt aaagacccac atatttattt acagttagca catttatatg tggttttata
     1561 actattattt acaagtctat attgataact tttacatagt atataatgtt taaatactta
     1621 ttcaaattta tctatacata aacaaaaaaa gagattttaa aatctctttt tagattgttg
     1681 acttatggct ttttgattta ataaaatcaa gaagccgttt gaattttata tgttggctga
     1741 tacccttctg atattgaaac accatttttg tttccaaaat cgtttaaaat taagaactta
     1801 ttatgaatga ctatttcatt atgaacaata agcaaataac atgaatcttc aatcaatgtt
     1861 ttaacagtag cattaaatgc ttgcttatat agttctatcg tttgtttgaa ctgcccatta
     1921 aagtaaatat gagtcaataa cataactata tctacacaaa ttaatataaa ttttttatac
     1981 tgtacattat ctaactgttg attatctgta ttacaacatg attgaattta aaaagccatc
     2041 aacacataga atgttgatgg cctgaaaata gattttaaaa tctctttttt attatatatt
     2101 aattattttt taaatttata ttttttgcat cagattttct aatagctaag tcaaaccctc
     2161 taaatctaat cactataggg tcattaagag gagctatctt tttcacttca acttcagtgc
     2221 catctattag tcctaaggct agaagtctct tcataagttt ttcatttcca gctatattgt
     2281 ctataattcc cttttgtcca agttttaagt tgtaaacagt cattattaac acctcctaat
     2341 gaaaatcatt atcaaacttt atatttataa tatataacaa aaagagcaaa ttgtcaatat
     2401 ataacaaaaa ataaacatac taagttagta tgtttataaa aaatagtata catgggatac
     2461 taatagcatc tagcaaaaaa ccacttacta atggtacaac taaaaaggct tttggagaat
     2521 caccatattt ttgtgtcaaa gaactcatat ttgccagagc gttcggagta gctcctattc
     2581 catgtccaat caaaccactt attatgatgg cagaatcaaa atcttttcca agtactctaa
     2641 agcaaataaa tattgaaaat aggattataa agattacttg aaaagttact attaagagaa
     2701 taggaggtaa taatgtagat aattcaaata aatctatatt cataagtgat aaagttaaaa
     2761 aaattcccaa tgaaagttct tttaaaaaat ctataagttt aaaatttaat tctagtatat
     2821 ttatcttatc atttaaattt ctaaaaataa cagccgaaaa catacaagtt acaacagagg
     2881 gtaatagtat attaaacgtt atatagaagc atctacttat aatttctcct gtatttatac
     2941 aaataaatat gagaagaacc tgttccaaaa atacagatgg tgttattgtt tcggagaagt
     3001 tattattttt tatcctattc ttatttctaa aaaatttact tccaaatctc tttagatttc
     3061 tattatatct aggaatcgac aagtctatat ttgatgatgg ttttaattta tatttattta
     3121 ttagaaattt agctacagga catcccaata taccacccat aataagtcct attgttgcag
     3181 cagataatcc aaccccaact gctccttcta taccgagatt ttctattgta agtccaaatg
     3241 cagcagaata accatgacca ccttccatag atacatttcc acacataagc cctaaaagag
     3301 gttcaagttt tataatcttt gatattgtaa tagctaatat attttgacaa aatgccaata
     3361 ctccacacaa aatccaatat ctaaataata cactaccacc tttctttata agatttatgc
     3421 ttatttcaag tcctatagtt gtaaaaaaga aggttatgaa atttggcatt aaatttccac
     3481 ttatagatat atctgctata ttaaaatatt taaaaaataa atttagaaaa caaaataata
     3541 gacctcctat tactggtgca ggtatacaga aattatttaa aaagttaact ttattcttca
     3601 ataaattacc aattaaaaag aagaatatag atagtgtcaa ggtttgcact atatcaaaac
     3661 ttaaggtaat caaaataagt cctcctttgt aattcaaagt tttaattata taacttaaat
     3721 tcttatgtat tgaaatttta ttatcaaatg tatattaata aatgtatatt aaaaagaatc
     3781 agtagat
//
LOCUS       DAGSWI010000235         3608 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.240, whole genome shotgun sequence.
ACCESSION   DAGSWI010000235 DAGSWI010000000
VERSION     DAGSWI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.240"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            152..976
                     /locus_tag="MJF80_003780"
     CDS             152..976
                     /locus_tag="MJF80_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBZ0282381.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNMNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            1105..2370
                     /gene="gluD"
                     /locus_tag="MJF80_003781"
     CDS             1105..2370
                     /gene="gluD"
                     /locus_tag="MJF80_003781"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBZ0282382.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            2583..3173
                     /locus_tag="MJF80_003782"
     CDS             2583..3173
                     /locus_tag="MJF80_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBZ0282383.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRGTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LMALIISLFSSITFFKFDSTSIIEENDFLERLRYTFEGRQEDMFVTIDFTYADAPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
BASE COUNT         1448 a          340 c          650 g         1170 t
ORIGIN      
        1 ataaagaaat ttcatgttgt agtatatttt ttgcttataa attttggtac tggatataat
       61 attaaactgt taatgatata ataaatttaa tctataaagt aaataaaatt ttgtaataaa
      121 tagttaaagc tattttataa ggagatatat gatggaagta tatgctttgg ttgggtcaag
      181 tggcacaggg aagagcttta aggctttaga gttcgcatat gaaaatgata tagaatatat
      241 aatcgatgat gggattttaa tatacaaaaa caaggtttta gctgggatat cggcaaaaca
      301 agcaaatact actatagaag cagttaaaag ggctattttt tataatttgg agcatagaca
      361 agaagttaga gaagcaatta aaaaagaaaa tataagaaaa attcttatta ttgggacatc
      421 aaaaaaaatg gttaatcaaa ttgtagagag attgtcaatt ggaagggttt gcaagtttat
      481 aaatatacaa gatataagta ctaaatatga aatagaaata gcaaaacaag ctagaaaaga
      541 aggaaatcat gtaattcctg ttccagcagt tgagataaaa agtatggcaa gtggtcttag
      601 tataaattct ttgaagcgac tttttagaaa aggaaataat atgaatatgg tagttttaga
      661 aaaaacaatt ataagaccaa cttttagtta tataggaaag ttttatatta gtgctgatgt
      721 aattaaacag attattgaat atgaagtata caactttgga agcattgata gaataaataa
      781 aatcaatata gagaattgca ataattttat gaatattttt gtaagtataa atatcaatga
      841 tttagaaact ataaaaaaaa ttgaagacat acaacagaca ataaaaaagt ctattgagaa
      901 aatgacttta gttaatgtac aaaaaataga tattaacatt cataaaatta aaaaaataaa
      961 cttcctccaa tgttaaaaaa taaactgaga aaatgatata ctaatttgca ataaagtgtg
     1021 ctataattaa actgtaaatt taggaaatgg ttttttctaa atggagtata cataattata
     1081 acgtatttat aagggggctt tcaaatgtca ggaaaagatg taaatgtctt cgagatggcg
     1141 caatctcaag taaaaaatgc atgtgataaa ttaggtatgg aaccagcagt ttatgaatta
     1201 ttaaaagaac ctatgagagt tatagaggtt tcaattccag taaaaatgga tgatggttct
     1261 ataaaaactt ttaaaggatt tagatcacaa cataatgatg cagtagggcc aacaaaaggt
     1321 ggaataagat tccatcaaaa tgtttcaaga gacgaagtaa aagctttatc tatatggatg
     1381 actttcaagt gttctgtaac aggtatacca tatggtggag gtaaaggtgg aataatagta
     1441 gatccatcaa ctttatctca aggtgaatta gaaagattaa gtagaggata catagatgga
     1501 atatataaat taataggtga gaaagttgac gttcctgcac cagacgtaaa tactaatggt
     1561 caaataatgt cttggatggt tgatgagtac aataaattaa ctggacaaag ttctataggt
     1621 gttataactg gtaagccagt tgaatttggt ggttctttag gaagaacagc tgctactgga
     1681 tttggtgttg ctgttactgc aagagaagct gctgctaaat taggaataga tatgaaaaaa
     1741 gctaaaatag ctgttcaagg tataggaaac gttggttctt atacagttct taactgtgaa
     1801 aaacttggtg gtactgttgt agctatggct gaatggtgta aatcagaagg ttcttatgct
     1861 atatacaatg aaaatggttt agatggtcaa gctatgttag attatatgaa agaacatggt
     1921 aacttattaa acttcccagg agctaagaga atatctttag aagagttctg ggcttcagat
     1981 gttgatatag taataccagc tgcattagaa aactctataa ctaaagaagt tgctgaatct
     2041 ataaaagcta aattagtttg tgaggctgct aatggaccaa ctactccaga ggctgatgaa
     2101 gtatttgctg aaagaggaat agttcttact ccagatatat taactaatgc tggtggagtt
     2161 acagtttctt acttcgagtg ggtacaaaac ttatatggat actactggtc agaagaagaa
     2221 gtagagcaaa aagaagaaat agctatggtt aaagcatttg aatctatttg gaaaatcaaa
     2281 gaagagtaca atgttacaat gagagaagct gcttacatgc attcaattaa gaaagttgct
     2341 gaagctatga aattaagagg atggtactaa gatttactct taataaataa taggatataa
     2401 ttatttaaga tataaagggg ctgtacatag tacagccctt aaattatgtg gcttgcaatg
     2461 aagtaagcta tttttgtttt aacttataaa taacttgata aattatgtaa tttagaatgt
     2521 attttttacc accacatgat tacaaattag aagagttaga ttagattttt agacatgaac
     2581 ttatgcattt taaaagcaaa gaccttaatt taaagtatct agtattattt ttaaaaatag
     2641 tatattggtt taatccattt atatatataa tggaaaaata tatggatttg gattgtgaac
     2701 tttattgtga tgagagagtt ttaaaaaatt gtactgtaca acaaaagcaa gaatatgctc
     2761 taactattgt aaattcaatg aaaagaggta caaatttttc aaataaattt gtagctggat
     2821 taaataaaca atctgatatt agaaaaagag taataaacat gttcagtgaa aaacataaaa
     2881 atggtatctt aatggctttg attataagct tgttttcttc tattacattt tttaaattcg
     2941 attcaactag tattattgaa gagaatgatt tcttagagag gttaagatat acgtttgaag
     3001 gaaggcaaga agatatgttt gtaacaatag attttacata tgcagatgca cctataaaac
     3061 ttagaaaaga gcatgaagaa aactgtagag tcaatggaat agtaccaaaa gattcagatt
     3121 caatagaaat tattcctaga ttttataaag attgtatgga tttaaaaaaa taagttagac
     3181 ttaatattga tgttgatatt atgaattttt tatatttaag caattgaaaa ttttattata
     3241 gattcaattt tatatgtgtt taagtatgaa atttatagat agttgaaata attaaatatg
     3301 attgatgcat aggaatcatt actaagctat aattaattat aggggagttt aaataaatta
     3361 tgagttacac aattataaaa tgataaaaat agttatctgt tacaaaaaat gtatcttttt
     3421 aattaataaa gataatatga aaacttaaaa ggcaaataaa cttaaatata aaatactctt
     3481 aaataagata ggagaaaaat gtatgtctaa ctttattcta aatgggattt ttagaacagt
     3541 tatagtaagt agtctaagca ttcttctgat attgattttc aagaaaaatg tatttaaaag
     3601 atttagca
//
LOCUS       DAGSWI010000236         3544 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.241, whole genome shotgun sequence.
ACCESSION   DAGSWI010000236 DAGSWI010000000
VERSION     DAGSWI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.241"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            153..500
                     /locus_tag="MJF80_003783"
     CDS             153..500
                     /locus_tag="MJF80_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBZ0282384.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(650..1699)
                     /locus_tag="MJF80_003784"
     CDS             complement(650..1699)
                     /locus_tag="MJF80_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBZ0282385.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            2085..2819
                     /locus_tag="MJF80_003785"
     CDS             2085..2819
                     /locus_tag="MJF80_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBZ0282386.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDRYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(2962..3453)
                     /locus_tag="MJF80_003786"
     CDS             complement(2962..3453)
                     /locus_tag="MJF80_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBZ0282387.1"
                     /translation="MNNQQQEVYKKLDELNIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT         1259 a          475 c          488 g         1322 t
ORIGIN      
        1 taaggattgt atttttctaa ctataaaaat agtattatac ttatatttaa aaataaaaga
       61 attttattcc tattgtgtaa agtgagaata ctattttctt atattggtat aataatagta
      121 aatctataat atagaaaaga ggtatttcta gtatgcatga aaataaaaag aatctattag
      181 acggaagctt agctcaatat aatgatacag cagttccaaa aataccagta tttgctggta
      241 acgatataac ttcagaagtt tattatttta aacctaatca agttcttaat gctcatagac
      301 accctaatgg tgaacaaata tttgtgttcc taaaaggaga aggtaaaatg aaacttggtg
      361 agcatgaatg tgatgtaaaa aacggcgata ctgtatttgt accaacagga gagtggcatg
      421 aaattactaa tggaagcaat gaagaaatgg tagccgttca aattacaaaa attaatgctg
      481 gagcagaata cagaggatag atatataagt acagttagta ctttaatatc tattttatta
      541 tataaacttt aatctcaaat ataaaaagag tcttttaaaa ggctcttttt atatttttgt
      601 taattttcat tataaaaaca agaaaatatc tagctaaaac taatatattt tatttaatat
      661 tttttatagc attgtttgca aactcagtat ttacgatatc tttataaggt gcttccttat
      721 ctagttcttt agcttctttc ataacagtca tcaatttctt caaaccttcc tcttttaata
      781 caggattttg agaccatgca tctattgatt tatatctatc tacaactgtt gttaaatctt
      841 ctaaagatat atcttcaaag aaaggttgca tagccttagc tacttctttg ctagatgctt
      901 gttgaatcca taattgacct ttataaacag catttgtaaa tctttgaatt aaatctttat
      961 tgtttttcat gtatgatctc gtagtagagt atgctgtaaa aggtatttct ccagaatctt
     1021 ttcctataga tgtaactata tatccactac cttctttttc cattgctgtt gctgtaggtt
     1081 caaatgctgt tgtaaagtca ccctctccac ctgcaaaagc tccagccatt acaccaaatt
     1141 gtacatcagt tcttacatta acattgcctg cttttagatt ttctccaata gtaagacctt
     1201 tttcttttag aacatattca aaagtcatta gtggaactcc accttttcta ccacctaata
     1261 tttcggttcc ttttaaatca ttgtaagaga aattaggcat ttttttccta gcaactagaa
     1321 aactaccatc tctttttgtc aattgagcaa agttcactgc ataatcgttt ttgttttgat
     1381 tatatacata tatagatgct tcaggaccca ttaaacctac gtttgcttca cctgaaatca
     1441 aagctgccat agttttatct gcaccttggg tatttataag gtcaatcttt ataccttcat
     1501 cttcaaagaa tcctttcgta atagcagcat actgtggagc ataaaaaaca gagtgagtaa
     1561 cttctgctat agttacttta tccagatttt tagacgaaga agattcatct tttttgtcat
     1621 tagagttaaa atcacatcca gttagacata ttgtacttac aatgatagag cttgctattg
     1681 cgactatttt tttaaacata ataaatacct cccataagac atacgaatag tatatagtat
     1741 taaatattta ttgtagttgt tacaaaaatg tatctatact tataatattt ctacaaaaac
     1801 ttaataataa ttttcagttt tatgtttagt tttttcagta atgtgtaata ttgaataaat
     1861 tatatttatt atttataaac taagtaataa aattaaaata atatcacttt tttgtctatt
     1921 atatataagg aaaaattttt taataatata aatcatattc atttatatta ttaaaaaatt
     1981 tatttataga aacttttatt attttatata aaagtgataa aaacttatat tttgtattta
     2041 atagagtgtt gctaagtgaa tatatttaaa atggaggaaa ctttatgaaa caaaataaat
     2101 atgatgatga tagatttttt aataaataca gtaaaatgga acgttctaca aatggattag
     2161 caggtgctgg agagtggcat gttttaaaaa aaatgttacc tgatttcaaa ggaaaaagag
     2221 ttttagattt aggatgtgga tttggatggc attgtcaata tgcagttgag aatggagcaa
     2281 tttctgctgt tggagttgat atttcagaaa aaatgttaaa agaagctaga aacaaaacgc
     2341 aacttgataa tataaattac atatgtatgc ctattgaaga tattaatttt ccaaaagata
     2401 gttttgatgt agttattagt tccttggcat ttcattatat tcaatcattt gaagatatat
     2461 gtaagaatat aagtaattgt ctttcaaata aaggtaattt tgtattttct gttgaacatc
     2521 ctatatttac tgcatatgga agtcaagagt ggtactgtga taaagatggc actaatctac
     2581 actggccagt agatagatat tttgaagaag gagtacgtaa gtcaaatttt ctaggagaag
     2641 aggttataaa atatcataaa acattaacaa cttatttaaa tacgttaatg aaaacaggat
     2701 ttgaaatatt agaaattatt gaacctcaac cagaagaaaa actgttagat acaattcctg
     2761 atatgaaaga tgagctacgt agacctatga tgttacttgt atctgcaaaa aaaagataag
     2821 taaatataaa taatcattat tgtagaaaaa aagttgtaga aaataattac actaatggag
     2881 atgattatac cagtggtatt aattacatca atagtaagga ggcaaaagca ttcgttgctt
     2941 ttgccatttt ttaagttgca attatacttc tacgtagtat atttcgtttc cattttcttc
     3001 tataagcttt ttcatatctt caaattttaa aaatacagta gcagtattat catttggatg
     3061 tatacctatt ttatccattt ttaataaatc cttatcaaat acaactttta caacagcctc
     3121 tttatcattt aatataccaa aaggagtcac agctccttta gtaagtttca agtatttttg
     3181 aagtctttct tctgatgcaa aactaagagg tttactatca atttgttgac gaatatcttt
     3241 taaatctgct tttttgtctt tacctaatac aactaaaaaa tgttcttttc ctttagcatc
     3301 acgaagaaat aggtttttta caacaaattc catatgtaat gtttttaatt cttccatttc
     3361 ttcaattgta taaactggtg gatgatttac agcctcataa ggtatgttta actcatctaa
     3421 ttttttatat acttcctgtt gttgattgtt catgatttcc tcccattaga aatttatttt
     3481 atatataaat aatattatca cagaaaactt taaaagaaaa cgttttatat aatatagaca
     3541 atag
//
LOCUS       DAGSWI010000237         3541 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.242, whole genome shotgun sequence.
ACCESSION   DAGSWI010000237 DAGSWI010000000
VERSION     DAGSWI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.242"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            351..1163
                     /gene="modA"
                     /locus_tag="MJF80_003787"
     CDS             351..1163
                     /gene="modA"
                     /locus_tag="MJF80_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBZ0282388.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            1173..1964
                     /locus_tag="MJF80_003788"
     CDS             1173..1964
                     /locus_tag="MJF80_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282389.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            1966..2661
                     /locus_tag="MJF80_003789"
     CDS             1966..2661
                     /locus_tag="MJF80_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0282390.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAKPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2648..3379
                     /locus_tag="MJF80_003790"
     CDS             2648..3379
                     /locus_tag="MJF80_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282391.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
BASE COUNT         1398 a          444 c          510 g         1189 t
ORIGIN      
        1 tataacactt ttagtaattt attaataaaa aacaattaaa acatatctat atgtttcaat
       61 gttctatttt ttcactgaaa tataataaat ataaaattag ttaaaaagta aaaaatctac
      121 aataagttaa tttgatttaa gtttttaaat ttaaaatact ttaaagtatt aataaaaatt
      181 aaaaataaaa taactaccta tggaaatgac attataaata gattttttct taactataca
      241 taacaaaaca taacaaaaca ttataaaaca taacaaaaac ttagacatcg ttttcttagt
      301 atgatatact aaaaaagaat ttacactaat ttactaaggg ggaatatctt atgtttaaaa
      361 aactttcagt actagcttta gctatgatta tttcattaag tattactgca tgtaattcta
      421 aagaaaacaa agctaatgat gaatctaata ctaaagtgac aacagctgat gattcaaaag
      481 aaaaagagac tctttttgtc tattcagggg caggtttaaa gaaaccaatg gatgaaatag
      541 caaaaaaatt tgagaaagaa aataatgtca aagttgaata tagctatgca ggttctgcac
      601 agctaatagc tcaaattgag acaagtcaca aaggagacgt atttattgtt ggttctgaac
      661 caatttattc aaaggccttt gaaaaagaac ttgtaggtga acataaaacg gttgcacatc
      721 atacacctgc aatagttgta cctaaaggaa atccagctaa tattcaaaaa ttggaagact
      781 taaataaaga tggagttaaa cttattttgg gagacaaaga gtctaatgct ataggtaaaa
      841 ctacacaaaa aatacttgaa aaaaataatt taaaatctat aaatgataat gttgtctcta
      901 cagctgcaac tgtaaatgaa atgattgtac agttaacatc aagtaaagct gatgctacaa
      961 tagccacaaa agattcggtc tttggtaatg aagatgtaga agttatagaa attgcccaag
     1021 ataaaaatat agaccaactt atatcagcag gaatcgtaaa ctatagtgat aaaaaagatt
     1081 tagctaataa atttataaat tatatagagt cagacgaagc taaacagata tttaaaaaat
     1141 atggttttga acctgttaag tagtgattaa acttgaaaaa taaatttttt actactctac
     1201 ttactggatt ttttgctata gtctttttgt ttattgtatc accacttctt atgttaatca
     1261 ttaaagggat aagttatgtt cctatatgct taaaatcagt ggaagtacag tatgcaataa
     1321 cacttagtat taaaacatct ttaatatcta ctatagtatg ccttttatta gctattccag
     1381 tagcatattt tttacatatt actaaattgc catttaaaaa actgattata cagataataa
     1441 atctccctat gtctcttcca cacttagtat caggaattgc tcttttactt ttgtttggaa
     1501 gaatggggat tggagattcc atttataaga tttttaagct tgattttata tttacaaagc
     1561 aaggtatagt tttagcacaa gtatttgtaa atcttccact tactattaaa atacttcaca
     1621 cttcactaaa tgaaagtaat gaaaagatga tatttgttgc acgtaccctt ggatgtaata
     1681 gttgggaagc atttagattt ataattcttc caaatctaaa aactggaatc atatctgcaa
     1741 ctgtaatgac ttggtcaaga gcacttggtg aatttggtgc agtagctatg atagcaggtt
     1801 ctacacgtat gaaaactgaa attattccta catctatata tttaaatatg tctactggtg
     1861 atatagacat tgcaatcgga atagctgtaa ttttgatatt tatctcactt acatgcttga
     1921 tgctttttga gatatttttt aatagagagg ttgataagaa ttaatatgtt aaaaataaaa
     1981 aatttatctc ttgatttacc taattttaaa ttacaagaaa atagtttaaa tattaataat
     2041 catgaatact ttgttttatt aggagctagt gggtcaggta aaactctatt cctagaaaca
     2101 ttagcaggaa gatatccatc tgctaaagga aatgtatcat acaaagaaaa tttaatatca
     2161 aacttacctc cagaaaatag aaatatagga tttgtatatc aaaattttga attatttcca
     2221 aatatgaatg ttgaagataa tataaggttt ccacttaaat taagaaaaat acctatagat
     2281 gaacagattc ttaaggctaa ccacttgtct aaattaatgc agatagaaga tataaaaaat
     2341 aggtatccta aaaatttaag tggaggcgaa aagcaacgag ttgcttttgc acgtgcctta
     2401 atagctaaac ctgatatatt acttttagat gaacctatga gttcattgga ttatattaca
     2461 aaaaaacata taagcaaaat tttgaaggat gtctacataa aatacaaacc aacagtgatt
     2521 catgttactc atgatatttc tgaagcaatg ttttttgctc ataagatagg aataatgaac
     2581 aatggaaaaa taaatcatat ttttgattta aatgaagaaa taaaaaataa aggagaatct
     2641 tttttctatg aatatatttg atattttgag agattatcta ttagtacaag tcgataaata
     2701 taatttaaat atggatatga taagtattgt gtccaaatct ctatcttcaa aagaagcaat
     2761 tggaaatact aagcgtaaag attttccaat tatagttggt aaagaaataa tgcttgaagc
     2821 tgactttaaa ggagctaaag gacaagcatt tacttctact ccatctacat ttgaaggttc
     2881 tttaaaagac atattatcat tagatttaca tgacaatcct catgatagaa gtctttttat
     2941 agcatcatta aatgcagtta tgaaatactt aggcaagacg gatagaacca ttcattgtaa
     3001 aaataacgag ccagaggtat gtgcaaaaaa attccctgag tttataaaaa tggaatttgg
     3061 taatccaaaa gtagcaatta taggatatca accagccata attgataata ttaaggactt
     3121 tttcgaaaca agagtattag atttaaatcc tgaatttgtg gatactattc aatataatgt
     3181 taagattgaa gatggtatac gagattatga agatgtaata tcttgggctg atttagtgat
     3241 ttgcacagga agtacattat gtaataactc aattataaat tttctatcac ttaacaaacc
     3301 agtttactat tatggaacta cgatagctgg tgcatcaaat attttaggtt taaaaagact
     3361 ctgtttttgt agtaaataag caatcatatc atcaaggagt acacaaatat atatgtgtac
     3421 tctttaaatt taaaaagtta ttacttttat tctaagtact aaagtaaaat tctattaaaa
     3481 tgaatcccga ataaatgtac aatcccttta aatctcaatt cctatcaatc attaatattt
     3541 t
//
LOCUS       DAGSWI010000238         3536 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.243, whole genome shotgun sequence.
ACCESSION   DAGSWI010000238 DAGSWI010000000
VERSION     DAGSWI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.243"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            228..605
                     /locus_tag="MJF80_003791"
     CDS             228..605
                     /locus_tag="MJF80_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0282392.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            598..1746
                     /locus_tag="MJF80_003792"
     CDS             598..1746
                     /locus_tag="MJF80_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HBZ0282393.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLTDCSLKDKKEYALALIKSIKLSKNYNSGILTTEFNKTNLE
                     KRLESIVKKKGRSGILIAILVIMTSITSFIEVDAQIKINNSINEINNNINDFILNKST
                     DKHQTFYYKDAPSKIRKFYEDNCKLKGTTPKDYDTIIINTKDFNELMK"
     gene            1763..2362
                     /locus_tag="MJF80_003793"
     CDS             1763..2362
                     /locus_tag="MJF80_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBZ0282394.1"
                     /translation="MLKKSKKTLAIMCCLMLTFTSVAFADASKGEGVSKEAVSEKNTY
                     SFNISGYVYTYENAPQKIRDEHKQNCEDVNIAPKPDDKIFVPTSANLMNDDDKAVYTN
                     YAVAYKPDEKTITVIGGGEFITISTTKVIRKGDTGKEVTVAQIMLSKLGYNTSPDGIF
                     GSKTYNAVVSVQKKYGLSADGVIGPATWDKLGRLTDSSF"
     gene            2500..3536
                     /locus_tag="MJF80_003794"
                     /pseudo
     CDS             2500..3536
                     /locus_tag="MJF80_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         1459 a          342 c          563 g         1172 t
ORIGIN      
        1 tttattaaat agttttaatg tgtaaataac attctacact attttaatta aatatgtaaa
       61 aagtaaaata tttaaataga tatattttaa aatctaactt ttaatattgt aaaaaaatta
      121 gaaatttaac aaaaagtaca aaaattcaca tttttttcat aaagtgtttg actaaaaaat
      181 tcacatgttg tagaattaag gtagcaggag ttttgaggga ggtgtaaata atgataagaa
      241 aaataccaca agcagaacta aaagtaatga aattcatttg gaaagtagat gttacagtga
      301 catcaaaaga tgttattgaa gctatggaac aaaaatatgg ctggaaacaa accaccacac
      361 tgacactttt atctaggctg gtaaaaagag gattcttaga tgctcaaaaa atagacagat
      421 atacacatta cacaataaga gtaaaacaca aagattattt aagttttgag acaaaggatt
      481 tcctaagtaa tatacatgat gactctttga gaagtttaat ttcagcatta catgatgatg
      541 aaaatatagg taaagataag ctagattttc tagaaagtta ttttgataat ttaaaggatg
      601 aataattata tgctatatgt ttttgaaaat cttataaaga caactattgt atgtagtctt
      661 ggtatttgtc tattattatt tttgaaaaga tatttattta agaagtttag taagagattc
      721 aattattata tttggttgat aatagtcttt agaatgttac tttttctatt taattataca
      781 atagtatatg aagtaaaaga acccaaagaa aatgcattag gaaataatat tacacaaatt
      841 gatatttcta cagataataa tcttatgctg tatgtagctt atttatggtt atttgtaact
      901 atagtcattg cagtttatac atttataaag tatacaagat ttaaaaatct agttgttgat
      961 gtatcttatg atattgaaga taatgatata aactgtatct acaaaaactt gttaaaagaa
     1021 ttaaatataa aaaagaaaat tgaattaaga ggttctgatg aattgataag tccagcaggc
     1081 atgggattat ttaaatctta catattctta ccagactatc cttatagtaa agatgaacta
     1141 acttggattt tgaaacatga attaatgcat tttaaaaata aagatatttt aataaagttt
     1201 ttagttttgt ctgttagaat aatatattgg tttaatccac ttgtatatgt aatgagcaat
     1261 aaagtaaatc tagattgtga attatgttgt gatgaaagtg ttttgactga ttgttcttta
     1321 aaagataaga aggagtatgc attagcactt ataaagtcaa ttaaacttag taagaattat
     1381 aatagtggta ttttaacaac tgaatttaat aaaacaaatt tagaaaagag acttgagagt
     1441 attgttaaga agaaaggtag aagtggaata ttaattgcta tattagtaat tatgacttct
     1501 ataacatctt ttattgaagt tgatgctcag attaaaatta ataatagtat caatgaaata
     1561 aataacaata ttaatgactt tatactgaat aagtcaactg ataaacatca aactttttat
     1621 tataaagatg caccaagtaa aattagaaaa ttttatgaag ataactgtaa attgaaaggt
     1681 acaacaccta aagattatga tacaataata attaatacta aagatttcaa tgaattaatg
     1741 aaataattat aaggaggatt ttatgttaaa aaaatcaaaa aagacattag ctattatgtg
     1801 ttgcttaatg cttactttta caagtgtagc atttgctgac gcaagtaaag gagaaggagt
     1861 gagtaaagaa gctgtttcag agaaaaacac atattctttt aatatttctg ggtatgtata
     1921 tacatatgaa aatgctccac aaaaaataag agatgaacat aaacagaatt gtgaagatgt
     1981 aaatatcgca cctaagccag atgataaaat ctttgttcca acatcagcta atctaatgaa
     2041 tgatgatgat aaagctgttt atacaaatta tgctgttgct tataaacctg atgagaaaac
     2101 cataacagtt ataggtggtg gtgaatttat aactataagt acaacaaaag ttatacgtaa
     2161 gggtgatact ggaaaagaag ttacagttgc gcaaattatg ctatctaaac taggttacaa
     2221 tacaagtcca gatggtattt ttggaagtaa gacatataat gcggtcgtaa gtgtacaaaa
     2281 aaaatatggt ttatctgcag atggagtgat tggtccagct acttgggata aattgggtag
     2341 attaactgat tctagttttt aataggatta aaatctaata ttaaatagtg ttccaatgtg
     2401 atttcttaag agcttttaaa ataaagtgtg tattcttcta aaaagtgatg ataattaaaa
     2461 tataaaatat cactatacaa aggaaggtac acacttttta tgtttaacaa aaacgaaata
     2521 ttaaaaaact tattgaagaa tatgatgtca aaacaactac aaatattcaa gacatactaa
     2581 aggttttatt tgcaagtaca attcgtcaaa tgttggaaac ggagtttgat gactatttag
     2641 gttatgataa taagaataaa actgctaaaa actctagaaa tagatataga aataaaaatg
     2701 ttgaattaga ttttggagaa ataactttaa atattccaag agactgaaat tgagattttg
     2761 aaccaaatgt aattagaaac tatgaaagtg atatatctga aatagagaac caagtgattg
     2821 gaatatactc aaaaaaggta tgtctactca agatatatca aggcacatta aaagtattta
     2881 tggaatggac atatataatt aattaatagc taaagctact gatatagtta tatctttagc
     2941 tcatgaatag caaagtagac ttcctgatat gatttattca attgtattta tgatactata
     3001 tagtataaag ttataagtta aaatagaata aataaaaaag cagcatctat agctacaggt
     3061 attaacttaa aagggataaa tgaggtttta tgaaccatgg gttggagaaa tgaaagttta
     3121 aaatgttgcc taaaggtact tacagatatt agaaacagag gagttaaaga tatattaata
     3181 gcttctgtag atggattagt tagattttct gatgcaatta aagcagtatt tcctgatact
     3241 gaaatacaaa gatgtgttgt gcatcaaatc agaaatactt taaattatgt atcatataaa
     3301 aatagaaaaa aatttgcaag aaatttaaag aaaatatagg attcaattat agaagaataa
     3361 gcgttaagta agctattgct cttaaaagaa aaaaggggca atcaatatgc gatagcatta
     3421 agaagttggt gaatcagttg aaatttacaa tagattatta agaaaagtta ctaaatcgaa
     3481 gtctattttt ccttcagatg attctttgca caaatcatta tacttagcaa ctatat
//
LOCUS       DAGSWI010000239         3510 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.244, whole genome shotgun sequence.
ACCESSION   DAGSWI010000239 DAGSWI010000000
VERSION     DAGSWI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.244"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..1152
                     /locus_tag="MJF80_003795"
     CDS             <1..1152
                     /locus_tag="MJF80_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282395.1"
                     /translation="AIYFSIGLLNNEDNLKIKTIKGDSNELDHININFGYALNGYINS
                     YYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVGFEMNEESKDINFVIDE
                     KKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDDYYANILASKRYKKYLY
                     VSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLSNYFSEKYQNIETIGVS
                     YASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESIMELKTDSKMALAKAKL
                     EANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDNREKRGIRSIEIDSNKI
                     VAYTSRLNSILSWDNKFMLYIINKNSKEIVYEGELQFNSSNSMLEFEIK"
     gene            1161..1973
                     /locus_tag="MJF80_003796"
     CDS             1161..1973
                     /locus_tag="MJF80_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282396.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVKVTGFSSFRNIMGSTESWFMFGIIACICYSFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            1984..3252
                     /locus_tag="MJF80_003797"
     CDS             1984..3252
                     /locus_tag="MJF80_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282397.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT         1506 a          271 c          520 g         1213 t
ORIGIN      
        1 gcaatatatt ttagtatagg tttattaaat aatgaagata atttaaagat aaaaactata
       61 aaaggtgact ctaatgaatt agaccatata aacataaatt ttggatatgc tttgaatgga
      121 tatataaatt catattatac aataaatgga agtaacaaaa ccaaaaagac tgaattaata
      181 aaactacaaa aggatagtaa gaacacaaat aatttaattg atgtatctag tggttcagat
      241 ttaattgcac atgttggatt tgaaatgaat gaggagtcaa aagatataaa ctttgtaata
      301 gatgagaaaa aagaagattt agaaaaaggt gtatttccat cagaaggaag tgataaagaa
      361 attgaatatg aaaaattaga tatgaataat tttacaagga caagatataa agtagataaa
      421 agtacagatg attattatgc aaatatacta gcatcaaaaa gatataaaaa atacttatat
      481 gtatctgtac tttatgctac aagtgattca tcagaaaata acaaagaata ttcaagtact
      541 atagatataa taaaaattaa tccagaaaat aaaaaaatca agactataaa aagtataaat
      601 ttaagtaatt atttttccga aaaataccaa aacatagaga ccataggtgt aagttatgct
      661 agcaaatatg aggataagat gtatatttta gtaaatgtac taaatacaaa cataggtgat
      721 aatagtaatt ctaaatttca tttacttgag tataatcttg aagaaaataa ggaaagcata
      781 atggaattaa aaactgatag caaaatggct ttagcaaaag caaagttaga agctaataaa
      841 ttatatatgg taatgtcgaa tgattatgat aatagttcaa ttacagagtt aaatttactt
      901 acctataata tattagataa aagcttagat gaagataaat atgttttaga taatagagaa
      961 aaaagaggaa ttagaagtat tgaaattgat tcaaataaaa tcgttgcata tacatcaagg
     1021 ctaaactcta tattatcttg ggataataaa tttatgctat atataataaa taaaaattct
     1081 aaggaaattg tatatgaagg agaattacaa tttaacagtt caaatagtat gttagaattt
     1141 gaaataaagt aggtgagttg atgaaagatt taataagatt attcgaattt gaactaaaaa
     1201 gaaatataaa gaattatata tttataataa tttgttgttg ttcttttgta attttaaaca
     1261 tagtaaaaaa tttaaatgac tataattaca taatagaaaa tgcagttaaa agtgaacagt
     1321 taacaaaaat cggaaatacg atagaaacgg taaaggttac aggtttctca agctttagaa
     1381 atataatggg ttctacagaa tcgtggttta tgtttggtat aattgcatgt atttgctatt
     1441 ctttttttat ttggtataga gactttaatg gtaggagtaa atctatatat acacttataa
     1501 tgctacctaa aaatagaatc aatatttata tatctaaatt attaaatata ttatttctag
     1561 tgtatagcta tacagtagta cttacaatat ctttgtttat tgcaagtaag ttgttgccta
     1621 gacatatgct tggtaatgtc acaaactatg gttttgtaca agaaacaata tatgagttaa
     1681 aaatgttact tccatatagc tttgaaatac tatttgtaga gtatatattt ttattgatag
     1741 gatttgtatc tgtagtattt acatcaattc ttataaataa atctatttat aaagtaagca
     1801 ctttaataag ttttctattt ttagttatag aaattttagt gtttataatt tctatagaat
     1861 ttattatacc ctacgaatac agtgatgtat ttactgttct atattcaagt ataaacatat
     1921 ttgtatgtag tcttatatct aataaacttt tgaagcaaaa gattgacttt taggagggat
     1981 attatgaaag caaaaaatat aggaaaatat acaatatgtt ttataatagt atgtttttta
     2041 atgattacta tgaccttaat attgagttat tttaataagg ataaactatc tgtaaaaact
     2101 ttaaaaggca atgtaaaaga tatagggaga gtaagtgtag tatatacacc taatataagc
     2161 aaattttctt atgaggaggt aattataagt aaaaatggag taagaaataa tacaaaccca
     2221 aaatcaagaa ttagaaatga ttatttaaat attgacaaaa aatacagtga ctttgttaat
     2281 gataaaggaa aagaatttat atataaagat gatttgaata taggagaagc gtatacctct
     2341 gttaagtctg atttagtaca aggaatgaat gctttaaatg attgtataaa ggtttcaaat
     2401 aaaaatttaa aaagtggggt agaagaaaat tttgatatta atttaaataa gtctgatttt
     2461 aaagaaaata gttggaaaac cttatttaac aaaatatata aaggtaattt atatatggta
     2521 attagtgaaa ctaatatttc aggttctaaa gtcaataata tttgtatact aaagatagat
     2581 ataaaaaata agtcttataa attattaaat agatttaatt taggattaca gaatggagaa
     2641 caacaaaata tcaatgaaat taaattttct attggaagta aattatactt agaaatagta
     2701 aataataaaa gtaaaaatgg atatattgaa ggacatgata gttactctaa ctttattata
     2761 tatgatatag aagataacac ttttaatata agtaataaat ttatggataa tctaaacaaa
     2821 gaaaagaatg caaatccaaa ggagattggc ttattaagta aaactaagct agattatatg
     2881 gtaaaaaata ataaattgaa tcttatagtt acggatatga gtaattccat aactaatctt
     2941 gtttatgata ttaattctaa tgagattaaa ttagaagatt atgagatact tgatttaaaa
     3001 gtagattata atgaagcatc atctggagtt aggaaagtta agctactgga tgataagata
     3061 tattctattt caggtattaa gaacagtata tcaggagaat taaaaggatt taaagggatt
     3121 gttgatgtag tcggaactaa gccagtaaaa tttcaagtat ttgatatgaa acttaaaaag
     3181 aatatttatg aagctgagct aataaatgga gatataagtt tacaagaaaa gttatttttt
     3241 gttgcaaatt aagtgttaga attaatttta gaactttaaa cttttatatg tatcacagat
     3301 aagtataagt aagggagtgt ctcaaaataa actttttagc tcatgagaca ctttttttat
     3361 atgaaatcaa atatattttc attgtttaga tttattttga taatgaaaaa aagattatct
     3421 ttattttaag aaagacattt atttataatt atatacaaaa atatttttta atgtttttgt
     3481 gtataattat aacaatttaa tttatattaa
//
LOCUS       DAGSWI010000240         3504 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.245, whole genome shotgun sequence.
ACCESSION   DAGSWI010000240 DAGSWI010000000
VERSION     DAGSWI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.245"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(5..706)
                     /locus_tag="MJF80_003798"
     CDS             complement(5..706)
                     /locus_tag="MJF80_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBZ0282398.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(984..2369)
                     /locus_tag="MJF80_003799"
     CDS             complement(984..2369)
                     /locus_tag="MJF80_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBZ0282399.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(2477..>3504)
                     /locus_tag="MJF80_003800"
     CDS             complement(2477..>3504)
                     /locus_tag="MJF80_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBZ0282400.1"
                     /translation="IDNYIPYDQGITLDIFMYIWILGIIAISFYYLRTNFKFYNYIEN
                     FKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTRCKIIIPEKKYNNK
                     ELRFIFKHELIHYKRKDNLFKLLISTICIIYWFNPILYLLKKYFHELCELSCDEIVID
                     KCTTSEIKEYAYIILDTIKYQKTLKYSSFVSQFLNKKNIILKKRLDSMFNQDKKKINL
                     GLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGTHIIDSNGNSIEPE
                     LIQTIGRGKKVGYVKKRNLYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKI
                     IGRFEIN"
BASE COUNT         1206 a          494 c          428 g         1376 t
ORIGIN      
        1 attattattc ataagattcc tcctttttac agtaagattc ttctataaag aaaccaatta
       61 aagatgctaa aattgatgca actattatta ctaaattgct tttgaataat gcatataaaa
      121 taacagaaat aatgattgat gatataaata ctacaatata aatcttattt tttatttgag
      181 aaactattaa agctataaac atagaagtca atatgaagcc agaaatagaa ctgtctatag
      241 atatactttc tcctaaaaat gctccaataa aattagctaa aactgatgaa attaagcaga
      301 agaaattaag ataaatagca tttctatcag accaatttcc agcagtaaat ttttcataat
      361 taactgcaaa agtttcatct gttatagtat gacaaaatgg caatataaat tttggacttt
      421 tattttttat gtacatgttc aaattagaac tcattagaaa atgtcttaag ttaactataa
      481 atgtagtcaa aacaatagat gttacacttg caccagtaga aatcattgaa gctgcaatga
      541 attgagaact tcctccaaat actaaagtta ccattagtgc tatttgaaaa ggtgaaagac
      601 cagatttttg taatagtatt cctcctacta tacctatagg tatataacct aagaatatag
      661 gaattgaagc aataaagctt tcttttaatt gttgagttct ttccacaata aatcactcct
      721 tttgtattaa atgtattaaa gtattagtaa aacagaaaaa tagattaatt gtataattgc
      781 aattataatt aaaaataaat ttggattaat gtataaaata ctatacaaca aataaataat
      841 cttaaacggt tattaaattt tatctatttg ttattatgat gtaataatac atattaatct
      901 ctataagtta attactatct ggtgtaattt tattttaaca acaaaaaaaa atacacacaa
      961 ttaaaatatt gtgtgcaata caattatttt ttctcttcta atagtctttt taatacgctc
     1021 aaaccagctt taagttcttc tgtactttct gtagcacata cagctattct tattgcagaa
     1081 attggttttt cttttccaac agcaaaacgt tcagaagcat agacctgtac accttcttta
     1141 agtgcttgta tctcaaattt ttctgccatt attccatcag gtagaatcaa ccatctaaat
     1201 atacattctt catttcccaa aagattatat ccagataaat attgattaat aatcttgttt
     1261 cttcttcttg cagatttacg gcgtgcatta attaatttat cggcttcacc tgatgtaatc
     1321 atgcgataag caatttctgt taaaaaataa gattgagata gattaatgtt gtataatgcg
     1381 cttgataatg cctctcgata ctgtttaggt gtagagatat atgctagccg taaactaggt
     1441 gcaataattt ttgataaact cgtaatataa attgtttgat tgggcagata ggaagccact
     1501 ggatttaaat gtgtttcatt aagtaaacta tgaatagcat cctcaataac aattaaattg
     1561 tatttagatg caatatttgc aatcattttt cgtccatttt gtgacattat atgtgtagtt
     1621 ggattttgat aatctggtat tatgtataga cctttaatat tttcattttt acaagcatat
     1681 aatagtcctt cttcactaat ctcattatct tcttgtttaa ttggaattag ttgaatccca
     1741 agcattttag ctgctgtctt tattccagga taagttaatg ggtctacacc tattctatct
     1801 ccatgttgaa acaaaccagc taaaatggca acaattgcat tttgtccacc acttgcagga
     1861 agtaagctat ctgatgtagt ttcaagccca gctttataaa ttaatttggc aatagcttct
     1921 ttttgccatt tagcaccatc tctttgtcca tattggaata tggttttaaa atctatttcc
     1981 ttaattatgt gtttaaacaa agtattcatt tcttctagcg tgaaatctgg attcattgtg
     2041 cccatttcaa taaatgtaat tttattatta ctggacatca agaatagatt tgaacgtgta
     2101 tcataagata caaaagttcc acttcctgta actccactta ttagaccttt tttttcacaa
     2161 attttaaaag ctctagagat agtactcaca ttaacatcta agaaatctgc caattcacgt
     2221 tggggtggta atttagtacc tggtaataaa aatccattgt tgatatcttg ttctagttgt
     2281 tctgcaagag ccttatataa tatttgacct tttgaaggtc tctttggttt ccagttcata
     2341 ggataatttt caaaagaatt aattggcata agttttcacc tcacattatt aatttgattt
     2401 tttctttatc taaaacaata aattctaaag atacaaataa aaactgatag gaaataattc
     2461 tatcagtttt tgtcttttaa ttgatttcaa atcttcctat aatttttata ccttctttat
     2521 cataaagagg gattgttttt ttaaatatat taaaatttct ttttcttgta tataaaacag
     2581 cttcttctgg tgtctttggt tgattatcac aatcgtacaa atttcttttt tttacgtagc
     2641 cgactttttt acctctacct attgtttgta taagttctgg ttcaatggaa tttccatttg
     2701 agtctattat atgtgtacca taggtttctc catttttatt ataattaatc ttaccaatag
     2761 tatattctac atcttcattt tgtttaaaat tggagttgat agaaaatatt gaccctatta
     2821 ttataaaggt taataaaaac ttaagaccta aattaatttt ctttttatct tgattaaaca
     2881 tagaatctaa cctctttttt aaaataatat tttttttatt tagaaactga gatacgaatg
     2941 atgaatactt caaagttttt tgatacttta ttgtatctaa tattatatat gcatactctt
     3001 taatttcact cgtggtacac ttgtcaatca caatttcatc acaagataat tcacataatt
     3061 catgaaaata tttttttaat aagtataaaa ttggattgaa ccaatatatg atacaaattg
     3121 tactaattag aagcttaaat aagttatctt ttcttttata atgaattaat tcatgtttaa
     3181 agatgaatct taactcttta ttattgtact ttttttctgg aattattatt ttacagcgtg
     3241 ttaaacctat aatggcagga gaataaatac ctttaacctt atatatttga agttttttat
     3301 ttatacttag ttcttttgct tgttcatcta agatactagt aatatctatt tctaaaattt
     3361 cttttttaaa attttctatg taattataaa atttaaaatt tgttcttaaa tagtaaaaag
     3421 agattgctat aattcctaat atccagatgt acataaatat atctaaagta atgccctggt
     3481 catatggtat ataattatcg atat
//
LOCUS       DAGSWI010000241         3312 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.246, whole genome shotgun sequence.
ACCESSION   DAGSWI010000241 DAGSWI010000000
VERSION     DAGSWI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.246"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            228..1547
                     /locus_tag="MJF80_003801"
     CDS             228..1547
                     /locus_tag="MJF80_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBZ0282401.1"
                     /translation="MKRVYQHINVYEALQERLKFIFQEFENIFVSFSGGKDSALLLHL
                     VIDYKRRNNLDTKIGVFHQDFEAQYQITTEFVEEIFEKYILDIEPYWVCLPMAVRTAL
                     SNYQLYWYPWDDKNKEIWVRDLPEKPYVIHMGNNPFYLYKYRMLQENLAKQFGRWYKQ
                     VHGNKKTVCLLGIRADESLQRYSGFLNNKNGYKGECWLSKKFKDVWTASPLYDWKITD
                     VWTAYYKFGYTYNKLYDLYYKAGLKPSQMRVASPFNDYAKESLNLYRILEPKTWTKLV
                     GRVQGVNFTAIYGKTKAIGYRNITLPEGYTWQEYTKFLLATLPARVRNSYIKKFNTSI
                     KFWHKTGGGLSEETINELLENGYKIKRNGVSNYTLDKKSRIVFMQKIPDDTDNIKSTK
                     DIPSWKRMCYCILKNDHLCRFMGFGLKSDEQRKIDTIRKKYKGLEEI"
     gene            1547..2080
                     /locus_tag="MJF80_003802"
     CDS             1547..2080
                     /locus_tag="MJF80_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBZ0282402.1"
                     /translation="MDFKSPVYNIVAIPVEKIVPNTYNPNAVAPPEMKLLYDSIKEDG
                     YTQPIVCYYSQKKDKYIIVDGFHRYRIMIDYLDIYEREKGKIPVVIIDKPLDCRMAST
                     IRHNRARGSHDVDLMSNIIRELHEIGRSDAWISKHLGMDKDEILRLKQITGLTYLFRD
                     VEFGKAWIPEETIYNEE"
     gene            complement(2219..>3312)
                     /locus_tag="MJF80_003803"
     CDS             complement(2219..>3312)
                     /locus_tag="MJF80_003803"
                     /inference="COORDINATES: protein motif:HMM:NF016901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0282403.1"
                     /translation="SLSTLNRKINECNKLLNSFELRIKKFDLKGSPLQFAYFYYSLFR
                     NSGVNKEITPGLNDKDIVKFLEDSMSIDFTYAQKLSIYIWSKIIIKNRDKFTKENFSD
                     KFSLSNLDSFKNDITFKNLELFYSKFPHYKKYLAYATACFLKTFGVLAFENTVDITLP
                     FNVHNFILSRMKSLFLNKDFEFDKTVKSNILSYCNKQFYFKGIFYSIDENTRDFYLNS
                     HLSFLKDYFIVDLFKDIKLSFKLDNLDFDYFKLLIVLNLSYIDEESKYTIKIGVLSKT
                     ENLAFKVLLKDFNNLLSKKFNAKAEVLSEKNLHTYDLIISNIHDQFSDGMSTTIFKFT
                     YLGVEYDLNNLTEILNNIEKEKIENLRLG"
BASE COUNT         1296 a          358 c          522 g         1136 t
ORIGIN      
        1 taaaaattca aaaaaattat tctattaata aaaagttgaa aaaattaaat gttttaaagt
       61 gatatcatat aatagaaaaa attaatttag aagtctaagg gatttaatat tatatgaaaa
      121 acagatatac ttcatctaat tattataatt ttaacaatta gataaagtta atgtaagtta
      181 aattaccaga tatacttttt aatactatat aaataggagt gaacaatgtg aaacgtgtat
      241 accagcatat aaatgtttat gaagcgcttc aagaaagatt aaaatttatt tttcaagagt
      301 ttgaaaatat ttttgtttct tttagtggag gtaaggatag tgctctttta cttcaccttg
      361 taatagatta taaaagaaga aataacttag atactaagat aggtgtgttt catcaagatt
      421 ttgaagctca gtatcaaatt acaacagaat ttgtagagga gatttttgaa aaatatattt
      481 tagatattga gccttattgg gtatgtttgc ctatggctgt aagaactgca cttagcaatt
      541 atcagttgta ctggtatccg tgggatgata aaaataaaga gatttgggtt agggatcttc
      601 cagagaaacc gtatgttatt catatgggaa acaatccatt ttatttatat aaatatcgta
      661 tgttgcagga aaatttggct aagcaatttg gaaggtggta taagcaagtt catggaaata
      721 aaaaaacagt atgtctttta ggaattcgtg cagatgaatc acttcaaaga tatagcggat
      781 ttttaaataa taaaaatgga tacaaaggag aatgttggct ttctaaaaag tttaaagatg
      841 tttggacagc ttctccctta tatgattgga aaataactga tgtttggaca gcttactata
      901 agtttggata tacatataat aagctatatg acttatacta taaggctggc ttaaaaccaa
      961 gtcagatgag agtagcttct ccatttaatg attatgcaaa agaatcacta aacctttata
     1021 ggatattaga accaaaaact tggactaaac ttgtagggag agtacaagga gttaatttta
     1081 cagctattta tgggaagact aaggcaattg gatatagaaa tattactctt ccagaaggat
     1141 atacgtggca ggaatacaca aagtttcttt tagctacact tccagcaagg gttagaaata
     1201 gctatataaa aaagtttaat acttctatta aattttggca taaaacaggt ggaggattat
     1261 cggaagagac tattaatgaa cttttagaaa atggatataa aatcaaaaga aatggagtat
     1321 caaattatac attagataag aagtctagaa tagtttttat gcaaaagatt ccagatgata
     1381 cagataatat aaagagtaca aaggatatac cttcttggaa gagaatgtgt tattgtattc
     1441 taaaaaatga tcacttatgc cgttttatgg ggtttggact taaaagtgat gaacaaagga
     1501 aaatagatac aatacgaaaa aagtataaag gattggagga gatataatgg attttaaaag
     1561 tccagtttat aatatagttg caataccagt tgaaaaaata gttccaaata catataatcc
     1621 taatgctgta gctccgccag aaatgaagtt gctatatgat agtattaaag aggatggata
     1681 cactcagcct atagtatgtt attattcaca aaagaaggac aaatacataa ttgtggatgg
     1741 attccataga tataggatta tgatagatta tttagatatt tatgagagag aaaaggggaa
     1801 aatacctgtt gttattatag acaaaccatt agattgtaga atggcaagta caatacgcca
     1861 taatcgtgca agaggttcac atgatgtaga tttaatgagt aatattatac gagagcttca
     1921 tgagattgga agaagtgatg cttggatatc taaacattta ggtatggata aggatgaaat
     1981 tttaagattg aaacaaataa ccggtcttac gtacttgttt cgagatgtag aatttgggaa
     2041 agcatggatt ccagaagaaa ctatttataa tgaagaataa gtatcgtgga ttaagctttt
     2101 tatattcaat aaggaaacta gataagctta tttaaatcaa tagacaataa aagaaagatg
     2161 tactatctta aattaaaaaa gatagtacat ctttttaatt ataaaagagg tagatatatt
     2221 aacccaatct taaattttct attttttctt tttctatatt atttaaaatt tcagttaagt
     2281 tatttaaatc gtattcgacc cctaaataag taaatttaaa aatagtagtt gacataccat
     2341 ctgagaactg gtcatgaata tttgaaatta ttaggtcata tgtatgtaag tttttttcag
     2401 aaagaacttc agcttttgca ttaaactttt tacttaacaa attattgaag tctttaagta
     2461 gaactttaaa tgctaaattt tctgttttag ataagactcc tatttttatg gtatatttac
     2521 tttcctcatc tatatatgat aagtttaaga ctattaataa cttgaagtaa tcaaaatcta
     2581 aattatctaa tttgaaagat aattttatgt ctttaaataa atcaactata aagtaatcct
     2641 ttaaaaagct taaatggcta ttcaaataga aatctctagt attttcatca atagaataaa
     2701 atataccttt aaaataaaat tgcttattgc aataagacaa aatattgctc ttaacagtct
     2761 tatcaaattc aaaatcttta tttaaaaata aagacttcat tcttgataaa ataaaattat
     2821 gtacattaaa ggggagtgta atatctactg tattttcaaa agccaaaaca ccaaaagttt
     2881 ttaaaaagca agctgttgca taagctaaat actttttata atgtggaaat tttgaataaa
     2941 ataattcaag gtttttgaaa gttatatcat ttttaaaact gtctaagttt gacagagaaa
     3001 atttatctga aaaattttct ttagtaaatt tatctctgtt ctttataata atttttgacc
     3061 aaatatatat acttaatttt tgagcataag taaagtctat agacatagaa tcctctaaaa
     3121 acttgacaat atctttatca tttaatccag gtgtaatttc tttatttacg ccactatttc
     3181 taaataaaga ataataaaaa taggcaaatt gaagaggaga tccttttaaa tcaaattttt
     3241 taattcttaa ttcaaaggaa tttaataatt tgttgcactc attaattttt ctattaagag
     3301 ttgataaact ta
//
LOCUS       DAGSWI010000242         3262 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.247, whole genome shotgun sequence.
ACCESSION   DAGSWI010000242 DAGSWI010000000
VERSION     DAGSWI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.247"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            52..750
                     /locus_tag="MJF80_003804"
     CDS             52..750
                     /locus_tag="MJF80_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282404.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            928..1818
                     /locus_tag="MJF80_003805"
     CDS             928..1818
                     /locus_tag="MJF80_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282405.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLEKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGVSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2218..3174)
                     /locus_tag="MJF80_003806"
     CDS             complement(2218..3174)
                     /locus_tag="MJF80_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBZ0282406.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIQFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
BASE COUNT         1273 a          375 c          489 g         1125 t
ORIGIN      
        1 aagctaagtt ggtttttata aacataaatt tattttagag aggtgaaata aatgaaaata
       61 tctagtcaat atagaagtca atattcattt agatatgaaa gtaatataaa taatacaaga
      121 ataaatgaaa gtatggttaa gaaaaatgaa actgtaggaa aagacactta tttatctaat
      181 attatgaaac aaaagcaaga acttaatgat agaattagag atttaaaata tagacaagag
      241 gtttatacta aaaaaataaa tgacgcaatt aagaacttat gtaaatcaga aataagagaa
      301 acaactaata acttttctaa tatagaaata ggtattaaaa atagcattat agaagataaa
      361 aataaaagta caatgttaga tgaaaattca acttatctaa atacaaatga tgaaaaagaa
      421 tctttaatca ctaaagagtc taatgaaaaa attgaagaag aaatattaaa tgatgaaaaa
      481 ttagaagagt tagaacaaaa aaaggattat aaagaggatt ctaataaaaa agagaaagta
      541 tctgaggact tatctttagt aggtaaaact cgtgaagagc ttgaaaatat gcttaaaaat
      601 tttataaatt taacacaaga agaaataatg aaacttgagt cgagaataga aaagttagat
      661 aaagatgctg aagaatacaa acaaaattca aagactaata tatttgataa aacagatgaa
      721 caaaaaaaac atataaatgt actgatttaa acaatatgtt ttaaaaaaat aagtttatta
      781 tataatgaaa atataaaggt acaatttcaa tacacttcta ttaaaccaat aatttatgaa
      841 tcagacttaa tttcaattta ctaataacca tatatttatg agttaataat tttatgtttt
      901 aaaaagaatt aaataagggg gagtagtatg aagaagaata tattaactac tatgattacc
      961 atgattatta tattgatgat gagtatgaca gtaatggcag agccatatca agaagatgaa
     1021 acatctagct tggaaaaaca gagtgcaaat gaacgtaatc agactaatat aggcacaatg
     1081 gatggcagag tgcaaacaaa tgcaataagt aaaggtgcta attatgaccc taaatatcct
     1141 cttaaaggaa tgttaacaca acttggtctt aatacaacag gtaaagaggg atgggattta
     1201 ctctatggag gaaatcccaa tggagttgag cttcgagcag atggtaaata ttattatata
     1261 aataagggta tggaagggct aggattttat gcggcagcat tgtcaggtgt ctcatatcag
     1321 agaataggct ttggaaaact tgaccctata gaagtaatag ctgatggaaa ttggatttct
     1381 tacaaacaag ctcaagatac acttacagta atcaggaatt ttttaaatag ttttgactgg
     1441 agaaatgcat ctgagatgga aagagctaat agagctgcaa aattggtaac cgaagcaaaa
     1501 tatgcagact ctaaatattg taacatagtc tatggtaatc tggttgataa acgtggtgta
     1561 tgtggaagtt ttgcaagtag ctttcattta ttaacaaggt taatgggaat ggattcacta
     1621 tctatattaa atccatcatt aaatcatgct tggaactata tacaaattga tgggaaatgg
     1681 tataggtttg atgggtctga aatatcagct tttggtggat cattagactt tgattaccgt
     1741 aaattaaagg atgcaacaag agagatgact acttactatg atgctaaggc attgtctata
     1801 ttaggattta atcaataaaa atagggaagg cttcttatgg agcctttttt attgatttaa
     1861 aatttaataa ggaattttat atgaaaattt aatcaataaa agcaaaaaag gctctttata
     1921 aagcctttct aaatgttttt ttaagtaaaa ttttttatat caacatcagt tttaatcaaa
     1981 aaatataaca ttttggattt acttttaaaa ataaaattat taaaatgtaa tattaatcat
     2041 gttaataatg tttttagttt attatattct ataatagtgg tcatgttgac tattgtagtt
     2101 ttattaaaaa tagttggttt atataaatat atgcaaagat agcttgttgt ttactttacc
     2161 tattagtata taggacaaga tactctaata aatttgccta ttttaatatc ttttacacta
     2221 aaaccaacta aagtttcctt tattatttcg ttttgaataa tacaaagcat aatccgattg
     2281 tttgtataaa gactggaaag tatcagagtt attttctaat ttagatatac ctatagaaca
     2341 agaattactc tctaggcata attctaaaac tttctttgct ttattttcaa cattctcttt
     2401 tgattgatta ctatattcaa aaacagcaaa ttcatctcca ccaagtcttc caataatagc
     2461 atcatttacg aatactttag tcaaaatatt tgaaaattgt attagtattt catctcctcg
     2521 aacatgacca aagatatcat ttacctgttt aaaattatct aaatcaaaga tgtaaaagta
     2581 tccatgtgaa acagttttaa gcatttcttt tatgtgtact tcaaaaaaat gtctattgta
     2641 tagtccagtc ataaggtctt tttctgattt tagtttaaat tcatttgcca ttgctagtgc
     2701 ttcttgtgct ttttcagtta ttgttttagg gtaatactca tctttcattt ggtcaaagtt
     2761 tggaatagaa tggtgaacat ggataagttt aatcatatca tctgtaactc tgtataccat
     2821 agtaaatcga gtatccattt ctacatagat atctttttct aggtaatctt tttctattac
     2881 ccaaagagta ccatatacta taaaagcttc gtctgtgact ttggtacatg aataccactc
     2941 atctataatc tcaaagtcta tttgctctga ttctgtttgg tctttttcaa gacctataat
     3001 aaattcttct aatgtttcgt aaaattcatg ttttcctgtt ccaattaatg caaggttgtc
     3061 atctaataag gaaataataa atttaaggtt ttcttcttct ggatttaaaa tataaatatg
     3121 ccaaattttt tttgataaat ctcttaattg tttttgtatt gtattatttt ccatagatta
     3181 cccccttaag atttacagtt tatattttaa tgtattataa gttgaactct tattacaata
     3241 ttattataat atagaactaa ag
//
LOCUS       DAGSWI010000243         3254 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.248, whole genome shotgun sequence.
ACCESSION   DAGSWI010000243 DAGSWI010000000
VERSION     DAGSWI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.248"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(51..896)
                     /locus_tag="MJF80_003807"
     CDS             complement(51..896)
                     /locus_tag="MJF80_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBZ0282407.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            complement(927..3161)
                     /locus_tag="MJF80_003808"
     CDS             complement(927..3161)
                     /locus_tag="MJF80_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBZ0282408.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQYIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPNDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
BASE COUNT         1135 a          457 c          358 g         1304 t
ORIGIN      
        1 acaagtttaa aaaaagaaag ataattttaa tttattttaa aataacaata ttaataatca
       61 tttttatcaa attttttctt gtttttctcc ttgtttttat acattttttt atccaattca
      121 actattgtca attttaaatt tctttttact gtatcaacac cataactaaa tgaaagatac
      181 ttatacttag agaatacttt taaaaaattt tctcttaagg cttctagctg tgaataaaca
      241 taatttttgt caacatcact agagaatata acaataaatt catctcctcc taatcttata
      301 atagaagtag aatttattaa ttctttattt aaaagtgaaa caaaatcaca taaaatcttg
      361 tctcctacat tatgaccttc atagtcattt atcattttga aattatctaa gtcaattagt
      421 acaatataat ctcctataaa tatgtcaaat ctatcattta aatattttct attatatgct
      481 ccagttaatg ggtcagtaat tgagtagtga agatttctct tattttcttc atagagtttt
      541 gttatatcat tggccatagt aattatgtag gttttttcat caacatctac tttatattct
      601 aaaacattcc aatagcttgt agtatctttt ttaaactcta aaatccaatt tgaaatttta
      661 tcataaggat aatttttttc ccaatctaat tgaatattgt gagaatccgt aaaaaattta
      721 gcaatattat cagggagttt cttagctatt tctatattag tcattccaag tggattatct
      781 tgcatataac ttttaattaa attttttaaa ttatcattta taaatataat tctgtagtta
      841 gaatctctta taattagaaa tcctggaaaa gttcttaaaa aaatttcttt aaacatatta
      901 atttctccaa aataaaatac tttgtactag atttttgtta aattttttat tttatttaca
      961 tctacataca taccctgggc aatcgacctt ccatcaattg cagaaaggtt acattgtgta
     1021 tacataactg tgacaatttg accatctcgt tttatgaatc tatataaaaa tggttcaatt
     1081 ttatttgtgt caatcatatc caaacttttt ttataaacaa agtctaaatc atttggatga
     1141 acaattgctc ttaaatttcc gcccatctca ccaaaaaact gttcttttgt ataacctata
     1201 atatcaaaca tttcttcatt catataagta ataatcatat caccaaataa atcgtagcga
     1261 attataccta caggcaacat tgaataaaat tgtgataaaa tattactttc atcaagatgt
     1321 gtatttgtca ttttaggtat tgaatatgat tcaaattcat ttatatagcc agcagtttta
     1381 attaaatcca aatgtaattt tgctaagagt aatttatttt ctgaaattat acaatttgca
     1441 ctaaagtaga aattgctata tttttgatgt tcagaagctt catctgataa aacggcttct
     1501 ccactaatta ataatgaatc tgattcagta ttatgtgggg ttatactttt gtaaagaata
     1561 gaaaattttt tacctttaat atgtttttca aaatgttcta ttactttagg aaaagatgta
     1621 atttcagttt tgttgtaaaa atcaaaccat tgaatgtctt cagtgacatt agctcttagt
     1681 tttttcatat cttgcataat aaaaaatcca tcaaatatct tctctattaa agattttcct
     1741 atatcagaat attttataaa cattttctct gaattattct ctttagatag taatttttca
     1801 aattctttgc actccatggg ttttgcataa taataacctt gggcgatatc acaatttata
     1861 cttttaataa attctacatg ttgttctgtt tcaacacctt cacatactgt aagcatccca
     1921 agagctcttg caatgtttat tatagagtac aaaatggttg tatttttaaa atcatttaaa
     1981 ttatgagata aaaatatcct atcaattttg attatatcaa aaggtagatt ttgcaacata
     2041 ccaaaagatg aatatccaga accaaaatcg tccattgata cagaaaaacc ataagaacga
     2101 agttcagcta gttgttgaat aattgtattt gtattttcta ttaatatatt ttctgttatt
     2161 tcaatgctaa aatttgatgt agagacgctg tacttttcag taataccttt tagcatactt
     2221 ggaaaattag ggttaaaaaa atgaagacga gaaaagttac aacaaattgg aacaaaaaca
     2281 tcattgttat caatccatct tcttatattt ttacagttct cttcaaataa atgcaaatct
     2341 aattccgtta tcagaccaaa ctgttcaaat aaaggaataa atttatctgg agtaagaagt
     2401 cctttaatag ggtgtctcca tcttgcaagt gcctcacatc caactatagt attatttttt
     2461 atattataaa gtggttggta aaatacttca atttgtttag tttgtagaac atgtgatatt
     2521 tctgataaga actccttatc attttctaat ttattgtaaa aactattatc tttaaaatca
     2581 aatagaatgt atctattttt accttcactt ttagcctttt ttaaagcact atatacttgg
     2641 ttgaaaagca tcaaagtagt catgtttggt tcaccattcc agatagtgct gccaatacta
     2701 aatgtaacat tggagtatat ttctttttca ttaggaagtt ttatttttaa ttctcgggcg
     2761 ctctctataa tagttgcacc catattattt aactctttat ttgatacatt tggaagtaca
     2821 attaaaaaac cttctccttt taaataagcg actattccct ttgattctgg aataaagtgc
     2881 aaaaggtttt gagttagagt aattaataaa ttttctccga ctagatatcc atatatatca
     2941 ttaaataatt taaagttatc gatatttaat aacatgattc ctatatgtat atttttcgaa
     3001 atacagtcat caatatattg gtacataaat tctctgtttg gtattttaga aaaaaaatct
     3061 atttcattta aggtttttaa tttaaaattt ctactatcta attccttaga taatacttct
     3121 acctttttat ttaacatttt tatataatta tgattttgca tggtattcct ccataagata
     3181 ctttaaattt tgatgacaac tgtatttatg ctagaagtat ttaaatgtta atttgtaaat
     3241 aatttaaatg ctaa
//
LOCUS       DAGSWI010000244         3238 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.249, whole genome shotgun sequence.
ACCESSION   DAGSWI010000244 DAGSWI010000000
VERSION     DAGSWI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.249"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            179..1621
                     /locus_tag="MJF80_003809"
     CDS             179..1621
                     /locus_tag="MJF80_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBZ0282409.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1663..2016
                     /locus_tag="MJF80_003810"
     CDS             1663..2016
                     /locus_tag="MJF80_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282410.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2207..3028
                     /locus_tag="MJF80_003811"
     CDS             2207..3028
                     /locus_tag="MJF80_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBZ0282411.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         1250 a          326 c          561 g         1101 t
ORIGIN      
        1 attgtaacta aaatgtaaat ttttccttta atctatacaa tattatggaa actttttgta
       61 atttatgata taataacaaa tatataatta ttaaattata ataaatgact atagcgtaga
      121 tgtaatacta tgttatagtc atttttgaat taaacttgat atatcaaggg ggaagaatat
      181 gagaacaatt agaagagttt tagcattagg tttaacatta gctatatttc taataaatgt
      241 acctaatgta gatgcattaa catcagatac aattaaaggt aacaatatat atgagacggc
      301 tggattaata gcagataaaa agagttatga tacagcaatt atggtaaata tggataattc
      361 tatagcagat ggtctttcag caagtggtct tgcaggtgct gttgatgctc caattttgct
      421 tgcacaaaaa aataagatac caaatgaaac aaaacaaaga ttaaaaaatg ttaaaaagat
      481 atacataata ggtaaagaat tatcaataag taagtcagta gaaactgaac taaaaaacac
      541 aggggcacag gtaactagat tgggtggaga tgacagaata aagactagtt atagtgttgc
      601 taaagaggta aatggtatca aaaaagttga tgaagtgata ttaactaatg catataaagg
      661 agaagcagat acaataagtg ctgcacccgt atcagtaaga gatatagctc ctatagtact
      721 tacagatgga aagagtgtgc ctttttcaac aagcaatgta aaaacatatg cagtaggtgg
      781 gagtatatca atgagcacta gtttagttaa taaaactaat gctaaaagac ttggtggttc
      841 agatagatat gatacaaata agaaagttat aaaagagttt tatccagatg catcagaatt
      901 ttatttgagt gatggatatg atttagtaaa tgcacttaca ggttctacaa ttgctaagga
      961 aaatccaatt gtattggtgt cagaaagtag tgataagtct atattagcag gagcagataa
     1021 gattactaga ttaggttcaa taagtgacag tgtgtataat aagtgtgttt ctgctgcaca
     1081 aaataatggc gattcgtcta caaaaggtga gtcacctatg aaaaatgaga caagcatatt
     1141 aggtcagcca acagctagtt tagaagcgtg cttaaaatgg gcaaaatcta aaaaagctaa
     1201 tgatttattt atagagttaa taccaatatt atatgatact gctgttcagg aaggtgttaa
     1261 tcctgttttg gcagtagctc aatctgcaaa agaaactggt ttttgtaatt ttggtggagt
     1321 attagatgca tcatttaaga atccttgtgg acttaaaact tctgtaggtg gttctgatac
     1381 tgataagaat gcccattcaa gatttgatac ttgggaagaa ggaatattag ctcaaattca
     1441 acatttatgc ttatatgcag gacaagatgg ttacccactt tcaaatccag tagaccctag
     1501 acatgaaaaa tctttatttg gtaaggctaa aacagttgaa agtttatcta ataattgggc
     1561 tggaggtcaa tatggacaag atttagtaag aatgatggga gaaattgagg caacaaaata
     1621 attgaagatt atcaaatatc caataaataa ggattgatta ttatgaaaaa gagtaatatt
     1681 tttagtatca tatttcctac agtcataatg gtgctaatgt ttttaacttt tgctacagat
     1741 atatttaata ttccagatat tcaatctaaa agtatttttg ttgtgggaat ggttttgatt
     1801 tttccaattt cttttttgat acaagggata atttgtgtat taaataagac taattggatt
     1861 ttatctctta ttgtttcttt agtaacttat atagtattaa tgtatatatt tttaaatgat
     1921 tctgcatata tatatgtttt actttataca gctttttata tgattggaca tataacagct
     1981 aaactctatt acaaaattaa gacttttaaa aattaaattg aagtatataa aagtaacatt
     2041 aggagaagta tccttaatga ttttctaaaa tcagaaatag gtgcttctct tttttgtaaa
     2101 aataaaagta tgtaaataaa atttaaccat tataattttc ttttaacaaa tgtatacaat
     2161 aaacttgttg tataattaag atagataaaa taaagggagg gataatatga atttaataga
     2221 taatcatatt catactaatt tttcaagtga tggaaaggat tccatggaag atacaataaa
     2281 aaaagctata agtattggtg ttagatattt gacttttaca gaccatttag aacatgatga
     2341 agatagagga ttttgcataa attataatga ctatgttcca gtatttaata agtttaaaga
     2401 aaaatataaa aaggacatag agttattact aggtgttgaa gtgggttaca gaaaacatct
     2461 aaaaaatgaa atagaagaaa taataaattc taatcctttt gattttgtat tgtgctcaac
     2521 tcatacaatt gacaatgtac ctgttccatc aaaggcgtat tttaaaggtc ttagcaaaga
     2581 agatgcgtat tataaatatt ttaatagcat acttgaaact aatcgtgaat ttggagatta
     2641 caatatatat ggtcatttgg attatatatc taggtatgga atatattcag ataatagagt
     2701 tatatacaat gactttaaag atgttattga tgaggtctta aagtcaataa tcaataatgg
     2761 gagtggtata gagctaaata cttctggtta tagatatgga ctaaatgcta tacatccaaa
     2821 tgaagatatt ctaaaaagat ataaagaact tggaggattt atagttactg taggttctga
     2881 ttctcatagg gtagaagata tatgcaaaga ttttgatgtg gcctatgata tgcttaagta
     2941 tcttgacttt aaatatgtat ctttatttaa agaaagagaa acctattttg taaatataga
     3001 aaaagttaaa tctaacaata tagcttagag tgtgttttga agtaaattaa tagatagtta
     3061 aaaagttaat agttatagta ttgtatcctc tagaaaaata ctagaggttt ttattttgct
     3121 aaaataacta aatagaatta aatttaaata gtatagctta ttttaaattg aatagtttat
     3181 tgttgatata ttttactatt atatattttt attgtgatat aatagtaaaa tatattaa
//
LOCUS       DAGSWI010000245         3196 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.250, whole genome shotgun sequence.
ACCESSION   DAGSWI010000245 DAGSWI010000000
VERSION     DAGSWI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.250"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..701)
                     /locus_tag="MJF80_003812"
     CDS             complement(<1..701)
                     /locus_tag="MJF80_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBZ0282412.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGILVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDIAGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVKGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDII"
     gene            complement(741..1088)
                     /locus_tag="MJF80_003813"
     CDS             complement(741..1088)
                     /locus_tag="MJF80_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282413.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1306..2262)
                     /locus_tag="MJF80_003814"
     CDS             complement(1306..2262)
                     /locus_tag="MJF80_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBZ0282414.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2705..3073
                     /locus_tag="MJF80_003815"
     CDS             2705..3073
                     /locus_tag="MJF80_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBZ0282415.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         1153 a          424 c          403 g         1216 t
ORIGIN      
        1 attattatat cttctggagt tctatcaaat ttgagttcaa aattttcatc ttcaaagtag
       61 ggaatataat ataaaggtgt tttgagagca ggtaatttat tatataattc ttcaagaata
      121 tcgatatttt taggggagtt aaagaaaaag catttacata cattcattaa atccacagca
      181 tctgaaagtg agttatgttt aatatctcct tttacagaaa aaattttttt catattcagt
      241 aaagatattt gcttagataa gattctatta ttgtatgtaa tatgttgaga tataaaaggt
      301 tgaatgtcaa tcatcatatt aaataactct aagcatttac ctgtatattt atacactcta
      361 aatgaagtta gaaggccagc tatatcaaaa tcaccccaat tatatattgt aacatcatca
      421 aatttacaaa accatttata aaaatcatcc ataacatata aaagattagg agcattattg
      481 atatctagtt ggttcaattt ggttaaattt ttacatttat ttgaaagtat tttattgtat
      541 tttggcttaa ctaaagagta ataattatct attgtattaa attcgttatc aactaaaatt
      601 ccaccaatag aaattaattc cgcaccatta tattcttttg gtttattttt attatcatct
      661 agtatattaa attcaaagtc tataaataac ttgtatttca taaatctaaa gcacatcctc
      721 aggtaaatta tttattgagc ctaagcatca taagaatcaa tagatataat gactctaaac
      781 atcaaaggtt cattacaact tttatttatt tcataattta attcaaatag atcttttaag
      841 atataattta taagtattct ttcccaaccc atttttaaat taactttaga gtctattaat
      901 atattttcta tctttttaaa tatttctata tttgctggta ttatactgag ctcaatatta
      961 cttttaaacg ggttagactg taaatctgtt ataagtatat tttttaattc ctctgtaagt
     1021 tcagaaatta aactatcacc tatgtatttt tttatgattt cctctctaat ttcttttaaa
     1081 tttagcaata ttagtacctg ctacttacat agtagctttt cctttccttt taatactatt
     1141 attcattttt gaatcttatt tagatatatt ttaagattaa aagtctttag ctatataatt
     1201 ttttacccat ttttaactta tattaacata ttacaggctt atatctccac cctgtttaca
     1261 ttgaggatgg agtatattgc ctattttgat aaaaatgata ggttgttatt tgcctattcc
     1321 tttttctgat aatttttcta acatatcttt ggtcatttta tctaaatcat aactaaactt
     1381 aaagttccat tcttttactg cactactact gtctagagag ttaggccaag aatcagctat
     1441 actttgtctt acagggtcta catcatattc tatcacaaaa tcaggaatat atttttttat
     1501 agaatcagct atttcttcag gagaaaagct catagcagta atattaaaag catttctatg
     1561 gatgagttta tcagctggag cttcgattaa atccactatt gattgaagtg catcaggcat
     1621 atacatcata tccatttttg taccttctgc tatgtaagat ttatatctct tattttttaa
     1681 tgcctcataa tatatatcaa ctgcataatc tgtagttcct ccaccaggag gcgtaacata
     1741 agaaataaga cctggaaatc taacacctct agtgtctact ccaaattttg agtgataata
     1801 atcacatagt aattctccag aaactttagt cacaccatac atagtattag gtctttgtaa
     1861 tgtatcttga ggagtcatat tttttggaga attttcacca aaagcagcaa ttgaactagg
     1921 tgtaaaaaat ttacaatcca attctttggc tatttcaaga ccatttatta atccattcat
     1981 atttaagttc caagcttcta gaggtttact ttcagcaact gcagaaagta aagatgctag
     2041 atgaattata gtatctacat catatttttt tgcaatttca aaaaaacgat tcttatctgt
     2101 aacatctaat atctcaaata taccagattc acatactgga ttaccttctt ttactctcct
     2161 tgaactagcg attacatttt gttctccata ctcatttcta agctttattg ttaattctga
     2221 acctatttgt cctaatgcac ctgttataag tatttttttc aattttacaa cctcctaagg
     2281 gtttaattta tgtagtacta cttattttta ttttagcaaa tcttttgttt aaatacaaag
     2341 aattaaagat aataatatac gacttaattg aatgaagtgc agtaagtgcg taaaaattta
     2401 aagctaatat ataatggtct attgtataac aataaaagag tagattaaat tattatatat
     2461 agattgtgtg actaagtcac agaaaataaa gctaatttaa tatataataa aagcatgtca
     2521 agtaaagctt taaattaaag taactttata taaaaagtaa tataaattga taaaattata
     2581 aatttattta tagtgaatat aggttggatt ttatagatac aaaaatagta tatatgatat
     2641 gtagaccatt agcatgttta aagtagcaaa acatataaaa taaaatttga aaggaagtat
     2701 aaaaatgaca agaccatcaa tatatcatag ccaaggatac acttgtgcag aggcattaat
     2761 aaagtcttat aatgaggaac acaacacaga tataccaatc tcaataggaa gtggtatggg
     2821 ggttggaatg aatgttggaa gtgtttgtgg agctgtaaat gctgctgttg ttataatagg
     2881 gtatttaaat gggcgaaata gtaatttaga tgaaaatata gcaaggaagt attcaagaga
     2941 gctattgaat agagtaagaa ataaatacag ctcagaaata tgtgtatctc ttaaaaaaaa
     3001 taaagtaact tgtgaagaaa taataaattt cacatatgat gctctaaatg atatgctaaa
     3061 aaatcaaaaa taattctaag aaacaagtat tttacattcg atagaatact tgttttttta
     3121 tgatattttt aataagtatt agtttattat aatttttaaa tcaattttat tatattttaa
     3181 ttgaaataat ctactt
//
LOCUS       DAGSWI010000246         3164 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.251, whole genome shotgun sequence.
ACCESSION   DAGSWI010000246 DAGSWI010000000
VERSION     DAGSWI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.251"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(390..875)
                     /locus_tag="MJF80_003816"
     CDS             complement(390..875)
                     /locus_tag="MJF80_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBZ0282416.1"
                     /translation="MTELWDLYDGERKRTGNVISRNSPIPNGLFHISVSVWIRNSKGQ
                     YLISQRHSQKTYPLYWECTGGSIISGEDSLAGAVREVKEELGIYLNPLGGKIIYQTCR
                     TKTKDFYDVWLFFKDLDIEEICLQDKEVIDVKWVDRGELVKMYNDGKLHPLIDYINIL
                     P"
     gene            complement(878..1534)
                     /locus_tag="MJF80_003817"
     CDS             complement(878..1534)
                     /locus_tag="MJF80_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBZ0282417.1"
                     /translation="MYFEVAIFDLDGTLLDSMYAWERINNSFLEKRNLVVPDSYMNDI
                     CHRSLIEAAQYTIDLFNLNEAVEDIIEEWNESALVEYSNNVKIFEPAMEYIIKLKSMG
                     VKLAIATGLSKELYFPCLKNNNVLELFDVLCSTDEVSYRKEHPDIFRLVSVKTGVDPE
                     KCIVFDDILPAIKSAKKIGMQVCGVYDKYSEKNQNEIKSIADMYILDFNSAPLPYKGV
                     "
     gene            complement(1819..2046)
                     /locus_tag="MJF80_003818"
                     /pseudo
     CDS             complement(1819..2046)
                     /locus_tag="MJF80_003818"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(2458..2616)
                     /locus_tag="MJF80_003819"
     CDS             complement(2458..2616)
                     /locus_tag="MJF80_003819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282418.1"
                     /translation="MKELKDIMVYESKKESKANNISLIYELIGKELNVDNIDEIVFRN
                     FKILLKNK"
BASE COUNT         1078 a          539 c          259 g         1288 t
ORIGIN      
        1 tactttactt atatcatatc tacaatttaa ataactctca tattttttta aaatataaat
       61 ttttttgagt ttatcatttt ataggcttaa cttatttttg ttgtttacaa tatcttcaaa
      121 tgtctaaata ctgttttatc tatattatca atatttaact ctgtaaacta tgatatatat
      181 ctaatacatc cattagctta tgttacattc accatttttt cttagcatct tattttttca
      241 cttatgatta cttctattaa tttaaatatg tcaatcttct gatcacaact tctatataaa
      301 caattattaa ataaattaat atcttaattg ctcacaatta tgtataataa cttctaaaaa
      361 gctttacttt catttattga tttttttagt tatggcaata tattgatata gtctataaga
      421 ggatgtagtt taccatcatt atacatttta actaattcac cacgatcaac ccattttaca
      481 tctataactt ctttatcctg taaacaaatc tcctcaatat ctaaatcctt aaagaataac
      541 catacatcat aaaaatcttt ggttttagtt cgacaagttt gataaattat ttttcctcct
      601 aatggattta aataaatacc tagttcctcc ttaacttcac gcacagctcc tgctaaactg
      661 tcttcgcctg aaataattga acctcctgta cattcccaat acaaaggata tgtcttttga
      721 gaatgtctct gagagatgag atactgtcct ttgctatttc taatccaaac acttacagaa
      781 atatgaaaaa gtccatttgg aataggacta tttctactta ttacattgcc tgttctcttt
      841 ctttccccat catataaatc ccatagttct gtcataatta cactccttta tatggcaatg
      901 gtgccgaatt aaaatctaat atgtacatat cagcaatact ttttatttca ttttgatttt
      961 tttctgagta cttatcatat actccgcaaa cttgcattcc aatctttttt gcacttttta
     1021 tagctggaag aatatcatca aatacaatac atttttcagg atcaactcca gtttttactg
     1081 aaacaagtct aaatatatct ggatgttcct ttctgtaaga tacttcatct gtagaacata
     1141 aaacatcaaa tagctccaat acattattat ttttaagaca tggaaaatat agctctttag
     1201 aaagtcctgt tgctatggcc aactttactc ccattgattt taatttaata atatattcca
     1261 ttgcaggctc aaaaatcttg acattattgc tatattccac gagagcactt tcattccact
     1321 cctcaataat atcttctact gcttcattta agttgaacaa atcaatagta tattgtgcag
     1381 cttctataag gctcctatga catatgtcat tcatataact atcaggaaca actaaattcc
     1441 ttttttctaa aaatgaatta tttatacgct cccacgcata catagagtca agtaatgtgc
     1501 catctaaatc aaatatcgca acttcaaaat acattttaat acatctctcc ttctagctat
     1561 aaaattttac tttttattgt tgactaatat ttaagcataa atttacatat cctgtgaaat
     1621 tgactaaact tcaagatttc tggaattact ttcgacatat taacgttata tctctacatg
     1681 caataactct ccctatcatc gctccaaaat ttaaattaaa taatacttca gaagatatag
     1741 aatctaaata ttcagttagc tcatctgcaa gaattatatc tccaagctta ttgtccttgc
     1801 taatgctact ctttattgct aaaaaatatt cttatatatt atcatgtcat taattaaagc
     1861 aaatgattga aataagtaat taattgtatt acgccttaaa attgttgttg ctctactttc
     1921 tattttagaa aattcttttc catttatctt aatctttcct tcatcacttt cttctaaaag
     1981 ttctatcata tttagaagag ttgattcttc acacccactt tgtctaacaa ttgcaataaa
     2041 ctctccatat tcttaaaaaa aataaccatc taaataactg ctaatgtacc cattactata
     2101 gctccaaatt taaaacctat aataagtata ataattaatt ctatattttc ttctacactg
     2161 atatttctaa attttatata tccattaaat atctttaagc tatttgaatt tttattataa
     2221 tatattaaat agtaaaaatg cgaatagtaa tattactact cgcacttttg ttatcctgtc
     2281 ttaaaatata cctttaattt tatataataa gacaatttga caaataaaga ggtacaattg
     2341 tatggattgt ttatagtaca taacatgctt tatttatatc atatctacaa ttcaaatatt
     2401 tatcatattt ttctttaaat ataaaaacat ttgaactgtt cattttttct atatacctta
     2461 cttatttttt aataatatct taaaatttct aaatactatt tcatctatat tatcaacatt
     2521 taattctttc cctattaact cataaatcaa tgaaatatta ttggctttac tttctttttt
     2581 actttcatag accattatat ccttaagctc tttcatggat attatacatg ctttttatag
     2641 tttttccatt tcttaattag ttaaacttac agccttaata gcaagctttc ttatatcatc
     2701 ttaatttaat aataaataca tactcgaact ctatagtaaa gcttttaaat cactagacct
     2761 acactatatt tttttgtatt tattttaaat tactattgac ttttttgttg aaaatgatta
     2821 tcattataaa taactaaata agtatatttt gccaattttt taatatgtaa tccttaacta
     2881 atttaagaac tacatattcc cagagagaag aagatttgac ctccttaaac ttcaaatctt
     2941 cttttcttat gccaaaaata aataattttc atttataata tgtttatctg tgctccccta
     3001 attattaaac accacagcca aattcctttt acaatttgtt tctaataatt tttcaaaatt
     3061 aataactttt ctcattcttt cttatttaat tatttatatt tataaaaaaa taaaccccta
     3121 aaaattaggg atttatttta aaaacagcat taaaatttta ttat
//
LOCUS       DAGSWI010000247         3146 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.252, whole genome shotgun sequence.
ACCESSION   DAGSWI010000247 DAGSWI010000000
VERSION     DAGSWI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.252"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            26..1072
                     /locus_tag="MJF80_003820"
     CDS             26..1072
                     /locus_tag="MJF80_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBZ0282419.1"
                     /translation="MEKCLLLENKLPVFMKDYFIYLKGSVAVSTRLAYLEDINFFCSY
                     LIETKELTNADCVKDITEDDFNTIKSRDINLFLGDYCSRYYKNTEKNTLIFENNNRAL
                     ARKKSSISTLFKFLYRNSQIDNNITDGFNPIKLPKPQPDAIKRLEIDEVAKMLESVET
                     GEGLTEKEKVYWRKTKLRDKAILALFVTYGLRLNELRELNISSFNFSRGEFKIYRKRG
                     KEVLMPINHTCEHVIKDYLQNERTRDELLNDEEKDALFLSLQNKRITAKAIRTLVKKY
                     TSIPLDTTRDNGYSPHKLRATAATSLIQTGFSIYDVQNLLDHDNVTTTQLYAAHKKNV
                     KRDIVKNFEWIEDD"
     gene            1176..1811
                     /gene="lexA"
                     /locus_tag="MJF80_003821"
     CDS             1176..1811
                     /gene="lexA"
                     /locus_tag="MJF80_003821"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBZ0282420.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(1958..3115)
                     /locus_tag="MJF80_003822"
     CDS             complement(1958..3115)
                     /locus_tag="MJF80_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBZ0282421.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
BASE COUNT         1139 a          459 c          453 g         1095 t
ORIGIN      
        1 tattgttatc aaagaaaatg aactcataga aaaatgtcta cttttggaaa ataaacttcc
       61 tgtatttatg aaggattatt ttatatattt aaaaggttct gttgcagttt ctacacgact
      121 tgcttattta gaagatatta attttttttg ttcatatttg attgaaacca aagaattaac
      181 aaatgcagat tgtgtaaaag atattacaga agatgatttt aatacaataa aatcaagaga
      241 tataaattta ttcttaggtg attattgcag tagatattac aaaaatacag aaaaaaacac
      301 attgattttt gaaaataaca atagagcttt agcaagaaaa aaatcatcca tctctactct
      361 atttaaattc ttatacagaa actctcagat agataataat ataactgatg gatttaatcc
      421 aataaaactt cctaaacctc agccagatgc tattaagcgc cttgagatag atgaagttgc
      481 taagatgctt gaatcagtag aaacaggtga agggcttaca gagaaagaaa aggtatattg
      541 gagaaaaact aaattacgtg acaaagctat actagcatta tttgttacat acgggcttag
      601 attaaatgag cttagagaac tcaatatctc ctcttttaat ttttcaagag gtgaatttaa
      661 aatatataga aaacgtggga aagaagtttt aatgcctata aatcatactt gtgaacatgt
      721 aataaaagat tatttgcaaa acgaaagaac aagagatgaa ctattaaatg atgaagaaaa
      781 agatgcttta tttttgtctc ttcaaaataa gagaattaca gcaaaggcta taagaacact
      841 ggtaaaaaaa tatacatcta ttccacttga tactactaga gacaatggat atagtcccca
      901 taaattaaga gcaacggctg caacttctct aatccaaaca ggattttcta tatatgatgt
      961 tcaaaactta cttgaccatg ataatgtcac aactactcag ctttatgcag ctcataagaa
     1021 aaatgtaaaa cgtgatatag tcaagaattt tgaatggatt gaggacgatt aaaaatcagg
     1081 aacaaatgtt tgcagatata aatggattat gttataatta aaaatataaa gtgtatggaa
     1141 ataaaaacaa aatatgtttg gaaatgagtg atgttatgta tttagatcta actgaaaagc
     1201 aagtcttgat attggaattt ataaagtctc aaatcatatt aaaaggttat ccacctgctg
     1261 taagagaaat atgtactgct gtagggttaa gatctacttc aactgtgcac tctcatctaa
     1321 ataaacttga aaaactggga tacataagaa aggaccctac taaaccaaga gctattgaag
     1381 ttttagaacg tagtaaagta aatgatgttt ctggagctaa tcaagaaata atagagcttc
     1441 ctttagtagg tcaaataaca gctggagaac ccattttagc tcaacaaaac atagaggaat
     1501 acattccatt ccctgccagt ttagtaaagg gcagtaacaa ttttgtatta agagtaaaag
     1561 gcgaaagtat gattaatgca ggaattttag atgaagatta cgttgtagta gacaaaaaaa
     1621 atacagcctt aaattctcag atagtagttg cacttataaa tggtgaatct gctacagtta
     1681 agaggttttt taaagaagga aatttaataa gacttcagcc agaaaatgat tttatggaac
     1741 caattatgct caatgactca gaagttgaaa ttgtaggtat tgtaactggt gtatttagag
     1801 ttattaaata gctattaaaa aatacttaaa aaaataagct attcgtatag ataaaatgtg
     1861 ttatctattg attatagctt atttttttat gcatttattt tattttttga atgatttaaa
     1921 gttcagaagc tttatagata aattggttgt ttttttgtta ttttccataa catatatcac
     1981 atgggtcatt tttcttactt tctgcaattg tgcctgataa tattttttta cttcttgaaa
     2041 gcccactaca attttttgta gagtgataac tcttaccttt tggagtaaaa tacactattt
     2101 cttctgaatt ggatgtattt tgtttatttt taaaagaatc atttttactt gtattactgt
     2161 ttgattttcc agagtttcca tcttctctaa cgcctttatt ataacttcca tctttacaac
     2221 ttgtaaatac tccatcacct gttgattcaa atattatatc tgaacactca tcacttctat
     2281 gtacttttac tcccattttt tttaatttat tcattgtctc ctgatgtgga tgaccatatg
     2341 aattaccctt gcctactaaa agaaccgcat attcaggatt tactttatct aaaaactctt
     2401 gagatgaact actacgagaa ccatgatgag ctacttttaa aaggtctact ttctcaagct
     2461 taggtagtat ttctttttcg gtaccttctt cagcatcacc tgtaaataaa actttatcat
     2521 tcccattagt atatacaagg actagagatt gttcattagt agtatctccc ccatttgtat
     2581 ttagaacttc aaaataagaa tcttctaaat agaacttatt attttcaagc ggtacacttg
     2641 gggataaacc cttatttgct aaagcattta taaagtccct ataactcttt gtttctgctg
     2701 aaccattcga tacaaaaaca ttttctatat taaagttttt gacaacagaa tcaagtccac
     2761 ctaaatggtc tgcatgagaa tgggtagcta taagatattt aatatctttt acaccttgat
     2821 tgtttaggta atctaccatt aatccctcat catcattatc tcccccatct atcaatgtaa
     2881 aagttttacc atcttgaatc aatattgcat cactatttcc agtgtcaata aaatgaattt
     2941 ttacattctt atcacttgat atactactag acaaagaatc attattattt gattgattat
     3001 ttccattatc tgatgtagta caaccagtta aataaatcga tatagatact atcaataaaa
     3061 gtatcaaagt tttaatttta gaattttttt tattttttat acttatttct atcacaaact
     3121 acgcctccat attcataata ctctag
//
LOCUS       DAGSWI010000248         3139 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.253, whole genome shotgun sequence.
ACCESSION   DAGSWI010000248 DAGSWI010000000
VERSION     DAGSWI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.253"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            392..1096
                     /gene="srtB"
                     /locus_tag="MJF80_003823"
     CDS             392..1096
                     /gene="srtB"
                     /locus_tag="MJF80_003823"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBZ0282422.1"
                     /translation="MRKFLRIFINVILVLIVLYSSQKIYYKFDDYKKADKTYSKVRIE
                     RQEKQEKLDSKNKDFKFWLKIDNTNIDYPVVQAKDNKYYLNKDFYNQESKSGSIFIDY
                     RNNPNSQNLILYGHNMRNDTMFNNLLKFKDEKFFNQKNKIRIIKDDKEYIYEAFSAYT
                     IEANYDYLIPDFPNNKAFEEYIREVKNKSLFKSNLSVSDKDNIITLSTCSYEFEDART
                     VVHAKLINNLEGNLTY"
     gene            1192..1701
                     /locus_tag="MJF80_003824"
     CDS             1192..1701
                     /locus_tag="MJF80_003824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HBZ0282423.1"
                     /translation="MNLIFNKSIKVKELNEKQEVVFYVDSFFDHMTKNIGYGLLLVIN
                     NRVIIKDLGLACMHSVNFSKNILGEIMGVIKATNIAFVNSYKEITIIHNSLGIEKWIT
                     NEWSIKDQVVMEYKKIIKNRTKYMKINFKIINTNANAYRKERYGDGHLKLIYEVRSLA
                     KFASKTSRI"
     gene            2137..>3139
                     /locus_tag="MJF80_003825"
     CDS             2137..>3139
                     /locus_tag="MJF80_003825"
                     /inference="COORDINATES: protein motif:HMM:NF019941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester domain-containing protein"
                     /protein_id="HBZ0282424.1"
                     /translation="MKVTGKIFSITFLLFLLCISPISAFANEKLYQGYFENIKISGDE
                     PSVLLRITDGKGKDGKDNVVYCYDKNSQYPSALESADKTYYKRIESYLDSTDELTDKY
                     GKDKKERIAAVLNAGYPNDALGCMKKYGVSENDAMYMTQILIWDITLGTDVDYVASGR
                     ITESMAKYANEILNISKVIKFEQGKLVLEGKLEFTKKEKDWRTEKLYTTGNRGEFWFK
                     DIPSGFEIIDWNTDKKIDGNLSVGQEFYIKSSSEPSKNTRFNLEYKYNTVKFYFYKCV
                     SKNTHNNKKEYQNLIRLEPINKNNQISLEIKINGNFNEVITGGSGDKTWTEDTATEGM
                     "
BASE COUNT         1372 a          253 c          481 g         1033 t
ORIGIN      
        1 atttttcaac taatattttc aaaaaattat atatataaat aaatttttga ataatatttt
       61 ttataaaata tcattatatt aaatatgaaa agatagtgag ttttgacaaa gaaaataatg
      121 ataaattaaa gacaagctat gataaaacag taaaaatata aaatttattt agataacaag
      181 aatagaaaaa ctatcgattt tacaaaaaaa cctatcaaaa ctacaaaaaa tattgaatgg
      241 gatcttgtga atttatacaa tgttataaaa atataaggta aagcgtttaa taagagtgta
      301 tatatctaat ttagtaggaa taaaaaaata aatttgattt taacaatcaa tttttatatt
      361 taacaaagta gttaatatat ggagtggtat tttgagaaag tttttaagga tttttataaa
      421 tgtaatttta gtattaatcg ttttatatag cagtcaaaaa atatattaca aatttgatga
      481 ttataaaaaa gcagataaaa cgtattcaaa agtacgcata gaacggcagg aaaagcaaga
      541 aaaactagat agtaaaaata aagattttaa gttttggtta aaaatagata acaccaatat
      601 agactatcct gttgttcaag caaaagataa taagtattac cttaataagg atttctataa
      661 tcaagaaagc aagtctggat ctatatttat tgattataga aataatccta attcacaaaa
      721 tttgatatta tatggacata atatgagaaa tgatactatg ttcaataatt tattaaaatt
      781 taaggatgaa aaatttttca atcaaaaaaa taaaatacga attataaagg atgataaaga
      841 atacatatat gaagcatttt cagcgtatac aatagaagct aattatgatt acttaatacc
      901 agactttcct aataataaag catttgaaga atatataaga gaggtaaaaa ataagtcttt
      961 atttaaatca aatttaagtg taagtgataa ggataatata ataacactat ccacttgtag
     1021 ttatgaattt gaagatgctc gaactgttgt acatgcaaaa ctcataaaca atttagaagg
     1081 gaatttaacg tattaaacaa gtagaagtta attaaatatt tagcatatat aagtttttga
     1141 gttttatatt aaatttaata agagtttgat gttaatagga gaaaaataaa tatgaattta
     1201 atatttaata aaagtataaa agttaaagaa ttaaatgaaa aacaagaggt agtattttat
     1261 gtagatagtt tttttgacca tatgactaaa aacatagggt atggtttact attagttata
     1321 aataatagag tcataattaa agacttagga ttggcttgta tgcatagtgt gaatttttca
     1381 aagaatattt taggggagat aatgggagtt ataaaagcaa ccaatatagc atttgttaat
     1441 agttataaag aaataacaat aattcataat agtttaggaa ttgaaaagtg gattacaaat
     1501 gaatggagta ttaaagatca agtagttatg gaatataaaa aaattataaa aaatagaact
     1561 aagtatatga aaattaactt taaaattatt aatactaatg ctaatgccta tagaaaagaa
     1621 aggtatgggg acgggcattt aaaattgatt tatgaggtta ggagtttagc taagtttgcc
     1681 tctaaaacaa gcagaatata gttataagaa atgtaattat atatcaacat ttaattaaca
     1741 aatgttttat aataaactat ttaggatttt accctatagt ttgcttatat ataaataaat
     1801 tcatgcgtaa aaagataagt ttttagatga atagcgtgtt tcaaatattg tttgaaatac
     1861 gctatttttt atagatacat taatatttta tagaattatt agtcagtaga ttattaagtt
     1921 taatagcttc taattattac tatagaattt aaaattgctt catatatgaa gtaaatatat
     1981 aagaattatt tatcaatttt acataaaaaa taatcgaaac tacaaattgc attgaaactt
     2041 aatactaaaa agtttaattt attaaatagc ttttatattt atatttaaat ataaagttac
     2101 aatagatttc ttttagaaaa tttaaggagg gcaagattga aagttacagg taaaattttt
     2161 agtattactt ttttattatt tctactatgt atcagtccaa taagtgcatt tgcaaatgaa
     2221 aaattatatc aagggtattt tgaaaatata aaaatatcag gtgatgaacc atcagtttta
     2281 ctaagaataa cagatggaaa aggaaaagat ggaaaggata atgttgtata ctgttatgat
     2341 aaaaatagtc aatatccaag tgccttagag agtgcagata agacatatta caaaagaatt
     2401 gaaagttatc ttgactctac tgatgaactt acagataagt atggaaaaga taagaaagag
     2461 agaatagcag cagtattaaa tgctggttat ccaaatgatg ctttgggatg tatgaaaaag
     2521 tatggagtta gcgaaaatga tgctatgtat atgacacaaa ttctgatttg ggatataacg
     2581 cttggcacag atgttgatta cgttgctagt ggtagaataa cagaaagtat ggcgaaatat
     2641 gctaatgaga tactaaatat atctaaagta attaaatttg agcaaggaaa attagtatta
     2701 gaaggaaagt tagagtttac gaaaaaagag aaagattggc gtacagaaaa attgtatact
     2761 actggaaata gaggagaatt ttggtttaaa gatattccta gtggatttga aattattgat
     2821 tggaatacag ataaaaaaat agatggaaac ttatcagtag gacaagaatt ctatattaaa
     2881 tctagtagtg aaccttcaaa aaatacaagg tttaacttgg aatataaata taacacagta
     2941 aagttttatt tttataaatg tgtatcaaag aatacacata acaataaaaa agaatatcaa
     3001 aatttaattc gacttgagcc aataaataaa aacaaccaga tttcattaga gataaaaatt
     3061 aatggaaatt ttaatgaagt tataactggt ggttctggag ataaaacatg gacagaagat
     3121 acagcaacag aaggaatga
//
LOCUS       DAGSWI010000249         3129 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.254, whole genome shotgun sequence.
ACCESSION   DAGSWI010000249 DAGSWI010000000
VERSION     DAGSWI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.254"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            296..2431
                     /gene="rnr"
                     /locus_tag="MJF80_003826"
     CDS             296..2431
                     /gene="rnr"
                     /locus_tag="MJF80_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBZ0282425.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1381 a          280 c          564 g          904 t
ORIGIN      
        1 caatcctatt tgatggtgta taattaggtg ataaagagta atttaagcaa ttatgataaa
       61 gaataactta agtaattata atgatattta caaataattt ataaaacatc ataatttgtt
      121 tgaagttagt atgacaaagc ttacattaaa tcaaaaatat aaataagtaa ttttaacttg
      181 taataataat aaggaaatat agaaaattat gtgataagta aaaaatgttt agtctgaaga
      241 aatataaaag acaatataaa taataatata atgaaaagta aaacaggagg gaaaaatgat
      301 accaggactt aaagaaagtt tgctaggatt aataaatgaa agtgcatata atcctctaaa
      361 aaaagaagaa ttagcagaaa tatttgatat acattcatca gaaatgccaa tgttttataa
      421 ttttttaaat gaattagaag aagatggtta tatctgcttt actaaaaaag gaaaaatagt
      481 atctcccaat caaatgggat actttgttgg aaaatttgta tcacacaaaa agggttttgg
      541 atttgtagaa tctgatgttg aatatacaca ggatttattt atctcaagtg acaatataaa
      601 tggtgctatg cataatgata gagttatggc agaaatagta gtacctgcaa ctgaagaaaa
      661 gagagcagaa ggaagaatag taaaagtaat aaagagagaa ataactagaa tagtaggtac
      721 tttccaatca agtaaaactt ttggatttgt aacaccagac aacaagaaat ttactaaaga
      781 tatatatata cctaaaaaac attttagtgg agcagtagat aatgataagg tagtatgtga
      841 aattacagta tggccacaag aggatagaaa accagaaggc aaaattatag agatactagg
      901 tcaaaaaggt gaaagaggcg tagaaataga ttctataata agagaacatg gtcttccaga
      961 agaatttcca aagaaagtat tgcaagaagc agaagctgta gctgttgaga ttcctcaaga
     1021 agagataaaa agaagaagag atttaagaga tttaaacata tttactatag atggagatga
     1081 cgcaaaagac ttagatgatg ccatatcaat agaagtatta cctaatggaa acttcaaact
     1141 aggagttcac atagctgatg ttacacatta tgtaagagag aaaaataaac ttgacaagga
     1201 agccttaaaa agagctacat cagtttattt agtagataaa gtaattccaa tgttgccaaa
     1261 aactctttca aatggagtat gtagcttaaa tccatttgaa gataagctaa ctctttctat
     1321 atttatggaa atagaccata aaggaaatgt ggttaaacat gaaatatgtg aatctgtaat
     1381 aaattccaaa gctagaatga cttatacaga agtatctgat atattagaaa aggatgatga
     1441 aaaacttaaa aagacttttg aacatgttgt tgaagatttt aaaaatgctg aaattttagc
     1501 taggattctt atgtcaagaa gggaaaaaag aggagctata gacttcaatt tcccagaggc
     1561 aaagataatt ctaaatggaa aaggcgaagt tgtagatata aagccatatg aaagaagaat
     1621 atctaataag ataatagaag aatttatgct tataagtaat gagacaattg ccgaacattt
     1681 ttactggatg ggaataccat ttgtgtatag aatacatgag acaccatctt tggaaaaaat
     1741 ggaagaattg agtaagttta tatctacatt tggatatata ataaaaggtg ataaagagga
     1801 agtacatcca aaagcattac aaggtataat agagaaaata aagggcaaaa aagaagaagg
     1861 agctatcagt actataatgc ttcgttcttt aaaacaagct aagtactcac cagaatgtag
     1921 tggacatttt ggattagcag ctaaattcta ctgtcacttt acttcaccaa taagaagata
     1981 tcctgacctt caaattcata gaataataaa agaatcttta aataataaaa taagtggaaa
     2041 aagacaagaa cagcttacta ctatagtgga ttatgcatct gttcaatcat ccgagaaaga
     2101 aagaaaggca gagttagctg aaagagatgt acatgatttc tataaggctc tttatatgaa
     2161 ggataaagtt ggtcaagaat ttgagggtgt tgtatctagc gtaacttcat ttggaatatt
     2221 tgttgagtta gataatacta tagaagggtt gataagactt gcaaatatga atgatgatta
     2281 ctatatttat gatgagagtt cttatagtgt tttaggagaa agaactaaga agtcatataa
     2341 gataggagac cttgttaaga taaaggttga aagtgttaat gtagatttta aagaaattga
     2401 ctttgagata gttgaaaaaa tagaggagta gataaaacag ctaaaataag agataacaag
     2461 ttgtagtatt tatgatatgt ataaatatat agtgcacaat actctataaa ataaatgata
     2521 aaaataggta attgagaaat atctaatgga aatagttaac tgaaagataa ataattaaga
     2581 aatatttaat gaaagtagct aattgaaaaa taagtaattg agaaatgttt aatagaaaga
     2641 gctaactgaa agataagtaa ttaagaaacg tttaatagaa gtggctaatt gaaaaataag
     2701 taattaagaa atgtttaata gaagtggcta attgaaaaat aagtaattaa gaaatgctta
     2761 atagaagtgg ctaattgaaa aataagtaat tgagaaatgc ttaatagaag tggctaattg
     2821 aaaaataagt aattaagaaa tgtttaatag aagtggctaa ttgaaaaata aataattaag
     2881 aaatgtttaa tagaagtgac taattgaaaa ataagtaatt aagaaatact taatagaagt
     2941 ggctaattga aaaataagta attgagaaac atttaataga agaagctagc tgaaagataa
     3001 gtaattaaga gatatttaat gtaaacagat aatttaaaaa tacctaatta agaatagata
     3061 ttaaaaaata taatttcaat ataaaaataa gtgtatcatt taaaatttaa gatacactta
     3121 ttttttatt
//
LOCUS       DAGSWI010000250         3072 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.255, whole genome shotgun sequence.
ACCESSION   DAGSWI010000250 DAGSWI010000000
VERSION     DAGSWI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.255"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(376..1443)
                     /gene="rsgA"
                     /locus_tag="MJF80_003827"
     CDS             complement(376..1443)
                     /gene="rsgA"
                     /locus_tag="MJF80_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBZ0282426.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVYRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWKAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(1440..1841)
                     /locus_tag="MJF80_003828"
     CDS             complement(1440..1841)
                     /locus_tag="MJF80_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBZ0282427.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      2637..2733
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2787..2963
                     /locus_tag="MJF80_003829"
     CDS             2787..2963
                     /locus_tag="MJF80_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282428.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
BASE COUNT         1070 a          521 c          380 g         1101 t
ORIGIN      
        1 ttttataaat atattatttg tattttataa ttcatatatt tttactttat aaattagcaa
       61 tattgcagtt ttgctcttaa gaaacatctc tatcatacat aagcatatac tctattatgt
      121 attagtataa atatgccttt tcttatctta cataagacct ctttataaag gagactatcg
      181 taacaaatat gtatacatta tcacatgtat atatcatcat ataaaaaatc aaactttatt
      241 aaaaaaaata ataaattgtt ttaaataata ccacaaatca aatttgccat caaaatcaca
      301 ttaaagtaat aaattaatgg caaatttaat atattaatag tatatagact catataatga
      361 acaaaaaaac tacacttatc ttctaatctt cttatttatt tttgagatat ttttaaattt
      421 cttttctttt tttgctaagt attcgctttt attgtctgca aatactgctt cagttttaag
      481 cctacgataa gactcccatc ttttaggcga aagttcttga tgagcaatag ctttccagat
      541 agcacagcct ggttctgagg tgtgagtaca attacgaaaa tgacattttc cgaaataagc
      601 ttctatatct gcaaaacttt tatctaaacc ttccgaaaca tcccacattc ccaactctct
      661 catacctggt gtgtcaataa caataccacc acctgaaatt aaaaatagct ctcgtctagt
      721 cgtggcatgt cttcctttat catcatctat gcgaatttcc tttgtattta aaaattctcc
      781 acccaacaaa caattaatta atgaagattt tccaactcct gaagaaccaa gtaaggcaac
      841 agtacagcct ggtttaatat acttagaaat ctgtttataa ccatctggct ctatagatga
      901 ggtaacaact acatctacac ccaaggctat ggaattaact acttgtaatt tacgatacac
      961 attgtcacat aagtctgatt tagtaagtac tactactggg atagctccac tttcccatgt
     1021 taaagacaga tacctctcca atctatgaat gttaaaatcc ttattcagtg acatgcacaa
     1081 aaaaacaatg tcaatattag ctgcaaccac ctgttcctca tttacctttc cagcagcctt
     1141 acgcacaaac aagctttttc tatgaagcac atgatgaata atagcattac catttttatt
     1201 attgcaacga tccaacataa cgaaatcacc aactgtagga aaatcagatt gtatagtagc
     1261 ctcaaaacgc agctttcctg aaacctctgc aaacagtaca ccatttacac atacagcttg
     1321 ataaaagttc ttttcctgcg agataattcg agcaaggtat aaatcatgat attccatact
     1381 ttgagtaata aaattcctat ttagaccata gtcttgtaaa ttgatttgtt tcataccatt
     1441 cattctgttt cctccttttc caacttttca atgattcttt ctagtgggaa agggggctgt
     1501 gccagtggtt ttaatgcaat tgacattaat ttcactgaat tatcatcagc tgcaatacga
     1561 ttagaactaa gatgtccaca gcgtgtacaa cgatgaataa ttgcccactc accatttttt
     1621 ctcacccaca ctccaatagg ttccatatta gcatcacaat ctgcagcacg gtcaccagga
     1681 atgatatcta catgaagact atgtaaacaa tgtggacaat gatttctatg ttgtgtacca
     1741 gctccttcag gaactacaag acaaccacaa tttttgcaaa taaagttatc catacaggaa
     1801 ttttcctgat aataagctcg ttttctattt ttataattca tttttatttc ctccgtatcg
     1861 tgtattttct acaaaaaaag tccacaggta tttatatctg cctttttatg tggtatacat
     1921 atttacctac acaaaaacag aaaacccctg tggactagaa aatttagtat cttctatttc
     1981 aacataatta tattcaaaat aatactgtgt aaaattaata aaagtgtagc agattaaagc
     2041 ccactacacc acaatttaac aaagtaatat gtaatattat tatttgttgt ctaatgttag
     2101 ttatctataa aggcagagta agacacaaca atcaaatgta tcaaaaatac acttaaaaat
     2161 tctgattaaa cagaacccat atgcgtctcc aactatttat ttgttggtta ccattatata
     2221 taactctgac atcaggcaag acactccttc actaataatt attattgaaa ttttatcata
     2281 aaatataagt atgttcaagt ataatttaat atattcatat atttatgttt gtctcatttt
     2341 cttattctat aaaatctagt ggtattttaa aatttaatgt tcacctagta ttaataataa
     2401 aaattaactg ctattatttg ttttctcatt ttatgcctat tgctcaattt atctcactta
     2461 aattaataac aactgtcttt tcatacaaaa cctacaatca agtttttcca tatattctac
     2521 atgcatagtc tatttttttg atataaaata acgttcatat ttgtaaattt atttaatttt
     2581 caaataagta tattgaataa taagcaattc tattgtatta ttgtaattgt agaataaaac
     2641 taaatcggca aaactagaga aatttagtga cgcaaagcta tagggactaa cgcttataaa
     2701 ataaactatg agttatgtca gccagttgcc aaaaagagat attctttttg tttttatgaa
     2761 agttttttag ggggataata atatatatgt ttagagataa aatggataga tgtacacata
     2821 tgttgactgc ttatattggt agttcatatg attattgtga ttttatagat acacaactag
     2881 atgattttat actagagtac ggagaaaatg tggtagaatc ttgtttgcat caagtgatgg
     2941 tattagtaag taagtataat taaatagatt tggttttcta gagaaccctt tttagtttta
     3001 tctactttaa atagccttat gttagaaagc taattaagat agatttctag tattcataat
     3061 tttatttttt at
//
LOCUS       DAGSWI010000251         3059 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.256, whole genome shotgun sequence.
ACCESSION   DAGSWI010000251 DAGSWI010000000
VERSION     DAGSWI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.256"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..618
                     /locus_tag="MJF80_003830"
     CDS             <1..618
                     /locus_tag="MJF80_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HBZ0282429.1"
                     /translation="ADGATGPTGATGATGVTGATGPTGPTGATGATGASAIIPFASGI
                     PLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFSMPRDGTITS
                     ISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLTGILSVGSIS
                     SGIVTGLNIAATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            787..1548
                     /locus_tag="MJF80_003831"
     CDS             787..1548
                     /locus_tag="MJF80_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBZ0282430.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1857..2135
                     /locus_tag="MJF80_003832"
     CDS             1857..2135
                     /locus_tag="MJF80_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282431.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            2428..3003
                     /locus_tag="MJF80_003833"
     CDS             2428..3003
                     /locus_tag="MJF80_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBZ0282432.1"
                     /translation="MKKITWEVEYISRLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
BASE COUNT         1171 a          360 c          552 g          976 t
ORIGIN      
        1 gcagatggag caacaggtcc aacaggagca acaggggcaa caggagttac aggagcaaca
       61 ggcccaacag gcccaacagg agcaacagga gcaacaggtg ctagtgcaat aatacctttt
      121 gcatcaggta taccactatc acttacaact atagctggag gattagtagg tacacctgga
      181 tttgttggat ttggtagttc agctccagga ttaagtatag ttggtggagt aatagacctt
      241 acaaacgcag caggaacatt gactaacttt gcattttcaa tgccaagaga tggaacaata
      301 acatctattt cagcatactt cagtacaaca gcagcacttt cacttgttgg ttcaacaatt
      361 acaattacag caacacttta ccaatctact gcaccaaata actcatttac agctgtacca
      421 ggagcgacag ttacactagc tccaccactt acaggtatat tatcagttgg ttcaatttct
      481 agtggaattg tgacaggatt aaatatagca gcaacagcac aaactcgatt cttactagta
      541 tttactgcaa cagcttcagg tctttcatta gttaatactg tagcaggata tgcaagtgca
      601 ggaattgcaa taaattagat tagataagaa aaggcttcta atatcgtatt gatattagga
      661 gcttttttaa gaatgattga tgaacatagt tttaatatta attagtgcgc atattataaa
      721 atactattat ataatataaa ataataacat taaattggaa atatagaatt atctaggggt
      781 gatactatga aaaataagat tgaaatatca atgaacaact ttgtaaaaaa ttatcataaa
      841 tcaaataata taactacaaa gtggcaagaa cttttgtttg cttatgcatc tgctgaagat
      901 gatatgtttc agaagctaaa aagtgttgta ggaacatctc atgctatgcc aaaagatttt
      961 ctggaaaatg gaaaaactat aattacatat tttatacctt ttgatgaaag tgtaaataat
     1021 agtaatattg gagggtttga atgttctgaa atatgggcaa gagcgtacat agaaactaat
     1081 atgttgattt caaatttaaa tgatttctta tgtgaagaac tagagaagat aggggctaat
     1141 tcatatgcta ttcctgggtc gtataatttt gacagtgaaa aattggtaag tgactggtca
     1201 caaagacatg tagcattcat agcaggtctt ggaacttttg gtttaaataa tatgcttatt
     1261 acagaaaaag ggtgttgtgg aagaatagga agcataatca caagtttaga attagagcca
     1321 actaaaagac cagaaaatga atactgcttg tataaacata ataatagttg caaaaaatgt
     1381 gtcaataaat gcgtgaaaga agctttaaaa gaagatggat ttaataggaa taagtgttat
     1441 gaaatgtgta tgataaatga aaaaaaatac acttatatag gtggatgtga tatttgtgga
     1501 aagtgtatag ttggagttcc ttgttcagta ataaacccat caagataatg gatttaagtt
     1561 gactaataat tttaaagata gtttataatg aaaaaatttt agttatcaaa ataaatataa
     1621 tataaaaact aaataaatgt gtttaaaaaa tggataaata atttgtgttg attgacaacc
     1681 agttatactg ttgatagact tatatgtatc gctgtgatat ataaggaggg ttgagatttt
     1741 atgtttgatt taatctttat ttttgcatag caagggctat tgcaactatg aaatttaata
     1801 tataaatagc cctttgctat tcctaatgaa aaattactat ttaataggag agcaaaatga
     1861 gctataaaaa agcaatacat atattaccag aagaattact tgaattaata caggaatatg
     1921 tagatggtga atgtatctat attcctagaa aatctaataa taaaaaagaa tggggaagta
     1981 atacacctac tcgtgaagag ttgtcaatta gaaatatgca gatttacgaa gactatcaag
     2041 ttggatatga tttgcaatat ttatcggaaa agtattattt atctttgaaa agtatccaaa
     2101 gaattgtatt acaagaaaaa agaaaagata tctaaggatg agccagtata gttttataat
     2161 tatactggct cattttcttt tgataaaagt gtttgaggta tagtttcttt taagtggatg
     2221 atataattta agtaatttca ataacttata cagtagctaa caaagctggt tgtaaagtat
     2281 ctaataagaa gtagtagctt tttatatgtt tgaaattaaa atttattaaa attaggaggt
     2341 cttgttttga aaaaaatact tgattatatt ccaccttttc ataactttct attaatttag
     2401 taatcttata gaaaggaaat tgtatttatg aaaaaaatta cttgggaggt ggaatatatc
     2461 tcccggttac ctgtaattag gtattgtaaa aaatgtggaa aaaagactga atatatttgt
     2521 tcaggtttat ttagggttaa tgctcaacat aaatatttgg atatatggtt aatatacaag
     2581 tgttcaaatt gtgattcaac atggaactca actatatatt ctcgtgttaa tccaaaaagc
     2641 ttaaatccag aaatactaga acaattccat acaaacgatt ataatcttgt taagcaatat
     2701 gctatgaata tggaactttt acatagaaat ggagcagaag ttggtattcc agagtataaa
     2761 atagtaggac aagaaattaa tattaatgac cttacacaag ttcatattat tagtaaatat
     2821 ccatgtcagt taaaggtatc aacattatta cgtgagaaac taggtttatc taggaaggta
     2881 tttgacgaaa tgttggattg tgggattatt agaagtaact caggagttga tttaaagaaa
     2941 ggtaaattaa attatgaaat tatgttagat ataggcaagg agaagcaaaa gtcttcaaat
     3001 tgattagaat aactaactta atatattttc aggaacatca tttaaaaaaa tagtggctt
//
LOCUS       DAGSWI010000252         3043 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.257, whole genome shotgun sequence.
ACCESSION   DAGSWI010000252 DAGSWI010000000
VERSION     DAGSWI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.257"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            192..458
                     /gene="rpsT"
                     /locus_tag="MJF80_003834"
     CDS             192..458
                     /gene="rpsT"
                     /locus_tag="MJF80_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBZ0282433.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(645..1688)
                     /gene="holA"
                     /locus_tag="MJF80_003835"
     CDS             complement(645..1688)
                     /gene="holA"
                     /locus_tag="MJF80_003835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBZ0282434.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1642..2943)
                     /locus_tag="MJF80_003836"
     CDS             complement(1642..2943)
                     /locus_tag="MJF80_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBZ0282435.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYAFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIESPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT         1119 a          444 c          282 g         1198 t
ORIGIN      
        1 aaaacacaaa atataccaat ttaaaaacaa gatattgcaa attatttata cacaatttac
       61 agtctaaaaa atcaaaataa aaaacattat taaaatatat aaataataat ccttgatttt
      121 tagatttatt tctgttaaaa tctaatatgt tgacgatgaa tatgataaaa aaatacaggg
      181 aggtgaacac ggtggcaaat ataaaatcag ctaagaaaag aataagtgtt atcgaaaaga
      241 aaactgcttt aaatagagtt agaaaatctc aaataaaaac tgcaataaga agattcgaag
      301 atgctgttgc agctggaaac agagaggatg ctgttgctaa gttccaatac gctcaaaaaa
      361 gaatatacca agtagcttct aaaggaacta tacataagaa tgctgcagct agaaaagtag
      421 ctaaattagc tcaaaaatta aacggtatga acgcttaatt accagtttat atttcttata
      481 tataataacc cctatgtagg ggttttttat tttgtaaatt tatacaataa aaaataaata
      541 gttagacata ttatagttga ttgtatatca aacttaattt agcttacatc tactctgaac
      601 tataacatat cttgctattt attttttatt aatatataga ttttttaaat tgcttctctt
      661 ttacagtatc tactaaccaa catttcaact gctaaagtat ctcttataag acctgtcttt
      721 attttgtaat cactttctaa tatatagttt aatatttcca caattatatc atcagaaaaa
      781 tttttagttt gctttaatgc ttttcctaca acaaattgat gcatttttaa tttatcagct
      841 ataagttttg ttgaatatcc atcaagttgt aactgtctta cttgcataat tattttaaat
      901 tgtcttgcta tcatagaaaa tatacctaat acagattcgc cttcttgtat catatcattt
      961 aatattttca ttgcattaga agcattttgt tctccaatat aatcgattaa tttgaatata
     1021 tcattctcaa cttttttttg agataattta tcaattacat cattggttac attattccct
     1081 ttaccaacaa atgaacttat tttatttatt tcattttcta aatcagataa tgtcttttca
     1141 ctactcttat ctctgtatcc ttcttgttca ataaaataca tgatttgaga attatctatt
     1201 ataacatcat ttaaagcaaa agattttttt atccatttaa acaaatccat atcagaaagc
     1261 ttatcacaat caaaaacaat tccattgttt cctattttct taactaatga ttttcttttg
     1321 tctacatctc catatacaac aaaaactata gttgtggtgt ctggaatatt gtctaaatgc
     1381 tctattaagt atttctcatc actatctgta aaattctttt tctttccttt taatagttca
     1441 aagtctttta ttattactat cttcctatca tccataaatg gaagtgtttc tatagagctt
     1501 ataagctgat ttaagactat ttccttccca tctattatat ctaaattaaa atcaagcatt
     1561 ccctcgttta gactatcttt aaatgctttt attgcattct caattaaata atactctctt
     1621 ccataaaaaa gatacatttt ttcaaagttt ttctctttta cattccttat aatatccttg
     1681 taattcattc tcttgctcct ttatctcttt tccttctatg taaaatatgt aaaaaaacac
     1741 tcctatgtaa taaataacaa caatataaaa tttaggactt tctatattaa tgtatgcaaa
     1801 acttaaattg ctgatttttt ctagtaaaaa aaatatgctt tctatcaatg tactattaaa
     1861 aaaagatata attttagcta ttactacact tactttaaat acgattaaac ttgcaatact
     1921 taaatacatt attactccca caaatggtac tataattaaa tttcctataa ttgaaagcaa
     1981 tggtatacct ttaaatgcat aataaattat aggtaaagtt aatatatttg atgatattgt
     2041 caatgatacc atacttaatt ttataatact atttatttta ttatagaata ttaatataga
     2101 aagtgtagct aaaaaagaca attgaaaact tacattatat ataacatagg ggttattcat
     2161 aattaaaatt atcccaatta gagacaatgc cgatattcca tctttctttc tatcaataaa
     2221 tattgataaa taaaaaatca taacaaatgc tatagaccta attatggatg gagacgcacc
     2281 taccattata caatataaag ttataaaaat cgacagtatt aataatttat acaatttatt
     2341 tatacctctt aatagaaagg atataattac acataatatt cctgtatgaa gacctgatat
     2401 agatattaca tgactagttc cagttctagt aaaaatttct ttttgttcct cagtcaaatt
     2461 ttcaacttct gctaatagta tagaatttat aaaatctgag tttttcttat ataagtacct
     2521 ataagtttta ctaatataaa actttatttc ccctattaaa gagtaaagtt tatttttatc
     2581 aataatttta tagttattta tatacactat acctttatat ccagtacttt ttatatacct
     2641 tccataatca aaatcatcat acttcatttt atctaaactt ttgaatttac ctgtaatatt
     2701 aactttctga ccaatcttca aatttttatt ttttgtataa tcatttatta gaaaaacccc
     2761 taccttgtac tgattgtatc ttttcttttc aatttttcct ttgacaactc cttgtatagt
     2821 tattatctta tcattaagtt cttcgtcgtt ttttgtgtta tttgtaatga taaatgatat
     2881 agctaccaca aacattaata taattaataa tggtcttctt atttgaatta taatcactcc
     2941 catataaaag tatattattt agattatttt caaatagaaa atttttaaac aagataaatt
     3001 gttatctaaa caaaaaaata cgtcaaaaga tttttaaaat ctt
//
LOCUS       DAGSWI010000253         3012 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.258, whole genome shotgun sequence.
ACCESSION   DAGSWI010000253 DAGSWI010000000
VERSION     DAGSWI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.258"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(519..1838)
                     /locus_tag="MJF80_003837"
     CDS             complement(519..1838)
                     /locus_tag="MJF80_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBZ0282436.1"
                     /translation="MLNINVFWIFLPIFSTTIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADK
                     YIKDINKQIKECNTIFNTQNMILDVILSDKKSICDMNSIYFLVDINFLECNFIEMPDI
                     CSIFSNIIDNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKVI
                     TSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
     gene            complement(1832..1960)
                     /locus_tag="MJF80_003838"
     CDS             complement(1832..1960)
                     /locus_tag="MJF80_003838"
                     /inference="COORDINATES: protein motif:HMM:NF016296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282437.1"
                     /translation="MEYIQFICKNTVVINGKDIPVSKYRMANLKKKLTYVLGDVLC"
     gene            complement(2099..2809)
                     /locus_tag="MJF80_003839"
     CDS             complement(2099..2809)
                     /locus_tag="MJF80_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282438.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKNTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
BASE COUNT         1108 a          462 c          195 g         1247 t
ORIGIN      
        1 ctataaataa ttaaatcttc tacattaaaa atcatttctt cagaatctat cacaactaag
       61 tcctcctcaa attagtaaaa tatttttctt taatatttat tactaatatc aactttacta
      121 tctaattagt tatttaattg ttgtttttat gttcctagtt ataaagttta tcaaatttac
      181 attttatttt tttatactat atagaacttt tggtcaataa tgttaccata ctaaggtcgc
      241 atttgcagat aaaaataact ctatttttct aaatttattt aagatatatc atgcaaataa
      301 tataactata aaacctatca acattttaaa ttcaaacaaa tataaatcac attttttgtt
      361 ctattatttt attgagtact aaaaaggatg ttatcttatt agcatagcat tcaaccattt
      421 ttatcacctc caaatatcct atgccttaaa gatagcatct tctattttat ctttcaatac
      481 tactgctcca actgggcaat ttagagcccc aactagaatt agttcctagt taatggtata
      541 taaatagtaa ttgtaaactt gtttttatct gagtatattt ccacatttcc attatatttt
      601 tctactgaac tttttacact acttattcct attccatgta aaaaggaatc tttcttactt
      661 gtaataacct tattattttt taatacaact tcatttatct ttgtattttc acattttatt
      721 acaaataacc tatttataac agttcctctt aatttaatct ttttttgtat acttgtgtcc
      781 ttaatcttaa tacaagcttc tattgcatta tctattatat ttgaaaatat actacatata
      841 tctggcatct ctataaaatt acattctaaa aaattaatat ctactagaaa atatatacta
      901 ttcatatcac aaatagattt tttatcactt aaaataacat ctagaatcat attctgagta
      961 ttaaatatag tattacattc ttttatttgc ttatttatat ctttaatata cttatctgca
     1021 aactcatttt tcccataaac attctgaata caaatgatat gattattcat atcatgatat
     1081 agttttctta ttttcaactg atattcctgt aaatttaaat aatatttata ttgcatattc
     1141 attctttttt ttgtaatttc atattctatc tttaaactat tatcttttat tattcttcct
     1201 attatgctta ctaatgaaag atttgatagt aaaaatacag ttgatattgt aagtattatt
     1261 aaactctctt tagaatcgat aatcttatct ttaaaaataa attcaaatat tactattaca
     1321 ccaataatgt ttgctattaa tggaatagat aaatatatac aatccctctt gtttattttt
     1381 gtttttaatc tgataaactt aagcaatggt attaatacta ttagtaatga tttagatatc
     1441 attattaact ctaacttcaa caaattatcc tctaataaat cattcatatt ttttatagaa
     1501 ttaagtgtca ttactatact taatccaata ctatcaaatc caattaataa catccaatat
     1561 agcaaactta taaatacact tttccattta tccactacat aatttatttt atacaataca
     1621 tatgtaataa atatacatat aaataattta atatttatat taaactcaat tgtattcaac
     1681 atagtaatta tcataatagc aattactatg ctactattta gggttaatct attctttctt
     1741 aactcactaa attcatttaa taaaaatttt aatacaatcc attctatcgt cgtagaaaat
     1801 ataggtaaaa aaatccaaaa tacatttata tttaacataa tacatctccc aatacatatg
     1861 ttaatttttt ctttaaatta gccattctat atttactaac tggtatatct ttaccattaa
     1921 taactacagt atttttacat ataaattgaa tatactccaa attaattaaa tagctcttgt
     1981 aacattttta aaaatttatt ctttcttaaa tcactgaatt catttgataa aaattttaat
     2041 acaattcatt ctatcgccgt agaaaatata ggtaaaaaaa tccaaaatac atttatatct
     2101 aacataatac atctcccaat acatatgtta attttttctt taaatcagcc attctatatt
     2161 tactaactgg tacatcttca tcattaataa ccacagtatt tttacatata aattgaatat
     2221 actccaaatt aattaaatag ctcttatgac atctaaaaaa attatatttt tctagctcta
     2281 attctatttt atccatacta tttttagtat aataagtatc atttttagta tgtatagtca
     2341 aaaccttttt cctaacctct atatacataa tactattaat taaaatttta ttgattatac
     2401 ctttattttc tatcagcata aaattttctc ttttttttat aatatccgat atacaactca
     2461 aaatactttc atttaaatct ccaaaatcta taggctttaa taaatatcta taagccctta
     2521 ccttatagcc ttcctgtatg taatcaacta ttgaagttat aaatattatt tcagaagtat
     2581 cattagtttc tctaattatt ttagccacat ccatgcctgt tagttgaagc attttaatat
     2641 ctaaaaaaaa tatatcaata ccttctaaat ttgtttccaa taattcttct cctgaattaa
     2701 actcaaatag ttcaatttga ctagaaatac cctctaagga ctttaatata cactctctta
     2761 aaaaagctat ttgtgtaata tcatcctcac agataactat tctatacata aacacccctc
     2821 cttagacatt agatctgaaa acaaataaaa tatcaattta taaataatta tttagttata
     2881 caactaacat ggtcatcaaa tactattatt cacttaaaaa atcaccccct atttttagta
     2941 tgttaacaac aaaataagaa gatatcttag atttttaaaa acaattaata cttaatgtag
     3001 ttttgtatac ta
//
LOCUS       DAGSWI010000254         2854 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.259, whole genome shotgun sequence.
ACCESSION   DAGSWI010000254 DAGSWI010000000
VERSION     DAGSWI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.259"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            128..496
                     /locus_tag="MJF80_003840"
     CDS             128..496
                     /locus_tag="MJF80_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBZ0282439.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            499..870
                     /locus_tag="MJF80_003841"
     CDS             499..870
                     /locus_tag="MJF80_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBZ0282440.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1014..1469)
                     /locus_tag="MJF80_003842"
     CDS             complement(1014..1469)
                     /locus_tag="MJF80_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282441.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1508..2773)
                     /locus_tag="MJF80_003843"
     CDS             complement(1508..2773)
                     /locus_tag="MJF80_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBZ0282442.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVMVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1050 a          406 c          396 g         1002 t
ORIGIN      
        1 caaagttata ttagtaaaaa ttggaattat ttataatata ctccaaaatt taaaaataat
       61 ggttgaaaaa tctaattaag agtactataa tttaattaag taattactta atcaaaacga
      121 ggtacttatg gaaaacttgg ttaaaatatt taaagcattg agtgatgaaa ctagacttaa
      181 catacttata ttagtatcca agagaaatat atgtcaaaag gggatttcta aatatctagg
      241 gatttcagat tctgctgttt cacaacatat aaaaatatta aaagatgttg ggataataac
      301 aggatataaa gaaggatact atgttttata tcatattaat aaagaatcat ttaatatatg
      361 tgtagatttt ataaattcta tgttaagtaa ttctagtgaa agctttatag atgtatttga
      421 tgtaaataca atacatctag gatgcagtaa agaatgtaaa tctataaaaa aatgttgtaa
      481 gagaagggag aaataataat gaaagtatgt ataccagtag aggagaataa aggattggat
      541 agtaaaccat atggacattt tggttctgct ccaatttttg ttgtgtgtga tttagaaagt
      601 ggagaagtta aatctttaga taatggagat ttagatcatg aacatggaaa atgtcaacca
      661 ttaaaagcat tgtcagggac agctgtagat gcagtagttg ttggaggaat aggacaagga
      721 gctataatta aattaaatgg aatgggaata aaagtttaca gagcagaagg tgatactata
      781 agtgctaatt tagacttact taaaaatgga aaattagtag aatttccatc agaccatact
      841 tgctcacatg acggatgtgg acatcattaa aaaaaacagc tgatgaaata tatacttcat
      901 cagctatttt ttcttttaat aaatacgatt ttattctttt aaaaccaaaa tttagatttt
      961 tatgtttttt gtggtatcct taataatatt tcaagtttac aagtttacta cttttagatt
     1021 ttttttgtat gtttttctat atagtcaata tcatttaata tgtcattgag ttctttatta
     1081 agtgttttta aatcatcacc atctacattg tagtttaaaa atagttcctc tacattatca
     1141 atgatagaat tgacttttct agtaacttca acaaatgtct taaagttact actattttta
     1201 gcatttagaa atttttttaa atcctttgat ataaatgata ctatttcata ttcatcatta
     1261 aatagacctc taccaatagg atatggttca attctctgta aaaaatattg gtctttagca
     1321 ttaatattat aagtataaga tatttcccaa ccgtctccta aatcaaactt gataaaatat
     1381 aatgtactac ttacttttga tattcttgtg gcaccaagct gtcttaaaag aacttcaatc
     1441 gaatcacctt gaacagtatt aatattcata tatacacatc ccctttagta ttattatact
     1501 atatttttta aaattttata attaaataaa cagtagatat aataatagat ataatcataa
     1561 gtggaaaacc tattttgaaa tactccttaa aagagatagg atgaccgtgt ttattaccta
     1621 tacctgctag tacaacatta gcagatgctc ctattagtgt tccatttcct ccaagacaag
     1681 ctccaagaga tgttgcccac catagtggca ttacatcaat accttcagct tgcatagtca
     1741 aaataagagg aattagtgtt gcaacaaatg gaatattatc taagaaagaa gagactatag
     1801 ctgacaacca taatatcaac aacattgtaa atacaagatg tccttctgta agtccaatca
     1861 atgcttgagc caatttgttt attatcccaa cttctactaa tccaccaact actacaaata
     1921 atcccataaa gaataaaatt gtagaccatt caatacctgc cataatttca tctacatctt
     1981 gttttcctat taaaagcatt atagatgctc ctgtcagtgc tacaacagaa gaatctatgt
     2041 ggattgtact gtgaaacata aaaccaaaaa gtatgaatgc tattaatata aggcttttta
     2101 taagtagagg tttatcttta acagattttt tttcatctag ttttaatatt gcatttttag
     2161 ctctttcatt cacaacaagt tcttttccat aaataaatct aaagcatatt atagttatag
     2221 caagtattac cataacagca ggacctaaat ttattacaaa atccataaaa ctaagattgg
     2281 cagcactacc aatcataata tttggagggt ctcctataag agtagctgtt cctcctatat
     2341 ttgaagctaa aatctgtgtt attaaaaatg gaactggatt aagtcctaat atttgtgtaa
     2401 taactatagt cattggtcca ataagtaaga cggttgtaac attatctaat acagctgata
     2461 aaatagctgt tataatagca aaacaaatca ttatcttcca tgggtcacct ttggctttct
     2521 ttgcagtcca gatagctata tattcaaata accccgagtt tttgacaaca gctacaaata
     2581 gcatcatacc aactaataca ccaatagtat tgaaatctat atggcttaat ccttcatcaa
     2641 gtgtaagtat attgaatatt atcattaata gtgcaccacc aacagctgct acggatctgt
     2701 ttaccttttc tgtaataata gctgccataa ctattaaaaa tatacatata gctattattt
     2761 gttgaaattc cataatataa gtacctccat ttgtattatt ttgtgcctaa tgctagtact
     2821 atatatagta ttactttttt attgtttttt caat
//
LOCUS       DAGSWI010000255         2812 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.260, whole genome shotgun sequence.
ACCESSION   DAGSWI010000255 DAGSWI010000000
VERSION     DAGSWI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.260"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..986
                     /locus_tag="MJF80_003844"
     CDS             <1..986
                     /locus_tag="MJF80_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBZ0282443.1"
                     /translation="AEAQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQIT
                     EEAMKLKESKDVSILAAYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKL
                     KTFVLDEADQMLLMGFKNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVV
                     SIQKEEITLNNIKQEVVETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALA
                     IRGYNCQKLHSDIAQSKRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPE
                     TPEDYIHRIGRTGRAGEEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1363..2547)
                     /locus_tag="MJF80_003845"
     CDS             complement(1363..2547)
                     /locus_tag="MJF80_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282444.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1035 a          430 c          385 g          962 t
ORIGIN      
        1 tagcagaagc tcaaactgga acaggaaaaa cactagcttt tttacttccg atatttgaaa
       61 atatatcatt agatataaat gatatacaag tacttatact ttctcctaca agagaactag
      121 ctattcaaat aacagaagaa gctatgaaac ttaaagaatc taaagatgta agtattcttg
      181 cagcatatgg aggaaaagat attggttctc aaataaagaa actaaaagga aatattcata
      241 tgattatagc tacaccaggt aggcttttag accatctaaa tagaaaaaca atagacctta
      301 gtaagttgaa gacttttgtt ttagatgaag cagaccaaat gttgcttatg ggatttaaaa
      361 atgaagtgga agctatttta aaagagactt caaacaaaaa gcaaacacta tgcttttctg
      421 caactataaa ttctcaagta aaaaaattag cttatagata cacaaaaaat cctgttgtag
      481 tttctattca aaaagaagaa atcactctta ataatataaa acaagaagta gtagaaacta
      541 cagatagaaa aaaattagat gccttatgta aggtactaga tgaagataat ccttttatgg
      601 caataatatt ttgtaggact aagagaaggg ttgacaatct tgaagaagct cttgctataa
      661 gaggatataa ttgtcaaaaa cttcatagtg atattgctca atcaaaacga gaaagaataa
      721 tgaaatcatt tagaaattta gatatacaat acttaatagc tacagatgta gcttctcgtg
      781 ggcttgatat atctggagta agtcatatat ataattacga tttaccagaa acaccagaag
      841 actacatcca ccgtattggt agaactggta gagctggtga agaaggatat acatgtgctt
      901 ttattgaccc taaaaatgaa cgaatgttaa gtgaaataga gacggctata gaatctaaaa
      961 ttagtagaag aattatagaa ttataattta tagattgctt aaaataccaa tgccacacaa
     1021 acactgtaat atatagaaag ctacagaaaa catgtatcaa tctatacttt gctgttatac
     1081 tattttatat taaagaaaaa agaggctatc caaagtagga ttagtctctt ttattgtcaa
     1141 aataatgaaa atatatttga cttcatataa aaaagagtgc cttataaact aaaagtccat
     1201 tttgagacac cctcttttta tcttttattc ttagttttat attgcttgtt tatagttgtt
     1261 taatattaga ttttgcaaat aatcttaata aacatgcaga tatattatct atagtatcct
     1321 tatctatagt attctcttta gcaatatttt ttgctactac atctaattta cattttcatt
     1381 taattccgct ggccatttag cttctggatt ttcctttgca aacttcttat tcttataatg
     1441 tcctagtgta aggaatggaa ttactatagt gaatagacca agataaccac aatatccata
     1501 tccatattta acaatattac ttaatcctgt aagtgatata agcattgaca ctaccattat
     1561 tgcacaagct gtaaataccc ttcttttcat tcttgaagaa aggaaagaaa gtgctttatg
     1621 gtcttcaaat ctatttacaa gaccaaatat agtagttact cctgtagaca ttaaacaaag
     1681 gaataatgca acattataaa ttatgtatat agtatgattt ccagattgac ctgcaacata
     1741 aagagtaggg agtgttgttt gcccagcagc aataatttct ctataccatc ccattagcat
     1801 agttacagca agtcctaatg caattgcatt cataacaaat gaaattatca ttgccttagt
     1861 agcttctttt gagttcttaa gaagtggtct tgaacatcct gcaagtgtag gtataacaac
     1921 cgattgaaaa cctgcataaa taaaagtatt aagtattggc ttgatatagc caggtgtgaa
     1981 actcgttcta tttgataata tttcagttat ttctggtact ttagctttta taccaacaat
     2041 aaaaatagta agagtacata ctagaatagc tattgtcatt gctgtagctg ctttagatac
     2101 aagcatagca ccaaatatag taaagacaag aagtacaact ccaataataa atacagctac
     2161 aaaataattt actcctatac tttgaaatac tgctgctgca cctgctataa cagcacttac
     2221 agctattaaa accattagat agtaatatat ctcccatatt atttcaagtt ttggatatgg
     2281 tctccaaagc tcacaaaata aatccttata attcctacaa ttgttattat tatacattat
     2341 gataacttct ctcatggtag cagtaagtat aatcattgct aaaattgcca taagtggtgc
     2401 tgtccatcca tactgcacat agtactgagt ttcttgattc cctgtagcaa atcctcctcc
     2461 tgcatgtgaa ctaaaaagaa cgcttgcaac accaaaaata gctaacatag agcccgttac
     2521 ttttgctttc tttacacttt tttgcatgaa atcatcctcc ctaaaatgat attgaaagtt
     2581 tttattttaa cactttattg ataaaacaat aacatttttg ctcattaaat tcaactatat
     2641 ttttccaatg actttacatt ttcaacttat ttccaccttt atattaaata ttttttatat
     2701 atttatttta ttttttaaat agtattcaaa ttcactttgt tttttaattt tatgaaatat
     2761 cttttttaat gttaaatatt atacattttt tctttattat tatataattt tt
//
LOCUS       DAGSWI010000256         2808 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.261, whole genome shotgun sequence.
ACCESSION   DAGSWI010000256 DAGSWI010000000
VERSION     DAGSWI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.261"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            55..987
                     /locus_tag="MJF80_003846"
     CDS             55..987
                     /locus_tag="MJF80_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBZ0282445.1"
                     /translation="MGFIGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKT
                     KIYSNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSK
                     KAGALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRD
                     KDTSSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNG
                     FTKALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNIT
                     IENAIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            962..1789
                     /gene="panB"
                     /locus_tag="MJF80_003847"
     CDS             962..1789
                     /gene="panB"
                     /locus_tag="MJF80_003847"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBZ0282446.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1808..2656
                     /locus_tag="MJF80_003848"
     CDS             1808..2656
                     /locus_tag="MJF80_003848"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBZ0282447.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1149 a          335 c          448 g          876 t
ORIGIN      
        1 aatgagatta aaaattaaga tgttgtatca caaagtggag gtgatactat taaaataggc
       61 tttattggtt ctggaaaagt tggaacatct ttggcaaatt attttttatc aaaaggttat
      121 tgtgtttcag gcttttatgg aaggaatcaa ttatctttat tagaatcaac aaatttaaca
      181 aaaacaaaga tttacagcaa tttgaaagat ataatttatg aaaacgatat attatttatt
      241 acaacatcag atgattctat tgaaattata gacaaaaaac tttccaaatt taatttaaaa
      301 cataaataca tttgccacac aagtggctct ttaaaatcta gtatattgtt ctgttcaaaa
      361 aaagcaggtg cattaattta ttctatccat cctatatttg cattttccaa taaaaatacc
      421 aacataaaaa aaatgaaaga catttgtttt tccatagaag gtgataatga acaagatgat
      481 ttagttattc aaaatttcat agatgatttg ggaaaccaat tttttataag agataaggat
      541 acttcatcca cttatcattt agctaatgtt ttagtttcaa atttagtatt atctttacta
      601 gatataggag taagttactt tattaaatta ggtttatctg aagaggaatc tttaaaagct
      661 atcagtcctt tagtaaaaaa aaatatagaa aatatacttg ataatggctt tactaaagct
      721 cttactggtc ctgtttctcg tggagatata acaccaataa gaaaacatct atctgtattg
      781 gataaaaaag atgaggatat ttacaaaata ctatccttaa acctactaaa aataattgcc
      841 ttaggaaatg acaattcatt aaaaggtata aaaaatatta ctatagaaaa tgcaatcgaa
      901 aatttaataa gtaaatctga aaaatacaaa gaaatttaca aacttttagg aggaaaaaac
      961 gatgaaaaat accatacaga cttttaagaa tgctaaatat gaaggaaaaa aactatctat
     1021 gcttactgct tatgactact caattgcaaa aataatggat gagtgtgata taaatggtat
     1081 tttaatagga gattcattag gaatggtcat aaaaggtgaa gaaaacactc tatctgttac
     1141 catagacgaa attatatatc atacaaaagc tgtaaaaaat ggtgtaaaaa atgccttaat
     1201 cgtaagtgat atgccttttc tatcttacca tgtttctata gaagatgcag ttaaaaacgc
     1261 tggtagactt ataaaagaag gaggagctca tgctgtaaag ttagaaggtg gctcaaacgt
     1321 tataaagcaa atagaaagca ttgtaaatgc tcaaatacct gtaatggggc atttaggatt
     1381 aactcctcaa tcagtaaatt catttggtgg gtttaaggtt caaggaaata ccagtgaaac
     1441 agcaagacaa cttattgaag atgctaaatt aatagaaaaa gcaggtgcat tctctattgt
     1501 ccttgaagga gtgcctacga aaatagctga gatggtaact aattctattt caattcctac
     1561 aataggtata ggggcaggaa ttaactgtga tggacaaatt ttagtttatc aagatatgtt
     1621 gggaatgttt ggagattttg ttcctaagtt tgtaaagcag tatgcaaata taggagatat
     1681 aatgaaagat tcaattaaaa attacatcct agaagttaat acaggtgcat ttccacaaga
     1741 aaaacacagc ttttcaataa atgaatctga gttagaaaaa ttatactaat attaaggatg
     1801 gtacaaaatg cttgtaaaag aaattaaatt actaagaaat ataatcaaag attggagaaa
     1861 acatggctat tctataggcc ttgttacaac aatgggattt ttacatgaag gacaccaaag
     1921 tcttataaaa aaagctgtaa aagaaaatga taaagtagta gtgagtgtat ttgtaaatcc
     1981 aactcaattt ggaccaaatg aagattttaa tagctatcca agagatatag ataaagattt
     2041 taaatattgt atggatagtg gagcaacagt agtctttaac ccatcaccag aagaaatgta
     2101 tttaaaaggt aattgtacta ccattaatgt ttctggactt acagattttc tttgtggagc
     2161 taaaagacct gttcattttg gaggtgtctg cctagttgtt tcaaagtttc taaatatagt
     2221 aacacctgat aaagcttatt ttggagagaa ggatgcccaa caattagctg taattaaaag
     2281 aatggtcaag gatttaaaca tagatacaga aataataggc tgtcctataa ttcgtgaaaa
     2341 tgatggatta gcaaaaagtt cgagaaatac atatctctct gaggaagaac gaaaatctgc
     2401 tctaatatta aataagagct taagtttagc aaaagaagaa ttagtaaaag gcaatctaaa
     2461 tccagagaat ataaaagaat taataacagc taaaataaat tctgaacact tagcaaaaat
     2521 agactatgtt gaaattgtag atagtgagac attacaacct gttaagcaaa tagaacattc
     2581 tatacttgtg gcaattgctg tgtttatagg aaaaaccaga ctaattgata attttacatt
     2641 tgagttaaat atatagtcta taattttata taatatatct aaaaaataat agaataccta
     2701 aaaagtaata aattacctaa aaagtaataa attacctaaa aaataataaa ttgcttagaa
     2761 aataatagat tacaataact aaaattttaa aattaaattc cttaaaag
//
LOCUS       DAGSWI010000257         2792 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.262, whole genome shotgun sequence.
ACCESSION   DAGSWI010000257 DAGSWI010000000
VERSION     DAGSWI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.262"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(284..1966)
                     /locus_tag="MJF80_003849"
     CDS             complement(284..1966)
                     /locus_tag="MJF80_003849"
                     /inference="COORDINATES: protein motif:HMM:NF022062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HBZ0282448.1"
                     /translation="MANINDFKIVNTYSRKMFEYIGIKKVDSLKESEKTRLGFYHLIL
                     ENITGLRDIDDIKDIIIDTDYNKIVNGEIINDLGIDAVHITDDEIQLFNFKFREKFNE
                     DKTKSENDLSVSMKFLQYIQNNQRLCDLDDKVRFRIEKIRELLDSNYIYNIKLYMVSN
                     EAKGYANNSNVYINTLEENYGMKVVNISLDDIMVFFNVKKENQNCKFMISGDDFLCYE
                     KDKKSSRKSYIVRLSLFDLIRITLSDQELRDNYSIENDYSIDKKKLDYSLLYDNVRGY
                     LGETEYNVKIEQTLKSKHKEFFMFNNGITVTAKNISCDSKNSGMKYLFEIESFQIVNG
                     GQTLRSIYEFFKNEKDDLKFIKLREASILVRIFKIEDDSELKNEIAEYTNSQNSISPA
                     DLKSVDSIQIQIEEYFKANGILYVRKAGMIGDDDYTYENRISKERLAQILYSVNGYPD
                     RASNQKKKLFQEYYDEIFRGENFTLENAIRYSNLYFEIEKIYKDKKEHIEYYDQKTFY
                     IIYMFEKYGCDINESIDILEDALNKHQSSVSNSRKLIQKKFKDYLDSIVEPK"
     gene            2214..2735
                     /locus_tag="MJF80_003850"
     CDS             2214..2735
                     /locus_tag="MJF80_003850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282449.1"
                     /translation="MNILKKWFNLKKEKKEKEYYLKNSNDMDNFKAEYEKIFNYYNSM
                     ENTINVELEQLRLDILIKKQSNPIFSIASTFFAAILSCFFTLALTSFSDYFENILKLS
                     LNLKPTVINVILIILCLIPVLISIYFIIKFIIDISNMGLDMEKKIEFSLIAQQVLNDM
                     KMEQSKKENGNYF"
BASE COUNT          962 a          402 c          251 g         1177 t
ORIGIN      
        1 attttaaggt gtgttgagta atattcttga tagtttatat gttgatgaat ttcaaaaaaa
       61 taagcactct tataaaaaga gtacttttgg tatatattca agcatttatc taatacaatt
      121 atagcatgta ttatgtttta gtatgataat tttcgttcgt tttattattg caacttctac
      181 tatagttttc atattttaac atcaactaaa tgaatctaat taagattact agttaatagc
      241 ctttttgatt tcttcatctt ctagtttaca atctaaaact ttattattta ggttcaacaa
      301 tagagtctag ataatcctta aatttcttct gtattaactt tctagaatta ctaacagaag
      361 attgatgttt gtttaatgca tcctcaagga tatcaataga ttcatttata tcacatccat
      421 atttttcaaa catataaatt atgtaaaatg ttttttgatc ataatattca atatgttctt
      481 ttttgtcttt atatatcttt tcaatttcaa aataaagatt actatatctt attgcatttt
      541 ctaatgtgaa attttctcct ctaaaaattt catcataata ttcttgaaat aactttttct
      601 tttggttaga cgctctatca ggatatccat taaccgaata tagtatttga gctaatcttt
      661 cttttgatat tctattttca tatgtataat catcatctcc aatcatacct gcttttctaa
      721 catataatat tccatttgct ttgaaatatt cttcaatttg aatttgtatg ctatctactg
      781 atttcaaatc ggctggtgaa attgaatttt gactattcgt atactctgct atttcatttt
      841 ttaattcgct atcatcctct attttaaata ttcttactag aatagaagct tctctcaatt
      901 tgataaactt caaatcatct ttttcatttt taaagaactc atatatagac cttaatgttt
      961 gtcccccatt aacaatttga aaactttcta tttcaaatag atatttcatc ccactatttt
     1021 tagaatcaca acttatattt tttgcagtta ctgttatacc attattaaac ataaaaaact
     1081 ctttatgttt gctttttaat gtttgttcta tctttacatt atattcagtt tctcctaaat
     1141 atcctctaac attgtcgtat agcaatgaat agtctaattt ctttttatca attgaataat
     1201 cattttctat agaataatta tctctgagtt cttgatctga tagtgttatc cttattaaat
     1261 caaacaaaga caatcttaca atataagatt ttctactaga tttcttgtct ttttcataac
     1321 acaaaaaatc gtcccctgaa atcataaatt tacaattttg attctccttc tttacattga
     1381 aaaaaaccat aatatcatct aaagaaatgt taactacttt cattccatag ttctcttcta
     1441 gagtattaat atatacatta gaattattag cataaccttt tgcttcatta gataccatat
     1501 acaatttaat attatatata taattactat ccaataattc tcttattttt tctatacgaa
     1561 atcttacttt atcgtctaaa tcacatagcc tctgattatt ttgtatatac tgtagaaact
     1621 tcatgctaac agataaatca ttttcacttt tagtcttatc ttcattaaac ttttctctaa
     1681 atttaaaatt aaataattgt atttcatcat ctgttatatg tactgcatct ataccaagat
     1741 catttattat ttctccattt acaattttgt tataatcagt atcaattatt atatctttta
     1801 tatcatcaat atctcttaat ccagttatgt tttctaatat taaatgataa aatcctagcc
     1861 tggttttttc actttctttt aacgaatcta ctttttttat gcctatatac tcaaacattt
     1921 ttctactgta tgtatttaca attttaaaat catttatatt tgccataaat tctcctctca
     1981 attaactccc actatttaca acaatattaa gtataagaat tttatatact aataagtttt
     2041 ttagatctaa cttcattatt ttgaactaaa tatcaaataa cctataataa aattatatag
     2101 ttatatttta ttgatatcaa ttttctttga tctatttcta caaaaatcgt tcgacaaaat
     2161 aatgaaaaaa atttgattat atgatatata gaatatattg gaggtgacga attatgaata
     2221 tattaaaaaa atggtttaac ttaaagaaag aaaaaaagga aaaagaatac tatttaaaaa
     2281 attctaacga tatggataac ttcaaagcag agtatgaaaa aatttttaat tattataatt
     2341 caatggaaaa taccataaat gttgaattgg aacaattaag acttgatata ttaattaaaa
     2401 agcagtctaa tcctatattc agtatagctt ctactttttt tgctgctatt ttatcatgct
     2461 tttttacttt ggcacttact tcgttttcag attactttga gaatatttta aaattgtctt
     2521 tgaatttgaa gccaacagtt ataaatgtaa tacttataat actttgtctt attccagttc
     2581 taattagtat atattttata attaaattta ttatagacat tagtaatatg ggactagata
     2641 tggagaaaaa aatcgaattc tcacttattg ctcaacaagt tcttaatgat atgaaaatgg
     2701 aacaatcaaa aaaagaaaat ggtaactatt tttagttacc atttcattga acaaacaccc
     2761 ccttcttatt tatctataaa atctaatgct tt
//
LOCUS       DAGSWI010000258         2792 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.263, whole genome shotgun sequence.
ACCESSION   DAGSWI010000258 DAGSWI010000000
VERSION     DAGSWI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.263"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..1068
                     /locus_tag="MJF80_003851"
     CDS             <1..1068
                     /locus_tag="MJF80_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBZ0282450.1"
                     /translation="QRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLLALY
                     LNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWPARF
                     LLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSGELT
                     EKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIGCIK
                     DNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYITEK
                     GYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIEDLV
                     LVTEDGCEILNKHDKEICVVG"
     gene            complement(1171..2013)
                     /locus_tag="MJF80_003852"
     CDS             complement(1171..2013)
                     /locus_tag="MJF80_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBZ0282451.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2197..2769
                     /locus_tag="MJF80_003853"
     CDS             2197..2769
                     /locus_tag="MJF80_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBZ0282452.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
BASE COUNT          994 a          402 c          402 g          994 t
ORIGIN      
        1 caaagattaa atgctgtact tgaacaaatg aaaaaagatg atatttctca aatgttagta
       61 agtgacccta cttctatatt ttatcttaca ggagtactaa tccatccagg cgaaagacta
      121 ttagcattat acttaaattt aaatggcaat aataaattat ttataaatga attattccca
      181 gtttcagaag atttaggtgt tgaaatggtt tggtttaatg atactcaaaa tccagtagaa
      241 ataataactg aacatataga caaaaatgct actatgggtg ttgataaaaa ctggccagct
      301 agattcttat taaacttaat agaattaggt ggaggaagta agtttgtaaa ttcatcttat
      361 ataatagata ctttaagaat gtgtaaagat gaagaagaaa aagagttaat gagaatagct
      421 tcaaaattaa atgacaaggc tatggaacaa cttaaagcta ctgtttctgg tgaacttaca
      481 gaaaaacaac ttgttggtaa attatctaaa atatatgaag acctaggtac tgatggattt
      541 tcatttgacc ctattatagg ttttggacct aatggagcta atccacatgg tgaacctgga
      601 aatgcacttg tgaaaccagg agatgcaata atcttagata taggttgtat aaaagataac
      661 tattgtgctg atatgacaag aactgtattc tataaagaaa tacctgaaaa aggtagagaa
      721 atatttgaaa tagttcttga agctaataag agagctgaag ctattgtaaa accaggtgtt
      781 agattctgtg atatagatgc tgctgctcgt gactacataa ctgaaaaggg atatggacag
      841 tacttcactc atagaactgg tcactctata ggacttgaag ttcatgataa aggagatgta
      901 tcttctataa atacagatac tgtacaacca ggaatgatat tctctataga gccaggtata
      961 tatttaccag gtgagttcgg agttagaata gaagatttag tacttgttac tgaagatggt
     1021 tgtgagatat taaacaaaca tgacaaagaa atatgtgtag ttggttaatt ctaaaatcaa
     1081 tttgataaaa ttaaaaagcc atataaaaat gttttaaaca tttttatatg gctttaattt
     1141 ctcaatctat gatttatttc aatccattat ttatattgca gttattaaga ttgcaccctt
     1201 tgaagtatat gtccccatca ctgaaccaat ttcacagatt acaacatctt tgtattttac
     1261 atccttttcc ataacttccc ttaccttttg agccttttct gggcaattag catgtcctat
     1321 aaataaaacc ttttcttctt tatctttaat ttttgactct actaaagtaa tcagcttttt
     1381 caaactgttt ccttcacctc ttgccttatc tataggagtt acaacgccat ctgcaacttg
     1441 tattatcgcc ttaaaattta aggtatttat aatttttcct gctattggat ttattctacc
     1501 acccttaata gcattttcta aagtttcaag tgttccaaaa aatggaggat tatctttata
     1561 tttatttatt attgaaacaa tctcttcaat ttcttttcct tcttttatta atattgctgc
     1621 ttttaacact aattgtccta ttccaattga cccactaagt gaatctataa cctcaacttt
     1681 cttgttattt ccttcctttt caaacatatc ttttgctaaa agtgcactat tataagtccc
     1741 agataatcct gaacttatat taatcactaa tacactttct tcctgacaat ggtatgcttc
     1801 taaaaattta tcaggagaag gacatgatgt ttttggtagt actttactct cactcatttt
     1861 tctatagaaa gtttcattat ctatttcctt tccagatata taactttctt ctccaaaaga
     1921 tacatttaaa ccaacaactt ctatgttgta tttttctatt atatcatcag gtaagtcaca
     1981 tgcactgtcc actatcaatt ttattttatc catattaacc tcactataag atattatttt
     2041 atttattcag tcggtcgtac gaccgattta attattattt tatatattga atgaaaaaaa
     2101 gtcaatagat atagattctc gctttattga caataaatat ccataagctt ataatgttat
     2161 taatttgtta taaatataca tggaaaggta ggttttatga aaacaaataa tatgaaacag
     2221 gctattttat catctgcaac caatttaata gccaaaaatg gagttcaaaa tactagcctt
     2281 gctgatattg caaaagatgt aaatataagt aaaggtactt tgtattatca ctacgcatct
     2341 aaagatgata ttatatatga tattgcggat actcatttag aagttataac tagtgctata
     2401 ctaaactgtg taaaaaatgt caaatcaaaa aattctcaaa tagaaatggt taacttaatc
     2461 ttagagaaaa tatctactat agaaagtcgt ggtagggtac atatgtatct tatttgtgag
     2521 gcaattactt caaataatga tttaaaggaa agaataagat taaaatatat tgaatggaga
     2581 actactctta aatctgaaat tgttgaatca ctagaaaaat ataatagtag cgaaaatgaa
     2641 caggatgctg aatcattttc ttttttactc atgtcaatag ttgatggttt agttgtccaa
     2701 agtttattaa aaacagaaaa aataccttat gaaaatatag cttctttttt agttaatcat
     2761 tggatttaaa cttaatactt agataattaa ta
//
LOCUS       DAGSWI010000259         2693 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.264, whole genome shotgun sequence.
ACCESSION   DAGSWI010000259 DAGSWI010000000
VERSION     DAGSWI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.264"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..197
                     /locus_tag="MJF80_003854"
     CDS             <1..197
                     /locus_tag="MJF80_003854"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBZ0282453.1"
                     /translation="KIVESNKKKDHTQEANDPVKLYKKQEIKDNLNPKTGDSGTLGFA
                     ALGFSGLLGLVLNKIKFRKK"
     gene            522..1160
                     /locus_tag="MJF80_003855"
     CDS             522..1160
                     /locus_tag="MJF80_003855"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF020596.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HBZ0282454.1"
                     /translation="MSRSKRLIKNNLAKTITTTVMATGVSLSFGLLTVYAEELRTFDK
                     QVIETKSGEIGIVGWRLEDKEGNTIEGYGLGAKKSYELSEKVKPEVATKLTNEIDNSY
                     IYKNEKEENIKTLWVKGIIPPRMGENGDFTKEIVGTKKYYVTSQDKGNGWYDANKSTG
                     NLSIDDFALCGGATASNMLHWWLEQNQDYVYKFIKENPKNGILEFNGEKREI"
     gene            1157..1546
                     /locus_tag="MJF80_003856"
     CDS             1157..1546
                     /locus_tag="MJF80_003856"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF020596.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HBZ0282455.1"
                     /translation="MRDLLNSYDKEKHSSKIFNLFKEYFGRWRRGIWADTTIDMFING
                     YIPSLTGENRGQRNDKNKFSTSKKDHRGGFFRDVFKEEILTERDKINNYEDLSKLVKK
                     RLGEGRVLAISHMMGSNIAHIVTVWGG"
     gene            1530..1841
                     /locus_tag="MJF80_003857"
     CDS             1530..1841
                     /locus_tag="MJF80_003857"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF020596.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HBZ0282456.1"
                     /translation="MYGGADYDSNENLKGLYLSDSDDYNSMFKDKKNNNNKYSMKRYN
                     VENKNGKLRLSTEDKEGFWVEVLELYSLSLGKDTWDKYFKNNNSCKEKVNNIGEIKIL
                     E"
BASE COUNT         1166 a          240 c          431 g          856 t
ORIGIN      
        1 ataagatcgt tgaaagtaat aagaagaaag atcatactca agaagctaat gatccggtta
       61 aattatacaa aaaacaagaa ataaaagaca atttaaaccc taaaacagga gattcaggaa
      121 cattaggatt tgcagcctta ggattttcag ggttacttgg ccttgtttta aacaaaataa
      181 aatttagaaa aaaataaatt atgaatttta aaaagtttat tataaataaa agtaaaaatt
      241 aatactaatt atacttgccc caaattgttt gagaaaaaat tcaacagttg ggggcttttt
      301 acatgccaaa atatgattaa aatcaattgt gatatataaa catataaaaa tgaatttaag
      361 catgagtttg aattgaaaaa ggatcaaact atacttaact tttttacttt tttatataaa
      421 tttcgaaaaa aatataacat tataaaaaca tataataata taaaaaaaat acatatttta
      481 tataaaaaaa gaagtagaaa gggaaaggat gttatataaa catgtcaagg agtaaaagat
      541 tgattaaaaa caatttagca aaaacaataa caacaactgt gatggcaacg ggagtatctc
      601 tctcttttgg tttattaact gtttatgcag aggagctaag aacatttgat aaacaggtca
      661 ttgaaacaaa atcaggagaa ataggtattg taggttggag attagaagat aaagaaggta
      721 atacaataga aggatatggg ttgggagcaa agaaatcata tgaactttct gagaaagtta
      781 aaccagaagt agcaacaaag ttaacgaatg aaatagataa ttcatatatc tataaaaatg
      841 aaaaagaaga gaatataaaa accttatggg taaaaggaat catacctcca aggatgggtg
      901 aaaatggtga ttttactaaa gagatagttg gtacaaaaaa atattatgta acttcacaag
      961 ataaaggtaa tgggtggtat gatgcaaata aatcaacagg taatctgagt atagatgatt
     1021 ttgccctttg tggtggagca acagcctcta acatgcttca ctggtggctt gaacaaaatc
     1081 aagattatgt atataagttt ataaaagaaa atcctaaaaa tggtatattg gaatttaatg
     1141 gagaaaaaag ggagatataa gagacttatt aaattcatat gataaagaaa agcatagtag
     1201 taaaattttt aacttattta aggaatattt tggaagatgg agaaggggaa tatgggctga
     1261 cacgactatt gatatgttta ttaatggtta tataccatca cttacaggtg aaaacagagg
     1321 tcaaagaaat gataaaaaca aatttagcac atctaaaaag gatcatagag gaggattttt
     1381 tagagacgta tttaaggaag aaatactaac agaacgtgat aaaataaata actatgaaga
     1441 tttaagtaag ttagttaaaa agagactagg agaaggacgt gtattagcaa tttctcatat
     1501 gatgggaagt aatatagctc atatagttac tgtatggggg ggctgattat gattctaatg
     1561 aaaatcttaa aggattatat ttatcagatt ctgatgatta taattcaatg tttaaagata
     1621 aaaaaaataa taataataag tattcaatga aaaggtataa tgtagaaaat aagaatggta
     1681 aacttagact atcaacagaa gataaagaag gtttttgggt tgaagtatta gagttatact
     1741 cattatcact aggtaaagat acatgggata aatactttaa aaataataat agctgtaaag
     1801 aaaaagttaa taatatagga gaaattaaaa tactagaata aactcttaaa attagagatt
     1861 gatttttaat aaagattagc ttctagtgta actacatgta tagattttaa taagagctat
     1921 atttatatga taaaacaatt aaataattta gaatatctac tatagaaact gttaaaagac
     1981 actcaaaaaa atttaagttt agataaaagt atgataaata aaatttacat atatatgata
     2041 taaataaagt gggttatatg ccttaattaa tttatccata ttgtatttat ggaatactta
     2101 atttataact ccttatttaa ttagtcccct taagaaaaaa ataatggcca taaagtttaa
     2161 atactttaag gtcaagtggt aaaaagatgg gtagtactac tactcatctt tttattgttt
     2221 tcgtatattt ttattaaata tgtaaaaatg aaaaagttta aaaatcataa atttctattt
     2281 tgaaataagt tgtgaaacaa aaaaatatag ttactccaaa tatttttatg tttttgtgaa
     2341 aaaaaggaat aatattttag tctagatatg ttataactaa agtaggttac ataatgtaac
     2401 taaagatatt ccggctgacg cttaggaata ttggcttagt ttttaattaa ctagctatat
     2461 gttcatttaa ttcacgacct ataagattaa acaataaaaa tgagtattgg aatcaatact
     2521 catttttgct gtttaaataa atagatactt tgtatattta tctaatgata gaattagatg
     2581 aataatttaa gttttaatac tgtatttaat atttttagaa tatattacta tatctatata
     2641 gaattaggtt aaatagaaaa aatctcatta tattattaat ttagaacaaa aat
//
LOCUS       DAGSWI010000260         2627 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.265, whole genome shotgun sequence.
ACCESSION   DAGSWI010000260 DAGSWI010000000
VERSION     DAGSWI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.265"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(695..1324)
                     /locus_tag="MJF80_003858"
     CDS             complement(695..1324)
                     /locus_tag="MJF80_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBZ0282457.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(1403..2365)
                     /locus_tag="MJF80_003859"
     CDS             complement(1403..2365)
                     /locus_tag="MJF80_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBZ0282458.1"
                     /translation="MAKIDNMMSILWILNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
BASE COUNT          878 a          411 c          274 g         1064 t
ORIGIN      
        1 aacattacaa tacagtacta aattagttta tttcatctat catctcaatc atatcattta
       61 ctaactatta ttctaatgag catataacat tgaaatttat aattatacaa cctaaatgcc
      121 tcccaatgta ccctcaaaaa catcatttcc tgtaactgaa gtataattgg tatattttat
      181 cactgaaata aaaaaacact agtcaataaa gactagtatt ttcaagtatt ttatcattct
      241 ttttttaatt attggagacg aatcattaga ataatgccca taatttatct atatgcaaat
      301 tctaaattta aaataaagtt tcagataaat ttactattct atgctatgat acaacagaaa
      361 aaactgaaat ctatgagtgg tagcttcata taatcctagt tcctacagta atcaaaactg
      421 aggtttattc tatatgtaac taatgataaa ttttattata ggagttatta gctctatttg
      481 tgttactata agtagtaatt atatatatga taatttttta acacttttct gttggctata
      541 tagaaagtgg tttagaattt aaatttaaat aaaatttaaa ttcaccaaaa agaaatctat
      601 attagaaacc actactctat tccaccagat tgtaattttt tgttctttat atcacaatgc
      661 tataccatat taaattgaaa aataaatttt aaatttattt gttacaattc attaattcaa
      721 aaaaatattt tgagtcatga gttttgtaaa gatttagcca tgaatttaat gcttctgatt
      781 caaaaacatc taaaagtttc aatacttgaa acgtaaattc caacggagca attcctgaag
      841 ccgtaactaa attttcatca ctaactacag attccgtttt ataatatttt tctccagagt
      901 aatttggtat taccatttta agaaactcta agtcattact tgtatgattt ctaaaatcta
      961 atagcccttt tttagctagg cctaatgtag ctccacatat ggctgcaact atagaatctt
     1021 gtattaaagc ttctttaact ttttctaata ttatctcatg attcatggat aaccaagtat
     1081 ttccccctgg caaaattaat aaatccttac tttccatatt aaactcatca actgtaattt
     1141 caggtaaaac ttttaaccca cccattgtta taataggatt tttatcaatt cctacagtaa
     1201 ttacttttag tggttttaat ccttttttaa aatatcttcc tgtatttaat tcagcagtta
     1261 aatatgccac ttcccaatct gacatagtat caaatacata catatatact tttctatttt
     1321 tcatcttttt tactccttat gtaattaatt tatatatcta attataaaat aacttaactg
     1381 acatattcag tcagttaagc tattaaattt gataataatc aattaattct agtaaatctt
     1441 caattatttt cttcctaagg cttagtggtt caattacttg tattgtcttt ctataaggta
     1501 aaagtatatt tggtatgtac ttgcatatta cttcctcttc aagcataaat attattttat
     1561 taggtgttct ttctttcaag tagtgcccta aaaaccaatg ttgacttaaa ctatctaaag
     1621 aatctatact cccttctaca actaattgaa ttatattttg ttcatctttt atttcaggta
     1681 taattccttt tataaaaaat tcacttgctg aaaattctgt cggtctgtta aatgtatttg
     1741 atgtttctat gatttcacta atcctatcca ctctgaaact acgtacctca tttctcatat
     1801 tacaaaatgc aactacgtac caattattat tccagtgaac aattccatat ggatttataa
     1861 ttctatattt tgattttgct ttacgacctg catggtagtt gatttctaca gatagctcct
     1921 ttaatatact attctccaat tttttaagta ctgattctat agacactttc ccaattggtt
     1981 ttattacttc aagggctctt aagtggtttg ttaaaatatc ttcttgtttt tgatttgaat
     2041 acatttttaa ctttaatatc gatttatcta atgcttcgtc taagaagtat ccttcttttt
     2101 tagcaaatag agctgcatga agtagagatt tttgctcttc tatatcaaaa aaaagaggtg
     2161 ctttagtaaa attatttaaa aggctatacc caccattatg acctgtatct gaaattatag
     2221 gtactccact cgcagaaagt acatcaatat atcggtacac tgttcttata tttatttcta
     2281 atttttcagc tatctgtttt gctgtaattt ttttgtcaga gtttaatatc cacagtatag
     2341 acatcatatt gtcaatttta gccatgtagt ttcacctctt tctatttgtt atataaagtt
     2401 attatataac ttctgatcga ttttttacaa aaaaacatta gtaaactgct aataatttat
     2461 actaagattt atttcaaata tttatagaga gtctttttct atagttaagt tatccattaa
     2521 attccatagt agtgtatcta cttcaattga tatattttca actacacttc tcgttatata
     2581 tcaattttct tttttaaaga ttttacaact gatatttagt tcaagat
//
LOCUS       DAGSWI010000261         2619 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.266, whole genome shotgun sequence.
ACCESSION   DAGSWI010000261 DAGSWI010000000
VERSION     DAGSWI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.266"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            145..429
                     /locus_tag="MJF80_003860"
     CDS             145..429
                     /locus_tag="MJF80_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282459.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(586..759)
                     /locus_tag="MJF80_003861"
     CDS             complement(586..759)
                     /locus_tag="MJF80_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HBZ0282460.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(1200..1415)
                     /locus_tag="MJF80_003862"
     CDS             complement(1200..1415)
                     /locus_tag="MJF80_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0282461.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1426..1893)
                     /locus_tag="MJF80_003863"
     CDS             complement(1426..1893)
                     /locus_tag="MJF80_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBZ0282462.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT         1066 a          253 c          315 g          985 t
ORIGIN      
        1 ttaaaaatta taatttaaag cattaaaaaa taaatttgta ttttgtttta tataaagtaa
       61 tttgaagaat atggaaaaac ttgattagta cattattaat tagggtataa aatggtgtaa
      121 atagaatgat aaggggtgga tgaaatgctt gtatataata aaagttttta tcctaatgac
      181 atatttccaa gattagattt ttcaaaaata aaaaaacagt taaaattgat agataatgac
      241 ctgtcagatt ttggaagcat atgtataata gaaaaagaac attatacaat aagtgtaaac
      301 agtataggtg aaataaatgt gtattatgat ttagagtacg aaaataaggt gtatagaata
      361 gtttatgaga ttgaaaagtt atttaaatct caagttggaa ggtttagcat atctacatac
      421 agaaattgat aattaaaaaa gtagaaatta aaaaacttaa tactaaagat atagataaat
      481 atatgaggta acagctagag agagttgtta ccctttttaa atactaagta tatattattc
      541 ttattaattt tttgtaaatt cactaaaaga cctttttata gaatattatt ttcttttttt
      601 attaaatagg taaatcagta aaaatgcaat tccagttcca attattattc caatgatatt
      661 ttctataatt aattttttta atataaaagt aaataataaa aatgctatac ataaacctaa
      721 aatatatcct ttaaaatagg ttgaaagttt tcttttcata taatcaccaa cttgtatcag
      781 atttagttta cttaattaga gtataacagt gttgtaactt caaagagtat ctaaaagatt
      841 acaaatgtta gataaaatca tcaataatgt aatttataac acgtagctat aaactatgca
      901 ataatataaa tggaggtata agtaaatgaa aaagttccat gttttgctaa cagtttaaat
      961 aaatattagg tagaaaagaa tgtatatagg aatctgtggt aaaattatca atttaaaagt
     1021 attatttagt gtgatacagt tcttataggt aatggataac aaatagataa aggaacagtt
     1081 tctttcttag aacatatttt gttatgactt taaacaaagt taatattaat gtcaataata
     1141 tagtaaaaga ataaaaatca tatagtataa taaaaaagag tgtttctaaa cactcttttt
     1201 taatcatttt catcttctac atattcaagt atatcacctg gctgacagtc taaagctttg
     1261 caaatttcgt ttagagtggt aaatctaata gcttttgctt ttccagtttt taaaattgat
     1321 aaatttgcat ttgtaatatc tattttttct gccaaatctt taagtgatat tttccttttt
     1381 gccatcatta catctaggtt aactactatt gccataacat cacctctata tcgttaaatc
     1441 attatcttct tttattttta tagctttatc aaatgtttct gcaattacaa agcacattag
     1501 tgcaaatata aaagagataa tcataagtgg tgagataccg ccactatcag ttccaaaaat
     1561 tcttatatta ttagtattta aattataacc attgatacat tcaattatag tgtttatcaa
     1621 taaacaaaat cccattacct taaacctttt tacattttca agtacgaatg gggaatactt
     1681 agtactatta attataaata tcaagaataa gatgactata aagtagtcag aaaaatatag
     1741 tattcttgca attaaagcga ttactttgtc agaactaaaa tcatataaaa acataggtat
     1801 catacataag aatattatac cagcaactcc taaaagtgat aatagaaaat atagaaatac
     1861 tgataatata ttactaaaaa attttgtaat cataaaatat catctccttt atataaagta
     1921 tattattgat tataatatac aatttattga atttcaataa taaattattg atataatata
     1981 taaaaccatt gtaatataca ctaaaaataa gaatgtataa ggtggtaaat ttatataaaa
     2041 aagaatatga gttgtaaaat attaaataat attgtactaa ttggaataat tttaacttta
     2101 ttatagatta ttttaatacc attagtactg atggtgtatt ttaaaattgg tataagcatt
     2161 acaaatacga atatgccaac tgtaatatgt aatataagta gctatataca tatgtgctgt
     2221 cccatatatt gtatcattgt ttatattaaa aagattatgt aaattggtat caatcaataa
     2281 atcattttca gaagaaatac ctaaaaactt aaaaaatacc tatatgtgta tttagttaga
     2341 tttgagattt ttacatttaa ttttatagtg acaatattat attattttta tgatgtctat
     2401 ttatatgcaa ttactataat atcagaactt atagtatcat ttgtttcatt atcaataagg
     2461 ttttttactt tagttttgtc aattttatta aaaaaagtta ttgatataaa agataagaag
     2521 gataaaacta tatgacggat ttcaaacata agagtggata tttatataat agatatttta
     2581 tagttaattt tattagattt tatttaatac tgtaatatt
//
LOCUS       DAGSWI010000262         2597 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.267, whole genome shotgun sequence.
ACCESSION   DAGSWI010000262 DAGSWI010000000
VERSION     DAGSWI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.267"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(2..553)
                     /locus_tag="MJF80_003864"
     CDS             complement(2..553)
                     /locus_tag="MJF80_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0282463.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            744..1706
                     /locus_tag="MJF80_003865"
     CDS             744..1706
                     /locus_tag="MJF80_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBZ0282464.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2001..>2597
                     /locus_tag="MJF80_003866"
     CDS             2001..>2597
                     /locus_tag="MJF80_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HBZ0282465.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFS"
BASE COUNT          963 a          280 c          415 g          939 t
ORIGIN      
        1 actaaactgg ataataattt attattactg ctttcaatgt tttttctcct tgattatggt
       61 atgtatgaat tgattcagct gaaaaacaaa tagagtcatt ttctctaagt tgataaatag
      121 agtcatttac ttctaatttt aattgacctt ctataatcat aatatattcc tgtgattttt
      181 cagaatgacc aacagatgta taactatgac cttcttctaa ttccatttga aataattcga
      241 agtttctagt tggtgtattg ggataataac aataaagacg ataatcttta ttttctgaaa
      301 cttgtacatc tatatcagtt tttgatacaa taaaagtttc attttgaggt tgttccagta
      361 ttgcagtata tggaacattt agtccacttg caattttcca aattgtgttt actgttgggt
      421 ttgagtctcc cttttcaatt tgcgaaagca ttactttact tacgccagat aattcagcaa
      481 gttgacctag acttaagtta cgttcattac gtaatctatt taaattttta gcaataacag
      541 tattaatact catagtaact ccttaaaatg taaattattg acaaatataa tttacgaatg
      601 tataatttat ttaacattat ttataattta caaatgtata atataatgaa cgttaaatat
      661 actgtacatc cgtaatataa attatactgc ataaagagtt gtttttcaac ggatgatagt
      721 tgagagatta agggggaatt gtaatgcgtg tacttaataa tgttacagag gctataggca
      781 atactcatat gttacattta tccaaaatag cagatgaatt tggagttcaa ggtaatattt
      841 atgcaaagat ggaatactta aatccgggat ttagtaaaaa ggaccgtgta gcattacaaa
      901 tgattaccga agcagaaaat gcaggtttat tagttccagg tcaagcagtt gtggaattaa
      961 caagtggtaa tactggaaca ggactagcaa tagcttgtgc atgtaaagga tataaattta
     1021 tagcgtgtat gtcaaaagga aattctatgg aaagagcaag aatgatgaaa gctttaggtg
     1081 ctgaagtagt tcttgtagac caaatgccta attcagttca tggacaggta tctggtgaag
     1141 atttggcact tgtagaaatt gaggctcaaa aaatagcaaa agaaagaaat gcatttagag
     1201 cagaccaatt taatttagaa gcttgtaatc atgcacatga aaagtataca gcagaagaaa
     1261 tgtgggaaca attggatgga aatattgatg tatttgtaga ttttcttgga agtggtagta
     1321 cttttgcagg atgttcaaaa acacttaaaa aacataattc ttctatcaaa tgttatgttg
     1381 ttgaacctga aacagcagca atttattcag gtaaagaaat aactgaccaa gggcataaaa
     1441 tacaaggtgg aggatattct atggatttgc cattagtaga caagaaactt attgatggtt
     1501 atattcaagt tacagacgag gaggcaactg atgtagcaag aaaattagct aaattagaag
     1561 gtgtatttgc aggattttct tcaggggcaa atgtatgtgc agcaattaag ttattaaaga
     1621 gcagtgaaaa aggaaaaaat atagttttaa ctcttaatga tagtggttta aaatatttaa
     1681 gtactgattt atatgaagaa ttgtaataat agcacaatag ttatgcaatg ttttttaaat
     1741 taagtatata gatttaaatt ataaaaagta tctcaatgta atttttgaaa gttataaagg
     1801 agatactttt ttattttgta aatatggagt atcctactta taagtattaa gctatataga
     1861 tattgatgat tagctatgca ataaaaaaga gtatgtgttt ttgcatttat acatcataac
     1921 ttggaataat acatattaat gttctaaatg tatttaaaac atatgataaa attagaacaa
     1981 atagatacaa ggagataaaa atgcaaaaga gaattttaag aaattttata tttacgggta
     2041 aaaatatttt aagtaaactt gtttttataa taaaatataa ttttagtaca ttaattttat
     2101 ttgaattatt tcataaagga ttagccttat tttttatatg gccatctatt agatatatat
     2161 ttgatatttt aataaaaagt ttacatatag tctacctaaa tatggataat tttataaaag
     2221 ttttgaccaa tcccatgtca atagttctga taatactatc agttataata ctagcttttt
     2281 atgcattttt tgaatttacc tctgtaatta tttgttttaa taaatcgata aaatatgaaa
     2341 aaatagggtt atttgaactc tttaaaatat catttgagaa ctctataaaa ttgttatatc
     2401 ctaaaaatat actattattt atatttgtga tattaataat tccattagta aatacagttt
     2461 taatttcagg gtttatagga aaaatacaaa taccagagta catactagat tatataaagt
     2521 ctgatataat tttaaatata atctatatga tttttctttt aataatatat atttcggtta
     2581 ttagatggat ttttagc
//
LOCUS       DAGSWI010000263         2577 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.268, whole genome shotgun sequence.
ACCESSION   DAGSWI010000263 DAGSWI010000000
VERSION     DAGSWI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.268"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            109..909
                     /locus_tag="MJF80_003867"
     CDS             109..909
                     /locus_tag="MJF80_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBZ0282466.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            1449..2165
                     /locus_tag="MJF80_003868"
     CDS             1449..2165
                     /locus_tag="MJF80_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282467.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         1007 a          292 c          339 g          939 t
ORIGIN      
        1 aaaaaatttt attccattaa tctactctat atttttcagc atttaagaaa taatttttag
       61 tactttgttt gataatataa ctaagacaac ttttcaggag atgattaaat gacatttaag
      121 aataaaaaat ttactataaa taaatataaa attctatcta ttattctagt tttatgtttg
      181 attttaaccg cttttagtag ctatgtgaat tatagtcata aaaaatttaa agattatgtt
      241 gtcttctatg ttcaacatca agatgatgaa gtgttgtggg ctgggagtgc tattgttaat
      301 gctataaaag aaagaggaaa aaatcatgta tttgtggttt tagtatctac tggatgtggt
      361 atatccgtct ttgataaata taaaaaatat gaaaacctaa caaatataca aaaatcagaa
      421 tatagaaata gagaattttt agcttctctt aatagccttg gcgtaataag ggaaaatata
      481 atattattac cagaaacaaa tacaaatggt aatactgact tcaattacat ggaaaaaatt
      541 gctcttacat ttgaagaaaa atttaaaaat gtaactcata tagcacatac ttataagtta
      601 gatgaccatt tacaacattt aaaaaatggt tctgttatcc aaaatttata taatgctggt
      661 aaaataaaag atgctaaata ttttgtaaaa cctaaatttg ctgataaaat aacttttaat
      721 aataagatag tttataaagc tcattctact gaagactatg aaaaggttaa aaatgcctgt
      781 caacaataca aaattgttga tgaattagaa tatagagaag gtattggcta taagtctgac
      841 cataaatcat ttgataagtt attaaataat aaagatgttc ctgctatact tcatacacct
      901 aatttataag aaatatttat tcttttaatt taagttataa aaaggaaatt atttgagatt
      961 ttgtgatata gcactttttg tgctatacta ctttttctca tacaactatt acccatatct
     1021 aatttcatca tctcttaaaa tatataaaga tatcatcatt aaaacctata agccttatta
     1081 aatgctttat acatacctac attaaactac ctgtttatat taaactacat taaaataaat
     1141 atttgttata aattcttaat aattatgaaa ctcatttgtt atttatattt gttaatatat
     1201 gtttaaacct aaaaaattaa taaaccaaaa taacataaga agtgtgatag attaccctag
     1261 atattttcat aaacttttac cttcagatta atatgggcgt ctatactttt aaatttacta
     1321 tatgcattga caatatatta aaagtactag tttttatttc ataatgaatg ggcttactgt
     1381 tttctaatac ctctttttgg tataaaataa aaattggtta aaaataaaat ttcaaggggg
     1441 ttaataagtt gaatcaaaat atattggttg tagatgatga tagagagata gttgatgcta
     1501 tagagttcta tttgagacct gataacttta atgttttaaa ggcatatgat ggacttgaag
     1561 cattggacat actaatcgaa aatgatatta agttaataat aatggatgta atgatgccca
     1621 atttagatgg gcttaaaact acacttaaaa taagagaaaa aaggaatata ccaataattc
     1681 tcttatctgc aaaatcagaa gatatggaca aaattatagg tcttaatatg ggtgctgatg
     1741 actatataac taaaccattt aatccactag aacttactgc tcgagtaaaa tctcaactta
     1801 gaaggtatat aaatttaggt gattttagta atacaaataa ctcaaatgat actaattata
     1861 ttctaaaaag tggtggccta gagctaaata cagatacaaa aatagtttct ttagatggtg
     1921 aagaaattaa aatgacacca cttgaatata aaatacttac acttcttatg tcacgaaaag
     1981 gtaaaatttt ctcttctcat gagatttatg aaaatgtctg gaatgaggat gcttatagtt
     2041 gtgagagaac tattgctgtt catattagaa gaattagaga aaagattgaa attaatccaa
     2101 aagaaccaaa atatttaaag gtggtgtggg gcattggtta caaaattgaa aaacttaatt
     2161 attaatgatt ctaaagcaaa atttatttta ttattattac tgtttctatt agtttctata
     2221 ttatttgaag cagtttatga tttgaataat aacttttcta atattaatta ttattataga
     2281 gagtttgctc tcccttattt ttattttttc ggtactcctg tacatttgtc aaaagtagaa
     2341 ttagagtttg tctgcatagg tattatatta tcaagttttt tattgactat actatgtttt
     2401 acattgtata aaaaaaataa acaaactaaa gtcaaattct tagagaatgt cataagcctt
     2461 ttttctaagg taaaaataga acttacactt atattaactt tgatactatt aaccttaagg
     2521 tttcatgcat ttacaagtaa taaaatattt tacaacacta atttaataat ctacaat
//
LOCUS       DAGSWI010000264         2555 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.269, whole genome shotgun sequence.
ACCESSION   DAGSWI010000264 DAGSWI010000000
VERSION     DAGSWI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.269"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            45..1643
                     /locus_tag="MJF80_003869"
     CDS             45..1643
                     /locus_tag="MJF80_003869"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBZ0282468.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1878..>2555)
                     /locus_tag="MJF80_003870"
     CDS             complement(1878..>2555)
                     /locus_tag="MJF80_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBZ0282469.1"
                     /translation="TGSIGPMGAIGPTGPTGVTGSIGPMGVTGPTGEIGPTGATGPTG
                     NTGATGSIGPTGVTGPTGATGSIGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAI
                     TGWQTIIGNSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGG
                     KDLAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFR
                     IADTVMT"
BASE COUNT          884 a          382 c          406 g          883 t
ORIGIN      
        1 tttatcaatt attattatat tcaagttttg aaaggagtat taaaatgagt ttattagttt
       61 tcggacataa aaatccagat acagattcaa tatgttctgc aatttcatta gcacatttaa
      121 agaacaaact aggaataaaa gcaacagctt atgctttagg agatatcaga aaagaagcta
      181 aatatgcctt ggattacttt aaggtagatg cacctgaaat tttagataat gttagaatac
      241 aagttagaga tttaaattat gataaagtag tacctttaac accaacttca tctatattag
      301 aggcttataa ccttatggat gaaaaaaatg ttaaaacact acctgtaact tatgatgatg
      361 gaacttttgc tggtatcatt actatgaaag aaatagctaa aaatctactt catcaacact
      421 ttacaactat acacacatca ctatcaaaca tttgtaaaaa ccttaatggt actattcttg
      481 tggactgtgg tgaagatata aaaggtagag tagtcacttt atcatttgga atggagactc
      541 taatagagac tttaaaagaa ggagacatag ctattgttgg agatagatat gatagtattg
      601 aatatgctat tgatacaaaa gttaaacttt taatacttac taatcataca gagatttcaa
      661 aagatttgct agctcttgct aagataaatg gagtgtctgt tgtttctgtt gaaagtgaca
      721 attataaaac ttctaatgta ataaatcaat gtagtttctt agactctata gaagctacag
      781 aaaatttaat tacattcaaa gaagatgatt atatggatga aataaaagag attatgctag
      841 aaacaaactt cagatcttat ccaatattag atgatgataa taagttttta ggtttagttt
      901 caaaaggtca tctattaaac ccatctaaaa agaatgttgt tttagttgac cataacgaat
      961 atgctcaaag tgctgatgga attgaacaag caaacatagt tgaaataatt gaccatcata
     1021 aacttggtgg tatttctact gatgttccta tgtcatttag agttatgcct gttggatgca
     1081 atagtactat aatttatcaa atgtacaaag aaaataatgt tgagatacct tatgaaatag
     1141 ctgggcttct tttatctgct atattatcag atacattatt atttaagtct ccaacaacaa
     1201 ctgatataga taagaaagct tgtgaagaat taagtaaaat agcaaaagta gacatggaaa
     1261 aatacgctat ggatatgttt aaatgcggta cttctttaga tgaatatact atagaagaaa
     1321 tagtaaatat ggactttaaa gaatttaata tgagtggaca tagagttggt attggacaag
     1381 tatttacctt agacatagac tctatatttg ctaaaaaaga tgagttttta tcttatataa
     1441 attcgactga ttatgataaa ttaattcttt cagttacaga tataatcaaa gaaggttctt
     1501 atttaatcta taaagcagaa gatagcttaa ttgctaacgc atttggtgtt gaaggtgttc
     1561 aaggcacatt tgcaccaggt gttgtatcaa gaaaaaaaca attagtccct aatttaacta
     1621 ctgcaatcaa aaattttaaa taatataaaa tttttaaata acgaaaactg tctcaaatct
     1681 atatttgaga cagtttttta ttatatatat attgataata aagttaaata aaaatccata
     1741 aatttaaaat ttttatgaat atattttatt attattgtta ttttattcat tctaaaatag
     1801 gtaaaactca atatataaaa atacttttgt catttaaata aagcctaaat agtttttaag
     1861 attattttag acacgtttta agtcataaca gtatcagcta ttctaaatat cgtcaaagta
     1921 gccgcagttc ctgctgattg cccatttcgt atccctactg ttgtagatga aaaattattt
     1981 attgtaaaag tagtcccacc aaataaaaaa ccagcaaaat atccagaaag tactcctccg
     2041 cctcctggct cagtagtaag ccctgccaaa tcttttcctc ctaaaattgg gaataggata
     2101 gcgaaagaat attgatttat acttgatgaa cctggttgta atgctactga aactgatata
     2161 taatacacac cattttcttg tactgtaaac gtaccatttg catctactgt tatacttgaa
     2221 ctatttccta ttattgtttg ccaacctgtt atagcatctc cattatttag tgtttgtgga
     2281 gatggtgcat ttacgaggaa gttagcaact ggctgagagg aattacctgt tgctcctgtt
     2341 ggtcccgttg gtcctatact tcctgtcgct cctgttggac ctgttactcc cgttggccct
     2401 atacttcctg tcgctcctgt atttcctgtt ggtcctgttg ctcccgttgg tcctatttct
     2461 cctgttgggc ctgttactcc cattggtcct atacttcctg tcactcctgt tgggcctgtt
     2521 ggacctattg ctcccattgg tcctatactt cctgt
//
LOCUS       DAGSWI010000265         2550 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.270, whole genome shotgun sequence.
ACCESSION   DAGSWI010000265 DAGSWI010000000
VERSION     DAGSWI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.270"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(136..2325)
                     /locus_tag="MJF80_003871"
     CDS             complement(136..2325)
                     /locus_tag="MJF80_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBZ0282470.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT          810 a          449 c          239 g         1052 t
ORIGIN      
        1 ttcttataaa taaattttca ccatagtaaa tatatacaga aaattcttgt ttttatgcaa
       61 attgattctt ttataaaaat aagtgtcata aagaaatgtc tgaaatcact ttatgacacc
      121 tatcataata aaaaattatt ctggtaatgc tcttttattt acttttccga ttggtgtaac
      181 tggtatctta tctatcctca ttattattgt tggaatcata tatggaggta gatgctcatt
      241 taaatattct ctaatagttc tttcatttat cttaccacta tcttcctcat caattacata
      301 atatgcacat atatacatat ctccatcatc atttgttctc gatatacaaa caacactttt
      361 aatttctgta atattattta ttatattttc aatctcatat agctctatcc taaactcatc
      421 aatttttact tgattgtcca tccttccaat ataatctaaa tttccatcat ctaaccatct
      481 tactaaatca ccagttctat acattaactt attatgttca caagttttgt aaggattctc
      541 tacaaatact tgctctgtta gccctttttt atttaaatat cctcttgata tttgaatgcc
      601 tgatagacat agctctcctg gtgctccaat tggacatagt ttgttctttt tatctaatac
      661 atatgcataa gtattaaata taggttttcc tattggtatt tttccatatt ctctgtccac
      721 atgaaaagaa gtcgatacaa cagtaaattc ggttggtcca tactcattat atagttcata
      781 attacgcttt ctatatcctt ttaatctttc tcctccggtc tgtacaactc taagtgaatt
      841 attattgcaa ttttgtataa ataaccttgc aaatgctgtt ggcaatgcta gtatagttat
      901 gtcattttcc tcacagtatt catttactgt atattcatca aatttagctt cttcaggcat
      961 aatatctatt gatgcaccta caagcaaata agggaatagt tgtttcatta cagcatcaaa
     1021 actgaaactt aagtatatac cacatatatc attttctgta gcgcttatag attttacgct
     1081 ataataacac atatttacta aactcttatg ttctatagct atggtctttg gcctaccagt
     1141 tgaccctgaa gtagatatcg tatatgcaca atttgatgat gttatttttt cagttaattc
     1201 atatccaagc ttaaacattt cttcatcatt tatatcgagt actatggtat tctcatttac
     1261 aaccttactt ttgagttcat ctgtagttat cactactttt gcattactat cttcaagaag
     1321 gtaattcaat ctttcttctg gattacttgt gtctattgga aaaaatgctc cccctgattt
     1381 cataatccct attgctgtaa taattatgtt aatatctctt ggtaacatta cagctactat
     1441 gtcttcactt tccactccta tatgatttaa ataacctgca agtttatcag ttacttcatc
     1501 caattcttta tatgttaact ttctattttc atccctagca gctatattac taggtgttac
     1561 cttaacttgc tccctaaata aatcaacata acttttatca tagggtattt catattgatt
     1621 gttattaaag tcttccaata atactttttc ttcttcctta catacaaatt ctaaatcttc
     1681 tattaaaata tcattatctt ctattatttg ctctattata taatcaaatt gatttaaaat
     1741 tcttctaata gtttctcttt tatatataga tggtttatac tttattttta ttttaagtgc
     1801 cctcttagta tatactatta cagataaatc atatcctttt ttttctatgt cataatcata
     1861 tgataaatca aaacttttag ctataaatat tgtgtttata agcttagtat ttagatgatt
     1921 tttattctct atatctgata acaaatagta tccatactct aaagacctgc tccactgcct
     1981 ttttagcttc cttacaacat ctataaattt ttcactttgt ctaactctta ctcttattgg
     2041 aattatattc attgttttac caaaaactac atcatcttca tatatatact tttgaagtat
     2101 aaggccatat atagtttcta tgatagagtc tatgctaata ttgttcactt tagaatattg
     2161 aattaacctt tcatgtttct caatattcat cttaaaaacc tcatctacta cttcatcaga
     2221 acaatcttta taaaaaaaat catttggaat ttttgctata ttcgtataat catctaacaa
     2281 atccatccaa taattcatag ctcgttctaa attaacttca tacatatcaa aattccccct
     2341 tactactaag tctatagaaa aaattctttt ggaattttat tttcacaata tataaatata
     2401 tgaataatac atatgcaagc tctaaaaaca tgagcttgca taaaattaca cttattatga
     2461 ctactaaatt cgtaatattt acaatgttta aaatatatta taacacatat atgattttat
     2521 ttttctacat ttatataata tataaattac
//
LOCUS       DAGSWI010000266         2536 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.271, whole genome shotgun sequence.
ACCESSION   DAGSWI010000266 DAGSWI010000000
VERSION     DAGSWI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.271"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            265..1086
                     /locus_tag="MJF80_003872"
     CDS             265..1086
                     /locus_tag="MJF80_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBZ0282471.1"
                     /translation="MKKYIPSVILLCIFEIVAVTLWITMDNLFYMINFTYIGVCISIG
                     LALFANEKKYARRFVQFAVGSYMLIYLGVIQQENMQIEGFWYYLFLGVFEAATIHYTV
                     AKIFGPLIFGRGWCGYACWTVMILDLLPYKQPKSPRKNWGFVRYITFTLSLVLTIGFF
                     LIKIDNLDKIIFLLFIVGNVLYYIVGISFAFVLKDNRAFCKYICPVTVFLKPMSYYSI
                     IRVHCDEDKCIKCGKCLKVCPMNVEVNKDSRKRTNATECILCYECVKECPVNALR"
     gene            1408..>2536
                     /locus_tag="MJF80_003873"
     CDS             1408..>2536
                     /locus_tag="MJF80_003873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282472.1"
                     /translation="MIDINWKKFEVKHPKATDAFESLCYFLFCRKYGLNEGIRTDFNQ
                     VGLETEPIKYTDGKYYGFQSKFFDKNVSYDNIYDSIKKALDNYDNLDYIIIYINKECQ
                     TSCKSAKKIEDMCDSRGIKVEWYLPNNFKLALNQPNNLDLAEFYFGATNVRGLLADSK
                     SIRMTTLLQSKEYLELNLHNYEKTLTTTEYCKEVLSSKKKLHLVSGAAGTGKSVCMQK
                     MLYIYGGFDEETKNKQLEKVKSLGAICIYINLNNTALESLENIVLNYKNTFFADSDNN
                     NFIYLFDGIDEVPTSHITSTVLFIENLLEKENTKKIIISSRLSSYNKYILKASINEIV
                     EYSIENLKEDQVRDYFENKGNQEKVNKLNEVVSNNDNFFEHV"
BASE COUNT          973 a          234 c          425 g          904 t
ORIGIN      
        1 aaaaagatct tcttaagagt aacatgattt caataaaaaa aatcctcaca ttatttttta
       61 aataaataaa aaaatgaatt tgattgcaat atgtaataga cctttacagt tattagatag
      121 tgagatagta tacacacttt aaatacattc tttaaactta taatttaact ttagagagat
      181 gattttcatt gttgtgatat tttgagaatg ttataatgaa aacgtaacaa aacagtaaac
      241 atgattttag aattggggga ttgaatgaag aaatatatac catcagttat tttattatgt
      301 atatttgaaa ttgtggcagt tacattatgg attactatgg ataacttatt ttatatgata
      361 aattttacat atataggagt gtgtatttct atagggcttg cgctgtttgc aaatgaaaaa
      421 aagtatgcaa gaagatttgt tcaatttgct gtgggatctt acatgcttat ttatcttggg
      481 gtaattcaac aagaaaatat gcagatagag ggattttggt attatttatt tcttggagtt
      541 tttgaagcag ctaccataca ttatactgtt gcaaaaatat ttggtccttt aatttttggt
      601 aggggttggt gtggatatgc ctgttggact gtaatgattt tagacttatt accctataaa
      661 cagcctaaga gtccaagaaa aaattgggga tttgttcgat atattacatt tactttatct
      721 ttagttttaa caataggatt ctttttaata aaaattgata atttagataa aataattttc
      781 ttgcttttca tagttggtaa tgtcctatat tacatagtag gaatatcctt tgcctttgtt
      841 ttaaaagata atcgagcttt ttgcaagtat atatgtcctg ttacagtatt tttaaaacca
      901 atgagttatt attctattat ccgtgttcat tgtgatgaag ataaatgtat taagtgtgga
      961 aagtgtttga aagtatgtcc tatgaatgta gaagtgaata aggattcacg aaaaaggaca
     1021 aatgctacgg agtgtatctt atgctatgaa tgtgttaaag aatgtccagt aaacgcatta
     1081 cgttaattat aaaaatccta cagtatctca aattacagaa aaattaaatt tatagaaaat
     1141 gtaatataga atttaaaatt aataatttaa aggattatgt ttaaatctat tataaatata
     1201 atgaagatgt atttagtgga ggtaatgata aagtaatata cttttcaatt cttatatgtt
     1261 tagatctagt gattggcgtc gctgtaaaaa catataatat cagttctata aaagataaat
     1321 attgtagata taaatagaga taaaatttca atgtaattta tgaagttata taattatagg
     1381 aaaaatagat gaagggataa acaaagaatg attgatataa attggaaaaa atttgaggtg
     1441 aaacatccta aagcaactga tgcatttgaa agtttatgct attttttatt ttgtcgtaaa
     1501 tatggattaa atgaggggat tcgtactgat tttaatcagg taggtcttga aacagagccc
     1561 ataaaatata cagatggaaa gtattatgga tttcaatcaa aattttttga caagaatgta
     1621 tcttatgata atatttacga ctctataaaa aaggcgttag ataactatga taatttagac
     1681 tatattatta tatatattaa taaagagtgt caaacttcgt gtaaaagtgc aaagaagata
     1741 gaagatatgt gtgattcaag aggaattaaa gttgaatggt atttacctaa taactttaaa
     1801 ttagcgttga atcaacctaa taatttagac ttagcagaat tttattttgg agcaacaaat
     1861 gttaggggat tacttgctga tagtaagagt atacgtatga ctacattact tcaatctaaa
     1921 gagtatttag aacttaattt acataattat gaaaaaactt taactactac agagtactgt
     1981 aaggaagtat taagttccaa gaaaaaatta catcttgtat ctggagcagc aggtactggg
     2041 aaaagtgtgt gcatgcaaaa aatgttatat atttacggag gatttgatga ggagacaaaa
     2101 aataaacagc ttgaaaaagt aaagtctttg ggagcaatat gtatttacat aaatttgaac
     2161 aatacagcat tagagagttt agagaatata gttttaaatt ataaaaatac tttttttgca
     2221 gatagtgata ataataactt tatatattta tttgacggta tagatgaagt tccaactagt
     2281 catataacat ctacagtatt gtttattgaa aatcttttag agaaagaaaa tacaaaaaaa
     2341 ataatcattt cttcaagatt atcaagttat aataagtata ttttaaaggc ttcaattaat
     2401 gaaattgttg aatattctat tgaaaatttg aaagaggatc aagtaagaga ttattttgaa
     2461 aacaaaggaa atcaagagaa agttaataaa cttaatgaag ttgtttctaa taatgataat
     2521 ttttttgagc atgtta
//
LOCUS       DAGSWI010000267         2520 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.272, whole genome shotgun sequence.
ACCESSION   DAGSWI010000267 DAGSWI010000000
VERSION     DAGSWI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.272"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            246..584
                     /locus_tag="MJF80_003874"
     CDS             246..584
                     /locus_tag="MJF80_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBZ0282473.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(991..2427)
                     /gene="miaB"
                     /locus_tag="MJF80_003875"
     CDS             complement(991..2427)
                     /gene="miaB"
                     /locus_tag="MJF80_003875"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBZ0282474.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT          816 a          386 c          278 g         1040 t
ORIGIN      
        1 aaatttaaca attctaaaat atcaataact atattttgta gcactttttt attttattat
       61 tgtttgaata tttgatggtt ttcaatgcaa tttcaacata aatactatat attagtgtat
      121 actgttgatg aattaaagta tgataatcta tttaataaag tatgaagtaa atgattttaa
      181 tgtgccttta tggtaaaatt actaataagt aataaaatta attaaaatat atggaggtcc
      241 ttataatgtt tgctgtagga tttgttgtta taattgcact tgcctttttc attttaagtg
      301 tactattatt taatggaaaa gccagtatgc tactagcagg ttataactct atgagtaaga
      361 aagaaaaagc acgatacgat aaaaaaaaat tatgtagaag ttctagcatt gttactttta
      421 ttgtctctat aatgctattt attatggcgt ttttgggata tagaatagag agtggtcaaa
      481 tggacgaaaa aaaaatggtt cccttttcta taatatttat aatagtcata ctttccagtg
      541 tattttttaa tatcctctac tctaacaaaa aatgtaaaaa ataaatagtt gtaagttgag
      601 cagttaatta taaaagattg actgcttttt catgtatatt gactactctt attaaatatt
      661 ttaacattta aaaatctatg taaaaataat gaatatagta caaacttcta ataatgattt
      721 tacctttgtg gttacagtcc actatatata atttaatatg ataacaggga attattaatg
      781 acaactaagt aaacttttat aaaatataat aaaacaaaaa tagatgtaca atgaaattat
      841 atcttatatc aaatttattt ttattaatag caaaaactta cattagcata acatatgaca
      901 aattcgctta aaataaaaat ttttaatata atttgttcta tagtacatct attttgatta
      961 gtttacataa ttttattatt ttgtacaata ttaattattt actaatatcc catttaatga
     1021 aaaagttcta ggttcagtaa ttttgaccat aactaattta ccaattaaat catcatttcc
     1081 accttcgaaa tttacgagtt tattttgtct agttctacca gcaaacttat tttcattatt
     1141 tttacttctt ccttcaacta gaacttcaac tattctatct ttatatccat catttatttc
     1201 tgcagaaatc ctatttacag cttctaacac cctattaaat ctatcatgct ttatatcttc
     1261 tggtatttga ttttccattt tagcagcagg agttccctgt cttttagaat aaataaatgt
     1321 aaaagctgaa tcatatctaa ctttttcaac cacatctaaa gtgtccaata aatcttcttc
     1381 tgtttctcct gggaaaccaa tcattaagtc tgtagaaaaa gcaatacctg gaacttcctt
     1441 tttagctttt tctattattt ctaaataata ttcctttgta taatgtctat tcattttttt
     1501 caaaaggcta ctgcttccgc attgtattgg tagatgtaaa aactcacaca ccttatcaca
     1561 atctctaatt gcgtatatta cttcatcaga tatatctttt ggatgagatg tcataaatcg
     1621 aaccctttca attccttcta tatcattcac ttttcttaaa agttctgaga atgaaactgg
     1681 attttcaagt gtcttaccat aagaatctac attttgtcca agtaaagtga tttctttcgt
     1741 tccatttgca acaagctctt ttatctcgtt aattatatct tcaggagttc tacttctttc
     1801 tcttcctctt gtgtatggga ctatacaata tgtgcagaaa ttattgcatc catacattat
     1861 atttacaaat gcttttaatt caaattttct attacttcta agaccttcta ttacttctcc
     1921 atccacatcc catacatcca ctaacatttt gtcagaattt attgattctg taagtaattg
     1981 cggaaattta tatagattat gtgttccaaa tataaggtct acatgtttat atttttttct
     2041 taattcctct acaacatgtg gttgttgcat catacatcca catacagcta ttttcatatc
     2101 tggatttttt ccttttagat gttttaattg tcctaagtta ccatatactt ttagctctgc
     2161 attttctcta actgcacagg tgttatatat tattaaatca ctttcttcaa ccatcattga
     2221 catttggtat cccatctctt ctaacattga acataatttt tctgaatcat gttcattcat
     2281 ttgacaccca aaggtttgag taaacacgag ttttctcttt ccagttctgt tgaagtattc
     2341 ttcattttgt ctgtgtattt gttctatata tttatcttgt tcttgtattt tctctatagg
     2401 tattgttact tgttttcttt tgctcacgtc tattcctcct agttaatttc ttatattaca
     2461 cataacctta attatatcaa ataagaaaaa tcttttaaag atattattat ttttctagtt
//
LOCUS       DAGSWI010000268         2516 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.273, whole genome shotgun sequence.
ACCESSION   DAGSWI010000268 DAGSWI010000000
VERSION     DAGSWI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.273"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            162..1781
                     /locus_tag="MJF80_003876"
     CDS             162..1781
                     /locus_tag="MJF80_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBZ0282475.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTTRQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2155..2349
                     /locus_tag="MJF80_003877"
     CDS             2155..2349
                     /locus_tag="MJF80_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBZ0282476.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT          930 a          294 c          373 g          919 t
ORIGIN      
        1 acaacttaaa aatttaacat tctcttaacg tatacttaac aaaacattaa tatttttatt
       61 gacaaaattt gttattctat attatggaaa caataaattt tagttaataa agtttataat
      121 tcagataaaa aaattattaa tttcttaagg gaggaaatat cgtgaatata gtaattagcc
      181 ttatcggtgg acttggactt tttctttacg gaatgagttt aatgggagaa ggtttacaaa
      241 aatcagcagg agacaaatta aaaaaaatta ttgaactctt aacaagtaat gttgttatgg
      301 gtgtactggt gggtacagtc gttacaggta ttatacaaag ttcaagtgct actactgtta
      361 tggttgtagg atttgtaaat gcaggtataa tgaatctaag tcaagctatt ggtgtaataa
      421 tgggggcaaa tataggtact acagttactg ctcaattagt ttcatttaat cttgaaggaa
      481 ttgcaccaat tgctttaggt ataggtattt tattctattt atttacatca aatcaaaaaa
      541 caaagcattt atctgagata cttataggtt tcggtatatt atttacagga atggagttta
      601 tgaaagatgc agtagctcca cttgctgaat ataaagcatt cactgatgca cttttatatt
      661 ttagtaaaaa tcctgtatta ggtatattgg caggatttgc cataactggt attgttcaaa
      721 gttcaagtgc atctatgggt atgttgatag cactagcttc tcaaggaata cttcctctat
      781 catctgcttt acctattctt tatggagata atataggtac ttgtgttact tcacttttat
      841 caagtgttgg tgcaagtaga aatgctcgac gtgcagctgt aatgcattta agttttaatg
      901 taattggtac aataatattt atgcttgttt taaataaacc tatatcagcc atagttactc
      961 attttgaccc aactgataca actagacaaa tagcaaatgc acatacttta tttaatctta
     1021 caaatgttat aatattatta ccattttcta aatacatagt taaattagca aatagactta
     1081 tacctattaa agaaactgaa tcagaaatag taaataatac taagtactta gatgaaagga
     1141 tgtttagcac tccatctata gcacttggta atactgtaca agaagttgta agaatgggtc
     1201 ataaagctac aaactctttg gaacattcaa ttgcaggatt tttaaataag agcaatgaag
     1261 atattaataa agcttttgag tcagaaaaag ttgtcaataa gttacagaaa gatatattga
     1321 actatttgtt aaaactttct aaagaacctc ttagagatga tgaacgcttt agaacagatt
     1381 tgctttttaa tactgtaaat gacattgaaa gagtctctga ccatgcagaa aatatagctg
     1441 aattggccat gagtgttaag gaaatgaaca ttagtttttc tgatagtgcg ataagagaaa
     1501 tatacgaaat atacaataag acaattacta attttaagga tgccttaata gttttagatg
     1561 ttaaagattt tgagttagca aataaagttt tagaagttga aaatgaagta aattatttag
     1621 aaaaaacttt tagaaattca catatgatta gactaaataa tggaagttgt acaattgatg
     1681 ctggtgtact ttatcttgac ttacttacta atctagaaag aatttctgac cactcaacaa
     1741 atatagtaaa acaagtacta aaacttaaac aaaaaatata gtgattattt ttatatataa
     1801 tcttaatcaa tgagaagtaa gaagctatag ataatgctta tatcatctat ggcttcttat
     1861 tttttatgta atactcaaat ttttatagta ttctcttatt attaaacaat ttctgcctag
     1921 aatattccat atatatatga cttctttcat aaaacttgaa ctgcccataa ctaaagtcac
     1981 aagtatacat aactattttt taatcaatta taaccaatat ttgttttatt tatcttcatt
     2041 ttaattcttc attcaataat gtaataagat tattttttat ttggatacta ttatatcatc
     2101 taaatatgtt attatatatt tggatatttt catattaata ggagggattc tacaatgata
     2161 aattatatgg tagaaattat atatagtgaa aaaataagcg aagtagaaaa catgttgaaa
     2221 attgaaaata tacaatatga atttacttct cgtgatggct ttatattttt tggtttttat
     2281 gatatgacag acgcatatag atttgagact ttatgttcag atgaaaaaat tcgatttaaa
     2341 agaatataat agataaaaaa gtacatattt ctaaactatt tgtttttcaa atttaggttg
     2401 tgttttaatg tagtgttgat attataaaca attttaataa taaaaaagta gggacgttat
     2461 attaatatgc tcctactttt tactcatact tattaatatt atttatcaaa attacc
//
LOCUS       DAGSWI010000269         2501 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.274, whole genome shotgun sequence.
ACCESSION   DAGSWI010000269 DAGSWI010000000
VERSION     DAGSWI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.274"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            346..2130
                     /locus_tag="MJF80_003878"
                     /pseudo
     CDS             346..2130
                     /locus_tag="MJF80_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
BASE COUNT         1088 a          334 c          441 g          638 t
ORIGIN      
        1 tatataatta caagtataaa taataaaatt tatttgtaaa aagagtagca cttttaattc
       61 taaaggctac tttttttata caaaacccta ttttttataa caattcatta acataaaaat
      121 caaacttacc ttaaaatata aatttacaaa tttttcaatc aataagaaaa tttctttatt
      181 ccttacataa tgactgcaat gtaacaaaat aattacaaat taattatgaa atcattatag
      241 ttagaaacct ctttaaaatt agatattaag tatcctttag aattagaacg ggaacaaagt
      301 taaaaaatta agtaatatca aatggacaag ggggaagaaa ataaaatgaa aataccaaag
      361 aagattgctg caatgcttac agttacaatg atagctggaa gttcaacagc aggcatagct
      421 agtgcacaaa ctgtggcaac aaatttaaca gggcaagaaa gatatgaaac tgctgttaaa
      481 ataagtcaag atggatggaa aaatgctgat gaggtagtaa tcgtaaacga ctcatctata
      541 gcagatgctc tatctgcaac accatttgca aaagcaaaaa atgctccaat acttttaaca
      601 agtaaagata aattaaatga taagacgaaa gcagaaatac aaagattaaa agctaaaaaa
      661 gtatacttaa taggtggaac ttctgtatta agtacaaata tagaaaaaga aataaaagat
      721 ttaaaaatat cttttgagag aatctctgga gctgagagat atcaaacatc cttagaatta
      781 gcaaagaaat tagatgctat aagtgatgtt aagaaaatag ctgtagttaa tggtgaaaaa
      841 ggacttgcag atgctgtaag tgtaggagca ccagctgcac aaaacaatat gccagtaata
      901 cttgctgatt ctaaaaatgg aacagcagtt gcagacaaat ttataaaaga tgctggaata
      961 actcaatctt atgtagtagg tggagagagt tcaatttctg aagctgttaa aaataaacta
     1021 ccaaatagta caagactagg tggaacagat agaaatgata caaatgcaaa agtaataaaa
     1081 gaattttata aaaagacaga tttaaaaaat gcttatgtaa ctaaagatgg tatgaataaa
     1141 caagaccaac ttatagatgc actagcagta ggtgtattag gtgctaaaaa tcaatcacca
     1201 gtagttttag ttggaaagaa cttatcagct tcacaaaaat ctctagttaa ctctaagagt
     1261 tttgataaga taactaaagt tggtgggaat ggtaatgaga cagcatttaa tgaaatgaag
     1321 tcattacaag aagtaaaaac agttgaagct aaaacaattt ctgaattaaa atcagctata
     1381 gataaagcta ctgctaatga tgttataaac tttaagccaa ctagtgaggt taaagaagca
     1441 tttacaatac aaactgataa agcagtaaca gtaaacttaa atggtacata tacaaagact
     1501 gtaactatca acatgcctaa tggagatgta aataactatg caaaagtgga tgatgtagtt
     1561 atagatgatg taaaagatgg aacttttgtt aactatggaa aaataactaa cttaaaggtt
     1621 aatgataaaa atggagccaa gatagaaaat aattctaaag gtgagattgg tagtttaaca
     1681 gtagcatctg gagcaagtca agtaaaagtg actaatggtg gtaagataac tacagttaca
     1741 aataattcta aagggactac tatagataat aaaggtacaa taagtagtgt taagggagat
     1801 aattctccaa caataagtgg aaatagtcca agttctaact cttcaggagg aagttcatca
     1861 agtggaggtt cttcacatgg aggaggctca tcttcaggag gaagttcttc taatcaaaca
     1921 tcagtaaata atgaagcagc aaaaataaca agcgtgccta caccagcaaa agatgcaact
     1981 agattaacta tgccatcagt atcaagtgga tatactatag caataaaaac atcaagtaat
     2041 gagagtgtta taaaaaaaga tggaacaata atacctccaa atacagcaac aacagtaaaa
     2101 ttagtattta cggtaacaca tacatcaagt ggtaaaacag cagatacaaa agaattatct
     2161 gtaacagtac cagctaaatc aacagatgaa gaattacaag cagcattaga caatgaagtg
     2221 gcaaaaataa caagcgttcc tgcaccagca aaagatgcaa ctaaattaac tatgccatca
     2281 gtatcaagtg gatatactat agcaataaaa acatcaagca ataagggtgt tataaaggaa
     2341 gatggaacaa taatacctcc aaatacagag gaaacagtaa cattggtatt tacagtaaca
     2401 caagaatcaa gtggtaaaac agcagataca ggagaaatag atgtagtagt accagccaaa
     2461 tcaacagatg gggaaataca agcagaagta caagctgagg c
//
LOCUS       DAGSWI010000270         2459 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.275, whole genome shotgun sequence.
ACCESSION   DAGSWI010000270 DAGSWI010000000
VERSION     DAGSWI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.275"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(277..1476)
                     /locus_tag="MJF80_003879"
     CDS             complement(277..1476)
                     /locus_tag="MJF80_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBZ0282477.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            1595..>2459
                     /locus_tag="MJF80_003880"
     CDS             1595..>2459
                     /locus_tag="MJF80_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBZ0282478.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFL"
BASE COUNT          875 a          374 c          311 g          899 t
ORIGIN      
        1 tcactattag tatagttgct gtaattattc cagtaagaca aattttgaag agtgatatta
       61 tttcagaaat aaataagaat tagagttata taaattttta agattatata actttctgac
      121 aatcttgttt aaattattta aatatatctt atctgtgaac attaaataaa cctcttaata
      181 aatataactc ttgaaaagtc atatctatta agaggtttta ataaattttc atgttaatat
      241 atataatcca aaaaaattaa atcttattaa aacatactat tttgtatagt tatcgaaata
      301 tccttgtatg taaataatag gagtaccctt atctccactt ccagatgtta aatctgctaa
      361 agaacctata aggtctgtta atcttcttgg agtagtacct tgagaaacca tgtttcctgt
      421 taaatcatca gatttattat ctttttcaac tatgtatttt gaaattgctt cttttaactc
      481 atcaccagat aagtcagcaa aatcattatc tgctaagtat tttaatttta cttcgtttgg
      541 agttccctct agcccttttg tataagcagg agatactact gggtcagcta attcccatat
      601 tttacctaca gggtctttaa aagctccatc tccataaacc attacttcta catctttccc
      661 tgttatttct tttatattac cttggatttt gtttacaact tcatcacaat ttatagggaa
      721 taattttact gtttcttctg tagctttgtt tgaacctaaa agtccatact ggtcattgta
      781 accactacca tctacacttg ctgtcattat atcatcaagt gaatatactt ttttagctcc
      841 attttgtctt aaaattctct ttgttctttg tcttgtatgt atatcacaag ttaaaatact
      901 tgttgtatag ttaagaatag tttttggatt gtttgcaaat actatttcta cctctgcacc
      961 acaatccttg attaaattct tataataatc aacataatct acaccagtaa atgtatgctt
     1021 gttatatcca aatagttctc tgtatttttc ttctgttaaa acatctgacc aaggatttat
     1081 tcccttatca tcaagttcat caatacttat taagtgattt ccaacttcat cagaagggta
     1141 gcttaacatt aatacgactt ttctacatcc ttttgcaatt ccttttaaac atatagcaaa
     1201 acgatttcta cttaaaattg ggaatataac cccaacagta tcatctccaa atttatcttt
     1261 tacatctttt gcaatattgt caacgtgagc ataatttccc tgtgctctag ctacaacagc
     1321 ttctgtaacg gcaattacat ctttgtccct aacttcaaag ttttcacttt ttgacgcatt
     1381 taaaacagaa tcaacaacta ttttaactaa gtcatctcct tgtcttataa taggtgctct
     1441 aacccctctt gaaacagttc caactaatct atccattatc taaaatcctc ctaaagaatt
     1501 aatattgtca tattagtacc atgtatatta taattcaaat ataattataa gtaaaataaa
     1561 taatactaat atttgacata aggagtaatt atatatgaat ataaaacttg aactttataa
     1621 ggtttttaat gcagttgtaa ataataagag tttttctctt gctgctaagg aattgtttat
     1681 gtcacagcca gctgtaagcc agtcaataaa gcaattagaa gaacaattag atactttatt
     1741 attttataga aacaataagg gagtaaaatt aactccagag gggaaaattc taagtgaaca
     1801 tgtcactact gcattaaagt taatatcatc aggagaagat agaataaata aatttaaaaa
     1861 attagaatac ggttctctga aaataggagt aggagataca gctgcacgtt ttttcttgtt
     1921 aaaatattta gaaatttttc ataagaagta tccacatata catgtatcta caataaatag
     1981 aacgagtaga gaacttatat ctttactcaa agatgggaat atagatatag caattataaa
     2041 catgccaata gaagatgata cactaaatat tgttgaatgt attgaaatac atgatatatt
     2101 tgtatgtgca aatgattaca tagaatacaa aggtaggaaa atatcattgg aagaattaaa
     2161 cacattgcct ttaataatgc tagaaaataa agcaaattca agactttatg taaatgaata
     2221 ttttttatca aaaggtataa aactaaaccc ggatatagaa ttgggttctc atgaattact
     2281 tttagaattt gcgtatataa atcttggagt gtcttgtgtt atagaagaat ttagcataga
     2341 ttatttggaa aatgagaaat tgtttaaatt agatataaaa gagccaatac ctaagagaaa
     2401 tataggttac tgccatttaa aagatatatc tttatcctta gcaaccaaag aatttttaa
//
LOCUS       DAGSWI010000271         2446 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.276, whole genome shotgun sequence.
ACCESSION   DAGSWI010000271 DAGSWI010000000
VERSION     DAGSWI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.276"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..488)
                     /locus_tag="MJF80_003881"
     CDS             complement(<1..488)
                     /locus_tag="MJF80_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBZ0282479.1"
                     /translation="MKIAIVPGHTLSGKGTGATGYIDEGKENRILTDLIVKWLKQGGA
                     IVYTGKVDKSNNYLAEQCKIANRQNVDLAVQIHFNANKTTLNPMGTETIYKTNNGKVY
                     AERVNDKLATVFENRGAKSDVRGLYWLKHTKAPAILIEVCFVDSKADTDYYIKNKNTV
                     AKL"
     gene            complement(488..745)
                     /locus_tag="MJF80_003882"
     CDS             complement(488..745)
                     /locus_tag="MJF80_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBZ0282480.1"
                     /translation="MDNLISFIPEQLLLLVVALNVLGFGFKKYKQLDNKYIPIILLVI
                     GIGFSIWMLGFNPSSILQGILCWGVAIGANQVYKQLKDGDK"
     gene            complement(765..995)
                     /locus_tag="MJF80_003883"
     CDS             complement(765..995)
                     /locus_tag="MJF80_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBZ0282481.1"
                     /translation="MNEELVKEKISTLETRVKEHGKQIDRIEIEQAKFAIQIQNLCSD
                     IKNLTGVLKWLVGVIITTLVGFFIFAIQKGIF"
     gene            complement(1036..1218)
                     /locus_tag="MJF80_003884"
     CDS             complement(1036..1218)
                     /locus_tag="MJF80_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282482.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNRVFELDDVTNTEYRMAVEN
                     YIIEHSEVVEGAELTK"
     gene            complement(1218..1511)
                     /locus_tag="MJF80_003885"
     CDS             complement(1218..1511)
                     /locus_tag="MJF80_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282483.1"
                     /translation="MERGNRIICDQTGKILLQTGEATGDILEHDEITELHFVDIPYGS
                     IDYTRNRIIGINIETKEPILEEIPTFISDEEKRIQELENQILLNENEKVGGIL"
     gene            complement(1898..2446)
                     /locus_tag="MJF80_003886"
                     /pseudo
     CDS             complement(1898..2446)
                     /locus_tag="MJF80_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899647.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          728 a          436 c          286 g          996 t
ORIGIN      
        1 agttttgcaa ctgtattttt atttttaata taataatctg tatctgcttt actatctacg
       61 aagcacacct caatcaatat cgctggagct tttgtatgct taagccagta aagacctctt
      121 acatctgatt ttgcacctct attttcgaat actgttgcta atttatcatt aactctttcg
      181 gcatatacct taccattgtt agttttgtat attgtctctg tacccatagg atttagagtt
      241 gttttatttg cgttgaaatg gatttgtact gctaagtcta cattttgcct atttgcaatt
      301 ttacattgtt ctgctaagta gttattagat ttatctactt ttccagtata cacaatagct
      361 ccaccttgtt tcaaccattt aacaattaaa tcagttaaaa ttctattttc ttttccttcg
      421 tctatatagc cagttgctcc tgttcctttt ccacttaaag tgtgtcctgg tactattgct
      481 attttcatta tttatcacca tccttcaact gtttgtaaac ttgatttgcc cctattgcaa
      541 ctccccaaca taaaattcct tgtaaaattg atgaaggatt aaatcctaac atccatattg
      601 aaaatccaat tcctatcaca agcaatatga ttggaatgta tttattatct aactgtttgt
      661 atttcttaaa tccaaatcct aatacattaa gagcaactac taataaaagc agttgctctg
      721 gtataaaact tattaaatta tccatatctt aacctcctaa ttaattaaaa tattcctttc
      781 tgtatggcaa atataaagaa ccctactaaa gttgtaatta taactcctac aagccacttg
      841 agtactcctg tcagattttt tatatcacta cataggtttt gtatttgtat agcaaacttt
      901 gcttgctcaa tctcgattct atcaatttgt ttgccgtgtt cttttactct tgtttcaagt
      961 gtacttattt tttctttcac aagttcttca ttcatgaaaa cctcctattg ctttgtatta
     1021 aaaaaagaac attacctatt ttgtaagttc tgctccttct actacctcac tatgttctat
     1081 aatgtagttt tcaactgcca tcctgtactc tgtgttagta acatcatcaa gttcaaatac
     1141 tctatttttt agaggattta atcccttatt taatattctc tctgctaata ttcttactac
     1201 aacattattt atattcatta taaaattcct cctactttct cattttcatt taataaaatt
     1261 tgattttcta actcttgtat tctcttttct tcatctgaaa taaatgttgg tatttcctct
     1321 aaaattggtt cttttgtttc tatatttata cctataattc tatttcttgt atagtctata
     1381 cttccatacg gaatatcaac aaaatgtaat tccgttattt catcatgttc taatatatcc
     1441 cctgttgctt ctccagtttg gagtaatatt ttcccggttt ggtcacatat aattctattt
     1501 cctctttcca tttaaattac cacctttcat attattcata agcataccat gtaaaacttt
     1561 ctccgacttt acaacggtat actacgctac tactatcagt gtattcatct cgataagtat
     1621 ataaagaaaa ttctttgcta gtaatagttt ttacaaacat aaaagcacaa aaacgatatt
     1681 ctccactact cttaggaaca cgaccaacag aagcaagttg atttaaatta ttaatagcat
     1741 aaaaattaac agtataccca tttccataaa aacaaagttt ttcaacaatg acaagactag
     1801 ggatgaaatt taaattaata ggaacagtaa gactcttagc ataattttca gcacttgaat
     1861 accttgaata aaaattttca taatcacttt gagtaaaagt gtaagtaccc ttagcccatc
     1921 ttttacgttg gcttaactga ttatttaaat tagtaatctg attttgaagt tgttcagcag
     1981 tagcatctga actatcaaaa cctgttttta ttttctctga taattcaact aatgtattat
     2041 ttaaatttgc ttctatattt tttaatgcta aagtgtttat aatacttgtt tttccagttt
     2101 taaatcctgc attaacttca attagtttag aagatatgtc ttgcagattc acatcttcgg
     2161 gcaatggcat tatattctta cttatactta acactttttc tgctgtagca ttattactgt
     2221 ctgtaacaac tatcttaagt gtgtgtagtg cattatcttc tagtgtatag ttaattgttt
     2281 tctctgttgt taaatctgtt gttatagttt cttttaacac atcatctata aaatattcta
     2341 tttttgtaag caatgtaggg tctgtgtggt cagctttaaa tgtagctgta atggaattat
     2401 aagaagatac tgttaaaaat ggtaatgctt gtagtaatgt tatttt
//
LOCUS       DAGSWI010000272         2376 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.277, whole genome shotgun sequence.
ACCESSION   DAGSWI010000272 DAGSWI010000000
VERSION     DAGSWI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.277"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            664..1326
                     /locus_tag="MJF80_003887"
     CDS             664..1326
                     /locus_tag="MJF80_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282484.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1335..>2376
                     /locus_tag="MJF80_003888"
     CDS             1335..>2376
                     /locus_tag="MJF80_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBZ0282485.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSI"
BASE COUNT         1051 a          206 c          381 g          738 t
ORIGIN      
        1 ttataaaagt atttttttag atattcaaaa agacattgta agttatatta ataatatggg
       61 tatcaacgaa ttttggatat aatgagtttt tcaacttaaa gttatgtaat aatgaatgta
      121 aagaatattt aatgaaatca ttttgttggt tatgtctagc tgaatacaat aatcttttag
      181 ataaagattt tatatttgaa gataggtgtg aaaaaattaa gaagatatta acatcagcag
      241 tttagaggca aagcatgtaa agtaaagtct gggtaactaa aagtagtaat ttagattgta
      301 actattgtta taaatatgaa aatgaggaaa caggaaatat aagttaggat gtagaaaata
      361 ggttactaaa taaaaagctt aattatataa tcaaagaaat aggtatatac tgtacaattt
      421 catggtgaag aacttttcct aaataaaaag gttggtaaaa gagatataag aaataaaatt
      481 caattataag atatggtatg ataactaaag gcactatatg gagtgaatag gcggaagaat
      541 tttttgaaaa ttataaaaat agtttctgtt gctacatatc aataagtata gatagagatt
      601 gcaaaatcca tagcaaaaat cgaatttata agtgtaaata acatgtaaga caaggagtaa
      661 attatgagta ttttagagat aaaaaaccta aataaaatat atggtaaatc ggaaacagag
      721 gtgaaagcgt taagtcaaat aaatctaaaa ataaatagtg gagaatttgt tgtgatagta
      781 ggtaaaagtg gaagtgggaa aagtacttta ctacacataa tggcaggact tgaaactcca
      841 acaaatggtg atgtgattat tgataatata aatatatcat ctcttgatga aaagaaaaga
      901 tctgcattac gtaaagaaaa gttgggatta atatttcaat catataattt gattccagtt
      961 ttaactgttg aagaaaatat aaaactacca actataaata taaaaaataa agatgaagct
     1021 tatattaatg aattgatgga gttactagga ataaaagata gaagaatgta tttgccaaat
     1081 caattatcag gtgggcaaca acaaagagta gcaatagcaa gggctcttgt aaataaacca
     1141 tcaattgtat ttgctgatga acctactgga aatttagaca caaagacaga gtcagatgta
     1201 ttaagtttat taaaagaatc tcaaaaaaaa tataatcaga caataataat gataacacat
     1261 aatatagata taacaaaata tgctgataga gttatagaaa tagaagatgg atttataaga
     1321 gagtaggtag atttatgaaa cttaattata ttgtaaaaaa atatataatg ggaaataaga
     1381 aaaatacatt attaataatt gtaagtattg taatttcaac agcgttgttt ttaattatga
     1441 atataataag tgaagatgcg acaaacttaa tgataaatca agctaaaaat gaatttacat
     1501 taaaacatgc acaatatatc aatccaaaag acgaagaaat aaaatatata gaaaataaca
     1561 caagtataga taaagttgga aaatcaatgc ttttaggaat tagtgatatt ggtaaaggtc
     1621 aaacactaca aattctatct caggataaag ttgctgaaga actaaatgat atttacacac
     1681 tagaaaaagg taaattacca gtagctgaaa atgaaatcgc tatagattca tggtatatta
     1741 aacaaaaaaa gataaaaaac cctataggaa aaaggataac attagattat agaagacaag
     1801 ggatagatca agaagattta tatactgggg aaaaagaatt taaaataact ggtatcttaa
     1861 atagtaatcc tattttaaaa gctcaaggaa catcaattgg ccttataagt aacgattgtg
     1921 ctatcaagaa tattcctact aaaaataaat atgatcaagt tatgtttact tttaaaaagg
     1981 aaaaaaatat acagagacaa tcacaaaaac ttattaaaaa tggaaatttg aatgagaata
     2041 acatttattt taataatgaa ttattaatag ctatatctga cagtatgaat ttaaaaatac
     2101 cttatataat agttaatata gtactagcac tagcaactat attattaatt tataatattt
     2161 tttatatatt agtatcaaat agaataaagg attttgggat atttagggct ttagggttta
     2221 atccaaatga tatttttaaa ataatgattt tagaagtatc tatatactct attataagta
     2281 tttctatagg tctaatttta ggtgggataa tagctagttt atcaagggag tatgttatag
     2341 gagtaatata caatgtcaac tatataaatt ctataa
//
LOCUS       DAGSWI010000273         2335 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.278, whole genome shotgun sequence.
ACCESSION   DAGSWI010000273 DAGSWI010000000
VERSION     DAGSWI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.278"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            142..1416
                     /locus_tag="MJF80_003889"
     CDS             142..1416
                     /locus_tag="MJF80_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBZ0282486.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAKLQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            1534..2253
                     /locus_tag="MJF80_003890"
     CDS             1534..2253
                     /locus_tag="MJF80_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBZ0282487.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLKHLDYIIASL
                     HRPCIASGTKEKNTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
BASE COUNT          962 a          225 c          442 g          706 t
ORIGIN      
        1 ttttaattta ataagatatt ttctataatg tataaatata tgaagttgaa tttattttaa
       61 aaattatagt aagaaaaatg taagtatttg caatattaat ggtataatat ttacagatat
      121 gataatcaat aagaggaaag gatgtttata atggaaaatg atttaacaat gcaagaatta
      181 cttgaccagc aagaacaagt attcagcaaa gtgaaagttg gagaattgac aactggaaaa
      241 ataactgcag tgagaaatga tgaagtacaa ttaggattag actatgggtt tgatggaata
      301 atacctataa gtgaattgaa catagaaaaa aatcaatata tagaagatat ataccacata
      361 ggtgatgaaa taactgctgt aataacaaaa gtatctcaaa aagatggtac aataacttta
      421 tctaaattac aacttgacaa aagaaatgat tttgccaagt tacaaaaggc ttatgatgaa
      481 catagaataa taactgtaaa tgttgaaaag aatatagata aaggtgtatt tgctaattac
      541 aatacatata cattttttat accaatttct caacttgata caaaatttat tacagataca
      601 tctaaatttg ttggtcttaa cttagaagtt tatataaaag aattggatgt tagaaaaaat
      661 agacttgtag catctcatag agatgtatta caagagcgta taaataaaga gagagaagaa
      721 agaagagctc aaattaaggc agaaaaagaa gctgaaagag caagaattaa gcaagaaaga
      781 gaagaagaaa aagctcgtat caaagcagct aaagaagact tatttaattc tcttgaagtt
      841 ggacaaaaaa gagatggtaa ggtgacaaag ataatgcctt atggtgcatt tgtggatatt
      901 ggtgggattg aaggattagc acatataaat aatttagctt ggacaagagt tgaaagtgtt
      961 gaagatgttg tttcagaggg acaagaagta gaagtttatg tgttggatgt aaataaagag
     1021 actaaaaaaa ttgccttggc attaaaagac ataaataatg acccttggga tttaatagca
     1081 aaagaagtac aaattgatga tgttgtcaat gctaaagttc ttagaataat agaaaaaggg
     1141 gcttttgttc aaattaaaga aggtgtagat gcgtatttac caatatctga attgagtgat
     1201 actagagtgg ctaaagttac taatgttgtt aatattggag atgaggttaa agttaagatt
     1261 cttgatttta aaccaaagac taaaagaatg ttggtttcaa taaaagaagc aactagagag
     1321 ccagaagagg atataacaga atacctagaa gtagaggaat ctttaggaag tattggagaa
     1381 ttatttaaag ataaatttaa agatttagaa gtgtaaaata attgaaaatg gggctgtttt
     1441 aaaacgttac actgtctatt atgacagctt tagttttgaa atagcccttt agctattttc
     1501 acgaatttat gtaaaatatt aggaggaaat ttaatgaaaa agtctattat ggatgtacat
     1561 tgtcacactc taataagtgg acatgcacat agtactttca aggaaaatgt cgaagaggcg
     1621 agtaagaaga atataaaata cttaggaata tcagaccatg gacctaatat gccaggagga
     1681 ccacatccat tctattttta taatttgcat ttgcttccac gtgaagtgca aggcgtaaag
     1741 attcttagag gaatagaagg aaatataatg gattataatg gtaatttaga tgttcaagaa
     1801 gatatgttga aacatttgga ttatataatc gcaagtttgc atagaccttg tatagcctct
     1861 ggtacaaaag aaaaaaatac aaatgctatt ttaaaagtta tggataaacc taaggtaaaa
     1921 ataattggac atccagatga cagtaggtat cctttagatt atgaacctat agtaaaaaaa
     1981 gctaaagata aaaatatcct tttagaaata aataattcat cattaagttc aaactctcat
     2041 agaacgggta cttgggaaaa tgtaagtcat atgttgacat tgtgtaaaac atatggtgca
     2101 agagtaattc ttggtactga ctctcatata tgttattcta taggagaatt tgaaaatgca
     2161 gaaaaagttt tagaagcagt agattttcca gatgaattag tcataaatta tcatgaagat
     2221 gaaattatag aattttttga tataaatttt tagaatcttg ggagtgaaaa aatttgagta
     2281 aaaataagtt tatctatatg aattattatg acaatggtta ttttaaaaaa ggaat
//
LOCUS       DAGSWI010000274         2327 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.279, whole genome shotgun sequence.
ACCESSION   DAGSWI010000274 DAGSWI010000000
VERSION     DAGSWI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.279"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..1191
                     /locus_tag="MJF80_003891"
     CDS             <1..1191
                     /locus_tag="MJF80_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBZ0282488.1"
                     /translation="NLIKGKIIRTIIYSLILSLVVAIIGYAIYYSGIIFIGIWTKYCT
                     DMEFLKNVFINRCIAFEEYFRLILSIFIFILDIGFISATYYQYKGVSISKNNIKKEKK
                     SISKIVFNLVLILLISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAA
                     IEEASHAKADYAEIDVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSF
                     YYYGKGYYDEKFVGEKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKEN
                     NFENQCIVASMDKTILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKT
                     VVEKIHKMNKKIFVWTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVS
                     DYLF"
     gene            1513..2097
                     /locus_tag="MJF80_003892"
     CDS             1513..2097
                     /locus_tag="MJF80_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBZ0282489.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT          906 a          225 c          381 g          815 t
ORIGIN      
        1 aatcttataa agggaaagat tataagaaca attatttatt cattaatatt atctttagta
       61 gtagctatta ttggatatgc gatttattat agtggaatta tatttatagg catatggact
      121 aaatattgta ctgatatgga atttttgaag aatgtattta taaatagatg tattgcattt
      181 gaagaatatt ttagattgat tttgtcaata ttcattttta tattggacat tggatttatc
      241 tcagcaactt attatcaata taaaggagta agtatttcta aaaataatat taaaaaagag
      301 aaaaaatcta tatcaaaaat tgtttttaat ttagtattaa tattattaat ttcttatata
      361 gagtcagttg cattttcttc acatataaat ggtatgttta atacgtcatt ttcattttat
      421 acaacagcaa ttgctcatag agcaggagct gaaatggctc cagagaatac tttggcagca
      481 atagaagaag cttcacatgc taaagctgat tatgcagaaa tagatgttca agaaactaaa
      541 gatggagaac ttattgtact tcatgattca aactttaaga gaactacagg agtagataaa
      601 aatgtttggg atgtaaatta taatgaagta aagacatatg atgcgggaag cttttattat
      661 tatggaaaag gttattatga tgaaaagttt gttggggaaa agataccaac actcaaagaa
      721 atgataaaat attctaaagg taaggtgaaa ttattaatag aaataaaact taatggacat
      781 gaaaagggag acgtagtaaa aaaagttgtt cagttaatta aagaaaataa ttttgaaaat
      841 caatgtatag ttgcatctat ggataaaact atattacaaa aagtaaaaaa actggatgag
      901 aatttactga catgctattt tatggcaatt gcatatggtg atttttcaaa gttaaattat
      961 gttgatatat atggaattga atcaactttt gttaataaaa cagtagtaga aaagattcat
     1021 aaaatgaaca aaaaaatctt cgtttggact gttaatagtg acagtttaat tgagaaaatg
     1081 cttgacctaa atgtagatag tattattaca gataatccat accttataga gagtgcaatt
     1141 tattggaaga aaaatggatt tattagtcaa gtgtcagatt atttatttta aatattctat
     1201 aattgtatta tccagtaaaa gagatattat taaaatcaat aatatctctt tttaacataa
     1261 ataaggttgt taaattaagg ttattcttat aaaaattgtg cttatcagct ataaacaagg
     1321 aataaaagtg aaaaaattga tctgagatta ttacagttaa aagagtttta agatgacaga
     1381 gagatagatt gtcaactgta tatttaataa atcttgttat ttattaaatt ttatataagt
     1441 aaaatatata aattaataaa tcttatgcta gaatatatat gaaaaacaat ataaatttgg
     1501 aggattaaaa acatgacaga gcgagaaaaa atgttggcag gagaacttta cgattgtggt
     1561 gatattgagt tgttaactca atggcataaa gcaaaagacc tagtaagaga ttataaccaa
     1621 acagattcct cggatttgga aaaaaaagaa aaaatattaa atgagttact tggtggtaag
     1681 ggtaaaaact tatggattac tgcaccgtat tatgtagatt atggaaataa tatttatttt
     1741 ggaaataatt gtgaagtcaa tatgaattgt acgtttttag atgataataa aattatcatt
     1801 ggggataatg cactaatcgc accaaacgta caaatatata ctgcatttca tccaactaat
     1861 gctggtgata gatttggtaa tgccaaagaa gatggttcat ttgaattttg taaaactcaa
     1921 acagctccag ttactattgg agataatgtt tggattggcg ggggtgctat tatcatgcca
     1981 ggtgttacta ttggagataa tgtagttata ggtgcaggaa gtattgttac aaaagatatt
     2041 ccaagtaata tgattgcata cggaaatcca tgccgtatta ttcgagaaaa taaataaagt
     2101 taaattttct tgcaaaatct caaaaaacac ctttcttact ttatacatag ggtattttct
     2161 aatactttca catccatatt catttgttaa atttttgtgg aatgtagttt ttatcacttc
     2221 tatcttacat ttccaccatt ttttataaaa gtcattcctt tatagattta tttataatag
     2281 tatatattct atttgatgtt tgaataagca cttattttta agtaaaa
//
LOCUS       DAGSWI010000275         2273 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.280, whole genome shotgun sequence.
ACCESSION   DAGSWI010000275 DAGSWI010000000
VERSION     DAGSWI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.280"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     regulatory      205..315
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            419..790
                     /locus_tag="MJF80_003893"
     CDS             419..790
                     /locus_tag="MJF80_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBZ0282490.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            886..1833
                     /gene="trxB"
                     /locus_tag="MJF80_003894"
     CDS             886..1833
                     /gene="trxB"
                     /locus_tag="MJF80_003894"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBZ0282491.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            1937..2254
                     /locus_tag="MJF80_003895"
     CDS             1937..2254
                     /locus_tag="MJF80_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBZ0282492.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT          952 a          227 c          450 g          644 t
ORIGIN      
        1 aaaaaaaaag aaaatacaag aaatatgcaa tatcaacatt tggaaaaaaa agtaaaaatg
       61 tatacaaaat acatataaaa aaactattgc aaattttata gaatgtttat atacttaagt
      121 caacagagat agaaaaaagc tatttataga aaacaaagga taagttgctt aattttaaat
      181 tttctgaaaa aaataataat ccggatgaag agtatgggag actgctttat aaaaagcgac
      241 cgaagggaca agtttaatct tacttaccca aaagattaga tgaaattctc aggtaaaggt
      301 gactgtactt ggacggggct ctgaaaatga ttttaacagg gaagacaaat tgggagattt
      361 gtttttcctg ttttttattt atcaagtaat aaataaaagt ttgaaagaga gtaaaaaaat
      421 gataataatt acgaataatc caaaagtaaa agaagaagtt caaggcagag aagttttgtt
      481 taaagacaca acttatattg gaatcttaga agcaagtaga gatttgatac atgaaggtta
      541 tgaactttta tctcatccac tctatgggag tgtaaagcca aatgaaacac cttacaggac
      601 tgttatacta aagaaaggaa atcgtttgga tataaactct ttaaccttaa ttgaagaagc
      661 aatcatcact gcaagtaaat ttcagaataa taaaaagact ccaaaatgga cagaaagtgt
      721 tcaagatgat tttagagtaa ttgattatga tatattttat aatacaatac aaagaatgca
      781 gtatgaataa aaaaaattgt taaaaagtta ttaaaacagc ctaaatatta aatctaattt
      841 aatattaaat aaaatgagaa atttaattgg ggaggataag tcaaaatgga aaatgtgtat
      901 gacttagtaa taattggttc aggaccagca gggcttgcag caggtcttta tggagcaaga
      961 gcaaagttaa aaactctaat cctagaaaaa gacaagactg gtggacaaat tgtaataaca
     1021 catgaaattg caaattatcc agggtcagtt ccaaatgcaa caggaccaag tttaatagct
     1081 agaatggttg aacagtgtaa agagtttgga gcagaaatgc taagagacaa cattgttgat
     1141 actgagctag atggagatat aaaagtttta aaaggggaaa aggcagaata tagagcaaaa
     1201 gcagtaataa taggaacagg agcaacacca agaaagattg gttgtcctgg agaaaaagaa
     1261 cttactggta aaggggtttc atattgtgca acttgtgatg cagatttctt tgaagatttt
     1321 gaagtatttg ttgtaggtgg aggagacagt gcattagaag aagcaatgta tttaactaaa
     1381 tttgctagaa aagtaacaat tgtacataga agacaaggat ttagatgtgc taagagtgtt
     1441 gaagaaaaag caaaagctaa tccaaagata gagtttttat tagatacagt aattgaagaa
     1501 atcaaaggtg atggaatatt agaatcagtt gtatttaaaa ataaagttac tggagaaact
     1561 catgaatact ttgcagatga agaagatgga acaatgggag tatttgtgtt cgttggatta
     1621 gatgctcaaa ctgatttatt caaaggtaaa gttgatatgg atgaaaaagg ttacataata
     1681 actgatgaag atatgagaac taacatacca ggcgtatttg cagcaggaga ttgtagatct
     1741 aagactctaa gacaggttgt aactgcaact aatgatggag caattgcatc tatagtagct
     1801 gagaaataca ttgatgaaaa atttgggaac tagaatctgt aaattaagaa atagtgtctg
     1861 taaatttaat gaataatatt taaaattaaa attaatattt aaaattaaaa taaaaatttc
     1921 aagggggaat aagaaaatgt tagatttaga taaggctaca tttgaagaag aagttttaaa
     1981 tgcagaaggt tttgtatttg ttgatttttg gagtgaaggt tgtgagcctt gtaaagcttt
     2041 aatgccagat gttcataaat tagctgagac ttatggggat aaaataaaat tctgtaagat
     2101 ggatacaaca aaagctagaa gattagctat aaaacaaaaa gtattaggtc ttccaactat
     2161 ggctatatat aaagatggag aaaaagtaga cgaagttact aaggatgatg ctacagttcc
     2221 taatatagaa aacatgatta aaaaatatct ttaatatgtt gataaattat taa
//
LOCUS       DAGSWI010000276         2223 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.281, whole genome shotgun sequence.
ACCESSION   DAGSWI010000276 DAGSWI010000000
VERSION     DAGSWI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.281"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(93..1310)
                     /locus_tag="MJF80_003896"
     CDS             complement(93..1310)
                     /locus_tag="MJF80_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBZ0282493.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(1501..1914)
                     /locus_tag="MJF80_003897"
     CDS             complement(1501..1914)
                     /locus_tag="MJF80_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBZ0282494.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT          779 a          340 c          245 g          859 t
ORIGIN      
        1 attttataat agattatatt gtacacttat ataatataaa tataaagtga ttttttagat
       61 tatatatttt aatatacttt cttatataag aattatttta aatcaagatt atctagtatt
      121 tttaaaaata tttttacagc agtatccaag tcatctacag agacactttc attaggatta
      181 tgacttacgc ccccagcgca tcttataaat accataccaa tatcagttat attatccatt
      241 tcctgagcat catggccagc accactataa atataaaaag gattaagatt taaatctata
      301 aaactttttt ctatgatttt tgttatttta ttagaacatg gtacaggaac attttcaaaa
      361 gcaagttcac ttgtataatt aagttttcta ttttcacata tatgtgatat ttcattaaat
      421 atcttttcta cagaattatc aagaatttct tgtgaacaag agcgtacatc gagtgtaaat
      481 tcagctcttt caggtaccac atttgatgaa gatggataga aattcatctt tcctacagta
      541 gcaaccaaat cagtagtcgt tttagctact ttttcgatag ctaatacaca ttcacaggca
      601 caacatccag catcaagtct catattcatt ggaagtgtac cagcatgtcc agacttacca
      661 tttactgata tcttaaaact tttaaatcct gtaatacttg atacaattcc agcaggaaga
      721 gattcatttt ctaaaacagg accctgctca atatgaaatt ctaaatatgc atctacatct
      781 tttctaggat gcaaatttgt taagtttttt gtattaaatc caaactttgt tacagcttct
      841 tcaagtgtaa ttccattttc atcaacagat ttaagaagtt cctctttaaa agtaccagca
      901 aaaactttac ttcccattaa agaagtttca aatctagttc cttcctcttc agcgaaagca
      961 attagcttaa tattgaaagg ataagaccta ttattgtgtt ttgcttcttc aagaccaact
     1021 agaggcacta ttatacctaa cataccatca aatatacctc cacactttac actatcttga
     1081 tgagatgcaa ctactaatgt gggagcattt ggaaagcttg atttatatgt acctataacg
     1141 tttccaatgt catcaatttc aatatcaaga tttaaacctt ccatccatga actaattaaa
     1201 tcaatagatt ttttatgttc ttgagtcaag ataagtcttg taagaccatt tgttgttgaa
     1261 cttatttttc ctaatgtttg tagataatcc atacattttt ttcctaacat atttacctcc
     1321 taaaaatata atttttattt aagctaagat tagcttttat acttataatt tttgaacttt
     1381 caaataattt tgtatgtata gtaaaatact attttagtat taataatgat ataaaaacat
     1441 aaagtctgta gacgaagagc agaagtttta atttaaaaat atgaaatatg atttttatta
     1501 ttatagtatt tttctataat aatatctctt tccagttaga ggataatttt caagtgaaaa
     1561 tactttatgg tatccaaatt tctcataaaa tttaggtgct tgaaaactga atgtatctaa
     1621 aaaaacatat ttacaacctc gttctttagc aattttttca gccttatata aaagttgact
     1681 tccagtacct ttatatctaa gcttctcact aacccataag tattttatag aaagccaatt
     1741 accatgagta acacctataa aacctgcaac aattttacct gctttatctc gaatatatat
     1801 tcctaaatcc actgggtttt tatcttcaag tctagcaaga ttatatttta ataatccttg
     1861 aaaaatagta tcttcatctt gtttttctat tttttctgta atttctaatt ccatatttga
     1921 actttctcct tgtataatct tttgtatatt ggtatttttt aataaaatca gtagaaagaa
     1981 tatagtttaa cattattcta ataggcattt atgctaatgt caaactaata atacttattc
     2041 ttaattttat attgttgata ttaaattttt ttaaataaaa atgtagttta agttatataa
     2101 aaagaaatca tcaataaatt tagtaaacct aaattgttga atattaaaat aagttaattt
     2161 aatacaaaaa aactatttta gatgttattc tattgctatg ttcaataata tcattagtag
     2221 ttc
//
LOCUS       DAGSWI010000277         2197 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.282, whole genome shotgun sequence.
ACCESSION   DAGSWI010000277 DAGSWI010000000
VERSION     DAGSWI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.282"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(36..950)
                     /locus_tag="MJF80_003898"
     CDS             complement(36..950)
                     /locus_tag="MJF80_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBZ0282495.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVDPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            complement(1782..1964)
                     /locus_tag="MJF80_003899"
     CDS             complement(1782..1964)
                     /locus_tag="MJF80_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282496.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT          803 a          274 c          276 g          844 t
ORIGIN      
        1 aaatatgcaa agattatttt aaaagtaatt tttaattaca tgtttttata actctccaat
       61 attctaatat aatgatttgc ctctcttaaa acatggtctg ctaaaagtgg taatattata
      121 gattttatct tacactgttc tattcctgat gctcctgctt ctttaaaatc tttaaactca
      181 acagtttcat ttagagtttc ttctgtaatg ttcttgatat tagaatcagt catttcgttt
      241 gttttttcaa ttaattcatt aaattttata gcaaaatcat ttgaagtatt tattagttca
      301 ccttctgagg ggtctagtaa tcctctcata aacagagcat gttccatcat aatatggtcc
      361 cagaataatt ctatttcttt agcgtttttt gactcaatat ctattttatt ttcaaggtca
      421 actacataag aacgatataa atttgcttca tgtattatat gttcaagaag tagagggtag
      481 tttgatgtaa atatagtaca tgatagtaca ccatctaaga ctctttcttt aaaattaata
      541 agcccatcaa gtaatcttat tgcatcagag ttaagttgag ctacatagtt cactaagtct
      601 tgaccaactt gtgggtctac accactttgt agatttaatt ctcttgtagt gatgtttttg
      661 tttatttcta tccctgtaaa ctcttctgtt ttttgttctg caactgatgt gagagtagtt
      721 acaagttctt ctgaatataa tatatcaggt ctaattatac cattactagc actaacagtg
      781 tatgataata aatcttcaaa ttgcttttta tagtggtcag cttccatagc aagattataa
      841 tttttatttg tgaatcctgc ttctaaaaaa agagcatgct ctttcataat tcttgaaaaa
      901 aataaatgta attctagtga taaaataaca tatttttgat tatctatcat aatttcctcc
      961 ctttagaaag tatcctattt ataatacttt attcgtaata tttaaattta tgtatattat
     1021 aaataaatta actaaattca attgctacta atatcaacag tttttattaa gttatgggcg
     1081 aaaaaattat cttactgtgt ttcttattag atatagatat aatgatttaa ggtacttgtt
     1141 ggctattatt tattaagagt acaagtaata ctagaaattt ttatgagaat tatgtaattt
     1201 ataagtatta ttttgataca aaacaaatgt tttttgtaaa acaagaactt ttatagcaaa
     1261 aaatgaataa atatgaagca agcataattt tataccacaa caattattaa ttctaatagc
     1321 tgcacttaat gttttaggta tgggatttaa aaagtataaa aaattaagtg ataaatatat
     1381 tccattatat tgcttgtgct tggagtagta ttttctgtat agatgttagg atttaatcca
     1441 acttctattt tacaggggat tctatgctgg agtgtttcaa ttggattaaa ttaagtatgc
     1501 aaacaatttc aagacaatga taaataattg gatgtgtgat aggaatatca acaaaaaaca
     1561 ttattaaaaa gaattatttt atttataaaa taaaacaagt aataagaata aataaggagc
     1621 aactaaaatg atttaaatat actagaatta aaaagagaat gtaaagttgt ctagtaatgg
     1681 atgtaaaata ttttaaacga taagctatga aattgatata ttaaaatggg taagaagaat
     1741 attatatagg attcttctta cccattttaa taaatttaat tttatgccaa accacattcg
     1801 catagtagaa tcatcactct atggagacat aactctacaa catcttctcc atatttttta
     1861 ataaaatcac caatttgagt ttctatgaat gaatagtatt cttttaaact agcaatatat
     1921 gcagtcatta tatgaacaca ttcatcaatc ttatctttaa acacaactaa tccccctaaa
     1981 aaacgaaaaa taaaaatatt cagtaaatag cagatattat ttcactcata aatctatata
     2041 gtatgatata ttgcttagat atattttaca agtggatatg taaaactggt tattataatc
     2101 gcaaaactgg ataaactttc attcttaaaa gaaaatttat ccagtttaaa gtttaattat
     2161 gaatatttga gttagtagat atttaatata aattaat
//
LOCUS       DAGSWI010000278         2178 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.283, whole genome shotgun sequence.
ACCESSION   DAGSWI010000278 DAGSWI010000000
VERSION     DAGSWI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.283"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            248..646
                     /locus_tag="MJF80_003900"
     CDS             248..646
                     /locus_tag="MJF80_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282497.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    1082..1345
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1496..2047
                     /locus_tag="MJF80_003901"
     CDS             1496..2047
                     /locus_tag="MJF80_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBZ0282498.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          929 a          197 c          312 g          740 t
ORIGIN      
        1 ctaatttatt tttattataa agtaattata aatggtaaat ttaattgaaa gtagatttaa
       61 cttatacttt atacaccaag tatcataaat taatactact ttgaaaaatc ttaaaaaaag
      121 taaaagttat gataataaaa ataatattaa agataataag atatataaga aacttgaaaa
      181 aatttgtaat ttttagttat atatatatta taatgaacta gttaaaaatt tttatgaggg
      241 ggaaaatatg aataagaaga aaaaattgat tgggatagct atatcttttc tacttataat
      301 cagcattata aatccattac aagtagaagc agaagctatt attgagtcta gtgtatacat
      361 ttcttcaata aaagagaatt atacatatga taatactaaa tttaaaacta ttaaatctaa
      421 aagtagacat aaaagtagga aatataataa aagtaaagta aagttgaaaa aacgaagtat
      481 aagaactaaa aagtatggat atcaaactat aaataaatat aaaagacaaa acgcaagaag
      541 acataatatc ctaacaaaat taatattgtc cataatagtc atatttttgg ttatagtttt
      601 agtagtgcta atatttatta aaagaaagaa gaaaaaattt tactaaaaat aattaaaaga
      661 atatcatttt tttaatcatt ggtatctatt taataatatg atgtaattaa aatggagtaa
      721 tattctgttt caaaggaggc atataaatac aggaatgagt attaaatgat ggttaattaa
      781 gtaaatttat tgataaatgt acttaattaa ctttgaagaa tttaattcct catgaatcaa
      841 aatataaaat tacatcaaca gttattcaca aaattaatat aagaagaatt agagccaaag
      901 agcagactat tagaccatta aagtaattat tcatagaatt ttgtatatga aaagaaatgt
      961 atagtataat ttactttttt tataaaaaat agattacaaa atatattttt tataaaaagg
     1021 tattgacatt ttttagaaaa aagaatatca ttattttaac aacaaaattc aatacaataa
     1081 attaaatcct atgacaagga agagtagtta aatttaggtt cagtatagag agctgaggat
     1141 ggtggaatct tagcaagaaa aaatttgatg aatggccctt agaggaataa attgaaatca
     1201 tagttttgag agtagacttt atcgtcagtc gcacgttata gcgaataagg tatgtaagta
     1261 cctgataatg agatgtatga aaattgaatt ttatattaat ttttatataa tttaggtggc
     1321 aacgcggata atctccgtcc tattagttta ggacggagtt ttttatttta ctgaaaattt
     1381 aaaagataga aggtggtgag aaagatgtta tgaatttcaa aagctaaaga tgatgatata
     1441 gtaggttggc aaaaaaataa attgaaaatt aaaaaaatag gaggaagtac aaaaaatgaa
     1501 aacaaataca aaaaaattaa ctttaaatgc aatactttta gcgatgggat tattaataca
     1561 tcaaatcaca ccagctatag gattaccaat gaagccagat gtaccactag caatgttatt
     1621 tgtaatattg gttttaaata gagatgatta taaaacttgt ttgatagcgg gtatagtaac
     1681 aggaatattt acagcactta catcaagttt tccgggaggt caaattccta atgtaataga
     1741 taaaactctt acagcaaata tagtattttt attaatgaat attagttata atatgccttt
     1801 tataaaaaaa ttagctaaga aaacacaaga tactgtagta gttgcaataa taatgccaat
     1861 aggaactctt gtaagtggaa ctatcttttt attagcagct caagctatag ttggtcttcc
     1921 aggggcttca tttacagctt tattcttagc agtagtatta ccagctgttt taataaatct
     1981 agttgcagga atatttttat tcaagattgt tagtctatca ataagaagag ttagttatca
     2041 agcttaaaca cgaaataata ttttacttta taaagcatta taataactaa ataacataag
     2101 aataaagtag attataacta ttttattctt atgttgttta aagatttggt attatatatt
     2161 tatttttaat aaaatatt
//
LOCUS       DAGSWI010000279         2091 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.284, whole genome shotgun sequence.
ACCESSION   DAGSWI010000279 DAGSWI010000000
VERSION     DAGSWI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.284"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(361..1398)
                     /locus_tag="MJF80_003902"
     CDS             complement(361..1398)
                     /locus_tag="MJF80_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBZ0282499.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSAK"
     gene            complement(1495..>2091)
                     /locus_tag="MJF80_003903"
     CDS             complement(1495..>2091)
                     /locus_tag="MJF80_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBZ0282500.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
BASE COUNT          653 a          326 c          281 g          831 t
ORIGIN      
        1 atgtattgtt tgcaaaaaac gttatcctaa gttataagtg aatgtggaaa ttttatcaaa
       61 atatttacta gatatggaat aactagacat gtatatcaat cgaatatcat aaaaaaaaag
      121 ctgtctggag aatattaaaa ttcttttgga cagctcaagt agtgattaga aagttatata
      181 actttcataa tcagtcgtca atatagacga cttttatgga aaatgaataa ctttatatgc
      241 aatattcaaa ttgctacaat aagcacaatt ttcaaagggt tagggtggat aagagtactt
      301 attatataaa gttttcatat tacctacttc atattaccta ttttttgaaa ttaataagtt
      361 ttattttgca gattttgcat tttcatctga ctttatatac tttatattat ctgctgataa
      421 cttttgcatg tcaagtgctt ttagtgtatc ttcgttaatt attatttcag tttcatctaa
      481 tgtagtaact ggtatatcgc caacagattt tccttctaat acttcaatag caagttctcc
      541 agccttataa cctagttttt cataatctat tccacaacaa gctaaagcac cagaagacac
      601 agaaccttct tcagaggcta ttataggaat cttgttttca ttagcaactt tagaaactat
      661 tggcatagaa gaaactatta aattgtcagt agggacatat aaaacatcta ttttgccaac
      721 taaactagaa atagcttggt taacttcact tgaagagctg attccttttt caactacttc
      781 ataattattt ttcttagcat aatcatgtag agaatcaact tggatttttg aattaacttc
      841 actagtattg tatataaccc ctattttctt tgcttttgga gtcaatgttt taaccaattc
      901 taatgtttta tcaattgaaa gataatcaga tgtaccagaa acatttccac ctggtttttc
      961 aagagattta acaagtccag cttctacagg gtctgtaaca gcagtcatta ttatcggaat
     1021 atctttagta gcattataag ctgcttgtgc agatggagta gatatagcat atatcaaatc
     1081 ttttttgtcg gatacaaact tactagcaat actttgtgta gtaggcatat cattttgtgc
     1141 attttggaaa tctattttta tattatctcc atctttatag cctttatctt caagtgcttt
     1201 gatgaatcct ttctttgctt tatccagaga tggatgttcc actaattgag taatacctat
     1261 atttttttgt tttttgctat ctgtttcttt attttcattt gaagcattgg agccatcatt
     1321 ttgagaacat ccagtaagca ttgatacacc aagtactcct gctataatta aacttaatat
     1381 ttttttactt tttatcattt tattttcctc ccttaaaatt taataattat atttgttcat
     1441 atattcatat gatagattat aaatatttct gattaaaact aagattttaa tttcttatac
     1501 tgcatattca aatttttcta agatttcttc aactgttaaa tctttttttt ctttatcttt
     1561 tacatctaat acaacttcac ccttatgaag cattatcaac ctatctccat attgaagagc
     1621 gtgtttcaag ttgtgagtaa ccataagtgt agttatattt ttctctctta cgattttatc
     1681 tgttaactct attacttcat ttgatgtttt tgggtcaagt gctgctgtat gttcgtctag
     1741 taataagacc ttaggacttg ctaagctaga cataattagt gatagtgact gtctttgacc
     1801 tcctgaaaga tattgtactt taatatctaa atattttttt aaatccaaag aaattccttc
     1861 aagtaagtgt tccaagtcat tcgttttatg acgaagacat ttttttaagt tcaataattt
     1921 ccctttatta agtgctagag ataaattttc tctaaccgtc atagaaggac aagttccaag
     1981 gcttgggtct tgaaaaactc tacttactat ttgagttctt ttatactcag ctaaatttgt
     2041 tatagtagta ctatctaaag taatttgacc agaagattct tttataagcc c
//
LOCUS       DAGSWI010000280         2091 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.285, whole genome shotgun sequence.
ACCESSION   DAGSWI010000280 DAGSWI010000000
VERSION     DAGSWI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.285"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            80..454
                     /locus_tag="MJF80_003904"
     CDS             80..454
                     /locus_tag="MJF80_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBZ0282501.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            451..1314
                     /locus_tag="MJF80_003905"
     CDS             451..1314
                     /locus_tag="MJF80_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282502.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1314..1952
                     /locus_tag="MJF80_003906"
     CDS             1314..1952
                     /locus_tag="MJF80_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBZ0282503.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT          794 a          217 c          309 g          771 t
ORIGIN      
        1 ttctaaaatc agttgacata ttatatatat atttatataa tgtgtatata ctatatatac
       61 aatttaagga ggtgtcttat tgaatatagt tattagtaat tcgagtggga agccaatata
      121 tgaacagatt actacacaaa ttaaaagtat gattataagt ggagagttac cagaaggctc
      181 agctcttcca agtatgagac tattggcaaa agaacttaga ataagtgtaa ttactacaaa
      241 acgagcatat agcgatttag aacgagacgg atttattgag acagttactg gtaaaggcag
      301 ttttgtatct agtaaaaaca tggattttgt caaagaagaa caatttcgtt taattgaaga
      361 atacctacaa aaagcagttg atacagcaaa aagtagtgat attacatatg acgaattaaa
      421 agatatttta tttttattat ataaaggaga atgataagta tggatgcaat aactataaaa
      481 aatttaaata aaacttataa agattttagc ctacaagata tatctttttc agtaccaaaa
      541 gggagtgtaa tgggatttgt aggtgaaaat ggtgcaggaa aaacaactac attaaaagca
      601 attttaaatt taatatctta tgatagtgga aatatagaaa tctttggtct tgatagtaaa
      661 aaaaatgaaa aagaaattaa agagcaaatt ggggttgtat ttgagggaag caactttcat
      721 gaaaatttaa acactgacca tgtttcaaaa atcatgagta aaatctataa aaattggaat
      781 gacaccttat ttaaagacta cttaaaaaaa ttgagagtac cagacaataa attaattaag
      841 gagttttcaa aggggaataa gatgaaactt agtattgctg tagccttatc tcataaacca
      901 aaattattaa tactagatga ggcaactagt tcacttgacc ctatcgttcg tgaagaaata
      961 ctagatatat ttttagattt tatacaagat gaagaacact ctattatctt atcttcacat
     1021 attacaagtg atttagataa gatagcagac tatattacat ttatacataa aggaaaaatt
     1081 gtatttagtg aaaataagga tgaattaatt gatactatgg gagttttaaa atgtaaacct
     1141 agtgattttg ataacctttc aagagaggat tattcatatt atcgtaaaag ccaatttgga
     1201 tatgaagtac ttcttaaaga taaacataga tttatctcaa gacatccaaa ctgtattgtt
     1261 gataatacgt ctatagaaga aattatgtta ttttatgtga gaggtgataa ataatgaaag
     1321 gtctaatttt aaaagattta cttaatttga aaggaaatat aaagtttata cttttattca
     1381 tcattatgtt tggctttatg tcttctttag gagatggaaa tgttaataac tttataggtg
     1441 ttattatagt tttatgtact acaatgattg tttcaacatt ttcatatgat gatttgaata
     1501 aatgggatag ttatgtactt acaatgccta ttaatcgcaa tgatattgta ttaagtaaat
     1561 atttaactat gttaatattt agctttatag gtgttcttgt ttcacttatt gttagtgtaa
     1621 ctattggtta ttttaaaaat acacttatat taaatgaaac actacttatt aatgcactaa
     1681 tcttatcaat ttcagtatgt tttggtagcc ttattctacc acttatttat aaatttggta
     1741 cagaaagagc tcgtttatta atgattttat gctttttagt tccaacacta gctttattag
     1801 tttttaaaag tattttagaa aatattagtt cacctatttc tatagaaatt atacttaata
     1861 cactagtata ttctcttcca tttgttgcta ttttactatt tgtgatttca tattttatat
     1921 catcaaaaat ctatagtaaa aaagaagtat aaaatatact atatatattt ctgtatttaa
     1981 gtatttaaat aaaatactat cgagtttatt ttggtagtat tttatttttt aactattaat
     2041 ctctactagg tatttattat tttattactt atatttaata agactattta c
//
LOCUS       DAGSWI010000281         2088 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.286, whole genome shotgun sequence.
ACCESSION   DAGSWI010000281 DAGSWI010000000
VERSION     DAGSWI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.286"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(62..1354)
                     /gene="brnQ"
                     /locus_tag="MJF80_003907"
     CDS             complement(62..1354)
                     /gene="brnQ"
                     /locus_tag="MJF80_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBZ0282504.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          687 a          357 c          240 g          804 t
ORIGIN      
        1 taaaaaggaa ttgcctatat aatttaaagg caattccata attatcaatt agtttttatt
       61 attatgcagt tttactgctt ttagatggaa taaagtttcc tattattcct gctattacaa
      121 ctggaactat ccagttaaat cctaatgaag caaatggtaa gctattaact agaggaattt
      181 ttacaccaaa acttgttgca actgttaata cacttactaa taatgccatg tatgcagcac
      241 atttgaacac gttgtcattt ttaattttat tactaaataa tgctagtata actagagtta
      301 tagttgctgg atataccata cttagtatag gagaagagaa ttgtattatt gtacttactc
      361 caaagtttga tatgattgca ctaaatacac aaactactat aactatatgc tcatacttta
      421 attttccatc tgtaagtgtt gagaaatatt gaccagctgc tgaagaaaga cctatagctg
      481 ttgttaaaca tgctaatgca actataatag ccaataaaat tttacctgta gttccaagta
      541 aagctgctgt tattgcaact attaaagctg tttgttgaac atcttgtcca tattgttgag
      601 acacagttgc tccaagataa cataatccac cataaactaa tgccattcct attactgcaa
      661 ttacacctgc acttattgtg gctttcatct tttctttctc acctgtataa cctttagcta
      721 ttactgacat gattataatt gatgcaaata cactagctgc caatgcatcc attgtttgat
      781 aaccttgagt tataccttct gcaaatacat tttgtattaa agaactgtct cttatttctc
      841 ctaatggaga tacgatacct ttaactatta atactgccaa agctataagt aaagctggag
      901 ttaatattga cccaactata tctacaactt ttgatggtct tattgttaaa accaatacta
      961 gagcaaagaa tactatagag aatattactg ggcttattcc agaacctatt gtaggtaata
     1021 tacccatttc ataagttgtt gctgctgttc taggtattgc taaaagtggc cctatacaga
     1081 ttaccattgc acatcctaat gcaatactta atcctttgcc tgctcttgag aacatcttcg
     1141 atgtgtctcc attgaactta gttgctgcta taactgctaa taatgccaaa ccaccatctg
     1201 caaaaaggaa tcctatgaaa gctactagcc aactacttcc agataaaact cctagatatg
     1261 gagggaagat taagttccct gcaccaaaaa acattgcaaa tagtgcaaat ccaattacaa
     1321 ttacatcttt aaaattgatt tgtttctcac ccatttaaag cacccccatt tgaattttac
     1381 taccttttac taaaaaataa gtccaatctc ttaattaaat gaaaaccttt tgacgagatt
     1441 ttatttaatt atacattttt gacttaaatt tgtcaattat gtttcataat ttataaaatc
     1501 ttcacaatat ttaatacaac tttcttaatt aaacaaaatc aacctgtata ctttaaacat
     1561 agttggtttt ttgttttttc tctaattttt cttgataaaa ttaactattc cgaataatta
     1621 aaatattaag actttttaga ttattcgatt tattattaag ttgtattgct ttatatactg
     1681 tatatatcac tctccttttt tacattcata taatcttttt aattcaattt aaattttaaa
     1741 tattttgtgt ttattatata atctgaaaaa aaagcagtca atagcatttt taattttttt
     1801 aattttcaaa attatgattc ttaaaaaatc gttttccttt tatcataaca cctatttacg
     1861 ttaattcttg tagtattttg tatttatttt gctaaattga cagttaactt tattcaattt
     1921 ttattctact tacgtagatt tgcctcttgt tttttactct taaaaccatt ttttctgaaa
     1981 attccttatt atgatttatc aacttatttt tcttcataac aattactctc taacttccaa
     2041 cttacttaaa ctttgcagtt gctagtattc acgtttacat acttatat
//
LOCUS       DAGSWI010000282         2013 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.287, whole genome shotgun sequence.
ACCESSION   DAGSWI010000282 DAGSWI010000000
VERSION     DAGSWI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.287"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(49..797)
                     /locus_tag="MJF80_003908"
                     /pseudo
     CDS             complement(49..797)
                     /locus_tag="MJF80_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
     gene            1264..1620
                     /locus_tag="MJF80_003909"
     CDS             1264..1620
                     /locus_tag="MJF80_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0282505.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            complement(1735..1911)
                     /locus_tag="MJF80_003910"
     CDS             complement(1735..1911)
                     /locus_tag="MJF80_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282506.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
BASE COUNT          707 a          278 c          273 g          755 t
ORIGIN      
        1 taaattagta ttaaaaagtt caaaaattta cttagaagga atctataatt agatttctat
       61 gtttgttgag aatgcaatta cagcttttcc aattattttt acttcaactg gcttattatc
      121 taaaataatt acttttacta acatcttgcc tttgcgtttt attgcttctc cttgaactgc
      181 ttcaaatata aactctgtgt tgggtgtagg tattacttta taatgtacaa ggtacgcacc
      241 taagggtcca ttagcatttc ctgtaacagg gtcttctgaa ataccaattg caggagcaaa
      301 cattcttcca tgaacttgta gagtttcttt attatgtaat gtaaaaatat aatatccatt
      361 acattttatt tcttgactta ttttcgataa ttcatataaa tttggagata aactatttaa
      421 tgttgataat tcctttattc caatcataac ttttgaatgt cctgtagatg atatggcaat
      481 aggacaatct gtacgaatat cttcctttaa ataccaaatg cattaagtaa tcttttttga
      541 taggtttgtt gtaatggtgg agatacttca atttttcctt gtgtcataat tatttcataa
      601 tcattatctt tttttataat atcaactggt aatattccag caccagtttt ttgaaatact
      661 gttttgttat tgaattggtt ttctattgct ctaacataat gggcagcaat agtagcatga
      721 ccacatatag gaacctcatt agttggtgta aaaaatctga tatgtacatc ataattttct
      781 tgatgatttt tagccaagtt attgaaaata aaagcagttt ctgaattatt taattctctt
      841 gctatttttt gcatttgtga ttcagataag ccatcagcat ttgtaattac accagctgga
      901 ttacctgtaa atttttcttt tgtaatgagt caacttgata aagacgaaat tttttattca
      961 tattatataa tcctttctaa acattagatt agtttatcta catagaaaac ttgagataaa
     1021 ttattattta ttattatctg tttaaataaa attaataaaa taacatatgt ctatattaaa
     1081 atttaaacat atacatctaa aaaagtagta ttatgtaagg ctctatgcta cactgaacct
     1141 cccatgacta aagtcacgag tgtgcatagc tattttttaa tcaagattat attacgaatt
     1201 agtacgctaa acaagaatgc actttttagt tagatactta ctattttgaa agagaggatt
     1261 gagatggata tagataataa ttttgaagaa tttccaaata ttgaagaaag acaaaaagat
     1321 tttaattgtt caattggttt taccatgact gtgataggaa gtaaatggag ggcaattatt
     1381 ttatggcata ttttaaaaca agagccaata cgttacagtc aattaaaaaa agaagttgtt
     1441 catataagtc ataagatact gtcacaagaa ttaaagtatc ttgaaagaga tggacttatt
     1501 aagaggattt cttatccgac tattcctcca aaagttgaat atttgtctac taaacgtggt
     1561 aaatcattgg aatctatatt attagaatta tgtaattggg gtaaacagta tatgagataa
     1621 acttaactgt ttacttcatg aaaaatatat aatgaagctt attttaatta gttctttaat
     1681 gaattataaa acgtagataa aactaaaaag ggttttctag aaaataaaat ttctttaatt
     1741 atatctgctt accaatacca tcacttgatg caaacaagat tctaccacat tctctccata
     1801 ttctagtata aaatcattta attgtgtatc tataaaattg cagtaatcgc gtgaactgca
     1861 tatatatgca gttagtatat gtgtacatct atccatttta tccataaaca tatatcatat
     1921 atccccctaa aaaacattca taaaaacaaa aagaaaatat ctctttggca actggctgac
     1981 ataactcatt tcataagtct tagtccctat agc
//
LOCUS       DAGSWI010000283         1998 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.288, whole genome shotgun sequence.
ACCESSION   DAGSWI010000283 DAGSWI010000000
VERSION     DAGSWI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.288"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(477..1805)
                     /locus_tag="MJF80_003911"
     CDS             complement(477..1805)
                     /locus_tag="MJF80_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBZ0282507.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          744 a          351 c          213 g          690 t
ORIGIN      
        1 aatattaaac aaataataaa tagcaaaaca actatttgcc acatttttaa tacatacatt
       61 atatattcat actatctaat ttttgttatc aaataaaatt ttctaatctc atttataaat
      121 cactatatta atcaggtaat attttctata taatacaata ttctattata tataacagaa
      181 agatttaatt ttcataaaag ctatgtataa tatttgttgt acagatacca cttagtttga
      241 taaaaaacac tttaaaataa agtattttaa aataaataat tttttagatg tttaatttta
      301 taatcaaaaa actaaaaata aaatgaatac cttaaaataa actttttagt tcatggaacg
      361 ctcttttttt gtaaaatcaa acatatcttt accattttaa caataaaaaa gagacttatc
      421 tctatttgag acagtctctt ttattttaat tatgaagcat ttgtaaacta cttggtttat
      481 ctttttactc ctctagtcat ataagcatac actggtaagc ctatagctgt tattacaaga
      541 cctcctatag atatcatagt agttttcata cctgcaaaac acaattgatt aactacaaca
      601 aatataccac ttgctatagc tattatagga acaataggat ataatggaac tttatactct
      661 cttggtatat ttggatgagt ctttctaagt ttcataaccc ctataaaagt taatacatag
      721 aatatccaag tagcaaatac agctaagtca gttaataaat taaattgacc tgacaaagca
      781 tatatagatg caataacaga tactaatgct attgcatttg ctggaacttg agcagaattt
      841 aatttagaaa atataccata ctttggtaaa gtcttatctg tagctaaagt atatgccacc
      901 cttgaaccag ttagtaaaaa tccatttaat gctccaaaca ctgaaattaa tatacctaca
      961 gatattattt ttccacccat tgaaccaaat ataacttcag caactgctga tgcaggagaa
     1021 gcataatttg ctaattcatt agctggtaat acccaaagat acgctaaatt tattataaaa
     1081 taaacagcca taacaactga caatccacct attatagctt ttggaaggtc ttttccagga
     1141 ttcttcattt ctccagctaa agtaccaaca ttcatccatc catcaaaagc aaataatata
     1201 gcaattaaaa cttgacctag tacactaccc aggcttaatc cttcaccaac cataggtgta
     1261 agtattggat tatttccacc acctttaata aatccaacaa ccattattaa aataagtggt
     1321 attaacttac aaattgtaga aacagtttgt atgactcctc cagtttttga acctaacata
     1381 ttaagccctg caactattac tataattcca attgctattg gaagtaaaag tgatggacta
     1441 ccaattagtg ctgatgcttg ttctccaaat ataacaccta atgctgccat catccctgga
     1501 taaaataata caatttgcat ccatccagtt aaaaaaccta atttttctcc ataaatttct
     1561 tttatatata ccatcattcc accagttttt ggtatcgcaa ctgatacctc tgcagctgta
     1621 agaccagctg ttatagttat gattccagct attaaccatg ccaatatacc aagacctgga
     1681 gctcctccag ttaatgtata gactgcttgt ggtttaaaaa atacgcccgc tcctataacg
     1741 gaaccaacaa cagttgataa tgctgctgat acgccaagag tcttttttag ttcattactt
     1801 cccatttttt tcctcctcat taatcctatt taattttaat ttcatagtta aataagcata
     1861 tattatgcca atatatagaa aaatagcttt attatttatt aaaataaaat aaactctact
     1921 tagctttagt aatcttttat ttttagttat gtttaaatat aaaaaaatta taaaaaccaa
     1981 aaatccaaat ataataat
//
LOCUS       DAGSWI010000284         1969 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.289, whole genome shotgun sequence.
ACCESSION   DAGSWI010000284 DAGSWI010000000
VERSION     DAGSWI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.289"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            159..275
                     /gene="rrf"
                     /locus_tag="MJF80_003912"
     rRNA            159..275
                     /gene="rrf"
                     /locus_tag="MJF80_003912"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            282..356
                     /locus_tag="MJF80_003913"
     tRNA            282..356
                     /locus_tag="MJF80_003913"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:314..316,aa:Asn,seq:gtt)
     gene            361..435
                     /locus_tag="MJF80_003914"
     tRNA            361..435
                     /locus_tag="MJF80_003914"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:394..396,aa:Glu,seq:ttc)
     gene            441..516
                     /locus_tag="MJF80_003915"
     tRNA            441..516
                     /locus_tag="MJF80_003915"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:474..476,aa:Val,seq:tac)
     gene            522..598
                     /locus_tag="MJF80_003916"
     tRNA            522..598
                     /locus_tag="MJF80_003916"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:556..558,aa:Asp,seq:gtc)
     gene            609..683
                     /locus_tag="MJF80_003917"
     tRNA            609..683
                     /locus_tag="MJF80_003917"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:641..643,aa:Thr,seq:tgt)
     gene            698..782
                     /locus_tag="MJF80_003918"
     tRNA            698..782
                     /locus_tag="MJF80_003918"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:732..734,aa:Tyr,seq:gta)
     gene            792..865
                     /locus_tag="MJF80_003919"
     tRNA            792..865
                     /locus_tag="MJF80_003919"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:824..826,aa:Gly,seq:tcc)
     gene            876..952
                     /locus_tag="MJF80_003920"
     tRNA            876..952
                     /locus_tag="MJF80_003920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:910..912,aa:Arg,seq:tct)
     gene            962..1037
                     /locus_tag="MJF80_003921"
     tRNA            962..1037
                     /locus_tag="MJF80_003921"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:995..997,aa:Gln,seq:ttg)
     gene            1049..1124
                     /locus_tag="MJF80_003922"
     tRNA            1049..1124
                     /locus_tag="MJF80_003922"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1082..1084,aa:Lys,seq:ttt)
     gene            1127..1215
                     /locus_tag="MJF80_003923"
     tRNA            1127..1215
                     /locus_tag="MJF80_003923"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1161..1163,aa:Ser,seq:tga)
     gene            1233..1323
                     /locus_tag="MJF80_003924"
     tRNA            1233..1323
                     /locus_tag="MJF80_003924"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1267..1269,aa:Ser,seq:gct)
     gene            1332..1408
                     /locus_tag="MJF80_003925"
     tRNA            1332..1408
                     /locus_tag="MJF80_003925"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1366..1368,aa:Pro,seq:tgg)
     gene            1494..1569
                     /locus_tag="MJF80_003926"
     tRNA            1494..1569
                     /locus_tag="MJF80_003926"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1527..1529,aa:Trp,seq:cca)
     gene            1630..1706
                     /locus_tag="MJF80_003927"
     tRNA            1630..1706
                     /locus_tag="MJF80_003927"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1664..1666,aa:Pro,seq:tgg)
     gene            1713..1789
                     /locus_tag="MJF80_003928"
     tRNA            1713..1789
                     /locus_tag="MJF80_003928"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1747..1749,aa:Ile,seq:gat)
     gene            1793..1868
                     /locus_tag="MJF80_003929"
     tRNA            1793..1868
                     /locus_tag="MJF80_003929"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1826..1828,aa:Phe,seq:gaa)
     gene            1876..1952
                     /locus_tag="MJF80_003930"
     tRNA            1876..1952
                     /locus_tag="MJF80_003930"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1910..1912,aa:Met,seq:cat)
BASE COUNT          500 a          448 c          531 g          490 t
ORIGIN      
        1 tactaatagg tcgaggactt gaccaatatt atttgatgtc attcattaag atatatgtgc
       61 agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt attgacaaaa atagtaaaaa
      121 atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg tggttataat agcggagaag
      181 atacacctgt ttccattccg aacacagaag ttaagttctc tagcgctgat ggtacttggt
      241 gggaaactac ctgggagagt aggacatagc cacgtgaatg ttccaaggta gctcaacggt
      301 ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc cccacccttg gagccattat
      361 ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg cggtaacagg ggttcaattc
      421 ccctacgagt caccatttgt gggatcatag ctcagctggg agagcacctg ccttacaagc
      481 agggggtcac aggttcgagc cctgttggtc ccaccatttg cggcctggta gttcagttgg
      541 ttagaatgcc agcctgtcac gctggaggtc gagggttcga gccccttcca ggtcgccaaa
      601 ataaaaatgc tggtgtggct caacggtaga gcagctgact tgtaatcagc aggttgtagg
      661 ttcgattcct atcaccagct ccatgtaaaa ctaatacgga ggatttcccg agcggccaaa
      721 gggggcagac tgtaaatctg ttgtcatcga cttcggtggt tcgaatccac catcctccac
      781 caattaaata tgcgggtgta gctcaatggt agagttctgg ccttccaagc cagctgtgag
      841 ggttcgatcc ccttcacccg ctccaaagaa agcgtgggtg catagctcag ctggatagag
      901 caacgccctt ctaaggcgtg tgtccggggt tcgaatccct gtgcgctcac cacatatata
      961 ttggggatta gccaagtcgg taaggcacat gactttgact catgtatgcg taggttcgag
     1021 tcctgcatcc ccagccaaat aaaaatatgg tctattagct cagtcggtag agcacctgac
     1081 ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc accaatggag aggtgtccga
     1141 gtggtttaag gagctggtct tgaaaaccag tgacttcgaa aggggccgtg ggttcgaatc
     1201 ccaccctctc cgccaaaaaa atgactgaat ttggagaagt actcaagtgg ctcaagagga
     1261 tcccctgcta agggattagg ctgggtaacc ggtgcgaggg ttcgaatccc tccttctccg
     1321 ccattcaaaa acgaggtgta gcgcagtttg gtagcgcaca tggtttggga ccatggggcc
     1381 gggggttcga gtcccttcac ctcgaccaac ttaagctttt agttcagttg gttagagtgc
     1441 ccaactcatg gctagtaatt ctagggtttg agtccatgaa ggttattctt cataggggtg
     1501 tagctcagtt ggtagagcgt cggtctccaa aaccgtgcgc cgggggttcg agtccctcca
     1561 cccctgccaa ataaaaagga cttgaaaaaa gttggatttt agagtagtat aagatagtaa
     1621 ataagatttc gaggtgtagc gcagtttggt agcgcacatg gtttgggacc atggggccgg
     1681 gggttcgagt cccttcacct cgaccaagaa aagggcctat agctcaggtg gttagagcgc
     1741 acgcctgata agcgtgaggt cgctggttcg agtccagcta ggcccaccat ttgcccagat
     1801 agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga ttccgcctct
     1861 gggcaccagg aatacggcgg tatagcttag ttggctagag cgttcggttc atacccgaaa
     1921 ggtcataggt tcgactccta ttaccgctac catctaacta tatcaataa
//
LOCUS       DAGSWI010000285         1956 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.290, whole genome shotgun sequence.
ACCESSION   DAGSWI010000285 DAGSWI010000000
VERSION     DAGSWI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.290"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(205..1074)
                     /locus_tag="MJF80_003931"
     CDS             complement(205..1074)
                     /locus_tag="MJF80_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBZ0282508.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITSEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
     gene            complement(1379..1849)
                     /locus_tag="MJF80_003932"
     CDS             complement(1379..1849)
                     /locus_tag="MJF80_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBZ0282509.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTVIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIEFVKQCENSKE"
BASE COUNT          678 a          311 c          164 g          803 t
ORIGIN      
        1 tacatcaaaa aataaactaa aaaatatcga aaaataattt aaaagacatc aaaactaaat
       61 cttctaatca gcaattaaaa gtctattttc ttagtttaaa aaataaagat agattttttc
      121 aaaacactgg ataaataggt aaaaaaataa acagttagta aaataaattt ttactaactg
      181 tttattttta ttgtttaata tgtcctataa atcctctaag taatattttc cttgcaaaaa
      241 ttctgattct aaacctagat ttttaaaata atcgaccatg cccatacacg caaatttttc
      301 tgtagaataa tgagtagcac ctataacatt aattaaatta tcttttgcta tttgatgaaa
      361 ctctaatact ggttcaaaat gttttaaagg cttagtaaac ccagtaatat atagatttat
      421 accaagttca gcaacttctc ttgctacaaa tggataactt cctccccctg ctgcaatagc
      481 aactaaacca tcttttaata cagaatctcc ataatcatac aatttcacat catgaccaac
      541 agatttttta attaattcct taatctcttc tattgatttt agtgatgttt tacatataac
      601 tcctacctca attttatcgt catatttaca aaatggtttg actatttcta aacccatagc
      661 ttttgcaaaa ctaacagaag ttgaatagtc actatagttg tctagtggag aatgtaatac
      721 ataaaaagaa attctacgat ttttcatctc ttccatataa gatagtggta tgtcgtgaaa
      781 tggaaaacct tcatctgatg ctatgtatcc cattgcatga tgtgaaaaaa ttaaaatatc
      841 tttttcatct ttatctataa cctgcttaat tatttcttta tcaggaaaag tagttgtgta
      901 tactttatta atattttgag cattatctaa tactaatcct atgtattttt ttctaaattc
      961 tgaagtgata aaatcatttt caaaattcat aaaactccaa tcatcattaa tatttttaat
     1021 attaaattct aaatctaatt ttttataaag ttcaattgcc ttaataataa ccactccttt
     1081 ataataatat tgaattacta catattaata tagaaatttc acgataataa actttatacg
     1141 tttaagtatt cctacttaaa attaaaaata ttataaatat tcaaatttat taatatattt
     1201 taatattata tttatttttt ctttattaat attcccctta tttttattta ttttttcttt
     1261 attaatattt tctttatttt aatttaattt atgacatttg tcaaatacta gtcctatata
     1321 ttatcttgaa ttagttctac cacaattaat ttctactaaa taattataat ttaaacaatc
     1381 attccttact attttcacac tgctttacaa attcaattgg atgttgaatt attttagaag
     1441 tttcttctac agaatatccc tcagataaat atcttctagc tataacttcc atagactctc
     1501 ctatctcaat aatatgctta tctgggtcat aaatacgcac tactctttgt tgccattcat
     1561 gttttagtgg cttatggaca aactctacat ctttataata attgagtttt tcaataaatt
     1621 cgtcaaaatc atcaacctca aaataaagtt ccatattatt tgatttttgt ataactgtat
     1681 ctggattaat ccctgtaagc catgcgaagt cttcttgtat agcaaatcct cctttaaatg
     1741 ttacattttt acctaagtcc aattcaattt cttgctcaaa taattctttg taaaattttt
     1801 ttgatacttc aatatccttc actgctaata atgaaacctg atatttcata aattttcctc
     1861 ctaatttttt atttctattt tttcagtatc ttctattaac atttttttct ctatctcttc
     1921 agtacctttt attaatcctt tatttttact ttttta
//
LOCUS       DAGSWI010000286         1942 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.291, whole genome shotgun sequence.
ACCESSION   DAGSWI010000286 DAGSWI010000000
VERSION     DAGSWI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.291"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            158..1735
                     /locus_tag="MJF80_003933"
     CDS             158..1735
                     /locus_tag="MJF80_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBZ0282510.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIISLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKEDTVNEAFRMVTNKPITVNIKGDCSKTITVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGNIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT          802 a          182 c          368 g          590 t
ORIGIN      
        1 tttatcattt aaataatgaa aaataagttt gtctatattt ttagatagtt ttcttattat
       61 tgatgaaaaa tgtaataatt ttaaactgta aaatagtgta aagttatttt ataatataaa
      121 tatagtaaga ttttaaacaa aataaggggg aataataatg aaagcaccaa aaactatttt
      181 aacaatactg acaatagcgc ttactttaag tagcatttct ataataccat catatgcact
      241 tacagaggaa aaattaatag gtaatggtag atatgaaact gctgtaaaga taagtcaaaa
      301 ggcatacagt tctagttcta atgtagtgtt agttaatgac aattcattag ctgatgcact
      361 ttcagctaca ccatttgcaa aggctaaagg agcaccaata cttttgacag aaagtgataa
      421 gctagatgat aggactgaaa aagagataaa acgtctagga gctaaagata tttatttgat
      481 aggaggtact gctgtactta ataaagatat agagaacaag ttaaaaggta atggacttaa
      541 tgtagaaaga attaatggta aggatagata tgaaacttca ctgattttgg ctaataagct
      601 taaggatata aaagatatta aggaagttgc tgtagtaaat ggtgaaaaag gattgagtga
      661 tgctgttagt gttggagcac cagcagctca aaataagatg cctataattt tgtctagtcc
      721 taaggatgga gtagaagcat ttgataaatt tataagagat gagaaagtta taaaagcata
      781 tgtaattgga ggtacaaatt ctgtttcaag agctgtagaa aagagccttc ctaatgcaga
      841 aagagtgagt ggaaaagata gaaatgagac taatgctaaa gttatagaaa agttttacac
      901 tgatacaaac ttaagtaatt tatatgtaac taaggatggt agtaaaaatg agaatcagct
      961 aatagactcg ttagctgttg gagtattggc tgcaaaaaat gagtctcctg ttgttttggt
     1021 tggtaataaa ttaaacacaa aacaaagaga tatacttagt actaaaaaat taaatactat
     1081 aacacaggta ggtggaaatg gaaatgaaga agcttttgat gaaattataa gtctacaaga
     1141 aaaaactgtg tttgaagcaa aaactgttga ggaattaact gatatgataa atatagcaag
     1201 tcctaatgac attataaact ttaaaccaaa agaggatact gtaaatgaag cttttagaat
     1261 ggtaacaaat aaaccaataa ctgtgaatat aaagggagat tgttcaaaga ctattacagt
     1321 agatatgcct aatggtgaag tgaataacta tgctactttg gtaaatgtta tagttagaaa
     1381 tattggagaa ggtggattta ataatcatga tacaatcact atattgtcag ttcgtgacaa
     1441 aaatggtaga gttatagaaa atactagaaa ttcagatata gatacgttga tgatattagc
     1501 ttctgctaat gatacaaaat tgattaatga tggatatata ggaaaactta tagataattc
     1561 atctaatagt gatataacta ataatggtaa tatagataaa aaagttaatc aggttgagga
     1621 tttagaggct aaggttgatt caattgaaaa agctatagat tctataagtc agaaagtcaa
     1681 taaaatacaa gatatactag ataagcttgg atttctaaag aaatttctta gctaagaata
     1741 tgtatattta agtgaagtgt gtgttgtaat tgttctaaag ttatatagtt aaacaggttt
     1801 atatacaaac agacttatag ataaatatgt agttcaatat atatttgaat aagctttggg
     1861 gaggtaacat ggggattgga attgaatgta acaaaaaaat aaataaaagt attgacaaaa
     1921 ctagaataaa gaaaaataat ct
//
LOCUS       DAGSWI010000287         1895 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.292, whole genome shotgun sequence.
ACCESSION   DAGSWI010000287 DAGSWI010000000
VERSION     DAGSWI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.292"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            584..1693
                     /gene="ugpC"
                     /locus_tag="MJF80_003934"
     CDS             584..1693
                     /gene="ugpC"
                     /locus_tag="MJF80_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBZ0282511.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
     gene            1723..>1895
                     /locus_tag="MJF80_003935"
     CDS             1723..>1895
                     /locus_tag="MJF80_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBZ0282512.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNF"
BASE COUNT          778 a          233 c          236 g          648 t
ORIGIN      
        1 actactgaaa tagaaataat gtataacatg cgatgaaata atttattaca aaataaattt
       61 actactactt tcataaatct ttttaatcct ttcttaatta tttcttttat tctgtttaaa
      121 ttcatattta ttccccctat gtatttttat atttattaaa tcatatttac attatatgat
      181 attacttata tagagtcaat aaattatttc tatataatta atttgaccgg ataaatataa
      241 ttatatataa ctaaatttac tagaaattta attatataca atcaatacaa tcttaattat
      301 attattttat ttttatactc ctcattgtta atctaaacta ttaatataat actatttcct
      361 tataaattaa attgacattt aaatcccatg ttgttgaact aaaaactata tactattata
      421 cataaaactt ttctaaatta aaatacattt tttaaattaa aaaactatta gtctaaattt
      481 ttaatataca tattaaacaa atacatttag tcattttaca catttaatat aataaaaaaa
      541 tttgatacta tataattaaa atttaaatat caaggaggat attatgtcag aagtaatttt
      601 aaaaaatata tcaaaacttt actcaaatgg atttaatgca gtaaaaaata taaatataga
      661 cattaaagat aaagaattta tcgttttagt aggaccatct ggttgtggta aatcgactac
      721 acttagaatg attgctggtc ttgaagaaat ctctgaagga gaactataca ttggagataa
      781 acttgtaaat gatatagaac caaaagatag agatatagct atggtttttc aaaactatgc
      841 tttatatccc cacttatctg tttacgaaaa catggcattt gcacttaaac ttagaaaatt
      901 gcctaaagat gaaatagaca aaaaagttaa agaagctgct aaaatattag acttactacc
      961 tcttttaaat aaaaaaccta aaacattatc tggaggacaa agacaaaggg ttgccttagg
     1021 acgtgcaata gttagaaatc ctaaggtatt cttaatggat gaacctctat ccaacttaga
     1081 tgcaaaactt agaactgcta tgagaacaga gataactaag ctacaccaac agcttggaac
     1141 tacatttatc tatgtaacac acgaccaagt tgaagctatg actatggctg atagaatcgt
     1201 agtaatgaag gatggagtag ttcagcagat tgcaactcca caagatgtat acgattatcc
     1261 agctaatata tttgttgcag gatttattgg agcaccacag atgaatttta ttgatgtaat
     1321 attaatagaa gaaaatgatg aaatttatgc acaaaatgaa catattaagc ttaaattgaa
     1381 taaagaaaaa cactatgatt taataaaaga caattatatt aataatgaag ttgttatagg
     1441 tatcagacca gaagatattc atgtagaaga tacttttatt aaatctaatc cagatacttg
     1501 ttttaaatca agaattgaaa taaaagaact tatgggagca gaaacttatg ctcacttaaa
     1561 actaggcgaa aatacgataa caattagatt tgacagtaaa aacagaatta atgttggtga
     1621 tgacttaaca ctttcagttg acaacagtag agtacacata tttgacaaag aaactacttt
     1681 agctataaga taagaatata atcttatttt taaggagact ttatggaaga gttaaaagat
     1741 tttttagaaa gacagcttaa taaaaaaata aatatctatc catatgaaaa ccaaaagtta
     1801 gatgctagta gccatttagt tactttcaat aatgctattt atgttattga ttttctagat
     1861 aaaaataatc ttgataattt aaaaggcaac tttta
//
LOCUS       DAGSWI010000288         1871 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.293, whole genome shotgun sequence.
ACCESSION   DAGSWI010000288 DAGSWI010000000
VERSION     DAGSWI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.293"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            77..691
                     /locus_tag="MJF80_003936"
     CDS             77..691
                     /locus_tag="MJF80_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBZ0282513.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDGSEYLRKS
                     VGNALRNISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            709..1071
                     /locus_tag="MJF80_003937"
     CDS             709..1071
                     /locus_tag="MJF80_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0282514.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1177..1674
                     /locus_tag="MJF80_003938"
     CDS             1177..1674
                     /locus_tag="MJF80_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBZ0282515.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          708 a          210 c          322 g          631 t
ORIGIN      
        1 ttgtctaaat taaacaaact tttttacaaa taaatacata aaatttaaac taatctattt
       61 attggaggag ataaaattgg aagaagtaat tgcaaaaatt attactatag aaaatggatt
      121 caaagaaatt gaaaacattg ctaaaaaaat tgtaaaaaat aatgactcaa aaaagtgtta
      181 ttatctatca atctacttat ataggtcaga atattatcaa gtaagagaat tggctgtgtt
      241 tatacttggt tttatatcag taactatttc tgaagctttg ttatttttaa aagataatgt
      301 gagtaaagat gaaaactgga gagtacaaga aatactagct aaatcatttg attattattg
      361 cagtgaagta ggatatgaaa aggctcttcc tgttataaaa gaatggctta attctgatag
      421 tccaaacata aaaagggctg tgacagaagg tcttcgaatt tggactagta gagactattt
      481 taaatctaat cctgacgttg ctatatcact actttctagt ttaaaagaag atggtagcga
      541 atacttgaga aagtctgtag gtaatgctct tagaaacata agtaaaaaat atccagattt
      601 agtaaaaaaa gaattagatt catgggatat atcaaataaa aaagttgagt ttgtatataa
      661 gcttgcttca aaatgtatct tggcaaagta aaatggaggt gaacttattt gaaacttcgt
      721 gatgagtata cttgcccatt agaactaaca catgatatga taaaaggcaa atggaaatct
      781 attatattat ggagattacg cataggacca acttccttat caaaacttga aaaggaaatc
      841 aatggtatta atcaaaagat gttacttgaa caacttaaag aattaattga atttggtctt
      901 gtagataaaa agacatatga aggttaccca cttaaagttg aatactcact tacacctatt
      961 cgagggaaac aaatattgga tgctttggct ataatgcaag atataggaat tagctttatg
     1021 ttggataatg gtatggaaga tattcttaag gaaaaaggtg ttttaaagta aatagatacc
     1081 cacaaaaaag taagtaattt accattattt tcaactggta tataattaaa ttatagatat
     1141 attctattaa tttattataa taggaggtaa attattatga aagtaactag tacaggtata
     1201 gtaaatggtg ttatagaaga taaatacggt aaaagaggaa ctcagttcaa tgaaggtggt
     1261 atgcctacat attcactacc tctaaagttt gaagatgctc caaagaatac agtatccttt
     1321 gctgtatttt tagaagataa agactctgtt cctgtatgtg ggtttgcatg gatacattgg
     1381 ttagctgtaa atataacaaa agatgagtta aatgaaaatg aaagtgtaac agctactgat
     1441 tttgtacaag ggactactag ttggcatggc aaactaggag gtcttgacag attagaggcc
     1501 agtgtatatg gtggaatggc tcctccagac agacctcatg tctatgaaat acatgtattt
     1561 gcacttgata ctattcttga ccttgaaaaa ggattctata tgaatgaatt tttcaaagct
     1621 attgaagggc atgttttaga tacatttaca ctaaaaggaa gttattctaa ttaattaggt
     1681 tgactatagc taatcattaa ttaaatgcta catttaattg atgattagct taattgttat
     1741 tagaatattt tctgcacttt atcttaatat atattttagt tgactggtat tttaatttat
     1801 attagattat tatcaattta tataaattga ttttagctta aaacttactt atctatttca
     1861 gtttctatta g
//
LOCUS       DAGSWI010000289         1860 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.294, whole genome shotgun sequence.
ACCESSION   DAGSWI010000289 DAGSWI010000000
VERSION     DAGSWI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.294"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            130..1269
                     /locus_tag="MJF80_003939"
     CDS             130..1269
                     /locus_tag="MJF80_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBZ0282516.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKIIEDKNDLALESYYSSVIEGAFSNRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HIGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKEFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICSKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1295..1483
                     /locus_tag="MJF80_003940"
     CDS             1295..1483
                     /locus_tag="MJF80_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282517.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          790 a          148 c          273 g          649 t
ORIGIN      
        1 tttgcattat ttattaaaat ttattatgct ttaacgttaa attgagtttg ataattataa
       61 aacaaataat caaaaatagt gtaaaaatta atactttttg ttaatattaa tagataaaga
      121 taatctaagg tggtgattaa actgacaata gaagaataca aaaacaaata ctttactgga
      181 aataaaatcg tactttctat aaatagtttt gataataaag aactatacta tcatgaaact
      241 gaaaatatat taaatttaat aaatgaaata gataaattaa catcaagtaa gatatttaaa
      301 ataatagaag ataaaaatga tttggcacta gagtcttatt attctagtgt aatagaaggt
      361 gctttttcaa atagaaagat tgcaaaatct ataattagag gaaagatgaa accaagtaat
      421 aaaagtgaat atatgattta caataatcat agagccttag aatatggatt agacaactta
      481 gataaattat atagtcacaa atttatatat gatttgcatc atatattggg cgaaaactgc
      541 ttggattcag aagaatatga gtatagaact gaaaaagtat atgtatgtga ttcaaaaggt
      601 gagattatac atatagggct tgaacctttg aagatatatg actttatgag taaattaata
      661 gattttatgg aaaatagcaa agtaagtaat ttaattaaaa gtgctataat tcatttttac
      721 tttgtatatg ttcatccttt tagtgatggt aatggaagaa catcaagagc cttatcatat
      781 ctatatttga ttgataaagg atatgatact ttcaaggagt tttcaatatc atatatgata
      841 tctaaaaaca gaactaagta ttataaggct atacttgatg tagaaaacaa aggaaataat
      901 ttaactgtat ttatagagtt tatgctcaag tctataatac aatctattaa tgagatgcgt
      961 aatatgcatg atagaaaatc tttagaaagt atattaaaag aggaattgtt tgaaaatgac
     1021 ataactctat cagcaacaga agaacatata ttaaaatata tctgtagtaa agataactat
     1081 tctatgacat tagaaaatta tataaaaaag aataaaagta gatacttaaa ggctggaata
     1141 aaggaaattg aattagttga ccagttgatg gaagtgttta ataatcttga agagatagag
     1201 attttgtcta aagaaaaaaa tatatataaa gttaatgata agtacttaaa aatgcttgat
     1261 atagactagt ttagataatc tggagaggga atatatgaat aataagaaaa aggcattttt
     1321 gatttctggg gctataaacg taatttcatt aattattgtt ttttcattgt ttcattataa
     1381 tattatagaa aatagtacac aaaatatgat aataactgta atcttaattt ttatttttga
     1441 aatgataaaa ataaaaatca tagagaaata ctataaacta taaacaagaa atatcttttc
     1501 taagagcata ttgtacaata tgctcttaat ttagttatat agatttaata taaataatgt
     1561 attttttgta gatgatttat gtaagattta tgaccaattt gtgtatgatt tgtgttcttt
     1621 aatcatgaca atattatatt gtaaaaaata aatttaataa aaaatgactt ggaatttata
     1681 ttctaggtct tttttatata aaaatttaat aagtttcaaa tgttggtaac tttgacagta
     1741 gaaaacgtct attgagataa cgaggattct aatttagggg gagatatatt agcacttaaa
     1801 gtagtatatg caacagatat aatctttaca aaagtaattt gaattaaaat gtaaatataa
//
LOCUS       DAGSWI010000290         1808 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.295, whole genome shotgun sequence.
ACCESSION   DAGSWI010000290 DAGSWI010000000
VERSION     DAGSWI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.295"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..836
                     /locus_tag="MJF80_003941"
     CDS             <1..836
                     /locus_tag="MJF80_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBZ0282518.1"
                     /translation="FNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLVYT
                     LLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNSCV
                     ILPPCDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDLDC
                     EFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMFNE
                     KYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQVNVYID
                     N"
     gene            1188..1757
                     /locus_tag="MJF80_003942"
     CDS             1188..1757
                     /locus_tag="MJF80_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBZ0282519.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
BASE COUNT          712 a          149 c          301 g          646 t
ORIGIN      
        1 tatttaatga gtcaaaatat aatggtaata ttaataagat aagtttaggc aattttaata
       61 gtatttctat tgtatatagt actgttttag tctctgtttg gattgctatt acgatatttt
      121 atttagttta tactttattg aaatatatta agcttaaaaa tatgataaat gatttgtctt
      181 atgatgttga ggatgataag ataaaaaatc tttatgaaaa tttattaaaa gagcttaaga
      241 taaataaaaa aatacggctt aaatattcat atgaagttga aagtccagta ttttttaatt
      301 cgtgtgtcat tttaccacca tgtgattata cattaaaaga gttaaactgg atatttaggc
      361 atgagttgat gcattttaag agtaaagacc tttatattaa gtatttggtg atatttttaa
      421 aatgtgttta ttggtttaat ccttttgtgt atataatgga gaagtttata gatttggatt
      481 gtgaatttta ttgtgatgaa agagttttga agaattgtac gatagaagag aagcaggatt
      541 atgcattgac aataattagt gcaatgagaa aaggttcaaa tagttcaaat aaatttgtgg
      601 ctggattaca taaggagtca gatattaaaa aaagagtgtt tagtatgttt aatgaaaagt
      661 acagaaatgg tattttgata gcagttattt tatgtttatt atcttctatt acatatttta
      721 aaattgattt aattactact aaatcaatat tttatccact ttcaaaacaa acacctattg
      781 aaaacagaac taattcaaaa cctacatatc aggttaatgt ttatatagat aattaaaaaa
      841 tacaaagttt aacaagagat taatataaat aattttaata agtatctttc acatatgtta
      901 aatttacttt aattatatta aagccaatga attattcttt agaagctcca atgccagtaa
      961 atacaggtaa agaaaaaaat actacaattg tagattaaaa atgttctaat atttaaatag
     1021 atacaaaagt gtaagaaggg taaaaatctg ttataataca ttaaaaacta aatttagaaa
     1081 aatttacata attagaaaaa tgatttgata aaaaataaag ttaaatatta actaagtaaa
     1141 tttgaaaaag aaaaaatata aagtacaaat ttaaaaggag gtaactaatg aaaaagaagt
     1201 tggaagatat gactttagag gagttatgga acttgtttcc tatatttttg actactcaca
     1261 aagaatgttg gaaaaattgg tttaaagaag aacaatatag actaactgga atattgccaa
     1321 tgcaaaaaat tgttagaata agtcatattg gaagtacttc tgtgagtacg atttatgcca
     1381 agcccattgt tgacatactg attgagataa ttcctgatta taatatgaaa gatattaaag
     1441 atttgattga gtcgaatgga tatatttgta tgtctgagag cagtagtagg ctttcactta
     1501 ataaagggta tacagtagat ggatttgaag aacgagtttt tcatttgcat ataaggtatg
     1561 taggtgataa tgatgagttg ttttttagag attacttaat tgaccaccca gatgttgcaa
     1621 aggagtatga aaaacttaag ttggaccttt ggaagaagta tgagcataat cgtgatgctt
     1681 atacagatag taaactagat tttgttaggg agtttactga aaaggcaaag gtgttgtatt
     1741 ctaatagata taattagaaa taggtacaat tagatattta tcttatatat cgtattatat
     1801 tttttaag
//
LOCUS       DAGSWI010000291         1773 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.296, whole genome shotgun sequence.
ACCESSION   DAGSWI010000291 DAGSWI010000000
VERSION     DAGSWI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.296"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(18..584)
                     /locus_tag="MJF80_003943"
     CDS             complement(18..584)
                     /locus_tag="MJF80_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBZ0282520.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            745..1398
                     /locus_tag="MJF80_003944"
     CDS             745..1398
                     /locus_tag="MJF80_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBZ0282521.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(1556..1732)
                     /locus_tag="MJF80_003945"
     CDS             complement(1556..1732)
                     /locus_tag="MJF80_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282522.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT          600 a          222 c          220 g          731 t
ORIGIN      
        1 gtcttacttt attaaaatta ttttttggtt atgtctggaa aatatctgtc tactaatttt
       61 tctgaaatat tttttaattg gtcagccatt gaagtcagac ttaataaggc tatcaataaa
      121 tcatctttat tttctggatt tatactaata ttttcaaaat tagactctat aaagtccggt
      181 atattctttc tttgattttc ttttattaac atgaattttt cgtaacatga aactaaatct
      241 ttttcactga aattatcctt ttcatacaca ccatgtaaga ttcttttaat ttcttgtatt
      301 gtaagtgtat ttttcataga ataaataatt atcatctgta aaatttgttc tttagtatac
      361 ttttttccct taactggttt gattaatccc tctttgctgt aattatttat catggtttta
      421 gtaagaattt tatcactttc aaatctttta ttagcagaaa gcttattgtc aattagagaa
      481 ataatttgat ccatatataa gtcaatagat ggaatatcgt ttgaagttat atctgcatta
      541 tttagtgttt ctagtattat agtattaagt tcattattca tcatatgtta cctccataat
      601 atatataata cactaaatta gaaagtaaaa caatattaag gcttgtaaaa aaaacataaa
      661 gtggtataat taaatatata atatagtaat ggaaactata tttatgaatc tgatttttaa
      721 aatacataat tgtaggagga cgaaatgtat caattttttt taaagggtag agacccaata
      781 agcagtttaa ctcattttat aggtgcttgt ttatctcttt tagcaactat aattttagtt
      841 tttcaatctg ttacattaca ggaaacttct ctcttgatga tagtatctgt atcggtattt
      901 ggactttcac taattgcttt atatagtgca agttcgtatt atcatttttt aaaggggaca
      961 ccagaacaag aattgttttt tagaaaagta gaccatgcaa tgatttacgt attgattgct
     1021 ggctcttata caccaatatg tctgaatttt atggaaaaaa aagaaggcat aatatttgtt
     1081 acagcaatat ggatagttgc atttattggt ataataataa aaatattttg gatggatgca
     1141 cctagatggt tatctaccag tatatattta cttatgggat gggcaattgt ttttgatata
     1201 aatgcattca attcaattcc taaggattgc cttagactat taattatgga aggtgtatct
     1261 tactctatag gtgcaattat ttatataatt aaaaagccta atataagtcc tgagtttgga
     1321 tttcatgaaa tattccatat ttttattatg ataggttctt tatttcactt tttagcagtt
     1381 ttattgtatg ttttatagtt ttaaaagatt tgattaattg atactaaaat tctacaatga
     1441 ataaaatgga actgccttat atagattata tcttcgacaa taagacagtt ccttattata
     1501 aatattaaat tctacttata ataaaatttt gataaaaata gtccattcaa aatgcctatt
     1561 ccataattcc tttaatgtac ttttcattta cgacatatag tttgaattta tgtactataa
     1621 ttatatcctc taaattctct gaaacactaa caattctgac acatttgttt ctaatttcta
     1681 caggtaaatc taaatcattt gttaaattca catagtcatc atatttatac ataaaatcat
     1741 ccctttacat taagatatac ttattatact ata
//
LOCUS       DAGSWI010000292         1717 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.297, whole genome shotgun sequence.
ACCESSION   DAGSWI010000292 DAGSWI010000000
VERSION     DAGSWI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.297"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..166
                     /locus_tag="MJF80_003946"
     rRNA            <1..166
                     /locus_tag="MJF80_003946"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            294..410
                     /gene="rrf"
                     /locus_tag="MJF80_003947"
     rRNA            294..410
                     /gene="rrf"
                     /locus_tag="MJF80_003947"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            418..492
                     /locus_tag="MJF80_003948"
     tRNA            418..492
                     /locus_tag="MJF80_003948"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Asn,seq:gtt)
     gene            498..583
                     /locus_tag="MJF80_003949"
     tRNA            498..583
                     /locus_tag="MJF80_003949"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:532..534,aa:Leu,seq:taa)
     gene            599..674
                     /locus_tag="MJF80_003950"
     tRNA            599..674
                     /locus_tag="MJF80_003950"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:632..634,aa:Met,seq:cat)
     gene            682..756
                     /locus_tag="MJF80_003951"
     tRNA            682..756
                     /locus_tag="MJF80_003951"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:715..717,aa:Glu,seq:ttc)
     gene            771..844
                     /locus_tag="MJF80_003952"
     tRNA            771..844
                     /locus_tag="MJF80_003952"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:803..805,aa:Gly,seq:tcc)
     gene            850..925
                     /locus_tag="MJF80_003953"
     tRNA            850..925
                     /locus_tag="MJF80_003953"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:883..885,aa:Val,seq:tac)
     gene            931..1007
                     /locus_tag="MJF80_003954"
     tRNA            931..1007
                     /locus_tag="MJF80_003954"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:965..967,aa:Asp,seq:gtc)
     gene            1018..1092
                     /locus_tag="MJF80_003955"
     tRNA            1018..1092
                     /locus_tag="MJF80_003955"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1050..1052,aa:Gly,seq:gcc)
     gene            1102..1175
                     /locus_tag="MJF80_003956"
     tRNA            1102..1175
                     /locus_tag="MJF80_003956"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1136..1138,aa:Arg,seq:tct)
BASE COUNT          534 a          311 c          374 g          498 t
ORIGIN      
        1 cggaatggat aagcgctgaa agcatctaag cgcgaagcca acttcaagat aagatttccc
       61 accgcaaggg taagacccca gaaagactat ctggttgata ggtcgaaggt gtaagtgcag
      121 caatgtattt agcttatcga tactaatagg tcgaggactt gaccaatatt tatttgatgt
      181 tcattcatta agatatatgt gtagttttta gagtgctaac tctaaagaaa ctagtattgc
      241 taggttctat aaacttatta aaaacttaat agaaatatta agcataaaga ttatgtggtt
      301 acaatagcag agaggataca cctgttccca ttccgaacac agaagttaag ctctctagcg
      361 ctgatggtac ttggtgggaa actgcctggg agagtaggac gtagccacgt taaatgttcc
      421 aaggtagctc aacggtggag caaccggctg ttaaccggta ggctgtgggt tcgagcccca
      481 cccttggagc caaaaaagcc gaagtggcgg aactggcaga cgcacaggac ttaaaatcct
      541 gcgggactta cctctcgtac cggttcgatt ccggtcttcg gcacttataa aacaatatcg
      601 cggggtggag cagttggcag ctcgtcgggc tcataacccg aaggtcgtag gttcaagtcc
      661 tgcctccgca accattaaaa cggctcgttg gtcaagaggt taagacaccg ccctttcacg
      721 gcggtaacag gggttcaatt cccctacgag tcaccaattc aattaaatat gcgggtgtag
      781 ctcaatggta gagttctggc cttccaagcc agctgtgagg gttcgatccc cttcacccgc
      841 tccaaaaatg ggaccatagc tcagctggga gagcacctgc cttacaagca gggggtcaca
      901 ggttcgagcc ctgttggtcc caccattcgc ggcctggtag ttcagttggt tagaatgcca
      961 gcctgtcacg ctggagatcg agggttcgag ccccttccag gtcgccaaat taaataagcg
     1021 ggaatagttc agtggtagag cgcaaccttg ccaaggttga agtcgcgagt tcgaatctcg
     1081 tttcccgctc cataataact tgggtgcata gctcagctgg atagagcaac gcccttctaa
     1141 ggcgtgtgtc cggggttcga atccctgtgc gctcattaag aaaagtttac tataaattat
     1201 agtaaacttt tatattgttt aaaaagacac aggaaacatg tatattacct gtgtcttttt
     1261 atttataaac tttaaactat gtaaaaatta cagtatattc taatagagtt acagtatatt
     1321 cattaatatt gtaaacatac ggagggtaat gtataatagt ctacaagatg tttatgaagg
     1381 catcagttaa atataataaa tataactaat tagtaagagt tatatacatt atattgtata
     1441 ctttatggta tttatatatt gaaataactt atgaagttat ttataaagag attcatgagc
     1501 atatttttgt ctaaacaata agtataagaa attataaaaa attagagact atagtgaatt
     1561 tacaatttaa tgtaatattt ataagttata ctaaataaat tattatactg tgaaatggat
     1621 atataacttt aggtattgtg tattattttc actgtaggag attatatcta gttatacata
     1681 ataagtttta atgatagttt atagtaagat attaaaa
//
LOCUS       DAGSWI010000293         1635 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.298, whole genome shotgun sequence.
ACCESSION   DAGSWI010000293 DAGSWI010000000
VERSION     DAGSWI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.298"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            68..970
                     /locus_tag="MJF80_003957"
     CDS             68..970
                     /locus_tag="MJF80_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBZ0282523.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKDRGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTAHIF"
BASE COUNT          524 a          163 c          344 g          604 t
ORIGIN      
        1 ttagtatgca aaattctatg aagcaggaac cctgtgactt tagtcatggg aggttcagat
       61 tggaaatgtg ttgccacaag attattatgg attaatagtg attttaggtg ttattcttgg
      121 agttatgggg gcattttaca actggtttac tctatatgtg cagtctttat acaagaagat
      181 tcctaaaatt ggtacctttg gaaaactgtt gattccattt ataatggctg gtattcttgg
      241 gcttgttatg cctgatgttc tggggagtgg acacaattta gttgaagaac ttacaagtca
      301 tgaatatatt tttggaatgg ttgttttgat ttttgttata cgttttatat tttcggctgt
      361 tagttttggg tcaggtgcac cagggggaat attttttcca ttgcttgttt taggagcatt
      421 tataggtgga atatttggta tggtaggtgt taaagttttt ggaatgaatc ccgattatgt
      481 aaataacttt gtgcttttgg ctatggcagg atattttaca gctattgtgc gtgccccttt
      541 gactggtatc atattaattt ttgaaatgac aggttctgtt agtcaaatgc tttcactatc
      601 agtaatttca attgtggcat atattgtggc tacacttatg aagtctaaac ctatttatga
      661 aagtcttctt gagaggttac ttgaaaaaaa tggagaacct gtacataaag ataggggcga
      721 aaagatacta gaccagttct ctgttatgtt tggctctcct gttgcgaaca aggcaattca
      781 ggatgtacag tggcctgaaa attgtctttt ggtagcaatt caaagaggtg gagaagaaat
      841 tattcctaga ggtaagacaa ttcttagacc aagtgatgta attgtgacta tgacagatga
      901 gagagatagt ggtgctgtgt atgataagat ggaaaatttg tgtaaagaac atacggcaca
      961 tattttttag ggataatgat gaatgtttaa taatgataga atatttaatg aagatttgat
     1021 taaaaaattg gtaatttgaa agagagttaa ggaattaata taagaaaatg aaaatgtgat
     1081 atcttagatg taaaagagtt tgaagaaaat aattgtatat atttgttaat gagattatat
     1141 ggactaatgg tatatttata ctgtgctaaa agttgcacta ttgtatgagg aatgggaata
     1201 aagggtttga aaaaataggt ggaagatttt tcagatattc ttattgggat attttaatta
     1261 atatatttta attgcgatat tttgggaaga atagaattta tttataaatg aaaataatta
     1321 aaaggggtta aggttgtatt agcccctttt gcttatagag tttggaaagg gtatcttgat
     1381 agtagttgtt tcatgcttat tatattttat tacatatttt aaaattgacc ttattactcc
     1441 taaatcattg ttatatcaac ttttaaaaca ctttcaagac aatcgcctat tgaaaacaaa
     1501 gttaattaaa aacctacata tcagtttagt attcgtgtag atgattatat aaatataaaa
     1561 gtataagaaa ggtgagtatc tgtggttagt tcatttttaa atgatatctt tagaacaatt
     1621 ttagtaagtt gtatt
//
LOCUS       DAGSWI010000294         1611 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.299, whole genome shotgun sequence.
ACCESSION   DAGSWI010000294 DAGSWI010000000
VERSION     DAGSWI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.299"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(185..1522)
                     /locus_tag="MJF80_003958"
     CDS             complement(185..1522)
                     /locus_tag="MJF80_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBZ0282524.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYKLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPINILFTLFGYFILLIICSKSKLIPIISVVMILYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
BASE COUNT          613 a          201 c          199 g          598 t
ORIGIN      
        1 gtgcctcatc aactaaaaag tttattttga gacacacaca tttttactaa ggtaaataaa
       61 atatcacatt tgattaaatt ttaagttaat gaatatatgt gtaaatattt aattataagt
      121 tattataatt ttacatagta tcaaacaatc taatttgtat tatataagat aatctaattt
      181 tagactaaaa taatctaata taaagaaata aagaaaattc actctcagta taagaaatat
      241 ccaattttcc attgtactta ttaacaattt ctctaatatt ctcaatacca tatccgtgag
      301 aattactatc ttttttatct gaaataaata gttcttgttt tctattgata ggatttacct
      361 tgttgtttgt caataaatag ctaaaatact ccttctcaca acgtgccttt aataccactt
      421 ttctatttga caaatcagga attttcaaag atgcttctat agcgttatct aaagaatttg
      481 aaaaaatagt acataaatct ataggttcta tacccaagtg attgtctata tctatattaa
      541 agtgcacatc aacaccattt tcttctaaac ttgtatgacg aagatttaaa attgagttaa
      601 gagcttggtt tttgcaaaat tgttttccac taagagtacc tatacctatt tttaactgat
      661 taaaatactt tttagctcca gcaatatctt caatttctaa atacgattca atcatttgaa
      721 agtgattatt catatcatgt cgcattttgc gtatttgaat ttgttgattt tctagtaagt
      781 tatagtattt ttcttttagg tgatttgctt gtttttctat cttcatcttt acattttcaa
      841 tcaatggctg taaaagaagt atcacaccga tatttgccag aattgctgac aagatgatta
      901 gataagtgcc ataaggtaag ttgcctaaga taatattctt attactaccc atagggatta
      961 aattagtagg tggcagaata atagcaatta atattgataa aaatgaagat atacatacca
     1021 tatcaataaa aatccatatt ttatttgata agtattgttt tatattgacc aatctattct
     1081 ttaggaaaat atgtattaaa aaccaaaata ataatttaat tagagcagaa attatctgga
     1141 ctattgaaag tattaaaata tactcttttg agtgtataag ttcctcatat atagggttgt
     1201 ttataaaaag aaaattaagc ccaattataa taggataaag tatcatgaca actgatatta
     1261 taggaattaa tttgcttttg ctacatatta taagtagtat aaaatatccg aatagtgtaa
     1321 acaaaatatt tatggggtcg gaaagccata ttatcatttg actaataata gagtagccca
     1381 aaatcaatat gattttgaaa atccaatgat tcctaaagtc tataaaatag gaagtcaact
     1441 tataaagaaa aaaggctatg aaaatattga ttaatattag aataaaattt atgcttgtag
     1501 agattaaaga ataagtgccc atttaatata cctttctatt aattaagatt ctggaaacta
     1561 agttttaaaa aattaaatct taaaaattaa aattctggaa attagatttc a
//
LOCUS       DAGSWI010000295         1579 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.300, whole genome shotgun sequence.
ACCESSION   DAGSWI010000295 DAGSWI010000000
VERSION     DAGSWI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.300"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            59..790
                     /locus_tag="MJF80_003959"
     CDS             59..790
                     /locus_tag="MJF80_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBZ0282525.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            870..1505
                     /locus_tag="MJF80_003960"
     CDS             870..1505
                     /locus_tag="MJF80_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBZ0282526.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNKVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
BASE COUNT          619 a          192 c          210 g          558 t
ORIGIN      
        1 tattatatta attaaatttt tataagataa gttatactta taggagggaa ccatataaat
       61 gaatatttat gctcataggg ggtttagtgg aaagtatcca gaaaatacta ttctagcttt
      121 taaaaaatgt ttggatatgg atatatatgg aatagaactt gatgtccaca gaactaagga
      181 tggaaaaatt gtagttattc atgatgaaaa ggttgataga acttttaatg gacatggttt
      241 tgtaaaagac tttactttaa gaaaattaaa aactctaaac tcatcttttg aaggatatca
      301 aagcaataaa gaatgtaaaa ttccaacttt agaagaagta ttaattctaa tttcacctac
      361 agaccttatt ttaaatatag aactaaaaac tgacaaaata aactatccca atatagaaaa
      421 agacgtttta gagcttattt taaagtataa tatgaaaaat agagttctta tatctagttt
      481 caacagcaat agcttaaaaa actttcataa attagaccct agtataaaaa caggattatt
      541 gtgttattta cctataaaca atgtagtcaa ttttgctaaa tttctgggta atagttacct
      601 tcatccacct ctagttttag taaatgaatc tctaattgaa ctttgtcaca aaaatctact
      661 tggtgtcaat gtatatactg taaatgaaga ggatgatata ctacactgtc taaaactcaa
      721 tgtagatggt atttttacta actatccaga tattgcttca aatcttttac actcaaaaca
      781 atacagctaa ttatacaata gtttgtactc cttgactttt atataattaa tatgtacaat
      841 tttattaaat ttatcttgga ggtataacta tggataactt attaaatgaa cttgaagctt
      901 taaagagtaa tttagataat ctaccaatag aaaaaataga aaattatgat ttgagtaaaa
      961 ctgctttatt tataattgat gttaataatg gctttgcaag acaaggtgca ttatactctc
     1021 ctagagtaga gtctttaata aagccaattg aaatgtttac aaaaaaaata tcaaacaaat
     1081 taaataaagt aatagcattt actgattctc atactcctaa atcaatagaa cttttgagtt
     1141 atcctgttca ctgtttggaa aatgatgtag aaagtgaatt ggttgatgaa cttaagtcga
     1201 tagaaaacct acaaattctt cctaaaaatt ctactaatgg tttttttgca ttagaaaatt
     1261 tggattttga taatatagat aatataatta tagttggcga ttgcacagat atatgtatat
     1321 atcaatttgc aataactcta aaatcatatt ttaatcaaca taatattgaa aaaaatatag
     1381 ttgtacctat gaatttagta gatacttatg atattcctaa cgttcaccct gctgagattt
     1441 taaacttagt atttttcaac agtatgattc aaaatggagt taatgtatta aaagagattc
     1501 gttaagcctc taatacttta catcccatat aatacaatta tcagatattt atttttaata
     1561 aaatttctaa tagaaaaac
//
LOCUS       DAGSWI010000296         1543 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.301, whole genome shotgun sequence.
ACCESSION   DAGSWI010000296 DAGSWI010000000
VERSION     DAGSWI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.301"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            227..1039
                     /locus_tag="MJF80_003961"
     CDS             227..1039
                     /locus_tag="MJF80_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBZ0282527.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1128..1319)
                     /locus_tag="MJF80_003962"
     CDS             complement(1128..1319)
                     /locus_tag="MJF80_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282528.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
BASE COUNT          562 a          183 c          216 g          582 t
ORIGIN      
        1 tttctatata tttatatgac taagttctat aactaaattg aaataatcac tttttcaaga
       61 atgttataat ttttttattg tattcttttt gctatagtct atgaataaat caatagaaaa
      121 tcttctaagt tgcttttaaa taatgacttt atggttcaat tgtactaaaa tagtatatgt
      181 aggttacaaa taatctaata aatattaaag tttggaggta atagatatgg cagattgtaa
      241 ttcatgtcca tcaaagggaa attgtaatag tcaatcaaat tgttctattg aaaataaccc
      301 taacaataaa tttggaaaaa taattggtgt tatgagtggg aagggtggag ttggaaaatc
      361 aactgtaact gctttacttg ctaataagct aaacaaaatg ggatataagg ttggtatatt
      421 agactcagat ataactggac caagtattcc tagacttatg ggtgtaaaaa atgtaaaagc
      481 ttattctgat ggctcttata tttaccctgt agaaaactca aataacataa aagtaatgtc
      541 tataaacctt atgatagatg atgaaaatga acctgttgtt tggagaggac ctttacttgg
      601 aggggttgta aagcaatttt atactgatgt actttgggaa gaattagact atttattaat
      661 agatatgcct cctggtactg gagatgttgc cttaacagtt atgcaatcta ttcctataag
      721 tggaatagtt atggtttcag tacctcaaga tttggtatct atgattgtat caaaagctgt
      781 taatatggct aaaaaaatga acataaatgt ccttggagtt attgaaaata tgagttatat
      841 ccaatgtcct gattgttcta aaaaaatcaa gttatttgaa ggagaatcta ctgaaaagtt
      901 tttagatgat ttagacttag aacttttagg agaacttcct atgactaaag aaattataga
      961 tattactcat aatggagtta cagaaattag tgatgattta gattcaatat tgactaatgt
     1021 tgttgaaaaa attaaataaa taaaaatttt ataattttat atttatcaaa aaaagaggct
     1081 tgtatttatt ttataatcat acaaataaat ataaacctct tttatgttta tctatgtttt
     1141 aaatacacaa aatattcttc tacttcatct ccatttttta cttttactaa gtattcatta
     1201 taatgagcat tatatggctg tgtcattttg cattctatta cattttctat gtcttctaat
     1261 tctaaaagtt ctacatcctt taaaaatttt ctttctatga gttccattaa tctttccata
     1321 atatatcctc acttccccta taattaagat aaactatata tcctcttaat acagagttta
     1381 tctgtttatt atattatatc caatatatta cagtttctaa catcattcat aaattatttt
     1441 aacaaataga gattgatttt ttaataaata tgcctggata aaatgaaaca tagaccaact
     1501 cttagtttta tgtaaagaaa acttaaagaa aactatagat tat
//
LOCUS       DAGSWI010000297         1517 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.302, whole genome shotgun sequence.
ACCESSION   DAGSWI010000297 DAGSWI010000000
VERSION     DAGSWI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.302"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            359..736
                     /locus_tag="MJF80_003963"
     CDS             359..736
                     /locus_tag="MJF80_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBZ0282529.1"
                     /translation="MKEEILIEKLLRVELIVMEYLWKKDTLMPKKKIVKEIKQIYGWH
                     KSTIKILLKRLVDKRYLARYIINFQSYYKIIIDNKEYYTFKKKVLKSSKSIKIMHSLT
                     TIHKNVSKEKLDLLDEYYRNLKE"
     gene            841..1216
                     /locus_tag="MJF80_003964"
                     /pseudo
     CDS             841..1216
                     /locus_tag="MJF80_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          687 a          130 c          197 g          503 t
ORIGIN      
        1 atatattatt acatacctaa aaaactttca taaaaacaaa aataaaatat gcttttagta
       61 acttgttaac ataacttata atttatttta taggcattag cctatatagc tttgtgctac
      121 taaatttctt tgattctagc gatttagtgc tattctacaa ctaaaataat acaatagagc
      181 tatttattgt ttggtagcat tgtttgcaaa ttaaacaaat ttatatatat gaatattatt
      241 ttacgtttaa aaaaacagac taaatatgta gaatatttgg taaaacttac ctctagtttt
      301 tgtcttaaaa agctcctatt attaatttaa gttagaagaa ttaaggaagt gacataaatt
      361 gaaagaagaa atattaatag aaaaattatt aagagtagaa ttaatagtaa tggagtattt
      421 atggaaaaaa gacactctaa tgcctaaaaa aaaaattgta aaagaaataa aacagattta
      481 tggatggcat aaaagtacaa taaaaatact actaaaaagg ctagttgata aaaggtattt
      541 ggctagatat attataaatt ttcaatctta ttataaaata ataatagata ataaagaata
      601 ttatactttt aagaaaaagg tactaaaatc tagtaaaagc ataaaaataa tgcattcact
      661 taccactata cataaaaatg taagtaaaga gaagttagac ctattagatg agtattatag
      721 aaacttaaaa gaatgatata gtaaaaatcg aatgtaatta tttaccttaa ttcctttgtt
      781 tgtaaaactt tattgatata gaatttgaca tatacataaa ttggtaaagg gatgtatact
      841 atgaaaatta gtaaattacc agaagcggaa ttaaaagtta tgagatatat ttgggaatca
      901 gaaaaggtat taatatcaag agaaatcacc aagtctatgg aacaaaaata tgaatggaaa
      961 gatacaacta tatttacagt attaaaaaga ttaataaaga agagagtttt tagaaacgga
     1021 gaaaatagat aagtgtacac actataatgt attagtaaaa gaaaaataat atcagagatt
     1081 ttaaacaaaa gaatttttaa agaatacaca taaaaattca atattaagtt taatgtcagc
     1141 gctatgtagt aaggatgatg aagtagatgg tgatatttta aatttattag aagaatattt
     1201 taagaatttg aaataagaaa atgacaaatt gatacataat aaataaaaag aaggtatgaa
     1261 attataactt tttctacctt cattttgttt attattctga acatgttaag aaaatgaaat
     1321 aggtttgtct aatatttata taaattttag tagaatatgt atagtatata gtagatagta
     1381 tgtataaaaa taaaaactgt tcaataagaa ttaatataca ttaattcagc tgaatccaat
     1441 caaaatactt aaataaaagt aatctgaaaa taacataatt caaatcaatt tattcaaata
     1501 aacctcaatt tatctaa
//
LOCUS       DAGSWI010000298         1513 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.303, whole genome shotgun sequence.
ACCESSION   DAGSWI010000298 DAGSWI010000000
VERSION     DAGSWI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.303"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(298..1320)
                     /locus_tag="MJF80_003965"
     CDS             complement(298..1320)
                     /locus_tag="MJF80_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBZ0282530.1"
                     /translation="MNYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPAIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
BASE COUNT          568 a          282 c          155 g          508 t
ORIGIN      
        1 taaagaaaga aatttaaatc taaattaata agaaatattt caaaaaatca agaatttttc
       61 ttaaagtgat aataattttt gcaaaaatgg aaaaaatatc aaaataaggc tatgattttt
      121 aaagctatag ccttatcagt aaaaatttaa ttatcattta aaaaataaat ttttatttaa
      181 agaatagaca taaatatttc cagactctct caatatgatt tattaaacac taaatgagat
      241 actattttgt atacgttaaa ggatatatta tttaaatata aatgttataa aactatttta
      301 caataaattc catataactc taccatctat atccttaaca ttttggcaac cagtcaaaat
      361 catagtctca cataactcat tcctaacctt ctctataaaa aactccacac catcaagacc
      421 accgccaaaa gaagcagtaa caaaaggtct tcccatcaaa acagcatcag cacccaatcc
      481 aagcatctta actacatcaa caccagtcct aacgccacca tcaacaagta ttgttatctt
      541 tccctttaca gacttagcta tagctggaag tacctcacaa gtaccaggag tataatcaag
      601 tactcttcca ccatgatttg aaacaataat ggcactagca ccagactcta cagccatcaa
      661 agcatcatct acagtcataa ttccttttat aataaaaggt aatctagtgg attcaaccaa
      721 ttctctaatc tcatcaattg ttttaggaga aaaaggattt tcttgaaatt gattattaat
      781 aagtccacaa gcatcaaggt ctacaccaac tgcaaaagca cctgcttctt cagacaatct
      841 aattttttct attatctttg aattattcca aggctttata aatacaattc catttcctct
      901 attatccttc aacacatcta aattatccag taaaaaagta tctacattag tatcgccaac
      961 catagcataa attccagaat tagaacaacc tctaacaaca ggctctatgt attctttttc
     1021 ggaaacttta ccacccatgt tcaatatagt acctgataca ggtgcagcga atataggaga
     1081 actcattttt ctaccaaata attctatgga tgtatctggc tttgaaacat tatgtatgac
     1141 tctcatattt atctttattt tttctagact ttttctattt tcaatgaaag acgaaccact
     1201 tcctttacca cccataccag gaacatctcc agcacaagct aatccattac aaattttaca
     1261 tactttacat gttccattaa agttctctct agctgatttt agtaagtcat tgtaattcat
     1321 acaaaatctc tcctttttat aatattaaaa ttgagtatta ctatattgaa aatatcttaa
     1381 ttgtaaatat agggatgtta ttatgatgag ctaaactaga taatacatat tagatatttt
     1441 ttattgttgt agatagttaa attatatcat attagatagt caaattttga aaaaaactaa
     1501 cttatttaag tat
//
LOCUS       DAGSWI010000299         1504 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.304, whole genome shotgun sequence.
ACCESSION   DAGSWI010000299 DAGSWI010000000
VERSION     DAGSWI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.304"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1177)
                     /locus_tag="MJF80_003966"
     CDS             complement(<1..1177)
                     /locus_tag="MJF80_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBZ0282531.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECPPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGEIGPTGVTGPTGNTGATGSIGPTGVTGPTGNTGVTGSIGPTGVTGPTGNTGVTGS
                     IGPTGATGPTGNTGEIGPTGATGPTGVTGSIGPTGSIGPTGVTGPTGVTGPTGVTGSI
                     GPTGEIGPTGATGPTGATGPTGPTGVTGSIGPTGATGPTGNTGVTGEIGPTGVTGPTG
                     "
BASE COUNT          365 a          321 c          210 g          608 t
ORIGIN      
        1 ctcccgttgg gcctgttact cccgttgggc ctatttctcc tgtcactcct gtatttcctg
       61 tcggtcctgt tgctcccgtt ggccctatac ttcctgtcac tcctgttggg cctgttggac
      121 cggttgctcc cgttgggcct gttgctcctg ttggccctat ttctcccgtt ggtcctatac
      181 ttcctgtcac tcctgttggg cctgttactc ctgttggacc tgttactcct gttgggccta
      241 tacttcctgt tgggcctata cttcctgtca ctcctgttgg acctgttgct cccgttggtc
      301 ctatttctcc tgtatttcct gttggacctg ttgctcctgt tggtcctata cttcctgtca
      361 ctcccgtatt tcctgtcgga cctgttactc ctgttggtcc tatacttcct gtcactcccg
      421 tatttcctgt cggacctgtt actcctgttg gtcctatact tcctgtcgct cctgtatttc
      481 ctgttggtcc tgttactcct gttggcccta tttctcctgt cgctcctgta cttccggttg
      541 ggcctgttac tcccgttggt cctgtacttc ctgtcgctcc tcttggtccc attggacctg
      601 taattctaat tggaggacac tcacatttag gataacaatt atctataatt ctatcttcaa
      661 atgttacaag tatatacggt ttgttacaac ctctactaga tgtaaattca agaatctgat
      721 aagggtaatt taaagataaa gctaacccat aattaggata tttattcatt gcccatgctt
      781 ttatagttcc cgtaatattt aaacaaatat agttggatgt atcacttatc ccaattggta
      841 aatatcccct aatattttcc atagacactc tatcattcca tgttatagtt cgtaaattat
      901 aatattcact tattctttta atttcaatat ctactttttc tttaaaaata tgattcttat
      961 ttgaatcaat atgaataaat aactctgctt ttaatatact tttgtctttt aatatatctg
     1021 gtattctcac tttaaaaaaa gtaatcaact tttcttttac aaaactattt gaacatttac
     1081 ttcctattaa gcaataatct aaattactaa agtttttatc tggatatttt ttagatataa
     1141 aagtatcatc atttaaataa agtataatat ttctcattta taatccctcc attttgggtc
     1201 tatatcatac tatgtttaaa atgcatgctt agttacaata caattaaaat ttatagtatt
     1261 aaatttttat ataataatgc tgatttctac aaaaaaagaa catgtcatta gaaatttcta
     1321 atagcatgtt ctttttcatt attatatatt acactttaaa ttattccttg actggcttag
     1381 ctggtgtaaa ctaaaccagt ctttctaaaa tattaagcac ttttttctta ttatgttttt
     1441 taggatttat tgtctatatt attgtaaacc aaagttataa aattgtaaat agaatatatt
     1501 acca
//
LOCUS       DAGSWI010000300         1456 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.305, whole genome shotgun sequence.
ACCESSION   DAGSWI010000300 DAGSWI010000000
VERSION     DAGSWI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.305"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..1359
                     /locus_tag="MJF80_003967"
     CDS             <1..1359
                     /locus_tag="MJF80_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBZ0282532.1"
                     /translation="ISSVEKIQGKNKYEIAGKIADKNAYKTAILINTSNSIADGLSAS
                     GLAGALNAPILLTEKNTIPTETSARLKNVSKVYIIGGTYSISTSVENSLKSKKMKVVR
                     IKGNDRIKTSYNVAKEINSIKKVNTVMLTNAYKGEADAISIASVAARDKAPIILTNGQ
                     SIPFSTSGLKSYVIGGTASMSTTLVNNTKSTRLGGSTRFETNKAIINKFYKDAREFYI
                     AGAYELTNALVGSSLSKHGPMVLVNDGSNKSILKNAKKITSIGYIDSNIVQQCLNITN
                     GIGDINTGVVKNVKPTTKTIKDGMYKVGKDISAGEYLITSNSGSYASYYEVTSDSTGN
                     ADSILSNDIFSGTRYITLKNGQYIKIEDSTMTLAKYAKAQKAKNGKFGNGMYKIGLEI
                     PAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTNDTFSGRRYITVEEGQYLILNDCYLIE
                     NE"
BASE COUNT          597 a          154 c          253 g          452 t
ORIGIN      
        1 atatcatctg tagaaaaaat acaaggaaaa aataagtatg aaatcgcagg taaaatagca
       61 gataaaaatg catataaaac tgctatatta attaatacta gtaactctat tgcagatgga
      121 ttaagtgcaa gtggactagc aggagcttta aatgccccta tactattaac tgaaaaaaat
      181 actataccaa ctgagacatc tgcaagatta aaaaatgtat caaaagttta tataattgga
      241 ggaacatatt ctattagtac atctgtcgaa aattcattaa aatctaagaa aatgaaagtt
      301 gtaagaatta agggaaatga cagaataaaa acaagttata atgttgcaaa agaaataaat
      361 tcaataaaaa aagtcaatac tgttatgcta accaatgcat ataaaggaga agcagatgct
      421 ataagtattg catctgtagc agcaagagat aaagcaccaa taattcttac taatggacaa
      481 agtatacctt tttcaactag tggtttaaag tcttatgtaa taggaggaac agcttcaatg
      541 agtaccactt tagtaaacaa tactaaatct actagactag gggggagtac tcgttttgaa
      601 actaataaag caataataaa taagttttat aaggatgcta gagagtttta tatagctgga
      661 gcttatgaat taacaaatgc acttgtagga tcttctttat caaaacatgg tccaatggtt
      721 ttagttaatg atggtagcaa taaaagcatt ttaaaaaatg ctaagaagat aacttctata
      781 ggatatatag attcaaatat agtacaacag tgtttaaata taacaaatgg aattggagat
      841 attaatactg gggttgtaaa aaatgttaaa cctactacaa agactataaa agatggaatg
      901 tataaggttg gaaaagatat ctcagcaggt gaatacttaa taacttcaaa ctcaggaagt
      961 tatgcttctt attatgaagt tacaagtgat agtacaggta atgctgatag tatactttca
     1021 aatgatattt ttagtgggac tagatatatc accttaaaga atggtcaata tattaaaatt
     1081 gaggattcaa caatgacatt agcaaagtat gctaaagccc agaaggccaa gaatggtaag
     1141 tttggaaatg gtatgtataa aattggatta gaaattcctg ctggagaata tattataatg
     1201 tcaaattctt ctgatgcata ttatgaagtt agaaatgata gcttaggtaa tgcagaggga
     1261 atagttacta atgatacttt ttcaggaaga agatatataa cggttgagga aggtcaatat
     1321 ttaatattaa atgattgtta tttaattgaa aatgaataga tgtaattgta tttaatagta
     1381 aatcatatat tttatttaag gaaaggtgtt ttaagtttta atttaaaaca tctttttatt
     1441 attataataa ttatat
//
LOCUS       DAGSWI010000301         1337 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.306, whole genome shotgun sequence.
ACCESSION   DAGSWI010000301 DAGSWI010000000
VERSION     DAGSWI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.306"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            27..101
                     /locus_tag="MJF80_003968"
     tRNA            27..101
                     /locus_tag="MJF80_003968"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="MJF80_003969"
     tRNA            116..200
                     /locus_tag="MJF80_003969"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="MJF80_003970"
     tRNA            210..293
                     /locus_tag="MJF80_003970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="MJF80_003971"
     tRNA            323..399
                     /locus_tag="MJF80_003971"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="MJF80_003972"
     tRNA            407..482
                     /locus_tag="MJF80_003972"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="MJF80_003973"
     tRNA            571..659
                     /locus_tag="MJF80_003973"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="MJF80_003974"
     tRNA            663..738
                     /locus_tag="MJF80_003974"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="MJF80_003975"
     tRNA            745..818
                     /locus_tag="MJF80_003975"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="MJF80_003976"
     tRNA            833..909
                     /locus_tag="MJF80_003976"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="MJF80_003977"
     tRNA            933..1009
                     /locus_tag="MJF80_003977"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="MJF80_003978"
     tRNA            1017..1093
                     /locus_tag="MJF80_003978"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="MJF80_003979"
     tRNA            1102..1177
                     /locus_tag="MJF80_003979"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="MJF80_003980"
     tRNA            1185..1258
                     /locus_tag="MJF80_003980"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="MJF80_003981"
     tRNA            1265..1336
                     /locus_tag="MJF80_003981"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          321 a          309 c          355 g          352 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatataaatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAGSWI010000302         1322 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.307, whole genome shotgun sequence.
ACCESSION   DAGSWI010000302 DAGSWI010000000
VERSION     DAGSWI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.307"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(12..>1322)
                     /locus_tag="MJF80_003982"
     CDS             complement(12..>1322)
                     /locus_tag="MJF80_003982"
                     /inference="COORDINATES: protein motif:HMM:NF016800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBZ0282533.1"
                     /translation="ILAIGVILSSVFSDYKDIALFGNPDRFEGALAWFCYIVIFFVLY
                     NIKIDVKDLKLFYFGLFPFLVINLFLGLAQFFEIDVLQSKILTTLIGGGTLSGTFWTT
                     LYHFNIMSGIASVIFCASLVYMLFEKDIKKKIVPLFGAIMSFTIILISNSISGFLTSI
                     LLFPIILVLSWRFVNKKELCVWTIIMVIISSIIYIILNRHNPNVYQETFAMFEKLNQA
                     SIFIVPILIVTFILLIIIMKFINKKKFFNFVTVCSIVIVSAGLLFFSYTLNKEVSILE
                     SNPNANITRIEDSKLFNIINGMSTDRLNIWTKVMNFINERPILGNGFDTFPYKFISTD
                     KNGALSTYGEVIDKPHSWYMSVAYGSGIIGIIGLVGIIIYLTKEVFYSCVDKNNDKFL
                     YIFFIGFLAYSIQGLFNDSLAGSSIISWVFAGLTANRIFTIKSK"
BASE COUNT          545 a          199 c          116 g          462 t
ORIGIN      
        1 atttttaaaa tttatttaga ctttattgta aatattctat tagcagttaa accagcaaaa
       61 acccaactta ttattgaaga tcctgcaagg ctatcattaa ataacccttg aatagaatat
      121 gctaaaaagc caataaaaaa tatatataga aatttatcat tatttttatc cacacaacta
      181 tagaaaactt ctttagttaa atatattatt atacccacta aaccaattat accaattata
      241 ccacttccat atgcaacact catataccag ctatgtggtt tatctataac ctctccatat
      301 gtacttaaag caccattttt atctgtacta atgaatttat atggaaaagt atcaaatcca
      361 tttcccaata taggtctttc atttataaaa ttcataactt tagtccatat gtttaaacga
      421 tctgtactca taccatttat tatattaaat aatttagaat cttctattct agtaatatta
      481 gcatttggat tgctttctaa aatactgact tctttattta gtgtatatga aaaaaataat
      541 aatccagctg agactataac aatagaacat acagtaacaa aattaaaaaa tttcttttta
      601 tttataaatt tcattataat aatcaacaat ataaatgtta ctatcaatat aggaactata
      661 aatattgatg cttggtttaa tttttcaaac atagcaaatg tttcttgata aacatttggg
      721 ttatgtctat ttaatataat atatataatg gaactaatta taaccataat aattgtccat
      781 acacataatt cttttttatt cacaaatctc caagataaaa ctaatataat tgggaataat
      841 aatatagatg ttaaaaaacc acttatagaa ttcgatataa gaattattgt aaatgacata
      901 attgctccaa ataatggtac tatttttttc ttaatatctt tttcaaataa catatatact
      961 aagctagcac aaaaaatgac agatgctatc ccactcatga tattaaaatg gtataaagtt
     1021 gtccaaaatg ttccagataa agttcctcca ccaataagag tagttaaaat tttagattgt
     1081 aaaacatcaa tttcaaaaaa ttgagctaat cctaagaata aatttataac taaaaaagga
     1141 aacaatccaa aataaaataa tttcaaatct tttacatcta tcttaatatt atacaacaca
     1201 aaaaatataa ctatatagca aaaccaagcc aaagctcctt caaatctatc aggatttcca
     1261 aataaagcga tatctttata atcagaaaaa acagacgata atattactcc tattgctaag
     1321 at
//
LOCUS       DAGSWI010000303         1304 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.308, whole genome shotgun sequence.
ACCESSION   DAGSWI010000303 DAGSWI010000000
VERSION     DAGSWI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.308"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            240..1067
                     /locus_tag="MJF80_003983"
     CDS             240..1067
                     /locus_tag="MJF80_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBZ0282534.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGSGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
BASE COUNT          500 a          137 c          207 g          460 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatgt
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggaaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 gtggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttagtaatta ttctcatatc ttat
//
LOCUS       DAGSWI010000304         1304 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.309, whole genome shotgun sequence.
ACCESSION   DAGSWI010000304 DAGSWI010000000
VERSION     DAGSWI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.309"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..986
                     /locus_tag="MJF80_003984"
     CDS             <1..986
                     /locus_tag="MJF80_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBZ0282535.1"
                     /translation="FLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYT
                     WIITVVVYLIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSY
                     EVETPAFFNSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPF
                     IYIMDKHIDLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQS
                     DIKKRVMNMFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKGMEEKDVTNS
                     AIVTMIEFTYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGIEYYDSLLND"
BASE COUNT          546 a          113 c          178 g          467 t
ORIGIN      
        1 tatttttacc atttacatat tatactttta caataaatat cttgagaagt aaaaaacaca
       61 ttaatattaa taacataaat ttagagggat ttaataatgt ttcaactata agtaataata
      121 ttatattgta cacttggatt ataactgtag tagtgtattt aatttacact atatttaaat
      181 atataaaact taaaaattta ataaatgact tatcttatga tgttgatgat agagagattg
      241 taaatcttta taaaaatata ttagaagaat ttaatataac aaaagatata aaactcaaat
      301 actcttatga agttgaaaca ccagcatttt ttaattcatg tgtacttttg ccaccacatg
      361 agtataaatt gaaagagtta gactggattt ttagacatga gcttatgcat tttaagagta
      421 gggaccttta tttaaaatat gtaatcctgt ttttaaaaac agtatattgg tttaatccat
      481 ttatatatat aatggataaa catatagatt tagattgtga actttattgt gatgaaagag
      541 ttttaaaaaa tcgaaattca gaggaaaaaa aggattatgc tttaacaatt ctaaatgcaa
      601 tgagaaaagg ttcaaatact tcaaataaat ttatagctgg actccataag caatctgata
      661 ttaaaaagag agttatgaat atgtttaatg aaaagtacaa gaatggtata ttaatggcat
      721 taactttatg tttattatct tctattactt ttttgaaggt taattcaacg agtattgtta
      781 atcttaatat attgccaaaa ggaatggaag aaaaagatgt tactaatagt gcaattgtta
      841 caatgataga atttacatat gcagaagctc ctaaaagtat gagagaagaa cataaaataa
      901 attgtgaatt cttaggttta atacctaaag actctgataa aataggtgtt ggtatagaat
      961 attatgacag tctattgaat gattaaataa atattatttt aacagatatt aattataaag
     1021 ttttgaatag taatttgttg agaaataaat atatccttat aaaaatagat acttatatac
     1081 caagaataaa aaatagttaa gatagtacac aaggatatac ttataaagaa cttaaataag
     1141 tgaaataaat tattaattat aaggtttata attttaaaaa attagataaa ttcaggtgta
     1201 aaatactata aaacacaatt cggaaaagtg tatgttgaac ttcatttaaa atggaatttc
     1261 aaagttgcta tagtaagtag tttaagcatt cttcttatat taat
//
LOCUS       DAGSWI010000305         1273 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.310, whole genome shotgun sequence.
ACCESSION   DAGSWI010000305 DAGSWI010000000
VERSION     DAGSWI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.310"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(104..1144)
                     /locus_tag="MJF80_003985"
     CDS             complement(104..1144)
                     /locus_tag="MJF80_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBZ0282536.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
BASE COUNT          397 a          240 c          129 g          507 t
ORIGIN      
        1 aaaatgctat tacacaaaaa aacacttaga aaatagtttt ccaagtgttt tttatcaatt
       61 ttataatttc aaattttaga attgagaata aaaagcctaa ttattataca tctatttttc
      121 tgtacaatgt agctagtgat attccaagtt ttttggcagc tagtttttta gacttagtat
      181 catttccgta aaactttaac actttcttta tataggctct ttcaaactct tcaagtgtaa
      241 gtatgtcttg ttccatactt atctcatagt cactttcctc aagtaacttt atatttatat
      301 tatttaactt aaaatattca agtatctttc tatttattat tcctttatgt attattccat
      361 cttcctcaag aaggtttaat gccagttcta tagtattttc caactctcta atatttcctg
      421 gccaaggata cttaagcaac atttgttcca catctggctc tatatggtac acgtttttct
      481 tcatctttct tgaatatttg tctataaaat tgtttattaa tatttgtata tcgtctcctc
      541 tttctctaag tggaggcact tctaaaggta ttacattcaa tctatagtat aagtcttctc
      601 taaatttatt ttccttaacc aactcaaata aatctctatt agtagctgat atgattctaa
      661 catctatatc aattagttca tttgaaccta ttctcgtaaa cttcttttcc tgtaataccc
      721 tcaataactt tgattgtagt tgaattggca tatctcctat ctcatctaaa aatacaaccc
      781 ctccattggc caattcaaac ttaccaattt taccttttga atctgctcca ctaaaagctc
      841 cttttgtata tccaaaaagt tcactttcta gtaaaggttc tggtatagca gcacagttta
      901 tagcaacgaa tgttttattc cctctgtttc ctgcgagatg aatggctctt gctattaatt
      961 cttttccagt gccactttct ccagttatag ctacagtaga atcactattt ccaattttac
     1021 aaattctatt cttaagagca cgcattacat cagactctcc aattatggag tctactgttg
     1081 ttgttcccca aatattatat acttccattc caccatacat tactgaatca ctgtgtttgt
     1141 ccataaaata tcaactccaa ataagtattc tatataataa tcattcacat tatctattta
     1201 attatatcat aacagaaaag cttagttata atttaataat attattttaa aacaaaactt
     1261 aataaaaagt ata
//
LOCUS       DAGSWI010000306         1235 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.311, whole genome shotgun sequence.
ACCESSION   DAGSWI010000306 DAGSWI010000000
VERSION     DAGSWI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.311"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..269
                     /locus_tag="MJF80_003986"
     CDS             <1..269
                     /locus_tag="MJF80_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBZ0282537.1"
                     /translation="LISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYK
                     NEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            378..1148
                     /locus_tag="MJF80_003987"
                     /pseudo
     CDS             378..1148
                     /locus_tag="MJF80_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          484 a          149 c          187 g          415 t
ORIGIN      
        1 gcttaatttc tgatgtgtca tggtcagagt ttattcgtca attagaatac aaggctaatt
       61 ggcatggtag acaaattgta aaggtgggta aattctttgc aagttcacaa atatgcaata
      121 aatgtgggta taaaaacgag gaagtgaagg acttaaatat aagagaatgg atttgtccta
      181 gttgtaatga aactcacgat agagatataa atgcaagtat aaatatatta aaagaaggat
      241 taagactaat aacaattcaa aataaataac ttatgtaaga accgtaggaa ctacggggat
      301 agcctgtgga gaatttattt tagtatgcaa aattctgtga agcaggaacc ctgtgacttt
      361 agtcatggga ggttcaggat agtggtagtt atgtagcaaa tcattggcat aactcattag
      421 aaataattta tataacttct ggtgatttac aaattaacat ggagggttac acttataatt
      481 taaaagctaa tgagtgtatg ttcattaatt ctggaattat tcactcaact agatgtattt
      541 atcaaaatac ctctatatta ttgcaagtac cattgtcttt tctaaacaaa tatatccctg
      601 attttaaaaa ttgctatttt gactttaagg ttaatgccaa tgacaataac tataaaaaaa
      661 attcaagtaa agttaaactt atattagaaa acatgagaga aattaaactt agttctcctc
      721 ctgcagctaa tctgctcttt acaagcttat tatttgaact tttatttgaa ttatatacaa
      781 actttaaaat atctgtaggt aataaaaata tgaaaaaaac agtacttgat ttgtcaaaat
      841 ttgagccagt tttagaatat acaaagatta attacaaaat gcctatttct attaacaaaa
      901 tcgctaaggt agcacactta caaccagaat atttttgtag aaaatttaaa cagtatatgg
      961 gacaaactta cttagagtat ttaaatgatg tgcgtatttc tcatatttat aaagatttaa
     1021 ttaacacaaa tgatacctta tgcagtatat tagaagctca tggatttact aatacaaaat
     1081 tattttacag aatttttaag gaaaaattta aatgcactcc taaacatata agaaaaaaat
     1141 ctacctaaaa tatgaggtta tatgttattt atctttataa taaaaaaagt ttaccttaaa
     1201 atttgattag aataatcaat tttgaggtaa actct
//
LOCUS       DAGSWI010000307         1226 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.312, whole genome shotgun sequence.
ACCESSION   DAGSWI010000307 DAGSWI010000000
VERSION     DAGSWI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.312"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..>1226
                     /locus_tag="MJF80_003988"
     CDS             <1..>1226
                     /locus_tag="MJF80_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBZ0282538.1"
                     /translation="LGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQRGEP
                     ALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFGSEE
                     GNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFIQNY
                     SKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSATAI
                     RTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDTLKE
                     YFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDMQLI
                     KNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISYDIK
                     ATNIYNSIYYKNNKDL"
BASE COUNT          525 a          163 c          147 g          391 t
ORIGIN      
        1 acttggatta atagttgaat ataacccatt tcataatgga cacatatatc atttatctaa
       61 atctatagaa aaaacaaatg caacacatac tgtagcaata atgagtggaa attttctaca
      121 aagaggcgaa ccagctttat ttgataaata cactagagca aaaattgctg tcgaaaatgg
      181 tgtagactta gttatagaat taccaacttt atttgcttgt caaagtgctg aaattttttc
      241 tcatggtgct ataacaattt taaattcact aaactgcata aattctatat gttttggtag
      301 tgaagaagga aatacagaaa ttctttataa tatagctgaa attttagcaa atgaaccaaa
      361 tgaatttaag ttacttttaa aaaaatattt agatgatggt ctgttatttc caacagctag
      421 agctaatgca ctttttgatt atatacatca agacaaaaaa aataatttta tacaaaatta
      481 ttcaaaggaa caattatcaa aaacattaaa ttcatcaaac aacatactgg gtatagaata
      541 cataaaaaac ctcatccatt taaacagtag tataaaacca tttactatct ccagggtcca
      601 atcagaatat aattccgaag aaattgagag tgatatttgt tctgctactg ctataagaac
      661 ttcacttaaa gaaaatacag acctttttca tttaaaaaat gttattccag aaaaaactta
      721 taacatccta aatgaaaaaa tagataatgg ttttttacct atgtttgatg acttcttctt
      781 tgatatttta aaaacaatta tacttagaga tatggacact ttaaaagagt attttgaggt
      841 aaatgaaggc atagaaaaca gaatctataa agatatcttt acatctgaat ttctacatga
      901 actacaatta gcaattaaat ccaagcgata cactttaacc aaaatcaaac gaactctaaa
      961 caatatattg ctaggtatta aaaaaagcga catgcaactt ataaaaaaca tagatactat
     1021 accttatata agaatacttg cttttaatga taaaggtaga gaaattctaa aagagataaa
     1081 aaataaatct gaaataaaaa taataaacaa gttttcaaat atatcatttt cattagatga
     1141 tacgattttt aaaacactca taagttatga tataaaagct acaaatatat ataattcaat
     1201 atattataaa aacaataaag atttag
//
LOCUS       DAGSWI010000308         1156 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.313, whole genome shotgun sequence.
ACCESSION   DAGSWI010000308 DAGSWI010000000
VERSION     DAGSWI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.313"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            536..1003
                     /locus_tag="MJF80_003989"
     CDS             536..1003
                     /locus_tag="MJF80_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBZ0282539.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSMDEVAKRMDVTVEYIESVLE"
BASE COUNT          465 a           72 c          173 g          446 t
ORIGIN      
        1 aaatataaaa aataaattta tttaaaatta aaattttatt tattattaaa gagtaaatat
       61 aattattagt aatatatata gtatttgtaa caaaatatca ataattagaa agattatgaa
      121 aactaaatta gaagaactaa aaactatatt tttattaatt agaatatttg aataatctct
      181 ttaatcatat aaaatgcata tacttgtaat atttaaaggt gatattaata ttattagtgt
      241 atattaatat tcccttgaaa ttaaaaaaac atagatattt cagtaatcta aaatagtcta
      301 caaaaataat agttttatca aaattaataa gaatacatat agatttaaac ctaaaagtat
      361 gaacatgtct gttttttaca agattaaaat ttatattata tgataaaatt aaattaactt
      421 aactatttca ataagactta taattttttt ctttatacta gaccttattg aaacctttat
      481 agtttaattc taattatata ttataaataa agtatgtgta tattaaggag gtaatatgaa
      541 gtttttaaat gttttaatag tagttgaaga tattgagaaa tcaaaaaagt tttattatga
      601 tgttttgggg ttaaaggtaa tctgtgattt tggagaaaat gttgtattag aaggtaatat
      661 atcattacaa gaaaaaaagc tttggttaga atttattaat aagagtgaca gtgaagttaa
      721 gttcaatgga aatgatgctg agttatattt tgaggaagat aattttgata cttttgttga
      781 aagattgagt acaatgaaag atatagatta tgttcatcta gctatagagc ataggtgggg
      841 tcaaagagca attagatttt atgatttaga tgggcatatt attgaggttg gagaaactat
      901 gtcatctgtg tgtagacgtt ttttagatag tggtctttca atggatgaag tagctaaaag
      961 aatggatgta actgtcgaat atattgaatc agttttagaa tagatggatt atattaaagt
     1021 aaaattgagg tgaaggctgt aagtaaactt gtgtgaaaac aaatttgctt acagttttta
     1081 tttttctaaa ataaagaata taatacttgt ttagctttat ttgagttttt aaaaatatta
     1141 tgatatttct tatata
//
LOCUS       DAGSWI010000309         1125 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.314, whole genome shotgun sequence.
ACCESSION   DAGSWI010000309 DAGSWI010000000
VERSION     DAGSWI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.314"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            41..298
                     /locus_tag="MJF80_003990"
     CDS             41..298
                     /locus_tag="MJF80_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282540.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(408..1088)
                     /locus_tag="MJF80_003991"
     CDS             complement(408..1088)
                     /locus_tag="MJF80_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBZ0282541.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          461 a          142 c          130 g          392 t
ORIGIN      
        1 attagaaatt aaaatgaatt aagtaaatgg aggaattgat atgataataa ttagaagtca
       61 agataaaact aatttaatgc agataaacca aattaaaata gatggtagtc aagtttatgc
      121 agtatttgaa agtaaaattg atactgtaaa aataggagat tatgaaaata atacaagagc
      181 tatacaagtg ttggacaata tacaaaactt cattgaaaat ggaacaaaat atgattatat
      241 aacatcaaat aaagtcagat acaatgttaa taaaatattt cagatgccag ctaaatagtg
      301 agtgtgcatt ttaatttaat aactaaagcc cactttcttt tgtagtatga aagagggctt
      361 tacatatttg taaatatcga agtacactta ttttatcaat aaattattta ataaggtata
      421 atggaccaat taaacaataa aaattctcct aaaacagcaa gtattaaata tataatagct
      481 tcatttttgt aaagagaggt aatttttttg cttatgtcat catcaattgt aatagcaatc
      541 tcatcttgaa taatattttt atatatagat gggaaaatat cttttttctt tataagatta
      601 aataaattat ttacacctac ccatataaaa tatcctaaaa cagcacctaa tgtgttcatc
      661 attaaatcat caatatctgt agctcttaag ttaaacaatt gacaaagttc aattgctaat
      721 gaaaaaccta atccagttaa tgaaacattg ataatatttc taaaattttt ccaaattaaa
      781 ggtagtaaaa atcctaaagg cataaacata attatattta aaatatatgt taagatgcca
      841 actgagtcaa aaggtattag atttatttca tcaattctaa aaagcccact ataccttcct
      901 atatcccata tgctaccaat tccagcttta ctaagtgcta gatagatata taataaaaaa
      961 atatataccc atactaaatg tatcattaca ttcttttcaa ctttcaaatt ttttcgtttt
     1021 ataacgacta tttggtatat taagcatggt aatataacgc aaataaatga ataaaatgtc
     1081 catatcattt tattttgtcc cctttcaaat ttatgttttt agttt
//
LOCUS       DAGSWI010000310         1117 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.315, whole genome shotgun sequence.
ACCESSION   DAGSWI010000310 DAGSWI010000000
VERSION     DAGSWI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.315"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            176..889
                     /gene="rgbR"
                     /locus_tag="MJF80_003992"
     CDS             176..889
                     /gene="rgbR"
                     /locus_tag="MJF80_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBZ0282542.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNAAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
BASE COUNT          494 a           76 c          171 g          376 t
ORIGIN      
        1 attatagtaa tttctgtctt taaattgaaa tatcaaataa tagttgtgtg ggattgagta
       61 ttgataaaaa taatttaaaa atgttatcct aaagtatcag atatacaaaa ttatctttaa
      121 atatattata aatattttaa ttgtattaac tagaaattat gtaaggaggc attttatgat
      181 taagatagca gtttgtgaag atgaaaaaga aacacaactt ttaatagaag attaccttga
      241 gaatatatta aaagatataa gtatagaata tgagatacaa aagtatatat ctggagaaga
      301 gttactggaa agtaatttaa aagatataga tatattactt cttgatataa aaatggaaaa
      361 attaaatgga atggatactg caagaaagat tagagaagta gacaatgaaa tggaaataat
      421 atttgtaact tcattaatag attatgtgca agaaggttat gaggtaagag cttataggta
      481 tttactaaag ccaatagagt tggaagaact aaaaaaacat gtgctaactt gtatcaaaga
      541 tattgaaata aataaggaaa gtcatattac aataaaaaat aagtctaata catataagat
      601 ttatttaaat gaaataaaat atatagaagt tcaaaaaaaa gatatgctaa tacacacaat
      661 aaacaagaat tttgatataa aatatagttt aggtaaaata gaaaaagaat taaatccata
      721 taaatttata agatgccata aaagttttat agtaaattta agatatgttg aaaatataaa
      781 gcctaatgct gcaatactgg aaagtggaga agaggtacct attagtagat atagatataa
      841 agaagttaag gagaaattct taaaatttct tggtgataca atatgctaaa ctttatgact
      901 gagctattag tagttttatc tatatttata cagttttcta taatgaacgg aattttaatt
      961 tataaaaagg atagtaaaaa aaatgaaaaa atagtattct atattgtctt gtttcttatt
     1021 tatattataa ttcagataag tagattattt aatgaaggaa attactttag taatataaac
     1081 tatttatttt atatattagc atttattgct tggggag
//
LOCUS       DAGSWI010000311         1115 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.316, whole genome shotgun sequence.
ACCESSION   DAGSWI010000311 DAGSWI010000000
VERSION     DAGSWI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.316"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(159..998)
                     /locus_tag="MJF80_003993"
     CDS             complement(159..998)
                     /locus_tag="MJF80_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBZ0282543.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT          460 a          165 c          101 g          389 t
ORIGIN      
        1 tataaattaa aactatccca ttaaataaaa tttgtcaagt tatactaaaa ataaaaaaag
       61 cccagaaaaa ttctagactt tttaacttaa atgtcaatat ttctttatta attcaattac
      121 atttataata ctaaatactc tcttttattt taaatacttt actttaacat ttcatgtctt
      181 atctttctaa aatacaatct tgaatttacc tcatagccaa taacgataga ccaactacta
      241 aaatacatcc aggtcataag tacaattatt tcagctatac ttccatagat tacttcatag
      301 ttagcatagt aatttgtata ataagaatat aaaaatgata ctaaaaacca agcaaaagta
      361 gcaaaaatag cacctggcgc agattcttta atagttagct ttttatttgg agtgtattta
      421 tataaaagtg taaatatcac tattattgtt attataccta cagtgtatct tagaatatcc
      481 catattttta taaatatctc atccaatcct acaagattaa aaataaaata acctattttt
      541 tccccataaa ctaaaaatat catagataaa aaaataagga ccaaaagcat tattgtaaat
      601 aaaaatgata tagataaaat tttaaaaaaa gacctagttt ctttaacttt ataggctcta
      661 ttcattcctt taataagagc ttttactgct cttgaaaatg tccacatagt aagtacaaaa
      721 ctgactgcta aaaatttcag gtttctattg tctattgcag aaactatgat tgaagataac
      781 acagcaaaag caccttctgg catcatattt ctaaacaaag ctatatactt atttaaatga
      841 agaatcggta tataagctat agaacttatt gtaaatatta aaaaaggaaa tatggacaac
      901 attaagaaga aagatacttc tgctgacttt gaacttaatt cactgtaatt ggattttgca
      961 attatatatt caatcaattt cttatctata ccaaacataa atatcccctt ttctatcaaa
     1021 ttaatttcat tatacaatat taattatact cagattcatt ctataatata attatttaag
     1081 taaaaataac acaaataaaa gctgaaaata acaat
//
LOCUS       DAGSWI010000312         1094 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.317, whole genome shotgun sequence.
ACCESSION   DAGSWI010000312 DAGSWI010000000
VERSION     DAGSWI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.317"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            238..378
                     /locus_tag="MJF80_003994"
     CDS             238..378
                     /locus_tag="MJF80_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282544.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(375..746)
                     /locus_tag="MJF80_003995"
     CDS             complement(375..746)
                     /locus_tag="MJF80_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBZ0282545.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
BASE COUNT          418 a          111 c          133 g          432 t
ORIGIN      
        1 aatagaaaaa acatacaaga ttctacaata atagattttt ttgttattat tacgagtttt
       61 ttattgcaac tgtagaataa aagtattatt atataattaa gataattatc tagggaaatt
      121 ttggataatc aaaatatatt ttataaaatc aaaatacaat tacataaact ttattaaaaa
      181 tttgttggga atttctgact aacattaaga acgtaagttt gttgggggat ggtatatgtg
      241 gaaaagtcaa acactactgt tacaaaaaaa gaaaaaattg aagatatagg aatgaaatta
      301 gaacatttat tgagtgaaga tattactttt tatgaattat tacttgagat tttgtataaa
      361 gaaaaagaag agaattaatt ctcttcttta aggaatcttt ttaaataggc attatcaatc
      421 attttttcta tagcttctct atcttctttt tcaagagaat atattttttc taagagcttt
      481 tttgtttttt cacttactga taagttctct atgattaatt tatcttcttt gcttattttt
      541 gtattatcat ttacgcctaa taaataatct gttgatgttt ctaatgcttt tgctaatcta
      601 actattattt cacttttagg ttctcttaaa ttattttcat aacgtgacaa gctagcctca
      661 gttataccga ctttagtagc aagttctttt tgatttatat ctaattcttt tcgtagattt
      721 gctattctat ctcctaaact ttccatatgt acatcactcc ttaccgaaat tataagttat
      781 tttgataaaa agtaaaataa aattaccaaa aatataaaaa aaaaaggtta aaataataaa
      841 atatattgaa aattaccatt tgtatatgta taatatgaat tatacaaatg gtaatttgat
      901 gagaggtggt atgttgaaat aagaaactga attaattaat aaatacatag aaagttttga
      961 aaacctttaa ttgagatata aacaatatgt acaaaatttt ttgcaagtaa aattaccaaa
     1021 tgtataaaat aaatcagtag catagataat tttatgcttt aatacattag attatttaat
     1081 ttagttataa tggt
//
LOCUS       DAGSWI010000313         1041 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.318, whole genome shotgun sequence.
ACCESSION   DAGSWI010000313 DAGSWI010000000
VERSION     DAGSWI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.318"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            107..859
                     /gene="cwlD"
                     /locus_tag="MJF80_003996"
     CDS             107..859
                     /gene="cwlD"
                     /locus_tag="MJF80_003996"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBZ0282546.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          463 a          108 c          158 g          312 t
ORIGIN      
        1 tgaattaaat agggacagaa atataatatc attacataat aatatattaa taccaataaa
       61 taaagatata tcaatattaa aaaaatttaa atgtttgtac taatatatgg aatattatag
      121 tgtttttata agtatacata tacagggtga tgttgtgaga aagtacataa aacatataat
      181 ttttagtttt gcaatgatat gtctagtagt agtatccata tttgaaatta aaaatatttc
      241 tgaagatgtt atcaagtata tgccagtaac aaacaaaact ataattttag atgctggtca
      301 tggaggcatt gatccaggtg cattaaataa ggataagagt acatctgaaa aggatattaa
      361 cctagcaata acacttaagc ttagagagct tatagaatca agtggtggcc ttgtaatatt
      421 aactcgagaa gatgacagta gcctttataa agaagaaaat aataaaacta caagacaaaa
      481 atataatgag aatttgaaaa atagaaaaga aataatatct aattctaatg ccaatatgtt
      541 tgtctctata catcttaatg catttgaaca atcaaaatac tatggagctc aaacttttta
      601 tcctaaagac aaacaagaca gtaaagaatt atcgaagtgt attcaagaag aacttaaaag
      661 agtagtagat aagacaaaca acagagaagt caaaccaagg gatgatatat atcttttgaa
      721 agaaaataat attccatcag tactgataga atgtggtttt ttatcaaatg aaaaagagtg
      781 taaactctta actgatgaaa catatcaaga aaaaatagca tgggcaatct acataggaat
      841 acaaaaatat ttaagttgaa ataactataa aaataggtct aaatatgtaa atagcttgta
      901 ttaaagcagt taaaatgcat taatataggc tatttttatt ttgacaaaaa aatatttaaa
      961 ataaaagtta aaaagttgtt gacttagaat aatatagatg atattatata tgagtgccca
     1021 aaaggagcac caaaataaga c
//
LOCUS       DAGSWI010000314         1039 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.319, whole genome shotgun sequence.
ACCESSION   DAGSWI010000314 DAGSWI010000000
VERSION     DAGSWI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.319"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(4..897)
                     /locus_tag="MJF80_003997"
     CDS             complement(4..897)
                     /locus_tag="MJF80_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBZ0282547.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIGIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
BASE COUNT          385 a          130 c          140 g          384 t
ORIGIN      
        1 aaattaccag tctaactcaa attcaaaatc atccgacttt atctgttcaa tttcagaatt
       61 taccttctgt ttaaatttat tagaagaaat catatataca agaaatatta aaccaaatat
      121 tatttgtaca tattgagcta ttacacataa tttaataggg tagtgtagaa gtaattgaat
      181 gttgaaaaaa tagtacatca tatgaactaa tccagctgat aaaatgcttc tacttgtatt
      241 gatatataca aaattaatta taaagtttaa aagtacaatg tttggtataa ataaaaaagc
      301 atcaaatagg gagtattgtg ccaaattata tggtatttga ttaggtgtaa aatacgaccc
      361 tagataccag attccacaaa caagccctaa aataattgaa gagcctataa atccaaatct
      421 aactaataat ttgtcaatgg catatcctct ccatccagct tcttgactaa gtatactcaa
      481 tataaaactt gctaatagca tataagctat tttagaaggt ctaaacactg ctatgtgtat
      541 ccaatctata tcagaagttt ttacattaaa gtaatttccg attattaacg aagtaataaa
      601 agttaaagca aaaaataata ttgttaatag tatccatttt attccgattt ctttaaagct
      661 taggtatcta taaataaaat cttgttttgc actttgtgga tagattgtag aaataaaaaa
      721 tagacctaca atagctggtg atgtatatgc aagataaaaa atgatttctc caagtattgt
      781 atttgtaatt ccgagcaacg caggtgtaag actgaatatc cagttcaata ctagagtaaa
      841 tgtaaagaaa tagattaagt gtttgtttgt attaaagggg gtgttatata gttgcatttt
      901 ttcactatcc ttccgtttta taatgaagtt ttaattttta ttaattgttt atttattttt
      961 atatgtatta agttatcgct tgatgttagt tattgcctat aataatatcc tattttttat
     1021 attttgatta attattttt
//
LOCUS       DAGSWI010000315         1022 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.320, whole genome shotgun sequence.
ACCESSION   DAGSWI010000315 DAGSWI010000000
VERSION     DAGSWI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.320"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            131..385
                     /locus_tag="MJF80_003998"
     CDS             131..385
                     /locus_tag="MJF80_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282548.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            430..753
                     /locus_tag="MJF80_003999"
     CDS             430..753
                     /locus_tag="MJF80_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282549.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT          425 a           80 c          163 g          354 t
ORIGIN      
        1 tttaaatata taaaactata aaggttacaa ttttggaaat agatgtaaat atatgttgta
       61 ttgtataata agagtaatat aaaatctagt aaaaataatc aaataaattt agcttatgag
      121 agggataact atggaaaata aggattttaa attaacaata tgtatatttg cttttgtaat
      181 tggagtagta gtattattct gttgtatagg tttaggtgaa tttagtgcag agcatattat
      241 tcaacaaaat ggtggaatca tggatactga ccaatataat atttatctag accaatctat
      301 ttctcaatat agaaattttg gttctatatt agctctgtta ggaggaatag ggatattgtt
      361 tgataaatat atcagaaaat actagagatt atgttttgtc taaaataaat tcttatgagg
      421 agataaaaaa tggcaacaga aatatttact gtaaaaatta cacccttaga ggcacaaaaa
      481 atgataaaag aaaatcttga tgcagattta gtttttagtg attcttataa tttaggagat
      541 gataaattta tactaattac aacttttgag aagtattatg caagaaataa tagtgatgca
      601 ggattaatag tagtttatga aaataccaca ggaaaaacag ttattaaagc tacttcaaca
      661 ggcagtgcaa ttggaatttt aaaaattgat tggggtgcag gtggaaattt acttaaaaga
      721 gtaaaaaaca tacttgctga ttatattatt taattatttt atctaactga taatatgtca
      781 agaaaatctc gtaaaagagg ttaagaacat ttttataata tatagataaa taagaatttg
      841 aaggtaaagt ttttaaggta taaagaaaaa taatataata aaacgaattg gctttataat
      901 tctttataag gcaaagagca catttcttta tataattgtt ggatattaaa taatatgtaa
      961 atacaccatt tacatataaa agtttaatta atatgttaag agaaaatgaa atggaaatta
     1021 ct
//
LOCUS       DAGSWI010000316         1019 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.321, whole genome shotgun sequence.
ACCESSION   DAGSWI010000316 DAGSWI010000000
VERSION     DAGSWI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.321"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(91..801)
                     /locus_tag="MJF80_004000"
     CDS             complement(91..801)
                     /locus_tag="MJF80_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282550.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          351 a          140 c          111 g          417 t
ORIGIN      
        1 aaaattaaat ttgaaaaaac aaaactctaa aaactaagct ttaaaaagtt aagtttcaaa
       61 aataaaattc tagagattga acttcaaaaa ttaagattct aacatatacc ttgtaaattt
      121 tcctacaacc tcttgatatt ttttacggct tataggaatt ttttcattct gaactttggc
      181 ataagtttga gttatactat ctacatggtt tagattgaca atataccttt gatgcgtacg
      241 tataaaatta gagggaagaa cagtttccat atcatttaat tttccgtagt attctaatgt
      301 tttatcaaag gtaactacag ttaattttct tagctcactt ttaaagtaga ggatatcttt
      361 tgtattaatt ttacaaagat tagttccaat ctgaactgta ataaagtttt ctttccattg
      421 ttctaatttt tttagtgctt cttttaatac tttacatatt ttttcagttt tataaggttt
      481 taagatgtag ttcaaagctc ctacatcata tccatgaaat acatagtcag aaaaacctgt
      541 gacaaagatg attatagtat cttttgaatg ttttcgtata gtttttgcta tgtcaacgcc
      601 attacttttt ccaagttcaa tatctaaaaa aataatatct atgtaataat tttcgtgttc
      661 tagattgtat actagacttt ctccatttga atattctttt atttggtagg ggtagcccca
      721 caaatccatt tctcttgtaa tgattttatt cagcatgttt ctttgttgtt tttcatcatc
      781 acaaatacaa atcgaagtca attttttatc tcctttcaaa ttttatattt atatagttaa
      841 gtatataata tattttgcta cctagatata taaaatgaaa taacaattat atccagatag
      901 caaaataaaa ttttttatat tagtatactt acacttaaaa ctattctatt aaacttattc
      961 tgaagtgcta atgattttct tattaaaaaa tctaacaatc tttcttatta taaaataca
//
LOCUS       DAGSWI010000317          966 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.322, whole genome shotgun sequence.
ACCESSION   DAGSWI010000317 DAGSWI010000000
VERSION     DAGSWI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.322"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(51..239)
                     /locus_tag="MJF80_004001"
     CDS             complement(51..239)
                     /locus_tag="MJF80_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282551.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHGYSKK"
     gene            493..669
                     /locus_tag="MJF80_004002"
     CDS             493..669
                     /locus_tag="MJF80_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282552.1"
                     /translation="MKTLMFILVIAYYITIVIKSITTIVKSITRLLDSISRLKESLHK
                     LRNRPDGNQDDFNE"
BASE COUNT          360 a          117 c          103 g          386 t
ORIGIN      
        1 taagtcaaac attattatat agagcattct tcattatgga gagtgctttt tcatttcttt
       61 gaataaccat gttgattatt tataatattt tcatttaatt tatctttatc tatcaaattt
      121 aatgcacaat taatacaaat gttagttttc atatgacttt tcttatgaaa agtaatatac
      181 ttattatcat ctttatttat tcctttatta cagtaatcac ataatatagt cttagtcata
      241 tttttattcc tttcatttta tattttccat aattaagttt aacatattta gtatatacct
      301 atgtatatat atttaaaaca atgtttttta ttgcaatata gaatatattg tataaataaa
      361 aaaatggaga atagatattt gataattgta aaattatgtt ataattaaaa tgcaagaata
      421 tatcttgcaa gaggctacaa tctaagggcg aaagttttcg gaaatcacct tcgaacgaaa
      481 gggggtgagt atatgaaaac acttatgttt atacttgtta tagcgtatta cattactatt
      541 gtaataaaat ctattacaac aatagtaaag tctataacaa gattattaga ttctataagt
      601 agattaaaag aatctttaca taaactaaga aatcgtcctg acggcaatca agacgatttc
      661 aatgaataaa tcattactta aattattttg aatttgaaac ttcgctctag gctaatagat
      721 tgtagtttct ttttttgttt ttgatacgaa tttacatctt tattatatcg cattttaaga
      781 aaaaataaaa ctatgaatat tataaatcta ttttaattaa ttttttatca tatcattatt
      841 taaaagtaga taaaactaaa aagggttctc tagaaatcaa tgtctatcta attatactta
      901 cttaccaata ccatcacttg gtgtaaacaa gactctacta cattttctcc gtactctaat
      961 ataaaa
//
LOCUS       DAGSWI010000318          963 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.323, whole genome shotgun sequence.
ACCESSION   DAGSWI010000318 DAGSWI010000000
VERSION     DAGSWI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.323"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            241..732
                     /locus_tag="MJF80_004003"
     CDS             241..732
                     /locus_tag="MJF80_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBZ0282553.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT          372 a           84 c          147 g          360 t
ORIGIN      
        1 tcttaaattt tttttaaaaa gtaatagttc ttatttatag tcaatattgc ctatagaact
       61 tctgattcat ttaattttta ggaaaaaata ataatactta cgctataaac acagaatttt
      121 agttgataat ataggtattt aaatttaaga tgaaataata cttttctcaa tatattttat
      181 tgtaactaag tttaattata agttacaatt aaaaaaacgc tacttaggag gaatgtaatt
      241 atggggatga taggatgtta tactaaaata agtgaagaga atgtttttaa attacagcaa
      301 gcagaagaaa atttacaaga ttttgttttt gaagatgcca acgaaaatag tactatcaat
      361 attgataaag catggcatgc cattcacttt actttgacag gttgtccttt tggtggagat
      421 gatgataata tatttagcaa attagtacta agtggaaaca tacttatgga aattgatgga
      481 gaatttcctg tcatgttgat tacagcaaat gatgtaaaaa aactatcaaa agcaatgaac
      541 agtttggaag aacaagcatt tagaaaaaga tttaatatta atgagatgtt agaaaataat
      601 atttatcctg ttatgaatga tgagagtgaa gatgattttt ttgaatatgt atgggcaaat
      661 ctaattgagc ttaaaaagtt tatacaagaa gcatctaatg atggactagc tgtaatattt
      721 tttataatgt gataattact aaaaattgtg aatattagtg tcatcacata tcttagatat
      781 gaagcctttt gattgtatag aagaaataca tattatatta gccttttaaa atatataagt
      841 taaatttaaa tctgtaggta aatttgtctt atgtctttat aaatatttat attagcatat
      901 ataaattagt tttaaatttt tgcaatatat agttgcagat atttttattt atcttaaaag
      961 gct
//
LOCUS       DAGSWI010000319          959 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.324, whole genome shotgun sequence.
ACCESSION   DAGSWI010000319 DAGSWI010000000
VERSION     DAGSWI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.324"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..888
                     /locus_tag="MJF80_004004"
     CDS             <1..888
                     /locus_tag="MJF80_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBZ0282554.1"
                     /translation="NKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYADLA
                     SKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDILDE
                     LEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILPSI
                     TIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIYDD
                     KNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAARLI
                     ELIQQYNLQLIDSEIQSEM"
BASE COUNT          412 a           93 c          154 g          300 t
ORIGIN      
        1 aataaaaaat tcattgttat aatactgata atatcaattt taataggtgt attagtgaag
       61 gaatattcat ctagagagat aaaaaaggat gatataaatg tcagtaagta tataaaatat
      121 gctgatttag caagtaaaaa taatgcacaa gttaactgga agtatgtagc ttccattgtt
      181 gctgtgttaa ataaaaataa cttaaaaaac gttaaggatt cacagataca agaagtatca
      241 gatttatttg ttaagaattt ttctaaaaat aataaaataa ataagttaag tgatattttg
      301 gatgagttag aatttagtaa tagacaaaaa agattggttg ataattatat agacaatctt
      361 aaggattatg gtataaaacc agaaagattg aagtctgata caaaatatat gaagtttatt
      421 gatgaaatta aaacagaagc aatacaaaat tataaggatt ataaaatact tccatctata
      481 actattgcac aagctataat agaatctagc tggggcaagt caactttggc aaaacaatat
      541 aacaatctat ttggtataaa ggctgacgct tattggaaag gcaagtcagt gactttagaa
      601 acgaaagaac atttagatac aattatagat gataaattta gaatatatga tgataaaaat
      661 gaatctataa aagaccatgc aaaattttta gccacaaata aaaggtataa aaacaatggt
      721 gtatttgatg caaaaacgta tatatatcaa gctaaagcat tagaaaaggc aggttatagt
      781 actgcgaagg atgaaaatgg caatagtata tatgcagctc gtctaataga attaattcaa
      841 cagtataatc ttcaattaat agacagtgaa attcaaagtg aaatgtaact ttgattaaat
      901 ttttattgat aaggctatga tttttaaaat catagcctta ttttgacatg cttttactt
//
LOCUS       DAGSWI010000320          893 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.325, whole genome shotgun sequence.
ACCESSION   DAGSWI010000320 DAGSWI010000000
VERSION     DAGSWI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.325"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            130..642
                     /locus_tag="MJF80_004005"
     CDS             130..642
                     /locus_tag="MJF80_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBZ0282555.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
BASE COUNT          383 a           99 c          122 g          289 t
ORIGIN      
        1 tttagttctg aatgttactt ttacttagat aaaaccaaca caaagtttat tctttatata
       61 agtaatagct aaaatttaaa gtaatgatat tatataaata gaaataattt tatttaaagg
      121 agaatctgta tgttagaagt tataaaaaat agacgttcaa taagaacata tatagataaa
      181 aatatagaag aagataaaat aactgaaata ctaaaatcag ctatgcaagc accttcatca
      241 aaaaatgctc aaccttggga atttattata gtagatgata aggaactgct gaaacagctc
      301 tcaaagtcac agcatagagc aaagcatata gaatttgcac cattatgtat agtagtgctt
      361 ggaaatagag ataaattctt aaaacctggt aaatggatac aagatttagg tgcttgcaca
      421 caaaatttat tattagaagt tactaatcaa ggtctagctg cttgttgggc aggagtattt
      481 cctaaaaata aagtggtaaa taaagttaga caaactttag acttaccatt aaaattagtt
      541 ccttatgcac ttatatctat tggatatagt gaagaaaaaa atgagtttat agaccgattt
      601 gatgaaaata aaatacatag aaatgtatat aaaaatagat aatgtttagt aatataaatg
      661 aaaatacata gtaaaaatgc ctttagaata catttgtatt ctaaaggcat taactttgat
      721 taaacaccta attaacctct aaggtaaatt aataatgata atcacaactt taagaaaaat
      781 atagccctta atgattatca taaaaattaa ttttttgaaa aaactcatat tgactcttga
      841 agaagtacat acaaataaat tttgctttat gaatattcaa ataatttatt tta
//
LOCUS       DAGSWI010000321          759 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.326, whole genome shotgun sequence.
ACCESSION   DAGSWI010000321 DAGSWI010000000
VERSION     DAGSWI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.326"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            175..558
                     /locus_tag="MJF80_004006"
                     /pseudo
     CDS             175..558
                     /locus_tag="MJF80_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          345 a           63 c          120 g          231 t
ORIGIN      
        1 gtttgaaagt taaataaatt tatagatatg aacattattt tacacttaaa aacagactaa
       61 acacgtagag tacctggtaa aaattaaata tagtttttgt tctaaaaagc tcctattata
      121 atttaagcta gataaatttg aagaagtgac ataaattgaa agaaggaata tattatgcta
      181 aataaaaaat taccagatac agaattaaaa attatgaaat gcatatggca tactggttat
      241 aaaactgtaa tatcaaaaga tgttgcagat gaaatagaaa aaacatatgg ttggaagcat
      301 acaactacaa ttatactttt agctagatta accaaaaaag gttttttaat atcatagaga
      361 attgggaggc atgtacacta tacagtatta ataaaagaga gagaatatct caaaatagag
      421 ggaaaaagag tttttagagg tttacataac aatcctttat cagaattaat ttcaaaatta
      481 catgatggag aagaaataac tgaagaaaaa ataatagaaa taggaagttg gataaaaagc
      541 tggaaatata aagattaata aataaaaggt agctaataat ataagtgcta ccttttttat
      601 gtggataata ttggtgataa gttgtggaaa acttatataa aaattgtgga taaagtgttt
      661 tgctgacatt ttactgacaa aagttaaaaa aataaatgtt atatattgta aaatgttgaa
      721 aaatagccat atatataaaa tataagtaac actatagat
//
LOCUS       DAGSWI010000322          745 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.327, whole genome shotgun sequence.
ACCESSION   DAGSWI010000322 DAGSWI010000000
VERSION     DAGSWI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.327"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            139..>745
                     /locus_tag="MJF80_004007"
     CDS             139..>745
                     /locus_tag="MJF80_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBZ0282556.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLS"
BASE COUNT          309 a           85 c           91 g          260 t
ORIGIN      
        1 ttgttaatat atatttatca ttatattata taataatata gatagttact atatcttata
       61 attagataaa tcataattat atattattgt taaaactcaa ttatataatt tttaataata
      121 ttattttagg aggtaaaaat gataaacgat attaaaaata aattcaaaaa ttacaaacca
      181 tatataaatg gatacaagaa tatgaagaga gcatctgttt taattccaat agtagaaata
      241 aataacacac actatatatt atttgaagtt cgctcaaaaa atttgaagca tcaacctagc
      301 gaaatttctt ttccaggtgg taaaatagaa tcaggtgaga ctccatatga agctgttatt
      361 agagaaactt gtgaggaact tggaactttt agtgacaata tagaagtaat atcaagtctt
      421 gacctcctta taacaccagt aaagtttatc atacatccat acttaggata tataaaaaat
      481 attaataatt taaatataaa caaggatgaa gttgaccata cttttttggt tcctataaaa
      541 tatcttttag agaatcctcc aaatacttat agtaatactg taaaagtttt tccagatgaa
      601 aatctaccat atggttcaat tccaaaccaa aaagattata aatttgaaag tggaagttat
      661 cctattctgt tttataaata taaagattat gtaatttggg gaatgacagc aaaaatacta
      721 gaaaactttt taaatttttt atcag
//
LOCUS       DAGSWI010000323          743 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.328, whole genome shotgun sequence.
ACCESSION   DAGSWI010000323 DAGSWI010000000
VERSION     DAGSWI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.328"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            7..705
                     /locus_tag="MJF80_004008"
     CDS             7..705
                     /locus_tag="MJF80_004008"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBZ0282557.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
BASE COUNT          301 a           90 c           96 g          256 t
ORIGIN      
        1 atttttatga atgaaaaatt aattggtatt ttggctggaa tgggtccaag gtcaacagcc
       61 ccttttgttg atttaattat tgatgagtgt caatctcaat atggagcaaa atatgatgat
      121 gaatttccaa agatgatgat atattcatta cctaccccat tttatattga ccgtccaata
      181 aatcatgacc ttatgaaaga aactataatt gatggattac aaaagcttga atcaactggt
      241 gtaaatttta ttgcaatgcc atgcaattca gcacatatat actttaaaga attaaaagag
      301 tctattaata taccaatatt aaatattgta gaagaaacag ttaaaaaatt acctactatc
      361 tcacaaaaag taactctttt ttcgactagt tcaacttttg agtctacaat ttaccagaat
      421 ggtataatac ataatggaca tgaatttata tttaaagatg aatggcaaat aaaattaaac
      481 aatcttattc aaaatataaa aatggataaa gaaaatccac acaatattga tatttggaat
      541 aaacttattg aagatgttaa aaatgaatct atagaaaata tagtaattgc ttgtactgat
      601 ttaaatgtag ttttagaaaa atcatatcct tcaataaata ttgttgattc atcaaaatgc
      661 ttggctgaat cagttgtcaa taaatatcta aaattagtta aataaatttt tcatacacta
      721 tattataaaa aatatatgtt ata
//
LOCUS       DAGSWI010000324          699 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.329, whole genome shotgun sequence.
ACCESSION   DAGSWI010000324 DAGSWI010000000
VERSION     DAGSWI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.329"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(198..677)
                     /locus_tag="MJF80_004009"
     CDS             complement(198..677)
                     /locus_tag="MJF80_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBZ0282558.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          275 a          108 c           54 g          262 t
ORIGIN      
        1 cttttataaa aaaatgtgct tttaatatct actaataaag ttctttattt taattaaaac
       61 ataattatat tttagtatta taatctttta attttagcat tatagtttat gtattatacc
      121 ctaatatatt ttattttaaa ttactagtta gtcactcttt tcgggttatt tatctcctat
      181 atattaaagt gtcacatcta aatagttaaa tcattatcct cttttatctt tattgccttt
      241 acaaatccat ctgcaattac aaaactcata agagaagtta taaaatatac aaatactgga
      301 acagtaaaat aaaatccaaa tcctaaatct agaaatctca ttccttttcc agtagtacca
      361 aaagttgaaa taaactcaat tagagagttt aaaagcaaaa gatatccaat aatcctaaat
      421 ctttttacat tctcttttac aaataggcta tattcagaac tacctaatac ttctatcaat
      481 gattttataa tgataaaata tgatacacct aaaataaaca ttaatattcc ataaatagta
      541 ccttgtagag ttgcttctcc actaaaacag aggtacataa aacttttaaa tcctcctgca
      601 acaattacta atcctaaaag aaaaaataaa atttgtaata taccacttag taaatttttt
      661 ataagtttat tagacatcat gccaactcct ttaaaatat
//
LOCUS       DAGSWI010000325          685 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.330, whole genome shotgun sequence.
ACCESSION   DAGSWI010000325 DAGSWI010000000
VERSION     DAGSWI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.330"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     regulatory      <1..68
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            151..636
                     /locus_tag="MJF80_004010"
     CDS             151..636
                     /locus_tag="MJF80_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBZ0282559.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          304 a           59 c          113 g          209 t
ORIGIN      
        1 tctaatgacg caaaactata gggactaaaa tctttatgta ataaaggtca tgtcagccag
       61 ttgccaaaaa gataataatt ctttttgtta aaagtatttt tagagttata tattttaata
      121 acttaatatt tggcaagagg agggcttaag atgatatctg aattaatgtt aaaaaatttt
      181 cgcaaatcta gcatcaattt taagactaaa attaaattga atttaagaga aaaaccagac
      241 ataaatagtt tgaaattaaa aagtatacca gaaggtaaaa ttgttaagtt aaagtgtgta
      301 gatggaatat gggcagaagt tgagagtaat tatgacaaag gttggttact ttataaatat
      361 ttagaaagat taagtaatgc taaaaacaat agtaatgata caataaaaaa tagaaaaaag
      421 aattacatag gaaatataag aacaaatgga ttaagtttag aacttagaaa tgatagaaca
      481 ttagaatcta aagttataac tacaatccct gatggtttta aagttgaagt atgttatagc
      541 gttggaaaat gggcaagagt taatatcaat aaaaatgaaa aacgatattc tggatatgta
      601 tataatcaat acatagaaga gcatttaagt ttataatacg atactagcaa gctttaaata
      661 tattaataaa tcgcaaaaaa gaatc
//
LOCUS       DAGSWI010000326          664 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.331, whole genome shotgun sequence.
ACCESSION   DAGSWI010000326 DAGSWI010000000
VERSION     DAGSWI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.331"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>664)
                     /locus_tag="MJF80_004011"
     rRNA            complement(<1..>664)
                     /locus_tag="MJF80_004011"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          152 a          191 c          151 g          170 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 accacctgtc accaatgtcc ccgaagggaa ctctccgatt aaggagatgt cattgggatg
      541 tcaagcttag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc cgctacttgt
      601 gcgggtcccc gtcaattcct ttgagtttca ctcttgcgag cgtacttccc aggcggagta
      661 ctta
//
LOCUS       DAGSWI010000327          659 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.332, whole genome shotgun sequence.
ACCESSION   DAGSWI010000327 DAGSWI010000000
VERSION     DAGSWI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.332"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..>659
                     /locus_tag="MJF80_004012"
     rRNA            <1..>659
                     /locus_tag="MJF80_004012"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          187 a          136 c          195 g          141 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaagg
//
LOCUS       DAGSWI010000328          634 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.333, whole genome shotgun sequence.
ACCESSION   DAGSWI010000328 DAGSWI010000000
VERSION     DAGSWI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.333"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            34..>634
                     /locus_tag="MJF80_004013"
     CDS             34..>634
                     /locus_tag="MJF80_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBZ0282560.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSN"
BASE COUNT          223 a           55 c           84 g          272 t
ORIGIN      
        1 aaatataatt taatcatttt tgaggatatt attatgttag atgcatttat aacaattgtt
       61 tcttctatta tagaaatatg gtcatgtaaa aaagtttttg attatactag tcagtcaaag
      121 accgacttaa aaaaactaaa tatgtatttt ctcttttctg tttttctcat tatttcttct
      181 ttatatataa atataggagc taatagtaga atcttgatat gtatatttgt aatgttcgtt
      241 ttctataagt ctaactataa ggttaatata tataaatgtc tggtaatctc atttttttat
      301 tggttaatat taataggtat aaatggatta agtatgttat tagtagcaac agtaagcaat
      361 ttggataata tgaatgtttt acttagtggc agtttatata gatatgaagc aatattcttg
      421 ggaaagataa tgttaggtat attactttat gtttataagg cttcttttaa aaattttaaa
      481 ataagtaaaa aagagatata catatgtatt cctatgatag caaatttatt gtcatttttt
      541 gtactttatg attatattat taatcttgta aaaatctctc ctgaaagaag aactgctttt
      601 ttagtaatag ttttactatt aatattatct aaca
//
LOCUS       DAGSWI010000329          634 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.334, whole genome shotgun sequence.
ACCESSION   DAGSWI010000329 DAGSWI010000000
VERSION     DAGSWI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.334"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..>634
                     /locus_tag="MJF80_004014"
     rRNA            <1..>634
                     /locus_tag="MJF80_004014"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          178 a          138 c          183 g          135 t
ORIGIN      
        1 tgatggggag cgaaatttag tagcgaagta gctgatttca cactgtcaag aaaagtctct
       61 atcgaggtta aaggtacccg taccgcaaac cgacacaggt gggtgaggag agtatcctaa
      121 ggccagcgag agaactgttg ttaaggaact cggcaaaatg accccgtaac ttagggataa
      181 ggggtgccac catcaggtgg ccgcagagaa taggcccaag cgactgttta ccaaaaacat
      241 aggtttctgc taagtcgcaa gacgatgtat aggagctgac gcctgcccgg tgctggaagg
      301 ttaaggggat ctgttagagc aatcgaagca gtgaacttaa gccccagtaa acggcggccg
      361 taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cccgcacgaa
      421 aggcgtaacg atttgggcac tgtctcaaca acagactcgg tgaaattgta attccggtga
      481 agatgccgga tacctgcgac aggacggaaa gaccccatgg agctttactg tagcttgaca
      541 ttgggtcttg gtactacatg tacaggatag gtgggaggct ttgaaaccag gacgccagtt
      601 ttggcggagc catccttggg ataccaccct tgta
//
LOCUS       DAGSWI010000330          629 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.335, whole genome shotgun sequence.
ACCESSION   DAGSWI010000330 DAGSWI010000000
VERSION     DAGSWI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.335"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     regulatory      complement(257..347)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          235 a           73 c           55 g          266 t
ORIGIN      
        1 tattttttca atgaagtccc atgaaaatat aaaaaaattt taatatgtat ttaaatattt
       61 cttcgaaaag ttgctctttt ttgtaaaata gtaacttttt aagataattt ttctttattt
      121 tatgttataa tttggtaaag tatgttaatt ataggtattt tctactgaaa taaagtgatt
      181 ttttcataac aaaatgactg ccaaaaaggc agtcattcta gacttttctt aggtatacaa
      241 tacacctagg actctttttg gcaactggct gacatagttc atgtatataa accttagtcc
      301 ctatagcttt gcgtcactag atttctctag atttgcctat ttagttttaa cttaagtata
      361 caataaataa caaatttttt aaatatcttt tttgtttata atcaaatatt ttatatttta
      421 tatttagtta ccaataatta caaatattat aaacactata ctctagaatt ttataataaa
      481 ctctttgaaa atatttttaa tttataaaaa tataagacaa atctcatctt aattctatat
      541 ttttccatat atttcaattt tatttgtaca atatatcaat atttaaaaat gtcaattaac
      601 gaaacttaaa taaatgatat ataaaaaat
//
LOCUS       DAGSWI010000331          591 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.336, whole genome shotgun sequence.
ACCESSION   DAGSWI010000331 DAGSWI010000000
VERSION     DAGSWI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.336"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            359..>591
                     /locus_tag="MJF80_004015"
     CDS             359..>591
                     /locus_tag="MJF80_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBZ0282561.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPII"
BASE COUNT          253 a           35 c           86 g          217 t
ORIGIN      
        1 atttttataa tagaaatttt aatttatatc tatattaaat tgttaaagat ataattatga
       61 gaataaaaaa tatagctttg aattatataa ttgtatatgc ttatctttat acagtaaata
      121 aaaaatcaaa tgaaatacca aataaagtgt atatcttaaa ataacttact agagatacaa
      181 ggatgctatg tgaattttga agtgtgcagt ttagataata aatatatata aataataaag
      241 aaaatggatt tatagtattt aaaatttctt ataagggtaa aaaatgctat tagaaaatgt
      301 actaaaaata tttttccaat tctttataga aaaataatga tatttaggag agattgtaat
      361 gcaaattgat gtattgataa ttgatggaga taagaatagt tctcaagagc tgaaaaattt
      421 tttagaagga aaggggataa atgtctattt agcatataat tgtaaagatg ctattataaa
      481 aatatttagc aagaaatttg acttaatatt tttagagata attttagttg atggtgatgg
      541 ttgggatttt tgtaaaaaaa ttagaaatat aactacttgc ccaattattc a
//
LOCUS       DAGSWI010000332          571 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.337, whole genome shotgun sequence.
ACCESSION   DAGSWI010000332 DAGSWI010000000
VERSION     DAGSWI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.337"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
BASE COUNT          246 a           46 c           71 g          208 t
ORIGIN      
        1 tcttttttat attgctgtta attcaaaatg gagttttaaa aacaggttat caaaatagag
       61 ctacatttta tcaagataat tattttaata gagaggataa aaatgataag ataaaattta
      121 tgactataaa gatttttata tttttgtcat taccattatg ttttaatata ctattcaatt
      181 atttaacctc ttaaattata tttagatata ggagtcttat gataaaagta agcttatgtg
      241 ttatttgtag taaatataaa gtgcatatag tgataaaaca aaagttttat ataaatgaag
      301 aaaaaaatat aaaataattt atttaactac ttaaaaataa actaaaaggg ataataaaat
      361 agcttttagg ttatggtaat aaaggtattt aaaaagagtg cataagcact ccaattgtga
      421 gaaatacata cacaaatcat tattacaaca atgaaaaatg tacatataag agacgttttg
      481 tatttgtttt aagtgataat attttaatac taactaaata attatcacaa tagtaaaaaa
      541 gaaaaaggag tattttgtac tccatttgtc a
//
LOCUS       DAGSWI010000333          531 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.338, whole genome shotgun sequence.
ACCESSION   DAGSWI010000333 DAGSWI010000000
VERSION     DAGSWI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.338"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..>531
                     /locus_tag="MJF80_004016"
     rRNA            <1..>531
                     /locus_tag="MJF80_004016"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          144 a          112 c          175 g          100 t
ORIGIN      
        1 accaaggcga cgatcagtag ccgacctgag agggtgatcg gccacattgg aactgagaca
       61 cggtccaaac tcctacggga ggcagcagtg gggaatattg cacaatgggc gaaagcctga
      121 tgcagcaacg ccgcgtgagt gatgaaggcc ttcgggtcgt aaaactctgt cctcaaggaa
      181 gataatgacg gtacttgagg aggaagcccc ggctaactac gtgccagcag ccgcggtaat
      241 acgtaggggg ctagcgttat ccggatttac tgggcgtaaa gggtgcgtag gcggtctttc
      301 aagtcaggag tgaaaggcta cggctcaacc gtagtaagct cttgaaactg ggagacttga
      361 gtgcaggaga ggagagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga
      421 acaccagttg cgaaggcggc tctctggact gtaactgacg ctgaggcacg aaagcgtggg
      481 gagcaaacag gattagatac cctggtagtc cacgctgtaa acgatgagta c
//
LOCUS       DAGSWI010000334          522 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.339, whole genome shotgun sequence.
ACCESSION   DAGSWI010000334 DAGSWI010000000
VERSION     DAGSWI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.339"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
BASE COUNT          209 a           74 c           57 g          182 t
ORIGIN      
        1 tagctaccat cagcagacat atctggaaaa tctaagattt tgtaagttat ataaaccccc
       61 agtgccataa tcgataaaat taaactttgc gtcattacag atataatacc tgacataaat
      121 aaaacactcc tttgaaaata tttttataat tttaattatt tgctaatttt gtgtataaaa
      181 aaaccacata gtgtacaaaa ctatgtggta aaaacacgaa taagccacat agtttaatct
      241 atgtggctct tatatagtta aatgaaaact ttataaacaa tatagccaac atagatacaa
      301 tcccaaaatt ttagtagtct taaggggtct gtatctatgt taaaacgtag gtacaaaccc
      361 taaaaatagt ggctataata ataataatat tttctattgt ttaataaagt attcataatt
      421 atctctccct tttaatattt taattagtca gaaaattata tgtattctcg acttgttata
      481 aattataaat tatacaataa taaaagtcaa ctgctaaatt ta
//
LOCUS       DAGSWI010000335          496 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.340, whole genome shotgun sequence.
ACCESSION   DAGSWI010000335 DAGSWI010000000
VERSION     DAGSWI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.340"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            190..384
                     /locus_tag="MJF80_004017"
     CDS             190..384
                     /locus_tag="MJF80_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282562.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT          194 a           26 c           78 g          198 t
ORIGIN      
        1 tagatattta atttataaat taccaacaaa cgttagaaat tgatattagt ttttgttgat
       61 tgagacttta tatttagaaa atatgattga aagtaattta ttatgaaaat atttagaata
      121 atttatgaga aggtaaaaag aataatttat gagaatacta tatataaaaa attattgggg
      181 gaatgtctga tgtttcaagt aattaatttt ttatgtttat tttgtgttgg atttttggtt
      241 cttgatgtta tgatatattt ttgtaaggat gcaatggata tttttgaggg tacaactaat
      301 gttaataaag ttaagagtat aaatgtttgg gcagatagag tgagagctaa ttcatattct
      361 tatgaaaata aaattgcaaa ataggtaaaa ttatatatgc atgcattgtt tgtaatatca
      421 agggttatat ctattagttt taattatatt tattaaaaaa aaccaaaagt tataaccttt
      481 tttaaaataa aaaaca
//
LOCUS       DAGSWI010000336          495 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.341, whole genome shotgun sequence.
ACCESSION   DAGSWI010000336 DAGSWI010000000
VERSION     DAGSWI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.341"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
BASE COUNT          213 a           55 c           40 g          187 t
ORIGIN      
        1 aaactgtaga tagtaaattt aacctgagaa taattagcta tacctttata agaaaattta
       61 atataactct acaattaact aatgctaaaa acaaaatctt aataaatata tgcttaaaac
      121 cttaaatgtg aactatctat ttttaataat atagatagtt taactttcta acacaccatt
      181 aattagcaat ttattaatat aaaaatatat taaacttcaa tttttattta tattaattac
      241 tttttactcc tacaatttgg cataaatact attattaaac atacaaaact tctagaaata
      301 gataataaaa tgacattacc ttgtatattt ttaatctata ttttagaaaa ggagactact
      361 atgtttaaag aaaatttatt ggaatcatca gaaaaaattg aaattgaaat tttaaaaaag
      421 ctttatatta ataatggtat attttcaaag tataaacttt gtgatgaatt gcacatttct
      481 tttccaacgt taaaa
//
LOCUS       DAGSWI010000337          494 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.342, whole genome shotgun sequence.
ACCESSION   DAGSWI010000337 DAGSWI010000000
VERSION     DAGSWI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.342"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..250
                     /locus_tag="MJF80_004018"
     CDS             <1..250
                     /locus_tag="MJF80_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBZ0282563.1"
                     /translation="LENKMIKLTPTENRLLNYFIENAEKTLTIKEIYEHVWMNEYLDD
                     NYSVVVAVSGLRKKIEKDYKNPQKIITIREVGYYFNKI"
BASE COUNT          229 a           31 c           75 g          159 t
ORIGIN      
        1 gttagaaaat aaaatgataa agttaactcc aacagaaaat agattattaa attactttat
       61 agagaatgct gaaaagactc taactattaa ggagatatat gaacatgtat ggatgaatga
      121 gtatttagat gataattata gtgtagtggt tgctgtgagt ggattaagaa agaaaataga
      181 gaaagattat aaaaatcctc aaaaaattat aacgataaga gaagtagggt attactttaa
      241 caagatataa agctttataa acagaatgaa tgataaggca tagataatat gttagtatat
      301 tataagagtt tttgttctaa cgatatattt gtaaaattag atttaattta cttaattaaa
      361 ttatagcagt agaataaata taaaaaatgt ctgaaatatt atagacatca gtaaaatata
      421 taaaactata atataagtcg taataataaa acaaatgaat attaattaat aaaaagatag
      481 tagctatttt tata
//
LOCUS       DAGSWI010000338          481 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.343, whole genome shotgun sequence.
ACCESSION   DAGSWI010000338 DAGSWI010000000
VERSION     DAGSWI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.343"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..>481
                     /locus_tag="MJF80_004019"
     rRNA            <1..>481
                     /locus_tag="MJF80_004019"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          115 a          111 c          151 g          104 t
ORIGIN      
        1 actgtcaggt gggcagtttg actggggcgg tcgcctccca aaaggtaacg gaggcgctca
       61 aaggttctct cagtacggtc ggaaatcgta cgtagagtgt aaaggcaaaa gagagcttga
      121 ttgcaagaca tacaggtcga gcaaggatga aaatcggact tagtgatccg gtggttctgc
      181 gtggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
      241 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcatca catcctgggg
      301 ctgtagtagg tcccaagggt tgggctgttc gcccattaaa gtggtacgcg agctgggttc
      361 agaacgtcgt gagacagttc ggtccctatc cgtcgcaggc gtaggaaatt tgagaagacc
      421 tgtccttagt acgagaggac cgggatggac gtacctctgg tgtaccagtt gtcctgccaa
      481 g
//
LOCUS       DAGSWI010000339          436 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.345, whole genome shotgun sequence.
ACCESSION   DAGSWI010000339 DAGSWI010000000
VERSION     DAGSWI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.345"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            197..313
                     /gene="rrf"
                     /locus_tag="MJF80_004020"
     rRNA            197..313
                     /gene="rrf"
                     /locus_tag="MJF80_004020"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            319..407
                     /locus_tag="MJF80_004021"
     tRNA            319..407
                     /locus_tag="MJF80_004021"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:353..355,aa:Leu,seq:taa)
BASE COUNT          142 a           73 c           93 g          128 t
ORIGIN      
        1 tttgatgtca ttcattaaga tatatgtgca gtttttagag tgctaactct atatttttaa
       61 acttgtcaaa acttatggat ttttaatagt tttataaggc aaaaaatcga ataaaaaaag
      121 tattgacaca ataagacaaa gatgttatta taatacttgt cttagaaaaa aagttaaatg
      181 aaaatttaga caataatctg gttataatag cagagaggat acacctgttc ccattccgaa
      241 cacagaagtt aagctctcta gcgctgatgg tacttggtgg gaaactgcct gggagagtag
      301 gacatagcca ggtgatgtgc cgaagtggcg gaactggcag acgcacagga cttaaaatcc
      361 tgcgggactt acctctcgta ccggttcgat tccggtcttc ggcaccacaa attattgtgg
      421 gccattagct cagttg
//
LOCUS       DAGSWI010000340          429 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.346, whole genome shotgun sequence.
ACCESSION   DAGSWI010000340 DAGSWI010000000
VERSION     DAGSWI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.346"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..306
                     /locus_tag="MJF80_004022"
     CDS             <1..306
                     /locus_tag="MJF80_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282564.1"
                     /translation="NMGIGTLVLGIASIIIGITLFKKFTFIKDTTAIIVGSFIYQFTI
                     YFAMSLGMLSTDLKLITAIVIIAFLATGNLNISLKKNKCKASTKNKSEKRGVVDVTN"
     gene            293..>429
                     /locus_tag="MJF80_004023"
     CDS             293..>429
                     /locus_tag="MJF80_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282565.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KS"
BASE COUNT          163 a           59 c           62 g          145 t
ORIGIN      
        1 aatatgggta taggaacttt agttcttgga attgcctcta tcataattgg tattacacta
       61 tttaagaaat ttacttttat caaagatact actgcaataa ttgttggctc ttttatatac
      121 caattcacta tatactttgc aatgagttta ggaatgttat caactgactt aaaactaata
      181 actgccatag ttataatagc tttcctagct actggtaact taaatatttc actaaaaaaa
      241 aacaaatgca aagctagtac caaaaataaa tcagaaaaaa gaggtgttgt agatgttaca
      301 aattaaaaat ctttcaaaga gtttccctaa tccatatggt gaacctaata ctatctttga
      361 aaatttatca atcgatatag aagatggtga atttgtaagt atcataggta gtaatggaac
      421 tggtaaatc
//
LOCUS       DAGSWI010000341          407 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.347, whole genome shotgun sequence.
ACCESSION   DAGSWI010000341 DAGSWI010000000
VERSION     DAGSWI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.347"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            211..327
                     /gene="rrf"
                     /locus_tag="MJF80_004024"
     rRNA            211..327
                     /gene="rrf"
                     /locus_tag="MJF80_004024"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            334..405
                     /locus_tag="MJF80_004025"
     tRNA            334..405
                     /locus_tag="MJF80_004025"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:366..368,aa:Asn,seq:gtt)
BASE COUNT          130 a           65 c           88 g          124 t
ORIGIN      
        1 gatactaata ggtcgaggac ttgaccaaga tttatttgat gttattcatt aagatatatg
       61 tgtagttttt agagtgttaa ctctatgtaa agtactaact tcaattgatg aagtatatat
      121 cgtaaaaaca attactattt agtacttcaa aactattgac atttaaaaat aaagatgtta
      181 atataatact tgtcttataa ataggcaata tgtggttata atagcaaaga ggatacacct
      241 gttcccattc cgaacacaga agttaagctc tttagcgctg atggtacttg gtgggaaact
      301 gcctgggaga gtaggacgta gccacgtgaa tattccaagg tagctcaatg gtggagcaac
      361 cggctgttaa ccggtaggct gtgggttcga gccccaccct tggagcc
//
LOCUS       DAGSWI010000342          384 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.349, whole genome shotgun sequence.
ACCESSION   DAGSWI010000342 DAGSWI010000000
VERSION     DAGSWI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.349"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>384)
                     /locus_tag="MJF80_004026"
     CDS             complement(<1..>384)
                     /locus_tag="MJF80_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282566.1"
                     /translation="ILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYD
                     LKNEYEIIIDTKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNY
                     SSWKLSESFKGKFIEAKTVRLDDIKN"
BASE COUNT          122 a           60 c           33 g          169 t
ORIGIN      
        1 gttttttata tcatctaacc tcacagtttt tgcttctata aacttaccct taaatgattc
       61 tgataatttc caggaactat aattcttatc tttttgaata caaggatata ataatataca
      121 tctttttgca ttttcatatg ctgttatata tgcatacatc tggtatatat cacttgacct
      181 ataaaaaaca tttgaatcta cttcaatagc tttccattta gtgtcaatta taatttcata
      241 ttcatttttt aagtcatata aaactatatc cggtcttaaa ttaaagtttt tcttaccagt
      301 ttgttcattt ttcaaaagaa atttactctt atcttgtata tatgtttctc taaatgaatt
      361 gtcccatata cttttgatta aaat
//
LOCUS       DAGSWI010000343          363 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.351, whole genome shotgun sequence.
ACCESSION   DAGSWI010000343 DAGSWI010000000
VERSION     DAGSWI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.351"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            103..267
                     /locus_tag="MJF80_004027"
     CDS             103..267
                     /locus_tag="MJF80_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282567.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT          149 a           23 c           63 g          128 t
ORIGIN      
        1 tagtttaaaa aaataaaggg actaatttgt ctaacgttta ttggtgaaaa ataagtctat
       61 agaagttata ataaatatat aaaaatgtat gagaggtgtt atatgtttgg taaaccaaaa
      121 gaaagtgtta gatttttaga ctttaatgat gtagcagaaa agtttggaga gaaaaaggct
      181 gttgcaattt ttgatgccat agctggaaaa aaatctaaag aacatattat caaagatata
      241 gaaaaattag taaaaaatga agtttagata tttataaggt atattgagct tgagggtagc
      301 tctttatacc ttctttttta tgatttttag ataatatttt aaaatttaaa taagaaactt
      361 att
//
LOCUS       DAGSWI010000344          348 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.352, whole genome shotgun sequence.
ACCESSION   DAGSWI010000344 DAGSWI010000000
VERSION     DAGSWI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.352"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            92..319
                     /locus_tag="MJF80_004028"
     CDS             92..319
                     /locus_tag="MJF80_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBZ0282568.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
BASE COUNT          146 a           32 c           56 g          114 t
ORIGIN      
        1 accataataa ttcctttata atttgtttga ctatgtcaaa aattaccaat ataatgaaat
       61 taatgagttt atacaaattg gaggaaataa tatgaatagt ctgttaaaag aagcaataga
      121 tgaattaaaa aacatccatt gcggggaagt ttttttatta aaagaattgt tcaaaggcta
      181 tgaatggaac aggttggaga tgaatatcag attgaaatta ggaaaacttt ttttgtatga
      241 agtggtaact aatcccaatt taaatgtaga ggtcatcaat aaaaattctg cttatcagca
      301 aatgtataaa aaaaaatagt gacttaaatg tatataatag tataatct
//
LOCUS       DAGSWI010000345          344 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.353, whole genome shotgun sequence.
ACCESSION   DAGSWI010000345 DAGSWI010000000
VERSION     DAGSWI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.353"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..>344
                     /locus_tag="MJF80_004029"
     CDS             <1..>344
                     /locus_tag="MJF80_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282569.1"
                     /translation="AFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGI
                     LVMGMLYSINLRIMGKSNIPLFSFKHLFNGEISPIVLALAFVFICKVLLDLFLKTGLG
                     YTLKGVGDNSQM"
BASE COUNT           97 a           53 c           55 g          139 t
ORIGIN      
        1 gcattttctc ttactaatgg tatttctcct gtagtagcaa ctttaatggc tatcttatgt
       61 ggttgtactg ctggtcttgt tacagggatt cttcacatta agtttaagat atctaactta
      121 ctttctggta ttttagttat gggaatgcta tactctatca atttaagaat aatgggaaaa
      181 tcaaatattc ctctatttag ctttaaacac ttattcaatg gggaaatatc tcctatagtt
      241 ttagccttag cttttgtatt catctgtaaa gtccttttag atttattcct aaaaacaggt
      301 cttggataca ctttaaaagg tgttggtgat aactctcaaa tgat
//
LOCUS       DAGSWI010000346          326 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.denovo.354, whole genome shotgun sequence.
ACCESSION   DAGSWI010000346 DAGSWI010000000
VERSION     DAGSWI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.denovo.354"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            88..>326
                     /locus_tag="MJF80_004030"
     CDS             88..>326
                     /locus_tag="MJF80_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBZ0282570.1"
                     /translation="MNLKLIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLN"
BASE COUNT          134 a           30 c           32 g          130 t
ORIGIN      
        1 aataatattg actgtcatga atttataata ttcaataaaa tattttaatt tagttaagaa
       61 tctatattgt aaaggagtat taaattaatg aacttaaaat taatatggat aaatattcta
      121 aacccagtat ttattaaaaa tgaaataaaa agaaaaaaaa tacaaattat tatctttttc
      181 tttataaact ttggtttaac ttattcatta ggtttactgt tatattataa taaatttatt
      241 gatccagaaa actttgcatc attcatgatg atattacctc tatcttcagt tgctattgca
      301 aagttttata ctgaaggatt aaataa
//
LOCUS       DAGSWI010000347        12153 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.guided.125, whole genome shotgun sequence.
ACCESSION   DAGSWI010000347 DAGSWI010000000
VERSION     DAGSWI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.guided.125"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            432..1838
                     /locus_tag="MJF80_004031"
     CDS             432..1838
                     /locus_tag="MJF80_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBZ0282571.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1866..2714
                     /locus_tag="MJF80_004032"
     CDS             1866..2714
                     /locus_tag="MJF80_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBZ0282572.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLSEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3375..4067
                     /locus_tag="MJF80_004033"
     CDS             3375..4067
                     /locus_tag="MJF80_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282573.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            4064..4999
                     /locus_tag="MJF80_004034"
     CDS             4064..4999
                     /locus_tag="MJF80_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0282574.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYMDAVHSGIVIGQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            5147..6070
                     /locus_tag="MJF80_004035"
     CDS             5147..6070
                     /locus_tag="MJF80_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282575.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            6054..6824
                     /locus_tag="MJF80_004036"
     CDS             6054..6824
                     /locus_tag="MJF80_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282576.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            6834..7616
                     /locus_tag="MJF80_004037"
     CDS             6834..7616
                     /locus_tag="MJF80_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282577.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            7993..8529
                     /locus_tag="MJF80_004038"
     CDS             7993..8529
                     /locus_tag="MJF80_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBZ0282578.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8561..8950
                     /locus_tag="MJF80_004039"
     CDS             8561..8950
                     /locus_tag="MJF80_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBZ0282579.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            8987..9373
                     /locus_tag="MJF80_004040"
     CDS             8987..9373
                     /locus_tag="MJF80_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBZ0282580.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9500..10942
                     /locus_tag="MJF80_004041"
     CDS             9500..10942
                     /locus_tag="MJF80_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBZ0282581.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            11239..12054
                     /locus_tag="MJF80_004042"
     CDS             11239..12054
                     /locus_tag="MJF80_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBZ0282582.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         4377 a         1508 c         2312 g         3956 t
ORIGIN      
        1 gaagcaggaa ccctgtgact ttagtcatgg gaggttcagt ttgggcaagt agtttcttgt
       61 aaatctagtt atgtttcaac caatgataat ataatttcat tagtattagc tggtaattaa
      121 gttaatttga ctagagataa gtaaattgat ttaaaaggaa ataagtaaat caacaattaa
      181 atacatctaa aagtaagttt attgcaatga attgttaaag aaaataatta atatggagta
      241 aagctattgc aatgaagtaa caaagaaaat aattaatata gagtaagttt attgtaatga
      301 attgttaaag aaaataatta atatagggta aatctatctc aatgaaataa caaagaaaag
      361 agttaatata gagtgaactt attgcaatga atcaacaaag aaaataatca atataaaaag
      421 gagaaatatt tatgaataca ggatttccaa aagacttttt atggggagca tcatcttctg
      481 catttcaagt ggaaggagct tgggataaag acaataaagg aaaaaccgta gccgactata
      541 attcttttaa aaagtctcat ttacaggcag atactaaagt cgcatcagac ttttaccata
      601 attatgaaga agatattgaa ttaatgaaag aattagggat gaaaacatat cgtttttcaa
      661 tttcatgggc aagaataata ccagatggtg aaggagagat aaaccagaaa ggtttagaat
      721 tttataataa gataatagac aaactgatag agtgtgatat tgagccattc gtaactttat
      781 accattttga ccttccattt aaattagtag agaaatataa tggttgggaa agtagagaaa
      841 ctgtctatgc ttttgaaaga tttgctaaaa tatgctttaa gcattttgga gatagggtaa
      901 agtattggca acctcataat gaacagaatt taattgttag agtggaggag agaattaata
      961 tatatgatga aacagattca tggaagatag ataaaatgcg tgctcagatg gattataatc
     1021 tttgtttagc acatgcatta gcagtcaatg catgtcatga gatgataaaa gaaagtaaaa
     1081 ttggagcagc agtatcatca tctgtaacat atcctcttac ttctaagcca gaagatgtat
     1141 atgcagctag aatgaatgat aattttaaag tgtactatat gcttgatatg catcattatg
     1201 gagaatatcc agggtattat atgaagtatc ttgaaaaaag aaatatagtt cctcacatgg
     1261 aagatgggga taaagagata cttaaaaaag ctaaaatgga ttttatagct gtaaattatt
     1321 ataggacaaa ttgtgcagaa gctttacctg aagattcaca acatccattt ggattaagag
     1381 aaggaactgt tgattttagt atgtatggac tatttaaaat gtcaatgaat cctaacttag
     1441 aggcttctga gtatggagca gcaatagacc catcaggtct tagagttgcc ttaaatgaat
     1501 attggcaaag atatcactta cctgttataa taacagaaaa tggtctagga gctaaagata
     1561 tattagaaga tggaaaaatt catgatgact atcgtataga ctatttaaga agtcatataa
     1621 atgcatgtaa actagctatt gaagatggtg tagagatgat tggatattgt ccatggtcat
     1681 ttacagacct tttaagttct tctcaaggtt ttaataagcg ttatggactt gtttatataa
     1741 atagaactga ccatgaagtt ttagatttaa aaagaatcaa gaaagatagt ttttactggt
     1801 ataaagaagt tatagagaac aatggaatag taaaataaac ataaatatat attgagggtt
     1861 ttagtatgta cattacaaag gtattaaata attcgttgct acttgcaaag gatgataatg
     1921 atgaagaaat aatattgatg gggaaaggca ttggccataa ttataaggcc gggtctgagt
     1981 taaataaaaa agatatagaa aagatttttg ttcttcatga tgaaaatata aaaaagagta
     2041 ttatacaatt ggcaacagaa atagatgcag aatattttgg tatagctcaa atgataattg
     2101 catatggaat agaaaaatat aacttaaaac ttatgaacca tatatatttg gccttaactg
     2161 accacattgc ctttgcagtg agacgtttta aagaaggtat aatgattgaa aatcactatt
     2221 tgttcgaaat caaggaattt aatccaaaag agtatgatat aggaaaatat gctattactg
     2281 tttttaagga ggttcttggt ttagacctat cagaagaaga gattggtaat atagcaggcc
     2341 attttataaa tgcacaacaa gataatcctt atagtgatag aaataagaga agtgctaaaa
     2401 ttgtaaatgc tattttgcaa atagttcatt atcacttttc tattgtatat aataaagagt
     2461 cattttatta taggagattt gtaatgcatc taaaggcctt ttcacaaaga tttctggcta
     2521 atgaaccatc aagagaaaaa atagatttta tatatgagca agttcaaaaa aattgtaagg
     2581 aagagtatga atgtgtgaaa aaggtaggag cttatatgta taaggaatac tccagagaat
     2641 taccaagaca agaggagtta tatttgatga ttcatattca taagatttta ggagaattat
     2701 gcgaaaatag ttagaatatc ttaatatgct agtaatatta taaaaaatta aaaattaact
     2761 acactttaaa tatgaataat tgcattactt aagggggttg tcttaaaatt gattttttta
     2821 attaaatttg aagctcaact ccttttttaa acctttatat cattacaact ttaatgttta
     2881 tacgcctgta ctttagatgt atatctactt tttacatttc tcaacatgca aacccaccat
     2941 tttttgcagt atgttaattg aaataaacca ttgagttaac cctaaattta ttctggactt
     3001 ataggaatag ttccaaagat actttaaaat attttctgga ataattgctt atttcacata
     3061 tgttttattg tgatgttttg atttaataaa ttcttcaact ctgtatatag taaatgtatt
     3121 atcatgtttg ttttattcaa gcacacttat aatatgcttg attttttcac aatgatgaaa
     3181 tatactaatg ggtgttatag gtataagaat agcattacac ttgaagaagt aagtgataaa
     3241 tgagatataa atcctcatat gataaactac tatttcattg aggagcatat tctaagtgat
     3301 gtcaagatac tacaatatga ctaaattcct agtgataaaa aagtctacga atgagtgtag
     3361 aaaggggaac taagatgaat aaagttttaa ttatagatga tgataaagat ctttgtattt
     3421 tgttaaaaaa gagtatctca attgaacaga ttgaagcaaa ctattgccat tctgggagag
     3481 atggactggt tgaactttca aaacaagatt atcagttagt agttctagac gtgatgatgc
     3541 caggtatgga tggttttgaa actatggaac aaatacgaaa atatagcagt attcccatct
     3601 taatgctgac atcaaaaagc gataattaca gcaaagtgca tggattgcga gctggtgcag
     3661 atgactatct aaccaaacca tttgaaatta cagaatttac tgctcgtgta ctgtctttaa
     3721 tccgacgtta cacccgtttt aatccaaaaa atgaggagcc acagcaactt tctttcaaag
     3781 gtttgttgat tgaccttgac cagcatacag ttactaccaa tggcgaacct atgttgcttc
     3841 cacccaagga atttggtgta cttctttact gtgcaaggca tcagggtaag attttgacaa
     3901 aacagcagat ttatgaagcc gtgtggggtg agccatatgt atatgatgac agcaatatca
     3961 tggctattat cagtcgccta agaaaaaaaa ttgaactaga cccatcatct cccatctatt
     4021 tgcaaacaat aaaaggaatt gggtatcaat tcaacacgga ggtatgaaaa tggacgaaca
     4081 aacgataatt cttttcttac tcttgctgtc gattggtcta ctttttcttt ccatatttgc
     4141 tttttccaag cttgggcata tttacaagcg cctaaaagat atagaggaga tactggctga
     4201 tgtacaaatt ggagacgaaa atcgtaaaat actaattaag ccatgtgatg ttatggctcc
     4261 attggtttat cagcttaatg agattgtcta cgattatgaa aataagttac tttctctgaa
     4321 aaagtcggat aaagctagca aacaacttat gacaagctta tctcatgatg ttcgtacacc
     4381 attaacaact ctgattggtt atatggatgc tgtacacagt ggtatagtta tcggacaaga
     4441 gcgggaggaa taccttgaaa tcgctaggcg tagagcctac gacctcaagg attacattga
     4501 tgttcttttt gactggtttc ggttgaactc tgatgaattt acgctgtcaa tagaatctgt
     4561 ggaaattgca gagttgtcac gcaatatttt gaaagactgg attcctatat tccatgaaaa
     4621 gaaactgaat tttgaaattg atattccaga aaatcgacta atggtaaacc tcgacccaga
     4681 tggatattcc cgtgtggtca ataatctggt tcaaaatgtt cttgctcaca gtaaggctcg
     4741 gcaaatcaag attaccatga gtgaggattc cagaatggta ttactccgtg ttgaggatga
     4801 tggtgttggg attgccagag aaaatttacc acatattttt gagcgactgt ataagtgtga
     4861 taaaggacgt tcagaaaaag gaagtggtct tggtctatct attgtcagtc aaatggtgga
     4921 gcgaatgggt ggtcaggttt ccgtagaaag tgagattgga aagtataccg tatttacagt
     4981 gagcttacca ttaatataaa attgtttttc ccccatgtga catgtataca cagggggatt
     5041 ttttgtaagt tataaagttg caaggttaat acaaggttgt tgcaaggtta atgtaagatt
     5101 gaggtggtat gataacttta ataaacaaag gaggctttta ttatttatga ataatttaat
     5161 tattgaaaca aaaaatctca ctaagcaata tggtgctcaa aagagcgttt ctaatttgaa
     5221 tattcacgtt cagaaaggtc gcatttacgg attgctagga agaaacggtg cgggtaagac
     5281 gacaactatg aaaatgttat tgggtctaac aaaaccaact actggagaaa ttaaaatatt
     5341 tggtcaggac attcacaata acgagaagaa gcttcttcct cgaattggat gtttgattga
     5401 atcaccaggg ttttatccta atctgacagg aacagaaaat ctcaaaatct ttgcaaggct
     5461 tagaggaata ccaaaaaaaa atgcagtaga atcagcactg gatgtagtcg gtttgccaaa
     5521 caatgacaaa aagttatttt ctcaatactc tcttggtatg aaacagaggt tggcgattgc
     5581 tcttgccatt atgcatgacc cacaactgtt gattttggac gagcctatca atggacttga
     5641 tcctattggt attgcagaag taagaacgtt tattcgtgat ctctgcacag aacgaggtaa
     5701 aactattttg atttccagtc acattctttc tgaaattgcc ttgttggcag atgacattgg
     5761 cattattgac catggtattt tgttggagga agaaagtctt gcccaattag aagaaaaaaa
     5821 cagtaaatac atacattttg ctgtatctga tgcagcacag gcagcaaggc ttctggaaac
     5881 ccaacttaac ctgaaaaatt tcaaagtagc agataattac aatattatgc tatatgatac
     5941 agatgtttct gttgcagaaa taaatcgtgc tttcattcta aacaacattg aggtatcaga
     6001 atctcattta tgcgaagata ctcttgaaga ttacttcaag aaagtaacag ggggtgaagg
     6061 aattgcttga tataatttca tgtgaatttt caaaactcaa gcggttaaaa tttatactga
     6121 tttctatttt aggtgcttgc ttatttccaa ttcccgcaac aattttgatt gcaaaggaca
     6181 acttgccctt tgagcaattg ttcaaattag ttgtcaattt tggatatttt ttgctattac
     6241 caattgtatt aagtattgtt gctagccaac tattttttat agaaagagat aatgatgtat
     6301 taaaaaatct tgcaacagtg ccagtgcaaa aaggaaaact ggctctggca aaattggctg
     6361 tgttgctgtt tatatcattg ttctactctg ttgcagggtt aggagctact atcattggag
     6421 gtttcattgt tggaatagta gagggggttg ctgtaaaatt aggaatgagc attgcttttg
     6481 gaattatgtt ttttgttact gtacttcctg ttgtggtact cattgtatat ttcaatcgca
     6541 gctatatttt ttctattata ctttcttttg tatatgctat tttcaatttt agtatatcct
     6601 taaatattat caattttgaa ccaaataacc caatattaag tgtattacct gctcctgtta
     6661 taatgcgttg gtggatggca ttttggggag atcccactgg cgaatacacg gcacttagac
     6721 agccatattt gctctcaact cctgcttgtg taggtatttt atgtttgata accattatag
     6781 cagtcctgct gatttgtatg atatacaaaa agcaggagaa ttaggaggta tctatgcttg
     6841 atataattac tacagaattt caaaaaataa aaagatataa tattctatgg attggtgttg
     6901 ttgctgttct gttttcagca ctattggcag tttttcaggt tgtaagttca cacggcagtg
     6961 accctctaac gtatgagagc tttgcaaacg gagttatctg gaataatttc agtcttagtt
     7021 ttccatttgg gattacactt attggaggat atcttattaa ccgtgaatat acagaccaaa
     7081 ccttaaaaaa catcctaact gttcctattt cattacgcaa attgctagtg ggaaaattaa
     7141 ttgcggttgg aggaattact gtattgttaa gtcttttcag cttcttatgc acatttattt
     7201 tggggttgtt gttttgcaat atcaatatat ctgttacact cgttgtaaaa tccttagttc
     7261 agattttgtc tgttaatctg tattgttatg ttgctgtttt gccgattatt gcttattttg
     7321 gtcgtaaaca aaatgcattt ctgacgggtg tagggattgc atttgtatac ggtttttgtg
     7381 gtgtgtttgt tgctggaaga aatctgacag acttttatcc aatcacagca ggtcttggtc
     7441 tagttggata caacaatggt gctggtactg tttatcagcc acttatcggt gctatgactt
     7501 tgattgtaat tctaatcttg acaactattc tacttgtatt tacacctaat tatgataaag
     7561 ttatgaccat atctaagaaa aaagggataa agcataaaaa gtattctcat aaataacata
     7621 aatattgttt ctattattta agttgctaga cagtgaaaag aaaaaatctg tcgtacagtg
     7681 tctatatgct catgtattca agaagctaca gttttgattt ttcataactg tagcttcttt
     7741 ttgtatttat taaatcacga ctatcataat acttgcaata ggtatagcga caagagagag
     7801 tactgtcata tgtgttttat atattacatt aagtatattc taaaatatat gttttaaata
     7861 atatatttga aaataggaat atttaaataa aaaaaataat attatatata gttgacaaaa
     7921 attggcaaat gagatactat ataaatatag aaatagtaat cattactatt attaaaaagg
     7981 gaggaattaa ttatgaactt aaaaggaact aaaacagaaa agaatttaat ggcaggattt
     8041 gcaggagagt cagaagcaag aaataaatat acatactacg cttcaaaagc aagaaaagaa
     8101 ggttataatc aaatagctgc tatatttgaa gaaactgcta acaatgaaaa ggaacatgct
     8161 aaattatggt ttaaactttt acatgatggt atgccttcaa cagaggaaaa tttaaaggat
     8221 gcagcagcag gtgaaaacta tgaatggact gatatgtatg caaaatttgc taaagaagct
     8281 agagaagaag gatttgacaa gatagcttat ttatttgaag ctgttggaaa aatagagaaa
     8341 gagcatgaag aaagatactt aaagctttta gaaaacttaa atgaaggaaa aatatttaaa
     8401 agagatgaag aagttgtttg gcaatgtcaa aactgtggtc atgtgtatgt aggtactgaa
     8461 gcaccagaaa aatgcccagt atgtgaccat ccaaaagcgt atttcaatat aaaagctgaa
     8521 aattattaaa aattatcatt attaaaaagg aatgttaact atgaaatttt ctaaacaacg
     8581 agaactgatt ttaaatgaaa tattaaataa tccggttcat cctactgcgg attacctata
     8641 tgaaaactta aaaaaagata atccaaattt aagcttagga actgtgtata gaaatttagc
     8701 tcagctaacg gaacatggct ttataagaaa ggttagtatt ccagggtatc cagatagatt
     8761 tgatggtcga atagataatc actatcatat catatgtgaa gtatgtggag aggtatacga
     8821 tttagaatcc gaagttctta ataatttaca agaattaata tctgaggaga cagacataaa
     8881 aataacatct tataacataa gttttaaagg gatttgtaat aattgtaaaa ggtgtagcca
     8941 agtaggttag ttttcaaatt ctaaaaataa ttaatgaggt gagtttatgt gtagtgaaca
     9001 aaaatttttt atatgtgaga cttgtggaaa ccttgtagga atgatacaaa gtggtggtgt
     9061 acctattttc tgttgtggaa aacctatgaa ggaacttgtt cctaatacaa cagatgcagc
     9121 agttgaaaaa catgtacctg taattgaagt ggatggaaat aatgttactg ttaaagtaag
     9181 tagcacaact catccaatga ctaaagaaca tcatatagca tgggtgtatc ttatgactga
     9241 acaaggtggt caacgcaaat gtctagcagt agatggagaa cctgtagtta aatttgcttt
     9301 gaatgatgat gataaagtga tttctgccta tgcttactgt aatttacatg gattatggaa
     9361 ggctgaactg taatatttaa ataaattgag tcaaaaagga ggctataaat gtattttcat
     9421 acaaatagcc tcctataaat ttatataaat tcaagtttta attttgttta atgtcaatat
     9481 aatataagga gaaaataata tgtcaattta taaatgtagt gtttgtggat atatttatga
     9541 tgaatctaag aatgataaaa catgggatga attaagtgaa gattgggaat gccctgtatg
     9601 tacaaaaggt cgtagttatt ttggaaagat aagtactgtt tactatgaag aagatgaaaa
     9661 gatagcagaa gacatagttg aagatgaatc taaattaaat actgaaaaag agggagattt
     9721 aaattactta agtacatatt taagaagaga tgatgaagtt gagaaacata tggatataat
     9781 tcacgaaatg gctgttacag gaaaatctat aatagaacct atgagaacta aactaccagt
     9841 aatttcttgg gatgatattt taattatggg tgctcaatta aatccattac ctttaaatga
     9901 gcatgatgaa gtaaatacaa caacaataat aggaaaaaaa gcaaagaaac ctatgattat
     9961 agaaaatcca gtttatattt cccacatgtc atttggagct ttgtctaaag aactaaaaat
    10021 agctttagca aagggtgcag cccaaaataa gacagctatg tgtagtggtg aaggaggtat
    10081 attaccagaa gagaaggaag cttcatacaa gtatattttt gagtatgtac caaataaata
    10141 tagtgtaact gaagaaaatc ttaaaaattc tgatgctata gagataaaaa ttggtcaagg
    10201 aacaaaacct ggtatgggag gtcatcttcc tggcgaaaaa gtaactgaag aaatagcaaa
    10261 agtaagaaat aagcctgtag gtcaagatgt aataagtcca tcttgttttg aggaaataca
    10321 atccaaagaa gatttaaaga aacttgtaga tgaacttaga gaagtttcag aagggcgtcc
    10381 aataggtgtt aaaatttcag caggtcatat tgaaaaagat atggaattta tagcttatgc
    10441 taaacctgat tttgtaacta ttgatggtcg tggtggagct actggagcaa gtccaaagtt
    10501 gcttaaagat gcaacttcaa ttccaacaat atttgctctt tatcgtgcta gaaaatacat
    10561 agatacacat ggattagata tagatttggt tataacaggt gggcttcgta tatctactga
    10621 ttttgctaag gcaattgcta tgggtgctga tgcagttgct attgcaagtt ctgctcttat
    10681 ggcagcggcg tgtcaacaat atcgtatatg tggttctggt aagtgtcctg taggagtagc
    10741 tactcaggat gaggaattga gaaaaagatt acatatagaa aacagtgcta atagagtggc
    10801 aaacttttta aatgtaagtt tagaagaact taagactttt gctagaattt ctggtcataa
    10861 agatatacat gatttaagtg ttgatgattt atatactgtt aactctgaaa tatcaaatta
    10921 tacaaacata cagcatgtat agattgaagt ttgaaactat tagattttag atagctataa
    10981 aagtaaatga taaatatatt ttataaaaga gagtgtctta aaatgaactt tttagttcat
    11041 aagtcactct tttttttatg aagccaaata tatttatcat tttagcaatg aaaaagaatc
    11101 ttatctcttt attttgagat agactccttc tatttatgaa aattaaagat attaaattgt
    11161 aaaatattct gtgatgtcgt actattaaac taaagattct gtactttaaa gtaaataaaa
    11221 atttaaggag tgattattat ggaaagattg aagatacaag ttttagttga caataatact
    11281 tatattgata gatactttgt tggagaacca gcagtaagct attatattga gattgatgga
    11341 aataggattt tgtttgactc agggtattca gatgtattca tatcaaacgc tgaaaaatta
    11401 aatatagatt taggaaattt aactcatgtt gttttttctc atgggcataa tgaccataca
    11461 agaggtatcc agtttctaga aaataggtat gatttatcta cagttgaact tatttcacat
    11521 cctaactgtt ttatacctag aaaacatgga gagaaaagca taggagctcc attttctgct
    11581 gaggaaatta aaaatatttt tatgtataat cctaaggata agccatttaa tctaagtaag
    11641 aattgtgtgt ttttaggtga aataccttct ataaatgatt ttgaaaaaag agctaaaatt
    11701 ggtaaatgta aaataggaga tttgtgggaa gatgattatg ttttagatga ttctgctata
    11761 gtttgtaaaa ctgataaagg tatttttatt gttacaggat gttctcatag tggaatttgt
    11821 aatatagtag agtatgctaa aaaggtatgt ggagatgata gagttattgg tatactaggt
    11881 ggttttcatc tttttgaatt gaataatagg ttagatagta caatacaata tttggataaa
    11941 gaagaaatga gaatgttgta cccttgtcac tgtgtttctt ttaaggtaaa agcgaagatg
    12001 aatgaaatat tgaatataaa tgaagttgga gtagggctga ccataagtat atagtatttg
    12061 aaagtatata agatttaagt tgaaggttaa aaagatattt tctatagaaa atatcttttt
    12121 tgttacgttt attttaagag aaatcaatat act
//
LOCUS       DAGSWI010000348         8609 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.guided.157, whole genome shotgun sequence.
ACCESSION   DAGSWI010000348 DAGSWI010000000
VERSION     DAGSWI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.guided.157"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            <1..1061
                     /locus_tag="MJF80_004043"
     CDS             <1..1061
                     /locus_tag="MJF80_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBZ0282583.1"
                     /translation="ELTYYRPALSDGINEELGSDFYMEDKDWYDKNNIVVILGTNVDK
                     LDEVNKTIIVNDGAIKFDKLVIATGSRNFIPPIKGHDLENVFTLRNIKDLYSVKEALE
                     KSKKVVVIGGGLLGLEAAWEFRLKGLEVVVVEAMDSILSKQLDKEGSKILEQCVRDTG
                     IDVRLGVAVDGIEGDGKAQKVVFKDGDSVDCDMVVFSIGVRANTQMVQDTSVKIDRGI
                     VVDKTLQTNVKDIYACGDVAQVGNISLAIWPSSVEMGKIAGANASGDNLTFESEVYPV
                     SLDAMNVKVFSIGNIQNFDKEISSKDEGQRIYKKLFMKDGSLVGAILINDLSCTVKLI
                     RLISEKGDFEDIMKADIL"
     gene            1495..2196
                     /locus_tag="MJF80_004044"
     CDS             1495..2196
                     /locus_tag="MJF80_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HBZ0282584.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            2246..3328
                     /locus_tag="MJF80_004045"
     CDS             2246..3328
                     /locus_tag="MJF80_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0282585.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            3589..4689
                     /gene="vanG"
                     /locus_tag="MJF80_004046"
     CDS             3589..4689
                     /gene="vanG"
                     /locus_tag="MJF80_004046"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HBZ0282586.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            4686..5492
                     /locus_tag="MJF80_004047"
     CDS             4686..5492
                     /locus_tag="MJF80_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBZ0282587.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            5510..7648
                     /gene="vanT"
                     /locus_tag="MJF80_004048"
     CDS             5510..7648
                     /gene="vanT"
                     /locus_tag="MJF80_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HBZ0282588.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            7962..8582
                     /locus_tag="MJF80_004049"
     CDS             7962..8582
                     /locus_tag="MJF80_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBZ0282589.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
BASE COUNT         3183 a          926 c         1536 g         2964 t
ORIGIN      
        1 atgagttgac ttactataga cctgcacttt ctgatggtat aaatgaagaa cttggttcag
       61 atttctacat ggaagataaa gattggtatg ataaaaataa tatagtagta atattaggaa
      121 cgaatgtaga taaattagat gaagtgaata aaactattat agtgaatgat ggagctatta
      181 aatttgataa gcttgtaata gctacaggaa gtagaaactt tataccacca attaagggcc
      241 atgatttaga aaatgtgttt actcttagaa atataaaaga tttatatagt gtaaaagaag
      301 cactagaaaa atctaagaaa gttgtagtga ttggtggcgg tttattggga cttgaagctg
      361 catgggaatt tagactaaaa ggattagaag tagtagtagt tgaagccatg gatagcatac
      421 tatcaaaaca acttgataaa gaaggtagta aaatattaga acaatgtgta agagatacag
      481 gaatagatgt aagattgggt gtagcagttg atggtattga aggtgatgga aaagctcaaa
      541 aagttgtatt taaagatgga gatagtgtcg actgtgatat ggtagtattt tcaattggtg
      601 taagagcaaa cactcaaatg gttcaggata catctgtaaa aattgataga ggtattgttg
      661 tagataagac attacagaca aatgttaaag atatatatgc ttgtggagat gttgcacaag
      721 tgggaaatat tagtttagca atatggccat cttcagtaga aatgggaaaa atagcagggg
      781 caaatgcaag tggagataat ttgacatttg aaagtgaagt atatccagtt tcattagatg
      841 caatgaatgt aaaagtattt agtattggta atatacaaaa ttttgataaa gaaatttcat
      901 caaaagatga aggtcaaaga atatataaaa aattatttat gaaagatggt tcattagttg
      961 gagctatact tataaatgat ttaagctgta ctgttaaatt gataagatta atatctgaaa
     1021 aaggtgattt tgaggatatt atgaaggctg atatactata atttaaatac attaaaataa
     1081 aaaggagttg tctcaaaatt gatttttttc taatcaaatt ttaaattcaa ctcctttttt
     1141 gaatttttat ataaaataac atggaaattt tagtaatgtc tgtggttaca tacataggtt
     1201 taattgtttt ttatatggat tgtctctggt ataataaatt gaattgtgtt tgactgatag
     1261 atatttttat tgaaagttaa tacctgttat gcgataataa ggacgtgtta ggggagtttt
     1321 tataataaca attaatctca gtttttgtgt tccaatagta gcatcaacac attctgatgt
     1381 agtagttctt ttcttataaa aattagttac aaatatgctt gtaattattt gatatgtaaa
     1441 tttaccaata ttataaatga tgacctcgat aataaggaga gtgaaaaata atctatgaac
     1501 gaaaatattt tgattgtgga tgatgaaaaa gaaatagcag atttaattga agtatattta
     1561 aaaaatgaag gttttacagt atacaagttt tatacaggta aggaagctct agaatgtatt
     1621 gaatctaaaa atttggatat ggctatttta gatattatgc ttcctgatat tgatggattt
     1681 catatttgcc agaaaattcg agaaaaatac tattatccta tcattatgtt aacagctaaa
     1741 attgaagata tagacaagat aatgggttta accataggtg ctgatgatta tataacaaag
     1801 ccatttaacc ctttggaagt tgttgcaaga gtaaagacac aattaagaag atatatgcga
     1861 tataataatt cttatgaaca acagtcgata attgttaatg agtatgatat taaaggattg
     1921 attatcaata aagaaacaca taaatgtagt ttatttggga aagaagtagc attaacaccg
     1981 attgaatttt ctattctttg gtatttatgt gaacatcaag gaaaagtggt tccttctgaa
     2041 gaattgtttg aagctgtatg gggagaaaaa tatcttgata ataataacac agttatggct
     2101 catatagggc gattacgtga aaaaattcat gaaccttcta aaaacccgaa atttataaaa
     2161 actgtatggg gagtaggtta tacaattgaa aagtaaaaat attaaagtca atcctgactt
     2221 tactcaagtg agaaataaaa tttttatgag aacagcttta atggcattta tagccattat
     2281 tttcatatgg attttatatt cttttatatt gtatggtaag ttttctatct ggatagtatc
     2341 attctttgaa aatgcttttc gtatggaata taaagctgca ttgaatttat atcagcagat
     2401 tttccgtaat agcatggagc tcttcatgat actagctatg gtgattgtgt tttttataat
     2461 attccgggtt tatttaaatt ggtttacaaa atattttagt gaaataaatc aaggtattga
     2521 ctctcttatc aaagaagatg ttggagaagt ggctttatct ccagagttat tggcaataga
     2581 aaagaaaata aactcaatta aacatatact tgaacaacgt aaatttgaga cacaaatggc
     2641 agaacaacgt aagaatgaac ttattgttta tcttgcacat gatttaaaaa caccacttac
     2701 atcagttata gggtatctga cactacttcg agatgaaagt cagatttctg aagaactgcg
     2761 aaaaaaatat ttatctattt cgttggataa ggcagagcgt cttgaagatt taattaatga
     2821 attctttgaa attacaaggt ttaatttatc aaatatatca cttgaataca gtacagtaag
     2881 cttaactcgt atgcttgaac aactaacata tgagtttaaa cctactttga tgagtaagaa
     2941 tttaaaatat acactgaatg ttacaccaaa tatgatgata aggtgtgatg ttaataaaat
     3001 gcaacgtgta tttgataact tattacgaaa tgctgtttat tatagttttg aagatactac
     3061 tattgaaata acagctgtac aggaggatga ttatataaag cttaaattta taaatcatag
     3121 caatacgatt ccagaggaaa aacttgagcg tatttttgaa caattttaca ggttggatac
     3181 atctcgtaat tcaaacagtg gtagagctgg gttgggactt gctatagcta aagaaatagt
     3241 ggagcttcat aacgggacaa taacagctca tagcgagaac aatataatta tctttaaggt
     3301 tattattccg attgtaggaa atttgtaaga atttaagtat aaaaaaatag gaaattcttt
     3361 aggataaata aataaagagc actcttgtta ttgatataat tactgtatca aaacaagagt
     3421 gcttttattt tgatacagta attatatcaa tagaaaaatg tgaaaatata ttatgtggaa
     3481 cagatgcaat tgtaagtgaa ggttgattgg atttgaaata gaaaaagtat tttaacatgt
     3541 ttctgtagat tgtataaaaa taaataaatc aacaaaggag aatcaaagat gaataataaa
     3601 aaaattgcag ttatatttgg tggaaactca acagaatatg atgtatcatt acaatcagct
     3661 tttgcagtac ttgagaattt gaatacggaa aaatatgata ttataccaat tggaattaca
     3721 agggagggta attggtatca ttatattgga gattataata atatccagaa taacacttgg
     3781 tttgaaaggg atactgatat aattccagtt attgtttcgc agaaccgtgt caaaaatggt
     3841 attattgaat ttttaattcc tgatgctgtt tctcagaatc atattccaaa tagtgctatt
     3901 gaatcatgtg aaaatgatat taaatacact aaacttgatt ttgtgtttcc tgtactgcat
     3961 ggaaaaaatg gagaagatgg aacagtgcag gggttatttg aattggcagg tatacaggtt
     4021 ataggatgtg gtactctttc ttcagcatta tgtatggaca aagacaaatc acataaactt
     4081 gttcattcag aaggtattaa agtaccaaaa tcaatattac taaaatactc agataaattt
     4141 aatgtggctg aactgtcaaa agacttaact tatccattat ttataaaacc tgtgcgtgca
     4201 ggttcatcat ttggtattag taaaatttat aaaaaagaag agttacaagt agcagtagac
     4261 ttggcttttg aacatgataa tgaagtaata attgaggaaa atattgatgg ttttgaggta
     4321 gggtgtgcag ttttaggaaa cgatgactta cttgttggaa gagtagatga aatagaattg
     4381 tcaaaaggat tttttgatta tacagaaaaa tatacattga aaagttcaaa aattcatatg
     4441 ccagctagaa ttgatttgga ttgtgagaaa agaatacaag agacagcaaa aattatttat
     4501 agaactcttg gatgttctgg atttgctaga gtagatatgt ttttaactcc atctggtgaa
     4561 attattttta atgaagtcaa tacaattcct ggctttactt ctcatagtcg ttatccaaat
     4621 atgatgaaag gtattggatt gtcttttgaa gaaatattgg acaagataat tgaggtgtac
     4681 ataaaatgat gaataatatt ttattagata aaaataaaga agataaaatg acaaatacta
     4741 tcttattgac taaagataaa gtttatgatg ggaatctaat tctagtaaat gcttttttac
     4801 cagttaaaac atctgaagat atagatttaa ttcctgtaga tacaaggttt ccaagtattc
     4861 ttatgaaacg tgaagctaca aacattttac aaaatatttt aaaaagtata tgtggaataa
     4921 atgaaatagt acctgtaagt ggatatcgta ctgcagagga acaacaagat atatattcaa
     4981 gttctctgcg tgacaatgga aaagacttta ctaaaaagtt tgtagcactt ccaaatcaca
     5041 gtgaacatca gacagggctt gctattgatt tagggttaaa gaaagaagtt atagatttta
     5101 tctgccctga atttccttat aatggtattt gtaatgagtt tcgtaaggca gcactacatt
     5161 atggttttat agagcgttat cagtgtaaaa aagaagaaat aacaggaata tctcaagaac
     5221 catggcattt tcgttatgtg ggatatccgc attctgagat tatcaataaa atgaatctcg
     5281 ctcttgaaga atatatagaa tatattaaga gattttcgta tacaaattgt ttaaaaatag
     5341 aagataaaga aaaaattata gaaatctttt atgtgcctat tcatactaaa gaagaagtga
     5401 gaatctctat acctgaacaa tgtgtatatc aaatatcggg taacaataag gatggtttta
     5461 ttgtgacatt ttggagaaat agaaaggact aagaaatgga ggaacaagaa tgattaggaa
     5521 taaatcttat gcagggcttg attattttag gattatagct gcattactta taattgcaat
     5581 tcatacatct ccattattat catatagtaa aatggctgac tttgtgttaa ctagtataat
     5641 tgctcgtatt gctgtgccat tcttttttat gctatcaggc ttttttctaa tttcaacata
     5701 taattataat tgtaataaac tgaaatcttt tctaaaaaat gtattattta tttacgggat
     5761 agctattgta atttatattc ccttaaatat ttacactgga tatttttcca tgaataattt
     5821 agtgcccaat attattaagg acattgtatt tgatgggact ttatatcatt tatggtatct
     5881 acctgcatca attattggtg caagtattgc atactttctt gtaaaaagac taggtttaaa
     5941 gtactcacta gtaatcacta ttgttattta tgtaattggt atgtttggtg atagttatta
     6001 tggtttttta gaaaaaatac cactattaaa gaatttatat gggtatattt ttgaagtatc
     6061 agattataca cgaaatggta tattttttgc acctgtattt ttcatacttg gtggaataat
     6121 atctaataaa tctaattata tgtcaactaa aaatagcttg attggatttc ttatatcatt
     6181 cttacttatg ttatgtgagg gaatgttttt acataaacta ggggtacagc gtcatgatag
     6241 catgtatatt atgttattgc catgtatgta tttcttattt acagccttaa catttttgaa
     6301 aggttctcga gctaggttat tgcgaacatc atcattaatt atttatatta tacatcctat
     6361 gatgattgtt gttattcgta tgttttctaa aatattcgga atacaaactg tcttggttga
     6421 caatagcctt attcattata tagtggtaag tatagcatct tttatagctt ctataattat
     6481 tatattgatt cttaaacctc taaaaaaaga cttgaaagga gaacataagg taaataccaa
     6541 tagagcttgg attgaaattg atttaaataa tttggaacat aatgtaaaga tgatacaaaa
     6601 agctatgcca gaagattgtg aattgatggc agttgtaaaa gctaatgcct atggtcatag
     6661 tgcttttgaa gtagcgactt gtgcaaacca aataggtgta aaagcatttg cagtagcaac
     6721 aatagatgag gggattgatt tacgtcgata tggaatacta ggagaaatat tgattcttgg
     6781 atatactaac ccaataaggg caaaggaact tcataaatac aatcttacac agagtgttat
     6841 tgaccttaat catgctatct ctttaaacaa acagaaatac aacataaaat cacacattaa
     6901 aattgataca ggtatgcaca gactgggttt tgatgttaat gatgtgaggt caattttaaa
     6961 ggcatttaaa ttaaaatact tagatatatg tggcatattt acacacttgt gtgtttcaga
     7021 tagtctgaaa gatgaagatg tagactttac aaataaacaa attgaatgtt tttataagtt
     7081 aattgatttt ctgttaaaaa aaggtataaa aattcctaaa gtacatattc aaagtagtta
     7141 tggttttttt aattatccca atcttaagtg taattatgta agaattggaa ttgcattata
     7201 cgggatatta agttctccaa atgatacgac taaattgcaa cttgatttac gacctgtttt
     7261 atccttaaaa tcacaagtaa ttcttattag aaaaatacaa aaagatgata gttttggata
     7321 tggaagagtc tttattgcaa gcagagatag cgtaattgct atattatcta ttggctatgc
     7381 cgatggtata cctagaaatt tatcctgtgg aaaaagttac gtaataataa atggttgtag
     7441 agcagcaata gtgggtcgaa tttgcatgga ccaacttgct gttgatatta cagatatacc
     7501 taatgtagaa gttggtaata cggcaataat aataggaaga gataatttat ctgaattatc
     7561 tgcttctgaa gttgcaaata actcatgtag tatatcgaat gaattattga gcagagtagg
     7621 aagaaggtta aatgttataa agaagtaact caattaaata gtaatatttg ttgtactgtg
     7681 taatttattt tactctgact agacattttt agtaatatta tgtctaatat atatttttta
     7741 taacttacat gacaatttaa gttataaaaa ggtgatgttt cagattttta ttttgaaaca
     7801 tcaccttttt tgttataata tataataaaa taattatacc ttaactgtaa acttatatag
     7861 attatttact ttttaatatt agattcattt tttgagattg gtatataata aacatataga
     7921 gtatataata atagttaaag ttaaaaaagg ggaaaagcaa tatgcagtat atacaagaac
     7981 tacttgattt gactcaaaat tattgggtgt tagccacaat aatcggctta ttaagtgcat
     8041 ttatagaaag ctttatacca gccttaccac ttgttgctat agttacagca aatgctgcaa
     8101 tacaaggatt atttattgga tgtttgttat catggattgg ctcaggactt gggacaactt
     8161 cattattctt attaataagc agatttaatg atagtaagct ttttaataag ttgagaaata
     8221 gtaaaactga aaaagcaata agttggatgg ataaacaggg atttaagtta ttatttatag
     8281 cgtatgcctg tccatttatg cccggttgtt tagttactat cgcatcagct tttggtaaaa
     8341 aagatttaaa agattttgtg ccagcaatgt tggcaggaaa atttgtaatg tttatagtta
     8401 taagttacgt ggcaagtgat atagaaggtt ttataacaag tcctttaaag atagcatcat
     8461 ttatattatt ggtgtttttg tcatggaaga ttggaaatag agtaaataaa aatcttgaaa
     8521 atcataatta tgatttccat cataaaaaac atcacaatga taaagatgat aaaatgattt
     8581 agcttgtacg aaataaaacg tatcttaaa
//
LOCUS       DAGSWI010000349         8270 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.guided.163, whole genome shotgun sequence.
ACCESSION   DAGSWI010000349 DAGSWI010000000
VERSION     DAGSWI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.guided.163"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1334)
                     /locus_tag="MJF80_004050"
     CDS             complement(<1..1334)
                     /locus_tag="MJF80_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBZ0282590.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYT"
     gene            complement(1348..2277)
                     /locus_tag="MJF80_004051"
     CDS             complement(1348..2277)
                     /locus_tag="MJF80_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBZ0282591.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            2506..2694
                     /locus_tag="MJF80_004052"
     CDS             2506..2694
                     /locus_tag="MJF80_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0282592.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            2687..3388
                     /locus_tag="MJF80_004053"
     CDS             2687..3388
                     /locus_tag="MJF80_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282593.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            3390..4313
                     /locus_tag="MJF80_004054"
     CDS             3390..4313
                     /locus_tag="MJF80_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0282594.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            4470..5393
                     /locus_tag="MJF80_004055"
     CDS             4470..5393
                     /locus_tag="MJF80_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282595.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            5386..6123
                     /locus_tag="MJF80_004056"
     CDS             5386..6123
                     /locus_tag="MJF80_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282596.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            6134..6907
                     /locus_tag="MJF80_004057"
     CDS             6134..6907
                     /locus_tag="MJF80_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282597.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(7448..8056)
                     /locus_tag="MJF80_004058"
     CDS             complement(7448..8056)
                     /locus_tag="MJF80_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBZ0282598.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         2751 a         1360 c         1226 g         2933 t
ORIGIN      
        1 gtatagtcta aaaaactaga aaatcccatc actctatttg gtatgccaac ttctgttaga
       61 gcatttgcaa ttatgtaagc ttgaattgcc acatttccct gatttctact ttgagagcca
      121 cttgcatcca ataatatatc aataacgtat ttcccttttt cattttctat atctttataa
      181 aatattttat tattattact tctaccaact ctccaagctc tatttgctaa tatagtacca
      241 caatcagaat atactctact tttagcattt tcttctatta aaattcttga tagactttct
      301 tttaatctct ttatttgctg cctatacatt ctagcatgtt ctctaaattt tcctaaatta
      361 ttttcttttt gtcttgttac atattttagt tgaaatacat tattacactc acttctaaga
      421 actccatctg taaagtgtat tctacaacct tcatgaacat ttctacaatt ttgtgtctca
      481 atttttttaa tttcaccttt actcaaataa gtttttccat aataatgttc tatttttgaa
      541 tatatcttat ttgcagtgtc ttcatcaaca tatataactc ggtctgctga attgctacat
      601 ttagaatcat caaaatcccc tacaccttct acaagcatgg atgaagatat tgaatctata
      661 tcactttcaa taatattttc ttcttcatca ctataaagtt cttcatacat gaaatcagca
      721 aatgtatcaa aatttacttc tacactttct acattatttt catcaataag actattttct
      781 tctccattta atatgtattc aaaataagtt aaatatataa catctaatgc ttttaaaatc
      841 tccataatat cttgattggt atctatattt cttatatcat taactattct tctagtcata
      901 ctatctaatg ctgggtgctt gtccatacta taaagtacaa atgtgtattt aattttatcc
      961 ataatattat cattgtgtat tttggtaaaa cgtgaaacaa tatctttata agcatttctt
     1021 cttatgtctt ctatccctgg cctatcttta atgacctttt cttcaactat tgtatttaat
     1081 ataacttgaa ttagacccag tataatatct ttgttgtatc cttttcttat tctatttgta
     1141 agatattttt taactatatc ccaatctaca tactttcttc tagcacctgc aattattgca
     1201 aaatatagac ctatttcttt tgaagagtaa tctttttctg atatatctat actttcattg
     1261 taatcaccag ctattgtcca tgataagtta ttagctctat tttcaaattc ataatcatca
     1321 tatatccatt tcataatctc acctatatta aatatgactt attggaaaca catctatact
     1381 ctcccattta ttaggtattc tagttttaat tacatctcca accatttctt tctcatatac
     1441 atcaaaagtc tttcctgtaa gtcccatatt aattgcaaga gttggtttta atcctcttct
     1501 tatagtctta agagatgcca taagaccacg taaatcaata gctttactag atatctctcc
     1561 attttgagac ttcaattgta aatctaaaaa tattcctgca aagtgtatta gtttttcttc
     1621 atcagcatct gaaaattcat tttttaaaat catcattaac ttatcttctt ctataggagg
     1681 aatatctatt accataaatc tagataccag tgcttcatta agttcttttg ttcctgcata
     1741 ttcataattc atagtcccaa taaatcttgt tgctggatgt agattgactc tctcataccc
     1801 aggtacatca attattcttc tatagtctaa agctgaatgt accacaacta tagcatcatt
     1861 ttttgccata ttaatttcat caaacacccc aaaacctcca ttaatagcac actcataaac
     1921 tgagcctctt ctaagtttaa cttcattgtc tataaatgta tctgtaccta taagtgttga
     1981 actgtctgta tttatatgaa atgatgtatt ccactgaggt ctattaaata tttcccctag
     2041 attatcagca agtaagtttt ttcccgttgc ctttggtccc gataataata tattttcttc
     2101 ttctaaaata gctgctatac acatagacaa gatatcttta ccataaaaat aagttttaga
     2161 ctttgttact ctgtattcta aatctttttc taatccataa taatttctaa aatacagtac
     2221 atcttcaata agtttttcat ctaccccttg cacccttaga ttatctatta tatccatatt
     2281 tttacccctt ttatataaat agtatttgtt tcatttctta tcaccatttt agcataaaag
     2341 gatataagct aaaattgatt ttttaatctc ccccatttta agtaaaacca tgcaccattt
     2401 tatcactaca aaattaagag tactacttac tgatatttct tgattttatt cgtctatacg
     2461 tatataatat atactgataa attatgagga aaggatgatt gaaatatgga atatatgtct
     2521 tgcccagaag tagcaaaaaa atggggtatt tctgaaagaa gagtacaaaa actatgtgaa
     2581 gaaagtcgca taccaggagt ttcaaaactc gggtatatgt ggctgatacc aaagaacgca
     2641 gaaaagcctg ttgatggtag aacaaaaaga agaaaggaag tacaatatga atagcgtttt
     2701 gattatagat gatgacaaag aactttgtgc tcttatgaaa aaatgtgttg agcaagaaaa
     2761 tttatctgct attactgcac atggtggtgc agaaggtcta catttagtcg ataaaaatag
     2821 acacacactc tctcttgtaa ttttggacat aatgatgcca gatatagacg gttttcaagt
     2881 tctgaaaaaa attagagaaa caagtaatat tccagtaatt atgcttactg ctaaaaatga
     2941 cgaggatgat aaagtttccg gactacgttt aggtgctgac gattacctaa caaaaccgtt
     3001 cagcataaac gaacttatgg ctcgtgtcaa ttctcttatt cgtcggtata ctatgctaaa
     3061 ccctgctttt ataacagaca cagatttcat aataataaat ggcatgatta ttgacaagac
     3121 aaaccgaatt gtttctgtca ataatattcc tgttcaactt acaagcaaag aatttgattt
     3181 acttgctttt ctggcttcta ataagggacg ggtgtttacc aaaaaacaga tttatacaca
     3241 agtatgggaa gaagaatatg cttttgacga tagtaacatc atgtctttca tcagtaaatt
     3301 gagaaaaaag attgaaccag accccaacca tccattttat attttgacag ttcgtggtgt
     3361 cggttatcgt tttaataagg aggcttaaca tgaatacaag tgtctatctg cttttgatat
     3421 tggctcttag cctgattatt atagcgtacc ttgtttctgt cctgctacgt gtccgaatac
     3481 aacttacact tataaaagat gctttagagg atataaaaaa tggaaacctt aatcgacgta
     3541 ttttaacaga tgaaaacgac attacaaaac aaatttgcta tgatattaac gaaatagcaa
     3601 tcaatagcca atttcaactt attcagcaaa aacaatcaga acaagcctac aaacagctaa
     3661 tgacaagtct ctcccatgat gtaaaaactc cacttgcttc tttggtcggt tatttggaag
     3721 cagtagaaag taaaattgtt gtaggagagg aaaaagacga gtatatccat gttgcgtcta
     3781 ataaggctca ttacttaaag aattttgtag aaaatctatt tgaatgggta aaattggatt
     3841 ctaaggaaca agtttttcat tttgatattt ttgacttgaa tgaattatcc cgtaatatta
     3901 tatctgattg gatacctgtt ttagaaagca gtcattttga atatgaattt gatataccag
     3961 aaatagagta cttcttacgt atagacgcaa atgcatatac ccgtatcatt aataacctat
     4021 tacaaaatat tattacccat agtagtggca acaaaatgac attgagaatt tttgaaaaca
     4081 aagagcaagc tcaaattata ataacagata atgggaaagg tatatcttca gacaatctcc
     4141 cacatatttt tgaaaggttg taccaatgcg accattcacg agcttcaaag ggaaatggtt
     4201 taggcttggc gattgcaaag gaacttatta acgctcataa gggaaatatt accgccaaca
     4261 gcactcccgg tatgggaaca gaatttacaa tattgcttcc taaagccctg taaaagtacg
     4321 aggctttctt actctaaaac aagataaaag caagattatg gcaaggtttt ggcaaggttc
     4381 atgttttata attctaattg tagttgattt tgacgttgta tcaactatct tttgtagaaa
     4441 caaatttatt aaaagtgagg aaattatata tgaatgattt tgtgattgaa acaaagcaac
     4501 ttacaaaaat ttatgggaat caaacagtag ttgacaaagt caatctccat gtaaaaaaag
     4561 gccgaatata tggtctgtta ggacgcaacg gagctgggaa aaccacaatt atgaaaatga
     4621 ttttaggatt gacttcaatc acgtatggcg aggtagatgt atttggagag aatattaagg
     4681 gacgtgaaaa acaagtatat ccccgtatcg gtgcaattat cgaaacgcca ggattttatc
     4741 ctaatctgac aggaacggag aacttggaga tttttgcaaa actgcgtggt acagccgccc
     4801 ctaattctgt aaaaagagca ttggaaattg taggcttacc ttataaaaat aaaaagctgt
     4861 ttagtaaata ttcacttggc atgaaacagc gacttggtat tgccaacgct atcctgcatg
     4921 acccagaact gttagtttta gatgagccaa ccaatggtct tgaccctatt ggtattgcgg
     4981 agatgagaaa gtttattagg gacttgagcg ttgagcatgg aaagacaatt cttatttcca
     5041 gtcatattct ttcagaaatc tcattgttgg ctgatgatat tggaattatc gaccacggtg
     5101 ttcttttgga agaaagtagt atgagtgaac tagaaaagaa aaacagtaaa tatatcctgc
     5161 tacaagtttc agatattccg aagacatcac ttattttaga gcgtcaattt aacacgaagg
     5221 attattccgt tcaagatgac tacacgttac acctttataa cactaatctg gatatgggtg
     5281 aaatcaataa agcacttgta atgcagaatg taactgttat tagttctcaa ctttgtaatg
     5341 atactttaga ggactacttt aaaaaaatca caggaggaga gggcattgct taaacttatt
     5401 caaacagaat ttcttaaact gcgtcgtagg aaacttattt ggctgatgtt gttgtctgct
     5461 ctcattatgc cgtttttcgc actactatac ttcaattatt ttggtaaaac aggtgttgag
     5521 ccaataaagt tttataaatg gtctgcattt ggttatacac tttggattat tttgcctgtt
     5581 gttttgggta tattttgcac catgcttata cacgaagaaa accagtatga tatgctcaaa
     5641 cagctttgga ttgtacctat tagtaaaatg ggatactttt ttagtaaatt tttcgttgtt
     5701 ttgatttact ctatttgttt tatgctgatt acagccgttg cttctgtgtt atttagtgta
     5761 ctttctggat atgtcgtatt tagctgggca agcattttat atttgttgaa aaaatgttta
     5821 gaaattggtg taatcactgc ttttgtaatg ttgccgattt tggcaatcgc tacatctcaa
     5881 aaaggataca ttctcccaat ttgtatcaca ttggtatatg ccttttccgg ttttgttata
     5941 atgacaataa atatgtattt acacccatta tccagtatgg gagttattat catgagaaat
     6001 aaagacattc caggtcttat attttcacag gaaattaatg tcccattagc atttctttgt
     6061 atttgtattt ggagtattgt atcagtattg tttgcaaata ttgcactagg aagaagaaag
     6121 tgaggtgtag ataatgtgga aattactttg ggctgaatgt caaaaaattc gtcgttcaaa
     6181 aattgtttgg attacagttt ttgcatccat tatgatagca gtcatagttt ttgcaggggg
     6241 gcaggatgta tataatgacc cagatttaca ttatggctta aagagcgttt atgacggttc
     6301 acggtatata gacaatgcag gttggtatat ggacgcagta caaccttggt caactttttt
     6361 tgttcttcct gctgtcattg ccctattagg aagctatatt atttgtcgtg aagaagagga
     6421 ggacactatt aaatccctgc gtcttattcc cataaatgaa acaaatttaa ccatagcaaa
     6481 aatgattatt acttttatat tcagtatact tctttatttg ttactcttta ctataacatt
     6541 tttgactgaa tccgttttac atttttccga tttatcgaca aaattggttt taagttgcat
     6601 gaaagagtat tttcttgatg gcataggtgt attctttgca atttctccca ttattgcctt
     6661 ggtatcgcgc atgaaaaaag gatattggct tgcattagta ttcacagaag tttattctat
     6721 tgctggacta tttgcgggta tgtcaaatgt cttacaaacc ttttttccta ttgcggcaat
     6781 atttaatatt tctggatact atataactac tggagaaaaa attatgggga gtatcataag
     6841 tttattactt tgtgcctgcc tttctgcatt tatacttaaa gggctaaaac acaatgaaaa
     6901 aaattgaaat aggtatatca aaatgaaaaa gataaaaaaa ctgattattc taattgtatt
     6961 cttgtttctt tttggcatgt tacactgtta tactgaatgg aaattcaaca ataacccacc
     7021 taaaggggta atagaaagaa taaatgagtg gactgataag attggactgg attagtaact
     7081 tatgttaatt atagtgaatg taatattagc aagttattgt ttcgtatttt aatactataa
     7141 ctttaaatat ttggttttaa tcttccaaac gaatataaaa aaactgtcac agaggcaaca
     7201 tatttttgaa tatttataaa gtggtagttt tgattttcaa agctaccgcc ttttcttcta
     7261 tataagcaaa cttatcctca acaaagtgta aaacatggta cattttaaga aaaaagtacc
     7321 acagacacaa gcaataaaag aaacgccgag aacctacaaa aacgtagtgc ttatgcagat
     7381 ttacgacgcc ttctattttc tcaaccgtct taatgacctt gtacaaaaga aatttttcta
     7441 atatttttta taatcttatc ttgtgatgtt ttttatgtat aaatgactta ataacaaatc
     7501 caataaaaat tatggatatt ggtataatta gaacgaggaa tttataatca ttataatgtg
     7561 acattattct tacccaactc tcagaaaaaa tatatcctat actgattaat gaagtattcc
     7621 atatggttat tcccacgata gatgatatta caaaagttat aatattttgc cctgctatac
     7681 ctgctataaa agatatgtat gttctacata aagggattaa tctacataca caaacagaaa
     7741 tagaagagta tttattaaaa taatcttgag atgcaaatat tcctttcttt gcctttggac
     7801 atatctctat tattttattt atgatagatt tacctcccca atttcccact gtataacaaa
     7861 atatcgaacc aagtatacct gcaattatac ttaaaattaa aattgtcaga aatccaaaat
     7921 gatttcttgc agcaattgct ccacataaag gtaaaacaac ttcgctaggt aatggaaaac
     7981 aagcatactc aatcataatt aatacaaata cagagattaa tccatattgt aaaataaaac
     8041 tattgattac agacacttac ttatctatct cctttcttat ttaaaggttt taagggtaca
     8101 tgttctaagg attctcaccc tatatttaaa gaatcaatct ctttaaacta ttatatgtat
     8161 ttacacaaag catttataac atactttaaa aatagtacct taaaataaga aacttaataa
     8221 ataaaaaaag attacgtggc tacgtcctac tctcccaggc agtttcccac
//
LOCUS       DAGSWI010000350         6827 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.guided.178, whole genome shotgun sequence.
ACCESSION   DAGSWI010000350 DAGSWI010000000
VERSION     DAGSWI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.guided.178"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="MJF80_004059"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="MJF80_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBZ0282599.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="MJF80_004060"
     CDS             1575..2258
                     /locus_tag="MJF80_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBZ0282600.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="MJF80_004061"
     CDS             2255..4915
                     /locus_tag="MJF80_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBZ0282601.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQVSVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..6277)
                     /locus_tag="MJF80_004062"
     CDS             complement(5096..6277)
                     /locus_tag="MJF80_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBZ0282602.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6277..>6827)
                     /locus_tag="MJF80_004063"
     CDS             complement(6277..>6827)
                     /locus_tag="MJF80_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBZ0282603.1"
                     /translation="KNFDKTIDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDD
                     EKDIIRGLDMGADDYVVKPFLLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKA
                     YLDNVEVPLTAGEYKLLLSLMENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLR
                     EKLKNPPCIKTLRGIGYRMEDS"
BASE COUNT         2554 a          861 c         1107 g         2305 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaatga taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtattacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaaggc gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atataaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatatca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac ccttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga ggatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatctatag accagctatt ttattagcag atgagccaac tggtaatctt gatagaaaaa
     2101 attcagaaga aattgtagac ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataag
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 tgataaaatt actctcccaa taggatatag aatcttaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcagttagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgcaaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctgtagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggc tgactgaaaa agatgcatca aaagaacttg cgaaaaatgg
     3901 aggatttaaa agtatagttt catctaaaaa atactctcct atagaacgtg atggcaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 agtttctgta gacccaaagt tattttataa tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtacagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaaa aggctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtagagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc tgagcaaacc
     5281 ccttaccatt atcttctatg ataatctcaa catatagagg attttgacta taataagcaa
     5341 ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt aaaagagctt
     5401 ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg atagtaaatg
     5461 tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta agaagagaat
     5521 gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt gataatgtaa
     5581 gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg ttaatataat
     5641 ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac atagaagtaa
     5701 ttggagtctt aagttgatga gcaatatctg caagattatc tgaaagagtt tgtctttctt
     5761 ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc tcatcttcaa
     5821 ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa ttcacttctt
     5881 ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt ctatgtttga
     5941 ttatatatat aactgtagcc attgttgaaa ttataccaaa cgatatcact agaaaataaa
     6001 aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa atttcatttc
     6061 ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt tctgtagact
     6121 taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat tcattaaaag
     6181 aagttatagt tgttagaatt acactcaata aaaaagttac aattaagcag aatattataa
     6241 aaacaactgt actgttgata tttcttggag ttttcactag ctgtcctcca ttctatatcc
     6301 tatgcctctt aacgttttta tacaaggtgg attttttaat ttttctctaa gacgttttat
     6361 tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga gcatttctag
     6421 taactttgta cgagtcatag ctctattttt attttccata agacttagaa gaagtttata
     6481 ttctcctgct gtcaaaggta cttcaacatt atctaaatat gctttgactt caaccttatt
     6541 caagcttata ttttcacatg taatagtatt gttagtattt gaatgagttc ttctaagaac
     6601 tgcattaatt ctggactgaa gtactgagag caaaaacggt ttaaccacat agtcatctgc
     6661 acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca aaaatatcac
     6721 tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac catcaggtag
     6781 atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttt
//
LOCUS       DAGSWI010000351         5129 bp    DNA     linear   BCT 17-FEB-2022
DEFINITION  TPA_asm: Clostridioides difficile strain RT5 isolate 15.7921506
            SAMN25516648-rid13961043.guided.204, whole genome shotgun sequence.
ACCESSION   DAGSWI010000351 DAGSWI010000000
VERSION     DAGSWI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25516648
            Sequence Read Archive: SRR17631242
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001250374.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001250374.1
            Genome Coverage       :: 67x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/17/2022 07:25:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,072
            CDSs (total)                      :: 3,987
            Genes (coding)                    :: 3,922
            CDSs (with protein)               :: 3,922
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 65
            CDSs (without protein)            :: 65
            Pseudo Genes (ambiguous residues) :: 0 of 65
            Pseudo Genes (frameshifted)       :: 31 of 65
            Pseudo Genes (incomplete)         :: 28 of 65
            Pseudo Genes (internal stop)      :: 20 of 65
            Pseudo Genes (multiple problems)  :: 12 of 65
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25516648-rid13961043.guided.204"
                     /strain="RT5"
                     /isolate="15.7921506"
                     /isolation_source="Human infection"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /country="United Kingdom"
                     /collection_date="2015-06-25"
                     /collected_by="ModMedMicOxford"
     gene            116..898
                     /locus_tag="MJF80_004064"
     CDS             116..898
                     /locus_tag="MJF80_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBZ0282604.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            1023..1949
                     /locus_tag="MJF80_004065"
     CDS             1023..1949
                     /locus_tag="MJF80_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282605.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            1963..2412
                     /locus_tag="MJF80_004066"
                     /pseudo
     CDS             1963..2412
                     /locus_tag="MJF80_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2772..3179
                     /locus_tag="MJF80_004067"
     CDS             2772..3179
                     /locus_tag="MJF80_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBZ0282606.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMQEH"
     gene            3181..3411
                     /locus_tag="MJF80_004068"
     CDS             3181..3411
                     /locus_tag="MJF80_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBZ0282607.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(3463..3672)
                     /locus_tag="MJF80_004069"
     CDS             complement(3463..3672)
                     /locus_tag="MJF80_004069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBZ0282608.1"
                     /translation="MVQVTAKQPFQRLFTNHYIVALLIKVMAYSICWLSVSQTYLSTL
                     FSCQRTVGIDLPIKNHNSLWAFIDK"
     gene            3801..4004
                     /locus_tag="MJF80_004070"
     CDS             3801..4004
                     /locus_tag="MJF80_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBZ0282609.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            4082..4435
                     /locus_tag="MJF80_004071"
     CDS             4082..4435
                     /locus_tag="MJF80_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HBZ0282610.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            4507..>5129
                     /locus_tag="MJF80_004072"
     CDS             4507..>5129
                     /locus_tag="MJF80_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBZ0282611.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNID"
BASE COUNT         1886 a          729 c          896 g         1618 t
ORIGIN      
        1 cttttttatt tcgggtcaag tgacctattt ttttttattt cttcattata taatactcct
       61 atgcataatt atgttggatt gatagctaat aaaagacagt gagaggtgtt atattatgac
      121 taaaacacaa cagaaaatga tacaaaacgt attagaattt tcaaaaagag atagtaggat
      181 aataggactt gcaattagca gttcttatat cacaaaatcg ttagatgaat attctgattt
      241 ggattttctg attgtagcag atgataaatc ctatgaagaa gttatcgaag agagattact
      301 tattgtggaa cagtttggaa cattagtttc tgcttttact ggcgaacacg ttggagaacc
      361 tcgattgata atttccctct atgattcccc tgttcttcat gttgatttta agtttacaac
      421 cttagatggt ttactagata gggttgaaga ttcagctatt ctctatgaag aaaacaactg
      481 cataacagaa atttattcta aaaaagaagc atacttccca actccagact tacagtggat
      541 tgaagataga ttttggattt gggttcatta cgcttgcact aagattggac gtagagaact
      601 atttgaaaca attgattttt tatctttttt aagacaatca gtacttgccc cattaataca
      661 gcttctctta ggtaaattac ctcgtggagt tagaaaaatt gaattggacg caccccaata
      721 tattgaagca ttagaaaaga cggttgctaa ccatgaatat aaaagctgtg ttaatgcatt
      781 gcaggctact atcaacctgt atcttgagtt aagagaggaa ttatcacaaa gtcctattgt
      841 ccgaaaggtt gaagctgaaa gggttgcatt acagtatttt caagatttaa tcaaataaac
      901 ccaaagtaaa aaaatattga atagtaaata ttcagtgttt tttcattttt ggtcaaatga
      961 cctagattag ttgttgtaac aattctataa ttattttgaa agtaacagta tggaggataa
     1021 ctatgagtga tattttaagt agaattacaa ataaaattgg ggataagaat atagttgata
     1081 agttatcagc tctatcaaaa tcagatttga actcacttct attagaagtg tttgatagac
     1141 aagcaaatac tttaactgca actgatattc taaagtcata ccaactaaat agatttacta
     1201 tccctagcgg tatagatcca gaagaaattc atgctttgga atcaaaatta ttgagaaaag
     1261 cattcaatat ggatatcaag acgattatgc tttctccctc tgccccatta ggtagctgta
     1321 gcgtttttgg ctccgtagat caatataacg tagtaagtgc tctacgaggg acagaaacac
     1381 ttgcagaccc ctcaaatatg ttggcaataa ttattgctga taaattaaaa agaaaagaag
     1441 aaaataatac catacctatc cacatggcta ctactgctag ggtaataaga gcacaaaatt
     1501 ttgtaggtaa aggtttatat tcacattttg gcttatattg tatggtttca tctggtatag
     1561 atacagggtc ttacacttgt gaaaaaatgt tgttagaaaa gcacttaaac tattataaag
     1621 atatattaag tgaaatagaa aatgttcgtt tctctattat tctacgcaaa agaaacggat
     1681 acaaagataa tgacggattt ttcgacagaa tggttgaaac tatcaaactt atttttccta
     1741 atatagagtt atcaattcat aatgatgatg ttgataataa ttattataaa ggtatcaatt
     1801 tcaaattatc ggtcaaacaa ggaaatgaaa ccgttgaaat agttgacggt ggatttgttg
     1861 actggactta tcaaatgctt ggaaataaaa gagaacgctg tttgataagt ggtattggct
     1921 tagaaagatt tcttgtagct gtttcataaa aggagaagaa aaatggaact tatagagtgg
     1981 aattatgctt atattaccga tttagttgag tttgcgaatg ataaagaaat tgcaaagaat
     2041 cttcgtgaca acttccccca cccttatagg gagcaaaacg ctagagattt tgttagtttt
     2101 tgtttggcaa cttcagatgt taaacaactt agctatgcta ttttttatca agaaagggct
     2161 attggaagta tcagcctaac atttgaagaa gatacctcta ttaataaaaa tgctgaatta
     2221 ggttactggc ttgctaggaa ctattgaggc aaagggatag caacaaatgc agtaaaagaa
     2281 atatgcaaaa ttgcctttga aaaatacaac cttaacaaaa ttcatgctaa tgtatattcc
     2341 tacaatagag agtcaagtaa agtattggaa aaatgtggct ttaagattga agaatactgt
     2401 cacaattcat aataaagaat agaaacatga ttgattgcca tgtatatagt ttaattcgac
     2461 cgcaatagca tttattagaa ttaacctact ttttaataac agaataagtt tttcccctct
     2521 attaatttct tatgcatctt tgtattttta tgaaaagcta gccaatgcat atttccatac
     2581 atcaatatgg tggtggctta caaccaatat aaattaaata cgttttaaag aatacacatt
     2641 tttgccctat aggcagagtg tgtatttttt tctctttttt ttgaaattta tcgaagattt
     2701 aacgccctcc caccgtgata ggagtaagca aggttattta actccttgcg atggaaagag
     2761 ggtgaaatct tatggttgat gaaaagacaa ttgaacatca attcgacagt ttctgtaaaa
     2821 cagttttgcg taactatgct agagatattt atgacgaaaa taaacgcaga aatgattatt
     2881 tggtatctct cgaatctttg tcacttgcag aattaagtaa attgagtatt ttagatgatt
     2941 atgattctaa ctacatctgt atggtttctt acgactacaa catacgaatt gaagatgtat
     3001 taatggcgca agcaatcgga aaacttacaa aaagaaaaca agatataatt ctactttcat
     3061 tctttttcaa catgacaaat gctgacatag caacattgat ggatttagca gaaaatacca
     3121 tacattatca caaaacaaat gcactaaaag agttgaaaga actcatgcag gaacattgaa
     3181 atgaaaccta ctaaagaagt attacctttt acaattattt tacttgctgt acatggcgat
     3241 gtaaatgcaa taaacgtaat acttaaacat tttgaacact atatcattcg tctttcacag
     3301 aaaactttat ttgatgaata cggaaatccc tatattcatg tagaagaaga aacaaagcga
     3361 atgctaggaa caaaactaat cactgctatt ttgagattta atttgtctta atctcgccct
     3421 ctctccatgt atcaagctat ttggtacatg gagaatcact aattatttgt ctataaaagc
     3481 ccacaagcta ttgtgatttt ttataggcaa atcaatgccg actgttcttt gacaactgaa
     3541 taaagtagat agatacgttt gtgatacaga tagccaacaa atactatacg ccatgacctt
     3601 aatcaagaga gctactatat agtgatttgt gaataacctt tggaacggtt gttttgccgt
     3661 aacctgtacc acagtataat gatacacctg tatgacacgg ttctgtccat aagaatggga
     3721 ttggttgaaa taccaatgga gcttatcaaa gccatctatc tacttctgaa ctacgaacat
     3781 agaaaggagt gatacattat atgaacaata ctgatgtacc tatttgggaa aaatatacct
     3841 tgacgattga agaagcgtca aaatactttc gtattggaga gaaaaaatta cgtaaacgtg
     3901 ctgatgaaaa ccttgattct ggctgggtca ttatgaatag caatcgcatt caaataaaga
     3961 gaaaacaatt tgagaaaatc gttgatacac ttgacgttat ctaatgaaat tgagccttat
     4021 atgttataat attcatatga cgtatcaagc tcaattcggt tattgagagg agcttcttat
     4081 catgtcaact gaaaaaagac gagataataa agatagaatt ttaagaactg gagagagcca
     4141 gagaaaagat ggaagatacg catataagta tgtatattca tttggtaaac cacaatttgt
     4201 ttactcttgg aaacttgtac ctactgataa acccccaaag ggaaaacgtg atgatatatc
     4261 attaagggat aaggaaaaag caatacaaaa tgaccttgat gatagcattg acactattgg
     4321 taagaaaatg acagtttgtc agttatacgc taaaaagaat ggactaagaa agaatatccg
     4381 acttaacact aaaaaaggac gccattatct tatgaagatt cttaacaagg agtgattctg
     4441 tgaagagatg tattgtagta gtggaatagt caaaaacata atatatatta aaggagagat
     4501 ttaaatatga ctattccaag ttcaaataag gtatatccaa gaagtaatga ttatcaaact
     4561 gtatatctta aaaatgtaat aacaagggaa aatatagaag ttggagatta cacaatatat
     4621 aatgactttt ataatgaccc aagagatttt gagaaaaaca atgtattgta tcattaccct
     4681 ataaacaatg ataaattaat aattggtaag ttttgctcaa tagcatgtaa tgcaaaattt
     4741 ctaatgacta gtggaaatca ctcaatgaaa tcaaaatcca catatacttt tcccatcttt
     4801 tatgaagaat ggaaactcga tatcaaccat ataacggacg cttgggacaa taagggtaac
     4861 attataattg gaaatgatgt gtggataggt tatgattcta taattatgtc aggtgtaaat
     4921 attggtgatg gtgcaattat tggaacaaga gcagtagtta ctaatgacgt tccaccgtat
     4981 tctattgttg gaggagtacc tgcaaaaatt ataaaaaaga gatttaataa tgatacaatt
     5041 tcaaaattat tagaaattaa atggtgggat tggccatata aaaaaattca atcaaacatt
     5101 aagcatattc aatcaggaaa tattgataa
//
